LOCUS       DAOQOC010000001         8914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_2899_length_8914_cov_4.276668, whole genome shotgun sequence.
ACCESSION   DAOQOC010000001 DAOQOC010000000
VERSION     DAOQOC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_8914_cov_4.276668"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PL106_00005"
     CDS             complement(<1..742)
                     /locus_tag="PL106_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31473.1"
                     /translation="MIPVHITFQRVQTWLFNVPRLRAMVGANTLIGEALREDLPQLAR
                     QTGQGWTLSTAEGTYAAADPHDPLAAHDDPAQDAREGILARDGGHFEAVFASGAEAFA
                     DAAESLLQAKAPGLRFQISINKEARIKSSAQVSTDLPVLAPCEWTGNGLASAIITQGK
                     DGHSVSLDVQQRHEAAQRSVRGTAKDLASLLSNVTRLKDLERPEDLGRLVSNGYMALI
                     HADGNGVGSAAGTDPVGRASFHHRNRVLL"
     gene            complement(766..1629)
                     /gene="csb2"
                     /locus_tag="PL106_00010"
     CDS             complement(766..1629)
                     /gene="csb2"
                     /locus_tag="PL106_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HNA31474.1"
                     /translation="MILQTAEPIESPVAGPHPAAGTMGCMARYALRSGALPRMPMLMA
                     FTAKVHGALCALSDTGKGSSPTFSGRSAAGTPLRRHEHAYLFCDAAGHDDAITHLYVW
                     AGEDLDAQALIALTRLSKVWGHGGPDVYLRLEGIGQAEVLAPAALLGPAQEWAAYTPF
                     ISSRHAKTYRDGRAKHDPHNGLLIGSAPHELARLLALDQRFAGAAIRTVQDDHPFQYG
                     TSRHAPGAFRTARTDRGGTRGESHGWAFTIRFPQPVAGPIALGFGAHFGLGMFRAVEP
                     KPQPGGTMNKT"
     gene            complement(1626..2669)
                     /locus_tag="PL106_00015"
     CDS             complement(1626..2669)
                     /locus_tag="PL106_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNA31475.1"
                     /translation="MPISKNAWIRYQALDRCFSNRVKNHYIADLVAACTAALEEHSAG
                     KTSISRRQVLEDIKFMESDQGWAILLDRIKDGPQVYFRYRKRNFTIANRPLNKEESKQ
                     LGEVLKFLSRFRGFPRFNWLEEATARLQGDMGLKPGTAQAMAFQENQELEGAMHIAPM
                     LDAIMARKVLHVTYRSFRQEQEESFLFHPHLLKQYNNRWFLLGCRDGREEVTNLALDR
                     VIKLKEAPEADYRASNTDWNDHFDDVVGVSVPDSPVEHVRIRVNTNTWPYIRTKPLHH
                     SQRVKETNEAAVEIEVRLKLNHELIARLLEHGPGVEVLEPKSLREIMKENIRQMASHY
                     GLADAVAQPSTKP"
     gene            complement(2706..3683)
                     /locus_tag="PL106_00020"
     CDS             complement(2706..3683)
                     /locus_tag="PL106_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HNA31476.1"
                     /translation="MFNFFCLLTGDRAQTVDQFGSNSRKKISMMAMGLLAVVVTWASM
                     SCLIGSQVLQLGTTHTVLLALVCTVLVFIVDRSIIQISHRSWGIGALRVFIALIVALV
                     GGTALDLVLLDGEIRQELVYMHEEQRAEMKRAIDLKYATAVQEAEHPVAQAQAAYDQA
                     ERDFKLEMNGAPQGTGLRGMGKVATEKKALWSARLQDLHNAQAVLVKLQGKRDGEVRV
                     LMAKQEKASIQPALFERIHALHRYVRRDTAVFCGWLLFTLLIVFIELLPTMAKFLGKE
                     TAYERSQRLVEELHQRHLEHRAGMRQEEVDARSTLRAVQQEYAHMRRSA"
     gene            complement(3686..4267)
                     /locus_tag="PL106_00025"
     CDS             complement(3686..4267)
                     /locus_tag="PL106_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31477.1"
                     /translation="MNRFIQLISRKPRTAPATAIATPVSAPINREIFIDTEIPMPAAA
                     PASSAPAQQPVAYVSRVKQLANRDRFQEGYSAGFDFHDLETGRKLVERIKAEIRLALN
                     EECDKLEGMIANLDRELSALGDEPAHSATIRKTQTQREDMERYRLTLEGHKLLVVGND
                     GFAELPVASFHAGFKQGYEKYLQEILFLNQYTR"
     gene            complement(4292..4573)
                     /locus_tag="PL106_00030"
     CDS             complement(4292..4573)
                     /locus_tag="PL106_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31478.1"
                     /translation="MSTTQTHNATYAEMLQHPKWKAKRASVLARDAHQCRNCGTRGNL
                     QVHHRQYHRHKATGYKLAPWCYPDRYLITLCDACHYQGHQQFTVPSHLI"
     gene            complement(5222..5479)
                     /locus_tag="PL106_00035"
     CDS             complement(5222..5479)
                     /locus_tag="PL106_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA31479.1"
                     /translation="MAGPVVLVDTSVLIDYFRKKDKGRTLLHQLAEDGCTLKLSVITE
                     YEVYVGATRASSRIGAECLSTLRCCHSDQGKCAVPQRSKPN"
     gene            complement(5464..5697)
                     /locus_tag="PL106_00040"
     CDS             complement(5464..5697)
                     /locus_tag="PL106_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31480.1"
                     /translation="MEVKLNMPFEQLLRALAQLTPTQRRKAKAVLERKPAGKTSEAEE
                     SLAELLLHGPVFSKEQLLRMEEARNAFATWRDQ"
     gene            complement(5923..6390)
                     /locus_tag="PL106_00045"
     CDS             complement(5923..6390)
                     /locus_tag="PL106_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA31481.1"
                     /translation="MSSYSKMHFHIVFSTQHRVACLDQRWREKLWAYMAGTINGLGGH
                     ARNVGGWKDHVHILADLKTTQTVADAVREVKKASTQWIRTNGRVAQFKWQDGYAVFTV
                     GWRELQPLAHYINNQEEHHRHRSFQEELITFLEEAGVEYDPKYLPQCATPSGS"
     gene            complement(6561..8498)
                     /gene="acs"
                     /locus_tag="PL106_00050"
     CDS             complement(6561..8498)
                     /gene="acs"
                     /locus_tag="PL106_00050"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNA31482.1"
                     /translation="METTIHKDTPFRRLRSRAEYDEAYRLSVQDPEAFWAAQAETFTW
                     REKWDKVLEWDFHKPEVKWFIGGKLNITENCLDRHLEKRGNKLALIWEPNDPGERFIR
                     YTYRELHEKVCQYANVLKRNGAKKGDRICIYMPMIPELTIAVLACARIGAIHSVVFAG
                     FSAKSIADRVNDARCSMVLTSDGLHRGHKQIPVKRVVDEALEECPSVKKVIVTDRLGW
                     AVDMQEGRDVWLHDELKHVDKHCPAEEMDAEDPLFILYTSGSTGKPKGVVHTCGGYMV
                     YCDYTFRNVFQYEESDIYWCTADIGWVTGHSYIIYGPLCAGATTLMFEGVPTFPDAGR
                     FWQVIDKHGVNIFYTAPTAIRSLMAHGLDHVLAYSLDSLKVLGSVGEPINEEAWQWYK
                     LHVGKNRCPIVDTWWQTETAGILISSLAGVTDEKPGHAAYPLPGIQPVLLTAEGKEIT
                     ANGEEGMLCIKFPWPGILRTTWGDHERYKQTYFSSYPGHYFTGDGAKRDADGRWRVIG
                     RVDDVINVSGHRFGTAEIESAINLSERVVESAVVGYPHDIKGQGIYAYVITAEPLDQK
                     DKQAVDKITGEIIESVVATIGRIAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGEMSN
                     LGDTTTLLDPGVVEEIKGGRV"
BASE COUNT         1517 a         2642 c         2797 g         1958 t
ORIGIN      
        1 gcaaaagcac acggttgcgg tggtggaaac tcgcacgtcc cacggggtcc gtgccagcgg
       61 cgctgcccac tccgttcccg tcggcatgaa tgagggccat gtacccattg ctgaccaacc
      121 gcccaaggtc ttccgggcgt tccaggtcct tcaaccgggt aacattgctg agcaagctgg
      181 cgaggtcctt ggccgtaccc cgcacactgc gctgcgctgc ttcatggcgc tgttgcacgt
      241 cgagggagac cgagtgccca tccttccctt gggtgatgat cgctgatgca agcccgttgc
      301 cggtccattc gcaaggggcc agcacgggga ggtccgttga tacttgggcg ctgcttttga
      361 tccgcgcttc cttattgatg ctgatctgga agcgcaaccc cggggccttg gcctgcaaca
      421 ggctttcggc ggcgtcggca aaggcctccg caccggaggc gaacacggcc tcgaagtgtc
      481 ctccatccct ggccaggata ccttcacggg catcctgtgc tggatcgtcg tgtgcggcaa
      541 gtgggtcatg cgggtctgct gcagcgtatg tgccttcggc cgtggaaagc gtccagcctt
      601 gcccggtttg gcgtgccagt tgcggcaggt cctcgcgcag cgcctcgccg atgagcgtgt
      661 tggcgcccac catggcgcgt aaccgaggca cattgaagag ccaggtctgc acgcgttgga
      721 aagtgatgtg aaccggtatc atgcgataag aggttgatcg gtttttcaag ttttgttcat
      781 tgtcccacct ggttgtggct tgggttccac cgccctgaac atgcccaatc cgaagtgcgc
      841 gccgaagccc aaggcaatgg ggccggccac gggttggggg aagcggatgg tgaatgccca
      901 gccatggctt tcgcccctgg taccaccgcg gtcggtgcgg gcggtccgga aagcgcccgg
      961 ggcgtggcgg ctggtgccgt attggaaggg gtggtcgtcc tgcaccgtgc ggatggccgc
     1021 accggcaaag cgttgatcca gggccaacag tcgggccagt tcgtgcgggg ccgatccgat
     1081 cagcagtcca ttgtgcgggt cgtgcttggc ccggccgtcg cggtaggttt tggcatggcg
     1141 cgaggaaatg aacggcgtgt aagcggccca ttcctgtgct ggtccgagca aggcagccgg
     1201 agcaagcact tcggcctgcc cgatgccctc caggcgcagg tacacatccg gcccgccgtg
     1261 gccccacacc ttgctcagcc tggtgagtgc gatgagggct tgtgcatcga gatcttcacc
     1321 ggcccataca tacagatgcg tgatggcgtc atcgtggccc gcggcatcgc agaacaggta
     1381 agcatgctcg tggcggcgca agggggtccc tgcggcgctg cggccgctga acgtggggga
     1441 agagcccttg ccggtgtcgg aaagcgcgca caaggcgccg tgcaccttgg cggtgaaggc
     1501 catgagcatg ggcatacgcg gcaaggcacc ggaccggagc gcgtagcggg ccatgcagcc
     1561 catggtgccc gctgcggggt gggggccggc aacggggctt tcgatcggtt ctgcggtttg
     1621 aaggatcatg gcttggtgga aggttgggcc acggcgtccg caagcccgta atgcgaggcc
     1681 atttgcctaa tattctcctt cattatttcg cgcaggcttt tcggctccag cacctccacg
     1741 ccggggccat gttccagtag tctggcgatc agctcgtggt tcaacttcaa tcggacctcg
     1801 atctcaacgg cggcctcgtt ggtttccttg acccgctgcg aatggtgcag gggtttcgtg
     1861 cggatgtagg gccaggtgtt cgtattcacc cgtatgcgca cgtgctccac ggggctgtcg
     1921 ggcacggaaa cccccaccac atcatcgaag tggtcgttcc agtccgtgtt tgatgcgcgg
     1981 tagtcggcct ccggggcttc cttcagcttg atgacgcggt ccaaggccag gttggtgacc
     2041 tcttcccggc catcccggca acccagcagg aaccagcggt tgttgtactg cttgagcaaa
     2101 tgcgggtgga acaggaagct ctcctcctgc tcctgcctga aactgcggta cgtgacgtgc
     2161 agcaccttcc gggccatgat ggcatcgagc ataggggcga tgtgcatggc accctccagc
     2221 tcctggttct cctggaaggc catggcctgt gcggtaccag gcttcagacc catgtcgcct
     2281 tgcagcctgg cggtcgcttc ttccagccag ttgaatcgcg gaaatccgcg gaaacgggaa
     2341 aggaacttca acacttcccc gagctgcttg ctttcctctt tgttcagcgg tcggttggcg
     2401 atggtgaagt tccgcttccg atagcggaaa tacacttggg ggccgtcctt gatgcggtcc
     2461 aataggatgg cccagccctg gtcgctttcc atgaacttga tgtcctccag cacctgtcgc
     2521 cgcgaaatgg acgttttgcc ggcactgtgc tcttccaagg ccgcagtgca tgccgcaacc
     2581 aggtccgcga tgtagtggtt cttgaccctg ttgctgaagc aacggtccaa ggcttggtag
     2641 cggatccagg cgttcttgga gatgggcatg gcaacggact tgcggggaaa ataaacagcg
     2701 gcatcttatg cgctccgtcg catgtgggca tattcctgtt gcacggcgcg cagcgtgctg
     2761 cgtgcgtcca cttcttcctg ccgcatgccg gcccggtgtt ccaagtggcg ctggtggagc
     2821 tcctccacga ggcgctggct cctttcataa gccgtttcct tgccaaggaa cttggccatg
     2881 gtgggcaaca gttcaatgaa gacgatcagc agggtgaaca acagccaacc gcagaacacg
     2941 gcggtgtccc gccgcacata gcgatgcaag gcatggatgc gttcgaacag tgcgggctgg
     3001 atcgatgcct tttcctgctt ggccatgagc acgcgcacct ccccatcgcg cttgccctgc
     3061 aacttgacca acacggcttg cgcattgtgc aagtcctgta gtcgcgcgga ccatagtgcc
     3121 ttcttctccg ttgccacttt gcccatgcca cgaaggccgg tgccttgcgg ggcgccgttc
     3181 atttccagct tgaagtcgcg ttccgcttgg tcgtatgcgg cctgggcctg ggctaccgga
     3241 tgttcagcct cctgcaccgc ggtggcatac ttcaggtcga tggcgcgctt catttccgcc
     3301 cgttgttcct cgtgcatgta cacgagttcc tggcgtattt cgccgtccaa tagcaccagg
     3361 tccagcgcgg tgccgccgac cagggcaacg atcaatgcaa tgaacacgcg caaagcaccg
     3421 atcccccaag aacgatgcga aatctggatg atggaacgat ccacaatgaa gaccagcacc
     3481 gtgcagacca aggccaatag cacggtgtgc gtagtgccga gctgcagcac ttgagaaccg
     3541 atgaggcagc tcatggaggc ccaagtgacc accacggcga gcaggcccat ggccatcatg
     3601 gagatcttct tgcgggagtt cgacccgaat tggtccacgg tttgggcgcg gtccccggta
     3661 agcaggcaga agaagttgaa catggtcagc gggtgtattg gttgaggaac aggatctctt
     3721 ggaggtactt ctcgtagcct tgcttgaagc cggcatgaaa ggaagcgacc ggcagttcgg
     3781 caaagccgtc gttgcccacc accaggagct tgtgcccttc caaggtgagc cggtaccgct
     3841 ccatgtcttc gcgctgggtt tgcgtcttac ggatggtggc gctgtgcgcg ggctcatcgc
     3901 cgagagcact cagctcgcga tccaggttgg cgatcatgcc ttccagcttg tcgcactcct
     3961 cgttcaaggc caagcggatc tccgccttga tgcgctccac cagcttgcgc cccgtttcca
     4021 ggtcgtggaa gtcgaagccc gcactgtagc cctcctggaa gcggtcgcgg ttggccaatt
     4081 gcttcacccg cgacacataa gcaaccggtt gttgggcggg agcggacgat gccggagcag
     4141 ccgcaggcat ggggatctcg gtgtcgatga agatctcccg attgatgggg gcgctcacgg
     4201 gcgtggcaat agcagtggca ggagcggttc ggggcttgcg gctgatcagt tggatgaagc
     4261 ggttcatggc aaaagggttt cagggtcggg gtcagatcaa gtggctggga acggtgaatt
     4321 gttggtggcc ttggtagtgg catgcatcgc aaagcgtgat caggtaccgg tctggatagc
     4381 accaaggtgc cagcttgtac ccagtggcct tgtggcggtg gtattgccgg tgatgtacct
     4441 gcaggttacc ccgggtaccg caattgcggc attggtgggc atcacgggcc agcacgctcg
     4501 cgcgcttggc cttccacttg gggtgctgca gcatctcggc ataggtggcg ttatgggttt
     4561 gcgtggtgct catgtcctgt tcaggctgca agtttccggg gcgactgtgc agtctatctg
     4621 cactggtcag ttcccctccg atccctgcct gctgtatgtt cattgcgtgt cgaggtgcgt
     4681 tgcggtgcaa agcaggacca tatgtgtgca gcctttctgc acgggtagga gcctgttccg
     4741 cttttgcttg gggcatgttc atcgggtgcc gaggtgttgt gcagtgcaaa gcaggtgcgc
     4801 ttgtgtgcag tctttctgca caggttgttg tccgttctgc tcttgtcagg cgcatgcctt
     4861 ggtgcgagtt gcgttgcata gcagcaccac ctgtgtgcga tctttcctcg cgggacgctc
     4921 agatatacct cttttccagt aaggccgcga cggcgggaaa gcccgttatg cagccgagat
     4981 cacttcgtgt gtggcctcac agatacttcg catcactgcg gaatgcaaca gcatgatggt
     5041 atcccgcagg ttgctcattt cccctttggc gatcttgcgc aggatcacgg tttcgggtgg
     5101 atccgcaagt ccgggatcga ttcaaagtgc tttctgttca gcgtggctac cggcaggtcc
     5161 gcttccaacg ccgtggcggc tatgaacaga tcagggagtg tcacttgctt gcgctgtcgt
     5221 ttcaattcgg cttggatcgt tgcggcacgg cgcacttccc ttgatccgag tggcaacacc
     5281 tcaatgtgct caagcactcg gctccaatac gcgagctggc ccgtgtggca ccaacgtaca
     5341 cttcatattc cgtgatcacg gaaagtttca gggtgcaacc gtcctcagcc aattggtgga
     5401 gcaacgtccg gcctttgtcc tttttgcgga aatagtcgat cagcacggac gtgtccacaa
     5461 gcactactgg tcccgccatg ttgcgaaggc gttgcgggct tcctccatgc ggagtagttg
     5521 ctccttggaa aatacaggcc cgtgcaacag cagctcagcc aagctttctt cagcttccga
     5581 ggtcttgccg gccggcttcc gttcaagcac ggcttttgct ttccgccgct gggtaggtgt
     5641 caattgtgcc aaggcccgca gcagttgctc aaagggcatg ttcaacttga cctccatgtt
     5701 gtaaaggtaa caaaccaggt ccctatagtc atatcacacc cttcccccct tgatctcatc
     5761 cactacgttg gggtccaata gagtcgtcgt atcccccagg ttgctcatct ccccctcggc
     5821 gatcttgcgc aggatgcggc gcatgagctg gtgtggtgga gatattcact gcgattgaag
     5881 accccgaagg ggtcgcacag cgttgcattg aaggggagag tgtcacgacc ctgaaggggt
     5941 cgcacactgc ggcaggtatt tcggatcgta ttccacacca gcctcttcca ggaacgttat
     6001 cagctcttct tggaacgagc gatggcggtg atgttcttcc tggttgttga tgtaatgggc
     6061 caaaggttgg agctcccgcc agcccacagt gaacacggcg tagccgtcct gccatttgaa
     6121 ttgggccaca cggccgtttg tgcggatcca ttgggtggaa gccttcttca cttctcgcac
     6181 cgcatcggcc accgtttgcg ttgtcttgag gtccgccagg atatgcacat ggtccttcca
     6241 acctccgaca ttgcgggcat gaccgcccag accattgatc gtaccggcca tgtatgccca
     6301 cagtttctcc cgccaacgtt ggtccaagca agctacccgg tgttgtgtgg agaagacgat
     6361 gtggaagtgc atcttggaat aggatgacat ggccggtgga tttccgtgaa ggtatgcggt
     6421 tgtgatgtgc gaccccttca gggtcgttcg aatgtggtcg atctgacaac gttgtgcgac
     6481 cccttcaggg tcgaggaaag tgctggattg attgcaacgc tgtgggaccc cttcggggtc
     6541 caacttcgat cacgctcctt tcacaccctc ccccccttga tctcttccac caccccggga
     6601 tccagcagtg tggtggtgtc ccccaggttg ctcatttccc cctcggcgat cttgcgcagg
     6661 atgcggcgca tgatcttgcc gctgcgcgtc ttcggcaggc cgctcacgaa ctggatcttg
     6721 tccggcttgg cgatgcggcc gatggtggcc accacggatt cgatgatctc gccggtgatc
     6781 ttgtccacgg cttgcttgtc cttctggtcc agtggctcgg cagtgatcac gtaggcgtag
     6841 atgccctggc ccttgatgtc gtgcgggtag cccaccacgg cgctttccac cacgcgctcg
     6901 ctgaggttga tggcgctttc gatctcggcc gtgccgaagc ggtggccgct cacgttgatc
     6961 acgtcgtcca cgcggccgat cacgcgccag cggccgtcgg catcacgctt ggcgccgtcg
     7021 ccggtgaagt agtggcctgg gtagctgctg aaataggtct gcttgtaacg ctcgtgatcg
     7081 ccccaggtgg tgcgcaggat ccccggccac gggaatttta tgcagagcat gccctcctcc
     7141 ccgttcgctg tgatctcctt gccttcggcg gtgagcagca cgggctgtat acctggtaac
     7201 gggtaggccg cgtggcccgg cttctcatcg gtgacgcccg ccaaggagct gatgaggatg
     7261 ccggcggtct ccgtctgcca ccaggtgtcc acgatgggac agcggttctt gcccacgtgc
     7321 agcttgtacc actgccaggc ttcctcgttg atgggctcgc ccacgctacc gagcaccttg
     7381 agtgaatcca gcgagtaggc cagcacgtgg tccaacccgt gggccatcag gctgcggatg
     7441 gcggtgggcg cggtgtagaa gatgttgacg ccgtgcttgt cgatcacctg ccagaagcgg
     7501 cccgcgtcgg ggaaggtggg cacgccctcg aacatgagcg tggtggcccc ggcgcagagc
     7561 ggcccgtaga tgatgtagct gtggccggtg acccagccga tgtcggccgt acaccagtag
     7621 atatcgcttt cctcgtactg gaacacgttg cggaaggtgt agtcgcaata aaccatgtag
     7681 ccgccacagg tatgcaccac gcctttgggc ttgccggtgc tgccgctggt gtagaggatg
     7741 aagagcggat cctcggcgtc catctcttcg gcggggcagt gcttgtccac gtgcttcagc
     7801 tcgtcgtgca gccacacgtc gcggccctcc tgcatgtcca cggcccagcc taagcggtcg
     7861 gtgacgatga ccttcttcac gctggggcat tcctccagcg cctcgtccac cacgcgtttc
     7921 acggggatct gcttgtggcc gcggtgcagg ccgtcgctgg tgagcaccat gctgcaccgg
     7981 gcgtcgttca cgcggtcggc gatgctcttg gcgctgaagc ccgcgaagac caccgagtgg
     8041 atggccccga tgcgcgcgca ggccagtacg gcgatggtga gctcggggat catgggcatg
     8101 tagatgcaga tgcggtcgcc cttcttggcg ccgttgcgct tgagcacgtt ggcgtactgg
     8161 cacacctttt cgtgcagctc gcggtaggtg tagcggatga agcgttcgcc gggatcgttc
     8221 ggctcccaga tcagcgcgag cttgttgccg cgcttttcca ggtggcggtc caagcagttc
     8281 tctgtgatgt tgagcttgcc gccgatgaac cacttcacct cgggcttgtg gaagtcccac
     8341 tccagcacct tgtcccactt ctcgcgccag gtaaaggttt cggcctgtgc ggcccagaaa
     8401 gcttcggggt cttggacgct gaggcggtag gcctcgtcgt attcagcacg gctccgcagc
     8461 ctgcggaacg gcgtgtcctt gtgaatcgtg gtctccatgt tcagtccgtg atccattgag
     8521 cacggtcgct ccgttccgcg cattgcggaa tgatccgttt gcgaggcgat ccagttgcgc
     8581 cgtgcggcta aatgtcgaac aaaatccagt gggcacggac caggcctccg agcgctgttg
     8641 gaactacacc actctggatc gaggattgcc aaccggacag aggcaccacg tccagcgaac
     8701 cggcacaccc gggccgtgga agtcaacgca tcggaccgat ccggatcccg cctctgggtt
     8761 acgtgcatcc tcaccaccac cacggcgttg atatcgcgtg gatccgggcc gcgttgtacc
     8821 cggatgcagt ccgttggttt gccggaacaa ccgatgcacg cgggcagcac cctctccatg
     8881 atcgcattgg cagctacggc ggcagcggcc cagc
//
LOCUS       DAOQOC010000002         8710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_2991_length_8710_cov_3.914616, whole genome shotgun sequence.
ACCESSION   DAOQOC010000002 DAOQOC010000000
VERSION     DAOQOC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_8710_cov_3.914616"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1246)
                     /locus_tag="PL106_00055"
     CDS             complement(8..1246)
                     /locus_tag="PL106_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31483.1"
                     /translation="MMIILLRSISLLSLLAPYGCSVRAQVDLVLDQGFRSDVTVPLSE
                     LSKPSLEYPVLYGQLAVTMDFSASVTNLGAVPATDVFVILSVVHSTGTAGPFVSAVLP
                     MLAAGGSDTLSINAAFDGSWTEQELVLHFSVSSLEADIDPSNNSDSTSMWVTYSQCTS
                     RTQGESTGALANSGAPYMIGNRYEITEEIIPPVCALSCVVPYDPALIGSFITGYLFDE
                     SWNELSEGSEVLLTASNMSEPGETNRITLPLLSEVYLQTHHDYYAMVADWVGSEGIAI
                     ATEGISPDSSSWLIDIGSMTWSSIAQTPMVDIGIGYMGFCYSAIGEHQVDLQVVSPPF
                     PAPCASTVMIPVRLSGSTSIQWVIVDPLGRIVICPRSIHEAFGDLIIKIDINELSSGT
                     YFCILRAKDVTQQLQLLVGR"
     gene            complement(1260..2750)
                     /locus_tag="PL106_00060"
     CDS             complement(1260..2750)
                     /locus_tag="PL106_00060"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31484.1"
                     /translation="MEHYRLALLVMVLQGTSSSAQTFSTRNDFNNAQGPDEMGWTLVR
                     SDSGYLVIQACEDTMNMFLRVGLAHLSQNGTVSEIRSFGQDGLALYVGWANGTVRSPQ
                     GFALCGSTDDGIDNRAIVWQLDEELDSLWTLEPYEDTVDFSGGTMARSKNGVIAAVGG
                     VWHTGQSGRAFLALVDPSGILQNLTEYGTSMRDFAVSVDTCSDGGFVIGGDSWLTGTD
                     HDGFIIKTDAAGSQEWRRDLGGPYRDSYLSVISTSDGGYIAVGAYATYQSGFSTYYRL
                     YAARLNANGDLIWEKQYGQQGSVNELSSVTELHDGTFIAAGLYPSSGGDKGVLLKFAA
                     NGDSLWMRTFRHPPLDSVFSVHWLRHVVEDTDGSLVATGSCNDGQQDLWVLRVDSFGC
                     LVPGCQLYDHISEQPGTASKSELNFMLYPNPATGHVYISFRSAGRPKGEFRLYDPKGA
                     LVRRFAPTSRSEELEIALTQYPSGSYLICYVESIQGVRWAQTLIKE"
     gene            complement(2756..8323)
                     /locus_tag="PL106_00065"
     CDS             complement(2756..8323)
                     /locus_tag="PL106_00065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNA31485.1"
                     /translation="MCPAVDAQERWKAMLQTEGANYHDVKAIYEADMEGVDPAPDGDA
                     EEMQFKRFQWFWEGRTETGDPAKSGLLKNYGLKLSQYFANEICEENLNPTPWTNVGPD
                     KDPLRQHLGKVQCLWFDANQDPPMLIYAGSAHGGLWRSTDGGITWVNITDSFRQPVLG
                     VYSLARIEVGGNPRLLIGTGSWPADGYSLGVLWSDDDGITWNVSSGIPTVASLGTIVD
                     MQVYGNEVLAVQGTRAWRSTDHGESFTEITPTTANVIFGNVPDNSNRFTGIGHYLNGG
                     DGVIVIAAKDGDATGNGPDIYASEDLGQHWARITDQLPIAMGSGYAFRQYFPLNGSAT
                     SYYETKWVDWDIGQNNDDLWDFDGTTDIYGQDGTMFISPTHSIPWTTAAHYCDDNQPH
                     DGVCEDVIAGMNKPQYIQVKFVKDPDAEFNIYLTNDPSDLSTAQLVFSTELYPGVENN
                     TQMPVPTFIADDDFKNLVVEAKFLVPGYVPVVGIRIDEILMGSSLVAVSQVSVVNDPG
                     GAFDGFHLLTRSFGSTLNVKAHLYSGQTAGGINLHQLFDSNCDYEWPRDYNLGYDRFT
                     INKFGRVFASGVEGIHEMDVASGACTEVLLGYDDEQSPYWNLRHDDVRSFFIADGATA
                     GQERIAIGHDGGLAYRAVSGTWSDWNGESFPTVMLYDLGMDEFATRMLAGAQDNDTWE
                     WSMDPAQPWTNIYGHVDGGSANVLRNRDGSIDLIGYTENASRRFKGTYLGNTINLSHS
                     SDGCCFRLYWPSLISVASGEPRMYMAHSFNDTSPGVGAPGLYQVDPLHTGTNALNLTP
                     GPEHELIYNATGLDIHPANEDIIFVGIGGHYWGTPDQYRLIRTTDHGANWTDLTSALV
                     NPASTSSDQNVFSRYGVNTLTVDPDSDPNDIDHMTVYIGMDGIPDEDEFERVFKSIDG
                     GTTWTDMSAGLPGFPVTDIVVQPGSGGILYASTDIGVYVYEPLNNHWYCFNEGFPVCL
                     VTGLDLSACTGKLYASTYGRSIWSTDLYAPDRGDYFVVDTDLPLSGTHKITKNILVTA
                     GNTLTITGTLEFIPKRKLFIEPGAKVDVNGGILTGRCDQPWDGVEVWGVTTEDQSPST
                     NQGELLMRNGAILENATVGVRLFATIGESIGSDGDQDYIPGTTGGIAHMHSGSIIRNC
                     GRGVWFKPYKNMISAVEHVNRSHFLDAVFEKNGDAYDGMSYSDMARLEGVHGVLFSNA
                     VFDNMNGHSETPKMIGIRSLDTKFTASNCTFIKLGIGVRGTDATGLKPCTIRGSDFEN
                     NDRGVVLTGVFNAEITNNTFTIPSGDGSWPLDQSILPYPVPYGAYLIGCGGYEVEDNE
                     FNGVNSVGNAGLVIKNSDQVPDEFYRNEFHTLYVGSLLQGNNRLPGSQDGLRFRCNTF
                     GREDGTDDCVYDAAITLNSAAIAQNQGGPSDPIGNRFYPECPGGVGDESDVYHDDESL
                     EGIIYYHHTDQFTKPDCSTQPPKVIQQSTTFIFTPGDGSGSSCPADQSGDHLLSEVKA
                     TFQNGATDHAQLKALFDGELDGGDTEYLLEQIRDPLVSSFTLRNVMVASMPLKDLVLL
                     AAIDRDPAMDPWHLTEVLLAAGRLNPDVLVALMHSGIDQYFIDLVREAQNGTPVSWKQ
                     WLEAELSGLLHNTERARHDYVRKVLEDSTSNPLDSIDVIFNSEQLPSSPAERLSLLIV
                     ESQWQEVDEWISVPEEHGLTPHEAIVFGVVRDVAQDPMNAIGTVQDALEELNPIAEDE
                     NNSAHILARALLIRHLHMTFEEPIGLPGEERNMIEAFTNTDRPTKGLMRAKPNPVNER
                     LQVMTELPTREVAGYVVLYNVFGIELERTRISTGPQFFEIEVTKYAPGIYRLAMIGRN
                     GTLLDGLSISIVR"
     gene            8501..>8710
                     /locus_tag="PL106_00070"
     CDS             8501..>8710
                     /locus_tag="PL106_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA31486.1"
                     /translation="MTFLVIHLAPLGVAGQVIPWNFPLLMAAWKLAPALACGNTVVLK
                     PAETTPLTALKLAELLQEAELPDGVV"
BASE COUNT         1889 a         2500 c         2359 g         1962 t
ORIGIN      
        1 agaaaggtta tcgtcctaca agcaactgca attgctgcgt aacgtctttg gcgcgtagta
       61 tacagaaata tgtgccggag gatagttcgt tgatatcgat tttaatgatc agatcaccga
      121 atgcctcatg gatcgacctt ggacatatga cgatccggcc aagtgggtcg actatcaccc
      181 attggatcga tgttgaacca ctaagcctga ctgggatcat cactgttgat gcgcatggag
      241 cagggaaggg gggcgaaacc acttgtagat caacctgatg ttctccaatg gcggagtagc
      301 agaaacccat gtatccgatg ccaatatcta ccattggcgt ttgcgctatt gaagaccaag
      361 tcatcgatcc gatgtcgata agccacgaag aactatccgg actaattcct tctgtggcga
      421 tcgcgatgcc ctcactgccg acccaatccg ccaccatggc atagtagtca tgatgcgttt
      481 gcaagtagac ctctgaaagt agcgggagcg ttatccggtt ggtctcacct ggctcgctca
      541 tattgctggc ggtgagcaga acttccgagc cttctgaaag ttcattccaa ctttcatcga
      601 acaagtagcc agtaatgaag ctccctatga gtgcgggatc atagggcacc acacacgaca
      661 gtgcgcagac cggcggaatg atctcttcgg tgatttcata cctgttgcct atcatgtatg
      721 gtgcaccact gttagccagt gcccccgttg attctccttg tgtgcgtgaa gtgcattggg
      781 aataggttac ccacatcgac gtagaatcag aattgttcga tggatcgata tctgcttcaa
      841 gcgacgacac gctgaaatga aggacgagct cttgttcagt ccatgagcca tcgaatgcgg
      901 cattgatcga cagcgtgtcg ctgccgcctg ctgcaagcat tggcaacacc gcgctgacaa
      961 agggtcccgc agtgccggta ctatgaacta cggacaggat cacaaacacg tcggtcgcgg
     1021 gaacagcgcc aaggttggtc acgctggcgg aaaagtccat ggtaacggcc agttgcccgt
     1081 agagtactgg atattccaat gagggcttcg acaattcgct cagaggcacc gtaacatcac
     1141 tgcggaaccc ttgatcaagc acgagatcaa cctgtgcacg aaccgaacag ccatatggag
     1201 ctaacaggct cagcaaactt atactccgta gtagaatgat catcatggag ttcatttccc
     1261 tactctttga tcaaggtctg cgcccagcgc actccttgta tggactcaac atagcaaatg
     1321 agatatgatc cggaaggata ttgggtgagc gctatctcca gttcttcact gcggctggtc
     1381 ggagcgaacc ttcttacaag cgcccctttt ggatcataca ggcgaaattc gcctttgggt
     1441 cgcccggccg atcgaaagct gatgtataca tgtcctgtcg ccggattggg atagagcatg
     1501 aagttcagtt ccgacttgga ggcggtgcct ggctgctcgg agatgtggtc gtatagctgg
     1561 caccccggca ccaagcatcc gaagctatca acccgtagca cccaaagatc ttgttgaccg
     1621 tcgttacacg aacccgtggc tacaagactc ccatctgtat cctcaacaac atgccgaagc
     1681 cagtgaacgc tgaacacgct gtccagcggt gggtgtcgaa aggtgcgcat ccataggcta
     1741 tcaccattcg cagcgaactt caacaacaca cctttatcac caccagatga aggatagaga
     1801 cctgcggcaa tgaaggtgcc gtcatgcaat tcggttaccg acgagagttc attgaccgag
     1861 ccctgttgcc catactgctt ctcccagatc aaatcaccat tggcgttcaa acgcgctgcg
     1921 tacaagcggt agtaggtgct gaatccggac tgatatgtgg catatgcccc aacggcaatg
     1981 tatccgccat cactcgttga gattacgctc aggtagctat cccgatatgg accaccgaga
     2041 tcacgacgcc attcttgaga tccggcagca tcagttttta tgatgaaccc atcgtgatct
     2101 gttccggtga gccaggaatc gccgccgatg acaaatccac cgtccgagca tgtgtccact
     2161 gatacagcga agtcgcgcat ggatgtgccg tattccgtca agttctgcaa tatgccgctg
     2221 ggatcaacga gtgcaaggaa tgcccgtccg ctctgacccg tgtgccatac cccacccaca
     2281 gcggcgatca caccgttctt gctcctcgcc attgtgcccc ctgagaaatc aacggtgtct
     2341 tcatagggct ccaacgtcca aaggctgtcg agttcttcgt ccaattgcca tacgatggct
     2401 ctgttatcaa tgccatcgtc agtgctgccg cacagtgcaa aaccttgcgg tgatcgaacc
     2461 gtaccattcg cccagccaac ataaagggca agaccgtcct gcccaaaaga acgtatctcg
     2521 gacacggtac cgttctggct gaggtgagca aggcctacgc gtaggaacat gttcattgtg
     2581 tcctcacacg cctgtatcac caagtatccg ctgtcgctcc gcaccaaggt ccagcccatt
     2641 tcatcgggtc cctgtgcatt gttgaaatcg tttcgtgtgc tgaatgtctg cgctgacgaa
     2701 ctggtccctt ggagaaccat gaccagcagg gcaaggcgat aatgctccat tgaactcacc
     2761 gcacgatact tatggacaac ccatcaagta acgtcccgtt ccgtccgatc atggccaggc
     2821 gatagatccc gggggcgtac tttgtcactt cgatctcaaa gaactggggg ccggtactta
     2881 tccgtgtcct ttccagttca atgccgaaca cgttgtacaa caccacatat cctgcgactt
     2941 ctcttgttgg cagttcggtc attacttgca accgttcgtt gaccgggttg ggtttagcac
     3001 gcatcaagcc cttcgttggt cgatcggtgt tggtaaacgc ctcgatcatg ttgcgttcct
     3061 cacccggcag cccaatgggc tcctcaaagg tcatatggag gtggcgaata agcagtgcgc
     3121 gggccagtat gtgcgcggag ttgttctcgt cctcagctat cgggttcaac tcctcaagtg
     3181 catcctgcac cgtgccgatc gcattcatgg gatcttgggc aacgtcgcgc accaccccga
     3241 atacaatagc ttcgtgggga gttagtccat gttcttcggg tacacttatc cactcgtcca
     3301 cttcctgcca ctggctctcg acaatcaaca gcgatagccg ctcggcaggg ctgctcggta
     3361 gttgctcact attgaagatg acgtctatac tgtccaatgg attgcttgtt gaatcctcca
     3421 gtaccttacg cacatagtca tgtcgtgcgc gctcagtgtt gtgcaacagc ccactcaatt
     3481 cggcctccag ccattgtttc cagctgaccg gcgtgccgtt ctgcgcttct cgtacaaggt
     3541 cgatgaagta ctgatcgatg ccactgtgca tgagcgcgac caatacgtca gggttcaacc
     3601 ttcctgcggc aagaagcact tctgtcaaat gccatggatc catggcagga tcgcgatcga
     3661 tcgccgccag taggaccaga tccttcagtg gcattgaagc caccatcacg ttgcgaaggg
     3721 tgaaagagct taccaacggg tcgcggatct gttcgagcag gtattctgta tcgccaccat
     3781 cgagttcacc atcgaagagc gctttcaact gtgcgtggtc ggtagcaccg ttctgaaagg
     3841 tcgccttcac ttcggataac agatgatcgc cgctttgatc ggccgggcac gatgaacccg
     3901 atccatcacc cggggtgaaa atgaaggtgg ttgattgttg gatcacctta ggtggttgcg
     3961 tcgaacaatc gggtttggtg aattgatcgg tgtggtggta atagatgatc ccttctagcg
     4021 attcgtcatc gtggtacacg tcgctctcat cgccaacccc acccggacat tccggataga
     4081 agcgattgcc aatggggtcg ctgggacctc cctggttctg agcgatggcg gcagagttca
     4141 gggtaatggc ggcgtcgtag acgcaatcat cggtgccatc ctcgcgaccg aatgtattgc
     4201 aacggaaccg aaggccatcc tggctgcccg gcagacgatt gttcccctgc aacaagctgc
     4261 ccacatacag cgtgtggaat tcgttccggt agaattcgtc aggcacttgg tcgctattct
     4321 tgatcaccaa gccggcattg cccacgctat tcaccccgtt aaactcattg tcctccacct
     4381 cgtagccgcc gcagccaata agatacgcgc catagggcac ggggtaggga aggatggact
     4441 ggtcaagggg ccatgatccg tccccactgg gaattgtgaa agtgttgttc gtgatctcgg
     4501 cattgaacac gccggtaagc accacgcctc ggtcgttgtt ttcgaaatca gagccgcgga
     4561 tcgtgcaggg cttcagcccc gtagcgtcgg tgccgcgcac tccgatgccc agtttgatga
     4621 aggtgcaatt ggaggccgtg aacttcgtat cgagcgagcg gatgccgatc atcttcggcg
     4681 tttcggaatg cccgttcatg ttgtcgaaca cagcgttcga gaacagcact ccatgcacac
     4741 cttcaagtcg ggccatgtct gaatagctca tgccatcgta cgcgtcaccg ttcttttcga
     4801 aaacggcatc caggaaatgg ctcctattga catgttccac ggcgcttatc atgttcttat
     4861 agggcttgaa ccaaacgcct cggccacaat tgcggatgat gcttccggaa tgcatgtgcg
     4921 cgatgccccc ggtggtcccg ggtatgtagt cctggtcgcc atcgcttcca atggattcac
     4981 cgatggtcgc gaagaggcgc acgcctactg tggcgttctc cagtatggcg ccgttcctca
     5041 tcagcagttc gccttggttg gtcgaaggac tctgatcctc cgtcgtgacc ccccacacct
     5101 cgacgccatc ccagggctga tcgcatcgac cagtaaggat gccaccgttc acatcgacct
     5161 tcgccccggg ctcgatgaag agctttcgct tcgggatgaa ttccagcgtg ccggtgatgg
     5221 tcagggtatt gcccgccgtg accaggatgt tcttggtgat cttgtgcgtg ccgctcaacg
     5281 gaaggtccgt gtcgaccacg aagtaatctc cacgatccgg tgcgtatagg tcagtgctcc
     5341 aaatggacct tccgtaggtg gaagcataca acttgccggt gcaagcgctc aggtcaagcc
     5401 cggtgacgag gcacacggga aatccctcgt tgaaacaata ccagtggttg ttcagcggct
     5461 cgtacacata gacgccaatg tccgtcgacg cgtacaggat gcccccgctg ccgggctgga
     5521 ccacgatatc agtaaccggg aatccgggca gtccggcgga catgtccgtc caagtcgtgc
     5581 cgccatcgat gctcttgaac acgcgttcga actcgtcctc gtccgggatc ccgtccatac
     5641 cgatgtacac cgtcatgtgg tcgatgtcgt tcgggtcgga gtccgggtct accgtcaacg
     5701 tgttcacacc gtaccgtgag aacacgtttt gatctgagga tgtactggcc ggattgacca
     5761 gggcggaagt aagatccgtc caattcgcac catgatcggt cgttctgatc aagcggtact
     5821 gatccggagt accccaataa tgacctccga tgccaacgaa gatgatatcc tcattggccg
     5881 gatggatgtc cagcccggtc gcattgtaga tcagctcatg ttcgggaccc ggggtcaggt
     5941 tgagagcgtt agtacctgta tgcagcggat cgacctgata gagacccggt gcacccaccc
     6001 cgggggaggt gtcattgaac gagtgggcca tgtacatccg gggttcaccg gaagccacgc
     6061 tgatcaaact cggccagtaa agccggaaac aacagccatc cgaagaatgg gagaggttga
     6121 tcgtgttgcc tagataggtt cccttgaaac gccgcgacgc gttctcggta tacccgatca
     6181 gatcgatgga tccatcgcgg ttgcgcagca cgttcgcgct ccctccatca acgtggccat
     6241 agatgttggt ccagggctgg gcaggatcca tgctccattc ccaggtgtca ttgtcctggg
     6301 caccggcgag catgcgggtc gcgaactcgt ccattcccag gtcatagagc atcacggtgg
     6361 ggaacgactc cccgttccaa tcgctccagg tcccgctgac ggctcggtaa gcgagcccac
     6421 catcgtggcc gatcgcgatc cgttcctgtc cggcggtggc accatcggct atgaagaagg
     6481 agcgtacgtc gtcatgcctc aggttccaat agggggattg ttcgtcatca taacccaaga
     6541 gtacctcggt acatgcaccg gacgcgacgt ccatctcgtg gatgccctcc acgcccgagg
     6601 cgaaaacacg accgaacttg ttgatcgtga accggtcata acccaggtta tagtccctgg
     6661 gccactcgta atcgcaattg gagtcgaaca actggtgcag atttatccca ccggcggttt
     6721 ggccgctgta gagatgcgcc ttaacgttca gcgtgctgcc aaaggagcgt gtgagcaaat
     6781 ggaagccgtc gaaggcgccg cccggatcgt tcaccacact gacctgcgat acggccacca
     6841 ggctgctgcc catcaagatc tcatcgatgc ggatcccgac caccggaaca tagcccggca
     6901 ccaggaactt cgcttccacc acaaggttct tgaagtcgtc atccgcgatg aaagttggca
     6961 ccggcatctg ggtgttgttc tccacgcctg ggtagagttc ggtgctgaac accagttgcg
     7021 cggtgctcag gtcgctcgga tcgttggtga gatagatgtt gaactcggca tcgggatcct
     7081 tcacgaactt gacctggatg tattgcggtt tgttcatccc ggcgatcaca tcctcacaaa
     7141 cgccgtcatg gggttggttg tcatcacaat agtgggcggc ggtggtccaa ggaatggagt
     7201 gcgtcggcga gatgaacatg gtcccatcct gtccatagat gtccgtggtc ccatcgaaat
     7261 cccataggtc atcgttgttc tggccgatgt cccagtccac ccatttggtc tcataatagc
     7321 tcgtcgcgct tccgttgagc gggaaatact ggcggaacgc atagcccgat cccatcgcga
     7381 tgggcagctg atcggtgatg cgggcccaat gctggccaag atcctccgag gcatagatat
     7441 cgggaccatt gccggtcgca tcgccatcct tggcggcgat gacgatcacg ccgtcaccac
     7501 cgttgaggta atggccgatg ccggtgaagc ggttcgaatt atcaggaacg ttgccaaaga
     7561 tcacgttcgc cgtggttggg gtgatctcgg tgaaactctc accatggtca gtggaacgcc
     7621 atgcgcgtgt tccttgaacg gccagcacct cgttgccata gacctgcatg tccacgatgg
     7681 tgccgagcga ggccaccgtc ggtatgccgg atgaaacgtt ccaggtgata ccgtcatcat
     7741 cgctccagag cacacctagg gagtagccat cggccggcca gctgcccgta ccgatgagca
     7801 accgcggatt gccgccgacc tcgatccgcg caagcgaata gacacccagc actggctggc
     7861 gaaagctatc ggtgatattc acccaggtga taccgccgtc agtgctccgc cacaggccgc
     7921 catgcgcgct gccggcatag atgagcattg gcggatcctg gttcgcgtcg aaccagaggc
     7981 actgaacctt ccccaagtgc tgtcgcagag gatccttgtc gggcccaacg ttggtccaag
     8041 gcgttgggtt caggttctct tcgcagatct cgttggcgaa atattgcgag agcttcagtc
     8101 cgtagttctt gagcagcccc gacttcgccg gatcacccgt ttccgtacga ccctcccaga
     8161 accattggaa acgtttgaac tgcatctctt ccgcgtcacc atcgggcgcc ggatccactc
     8221 cttccatgtc cgcctcgtag atggccttca catcatggta gttcgcgccc tcggtctgga
     8281 gcatcgcttt ccaacgctcc tgggcatcaa cggctgggca catcaggaga gcgaaggctg
     8341 cgagtccggg aattcgatgt tgcacggcag tgtaattttc gtgtcagatg cgtggcccgt
     8401 ggatcaagga tccgtagcag gatgaaaccc tgatcaaatg taacgctgta gcgaggtcaa
     8461 tcccacagta tcgttaatca agtagaactc gctttgtttg gtgaccttcc tggtaattca
     8521 cctagcgccg ctcggcgtcg ccggacaggt catcccctgg aacttcccgt tgctcatggc
     8581 cgcgtggaag ctcgcccccg cgctcgcctg cggcaacacc gtggtgctga aacccgccga
     8641 gaccacaccg ctcaccgcgc tcaagctggc agaactgctg caggaagccg agctccccga
     8701 tggcgtcgtc
//
LOCUS       DAOQOC010000003         8297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3181_length_8297_cov_3.393836, whole genome shotgun sequence.
ACCESSION   DAOQOC010000003 DAOQOC010000000
VERSION     DAOQOC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_8297_cov_3.393836"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="PL106_00075"
     CDS             <1..1551
                     /locus_tag="PL106_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31487.1"
                     /translation="AASLDLNQWHMFVVGAETNPGSNNSWFTKGTSTQPNYGLWSPAN
                     GALIMPIYDILNNLNAPSAPAGTTDANFNVIEYSNRVLFILFPSRTLYKNATSVYTDA
                     SLLQLPANNGSQLYIGDTQDNTGWNLDGSIAEMIFYNGPLNAAQRIIVDNYLAAKYGR
                     TLTASDIYVQDNPANGNYDHDVAGIGRVNASNIHNDSRGSGIVQINAPSGLGNNEFLF
                     WGHDNQPLDAWGVTDFPPSVQGRLARVWRPTEANSAGTAVDVGSVNMTWDLTGLGSVT
                     AADLRLLVDSDNDGVFADETPIGPPTWVGGNLYRFTNLTALANNLRFTLATVDPVNTP
                     LPIELVSFTAEAIGSDAVELEWTTASEHDNDHFTIERSSGTGDWIPVLQVDAVGTSTA
                     TNTYTAVDDRITHELVYYRLRQTDLDGTSTVSEVVAVRMPLPAVQPIVYPNPSDGPIV
                     FVLPDGPESAMNFSLIDASGRVLPLNAQMLAPGRYLLDPGTLAPGAYVLRAQRPLSDE
                     FYSVRILR"
     gene            1874..2896
                     /locus_tag="PL106_00080"
     CDS             1874..2896
                     /locus_tag="PL106_00080"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNA31488.1"
                     /translation="MYALLRSLLFRLSPEKAHQLTFSLLELPGATLLSGGTPPPKEAA
                     LEVMGLRFPGPVGLAAGMDKDAQHVDALMRIGFGFVEVGTLTPVAQPGNDRPRLFRLK
                     PDHALINRMGFNNGGVQAAVQRLRKRAPGTIVGGNIGKNKVTPNEEAIRDYITCFEAL
                     HPVVDYFVVNVSSPNTPGLRALQEKGPLLAILKELRTRDGAKSVHRPILLKIAPDLTT
                     EQLDDIISVVKESGIAGVIATNTTIARAGLRTPKAVVEAMGAGGLSGVPVRQRSTEVV
                     KYLRGGLPKPFVIIGVGGIDSAEAALEKLDAGADLVQVYTGLVYEGPALLKRINQAFV
                     RWRSQQ"
     gene            2878..3744
                     /locus_tag="PL106_00085"
     CDS             2878..3744
                     /locus_tag="PL106_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNA31489.1"
                     /translation="MEKPAVKLIECPRDAMQGIHTFIPTDVKVKYLDGLLRVGFDTID
                     IGSFVSPKAIPQLADTAEVLSRIDLSSTRSKLLVIVANERGAEQACTQEVVHYLGYPF
                     SISETFQQRNTNTSIEGSWARTAHIADLARKVDKELVVYISMAFGNPYGDPWNAEVAL
                     HWTDRLVHELGVRIISLADTVGVAKPTDIGAMFTALIPALPHVEFGAHLHARKDNWKP
                     KTDAAWNAGCRRFDHAIAGYGGCPMAEDDLVGNLTTELFVQDLEARGIRSGLGLEQLG
                     RSVQEAGGVFPE"
     gene            complement(3751..5013)
                     /locus_tag="PL106_00090"
     CDS             complement(3751..5013)
                     /locus_tag="PL106_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA31490.1"
                     /translation="MSSRGLLNFSLVLAILVLIDLYAWKGINTALANHSQVIRRLVRL
                     LYWSISIGILVSLAWTLITMQDMRTRRDHSYVFSLIGITFLFLLPKLIIVIFHGLDDL
                     LHLVRLVWFKLMPGEPVGDAAIGEVIGRARFLSQLGLVLSSLPFAGVLYGMTKGRRTF
                     NVARMPVLSPKLPAAFEGLRVVQISDLHLGSYGGDLSIVRHGLELIQAEDPDLIVFTG
                     DLVNDFADEAEPFIDLMRALKAPMGKFSILGNHDYSDYARWDDPAAKAANLERLKAIH
                     GEMGFTLLLDEHRSLERNGDRIGLLGVQNWGVRFQQYGDLARTVQGTEGLPYRILLSH
                     DPTHWDAQVRATGIDLMLSGHTHGAQFGVKVGGTTYSPAQWVYKQWAGLYREGAQQLY
                     VNRGFGFIGFPGRVGMPPEITVLELRRA"
     gene            complement(5072..5983)
                     /locus_tag="PL106_00095"
     CDS             complement(5072..5983)
                     /locus_tag="PL106_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31491.1"
                     /translation="MGTTTLLLFLCIPNAFYFFIPYTESLFFAFAVLTIIGLLDRSAW
                     KVAVGLFLCSVTRPTFTTLLPAILLAVHFTGPGLNRDWRTATLCIVSCLMGIFFVSMT
                     QHADTGEWLGFYHAQSGWGNKLRLPTFPLRSWGGNDVVRLDAVAMLIGSAGAAPLFIT
                     GMRWPEDPSLPTRVVLLSFFSLFMVTMLVLVLRGGELFSLNRFIFATPFFLVMLNSVQ
                     GSVSITGWRWLWLFLVLEAYFLMFGSFVHIQTFLWFSAVVGFLMIVVAATMVRPMVQW
                     AKPLAILIAFIVQIGFTVKFLLGGWVA"
     gene            6572..7822
                     /locus_tag="PL106_00100"
     CDS             6572..7822
                     /locus_tag="PL106_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31492.1"
                     /translation="MLKIDEMGLLQWTAVIGDNGVQSGMACRVLEDGYVLAGTTSTGV
                     FGGYDVYVVRLDLNGQVLWEQSYGTTEWDLGKDIEVVDDGFVVVGTSYGSGSADALVL
                     RIDADGEEVWRSSIGDIGEDEGNGVIVTSGGEFVVTGAMANVEDDDRTDAFVSKLSVN
                     GDVQWTEIFGGDSADYFYSAVEMPDGGFACSGGTGSYSDVKQILLVKVDADGGQLWQE
                     VFGSMGDSEAREIQLNLNGDLAMASYNSYNNAGGKDMVLFIATAAGSFMLGKNYGGID
                     DEDGFSIQCTADGGYILVGIAEGYGPGITSIYVVKAAANGETVDDTVYEMFDASAVEA
                     PEATQLPFILSPDPASSFTRLATRTPITSATLFDSQGRLVRTWSAPVPQELDLRGLAE
                     GTYQFVAIGDDGRRMALPLMIAGN"
     gene            7826..>8297
                     /locus_tag="PL106_00105"
     CDS             7826..>8297
                     /locus_tag="PL106_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31493.1"
                     /translation="MNERPFRLTLIVLGHGVFLALLVLAWKHALLRSTFGDTAFQVFK
                     WVSQPGLDIEAHRYTAAVPQICVKAAMYFGPSLSTLLIIASLAHVLVGYGVFLLCAHG
                     WRAPVSALACAMATVLCTRLTFYSPVLEANYLLCLPFLLLGYLEARARALAPAHA"
BASE COUNT         1737 a         2401 c         2566 g         1593 t
ORIGIN      
        1 gctgcctcgc tcgatctgaa ccagtggcac atgttcgttg ttggtgccga gacgaatccc
       61 ggcagcaaca acagctggtt caccaaaggc accagtacac aacccaacta tggcctgtgg
      121 agtcccgcca acggcgcgct catcatgccg atctacgata tcctgaacaa tctgaacgcc
      181 ccgagcgccc cggcggggac caccgacgcg aacttcaacg tgatcgaata cagcaatcgg
      241 gtcctcttca tcttgttccc atcgcgcacg ttgtacaaga acgccaccag cgtgtacacg
      301 gatgcgagcc tgcttcagct gccggccaac aacgggagtc agttgtacat cggagatacg
      361 caggataaca cgggctggaa cctcgatggg tcgatcgcgg aaatgatctt ctacaacggc
      421 ccgctcaacg ccgcccagcg gatcatcgtg gacaattacc tcgcagcgaa atatggccgt
      481 acactcacag cgtcggacat ttacgtgcaa gacaaccccg cgaacggcaa ctacgaccat
      541 gatgtggccg gcatcggtcg tgtgaacgca tcgaacatcc acaatgattc caggggctcc
      601 ggcatcgttc agatcaatgc cccgagcggg ctcggcaaca atgagttcct cttctgggga
      661 cacgacaacc agcctcttga tgcatggggc gttacggact tcccaccgag cgtgcagggc
      721 cgcctggccc gtgtatggcg cccgaccgag gcgaacagtg ccggcacagc ggttgatgtg
      781 ggttcggtga atatgacatg ggacctcacg ggcttgggaa gcgttaccgc agcggatctg
      841 cgcttgctgg tggattcgga caacgatggc gtgttcgcgg atgaaactcc gatcgggccg
      901 ccgacctggg tgggcgggaa tctctatcgc ttcacgaacc tgacagccct cgcgaacaac
      961 ttgcgcttca ccctggctac cgttgatccg gtgaataccc cgctgccgat cgaactcgtt
     1021 tcgttcaccg ccgaggcgat cgggtcagat gcggtggaat tggaatggac cacggcgagt
     1081 gagcacgaca acgatcactt caccatcgag cggtcgagcg gtacgggcga ctggatcccc
     1141 gtgctgcagg tagatgccgt gggtacttcc accgccacca acacgtatac ggcggtggac
     1201 gaccggatca cccatgagct tgtgtactac cgtttgcggc agaccgacct ggacggaacc
     1261 agtacagtga gtgaagttgt cgcggttcgc atgccccttc cggcggttca accgatcgtg
     1321 taccccaacc ccagcgatgg tccgatcgtg ttcgtgttgc cggatggacc ggagagtgcg
     1381 atgaacttct cgctcatcga tgcgagtggc cgtgtcctgc cactgaacgc acagatgctc
     1441 gcaccgggcc gctatctgtt ggatccgggg accctcgctc cgggcgccta tgtcttgcgc
     1501 gctcagcggc ccctgtcgga tgagttctac agcgtgcgca tcttgcgctg agcaaggccg
     1561 gccttggctc gcatgtgggg tgctccgtcc cgcttgaacg aatgatcagt cctgatccgg
     1621 gtggtacgcg ttctgctttt cgacctgata ctcgctgcgc taaagccgga tggctcattg
     1681 agaagttgag cgtcctgcca gtctcatcgt atttggatct ctcgtggcac actcgaccct
     1741 ggagggtgca gggcgctgta catgtatcag cagcgaacac acgtaggaca ccgcagttcc
     1801 tcggtcagta ggagagacgg tgccggatac ccccgcttcc tcccatcttc aacgccggta
     1861 ccttcgcccg ccgatgtacg cgctgcttcg atcgttgctc ttccgccttt cacccgaaaa
     1921 ggcacaccaa ctcacattct ccctgcttga attgcccggt gcaacgctgc tgagcggagg
     1981 tacaccgccg ccgaaggaag cggccttgga agtgatggga ctgcgattcc ctggccctgt
     2041 gggactggcc gccggcatgg acaaggacgc ccagcatgtg gatgccttga tgcgcatcgg
     2101 cttcggcttc gtggaggtgg gcacgctcac gcccgtggcc cagccaggca acgaccgtcc
     2161 gcgactcttc cggctgaaac cggaccacgc gctcatcaac cggatgggat tcaataacgg
     2221 cggcgtgcag gctgcggtcc aacggctccg gaaacgtgcg cccggtacca tagtgggtgg
     2281 caacatcggg aagaacaagg tcacacccaa cgaggaagcg atccgcgatt acatcacgtg
     2341 tttcgaggcc ctgcaccccg tggtggacta cttcgtggtg aatgtgagca gccccaacac
     2401 cccgggtctt cgggcgctgc aggagaaggg ccccctgctc gcgatcctga aggaacttcg
     2461 tacacgtgat ggtgcgaagt ccgtgcatcg gcccatcctg ctgaagatcg cgccggacct
     2521 caccacagag cagctcgacg acatcatctc ggtggtgaag gagagcggca tcgcgggcgt
     2581 gatcgccacc aacaccacca ttgcacgggc cgggttgcgc acacccaagg ccgtagtgga
     2641 ggccatggga gcgggcggat tgagcggcgt gcccgtgcgg cagcgcagca ccgaggtggt
     2701 gaagtacctg cgtggggggc tgcccaagcc cttcgtcatc atcggcgtgg gcggtatcga
     2761 cagcgccgag gccgcgttgg aaaaacttga cgccggtgcc gacctggtgc aggtgtacac
     2821 gggtctggtg tacgaaggcc ctgcgctatt gaagcggatc aatcaagcgt tcgtccgatg
     2881 gagaagccag cagtgaaact gatcgaatgt ccgcgcgatg ccatgcaggg catccacaca
     2941 ttcataccca ccgacgtgaa ggtgaagtac ctcgacggat tgttgcgcgt gggcttcgac
     3001 accatcgaca tcggcagttt tgtgagtccg aaggccatac cacagctggc ggacacggcc
     3061 gaggtgcttt cgcggatcga cctttccagt acccgctcga agctcctggt gatcgtggcc
     3121 aacgagcgcg gtgcggagca ggcctgcaca caggaggtcg tacactacct gggttatccg
     3181 ttcagcatca gcgagacctt ccagcagcgc aacacgaaca cgagcatcga aggctcgtgg
     3241 gcgcgtacgg cacacatcgc cgatctggcg cgcaaagtgg acaaggaact ggtggtgtac
     3301 atcagcatgg ccttcggcaa tccctacggc gatccgtgga acgcggaggt ggccttgcac
     3361 tggacggacc ggctcgtgca cgaactgggc gtccgcatca tttccctggc cgatacggtg
     3421 ggagtagcca agccgaccga tatcggcgcg atgttcacgg cactcattcc ggccttgccc
     3481 cacgtggagt tcggtgcgca cctgcacgcc cgcaaggaca attggaagcc caagaccgat
     3541 gccgcttgga acgcaggttg ccgccgcttc gaccatgcca ttgccgggta cggcggctgc
     3601 cccatggccg aggacgacct ggtgggcaac ctcaccaccg aactgttcgt gcaggacctc
     3661 gaagcacgtg gcatccgctc cgggctgggt ctggagcagc ttggtcggag tgtgcaggag
     3721 gcgggtgggg tttttccgga gtgaccctgt tcaagcgcgg cgcaattcca gcaccgtgat
     3781 ctccggcggc atgcccaccc gccctgggaa gccgatgaag ccgaaaccgc gattgacgta
     3841 caattgttgg gctccttccc ggtagaggcc ggcccattgc ttgtacaccc attgcgcagg
     3901 gctgtaggtg gttccaccca ccttcacacc gaattgtgcg ccgtgggtat ggccactcag
     3961 catcagatcg atcccggtgg cgcgcacctg cgcatcccag tgggtgggat catggctcag
     4021 caggatccgg tagggcaggc cctcggtgcc ttgcacggtg cgggccaggt cgccgtactg
     4081 ctggaagcga acgccccagt tctgtacgcc caacagaccg atgcgatccc cgttgcgttc
     4141 caacgagcga tgctcatcca gcagcagtgt gaaacccatt tcaccgtgga tcgctttcaa
     4201 tcgctccagg ttggcagcct tggcggccgg gtcgtcccag cgtgcgtagt cgctgtagtc
     4261 gtggttgccc agtatgctga acttgcccat cggtgccttc agtgcgcgca tcagatcgat
     4321 gaagggttcg gcctcatcgg cgaagtcgtt caccaggtcc ccggtgaaga cgatcaggtc
     4381 cggatcctcg gcctggatca gctccaagcc atgacgtacg atgctgagat caccaccgta
     4441 actgcccagg tgcagatcac tgatctgtac cacgcgcagt ccttcgaagg ccgcaggcag
     4501 cttgggggag agcacgggca tacgcgccac attgaacgtg cgacgacctt tggtcatgcc
     4561 atagagcacg cccgcgaagg gaagcgagga cagaacaagc cccaactggc tgaggaagcg
     4621 agcgcggccg atcacctcgc caattgcggc atcacccacg ggttcgcccg gcatcagctt
     4681 gaaccagacg aggcggacca gatgcaagag gtcgtccaga ccatggaaga tcacgatgat
     4741 caacttgggc agcaggaaca ggaaggtgat cccgatgagt gagaacacat agctatggtc
     4801 ccgcctggtg cgcatgtcct gcatggtgat gagcgtccat gccaaagaga ccaggatgcc
     4861 gatcgaaata ctccagtaga gcaagcgcac caaccggcgg atcacctgcg agtggttcgc
     4921 caacgccgtg ttgatgccct tccaggcgta gaggtcgatg aggaccagga tggccaggac
     4981 caaggagaag ttgagcaggc cgcgtgagga catggggcgg attcgtgtgg ccaaagttgc
     5041 gtccaggctg gccgagtatg cacaccgttg ttcacgccac ccagcctccc agcaagaatt
     5101 tcacggtgaa accaatttgg acgatgaaag cgatcaagat agccagtggt ttagcccatt
     5161 gaaccatggg acgcaccatg gtagccgcga ctacgatcat caggaatccc accacggccg
     5221 agaaccatag aaatgtctgt atatgtacga atgatccgaa catcagaaag tatgcttcca
     5281 gaaccaagaa cagccataac caccgccaac cggtgatcga cactgacccc tgaacggagt
     5341 tcagcatgac gaggaagaag ggcgtcgcga agatgaatcg gttcaggctg aaaagttcgc
     5401 cacctcgcag cacaagcacg agcatggtca ccatgaacaa gctgaaaaag gaaagcagta
     5461 ctacacgcgt cggaagtgat ggatcttccg gccaacgcat gccagttatg aataacgggg
     5521 cggccccggc cgagccgatc agcatcgcca cggcatctag tcgtactacg tcgttgccgc
     5581 cccatgaccg tagcgggaat gtcggcagtc gcagtttgtt gccccagccg gattgggcgt
     5641 gatagaatcc gagccattca ccggtgtccg catgttgggt catagaaacg aaaaagatac
     5701 ccatcagaca tgaaacgatg caaagcgttg cggtacgcca gtcgcggttc aagccgggac
     5761 cggtaaaatg gacggccaag aggatcgcgg ggaggagggt cgtgaaggtg ggtcttgtga
     5821 ccgagcaaag gaacaggcca acagcgacct tccaggcgga tcggtccaac aagccgatga
     5881 tggtcagtac agcaaatgcg aagaacagtg attcggtgta gggtatgaag aagtagaacg
     5941 cattgggtat gcagaggaac agcaagagcg ttgtcgtgcc cacacggatc cgccatgcga
     6001 gccacgtgag ggcggctatg tacgtcaggc cattcacgat gcagatggct ggtatgctac
     6061 cccccaagac ctgccagagt ttcggcatca acgggaaaaa ggcctgccgg aagggaacgt
     6121 aacctcctga agcgatcgcg gcgtagtgct ccgcgtcaaa gtgcatcaag tcggaccacg
     6181 aaacaggcat cccggtcatg tgccaatgga ccatttggac tgataggaac gcaagggtgg
     6241 tgtggcctaa caccaaggca gcgaccttcc atggtcgacc cgtccatgtg ctatgggcct
     6301 ccgcgtttga catgcgccca aagatgcgac ctgcgatgcc acaacaaccc tgctaacttt
     6361 cccggccatg aacacttggt attctgccat ccgccttctc accacgtcgg ctctgaccgt
     6421 cttgatcgct gacgccgatg cccagatcac atggcgtcgg acctatggag gtttcgatac
     6481 gaataggggg atgtccgtgc gggtggctag tgacggaggc tatgtcatag ctggctctac
     6541 gggcagcttt ggagcgggtg ggcaagcgta tgtgctgaag atcgacgaga tgggactcct
     6601 gcaatggacg gccgtcatag gggataatgg tgtgcagagc ggaatggcct gtagggtgtt
     6661 ggaagatggt tatgtgttag ccggcaccac gtccactggg gtattcggtg ggtatgatgt
     6721 atatgtggtc cgattggatc ttaacggcca agtgctttgg gagcagagtt acggcactac
     6781 cgaatgggac ttgggcaagg acattgaggt cgttgatgac ggcttcgtag tggtgggaac
     6841 ctcgtatggc tcaggaagcg cggatgcgtt ggtgctgcgg atcgatgccg atggggagga
     6901 ggtctggcgg agttccattg gcgatatcgg agaggacgag gggaatgggg tgatcgttac
     6961 gagcggagga gaattcgttg taacgggtgc aatggccaac gttgaggatg acgatcggac
     7021 ggatgcgttc gtttcaaaac tgagcgtgaa tggtgacgtt cagtggacgg agatattcgg
     7081 aggagacagc gcagactatt tctacagcgc ggtggaaatg ccggatggtg gtttcgcatg
     7141 cagtgggggg accggcagtt acagcgacgt gaagcagata ctccttgtca aggtggatgc
     7201 ggacggggga cagctttggc aggaggtgtt cggcagtatg ggtgattctg aagcgcggga
     7261 gatccaactg aatctgaacg gtgatctggc gatggcctct tacaacagtt acaacaatgc
     7321 cggaggcaag gatatggtcc ttttcatcgc cacggctgct ggttcattca tgttgggtaa
     7381 gaactatggc ggaatagatg atgaagatgg tttttccatc caatgcacgg cagatggtgg
     7441 atacatcctt gttggtatag cggagggata tgggcctggg ataacctcga tctacgtcgt
     7501 caaagcagct gctaacggcg aaacagtgga tgacacggta tatgagatgt tcgatgcttc
     7561 tgcggtcgag gcacccgagg ctacgcaatt gccttttatc ctctctcctg atccagcatc
     7621 ttcattcacc cgcctcgcca cccgcacccc catcacctcc gccaccctct tcgactccca
     7681 aggccgcctc gtgcgcacct ggtccgcccc ggtaccgcag gagctggatc tgcgcggcct
     7741 ggccgaaggc acctaccagt tcgtggccat tggcgatgat ggccgccgca tggccctgcc
     7801 gctgatgatc gcggggaact gagcgatgaa cgagcggccc ttccgcctca ccctgatcgt
     7861 gctgggccac ggcgtgttct tggcgttgct cgtgttggca tggaagcacg ccctgttgcg
     7921 ttccaccttc ggcgataccg cttttcaggt gttcaagtgg gtaagccagc cgggcctgga
     7981 catcgaggcg catcgttaca cggccgccgt gccacagatc tgcgtaaagg ccgcgatgta
     8041 cttcggacca tcgttgagca cgttgctcat catcgcctcc ctggcccatg tactggtcgg
     8101 ctacggcgtg ttcctgttgt gcgcgcacgg ctggagagcg ccggtgagcg cgttggcctg
     8161 tgccatggcc accgtgttgt gcacccgatt gaccttctac tcgcccgtgc tggaggccaa
     8221 ctacctgctg tgcttgccct tcctgctcct gggctacctc gaagcacgtg ctcgcgccct
     8281 cgcccccgcg cacgccg
//
LOCUS       DAOQOC010000004         7810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3488_length_7810_cov_3.776273, whole genome shotgun sequence.
ACCESSION   DAOQOC010000004 DAOQOC010000000
VERSION     DAOQOC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3488_length_7810_cov_3.776273"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1560
                     /locus_tag="PL106_00110"
     CDS             211..1560
                     /locus_tag="PL106_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNA31494.1"
                     /translation="MRKTVLLPFLAVATFGHAQVFTSGLETWQGDTLPTDWFGVKTNL
                     EYDSIAQVSANVHGGSFAVQLINRENNTRRFSTQPLSVDSAQSYDVSFWVRGSGEVRV
                     SMFDGRQENSGYAATGPYTTVNGDTWTQVTGSVVCDHTNPAGEFIIYVRNTLPPGDVV
                     VDDVNISAGVVEPPVEASIYQIQFTTDQGGASTYNGNVVITGGIVTGVDTIGADAYFL
                     QAGTGPWSGIYVYDPAHTVAIGDSVTLTAAVDEFSNATTELINVSGFAIVSSGNALPA
                     PQPLNTSNATEEQWEGVLAIIANAQCSGLPDQYNEWGITQPSGPYLVDDLMYAYTPTL
                     GTNYDITGCIHYANSAWKIEPRFAADVVLATGIGETIFNNATIGPNPATDLLRISTGL
                     PDGTRVDLLLTDATGRTVRTGRINGSTVLDVHELSSGPYHLTLRTDKGTKSFAVQVH"
     gene            complement(1744..3861)
                     /locus_tag="PL106_00115"
     CDS             complement(1744..3861)
                     /locus_tag="PL106_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA31495.1"
                     /translation="MNAQKFKQRMAETAAEVFDYPKMAAIYQDITASGKATPDDWRRL
                     ALAYRRMGDPLKAEASYKQMANMGPLGVEDRLAYADVLRDNGHYSEAVEQYKAVLVTD
                     AENVRAKAFLQQPDLFSRLIKGNTRNSVRTVPINSPQADLGLTVMEDLLIFSSARGEG
                     VGGKSDYNWDGQPFLNMYTALLKGQTAEAPLVLRKDINHRYHDGTASYDSLAKRLYFT
                     RDNVWYGTISKAANGELKLAIFYSDIKVGEFNQPEWGALAPFEHNDPEYNTGHPCISP
                     EGRRLYFVSDRPGGQGGTDIWYSDNLGGEWGAPKNMGPKVNTSGNEMYPYVTADSVLY
                     FSSNGHPGLGGYDLFYCRLGASGPGGVLNLGAPLNSRFDDRNLILMKDDSTGFFVSDR
                     EGGKGSDDIYGCTVHPPMMTIRGRVVDSKTREPLDGARIDLRNGAGRFMDEARIVMLD
                     GGRFEIEAPITDPYVITATKNGYLQNTVNISPGTDALDDVVLALDKYDYGAEGVVMHG
                     ETMAPIAGAKVGLYDAEDKLLSEEITETDGRYSFTLEAEKDYRIKVDKEGFFKQTARI
                     STKGRPSTIIKTDFKLFPLDVGTVVRLDNIYYDLAKWAIRPDAALELDKLVQTLNDNP
                     TVSIELSSHTDCRGKDAYNLSLSEKRAKSAVEYLIKQGIAKERLTSKGYGETKPVETC
                     DCTKCNEDQHQRNRRTEFKVLGK"
     gene            complement(3964..4956)
                     /locus_tag="PL106_00120"
     CDS             complement(3964..4956)
                     /locus_tag="PL106_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA31496.1"
                     /translation="MIRSTRQPMRTMTHRIARWTGLAAMALVLAPGATRAQQDPQFTQ
                     YMFNQLAINPAYAGSAERVSLKALSRHQWVGFEGAPSTQTLTVHSPLPFESVALGGTI
                     MRDQHGPVTQYGFMLDVAYRFFVSRNTKLAFGIKGGMNLFQGRFADLSPLQQNDQVFQ
                     QNTQQKLDPQFGFGIMWYGDRHYLGISTPKLLRTDLFDDGAYSADTLGGSVWQAGQRA
                     HWFLSGGYVFDMGLYHKFKPTFLVKAVNGAPISFDLSANFLLYDKLWLGAMYRHTDAV
                     GLLAQYNITHGFSIGYAYDYPITMLNKYSGGSHEFMLCFEFGNKLKGIRSPRYF"
     gene            complement(4969..>7810)
                     /locus_tag="PL106_00125"
     CDS             complement(4969..>7810)
                     /locus_tag="PL106_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA31497.1"
                     /translation="PATGTWTIISGTAVITDPSDPHSSVTGIGVGVLELEWTVSNGPC
                     GPSSDQVSIELFDLNSPPASAGPDQEFCTPNTSTVLAGNAPTPPGTGVWSVFQGAALF
                     ADDSDPGSGVSGLPVGENILVWTLEQGVCGTSHDSVSVFIFDAEASGASAGPDVEQCM
                     PMDSTFLAGNAPVFPAHGTWTVIEGAGMIDDPNDPGTLVTNLAIGNNTFVWQMDNGPC
                     PNGLTTDTVTVVLFSDSTDAPDAGPDLETCLPLTFAQLEGEAPTPPASGGWTVIGGVG
                     NVADPTDPQSMITDLEVGITTLVWTLDLGPCPNNGLLTDTVDIYIYDPSAPLADAGPD
                     QQLCTPEDTTLMQALIPTFPGEGTWTLVGGSGTISDIHDPAAMITGLSVGLNQFEWMV
                     YNGNCGQGPPSRDTVTIAVFDGNAPLADAGADQQLCAPETSTQLGANSAVFPGTGTWS
                     IITSGGGVSNASDPQSMASSFPVGDNLLVWTIDNGACGTSTDTLLIAVFDGSAQPANA
                     GPDQDICVPTQPNEVTMAANAPVYPASGQWTITGTGTIADPADPHTLVTGMQPGITVL
                     TWTIDNGPCGPVTTDQMQIITHDAGAPVAQAGPDMELCTPTGEITMVGNPPQFPATGA
                     WTVVNGSGTLVDPTSPNSGITGLEPGVSVLVWSIDNGACGTTTDTLTISVYDNTSAAS
                     NAGDDREFCDPDAVDVVMLGSVPAPPATGAWELVAGSGTITDPTNPFTAVTGIGFGVN
                     VFQWSVDNGVCGTSSDEMTITVYDQNEQPASAGEDRTQCHDVTSAALNAAPLVSQTAT
                     GLWTLIAGTGDIDTPTAPSTEVENLTIGQNILVWTVTNGVCPATTDTVIIGVNDCSKI
                     RIPDAFSPNGDGVNDTYMIEGLLYYRQNSIQVFNRWGTRVLERSPYNNDWDGRSEASL
                     NWGEELPESTYYYILDLGNGDEPYTGYIYLKR"
BASE COUNT         1415 a         2462 c         2365 g         1568 t
ORIGIN      
        1 actgagcccg ggcagctgga cgtggacacc ttcgggggca ctggaccttt cggcctacct
       61 ctcgaacaac acccgtatcg cgttcaagta caccggcagt gccagcgatg gcaagacctg
      121 ggaactggac gatatcaaga tcacggtaca ataggaagta cccgaccccg agaacaccca
      181 acgcccgcgc cgggctccgg cgtacaaagc atgagaaaga ccgtactcct tcccttcctc
      241 gcggtggcca ccttcggtca cgcgcaggtt ttcaccagtg gcctggaaac ctggcagggc
      301 gataccctgc ccaccgactg gttcggtgtg aaaaccaatc tcgaatatga cagcatcgca
      361 caggtgagcg ccaacgtgca tggtggctct ttcgcggtcc aactgatcaa ccgcgagaac
      421 aacacgcgcc gcttcagcac ccaaccgctg tcggtggaca gtgcgcaatc ctatgatgtc
      481 tccttctggg tgcgcggatc cggcgaagtg cgcgtttcca tgttcgatgg acggcaggag
      541 aacagcggtt atgccgcgac cggaccgtac accaccgtga acggcgatac ctggacccag
      601 gtgacgggca gcgtggtgtg cgaccacacc aacccggccg gcgaattcat catctacgta
      661 cggaacacgc tccccccggg cgatgtggtg gtggatgatg tgaacatcag tgcaggggtc
      721 gtggagcctc ccgtggaagc gagcatttac cagatccaat tcaccacgga ccagggcggt
      781 gcgtccacat acaatgggaa cgtcgtgatc acgggtggca tcgtcaccgg tgtggacacc
      841 atcggtgcgg acgcctattt ccttcaggcg ggtacgggcc cctggagcgg gatttacgtg
      901 tatgacccgg cgcataccgt ggcaatcggc gatagcgtga cgctcaccgc ggcggtggat
      961 gagttcagca atgccaccac cgaactgatc aacgtatcgg gcttcgccat cgtgagcagc
     1021 ggcaatgcgc tgccagcacc gcaacccttg aacacctcca atgccacgga ggagcaatgg
     1081 gaaggtgtat tggccatcat cgccaacgca caatgcagcg gtcttccgga ccagtacaat
     1141 gaatggggca tcacccagcc cagtggcccc tatctggtgg atgacctgat gtacgcgtac
     1201 acaccgacac tcggcacgaa ttacgacatc accggttgca tccattacgc caacagcgca
     1261 tggaagatcg agccgcgctt cgcagccgac gttgttctgg ccaccggtat cggcgagacc
     1321 atcttcaaca acgccaccat cggccccaac ccggccacgg atctgctgcg tatcagcacc
     1381 ggactgcccg acggcacccg cgttgatctg ctcctcaccg atgcgaccgg tcgcacggtg
     1441 cgtacgggtc gcatcaacgg cagcaccgta cttgatgtgc atgaactgag cagcggaccg
     1501 taccacctga cgctgcgcac ggacaagggc acgaagtcct tcgcggtaca ggtacactga
     1561 acggcgcttg cccgtccgat catgccgaag gccgatccgc aagggtcggc cttcgttgtt
     1621 cacacgctcc gttgtgccca aaaagcgcgc ggaccgatcg ctcggcccgc gtgctccctt
     1681 tcgtcaactg gacaactccc ggcttgcgcc caggagatcc cgacagcgtc gggacaccca
     1741 accctacttg cccagcacct tgaattccgt tcgcctgttg cgttggtgtt gatcctcgtt
     1801 gcacttggtg caatcgcagg tctccaccgg tttcgtttcc ccgtagccct tgctggtaag
     1861 tcgctccttg gcgataccct gtttgatcag atattccacc gcgctcttgg cccgcttctc
     1921 cgacaggctg aggttgtacg cgtccttccc gcggcaatcg gtgtgtgaac tgagctcgat
     1981 ggacaccgtg ggattgtcgt tcagcgtctg caccagtttg tccagttcga gcgcggcatc
     2041 gggccggatc gcccacttgg ccaggtcata gtagatattg tccagccgca ccacggtacc
     2101 cacgtccagc gggaagagct tgaagtcggt cttgatgatg gtgctgggcc gacccttggt
     2161 actgatgcgc gccgtctgct tgaaaaatcc ctccttgtcc accttgatcc ggtagtcctt
     2221 ctccgcttcg agcgtgaagg agtatcgacc atcggtctcc gtgatctcct cggacaggag
     2281 cttgtcctcc gcatcgtaca ggccgacctt ggcaccagcg atcggggcca tggtctcgcc
     2341 gtgcatcacg accccttcag caccataatc atacttgtcc aacgcgagca ccacatcgtc
     2401 caacgcatcg gttccggggc tgatgttcac cgtgttctgc aagtaaccgt tcttggtggc
     2461 ggtgatcacg tacggatcgg tgatgggtgc ctcgatctcg aagcggccgc catcgagcat
     2521 cacgattcgt gcttcgtcca tgaatcggcc ggcaccgttg cgcaggtcga tcctggcgcc
     2581 atcgaggggt tcacgggtct tgctgtccac cacgcggccc cggatggtca tcatcggcgg
     2641 atgcaccgtg caaccgtaga tgtcgtcgct gccctttccg ccctcccggt cactcacgaa
     2701 gaagccggtg ctgtcatcct tcatgaggat gaggttgcga tcgtcgaaac gcgaattgag
     2761 cggtgcgccg aggttgagga cgccgcccgg tccggacgca ccaagtcggc agtagaacag
     2821 atcgtagccg cccaggccgg ggtgaccgtt gctgctgaaa tagagcacgc tgtcggccgt
     2881 gacgtacggg tacatctcat tcccggacgt gttcaccttc ggtcccatgt tcttcggtgc
     2941 accccactcc ccgcccaggt tgtcgctgta ccagatgtcc gttccaccct gtccgcccgg
     3001 tcggtcgctc acgaaataga ggcgcctccc ctcggggctg atgcacggat gaccggtatt
     3061 atactccgga tcgttgtgct cgaaaggggc gagtgctccc cactcgggct gattgaactc
     3121 cccgaccttg atgtcgctgt agaagatggc cagtttgagc tctccgttcg cggctttgct
     3181 gatcgtgccg taccacacgt tgtcgcgggt gaagtacagg cgcttggcca gcgagtcgta
     3241 tgaggcggta ccatcgtggt accggtggtt gatgtccttg cgcagcacca acggcgcttc
     3301 cgcggtttgc cccttgagca acgctgtgta catgttcagg aagggctgcc catcccagtt
     3361 gtaatcggac tttccaccaa ctccttcccc gcgcgcgctg ctgaaaatga gcaggtcctc
     3421 catgaccgtg agccccaggt cggcctgcgg gctgttgata ggcaccgtgc gcacgctgtt
     3481 gcgtgtgttg cccttgatca gccgactgaa aagatcgggt tgctgcagga acgccttcgc
     3541 ccggacattc tccgcatcgg tcacgagtac cgccttgtat tgctcgaccg cctcgctgta
     3601 atgcccgttg tcgcgcagca catcggcata ggccagccgg tcctccaccc cgaggggccc
     3661 catgttggcc atctgcttgt aactggcttc cgccttcagc gggtcaccca tgcgccggta
     3721 cgccagcgcc aacctgcgcc agtcatccgg ggtcgccttc ccactggccg tgatatcctg
     3781 gtagatggcg gccattttcg gatagtcgaa cacctccgcc gctgtttcgg ccatgcgctg
     3841 tttgaacttc tgtgcgttca tggacgccgg taggataagc agggccagga caacggtcca
     3901 cggaatggac ctcccgccga aggctttgcc agtcgctgcg cagtatcgcg tggggatcat
     3961 tggtcagaag tagcgcggtg aacggatgcc cttcagcttg ttgccgaact cgaagcagag
     4021 catgaactcg tgcgagcctc cggaatattt gttgagcatg gtgatcgggt aatcatacgc
     4081 gtacccgatg ctgaagccat gggtgatgtt gtactgcgcc agaaggccca ccgcatcggt
     4141 gtggcggtac atcgccccca accacagctt gtcgtacaag aggaaattgg cgctgaggtc
     4201 gaagctgatg ggcgcaccgt tcacggcctt caccaggaag gtgggcttga acttgtggta
     4261 cagtcccata tcgaagacgt agcctccact gaggaaccag tgcgcacgct gaccggcctg
     4321 ccacacactg cccccgagcg tgtccgcact gtacgcccca tcatcgaaga ggtccgtacg
     4381 caacagcttg ggtgtactta ttcccaggta gtgccggtcg ccgtaccaca tgatgccgaa
     4441 cccgaactgc gggtccagct tctgctgcgt gttctgctgg aacacctggt cgttctgctg
     4501 caaggggctc aggtcggcga agcgcccctg gaagaggttc ataccgccct tgatcccgaa
     4561 tgccagtttc gtgttccggg aaacgaagaa gcggtaggcc acatcgagca tgaacccgta
     4621 ttgcgtgacg gggccgtgct ggtcgcgcat gatcgtgcct cccagtgcca cgctctcgaa
     4681 cggcagcgga ctgtgtaccg tgagcgtctg cgtgctgggg gcgccctcga aaccgaccca
     4741 ttgatggcgg ctcagggcct tcaggctcac ccgctcggcg ctgcccgcat acgcgggatt
     4801 gatcgccagc tggttgaaca tgtactgggt gaactgcggg tcctgctggg cccgggtcgc
     4861 accgggtgcg agcacaagag ccatggccgc aagccctgtc catctggcga tacggtgcgt
     4921 catcgttcgc atcggttgcc gggtcgatcg gatcatcgtt ccggtgtgtt agcgtttcaa
     4981 gtagatgtat cccgtgtagg gctcgtcacc gttgcccagg tccaggatgt agtagtacgt
     5041 gctctcgggg agttcctcgc cccagttcaa gctcgcctca ctacggccat cccagtcgtt
     5101 gttgtacgga ctgcgttcaa gcacacgcgt tccccagcgg ttgaagacct ggatactgtt
     5161 ctggcggtaa tagagcaggc cctcgatcat gtacgtgtcg ttcaccccgt ccccgttcgg
     5221 gctgaacgcg tcggggatac ggatctttga acagtcgttc accccgatga tcaccgtatc
     5281 ggtcgtcgcc gggcacacgc cgttggtcac ggtccacacg aggatgttct gcccgatggt
     5341 caagttctcg acttccgtgg agggcgcggt cggcgtatcg atgtcgccag tacccgcgat
     5401 caaggtccac aggcccgttg cggtctggga aacgagcggt gcggcgttca gcgcggccga
     5461 ggtcacgtcg tggcactgcg tccggtcctc acccgcactc gccggctgct cattctggtc
     5521 gtacacggtg atggtcatct catccgagct ggtaccacag accccgttgt ccacggacca
     5581 ttggaacacg ttcactccga agccgatacc tgtcactgcc gtgaaaggat tcgtcggatc
     5641 ggtgatggtg cccgatccgg cgaccagttc ccatgcaccc gtggccgggg gggcgggaac
     5701 ggaacccagc atgaccacat caacggcatc cggatcacag aactcccgat catccccggc
     5761 gttcgaggcg gcggatgtgt tatcatacac cgaaatggtg agcgtatcgg tggtggtacc
     5821 gcacgctccg ttgtcgatcg accatacgag cacggatacg cccggctcga ggccggtgat
     5881 cccgctgttg ggcgatgtgg gatcgaccag ggtgcccgat ccgttcacca cggtccaagc
     5941 gcccgtggca gggaattgtg gcgggttgcc caccatggtg atctcgccgg ttggggtgca
     6001 cagctccatg tcgggtccgg cctgggcaac cggcgcgccc gcgtcgtgcg tgatgatctg
     6061 catctgatcg gtcgtgaccg gaccgcacgg gccgttgtcg atcgtccagg tgagcactgt
     6121 gatgcccggc tgcatgcctg ttaccaaagt atggggatcg gcagggtccg cgatcgtgcc
     6181 ggtgccggtg atggtccact gtccgctggc cggataaacc ggggcattgg cggccatggt
     6241 cacctcattc ggttgtgtgg gtacgcagat gtcctgatcg ggacccgcat tggccggttg
     6301 tgccgagcca tcgaataccg cgatgagcaa ggtgtccgtg ctcgtaccgc acgcgccgtt
     6361 gtcgatcgtc cagacgagca ggttgtcgcc cactgggaag gaactggcca tgctctgcgg
     6421 gtccgaagcg ttggacacac cgccacccga ggtgatgatg gaccaggtgc ctgttcccgg
     6481 gaagaccgcg ctgttggctc caagttgcgt actggtctcc ggagcgcaca actgctggtc
     6541 ggcacccgca tcggccaacg gagcattccc gtcgaatacg gcaatggtca ccgtatcacg
     6601 cgagggcggt ccttggccgc agttcccgtt gtacaccatc cattcgaact ggttgagacc
     6661 gacactcagc cccgtgatca tcgccgcggg atcatggatg tcagaaatgg tcccgctacc
     6721 acccacgagg gtccacgtgc cctcacccgg gaaagtcggg atgagcgcct gcatgagtgt
     6781 ggtgtcttcc ggcgtgcaga gctgctggtc aggacccgca tcagccagtg gggccgatgg
     6841 gtcatagatg taaatgtcca ccgtgtccgt cagcaggccg ttgttcgggc agggtcccag
     6901 atcgagcgtc catacgagcg tggtgatccc cacttcgaga tcggtgatca tcgattgcgg
     6961 gtccgtggga tcggccacgt tgcccacgcc cccgatcacc gtccaaccac cgctggcagg
     7021 gggtgtggga gcctcacctt ccagttgtgc gaacgtgagc ggcagacagg tctccagatc
     7081 agggcccgca tcaggtgcgt ccgtactgtc gctgaagagg accaccgtca cggtatccgt
     7141 cgtgagcccg ttcgggcacg gaccgttgtc catctgccag acgaaggtgt tgttcccgat
     7201 cgcgaggttg gtgaccagtg taccgggatc attcgggtcg tcgatcatgc ccgccccctc
     7261 gatcaccgtc caggtgccat gcgccgggaa caccggggcg ttgcccgcca ggaacgtgct
     7321 gtccatcggc atgcactgct ccacatccgg tcccgcggac gcgccggaag cctccgcatc
     7381 gaaaatgaat acgctcacac tgtcgtggct ggtaccgcac acaccctgct ccaaggtcca
     7441 tacgaggatg ttctcgccga caggcagccc gctaacgccc gatcccgggt ccgagtcatc
     7501 cgcgaaaaga gccgcgccct ggaagacgct ccaaacaccc gtacccggag gagtgggtgc
     7561 gttgccggca agaacggtac tcgtattcgg tgtacagaac tcctggtcgg gaccggcgga
     7621 ggctggtggc gagttcaggt cgaacagctc gatgctcacc tggtcgctgc ttggaccgca
     7681 cggtccgttg ctcacggtcc attcgagttc aagcactccc acaccgatcc cggtgaccga
     7741 cgaatgcggg tcgctcgggt cggtgatcac agccgtgccg ctgatgatcg tccaggtgcc
     7801 cgtggccggg
//
LOCUS       DAOQOC010000005         7780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3508_length_7780_cov_3.615922, whole genome shotgun sequence.
ACCESSION   DAOQOC010000005 DAOQOC010000000
VERSION     DAOQOC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3508_length_7780_cov_3.615922"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PL106_00130"
     CDS             complement(<1..378)
                     /locus_tag="PL106_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNA31498.1"
                     /translation="MHALRTPLLLFFQLAVLCSWAQGTVDGRVRDKKDQSSLPGASVL
                     LLLADDTTQRRSAVADPDGLFRIDNVAEGQYILRAAYTGYVTAQRNIDVPGSTTGLMF
                     DLESSVVTLKEVEASAVMKRVEQK"
     gene            528..833
                     /locus_tag="PL106_00135"
     CDS             528..833
                     /locus_tag="PL106_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNA31499.1"
                     /translation="MKEKFLRFIPARLRNRYGLGLGLFVLYVCFLSDYDLYTTLKLRA
                     ELSKKRSEHERYEQDIRTVRAELHELNGDMALLEKFAREKYKMKRDDEDVFVLVARR"
     gene            864..1634
                     /locus_tag="PL106_00140"
     CDS             864..1634
                     /locus_tag="PL106_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNA31500.1"
                     /translation="MRLSYSCLILVALLVPDAVHAWGPTGHRTVGQVAEQHLSRKAKR
                     AVYHILGTQSIAMASTWMDDVRSDHAWDHVRDWHWVTIPDGSTYAAVEKNPKGDVVEA
                     IARMQALLKSDTVPLERQRIHLRMLIHLVGDLHQPLHVGRGDDKGGNDFQVQWMKKGS
                     NLHRVWDSGMIELEELSYSELAASLDRPSKERVRAWQLGSVANWAEENIVLRPAIYAV
                     EPGDNLGYEYKYINWPVVREQLLKGGIRLAALLNAIYG"
     gene            complement(1702..2130)
                     /locus_tag="PL106_00145"
     CDS             complement(1702..2130)
                     /locus_tag="PL106_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA31501.1"
                     /translation="MRNNEGETLDSSSGRDAFAYIQGAHQIVPGLEQQMEGRKAGEKF
                     TAVVPPDMGYGDFDKNLLQRVPLERFNGQKVETGMQFQAGNQGIVTVREVADGQVLLD
                     GNHPLAGVTLNFEVEITAVRTATPDEVSHGHVHGPGGHHH"
     gene            complement(2036..2899)
                     /locus_tag="PL106_00150"
     CDS             complement(2036..2899)
                     /locus_tag="PL106_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNA31502.1"
                     /translation="MRFTFYGPDFLLQLLLDHNAGDTISRPASIQITDNSGTYVPDSV
                     GVRLKGNSSFDFYPTDKKPLKLDFNEFISGHVYHGLKKLNLANCWSDPTFLREKVFFE
                     ICRSEGVLAPRMSFANVYLNDVHWGLYGAIEQVDKTLLDRWLGDNAGNLFKAGDNYTP
                     DGGGLGLEADLKHYGPAAEDYAGRNELKTNEARNDWSDLIGYRQEVRSVRYGRSPSPL
                     RGGEAVGVEVHTFATRPRQAWAITHDHFQQLRSQFPLHTAQQRGRNTGFLLWQGRLRV
                     HSGGASDRAGS"
     gene            complement(3131..6847)
                     /locus_tag="PL106_00155"
     CDS             complement(3131..6847)
                     /locus_tag="PL106_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNA31503.1"
                     /translation="MKGLFTLCAFALAPAALMAQFSPVRFVEETEFALLEFGLLHDYD
                     GDGDIDMAMCSTEGAGWWRNDGTGGFSPVEWVHRVPFGMYAELLAAGDMDGDGDIDLL
                     SAVNYTVMYSYLNDGTGNFSFVDSIQVPDYDMRDMLLEDLDGDGKADILFGDYFSGMV
                     AYRSSLGDGTFGPEQVIGSLPGAMDLHVADIEGDGDNDVLISGYGSMGCHICVNQGGG
                     VFLDAALLIPSTTDIMDVKTADMNGDGDLDVITAAYTTSRWYPNDGAGQFIVENTIIQ
                     EPGLDLWSNGLGDIDGDGDMDAVPATYDGQVPVWYANDGAGNFAAVGALTQATTFRPA
                     FADIDGDGDEDLVQVQRNALWWPNDGTGVMATSKIIAGTLRNLATVGLADMDGDGDQD
                     ALAAGVLDDRIVMYANDGTGQFGGQITLLDTMSGMGEVVPGDLDGDGDMDMVVCSGSL
                     SYACFNDGTGSISSVQIVDNDPGTIKGTSVGDVDGDGDLDLIHAEYNPNRIRLWRNTG
                     SGSFDPPVIVFSGFNPTSCDLLDADGDGDLDITSYYGQNTHYFIWIANDGSGSYGAAQ
                     VLGSMSGGGGQCSAIALDADADGVRDLVYVSSEGLNMYRGLGGGTFSAAVNIDPNYMD
                     ESDAFPIDLDSDGDMDIALAEKSGAGARYYLNDGTGVFSSATWFDATWLGDMASELSI
                     GDLDGDGDPDALVGNMNGQGFYAVGWVENYFNSPYHIDGTLFHDVNENGAQDVGEGPL
                     PFVAVHADPVASVPLTDPDGHYSILTDPGDYEVSVPFPNAWWGLTTSPATYNVQLTTG
                     TPVSTGNDFGYAPIVDTTVVELGIAANIGVCGDTTLVYLNVMNVGTSQPSGTFCGMID
                     TLFTIISFDPPPTSVTGNMYCWEIDSLYYYTPFTIVAEVVVPDASYMGTLLVGGTQLT
                     ETDGQGNVLQTWQEAWAIPLTCSYDPNYKEVEPKGYGEAGAVDIDTERLEYTIHFQNT
                     GTASAQDVVLVDRLAEEIDPSSLQVLAYSHAPTAINLETDRDMVIRFDGIQLPDSGTT
                     FTGSQGFIRFAVDLVPGLPHATVIENTSNIHFDLNVPVITNTTVTTLVDCALAEAAIS
                     LSGSIALEVTEGDAYQWYLNAEPIAAATGQVFFPNENGSYTCEVTTTYGCIVMTDPYQ
                     VTSVSVPLIENLRLALLPNPMNATARLVFSEALRPGDRVQLLDTRGAEVMSVPGNGAS
                     SIVIDRDGLVNGLYMVRVLRAGATLATLRLAVVD"
     gene            7110..>7780
                     /locus_tag="PL106_00160"
     CDS             7110..>7780
                     /locus_tag="PL106_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31504.1"
                     /translation="MADETLFEAFPPATRAQWEAVIKKELKDKELSTLHVLVNGTALP
                     PFHMAGESGPIGTRRRGVERDGNAWRGTFEVEASAANANDRVLEALMNGMDSVRVTGD
                     LARLPEVLKDVLFGAIDLQLNGGDVATLRWLEHEAERQGIMPAELCLCLGMPHDAAVA
                     DLQEPLQAFPLVRLFEVDDPADKEALRIGRDLLVRLLAMGFSTDNACARIQFRLHLGD
                     DLFVEA"
BASE COUNT         1508 a         2315 c         2440 g         1517 t
ORIGIN      
        1 tttctgctcc acgcgcttca tcacggcgga ggcttccact tccttcaggg tgaccaccga
       61 actctccaga tcgaacatga gcccggtggt gcttcccggt acgtcaatgt tgcgctgcgc
      121 cgttacatac ccggtgtacg cagcgcgcag gatgtactgg ccctcggcca cgttgtcgat
      181 acggaacaga ccgtccggat cagcaacagc gctacggcgt tgagtggtgt catccgccag
      241 caggaggagt acgctggcgc cgggcaggga actctggtcc ttcttatcgc gtacccggcc
      301 atccacggtg ccttgagccc acgaacacag gaccgccaac tggaagaaga gcaggagcgg
      361 ggtccgaaga gcgtgcatac gggatcgggc gtgccggacc gcgttggtcg cggccggcat
      421 ccgccaaatg tgggcattgc tcgggcccat ccgtttgaac gatcgatcgt tcatcaacga
      481 tccaggagcg atgaagcggc ggcgcggcct ctgggcatct tcgtgccatg aaggagaagt
      541 tcctgcggtt cattccggca agactgcgga accgatacgg cctgggcctc gggctgttcg
      601 tgctgtacgt ctgtttcttg agcgattatg atctgtacac cacgctgaaa ctccgtgcgg
      661 agctcagcaa gaagcgcagc gagcatgagc gttacgaaca ggatatccgc acggtccgtg
      721 cggaattgca tgagttgaac ggggatatgg ccttgctgga aaaattcgcc cgggagaagt
      781 acaagatgaa acgcgacgac gaggacgtgt tcgttctggt ggcgaggcgg tagagccgca
      841 tctttgcgcg catgggaccg aacatgcgcc tttcgtacag ttgcctgatc cttgtcgcgc
      901 ttcttgttcc tgatgccgtg catgcgtggg gccccaccgg gcaccgcacg gtgggccaag
      961 tggccgagca gcacctgagc aggaaggcga agcgcgcggt gtaccatatc cttggtacac
     1021 aaagcatcgc catggccagc acctggatgg atgatgtccg gagcgaccac gcatgggacc
     1081 atgtgcgcga ctggcattgg gtgacgatcc ccgatgggtc cacctatgcc gcagtggaga
     1141 agaacccgaa gggcgatgtg gtggaggcca tcgcccgcat gcaggcattg ctcaagtcgg
     1201 acacggtccc gttggaacgg cagcgcatcc acctgcgcat gctcatccac ctggtgggcg
     1261 acctgcatca gccgttgcat gtggggcgcg gcgatgacaa gggtggcaac gacttccagg
     1321 tgcaatggat gaagaagggc agcaacctgc atcgcgtatg ggacagcggg atgatcgagc
     1381 tggaggaatt gagttactcg gaactggccg cttcactgga ccggccatcc aaggagcggg
     1441 tgagggcatg gcaactggga agcgtggcga actgggccga agagaacatc gtactgcgac
     1501 cggcgatcta tgcggttgag cccggcgata acctcggata cgagtataaa tacataaact
     1561 ggcccgtggt gcgcgaacaa ctgctcaagg ggggcatccg gttggcggcc ctgctcaacg
     1621 cgatctacgg atagggtcgg gaagtgctcc gtgagggcgt gggagccttg cccatcccca
     1681 ggacacagcg atgcggagaa ctcagtgatg atgaccgccc ggcccatgca cgtggccgtg
     1741 gctcacctcg tcgggcgtgg cggtgcgaac cgcggtgatc tccacctcga agttgagcgt
     1801 tacccccgcc agcggatggt tgccatcaag cagcacttgt ccgtcggcca cttcgcgtac
     1861 cgtgacgata ccctggttac cggcttggaa ttgcatgccg gtttctactt tctgtccatt
     1921 gaaccgctcc aggggcacgc gttgaaggag gttcttgtcg aaatcgccgt agcccatgtc
     1981 gggcggaacg acagcggtga acttttcacc ggccttccgg ccttccattt gttgttcaag
     2041 acccggcacg atctgatgcg ccccctgaat gtacgcgaag gcgtccctgc cagaggagga
     2101 atccagtgtt tctccctcgt tgttgcgcag tgtgtaatgg aaactgacta cggagttgtt
     2161 ggaaatggtc atgtgtgatc gcccaagctt gtcgggggcg ggtggcgaaa gtatgtacct
     2221 cgacgcctac cgcttcacca ccacggagcg ggcttgggct tcttccatat cgcaccgaac
     2281 ggacttcctg gcggtatccg atcaggtcgc tccagtcgtt ccgggcctcg ttggtcttga
     2341 gttcgttgcg acccgcatag tcctccgccg cgggtccata gtgcttcagg tccgcctcca
     2401 ggccgaggcc gccgccgtcg ggtgtgtagt tgtcaccggc cttgaagagg ttgcctgcgt
     2461 tgtccccgag ccatcgatcg agcaaggtct tgtccacctg ttcgatcgca ccgtacaaac
     2521 cccaatgaac gtcgttcagg tacacgttgg cgaagctcat gcgcggcgcg agcacacctt
     2581 cgctgcggca gatctcgaag aagacctttt cgcgcaggaa ggtgggatcg ctccagcagt
     2641 tggccaggtt gagcttcttc agcccgtgat agacatggcc gctgatgaac tcattgaagt
     2701 cgagcttcag cggtttcttg tccgtgggat agaagtcgaa ggagctgttg cccttgagcc
     2761 gcacgccgac gctatccggg acgtatgtgc ccgagttgtc cgtgatctgg atcgaggccg
     2821 gtcgcgagat cgtgtcccct gcgttgtggt ccaacaacaa ttgcaacaag aaatcggggc
     2881 cgtagaaagt gaaccgcatc tcgataacct ggtcggtatc gaagagcgca tcaccgggta
     2941 gctgggcacc tacggggatg agtgcggaca ggaatacggt ctacagggca tagcgaaggc
     3001 tcatgtggtg gtgcttgcct gatgacgcag gccgttccgg gcaccgttgg tccggaccag
     3061 cgacggaacc gccggagcgg gcagaccgcc ggatggaacg gtatgccgct cgcgggatcg
     3121 gtcctgggat ctagtccacg acggccaagc gcagggtcgc cagcgtggca ccggcccgaa
     3181 gcacacgcac catgtacagg ccattgacca gcccatcccg atcgatcacg atcgacgaag
     3241 cgccattgcc gggcacgctc atcacttccg cgccgcgcgt atccagcagt tgaacgcgat
     3301 cacccggccg cagcgcttcg ctgaagacca gtcgggccgt ggcgttcatc gggttcggaa
     3361 gcagggcgag ccgaaggttc tcgatcagag gtaccgagac gctggtcacc tgatacggat
     3421 ccgtcatcac gatacatcca taagtggtcg tcacctcaca ggtgtaggaa ccgttctcgt
     3481 tcgggaagaa cacctggccc gttgcagcag cgatgggttc agcgttcagg taccattggt
     3541 aggcatcgcc ttcggtgacc tccaatgcga tggaccccga aaggctgatg gcggcctcgg
     3601 cgagcgcgca atccaccagt gtggtcaccg tggtgttggt gatcacggga acattcaggt
     3661 cgaagtggat gttcgaggtg ttctcgatca ccgtggcgtg cggtagaccg gggaccagat
     3721 ccaccgcgaa gcggatgaag ccctggctgc cggtgaaggt tgtgccgcta tccggcaatt
     3781 ggatgccatc gaagcggatg accatatcgc gatccgtttc caggttgatg gccgtgggcg
     3841 catgggagta tgccagcact tgcaatgaac tgggatcgat ctcctcagcg agccggtcga
     3901 cgagcacgac atcctgcgcg gacgcggtgc ccgtgttctg gaagtggatg gtgtattcca
     3961 gccgttcggt atcgatatca acggccccgg cttcgccata gcctttgggc tcgacctcct
     4021 tgtagttcgg atcatacgag cacgtgagcg gtatggccca ggcctcctgc caggtctgca
     4081 gcacgttgcc ctgcccatcg gtctccgtca gttgcgtacc gccgaccagc agtgtgccca
     4141 tgtagctcgc gtccggtacc accacttccg caacgatggt gaagggcgtg tagtaataga
     4201 ggctgtcgat ctcccagcag tacatgttcc cggtgacgct cgtgggcggt ggatcgaagc
     4261 tgatgatggt gaatagcgtg tcgatcatgc cgcagaaggt gccgctgggc tgtgaggtgc
     4321 ccacgttcat cacgttgaga tagaccaagg tggtgtctcc gcaaacgccg atgttggccg
     4381 cgatgccaag ctccactacc gtggtgtcga cgatgggtgc atagccgaag tcgttgccgg
     4441 tgctcacggg tgtgccggtg gtgagctgca cgttgtacgt ggctggcgag gtggtgagcc
     4501 cccaccatgc gttcgggaac gggacggaaa cctcgtagtc gcctggatcg gtgaggatgg
     4561 aatagtgtcc gtcgggatcg gtgagcggta cgctggccac ggggtccgca tgcacggcga
     4621 cgaaaggcag gggtccttcg cccacgtcct gtgcgccgtt ctcgttcacg tcatggaaga
     4681 gcgttccgtc gatatggtac gggctgttga agtaattctc cacccagccc acggcataga
     4741 acccttggcc gttcatgttg cccaccagcg catcggggtc cccgtcgcca tccagatcgc
     4801 cgatggagag ctcagaggcc atgtcgccca gccaggtcgc atcgaaccaa gtggcgctcg
     4861 aaaagacccc ggtcccgtca ttcaggtagt agcgcgcgcc tgctccactt ttttcggcca
     4921 gcgcgatgtc catgtcaccg tcactgtcga ggtcgatcgg gaacgcgtct gattcatcca
     4981 tatagttcgg gtcgatgttc acggctgctg agaaggtccc accaccgagg cccctgtaca
     5041 tgttcaagcc ctcggaggag acgtatacga gatcccggac accgtccgca tccgcatcca
     5101 gcgcgatcgc cgagcactgg ccgccgccac cgctcatgga tccaagcacc tgggccgcgc
     5161 catagctgcc agagccatcg ttcgcgatcc agatgaagta gtgcgtgttc tgtccatagt
     5221 aagaggtgat atccaggtca ccgtcgccgt ccgcgtcgag caggtcgcac gaagtgggat
     5281 tgaaaccact gaacacgatg actggcggat cgaaggaccc actgcccgta ttgcgccaga
     5341 gccggatccg gttcgggttg tactcggcgt ggataagatc cagatcacca tcgccgtcca
     5401 cgtcgcccac gctggtgccc ttgatggtcc cggggtcgtt gtcgacgatc tgcacggagc
     5461 tgatcgaccc cgtgccatca ttgaagcagg cataggagag cgagccggag cacaccacca
     5521 tatccatgtc accatcacca tccaggtcac ccggcaccac ctcgcccatg ccgctcatgg
     5581 tatcgagcag ggtgatctgt ccgccgaact gccctgtacc atcgttcgca tacatgacga
     5641 tgcgatcatc gagcacaccg gccgccaatg cgtcctggtc cccgtcgccg tccatgtccg
     5701 ccaatccaac ggtggccaga ttcctgagcg tgcctgcgat gatcttggag gtggccatca
     5761 cgcccgttcc atcgttgggc caccagagcg cattgcgttg cacctgcacg aggtcctcat
     5821 cgccatcccc atcgatgtcc gcgaaggctg gtctgaatgt ggtggcttgg gtaagcgcac
     5881 ccaccgccgc gaagttcccg gccccatcgt tcgcatacca gaccggcacc tggccgtcgt
     5941 aggtggcggg caccgcatcc atatcaccat caccatcgat gtcacccagg ccattgctcc
     6001 acaggtccaa gcctggttcc tggatgatcg tgttctccac gatgaactgc ccggcaccat
     6061 cgttggggta ccagcgcgag gtggtgtaag cggcagtgat cacgtccagg tcgccatcgc
     6121 cgttcatgtc cgcggtcttc acatccatga tatcggtggt ggagggaatg agcagcgcgg
     6181 catcaaggaa aaccccgcct ccctggttca cgcagatgtg gcaccccatc gaaccatagc
     6241 cactgatcaa cacatcattg tcgccatcgc cttcgatatc agccacatga aggtccatgg
     6301 cacccgggag tgaaccgatc acctgctccg gcccgaaggt gccgtcccca aggctggatc
     6361 ggtaggccac cattccgctg aaataatccc caaagaggat gtcagccttg ccgtctccgt
     6421 ccaggtcttc gaggagcata tcgcgcatat catagtccgg cacctggatc gagtcgacga
     6481 aggagaagtt gccggtgccg tcgttgaggt aactgtacat caccgtgtag ttcacggcgg
     6541 acaagaggtc gatatcgcca tcgccgtcca tgtcccccgc tgccaggagc tccgcgtaca
     6601 tgccgaaggg gacgcggtgc acccactcca ccggcgaaaa gccgcccgtg ccgtcgttgc
     6661 gccaccaacc cgccccctcc gtggagcaca tggccatgtc gatatcgcca tcgccgtcgt
     6721 aatcatgcag cagaccgaac tcaagcaggg cgaactccgt ctcctccacg aagcgcacgg
     6781 gtgagaactg cgccatcaat gcggccgggg cgagtgcgaa tgcacagagc gtaaagagac
     6841 ccttcatcgt gtgcgggttt gaatggtggt gaacggacgg aagacgaagg aacacgtcaa
     6901 gcgttgctcc gcaagggatc aatgggtgac gggagccatt catctccagc catgacgaga
     6961 ggtccggtga ttatgctgct tgctttgcga aagaacggtc tgaaattacg atctggccct
     7021 ggttctgaac gccggtggaa tgcaccgagg accggccgca tgcgcgatct tcgccaatcg
     7081 cctgcgttaa gcgcttcggc gaccgatgaa tggcagacga gaccctgttc gaggccttcc
     7141 cccccgccac acgtgcgcaa tgggaggccg tgatcaagaa ggaactgaag gacaaggaac
     7201 tgtccacgct tcacgtgctg gtgaacggca cggcacttcc gccctttcac atggccgggg
     7261 aaagcgggcc gatcggtacc cgccgccggg gggtggaacg cgatggcaat gcctggcgcg
     7321 gcaccttcga ggtggaggct tctgccgcca acgcgaatga tcgggtactg gaggcactca
     7381 tgaacgggat ggacagcgtt cgggtaacgg gcgacctcgc caggttgcct gaggtgctga
     7441 aggatgtgct tttcggtgcc atcgatctgc agttgaacgg cggcgacgtg gccaccctgc
     7501 gctggctgga gcacgaggcg gagcggcagg ggatcatgcc tgctgagctg tgcttgtgct
     7561 tgggcatgcc gcacgatgcg gccgtggcgg acctgcagga acccttgcaa gctttcccgt
     7621 tggtccgtct gttcgaggtc gatgatcctg cggacaagga agcattacgg atcggtcgcg
     7681 acttgttggt gcggttgctg gcgatgggct tcagcacgga caacgcctgc gcccgcatcc
     7741 agttccgcct tcatctgggg gacgacttgt tcgtggaagc
//
LOCUS       DAOQOC010000006         7729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3548_length_7729_cov_3.944749, whole genome shotgun sequence.
ACCESSION   DAOQOC010000006 DAOQOC010000000
VERSION     DAOQOC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3548_length_7729_cov_3.944749"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PL106_00165"
     CDS             <1..227
                     /locus_tag="PL106_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNA31505.1"
                     /translation="AMMHGIITGHPFIDGNKHTGYQLGRLLLQEGGYDVEASEDERYA
                     MVIRAASGQMEVEDIKAWLTDHSMAYRKEG"
     gene            complement(284..676)
                     /locus_tag="PL106_00170"
     CDS             complement(284..676)
                     /locus_tag="PL106_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA31506.1"
                     /translation="MNKKEIGMRLQQLRNDRHLQQKEVATVLGISPSAYCDRENGHVM
                     LSAVEMDKLAEFYGMSLDEFLRSGQPVLHMHDHSSQGFNAYHMQHQHGVSDDLMKQFI
                     DALTANARAIEKLAEQQVRMNDLLAQRG"
     gene            1169..1600
                     /locus_tag="PL106_00175"
     CDS             1169..1600
                     /locus_tag="PL106_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31507.1"
                     /translation="MTHGNHMKLERVTYERLNSRQKERFNFQKVSALLADYGFATLKL
                     DDDWQGADFIAVHITGEHLKVQLKSRLTFDTKYKCKGIHIVFPNDGNWYLIDHDKMLD
                     EFLKRFADTFAMTASWGKGGYSWNHLSREKLDLLKPFRLQP"
     gene            1910..2467
                     /locus_tag="PL106_00180"
     CDS             1910..2467
                     /locus_tag="PL106_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNA31508.1"
                     /translation="MQILFYLFAAISIISALFVVGARSPINSVIALIVCFLSIAGHYI
                     LLNAQFLAAVHIIVYTGAIMVLFLFVIMLLNLNRTVEPRKRTVTRIAAVASGGLFLLT
                     LLGAIKNGVQITPAAEFNAGTGLVKSVGQSLFGPFLVPFELSSVLFLSAIIGAVTLGR
                     KETGVKPMVKEGDEYAGLNQPERVA"
     gene            2607..2936
                     /gene="nuoK"
                     /locus_tag="PL106_00185"
     CDS             2607..2936
                     /gene="nuoK"
                     /locus_tag="PL106_00185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNA31509.1"
                     /translation="MNDVITAIRVVPLEHYVVLSMILFSIGLAGVLFRRNTIILLMCI
                     ELMFNSVNLLLTAFSAYHSDPGGQVFVFFIMLVAAAEVTVGLAILVMMKRNVDSVDIN
                     QLSRLKG"
     gene            2933..4861
                     /gene="nuoL"
                     /locus_tag="PL106_00190"
     CDS             2933..4861
                     /gene="nuoL"
                     /locus_tag="PL106_00190"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNA31510.1"
                     /translation="MTRDILATLVPALPLAGAILVAALRRKLKGNSAGILATLMVAGS
                     FVCSAMLFIGQEFVSVMHAANMHQQDAQEGGTHIVKLFDWINVSTMKVPFAFQIDALS
                     ITMMLIVTGVGSLIHLYSIGYMHDDERVASFFSMLNLFTFAMLLLVMGANFVVTYIGW
                     EGVGLCSYLLIGFWYRTPAYNYAARKAFVMNRIGDLGFVIGLMWLFSTLGTLNYTEVM
                     ARAGSLSTEAVTGITLLLFVGAIGKSAQLPLFTWLPDAMAGPTPVSALIHAATMVTAG
                     IFLLVRSSALFMFAPFTQDVVLWTGLITSLLTASIGVFQNDIKKVLAYSTVSQLGLMF
                     VALGVGAYSAAMFHVTTHAFFKALLFLGAGSVIHALGGEQDIRKMGGLKGVTKVTYVT
                     FLIGTLAIAGIPGLSGFFSKDAILAAAYEAHPAIWAVSLLISLLTCFYMFRLLFLTFF
                     GSYRGKAHPHESPLVMTLPLMVLAVLSVFGGLLNIPHLFGGHESMKHFLATAAEGISG
                     EHLTLSHTTEWTLMGVTTALIALTIVLAYNRFVKRTTLDPEPEKLPLLQRLIAHKWLV
                     DELYAKLFERPYGWLSAHFYSIAERQFMVPLMVGTGGVTNKVGKALRRLQSGNMSFYL
                     FGMVIGLIALLAWTLYKV"
     gene            4863..6356
                     /locus_tag="PL106_00195"
     CDS             4863..6356
                     /locus_tag="PL106_00195"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNA31511.1"
                     /translation="MILLALIAVPFLAGLLLLVARPNAQARSIAMVASLTCTVIVLAQ
                     CWNFGGAELTFIDRDWVPDWGLRLKMGYDGIGLLMLMLTAVIFPFIIGAGAKEEQDRP
                     ALANALLLFTQSFLFGVFTAQNALLFYIHYELSLIPLFLYLLYWGTPSLEGSSGRGGA
                     DRRTITMRFFIYTLFGGLALLFGIAYCVVQEPSHSADFSALHALRLPIDVQVWLFWAL
                     FLAFAVKLPIFPFHSWQPDTYTMAPMQGTMVLGGVLLKMGLFGVLRFVFPIVPQGTDH
                     WRDLVVWLSIIGAIYAGWIAFRQTDFKRLVAYSSLSHVGIMTAALFTWTTYGITGSLY
                     QAFAHAVLVVCMLYIVGAVERRMGTRDLSVMAGLKLKSPRLAALFFLVMVNGIALPLT
                     QSFVGEWLMFNGLWQMEGGAWMTAVAVCTIILGAVYMLYAYQRVMLGPETRVAPGTSL
                     NEVAEADANDHLFLVPLIAITLVFGSYPAPVLDLVQSSVQTLLSTLN"
     gene            6358..>7729
                     /locus_tag="PL106_00200"
     CDS             6358..>7729
                     /locus_tag="PL106_00200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNA31512.1"
                     /translation="MSSLILLSVLGVLLLYVGLFGAGDKAGASRVLAPIAVIGVCGAI
                     ALLVRDYQVNIPDFAHMLQWDDTGRLFSIGILGITALIFLFGMDYYAKEERDVAEQFA
                     LIAFSLVGALMLTSYTNLLALFLGIEVLSIPLYILAGGKRHSFRSSEASFKYFLMGSF
                     ASAFLLMGIALIYGVCASFELWRITEVAATSDKNILFILGGFFVLVGLTFKVAAAPFH
                     FWSPDVYEGAPTLVTAYMATVVKMAAFAGLLRFVSMSGMPMAVGYALVVMAAATLLVG
                     NIVALRQSHFKRLMAYSSIAHSGFLLMAIVSGTPDRDAALFYYTFTYSIATLGLFMVM
                     TIAKRAANGDEHIRIFRGLFRANPWMGLVVLLLLLSLAGIPLTAGFMAKFKVFQIALA
                     MGHVKLTVLAVVMALVGIYYYAVVIREAFTPAEETPALVVSPLNLLVISLCGIAVVVL
                     GVMPGWV"
BASE COUNT         1370 a         2349 c         2348 g         1662 t
ORIGIN      
        1 cagcaatgat gcatggcatc atcacgggac acccgttcat tgatgggaac aagcacaccg
       61 gatatcagtt gggccgtttg ctgctgcagg aaggcggata cgatgtggag gcctcggaag
      121 atgagcgcta tgccatggtg atccgggcgg cttcgggcca aatggaggtc gaggacatca
      181 aggcgtggct aacggaccat tccatggcat atcgaaagga aggctgagcc cccctctcct
      241 ctggcagatc ccggccacgc cccctgcgtt cgctgctgaa acactacccc ctttgcgcga
      301 gcaggtcgtt catgcgcacc tgctgttccg ccagcttctc gatggcccgc gcgttcgccg
      361 tgagcgcgtc gatgaactgt ttcatcaggt catcgcttac gccatgctgg tgttgcatgt
      421 gataggcatt gaagccttgc gatgagtggt cgtgcatatg caataccggc tggcctgaac
      481 gcaggaactc atcgaggctc atgccataga actcggccag tttgtccatc tccacggccg
      541 agagcatcac atggccgttc tctcgatcgc aatatgcgga ggggctgatg ccgaggaccg
      601 tcgccacttc cttctgttga aggtgccggt cattgcgcaa ctgctgcaag cgcatcccga
      661 tctctttctt gttcatgtgc agtggtcatc ggagcgccaa ggtatcggga aatccggatc
      721 cgattcggga aatgccggat ccgtgcggag cgaggggtgc tgggcgggcg tggactgtta
      781 tgcttgcgga cccatcaaat gggacgaaca ccggggaagc cgaaaaccgg acagaatagg
      841 cgatctcgac aacaacacga caatggctac gtactggtac caatgcagac actgtggaac
      901 aacgatccgc aaggacagct cacccaacag caacgggtgt ccggacagca gttcccacgc
      961 atggtccaac ctcgggacgg tgggtgactc caactaccaa tgcaagaaat gcggtacgac
     1021 cgttcaaacc agatccaccc caaacgccag tggatgcccg gaagccagct cccattcatg
     1081 gtccaagctc tgaccgttcg gtccatcacc ggagcggtca gggttcatga gcgctgtcgg
     1141 ttcttggcat cttgagggca gtaagaactt gacgcacggc aaccacatga agctcgaacg
     1201 ggtaacctat gagcggttga acagccgtca aaaggaacgg ttcaattttc aaaaggtatc
     1261 cgcgctgctc gcggactatg gattcgccac gctgaaactc gacgatgact ggcagggcgc
     1321 cgatttcatc gcggtgcata tcaccggtga gcacctgaag gtccaactga aaagccggtt
     1381 gacgttcgac acgaaataca aatgcaaggg catccacatc gtgtttccaa acgatggcaa
     1441 ctggtacctc atagatcatg acaagatgct cgatgaattt ctgaagcggt tcgccgacac
     1501 attcgccatg accgcctcat ggggaaaggg gggttactcc tggaatcatc tcagcaggga
     1561 aaaactggat ctgctgaaac ccttcaggct acaaccatag gcaatagcca tccaatgcga
     1621 ggggcttgcg gacgctggaa ataacaccgt caccccctgc atcccctttc ttttctggtg
     1681 ccctgtgatc gccccagtgg ctgtttgccc ttctctccca aatcctcccg ctcttcggct
     1741 tccgaagcgc atcgcgaagg ctcgcccggc ctcgtgcaac gccatgcccg ccgcgtaccg
     1801 gaggttcgtg ttggtgggac ctccgcgagg ggatgccgcc cgatctttct tcgttccggc
     1861 cctcctgcta cattcgccac cgttccgaac agggcccacg gcgtaccgga tgcagatcct
     1921 cttctacctc ttcgcggcca tctccatcat cagcgccctc tttgtggtgg gcgcccgcag
     1981 cccgatcaac agtgtgatcg cgctgatcgt ctgtttcctc agcatcgccg ggcattacat
     2041 cctgctgaac gcgcagttcc tggcggcggt gcacatcatc gtgtacacgg gggccatcat
     2101 ggtgctcttc ctcttcgtga tcatgctcct gaacctgaac cggacggtgg agccgcgcaa
     2161 gcgcacggtc acccggatcg cggcggtcgc ttcgggcggg ttattcctgc tcacgctgct
     2221 gggtgcgatc aagaatggtg tgcagatcac cccggcggct gagttcaatg cgggaaccgg
     2281 actggtcaag agcgttggac aaagcctctt cgggcccttc ctggtacctt tcgaactcag
     2341 cagcgtgctc ttcctgagcg cgatcatcgg tgccgtgacc ttgggccgca aggagaccgg
     2401 tgtgaaaccg atggtgaagg agggcgacga atacgccggg ctcaaccaac cagaacgcgt
     2461 ggcgtgatga aaatgcgttc accaaagagg cacggaggac acagaggtcc gatcggcggg
     2521 cgttttgacg atccatgcca gcttcatacc gttcgcattt ctctgtgccc tctgtgtctc
     2581 tgtggtgaaa aacccgcacg cacccgatga acgacgtgat caccgccatc cgtgtcgtgc
     2641 cgctggagca ctacgtggtg ctcagcatga tcctgttcag catcggtctg gcgggtgtgc
     2701 tcttccggcg caacacgatc atcctgttga tgtgcatcga gctgatgttc aacagcgtga
     2761 acctgctgct cacggcgttc agcgcgtacc acagcgatcc gggcggtcag gtcttcgtgt
     2821 tcttcatcat gctggtggcg gcggccgagg tcaccgtggg cctggccatc ctggtgatga
     2881 tgaagcggaa cgtggacagc gtggacatca accaactcag ccgcttgaag ggatgacacg
     2941 ggatatcctg gccaccttgg tcccggcact gccgctcgct ggcgcgatcc tggtcgccgc
     3001 gctccgccgc aagctgaagg gcaactccgc gggcatcctg gccaccctga tggtcgcggg
     3061 gtcgttcgtt tgcagtgcca tgctcttcat cgggcaggag ttcgtcagtg tgatgcacgc
     3121 ggcgaacatg catcagcagg atgcgcagga aggcggaacg cacatcgtga aactcttcga
     3181 ctggatcaac gtgagcacga tgaaggtgcc tttcgcgttc cagatcgacg cgctatcgat
     3241 caccatgatg ctcatcgtca ccggtgtcgg ctccttgatc cacctgtact ccatcggcta
     3301 catgcatgat gacgagcgcg tggccagttt cttctcgatg ctcaacctct tcaccttcgc
     3361 catgctgctg ctggtgatgg gcgcgaactt cgtggtcacc tacatcggtt gggagggggt
     3421 gggcctgtgc agctacctgc tcatcggctt ctggtacagg acgcccgcgt acaactacgc
     3481 cgcacgcaag gccttcgtga tgaaccggat cggcgacctg gggttcgtga tcgggttgat
     3541 gtggctgttc agcacgttgg gcacgctcaa ctacaccgag gtgatggcac gggccggctc
     3601 gctcagtacc gaagcggtca ccgggatcac actcctgttg ttcgtgggcg ccattggcaa
     3661 gagcgcgcaa ttgccgctgt tcacctggct gcccgatgcc atggccggtc ccacgccggt
     3721 gagtgcgctg atccacgccg cgacaatggt aacggcaggt atatttcttc tggtgcgcag
     3781 cagcgcgctg ttcatgttcg caccgttcac ccaggacgtg gtgctctgga cgggcctgat
     3841 cacctcgttg ctcacggcca gcatcggcgt gttccagaac gacatcaaga aggtgctggc
     3901 gtactccacc gtctcgcaac tgggcctgat gttcgtggcc ctcggtgtgg gcgcgtacag
     3961 cgcggccatg ttccacgtca ccacgcacgc gttcttcaag gcgctgctgt tcctgggtgc
     4021 gggcagtgtg atccacgcgc tgggcggcga gcaggacatc cgcaagatgg gcggattgaa
     4081 aggggtgacg aaggtgacct acgtgacctt cctgatcggc acgctggcga tcgcgggcat
     4141 tccgggactg agcggcttct tcagcaagga cgccatcctg gcggcggcct acgaggcgca
     4201 tcccgccatc tgggcggtgt cgttgttgat ctcgctgctc acctgcttct acatgttccg
     4261 cctgctcttc ctcaccttct tcggtagcta ccgtggcaag gcgcatccgc atgagagtcc
     4321 actggtgatg accctgccgc tgatggtgct cgccgtgctc agtgtgttcg gtgggctgct
     4381 caacatcccg catctgttcg gcgggcacga gtcgatgaag catttcctgg ccacggcggc
     4441 cgaaggcatc agcggcgagc acctcaccct ttcccacacc acggagtgga cgttaatggg
     4501 cgtaaccacg gccctgatcg cgctgacgat cgtactggcg tacaaccgtt tcgtgaagcg
     4561 gaccacgctc gatcccgagc ccgaaaaact accgctgctc caacgcctga tcgcgcacaa
     4621 gtggctcgtc gacgaactct acgcgaagct cttcgaacgg ccgtacggct ggctcagcgc
     4681 acacttctac agcattgccg agcgccagtt catggtgccg ctgatggtgg gcaccggtgg
     4741 tgtgacgaac aaggtgggca aggcgttgcg acgcctgcag agcggcaaca tgagcttcta
     4801 tcttttcggt atggtgatcg gcctgatcgc cctgctggcc tggaccctgt acaaggtgtg
     4861 acatgatcct gctcgcactc atagccgtac cgttcctcgc aggcctgctg ttgctggtcg
     4921 cacgccccaa tgcacaggcg cggtccatcg ccatggtggc gtcgctcacc tgcaccgtga
     4981 tcgtgctggc gcaatgctgg aatttcggcg gtgctgaact gaccttcatc gaccgggact
     5041 gggtgccgga ttggggcctg cgactcaaga tgggctacga tggcatcggc ctgctgatgc
     5101 tgatgctcac ggcggtgatc ttccccttca tcatcggagc cggagcgaaa gaggagcagg
     5161 atcggccagc gttggcgaac gcgctgctgc tgttcaccca gagtttcctc ttcggcgtgt
     5221 tcaccgcgca gaacgccctt ctcttctaca tccactacga gttgagcctc attccgctgt
     5281 tcctgtatct gctctactgg ggcacgcctt cgctggaagg ctccagcggc cgaggtgggg
     5341 cggaccgacg gaccattacg atgcgcttct tcatctacac gctcttcggt ggtctggcgc
     5401 tcctgttcgg tatcgcgtac tgtgtggtgc aggagccatc gcacagcgcc gacttcagcg
     5461 ccctgcatgc gttgcggctg ccgatcgatg tccaggtctg gttgttctgg gcactcttcc
     5521 tggccttcgc cgtgaagctg ccgatcttcc cgttccacag ttggcagccc gacacgtaca
     5581 ccatggcgcc gatgcagggc accatggtcc ttggtggcgt gctgttgaaa atgggcctct
     5641 tcggcgtgct gcgcttcgtg ttcccgatcg tgccacaagg tacggatcac tggcgcgacc
     5701 tggtggtgtg gctcagcatc atcggggcca tctacgccgg gtggatcgct ttccggcaga
     5761 ccgacttcaa gcggctggtg gcctactcgt cgttgagcca cgtgggcatc atgacggccg
     5821 cgctgttcac gtggaccaca tacggtatca cgggttcatt gtaccaggcg ttcgcgcacg
     5881 ccgtactggt agtgtgcatg ctctacatcg tgggtgcggt cgaacggcgc atgggtacca
     5941 gggacctctc ggtaatggcc ggcctgaagt tgaagtcgcc gcgactggcc gcgctcttct
     6001 tcctggtgat ggtgaatggg atcgcgctgc ccctgacaca gagcttcgtg ggcgaatggc
     6061 tcatgttcaa cggcctgtgg cagatggagg gcggcgcgtg gatgaccgcg gtggccgtgt
     6121 gtacgatcat cctcggtgcg gtgtacatgc tgtacgcgta ccagcgcgtg atgctcggtc
     6181 ccgagacccg cgtggccccc ggcacatcgc tgaacgaggt tgcggaagcc gacgccaacg
     6241 atcacctctt cctggtgccg ctgatcgcga tcacccttgt gttcggttcc tatcccgcac
     6301 cggtgctgga cctggtgcag agctccgttc aaaccctcct gagcaccctg aattgacatg
     6361 agctcgctca tcctcctttc cgtgctcggc gtgctgctgc tgtatgtggg ccttttcggc
     6421 gcgggcgata aggcgggtgc cagcagggtg cttgcgccca tcgcggtgat cggagtgtgc
     6481 ggcgcgatcg ccctgcttgt gcgcgactac caagtgaaca tccccgattt cgcgcatatg
     6541 ctccagtggg acgatacggg ccgcctgttc agcatcggga tcctgggcat caccgcactc
     6601 atcttcctgt tcggtatgga ctactacgcg aaggaggagc gggacgtggc ggagcaattc
     6661 gcactcatcg ccttcagcct tgtgggggcg ctgatgctca ccagttacac gaacctactc
     6721 gcactcttcc tcggtatcga agtgctgagc atcccgctgt acatcctggc cggaggcaag
     6781 cggcacagct tccgcagcag cgaggcgagc ttcaagtact tcctcatggg ctccttcgcc
     6841 agcgccttcc tgctgatggg catcgcactg atctatgggg tgtgcgcgtc gttcgagctg
     6901 tggaggatca cggaggtggc cgcaacctcg gacaagaaca tcctcttcat tctcggcggc
     6961 ttcttcgtgc tggtgggcct caccttcaag gtagcggcgg caccgttcca cttctggagt
     7021 ccggatgtgt atgaaggggc ccccacgctg gtaacggcct acatggccac cgtggtgaag
     7081 atggccgcct tcgccggtct gttgcgcttc gtatcgatga gcggcatgcc catggccgtg
     7141 ggctacgcgc tggtggtgat ggcggcggcc accttgctcg tgggcaacat cgtggcgttg
     7201 cggcagagcc acttcaaacg gctgatggcg tacagcagca tcgcgcacag tggtttcctg
     7261 ttgatggcca tcgtcagtgg cacgccggac agggatgccg cgctgttcta ttacacgttc
     7321 acctattcca tcgccacgct cggcctcttc atggtgatga ccatcgccaa gcgtgcggcc
     7381 aacggcgacg agcacatccg catcttccgt ggcctcttcc gtgccaaccc ttggatggga
     7441 ctggtcgtgc tgctgctgct gctctccctg gcgggcatcc cgctcacggc gggcttcatg
     7501 gccaagttca aggttttcca gatcgcactg gccatgggtc atgtgaagct cacggtgctg
     7561 gccgtggtga tggccctggt gggcatatac tactatgccg tggtgatccg tgaggccttc
     7621 acccctgcgg aggagacacc tgccctcgtg gtctcaccgc tcaacctcct ggtgatctcc
     7681 ctgtgcggga tcgcggtggt ggtgctgggg gtgatgccgg ggtgggtat
//
LOCUS       DAOQOC010000007         7412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3778_length_7412_cov_3.642789, whole genome shotgun sequence.
ACCESSION   DAOQOC010000007 DAOQOC010000000
VERSION     DAOQOC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3778_length_7412_cov_3.642789"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="gldL"
                     /locus_tag="PL106_00205"
     CDS             complement(<1..440)
                     /gene="gldL"
                     /locus_tag="PL106_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNA31513.1"
                     /translation="MRPGSKAWKNFMAKLYGIGAAVVIVGALFKIQHWPLADVFLILG
                     LSTEAIIFMFSAFEPPHEDPDWSLVYPELATGEHAAGDDFKKEDQRSITEQLDDMLES
                     AKIEPELIESLGNGMRTLSDQARQLTDISSAATATNDYVTNLKGA"
     gene            complement(544..1905)
                     /locus_tag="PL106_00210"
     CDS             complement(544..1905)
                     /locus_tag="PL106_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNA31514.1"
                     /translation="MNLRPIAYLGAVGLLASCGRGGQGQLIGAQNRPAWYQVDPYGMN
                     YIPMGSYVMGPSDQDAPNALVAKSRTVSVQAFYMDQTEISNNEYRQFVEYVQDSIMKR
                     LLGDADIGEHVLTEDEFGEEIDPPVINWNERVDHDDEEVREALAEMYYQSSEQFYRRQ
                     EIDTRKLLFEYYWIDLQEAARKAGRDQDLNAGGYTNSKGQNFSIMGHSDRSKFIIKEV
                     INVFPDTLTWVHDFTYAYNEPMTKNYFWHPAYDEYPVVGITWQQANAFNVWRTQNMMN
                     AWLQQGGEPFINDFRLPTEAEWEYAARGGLKLSAYPWGGPYIRNRQGCFLGNFKPLNG
                     NYTDDGGFHTVPVDSYSANDYGLYNMAGNVAEWTSTAFDESVYDFDHDLNPEYRYDAL
                     ANDPPSLKRKVIRGGSFKDVGLYLQTGTRCFEYQDTAKCYIGFRSVLTYLGRGKAVSA
                     EDL"
     gene            complement(2029..2964)
                     /locus_tag="PL106_00215"
     CDS             complement(2029..2964)
                     /locus_tag="PL106_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA31515.1"
                     /translation="MRGKLTAVVCAVVATVSYAQQDPQYTQFMFDRLSVNSGVAGTSG
                     NLCATLLLRQQWSGFDGAPKTGLLNLQGAIPKINSGVGLSVYFDKLGQENSTIARLHY
                     AYHVKLGGGSSVLGVGLYAGLSSRTLGSQWVAVDPVTEDDAIPDNGSSASGFDLGAGI
                     YYMNKRLWAGLSSTQLPETELKNVSIKNKRHYYAQAGYRADLGKNFALLPSLLIKSDA
                     TSTQFDLNITAMYKDMVWLGATYRTEDAIAPMIGFQMPTGKEKKSMFKIGYSYDVTTS
                     ELRNYSSGSHEIMLNYCFMIKKKEVIEIYGHPRFL"
     gene            complement(3124..4293)
                     /locus_tag="PL106_00220"
     CDS             complement(3124..4293)
                     /locus_tag="PL106_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNA31516.1"
                     /translation="MDISIYFKPSARFGQSRPDWLAHTLGDNTRFHTVSGLPDLEGAQ
                     VAILGVLDDPGHARPRGCAQAPDAVRDELYRLHVPQARVNVVDLGDIHPGHTAKDTQH
                     ALAESIAELIRRNIIPIIIGGGQGHTFSQYLAYEKLERTANLACVDGRFDLGEPGQGL
                     ADSSYLSHMVLRQPNYLFNYSNLGFQTYLVDQPGIELMEKLYFDTHRLGELRANLAEA
                     EPVLRNADTVSVDMSSVRWADAPGTTRPGPNGFHGEEICRIMRYAGVSEKVTSVGIYE
                     MDPIRDQHGITAQLVAQMVWCFLDGVGSRTNDLPWLDRKRFTRFRVPIKGHEQELVFY
                     KSSVSDRWWMDIPYRAEQEARFERHHLVPCAYSDYQMACREEVPDRWWRTYQKLA"
     gene            complement(4395..6506)
                     /locus_tag="PL106_00225"
     CDS             complement(4395..6506)
                     /locus_tag="PL106_00225"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNA31517.1"
                     /translation="MDGSVGRAIEQAADAVLQFLHDARRVFHEREREILGFTEKSAFR
                     VTANLLANGKSVVKAELPKRFDTEAEALAFLQGCIGASFTVNAVEKKPGKRSPAAPFT
                     TSTLQQEASRKLGFGVDRTMRIAQGLYEQGHITYMRTDSVNLSDLAVDAAKAAITSQY
                     GERYSKPRKFANKSKGAQEAHEAIRPTDLNVRIAGSDRDAERLYELISKRTLASQMAE
                     AELEKTVVNIAISTRPGENLVAQGEVILFDGFLKVYMEGKDDEEGEEQEGLLPDMKQG
                     DQLALQDLTATQRFDRPAPRYTEASLVKKLEELGIGRPSTYAPTISTVQKRGYVVKES
                     REGTPRAYRILTLAHGSIAEQTASENTGAEKMKLFPTDIGMVVNDFLVEHFPRIVDLN
                     FTAYVEGEFDQIAEGTMNWRDMIAEFYKPFHVTIGQVKDTAERATGARVLGKDPASGK
                     DVVARIGRFGPMIQIGSADDEDKPKFASLRREQSIQSITFEEAMDLFKLPRTLGERDG
                     ETVTAGIGRFGPFVKWGSTYASLTREDDPLSITLQRAIELIDLKKAASATTELGEFQG
                     EPIVTGRGRFGPYVKHKGLYANIPRSEDPAAITFERAVELIQAKQATAAANTLKTFEG
                     SAIQILKGRYGPYITDGKKNVNVPKDKKPEDMTLEECTALLGGAKAGKGAKTKKTAAP
                     KTAAKKAPKKKAAKRAKAKKA"
     gene            6444..>7412
                     /locus_tag="PL106_00230"
     CDS             6444..>7412
                     /locus_tag="PL106_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNA31518.1"
                     /translation="MKELEYRIGCLFDRAADGAIHQKAFAGQSFDRTVHVGDLTGIEI
                     MSRLRDQIFASNIRCLEYTRGLELLTSADGQRVIGAVLLDTQSGEFITVRSKAVILCT
                     GAGPLMYQRAACAQEKAMDGVAMAYRVGAHLMDMEMVQFHPTGLVVPGSRLNGALLEE
                     GLRGAGAHLFNGQSERFMERYDPEKKERSTRDRVSRASYMEVMAGRGTQNGGVWIDVS
                     HLGAEFVEKNFTGMRDRCLRIGFDLARERVEVCPTAHFNMGGIRINRDCFTNLEGLFA
                     AGEDSAGVHGANRLGGNGVAESTVFGARAGDVVAGWVKHRQHTDEAS"
BASE COUNT         1245 a         2236 c         2329 g         1602 t
ORIGIN      
        1 gcacctttca ggttcgtcac gtaatcgttg gtggccgtag ccgcgctgct gatgtccgtg
       61 agctgtcggg cctgatcgct cagggtacgc atcccgttac cgaggctctc gatcaattcg
      121 ggttcgatct tggcgctttc cagcatatcg tccagctgct cggtgatgga gcgctggtcc
      181 tctttcttga aatcgtcgcc agcggcatgt tcaccagtgg ccagctcggg gtaaaccaag
      241 ctccaatcgg gatcctcgtg cggaggttcg aaggccgaga acatgaagat gatcgcttcc
      301 gtggaaaggc cgaggatgag gaacacgtca gccagtggcc agtgctggat cttgaagagg
      361 gcgcccacga taacgacggc ggcgccgatc ccgtagagtt tggccatgaa gttcttccat
      421 gctttgcttc ctggtctcat cgtagttcct tgcgttaggt ttggttgctg ggtggaattg
      481 gagcggatgc ccgggtccga atgttcaagc gggcgtcagg tttcgtaggt tgtgaaatgc
      541 gcgctaaagg tcctcggcac tcaccgcctt gccgcggccc aggtaggtga gtacggagcg
      601 gaagccgatg tagcacttgg cggtgtcctg gtactcgaag cagcgcgtac cggtctgaag
      661 gtaaaggccc acgtccttga aggaaccgcc acggatcact ttccgcttga gggaaggcgg
      721 gtcgttggcg agcgcgtcgt aacggtactc ggggttcagg tcgtggtcga aatcgtacac
      781 gctctcgtcg aaagcggtac tggtccactc ggccacgttg ccagccatgt tgtacaggcc
      841 gtaatcattc gcgctgtagc tgtccaccgg tacggtgtgg aagccgccat catcggtata
      901 gttgccgttg aggggcttga agttccccag gaagcagccc tggcgattgc ggatgtacgg
      961 accgccccaa gggtaggcag agagtttcag accaccgcgc gccgcatact cccactcggc
     1021 ctccgtgggc aggcggaaat cgttgatgaa gggctcaccg ccctgctgca accaggcgtt
     1081 catcatgttc tgcgtgcgcc acacgttgaa tgcgttggcc tgctgccagg tgatgcccac
     1141 cacgggatac tcgtcgtagg ccggatgcca gaaatagttc ttggtcatcg gctcgttgta
     1201 cgcataggtg aaatcatgca cccacgtgag cgtgtccggg aagacgttga tcacctcctt
     1261 gatgatgaac ttgctccggt cgctgtggcc catgatggag aagttctggc ccttgctgtt
     1321 ggtgtatccc cccgcgttca ggtcctgatc gcggccggcc ttgcgggcgg cttcctgcag
     1381 gtcgatccag tagtactcga aaagcagctt gcgcgtgtcg atctcctggc ggcggtagaa
     1441 ttgctcgctg ctctggtagt acatctcggc cagggcctcg cgcacctcct catcgtcgtg
     1501 atcgaccctt tcgttccaat tgatgacggg cggatcgatc tcctcgccga actcgtcctc
     1561 ggtgagcacg tgctcaccga tatcggcatc acccagcagt cgcttcatga tggagtcctg
     1621 cacgtattcc acgaactggc ggtactcgtt gttgctgatc tccgtctggt ccatgtaaaa
     1681 ggcctggaca gacacggtgc ggctcttggc caccaaggcg ttcggagcgt cctgatcgct
     1741 gggccccatg acgtaactgc ccatggggat gtaattcatc ccgtacgggt ccacctggta
     1801 ccaggccggg cggttctggg cacctatgag ttgcccctga ccaccccggc cacagctcgc
     1861 cagcaagccg acagcgccca aatatgcaat gggacgaagg ttcatggtct tccttttttg
     1921 ccgagttccg tcgcaggtcc ttgcagggtc ctggtctttg ccgccgggta agcggacagg
     1981 ctcagacaag acatcaaacg gcggtttggt tccaattgtt acccgagact agaggaagcg
     2041 tgggtggccg tagatctcga tcacctcctt cttcttgatc atgaagcagt agttgagcat
     2101 gatctcgtga ctaccactgc tgtagttcct caattccgaa gtggtcacat cgtaactgta
     2161 gccgatcttg aacatgctct tcttctcttt gcccgtgggc atctggaagc cgatcatggg
     2221 tgcgatcgca tcctcggtcc tgtacgtggc gcccagccat accatgtcct tgtacatggc
     2281 ggtgatgttc aggtcgaact gggtggaggt ggcgtcactc ttgatcagca aggaagggag
     2341 cagggcgaaa ttctttccaa ggtcagccct gtagcccgct tgggcgtagt agtgtcgctt
     2401 gttcttgatg ctcacgttct tcagctcggt ttcaggcagt tgtgtgctgc tcaggccagc
     2461 ccaaaggcgc ttgttcatgt aatagatgcc cgctccgagg tcgaagccgg aggcactgct
     2521 gccattgtcg ggaatggcgt catcttcggt aacgggatcc acggccaccc attggctgcc
     2581 caaggtcctg ctgctgaggc ccgcataaag ccccacaccc agcacacttg aaccgccacc
     2641 caatttaacg tggtaggcat agtgaagtct agcgatggtg ctgttctcct ggcccaactt
     2701 gtcgaaataa accgagaggc ccaccccgct gttgatcttg gggatggcac cctgaaggtt
     2761 caacaggccg gtcttggggg ccccatcgaa cccgctccac tgctggcgca gaaggagggt
     2821 ggcacagagg ttgccggaag tgccggccac gcccgagttg accgaaagcc ggtcgaacat
     2881 gaattgtgtg tactgcggat cctgttgggc gtatgacacc gttgccacca ccgcgcagac
     2941 cactgcggta agtttccccc tcatgctggt tctcttcaat tggcgcggct ttccggggga
     3001 caaagggtcc ctccggccgg ttccgatccg ccgccaaagt aaaaaaaaag gtgtgccttc
     3061 gatcttttca acaaaaagcg tgcgtcgggg aacgctcgtt caccgtcgtg accgttcccg
     3121 aactcaggcc aacttctggt aggtacgcca ccaacggtcc ggcacctctt cgcggcaggc
     3181 catctggtaa tcgctatagg cgcagggcac caagtgatgg cgctcgaacc gggcttcctg
     3241 ctcggcgcgg tacgggatgt ccatccacca gcggtcgctg acgctgcttt tgtagaagac
     3301 cagttcctgt tcgtggccct tgatgggcac acggaagcgg gtgaagcgct tgcggtcgag
     3361 ccagggcagg tcgttggtgc ggctgcccac cccatcgagg aagcaccaga ccatttgcgc
     3421 aacgagttga gcggtgatgc cgtgctggtc gcggattggg tccatttcgt agatgcccac
     3481 ggaggtcacc ttttcgctca cgccggcata gcgcatgatg cggcagatct cctcaccatg
     3541 gaagccgttc ggcccgggcc gcgtggttcc gggcgcatcg gcccaacgta cgctgctcat
     3601 gtccacactc accgtatcgg cattgcgcag caccggttcg gcctctgcca agttggcccg
     3661 gagctcgcca agccggtgcg tgtcgaagta cagtttctcc atcaactcga tgcccggctg
     3721 gtccaccagg taggtctgga agcccaggtt gctgtagttg aacaggtagt tgggctggcg
     3781 cagtaccatg tggctcaggt agctggaatc ggccaggccc tgcccgggtt cgcccaggtc
     3841 gaagcgaccg tccacgcacg cgaggttggc ggtgcgttcc agcttctcgt aggccagata
     3901 ttggctgaac gtgtgtcctt gcccgccacc gatgatgatg gggatgatgt tgcgccggat
     3961 cagttcagcg atggactcgg ccagtgcatg ctgggtgtcc ttggcggtgt ggcccgggtg
     4021 gatgtcgccc aggtccacca cgttcaccct ggcttgcgga acgtgaagcc ggtagagttc
     4081 atcacggaca gcatcggggg cttgggcaca gcctcgcggt cgggcgtggc ccggatcatc
     4141 cagcactccg aggatggcta cctgcgcccc ctccaggtcg ggcaggccgc tcacggtatg
     4201 gaagcgcgta ttgtcgccca gggtgtgggc cagccaatcg gggcggctct gaccgaagcg
     4261 tgcggaaggc ttgaaataga tgctgatgtc catggcggga agaccttgcg ggccgtcggc
     4321 caaaagtggc ccggcagccg gtcccctcaa cccaccgatg tcggagcctg ttgacagggc
     4381 ggtgttcgca gcgctcaggc ctttttggcc ttggcccgtt tggcggcctt cttcttgggc
     4441 gccttcttgg ccgcggtctt cggtgcggcc gttttcttcg ttttggcgcc cttgcccgcc
     4501 ttggccccgc ccagcagcgc agtgcattcc tccagggtca tgtcctcggg cttcttgtcc
     4561 ttgggcacgt tcacgttctt cttgccgtcg gtgatgtacg gaccataccg acctttcagt
     4621 atttggatgg ccgacccctc gaaggtcttc agggtattgg ccgccgcagt ggcctgcttg
     4681 gcctggatca gttcgacggc ccgctcgaac gtgatggcag cagggtcctc cgatctcggt
     4741 atgttcgcat acaggccctt gtgcttcacg tacgggccga aacgaccgcg ccccgtgacg
     4801 atgggctcgc cctggaattc cccgagttcg gtggtggcgc tggcggcctt cttcaggtcg
     4861 atcagttcga tcgcgcgttg cagggtgatg ctcaggggat cgtcctcacg ggtgaggctg
     4921 gcgtaggtgc tgccccactt cacgaagggc ccgaagcgac cgatgcccgc cgtgacggtc
     4981 tcgccatcgc gctcgcccag tgtgcgtggc agtttgaaca ggtccatggc ctcttcgaag
     5041 gtgatgctct ggatgctctg ttcgcgccgc aggctggcga acttcggttt gtcctcgtcg
     5101 tccgcgctgc cgatctggat catggggccg aaacggccga tgcgggccac cacgtccttg
     5161 ccgctcgccg gatccttgcc caatacccgg gctccggtag cgcgctccgc cgtatccttc
     5221 acctggccga tggtcacgtg gaacggcttg tagaactcgg cgatcatgtc gcgccagttc
     5281 atggtgcctt cggcgatctg gtcgaactcg ccctccacgt aggccgtgaa gttcaggtcc
     5341 acgatgcggg ggaaatgctc caccaggaag tcgttcacca ccatgccgat gtccgtgggg
     5401 aagagcttca tcttctccgc gccggtgttc tcgctggcgg tctgctctgc aatggaaccg
     5461 tgggccaagg tgaggatgcg gtaggcgcgc ggcgtgccct cgcggctctc cttcaccacg
     5521 tagccgcgct tctgcacggt actgatggtg ggcgcatagg tggacgggcg accgatgccg
     5581 agttcctcca gcttcttcac caggctcgct tccgtgtagc gcggtgccgg gcgatcgaag
     5641 cgctgtgtgg ccgtgaggtc ctggagcgcg agctgatccc cctgcttcat gtccggcaga
     5701 agcccctcct gctcctcgcc ttcctcgtcg tccttcccct ccatgtacac cttgaggaag
     5761 ccgtcgaaga ggatcacttc gccttgggcc acgaggttct cgccaggtcg tgtactgatg
     5821 gcgatgttca ccaccgtctt ctcgagttcg gcctcggcca tctggctggc gagcgtgcgc
     5881 ttgctgatca gctcgtacag gcgctccgca tcacggtccg aacccgcgat acgcacgttc
     5941 aggtccgtgg ggcgtatggc ttcgtgcgcc tcctgcgccc ccttgctctt gttggcgaac
     6001 ttccgcggct tgctgtagcg ctcaccgtat tgcgaagtga tggcggcctt ggcggcatcc
     6061 acggccaggt cgctcaggtt caccgagtcg gtacgcatgt aggtgatgtg cccctgctcg
     6121 tacagaccct gcgcgatgcg catggtgcgg tccacaccga agcccagctt gcgcgaggcc
     6181 tcctgttgca aggtggaggt ggtgaacggt gccgccggag agcgtttacc gggcttcttc
     6241 tccaccgcat tcacggtgaa ggacgcgccg atgcacccct gcaggaaggc caatgcctcg
     6301 gcttcggtat caaaacgctt gggcagctcg gccttcacca cgctcttgcc attggccagc
     6361 aagttggccg tcacgcggaa ggccgacttc tcggtgaagc ccaggatctc gcgctcacgc
     6421 tcgtggaaga cgcgccgcgc atcgtgaagg aactggagta ccgcatcggc tgcctgttcg
     6481 atcgcgcggc cgacggagcc atccatcaaa aggcgtttgc gggtcaaagc tttgatcgca
     6541 cggttcacgt cggcgatttg accggcatcg aaatcatgtc gcggctgcgc gatcagattt
     6601 ttgccagcaa catccgctgc ctcgaataca cgcgcggctt ggaattgctc acgtccgccg
     6661 acggccagcg cgtcatcggc gcggtgttgc tcgatacgca aagcggcgag ttcatcaccg
     6721 tgcgcagcaa ggcggtgatt ctctgcaccg gcgccggacc gctcatgtat caacgcgccg
     6781 cgtgcgcgca ggagaaagcc atggacggcg tcgcgatggc gtatcgcgtc ggcgcacatc
     6841 tcatggacat ggagatggtg cagtttcatc cgaccgggct cgttgtgccc ggctcgcggc
     6901 tcaatggcgc gctgcttgaa gaaggtctgc gcggcgcggg cgcgcatttg ttcaacggcc
     6961 agagcgagcg tttcatggaa cgctacgatc cggaaaagaa ggagcgctcc acgcgcgatc
     7021 gtgtttcgcg cgcgagttac atggaagtga tggcgggccg cggcacgcaa aacggcggcg
     7081 tgtggattga tgtctcgcat ctcggcgcgg aattcgtgga gaaaaatttc accggcatgc
     7141 gcgaccgttg cctgcgcatc ggcttcgacc ttgcgcgcga acgcgtggag gtttgcccga
     7201 ccgcgcactt caacatgggc ggcattcgca tcaatcgcga ttgtttcacg aatctcgaag
     7261 gcctgttcgc cgccggcgaa gattcggccg gcgtgcacgg cgcgaatcgt ctcggtggca
     7321 acggcgtggc ggaatccacc gtgttcggcg cgcgcgcggg cgatgtcgtc gcgggttggg
     7381 tgaagcatcg ccagcacacc gacgaagcca gc
//
LOCUS       DAOQOC010000008         7274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3882_length_7274_cov_4.651475, whole genome shotgun sequence.
ACCESSION   DAOQOC010000008 DAOQOC010000000
VERSION     DAOQOC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_7274_cov_4.651475"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PL106_00235"
     CDS             <1..310
                     /locus_tag="PL106_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNA31519.1"
                     /translation="WFSEWENGANVYRDPAKYDVFNPALHAKDWSVPMLIIHGDLDYR
                     IPVEQGIGAFTALQAMGVDSRLLRFADENHWVLKPRNSMQWHNTVFGWLEKHIGKGGR
                     "
     gene            410..1876
                     /locus_tag="PL106_00240"
     CDS             410..1876
                     /locus_tag="PL106_00240"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNA31520.1"
                     /translation="MRTPLLLFIVLLAACGRAPSHTEQTVAKPEPTGTANLAYKWGGV
                     ALWAMADETDLRGPRPTIGSRLLAIPMIAQFDAWSRYDSVAVPVHLDAPRAAVPERTL
                     ANKEKAISYAMARALQSAFPHDSALFADSLRAFGYDPADFSLDPATPQGIGNLAAKAV
                     IDARRNDGSNMLGEDPRAPVPLGDYTHYVPKNPVEKMNDINRWQPKYFTKDDGTKWAP
                     GALTPHWGHVRCFALDSSSQFRCPPTPRMGDPALEKEVRQVVAVQAGLTNAEKALVEF
                     MRDGPRSVQQAGHWLLFSRDVSVRDKHTLDDDVKMYFIVTTTAMDCFIACWESKFFYD
                     TSRPYQQVHYLMGNEDIMGWPGPHKGMVKMKGKDWRPYSPDSFLCPPFPSYPSGHSTV
                     SGGCAEVLKLYTGSDDYGFQVRLLPGWLTEPGITTDSITLDFPTFTSTAEQAGWSRVL
                     GGYHIQADNVEGLALGRKVGAVVYDKCMGYIRGTAKRK"
     gene            2012..2431
                     /locus_tag="PL106_00245"
     CDS             2012..2431
                     /locus_tag="PL106_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31521.1"
                     /translation="MDRLRHVLYLLALLTGIRSSAQAVIGDSTYLRIPAQAKAYESMR
                     FDMDAYRDTLRVFRDAIETTATGINAIDKAGQEQKAKLGRLRVDVKMLDKEMKRQRKK
                     LKRPQPLFEQTAKDCSKLVQRSKWLRTQLKEQGVRLR"
     gene            complement(2438..2851)
                     /gene="ybeY"
                     /locus_tag="PL106_00250"
     CDS             complement(2438..2851)
                     /gene="ybeY"
                     /locus_tag="PL106_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNA31522.1"
                     /translation="MITFLATGVPSVFRDRTRLRKWLTAVAKDHGQSIGELNYVLMSD
                     LALLKFNKEFLQHDDFTDVITFDGGQDRKLVNGDVLMSLDRITDNAKTFGVSRQHELR
                     RVMVHGLLHLLGHTDKKPSQRKAMSALEDKYLARW"
     gene            complement(3016..3327)
                     /locus_tag="PL106_00255"
     CDS             complement(3016..3327)
                     /locus_tag="PL106_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNA31523.1"
                     /translation="MALRFDGETEVAVAKPVYDLEERLAVFAARIVVYVNRMPGNIAG
                     RYYAGQLLRSGGSPALHYGESQAAESNDDFIHKCKIALKDVEGIAGQSAHSEALQADA
                     R"
     gene            complement(3368..3805)
                     /locus_tag="PL106_00260"
     CDS             complement(3368..3805)
                     /locus_tag="PL106_00260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA31524.1"
                     /translation="MAALTEDVQYTRRIDFPAKAENIALAEKLIDEACQKHGVHESHY
                     GNILIALTEAVNNAIHHGNRLDPSKSVGLGYQVKGDKIVFVVSDEGPGFDFQHLPDPT
                     DPQNIEKPHGRGVFLMRALADSVEFSDNGATVAMAFGLQPVKE"
     gene            complement(3812..7249)
                     /locus_tag="PL106_00265"
     CDS             complement(3812..7249)
                     /locus_tag="PL106_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31525.1"
                     /translation="MPSDYNLLIEKLDAFIRKYYKDRLIRGALYSVGLLVLFFLGASL
                     SEYFGRFGTTARTVLFWGFLLVAAGVLARFIILPLVKLFHLGKVISHDEAAAIVGQHF
                     GEVKDKLLNTLQLREQANSNFVNRELIEASIAQRSRELSPVPFAAAIDLGKNRKYLRY
                     ALPPLAVLLVLLFAAPSLITDPANRIIKHTSEFAPPAPFRFVVLDSNNLNVPEQEDFD
                     LRVAIVDGAVPQQVELDINGQRIPLVKQDASHFIHRFRNVQEAVHFKLTADGFFSKPY
                     TLETVSNPMLTGLAVRMEYPPYLGMPPATANGGGDVTVPAGTRITWAANTRSTDELEL
                     AFDDTTYTLVPQGENAFTASRRFLQGHTYTIVPRSHSPGGGERTAKDPLQYRVDVVPD
                     LYPPILIEQKTDSTSPKRLYFKGDIGDDHGFKRLVFHYGFSQGGDSTAAELRERDQTI
                     GIDPKNTRQQFFHFWDLNDLRLLPGDKLEYWFEVWDNDGVHGSKSARTATQVFEAPTL
                     KELADKQNADNEAVKSELKESIKEAQELQRELDKLRRDMLDKKDLNWQDKQKLENVME
                     KQKQLQQRIEKTTEQFKQNNQEQQSFRDMDERLLQKQQQVQELFENVLSEEMKKLYEE
                     VQKLMEQMDKEDIQEKMEEMKMSQEDIEKELDRAMEQFKRMEVEQKAEDIAEQLEKLA
                     EEQKKLAEETRNEKGDNSEQQKKQEELNKAFDDIRKQLDDLEKKNSELETPVELPKTD
                     QQEQDIQKEQEGSQQDMQQKQNQKAGDKQQKAGEQMEQMAFQMKSAMGANEQQQQEED
                     MDALRQLLENIVQLSFDQEAALADLNRTAAKDPHLVDIGRQQKKMRDDARVVEDSLFA
                     LSKRVPQLQAAVNREMNAVNENMDQAMEHLATSRADLRGKPQAADKQQRAMTGLNNLA
                     LLLDEALQQMQQPPSGMPGSGKCNKPGGKGSSSGNSKKMAQTRANQQALSKQLEEMKK
                     ALEKGGKKPGDKPGEQPGKDGKPGGLGMPGMSQQLAQLAAQQAAIRKEMQRAAQELNK
                     DGTGAGQPLQKLAEEMEKLEKDIVNKNITQESLKRQQDIMVRLLEAEKSERERELDNK
                     RESNEGRETPHADPARFFEYQRTKQREAELLRTVPPGLKPYYKARVAEYFDTFDRPR"
BASE COUNT         1225 a         2172 c         2272 g         1605 t
ORIGIN      
        1 atggttcagc gagtgggaga acggggcgaa cgtttacagg gaccccgcga agtacgacgt
       61 gttcaatccg gcgttgcacg ccaaggactg gtcggtgccg atgctcatca tccacggcga
      121 cctcgattac cggatcccgg tggagcaggg catcggagcg ttcaccgcat tgcaggccat
      181 gggtgtggat tcgcgcttgc tgcgattcgc cgacgagaac cactgggtac tcaaaccgcg
      241 caacagcatg cagtggcaca acaccgtgtt cggctggctc gaaaagcaca tcggcaaggg
      301 cgggcgatag caccggagcg gggatccaca tggctggagg cgccgatcat ccatgatcgg
      361 tcgtgtgccg gatgcggtga actaaactca ccgcccattc aacacgaaca tgcgcacccc
      421 attattgctg ttcatcgtat tgctggctgc ctgcggtcgg gcgccctcac acacggaaca
      481 aaccgtcgcg aagcccgaac ccaccggcac ggccaacctg gcctacaagt gggggggcgt
      541 ggcgttgtgg gccatggccg atgaaacgga ccttcgcgga ccgcggccga ccatcggctc
      601 ccggttgctg gccatcccca tgatcgccca gttcgacgcg tggagccggt acgacagcgt
      661 ggccgttccc gtccacctcg acgcgcccag ggccgcggtt ccggagcgca cactggcgaa
      721 caaggaaaag gccatcagtt atgcgatggc cagggcgctg caatccgcct tcccgcacga
      781 cagcgccctg ttcgccgatt cgctacgagc tttcgggtac gaccctgccg acttcagcct
      841 ggacccggcg acgccccaag gcatcgggaa cctggcggcg aaagcggtga tcgatgcacg
      901 gcggaatgat ggcagcaaca tgctcggcga agacccgcgt gcgcccgtgc cactgggtga
      961 ttacacccat tatgtgccga agaacccggt ggagaagatg aacgacatca accgttggca
     1021 gccgaaatac ttcaccaagg acgatgggac gaagtgggcc cccggagcgc tcacgccgca
     1081 ttggggccac gtacggtgct tcgcgctgga cagcagcagc cagttccgct gcccgcccac
     1141 gccgcgcatg ggcgaccccg cgcttgagaa ggaggtgcgg caggtggtgg ccgtgcaggc
     1201 aggactgacc aatgcggaga aggcgttggt ggagttcatg cgcgatggtc cgcgcagcgt
     1261 gcaacaggcc ggtcattggc tcctcttcag ccgcgacgtg agcgttcgcg acaagcacac
     1321 gctggacgat gacgtgaaaa tgtacttcat cgtcacgacc acggccatgg actgcttcat
     1381 cgcgtgctgg gaatcgaaat tcttctatga caccagccgc ccctaccagc aggtacatta
     1441 cctgatgggc aacgaggaca tcatgggttg gccgggcccg cacaagggca tggtgaaaat
     1501 gaagggcaag gattggcggc cctactcgcc ggacagtttc ctgtgcccgc cgttcccctc
     1561 ataccccagc ggccatagca ccgtgagcgg cggttgcgcc gaggtgctca agttgtacac
     1621 cggtagcgac gactacggct tccaagtaag acttctcccg ggctggctga ccgagccggg
     1681 catcaccacg gacagcatca cgctggattt ccccaccttc acatccactg cggaacaggc
     1741 gggttggagt cgcgtgctcg gtggttacca catccaggcg gacaacgtgg agggcctggc
     1801 cttggggcgg aaggtgggtg cggtggtgta cgacaagtgc atggggtata tcaggggcac
     1861 ggcgaagcgc aagtgagctc gtgaccaacg cccggtcaag ccggatagcg gtgcgggcca
     1921 ctgatgccca accttgcgct ccggatgcga tacgaatagc ggtggtactc cggcagggtg
     1981 tagcttcacg cgcggagaaa cccccgttct gatggaccgg ctacgacacg tcctgtactt
     2041 gctcgcgttg ctcacgggca tcagaagttc tgcccaagcg gtgatcggcg atagcaccta
     2101 cctgcgtatt cccgctcagg ccaaagctta cgagtcgatg cgttttgaca tggatgcgta
     2161 ccgcgatacg ctgcgcgtgt tccgggatgc gatcgagacc acggccacag ggatcaacgc
     2221 catcgacaaa gcgggccagg agcaaaaggc caaactcggg cgattgcgcg tggatgtgaa
     2281 gatgctggac aaggagatga aacggcagcg caagaaattg aagcgaccac agccactgtt
     2341 cgagcagacg gcgaaggact gctccaagct ggtgcagcgc tcgaaatggc tgcgcacgca
     2401 attgaaggaa cagggcgtga ggttgcgctg aacccgccta ccaacgggcg aggtacttgt
     2461 cctccagtgc gctcatcgcc ttgcgctgcg agggcttctt gtccgtatgc ccgagcaggt
     2521 gcagcagccc atgcaccatc acgcggcgca gttcgtgctg cctgctcacc ccgaaggtct
     2581 tcgcgttgtc ggtgatgcgg tccaggctca tgagcacatc gccgttcacc agtttgcggt
     2641 cttgcccgcc atcgaaggtg atgacgtcgg tgaagtcatc gtgttgcagg aactccttgt
     2701 tgaatttgag caatgccagg tcgctcatca gcacgtagtt cagctcgccg atgctttggc
     2761 cgtgatcctt ggccacagcg gtgagccact tgcgcaggcg cgtgcgatcg cggaaaacgg
     2821 aaggtacacc ggttgcgagg aaggtgatca ttcaagcttc aagtcccaaa acgcaatgct
     2881 caagcttcaa catccaagag gtgggcggag gccttgagct accgctttcc gagcttttgc
     2941 cggttgatga tgatcgtggc caggatacga atgagttctt cgcactcctg tgcgagccaa
     3001 accagatcag ggtcatcatc tggcatcagc ttggagagct tctgaatgcg cagattgccc
     3061 cgcgattcct tcaacatctt taagcgcgat cttgcacttg tggatgaagt cgtcgttcga
     3121 ttcagcggct tgcgattccc cgtagtgcaa ggcgggtgaa ccgccgctcc tcaacaactg
     3181 gcccgcgtag tagcgccctg cgatgttccc cggcatcctg ttgacgtaca cgactatccg
     3241 ggcggcgaaa accgccaggc gctcttcaag atcgtagacc ggtttggcca cggcaacttc
     3301 tgtttctcca tcgaaccgca aggccatgtt gggccttgca gcttgaggct tgaattttga
     3361 agcttgacta ttccttcacc ggctgcagcc cgaaggccat ggccacggtg gccccgttgt
     3421 cggagaactc cacgctgtcc gctaaggcgc gcatcaggaa gacaccacgt ccatggggct
     3481 tctcgatgtt ctgggggtcg gtggggtcgg ggaggtgctg gaagtcgaag cccggaccct
     3541 catcgctcac cacgaagacg atcttatcgc ccttcacctg gtagccgagg ccgacgcttt
     3601 tggagggatc caggcggttg ccgtggtgga tggcgttgtt caccgcctcg gtgagggcga
     3661 tgaggatgtt gccgtagtgg ctctcgtgta cgccgtgctt ctggcaggcc tcatcgatga
     3721 gtttttcggc gagcgcgatg ttctcggcct tggccggaaa gtcgatgcgc cgggtgtact
     3781 gcacgtcctc ggtaagagct gccatcgggg gttatcgggg tcggtcaaaa gtatcgaaat
     3841 actccgccac ccttgccttg tagtagggct tcaatcccgg gggcactgtt cgtaacagtt
     3901 cggcctcgcg ctgtttggtg cgttggtact cgaagaagcg ggcggggtcg gcatggggtg
     3961 tttcgcggcc ctcgttgctc tcgcgcttgt tgtccaactc gcgctcccgc tcgctcttct
     4021 cggcttccag caggcgtacc atgatgtcct gctggcgctt gagggattcc tgggtgatgt
     4081 tcttgttcac gatgtccttc tccagcttct ccatctcctc ggccagcttc tgcaggggct
     4141 gaccggcgcc ggtgccgtcc ttgttcagtt cctgtgcggc gcgttgcatc tccttgcgta
     4201 tggcggcttg ttgcgcggcg agttgcgcca attgctggct catgccgggc atgcccaggc
     4261 caccgggttt gccatccttg ccgggctgtt cgccgggttt gtccccgggt ttcttgccac
     4321 ctttctccag cgcctttttc atctcctcca actgcttgct cagggcttgc tgattggcgc
     4381 gggtctgggc catcttcttg ctgttgccgc tgctgctgcc cttgccgccg ggcttgttgc
     4441 atttgccgct tccgggcatg ccgctcggtg gttgttgcat ctgttgcaac gcctcgtcaa
     4501 gcaggagggc caggttgttc agcccggtca tggcgcgttg ctgtttgtcg gccgcctggg
     4561 gcttgccgcg caggtcggcg cgactggtgg ccaggtgctc catggcctgg tccatgttct
     4621 cgttcacagc gttcatctca cggttcaccg cagcctgtaa ttgcggcaca cgtttgctca
     4681 gcgcgaagag cgagtcctcc accacgcggg catcgtcgcg cattttcttc tgctggcggc
     4741 cgatgtccac caggtgcggg tccttggcgg ccgtgcggtt gaggtcggcc agtgcggcct
     4801 cctgatcgaa gctgagttgc acgatgttct ccagcagttg ccgaagcgca tccatgtcct
     4861 cttcttgttg ctgctgctca ttggcgccca tggcgctctt catctggaaa gccatctgct
     4921 ccatctgctc gccggccttc tgctgcttgt cgcccgcctt ctggttctgc ttctgctgca
     4981 tgtcctgctg gctgccctcc tgctccttct ggatgtcctg ttcctgttgg tcggtcttgg
     5041 ggagctccac cggggtttcc agttcgctgt tctttttttc cagatcatcc aactgctttc
     5101 ggatgtcgtc gaaggccttg ttcagttcct cctgtttttt ctgctgttcg ctgttgtcgc
     5161 ccttttcgtt cctggtctct tcggccagct tcttctgctc ctcggccagc ttctccaact
     5221 gctccgcgat gtcctcggcc ttctgctcca cctccatgcg cttgaactgc tccatggcgc
     5281 gatcgagttc tttttcgata tcctcctggc tcatcttcat ctcctccatc ttttcctgga
     5341 tatcctcctt gtccatctgc tccatgagtt tttgcacctc ctcgtagagc ttcttcatct
     5401 cctcgctgag cacgttctcg aaaagctcct gaacctgttg ttgtttttgc aggaggcgtt
     5461 cgtccatgtc ccggaagctc tgctgctcct ggttgttctg tttgaactgc tcggtggtct
     5521 tctcgatgcg ctgctggagc tgcttctgct tctccatcac gttctcgagc ttctgtttgt
     5581 cctgccagtt gaggtccttc ttgtcgagca tgtcgcgccg gagcttgtcg agctcgcgct
     5641 gcagctcctg ggcctccttg atgctctcct tgagttcgct cttcaccgct tcgttatcgg
     5701 cgttctgttt gtcggcgagt tccttcaagg tgggcgcctc gaacacctga gtggcggtgc
     5761 gagcgctctt gctgccatgc acgccatcgt tgtcccatac ttcgaaccag tattccagct
     5821 tgtcaccggg caggagtcgc aggtcgttga ggtcccagaa atggaagaat tgttggcggg
     5881 tgttcttcgg gtcgataccg atcgtctggt cgcgttcgcg cagttctgcg gccgtgctgt
     5941 cgccgccttg gctgaagcca tagtggaaca ccaggcgctt gaacccgtgg tcgtcgccga
     6001 tgtcgccttt gaagtagagg cgcttgggcg aggtactgtc ggtcttctgc tcgataagga
     6061 tgggggggta gaggtcgggc accacgtcca cgcggtactg cagcgggtcc ttcgcggtgc
     6121 gctcaccacc tccgggggag tgggagcggg gcacgatcgt gtacgtgtgg ccctgcagga
     6181 accggcggct ggcggtgaag gcgttctccc cctgcggcac gagggtgtac gtggtgtcgt
     6241 cgaaggcgag ttcgagctcg tcggtgctgc gcgtgttggc ggcccaggtg atgcgggtgc
     6301 ccgcaggcac ggtgacatca ccaccgccat tggccgtcgc ggggggcata ccgagatacg
     6361 gtgggtattc catacgcacg gccaggccgg tgagcatcgg gttgctcacg gtctccagcg
     6421 tgtagggctt gctgaagaag ccgtcggcgg tgagcttgaa atggacggct tcctgcacat
     6481 tgcggaagcg gtgtatgaag tgcgatgcgt cctgcttcac cagcgggatg cgctggccgt
     6541 tgatgtccag ctccacctgc tggggcacgg ccccgtctac gatggcgaca cgaaggtcaa
     6601 aatcctcctg ttcaggtacg ttcaagttgt tgctgtccag caccacgaag cggaacggtg
     6661 ctggcggtgc gaactcgctg gtgtgtttga tgatgcggtt ggcggggtcg gtgatgaggc
     6721 tcggagcggc gaaaagcagc acgagcaaca cggccagcgg aggcagtgcg tagcgcaggt
     6781 atttgcggtt cttgcccagg tcgatcgccg cggcgaaagg aacaggcgaa agctcgcggc
     6841 tgcgctgggc gatactggcc tcgatgagct cacggttcac gaagttgctg ttggcctgct
     6901 cgcgcagttg cagcgtgttc aacagtttgt ccttcacctc gccgaaatgc tggcccacga
     6961 tggcggcggc ctcgtcgtgc gagatgacct tgcccagatg gaacaacttc accagcggaa
     7021 gaatgatgaa gcgggccagc acaccggccg ctaccagcag gaagccccag aagagcaccg
     7081 tgcgcgccgt ggtaccgaaa cgcccaaagt attcactgag cgaagcgccc aggaagaaga
     7141 gcacgagcag ccccacgctg tacagggccc cgcggatcag gcggtccttg tagtacttcc
     7201 ggatgaaggc atccagcttc tcgatcagca ggttgtagtc ggagggcatc tgggttggaa
     7261 gtgccacgtc gagc
//
LOCUS       DAOQOC010000009         7166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_3974_length_7166_cov_3.468710, whole genome shotgun sequence.
ACCESSION   DAOQOC010000009 DAOQOC010000000
VERSION     DAOQOC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_7166_cov_3.468710"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PL106_00270"
     CDS             <1..343
                     /locus_tag="PL106_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNA31526.1"
                     /translation="YTKYKDKGFIIVGFPSNDFGKQEPGDEKSIAAFCEKNYGITFPM
                     MSKVVTKGDGQDPVYQWLTHKDKNGASDNEVRWNFTKFLINEDGSLHTVLGSPTSPLS
                     DQVIEWIEGRK"
     gene            322..1065
                     /gene="bshB1"
                     /locus_tag="PL106_00275"
     CDS             322..1065
                     /gene="bshB1"
                     /locus_tag="PL106_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNA31527.1"
                     /translation="MDRGKEVMAEQVDILCITAHPDDVEISCAGTVLRHVAAGRRVGL
                     VELTAGELGTRGTPEIRAQEAEAARKVLGAEFRYGLGLPDGFFRADEPSLRKVVAAIR
                     RHAPRVVITNAVRDRHPDHGRAAALVAEASFLSGLRRIITTHEGREQPVWRPVSVLHC
                     VQDRWIDPHLVVDVSAFWDLKRQALACFKSQFHDPGSTEPVSPISVPEFLPTLEGRAR
                     DMGRIIGVPFGEGFTCARPPGINDVLDLT"
     gene            1071..1289
                     /locus_tag="PL106_00280"
     CDS             1071..1289
                     /locus_tag="PL106_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31528.1"
                     /translation="MIFHLSFAHSPWIPSPQLPDDPKRLCIVEVHHARFDRKCFELLH
                     WDGLKWRFQDNTALTNNAVVLRWALIES"
     gene            complement(1574..2752)
                     /locus_tag="PL106_00285"
     CDS             complement(1574..2752)
                     /locus_tag="PL106_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNA31529.1"
                     /translation="MTDPRERGASPAGGPGSGARRPSRLLLPLWWLNGLAVIGVLLSQ
                     LSPHVSPVTFWPLAFFGIAWPVLMLLNGVFILIWLILRRKRVWPSVIAMALGAGHLGE
                     YIGLPFRGGPPGTTTSPIKVMSWNTRIFDLYNWSHNVNTKDEMLDLIHMEDADVLCLQ
                     EFVDNDARWGRSVKDELLRDMRFTRVADAYTSHTKHGYHFGIATFSTMPIIAQGAIHF
                     PDDLNNLCLWSDIALKNDTVRVYNAHLASLRFTAAEYNFVKEVQDGTSTEEFEHGGKR
                     LAQRLKNGFLRRASQVDRITAHMRTCPYPIIWCGDMNDTPMSWSYQQLRSVGLKDAFT
                     ESGSGTGNTYLGIFPGVRIDHIMHGPRLRSWGSRTLPDELSDHRAIVCRMDVIKAGEE
                     "
     gene            complement(2775..3677)
                     /locus_tag="PL106_00290"
     CDS             complement(2775..3677)
                     /locus_tag="PL106_00290"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA31530.1"
                     /translation="MAITDDIRAQWRTGGAMKRLLFLNLGVFLGVHLIGLIAWAANGG
                     AYDERVLEWLMATNNWPVLLRRPWTVLTYMFTHFDPFHLFWNMVMFWFSGRLFEDLLG
                     GKRLTGMYLLGGLSGFAFYALASVLPAHLGLGGTSMLLGASAAVMAVFIGIATYHPEM
                     EVSLMFIGGVRLKWVALVVLVLDLISIRQGSNTGGHLAHIGGAAYGYLAMMALKSGKG
                     DWNKSLIQWLEGLLGMLRARGSSRLRVEKRPVRRGVLADADFNAAKHDKQARVDAILD
                     KISRSGYDSLSKDERDFLFHASKD"
     gene            complement(3681..4496)
                     /locus_tag="PL106_00295"
     CDS             complement(3681..4496)
                     /locus_tag="PL106_00295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA31531.1"
                     /translation="MMRYNPSGGVFGGIPTVVKNLLILNGLVMLVKLTGIAGLSIPEF
                     DDLFGLHYPSSPLFRPYQFLTHMFVHAGPMVPGDGWYLHILLNMFGLYMFGPQVEYRW
                     GSQRFLAYYLLCGLGSALLYLGWLWYGSREHFATLGPDELALLRDQFIQWSQGGTITG
                     AITPDMAAILRLWSEPMVGASGAIFGVLLAFGLLFPDVRLMMLFFPVPIKAKYFVVIY
                     AVIELVSGVANRSGDNVAHFAHLGGMVAGFVLLKWFERRDRGNAGHQQDLPWR"
     gene            complement(4493..6313)
                     /gene="mutL"
                     /locus_tag="PL106_00300"
     CDS             complement(4493..6313)
                     /gene="mutL"
                     /locus_tag="PL106_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNA31532.1"
                     /translation="MSDIIRLLPDHVANQIAAGEVVQRPASAVKELLENSVDAGATDI
                     TLVLKDAGRTLIQVTDNGHGMSPTDARLSFERHATSKIRSADDLSAVRTKGFRGEALA
                     SIAAVAQVELKTRRPEDELGTRILIEGSRVKSQETVACATGSVLSVRSLFYNVPARRQ
                     FLKSDPIELKHAIEEFHRVALAHPDIAFDLFHNDQEVHRLPVANLRQRITHLFGRKYD
                     ERLVPVSEATGIVGVEGFICKPEFARRTRGEQYFFVNGRYIRSGYLEHAVRRAYDELV
                     QRDHHPGWFLLLHIDPAFIDINIHPTKTEIKFRDDRAVYAILHAAARRALGRHSITPS
                     LDFEPEPALLAMSGVDTAPVPATGNAGANWRPRDLPRQTIPAGWQRLFDLPDDAPQRS
                     GADPEASPTDTVGGQHVLPMRETMDLGARPVFQLHGRYILAQLRSGFVVIDQRRAQER
                     IVYERSMRSLEKGQGPSQAQLFPVSIGLNAADHALLTGILPELGALGFRLEPLSGRSF
                     AVNGLPAESGDGDPQQLVEQLLEQVKNESGALRSERHATIARGMARSMVRNEGRQWSA
                     TEMHDLIDRLFACGMPYTTPGGKPVLITWSLQELDERFDR"
     gene            complement(6454..6582)
                     /locus_tag="PL106_00305"
     CDS             complement(6454..6582)
                     /locus_tag="PL106_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31533.1"
                     /translation="MPQDSNARLNAKRKATLKLAKRKAAGVTAKATRGKKAAKKKA"
     gene            complement(6692..>7166)
                     /locus_tag="PL106_00310"
     CDS             complement(6692..>7166)
                     /locus_tag="PL106_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31534.1"
                     /translation="GKNSRWPVLTLSPEVHVSWQAGYWERNLALRINRGPAVLGAGYR
                     FGNAITVLAGYETGRMALGLSTDITTARTLTDLPYNHQGAPQGVVQPFTIELAVQWRM
                     RCETSATRRKGIPCPDLTGKHLWPLGRGDKKSDGATPAPGGGSRFEDNVVPDRPR"
BASE COUNT         1298 a         2310 c         2220 g         1338 t
ORIGIN      
        1 gtacacgaag tacaaggaca aggggttcat catcgtgggt ttccccagca acgatttcgg
       61 caagcaggaa ccgggcgacg agaagtccat cgccgcgttc tgcgaaaaga actatggcat
      121 cacctttccc atgatgagca aggtggtgac caagggcgat ggacaggacc cggtgtacca
      181 gtggctaacc cacaaggaca agaacggcgc cagcgacaac gaggtgcggt ggaacttcac
      241 caagttcctg atcaacgagg acggatcatt gcacaccgtg ctcggttcgc caaccagccc
      301 gttgagtgac caggtgatcg aatggatcga gggaaggaag tgatggcgga acaggtggac
      361 atcctctgta tcacggcgca tccggacgat gtggagatca gctgcgcagg cacggtcctc
      421 aggcatgtgg ccgcgggcag gcgtgttgga ctggtcgaac tcacggcggg cgaattggga
      481 acccgtggca ccccggagat ccgcgcgcag gaggccgagg ccgcacgcaa ggtgctcggt
      541 gcggaattcc gttacggact gggcctgccc gatggctttt tccgtgcgga cgaaccgagc
      601 ctgcgcaagg tggtcgccgc gatccgtcgc catgccccgc gtgtggtcat caccaatgcc
      661 gttcgcgatc gccatccgga ccatgggcgg gccgccgcat tggtggctga agcctccttc
      721 ctcagcggct tgcggcgtat catcaccacg cacgaaggcc gcgaacagcc cgtgtggagg
      781 ccggtgtccg tgctgcattg cgtgcaggat cgttggatcg atccgcatct cgtggtggat
      841 gtgagcgcct tttgggacct caagcgccaa gcccttgcct gcttcaagag ccagttccac
      901 gatccgggca gcaccgaacc ggtctcgccc atcagcgtgc cggagttcct gcccacgctc
      961 gaaggtcgtg cccgcgacat gggccgcatc atcggcgtgc ccttcggcga gggcttcacc
     1021 tgcgctcggc caccgggcat caacgatgtt ctcgatctta cctgaaacaa ttgatctttc
     1081 atttgagctt cgcacattcc ccctggatcc catcgcctca actgcccgat gatcccaagc
     1141 gcctctgcat cgtcgaggtc caccatgcca ggttcgatcg caagtgcttc gagttgctcc
     1201 actgggacgg gctcaagtgg cgattccagg acaacaccgc gctgaccaac aacgctgtgg
     1261 tgttgcgctg ggcactcatc gagagttagc cgcacgacga tccggaagcc ggggaccgca
     1321 cgctgttttc ccgtggtcat tgttggtgac cgtgatcgtt cgtccggtca gggtgaacgg
     1381 aaaagcaact ttattcggcc cggcggcaac catccccttc gggttccgtc tatccatcgc
     1441 tcctgaagat cccatgatgc aacgctccac cactgcgaac cctggccgat ccaccctcat
     1501 cctgaccagc ggcatcatct gtgccgtgct cagcgccacg atgttctgcg tggcctatct
     1561 ggtgctctga cgttcactct tcacccgctt tgatcacgtc catccggcaa acgatggcgc
     1621 ggtgatcgct caattcatcg ggcagggtcc tggatcccca ggagcgtaat cgtggcccgt
     1681 gcatgatgtg gtcgatgcgg accccgggga agatgcccag gtacgtgttg ccggttcccg
     1741 aaccgctttc cgtgaacgca tccttgagcc cgacgctgcg caattgctga tagctccaac
     1801 tcatgggtgt gtcgttcata tcaccgcacc agatgatcgg atagggacag gtgcgcatgt
     1861 gcgcggtgat ccggtccact tgggaggcgc gccgcaggaa gccgttcttc aaccgctggg
     1921 ccaggcgttt gcctccgtgc tcgaattcct ccgtcgatgt gccatcctgc acttctttca
     1981 cgaagttgta ctccgctgcg gtgaagcgca agctcgccag atgcgcgttg tacacgcgaa
     2041 ccgtatcgtt cttcaaggcg atatcactcc acaggcacag gttgttcaga tcatccggga
     2101 agtggatggc tccctgcgcg atgatgggca tggtgctgaa cgtggcgatc ccgaagtggt
     2161 aaccatgctt cgtgtgcgag gtgtacgcgt cggccacccg ggtgaagcgc atgtcccgga
     2221 gcaattcatc cttcactgac cggccccagc gcgcatcgtt gtccacgaac tcctgcaggc
     2281 acagcacatc ggcgtcctcc atgtggatca ggtccagcat ctcgtccttc gtattcacat
     2341 tgtggctcca gttgtacagg tcgaagatcc gcgtgttcca gctcatcacc ttgatagggg
     2401 aggtcgtggt gccgggtggt ccgccccgga aaggcaggcc gatatactca ccgaggtgac
     2461 cggcacccaa ggccatggcg atcacgctgg gccatacccg cttgcgccgg aggatcagcc
     2521 agatgaggat gaagacgccg ttcaacagca tcagcactgg ccaggcgata ccgaaaaagg
     2581 ccaacggcca gaacgtgacc gggctcacgt gcgggctcag ttgggagagg agcacgccga
     2641 tcaccgccag gccgttcagc caccacagcg gcagcagcag gcggctcgga cgcctggccc
     2701 cgctgccggg accacccgct ggcgaggcgc cgcgttccct gggatcggtc actggataac
     2761 gggataaaat atgctcagtc cttgctcgcg tggaagagga agtcgcgctc gtccttgctc
     2821 aagctgtcgt accccgagcg ggagatcttg tccaggatgg catccacccg ggcttgtttg
     2881 tcgtgcttgg cggcgttgaa gtcggcatcc gccaggacgc cccgtcgtac cggtcgcttt
     2941 tccacccgca gccgcgatga cccccgcgcg cgaagcatgc cgagcagacc ttcgagccat
     3001 tggatgaggg acttgttcca gtcgcccttc ccgcttttga gcgccatcat ggccaggtat
     3061 ccgtaggccg cgccaccgat atgggccagg tgccctcccg tgttgctgcc ctgccggatg
     3121 ctgatcaggt cgagcaccag caccaccagg gccacccact tcagtcgcac accgccgatg
     3181 aacatgagac ttacctccat ttcgggatga taggtggcga tcccgatgaa taccgccatc
     3241 accgccgccg aggcccccag gagcatgctc gtgcccccga gtcccagatg cgcgggcagt
     3301 acggatgcca gcgcgtagaa cgcgaatccc gagagaccgc ccagcaggta catgccggtc
     3361 aaccgtttcc cacccagcag gtcctcgaac agtcgaccac tgaaccagaa catcaccatg
     3421 ttccagaaca ggtggaaggg atcgaagtgc gtgaacatgt acgtgagcac cgtccagggg
     3481 cgtctcagga gcacgggcca gttgttcgtg gccatcagcc actccagcac gcgctcatcg
     3541 tacgcgccac cattggccgc ccatgcgatg agcccgatga ggtgtacacc gaggaatacg
     3601 cccaggttga ggaagagcag gcgtttcatg gcacctcccg tgcgccactg tgccctgata
     3661 tcgtccgtga tcgccatcgt tcaacgccag ggcaggtcct gttgatgtcc cgcattcccc
     3721 cgatcgcgcc gctcgaacca tttgagcagc acgaagcccg ccaccatgcc gcccaggtgc
     3781 gcgaagtggg ccacgttgtc gcccgagcgg ttggccaccc cgctcaccag ttcgatcacc
     3841 gcgtaaatga ccacgaaata ctttgctttg atgggtacgg ggaagaagag catcatcagg
     3901 cgcacatccg ggaaaagcag gccgaaggcg agcagcactc cgaagatcgc cccgctggca
     3961 cccaccatcg gctcactcca caagcgcagg atggccgcca tgtccggtgt gatcgccccg
     4021 gtgatcgtgc cgccttggga ccactggatg aactggtcgc gcagcaaggc aagctcatcg
     4081 ggacccagcg tggcgaaatg ctccctcgat ccgtaccaca gccagcccag gtacaacaac
     4141 gccgaaccga ggccgcacag caggtagtac gccaggaagc gctgggatcc ccagcggtat
     4201 tccacctgcg gcccgaacat gtacagaccg aacatgttca gcaggatgtg caggtaccaa
     4261 ccgtcgcccg gtaccatggg gcccgcatgc acgaacatgt gcgtgaggaa ctgatacgga
     4321 cggaacagcg gcgaggacgg gtagtgcagg ccgaacagat cgtcaaattc gggtatcgaa
     4381 agcccggcga tcccggtgag cttcaccagc atgaccaggc cgttcaggat gagcaggttc
     4441 ttcaccacgg tggggatgcc accgaacacg ccgccagaag ggttgtagcg catcatcgat
     4501 cgaagcgttc gtcgagttcc tggagcgacc aggtgatgag cacaggcttg ccgcccggcg
     4561 tggtgtacgg catgccgcat gcgaagagcc ggtcgatcag gtcgtgcatc tccgttgcgc
     4621 tccattggcg cccctcgttg cgcaccatgc tccgcgccat gccccgcgca atggtcgcgt
     4681 gccgttcgga ccgcaacgcc ccgctctcgt tcttcacctg ctccagcaat tgttctacca
     4741 gttgttgcgg atcgccatcg ccgctctcgg ccggcagccc gttcaccgcg aagctgcggc
     4801 cgctgagcgg ctccagccgg aaaccgagcg cccccagttc gggcaggatc cccgtgagca
     4861 acgcgtggtc cgccgcattc agcccgatgc tcaccggaaa aagctgcgcc tggctcggtc
     4921 cctggccttt ctccagggaa cgcatcgaac gctcgtacac gatgcgctcc tgcgcgcgcc
     4981 gctgatcgat caccacgaat ccggagcgaa gttgtgcgag gatgtagcgc ccgtgcagtt
     5041 ggaacaccgg acgtgccccg aggtccatcg tttcccgcat gggcagcacg tgctgtccgc
     5101 ccacggtgtc cgtgggtgat gcttccggat ccgcccccga ccgctgcggg gcatcgtcgg
     5161 gcagatcgaa caaacgctgc caaccagcgg gtatcgtttg tcggggcagg tcccgggggc
     5221 gccagttcgc accggcgttc ccggtggcgg gaaccggggc cgtgtcaacg ccgctcatgg
     5281 cgaggagcgc gggctccggc tcgaagtcga ggctgggcgt gatgctgtgc cgccccaggg
     5341 cccgccgtgc cgcggcatgc aggatggcgt acaccgcacg gtcgtcccgg aacttgatct
     5401 ccgttttggt ggggtggatg ttgatatcga tgaaggccgg gtcgatgtgc aacaacagga
     5461 accagcccgg gtgatggtcg cgctgtacca gctcatcgta cgcacggcga acagcgtgct
     5521 ccaggtaacc gctgcggatg tagcgcccgt tcacgaaaaa atactgctcg ccccgggtgc
     5581 gccgcgcgaa ctccggcttg cagatgaagc cctccacgcc cacgatcccg gtggcctcgc
     5641 tcacgggtac cagccgctcg tcgtacttgc ggccgaacag gtgcgtgatg cgctgtcgca
     5701 ggttggccac cggcagccga tgcacctcct ggtcgttgtg gaacaggtcg aaggcgatgt
     5761 ccggatgcgc cagcgccacg cgatggaatt cctcgatggc gtgcttcaat tcgatcgggt
     5821 cgctcttgag gaactggcga cgggccggca cgttgtagaa caggctgcgt acgctgagga
     5881 ccgaacccgt cgcacaggcc accgtttcct gcgacttcac acggctgccc tcgatcagga
     5941 tccgcgtacc caactcgtcc tcgggccgcc gggtcttcaa ttccacctgt gctaccgccg
     6001 cgatgctcgc caaggcttca ccccggaagc ctttggtgcg cacggcactc aggtcatcgg
     6061 cactccggat cttgctggtg gcgtgccgtt cgaagctgag ccgtgcgtcc gtgggcgaca
     6121 tgccatggcc gttgtccgtg acctggatca gcgtgcgccc ggcgtctttc agcacaaggg
     6181 tgatgtccgt ggcgcccgca tccacgctgt tctccagcag ttcctttacc gcgctcgcgg
     6241 gccgttgcac cacttcgccc gcggcgatct ggttggccac atggtcgggc agcaggcgga
     6301 tgatgtcgga catgtcggag cgcaaatatc gcagcgcgga gggcctgtcg gtggatccgg
     6361 atcacatgca cggtcctgtc cggcggcatg caacgaaaaa ccccgatcct gagcggcact
     6421 caggaccggg gccatccggc gcgatggccg cgatcaggct tttttcttgg cggccttctt
     6481 gccacgcgtg gccttggccg taacacccgc ggccttgcgc ttggcgagtt tcaaggtggc
     6541 tttgcgtttg gcgttcaagc gggcgttgct gtcctggggc atggtcggtc ggtttgtggt
     6601 gttcccggta tcatcccgga aagcggcggc gaatgtaggc ggtggcgttc atcccggata
     6661 tggatgcggc cgccatgtcc tgaatgatgg ttcatcgtgg gcggtcgggc accacgttgt
     6721 cctcgaatcg cgagccccca ccgggcgccg gtgttgcgcc atcgcttttt ttatcgcccc
     6781 tcccgagcgg ccacaggtgc ttgcccgtga gatccggaca agggatccct ttacggcgcg
     6841 ttgccgaggt ctcacagcgc atccgccact gcacggcgag ttcgatcgtg aagggctgta
     6901 ccactccttg gggtgcgccc tggtggttgt acggcagatc ggtcagggtg cgggccgtgg
     6961 tgatgtcggt actcaggcca agggccatcc gaccggtttc gtaacctgcc agtacggtga
     7021 tcgcgttgcc gaaccggtat cccgcgccaa gaacggctgg cccccggttg atccgcaagg
     7081 ccaagttccg ctcccagtac cccgcctgcc acgacacatg gacctccgga ctgagcgtga
     7141 ggacgggcca cctgctgttt ttcccg
//
LOCUS       DAOQOC010000010         7008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4109_length_7008_cov_3.786567, whole genome shotgun sequence.
ACCESSION   DAOQOC010000010 DAOQOC010000000
VERSION     DAOQOC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_7008_cov_3.786567"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PL106_00315"
     CDS             <1..547
                     /locus_tag="PL106_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA31535.1"
                     /translation="IHYAGWCDKYQALFSSVNPVNTSHFNFSVLEPTGVVGIMSGGSN
                     GLLELVSLIAPVIAGGNSCVVVTSEKHPLPAVTFTEVLATSDLPGGVVNLLTGFRSEL
                     LKPLVSHMDINAVVCAEPTKEERQMLDTEAATNMKRVVVPKVKDWSTAEGQSPYLILD
                     TQEVKTTWHPIERGQGGGGGY"
     gene            571..744
                     /locus_tag="PL106_00320"
     CDS             571..744
                     /locus_tag="PL106_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31536.1"
                     /translation="MTTIVKRTTTRKELTALLKGKQRRRRKKGVDVKSFAGKLKLEGD
                     PLEIQKRMRDGWK"
     gene            731..1105
                     /locus_tag="PL106_00325"
     CDS             731..1105
                     /locus_tag="PL106_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA31537.1"
                     /translation="MDGSRLLVDTNIILYALKGDNALTDLIDGQELFISFVTRIELLS
                     FPKIDAAGIELIERFLQQVPVVESSPVINADAIYLRRKSGLEVPDAIIAASARFLGVR
                     LMTADKDFKKVEEELDLLLYTK"
     gene            1206..1736
                     /locus_tag="PL106_00330"
     CDS             1206..1736
                     /locus_tag="PL106_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31538.1"
                     /translation="MRFFKWLLLGTITLLVGGWVCRGPLFRSIVRYHVVGERAAIKEY
                     GAPGSVAAKVDGIIQAALDTTADRLHFSTGRVTSDPRELVPGSAANCIGYAALFAALV
                     EEDLVRAGLADRYAVEQVVSMLHIGSWNVHSLFDGPFWKDHDIVRITDKATGTMILVD
                     PVLYDVAGIDRVQGGP"
     gene            1782..2630
                     /locus_tag="PL106_00335"
     CDS             1782..2630
                     /locus_tag="PL106_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNA31539.1"
                     /translation="MGTNERTGSRPVPLPAYFGASTKADVFFHPYTNGGRPIRTQVML
                     THHLLSLVQEGEKEVIVAGHRELVDASRMVLLASTSSIMSEHDLRGRPMRSLLIFIAP
                     QFLLDFCARHGLKPKGTAAELLPMPQDDFTRLFARSVEVLGPSALNGDPALRRTKAEE
                     LLLYLHAKHPADFARFIAAAVKGRDELPLRSVVALHQDNNLSVPELAFLCHMSVSTFK
                     RRFQEAYGLAPGRYLHVRRMERAKAMLVRKLRPSEIYIELGYGSLAAFSTEFKKHFGV
                     APTAQP"
     gene            2721..3266
                     /locus_tag="PL106_00340"
                     /pseudo
     CDS             2721..3266
                     /locus_tag="PL106_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
     gene            3884..4219
                     /locus_tag="PL106_00345"
     CDS             3884..4219
                     /locus_tag="PL106_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNA31540.1"
                     /translation="MDVISWSDFEKVHLCVGTVLEIADLPNARKPAYVLTIDFGPHGI
                     KRSSAQITKHYTKDELVGRQVVAVINFPPKQVGRVMSECLVTGFPDADGDIVLTSVER
                     PLPNGARLA"
     gene            4380..5582
                     /locus_tag="PL106_00350"
     CDS             4380..5582
                     /locus_tag="PL106_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNA31541.1"
                     /translation="MRRRRHLWYSLFLVPWFVPSTTDAQVADHLLCGTRPIKEGKYQP
                     HGSVKYGDRDTSAYIIPMVFHILHQGGPENISDIQVLDGVQVLNDAFNMQAGDTLGIF
                     PGDTLVTADMEVVFCLATIDPLGMPTTGIVWYETPLTWTGMDPAAKLDPWPRDRYMNI
                     WVVAHADTVTGYTLLPEVADTLPLQDGVVILHSCLGRIGTGSEFTARALPHEAGHFLG
                     LLHPWDIPTGFGDCGDDLVDDTPITIPDIMCGSTVNCAPPTVEYLPNYMNVSYCQEMF
                     TEGQKARAHGTLNSPVAQRDQLWQASNVLQVGACGMVAVDEYKKIPDLRIYPDPFNEV
                     VVVEGCTPGPVSVVALSMDGRPEWAMNVRSGGGPLQLAPGAAVLPGAHVLRIADARGT
                     RSVLLLRE"
     gene            complement(5587..>7008)
                     /locus_tag="PL106_00355"
     CDS             complement(5587..>7008)
                     /locus_tag="PL106_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31542.1"
                     /translation="AIDPASGWMSLTPTMIGSFAVGVRVKEFRDGVQLSEVIRDFRFD
                     VVPCQAAVTSAITPQAALNGNTGLCDGLTVDMENQSAGADLYHWDFGVPGTEDDTSAL
                     DEPSFTYPAAGTYDVTLIANPGWPCADTSNAIFNVAPPVTVAFVPPPITCVDAQPLTL
                     TATGPFSNNATVTWALGSGTAPDTDAHVTHPTFPGVGTFGVQVTATDFGCTGTFTDSV
                     TLHPRPVPMFSTDTAGCIPLDAQFVNESTAWTPFTSSWSFGDGATSGENEPLHTYTTA
                     GFHTVSLTVSTSTGCIATETLVRPDLIQVWPQPIARFSVLPLVTDLMAPTVDVLDLSS
                     SAYDVTFEVDGQVFDSTSFSYTFQDAGWYTVSLMATSGLGCTDTTSTDVFVGGHFFFA
                     PNAFTPNGDGMNDTWKPEVKGAHLYRLDIVDRWGRTVFSSTDPKEAWKADGFPDGTYA
                     YKAWLSEWGPLEKEYNGRITLLR"
BASE COUNT         1377 a         2074 c         2264 g         1293 t
ORIGIN      
        1 gatccactac gccggctggt gcgacaagta tcaggcgctc ttcagcagcg tgaacccggt
       61 gaacacctcg cacttcaact tcagcgtgct ggagcccacc ggcgtagtgg gcatcatgag
      121 cggcggaagc aatggcctgc tggaactggt gagcctgatc gccccggtga tcgcgggcgg
      181 caacagctgc gtggtggtga ccagcgagaa gcatccgttg cccgcagtga ccttcaccga
      241 ggtgcttgcc accagcgacc tgcctggcgg cgtggtgaat ctgctcaccg gtttccgcag
      301 cgaactgctc aagccgcttg tgtcgcacat ggacatcaat gccgtggtgt gcgccgaacc
      361 cacgaaggag gagcggcaga tgctggacac cgaggccgcc acgaacatga agcgcgtggt
      421 ggtgcccaag gtgaaggact ggtccacggc ggagggccaa agcccgtacc tgatcctgga
      481 cacccaggag gtgaagacca cctggcaccc gatcgaacgc gggcagggtg ggggcggggg
      541 gtattgaacg ttatctttaa gatacacgcc atgaccacga tcgtcaaacg aacgactacc
      601 cgaaaggagc tgaccgccct tctgaaaggg aagcagcgaa ggcgcaggaa gaagggtgtg
      661 gatgtgaaaa gctttgcggg caagctcaag ttggagggcg acccgctgga gatccagaag
      721 cgaatgcgtg atggatggaa gtaggctgtt ggtcgatacc aacatcattc tctatgcgct
      781 gaaaggcgat aatgcgctga ccgatcttat tgatggacaa gaacttttca tctccttcgt
      841 aacacgtatc gagttgctga gtttcccgaa gatcgatgcg gccggtatcg aacttatcga
      901 gcgtttcctg caacaggttc cagtggtcga gagcagtccg gtgatcaacg cggatgcgat
      961 ctacttgcga cgcaagtcgg gactcgaagt gcccgatgcc atcattgcgg ccagtgcgcg
     1021 tttccttggc gttcgattga tgaccgccga caaggatttc aagaaggtcg aagaggagtt
     1081 ggatcttttg ctctacacga agtgaaccca tgtcttcgat ccaccgccaa cagcccccgg
     1141 aagtgaagac cacctggcac ccgatcgaac gcgggcaggg cgggggtgga tggtactgac
     1201 ggaccatgcg gttcttcaag tggctactgc ttggtacgat cacattgctg gtcgggggat
     1261 gggtgtgccg aggaccgttg ttccgaagta tcgttcgcta tcacgtggtt ggtgagcgag
     1321 cagccatcaa ggagtacggc gcaccaggat cggtggccgc gaaggtggat ggcatcatcc
     1381 aagcggcact ggacaccacg gcggaccgcc ttcatttcag cacgggccga gtgacgagcg
     1441 atccacggga gctcgtccct ggaagtgcgg cgaattgcat cggctatgcg gcactgttcg
     1501 ccgcacttgt tgaagaagac ctggtacgcg ccgggctggc ggatcggtat gcagtggagc
     1561 aggtggtcag tatgctccac atcggctcat ggaacgtgca ttccctgttc gatggcccgt
     1621 tctggaagga tcacgacatc gtacgcatca cggacaaagc gaccggcacc atgatcctgg
     1681 tcgatcccgt cctgtatgat gttgccggga tcgaccgcgt gcaaggcggg ccgtgacctc
     1741 ggaggcgacg gtccagccct tcgcatcgcg acctttgttg catggggacg aatgaacgca
     1801 ccggatccag accggtcccg ctgcccgcgt atttcggcgc cagcaccaag gcggatgtat
     1861 tcttccatcc gtacacgaac ggtggcaggc ccatccgcac gcaggtgatg ctcacgcatc
     1921 acttgctgag cctcgtacag gaaggagaga aagaggtgat cgtggccggc caccgcgaac
     1981 tggtggatgc atcacggatg gtgctgctcg catccaccag cagcatcatg agcgagcacg
     2041 acctgcgcgg ccgccccatg cgcagcctgc tcatcttcat cgcaccgcaa ttcctgctcg
     2101 acttctgcgc gcggcacggg ttgaaaccca agggcaccgc agccgaactg ttgcccatgc
     2161 cacaggacga cttcacgcgg ctcttcgcaa gatccgttga ggtcctgggg ccctccgcgt
     2221 tgaacggcga tccggcgctg cgccgtacga aagccgaaga gttgctgctc tacctgcatg
     2281 cgaagcatcc agcggacttc gcacgcttca tcgccgctgc tgtgaagggt cgcgatgagt
     2341 tgccgctgcg atcggtggtg gccttgcacc aggacaacaa cctctcggtg ccggaactgg
     2401 ccttcctctg ccacatgagc gtgtccacct tcaagcggcg cttccaagag gcgtatggcc
     2461 ttgcgccagg gcgatacctg cacgtgcggc gcatggagcg ggcaaaggcg atgcttgtgc
     2521 gcaaactgcg gcccagcgag atctacatcg agctggggta cggtagtctg gccgcgttca
     2581 gcactgaatt caagaagcac ttcggggtgg cgcctaccgc acagccctga acattggacc
     2641 gatctcgcaa ccattcgacc tgttcgcgca agccgcatgg aacagtcgag tggaactttg
     2701 ccgcaccaag atcggacctc atggataccg cctacctcag cagcgcccgc aagcagttcg
     2761 catactacaa actgctcgga gagcgcacat tcgcccagct cagcgacgag gagctgcggt
     2821 ggcaacacaa cgccgacacc aacagcgtgg ccaccatcgt gaagcacctc tggggcaaca
     2881 tgcgatcgcg gtggacggac ttcctgactt ccgatggaga gaagtcctgg cgggaccgcg
     2941 aggccgagtt cgacaacgat gtgcccacac gcgaggcgat gctggccaag tgggaacaag
     3001 gctgggcctg cttgttcgca gcgctcgata gcatcaccga cgttgacctg gaccgcatcg
     3061 tccacatccg caacgaaggc cacaccgtgc tggaggccat caaccggcag cttgcgcatt
     3121 acccgtacca cgtgggtcag atcgtacaca tcggcaagac cttgcgcggt gccgcatggc
     3181 aaagcctttc cattccgcgt ggcgggtcgg ccacattcaa cgtggaccgg ttcaacaagc
     3241 cgaagcatcg gggccatttc accgacgggg tgctgggcca cgcacagacg ataccgcttc
     3301 ttcgggagga gctgatcgaa gcgcatgagc tgctctggag tacggtgcgt gccttgggac
     3361 cgatcgacca ggaacgcgcg gaaccgggca agtggagcac cctgcagcac atggtacaca
     3421 tccatttagg ggtgaaggcc atggcaggct atctggccat gccaaaaccg gtcatcgagg
     3481 agaaattcgg ccgcctggac cgcccgagca tgagcatgga agcccttaca gagaagtact
     3541 gcacgcggtt ggcgcagggt gtggtgccac cggaccgctt cgtaccaccc gcagtgaagg
     3601 cgaaagccct gatggatctg ttcggtgaag ggcgcggtgc gctcgctgcc atgtgcgaag
     3661 cgctcctggc ctggaccgaa ccggagctgg atctgtacat gtgtccgcac ccggccatgg
     3721 gcccgctcac agctcgggaa atggtgatgt tcacggtgct gcacgcccaa catcatacgc
     3781 ggtccatcga gcggataact ggccgcgcat aggtgctcac tggccgggcc ggtgtggatc
     3841 gggaacggcc gttgcacatg gcgaagttgt cttcctttgg ggcatggacg tcatctcctg
     3901 gtccgacttc gagaaggtac acctctgtgt gggcacggtg ctggagatcg ccgatctgcc
     3961 caacgcgcgc aaaccggcat acgtactcac catcgacttc ggcccgcatg ggatcaaacg
     4021 cagcagtgcg cagatcacca agcactacac caaggatgaa ctcgtgggac gacaggtggt
     4081 ggctgtgatc aacttcccgc ccaaacaggt gggcagggtg atgagcgagt gcctggtgac
     4141 gggctttccg gatgcggacg gcgatatcgt gctcacgagt gtggagcggc cgttgccgaa
     4201 cggggcgcgg ttggcttgag tgatggcgct tagcgcggcg accggccccg taccgatcgc
     4261 aggggaagat gcccaccacc acatcgggtc gaggagccgt acctccgtgg gatactgccg
     4321 tacctcgttc cgcagcgact tttctgtgga gcggtctacc ttcgatcagc catatcgtca
     4381 tgcgccggcg acgccacctc tggtactccc ttttcctggt cccgtggttc gttccatcca
     4441 ccacggatgc ccaggttgcc gatcacctgc tgtgcggcac acgtccgatc aaggagggca
     4501 agtaccaacc acatggtagc gtaaagtacg gtgatcggga taccagtgcg tacatcatcc
     4561 cgatggtctt ccacatcctg caccagggcg ggccggagaa catcagcgat atccaggtgc
     4621 tcgatggggt gcaggtgctg aatgacgcgt tcaacatgca ggcgggggat acgctcggga
     4681 tctttccggg agatacgctg gtcacggccg atatggaggt ggtcttctgc ttggccacca
     4741 tcgatccact gggcatgccc accaccggca tcgtatggta cgagacccca ttgacctgga
     4801 ccggcatgga cccggccgcg aagttggatc cctggccgcg cgatcggtac atgaacatct
     4861 gggtggtggc ccatgccgac acggtgacgg gatacaccct gcttccggag gtggcggata
     4921 ccctgccgct gcaggatggc gtggtcatcc tccatagctg cctgggtcgg atcgggaccg
     4981 gcagcgagtt caccgcgcgc gccttgccgc acgaggccgg gcatttcctg ggcttgttgc
     5041 atccatggga tattcccacg ggcttcgggg actgtggtga cgacctggtg gacgacaccc
     5101 cgataacgat accggatatc atgtgtggaa gtaccgtgaa ttgcgcacca ccaacggtgg
     5161 aatacctgcc caattatatg aatgtgagct actgtcagga gatgttcacc gaaggccaga
     5221 aagcccgcgc gcacggtacg ctcaacagcc cggtcgcaca gcgcgatcag ctttggcagg
     5281 cctcgaatgt gctccaggtg ggcgcgtgcg gcatggtggc ggtggacgag tataaaaaga
     5341 taccggacct ccggatatac ccggatccct tcaatgaagt ggtcgtggtg gaaggatgta
     5401 cgcccgggcc cgtatcggtc gttgcgcttt ccatggacgg acgcccggag tgggccatga
     5461 acgtgcgctc gggtggtggc ccgcttcaac tggctccggg cgcggccgtc ctgccgggtg
     5521 ctcacgtgct ccggatcgcg gatgcgcggg gaacgcggtc ggtgctcctg ctccgcgaat
     5581 gagggctcag cgcaggaggg tgatacgccc gttgtactcc ttttccaagg ggccccattc
     5641 gctcagccac gccttgtacg cgtaggtgcc gtccgggaag ccatcggcct tccacgcttc
     5701 cttcgggtcg gtcgagctga acaccgtgcg cccccatcgg tccacgatgt ccagcctgta
     5761 caggtgggct cctttgacct ccggtttcca agtatcattc atgccgtcgc cgttcggggt
     5821 gaacgcgttc ggggcgaaga agaagtggcc gccaacgaac acatcggtgc tcgtggtgtc
     5881 ggtgcagccc agtccgctgg tggccatcag cgagaccgtg taccagccgg cgtcctggaa
     5941 tgtgtagctg aacgaggtgc tgtcgaacac ctggccgtcc acctcgaagg tgacatcgta
     6001 tgccgaagag gagaggtcga gcacgtccac ggtgggcgcc atcaggtcgg tcaccagggg
     6061 cagcacactg aaccgggcaa tgggctgggg ccatacctgg atgaggtcgg gccgcacgag
     6121 cgtctccgtg gcgatgcagc ccgtactggt gctcaccgtg agcgatacgg tatggaaacc
     6181 tgcggttgta taggtatgta atggttcatt ctccccgctt gtggcgccat caccgaagct
     6241 ccagctggat gtgaagggtg tccacgccgt gctttcgttc acgaactggg catcgagcgg
     6301 aatgcagcca gccgtatccg tggagaacat cggcacgggc cgggggtgca gggtcacgct
     6361 gtcagtaaag gtacctgtgc atccgaagtc cgtggcggtg acctgcacgc cgaatgtgcc
     6421 gaccccgggg aaggtcggat gcgtcacgtg ggcatcggtg tccggcgcgg tgccactgcc
     6481 gagggcccag gtcaccgtgg cgttgttgga gaacggaccg gtagccgtga gcgtaagggg
     6541 ctgggcatcc acgcacgtga tcggtggagg aacgaaggcg accgtgaccg gtggcgccac
     6601 gttgaagatc gcgttgctgg tgtcggcgca gggccagccg ggattggcga tcagcgtgac
     6661 atcgtaggta ccggcggcag ggtaggtgaa cgatggctcg tccaacgcgc tcgtatcgtc
     6721 ctcggtaccg ggcacgccga agtcccagtg atacaggtcc gcacccgcgc tctggttctc
     6781 catgtccacc gtgagcccgt cgcacaaacc cgtgttgccg ttcagagctg cttgcggggt
     6841 gatcgccgag gtgaccgcgg cctggcaggg gaccacatcg aagcggaaat cccggatcac
     6901 ctcgctcaac tgtacaccat cgcggaattc cttcacgcgt acgcccacgg cgaaggaccc
     6961 gatcatggtg ggggtaaggg acatccatcc gctggccgga tcgatcgc
//
LOCUS       DAOQOC010000011         7002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4112_length_7002_cov_3.732978, whole genome shotgun sequence.
ACCESSION   DAOQOC010000011 DAOQOC010000000
VERSION     DAOQOC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_7002_cov_3.732978"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PL106_00360"
     CDS             <1..696
                     /locus_tag="PL106_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31543.1"
                     /translation="PGALSEGSTVSGQLHLWSGFSTEQECMWPVTWDLCPEQACMTVA
                     PYLSNLGDGIITATVPYTLEDSEGLILGNGTFVINPNNQGVIGQELCLPPGSYFLQLE
                     QVGVVGGQLFFGLMTPALNSEMTQTAYVQGTGVNTAAITILERCSDLPNTLPDERTDD
                     HRFHLTMSGDELIITGTDHLPLGRLELLDAMGRSIGNTFTTSTRHTWDLSGLSDGVYL
                     VRGSRYRAVRFVH"
     gene            complement(926..1252)
                     /locus_tag="PL106_00365"
     CDS             complement(926..1252)
                     /locus_tag="PL106_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31544.1"
                     /translation="MGSQQKSIEEARTFIRLAFVACTGLAFYYAHLFLGLFQNVFLFR
                     TLAIVFMLFALPLPIIAFNNKKLFPEVKGNGKVMLNWATMLLFAHHFLMTFIFVMILQ
                     GEGRVF"
     gene            complement(1295..2020)
                     /locus_tag="PL106_00370"
     CDS             complement(1295..2020)
                     /locus_tag="PL106_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31545.1"
                     /translation="MRTHATWIRLVPWMTAWAVAMAFLESAVVIYLRELYYPDGFKFP
                     LAPVAPHVAVTEVLRELATMVMLLAPAALVTRKRLERFAWFSFCFGVWDIFYYVFLKI
                     ILDWPASWLEPDILFLVPVAWSGPVLAPCIISVGLISLGCTLLWRRTRFPGYRPRTGH
                     WVSLGASAVLFLYTFLEEPVSYIRGAGGTLETMVGHRAMDALGAYVPEHYAWWVFLAA
                     CVLGGATLGMMLLGQGRATVGDP"
     gene            complement(2017..2952)
                     /locus_tag="PL106_00375"
     CDS             complement(2017..2952)
                     /locus_tag="PL106_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNA31546.1"
                     /translation="MSRRIDVDFTDGPVVAGVLLAGACTITFAPDPFVLMIVRAFQWQ
                     WVLVAAGLACAALLSRWWWTAAAAGAGTLLMAFPVCTPTDPQTVGAMSRDLVHVAQMN
                     VLQPNDDHAAVIVAARSTGADVISFQEVSPAWGRALSQGLAEDYPFHHIVPGSNCYGI
                     ALFSRLPFERITTVDLGGHPMLLAEVRTPCGPVRLLCVHATSPGSWKQFRERNAQLDE
                     LAALVNDSPVPVVLFGDLNTVSWDHAFRRLCARTGLREHGGDRRATWPSMAGIALIPL
                     DHVLVSRELAVGEFSIFSIPGSDHRGVAAVIRSRS"
     gene            complement(2949..3431)
                     /locus_tag="PL106_00380"
     CDS             complement(2949..3431)
                     /locus_tag="PL106_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31547.1"
                     /translation="MDPARIHRRIRTWTILFIAALWSSGLTAVPLEAGTRWLSATTTA
                     WPGPWHGWAEHTALAIADVNAHHPFLFYGTDWLAFAHIVIGIAFFGVLRDPVRNRWIV
                     EWGLWCCLLVPVLAFAWAPVRGIPFFWRCVDASFGVIGAVPLLIVLRDIGTLEKPVRP
                     "
     gene            complement(3434..4972)
                     /locus_tag="PL106_00385"
     CDS             complement(3434..4972)
                     /locus_tag="PL106_00385"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNA31548.1"
                     /translation="MRSTRFALVTVLLAAVIFDRLFWHQSIGVNLTLFALIILGILLA
                     DHGWRGWGPPARWTAAGTLVSVAMVAVHGSTIATIAAFASLFTCTAFVLSPRLRSTPM
                     ALLGWVVNLVASPIGINSNVAEVMPGTPVLRRSWWWGRVILLPLLVLAVFFTLYSGGN
                     SRFHAMTAGFLSDLSAFLEDLLEKAFTPHMLFFLFAVVFTSSLLMRSIGEWLSDHESR
                     FGDMMRRVRIKRPQWLPPLDMAALDRERRMALVLLVLVNSLLLVVNAIDVHWIWFGFR
                     VEPGMSLKEFVHEGTWLLIVSILLSMAILLRLFRGNLNFHPENRWLKVLATVWIVQNS
                     ILGISVFLRNYHYIGFHGLAYKRIGVIVFLALVLVGLFTLYLKVRQRRTLFHLLRVNA
                     WAAFVMLVGLGTVDWDSLIVRYNLAHWNQGEIDVDNYLAMSDKVLPILFAHSDRVYEQ
                     MRKHQENEVRWVTQLDPQAFAEQLEQRRVRFLRRYLEQGWPSWTWADARTNNALATVS
                     NSAQ"
     gene            complement(5032..5328)
                     /locus_tag="PL106_00390"
     CDS             complement(5032..5328)
                     /locus_tag="PL106_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNA31549.1"
                     /translation="MIGGLHKAFESRVRLGIMAVLTVNDHVEHGTLRDLLEVTDGNLA
                     SHIAALVEVKYVQVRKRFVGKRPNTSYKATAAGTRAFTEHLGAMEDLLKRDLRR"
     gene            complement(5325..5960)
                     /locus_tag="PL106_00395"
     CDS             complement(5325..5960)
                     /locus_tag="PL106_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31550.1"
                     /translation="MSGDLAHIRTLMERSSRFISLSGLSGVFAGVMALGGARLAQYHV
                     ARAIGSPDDGVTYDLARTGVFQLDELSITLLVDAIMVLVLALLGAFWFTWRRSRHTGQ
                     RLWDPSARRLAWNLLIPLAAGGLTCLALLYHGLPGLVAPATLVFYGLALLNASKYTLD
                     EIRWLALSELALGVVACFWVGAGLLFWALGFGVLHIVYGGLMWMRHERGKA"
     gene            complement(6123..6830)
                     /locus_tag="PL106_00400"
     CDS             complement(6123..6830)
                     /locus_tag="PL106_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNA31551.1"
                     /translation="MGPLPLILLFIGLPLLAGLWLRRREAESGLVKLLLAFSGAFLLS
                     VTVVHMLPELYEQGGHGIGPWVLAGFMLQVVLEFFSRGIEHGHVHVHAGHARTLPLMT
                     LFSLCAHSFTEGLPFADEAVRANLPFVVGVLMHKAPMAIALATVLRRSDVTDGRYWTM
                     IGVFAISLPIGMLVGIAGGDRLGGGFLEAALALAIGMLLHISTTIIFETTPDHRFDPK
                     RFLTVLAGAGLAFLSMH"
BASE COUNT         1510 a         2207 c         2151 g         1134 t
ORIGIN      
        1 ccgggcgcgc tgtccgaggg ctccaccgta tcgggccaac tccatctctg gtccggtttc
       61 tctaccgagc aggaatgcat gtggccggtc acgtgggacc tgtgccctga gcaagcatgt
      121 atgaccgtgg ctccgtacct gtccaatctc ggggatggta tcatcaccgc aacggtccct
      181 tacacgttgg aagacagcga gggtctcata ctgggcaatg gaacattcgt gatcaacccg
      241 aacaaccagg gggtgatcgg ccaggaactc tgcctccctc ccggttcgta cttccttcaa
      301 ttggagcagg tgggcgtcgt tggtggtcag cttttcttcg gactgatgac ccccgccttg
      361 aactccgaaa tgacacagac cgcttatgta cagggaacag gggtgaacac cgcagcgatc
      421 accatactcg aacgctgctc ggacctgccc aacaccttgc ccgacgaacg caccgatgat
      481 cacaggttcc acctcaccat gagcggtgat gaactcatca tcacaggcac cgatcacctt
      541 ccgctcggcc gactggagct gttggacgcc atggggcggt cgatcgggaa cacgttcacc
      601 acaagcacgc gtcatacttg ggacctctcc ggtctgtccg acggtgtcta tctggtgcgt
      661 ggttcgcggt acagggctgt acgcttcgtg cattgagatg cccacacccc cgaatacgaa
      721 gcgcgcctta cggggcgcgc ttcgagtatc ctggcctccg agcttacggg ctcggacaga
      781 ggctttttat gcgcttcggc ttttattgcc tcctgtgacc agggatattt tcaggaccgg
      841 taccggggct accgtggatc ttcaacgtgg aagcaccgga gaaagtttca cgactctgcc
      901 attttccacg gatcggatca acgattcaga acacgcgtcc ctccccctgc aggatcatca
      961 cgaaaatgaa ggtcatgagg aagtggtggg caaagagcag catggtggcc cagttgagca
     1021 tcaccttacc gttgcctttc acctccggga acagcttttt gttgttgaat gcgatgatgg
     1081 gcaatggcag tgcgaaaagc atgaacacga tggccagcgt gcggaagaga aaaacgttct
     1141 ggaacagccc caggaagagg tgggcgtagt agaaggccag gccggtacac gctacgaaag
     1201 ccagtcggat gaaggtgcgg gcttcttcga tcgatttctg ctgtgatccc atcgctccgg
     1261 aatgcaaggc tctaacgacg tgatcccgcc atggttacgg atcgcccacc gttgcgcgac
     1321 cctggccgag cagcatcatc cccagcgttg ccccacccag cacgcatgcg gcaaggaata
     1381 cccaccatgc atagtgctcc ggaacatacg ctcccagcgc atccatggcc ctgtgtccga
     1441 ccatcgtctc cagcgtcccc cccgctcccc ggatgtagct gaccggttcc tccaggaagg
     1501 tgtaaaggaa caggacggca ctcgcaccca atgataccca atgtccggtc cggggtcggt
     1561 aacccgggaa cctggtccgc cgccacagga gcgtacagcc aagggagatc aggcccacgg
     1621 agatgatgca aggtgccagc accggtccgg accaggccac cggcaccagg aacaggatgt
     1681 ccggttccaa ccagctggcg ggccagtcca ggatgatctt caagaagacg tagtagaaga
     1741 tgtcccacac accgaaacag aagctgaacc aagcgaagcg ctccaaccgc ttccgggtga
     1801 ccagggccgc aggggccagc agcatcacca tggtggccag ttcgcgaagc acctcggtaa
     1861 cggccacatg cggcgcaacg ggcgccagcg ggaatttgaa accgtccggg taatacaact
     1921 cgcggaggta gatgaccacg gcgctctcca ggaaggccat ggcgacggcc catgccgtca
     1981 tccagggtac gagccggatc catgtggcat gcgtcctcat gagcgcgatc ggatgacggc
     2041 cgccacaccg cggtggtcgg atccggggat ggagaagata ctgaactcac ccacggcaag
     2101 ttcccggctc accagcacat ggtccagtgg gatcagcgcg atccccgcca tggatggcca
     2161 tgtggcgcgc cgatctcccc cgtgctcgcg caagcccgtg cgcgcgcaca acctcctgaa
     2221 cgcatggtcc cagctcaccg tgttcaggtc accgaagagg accacgggca cggggctgtc
     2281 attcacaagg gccgcgagct cgtccagttg ggcgttgcgt tcgcggaact gcttccagct
     2341 ccctggtgag gtggcatgaa cgcacaacag acggaccggg ccgcacgggg tgcgcacctc
     2401 ggccagcagc atcggatggc cgcccaggtc aacggtggtg atccgctcga acgggagtcg
     2461 gctgaacagg gcgataccat aacaattact cccgggaacg atgtggtgga acgggtagtc
     2521 ttcggcaagg ccctgcgaca gtgcccgccc ccacgcgggg ctgacctcct ggaacgagat
     2581 cacatcggca cctgtggacc gggccgcgac gatcacagcg gcatggtcat cattcggttg
     2641 cagcacgttc atctgcgcca catggacaag gtcacggctc atcgccccca cggtctgcgg
     2701 gtccgtcggg gtgcaaaccg gaaaggccat cagcaacgta ccggcaccgg cagccgcagc
     2761 cgtccaccac caccgtgata agagggccgc acacgcaaga cccgccgcca cgagcaccca
     2821 ctgccactgg aacgcacgca cgatcatcag cacgaagggg tcgggcgcga acgtgatcgt
     2881 acaagcgcca gcgagcagca caccagccac taccggacca tcggtaaagt ccacatcgat
     2941 ccggcgactc atgggcgtac gggcttctcg agtgtaccga tatcccggag cacgatcagc
     3001 aacggcaccg caccgatcac gccgaaggag gcatcaacac agcgccagaa gaaggggatc
     3061 ccgcgaaccg gcgcccaggc gaacgcgagt accggaacga ggaggcagca ccagaggccc
     3121 cactccacga tccacctgtt ccgcacggga tcgcggagta ccccgaagaa cgcgataccg
     3181 atcacgatgt gcgcgaaagc cagccagtcg gtaccgtaga acaggaatgg atgatgcgcg
     3241 ttcacgtcgg cgatcgccag cgctgtgtgt tcggcccatc catgccaggg accgggccag
     3301 gcggtcgtgg tggcactcaa ccatcgggta cccgcttcca acggaacagc ggtgaggccg
     3361 ctggaccaca gcgcggcgat gaagaggatc gtccaggtac ggatgcggcg atggatgcgg
     3421 gcggggtcca tgatcattgc gcggaattgc tcacggtcgc caacgcgttg ttggttcggg
     3481 catcggccca ggtccaactt ggccaccctt gttcaaggta gcgccgcaag aacctgaccc
     3541 tgcgctgctc gagttgctcg gcgaaggcct gcgggtccag ttgggtcacc cagcgcacct
     3601 cattctcctg atgcttgcgc atctgttcat agacccggtc cgagtgcgcg aagaggatcg
     3661 gcaacacctt gtcgctcatc gcgaggtaat tgtccacatc gatctccccc tggttccaat
     3721 gggccaggtt gtaccggacg atcaggctgt cccaatccac ggtgcccagg cccaccaaca
     3781 tgacgaaagc cgcccaggca ttcacgcgca acaggtggaa gagcgtgcgg cgctgacgca
     3841 ccttcagata cagggtgaaa agccccacca ggacgagggc caggaacacg atcaccccga
     3901 tgcgcttgta agccagtccg tggaaaccga tataatggta gttgcgcagg aaaacggaga
     3961 tgccgaggat ggaattctgg acgatccata cggtggccag caccttcaac cagcggttct
     4021 ccggatggaa gttgaggttc ccgcggaaga ggcgcagcag gatcgccatg ctcagcagga
     4081 tgctcacgat gagcagccac gtcccttcat gcacgaattc cttcaagctc atgcccggtt
     4141 ccacccggaa cccgaaccag atccagtgga cgtcgatggc gttcaccacc aggagcaggg
     4201 aattcaccag caccaggagc accagggcca tgcggcgctc acgatccaac gcggccatgt
     4261 ccaggggtgg caaccactgc gggcgtttga tccgcacgcg ccgcatcata tccccgaacc
     4321 gcgattcgtg gtcggagagc cactcaccga tggaacgcat cagcagcgag gacgtgaaca
     4381 ccacggcgaa caggaagaac agcatgtggg gcgtgaaggc cttctcgagc aggtcctcca
     4441 ggaacgcact gagatccgag aggaaaccgg ccgtcatcgc gtggaagcgg ctgttgcctc
     4501 cactgtacaa cgtgaagaac acggcgagca cgagcagcgg aagcaggatc acacgccccc
     4561 accaccagct ccgacggagc accggggtgc ccggcatgac ctcagccacg ttggaattga
     4621 taccgatcgg ggaggccacc aggttcacca cccagccgag cagggccatg ggcgtggagc
     4681 gcagacgggg cgacagcacg aaggcggtac acgtgaacag cgatgcgaac gcggcgatgg
     4741 tggcgatggt ggagccgtgt accgcgacca tcgccacgct caccagcgtg ccggcagcgg
     4801 tccaacgggc aggaggaccc catccgcgcc agccgtggtc cgccagcaat atgcccagga
     4861 tgatgagggc gaacagggtg aggttcacac cgatggactg gtgccagaac agccggtcga
     4921 aaatgaccgc ggcgagcagg acggtgacca gggcgaagcg tgttgatctc atgtttttca
     4981 gtatgttatt ggtgccgggc cgcaggacgt ggccgccggg gtagcgaagg gtcatcggcg
     5041 cagatcgcgt ttcaacagat cctccatggc tccgaggtgt tcggtgaaag ctctcgttcc
     5101 tgcggccgtg gccttgtagc tcgtattggg ccgtttgccc acgaaacgct tgcgcacctg
     5161 cacgtatttc acctccacca acgcggcgat atggctggcc aggttgccat ccgtcacctc
     5221 gagcagatcc ctgagcgtgc catgctccac atggtcgttc acggtgagca cggccatgat
     5281 gcccaaacgc acccggctct cgaacgcctt gtgcagaccg ccgatcatgc ctttccgcgt
     5341 tcgtgtcgca tccacatgag gcctccgtac acgatatgaa gcacgccgaa gccgagggcc
     5401 cagaaaagca gcccggcgcc cacccagaaa caggcgacca cgccgagggc cagttcgctc
     5461 aaggccagcc agcggatctc gtccagcgtg tacttgctgg cgttcagcag agccagtcca
     5521 taaaatacca gtgtggccgg tgccaccagt cccggcaggc cgtggtaaag gagggccaga
     5581 caggtcaggc cgcccgccgc cagggggatc aacaggttcc aggcgaggcg tcgggcgctg
     5641 ggatcccaga gccgctgtcc ggtgtggcgg ctgcggcgcc aggtgaacca gaaggcaccg
     5701 agtagtgcga ggacgagcac catgatggcg tccacaagca gggtgatgga cagttcatca
     5761 agctggaaga cgccagtccg cgccaagtca tacgtgaccc cgtcgtcggg tgatccgatc
     5821 gctcgtgcca catggtactg tgccagccgg gccccgccca gggccatcac gccggcaaaa
     5881 acgccgctga gcccgctgag cgagatgaaa cgcgaactgc gctccatgag tgtgcggatg
     5941 tgcgcgagat cgccggacaa tgcccaggtg tcgttcctgt gcccttcttg atccattaca
     6001 aagtactttg agttgcaaag ttagaacgga cccttccatg ttctggtatg tcgctcaaga
     6061 aaaatagagg accgggttga tcactggcac tgcccggatc ggatccgttc gatccgcgct
     6121 gctcagtgca tgctcaggaa tgccagtcca gcgcccgcga ggacggtcag gaaccgtttc
     6181 ggatcgaagc ggtgatccgg cgtggtctcg aaaatgatgg tggtgctgat gtgcagcaac
     6241 atgccgatcg ccagcgcgag cgcggcctcc aaaaacccgc cacccaggcg gtcccccccc
     6301 gcgatgccga ccagcatacc gataggcagc gaaatggcga agacaccgat catcgtccaa
     6361 tagcgtccgt cggtcacatc gctgcggcgc aacacggtgg ccaatgcgat ggccatgggg
     6421 gccttgtgca tgagcacgcc gaccacgaaa ggcaggttgg cgcgcacggc ttcatcggcg
     6481 aacggcagac cctccgtgaa ggaatgggca caaaggctga agagtgtcat caggggcaac
     6541 gtgcgcgcat ggcccgcgtg aacatgcacg tgcccgtgtt cgatgccccg gctgaagaat
     6601 tcaagcacca cctggagcat gaagccggcc aatacccacg ggccgatacc gtgcccgccc
     6661 tgctcgtaca attcgggcag catgtgtacc acggtcacgc tgagcaggaa ggcgccgctg
     6721 aaggcgagca acagcttcac caggcccgat tcggcctcgc gacgccgtag ccagaggccc
     6781 gccagcagtg gcagtccgat gaacaggagg atgagcggca ggggccccat ggcgaaagga
     6841 cggcgaaagt accccggaag cagggtgcat gatcacgtgt cgctgcgccc gccaacacgt
     6901 ccgcatccgg ccgcaacgta tccgcccacg cccgtgttca tggatcagtg gaaaactccg
     6961 ggggcgttct ccgcggccca cgtgccatgc acgttgtttt gc
//
LOCUS       DAOQOC010000012         6938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4158_length_6938_cov_3.840477, whole genome shotgun sequence.
ACCESSION   DAOQOC010000012 DAOQOC010000000
VERSION     DAOQOC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4158_length_6938_cov_3.840477"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1847
                     /locus_tag="PL106_00405"
     CDS             <1..1847
                     /locus_tag="PL106_00405"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNA31552.1"
                     /translation="SDVCCGFQDIAGDIHLEFKLATIDPEGNCTNGIDRIRSCETFVG
                     DNGSKLNWWPRNKYLNVWTVQTIRSGAAGYSQYPSAVEGPNAPADGTLLLHDYIGRIG
                     SGAEFKSRALTHEVGHWLNLKHVWGDNNGEDGAPQDHMSPTCGDDAVDDTPWTRGWNT
                     CPTPAQSKNCSDTIYENFQNYMEYSYCSMMFTEGQNERMRAALASGLAERDNLWTPET
                     LAATGTDGLTDQHCAPVADFYCFNDAIATGNPNTPQGTDFYCIGDQVSFFDNSQRAMP
                     DSWQWTFQDGEPSTSTDRNPTVSFNSGGWKTVSLTVSNAQGSSTRVNEFSVFISYPWS
                     VLSGAVQEDFEGSCADCYWFTNNYDADESFWHHVSNAGHSGGSSMMLNAKDSYGINDY
                     FIDEGADDIDALVTPDMDLTWLQNGEFSFWYAYAVSTTDMEEVTEKLEIWSSTNCGRN
                     WQLRKTIDEEDIITAGTTSAPFVPGSPAVWQQESFNLASSFATDHVRFKFVFYSGEKS
                     NNLYIDDVNITGTVGFNDLQAGTTGLTVMPNPTEGEVSVVYALSAPGKGRIAMLDAQG
                     REVWSRATRDLAQERVTIDTRALGLAAGVYTVRLAHETGQRVERLVVR"
     gene            complement(2088..3722)
                     /locus_tag="PL106_00410"
     CDS             complement(2088..3722)
                     /locus_tag="PL106_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA31553.1"
                     /translation="MLKSAHTEFLIAALALLLFVPGLGAVNLFDWDEINFAEIAREML
                     VTDDPLQPRIAFQPFYEKPPLFMWMQALCMKVFGVGEFAARLPNAICGAVTLLTLYRM
                     GSRLRNGAFGLLWVLAYLGSILPNLYFRSGIIDPWFNLFIFAGLHAYIRAAGGDGPRH
                     AALCGLWLGLAVLTKGPVAVLIPGITVVVYALRQRRWPMVWIRTISVAAPILLLTIGT
                     WAVADLLRNGPDFMSGFFWRQVAMLTTEDAGHGGFLGYHFVVLLLGCFPASLFAIHEM
                     TRRSTDSDRQREHRRWMIILFWTVLLLFTIVRTKIVHYSSLCWFPLTYLAALSMERLW
                     RGEARAPMWLRVSLGGIGTLVALIFMAAPFLGMHIDVIKPLVADPFGRANLDAEVAWT
                     GVEALAGIWMAVVLFLGHRYFARGDLKRGLPVVFGGTAIFVSLALFFFINNIEGYSQR
                     AAIDFFKACARSSDEGRPVYTITRDYKSYAQLFYARTLPPADPRAHNEEWLFHGPIDR
                     PVYLSAKITGEESVKAIGTFTELNRKNGFIFYRRDP"
     gene            complement(3741..3890)
                     /locus_tag="PL106_00415"
     CDS             complement(3741..3890)
                     /locus_tag="PL106_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31554.1"
                     /translation="MDRPLLYHRLSKYQLWIRPVHVSQCKLGQCDGFQSALHKLIGGK
                     QNADL"
     gene            complement(4216..5535)
                     /locus_tag="PL106_00420"
     CDS             complement(4216..5535)
                     /locus_tag="PL106_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31555.1"
                     /translation="MPHLADAQFWASAGLPGTSVVLQEVYATPNEDTLYYCGTLQLNG
                     VSWWASNSIMRLTAQGWDTLGVVHGVVTTVVHWHDTLVVGGAFTTVNSDTMYNVAAHV
                     NGNWLPFGVFQDLSVRRLRIIDEELYAVGSFDSVDGDTTIVGIAVRRGGAWHRVGNSV
                     EQDDPQLLDLTSYNGSIAAIGLMGNEDGRGIMQLIDSTWQLVGPGIQGGLSGAHSIME
                     YQGDLYVGGQLRLGEGNAGQDIMRWDGSQFHALGLGLQRELGNTYTFSDCRAMRIHDG
                     LLFVGGGFRYAGGIPSDGVAVWDGSQWCGVPGILTEAGGGAMSIAFYHDTLFVACGWL
                     ADGDSVNQAARFIGASYIDSCSGPLSTNSPLPLPEAERVFPNPATSVIRFTPPFSRNE
                     QAVIVDALGRIVVSLSGRVPEFDVSAWPRGMYTVLSTKGPATRFLLQ"
     gene            complement(5768..6544)
                     /locus_tag="PL106_00425"
     CDS             complement(5768..6544)
                     /locus_tag="PL106_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31556.1"
                     /translation="MPTAVIVDAIRTAGGKRNGDLSGWHSADLAAEVLKALVERNNLD
                     PTLVDDVIMGCVMQVGAQSLNVGRNAVLAAGFPESVPATTIDRQCGSSQQSAHFAAQG
                     VMAGAYDIAIAAGVEVMSLVPMGASFGDMKRIGFPFGEQVGLRYADQGGLVPQGISAE
                     MIADKWGLSREDLDAFGAESQRRAAQAQSEGRFDNEIVAQICWNGHEIAEITCPTKYF
                     DEASSINFSRSMTYGLGVVGTSWRYFLARTGLVKWALLEA"
     gene            6714..>6938
                     /locus_tag="PL106_00430"
     CDS             6714..>6938
                     /locus_tag="PL106_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31557.1"
                     /translation="MATSTDEQLRELTKGQTVPTEFLKTVTEYGSSRALRWMEGEDWK
                     ELTFSQLADQAARVAAGLAAGREAVGAQLLA"
BASE COUNT         1454 a         2106 c         2123 g         1255 t
ORIGIN      
        1 tgagcgatgt gtgctgtggc ttccaggaca tcgccgggga catccatctg gagttcaaac
       61 tcgcgaccat cgatcccgaa ggcaattgca ccaatggcat cgatcgcatc cgcagctgcg
      121 agacctttgt tggcgacaat ggctccaaac tgaattggtg gccgcgcaac aagtacctga
      181 acgtttggac cgtgcagacc atccgcagcg gtgcggcggg ctactcgcag taccccagcg
      241 cggtggaagg gccgaacgcc ccggcagatg gcacgttgct gctgcacgat tacatcggcc
      301 ggatcggctc tggcgcagaa ttcaagtcgc gtgcgcttac gcatgaagtg ggccactggc
      361 tcaacctgaa gcacgtctgg ggtgataaca atggtgaaga tggcgcacct caggatcata
      421 tgtccccgac ctgtggggat gatgccgtgg atgatacgcc atggacccgt ggctggaaca
      481 cctgccccac cccggcacag agcaagaact gcagcgacac catctacgag aacttccaga
      541 actacatgga gtattcctac tgctccatga tgttcaccga aggtcagaat gagcggatgc
      601 gcgcagcact ggcctcgggc cttgccgagc gcgacaacct ctggaccccc gagaccctgg
      661 ccgccactgg caccgacggg ctgaccgacc agcactgcgc tcccgtggcg gatttctatt
      721 gcttcaatga tgccatcgcc accggcaacc cgaacacccc gcagggaacg gatttctatt
      781 gcatcggtga tcaggtatct ttctttgaca acagccagcg cgccatgccc gattcctggc
      841 aatggacctt ccaggacggg gagccctcca ccagcacgga tcggaaccct accgtgagct
      901 tcaactccgg gggatggaag accgtgagcc ttacggtgag caacgcccag ggatccagta
      961 cccgcgtgaa cgaattcagc gtgttcatct cctacccgtg gtccgtgctc tccggtgcgg
     1021 tccaggagga ttttgagggg tcgtgcgcgg actgctactg gttcaccaat aactacgatg
     1081 cggatgagag tttctggcat cacgtgtcca atgccgggca cagcggaggt tcatccatga
     1141 tgctcaacgc caaggacagc tatggcatca acgactactt cattgatgaa ggcgcggatg
     1201 acattgatgc gttggtgacc cccgacatgg atctcacttg gctccagaac ggagagttct
     1261 ccttctggta cgcctacgcg gtctcgacca ccgacatgga agaggtgacc gagaaactgg
     1321 agatctggtc ctccaccaat tgcggaagga actggcagtt gcgcaaaacg atcgacgagg
     1381 aggacatcat cacggcaggg accacctcgg cacccttcgt gccgggttcg cccgccgtgt
     1441 ggcaacagga gtccttcaac ctggcatcga gcttcgccac ggaccatgtc cgattcaagt
     1501 tcgttttcta ctcgggtgag aagtccaaca acctctacat cgacgacgtc aacatcaccg
     1561 gtacggtggg cttcaacgac ctgcaggccg gtaccacggg cctcaccgtg atgcccaatc
     1621 ccaccgaggg tgaagtatcc gttgtatacg cgctctcagc gccgggcaag ggccgcatcg
     1681 ccatgctcga cgcccaagga cgcgaagtgt ggtcgcgtgc cacccgcgat ctggcgcaag
     1741 agcgtgtgac catcgatacc cgcgcgctcg gcctggccgc tggcgtgtac acggtccgcc
     1801 tggcccatga gaccggccaa cgtgtggagc gtttagtggt gcgttgaacc ggaagcggat
     1861 cattcttagc ggggcccggc atgccgggcc ccgcttcgtt tccacacgtg tgcggaggtg
     1921 accgaccgca ctatcgaata ggcctccgtc ccaattgccc ggcaatgaaa aagacgtgtc
     1981 acggtgagcc tgccgaccgc gcctttgcgt agccgctacg gcgaagcaag gaccggttcg
     2041 acgggctcag cgtgacatat cggatgaccc catactgcga aatggtatca gggatcgcgt
     2101 cggtagaaaa tgaacccgtt cttccggttc aattccgtga aggtgccgat cgccttcacg
     2161 ctttcctccc cggttatctt cgcggacagg tacacgggac gatcgatagg gccatggaag
     2221 agccattcct cgttgtgcgc acgagggtcg gctggtggca acgtacgggc gtagaagagt
     2281 tgcgcatagc tcttgtaatc gcgggtgatg gtgtacaccg ggcggccctc gtccgatgac
     2341 ctggcgcacg ctttgaagaa gtcgatggcg gcgcgctggg aatagccttc gatgttgttg
     2401 atgaagaaga agagcgccag gctcacgaag atcgcggtgc caccgaagac cacagggagg
     2461 ccccgcttca gatcgccacg agcgaagtac cggtggccca ggaagagcac cacggccatc
     2521 cagatgccgg ccagcgcctc caccccggtc caagccacct ccgcatccag gttcgcacgg
     2581 ccgaacggat cggccaccag gggcttgatc acatcgatgt gcatgcccag gaacggggcg
     2641 gccatgaaga tcaacgccac gagggtgccg ataccaccga gtgatacgcg caaccacatc
     2701 ggagcccggg cttcgccacg ccagagacgc tccatgctca gtgccgccag gtaggtgagc
     2761 gggaaccagc agaggctgct gtagtgtacg atcttggtgc gcacgatggt gaagagcagc
     2821 agcacggtcc agaagaggat gatcatccag cggcggtgct cgcgttgtcg gtcgctgtcc
     2881 gtggaacgac gggtcatttc gtgaatggcg aagagggagg cggggaaaca accgagcaac
     2941 agcaccacga aatgataccc gaggaatccg ccgtgcccgg catcctcagt ggtgagcatg
     3001 gccacctggc gccagaagaa gcccgacatg aagtcggggc cattgcgcag gaggtcggcc
     3061 acggcccatg tgccgatggt gagcaacagt atcggtgctg caacggagat ggtccggatc
     3121 cagaccatgg gccatcggcg ctgccgtagc gcgtatacga ccacggtgat gccggggatg
     3181 aggacggcca ccggaccctt ggtgagcacg gccaggccga gccagagccc gcacaacgcg
     3241 gcatggcgtg ggccatctcc gcccgccgca cggatgtacg cgtggagacc ggcgaagatg
     3301 aagaggttga accaagggtc tatgatccca ctgcggaagt acaggttggg gaggatcgaa
     3361 ccgagatagg ccagcaccca aagcaggccg aaggccccat tgcgcaaacg ggagcccatg
     3421 cggtacagcg tcagcagggt gacggcgcca cagatggcat taggcagccg cgcggcgaat
     3481 tcacccaccc cgaagacctt catgcacagc gcctgcatcc acatgaacag gggcggcttc
     3541 tcgtagaagg gctggaaggc gatacggggc tggagcgggt cgtcggtcac gagcatttcc
     3601 cgggcgatct ccgcgaagtt gatctcgtcc caatcgaaga ggttcactgc accgaggccg
     3661 ggcacgaaga ggagcagagc gagtgctgcg atgaggaact ccgtgtgcgc gctcttcaac
     3721 atgccttgtt ctggtccgcg tcaaagatcc gcattctgct ttccgccgat cagcttgtgc
     3781 agtgcgctct ggaagccatc gcattggcca agcttgcatt ggctcacatg tacgggacgg
     3841 atccagagct ggtatttact cagtcggtga taaagaagcg gtcgatccat gggttcaaaa
     3901 gtgcgagagt gcaagggttt gagcctcgcg ctctcgcact cttgcaccct tcgacccacc
     3961 atggttgatc gccgatcaaa ccggatctta accaccgact gaggaacggg cgggaatgtc
     4021 tgaggaggaa gaagctctgc ggcggggtgg agcaggccaa ggaggatccg gaacgaggag
     4081 gatttcccca agccgcaggt gccttttcgc gcggtcaaac cgaatttccg caggcccgtg
     4141 atcgatgcgc gtggcagcgg ctataggcat gggagagcgt gcccaaggta cgccacgccc
     4201 ggtgttggtt gaagatcatt ggagtaggaa ccgtgtggct ggccctttgg ttgaaagcac
     4261 ggtgtacata ccgcgtggcc atgcggacac atcgaattcc gggactcgac ccgagaggct
     4321 cacaacaatg cggcccaagg cgtccactat cacggcttgt tcattccggc tgaacggtgg
     4381 ggtaaatcga atgaccgatg tggccgggtt ggggaaaacg cgctcggctt ctggaagggg
     4441 tagtgggctg ttcgtgctca atggacctga acacgaatcg atgtacgatg ctccgatgaa
     4501 ccgagccgct tggttcacgg aatcgccatc cgccagccaa ccacaagcaa cgaaaagggt
     4561 gtcatgatag aaggcgatgg acatggcacc gccaccagcc tcagtaagta taccaggaac
     4621 tccgcaccat tgacttccgt cccaaaccgc gactccgtcc gatggaattc cgcccgcgta
     4681 ccggaacccc ccgcccacga atagcagccc atcatggatc cgcatggccc ggcaatcgct
     4741 gaacgtgtat gtatttccga gctcccgctg caagccaagg cccaaagcgt ggaactgtga
     4801 gccgtcccag cgcatgatgt cctgcccggc gttgccttcg cccaggcgta gctgcccgcc
     4861 cacatacagg tcgccttggt actccatgat actgtgcgca ccggaaagcc ctccttggat
     4921 ccccggcccg acaagttgcc aagtggagtc gatcagttgc atgatgcctc gaccatcctc
     4981 atttcccatg agtccaatag cggctatgct accgttgtaa ctggtcagat ccaagagctg
     5041 tgggtcgtcc tgttcaactg aattccctac tcggtgccat gccccacctc tacgtaccgc
     5101 aatacccacg atggtagtgt cgccatcgac gctatcgaaa ctgccaacag cgtacaattc
     5161 ttcgtcaatg atccgcaatc gacgcacgga aaggtcttgg aacacgccaa agggtagcca
     5221 gttgccattc acatgggccg caacgttgta catcgtatca ctattcaccg ttgtgaacgc
     5281 ccctccaaca accaacgtgt cgtgccagtg gaccaccgta gtaacaactc catgtacaac
     5341 accgagcgta tcccacccct gtgccgtcag gcgcatgatg gaattgctgg cccaccagga
     5401 aacgccgttg agttgaagcg tgccgcagta atagagcgta tcctcgttcg gcgtggcgta
     5461 gacttcctgc aacacgaccg aagtaccggg caggcctgcc gatgcccaga attgggcgtc
     5521 ggccaggtgg ggcatgagca cgaccgtagc tgcggagagt atcttccaca gtcgcctgta
     5581 caggcatttt gccgaatgcc atatgcttgg catctatcgg acaacgatat aacgccagga
     5641 tcggtgcgac gcgagaccgt gcaggatgta agcacattcg cagaggtcgg ttgtgggcgc
     5701 catggtcgcg gcggtcggat ttgtggaaag cgacagaacc gtacggtgct gcctcgtcat
     5761 aggcggttca ggcttccagc agcgcccact tcaccagccc tgtccgggcc aggaagtacc
     5821 gccagctggt gcccaccacg ccaagcccat aggtcatgct gcggctgaag ttgatggagc
     5881 tggcctcgtc gaaatacttg gtggggcagg tgatctcggc gatctcgtgg ccgttccagc
     5941 agatctgcgc cacgatctcg ttgtcgaatc gaccctccga ctgcgcctgt gccgcacgtc
     6001 gctgactctc tgcgccaaac gcgtcgaggt cctcgcgact gagcccccac ttgtcggcga
     6061 tcatctcagc ggagattccc tgcgggacga ggcccccctg gtcggcgtag cggaggccaa
     6121 cctgctcgcc gaacgggaag ccgatcctct tcatgtcgcc gaacgatgcg cccatcggca
     6181 cgaggctcat cacttcgacg ccggcagcga tggcgatgtc gtacgcgccg gccatcactc
     6241 cttgcgccgc gaaatgggct gactgctggg agcttccgca ttgccggtcg atcgtcgtcg
     6301 cgggcaccga ctcgggaaag ccggcggcga gcacggcgtt tctcccgacg ttgagggact
     6361 gggcgccgac ctgcatgacg cagcccatga tgacgtcgtc gacgagcgtc ggatcgaggt
     6421 tgttgcgctc gacgagagcc ttgagaacct cggctgcgag gtcggccgaa tgccaaccgg
     6481 agagatcgcc gtttcgcttg cctccggctg tgcggatggc gtcgacgatg actgctgtag
     6541 gcatgggcac actccggttc gaaaatcttg actgattggt caagattctt gcccccgtgc
     6601 ccgcgttggt caatccgctc gtcgccgatg acccccgagg accgccaccg ctaccattcc
     6661 gggcatctgt cacacgcccg cgcgctggcc gggcaccaaa ggggaaccac gccatggcga
     6721 cttcgaccga cgagcagctt cgcgaactca caaaaggcca gacggtccca accgagttct
     6781 tgaagacggt caccgagtac ggcagctcac gggcgttgcg atggatggaa ggggaggact
     6841 ggaaggagct cacgttctcc cagctcgccg accaggcagc tcgggtcgcg gcaggtctcg
     6901 cagccgggcg ggaagcggta ggtgcgcagc tgcttgcg
//
LOCUS       DAOQOC010000013         6867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4227_length_6867_cov_3.471814, whole genome shotgun sequence.
ACCESSION   DAOQOC010000013 DAOQOC010000000
VERSION     DAOQOC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_6867_cov_3.471814"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /gene="ade"
                     /locus_tag="PL106_00435"
     CDS             <1..1397
                     /gene="ade"
                     /locus_tag="PL106_00435"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNA31558.1"
                     /translation="VATVSDPHEIANVLGVAGVEYMIDNGAQVPFRFFFGAPSCVPAT
                     VYETAGARIDAAAIGQLLERSDVGYLAEVMDFPGVLNGDHEVMAKIAHAHRLGKPVDG
                     HAPGLRGDLAGKYIASGISTDHECFTREEALDKLAHGMRILVREGSAARNFDALIGLM
                     HDHADHLMFCTDDMHPDSLVIGHIDRLCARAVSLGHDVFNVLRAACINPVEHYRMPVG
                     QLREGDPADMILVEDLRDFKAHRTWVGGELVAEDGRSLIPRIRASQPNNFKCTPKRPK
                     DFRIPSSAEDLLVIGVLDGQLITRKLWMPPTIHDGSCVADPTKDLLKIAVVNRYHDAP
                     PSIAWITNFGLKHGAIASSVAHDSHNIVAVGTNDQDLCAAINSVIAHRGGLSLSHGDH
                     QRVLPLPVAGIMTDADAWLVADAYAELDREAKELGSTLAAPYMSLSFMALLVIPHLKL
                     SDKGLFDGDSFKLV"
     gene            complement(1556..3001)
                     /locus_tag="PL106_00440"
     CDS             complement(1556..3001)
                     /locus_tag="PL106_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNA31559.1"
                     /translation="MRKVLFTLAGPVAALLGWWGLRHLGYAQAAVAGAVLWMAVWWIS
                     EAVPLAVTSLLPVVLFPLLGITDLPKTASFYGKDVNFLFIGGFLLALGIERSGLHRRI
                     ALAIVARIGTAPSRLILGALLATGLLSMWVNSTAAVLVMLPIGLSLLNAAPEHRNLGA
                     ALALSVGYGASIGGMATPVGTPPNLVFMEQWELFFPDRPAIGFGQWMLFGVPYTLLYL
                     LLTWLLMTRVLFRMPAASTADRQELRDQVRALGRATRDEKLAAIIFCATAALWITGDR
                     IAFSDNFVFPGWRALPWKGLAALGDPAVAMIGGILLFLIPSQEKGRTLLDWDFAQARI
                     PWGVVLLIGSGFAIAGGIDATGLSTTIGHALAAWRTGTEMTQVGAIAMGVTLLSELGS
                     NTAVASLVLPVLARTAEAWQMLPQAVMIPATLGASCGFMLPIASPMQAIVFSSGHLSV
                     RRMVTAGIWMDLLGVLLLMGLWALMGHAVFG"
     gene            complement(2998..3384)
                     /locus_tag="PL106_00445"
     CDS             complement(2998..3384)
                     /locus_tag="PL106_00445"
                     /inference="COORDINATES: protein motif:HMM:NF026420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HNA31560.1"
                     /translation="MRKPLLLLGTLVFAVFAYLNLNDVDPLPWVAAYLGVAALLGLGA
                     FNIRDRRATLALAVVLLAWMCTMFPGMIDWVREGFPSIVGTMKAETPHVEVVREFLGL
                     LIAVVCLAVLWLATPRSARFTRDDNE"
     gene            complement(3391..5682)
                     /locus_tag="PL106_00450"
     CDS             complement(3391..5682)
                     /locus_tag="PL106_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA31561.1"
                     /translation="MLRYRLVTAALVLTPALFGQVTARLSGHVVDADTRHPLGGATVE
                     VLRSDSTIASASTDSSGAYRFETLAPGIVALRVRLEGHLSSTVPEVWVRTGRTELVDV
                     ELRSATRTLREVEVQENRPRHMGTIGEQVITVEQSLRYPATFFDPVRTVDTHAGIART
                     NDQANHFSVRGNGPGSNAWLLEGAEIVTPNHLTNAGTASDMPVISGGGVTILSAQMLG
                     TSRLLMGSPEVAYGDALGGIMDMRLRRGANDRAVHTVQAGLIGIDLATEGPFKKGGGA
                     SYLVNYRYSTVGLLGLMGVNLGDEAISFQDLSFHVSLPFRRGSVSLFGLGGLSSNRFE
                     AVEDSTKWEYDKDSQNINYSARMGAAGGTLKLLLGGKAVWSTTAVLSEQDQQRDADGI
                     LADNSIHSSSNRLDERKMTIVSHVRGTMGKRLGYQIGGSAMERDLFKSDGFKERTSGW
                     LVRPYAQGSFGLGAHVRAVLGLAASCWSINGSAVAEPRLSLTWDVGRRDAIMLSAGQR
                     SQLPATQNYWMRHGTTFDNTRIGLVRSQDASLAMEHAFTEGLLMRCELFVQHLLDVPV
                     IDTAAAGSYSGPQGYSMADTWDDLVLSGLKGYGTATNSGAEVSLRQRFDRGMYYHVNG
                     TVLQARTTDAFGRMYDSRWNTGFIANAVFGREFSKRKEKLTRTWGVNARANVSGGQRY
                     MPAPDEGKGPWEGRYATYKRVDLRVYLKREHAHRTGMWALDLQNAFNFRNEAFRYFDQ
                     RQRAFVMRYQLGIIPNLSYRIEF"
     gene            5848..6687
                     /locus_tag="PL106_00455"
     CDS             5848..6687
                     /locus_tag="PL106_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HNA31562.1"
                     /translation="MRVLLLFCLWLPGLAASAQQPGKHELFQRMFAAIGKVRTLTFEL
                     DQSERIGGKMMTGSQTAKLNCAPFKVYLKVLAPDPGTELLYVAGSNGGKVKLNPGRSP
                     HIVLNLDPDSGLLRKGRHHSVKEIGFGYVGGLLKNVYDRYRTNIDEWVQFKGEVVHHG
                     RTCWSITFTNGDYALRDVTTGHGENVIAVARRTFTNEYELLELNGLKNYDAVEPGRSL
                     KVPDTYFKSVDLYIDQATCLPVYQKMYDAKGVFAVYDYKDLRVNPVIAPEEFTTGYKG
                     YGF"
BASE COUNT         1352 a         2174 c         2144 g         1197 t
ORIGIN      
        1 ccgtggccac cgtgagcgac ccgcacgaga tcgcgaacgt gctcggcgtg gcaggcgtag
       61 aatacatgat cgacaatggc gcccaagtgc ccttccggtt cttcttcggt gcaccgagtt
      121 gtgtgcccgc cacggtgtac gaaacggccg gggcgcgcat cgatgcggcc gcgatcggac
      181 aactgttgga acgttccgat gtgggctacc tggccgaagt gatggacttt cccggtgtgc
      241 tcaacggcga tcacgaagtg atggcgaaga tcgcccatgc ccatcggctg ggaaaacccg
      301 tggacggcca cgcacccgga ctgaggggcg acctggctgg aaaatacatc gcgtccggta
      361 tcagcacgga tcacgaatgc ttcacccggg aagaagcgct ggacaagctc gcccatggca
      421 tgcgcatcct tgtccgcgaa gggagtgccg cccggaactt cgacgccctg atcggcctga
      481 tgcacgatca tgcggaccac ctcatgttct gcaccgatga catgcatccg gacagtctgg
      541 tgatcgggca catcgatcgg ttgtgcgcac gggccgtgtc cctcggtcac gatgtgttca
      601 acgtgcttcg tgccgcgtgc atcaatcccg tagagcatta ccgtatgccg gtgggtcagt
      661 tgcgcgaggg cgaccctgcc gacatgatcc tggtggaaga cctccgtgat ttcaaggccc
      721 accgcacctg ggtcggcggc gaactggtcg cggaggacgg gcgaagcctg atcccccgca
      781 tccgggcatc acagcctaac aacttcaagt gtacaccgaa gcgccccaag gatttccgga
      841 tcccctcatc ggcggaggac ctgctggtga tcggcgtgct cgatggacag ttgatcacac
      901 ggaaactgtg gatgccgccc acgatccacg acgggtcctg tgtggctgac cccaccaagg
      961 acctgttgaa gatcgccgtg gtgaaccgtt accatgacgc acctccatcc atcgcatgga
     1021 tcaccaactt cggcttgaaa cacggcgcca tcgccagcag tgtggcgcac gatagccaca
     1081 acatcgtggc cgtggggacc aatgaccagg acctctgcgc ggccatcaac tcggtgatcg
     1141 cgcaccgtgg aggcctatcg ctttcgcatg gtgatcacca gcgtgtgctc ccgctgccag
     1201 ttgcgggcat catgaccgat gccgacgcct ggctggtggc cgatgcgtat gcggaactgg
     1261 accgcgaggc gaaagagctc ggaagcacgc tcgctgcccc ttacatgagc ctgagcttca
     1321 tggccctcct cgtgatcccg catctcaagc tgagcgacaa ggggttgttc gacggggatt
     1381 cgttcaagct tgtctgatac cacttgttga tccttttccg ttgcggtcat cgcggccggt
     1441 ccgagcggaa cccgaacatc gatcaagtat atttcacacg accggcgatc gggccaggtg
     1501 gcatgaacgc gacgttcgga ccccggatgt gaccacacga ggcatcggat acgtgtcacc
     1561 cgaacaccgc gtgacccatc agcgcccaca ggcccatgag caaaagcaca ccgagcaggt
     1621 ccatccagat accagccgtg accatgcggc gtacactgag gtgaccactg ctgaaaacga
     1681 tggcctgcat gggtgatgcg atgggcagca tgaacccgca gctcgctccc agtgttgcag
     1741 ggatcatcac ggcttgcggc agcatctgcc acgcttcggc cgtacgcgca aggacaggca
     1801 gcaccaggct ggccacggcg gtattgctac cgagttcgct cagcagggtg acacccatgg
     1861 cgatggctcc gacctgcgtc atctccgtac cggtgcgcca cgcggcgagt gcgtggccga
     1921 tggtcgtact gagaccggtc gcatcgatac caccggcgat cgcgaagccg ctgccgatga
     1981 gcagcaccac gccccaaggg atgcgtgcct gtgcgaagtc ccagtcgagc agtgtccttc
     2041 ccttctcctg cgaagggatc aaaaagagca ggatcccgcc gatcatggcc acagccgggt
     2101 cgcccagtgc ggccagcccc ttccacggga gagcacgcca gcccgggaag acgaagttgt
     2161 ccgagaacgc gatgcggtca ccggtgatcc agagggcggc cgtagcgcag aagatgatcg
     2221 ccgccagttt ctcgtcgcgc gtggcacggc cgagggcccg gacctggtcg cgcagttctt
     2281 gccgatccgc ggtagaggca gcaggcatgc ggaacagcac gcgcgtcatc aggagccagg
     2341 tgagcaacag gtagagcagg gtgtatggca caccgaagag catccactgg ccaaaaccga
     2401 tggcgggacg atccgggaag aaaagctccc attgttccat gaacaccagg ttcggtggtg
     2461 tgcccacggg tgtggccata ccaccgatgg acgcaccata accgaccgaa agcgccagtg
     2521 cggcacccag gttccggtgt tcgggcgccg cgttcagaag gctgaggccg atgggcagca
     2581 tcaccagcac ggcggcggtg ctgttcaccc acatgctcaa cagtccggtg gcgagcaagg
     2641 cgcccaggat caggcgggac ggtgccgtac cgatccgcgc aacaatggcg agggcgatgc
     2701 ggcgatggag tccgctccgc tcgatcccaa gtgccaggag gaagccacca atgaacagga
     2761 agttcacgtc cttgccatag aacgaggccg tcttcggcag gtcagttata ccaagaagcg
     2821 ggaagagaac cacgggcagc agggaggtga cggccaaggg cacggcctcg ctgatccacc
     2881 acacggccat ccagagcacc gctcctgcca ctgccgcttg agcgtacccc agatgtcgaa
     2941 gtccccacca gcccagcagg gcggcaacgg gcccggccag ggtgaagagc accttcctca
     3001 ttcgttgtcg tcccgtgtga agcgagcgct acgcggtgtt gcgagccaca gcacggccag
     3061 gcagaccacc gcgataagca gccccaggaa ctcccgcacc acttccacgt gcggggtctc
     3121 cgccttcatg gtgcccacga tggaggggaa tccctctcgc acccagtcga tcatgcccgg
     3181 gaacatcgta cacatccagg cgagaaggac gactgcgagc gccagagtgg cccgtcggtc
     3241 gcgtatgttg aaagcaccaa ggcccaggag cgcggcaacg ccgaggtagg ccgccaccca
     3301 gggcagtgga tccacgtcgt tgaggttgag gtacgcgaac acggcaaaaa caagggtgcc
     3361 gagaaggagg agaggctttc tcatgtgggg tcagaattcg atacggtagc tgaggttggg
     3421 aatgataccg agctgatagc gcatcacaaa ggcgcgctgc cgttggtcga agtagcggaa
     3481 ggcctcgttg cggaagttga acgcgttctg caggtccagc gcccacatgc ccgtccggtg
     3541 cgcatgctcc cgcttcaggt acacccgcag atccacgcgc ttgtaggtgg catagcggcc
     3601 ttcccacggc cctttgccct catccggtgc gggcatgtaa cgctgtccac cggacacgtt
     3661 cgcgcgcgca ttgacgcccc aggtacgggt gagcttctcc ttgcgcttgc tgaactccct
     3721 gccgaagacg gcgttcgcga tgaagccggt gttccagcgg gaatcataca tccggccgaa
     3781 cgcatcggtg gtacgtgctt gtagtacagt accgttcaca tggtaataca ttccacggtc
     3841 gaagcgctgg cggagggaca cttcggcgcc gctgttggtc gcggtgccat accccttgag
     3901 tccggaaaga acaaggtcat cccaagtgtc cgccatgctg tatccctgcg gtccggaata
     3961 cgaccccgcc gccgcggtat cgatcaccgg tacatcaagg aggtgctgga cgaacaactc
     4021 acatcgcatc aggagcccct cggtgaaggc atgctccatc gcgagggacg cgtcctgcga
     4081 gcgaacgagg ccgatgcgcg tgttgtcgaa ggtggtcccg tgccgcatcc agtaattctg
     4141 ggtggccggc aactggctgc gctgcccggc tgaaagcatg atcgcgtcgc ggcggccaac
     4201 gtcccacgtg agcgagaggc gcggttccgc cacggcggag ccgttgatgg accagcacga
     4261 ggccgccaga ccgagcacgg cccgcacgtg ggcacccagg ccgaagctgc cttgggcata
     4321 cggtcgcacg agccagccgg aggtccgttc cttgaagcca tcgcttttga acaggtcccg
     4381 ttccatggca ctacctccga tctggtatcc aagccgtttc cccatggtcc ctcgcacatg
     4441 gctcacgatg gtcatcttcc gttcatccag ccggttgctg ctcgaatgga tcgagttgtc
     4501 cgcgaggatc ccgtccgcat cgcgttgctg gtcctgttcg gaaagcacgg ccgttgtgct
     4561 ccacaccgcc ttgccgccca acagcagctt caacgtcccg cccgcagcac ccatccgtgc
     4621 ggagtagttg atgttctggc tgtccttgtc gtactcccac ttcgtactgt cctccaccgc
     4681 ctcgaaccgg ttgctgctga ggccgcccag gccgaagagc gaaacggagc cgcgccggaa
     4741 cggcaacgac acatggaatg agaggtcctg gaacgagatg gcctcatcgc cgaggttcac
     4801 gcccatcagg cccagcaggc ccacggtact gtaccggtag ttcacgaggt acgaggcccc
     4861 gccacctttc ttgaagggcc cctccgtggc caggtcgatc ccgatcaatc ccgcttggac
     4921 ggtatgcacc gccctgtcgt tggcaccacg tcgcaggcgc atgtccatga tcccgcccaa
     4981 tgcatcacca taggccacct ccgggctgcc catcagcagg cgcgatgtac cgagcatctg
     5041 tgcgctgagg atggtcacac cgccaccgct gatcacgggc atatcgctgg ccgtgcccgc
     5101 gttcgtcaag tggttcggcg tcacgatctc ggcaccttcg agcagccagg cattgctgcc
     5161 gggaccgttg ccgcgcacgc tgaagtggtt ggcctggtcg ttcgtacgcg caatgcccgc
     5221 atgcgtgtcc acggtgcgta ccggatcgaa gaaggtagcc gggtaccgga gggactgttc
     5281 cacggtgatc acctgctcgc cgatggtacc catgtgacgc ggtcggtttt cctgcacctc
     5341 cacttcgcgc agggtccgtg tggcggaccg gagttccaca tccacgagtt cggtacggcc
     5401 ggtgcgcacc cacacctcag gcacggtgga cgaaagatga ccttcaaggc ggacacgaag
     5461 cgcaacgatg ccgggggcca acgtctcgaa gcggtaggct ccggagctgt cggtcgaagc
     5521 cgaggcgatg gtgctgtccg agcgaagcac ctccacggta gccccgccca gggggtggcg
     5581 tgtatcggca tccaccacat ggccgctgag ccgagccgtc acctgtccga agagtgccgg
     5641 ggtcagcaca agggccgccg tcaccaggcg gtaccgtagc atgtcgcgaa cttatccgga
     5701 acccatgggc accggaccgc cggttcgcca agtatcaccg atcgcgttgt tcattaccga
     5761 tcaggggccg ctggtccggt tccggggcca tgcggatcct gccgcctgtt ccactggcat
     5821 agttttggtg cgcaccccgc tccctcgatg cgcgtcctcc ttttgttctg cctctggctg
     5881 cccggccttg cagcgtcggc acagcagccg ggcaagcacg agctcttcca gcggatgttc
     5941 gcggccatcg gcaaggtccg caccttgacc tttgaactgg accagtcgga gcgcatcggc
     6001 gggaaaatga tgaccggctc gcagaccgcg aaactgaatt gcgcaccatt caaggtctac
     6061 ttgaaagtgc tggcaccgga tcccggcacc gaattgttgt acgtagcagg tagcaatggc
     6121 ggcaaggtga agttgaaccc cggccgctcg ccgcacatcg tattgaacct ggacccggac
     6181 agcggcctgc tgcggaaggg acggcaccac agcgtgaagg agatcggttt cggatacgtg
     6241 ggcgggttgc tcaagaacgt gtacgaccgt tatcgaacaa atattgatga atgggtacaa
     6301 ttcaaaggtg aggtggtcca tcacggccgc acgtgctgga gcatcacgtt caccaacggc
     6361 gattacgccc tgcgcgatgt gaccacaggc cacggcgaga acgtgatcgc ggtggcacgg
     6421 cgcaccttca ccaacgagta tgaactcctg gagttgaacg gcctgaagaa ctacgatgcg
     6481 gtggagccgg gccggtcgct gaaggtgcct gatacatatt tcaagagcgt tgacctgtac
     6541 atcgaccagg ccacctgcct gccggtgtac cagaagatgt acgacgccaa gggcgtgttc
     6601 gcggtatacg actacaagga cctgcgtgtg aacccggtga tcgcaccgga ggaattcact
     6661 acaggataca agggatacgg gttctgaggt agtgctcctc gtcagccgac cgggtccttg
     6721 cgcatgacga tgatggtctc attgcggttc acggggttga gcgcgtgtac cacgcgatca
     6781 agcaggccgg gccgggccac ggccaccaca tgcagcgggc ccatggcccg tgtgatcctg
     6841 gccatgcgtt ccgccagatc ctccatc
//
LOCUS       DAOQOC010000014         6831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4259_length_6831_cov_4.356405, whole genome shotgun sequence.
ACCESSION   DAOQOC010000014 DAOQOC010000000
VERSION     DAOQOC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_6831_cov_4.356405"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PL106_00460"
     CDS             complement(<1..1453)
                     /locus_tag="PL106_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNA31563.1"
                     /translation="MLSAQVDSIRLKYGNTITQEDLRKHLEVLASDAYEGRETGAQGQ
                     KMAASYIREQFVSYGIPPVPDAEEKGLINGYEQPFPLELTRPGGLSLIVNGTPIGFMQ
                     EFFYLSERLREDYSTDKVVFAGMGDQDPGPDARGGTVLVLDKGSGTTATDRPSRELFQ
                     RITRLAAMAEQAGARVLLIASPDTREMMRQFAHYITQPRMRLVEEKAEAAPARTGIQT
                     IILDKATAERMLRTGRMTWKKALRSAQKPPRTLPCSFAARFVPNEQRLQGENVLGYVE
                     GSDKKDELVVVTAHYDHIGTDHEDVYNGADDDGSGTVAVLEMAEAFAQAKRDGHGPRR
                     SVLFMPVSGEEKGLLGSEWYSEHPVFPLESTVADLNIDMIGRTDSAHAHGAPYVYIIG
                     SDRLSTELHRINEAANATYTHLELDNTFNAADDPNRFYYRSDHYNFAKHGVPVIFYFS
                     GVHEDYHQPGDEVDKIRFDLLQQQARLVFYTAWE"
     gene            complement(1514..1960)
                     /gene="rpiB"
                     /locus_tag="PL106_00465"
     CDS             complement(1514..1960)
                     /gene="rpiB"
                     /locus_tag="PL106_00465"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNA31564.1"
                     /translation="MRISIGGDHAGYGLKEVVIGHLKSQGIDVHDQGTHSSDSTDYPD
                     YAHAVASDVESGSADLGIVICGSANGVNITANKHHNVRSAIAWNEDVAKLAREHNDAN
                     VLALPARFVDEALALRIVDVFLRAHFEGGRHQRRVDKIEPQPVRRS"
     gene            2435..3925
                     /locus_tag="PL106_00470"
     CDS             2435..3925
                     /locus_tag="PL106_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNA31565.1"
                     /translation="MCVTGLCMGQNGFTQYRYPNGQVSSEGHLVNGTPEGYWKSWYEN
                     GVIKSEGNRTSSLLDSTWKFYDESGKLTTEIAYAGGKKNGLTRKFGEDGSLVSEEPFV
                     NDVKEGEARTYYPNGALHERSSFKQGVEDGKAYEYATDGRIITIYDHRAGMLRSKETL
                     NRFDEQGWKQGFWREYWPNGKPKLEGRYVDDKRQGIFKEYDVNGALKTMEKFDQDEIM
                     KDAEEVKMLAIKNTYHSSGKVASIGSYSKDGKKEGLFRNFGEDGKPTTAAIYQSGQKM
                     SEGAVNDVGALQGNWTEYYGSGEKRAEGGYKDGKKDGPWTFYHRTGEVEQRGNYVNGL
                     PQGEWVWFFPSGARHREEHYRKGKEDGASVEYDSTGAVITQGEYIDGLKDGPWIYHVG
                     DHTETGAYKDGLRDGEWKHMYGNGKRAFVGRYVNGEPAGKHRWWWPNGQLMMEGKYVA
                     GLASGDFDQYDELGRPVITIRYRAGAEMKLDDAQLPPPYEPGGPTD"
     gene            3959..>6831
                     /locus_tag="PL106_00475"
     CDS             3959..>6831
                     /locus_tag="PL106_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNA31566.1"
                     /translation="MAETNGTGSLISQGTELASAYLDLKNQYDLLVRKNLAGVFRTTV
                     QGRFVECNDAMARMLGYKDREALMSVHAGDLYVEADARQRFLDDLRKHGQLVNYEIAL
                     KRIDGRTVHVLENVFLNEQADRSTTIDGTLIDITAIRQGELEQRALLNNYRQLMDRVR
                     DAILIVRDDRVLYANPSAEALVSEHEWLGRSFIERLHVDDRATVQEAIDLHGSGSSSG
                     IIDVRVDAGSGTWRDMLLSAAVLQHEGGIATQVTLQDVGLHRSLVHERSRARMAEEVN
                     EILRQEIIEHRRTQEALRQSRRFARSLVDSSLDTIIAVDQQGRITEFNPAAAIKFGYD
                     ADEVMGQRTDMLYADEAEYKRVQQEMNAHGAFAGEVINITRDGRAFTCFLVSSRLYDE
                     DGKLLGTMGVGRDITQIKRDQEALRASEERYRDLFENATDLIQSVDIEGVFQYANDTW
                     CRTLGYTKEEVGQLRIEKIIHPDDLAEFTQWRVKLFAGEQPVPITVRFRAKDGRTVIV
                     LGSSTVRSVEGRPVATRSIFRDITAEQTALEEVQEHEAQLRALFESSEHMFWSVDRRI
                     AITSFNRGYGDMIERLYGIRPEVNTDPTTPRKRFASAAYHAFWEEKYAEAFNGKAMRF
                     ETDVTDREGRRVCNEIFLSPVFGIDGTVDEVFGVGHEITEQKEAEDLVREQAARLKAI
                     FENSANMMIWTLDHDYRITSMNEHFVLSNERAMGIRFELGDNFIKHLMDRVADKRYQP
                     VVDKFAMALSGKPQQFEVELKNHAGRSLWVENFLNPITLDGQVVEISCQAFGITDKKE
                     SQRKLLDSLHEKEVLLKEVHHRVKNNLQIISSIFNLQKQYVDDHPRTLELLRDSQDRI
                     RSMSFIHESLYQTKNFSHVDLAAYIEGLTRNLMMSYSLNGKVELELDLRPVELVLDQA
                     IPCGLILNELIGNALKHGFPDGAAGRIAIGLHADGDQVRIRVT"
BASE COUNT         1502 a         1926 c         2083 g         1320 t
ORIGIN      
        1 attcccaagc cgtgtaaaag acgagccggg cctgctgctg gagcaggtcg aagcggatct
       61 tgtccacctc gtcgcccggc tggtggtagt cctcatgcac accgctgaag tagaagatca
      121 ccggtacacc gtgcttggcg aaattatagt ggtcgctacg gtagtaaaat cgattggggt
      181 cgtcagcggc attaaaggtg ttgtccagtt cgaggtgggt gtacgtggcg ttcgcggctt
      241 cgttgatccg gtgcaattcg gtgctcaggc ggtcactgcc gatgatgtac acgtacggtg
      301 caccatgcgc gtgggcacta tccgttcgac cgatcatgtc gatgttcaga tcggccaccg
      361 tgctctccaa cgggaagacc ggatgctcgc tgtaccattc gctgcccagc aggcccttct
      421 cctccccact gacgggcatg aagagcacgc tgcgccgcgg accgtgccca tcgcgctttg
      481 cttgggcgaa cgcctcggcc atttccagaa cggccaccgt tcccgagcca tcatcatccg
      541 cgccattgta cacatcctcg tggtccgtgc caatatggtc gtagtgcgcc gttaccacaa
      601 ccagttcgtc cttcttgtcg ctgccttcca cataacccag gacattctcc ccttgcagac
      661 gttgttcgtt gggtacgaac cgcgcggcga atgagcaggg caacgttcgg gggggcttct
      721 gtgcggaacg cagtgccttc ttccacgtca tgcggcccgt tcgcagcatc cgctcggctg
      781 tggccttgtc caggatgatc gtctgtatcc cggtccgggc aggtgcggcc tcggctttct
      841 cctccaccaa tctcatccgg ggttgggtga tgtaatgtgc gaactgcctc atcatctccc
      901 gcgtgtccgg actggcgatc agcaacaccc gggcacctgc ctgttcggcc atagcggcca
      961 ggcgtgtgat ccgctgaaaa agttctcgcg agggccggtc cgtagcggtg gttccggatc
     1021 ctttgtccag cacgagcact gttccgcccc gggcatccgg accgggatct tgatcaccca
     1081 tacccgcgaa aaccaccttg tccgtggaat agtcctcgcg cagccgctcg ctcaggtaga
     1141 aaaactcctg catgaagccg atgggagtcc cattcacgat gagcgagagt ccgccagggc
     1201 gtgtcaattc aagcgggaag ggttgctcgt atccgttgat caggcctttc tcctcggcat
     1261 ccggtaccgg tgggataccg taagatacaa attgttcccg gatgtaactg gccgccatct
     1321 tctggccttg cgcaccggtc tcccgacctt cataggcatc actggccagc acttccaggt
     1381 gctttcgcag gtcctcctgt gtgatcgtgt tgccgtactt cagccggatg ctgtccacct
     1441 gggcagacag catgcatggt gcacagatgc agatcccgac gaacgagcgc agcatgtatc
     1501 ccatgggtcc tgttcaggag cgacgaactg gctgcggttc gatcttgtcc acgcgacgct
     1561 ggtgccgacc gccctcgaaa tgcgccctaa ggaacacgtc cacgatacgc agggccaagg
     1621 cttcatccac gaatcgtgcg ggcagggcca gcacgttggc atcgttatgc tcacgggcca
     1681 atttggccac gtcctcgttc cacgcgatgg ccgatcgcac gttgtgatgc ttgttggccg
     1741 tgatgttcac accattggca ctgccgcaga tcacgatgcc cagatccgcc gagccgcttt
     1801 ccacatcgct cgccaccgca tgcgcgtagt ccgggtagtc cgtgctgtcc gaactgtgcg
     1861 ttccttgatc atgtacgtcg atcccttggc tcttcaagtg gccgataacc acctccttca
     1921 atccgtaacc ggcatgatca cctccgatgc tgatgcgcat gtgtttagcc gccaaggggt
     1981 tcaatttcca gcgatgcacc aaaggtagaa gcatcaccct ttccaccgtg cgatcgtgaa
     2041 cagcccattc cacttgttaa caatgggcgg caactttccc cttatcaaca caccatcccc
     2101 atcccttgat ccttttcacc ggaactccaa accgacttgt tgttcttctg ccaacaggga
     2161 tcgaacgatc cgaccttgcc ggaaccggaa cgatgatcca acgatcaccc gcatcccaac
     2221 gatccaacga tcgttcattc cgatggagca acccgtaact gtccaaggat ggccgttgct
     2281 caaccggttc accggatgtt ggcaacccgt gggctatttt tgaaaactcg gaatgtcaag
     2341 tggacacgcc atggatcatc aaccgatcca acagcacata ttacaacatc agatcttttt
     2401 gaaaaaaaag agaatacaaa tactttgttc gttcgtgtgt gtgacgggcc tgtgcatggg
     2461 gcagaacggc ttcacgcagt accggtaccc gaacggacaa gtgagcagcg agggccactt
     2521 ggtcaatggc actcccgagg gctattggaa gagctggtac gagaacgggg tgatcaagag
     2581 cgaagggaac cggacctcct ctttgttgga cagcacatgg aagttctatg atgagtcagg
     2641 gaagctgacc acggagatcg cctatgcggg cggcaagaag aacggtctga cacgcaaatt
     2701 cggtgaggat gggtctttgg tgagtgaaga accgttcgtg aacgacgtga aggaagggga
     2761 ggcacggacc tactatccga acggcgcgtt gcatgagcgg agttccttca agcagggcgt
     2821 agaggatggc aaggcgtacg aatacgccac tgatggacgg atcatcacca tttacgatca
     2881 tcgggcgggc atgctgcgct ccaaggagac actgaaccgg ttcgacgaac aaggctggaa
     2941 acaaggtttc tggcgcgagt actggcccaa tggcaagccc aagttggagg ggaggtacgt
     3001 ggacgacaag cgccagggga tcttcaagga gtacgatgtg aatggtgcgc tgaagaccat
     3061 ggagaagttc gatcaggatg agatcatgaa agatgctgag gaggtcaaga tgctggccat
     3121 caagaacacc tatcattcct cagggaaagt ggccagcatc ggcagctatt ccaaggacgg
     3181 caagaaggag ggtctgttca ggaatttcgg tgaggatggg aagccaacga ccgcggccat
     3241 ttaccagtcc ggacaaaaga tgagcgaggg tgctgtgaat gacgtgggtg ccctgcaagg
     3301 caactggacc gagtactatg gcagcgggga gaaacgggcc gagggcggat acaaggatgg
     3361 taagaaggat ggcccgtgga ctttctacca tcggacgggg gaagtagagc aacgtggcaa
     3421 ctacgtgaat gggttgcccc agggcgagtg ggtctggttt tttccctctg gagcgcggca
     3481 tcgcgaggaa cactaccgca agggcaagga ggatggggca tcggtggaat acgacagcac
     3541 aggggcggtg atcacccaag gcgagtacat cgacgggctg aaggatggtc catggatcta
     3601 ccacgtgggc gaccacacgg agaccggagc atacaaggat ggtttgcgcg atggcgaatg
     3661 gaaacacatg tacgggaatg gaaagcgcgc tttcgtggga cgctacgtga acggtgagcc
     3721 cgccggcaaa caccgctggt ggtggccgaa cggacaactc atgatggaag gcaagtacgt
     3781 ggcgggcctg gccagcggcg acttcgatca gtacgatgaa ctgggtcgcc cggtgatcac
     3841 catccggtac cgcgccggtg ccgagatgaa gttggatgac gcccaattgc cccctccgta
     3901 cgagcccggt gggcctaccg attgatcgcg cagcactgcg ggtacgtaag ttagagccgt
     3961 ggcggaaacc aacgggacag gatcgctcat cagtcagggc acggagctgg caagcgccta
     4021 cttggacctg aagaaccagt acgatctgct ggtccggaag aatcttgccg gggtgttccg
     4081 caccaccgtg caaggtcgat tcgtggagtg caacgacgcc atggcccgta tgctcgggta
     4141 caaggaccgt gaggccctga tgtccgtcca tgcaggcgac ctttatgtgg aggccgatgc
     4201 tcgccaacgc ttcctggatg atctgcggaa acacgggcag ctggtgaatt acgagatcgc
     4261 gctgaagagg atcgatggtc gcacggtgca tgtgctggag aacgtattcc tgaatgaaca
     4321 ggccgaccga tccaccacga tcgacggcac gctcatcgac atcacggcca tccgtcaggg
     4381 cgaattggaa caacgtgcgc tgctgaacaa ctacagacag ttgatggatc gcgtgcgtga
     4441 cgccattctc atcgtgcgcg atgatcgggt gttgtacgcg aacccgtccg ccgaggcatt
     4501 ggtgtccgaa cacgaatggc taggacgatc tttcatcgaa cgcttgcacg tggatgatcg
     4561 ggccacggta caagaggcga tcgatctcca tggatcgggg tcatcatccg ggatcatcga
     4621 tgtgcgcgtg gatgcgggaa gcggtacttg gcgcgatatg ttgctgagcg cggcggtcct
     4681 gcaacacgag gggggtatcg ccacgcaggt gaccctgcag gatgtgggcc tgcaccgctc
     4741 gctggtacac gaacgcagcc gcgcccgtat ggccgaagag gtgaatgaga tcctccgtca
     4801 ggagatcatc gagcaccgcc gcacccaaga agccttgcgc caaagccgga ggttcgcgcg
     4861 gagtctggtg gacagctcct tggataccat catcgcggtg gatcagcagg ggcgcatcac
     4921 cgaattcaat ccggccgccg ccatcaaatt cggttatgat gccgatgagg tgatggggca
     4981 acgcacggac atgttgtacg cggatgaggc ggaatacaaa cgcgttcaac aggagatgaa
     5041 cgcgcatggc gcattcgccg gggaggtcat caacatcacg cgcgacggtc gggcgttcac
     5101 gtgcttcctg gtcagcagtc gcctctacga tgaggatggc aagctgctcg gcaccatggg
     5161 cgtgggccgc gacatcacac agatcaaacg cgatcaggag gcattacgtg cgagcgagga
     5221 gcggtacagg gatcttttcg agaacgccac ggacctgatc cagagcgtgg atatcgaggg
     5281 ggtgttccag tacgccaatg acacctggtg ccgcaccctc gggtacacca aggaggaggt
     5341 ggggcaattg cggatcgaaa agatcatcca ccccgatgac ctggcggagt tcacgcaatg
     5401 gcgggtgaag ctcttcgcgg gagagcaacc ggtgccgatc accgtacgat tccgggccaa
     5461 agacggacgc accgtgatcg tgctgggcag cagcaccgtg cgttcggtcg aggggcgccc
     5521 cgtggcgacc cgcagcatct tccgcgacat cacggccgag cagaccgcgc ttgaggaggt
     5581 gcaagaacac gaggcccaac tgcgcgccct tttcgagagc agcgaacaca tgttctggtc
     5641 ggtggatcga cgcatcgcca ttaccagctt caatcgcggt tacggggata tgatcgaacg
     5701 cctgtacggt attcgacccg aggtgaacac cgaccccacc acaccccgca aacgcttcgc
     5761 cagcgcggcc taccacgctt tttgggagga gaagtacgcc gaggcgttca acgggaaggc
     5821 catgcgattc gagaccgatg tgaccgatcg ggagggaaga cgcgtctgca acgagatctt
     5881 cctgagccct gttttcggga tcgatggcac ggtggatgag gttttcgggg tcgggcacga
     5941 gatcacggaa cagaaggagg ccgaggacct tgttcgcgaa caggctgctc gtctgaaggc
     6001 catcttcgag aacagcgcca acatgatgat ctggacgctt gaccacgatt atcggatcac
     6061 gtccatgaac gagcatttcg ttctcagcaa cgagcgtgcg atggggatcc ggttcgagct
     6121 gggcgacaat ttcatcaagc atctcatgga ccgtgttgcg gacaagcgct accagcccgt
     6181 ggtggacaag ttcgccatgg cgctcagcgg aaaaccgcag caattcgagg tggaattgaa
     6241 gaaccacgcc ggccgttcgc tctgggtgga gaacttcctc aacccgatca ccctggacgg
     6301 ccaggtcgtg gagatcagct gccaggcttt cggtataacc gacaagaagg agagccagcg
     6361 caaattgctg gacagtctcc atgaaaagga ggtgctcctc aaggaggtac atcatcgggt
     6421 gaagaacaac ctgcagatca tctccagcat tttcaacctg cagaagcagt atgtggacga
     6481 ccacccgcgc acgctcgagt tgctgcgcga cagccaggac cgcatccgca gcatgtcctt
     6541 catccacgaa agcctgtacc agaccaagaa tttcagccat gtggacctgg ccgcctatat
     6601 cgagggtctc accaggaatc tgatgatgag ctacagcttg aacgggaaag ttgaactcga
     6661 gctggatctg cggcctgttg aactcgtgtt ggaccaggcc atcccctgcg gactcattct
     6721 caacgaactc atcgggaacg ccctcaagca cggctttccc gacggggccg ccggtcgcat
     6781 cgccattggc ctgcatgccg acggggacca ggtgcggatc cgggtgaccg a
//
LOCUS       DAOQOC010000015         6630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4449_length_6630_cov_3.428137, whole genome shotgun sequence.
ACCESSION   DAOQOC010000015 DAOQOC010000000
VERSION     DAOQOC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_6630_cov_3.428137"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..354
                     /locus_tag="PL106_00480"
     CDS             130..354
                     /locus_tag="PL106_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNA31567.1"
                     /translation="MRVHLFGLIAERAGTNLLDVEATTVDELRRVLTGRIEGLDRLSY
                     AIAVDRRLVKADMPLTGEEEVALLPPFAGG"
     gene            391..1506
                     /locus_tag="PL106_00485"
     CDS             391..1506
                     /locus_tag="PL106_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNA31568.1"
                     /translation="MIGTGLIDQADPSIWLPTFAPQPSPSGIGAQRMVNGKEAVVEEF
                     VLHRLATDDAPGILNDFSAVLEGGEEQEFLRRLFLKPFANMGETFSFADGEDGDVFHG
                     LCAGIHAGRDLVESSRSIAQRLQEVLRQHGLRGGDLFVVKFSGVELGAARHAAVGVLK
                     FDGREVFMENKPEDGRLVMRLGRGLGTLKPVLAVLVVFTPDAPTLFTIDDEEHASAWR
                     HEFIGLCRKQDHVNSTRDAMAMTRSFITDELPSRHEVDRAGQIDLLNRSVQYFKENAA
                     YDRDHFAQEVFGEKEVMSAFDQYSERYGQENDVRLQDRFDISSHAVKSQARVFKSVLK
                     LDKNFHIYIHGDRSRIEHGVDERTGRKFYKIWYDEER"
     gene            complement(1512..2462)
                     /locus_tag="PL106_00490"
     CDS             complement(1512..2462)
                     /locus_tag="PL106_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA31569.1"
                     /translation="MNPTSKSATLLILIALLTACVPARKYEELQSRYKSTQESETAAL
                     AKAQTAEAAMKEQSASVEDLTKRKANLERDTTIQGTSLRNMTVQYDKINKLNTELLEK
                     YNALLAGESSENRKLLTDLEATRLRLQRQEDSLSALARSLGDREAKLADLQKELAAKD
                     AAMKSLKDRVSAALAGFEGKGLTVTQKDGKIYVNLDNKLLFKSGSTVVESEGKQALGS
                     LGKAIENEKDLAILVEGHTDTDKIAPGSSMKDNWDLSVLRATSVVRILQESSRIDPKR
                     ITAGGRGEYVPVDPADKAKNRRIEVILAPDLAALYKLVQD"
     gene            2605..3321
                     /locus_tag="PL106_00495"
     CDS             2605..3321
                     /locus_tag="PL106_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31570.1"
                     /translation="MRNRDLAPWFSLLADEEAGHYRVLAYVHRIDGRYRERKLYPYLD
                     ELRAGAEELLSLRQEREQLASAMPRPIIGMDLLKGRLLRATVREDDILRAIDGMIANT
                     LPELDRVMERGRQLREQLTAGIRFEPVGLLPLYTNEGYLILHHGREARSYAYSLGLPM
                     PDTEGSEHRVLRTRFVADHTIGITCTYEQVKADLVRNHPGLPNPAVFAFVSDVSLPII
                     ETFVPLAKQLVYEMVAGQVA"
     gene            complement(3332..5050)
                     /locus_tag="PL106_00500"
     CDS             complement(3332..5050)
                     /locus_tag="PL106_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA31571.1"
                     /translation="MRAGPLVLSLLVLLSACDDRPQRTVSAEGRYGGVFNLNVSGAIR
                     SIFPPTITQASEVRIMGQVYEGLVSFDPATMATEPCLADSWEVDTTHTIWTFHLRQGV
                     RFHDDPAFTDGIGRTLTADDVVRCFTAICTQGIGDGTRWLFQDRVVGATEHYLASRNG
                     QPLPQGVEGIRKVDDSTVRITLTHPDPNILRIIAHQGCWIWPQELTEAYARDINTHAI
                     GTGAFRLRGPHTGGTMVLERNPVYWRQDAEGRALPYLDAVRITFDPDRNAEFDQFLKG
                     RVTALFELPVDRLSALKDSVDPATGEKRFTFLSVPGYASQFYCFDIHHPPFNDVRVRK
                     AFALAVDRRTLVDQVLGGLASPADHGIVAPGLKNYPYNLVRGFHFDPDSARLLLASAG
                     YPQGRGFPPLRLQVSADGFGYVRVAEAVLGMIERELNVPITLSVLPAEQHLALTEHLK
                     ARFWREGWSADHPDPENFLAMLYGKNAAQDTALASPLNTLRYHSRAFDTRFEQAIHEP
                     DENVRSRLLAEAENIAMNDVPAIPLYHDRSVLLTSPRVMNLRPNPLDLMDLGTVWFNH
                     KQVPAQ"
     gene            5200..>6630
                     /locus_tag="PL106_00505"
     CDS             5200..>6630
                     /locus_tag="PL106_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31572.1"
                     /translation="MPSVSSPMHYTWRYPAAVFLVLAMTGPAVAQELDNRRERRIVLD
                     RDTVRLDSMSIAPGSLTLWMDSVALDTAFYRNDPWHGLILRRPGAPIDTLIARYRVLP
                     LLLPGPFHHKDIRLRTAAMADRTDPFKYVPGEGKEDPLGIHGLTKSGSISRGVLFGNN
                     QDLSVNSALNLELSGKLTERIGVLASITDNNIPIQAGGNTAELQDFDRVFIKLFDERQ
                     ELIAGDFVLERPKSHFLTYYKKSKGLGYSTLLGKDPLDTLSRKARGTFAVSAAISKGK
                     FARNVIQGVEGVQGPYRLSATDGGAFIIVLSGTERVFINGQLMARGQENDYVIDYNTA
                     ELTFTARRLITKDRRITVEFQYSDKNYARSQVRFSNSWSIGRTDLYADLYTEQDHKNQ
                     PLQQTLSDADRLVLSQAGDDPLAAAAPGIDSTGWNPDEVLYLRRDSLGYFPVFVHSTD
                     SVNARFRVSFSNVGPGNGDYVQEEFTP"
BASE COUNT         1231 a         2023 c         2058 g         1318 t
ORIGIN      
        1 gatggcaacc tgaaaatgac cgaagcgctc accgcgatca ataccgcgtg gcttgccgtt
       61 cagccgggcg aatacggctg gcccgccgat ctgttccgca acttgaacgc gctgagcgat
      121 ctttgagcca tgcgggtcca tctgttcggt ttgatcgcgg aacgtgctgg cacgaacctt
      181 ctggacgtgg aggcgaccac ggtggatgaa ctcaggcgtg tcctgaccgg gcgtattgag
      241 ggcctggatc gattgagtta tgccatagcc gtggatcgcc gcctggtgaa ggcggatatg
      301 ccgcttacag gtgaggagga ggtcgccttg ttgcctccgt tcgctggcgg gtgaccagtc
      361 ctccggggcg gccatagcac tggcccatcc atgatcggga ccgggctgat cgaccaggcc
      421 gatccaagca tctggcttcc gaccttcgcc ccgcagcctt cgccatcagg catcggcgca
      481 caacgcatgg tgaacggcaa ggaagcggtc gtggaggaat tcgtgctcca tcggctcgcc
      541 acggacgatg cccctggcat tctgaatgat ttcagcgcgg tgctcgaagg tggtgaggaa
      601 caggagttcc tgcgccgact ttttctgaag ccgttcgcca acatgggaga gaccttcagc
      661 ttcgccgacg gagaggatgg tgatgtgttc catggactgt gcgcgggcat ccatgcgggt
      721 cgtgatctgg tggagtcctc ccggagcatt gcccagcgtc ttcaggaggt gcttcgtcag
      781 catggtttgc gtggtgggga tcttttcgtg gtgaagttca gcggtgtcga actcggcgcc
      841 gcgcgtcatg ccgccgtggg tgtgctcaag ttcgatggcc gtgaggtctt catggagaac
      901 aagcccgagg atggccgctt ggtgatgcgc ctgggccggg gcctgggcac cctgaagccg
      961 gttctggccg ttctggtcgt gttcacgccc gatgcgccta cgctcttcac catcgatgac
     1021 gaggagcacg caagcgcatg gcgtcacgag ttcatcggcc tgtgccgcaa gcaggaccat
     1081 gtgaacagca cacgggatgc gatggccatg acgcgctcct tcatcaccga cgagctgccc
     1141 tcaaggcatg aggtggaccg tgctgggcag atcgatctgc tcaaccggtc ggtacaatat
     1201 ttcaaggaga atgccgcgta tgaccgggac cacttcgccc aggaggtgtt cggcgagaag
     1261 gaggtgatga gcgccttcga ccagtacagc gagcgctatg gtcaggagaa tgacgttcgc
     1321 ttgcaggacc ggttcgacat ctcatcgcac gccgtgaaga gccaggcccg cgtgttcaag
     1381 agcgtactga aattggacaa gaatttccat atctacatcc acggcgaccg cagccgcatc
     1441 gagcacggcg tggacgagcg tacggggcgg aaattctaca agatctggta tgacgaggag
     1501 cggtagtgcg ctcagtcctg caccagcttg tacaacgcgg ccaggtccgg ggcaaggatc
     1561 acttcgatcc ggcggttctt ggccttgtct gccggatcga caggtacata ttcgccacgc
     1621 ccgcccgcgg tgatccgctt ggggtcgatg cggctgctct cctgcaggat gcgcaccacg
     1681 ctggtggcac ggagcacgct caggtcccag ttgtccttca tgctgctgcc cggggcgatc
     1741 ttgtccgtgt ccgtgtgtcc ctccaccagg atggcgaggt ccttctcgtt ctcgatggcc
     1801 ttgccgaggc ttcccagcgc ttgtttccct tcgctttcca ccacggtact gccgcttttg
     1861 aagagcagct tgttgtccaa gttcacgtag atcttgccgt ccttctgtgt tacggtgagc
     1921 cccttgccct cgaaaccggc gagcgccgca ctcacccggt ccttcaaact cttcatggcg
     1981 gcgtccttcg cggcaagctc cttctgcagg tcggccagtt tcgcctcgcg atccccaagt
     2041 gagcgcgcca gtgcactgag cgaatcctcc tgccgctgca agcgcagacg ggtggcctcc
     2101 aggtcggtga gcagtttgcg attctcgctg ctctccccgg ccagcaatgc gttgtacttc
     2161 tccagtaatt cggtgttcag cttattgatc ttgtcgtatt gcacggtcat gttgcgcaaa
     2221 ctggtccctt ggatggtggt atcccgctcc aagttcgcct tgcgcttggt caggtcctcc
     2281 accgaggcgc tttgctcctt catggccgct tcggctgtct gggccttggc caacgccgcg
     2341 gtctcgctct cctgtgtgct cttgtagcgg ctctgcaact cctcgtattt cctggcagga
     2401 acgcaggccg tcaacaacgc gatgaggatg agcagggtgg cggacttgga ggtcgggttc
     2461 atgtgtatgt aactttccgg ttgtcgcggc gaaggtatcc ctctccaccg atgcgggcat
     2521 tgaacggtag gcatgggccc acttcacccg cttgatccgg accggaacgg aaccttgtcc
     2581 gtgcggacaa cgttatccaa gcccatgcgc aacagggacc tcgccccttg gttctctctg
     2641 ctcgccgatg aggaggccgg ccattatcgt gtgctcgcat acgtgcatcg gatcgacggg
     2701 cgctatcgcg aacgcaaatt gtacccgtac ctggatgaac tcagggccgg tgccgaggaa
     2761 ttgctctcgc tgcggcagga acgtgagcag ttggcgtcgg ccatgccgcg cccgatcatc
     2821 ggcatggacc tgttgaaggg tcggctgttg cgagcgaccg tgcgtgagga tgacatcctg
     2881 cgcgccattg acggcatgat cgccaatacc ttgcccgagt tggatcgcgt catggaacgc
     2941 gggcgccagt tgcgcgagca actcaccgct ggtatccgct tcgaacccgt gggcctgtta
     3001 ccgctgtaca cgaacgaagg ttacctgatc cttcaccatg gtcgtgaggc gcgttcctac
     3061 gcctattcac tcggcctgcc catgcccgat accgagggtt cggaacaccg cgtgttgcgt
     3121 acacgtttcg tggccgacca cacgatcggg atcacctgta cgtacgagca agtgaaggcc
     3181 gatctggtgc gcaaccatcc gggattgccg aatccggccg tgttcgcctt cgtgagcgac
     3241 gtgtccctgc ccatcatcga gacattcgtg ccactggcga agcaattggt ttacgagatg
     3301 gtcgccgggc aggtggcata acgccacccg gttactgtgc cgggacctgc ttgtggttga
     3361 accacacggt cccgaggtcc atgaggtcca acgggttggg tcgaaggttc atgacccgtg
     3421 gcgaagtgag cagcacggaa cggtcgtgat acagggggat cgcgggcaca tcgttcatgg
     3481 cgatgttctc ggcttcggcg agcaggcgcg aacggacgtt ctcgtccggc tcgtgtatgg
     3541 cctgttcaaa ccgcgtgtcg aacgcgcgtg aatgatagcg cagcgtgttg agtggcgagg
     3601 ccagggccgt gtcctgggcg gcgttcttgc cgtacagcat ggccagaaag ttctccggat
     3661 caggatgatc ggccgaccat ccttcccgcc agaagcgggc tttcaggtgc tcggtgagtg
     3721 ccagatgctg ctctgcgggg agtacgctga gcgttatggg cacattcaac tcgcgctcga
     3781 tcatgcccag taccgcctcg gccacgcgca cgtaaccgaa gccatcggca ctgacctgca
     3841 atcgaagcgg gggaaatcca cgtccttggg gatatccggc actggccagc agcaggcggg
     3901 cgctatccgg atcgaaatgg aagccgcgga ccaaattata tggataattt ttgagcccgg
     3961 gggccacgat cccgtgatcc gccggcgagg ccaagccccc gagcacctga tccaccaacg
     4021 ttcgccgatc aacggccaag gcgaatgcct tgcgcacgcg cacatcgttg aacggggggt
     4081 ggtgtatatc gaagcagtag aattgtgatg cgtagccggg caccgacagg aatgtgaacc
     4141 gtttttcccc ggtggcggga tcgaccgaat ccttcaacgc tgacaaccgg tccaccggca
     4201 gctcgaacaa cgctgtcacc cgccccttga ggaattgatc gaactccgca ttgcggtccg
     4261 gatcaaaggt gatgcggacc gcgtcgagat agggcagtgc ccggccctcg gcatcctggc
     4321 gccaataaac cggattccgt tccagcacca tggtcccacc ggtatgcggc ccacgcaagc
     4381 gaaaagcccc ggtgccgatg gcgtgggtgt tgatgtccct ggcgtaggct tcggtgagtt
     4441 cctgcggcca gatccagcaa ccctgatgcg cgatgatccg caggatgttc ggatcgggat
     4501 gtgtgagcgt gatgcgcaca gtggagtcgt ccaccttgcg gatgccctca actccctgcg
     4561 gcaagggttg cccgttccgg ctggcgaggt aatgctccgt ggcgcccacc acacggtcct
     4621 ggaacagcca gcgcgttccg tcgccgatac cctgggtgca gatggccgtg aagcagcgca
     4681 ccacgtcgtc ggccgtgagt gtccgcccta tcccgtccgt aaaggcgggg tcgtcgtgga
     4741 aacgtacgcc ctgccgaagg tggaaggtcc agatcgtgtg cgtggtgtcc acctcccagc
     4801 tatcggccaa gcagggctcc gtggccatgg tggcggggtc gaacgacacg agcccttcgt
     4861 acacctggcc catgatgcgc acttcggacg cctgggtgat cgtgggcgga aagatgctgc
     4921 ggatggcacc ggacacattg aggttgaaga cacccccata acggccttcc gcacttaccg
     4981 tgcgttgtgg tcgatcgtcg caggcactga gcaggacgag gagggagaga acgagaggac
     5041 cggcccgcat ggtggctgcc caaaagtaga agggcgcact tcgggcaacg gtgcgggatt
     5101 tccggcgtta ccaacatgtc cgtcttgatg gacgccgcac cttcgcgagc catcccccac
     5161 cagggtggca accgttactt tccaaccgct tccgccgcct tgccctccgt gtccagcccg
     5221 atgcactaca cctggcgcta tccggcggcg gttttcctcg tgctggcgat gaccggcccc
     5281 gcggtggcgc aagaactgga taaccggcgc gaacggcgca tcgtgctcga tcgcgatacc
     5341 gtacggctcg actccatgag catcgctccc gggtcactca cgttgtggat ggacagcgtc
     5401 gcgctggaca ccgcgttcta ccggaacgac ccctggcacg gcctgatcct ccgccgaccg
     5461 ggagcaccca tcgatacgct catcgcgcgt tatcgcgtgt tgccgctcct gctccccgga
     5521 cccttccacc acaaggacat ccgcctcagg accgctgcca tggccgatcg tacggatccc
     5581 ttcaaatacg tacccggcga gggcaaggag gatccgttgg gcatccatgg ccttaccaag
     5641 agcggcagta tttcccgggg tgtgctgttc ggcaacaacc aggatctgag cgtgaacagt
     5701 gcgctcaatc tcgaattgag cggcaagctg accgagcgaa tcggcgtgct ggcatccatc
     5761 accgacaaca atattcccat tcaggccggg ggcaacacgg cggaattgca ggacttcgac
     5821 cgcgtgttca tcaaactctt cgacgagcgg caagaactga tcgcgggcga cttcgtgctg
     5881 gaacggccca agagccactt cctcacctac tacaagaagt cgaaagggct cggctattcc
     5941 accctgctgg gcaaggaccc gttggacacg ctgagtcgga aagcccgcgg aacgttcgcg
     6001 gtgagtgccg cgatcagcaa gggaaaattc gcgcgcaatg tcatccaggg cgtcgaaggc
     6061 gtgcagggcc cgtaccgcct cagcgccacg gatggtggag cgttcatcat cgtgctcagc
     6121 ggcaccgaac gggtattcat caacggacaa ctcatggccc ggggtcagga gaatgattat
     6181 gtgatcgatt acaacaccgc tgaactgaca ttcacggcca ggcgcctcat caccaaggac
     6241 aggcgcatca cggtggagtt ccagtacagc gacaagaact acgcccgttc gcaggtgcgc
     6301 ttctccaact cgtggtcgat cggacgtacg gacctgtacg ccgatctcta caccgaacag
     6361 gaccacaaga accaaccact gcagcagacc ctgagcgatg ccgaccggct ggtgctctcc
     6421 caggccggcg acgatccatt ggccgcggcc gcacccggca tcgacagtac gggatggaac
     6481 ccggacgaag tgctttacct ccgtcgcgat tcactcgggt atttccccgt gttcgttcac
     6541 agcaccgact ccgtgaatgc ccgattcagg gtatcgttca gcaacgtcgg gccgggtaac
     6601 ggggattacg tgcaggaaga attcacgccc
//
LOCUS       DAOQOC010000016         6585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4496_length_6585_cov_3.675957, whole genome shotgun sequence.
ACCESSION   DAOQOC010000016 DAOQOC010000000
VERSION     DAOQOC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4496_length_6585_cov_3.675957"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2753
                     /locus_tag="PL106_00510"
     CDS             <1..2753
                     /locus_tag="PL106_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA31573.1"
                     /translation="DFDDGTVDSTSGAMVDHTFTQPGEHVVQLYLTDDNECGNLNLID
                     QQVLVSTTPNFGNTSESLETCLGSTVSIYGNAQPVTWTGIPDANFGDGVFLPDDVGTP
                     FSSSLNFQQFDPGQTVTDVSNIQSICVDMEHTYMGDLVLQVICPNGQTMIFHQQGGGY
                     TYLGAPNDFDTNIDPVVGECWHYCWSPTATDGTWVDNSVFGTNNTTSAGTPPSQSLNP
                     GTYEPVQPFSNLIGCPLNGEWTYQSTDLWGADNGFICSWEINFDPAIIPDVTTFTPSI
                     GPEADSSQWDGGTTPDYVSADGDTAVFTATTEGVHPFVYTVTDNFGCTYDTTITVTVF
                     PAFTANAGPDQTICNDPVQLSASVQNSSGQSMTYTWSPPEGLSNPNIANPSALPDNTT
                     TYTLTAFVTGHPECSDTDDIIVALDPGVDPGQDTTISVCMYPPSFDMITMLGGTPNAG
                     GTWTLAGQPADPIFDPTSSPAGTYTYTVTNNLGCTGSADLTIILLDATDPLCCGVVDA
                     GPDTILCELDHGLHASIGNTGTGGWSGPAGYTFGNSGAPQTTVTAPGSGAATFYWIED
                     DGVLCYLIDSVTIIFTEPLQATVTPVDAVCFEACDGTASVAVTGGNGAFTYVWSGGSG
                     GNNPLTEHLCAGDYIVAINDTNMCATSANLTIGQPPLLEIDNNSQVEPWCNGDCNGSL
                     TIIDPEAVDYSFDGGVSWQSNATLDSLCAGSFNIAIRNADGCIGTAQATVTEPPAVTA
                     DFAHLPIPANINAPTIDFHDQSEHAVAWVWDIAGLASSTDQNPRYTFSDKYPGVYEVC
                     LVAIDNHGCRDSICHEVVIDNVLQTYIPNAFTPDGNGVNDSWWMVRNMDDVTDFHMRV
                     FDRWGEVVFESTDPNIAWDGTFRNGGGEILKQDVYAYRCTFQLISTAGAREYMGHVSL
                     LK"
     gene            2807..6130
                     /locus_tag="PL106_00515"
     CDS             2807..6130
                     /locus_tag="PL106_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31574.1"
                     /translation="MPNSRILRLVVAILACWTTAGHAQNIFNIANGDVVTCTGAMVDS
                     GGEGGPGYGNNEQFTATICPDQPGDAISLNTVIFNLNAGGTAPGDELSIYDGASTNDP
                     LIGTWTSGDSPGIIPASFGNPTGCLTISFTSNEIGTGAFAFAITCFVPCEPPVAVAVM
                     SEPSPALICQGEAVLFDGSGSTAPAGFNVTQWIWDFGNGVIDSTSGATVSYVFPDPPG
                     GHVVHLIVEDDNGCVNTNSVELEVRVSTTPTFTSMDDQTLCVGESALLDADAQPVTWS
                     GQPVVDFGDGLALPDEQGVVFTSTITMSAFGVGQTMIDVNDILSVCFDMEHSFIGDFV
                     LQMTSPTGQTIIFHQQGGGGTYLGIPVDQDNMPNAIGTCWTYCFSPTATNGTWVDNAG
                     GTLPAGTYESLQPFSNLLGSQLNGTWTISFTDLWGLDNGFICSWWIEFDPALLPDVTA
                     YTPVLDLSDPDSSGWSGAVLTTDPQDPTHATFTPVSAGPQTLTFSVTDNFGCTYDTTL
                     TVNVNPAANVEATATPPEECGDPWQLNAALQQPIPQGPVTYYWTPTTGLNNSISPYPF
                     ASPDQDTWYVITAFPSGHPLCGTTDSVLVNGLTWLENDSIVTDAICHGDGTGHIQVVT
                     TGNGGPWNYSWMNAGGTVVQTTMGANGDNFSGAGGTWTVVVSEGANGNNCSDTLQAVI
                     LEPSAVEMVSLSDDTLICRTGTATLVADVTGGTGSHTVHWSDGATGATHAVGPLGTTT
                     WEVWATDSSGCMSDTLEVEVAVCPPMGLSVPDTVVTCFEQTTLLMPDTVFGGDGAYQY
                     AWAGGTFTPDPGYTAAWQDTTLVCLTVTDGCETPDVTRCVLVAVKQIPDLVLTADTTE
                     GCDPFFVIFNVQDTTGGATVTWNFGDGTVVNGPPAPVGHTYMDPGVFDVKVTAHWPNG
                     CDDDSTFSDMIIVAAVPDAQFTWSPNPASTLDPTVHFAEQAGPYAVMWSWDLAGLDTA
                     MSPDPVFVFPNDVGGHYPVQLVVANYLGCTDTIVRTIEVMDEFLVFVPTAFTPDGDGV
                     NEQLFVLGDDIDTQAFVLNIFNRWGQMVFSTEDRSIGWDGTFGGKPVPDGVYNWKLEA
                     RSLWTGISHKLSGHVSVLR"
BASE COUNT         1339 a         2099 c         1923 g         1224 t
ORIGIN      
        1 gggacttcga tgatgggacc gtggacagca cgagcggcgc gatggtggac cacacattca
       61 cccaaccggg tgagcacgtg gtgcaattgt acctgaccga cgataacgaa tgcggcaacc
      121 tcaacctcat tgaccaacag gtgctggtga gcaccacccc gaacttcggg aacacttcgg
      181 agagcctgga aacctgcttg gggtccacgg tttccatcta tgggaacgca caaccggtca
      241 cctggacggg catacccgat gccaacttcg gggacggggt gttcctgccg gacgatgtgg
      301 gcacaccttt ctcgtcatcg ctgaatttcc agcaatttga tcccggccaa acggtaactg
      361 atgtctcgaa catccaatcc atctgcgtgg atatggagca tacgtacatg ggcgatctgg
      421 tgctgcaggt gatctgcccc aatggacaga ccatgatctt ccatcagcag ggcggtggct
      481 acacctatct cggtgcaccc aatgacttcg acaccaacat cgatcccgtg gtgggcgaat
      541 gctggcacta ctgctggagc cccaccgcga ccgatggtac ctgggtggac aactcggtgt
      601 tcggcacgaa caacaccaca tcggccggca ccccgcccag ccaatcgttg aacccgggca
      661 cgtatgagcc cgtacagccg ttcagcaacc tgatcggatg cccgctcaat ggcgaatgga
      721 cctatcagag cacggacctc tggggtgctg ataacggatt catctgctct tgggagatca
      781 acttcgaccc ggccatcatt ccggacgtca ccacgttcac gccgagcatc ggtccggaag
      841 ccgattcgtc ccaatgggac ggtggcacca caccggacta cgtatcggct gatggcgaca
      901 ccgccgtgtt cacggccacc acggaagggg ttcacccgtt cgtgtacacg gtgaccgaca
      961 acttcggctg cacctacgac accacgatca ccgtaacggt attcccggcc ttcaccgcca
     1021 acgcggggcc cgaccagacc atttgcaacg acccggtcca actgagcgcc agcgtgcaga
     1081 acagttcggg gcagtccatg acctacacct ggtcgccgcc ggagggcctg tcaaatccca
     1141 acatcgccaa tccgagcgcg cttcccgaca acaccaccac atacaccctc accgcattcg
     1201 tgacggggca ccccgaatgc tccgataccg acgacatcat cgtcgcattg gaccccggcg
     1261 tggacccggg gcaggatacc accatcagtg tatgtatgta cccaccctca ttcgacatga
     1321 tcaccatgct gggtggaaca ccgaacgcgg gcggtacatg gaccctcgca gggcaacccg
     1381 ccgacccgat attcgatccc acgagcagtc cggcgggcac ctatacctac accgtgacga
     1441 acaacctggg ctgcaccggc tcggccgacc tgacgatcat cctgctggac gccaccgatc
     1501 ccctgtgctg cggtgtggtg gacgcggggc cggacaccat cctgtgcgaa cttgatcacg
     1561 gactccacgc ttccatcggc aacaccggaa cgggcggttg gagcggaccg gccggatata
     1621 ccttcgggaa ctccggagct ccgcaaacca cggtaaccgc cccaggcagc ggtgcggcca
     1681 cgttctactg gatcgaggac gacggtgtgc tctgctacct gatcgacagc gtgaccatca
     1741 tcttcacgga acccctgcaa gccacggtga cacctgtgga tgccgtgtgt ttcgaggcct
     1801 gcgatggcac ggccagtgtt gctgtaaccg gtggaaatgg agcattcacc tacgtgtgga
     1861 gcggcggcag cggtggtaac aacccgctga ccgagcattt gtgcgcgggt gattacatcg
     1921 tggccatcaa cgacacgaac atgtgcgcca cttcggcgaa cctgacgatc ggccagcccc
     1981 cgttgttgga gatcgacaac aacagccagg tggagccatg gtgcaacggt gattgcaacg
     2041 gcagcctcac gatcatcgat cccgaggcgg ttgactacag ctttgacgga ggggtcagtt
     2101 ggcagagcaa tgccacgttg gacagtttgt gcgcgggcag tttcaacatc gccatccgga
     2161 atgccgacgg ctgcatcggt acggcccaag ccacagtgac cgagccgccc gcggtcacgg
     2221 ccgacttcgc ccacctgccc atacccgcca acatcaatgc ccccacgatc gatttccacg
     2281 accagagcga gcatgccgtc gcgtgggtat gggatatcgc cgggctggcc tcctccaccg
     2341 accagaaccc ccgatacacg ttcagtgaca aatacccggg ggtgtacgaa gtgtgccttg
     2401 tggccatcga caaccatggc tgtcgcgact cgatctgcca cgaggtggtc attgataatg
     2461 tactccagac gtatatcccc aacgcattca cccccgacgg gaacggcgtg aatgactcgt
     2521 ggtggatggt gcgcaacatg gatgacgtga ccgacttcca catgcgggtt ttcgaccggt
     2581 ggggtgaggt ggtcttcgag tcgaccgatc cgaacatcgc ttgggacggt acgttccgca
     2641 atggcggggg cgagatcctg aaacaggatg tttacgcgta ccgctgcacc ttccagctga
     2701 tcagcacggc gggtgcccgc gagtacatgg gccatgtatc gctgctgaag tgaacgctca
     2761 accggccaca ccgacccctc gggccaagaa gccatcttcg cgccccatgc cgaactcccg
     2821 gatcctccgc ctggtcgtgg ccatcctcgc gtgttggaca accgccgggc acgcccagaa
     2881 catcttcaac atcgccaatg gcgacgtggt cacctgtacg ggtgcaatgg tcgatagcgg
     2941 aggggaaggt ggacccggtt acgggaacaa cgagcagttc accgccacca tctgtccgga
     3001 ccaacctggg gacgctatct ccctgaacac ggtgatcttc aacctgaacg ccggtggtac
     3061 cgcaccgggc gacgagttga gtatctacga tggggcctcc accaacgatc ccctcatcgg
     3121 gacgtggacc agtggtgatt caccagggat cattcccgcc agcttcggca atcccaccgg
     3181 ctgcctcacg atcagcttca cctccaacga gatcggcacc ggggcgttcg ccttcgcgat
     3241 cacctgtttc gttccctgtg aaccgcccgt ggcggtggcg gtcatgagcg aaccttcccc
     3301 cgcactcatc tgccagggtg aggccgtgct gttcgatggc agtgggtcca ccgcaccggc
     3361 gggcttcaat gtgacacagt ggatctggga cttcggcaac ggtgtcatcg acagtaccag
     3421 cggtgcgacc gtgtcctatg tattccccga tcctccgggc gggcatgtcg tccacttgat
     3481 cgtggaggac gacaatggat gcgtgaacac caacagcgtg gaattggagg tgcgcgtgag
     3541 caccacgccc accttcacca gcatggacga tcaaacgcta tgcgtgggtg aatcggcgct
     3601 attggacgcg gatgcacagc ccgtgacctg gagcgggcag cccgtggtcg acttcgggga
     3661 tggactggcc ttgccggatg aacaaggtgt ggtcttcacc agtaccatca ccatgagtgc
     3721 gttcggggtc gggcagacca tgatcgacgt gaacgacatc ctgtcggtct gcttcgatat
     3781 ggagcactcc ttcatcggtg attttgtcct gcagatgacc agtcccacag gtcagacgat
     3841 catcttccac caacagggcg gagggggcac ctatctaggc ataccggtgg accaggacaa
     3901 catgcccaac gccatcggca cctgctggac gtactgcttc agtccaaccg ccaccaatgg
     3961 cacatgggtc gataatgcgg gaggcacgct gcccgcaggg acgtatgaaa gcctgcaacc
     4021 cttcagcaac ctgttgggtt cgcagctcaa cggcacgtgg accatctcct tcaccgacct
     4081 gtggggcttg gacaacgggt tcatctgctc gtggtggatc gagttcgatc cggcactcct
     4141 gccggatgtg acggcctata ccccggtact cgacctcagt gatcctgatt cttcgggatg
     4201 gagcggcgcc gtattgacaa cggaccctca ggatcccaca cacgccacct tcacccccgt
     4261 atcggccggt ccgcaaacgc tcaccttcag cgtgacggac aacttcggtt gcacgtacga
     4321 caccacactc accgtgaacg tgaatccggc cgccaacgtg gaggccacgg ccacaccccc
     4381 agaggagtgc ggcgacccct ggcaactgaa cgccgcgctc caacaaccca tcccacaggg
     4441 cccggtaact tactactgga caccgaccac agggctcaac aacagcatca gtccataccc
     4501 gttcgcctca cccgaccagg acacctggta tgtgatcaca gccttccctt ccggacatcc
     4561 gctgtgcggg accaccgata gtgtcctggt caatggcctc acctggctgg agaacgacag
     4621 catcgtaacg gatgccatct gccatgggga tggcacgggc catatccagg tagtgaccac
     4681 cgggaacggt gggccttgga actattcctg gatgaacgca ggcggaaccg tggtacagac
     4741 cacaatgggt gccaatggcg acaacttcag tggcgcagga ggcacatgga cggtggtggt
     4801 gagcgaaggc gccaacggca acaactgctc ggacacattg caggccgtga tcctggagcc
     4861 ctccgctgtg gaaatggtct ccctgagcga tgacaccttg atctgccgca ccggcacggc
     4921 cacactggtg gccgatgtta ccggaggtac cggttcgcac accgtccatt ggagcgacgg
     4981 agccaccggg gcgacccacg ctgtgggacc actcggcaca accacctggg aggtgtgggc
     5041 aacggacagc agcggttgca tgagcgatac gctggaggtt gaagtcgccg tttgtccgcc
     5101 aatggggctt tcagttccgg acaccgttgt cacctgtttc gaacaaacca cgctccttat
     5161 gccagacacc gtgttcggtg gcgacggcgc ataccaatac gcctgggcgg gcgggacgtt
     5221 cacgccggat ccggggtata ccgcggcatg gcaggacacc acgctggtct gtctgaccgt
     5281 gaccgacggg tgcgaaacac ctgatgtcac ccgctgcgta ctggtggccg tgaaacagat
     5341 accggatctg gtattgacag ccgataccac cgagggttgt gacccgttct tcgtcatctt
     5401 caacgtgcag gacaccacgg gcggcgccac ggtgacctgg aacttcggcg acggcaccgt
     5461 ggtgaacgga cctcccgcac cagtgggaca tacgtacatg gatccaggtg tttttgatgt
     5521 gaaggtgacg gcccattggc ccaatggttg cgatgacgac agcacgttct cggacatgat
     5581 catcgttgcg gccgttccag acgcgcaatt cacctggtcg ccgaaccccg ccagtaccct
     5641 cgacccgacg gtccacttcg ccgaacaggc cggaccgtac gccgtgatgt ggtcatggga
     5701 tctcgcaggc ttggatacgg cgatgagccc ggaccccgtg ttcgtgttcc ccaacgatgt
     5761 gggcggccac taccccgtgc aattggtggt ggccaactac ctgggttgta cggacacgat
     5821 cgtccgcacc atcgaggtga tggatgagtt cctggtcttc gtccccaccg cgttcacccc
     5881 cgacggggac ggcgtgaatg agcaactttt cgtactcggc gacgacatcg acacacaggc
     5941 ctttgtgctg aacatcttca accgctgggg tcagatggtc ttttccaccg aggatcgttc
     6001 catcggctgg gacgggacgt ttggagggaa gccggttccc gatggcgtct acaactggaa
     6061 gctggaggct cgctcactct ggacggggat ctcgcacaag ttgtcgggcc atgtgagcgt
     6121 gctgcgctga tgtgcggccc catcgagcgg tcgacttttc acgtcgacat tgttgatcgt
     6181 tccgtcggga atcctccagt agcgatccgg gccggatagc ttcacatcgg ctgtccgcac
     6241 cactgaccgg cctcgatcgc atggtttgat cttacgcgac ccaagatcag gtatctataa
     6301 acgtcgaccg accagagcgg tcatcggtcg ctccatgcct gccagggcca tcggccttct
     6361 cgtgccgctg ctcgccaccc tttcccacgc acaggttttg ggatcggcaa cgcccaggtg
     6421 agcacgtgcg ccggtgcatt cctggacagt ggcggtcagg gtgcttccgg ttactcgaac
     6481 aacgaggact acacgtacac gatctgcccc gatgcacccg gggcggccat ttcggtgaac
     6541 ttcgtcaccg cacacgatgg cttcacgctg cgcgacttgg tctcc
//
LOCUS       DAOQOC010000017         6539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4547_length_6539_cov_3.408698, whole genome shotgun sequence.
ACCESSION   DAOQOC010000017 DAOQOC010000000
VERSION     DAOQOC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4547_length_6539_cov_3.408698"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PL106_00520"
     CDS             complement(<1..388)
                     /locus_tag="PL106_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31575.1"
                     /translation="MDLYSRKQRWKLALAMVALAIVGAGLWYSGRMVERVREEERHKV
                     TLWAEAVRNRAQLVNYTDSLFRTLRDEERKKVELWADASARLVSSAPGEHLGFYLKVA
                     QNNTTIPVIITDGLGHVRSSRNIPVRI"
     gene            complement(424..1146)
                     /locus_tag="PL106_00525"
     CDS             complement(424..1146)
                     /locus_tag="PL106_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31576.1"
                     /translation="MPSKHTGGLVAVLFVLLTTGATAQKNKPRTKVVLYKDRIAAQWD
                     TVNCVKNAIKINPLLFLRGEIPVYYERALSAKLSAELGVGVTYRNYINLTIAGDHQDA
                     DDFGAGTRILSHPSFHVGLRYYFTDDLEPQGAYMQAEFAYLKYGKLITEQDSTGQLSD
                     RRRTDQRTFNDFRLYFGYQTLGASSNWLFDFYGGVALRLRDMTIVNEKLNVAARQWTY
                     DEERINDMVPAIFLGMKMGLGW"
     gene            1279..2211
                     /locus_tag="PL106_00530"
     CDS             1279..2211
                     /locus_tag="PL106_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNA31577.1"
                     /translation="MRAFETRFREAMRSKVPLLDRIMHYIVQRKGKQMRPMFTLLSAK
                     QFGPITDAGYTAASLIELLHTATLVHDDVVDGSPVRRGFFSVNALWKNKVAVLVGDYL
                     LSRGLLLAVEKGEFQLLRLVSRAVREMSEGELLQMEKARGLNFSEDVYFEIIRQKTAS
                     LIAACCASGAAAGGAAPEEVERMHTFGELAGIAFQIKDDLFDYGAGKNTGKPTGIDIK
                     EKKLTLPLIFTLAHADPSDRRWMVRTVKHDNSNSAAVAKVIAKVEECGGIAYATKAMY
                     NYRDRALDVLRALPENDARRALEGLLNLTVERVK"
     gene            2208..2666
                     /locus_tag="PL106_00535"
     CDS             2208..2666
                     /locus_tag="PL106_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31578.1"
                     /translation="MKAPAHILAVLLFTACAGSGTSERPIVQDTAGTAANNENLRRLA
                     VEDSLRDGYHMYKDRDGRPLMEGELLNGKRIGVWTSYLPSGRVHSRNVYDQGVLHGIT
                     TVFHPNGVLYFTGTMRKGRSVGEWKFYDVEGRMARTVTYDTTGAIIGGSK"
     gene            2834..4783
                     /gene="dnaG"
                     /locus_tag="PL106_00540"
     CDS             2834..4783
                     /gene="dnaG"
                     /locus_tag="PL106_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNA31579.1"
                     /translation="MISPRSVQQVKDVVRIEEVLGEFLDLKRKGPRYLGLCPFHNEKT
                     PSFNVSPSLGIYKCFGCGESGDAITFLQKQEHLSFAESIRWLAKRYNIELEEEEQSPE
                     QQLEQSERESLAVVQQWAVDWSVDQLWNTEEGRTIGLAYFRERGVRDEVIRQFQLGYV
                     PEQGAAFAKAALAHGFTMDALEKAGWIKLREDGAAWDFFQGRVTFPIHGLTGQPIAFG
                     ARTLRSDKKLPKYFNSPESVLYNKSRSLYGIAFARKAIADKGSCCLVEGYMDVISLHQ
                     AGITNAVASSGTSLTQDQVRLIKRYATAVTILYDGDPAGIKASMRGIDIILAEGLSAK
                     VVLFPEGEDPDSFARTRSSGEVEAFIAANARDFLVFKADMLMRETGGDPVRKAAAIHE
                     LVESLALIGDHVLRSLYVKQCAAMLELAEQALVSELNKVLRRNHRKKLGGNAPLVEEV
                     LPPAIAPPQPEVEDPGTRPQERDLMRILLNYGHEKVLVPLQYEDGSVVDAELTVGQFM
                     FDELAKDDLGFDDQLLHAIYLDYRKRCNLGEQVTGALYANHADEAWRSVSIDLLTEKH
                     VLSPNWRTRHRIHVAREHDQLLDSLLEGLDILKERRVDRMIGELMQQLKDCAEDDMPI
                     ILDRIRKLTAVKMDFSKRTGRVVVG"
     gene            complement(4874..6283)
                     /locus_tag="PL106_00545"
     CDS             complement(4874..6283)
                     /locus_tag="PL106_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31580.1"
                     /translation="MRHISTAILLVALSTGAWAQGINSVVITPQNPTTCTLIHIQFNG
                     SMPQQANLTNFTSTISGDTMYVTFFASGNGGQNTSFTQGIDLMAPSAGTWVIQTGFVL
                     NGNQVGLNTQTINVAVGVTSNPGEYGELTGLCTGGTAIPLISILGGTPDPGGSWTDPL
                     GQVVPNGQFVPGQSPAGFYIYNFDVNPPCNDTAQYIYVDYVTPSAIPGLNATVQVCEN
                     WGGTVNLFDTLGGTPDTGGTWTAPGGAPHSAVYDPLTDPCGAYTYTVPGIAPCPAATA
                     TITIQCVSQPNAGSVSAANDTINRCYNDTSEVLSQFVTGEQNTGTWYNGAGFAAGPYN
                     AHINIAANGPGTYVYVVPGGVCPNDSTFIHVVLWGDSIVGCTVGMDEPVDGFSRFELM
                     PNPAQDQVTIELERDRDTGPAWIEIVGVDGKLLRRASLTFNGLFARTTLGVGDLPRGA
                     CIVRVMGDQGALARRLMLR"
     gene            6357..>6539
                     /locus_tag="PL106_00550"
     CDS             6357..>6539
                     /locus_tag="PL106_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31581.1"
                     /translation="MRGLLFTVVWFLAVAGVRAQGPLINEVVPGGGALPDWIEVYNPG
                     PKAVELQGFTLVSTGRT"
BASE COUNT         1243 a         1996 c         2067 g         1233 t
ORIGIN      
        1 cgatgcgtac ggggatgttg cggctgctgc gcacgtggcc cagcccatcg gtaatgatga
       61 ccggtatggt ggtgttgttc tgcgccacct tcaggtagaa acccaggtgc tctccgggag
      121 cactgctcac cagccgcgcc gatgcatcgg cccagagttc caccttcttg cgctcctcat
      181 cgcgcaatgt ccggaacagt gaatcggtat agttcaccaa ttgcgcgcgg ttcctcacgg
      241 cctcggccca cagggtcacc ttgtggcgtt cctcctcccg cacgcgctcc accatgcggc
      301 cgctgtacca gaggccggcg cccacgatgg ccagtgccac catcgccagt gccaacttcc
      361 accgttgttt gcgcgagtag aggtccatgt tcggcgttga cgacggggcc gcggggggtc
      421 ttcctaccag ccgagcccca ttttcatccc gaggaagatc gcgggcacca tgtcgttgat
      481 cctctcctcg tcgtaggtcc actgccgtgc cgccacgttg agcttctcgt tcacgatggt
      541 catgtcccgc agtcgcagcg ccaccccgcc gtaaaagtcg aacagccagt tgctggacgc
      601 tcccaatgtc tgatacccga aatacagccg gaagtcgttg aacgtccgct ggtccgtccg
      661 tcggcggtcg ctgagttggc cggtgctgtc ctgttccgtt atgagcttcc cgtatttcag
      721 gtaggcgaat tccgcctgca tgtacgcccc ctgtggctca aggtcatccg tgaaatagta
      781 ccgtaacccc acgtggaagc tcgggtggct gaggatcctc gtgcccgcac cgaaatcatc
      841 ggcgtcctga tggtcgcccg cgatggtgag gttgatgtag ttgcggtagg tgactcccac
      901 acccagttcg gcactcagct tggcggacag tgcgcgctcg taatagacgg ggatctcccc
      961 gcgcaggaag agcaacgggt tgatcttgat cgcgttcttc acgcaattca ccgtgtccca
     1021 ctgggcagcg atccgatcct tgtacaggac caccttggtc ctgggtttgt tcttctgggc
     1081 cgtggccccg gtggtcagca ggacgaaaag gaccgccacg aggcccccgg tgtgtttgga
     1141 tggcatgccc tgcgaaggta gcggacgatg gcatgcggag cgaggatgcg ggtcagccgc
     1201 tatctgcacg gcctcagtag tttcggcccg ttcgcattgc gtaccctgga cgagatacaa
     1261 gctccgatcg caccggagat gcgtgctttc gaaacgcgct tccgcgaggc catgcgcagc
     1321 aaagtgcccc tgctggaccg catcatgcac tacatcgtgc agcgcaaagg caagcagatg
     1381 cggcccatgt tcaccctgct cagcgccaaa cagttcggac cgatcaccga cgcgggctac
     1441 acggcggcca gtttgatcga actgctccat acggccacgc tcgttcacga tgatgtggtg
     1501 gatggctccc ccgtgcgtcg tggcttcttc agtgtgaacg ccttgtggaa gaacaaggtc
     1561 gccgtactgg tcggcgacta tctgctgagc cggggcctgt tgttggccgt ggagaagggc
     1621 gagttccaat tgcttcgcct ggtgagccgg gccgttcgcg aaatgagcga gggcgaattg
     1681 ttgcagatgg agaaggcgcg cggcttgaat ttctcggaag acgtgtactt cgagatcatc
     1741 cgtcagaaga cggccagcct catcgccgct tgttgtgcca gcggtgccgc tgctggtgga
     1801 gccgcaccgg aggaagtgga acgtatgcac acctttggcg agctcgcggg catcgccttc
     1861 cagatcaagg acgacctctt cgattacggg gcaggcaaga acacgggcaa gccgaccggt
     1921 atcgacatca aggagaagaa attgacgctg cccctcattt tcaccctggc ccatgccgac
     1981 ccgtccgacc gtcgctggat ggtgcggaca gtgaagcacg acaacagcaa cagcgccgcc
     2041 gtggccaagg tgatcgccaa ggtggaggag tgcgggggca tcgcctacgc caccaaagcc
     2101 atgtacaatt atcgtgaccg ggcactggat gtgctgcgcg ctcttccgga gaacgatgcc
     2161 cggcgtgcgc tggagggcct gctgaacctt accgtggagc gtgtgaaatg aaagccccgg
     2221 cgcacatcct cgccgtcctg ttgttcactg catgcgcggg cagcggcacc tcggagcggc
     2281 cgatcgtcca ggatacggcg gggaccgccg cgaacaacga gaacctccgg cgtctggccg
     2341 ttgaggacag cctgcgcgat ggctaccaca tgtacaagga tcgcgatggc cgaccgctga
     2401 tggaaggtga actcttgaac gggaagcgca tcggtgtatg gaccagctac ctgcccagtg
     2461 gccgtgtaca cagccgcaat gtgtacgacc agggcgtcct ccatggcatc accacggtgt
     2521 tccatccgaa tggcgtgctc tatttcaccg ggaccatgcg caagggccga tcggtcgggg
     2581 agtggaagtt ctatgatgtc gagggccgga tggcccggac ggtcacgtac gacaccaccg
     2641 gcgcgatcat cggcggatcg aagtagcgtc cctgttcgag gtgttctgga cgaggaggat
     2701 ccgcctgcgt cacgatccac gtcaacaatt cccggttgag atccatgggg atcgtggata
     2761 accgcaatgt ggacatctca cgccgacccc tgcccgggcc ctcgcgtaca ttcgtgaccc
     2821 tttttccgga accgtgatca gcccccgctc cgttcagcag gtgaaggatg tggtccgcat
     2881 cgaagaggtg ctgggcgaat tcctggacct caagcgcaaa gggccgcgct acctcggact
     2941 gtgcccgttc cacaacgaga agacgccgtc cttcaatgtg agcccatcgc tgggcatcta
     3001 caagtgcttc ggttgcggtg agagcggtga tgccatcacc tttctgcaga aacaggagca
     3061 tctctccttc gctgagtcca tccgctggct cgccaagcgg tacaatatcg aactggagga
     3121 ggaggaacag tcgcccgaac aacagttgga acagagcgag cgcgagagcc ttgccgttgt
     3181 gcagcagtgg gccgtggact ggagcgtgga ccaactctgg aacacggagg aaggccgcac
     3241 catcggtctg gcctatttcc gcgagcgtgg tgtgcgcgac gaggtcatcc gccagttcca
     3301 gctcggctac gttccggaac agggcgccgc cttcgccaag gccgccctgg cccacggctt
     3361 caccatggat gcgctggaga aggccggttg gatcaagctg cgcgaggacg gcgctgcctg
     3421 ggattttttc cagggccgcg tcacttttcc catccacgga ctcaccggcc agcccatcgc
     3481 gttcggggct cgcacgttga ggagtgacaa gaagctcccc aagtacttca acagccccga
     3541 gagcgtcctc tacaacaaga gccgctcgct gtacggtatc gcattcgcgc gcaaggccat
     3601 cgccgataag gggtcctgct gcctggtgga gggctacatg gatgtgatca gcctgcacca
     3661 ggcggggatc accaacgccg tggccagcag cggcacctcg ctcacacagg accaggtgcg
     3721 cctgatcaag cgctacgcga ccgcggtaac gatcctgtac gatggggatc cagccgggat
     3781 caaagcctcg atgcggggca tcgatatcat cctggccgaa ggactcagcg ccaaggtggt
     3841 gctcttcccc gagggggagg accccgacag cttcgcgcgc acccgcagca gcggcgaggt
     3901 ggaggccttc atcgccgcga acgcacgcga cttcctcgtc ttcaaggccg acatgctcat
     3961 gcgcgagacc gggggcgatc ccgtgcgcaa ggccgcggcc atacacgaac tggtggaaag
     4021 cctcgccctc atcggtgacc acgtgctgcg ctcgctctat gtgaagcagt gcgcggccat
     4081 gcttgagctg gccgaacagg ccctggtgag cgagctgaac aaggtgctgc ggcgcaacca
     4141 ccgcaagaag ctcggcggca acgcgccttt ggttgaggag gtcctcccgc ccgcgatcgc
     4201 accaccccag cccgaggtgg aggacccggg aacgcgcccg caggaacgcg atctgatgcg
     4261 catcctgctg aactacggcc atgaaaaagt ccttgttccc ttgcagtacg aggacggcag
     4321 cgtggtggat gccgaactca ccgtgggcca gttcatgttc gacgaactgg ccaaggatga
     4381 cctgggattc gacgaccagc tgctgcatgc catctacctc gactaccgca aacggtgcaa
     4441 cctgggcgaa caggtgacag gtgccctgta cgccaaccat gccgatgagg cctggcgatc
     4501 ggtatccata gacctgctta ccgaaaagca tgtattgagt ccgaattgga ggacacgaca
     4561 ccgcatccat gtcgcacggg agcacgatca attgctcgat tccctgctgg agggcctgga
     4621 tatcctgaaa gaacgccgcg tggaccggat gatcggggag ttgatgcagc agttgaagga
     4681 ttgcgcggag gacgacatgc cgatcatcct ggaccggatc aggaaactga ccgcggtgaa
     4741 gatggatttc tccaaacgca ccggccgggt ggtggtgggg tgagtcgccg cccgtaccgg
     4801 ttgaactgga tcccataaga aagccccggc cccggaacgc gggtcggggc tttcccttgg
     4861 cgaacggccg cgatcagcgc agcatcagcc tgcgggccaa cgcgccttgg tcacccatca
     4921 cccgaacgat gcaggctccg cggggcagat cgcccacacc caaggtggta cgtgcgaaca
     4981 gcccgttgaa ggtcagtgat gcgcggcgca gcaacttgcc gtccacgcca acgatctcga
     5041 tccatgccgg tccggtatcc cggtcgcgtt ccaactcaat ggtcacctgg tcctgtgccg
     5101 ggttgggcat cagttcgaag cggctgaaac catccacagg ctcatccatc ccgacggtac
     5161 aaccgacgat cgaatcgccc cagagcacca catggatgaa ggtggaatcg ttggggcaca
     5221 ctccgccggg caccacgtac acgtatgttc cgggcccgtt cgcggcgatg ttgatatggg
     5281 cattgtacgg acccgccgcg aaaccggcac cgttgtacca ggtgccggtg ttctgctccc
     5341 cggtaacgaa ttgggagagc acttcggacg tgtcgttgta acagcggttg atcgtgtcat
     5401 tggccgctga taccgatccc gcattgggct gtgagacgca ctggatggtg atggtggcgg
     5461 tggcggccgg gcacggcgct ataccaggga ccgtgtacgt atatgcaccg caggggtccg
     5521 tcaacgggtc gtacaccgcc gagtgtggtg ccccgccggg agcggtccag gtacctcccg
     5581 tgtcgggcgt gcctcccagt gtatcgaaca ggttgacggt gccgccccag ttctcacata
     5641 cctgcaccgt ggcgttcaac ccggggatcg cactaggggt gacgtagtcc acgtagatgt
     5701 actgtgccgt gtcattgcat ggggggttca catcgaagtt gtagatgtag aacccggcag
     5761 ggctttgacc gggtacgaac tgcccgttgg gtacgacctg gcccagcgga tcggtccacg
     5821 aaccacccgg gtccggggtg cctccaagga tgctgatcag ggggatcgct gtaccgccgg
     5881 tacacagccc ggtcaattcg ccgtactcac cgggattgct ggtaacaccc accgccacat
     5941 tgatggtctg tgtattcagg cccacctggt tgccattcaa cacgaatccg gtctggatca
     6001 cccaggtgcc agcacttggc gccatcagat cgatgccttg ggtgaatgag gtgttctgac
     6061 ccccgttccc tgaggcgaag aacgtgacgt acatcgtgtc ccccgagatc gtcgaggtga
     6121 agttcgtcag gttggcctgc tgcggcatgc tgccgttgaa ctggatgtgg atcaacgtgc
     6181 aggtggtggg gttctgcggc gtgatgacca cactgttgat gccctgggcc caagcgccag
     6241 tggacagcgc gacgaggagg atcgcggtgg agatgtgtct catgcggaag gtggacttgt
     6301 tcgctttgcg gcgaaagtag ggcgacgggc cgagccgatc gtccaatttt gccccgatgc
     6361 gcgggctctt gttcacagtc gtctggttcc tggccgttgc cggggtgcgc gcccaaggcc
     6421 ccctcatcaa cgaagtggtg cccggaggcg gggcacttcc ggattggatc gaagtgtaca
     6481 atccaggccc caaagccgtg gaacttcaag gcttcacgct ggtgagcacc gggcgtacg
//
LOCUS       DAOQOC010000018         6443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4645_length_6443_cov_2.963056, whole genome shotgun sequence.
ACCESSION   DAOQOC010000018 DAOQOC010000000
VERSION     DAOQOC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_6443_cov_2.963056"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PL106_00555"
     CDS             complement(<1..692)
                     /locus_tag="PL106_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNA31582.1"
                     /translation="MATSIAGPPAHIALLGNPNCGKTALFNLLTGSRQKVANYAGVTV
                     ERKEGVLHTASGRRVRVLDLPGAYSLNALSSDEAITRDVVTGHSRESLPDLLVCVVDA
                     TNLRLHLRLVLEARQLGLPMVVALNKTDRAEKLGIRVDRDQLAKELGVPVVGTVSVQS
                     HGARELLAFLDKPLPLRTTSTWRAPDINTVRHTQEEVHRILSTAVKEPLEDTDPSGRI
                     DRVLMHPVWGMI"
     gene            complement(695..943)
                     /locus_tag="PL106_00560"
     CDS             complement(695..943)
                     /locus_tag="PL106_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNA31583.1"
                     /translation="MRLDLLPGHCWATVTDVMVPVGRRDRELVVRLTELGFVRGERVR
                     IVAEGIGRAREPLAVRVGHSTFALRKHEASFIHVEPDR"
     gene            complement(1059..1664)
                     /locus_tag="PL106_00565"
     CDS             complement(1059..1664)
                     /locus_tag="PL106_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNA31584.1"
                     /translation="MARKPRQDVLTLFDSLVYTCGYRLVIDPASHVAARVLEWRGTLR
                     ERIGKFEEAYQMPGITLLRTELPPEYEGPLADAIERGAQGFAPFALNLGAMAHSEDRG
                     SIHIEIAEKGTIAALRQRILDHVRANRRIRKLGVEVNEQARLTIASGLKSEQFQTAWA
                     MLAPLGFTAQQRVSDVVLMKRELDHTSIDRHVRTFALEAPH"
     gene            complement(1896..2273)
                     /locus_tag="PL106_00570"
     CDS             complement(1896..2273)
                     /locus_tag="PL106_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31585.1"
                     /translation="MPELNDDDLRKLFRTAGHAAPGSDLSARIMARVAVTPMARPLVV
                     EPLIGRRGWIVLAIMAVMLLIATFVIPGTSVPTGPSWFAPMDEVLKHIRLPQDWSLWA
                     LLASTTALLFTLVDRSLAHKSVQ"
     gene            complement(2266..2841)
                     /locus_tag="PL106_00575"
     CDS             complement(2266..2841)
                     /locus_tag="PL106_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA31586.1"
                     /translation="MRTRVNEHEAIARALTGDQHAYERIVRRYQHMVHTVCLRMLRNT
                     QDAEEATQDTFVKAWQHLEGFGGNAKFSTWLYSIAYRTAISHGRKRRDSGPSMDELAH
                     HPPAEHKDETHRTDIKYHLDLALATLHAEDASILSFFYLEELSVEEIVTVTGLGASNV
                     KVKLHRGRKKLLDALNKQLGPEAHALLLDHA"
     gene            3019..3381
                     /locus_tag="PL106_00580"
     CDS             3019..3381
                     /locus_tag="PL106_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31587.1"
                     /translation="MPYSETLVALAFFATTFGIAYLAINARHRQRMAMIEKGVDAAAL
                     LSYTGLKLGYLGVGAGAGLGLGWFVDSIIHGGQNSDNPVAYFICLLVCGGLSLILAHQ
                     RIAKERAKVVAGKQGPGI"
     gene            3416..4159
                     /gene="mtgA"
                     /locus_tag="PL106_00585"
     CDS             3416..4159
                     /gene="mtgA"
                     /locus_tag="PL106_00585"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNA31588.1"
                     /translation="MKHTILRSLRWLAAAVLWSFVVCVLWVLVLRVIPPPVTWVMVDQ
                     SNEQGTLQRDWCALDEMSHSMPLAVIASEDQRFLHHHGFSWTAIKKAMEYNARKKGKK
                     VKGASTISQQTAKNVFCWPGRSFLRKGVEAGFTVLIEALWSKERIIEVYLNVAETGKG
                     RFGVQATALTCFKHDASRLSTAECALIAAVLPLPRKFNACAPSGYMRKRQGDIQRQMR
                     LLGDVFDPAVMERTRERIRKEEEQERKGK"
     gene            complement(4251..5315)
                     /locus_tag="PL106_00590"
     CDS             complement(4251..5315)
                     /locus_tag="PL106_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNA31589.1"
                     /translation="MRNNTWKELLLMNSTDDWFMALLFVLGGWLLGQVVSRVAKGLLG
                     RWAARSSTGTDDMLLRELRSPVVLIITVLGFLFGYQHLNWPARADLWVWRALHVAIAV
                     SFTWLIARLATRLLGEYLHRGQTNAERRGTAIPALTGVVNVLIWGLGLVVALSNAGYD
                     VGALLAGIGIGGLAMAMAAKDTLANIFGGISLFADEPFTAGDRVRIGEHTGRVEAIGL
                     RSTRLRTAEGPVVVIPNHLFTESMVVNLSAERTQRVRLDIGIDRSMPAEQVARALDVL
                     GSIVNGMPDVLEPEHVASLHDLRPDHLGVILIYHVKKHHPIDAARTRINLEVLRRFQA
                     EGIRMAHPTSVQFVMDGPPA"
     gene            5257..6012
                     /locus_tag="PL106_00595"
     CDS             5257..6012
                     /locus_tag="PL106_00595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA31590.1"
                     /translation="MNQSSVLFISSSSFQVLLRMFRSLPVSRLAAPKEMNTMPVGPAP
                     RPLVVVTGAGRGVGFATALALVRTHRAQVIAVSRDVSAWNAVKEEGITPLTADVATEE
                     GRATVVRAVDGRPLAGLVNNAGLLLKRNLGTWTAADLERLHAVNVAAPLLLTQALLPA
                     LRAASSAHVVNIGSMGGFQGSIKFPGLVGYSASKAALACMSECLAEEWKDTTVRCNCL
                     CLGAVDTDMLREAFPGYVAPVSVGTMGGYIAAL"
     gene            complement(5958..>6443)
                     /locus_tag="PL106_00600"
     CDS             complement(5958..>6443)
                     /locus_tag="PL106_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31591.1"
                     /translation="IWGELQPVRFRELSSHDVLEFLTEFMTPVQRETLESETAVDFAY
                     DSPHGRFRVNVYRHSGGLGAAFRAVAGAPPQLETLGLPNVVQQLLAVGKGLVLWLPNG
                     AVICDELEKLAREWEFRDGYQRVRTPHMTRGITYQRSGHLSLYKAAMYPPMVPTETGA
                     T"
BASE COUNT         1096 a         2082 c         2041 g         1224 t
ORIGIN      
        1 atgatcatgc cccacacggg gtgcatgagc acccgatcga tgcggccact cggatccgtg
       61 tcctccaagg gctctttcac cgcagtgctg agtatgcgat ggacctcctc ctgggtgtgg
      121 cgcacggtgt tgatgtccgg tgcccgccac gtggaggtgg tgcgcagtgg caatggcttg
      181 tccaggaacg ccaggagttc gcgcgcgccg tgtgactgta cgctgaccgt gccgaccacg
      241 ggtacgccca gttccttggc caattgatcc cgatccaccc ggatccccag cttttccgcc
      301 cggtcggtct tgttcagtgc gaccaccatg ggcaggccca gttggcgcgc ttccagcacc
      361 aggcgcaagt gcaaccgcag attggtggca tccaccacgc agaccagcag gtccggcagg
      421 gattccctgc tgtgcccggt gaccacgtcc cgcgtgatgg cttcgtcgct gctgagggcg
      481 ttcaagctgt atgcaccggg caggtccagc acgcgcacac gccgccccga agccgtgtgc
      541 agcacgccct ccttgcgctc aaccgttacg cccgcgtagt tggccacctt ttggcggctc
      601 ccggtgagca ggttgaacag ggccgtcttg ccgcagttcg ggttgcccag cagagcgata
      661 tgggcaggag ggcccgcgat gctcgtggcc atccttaccg atcgggttcc acgtggatga
      721 acgaggcctc gtgcttgcgc agcgcgaagg tggaatgacc cacccgcacg gccagcggct
      781 cgcgcgcacg accgatcccc tcggccacga tccggacccg ctcgccgcgt acgaagccca
      841 gttcggtcaa gcgcaccacg agttcccggt ctcttcggcc gacggggacc atcacatcgg
      901 tcaccgtggc ccagcaatgc ccgggcagca ggtccaggcg catggtggat gggttggtca
      961 tttgcggcgg caaatgtctt cgcttccgcg ccctcgacca ataccccgat cgtgcgatgg
     1021 atgaggaaag cgtgatgggt aggcgagccc tgccctggtc aatgcggcgc ctcgagcgcg
     1081 aaggtccgca cgtgccggtc gatgctcgtg tggtccaatt cgcgcttcat caggaccaca
     1141 tcgcttacgc gctgttgtgc ggtgaagccc aacggggcca gcatggccca tgcggtctgg
     1201 aactgttcgc tcttcaggcc cgacgcgatg gtcaaccgcg cctgctcgtt cacctctacg
     1261 cccagcttgc ggatccgccg gttggcccgg acatggtcca ggatgcgctg ccgcagtgcc
     1321 gcgatcgtgc ctttttcggc gatctcgatg tggatggatc cgcggtcctc gctgtgtgcc
     1381 atggccccga ggttcaatgc gaagggggcg aagccttgcg ccccgcgttc gatggcatcg
     1441 gccaggggac cctcgtattc cggtggcaac tcggtgcgga ggagtgtgat ccccggcatt
     1501 tggtaggcct cctcgaattt cccgatgcgc tcgcgcaatg tcccccgcca ttccagcacg
     1561 cgcgcggcca cgtggctcgc cgggtcaatg accagccgat atccgcaggt gtacaccagc
     1621 gaatcgaaca gggtcagcac gtcctgccgg ggtttgcggg ccatgtgttc ccctccggga
     1681 tcggtccgga ggcgatggtg caaaggaact tccatggatc ccggtcgttg atccggtctc
     1741 tcctgtgact ttccacgatc cccggatgat gacacttccc atgtcacggc ctttcggctg
     1801 agtggtccat gtgctgaaga ccttcacccc ggtcgcgttc gatcgggtcg ttggcgtcgg
     1861 atggaagtgg cggaatggct gctgggcgag cgtccctact ggacggactt gtgcgccagc
     1921 gatcgatcga ccagcgtgaa cagcagtgcg gtcgttgaag ccagaagggc ccagaggctc
     1981 cagtcctgag gcaggcggat gtgcttgagc acctcatcca tcggcgcgaa ccacgatgga
     2041 ccggtaggga cacttgttcc cgggatcacg aaggtggcga tgagcaacat caccgccatg
     2101 atcgccagga cgatccaccc gcgcctcccg atcagtggct ccaccaccaa cggacgggcc
     2161 atgggcgtta cggccacccg cgccatgatc cgtgcgctca ggtcgctgcc cggtgccgca
     2221 tgccccgccg tgcggaagag tttgcgcaga tcgtcgtcgt tcaactcagg catgatccaa
     2281 aagtagggcg tgcgcctccg gtccgagttg cttgttcagg gcatccaaca actttttacg
     2341 gccccgatgc aacttcactt tcacattcga cgcgccgagt ccggtaacgg ttacgatctc
     2401 ttccacgctc agttcctcca agtagaaaaa actcaggatg gatgcgtctt ccgcgtgcag
     2461 cgtggccaat gccagatcca ggtggtactt gatgtcggtc cggtgtgtct catccttgtg
     2521 ctccgccggt gggtggtgtg ccagttcatc catcgatggc ccgctgtccc ttcttttccg
     2581 gccgtgcgaa atggccgtgc ggtacgcgat gctgtacaac caggtactga acttggcgtt
     2641 gcccccgaat ccttcgaggt gttgccaggc cttcacgaac gtgtcctgtg tggcttcctc
     2701 ggcgtcctgg gtgttccgca gcatccgtag acaaacggta tgcaccatgt gctggtacct
     2761 gcgcacgatg cgctcgtacg cgtgctgatc accggtcagc gcccgtgcta tggcctcgtg
     2821 ttcgttcacc cgtgtgcgca tgggacgcca atgatccgta cgcggttacg aatgtcgaat
     2881 gctatcgtat ctcatgagcg gctcatacat gagctgtgcc gggccggtgc ggtcccggtc
     2941 caaagcgctg ttcgataccc tttgtaacca gtggtgagat ggccgcgtct catttcccgg
     3001 accacaacac gaagacccat gccctactcg gaaaccctcg tcgccctcgc atttttcgcc
     3061 accaccttcg gtatcgccta tctggccatc aatgcgaggc accgccagcg catggccatg
     3121 atcgagaaag gggttgatgc ggccgcgttg ctctcctaca caggcctgaa gctcggttac
     3181 ctcggggtgg gtgcgggcgc aggtctcggg ctcggctggt tcgtcgacag catcatccac
     3241 ggtggccaga attcggacaa cccggtggcc tacttcatct gcctgctcgt gtgcggtggc
     3301 ctctcgctca tcctggccca ccaacgcatt gcgaaggagc gcgccaaagt ggtcgccggt
     3361 aagcagggtc cgggcatctg atccccgcta ccttcgcacc ggtaactcac aatcggtgaa
     3421 gcacaccatt ctccgttccc tgcgttggct tgccgctgcc gttctctggt ccttcgtggt
     3481 ctgcgtgctc tgggtcctgg tactgcgtgt gatccctcca ccggtcacct gggtcatggt
     3541 cgatcagtcg aacgaacagg gcacgctgca gcgggattgg tgcgcactgg atgaaatgtc
     3601 ccacagtatg cccctcgctg tgatcgcatc cgaggatcaa cgattcctcc atcaccacgg
     3661 tttttcatgg acggccatca agaaggccat ggagtacaat gcccgcaaaa aggggaagaa
     3721 ggtgaagggc gcgagcacca tcagccaaca gacggccaag aacgtcttct gctggcctgg
     3781 ccgcagtttc ctgcgcaagg gtgtagaagc cgggttcacc gtgctcatcg aggcgctgtg
     3841 gagcaaggag cgcatcatcg aggtgtacct caatgtggcc gagaccggca aaggacgttt
     3901 cggggtgcag gccacggcgc tgacctgctt caagcacgat gcgtcccggc tctcgacggc
     3961 cgaatgcgca ctgatcgcgg cggtactgcc attgccccgg aagttcaatg cgtgcgcgcc
     4021 ctcgggctac atgcgcaagc gacaggggga catccagcgc cagatgcggc tgctgggcga
     4081 tgtgttcgat ccagccgtta tggaacgcac gcgcgagcgc atccgcaagg aggaggaaca
     4141 ggagcgcaag gggaagtgat ggccctgtgg cccggacccc aaaaacaata cccgaaaaaa
     4201 agtcctaccg gtccggcccg ggctcccggt ggatgaaccc ggatggttct tcaggcgggc
     4261 ggaccgtcca tcacgaattg tactgaagtg ggatgggcca ttcggatgcc ctccgcttgg
     4321 aagcgccgca gcacctccag gttgatacgg gtgcgtgccg catcgatcgg atgatgtttc
     4381 ttgacgtgat agatgaggat cacccccagg tggtccggcc gcaagtcatg gaggctcgcc
     4441 acatgctcag gttccaacac atcgggcatc ccgttcacga tgctccccag cacgtccaat
     4501 gcgcgggcca cttgttcagc cggcatgctc cggtcgatac cgatatccag ccgcacacgc
     4561 tgggtgcgtt cggccgacag gttcacgacc atgctttccg tgaagaggtg gttggggatc
     4621 acgaccacgg ggccttccgc cgtgcgcagt cgcgtgctcc gcaggccgat ggcttccacc
     4681 cggcccgtgt gttcgccgat ccgtacgcgg tccccggcgg tgaatggctc atcggcgaac
     4741 aaggagatgc ctccgaaaat gttcgccagg gtgtccttcg ccgccatggc catggccagg
     4801 ccaccgatgc cgatgcccgc cagcagcgcc cccacgtcgt agcccgcatt gctcagggcc
     4861 accaccagac ccaagcccca gatcaacacg ttcaccacgc cggtgagcgc cgggatcgcc
     4921 gtgccgcgcc gttcagcgtt cgtttggccg cgatgcagat actcgcccag cagacgggtg
     4981 gccaggcgcg cgatcagcca ggtgaatgat accgctatcg ccacatgcaa cgcgcgccac
     5041 acccacaggt cggccctggc cggccagttc aagtgctgat agccgaacag gaagcccagc
     5101 accgtgatga tgagcaccac cgggctgcgc aattcgcgca acagcatgtc gtcggtgccg
     5161 gtgctggagc gtgcggccca gcggcccaac aggcccttcg ccacacgcga taccacctgc
     5221 cccagcagcc atccgcccag gacgaacagc agggccatga accagtcgtc ggtgctgttc
     5281 atcagcagca gttccttcca ggtgttgttg cgcatgttcc gttctttgcc ggtatcgcgg
     5341 cttgccgcac caaaggaaat gaacaccatg cctgttggcc ctgctccccg tccgcttgtc
     5401 gttgttaccg gcgccgggcg cggcgtgggc ttcgccaccg cgctggcact ggtccgcacg
     5461 caccgggccc aggtgatcgc cgtgtcccgc gatgtgtccg cgtggaatgc ggtgaaagag
     5521 gagggcatca caccgctgac tgctgatgtg gcaaccgagg aaggccgggc gaccgttgtt
     5581 cgggcggtgg acggacgccc tctcgctggc ttggtcaata atgccggctt gctgctcaag
     5641 cgcaacttgg gcacatggac cgctgcggac ctggagcggt tgcacgcggt gaacgtggcc
     5701 gcacccctgc tgttgacgca agccttgttg cccgcgctgc gtgccgcatc atccgcccat
     5761 gtggtcaaca tcggcagcat gggcggtttt caggggagta tcaagttccc gggtctcgtg
     5821 ggctacagcg ccagcaaggc cgccctggcc tgcatgagcg aatgcctggc cgaggagtgg
     5881 aaagacacca ccgtacgttg caattgcttg tgcctgggtg cggtggatac cgacatgttg
     5941 cgcgaggctt tccctggtta tgtggccccg gtctcggtcg gcaccatggg cgggtacatc
     6001 gccgccttgt agagcgagag gtgacccgag cgctggtagg tgatgccgcg ggtcatgtgc
     6061 ggggtgcgga cgcgctggta gccgtcccgg aactcccact cgcgcgcgag cttctcgagc
     6121 tcgtcgcaga tcaccgcgcc gttcgggagc cagagcacga ggcccttgcc gaccgcgagc
     6181 agctgctgca cgacgttcgg caggccgagc gtctcgagct gcggcggcgc gccggcgacg
     6241 gcgcggaacg cggcgccgag gccgccggag tggcggtaca cgttcacgcg gaagcggccg
     6301 tgcggcgagt cgtacgcgaa gtcgacggcc gtctcgctct cgagcgtctc gcgctgcacc
     6361 ggcgtcatga actcggtgag gaactcgagc acgtcgtggc tcgacagctc gcggaagcgc
     6421 acgggctgca gctcgcccca gat
//
LOCUS       DAOQOC010000019         6402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4701_length_6402_cov_4.163227, whole genome shotgun sequence.
ACCESSION   DAOQOC010000019 DAOQOC010000000
VERSION     DAOQOC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4701_length_6402_cov_4.163227"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PL106_00605"
     CDS             <1..443
                     /locus_tag="PL106_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA31592.1"
                     /translation="HRLEAADLLQEGFIRVFGKPEGFRMEGSLEGWIRRIMVHTCINH
                     YRTKAFQNERFGLEHVPEGVEEAVVLDHLGAEELMALVRELPDGYRMVFNLYAIEGYD
                     HAEIAEMVGCVESTSRSQLAKARRMLQQRIFEQAHPVDERKASH"
     gene            421..2007
                     /locus_tag="PL106_00610"
     CDS             421..2007
                     /locus_tag="PL106_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31593.1"
                     /translation="MNGKHPIDDHFARVLREAEATPPDAVWKNVAGAMDRPPARAYRY
                     RRITIALLLLLLLGGAAYWSVTSRGTRPDGPGTRQVRSTPPTAAPQAERSVEVGEHAQ
                     AEPSSTGRSQELGTVATPGSASDNKVSPVARERNTDADPSVPNRTHERPVGDGGGTER
                     DGRQASVGAVLNSSIEGGSRTDQPEHTGTAGLENEEGPAPEAASPVTEQPTPLFHAGG
                     LSGSSSTAESALPRSTPDVHLPRLASNPTPVVIRTAPTTGALMHGDSTPAYLLRKGRW
                     WFALQGGIAKLDGHWSGSDPAARELNRDETWLGGRSLGLVVGREWLNGWSAGLGIGAA
                     WQRARFLRLERTPGPTETVIDTTWTNSFQGPNAYWTWNIVETDVTEPGTQREFRATNE
                     YLWLRVAPEAARQLFEYRRLSLHARAGAAALFGITRKGSTLYRPSVHTLDAPPEGHVN
                     VVDLNDAAVRERFACSFALWGALELQYRVREHWSVGALPMVEWRPAMHSSKGPALSMT
                     SFGGALRLRYDLAHQEKRTR"
     gene            2046..3164
                     /locus_tag="PL106_00615"
     CDS             2046..3164
                     /locus_tag="PL106_00615"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31594.1"
                     /translation="MLCAALVIATASVAQCPGIRPVFSWASDGAGVVFTDLTEHPGVN
                     ITERHWVIGGVDTAQLITTTHVFDSAGVDSVHLIMMAEGCYYSVSALVGHAGDTETCT
                     SSISSTFSYVETGNNQLALTDQSTSDAPVLLYLWAFGDGNTSFDQHTEHFWPLPGAYD
                     VSHSIAGLNDQLQTTCVAGRAEHVFVDGNASTCDTSLFLGLDAEANGPLVTLNAQQVL
                     FNPDLTITGWQWDYGDGSPIDITSTSSAEHYYPYGGVVQICLRVTASDSLGNTCFARA
                     CTTVSMLEAVGLNGTAMPAELHARPVPCDDDLWVEGEAVRAGRSWHIVDALGREAATG
                     TFSLDDPERIDVHFLPVGLYTLTIRTEQGLRSIRVLKQ"
     gene            3246..>6402
                     /locus_tag="PL106_00620"
     CDS             3246..>6402
                     /locus_tag="PL106_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31595.1"
                     /translation="MKHLCASLVLLCSAYAVSAQPDCSQILNVGFSFGTQGNTVVLNN
                     GTQTEFPNATTYKWDFGDGELSTDPNATHVYDLPGTYLVCLYGMVENCMDSVCQEVVI
                     TGGENPCDDLSADFTVTTQGVVVVFDASGPATGYHWVFGDGMQSFDSDPVHTYPEPGT
                     YHACLVTWAWNPQTQDTCYAEHCEWVTIGDSSPCDDLVADFTVTTQGPVAAFNGYGGT
                     AVGYLWYFGDNTQGDGSSPTHTYPGPGSYNACLLTWAWNSNTQDTCWAEYCEEITIDG
                     VGPCDGLHAGFSFNTFPFGAQFSNSTTGTGFQTAWTWSFGDGTTSNDAQPVHTYSEPG
                     TYITCLTVTSIFEQQGGGVITCLDSICQEIHVQGQGSPCDDLIAAFEVTTQGNMADFT
                     ASGGSAVGYHWNFGDGTEGDGPAPMHTYPEAGSYHACLVTWAWNPQTQDTCYAEHCEW
                     VTIGGNSPCDGLQAGFQAQVGDVTVWFSNTTIGTGFQTSWHWDFGDGTTSDDAQPSHV
                     FPGLGTYEVCLTVVTLFEQQGGGVITCQDSYCSLVSNGGGSPCDSLIADFTVTTQGNT
                     ATFSAVGGTAVAYHWNFGDGTEEDGPSPAHTYPEAGSYHACLVTWAWDPQTQDTCYAE
                     HCEWVTIGDSSPCDDLVADFTVTTQGPVAAFNGYGGTAVGYLWYFGDNTQGDGSSPTH
                     TYPGPGSYNACLLTWAWNSNTQDTCWAEYCEEITIDGVGPCDGLHADFEADADGSDLY
                     FYSTSTGTGFQTSVVWSFGDGTTSTEAQPVHTYPEPGFYVACLTITSSFEQQGGGVIT
                     CTDSICQEVHTQGAGSPCELLNPGFEADVIDEPSVSFTSLTQGTGFQTSYLWSFGDGT
                     TGDGAQTVHTYAQPGTYLVCLTVTSLYEQQGGGVITCTDSACIELEVQVQDSLCNDLN
                     ADFESDADGQLVYFSNTSTGTGFQTGLTWFFGDGTTSNDDEPVHTYPEPGTYIACLTV
                     ISSFEQQGGGVISCMDTICHVIDIPLGLNGPAGSGTLDLWPQPFEDHFTLKGTDLSGM
                     TRFTLFEMTGQLVDDRSVGVQG"
BASE COUNT         1267 a         2057 c         1905 g         1173 t
ORIGIN      
        1 gccatcggtt ggaagccgcc gacctcttgc aggaaggctt catacgcgtg ttcggcaagc
       61 cggagggctt tcgcatggaa ggctcgctgg agggttggat ccgccgcatc atggtgcata
      121 cctgcatcaa ccactaccgc accaaggcgt tccagaacga acgcttcggt ctggagcacg
      181 tgcccgaggg cgtggaagag gccgtggtgc tcgatcacct gggcgccgaa gaattgatgg
      241 cacttgtgcg ggaactgccc gacggctacc gcatggtgtt caacctgtac gcgatagaag
      301 ggtatgacca cgcggagatc gccgagatgg tgggttgcgt ggaaagtacc agccgcagcc
      361 aactggccaa agcacgacgc atgctgcaac aacggatttt cgaacaagcg caccccgtag
      421 atgaacggaa agcatcccat tgacgatcac ttcgcacgtg tgctgcgcga agctgaggcc
      481 acaccgcccg atgcggtgtg gaagaacgtg gccggggcga tggaccgccc accggcgcgg
      541 gcataccgct accgcaggat caccatcgcc ctgctcctcc tgctgttgct gggcggagcc
      601 gcctattggt cggtcacatc gcggggaacc cgcccggacg gccctggcac ccggcaggta
      661 cggagcacac caccgaccgc cgcaccacag gccgaacgat cggtggaggt aggcgaacat
      721 gcgcaagcgg aaccgtcctc gacgggaagg tcgcaggagc tgggcacggt tgcaacgccg
      781 ggtagcgctt cggacaacaa ggtatctcct gttgcccggg aacggaacac cgacgcggat
      841 ccaagcgtcc cgaacaggac ccatgaacgg cccgtcggtg atggcggagg aaccgaacgc
      901 gatggccgcc aagcatcggt aggagccgtg ttgaatagtt ccatcgaggg gggatcacgc
      961 accgaccagc cggaacacac agggaccgcg ggccttgaga acgaggaagg accggcacct
     1021 gaagccgcat cgcctgtaac agagcaaccc acaccgttgt tccacgcggg gggcctgtcc
     1081 gggtcctcct ccacagcgga gtccgctctg ccgcgcagta cgcccgatgt gcatctgcct
     1141 cgtctggcaa gcaacccaac gccggtcgtg atccgaacag ccccaacgac cggagcactg
     1201 atgcatggcg atagcacacc agcgtacctc ttgcgcaaag gccgttggtg gttcgccctg
     1261 caaggcggta tcgccaaact cgacgggcac tggagcggct ccgacccggc ggcccgcgaa
     1321 ttgaaccgtg acgaaacctg gctcggcggc cgatcgctcg ggttggtggt gggccgtgaa
     1381 tggctcaacg ggtggtccgc aggcctgggt atcggagccg catggcaacg cgcgcgcttc
     1441 ttacggctgg agcgcactcc ggggccgaca gaaaccgtga tcgataccac ctggacgaac
     1501 tccttccaag gaccgaatgc gtactggacc tggaacatcg tggagaccga tgtgaccgag
     1561 cccggcaccc aacgcgagtt ccgcgcaacg aacgaatacc tgtggctccg cgtggccccc
     1621 gaagccgctc gtcagttgtt cgaataccgc cgactttcgt tgcatgcccg tgccggtgcc
     1681 gccgcactgt tcggtatcac ccgaaaagga agcaccctgt accggccctc cgttcacacc
     1741 ctggatgcgc cacccgaggg ccatgtgaac gtggtggacc tgaacgacgc tgccgttcga
     1801 gagcgcttcg catgctcctt tgcgctgtgg ggcgcgctgg aactgcagta tcgtgtgagg
     1861 gagcactggt cggtgggggc cctgcccatg gtcgaatggc ggcccgccat gcacagcagc
     1921 aaaggtcctg ccctgtccat gacatctttc ggtggcgcct tacgcctgcg ctacgacctg
     1981 gcccatcaag aaaaacgcac gcgatgaagg gctgcctgca aagttggcat tcatcacgcg
     2041 cagccttgct gtgcgcagca ctggtcatcg ccacggcttc cgtggcccag tgtccgggga
     2101 tccgcccggt gttctcctgg gcgagcgacg gggccggggt cgtattcact gacctcacgg
     2161 agcatcccgg ggtgaacatc acagaacgac actgggtgat cggcggtgtg gacaccgctc
     2221 aactcatcac caccacgcat gtatttgatt ccgccggggt cgatagcgtc cacctcatca
     2281 tgatggccga aggatgctac tacagtgtga gtgcgctggt gggacacgcg ggcgataccg
     2341 agacctgcac ctcttcgatc tcgtcgacct tcagctatgt ggaaacaggc aacaaccagc
     2401 tcgccctcac cgatcagagc acgagcgacg cgccggtgct gctgtacctg tgggccttcg
     2461 gcgacggcaa caccagcttc gatcagcaca ccgaacactt ctggcccttg ccaggcgctt
     2521 acgatgtgtc acattccatt gccgggctga acgaccagtt gcagaccacc tgcgtggcag
     2581 ggcgcgccga acacgtcttc gtggatggca acgccagcac ctgcgatacg tccctcttcc
     2641 tcggtctgga cgccgaggcg aacggccctc ttgtcaccct gaacgcccaa caggtcctgt
     2701 tcaacccgga cctgaccatc accggatggc aatgggacta tggtgatggc tcgccgatcg
     2761 acatcaccag cacgtcaagc gctgaacatt actaccccta tggtggcgtg gtgcagatct
     2821 gccttcgggt cacagcttcg gactccctgg gcaacacctg cttcgcgcgt gcatgcacca
     2881 ccgtgtccat gctggaggct gttggcctca acgggaccgc aatgccggcc gagttgcacg
     2941 cacgtcccgt tccttgtgat gatgatctgt gggtcgaggg cgaggcggta cgcgccggca
     3001 ggtcctggca tatcgtggac gcactcggtc gcgaggccgc aacgggcacc ttctccctgg
     3061 atgatcccga acggatcgat gtgcatttct tacctgttgg gctgtacacg ctgaccatcc
     3121 gtacagaaca aggcttgcgt tccattcgcg tgctcaagca ataggcaacg gaaccacgct
     3181 gtccgggcgt cctacacaca cgtcgcatga cgcccattcc ttcatgcggc cctcaactcc
     3241 acctcatgaa acacctctgc gccagccttg ttctgctctg tagtgcctac gctgtatcgg
     3301 cccagcccga ttgttcccag atcctgaacg tgggattttc gttcggcacg cagggcaata
     3361 cggtggtcct gaacaacggt acacaaacgg agttccccaa cgctaccacc tacaaatggg
     3421 acttcggcga tggtgagctg tccacagatc cgaacgcgac ccatgtgtac gacctcccgg
     3481 gcacttacct ggtctgcctc tacggtatgg tggagaactg catggacagc gtgtgccaag
     3541 aagtggtcat taccggaggt gagaacccgt gcgacgacct gagtgctgac ttcaccgtga
     3601 ccacacaagg ggtggtcgtc gttttcgacg catcaggtcc tgccacaggc taccactggg
     3661 tctttggtga tggcatgcag agcttcgata gtgacccggt ccatacctat cccgagcccg
     3721 gcacctatca cgcttgcctg gtcacctggg cctggaaccc gcagacacag gacacgtgct
     3781 acgccgagca ttgcgagtgg gtcaccatcg gcgacagttc cccgtgcgat gacctggtgg
     3841 ctgatttcac cgtgaccaca caaggccctg tggccgcttt caacggatac ggcgggacgg
     3901 ctgtgggtta cctgtggtat ttcggcgaca acacacaggg cgatgggtca tcgcctacgc
     3961 acacctatcc cggcccggga tcatacaacg cttgtctgct cacctgggca tggaacagca
     4021 acacccagga cacctgctgg gccgaatatt gcgaggagat cacgatcgat ggtgtcggac
     4081 cgtgtgacgg tctgcacgct ggattctctt tcaacacctt tccgttcgga gcacaattct
     4141 ccaactccac caccggtacc ggcttccaga ccgcgtggac ctggtccttc ggcgatggca
     4201 ctaccagcaa tgatgcccag ccggtacaca cctactcgga acccggcacc tatatcacct
     4261 gcctcaccgt caccagcatc ttcgagcagc agggcggcgg agtgatcacc tgcctggact
     4321 ccatctgcca ggagatccac gtccaaggac aaggatcccc ctgcgatgat ctgatcgccg
     4381 cgttcgaggt gaccacccaa gggaacatgg ccgacttcac ggcgtccggt ggttcggccg
     4441 tcggctacca ctggaacttc ggtgacggaa cggaagggga tggtcccgct cccatgcaca
     4501 cctaccccga agcgggtagc taccacgcct gcctggtcac ctgggcctgg aacccgcaga
     4561 cgcaggacac gtgctacgcc gagcattgcg agtgggtcac catcggcggc aattcgccgt
     4621 gcgatggcct tcaggccggc ttccaagcac aggtcggtga cgtaacggta tggttctcca
     4681 acaccacgat cggcaccggc ttccaaacat cctggcattg ggatttcggt gacgggacca
     4741 cgagcgacga tgcgcagccc tcccacgtct tccccggcct gggtacatac gaggtctgcc
     4801 tgacggtcgt cacccttttc gagcaacaag gtggcggggt gatcacttgc caggacagct
     4861 attgctcact tgtttccaat ggcggtggtt cgccgtgcga cagcttgatc gccgatttca
     4921 ccgtgaccac ccaaggcaac accgctacct tctccgctgt aggaggtacg gccgtcgcct
     4981 accactggaa cttcggagat ggaacggaag aggatggtcc ctctcccgcg cacacctacc
     5041 ccgaagcggg tagctatcac gcctgcctgg tcacctgggc ctgggatccg caaacgcagg
     5101 acacatgcta cgccgagcat tgcgagtggg tcaccatcgg cgacagttcc ccgtgcgatg
     5161 acctggtggc tgatttcacc gtgaccacac aaggccctgt ggccgctttc aacggatacg
     5221 gcgggacggc tgtgggttac ctgtggtatt tcggcgacaa cacacagggc gatgggtcat
     5281 cgcctacgca cacctatccc ggcccgggat catacaacgc ttgtctgctc acctgggcat
     5341 ggaacagcaa cacccaggac acctgctggg ccgaatattg cgaggagatc acgatcgatg
     5401 gtgtcggacc gtgtgacggt ctgcacgccg acttcgaggc cgatgcggac ggctcggatc
     5461 tgtacttcta cagcacctcc accggcacgg gtttccagac cagcgtggtc tggtccttcg
     5521 gtgatggcac caccagcacc gaagcccagc cggtacacac ctacccggaa cccggcttct
     5581 atgtcgcctg cctcaccatc accagcagtt tcgagcagca gggtggcggg gtgatcacct
     5641 gcacggattc catctgccag gaggttcaca cgcagggggc ggggtcccct tgtgaattgc
     5701 tgaatcccgg gttcgaggcc gatgtgatcg acgagccgtc agtgtccttc accagcctca
     5761 cgcagggtac gggtttccag accagctatc tctggtcctt cggcgatggc accacaggcg
     5821 acggggccca gacggtacac acctatgcgc agcctggcac gtatctcgta tgccttaccg
     5881 tcaccagcct gtacgagcag cagggcggag gggtgatcac ctgcacggac tcggcatgca
     5941 tcgaactcga ggtccaagtg caagactccc tctgcaatga tctgaacgcc gacttcgaga
     6001 gcgacgccga tggccaattg gtttacttca gcaacacctc caccggaacg ggtttccaga
     6061 ccggcctgac ctggttcttc ggcgatggca ccaccagcaa cgatgacgag cctgtgcata
     6121 cctatcccga gcctggcacc tatatcgcct gcctcaccgt catcagcagc ttcgagcagc
     6181 agggtggcgg ggtgatctcc tgcatggaca ccatctgcca tgtgatcgac attccgctcg
     6241 ggctgaacgg tccggccgga agcggaacac tggacctgtg gccacagccc ttcgaagatc
     6301 acttcacctt gaagggtacc gacctgagtg gtatgacccg cttcaccttg ttcgagatga
     6361 ccgggcaact ggtcgatgat cgaagtgttg gtgtacaagg cc
//
LOCUS       DAOQOC010000020         6287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4824_length_6287_cov_3.712452, whole genome shotgun sequence.
ACCESSION   DAOQOC010000020 DAOQOC010000000
VERSION     DAOQOC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4824_length_6287_cov_3.712452"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PL106_00625"
     CDS             complement(<1..920)
                     /locus_tag="PL106_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNA31596.1"
                     /translation="MQQKLEQWKDLKFGLLMHWGPYSQWGVVESWSICPEDEGWCQRK
                     PEHGATYNEYVKNYEALQTTFDPVMFDPAKWARAAKDAGMKYVVFTTKHHDGFCMFDT
                     KLTDYRITDPKCPFHDDPRANIAMGVFDAFRTEGLWTGAYFSKPDWHSPYYWWPYFPP
                     KDRNVNYLPTKYPERWRQFKDFTYGQIEELMTGYGPMDILWLDGGQVRPLNTVDTTVS
                     WQKAIQIEQDIDMARIAAMARKHQPGILVVDRTVGGEYENYVTPEGHVPDADLGVPWE
                     TCMPMGTSWSWVPNERYKSTDDIIRTLVKVV"
     gene            1162..1803
                     /locus_tag="PL106_00630"
     CDS             1162..1803
                     /locus_tag="PL106_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA31597.1"
                     /translation="MSTVRIIVADAQDLVADGLRYRLADASGLMVAAHVRSGTDLFEL
                     LAQRKADLVLLDVSLPGMDGIDTARALRKKHPDVKVLAHSMRTEVEYVNSMLIEGAAG
                     YLLKNTPKTELLEAIATVMKGGRHISEEARRSIEKGYKFTEKRPDGAYIGLTEREREI
                     IRMIALERTNDEIALALHLSTDTVKTHRKHLMTKLNVRSAAGLVKYAVDRMWI"
     gene            1927..2460
                     /locus_tag="PL106_00635"
     CDS             1927..2460
                     /locus_tag="PL106_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31598.1"
                     /translation="MKTRHNGSLLSNVLAAILSFMGIACFALAASAEVDMVTIQGQLR
                     ADDLPLTDAVVVVEVKTDEVCLRSELSVDGRFKFAVPVGAKARLFFIKPGYLTKEVEV
                     DTKNALTTDEARKFNKKVKFDVVLEEELLHVGETYFGPVGQIHFVNGTGLMRVTHDGR
                     MKRLDPTSLQANAVRKD"
     gene            complement(2543..3289)
                     /locus_tag="PL106_00640"
     CDS             complement(2543..3289)
                     /locus_tag="PL106_00640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA31599.1"
                     /translation="MNEQDWDNVATTFEKEIFNVPANDTKGHIIGWVDRLAVPGAVAT
                     DLGCGVGRTLPMLSDRYDKVLAVDVSAQCLEVAARACEGRTNIRYVHADLSRDRNGYP
                     ATDVVLCINTLLNASLEIRGALVDRTCRSVRKGGHLLLVVPSLNSALLTAYRHLQWNL
                     RQGMSAKRAQKEAALNSEGLEHGIVRIDGVPTKHYLQEELETMLAANGYEVLAIEKIE
                     YPWNTEFDAPPKWMKAPWPWDWFVAARRVR"
     gene            complement(3451..4872)
                     /gene="pyk"
                     /locus_tag="PL106_00645"
     CDS             complement(3451..4872)
                     /gene="pyk"
                     /locus_tag="PL106_00645"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNA31600.1"
                     /translation="MNEPKTKIVATIGPASASRAVLRELMLSGMDVARLNFSHGSYEF
                     YRQVITDIRDLNAELGINTAILADLQGPKLRVGEMENGAIDVPDGSELVITNEPMVGR
                     PGRVSTVYRQFPQDVRPGETVLLDDGKLRMQVLSTDGHKEVRLKVLSGGSLKDKKGLN
                     LPNTKVSLPSLSEKDRSDLDFALSQDVDWVGLSFVRSARDIIELKRIITSQDKHARVV
                     AKIEKPEALEEIDGIIRESDALMVARGDLGVEVPMEKVPLIQKDIIRRCLREGRPVIV
                     ATQMMESMIENSSPTRAEVNDVANAVLDHADAVMLSAETSVGRHPVEVVRTMNRILRE
                     METSDGMFNVPAADLRMNERSISDAISIAAVRMTKALSVQAIVTMTHSGYSAFKISSM
                     RPKANIFAFTSNPRILCMLNLVWGVRAYYYDKTVSTDHTIADIKHLLRKRKQVSDGDL
                     VINIASMPIAEKGMSNMLKLSQA"
     gene            complement(4869..5279)
                     /locus_tag="PL106_00650"
     CDS             complement(4869..5279)
                     /locus_tag="PL106_00650"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNA31601.1"
                     /translation="MAKHKLDMDPDPEVMLIGISCHENDYRLCWALNRELGINLARRS
                     SDVTAPGPEKLASYSAFDHDDEHEAHWTLVHNHSNDGVLLKEHKQADYFLIVDESAPI
                     TPQELIERVRRTEFVLTAYPLDARTERGAHILLR"
     gene            complement(5325..>6287)
                     /gene="rnc"
                     /locus_tag="PL106_00655"
     CDS             complement(5325..>6287)
                     /gene="rnc"
                     /locus_tag="PL106_00655"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNA31602.1"
                     /translation="TPDPLFSALFSRPKNPEERKIRAWCRQKLGITPGDLALYRQALR
                     HSSAVPEDRPDLPNNERLEFLGDAILDAIVGQFLFNKYPDQGEGFLTRMRSKLVSRHQ
                     LNILARFVEIEKVVESNVGRGHETSVPGNALEALIGAIFLDKGFDRTNKTVLALIQGN
                     LDLKTVEQEDRDSKSRLLEWGQKRKHKVEFRVVEEPGRGGRGKLYIAEACVDGQVRGT
                     GRGQSKKKAEQDAANAAYRSLRPHGDRDDQGRSEVDAHRRSRPRGQQRGARDRDRRAQ
                     PPDGPASSGSSGIQGPKAALPPHQGQLRAGSTSGGTQEDAPAKA"
BASE COUNT         1099 a         2021 c         1885 g         1282 t
ORIGIN      
        1 acgactttca ccagcgtgcg gatgatgtcg tcggtgctct tgtaccgctc gttcggcacc
       61 caactccagc ttgtgcccat gggcatgcag gtctcccacg gcacaccgag gtcggcatcg
      121 ggcacatgcc cttcgggcgt cacgtagttc tcgtactcgc cgcccacggt gcggtccacc
      181 accaggatcc cgggctggtg cttgcgcgcc atcgctgcaa tgcgagccat gtcgatgtcc
      241 tgctcgatct ggatcgcttt ctgccaactc acggtggtgt ccacggtgtt caacggccgc
      301 acctgtccgc catcgagcca gaggatgtcc atgggtccgt agcccgtcat cagttcctcg
      361 atctggccgt aggtgaagtc cttgaactgc cgccaacgct cgggatactt ggtgggcagg
      421 tagttcacat tgcggtcctt cgggggaaag tagggccacc agtagtaggg actgtgccag
      481 tcgggtttgc tgaagtaggc gccggtccac aggccctcgg tgcggaacgc atcgaagacg
      541 cccatggcga tgttggcgcg cgggtcatcg tggaaggggc acttcggatc ggtgatgcgg
      601 tagtccgtga gcttcgtatc gaacatgcag aagccgtcgt ggtgcttggt ggtgaagacc
      661 acgtacttca tgccggcatc cttcgcggcg cgcgcccact tcgcggggtc gaacatcacc
      721 ggatcgaagg tggtctgtag cgcctcgtag ttcttgacgt actcgttgta cgtggcgcca
      781 tgctctggtt tgcgctggca ccagccttca tcctccgggc agatgctcca gctctccacc
      841 acgccccatt ggctgtacgg cccccagtgc atcagcaggc cgaacttcag atccttccat
      901 tgctccaact tctgctgcac caagggatcg gagggcggga cgtagggggc ttgggcccaa
      961 agctgctgaa gtccaaggac gccgatcaaa agcaaccgtg ctctcacttc gccaaactac
     1021 acagtggatc ggctccgcaa tttccaagat caccgcttgc gggagccacg aaggcggtct
     1081 acttcatctt caggctggtc gcgatggcac ttgcgcgcag gtcttccgtg ctgctcacgg
     1141 cgtatgaccg atattcgcgg aatgtccacc gtgcgcatca tcgtggccga tgcccaggac
     1201 ctggtggccg acggcctgcg ataccggctg gcggatgctt ccggcctgat ggtcgcggct
     1261 catgtgcgtt cgggcacgga tctcttcgag ctgcttgccc aacgcaaggc cgatctggtg
     1321 ctgctggatg tgtccctgcc cggtatggat ggcatcgata cggcgcgagc gttgcgcaaa
     1381 aagcacccgg acgtcaaggt tctcgcgcac tcgatgcgca ccgaggtcga gtatgtgaac
     1441 agcatgctca tcgagggcgc agcgggctac ctgctgaaga acacgccgaa aaccgaactc
     1501 ctggaggcca tcgccaccgt tatgaaaggc ggacggcaca tcagcgagga agcgcgacgt
     1561 agcatcgaaa aggggtataa attcaccgag aaacgaccgg atggcgcata catcggcctt
     1621 accgaacgcg aacgcgagat catccgcatg atcgccttgg agcgtacgaa tgacgagatc
     1681 gccctggcat tgcacctgag cacggacacg gtgaagacgc accgcaagca cctgatgacc
     1741 aaactcaacg tgcgaagtgc ggccggcctg gtgaagtacg cggtggaccg catgtggatc
     1801 tgaccctgcg gatccggcgg tttcactcct tgtgggtatt gtgacggcct ggttgacacg
     1861 tgacatttgt cccgtcgcac cagcgtactc ccccccaacg cttcgccagc ccaggcggcc
     1921 cagaaaatga aaacccggca caacggcagc ctcttgagca atgtcctcgc cgccatcctg
     1981 tccttcatgg ggatagcctg tttcgccctg gcagccagcg ccgaggtcga catggtaacg
     2041 atccagggcc agttgcgtgc cgatgacctg ccactgaccg acgccgtggt ggtggtggag
     2101 gtgaaaaccg atgaagtgtg cctacgcagc gaactcagcg tggacggtcg tttcaagttc
     2161 gccgtgcccg tgggggccaa agcccggctc ttcttcatca agcccgggta cctgaccaag
     2221 gaagtggaag tggacaccaa gaacgccctg accaccgacg aggcccgcaa gttcaacaag
     2281 aaggtcaagt tcgatgtggt gctcgaagag gaactcctgc atgttggtga gacctatttc
     2341 ggccccgtgg ggcagatcca cttcgtgaac ggcaccggtc tgatgcgcgt gacccacgac
     2401 gggcgcatga agcggttgga tcccacctct cttcaggcga acgcggtgcg caaggattga
     2461 accacgacgg gcgcccggaa gacgagcccg gcctcccgtt gacacggccc tcgcagggat
     2521 gcgggggccg tgttccttcc ggtcaccgca cgcgacgggc ggcaacgaac cagtcccacg
     2581 gccacggcgc cttcatccac ttcggcggtg cgtcgaactc ggtgttccag gggtactcga
     2641 tcttctcgat ggccaacacc tcgtagccgt tcgcggccag catggtttcc aactcttcct
     2701 gcagatagtg tttggtcggt acgccgtcga tacgcacgat gccgtgttcc aatccctcgc
     2761 tgttgagtgc ggcctctttc tgtgcccgct tggcactcat cccctgccgc aggttccatt
     2821 ggaggtgcct gtacgccgtg agcagggccg agttcagcga aggaaccacc agcagcagat
     2881 gcccaccctt cctcacgctc cggcatgtgc ggtcgaccaa tgcaccgcgg atctccaagg
     2941 aggcattgag cagggtattg atgcacagca ctacatcggt cgccgggtag ccgttcctgt
     3001 ccctgctcag gtccgcatgg acgtacctga tgttggtgcg gccttcgcag gcgcgcgccg
     3061 ccacctcaag gcactgggcg ctcacgtcca ccgcgagcac cttatcatac cggtccgaca
     3121 gcatcggcaa ggtgcggccc acgccgcatc caagatccgt tgcaacagcc cccggtacgg
     3181 ccagccggtc cacccagccg atgatgtgac ccttggtgtc gttggccggt acgttgaata
     3241 tctccttctc gaacgttgtg gccacgttgt cccagtcctg ttcgttcatg cgtgtggtgt
     3301 tatgccacgg cacctgcccg gtccaagaat gggaatgtcc ggtgcgcgcg gcggatccgc
     3361 gatcgtcggc aatgtatccg ccgcgggccc ccatcggacg cctgcacgcc catgcgttgt
     3421 acacgggcga tcgttgatcc ggaaaatata ctatgcctgg ctcagcttga gcatgttgct
     3481 catccccttc tcggcgatgg gcatgctggc gatattgatc accagatcgc cgtccgacac
     3541 ctgcttccgt ttgcgcaaca ggtgcttgat gtcggcgatc gtgtgatccg tgctcaccgt
     3601 cttgtcgtag tagtacgcgc gtactcccca caccagattg agcatgcaca gtatgcgcgg
     3661 gttgctggtg aaggcgaaaa tgttcgcctt gggccgcatg ctgctgatct tgaacgccga
     3721 atagccgctg tgcgtcatgg tcacgatggc ctgaacggaa agcgccttgg tcatgcgcac
     3781 cgccgcgatg ctgatggcat cactgatgga gcgttcgttc atgcggaggt cggcagccgg
     3841 cacattgaac atgccatcgc tcgtctccat ctcgcggagg atccggttca tggtacggac
     3901 cacctccacc ggatgccggc ccacactggt ctcggcgctc agcatgaccg catcggcatg
     3961 gtccagcacc gcattggcca catcgttcac ctcggcacgg gtggggctgc tgttctcgat
     4021 catgctctcc atcatctggg tggccacgat caccggccgc ccttcgcgca ggcagcgccg
     4081 gatgatatcc ttttggatca gcgggacctt ttccatgggc acctccacac caaggtcgcc
     4141 ccgggccacc atcaaggcat cgctctcgcg gatgatgcca tcgatctcct ccagtgcctc
     4201 gggcttctcg atcttggcca ccacccgcgc atgtttgtcc tgcgaggtga tgatccgctt
     4261 gagctcgatg atgtcgcggg cactcctcac gaaactgagg ccgacccagt caacatcctg
     4321 ggagagtgcg aagtccagat cgctccggtc cttctcgctc aggctgggca aggagacctt
     4381 ggtgttgggc aggttcaggc ccttcttgtc cttgagcgac ccgccgctga gcaccttgag
     4441 ccgcacctcc ttgtgcccgt cggtggagag cacctgcatg cgcagcttgc catcgtccag
     4501 cagcacggtc tccccggggc gcacgtcctg cgggaattgc cggtacacgg tgctcactcg
     4561 cccgggtcgt ccgaccatcg gttcgttggt gatgaccagc tcactgccat ccggcacatc
     4621 gatggccccg ttctccatct cgcccacgcg caacttgggg ccctgcaggt ccgcgaggat
     4681 ggccgtattg ataccgagtt ccgcgttcag gtcccggata tcggtgatca cctggcggta
     4741 gaattcgtaa ctgccatggc tgaaattgag gcgggccaca tccatgccgc tcagcatcag
     4801 ttcccgcagc acggcccggg aggccgaagc gggaccgatg gtggcgacga tcttcgtctt
     4861 cggttcgttc atctcaggag tatgtgcgcg ccacgttcgg tacgcgcgtc caggggatag
     4921 gcggtcaaca cgaattccgt ccggcgtacg cgctcgatca gttcctgtgg tgtgatcggc
     4981 gcgctctcat ccacgatcag gaaataatcg gcctgcttgt gctccttcag cagtacgccg
     5041 tcgttgctgt ggttatgcac cagggtccag tgggcctcgt gctcgtcatc gtggtcgaac
     5101 gctgaataac tggccagttt ctcggggccc ggcgcggtca catcgctgga acgtcgcgcg
     5161 agattgatgc ccagttcccg gttgagcgcc cagcatagcc ggtagtcgtt ctcatggcaa
     5221 ctgatcccga tgagcatgac ctccgggtcg ggatccatat ccagcttgtg cttggccacg
     5281 cgccaaatgt agcccgggcg aaaaatcgga gcgtcacccg cacctcatgc tttcgcaggc
     5341 gcatcttcct gcgttcctcc ggaggtggaa ccggcgcgca actgtccttg atgcgggggc
     5401 agtgccgcct tgggtccttg gatccccgat gatccgctgg acgctggacc atccggcggt
     5461 tgtgcccgcc tgtcgcggtc gcgagcgccg cgctgctgac cccgtggtcg cgagcgccgg
     5521 tgcgcatcaa cctcggaacg gccctgatcg tcacggtcac catgcggacg cagactgcgg
     5581 tacgcggcat tggccgcatc ctgctcggcc ttcttcttgc tctggccccg accggtacca
     5641 cgcacttggc cgtcaacaca ggcctcggcg atgtacaact tgccacgacc accccgcccg
     5701 ggctcctcca ccacgcggaa ttccaccttg tgcttcctct tctgtcccca ttccagcagt
     5761 cggctcttgc tgtcgcgatc ctcctgttcc accgtcttca ggtcaaggtt gccctggatc
     5821 agtgccagaa cggtcttgtt cgtgcggtca aaccccttgt ccaggaagat cgccccgatc
     5881 aaggcctcca gtgcgttgcc cggcacgctc gtctcatgcc cgcgtcccac gttgctctcc
     5941 accaccttct cgatctcaac gaaacgcgcc aggatattca actgatggcg gctcaccagc
     6001 ttgctgcgca tacgggtaag gaacccttca ccctgatcag gatatttatt gaaaaggaac
     6061 tgaccgacga tcgcgtccag gatcgcatcg ccgaggaact ccaaccgctc gttgttgggc
     6121 aggtcgggcc ggtcctcggg cacggcgctg ctgtggcgca aggcctgccg gtacagggcg
     6181 agatcgccgg gagtgatccc cagtttctgg cggcaccagg cccggatctt ccgttcctcg
     6241 gggttcttcg gccgactgaa gagggcgctg aacaaggggt caggcgt
//
LOCUS       DAOQOC010000021         6279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4832_length_6279_cov_3.441517, whole genome shotgun sequence.
ACCESSION   DAOQOC010000021 DAOQOC010000000
VERSION     DAOQOC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4832_length_6279_cov_3.441517"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..885
                     /locus_tag="PL106_00660"
     CDS             376..885
                     /locus_tag="PL106_00660"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNA31603.1"
                     /translation="MPFRTLAFSALLLSLVSACDSQKPAVDQPALTSIDPVQFAMAPD
                     SVFFQLRRTPCFGTCPAYTITILGNGRATYSGHSPVERKGEYNGQVDASIMRALYDRA
                     TSISFFEFEDKYDGPVTDLPSTIIRVSANGRDKRVVGRVKPPAAFKAFATYADTLLAG
                     VKWMPVDIK"
     gene            1048..3282
                     /locus_tag="PL106_00665"
     CDS             1048..3282
                     /locus_tag="PL106_00665"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNA31604.1"
                     /translation="MKKFFLLAVLLGTTILLRAQEGMWMLNTLKQVNESDMKRLGFQL
                     TADDVYNVNASSMKDAVARLGGGFCSGEIVSADGLMLTNHHCGYDAIQSLSSVEHDYL
                     TDGFWAKTRAEELPGGFYVSFLQRIDNVSERVLATLNDGMSEEERQAKIAEIGATLEQ
                     EAVGELKHISADFKTMYEGNEFYIFVYKDYPDVRLVGAPPSAIGKFGGDTDNWMWPRH
                     TGDFSMFRVYTGPDGEPAPPAAENIPFKPKYWFPISLEGVQEGDFSMVMGCPGSTDRF
                     LSSQGVQLALDVEQPSRVKIRRAKLDVYDKHMEADNAVRIQYASKYAQVSNYWKYFIG
                     QQRGLKRLHVFDKKKKLENDLSTWIDADPARKSKYGGYLSELEAGYAERAKYEKANTY
                     MQEAAFGCEALINGIRIHANGLYNELSAPKPDAAKVAAIATRIQRRSGTMWGDYNAPT
                     DQEVTAEMFRLMGKDLPADMRPAVINDVILKKFKGNYDAWAAALFKTSILCDSAKFNK
                     FMAAPSAKVLKKDLGFQAAQSAFDLFQQVGAASAGAQLRIDRGYRLMVAAMREREPGK
                     MWYPNANSTIRMSYGQVAGYKPGDAMHYDHITTANGILEKEDNTNDEFKVPAREHELL
                     VKRDYGRYADKSGELVPCFISRNDITGGNSGSPVINGKGQLIGIAFDGNWEAMSGDIA
                     YETELQRMISVDIRYVLWTIDKFAGAKHLVDEMTIAPPTPVAPPPAPVGDALAPKGSS
                     VPKK"
     gene            3377..4054
                     /locus_tag="PL106_00670"
     CDS             3377..4054
                     /locus_tag="PL106_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA31605.1"
                     /translation="MRILIADDDPLSADLLRAHCLRIDPAAEVVHALDGAQAQNLIRS
                     GRWDLLLLDLNMPKVDGRTLLETIGERLPVIIVTGDPAFALDAYRFHVHDYLVKPVTF
                     ERFASAWRALDRKAPKPSGGANNIVFVRSGSDIVQVDLDEVRYVKSESNYVRFVLDGR
                     DVLSLMNMKDLGGKLPGAFVRVHRSYFVNLRHVEKLDTNDIKVGRDLIPVSETYRQSL
                     IAQLALL"
     gene            complement(4280..>6279)
                     /locus_tag="PL106_00675"
     CDS             complement(4280..>6279)
                     /locus_tag="PL106_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA31606.1"
                     /translation="AGMVIINADMRALFAELLALADSGSVLVLARGDGEVILHPDTSQ
                     CFRFDLGGSRRMENVLPKEDGAEVLIGRREFELGPSKARFEMAITRSLSGLPGSLRHR
                     RNELVLLFAGIALGAIALTAMFALRFRERLNRLTGLMERYAVGSNEELPTARNDEFGR
                     MARGLRTMQERIDARVRELEQARVAAETSDRQRRELLANMSHEVRTPLNAIIGMGGEI
                     DTAHLGPADREHMAIIQRSAERLRGLVDDLLMHARIGEGKLALHPVPVDVRTLVNDVT
                     QAHLPSARTKGLRIHTILHDLPPALLLDPLRLHQIVDNLLGNAVRFTEVGQVTLEVGM
                     DGPGTLRIQVADTGPGIPANERERVFERFERASSSEQGHGAGLGLAITRRVTDLMGGR
                     ISMDSEVGRGTRFTVLLPTAATDPGSTVQVTAVETRGLRVLHVEDVATNRLLMRQWAA
                     RWNWALTEAESSGSARSACTAGTFDLLLLDLELGSGDSGIELARQLRREGRHRYTPIL
                     ALTAHASDDTDAEILLAGMNARITKPLDRDALAAAAAWWTDERDDGTIGIATLLDQYG
                     VSGTRAIDLIQQFRDEFAKHRVSIGQAITAGDADLLSRTRHQVRPHWQLLGLEKGLAA
                     LDALVIPVTPGTEAAVVHWFAACDRAMMREQRRSAGITIAG"
BASE COUNT         1201 a         1988 c         1952 g         1138 t
ORIGIN      
        1 ttactcagtc ggtggtcaag atccggtttg accggcgatc aaccatggtg ggtcgaaggg
       61 tgcaagagtg cgagagcgcg aggctcaaac ccttgcaccc tcgcactttt gaacccacgg
      121 atcgcccgct tctttatcac cgtatgagta ggtcttgcgg ggtaacaatg cccggttgtt
      181 gaaaagctca tgtttccggg aatgctctct atccatgagg acttggtgcg agcagggtga
      241 ccatggggag cggggaggtg ccggatgtgc ccttggtctc atgtgcccat gcgcaacgta
      301 ctcagttcgt gttcggcagg gttttggcat cccggtcggc cactaccttc gtcccggtcc
      361 acttgcaccc aagcgatgcc tttcagaacc ctcgcctttt ccgccctgct cctttcactg
      421 gtcagcgcct gcgatagcca gaaaccggcc gtggaccagc ctgcgctcac gagcatcgat
      481 ccggtacagt tcgccatggc cccggacagc gtgttcttcc agttgcgccg caccccctgt
      541 ttcggcacct gtccggccta cacgatcacg atcctcggga acggacgagc cacgtacagc
      601 ggccattcac ccgtggaacg caaaggtgag tacaacggcc aggtggatgc gtcgatcatg
      661 agagccctct acgatcgtgc cacaagtatc agcttcttcg aattcgagga caagtatgac
      721 ggcccggtga ccgacctgcc cagcaccatc atccgcgtga gtgccaatgg ccgcgacaag
      781 cgggtggtgg gccgtgtaaa gccgccggca gcgttcaagg ccttcgccac ttacgccgac
      841 acgctcctgg ccggggtgaa gtggatgccg gtggacatca agtagggtcc aaagattcaa
      901 ggtcccgtgg tcgcaggtgt tcccttcgac ctcgcccttg ccgttcatca caccggtcag
      961 ccccgccgaa gatcgggaag cctacattcg cgcccgcttt caattccctg aggcccaagc
     1021 agggccgcgt caccaacaac cgaatcgatg aagaagttct tcttgctggc cgtgctgctc
     1081 ggtacgacca tcctgctccg cgcccaagag ggcatgtgga tgctcaacac gctgaagcag
     1141 gtgaatgagt ccgatatgaa gcgcctcggg ttccaactca ccgccgatga cgtgtacaac
     1201 gtgaacgcca gcagcatgaa ggatgccgtg gcacgcctcg gtgggggctt ctgcagcggc
     1261 gagatcgtga gcgccgacgg actgatgctc accaatcacc actgcggtta cgacgcgatc
     1321 cagagcctca gcagcgtgga gcacgattac ctcaccgacg gcttctgggc caagacacgc
     1381 gccgaagaac tgcctggtgg gttctacgtg agcttcctgc agcgcatcga caacgtgtcc
     1441 gagcgcgtac tcgccacatt gaacgatggc atgagcgagg aagagcgcca ggcgaagatc
     1501 gccgagatcg gtgccacgct cgagcaggaa gccgttggcg aactgaagca catcagtgcc
     1561 gacttcaaga ccatgtacga gggcaacgag ttctacatct tcgtctacaa ggactacccc
     1621 gatgtgcgcc tggtgggcgc accgcccagc gcgatcggca agttcggtgg cgacaccgac
     1681 aactggatgt ggccccgcca caccggcgac ttcagcatgt tccgtgtgta caccggtccc
     1741 gatggcgagc ccgctccgcc cgctgccgag aacatcccgt tcaaacccaa gtattggttc
     1801 cccatcagcc tggaaggcgt tcaagaaggc gacttcagca tggtgatggg ctgccccggc
     1861 agcacggacc gtttcctcag cagccaaggc gtgcaactgg cacttgatgt ggaacagccc
     1921 agccgcgtga agatccgtcg cgccaaactc gatgtgtacg acaagcacat ggaggccgac
     1981 aacgccgtgc gcatccagta cgccagcaag tacgcgcagg tgagcaacta ctggaagtac
     2041 ttcatcggcc aacagcgcgg cctgaagcgt ctgcatgtgt tcgacaagaa gaagaagctc
     2101 gagaacgacc tgagcacttg gatcgatgcc gatcccgcgc gcaagtcgaa gtacggcggc
     2161 tacctgagtg agctcgaggc gggctatgcc gaacgggcca agtacgagaa ggccaacacc
     2221 tacatgcagg aggccgcctt cggttgcgag gccctgatca acggtatccg catccatgcc
     2281 aacggcctgt acaacgaact ctctgcgccc aagcccgatg ccgccaaggt ggccgctatc
     2341 gctacccgca tccaacgccg ttcggggacc atgtggggcg actacaacgc gcccacggac
     2401 caggaggtca ccgctgagat gttccgcctt atgggcaagg atctgcctgc cgacatgcgc
     2461 cccgcggtga tcaatgatgt gatcctgaag aagttcaagg ggaactacga tgcatgggcc
     2521 gccgccctgt tcaagacgag tatcctgtgc gacagtgcca agttcaacaa gttcatggcc
     2581 gcgccttcgg ccaaggtgct gaaaaaggat ctcgggttcc aggctgctca gagcgccttc
     2641 gatctcttcc aacaggtggg tgccgccagt gctggcgcac aactgaggat cgatcggggc
     2701 taccgcctga tggtggccgc catgcgcgaa cgcgaaccgg gaaagatgtg gtaccccaac
     2761 gccaacagca ccatccgcat gagctacggg caggtggcgg ggtacaagcc cggtgatgcc
     2821 atgcactacg atcatatcac cacggcgaac ggaatccttg aaaaggagga caataccaac
     2881 gatgagttca aagtgcccgc acgcgagcat gaattgctgg tgaagcggga ctacggccgg
     2941 tatgccgaca agagcggcga actggtgccc tgcttcatca gccgtaacga catcaccgga
     3001 ggcaacagcg gcagtcccgt gatcaacggc aagggccagc tcatcggtat cgccttcgac
     3061 ggcaactggg aggccatgag cggcgatatc gcctacgaga ccgaactgca gcgcatgatc
     3121 agcgtggaca tccgctacgt gctctggacg atcgacaagt tcgccggggc gaagcacctg
     3181 gtggacgaaa tgaccatcgc gccgcccaca ccggtagcac ccccgcccgc gccggtcggc
     3241 gacgcactcg cacccaaggg ctcgtcggtg ccgaagaagt agggcggatc cgctgggccc
     3301 acaagctccg gagcgttgca cagtgcgacg ctccggaact gtataaggca cctgcaccga
     3361 gctatcttgc accgccatgc gcatcctcat cgcggacgac gacccgttga gcgccgacct
     3421 gttgcgggcg cactgcctgc ggatcgatcc ggccgccgag gtggtacatg ccttggacgg
     3481 tgcccaggcc cagaacctga tccgttcggg ccgatgggac ctgttgctgc tcgacctgaa
     3541 catgcccaaa gtggatgggc gcaccttgtt ggagaccatc ggtgagcgcc tgccggtgat
     3601 catcgtgacc ggggatcccg ccttcgcgct ggatgcgtac cgcttccatg tgcatgatta
     3661 cctggtgaag cccgtcacct tcgaacggtt cgcctcggcg tggcgcgcgt tggaccgcaa
     3721 ggccccgaag ccctcaggcg gagcgaacaa catcgtcttc gtgcgcagcg gtagcgatat
     3781 cgtgcaggtg gacctggatg aagtgcgcta cgtgaaaagc gagagcaatt atgtgcgatt
     3841 cgtgctcgat ggacgcgatg tgctgagctt gatgaacatg aaggacctcg gtggcaaact
     3901 gcccggggcc ttcgtgcgcg tacaccgcag ctatttcgtg aacctgcgcc acgtggagaa
     3961 gctggatacc aatgacatca aggtggggcg cgatctcatc cccgtgagcg agacctaccg
     4021 acagtcgttg atcgcgcagt tggcgctgtt gtgagatggc cgacaacgca gcggtccggt
     4081 acgcaggcgg aacgatcagt gggccccggc cagccggatg ctcgagatca tgttgatcag
     4141 gtagtcggcg aatgcggtgt cgcccttgcg gttcatctcc ctgtcggtca agcttcttgc
     4201 gcgatcgcgc tggatggccc atgaactccc tggttgtacg ggatgtgctt ctggatgtcc
     4261 ggatcgtccg gaggcggctt caacccgcga tggtgatccc ggcggatcgc cgttgttccc
     4321 gcatcatcgc ccggtcgcag gccgcgaacc agtggaccac ggccgcctcg gttcccggcg
     4381 tcacgggtat gaccagcgcg tccagggcgg caaggccctt ttccaacccc agcaattgcc
     4441 agtgcggccg cacttggtgc cgggtacgcg agagcaggtc cgcatccccc gcggtgatgg
     4501 cttgaccgat ggatacgcga tgtttcgcga attcatccct gaattgctgg atcaggtcga
     4561 tcgccctggt tccgctgacg ccgtactggt cgagaagtgt ggcgattccg atggtcccat
     4621 catcgcgctc atcggtccac caggccgcgg ccgctgccag tgcgtcgcgg tccaatggct
     4681 tggtgatgcg cgcgttcatg ccggccagta ggatctccgc atcggtgtcg tctgatgcgt
     4741 gcgccgtgag tgcgagtatg ggcgtatagc ggtgcctgcc ttcgcggcgc aactggcggg
     4801 cgagttcgat gccgctgtcg ccactcccga gttccaggtc caggaggagc aggtcgaacg
     4861 tgcctgccgt acaggccgaa cgggctgacc cactgctctc cgcctcggta agtgcccagt
     4921 tccaacgggc ggcccattgc cgcatcaaca ggcgattggt ggccacatcc tcgacatgga
     4981 gcacccggag gccccgggtt tccaccgcgg tcacctgtac cgttgatccg ggatcagttg
     5041 ccgcggttgg caggagcacg gtgaaccgcg tgccccggcc cacttcgctg tccatggaaa
     5101 tgcggccgcc catcaggtcc gttacgcgcc gggtgatcgc cagaccgagt cccgcaccat
     5161 ggccctgctc gctggagctc gcgcgctcga agcgttcgaa tacgcgctct cgctcgttcg
     5221 ccgggatgcc cggccccgtg tcggccacct ggatgcgcag tgttcccggc ccatccatcc
     5281 ccacctccag ggtcacctgg cccacctcgg tgaagcgtac cgcgttgccc agcagattgt
     5341 cgacgatctg gtgcagacgt aacggatcga ggagcaaggc agggggcagg tcgtggagga
     5401 tcgtgtggat ccgaagccct ttggttcgcg cactcgggag atgcgcttgc gtcacatcgt
     5461 tcaccagtgt gcgcacgtcc acgggcacgg ggtgaagggc gagcttgccc tccccgatgc
     5521 gcgcatgcat cagcaggtcg tccaccagcc cgcgaagccg ctccgcactg cgttgaatga
     5581 tcgccatgtg ctcacggtcg gcaggtccaa ggtgcgcggt gtcgatctcg ccgcccatgc
     5641 cgatgatggc gttcagcggt gtgcgtacct catgactcat gttggccagc aattcccgac
     5701 gctggcgatc actggtctcg gcggccacgc gcgcctgttc caattcgcgc acgcgggcgt
     5761 cgatacgctc ctgcatggta cgcaggcccc gtgccatgcg gccgaactcg tcgttgcgtg
     5821 ctgtgggcag ttcctcgttg gaacccacgg catagcgctc catcaaaccg gtgaggcggt
     5881 tgagccgctc ccggaaccgg agcgcgaaca tggccgtgag cgcgatggca ccgagcgcga
     5941 tgccggcgaa cagcaacacc agttcgttgc gccggtgccg caggcttccg ggcaatccgc
     6001 tcaaggatcg ggtgatcgcc atctcgaagc gtgccttgga cgggcccaat tcgaattcgc
     6061 ggcgcccgat gagcacctcg gctccatcct ctttcggcag gacgttctcc atccgacgcg
     6121 atccacccag gtcgaaccgg aagcattgcg aggtgtcggg gtgcaggatc acttcgccat
     6181 caccgcgcgc cagcacaagg acgctgccac tgtcggcaag ggcgagcagt tcggcgaaga
     6241 gtgcgcgcat gtcggcgttg atgatcacca tgccggcac
//
LOCUS       DAOQOC010000022         6218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_4902_length_6218_cov_3.948239, whole genome shotgun sequence.
ACCESSION   DAOQOC010000022 DAOQOC010000000
VERSION     DAOQOC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4902_length_6218_cov_3.948239"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..989
                     /locus_tag="PL106_00680"
     CDS             186..989
                     /locus_tag="PL106_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31607.1"
                     /translation="MRKLMTILALGCTSNMWAQGEVIRLEPRILLDGCYVASAGLMHD
                     SLRTKQLIPEEEPFSALGYFHVGGGGGEQILPGVLNVEGPDAIVDWVVVELREAAANG
                     FRVATQSALLQRDGDVVGMDGFSAIVFDVPNDFYFLSIKHRNHLGVMTSSAYYFGPDA
                     LPLDFTALATPVWGVLGRRFVDSRALLWCGDANGNGQVKYTGNGNDRDQILSLVGGTS
                     PNVSLTGYYRQDVNMDGRVRYTGTGNDRDRILTTIGGTMPNATRTGQVP"
     gene            complement(1085..3064)
                     /locus_tag="PL106_00685"
     CDS             complement(1085..3064)
                     /locus_tag="PL106_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNA31608.1"
                     /translation="MKTRSTLLLLGIALSGIAARAQNSCSNAVPIVAGTYTIDQVNGN
                     EIPVPLCNNNDVGALHTEWYSYTAAADLSLTISTDLPQNAGRDTRFNVYGGTCGQLSC
                     VGGADDQDGNTRATATVQVSSGLTYRIAFDDRWENNGFDFELTEGPPVVTEFSFSPVA
                     INVSGSILGAADMTGDMLDDVVGVTGNNVSLAKQLAGGGFATVNIPTAMAAYPASWSM
                     AIGDLNGDGYNDLEYGNGSGVSLMLSANNGTSFTEFHVSQYVFSQRTNMVDINNDGLL
                     DGFVCHDTQANVFYMNNGDGTFDFAQGGLGETCGNYGSVWIDYDNDHDMDLFVAKCGC
                     DPVDILYRNNGDGTYTNVAPALGLADSHSSWSSAWGDYDNDGDMDLFIGSSGSPSHKL
                     LRNDDGAFTNITVGSGLDTYTATSIEWTCHDFNNDGWVDILGAGAMLMNNGDMTFTPN
                     YDTPMNGPIGDLNNDGFLDIVYGNTLYLNSGNTNKYLVVRLQGTTSNRNGIGARVVVE
                     SPLFTQIRDVKSGDGFKYASTLNAHFGLGADATVSRVTVYWPSGIIDVIEDVNVNSAM
                     HIVEGSSISTGMATGNKKEGINLFPIPATDLLQVRSENDLTNASVTVLDVAGREVMKG
                     VLRNGQLDISGLTNGVYVLRLPIEGRITQHKFVKR"
     gene            complement(3130..4230)
                     /locus_tag="PL106_00690"
     CDS             complement(3130..4230)
                     /locus_tag="PL106_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31609.1"
                     /translation="MKRIHLIVIAVAMSLAGCRKEDRPDPDPTGSVPPSSTSQVFVDL
                     AEMPYDSLSKYGFFQGPMSGMQPSEGVLPFAPINALFSDYAKKSRFVWMPPGVSATYE
                     SDSSALVFPDGAVLIKTFYYDGVQPDNGRRILETRLLFRRNGGWEFADYRWNEEMTEA
                     VYDMTGQNVPITFTDDAGTSHDVIYRIPTETECLTCHRKANAPVPIGAKPQNMNATFA
                     YADGPMNQLDKWVSQGYLNGGLPSTINTVADWTDSSLPLNDRVRGYVDINCAHCHTDG
                     RYCDYRPMRFSWTQTTDPAMLGVCVPPHQPLLPIHSHIVKPGNLEKSLLYYRINSGED
                     GIRMPLLGRTLIHEEARQLIADWIGSLNKTCE"
     gene            complement(4683..>6218)
                     /locus_tag="PL106_00695"
     CDS             complement(4683..>6218)
                     /locus_tag="PL106_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNA31610.1"
                     /translation="DFLIGEAVTYGTSSFYDFLSKANAGKKAYVCNGSACLVAGTQDR
                     VHHELAKHFKAEEIGHMCCLGRCHENSAFNVGGLNYSGKAIDRIGDIAGGRTRDGMDA
                     HFVGTSMERPILTAPMPPLEEFYALWERVLKGDPLDVLNGIKVATIRGRGGAGFPMGF
                     KLQACRDAKDTTGNGTPRKYIVCNADEGDPGAYSDRYLLEQRPHLVLLGMMLAGFCVG
                     ADTGVLYIRAEYPEAVEIVKRAVADIEARGWIGNDIQGSGFNWRFKVIKAAGAYVCGE
                     ETALLNSIEGKRGEVRTRPPYPAQQGLFNRPTVVNNVETLACVPWVVKNGGAAFAKLG
                     SEKSNGTKLVCLDSGFKRPGLYEVECGTPLSTVIDDLGQGFARPTKALHIGGIVPMQK
                     IGDLSIDFESFQREGFLLGHASVLSIPADFPMVAYLEHLFEFTAMESCGKCFPCRIGS
                     VRGKELITGAREGRRIDRALFNDLIETMEIGSLCALGGGLPLGVKNALAYFREELDVY
                     FAT"
BASE COUNT         1095 a         1866 c         1925 g         1332 t
ORIGIN      
        1 ggcggtggcg gtgcgcgcgg taggggcgga ggatccgtgc ttggcgttcg gcgtgttcat
       61 ggtgtatccg atgcgatccg gtgttggcgt ccggggccgt ggtcgcacga aaataatggt
      121 ggatcgtgga tggagctacc ttcggatccc tgcgaccccg atgaagccct cgcagggatc
      181 tgttcatgag gaagctgatg accatactcg ccttgggttg caccagcaac atgtgggcgc
      241 aaggcgaggt catccgcctc gaaccccgga tcctgctcga tgggtgttat gtcgcttcgg
      301 ccggtttgat gcacgactcc ttgcgtacga agcagctgat ccccgaagag gaaccttttt
      361 cggcactcgg atacttccat gtgggcggcg gcgggggtga gcagatcctg cccggtgtgc
      421 tgaacgtcga aggccctgat gcgatcgtgg attgggtggt ggtcgagctt cgggaggccg
      481 cggccaacgg ctttcgcgtg gccacacaga gcgcgctgct tcagcgcgat ggtgatgtgg
      541 tgggcatgga cgggttctcg gccatcgtgt ttgatgtgcc caacgacttc tacttccttt
      601 cgatcaagca caggaaccac ctcggggtga tgacctcctc cgcgtactac ttcggcccgg
      661 acgctttgcc gctcgatttc acggcgcttg ccacaccggt atggggtgtg ctcgggcgca
      721 gattcgttga ttcgagggca ctgctctggt gcggcgatgc gaacggcaac ggccaggtga
      781 aatacaccgg caacggcaac gatcgcgatc agatcctttc cctggtgggc ggaacttcgc
      841 ccaacgtcag cctgaccggg tactaccgtc aggatgtgaa tatggacggg cgggtgcgtt
      901 acaccggtac gggcaacgac agggatcgga tcctcaccac cattggcgga acgatgccca
      961 atgcgacgcg gaccggacag gtgccatgag caaatggtac tgtcccaggc gtgggtgccg
     1021 aacacgagcg ccgatcatcc gttgatgatc ggcgctcata gcttggtatg gcgaaaggcc
     1081 ccggtcagcg tttgacgaac ttgtgctgcg tgatgcgccc ttcgatcgga aggcgcagca
     1141 cgtacacgcc gttcgtaagc ccggagatgt cgagctggcc gttgcgcagt acgcctttca
     1201 tcacctcgcg accggccacg tcgagcacgg taacggaagc gttcgtcaga tcgttctcgg
     1261 accggacctg aaggaggtcg gtcgccggta tggggaacag gttgatcccc tccttcttgt
     1321 tgccggtggc catgccggtg ctgatggagg acccctccac gatgtgcatg gcggaattga
     1381 cgttcacgtc ctcgatcacg tcaatgatgc cacttggcca gtatacggtc acgcggctca
     1441 cggtggcgtc ggcacccagg ccgaagtggg cgttcagcgt gctggcgtat ttgaatccgt
     1501 cgccgctctt cacatcgcgg atctgggtga acaagggact ctccaccacc acacgtgcgc
     1561 cgatgccgtt acggttgctg gtggtgcctt gcagccggac cacgaggtac ttgttcgtgt
     1621 tgccgctgtt gaggtacagc gtgttcccgt acacgatgtc caggaagccg tcgttgttga
     1681 ggtccccgat gggcccgttc atcggcgtgt cgtagttggg ggtgaaggtc atatccccgt
     1741 tgttcatgag catggccccg gcacccagga tgtccaccca tccgtcgttg ttgaagtcgt
     1801 ggcaggtcca ttcgatggag gtcgcggtgt acgtgtccag gccggatccc acggtgatgt
     1861 tggtgaaggc gccgtcgtcg ttgcgcaaca gtttgtggct cgggctgccg ctcgatccga
     1921 tgaacaggtc catgtcgccg tcgttgtcgt aatcgcccca ggcactgctc caactcgagt
     1981 gggaatcggc caggcccagc gccggcgcca cattggtgta tgtgccgtcg ccgttgttgc
     2041 ggtacaggat atccaccggg tcgcacccgc acttggccac gaacaggtcc atgtcgtggt
     2101 cgttgtcgta gtcgatccat acggagccgt agttgccaca ggtctcgccc aggccacctt
     2161 gggcgaaatc gaaggtgccg tcgccgttgt tcatatagaa cacgttcgcc tgcgtgtcat
     2221 ggcaaacgaa gccgtccaat agaccatcgt tgttgatgtc caccatgttg gtccgctggc
     2281 tgaatacgta ctggctcacg tggaattcgg tgaaggaggt gccgttgttg gccgagagca
     2341 tcaggctcac gccactgccg ttcccgtact ccaggtcgtt gtacccgtcc ccgttcagat
     2401 caccgatggc catgctccaa ctggccggat acgcggccat ggccgtcggg atgttcaccg
     2461 ttgcgaagcc gccgcccgcg agctgtttgg ccaggcttac gttgttgccc gtcacgccga
     2521 ccacatcgtc cagcatgtcg cccgtcatgt cggccgcacc aaggatgctg ccgctcacat
     2581 tgatcgccac cgggctgaag ctgaattcgg tgaccaccgg cggaccctcg gtcaactcaa
     2641 aatcgaatcc gttgttctcc cagcggtcat cgaacgctat gcggtacgtg aggcccgatg
     2701 atacctggac cgtggccgtg gcgcgcgtgt tgccgtcctg atcatcggcg ccgccaacgc
     2761 aactcagctg accgcacgtt ccgccgtaca cattgaaacg cgtgtcgcgt ccggcgttct
     2821 ggggcagatc ggtgctgatg gtcagggaga gatccgccgc tgcggtatag ctgtaccatt
     2881 cggtgtgcag cgcccccaca tcgttgttgt tgcacagggg cacggggatc tcattgccgt
     2941 tcacctgatc gatggtgtac gtgccggcga cgatgggcac tgcgttcgag cagctgttct
     3001 gcgcgcgtgc ggcgatgccc gacagcgcta ttccgagaag caggagtgtg gagcgtgtct
     3061 tcatgtgtgt acgtccgggt tcggcggatg ctggtttttg gtgttcttct gagtggttcc
     3121 cgtaaggggt cattcacagg ttttgttgag gctcccgatc cagtcggcga tgagttgacg
     3181 cgcctcctcg tggatcaatg ttcggccgag cagcggcatg cggatgccgt cctccccgga
     3241 gttgatccga tagtaaagta acgacttttc caggttgccc ggctttacga tgtgcgagtg
     3301 aatgggcaaa agaggctggt ggggcggcac acaaacgccc agcatcgccg ggtccgtggt
     3361 ctgcgtccag ctgaagcgca tgggccggta gtcgcagtac cggccatcgg tatggcagtg
     3421 cgcgcagttg atgtccacat agccccgcac ccgatcgttc aagggcagcg aactgtccgt
     3481 ccagtccgcc acggtattga tcgtggaagg caggcctccg ttcaggtatc cctgcgacac
     3541 ccatttgtcc aactggttca tgggaccatc tgcgtatgcg aacgtggcgt tcatgttctg
     3601 cggtttcgca ccgatgggca ccggagcgtt cgccttgcga tggcaggtca ggcattccgt
     3661 ttccgtgggt atccggtaga tgacgtcgtg cgaggtcccg gcgtcgtccg taaaggtgat
     3721 cggtacgttc tgaccggtca tgtcatatac cgcttcggtc atctcctcgt tccatctgta
     3781 gtcggcgaac tcccacccgc cgttgcgacg gaagagcagg cgcgtctcca ggatgcgccg
     3841 accattgtcc ggttgcacgc cgtcgtaata gaatgtcttg atcagcacag caccatcggg
     3901 aaagacgagc gcggaactgt cactctcgta ggtggcggac acccccggtg gcatccatac
     3961 gaaccggctc ttcttcgcgt agtcgctgaa caacgcattg atcggggcga aaggcagcac
     4021 accctccgat ggctgcatcc ctgacatcgg accttggaag aagccgtact tgctgagcga
     4081 atcgtagggc atctcggcga gatcgacgaa gacctgggag gtggatgagg gtggaacgct
     4141 tccggtggga tcggggtccg gccggtcttc cttcctgcag ccggcaagcg acatcgccac
     4201 cgcgatcacg atgaggtgga tcctcttcat aagggtactg ctccgttcgg tggatgccgg
     4261 ggtcctgacg gaagtggacc gcatgcgcgt tgcccggtgt cgcgttccac gcccgtatgt
     4321 ccggtgttgt cgccgggccc ttccgtccgc cttgtcacgg gcgcgcggca tgccgtcact
     4381 gatcaaggta ttatttgttt tttcaggggg cgatggggtg cggatcccgt tcgtacgata
     4441 tcaacaggtg tcggttgaag gatctttggt ggaacatgat acgcgtatgc gatcacgcat
     4501 ttatgtttga cccaaacctt aacaccatgg caaagaaggc aaccaagaag aaagcagcga
     4561 aaaaggccgc tcccaagaag aaggccgcga aaaaggccgc gaagaagaag tagttttctt
     4621 cttgaacctt gtggaagccc cgacatgtcg gggcttcccc gttcctgggc cttccttccc
     4681 tgtcatgtgg cgaagtacac gtcgagttcc tcgcggaaat acgccagtgc gttcttcacg
     4741 ccgagaggca gccctccccc gagcgcgcac aatgacccga tctccattgt ttcgatcaga
     4801 tcgttgaaca acgcacgatc gatccggcgt ccttcacgtg cgccggtgat cagctccttg
     4861 ccgcgcaccg atccgatccg gcaggggaag cacttgccgc aactctccat cgcggtgaac
     4921 tcgaacaggt gttccaggta ggccaccatc gggaagtcgg ccggtatgga cagcacgctc
     4981 gcatggccga gcaggaatcc ttcccgctgg aacgattcga aatcgatcga caggtccccg
     5041 atcttctgca tgggcacgat gccgccgatg tgcagtgctt tggtcggccg cgcgaagccc
     5101 tggcccagat catcgatcac ggtgctgagt ggtgtgccac attccacctc gtacaggccg
     5161 gggcgtttga atccgctgtc caggcaaacg agtttggtac cgttgctttt ctcggaaccg
     5221 agcttggcga aggccgcacc tccgttcttc accacccagg gcacgcaggc cagcgtctcc
     5281 acgttgttca ccaccgtggg ccggttgaac aaaccttgtt gtgcggggta gggcgggcgt
     5341 gtacgcactt cgccccgctt gccttcgatg ctgttcaaca gcgccgtctc ttcgccgcac
     5401 acgtatgccc cggcggcttt gatcaccttg aagcgccaat tgaacccact gccctggatg
     5461 tcgttcccga tccaaccgcg tgcctcgatg tccgccaccg cgcgcttcac gatctcgacg
     5521 gcttcgggat attccgcacg gatgtacagt actcccgtat cggcaccaac acagaagccg
     5581 gcgagcatca tgccgagcag taccaggtgc gggcgttgct ccaacaggta ccgatcgctg
     5641 tacgcaccgg ggtcgccctc atccgcgttg cacacgatgt acttgcgcgg tgtgccgttg
     5701 ccggtggtgt ccttcgcatc gcggcaggcc tgcagcttga aacccatcgg gaagcccgca
     5761 cctccgcgcc cacggatcgt agcgaccttg atcccgttca gtacatcgag tggatccccc
     5821 ttcagtaccc gctcccacaa cgcgtagaat tcttcgagcg gcggcatcgg tgcagtgagg
     5881 atgggacgct ccatggaggt gcccacgaag tgcgcatcca ttccatcgcg cgtccttccg
     5941 ccagcgatgt caccgatccg gtcgatcgct tttcccgaat agttcaggcc gcccacgttg
     6001 aatgcgctgt tctcatggca ccggcccagg caacacatgt ggccgatctc ctccgccttg
     6061 aagtgcttcg ccagttcatg gtgcacgcga tcctgtgtgc ccgccaccag gcacgcactg
     6121 ccgttgcaca cgtacgcctt cttgccggcg ttcgctttgc tgaggaagtc gtagaacgaa
     6181 ctggtgccgt aggtcaccgc ctcgccgatc aggaagtc
//
LOCUS       DAOQOC010000023         6091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5056_length_6091_cov_3.132869, whole genome shotgun sequence.
ACCESSION   DAOQOC010000023 DAOQOC010000000
VERSION     DAOQOC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5056_length_6091_cov_3.132869"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2292)
                     /locus_tag="PL106_00700"
     CDS             complement(<1..2292)
                     /locus_tag="PL106_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31611.1"
                     /translation="MKKQYSYLKSAMFALRGTAAVMSALAMVQSHAQLNIAAANTAYN
                     INFDGTLAGVGNGVWAGNGFQPAPTAGRLDSDAWQVTDWSNGNLPYGGTRITSGTDYR
                     RGVAVAPIAVGGMYAFDDGVGGTLNGRALGFQPGSSDWAPGSLTLRVQNNTGSTLTAF
                     DIAYNVYYRDDQGRSSDFNLYVSTDNVSYTHISSQDVVSPVGPAGIAWVANARSVTVN
                     GYYVPNGQYFYIRWRGNDVAGSGSRDEFALDDISVTGRVYTLVRLTSSSSNADEDDGT
                     ATLTASIVNPHPTNPTTVDVALSSGDAARIDNYTTQTLVFPGGSIANQNLTINITDNG
                     ACDGDVVETFMLQNVTGGITPGIGSPDTHIMTIDDDETAPVSHAQNFDGGPGDDWPIT
                     LGGGNQSANTGAGDTPASQRILSASQSWQVNNGTATLELGAVDVQDWSSITLSAHLTS
                     TSLNATNGADVPDSVAFYVDLDGAGFPLDPDISVSGNSNRRYGYGASGIASTTAGTPV
                     TYICNFGGNGIDYATVNITIPNGTNTVALRVIGKNNDANEVWNLDNIQVSGTLCSPIY
                     YSRSNGSETTGTWSTSRTGAPAPGVVTFTKNATMVIQATHTVTSTSNATIDLRDLDVE
                     TGGTLDLAGNGTVSINGPSFNVDGTLTATDDAFNILGDALTTISGATSSIDVEDLTLD
                     GFGAQVTTTNLRIFGTLQLDNGNFDANGKNVVLASTATGTGRLGPVDPAASYTGSIRM
                     ERYIPAGVTDWRLLCSPVQGRTVY"
     gene            complement(2438..3268)
                     /locus_tag="PL106_00705"
     CDS             complement(2438..3268)
                     /locus_tag="PL106_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNA31612.1"
                     /translation="MAGNTTFGANIKLLRTRRGRSQEETAIALDVKRSSWSGYENGSA
                     EPNLDLLVRISAYFKISVDKLLKHDLTLLSESQLGILERGGDVDMDGRRLRVLATTVS
                     KDNEENIELVPEKARAGYALGYADPEYISVLPTFQMPFLGRDRKYRTFQISGDSMPPV
                     GNGSWVTGEYVQNWQMIRDGQPYVVVTKDDGIVFKVVYNKLKEKGTLLLCSTNPVYPP
                     YEVEVGNVLEIWKFVHFISQELPEPNLSRDELARSVMVLQKEVGQLRLSIEQQGRLAL
                     "
     gene            3363..>6091
                     /gene="dnaE"
                     /locus_tag="PL106_00710"
     CDS             3363..>6091
                     /gene="dnaE"
                     /locus_tag="PL106_00710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNA31613.1"
                     /translation="MFLNCHSWFSFKYGVLKPEALLEEAAKAGVRTLALTDIHCSAGI
                     PDFVRDAPRYGVRPVAGIEFRKGPRLLYIGIAMNNNGLQQLNELLSPHLLDDEAIPER
                     APALTDVFFIHPFSHAPQQLRPNERVGIRPSDLTRLPFSPWVKRQHELVALMPVTFRR
                     CERTSVRGCEGERVMEPGTLAPSHSLTSSPSKKDYNTHRLLRTVAKNTVVSMLPKEEL
                     AQADECFRSEEEVRRIYNDHPQLIANAERVLEQCSIVFDGSDKTRVAFTNDVRADREK
                     LHRDTREGLRYRYPEVTPKVLARMHHELDVIERMGFISYFLINQDIVNYARSRGFFHV
                     GRGSGANSLVAYCLRITDVDPMELDLYFERFINPARKKPPDFDIDFSWKDRDEVYRYV
                     FNKYNSTDGRAGGIHAAQIATYTTFQWRGAIRELGKAVGLPPAEIDALSEGDHGYYSR
                     GRPSAHAKSNEVDKVAQAVVRYGQQLIGMPHHLGIHAGGIVITEKPVTHFSALFRPPK
                     GFPVTQISMLECEDMGLHKFDLLSQRGLGHIRDAVELVQASSHKDQASSLNAGALEAC
                     DLKLEPCDFRAAVDIHDIPRFKQDPAIKELLRKGDTIGCFYVESPAMRMLLKKLQVDD
                     YLGLVAASSIIRPGVAESGMMREYLLRHNDPERMKQAIPRLLEIMPETYGVMVYQEDV
                     IKVVHLYGGLDLEESDTVRRGMNLRYRDRPEFKVVEKKFFENCKAFGYPPGEAEEVWR
                     QIQSFASFSFAKGHSASYAVESYQSLYLKAHHPLEFLVGVANNFGGFYRLEFYLHEAK
                     RAGAVIEAPCVNASEELCALVQVEQVNGQWRIANGASLHSPTPIRHSPSDPRIYLGLS
                     NIKSLENESVELILHERRRKGPFLHLEDLLKRVPLSLEQARILIRVGALRFTG"
BASE COUNT         1201 a         1883 c         1796 g         1211 t
ORIGIN      
        1 gtacacggtc cgaccttgta cgggggagca caggaggcgc cagtcggtga cacccgctgg
       61 aatgtaccgt tccatgcgta tggatcccgt gtagctcgcc gccgggtcca ccgggccaag
      121 acgtccagtg cccgttgccg tgctggccag caccacgttc ttcccgttcg cgtcgaagtt
      181 cccgttgtcc agttggagcg tgccgaagat cctcaagttg gtcgtggtca cctgggcccc
      241 gaagccatca agcgtcaggt cttccacatc gatcgacgaa gtggctccgg aaatggtcgt
      301 gagcgcatcg cccaggatgt tgaacgcgtc atccgttgcg gtcaatgttc cgtccacgtt
      361 gaaagagggg ccattgatcg acaccgtacc gttcccggca aggtccagcg ttccgcccgt
      421 ctccacatcc agatcgcgca gatcgatggt cgcgttggac gtgctggtca ccgtgtgggt
      481 ggcctggatc accatggtgg cgttcttggt gaaggtcacc acccccggcg cgggtgcgcc
      541 cgtgcgcgag gtggaccatg tgccggtcgt ctcgcttccg ttggagcggc tgtaatagat
      601 gggggagcaa agcgttccag agacctggat attgtccagg ttccacactt cgttcgcatc
      661 gttgttcttc ccgatgacgc gcaacgcgac cgtattggtg ccgttcggta tggtgatgtt
      721 caccgttgcg taatcgatgc cgttcccacc gaaattgcag atataggtca ccggcgtccc
      781 agccgtggtg ctcgcgatcc cggaggcccc gtaaccgtaa cgacggttgc tgttgcccga
      841 aacgctgatg tccggatcca atgggaaacc cgcaccatcg agatccacat agaacgcaac
      901 ggagtcgggc acatcggcac cgttcgtggc gttcaacgag gtgctggtca ggtgggcact
      961 caaagtgatg gacgaccagt cctgcacatc gaccgctccc agttccaacg ttgccgtacc
     1021 gttgttcacc tgccagctct gcgaggcgga caggatccgt tggctcgcgg gggtatcccc
     1081 ggcgccggta ttcgcacttt gattccctcc tcccaaggtg atcggccaat catcgcccgg
     1141 gccaccatca aagttctggg cgtgggatac cggtgccgtc tcatcgtcat caatggtcat
     1201 gatgtgcgta tcgggacttc cgatgcccgg tgttatgccc ccggtcacgt tctgcaacat
     1261 gaaggtctca acgacatcgc catcacacgc accattgtcc gtaatgttga tcgtcaggtt
     1321 ctggttggcg atgcttcccc cggggaagac aagggtctgc gtggtataat tatcgatccg
     1381 ggccgcatcg ccgctgctca gtgcgacatc gaccgtggtg gggttcgtcg gatggggatt
     1441 cacgatggat gccgtcaggg tcgcggtacc atcgtcctca tcggcattgg acgaggatga
     1501 ggtgagccgt accagggtgt atacgcgacc cgtgacgctg atatcgtcca gggcgaactc
     1561 atcccggctg cccgaccccg ccacgtcgtt gccgcgccag cggatataaa aatactgacc
     1621 gttggggaca taatatccgt tcaccgtcac ggagcgggca ttggccaccc acgcgatgcc
     1681 ggcaggtccg accggcgaaa ccacatcctg cgaggagata tgggtatacg agacgttgtc
     1741 cgtggaaacg tacaggttga agtcactgga ccgcccctgg tcatcgcggt agtacacgtt
     1801 gtacgcgata tcgaacgcgg tgagcgtgct tcccgtgttg ttctgtacgc ggagtgtaag
     1861 cgaacccgga gcccaatcac tgctgccagg ttggaagccc aacgcccttc cgttcaaggt
     1921 tccgccaact ccatcatcga acgcgtacat gccacctacc gcgatcggtg ccacggccac
     1981 tccacgccga taatcagtac ccgaggtgat gcgggtccct ccgtaaggaa ggttcccgtt
     2041 gctccaatcg gtcacctgcc atgcatcgct gtccaaccgt ccggccgtag gcgcgggctg
     2101 aaacccatta ccagcccaca caccatttcc gacaccggcc agcgtaccat caaaattgat
     2161 gttgtacgcc gtgttagcgg cagcaatgtt caactgtgcg tgcgactgga ccatggccaa
     2221 ggcggacatc acggccgcag ttccccgcag ggcgaacatg gctgacttga ggtacgagta
     2281 ctgcttcttc atctgaggat cggattcgcg gccaaggatc accgcagttc aggggtttcg
     2341 tagagcgttc aaacatagca aggacgcgcc cttcgccaag gctggcccaa ggcttatgtt
     2401 ttccacaacg gatcgtgatc gctccgggtc ttgaaactca caatgccagc ctcccctgct
     2461 gctcaatgct cagtcgaagc tgccccacct ctttctgcag taccatcacg ctgcgggcca
     2521 gctcgtcgcg ggagaggttc ggctcgggca attcctgact gatgaagtgt acgaacttcc
     2581 agatctccag cacgttgccc acttccacct cgtagggcgg atacactgga ttggtgctgc
     2641 acagcagcag ggtgcccttc tccttcagtt tgttgtacac caccttgaaa acgatgccgt
     2701 cgtccttggt gaccacaacg taaggctggc catctctgat catctgccag ttctgcacgt
     2761 actccccggt gacccacgag ccgttgccca ccggcggcat gctgtcccca ctgatctgaa
     2821 aggtgcggta cttgcggtcg cgccccagga agggcatctg gaaggtgggc agcacgctga
     2881 tgtactccgg atcggcgtaa cccagggcat aaccggcgcg ggccttctcg ggcaccagtt
     2941 cgatgttctc ctcgttgtcc ttgctcaccg tggtggccag cacccgcagg cggcggccgt
     3001 ccatgtccac gtctccgccc cgctccagga tgccgagctg gctttcgctc aacagcgtga
     3061 ggtcgtgctt gagcagcttg tccacgctga tcttgaagta cgcgctgatg cgcaccagca
     3121 gatccaggtt gggctcggcg ctgccgttct cgtagccgct ccagctcgat cgcttcacgt
     3181 cgagggcgat agcggtctct tcctggctgc ggccccggcg ggtgcgcagc aatttgatgt
     3241 tcgctccgaa ggtggtgttg cctgccatgg cttcttgact aatttaccga cgacagaatg
     3301 acggtttccg cgtcaaatgt atcgccgctt tccaattttc caaatcggcc ctcttcccga
     3361 ccatgttcct caactgccac tcctggttca gcttcaagta tggcgtactg aaacccgaag
     3421 ccttgctcga agaagcggcc aaggccggcg tgcgcaccct cgccctcacc gacatccact
     3481 gctcggcggg catccccgac ttcgtgcgcg atgcgccgcg ctacggcgta cggcccgtgg
     3541 cgggcatcga attccgaaag ggaccgaggc tcctgtacat cggtatcgcg atgaacaaca
     3601 acggcttgca gcaactgaac gaactgctga gcccgcacct gctggatgat gaggcgatcc
     3661 cggagcgggc tcccgccctc accgatgtct ttttcatcca tccgttcagc cacgcgccac
     3721 agcaactgcg ccccaacgag cgggtgggca tcaggccctc ggatctcacg cgtttaccgt
     3781 tctccccctg ggtgaagcgg caacatgaac tcgtggcgtt gatgccggtg acattccgga
     3841 gatgcgagcg tacgagcgtg cgagggtgcg aaggtgagag agtcatggag cccggcaccc
     3901 tcgcaccctc tcactctctc acttcttcac ccagcaagaa agactacaac acgcaccgcc
     3961 tgctgcgcac cgtggccaag aacacggtgg tgagcatgtt gccgaaggaa gagttggcgc
     4021 aagccgacga atgtttccgc agcgaagagg aggtgcgtcg catctataat gatcatccgc
     4081 agctcatcgc caacgccgaa cgtgtgttgg agcaatgcag catcgtcttc gacggcagcg
     4141 acaagacgcg cgtggccttc accaacgatg tgcgcgccga ccgcgaaaag ctgcaccgcg
     4201 acacgcgcga aggcctgcgg taccgctacc cggaggtaac gcccaaggtg ctggcccgca
     4261 tgcaccacga gttggatgtg atcgagcgca tgggcttcat cagctacttc ctcatcaacc
     4321 aggacattgt caactacgcg cgcagccggg gcttcttcca cgtggggcgt ggcagcggcg
     4381 ccaacagcct ggtggcctat tgcctgcgca tcaccgatgt ggaccccatg gaactcgacc
     4441 tctacttcga gcgcttcatc aaccccgcac gcaagaagcc gcccgatttc gacatcgact
     4501 tcagttggaa ggaccgcgac gaggtgtacc ggtatgtctt taataagtac aactccacgg
     4561 atggaagagc gggcggaatc cacgccgcgc agatcgccac ctacaccacc ttccaatggc
     4621 gcggtgccat acgcgagctg ggcaaggccg tggggcttcc accagcggag atcgatgcgc
     4681 tatccgaagg cgatcacggc tactactcgc gcggaagacc cagcgcacac gcgaagagca
     4741 acgaggtgga caaggtggcg caggcggtgg tccgctacgg gcaacagctc atcggcatgc
     4801 cgcatcacct gggcatccac gccggcggta tcgtgatcac ggagaagccg gtgacgcact
     4861 tctcggcact gttccgtccg cctaagggct ttcccgtaac gcagatcagc atgctcgaat
     4921 gcgaggacat gggcctgcac aagttcgacc tgctgagcca acgcggcctg gggcatatac
     4981 gcgatgcggt ggagctcgtt caagcttcaa gtcacaagga tcaagcctca agtctcaacg
     5041 ccggcgcgct tgaagcttgt gatctgaaac ttgagccttg tgacttccgc gcagcggtgg
     5101 acatccacga cataccgcgt ttcaagcagg atcccgcgat caaggaactg ctgcgcaagg
     5161 gcgataccat cggctgcttc tatgtggaaa gccccgccat gcgcatgctg ctgaagaaac
     5221 tgcaggtgga tgattacctg ggactggtgg cggcgagcag catcatacga ccgggcgtgg
     5281 ccgagagcgg catgatgcgc gagtacctgc tgcgccacaa cgatccggaa cgcatgaagc
     5341 aggccattcc gcgtttgttg gagatcatgc ccgagaccta cggtgtgatg gtataccagg
     5401 aggatgtgat caaagtggta cacctctacg gcggcctgga cctggaagag tcggacaccg
     5461 tgcggcgcgg catgaacctg cgctaccgcg accggccgga gttcaaggtg gtggagaaga
     5521 aattcttcga gaactgcaag gcgttcggct atccgcccgg cgaggctgaa gaggtgtggc
     5581 ggcagatcca gagcttcgcg agcttcagct tcgccaaggg ccacagcgcc agctacgccg
     5641 tggagagcta ccagagcctc tacctgaagg cgcaccatcc gctggaattc ctcgtgggcg
     5701 tggcgaacaa tttcggcggc ttctatcggc tggaattcta cctgcatgaa gcgaaacgcg
     5761 cgggtgcggt gatcgaggcg ccgtgcgtga atgccagtga ggagttgtgc gcgttggtgc
     5821 aggtcgagca ggtgaatggt caatggcgaa tagcgaatgg ggcatctctc cattcgccaa
     5881 ctcccattcg ccattcacct tctgatccac gtatatacct cggcctctcc aacatcaaaa
     5941 gcctcgagaa tgaaagcgtt gaactgatcc tgcacgaacg ccgccgcaag ggaccattcc
     6001 ttcatctgga ggatcttttg aaacgagtcc cgctctcgct ggaacaagcg cgcatcctga
     6061 tccgcgtggg cgccttgcgc ttcaccggaa a
//
LOCUS       DAOQOC010000024         6065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5096_length_6065_cov_3.011814, whole genome shotgun sequence.
ACCESSION   DAOQOC010000024 DAOQOC010000000
VERSION     DAOQOC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5096_length_6065_cov_3.011814"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /gene="carA"
                     /locus_tag="PL106_00715"
     CDS             <1..1063
                     /gene="carA"
                     /locus_tag="PL106_00715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNA31614.1"
                     /translation="GEICFNTGMTGYQEIFSDPSYTGQIMVMATPHIGNYGVKEGEEE
                     SRAFTIAGLVVKKYSEVWSRPGGTGPLDDALKAAGITGISDIDTRKLVRHIRDHGAQN
                     AIISSSVLDLDRLRSLLDGAPDMAGRELSSSVSTRTAYSIGPADAPHRVALVDFGVKR
                     NIERCLLERDCHVRVFPMTTTVEEMLEFRPNGFLLSNGPGDPGAMPASVALVKAVVDT
                     GLPVFGICLGHQLLAESQGIGTEKMHHGHRGINHPIKNLVNGRDEVTSQNHGFVANRE
                     QTERNADVGITHVHLNDNSIAGIRLKGKPVFSVQYHPEAGPGPYDSRYLFDQFIQLME
                     GPRPSAPATSAQTKTTTRA"
     gene            1060..2346
                     /gene="eno"
                     /locus_tag="PL106_00720"
     CDS             1060..2346
                     /gene="eno"
                     /locus_tag="PL106_00720"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNA31615.1"
                     /translation="MSLIAKIQAREILDSRGNPTVEVDVFTDSGHMGRAAVPSGASTG
                     AHEAAELRDGDKKRYLGKGVLKAVEHVNETLNNELHGALVTEQAMIDKAMVGLDGTRN
                     KGNLGANAILGVSLAVAKAAASEAGLPLYRYVGGSNARTLPVPMMNILNGGAHADNKV
                     DIQEFMIMPVGADSFAQGLRMGAETFHHLKAVLKSKNLGTNVGDEGGFAPDLKSNEDA
                     LKLVMQAIEKAGYEPGEDIVIALDCASTEFYDAKKKRYVLESTGDSLTSDDMAALLSG
                     WCKKYPIVSIEDGMAEDDWDGWKKLTEAIGGKVQLVGDDLFVTNTERLAKGIQGGIAN
                     SILVKVNQIGTLTETLEAVDLAQRNSYTAVMSHRSGETEDSTIADLAVAVNCGMIKTG
                     SASRSDRIAKYNQLLRIEEQLGKSAIWPGRRLRYVG"
     gene            complement(2362..4761)
                     /locus_tag="PL106_00725"
     CDS             complement(2362..4761)
                     /locus_tag="PL106_00725"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNA31616.1"
                     /translation="MTRPSPGRTTVAAITLLSTLPLAAQTGSLTRSDSLDLLHTRIEL
                     DLRETGSQLIHGSAHIRFVPRVEGITTLPLDLLDLTVDSVRQTGGDPLNFMHTGEELL
                     VDLGVAHGPADTLEVVIHYGGNPTVDASGWGGFYTTSAYQYNLGVAFDAVPHSYGRAW
                     FPCFDNFVERCTFEFQVRVNGGRTVFANGTLIADVDEGDGQRLFHWRIEEPIPSYLAS
                     VSATNYVAVRDSFPSISGALVPVALAAKPNDTTDLKNSFEHLHDAFDTFERWFGPYRW
                     ERVGYCLTTQGAMEHPTNISYPGSIADGTLQYEDVMAHELAHHWFGDLITCRHAGEMY
                     INEGYAEFLSYLFLEDVYGRDRYMRTVRNNHHDMVSTAHLQDEGWWALNNVPQEHTYG
                     EHSYRKGADLAHTLRGYLGDSLFASGYTRVLTNNAYSDMGTEELRDSLSVATGTDLTD
                     YFADWIMQPGWAAFEVDSFTTAQDGGAWPTTLYVQQKLRHADHFYHNVPVTITFEDAD
                     GTRWTDPERTILGNEHSTAVSTPPFQPVAAFLNAEQLLALATTITEDTVTNTGTKNLL
                     LADVSLITSVLPSAAPMRVEEFWTAADATGEGSGSVASPDRWWRITGRPAQGTAMDLR
                     LTLDGRPTSGTCFDPGLVADIGGSGFQEDSLLMLYRPNAHFPWSIHADTEINTIGSDA
                     SDGYARLTAHGMTPGDWCIGLRQNATGVATSGDALRGWKLYPNPASEHLVMEAPRAVR
                     GLDARIIVRDTLGRTLIDRPASANITRLNITGIAAQSVFVSARIQGIGEVMLGQVNIT
                     R"
     gene            4885..>6065
                     /locus_tag="PL106_00730"
     CDS             4885..>6065
                     /locus_tag="PL106_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31617.1"
                     /translation="MKPSTELFDLIRSLTKSEKRFFKLHSTLQSGTKNYMRIFDAIDR
                     QHVYDEDALRRQFEGENFVKHLPSEKNHLYKLVLKSLRAFHAEATVSGTLKIASKNIE
                     ILFAKALYEEASRTLQRAKRVAKENEKFYYWYELLNQEKQLLEEDYASGRFSRDLEDL
                     VAEEREVLAKLGNLAEYHVLYSRINFVFRSGGHARTDEERAMVEAISEHPLIKGKNTA
                     LSHRAATICYYTQGFCQWAAGDTERSLEKFLRVKKILDDHPHLRADLPKRYVRALEYI
                     IRCRVELGAFKEARDTIRLMRQSAIQDGFRGIDIAVQVFHASYLGELRMLDRMGEHMK
                     AVALTTDILHGIEEHGARLHKEQLLEFWSGLAIAHFGAGQVNKALFWLNKVLNDPEPQ
                     LR"
BASE COUNT         1199 a         1835 c         1944 g         1087 t
ORIGIN      
        1 cggggagatc tgcttcaata ccggcatgac cggttaccag gagatcttct ccgatcccag
       61 ttacaccggc cagatcatgg tgatggccac gccgcacatc ggcaactacg gcgtgaagga
      121 aggcgaggag gaaagccgtg ccttcaccat cgccgggctg gtcgtgaaga agtacagcga
      181 ggtctggagc cgcccgggcg gtaccggccc attggacgat gccctcaaag ccgccgggat
      241 cacgggtatc agtgatatcg atacgcgcaa actggtacga cacatccgcg atcacggtgc
      301 acagaacgcc atcatcagca gttcggtcct ggaccttgac cggctgaggt ccctcctgga
      361 cggggccccc gacatggcgg gccgggaact cagctcgtcg gtatccacgc gcaccgcgta
      421 ctccatcggc ccggccgatg cgccgcaccg tgtggccctc gtggatttcg gtgtgaagcg
      481 gaacatcgag cgctgcctgc ttgagcgtga ctgccacgtg cgcgtcttcc cgatgaccac
      541 cacggtggaa gagatgctgg agttccggcc caacgggttc ctcctgagca acggcccggg
      601 cgaccccggt gccatgcccg ccagcgttgc cctggtgaaa gccgttgtag atactggttt
      661 accggtattc ggtatctgcc tgggccatca actgctggcg gaaagccagg gcatcggtac
      721 ggagaagatg caccacggcc atcgcggcat caatcatccg atcaagaacc tggtcaatgg
      781 ccgcgatgag gtcaccagcc agaaccacgg cttcgttgcc aaccgggaac agaccgaaag
      841 gaacgcggac gtggggatca cccatgttca cctgaatgac aacagcatcg cggggatccg
      901 cctgaagggg aaacccgtat tcagcgtgca gtaccacccc gaagccggcc ccggtccgta
      961 cgactcacgc tacctgttcg accagttcat ccaactcatg gaaggtcctc ggcccagcgc
     1021 cccggccact tccgcccaaa ccaagacgac aacccgagca tgagcctcat cgccaagatc
     1081 caggcccgcg agatcctcga ttcgcgtggc aaccccaccg tcgaggtgga tgtgttcacc
     1141 gatagcggcc acatgggccg tgcagcggtg cccagcggtg ccagcacagg tgcgcacgaa
     1201 gcggccgaat tgcgtgatgg cgacaagaag cgctacctcg ggaaaggcgt actgaaggcg
     1261 gtggagcacg tgaacgaaac gctcaataac gaactgcacg gcgccttggt gaccgagcag
     1321 gccatgatcg acaaggccat ggtcgggctg gacggcaccc ggaacaaggg caacctgggc
     1381 gccaatgcca tcctcggcgt cagcctcgcc gtggccaagg ccgcagcgag cgaggcgggc
     1441 ctcccgcttt accggtatgt gggcggatcc aacgcacgca ccctgcccgt gccgatgatg
     1501 aacatcctga acggtggggc ccatgccgac aacaaggtgg acatccagga gttcatgatc
     1561 atgcccgtgg gagcggacag cttcgcccag ggtctgcgca tgggggccga gactttccac
     1621 cacctcaagg cggtgctgaa gtccaagaac ctcggcacca atgtgggcga cgagggtggt
     1681 ttcgcgccgg acctcaagag caacgaggat gccctcaagc tcgtgatgca ggccattgag
     1741 aaggccggat acgaaccggg cgaggacatc gtgatcgcgc tcgactgcgc cagcaccgag
     1801 ttctacgatg cgaagaagaa gcgctacgtg ctggagagta ccggtgacag ccttaccagc
     1861 gacgacatgg cggccctgct ctccgggtgg tgcaagaaat accccatcgt cagcatcgag
     1921 gacggcatgg ccgaggacga ttgggacggt tggaagaagc tcaccgaggc gatcggtggg
     1981 aaggtgcaac tcgtgggcga cgacctgttc gtcaccaata cggagcgact ggccaagggt
     2041 atccaaggcg gcatcgccaa ttccatcctc gtgaaggtga accagatcgg tacactgacc
     2101 gagacactgg aagcggtgga cctcgcgcag cgcaacagct acacggcggt gatgagccac
     2161 cgcagcggcg aaacggagga cagcaccatc gccgaccttg ccgtggccgt caattgcggt
     2221 atgatcaaga ccggcagcgc cagccgcagt gaccggatcg ccaagtacaa ccagctgctg
     2281 cgcatcgagg agcagctcgg caagagtgct atttggcctg gccggaggct tcgatatgtg
     2341 ggctgaaccg gcggtagcgg ttcagcgggt gatgttcacc tgacccagca tcacttcccc
     2401 gatcccctgg atccgtgcgg atacgaacac gctctgcgcg gcgataccgg tgatgttcaa
     2461 ccgggtgatg ttcgcggatg cgggccggtc gatcagcgta cgtccgagcg tgtcgcgtac
     2521 gatgatccgt gcgtcgagac ccctgaccgc ccgcggtgct tccatcacca ggtgttcact
     2581 cgctgggttg gggtataatt tccatccgcg tagcgcatcg ccgctggtcg ccacgccggt
     2641 cgcgttctgc cgcaggccga tgcaccaatc ccctggcgtc atgccgtgcg cggtgagccg
     2701 cgcgtagccg tcgctggcat cgctgccgat cgtattgatc tccgtgtccg catggatgct
     2761 ccacggaaaa tgggcgttcg ggcggtacag catcagcagg ctgtcctcct ggaaacctga
     2821 cccaccgatg tccgcgacca gtccggggtc gaagcatgtg ccgctcgtgg gtcgaccgtc
     2881 cagcgtgagg cgcaggtcca tcgccgtgcc ctgcgccggt cgtccggtga tgcgccacca
     2941 ccgatcgggc gaagccacgg atcccgatcc ttctccggtg gcgtccgcag cggtccagaa
     3001 ctcctcaacg cgcatgggtg cggctgacgg gagcacgctc gtgatgagtg atacgtcggc
     3061 cagcagcagg ttcttcgtgc ccgtgttcgt cacggtgtcc tcagtgatcg tggtcgccaa
     3121 cgcgaggagc tgttcggcgt tcaggaaggc ggcgacgggt tggaagggcg gcgtgctgac
     3181 ggcagttgaa tgctcatttc cgagtatcgt ccgttcggga tcggtccagc gcgtgccatc
     3241 ggcatcctcg aaggtgatgg tcaccggtac gttgtggtag aaatggtcgg catggcgcag
     3301 cttctgctgc acgtacagtg ttgtgggcca tgcaccgccg tcctgcgccg tggtgaacga
     3361 gtccacctcg aacgcggccc aacccggttg catgatccag tcggcgaaat agtccgtcag
     3421 gtcggtcccg gtggccacgc tcaagctgtc gcgaagctcc tcggtgccca tgtcgctgta
     3481 cgcattgttg gtcagcacgc gggtgtagcc gctcgcgaac agcgagtcgc ccaggtagcc
     3541 acgcaaggtg tgcgcgaggt cggcaccctt ccggtaggaa tgttcgccgt aggtatgttc
     3601 ctgtggcaca ttgttcagcg cccaccatcc ttcgtcctgc aaatgtgccg tgctgaccat
     3661 gtcgtggtga ttgttgcgca cggtacgcat gtaccggtca cgaccgtaca catcctccag
     3721 gaagagatag ctcaggaatt cagcgtaccc ttcgttgatg tacatctctc cggcatgtcg
     3781 gcacgtgatc aggtcgccga accaatgatg cgccagctca tgcgccatga cgtcctcgta
     3841 ctgcagcgtt ccatcggcga tggatcccgg ataactgatg ttggtaggat gctccatggc
     3901 tccctgggtg gtcaggcagt atcccacgcg ttcccagcgg tacggtccga accaccgctc
     3961 gaacgtgtcg aaggcgtcgt gcaggtgctc gaatgaattc ttgagatccg tggtatcatt
     4021 cggcttggcc gccaaggcga cgggaaccag tgccccgctg atgctcggga acgagtcgcg
     4081 caccgccaca taattcgtgg cggacacgga ggcgaggtag gaggggatcg gctcttcgat
     4141 gcgccaatgg aacaggcgct gaccatcgcc ctcgtccacg tcggcgatga gtgtcccgtt
     4201 ggcgaagacc gtgcggccac cattcacgcg cacctggaac tcgaaggtgc agcgctccac
     4261 gaagttgtcg aagcagggga accaggcacg cccgtatgaa tgtggtacgg cgtcgaaggc
     4321 cacgcccagg ttgtattggt acgccgacgt ggtgtagaat ccgccccagc cactggcgtc
     4381 caccgtgggg ttgccgccgt agtggatcac aacctccagt gtatccgccg gaccgtgcgc
     4441 tacgccgagg tcgaccagca attcttcccc ggtgtgcatg aagttcagcg gatcgccgcc
     4501 cgtttgtcgc accgagtcga ccgtgagatc gagcaggtcc agcggcaagg tggtgatgcc
     4561 ctccacgcga ggaacgaagc ggatgtgggc gctgccgtgg atgagttgcg acccggtttc
     4621 ccgcaggtcc agctcgatcc gggtgtgcag caggtcgagg ctgtcgctcc tggtgaggct
     4681 gcccgtttgt gcggccagtg ggagcgtgga aaggagcgtg atggccgcta cggtcgttcg
     4741 gcccggtgat ggtctggtca tggatcggtg atccggacga atgtatggcc ccgggtaccg
     4801 cttcagcctg cgctggacaa ggccgctcag gatccctatg ttcgggacaa ccagttggga
     4861 tcctgccacg tctatttcat ggccatgaaa ccgagtacgg aacttttcga cctcatccgt
     4921 tcgctcacca agtcggagaa gcgcttcttc aagctgcaca gcaccttgca aagcggcacg
     4981 aagaattaca tgcggatctt cgatgccatc gaccggcagc acgtttacga cgaggacgcg
     5041 ttgcgtcgtc aattcgaggg tgagaatttc gtgaagcatc tgccctcgga gaagaaccat
     5101 ttgtacaagc tggtcctgaa gtcgcttcgg gccttccatg cggaagccac ggtcagcgga
     5161 acgttgaaga tcgcgagcaa gaacatcgag atcctgttcg ccaaggccct gtacgaggag
     5221 gcatcgcgca cgctgcagcg cgcgaaacgc gtcgcgaagg agaatgaaaa attctactac
     5281 tggtacgaat tgttgaacca ggagaagcag cttctggaag aggactatgc gagcggacgc
     5341 ttctcacgcg acctggagga cctcgtggcc gaggagcggg aggtgctggc caagctcggc
     5401 aacctggcgg agtaccatgt tctctacagc cggatcaatt tcgtgttccg gagcgggggc
     5461 cacgcgcgca cggacgagga gcgggccatg gtggaggcca tcagtgagca cccgctgatc
     5521 aaggggaaga acaccgccct gagccaccgt gccgccacca tctgctatta cacgcaaggc
     5581 ttctgccagt gggccgccgg cgatacggaa cggagcctgg agaaattcct ccgggtgaaa
     5641 aaaatacttg atgatcatcc tcatctgcgt gccgacctgc cgaaacgcta cgtgcgcgcg
     5701 ctggagtaca tcatacggtg ccgggtggag ctcggtgcgt tcaaggaggc ccgcgatacc
     5761 atccggctga tgcgccagtc ggccatccag gacggtttcc ggggcatcga tatcgcggtg
     5821 caggtcttcc acgcgagtta cctcggcgaa ctgcggatgc tggaccggat gggcgagcac
     5881 atgaaagcgg tggccctgac cacggacatc ctgcatggta tcgaggagca tggtgcgcgc
     5941 ctgcacaagg agcagttgct ggagttctgg tcaggactgg ccatcgccca tttcggcgcc
     6001 ggccaggtga acaaggcgct gttctggttg aacaaggtac tgaacgaccc cgaaccccag
     6061 ttgcg
//
LOCUS       DAOQOC010000025         6007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5180_length_6007_cov_3.005208, whole genome shotgun sequence.
ACCESSION   DAOQOC010000025 DAOQOC010000000
VERSION     DAOQOC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_6007_cov_3.005208"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1949)
                     /locus_tag="PL106_00735"
     CDS             complement(225..1949)
                     /locus_tag="PL106_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31618.1"
                     /translation="MTRLLTLTFLAASAAMSASAQTPVPAPIFGTNGCAEIAFPAGTL
                     AGLKSHALSPDGKLLLAGWGGSGFPYYACMARIDTACGELDLTFGLNGAQAHHFEQRT
                     ICSTVALQADGKIIGGGLIAASNAGSGQFPGVWRYHSDGSVDSTFNGTGYNNIGFPTG
                     SATGKVEEVFIDADGHILAAIIGNAQLGVFRYTTDGVLDTSYSADGRAVMPVGYTPFN
                     EDLGAVMDPDGSVTIAGLVGTSAFDPYFMALARFLPNGDPDPAFGTNGLAMHPNVSTS
                     DLGTPDGFKNWSMVRRPGGGYLVGYGAHDGDTRPRVAAFTDAGALDLSYGTDGIYQFT
                     GSNPVGSGLWMEADGSVLLFIRLSNVSNGPGAIVKLMPNGQPDASFGTNGLVQSPFNT
                     RSIAHGLRLSGGDVIAYGINNNNGDGSVVRFSLDANANALPVITYAFPDLVVSGGGSI
                     QWFLDGLPISGATGSTHTPTQNGTYTVEMTSFGCINTTPPFQFLSTGTADVVPSELVI
                     QQDLAAGTLHIRNDGAPTPWSMMDASGRELRTGLFATGSSEIDLAHLRSGLYLLRSKD
                     CVHRFVMP"
     gene            2083..3066
                     /locus_tag="PL106_00740"
     CDS             2083..3066
                     /locus_tag="PL106_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNA31619.1"
                     /translation="MQKLSTALFSACIALMPVRASAQLPPYVPQDNLVGWWPFTGNAD
                     DISGNAHHGTLNGAVLAADRFGMNDRAYAFNGTSDHIEVADAPALRLGDASYTIAWWA
                     QVDTYNANATAFVVKRGAGSYNGWMVVANGSYENKIEMKTSSGADPEIRCDAAMVTGS
                     WFHFAIVTDVAVDSIYFFANGSEVYRQASGGLYYNPNSTTPMRFGSDTSIPVGTYFLS
                     GSMDDIGIWSRRLTAQEVLDLYLNTNVSVSEIAPEVASVVFDGTTGELILRVPQAFVG
                     TAYSVLDASGRLVQAGTLRSDVTRLAARDRNGALLVRFDPVPGDAVRVVIP"
     gene            complement(3013..3492)
                     /locus_tag="PL106_00745"
     CDS             complement(3013..3492)
                     /locus_tag="PL106_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA31620.1"
                     /translation="MATTKKPLDWKLVPAPESSDHVRIDKQYELFINGKLQKPRWGKY
                     FNPITPANEQKLARIAQAGEADVDAAVKAARKAYDTVWSRMPAPERGKYIYRIARLLQ
                     KNARGFAIIESTGADKDDLEWCHMDLPFATATLPARCALPSWDDNTHCIPRHGIETH"
     gene            3536..3802
                     /locus_tag="PL106_00750"
     CDS             3536..3802
                     /locus_tag="PL106_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31621.1"
                     /translation="MARLIIDIQDPKKENEVADLLLKIGGVEIERAEASPNKSTKRKA
                     GIKRTLTAKEKKFVSELRQALKEVNDHVTGRKPFQPAREFLHGL"
     gene            3792..3947
                     /locus_tag="PL106_00755"
     CDS             3792..3947
                     /locus_tag="PL106_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31622.1"
                     /translation="MGYRFGVSPTFRKKAKRLAKKYASLKEELLDLVTRLERDPEQGT
                     PIGKRCF"
     gene            complement(4254..5393)
                     /locus_tag="PL106_00760"
     CDS             complement(4254..5393)
                     /locus_tag="PL106_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31623.1"
                     /translation="MAKKKTFDWSLAPTPESADHVKINAQYELFINGKWQKPRSGTRS
                     TGSGEPYFDTINPANEKVLARIAEAGDAIARTRGTVADIASMIKALLQPLRSALFFLS
                     VLATMAAQAQGGGCSITLLSADSTDEQAFCADPVNVTIDPVVYMIEGTGLQVTGLPPG
                     VVSTFANDTLTLTGTITTTGVWYPDITLNEGCSADLYFSVSQSVDPGFSCTVVGDSIV
                     VSWPGLDAPTQQDGTMFLFWTDGVDLLGQTVYFLPCPGSLTVGGLPANTPITFSMSLV
                     SGMPNCLEQGDATTCTIISTGIGDPGADEFLVRAVPRGDVLELSSSVALHDLRIYDML
                     GGLVLSHRMNARIANVPIGCVAPGAYVLRVVDADGRVSAQRFVKE"
     gene            complement(5455..>6007)
                     /locus_tag="PL106_00765"
     CDS             complement(5455..>6007)
                     /locus_tag="PL106_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNA31624.1"
                     /translation="NMSEWRNFLVALKSNRYDAAVLLVTFLLTVVFDLVIAIEVGMVL
                     AAFLFMKRMSDITDMKPVLGAANGEEHLFDEEVGALPGHVLLFEISGPLFFGAAQKFR
                     DVLGEINDRHRVVVLRMRQVPLVDATGLHRLGGIVKHLQAHRRKVFLVDIDPRVREEL
                     IGQDWLRDGMIQPSLAEAIASAE"
BASE COUNT         1201 a         1769 c         1909 g         1128 t
ORIGIN      
        1 gcttctgcca cttgccgttg atgaagagct cgtattgcgc gttgatcttg acgtggtcgg
       61 tggactcggg ggcgggggcg agggtccagt cgaggggctt cttcttggtg gccatgcatt
      121 gatcgtcgta gctgagttgg cgaagacgag ggtcgaagat agcggcccga gaacaagcgt
      181 gcccgtccag aaccgaacgg gcacgctacg atgctggggt atgatcaagg catcacgaag
      241 cgatgcacgc aatccttgga acgcagcagg tagaggccgc tgcgcaggtg cgcgagatcg
      301 atctcgctgg aaccggtggc gaacaagccc gtgcgcaatt cgcgtccgct cgcatccatc
      361 atgctccacg gcgtgggcgc accatcattg cgtatgtgca gcgtgccagc cgcgaggtcc
      421 tgctgtatca ccagctccga tggaaccaca tcggcggtac ccgtgctgag gaattggaac
      481 ggcggcgtgg tattgatgca gccgaaggag gtcatttcca cggtgtaggt gccgttctgc
      541 gtgggcgtgt gcgtgcttcc ggtagctccg ctgatgggca ggccatcgag gaaccattgg
      601 atgctcccgc cgccgctcac caccagatcg gggaaggcat aggtgatcac gggtagcgcg
      661 ttcgcgttcg catccaggct gaagcgcacc acagaaccgt caccgttgtt gttgttgatg
      721 ccgtaggcga tcacgtcgcc accactgagg cgcaagccgt gcgcgatgct gcgtgtgttg
      781 aacggtgact ggaccagccc attggtaccg aagctcgcgt ccggttgtcc gttgggcatc
      841 agtttgacga tggcgccagg gccattgctc acattgctga gccggatgaa gagcaagacg
      901 cttccgtcgg cttccatcca cagcccgctg cccaccggat tactcccggt gaattggtag
      961 atgccgtccg tgccgtagct caggtccagc gctcccgcat ccgtgaatgc cgcgacacgc
     1021 ggccgtgtat cgccatcgtg cgcgccatag cccaccaggt atccgccacc cgggcggcgc
     1081 accatactcc agttcttgaa cccgtccggg gtacccagat cgctggtgct cacgttggga
     1141 tgcattgcga ggccgttggt gccgaaagcc ggatcggggt cgccattggg caggaagcgc
     1201 gcaagtgcca tgaagtacgg atcgaaggca ctcgttccca ccaggccggc gatcgtcact
     1261 gaaccatcgg ggtccatcac cgcgccaagg tcctcgttga acggggtata gcccaccggc
     1321 attaccgcgc ggccatccgc ggaatagctg gtatccagca cgccgtcggt cgtatagcgg
     1381 aacacgccca gctgcgcgtt gccgatgatc gcggcgagga tgtggccgtc cgcatcaatg
     1441 aagacctcct ccaccttgcc cgtggcggaa cccgtcggga agccgatgtt gttgtagccg
     1501 gtcccgttga aggtgctgtc caccgatccg tcagaatggt agcgccacac accggggaac
     1561 tgccccgatc ctgcattgct tgcagcgatc aagccgccac ctatgatctt cccatcggcc
     1621 tgcagcgcaa cggtgctgca gatggtgcgc tgctcgaaat gatgggcctg cgcgccgttc
     1681 agtccaaaag tgaggtcgag ctcaccgcaa gcggtgtcga tccgcgccat gcaggcgtag
     1741 taggggaacc cgctaccgcc ccagccggcc agcaacaatt tgccatcggg cgatagtgca
     1801 tggctcttca ggcccgccaa ggtccctgct gggaatgcga tctcggcgca gccgttggtg
     1861 ccgaagatcg gagccggcac aggggtttgt gcactcgcgc tcatcgctgc ggatgcggcc
     1921 aggaaggtga gggtcaggag tcgggtcatg aggcgtgttg tttcggaccg ccaagcaagt
     1981 gggatataca gcgtggcaca atagaacttt cgttagtggg caggtgcaat tccgccgcca
     2041 cacagcgtgc gaggagtata ttcgttcgcc aacccgagtg ccatgcagaa gctcagcaca
     2101 gccctcttca gcgcatgcat cgcgctaatg cctgttcgtg caagcgccca actgccgccc
     2161 tacgtgccgc aggacaacct cgtgggctgg tggcccttca ccggcaacgc cgacgacatc
     2221 agcggcaacg cccaccacgg aacgcttaac ggtgccgtgc tggctgcgga ccgcttcgga
     2281 atgaacgaca gggcgtatgc gttcaatggc acgagcgacc acatcgaggt cgccgatgcg
     2341 cccgcgctca ggctgggcga tgcctcttac accatcgctt ggtgggcaca ggtggataca
     2401 tacaatgcga acgcgacggc cttcgtggtg aagcgtggtg ctggctcgta taacggatgg
     2461 atggtagtgg ccaatgggtc ttacgagaac aagatcgaga tgaagaccag cagcggggcc
     2521 gatccggaga tccgctgcga tgcagcgatg gtaaccggct cctggttcca cttcgcgatc
     2581 gtgaccgacg ttgcggtgga ttccatctat ttcttcgcga acggaagtga ggtgtaccgg
     2641 caggccagtg gcggcttgta ctacaacccg aacagcacga cgcccatgcg tttcggcagc
     2701 gataccagca ttcccgtagg cacctatttc ctgtccggct ccatggacga tatcggcatt
     2761 tggagccgtc gcctcaccgc gcaagaggtg ctggacctgt acctgaacac gaacgtgagc
     2821 gtgtcggaga tcgcaccaga ggtcgcgagc gtggtgttcg atgggacgac gggcgaactc
     2881 atcctgcgcg tgccccaggc attcgtcggt accgcgtact cggtgctcga tgcaagcgga
     2941 cgcttggtac aggcgggcac cttgaggtcc gatgtgacgc ggctggcggc gcgcgatagg
     3001 aatggcgcgt tgctagtgcg tttcgatccc gtgccggggg atgcagtgcg tgttgtcatc
     3061 ccatgatgga agagcgcaac gcgcgggaag ggttgctgtg gcgaatggca ggtccatgtg
     3121 gcaccattcg aggtcgtcct tgtcggcgcc ggtgctttcg atgatagcga acccgcgggc
     3181 gttcttctga agcaggcgcg cgatgcggta gatgtacttg ccacgctcgg gggcgggcat
     3241 cctgctccac accgtgtcgt aggccttgcg tgcggctttc acggcggcgt ccacatcagc
     3301 ctcgcccgcc tgggcgatac gggcaagctt ctgctcattg gcgggggtga tggggttgaa
     3361 gtacttcccc catctcggct tctgcaattt accgttgatg aagagctcgt attgcttgtc
     3421 gatgcggacg tggtcggagg actcgggggc gggaaccagt ttccagtcga ggggcttctt
     3481 cgtggttgcc attgtgagcg aaggtaagca gtgtatacgc tatcatcgca aagacatggc
     3541 gcgactgatc atcgatatcc aggaccccaa aaaggaaaac gaggtcgccg acctgttgct
     3601 gaagatcggc ggggtggaga tcgagcgcgc cgaggccagc cccaacaagt ccacgaaacg
     3661 caaagccggg atcaagcgaa cgcttaccgc gaaagagaag aagttcgtgt cggaactgcg
     3721 ccaggcactg aaggaggtca atgaccacgt tacaggcagg aagccgttcc aacccgcacg
     3781 cgagttcctg catgggttat aggttcggtg tatcacccac cttcaggaag aaggcgaagc
     3841 gattggcgaa gaagtacgct tccctgaagg aggaactact ggacctggtg acacggcttg
     3901 agcgggatcc cgaacaaggc acgcccattg gcaagcgttg cttctagatc cgtctatcga
     3961 tcaagtcaaa aggaggtggg aagtctggag gtgcaagagt gatcacgtgc gtggtccacg
     4021 ttcagaagga agttcgcttg ctgacgatct acgacaagtc cgaacaggga acgatcacgt
     4081 cgcgagagct tcagtccctg cttgccgaaa tgtcacgtga acgataggtt cagaaacccc
     4141 actccacctg acctctcgat gcggacgtgg tccttgctct caagggctgg agcgagtttc
     4201 caggagatca tcttcgtctt ggccatgcaa cgaagatagc gggccccgcg cgctcactcc
     4261 ttcacgaagc gctgcgcaga cacgcgacca tcagcatcga ccacacgaag cacataggct
     4321 cccggtgcca cgcagccgat gggaacgttg gctatccgtg cgttcatcct gtgcgacaag
     4381 acaaggccac cgagcatgtc gtagatgcgc aggtcgtgga gcgcgaccga agacgacagc
     4441 tcgagcacat cgccacgagg cacggcgcgc acaagaaatt catccgcccc gggatcaccg
     4501 atgcctgtgc tgatgatcgt gcaggtggtt gcatcgcctt gttccaagca attgggcata
     4561 cccgatacaa gggacatgga gaacgtgatg ggcgtattgg ctggaagacc accaacagtg
     4621 agggaaccag gacaaggcag gaagtacacg gtttggccga gcaggtccac gccatcggtc
     4681 cagaacagga acatggtgcc gtcctgctgc gtcggcgcat ccaggcctgg ccaactcacc
     4741 acgatgctgt cccccaccac cgtgcaggaa aatcctggat caacgctctg cgaaacactg
     4801 aagtacaggt ccgccgagca tccttcgttc aacgtgatat ccggatacca aacacctgtg
     4861 gtggtgatcg taccagtaag ggtgagcgtg tcgttcgcga acgtgctgac cacaccgggc
     4921 ggaagacctg tcacttgtaa accggttccc tcgatcatgt acacgaccgg atcgatggtg
     4981 acattgaccg gatcggcaca aaacgcctgt tcgtcggtgg aatccgcgga aagcagcgtg
     5041 atggagcagc caccgccctg ggcctgtgcc gccatcgtgg ccagaacgga taagaagaac
     5101 aacgcggacc gcaagggctg gaggagtgct ttgatcatgg atgcaatatc cgcaacagta
     5161 ccacgagttc tcgcgatggc gtcgccggcc tcggcgatac gcgcgagcac cttctcgttg
     5221 gcggggttga tggtatcgaa gtacggctcc cctgagcctg tcgaacgggt gccgctcctg
     5281 ggcttctgcc acttgccgtt gatgaagagc tcgtattgcg cgttgatctt gacgtgatca
     5341 gcggactcgg gggtgggggc gaggctccaa tcgaatgttt tcttcttggc catgcattgt
     5401 acgccgaagc cagaagggcg aaggcgaggg tctaagattg agggcgttaa cagctcattc
     5461 ggctgatgcg atcgcctcgg ccagcgaggg ctggatcatc ccgtcccgca gccagtcctg
     5521 cccgatcagt tcctcgcgca cacggggatc gatatcgacg aggaagacct tgcgccggtg
     5581 cgcctgcaag tgcttcacga tgcccccgag ccggtgcagg cccgtggcgt cgaccagcgg
     5641 tacctggcgc atgcgcagca ccaccacccg gtgccggtcg ttgatctcgc ccagcacgtc
     5701 ccggaacttc tgcgccgcgc cgaagaagag cggcccgctg atctcgaaga gcagcacatg
     5761 cccgggcagc gcacccactt cctcgtcgaa caggtgctcc tcgccgttcg ccgcgcccag
     5821 cacgggcttc atgtcggtga tgtcgctcat gcgcttcatg aacaggaagg cggccagcac
     5881 catgcccacc tcgatggcga tcaccaggtc gaagaccacc gtgagcagga aggtgaccag
     5941 cagcaccgct gcgtcgtagc ggttgctctt cagggccacg aggaagttgc gccactcgct
     6001 catgttg
//
LOCUS       DAOQOC010000026         5982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5210_length_5982_cov_3.446432, whole genome shotgun sequence.
ACCESSION   DAOQOC010000026 DAOQOC010000000
VERSION     DAOQOC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5210_length_5982_cov_3.446432"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..836
                     /locus_tag="PL106_00770"
     CDS             45..836
                     /locus_tag="PL106_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNA31625.1"
                     /translation="MTEPIRIPTMAEMMASGDTPEVLFWVGCAGSFDDRAKKITKAFV
                     RILNAAKVKFAVLGTEESCTGDPARRAGNEFVFQMQAQNNITVLNGYGVKRIVTACPH
                     CFNTLKNEYPALGGSYEVMHHTQFINALMKEGRIKVEGGSFKGKRITFHDPCYLGRGN
                     GEYEAPREVLLKLDAALVELKRSKQNGLCCGAGGAQMFKEAEPGKKEVNIERSEEALA
                     ASPNVIAAGCPFCNTMLTDGVKHFNKEGEVVVKDVAELIAEAGEL"
     gene            complement(841..2448)
                     /locus_tag="PL106_00775"
     CDS             complement(841..2448)
                     /locus_tag="PL106_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31626.1"
                     /translation="MRRPYTGAFTALSAFGLALDMSAQGPALAWESFFDGSLPGVDQA
                     NALLVAGDNSVYVTGQSTNLAPQGTITTLHYGSSGQQIFVDHVYGAAQETVNKGVALA
                     SDNIGHVYVCGAISNNGGDLALIKYRSSGRVWRENYEQYPNAEVEDQATGVAVDAEGR
                     VYLAGYITSTSGMGYDHFLIATDSAGSVLWADDPALSSADEFAFDVAISASGQPLLAG
                     DWWNVDGSSGIDMSTLRYTVNGTHLWDRSFEGVGSDDHLRSLLATADDGAVLCGHAMG
                     DSDQDMTVVKFDAVGSQEWAVQYPGSGNADDEAVEAVELSDGRIAVVGRSRELVDGSL
                     RHAIIVLVIDAGVVVWNAQYTGENELGAWPTGIATDGLDNIHVSGYAVGAGGDYTDGV
                     VLSYDADGTLAWSIGYDGPAGFDDRFNDIALNSMGDVLVCGSTGTSATATRYVTVQYG
                     NAVGVDEAGRRPEALDVRQGDRANCFVINGLSPGEGLRALDALGNELPLTGHRSGMRY
                     DAQLPVSAGCYLLQATETWRIARVVVR"
     gene            complement(2445..4115)
                     /locus_tag="PL106_00780"
     CDS             complement(2445..4115)
                     /locus_tag="PL106_00780"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNA31627.1"
                     /translation="MAHTKIPLLTRPKDDGKRTFSVALIALVGLSTVHAQLDLDGSIS
                     PAQLVQHTLLGTGVTVSNVTYNGLPGNTPLESIGRFDGSDCVLGIDSGIVMCTGGINV
                     AQGPNNSGSMQMELSDVTPSDDDLENVAGDLCMDVATLEFDFVPQGDSITFAYSFASE
                     EYLEYVNAGYNDAFGFFLSGPGIFGPFQNGAVNLAVVPGNFSYVSVNSVNTGSNSQYY
                     QDNGSGSSAPYNTNPYYIQFDGFTVGLRAGAVVQCGQTYHIKMAIGDVGDPNWDSGVF
                     IQGGTFSSTGGLSVSISTPAMDGTIAEGCGQAIVTLTRSTSTGELVIPLSTVGSATIG
                     VDFSPLPASITIPEGQASASFVIDIPNDDLTELDETLIIQALFTGACGDAPVSAQLII
                     HDHPPLVVTGQEVNTTCSDALTTIAPVVSGGIGPLGYVWNDGSADSTLNVPGHSGVYY
                     VTITDACGLVAEAGINVFAPCEVVVPNVFTPNSDGENDRFVVEGLEGTRNTVEVFNRW
                     GQLVYQASNYANNWEAHGLSDGTYYYVIKVEGYSSAFTGHVTILANRS"
     gene            complement(4323..>5982)
                     /locus_tag="PL106_00785"
     CDS             complement(4323..>5982)
                     /locus_tag="PL106_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA31628.1"
                     /translation="NHGSWGGESDAYAIFLTQEAYDRFKMTKEEAALAKEDEEKKEGG
                     DEKKEDKDKKNKDDKKKEPEKDKQVEPIRIELAGIEDRRVRLTPNSSALTGAVLSNDG
                     EKLYYLAAFEKGTDLWQYEIRTREAKILNKMGDGWAGGLEWDKDQKALFYISNGTVSK
                     YDTEKNENKSIGINGEMLLNENAERAYLFEHIWRQVKKKFYRTDMQGVDWDKYKAEYQ
                     RYLPYINNNFDMAELCSEMLGELNASHTGSGYRPEVKNGDETATLGCFFANGPGPGLR
                     ITEVMEKSPLIKQGTKVKAGHVIEKIDGVTITADIDWAMLFNRKGGKPMLLGMFDPGT
                     NTRWEETVKPLAGGGDQELLYHRWVERCRHLVDSLSGGKLGYVHVEGMDDNSYRTVYE
                     EALGRYHDKEGLVVDTRFNGGGWLHDDLATFLNGKTYMTMLPRGQNLGTEPQFKYQKK
                     SCVVMSESNYSDAHMFPVTYRALGVGKLVGMPVPGTGTAVWWETLQNGMWFGIPMVGM
                     VDNNGNYLENQQLEPDVKQPLDPGVVSQGRDQQLEAAVKLLLGQ"
BASE COUNT         1103 a         1851 c         1787 g         1241 t
ORIGIN      
        1 gaataggggg cgacattcat gccgcccgat cactcccaag tatgatgacc gaacccatcc
       61 gcatccccac catggcagag atgatggcca gcggtgacac gcctgaagtg ttgttctggg
      121 tgggctgcgc gggatccttc gacgaccggg cgaagaagat caccaaagct ttcgtgcgga
      181 tcctgaacgc ggcgaaggtc aagttcgcgg tgctgggcac ggaggagagc tgcacgggcg
      241 accccgcacg acgggcgggc aacgagttcg tcttccagat gcaggcgcag aacaacatca
      301 ccgtgctgaa cggctatggt gtgaagcgca tcgtcaccgc ctgtccgcac tgcttcaaca
      361 cgctgaagaa cgaatatccc gcactgggcg ggagctacga ggtgatgcac cacacgcagt
      421 tcatcaatgc gttgatgaag gagggccgca tcaaggtgga ggggggcagc ttcaaaggca
      481 agcgcatcac cttccacgat ccgtgctacc tgggccgtgg caacggcgag tacgaagccc
      541 cgcgtgaagt gctgctgaag ctcgacgccg cgctggtgga gctgaagcgt agcaagcaga
      601 acggtctgtg ctgtggtgcg ggtggcgcgc agatgttcaa ggaagccgag cccgggaaga
      661 aggaagtgaa catcgaacgc agcgaagaag cgctggccgc ctcaccgaac gtgatagcag
      721 cgggctgtcc gttctgtaac accatgctca cggacggcgt gaagcacttc aacaaggaag
      781 gggaggtggt tgtgaaggac gtggcggagc tgatcgcgga ggcgggggag ttgtgacggc
      841 tcagcggacc accacccggg cgatgcgcca ggtttccgtt gcctgcagga gatagcatcc
      901 tgcggacacg ggaagctgag catcgtagcg cattccggat cgatgacctg ttagcggcag
      961 ctcgttcccg agcgcgtcca aggcgcgcag gccttcgccg ggtgaaagcc cgttgatcac
     1021 gaagcaattc gcacggtcac cttgcctcac gtcgagtgct tcgggcctcc ggccggcctc
     1081 atcgaccccc accgcattgc cgtattgaac ggtaacgtat cgggttgccg tggcggaggt
     1141 gccagtggat ccgcacacca gcacgtcccc catgctgttc agcgcgatgt cgttgaagcg
     1201 gtcatcgaag ccggccgggc cgtcataacc gatcgaccag gcgagggttc cgtcggcgtc
     1261 gtagctcagc accacaccgt ccgtgtagtc gccgccggcc cccaccgcgt aaccggagac
     1321 atggatgttg tccagcccat ccgtggcgat cccggtgggc catgcaccga gttcattttc
     1381 acccgtgtac tgcgcgttcc acaccacaac gccggcatcg atgaccagca cgatgatggc
     1441 atggcgcaag gagccatcca ccagctcccg gctccggccc acgacggcga tgcgcccgtc
     1501 actcaattcg acggcttcga cggcctcatc atccgcgttc ccgctgcccg ggtattggac
     1561 cgcccactcc tgtgaaccca ccgcatcgaa cttcacgacg gtcatgtcct ggtcactgtc
     1621 ccccatggcg tgtccgcaca gcactgcgcc atcgtcagca gtggccagca gggatcgcag
     1681 gtgatcatcc gatccgacgc cttcgaagct gcggtcccat aagtgtgtcc cgttaaccgt
     1741 gtaccgcagt gtgctcatgt cgatgccact gctaccgtcg acgttccacc agtcgcccgc
     1801 gagcagaggc tggcctgagg cggagatcgc cacatcgaac gcgaactcat cagcgctact
     1861 gagagctggg tcatcggccc agagcaccga tcctgcactg tccgtcgcaa taaggaagtg
     1921 gtcatatccc atcccgctcg tggacgtgat gtagcctgcc aggtaaacgc ggccttccgc
     1981 atcgacggct acccctgtgg cctgatcctc cacttccgcg ttggggtatt gctcgtagtt
     2041 ctcgcgccaa acccgtccgg aggatcgata cttgatcagt gcgagatccc caccgttgtt
     2101 ggagatagcc ccgcacacgt aaacatgtcc gatgttgtcc gaagcgagtg cgacgccctt
     2161 gttcacggtc tcctgggcag cgccgtacac atgatcgacg aagatctgct ggcccgagga
     2221 accatagtgc aaggtggtga tggtgccctg cggcgcgagg ttggtgctct ggccggtcac
     2281 atagaccgaa ttgtcacctg caacgagcaa cgcgttggcc tgatcgaccc ctgggaggct
     2341 gccgtcgaag aaactctccc aggccagggc cggcccctgt gctgacatgt cgagagcgag
     2401 cccgaacgcg ctgagcgctg tgaacgcacc ggtgtatggt cgtctcatga tcggttagcg
     2461 agaatggtga catgcccggt aaaggccgag ctgtagcctt cgaccttgat cacatagtag
     2521 taggtgccat cgctcagtcc gtgtgcctcc cagttgttgg cgtagttgct ggcttggtac
     2581 accagctgcc cccagcggtt gaagacctcc accgtgttgc gcgtcccctc aaggccctcg
     2641 accacgaagc ggtcgttctc gccgtcgcta ttcggtgtga agacattggg tacgaccact
     2701 tcacagggtg cgaaaacgtt gatcccggcc tcggcgacaa ggccgcaggc gtcggtgatg
     2761 gtcacgtagt agacgcccga atgccccggc acgttcagcg tgctgtcagc actgccatcg
     2821 ttccagacat agcccagcgg tccgatccca ccgctaacca cgggagcgat ggtggtcaac
     2881 gcatcgctgc acgtggtgtt cacctcttga ccggtaacca caaggggcgg atggtcatgg
     2941 atgatgagct gtgcggagac cggtgcatcg ccgcaagcac cggtgaacag cgcctggatg
     3001 atcaaggtct catccaactc ggtcaggtca tcattcggaa tgtcgatcac gaacgatgcg
     3061 cttgcctggc cctccgggat ggtgatcgat gcgggcaggg gtgagaagtc gacgccgatt
     3121 gtcgctgatc caactgtgct cagggggatg acgagctcgc cggtgctggt cgagcgggtt
     3181 aatgtcacaa ttgcttgacc acacccttcc gcaatggtcc catccatcgc tggcgttgag
     3241 atgctcacac tcagaccgcc tgtggaggag aaggtgccac cctggatgaa gacaccgctg
     3301 tcccagttgg gatcccccac gtcgccgatc gccattttga tgtggtaggt ctgtccacat
     3361 tgaacgaccg ctccggcacg aagccccaca gtgaatccgt cgaactggat gtagtagggg
     3421 ttggtattgt agggggcact cgatcccgag ccgttgtcct ggtagtactg gctgttgctt
     3481 cctgtgttta cgctgttcac gctcacgtag gagaaattcc cggggaccac ggcgaggttc
     3541 acggcgccgt tctggaaggg accgaagatg ccgggaccgc tgaggaagaa tccgaacgca
     3601 tcgttgtaac cggcgttcac gtactccagg tactcttcag aagcgaagga gtatgcgaag
     3661 gtgatcgagt ctccctgcgg cacgaagtcg aattccagcg tcgccacgtc catgcacaga
     3721 tcgcctgcga cgttctccag gtcatcgtcg ctcggcgtga cgtcggacaa ttccatttgc
     3781 atggaaccac tgttgttggg cccttgggcc acgttgatgc cgccagtgca catcacgatg
     3841 ccactgtcga tgcctagaac gcaatccgat ccatcgaacc tgcctatgct ctccaacggg
     3901 gtattgccgg gcaggccgtt gtaggtgaca ttgctcacgg tgacacccgt acccagcagc
     3961 gtgtgctgca cgagctgggc gggggatatc gatccatcga ggtccaattg agcatggacg
     4021 gtcgaaagac cgacgagggc gatcagcgcg accgagaacg ttctctttcc atcgtccttc
     4081 ggccgggtga gtaacgggat cttggtgtgt gccatgctcg ttgcccttgt tcgcgacgca
     4141 aagaggagga agagccaggc aagctcccag cgcaaatgga cgaggacgga aaatgggcgg
     4201 acctgaccgg catcagtcct gccgaaacat cggtcacgtg cgtcgaacgc gcgcacgcga
     4261 agaatgggga aggcccggtc ttccgggcct tccgatagca catgacgacg ggcgcgggtc
     4321 gtctactgac cgagcaagag cttcactgcc gcctccaatt gctggtcgcg gccttgagac
     4381 acgactcccg gatcaagggg ctgcttcaca tccggctcca gttgctggtt ttccaggtag
     4441 ttgccgttgt tgtccaccat gcccaccatg gggatgccga accacatgcc gttctgcagg
     4501 gtctcccacc acacggcggt gcctgtgccg ggcacgggca tgcccacgag cttgcccacg
     4561 cccagtgcgc ggtaggtcac ggggaacatg tgggcatcgc tgtaattgct ctcgctcatc
     4621 acgacgcaac tctttttctg gtatttgaac tgcggctcgg tgcccaggtt ctggccgcgg
     4681 ggcaacatgg tcatgtacgt cttgccgttg aggaaggtcg ccagatcgtc gtgcaaccat
     4741 ccaccgccgt tgaagcgagt gtccaccacc agtccctctt tgtcatggta gcggccaagc
     4801 gcttcctcat agacggtgcg gtagctgttg tcatccatgc cctccacgtg gacgtagccg
     4861 agcttgccac cgctcaggct gtccaccaga tggcggcaac gttccaccca ccggtgatag
     4921 agcagttcct gatcgccccc tccggccagt ggcttaacgg tttcctccca gcgcgtgttc
     4981 gtcccggggt cgaacatgcc caacagcatc ggcttgcctc ctttgcggtt gaagagcatt
     5041 gcccagtcga tgtcggcggt gatggtcacg ccatcgatct tctcgatcac gtggcccgcc
     5101 ttcaccttcg tgccctgctt gatcagcggg ctcttctcca tcacctcggt gatacggagg
     5161 ccaggccccg gtccgttggc gaagaagcaa ccgagcgtgg ccgtttcgtc gccgttcttg
     5221 acctcaggcc tgtagcccga accggtgtgt gatgcgttga gttcgccgag catctcgctg
     5281 cagagttcgg ccatgtcgaa gttgttgttg atgtacggca ggtagcgctg gtactcggcc
     5341 ttgtacttgt cccaatcgac cccctgcatg tccgtacggt agaatttctt cttcacctgc
     5401 cgccagatgt gctcgaagag atatgcgcgt tcggcgttct cgttcaacag catctcaccg
     5461 ttgatgccga tgctcttgtt ctcgttcttt tccgtgtcgt acttgctcac cgtgccgttg
     5521 ctgatgtaga aaagcgcctt ctggtccttg tcccattcaa gcccaccggc ccaaccgtcg
     5581 cccatcttgt tgaggatctt ggcctcgcgc gtgcggatct cgtactgcca gaggtcggtg
     5641 cctttctcga aggcggccag gtaatacaac ttctcaccgt cgttgctcag taccgcaccg
     5701 gtgagggcac tgctgttggg tgtcagtcgc acgcgccggt cctcgatccc ggccagttcg
     5761 atcctgatcg gttccacctg cttgtccttt tccggctcct tcttcttgtc gtccttgttc
     5821 ttcttgtcct tgtcctcctt cttttcgtcg ccaccctcct tcttctcttc atcctccttg
     5881 gccagtgcgg cctcctcctt ggtcattttg aacctgtcgt acgcctcctg cgtgaggaag
     5941 atggcgtaag catcgctctc gccaccccag ctcccgtggt tc
//
LOCUS       DAOQOC010000027         5949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5257_length_5949_cov_3.642178, whole genome shotgun sequence.
ACCESSION   DAOQOC010000027 DAOQOC010000000
VERSION     DAOQOC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_5949_cov_3.642178"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PL106_00790"
     CDS             complement(<1..376)
                     /locus_tag="PL106_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNA31629.1"
                     /translation="MRTGRHIVIVLAAWLSATTSRAQQDPMYSQYMFNTLAFNPAYAG
                     SADVLSAMILSRHQWVGFAGAPSTQTLTVHSPLPGRTLSLGGTLIHDVAGPAKQNCAF
                     LDVAYRIRAGQDARLSFGLKGGI"
     gene            complement(373..5859)
                     /locus_tag="PL106_00795"
     CDS             complement(373..5859)
                     /locus_tag="PL106_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA31630.1"
                     /translation="MMTVTQNAAPDPGEDADITVCEGTSQVDLFPVLGGSPDAGGTWQ
                     AIGSIAPGTLNSGVFFCATNPPGTYHFRYNLPGNGNCPPADAEVIVHITGMLNAGTNG
                     TCTVCDKNPAYNLFNCLGSGPQPGGIWKALPGGQVVGQNLDATVLTAGTTYQFRYVLT
                     GSLSCASDSAVASVTVAHGLFAGNDVTVPLCGNASSTPLFPYLTGAEAPGTWRKTVPP
                     GPFSGSYNPATDNPQVFLYITQNSSPCQNDTARVTVQEVAPPNAGCSPGSITRCQNGP
                     AFDLTQILTCSPAPNGTWRDPSNTVHNSTFVPGVDPAGTWTYTVNGASPCGPAMIALT
                     IVVNPAANAGLDGDTTVCENSSSFQLANVLNGAYAPGGTWKDEGGAITGGLYAPGTLP
                     FDPDGHTYTYVITGASPCPNDTARVTVFENPLPDAGVSNIVQHCLTGGALDLFTALGG
                     TPSPNGSWTYGSAPHNGTYVPGTDQPGNYVYTVSGVAPCSNATATVLVQDVQPPDAGT
                     STTVTRCTSNPSFGLFTALTGNPSTGGTWYQGGQILLDPFFDPGSNSPGSYAYWYVIG
                     GNGDCPADSALLTVTLFQEVNAGCDGNASFCNPSSPAPLFPFLGCSPATGGSWRRPNG
                     QPHSGIFNPATDASGDYWYRKPALGACSADSAKVTVSVVAQHNAGDPGQVTVCSDNPP
                     FLLMNYLTGSPEPGGVWSGPIHAPHSEYYFPQFDASGVYTYTFAASGPCPADSAKVNV
                     IEYQKPWAGNDGVRQVCSDEPGTFALITVLTGGPNPLGSWKKPGLLPHPPGTYDPGNA
                     AFSPPGLYTYVVQGNAPCINDTATVTIIETQAPEAGISTAVQICNSAGVQVLSTLLGG
                     TPGAGGTWTGPGHVSWPSGTFDPSTDPPGLYTYHVNGVFPCGADSATVTIGLVSAPNA
                     GLDGSLSACLNDNNVDLFTGLGGNPQAMGTWTGPNQVNGILHANEVDPGIYTFTYTVS
                     GGGACPVDEALVTVTVSDALFAGDDSEGELCSGMDAALFGFLDGAAQPGGAWTQENGP
                     PAIDVNGVFHPSVTGPGTFNFTYVLAGSPNCPGDTASLSLVVIQGPNAGTGPNNPAQL
                     CTVQPQFNMINLLSGLPDTDGAWFNEVGDPVSAVFDPATSAQGTYTYVVPGAGGCDDD
                     SATVTIQLTTAPDAGATVTHTFCSNDAQTDLFSLLGGTPSPGGSWSYLNTSHSNVYVP
                     LVDNPGTYYYTVSGSFPCSNDTAAIIVSEAVAPYAGQSNTITRCSSQGPFDMRVNLVD
                     PPPPSSFGSWTGPNGAHGATFDPAVDSSGVYTYVVTGDAACAPASATLTVSITYAPDA
                     GNDSTLAVCNTTSDVDLFAALGADADSGGTWTDVSGVGAALQGGIFDATQVGVGGYQF
                     QYNVPANGVCAGDQNTLTVNVGAGLDPGIGDTLTICGGDAAYNMFDALDGTPSPGGVW
                     SSGIIPGVLTGEILDATLLAPGGPYPFSYTVTDPACGEAFSVVLLSVAAYPDPGADTT
                     VTFCSTMTNPFGLLNLLGGDPDPGGTWTTLGGVPVNPPIFTPGTDVAGGFRYQLTATP
                     PCSDTSAVLTIIVNEPPLAGPDTTILVCNSDSLDLDSVLVPGAQPGGAWSDNSGSGWL
                     TGSLVRLDSLDAGIYAYTYQLEVPACPSDMATYTLHVAEGVHVTDTVLVCDEVHRMYT
                     VTLTIAGGDPATYAVTGLEGAIAPGPPYVFTSIPIITSQPFSITVTDANNCGPRVVEG
                     VSPCRFIDEVLVPESFTPNGDNVNDVLIIPGIEGFPRNTISIFNRWGAEVYKAAGYDN
                     RSVVWKGVSDNAMIPGDLPTGTYYYVLELGNGVEPFKGFIYLNR"
BASE COUNT         1122 a         1834 c         1907 g         1086 t
ORIGIN      
        1 tgataccgcc cttcaagccg aatgacagcc gcgcgtcctg gccggcccgg atgcggtacg
       61 ccacatcgag gaacgcgcag ttctgcttcg ccggcccggc cacatcgtgg atcagcgttc
      121 cgcccagcga aagcgtacgg cccgggagtg gcgaatgaac ggtcagggtc tgtgtggacg
      181 gtgcgcccgc gaacccgacc cactgatgtc gactcaggat catggccgaa agcacgtccg
      241 cactgcccgc gtacgccgga ttgaaggcca gcgtattgaa catgtactgg ctgtacatcg
      301 gatcctgctg cgcacgtgag gtcgtggccg acagccaggc ggcaaggacg atcacgatat
      361 gtcgaccggt cctcatcggt tcaggtagat gaagcctttg aacggttcaa ccccattgcc
      421 gagctccagt acgtagtagt aggtgcccgt gggcaggtcg ccgggtatca tcgcgttgtc
      481 cgaaaccccc ttccagacaa cactgcggtt atcgtaaccg gccgctttgt acacttcggc
      541 gccccaacgg ttgaagatgc tgatcgtgtt gcgcgggaaa ccctcgatgc ccgggatgat
      601 cagtacatca ttcacattgt cgccattggg cgtgaaggac tcgggcacca ggacctcatc
      661 gatgaatcgg cagggcgaca cgccttccac cacgcgcggc ccgcagttgt tcgcatcggt
      721 caccgtgatg ctgaacggtt ggctggtgat gatggggatg ctggtgaaca cgtagggcgg
      781 acccggtgcg atcgcgcctt ccagtccggt cacggcgtac gtggccgggt cgccgccagc
      841 gatggtgagc gtgacggtgt acatcctgtg gacctcatcg cacacgagta cggtatccgt
      901 cacatgtaca ccttcggcaa catggagcgt gtacgtggcc atgtccgaag ggcacgcggg
      961 cacctcaagc tgataggtgt acgcgtaaat gcccgcatcc aagctgtcga ggcggaccag
     1021 tgagccggtg agccatccgg agccgctgtt gtccgaccat gcgcctcccg gctgcgcgcc
     1081 cggcaccagc acgctgtcca ggtcgaggct gtcgctgttg cagaccagga tcgtcgtgtc
     1141 cggtccggcg agcggtggtt cgttcacgat gatcgtgagc actgcactgg tatccgaaca
     1201 cggcggagta gcggtgagct ggtagcggaa accaccagcc acatccgttc caggtgtgaa
     1261 gatgggcggg ttcaccggta caccacccaa cgtggtccac gtgccgcccg gatccggatc
     1321 gccgccaagc aggttgagca gaccgaaggg gttggtcatg gtactgcaga acgtcaccgt
     1381 cgtatcggca ccggggtcgg ggtacgcggc cacggaaagc agcaccacgc tgaacgcttc
     1441 gccacaggcc ggatcggtca ccgtgtaact gaatggatag ggcccaccgg gcgcgagcaa
     1501 ggtcgcatcc aggatctccc cggtgagcac ccccggaatg atacccgaac tccatactcc
     1561 tcccggggaa ggcgtgccgt ccagcgcgtc gaacatgttg tacgccgcat ccccgccaca
     1621 gatggtcagt gtatccccga tgcccggatc aagacccgcg ccaacattca ccgtgagcgt
     1681 gttctggtcc ccagcgcata ccccgttggc cggcacgttg tactggaact gatagcctcc
     1741 aacgccgacc tgtgtggcat cgaagatccc gccctgcaaa gcggcaccaa ccccgctcac
     1801 atcggtccac gtaccaccgc tatcggcatc cgctcccagc gcggcgaaga ggtcaacgtc
     1861 gctggtcgtg ttgcacacgg cgagcgtact gtcattaccg gcatcgggcg catatgtgat
     1921 cgatacggtg agtgtggcgg aagccggtgc gcatgccgca tcgccggtga ccacatatgt
     1981 gtacacgccc gagctgtcca cggccggatc aaaggtggcc ccgtgcgccc cgttcggtcc
     2041 ggtccaggaa ccgaaagagg agggtggtgg tggatcgacc agattcacac gcatatcgaa
     2101 tggcccctga ctggaacagc gggtgatcgt gttggattga ccggcatacg gtgccaccgc
     2161 ttccgaaacg atgatcgcgg ccgtatcatt cgagcagggg aatgagccgc tcaccgtgta
     2221 gtagtatgtt cccgggttgt ccaccaaggg cacatacacg ttcgagtggc tcgtgttgag
     2281 gtagctccac gagccacctg gagacggggt accaccgagc aaggagaaca ggtcggtctg
     2341 agcgtcattc gagcagaacg tatgggtgac ggtggcgccg gcatcgggtg cggtggtcag
     2401 ttggatcgtt accgttgccg agtcatcgtc gcatcctccg gccccgggca cgacataggt
     2461 gtaggtcccc tgagcactgg tagccggatc gaacacggcc gataccggat cacccacctc
     2521 gttgaaccag gccccatcgg tatcaggcag gccgctcaac aggttgatca tgttgaactg
     2581 tggctgaacg gtacacaatt gtgccgggtt gttcggaccc gtacccgcat tcgggccttg
     2641 gatcaccacc agggaaagtg atgccgtatc accgggacaa ttgggcgaac cggccagcac
     2701 atacgtgaag ttgaaggtgc ccgggcccgt gacggacgga tggaacactc cgttgacatc
     2761 gatcgccggt gggccgttct cctgggtcca agcgcctccg ggttgggccg cgccatcgag
     2821 gaatccgaac aatgccgcat ccatcccgct gcacaactcc ccttcgctgt cgtcgcccgc
     2881 gaacaacgca tcggacaccg tcacggtgac cagcgcctcg tcgaccgggc acgcacctcc
     2941 cccactcact gtatacgtga aggtatatat tccggggtcc acttcgttgg catggaggat
     3001 cccgttcacc tggttggggc cggtccacgt ccccatggcc tgagggttcc cgcccaatcc
     3061 cgtgaagaga tccacgttgt tgtcgttgag gcatgcgctc aacgaaccat ccaggccggc
     3121 attcggggcg gagaccaggc cgatggtcac cgtggcggaa tcggccccgc acgggaacac
     3181 cccgttcacg tgataggtgt acaggcccgg tggatcggtc gaggggtcga aggtcccgga
     3241 cggccaggag acatggcccg ggccggtcca tgttcccccg gcaccagggg taccgcccag
     3301 cagtgtgctg agcacctgta cgcccgcgga attgcagatc tgcaccgccg tgctgatccc
     3361 cgcttcgggc gcttgcgtct cgatgatggt gaccgtggcg gtatcattta tgcacggcgc
     3421 gttcccctgt accacgtacg tgtacagacc cgggggcgag aaggccgcgt taccagggtc
     3481 gtatgtcccg ggaggatggg gcaacagtcc cggcttcttc caactgccca acgggttggg
     3541 accgccggtg agcacggtga tcaatgcgaa ggtgcccggt tcatcgctgc atacctgccg
     3601 cacaccgtcg ttcccggccc atggtttctg atactcgatc acgttgacct tggccgaatc
     3661 agcggggcac gggcccgagg cggcgaacgt ataggtgtac acgcctgaag cgtcgaactg
     3721 tgggaaatag tattcggaat gaggtgcatg tatcgggcca ctccagaccc caccgggttc
     3781 aggacttccg gtcaggtagt tcatcagcag gaacggagga ttgtcgctgc acaccgtgac
     3841 ctgtccgggg tcgcctgcat tgtgctgagc caccaccgac acggtaacct tggcactgtc
     3901 cgccgagcag gccccgagcg ccggcttccg gtaccaataa tcgccactgg cgtccgtagc
     3961 cgggttgaaa atgccggaat gcggctggcc attgggcctg cgccaggaac ctcctgtggc
     4021 cggcgaacaa cccaagaacg ggaacagcgg tgccggggac gaagggttgc agaacgaggc
     4081 attcccatcg cacccggcat tcacttcctg gaaaagcgtc acggtgagga gcgcggaatc
     4141 cgccggacaa tccccattgc caccgatcac gtaccagtac gcgtacgatc cggggctgtt
     4201 gctccccgga tcgaagaagg gatcgagcag gatctgacca ccttgatacc acgtgcctcc
     4261 ggtcgagggg ttgccggtaa gcgccgtgaa cagcccgaag gatgggttcg aggtgcatct
     4321 ggtcacggtc gtgctcgttc cggcatccgg cggttgcaca tcctgcacca gtacagttgc
     4381 cgtcgcattg gagcaggggg ctaccccact gacggtatac acatagttgc ccggctgatc
     4441 cgttccgggc acgtatgtac cgttatgggg tgcgctgccg tacgtccatg agccattggg
     4501 cgagggtgtt ccgcccaagg cggtgaacag gtccagcgcg ccgcccgtga ggcagtgttg
     4561 cacgatattc gagacacccg catccggcag cggattctcg aaaaccgtta cgcgggcggt
     4621 gtcgttcgga cacggggacg cacccgtgat cacataggtg tacgtatgtc catcggggtc
     4681 gaaaggcaac gtgccgggtg cgtacaaccc acctgtgata gcgccgccct catccttcca
     4741 tgttcctccg ggagcatatg ctccgttcag tacgttggcc agttggaatg aggaactgtt
     4801 ctcgcagacc gtcgtatccc catcgaggcc ggcattggcg gccgggttga ccacgatggt
     4861 cagggcgatc atcgccggcc cgcatggcga agccccgttc accgtatagg tccaggtgcc
     4921 cgcaggatcg acgccgggaa caaacgtgga attgtgtacc gtgttgctgg gatcacgcca
     4981 ggtgccgttc ggtgccggag aacaggtcag gatctgcgtc aggtcgaacg ccggaccatt
     5041 ctggcagcgc gtgatggagc cgggcgaaca accggcattc ggcggggcca cctcctgtac
     5101 ggtgacccgg gcggtatcat tctgacaagg cgaactgttc tgtgtgatgt agaggaagac
     5161 ctgcgggttg tccgtggcag ggttgtagct cccgctgaac ggtccgggag ggacggtctt
     5221 gcgccacgtt ccgggagcct cggctcccgt gaggtacggg aacaacggtg tcgatgacgc
     5281 attcccgcac aggggcacgg tgacatcgtt gccagcgaac agcccgtgcg ccacggtgac
     5341 gctcgccacg gccgaatccg aggcgcaact gagggatccg gtcagcacgt accggaactg
     5401 gtatgtggtt ccagccgtga gcacggtggc gtccaggttc tgacccacca cctgcccgcc
     5461 cggcagggcc ttccagatgc caccgggttg cgggccactg ccaaggcagt tgaagaggtt
     5521 gtacgccggg ttcttgtcgc acacggtaca cgttccattc gtacccgcgt tgagcatgcc
     5581 ggtgatgtgc acgatcacct ccgcatcagc gggcgggcaa ttgccgttgc ccggcaaatt
     5641 gtaccggaag tgatacgttc ccggcgggtt ggtcgcgcag aagaacaccc cgctgttcaa
     5701 ggtgccggga gcgatgctgc cgatggcctg ccaggttccg ccagcatcgg ggctgccacc
     5761 gagaacaggg aacagatcca cctgggacgt gccttcgcat acggtgatat cggcatcctc
     5821 gcccggatcc ggggccgcat tctgcgtcac cgtcatcatg gaagtggcat tcgcgcaggg
     5881 cgtcgtaccg gctaccgtgt acgtgaacac gtgcgtgccg ttggaattgg cagcaggggt
     5941 gtacaggcc
//
LOCUS       DAOQOC010000028         5941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5267_length_5941_cov_3.448012, whole genome shotgun sequence.
ACCESSION   DAOQOC010000028 DAOQOC010000000
VERSION     DAOQOC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5267_length_5941_cov_3.448012"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PL106_00800"
     CDS             <1..434
                     /locus_tag="PL106_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNA31631.1"
                     /translation="AIIAQRDDRPEVPAVLILESGLLSSGLTNTAKELVRRPRPYLYN
                     PAVPASVHRKGEDAVSFWSGHTANMAAITFSAAAIVQRSDVSSDAKLATWLGAAAAPA
                     LMGYLRVRAGRHFPSDVLVGYLVGAAVGLAVPYIHQPRTAH"
     gene            complement(575..862)
                     /locus_tag="PL106_00805"
     CDS             complement(575..862)
                     /locus_tag="PL106_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNA31632.1"
                     /translation="MSTDPIIRRFRTWAIAEGWSFLVLLLVAMPLKYLAEWPLGVKVV
                     GWAHGVLFIGYWITATPLFTKLKWDVERIVGLGLASVLPFGTFVMERKWLR"
     gene            960..1958
                     /locus_tag="PL106_00810"
     CDS             960..1958
                     /locus_tag="PL106_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA31633.1"
                     /translation="MPRVSILLPFHNAATTLDAAIASIATQTFLDWELLLIDNACTDK
                     GTAIAHDRATRDARIRVMQEPVVGIAHALNTGLREARATLIARMDADDISLPERLERQ
                     VAYLDQHPEVGVLGTCTRFRSTVDLNEGMAAFVKWQNTIISPKDHYVKRFVDAPLAHP
                     TVMFRSELVDRHGGYDTGPLPEDHELWLRWMHYGVRFAKLPQELLIWRDHPQRLSRSH
                     SNYAVEAFFSTKVKWLALWLERILNGRPVIVAGTSALCRGRAAQLSAHGIPIHGYTDV
                     KRHVVPGYPFVPHDELPGPGQAFIVSFISQRGTGDRIAAYLVSRGLVEGEDFILAA"
     gene            2048..2662
                     /locus_tag="PL106_00815"
     CDS             2048..2662
                     /locus_tag="PL106_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNA31634.1"
                     /translation="MIDVVVTFAVATVASFIGSLQAGLVNTAVLAATLRHGPASGMRT
                     AWGGCIPEFIYAAVAFTAAERILAWTEGLGGTVDRVAGGVMIVLGVYFLFFLKPFPSA
                     QQLEEKRTGFMNGLLLGLMNPQLLLFWCGVRLALESFEVDVHGWPAILAFGLGAFCGA
                     MILLMMLVRLGRRMQERLGSRALHNIFRLVGALLVATGIVAWLR"
     gene            complement(2712..2945)
                     /locus_tag="PL106_00820"
     CDS             complement(2712..2945)
                     /locus_tag="PL106_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31635.1"
                     /translation="MATLRFHWDFFGPDARGTAEHFLEHVDEFCANEGITGHRHRVME
                     LPGRATASLDCDEKHLALVRDRLRPKRAERFME"
     gene            complement(2945..3937)
                     /locus_tag="PL106_00825"
     CDS             complement(2945..3937)
                     /locus_tag="PL106_00825"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNA31636.1"
                     /translation="MGERIAVIGAGSWGTALVKLLSANTERTGWWVRRPETIAHIRTY
                     GHNPDYISAAQFDVGKLAMDPDVHVVVRDADTLVLAVPSAFLKGVLDELGKEELRGKT
                     IFSAVKGIIPQTNQIAGEYLFEHMGIAEGNFGVICGPCHAEEVALERLSYLTIACQDS
                     DKAKAMGAALNGRFMKTTLSDDIYGTEYAAILKNVIAVCAGISVGLGYGDNFRAVLVS
                     RAIQEIDRFVKAVHPIERDINEPAYLGDLLVTAYSTFSRNREFGTMVGKGYGVKAAQL
                     EMNMVAEGYYAVKCVHEINRKLKADMPIVESTYRMLYEQKSPSVEMRLLSDRLA"
     gene            complement(4027..5742)
                     /locus_tag="PL106_00830"
     CDS             complement(4027..5742)
                     /locus_tag="PL106_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNA31637.1"
                     /translation="MQKPRSLSPLLALMIGSALHAQSQSDSLALANMRADIRYLASDS
                     LHGRETATPYETLASDHIIARFKEAGVRPMGENGGFLQAFTLDADPVRGPLNSARIGR
                     YTLTLDVDFYPVSWSSSTSVLTRLAKCRYGIKAPEKNRDDFADVDVKDHAAAISIGSP
                     DGIHPHSEWIDHQDIGQRIKDAVSLGANAVILYNDDPTATDPDSTLSKKVHACGVPVI
                     FLTKEGQKKMGLDGEPVVISTDIIRRVLSGHNVIGMVDNGRPNMVVIGAHYDHLGMGI
                     EGSLHRGGTDIHNGADDNASGTAMMLQLARDLQQMPGAKNNDYLFMAFSGEEMGLLGS
                     NWWTKHPTAPLEELNYMINLDMVGRLDSLNTVIINGVGTSPAWNKLRPDTTVAKKPRR
                     SKKEAPPMDALKVKTTESGVGPSDHTSFYLKDIPAIHYFTGAHDDYHKPSDDEARINY
                     DGMLRVLHHIEDLIVSLNDSGKVAFTKTAVDTNATPTFKVTLGVVPDYAYSGEGMRID
                     GVTDGKPAAKAGLKAGDVVLQLGEHRVTDMMSYMRGLGHFTKGEETLVTVLREGKEVK
                     VKVTW"
BASE COUNT         1033 a         1865 c         1830 g         1213 t
ORIGIN      
        1 ccgccatcat cgcccaacgc gatgatcggc cagaagtacc ggcggtattg atactcgaga
       61 gcggcctgct cagttccggg ttgaccaata cggcgaagga actggtcaga agaccgcggc
      121 cgtacctgta caacccggcg gtaccggcca gcgtccatcg caagggcgag gatgcggtga
      181 gtttctggag tggccacacg gcgaacatgg ctgccatcac cttcagtgcc gcagcgatcg
      241 tgcagcgctc cgacgtttca tcggatgcga aactggccac atggctcggt gccgctgccg
      301 cccccgcgct catgggctat ctccgcgtgc gtgccggacg tcatttccca tcggacgtgc
      361 tcgtgggtta cctggtcggt gccgcggtgg gactcgcggt cccctatatc catcagccca
      421 ggaccgcaca ttgacccggc aggtgcgcac catgcgggac gaagcatacc ttgtactgtt
      481 cgtacggtat tgaacatcat gatgatgcga ctccggtacg ggaacgcgcg ccatcaccgc
      541 cgatccgtgc cgggaaccgg acatgaaccg gggatcagcg cagccacttc cgctccatca
      601 cgaaggtgcc gaaaggaagc accgaggcca ggcccaaacc aacgatccgc tccacgtccc
      661 atttgagctt ggtgaagagc ggggtggcgg tgatccagta gccgatgaag agcacgccgt
      721 gggcccagcc caccaccttc acacccaagg gccattcggc caggtatttc aagggcatgg
      781 caacaagaag caggacgagg aaactccatc cttcggctat ggcccaggtg cggaagcggc
      841 ggatgatggg atcggtggac atggggcacg aatgtacccg gcacatcgaa cgtggtgcgc
      901 ttcaaccgga tgcgtcgtta cggtcgcgcc cgaacttcgc cgagccaact cactgagcga
      961 tgccgcgcgt ttccatcctg ctccctttcc acaacgcggc caccacgctc gatgccgcaa
     1021 tcgccagcat cgctacgcag accttcctgg actgggaact cctgttgatc gataacgcgt
     1081 gcacggataa gggtacggcc atcgcgcacg accgggcaac acgggatgcg cgtatccgtg
     1141 tgatgcagga accagtcgtt ggtatcgctc acgcattgaa caccggcctg cgcgaagcgc
     1201 gagccacgct catcgcccgc atggatgccg acgatatctc ccttccggag cggctggaac
     1261 gacaggtcgc gtacctggat caacatccgg aggtcggtgt gttgggcaca tgcacgcgct
     1321 tccgatcgac cgtggacctg aacgagggta tggccgcatt cgtcaagtgg cagaacacga
     1381 tcatatcgcc gaaggatcat tatgtgaagc gcttcgtgga tgcgccgctt gcccatccca
     1441 ccgtgatgtt ccgcagcgaa ctggtggatc gccacggtgg ctacgacacc ggccccttgc
     1501 ccgaggacca tgaattgtgg ttgcgctgga tgcactacgg cgtgcgtttc gcgaagctgc
     1561 cgcaggagtt gctgatctgg cgggatcatc cacaacgcct gagccgatcg cacagcaact
     1621 atgcggtgga agcgttcttc tccacgaagg tgaaatggtt ggccctgtgg ttggagcgca
     1681 tcctgaacgg acggcctgtg atcgttgcgg gaaccagcgc gctctgtcgg ggtcgggccg
     1741 cacaactgtc cgcgcacggg atccccatcc acgggtacac cgatgtgaag cgacatgtgg
     1801 ttccgggata tcccttcgtc ccgcatgatg agctgcccgg accaggacaa gcattcatcg
     1861 tctccttcat cagccagcgc ggcacagggg atcgcatcgc agcgtacctg gtctcgcgcg
     1921 gactggtcga aggcgaggac ttcatactgg cggcgtaacc gctgttgagc ggtcgggccg
     1981 ggctgttgca catgctgcgt ggcccctact tttcacgcgg ggagccgaac acccttcgtg
     2041 cgatgcgatg atcgatgtgg tagtgacctt cgctgtggcc accgtggcca gcttcatcgg
     2101 ttcgctgcaa gcgggtctgg tgaacaccgc cgtgctggcc gccacactcc ggcatggacc
     2161 ggcatcgggc atgcgcaccg cgtggggcgg ctgcatcccg gagttcatct atgcggccgt
     2221 tgcgttcacg gccgccgaac ggatcctcgc gtggaccgaa ggccttggtg gtaccgtgga
     2281 ccgcgtggcc ggcggggtga tgatcgtgct gggcgtgtac ttcctgttct tcctgaagcc
     2341 cttcccttcc gcacaacaac tggaggagaa gcgcaccggg ttcatgaacg gcctgctgct
     2401 cggactgatg aacccgcaac tgttgctgtt ctggtgcggc gtgcggctcg cattggaaag
     2461 cttcgaggtg gacgtgcacg gatggcccgc gatcctcgca ttcgggctcg gggccttctg
     2521 tggtgccatg atcctgctga tgatgctggt gcggctggga cggcgtatgc aggaacgcct
     2581 cggcagccgg gccttgcaca acatcttccg gttggtgggt gccctgctgg tggctaccgg
     2641 tatcgtagcg tggctgcggt gatcccgttg tgctccgctg agccgtggag ggcgctgctt
     2701 cgcagcatga cctattccat gaagcgctcg gcacgcttcg gccgcaaccg atcgcgaacc
     2761 agcgccaggt gcttttcatc gcaatcgagc gatgccgtgg cgcgtccggg cagttccatc
     2821 acccggtgcc ggtggcccgt gatcccttca ttcgcacaga attcatccac gtgctccagg
     2881 aagtgctccg cggtgcccct ggcatccggg ccgaaaaaat cccaatggaa gcggagcgtt
     2941 gccatcacgc cagccggtca ctcagcagcc gcatctccac ggagggcgac ttctgttcgt
     3001 acaacatgcg ataggtgctc tccacgatgg gcatgtccgc cttgagtttg cggttgatct
     3061 catggacgca cttcaccgcg tagtagccct cggccaccat gttcatctcc aactgggcgg
     3121 ccttcacgcc gtatccctta cccaccatgg tgccgaactc gcggttgcgg ctgaaggtgg
     3181 aatacgcggt cacgagcaga tcgccgaggt aggccggttc gttgatgtcg cgttcgatgg
     3241 gatgaacggc cttcacgaag cggtcgatct cctggatggc ccggctcacc agcaccgccc
     3301 ggaagttgtc gccgtagccc aaccccacgc tgatgcccgc gcacaccgcg atcacgttct
     3361 tgaggatggc cgcgtactcg gtgccgtaga tgtcgtcgct gagcgtggtc ttcatgaacc
     3421 gcccgttgag cgccgcaccc atggccttgg ccttgtcgct gtcctggcag gcgatggtga
     3481 ggtagctgag ccgctccaac gccacctctt cggcgtggca cggcccgcag atcaccccga
     3541 agttaccctc ggcgatcccc atgtgctcga aaagatactc gccggcgatc tggttcgttt
     3601 gcggaatgat ccccttcacc gcgctaaaga tggtcttccc gcgcagctcc tccttgccga
     3661 gctcgtccag tacgcccttg aggaaggcac tgggcacggc cagcaccagc gtgtccgcat
     3721 cgcgcaccac gacgtggaca tccggatcca tggccagttt ccccacatcg aactgcgcgg
     3781 cactgatgta gtcgggattg tggccgtagg tgcgtatgtg cgcgatggtc tcggggcggc
     3841 gtacccacca gccggtacgt tcggtgttgg ccgaaagcag tttgaccagg gccgtgcccc
     3901 aactgcccgc tccgatcacg gcgatccgtt cacccatgcg gtggctagtt tacgatgaca
     3961 ctgtgaatgg gaccggcttg cattccgcgc acctgtcttt ccgggtatcc ggtcgaggat
     4021 cgcacatcac caggtcacct tcaccttcac ttcttttcct tcgcgcagca cggtcaccag
     4081 ggtctcctct cctttggtga aatgacccag gcccctcatg taactcatca tgtcggtaac
     4141 gcgatgctcc ccgagttgca aaacgacatc cccggccttc agacccgctt ttgcggcggg
     4201 tttgccgtcg gtgacgccat caatgcgcat gccttcgccg ctgtacgcgt aatcaggtac
     4261 aacccccagg gtgaccttga aggtgggggt ggcgttcgtg tccaccgcgg tcttcgtgaa
     4321 cgcgaccttg ccactgtcgt tcagcgaaac gatcaggtcc tctatgtggt gcaacacgcg
     4381 gagcatgccg tcgtagttga tgcgagcctc gtcgtcgctg ggcttgtggt agtcgtcgtg
     4441 cgcaccggtg aagtagtgga tggccggaat gtccttcagg tagaagctcg tgtggtcgct
     4501 cggcccaaca ccgctctccg tggtcttcac cttcaacgca tccatgggcg gggcttcttt
     4561 cttcgaccgc cggggcttct tcgcgaccgt cgtatccggc cgcagcttgt tccatgcggg
     4621 cgaagtgccc acaccgttga tgatcaccgt gttcaggctg tccagacgcc ccaccatatc
     4681 caggttgatc atgtagttca attcttccag aggtgcagta ggatgcttcg tccaccagtt
     4741 gctgcccagc agtcccatct cctctccgct gaaagccatg aagaggtagt cattgttctt
     4801 cgcgccgggc atttgttgga gatcgcgggc gagctgcagc atcatggccg tgccgctggc
     4861 gttgtcgtcg gccccattgt gtatgtcggt accaccccga tgcagactgc cttcgatgcc
     4921 catgcccagg tggtcgtagt gcgcgccgat gaccaccatg ttcggacggc cgttgtccac
     4981 catgccgatc acgttgtgtc cgctgagcac ccgtctgatg atatcggtgc tgatgaccac
     5041 gggctcgcca tcaagcccca ttttcttttg tccttccttg gtcaggaaga tcaccggaac
     5101 gccacaagca tgcaccttct tgctcaaggt gctgtccgga tcggtggccg tgggatcgtc
     5161 gttgtacagg atcaccgcat tcgctccaag ggagaccgca tctttgatcc gctggccgat
     5221 gtcctggtga tcgatccact cgctgtgcgg gtggatgccg tccggcgaac cgatcgagat
     5281 ggccgctgcg tggtccttca catccacatc cgcgaagtcg tcccggttct tctccggcgc
     5341 cttgatgcca tagcggcact tggccaagcg ggtgagtacc gaggtggagg atgaccagct
     5401 caccggatag aagtcgacgt ccaaggtcaa cgtgtaacgc ccgatccgcg cggagttcaa
     5461 cgggccgcgc acggggtcgg catccagggt gaaggcttgc aggaagcccc cgttctcccc
     5521 catggggcgc actccggctt ccttgaagcg tgcaatgatg tggtccgaag ccagcgtctc
     5581 gtacggcgtg gcggtctccc ggccgtgaag gctgtcgctg gccaggtaac ggatgtcggc
     5641 gcgcatgttg gccagggcaa gtgagtcgga ctgcgattgt gcgtgaagtg cgctgccgat
     5701 catcagcgcg agaagcgggg aaagtgaccg tggtttttgc atgggcctgc gaagatatcc
     5761 cgacagggcc gtgccggaac cggggcgatc ctgcccactt tgcgcccatg agatccatct
     5821 tgtacggcat tttgcttttg gccgcgtgcg ggactcccgt ggttgatggg ggctccgccc
     5881 catacggccc tggttcatcc actgcgaaga ccgactggcc gatcatcgac acgcaggatc
     5941 a
//
LOCUS       DAOQOC010000029         5911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5310_length_5911_cov_4.172131, whole genome shotgun sequence.
ACCESSION   DAOQOC010000029 DAOQOC010000000
VERSION     DAOQOC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5310_length_5911_cov_4.172131"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5437)
                     /locus_tag="PL106_00835"
     CDS             complement(<1..5437)
                     /locus_tag="PL106_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNA31638.1"
                     /translation="MGNLDQVALSIPSTHGSMVLDLYRADVVTEEFVVRAASTRSPVE
                     MPTSIHYRGAVRGVAGSVVSVSVFQEEAMALISDASGERVVGRFGRGPEDLLVFYHTD
                     ELLGSAHTSCATSDHGNAEVQGGERAQSPTTLRCVRWHWEVSFDIFQDKGSVTNAANY
                     ATALFNQSATLFANDGISVALQEVVVWDVPDPFGGGNSEEVLQTFQDHTGSINGDMGH
                     LLDLDMLNGGIAANIGTLCETQVDERLCYSGIESTFNNVPVFSWSVNVVTHEQGHLLG
                     SWHTHACVWNGNNTAIDGCSTVEGGCPQPPCPPTGTIMSYNSNIDFALGFGPQPAQVI
                     RDHVNSSSCLFTCGGTACDSIFVALATPAAASTVLHLSWADVPFPAMVGEDFALCLND
                     GCFSGLLTDPNTTSVDGAFTVTDADGDTIVSGTTTGGQFAFTLGTPVLGCTDPEADNF
                     DPDATCDDGTCCSTLLTLALTDSAGDGWEGAMYALVNVLGDTAFSGTLASGAGDTLTF
                     CPPVGCYVFTVTSGTDPAEISWALSGDVFLSGDANSSITMSLGGAITGCMDTLACDYN
                     PMATCPGTCSYGEAATIIIVPSTTPNDVSWALLDPDGLGVASGNGFASSFTTCLPPAC
                     GYRMFMTDGAGEGWDQGFYAVIIGTDTVAGTLEEGSFGVDTLNFGPQAGCMDTAACDY
                     DPLATCPQVCAATPTWFADADGDGYGDLVADSVACTAPVGFVSNAFDCDDTQPAVNPF
                     AVEICDGLDNDCDGAVDDGFIWYVDADADGFGDDATMQVSCTPLPGGVQQGGDCDDTS
                     ALITAPGEECDDGDPNTGNDTMNAFCVCVGQPVGPCPPGEIPDCNGNCAPAEWVGDGF
                     CDNGTFEYNGAIIFFNCAAFNFDEGDCAGCVTEICDGIDNDCDGEIDEGFVEVCNGLD
                     DDCDGVVDEGIAWMDADGDGFGDVNAPVICTATGAVANSLDCDDADDGLAYGITLGVF
                     TDEVDVAGTAHYTVTQGSTVLEGDIDLIAAEGGFGSVELCLGAGCFTVQVVEVDEPLF
                     VESYIGFAHAPNNTVAFPTAQGFQGQASGQSTEICNGIDDDCDGQMDEGSPQEYADSD
                     GDGYGDPLQPLPCDTPGVANNLDCNDDDPAINPDQGCGNCSATHRAWITQNQSTIEDI
                     VATNLNTCAGSGDLVACLTDLIVQETPLPQACAACIAQRYACILNTCMVPCLGGFGTP
                     GCQSCVQANCNAAYFACAGFTDADADGVVAEADCDDNNAAIYSGAAELCDGIDNDCDG
                     STDEGVVITWYTDADFDGYGDDATAIIGDCDQPPGMVTDGGDCDDTDQTVFPGAPELC
                     DGIDNDCNGAVDDNAGLAYYVDADNDLFGDDGTETFSCTPIPGMITQGGDCDDADAAV
                     HPGASDPCDNIDQDCSGGPFLTVWYQDNDGDTYGNDAVFTQACAQPPGFVAAGGDCDD
                     ANANVFPGNGCGNCTGTDQSWLVTHQEELLNTMGACAGQCFDDPGCLVLCMQGQGIPL
                     SALCLSCVPEHVTCLFDNCTFACINSPEECLQCQVQSGCVAELSDCMGMADGDGDGWW
                     TGSDCDDSNAAVYPGALETCDGLDNDCDGGIDEGISFTWYTDADGDGYGLDGTGVPGD
                     CDQPPGTAGQTGDCDDANDAVFPGAGELCDNIDNDCDGEVDNGAGEFYYTDADEDGFG
                     DEASAVLSCTPVPNTITTGGDCDDNDATIHPGAADPCDGIDQDCSGGPVITTWYQDLD
                     NDDFGNTNVTLNDCVRPVGYVALNGDCDDTNANLFPGNGCGNCAPTEQAWLADHQEEL
                     LAALGTCMVQCLGDAGCISSCMMNEGIP"
     gene            5393..>5911
                     /locus_tag="PL106_00840"
     CDS             5393..>5911
                     /locus_tag="PL106_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31639.1"
                     /translation="MGRRNAQRDLIEVSQDQLVERNLIIGGLSGREDFLAPRNRFFGG
                     ALGRRLINLHLSPRSHLFLPSLNQGIDKLQESLSGIAFRNDHRPALIFQSVAISSRVL
                     ERLWFGALICILLRRQCVARKLCFQGLRSFLCITEQQIQSNLITAGHAHCATPSALGI
                     TPCAWVTSSPCGV"
BASE COUNT         1050 a         1934 c         1784 g         1143 t
ORIGIN      
        1 cggggatgcc ttcgttcatc atgcagctgg aaatgcaccc cgcatcaccc aggcattgca
       61 ccatgcatgt gcccagggcg gcgagcagtt cttcctgatg atcagcaagc catgcctgtt
      121 cagtgggcgc gcaattgccg cagccgttgc cggggaatag gttcgcgttg gtgtcgtcgc
      181 agtcgccgtt cagcgcgacg tagccaacag gtcgcacaca gtcattcagc gtcacgttcg
      241 tgttaccgaa gtcgtcattg tccagatcct ggtaccacgt ggtgatgacc ggcccgccgc
      301 tgcaatcctg gtcgatgccg tcgcacgggt cggccgcgcc ggggtggatc gtcgcatcgt
      361 tgtcgtcgca atcgccgcct gtggtgatag tgttcggcac aggtgtgcag gagagcacgg
      421 cgctcgcctc gtcgccgaag ccatcttcat cggcgtccgt gtagtagaac tcgccggctc
      481 cattgtccac ttcaccatcg cagtcgttgt cgatgttgtc gcagagctcc cccgcaccgg
      541 ggaataccgc gtcgttcgca tcgtcacagt cgccggtctg gcccgccgta ccgggcggct
      601 ggtcgcaatc ccccggcacg cccgtgccat ccaggccgta gccatcacca tccgcatcgg
      661 tgtaccaggt gaagctgatg ccttcatcga taccgccgtc acagtcgttg tccaggccgt
      721 cgcacgtttc cagcgccccg ggatagacgg ccgcgttgga gtcgtcacaa tcgctgccgg
      781 tccaccagcc gtcgccatcg ccatcggcca tgcccatgca atcgctgagc tccgccacgc
      841 aaccggactg tacctggcat tgcaggcatt cctccggtga gttgatgcac gcgaaggtgc
      901 agttgtcgaa gagacaggtc acgtgttcgg gcacgcacga caggcagagg gcgctgagcg
      961 gaatgccctg tccctgcatg cagaggacca ggcatccggg atcatcgaag cattggccgg
     1021 cgcaagcccc catcgtgttg agcagttcct cctgatgggt gaccagccag ctctgatcgg
     1081 tacccgtgca attgccgcag ccgttgccgg ggaacacgtt cgcgttcgca tcatcacaat
     1141 caccaccagc ggcgacaaag cccggtggtt gcgcgcaggc ctgtgtgaac accgcatcgt
     1201 tgccataggt gtcgccatcg ttgtcctggt accagacggt gaggaacgga cctccgctgc
     1261 aatcctggtc gatgttgtcg caagggtccg atgcgccggg atggacggcc gcatcggcgt
     1321 catcgcaatc accgccttgt gtgatcatgc ccgggatggg cgtacacgag aaggtctccg
     1381 tgccatcatc accgaacagg tcgttgtccg cgtcgacgta gtaggcgagg cccgcgttgt
     1441 cgtcaacggc accgttgcaa tcgttgtcga tgccgtcgca gagctcgggc gcgccgggga
     1501 agaccgtctg gtccgtgtca tcgcaatcgc cgccgtcggt caccatgccg gggggctggt
     1561 cgcagtcgcc gatgatggcc gtcgcatcat cgccgtagcc gtcgaagtcg gcatcggtgt
     1621 accatgtgat gaccacgcct tcatcagtgg agccgtcaca gtcgttgtcg atgccatcgc
     1681 acagttccgc ggcgccggaa tagatggccg cgttgttgtc gtcgcagtcg gcttcggcca
     1741 ccacgccatc cgcatccgca tcggtgaagc ccgcgcaggc gaagtaggcc gcgttgcaat
     1801 tggcttgcac gcaactctga cagcccggcg ttccgaaccc gcccaggcac ggcaccatgc
     1861 aggtgttcag gatgcacgca taccgctgcg cgatgcacgc tgcgcaggcc tgcggcaacg
     1921 gggtctcttg cacgatgagg tcggtgaggc aggccaccag gtcaccggat cccgcgcatg
     1981 tgttcaggtt cgtggccacg atgtcctcga tggtgctctg gttctgtgtg atccacgcgc
     2041 ggtgcgtcgc ggagcagttg ccgcaccctt gatcaggatt gatggccggg tcgtcgtcgt
     2101 tgcaatcgag gttgttggca acaccgggcg tgtcgcaggg aagcggttgt agtggatcgc
     2161 catagccatc accgtcgctg tccgcgtact cctgtgggga tccttcatcc atctgcccgt
     2221 cgcaatcatc gtcgatgccg ttgcagatct cggtgctctg gccggaagcc tgcccttgaa
     2281 agccctgggc cgtggggaag gcgaccgtgt tgttgggcgc atgcgcgaac ccgatgtagc
     2341 tctcgacgaa gagcggctca tccacctcga ccacctgtac ggtgaagcac cctgcaccca
     2401 ggcacagttc cacggatccg aaacctccct ctgcggcgat caggtcgatg tcgccctcga
     2461 gcacagtgct gccttgggta acggtgtagt gcgccgtacc agcgacgtcc acttcgtccg
     2521 tgaacacgcc caaggtgatg ccgtaggcta ggccatcgtc cgcatcgtcg caatccaggc
     2581 tgttggccac cgcgcccgtt gcggtgcaga tcaccggtgc gttcacatcg ccgaaaccgt
     2641 cgccatcggc atccatccac gcgatgccct cgtccaccac accatcgcaa tcatcatcca
     2701 ggccgttaca aacttccacg aagccctcgt cgatctcgcc atcgcaatcg ttgtcgatgc
     2761 cgtcgcagat ctcggtgacg catccagcgc aatcgccctc atcgaagttg aacgcggcgc
     2821 agttgaagaa gatgatcgcc ccgttgtact cgaacgtgcc gttgtcgcag aagccatcgc
     2881 ccacccactc ggcaggcgcg cagttgccgt tgcagtccgg gatctcgccg ggcggacacg
     2941 gacccacggg ctggccgaca caaacacaaa aggcgttcat cgtgtcgttg cccgtgttcg
     3001 gatcaccatc atcgcactcc tcaccgggcg cggtgataag cgcgctggtg tcgtcgcaat
     3061 caccgccttg ctgcacaccg ccgggcaatg gggtacagga gacctgcatc gtcgcatcat
     3121 cgccgaagcc gtccgcatcg gcatccacgt accagatgaa gccgtcgtcc accgcgccat
     3181 cgcagtcgtt gtccagacca tcacagatct ccacggcgaa ggggttcacc gcgggctggg
     3241 tgtcgtcgca atcgaaggcg ttggatacga agcccacggg agcggtgcag gccacggagt
     3301 ccgcaacgag gtcgccgtat ccatcaccgt cggcatccgc gaaccaggtg ggtgttgccg
     3361 cacagacctg gggacaggtg gccaacggat cgtagtcgca cgccgcggta tccatgcagc
     3421 ccgcttgcgg cccgaaattg agcgtgtcca ccccgaatga tccttcttcc agggttcccg
     3481 cgacggtgtc cgtgccgatg atcacggcgt agaagccctg atcccagccc tcgcccgcgc
     3541 cgtcggtcat gaacatgcgg tagccgcatg cgggagggag gcaggtggtg aaactgctgg
     3601 cgaagccgtt tcctgaagcc actccgaggc cgtccgggtc caacagcgcc cagctcacat
     3661 cattcggggt ggtgctgggc acgatgatga tcgtggccgc ttcgccatag ctacaggtgc
     3721 ctggacaggt cgccatcggg ttgtagtcgc acgcgagcgt gtccatgcac ccggtgatgg
     3781 ccccgcccaa actcattgtg atggagctgt tcgcgtcgcc cgacaggaac acgtctccgc
     3841 tcaaggccca actgatctcc gcagggtccg tcccggaggt taccgtgaac acgtagcaac
     3901 cgacaggtgg gcagaaggtg agcgtatcac cggcgccgga cgccagggtg ccggagaacg
     3961 ccgtatcacc gagcacattc accaaggcgt acatcgcgcc ttcccaccca tcgcccgcac
     4021 tgtcggtgag ggccagcgtg agcaaggtgc tgcaacaggt gccgtcatcg caggtggcgt
     4081 ccggatcgaa gttgtccgcc tccggatccg tgcaacccag taccggcgtg cccagggtga
     4141 atgcgaattg tccccccgtg gtggtacctg aaacgatggt gtccccgtcc gcatccgtca
     4201 ccgtgaaggc gccgtcaacc gatgtggtgt tcgggtcggt gagcaggccg ctgaaacagc
     4261 cgtcgttcag gcacagggcg aagtcctcgc caaccatcgc cgggaagggt acgtctgccc
     4321 acgacagatg cagcaccgtg ctcgctgcgg ccggggtggc caacgcaacg aagatcgagt
     4381 cgcacgccgt gccaccacag gtgaacaaac aggacgatga attgacatgg tcgcggatca
     4441 cctgtgcggg ctgcgggccg aagcccagcg cgaagtcgat gttgctgtta taactcatga
     4501 tcgtgccggt cggtggacac ggtggctgtg ggcagccccc ttccacggtt gagcatccgt
     4561 cgatcgcggt attgttcccg ttccacacgc aggcatgggt atgccaggag cccaaaaggt
     4621 gtccctgctc gtgcgtcacc acgttcacgc tccaactaaa cacgggcaca ttgttgaagg
     4681 tgctttcgat gccgctgtaa cagagacgct catccacctg cgtttcacag agcgtgccga
     4741 tgttcgcggc gatcccgccg ttcagcatat ccaggtccag caggtgaccc atatcgccat
     4801 tgatcgaacc ggtgtggtcc tggaacgtct gcagcacttc ttccgaattg ccgccgccga
     4861 acggatccgg aacatcccag accaccacct cctgcaacgc cacgctgatg ccgtcgttgg
     4921 cgaagagcgt tgcgctctgg ttgaagagcg ccgtggcgta gttcgccgcg ttcgtcacgc
     4981 ttcccttgtc ctggaagatg tcgaaggaca cttcccaatg ccaacgcacg catcgcagcg
     5041 tggtgggcga ctgcgcccgt tcgccgccct gcacctcggc gttcccatgg tcgctggtgg
     5101 cgcatgatgt gtgcgcggac ccgagcagct cgtccgtatg gtagaacacg agcagatcct
     5161 caggcccccg accgaagcgg ccaacgacgc gttcgccgct ggcgtcggag atcagcgcca
     5221 tcgcctcttc ctggaatacg cttaccgaaa ccaccgaccc ggcaacgccg cgtacggctc
     5281 ctcggtagtg gatgctcgtc ggcatttcaa ccgggctacg cgtgctcgcg gcacgcacca
     5341 cgaactcttc cgtgaccaca tccgcccggt acaggtccag caccatcgat ccgtgggtcg
     5401 acggaatgct cagcgcgacc tgatcgaggt ttcccaagat caactggtag agcggaatct
     5461 catcatcggt ggtctgtccg gtcgcgaaga ctttcttgcg ccacgcaatc gcttcttcgg
     5521 cggtgcactt ggccggcgcc tgataaatct tcatctgtcc ccacgatccc atctgttctt
     5581 gccgtcgctg aatcagggga tcgacaagct ccaggagtcg ctttccggca tcgccttccg
     5641 gaatgatcac cgcccagccc tgatcttcca gagcgttgcg atctcctccc gcgttcttga
     5701 gcgactctgg tttggggcgc tgatctgcat tctgctgcgc agacagtgtg tcgcgcgcaa
     5761 gctctgcttt cagggactcc gttcgttcct ctgcatcaca gagcagcaga ttcagtccaa
     5821 cctcatcact gctggacacg ctcattgtgc gactccttcg gctttgggaa tcacgccgtg
     5881 cgcatgggtg acaagctctc cttgcggcgt c
//
LOCUS       DAOQOC010000030         5884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5353_length_5884_cov_3.222851, whole genome shotgun sequence.
ACCESSION   DAOQOC010000030 DAOQOC010000000
VERSION     DAOQOC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5353_length_5884_cov_3.222851"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PL106_00845"
     CDS             complement(<1..933)
                     /locus_tag="PL106_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HNA31640.1"
                     /translation="MRHLYPIFFVLLLIRPVATWAQCTTTNATSCQCPPGQGTDCDLL
                     PDMTTSWYAALNYLSGPNEESGRIYVTSSTPNIGYGPLEVRGVDLNGYRRFVCGVDTF
                     EVYDPSASQQFACPNGGTAKQLTTQRIFHKNGPTMTSTEHVMPQGMTYHPTHGHTHYD
                     QWGIFSLRMQETGITDPRQWPIVGQGYKLGYCLMDYHNCNASAANHHCKDNNTVYNAG
                     TTLYGPDFPNLGLGGAYGCSMTRQGISSGYTDVYSEYLDGMWIDLPSGTCNGDYWIVL
                     ESDPLNVVVEANETNNWTAVPYTLTQQPNTAPVAE"
     gene            1541..2248
                     /locus_tag="PL106_00850"
     CDS             1541..2248
                     /locus_tag="PL106_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNA31641.1"
                     /translation="MSEISVRSEHATRLSELIERYCTPEWKQLMRERNVQLTFRKGQH
                     VFQQGQVAEHMFMIESGGVKVSATYEKSRDRIVRIAGPGEVVGYRALGDHMVYTATVT
                     ALMDTVVNSIPLSLFTNVLKANNAFCYHFLLFFANELRALDQQLRDHLEMSVLQRVAK
                     ALKLNLETFGTDAKDPKRLSFTLSRTDIARLADTSYESVIRSLTELQRAKVIALAGKQ
                     IRILKRREMERMAAPFR"
     gene            complement(2321..2611)
                     /locus_tag="PL106_00855"
     CDS             complement(2321..2611)
                     /locus_tag="PL106_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNA31642.1"
                     /translation="MSNGPSWLNIQGTVLHIVLHVLPGAVRSEVVGTHGDALKVRLAA
                     PPVDGRANEELIRFLAEQFGVPKRQVEILRGTTGRRKHVRITSPACRPDTGW"
     gene            2798..3358
                     /locus_tag="PL106_00860"
     CDS             2798..3358
                     /locus_tag="PL106_00860"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNA31643.1"
                     /translation="MPLTTFLLLFRGVGGPVQLPVAPLRAELTKAGFKNATTYINSGN
                     ALVRSTLSREETLTKVAALCLKKFNYDRPIFAPTAAEWDAVIKRDPFPQKAEGKHVHA
                     VLLAENPKAEAVKKLQALAVDGEALTLLRAPIGAYHVCYLHTPHGFGTSKLAEKFDKG
                     IGVVNTARNWNTVLKMQELARGLEVL"
     gene            3509..5254
                     /locus_tag="PL106_00865"
     CDS             3509..5254
                     /locus_tag="PL106_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNA31644.1"
                     /translation="MAHHPKKLGPLPATAIAGNDITSSCLYVSSLAIVHAGVWAVVGL
                     LLVAGVLFLFRRIYGEVVGALPLNGGAYNVLLNSTNKYNASIAACLTILSYMATAVLS
                     GTTAMHYLHALVPSWPILLSTVGVLLLFMLLSIAGITESASVATVIFIAHLSIMLLLI
                     LGGGWYVITHGFSVFSMNWHLPAPGGYSIGALIFLGFSTAMLGVTGFESSANYVEEQE
                     HGVFPKTLRNMWVAVSVLNPLIALIGLMVLPMATIAADSEAMLSVMGLEIGGDWLAMV
                     ISIDAVVVLCGAVLTSYVGVTGLIKRMTLDRILPQFLLKENKRGSSPRIIILFFLLCL
                     SVLLITNGKVEALAGVYTISFLTVMAFFALGNFMLKIKRARLPRPETASTAVVSVALF
                     GVLAALYGNIRMDPEYLVVFLQYFVPFMLVIALLLNRKAILEYLMIWVGGFVESMPGL
                     AVFGRLTISRTIRRLNKQEFVYFTKGDDLSIINHVMAYVVENEITRKLRIVTVLKDGE
                     HVKDEFLREVQVLDKAYDTIDVEFIQLSGTFGPELVHRLSAEWNIPTNFMFIGSPGDR
                     FPYRIAELGGVRLVI"
     gene            complement(5287..>5884)
                     /locus_tag="PL106_00870"
     CDS             complement(5287..>5884)
                     /locus_tag="PL106_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA31645.1"
                     /translation="TIAIPQGEMVAIMGPSGSGKSTFMNLLGCLDSPSGGSYRLDGQD
                     VSQLDSDALASIRNEKIGFVFQFHYLLPEFSVLHNVMLPGLKLGMKSSGEVEHRAMEL
                     LHELGIGDQALKKANQVSGGQKQRVAIARALINDPLIIMGDEPTGNLDSQNTGIVFDI
                     FRRIANEHKRSLLIVTHDPDFAKRTDRIIEMDDGRIVR"
BASE COUNT         1095 a         1737 c         1832 g         1220 t
ORIGIN      
        1 ctcggcgacc ggggccgtgt tcggttgctg cgtgagcgta tagggcacgg ctgtccagtt
       61 gttggtctca ttggcctcca cgaccacgtt cagcggatcg gattccagaa cgatccagta
      121 atcaccgttg catgttccgg agggcaggtc gatccacatg ccgtccaagt attcgctgta
      181 cacatcggtg taccctgagg agatgccctg gcgggtcatg ctgcagccat aggcccctcc
      241 caaccccagg ttcgggaaat cgggcccgta caacgtggtg cctgcgttgt acaccgtgtt
      301 attgtccttg caatgatggt tggccgcgct tgcgttgcag ttgtggtagt ccatcaggca
      361 gtatcccagc ttgtatcctt gtcccacgat cggccattga cgcggatcgg tgatcccggt
      421 ctcctgcatc cgcaaactga agatacccca ctgatcgtag tgcgtgtgcc cgtgcgtggg
      481 gtggtaggtc atgccttggg gcattacgtg ctccgtggag gtcatcgtcg gcccgttctt
      541 atggaaaatg cgctgggtgg tgagttgctt ggccgttccc ccgttggggc atgcgaactg
      601 ttgcgaggct gatggatcat agacctcgaa ggtgtcgacc ccgcacacga agcggcgata
      661 accgttgagg tcaacaccgc gcacttccaa gggcccgtag ccgatgttgg gggtggaact
      721 cgtgacatag atcctgcccg attcctcgtt gggtccgctc aggtagttga gcgccgcgta
      781 ccacgatgtg gtcatgtcgg gcagcaggtc gcagtcggtg ccctgcccgg gcgggcattg
      841 gcacgatgtg gcattggtgg tggtgcattg cgcccacgtt gcgaccgggc ggatcagtag
      901 caggacgaag aagatcgggt agaggtggcg catgggactc gttggattga tgcgaatttg
      961 aacgagatgg gcaccggtaa tctatgactt gtgtcatgat atggggttcc cgatccgacc
     1021 ggcctgtgtt ggttcccggc acaagagccc gtgcgcggtc ttgggcagga gtgccgctcc
     1081 ggctcattga tccaccggcc ggaccgagaa cctgccgcgt acacccgcta tcgcggacgg
     1141 cctggtgtgt tcatcaggct tatgcatgat cagtctcctt gtacttctgg atgcatgttg
     1201 cacatcatgt tttactgaca tagcggcacg aacctccacc cccggacggc acccccatga
     1261 caccacatcc ccccacgcct ggcccgaccg ttcagggtgc cctgctacgg gaacagcccg
     1321 acccccgcgg agaatacctc aacgtcctca acaaggacga tcacaggcaa gcgcttgtcg
     1381 ggaaggcgat caccacacgg tcccaacgac ggagctccgg caaagaccag gatggaggtc
     1441 acctctccgg aacaacatca cactcaccgc acctccggtc ggcgataccg cattgacggg
     1501 gccattcttg atggcgccgt ggaccggata gctttgcggc atgtcggaaa tatcggtgcg
     1561 gagcgagcac gccacccgct tgagcgagct gatcgagcgg tattgcacgc cggaatggaa
     1621 gcagttgatg cgcgagcgga acgtgcaact cacgttccgg aaaggacagc atgtgttcca
     1681 gcaagggcag gtggcggagc acatgttcat gatcgagagc ggtggtgtca aggtcagtgc
     1741 gacctacgaa aagagccggg accggatcgt gcggatcgct ggcccagggg aggtggtggg
     1801 ctacagggct ttgggggacc acatggttta cacggccacc gttaccgccc tcatggatac
     1861 cgtggtcaac agcataccct tgagcctgtt caccaatgta ttgaaggcca acaacgcctt
     1921 ctgctaccac ttccttctct tcttcgcgaa tgaattgcgg gccttggacc agcaactgcg
     1981 cgatcatctg gaaatgtccg tgctgcaacg ggtggcgaag gcactgaagc tgaacctgga
     2041 gacctttgga acggatgcga aggatccgaa gcggctcagc ttcaccctgt cgcgcaccga
     2101 tatcgcgcgg ttagcggata cttcctatga atcggtgatc cgctcattga ccgagttgca
     2161 gcgagccaag gtcatcgcgc tggcaggcaa acagatcagg atcctgaaac gaagggagat
     2221 ggagcgcatg gcagcacctt tccgatgatg gggatccgtt gtggcgcatg ggtacattcc
     2281 gttgttgcct gtgccaggag ccggcgggcc tccgctacgg tcaccacccg gtatccgggc
     2341 ggcaggccgg ggaggtgatc cgcacgtgtt tcctgcgacc ggtcgtacca cggaggatct
     2401 ccacctgccg cttcggaaca ccgaactgct cggccaggaa gcggatcagc tcctcattgg
     2461 cccggccatc cacgggtggt gcggcgaggc ggaccttcaa cgcgtcgccg tgcgtgccga
     2521 ccacttcgga gcgcaccgcc ccggggagca cgtgcagtac aatgtgcagc acagtccctt
     2581 ggatattcag ccaggatggt ccgttcgaca tgttcaggcc gaagatcgca atggtggctg
     2641 gatcgggcgc tccctcctgc atatcgcgac aacctgatcg ggtgcgttcg gatcgtggtg
     2701 tccgacgtca acacatccaa ggtcgcctct tcgaccgttc ggccatggag tcacggcagg
     2761 ccatgaacct gttgtgtcat ctttgtacga cccagcgatg ccgctcacca catttcttct
     2821 cctcttccgc ggtgtaggcg ggcccgtgca actgccagtc gcgcccttgc gtgcggaact
     2881 caccaaggcc ggattcaaga atgccaccac gtacatcaac agcggaaacg ccttggtgcg
     2941 cagtacgctt tcacgcgaag aaacactcac caaggtggcc gcgttgtgcc tgaagaaatt
     3001 caactacgac cggcccatct tcgcgccgac cgcggcggaa tgggatgctg tgatcaagcg
     3061 ggatccgttc ccgcagaagg ccgagggcaa gcatgtacat gccgtgctgc tcgccgaaaa
     3121 tcccaaggcc gaggcggtga agaagctaca ggcattggcc gtggacggtg aagcactgac
     3181 cctgctgcgt gcgcccatcg gtgcgtacca cgtgtgttac ctgcacaccc cgcacggctt
     3241 cggaaccagc aagttggccg agaagttcga taagggcatc ggtgtggtga acacggcccg
     3301 gaattggaac acggtgctga agatgcagga actggcacga ggactggagg tcttgtgatc
     3361 caggaatagg acgttgtggc aatgatgggt tgacaagtgc cacgctacga tcatgatcac
     3421 ctggtgttcg acacaatcac cacctgaaac gaggtatgtt gtgtgcggca ccgggggtct
     3481 tttatttgtg gtctcctgaa ccacccccat ggctcaccat cccaagaaac tcggcccatt
     3541 gcccgccacg gccatcgcgg gcaacgacat caccagcagt tgcctgtacg tgagctcact
     3601 cgccattgtc cacgccgggg tgtgggcagt ggtggggctg ttgctggtgg ccggtgtatt
     3661 gttccttttc cgcaggatct acggcgaggt ggtgggcgca ctgcccttga acggtggcgc
     3721 ctacaacgtg ctgctgaaca gcaccaacaa gtacaacgcg agcatcgcgg cctgcctcac
     3781 catcctgagc tacatggcca ccgccgtgct cagcggcacc accgccatgc actacctgca
     3841 cgccctggtg ccctcgtggc ccattctgct cagcaccgtc ggcgtgctgt tgctgttcat
     3901 gttgttgagc atcgcgggca tcaccgagtc ggcgtcggtg gccaccgtga tcttcatcgc
     3961 acacctctcc atcatgctgc tgctgatcct gggcggtggc tggtatgtga tcacacatgg
     4021 cttctcggta ttcagcatga actggcacct gccggcacca ggggggtaca gcatcggtgc
     4081 gctgatcttc ctggggttca gcacggccat gctgggcgtc acgggtttcg agagttcggc
     4141 caactatgtg gaagagcagg agcacggggt gttcccgaag accttgcgca acatgtgggt
     4201 ggcggtgagc gtgctgaacc cgttgatcgc cttgatcggg ttgatggtgc tgcccatggc
     4261 caccatcgcg gcggacagtg aggccatgct ttcggtgatg ggcctggaga tcgggggcga
     4321 ctggctggcc atggtgatca gcatcgatgc ggtggtggtg ctctgcggcg cggtgctcac
     4381 cagttatgtg ggcgtcaccg gcctgatcaa acgcatgacg ctggaccgca tcctgccaca
     4441 gttcctgttg aaggagaaca agcgcggcag ctcaccacgc atcatcatcc tgttcttcct
     4501 gctgtgcctc tcggtgctcc tgatcaccaa cggcaaggtg gaggctctgg ccggggtgta
     4561 caccatctcc ttcctcacgg tgatggcctt cttcgcgctg ggcaacttca tgctgaagat
     4621 caagcgggcc cgcctgcccc ggcccgaaac ggcctccacc gccgtggttt ccgtggccct
     4681 gttcggggta ttggcagcgc tctatggcaa catcaggatg gatcccgaat acctggttgt
     4741 tttcctgcaa tacttcgtac cgttcatgct ggtgatcgca ttgctgctga accgcaaggc
     4801 gatcctggag tacctgatga tctgggtggg cggcttcgtg gagagcatgc ccggtctggc
     4861 agtgttcggt cgcctcacca tttcccggac catccggcgt ctgaacaaac aggagttcgt
     4921 ttacttcacc aagggcgatg acctctcgat catcaaccat gtgatggcgt acgtggtgga
     4981 gaatgagatc acccggaaac tgcggatcgt taccgtactg aaggacggcg aacacgtgaa
     5041 ggacgagttc ctgcgtgaag tgcaggtgct cgacaaggcc tacgacacga tcgatgtgga
     5101 gttcatccaa ctgagcggca ctttcggccc cgagctggta caccgcctga gcgcggagtg
     5161 gaacatcccc accaacttca tgttcatcgg ttcgccgggc gaccggttcc cgtaccggat
     5221 cgccgagttg ggcggggtgc ggctggtgat ctgatcggga acggggccct acggaccatc
     5281 actcgttcat cgcacgatcc gcccgtcgtc catctcgatg atgcggtcgg tgcgcttggc
     5341 gaaatcgggg tcgtgggtga cgatgagcag gctgcgcttg tgctcgttgg cgatgcgtcg
     5401 gaaaatgtcg aagacgatcc cggtattctg actgtccagg ttgccggtgg gctcgtcgcc
     5461 catgatgatc agcgggtcgt tgatcagcgc ccgggcgatg gccacgcgct gtttctgccc
     5521 accgctcact tggttggcct ttttcaatgc ctggtcgccg atgcccagct catgcaacaa
     5581 ttccatggcc cggtgctcca cctccccgct gcttttcatg cccagtttca gcccgggcag
     5641 catcacgttg tgcagcacgg agaattccgg cagcaggtag tggaattgga agacgaagcc
     5701 gatcttctcg ttgcggatgc tggcgagcgc gtccgaatcg agctgcgaga cgtcttggcc
     5761 gtcgagccgg tacgagccgc cgcttggcga atcgaggcag ccgagcaggt tcatgaaggt
     5821 cgacttgccc gagcccgacg gccccatgat cgcgaccatc tcgccctgcg ggatcgcgat
     5881 cgtg
//
LOCUS       DAOQOC010000031         5804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5478_length_5804_cov_3.203340, whole genome shotgun sequence.
ACCESSION   DAOQOC010000031 DAOQOC010000000
VERSION     DAOQOC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_5804_cov_3.203340"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PL106_00875"
     CDS             complement(<1..553)
                     /locus_tag="PL106_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNA31646.1"
                     /translation="MDAKFSPRVREVITYSREEALRLGHNHIGIEHILLGLIREGDGN
                     AIRILRQLDVDLDDLRRTIESGMEPASAIKPPDKDKIALVKQAEKMLKITFLEAKLFK
                     SPFIETEHVLLSILKDMDNLATRTLNRFNVDYEQVKNELDAIMAGNQPNRPKPRPKAQ
                     GPSAEDDDDDSGSFAGGGGGPQKK"
     gene            973..3567
                     /gene="gyrA"
                     /locus_tag="PL106_00880"
     CDS             973..3567
                     /gene="gyrA"
                     /locus_tag="PL106_00880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNA31647.1"
                     /translation="MAGGEKIIPINIENEMRTAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMQELGVGSTRPYKKSARIVGEVLGKYHPHGDGSVYDAMVRMAQDWSLRYPLV
                     DGQGNFGSIDPDPPAAMRYTEARLRRIAEDVLADLDKETVDMRPNFDDTLEEPTVMPT
                     RIPALLVNGASGIAVGMATNMPPHNLTEVVDATIAYIGNKDIDTAGLMQHITGPDFPT
                     GGTILGTSGIKEAYETGRGRIVLRSTARIEEDPKTGRETIIISDIPYFVGKNALFKKS
                     CDLINEKKIEGVSDARDESDRNGIRIVYEVKRDAMASVVLNQLYKYTDFQTSFSVNNI
                     ALVKGRPVLLDLKSMIHHFVDHRMDVVIRRAKFELRKAEERAHILEGLLIALDHLDEV
                     IKLIRASATPDEARDGLMSNFGLSEIQAKAILDMRLQRLTGLERDKIRQEHAEIMELI
                     GRLKALLADEGLRYELITKELLEVKEKYGDKRRTAIITSGGDMSMEDLIADDAVVVTI
                     SHLGYIKRTALSEFRTQGRGGVGSRGSTTRDEDFLEHLFVATNHNYLLVFTQSGKVFW
                     MRVFDIPEGNKQSKGRAIQNLVQLEGGDKVVAYLNVKDITDKAYVDNHFVVLCTKQGI
                     IKKTTLEAYSRPRANGIIAVGIREGDELLEAKLTNGNNHIILASLDGRAVHFEESDVR
                     PMGRSASGVRGMNLEGGSKGNEVIGMIAMDKDDIAKRQVLVVSENGYGKRSAMVDENG
                     EYEYRMVNRGGKGVKTLNVTEKTGQLVAIKSVTENDHLMIITRNGITIRIGLEEMRVL
                     GRATQGVRLIELRDKDSIAAVAKVDSELHEDEDATPTEGPVEGSPDPDQNGDNPDGTE
                     LADGDNQE"
     gene            3586..4761
                     /locus_tag="PL106_00885"
     CDS             3586..4761
                     /locus_tag="PL106_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31648.1"
                     /translation="MRKTLLTLATWATLAATAQNSNVVSAYMALEDNKLDEAAGLIEP
                     TVTNENTMVKEKTWRYRGMIYQRIAHSDDASLKAKYPDALEKAIASYTKANELDVKGV
                     EKKANDQALRGLQVLALNSGNDAFTAKDYDKAVALYAQSEAVAKSFGEVDSNAVFNSA
                     LAFESKGDLGEALKRYQQCISFGYDKPEIYRYTASLQKRQNDLNGAIATTQVGRKKHP
                     DDKELMLDEVAFLQEAGRVNEVETSVAEALQKDPGNCVLHSLQASIFEAKADPKSGAK
                     PAEADMYKWYDAAEASYKKSIECDPKFFDGYFNIGVLYNNRAAYEYDKANNEKDNTKY
                     EKLKALADATFMKAVPYFEQAHQLKPDDRPTIQQLKTLYAKAQNKEKFEEMKKLLGE"
     gene            4852..5082
                     /locus_tag="PL106_00890"
     CDS             4852..5082
                     /locus_tag="PL106_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNA31649.1"
                     /translation="MELSDRITIEPGKMGGRPCIRGLRITVGTIMGLLAAKRTREEIL
                     ALYPYLEDEDIKAALHYATWLASDQELPIKRA"
     gene            5079..5444
                     /locus_tag="PL106_00895"
     CDS             5079..5444
                     /locus_tag="PL106_00895"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNA31650.1"
                     /translation="MKVVVDMCQSPLIADGLRTAGHDATHWPIIGPSNALDEEIARWA
                     VEHDAVVPTHDLDFGDLLAASRAKGPSVIIVREEDTMPERVPGPVLQVLAQFNADLAT
                     GSLISMTSQSARVRKLPMH"
     gene            5508..>5804
                     /locus_tag="PL106_00900"
     CDS             5508..>5804
                     /locus_tag="PL106_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31651.1"
                     /translation="MSQTTLSEKRVHMADLHSDHQLWLNTLRFCKDEIGIFDKRMEDI
                     ARRNTGREVMAELEHFQNRYIREREVIDELRHDIKQHENHLEKFAMDHPVASDHV"
BASE COUNT         1293 a         1752 c         1797 g          962 t
ORIGIN      
        1 gtttcttctg cgggccacca ccgccaccag cgaagctccc actgtcgtcg tcatcgtcct
       61 cggcactggg gccttgggcc ttgggtcggg gtttggggcg gttgggctgg ttgcccgcca
      121 tgatcgcgtc cagttcgttc ttcacttgct cgtagtccac gttgaatcgg ttcagggtgc
      181 gggtggccag gttgtccatg tccttcagga tgctcagcag cacgtgctcg gtctcgatga
      241 agggactctt gaacaacttg gcttccagaa acgtgatctt cagcatcttc tcggcctgct
      301 tcaccagggc gatcttgtcc ttgtcgggcg gcttgatcgc actggccggc tccatgccgc
      361 tctcaatcgt gcgccgcagg tcatccaggt ccacatccaa ttgccgcagg atacgtatcg
      421 cattgccgtc cccttcgcgg atcaagccca acaggatgtg ctcgatcccg atgtggttgt
      481 gccccagccg cagggcctcc tcgcgactgt aggtgatcac ctcccgtacc cgggggctga
      541 atttggcgtc catgttcatc ttctcggtga tttaggccat agcgtaccct cgacacccct
      601 tacgggcacc acaatgataa gcggtagacc ggtgggccaa gctatgctga tgggttccgg
      661 cggtgccggg gcatcgaacg gccttttttc cacatcggcc gtggtggttt gtggataagt
      721 cggggtcggg gcgcatgggc gatcggggta ctttcgggct ccctttcaaa aaggggtatg
      781 caaggacagt gcgcggacgg atgcgggtga cggaatggcc ggaaaagcga caaggcgcaa
      841 gccccgagct ggatcgccca cgtctccacc tgcaagctgc aaggctcaag gaaatgttcc
      901 ccggtcgtga tgtgtactgt tcgatgcttg acgcttgaaa acgtgtgacc tgaagctgtt
      961 catcacttga ccatggctgg aggagagaag atcatcccga tcaacatcga gaacgagatg
     1021 cgcaccgcct acatcgatta ttcgatgtcg gtgatcgtga gccgggccct gcccgatgtg
     1081 cgcgacggac tgaagcccgt gcatcggcgc gtactcttcg gcatgcagga actgggcgtg
     1141 ggcagcaccc gtccgtacaa gaagagcgct cgtatcgtgg gcgaagtgct cggcaaatac
     1201 cacccgcacg gcgacggcag cgtgtacgac gccatggtgc gcatggccca ggattggagc
     1261 ctgcgctatc ccttggtcga tggccagggc aacttcggta gcatcgatcc cgatccgccg
     1321 gcggccatgc gttataccga agcgcgcctg cgccgcatcg cggaggacgt gctggccgac
     1381 ctggacaagg agaccgtgga tatgcggccc aacttcgacg acacgttgga ggagcctacc
     1441 gtgatgccca cccgtatccc ggccctgctg gtgaacggcg cttcgggcat cgccgtgggc
     1501 atggccacca acatgccgcc acacaacctc accgaagtgg tggacgccac catcgcgtac
     1561 atcggcaaca aggacattga taccgcgggc ctgatgcaac acatcacggg ccccgacttc
     1621 cccacgggag gcaccatcct gggcacctcg ggcatcaagg aagcgtacga gaccgggcgc
     1681 ggccgtatcg tgctgcgttc gaccgcccgc atcgaggagg acccgaaaac cggccgcgag
     1741 accatcatca tcagtgacat cccgtacttc gtgggcaaga acgcgctctt caagaagagt
     1801 tgcgacctca tcaacgagaa gaagatcgag ggcgtgagcg acgcgcgcga cgagagcgac
     1861 cgcaacggga tccgcatcgt gtacgaggtg aagcgcgacg ccatggccag cgtggtgctc
     1921 aaccagctgt acaagtacac cgacttccag acctccttca gcgtgaacaa catcgcgctg
     1981 gtgaaggggc gccccgtgct gctcgatctc aagagcatga tccatcattt cgtggatcac
     2041 cgcatggacg tggtcatccg gcgtgccaag ttcgagttgc gcaaggccga ggaacgggcc
     2101 cacatcctgg agggcctgct gatcgccctg gaccacttgg acgaggtgat caagctgatc
     2161 cgcgccagcg ccacccccga tgaggcccgc gatggcctga tgagcaactt cggcctgagc
     2221 gagatccagg ccaaggccat cctggacatg cgcctgcagc ggctcacggg cctggagcgc
     2281 gacaagatcc gccaggagca cgccgagatc atggaactca tcggccgctt gaaggccctg
     2341 ctggccgacg agggactgcg ctacgagctg atcaccaagg agctccttga agtgaaggag
     2401 aaatacggcg acaagcgccg tacggccatc atcaccagcg ggggcgacat gagcatggag
     2461 gacctcatcg ccgacgatgc ggtggtggtc accatcagcc acctgggcta catcaaacgc
     2521 accgccctga gcgagttccg tacgcagggc aggggaggcg tgggcagccg tgggtccacc
     2581 acacgcgacg aggacttcct ggagcacctc ttcgtggcca ccaatcacaa ctacctgctc
     2641 gtcttcaccc agagcggcaa ggtcttctgg atgcgtgtgt tcgatatccc cgaagggaac
     2701 aaacaaagca agggccgcgc gatccagaac ctggtgcaac tggaaggcgg cgataaagtg
     2761 gtggcctacc tgaacgtgaa ggacatcacg gacaaggcat acgtggacaa tcacttcgtg
     2821 gtgctttgta ccaaacaggg catcatcaag aagaccacgc tcgaagcgta cagccgtccg
     2881 cgcgccaatg gcatcatcgc cgtgggtatc cgcgagggcg acgaactgct cgaggccaag
     2941 ctcaccaatg ggaacaacca catcatcctg gccagcctcg atgggcgggc ggtccacttc
     3001 gaagagagcg acgtgcggcc catggggcgc agtgccagtg gcgtgcgtgg catgaacctg
     3061 gagggtggca gcaagggcaa cgaggttatc ggcatgatcg ccatggacaa ggatgatatc
     3121 gccaagcgcc aggtcctggt ggtgagtgag aatggctacg gcaagcgctc ggccatggtg
     3181 gacgagaacg gcgagtacga gtaccgcatg gtgaaccggg gcggcaaggg tgtgaaaacc
     3241 ctgaacgtta ccgagaagac cggccaactc gtggccatca agtccgtgac cgagaacgac
     3301 cacctgatga tcatcacccg caatggcatc accatccgga tcggtctgga ggagatgcgt
     3361 gtgctgggcc gtgccacgca gggcgtgcgg ttgatcgagc tgcgcgacaa ggacagcatt
     3421 gccgccgtgg ccaaggtgga cagtgaattg cacgaggacg aggatgcaac gcccaccgaa
     3481 gggccggtgg agggaagccc ggaccccgac cagaacgggg ataaccccga tggcaccgaa
     3541 cttgctgacg gcgacaacca agaataggac acgaaccgaa caccgatgag gaagaccctg
     3601 ctcaccctgg ccacttgggc cacgctggcc gccacggcac agaacagcaa cgtggtcagc
     3661 gcgtacatgg cgctggagga caacaaactg gacgaggccg ccggtctgat cgagcccacg
     3721 gtcaccaacg agaacaccat ggtcaaggag aagacctggc gctatcgggg catgatctac
     3781 cagcgcattg cgcacagcga cgatgcctcg ctgaaggcca agtatcccga cgctttggaa
     3841 aaggccatcg ccagctacac caaggccaac gagctggacg tgaagggtgt ggagaagaag
     3901 gccaacgacc aggccctgcg gggattgcag gtgctcgcac tcaacagcgg gaacgatgca
     3961 ttcaccgcca aggactacga caaggccgtg gccctgtacg cgcagagtga ggcggtggcc
     4021 aagagcttcg gcgaggtgga cagcaacgcg gtgttcaaca gcgcgctggc cttcgagagc
     4081 aagggcgacc tgggcgaggc gctcaagcgc taccaacagt gcatcagctt tggctacgac
     4141 aagcccgaga tctaccgcta caccgccagc ctgcaaaagc ggcagaacga cctgaatggc
     4201 gctattgcca ccacccaggt cggccgcaag aaacaccccg atgacaaaga actgatgctc
     4261 gatgaggtag ccttcctgca agaggccggg cgggtgaacg aggtggaaac cagcgtggcc
     4321 gaggccttgc agaaggatcc cggcaactgc gtgctccaca gcctccaggc cagcatcttc
     4381 gaggccaagg ccgatccaaa gagcggggcc aaacccgcgg aggccgacat gtacaagtgg
     4441 tacgatgccg ccgaggccag ctacaagaag agtatcgagt gcgatcccaa gttcttcgac
     4501 ggctatttca acatcggcgt actgtacaac aaccgcgcgg cctatgagta cgacaaggcc
     4561 aacaacgaga aggacaacac caagtacgag aaactgaagg ccctggccga tgccaccttc
     4621 atgaaggccg tgccctactt cgagcaggcc caccaactca aacccgacga ccggcctacg
     4681 atccagcagc tgaaaacgct gtatgcgaag gcccagaaca aggagaagtt tgaagagatg
     4741 aagaaactcc tcggcgagta agccataccc gccgtggtgt taaggggagg gccctggcgc
     4801 aagccggggt tctctgtttc acgacgttcc catccctaac tttgacggag catggaactc
     4861 agtgatcgca taaccattga accgggcaag atgggcggac gcccttgcat caggggactg
     4921 cgcatcacgg tgggcaccat catgggcctc ttggcggcca agcgcacccg tgaggagatc
     4981 ctggcgctct atccatacct cgaggatgag gatatcaagg ccgcgttgca ttatgccacg
     5041 tggctggcga gcgatcagga acttcccatc aagcgggcat gaaggtggtg gtggacatgt
     5101 gccagtcccc gttgatcgcc gatgggctca ggaccgctgg acatgatgct acccactggc
     5161 ccatcattgg gccaagcaac gcgctggatg aggagatcgc gcgttgggcg gtggaacatg
     5221 atgccgtggt gccgacccac gacctcgatt tcggggatct gctggccgcc tcacgtgcca
     5281 aggggcccag tgtgatcatt gtgcgcgaag aagacacaat gcccgaaagg gtgcccggcc
     5341 cggttcttca agtcctggcg cagttcaacg cggatctcgc aacgggcagt ctgatctcca
     5401 tgacctctca aagtgcccgc gtgcggaagc tgccaatgca ttgatatccc ccgatcaccg
     5461 cctgcccgaa aattcccgac cttcatccca tcaaaacctc tacacccatg agccagacga
     5521 ccctctccga gaagcgtgta cacatggccg acctgcacag cgatcaccag ctctggctga
     5581 acacgttgcg cttctgcaag gacgagatcg ggatcttcga taaacgcatg gaggacatcg
     5641 cccgccggaa caccggccgc gaggtgatgg ccgaactcga gcacttccag aaccggtaca
     5701 tccgcgagcg ggaggtgatc gatgagctgc gacacgatat caagcagcac gagaaccacc
     5761 tggagaagtt cgcgatggac catccggtgg cctccgatca cgtg
//
LOCUS       DAOQOC010000032         5788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5492_length_5788_cov_3.370487, whole genome shotgun sequence.
ACCESSION   DAOQOC010000032 DAOQOC010000000
VERSION     DAOQOC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_5788_cov_3.370487"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PL106_00905"
     CDS             complement(<1..494)
                     /locus_tag="PL106_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA31652.1"
                     /translation="MRITIVGCGNMGTAYARSFRKYGLVKSDDLLLVVRSDSGHEQLE
                     RLGRVTRTVDGAITSSDLVIVAVKPQDFGALSTGLADVLRADQVVLSIMAGITLERLS
                     VALRHRTVVRAMPNAPAQIGMGITAYTTATELSMRQTLMVEQLLNSTGRAVHLSNETL
                     LDPVT"
     gene            complement(549..1259)
                     /locus_tag="PL106_00910"
     CDS             complement(549..1259)
                     /locus_tag="PL106_00910"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNA31653.1"
                     /translation="MLIANELGSCPQYAADGPRSARLGRKGAGTAIDRIPAWAVVLLL
                     SQPAYSQGTDARHKQALADFCLEYMEVRYPGVDLNGDILYVSVQRQRMFHVRERTMLR
                     EYMISTSTQGPGTERNSYRTPTGLHHVSERIGEGVPMWGIMKERLFTGELADSTCTDD
                     RDVITSRILWLRGLESGVNTGGNVDSHDRWIYIHGTVNEGSIGTPSSRGCIRMRNTDI
                     ITLFNEIPTGTLVLILDN"
     gene            complement(1259..2962)
                     /locus_tag="PL106_00915"
     CDS             complement(1259..2962)
                     /locus_tag="PL106_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31654.1"
                     /translation="MRTAALTIALLACTVLLAQPRYVVAAGGDALPKHWRGVQKVKGP
                     LGAQQWAKDVRADLMAKGWLEAACDTIATSGDTALCRFHTGPRYRWARLGAGNLPREI
                     ASATRFRERFYADRPVSPRQMSRLFEGLLVHCETNGHPFASVGLDSLRPSDDGLIAAM
                     HLERGPFIRIDSIIVRGDARIGARYLHSHIGIRPGDPYNEPLVSALDKRLRELPFVAV
                     KQRAQVRFGADQTKLYLFLDNKKASSFNGILGVLPDPVTGKVKLTGDLDLRLRNALRR
                     GEAIDLNWRALADRTQDLKVRYAHPFLFNTPFGTDLNLRLFKKDSTFLEVNARAAIDY
                     LLARGAKVSVFVNDKSSQRLGRTPSSTVTGLGDVKLTSYGLGLHHERFDYRFNPREGL
                     SVYLDGSVGRKRSITGSAEDPGGPVTTRTIQYELNGEAIQHLPIGKRGTIRLAGRGGT
                     MLNDRLYRNEIYRIGGIKSLRGVNEAGIFCSSWAVGTVEYRFLFEENSNFFVFFDQGW
                     WEDRSGDALLTDTPIGFGAGTSFETKAGIFSLTYALGRQFNNPVELRGGKIHFGFTSL
                     F"
     gene            complement(2962..3657)
                     /gene="ung"
                     /locus_tag="PL106_00920"
     CDS             complement(2962..3657)
                     /gene="ung"
                     /locus_tag="PL106_00920"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNA31655.1"
                     /translation="MNTEIGTGAPPRPDIGEGWYERLAGEFASPWFADLKAFLIEERS
                     RYTVYPRGKDIFQAFKRTPFDSVRVVILGQDPYHGPGQAHGLCFSVPEGLPSPPSLGN
                     IFAELQRDLGIARPDHGNLGKWADQGVLLLNATLTVRADQAGSHQGRGWERFTDAAIS
                     ALARERKGLVFLLWGRYAQNKEPLIGQDHHVLKAPHPSPLSAHRGFIGCGHFGQANGL
                     LCEQGHPPIDWSL"
     gene            complement(4090..4497)
                     /locus_tag="PL106_00925"
     CDS             complement(4090..4497)
                     /locus_tag="PL106_00925"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNA31656.1"
                     /translation="MDTALITWNGDLTNTCVHVRSGTHIRTDAPVDNHGKGSTFSPTD
                     LLCVSLATCILTTMGISAQQKGIPFNKASARVVKHMASDPRRVARIEVHISADGTGID
                     AKDRLVLERIAHTCPVARSLHPDVVQESTIAWT"
     gene            4603..5367
                     /locus_tag="PL106_00930"
     CDS             4603..5367
                     /locus_tag="PL106_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31657.1"
                     /translation="MRTATLLATALLLFFSARGQSIYDDEGRTLYSREIYGGPMVHGD
                     GWGLQFWFAKYRTARHRHLLGFEGLGMKHPKEIKSYNPYYEDSRGYFYGKLNSLIILR
                     PGYGGKHQIAEKIRKSGVELNWLWQVGPSIALLKPVYLQIGRRLDDQTGPPYDNIVEE
                     RYDPSKHFANNIFGRASWFRGVNEMKVRAGGFARLALNFEYSGRNRGVKGLEVGATAD
                     LYPVELPIMAEVSGVKNKQVFLEFYLALQFGAKTIR"
     gene            5364..>5788
                     /locus_tag="PL106_00935"
     CDS             5364..>5788
                     /locus_tag="PL106_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNA31658.1"
                     /translation="MSDIPGEVVATTPHHRRRKPDWLRVKLPTGDNYRKVSAIVGEHK
                     LHTICQSGNCPNMGECWGAGTATFMILGNICTRSCGFCNVATGRPLDADPFEPARVAR
                     SVQLMNVKHCVITSVDRDDLKDGGSDIWARTIRAVRRMSP"
BASE COUNT         1046 a         1791 c         1833 g         1118 t
ORIGIN      
        1 gtcacggggt cgagcagcgt ctcgttggaa aggtgtaccg cacgcccggt gctgttcagc
       61 agctgttcca ccatcaacgt ctggcgcata ctcagttcgg tggctgtggt gtaggccgtg
      121 atgcccatgc cgatctgcgc cggggcgttg ggcatcgcgc gaaccaccgt ccgatggcgc
      181 agcgcgacgg aaagccgctc cagggtgatg ccggccatga tggagagcac gacctggtcg
      241 gcgcgcaaga cgtccgccag tccagttgag agagcgccga aatcctgtgg tttaacggcc
      301 acgatgacca gatcgctgga cgtgatcgcc ccatccaccg ttcgcgttac ccggccgagc
      361 cgttcaagct gctcgtgacc gctgtcgctt cgcaccacga gcaggaggtc gtcggacttt
      421 accagcccgt atttcctgaa actgcgtgca taggccgtgc ccatgttgcc gcaacccaca
      481 atggtgatcc tcatgttccc tgcgtcggtc ctgttgaagg atggtgctgg cgaatgtagc
      541 cgcgttggtc aattgtccag gatcagcacc agtgttcccg taggtatctc gttgaaaagg
      601 gtgatgatgt cggtgttgcg catacggatg caaccccgac tgctgggggt tccgatcgat
      661 ccctcattga cggttccgtg gatgtagatc catcggtcat ggctgtccac attgccgccc
      721 gtgttcacac cgctctccaa gccgcggagc cacagtattc gtgatgtgat cacgtcgcgg
      781 tcatcggtac atgtgctgtc ggcaagttca ccggtgaaca gccgttcctt catgatgccc
      841 cacatgggaa cgccttcgcc gatgcgctca ctcacgtgat gcagaccggt gggcgtgcgg
      901 tagctgttcc gctcggtccc gggtccttgc gtggaggtgc tgatcatgta ctccctcaac
      961 atggtgcgtt cccgcacgtg gaacatgcgc tggcgttgaa cactcacgta caggatgtcg
     1021 ccgttgaggt cgacccctgg atagcgcact tccatgtact ccagacagaa gtccgccagt
     1081 gcttgtttgt gccgggcgtc ggttccttgg gaatatgcgg gctgtgaaag gagcaggacc
     1141 accgcccatg cggggatccg atcgatcgcg gtgccggctc ctttacgccc gagccttgcc
     1201 gatcgcgggc catcggccgc gtattgcggg caggatccga gttcgttcgc gatcagcatc
     1261 agaacaggct ggtgaacccg aagtggatct tgccaccacg caattccacg gggttgttga
     1321 actgccgccc cagcgcgtag gtcaggctga agatgccggc cttggtctcg aaactggtgc
     1381 ccgcgccgaa gccgatgggc gtgtcggtga gcaacgcatc cccgctccgg tcctcccacc
     1441 agccttgatc gaaaaaaacg aagaagttgc tgttctcctc gaagagaaag cggtattcga
     1501 cggtgccgac ggcccaactg gagcagaaga taccggcctc gttcacgcca cgcagacttt
     1561 ttattccgcc gatacggtat atctcattgc ggtacaggcg gtcgttcagc atggttccgc
     1621 cccgccccgc cagccggatc gtaccacgct tcccgatggg caggtgctgg atcgcttcgc
     1681 cgttcagctc gtattggatc gttcgcgtgg ttaccggacc accgggatcc tccgcactgc
     1741 cggtgatgct ccgcttgcgg cccacggacc cgtccaggta cacgctgaga ccctcgcgcg
     1801 gattgaagcg gtagtcgaag cgctcgtgat gaagacccaa tccgtacgag gtgagcttca
     1861 cgtcgcccag accggtgacg gtggaggacg gcgtacgccc gagacgctgg ctgctcttgt
     1921 cgttcacgaa gacgctcacc ttggcgccgc gggccagcag gtaatcgatg gcggcgcgag
     1981 cgttcacttc cagaaaggtg ctgtccttct tgaagagcct gaggttgagg tcggtgccga
     2041 agggcgtgtt gaagaggaac ggatgggcgt agcgcacctt caggtcctgg gtgcggtcgg
     2101 ccagcgcgcg ccagttgaga tcgatggcct cgccacgtcg cagcgcattg cgcaggcgaa
     2161 ggtccaggtc gccggtgagc ttcactttgc ccgtgaccgg atcgggcagc acgccgagga
     2221 tgccgttgaa gctgctggcc ttcttgttat ccagaaagag gtacagtttt gtctggtccg
     2281 cgccgaaccg gacctgggcc cgctgcttca ccgcgacgaa cggcaactcc cgcaagcgct
     2341 tgtccagcgc actcaccagc ggctcgttgt acgggtcgcc gggccggatg ccgatatggg
     2401 aatgcaggta gcgtgccccg atgcgggcgt cgccgcgcac gatgatgctg tcgatgcgga
     2461 tgaacggtcc acgttccaga tgcatcgctg cgatcaaccc atcgtccgaa ggccgaaggc
     2521 tgtcgagccc taccgaagcg aaaggatgac cgttcgtttc gcagtgaacg agcaggccct
     2581 cgaacagtcg ggacatctgc ctgggcgaaa ccggccggtc tgcatagaat cgctcgcgga
     2641 aacgggtggc actggcgatc tcccgtggca ggttccccgc accgagccgt gcccagcgat
     2701 agcgcgggcc cgtgtggaac ctgcacagtg ccgtgtcgcc cgatgtcgcg atcgtgtcgc
     2761 aagccgcttc aagccacccc ttggccatga ggtcggcccg cacgtccttc gcccattgtt
     2821 gtgctcccag gggccctttc actttttgca caccacgcca gtgtttcggc aaggcatcgc
     2881 ctcctgccgc caccacgtaa cgtggttggg ccagcagcac ggtgcaggcc agcagggcga
     2941 tcgtgagtgc ggccgtgcgc atcaaaggct ccagtcgatc ggtggatgcc cctgctcgca
     3001 caacaacccg ttggcctgac cgaaatgacc gcatccgatg aagccacggt gcgctgacaa
     3061 aggagaggga tgaggcgcct tgagcacgtg gtgatcctgg ccgatgagcg gctccttgtt
     3121 ctgtgcgtag cggccccaga gcaaaaagac cagccccttg cgctcccggg ccagtgcgct
     3181 gatcgccgca tcggtgaaac gctcccagcc ccggccttgg tgcgagcctg cctggtcggc
     3241 ccgcacggtc agggtcgcgt tcagcagcag cacgccctgg tcggcccatt tgcccaggtt
     3301 gccgtgatcc gggcgtgcga tgccaaggtc gcgttggagc tctgcgaaaa tgttccctaa
     3361 ggagggtggt gatggcaggc cctcaggtac gctgaagcac aagccatgcg cctggccagg
     3421 accgtggtag ggatcctgcc ccaggatcac cacccgcacg gaatcgaacg gtgttcgctt
     3481 gaaggcctgg aagatgtcct tgccccgggg gtagaccgtg tatcgggatc gctcctcgat
     3541 caggaatgcc ttcaagtcgg cgaaccaagg cgatgcgaac tcaccggcca ggcgctcgta
     3601 ccacccctcc ccgatatcgg gtcgaggagg ggcccctgtg ccaatttccg tgttcatggg
     3661 cgctattttg ccggtcccga tcgttgaaac attttggaag gtagcgcggt atcaggggct
     3721 acatttgtcc gaaccgctcc ccgtccagta acggcccaac gatgaaaaag cagacgaaaa
     3781 tactggcact tctggcgatc tgtgccgtgc tcttcagcgc atgccgctcc acccatgcgt
     3841 gcccagcata cggaaaggca ccgaaaccgt cgcctgaaaa gaacgcctga gcgatccctt
     3901 cgtgatcttc ttgaagcccc gcgaccacgg ggcttcggcc tttccacgcg tcctgtacgt
     3961 gttcggcgcc atatcctgcg cgcacaagat gtgacggtca gggggtgggc caagctggtg
     4021 tttttcccgc gagcatccgg cggatcgcag catggccctg ctaagcgacc gcgggagccg
     4081 ccctccccgt catgtccacg cgatcgtgct ctcctgtacc acatccggat ggaggctgcg
     4141 ggccaccggg caggtatgcg cgatccgctc cagcacgagg cgatccttgg cgtcgatccc
     4201 ggtgccatcc gcactgatgt gaacctcgat gcgagccacc cggcgcggat cgctggccat
     4261 gtgcttgacc acgcgggcac tggccttgtt gaacgggatc cctttctgct gggcactgat
     4321 gcccatggtg gtcaggatgc aggtcgccag tgatacgcac agcaggtccg ttgggctgaa
     4381 ggtggagccc ttgccgtggt tgtccaccgg ggcgtccgtg cggatgtggg tgccgctgcg
     4441 cacgtggacg caggtgttcg tgaggtcgcc attccaggtg atcagagccg tatccatggg
     4501 caagggtttg acgagtgtta aaggtcgcca ggggcgaaag atgggcatcc gactgttggc
     4561 gggactacct ttgggcctct tgatccaagc cccatgtgga ccgtgcgcac cgcaaccctg
     4621 ctcgcaacgg ccctgctgct gttcttctca gcgcgggggc aaagcatcta cgacgatgag
     4681 gggcgcacgc tctactcgcg ggagatctac ggcggtccca tggtgcatgg ggatggctgg
     4741 ggcttgcagt tctggttcgc gaagtaccgc accgcgcgcc accggcacct tctcgggttc
     4801 gaggggctgg gcatgaagca ccccaaggag atcaagagct acaacccgta ttacgaggat
     4861 tcgcgcgggt atttttatgg aaagttgaac agcctgatca tcctgaggcc cgggtacggt
     4921 ggcaagcacc agatcgcgga aaagatccga aagagcgggg ttgaactcaa ttggctctgg
     4981 caggtgggcc cctccatcgc gctgctcaag ccggtgtacc tgcagatcgg tcggcggctc
     5041 gacgatcaga ccggaccacc atacgacaac atcgtggagg aacggtacga cccttcgaag
     5101 cacttcgcca acaatatttt cggcagggcg tcctggttca ggggcgtcaa cgagatgaag
     5161 gtgcgcgcgg gcggtttcgc gaggcttgcc ctcaatttcg agtattccgg tcgtaaccgt
     5221 ggcgtcaaag gcttggaagt cggcgccacc gcggacctgt accccgtgga actgcccatc
     5281 atggccgagg tgtccggggt gaagaacaag caggtcttcc tggaattcta cctggcgttg
     5341 cagttcggcg ccaagacgat ccgatgagcg atatccctgg ggaggtggtc gccaccacac
     5401 cgcatcaccg ccgccgtaaa cccgattggc tgcgcgtgaa attgcccacc ggcgataact
     5461 accggaaggt ctccgccatc gtcggcgagc acaagctcca caccatctgc cagagcggta
     5521 attgccccaa catgggggag tgctggggtg cgggcacggc caccttcatg atcctgggca
     5581 atatctgcac gcgcagttgc ggcttctgca acgtggctac cggacgccca ctggatgccg
     5641 atcccttcga gcccgcccgc gtggcccgca gcgtccaact catgaacgtg aagcactgcg
     5701 tgatcaccag cgtggaccgc gacgacctga aggacggagg ctcggatatc tgggcgcgga
     5761 ccattcgtgc cgtgcgccgc atgagccc
//
LOCUS       DAOQOC010000033         5785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5500_length_5785_cov_3.234555, whole genome shotgun sequence.
ACCESSION   DAOQOC010000033 DAOQOC010000000
VERSION     DAOQOC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5500_length_5785_cov_3.234555"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PL106_00940"
     CDS             <1..222
                     /locus_tag="PL106_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31659.1"
                     /translation="HAGIPDVVEHEVHGLLCTEGDTRDMAEHMLRLGSEPLLAGRLGA
                     AGRERVVREHQLGDSLRRLHAILENAARH"
     gene            232..1410
                     /locus_tag="PL106_00945"
     CDS             232..1410
                     /locus_tag="PL106_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA31660.1"
                     /translation="MRIAYLTHYAELYGANRSLLDLMLELRSRGEVEPFVLLPREGPI
                     TERLQAEKIPFQVIPWEPWMSDRRYMGRIHHRIMQHLGYERAARKRAMHNRSLLPAVH
                     RTIEEQRIGLVHVNSAAVGITALLLEQCTAPLVWHIRELPEHQYMLTIDAGRRRYGQA
                     LRKAARLIAISKAVQEDIRRYAGSGVHADLIYNGVLRSADQQRLASKAMGRWTATGPF
                     TFVLAGLIHASKGQEEAIRAFALLRQHVQHVRLVLAGDGKADRLHALIQQLGVGDGVV
                     LTGFVKDLEPLLLSSHALLMCSRNEAMGRVTVEGMSSGLPVIGHASGGTLELITEGVT
                     GLFYPGGAEALAERMLALVNDRSRARTLGEAAMRDAAERFNVERYATEVLEVYRSLQH
                     "
     gene            1465..2463
                     /locus_tag="PL106_00950"
     CDS             1465..2463
                     /locus_tag="PL106_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA31661.1"
                     /translation="MARSRVSLITPSYQQAAYLEECINSVHDQGEAVEHIVVDGGSTD
                     GSKGIIERNGARLAWWCSEKDKGQSDAINKGLSHATGGIFNWLNSDDALLPGALKRAC
                     DAFEADPELLVYGGTLRHLVPGGTRSSNALNDTRDADRLYIDPVINQPATFWRMDVVR
                     AIGGVDPALRYVMDLELWWQLLFRHGTAHLCFEPVELASFRLHETSKTSTAILGFLDE
                     IATLLAGLCERTGNNDLVQVLRTGHTMRAGLRGIPVSQEQRDRVRRMTLHFLFKWNAE
                     IHRENQFDMMRTFKDLRIGMDTLTPEQQQRWQAILPGLSRPWSLFRARRKLKHLLR"
     gene            2460..3467
                     /locus_tag="PL106_00955"
     CDS             2460..3467
                     /locus_tag="PL106_00955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNA31662.1"
                     /translation="MKASVLIPVYNKAPFVKEAVESVLSGTFRDIEVVCVDDGSTDTS
                     LQVLHTIHDPRLRIIALPGNRGPAAAANAGIDACIGEYIVRLDADDIAVADRVARQVT
                     FMDTHPDIGASGGQLELFGATNKTWAFPLDPDDCAAQLLFGVPVSQGASILRRSVLEE
                     HGLRYDPTWPRFGEDWLFWLRMARVTRFANIPDTVALYRRGEHNIGHGRDRVADYDLL
                     VRAALGSYKIPFNEAEVAAHLMGLYIFKDPPTRSSVRRLRAWHGRLLAINSERGLFPR
                     KAFAERVTRSWERLFFYLARYGSAAALEHMRLSGSWPGDRLAYLAKYRVNALLGRVTN
                     G"
     gene            3470..4720
                     /locus_tag="PL106_00960"
     CDS             3470..4720
                     /locus_tag="PL106_00960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA31663.1"
                     /translation="MGTLDPSSGTGRELWLFTIRFPFGNGESFIENELPILARGFERI
                     RLFPLMPDGPARPIPGNAEVTNILGADAYRPASLPSVLMDLRRWLHVVRTSKASVTDR
                     SVWKARRRELYSRLRQAMRRERILLDRFGDRYDPERVVLYSYWTSDWATVLGLWKLRD
                     PRVRFVSRMMGFDMFDHRAKGNWQMLQAFHVEQVDRIFTIARAGQKYMGERHPHAREK
                     FMLSPLATVDHGPAPWAPSPILRIASCANLIPLKRVGLLVEALARLTIPVKWTHFGDG
                     EERSRIEALVRDLPPIIEVELKGSRPNSEVIQWYKANPVDVFVHTSSTEGGAPVALQE
                     AASFGIPLLAADAGGVNEIVTPWTGVLLPHAVSVDELESYLYHFRNSTWYQQRAREQV
                     REFWRAHFEAEVVHGRLLEFLLRT"
     gene            4775..>5785
                     /locus_tag="PL106_00965"
     CDS             4775..>5785
                     /locus_tag="PL106_00965"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA31664.1"
                     /translation="MLFNSAEFCLLFFPLVTGLYFVLPKNWRWLLLLVASCWFYMAFV
                     PLFILILAFTIGVDYVAGIAIARSEGPRRKAYLVASIIANVGVLAVFKYWDFLNGNLT
                     LLFAGMGLEYHVPKLHEWTFFGFLPAGMVLPIGLSFHTFQSLSYTIEVYRKHQPAERR
                     FGTFALYVMFYPQLVAGPIERPGNLLNQLNAQDGQGIRSDFEYARVVLGLKQMLWGFF
                     KKLVIADRCAVLVDEVYGHTAHYGAASLTLATALFALQIYGDFSGYTDIALGAARVMG
                     FNLMVNFRTPYRSASISEFWGRWHISLSSWFRDYLYIPLGGNRVAKWRWYTNLFIVFL
                     VSG"
BASE COUNT         1052 a         1826 c         1866 g         1041 t
ORIGIN      
        1 catgccggta tacccgacgt ggtggaacac gaagtgcatg gtctcctgtg cacggaaggt
       61 gacacgcgcg acatggccga acacatgctg cgtttgggga gcgaaccgct actggccggg
      121 cgcttgggcg cggcagggcg tgagcgggtc gtacgcgaac accagctcgg cgacagcctg
      181 cgacgcctgc acgccatcct ggagaacgcc gccaggcact gagctgccgc catgcggatc
      241 gcctacctca cccactacgc ggagctgtac ggcgccaacc gcagcctgtt ggacctgatg
      301 ctcgaactgc gttcgcgtgg agaggtggaa cccttcgtgc tgctgccgcg tgaaggtccg
      361 atcaccgagc ggctacaggc agaaaagatc ccgttccagg tgatcccatg ggagccctgg
      421 atgagcgatc gccggtacat gggacgcatc catcaccgga tcatgcagca cctgggatac
      481 gagcgtgcgg cgcggaagcg ggccatgcac aaccgctctt tgctaccggc ggttcatcga
      541 acgatcgagg agcaacggat cgggttggtc catgtgaaca gcgcggccgt ggggatcact
      601 gcactgttgt tggagcagtg cacggcaccg ttggtgtggc acatccgcga gctaccggaa
      661 caccagtaca tgctcacgat cgatgcgggc aggcgtcggt atggccaggc acttcgcaag
      721 gccgcacggc tcatcgcgat ctccaaggcc gtgcaggagg acatccggcg atatgcggga
      781 agtggcgttc atgcggacct catctacaac ggtgtgctcc gcagcgccga tcagcaacgg
      841 cttgcctcga aagccatggg ccgatggacc gccactggcc cgttcacctt cgtgctcgcg
      901 gggctgatcc acgcctccaa aggacaggaa gaggcgatcc gcgccttcgc gctgcttcgc
      961 caacatgtgc agcacgtacg cctcgtgctc gccggcgacg gcaaggcgga tcgcctgcac
     1021 gcgctcatcc aacaactcgg tgtgggcgat ggtgtggtgc tcaccggttt tgtgaaggat
     1081 ctggagccgc tcctgctgag cagccatgcc cttttgatgt gcagccggaa cgaagccatg
     1141 ggtcgtgtaa cggtggaggg catgagcagt ggtcttccgg tgatcggtca tgccagcggc
     1201 ggcacactcg agctgatcac ggaaggagtc accgggctgt tctatcccgg cggtgcggaa
     1261 gcgctggccg aacgcatgct ggcgctggtc aacgaccggt cgcgtgcccg aacactcggc
     1321 gaagcggcca tgcgcgatgc agcagaacgc ttcaacgtgg aacgctacgc gactgaagta
     1381 ctggaggtct atcgctcgct ccagcactga tccgacgggc attccgaccc gggggacggt
     1441 ctattttcgc gaccccttca cgccatggcc cgctcccgcg tctctctgat cacccccagc
     1501 taccagcagg ccgcgtatct ggaagagtgc atcaattcgg tacacgacca gggcgaagcg
     1561 gtggagcaca tcgttgtgga tggcggcagc accgacggat cgaaggggat catcgagcgg
     1621 aacggtgcgc gactggcctg gtggtgttcg gaaaaggaca aggggcagag cgatgcgatc
     1681 aacaagggac tgtcgcatgc caccggtggc atcttcaatt ggttgaacag cgatgatgcg
     1741 ctcctgcccg gtgcgttgaa gcgagcatgc gatgcgttcg aagcggatcc ggaactgctc
     1801 gtctacggcg gtacgctgcg acacctcgtt ccgggcggaa cccggtcgag caatgcactc
     1861 aacgacacgc gggacgcgga caggctgtac atcgatccgg tgatcaatca gcccgcgaca
     1921 ttctggcgca tggacgtggt acgcgcgatc ggtggcgtgg acccggcgct ccgctacgtg
     1981 atggacctgg agctgtggtg gcagctgctc ttccgccatg gcacggcgca cctgtgcttc
     2041 gagccggtgg aactcgccag cttccggctt cacgagacca gcaagaccag caccgcgatc
     2101 ctcggtttcc tggacgagat cgccacattg ctggccgggc tgtgcgagcg cacgggcaac
     2161 aacgacctgg tccaggtgct gcgcacagga cacacgatgc gggccgggct tcgtggcatt
     2221 cccgtgagtc aggaacagcg cgaccgcgtg cggcgcatga ccctgcattt cctcttcaag
     2281 tggaacgcgg agatccaccg cgagaaccag ttcgacatga tgcgcacctt caaagacctg
     2341 cgcatcggca tggataccct caccccggaa cagcaacaac gttggcaggc catcctgccc
     2401 ggcctgtccc gcccgtggag cctcttccgg gcccgccgca agctcaagca cttgttgcga
     2461 tgaaggccag cgtgctgatc cccgtgtaca acaaggcgcc cttcgtgaag gaggccgtgg
     2521 agagcgtatt gagcgggacc ttccgtgaca tcgaggtggt gtgcgtggat gatgggagca
     2581 ccgatacgag tcttcaggta ttgcacacga tccatgaccc gcgcctgcgc atcatcgccc
     2641 tgcccgggaa tcgcggtccc gcagcggcgg ccaatgcggg catcgacgcg tgcatcggcg
     2701 agtacatcgt gcggctggat gcggacgaca tcgctgtggc tgaccgggtg gcccggcagg
     2761 tgaccttcat ggacacgcat cccgacatcg gtgcgagcgg cgggcagctg gagttgttcg
     2821 gcgccacgaa caagacctgg gccttcccgt tggaccccga tgattgcgcg gcccaactgc
     2881 tgttcggtgt gccggtatcg cagggtgcca gcatcctgcg tcgcagtgtg ctggaagagc
     2941 acggtctgcg ctacgacccc acgtggccgc gcttcggcga ggactggctc ttctggttac
     3001 gcatggcccg cgtcacccgc ttcgccaaca taccggacac ggtggcgctg taccgcaggg
     3061 gcgagcacaa catcgggcat ggccgggacc gcgtagccga ctacgacctc ctcgtgcgcg
     3121 cggcactcgg ttcctacaaa ataccattca acgaggcgga ggtcgccgcg cacctgatgg
     3181 gcttgtacat cttcaaggat ccacccacgc gctccagtgt gcgtcggctg cgtgcctggc
     3241 acggccggtt gctcgccatc aactccgagc gcgggctatt cccacgaaag gccttcgccg
     3301 agcgggtcac gcgctcgtgg gaacgactct tcttctacct ggccaggtat ggttccgccg
     3361 ccgcgttgga gcacatgcgc ctgagcggtt cctggcccgg cgaccgactg gcgtatctgg
     3421 ccaaataccg cgtgaacgca ctgctcgggc gggtgaccaa cggttgatca tggggaccct
     3481 ggatccatcc tctggtacgg gccgcgaact atggttgttc acgatccgat tcccgttcgg
     3541 caacggcgaa tccttcatcg agaacgaact gccgatcctg gcacgcgggt tcgagcgcat
     3601 ccgcctgttc ccgctgatgc cggacggtcc cgcacgcccg atccccggca acgcggaggt
     3661 cacgaacatc ctgggggccg acgcgtacag gccggccagc ctgcccagtg tgctgatgga
     3721 cctgcggcga tggcttcatg tcgtgcggac aagcaaagcc tcggtaacgg accgctcggt
     3781 gtggaaggcc cgccgacgcg aactctacag ccggcttcgc caggccatgc gccgcgaacg
     3841 gatcctgctc gaccgtttcg gggaccgata cgatccagag cgagtggtac tgtattcgta
     3901 ttggaccagc gactgggcca cggtactcgg cctgtggaag ttgcgcgacc cgcgcgtgcg
     3961 tttcgtcagc cggatgatgg gcttcgacat gttcgaccac cgggcgaaag ggaactggca
     4021 gatgctgcag gccttccatg tggaacaagt ggaccgcatc ttcaccatcg cacgggcggg
     4081 acagaagtac atgggcgaac gccacccgca cgcgagggaa aaattcatgc tgagcccgct
     4141 ggccaccgtg gaccacggcc cagccccatg ggcaccgtcc cccatcctgc gcatcgcgag
     4201 ctgtgccaac ctgataccgc tgaaacgcgt gggcctgctg gtcgaagcgc tggcgcggtt
     4261 gaccataccc gtaaagtgga cccacttcgg cgatggggag gaacggagca ggatcgaagc
     4321 actggtgcgg gacctgccac cgatcatcga agtggaactc aagggcagtc ggcccaatag
     4381 cgaggtgatc cagtggtaca aagccaaccc ggtggatgtg ttcgttcaca ccagcagtac
     4441 cgaaggcggc gcacccgtgg ccctgcaaga ggcggccagc ttcggcattc ccttgctggc
     4501 ggctgatgcg ggcggggtga acgagatcgt gaccccgtgg accggtgtat tgctgcccca
     4561 cgcggtttcg gtggacgaac tggagagcta cctgtaccat ttccgcaata gcacctggta
     4621 ccaacagcga gcacgcgaac aggtccggga gttctggcgg gcacacttcg aggcggaggt
     4681 ggtgcacggc cgcctgctgg agttcctgct ccgaacctga gccgaacaac ggagcagcat
     4741 cggggtgcga catttgcgcc cttcgcccca gcggatgctt ttcaacagcg ccgaattctg
     4801 cctgctcttc ttcccgctgg taacggggtt gtacttcgtg ctgcccaaga actggcgttg
     4861 gctcctgctg ctggtggcga gctgttggtt ctacatggcc ttcgtgccct tgttcattct
     4921 catcctggcc ttcaccatcg gggtagacta tgtggccggc atcgccatag ctcgcagcga
     4981 ggggccccgg cgcaaggcgt acctggtggc cagcatcatc gccaacgtgg gcgtgctggc
     5041 ggtcttcaaa tactgggact tcctcaacgg caacctcacc ttgctgttcg ccgggatggg
     5101 gctggaatac catgtgccca agctgcacga gtggaccttc ttcggctttc tcccagccgg
     5161 catggtgctg cctatcggcc tgtcgttcca cacctttcag agcttgagct acaccatcga
     5221 ggtctaccgc aagcaccagc cggccgagcg gcgcttcggc accttcgcgt tgtacgtgat
     5281 gttctatcct caactcgtgg cgggccccat cgagcggccg ggcaacctgc tgaaccagtt
     5341 gaacgcgcag gacggccaag gcatacgatc ggacttcgag tatgcgcgcg tggtgctcgg
     5401 gttgaaacag atgctctggg gcttcttcaa gaaactggtg atcgccgacc gttgcgccgt
     5461 gctggtggac gaggtgtacg gccacaccgc gcattatggt gccgcgagcc tcaccctggc
     5521 aacggcgctg ttcgcgctgc agatctacgg cgacttcagc ggctacaccg acatcgcgct
     5581 gggcgccgcc cgggtgatgg gcttcaacct gatggtcaac ttccgcacgc cgtaccggag
     5641 cgcgagtatc agcgagttct ggggccgctg gcacatcagc ctcagctcct ggttccgcga
     5701 ctacctctac atcccgctcg ggggcaaccg cgtggccaaa tggcgctggt acaccaacct
     5761 gttcatcgta ttcctggtga gcggc
//
LOCUS       DAOQOC010000034         5645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5718_length_5645_cov_3.535063, whole genome shotgun sequence.
ACCESSION   DAOQOC010000034 DAOQOC010000000
VERSION     DAOQOC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_5645_cov_3.535063"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PL106_00970"
     CDS             complement(<1..1347)
                     /locus_tag="PL106_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31665.1"
                     /translation="MGRGAIAEHPDEIVLDCDDGYAGLPQKVRLAFAWALENGYDLCF
                     KCDDDTYVCPERLMRHMPVRHHWCGRLRGPSGNWRHPYTSGFGYWLSATAMRHRVHDA
                     QPEDVAEDRVTGTIMAAAGIRCHNDHRYIVASSRRNVPSGYDEGPRRGNDIIAACEYG
                     PQDMLRVHHEYLHAISERPSAKLLRGTPFDEVDVLVKTFLRDGLLERCVASLREHLPG
                     ARIVVVDDGYDSRPKTRLYHELRRQGHAVQWMSFDSGFGAKSNEAKLHYERPFVLVFS
                     DDFQMTPESAKGVERMLSVLRHDPHIGSAGGRVDARAYEADIVEAVRPDGLKDVRLIP
                     PASELQQAPPIPSEPSEPTVYRLCHQTVNYNLVRTEALADAMWDEKWKIGGDHLYFYQ
                     QILAAGRVAVLLGHEDGQAQGHAARQDGDFADRMGFRNGPGDHRVAGFVVGGHLALI"
     gene            1466..1867
                     /locus_tag="PL106_00975"
     CDS             1466..1867
                     /locus_tag="PL106_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31666.1"
                     /translation="MGPNLRTPDPDRKHLSPKNVVLFDLTAPLKEISDALLRVEELPD
                     MATRNIAAAAIERLVDRALMLRARTLGRPFRPSIITGASMSVERMERLQQQAEERREA
                     KEERRRKNREEWAMLNRATGHASKWWLQRNE"
     gene            1871..4264
                     /locus_tag="PL106_00980"
     CDS             1871..4264
                     /locus_tag="PL106_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31667.1"
                     /translation="MEQYDDLLLALSDAGMPLSQEDRDRIGERAGAPPPDRAQDIDRP
                     GLIMDGRQHLWLTPQQARAARERSENPQPRAECYLDRIPQAWKDEWRDREISRDDWCP
                     MDAKTLARNDEFRAFHLSHIPRFDRIAPYEPFWLFIEQALRWLAEDTSLYDVRAEDRY
                     AFVQEEFRRMDENHLYGALKYGYIPDDSMPGGVRKYDASTPQALILYWCAVGLSGELF
                     KGRQSAVTSTFELDEAISILTTPSSSAVLVTDDVKYTGEKIFKQKFKSMISLMVDRNP
                     WMDADKWLWSASSVTATWTERDRKGASNAMSAERTVAASGDTQVINGTNVSRIKIDEA
                     QNVGPYSGIKREVRPAMVSFVDGVARIKRSLWAYGTGNAKNSGSEFTSEYKATMEAFR
                     KGRDTSTYVPGFFDWTCRPGMTEKDLIEEYDFFMNASEGDMASRSSEERLAMFYSAHP
                     NKPEDAYLTLHRHVVPVTVIAEAWELIERHCTKRGIPVLGHFDPVFNTSVPMAEGSWY
                     PHLVVGSKWVPHAPDSLNSPCRMFLPKKSAERWVYRWAQGTDPAVVGSGSSMFASAIF
                     DGAGAWRDRDGARTFNPTVACLLNDSKQNMEECFNQSILMGMYYANDGQGACPELVEA
                     NRGGEYTRWKSSSMFNLSRSLLYRTQMPPKYQGGGHLYGIDMKNNDRNGRKTELFFDI
                     VAFIRQYGPRADESGKKRLLNIWYYDYWTQLYNIEVDEQATGAIKFGTRSKNLYNDDI
                     VYAVQYANIAHTVYAGAHTPMMINTHAPEFRVVRKPVRDMNTLEVRYEEVREPVIYV"
     gene            4266..4868
                     /locus_tag="PL106_00985"
     CDS             4266..4868
                     /locus_tag="PL106_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31668.1"
                     /translation="MAIQDRYLDVLAELAKKGYLVHAPISAKGLRQDYPELNDVAEFK
                     PLSSAELLMVWWFASRCSPIIEMPEEKRILVAIDQSFRSPAMAEARKIEYGTLSFTND
                     NIKAAIKRMDAFDPGGRITAMADDLHLLRQCQTVIRKDISKADMEEITEWMKAVSTAR
                     KLRDEILQRIERGSLGVADETTVSASLKGAAREYMKNKTN"
     gene            4873..>5645
                     /locus_tag="PL106_00990"
     CDS             4873..>5645
                     /locus_tag="PL106_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31669.1"
                     /translation="MSTLNNIMWAWPSMSPVSRPPMDIPWEKKDEAYDDTVMRYFLSG
                     MPSVYRDSYLKEYAANLAMVQNEPLSFGSPQMIREFLGDENNPTGRLALRYAIGQAMH
                     TRLVGVAESISVHARAEGWTSNVAARKDAALYERLLKSQMAQAGSPLTEAMAQVGLSP
                     DEDGERDRHENLWTDPYESAISNLIAAMGERQKLTSTIRLAASHLSLSGVVATHHYNN
                     GNHIERELCEPAEVGWDPSATRENLSDGEFCYVAPLMDVS"
BASE COUNT         1126 a         1831 c         1762 g          926 t
ORIGIN      
        1 gatgagcgcg aggtgaccgc ctaccacgaa gccggccacg cgatgatcgc cagggccatt
       61 ccggaagccc atccggtccg caaagtcacc atcctgccgc gcggcatggc cctgggcttg
      121 accatcttcg tgcccgagga ggaccgctac acggcccgcc gcgaggatct gctggtagaa
      181 atacaggtga tccccgccga tcttccactt ctcatcccac atcgcgtccg ccagcgcttc
      241 ggtacgcacc aggttgtagt tcaccgtctg gtggcacagg cggtataccg tgggttcgga
      301 gggttctgat gggatgggcg gcgcttgttg caactcagaa gccggaggga tgagcctcac
      361 gtccttcagt ccgtcggggc gcaccgcctc cacgatatcg gcctcgtacg cacgggcgtc
      421 cacccgccct ccggcgctcc cgatgtgcgg gtcatgcctg agcaccgata gcatgcgctc
      481 cacccctttg gcgctttcgg gcgtcatctg gaagtcatcg ctgaacacca gtacgaaggg
      541 gcgttcgtag tgcagcttgg cctcgttgct cttggctccg aagccgctgt cgaagctcat
      601 ccactgcacg gcatgccctt gccgcctcag ttcgtggtag aggcgcgtct tggggcggga
      661 atcatacccg tcatcaacaa cgacgatgcg cgcccccggc aggtgctccc gcaggctcgc
      721 cacgcaccgc tccagcagcc catcccgcag gaaggtcttc accagcacgt ccacctcgtc
      781 gaacggtgtg ccgcgcagga gtttcgccga cggccgttcg ctgatggcgt ggaggtattc
      841 atggtggacc cgcagcatgt cctgtggccc gtactcgcac gcggcgatga tgtcgttgcc
      901 gcgccggggc ccctcatcgt atccggaggg gacgttgcgc cgggaagagg ccacgatgta
      961 gcggtggtcg ttatggcagc gtatgcccgc cgccgccatg atggttcctg taacgcggtc
     1021 ctcggcgaca tcttccggct gtgcgtcgtg tacacggtga cgcatggccg tcgcggacag
     1081 ccagtagccg aagccggagg tgtagggatg acgccagttc cccgaggggc cgcgcagccg
     1141 cccgcaccaa tggtggcgaa cgggcatgtg gcgcatcagg cgctcggggc atacgtacgt
     1201 atcgtcgtcg cacttgaagc acagatcata gccgttctcc aaggcccacg cgaaggcaag
     1261 gcgcaccttc tgaggcagac cggcgtagcc gtcatcgcag tccagcacga tctcatcggg
     1321 atgctcggcg atggcgccgc gccccatgaa cacgcgcaca tccgccccat ctaccaatgg
     1381 gatccacgtt tcccgctggg actgcacctt ggcccggtaa ggggctgcgc cacaagacac
     1441 tatcgcaatt gctacttttg gcgacatggg cccaaaccta cggacacccg atccggaccg
     1501 gaagcatctc tcccccaaga atgtggtcct cttcgacctt acggcgcctc tcaaggagat
     1561 aagcgatgcg ctgttgaggg tggaggagtt gccggacatg gccacgcgca acatcgccgc
     1621 cgccgccatt gagcgcctgg tggaccgcgc attgatgctg cgtgcccgca cgctggggcg
     1681 cccattccgg ccatccatca tcaccggcgc atccatgtcc gtggaacgca tggaacgctt
     1741 gcaacaacag gcggaggagc ggcgggaggc caaggaggag cggcgccgga agaaccggga
     1801 ggagtgggcg atgctcaacc gcgccacagg gcatgcgtcc aagtggtggt tacaaagaaa
     1861 tgagtgaggc atggaacagt atgacgacct gttgcttgcc ctgtccgatg cggggatgcc
     1921 cctatcgcaa gaggaccgcg accgcatcgg tgagcgcgcg ggagccccgc cgccggacag
     1981 ggcgcaggac atcgaccgcc caggcctcat catggatggg cgtcagcacc tgtggctgac
     2041 cccccagcag gcccgtgcgg cgcgggaacg gagcgagaac ccgcagccgc gcgcggaatg
     2101 ctacctggac cgcataccgc aggcgtggaa ggatgagtgg cgcgaccgtg agataagccg
     2161 cgatgactgg tgccccatgg acgccaagac cctcgcccgc aacgatgagt tccgagcgtt
     2221 ccacctctcg cacatccccc ggttcgaccg catcgcgccc tatgagccct tctggctctt
     2281 catcgagcag gccctgcgct ggctggccga ggatacctcc ctctacgatg tgcgggccga
     2341 ggaccgctat gccttcgtcc aggaggagtt ccgccgcatg gatgagaacc acttgtacgg
     2401 cgcgctgaag tacggctaca tccccgatga cagcatgccg ggcggagttc gcaagtacga
     2461 tgcctccacc ccgcaggcgc tcatactcta ctggtgcgcg gtcgggctct ccggagaact
     2521 gttcaagggg cgccagtccg ccgtgacatc caccttcgag ttggacgagg ccatatctat
     2581 cctcaccacg ccatcatcct ccgccgtgct ggtgacggac gacgtgaagt acaccgggga
     2641 gaagatcttc aagcagaagt tcaagtccat gatctccctg atggtggatc gcaacccgtg
     2701 gatggacgcc gacaagtggc tgtggagcgc ttcgtccgtg accgccacgt ggaccgagcg
     2761 cgaccggaag ggggccagca acgcgatgtc cgccgagcgc acggtggcgg catcgggcga
     2821 tacgcaggtc atcaacggta ccaacgtatc gcgcatcaag atcgacgagg cgcagaatgt
     2881 cggcccctac tcgggcatca agcgcgaggt gcgcccggcc atggtgtcat tcgttgacgg
     2941 cgttgcccgc atcaagcgca gtctctgggc ttacgggacc ggaaatgcca aaaactcggg
     3001 atccgagttc acatcggaat acaaggcgac gatggaggct ttccgaaagg gaagggatac
     3061 gtccacctac gtcccgggat tcttcgactg gacatgccga ccgggcatga cggaaaaaga
     3121 tctgatcgaa gagtacgact tcttcatgaa cgcttcggag ggggacatgg cctcccgttc
     3181 gtccgaggag cggctggcga tgttctactc cgcccacccg aacaagcccg aggatgccta
     3241 cctgaccctg caccggcacg tggttcccgt gacggtgatc gccgaagcgt gggaactgat
     3301 cgaacgccat tgcacaaagc gcggcatccc cgtgcttgga catttcgatc cggtgttcaa
     3361 cacgagcgtt cccatggccg aaggctcgtg gtacccgcac ctcgtggtgg ggtccaagtg
     3421 ggtgccccat gcgccggatt cattgaacag cccgtgccgg atgttcctgc ccaagaagag
     3481 cgccgaacgc tgggtatacc ggtgggcaca gggcaccgat cccgctgtcg tggggtccgg
     3541 ctcatccatg ttcgcatcag cgatcttcga cggcgccggg gcttggcgcg accgcgacgg
     3601 tgcgcgcacc ttcaacccca cggtggcatg cctgctcaac gacagcaagc agaacatgga
     3661 ggagtgcttc aaccagagca tcctcatggg tatgtactac gccaacgacg ggcagggggc
     3721 atgccccgaa ctggtcgagg ccaaccgggg aggcgagtac acgcgctgga aatcatcatc
     3781 catgttcaac ctgtcccgct cgctgctata ccgcacgcag atgcccccaa aataccaggg
     3841 cggtggccat ctgtacggca tcgacatgaa gaacaacgat cgcaacgggc gcaagaccga
     3901 actgttcttc gacatcgtgg ccttcatccg ccagtacggc ccccgggcgg acgaatcagg
     3961 gaagaagcgc ctgctgaata tctggtatta cgactactgg acgcagctct acaacatcga
     4021 agtggacgaa caggccacag gcgccatcaa gttcggcacc cggagcaaga acctctacaa
     4081 cgatgacatc gtgtacgccg tccagtacgc caacatcgcc cacaccgtgt acgctggggc
     4141 gcacacgccg atgatgatca acacccacgc cccggagttc cgcgtggtgc gaaagcccgt
     4201 gcgcgacatg aacacgcttg aagtgcgcta cgaggaggtg cgcgaacccg tcatctacgt
     4261 ctgacatggc catacaggac cgctaccttg acgtgcttgc cgaactggcc aagaagggct
     4321 atctcgtcca cgcgcccatc agcgccaagg gactgcggca ggactacccc gaactgaacg
     4381 acgtggcgga gttcaagccg ctgtcatcgg cggaactgct catggtgtgg tggttcgcct
     4441 cacgctgctc ccccatcatc gagatgcccg aggagaagcg catcctcgtg gctatcgacc
     4501 agtccttccg ctcccccgcc atggccgagg cgcgcaagat cgagtacggc acgctttcgt
     4561 tcaccaacga caacatcaag gccgccatca agcgcatgga tgccttcgat cccgggggcc
     4621 gcatcaccgc catggctgac gacctgcacc tgctgcggca gtgccagacg gtgatccgca
     4681 aggacatttc caaggcggac atggaggaga tcaccgaatg gatgaaggcc gtgagcacag
     4741 cgcgaaaact gcgtgacgag atcctccagc gcatcgagcg cggaagcctc ggcgtggccg
     4801 atgagaccac cgtttccgcc tcactcaaag gcgccgcccg cgagtacatg aagaacaaga
     4861 cgaactgatc ccatgagcac gctgaacaac atcatgtggg cgtggcccag catgtcgccc
     4921 gtgagccgcc cgcccatgga cataccgtgg gagaagaagg acgaggcgta cgacgacacc
     4981 gtgatgcgct acttcctgag cgggatgcca tccgtgtacc gggacagcta cctcaaagag
     5041 tacgccgcga acctggccat ggtgcagaat gaaccgctct ccttcgggtc gccgcagatg
     5101 atccgcgagt tcctgggcga cgagaacaac cccacgggac ggttggcgtt gcgctacgcc
     5161 atcgggcagg ccatgcacac gcggctcgta ggtgtggccg aatccatcag cgtgcatgcc
     5221 cgcgcggaag gctggacatc gaacgtggcc gcccgcaagg atgcggccct gtatgaacgg
     5281 ctgctgaagt cccagatggc gcaggctggc tccccgctga ccgaggccat ggcgcaggtg
     5341 ggcctatcgc cagacgagga cggcgagcgg gaccggcacg agaacctgtg gacagacccc
     5401 tatgagagcg cgatcagcaa tctcatcgcc gccatgggtg agcggcagaa gctcaccagc
     5461 acgatccgcc tggccgcctc gcacctcagc ctgtcgggcg tggtggccac gcaccactac
     5521 aacaacggca accacatcga gcgggagctg tgcgagcccg ccgaagtggg ctgggacccg
     5581 tcggccacgc gggaaaacct cagcgacggg gagttctgct atgtggcccc gctcatggac
     5641 gtgtc
//
LOCUS       DAOQOC010000035         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5732_length_5640_cov_3.338944, whole genome shotgun sequence.
ACCESSION   DAOQOC010000035 DAOQOC010000000
VERSION     DAOQOC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5732_length_5640_cov_3.338944"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PL106_00995"
     CDS             complement(<1..466)
                     /locus_tag="PL106_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31670.1"
                     /translation="MRGVVLLGVVLGLQPGVQAQLRNANWFFNSTNLLSYASGAPVAS
                     TAGPLIGGSTAGLSDPQGNALMYAVGNAVLNREMNPMSGSPSLNNATANQGYLFLPVP
                     GDPSGSVVFHNRGINFSSTLSYGYLDMDLDNGSGGFVGGMTVLASDATCKLTA"
     gene            complement(1032..2948)
                     /locus_tag="PL106_01000"
     CDS             complement(1032..2948)
                     /locus_tag="PL106_01000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA31671.1"
                     /translation="MKLSVIIVNYNVRAYLEQCLRTVFEALEGVDGDVFVVDNQSTDG
                     SVEMVREKFPGVRLIANKENVGFSRANNQAIRESSAEYVLLLNPDTVVGEDVFRTVIT
                     FMDKRPACGGLGVKMIDGAGRFLPESKRGLPTPAVAFYKIIGLTRLFPRSKVFGRYHL
                     GHLPENGAAPIEILSGACMFLRKRTLDEVGLLDESFFMYGEDIDLSYRITMGGYENWY
                     LPEARIIHYKGESTKKSSVNYVFVFYNAMAIFAKKHFTRKRPDVFAGLINGSIYLSAA
                     GAIVTRFLRRALLPMLDVLMVSILLIAGQYMLGGIAPDAGKLRFFVATGAGCALLSAF
                     FGVYDLPVRLINVLKAVAAMVLVGGGVWLLNGEDRTQGLMVGYLVAVCGISLVATRLL
                     LHLFRVKPFTLRALDRKRVLAIGSAEESQHALALLWQTHFGLGRQQQMDPKEANAADA
                     VEAIRKKIRKHGIDEVVFCAKDLAWGRIVELMERLKRTRVMFKIAQPAREFIIGPSSI
                     ESVQDLLIMEEHAVSSSAARRRKRILDVAVSCILLLLLPITIWFVRDKSGFLGNIVRV
                     LRGSASWVGYFPHMDQGVRLPRIRPGILDPVRADGLDPVPLTVHRVNLTYAKDYRTGQ
                     DLRLIWRGFAGLGG"
     gene            complement(3067..3684)
                     /gene="recR"
                     /locus_tag="PL106_01005"
     CDS             complement(3067..3684)
                     /gene="recR"
                     /locus_tag="PL106_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNA31672.1"
                     /translation="MSSSSQLLETAVDQLATLPGIGRRTAMRMALDLLRREPHEVTRF
                     SEAVRRLRDEVRYCTVCCNISDQPVCGICANPARDRSTVCVVEDIRDVIAIENTRQFK
                     GLYHVLGGVISPMDGLGPDDLNTRQLFERVGRGDIVEVVLALGATLEGDTTGFYLHRK
                     LKEQGVEVSQIARGISIGGELEQVDEVTLGRSIADRKPYEQGVRR"
     gene            3822..5150
                     /locus_tag="PL106_01010"
     CDS             3822..5150
                     /locus_tag="PL106_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNA31673.1"
                     /translation="MRSILILGAGRSASSLIQYLIDHAREQQWRVTVADKDADHARKL
                     TGTANDAAEGIALDATDADARAMLIAKHDLVISMLPAFMHMDVLKDCLRLRKHVITPS
                     YVPDALWALDAEFKQAGLIVLNEMGLDPGIDHMSAMRILDRIRAMGGTMEAFESYCGG
                     LVAPESDDNLWGYKFSWNPRNVILAGAGGSAKYIENGITKYIPYHKLFQRTSRIDVPG
                     FGAFDGYANRDSLKYRSHYGIDGIPTMKRGTLRKGGFCTAWDCFVQLGCTDDGFIMEL
                     GTDATWNDYIHAFLPSVADRDARAGLAHYLGLEPEGTVMKMLDELRIFSNERIGRTGL
                     SPAGLLQHLLEQRWVLGPEDKDLVVMWHRFSYSHEGTSHGIHASLVVVGQDQVHTAMA
                     RTVGLPIAMACKLVFNNGINDRGVLLPLKPGIYLPVLDELERIGVVFHER"
     gene            complement(5159..>5640)
                     /locus_tag="PL106_01015"
     CDS             complement(5159..>5640)
                     /locus_tag="PL106_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31674.1"
                     /translation="TENVAASRWADRIRVHRMDARRMQASEPYDVIICNPPFYPGEMG
                     SLDERVELAKHAAGLAFHELLELVDRVLAPAGRLNVILPRNRVLDFDGDAGQVGLSPS
                     RRCDVRYLASRPPKRVLMELKRGEQATTSEEITVEKSGPFDYSPEYRALLADLMLKF"
BASE COUNT         1096 a         1825 c         1637 g         1082 t
ORIGIN      
        1 ccgccgtcaa tttgcacgtg gcatcgctcg ccaacaccgt catgccgcca acgaagccac
       61 ctgaaccgtt gtccaggtcc atgtcgaggt agccgtatga aagggtcgat gagaaattga
      121 tccccctatt gtgaaaaacg accgatccgc tgggatcccc tggcacgggc aggaacagat
      181 aaccctggtt cgctgtggcg ttgttcaggg acgggctccc gctcataggg ttcatctccc
      241 tgttcagcac ggcattgccc accgcgtaca tcaatgcgtt cccttgcgga tcgctcaacc
      301 cggcggtgct gccgccaata agggggcccg cggtcgaggc cactggtgcg ccactcgcgt
      361 aggacaacag gttcgtcgaa ttgaagaacc agttcgcatt gcgcaactgg gcttgaacac
      421 ctggctgaag gccaagaacc accccaagca ggacgacgcc gcgcatgatc cggtcaatat
      481 atgcgccagc catggttggg atcctggttg ccatacagtc cgacggtctc ttcacaacat
      541 tcgccctccg tggtaccgga tgcgcatacc cataccgcga tcggtgtacc tgagcatcca
      601 gtgccgcagt acaggtcgat ctggtacacg ttggggtacg gcgctggcac agtgaaggag
      661 gcggatcctg gaccggtcaa attcacgcac tgagaggcgt acggtccggc tgttgacgga
      721 caattgcccg acttggacca gaccttgacg gagaggtcgc agtccaattc tgtcgcgacc
      781 tgaagatcca atgtttgagc gttggttgcc gtgaccatgc acagactgcc taccaagaga
      841 tgtgtgcgcc atttaaggga tctcatagtt ctcatggctg ttcatgtttc gagtacacga
      901 aggtatggtg caagtaaaat gatccgataa gcctgttgaa aactaccgca tacgtcttcg
      961 agaatccgcc gtccacttgt acctgctggg gcgctccctt catcaccaca tgataataga
     1021 ggtttcccga cctacccccc aagccccgcg aatcccctcc agatcaaccg cagatcctgc
     1081 ccagtgcggt aatccttggc gtaggtgagg ttgacgcggt gcaccgtgag cggcacgggg
     1141 tcgaggccgt cggcgcgcac aggatcaagg atgccgggtc ggatccgtgg caggcgtacg
     1201 ccctgatcca tgtgcgggaa gtagcccacc cagctcgccg atccgcgcag cacgcggacg
     1261 atgttgccga ggaagccact cttgtcccgc acgaaccaga tggtgatggg gaggagcagg
     1321 agcaggatgc aggagaccgc gacatcgagg atgcgtttgc ggcgacgcgc ggcggaactg
     1381 ctcacggcgt gctcctccat gatcagcagg tcctgcacgc tctcgatgct gctggggccg
     1441 atgatgaact cgcgcgccgg ctgagcgatc ttgaacatca cccgtgtgcg cttcaaccgc
     1501 tccatcaact ccacgatgcg gccccacgcc aggtccttcg cgcagaaaac cacctcatcg
     1561 ataccgtgtt tgcggatctt ttttcggatg gcctccacgg cgtccgccgc attcgcctcc
     1621 ttcggatcca tctgctgctg acgtcccaac ccgaaatgcg tttgccacag cagcgcgagc
     1681 gcatgctggc tctcctctgc gctgccgatg gcgagcacgc gcttgcggtc cagtgcacgc
     1741 agggtgaagg gttttacccg gaacagatgg agcagcagtc tcgtggcgac caaggatatc
     1801 ccgcacacgg cgaccaggta gccgaccatc aggccttgcg tccgatcctc tccattgagc
     1861 agccagacac caccgcccac caacaccatg gcggcaaccg ccttcaacac gttgatgaga
     1921 cgaacgggca ggtcgtacac gccgaagaag gcggacaaca gcgcacagcc cgctccggtg
     1981 gccacgaaga accggagctt gccagcatcc ggagcgatac cgcccaacat gtattgtccc
     2041 gcgatcagca ggatggatac catcagcacg tccagcatgg gcagcaatgc ccgccgcagg
     2101 aagcgcgtga cgatggcccc cgcagcgctc aaatagatac tcccgttgat gagccccgcg
     2161 aacacgtccg gccgcttgcg cgtgaagtgc ttcttggcga agatggccat ggcgttgtag
     2221 aacacgaaga cgtagttcac gctgctcttc ttggtgctct cgcccttgta gtggatgatg
     2281 cgcgcctcgg gcaggtacca attctcgtac ccgcccatgg tgatccggta gctgaggtcg
     2341 atgtcctcgc cgtacatgaa gaagctctcg tccagcaggc ccacctcatc cagcgttctc
     2401 ttccgcagga acatgcaggc gccggaaagg atctcgatgg gcgccgcccc gttctccggc
     2461 agatggccca aatgatacct tccgaacacc ttgctgcgcg ggaacagccg cgtgaggccg
     2521 atgatcttgt agaaggccac cgccggtgtg ggcaggccgc gcttgctctc gggcaggaag
     2581 cgccccgccc cgtcgatcat cttcaccccc agcccgccgc atgcgggacg cttgtccatg
     2641 aaggtgatca ccgtgcggaa gacgtcctcc cccaccaccg tgtcggggtt cagcaacagc
     2701 acgtattccg ccgagctctc ccggatggcc tggttgttcg cccggctgaa gccgacgttc
     2761 tccttgttgg cgatgagccg gacaccggga aacttctcgc gcaccatttc cacgctgcca
     2821 tccgtgctct ggttgtccac cacgaacaca tcaccatcaa cgccctcgag cgcctcgaat
     2881 accgtgcgca ggcactgctc caggtaggcc cgcacattgt agttgacgat gatgacgctg
     2941 agtttcatgg ccagaggtac tgggtaagca gcaggcagca ggcagcgggc agcaggtaga
     3001 acgcagcgca tggccaacgg ctactgccag ctacctgctg cccgctgcct gctgcctgct
     3061 gccttttcat cgcctcacac cctgctcata cggtttcctg tccgcgatgc tccggcccag
     3121 cgtcacctca tccacctgct ccaactcgcc gcctatgctg atgccccgtg cgatctggct
     3181 cacttcgacc ccttgttcct tcagcttgcg gtgcaggtag aaacccgtgg tgtccccttc
     3241 caacgtggcg cccagtgcga gtactacctc aacgatgtcc cctcgtccga cacgctcaaa
     3301 gagttgccgc gtgttcaggt cgtccggtcc caggccatcc atggggctga tcaccccgcc
     3361 cagtacgtgg tacaggcctt tgaactgtcg cgtgttctcg atggcgatca catcacggat
     3421 gtcctccacc acacacacgg tggaccggtc gcgtgcagga ttcgcacaaa ttccacagac
     3481 cggttgatcg ctgatgttgc agcacaccgt gcagtagcgc acttcatcac gcaagcgcct
     3541 gaccgcttca ctgaaccggg tcacctcgtg cggctcacgc ctgagcaggt ccaacgccat
     3601 gcgcatcgcc gtgcggcgcc cgatgcccgg caaggtggcc aattggtcca cggcggtctc
     3661 caggagttgc gaactgctcg acatgcgcgc aaagatgatc cgtacatgtc cttatttcgc
     3721 ccactccatc cacctgcgcc gaggcttcgg cggataccac ccacctgcgc cgaggcttcg
     3781 gcggatacca ccctccttcg ccaaaggctt cggcgtatac catgcgttcc atcctcatcc
     3841 tcggcgccgg tcgttcggcc tcatcgctca tccaatacct gatcgatcac gcccgcgagc
     3901 agcaatggcg ggtcaccgtg gccgacaagg atgcggacca tgcccggaaa ctcaccggta
     3961 ccgcgaacga tgcggctgaa ggtatcgccc ttgatgccac cgacgccgat gcccgcgcca
     4021 tgctgatcgc caagcacgac ctggtgatca gcatgctgcc cgccttcatg cacatggacg
     4081 tgctgaagga ttgcctacga ctgaggaaac acgtgatcac cccgagctat gtgcccgatg
     4141 cgctctgggc cctggatgct gaattcaagc aggccgggct gatcgtcctg aacgagatgg
     4201 gcctggaccc cggcatcgat cacatgagtg cgatgcgcat cctggaccgg atccgtgcga
     4261 tgggcgggac gatggaagct ttcgagagct attgcggtgg gctcgttgcg ccggagagcg
     4321 acgacaacct ctggggctac aagttcagct ggaatccgcg caacgtgatc ctcgcggggg
     4381 ccggtggttc cgcgaaatac atcgagaacg ggatcacgaa gtacatcccg taccacaagc
     4441 tgttccaacg aacctcacgt atcgatgtgc ccggcttcgg cgcatttgat ggctatgcca
     4501 accgcgattc gttgaagtac cgctcccact acggcatcga cggcatcccg accatgaagc
     4561 gtggcaccct gcgcaaaggc gggttctgca ccgcatggga ctgtttcgtg caactgggct
     4621 gcacggacga tggcttcatc atggaactgg gcaccgatgc cacatggaac gactacatcc
     4681 atgctttcct cccgtccgta gctgatcgtg atgcacgcgc ggggctggca cattacctgg
     4741 gcctggagcc cgagggcacc gtcatgaaga tgctggatga gttgcgcatc ttctcgaacg
     4801 agcgcatcgg ccgcacaggg ctttcacctg cgggcctgct gcaacacctg ctggagcagc
     4861 gctgggtgct cggccccgag gacaaggacc tggtggtgat gtggcacagg ttcagctact
     4921 cacatgaagg tacctctcac gggatccatg cctcgctcgt ggtggtcggg caggaccagg
     4981 tccatacggc catggcgcgc accgtggggt taccgatcgc catggcgtgc aaactggtgt
     5041 tcaacaacgg catcaatgac cgtggcgtgc tgctgcccct gaagcccggg atctacctcc
     5101 cggtactgga tgaattggag cggatcggtg tggtgttcca cgagcgctaa gcatcgggtc
     5161 agaacttcaa catcaggtcg gccaggagtg cgcggtactc gggtgaatag tcgaatggcc
     5221 cgctcttctc gacggtgatc tcctcgctcg tcgtggcttg ctccccgcgc ttcagttcca
     5281 tgagcacgcg tttgggcggc ctgctggcca ggtagcgcac atcgcatcgt ctgcttggtg
     5341 acaggccgac ctgaccagca tcgccgtcga aatcgaggac gcggttcctc ggcaggatca
     5401 cattcagccg accggccggt gcgagcacac gatccaccag ttcgagcaat tcatggaacg
     5461 cgaggcccgc ggcatgcttc gccaattcca cacgttcatc gagcgatccc atttcgccag
     5521 gatagaacgg cggattgcag atgatcacat catagggttc gctcgcctgc atgcgtcgcg
     5581 cgtccatgcg atgcacgcgg atgcgatcgg cccagcggct cgccgccacg ttctccgtgg
//
LOCUS       DAOQOC010000036         5631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5749_length_5631_cov_4.242468, whole genome shotgun sequence.
ACCESSION   DAOQOC010000036 DAOQOC010000000
VERSION     DAOQOC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_5631_cov_4.242468"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PL106_01020"
     CDS             <1..1057
                     /locus_tag="PL106_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31675.1"
                     /translation="QESYEVAQMCLIIAPKEPTGKLVLDADKLRSAFTQNDDGWGFMW
                     LDEKDGKPSLGRMRGLLVDELVAAYDTLKDKPGLHLHLRTKTHGLVDVKNCHPFAVTT
                     DTLLMHNGIFRVDTLLSDRSDTWHYAALISRRFAERGDTNPIYFDEKWQEDTAKFFGT
                     SKGVFLREDGKRWICNEKYGTWYEDQWYSNTGGAPWIEPKYDYSHQSYPMHGYYMSDS
                     IYEDYGDGLGYVRQPISGKHSDRGSLRQPGLAAGWRKQGRGPGCQLCNDGDGTVFCKS
                     CGSFVCGDCRTYWKGFGHLCDRCLSEHAGGTHPIVEEKEGVKDETGTGVGHGAVGQEE
                     QCVQECGGQGSVGGEEK"
     gene            1041..1274
                     /locus_tag="PL106_01025"
     CDS             1041..1274
                     /locus_tag="PL106_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31676.1"
                     /translation="MAKRNEWADDRRSLNVILNVMAFGSISQKYDEALDAWCRVAKAP
                     DVELPDGTSETVSGWAEVAHIFAEVLRKNRKAV"
     gene            1276..1734
                     /locus_tag="PL106_01030"
     CDS             1276..1734
                     /locus_tag="PL106_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31677.1"
                     /translation="MSDYIWRHVGRNRMSAGYSDYLNAMYEGWDEFSLDGDKRVWVVT
                     YEDCGGVKYVRLAGAPFSKYAMDLPRFMRLDAWEDYDVDRDIFTMRVPVSLFVCAHPT
                     IGKRVLGKRSKYFQQCLSLALYAQMMIGGESTKSFPWWRKTGSIDREYER"
     gene            1731..1982
                     /locus_tag="PL106_01035"
     CDS             1731..1982
                     /locus_tag="PL106_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31678.1"
                     /translation="MKINNVTFPRSASDWLKEAARCGAGTRDGSGKAWRVGYRNEFPK
                     RGLDGLDDFMFLAHHGFVKLGDDGQIMVLDIDDNDGIGE"
     gene            1984..2403
                     /locus_tag="PL106_01040"
     CDS             1984..2403
                     /locus_tag="PL106_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31679.1"
                     /translation="MLTDHENCFDQGSLDEARKVLLSRGWRHGFCVLGTDTMHYVCAH
                     DGGEVMIYPHDVQWTGCANVDIGANFVCVSLKTGNMRFNSCFNEKERIQRDAFRASID
                     RDLMLEIAHKVSGHKTKTAEVPWFKKTGNADKEYENA"
     gene            2400..2756
                     /locus_tag="PL106_01045"
     CDS             2400..2756
                     /locus_tag="PL106_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31680.1"
                     /translation="MKISYRVHIDALSKCDLNDEGVCVLPAHWDSEEVYLPDACEKYL
                     YSKMKSSKFSDSDLPVWINEWAKAEGVAIDVKLVDLVSERILRRVKMRKLVAYDTKTR
                     SLEWIGRNTDGLFKDT"
     gene            2734..3003
                     /locus_tag="PL106_01050"
     CDS             2734..3003
                     /locus_tag="PL106_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31681.1"
                     /translation="MDYSKTLDDSRSILNEAMAKAFVRSELTNMDAQQRAEHTIELAE
                     YVALYVEKVLRAVEVTHLIRDTLKDIEKQKELLASTREDDDGPPQ"
     gene            3095..3331
                     /locus_tag="PL106_01055"
     CDS             3095..3331
                     /locus_tag="PL106_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNA31682.1"
                     /translation="MVQKIITLRFDRGLTIIEIAEVLNRGGKRTRTGKRWTYYSVRSV
                     LSKMHIHDEKAYHMGRHMEPTLVVKLPAKEKKDD"
     gene            3324..3812
                     /locus_tag="PL106_01060"
     CDS             3324..3812
                     /locus_tag="PL106_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31683.1"
                     /translation="MTDILKKILEEGYLGKTTDREVAKMYGIDPRTVSTIRTTAGIPV
                     FVNPSTQRTGEVINGVDRARLEKVIKSVEEGVRTFGDRQMAMMFAAKKEGLKVHTVRT
                     YLGWAKSMGLSMSSDIRPKDHKALVSGSASRLDAIEKMLINVATTQTTILSTLRNKGI
                     IE"
     gene            3931..4158
                     /locus_tag="PL106_01065"
     CDS             3931..4158
                     /locus_tag="PL106_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31684.1"
                     /translation="MKTRTLYTEYVGYNPKDKKDIDYARVIAKDRQHGRKNGSSWYAI
                     VGVVLPIDDDKFDDKITGWYEDVYPSAKGVK"
     gene            4247..4657
                     /locus_tag="PL106_01070"
     CDS             4247..4657
                     /locus_tag="PL106_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31685.1"
                     /translation="MTKTPAWLKTYVREIQVRLGRGETDIRIGEHHSDEGTYAQVNPH
                     EQAAEAEIRFSPDFYNDTPQGQRNTVVHEVLHLHFVGVDDVVNAWVDKLAPVDYAMLK
                     RMMVRQEERIVDFMARIISPHMPLPPKRKKRERR"
     gene            4644..5063
                     /locus_tag="PL106_01075"
     CDS             4644..5063
                     /locus_tag="PL106_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31686.1"
                     /translation="MSGDEERFESIHNALRNNPGSIVRALAELNSMGTGVVWTFRGLC
                     DVLGSPEYRSWVDSVRVILMAQAEHDALRKVAFSDNLSDQAQASIAVALLSGKTDDAK
                     KKAGRAKEDRGHGPAKVQTQTEALLEELEEARAEAEE"
BASE COUNT         1313 a         1397 c         1854 g         1067 t
ORIGIN      
        1 acaagaaagc tatgaggttg ctcaaatgtg cttgatcatc gcaccgaagg aaccaaccgg
       61 gaagctggtg ctggatgcgg acaagctgcg ctcagcgttc acgcagaatg acgacggctg
      121 ggggttcatg tggttggacg agaaggatgg gaagccaagc ctggggagaa tgcgcgggct
      181 gctggttgat gaactggttg cagcgtacga tacgctcaag gacaagccag ggcttcacct
      241 ccaccttcgc accaagacac acgggctggt tgacgtgaag aactgccacc catttgccgt
      301 gaccaccgac accctgctca tgcacaacgg gatcttcagg gtggacacgc tgctgtctga
      361 ccggtccgac acgtggcact acgccgccct catcagccgc cggtttgccg agcgcgggga
      421 caccaacccc atctacttcg acgagaagtg gcaggaagat accgcaaagt tctttggcac
      481 cagcaagggt gtgttcctgc gtgaggacgg gaagcgctgg atctgcaacg agaagtacgg
      541 cacgtggtat gaggaccagt ggtactccaa cactggaggc gcgccatgga tcgagcccaa
      601 gtatgactac tcgcaccagt cctaccccat gcatgggtac tacatgagtg actccatcta
      661 tgaggactac ggtgatgggt tggggtacgt gcgccagcca atctccggaa agcacagtga
      721 tcgggggtct cttcggcaac ctggtcttgc tgccgggtgg cgcaaacagg gccgcgggcc
      781 ggggtgccaa ctctgcaacg acggggacgg gacggtcttc tgcaagtctt gcggctcgtt
      841 tgtttgcggg gattgtcgga cttactggaa ggggttcggt catctctgcg accgatgcct
      901 cagtgaacac gcgggcggga cgcatccaat cgtcgaagaa aaggaagggg tgaaagatga
      961 aaccggaacg ggtgttggac atggtgctgt gggccaagaa gaacagtgtg tgcaagaatg
     1021 cgggggacaa ggcagcgtgg gtggcgaaga gaaatgagtg ggccgatgac cgtcgcagtc
     1081 tcaacgtgat cctcaacgtg atggcgttcg ggtccatcag ccagaagtac gacgaggcgc
     1141 ttgatgcgtg gtgccgtgtg gccaaggctc cggatgttga gctcccagat gggacgagcg
     1201 agacggtgag tggttgggcc gaggttgcac acatctttgc agaggtactg cgcaagaaca
     1261 ggaaggcggt gtgacttgag cgactacatc tggcggcatg tcggaagaaa cagaatgtca
     1321 gcgggataca gcgactacct caacgccatg tacgaagggt gggacgagtt ctccctcgat
     1381 ggcgacaagc gcgtgtgggt ggtgacgtat gaagactgcg gtggtgttaa gtacgtcagg
     1441 ctggccggcg ccccattcag caagtacgca atggaccttc cgagattcat gaggctcgac
     1501 gcctgggaag actacgacgt cgaccgggat atatttacca tgcgtgtgcc tgtgtcactg
     1561 ttcgtttgcg ctcacccaac cattggaaag agggttctcg gcaagcgcag caagtacttc
     1621 cagcagtgcc tgagccttgc gctgtacgca cagatgatga tcggcggaga atcgaccaag
     1681 tcattcccgt ggtggagaaa gaccgggtcg attgacaggg agtacgagag atgaagatca
     1741 acaacgttac gttcccgcga tctgcttctg actggctgaa ggaggcggcg cggtgcggtg
     1801 ccgggacaag ggatggcagc ggcaaggcgt ggcgggttgg ctaccgcaac gagttcccga
     1861 agcgggggct tgatgggctt gatgacttca tgttccttgc gcaccatgga ttcgtcaagc
     1921 ttggtgatga cgggcagatc atggtgcttg acattgacga caacgacggg attggagagt
     1981 gagatgctga ccgatcacga gaactgcttc gaccaggggt cactcgacga ggcaaggaag
     2041 gttcttctgt ctcgcggatg gagacacggg ttctgcgtcc tgggaacaga caccatgcac
     2101 tacgtgtgcg cgcacgacgg cggggaggtg atgatctacc cacacgatgt gcaatggacg
     2161 ggctgcgcca acgttgacat cggcgccaat tttgtttgcg tgtccctcaa gactggcaac
     2221 atgcggttca actcctgctt caacgagaaa gagcgtattc agcgtgatgc gttcagggcg
     2281 tccatcgaca gggacctcat gctagagatc gcgcacaagg tatccggcca caagacaaag
     2341 acagccgagg ttccatggtt caagaagacc ggcaacgcag acaaggagta cgagaacgca
     2401 tgaagatctc ctaccgtgtg cacattgatg cgttgagcaa gtgcgacctg aacgatgagg
     2461 gcgtgtgtgt gctgccggcc cactgggatt cggaagaggt gtacctgccg gacgcatgcg
     2521 agaagtacct gtacagcaag atgaagagca gcaagttctc tgactctgac ctgccggtgt
     2581 ggatcaacga gtgggccaag gcggagggtg ttgccattga cgtcaagctt gttgacctcg
     2641 tctccgaaag aatcctgcgc cgggtgaaga tgaggaagct ggtggcgtac gacacaaaga
     2701 caagatccct tgagtggatc gggaggaaca cagatggact attcaaagac acttgatgac
     2761 agccgcagca tcctgaacga ggcgatggcc aaggcgtttg tgcgcagtga gctcaccaac
     2821 atggacgcac agcagcgggc cgagcacacc attgaactgg ctgagtatgt ggccctgtat
     2881 gtggagaagg tgctgcgtgc ggttgaggtg acgcacctga ttcgcgacac gctcaaggac
     2941 attgagaagc agaaggagct tttggcctcg actcgggagg acgatgatgg gccgcctcag
     3001 tgacgtggca aacgggctgt acggtgtggc cccggcgccg tatgggtggg tgaatgtggg
     3061 caagaccatt cggcatcatc acacagagtg gccggtggtt cagaagatca tcacactccg
     3121 gttcgaccgt ggcctcacca tcattgagat tgccgaggtg ctgaaccgtg gtggcaagag
     3181 gaccaggaca ggcaagagat ggacatacta cagcgtgcgt agcgtcctct cgaagatgca
     3241 catccacgat gagaaagcgt accacatggg caggcacatg gagccaacgc tcgtggtcaa
     3301 actaccagca aaggagaaga aagatgactg acatcctgaa gaagattctc gaagagggtt
     3361 acctgggcaa gacgactgac cgggaggtgg ccaagatgta cggcattgat cctcgcacgg
     3421 tgtccaccat ccgtaccacc gccggcattc ctgtgttcgt caacccttcc acccagcgca
     3481 ccggagaggt gatcaacggg gtggaccgtg cccggctgga gaaggtgatc aagtccgttg
     3541 aggaaggggt gcgcaccttc ggagaccggc agatggccat gatgtttgcc gccaagaagg
     3601 aagggctcaa ggtccatact gtgcgcacct acctcggctg ggcgaagagc atgggcttga
     3661 gcatgagcag tgatatccgg cccaaggacc acaaggccct cgtctccgga agcgccagcc
     3721 gcctcgacgc catcgagaag atgctgatca acgtggccac gacgcagacc accatccttt
     3781 ccacgctgcg caacaagggg atcatcgaat gagcgggcca atgaagaatc tgctgggcgc
     3841 attgttcgcg gctgccttgg tgattgggct ggtggccgtg tgcgtgcgag cgccgggctg
     3901 tggtgcaacc atctaccagg aggacaagtg atgaagacgc gaacgctgta caccgagtac
     3961 gttgggtaca acccaaagga caagaaggat atcgactacg cccgggtcat tgcgaaggac
     4021 aggcagcatg ggaggaagaa cgggtcgtcg tggtacgcca tcgtcggagt ggtgcttcca
     4081 atagacgatg acaagttcga tgacaagatc actggttggt acgaggacgt gtaccccagc
     4141 gccaagggag tgaagtgaca ctacatgtat tgtaatgcct cggtgagcgg agtggcatcg
     4201 cagcgcttgg ttcgattcca agacgaggca acagtatact ggtggcatga caaagacacc
     4261 ggcatggctg aagacatacg tccgagagat tcaggtccgt ctgggcaggg gcgagaccga
     4321 catcaggatc ggtgagcacc actccgatga gggcacgtac gcccaggtga acccacatga
     4381 gcaggcggca gaggcggaga tcaggttcag ccctgacttc tacaacgaca cgccgcaggg
     4441 gcagcgcaac accgtggtgc atgaggtact ccaccttcat ttcgtcggag tggacgacgt
     4501 ggtgaatgcg tgggttgaca agcttgcccc cgttgattat gcaatgctca agagaatgat
     4561 ggtgcggcaa gaggaacgca tcgttgattt catggcgcgc atcatttctc cgcacatgcc
     4621 actgccaccg aagaggaaga aacgtgagcg gcgatgagga aagattcgag agcattcaca
     4681 acgccctgcg caataacccg ggaagcattg tacgtgccct ggcggaactc aactccatgg
     4741 ggacgggagt ggtgtggacg tttcgtggtc tctgtgatgt tctcggttct cccgagtatc
     4801 gtagctgggt ggattctgta cgggtcatcc tcatggctca ggcagaacat gacgcgcttc
     4861 ggaaggtggc tttctcggac aacctcagcg accaggcaca ggcttctatt gccgtagcac
     4921 ttctctctgg caagaccgac gacgccaaga agaaggcagg ccgggcaaaa gaagaccgtg
     4981 ggcatgggcc agcaaaggtt cagacacaga cagaagcgct gctcgaagaa ctggaggagg
     5041 cacgggctga ggcagaggaa tagggcgctg cttctggagt tcctgctgtc gaacccgtgc
     5101 ttccattgtg gggaggacga catactcgtg cttgagtttg accacatgca cgacaagcac
     5161 gcacacgtag gcaggatggc gcttgacagg gttggcgttg tcaggatcct gcgggagatc
     5221 ttcaagtgca gggtattgtg cagcaactgt cacacaaggg caacgcgcaa ggataacaac
     5281 gacttcaggt ggaggttctc aaatgagtga tacggaaaag aagatcatgc ggtggtatca
     5341 catcccgttt gttgtgttga tgcttccgat tgcgctgatc cgtatctgga ttgacaagcg
     5401 gaggaagtgg ccgactgatg ggtgagatgc tgttgctgct ggtcctatac tgccccgagg
     5461 actggcaatg gctggtggtt gagccagctg ctgaacactg ggggcctgcg atagtgaggg
     5521 cgttatgaag ggctggttgc ggaggcgtgc ggttgaggtg gtggttgcaa ctgtgctgga
     5581 ggctgtgccc aagattgcac gggcggtggt ccgtgagtgg aggaagcgtc a
//
LOCUS       DAOQOC010000037         5598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5798_length_5598_cov_4.304348, whole genome shotgun sequence.
ACCESSION   DAOQOC010000037 DAOQOC010000000
VERSION     DAOQOC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_5598_cov_4.304348"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PL106_01080"
     CDS             complement(<1..303)
                     /locus_tag="PL106_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31687.1"
                     /translation="MASILPATTAAQCDGCIANVACTADPAYPALCPTQPPDATAGEP
                     YSADITFWLPVNFTDPGTGFNVDFMLMTITGVTGLPYGLDITYSEPSGVYHPQENPY"
     gene            complement(393..1022)
                     /locus_tag="PL106_01085"
     CDS             complement(393..1022)
                     /locus_tag="PL106_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31688.1"
                     /translation="MSALAQLATSLGRVDELPNIALAESIAAEKNMAAIRELVDVLNG
                     KDKALKSDALKTLYEVGYRAPELIAPFIAQFKNLLANPDNRLVWGAMCAIDGIATIKP
                     DAVFMILPQIMAAVDRGTVITRDHAVKTLAKLAAQERFARTAMPLLLEQLRTAPVNQF
                     PMYAELVAAIVFPQDVNNMRRILEARLPELPNEAKKKRVEKALKKLRTA"
     gene            complement(1156..1548)
                     /locus_tag="PL106_01090"
     CDS             complement(1156..1548)
                     /locus_tag="PL106_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNA31689.1"
                     /translation="MAHDPLFEQRIAEALKAHGAKAEPKKMFGGVAFMVNGHMSVGIT
                     NKGDFMARFDGARHEEVLKWPGAKPMTFGKAGMKGFLFVDADAVSTKAALDKWIKLSL
                     AYVNALPKKRAKGAIKKEASGKKVVRKK"
     gene            1611..1844
                     /locus_tag="PL106_01095"
     CDS             1611..1844
                     /locus_tag="PL106_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31690.1"
                     /translation="MGKLQDRKTELVERIQKVRSKKILDAVEQALSGEVEPELTDEEY
                     AEMLEIRRRYDKGEAKGSTWPEVKKRLLRDLRS"
     gene            complement(1992..3167)
                     /locus_tag="PL106_01100"
     CDS             complement(1992..3167)
                     /locus_tag="PL106_01100"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HNA31691.1"
                     /translation="MRSAAFGSGILLLLVPEVLRVYYIMPFPGSQEDVATDLRQVHIA
                     YWLHTNIWWVRLIGLALLIGPFIHYMRLGNVWKRIGILLPTALCALAFYLFNFKLMAD
                     KMFYPPKEKTLTPVPSSGERGAMPDSVANHLAIVVERNGDARAYPIELIGYHHQVLDT
                     IGGEPVMVTYCTVCRTGRAWKPLVNGQPETFRLVGMDHFNAMFEDSRTGSWWRQATGE
                     CVVGELKGQHLEEVPSQQMTIAEFASQHPKGLVFQPDPNFTEQYAGLEGFDDGTIGSS
                     LEYRDSASWQPKSWVVGVVHKGLARAYDWNDLVQRSTLNDTLGGDPITLTSTGSSPGF
                     HVSIDLRDTVWTTADSAGILVGRYQIAGNLRRIPAYQEFWHSWRTFHPNTTRYELKR"
     gene            3293..3622
                     /locus_tag="PL106_01105"
     CDS             3293..3622
                     /locus_tag="PL106_01105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA31692.1"
                     /translation="MAKTSTPAPAELTELPLMDEKTTRQFVLKVGDQRARIEYDHQGD
                     RIFLTRTDVPKQLADCDAHRVLTEKTLSHVEAQKLKLIPLCPKVKDFLRQNPAWQRLL
                     LKGVQLR"
     gene            complement(3667..4215)
                     /locus_tag="PL106_01110"
     CDS             complement(3667..4215)
                     /locus_tag="PL106_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31693.1"
                     /translation="MMIQRLLPVAALLALPVLGLNAQCASMHLEDKDLYDVLRKSPSL
                     STMKAASRMELVNDRYEPCKRRKDARYIRTITPDKHGYTVHVRTRHGFTLMTGHFADA
                     AALVPHGDFRYFDDAGILRAQGRYVDGRKYGIWQRYDDRGQRLSDKEYDGLDWEDKQL
                     KLGLTSQCCYLEEMATRTPEGP"
     gene            4423..>5598
                     /gene="gltX"
                     /locus_tag="PL106_01115"
     CDS             4423..>5598
                     /gene="gltX"
                     /locus_tag="PL106_01115"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNA31694.1"
                     /translation="MTVRVRFAPSPTGPLHMGGVRTALYNYLFARKHGGQFLLRVEDT
                     DQTRYVPGAEDYIKESLAWCGLVPDESPWAGGPDGPYRQSDRKHLYKAYADELIAKDK
                     AYYAFDTPEELDAMRERLQAAGVAAPAYNAVTREHMKNSITLGADEVKERIARGDAYV
                     IRLKVPRHVEVRFEDIIRGWVTVHSANIDDKVLFKSDGMPTYHLANIVDDHLMRITHV
                     IRGEEWLPSAPLHVLIYEAFGWERPAFAHLPLILKPDGNGKLSKRDGDRLGFPVFALD
                     WTDPVTNEKSSGYRERGYYPEAFINMLALLGWNPGTDQEIMSVDELVRLFDLDRVHKG
                     GARFDPEKTKWFNQQYLRMRPDAELGAQLLELLREQGIGTTLDRAIRAAALLKERATF
                     "
BASE COUNT         1079 a         1614 c         1785 g         1120 t
ORIGIN      
        1 atacgggttc tcctgtggat gatagacgcc agatggttcg ctgtacgtga tgtccagtcc
       61 gtacggcaga ccggtcaccc ctgtgatggt catgagcatg aagtccacgt tgaagccggt
      121 tccggggtcg gtgaaattca cgggcagcca gaaggtgatg tcggcgctgt agggttcacc
      181 ggcggtggcg tcaggcggct gtgtggggca gagcgcaggg taggccgggt cagccgtaca
      241 agccacgttc gcgatacaac catcgcattg ggccgcagtg gtcgctggga ggatactggc
      301 caaggcagca atcccgaata tccagtagca gaatggaagg aggggtgcag gggtacggtg
      361 cggcatcgcc gccaaactac gccgggcacg gatcaggccg tgcgcaactt cttcagcgct
      421 ttctccacgc gcttcttctt cgcctcgttg ggcaattcgg gcaggcgcgc ctccaggatc
      481 cggcgcatgt tgttcacgtc ctgcgggaag acgatcgccg cgaccagttc agcgtacatg
      541 gggaactggt tcaccggtgc ggtgcgcagt tgttcgagca gcagcggcat ggccgttcgg
      601 gcgaaacgtt cctgggcggc gagtttggcc agtgttttca cggcgtggtc gcgggtgatc
      661 accgtgccgc ggtccacggc ggccatgatc tgcgggagga tcatgaacac ggcgtccggc
      721 ttgatcgtgg ctatgccgtc gatggcgcac atggcgcccc acaccaggcg gttgtccggg
      781 ttggccagca agttcttgaa ctgtgcgatg aagggtgcga tgagttccgg cgcgcggtag
      841 cccacctcat agagcgtttt caatgcgtcg ctcttcaggg ccttgtcctt gccgttgagc
      901 acgtccacca gttcgcggat ggccgccatg ttcttctccg ctgcgatcga ttccgcaagg
      961 gcgatgttcg gcagttcatc cacgcggccg agggaagtgg ccagttgtgc gagggcgctc
     1021 atcggtagag cggcccattg gatcgggccg tgggcccgct catacggacg tcctttgttt
     1081 cgggtttcgc gagcgcagcg atcaaaaatt gcgagcgaag cgaagcgatc tgtaattcgc
     1141 gagcgaagcg agccgttact tcttccttac aaccttcttc ccggacgctt ccttcttgat
     1201 agcgcctttc gccctcttct ttggcaaggc gttcacgtac gcgagggaga gcttgatcca
     1261 cttgtcgagc gcagctttcg tgctcacggc atcggcatcc acgaagagaa agcccttcat
     1321 cccggctttg ccgaaggtca tcggcttggc cccgggccac ttcaggacct cctcatgccg
     1381 cgcgccatcg aaacgcgcca tgaagtcgcc tttgttggtg atgccgacgc tcatgtggcc
     1441 attcaccatg aaggctacac cgccgaacat cttcttgggt tcggctttgg cgccgtgggc
     1501 tttcagggct tcggcgatgc gttgttcgaa gagggggtcg tgggccatgg gatgggggtt
     1561 atccgccgga cttcaccggt atcgcgaagc cgcgcaatta tatttggttc atgggtaagc
     1621 tgcaggaccg taagaccgaa ctggtggaac gcatccagaa ggtgcggagc aagaagatcc
     1681 tcgacgcggt ggagcaggcg ctctcgggtg aggtagagcc ggagttgacg gatgaggagt
     1741 atgcggagat gctggagatc agacggagat acgacaaggg cgaagccaag gggtccactt
     1801 ggcccgaggt gaaaaagcgc ctgctgcgtg atctgcgttc ctgatgatcc gattcaccat
     1861 tctgccagag gccgaagagg acacgagacg cgccgttttc gcatctattt cgatgcggaa
     1921 gtcgacgaga tcgtggtcat ccgcgtcttc catctgaaac gtgaccgcaa gacgttgttc
     1981 aagcggcggc gctaacgctt aagctcgtac cgcgtggtgt tcgggtggaa ggtgcgccag
     2041 ctgtgccaga actcctggta agcggggatc cgacgaaggt tgcctgcgat ttggtaccgg
     2101 ccaaccagga tgccagcact atcagcggtg gtccataccg tatcgcgaag atcgatggat
     2161 acatggaacc ccggggagga tccggttgaa gtgagcgtga tgggatctcc gccgagcgtg
     2221 tcgttcaatg tgctgcgttg gaccagatcg ttccagtcgt aggcccgcgc cagccccttg
     2281 tgtaccacgc ccaccaccca gctcttcggc tgccagcttg cgctatcacg gtattccaac
     2341 gatgacccga tggtcccatc gtcaaagccc tcaagtcccg cgtactgttc ggtgaagttg
     2401 ggatccggtt ggaacacgag ccccttcgga tgttgagatg cgaactctgc gatggtcatc
     2461 tgctggctgg gaacttcctc caggtgttgc cccttgagtt cgccgacaac acactccccg
     2521 gtcgcctgcc gccaccagct accggtgcgg ctgtcctcga acatggcatt gaaatgatcc
     2581 ataccgacaa ggcggaaggt ctcgggttga ccgttcacca atggtttcca cgcgcgtccc
     2641 gttcggcaca cggtgcagta ggtgaccatc acgggttccc cgccgatcgt atcgagcacc
     2701 tggtggtggt aaccgatcaa ctcgatgggg taagcgcgtg catcaccgtt gcgttccacg
     2761 acgatggcca ggtggttcgc cacactatcc ggcattgctc ccctctcccc ggaagagggg
     2821 acgggggtga gggtcttctc cttcggcggg tagaacatct tgtccgccat cagcttgaag
     2881 ttgaacaggt agaacgcgag cgcgcacagc gccgtgggca gcaggatgcc gatgcgcttc
     2941 cacacattgc cgagcctcat gtagtggatg aacggcccga tgagcaaggc gagaccgatg
     3001 agccgcaccc accagatgtt cgtgtgcaac cagtaggcga tgtgtacctg tcgcagatcc
     3061 gtggccacat cttcctggct gccggggaag ggcatgatgt agtagacccg gaggacctcg
     3121 ggcaccagga gcaggagaat acccgaccca aaggccgctg aacgcatggt gcaagggtag
     3181 ggcccttcga cgaacaggcc gatttgggga tgcggatgcc ggtgttgggc ggatggaacg
     3241 ggggttcgtg atcgcggatg caagggcgat atctttgtgg ccttctttga ccatggcgaa
     3301 aacctctacc cctgcacctg cggaactgac cgaactgccc ctgatggacg aaaagaccac
     3361 gcgtcaattc gtgctgaagg tgggcgatca gcgtgcccgc atcgagtatg atcaccaggg
     3421 cgaccgcatc tttctgacgc gcaccgacgt tcccaagcaa ttggccgatt gcgatgccca
     3481 ccgggtgctc acggagaaga ccctgagcca cgtggaagca caaaagctga agctgatccc
     3541 gctctgtccg aaggtgaagg atttcttgcg gcagaacccg gcttggcagc ggttgctgct
     3601 gaagggcgta caactgcgct gagcgacgga tcggcttatg gcccggagcg agcggcttgg
     3661 gttggcctat ggcccctcgg gcgtgcgtgt ggccatttcc tccaggtagc aacattggct
     3721 ggtgagcccc agcttcagtt gcttgtcctc ccagtcgagg ccatcgtatt ccttgtcgct
     3781 caaacgttgg ccgcgatcgt cgtagcgctg ccatatcccg tatttgcggc catccacgta
     3841 tcggccttgc gcccgcaaga tgccagcatc atcaaagtac cggaagtcgc catgtggcac
     3901 caaggctgcg gcatcggcaa agtggccggt catcagggta aagccgtgcc gtgttcgcac
     3961 gtgtaccgtg tacccgtgct tgtccggcgt gatcgtacgg atgtatcggg cgtccttgcg
     4021 gcgcttgcac ggctcgtaac ggtcgttcac caactccatg cgcgatgccg ccttcatggt
     4081 ggacaggctt gggcttttgc gcagcacgtc atacaggtcc ttgtcctcca agtgcattga
     4141 agcgcattgc gcgttgaggc caagaaccgg gagggcgagg agagcggcaa ccggtaagag
     4201 tcgctggatc atcatcggct tggttaatat tcaggacaaa tgttgaaaaa gaatcgacga
     4261 accttacaat attttcgacg aacaactttg cttagcgcaa ctgacgagcc cgttttccag
     4321 gtgcgagcgg gcaggtgcgt agcgcggccc gtgtactttc gcgacccttt catgcggcca
     4381 agaccccgcg cagagcaggc aacggccagt attccctgat ccatgaccgt tcgcgtccgt
     4441 ttcgctccga gccccaccgg ccctctgcac atgggcggtg tgcgcacggc cttgtacaac
     4501 tatctgttcg ctcgcaagca tggcggccaa ttcctgctgc gcgtggagga taccgaccaa
     4561 acgcgctacg tgcccggcgc cgaggactac atcaaagaaa gcctggcctg gtgcgggctt
     4621 gttccggatg aaagcccatg ggcaggaggt ccggatggcc cataccgcca gagcgaccgg
     4681 aagcatttgt acaaggcgta cgctgatgag ttgatcgcga aggacaaggc gtactacgcc
     4741 ttcgacacgc cggaagagct ggacgccatg cgcgaacggt tgcaggctgc aggggtggcc
     4801 gcaccggcgt acaacgcggt cacgcgcgag cacatgaaga attccatcac ccttggcgcc
     4861 gacgaggtca aggagcgcat cgcacgcggt gatgcgtacg tgatccgctt gaaagtgccg
     4921 cgccatgtgg aggtgcggtt cgaggacatc atccggggtt gggtaacggt ccacagcgcc
     4981 aacatcgacg acaaggtgct gttcaaaagc gacggcatgc ccacctacca cctggccaac
     5041 atcgtggacg accacctgat gcgcatcaca cacgtgatcc ggggagagga gtggcttccg
     5101 agtgcgcctt tgcacgtgct catctacgag gccttcggtt gggagcggcc cgcgttcgcg
     5161 cacctgcccc tcatcctcaa gcctgatggc aacggcaagc tcagcaagcg ggatggcgac
     5221 cggctgggtt tcccggtgtt cgccttggac tggaccgatc cggtgaccaa cgaaaagagc
     5281 agtggttacc gcgaacgggg gtactacccg gaggccttca tcaacatgct cgcgctgctg
     5341 ggttggaatc ccggtacgga ccaggagatc atgtcggttg atgagcttgt ccggctcttc
     5401 gatctggacc gggtacacaa gggtggtgcg cgtttcgatc ccgaaaaaac caaatggttc
     5461 aaccagcaat acctccgcat gcggcccgat gcggaactgg gagcgcagct gttggagttg
     5521 ctccgggagc agggcatcgg gaccacattg gaccgcgcca tccgggcggc cgcgctgctg
     5581 aaggaacgcg ccacgttc
//
LOCUS       DAOQOC010000038         5590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5809_length_5590_cov_4.471906, whole genome shotgun sequence.
ACCESSION   DAOQOC010000038 DAOQOC010000000
VERSION     DAOQOC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5809_length_5590_cov_4.471906"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PL106_01120"
     CDS             <1..1045
                     /locus_tag="PL106_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA31695.1"
                     /translation="NGYAYVVNGSVYFDVPKFAAKHNYGELSGRRIDELLTNTRDTEG
                     MDEKRSPLDFAIWKKAEAGHLMHWPSPWGEGFPGWHLECSVMSTKYLGMTFDIHGGGM
                     DLKFPHHECEIAQSVAADGQAPVRYWMHGNMLTVNGRKMAKSEGNGFTPEELVTGNHK
                     LLERGYSAMTVRFFMLQSHYASTLDFSNAALQAAEKGLEKMMAAVVTLAALKGGDGPA
                     SAEVAALEQACYDAMNDDLNTPIMIAHLFDGVRMINSANDGKLALSREDIARLNGLFN
                     AMLFDVLGMCKEEEARGDDGALEGVMRLIIEMRSEAKATRNFALSDRIRDQLAAVGIS
                     IKDSKEGSTWDRK"
     gene            1042..2073
                     /locus_tag="PL106_01125"
     CDS             1042..2073
                     /locus_tag="PL106_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNA31696.1"
                     /translation="MKSKGRVRPFIAVGLVATFVGVLVLKSCAPEAPTPAPAPEKPVS
                     VVVPLFDPDSAYGHVAKQVAFGPRVPGSAAHTACADWLVATLKRYGADTVHEQKGTVT
                     AYNGQPVPLRNIIASWDPEKKDRLLLMAHYDTRPFADRDKERPNDPIDGANDGASGVA
                     VWLEVARHFREQAPALGIDLFFTDVEDMGAPNAGGMGARHDENNLESWCLGARYWAKN
                     PHVPGYRARFGILLDMVGSTDAKFPREGISMRIAPQVVAKLWKAAAEAGHGARFLSET
                     KNYVGIDDHLVVFEGTGIPVADIIAWDESSGAFPKTWHTHDDNLANISKESLQAVGAT
                     VMRVVWAER"
     gene            2280..4340
                     /locus_tag="PL106_01130"
     CDS             2280..4340
                     /locus_tag="PL106_01130"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNA31697.1"
                     /translation="MKKTLTLLGLIPALASAQSLVNTTPENRTVLLEDFTGIHCGYCP
                     EGHVIMAALEERFGPRYVGVGVHAGGFAVPQGNEPDFRTDEGTAIDAHFTISGYPAGV
                     INRHLFDGEDDLGRGSWEGAVEQVLDMPSPVNLGVESSYDAGTQTLTVHTHALYTGDS
                     PSGNDYISVLVTESHIIGYQADYTNGTHQNYDHLHVLRGYLTDTWGEDVGNHVAGETV
                     DRTYTMAVPPEWNIANCEVVAFISEYQTEVYQARDVAADGGTTLVIGSMAGNGQPYRP
                     GQDGTATPFDGTFTNLLNADAQYTITLTGDDAPGSWTAGINVDGQDLGNPATVTVTNG
                     ATINVTANITPDAAPGIGDYTLSITSVSNSSAPAISTEYHVISGVHDLIVTHSGAEAH
                     EPIYMNAMYHEPAKAATSKAKFVAFAQADALAGVNNLYVNVSWTFPSLTDDEVAVLAA
                     FMDNGGNLMIAGQDIGWDQSGADGAYGTPATQAFYADHLLADFMDDGSTADNTVNFED
                     ADAVFGTLSNSAVNNVFSTNTYPDRIDPIDPAVPILRYTNPDKIGGLRAEVGNHKVVY
                     FGIGPEQMSNTEVGRGMVQLSHDWFYGIVSVEEFDAAMADLGRPYPSPANDVVNLDVS
                     SIHGATTLEVLDASGRLMLTQAVANNAGLATLHVGSLANGVYSVRARGPQGTGAARAF
                     NVLH"
     gene            4513..>5590
                     /locus_tag="PL106_01135"
     CDS             4513..>5590
                     /locus_tag="PL106_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein Por"
                     /protein_id="HNA31698.1"
                     /translation="MTGRLLPLVALLTTISVHGQIAFGGQPYGMRPTEKTGLPPAPRV
                     IMPTVDAAALMAEDAERAAQGIKGPYRFGFNHATDLSLDNSGIWHTMPNGDRVWRLAI
                     VCPNAFSINFEFNEYMVPEGARVFVYNAWNEVLGGFTAASNGGRPTMGVGQLAGDRIT
                     VEYVEPASVEGQGHLRIGQVTHAYRDVLGLAKGLGDSGSCNNNVICPVGDEWRDEIRS
                     VAIIIVGGSGYCTGTLLNNCAEDGTPYFLTANHCTEGANVNNWVFRFNWESPVCEANQ
                     NGPTNQTVSGASLLENSGGSDVALLQLNSTPPSDYNVYYAGWDNSGAAPTSEVCIHHP
                     SGDIKKISFNNDAAGEADWGSAATW"
BASE COUNT         1125 a         1865 c         1742 g          858 t
ORIGIN      
        1 taacgggtat gcgtacgtgg tgaacggcag tgtgtacttt gatgtcccca agttcgccgc
       61 caagcacaac tacggggagc tgagcggccg gaggatcgac gagctgctca ccaacacgcg
      121 cgacaccgag ggcatggacg agaagcgatc gccgctcgac ttcgccatct ggaaaaaggc
      181 cgaggccggc cacctgatgc actggcccag cccgtggggc gaaggattcc cgggctggca
      241 cctggagtgc agcgtgatga gcaccaagta cctcggcatg accttcgaca tccacggcgg
      301 tggcatggac ctgaagttcc cgcaccacga atgcgagatc gcgcagagcg tggccgccga
      361 tgggcaggca ccggtgcgct actggatgca cggtaacatg ctcaccgtga acggccgcaa
      421 gatggccaag agcgaaggca acggtttcac cccggaggaa ctggtcacgg gcaaccacaa
      481 actcctcgaa agagggtaca gcgcgatgac ggtgcgcttc ttcatgctcc agtcgcacta
      541 cgccagcacg ctcgacttca gcaacgcggc cctgcaggcg gccgagaaag ggctggagaa
      601 gatgatggcg gccgtggtga cactggctgc gctgaaaggt ggggacggac cggcctccgc
      661 cgaggtcgcc gcgctggaac aggcgtgcta cgatgccatg aacgacgacc tgaacacgcc
      721 gatcatgatc gcgcacctgt tcgatggcgt gcgcatgatc aactcggcca acgatgggaa
      781 actcgccctg agccgagagg acatcgcgcg gttgaacggc ctgttcaacg ccatgctctt
      841 cgatgtgctc ggtatgtgca aggaggagga ggcacgcgga gatgacggcg cactcgaagg
      901 tgtgatgcgc ctgatcatcg aaatgcggtc cgaagccaag gccacccgga atttcgcatt
      961 gagcgacagg atccgtgatc aattggcggc cgtgggcatc agcatcaagg acagcaagga
     1021 aggcagcaca tgggaccgga aatgaagagc aaggggcggg tgcggccttt catcgccgtc
     1081 ggtctggtcg cgacgttcgt cggggtgctc gtgctgaagt cctgcgcacc ggaagcaccg
     1141 acaccggcac ccgcaccgga gaagccggtg agcgtggtgg tgccgctctt cgatccggac
     1201 agcgcctacg gccatgtggc gaaacaggtg gcattcggtc cgcgggtgcc cggcagtgcg
     1261 gcccacaccg cctgtgccga ttggttggtg gccaccttga agcgctacgg agcggacacg
     1321 gtgcatgaac aaaaaggtac cgtcaccgca tacaacggcc agcccgtgcc cttgcgcaac
     1381 atcatcgcca gttgggaccc ggagaagaag gatcgtttgt tgctgatggc gcactacgac
     1441 acacgcccct tcgccgatcg cgacaaggaa cgaccgaacg acccgatcga cggggccaac
     1501 gacggggcca gcggcgttgc cgtgtggctg gaggtggccc ggcacttccg cgagcaggcg
     1561 cccgccttgg gcatcgacct cttcttcacc gatgtggagg acatgggcgc cccgaacgcc
     1621 ggtgggatgg gtgcccggca cgacgagaac aacctggaga gctggtgcct cggcgcgcgc
     1681 tattgggcca agaacccgca tgtgcccggt taccgcgcac gcttcggcat cctcctggac
     1741 atggtgggca gcaccgacgc caaattcccc cgcgaaggga tcagcatgcg catagccccg
     1801 caggtggtgg ccaaattgtg gaaggccgct gccgaagccg gtcatggtgc gcgcttcctg
     1861 agcgaaacga agaactacgt gggcatagac gaccacctcg tggtgttcga aggcaccggc
     1921 atccccgtgg ccgacatcat cgcgtgggac gagagctcgg gcgccttccc gaagacctgg
     1981 cacacgcacg acgacaacct ggcgaacatc agcaaggaga gcctgcaggc cgtgggcgcc
     2041 acggtgatgc gggtggtgtg ggccgaacgg tagagcataa gcgggccgtt catgcgagca
     2101 cgggtgccgc accgatttcc gctgtcaaca tgctccccga tccggcattg cttcaggctc
     2161 ctgcttcgtt cttggctttc caacctgcgc ggtcatgtcg ttccggcccg agcggtacgt
     2221 ccgctacttt cagcgccctg taaccaccgg gccaccaccg gcccgccaag ctcattcgca
     2281 tgaagaaaac ccttacactc ctggggctca tccccgcatt ggccagcgct caatcactgg
     2341 tgaacaccac gccggagaac cggaccgtcc tgctggagga cttcaccggc atccattgtg
     2401 gctactgccc cgaaggccac gtgatcatgg ccgccttgga ggaacgcttc ggcccgcgtt
     2461 acgtgggtgt aggtgtccat gccggcggtt tcgccgtgcc tcaaggcaac gagcctgact
     2521 tccgcaccga cgaaggcacc gccatcgacg cgcacttcac catcagcggc taccccgccg
     2581 gcgtgatcaa ccgccacctc ttcgacggcg aggacgacct ggggcgcggc tcctgggaag
     2641 gtgccgtgga gcaggtgctc gacatgccca gcccggtgaa cctcggagtg gaaagctcct
     2701 acgatgccgg tacgcaaaca ctcaccgtgc acacgcacgc gctgtacacc ggcgacagcc
     2761 cctccggtaa cgactacatc agcgtgctgg tgaccgagag ccacatcatc ggctaccagg
     2821 ccgactacac caacggcacc caccagaact acgaccactt gcacgtgctt cgtggctacc
     2881 tcaccgacac ctggggcgag gatgtgggga accacgtggc gggggagacc gtggaccgca
     2941 cgtataccat ggccgtgccc ccggaatgga acatcgcgaa ttgcgaggtg gtcgccttca
     3001 tcagcgagta ccagaccgag gtgtaccagg cccgcgacgt ggcggccgac ggtggcacca
     3061 ccctggtgat cggcagcatg gccggcaatg gacagcccta tcgcccgggc caggatggta
     3121 cggccacacc cttcgatggc accttcacca acctgctgaa cgccgacgcg caatacacca
     3181 tcacactcac cggagatgat gcgcccggat cctggaccgc tggcatcaac gtggacgggc
     3241 aggacctcgg caaccccgcc actgttaccg tgaccaacgg ggccacgatc aacgtgacgg
     3301 ccaacatcac gcccgacgcc gcacccggca tcggggacta caccctctcg atcacgagcg
     3361 tttccaacag cagcgccccc gcgatctcga ccgaatacca tgtgatcagc ggtgtgcacg
     3421 acctgatcgt gacccactcg ggcgccgaag cgcatgagcc gatctacatg aacgccatgt
     3481 accacgaacc cgcgaaagcc gccacgtcca aagccaagtt cgtcgccttc gcccaggccg
     3541 acgcgctcgc cggggtgaac aacctgtacg tgaacgtgag ctggaccttc cccagcctga
     3601 ccgacgacga ggtggccgta ctcgcggcct tcatggacaa cggcggcaat ctgatgatcg
     3661 ccggccagga catcggctgg gaccagagcg gtgcggacgg cgcgtacggc acgcccgcca
     3721 cgcaggcctt ctacgccgac cacctgctgg ccgacttcat ggatgatggc agcacggccg
     3781 acaataccgt gaacttcgag gacgccgacg cggtcttcgg caccctttcg aactccgcgg
     3841 tgaacaacgt gttcagcacc aacacctacc cggaccgcat cgacccgatc gacccggcgg
     3901 tacccatcct tcggtacacc aacccggaca agatcggagg gctgcgtgcc gaggtgggca
     3961 accacaaagt ggtgtacttc ggcatcggcc ccgagcagat gagcaacacc gaagtgggcc
     4021 gcggcatggt gcaactgagc cacgactggt tctacgggat cgtcagcgtg gaggaattcg
     4081 acgcggccat ggccgacctg ggacgcccct acccctcgcc cgccaacgac gtggtgaacc
     4141 tcgatgtatc aagcatacat ggcgccacca ccctcgaagt gctcgatgcc agcggacgtc
     4201 tgatgctcac ccaggccgtg gcgaacaatg cgggtctggc caccctgcat gtgggctcac
     4261 tggccaacgg cgtgtacagc gtgcgtgccc ggggcccaca aggcaccggt gccgcccgcg
     4321 cgttcaacgt gctccactga acgctaccgc cgtgctccca cggaccccgg ccctgtgccg
     4381 gggttcgtgt tttacgaata tcccgttgga tcccacgcgc gggccggttg ttgcgatccg
     4441 cgcggagaac ccatgcatgc gacggggcat aacttgcccc gctcgaatga acgaaccata
     4501 cccgctccac acatgaccgg acgtctcctc ccgcttgtcg ccctccttac caccatttca
     4561 gtacacggcc agatcgcttt cggcgggcag ccgtacggca tgcgccccac cgaaaagacc
     4621 gggctgccac cggcgccgcg tgtgatcatg cccaccgtgg acgccgcggc gctgatggcc
     4681 gaggatgccg aacgcgcagc ccagggcatc aagggcccgt accgcttcgg cttcaaccac
     4741 gccaccgacc tgtccctcga caacagcggc atctggcaca ccatgcccaa cggcgaccgg
     4801 gtgtggcggc tggccatcgt atgccccaac gccttcagca tcaacttcga gttcaatgag
     4861 tacatggtgc ccgagggtgc gcgcgtgttc gtttacaatg cctggaacga ggtattgggc
     4921 ggattcaccg cggcgagcaa cggcgggcgc cccaccatgg gcgtgggcca gctggccggc
     4981 gaccgcatca cggtggagta cgtggagccc gcgtccgtcg aaggtcaggg ccatttgcgt
     5041 atcggccagg tgacccacgc ctaccgcgat gtgctcgggc tggccaaagg gctcggcgac
     5101 agcggcagtt gcaacaacaa cgtgatctgc cccgtgggcg acgaatggcg cgacgagatc
     5161 cgatcagtgg ccatcatcat cgtgggcggc agtggttatt gtaccggcac cctgttgaac
     5221 aactgcgcgg aggacggcac gccctacttc ctcaccgcca accactgcac cgagggggcc
     5281 aacgtgaaca actgggtctt ccgcttcaat tgggaaagtc ccgtgtgcga agccaaccag
     5341 aacgggccca ccaaccagac cgtcagcggc gcctcgcttc tggagaacag cggcggctcc
     5401 gatgtggctc tcctgcaact caacagcacc ccgcccagcg actacaacgt gtactacgcg
     5461 ggctgggaca acagcggcgc tgcccccacc tcggaggtgt gcatccacca tcccagcggc
     5521 gacatcaaga agatcagctt caacaatgat gcagcgggcg aggccgactg gggcagcgcc
     5581 gccacctggc
//
LOCUS       DAOQOC010000039         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_5857_length_5563_cov_3.117466, whole genome shotgun sequence.
ACCESSION   DAOQOC010000039 DAOQOC010000000
VERSION     DAOQOC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_5563_cov_3.117466"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PL106_01140"
     CDS             complement(<1..254)
                     /locus_tag="PL106_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HNA31699.1"
                     /translation="MIASILFVLLLGAASWLFARNIMRIRRNILLGRDVTINDRPGAR
                     WALMARVALGQSKMVVRPIAGFLHIIVYAGFVIVNIEVLE"
     gene            complement(251..1273)
                     /locus_tag="PL106_01145"
     CDS             complement(251..1273)
                     /locus_tag="PL106_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNA31700.1"
                     /translation="MKIKREYAIALMMIGAIALLWYGINYLKGFNLFGGRDVYYAKYN
                     DLAGISRASPVLYNGAKVGQVVSIRLMDDSAGTWLVSLQIDNKTLRVTKGSMAEIYSS
                     DFLTRAIRIVPGPGEVAHRGDTLRSGSEASITDALNQQIDPIKRKVEGMMVNLDSVVT
                     ALQAVFNVQAQQDIDSGLKSLRGTLENLERATGRLDALLASESEGIRSTLDNLNKLST
                     TLAGNSTELDRIFANADSITTALADGRLKKMLADLGEVSGQLKGTMARLNEGEGTLGK
                     LLKDDSLYVNLNKASKDLDLLLEDLRVNPNRYLSIFGKKDKLPKLSGADIERIQQAYQ
                     KQQGKP"
     gene            complement(1288..2445)
                     /locus_tag="PL106_01150"
     CDS             complement(1288..2445)
                     /locus_tag="PL106_01150"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNA31701.1"
                     /translation="MKARYRTLRWALFGAVVFGLPARTLPQVDPNVVDVVCLDAGHGG
                     KDPGNLGTGRFKTTEKHVALNVTKLVGKYIQENLPGVKVVYTREDDRFIELKERCNIA
                     NKAKADVFISIHCNANAKHEPHGAETYFMGLHKTEANMRTAMQENASILLEDGHDLKY
                     DGYDPKDPESMIALSLRQNVFMDQSAMLSAAVQEQFRDRAGRSDRGVKQAGFLVISYT
                     TMPAILIELGFLTNADEEDYMQSTKGQEHLASAIYRAFKSYKATIESKGAQVAVAPLP
                     ERPDTVTPEKEEKGADQPRDNGVRFKVQIVTSSKRIDLKPKNFNGLEGVQEMKGNDLW
                     KYTVGDERTLEDARALQRTCREKGFEGCFIVAFKEGARIDLQQAVTLARQP"
     gene            2621..5122
                     /locus_tag="PL106_01155"
     CDS             2621..5122
                     /locus_tag="PL106_01155"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNA31702.1"
                     /translation="MRTVFLTGLLTVGVGMSARAQSLQDKVTASARDSTRYDMAGQKL
                     YLFGAAKVAYEGLELTADRIVLDMKNETASAFGAPDSSGTIVGKPVFKQGGRTIEADS
                     LAYNFRTKEGLIREVRTTEDQLYALAHLSKRHANEEVHSKGGMLTTCDRPRPHYHFAV
                     SRMMVIPDDKIVTGPVIMKVGRVPLPLAAPFGLFPNHKNGAAGVLIPVFGYSDALGYY
                     LLNGGWYQPINDHVDAQLTGDIYSRGSWTLRALTRYRTRYRYSGNVDISRTTLLNSVP
                     EYPDFSRQTNFFVRWNHLMDPKASLTDRFSASVNFGSSGNFSNNFNSTQGDFLSNTFQ
                     SNIQWNHLWSGKPYNVAVGMRHSQNTLNKTYDITLPTVTFNVQRVFPADWFRGDAPTG
                     ERKWFDNIGLTWSSQFDNRISTTEDVLFSSGMDRVLRLMRNGLRHTAAINTSFKTRAF
                     SINPEVRLTDRMYFDQVRRTYDAEAGVLTTDTVARFGAPLDWSAGVSLTSKLYGMFTF
                     NKGRLKAIRHVITPTAGLSYTPGNDTRITGPFGTAGAEASYSPYEIGIYGTPSPSASG
                     LLTLGLVQSLEAKTLLPSGSGASQDSAQQTTRKIKLLDFLGATTSYDMLKDSVNWSPM
                     NVNARTRILDRVDVNVTSVWDPYATNEQGARINRSQRELDGRLARLSNANLALGFELK
                     SRRYGQSTTDTPVNNNTEKVVEEADPDKGARMNFNLPWHLRVNYSYDLARAWKQSDFT
                     DTQRQSVLFNGDVTILKWWKLGASSGYDLTAGEWTPTTINVYWDMHCWEFNVNVIPLG
                     TRKSIGFRINVKASVLRDLKYEWTRPFGNDGQLLR"
     gene            5150..>5563
                     /locus_tag="PL106_01160"
     CDS             5150..>5563
                     /locus_tag="PL106_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31703.1"
                     /translation="MLWRTLDSMNRTIKMIVGGALALVVLVWTVHDSFRQHQVIDTDD
                     WKTELRADAAGYYIYLPGVIHHGMRPGWLSDERLRIIGSGIQEDTARNIIRTKYTYGT
                     ALIELPFFLAAELIVGWGNSDGFTPVHHRAMDVAGV"
BASE COUNT         1060 a         1853 c         1579 g         1071 t
ORIGIN      
        1 atctccagca cttcgatgtt gacgatgacg aagcccgcat aaacgatgat gtgcaggaaa
       61 cccgcgatgg gccgcaccac catcttgctc tgcccgaggg ccacgcgcgc catcaatgcc
      121 cagcgcgcac ccgggcggtc gttgatggtc acatcgcgtc ccagcaggat attccgcctg
      181 atccgcatga tgttccttgc gaagagccag ctcgccgccc cgaggagcag aacgaagagg
      241 atgctggcga tcatggcttg ccctgctgct tctggtacgc ctgctggatc cgctcgatgt
      301 ccgccccgct gagcttgggc agcttgtcct tcttcccgaa gatactcaga taccggttcg
      361 ggttcacgcg aaggtcctcc agcagcaggt caaggtcctt gctggccttg ttcaggttga
      421 catataggct gtcgtccttc aacagtttgc ccaacgtgcc ttccccttcg ttcaacctgg
      481 ccatcgtacc tttcaactgg ccgctcactt cccccaggtc ggccagcatc ttcttcaacc
      541 gcccgtcggc cagcgcggtg gtgatgctgt ccgcattggc gaagatccgg tccagttcgg
      601 tgctgttgcc cgcgagcgtg gtgctcaact tgttcagatt gtccaaggtg gaccggatgc
      661 cttccgattc gctggccagc aacgcatcga gccggccggt ggcacgctcc aggttctcaa
      721 gggtgccccg caggctcttg agccccgagt cgatgtcctg ctgtgcctgc acgttgaaca
      781 cggcctgcaa ggcggtgacc acgctgtcca agttcaccat catgccttcc accttgcgtt
      841 tgatgggatc gatctgctgg ttgagcgcgt cggtgatgct ggcctccgat ccacttcgaa
      901 gggtatcgcc gcgatgggcc acctccccgg ggccgggcac gatccggatg gcccgggtga
      961 ggaaatcgct gctgtagatc tcggccatgg agcctttggt cacccgcagg gtcttgttgt
     1021 cgatctgcag ggacaccagc catgtgcccg cgctgtcgtc catcaaccgg atgctcacca
     1081 cctggcccac cttggcaccg ttgtaaagca ccgggctggc tcttgaaata cccgccagat
     1141 cattgtattt cgcgtagtac acatccctgc ccccgaacag gttgaagccc ttgaggtagt
     1201 tgatgccgta ccagagcagc gcgatggccc cgatcatcat cagggcgatg gcgtattcgc
     1261 gtttgatctt caccacgggc ttcggtccta cggctgacgg gccaatgtta cggcttgctg
     1321 cagatcgatc cgtgcgccct ccttgaaggc cacgatgaaa cagccctcaa agcccttttc
     1381 gcggcaggtc cgctgcaggg cccgtgcgtc ctccagtgtg cgctcatcac ccacggtgta
     1441 tttccacagg tcgttgccct tcatctcctg cacgccttcc agcccgttga aattcttcgg
     1501 cttcagatcg atgcgcttgc tgctggtgac gatctgcacc ttgaagcgca ccccattgtc
     1561 cctgggctgg tccgcaccct tttcctcctt ctccggcgtc accgtgtcgg gtctttccgg
     1621 tagaggtgcc acagccacct gcgccccctt gctctcgatg gtcgccttgt acgacttgaa
     1681 ggcacggtag atggcactgg ccaggtgctc ctgccctttc gtgctctgca tatagtcctc
     1741 ctcgtcagcg ttggtgagga agcccagttc gatcaggatc gccggcatgg tggtgtaact
     1801 gatcaccagg aaaccggcct gtttcacgcc ccggtcactc cgacccgcac gatcacggaa
     1861 ctgctcctgt accgcagcac tgagcatggc gctctgatcc atgaagacgt tctgcctcaa
     1921 actcagcgcg atcatgctct ccgggtcctt cggatcgtag ccgtcgtact tcagatcgtg
     1981 accatcctcc agcaatatgg acgcgttctc ctgcatggcc gtccgcatgt tggcttcggt
     2041 cttgtgcaac cccatgaagt aggtctccgc gccgtgcggc tcgtgcttgg cgttggcgtt
     2101 gcagtggatg ctgatgaaca cgtcggcctt ggccttgttg gcgatgttgc agcgttcctt
     2161 cagttcgatg aagcggtcgt cctcccgcgt gtacaccacc ttcacaccgg ggaggttctc
     2221 ctggatgtac ttgcccacca gtttggtgac gttgagcgcc acgtgcttct cggtggtctt
     2281 gaagcggccg gtgcccaggt tgccgggatc cttgccgccg tggcccgcat ccaggcatac
     2341 cacatccacc acgttcggat ccacctgtgg caaggttcgt gcggggagac cgaataccac
     2401 cgcaccgaag agcgcccaac gaagcgtacg atagcgagcc ttcatccgtt ctgcaccgat
     2461 gcgctggttc ccttggtcct gccgcgaacc ggctccccga tcatccccgg acgccggtcg
     2521 aaggacgcgg ggtactttcg tccgcccgat ggaccaaccc agtaaccctg ccgcgccatt
     2581 gcgcgaaagt agacgggtac ggtccggggc ttggtgggcg ttgcgcaccg ttttcctcac
     2641 gggcttgttg acagtgggcg tgggcatgtc cgctcgtgcg cagtcgttac aggacaaggt
     2701 gacggcctcg gcccgcgaca gtacgcgtta cgacatggcc gggcaaaagc tgtacctctt
     2761 cggggccgcc aaggtcgcct acgaggggct ggaactcacg gccgaccgca tcgttctgga
     2821 catgaagaac gagaccgcca gtgcatttgg tgcccccgac agcagcggga ccatcgttgg
     2881 gaagcccgtg ttcaaacaag gtggaagaac gatcgaggcc gacagcctgg cctataactt
     2941 ccgtacgaaa gagggtctga tccgcgaggt gcgcaccacc gaggaccagt tgtacgccct
     3001 ggcccacctg agcaagcgcc acgccaacga ggaggtacac agcaagggcg gcatgctcac
     3061 cacctgcgac cggccgcggc cccactacca cttcgccgtg agccgcatga tggtgatccc
     3121 ggacgacaag atcgttaccg ggcccgtgat catgaaggtg ggcagggtgc ccctgcccct
     3181 ggccgccccc ttcgggctct tccccaacca caagaacggg gccgcgggcg tgctcatccc
     3241 cgttttcggg tacagcgatg cgctcggcta ctacttgctc aacggcggtt ggtaccagcc
     3301 gatcaacgac cacgtggacg cccagctcac cggcgacatc tacagccggg gcagctggac
     3361 cttgcgcgcg ctcacccgct accgcacacg ataccggtac agcggtaatg tcgacatcag
     3421 ccgcaccacc ctgctgaaca gtgtgcccga ataccccgac ttcagccgcc agaccaactt
     3481 cttcgtgcgc tggaaccacc tgatggaccc caaggccagc ctcaccgacc gcttcagcgc
     3541 cagcgtcaac ttcggcagca gcggcaactt cagcaacaac ttcaacagca cccagggcga
     3601 cttcctctcc aacacattcc agagcaatat ccagtggaac cacctatgga gcggcaagcc
     3661 gtacaacgtg gccgtcggca tgcgccacag ccagaacacc ctgaacaaga cctacgacat
     3721 caccctgccc acggtcacct tcaacgtgca gcgcgtcttc ccggccgact ggttccgggg
     3781 tgatgctccg accggcgagc ggaagtggtt cgacaacatc ggcctcacct ggagttcgca
     3841 gttcgacaac cgcatcagca ccaccgagga cgtgctcttt tccagcggca tggaccgcgt
     3901 tctacgactc atgcgcaacg gcctgcgcca caccgccgcc atcaacacct ccttcaaaac
     3961 gcgcgccttt tccatcaacc ccgaggtgcg cctcacggac cgcatgtact tcgaccaggt
     4021 acgcaggacc tacgatgcgg aggcgggtgt gctcactacc gacaccgtgg cccgcttcgg
     4081 tgcgccactg gactggagcg cgggcgtctc gctcaccagt aaactgtatg gcatgttcac
     4141 cttcaacaag ggacgactga aggccatccg ccatgtgatc actcccaccg ccgggttgag
     4201 ctacacgccc ggtaacgata cccgcatcac cggacctttc ggcaccgctg gcgccgaggc
     4261 cagctattcg ccgtacgaga tcggcatcta cggcacgccc tcgcccagcg ccagcgggct
     4321 gctcaccctc ggactggtgc agagcctgga ggccaaaacg ctcctgccca gcggatcggg
     4381 agcttcgcag gacagcgcac agcagaccac ccgcaagatc aaactgctcg atttcctcgg
     4441 ggccaccacc agctacgaca tgctgaagga ctcggtgaac tggagtccga tgaacgtgaa
     4501 cgcccgaaca cggatcctgg accgtgtgga tgtgaacgtg accagcgtgt gggatccgta
     4561 cgcgacgaac gaacagggtg cccgcatcaa ccggtcgcag cgcgaactgg acggccgcct
     4621 ggcgcgcctc tccaatgcca acctcgccct gggcttcgaa ttgaagagcc gtcgctatgg
     4681 ccagagcacc accgacacac cggtgaacaa caataccgaa aaggtagtgg aagaagcgga
     4741 cccggacaag ggtgcccgca tgaacttcaa cctgccctgg cacctgcgcg tcaactacag
     4801 ctacgacctg gcacgtgcgt ggaagcagag cgacttcacc gacacacaac gccagagcgt
     4861 actcttcaac ggcgatgtga ccatcctgaa atggtggaaa ctcggtgcca gcagcggcta
     4921 cgacctcacc gccggtgaat ggaccccgac caccatcaac gtgtactggg acatgcattg
     4981 ctgggagttc aatgtgaacg tgatccccct gggcacccgc aagagcatcg gtttccgcat
     5041 caatgtgaaa gccagcgtgc tacgcgatct gaagtacgaa tggaccagac ccttcgggaa
     5101 tgacgggcaa ctcttacggt agcctgcagt atcgttgaac cgaccaccca tgctctggcg
     5161 aacgctcgac tcaatgaaca ggacgatcaa gatgatcgtc ggcggcgcat tggcactggt
     5221 cgttctcgtt tggaccgtgc atgactcctt ccggcagcac caggtgattg ataccgatga
     5281 ctggaagacc gaactgcgtg cggacgcagc cggatactac atctaccttc ctggcgtcat
     5341 ccatcacggc atgcgcccgg ggtggctttc ggacgagcgg ctgcgcatca tcggcagcgg
     5401 gatccaggag gataccgcac ggaacatcat ccgcacgaaa tacacctacg gcaccgcact
     5461 tatcgaactc cccttcttcc tggccgccga actgatcgtg ggctggggga actccgatgg
     5521 tttcacgccc gtgcaccatc gcgccatgga cgtggcgggg gtc
//
LOCUS       DAOQOC010000040         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6066_length_5435_cov_3.026022, whole genome shotgun sequence.
ACCESSION   DAOQOC010000040 DAOQOC010000000
VERSION     DAOQOC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6066_length_5435_cov_3.026022"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PL106_01165"
     CDS             complement(<1..1376)
                     /locus_tag="PL106_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31704.1"
                     /translation="MQHMPKHMLATIALILLSAQLNAQCLNTSQYPANAITPLTGGQT
                     TTIHTCCWQTEFAVVTGIIPSASYTFTYGGGGWVTVREGAYDGPVLGQGDSPLTVEAT
                     TAQDLFAHWNTDSLCNTAQVCHASTVLMSNPGCDPPVASTSIINDCGAQQFSVEVEVQ
                     SLGDAPSVGLNWTVNNGAPSNLGGLQTGTYSIGPFPFGAIVDLVVMHAGNPGCNVAMN
                     DLTNGGCAIVGCGPETYTYCYPNNAYLIQTFQGDQGYPLRITFNSGMVTNTGNDPLTI
                     HDGFTPQDPVLFSGIGNNGDLTGVTVTSTNPDHALTIRFTSNSSFSCTDGGLTPEWNY
                     TVGCLDCEPASATVGEVETDCGAQGFSVLVNVTDMGSAEALQITNDAGLPPTDVNGTG
                     VYTAGPFPIGTPVALVLENGENTLCSVSLGSFINTMCATIVDCEEPPLEQTYCYGEYD
                     TEEWLYQSS"
     gene            complement(1466..3460)
                     /locus_tag="PL106_01170"
     CDS             complement(1466..3460)
                     /locus_tag="PL106_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31705.1"
                     /translation="MQRAILLITVVCCMACVLHAQEVLVPLNNGWRFQQVGKDRWYDA
                     EVPGVVQTDLLRHGLIPDFYRGSNIDSVQWIENEDWEYRRTILVADTLLRHGHLDLVF
                     KGLDTFAEVYLNDSLVGRANNMFRTWEWEVKDLLREGPNELRVIFRSPIREGAKLRDA
                     YGIQLPHDNDPSGVSPYIRKAAYQFGWDFCPRLVTSGIWQGVELRGWSVGRIRQVVPT
                     QFDHGNTVHLKVVVDSDGAVGELDSKCGTTVQLWEPDSERPAVLNCSGHLDGVTFRIK
                     RPKRWDPRGSGEQPLYRVRTRLTCDDTVRSVHEMRIGIRTVEFDTTRDADGQAFAFKV
                     NGKNVFMKGCNIVPPHMVPSLDADRKWVELVKHMCEAGMNMVRVWAGGIYPPDAFFDA
                     CDTAGILVWQDHMYAQLPPGSKSFMENAEQEAFQQQHRLLRHPSLAIWCGNNELDVAW
                     KNWGWQERYHLHGADSISVANASTELFNARNGYLSPFFRSPLYTPTSPLSNWGNAEGL
                     TRGDLHYWGVWHGDSAFSSFANNVGRFVSEYGFQSYPDSALLARYNPPSELYLGSPTM
                     KRLQRSYKTDKPIIDAIKRELGVEPRTLGAFIRASQEVQARAYRMAIEAHLGARPRCM
                     GTLFWQLNDCWPGPSWSLIDYEGHWKPGMYAVQRAYQQEH"
     gene            complement(3532..4284)
                     /locus_tag="PL106_01175"
     CDS             complement(3532..4284)
                     /locus_tag="PL106_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNA31706.1"
                     /translation="MRVRVEVCITSVDEAMAAMNAGADSVEVCTWLAAGGLTPGPGLV
                     SLVRERSKGTGTWSRVLVRPSPGGFRYTTDERQIILRDALLLAVADDTAGIVAGALRA
                     DGQADAELMDLVRATLNGRELTFHRAIDHAADMPAALEQCIGFGAARVLTSGGATRAL
                     DGAAMLKRLVEGAGERITIAAGAGINPGNVVELVERTGVREVHFSAQRPALLEHAGVA
                     MSAEHAGVNFAVEPDTAKIEGVMNALVKAGLR"
     gene            4453..4526
                     /locus_tag="PL106_01180"
     tRNA            4453..4526
                     /locus_tag="PL106_01180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4487..4489,aa:Ala,seq:cgc)
     gene            4597..>5435
                     /locus_tag="PL106_01185"
     CDS             4597..>5435
                     /locus_tag="PL106_01185"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HNA31707.1"
                     /translation="MTVLLLCAALGCDQTPPIAVDTTSPAVELVNPFIGTGGHGHTFP
                     GACVPNGLVQLSPDTRPEGYLDWDGCSGYHNSDSLIYGFSHTHLSGTGVPDLCDVLLM
                     PMVDKLDLDPAKYRSPFRKSTEHAHAGYYTVQLDGPEVRAELTATQRTGVHRYTFPPE
                     VSARLVLDLVHRDKLIAGYAEPINAREIVGERRSSSWARDQHLFFCIRFSHPMRSGEH
                     GFFWNSLSNDTVAPLRRAQKAVVNFGKLDAPLVVTVGISAVSIEGARKNLEAEVPDQD
                     FDAV"
BASE COUNT         1007 a         1798 c         1593 g         1037 t
ORIGIN      
        1 gaactctggt aaagccactc ttcggtatcg tactcgccgt aacaataggt ctgttccaat
       61 ggcggctcct cgcaatccac gatcgtggcg cacatggtgt tgatgaagga tcccagggat
      121 acggaacaaa gggtattctc cccgttctca agcacgagtg ccacaggcgt tcctatgggg
      181 aaaggtccgg ccgtgtacac acccgttccg ttcacgtccg tgggtggcaa acccgcatca
      241 ttcgtgatct gcagcgcttc ggcggaaccc atgtcggtca cgttcacaag caccgagaat
      301 ccctgcgctc cacaatcggt ctccacctcc cccactgttg cggaggccgg ttcacagtcc
      361 aggcagccca cggtatagtt ccattccggg gtcaacccgc catcggtgca actgaaactg
      421 ctgttcgatg tgaaacggat ggtgagcgcg tggtcgggat tggtggacgt tacggtgacc
      481 ccggtgaggt ccccgttgtt gccgatgccc gagaagagca ctggatcctg cggtgtgaag
      541 ccgtcgtgga tggtgagcgg atcattgccc gtgttggtca ccatgccgct gttgaaggtg
      601 atccgcaacg gatagccctg atcgccttgg aaggtctgta tgagatacgc gttgttcggg
      661 tagcagtagg tgtaggtctc ggggccgcag cccacgatcg cgcatccacc attggtcagg
      721 tcgttcatcg ccacgttgca gcccggattc cccgcgtgca tcaccaccag atcaacgatc
      781 gcaccgaacg ggaaaggccc gatgctgtac gtgccggtct gcaagccccc gaggttgctg
      841 ggggcgccat tgttcaccgt ccagttcaat ccgacggaag gcgcatcgcc caggctctgc
      901 acctccacct ccacactgaa ctgctgggcc ccgcaatcgt tgatgatgct ggtggaggcc
      961 acagggggat cgcaacccgg gttcgacatc aggaccgttg aagcatggca tacctgcgcg
     1021 gtgttgcaca ggctgtcggt gttccaatgg gcgaaaagat cctgtgcggt ggtcgcctcc
     1081 acggtcaggg gcgagtcccc ctgccccaac accggtccat cgtacgcgcc ttcgcgcacg
     1141 gtcacccaac caccacctcc gtaagtaaag gtgtacgagg cactcgggat gatcccggtc
     1201 accaccgcga actccgtttg ccagcaacag gtatggatgg tggttgtttg tcctccggtg
     1261 agcggcgtga tggcgttggc ggggtattgt gatgtgttga ggcattgtgc gttcaactgt
     1321 gcggagagca ggatgagcgc gatcgtcgcc agcatgtgct ttggcatgtg ttgcatgggc
     1381 ttgtagatgg tcgtgggggt cggtgaatgg actccgggag gaagggccaa ggtgcccgaa
     1441 tatcggtgga taagcgatcg gtggctcagt gttcctgctg atacgcccgc tggaccgcgt
     1501 acatcccggg tttccaatgg ccttcgtagt cgatgaggct ccagctcgga ccgggccagc
     1561 aatcgttcaa ctgccagaag agcgtaccca tgcaacgcgg tcgcgcaccc aggtgcgcct
     1621 caatggccat gcggtacgcc cgggcctgca cttcctggct ggcccggatg aacgcaccca
     1681 gggtcctcgg ctccacgccc aactcccgct tgatcgcgtc aatgatcggc ttgtcggtct
     1741 tgtaactgcg ctgcagacgc ttcatcgttg gcgatcccag gtataattct gatggggggt
     1801 tgtaccgggc gagcaaagcg ctgtccgggt agctctggaa tccgtactca ctcacgaagc
     1861 gccccacgtt gttcgcgaac gaggagaagg cactgtcgcc gtgccacacg ccccaatagt
     1921 gcaggtcgcc ccgggtgagg ccctcggcat tgccccaatt gctcagcggt gaggtgggtg
     1981 tgtacaaggg ggatcggaag aacggcgaca ggtatccgtt ccgggcattg aacagttccg
     2041 tggatgcgtt cgccacgctg atcgaatcgg ccccatgcag atggtagcgc tcctgccacc
     2101 cccagttctt ccacgccacg tcgagttcgt tgttgccaca ccagatggcg agcgacgggt
     2161 gcctcagcag gcgatgctgc tgttggaacg cttcctgttc agcattctcc atgaaggact
     2221 tcgaacctgg cggaagttgg gcgtacatat ggtcctgcca taccaggatc cccgccgtgt
     2281 cgcacgcatc gaagaacgca tccggtggat agatgccacc ggcccatacg cgcaccatgt
     2341 tcatcccggc ctcacacatg tgcttgacca gttccaccca cttcctgtcc gcgtccagcg
     2401 aaggcaccat gtgcggtgga acgatgttgc aacccttcat gaacacgttc ttgccattca
     2461 ccttgaacgc gaaagcctga ccatcggcgt cccgcgtggt gtcgaactcc acggtacgga
     2521 taccgatgcg catttcatgt acgctgcgga ccgtatcgtc gcacgtcaaa cgggtgcgca
     2581 cccggtacag gggttgttcg ccagaaccac gcggatccca tcgcttgggc cgcttgatcc
     2641 tgaaggtgac cccatccagg tggccggaac aattgagcac cgcaggccgc tcgctgtccg
     2701 gttcccacag ttgcaccgtg gttccgcact tggagtccaa ctcaccgacc gctccatcgc
     2761 tatcaaccac caccttcagg tgtaccgtat tcccatgatc gaactgggtg ggaaccacct
     2821 gcctgatcct cccgacactc cacccccgca actccacccc ctgccagatc ccgctcgtga
     2881 ccagcctcgg gcagaaatcc cagccgaatt gatacgccgc cttccggatg tacgggctca
     2941 ccccgctggg atcgttgtcg tggggcaact ggatcccgta cgcatcgcgc agcttcgctc
     3001 cttctctgat cgggctgcgg aagatcaccc tcaactcgtt ggggccctca cgcagcagat
     3061 ccttcacctc ccattcccac gtacggaaca tgttgttcgc ccggcccacc agggagtcgt
     3121 tcaggtagac ctcggcgaag gtgtcgagcc ctttgaacac cagatcgagg tgcccgtgac
     3181 gcagcagggt gtccgcaacg aggatcgtcc gccgatactc ccaatcctcg ttctcgatcc
     3241 actgcacact gtcgatgttg ctcccccggt agaagtcggg gatcagtcca tggcgcagca
     3301 ggtcggtctg caccacaccg ggcacctccg catcatacca ccggtccttc cccacctgct
     3361 ggaatctcca cccgttgttc aacgggacca ggacctcctg ggcgtggagg acgcaggcca
     3421 tgcaacatac gaccgtgatc agcaggatcg ctcgttgcat gcgtgccgaa aggggccagg
     3481 atgcacggtg cccgcgcacc ggctgatccg tacaccggag cctcgtggta ctcatcgcag
     3541 acccgctttc accaacgcat tcatcacccc ttcgatcttc gccgtatcgg gctccaccgc
     3601 gaagttcaca ccggcatgtt cggcactcat ggccacaccg gcatgctcca gcagtgcagg
     3661 ccgttgggcg gaaaaatgta cctcccgcac accggtgcgt tccaccagtt ccaccacgtt
     3721 gcccgggttg atgcccgcgc ccgcggcaat ggtgatccgc tcccccgccc cttccaccag
     3781 gcgcttcagc atcgccgctc cgtccagcgc acgcgttgcg ccacccgagg tgagcacacg
     3841 tgccgcaccg aagccgatgc attgctccaa ggcggcgggc atgtccgcgg catgatcgat
     3901 cgcgcggtga aaggtgagtt cgcgtccgtt gagcgtagcg cgtacgaggt ccatcaattc
     3961 cgcatcggcc tggccatcgg cacgcaaggc ccctgcaaca atgcccgcgg tatcgtcggc
     4021 taccgcgagc agcaaggcat cccgaaggat gatctgccgc tcgtcggtgg tataccggaa
     4081 gccgccgggg gaaggccgca ccagcactcg gctccacgta ccggtaccct tcgatcgctc
     4141 acggaccaaa ctcacgagtc cgggtccggg cgtgagcccg ccagcagcca gccaggtgca
     4201 gacctcaaca ctatcggcac cggcgttcat ggccgccatt gcttcatcca ccgaggtgat
     4261 acaaacctcc acccgtacgc gcatgggggc aaggtagctg tcgcgatcgc gaacccctcg
     4321 gacagggtat ggctatccgt tcttgaagcc ggggctccgg aaccggccga tcgcccttac
     4381 caaaaaggtc cgtccaacgg ttcggccgcg tgcgccatat ttgcaccccg ttcgcgggca
     4441 tgttgacatc ctggggcgat agctcagctg gccagagcgc cgcgttcgca atgcggaggt
     4501 cgagggttcg actccctttc gctccacccc gaagaagccc cggcgaaccg gggctttctt
     4561 tcttcagtcc ctgatcagcg caccgatccc ttcgccatga ccgtcctcct cctctgcgcc
     4621 gccctcgggt gcgatcaaac gccgcccatc gccgtggata cgacctctcc ggcggtcgag
     4681 ctcgtcaacc cgttcatcgg caccggcgga catggccaca cgtttccggg agcttgcgtg
     4741 ccgaacggcc tggtgcagct gagcccggac acccggcccg aaggatacct cgactgggac
     4801 ggctgcagtg gataccacaa tagtgatagc cttatttacg ggttcagcca tacgcacctg
     4861 agcggcaccg gcgtgcccga cctgtgcgac gtgctgctga tgcccatggt ggacaagctc
     4921 gacctcgacc ccgccaagta ccgatccccg ttccggaaga gcaccgaaca cgcccatgcc
     4981 gggtactaca ccgtgcaact ggacggaccc gaggtacgcg ccgaactgac cgcgacccaa
     5041 cgcaccggcg tacaccgcta caccttcccg cccgaggttt ccgcacgcct ggtgctggac
     5101 ctcgtgcatc gcgacaagct catcgcgggc tacgccgaac cgatcaacgc ccgggagatc
     5161 gtgggcgagc gccgaagttc ctcgtgggca cgcgaccaac acctgttctt ctgcatccgc
     5221 ttcagtcacc ccatgcgcag cggtgagcac ggcttcttct ggaacagcct ctccaacgac
     5281 accgtggccc cgttgcgccg tgcgcagaaa gccgtggtga acttcgggaa gctggacgca
     5341 ccgctcgtgg tgacggtggg tatcagcgcc gtgagcatcg aaggggccag gaagaacctg
     5401 gaggccgaag tccccgacca ggattttgat gcggt
//
LOCUS       DAOQOC010000041         5424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6087_length_5424_cov_3.360589, whole genome shotgun sequence.
ACCESSION   DAOQOC010000041 DAOQOC010000000
VERSION     DAOQOC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6087_length_5424_cov_3.360589"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PL106_01190"
     CDS             <1..187
                     /locus_tag="PL106_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31708.1"
                     /translation="WTSGVDVDHARIMDLMGRTIAEARPMGLRQGQMDLSRMPSGHYV
                     LQLSGSGRISRTIVVHE"
     gene            180..1883
                     /locus_tag="PL106_01195"
     CDS             180..1883
                     /locus_tag="PL106_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA31709.1"
                     /translation="MSDHAIPFGRKVRGYGLALVLGVSPWFPALAQPLNPLLIPPTVD
                     EDTIDLLIDQHVVQFYPGLHTGTYGVNAPYLGPTLILHHGDTAHLRVVNLLDTEMTSL
                     HWHGLQLPGEMDGGPPREVMPGDTWNVKFKVKNPAGTFWYHPHPHGHTAEQVQFGIAG
                     MIILRDSAEAALDLPRTYGVDDIPLVLQDRRFDENGQFVFGPYGDSVLVNGTPHPYME
                     LPAQVVRLRLLNGSNARVYQVGFDDDRSFSIIGNDGGLLEEPIPVDRVRLSNGERSEV
                     LVDLSGMEGDSLMLMGFGSELTETMPGGSLILNEPSYLNAIDFPLLRIRVGPPTDDPV
                     TSFPSSLVTLTPPDMADVTRTRHKAIDGFGTAGTGLFTFDGDTFNMDVVNDTVPLGTT
                     EIWYVLNATDIAHPFHIHGGSFFLLDRDGQQPHPWEQGWKDVVLVDMGEQVRFIMRFD
                     ERTDGWPFMYHCHNLMHEDLMLMLQYITEDPSTNVAAVPTTSEVRVFPSPTVDRLQFR
                     SGSPVDRVVVQDVLGREVMAANMRGASQGSLDVATLPSGMYHVLLRSHGVVLRGSFVK
                     E"
     gene            complement(1957..2373)
                     /locus_tag="PL106_01200"
     CDS             complement(1957..2373)
                     /locus_tag="PL106_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31710.1"
                     /translation="MKKITIITILAVALASCTKDEGPLYVPEPASAGDPVDTAYFNAE
                     VLPIFTAHCWTCHPPMGDMDLSAAEAYNSLVGVESTNHAPALRVAPGDPEGSVLWNKI
                     NYTEVYGLGMPPDGTALSSEELATIRDWIEQGALNN"
     gene            complement(2378..3286)
                     /locus_tag="PL106_01205"
     CDS             complement(2378..3286)
                     /locus_tag="PL106_01205"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNA31711.1"
                     /translation="MMNRILLFTFIAVTTALSSHAQEGAAREYVTGTFAGTQVVNAPS
                     VEVVPRKRSFGFMIQHRFGAISPDEQAWKQFAGLDLPANIRFAFQYAPFRDVQLEIGR
                     SKSGKVWDLGLKARVLKQSVEEEMPLSLTVLANAALMSDDFPAVSDRDFYADGERPFN
                     YRFEHRFSYNTQVIIGRRFAPWFSAQVAPVVIYRNLVPIGGSNLTLAVTGAARFKVST
                     KGSILMEATPIIQGGPPGEHRTPAAIAYELATSGHVFQIVLASGQEIVEQRLYTSPVA
                     RYEEGYMHLGFNIARTLFVKPKGPKP"
     gene            complement(3289..3675)
                     /locus_tag="PL106_01210"
     CDS             complement(3289..3675)
                     /locus_tag="PL106_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNA31712.1"
                     /translation="MMRALIILAAVLALAMAIPGDPWKAPAEADKLKDPMASNPKAAT
                     MGAKVFQSLCWTCHGLEGRGDGPNASALKVRPADLGTAMVQGQSNGALYWKITHGRGE
                     MASYEQVLSREDRWALTHHIRTLAHP"
     gene            complement(3672..>5424)
                     /locus_tag="PL106_01215"
     CDS             complement(3672..>5424)
                     /locus_tag="PL106_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA31713.1"
                     /translation="VGTLVFGRFFCSWGCHILALQDAAGWIMDKLRIKRQPIRSRTLI
                     WVPMAVMFYLFIWPQIMQLWHGVEQQAVHVVEAGGSRWSSFTTEDMWRNLPPPGVAVL
                     TFFVCGFVIVYLLGSRGFCFQACPYGALFGLADQLAPGRIVLTKDCDQCGLCTKACTS
                     DILVHRELAMHGMVTNPRCLKDLDCVAACPEKAVNFGFRIPPLFRKGHPMGAYGGRFS
                     NSMGEDVFLAIAFLIAMPVYRGLYDRVPFLLAVALALCTAWLFVMGWRLARKGAMQFR
                     DLTLKMDRRIRPAGRVFAVAITIVGLFFAHSAWVQLHTFLGTRGFAQVMAQRGDAEDL
                     NATIGHYESALRIGLIAPIDRRFELASLYQQRGETQRSMELLGDILRDDPRHSAARFR
                     MGELSMAQGDRDAAMRAWEEGLLQGEKRSGTRDHEMLARSALALGAAKEAMKDAEGAR
                     NAYAMGLDRVPDNAALLLASGTLAHRTGHPEKAIRFYEEALHHGGPEAQLHNNIGALY
                     LQQGRLEQALPHLERVAALKPSDAHVRYTLGVVFARLGRMDKAMVALRTSVQLDPSDQ
                     RTQQALAQVQQLNTASQ"
BASE COUNT         1013 a         1668 c         1694 g         1049 t
ORIGIN      
        1 ctggacgagc ggggttgatg ttgatcacgc acggatcatg gacctcatgg ggcggacgat
       61 agccgaggcc cggccgatgg gcctgcgaca ggggcagatg gacctgtcga ggatgccaag
      121 tgggcattat gtgcttcaac tgagcggatc gggccgcatt tcgcgtacta tcgtggtgca
      181 tgagtgacca cgcgatcccg ttcggccgga aggtgcgggg ctatggactc gccctcgtcc
      241 ttggcgtgtc cccatggttc ccggccctgg cccaacccct gaatccgctg ctcattccac
      301 cgacggtgga tgaggatacc atcgacctgc tcatcgacca gcatgtcgtc caattctatc
      361 ccggactcca taccggcacc tatggtgtca acgcgccgta cctgggcccg accctgatcc
      421 tccaccatgg cgatacggca cacttgcgcg tggtgaacct gctggatacg gagatgacca
      481 gcctgcactg gcacggcttg cagctgcccg gtgaaatgga cggtggtcca ccacgcgagg
      541 tgatgcccgg ggatacgtgg aacgtgaagt tcaaggtgaa gaacccggca ggtaccttct
      601 ggtaccaccc gcacccccac ggccatactg cggaacaggt gcaattcggt attgccggca
      661 tgatcatcct gcgggacagt gcggaggccg cgctcgatct gccacgcacc tacggcgtgg
      721 atgatatccc gctggtgctg caggaccggc gtttcgatga gaatggccag ttcgtcttcg
      781 gcccgtacgg cgattctgtc ctggtgaacg gtacgccaca cccgtacatg gaacttcccg
      841 cgcaagtggt gcgcctccgc ctgttgaacg gttccaatgc gcgtgtctat caggtcgggt
      901 tcgatgatga tcgctcgttc agtatcatcg gcaacgatgg tggccttttg gaggagccca
      961 tcccggtgga ccgtgtgcgc ctgagcaatg gcgaacgctc cgaggtgctc gtggacctgt
     1021 cgggcatgga aggggacagt ctgatgctca tgggtttcgg ttccgaactc acagagacca
     1081 tgcccggggg ttcgctcatc ctcaacgagc ccagttacct gaacgcgatc gacttccccc
     1141 tgttgcgcat ccgcgtgggg ccacctaccg atgatccggt aacgtcattc ccgtcctcgc
     1201 tggtcacctt gaccccgccg gacatggctg atgtgacccg tacacggcac aaggcgatcg
     1261 acggcttcgg cacagcgggt accggtctgt tcaccttcga tggcgacacc ttcaacatgg
     1321 atgtggtgaa cgataccgta cctctgggaa ccaccgagat ctggtacgtg ctcaacgcca
     1381 ccgacatcgc ccaccccttc catatccatg ggggatcctt cttcctgctc gatcgcgatg
     1441 ggcagcaacc tcatccgtgg gagcaggggt ggaaggacgt ggtgctggtg gacatgggcg
     1501 agcaggtgcg cttcatcatg cgcttcgatg aacgcacgga cggatggccg ttcatgtacc
     1561 actgccacaa cctgatgcac gaggacctga tgctgatgct gcaatacatc acggaagacc
     1621 cgagcaccaa cgtggctgcg gtaccgacga ccagtgaagt gcgggtcttc ccatcaccga
     1681 ccgtggacag gttgcagttc cgcagcggat cccccgtgga ccgggtggtc gttcaggatg
     1741 tgctcggtcg cgaggtgatg gccgcgaata tgcgaggcgc ctcccaaggg tcccttgacg
     1801 tggcaactct tccttcgggc atgtatcacg tgttgttgag gtcgcatggt gtggtgctgc
     1861 ggggcagctt cgtgaaggag taagcgacgg tgatcacatg cgtatcacgc tgttcgcacg
     1921 gatatcgggg tcggcaccgt tagccgctgg accgcttcag ttgttcaacg ccccctgctc
     1981 gatccaatcg cggatggtgg ccagttcctc gctgctcagc gcggtgccgt cgggcggcat
     2041 gccgaggccg tacacctcgg tgtagttgat cttgttccaa agcacgctcc cttccgggtc
     2101 gccaggggcc acgcgcaacg cgggcgcgtg attcgtactc tccacgccca ccagactgtt
     2161 gtacgcttcg gccgctgaga ggtccatgtc gcccatgggc gggtggcagg tccagcagtg
     2221 tgcggtgaag atgggaagga cctcagcatt gaaataggcc gtgtccacgg gatcccccgc
     2281 ggaagcgggt tccggcacgt acaatggtcc ttcatccttg gtacaggatg ccaacgccac
     2341 tgccaggatc gtgatgatcg tgatcttttt catccgttca gggctttggt cccttcggtt
     2401 tcacgaaaag ggtacgtgcg atgttgaaac ccaggtgcat gtatccttct tcataccgtg
     2461 cgaccgggga tgtgtacagc cgctgctcca cgatctcctg gccgctggcg agcacgatct
     2521 ggaacacatg tcccgaggtc gccagttcgt aggcgatggc tgccggtgtc cgatgttctc
     2581 ctggcggccc accttggatg atgggagtcg cttccatcag gatggacccc ttggtgctga
     2641 ccttgaagcg cgccgctccg gtgaccgcga gtgtcaggtt ggagccgcca atgggtacca
     2701 gattgcggta gatgaccacc ggggccacct gtgcggagaa ccatggtgcg aatcgccgac
     2761 cgatgatcac ctgggtgttg taggaaaagc gatgctcgaa ccgatagttg aatggacgtt
     2821 cgccatcggc gtagaaatca cgatcgctca ccgccgggaa atcgtcgctc atcaacgcgg
     2881 cgttggccag cacggtaagc gagagcggca tctcttcctc cacgctctgt ttcagcacgc
     2941 gtgccttcag tccgaggtcc cacaccttgc cgcttttcga acggccgatc tccagttgca
     3001 catcgcggaa cggcgcgtac tggaacgcga atcggatgtt ggccgggagg tcgagccctg
     3061 cgaattgctt ccacgcctgc tcatcggggc tgatcgctcc gaagcgatgc tggatcatga
     3121 agccgaacga acgcttgcgc ggcaccacct ccacgctggg tgcattcacc acctgtgtgc
     3181 ccgcgaacgt gcccgtcacg tattcacgtg ccgcaccctc ctgcgcatgc gatgacaacg
     3241 cggttgtgac cgctatgaag gtgaacaaga ggatccggtt catcatactc atggatgcgc
     3301 aagtgttcgg atgtgatggg tgagtgccca acggtcctca cggctgagca cctgctcgta
     3361 cgaggccatt tcgccccggc catgtgtgat cttccaatag agcgcaccgt tgctttgtcc
     3421 ctgcaccatc gccgtaccca gatcggcggg ccgtaccttg agggcggaag cattcggacc
     3481 atcaccacgg ccttccaagc catggcaggt ccagcacaac gactggaaga ccttggcccc
     3541 catcgttgcg gccttggggt tggaggccat cggatccttg agcttgtccg cttccgccgg
     3601 cgccttccat ggatcaccag gtatcgccat ggccagggcg aggacagcag cgaggatgat
     3661 gagcgcgcgc atcattggga ggcggtgttg agttgctgga cttgtgccag cgcttgctgc
     3721 gtgcgctggt ccgaaggatc gagttgtacc gatgtgcgca aggcgaccat cgccttgtcc
     3781 attcggccca agcgcgcgaa caccacgccc agcgtatagc gtacgtgcgc atcggacggc
     3841 ttcagggccg cgacccgctc gaggtgcggc agcgcttgtt ccagcctgcc ctgttgcagg
     3901 tacagcgcac cgatgttgtt gtgcaactgc gcctcaggtc caccatggtg cagcgcctct
     3961 tcataaaacc ggatggcctt ttcggggtga ccggtacgat gcgccaaggt gcccgatgcg
     4021 agcagcagag cggcgttgtc cggcacgcga tccagaccca tggcataagc gttccgcgca
     4081 ccttcggcat ccttcatcgc ctccttcgcc gcgcccaagg ccaatgccga gcgcgcgagc
     4141 atctcgtggt cgcgtgtacc ggaacgcttc tcaccctgca acagaccctc ttcccacgca
     4201 cgcatggctg cgtcgcgatc gccttgtgcc atggacaatt cgcccatgcg gaatcgtgcg
     4261 gctgaatgcc ggggatcatc gcgcaggatg tcgccgagga gttccatgct ccgttgcgtt
     4321 tcgccgcgtt gctgatacag cgaagccagt tcgaatcgcc tgtcgatggg cgcgataaga
     4381 ccgatgcgca atgcggattc gtagtggccg atcgtcgcat tcaagtcctc cgcgtcccct
     4441 cgttgcgcca tcacctgcgc gaaaccacgt gtgccgagga aggtgtgcaa ctgcacccat
     4501 gcgctgtggg cgaagaacaa gcccacgatg gtgatcgcca cggcgaacac acggcctgcg
     4561 ggacggatcc ggcggtccat cttcaaggtg agatcacgga attgcatggc gcccttgcgc
     4621 gccaggcgcc agcccatgac gaagagccag gccgtgcaga gcgcgagcgc cacggccagg
     4681 aggaagggta cacgatcgta cagaccgcgg tacacgggca tggcgatgag gaaagcgatg
     4741 gccaggaaca catcctcgcc catcgagttg gagaagcgcc cgccatatgc gcccatcgga
     4801 tggcccttgc gaaaaagcgg cgggatgcgg aagccgaagt tgacggcctt ctccggacag
     4861 gccgctacgc agtccaggtc cttcaggcag cgcggattgg tgaccatgcc gtgcatggcc
     4921 agttcgcggt gaacgaggat gtccgatgtg caggccttgg tgcagaggcc gcactggtcg
     4981 caatccttgg tgaggacgat gcgtccgggg gccagctggt ccgccaggcc gaagagcgcg
     5041 ccataggggc aggcctggaa gcagaagccg cgactgccca gcaggtacac gatcacaaag
     5101 ccgcatacga agaaggtgag tacggccacg ccgggtggtg gcaggttgcg ccacatgtcc
     5161 tccgtagtga aggaggacca acgtgatccc cccgcttcca ccacatgcac ggcctgttgc
     5221 tccacgccat gccagagttg catgatctgc ggccagatga agagatagaa catcacggcc
     5281 atcggcaccc agatgagcgt ccgcgaccgg atgggttgcc gtttgatgcg caacttgtcc
     5341 atgatccatc cggccgcgtc ctgcaacgcg aggatgtggc agccccacga gcagaagaag
     5401 cgaccgaaga ccaacgtgcc cacc
//
LOCUS       DAOQOC010000042         5419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6100_length_5419_cov_3.435496, whole genome shotgun sequence.
ACCESSION   DAOQOC010000042 DAOQOC010000000
VERSION     DAOQOC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6100_length_5419_cov_3.435496"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1118)
                     /locus_tag="PL106_01220"
     CDS             complement(174..1118)
                     /locus_tag="PL106_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNA31714.1"
                     /translation="MERYQRLIGARVEAWCAALPEGGLYAPMAYLMGLPAKRVRPALT
                     LMACELFGGKAEDVLDEALGIELFHNFTLMHDDIMDAAPLRRGLPTVHEKWGVNTAIL
                     SGDAMLVKAYQLMGKRPEVLELFGRTALEVCEGQQLDMDFERREDVRVGECLAMLHRK
                     TAVLLACALRTGAMVAGAGTKDQERLVAFGEHIGLAFQLRDDLLDAFGDPANTGKQRG
                     GDLRAGKKTYLLIRGLERAAENRTGMLREELTKRPEQRDVERILTVLETLGVRDETEA
                     LVIKHHRMAFAQLMAVDAPEERKMPLIALADRLVDRVV"
     gene            complement(1118..1987)
                     /gene="rfbA"
                     /locus_tag="PL106_01225"
     CDS             complement(1118..1987)
                     /gene="rfbA"
                     /locus_tag="PL106_01225"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNA31715.1"
                     /translation="MKGIILAGGSGTRLHPLTLAVSKQLMPVYDKPMIYYPLSTLMAA
                     GIREVLIISTPHDLPSFRKLLGDGSALGCTFTYAEQKIPNGLAQAFVIGREHVGTDRV
                     ALILGDNIFHGSGLGTRLMEHRDVSGGLVFAYRVSDPERYGVVEFDQDGRALSIEEKP
                     SRPKSDFAVPGLYFYDNSVLDIARDLQPSARGEYEITDVNREYLRQGRLKVLVLDRGT
                     AWLDTGTFTSLMQAGQYVQVIEERQGRRIGCIEEVAFRRGFINAEQLVSLAQPLRKSG
                     YGEYLLKVAEGRA"
     gene            complement(1984..4416)
                     /locus_tag="PL106_01230"
     CDS             complement(1984..4416)
                     /locus_tag="PL106_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA31716.1"
                     /translation="MWEWLASRVLRNRTAILIGVLVVTVFMGYMATTVGMSYKHGGLL
                     PKTDSAYVEYERFKERFGEDGNTIVVGAEGHALYTPAHFKAWYELGNALGEIQGIDSI
                     FSEAHLFELVRDDSLRRFAARRVMDRPPVDQGEMDTLLARIRALPFYNGLLYNDSTGA
                     SLMMVFVNAAHFNTEGRSATVKAFGDRVDAFAKETGIPVHVSGLPWIRVKSTQLVKAE
                     MPVFMALSLFVCGLLLLLFFRSWRVTFICLGVVAVSVVWSFGSMGLLGYNITILQSVI
                     APLVIVTGVPNCVFLINAYHYEYVRHGNKVKALQRVISRIGAAAFMTNATTAVGFTTF
                     CVTDSDTLIEFGWVATIGIMVLWALSMLLIPILFSYFPPPKKRHLSHLDRRWLDRTVD
                     WIVNTSRTRRPLIYTTTGVLTVFAFIGIWRMKDESRIVDDLPADNPVLTDLRFFEANF
                     RGVMPLEVEIDTRKKGGVFTDAVLRRIDRLEDTLATYPQFSRSISIVDAVKFTKQAFY
                     GGDPGRYELLNNTEKPFILPYIEGARDKEGMARGFLDSTRQTTRLTVQMADVGTTRME
                     PLLAKLRTQVDSLFNPERYTVTLTGTCVTFLKGSSYLVSNLITSLFWAVVIIVALMAF
                     LFNSMRILIVALIPNIIPLLFTAGLMGFFGIPIKPSTMLVFGIALGIAVDNAIHFLAR
                     YRLELKLTNNDLRASVDLATREVSVGIIYTSVVLLAGFGLFGFSRFGGIRALGVLTTL
                     TLLVAMLTNLLVLPSLLLSLNRFILTRAFREPLLEILDEEEDIELMELKLDAPEPGPG
                     TVLNDDEQGPDA"
     gene            complement(4508..>5419)
                     /locus_tag="PL106_01235"
     CDS             complement(4508..>5419)
                     /locus_tag="PL106_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNA31717.1"
                     /translation="RIPEVGLGLKLITDHLPGVQVIATGSSAFELAGKVNEPLTGRKW
                     EYQLFPLSFGEMAAHHGLLEEERLLGHRLVHGYYPEVVAAAGNEREVLSTLANSYLYK
                     DVLAWQGIRKPDKLTKLLQALAFQIGNQVSYHELGQMAGLDNETVEKYILLLEQAFVI
                     FRLGSFSRNLRTELKRGRKICFVDNGIRNAVIAAYQPLDLRQDTGALWENFLMSERRK
                     YLMNAGTAGNAYFWRTTAQQEVDLVEEADGQLRAYEFKWNPKAKGRIPLSWQRAYPGS
                     MAEVIHRENYLPFLGMMEGKGDVVAEG"
BASE COUNT          981 a         1760 c         1652 g         1026 t
ORIGIN      
        1 gatgaccacg gcacgaaagg agcatgcgga catccgggcc atcgcgctct ccaagaacaa
       61 gaaacggttc tgcgatctct tcctgccctt cggcaaaggc ggtgtcacat tccagctact
      121 ggacgatccg gtactgttcc ggacgtactg cgagcactgc cagcactgaa tgcctacacc
      181 acccggtcca ccaggcggtc ggccagggcg atcaacggca ttttgcgctc ctccggggcg
      241 tcaaccgcca tgagctgtgc gaatgccatc cggtgatgct tgatgacgag ggcctcggtc
      301 tcatcgcgca cgcccaaggt ctccaacacg gtgagaatgc gctccacatc gcgttgttcg
      361 ggccgctttg tcagctcctc ccgcaacatc cccgtacggt tctccgctgc gcgttccagg
      421 ccgcgaatca agaggtatgt ttttttgccc gcacgtaggt cgccgccccg ctgtttgccc
      481 gtgttcgcag ggtcgccgaa ggcgtccagc agatcgtccc gcaactggaa ggccagcccg
      541 atgtgttcgc cgaaggcgac aagccgctcc tggtccttgg ttcccgctcc ggccaccatc
      601 gcacccgtgc gcagtgcgca ggccaacaat acggcggtct tccggtgcaa catggccagg
      661 cactcgccca cgcgcacgtc ttcgcgccgc tcgaaatcca tgtccagttg ctggccctcg
      721 cacacctcca gcgcggtacg gccgaagagc tccagcacct cgggtcgctt acccatcagc
      781 tggtacgctt tcaccagcat ggcatcgccg ctgaggatgg ccgtgttcac cccccatttc
      841 tcgtgcaccg tgggcaggcc gcgccgcagg ggggccgcgt ccatgatgtc gtcgtgcatg
      901 agggtgaagt tgtggaagag ttcgatgccc agcgcctcgt ccagcacgtc ctcggccttg
      961 ccaccgaaga gttcgcaggc catgagggtg agtgcgggcc ttacgcgctt ggccggcagg
     1021 cccatcagat aggccatcgg tgcgtacagg ccgccttcgg gcaacgccgc gcaccacgcc
     1081 tctacacgcg cgccgatgag ccgctggtac cgctccatca cgccctccct tcagccactt
     1141 tcagcaggta ctccccgtag ccgctcttgc gcaagggctg ggccaagctg accagttgtt
     1201 ccgcattgat gaatccgcgt cggaaggcca cttcctcgat gcaaccgatg cggcgccctt
     1261 gtcgttcctc gatcacctgc acgtactggc ccgcttgcat gagcgaagtg aaggtgccgg
     1321 tatcgagcca ggccgttccc cggtccagca cgagcacttt caacctccct tggcgcaggt
     1381 attcgcgatt gacgtcggtg atctcgtact cgccgcgcgc actgggttgc agatcgcggg
     1441 cgatgtccag cacgctgttg tcgtagaagt agagcccggg cacggcgaaa tcgctcttgg
     1501 gcctcgaagg tttctcctcg atgctcagcg cccgcccgtc ctgatcgaac tccaccacgc
     1561 cgtagcgttc ggggtcgctc acccgatagg cgaagacgag accgcccgac acatcgcggt
     1621 gctccatcag ccgtgtgccc aggccgctgc cgtggaagat gttgtcgccc aggatgagcg
     1681 ctacacggtc cgtgcctacg tgttcgcgcc cgatgacgaa tgcttgggcc aggccgttcg
     1741 gtattttttg ctcggcgtag gtgaaggtgc aacccaacgc cgaaccgtcg cccagcaact
     1801 tacggaagct gggcaggtca tgcggggtgc tgatgatgag gacttcccgg atacccgccg
     1861 ccatgagcgt gctgagcggg tagtagatca tcggtttgtc gtacacgggc atgagctgct
     1921 tgctcacggc caaggtcagc gggtgcaatc gcgtgccgga acctccggcg aggatgatgc
     1981 ctttcatgcg tccggtcctt gttcgtcatc gttcaggacg gtccccggcc ccggttcggg
     2041 tgcgtccaat ttcagttcca tcagttcaat gtcctcctcc tcatccagta tctcgagcag
     2101 cggctcccgg aaggcccgcg tgaggatgaa cctgttcagg ctgagcagca gggaaggcag
     2161 caccagcagg ttggtgagca tggccacgag cagggtcagc gtggtgagca cgcccagcgc
     2221 acggatccct ccgaaccggg agaatccgaa cagcccgaat ccggccagca ggaccacact
     2281 cgtgtaaatg ataccgacgc tcacttcccg ggtggccagg tccacactgg cccgcagatc
     2341 gttgttggtc agcttcagtt ccagccggta acgtgcgagg aagtggatgg cattgtccac
     2401 cgcgatgccc agcgcgatac cgaacaccag catggtgctg ggtttgatgg ggataccgaa
     2461 gaagcccatg agacccgccg tgaacagcag cgggatgatg ttggggatga gcgccacgat
     2521 gagaatgcgc atcgagttga acaggaaggc catgagggcc acgatgatga ccacggccca
     2581 gaacaagctg gtgatgaggt tgctcaccag gtagctgctg cccttgagga aggtgacgca
     2641 ggtgccggtg agggtcacgg tgtaccgttc ggggttgaac agcgaatcca cctgtgtgcg
     2701 cagcttcgcg agcaggggtt ccatgcgcgt ggtgcccacg tcggccatct gcacggtgag
     2761 gcgcgtggtc tgccgcgtgc tgtccaggaa gccgcgtgcc atgccctcct tgtcccgcgc
     2821 accctcgatg tacggcagga tgaacggttt ctcggtattg ttcagcaatt cgtaccgacc
     2881 ggggtcgccg ccgtagaagg cctgcttggt gaacttcacc gcgtcgacga tgctgatgct
     2941 gcggctgaac tgcggatacg tggccagcgt gtcctccagc cggtcgatgc gccgcagaac
     3001 ggcatcggtg aacacacctc cttttttacg ggtatcgatc tccacttcca gcggcatcac
     3061 accgcggaag ttggcctcga agaaccgcag gtcggtgagc accgggttgt cggccggcag
     3121 gtcgtccacg atccggctct cgtccttcat gcgccagata ccgatgaagg cgaacacggt
     3181 aaggaccccc gtggtggtat agatcaatgg tcgacgggtg cgcgaggtgt tcacgatcca
     3241 gtccaccgtg cggtccagcc agcggcggtc caggtggctc aagtgccgct tcttgggcgg
     3301 ggggaagtag ctgaagagga tcgggatcag cagcatgctc agcgcccaca ggaccatgat
     3361 gccgatggtg gccacccatc cgaactcgat gagcgtgtcg ctgtcggtga cgcaaaaggt
     3421 ggtgaagccc actgcggtgg tggcgttggt catgaaggcg gccgccccga tgcggctgat
     3481 cacccgttgc agcgccttca ccttgttgcc gtgccgcacg tactcgtaat gatacgcgtt
     3541 gatgaggaac acacagttgg gcacaccggt aacgatgacc agcggggcga tcaccgattg
     3601 caggatggtg atgttgtagc ccagcaggcc catggagccg aagctccaca ccacgctcac
     3661 cgccaccacc cccaggcaga tgaatgtgac gcgccaactg cggaagaaca ggagcagcaa
     3721 caggccgcac acgaacaggc tcaacgccat gaacacgggc atctccgctt tcaccagctg
     3781 ggtgctcttc acccggatcc acggcaggcc gctcacatgc acgggaatac ccgtctcctt
     3841 ggcgaaagca tcgacccgat cgccgaaggc cttcaccgtg gccgatcgtc cttcggtatt
     3901 gaaatgcgcc gcgttcacga agaccatcat caggctggcc ccggtgctgt cgttgtacaa
     3961 caagccgttg tagaatggca acgcccggat acgggccagc agggtatcca tctcgccctg
     4021 gtccaccggt ggccggtcca tcacgcgccg cgcagcgaac cgccgcagac tgtcatcgcg
     4081 caccagctcg aagaggtgcg cctcgctgaa gatgctgtcg atgccctgga tctcccccag
     4141 cgcgttgccc agctcgtacc acgccttgaa gtgggccggg gtgtaaagcg cgtgcccttc
     4201 ggccccaacc acgatcgtgt tgccgtcctc gccgaaccgt tccttgaagc gctcgtactc
     4261 cacgtacgca ctgtcggtct tcggcagcag acctccgtgc ttgtagctca tgcccacggt
     4321 ggtggccatg tagcccatga agaccgtgac caccagcacc ccgatcagga tcgccgtgcg
     4381 gtttcgcagt acgcggctcg ccaaccattc ccacatgctc gctcagacca gcccttgttc
     4441 gcgggccgcc gcgaaggtgc gcattagcgc ccggaatgcg gtgcggggaa agtccgggcc
     4501 tcctgcatca accttcggcc accacatccc cttttccctc catcatcccc aaaaaaggaa
     4561 gatagttctc ccgatggatg acctcggcca tgctgccggg ataggcgcgc tgccaggaga
     4621 gcggaatgcg ccccttggcc ttggggttcc acttgaactc gtaggcgcgc agttgcccat
     4681 cggcctcttc gaccaggtct acttcctgct gcgcggtggt gcgccagaaa tacgcgttgc
     4741 ccgcggtgcc cgcattcatc aggtacttgc gccgttcgct catcaggaaa ttctcccata
     4801 gcgcgcccgt atcctgccgc aggtccagcg gctggtaggc agcgatcacg gcattgcgga
     4861 tcccgttgtc cacgaagcag atcttgcggc ctcgcttcag ttcggtgcgc aggttgcgac
     4921 tgaaggagcc caggcggaag atgacgaagg cctgttcgag cagcaggatg tacttctcca
     4981 ccgtctcgtt gtccagtccg gccatctgcc ccagttcatg gtagcttacc tggttgccga
     5041 tctggaaggc cagcgcctgc agcagcttgg tgagtttgtc gggcttcctg atgccctgcc
     5101 atgccagtac gtccttgtac aggtagctgt tcgccagcgt ggacagcacc tcgcgctcgt
     5161 tccccgcagc ggccaccacc tccggatagt agccatgcac gagccggtga cccaacagac
     5221 gctcctcttc gagcaggccg tggtgcgcgg ccatttcacc gaaggagagg ggaaagagct
     5281 ggtactccca tttgcggccc gtgagcggct cgttcacctt gcccgccagt tcgaaggccg
     5341 atgaacccgt ggcgatgacc tgcacgccgg gcagatggtc ggtgatgagc ttgaggccca
     5401 gccccacctc ggggatccg
//
LOCUS       DAOQOC010000043         5416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6110_length_5416_cov_3.858422, whole genome shotgun sequence.
ACCESSION   DAOQOC010000043 DAOQOC010000000
VERSION     DAOQOC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6110_length_5416_cov_3.858422"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PL106_01240"
     CDS             <1..443
                     /locus_tag="PL106_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA31718.1"
                     /translation="RWGTLTTDDRAQVDRALRLTGADHLRQKALHTCSDGECQKVIIA
                     RAIAQDTPLILLDEPTAFLDLPNRAAIVRMLRQVAHEEGKCVLFSTHDLQLAIDLSDR
                     LLLISDGALWTGTHAEALNTGTLERTFAGSGIRFDSGGTHRFER"
     gene            complement(471..983)
                     /locus_tag="PL106_01245"
     CDS             complement(471..983)
                     /locus_tag="PL106_01245"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNA31719.1"
                     /translation="MMKSRIPLAMLVWATACSAPTPPPRTDPAPELPAVADPLALHRG
                     LLGDWVDRSSPRFTCFEHWSAQGDSMLNGFGYVLAKNDTVFVENLRIEAINGAVVYSA
                     RINSQNGGQWVPFTALPGGPDSLVYENPGHDFPQCITYVRDSTGGWEVTTTGNENGTE
                     RLERFHFVRL"
     gene            complement(980..1366)
                     /locus_tag="PL106_01250"
     CDS             complement(980..1366)
                     /locus_tag="PL106_01250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNA31720.1"
                     /translation="MAWGEQDLFGHVNNIVYFRYFESVRMHYLERIGVLRTHAESGLG
                     VILASTTCDFKKPVTWPQRLTIRTGMSRIGNTSFTMDYLITDEEGATVAIGTSVQVMY
                     DYRASSKIPITEEVRAAIARIQTVPT"
     gene            complement(1414..3345)
                     /locus_tag="PL106_01255"
     CDS             complement(1414..3345)
                     /locus_tag="PL106_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNA31721.1"
                     /translation="MSTKAALIERMEALLQSPDVEQAQEHVDTLKESYEALVAAARQE
                     AVAQHEAPVSIESAEGEAVAEPEVPEVPAQPIESAILQDEDDKKFKQLLDAFNTKVND
                     IRRQRQKQEADNLAAKQAIMEELRTMISSEENIGSAFQRFNELGEKWKTIGPVPQQNY
                     RDLQRDYSHLRDEFFYHIRIYKELRDHDLKKNTALKQALIADMEAVQKSETVKEAELR
                     VKEYQEKWHQIGPVVKEEWESIRDGFWNATRVVYDRINEHYRARRAEHDANLQAKEAL
                     IEKVRTLVAGMEAHNAKDWKALTDQILELQNAWKAIGFATKKDNERVWKEFRNACNTF
                     FDQKNTFFGELKGQYKAAYDKKQALLQEAIKLKDSTDWRQTADKLKVMQAQWKEAGSA
                     GPRDENKLWSRFREACDGFFQHRKAHFDALDAEQAVHVVDKEQLLSELENFQHLPDRS
                     ATLDALKAFSARWMNSGRVGPKVFDKMNARYRAAMDKQYGLLRVNAEERRRMAFTSRV
                     DDLKAAPDGEHRLEKEARFIKRKIEEIEGEMKQMDRNMGMFNFKSASGEAMKKDMEKK
                     IERARQDIERLRGQHRELVREIRGEAAPKPATNATAASVGSTQEEPVVPSPPDPAAEE
                     TVAPITPPAGEQATEATAS"
     gene            complement(3424..>5416)
                     /locus_tag="PL106_01260"
     CDS             complement(3424..>5416)
                     /locus_tag="PL106_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNA31722.1"
                     /translation="PWTFFGIMVLGTGILMGGAWAYEALSFGGFWAWDPVENGSLVPW
                     IVLVGSGHLMLINKRKPTSLFTTLLLVLGTWLLVLYSTFLTRSGVLGDTSVHSFTGEG
                     MMPGLLTLILTFVVLGTVMLNRDRQVRLWYTGLSTVLLLLGWWTDRLSSAILIFAGLT
                     LATTVVAYGSRTAFKDTGEEEVTSREFWLFIGSLVLLLSAVQITWTTSIPVYNLLLEP
                     LGDAVKRAPPVESIPHYNKWQIPFAFIVSLLVAVGQYLRYGSTDVRRCMRELALSLLG
                     SVLITGLCVWLLRYRANEATVVALIFATSFAALANTAYIFRVLKGSLRNAGPSIAHTG
                     FALVLLGATISTSKQEAISRNVKGPRLSTMSNEFNDSEEMLLYKGDTVPIGDRFVTYT
                     GSRKDGVNLHFDMDYLRAVPRHFKAGDTVRVRNSIFVATDDHVAGNSFLLDQPTHWRP
                     LDSAAHRVYWHASAWSSTLPAGKEFSLEPFVQLNPRFGNVAEPSTRHWPQRDLYTHIR
                     YAKLDPEDTTDFMPPRLFEKSVGDTIITPTCLIVVDSVRAVRDSVIARRLGPEFVAYA
                     PILKVRDLYDPTRWFEARPVIIYRHDQVVGSKGFGIPALNMKLDVSSVKDGVIGINVY
                     EREFVIMQAIVFPGINVLWIGCVLMAIGTGMAVWRRVRR"
BASE COUNT          903 a         1705 c         1700 g         1108 t
ORIGIN      
        1 gtcgttgggg cacgctcacg accgacgatc gcgcccaagt ggaccgcgca ctccggttga
       61 ccggtgcgga tcacttgcgc cagaaagccc tgcacacgtg cagcgatggc gaatgccaga
      121 aggtgatcat cgcccgggcc atcgcccagg acacgcccct gatcctgctt gacgagccca
      181 cagccttcct cgacctgccc aaccgggccg ccatcgttcg catgctccgc caggttgcgc
      241 acgaggaggg gaaatgcgtc ctcttcagca cgcacgacct ccaactggcc atcgacctca
      301 gcgatcgcct gctcctgatc agtgatggtg cgctatggac cggcacgcac gccgaggcgc
      361 tcaacaccgg taccttggaa cggacgttcg cgggaagcgg tatccgcttt gactcgggcg
      421 gcacgcaccg gttcgagcgg tgaggggaga agacggaggc gacgccatcc ttacaaccgc
      481 acgaaatgga accgctccag cctttccgtg ccgttctcgt tcccggtggt ggtcacctcc
      541 cagccgcccg tgctgtccct gacgtaggtg atgcactgtg ggaagtcatg cccggggttc
      601 tcgtacacca gtgagtcggg gccgccgggc agggcggtga acggtaccca ctgaccaccg
      661 ttctggctgt tgatccgggc cgaatacacc acggcaccgt tgatcgcctc gatacgcaga
      721 ttctcgacaa aaacggtatc atttttcgcc agcacgtaac cgaagccgtt gagcatgctg
      781 tcgccctgtg cgctccagtg ttcgaagcag gtgaagcggg gcgagctccg gtccacccaa
      841 tcgccaagga gcccccggtg gagcgccaat ggatcggcaa cggccggaag ctccggagcg
      901 ggatccgtac ggggtggggg agtcggcgct gagcaggccg tggcccacac cagcatggcc
      961 agtggaatgc ggcttttcat catgttggaa cggtctggat gcgtgcgatg gccgctcgta
     1021 cctcctcggt tatagggatc ttgctacttg ccctgtagtc gtacatcacc tgcacactgg
     1081 tcccgatggc caccgtcgct ccttcctcat cggtgatcag gtagtccatg gtgaaactcg
     1141 tgttcccgat gcggctcata cctgtccgga tggtgagtcg ttggggccag gtcacgggtt
     1201 tcttgaaatc gcacgtggtg ctggccagga tcacgccgag gccgctctcc gcgtgtgtgc
     1261 gcagcacgcc gatccgctcc aggtagtgca tgcgcacgct ttcaaaatag cggaagtaga
     1321 cgatgttgtt cacatgcccg aacaggtcct gttcgcccca ggccacgggt acttccaagc
     1381 tgacaacgag gtgcgctgaa gacctcacac ggatcaggaa gctgtcgctt cggtggcttg
     1441 ttcgccggcc ggtggagtga tgggtgcaac ggtctcctcc gcagcgggat caggcggcga
     1501 cggtacaacg ggttcctcct gagtggaccc cacggatgcg gcggttgcgt tcgtggccgg
     1561 tttgggcgcg gcctcaccgc gtatctcccg cacgagttcg cgatgctgac cgcgcagccg
     1621 ttcgatgtcc tgccgtgccc gctcgatctt cttctccatg tccttcttca tggcctcgcc
     1681 actggcgctc ttgaagttga acatgcccat gttgcggtcc atctgtttca tctcgccctc
     1741 gatctcctcg atctttcgct tgatgaaacg cgcctccttc tccaggcgat gctcgccatc
     1801 gggcgcggcc ttcaaatcgt caacacggct cgtgaatgcc atgcgtcggc gttcctcggc
     1861 gttgacacgc aacagcccgt actgcttgtc catggccgcc cggtaacgcg cgttcatctt
     1921 gtcgaacacc ttggggccca cccggccgct gttcatccag cgggcactga aggccttcag
     1981 cgcgtcgagc gtggccgaac ggtccgggag atgttggaaa ttctccagtt ctgaaagtag
     2041 ttgctccttg tcgaccacgt gtacggcctg ctcggcgtcc agcgcatcga agtgggcctt
     2101 gcggtgctgg aagaaaccgt cgcacgcctc gcggaatctg ctccagagct tgttctcgtc
     2161 tcgcggaccg gcgctcccgg cttccttcca ttgcgcctgc atcaccttca gcttgtcggc
     2221 cgtctggcgc cagtcggtgc tgtccttcag cttgatggcc tcctgcagga gcgcctgctt
     2281 cttgtcgtac gccgccttgt actgcccctt cagctcaccg aagaacgtgt tcttctggtc
     2341 gaagaaggtg ttgcaggcgt tgcggaattc cttccacacg cgctcattgt ccttcttggt
     2401 ggcgaagccg atggccttcc acgcgttctg cagctccagt atctggtcgg tgagggcctt
     2461 ccaatccttg gcgttgtgcg cctccatgcc ggccaccagg gtccgcacct tctcgatcag
     2521 ggcttccttg gcttgcaggt tggcgtcatg ttccgcacgg cgtgcgcggt agtgttcgtt
     2581 gatccgatcg tacaccacgc gcgtggcgtt ccagaacccg tcgcggatgc tttcccactc
     2641 ctccttcacc acgggaccga tctggtgcca tttctcctgg tattctttca cccggagctc
     2701 ggcttccttc acggtttcgg acttctgcac ggcctccatg tcggcgatga gcgcttgctt
     2761 gagcgccgtg ttcttcttga ggtcgtgatc gcgcagttcc ttgtagatgc ggatgtggta
     2821 gaagaactcg tcccgcaggt ggctgtagtc gcgctgcagg tcgcggtagt tctgctgcgg
     2881 cacggggccg atggtcttcc acttttcgcc cagctcgttg aaccgctgga aggccgagcc
     2941 gatgttctcc tcggaactta tcatggtgcg caattcctcc atgatggcct gcttcgcggc
     3001 caggttgtcg gcctcctgct tctggcgctg acggcggatg tcgttcacct tcgtgttgaa
     3061 ggcgtcgagg agctgcttga attttttgtc atcctcgtct tgcagtatgg cactctcgat
     3121 gggttgcgcg gggacctcgg gcacttcggg ctccgcgacc gcctcgcctt ctgcactttc
     3181 gatggacacg ggtgcttcgt gctgcgcaac ggcttcctgg cgggccgccg ccacgagtgc
     3241 ttcgtaggac tccttcaggg tgtccacatg ttcctgggcc tgttccacat cgggactttg
     3301 gagcagggcc tccatgcgct cgatgagcgc tgctttcgtt gacatcggat cggctgcggc
     3361 gaaagggaag ggcggccgca cggccaaaga taggaggtgg cccgctacca atgggcgcgc
     3421 gtcctatcgc cgtacgcgcc gccacaccgc catgcccgta ccaatggcca tgagcacgca
     3481 gccgatccat agcacgttga tgcccgggaa gacgatggct tgcatgatca cgaattcgcg
     3541 ctcgtacacg ttgatgccga tgacaccgtc cttcacgctg gatacatcca gcttcatgtt
     3601 caaggcgggg atcccgaatc ccttgctgcc taccacctgg tcgtgccggt agatgatcac
     3661 gggtcgtgct tcgaaccagc gcgtggggtc gtacaggtcg cgtaccttca ggatgggagc
     3721 gtatgccacg aattccggtc ccagccgccg ggcgatcacg ctgtcgcgta ccgcacgcac
     3781 gctgtccacc acgatcaggc aggtgggtgt gatgatggtg tcgcccacgc ttttctcgaa
     3841 caggcgcggc ggcatgaaat cggtggtgtc ctcggggtcg agcttggcgt agcggatgtg
     3901 cgtgtacagg tcgcgctgcg gccagtgacg ggtactgggc tcggccacgt tgccgaagcg
     3961 tgggttcagt tgcacgaagg gctccagcga gaactccttc ccggccggta gcgtgctgct
     4021 ccatgcggac gcgtgccagt acacccggtg ggcggcgctg tccagtggac gccagtgggt
     4081 gggctggtcc aacaggaagc tgttgcccgc cacgtggtcg tcggtggcca cgaagatgga
     4141 gttccgtacc cgcaccgtgt cgcccgcctt gaagtggcgc ggtaccgcac ggaggtagtc
     4201 catgtcgaaa tgcaggttca ccccgtcctt gcgcgagccc gtgtaggtga cgaagcgatc
     4261 gccgatgggc acggtgtctc ccttgtacaa cagcatctcc tcgctgtcgt tgaactcgtt
     4321 gctcatggtg ctcagccgcg ggcccttcac attacggctg atcgcttcct gtttactggt
     4381 actgatggtg gcgcccagca gtaccagtgc gaagcccgtg tgcgcgatgc tcgggccggc
     4441 gttgcgcaag ctgcccttca gtacacggaa gatgtacgcc gtattggcca gcgcggcgaa
     4501 ggatgtggcg aagatgagcg cgaccaccgt ggcctcgttc gcgcggtagc ggagcagcca
     4561 cacgcacaag ccggtgatca gtaccgagcc cagcaacgag agggccagtt cgcgcatgca
     4621 gcgccgtacg tcggtgctcc cgtaacgcag gtattggcca acggcaacca gcagcgatac
     4681 gatgaacgcg aaagggatct gccatttgtt gtagtggggg atggactcca ccggcggcgc
     4741 gcgcttcacg gcatcgccca ggggctccag cagcaggttg tacaccggga tggaggtggt
     4801 ccacgtgatc tgcacggcgc tgagcagcag caccaacgag ccgatgaaga gccagaactc
     4861 gcggctggtg acctcctcct cgccggtgtc cttgaaggcg gtgcgcgatc cgtaggccac
     4921 caccgtggtc gccaacgtga gtccggcgaa aatgaggatg gcgctcgaga ggcggtccgt
     4981 ccaccatccg agcagcagca gcacggtgct gagcccggtg taccacaggc gcacctgccg
     5041 gtcgcggttg agcatgacgg tacccagtac cacgaacgtg aggatgagcg tcagcaagcc
     5101 cggcatcatg ccctcgccgg tgaagctgtg gacgctggta tcgcccagca cgccgctgcg
     5161 ggtgaggaag gtgctgtaca gcacgagcag ccaggtgccc agcaccagca ggagcgtggt
     5221 gaagagggag gtgggcttgc gcttgttgat gagcatgaga tgccccgaac ccacaagaac
     5281 gatccatgga acaagggatc cgttctccac ggggtcccag gcccagaagc cgccgaaact
     5341 gagggcctca tacgcccaag caccgcccat gaggatgccc gtgcccagca ccatgatgcc
     5401 gaagaaggtc cacggc
//
LOCUS       DAOQOC010000044         5396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6145_length_5396_cov_3.377645, whole genome shotgun sequence.
ACCESSION   DAOQOC010000044 DAOQOC010000000
VERSION     DAOQOC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6145_length_5396_cov_3.377645"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..1437)
                     /locus_tag="PL106_01265"
     CDS             complement(340..1437)
                     /locus_tag="PL106_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNA31723.1"
                     /translation="MLNALFQPFTLRTLTLKNRFVMAPMTRSFSPGGIPGPDVAAYYR
                     RRAEGDVGLILSEGTVIERASSSNDPDVPHFHGEAALHGWKHVIDEVHAAGGRMGPQL
                     WHMGVMPLHKSGWSPPVPFEGPSDRINTEKRNGRAMSDADIADTIAAFGRAAHHAQAL
                     GFDLVELHGAHRYLIDQFFWNATNTRTDRYGGTTLAERTRFACEVIREVRRQVGQRMV
                     VQIRISQWKPGAYDNKLATTPREMEAWLTPLIEAGADLLHCSQRRFWEPEFDGSDLNF
                     AGWAKKLTGIPVITVGSVGLSGEFTAAFRGDSSTATPLDELVRRFDRGDFDLVAVGRA
                     LLMDPHWTRKIRDGRTNELLGFSKEALQNLR"
     gene            1652..4264
                     /locus_tag="PL106_01270"
     CDS             1652..4264
                     /locus_tag="PL106_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNA31724.1"
                     /translation="MTLSTPFRTLILATALTTAPMLLAQEAAYIPGDILLMLEPGASA
                     RSVVNDLAFINGQPTGLRVVKEVSAPMRAWLLHYDNPALRQAEVMRQVGRHPGVTFAQ
                     NNHVVKDRAVPNDPQYGQQWQHQNIDSEAAWDITTGGLTATGDTIVVCIIENADLPHP
                     DLVANAWYNHQEIASNSIDDDGNGYVDDFQGWNPGGNNDDVYGGGHGTQVAGMIGAVG
                     DNGSQVAGANWDVKMMVVTRDGIGEAAVVESYTYPLVMRRRYNETNGAEGAFVVATNA
                     SWGIDNADPAGYPIWCAIYDTLGAAGVLNCGATANNNVNIDVVGDMPTACASDFMVSV
                     TATNTNDMRTFSGYGATTVDLGAPGENIFTTSMGGGTGSTSGTSFASPLTAGVIALLY
                     SAPCATMMGLVHADPEAGALYVRQALFTGVEQVGNLPGNTVTGGRVNSNNSLLWIMSG
                     CGTCPAPYNLSGSSTALGEEDLVWSSTVGTTFNVRYRPVGSTDWVVVNDVPSASLTLQ
                     DLDLCAAYEYQVEAQCEGETSGWSASWTFTSEGCCTVPTSVTAEAVDTTGINVQWSVV
                     LAANTYDIRYALAGTTDWTTVAGLSTSPYAITGLEGCNEYEVQMRTSCDGITTTDWSA
                     STLVMVPGCGVCVDLAFCPSVSASTNDEWIDRVRIGAIDNTSGNNEGYALFATESTTL
                     DIGTPTPVILTPGYDSDEYAEAWTIWLDLDLNGQFEAPGERVFQSDAVAGEVTGNLLV
                     PETAPVGPTRMRVIMEYFSAAATGCENDYNYGETEDYCVTIAPASGLTAVEGHGTSAY
                     PNPADDAMTIALPGAGRAVIEVLDNTGRMAMRTSADGPRAWVTTSNLAEGLYLYRVMV
                     GGRETARGKFQVAH"
     gene            4337..5095
                     /locus_tag="PL106_01275"
     CDS             4337..5095
                     /locus_tag="PL106_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA31725.1"
                     /translation="MALSVVIITRNEERNIARCINSVRTLSDDIVVVDSGSTDRTVAI
                     ATELGARVSMHTWAGYSAQKNHANALARHAYILSLDADEALSPELTASIRTAEQAGWH
                     GAYGFNRLTNYCGRWVRHGGWYPDVKIRIFPKASARWTGDHVHETLELDPGTRVNHLA
                     GDLLHWSYHSLSDHAERIERYSTLHARKMLAEGKRAGWVKRRLSPLFKFVQGYVFQMG
                     LLDGSAGFHIARYSARAVALKYAKLHQLLAEHKA"
     gene            5092..>5396
                     /locus_tag="PL106_01280"
     CDS             5092..>5396
                     /locus_tag="PL106_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31726.1"
                     /translation="MSMPGTILISRTDSIGDVMLTLPLAGILKEQYPGTEVVFLGKGY
                     TAPVLACCSHVDRVVTREDLVAGGVGALKDIGADAIIHVLPDRALAWLAVRAGIPVR"
BASE COUNT         1029 a         1619 c         1778 g          970 t
ORIGIN      
        1 accgttgaac aggtagcttg gaacgccgtg gtcccaagcg cgttgcagca cgcctgcctg
       61 gggttggtcg cagccgagga gggcgacttc agcctgcgta tgcccatgga aatgttccat
      121 gaggcgctgg gcgttggtgc ccgaaccgga ggcgaggatg gcgatgcgga tcatgggcct
      181 gaacacaggt gcggccacat gctcacgtga gatctggccg tccgtgggcg cgaaggtccg
      241 gaaaacgggt atgcaccgtg ttctcggagg aacatccggg cgtccggccc ccgcttcttg
      301 cctcagcctg gccattttac acgaagaacg atcctgggct cacctcaggt tctgaagggc
      361 ctccttgctg aagccgagca gttcgttggt gcggccatcg cggatcttcc gtgtccagtg
      421 cggatccatc agtaacgctc ggcccacggc caccagatcg aagtcgccgc gatcaaagcg
      481 acgaaccagt tcatccaatg gggtcgctgt cgagctgtcg ccacggaaag ccgccgtgaa
      541 ctcgccactg agacccaccg accccacggt gatcacagga atgccggtga gctttttggc
      601 ccatccggcg aagttgaggt cgctgccgtc gaactcgggt tcccagaagc ggcgttgcga
      661 gcagtgcagc aggtcggcgc cggcttcgat cagcggggtg agccaggctt ccatttcccg
      721 gggtgtagtg gccaacttgt tgtcgtaggc cccgggtttc cattgcgaga tgcggatctg
      781 cacaaccatc cgctggccca cctgccgacg cacttcgcgg atcacctcgc aggcaaagcg
      841 cgtacgctcg gcgagtgtgg ttccgccata gcgatcggtg cgcgtgttgg tcgcgttcca
      901 gaagaactga tcgatcaggt aacggtgcgc gccgtgcagt tccaccaggt cgaagcccag
      961 ggcctgcgcg tggtgtgccg cccgtccgaa cgcagcgatg gtatcggcga tgtccgcatc
     1021 ggacatggcc cgtccgttgc gtttttcggt attgatgcgg tcactgggtc cctcgaaggg
     1081 cacaggcggc gaccacccgc tcttgtgcag gggcatcacg cccatgtgcc agagctgcgg
     1141 acccatgcgc ccgccagcgg catgcacctc gtcgatcacg tgtttccagc cgtgcagcgc
     1201 cgcttcgcca tggaaatgcg gtacatcggg atcattgctc gacgaagccc gctcgatcac
     1261 cgtgccttcg gaaagaatga gccccacatc gccttcggca cggcggcggt agtaggcggc
     1321 cacatcgggg cccgggatac cgccgggcga gaaggaccgc gtcatcgggg ccatgacgaa
     1381 ccggttcttc aaagtcagcg tccgcagcgt gaagggctga aagagtgcgt tgagcatggc
     1441 gcgaatctcc gcacagtgga ccaacccatg ccgaaatacc cctgttgggt ccggacgtga
     1501 agcgcatggt agaccaggtg cggcgcctgg taaaggtgga agccttccag atccttccgc
     1561 aaatgggccg catccgggcc atcgggaaga cgtggtccat ctaccttagc cgaaactttc
     1621 ggaccgaaag accctaccaa cccgaccgcg catgacccta tcgaccccgt tccggacgct
     1681 gatcctggcc actgcactta ccactgcgcc catgttgctg gcccaggaag ccgcgtacat
     1741 ccccggcgat atcctgttga tgttggagcc cggagcttca gcacgatcgg tggtgaatga
     1801 cctcgcgttc atcaatggcc agccgacggg gttgcgtgtg gtgaaagagg tgagtgctcc
     1861 catgcgtgcc tggttgttgc attacgacaa tccggccctt cgccaggctg aggtgatgcg
     1921 tcaggtgggt cggcacccgg gggtcacctt cgcgcagaac aaccacgtgg tgaaggatcg
     1981 tgcggtaccc aacgacccgc agtacggaca acagtggcag catcagaaca tcgacagtga
     2041 agcggcttgg gacatcacta cgggggggct cacggccacg ggcgatacca tcgtggtgtg
     2101 catcattgag aacgcggatc tgccgcatcc ggatcttgtg gccaatgcgt ggtacaacca
     2161 tcaggagatc gcgagcaaca gcatcgacga cgacggcaac ggctatgtgg acgacttcca
     2221 aggctggaat ccaggtggga acaacgacga cgtgtacggg ggcggccatg gcacccaagt
     2281 ggcgggcatg atcggtgcag tgggcgacaa cggcagccaa gtggccgggg ccaattggga
     2341 cgtgaagatg atggtggtga cgcgcgatgg catcggcgag gccgccgtgg tggagagtta
     2401 tacctatcca ctggtcatgc gtcggcggta caacgagacc aatggagcgg aaggggcgtt
     2461 cgtggtggcc acgaatgcca gctggggcat cgacaatgcc gacccggccg gctatcccat
     2521 ctggtgcgcc atttacgaca cgctcggtgc ggcaggcgtc ctgaactgcg gggccacggc
     2581 caacaacaac gtgaacatcg acgtggtggg cgacatgccc acggcttgtg cgagtgactt
     2641 catggtgagc gtaacggcaa cgaacaccaa cgatatgcgc accttcagcg gatatggtgc
     2701 caccaccgtt gatctgggcg caccgggcga gaacatcttc accaccagca tgggcggggg
     2761 cacgggcagc accagcggca cctccttcgc cagtccgctc accgccgggg tgatcgccct
     2821 gttgtacagc gcgccctgtg ccaccatgat gggcctggtg catgccgatc cggaggccgg
     2881 agccctgtac gtacgtcaag cgctctttac cggtgtggag caggtgggca acctgccggg
     2941 caacaccgtt acaggtggtc gcgtgaacag caacaacagc ctgctatgga tcatgtccgg
     3001 ctgtggtacg tgccccgctc cgtacaacct gtccggttcg agcaccgcgt tgggcgagga
     3061 ggacctggtg tggagcagta cggtcggcac cacgttcaac gtgcggtacc ggcctgtggg
     3121 cagtactgac tgggtcgtgg tcaacgacgt gccgagcgca tcactcaccc tgcaggacct
     3181 ggacctttgc gcggcctatg aataccaggt ggaggcccaa tgcgaaggtg agaccagtgg
     3241 ctggagcgcc tcctggacct tcacctccga aggttgctgc accgtgccga cgagcgtgac
     3301 cgccgaggcc gtggacacca ccggtatcaa cgtgcagtgg agcgtggtgc tggcggccaa
     3361 cacctacgac atacgatacg cattggccgg taccacggac tggaccacgg tcgcaggtct
     3421 gagcacatcg ccgtacgcga tcaccgggct cgaaggctgc aacgaatacg aggtgcagat
     3481 gcgcaccagt tgcgatggca tcaccaccac ggattggagc gcctccacct tggtgatggt
     3541 acctggctgt ggtgtgtgcg tggacctggc cttctgcccc agtgtttcgg ccagcaccaa
     3601 tgacgaatgg atcgatcggg tgcggatcgg tgccatcgac aacacgagcg ggaacaacga
     3661 gggctacgcg ctcttcgcca cggagagcac gacactggac atcggcactc ctactcctgt
     3721 gatccttacg cccgggtatg acagtgatga gtacgccgag gcgtggacca tctggctgga
     3781 cctggacctg aatgggcagt tcgaggctcc cggcgaacgg gtattccaat ccgatgccgt
     3841 tgcgggagag gtcacgggca acctgctcgt accggaaacg gcccctgtcg gccccacacg
     3901 catgcgggtc atcatggagt acttctccgc ggctgccaca ggctgcgaga acgattataa
     3961 ttacggggag accgaggact attgtgtgac cattgctccc gcatcgggac tgaccgccgt
     4021 ggagggtcat ggcacgagtg cctacccgaa tccggcggac gatgcgatga ccatcgcact
     4081 tccgggagcg ggccgtgccg tgatcgaagt actggacaac accgggcgta tggccatgcg
     4141 cacctcggcc gatggcccac gcgcatgggt caccacatcg aacctggccg aggggctcta
     4201 cctgtatcgc gtgatggtcg gcggtcgtga gaccgcacgc ggcaagttcc aggtggcgca
     4261 ctgaccacac cgggtgaagg cccctcttcc atcgcacggt ggggaggggc cttttcgcgg
     4321 gataccttgg ccgcccatgg cgctcagcgt cgtcatcatc acccgtaacg aggagcggaa
     4381 catcgcgcgc tgcatcaact cagtgcgtac gttgagcgac gacatcgtgg tggtggactc
     4441 gggcagcacc gaccgcacgg tggccatcgc aacggagctt ggcgcaaggg tcagcatgca
     4501 tacgtgggcg ggctacagcg cgcagaagaa ccatgccaac gcgctggcac gtcacgcgta
     4561 catactttcg ctcgatgcgg atgaggcgct ctccccggag ctgacggcct ctattcgcac
     4621 cgcggaacaa gcgggatggc acggggcgta cgggttcaac cgtctgacga attactgcgg
     4681 tcgttgggtg cgtcatggcg gttggtatcc ggatgtcaag atccgcatct tcccgaaagc
     4741 gtcggcacgt tggacgggcg atcatgtgca tgagaccctc gaacttgatc ccggaacacg
     4801 ggtaaaccac cttgcgggag acctgttgca ctggtcctat cacagcttgt cggatcacgc
     4861 cgaacgcatc gagcggtaca gcacactgca tgcgcgcaag atgttggccg agggcaagcg
     4921 ggctggatgg gtcaaacgtc ggttgtcccc gctgttcaaa ttcgtccagg gctatgtctt
     4981 ccagatgggc cttcttgatg gttcggcggg cttccacatc gcccgttatt cggcgcgggc
     5041 ggtggcactg aagtatgcca agctccacca attgctcgcg gagcacaagg catgagcatg
     5101 ccgggtacca tcctcatcag tcgtacggac agcatcggcg acgtgatgct cacgctgccg
     5161 ttggcgggga tcctgaagga gcaatacccg ggaacggagg tcgtgtttct ggggaaaggg
     5221 tatacggcac cggtgctggc gtgctgttcg catgtggatc gggtggtgac acgtgaggac
     5281 ctggtggcag gtggggtggg ggcgctgaag gacatcggtg ccgacgcgat catccacgtg
     5341 ttgcccgatc gggcgctcgc ctggctggcg gtgcgggccg gcatcccggt gcgcat
//
LOCUS       DAOQOC010000045         5377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6186_length_5377_cov_4.018038, whole genome shotgun sequence.
ACCESSION   DAOQOC010000045 DAOQOC010000000
VERSION     DAOQOC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6186_length_5377_cov_4.018038"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PL106_01285"
     CDS             <1..438
                     /locus_tag="PL106_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNA31727.1"
                     /translation="NGTAHCDNHTYIGHDVPDCTSDELYKGIVTEKATGVFNGKVYVA
                     PDAQRTRAYQNNANILLGDNAKVYTKPELEIYADDVKCSHGCTIGRLDEKGLFYLRSR
                     GVSEVEARRMLAHAFMTDVIERIANQDWRAHLANIIDAKLETL"
     gene            435..1706
                     /locus_tag="PL106_01290"
     CDS             435..1706
                     /locus_tag="PL106_01290"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNA31728.1"
                     /translation="MSTKASTVEKKGYDVEGIRAQFPILRELVHGKPLVYFDNAATSQ
                     KPRRVIKAESAYYATINANVHRGVHTLSTKATEAQEAARETIRKHIHAEHVHEIIFTS
                     GTTASINLAAASLGQLLLKKGDEVVISGMEHHSNIVPWQLACERHGAMLKVIPITDNG
                     EWDLGAIPAQGPNDNALLSERTRILAISHVSNTMGTINPVKKLIAQAKKQGITTVVDG
                     AQAMPHLNIDVQDLGCDLYAFSGHKAYGPTGTGVLYGREELMGCMPPWQGGGEMIDTV
                     TLEKSTWAKLPFKFEAGTPHIAGIIGLGEAVRWMEDYDKSAMAVHEHQLIEHATKELL
                     AIEGLRIIGTAKEKAGVISFVIEGVHHYDLGTLLDQQGIAVRTGHHCTQPLMDRFGVS
                     GTTRASFACFNTIAEVDALVAGVKKAVKMLR"
     gene            1762..2226
                     /locus_tag="PL106_01295"
     CDS             1762..2226
                     /locus_tag="PL106_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNA31729.1"
                     /translation="MENMTNDPVKRDWNMNGLYDLGETISVVAEQDGRKVNNFKDLWV
                     WKTGMELLKPVYQVTRKLPKEELYGLTSQLRRAAVSVPSNIAEGWGRNKKGYLLLGLS
                     YSRGSLHEIETQLLACVELEFLKHEDIQPLLDKIAGISTGLLRFMNNLESKR"
     gene            2287..2706
                     /locus_tag="PL106_01300"
     CDS             2287..2706
                     /locus_tag="PL106_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNA31730.1"
                     /translation="MSLAQTQQDLVEEFALFTDWQDRYEHLIELGKDLPLIAPENKVD
                     ANLVRGCQSRVWLHAELKNGVVHLTADSDAMITKGIVALLVRVYNDRTPQEILGASTD
                     FIDRIGLREHLSPNRANGLSAMIDQMKRYALAYSSNS"
     gene            2703..3023
                     /locus_tag="PL106_01305"
     CDS             2703..3023
                     /locus_tag="PL106_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNA31731.1"
                     /translation="MTPEDKHQLEESIINALKSVYDPEIPVDIYELGLIYDVVVHDDA
                     SVYIKMTLTSPACPVAGSLPGEVEQKAAGVQGVKSAKVELTFEPPWDRTMISEAAQLE
                     LGFM"
     gene            3094..4131
                     /locus_tag="PL106_01310"
     CDS             3094..4131
                     /locus_tag="PL106_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31732.1"
                     /translation="MLTRYASSTFALLSVLSSSAQLSILTQFDPSNTAGTCGIAYNAP
                     DGEIMVIGCSASVIDRYTEAGTFLSSANVVGEGANDVDIDMAPADLVMNGTFVAQGQL
                     LLVNGEVGVAEVYAIDEVTNEVIDTLVSGFGVSHVVGGSYHSQRGTFFLVQDIQPGGF
                     DRNRIAEIDATTGDTLRTFSLDPYFDVNYGDLDISQVSGNLFLVSSEESGIAEFTPDG
                     MFIAEHPLPSGVSGLSGIALDNDLEGAWVCNTSGVVYKLGNFPMGTFGNIGAPDLHVH
                     PNPTSDVLLVTCDRASTNDVIAIVDAAGRTLLRERITNALMRLDVSPLPAGPYIMRLE
                     ASGKSRRFMKD"
     gene            complement(4137..5198)
                     /gene="pdxA"
                     /locus_tag="PL106_01315"
     CDS             complement(4137..5198)
                     /gene="pdxA"
                     /locus_tag="PL106_01315"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNA31733.1"
                     /translation="MSEKTMRVGISCGDLNGVGLEVVLKAFEDNRMHQDITPVLYCGV
                     KAVSHHRKALKLDDMQIHGVKSVNDIIPRKFNVVDLWDEDVPIELGKPSGQQSKYAIQ
                     SLEAAAQDLGQGKIDMLVTAPIDKHSMQLAGFQFPGHTEFLSHLAGSDSPVLMILVGD
                     GLRVALVTGHLPINQVAEAVTTERIIGKARLFNRSLQSDFGINQPRIAVLGLNPHAGD
                     GGAIGSEDKERIAPAVRKLQEEGINAMGPYPADGFFGNGSYKHFDGVLAMYHDQGLAP
                     FKALSFGHGVNYTAGLPIVRTSPDHGTGLGIAGRNEADPGSLRHAVWLGLDIIRNRER
                     WKRMSANPLQPQKKEKGGE"
BASE COUNT         1134 a         1575 c         1653 g         1015 t
ORIGIN      
        1 aacggtaccg cgcattgcga caatcacacg tacatcggac atgatgtacc ggattgcacc
       61 agcgacgagc tctacaaggg catcgtgacg gagaaggcca ccggcgtgtt caacggcaag
      121 gtgtacgtgg cgcccgatgc ccaacgcacc cgggcgtacc agaacaacgc caacatcctg
      181 ttgggcgaca acgccaaggt gtacaccaag cccgagttgg agatctacgc ggacgatgtg
      241 aagtgcagcc acggttgcac catcggccgc ctggatgaga agggcctgtt ctacctgcgt
      301 tcgcgcgggg tgagcgaggt ggaagcgcgc cgcatgctcg cgcacgcgtt catgaccgat
      361 gtgatcgagc gcatcgcgaa ccaggactgg cgagcgcacc ttgcgaacat catcgatgcg
      421 aaattggaaa cgctgtgagc accaaggcca gcacggttga gaagaagggc tacgacgttg
      481 aggggatccg tgcgcagttc ccgatcctga gggagttggt ccacggcaag ccgctggtgt
      541 acttcgacaa tgcggccacc agccagaagc cgcgccgcgt gatcaaggcg gagagcgcgt
      601 actacgccac gatcaacgcg aacgtgcatc gcggtgtgca taccttgagc accaaggcca
      661 ccgaagcgca ggaggcagca cgggagacca tccgcaagca catccacgcg gagcacgtgc
      721 acgagatcat cttcacgagt ggcacaacgg cgagcatcaa cctggccgcg gcaagccttg
      781 gtcaactgct cctgaagaag ggcgacgagg tggtgatcag cgggatggag catcacagca
      841 acatcgtgcc ctggcaactg gcatgcgaac ggcacggagc gatgctgaag gtgatcccga
      901 tcacggataa cggggagtgg gatcttggcg cgattccggc ccaaggcccg aatgacaacg
      961 ctcttctttc agagaggaca cgcatcctcg cgatctcgca cgtcagcaac acaatgggca
     1021 cgatcaaccc ggtgaagaag ctcatcgcac aggcgaagaa gcaggggatc acaaccgtgg
     1081 tggatggcgc gcaggcgatg ccgcacctta acatcgatgt gcaggacctc ggttgcgacc
     1141 tctacgcttt cagcgggcac aaggcgtacg gacctaccgg gaccggcgtg ttgtacggcc
     1201 gcgaagaact gatggggtgc atgccgcctt ggcaaggtgg cggcgagatg atcgataccg
     1261 ttacgctgga gaagagcact tgggcgaagt tgccgttcaa gttcgaggcc ggcacgcccc
     1321 acatcgccgg catcatcggt ctcggcgagg ccgtccgctg gatggaggac tacgacaagt
     1381 cagcgatggc cgtgcatgaa caccaactga tcgagcacgc gacaaaggag ctgctcgcga
     1441 tcgaaggcct gcgcatcatc ggaacggcga aggagaaagc gggcgtgatc agcttcgtca
     1501 tcgagggtgt gcatcattac gacctcggca ccctgcttga ccaacaaggt atcgccgtac
     1561 gcacgggcca ccattgcacg cagccgctga tggaccgctt cggcgtgagc ggaacgaccc
     1621 gggcgagctt cgcgtgcttc aatacgatcg cggaagtgga tgcccttgta gccggggtaa
     1681 agaaggcggt gaagatgttg cgatgagata ggtgaaagtg gaaatagtgg tgaaaaagtg
     1741 aaatacaagt aacccgtgat catggagaac atgacaaacg atccagtgaa gcgcgactgg
     1801 aacatgaacg ggctctatga tcttggggaa acgatctccg tggtggcaga acaagatggc
     1861 cgcaaggtga acaacttcaa ggatctttgg gtgtggaaga ccggcatgga actgctgaag
     1921 ccggtctatc aggtcacacg taagctcccc aaggaggaac tctatggatt gacgtctcag
     1981 ttacgtcggg ctgctgtatc cgtgccgagc aacattgccg aagggtgggg tcggaacaag
     2041 aagggatatc tactgcttgg cctgagctac tcgcggggct ctcttcatga gatcgagacg
     2101 caactcctcg cctgcgttga gctggaattc ctgaagcatg aggacattca acccctgctg
     2161 gacaagatcg ccggaataag caccggactt ctccgattca tgaataacct ggagtcgaag
     2221 cgctgaggcc actttctcac cattctcacc attctcacca ccctcaccat tctcaccacc
     2281 ctcaccgtgt ccctcgccca aacccaacaa gacctcgttg aggagttcgc cctgttcacg
     2341 gattggcagg accgctacga acacctgatc gaattgggga aggaccttcc gttgatcgca
     2401 ccagagaaca aggtggatgc gaacctggta cgcggttgcc aatcgcgcgt gtggctccat
     2461 gcggaactga agaacggcgt cgtgcatctc accgccgaca gcgatgccat gatcaccaaa
     2521 ggcattgtgg ccctgctcgt tcgcgtgtac aacgaccgta cgccgcaaga gatcctcggg
     2581 gcctccacgg acttcatcga ccggatcggc ctgcgcgaac acctgagtcc caaccgggcg
     2641 aacggcctga gcgcgatgat cgaccagatg aagcgctacg cacttgctta ctcctccaac
     2701 tcatgacacc cgaggacaag caccagttgg aggagagcat catcaatgcg ctcaagtcgg
     2761 tgtatgatcc ggagatcccc gtggacatct acgagctggg gctgatctac gatgttgtgg
     2821 tccacgacga tgccagtgtg tacatcaaga tgaccctgac cagccctgcg tgccccgtgg
     2881 ccggttcact gcccggtgag gtggaacaga aggcggccgg tgtacaaggg gtgaagagcg
     2941 ccaaggtgga actcaccttc gagccgcctt gggaccgtac catgatcagt gaagcggcac
     3001 agttggagct ggggttcatg tgataccctc ccccggatag gggaggcagg accggggtgt
     3061 ggctacctac atttgatgga aagttccctg cctatgctga cacgctacgc ttccagcacc
     3121 ttcgcgctcc tatctgtgtt gtcgagctcg gcacagctga gcatcctcac ccagttcgat
     3181 ccgtccaaca ccgcgggcac ctgcggcatc gcgtacaacg caccggacgg cgagatcatg
     3241 gtgatcgggt gctccgcgag tgtgatagac cggtataccg aggccgggac cttcctttcc
     3301 tcggcgaatg tcgttggtga aggtgccaat gacgtggaca tcgatatggc gccggcggac
     3361 ctggtgatga acggcacctt cgtcgcgcaa ggccagttgt tgttggtgaa cggagaagtc
     3421 ggcgttgcag aggtgtacgc gatcgacgag gtgaccaacg aggtgatcga tacgcttgtg
     3481 agcggcttcg gtgtgagcca cgtggtgggc ggctcgtacc attcgcaacg gggcacgttc
     3541 ttcctggtgc aggacatcca gcctggcggg ttcgaccgca accggatcgc ggagatcgat
     3601 gccaccacgg gcgataccct ccgcacgttc tccttggatc cctatttcga tgtgaactat
     3661 ggcgacctgg atatttccca ggtctcaggc aacctttttc tcgtgagcag cgaagagagc
     3721 gggatcgcgg agttcacccc cgatggcatg ttcattgccg aacatccgct gccctccgga
     3781 gtgtccggcc tcagcggtat cgcgctggac aatgaccttg agggggcgtg ggtgtgcaat
     3841 accagcggcg tagtatataa actggggaat ttccccatgg ggacgttcgg gaacatcggg
     3901 gccccggacc ttcacgttca cccgaatcca acaagcgatg tgctgttggt gacatgtgac
     3961 cgcgcgagca ccaacgacgt tatcgcgatc gtcgatgcgg caggaaggac cctgttgcgg
     4021 gaacgcatca cgaacgcgct catgcggttg gatgtctccc cgctcccggc cgggccgtac
     4081 atcatgcgac tcgaagcgag cggaaagagc cgtcgcttca tgaaggactg aacggatcac
     4141 tccccgccct tctccttctt ctgcggctga agcgggttgg cgctcattcg cttccaccgc
     4201 tcacggttcc ggatgatgtc cagcccgagc cacacggcgt gtcgaaggct gccggggtcc
     4261 gcctcgttcc tgccggcgat gcccagtccg gtaccgtgat ccggtgatgt acgcacgatg
     4321 ggcagtccgg ccgtatagtt cacgccatgt ccgaagctga gggccttgaa cggtgcgagc
     4381 ccctggtcgt ggtacatggc gagcacgcca tcgaagtgct tgtaactgcc gttgccgaag
     4441 aaaccatcgg cggggtatgg acccatggcg ttgatgcctt cctcctgcag cttgcgcacg
     4501 gcgggcgcga tccgttcctt gtcctcactg ccgatagcgc caccatcacc cgcgtggggg
     4561 ttcaggccga gcacggcgat gcgaggctga ttgataccga aatccgactg caacgacctg
     4621 ttgaacaacc gggctttgcc gatgatgcgt tcggtggtca cggcctcggc cacctgattg
     4681 atcggcaggt gaccggtgac cagtgccaca cgcagcccat cgcccaccag gatcatgagc
     4741 acgggcgaat cgctacccgc aagatgggaa aggaactcgg tgtgacccgg gaactggaag
     4801 ccggccagct gcatgctgtg cttgtcgatc ggagcggtca ccagcatgtc gatcttgccc
     4861 tggccgaggt cctgcgcagc ggcttcaagg ctttggatgg cgtacttgct ctgctgcccg
     4921 ctgggcttgc ccagctcgat gggcacatcc tcgtcccaca gatccaccac gttgaatttg
     4981 cgtggtatga tatcgttcac gcttttgacc ccgtggatct gcatgtcatc cagcttcaat
     5041 gccttgcgat ggtggctcac ggctttcaca ccgcagtaca gcacgggcgt gatgtcctgg
     5101 tgcatccggt tgtcctcaaa ggccttcagc accacttcga ggccgacacc gttgaggtcg
     5161 ccgcaactga tgcccactct catcgttttc tcgctcattc tttcctggtg cggggcgccg
     5221 agccccggtg tgttgcccgc ccccctctac cgcccggtgg cgcggcgttt gatgaattcg
     5281 tagaacagcc gtaccccgac accggtgcca cctttggtgc gatattgatc ccgcttgttg
     5341 atgaaggcag gtcctgcgat gtccaggtgg atgaaag
//
LOCUS       DAOQOC010000046         5374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6191_length_5374_cov_4.033277, whole genome shotgun sequence.
ACCESSION   DAOQOC010000046 DAOQOC010000000
VERSION     DAOQOC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6191_length_5374_cov_4.033277"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1509)
                     /locus_tag="PL106_01320"
     CDS             complement(94..1509)
                     /locus_tag="PL106_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNA31734.1"
                     /translation="MKTRYIDLIEQTFDFPKDEFKLVNEQLTWNDLPLMRIIEKHGTP
                     LRITYLPKIGEKIDQARACFAKAFREHGYQGSYEYFYCTKSNHFSYVIDQVTAKGVSL
                     ETSSAYDIGIIELLLERGKLGRDATILCNGYKDERYIRNIGRLKNSGINVIPIIDNLN
                     ELAQLDEVIKGPCDIGIRIAAEEAPKFEFYTSRLGVGYKDIIPFYMRNISKQRKFRLK
                     LMHFFINTGITDSAYYWNELGKCVNVYCDLKKLCPTLDTLDIGGGLPTANSLTYSFDV
                     DYMITEITGRIKDICDENGVHEPNLFSEFGSFTVADSGATFFSIHGQKKQNEKERWNM
                     IDGSFMTTLPDTWAINKRFIMLPINNWNDEYERVFLGGMTCDSDDYYNSEQHSNAIYL
                     PKYRPEQKQYLGFFNTGAYQDSLGGFGGIQHCLIPKPKHVLIDRLPDGTLSDRVFAEV
                     QSAERMMELLGYLPQEELVRK"
     gene            complement(2491..3858)
                     /locus_tag="PL106_01325"
     CDS             complement(2491..3858)
                     /locus_tag="PL106_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA31735.1"
                     /translation="MSIRNRLALQFLLLASLILGAAFLMVYALSADHRKDEFAMRLRE
                     RGTGTAKLLLEVDAVNEDLLTSIEDNNPVGLPGESIGIHDDHGRAIFHLGAPAANELD
                     TALTRRVLSAGLVRDKQGLYESVAFLFPNKGERYIVVVSAVDLYGRSKLRTEIRLMSF
                     TFLLAQVLIFFVGRFYAQRALSPVQRLVSEIKTTSAANLSRRVHAGNGQDEMAQLARS
                     FNDLLDRLDTTFRSQRDFIANASHELRTPLTMISGQLEVLLLKERSSAEYAGTLRSVL
                     EDMHSLNRLSDRLLQMAQAENTAPVSSFSSVRMDEVLWQARSEVIRMDPSNQVEIDLG
                     EVEDEEDLVVHGNETLLRSLVSNLMENACKYSPDRTARVTLKADANELHITVADKGIG
                     ITGEHQARVFEPFFRVDNTATTRGHGVGLSLVARIAQLHKGHVTLVSGPGKGSAFTAH
                     LPKVA"
     gene            complement(3855..4535)
                     /locus_tag="PL106_01330"
     CDS             complement(3855..4535)
                     /locus_tag="PL106_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA31736.1"
                     /translation="MRILVVEDEPKVAAFLKQGLEESGYAVISAHDGPQGRDLALHEA
                     PDLVMLDVVMPGMNGVEVCRAIRQGGGRMPILMLTALGTTDDKVLGLDAGADDYLVKP
                     FEFKELLARVRSLTRRGQQEADSEAPLEYHGLLLDTQRKEAVRGGQRIPLTAKEFALL
                     EFLLRNPGKVLSRALISERVWDIRFDTGTNVVDSYIKFLRKKVDRDFEPKLIHTRVGL
                     GYILTAEP"
     gene            complement(4706..>5374)
                     /locus_tag="PL106_01335"
     CDS             complement(4706..>5374)
                     /locus_tag="PL106_01335"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNA31737.1"
                     /translation="EALRDLGVPAELTGRNDLVVDGRKVSGNAQAVRGDRMLSHGTLL
                     FRSDLDNVVRALRPKPGKLESKGIKSIRSRVVNIGDFLPDTMGMEEFRAALLKHLFPG
                     HAIPPHVKLSDADKAGVLALADSRYRSWDWNFGHSPTFNVQRVERWPIGEVDARLHVE
                     HGHVVHAKFYGDFFSRRDISDLENALAGIRYHPEDLRAAAAAIKADEILEGVGLDELV
                     RLLY"
BASE COUNT          936 a         1559 c         1793 g         1086 t
ORIGIN      
        1 ggagcgagga acgagcgagg acctggacgg aagagtgcga cggcagcgcc ggagcgcggg
       61 ccggggttgt cggcacgccc aaaagaagaa acgctacttc cgcacgagtt cctcctgggg
      121 caggtacccg agcagttcca tcatgcgttc ggcgctctgc acctcggcga acacgcggtc
      181 ggagagggtg ccgtcgggca gtcgatcgat gagcacgtgc ttgggcttgg ggatcaggca
      241 atgttgtatg ccgccgaagc cgccaaggga gtcctggtag gcgccggtgt tgaagaagcc
      301 gaggtactgt ttctgctccg gccggtactt gggcaggtag atggcgttgg agtgctgctc
      361 gctgttgtag tagtcgtcgc tgtcgcaggt catgccgccc aggaagacgc gctcgtactc
      421 gtcgttccag ttgttgatgg gcagcatgat gaatcgcttg ttgatggccc aggtgtcggg
      481 cagggtggtc atgaagctgc cgtcgatcat gttccagcgt tccttctcgt tctgcttctt
      541 ctggccgtgg atgctgaaga aggtggcgcc gctgtcggcc acggtgaagc tgccgaactc
      601 actgaagagg ttgggctcgt gcacgccgtt ctcgtcgcag atgtccttga tgcgccctgt
      661 gatctcggtg atcatgtaat ccacatcgaa ggaataggtg aggctgttgg ccgtgggcag
      721 gccgcctccg atgtccagcg tatcgagcgt ggggcagagt ttcttcagat cgcagtacac
      781 gttgacgcac ttgcccagct cgttccagta gtaggcgctg tcggtgatac cggtattgat
      841 gaagaaatgc atcaacttca ggcggaactt gcgctgcttg ctgatgttgc gcatgtagaa
      901 ggggatgatg tccttgtagc caacgccgag gcggctggtg tagaactcga acttgggggc
      961 ttcctcggcg gcgatgcgga taccgatgtc gcagggcccc ttgatcacct cgtcgagctg
     1021 ggcgagttcg ttcaggttat cgatgatggg gatcacgttg atcccgctgt tcttgagccg
     1081 accgatgttg cggatgtagc gttcgtcctt gtacccgttg cagaggatgg tggcgtcgcg
     1141 cccgagtttg ccgcgctcga gcaggagttc gatgatcccg atgtcatacg cgctgctggt
     1201 ctccaggctg acccccttgg cggtgacctg gtcgatgaca taactgaaat gattgctctt
     1261 ggtgcagtag aaatactcgt agctgccctg gtagccgtgc tcgcggaagg ccttggcgaa
     1321 gcacgcccgt gcctggtcga tcttctcgcc gatcttgggc aggtaggtga tccgcagcgg
     1381 ggtgccgtgc ttctcgatga tccgcatcag gggcaggtcg ttccaggtga gttgttcatt
     1441 caccagcttg aactcgtcct tcgggaaatc gaaggtctgc tcgatcaggt cgatgtagcg
     1501 ggttttcatg tacgggcgtg ccggggggat gggatcgggc gggcagaacg ccgcacgcag
     1561 caggcagcag gcggagcgcc aagtgcgtct ggcggccaac cgcctgctgc ttgctgcgtg
     1621 ctgtgcgagg tcgggaagtg tcggtgcgcg tgggcgagtt cgggtcgccg gttgccgggg
     1681 ttcacgcgcg cgtccatcag cggaaagcgg agggaacgcg ccatgggcca cctacggcga
     1741 tgagcagggc cgaccaggtc tggtcaattc ccgggtgcgg gccacgggag ccgatgctgc
     1801 gtatgcggtt catttcgctt ggcgcaaatg taggggtgcg ggtgatccct gtacgcggcg
     1861 gatccttgtg accgatcgcc gcgtaacgct ccacctggtg aggtggcatg gcgcgggccc
     1921 gcggaacggg acgcgatgcc gtgcgggacc aagttgcgac ctggatggtc ccgtcatgcc
     1981 gctaacggag tataatgcca tttcgcaata cagtatcgtc cgatcggacc atgatatgtc
     2041 acgctgagcc tgtcgacggt tcggcaagct caccgcttcg acaggctcag cgtgacatat
     2101 tggatgacca cgaactgcga attagtataa aataccttac attggatacg gccccgcgtt
     2161 cgctttatgg agcggttcgt tggtcgcagg gaccggtcca accggtacgt cgtgcccatc
     2221 gggtgtacgg catccgggtg atcgacaggt gagaaaggag aaagggaagg aagaccaccg
     2281 ctttgttgga gcggtggtcc ccgcttccct ttcatgctcg gaacacccgg cgcactttca
     2341 ctttcaccat gtgccgggga ctccaggaac tgaccgacct cccccaggtc tttccgcagc
     2401 tggccagaac gctactggtt tgccgcgtgg accgttccgc tgcgaaggtc gggcatgtac
     2461 gaaggggcgg aatgaaaaag ccgtggaaat tcaagcgacc ttgggcaggt gcgctgtgaa
     2521 cgcactgccc ttgcccggcc cggataccag ggtgacgtgt cccttgtgca gttgagcgat
     2581 gcgcgccacc agcgacaggc ccacaccgtg gccgcgcgtg gtggccgtat tgtccacacg
     2641 gaagaaaggt tcgaacacac gcgcctggtg ctcaccggtg atgccgatgc ccttgtcggc
     2701 cacggtgatg tgcagttcgt tcgcgtcggc tttcaaggtg acacgggcag tacggtcagg
     2761 actgtacttg cacgcattct ccatcaggtt gctcaccagc gaacgcagga gtgtctcgtt
     2821 gccatgcacc acgaggtcct cctcatcctc cacctccccg aggtcgatct ccacctggtt
     2881 ggaggggtcc atacggatca cctcgctgcg ggcctgccag agcacttcat ccatgcgcac
     2941 cgaagagaag gaactgaccg gggcggtatt ctcggcctgg gccatttgca gcaggcggtc
     3001 gctgagccga ttcaggctgt gcatatcctc caggaccgag cgcagggtac cggcgtattc
     3061 cgccgatgaa cgttccttga gcaggagcac ttcgagttgg ccactgatca tggtgagcgg
     3121 cgtgcgcagt tcgtggctgg cgttggcgat gaagtcgcgc tgggagcgga aggtggtgtc
     3181 caaccggtcc agcagatcgt tgaacgagcg tgccaactgg gccatctcgt cctggccatt
     3241 gccggcatgc acacgtcggg acaggttggc cgcactggtg gtcttgatct cggacacgag
     3301 tcgctgaacg ggtgaaagcg cacgttgggc atagaagcgc cccacgaaga agatgagcac
     3361 ctgggccagc aggaaggtga aggacatcag gcggatctcg gtgcgcagct tgcttcggcc
     3421 gtacaggtcc acggcggaca ccaccacgat gtagcgctcg cccttgttgg ggaagaggaa
     3481 ggccaccgat tcgtacaggc cctgtttgtc gcgtacgagg cccgcggaga gtacacgccg
     3541 ggtgagcgcg gtgtccagtt cgttcgccgc gggtgcgccc aggtggaaga tggcgcggcc
     3601 gtggtcgtcg tgtatgccga tcgactcgcc gggcaggccc acgggattat tatcctcgat
     3661 gctggtgagc agatcctcgt tcaccgcatc cacctcgagc aggagcttgg cggtgccggt
     3721 acctctttcc cgcaggcgca tggcgaactc gtccttgcgg tggtccgcac tcagggcgta
     3781 caccatcagg aaggcggcgc ccaggatcaa cgaggcgagc agcaggaatt gcagggccag
     3841 ccggttgcgg atgctcatgg ctcggccgtg aggatgtagc ccaggcccac gcgcgtatgg
     3901 atgagcttgg gctcgaaatc gcggtccacc ttcttgcgca ggaatttgat gtagctgtcc
     3961 accacgttgg tgcccgtgtc gaaccggata tcccaaacac gttcgctgat gagcgcgcgc
     4021 gagagcactt tgccgggatt gcgcagcagg aactcaagca gggcgaattc ctttgcggtc
     4081 agtggtatgc gctgtcctcc gcgcacggcc tccttgcgct gggtgtcgag cagcagcccg
     4141 tggtattcca acggtgcttc gctgtcggcc tcctgttgcc cacgccgggt gagcgaccgc
     4201 acccgggcca gcagctcctt gaactcgaac ggtttcacca ggtagtcatc ggcaccggcg
     4261 tccagcccca gcaccttgtc gtcggtggtg cccagcgcgg tgagcatcag gatgggcatg
     4321 cggccacctc cctggcgtat ggcacgacag acctccacgc cgttcatgcc gggcatcacc
     4381 acgtcgagca tcaccaggtc cggtgcttcg tggagggcca ggtcgcggcc ttgtggtccg
     4441 tcgtgcgcgc tgatcaccgc gtagccgctt tcctccaacc cctgcttcag aaaggccgcc
     4501 actttgggtt catcttccac aacaaggatg cgcatcggaa cgtcggggtt gcaacagggc
     4561 gtgcgaaggt atcccggagg agttcggcgc gtacgagccc gttgcaccat ggacggatgc
     4621 acatggaccc ggtggacgtg atgttccttc ggatccggcc attgttgaac actgatattg
     4681 cttcgccgtg ggctttgcgc aggctctaat agagcagcct caccagttca tcgagtccca
     4741 ccccttccag gatctcatca gccttgatcg ctgcggcggc agcgcgcagg tcttccgggt
     4801 ggtatcgtat gccggccagg gcgttctcca ggtcgctgat gtcgcgccga ctgaagaagt
     4861 caccgtagaa tttcgcatgc accacgtggc catgctccac atgcaggcgc gcgtccacct
     4921 cgccgatggg ccagcgctcc acgcgttgca cgttgaaggt gggggagtgg ccgaagttcc
     4981 aatcccagga gcggtacctg gaatcggcca gggccagcac gccggccttg tcggcatcgg
     5041 aaagcttcac gtgcggcggt atggcgtggc ccgggaagag gtgcttcagc agggcggcgc
     5101 ggaattcttc catgcccatg gtatccggaa ggaagtcgcc gatgttcacc acgcggctgc
     5161 ggatggactt gatgcccttg gactccagct tgccgggctt ggggcgcaag gcgcgcacga
     5221 cgttgtccag gtcggagcgg aacagcagcg tgccgtggct gagcatccgg tcgccgcgta
     5281 cggcctgggc gttcccactc accttgcgcc cgtccaccac cagatcgttc cgaccggtca
     5341 attcggctgg tacaccgaga tcgcgcaagg cctc
//
LOCUS       DAOQOC010000047         5366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6201_length_5366_cov_3.343250, whole genome shotgun sequence.
ACCESSION   DAOQOC010000047 DAOQOC010000000
VERSION     DAOQOC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_5366_cov_3.343250"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PL106_01340"
     CDS             complement(<1..633)
                     /locus_tag="PL106_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNA31738.1"
                     /translation="MTQKKRGLGRGLSALLEDSSTDITERIAPSGPPQTRPAGGVANI
                     LIAHIEPNPFQPRTTFSQEALVELAQSIKELGIIQPVTVRKLGYDKYQLISGERRFRA
                     SQLAGLTEVPAYIRIANDEGMLEMALVENIQREELDAIEVAISFQRLVDEVQLTQEQL
                     SEKVGKDRATVANYLRLLKLPPEIQLGLRQRLIGMGHARALITIADPERQI"
     gene            complement(738..1553)
                     /locus_tag="PL106_01345"
     CDS             complement(738..1553)
                     /locus_tag="PL106_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA31739.1"
                     /translation="MAKIIAIVNQKGGVGKTTTAINLAACLGVLERRTLLVDADPQAN
                     ASSGTGFDPREVKASIYESIINGAPAADVVLRTDNPNLDLLPGHIDLVGAEIEMIDMP
                     DREKQMKRVLDPLRDEYDYIIIDCSPSLGLVTVNALTCADSVIVPVQCEYFALEGLGK
                     LLNTIKIVQQRLNPDLLIEGMLLTMYDSRLRLANQVVDEVKTHFQELVFDTVIHRNVA
                     LGEAPSHGQTIIMHDAGSKGAVNYLNLAREILRRNDAPVATTENEGGTLAIAQ"
     gene            complement(1607..2284)
                     /locus_tag="PL106_01350"
     CDS             complement(1607..2284)
                     /locus_tag="PL106_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNA31740.1"
                     /translation="MKVTWYGHSCIGVSIGTHHILIDPFISGNPKAAHIDMGKIPCTH
                     LLVTHGHGDHVGDAVAIAKRTGAVLVSNYEVANWFAQQGVERTVGMNIGGSAEVDGLR
                     VKYTIAHHSSQLPDGAYGGDPGGFVVSTAEGTFHHAGDTALTLDMQLLKRDALKFACL
                     PIGDHFTMGPVDAAEAAVLMGVNKVLGMHYGTFPPIDIDLAAAHRTFQDAGIELFLPA
                     IGTTIDL"
     gene            complement(2741..3136)
                     /locus_tag="PL106_01355"
     CDS             complement(2741..3136)
                     /locus_tag="PL106_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31741.1"
                     /translation="MNTAYQDLYTGLGYLFYSVAASDGRIPKAETGRLKELIERHWLP
                     YENSRDEFGTDAAYYIDIIFDYAVDQELPAEDAYSNFLEVHAEHKALFDDDLKRLVER
                     TASGIAEAMAGVNKSERAVLDRLHRFLQL"
     gene            complement(3197..3802)
                     /locus_tag="PL106_01360"
     CDS             complement(3197..3802)
                     /locus_tag="PL106_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31742.1"
                     /translation="MFRSTGQYLLFAMLPSTSVALSARLPQDLSLMELGDTRILSELM
                     SLGGYQELGRSFAEPNIREIPVSAATEGLVEYRSEGLRARIEAQPFEHADRVEESQGT
                     WRLDGQVLVGWSPGAAHARLSRVEVVLDGAVVDLPTEAYTDVFDAPLLRDGLPYVLAA
                     RSQDGARTYLHLQAGEGTDARLITWVVQNGRYLFRVVDKAP"
     gene            complement(3917..4219)
                     /locus_tag="PL106_01365"
     CDS             complement(3917..4219)
                     /locus_tag="PL106_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HNA31743.1"
                     /translation="MLAYSTLSEAVNDLQRRGYIHDLTAAEKCLICDARGVHLDPAEF
                     EIDEFHRFEGMSDPEDQSIVYAISAKAHGIKGILVNAYGMDASPMTQAMVAKLATH"
     gene            complement(4254..5252)
                     /locus_tag="PL106_01370"
     CDS             complement(4254..5252)
                     /locus_tag="PL106_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNA31744.1"
                     /translation="MSAVTIFWLLLALLVVFIIAKGVRIVQQSEAMVIERLGRYQKTL
                     MAGFNIIIPILDKPREIIFRLTRDLPDGNKYVQFVKKERIDMRETVYDFPRQNVITKD
                     NVMTEINALLYFQVMDPVKAIYEIENLPLAIEKLTQTTLRNVIGELDLDECLTSRDTI
                     NMKLRTILDEASHKWGVKVNRVELQDINPPRDIREAMEKQMRAERDRRAQIIDAEGSK
                     RAAVLQAEGIQQAQITQAEGQKQSQVLEAEGDAQARIRRAQGEAEAISLVTRAVAGSK
                     ADPTTYLIAMKYLETLKEMTSGQGNKVIYIPYEASGVLSSVGSIKEMLEANRTLGQ"
BASE COUNT          906 a         1622 c         1684 g         1154 t
ORIGIN      
        1 gatctgtcgc tccggatcgg ctatggtgat gagcgcgcgc gcgtgcccca taccaatgag
       61 gcgctggcgc aatccgagtt ggatctccgg aggcagcttc agcaggcgga ggtagttggc
      121 caccgttgcg cggtccttgc ccaccttctc gctcaactgc tcctgggtca gttgcacctc
      181 atccaccagg cgctggaaac tgatggccac ctcgatggca tccaactcct cgcgctggat
      241 gttctccacc agtgccattt ccagcatgcc ctcgtcgttg gcgatgcgga tgtacgcggg
      301 cacttcggtg aggccggcga gctgcgaggc ccggaaacga cgctccccgc tgatgagctg
      361 gtatttatcg tacccgagct tgcgtacggt caccggttgg atgatgccca gttccttgat
      421 gctctgcgcc agttcgacca gggcttcctg gctgaaggtg gtgcgcggtt ggaacggatt
      481 gggctcgata tgggcgatga ggatgttggc cacccccccc gcaggtcgcg tctggggcgg
      541 accgctcggg gcgatgcgct cggtgatgtc ggtgctgctg tcctccagca aggcgctgag
      601 gccccggcca aggccgcgtt tcttctgggt catgcgtgtg tggatgtccg caacagggac
      661 agatgatacg ggatcgatgg tagggatctc gggagtgcgc tcttcaagca cgtccgctgt
      721 cattccgata gtgttcctca ttgggcgatg gccagggtac caccttcatt ttcggtagtg
      781 gccaccgggg cgtcgttgcg ccgcaggatc tcgcgggcga ggttcaggta gttcaccgcg
      841 cccttgctgc ccgcgtcgtg catgatgatc gtttgcccgt ggctcggtgc ttcgcccaag
      901 gccacgttcc ggtggatcac cgtgtcgaac accagttcct ggaagtgcgt cttcacctcg
      961 tccaccacct ggttggccag gcgcaggcgg ctgtcgtaca tggtgagcag catgccctcg
     1021 atcaacaggt cggggttcag gcgttgctgt acgatcttga tggtgttgag cagcttgccc
     1081 aagccctcca aggcgaaata ctcgcactgc acgggtacga tcacgctgtc ggcgcaagtc
     1141 aaggcgttca ccgtcaccag tccgagcgag ggcgaacaat cgatgatgat gtagtcgtac
     1201 tcgtcccgca acgggtccag cacgcgcttc atctgcttct cgcggtcggg catgtcgatc
     1261 atctcgatct cggcgcccac caggtcgatg tggcccggca gcaggtccag gttcgggttg
     1321 tcggtgcgca gtaccacgtc ggccgctgga gccccgttga tgatgctctc gtagatgctg
     1381 gctttcacct cgcgcgggtc gaagccggtg ccgctgctcg cgttggcttg cgggtcagcg
     1441 tccaccagca aggtgcggcg ctcaagcacg cccaggcatg ccgccaggtt gatcgcggtg
     1501 gtggtcttgc ccacgccgcc tttctggttc acgatcgcga tgatcttggc cattggtcgt
     1561 tggttgttgg ggtagatgga ccggtggttc ccggccgata gtggtttcag agatcgattg
     1621 tcgtgccgat ggcgggcaag aacagttcaa tgcccgcatc ctggaaggtg cgatgcgcgg
     1681 ccgcgaggtc aatgtcgatg ggagggaagg tgccgtagtg catgcccagt accttgttca
     1741 cccccatcag cacggcagcc tcggccgcgt cgacgggacc catggtgaag tggtcgccga
     1801 tgggcaggca ggcgaacttc agcgcatccc gcttcaacag ttgcatgtcg agcgtgaggg
     1861 ccgtatcccc ggcgtggtgg aacgtacctt cagccgtgct caccacgaag ccgcccggat
     1921 ctccgccgta cgcgccatcg ggcaattgac tgctgtggtg ggcgatggtg tacttcacgc
     1981 gcagcccgtc cacttcggca cttccgccga tgttcatacc gaccgtccgt tccacacctt
     2041 gttgcgcgaa ccagttggcc acctcgtaat tggacaccag aaccgcgcct gtgcgcttgg
     2101 caatggccac cgcatccccc acgtggtccc catgcccgtg ggttaccagc agatgggtac
     2161 aggggatctt gcccatgtcg atgtgcgccg ccttcgggtt cccgctgatg aacgggtcga
     2221 tcaggatgtg gtgcgtcccg atgctcacgc ctatgcagct atggccgtac caagtgacct
     2281 tcatgggttc gttccgctct tgaaaaagac gttggttcaa cgccttttcg acggggccga
     2341 aagtagaagc cgcccttttc cggagggcct tgcacccggg tcagaatttt gaacacgaca
     2401 ctgttccatc gccctgtaac cggtggtttt caacatcgtt gggccatgcc cagcgcgagg
     2461 ttcagcgatt ctcaacaaca tgcgtccgat cgttgataga tcccgtggga cctgcacgtt
     2521 gatgagcccc ccggcaagga caacctggat ggtgttccac ttgtttgcca tcccggttcg
     2581 gcgaaaggtg atgtgatccg gagctggacg cggttcgtac gccctcattc cttggggcca
     2641 ggcttcggca cggcgaaccc ggggaacgga gggcgaaaga gcgggggcct ggtgaattca
     2701 aggtggagcc acgcgcggtg gaccggggtc cgaacgacgg ctacagctgc aggaagcgat
     2761 gcagcctgtc caacacggca cgctcgctct tgttcacccc ggccatggcc tcagcgatcc
     2821 ccgaagccgt ccgctccacc agccgcttca ggtcatcatc gaacaaggcc ttgtgttcgg
     2881 catgtacttc gaggaagttg ctgtatgcgt cctcggccgg tagttcctga tcgaccgcat
     2941 agtcgaagat gatgtcgatg tagtacgcgg cgtccgtgcc gaattcatcc cggctgttct
     3001 cgtagggcaa ccagtgccgc tcgatcagct ctttcaatct cccggtctcc gccttcggga
     3061 tccgtccatc actggcggcc acgctgtaaa agaggtaacc gaggcccgtg tacaggtcct
     3121 ggtaagcggt attcatggag cagaagtacc gctcctccgg gcatgtcgaa atgatggagg
     3181 tcaggtggag ggatggtcaa ggggccttgt ccaccacgcg aaaaagatac cgtccgttct
     3241 gcaccaccca ggtgatcaac ctcgcatccg tgccttcacc ggcttgcagg tgcaggtacg
     3301 tgcgcgcacc gtcttgtgag cgtgccgcca gcacgtaggg aagaccatcg cgaagcagcg
     3361 gtgcgtcaaa gacatccgtg tacgcttcgg taggcaggtc caccaccgca ccgtccagta
     3421 ccacttccac acgactgagc cgtgcatgcg ctgctccggg cgaccatccc acgagcacct
     3481 ggccatcgag ccgccacgtg ccttgacttt cttccacccg gtcggcatgc tcgaagggtt
     3541 gggcctcgat gcgtgcccgt agtccctcgg agcggtattc aacgaggcct tcggtggcag
     3601 ctgagaccgg tatttcccgg atgttcggtt ctgcgaacga ccggcccaac tcctggtagc
     3661 ccccgagcga catgagctcc gagaggatcc gtgtgtcgcc caattccatg agcgacaggt
     3721 cctgcggcaa ccgcgccgag agcgccaccg aggtgcttgg cagcattgcg aacaagagat
     3781 actggccggt cgatcggaac atgcttcggg gatccagcgg ccccgtgttc agctaacgca
     3841 tcgaacgtat cgtgcagtgt gggcggtcac catgccatga tcggtgctga tcgcaccacg
     3901 ggatccgttc gggtgctcag tgcgtggcca gcttggccac catggcctgc gtcatgggcg
     3961 atgcgtccat gccgtatgcg ttcaccagga tgcccttgat gccatgggct ttcgcactga
     4021 tggcgtacac gatgctctgg tcctccggat cgctcatgcc ctcgaagcgg tggaattcgt
     4081 cgatctcgaa ttccgcaggg tccagatgga ccccgcgcgc atcgcagatg aggcatttct
     4141 ccgctgcggt cagatcgtgg atgtagccgc gacgttgaag gtcgttcacg gcttcggaga
     4201 gggtggagta cgcgagcatg gggtgaagat accctccgga ccggcagaat gcgctactgc
     4261 cccagtgtcc tgttcgcctc caacatctcc ttgatgctgc ccacgctgct gagcacaccg
     4321 ctggcctcgt aggggatgta gatcaccttg ttgccctggc cgctggtcat ttccttcaac
     4381 gtttccaggt atttcatggc gatcaggtag gtggtggggt cggctttgct tcccgccacg
     4441 gcgcgcgtga ccagactgat ggcctcggct tcaccctgtg cccgtcggat acgggcttgc
     4501 gcatcgcctt cggcttccag cacctgcgat tgtttctggc cctcggcctg ggtgatctgc
     4561 gcctgctgga tgccctcggc ctgcagcacg gcagcacgct tgctgccctc ggcatcgatg
     4621 atctgcgcac ggcgatcgcg ctcggcacgc atctgcttct ccatcgcctc ccggatgtcg
     4681 cgtggtgggt tgatgtcctg caactccaca cggttcacct tcacgcccca cttgtggctc
     4741 gcttcgtcca ggatcgtacg cagtttcatg ttgatggtgt cccgacttgt gaggcactcg
     4801 tccaggtcca gttccccgat cacgttgcgc aacgtggttt gcgtgagctt ctcaatggcc
     4861 aagggcaggt tctcgatctc gtagatcgcc ttcaccggat ccatgacctg gaagtacagc
     4921 agcgcgttga tctcggtcat cacgttgtcc ttcgtgatca cgttctggcg cgggaaatca
     4981 tacacggtct cgcgcatgtc gatccgctcc ttcttcacga actgcacgta tttgttgcca
     5041 tcgggcagat cgcgggtgag gcggaagatg atctcgcgcg gcttgtccag gatcgggatg
     5101 atgatgttga agcccgccat gagcgttttc tggtagcggc caaggcgctc gatgaccatg
     5161 gcttcgctct gttgcacgat gcgcacacct ttggcgatga tgaagaccac caggagggcg
     5221 agaaggagcc agaaaatggt gactgctgac atgggaggag gggttggtcg gtgttgttca
     5281 gggatggtga agccggtggg tgctgggcat caaggcgcga tcggacgaac gatcagaagg
     5341 agcaatcatg cgacatgacg ggtggg
//
LOCUS       DAOQOC010000048         5332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6269_length_5332_cov_2.821679, whole genome shotgun sequence.
ACCESSION   DAOQOC010000048 DAOQOC010000000
VERSION     DAOQOC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6269_length_5332_cov_2.821679"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2839)
                     /gene="uvrA"
                     /locus_tag="PL106_01375"
     CDS             complement(<1..2839)
                     /gene="uvrA"
                     /locus_tag="PL106_01375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNA31745.1"
                     /translation="MATGVADTTGPDEEVIEVLGARVHNLKNVDVRIPRDKLVVVTGL
                     SGSGKSSLAFDTIHAEGQRRYIETFNAYARQFLGGIERPDVDLITGLSPVIAIEQKTI
                     SRNPRSTVGTITEIYDLLRLLYARAGEAYSHVTGERMVRYTDKRIIDTVIQEYTGREA
                     LILAPLVRGRKGHYRELFEQMLRQGFLRARIDGEVVDLTTRPRLDRYKVHDIEVVVDR
                     VKVDEANRKRIAASCDVALKQGKGNMLVMEAIGPANKTRTGEPRYLSRHLMCPTSGIA
                     YEEPEPNLFSFNSPYGACARCNGLGQVTEVDIARIVPDRSKSLKKGAIVPLGNYKGSW
                     IFLQLEAVLEHFGHTVDTPFAELDEEVFAVLLNGMDDPLKVTSKVGLSDRMVQFEGII
                     PYVLGQAEDGAKGAEKWAEQFTHMVTCPECEGRRLKKLARWFRIGGKDITELARMSIA
                     ELHAHMKALPAHLDEKQALIARDVVKEVADRSGFLLDVGLEYLTLDRGSRTLSGGEAQ
                     RIRLATQIGSQLTGVLYILDEPSIGLHMRDDQRLIGSLRKLRDGGNSVLVVEHDREMM
                     MSADHIIDIGPGAGTHGGHVVAQGTADEMLKAASLTAKYLRGEQRISGPRERRKGNGH
                     RITLKGAGGNNLKQVDAEFPLGTFICVTGVSGSGKSTLIGDTLYPILSRSFHRSEAKA
                     LSYAAIDGLKHIDKVIEVDQSPIGRTPRSNPATYTGLFDHIRELFANLPAAKIRGYKA
                     GRFSFNTAGGRCPTCKGAGVRTIGMNFLPDVDVPCETCRGKRYDRETLEVRFKGKSIA
                     DVLNMPVEEALAHFEDIPQIRMKLRTLMDVGLGYITLGQSSTTLSGGEAQRIKLATEL
                     SRRDTGSTFYILDEPTTGLHFEDVRQLIGVLDRLVDAGNTVLVIEHNMDLIKVADHVI
                     DLGPEGGEGGGRIIAKGTPEEVVLMGRGHTAR"
     gene            complement(2914..3855)
                     /locus_tag="PL106_01380"
     CDS             complement(2914..3855)
                     /locus_tag="PL106_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNA31746.1"
                     /translation="MRILAKIALSVVLLLAGVICVHLFSFSTATDPDLDYQRQFNERY
                     RIFSLSPPEDLSFCGEAVPMNKIDVRERLDRELLVNTYWQSNSVLAHKRAHRWFPVIE
                     PILAREGVPDDLKYIALIESGLTNVISPVGATGYWQFMKETGIAYGLRVDEEVDERYN
                     VVRSTEAACRYLKASKERFGSWAMAAAGYNLGHGGLEKQVGRQSTKDYFNLLLPEETS
                     RYVFRILAMKAIITDPERYGFHLREKDLYPQYNTRSVSITGPIENLNDFAVRQGTDYK
                     TLKLLNPWLRDIKLSSTKGGVYTVLLPGDDFDKPVEE"
     gene            complement(3877..4956)
                     /locus_tag="PL106_01385"
     CDS             complement(3877..4956)
                     /locus_tag="PL106_01385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNA31747.1"
                     /translation="MYSATQLFLIYSGLFFGALVFALLINSVFMRFFRTFGIREGEGG
                     MRWAATQKPAFGGIGFFLIFLGSFAVHGALFPKFDNPYQPELIGLLAATGLGFLMGLA
                     DDAYNTRPVLKFLTQVGCGVILIATGSSIRIFDAQWANWLLTVFWTVGMMNSINMLDN
                     MDGITTVTSISILACGLLSFVPEWKLGTIYPVLVIGVIAALCGFLLFNWHPSRMYMGD
                     TGSQFLGVFLAYIGIRCFWNGLSPSGAVEPWRQAVSTLIVFALPIIDTSTVTVNRLLK
                     GRSPFVGGRDHTTHHLSYLGLSDQQVALVFSGLGAVSFFCVLAMLKFIGVWRAWHSAV
                     FISWWAVMFVALFIITKRAKGPEKG"
     gene            complement(4960..>5332)
                     /gene="rfbC"
                     /locus_tag="PL106_01390"
     CDS             complement(4960..>5332)
                     /gene="rfbC"
                     /locus_tag="PL106_01390"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNA31748.1"
                     /translation="RGLHFQAVPHAQGKLVRVVKGAVLDVCVDIRRGSPTFGQHFKIL
                     LDGEEKVMLWVPEGFAHGFRTLENDTIFSYKCTAPYHPPSERTLLWNDPALAIDWGIA
                     DPVLSAKDRAGLTLAALAPTL"
BASE COUNT          921 a         1743 c         1638 g         1030 t
ORIGIN      
        1 accgggccgt atgcccacgg cccatgagca ccacctcctc gggtgtgccc ttggcaatga
       61 tgcgtccgcc gccctcgccg ccctcgggac ccaggtcgat cacgtggtcc gccactttga
      121 tgaggtccat gttatgctcg atcacgagta cggtattgcc cgcgtccacc agccggtcga
      181 gcacgccgat gagttgccgc acgtcctcga agtgcaggcc ggtggtcggt tcgtccagga
      241 tgtagaaggt gctgcccgtg tcccggcgac tgagttcggt ggccaatttg atgcgctgcg
      301 cctcgccacc gctgagcgtg gtgctgctct gccccagggt gatgtacccc aggccgacat
      361 ccatgagcgt gcgcagcttc atgcggatct gcgggatgtc ctcgaaatgc gccagggcct
      421 cctccaccgg catgttcagc acatccgcga tgcttttgcc cttgaaacgc acctccaggg
      481 tctcacggtc gtaccgtttg ccccggcagg tctcgcacgg cacgtccaca tccggcagga
      541 aattcatacc gatggtgcgc acgccggctc ccttgcacgt tgggcagcga ccaccggcgg
      601 tgttgaagct gaaccgaccc gccttgtacc cgcggatctt cgctgcgggc aggttggcga
      661 acaactcgcg gatgtgatca aagaggcccg tgtacgtggc gggattgctg cgcggggtcc
      721 gaccgatggg gctctggtcc acttcgatca ccttgtcgat gtgcttcaac ccatcaatgg
      781 ccgcgtacga gagcgctttg gcctcgctgc ggtggaagct ccggctgagg atcgggtaga
      841 gcgtgtcgcc gatgagcgtg ctcttgccgc tgccgctcac gccggtgacg cagatgaagg
      901 tgcccagcgg gaactccgcg tccacttgct tgaggttgtt gcctccggcg cccttcagcg
      961 tgatgcgatg accattgccc ttgcggcgct cgcgcggacc gctgatgcgc tgttcgcccc
     1021 tgaggtactt cgccgtgagc gatgcggcct tcagcatctc gtcggccgtg ccctgcgcca
     1081 ccacatgccc accgtgcgtg cccgctccag gtccgatgtc gatgatgtgg tcggcgctca
     1141 tcatcatctc ccgatcatgc tccaccacca gtacgctgtt gcccccgtcg cgcaacttgc
     1201 gcaaactgcc gatcagccgc tggtcgtcgc gcatgtgcag gccgatgctc ggttcgtcca
     1261 ggatgtaaag caccccggtg agctggctgc cgatctgcgt ggccagccgg atccgctgcg
     1321 cctcgccccc gctcaaggtg cgcgatccac gatccaaggt aagatattcc agtccaacat
     1381 ccagcaggaa cccgctgcga tcggccacct ccttcaccac atcgcgcgcg atgagcgcct
     1441 gcttctcatc caggtgagcc ggtagcgcct tcatgtgcgc gtgcagctcg gcgatggaca
     1501 tgcgtgccag ctcggtgatg tccttgccac cgatccggaa ccagcgcgcc agtttcttca
     1561 accgcctgcc ttcgcattcg gggcaggtga ccatgtgcgt gaactgctcg gcccatttct
     1621 ccgcaccctt cgcgccgtcc tcggcctggc ccagcacgta cgggatgatc ccctcgaact
     1681 gcaccatccg gtcgctcagg cccaccttgc tggtcacctt cagcgggtcg tccatgccgt
     1741 tcaacagcac cgcgaatacc tcctcgtcca attccgcgaa cggggtatcc accgtatggc
     1801 cgaagtgctc gagcacggct tccaactgca ggaagatcca ggagcccttg taattgccga
     1861 gcggcacgat cgcgcctttc ttgaggctct tggaccggtc ggggacgatc cgtgcgatgt
     1921 ccacctcggt cacctggccc agtccattgc agcgcgcgca cgcgccgtac gggctgttga
     1981 agctgaagag gttcggttcg ggttcctcgt acgcgatccc cgaggtgggg cacatcagat
     2041 ggcggctcag gtaccgcggc tcgcccgtgc gtgtcttgtt ggccgggccg atggcctcca
     2101 tcaccagcat gttgcccttg ccctgcttga gcgccacgtc gcaactcgct gcgatgcgct
     2161 tgcggttggc ctcgtccacc ttcacccggt ccaccaccac ttcgatgtcg tgtaccttgt
     2221 accggtcgag ccgggggcgc gtggtcaggt cgaccacctc cccatcgatc cgcgcacgca
     2281 ggaagccctg ccgcaacatc tgttcgaaaa gctcccggta gtggcccttg cggccgcgca
     2341 caagcggggc caggatcagc gcctcacgac ccgtatattc ctggatcacg gtatcgatga
     2401 tccgcttatc ggtgtaccgc accatgcgct cgccggtgac gtggctgtac gcttcgcccg
     2461 cgcgggcgta cagcaggcgc aacaggtcgt agatctcggt gatggtgccc accgtgctgc
     2521 gcgggttgcg gctgatggtc ttctgctcga tggcgatcac cggactcagg ccggtgatca
     2581 ggtccacgtc gggccgctcg atgccgccga ggaactggcg cgcgtaagcg ttgaaggtct
     2641 cgatgtaccg acgctggccc tcggcatgga tggtatcaaa tgcaagggag ctttttccac
     2701 ttccgctcag gccggtgacc accaccagct tgtcccgcgg gatgcgcaca tcgacgttct
     2761 tcaggttgtg tacccgcgca cccagcacct cgatcacctc ctcgtccggc cccgtggtgt
     2821 ccgcaacgcc tgtcgccacc ccggtggtcg cgggtccgat ccttttggcg gcccttttcg
     2881 gtgtcggctc tttcgcactt gtccgcttca cactcattcc tcaacgggct tgtcgaaatc
     2941 gtcgcccggc agcaacacgg tgtacactcc gcctttcgtg ctggagagct tgatatcgcg
     3001 cagccagggg ttcagcagct tcaatgtctt gtagtcggtg ccctggcgca ccgcgaagtc
     3061 gttcaggttc tcgatcggcc cggtgatgct caccgagcgc gtgttgtact gcggatacag
     3121 gtccttttcg cgcagatgga agccgtagcg ttcgggatcg gtgatgatcg ccttcatggc
     3181 caggatgcgg aacacgtacc gggaggtctc ctcgggcagc aagaggttga agtaatcctt
     3241 ggtgctctgc cgtcccacct gtttctcgag tcctccatgc ccgaggttgt aaccggctgc
     3301 ggccatggcc cacgatccga agcgttcctt gctcgctttc aggtatcgac aggcggcctc
     3361 cgtgctccgg accacgttgt agcgctcgtc cacctcctca tccacgcgca gcccgtacgc
     3421 gatgccggtc tctttcatga actgccagta tcccgtggcc cccaccgggc tgatcacgtt
     3481 cgtgaggccg ctctcgatca gggcgatgta cttgaggtcg tcgggcacac cctcgcgcgc
     3541 gaggatcggt tcgatcaccg ggaaccaccg gtgcgcgcgt ttgtgcgcca gcacactgtt
     3601 gctctgccag tacgtgttca ccagcagttc acggtccagg cgttcccgca catcgatctt
     3661 gttcatcggt accgcttcgc cgcagaaact cagatcctcc ggcggactga ggctgaagat
     3721 gcggtaacgc tcgttgaact ggcgctgata gtccagatcg ggatcggtgg ccgtgctgaa
     3781 gctgaacaga tgcacgcaga tcacccccgc gagcaggagg acgacggaga gggcgatctt
     3841 ggccaggatc cgcatgccgc gaagatcggg agaagttcac cccttctccg ggcccttcgc
     3901 acgttttgtg atgatgaaca gggcgacgaa catcaccgcc caccagctga tgaagaccgc
     3961 ggaatgccat gcgcgccaca cgccgatgaa cttcagcatg gccaacacgc agaaaaagga
     4021 caccgctccg agcccgctga aaacgagcgc cacctgctgg tcgctcagcc ccagataact
     4081 gaggtggtgg gtggtgtggt cacggccgcc cacgaagggg gaacgcccct tgagcaggcg
     4141 gttcaccgtt accgtgctgg tgtcgatgat gggcagggcg aagacgatca gggtgcttac
     4201 cgcctgccgc cagggctcca cggcacccga tggactcaac ccgttccaga aacaacggat
     4261 gccgatgtag gcgagaaaga cccccaggaa ctggctgccg gtgtcgccca tgtacatccg
     4321 gctcgggtgc cagttgaaca agaggaagcc gcagagcgcc gcgatcacac cgatgacgag
     4381 caccggataa atggtgccca gtttccactc cggaacaaag ctgagcagtc cacaagcgag
     4441 gatggagatg ctcgttaccg tggtgatgcc gtccatgttg tcgagcatgt tgatggagtt
     4501 catcatgccc acggtccaga agaccgtgag cagccagttg gcccattgcg catcgaagat
     4561 ccggatgctg gaacccgtgg cgatgaggat gacgccacaa ccgacctggg tgaggaactt
     4621 caacaccggc cgggtgttgt acgcgtcgtc ggccaggccc atcaggaagc ccagtccggt
     4681 ggcggccaac agaccgatga gctccggctg gtacgggttg tcgaacttcg ggaagagcgc
     4741 gccgtgaacg gcgaatgacc cgaggaagat caggaagaag ccgatgcctc cgaacgcggg
     4801 tttctgcgtg gccgcccagc gcatgccccc ctccccctcg cgtatgccga aggtgcggaa
     4861 gaagcgcatg aagacgctgt tgatgagcag tgcgaagacc aacgcaccga agaacaggcc
     4921 gctgtagatc aggaaaagct gcgtggcgct gtacatcggt cagagggtgg gggccaacgc
     4981 tgcaagggtc agcccggcgc ggtccttggc actgagcacg gggtcggcga tgccccagtc
     5041 aatggcgagt gccgggtcgt tccacagaag ggtgcgctcg gacggcggat gatagggcgc
     5101 ggtgcatttg tagctgaaga tggtatcatt ctcaagcgtg cggaacccgt gcgcgaaccc
     5161 ctcggggacc catagcatca ccttctcctc cccgtccaac aggatcttga agtgctgccc
     5221 gaaggtgggg ctgccacggc ggatgtccac gcacacatcg agtacggcgc ccttgaccac
     5281 gcgcaccaat ttgccctgcg cgtgcggaac cgcctggaag tgcaatccgc gc
//
LOCUS       DAOQOC010000049         5281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6364_length_5281_cov_3.247991, whole genome shotgun sequence.
ACCESSION   DAOQOC010000049 DAOQOC010000000
VERSION     DAOQOC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_5281_cov_3.247991"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2731)
                     /locus_tag="PL106_01395"
     CDS             complement(158..2731)
                     /locus_tag="PL106_01395"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNA31749.1"
                     /translation="MYVVKRDGRREAVKFDKITARVKKLCYGLDPLVDATAMTMKVIE
                     GIYDGVTTTELDNLAAEVAATMTVRHPDYAQLASRIAVSNLHKNTRKSFSETMRDLYM
                     YVDPKTGERASLIAEDVWAIITENAETLDSAIIYDRDFTYDYFGFKTLERSYLLKLDG
                     KVVERPQHLLMRVAVGIHKADIASAIATYNDLSEGWYTHATPTLFNAGTPKPQMSSCF
                     LLQLKEDSITGIYDTLKQCAQISQSAGGIGLSVHNLRAKGSYIKGTNGTSNGIVPMLR
                     VFNDTARYVDQGGGKRKGSFAVYLEPWHDDIEDFLELKKNHGKEEMRARDLFYALWIP
                     DLFMKRVEANGDWTLMCPNECPGLSDTWGEKFEAPYEGYEAAGKGRRTIKAQELWFRI
                     LESQIETGTPYMLFKDAANGKSNQQNLGTIKSSNLCTEIIEYTSADEVAVCNLGSIAL
                     PKFVEKGRFDHEKLFEVTLRLARNLNRVIDHNYYPIPEARRSNMRHRPIGIGVQGLAD
                     AFILMRYPFDSVEAKVLNREIFETIYHAALTASKDLAREEGPYETYPGSPVSKGILQF
                     DMWGVQPSDRWEWDILRDEIKQHGVRNSLLLAPMPTASTAQILGNNECFEPYTSNLYT
                     RRTLSGEFIVVNKYLLRDLVKLGLWSEELKNRIIAANGSVQNIPEIPQNLKDLYKTAW
                     EISQRTILDMAADRGAYICQSQSLNIFMENVNFGKLTSMHFYAWKAGLKTGMYYLRTK
                     AATDAIKFTLDKSKLEAPKEDAASVTLSPPKGTERVPVGSTVTLDPVASGPVVTAASG
                     ATTARTGRVESNEVPDVAVDRQNAERVAALRAQQESREKAQAQISCSLDDPEGCEMCS
                     G"
     gene            complement(3008..3988)
                     /locus_tag="PL106_01400"
     CDS             complement(3008..3988)
                     /locus_tag="PL106_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HNA31750.1"
                     /translation="MEAANEPLLKENKDRFVIFPIQHNDIWSFYKKHEASFWTAEEID
                     LHADLADWSGKLNDDERYFIKHVLAFFAASDGIVNENLAENFLHEVQYPEARFFYGFQ
                     IAMENIHSETYSLLIDTYIKDPIEKDRLLHAIDTVECVKKKAEWALRWIGKGSFAERL
                     IAFAAVEGIFFSGSFCSIFWLKKRGLMPGLSFSNELISRDEGLHCDFACLLYTRHLLN
                     KLPKKTVERIITDAVTIEKEFVTDALPVNLIGMNAKLMQQYIEFVADRLLVELGNEKV
                     YHATNPFDFMDMISLQGKTNFFEKKVGEYQKAGVMTQNKETNKFSLDADF"
     gene            complement(4582..5196)
                     /locus_tag="PL106_01405"
     CDS             complement(4582..5196)
                     /locus_tag="PL106_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNA31751.1"
                     /translation="MIEHRGTLISEDLFEKRFVCDLSACKGACCEQGDSGAPITSEEA
                     AGIEKHYEAIKPYMTARGKKAVEKQGYSVVDMDGELVTPLVAEYQECAFAKKDNQGIW
                     KCGIEQAYRNGEAPVHKPLSCHLYPIRVTRTKYHDALNYDTWDVCKPACSCGAKLDVP
                     VFRFLKDPLIRAYGKEWYDELEIIYQEWISQPTPVRGGAKRAHR"
BASE COUNT          862 a         1626 c         1593 g         1200 t
ORIGIN      
        1 agtatgacga aacacacttc tcaacattgt ctgatattca atgtcgaaat ggtatcacac
       61 tcaacttccc ggtcaagcac tgttgcggca cgactgtcca tgaacgggaa aggcggcccg
      121 aagaccgccc ttccggatcc catccatcaa cgggcaccta cccgctgcac atctcgcagc
      181 cctccggatc atccagcgag cacgagatct gggcctgcgc cttttccctg gactcctgct
      241 gggcgcgcag cgcggccacg cgttcggcgt tctggcggtc cacggccaca tcgggcacct
      301 cattggactc cacccggccg gtacgagccg ttgttgcccc gctcgctgct gtgaccacgg
      361 gcccactggc cacagggtcg agggtgacgg tgctgccaac gggcacacgc tcggtcccct
      421 tcggcgggct cagggtgaca ctcgcggcat cctctttcgg cgcttcgagc ttgctcttgt
      481 cgagcgtgaa cttgatggcg tcggtagcgg ccttggtgcg caggtagtac atacccgttt
      541 tcaggccggc cttccaggcg tagaagtgca tggaggtgag cttgccgaag ttcacgttct
      601 ccatgaagat gttcaggctc tggctctggc agatgtaggc gccccggtcg gccgccatgt
      661 ccaggatggt cctctggctg atctcccacg ccgtcttgta caggtccttc aggttctgcg
      721 ggatctccgg gatgttctgc acgctgccgt tggcggcgat gatccggttc ttcagctcct
      781 cgctccacag gcccagcttc accaggtcgc gcaacaggta cttgttcacc acgatgaatt
      841 cgccgctgag cgtgcgccgc gtgtagaggt tgctcgtgta cggctcgaag cactcgttgt
      901 tgcccaggat ctgggccgtg ctcgcggtgg gcatgggcgc cagcagcagg ctgttgcgca
      961 cgccgtgctg tttgatctcg tcgcgcagga tgtcccactc ccagcgatca ctcggttgca
     1021 cgccccacat atcgaattgc aggatgccct tgctcaccgg cgagcccggg taggtctcgt
     1081 acggtccttc ctcgcgcgcc aggtccttgc ttgcggtgag ggcagcgtgg tagatggtct
     1141 cgaagatctc gcgattgagc actttggcct ccacgctgtc gaacggatag cgcatgagga
     1201 tgaacgcatc ggccagcccc tgcacgccga tgccgatggg gcggtggcgc atgttgctgc
     1261 ggcgcgcctc tgggatcggg tagtaattgt ggtcgatcac gcggttgagg ttgcgcgcca
     1321 gccgcagggt gacctcgaag agcttctcgt ggtcgaaccg gcccttctcc acgaacttgg
     1381 gcagggcgat ggagcccagg ttgcacacgg ccacttcgtc agcgctggtg tactcgatga
     1441 tctcggtgca gaggttgctg ctcttgatgg tgcccaggtt ctgctggttg ctcttgccgt
     1501 tggcggcgtc cttgaagagc atgtaaggag tgccggtctc gatctggctc tccaggatgc
     1561 ggaaccagag ctcctgcgcc ttgatcgtcc tgcggccctt cccggcggcc tcatatccct
     1621 cgtacggcgc ctcgaacttc tcgccccatg tatcgctcaa gccggggcat tcgttcgggc
     1681 acatcagcgt ccagtcgcca ttggcctcca cgcgtttcat gaagaggtcg gggatccaca
     1741 gcgcgtagaa caggtcgcgg gcgcgcatct cctccttgcc gtggttcttc ttgagctcca
     1801 ggaagtcctc gatgtcgtcg tgccagggct ccaggtacac cgcaaagctg cctttgcgct
     1861 tgccaccgcc ctggtccacg tagcgggcgg tatcattgaa gacgcgcagc atgggcacga
     1921 tgccgttgct ggtgccgttg gtccccttga tgtaggagcc tttcgcacgc aggttgtgta
     1981 cgctgaggcc gatgcccccg gcactctggc tgatctgggc acactgtttc agggtatcat
     2041 agatcccggt gatgctgtcc tctttcaact ggagcaggaa gcagctgctc atctgcggct
     2101 tcggcgttcc tgcgttgaag agcgtgggcg ttgcgtgcgt gtaccagcct tcgctcaggt
     2161 cgttgtaggt ggcgatagcg ctcgcgatat cggccttgtg gatgcccacc gccacgcgca
     2221 tgagcaagtg ctgcggccgc tcgaccacct tgccgtccag cttcagcagg tagctgcgct
     2281 ccaaggtctt gaacccgaag tagtcgtacg tgaaatcgcg gtcgtagatg atggcgctgt
     2341 cgagcgtttc agcgttctcc gtgatgatcg cccacacgtc ctcggcgatc aggctggcgc
     2401 gctcaccggt cttggggtcg acatacatat aaagatccct catcgtttcg ctgaaggact
     2461 tccgggtgtt cttgtgcagg ttgctcaccg ctatgcgact ggccagctgc gcgtaatccg
     2521 ggtggcgcac cgtcatggtg gcggccactt cggcggccag gttgtccagt tcggtggtgg
     2581 tcaccccgtc gtagatgccc tcaatgacct tcatggtcat ggcagtggcg tccaccaagg
     2641 ggtccagccc gtaacacagc ttcttcaccc gggccgtgat cttgtcgaat ttcaccgcct
     2701 cccggcgccc gtcacgtttt actacataca tgctgctcag aaagttgttg tcctggccct
     2761 ccatcgttgg ctcccgccgc ggggaagggt tcggggttta ggggaaaatg ccgatcgcgg
     2821 gcttcggcac ggtggccggg cttcgcggtt cctaaggttc ttttttgagg gggttgttcg
     2881 aggtgaactg ccgatcgatc ttcaaccggg tgatgttcgt tgtgtcggtt ccgggttgcg
     2941 ggttacgagt tgcgggttca gagcggacca acccgcaacc cgcgacaggc caacgaatgc
     3001 cccgcgctca gaagtccgcg tccaaggaga acttgttcgt ttccttgttc tgggtcatca
     3061 ccccggcctt ctggtactcg cccactttct tctcgaagaa gttggtcttg ccttgcagac
     3121 tgatcatgtc catgaagtcg aacgggttgg tggcgtggta caccttttcg ttgcccagtt
     3181 ccaccagcag gcggtcggcc acgaactcga tgtactgctg catgagcttg gcgttcatgc
     3241 cgatcaggtt caccggaaga gcgtcggtga cgaactcctt ctcgatggtc actgcgtcgg
     3301 tgatgatccg ctcaacggtc ttcttgggca gcttgttcag caagtgccgc gtatagagga
     3361 ggcaggcgaa atcgcagtgc aggccctcat cgcggctgat aagctcgtta ctgaagctga
     3421 ggccgggcat cagaccgcgc ttcttgagcc agaagatgct gcaaaagctg ccgctgaaga
     3481 agatgccctc cacggccgcg aaggcgatga ggcgctcggc gaagctgccc ttgccgatcc
     3541 agcgcagggc ccactcggcc tttttcttca cgcattccac cgtgtcgatg gcgtgcagca
     3601 gccggtcctt ctcgatcggg tccttgatgt aggtatcgat cagcaggctg tacgtctcgc
     3661 tgtggatgtt ctccatggcg atctggaagc cgtagaaaaa gcgggcctcg gggtactgca
     3721 cctcgtgcag gaagttctcg gccaggttct cgttcacgat gccgtcactg gcggcgaaga
     3781 atgccaatac atgcttgatg aagtaccgct catcgtcgtt gagcttgccg ctccaatcgg
     3841 ccaggtccgc gtgcaggtcg atctcctcgg ccgtccagaa actggcctcg tgcttcttgt
     3901 agaagctcca gatgtcgttg tgctggatgg ggaagatgac gaaccggtcc ttgttctcct
     3961 tcaggagcgg ttcgttggcg gcctccacct cgcgggagag cacatcctcc aggctggtct
     4021 gggcgttggt cttcgtgggc tgcatgatct gtctgataag ggtgacgagg ttctgaagga
     4081 gggcggcctc ctttttcaat tcgcgatctt tttccgggtc cggtgcgtgc tttctgaagc
     4141 aacgccgacg ggtgacaaat gtggccgacg acggtgctgc gggcaaggcg gaacaggccc
     4201 ggtctttccc atgttttcaa cacgcgctgg tgtgccgcgc ggatcgtggg gttgatcggg
     4261 ccgcgggatt tgcaagcctc ttttgttcac attgcttcaa caacgccgga actccgctcg
     4321 tgacgggcat tcgcacatcc acgcctcgcc aatgccgcga tcaggaagcg atgcggagca
     4381 gtacatgcgc ccgatgtccc ggatgatgcc ctgatcgggc cgtcagttca acgtcagcac
     4441 acgatccgcg ggcggtccga tccatcggcc catgcgaagc gcccggtatt cgcggatccg
     4501 cgacggatcc tggtccatcc tgcccattcc cagcaaggcc cccctgtccg acacgcgctt
     4561 cattgcgcgt ttcatcccgg actatcggtg ggccctcttc gccccgccgc gcaccggggt
     4621 cggctggctg atccactcct gatagatgat ctccagctca tcgtaccatt ccttgccata
     4681 cgcacggatc aacgggtcct tcaaaaaacg gaacaccggc acgtccagct tggcaccgca
     4741 cgagcacgcg ggcttgcaaa cgtcccaggt gtcgtagttc agcgcgtcgt ggtacttcgt
     4801 cctcgttaca cggatgggat agaggtggca actgagtggt ttgtgtaccg gtgcttcacc
     4861 gttgcgatac gcctgctcga taccgcactt ccagatgccc tgattgtcct tcttcgcgaa
     4921 ggcgcactcc tggtattcgg ccaccaacgg cgtcaccaac tcaccatcca tgtccaccac
     4981 gctgtagccc tgcttctcca cggccttctt gccacgtgcg gtcatgtacg gcttgatggc
     5041 ctcgtagtgc ttctcgatgc ccgctgcttc ctccgaggtg atgggggcac cgctatcacc
     5101 ctgctcgcag cacgcgccct tgcacgcgct caggtcgcac acgaagcgct tctcgaaaag
     5161 atcctcggag atgagggtgc cacggtgttc gatcatcggg ggcgaaacta cttggacgct
     5221 acttcaaaaa gccgttcact ctctcggaaa tgcgccgctt cacgtattcc catgtttgtc
     5281 c
//
LOCUS       DAOQOC010000050         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6480_length_5228_cov_2.966944, whole genome shotgun sequence.
ACCESSION   DAOQOC010000050 DAOQOC010000000
VERSION     DAOQOC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_5228_cov_2.966944"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PL106_01410"
     CDS             complement(<1..516)
                     /locus_tag="PL106_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA31752.1"
                     /translation="MPDIHETLRTVWGYDAFRPRQEEIVRAVLAGRDTLALLPTGGGK
                     SLCFQVPALAMRKLCLVVSPLIALMKDQTERLRKLGVRAASITSGMDQHEVENTLNSA
                     GLGKLDFLYVSPERLLTSIFQARLPGLPLGLIAVDEAHCISQWGYDFRPPYLKIAEVR
                     EKHPDVPVLALT"
     gene            658..1728
                     /locus_tag="PL106_01415"
     CDS             658..1728
                     /locus_tag="PL106_01415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNA31753.1"
                     /translation="MITLGQKIDIQPVKHSRLPATDLNQVKFGRVFSDHMFVMDYVDG
                     QWREPRIVEYSDLRMSPAALVLHYAQTIFEGLKAYRSMDGSVKLFRPEMNIKRMNLSA
                     HRMCMPALPEDLFLDALKTLIRMDKGWIPSDDEGALYIRPFMYASDEYIGVKPSDNYR
                     FIIFTCPVRNYYKDTVRVKVETKYSRAFPGGTGEAKCGGNYAASLYPAQLAQREGFEQ
                     LLWTDGEEHAYFEESGTMNVFFRIDDTMLTPATTGSILHGVTRDSIIRIAQHEKVPIE
                     VRRITVKEVVDALKQGRLKAAFGAGTAATIAHIRSIAYEGITYDLPPVEERNLSNHLG
                     QVLDDIRRGRIADPFGWMVPVE"
     gene            1849..2550
                     /gene="cmk"
                     /locus_tag="PL106_01420"
     CDS             1849..2550
                     /gene="cmk"
                     /locus_tag="PL106_01420"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNA31754.1"
                     /translation="MRRITIAIDGFSSCGKSTLAKQLAHHLRYTYIDSGAMYRAVALY
                     AIENGLIVDGVLNLADLLRVLPGIRIGFEHDDSTQRSETWLDGRNVEHRIRELDVSNC
                     VTLVSPVPEVRAKLVRTQQELGAGGGVVMDGRDIGTVVFPGAEVKFFMTARPEVRAER
                     RYKELRGRGADVTFESVLANIGRRDLDDTTRKADPLVQAPGAIVIDNSNMTSAEQFDI
                     ALGHVVKAIGTVAEA"
     gene            2784..3344
                     /gene="msrA"
                     /locus_tag="PL106_01425"
     CDS             2784..3344
                     /gene="msrA"
                     /locus_tag="PL106_01425"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNA31755.1"
                     /translation="MNQEQNTDLSSLDTLVLGAGCFWCTEAVFAELKGVVSVTPGYTG
                     GHTSDPDYKSVCTGGTGHAEVARVVFDTNMVSVDEVLEVFWQTHDPTTLNRQGADVGT
                     QYRSAIFYRNEEQRTKAMHYKAELDKSGAFPRPIITEIVPLTVFWPAENYHQDYYANN
                     PEQGYCQMVIRPKLEKFRKAFAHKLK"
     gene            3399..4145
                     /locus_tag="PL106_01430"
     CDS             3399..4145
                     /locus_tag="PL106_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31756.1"
                     /translation="MTRPCLSTLAVLLTTFSLAQSTGEELVRNGGFEFLDKEPDTYDM
                     IKRAVGWDNVTIGYSEVFSKLAPAKTVGIPMNDYGQMDPKEGDHYAGFFGWKDDEKWN
                     FETGGDPFVAGWSSYSEYITSELMKPLEEGREYEVSFWVALSGNSDRAVSGIGAYFSE
                     DKLNEQHRRFLRQKPQVSVDGFVEKKGEWVEVKGTFEADGGERYITIGTWPSAGFDGK
                     RLIAGPDNKYAYYYVDGISVKALPKEEPAK"
     gene            4220..>5228
                     /locus_tag="PL106_01435"
     CDS             4220..>5228
                     /locus_tag="PL106_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009744597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNA31757.1"
                     /translation="MKKLNLLAATAIASLPAFAQGPNLVKNAGFEELNKPVTTWDQLK
                     RATGWSNANIGTSDVFDKLACYVSVPDNALGTGTPAFEGERYAGFVAYKEDMRQNWKS
                     MFWADEPHRNPGYQQYAEYLQTELSAPLTAGQQYDVSFRVKLSKGSDRAVSGLGGYLS
                     PVALAYKHPHHVNEKPQVSTATVVNDKQNWTEVKGTFTADGTEKFLILGAFPSAGMEK
                     ANVKEGADNQHAYYYIDGISVNIHPEEDRDKDGIIDKEDNCPDIAGLAALKGCPDKDG
                     DGIADALDACPDKAGLAEFKGCPDSDGDGIADNVDRCPNEKGVASMKGCPEINEKTKQ
                     LF"
BASE COUNT         1119 a         1543 c         1640 g          926 t
ORIGIN      
        1 cgtgagcgca agtaccggca catcgggatg cttttcccgt acctcggcga tcttcaggta
       61 gggcggccgg aaatcgtatc cccattggct gatgcagtgc gcctcgtcca ccgcgatgag
      121 tcccaggggc agcccgggaa gtcgagcttg gaagatcgac gtcagcaggc gttccggcga
      181 cacatagagg aagtcgagct tgcccaggcc ggcgctgttc agcgtgttct ccacctcgtg
      241 ctggtccatg ccgctggtga tggacgcggc gcggaccccg agtttccgca gcctctcggt
      301 ctggtccttc atcaacgcga tgagcgggct caccaccagg cacaacttcc gcatggccaa
      361 cgcgggcacc tggaagcaca ggcttttacc gccaccggtg gggagcaggg cgagtgtatc
      421 gcggccggcg agcacggcac gcacgatctc ctcctgccgg ggcctgaacg catcatagcc
      481 ccagacggta cgcagggttt cgtggatgtc gggcatgtgg ccccgcaggt tcgccggtgc
      541 ccaaatatcg gggtgaacag tgccggagtt caggttcgtg cgtggatcgg gatggagaag
      601 cccgcgccca gcacgggaga gcctacattt ggcctcatca cgtaaagcat cgcgatcatg
      661 atcaccctgg gacagaagat cgacatacag cccgtcaaac acagccgcct gcccgcaacg
      721 gacctgaacc aggtgaaatt cgggcgcgtg ttcagtgacc acatgttcgt gatggactac
      781 gtggacggtc aatggcgcga accgcgaatc gtcgaatact cggacttgcg catgagcccg
      841 gccgcgctgg tgctgcacta tgcccaaacg atcttcgagg gactgaaagc ctaccgatcg
      901 atggacggca gcgtcaaact cttccggccg gagatgaaca tcaagcggat gaacctcagc
      961 gcacaccgca tgtgcatgcc ggcgctgccg gaggacctgt tccttgacgc gctgaagacg
     1021 ctgatccgca tggacaaggg atggatccct tcggatgatg agggcgcgct gtacatccgt
     1081 ccgttcatgt acgcgagcga cgagtacatc ggggtgaagc cgagcgataa ctaccgcttc
     1141 atcattttca cctgtccggt acgcaactac tataaggata cggtgcgcgt caaggtcgaa
     1201 accaagtaca gccgggcttt tccgggaggc accggcgagg cgaaatgcgg cggcaactac
     1261 gctgcatcgt tgtaccccgc acaactggcc cagcgcgaag gcttcgaaca attgctgtgg
     1321 accgatggcg aggaacacgc ctacttcgag gagagcggga ccatgaacgt gttcttccgc
     1381 atcgacgata cgatgctcac gccggccacc acgggcagca tcctgcatgg cgtcacccgg
     1441 gacagtatca tccgcatcgc gcagcacgag aaggtgccca tcgaggtacg acggatcacg
     1501 gtgaaggagg tcgttgatgc gctcaagcag gggcgcctga aagcagcgtt cggtgcgggc
     1561 acggcggcca ccatcgcaca catccgcagc atcgcgtacg aggggatcac gtacgacctt
     1621 cctcccgttg aggagcgcaa cctgagcaac catctgggcc aggtgctcga cgacatccgg
     1681 cgtggccgca tcgccgaccc cttcggttgg atggtgccgg tggagtaacg tgtacgatcg
     1741 tgcccacgaa agcgcgattc cgctcccggg tggtcgtgcg cggtacggat cattatcggg
     1801 aaggggatcc atgcgcctga ccggctgagt acgatcttcg ccggatccat gcgccgcatc
     1861 accatcgcca tcgacgggtt ctcctcgtgc ggcaagagca cgttggccaa gcaactggcg
     1921 caccatctcc gctacaccta catcgatagt ggtgccatgt acagggcggt ggcactgtat
     1981 gccatcgaga atggattgat cgtggacggg gtgctcaatc tggcggacct gctgcgggtg
     2041 ctgccgggta tacggatcgg cttcgagcac gacgattcca cgcagcgcag cgagacctgg
     2101 ctcgatggtc gcaacgtgga gcatcggatc cgggaattgg acgtgagcaa ctgtgtaaca
     2161 ctggtcagtc ctgtgcccga agtgcgcgcc aaactcgtgc gcacacagca ggaacttggg
     2221 gcgggtggtg gtgtcgtgat ggacggtcgc gacatcggga ccgtggtatt ccccggtgcg
     2281 gaggtgaagt ttttcatgac agcgcgcccc gaggtacggg ccgagcgtcg gtacaaggaa
     2341 ttgcgcggtc gcggcgcgga cgtcaccttc gaaagcgttc tggccaacat cggccggcgc
     2401 gatctggacg ataccacgcg caaagcggac ccgctcgttc aggccccggg tgccatcgtc
     2461 atcgacaaca gcaacatgac ctcagcggag cagttcgata tcgcgttggg gcatgtggtg
     2521 aaggcgatcg gtaccgtggc cgaggcatga ggcccctcag gcggtacgtt tgtcatgtgg
     2581 ccgccactgc tatgaacatg ctccgatcgg atcatcaggc cgcacctgtc cttcgtggta
     2641 ctgggcaagg ttcccggtcc gatcgggcat ccagcacggc gtgggcaagc tttacgtgtg
     2701 taccgctcac attcgccctc ctggcctgta cctcaccggc cgaacctgcc ggtggaatac
     2761 ccatctccaa cacgacaatt cccatgaacc aggaacagaa cactgacctc tcctcgttgg
     2821 acacccttgt tctcggggcc ggctgtttct ggtgtaccga ggcggttttc gcggagttga
     2881 aaggcgtggt gtcggtaacg cccggatata ctggtgggca taccagcgat ccggattaca
     2941 agagcgtttg cacgggcggc accggccatg ccgaagtggc ccgtgtggtc ttcgacacga
     3001 acatggtgag cgtggatgag gtactggagg tgttctggca aacgcatgac cccaccacat
     3061 tgaaccggca gggtgccgat gtgggcacgc aataccggtc ggccattttc taccggaacg
     3121 aagaacagcg gacgaaggcc atgcactata aggctgaact ggacaagagc ggagcattcc
     3181 cgcgacccat catcacggag atcgtaccgc tcacggtatt ctggccggcc gagaactacc
     3241 atcaggacta ctacgccaat aaccccgagc aaggctattg ccagatggtg atccgcccca
     3301 agctggagaa gttccgcaag gccttcgcac acaagctgaa atagggatgt ggcccgtact
     3361 tttggcaggc ttggcacgaa gcctgctgca cgttccgcat gaccagaccc tgtctctcca
     3421 cgctcgccgt tcttctcacc accttttcct tggcccagtc aaccggcgag gaactggtgc
     3481 gcaatggggg tttcgagttc ctcgacaagg agccggacac ctacgacatg atcaagcgtg
     3541 ccgtgggttg ggacaatgtc accatcgggt actccgaagt gttcagcaag ctggccccgg
     3601 cgaaaacggt gggtatcccg atgaacgact acggccagat ggacccgaag gaaggtgatc
     3661 attacgccgg tttcttcggc tggaaggatg acgaaaaatg gaacttcgag accggcggag
     3721 accccttcgt tgcaggatgg agttcctatt ccgagtacat caccagcgaa ttgatgaagc
     3781 ccttggaaga gggccgcgag tacgaggtct cgttctgggt ggcgctttcg ggcaacagcg
     3841 atcgtgccgt ttcgggcatc ggagcctatt tctcggaaga caagctcaat gagcagcacc
     3901 gccggttcct gcggcagaaa ccacaggtgt ccgtggacgg gttcgttgag aaaaagggtg
     3961 aatgggtgga ggtgaagggc accttcgaag cggacggcgg cgagcgatac atcaccatcg
     4021 gtacctggcc atcggccggc ttcgatggca agcgcttgat cgcaggcccc gacaacaagt
     4081 acgcctacta ttacgtcgat gggatcagtg tgaaagccct tcccaaggag gagcctgcga
     4141 agtaggcggg gagaaggggt gtgaccggac cgttgatcgt ccgtcccaag ggtccaccaa
     4201 cacactgaac tcccacatca tgaagaagtt gaatctgctg gctgccaccg cgatcgccag
     4261 cctccccgcc tttgctcaag gccccaacct ggtgaagaac gccgggttcg aagagctgaa
     4321 caaacccgtt accacctggg accagctcaa gcgggccacc ggatggtcca acgccaacat
     4381 cggcacgagc gacgtattcg acaagctggc ttgttacgtg agtgtgccgg acaatgcgct
     4441 cggcacggga acacccgcct tcgaaggtga acgctacgcg ggcttcgtcg cctacaagga
     4501 ggacatgcgc cagaattgga aaagcatgtt ctgggccgat gagccgcacc gcaaccccgg
     4561 ctatcagcaa tacgcggaat acctgcagac cgagttgagc gccccgctca cggccggtca
     4621 gcaatacgat gtctccttcc gcgtgaagct gagcaagggc agcgatcgcg ccgtgagtgg
     4681 actgggtggc tacctctcac cggtggcact cgcttacaag catccgcatc atgtgaatga
     4741 gaagccgcag gtgagcaccg ccacggtggt gaacgacaag cagaactgga ccgaggtgaa
     4801 gggcaccttc accgccgacg gcacggagaa attcctgatc ctcggggcgt tccccagtgc
     4861 gggcatggag aaagccaacg tgaaggaggg tgcggacaac cagcacgcct actactacat
     4921 cgatgggatc agcgtgaaca tccatcccga ggaggatcgc gacaaggacg ggatcatcga
     4981 caaggaggac aactgcccgg acatcgcagg cctggccgcg ttgaaaggct gtccggacaa
     5041 ggacggtgac ggcatcgccg atgcgttgga tgcctgtccc gacaaggccg gtctggccga
     5101 attcaaaggc tgcccggaca gcgatggcga cggcatcgcc gataacgtgg accgctgccc
     5161 caacgagaag ggcgtggcca gtatgaaagg ctgccccgag atcaacgaga agaccaagca
     5221 gttgttcg
//
LOCUS       DAOQOC010000051         5210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6519_length_5210_cov_4.832202, whole genome shotgun sequence.
ACCESSION   DAOQOC010000051 DAOQOC010000000
VERSION     DAOQOC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6519_length_5210_cov_4.832202"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2263
                     /locus_tag="PL106_01440"
     CDS             <1..2263
                     /locus_tag="PL106_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNA31758.1"
                     /translation="IGGTADRLELFRSDAGTATSTGLQSPDGVVNSSTSNPFRIQVDR
                     TAAAAWTLRFDDGVTGTWASAGTLTETTWTSTGFFGIRIEQSSAGSVVNNHFFDDVSV
                     APIPVDNLPPELLSATIISDIQVDLAFNEAVDPATSEEENNYGIIPFNGATSAVRDAT
                     DHALVHLTLDFPMQSGNAYTITVNGVEDLVGNACSGETVDVLYFVPDVPQPGDVIINE
                     IMADPDPPVGLPNAEFIGIHNATADRTFDLAGWTIADGTGTGMLPAGILPPGAYVILC
                     DDGTAPLFTPFGTTIGFASFPSALTNTGETLTLSAPGGTIIDAVTYSDTWYHDEVKRQ
                     GGWTLERIDPTAPCSGASNWTASTDQQGGTPGTQNSVFAIVPDNTPPAVLSTQVIDAN
                     TIALSFNEAMDVASLPDGTYTITPSIAISAVVVISENSVQLTLAEDLVAGVLYGITVD
                     DVSDCPGNPIGTDNSAGFALPEPVAPGDVVINEVLYDPRGSGSDMVELYNRSQKTLSL
                     AGWKLANVQAGMVADPRIITPDGHLLLPGQYVLVCEDAENIMANYPQSHVDRFVVSDL
                     PSYNNGSGSVVLLAPNDTLLDRTDYNDNLHFALLNSTDGVSLERVNPDRPSNDQTNWH
                     SAAELAGWATPGFQNSQYSVASAAGELSIERAVFSPDNDGYEDVLTIGYRFDAPGFTG
                     TLKIFDVAGREVRTLLNNQLLGTTGAVSWDGITEGGSLARMGPYVVMLEAFAVNGDVE
                     RFRQAVVLAHKLN"
     gene            2345..3655
                     /locus_tag="PL106_01445"
     CDS             2345..3655
                     /locus_tag="PL106_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNA31759.1"
                     /translation="MTEQITRSQELIARRKAAVPNGVGMFNYATAAKAVGATITDEDG
                     RELIDFAGGIGVVNAGHCPPPVVKAIQDQAAKFLHVSFNVASYEPYIALCEKLNQLLP
                     HGGPTKTLLVSTGAEAVENAIKIARQATKRSGVLCFTDAFHGRTLMAMTLTSKVGYKP
                     DCGPFAPEVYRTQFPNYFHHGNGRSEAEFASAELKRLEDLSHNLVDPKSLACVIIELV
                     QGEGGFNVAPKAYVEGLRAWCDKHGILLIFDEIQAGFGRTGAWSAFSHFGVTPDLSTW
                     AKSLGSGMPIAAVMGKAELMDKAGPSSIGGTYIGNPVSCAAALATLQYMEEIDINAKG
                     RHVGDVIRKRFEKMKAKHDSIGDVRGLGAMMAMEFNVKRDPAKPDADTCTKLMNACAA
                     RNLVVITAGTDKNVIRVLSPLVISDELLNRGLDIMEEELDKIRG"
     gene            3665..4630
                     /locus_tag="PL106_01450"
     CDS             3665..4630
                     /locus_tag="PL106_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNA31760.1"
                     /translation="MKPFSIAGIQMRVSASAPNVEAMKLKLDILMNLYPWVDLVMFSE
                     LCAYGPLTANAQPVPGAFEQEMCAMAAKHGIWLLPGSIFEHSRNAAGETRIYNTASVI
                     DPEGSVVARYRKMFPFYPYEVGVTGGDEFCVFDVPEVGRFGVSICYDMWIPETTRTLA
                     VMGAEVILHPTLTGTIDRDIELSIARASAAINQCYFFDINGLDAGGSGKSIVCGPEGR
                     IVYQADVNEEFIPIEINLEKVRRSRELGVLRLGQPMKSFRDRPVEFSVYAPGVKHPYL
                     EGLGPLIKPQRVQELGELQIKDEHRSLDLPKHIVSSFRPSSENNA"
     gene            4672..5178
                     /locus_tag="PL106_01455"
     CDS             4672..5178
                     /locus_tag="PL106_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA31761.1"
                     /translation="MGAPASFNPHSKNKDHIGWFEIPVYDLGRAVQFYGTIRNMRMEI
                     VENGDFAMAYFPTRGGVGGALVQGPGCHPSATGVLIYLNADNEMDDILARVEMAGGRV
                     IMPKTLIGEEAGSFALFIDSEGNRLALHEAPPTPAQPSGPARKAAPKKSTVRKGSAPR
                     KKTARRTR"
BASE COUNT         1083 a         1638 c         1597 g          892 t
ORIGIN      
        1 tatcggtgga accgccgatc gcctggagtt gttccggtcg gacgcgggaa cggcgaccag
       61 caccggttta caaagcccgg atggcgtggt gaacagttcg accagcaacc ccttccggat
      121 ccaggtggac cgcaccgccg ccgccgcctg gaccttgcgt ttcgacgatg gtgtcaccgg
      181 cacctgggcc tcggccggaa cacttaccga aaccacatgg accagcacgg gcttcttcgg
      241 catacgcatc gaacagagca gtgcgggctc ggtggtgaac aaccacttct tcgatgatgt
      301 cagtgtggcc ccgatcccgg tggacaatct tcctccggag ctgctctccg caacgatcat
      361 ctcggacatc caggtggacc tcgccttcaa cgaggccgtg gacccggcca cgtccgaaga
      421 ggagaacaac tacggcatca tccccttcaa tggcgcgacc tccgccgtgc gtgacgccac
      481 cgaccacgca ctcgtacacc tcacgctgga cttccccatg cagagcggca acgcgtacac
      541 cataacggtg aacggcgtgg aagacctggt gggcaacgcc tgctcgggcg agaccgtgga
      601 tgtcctctac ttcgtccctg atgtgccaca accgggcgat gtgatcatca acgagatcat
      661 ggccgatcct gacccaccag taggattgcc gaatgcggag ttcatcggga tccacaatgc
      721 caccgccgac cgcacgttcg atctggcggg atggacgatc gccgacggca cgggcacagg
      781 catgctcccg gcgggcattc tacctcccgg cgcctacgtc atcctgtgcg acgatgggac
      841 cgctccgctt ttcaccccct tcggcacgac catcggcttc gcatcgttcc ccagcgcgct
      901 caccaataca ggtgaaacgc tcacgctttc ggcgcccgga ggtaccatca tcgacgcggt
      961 cacgtattct gacacctggt accacgatga ggtgaagcgg cagggcggat ggacgctgga
     1021 acgtatcgac cccaccgccc cgtgcagcgg cgcatccaac tggacggcca gtaccgatca
     1081 acagggaggc actcctggaa cgcagaacag cgtattcgcg atcgtgccgg acaatacgcc
     1141 tcctgcggta ttgtccaccc aggtcatcga tgccaacacc atcgcgctat cgttcaacga
     1201 agcgatggac gtggcctcac tccccgacgg cacgtacacg atcacaccat ccatcgccat
     1261 cagtgcggtc gtggtcatca gcgaaaacag cgtgcagctc accctcgcgg aggaccttgt
     1321 ggccggcgtg ctgtacggca tcacggtgga cgacgtgagc gattgtcccg gaaaccccat
     1381 cggtacggac aattcggccg gattcgcact gccggagccc gtagcccctg gcgacgtggt
     1441 gatcaacgag gtgctgtacg acccgcgcgg cagtggcagt gatatggtgg aattgtacaa
     1501 tcgctcgcag aagaccctga gcttggccgg atggaaactc gccaacgtgc aagccggtat
     1561 ggtcgccgat ccacggatca tcacacccga tggccacctc ctgctccctg gccagtacgt
     1621 gctcgtgtgc gaagacgcgg agaacatcat ggccaactat ccccaaagcc atgtggaccg
     1681 gttcgtggtg agcgatctgc cgagctacaa caacggcagt ggcagtgtgg tgctgctggc
     1741 ccccaacgac accctgctcg accggaccga ttacaatgac aaccttcatt tcgccctgct
     1801 gaacagcacc gatggtgtga gcttggagcg ggtgaacccg gaccggccca gcaacgacca
     1861 gaccaactgg cacagcgcag ccgaactcgc ggggtgggcc acgcccggtt tccagaactc
     1921 gcaatacagt gttgccagcg cggccgggga gttgagtatc gagcgcgcgg tgttctcccc
     1981 ggacaacgac gggtacgagg acgtgctcac catcggctat cggtttgatg caccgggctt
     2041 tacgggaaca ctcaagatct tcgacgtggc cggacgtgag gtgcgcaccc tgctgaacaa
     2101 ccagctcttg ggcaccactg gggcggtgag ttgggacggc atcaccgaag ggggttccct
     2161 ggcacgcatg ggaccctacg tggtcatgct ggaggccttt gctgtgaacg gcgacgtgga
     2221 gcgcttccgc caggccgtgg tgctggcgca caagctcaat tgagggcccg accctgcacg
     2281 tgttggtatc cttcgccatt cctgaagacc aacggctccg gtattgcgca acttcgcgcc
     2341 cgatatgacc gagcagatca ccaggagcca ggaactgatc gcccgccgca aagccgccgt
     2401 gccgaacggg gtgggcatgt tcaattacgc aacagccgcc aaagccgttg gtgccacgat
     2461 cacggatgag gatggacgcg aactcatcga cttcgcggga ggcatcggcg tggtgaatgc
     2521 gggccattgt ccaccgcccg tggtcaaggc catccaggat caggccgcaa aattcctgca
     2581 cgtgagcttc aacgtggcga gttatgagcc ctacatcgca ctctgcgaga agctgaacca
     2641 gctgttgccg cacggaggac cgacgaaaac actgctggtg agcaccggtg ccgaggcggt
     2701 ggagaacgcg atcaagatcg cgcgccaggc caccaagcgc agtggcgtgc tctgcttcac
     2761 cgacgccttc cacggccgca ccttgatggc catgaccctc accagcaagg tggggtacaa
     2821 gccggactgc gggcctttcg ctccggaggt gtaccgcacg caattcccca actacttcca
     2881 ccatggcaat ggccgcagcg aagcggagtt cgcatcggcg gaattgaagc gcctcgaaga
     2941 cctttcgcac aaccttgttg acccgaagag cctcgcctgc gtgatcatcg agttggtgca
     3001 gggagaaggt ggattcaatg tggccccgaa ggcctacgtg gaagggttgc gcgcctggtg
     3061 cgacaagcat ggcatcctgc tgatcttcga cgagatccaa gcgggcttcg gccgtaccgg
     3121 tgcgtggagc gccttcagcc acttcggcgt aacacccgac ctgagtacct gggccaagag
     3181 cctgggcagc ggcatgccga tcgcggcggt gatgggcaag gcggaactca tggacaaggc
     3241 ggggccgagc agcatcggcg gcacctacat cggcaatcct gtgagttgcg ccgcggcatt
     3301 ggccacgctg caatacatgg aggagatcga catcaacgcg aagggtcggc atgtgggcga
     3361 cgtgatccgc aagcgctttg agaagatgaa ggcgaagcac gacagcatcg gcgatgtgcg
     3421 tggtctgggt gccatgatgg ccatggagtt caatgtgaag cgcgacccgg cgaaacccga
     3481 tgccgacacc tgcaccaaac tgatgaacgc ctgtgccgcg cgcaacctcg tggtgatcac
     3541 agcgggcacg gacaagaacg tgatccgcgt actgagcccc ttggtgatca gtgatgaact
     3601 cctgaacagg ggactcgaca tcatggagga ggagttggac aagatccgcg gttgagcgtt
     3661 caccatgaaa ccattctcga tcgccggtat ccagatgcgc gtcagcgctt cggccccgaa
     3721 cgtggaggcc atgaagctga agctggatat cctcatgaac ctgtacccgt gggtggacct
     3781 ggtgatgttc agcgagttgt gcgcgtacgg accgctcacc gccaacgcgc agcccgtgcc
     3841 cggggccttc gaacaggaaa tgtgcgccat ggccgccaaa cacggcatct ggctcctgcc
     3901 cggcagcatc ttcgaacatt cgcgcaacgc cgcaggagaa acgaggatct ataataccgc
     3961 cagcgtcatc gatcccgaag gcagcgtggt ggcacgctac cgcaagatgt tccctttcta
     4021 cccgtacgag gtgggggtga ccggcgggga tgagttctgt gtgttcgacg ttcccgaggt
     4081 gggccgtttc ggtgtgagca tctgctacga catgtggatc ccggagacca cgcgcacact
     4141 ggccgtgatg ggtgccgagg tgatcctgca cccgacactc accggaacca tcgaccgcga
     4201 tatcgagttg agcatcgcca gggccagtgc ggccatcaac cagtgctact tcttcgacat
     4261 caacggtctt gatgccggcg gcagcggcaa aagcatcgtg tgcggaccgg agggccgcat
     4321 cgtgtaccag gccgatgtga acgaggagtt catccccatc gagatcaatc tggagaaggt
     4381 gcgccgcagt cgcgaactgg gcgtgctgcg cctgggccag cccatgaaga gcttccgcga
     4441 caggcccgtc gaattcagcg tgtacgcgcc aggcgtcaaa catccgtacc tggaagggct
     4501 gggccccttg atcaaaccac agcgcgtgca agagctcggc gaattgcaga tcaaggatga
     4561 gcaccgcagc ctcgacctgc cgaaacatat cgtatcttcc ttccggcctt caagcgagaa
     4621 caacgcataa cgcacaaccg taccgcttcc acaagatcag atcctggcac catgggcgct
     4681 cccgcatcct tcaacccgca ttccaagaac aaggaccata tcggctggtt cgagatcccg
     4741 gtttatgatc tgggccgtgc ggtccagttc tatggtacca tccgcaacat gcggatggag
     4801 atcgtggaga acggcgactt cgccatggcg tatttcccca cccgaggagg tgtcggcggg
     4861 gcattggtcc aaggtcccgg ctgccatccg agtgccacag gcgtgctcat ctacctgaat
     4921 gcggacaacg agatggacga catcctggcc cgtgtggaga tggccggcgg gcgtgtcatc
     4981 atgcccaaga ccctgatcgg cgaagaggcc gggtcgttcg ccctgttcat tgacagtgaa
     5041 gggaaccggc tggccttgca cgaggcccca ccgacccccg ctcagccatc cggaccggcc
     5101 aggaaggccg ccccgaaaaa gtcgacggtg cgcaagggat cggcgccgcg caagaagacg
     5161 gcccggcgca cccggtgatc gcggggtcgc tatcgcacca gcaaacgcac
//
LOCUS       DAOQOC010000052         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6673_length_5134_cov_3.797204, whole genome shotgun sequence.
ACCESSION   DAOQOC010000052 DAOQOC010000000
VERSION     DAOQOC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_5134_cov_3.797204"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2646)
                     /locus_tag="PL106_01460"
     CDS             complement(<1..2646)
                     /locus_tag="PL106_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31762.1"
                     /translation="MKPRSRSRRSLHGVLMATALALATGTNAQLAHTAARSLSHGDHE
                     NDGLEELLHAYDSRLEFAENKGQFSSGVLYRADFPYGQALATPEGMLVKVYDPEAVRA
                     RQEEGMRAEQDLHAGLTGRPLVAREKGHAWRLHFLGALPGMRTGNRDAHSDTRNYFIG
                     GSEALNVRTYQEVWYTGVYRGIDVRYYPAADGSLEYDIICAPGSDPGAIAIEHEGIEQ
                     MRVSDAGELVLMTSLGEMTLPAPVVYQKVGGHEKRIKASYRITGNNVLSFVLGAYDRA
                     LPLIIDPIAMRWATWVNTASSGANHGHCIWVDPNDGAIYVAARPDGSTDQITLGAFDV
                     SANGGVDLVVGKYLEPGSVGGTGTRVWQTYIGGNGQDNPYAMEQGSDGHLYITGLTAS
                     TNFPVLGGSAFTGSSINQQSQSGEDIFVLKLTTDGQSMKAAVVGGNGTDNSFDIRTTS
                     GGDVLVCGSTTSTNLSTLNSGAGASNTNNGGTDAIVFRINQDLSSLTWMRNYGGDGAD
                     RASIMLHDDATGDLFIGGNTASSNFPTVSPRQSTRGGTAAGFLQRLNGNTAAVNWSSY
                     YSSSSGNTNLLCMEFNTTRTELYFGGVTAGLDAANISGGAYDASYNTNNDFYVGRMAI
                     DQTFLGGTYLGGTGEEVNMMGLNVDQNNDVYVFGYTASSNFPVSALPNTPLLGTTQGG
                     QDKVFLKLEYDLGSLLFSTYYGGGQDDTDPVGQRGIKFSNCRIYTIVTARSNNIPLTQ
                     GALNTTKGSSTSVFEPGLVVWANPPDLLGNSITYQGVSICPGSVPGDITGSVPSYVLP
                     TIIRNGTTSSYPSFGNAATYQWQISLDSLNWTDIPGATGQDLSGSMIGAITQDTYIRR
                     VIGGDACILAGAADQVVKVKLMTVGG"
     gene            complement(2686..2841)
                     /locus_tag="PL106_01465"
     CDS             complement(2686..2841)
                     /locus_tag="PL106_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31763.1"
                     /translation="MRKTDPDATRARVHIPVEAGDQQAWHLRSEWADPELWGLCIIGV
                     LLLLATN"
     gene            complement(3290..4756)
                     /locus_tag="PL106_01470"
     CDS             complement(3290..4756)
                     /locus_tag="PL106_01470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA31764.1"
                     /translation="MLRTGPSVFERRWFWFLLVALIVRAVFILWKVSDVRPTTGPGLW
                     YFDTPDTHSYFDPIESLLKGGEYDPDYRMPGVGAPYLLFRLFLGPVGGRDAMVVLQWM
                     LSGLSVLLLTLLGVRLTGSARAGWAVYILFLLSTFSSWYDTSMSSDSLASSILVIQAY
                     LFQRGLDSKDPRLLMAAGFFLGWAVFLRPVAALLFAPALLMLHLYQEKPRTIRMYAIF
                     LLPFALMESYWVIRNWRVNQEFNPLTNQGMMPDEIADRPLGYLMRFLQGYGGDYIWWQ
                     PNADIRWFGLWHGAAEVDDEGRNAPPPPPRAYAPGYTKDSLMTVSALIRSALDGDLTP
                     SDSAAAIAKANAALERYTAIHAASAPWTHHVTSRFLMVRHQVLQNGAETLFARPFSTL
                     PLWMKLFKLLQMILYPFAFLVGSIAAIVSIIKWRGSAPMLQVWLSIMLMYMVLIYPLG
                     LKMAEWRFMVHVFPIALLLATCFTVDRLSIFRGPASDA"
     gene            4923..>5134
                     /locus_tag="PL106_01475"
     CDS             4923..>5134
                     /locus_tag="PL106_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNA31765.1"
                     /translation="MRLATIQTADPRAGYLALRAEIDAAIQDVLNGPGYVLGDAVSRF
                     ETEFARYIGAAHGVGVNNGTDAIHLA"
BASE COUNT          995 a         1549 c         1579 g         1011 t
ORIGIN      
        1 accacccacg gtcatcagct tcactttcac cacctgatcc gccgcaccag caagtatgca
       61 ggcgtcccca ccgatgaccc gacggatata ggtgtcctgg gtgatcgcac cgatcatcga
      121 gccactgaga tcttggccgg tcgcaccagg tatgtccgtc cagttcaggc tgtcgaggct
      181 gatctgccac tgatacgtcg cggcatttcc aaaactcggg tagctggatg tggtgccatt
      241 gcggatgatc gtgggcagca catagctcgg tacggaaccg gtgatgtccc cgggtacgct
      301 tccggggcag atggaaacac cctggtatgt gatgctgttg cccaagaggt cgggagggtt
      361 ggcccatacg acaagaccgg gctcgaacac gctggtggaa ctgcccttgg tcgtgttcaa
      421 cgcgccctgg gtcagcggaa tgttgttcga tcgcgcggtg acgatcgtat agatgcggca
      481 gttgctgaat ttgatgccgc gctggcccac tgggtcggtg tcgtcctgcc cgccgccata
      541 gtaggtgctg aacaggaggg aaccgaggtc atactccagc ttcaggaaga ccttgtcctg
      601 gccaccctgc gtagtgccca gcaacggtgt gttcggcaga gcgctcacgg ggaagttgct
      661 gctggcggtg tagccgaaca cgtacacgtc gttgttctga tccacgttca ggcccatcat
      721 gttcacctcc tcgcccgtgc cgcccaggta ggttccgccc aggaaggtct ggtcgatggc
      781 catgcgaccg acatagaagt cgttgttggt gttgtagctc gcgtcgtacg cgccaccgga
      841 gatgttggcg gcatccaggc cagcggtcac tcccccgaag tataattccg tcctcgtggt
      901 gttgaactcc atgcacaaca ggttcgtgtt gcccgaactg ctcgagtagt aggatgacca
      961 gttcactgct gccgtattgc cgttgaggcg ctgcaggaaa ccggccgcgg taccaccgcg
     1021 cgtgctctgg cgggggctga ccgtggggaa gttggaactg gccgtgttcc ctccgatgaa
     1081 caggtcgccg gtggcgtcat cgtgcagcat gatgctcgca cgatcggcac cgtcaccgcc
     1141 gtagttgcgc atccaggtca gggaactgag gtcctggttg atccggaaaa cgatggcatc
     1201 ggtgccaccg ttgttcgtgt tgctcgcacc cgctccactg ttcaacgtgc tgaggttggt
     1261 gctcgttgta ctgccgcaga ccaggacgtc gccgccgctc gtcgtgcgga tgtcgaagga
     1321 gttgtccgtg ccattgccac cgaccacggc cgccttcatg ctctggccat ccgtggtgag
     1381 cttcagcacg aagatgtcct ctccgctctg cgattgctgg ttgatactgg atccggtgaa
     1441 tgcgcttccg cccagtacgg ggaagttggt gctggctgta aggcccgtga tgtacaggtg
     1501 gccgtcagaa ccctgttcca tggcgtaggg attgtcctgc ccattgccac cgatgtatgt
     1561 ctgccatacg cgcgtgcccg tacctccaac actgcccggt tcaaggtatt tgccgaccac
     1621 caggtccaca cctccgttgg ccgatacgtc gaaggcgccc aaggtgatct ggtccgtgct
     1681 gccgtccggc cgggccgcga cgtagatggc cccatcattc ggatccaccc agatgcagtg
     1741 cccgtgattg gctcctgaac tggcggtatt cacccaggtg gcccagcgca tggcgatggg
     1801 atcgatgatc aagggaagag cccgatcata cgctccgagc acgaacgaca ggacgttgtt
     1861 cccggtgatc cggtaactcg ccttgatgcg cttctcgtga ccgcccactt tctggtaaac
     1921 caccggtgcg ggcagggtca tctcgcccag gcttgtcatg agcaccaact cgcccgcatc
     1981 gctcacgcgc atctgctcga tgccctcgtg ctcgatcgcg atggcgccgg ggtcactgcc
     2041 gggcgcgcaa atgatgtcgt attccagcga gccgtcggcc gccgggtagt accggacatc
     2101 aatgccgcgg tatacgcccg tgtaccagac ctcctggtac gtgcggacat tcagtgcttc
     2161 actacctccg atgaagtagt tgcgcgtgtc gctgtgcgca tcgcggttcc cggtgcgcat
     2221 gccgggcaac gcgcccagga agtgcaggcg ccaggcgtgg cctttctccc gcgctaccag
     2281 cgggcgaccg gtcaacccgg cgtgcagatc ctgctcggcg cgcataccct cctcctgacg
     2341 ggcacgaaca gcctccgggt cgtagacctt caccaacatg ccttccggcg ttgccagggc
     2401 ctgaccgtaa gggaagtcgg ctcggtacag gacaccggaa gagaactggc ccttgttctc
     2461 cgcgaattcg agccgcgagt catacgcgtg caggagttct tccaagccat cgttctcatg
     2521 gtctccatgg gacagggacc gcgccgccgt gtgggcgagc tgcgcattgg tgccggtggc
     2581 caaggcaagc gccgtggcca tcagcacgcc gtggagcgaa cggcgtgagc gtgatcgtgg
     2641 tttcatcggg tgttgaggtt gggagttgag gtatctgaag gttcgtcaat tggttgccag
     2701 taagagcagc acgccgatga tgcagagtcc ccataattcg ggatccgccc attcggatcg
     2761 caggtgccaa gcctgctgat cgcccgcctc caccggtatg tggacacgcg ctcgcgtcgc
     2821 gtccggatcc gttttccgca tgatcatcgt cctgtgctgg tgtccaagca gctctggtcg
     2881 atcctgcggt cccggtcttg cccgggagtg gaaatgtggg caaatgaatg tcgcttgtcc
     2941 ggcggttgtc aagactcgtc gaaagaaagc ggccgtccgt cgatgagatc atgcgccccg
     3001 gctggtatac gaccctggcc ggaacagtct gtttcaacct gcggatcagc gctttttcga
     3061 agctccatgc cacatggaac acgtgagcta cgcatcacac cctgccgatc aatatgaccc
     3121 gcgcaacggc tacctgccct tgccgatgga tcgctgatca cccacgtacc ggatcggtcc
     3181 gaacgatggc gggagcttcc cggatccccg gctcatcgtt catgaggtca ccggcgtgac
     3241 ggtccacttc gctgtcccac gcatggcgcg gagcactttc gaagccggat cacgcgtcgg
     3301 aagctggtcc tcgaaaaatg ctcaaccggt ccacggtgaa gcatgtggcc aacagcaacg
     3361 cgatcgggaa tacgtggacc atgaatcgcc attcagccat tttgagtccc aaaggataaa
     3421 tgagaaccat gtacatcagc atgatggaca gccacacctg gagcatgggt gcggatccgc
     3481 gccatttgat gatcgatacg atcgctgcga tggaaccgac aaggaaagcg aacggatata
     3541 ggatcatctg cagcagcttg aaaagcttca tccagagcgg aagtgtactg aagggccggg
     3601 cgaacagtgt ctccgcacca ttctggagca cctgatgccg caccatgaga aagcggcttg
     3661 ttacatggtg tgtccatggg gcggaagccg catgtatcgc ggtgtaccgc tccagggcgg
     3721 cattggcctt ggcgatcgcc gctgcggaat cggacggggt gagatccccg tccaatgcgg
     3781 aacggatgag cgcactcacg gtcatcagac tgtccttcgt atatccgggg gcataggcac
     3841 gagggggcgg aggcggcgca ttacggccct cgtcatccac ctccgccgca ccatgccata
     3901 gcccgaacca tcggatatcc gcattgggtt gccaccagat gtagtctccg ccataccctt
     3961 gcagaaagcg catcaggtag cccaggggcc ggtccgcgat ctcatcgggc atcataccct
     4021 gattcgtgag cggattgaat tcctggttca cgcgccagtt gcggatcacc cagtagcttt
     4081 ccataagtgc gaacggcaac aggaagatcg cgtacatccg gatcgtccgt ggcttctcct
     4141 gatacaggtg gagcatcagc aatgccggtg cgaacagaag agcggctacc ggcctgagga
     4201 aaacagccca gccgaggaag aacccggcgg ccatcaatag tcggggatcc ttgctgtcga
     4261 ggcctcgttg gaaaagatac gcctggatga cgagtatgga tgacgccaga ctgtccgacg
     4321 acatggaagt gtcgtaccag ctggaaaaag tactgaggag gaacagaatg tacacggccc
     4381 acccggcacg tgcggatccg gtaagacgca cgccgagcag ggtgagcagc agcacactga
     4441 gcccggaaag catccactgc agcacgacca tggcgtcacg accgcccacc ggacccagga
     4501 agagcctgaa cagcaggtag ggggcgccga cccctggcat gcgataatcg ggatcatatt
     4561 cgcctccctt cagaaggctt tcgatcgggt cgaagtagct atgggtgtcc ggggtatcga
     4621 agtaccagag accgggtccg gtggtcggtc gtacgtcgct caccttccag agtatgaaca
     4681 cggcgcggac gatgagtgcg accagcagga accagaacca gcggcgttcg aatacggacg
     4741 ggcctgttcg cagcatgggt tgaccgggcc cgaagttggg agcaagccgg aatgaccatt
     4801 cattgaaaag acgcatcttc tcaaccgtga tctgtcaggc gctccacgat cgcgcgttcg
     4861 tcccggtcag gaatgtggca gtgccccgat cgcccaccgg ctactttcgc cacggaaccc
     4921 gcatgcgcct cgccaccatt cagaccgccg acccgcgcgc aggatacctg gcactccgtg
     4981 ctgagatcga tgcggcgata caggacgtgt tgaacggccc gggatatgtc ctgggtgatg
     5041 cggtctcccg gttcgagacg gagttcgccc gctacatcgg tgcggcccat ggcgtcggtg
     5101 tgaacaatgg caccgacgcc atccatctgg cttt
//
LOCUS       DAOQOC010000053         5119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6704_length_5119_cov_5.845379, whole genome shotgun sequence.
ACCESSION   DAOQOC010000053 DAOQOC010000000
VERSION     DAOQOC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6704_length_5119_cov_5.845379"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PL106_01480"
     CDS             complement(<1..799)
                     /locus_tag="PL106_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA31766.1"
                     /translation="MQAGVLRMEKDGTIREPTATETEVLAVHDQFWQCYARRDLDGRF
                     AVAADDITFFGTALHERAVGKAQYRAFNEKGVEQYPHAFTIEYIWKEVRIWHETAWVE
                     GDTYWTMGSGPEATRDLLRQTTILKRVAGNWLVTHVHGSDPDYRLREGEYMTYGDIHT
                     RNKDLEREVMQRTEELRAEKKRSDALLLNILPEEVAEELKAKGEADAKLIDQVTILFT
                     DFKGFTTISEHMAPRDLVRDLNECFSAFDRICEKHGIEKIKTIGDSYM"
     gene            891..1523
                     /locus_tag="PL106_01485"
     CDS             891..1523
                     /locus_tag="PL106_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA31767.1"
                     /translation="MIRVLLFEDNNDLAESLAELIKDSDDIRLVAHYTNAQKAERHVA
                     YHHPDVVLMDIDMPVENGLQGLRAIRASGSGVMVIMFTVMEDNENVFQAICHGASGYL
                     LKQTAPEKILEGIREAMGGGAPMTPSIARKVLTLFAQPFKKSDDLQKLTAREHDVLSL
                     LVRGHSYKMAAAQLDIGVETLRFHIKNIYTKLHVNSKSEAVAKALQNRVV"
     gene            1606..3537
                     /locus_tag="PL106_01490"
     CDS             1606..3537
                     /locus_tag="PL106_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA31768.1"
                     /translation="MAGAGKRKRSLCVLVLVGVTIRAIAQAPPDIDSCKAVWTNTTFS
                     LDERAKALSYVLYADTSSDALEKAWLLLGKGKADPDDPVWKRAYAADAMVRARRTGQH
                     DDHEGAAKWFAIAAERYKAIGNKRFETEAQRWCGYALNNLGRFREAAPRLMAAERGYK
                     ELKDSTGITDVCCSLGYAFITQGSYDLAKAQFLRGLSYSHGRDDQWEFTMLYKIAECE
                     FDGGRYDSARVYLDRARYFMQHHEVEDYDNTVMSLEADFALREKRCDEALTVYRTILD
                     TMRKKDFMADRIASEMLFVADAQACARQYEQTYRTAHDALLIAQRIGMLELQERALRI
                     MANAKEALGEPEEALRLQKRMVAVRDSLAHNESLSVLSSSLLGMEVEQQLRADSLSAA
                     LAISEARATADRSRAQRIILISLTALILVIAALLVNRYRLKRRLQLEQLRTRLARDLH
                     DDIGGTLSSISILSNVVKKRAEASGDTDGAASLEKISERSQRLMRDMSDIVWSVDPGR
                     DSMADLVGRMREFGAAVLEPKGIAFHFNAPENVLARELPVEVKKNLYLIFKEAVNNAA
                     KHAGARSVFVDVSMNARSFHVQVEDDGAGMRADAPVTGHGGNGLRNMRARAQEIGALF
                     STGTSERGGVRVAVELVMS"
     gene            3558..>5119
                     /locus_tag="PL106_01495"
     CDS             3558..>5119
                     /locus_tag="PL106_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HNA31769.1"
                     /translation="MFLHSRAPVLLAIALMLMGCAEQAPDAPSHRSPAFDSTFHARTL
                     AALHPASMRSDTIFCDSILKVYDDTALARMHPGRVNDAFVYAIISHDGYPTASMERFY
                     RLARYADNDRMLAWHDFLIARWRMMNGDHVEAVRLYQNLLTRFERMQDGAGISSACRR
                     LGQIYRLLGDFEVALPYLHRALPSEPRAEFRCNMLYAMGECHAHAHRADSVRWCLDRI
                     AEMAGDSMLLQRGDDRVLLYTMRLGLDAATIEARRTGRGDAMALVRDMARLDSLLEHR
                     DPWLGFIEAHDAASHIELAEEVVRALTALNRTDLARTLVLEAEERARDCTDCTLEEVA
                     LYAAVADMHIALGDRSEALRYQTLRADALTRNEVGKARLAVEQARQREEFQQQQSGMA
                     QALEQERLQARAMDIEYRMQRISLVVMIGFVVLFAGVLFVRLRINRRMQLEEVRTRLS
                     RDLHDDIGSTLSSINILTSLARKKAEAGDVEGAAASLTGISERSQRLQRNMSDIVWSV
                     DPDRDSLEELLVR"
BASE COUNT         1003 a         1573 c         1614 g          929 t
ORIGIN      
        1 ccatgtacga gtcgccgatg gtcttgatct tctcaatgcc atgcttttcg cagatgcggt
       61 cgaaggcgct gaagcattcg ttgaggtcgc gcaccagatc cctcggggcc atgtgttcgc
      121 tgatggtggt gaagcccttg aagtcggtga agaggatggt gacctggtcg atgagcttgg
      181 cgtcggcttc gcccttggcc ttcaactctt cggccacttc ctccggcagg atgttcagca
      241 gcagggcatc gctgcgcttc ttctcggcgc gcagttcctc ggtgcgctgc atcacctccc
      301 gctccaggtc cttgttgcgg gtgtggatgt cgccgtaggt catgtactcg ccctcgcgca
      361 gccggtagtc ggggtcgctg ccatgtacgt gcgtcaccag ccaattgccg gcaacgcgct
      421 tcaggatggt ggtctgccgc aagaggtcac gcgtggcttc gggcccactg cccatggtcc
      481 agtacgtatc gccctccacc caggcggttt cgtgccaaat acgcacttcc ttccagatgt
      541 actcgatggt gaacgcatgc ggatactgtt ccacgccctt ctcattgaac gcccggtatt
      601 gcgctttgcc aacggcgcgc tcgtgcagcg cggtaccgaa gaaggtgatg tcatccgccg
      661 ccacagcgaa gcgcccatcg aggtcgcgac gcgcatagca ctgccagaac tggtcatgca
      721 cggcgagcac ttccgtctcg gtcgcggtcg gctcccggat ggtgccgtct ttctccatgc
      781 gcaggacccc ggcttgcata aggcaagttt ccggcgtggc gtgaagttag cgtcccccac
      841 gaactggggg gtgcgtcgtg accagcaccc atccgttctt tgtatccacc atgatccgcg
      901 tactgctctt cgaggacaac aacgacctgg ccgaaagcct ggccgaactg ataaaggatt
      961 cggatgacat ccgcctggtc gcgcactaca ccaacgcgca gaaggccgaa cgccatgtgg
     1021 cctaccatca tccggatgtg gtgctcatgg acatcgacat gccggtggag aatggcttgc
     1081 aaggcctgcg ggccatccgt gcttcaggta gcggggtgat ggtgatcatg ttcaccgtga
     1141 tggaggacaa cgagaatgtg ttccaagcca tctgccacgg cgccagcggc tacctgctca
     1201 agcagaccgc tccggagaag atcctggaag gcatccgcga ggccatgggc ggcggcgcac
     1261 ccatgacccc gagcatcgca cgcaaggtgc tcacgctgtt cgcgcagccg ttcaagaaga
     1321 gcgacgacct gcaaaaactc accgcgcgtg agcatgatgt gctatcgctg ctggtgcgtg
     1381 gccacagcta caagatggcc gccgctcaat tggacatcgg cgtggagacc ctgcgcttcc
     1441 acatcaagaa catctacacg aagctgcacg tgaacagcaa gagcgaagcg gtggcgaagg
     1501 cgttgcagaa cagagtggtt taggcgggcg gtctggatga ggtcgcccag cgctcaacgc
     1561 ctcacccacc acccgatcca acgtgtggaa ctacctttta ccccgatggc aggagcgggc
     1621 aagcggaagc gatcactgtg cgtgctggtg ttggtcggtg tgaccatccg cgccatcgct
     1681 caagcccccc cggatatcga ttcgtgcaag gccgtgtgga cgaacaccac cttttccttg
     1741 gacgaacgag cgaaagcact gagctacgtg ctgtatgccg acacttcctc ggatgcgctt
     1801 gaaaaggcct ggcttctgct cgggaagggc aaggccgatc cggatgatcc ggtgtggaag
     1861 cgcgcgtatg cggcggacgc gatggtgaga gcacggcgga cgggccagca cgacgatcac
     1921 gaaggcgccg cgaaatggtt cgcaatcgcc gcagaacgct acaaggccat tggtaacaag
     1981 cgcttcgaaa cggaggctca gcgctggtgc ggctatgcgc tcaacaatct cggtcgcttc
     2041 cgcgaagcag ccccccgcct gatggccgcc gaacgaggct acaaggaact gaaggacagt
     2101 accgggatca cggatgtatg ctgcagcctg ggctacgcct tcattacgca ggggagctac
     2161 gatctcgcga aagcccagtt cctgcgcggc ctttcctata gccacggcag ggacgatcaa
     2221 tgggagttca ccatgttgta caagatcgcg gaatgcgagt tcgatggcgg tcgctacgat
     2281 tcggcacggg tctatctgga ccgcgcccgg tacttcatgc agcaccatga agtggaggac
     2341 tacgacaaca cggtcatgag cctggaagcc gacttcgcgc tacgggaaaa gcgatgcgat
     2401 gaagcgctca ccgtttaccg caccatcctg gacacgatgc ggaagaagga cttcatggcg
     2461 gaccgtatcg ccagcgagat gctcttcgtt gccgatgcac aggcatgtgc acgacagtac
     2521 gaacagacct accgcacggc ccatgatgcg ctcctgatcg cgcagcggat cggtatgctc
     2581 gagcttcagg agcgcgccct gcgcatcatg gccaacgcca aggaagcgct cggcgaaccc
     2641 gaagaagccc tgcgcctaca gaaacgcatg gtggcggtgc gcgacagtct tgcccacaac
     2701 gagagcctga gcgtattgag cagttcattg ctcggcatgg aggtggagca gcagttgcgt
     2761 gcggacagct tgagcgcggc cctcgccatc agcgaagcgc gcgcaacggc tgaccgttcg
     2821 cgggcccaac ggatcatact catttcactc accgcgctca ttctggtgat cgccgcgttg
     2881 ctcgtgaacc gctaccgcct gaagcgccgc ctgcaattgg agcagctccg aacacggctg
     2941 gcgcgcgacc tgcacgatga catcggcggc acgctgagca gcatcagcat cctgagcaac
     3001 gtggtgaaga aacgcgccga ggcgagcgga gacaccgatg gcgccgcttc cttggagaag
     3061 atcagtgagc gcagccaacg cctcatgcgc gacatgagcg acatcgtatg gagcgtggat
     3121 cccggccggg acagtatggc agacctggtg ggccgcatgc gcgagttcgg cgctgccgtg
     3181 ctggaaccca aaggcatcgc cttccatttc aacgctccgg agaacgtgct ggcgcgtgag
     3241 ctacccgtgg aggtgaagaa gaacctctac ctcatcttca aggaagcggt gaacaatgcg
     3301 gcgaaacatg ccggcgcgcg cagcgtcttc gtggacgtct ccatgaacgc gcgatcgttc
     3361 catgtgcaag tggaggatga tggcgccggc atgcgcgcgg atgcgccggt gaccggtcac
     3421 ggcggcaacg gcctgcgcaa catgcgcgca cgtgcgcagg agatcggcgc gctcttcagc
     3481 accggcacga gcgaacgcgg tggagtgcgg gtggccgtgg aactcgtcat gagctgaacg
     3541 gatcccgccg aacgaacatg ttcctccatt cacgagcacc ggtccttctt gccatcgcac
     3601 tgatgctgat ggggtgcgcc gagcaagctc ctgatgctcc ttcacatcgt tcacccgcat
     3661 ttgacagcac cttccatgcg aggaccttgg ccgcgctgca cccggcctcc atgcgcagcg
     3721 acaccatctt ctgcgatagc atcctgaagg tgtatgacga taccgcgctc gcgcgcatgc
     3781 atccgggccg tgtgaacgac gccttcgtgt acgcgatcat ttcgcacgac ggctatccca
     3841 cggcctcgat ggagcgtttc tatcggctgg cgcgatacgc ggacaacgat cgcatgctcg
     3901 cctggcacga cttcctgatc gcccgatggc ggatgatgaa cggcgatcat gtggaggcgg
     3961 tacgcctcta tcagaacctg cttacgcgat tcgagcgcat gcaggatgga gccgggatat
     4021 cctccgcctg ccgtcggctg ggccagatct accgtctatt gggcgatttc gaggtcgcct
     4081 tgccctacct ccatcgcgcc cttcccagtg agccgcgggc ggaattccgt tgcaacatgc
     4141 tctacgcgat gggtgagtgc catgcgcatg cgcatcgcgc tgattcggtg cgctggtgcc
     4201 tggaccggat cgctgagatg gccggcgata gcatgctgct gcagcgcggc gatgaccgcg
     4261 tactgctgta taccatgcgc ttggggctcg atgcggcgac catcgaagcg cgtcgcacgg
     4321 gtcgaggaga tgccatggcc ctggtgcgtg acatggcgcg gttggacagc ttgctggagc
     4381 atcgtgaccc ctggctgggg ttcatcgaag cccatgatgc cgcatctcac atcgaattgg
     4441 ccgaagaggt ggtacgcgcc cttaccgcct tgaaccgaac ggatctcgcg cgaacgctgg
     4501 tactggaggc cgaagagcga gcccgggact gcacggactg tacgctggag gaggtggctc
     4561 tttatgccgc cgtggccgat atgcacatcg cgttgggcga ccggtccgag gcgctgcgct
     4621 accaaaccct gcgcgccgat gcgctcacgc gcaacgaggt gggcaaggcc cggcttgccg
     4681 tggagcaggc gcgccaacgc gaggagttcc aacaacaaca atccgggatg gcgcaagcgc
     4741 tcgagcaaga acgcttacag gcccgcgcca tggacatcga ataccgcatg cagcgcatca
     4801 gcctggtggt gatgatcggc ttcgtggtcc tgttcgccgg cgtgctcttc gtgcgcctgc
     4861 gcatcaaccg gcgcatgcaa ttggaggagg tgcgcacgcg cctcagccgc gacctgcacg
     4921 acgacatcgg cagtacgctc agcagcatca acatcctaac cagcctggcg cgcaagaaag
     4981 ccgaagcggg tgacgtggaa ggagcggcgg cttcactcac cggcatcagc gagcgcagcc
     5041 agcgcctgca gcgcaacatg agcgacatcg tgtggagcgt ggacccggac cgcgacagtc
     5101 tggaggaatt attggtgcg
//
LOCUS       DAOQOC010000054         5034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6899_length_5034_cov_3.658767, whole genome shotgun sequence.
ACCESSION   DAOQOC010000054 DAOQOC010000000
VERSION     DAOQOC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_5034_cov_3.658767"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1093)
                     /locus_tag="PL106_01500"
     CDS             complement(86..1093)
                     /locus_tag="PL106_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31770.1"
                     /translation="MSITRSTLSLAALACAAMLSAQTFDLDQFDQLFRPRLRLESRWL
                     PRTDVIREPGTYEDRSASAVLTFPIHSTFNVGAQLDLTAGSLQELIKNGIRARASQVM
                     GTLRYGAREVRFDPQLGGPRMLHTATAGALGVSLTKRYRILFWSLNVNVSEEDRTMDR
                     AVPRFGGLIGSMRVKGLRRQFFYGLAIAYSDKLPIPIPFLGGSAPMGPRWSFNYVLPL
                     QFSFTDRIGSRTRLDLGAGLDGSRTGIALLNERCNMNWSGVRGFLSVRHRLGAHLALR
                     LEGGYMFAHRVSFGTPGGDRADPPYALRPGPSITAGVNVLFGKSVLQRVMDEVLDRAL
                     P"
     assembly_gap    1276..1375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1613..2245)
                     /locus_tag="PL106_01505"
     CDS             complement(1613..2245)
                     /locus_tag="PL106_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA31771.1"
                     /translation="MDLFGDDLYFDEHGEIHVQQRNLPHWKQEGKLYFLTWRLADSLP
                     QEQLAQLDADRAAWVRRHGTALVRHLSPALREEWYNLFHRRVQRWLDAGYGSCVLKHP
                     EAMRIMVDSLHFFEGQRYRLGSFAIAGNHVHVLVAPLVGIDLSDVLHSWKSFTGNAIN
                     KAIGQRGRLWMDENFDRLVRDAEHLNRIEAYIARHEHQGAHVELRDLFNP"
     gene            complement(2343..3377)
                     /locus_tag="PL106_01510"
     CDS             complement(2343..3377)
                     /locus_tag="PL106_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNA31772.1"
                     /translation="MSTTNGTPTAPKKIDLSSDQEVRWCPGCGDYSILKQVQTLLEQS
                     GRKKEEIVFISGIGCSSRFPYYLDTYGLHGIHGRAPAIVSGLRSVRPELSVWMITGDG
                     DSLSIGGNHLIHLMRRNVDVNVLLFNNEIYGLTKGQYSPTSPQGAVTRSTPLGSTDHP
                     FNPLALVKGADGTFIARSMDRDPKHLREVLARADAHRGTSLVEIYQNCNVFNDGAFET
                     FTEKATKPLTTIFVEHGKPLVFANGTRGIKLNGMTPVIVDTYAGGASLDELWIHDERD
                     AFKASILVRLFEDPRLEGAMPRPFGVFFVNDRPTHEAKLTTQLAQARARKGPGDLDAL
                     LKGEHTWTIG"
     gene            complement(3374..>5034)
                     /locus_tag="PL106_01515"
     CDS             complement(3374..>5034)
                     /locus_tag="PL106_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNA31773.1"
                     /translation="YQLHFGSVEIWTPGDQCDVLVVMNAAALKANLGKLKRGGVIIAN
                     TDGFDKKNLRLAGYLDEADPLTDGSLADHTLHSVDVTKLTRTALEGTTLGMKEKDRCK
                     NMFVLGFINWMYNRDNALTEGFLKKKFAKRPELLDANMKALIAGYNYGDTSETFTTRF
                     EVKPAPMPKGTYRSITGNQGTALGLVAAAQRSGLDLFYGSYPITPASDILHELSKHKN
                     FGVITFQAEDEIAAISSAIGASYGGALGITASSGPGIALKTEALGLAFMLELPLVIVN
                     VQRGGPSTGLPTKTEQADLWQAVHGRNGEAPIPVIAASTPIDCFDMAFEAVQIALEHM
                     TPVILLTDGYIANGSEPWRFPPSKDLPAIKPPYIKEPNDGEKFLPYLRDERGVRPWAL
                     PGQPGLQHRIGGIEKQDKTGNISYDPANHEMMVRLRAEKVARIADRYKPIRLDSGPAD
                     GDLLIVGWGSTYGAIRTAALKLQQEGHSVAHVHLRHMFPFNKGLGPLLKKYRKILIPE
                     MNSGQLRQLVRAEFLCDAQGLNKIQGMPFTSDEIRAGVLNHLRP"
BASE COUNT          874 a         1488 c         1554 g         1018 t
ORIGIN      
        1 cctataccca cctgttcaca ggaagctatt caccgttcgc gctgacggga ggggtccgat
       61 tcggatacct gttctgagct gggcctcagg gcagggcccg gtcgagcacc tcgtccatca
      121 cgcgttgcag cacgctcttg ccgaatagca cgttcacccc cgcagtgatg gaagggcccg
      181 ggcgtagggc gtagggcggg tcggcacgat caccgcccgg agtcccaaag gatacccgat
      241 gcgcgaacat gtacccgccc tccagacgca gggccaggtg tgcacccaac ctgtggcgca
      301 cggagaggaa accgcgtact ccgctccagt tcatgttgca gcgctcgttc aggagtgcga
      361 tcccggtgcg cgaaccgtcg aggcccgcac caaggtccag ccgcgtacgt gatccgatcc
      421 tgtcggtgaa ggagaattgg agcggcagca catagttgaa ggaccagcga gggcccatcg
      481 gagccgagcc gccgaggaag gggattggga tcggcagctt gtcgctgtac gctatcgcca
      541 ggccgtagaa gaattgacga cgcaggcctt tcacccgcat ggagccgatg aggccaccga
      601 agcgtgggac tgcccggtcc atggtacggt cctcctcgct cacgttcacg ttcaagctcc
      661 agaagaggat gcggtagcgt tttgtgaggc tcacaccgag tgcacccgct gttgccgtat
      721 gtagcatgcg tggcccaccg agctgtgggt cgaaacgcac ctcgcgcgct ccgtagcgca
      781 gggtgcccat cacttgcgaa gcgcgcgcac gaatgccgtt cttgatcagc tcctgcaggc
      841 tgcccgccgt cagatccagc tgcgcaccca cgttgaaggt gctgtggatc gggaaggtga
      901 gcaccgccga ggccgatcgg tcctcgtagg tgccgggttc acggatcaca tccgtcctgg
      961 gcagccatcg gctctccaag cggagccgtg gtcggaagag ctggtcgaac tggtcgaggt
     1021 cgaaggtttg cgcggaaagc atggcggcgc aggcgagcgc ggccaatgag agcgtggatc
     1081 gtgtgatgct catggtccag tggcgaagtt cacccgtacc ggggtcacag gcttcaagct
     1141 tctgaagtgt ccttgggtca caggcttcca gcctttgcag tgtccttggg tcacaggctt
     1201 ccagcctttg cagtgtcctt gggtcacagg cttccagcct gtgaagagcg caccacagat
     1261 gctgggtaac aggctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccag
     1381 cctttgcagt gtacttgggt aacaggctcc cagcctgtga agagcgcacc acagatgctg
     1441 ggtaacaggc ttccagcctt tgcagtgtac ttgggtaaca ggctcccagc ctgtgaagag
     1501 cgcaccgcgg atgctgggta acaggcttcc agcctgtaca gtgtacttgg gtaacaggcc
     1561 tccagcctgt gaagagcgca tcaccggctc gaagcctgtg atccggtatc gttcatgggt
     1621 tgaacaggtc gcggagttcg acatgcgcgc cttgatgttc atgcctcgcg atgtacgcct
     1681 cgatgcggtt cagatgctca gcatcgcgta cgagccgatc aaagttctca tccatccaaa
     1741 ggcgtcctcg ttggccgatc gccttgttga tcgcgttccc ggtgaaagac ttccagctat
     1801 ggaggacatc cgacaggtcg atccccacca gcggagccac aagtacatgc acatggttcc
     1861 ctgcgatggc gaaactgccg agccgatagc gttgcccttc gaagaagtgg agcgagtcga
     1921 ccatgatgcg catggcctcg ggatgcttca ggacacacga gccgtacccg gcatccagcc
     1981 aacgctgcac ccggcggtgg aacaggttgt accattcctc cctcagcgcg ggcgacaggt
     2041 gcctcacgag cgcggtgcca tggcggcgca cccatgcggc gcggtcagcg tccaactgcg
     2101 ccagttgttc ttgcggaagg ctgtcggcca atcgccaagt caagaagtag agcttgcctt
     2161 cctgtttcca atgaggaagg ttgcgctgtt gaacgtgtat ctcgccgtgt tcgtcgaagt
     2221 agagatcgtc gccgaaaagg tccatggtct gaagatactc gcatgtgcat ttcgacacag
     2281 gctggaagcc tgtgaccagg cagcgttccc gggtcacagg cttccagcct gtgcagaaca
     2341 acctacccga tcgtccacgt atgctcgccc ttgagcaggg catccaggtc gccggggcct
     2401 ttgcgcgctc tcgcttgcgc caactgcgtt gtcaacttgg cctcgtgcgt gggtcggtca
     2461 ttcacgaaga acacaccgaa aggccttggc atggctccct ccagacgagg atcctcgaac
     2521 aatcgcacca gaatgctcgc cttgaaggca tcgcgctcat catggatcca caactcgtcc
     2581 aggctcgcac caccggcgta cgtgtccacg atcacgggcg tcatcccgtt cagcttgatg
     2641 ccacgtgtgc cgttcgcgaa caccagtggc ttgccgtgtt caacgaaaat ggtggtcagc
     2701 ggcttcgtgg ccttctcagt gaacgtctcg aacgcaccgt cgttgaacac gttgcagttc
     2761 tggtagatct ccacgagtga tgtgccgcgg tgcgcatcgg cccgggcaag gacctcgcgc
     2821 aagtgcttgg gatcgcggtc catgctgcgg gcgatgaagg tgccgtccgc ccccttcacc
     2881 agcgcgagcg gattgaacgg gtggtccgtg ctgcccaacg gtgtgctgcg cgtcactgct
     2941 ccttggggac tggtagggga gtactgtcct ttggtcagcc cgtagatctc gttgttgaag
     3001 agcagcacgt tgacatccac gttgcggcgc atgaggtgga tgaggtggtt gccaccgatg
     3061 ctgagcgagt ctccatcccc cgtgatcatc cacacgctca actcggggcg tacgctgcgc
     3121 aacccgctca cgatggccgg cgccctgcca tggatcccgt gcaacccgta tgtatcgagg
     3181 tagtacggga agcgggagct gcatccgatg ccgctgatga agacgatctc ctccttcttc
     3241 ctgccactct gctccagcag ggtctgcacc tgcttcagga tgctgtagtc gccgcaaccg
     3301 gggcaccagc gcacttcctg gtccgatgag aggtcgatct tcttcggcgc ggtgggtgtg
     3361 ccgttcgtgg tgctcatggt cgcaagtggt tcagaacgcc cgcacggatc tcatcgctgg
     3421 tgaacggcat gccctggatc ttgttcaagc cctgcgcgtc gcagaggaat tcggcgcgca
     3481 ccaattgacg gagctgaccg ctgttcatct cggggatcaa tattttcctg tacttcttca
     3541 acagcggccc cagccccttg ttgaagggga acatgtggcg gaggtgtacg tgcgccacgc
     3601 tgtgaccttc ctgctgcagc ttcaatgcgg cggtgcgtat ggcgccgtag gtggagcccc
     3661 agccgacgat caacaggtcg ccatccgcag gcccgctgtc caaccggatc ggtttgtagc
     3721 gatcagcgat ccgcgcgacc ttttctgccc gcaaccgaac catcatctcg tggttggccg
     3781 gatcgtagct gatgttgccg gtcttgtcct gtttctcgat accgccgatg cggtgctgca
     3841 ggccgggctg tccgggcagg gcccatgggc gaacaccgcg ttcatcacgc aggtagggga
     3901 ggaatttctc gccgtcgttc ggttccttga tgtacggggg cttgatcgcg ggcaggtcct
     3961 tcgagggggg gaaacgccag ggttccgatc cgttcgcgat gtaaccatcg gtgagcagga
     4021 tcacgggcgt catgtgttcc agtgcgatct gcacggcttc gaaggccatg tcgaaacagt
     4081 cgatcggtgt gctggcggcg atcacgggaa tgggggcctc gccgttgcgg ccatgcacgg
     4141 cttgccacag gtccgcttgc tcggtcttcg tgggaagtcc ggtgctgggt ccgccgcgtt
     4201 gcacgttcac gatcaccagc gggagttcca gcatgaaggc gagtcccaac gcttcggtct
     4261 tcagggcgat cccggggcca ctgctcgccg tgatgccgag cgcaccgcca tagctcgctc
     4321 caatggcgct gctgatggca gcgatctcat cctccgcctg gaaggtgatc accccgaagt
     4381 tcttgtgttt ggagagttcg tgcaggatgt cgctggcggg cgtgatcggg taactgccat
     4441 agaacaggtc gagtccggat ctctgcgcgg cggcgacaag accgagggcc gtgccctggt
     4501 tgccggtgat gctgcggtag gtgcctttcg gcatgggcgc gggcttcacc tcgaagcgcg
     4561 tggtgaaggt ctcactggta tcgccgtaat tgtacccggc gatcagtgcc ttcatgttcg
     4621 catcgagcaa ctcgggccgc ttggcgaact tcttcttcag gaacccctcc gtgagcgcat
     4681 tgtcccggtt gtacatccag ttgatgaaac cgagcacgaa catgttcttg caccggtcct
     4741 tctccttcat gccgagggtg gtgccctcca gcgcggtacg cgtgagcttg gtcacatcca
     4801 cgctgtggag cgtgtggtcg gccaggctgc catcggtgag cgggtcagct tcgtcgagat
     4861 acccggccag gcgcaggttc ttcttgtcga aaccgtccgt attggcgatg atgacgccgc
     4921 cccttttcag tttgccgagg ttggccttga gggccgcggc gttcatcacc acgagcacat
     4981 cgcactggtc accgggtgtc cagatctcca cactgccgaa gtggagctgg tagc
//
LOCUS       DAOQOC010000055         5034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6900_length_5034_cov_3.629042, whole genome shotgun sequence.
ACCESSION   DAOQOC010000055 DAOQOC010000000
VERSION     DAOQOC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6900_length_5034_cov_3.629042"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="PL106_01520"
     CDS             <1..1832
                     /locus_tag="PL106_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA31774.1"
                     /translation="SDGLQSDEFNRYAYCKATDGTLFFGGVKGFNHFHPRDLVDDTLA
                     GAIRITGIKLLNKVLDFRAGGSPLTMPTYLSEGMSIPHSVNMVTFEFATMEFSAPEEH
                     RYQYMLDGFDADWIMAGTDHSAGYTNLDPGNYTFRVRGDNRDGNWDRTGTSFRLIVWP
                     PWYRTWWAYSIALLVTVGGAWLYVRHLRRQKTLLEEVVLERTSALSAAKERAEQSERV
                     KQQFLANMSHEIRTPMNAIVGMSTVLRRNEHLPAQQAHLDAIASSSENLLVIVNDILD
                     LSKIEAGKLELEKVRMDPRAILDEVMEVMRFRAEEKGLRLETCVAEELPDAVMGAPTR
                     LHQVLMNLIGNGIKFTERGSVQVSMCVQERLSDAVMLRCEVKDTGIGIAPRRLARVFD
                     EFTQADEDHTRKFGGTGLGLTICKRLVEMQGGTISATSVPGQGSTFGFTIPYTTVPVG
                     APVDQADDAQPSPDTRTSELRDLRILLAEDNKLNVMVAEEELKDAIPGVRVDVAVNGK
                     MALDMHAVERYDLILMDVQMPVMDGYEATRVIRTFGEGDNKRPGMREKARVPIVAMTA
                     NVMQAEVQQCMEAGMDGFVPKPFKQAELVEAIRAAIALAGTGR"
     gene            1918..>5034
                     /locus_tag="PL106_01525"
     CDS             1918..>5034
                     /locus_tag="PL106_01525"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNA31775.1"
                     /translation="MKHPALLNTAVALSIGFASINGHAQSNQIGQPVNGSVGGVNGLY
                     LTVFGTTGPGCGVMNYRYTMADPSTPWGSSYGGPETYYALPATKEQQSFNVAFTYASA
                     NTAQPTYEVFVDGQYCASCVATVNSHSVTGIPGITDIEIQDYITITHQAADAWGAPNN
                     GVREFGLRYKMGGQAIADVRFKVVFIGAVFTNVLGYYNTPALPQYILRDPPGDGSYSS
                     ITLTNGACVGETRAVTTGNTQNGYFKARIGVSFTLGFVMTVPVEIYGEIGADLTATQT
                     ETENFENLTCLESSTTYSTSAIGPPGDLFLLTATRFAYGMAKIIERPTCGVINKTNYM
                     VTVPVENLGSYSATENDIRNVLMPDIQEMLQTLDPVADSVAYKAGLQQLSVWQQTLDM
                     NDSIKADAVAGAPPENFFGAGAVIDHNLTTTTTESYSINYEAALDAGLSFEFGVNIGG
                     SGITAGGSAHFSNGYGVGQNNSNSVTNTVSYHMEDDDEGDRFRVRVLQDKVFGTYVFE
                     LDTVISETSCGYEGGYQREQPSLSVGAPGNNFMVVNEAPIGDPVAFPLYICNNSTDTM
                     TYYLKFRNESNTEGGQLEVLGDPLNSTGNGKDVTIPAGECLGVSNLVLTEPTLPIGPD
                     YSIELYLYSLCEPEISSSITIAAHFGPGNFGSYCEPTSATGPAAGDYIDGVQIGTISN
                     TGTGGEDGGTYMDYSAQQSTPLSRNAPSVLTITSGNNAGDSYAAWADWDQDNTFEANE
                     SLGQGPNATPGALVNLFFTVPDNATLGSTKLRVRGAHVVGNEPAILNPCYNYSLGETE
                     DYSVVVNANTPQDCLGANNGPAMPGTACNDNNANTGNDTWNANCQCVGTLVDCEGTPG
                     GTVGEGSPCDDGNANTVNDAFTQNCLCIGQTLDCEGIPGGQALPGEPCDDGLATTGGD
                     VYNAICDCSGFVIDCAGVIGGTTLPGTACNDSNPLTGGDAFNPNCICVGAFETDCAGV
                     QGGPAQPGTTCDDNDATTGADSYNLSCVCAGLPYDCMSVPGGTALPGTACDDQNMATD
                     NDLWTVGCV"
BASE COUNT         1086 a         1510 c         1534 g          904 t
ORIGIN      
        1 cgagcgacgg actccaaagt gatgagttca accgctatgc ctattgcaag gccactgatg
       61 gaaccctctt cttcggcggc gtgaagggct tcaaccattt ccatccacgc gatctggtgg
      121 acgacacgtt ggccggtgcc atccgcatca cggggatcaa actgctcaac aaggtcctgg
      181 acttccgggc aggggggtcg ccgctaacca tgccgaccta tctgagcgag ggcatgagca
      241 tcccgcacag tgtgaacatg gtcaccttcg agttcgccac gatggaattc tccgctccgg
      301 aggagcaccg ctatcaatac atgctggatg gcttcgatgc cgactggatc atggcgggca
      361 cggatcacag cgcggggtac accaatctgg acccgggcaa ttataccttc cgggtaaggg
      421 gtgacaaccg cgacgggaac tgggatagga cgggcacctc cttccgcctc attgtatggc
      481 cgccctggta ccgcacgtgg tgggcgtact ccatcgccct gctcgtaacg gtaggaggtg
      541 catggttgta tgtccgtcat ctgcgcaggc agaagacact tctggaggag gtggtgctgg
      601 agcgtacgtc ggcactgagc gctgcgaagg agcgggccga gcagagtgag cgtgtgaagc
      661 agcagttcct ggccaacatg agtcatgaga tccgcacgcc gatgaacgcg attgtgggaa
      721 tgagcaccgt attgcgtcgc aatgagcacc tgcccgcgca acaggcacat ctcgatgcga
      781 tagcgagcag cagtgagaac ctgttggtga tcgtgaacga catactcgat ctgagcaaga
      841 tcgaggcggg caaactggag ttggagaagg tgcggatgga tccgcgcgcg atcctcgatg
      901 aagtgatgga ggtgatgcgc tttcgtgcgg aggagaaggg attgaggctc gagacctgtg
      961 tcgcggaaga actgcctgac gcggtgatgg gagcgcccac ccggctgcac caggtactga
     1021 tgaacctcat cgggaacggg atcaaattca ccgagcgcgg ttccgtgcaa gtttccatgt
     1081 gcgtgcagga acgcttgagc gatgccgtga tgctgcggtg cgaggtgaag gacaccggta
     1141 tcggcatcgc accgaggcga ctggcacgcg tgttcgacga gttcacgcaa gcggatgagg
     1201 accacacgcg caagttcggc ggcaccggcc tcggtctcac catttgcaag cgactggtcg
     1261 agatgcaggg cggcaccatc agcgcgacca gcgtgccggg gcaggggagc accttcggtt
     1321 tcaccatccc gtacaccacc gttcccgtgg gtgctcctgt ggatcaggcg gacgatgcgc
     1381 aaccttcgcc cgacacaagg acatcggaac tccgggacct gcgcatcctg ctggccgagg
     1441 acaacaagtt gaacgtgatg gtggccgagg aagaactcaa ggacgcgatc cccggggtac
     1501 gcgttgatgt ggccgtgaac gggaagatgg ccttggacat gcacgcggtc gagcgatatg
     1561 atctcatcct catggacgta cagatgccgg tcatggacgg ttacgaggcc acccgggtca
     1621 tccggacctt cggtgaaggc gataacaagc ggcccggtat gcgcgagaag gcgcgcgtcc
     1681 ccatcgtggc gatgacggcc aacgtgatgc aggccgaggt acagcaatgc atggaggcgg
     1741 gcatggatgg tttcgtgccg aagccgttca agcaagcgga gctggtggag gcgatccgtg
     1801 cggctattgc gctggccggt acgggacgct gaacgcaaga acaggtattt ctaccgggtg
     1861 tggggcgacc gacgctggga acctttggtc cctccctcgg accaacctct cttcatcatg
     1921 aagcatcctg ctctcctcaa cacggccgtc gcgctctcca tcggcttcgc cagcatcaac
     1981 ggtcatgcgc aatccaatca gatcggccaa cccgtcaatg gcagcgttgg cggggtcaac
     2041 ggcctgtacc tcaccgtctt cgggaccacc ggccccggat gcggtgtcat gaactaccgc
     2101 tacaccatgg ccgacccgtc aacgccatgg ggcagctcct acggcgggcc ggagacctat
     2161 tacgcattgc ctgctaccaa ggaacagcaa tcgttcaatg tcgccttcac gtacgcttcg
     2221 gccaacacgg cccagcccac ctacgaggtt ttcgtggatg gccagtattg cgccagttgc
     2281 gtcgccacgg tcaattcaca ctccgtcacc ggcataccgg ggatcacgga catcgagatc
     2341 caggactaca tcaccatcac gcaccaggca gctgatgcat ggggcgcacc gaacaacggt
     2401 gtacgcgaat tcggactgcg ttacaaaatg ggcggacagg cgatcgcgga tgtacgtttc
     2461 aaggtggtct tcatcggcgc ggtgttcacc aacgtgctcg gctattacaa cacaccggca
     2521 cttccacagt acatcctgcg cgatccgccc ggcgatggca gctacagcag catcaccctc
     2581 accaacggtg cttgcgtggg cgagacccgt gccgtgacca ccggcaatac ccagaacggc
     2641 tatttcaagg cacggatcgg ggtttcgttc acccttggtt tcgtgatgac cgtgccggtg
     2701 gagatctacg gcgaaatagg cgcggacctt accgcgacgc agaccgagac cgaaaatttc
     2761 gagaacctca cttgcttgga atcctccacg acttacagta caagcgctat cggtccaccg
     2821 ggcgatctct tcttgctcac agcaacacga ttcgcgtacg gcatggcgaa gatcatcgag
     2881 cggcccacct gcggtgtcat caacaagacc aactacatgg tcacggtgcc cgtggagaac
     2941 ctgggcagct acagcgctac ggagaatgat atcaggaacg tgctgatgcc ggacattcaa
     3001 gagatgctcc agaccttgga tccggtggcc gatagcgtgg cctacaaagc cgggttgcag
     3061 caattgagcg tgtggcagca aacgctcgac atgaacgact ccatcaaggc cgatgcggtc
     3121 gcgggagcac ctcccgagaa cttcttcggc gcgggtgctg tgatcgacca caacctcacc
     3181 accaccacca ccgagtccta ctccatcaac tacgaagcgg cgctcgacgc aggactttcc
     3241 ttcgagttcg gtgtcaatat cggtgggtcc ggtatcaccg ccgggggctc ggcgcatttc
     3301 agcaacggtt acggtgtagg gcagaacaac tcgaacagcg tgaccaacac ggtctcctac
     3361 cacatggagg atgatgatga gggagaccgc ttccgtgtac gggtcctgca ggacaaggtg
     3421 ttcggcacct acgtgttcga actggacact gtgatctcgg agaccagctg tggctacgag
     3481 ggcggatacc agcgcgagca gccgtcgctc agcgtgggcg cgccaggcaa caacttcatg
     3541 gtggtgaacg aagcgccgat cggcgatccc gtagccttcc cgctgtacat ctgcaacaac
     3601 agcacggaca ccatgaccta ctacctgaag ttccgcaacg aatccaacac cgaaggcggg
     3661 cagttggagg tgctcggtga tccgctcaat tccacaggga atggtaaaga tgtcaccatc
     3721 cccgccgggg aatgcctcgg ggtctcgaac ctggtgctca ccgagccgac ccttcccatc
     3781 ggtcccgact acagcatcga actgtacctc tactcgctct gcgaaccgga gatcagttcc
     3841 agcatcacca tcgcggcgca cttcggcccg ggcaacttcg gcagctattg cgaacccacc
     3901 agcgccaccg gtcctgcggc cggtgattac atcgatggtg tgcagatcgg taccatcagc
     3961 aatacaggta caggcggtga agatggtggt acctatatgg actatagcgc acaacagagc
     4021 acgccgctct cccgcaacgc gccaagcgta ctcaccatca ccagcggcaa taatgcgggc
     4081 gacagctacg ccgcatgggc cgattgggac caggacaaca ccttcgaggc gaatgaatcg
     4141 ctgggccaag gacccaacgc cacccccgga gcactcgtca acctgttctt caccgttcca
     4201 gataacgcga cactgggctc cacgaaactg cgcgtacgcg gtgcgcacgt ggtgggcaac
     4261 gaacccgcca tcctcaaccc ttgttacaat tattccctcg gggaaacaga ggattattcc
     4321 gtggtggtca atgcgaacac accgcaggat tgcctgggtg cgaacaatgg ccccgccatg
     4381 ccgggcacgg cctgcaacga caacaatgcg aacaccggaa atgatacctg gaacgcgaac
     4441 tgccagtgcg tgggcaccct ggtggattgt gaaggcacac cgggcggcac cgtgggcgag
     4501 gggtcaccgt gcgatgatgg caatgccaac accgtgaacg atgcctttac acagaactgc
     4561 ctatgtatcg gccaaacgct cgattgcgaa gggatccctg gtggccaagc acttccaggc
     4621 gagccgtgcg atgatggcct cgccacgacc ggtggtgatg tgtacaatgc catctgcgac
     4681 tgttccggct ttgtgatcga ttgcgccggt gtcatcgggg gcaccacgct tcccggtacc
     4741 gcatgcaatg acagcaatcc cctgacaggt ggggatgcgt tcaaccccaa ctgcatctgc
     4801 gtcggtgcgt tcgagaccga ctgcgcgggg gtgcagggtg gaccggccca gccgggtacg
     4861 acctgcgacg ataacgacgc caccaccggt gccgacagtt acaacctgag ttgcgtttgc
     4921 gcgggcttgc cgtacgactg catgagcgtg ccgggaggga ccgcgttgcc cggcacggct
     4981 tgcgacgatc agaacatggc caccgataac gacctgtgga cggtcggttg cgtg
//
LOCUS       DAOQOC010000056         5015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6951_length_5015_cov_3.421573, whole genome shotgun sequence.
ACCESSION   DAOQOC010000056 DAOQOC010000000
VERSION     DAOQOC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_5015_cov_3.421573"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PL106_01530"
     CDS             complement(<1..253)
                     /locus_tag="PL106_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31776.1"
                     /translation="MMKRPFLTLALAVLAALSIGSGSARPQAPERGPARPDLLPAFLD
                     RPTPWADSVMASLDLNDRIAQLMMVAAYSNKGREHVRGIE"
     gene            532..2319
                     /locus_tag="PL106_01535"
     CDS             532..2319
                     /locus_tag="PL106_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31777.1"
                     /translation="MKRALLFPALVAAITTSAQIDTETGGLNNTCDPVSYDAIALDIP
                     DTAGLHLNTDMDFFRVEVPVGGVLQATVSSTSPGASFVVEIRDGSCASATSATGLVGQ
                     DNRAVAVVCPGAWFVGVSRATGTAPIAYVLTATITNSGECGTECDNTMSTATLLTALD
                     TVFETRLWGQNALIASGTAFPIPEDQDFYSLQTGACGGTLSIQVSDIPWNITLRVSCF
                     EGTPDAPLHVWSFQRSGCISGDLNGAWTLEPNKTWWLRFHDESTEQENCNSSYDLSPD
                     CFQVTLDFTSGCDGCDGGEANNLPAIATPLMLDTAFTTRLWGRNEQFATDSVLGRAED
                     QDIYALNTGAEGGTLNIAVTEIPEGITLRAGLYAGPPFTLEHMGTFQRQACFGGNLTG
                     AWSVEAGTSYFLRLYDESAEDTICGEAYNMSDSCFTVSLSFAPGICGATLILTANEGP
                     GPGEATAAVSNGTPPFTWAWSNGDTTQTAGGLDPGTYTVTATDSAGCTGSTTVMVIGT
                     SVSGREGNMIGIWPNPVEGPVTIRTANTARVQRIMLSDASGRIVASRLLTCANGVATL
                     DLGGLESGTYLVRVYFDDGGEEIGRVLKR"
     gene            complement(2351..4162)
                     /locus_tag="PL106_01540"
     CDS             complement(2351..4162)
                     /locus_tag="PL106_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNA31778.1"
                     /translation="MKQHIAVLALCMPLVGYPQSVENMVCTPPIAAQVMKGNYDPSAF
                     AASIPISDHATILCDLRTAISPDSLRAHLEGLTSFHTRHAFSDTLSPDTGIGAARRWA
                     FAKFQAFSSANEGRLIPAYLSFDYTGDPCGVDGYGWRDVLAVLPGADTSTSSIILMEA
                     HMDSRCADPCDNTCLAPGAEDNGSGSALVIELARVMSRWTFDHTIVFMLTTGEEQGLV
                     GAEAMAQWCVDEGVAIKGVQNNDVVGGIWCGATASDPGCEVENEADSLRVRLFSNGSP
                     PRPHRGFARSIKLWYEEKLRNEVPVPMTISVMGLEDRVNRGGDHIPFRQRFFRNVRFT
                     ASHEHGDGDPEDTTYHDRQHTDTDIIGVDTDGDLAIDSFFVDFNYLARNAVINGMSAA
                     LLALGPEPPAFELLDEPDGLRVAITPESGVSAYRVGVRLTNANLDFDQLYRTTDTSFV
                     VPGQVAGTQRWVSVASIDAEGITSPFSGELVRTSDIDTPPGTVDDLAYGLSCGSIAVP
                     EAARVEKLPPVITFKPNPFAMDTELSVQFPATAAWGRAQLLVRDALGREVAIVALRTG
                     PGRNTFVYRHTAGAGLFHFSLVAEDRVLASGRAMVVR"
     gene            complement(4269..>5015)
                     /locus_tag="PL106_01545"
     CDS             complement(4269..>5015)
                     /locus_tag="PL106_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31779.1"
                     /translation="VQFDANGSQNGLPVWSLGDGNTAEGMMLTHVYDSTGSYVIQLMI
                     TGAGGCTDTLDVPLMLVVHPSPTAAFTFHAEESLPELIHFTNTSIGATVLEWNFGDAG
                     ISGEEHPQHRYDLRSDGCEWPVRLSVMNAEGCADTTYRTVHAEISVVHFIANAFTPNG
                     AGPVINERFRPDFVVDMDVVARCDYRFTIFDRWGHKLFETTDPYAEWDGLFPDGSAVP
                     QGAYVWAVHMVHPCIPPSIRDREGHVNLVR"
BASE COUNT          975 a         1564 c         1609 g          867 t
ORIGIN      
        1 cctcgatgcc acgcacgtgc tcccgtccct tgttgctgta cgccgctacc atcatcaact
       61 gggcgatgcg gtcgttgagg tcgagcgagg ccatcacact gtcggcccaa ggtgtgggcc
      121 ggtcgaggaa ggcgggcaac aggtccgggc gggcagggcc gcgctcgggg gcctgtgggc
      181 gcgccgaacc gctgccgatg gacaaggcgg ccagcaccgc cagggcgagg gtgaggaagg
      241 ggcgcttcat catgggtgtt cggccaggaa cgtgccgcac atcaatggaa caacgggcga
      301 aactacccgg gcggggtgcc gtgaccggtc agggatccgc gcagttgtca acagaacgac
      361 cgtggaagcc cctgacccga ccgggttcgg caaaacgtcc agaggtcatt gtactatctg
      421 ttttattgta ttttatcgca aggacacatc acccgaacca cttatccacg atcgtgcaaa
      481 acagccgcac gccgcttccc tggccgtgta ctattgtcgc cgcgtacgac catgaaaaga
      541 gcactgctgt ttcccgccct tgtagcggcc atcaccacct ccgcccagat cgacaccgag
      601 accggagggc tcaacaacac ctgcgacccg gtctcctatg acgccatcgc actggacatc
      661 ccggataccg cagggctgca tctgaacacg gacatggact ttttccgcgt ggaagtgccc
      721 gtgggcgggg tgctgcaggc cacggtcagt tccacctccc cgggcgcatc gttcgtggtg
      781 gagatccggg atggatcctg cgcgtcggcg accagtgcta ccggcctcgt tgggcaggac
      841 aaccgggcgg tggcggtggt gtgccccggt gcctggttcg taggcgtatc acgcgccacg
      901 ggcacagcac ccatagcgta cgtgctcacg gccacgatca ccaacagcgg cgagtgtggg
      961 accgaatgcg acaacaccat gtccacggcc acactgctca ctgcgctgga taccgttttc
     1021 gaaacccgct tgtggggcca gaacgcgttg atcgcgtcgg gcaccgcctt ccccatcccc
     1081 gaggatcagg atttttattc gctccagacc ggtgcctgcg gtggcacact cagcatccag
     1141 gtcagtgata ttccgtggaa catcacgctc cgtgtgagct gctttgaggg cacgcccgat
     1201 gctccgttgc acgtgtggtc attccagcgg agcgggtgca tcagtggcga cctgaacggc
     1261 gcatggacat tggagcccaa caagacctgg tggctgcgat tccatgatga gagcaccgaa
     1321 caggagaact gcaacagttc atacgacctg tcacccgact gcttccaggt aacgctggac
     1381 ttcaccagtg gatgcgatgg atgcgatggt ggcgaggcga acaacctgcc ggccatcgcc
     1441 acacccctga tgctcgacac ggccttcacc acccggctct gggggcgcaa cgagcagttc
     1501 gcgacggaca gcgtactggg ccgagcagag gatcaggaca tctatgcgct gaacacagga
     1561 gccgagggtg gcacattgaa catcgcggtc acggagatcc ccgaagggat caccttgcgc
     1621 gccgggttgt acgcggggcc accattcacc ttggagcaca tgggtacatt ccagcggcag
     1681 gcctgcttcg gcgggaacct cacgggcgcg tggtcggttg aagcgggaac ctcgtacttc
     1741 ctgcgtctgt acgacgagtc agcggaagac acgatctgcg gcgaagccta caacatgagc
     1801 gatagctgtt tcacggtcag tctcagcttc gcaccgggga tctgtggggc cacactgatc
     1861 ctgacggcca acgaaggacc aggacccggc gaggcaacgg ccgcagtgag caacggtacc
     1921 ccgcccttca cgtgggcctg gagcaatggc gacacgaccc agaccgccgg agggctcgat
     1981 cccggcacat acaccgtgac cgccacggac tcggccggat gcaccggcag taccacggtc
     2041 atggtcatcg gcacgtccgt cagtggaagg gagggcaaca tgatcggcat ctggcccaac
     2101 cctgtggaag gtccggtaac gatccggacg gccaacaccg cacgggtgca acggatcatg
     2161 ctcagcgatg cgagcggccg catcgtggca tcgcggctgc tcacctgtgc gaacggggtc
     2221 gccacgctcg acctcggcgg actggagagc ggcacctact tggtccgggt atatttcgac
     2281 gatggcggcg aggagatcgg gcgcgtgctg aagcgatgaa cgacggttca ccgcgggtcg
     2341 ttatccccga tcagcgcacc accatggccc ggcccgaggc caacacccgg tcttcggcta
     2401 cgagcgagaa gtggaacagc ccggcaccgg cggtatgccg atagacgaac gtgttcctgc
     2461 ccgggcccgt acgcagggcc acgatggcca cctcccgccc caacgcatcc cggaccagga
     2521 gttgagcccg gccccacgcg gcggtcgcag gaaattgaac cgataattca gtatccatgg
     2581 cgaacgggtt cggcttgaag gtgatcacgg gcggaagctt ctccacccgg gcggcctcag
     2641 gcacggcaat ggacccgcag gacaggccgt aggccagatc gtccaccgtg cccggaggtg
     2701 tatcgatatc actcgtgcgg accagctcac cggagaacgg gctggtgatg ccttccgcat
     2761 cgatggaggc cacgctcacc cagcgctgcg taccggcgac ctgtccggga acaacgaatg
     2821 aggtatccgt tgtgcggtac aattggtcga agtccaggtt ggcattggtg aggcgaacac
     2881 ccacccggta ggccgagact ccggactccg gcgtgatggc cacccgaagt ccgtcgggct
     2941 catcgagcag ttcgaaggcc ggtggttcgg ggcccagggc caggagcgcg gccgacatac
     3001 cattgatgac ggcgttgcgc gccaggtagt tgaagtcaac gaagaaactg tcgatggcga
     3061 ggtcgccgtc ggtatccacg ccgatgatgt cggtgtcggt gtgctgccgg tcgtggtagg
     3121 tagtgtcctc cggatcgccg tcgccgtgct catgcgaggc cgtgaagcgc acgttgcgaa
     3181 agaagcgctg gcggaacggg atgtggtccc ctcccctgtt cacccggtct tccagtccca
     3241 tcacgctgat ggtcatgggt acgggcactt cgttacgcag cttctcctcg taccacaact
     3301 tgatggagcg ggcgaaacca cgatgcggcc ggggcggtga accgttggag aagaggcgca
     3361 cccgcaagct gtccgcttca ttctctacct cacatccagg atcgctcgcc gtggcaccgc
     3421 accagatccc cccgacgaca tcgttgttct gcacgccctt gatcgccaca ccttcgtcca
     3481 cgcaccactg ggccatggcc tcggcaccca ccagaccctg ctcttcgccg gtggtgagca
     3541 tgaagacgat ggtatgatcg aaggtccagc ggctcatcac ccgggccagc tcgatcacca
     3601 gggccgagcc gctgccgttg tcttccgccc cgggtgccag gcacgtgttg tcgcagggat
     3661 cagcgcagcg gctgtccatg tgggcctcca tgaggatgat gctcgaagtc gaagtgtcgg
     3721 cgccgggcaa tacggccagc acgtcccgcc aaccgtaccc gtccacgccg cagggatcgc
     3781 cggtgtagtc gaagctgagg taggccggga tgagacgacc ttcgttcgca ctgctgaacg
     3841 cttggaactt cgcgaatgcc caacgccgtg ccgcaccgat ccccgtgtcg ggcgagagcg
     3901 tgtccgagaa cgcatggcgt gtgtggaagg aggtgaggcc ttccagatgg gcccgtagcg
     3961 agtcgggcga gatggccgtc cgcagatcgc acaggatggt ggcgtggtcg ctgatgggta
     4021 tgctcgcggc gaatgcggaa ggatcgtagt tccccttcat cacctgagcg gcgatgggcg
     4081 gggtgcagac catgttctcc acggactgcg ggtagccgac cagaggcatg cagagggcga
     4141 ggacggcgat gtgctgtttc atggagcagg ggatggcgcg taaagctacc agtgagagga
     4201 tcggaaggcc cggccagcgt aaagggggta atggccgaat gtgccccctt gggatccgag
     4261 gtcgcggctc agcgcacaag gttcacatgt ccctcgcgat cgcgaatgga aggtggtatg
     4321 cacggatgca ccatgtggac ggcccagacg tacgcacctt gcggaaccgc actgccatcg
     4381 gggaacaggc catcccattc ggcgtacggg tcggtggtct cgaacaactt gtggccccac
     4441 cggtcaaaaa tggtgaagcg gtagtcgcac cgggccacca cgtccatgtc caccacgaag
     4501 tccggacgga agcgttcgtt gatcaccggt cccgcaccgt tcggtgtgaa tgcgttggcg
     4561 atgaagtgga ccacgctgat ctcggcatgc acggtcctgt acgtggtgtc cgcgcaacct
     4621 tccgcattca tgacggacaa ccgcaccggc cattcgcaac catcggaccg caggtcatac
     4681 cggtgctgcg ggtgttcctc gccgctaatt ccagcgtccc cgaagttcca ttccagcacg
     4741 gtggcaccga tgctggtgtt ggtgaaatgg atgagctcgg gcaaactctc ctccgcatgg
     4801 aaggtgaagg ccgctgttgg cgaaggatgc accacgagca tgagcggcac atcaagcgta
     4861 tcggtgcatc ccccggcacc ggtgatcatc agttggatca catagctacc tgttgaatcg
     4921 tatacatgag tgagcatcat cccttccgcc gtattcccat cacccaagga ccacacgggc
     4981 agcccattct gggagccgtt cgcatcgaac tgaac
//
LOCUS       DAOQOC010000057         5009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6965_length_5009_cov_3.728906, whole genome shotgun sequence.
ACCESSION   DAOQOC010000057 DAOQOC010000000
VERSION     DAOQOC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6965_length_5009_cov_3.728906"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PL106_01550"
     CDS             complement(<1..224)
                     /locus_tag="PL106_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31780.1"
                     /translation="MQYAPEDSAPVPIRCLVVVIHSRKRNLPSVGCAGPISLWMVQKC
                     RLFGRVKDRPRSPKFAPMGTVLHQLEAARKT"
     gene            276..1169
                     /locus_tag="PL106_01555"
     CDS             276..1169
                     /locus_tag="PL106_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31781.1"
                     /translation="MRTTTLIWLCAWNLSAMAQSWFPNGATWTYDYANFIITGYVAHE
                     VSGDTLIDGRLCKVIERTRVSSSGSNVDTLERTPFCVLDSAGVVWIHVPSEGLDTLYV
                     MDAVPGDQWMLAAVPEDCDSTAYLEVVDTGHVVLDGLPLRWLAVDLHPFPMHSPWVYQ
                     DTIVERLGSLRYYLAPQDPCLGGFDAAEGGPLRCYADAQVSFMSSSVSTCELNLGVDD
                     HSAGSHFQLFPDPGGDGFEVRSSQPIQAVAVRDAAGRMFLTKPGGSLVHVPAVQWPAG
                     CYLVAAEFGNGTRAVRKWIKP"
     gene            1166..1651
                     /locus_tag="PL106_01560"
     CDS             1166..1651
                     /locus_tag="PL106_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31782.1"
                     /translation="MRKLALQAAHAAGHDLMHLTVAEHATFDSIARRGCDRPCTWAQN
                     LLCYGYQQCIPPCTGGNATQRALHPMDPAPDVPIDPLTVFPNPACSYTTLAYTITEAL
                     DGAWLIVRQVDGREVKRMVVNQPQGQALLDTRDFVPGTYSVELVNAGARLATERLVLK
                     P"
     gene            1966..3120
                     /locus_tag="PL106_01565"
     CDS             1966..3120
                     /locus_tag="PL106_01565"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31783.1"
                     /translation="MRLDGTAIYDFNPGPANTPYPGLVGYDWGDINVQSDGKILCTGA
                     SQLDYAFGFDSPGWYSVFRLCDNARLDSTYDMRTTDGTIWSLEPTGEDQFFASGVFTT
                     YEGVPVGRIIRIWADGELDTTYQSPILKGYAKCFIQQPDGRMIVGGQFVLPNDPDTMH
                     LIRLLPDGALDSTFHNDTEYKHLPQFSFGDFAFGVNDIEQLADGNIIVGGNFTHMDGQ
                     LRRGVALVDSLGNLLNTAFTGAGCGLAAQINSTHTYSGLASIDPAPDGSIYLVGEFNG
                     FDDGVVSDTAQRMICRLHGLNVGVSEHQAKANDGLRVYPNPGSGQITVEWPGHLIKTM
                     RFVDAQGRCAYSITCASCVRADVNVPSSGLYVLIVEDEQGDRISTKLTKP"
     gene            3117..4001
                     /locus_tag="PL106_01570"
     CDS             3117..4001
                     /locus_tag="PL106_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31784.1"
                     /translation="MISTRWWLVLPILVSCTKAGAQTWCPTGAEWWYEHQNFGQAGYV
                     HAAYVGDTLVDGEPAQRLEAYATGYDFWIDMPYYMQYPSVITRQVGGQVSYRHNEAWY
                     TLFDLDAPPGAQWPLAGLGLFDDRTMAVLDTGHFVVDGIMLRWSEVEFIPPFGSWATD
                     TIVERAGYKNVFIEPWRSLMLEGIVQGLRCYADDGIAYSTGLTATCDLILSTEEFSAH
                     GSFHVHPNPGSDYLWVKCGSPVRTAEARDLLGRSVWSGRGPQAASQVDTSSWPPGCYL
                     VAAEFGNGTRAVRKWIKQ"
     gene            4234..>5009
                     /locus_tag="PL106_01575"
     CDS             4234..>5009
                     /locus_tag="PL106_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNA31785.1"
                     /translation="MSTKTAEKLKYKVKDMALAEWGRKEIELAEAEMPGLMALREKYG
                     KEKPLKGARIAGCLHMTIQTAVLIETLKELGAEVSWSSCNIFSTQDHAAAAIAKAGIP
                     VYAWKGMNGEEFDWCIEQTLFAFEGGKPLNMILDDGGDLTNMVFDKYPELVKDIKGLS
                     EETTTGVHRLHEREKNGTLVMPAININDSVTKSKFDNKYGCKESAVDAIRRATDVMMA
                     GKVAIVCGYGDVGKGTAASLRGAGARVIVTEIDPICALQAS"
BASE COUNT         1010 a         1440 c         1569 g          990 t
ORIGIN      
        1 gttttcctgg ccgcttcgag ctggtggagc acggtgccca ttggggcgaa cttaggcgat
       61 ctggggcggt ccttgacccg gccgaaaagc ctacatttct ggaccatcca cagggatatg
      121 ggtccggcac accccacgct cggcaggttg cgcttgcgcg aatggatcac gaccacaagg
      181 cagcggatcg gcaccggtgc gctgtcttcc ggtgcgtact gcatcgaatt ccgatcggcg
      241 gcaagggttg tgcaacgcgc caaatggatc aagcaatgag aacgacgacc ctcatttggc
      301 tatgcgcctg gaacctctcc gcaatggcgc aatcctggtt ccccaatggt gccacgtgga
      361 cgtacgacta cgccaacttc atcatcacgg gttacgtcgc ccacgaggtg agcggtgata
      421 cgctcatcga cggtcggctg tgcaaggtga tcgaacggac gagggtttcc agcagcgggt
      481 cgaatgtgga tacccttgaa cggaccccct tttgtgtcct cgactcggcg ggggtcgttt
      541 ggatccatgt gccctcagaa ggtctggaca cgctatacgt tatggacgcc gtgcccggtg
      601 atcaatggat gctcgcggcg gtgccggaag actgcgacag cacggcgtac ctggaggtgg
      661 tggacaccgg ccatgtggtg ctcgatggcc ttcctctgcg ctggctggcc gtcgatctgc
      721 atccgtttcc catgcattcg ccgtgggtct accaggacac gatcgtggaa cgcttgggat
      781 ccctgcgcta ctatctggct ccgcaagatc cttgtcttgg gggttttgat gctgctgaag
      841 gtggcccgct gcggtgctat gcggatgcgc aggtctcgtt catgtcaagt agcgtgtcca
      901 catgcgaatt gaacctgggc gttgatgatc atagcgctgg cagccacttc caactttttc
      961 ccgatcctgg tggcgacgga ttcgaggtgc ggtcatcgca gccgatccaa gccgtagcgg
     1021 tgcgggatgc cgcaggcagg atgttcttga caaaacctgg tggaagcctg gttcatgtgc
     1081 ctgccgtgca atggcctgcg ggctgctact tggtggcggc cgaattcggg aacggcaccc
     1141 gcgcggtgcg caaatggatc aagccatgag gaagctcgca ctacaggccg cgcacgccgc
     1201 cgggcacgat ctgatgcacc tgaccgttgc ggagcatgcg accttcgata gcatcgcccg
     1261 acgcggctgc gatagaccat gcacgtgggc gcagaatctg ctgtgttacg gctatcagca
     1321 gtgcatacct ccttgcaccg gtggaaatgc cacccaacgc gcgctgcatc cgatggaccc
     1381 ggcacctgat gtcccgatcg atccgcttac ggtctttccg aacccggcct gcagttacac
     1441 cacgctcgcg tacacgatca ccgaggccct tgacggggct tggctgatcg tgcgccaggt
     1501 ggatggtcgc gaggtgaaac gcatggtcgt gaaccaaccc caaggacaag cgctgttgga
     1561 cacccgcgac ttcgttccgg gcacctacag cgtggaactg gtcaatgcag gtgcccgctt
     1621 ggccaccgaa cgccttgtac tgaagccatg aagcatttat tcttcgccgg gctggccgca
     1681 tgttcggcca gcccaggcgg agcgcaggtg cctttgacct tggatacctc gttccgctgt
     1741 ggcttggtgt acgcaagcct cactgatgca caccccttgg ctgatggccg actctggatc
     1801 gttggaagaa tctcctttga cggaacgaat cctgtgccct cctccagaag gctgttgccg
     1861 aatggctcct tggacccaga aatcgggttg ggaagtctgt caggtggaac catagttgag
     1921 actccggagt atttctacag tccggccggt ggtggtcccc gccgcatgcg attggatgga
     1981 acggccatat atgatttcaa cccaggccct gccaacaccc cttaccctgg ccttgttggt
     2041 tacgattggg gagacattaa cgtacaaagc gacggtaaga tcctctgcac gggggcctct
     2101 caattggact atgcgttcgg ctttgactcg cctggctggt acagcgtgtt ccgcttgtgc
     2161 gacaatgccc gcttggacag cacgtacgac atgcgcacca ccgacggaac gatatggtcg
     2221 cttgaaccca cgggcgaaga ccagttcttc gccagtggtg tcttcaccac gtacgagggg
     2281 gtgcccgtgg gccgcatcat acgcatctgg gccgatgggg aactggacac cacttaccaa
     2341 agcccgatcc tgaaaggcta tgccaagtgt ttcatccagc aacccgatgg acgcatgatc
     2401 gtgggtgggc agttcgtgct tcccaacgac ccggacacca tgcacctgat ccgcctgcta
     2461 cctgatggtg cgctggacag caccttccac aacgacacgg aatacaagca tctaccgcag
     2521 ttctcttttg gcgatttcgc cttcggcgtg aacgacatcg agcagttagc ggacggcaac
     2581 atcatcgtgg gtggcaactt cacgcacatg gacggccagc tacgccgggg tgtcgccttg
     2641 gtggacagtt tgggcaacct gttgaacaca gcctttaccg gcgcaggttg tggcttggcc
     2701 gcgcagatca attcaacgca tacgtacagc ggcctggcct caatcgaccc agcaccagat
     2761 gggagcatct acctcgttgg ggaattcaac ggcttcgatg atggcgtggt aagcgatacg
     2821 gcacagcgga tgatctgcag gttgcatggg ttgaacgtgg gggtgtcaga gcaccaagcc
     2881 aaagccaacg atgggctaag ggtatatccc aacccggggt cgggccagat cactgtggaa
     2941 tggccggggc acctgatcaa gaccatgcgt ttcgtggacg cgcagggccg atgcgcatac
     3001 tccatcacct gcgccagctg tgtacgagca gatgtgaacg ttccttcatc cgggctctac
     3061 gtgcttattg tagaggatga gcagggtgat cggatctcca caaaactgac caaaccatga
     3121 tttcgacaag atggtggctc gtgctgccca tactggtgtc atgtaccaag gcaggtgcgc
     3181 aaacctggtg ccccacgggg gccgaatggt ggtatgaaca tcagaacttt ggccaggcag
     3241 gatatgtgca tgcggcctac gtcggcgaca cgctggtcga tggggagcca gcccagcggt
     3301 tggaggcgta cgcgacggga tatgacttct ggatcgacat gccttactac atgcaatacc
     3361 catcggtgat cacccgacag gtcggtggcc aggtgagcta tcgccacaat gaggcatggt
     3421 atacgctgtt cgatctggat gcgcctcccg gtgcgcaatg gccgttggcc ggcttgggtc
     3481 tctttgacga tcgaacgatg gcggtgcttg atacaggcca tttcgtagtg gatggtatca
     3541 tgctgcgatg gtcggaagtg gagttcatcc caccgttcgg gtcatgggct acggacacga
     3601 tagttgagcg agcgggctac aagaatgtgt ttatcgagcc atggcgaagt ttgatgcttg
     3661 aaggcatagt gcaggggctg cgctgctacg ctgacgacgg aattgcgtac agcacgggcc
     3721 ttaccgcaac atgcgatctg atcctgagca cggaagagtt cagcgcccac gggtcatttc
     3781 atgtccatcc caaccctggt tcggactatt tgtgggtgaa gtgcggaagc ccggtccgga
     3841 cggccgaggc tcgcgatctg ttgggccgtt cggtttggag cggtcggggt cctcaggcag
     3901 catcccaggt ggatacctcg tcctggccac caggctgcta cttggtggcg gccgaattcg
     3961 ggaacggcac ccgcgcggtg cgcaaatgga tcaagcaatg acaaggaggt tcgggcttgc
     4021 ggtggtcgcc tatcctgtag gacagtagag gtgttcggcg tacgtcaagc gtccatcgga
     4081 tgccactggc attcacgggc caactgaccg gtcccgcccg ataccggttg ataacccggt
     4141 tgccgcggcc cccggggatc ggctaccttc gcgccgcgaa aaaaaacggg tacgaacgca
     4201 cctacgcctt cgccgggcca cggcgagcaa agcatgagca ccaagaccgc cgaaaagttg
     4261 aagtacaagg tgaaagacat ggccctcgcc gaatggggcc gcaaggagat cgaactggcc
     4321 gaggccgaga tgcccggtct gatggccctg cgcgagaagt acggcaagga gaagccgctg
     4381 aagggcgccc gcatcgccgg ttgcctgcac atgacgatcc agacggccgt gctgatcgag
     4441 accctcaagg agctcggcgc cgaagtgagc tggagcagct gcaacatctt cagcacgcag
     4501 gaccacgccg cagcggccat cgccaaggcg ggcatccccg tgtacgcttg gaagggcatg
     4561 aacggcgaag agttcgattg gtgcatcgag cagacgcttt tcgccttcga aggcggcaag
     4621 ccgttgaaca tgatcctgga cgatggtggc gaccttacca acatggtctt cgacaaatac
     4681 cctgaattgg tgaaggacat caagggcctg agcgaggaga ccaccacggg cgtgcatcgc
     4741 ctgcacgaac gcgagaagaa cggaacgctc gtgatgccgg cgatcaacat caacgacagc
     4801 gttaccaaga gcaagttcga caacaagtac ggctgcaagg agagcgcagt ggacgccatc
     4861 cgccgcgcca ccgacgtgat gatggccggg aaggtggcca tcgtgtgcgg ctacggcgac
     4921 gtgggcaagg gcacggcggc ttcgctgcgc ggcgcgggcg ctcgcgtgat cgtcaccgag
     4981 atcgacccga tctgtgccct ccaggcatc
//
LOCUS       DAOQOC010000058         5005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6977_length_5005_cov_3.048687, whole genome shotgun sequence.
ACCESSION   DAOQOC010000058 DAOQOC010000000
VERSION     DAOQOC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6977_length_5005_cov_3.048687"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PL106_01580"
     CDS             <1..676
                     /locus_tag="PL106_01580"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNA31786.1"
                     /translation="MALGGQGAPLVPLGEQLLFPEHKAFLNIGGICNLSLHHGDHVIG
                     YDLCIGNQALNFLAEEAGMPYDMDGVLARSGEVDDGLLARLNALPFHQQPPPRSLGRE
                     WFEGDVRPLIADARMPLPRRMRTAVEHMAQQIGRAMRGHQGPLLVSGGGAHNSFLVER
                     IATHVPVELPPPLVIDYKEALIFALLGVLRMRGEPTALASVTGARQGSSGGAVFEPFA
                     LSTPRA"
     gene            766..1989
                     /locus_tag="PL106_01585"
     CDS             766..1989
                     /locus_tag="PL106_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNA31787.1"
                     /translation="MREALRRFEQKEPEIVFTWTDSPTGARGWTVINSLRGGAAGGGT
                     RMRKGLDRREVESLAKTMEVKFTVSGPAIGGAKSGIDFDPTDPRKREVLERWYKAVTP
                     LLKNYYGTGGDMNVDELHEVIPITERYGLLHPQEGVVRGHFGADDARAQRAIGQLRTG
                     VSKLVDDTAYVPVAGRYAVADMITGWGVAESVNHFYRIHGGHVRGKRVIVQGWGNVAA
                     AAGYYLAQQGARIVGIIDRHGGLLRPDGLGPEEVTTLFLNKNGNTLRSQDQLPFDEVN
                     ARIWDMGAEVFLPCAASRLVTKDQIDRLCKAGLETIASGANVPFADAEIFYGPIAEHA
                     DGLVSVIPDFVANCGMARVFAYCMGHGADLSDRAIFQDVSAVIGSALERVHARHPQRT
                     HITSTAFGIALEQLA"
     gene            2035..2754
                     /locus_tag="PL106_01590"
     CDS             2035..2754
                     /locus_tag="PL106_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNA31788.1"
                     /translation="MHFPILAQITSTQQLRDSLAQAATTAAATPTQDQLTVWDLISMG
                     SWFIMGPLALMSLLTIYIVIERMMVLGKANREDKDFMNKVRDYVHEGKIDSARNLCSQ
                     SAAPSARMVEKGLQRMGKPAKEIESAMETAGRIEITRLEKGSSLLRLIARLAPMFGFI
                     GTIIGVIKIFYDISLTDNISIAVISTGLYQKMVTSAGGLIVGIVAFFGHHLVHSMVDR
                     AMDKMERTSLAFMDLLQEPTK"
     gene            2764..3162
                     /locus_tag="PL106_01595"
     CDS             2764..3162
                     /locus_tag="PL106_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNA31789.1"
                     /translation="MNLRRRHTEDAEVSTESLNDIMFFLLLFFLIISTLANPNVIKLM
                     LPKSANNEQLAKQQVNLGVTKDKQYTINKKPVPFDQLEAAISAEIAGLDQPTVVLHFD
                     RELSVQDLVDVMQIGTKIKVKMVLATQKQS"
     gene            3259..4074
                     /locus_tag="PL106_01600"
     CDS             3259..4074
                     /locus_tag="PL106_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNA31790.1"
                     /translation="MTASAPDNSMQQKSIALGVSITLHALLLLFFLLYKIITPIPPFP
                     DKEGGGAGYELALGFSELGMGDNQDVTDPSASAAQQESTPPPEEQAEVLTSDVDENDV
                     ATPPKKDDKPKTDKSKEKPKPTAEEIAREKQRKIDELMGNKGSGSGGQGANDQPGDAG
                     GASGSPTGTGQGNGTGSYKGDGWAVDLAGRTIRRKPVINDKPSVAGKVVMDIWVDPDG
                     KVTRTSQNTGKSTTLDQTLVTIARRAALESTFYPDPKAVGEKKGTMTFVFVLQ"
     gene            4128..>5005
                     /locus_tag="PL106_01605"
     CDS             4128..>5005
                     /locus_tag="PL106_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNA31791.1"
                     /translation="MTAYQRTLEYLYAQLPMFSRIGAAAYKADLGNTHALMHLLDHPE
                     RDLKCVHVAGTNGKGSTCSLIASTLQEAGYKVGLHTSPHLRDFRERFRVNGTMPSEED
                     ITGFVDHYRAAFEPIAPSFFEWGVAFTLWWFRQQQVDIAVIETGMGGRLDSTNVVSPE
                     VSVITNIGWDHMQFLGDSLQTIAAEKAGIIKPGVPVVLGEDNVEVTPVIERIATEKGS
                     PLIKAHEGVGAILPCGLVGEHQERNKRTAFAAIQVLHAQGWKVDHHATMKGFINVVRN
                     TGLRGRWETLSRDPLT"
BASE COUNT         1034 a         1604 c         1551 g          816 t
ORIGIN      
        1 catggcgctg ggcggccaag gcgcgccctt ggtgccgctg ggcgaacagc tgctcttccc
       61 ggagcacaag gccttcctga acatcggggg catctgcaac ctctcgctcc accatgggga
      121 ccacgtgatc ggctacgact tatgcatcgg gaatcaagcc ctgaactttc tggccgaaga
      181 agccggcatg ccctacgaca tggacggggt gctggcccgc tccggtgagg tggacgatgg
      241 gttgctggcc cggctgaatg ccctgccctt ccaccagcag ccacctccgc gctccctggg
      301 tcgtgagtgg ttcgaggggg atgttcggcc cctgatcgct gatgcacgca tgccgctccc
      361 tcggcgcatg cgcaccgcgg tggagcatat ggcccaacag atcgggcggg cgatgcgcgg
      421 gcatcaaggc cccctgctgg tgagcggcgg cggggcgcac aacagcttcc tggtggaacg
      481 catcgcgaca cacgtgcccg tagagctgcc ccctccgctg gtgatcgact acaaggaggc
      541 cttgatcttc gccttgttgg gtgtgctgcg gatgcgtggt gaacccaccg cgctggcgag
      601 cgtgaccggg gcccgtcagg gcagctcggg cggggccgtg ttcgagccct tcgcgctttc
      661 aacaccccgc gcatgaaaac cccgccatgc ccgggaaacc cccaacaagc ccgcctctac
      721 ttttgatccg ccatgaaggg aacccagcca ttggccgctg aagggatgcg cgaggccttg
      781 cgccgctttg agcagaaaga gccggagatc gtcttcacct ggaccgacag tcccaccggc
      841 gcacgcggtt ggaccgtgat caacagcctg cgcggtggtg cggcgggtgg cggcacacgc
      901 atgcgcaagg gcctcgaccg ccgcgaggtg gaaagcctgg ccaagaccat ggaggtgaag
      961 ttcaccgtga gcgggcccgc catcggcggc gccaagagcg gcatcgactt cgaccccacc
     1021 gatccgcgca agcgcgaagt gctcgaacgc tggtacaagg ccgtgacacc cctgctgaaa
     1081 aactactacg gtaccggtgg cgacatgaac gtggacgagc tgcacgaagt gatccccatc
     1141 accgagcgct acggcctgct gcacccccag gagggcgtgg tacgcggcca cttcggcgcc
     1201 gatgacgcgc gggcacagcg cgccatcggc cagttgcgca ccggtgtgag caagctggtg
     1261 gacgacaccg cctatgtgcc cgtggccggc cgttacgccg tggccgacat gatcacgggc
     1321 tggggcgtgg ccgagagcgt gaaccatttc taccggatcc acgggggcca tgtacggggc
     1381 aagcgcgtga tcgtgcaggg ctggggcaat gtggccgcag cggccggata ctacctggcc
     1441 cagcaaggcg cgcgcatcgt gggcatcatc gaccggcatg gtgggctgct acgtcccgac
     1501 ggcctgggcc ctgaggaggt caccaccctc ttcctcaaca agaacgggaa caccctgcgg
     1561 agccaggacc agttgccctt tgatgaggtg aacgcgcgga tctgggacat gggcgcggag
     1621 gtcttcctcc cctgcgcggc ctcgcgcctg gtgaccaagg accagatcga tcgcttgtgc
     1681 aaggccgggc tggagaccat cgccagtggc gccaacgtgc ccttcgccga tgcggagatc
     1741 ttttacggcc ccatcgccga acacgccgat ggcctggtga gcgtgatccc cgacttcgtc
     1801 gccaattgcg gcatggcccg cgtgttcgct tactgcatgg gccatggggc cgacctcagc
     1861 gaccgggcca tattccagga cgtcagtgcc gttatcggtt cggccctgga acgggtgcat
     1921 gccaggcacc cgcaacgcac gcacatcacc agtaccgcct tcggcatcgc actcgaacaa
     1981 ctcgcctgaa atttcccgga cggcttcacc gttccacagt aacaacccgc actgatgcac
     2041 ttcccgatcc tcgcgcagat caccagcacc caacagctac gcgactcgct cgcccaagcc
     2101 gccaccacgg cggccgctac gcccacacag gaccagttga ccgtttggga cctgatcagc
     2161 atgggcagct ggttcatcat ggggcccctg gccctgatga gcctgctcac catctacatc
     2221 gtgatcgaac ggatgatggt gcttggcaag gccaatcgcg aggacaagga cttcatgaac
     2281 aaggtgcgcg actacgtgca cgaagggaag atcgacagcg cccgcaacct ctgctcgcaa
     2341 agcgccgctc ccagcgcacg catggtggag aagggcctgc agcgcatggg caagcccgcc
     2401 aaggagatcg agagcgccat ggaaacggcc gggcgcatcg agatcacccg gctggagaag
     2461 ggatcctcgc tgctgcgcct gatcgcccgc ctcgcgccga tgttcggctt catcggcacc
     2521 atcatcggtg tgatcaagat cttctacgac atctccctca ccgacaacat cagcatcgcc
     2581 gtcatcagta cgggtctcta ccaaaaaatg gtgaccagcg cgggcggcct catcgtgggt
     2641 atcgtcgcat tcttcggtca ccacctggta cacagcatgg tggaccgcgc catggacaag
     2701 atggagcgca ccagcctggc cttcatggac ctgctgcagg aaccgaccaa ataagccagc
     2761 gcgatgaacc tgcgccgcag acataccgag gatgccgagg tcagcaccga gtcgctgaac
     2821 gacatcatgt tcttcctgct cctgttcttc ctgatcatca gcacgctggc caaccccaac
     2881 gtgatcaaac tgatgctgcc gaaaagtgcc aacaacgagc agctggccaa gcagcaggtg
     2941 aacctgggtg tcaccaagga caagcagtac accatcaaca agaagccggt gcccttcgat
     3001 cagcttgagg cggccatttc ggcggagatc gccggcctgg accagcccac cgtggtgctg
     3061 cacttcgacc gcgaactcag cgtgcaggac ctggtggacg tgatgcagat cggcacgaag
     3121 atcaaggtga agatggtgct ggccacgcag aagcagagtt gaaccatgta tgccgtgcca
     3181 ggatgtgatg ttggttggga gaacgaacgt ggcctcactc ttgtacccca gctcgtacgt
     3241 tgaccaaacc gcaaggccat gaccgcatcg gcaccggaca acagcatgca acagaagagc
     3301 atcgccctgg gcgtgtccat cacgctgcac gcgttgctcc tcctgttctt cctgctctac
     3361 aagatcatca cacctattcc cccgttcccc gataaagaag gcggtggtgc cgggtatgag
     3421 ctggcactgg gcttcagcga actgggcatg ggcgacaacc aggacgtgac cgaccccagt
     3481 gcgtcagcgg cccaacagga aagcacaccg ccgcccgagg aacaggccga agtgctcacc
     3541 agcgatgtgg atgagaatga cgtggccacg ccaccgaaga aggacgacaa gcccaagacc
     3601 gacaagtcca aggagaagcc gaaacccaca gcagaggaga tcgcccgtga aaagcagcgc
     3661 aagatcgacg agttgatggg caacaagggc agcggctcag gtgggcaggg cgccaatgat
     3721 caacccggtg atgccggcgg tgccagcggc agtcccacag gaacaggtca ggggaatggt
     3781 accggatcgt acaaaggtga tggctgggcc gtggacctcg ccggtcgcac gatcaggcgc
     3841 aaaccggtga tcaacgacaa acccagcgtg gccggcaaag tggtcatgga catctgggtg
     3901 gatccggatg gcaaggtgac ccgcaccagc cagaacaccg gcaaaagcac cacactcgat
     3961 cagaccctgg taaccatcgc ccggcgtgcc gccctcgaaa gcacctttta tcccgatccc
     4021 aaggccgtgg gagaaaagaa gggcaccatg accttcgtct tcgtactgca ataggtggaa
     4081 ccgccacgtc gcttgttgtc caacaggtat cacggtccca ttgcgcgatg accgcctacc
     4141 agcgcacctt ggaatacctc tatgcgcaac ttccgatgtt ctcgcgcatc ggagctgcgg
     4201 cgtacaaagc cgacctgggc aacacgcacg cgctcatgca cctgctcgat catcccgagc
     4261 gcgacctgaa gtgcgtacac gtggccggga cgaacggcaa gggcagcacc tgctcgctga
     4321 tcgcgagcac gctgcaggaa gccgggtaca aggtgggcct gcacacctcg ccgcatctca
     4381 gggacttccg cgagcgcttc cgcgtgaacg gtaccatgcc atctgaggag gacatcaccg
     4441 gtttcgttga tcactatcgc gcggccttcg aaccgatcgc accctccttc ttcgaatggg
     4501 gtgtggcctt caccctgtgg tggttccgcc agcaacaggt ggacatcgcg gtgatcgaaa
     4561 ccggcatggg cgggcgcctg gacagcacca acgtggtgag cccggaggtc agtgtgatca
     4621 ccaatatcgg ctgggaccac atgcaattcc tgggcgattc gttgcagacg atcgcagcgg
     4681 aaaaagccgg catcatcaaa ccgggggtgc ctgtggttct gggcgaagac aatgttgagg
     4741 tcactcctgt tatcgaacgc attgcgacgg agaagggctc gccgctgatc aaggcccacg
     4801 agggtgtcgg tgccatcctc ccctgtggcc tggtcggcga gcaccaggag cgcaacaagc
     4861 gcacagcctt cgccgcgatc caggtcttgc atgcgcaggg ttggaaggtg gaccaccatg
     4921 ccacgatgaa gggcttcatc aacgtggtgc ggaatacggg gctgcgcggg cgttgggaaa
     4981 cactttctcg cgatccgctc actat
//
LOCUS       DAOQOC010000059         4997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_6999_length_4997_cov_3.625253, whole genome shotgun sequence.
ACCESSION   DAOQOC010000059 DAOQOC010000000
VERSION     DAOQOC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6999_length_4997_cov_3.625253"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="purB"
                     /locus_tag="PL106_01610"
     CDS             <1..965
                     /gene="purB"
                     /locus_tag="PL106_01610"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNA31792.1"
                     /translation="KDFVFAHHVPQLTALHDRLHGLALDWAQVPMLARTHGQPASPTR
                     LGKEIEVFVERLDVQLRTLRAVPFTCKFSGATGNFNAHHAAYPELDWPKLADRFCEEK
                     LGLKRQRFTTQIEHYDQMAALFDAMRRVNTILIDLCRDVWTYISLDYFKQRTRPGEVG
                     SSAMPHKVNPIDFENAEGNLGMANALLGHLSEKLPVSRLQRDLTDSTVTRNIGVPMAH
                     VHIAVDAIMNGLDKLLLNEEALRRDLDAQWAVVAEGIQTILRREGYPEPYERLKDLTR
                     GKARIGQEDLAAFIGKLDVSEDVKARLRTLTPHNYTGVDLIGRG"
     gene            complement(962..1528)
                     /locus_tag="PL106_01615"
     CDS             complement(962..1528)
                     /locus_tag="PL106_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNA31793.1"
                     /translation="MSPPVGENAYFQHVCQPVFNSAPFMDITIVIRQYAEYDRWASTR
                     IADRLAREPDGLLDTPLQSSFPSLRATLMHVRNAHAAWFQRMSGIPQQWPAEPSDELG
                     TFLKHVNVMCGYATTLPLEALMGDATYTDLKGNAHTTPRWQPLMHCFNHGSYHRGQIV
                     SMMRQLGLDDIPATDLVVYQRLLKKGGA"
     gene            1565..2110
                     /locus_tag="PL106_01620"
     CDS             1565..2110
                     /locus_tag="PL106_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31794.1"
                     /translation="MISTLFGRKRITEEKLANAVVGRVFDLTENAYPLVVEELQDAPE
                     FTVRPVFGPGDDELFALIILAGNLLDAPKHMPAGQDRRFVAHAISKYGAAMDIPASEL
                     ETEVIALERFMERVNHPSKNTVYAMSKTIFHKYDLFRFQDTYFRSMKAPNPIVLSRLN
                     KLMAFCLWDWSEVLDGWKVTQ"
     gene            complement(2142..3362)
                     /gene="lhgO"
                     /locus_tag="PL106_01625"
     CDS             complement(2142..3362)
                     /gene="lhgO"
                     /locus_tag="PL106_01625"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNA31795.1"
                     /translation="MAEDITWDAIIVGGGIVGAATFFKLQSRFPQKRLLLIEKEPVLS
                     DHQTGHNSGVIHSGIYYKPGSYKAKNCVEGRRELVAFAKQHGIAHDVCGKLIVAMEPD
                     ELPRLDKIHERGLANGIEDMERIGPERIKEIEPFCTGIAGIRIGCTGIIDFRGATNKM
                     AELACGINPASAVHTGEEVRTSVERGEVSIVRTNKGTHKARAVIFCAGLQSDRMARND
                     GARVNERIVGFRGDYYELTPQAKHKVKHLIYPVPDPRFPFLGVHFTRMTDGSIECGPN
                     AVFTFKREGYGKTDFSLADTWDALTYGGTWKLFFKNWRYGLDEYRRAFSKKLFLRTLR
                     RLIPSLTMDDIEPGRAGVRAMLLGTDGEMVDDFRIERRGRHIHVLNAPSPAATACLAI
                     GNEIVNMAVKEGVI"
     gene            complement(3382..4689)
                     /locus_tag="PL106_01630"
     CDS             complement(3382..4689)
                     /locus_tag="PL106_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA31796.1"
                     /translation="MIVKGDKKVIRAWTMYDWANSAYSLTITSAVFPAFYVATTMDGP
                     KDLVLERFGVRAAAAYPWSLSVGFLIVALIAPILSGIADHRGNKKSFLKFFCYLGAAS
                     CAGLAFFSLDTFWIGLACVALACIGFSGSLVFYDAYLPEIADPADHDRVSARGYTMGY
                     IGSVILLVLNLATVLAWPDESWPARLSFISVGLWWAGWAQIAFARLPQGSRTADDVNV
                     LTNGYKELRKTWIELGRTIRLKRFLLAFFVLNMGIQTVMYLAANFAKQEVKELDATGA
                     EVPISDASLITAILIIQLVAILGAVTFVRLSSRFGNVRALMIGAVVWVLACLFAYSLH
                     WAVEFYLLAGVVGLVMGGSQALARSTYAKFLPDTDDHASYFSFFDVSYYLGTVLGTAA
                     YGAVYAITGDLRHTVIAIACFFILGFCLLWRVPRNERTLPAMR"
     gene            complement(4766..>4997)
                     /locus_tag="PL106_01635"
     CDS             complement(4766..>4997)
                     /locus_tag="PL106_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA31797.1"
                     /translation="LVGHTDAIEADRVRMRPDLTDLDKERAETVKQALVEAGIDAARI
                     TTAGLKHESPADAGDDEVALAKNRRVEVELEQ"
BASE COUNT          969 a         1557 c         1586 g          885 t
ORIGIN      
        1 tgaaggattt cgtgttcgcg catcatgtgc cgcagctcac ggccctgcac gaccggctgc
       61 acggcctcgc gttggactgg gcccaggtgc ccatgctggc gcgcacgcac ggccagcccg
      121 ccagccccac acgcttgggc aaggagatcg aggtcttcgt ggagcgcctc gatgtgcaac
      181 tccgtacgtt gcgcgccgtg cccttcacct gcaagttcag cggggccacg ggcaatttca
      241 atgcgcacca cgcggcgtac cccgaactgg actggccgaa actggccgac aggttctgcg
      301 aggagaaact cggcctcaag cgccagcgct tcaccacgca gatcgagcac tacgaccaga
      361 tggccgcgct cttcgacgcc atgcgccggg tgaacaccat cctgatcgac ctgtgccgcg
      421 acgtgtggac gtatatcagc ctggactact tcaagcagcg gacccggccg ggcgaagtgg
      481 gcagcagcgc catgccccac aaggtgaacc ccatcgactt cgagaacgcc gaagggaacc
      541 tgggcatggc caatgcgctg ctcgggcacc tgagcgaaaa acttccggtt tctcggttgc
      601 agcgcgacct taccgacagt acggtaacgc gcaacatcgg cgtgcccatg gcccacgtgc
      661 atatagccgt ggacgcgatc atgaacgggc tggacaagct cctgttgaac gaggaggcgc
      721 tgaggcggga cctcgatgcg cagtgggccg tggtggccga aggcatccag accatcctcc
      781 ggcgtgaagg ctatcccgag ccctatgagc gactgaagga cctcacgcgc ggcaaggcac
      841 gtatcggcca ggaggacctc gccgccttca tcgggaaact cgacgtgagc gaggacgtga
      901 aggccaggct gcgcacactc accccccaca actacacggg ggtggacctg atcggacggg
      961 gttaggcccc gcctttcttc agcaggcgct ggtacacgac aaggtccgtg gccgggatat
     1021 cgtccagacc gagttgccgc atcatgctca cgatctgacc ccgatgatag ctaccgtgat
     1081 tgaagcagtg catgaggggc tgccagcggg gcgtggtatg cgcattcccc ttcagatcgg
     1141 tgtaagtcgc gtcgcccatg agcgcttcga gcggcaaggt cgtcgcgtac ccgcacatca
     1201 cgttcacgtg cttgaggaaa gtgcccagtt catccgacgg ttcggcgggc cactgctgcg
     1261 ggatgccgga catgcgctgg aaccaggccg catgggcatt gcgaacgtgc atgagcgtgg
     1321 cccgcaggga cgggaagctg ctttgtagcg gtgtatccag caggccgtcg ggttcgcgtg
     1381 ccagtcgatc agcgatgcgc gtactggccc aacgatcgta ctcagcgtac tggcgaatga
     1441 cgatggtgat gtccatgaac ggggcggaat tgaacacggg ttgacaaacg tgttgaaaat
     1501 aggcgttctc accgaccgga ggcgacatgc gcacccgggt actttcgcca gcagaaccct
     1561 gacgatgatc tcgacactgt tcggccgcaa gcgcatcacc gaggagaaac tggcgaatgc
     1621 cgtggtaggc cgggtcttcg atctcacgga gaatgcgtac cccctggtgg tggaggaatt
     1681 gcaggacgcc ccggagttca cggtgcgccc cgtgttcggc ccgggcgacg atgagctctt
     1741 cgcgctgatc atactcgccg gcaacctgct ggatgcgccc aagcacatgc ccgctgggca
     1801 ggaccgccgc ttcgttgcgc acgcgatcag caagtacggc gccgcgatgg atatcccggc
     1861 gagcgaattg gaaacggagg tgatcgcgct ggaacgcttc atggagcggg tgaaccaccc
     1921 gagcaagaac acggtgtacg ccatgagcaa gacgatcttc cacaagtacg acctgttccg
     1981 attccaggac acctacttcc gctccatgaa ggcgccgaac ccgatcgtgc tcagccgctt
     2041 gaacaagctg atggccttct gcctgtggga ttggtcggaa gtgctcgacg gctggaaggt
     2101 cacgcagtag ctcagccact ggcttttggc tactggtgca ttcagatcac gccttccttc
     2161 accgccatgt tcacgatctc gttgccgatg gccagacagg ccgtggccgc aggcgagggt
     2221 gcattgagca catggatatg acggccacgc cgctcgatgc ggaagtcgtc caccatttcg
     2281 ccatcagtac ccagcagcat ggcccgcacc cctgccctgc cgggttcgat gtcgtccatg
     2341 gtgagtgaag ggatcagccg ccgcagtgtt ctcaggaaga gtttcttgct gaacgccctc
     2401 cggtactcat cgagtccgta gcgccagttc ttgaagaaga gtttccaggt gccgccgtag
     2461 gtgagcgcgt cccacgtgtc ggccaggctg aagtcggtct tgccgtagcc ctcgcgcttg
     2521 aaggtgaaga cagcgttggg cccgcactcg atgctgccgt cggtcatgcg ggtgaaatgc
     2581 acaccgagga aggggaagcg cggatcgggc acggggtaga tcaggtgctt caccttgtgc
     2641 ttggcctgcg gggtgagttc gtagtaatcg ccacggaagc ccacgatccg ctcgttcaca
     2701 cgggccccgt cgttgcgcgc catgcggtcg ctttgcagcc cggcgcagaa gatcaccgcg
     2761 cgggccttgt gcgttccctt gttcgtgcgc acgatactca cctccccgcg ttccaccgaa
     2821 gtccgtacct cttcgccggt atgcactgca ctggcaggat tgatcccgca cgccagttcg
     2881 gccatcttgt tcgtggcacc gcggaagtcg atgatgccgg tgcagccgat gcggatgccc
     2941 gcgatgcccg tgcagaacgg ctcgatctcc ttgatgcgct ccggtccgat gcgctccatg
     3001 tcctcgatgc cattggccag tcctcgttca tggatcttgt ccagccgggg cagttcatcg
     3061 ggctccatgg ccacgatcaa ctttccgcag acatcgtggg cgataccgtg ctgcttcgcg
     3121 aaggccacga gttcgcggcg gccctccacg caattcttcg ccttgtagct gccaggcttg
     3181 taatagatac cgctgtggat cacaccactg ttatggccgg tctggtggtc gctcaggaca
     3241 ggctccttct cgatcagcag cagccgcttc tgcgggaagc gcgattggag tttgaagaag
     3301 gtggcggcgc ccacgatgcc gccgcccacg atgatcgcgt cccaggtgat gtcctcagcc
     3361 atacggccgc gaagatcgtg ctcagcgcat tgccggaagc gtccgttcgt tccgtggcac
     3421 acgccagagc aggcagaacc ccaggatgaa gaagcaggcg atggcgatca ccgtgtgacg
     3481 cagatccccg gtgatggcgt acaccgcacc ataggccgcc gtgcccagca cggtgcccag
     3541 gtagtagctc acgtcgaaga agctgaaata gctcgcgtgg tcatcggtat cgggcaggaa
     3601 cttggcgtag gtgcttcggg ccagggcctg cgacccgccc atcaccaggc ccaccactcc
     3661 ggccagcagg tagaactcga cggcccagtg cagcgaatag gcgaagaggc aggcgagcac
     3721 ccagaccacc gcgccgatca tgagcgcacg cacgttgccg aagcgggaac tgaggcgtac
     3781 gaaggtgaca gcgcccagga tggccaccaa ctggatgatg agaatagccg tgatcaggct
     3841 ggcgtcggag atggggacct ccgcgccggt ggcgtccagt tccttcacct cctgtttggc
     3901 gaagttggcg gccaggtaca tcacggtctg gatccccatg ttgagcacga agaaggccag
     3961 gaggaatcgc ttgagccgga tggtacggcc cagttcgatc caggtcttgc gcagttcctt
     4021 gtatccattg gtgagaacgt tcacgtcgtc cgccgtgcgg gaaccctgtg gcaagcgcgc
     4081 gaaagcgatc tgcgcccaac cggcccacca gagacccacg gagatgaagc tcaggcgcgc
     4141 gggccaactc tcgtcgggcc aggccagcac ggtagccagg ttcagcacaa gcaggatcac
     4201 actgccgatg tagcccatgg tatagccccg cgcgctcacc cggtcgtggt cggcgggatc
     4261 cgcgatctcc ggcaggtagg catcgtagaa gaccaagctg ccgctgaaac cgatacaggc
     4321 cagtgccacg cacgccagtc cgatccagaa cgtatcgagc gagaagaagg cgaggccggc
     4381 acaactggcg gcgcccaggt aacagaagaa cttcaggaag gacttcttgt taccacggtg
     4441 gtcggcgata ccggaaagga taggtgcgat gagggccacg atgaggaagc cgaccgacag
     4501 tgaccaaggg taggccgctg cggcacgaac gccgaagcgc tccagcacca gatccttcgg
     4561 accgtccatg gtggtggcca cgtagaacgc cggaaagacg gccgaggtga tcgtgagcga
     4621 gtacgccgag ttggcccagt cgtacatggt ccaggcgcgt atgaccttct tgtcgccttt
     4681 gacgatcatg ggggcaaagt agaccgggaa aagcgaaggc ccggaggatc gatccgccgg
     4741 gccttcgctc catgccgtgc cagggtcact gttccagttc cacctccacc ctcctgttct
     4801 tggccagggc cacctcgtcg tctccggcat cggcggggct ctcatgcttc aggccggccg
     4861 tagtgatccg cgcagcatca ataccagctt ccaccaaggc ttgcttcacg gtctcggcgc
     4921 gttccttgtc gaggtcggtg aggtcggggc gcatgcgcac gcgatcagcc tcgatggcat
     4981 ccgtatggcc caccagg
//
LOCUS       DAOQOC010000060         4993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7006_length_4993_cov_3.765897, whole genome shotgun sequence.
ACCESSION   DAOQOC010000060 DAOQOC010000000
VERSION     DAOQOC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_4993_cov_3.765897"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1899
                     /locus_tag="PL106_01640"
     CDS             175..1899
                     /locus_tag="PL106_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNA31798.1"
                     /translation="MDGSSDAYLVEFDPDATQLEGTYYGGNATDEGRGCGTNVMGDVF
                     LVGYTYSPDSISFGNAHQDELSGSPDWFIAMFSDTVDCAGVPGGNGLPGTSCDDASAC
                     TIEDAYTANCTCVGTELVAGSIAGSQQVCVEGTATLTISGNNAPGTWSSTDPGVASVN
                     SSGVVTGVALGTVFIRFTVCPGIFVLHMVSVFAPPDAGTNGALTLCATGGQQSLLAQL
                     GGTPDPGGTWSGPSPVTDNSYDPLTMEPGTYTYTVAATSPCTGDATAQVVVAEDVITT
                     WYSDSDGDNFGDPSDSLMTCTPPQNYVLNNTDCDDDDNTVWQNEVLFVDADGDGYDAG
                     SDTICHGASIPPGFSVASNGTDCSDSDNLITGVGTTCDDDDPGTCNDVINVACVCAGT
                     EVVQFGTISGDNTINEGQSASYTYGPLLANAVFLWTSDPLVTVIAGQGSPHVVINVPL
                     GVWGNVAELCCSVSVPDCGQDTTLCMDVYVSDVVGLQEAFDAGGTWFSVRPNPGEGQF
                     ELIPHRAFSGPVRYELRDVAGRSMGPERLITGPGPVMFDLRPSPPGVYFLRMRSEGEV
                     QVLRLVVR"
     gene            2081..2491
                     /locus_tag="PL106_01645"
     CDS             2081..2491
                     /locus_tag="PL106_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31799.1"
                     /translation="MRVEVDHVEVPLNGATAVPVDTIWHPITVQHPLVLESGDRVVAV
                     LEVGLGSNPCTQAPLVEIRTLLSGQGNGCGVYWPPQEVDHQFTCTTEFPVVPDLVGEQ
                     TIQAQARVTGGVCDSVDCALNNRSLNLLIIHEHP"
     gene            complement(2721..4739)
                     /locus_tag="PL106_01650"
     CDS             complement(2721..4739)
                     /locus_tag="PL106_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA31800.1"
                     /translation="MWLNSRGLDSMLMPLVACLLGQVPTNAQVNQLTIEDKVKINIAA
                     ESQVYSYVSILNAISRNDAGSASAVEDLKQYVMAITTGDERIFTDSTALIEDNINTTF
                     TADKPVSNLTVPVYFTDFLNQFQRDTTERPGQHTEPITMVRADYGEPHTTADGLVVTS
                     LLYNVNWHGTQRISHAPYAPQQRVILFKAERKDAHDWAVRIAVDGWYAPGSEPFQSFE
                     KELEAEQARSKDQKNTRLQAYRAAAAAALEQARFAREQRTAVYQDHINRGDQQLGTDP
                     ENALFWYGKAGEAAPAGRWDHVSGQNRARRALEDRARTLQDQALKVMAQSLARQLDAK
                     KYRSVAVLEFTEQDGRPSATGKSLAEKLVIHLSQASIGFNVADQSLVDRALEQRRVVG
                     SIDEKDQIAVGKAANAQAVITGKLIRMGADQVDLTTKIQDIKQRSVAGGGNGMMPILP
                     QDGGSTSVQESKPIDTEPVADRTIKRGHTTLGATAGANLTRLSSTDSLTERFTQGGTG
                     FRVGLEMERMGPKGNARFATGVRLSRYDASTGDEGLARTIQVNYLQLPLLFRLFTNHT
                     SAGRFGLQIGVLGGYYFFGRTESYTLGRYIEVSPFNLHGSAGPCYELALGQGRTRIVA
                     QATYDPMLFGLGNAENNTLTAPANAPRFSAISVAAGATFLISAPGRTF"
BASE COUNT          907 a         1423 c         1586 g         1077 t
ORIGIN      
        1 gtcaagttcg atccagcagg cggcgccggt tcacggagct ggggaagacg ttttggcggt
       61 ccgttcgatg atgatgcgaa ggggtgctcg ttcgatgcgg aggggcacct gttggtgaca
      121 ggaaccacga catcgggttc cgggatcgcg atggttggtg catcccaatc ggtcatggat
      181 ggatccagtg atgcctactt ggttgaattc gatccggatg ccacgcaact cgagggtacg
      241 tattacggcg ggaacgcgac tgatgagggc agaggttgcg gcacgaacgt gatgggggat
      301 gtgttccttg tcggctatac gtactccccc gattccattt cgttcggtaa cgctcaccag
      361 gatgaattat ccggctctcc ggattggttc atcgccatgt tcagcgatac ggtggattgt
      421 gcgggagtac ctggtggaaa tggtctgccc gggaccagtt gcgacgatgc gagcgcgtgt
      481 acgatcgagg atgcctacac agccaattgc acctgcgtcg gcaccgagct tgtcgcggga
      541 tccatcgctg gctcccaaca ggtctgtgtg gaagggacag caacgctcac gatcagcggc
      601 aacaatgcac cggggacgtg gagcagtacc gatccagggg tcgcatccgt gaacagcagt
      661 ggtgtggtga caggggtggc cttgggcacg gtgttcatcc gattcaccgt gtgtccggga
      721 atattcgtac tgcacatggt gagcgtattt gcgccacccg atgccggtac caacggcgca
      781 ctcaccctat gcgctaccgg aggacagcag agtcttcttg cccaactggg cggtaccccg
      841 gatccagggg gcacgtggtc cggtcccagt ccggtcaccg acaacagcta tgatccgctc
      901 acgatggagc cgggcaccta tacatatacc gtggccgcca caagcccgtg tacaggtgat
      961 gccacggccc aggtagtggt cgccgaagat gtcataacaa cgtggtattc ggatagtgac
     1021 ggtgacaact tcggtgatcc ctccgacagc ttgatgactt gcacgccacc tcagaactac
     1081 gtgctcaaca acaccgactg tgatgatgac gacaacaccg tctggcagaa cgaggtcttg
     1141 ttcgttgatg cggatggtga tgggtatgat gcaggcagcg atacgatctg tcatggggca
     1201 tcaattccac ctggattcag cgtcgcatcg aatggtaccg actgcagcga ctcggacaac
     1261 ttgatcaccg gggtcggcac aacttgtgat gacgatgatc ccggcacgtg caacgatgtg
     1321 atcaacgtgg cttgtgtttg cgctggtacc gaagtggtcc aattcggcac gatctcaggt
     1381 gacaacacca tcaatgaggg gcagtcggca tcatacacct acggtccgct gctcgccaac
     1441 gctgtgttcc tgtggacatc ggatcccctt gtcacggtca ttgctggaca gggttctccg
     1501 cacgtggtga tcaatgtgcc gttgggtgtt tgggggaatg tggctgagct gtgctgttcg
     1561 gtcagtgtgc ctgattgtgg acaggatacc acactgtgca tggacgtgta cgtcagcgat
     1621 gtggtgggcc ttcaggaagc cttcgatgct ggcggaacgt ggttctccgt gcggccgaat
     1681 cccggtgaag gccagttcga gttgataccc cacagggcgt tctccggtcc ggtgcgttac
     1741 gaactccggg atgtggccgg tcgttcgatg ggacccgaac gcctcatcac cgggcccggt
     1801 ccggtcatgt tcgatctgcg tccgtcaccc cctggtgtct acttcctgcg catgcggagc
     1861 gaaggcgagg tgcaggtgtt gcgtttggtg gtgcggtagt ggtacaggtg tcgttggaac
     1921 cccgcgttga gaatgcgatg atcacgaaaa tactgataat ttggtcttga tcatgagagc
     1981 aagatctctt tcttccattg gttgcccggc gctggttgtt gtgttgttgg gtgcatgcga
     2041 ggatgcgcgt gaagaagagg aggttgatgg accggatacc atgcgggtcg aggtggatca
     2101 cgtggaggtg ccattgaatg gggccactgc ggtccctgtg gacacgatct ggcatccgat
     2161 cacggtacaa cacccgttgg tccttgaaag cggcgatcgg gtcgttgccg ttttggaagt
     2221 gggcctgggt tccaacccgt gcacacaagc cccactggtc gagatccgta cgctcctttc
     2281 gggacaaggg aacggatgtg gggtgtactg gccgccccag gaggtcgacc atcagttcac
     2341 ctgcactacg gagttcccgg tggttcccga tctggtcggc gaacagacca tccaagcgca
     2401 agcccgggtc acgggtggcg tgtgcgacag cgtggattgc gcgctgaaca accggtcgct
     2461 caacctcctg atcatacacg aacacccgta ataccatttc tacattcaag accggacaag
     2521 agatgcgccg cgatcattcg gaggacgata cgctgagcag gtcgcgtagt gggtctccga
     2581 cgcgccataa ccgctttggc gacggagcgg cctatgggac ctgctctgcg gagaaggcat
     2641 ccggagagtg tttcatcccg cttgcatgaa gcgcaagcag ctcggctggt aggaatgtag
     2701 agttggcata aggatcgacc tcagaaggtg cgaccgggag cggagatcag gaaggtggcg
     2761 cccgctgcga cggagatcgc gctgaaacga ggtgcgttcg caggcgcggt cagtgtgttg
     2821 ttctccgcat tgcccaggcc gaagagcatg ggatcgtacg tggcctgggc cacgatccgt
     2881 gttcgcccct ggcccaacgc gagttcgtag cacggtccgg ccgaaccatg cagattgaac
     2941 ggactcacct cgatgtagcg ccccagggtg tagctctcgg tacgcccgaa aaaatagtac
     3001 ccgcccagca cgccgatctg cagtccgaag cggccagcgg aggtgtggtt cgtgaagagc
     3061 cggaacaaca ggggcagttg gaggtagttc acctggatcg tacgggccag gccttcatca
     3121 ccggtggagg cgtcgtaacg cgataggcgc acgccggtcg cgaagcgggc attgcccttg
     3181 gggcccatgc gctccatttc gagcccgaca cgaaaaccgg tgccgccctg cgtgaagcgt
     3241 tccgtcagcg agtcggtgct ggagagcctg gtgaggttcg cccctgcggt ggcaccaagg
     3301 gtggtgtggc cgcgcttgat ggtccgatcg gccacgggct cggtatcgat gggcttcgac
     3361 tcctgaacac tggtgctacc cccatcttgg ggcaggatgg gcatcatccc gttgccacct
     3421 ccggccaccg atcgctgctt gatatcctgg atcttcgtgg tcagatccac ctgatccgcg
     3481 cccatgcgga tcaatttacc ggttatcacg gcctgggcat tggcggcttt gcccacggcg
     3541 atctggtcct tctcgtcgat gctcccgacc accctgcgct gctcgagggc gcgatccaca
     3601 aggctctggt ccgccacatt gaagccgatg ctcgcttgag agaggtggat gaccagtttt
     3661 tccgcgaggc tcttgcccgt tgcgctgggt cgtccgtcct gctcggtgaa ttcgagcacg
     3721 gccaccgatc ggtatttttt ggcatcgagc tggcgggcca ggctctgtgc catcaccttc
     3781 agggcttgat cctggagggt ccgggcccgg tcctccaggg cgcgacgtgc ccggttctgg
     3841 ccactcacat ggtcccatcg gccagccgga gcggcttcac cggctttgcc gtaccagaac
     3901 agggcgttct cggggtcggt gcccagttgt tgatcccccc ggttgatgtg gtcctggtac
     3961 actgccgtgc gctgctcgcg cgcgaaccgg gcctgttcaa gcgctgccgc ggcggcggcg
     4021 cgataggcct gcaaccgggt attcttctgg tccttggaac gggcctgttc ggcctccaat
     4081 tccttctcga agctctggaa cggttcgctg cccggggcgt accaaccgtc caccgcgatc
     4141 cgcaccgccc aatcatgcgc gtcctttcgt tcggctttga agaggatgac acgctgttgt
     4201 ggtgcatagg gcgcatgtga gatccgctgt gtgccatgcc agttcacgtt gtacagcaaa
     4261 ctggtcacca caaggccgtc cgcagtggtg tgcggctcgc catagtcggc tcgcaccatg
     4321 gtgatcggtt ccgtgtgttg gcccggccgt tcagtcgtat cccgttggaa ctggttgagg
     4381 aagtcggtga aatagaccgg aacggtcaaa ttgctcacgg gcttgtcggc cgtgaacgtg
     4441 gtgttgatgt tgtcctcgat caaggccgta ctgtccgtga atatgcgttc gtcaccggtg
     4501 gtgatcgcca tcacgtactg tttcaggtcc tcgaccgcac tcgcgcttcc cgcatcattg
     4561 cggctgatcg cgttcaggat actgacataa ctgtacacct gcgactcggc ggcgatattg
     4621 atcttgacct tgtcctcgat ggtcaattgg ttcacttgcg cattcgtcgg aacttggccc
     4681 agcaggcaag cgaccaaggg catcagcatg ctgtccagcc cacgcgaatt gagccacatc
     4741 ttccggggtc ggggtgttcg acggagcatc ggtctgcaag ccacggtcgg atgggggcgg
     4801 ggtgagcgaa tgtaggattt cgcccccatg tacgggatga tccttataaa ttccgcaccc
     4861 cgaagaaccg atcgccatgc tccgccccac ttccctcgtc ctgtcaacgc tggtcgccac
     4921 gctctgtgcc gcgcagtgtg tcacctcctt cccctccacg cagaacttca ccagtggtac
     4981 cgtgggcacc ccg
//
LOCUS       DAOQOC010000061         4985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7030_length_4985_cov_3.727992, whole genome shotgun sequence.
ACCESSION   DAOQOC010000061 DAOQOC010000000
VERSION     DAOQOC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7030_length_4985_cov_3.727992"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4508
                     /gene="sprA"
                     /locus_tag="PL106_01655"
     CDS             <1..4508
                     /gene="sprA"
                     /locus_tag="PL106_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNA31801.1"
                     /translation="DPAAWTPEGYFADPERNWAGITRRITTTDFELSNIETIQFWLMD
                     PFSENTSNSNGEPASNEDSQNITGGDFYIDLGNISEDVLRDGRKSFENGLPSNETDQA
                     STTTSTDWGYVPTTQSVVNAFAINDNNSYARQDAGLDGLRDGVNNLDIPSEQDFPENA
                     AYLSAMNGIISANPARRDSLGQDPSGDRYHFFRGGDYDSGGAGQPFGILRRYKWFNGP
                     EGNSISDEDSPEDYPTQQTTLPSTEDINQDQNLAETESYFQYKVSLRPQDMVVGRNFI
                     TDRIEGRSQDGSRTVYWYQFKIPVRQPEKKVGAIQDFRSIRFMRLLVRDWQQQATLRF
                     ARLEFIRGEWRKYTQSLESPGEVPGGDPDATQFNVAAVNIEENGRRTPINYVLPPGIN
                     KETDVSSANLRNLNEQSLQLQVCGLKDGDSRAAFRNVSFDIRSYKKMRMFVHAETRDP
                     ADPLEYGDISVYLRIGNDFDQNYYEYEVPVKPSPFGNNDPYSVWPEANDMVIEFARLN
                     DVKIGRNQANAPVTLRYTVFDGDRRITVKGNPNLSRMTTIMVGIRNPGKDGADANPWV
                     ADDGRTQCAEVWVNELRLTDFDQHGGWAALARGNTTLADLGNLSVAGNYSTPYWGSID
                     KRVSERQRDTRYGVDVSANLEMGKFLPATTGVKVPLYLGYSEQIINPQYDPLTPDIEW
                     TDATRTLTREERKDRLKQLRTYTRRRSINVTNVHKERGSGGGDKGGDKGGGKGSGPPK
                     EHFWDVENLSLSYAYSDQEYHDVITQYENTRTYRGSLAWQHNPKPIAIKPFENIGFIS
                     KSKWTKLIKDFNVNLGFKQLTARTAIDRTYLERLVRANPDLEGLPPVPTYNKTFTWNT
                     QYGFRYEITKNLKVDFSANRNSIVGEPPGRVNTKDRPNYELWKDSVLQSIGKFGLPTQ
                     YDHNVNVTYTLPLDKLPLTDWITVNTSYGTAYKWDRAPLSQDSIGNTIQNSRTISVNG
                     QLNFVNLYNKIKYLKKINDKAKGGGKKPGGKGKTDQKGKDGKGGEEDEKDKGKKRDED
                     RKFDPLAGLFRVLMAVRTGNITYSQNNGTLLPGWGRGPNVIGMDRGFGAPGFGFILGE
                     QNTNLSGDVVRDFATRAAANDWLVKNESIFTPYTSNRSENITARVNLEPFKGMRIDVS
                     ANRTSTDNRSSFFRWREGDVPGTGAYVNDSPRDMGAFSVSMFNWPTTFAKDDENHMNQ
                     LFANLLAYRETVSARLGNQNENSFLPSDLPQDSSFWHGYGATHQDVIIPAFIAAYTGK
                     DPNSVKLNPMKMLPAPNWDITYDGLTKLELFKKVFRTFTVNHSYRSVFSIGGYQTNMY
                     YQPGRDTTDIQGNFIPERQISTITIAEVMRPFINFDATLQNSLLAKFEYNRDRNLSLG
                     LANNQITEVRGKEFVFGTGYRFKDVKFPVIIGGKRPESDLNLRVDLSWRQNNTVIRKI
                     EQKQNTITAGQDIISIKTSADYVIDQRLNVRAFYERVINKPVISTTFPSANTNFGISL
                     RFTLAPQ"
     gene            4572..4949
                     /gene="gcvH"
                     /locus_tag="PL106_01660"
     CDS             4572..4949
                     /gene="gcvH"
                     /locus_tag="PL106_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNA31802.1"
                     /translation="MNIPADLKYTKDHEWIRIEGGEAVVGVTDFAQGELGDIVFVDIG
                     SEGQEVDQHAVFGTIEAVKTVSDLFMPVKGTVAQVNPALADDPAAVNKDPYGAGWMVR
                     ITVSDPAQLAGLLSADQYKALLG"
BASE COUNT         1157 a         1640 c         1471 g          717 t
ORIGIN      
        1 ccgaccccgc ggcctggacc ccggaaggct acttcgccga tccggagcgg aactgggccg
       61 gcatcacacg gcgcatcacc accaccgatt tcgagctcag caacatcgag accatccagt
      121 tctggctgat ggaccccttc agcgagaaca catcgaacag caatggcgaa ccggccagca
      181 acgaggactc gcagaacatc acgggcggcg acttctacat cgacctcggg aacatcagcg
      241 aggacgtgct gcgcgacggc cgtaagagct tcgagaacgg cctgcccagc aacgagaccg
      301 accaggcgag tacgaccacc tccaccgatt ggggctatgt gcccaccacc cagagcgtgg
      361 tgaacgcctt cgcgatcaac gacaacaact cctacgcccg ccaggacgcc gggctggacg
      421 ggcttcgcga cggcgtcaac aacctggata tccccagcga gcaggacttt cccgagaacg
      481 cggcgtacct ctcggccatg aacggcatca tctccgccaa cccggcccgc cgcgattcct
      541 tggggcagga ccccagtggc gacaggtatc atttcttccg gggcggcgat tacgacagcg
      601 gcggtgcggg ccagcccttc ggcatcctgc gccgttacaa gtggttcaac gggcccgagg
      661 gcaactccat cagcgacgag gacagtccgg aggactaccc cacccagcag accaccctgc
      721 ccagcaccga ggacatcaac caggaccaga acctggccga aacggaaagc tacttccagt
      781 acaaggtgag cctgcggccg caggacatgg tggtgggccg caacttcatc accgaccgca
      841 tcgaggggcg cagccaggac gggtcgcgca ccgtctactg gtatcaattc aagatcccgg
      901 tgcgccagcc ggagaagaag gtgggcgcca tccaggactt ccggagcatc cgcttcatgc
      961 gcctgctggt gcgcgactgg cagcagcagg ccacactgcg tttcgcccgc ctggagttca
     1021 tccgcggcga gtggcgcaag tacacccaga gcctggaatc gcccggcgag gtgcccggtg
     1081 gcgatcccga tgcgacacag ttcaacgtgg ccgccgtgaa catcgaggag aacggacggc
     1141 gcacccccat caactacgtg ctgccacccg gcatcaacaa ggagaccgat gtgagcagcg
     1201 cgaacctgcg caacctgaac gaacagtcgc tccagttgca ggtgtgcggc ctgaaggacg
     1261 gcgactcgcg tgccgccttc cgcaacgtga gtttcgacat ccgcagctac aagaagatgc
     1321 gcatgttcgt gcatgccgag acccgggacc cggcggaccc gctggagtac ggcgacatca
     1381 gcgtgtacct gcgcatcggc aacgatttcg accagaacta ttacgagtac gaggtgcccg
     1441 tgaagccctc acccttcggc aacaatgatc cgtacagcgt gtggcccgag gcgaacgaca
     1501 tggtgatcga gttcgcccgg ctcaacgatg tgaagatcgg gcgcaaccag gccaacgcgc
     1561 cggtgacgtt gcggtatacc gttttcgatg gggaccggcg catcacggtg aagggcaacc
     1621 ccaacctcag tcgcatgacc accatcatgg tgggcatccg caacccgggc aaggatggcg
     1681 ccgatgccaa cccctgggtg gccgatgatg gacgcaccca atgcgccgag gtctgggtga
     1741 acgaattgcg cctcaccgac ttcgaccagc acggcggttg ggcggcgctg gcccggggga
     1801 acacgacact ggccgacctg gggaacctga gcgttgcggg caactacagc acgccgtatt
     1861 ggggcagcat cgacaagcgc gtgagcgagc gccagcgcga tacgcgttac ggtgtggacg
     1921 tgagcgccaa cttggagatg ggcaaattcc tgcccgcgac caccggcgtc aaagtgccgc
     1981 tctacctggg gtacagcgag cagatcatca atccgcagta tgacccgctc accccggaca
     2041 tcgaatggac cgatgccacg cgcaccctca cgcgcgagga acgcaaggat cgcctgaagc
     2101 aattgcgcac gtacacgcgg cggcggagca tcaacgtcac caacgtgcac aaggagcgtg
     2161 gatcgggagg tggcgacaag ggcggcgaca agggcggagg caagggcagc ggtcccccga
     2221 aggagcattt ctgggacgtg gagaacctga gcctgagcta cgcgtacagc gatcaggagt
     2281 accacgatgt gatcacccag tacgagaaca cccgcaccta ccgcggctcc cttgcctggc
     2341 agcacaaccc caagcccatc gccatcaagc cgttcgagaa catcggcttc atcagcaaga
     2401 gcaagtggac caagctgatc aaggatttca atgtgaacct cggcttcaaa cagctcaccg
     2461 cacgcacggc catcgaccgg acctacctgg agcgattggt gcgtgccaac cccgacctgg
     2521 agggcctgcc gccggtaccc acctacaaca agaccttcac ctggaacaca cagtacgggt
     2581 tccgctatga gatcaccaag aacctgaagg tggacttcag cgccaaccgc aactcgatcg
     2641 tgggagagcc tccgggccgc gtgaacacca aggaccgccc caattacgag ttgtggaagg
     2701 acagcgtgct gcagagcatc gggaaattcg gcctgcccac gcagtacgac cacaacgtga
     2761 acgtcaccta tacgctccca ttggacaagc tcccgctcac cgattggatc acggtgaaca
     2821 ccagttacgg cacggcctac aagtgggacc gcgcgccact cagccaggac agcatcggca
     2881 acaccatcca gaactcacgg accatcagcg tgaacggcca gctgaacttc gtgaacctgt
     2941 acaacaagat aaaatacctg aagaagatca acgacaaggc caagggcggt ggcaagaaac
     3001 ccggcggcaa gggcaagacc gatcagaaag ggaaggacgg aaaaggcggt gaggaggacg
     3061 agaaggacaa ggggaaaaag cgggacgagg acaggaagtt cgaccccttg gcgggcttgt
     3121 tccgtgtgct gatggccgtg cgcacgggca acatcacgta cagccagaac aacggcaccc
     3181 tgctgcccgg ctggggccgt ggacccaacg tgatcggcat ggaccgcggc ttcggagcac
     3241 cgggcttcgg cttcatcctc ggggagcaga acaccaacct gagcggcgat gtggtacggg
     3301 acttcgcgac gcgcgccgcg gccaacgact ggctggtgaa gaacgagagc atcttcaccc
     3361 cgtacaccag caaccgttcg gagaacatca ccgcgcgcgt caacctggag ccgttcaaag
     3421 gcatgcggat cgacgtgagt gccaaccgga ccagcaccga caaccggtcc tccttcttcc
     3481 gctggcgcga gggcgatgtg cccgggaccg gcgcttacgt gaacgacagc ccgcgcgaca
     3541 tgggcgcatt cagcgtgagc atgttcaact ggcccaccac gttcgccaag gacgacgaga
     3601 accacatgaa ccagctcttc gcgaacctgc tggcctatcg cgaaacggtg agtgcgcgcc
     3661 tcggcaacca gaacgagaac tccttcctgc cgagcgacct gccgcaggac agcagcttct
     3721 ggcacggcta cggggccacg caccaggacg tgatcatccc cgccttcatc gccgcataca
     3781 ccggcaagga ccccaacagc gtgaagttga accccatgaa gatgctcccc gcccccaact
     3841 gggacatcac gtacgacggc ctcaccaagc tggaactctt caagaaggtg ttccgcacct
     3901 tcaccgtgaa ccacagctac cgcagcgtct tcagcatcgg cgggtaccag accaacatgt
     3961 actaccagcc cggtcgcgat accaccgaca tccagggcaa cttcatcccc gagcgccaga
     4021 tctccacgat caccatcgcc gaggtgatgc gccccttcat caactttgat gcgacactcc
     4081 agaacagcct gctggccaag ttcgagtaca accgcgaccg caacctgagc ctcggcctgg
     4141 ccaacaacca gatcaccgaa gttcggggca aggaattcgt tttcggcacg ggctaccgct
     4201 tcaaggacgt gaaattcccc gtgatcatcg gcgggaaacg gcccgagagc gacctgaacc
     4261 tgcgcgtgga cctcagctgg cgccagaaca acaccgtcat ccggaagatc gagcagaagc
     4321 agaacaccat caccgcgggc caggacatca tctccatcaa gaccagcgcc gactacgtga
     4381 tcgaccagcg gctcaacgtg cgcgccttct acgagcgcgt catcaacaag cccgtcatca
     4441 gcaccacctt ccccagtgcc aacaccaact tcggcatcag cctgcgcttc acactggcac
     4501 cgcaataagt gacgtgccgg tcggggcctc agctaatttc gccacccacg gacaacaaag
     4561 ccaccatcgc catgaacatc ccggcagacc tcaaatatac caaagaccac gaatggatcc
     4621 gcatcgaagg cggcgaggcc gtcgtgggag tcaccgattt cgcgcagggc gagctgggcg
     4681 atatcgtgtt cgtggacatc ggttctgaag gacaggaagt ggaccagcac gcggttttcg
     4741 gcaccatcga ggccgtgaag accgtgagtg acctgttcat gccggtgaag ggcaccgtcg
     4801 cacaagtgaa cccggcgctc gccgatgatc ccgccgccgt gaacaaggac ccgtacggtg
     4861 cgggatggat ggtgcggatc accgtcagtg atccggccca attggccggg ctgctgagcg
     4921 ccgaccagta caaggcgttg ctgggctgaa ccaggaatgc tgcgccacct gcgctgggcc
     4981 ctcct
//
LOCUS       DAOQOC010000062         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7099_length_4961_cov_3.902976, whole genome shotgun sequence.
ACCESSION   DAOQOC010000062 DAOQOC010000000
VERSION     DAOQOC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_4961_cov_3.902976"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PL106_01665"
     CDS             <1..258
                     /locus_tag="PL106_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31803.1"
                     /translation="TITTDSMRMHILKNEAFDYVLLCNKICGASHYNMQMPLVVEPTS
                     DYNAWLAQQKGFEMASTPAAPVEATTTTAVLEAATTAPNTH"
     gene            261..2066
                     /locus_tag="PL106_01670"
     CDS             261..2066
                     /locus_tag="PL106_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNA31804.1"
                     /translation="MGHTAHTTATAQGHAHAAHHHHEESFITKYIFSQDHKMISRQFL
                     ITAIIMAWVAMIMSTLFRLQLAWPGEGFAITNFLLGDKWAPEGVLSPDKYLALVTIHG
                     TIMVFFVLTGGLSGTFANLLIPFQVGARDMASGFLNMLSYWFFFTSSVVMIASLFVEG
                     GPASVGWTVYPPLSALPKAIPGAGTGMTLWLVSMVLFIASTLLGGLNYIVTILNLRTK
                     GMRMTRLPLTVWALLITAVVGVLSFPVLVSAAVLLLMDRLAGTSFYLSEIHIAGEALD
                     NMGGSPILFQHLFWFLGHPEVYIVLLPALGITSEVIATNARKPIFGYRAMIGSLCAIG
                     FLSCIVWGHHMFITGMNPFLGSVFVFTTLLIAIPSAVKVFNYLTTLWRGNLVLSPAML
                     FSIGLVTSFIAGGLTGIILADSALDIMVHDTYFVVAHFHVVMGLSAIFGMFAGVYHWF
                     PRMFGRMMNNRLGYAHFWITFICAFGVFLPMHYVGLAGAPRRYYDSSNFPFLDGVMDL
                     NVLITVFAIVGALAQIIFTYNFFNSIFHGQKSVQNPWKSNTLEWTAPITPMHGNWPGP
                     LPEVHRWPYDYSKPGKEEDFVPQTVPLDPGEEDGH"
     gene            2109..2795
                     /locus_tag="PL106_01675"
     CDS             2109..2795
                     /locus_tag="PL106_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31805.1"
                     /translation="MKDEWRVERPALRTTRHSFIPTHLFLAIFCLGVWSGSTAQRIDS
                     IPHYLHERRSPVVKLDMRGSFIGNQQVNFAGIKLGLSHAGVVQYGIGYSFLLSPVRGT
                     AWIPGLGERTTRLRLGYVAPYFDFAFYKKGHWELRLPVQIGIGGGSVVYDGADGRTQK
                     LEQSMVFIYEPCMTAQYRFLRYLAVAGGWGYRLTLRSAHLADGLTAPIYIFELRVFFG
                     DIWRDVKHGS"
     gene            3053..4078
                     /gene="ruvB"
                     /locus_tag="PL106_01680"
     CDS             3053..4078
                     /gene="ruvB"
                     /locus_tag="PL106_01680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNA31806.1"
                     /translation="MADTFDVRNEQRSAADKEMEQALRPRRFGEFAGQQAVTDNLKVF
                     VQAAKLRGEALDHVLLHGPPGLGKTTLAGIIAQEMGVSMRVTSGPVLDKPADLAGLLT
                     NLQPNDLLFIDEIHRLSPVVEEYLYSAMEDYRIDLVIDAGPNARTVQITLNPFTLVGA
                     TTRSGLLTAPLRARFGINSRLDYYDAGTLKRILHRSSGLMNVPIVDEAADEIARRSRG
                     TPRIANALLRRVRDFAQIQGDGTIDLGIAQHALKALNVDAHGLDEMDNRILACIIEKF
                     AGGPVGLNTVATAVGEESGTIEEVYEPFLIMEGYLQRTPRGRLCTERAYRHLGKAPQA
                     RPGDLFN"
     gene            complement(4088..>4961)
                     /locus_tag="PL106_01685"
     CDS             complement(4088..>4961)
                     /locus_tag="PL106_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31807.1"
                     /translation="PFQAPGRAGNGMKWDLSGLPAGSVVPYYWTSINTAPGAGAFPDD
                     AAVLQVPGEPAAYYQLGDTALYWLGTYADTALVRFDPPMAIVDLPCSINSTWSDSGVA
                     AVTGAGRIDIRVTAYHAQADAYGTLVLPYGTVDKVLRVRSELTATDKFPPHRLRLHEV
                     RYAWYCDRTPMPLLIVVERTGWPPPQRYMRWLDGSWQDDPGRLFKPIVLHPFPDPCDD
                     IITVDLPAARADRTVLQLLDEQGNARKQWPVEFNSPQTRRMTLEMSEVPSGNYTLNWI
                     GTNGTIGNARLEKR"
BASE COUNT          928 a         1549 c         1516 g          968 t
ORIGIN      
        1 acgatcacca cggacagtat gcgcatgcac atcctgaaga acgaggcctt cgactacgtg
       61 ctgctgtgca acaagatctg cggcgcctcg cactacaaca tgcagatgcc gctggtagtg
      121 gagcccacca gcgactacaa cgcgtggctg gcgcaacaga agggcttcga gatggcctcg
      181 acgccagccg cacccgttga ggcaacgacc accacggccg ttttggaagc cgccactacc
      241 gctccgaaca cgcattgaac atgggccaca ccgctcacac cacggctact gcgcaaggcc
      301 atgcgcacgc ggcccatcac caccacgagg agagcttcat tacgaagtac atcttctcgc
      361 aggaccacaa gatgatcagc aggcagttcc tgatcacggc gatcatcatg gcttgggtgg
      421 ccatgatcat gtcgaccttg ttccgcctgc aactggcatg gcccggtgaa ggattcgcga
      481 tcaccaactt cctgctgggc gacaagtggg cccctgaagg ggtgttgagc ccggacaagt
      541 acctggcact ggttactata cacggcacca tcatggtgtt cttcgtactc accggcggat
      601 tgagcggaac cttcgcgaat ctcttgatcc ctttccaggt cggtgcgcgc gacatggcga
      661 gcggtttcct caacatgctc agttattggt tcttcttcac gagcagcgtg gtgatgatcg
      721 cctcgctctt cgtggagggc ggtccggcct ccgtcggctg gacggtgtac ccgccactga
      781 gcgcactgcc caaggccatt cccggcgcgg gcacgggcat gaccctctgg ttggtgagca
      841 tggtgctctt catcgccagt acgctgctcg gtggcctgaa ctacatcgtc accatcctca
      901 acctgcgcac caagggcatg cgcatgaccc ggcttcccct gacggtctgg gcattgctga
      961 tcacggccgt ggtgggcgtg ctcagtttcc ctgtgctggt gagcgctgct gtattgctgc
     1021 tgatggatcg cttggccgga acgagcttct acctcagcga gatccacatc gccggtgagg
     1081 cgttggacaa catgggcggc tcaccgatcc tcttccagca cctgttctgg ttcctgggtc
     1141 accccgaggt gtacattgta ctgctcccgg ccttgggcat cacttcggag gtgatcgcga
     1201 ccaatgcccg caagcccatc ttcggctatc gtgccatgat cggatcgcta tgcgcgatcg
     1261 gcttcctcag ctgcatcgtg tggggccacc acatgttcat cacgggcatg aaccccttcc
     1321 tgggcagcgt gttcgtgttc accaccctcc tgatcgccat ccccagcgcg gtgaaggtgt
     1381 tcaattacct caccacgctc tggcggggca acctggtgct cagccccgcc atgctcttca
     1441 gtatcggcct ggtgacgtcc ttcattgcgg gcggcctcac gggcatcatc ctggctgaca
     1501 gcgcactgga catcatggtg catgacacct atttcgttgt ggcgcacttc cacgtggtaa
     1561 tgggcttgag cgccatcttc ggcatgttcg ccggggttta ccactggttc ccgcgcatgt
     1621 tcggacgtat gatgaacaat cgcctcgggt acgcgcactt ctggatcacc ttcatctgcg
     1681 ccttcggggt cttccttccc atgcactacg tgggtctggc cggtgcgccg cgccgctact
     1741 acgattccag caacttcccc ttcctggacg gggtgatgga cctcaacgtc ctcatcaccg
     1801 tcttcgccat cgtgggcgct ctggcccaga tcatattcac gtacaacttc ttcaacagca
     1861 tcttccacgg acagaagagc gttcagaacc cctggaagag caataccttg gaatggaccg
     1921 caccgatcac gccgatgcac ggcaactggc ccggtccgct tccagaggtg catcgctggc
     1981 cgtatgacta cagcaagccg ggcaaggagg aggatttcgt gccgcaaacc gtgccgctgg
     2041 acccgggcga ggaggacggc cattgagccg gtgttcgtag aggaccaccg atgatccgct
     2101 aacaagcgat gaaagacgag tggcgagtgg agcgcccggc actgcgcacc actcgccact
     2161 cgttcattcc gacccacctc ttcctcgcga tcttctgtct gggcgtctgg tccggctcaa
     2221 ccgctcaacg gatcgatagc attcctcact acctgcacga gcgtagaagt ccggtggtga
     2281 agctggacat gcgcgggtcc ttcatcggca atcagcaggt gaacttcgcc gggatcaaat
     2341 tggggctgag tcatgcggga gtggtgcagt acggcatcgg gtacagcttt ctattatctc
     2401 cagttcgtgg cacagcgtgg atcccgggcc tcggtgagcg caccacacgc ctccgattgg
     2461 gctacgtggc cccctacttc gacttcgcct tctacaagaa gggccattgg gagttgcgcc
     2521 tgcccgtgca gatcggcatc ggcggaggca gcgtggtgta cgatggtgcg gatggccgaa
     2581 cacagaaact cgagcagagc atggtgttca tctatgaacc ctgcatgacc gcgcaatacc
     2641 gcttcctgcg ttacctggct gtagcgggcg gctggggcta ccgcctcacc ttgcggagcg
     2701 cacacctggc cgatggtctc acagcaccca tctacatctt cgaactgcgg gtatttttcg
     2761 gggatatctg gagggatgtg aagcatggat cgtgaacagg tcggtcgcca ttcggtgtgc
     2821 tgtacactgt tcatccaata ccctttcgca attcagcctc atccgatcga tccatgaaat
     2881 gccgtgctga atccgtcgac ggttcggcaa gctcaccgct tcgacaggct cagcgtgaca
     2941 tattggatgg ctccatactg cgaaatggta taacactgag gcggcgccgg catcagaccc
     3001 cgcaccgcta cttttggacc tcgccttcgt cattgacttc ggcgagtaaa ggatggctga
     3061 caccttcgac gtacgcaacg agcagcgctc cgcggctgac aaggagatgg agcaggcgct
     3121 gcgcccgcgc cgtttcgggg agttcgcggg gcagcaggcc gttacggaca acctgaaggt
     3181 cttcgtgcag gccgccaagc tgcgtgggga ggcgttggac cacgtgctgc tgcacggtcc
     3241 accaggcctg ggcaagacca cattggcggg catcatcgcc caggaaatgg gggtgagcat
     3301 gcgcgtgacc agtggcccgg tgctggacaa accggcggat ctggccggcc tgctcaccaa
     3361 cctgcagccg aacgacttgc tcttcattga cgagatccac cggctgagcc ccgtggtgga
     3421 ggaatacctg tacagcgcca tggaggacta ccgtatcgac ctggtgatcg acgcgggccc
     3481 gaacgcacgt acggtgcaga tcacactgaa cccgttcacg ctggtgggtg ccaccacccg
     3541 cagtggcctg ctaaccgcac cattgcgcgc gcgcttcggc atcaacagcc ggttggacta
     3601 ctacgatgca ggtacattga aacgcatcct tcaccgctcc tcgggcctca tgaacgtgcc
     3661 gatcgtggat gaggcggcgg acgagatcgc acggcgcagc cggggcacac cgcgcattgc
     3721 gaacgccttg ttgcggcgcg tgcgcgactt cgcgcagatc cagggcgacg gcaccatcga
     3781 tctgggcatc gcgcagcacg cgttgaaagc cctgaacgtg gacgcgcacg ggctggacga
     3841 aatggacaac cgcatcctgg cgtgcatcat cgagaagttc gctggtggac cggtgggact
     3901 gaacacggtg gccacggccg tgggcgagga gagcggcacc atcgaggagg tgtatgagcc
     3961 cttcctgatc atggaaggct acctgcaacg caccccgcgt ggaaggctgt gtacggagcg
     4021 ggcctaccgc catttgggca aggcgccaca ggcccggccc ggggacctgt tcaattgagt
     4081 acgacagcta ccgcttctcc aggcgggcgt tcccgatggt gccgttggtg ccgatccagt
     4141 tgagcgtgta gttgccggat gggacctcgg acatctccaa cgtcatccgt cgcgtttgcg
     4201 ggctgttgaa ctccacgggc cactgcttgc gcgcattacc ctgctcatcg agcaattgca
     4261 gcacggtgcg gtcggccctc gcggccggca ggtccacggt gatgatgtca tcacaaggat
     4321 ccgggaaggg gtgcagcacg atgggcttga agagtctgcc ggggtcgtcc tgccaggaac
     4381 catcgagcca gcgcatatag cgctgcggtg gcggccagcc ggtacgctcc accacgatga
     4441 gcaggggcat gggtgtacgg tcgcagtacc aggcgtagcg cacctcatgc aggcgcaggc
     4501 gatggggcgg gaacttgtcg gtcgcggtga gttcggaccg cacacgcaag accttgtcca
     4561 ccgttccgta cggcagcacg agcgttccat acgcatcggc ctgggcgtgg taggcggtca
     4621 cccggatgtc gatacggccc gcgccggtga ccgcggccac cccggaatcg ctccacgttg
     4681 aattgatgct gcacggaagg tcaacaatgg ccatgggcgg atcgaagcga acgagggcgg
     4741 tatcggcata ggtgccgagc cagtacaggg cggtgtcgcc cagctgatag tacgccgcgg
     4801 gttctccagg cacttgcaag acggcggcat catcggggaa ggctccggcc ccgggagccg
     4861 tgttgatact tgtccaatag tacggcacca ccgatcccgc cggcaaaccg ctgagatccc
     4921 atttcatccc gttaccggcc cggcccgggg cctggaaggg c
//
LOCUS       DAOQOC010000063         4913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7223_length_4913_cov_4.143269, whole genome shotgun sequence.
ACCESSION   DAOQOC010000063 DAOQOC010000000
VERSION     DAOQOC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7223_length_4913_cov_4.143269"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PL106_01690"
     CDS             complement(<1..1241)
                     /locus_tag="PL106_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31808.1"
                     /translation="MHRNASLLLLALAAGLLAWVVKDAPPDERLADASLRLERQIHAA
                     QDQLHADLDGVRRATVEGTGCTTEEAWPTIDPALRAQWETQGTAFRVLCDNALVAWSG
                     SVPLEQGPEGSADFIRRQNGCWLYARAGVNEHVQVDAIREVWYVPPFENRYLRGRFNP
                     AYDVEEGIEASLAAGLGPVVREAGGAVLFRLRWKEDVPPPGAGAHWRAILVLLCVILV
                     AAVVWRLLSGIATNGRPWQALIGMLVLCGLSCFLLLGNGPLSLLSGFRWADPDLFASA
                     HLPSFAHLLLATALVTLAAAFSHRMLRSVAPPLWPVPAAALVLALLLGSAALINEVMI
                     ALVRDSRIPLDLFHAQELNLDSMAALLAIACLLFAWTLLADGPTRWILPGIGRRTALI
                     IVLGAFVAAHLAYHYTGMYDSV"
     gene            1428..3167
                     /locus_tag="PL106_01695"
     CDS             1428..3167
                     /locus_tag="PL106_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31809.1"
                     /translation="MRHANLFTAILAPVLALAQPQFVNPGFENWQNLGTGTEEPTDWS
                     SIKTSDASALINNLAPQVCWRSTDFHSGAYSVRLKSVNTTIGVVANGTLTCGRVHAEF
                     TPSNGYVFTDAGNADWNQALTSKPDSLIGWFKATPQPGDKGKVEAILHTGAGQLPEGS
                     TFPNWNARARWIAPSATVGSWTRFSVPFHYYNAGTPSHILLVLTSGDSLVTQENTEAW
                     YDDIGLVYNIIPSPSVSSVALNGIDPAAITVGYSTGGIPVASTLFTAQLSNASGGFGS
                     PVTIGTLTGTSASGTINCSIPGNTAPGAGYRIRVIASNSAYAPVPVPFSITGTSTVKL
                     AAKVRLEGAYVPSTGSMGDALRTLPAFPLTEPYSALGYAHVAGGGETTTAPVLAVTGN
                     NAIVDWVVLELRSTSSPYAVLATRSALLQRDGDVVDVDGVSPVSLSAGAGNYRVAVRH
                     RNHLGCMTASAVSLSSTPATLDFSAAATSTYGTAARKSIAGAFPTQALWAGDVTFNGQ
                     VKYTGSGNDRDPILITVGSSAPNNVVTGTYSGRDVNLNGEVKYTGSGNDRDPILVNVG
                     GTAPNNVRSQQLP"
     gene            3211..4635
                     /locus_tag="PL106_01700"
     CDS             3211..4635
                     /locus_tag="PL106_01700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA31810.1"
                     /translation="MPLSITFHGAAGMVTGSKHLLEFPGRDGKTVRLLLDCGLFQGEG
                     PKSDEKNRRFGFDPASINVLVLSHAHIDHSGLIPRLVREGFKGPIWATPATRDLCAIM
                     LEDSARIQESDFEQDQKRARRHGRVPQEKEPLYTVDDVPPAMALFQGVDLNEPFAILP
                     EVKLLFTDAGHILGSACVHLDIDTGEGVRRISFSGDVGRYVDRLLPDPVRFRQAGIII
                     CESTYGDRDHPPANESRDQLLAHILRVCVERRGKLIIPAFSIGRTQEILHTLNGLHNE
                     GRLPRIPVFVDSPLSINATAIYRRYAHLLQEDVRAELAADPDLFGFRGVEFVREAARS
                     KQLNDRQEPCIVIAASGMMEAGRVRHHLLHGLPNERNAVLVVGFCAPGSLGAEILERP
                     DHVHIFGERVPVRAEVLRMEGYSAHADRNELMRWISCQNAALVERVFLVHGVPASASG
                     LRERLTAQGYQRIDIPGQGQRFEV"
     gene            complement(4665..>4913)
                     /locus_tag="PL106_01705"
     CDS             complement(4665..>4913)
                     /locus_tag="PL106_01705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA31811.1"
                     /translation="LKGWKQYRLRGMACHPDLRGHGAGTLVLRFGIGHLKDQRADLLW
                     CNARENAVGFYKKLGFSTHGSPFLIEGIGMHELLFLRL"
BASE COUNT          971 a         1617 c         1532 g          793 t
ORIGIN      
        1 acggaatcat acatgccggt atagtggtac gccaggtgtg cggcgacgaa ggcacccagg
       61 acgatgatga gcgccgttcg tcggccgatg ccgggcagga tccatcgcgt aggtccgtcg
      121 gccagcaacg tccaggcgaa aagcaagcat gcgatggcca aaagtgcggc catgctgtcc
      181 aggttcagct cctgtgcatg gaagaggtcg agcggtatgc ggctgtcgcg cacgagagcg
      241 atcatcacct cgttgatcaa cgccgccgac cccagcagca aggcgagtac gagcgcggcc
      301 gcgggtacgg gccagagcgg tggtgccaca ctgcgcagca tgcggtggga aaaagcggca
      361 gccaacgtga ccagtgcggt cgcgagaagc aaatgggcga acgagggaag gtgtgccgag
      421 gcgaacaggt ccggatcggc ccagcggaac ccggacagca gggacaatgg cccgttgccc
      481 agcaacagga agcaggacag gccacagagg accagcatcc cgatcaacgc ctgccatggc
      541 ctgccattgg tcgcgatccc cgacaggagt ctccagacga ccgcggcaac aaggatcacg
      601 cacagcagca caaggatcgc tcgccagtgc gcaccggcgc cgggaggcgg cacatcctcc
      661 ttccagcgca aacggaacaa caccgcacca ccggcctcac gcaccaccgg cccgagtccc
      721 gcggccaaag aggcttcaat gccttcttcc acatcataag ccggattgaa acgaccgcgc
      781 aggtagcgat tctcgaacgg tggtacgtac cacacctcgc gtatggcatc cacctgcaca
      841 tgctcattca cgccggcgcg cgcgtagagc cagcaaccgt tctgccgccg gatgaagtcc
      901 gcggatccct ccggtccttg ctccagaggc acagaacccg accaggccac cagcgcgttg
      961 tcgcagagaa cgcggaaagc ggtgccctgt gtctcccatt gagcacgcaa ggccggatcg
     1021 atggtgggcc aggcctcctc ggtggtgcag cccgtgcctt ccaccgtcgc cctgcgcaca
     1081 ccgtccaggt cagcgtgcag ttgatcctgc gccgcgtgga tctgtcgctc caggcgaagc
     1141 gaagcatcgg caaggcgctc atcgggcggt gcatccttca ccacccaagc gagcaggccc
     1201 gctgcaagtg cgagcaaaag gagcgatgcg ttgcgatgca tgggagatgc gctcaaagaa
     1261 acccggggat cggtcactgg accatggcca tgcacatgag ttaccaaagt cggaccgccg
     1321 ataaccggtg cggcatcgga ccggtcatca cctctccggg atgccgggga ggaacggttt
     1381 ggcattccgg gaacacaccg gtacgtttgg accccattca gaagcgcatg agacacgcga
     1441 atcttttcac cgcgatcctc gcccccgtcc tggctctggc ccagccccaa ttcgtcaatc
     1501 ccggttttga gaactggcag aacctcggta ccggaacgga agagcccacg gactggagct
     1561 cgatcaagac cagcgacgcg agtgccctga tcaacaacct cgccccgcag gtgtgctggc
     1621 gcagcaccga cttccattcg ggtgcgtatt ccgtccggct caagtcggtg aacaccacca
     1681 tcggcgtggt ggccaacggc acgctcacat gcggtcgtgt gcatgcggaa ttcactcctt
     1741 ctaacgggta tgttttcacc gatgccggca atgcggactg gaaccaggcg ctcaccagca
     1801 aacccgacag tctcatcgga tggttcaagg ccacaccgca gcccggcgac aagggcaagg
     1861 tggaggctat cctgcacacc ggggcgggcc aactgccgga ggggtccacc ttcccgaatt
     1921 ggaacgctcg cgcccgttgg atcgcaccct cggccaccgt gggttcgtgg acacggttct
     1981 cggtcccctt ccactactac aatgcgggta cgccaagcca tatcctcctg gtgctcacca
     2041 gcggcgacag cctggtcacc caggagaaca ccgaagcctg gtacgacgac atcggtctcg
     2101 tgtacaacat cattccctca cccagcgtct cctcggttgc gctcaatggc atcgacccgg
     2161 cggccatcac ggtggggtac agcaccggcg ggatccccgt ggccagcacg ctcttcaccg
     2221 cgcaactgag caatgccagc ggtggcttcg gttcaccggt caccatcggt acgctcacgg
     2281 gcacatccgc atcgggaacg atcaactgca gcatcccggg caacacggcc cccggggcag
     2341 gataccggat ccgcgtgatc gcatccaata gcgcgtacgc accggtaccc gtgcccttca
     2401 gcatcaccgg tacatccacg gtgaagctcg cggccaaggt gcggctggag ggcgcgtacg
     2461 tgccgtcgac cggatcgatg ggcgatgcac tgcgcacact gccggctttc cccctcaccg
     2521 agccgtacag cgcgttgggc tatgcccacg ttgccggagg tggggagacc acaacggcgc
     2581 ccgtactggc cgttacgggc aacaacgcca tcgtcgattg ggtggtgctg gaattgcgtt
     2641 caacctcctc cccctacgct gtgctcgcca cgcggagcgc gctgttgcag cgcgatgggg
     2701 acgtggtgga tgtggacggc gtttctccgg tgtccttgag cgccggggcg ggcaactacc
     2761 gagtggccgt gcggcaccgc aaccacctcg gttgcatgac ggccagcgcg gtatcattat
     2821 cgtccacgcc cgcaacgctc gacttctccg ccgccgccac ctccacatac ggtaccgcgg
     2881 cgcgaaagag catcgcaggt gccttcccga cccaggccct ctgggcgggt gatgtcacct
     2941 tcaacggcca ggtcaaatac accggcagtg gcaatgatcg ggatcccatc ctcatcaccg
     3001 tgggcagctc cgcaccgaac aacgtggtga ccggtaccta cagtggccgt gatgtgaacc
     3061 tgaacggcga ggtgaaatat accggcagcg gcaacgaccg ggacccgatc ctggtgaatg
     3121 tgggaggaac ggcgcccaac aacgtgcgct cgcaacaact gccctgatca gaggaacagc
     3181 atccgctccg gaggatccgt tcaaccgcct atgccgcttt cgatcacctt ccatggcgcc
     3241 gccggcatgg tcacggggag caagcacctc ctcgagttcc ccggtcgtga cggcaaaacc
     3301 gtgcggctgt tgctggattg cggcctgttc cagggcgagg gcccaaaaag cgatgagaag
     3361 aaccgccgct tcggattcga cccggcgagc attaatgtgc tggtgctctc gcacgcccac
     3421 atcgaccaca gtggcctgat cccgcgcctg gtgcgcgagg ggttcaaagg accgatctgg
     3481 gccaccccgg ccacacgcga cctctgcgcg atcatgctgg aggacagcgc ccggatacag
     3541 gagagcgatt tcgaacagga ccagaagcgc gcacgtcggc atggccgtgt tccgcaggag
     3601 aaggaaccgc tctacacggt ggatgacgta cctcccgcca tggcgctgtt ccaaggcgtg
     3661 gacctcaacg agcccttcgc catcctcccg gaggtgaagc tcctgttcac cgatgcgggc
     3721 cacatactcg gaagtgcgtg cgtccacctg gacatcgata ccggggaagg ggtgaggcgg
     3781 atctccttca gtggcgacgt gggccggtac gtggaccgtc tgttgcccga tcccgtgcga
     3841 ttccgccagg ccgggatcat catctgcgag agcacctatg gtgaccgcga ccacccgccc
     3901 gccaacgagt cacgggacca attgctggca cacatcctcc gggtatgtgt cgaacgcagg
     3961 ggcaagctga tcatcccggc cttcagcata ggcaggacac aggagatcct gcacacgctg
     4021 aacgggctcc acaacgaggg ccgtctgccc cgaatacctg ttttcgtgga tagcccgctc
     4081 tctatcaacg ccaccgcgat ctatcggcgc tacgcccacc tgctccagga ggatgtgcgc
     4141 gcggaactgg ccgctgaccc ggacctgttc ggcttccggg gcgtggagtt cgttcgcgag
     4201 gccgcgcgga gcaagcagct gaacgacagg caggagccct gcatcgtgat cgccgcgagc
     4261 ggcatgatgg aggccggtcg cgtgcggcac catctgttgc acggactgcc caacgagcgc
     4321 aatgcggtgc tcgtggtggg cttctgcgca ccgggatcat tgggtgccga gatcctggag
     4381 cgtccggatc atgtgcacat cttcggggag cgggttcccg tacgggccga ggtgctgcgc
     4441 atggagggat acagcgcgca cgccgaccgc aacgagctca tgcgctggat ctcgtgccag
     4501 aatgcggctc tcgtggaacg tgtcttcctc gtgcatggcg tccccgcctc ggccagcggc
     4561 ctgcgcgagc ggctcacggc acaagggtac cagcgcattg atatacccgg gcagggacaa
     4621 cggttcgaag tgtgatcgca tcccgtgcgt ccggatgcgc accctcaaag ccggaggaag
     4681 agcaactcgt gcatgccgat gccttcgatg agaaaggggc tgccatgggt gctgaacccg
     4741 agtttcttgt agaagccgac ggcattctca cgcgcattgc accagagcag atcggcgcgc
     4801 tgatccttca ggtggccgat gccgaaacgc aggaccaggg tccccgcgcc atgacccctg
     4861 aggtccggat ggcaggccat gccacgcagc cggtattgtt tccagccctt cag
//
LOCUS       DAOQOC010000064         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7308_length_4883_cov_3.471417, whole genome shotgun sequence.
ACCESSION   DAOQOC010000064 DAOQOC010000000
VERSION     DAOQOC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_4883_cov_3.471417"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2346)
                     /locus_tag="PL106_01710"
     CDS             complement(<1..2346)
                     /locus_tag="PL106_01710"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31812.1"
                     /translation="MRGTTFRTWLPFTIGVLTAALAWWALRPRALPGGLEVTLEVTTT
                     CPDKFELFWDNGSSGYEPDRMLNRTVMPAYDLQRIRFQLPDSVHRVRGLRIDPGTQAV
                     QMTLKAIELRGPFRTKRLEGRDITDRFGAANDLRTLEVDTMRNIVPIKCTGTDPYLAS
                     TIDLGALSSEVMDSSPPYSAPLLLALLIGALFAWAARIITLPQDTGHRPAPPGEPRSM
                     AVRVTAGLIATMAGLVVTGIADNINFHDRALMVDMEVEAARADNFQIFYADRPGGFSE
                     AYYVNCPVQAARGAQLLSFRMPADTLFKFVRFDPGNAQDSLSIRSMSLRCNDRVRRFD
                     AADLFDLLKPNGQVEAFEKRDSTLFLRFNGNDPYLFSDTDFGEDVRALWEDSGNGPLP
                     FCTGLICAIAVFAGLVRSRRLATLIISARPVEWATGIGFCALISLPMLSDVLPIQPSL
                     ADTEKRPLAEKPLLRMHSLEAFPDKYTRYYADHFGYRKLLFRWNSIFHTYALHCSSMP
                     DNVVFGKDGYLFLVKPGVGRYFRNQPVLTEADMKRIAERLDHRKKWLAGLGIDYYLVV
                     PPLKASMYPEKLPDKYRKVDDRSVLDDLKDYLDEHSSVELIDLRPDLAEAKKIRDIYY
                     TTDIHWNPWGGLIGYRTLMHAMLKDHPELGLPCLPEDYAVEADTNDQGDLALQIALND
                     KLTRVTYMMVPKIPFRAKETGDEPLPGDAFFKYKPVFAKGPDPKAPKLLMFRDSFAVY
                     LMPYLNEHFSRSVYVWTPILLPEIVEMEKPDIVVQEIMELFL"
     gene            complement(2350..3774)
                     /locus_tag="PL106_01715"
     CDS             complement(2350..3774)
                     /locus_tag="PL106_01715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA31813.1"
                     /translation="MVFSSPVFLFQFLPLTLLLVLLAGDRKRQNTLLLIASLFFYAWG
                     EKSWVLLMVGTALFNHLCGLAIERWRSPRAVLALAVFANLAVLIWFKYANLFATTINA
                     LLGSDILLLDPVHLPIGVSFFIFHSLSYTVDVFRGDAKAQRSPGITLLYIALFPQLVA
                     GPIIRYHDVADQFTDRRMDVSGFAYGVRRFIIGLAKKVLIADQCARIADPIFHEVPDD
                     VTMPVAWLGITAYAVQIYFDFSGYSDMAIGSARMFGFRFLENFNRPYIATSIREFWKR
                     WHISLTNFFRDYVYIPLGGNRGGPVRTYLNLLIIFTLTGLWHGASWNFVVWGLIHGAF
                     MMAERAGLGRVLDRLPRFVGHVYTLAVVLTAWAFFRIEDITGAWRFANAMWNPSAGDG
                     LLTYPELYLSNVTLLALVLGVAGSVNAHVAIGRMLRLPAFSGEVPPQGVWSAVQLFGL
                     LVLFIGVAMQVASSTFSPFIYFRF"
     gene            complement(3881..4282)
                     /locus_tag="PL106_01720"
     CDS             complement(3881..4282)
                     /locus_tag="PL106_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31814.1"
                     /translation="MQRLIRSFNTLTDDLREMLQEQYPDGIDNSHIRTLNTPKGESLR
                     VIELRTSDAIYLIKINPESREEIEQFLDQEEGGGNMAEDMDTRDGEDIETPGGEDADE
                     DDDDTGDDDDVDGDDDDDDAGDDEKDDDDDA"
     gene            complement(4324..>4883)
                     /locus_tag="PL106_01725"
     CDS             complement(4324..>4883)
                     /locus_tag="PL106_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNA31815.1"
                     /translation="KSGNRRFPHQYFYEMIRSGVLEQYYQIDPKDSWMVAAAERDLPI
                     ICPGWEDSTLGNIFAGHCIVGDCKPQMMKSGIEYMMFLADWYTRNSGTDGIGFFQIGG
                     GIAGDFPICVVPMLHQDLQRDSIPFWSYFCQISDSTTSFGSYSGAVPNEKITWGKLDI
                     DTPKFIIESDATIVAPLIFAYILDL"
BASE COUNT          935 a         1368 c         1632 g          948 t
ORIGIN      
        1 aaggaagagt tccatgatct cctgcaccac gatgtcgggt ttctccatct ccacgatctc
       61 ggggagcagg atgggtgtcc acacatacac gctccggctg aagtgttcgt tgaggtacgg
      121 catcaggtag acggcgaagc tgtcgcggaa catcagcagt ttcggtgcct ttggatccgg
      181 gcccttggcg aacaccggtt tgtacttgaa gaatgcgtca ccgggcaggg gttcgtcccc
      241 cgtttccttt gcgcggaagg gtatcttggg gaccatcata taagtgacgc gcgtgagttt
      301 gtcgttgagc gcgatctgca gggccaggtc cccttggtcg ttggtatcgg cctccacggc
      361 gtagtcctcg ggcaggcacg gcagaccgag ttcggggtga tccttgagca tggcatgcat
      421 cagggtgcga tagccgatga ggccgcccca ggggttccag tgtatgtcgg tggtatagta
      481 gatatcacgg atctttttgg cctcagcgag gtcggggcgc agatcgatga gttcgaccga
      541 actgtgttcg tccagatagt ccttcaggtc atccagtacg gaccggtcgt ccactttccg
      601 gtatttgtcg ggcagcttct cggggtacat ggaggccttc agtggaggga caacgaggta
      661 atagtcaata ccgagcccgg caagccattt cttccggtgg tccagccgct ccgcgatccg
      721 cttcatgtcg gcttcggtga gaacgggctg gttccggaag tagcggccca cacccggttt
      781 gaccagaaaa aggtacccat ccttgccgaa caccacgttg tcgggcatgc tggagcaatg
      841 gagggcatag gtgtggaaga tggagttcca gcggaagagg agcttccggt agccgaagtg
      901 gtccgcgtag tagcgtgtgt acttgtcggg gaatgcctcc agcgagtgca tgcgcaggag
      961 cggcttctcc gcgagaggac gcttctcggt gtcggccagt gaaggttgaa tgggcagcac
     1021 atcgctgagc atgggcaggc tgatcaaggc acagaagccg atgcccgtgg cccattcgac
     1081 cggtcgtgcg gaaatgatga gcgtggccag gcgtcggctg cgcacgagcc cggcgaacac
     1141 ggcgatggcg catatcagtc ctgtgcagaa gggcaatggg ccgttgccgc tatcttccca
     1201 aagcgcgcgc acatcctctc cgaagtcggt atcgctgaac aggtaggggt cattgccgtt
     1261 gaagcgcagg aagagggtgc tgtcgcgctt ttcgaatgcc tcgacctgtc cgttcggttt
     1321 gaggaggtcg aacaggtccg cagcatcgaa tcgccggacc ctgtcgttgc aacgcaggct
     1381 catggagcgt atgctcaggc tgtcctgcgc attgcccggg tcgaaacgca cgaacttgaa
     1441 cagggtatcg gccggcattc ggaagctgag caactgggct cctcgagcgg cctggacagg
     1501 gcagttcacg tagtaggcct cgctgaagcc gcctggccgg tcggcataaa agatctggaa
     1561 gttgtccgcc cgggcggcct ctacctccat gtccaccatc aaagcacgat catggaagtt
     1621 gatgttgtcg gcgatgcctg tgacaacaag gcctgccatg gtggcgatca ggccggctgt
     1681 gacgcgtacc gccatggacc gcggttcgcc cgggggggcg ggccggtgcc cggtgtcttg
     1741 tggaagtgtg atgatgcgtg ctgcccatgc gaacagcgca ccaatgagca atgcgagcag
     1801 gagaggggcg gagtacggcg gtgaactgtc catgacctcg gaggagaggg ctcccaggtc
     1861 gatcgtactg gcgaggtatg gatcggtgcc ggtacatttg atcggcacga tgttgcgcat
     1921 ggtgtccact tccagggtgc gcaggtcatt cgcggccccg aagcggtccg tgatgtcccg
     1981 tccttcgagg cgcttggtgc ggaacggtcc gcgcaattcg atggccttca gcgtcatttg
     2041 gaccgcttgc gtgccgggat cgatgcgaag gccgcgcacg cggtgtacgc tgtcgggcaa
     2101 ttggaaccgg atccgttgga ggtcatacgc cggcatcacc gtccggttca gcatgcgatc
     2161 gggttcgtat ccgctcgatc cattgtccca gaacaattcg aatttgtcag ggcaggtggt
     2221 cgtcacctcc agtgtcacct ccaagccgcc aggcagggca cgtggccgca gggcccacca
     2281 cgcgagagct gccgtcagca ccccgatggt aaagggcaac catgttcgga aggtggtgcc
     2341 gcgcatggtt cagaagcgga aatagatgaa cggggagaag gtgctgctcg ccacctgcat
     2401 ggccacgccg atgaacagga ccaacaggcc aaagagttgg accgcgctcc agacgccctg
     2461 cggtggaacc tcgccgctga acgccgggag gcggagcatc ctgccgatgg ccacatgcgc
     2521 attgaccgag ccggccacgc cgagcaccaa ggccagtagc gtaacgttgc tcaggtacaa
     2581 ttccgggtag gtgagcaggc catcgcctgc ggacgggttc cacatcgcgt tcgcgaagcg
     2641 ccatgctccg gtgatgtcct cgatgcggaa gaacgcccag gcggtaagca ccacggcgag
     2701 ggtgtacacg tgcccgacga accggggcag acgatccagt acacgcccca gtccggcccg
     2761 ttcggccatc atgaacgcac cgtggatcag cccccagacc acgaagttcc aactggcgcc
     2821 gtgccacagg cctgtcagcg tgaaaataat caataggtta aggtatgttc gcacgggtcc
     2881 gccccggttg ccgccgaggg ggatgtacac gtagtcgcgg aagaagttgg tgaggctgat
     2941 gtgccatcgt ttccagaatt cacggatact ggtggcgata taggggcggt tgaagttctc
     3001 caggaagcgg aagccgaaca tgcgggctga gccgatggcc atgtcgctgt aaccgctgaa
     3061 gtcgaagtag atctgcacgg cgtaggcggt gataccgagc caggccacgg gcatggtcac
     3121 atcatccggt acctcgtgga agatggggtc ggcgatgcgg gcgcattgat cggcgatgag
     3181 gaccttcttg gccaggccga tgatgaatcg gcgcacgccg tacgcgaagc cgctgacgtc
     3241 catgcgccga tcggtgaact ggtccgccac atcatgatag cggatgatgg ggccggccac
     3301 caactgcggg aacagggcga tgtagagcag cgtgatgccc gggctgcgct gcgccttcgc
     3361 atcgccgcgg aacacgtcca cggtataact caggctgtgg aagatgaaga aggagacacc
     3421 gatcggcagg tgtacgggat ccaggagcag gatatcggaa ccgagcaggg cattgatggt
     3481 ggtggcgaag aggttcgcgt atttgaacca gatgagcacg gccaggttgg cgaacacggc
     3541 aagagccagc acggcgcgcg gcgatcgcca ccgctcgatg gccaggccgc acagatggtt
     3601 gaacagcgcg gtgcccacca tcagcaacac ccacgacttc tccccccaag cgtagaagaa
     3661 caggctggcg atgagcagca aggtgttctg ccgcttccgg tcgccggcca ggagcaccag
     3721 caggagcgtg agcggcagga attggaagag gaagaccggc gaggagaaga ccatggaccc
     3781 gagggggcac gaatgtagac ggacccgcca tggggagggt ccgtccgttg gtgtcgttac
     3841 gtggcagggc gccgttgagg cctactgcgg aatggcatac tcacgcgtca tcatcatcgt
     3901 ccttctcatc atccccggca tcgtcatcgt cgtcgtcacc atcgacatcg tcgtcatcgc
     3961 ccgtatcgtc gtcatcctca tccgcgtcct cccctccggg cgtttcgatg tcctccccgt
     4021 cgcgggtatc catgtcctcg gccatgttgc cgccgccctc ctcctggtcg aggaactgct
     4081 cgatctcctc gcggctttcg gggttgatct tgatgaggta gatggcgtcg gaggtgcgca
     4141 gttcgatcac gcggaggctt tcacctttgg gcgtgttcag ggtgcggatg tggctgttgt
     4201 cgatgccgtc ggggtactgc tcctgcagca tctcgcgcag gtcatcggtc aacgtgttga
     4261 aggagcggat cagtcgctgc atgctgtgaa cgccgaagct cggcgaaggc gttggaggga
     4321 ggcttacagg tccaggatgt atgcgaatat gagcggggcc acaatagtag catcgctctc
     4381 gatgatgaat ttcggggtat cgatgtcgag tttgccccag gtgatcttct cgttgggcac
     4441 ggccccgctg tagctgccga agctggtggt gctgtcgctg atctggcaga agtagctcca
     4501 gaaggggatg ctgtcgcgtt ggaggtcctg gtgcagcatg ggcaccacgc agatggggaa
     4561 gtcgcccgcg ataccgccgc cgatctggaa gaaaccgatg ccgtcggtgc cgctgttgcg
     4621 ggtgtaccaa tcggccagga acatcatgta ctcgatgccg ctcttcatca tttgcggctt
     4681 gcagtcgccg acgatgcagt ggccggcgaa gatgttgccc agggtgctgt cctcccagcc
     4741 ggggcagatg atcggcaggt cgcgttcggc ggcggccacc atccaactgt ccttcgggtc
     4801 gatctggtag tattgttcca gcacgccgct ccggatcatc tcgtaaaaat actggtgcgg
     4861 gaagcggcgg ttgccgctct tgt
//
LOCUS       DAOQOC010000065         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7394_length_4850_cov_3.504484, whole genome shotgun sequence.
ACCESSION   DAOQOC010000065 DAOQOC010000000
VERSION     DAOQOC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7394_length_4850_cov_3.504484"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PL106_01730"
     CDS             <1..347
                     /locus_tag="PL106_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31816.1"
                     /translation="FCVIMIEAAANEGRPIEEYLLTERFTLAGMPHLKSDRYKAHMRA
                     VEVDDENGLRKLIRSMATKGWVEYNNDDTFRFLRPFHRLFDKCQEISQAAEQENRDVL
                     PGLEPKIDPEMN"
     gene            397..3147
                     /locus_tag="PL106_01735"
     CDS             397..3147
                     /locus_tag="PL106_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31817.1"
                     /translation="MQNGLGPSKLIAIRSGSYDYAEVDLATSIQLVGPNNAGKTTLIN
                     TLQFLYLDNRNQMNFGDHDPEATRAYYFPDQFSYLLFELEGPKGTYVLGWRGQSKAQG
                     GDPLRFTYDGPYNVNDYLTDDGRVQDQKLVDARLSDRNFRVLRDAAAHRESILVATKG
                     ESNGLGVVHLTETERYAHFKEVLKNLLSLRSIDQHEMKRSLLMLAGIPPSRPALEANR
                     LMTEDFERIRDRRLKLNTLKSERERLEKYIDMSDVRFALEARLAFVYADLMEKRRKAH
                     DHNKHVKEAIVAKQEALEKEKISTAGILKDLDEKIAEIAGFKGRVEGDLSRLADLGKG
                     LEQFVENLEREALHNAKERLHLLNRQLGNAEEADRQRTARALAEVTARVASTERAIAN
                     FDKALITELRETMDDEALHGLSKLFNLEILKQPVGPSTGSGSTDHVVLKKRKQIDALL
                     KGIAENITNDKYSDALMSLPLPDNSTAWKELLDVKALKKALSDLKAEQDRLRKLMDAI
                     ENRETLAKDRAAVQSECDAHTLNLNRWESLQRERVEEPRLQKRLSELTKEKTEGDRER
                     KEVRDKLEEIGVKLYEQRTALNKEDERFNRLLQLMEQCVPPPDPEAKGKEPVSAPAEA
                     EDAIALYGKLLQEHNAMSREMDQLRSKIESVLKSDIIGPNEQETVRLMREQIDGLPTF
                     EETLRKDWDNYLHLLRANFQNVLNGLNDIKQAAEQLNRRFTNVSVSNLESLRMEVMDQ
                     NELVAALKELAETDHTGLFDSSGKLDAAYQSFRNKLSERLTLNYGDLFTLRFTVSTRS
                     GRVHSYDNLQVESHGTAITIKVLFNLLVLRSMLKEDPKLRTPLSRVPFFLDEVHSLDA
                     INRKAVLSMAKDLGFMAITAAPEPVSEVEALYFLRPINGRLVVRNELRIGVKFDEAAV
                     AS"
     gene            3236..4186
                     /locus_tag="PL106_01740"
     CDS             3236..4186
                     /locus_tag="PL106_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31818.1"
                     /translation="MAGSPRFMIKVASLCTTLALAPCVFAQDELPTSVDIDLRMNAND
                     STELEVYLRSNDLGFSDVVAGLTFTIRWTTTSPAALGTRTNNCPDGIPIAPVTQETNP
                     DLNGTPTGFNYRTYNGFGTSLLSEWGCLMPQDEWYLVMSVPVVNNDGCTLFEIVNDPW
                     TAVNNRDYYISLGGYERQGIIEPTGVPMGTCATDCLGQVGGTALPGTPCDDGNAETDN
                     DVYTAECVCQGDPSTIIHVQETMPALLLWPNPTNGILNVNGATVVGVSDVLGRRAQLP
                     TVRAKSPGISTMDLSGLPTGVYLVELEQDGARRVERVVKR"
     gene            4337..>4850
                     /locus_tag="PL106_01745"
     CDS             4337..>4850
                     /locus_tag="PL106_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HNA31819.1"
                     /translation="MRRSTIAPVFALIASFTLSAKEQPVTSKVSAAKVFLSGAQVDRT
                     ASATISSGSTTLIFTGLSQYLDPQSIQVNGKGGFSILSVNHRINYLSESPKKKEIDDL
                     EKRITKLEKDRSYEKAMQDVWVNEEQLLNKNSSIGGQQNGLTAAQLTAVNDYVRTRLQ
                     VTKTNWLAQQE"
BASE COUNT         1098 a         1564 c         1434 g          754 t
ORIGIN      
        1 tgttctgcgt catcatgatc gaagccgcgg ccaacgaggg acgtcccatc gaggaatacc
       61 tcctcaccga gcgcttcacc ttggcgggca tgccgcacct gaagagcgac cgctacaagg
      121 cacacatgcg cgcggtggag gtggacgacg agaacggcct gcgcaaactc atccggagca
      181 tggccaccaa gggctgggtc gagtacaata acgacgacac cttccgcttc ctccgcccct
      241 tccatcggct cttcgacaaa tgccaggaga tcagtcaggc cgccgaacag gagaaccgcg
      301 atgtgcttcc gggactggag ccgaagatcg atccggagat gaactgatcc cgatcgcgga
      361 acgaccacac acgacttacg gcagaaaaac cgatacatgc agaacggact aggcccctcc
      421 aagctcatcg ccatccgcag cggcagttat gattacgccg aagtggatct cgcgacctcg
      481 atccagctcg ttggccccaa caacgccggg aagaccacgt tgatcaacac gctgcagttc
      541 ctctacctgg acaacaggaa ccagatgaac ttcggcgacc acgaccccga ggccacgcgc
      601 gcgtactatt tccccgacca gttcagctac ctgctgttcg agctggaagg gcccaagggc
      661 acttacgtgc tcggctggcg cgggcagagc aaggcccaag gcggcgatcc gctgaggttc
      721 acctacgacg gaccgtacaa cgtgaacgac tacctcaccg atgacggtcg tgtgcaggac
      781 cagaaactcg tggacgcccg cctgagcgat cgcaacttcc gcgtgctgcg cgatgccgct
      841 gcgcaccgcg aatcgatcct ggtggccacc aagggcgaga gcaacgggct gggcgtggtc
      901 caccttaccg agaccgaacg ctacgcgcac ttcaaagagg tgctgaagaa cctgctcagc
      961 ctgcgcagta tcgatcagca cgagatgaag cgcagcttgc tcatgctggc gggcatcccg
     1021 cccagcaggc ccgcgctgga ggcgaaccgc ctgatgacgg aggacttcga gcgcatccgc
     1081 gatcgccgcc tcaaactgaa cacgctgaag agcgagcgcg agcgcctgga gaagtacatc
     1141 gacatgagcg atgtgcgttt cgccctggaa gctcgcctgg ctttcgtgta cgccgacctg
     1201 atggagaagc gccgcaaggc tcacgaccac aacaagcacg tgaaggaggc catcgtggcc
     1261 aagcaggagg cgctggagaa ggagaagatc agcacggccg ggatcctcaa ggacctcgac
     1321 gagaagatcg ccgagatcgc cggcttcaag ggccgtgtgg aaggtgacct gtcgcgactc
     1381 gccgacctgg gcaagggcct ggagcagttc gtggagaacc tcgaacgcga agcgttgcac
     1441 aacgccaagg agcgcctgca cctgctgaac cgccagctgg gcaatgccga ggaagccgac
     1501 cgccagcgca ccgcgcgtgc cctcgccgaa gtgaccgccc gcgtggccag caccgagcgc
     1561 gccatcgcca acttcgacaa ggcgctgatc accgagctgc gtgaaacgat ggacgacgag
     1621 gcgctccatg gcctcagcaa actcttcaac ctggagatcc tcaagcagcc cgttggccct
     1681 tcgacaggct cagggtccac ggaccatgtt gtactgaaga aacgcaagca gatcgacgcg
     1741 ttgctgaagg gcatcgccga aaacatcacc aacgacaagt acagcgatgc gctcatgagc
     1801 cttccgctgc cggacaacag cacggcgtgg aaggaactcc tcgatgtgaa ggcgctgaaa
     1861 aaggcgttga gcgacctgaa ggccgagcag gaccgcctgc gcaagctgat ggacgccatc
     1921 gagaaccgcg agaccctggc caaggaccgc gccgccgtgc aatcggagtg cgatgcgcac
     1981 acgctcaacc tgaaccgctg ggagtcgctc cagcgcgagc gtgtggagga gccgcgcctg
     2041 cagaagcgac tgagcgaatt gaccaaggag aagaccgagg gcgaccgcga acgcaaggag
     2101 gtgcgcgaca agctggagga gatcggtgtg aagctgtacg agcagcgcac cgccctgaac
     2161 aaggaggacg aacgcttcaa ccgcctgctg caactgatgg agcagtgcgt gcccccgccc
     2221 gatcccgaag cgaagggcaa ggagcccgtg tccgcccccg ccgaggccga ggacgccata
     2281 gccctctacg gcaagctcct gcaggagcac aacgccatga gccgcgagat ggaccagctg
     2341 cgcagcaaga tcgaaagcgt gctcaagagc gacatcatcg gccccaacga gcaggagacc
     2401 gtgcgcctga tgcgtgaaca gatcgacggc ctgcccacct tcgaagaaac actgcgcaag
     2461 gactgggata attacctgca cctgctgcgc gccaacttcc agaacgtcct caacggcctc
     2521 aacgacatca agcaggcggc cgaacagctc aaccgccgct tcaccaacgt gagcgtctcg
     2581 aacctcgaga gcctgcgcat ggaggtgatg gaccagaacg aactcgtggc ggcactgaag
     2641 gaactcgccg agaccgacca caccggcctc ttcgacagca gcggcaaact cgacgcggcg
     2701 taccagagct tccgcaacaa gctcagcgag cgcctcacgc tcaactacgg cgacctcttc
     2761 accctgcgct tcaccgtgag cacacgttcg ggccgcgtac acagctacga caacctgcag
     2821 gtggaaagtc acggtaccgc gatcacgatc aaggtgctct tcaacctgct cgtgttgcgc
     2881 agcatgctga aggaggatcc caagctgcgc accccgctca gccgcgtgcc cttcttcctc
     2941 gacgaggtcc actcgctcga tgccatcaac cgcaaagccg tgctcagcat ggccaaggac
     3001 ctcggtttca tggccatcac cgccgcaccc gaaccggtga gcgaggtgga ggctctttac
     3061 ttcctgcgcc cgatcaatgg acgtctggtg gtgcgcaacg aattgcgcat cggtgtgaag
     3121 ttcgacgaag ctgccgtggc ctcataagag cctgtcgttc atcccggtat ttcaggcgcc
     3181 cattcccacc aagggggtgg gcgccgtata ttagcggtca cgaaacggta ttcccatggc
     3241 aggttctcca cgcttcatga tcaaggtcgc atcgctttgt accacgctcg cactcgcccc
     3301 ctgtgtcttc gcccaggatg agttgcccac gtccgtggac atcgatctgc gcatgaacgc
     3361 gaacgactcc acggagttgg aggtgtacct gcgctccaat gacctcggtt tctcggacgt
     3421 ggtggccggc ctcaccttca ccatccgatg gacaaccacc agcccggccg cgctggggac
     3481 gcgcacgaac aactgcccgg acggcatccc catcgcacct gtcacccagg aaaccaaccc
     3541 cgacctcaac ggcacgccca ccggcttcaa ctacaggacc tacaacggct ttggtaccag
     3601 tttgttgagc gaatgggggt gcctgatgcc ccaggatgaa tggtacttgg tgatgagcgt
     3661 tcccgtggtg aacaacgacg gctgcaccct cttcgagatc gtgaatgacc catggacagc
     3721 ggtcaacaac cgcgattatt atatctcgct cggtggatac gagcggcagg ggatcattga
     3781 acccacaggc gtgcccatgg gaacctgtgc caccgattgt ctgggacaag tgggcggaac
     3841 cgcgctcccg ggcacccctt gcgatgatgg caatgcggaa acggacaatg acgtgtacac
     3901 cgctgaatgc gtctgccagg gcgacccctc cacgatcatc catgtccagg aaacgatgcc
     3961 tgcccttctg ctgtggccca atcccaccaa cgggatcctc aacgtgaacg gtgccaccgt
     4021 ggtcggtgta tccgatgtgc ttgggcgccg cgcccaactt ccgaccgttc gagccaagag
     4081 cccgggtatc agtaccatgg acctctcggg gctgccgacc ggggtgtacc tggtggagct
     4141 tgagcaggat ggtgcccggc gtgtggagcg cgtggtgaaa cgctgatcca aaggatccgt
     4201 aggggcccct gcgggtccgt gcggaaccgc ctgctggtgg gcgaaaggag ttgatcgaac
     4261 cttgtggcac gttacgtgta ccgctccccg gtaccgcaca aacgcggttt tcctttgcac
     4321 catgttcatc caccacatgc gccgctccac catcgccccg gtcttcgcac tcatcgcgtc
     4381 attcaccctc tccgccaagg aacaacccgt caccagcaag gtctcggcgg ccaaggtctt
     4441 cttgagcggc gcacaagtgg accgtaccgc gagcgcgacc atcagctccg gtagcacaac
     4501 gctcatcttc acgggcctca gtcagtacct cgatccgcag agcatccagg tgaacgggaa
     4561 aggcgggttc agtatcctga gcgtgaatca ccgcatcaac tacctgagcg aaagcccaaa
     4621 gaagaaggag atcgacgacc tggaaaaacg gatcaccaaa ctggagaagg accgctcata
     4681 cgagaaggcc atgcaggacg tgtgggtgaa cgaggagcaa ctgctcaaca agaacagcag
     4741 catcggcggg cagcagaacg gcctcactgc cgcacaactc actgcggtga acgactatgt
     4801 gcgcacccgc ttgcaggtga cgaagaccaa ctggctggca cagcaggaga
//
LOCUS       DAOQOC010000066         4839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7421_length_4839_cov_3.645903, whole genome shotgun sequence.
ACCESSION   DAOQOC010000066 DAOQOC010000000
VERSION     DAOQOC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7421_length_4839_cov_3.645903"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PL106_01750"
     CDS             <1..450
                     /locus_tag="PL106_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31820.1"
                     /translation="NPYTLQPGVQFNGFYADVMPQGLTSDHSFLFVWYDVADTTDSVS
                     VRIDFEIAEGAGIAERTDVVNTFSAMPNPSLDGAVNLDIDLAKWSGHEELMLRDATGR
                     ISMRRNITQGTRRVDLQAGELAPGVWMATLLSDGAVLATRRLVVAGH"
     gene            complement(444..1157)
                     /locus_tag="PL106_01755"
     CDS             complement(444..1157)
                     /locus_tag="PL106_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNA31821.1"
                     /translation="MEIVFFTGAGISAESGIRTFRDSDGLWEEYRVEDVATPQAFARD
                     PALVLHFYDLRRAQVLKAEPNAAHRAIAVLETHRDHRGQRPFTVRVVTQNVDDLHERA
                     GSSHVLHLHGEILKARSTKDARIILPINGPSLALGDRCPLGSQLRPHIVWFGEEVPLL
                     AEAADIVERADVLVVVGSSLQVYPAAGLVLHARPDCPIHVVDPAGANTAIRRVQHWKD
                     KASSGVPELARLLISEYGQ"
     gene            1388..1999
                     /gene="ruvA"
                     /locus_tag="PL106_01760"
     CDS             1388..1999
                     /gene="ruvA"
                     /locus_tag="PL106_01760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNA31822.1"
                     /translation="MIDSLRGEIAEKAPGHVVVECHGVGYLVLVTANTLGGLPAQGPC
                     KLFIHYAVSVDVRSGQSEHKLFGFISTEERHMFRQLIGVQGVSATLGLAILGARQTDE
                     LRVAILNGEEHMLKAVKGIGPKLAQRIVTELRGKLADEPVQQVLAVAGGGGNTLRAEA
                     LSALVSLGLDRARAERALQKSLAEREGDTPPLEELIRTALKNL"
     gene            2010..>4839
                     /gene="sprA"
                     /locus_tag="PL106_01765"
     CDS             2010..>4839
                     /gene="sprA"
                     /locus_tag="PL106_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNA31823.1"
                     /translation="MHLAHPHLIHVLFTWGPRLLAILAVGMLSGKRLMAADNAGTHLA
                     EFALAQQVDSPQIDLPYPIVDPPDPGNTNTGTIDLAPPGNVTNEVVYDPVTGQYIMQS
                     NIGDNIQYRPPMSMSLDEYLNYDMQKSMKTYWAEKNKENAERETNNPLIKSIAVKSEL
                     FCRIFRGCNIDIRPQGSAEITFGLNISKTENPRIPVEQRKITTFNFDQRIQLNLTGNI
                     GEALKINTSYNTEATFDFENQVKLNYVGDEDQIIQKLEAGNVSLPLSGQLIQGSQSLF
                     GVKTELRFGRLTATGIFSQEKGQRRNVQVAGGAQTTNFDIKADEYEANKYYFLSNFFR
                     DNYENALRTLPLVNSGINITRIEVWITNTRFDYQQNRNIIGFTDLGEDGSPQAIAAGH
                     FSSDLPGEVVDAAGNLADNGANSLFGIMSGAGGPGNSFGNATAFLQSGGYQASRHYEK
                     LESARMLSPNEYTLNERLGFIGLNQNLNNDEVLMVAYQYTLDGVTHQVGQFGSDVTDQ
                     SKALILRLLKATITNPRIPLWDLMMKNVYSLGAFQVNRENFRLDLVYNNPTTGVDINY
                     VPRPPLDQIPVIQALGLDRLDPQNAPNPDGWFDFLDGAATTGGTINTQNGRIFFPVLE
                     PFGSYLDRRLQENPANTEQIRRNIVYQQLYDSTKTAAQNIPELNRFRLKGSYKSASSD
                     VISLNSVNIPQGSVTVTAGGVRLVEGQDYTVDYNLGRVKILNQGILESGTPINISLES
                     NSLFSIQQRTLGGVRLDYKVSKDLQIGATAMTLYERPLTQKVNVGDEPIANTIVGLDA
                     NWRTESNLITKLVDQLPFYATKEVSKVDASIEGAYLIPGHSRAIGNMGTSYIDDFEGS
                     VSYIDMRQQAQWFHAATPQGQPDLWPEGEQFGRPNGSRRAHLAWYVVDPIFFRDNSLK
                     PPLPDGGDRLNMTRQVLEAEVFPNRQLPT"
BASE COUNT         1024 a         1558 c         1474 g          783 t
ORIGIN      
        1 aacccctata ccctgcagcc cggtgtgcag ttcaatggtt tctatgcgga tgtgatgcct
       61 caaggcctca ccagcgacca ctccttcctt ttcgtgtggt acgatgtggc cgataccacg
      121 gacagcgtat cggtgcggat cgatttcgag atcgccgaag gtgccgggat cgcggagcgc
      181 accgatgtgg tgaatacgtt cagcgccatg cccaatcctt cgctggacgg tgcggtgaac
      241 ttggacatcg acctggcgaa atggagcggc cacgaggagt tgatgctgcg cgatgccacc
      301 ggccgcatca gcatgcgcag gaacatcacc caagggacgc gccgcgtgga tctgcaggct
      361 ggcgagctgg cccccggcgt gtggatggcc acgctgctga gcgatggtgc ggtgctcgcg
      421 acccgccggt tggtggtcgc cggtcactga ccatattccg agatcaacag acgggccagt
      481 tcgggtacac ctgaactggc cttgtctttc caatgttgta cgcgcctgat cgcggtgttg
      541 gccccggcgg gatccaccac atggatcgga cagtccggcc gcgcatgcaa gacaaggccc
      601 gccgccgggt acacctgtag cgaggagccc accaccacca ggacatccgc ccgttccacg
      661 atgtccgcgg cctcagccag caggggcacc tcttcgccga accacacgat gtggggtcgg
      721 agttgggatc ccaacggaca acgatccccc aacgcgagtg atggaccgtt gatcggcagg
      781 atgatacgcg cgtctttcgt gctgcgggct ttgaggatct ccccatgcag gtggagcacg
      841 tgcgagcttc ccgcccgctc gtgcagatca tccacattct gcgtcaccac cctaacggtg
      901 aagggccgct gcccgcggtg atcccggtgc gtttccagca ccgcgatggc ccgatgggcc
      961 gcattgggct ccgccttcag gacctgcgcg cgccgcaaat cgtagaaatg caatacaagt
     1021 gcggggtccc gggcgaaggc ctgtggtgtg gccacatcct ccacgcggta ctcctcccac
     1081 aggccatcac tgtcgcggaa ggtgcggatg ccgctctcgg cactgatacc cgccccggta
     1141 aaaaacacga tctccatagg atcatccccc accggttcat cggtccttgg gtcacgcgaa
     1201 catggggttt tctgtcggga acctgcacca accatacaag gcgcgtgcat gcatggccgc
     1261 tgcgtcctat tttggcgccc ctttctacgg gaccctctgt tgcaggaggc cctcaccctg
     1321 aaccccacgc catcgaagag acgctactcc ccttcttacg gagcacagaa ctgacggagg
     1381 gtcaggaatg atcgacagct tacgcggcga gatcgcggag aaagcgccgg gccatgtcgt
     1441 ggtggaatgc catggcgtgg gctacctcgt gctggtcacg gccaacacgc tcggtggcct
     1501 ccccgcgcaa gggccgtgca agttgttcat ccattacgcg gtcagtgttg atgtgcgcag
     1561 cgggcagagt gagcataaac ttttcggatt catcagcacg gaagaacggc acatgttcag
     1621 gcagttgatc ggggtacaag gcgtgagcgc cacgctcggg ctggccatcc tgggggcacg
     1681 gcagacggac gagttgcgtg tggccatcct gaatggggag gagcacatgc tcaaggccgt
     1741 gaagggcatc gggccgaagc tggcccagcg catcgtaacg gaactgaggg gaaaactcgc
     1801 cgacgagccc gtgcaacagg tattggccgt ggctggtggt ggaggcaata cgctccgggc
     1861 tgaagcgtta tcggctctgg tttcactggg gctggaccgg gccagggccg agcgtgcctt
     1921 gcagaagtcg ctggcggaac gggagggcga taccccccca ttggaagagt tgatcaggac
     1981 cgcgctgaag aacctttgac catcccgccg tgcacctcgc gcatccgcac ctgatccatg
     2041 tcctgttcac ctggggaccg cgcctgctgg cgatcctggc cgtgggcatg ctcagcggca
     2101 agcggctcat ggccgctgac aacgccggaa cgcacctggc cgaattcgct ctcgcacagc
     2161 aggtggacag cccgcagatc gacctgccct accccatcgt ggatccaccc gatccgggca
     2221 acacgaatac cggcacgatc gacctggcgc cgcccgggaa tgtgaccaac gaagtggtgt
     2281 acgatccggt caccggccag tacatcatgc agagcaacat cggcgacaac atccagtacc
     2341 ggccgcccat gagcatgtca ctggatgagt acctcaacta cgacatgcag aagagcatga
     2401 aaacgtactg ggccgagaag aacaaggaga acgctgaacg cgagacgaac aatccgctca
     2461 tcaagtccat cgccgtgaag agcgagctct tctgccggat cttccgcggc tgcaacatcg
     2521 acatccggcc gcagggcagc gccgagatca ccttcgggct caacatcagc aagaccgaga
     2581 acccgcggat cccggtggag cagcgcaaga tcaccacctt caacttcgac cagcggatcc
     2641 agttgaacct cacgggcaac atcggtgagg cgctcaagat caacacctcg tacaacaccg
     2701 aggccacgtt cgacttcgag aaccaggtga agttgaacta tgtcggcgac gaggaccaga
     2761 tcatccagaa gctggaggcc ggcaacgtga gcctgcccct gagcggccag ctgatccagg
     2821 gcagccagag cctcttcggg gtgaagaccg aactgcgctt cgggcgcctt accgcgaccg
     2881 gtattttcag ccaggagaag ggccagcggc gcaacgtgca ggtggccggc ggggcgcaga
     2941 cgaccaactt cgacatcaag gccgacgagt acgaagccaa caagtattat ttcctcagca
     3001 acttcttccg cgacaactac gagaacgccc tgcgcaccct gcccctggtg aacagcggga
     3061 tcaacatcac gcgcatcgag gtgtggatca ccaacacacg cttcgactac caacagaacc
     3121 gcaacatcat cggattcacc gacctgggtg aggacggcag tccccaggcc atagcggccg
     3181 gccatttcag cagcgacctg cccggcgagg tggtcgatgc ggcgggcaac ctggccgaca
     3241 acggggccaa ctcgctcttc ggcatcatgt ccggtgccgg agggcccggg aacagcttcg
     3301 gcaacgccac cgccttcctg cagagcggcg gctaccaggc cagccggcac tacgagaagc
     3361 tcgagagcgc gcgcatgctc tcgcccaacg agtacaccct gaacgaacgg ctcggtttca
     3421 tcggcctgaa ccagaacctc aacaacgatg aggtgctcat ggtggcgtat cagtacaccc
     3481 tggacggcgt cacccaccag gtgggccagt tcggctcgga cgtcaccgat cagagcaagg
     3541 cactcatcct gcgcctgctg aaggccacca tcaccaaccc gcgcatcccg ctgtgggacc
     3601 tgatgatgaa gaacgtgtac tccttggggg cgttccaagt gaacagggag aatttccggc
     3661 tggacctggt gtacaacaac cccaccacgg gggtcgacat caactacgtg ccgcggccac
     3721 cactggacca gatcccggtg atccaggcgc tgggcctgga ccggctcgat ccgcagaacg
     3781 cgcccaaccc cgatggctgg ttcgacttcc tggacggtgc ggccaccacg ggcgggacca
     3841 tcaacaccca gaacggccgc atcttcttcc cggtgctgga accgttcggc agctatctgg
     3901 accggaggtt gcaggagaac cccgcgaaca ccgagcagat ccggcggaac atcgtgtacc
     3961 agcagttgta cgacagcacc aagaccgccg cgcagaacat ccccgaactc aaccggttcc
     4021 gcctgaaggg cagttacaag agcgccagca gcgatgtgat cagcctcaac tcggtcaaca
     4081 tccctcaggg gtcggtgacc gtgacggccg gcggggtacg gctggtggaa ggccaggact
     4141 acacggtgga ctacaaccta ggccgggtga agatcctcaa ccaggggatc ctcgagagcg
     4201 ggacacccat caacatctcc ctcgaaagca actcgttgtt cagtatccaa caacgtaccc
     4261 tcgggggggt gcggctcgac tacaaggtga gcaaggacct ccagatcggg gccaccgcga
     4321 tgacgttgta cgaacgtccg ctcacccaga aggtgaacgt gggcgacgag cccatagcca
     4381 acacgatcgt ggggctggac gccaactgga ggaccgagag caacctgatc acgaaattgg
     4441 tggatcaatt accgttctac gccaccaagg aggtcagcaa ggtggacgcc agcatcgagg
     4501 gtgcgtacct gatccccggc cacagtcgtg ccatcggcaa catgggcacg agctacatcg
     4561 atgatttcga gggcagcgtc agttacatcg acatgcgcca gcaggcccag tggttccatg
     4621 ccgccacacc gcagggccag cccgacctct ggcccgaggg cgagcaattc ggcaggccca
     4681 acggatcccg gcgggcgcac ctcgcctggt acgtggtgga cccgatcttc ttccgggaca
     4741 acagcctgaa accacccctg cccgacggcg gcgaccggct gaacatgacc cgccaggtgc
     4801 tggaggccga ggtcttcccg aaccgccagc tgcccaccg
//
LOCUS       DAOQOC010000067         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7505_length_4805_cov_3.647158, whole genome shotgun sequence.
ACCESSION   DAOQOC010000067 DAOQOC010000000
VERSION     DAOQOC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7505_length_4805_cov_3.647158"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PL106_01770"
     CDS             complement(<1..565)
                     /locus_tag="PL106_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNA31824.1"
                     /translation="MNDLLQKLSTVHDRRTEVGKRLADPAVIADQQQFVVLSREYRDL
                     EPIEEAYLRYKRAFEGLEGAREILRTEKDPDLLDMARTEQSELQAALEAMDEEVRMLL
                     VPKDPNDARNCTMEIRAGTGGDEASIFAGDLFRMYTRYCEGRGWKVEVADASDGSVGG
                     YKEVVVNVIGDGAYGTLKFEGGVHRVQR"
     gene            complement(601..1773)
                     /locus_tag="PL106_01775"
     CDS             complement(601..1773)
                     /locus_tag="PL106_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNA31825.1"
                     /translation="MTGNDRYTRRGVSADKEDVHRAITGLDKGLFPKAFCKVVPDSLV
                     GSPDHCIVMHADGAGTKSALAYAYWKETGDLSVWHGIAQDAVVMNLDDLLCVGITDNI
                     LLSSTIGRNKRLIPGEVIAAIIEGTELFLERMRGLGIGITSTGGETADVGDLVRTVIV
                     DSTVTARLRREDVIDNARIRGGDIIVGLASSGQATYEDGHNAGMGSNGLTSARHDVFT
                     KAVGERYPETFDPGTPEALIYAGRAGLTDPLHGTPLDFGKAVLSPTRTYAPVIREVLK
                     AMRADIHGMVHCSGGAQTKVLHFIEGLRVIKDALFPVPPLFAAIQRMSGTDWREMYKV
                     FNMGHRMELYVPEARAQEIIGISRTFGIEGRIIGRVEAAATKEVVIAGENGTFSYT"
     gene            complement(1835..3844)
                     /locus_tag="PL106_01780"
     CDS             complement(1835..3844)
                     /locus_tag="PL106_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA31826.1"
                     /translation="MRKILATAALSLLCTTLAVAQNKQAEEADAAYKAGQYFNAIEMY
                     KKAYTSEKKASEKAILIYKVAECYRVLGDAPNAQVWYEKANKAQYTDPITYYWIGEAL
                     KEQGKYAEAISAYNKYKEKNPGDKRADASLAACQLAQTWKDSPSRYTVDPEVLLNSPQ
                     YDMSATFSVKKNDEVVFASSRPASTGTETDGIAGESYLDLFASSRDKLGKWSEPVKLP
                     PTINTPAHEGSATFNAKRTVMYFTRCEHEKKKAFGCEIWMTKKVGSNYSEPEMLVLHA
                     AAGKLDTNQYKLGQPTLSKDDGIMVYASNQKGGQGGKDLWYIRLDKDGKPTGTPTNLG
                     PEINTKKDEYFPFLRENGNLYFSSDGHPGMGGLDIFAAEKTGDNTWGHVENVKSPINS
                     SGDDFSIIFDGAEEKGYFTSNRPGGKGQDDIWRFMMPNLEFALQGTCYDKLTGNVLPD
                     TKVEVVGTDGSSFSALTDANGGFVFTEKGKDRYIKENTSYTIRASKDKYLVVNDQITT
                     VGLNESTTFAKEYYLQPVIENKGIPLPQVLYEVDKFDLTQAGKDSLETLYNTLVENPT
                     IIIELRSHTDSRNTRTYKGGNMELSQKRAQSCVNYLVTKGIDPVRMVPVGKGATEPIV
                     TDKEIKAMKTKEEQEAAHQKNRRTDFTVLSFDYVPKEGGTPSPNQ"
     gene            complement(4086..>4805)
                     /locus_tag="PL106_01785"
     CDS             complement(4086..>4805)
                     /locus_tag="PL106_01785"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HNA31827.1"
                     /translation="TIVASQLRAFKKTVDAMIKKGTKKDEAILRVIRDLIVESKAIRF
                     EGNGYGDEWKAEAKKRGLSNIADTPRALDVWERAETKKLFSEMGVLSEVELEARHEIE
                     LHSYVLKLQIESRVAGDLAVNHIIPAAISYQNRLIENVRGLKDVLGPEKAAKAAATQI
                     GMIEEISEHLGRLKTLTDDMIEARKKANVIEDHREQAIAYCDQVKPYLDKIRYHANKL
                     EINVDDELWPLPKLRELLFTR"
BASE COUNT          741 a         1509 c         1426 g         1129 t
ORIGIN      
        1 cacgctgcac ccgatggacc ccaccctcga acttgagcgt accgtacgca ccatcgccga
       61 tcacgttgac gaccacttcc ttgtatccgc ccaccgagcc atcgctcgca tcggccactt
      121 ccactttcca tccgcggccc tcgcaatagc gcgtgtacat gcggaaaaga tccccggcga
      181 agatgctggc ctcgtcccca ccggtacctg cccggatctc catggtgcag ttgcgcgcat
      241 cgttcggatc cttgggtacc aacaacatgc ggacttcctc gtccatggcc tccaaggccg
      301 cctgcagttc ggactgctcg gtgcgggcca tgtccaacag gtcgggatcc ttctcggtcc
      361 gcaggatctc acgggcccct tccaggccct cgaacgcacg cttgtaccga aggtatgctt
      421 cctcgatcgg ctcgagatca cggtactccc gactgagcac aacgaactgc tgctggtcgg
      481 cgatcaccgc cggatcagcg aggcgcttgc ccacttcggt gcgccgatcg tgaaccgtgg
      541 agagcttctg caacaggtcg ttcatctgga tggtatcaca ggctggcgtt caggatcgca
      601 tcaggtatac gagaatgtcc cgttctcgcc ggcgatgacg acctccttgg tggcagcggc
      661 ctccacgcga ccgatgatcc gcccctcgat gccgaacgtc cggctgatgc cgatgatctc
      721 ctgtgccctg gcctcgggca cgtacagttc catccgatgt cccatgttga agaccttgta
      781 catctccctc cagtccgtac cgctcatgcg ctgtatggcg gcgaatagcg gaggtaccgg
      841 gaacagagcg tccttgatca cacggagacc ttcgatgaag tgcagcacct tggtctgcgc
      901 ccccccgctg caatgcacca tgccgtggat gtcggcgcgc atggccttga gcacctcacg
      961 gatcaccgga gcgtaggtgc gcgtgggaga aagcacggcc ttgccgaagt cgaggggcgt
     1021 gccgtggagc gggtcggtga ggcctgccct tccagcgtag atcaatgctt ccggcgttcc
     1081 gggatcgaat gtctcggggt agcgctcccc gaccgccttg gtgaacacgt cgtgccgggc
     1141 gctggtgagg ccgttgctgc ccatgcccgc attgtgaccg tcctcgtagg tggcctgacc
     1201 cgaactggca agtcccacga tgatgtcgcc gcccctgatg cgtgcattgt cgatcacatc
     1261 ttcgcggcgc aggcgggcgg tcacggtgct gtccacgatc acggtgcgca ccaggtcgcc
     1321 cacgtcggct gtctcgccac cggtactggt gatgccgatg ccgagcccgc gcatacgctc
     1381 caggaaaagc tccgtgcctt cgatgatcgc tgcgatgacc tcgccgggga tcaggcgctt
     1441 gttgcgcccg atggtgctgc tgagcaggat gttgtccgtg atgcccacgc acagcaggtc
     1501 gtccaggttc atcaccacgg cgtcctgcgc gatcccgtgc cacacgctga ggtcaccggt
     1561 ctccttccag taggcgtacg cgagcgcact cttggtgccc gctccgtcag catgcatcac
     1621 gatacagtgg tccgggcttc ccacgaggct atcgggcacc actttgcaga aggccttggg
     1681 aaaaagtccc ttgtccaatc cggtgatggc ccggtgcaca tcctccttgt cggcggacac
     1741 gcctcgccgg gtataacgat cgttgcctgt catcgggccg tggaaaagaa aagagcatcc
     1801 cgatggctcg ggatgctctc ttgccggttc ttcactactg gttgggcgat ggggtgccgc
     1861 cttccttcgg cacgtaatcg aagctgagca cggtgaagtc cgtgcggcgg ttcttctggt
     1921 gagcggcctc ctgctcctct ttggtcttca tggccttgat ctccttgtcg gtgacgatcg
     1981 gctcggtggc acctttcccg acgggcacca tgcgcaccgg gtcgataccc ttggtcacga
     2041 ggtaattcac gcagctctgc gcgcgcttct ggctcagttc catgttgcca cccttgtatg
     2101 tgcgcgtgtt gcgggagtcg gtgtgagagc gcagttcgat gatgatggtc gggttctcca
     2161 ccaaggtgtt gtacaaggtc tccaacgaat ccttgcccgc ctgggtcagg tcgaacttgt
     2221 ccacctcgta caacacctgc ggcaggggga tccccttgtt ctcgataacc ggttgcaggt
     2281 agtattcctt tgcgaacgtg gtgctttcgt tgaggcccac ggtggtgatc tggtcgttca
     2341 ccacgaggta cttgtccttg cttgcccgga tggtgtagct ggtgttctcc ttgatgtacc
     2401 ggtccttgcc cttctcggtg aagacgaagc cgccattggc gtcggtgagc gcgctgaagc
     2461 tgctgccgtc cgtacccacc acttccacct tcgtgtccgg cagcacgttg ccggtgagct
     2521 tgtcgtagca cgtgccttgg agggcgaact ccaggttggg catcatgaaa cgccagatat
     2581 catcctgccc cttgccgccc gggcggttgc tggtgaagta tcccttctct tcggcaccat
     2641 cgaaaatgat gctgaagtcg tcgccgctgc tgttgatggg gctcttcacg ttctccacat
     2701 ggccccacgt gttgtccccg gtcttttccg ccgcgaagat gtcaagacct cccatgcccg
     2761 gatggccgtc gctgctgaag tacaggttgc cgttctcacg caggaagggg aagtactcgt
     2821 ccttcttggt attgatctcg ggccccagat tggtgggcgt gccggtgggc ttgccgtcct
     2881 tgtcgagccg gatgtaccag agatccttgc ctccctggcc gcccttctgg ttgctggcat
     2941 acaccatgat gccgtcgtcc ttgctcaagg tcggttggcc cagtttgtac tggttggtgt
     3001 cgagtttgcc ggccgctgca tgcagtacga gcatctcggg ttcgctgtag ttgctgccta
     3061 ctttcttggt catccagatc tcgcagccga aggctttctt tttctcgtgc tcgcagcggg
     3121 tgaagtacat caccgtgcgc ttcgcattga atgtggcgct gccttcgtgg gcgggggtgt
     3181 tgatcgtcgg cggcagtttc accggttcgc tccacttgcc cagcttgtcc cggctgctgg
     3241 cgaagagatc caggtaactc tccccggcga taccatccgt ctcagtaccc gtgctggccg
     3301 ggcgactgct ggcgaaaacg acctcgtcgt tctttttcac ggagaacgtc gcgctcatgt
     3361 cgtactgcgg ggagttgagc aatacttcgg gatccacggt gtagcgcgaa gggctgtcct
     3421 tccatgtctg ggcgagttgg caggcggcca ggctcgcgtc ggcacgtttg tccccgggat
     3481 tcttttcctt gtacttgttg tatgcggaga tggcttcggc atacttgccc tgctccttca
     3541 gcgcctcgcc gatccagtag tacgtgatcg gatccgtata ctgggccttg ttggccttct
     3601 cgtaccacac ctgggcgttg ggcgcatcgc cgagtacgcg gtaacactcg gccaccttgt
     3661 agatgaggat ggccttctca ctggccttct tctcggaggt gtacgctttc ttgtacatct
     3721 cgatcgcgtt gaagtactgc ccggccttgt acgcggcgtc cgcttcctcc gcctgcttgt
     3781 tctgggccac cgccaaggtg gtacacagca gactgagggc cgcagtggcc aggatcttcc
     3841 tcatgctcat catgttccgg ttcggtttct ggtttgagag gccgggcgaa tgtaaatgaa
     3901 agtccggatg gacgaacgac cgcaaagtca cccctttcat ggtcaggtcc ccatcagacg
     3961 ggcaccttcc ttggactcaa gctgcggtcg cattcctcag cagcacgttc accgttcgca
     4021 tgttccgcca ccccggtgga tcagggcatt ccaggagttg cgggggcgat cgatcgccga
     4081 acggctcaac gcgtgaacag caattcgcgg agcttgggca ggggccaaag ctcgtcgtcc
     4141 acgttgatct cgagcttgtt cgcgtggtac cggatcttgt ccaggtacgg cttcacctga
     4201 tcacagtagg caatggcctg ctcgcggtga tcctcgatga cgttggcctt tttccgggct
     4261 tcgatcatgt cgtcggtgag cgtcttgagc cgcccgagat gctcactgat ctcttcgatc
     4321 attccgatct gggtggctgc ggctttggcg gctttttcgg gacccaggac gtccttgagg
     4381 ccgcgtacgt tctcgatcaa tcggttctgg tagctgatgg cggcggggat gatgtggttc
     4441 acggccaggt cgccggccac ccggctttcg atctgcagct tgagaacgta gctgtgcaat
     4501 tcgatctcgt ggcgggcctc gagttccact tcgctcagca cccccatctc cgaaaagagc
     4561 ttcttggtct ccgcccgttc ccagacgtca agcgcccgcg gggtgtccgc gatgttgctg
     4621 agcccacgct tcttggcttc ggccttccac tcgtcgccgt atccgttccc ttcgaaccgg
     4681 atggccttgc tctccacgat caggtcgcgg atgacccgca ggatggcctc gtccttcttg
     4741 gtccccttct tgatcatcgc gtccaccgtc ttcttgaacg cgcgcagctg gctggccacg
     4801 atggt
//
LOCUS       DAOQOC010000068         4777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7566_length_4777_cov_4.305591, whole genome shotgun sequence.
ACCESSION   DAOQOC010000068 DAOQOC010000000
VERSION     DAOQOC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7566_length_4777_cov_4.305591"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PL106_01790"
     CDS             <1..616
                     /locus_tag="PL106_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HNA31828.1"
                     /translation="LILGVIALFVYVWAEVLAGDSIPIYAERLGTPLDTAKVLTSLTL
                     SAMLLGYIAGIITIPKYLSQAKALAASCAVALLCVAGIVLSDPGSTATVWIYDFNAFA
                     GRTVELPITVICVALLGLGNALMWPAIWPLAVDGIGGALRTGSAMLIMAILGGALALP
                     VWTAMSPGASVIASQNAYLLLLPFYLYIGWYAMRGHLLRAKAAQ"
     gene            629..1978
                     /gene="mnmE"
                     /locus_tag="PL106_01795"
     CDS             629..1978
                     /gene="mnmE"
                     /locus_tag="PL106_01795"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNA31829.1"
                     /translation="MIGEDTICALSTPAGTGGIALVRLSGPDALRIARVCSDVSGNVL
                     ARMAYLGRFQDAEGGIDEGLLTWFKAPASYTGEDVVELSIHGSPYIAQRLLEALVSAG
                     ARLARPGEFTLRAYLNRKLDLSQAEAVADLIAVQSATAHRLALQQLRGGYSQRIEELR
                     QKLIDFSALIELELDFGEEDVEFARRDDLLAFLDELTGICDDLIRSFRYGNAVKSGVP
                     VAIVGAPNSGKSTLLNALLREDRAIVSDIPGTTRDTVEETITIDGTQFRFIDTAGLRE
                     TGDTIEKLGIERSYRKAEEASIVVLLGDASVLSEGAFRTQAQLLRKRIGEGPALVPVL
                     SKSDLPEARIPAGTLGISARTGHGLDELRQVFVDHVNGLRTDDGGITVTNARHVEALV
                     HAHQALVAARTGISGQLGGELVAADLRRAQHHLGTITGRITPDDLLGSIFGKFCIGK"
     gene            2315..2752
                     /locus_tag="PL106_01800"
     CDS             2315..2752
                     /locus_tag="PL106_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNA31830.1"
                     /translation="MATFEKREKEKKRQQRQEEKKRKKEERKANSAGGGLENMMAYVD
                     EFGRISSTPPDPDKVRKPIDISEIEIGVPRREHVEEPSVRTGHVDFFDSTKGFGFINE
                     DGTHERFFVHVTGLLDEVRDGNKVEFELERGPKGMNAVRVKKV"
     gene            complement(2987..3733)
                     /locus_tag="PL106_01805"
     CDS             complement(2987..3733)
                     /locus_tag="PL106_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31831.1"
                     /translation="MKRILPLAVAVLSAFGAAAQCVPNMNYADSVYGVWPDTVTNFAH
                     GYANTFYSDTLQMLVPTDAGLVDPQGAGFTIDSIALDEVAGLPPGFYVNCVSQTTAPC
                     TYLPSVVGCGLIEGTTDSVGVFDLTINVTVYLLAGFVQQQVSFPGYRIVIEEPNSIAD
                     AVVLKPTDVRAVPNPADAATNIQFGLSRAAQAHIRVYNMVGEKLWDRTVQGKSGANNV
                     RIDASTMGSGIYLYTVEAGSNTFTGRLVVN"
     gene            complement(3760..4134)
                     /locus_tag="PL106_01810"
     CDS             complement(3760..4134)
                     /locus_tag="PL106_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNA31832.1"
                     /translation="MNFTIEDKGKYTLVKTNVDKLDTTCAPELKSELVYLNKTNVRNV
                     IIDLGATRYCDSSGLSALLVANRLCKSVGGTLVVCALQEPVLKLVQISQLESVLSITP
                     TLNEATDLLFMEEIEKDVKKEQ"
     gene            complement(4211..>4777)
                     /locus_tag="PL106_01815"
     CDS             complement(4211..>4777)
                     /locus_tag="PL106_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNA31833.1"
                     /translation="IVNAGDGTHEHPTQALLDAYSIREKFGRIKGLRILIVGDIAHSR
                     VALSDIHCFKKLGAEVMLCGPTTLMPRDVRSLGVRVEHDLDKALRWCDVANMLRIQLE
                     RQGGDGIANFPSLREYSMLFGLDEERYSRAGKPITIMHPGPINRGVEITSGIADHADS
                     IILDQVENGVAVRMAVLYLLAARIPRPV"
BASE COUNT          934 a         1430 c         1521 g          892 t
ORIGIN      
        1 actgatcctc ggcgtcatcg ccctgttcgt gtacgtgtgg gccgaggtgc tcgccggcga
       61 cagcatcccc atctatgcgg aacggctcgg caccccgctc gatacggcca aggtgctcac
      121 cagcttgacg ctttcggcca tgctgctggg ttacatcgcg gggatcatca ccattcccaa
      181 gtatttgtcc caggcgaagg ccctcgcggc ctcgtgcgcg gtagcgctgt tatgcgtggc
      241 ggggatcgtg ctgagcgacc cgggcagcac ggccacggta tggatctacg acttcaacgc
      301 gttcgccggc cgcacggtgg agttgcccat caccgtgatc tgcgtggcgc tgctcgggtt
      361 gggcaacgcg ttgatgtggc cggcgatctg gcccctggcc gtggacggca tcggcggcgc
      421 attacggacc ggatcggcca tgctgatcat ggccatcctg ggcggcgccc tcgccctgcc
      481 ggtctggacc gccatgtcgc ccggggcctc ggtgatcgca tcacagaacg cctacctgct
      541 gctcctgccg ttctacctgt acatcggctg gtacgctatg cgcgggcacc tgctacgggc
      601 gaaagcggcg cagtgaccaa cttgcaccgt gatcggagag gacaccattt gcgccctgag
      661 cacacccgct ggtaccgggg gcatcgcctt ggtgcgtctc agcgggcctg atgcgttgcg
      721 gatagcccgg gtctgctcgg acgtgagcgg caacgtgctt gcgcgcatgg cgtacctggg
      781 tcgattccag gatgccgaag gcgggatcga tgaaggcttg ctcacctggt tcaaggcccc
      841 ggcctcatac accggcgagg atgtggtgga actgagcata catggttcgc cctacatcgc
      901 tcagcgcctg ctggaagcct tggtgagcgc gggtgcacgg ctggctcggc ccggcgaatt
      961 caccctacgt gcctacctca atcggaagct ggacctgagc caggccgagg ccgtggccga
     1021 cctgatcgcg gtacagagcg ccaccgcgca ccggttggca ctgcagcaac tgcgcggcgg
     1081 atacagtcag cgcatcgagg aactgcgcca gaaactcatc gacttcagcg cgctgatcga
     1141 attggagttg gacttcggtg aggaggatgt ggaattcgcc cgacgggacg acctgctggc
     1201 gttcctggac gagttgaccg gtatctgcga cgacctgatc cgcagtttca ggtacgggaa
     1261 tgccgtgaaa agcggtgtgc ccgtggccat cgtgggcgcg cccaacagcg gcaaaagcac
     1321 cttgctgaac gcgttgctgc gcgaggatcg tgccatcgtg agcgacatcc cgggaaccac
     1381 gcgggacacg gtggaggaga ccatcaccat cgatggcacc caattccgct tcatcgacac
     1441 cgccggcctg cgcgaaacag gggacaccat tgagaaactc ggtatcgagc gcagttaccg
     1501 gaaggcggag gaagcgagca tcgtggtcct cctgggcgac gcgagtgtcc tgagcgaggg
     1561 agccttccga acgcaggccc aactgctccg gaagcgcatc ggcgaaggtc ctgcactggt
     1621 gcccgtgctc agcaagtccg acctgccgga agccaggatc ccggcgggaa cattgggcat
     1681 cagtgcacgc accggtcacg ggctggacga gttgcggcag gtattcgtgg accatgtgaa
     1741 cggtttgcgc accgacgatg gcggcatcac cgtgaccaat gcacggcatg tggaagcgct
     1801 cgtgcatgcg catcaggccc tggtggccgc acgcacgggc atcagtgggc aacttggagg
     1861 cgaactcgtg gcggccgacc tgcgacgggc ccaacatcac ctgggcacga tcaccggcag
     1921 gatcacaccg gacgacctcc tgggaagcat attcgggaag ttctgcattg gaaaataggt
     1981 tctgaatact ggtatcgtat cagtatgtgc cgcaggaccc gggctccggg gaagacactc
     2041 ggacatggat cggtggtgat gcatgatcga ccagtcgtga tcggctccca tggttggact
     2101 cccacccgct cgtgatcggg ccaccggacc ggttccgaac aagggccgga tcaagcatcc
     2161 gtggatcggg cctggacagg atgggagaga acagaccggc agggcctatc ttcgcacgta
     2221 caaacatcgc cccaaggccg agaagcgttt acccgttccc gcatgagcgg taccgtgggt
     2281 gaggccgacc ggcacttgag cccaacaccg caacatggcc acattcgaga agcgcgaaaa
     2341 ggagaagaag cgtcagcaac gacaggagga gaagaagcgc aagaaggagg agcgcaaggc
     2401 caacagcgct ggtggcgggc tggagaacat gatggcgtac gtggacgagt tcggccggat
     2461 cagcagcacg ccgcccgacc cggacaaggt gcgcaaaccc atagacattt cggagatcga
     2521 gatcggtgta ccacgacgtg agcatgttga agaaccgtcg gtgcgcacgg gccatgtgga
     2581 cttttttgac agtaccaagg gcttcggctt catcaacgag gatggcacgc acgaacgctt
     2641 tttcgtacac gtcacgggcc tgctcgatga ggtgcgcgac ggcaacaagg tggagttcga
     2701 gcttgagcgt ggccccaagg gcatgaacgc ggtgcgggtg aagaaggtgt gatccggcgc
     2761 tgcgacaacg gagcgcgagg ggtgtacttc gtccattccc tggtgaacgg tacgccgcac
     2821 attgtacggc tccacttcag cacatcgtga cgatcgcacg gtgctacggc ctgtccagtg
     2881 ccaccatcgg gatcaggcag gacgcgggcc acatgcatgg accctccaac aaaaaggggc
     2941 cgtccacgga tggaacggcc cctttcacga acgttagtcc gccagatcag ttcaccacga
     3001 gccgaccggt aaaggtgttg gagcccgcct ccaccgtgta gagatagata ccgctgccca
     3061 tggtgctggc gtcgatccga acattattgg ccccggactt cccctggacc gtacgatccc
     3121 aaagcttctc accgaccatg ttgtacacac ggatatgcgc ctgggccgca cgcgacaggc
     3181 cgaactggat gttggtggcg gcatcggcag gattgggcac tgcgcgcaca tcggtcggct
     3241 tcaggaccac cgcatcggcg atgctgttgg gttcctcgat cacgatccgg tagcccggaa
     3301 aactcacctg ttgctgaacg aaacccgcga ggaggtatac ggtaacgttg atggtaaggt
     3361 cgaacacgcc gaccgaatcg gtggtgcctt cgatcaggcc gcagcccacc acactgggca
     3421 gatacgtaca tggggcggtg gtctgtgaaa cacaattcac atagaagccc gggggcaggc
     3481 ccgctacttc gtccaatgcg atggaatcga tcgtgaagcc ggcgccctga ggatccacca
     3541 gaccggcatc cgtgggcacg agcatctgca aggtatcgct gtagaaggtg ttggcgtaac
     3601 cgtgggcgaa gttcgtcacg gtatcgggcc atacgccgta cacgctgtca gcgtagttca
     3661 tgttgggcac gcactgggcc gcagccccga aggcggacag tacggcgact gccaggggta
     3721 gtattctttt catgttgtgg tcgtaccgcg cgcagcggtt cattgttcct ttttcacatc
     3781 cttctcgatc tcctccatga agagcagatc ggtggcctcg ttcaacgtgg gtgtgatgga
     3841 cagcacgctc tcgagctgac tgatctgcac cagcttgagc accggctcct ggagcgcgca
     3901 caccaccagg gtgccaccaa cgctcttgca gaggcggttg gccaccagca gggcgctgag
     3961 tccgctgctg tcgcagtagc gcgtggcgcc taggtcgatg atcacgttgc gcacattcgt
     4021 cttgttcagg tagacgagtt cgcttttcaa ctcaggggca caggtggtat ccaacttgtc
     4081 cacgttggtt tttaccagcg tgtacttgcc tttgtcctcg atggtgaaat tcatggtcat
     4141 gtccttctgg tggaccttgc ggccaaatat agcgatggcg tgcggtggcg cgttcctccg
     4201 caaagtcgcg tcagacgggc ctcgggatac gggcggccag caagtagagc accgccatcc
     4261 ttacggcgac gccgttttcc acctgatcga ggatgatgct gtcggcatgg tcggcgatac
     4321 cggaagttat ctccacacct ctgttgatag gccccggatg catgatggtg atcggtttgc
     4381 ccgcccggga gtatcgttcc tcgtccagac cgaagagcat gctgtactcc cgcaggctcg
     4441 ggaaattggc gataccatcg cccccctgcc gttccaattg gatgcgaagc atgttggcca
     4501 catcacacca ccggagtgcc ttgtccaggt cgtgttccac gcgcacaccc agactccgca
     4561 catcgcgcgg catgagggtg gtgggaccgc aaagcatcac ttcggccccc agtttcttga
     4621 aacagtggat gtcgctgagc gccaccctac tgtgggcgat atcccccacg atgagtatgc
     4681 gcaagccctt gatgcgaccg aatttctcgc ggatgctgta cgcgtccagc aatgcttgtg
     4741 tagggtgctc atgcgtaccg tcgcccgcat tcacgat
//
LOCUS       DAOQOC010000069         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7652_length_4746_cov_3.283948, whole genome shotgun sequence.
ACCESSION   DAOQOC010000069 DAOQOC010000000
VERSION     DAOQOC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7652_length_4746_cov_3.283948"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PL106_01820"
     CDS             <1..241
                     /locus_tag="PL106_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31834.1"
                     /translation="QALADGDHAQAHKLLTRYMRDLEDHISAENDELFVLAENLLGKK
                     EREDVYFRFMDIDRQLGEDRKKELEELLAAVKVEG"
     gene            261..599
                     /locus_tag="PL106_01825"
     CDS             261..599
                     /locus_tag="PL106_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31835.1"
                     /translation="MNASGKLAWIKVVHTVIWAVLSAVIFFLLYAVITDRLDHWFWWG
                     LGLVGIEVLTLLVFGMSCPLTVLARRLSSSQRANFDIFLPEWLARYNKAICSAILLVV
                     LAGMAWRLLK"
     gene            758..1795
                     /locus_tag="PL106_01830"
     CDS             758..1795
                     /locus_tag="PL106_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNA31836.1"
                     /translation="MIHKFSPIEKLLLSLDVIPHPFTDASSSVGLGFALGSAVKLGIT
                     DQLTEAFKEVKQIAQGAKVSEVGAELILDCLDALGYVKKQGTRYAFTKRGTKNLSAQS
                     PENFRHFILFCDYLYKGYLDLDGTIRAGKRQGTNMLEQMNDREWELFSRAMIDIARTN
                     HKEVAKKIPVSASQTRMLDLGGSHGLYSIELCRRHPQLKATIVDLPPVKKYADECIAQ
                     HGAAASVSFHPADFMKDDLPKGVDIILAFNIIHGLNPQENGTLARKVFDALNAGGIYV
                     ILDQIKGIGGSSQLSKATTSYMALNLLHQAGGKTYAKSEIDEFTRKAGFKGTELKKLN
                     APGFGVVICRK"
     gene            1838..2743
                     /locus_tag="PL106_01835"
     CDS             1838..2743
                     /locus_tag="PL106_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA31837.1"
                     /translation="MKIKQRSFFKDEKQDLLFLDNWIKKLERLNGRAYERIEIDTALG
                     KTQVWGINTKENSETLVIFPGARTTSLFWDFDKGLDNLGLKLRIFLVETNGLPNFSNG
                     ATPDIRTLDYGHWANEVLEKLKIERAFVAGASFGGLICMKLGITDPDKLKAAFLLNPG
                     CLQPFSMKWSNLYFNLLPIIKPTEKNVLKFLDKAVFSKPDHQLSQGSEALIIEYEVFA
                     LKRYKDNTQKPYYMGKQLADVEVDTYLLEGNKDLLFPYQRSIDNAKKYIKTLKDVKVF
                     DNVGHGIETYDKAMNYIGATIKNYR"
     gene            3197..3892
                     /locus_tag="PL106_01840"
     CDS             3197..3892
                     /locus_tag="PL106_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNA31838.1"
                     /translation="MEKNVEQYHLFRDGRIKMPEHLAPRREVRGRLFGNRLMEALTRS
                     RIEPPIVMHLLISGALFTYGIVSLGIVWWTAVIVAFSGCLFWSFAEYNVHRFLYHTET
                     NSRPLYRIQHNAHGIHHQHPVDPRRLAMPPVPGLLFSGLFFLLFWLVSPTYVFVFFPG
                     FMLGYLTYITMHYAQHRYKTPRYGPWKKLWQHHFVHHYVDPYSCYGVSTRLWDHVFGT
                     TAKVKAGPAVPGS"
     gene            3926..>4746
                     /locus_tag="PL106_01845"
     CDS             3926..>4746
                     /locus_tag="PL106_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31839.1"
                     /translation="MKKFILLGTALLASFTSGAQRVEWVDYAYCTTQWDGGTGIATDD
                     AGHVYVLGSISAELIIQIDTFTNQYGTDDILLMKYDPQGDLVWGKVIGSPILDWSSEL
                     AVDGYGHLYAECTVQGTTMMSDTTYDAPFDHQVIQFDTAGNFLRYFSHLREIIADAQG
                     SAVYLAYANIVEKRDTALNVLWNRTASNDHLYFSNVGPQGGRASMMVGNNGHLVLAGY
                     ENSVGGSTLFDTLLLKFSAAGYCDELCVISMDTSGSALWVRTLDSSSTYQELTPKV"
BASE COUNT         1097 a         1346 c         1326 g          977 t
ORIGIN      
        1 acaggccctg gccgacgggg accacgccca ggcgcacaag ttgctcaccc gctacatgcg
       61 cgacctggag gaccacatct ccgccgagaa cgacgagctc ttcgtgctgg ccgagaacct
      121 gctgggcaaa aaggagcgcg aggacgtgta tttccgcttc atggacatcg accgccagtt
      181 gggcgaagac cgcaagaagg aattggaaga gctgctggcg gcggtgaagg tggaggggtg
      241 agccacagct ccttcgggcc atgaacgcat ccggcaagct ggcatggatc aaggtggtgc
      301 ataccgtgat ctgggccgtg ctcagcgcgg tgatcttctt cctgctgtac gcggtgatca
      361 ccgaccgcct cgatcattgg ttctggtggg gcctgggctt ggtggggatc gaggtgctca
      421 cgttgttggt cttcggcatg agttgtccgc tcaccgtgct cgcgcggcgg ctctccagct
      481 cgcagcgggc caacttcgac atcttcctgc ccgagtggct ggcccgctac aacaaggcca
      541 tttgctccgc gatcctgttg gtggtgctgg cgggcatggc gtggcggctg ttgaagtagc
      601 agggcgcaat gccggtcggt ggcgatccga cttcctcggg cgccgtcatt caccggtatc
      661 atcccgctcc ggggcattcc gatgcgaacc gcccaggatg gaggatcgaa cagggccgca
      721 acttcatcct actttcacgt tccgaactcg caacgccatg atccacaaat tctccccgat
      781 cgagaagttg ctgctctccc tcgacgtcat tccgcatccg ttcaccgacg cctcgtcctc
      841 ggtgggattg ggcttcgcct tgggaagcgc ggtgaagctc ggcatcaccg accaattgac
      901 cgaagcgttc aaggaggtga agcagatcgc ccagggcgcc aaggtcagcg aggtgggggc
      961 cgaactgatc ctggactgcc tggatgccct ggggtacgtg aagaagcaag ggacgcgtta
     1021 tgccttcacg aaacgcggga ccaagaacct ctccgcgcag tcgccggaga acttccggca
     1081 cttcatcctc ttctgcgact atctgtacaa gggctatctc gacctggacg gaacgatccg
     1141 cgcgggcaag aggcagggta cgaacatgct ggagcagatg aacgaccggg aatgggagct
     1201 cttctccagg gccatgatcg acatcgcgcg gaccaaccac aaggaggtgg ccaaaaagat
     1261 cccggtctcc gcgtcgcaga cccgcatgct cgacctcggc ggatcgcatg gcctgtacag
     1321 catcgaactc tgcaggcgcc acccgcaact gaaggccacc atcgtggact tgccgccggt
     1381 gaagaagtac gccgacgagt gcatcgcgca gcatggcgcg gcggcgagcg tgtccttcca
     1441 tcctgcggac ttcatgaagg atgacctgcc caagggcgtg gacatcatcc ttgccttcaa
     1501 catcatccac ggcctgaacc cgcaggagaa cgggaccctg gcgaggaagg tgttcgacgc
     1561 actgaacgcg ggcggcatct acgtgatcct cgaccagatc aaggggatcg gcggcagttc
     1621 gcagctatcg aaagccacga cctcttacat ggccctgaac ctcctgcatc aggccggtgg
     1681 aaaaacatac gcgaagagcg agatcgacga gttcacgcga aaggcggggt tcaaggggac
     1741 ggaactgaag aagctcaacg ctcccggttt cggcgtggtg atctgccgca aataggccgt
     1801 tagcggtaat tttgccgaac catagaacaa agagaggatg aagatcaaac aacgctcgtt
     1861 tttcaaagat gaaaagcagg atcttttatt tcttgataat tggataaaga agttggagag
     1921 attgaacggc agagcgtatg agcgaattga gatcgacacc gctttgggca aaacccaggt
     1981 gtggggaata aacaccaagg agaattcaga gacgttagtg atattcccgg gagcaaggac
     2041 gacttccttg ttctgggatt ttgataaagg acttgataac ttgggtctca aactcagaat
     2101 tttcctggtt gaaacgaacg gactgcccaa cttcagcaat ggcgctacgc ctgacattag
     2161 aacattggat tatggacatt gggcaaatga agtcctggag aaactgaaga tcgaaagagc
     2221 atttgtagcg ggtgcatcct ttggtggttt gatctgtatg aaattgggaa ttacagatcc
     2281 tgacaaactg aaggcggctt ttctcttgaa tcctggatgt ttgcagccgt tctcaatgaa
     2341 atggagcaat ctgtacttca atctgttacc aataataaaa cccacggaaa aaaatgttct
     2401 caaatttttg gacaaggccg ttttttcaaa acccgaccat caattatcac aaggatcaga
     2461 agcattgatc atagaatatg aggtatttgc tctgaaacgc tacaaggaca acacacaaaa
     2521 accatactat atgggcaaac agttggctga cgttgaagtt gacacatacc ttcttgaagg
     2581 caataaagat ctgttattcc cgtatcaaag atcaattgac aatgcaaaaa aatatatcaa
     2641 aacattaaaa gacgtaaaag tttttgataa cgttggacat ggaatagaaa cctatgacaa
     2701 agcaatgaac tatattggag caaccattaa aaactaccgc taatatcgat cttgcgtcat
     2761 ggaggcggac gtgcttcgtg gaaatagttg agttggaaca aacatttgcg cctcgaatga
     2821 acgttggcgc tattgagtcg gcacgatctc gttttgagac acccttgggc cagttgatcc
     2881 gagaacagca ggaactccgt tagctgcgat caaaacccaa tgatgccgac cctataagga
     2941 tccccccacc aggcaaagcc ccggaacggt ttgcatggag cagtaggcta ccccggcccc
     3001 tctcactctt gccacgttcc gaggaaagtg tgtgcatgcg gaggagttct ttagacttga
     3061 tctgaattgc ggtttcctga ttaatatcag ggatggcccg gcactacgca atatagtttt
     3121 caatcaacct atcctgacga tcggctgatc atccggatgt tcaacgaacc agcggcaccg
     3181 cacatgctgg atcgcgatgg aaaagaacgt tgaacaatat catctattcc gcgacgggcg
     3241 gatcaagatg cccgagcacc tggccccgcg cagggaagtc cggggacgcc tgttcggcaa
     3301 ccggttgatg gaggccctca cgcgcagccg catcgagccg ccgatcgtga tgcatctgct
     3361 catcagtgga gcgctgttca cctacggcat cgtctcgctc ggcatcgttt ggtggacggc
     3421 ggtcatcgtc gctttctcgg gctgcctctt ctggtcgttc gccgagtaca acgtccaccg
     3481 cttcctgtac cacaccgaga ccaactcgag gccactgtac aggatccagc acaatgcgca
     3541 cggcatccac caccagcatc cggtggatcc gcggcggctc gcgatgcctc ccgttccggg
     3601 cctgctcttc tcgggcctgt tcttcctgct cttctggctg gtgagcccca cctacgtctt
     3661 cgtgttcttc cccggcttca tgctgggcta cctcacctac atcaccatgc actacgccca
     3721 gcaccggtac aagacgccgc gctacggccc ctggaagaag ctctggcagc accacttcgt
     3781 gcatcactac gtggacccct attcctgcta tggcgtttcc acgcggttgt gggaccatgt
     3841 tttcgggacc accgcgaagg tgaaggccgg gcctgccgtg cccggatcgt aatggcaacc
     3901 agtgatcgct tcaacccaat accccatgaa aaaattcatc ctcctcggca ccgcccttct
     3961 cgcctcgttc acctccggtg cccagcgcgt ggaatgggtg gactatgcct actgcaccac
     4021 acaatgggac gggggcacgg gcatcgccac cgacgatgcc ggccatgtgt atgtgctggg
     4081 cagcatctcg gccgagctca tcatccagat cgatacgttc accaaccagt atggcacgga
     4141 cgatatcctg ctgatgaagt acgacccgca aggggacctt gtgtggggca aggtgatcgg
     4201 cagccccata ctcgattggt cctctgaact cgccgtcgat ggatatgggc atctctacgc
     4261 cgagtgtacc gtgcagggca ccacgatgat gagcgacacc acgtatgacg ccccgttcga
     4321 ccaccaggtc atccagttcg acacggcggg gaacttcctg cgctacttca gccatttgcg
     4381 cgagatcatc gccgatgcgc agggaagcgc cgtctatctg gcgtatgcca acattgttga
     4441 aaagcgcgac accgccctca acgtgctctg gaaccgcacc gcctccaacg accacctcta
     4501 cttcagcaac gtcggtccgc agggtgggcg cgcgagcatg atggtgggca acaacggcca
     4561 cctcgtactg gcgggatacg agaactccgt gggaggaagc accctgttcg acacgctctt
     4621 gctgaagttt tccgctgcgg gatactgcga tgagctctgc gtgatcagca tggacaccag
     4681 cggatcggcc ctgtgggtgc gaacgctcga cagcagttcc acctaccagg agctgacccc
     4741 gaaggt
//
LOCUS       DAOQOC010000070         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7700_length_4724_cov_3.705719, whole genome shotgun sequence.
ACCESSION   DAOQOC010000070 DAOQOC010000000
VERSION     DAOQOC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7700_length_4724_cov_3.705719"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..651)
                     /locus_tag="PL106_01850"
     CDS             complement(415..651)
                     /locus_tag="PL106_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31840.1"
                     /translation="MSEVELKKRLISRIQRSRNPSLLREAFRLMGTDAADLEPYKLTK
                     EQQASVNRGKKDVKAERTLTERAANKAVDEWLGK"
     gene            complement(773..1144)
                     /locus_tag="PL106_01855"
     CDS             complement(773..1144)
                     /locus_tag="PL106_01855"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HNA31841.1"
                     /translation="MMKRISLILVTSFIASFASAQVAEGLAKIPDATKPLLNVEATCG
                     ECKLKMHGEGCDLAVRLPEGSYYVDGAGISSFGHPHDENGFCLAIRRAEVQGEVVDGR
                     FKASYFKLLPPGTEAPKAKEK"
     gene            1244..1855
                     /locus_tag="PL106_01860"
     CDS             1244..1855
                     /locus_tag="PL106_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNA31842.1"
                     /translation="MIHLLRPTQCIVIEPRSCYGAARRTDGQGSYVGYLSKDAIFSAY
                     RKILVEQGSGLAVPVHPRRTLGNVILDGYQAWPETAWVVLEQCPCNPCGRSSHAIRSN
                     MNYTENFEGIKLDVQAVDHEVEAGVQEQIRKMLSRLGRHFQGITHADVYLEDKAAKSN
                     DPKQVSVRLGIPGNDPFASERGDNFMALLANVEEKLRRQLEKR"
     gene            1870..2676
                     /locus_tag="PL106_01865"
     CDS             1870..2676
                     /locus_tag="PL106_01865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA31843.1"
                     /translation="MAKTKHGKAKAPLSDLLSLVGRKALVTGAASGIGRAMALRLAEA
                     GAQLVLMDRDEEKLGNLREQMPTAELHALDISNKSAIDRFWEGRTARDIDILVNNAGI
                     YPFKPFLELDAAYLARVMDTNLYSVLWMCQGFIRNRIKQGGVVVNIGSVEAIMPFKKD
                     VVHYSMSKVGVITLTRDLAREFGDKGFRVNAIIPGGILTEGTKSAAKQAIITLDTRLM
                     SDSYNFYQRLPIGRFGDPDEVARMAVVLSSNLSSYVHGALLPVDGGFLSA"
     gene            complement(2716..3231)
                     /locus_tag="PL106_01870"
     CDS             complement(2716..3231)
                     /locus_tag="PL106_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31844.1"
                     /translation="MNRRLHDFFTRDHRRLEALLEQAFADPGAIDHEAYGQFRAGLLR
                     HIGMEEKILFVAAQEANAGAPLPVQAKLRLDHGALTSLMVIPPSVALTRVIRHVLDLH
                     DDIEEKPGGMYDACEQLTEHRTDVLLERLAHVPETPVHPPNPSHKAIGAARRAMARAG
                     FDYDLLGGPGE"
     gene            complement(3328..4077)
                     /locus_tag="PL106_01875"
     CDS             complement(3328..4077)
                     /locus_tag="PL106_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNA31845.1"
                     /translation="MNNRIHPPQELDVRVWVPIERHRNLLKLFKELPVGESFIFINDH
                     DPLPLYYEFRSIHGDVVGWEYLQRGGQDWKVMVTRLEASVGREFTDISTLMDLRKVAD
                     EDWKHVVFHRYGMMEEGHVMELIAAQEPKHVRMLFEERFAGKHSWTYKKQEPGEWVAH
                     IRKQGKQTAPAAGAVVSTFDVRPFHPAKRHEMVFDSFDALKPGEAFIFTNDHDPKPLY
                     YQIEAESKIPFTWEYLQAGPEVWEVRVTKTN"
     gene            complement(4143..>4724)
                     /locus_tag="PL106_01880"
     CDS             complement(4143..>4724)
                     /locus_tag="PL106_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA31846.1"
                     /translation="GTTWDMAVINDTIMLGDTEIWTWVNHSNMAHPMTVHGGSFYVLD
                     RNGDLPPEWERGPKSVVHVDVDDTVRTIMKFATYTTGDWPLMYHCHNLMHIEEKMWQF
                     VIVDPSTDVVEQASTEEVRVFPVPASSMVTYHAPFPVRRVRVIDPSGREVINASGSMT
                     TDGSIDMASLVPGMYIAWLEGPGGPARVRVVRE"
BASE COUNT          907 a         1424 c         1420 g          973 t
ORIGIN      
        1 gtaaggttca acccgcgcta gccgcaccgg gccctatcgc cgcgctcatg gcaacgcaac
       61 aagtgcccgc tttcagtcgg tcgtgtgggg cacgtccgac ggtctgttac ctgctttggt
      121 cttgtgctgg ggatgttagg ccggtctggt catcgggtct cgcctgccgt gccataccga
      181 aagcacagta aacctccgtt gtgctgaaag agtagaagag catgtgttcg cccacactct
      241 tcatgcgcac ttcaggatct gaagtagcgc gcccgatctt cggatgctcc gcgaccagtc
      301 gcaacgatgc gcggaacatg ccatccagtt tttcgctgta ccgagttgaa ccgttgcggt
      361 cggcccagta ctttaggatc tccagtcgtt cgagctgagc gttggccgac cagactattt
      421 gccgagccat tcgtccacgg ccttgttcgc cgcacgctcg gtgagggtgc gctctgcttt
      481 cacatctttc ttgccgcggt tcaccgaggc ctgctgctcc ttggtaagct tataaggctc
      541 aagatccgca gcgtcagtac ccataagccg gaacgcctcg cgcagcagtg agggattacg
      601 cgaacgctgt atgcggctga tcaaacgctt tttcagttcc acttcggaca tggccttcgg
      661 atttgtagag ctaaggtacg cttggggccc gcactgtgat gtcctaacgc acgcaccagc
      721 cgcaccgtgc cctatcgacg ctggcccgac ccggttccgt tgagtgttcc ccctacttct
      781 cctttgcctt cggcgcctcg gttcccggtg gcagcagctt gaagtaggat gctttgaaac
      841 gcccatccac cacctcgccc tgcacctcgg cccggcggat ggccaggcag aagccgttct
      901 catcgtgcgg atgtccgaag gagctgatgc ccgcaccgtc cacgtaatag gagccttcgg
      961 gcaggcgcac cgccaggtcg cagccttcgc cgtgcatttt cagtttgcat tcgccgcagg
     1021 tggcttccac gttcagcagg ggcttggtgg catcggggat cttggccaac ccctccgcta
     1081 cctgagctga agcgaaggag gctatgaacg acgttacaag gatgaggctg atccgtttca
     1141 tcatgggttc gggtttcttc ggcgaaaggt acggcacagg ggaaacctct tgaggcagcg
     1201 actcacgcat caagcccacg accgcctggc gcaacgggct gggttgatcc atctcctgcg
     1261 gcccacccag tgcatcgtga tcgaaccccg ctcgtgctat ggcgcggcgc gccgcaccga
     1321 tggccaaggc agctatgtgg gctacctcag caaggatgcc atcttcagtg cctaccggaa
     1381 gatactggtg gagcagggga gcggcttagc ggttcctgtt cacccaagga ggactttggg
     1441 caatgtgatc cttgatggat atcaggcgtg gccggaaacc gcatgggtag ttttggaaca
     1501 atgcccatgc aacccatgcg gcaggtcatc acacgccata cgaagcaaca tgaactacac
     1561 ggagaatttt gaagggatca agctggatgt gcaagccgtg gaccatgaag tggaagccgg
     1621 ggtgcaggag caaatacgca agatgctttc gcgcctgggc cgccacttcc aaggcattac
     1681 gcatgccgat gtgtacttgg aggacaaggc ggcaaaatcc aatgacccca aacaggtaag
     1741 cgttcgcttg ggcattccgg gcaacgaccc tttcgcctca gagcgtggcg acaatttcat
     1801 ggccttgctg gcgaacgtgg aggagaaact gaggaggcag ttggaaaagc gttgacacgc
     1861 ttcaggaaca tggcaaagac caagcacggc aaggccaagg ccccgctcag cgacctgctc
     1921 tccttagtgg gccggaaagc attggtgacc ggcgccgcaa gcggcatcgg tcgtgccatg
     1981 gccctgcgat tggccgaggc gggcgcccaa ttggtgctga tggaccgtga tgaggagaag
     2041 ctcggcaact tgagggagca gatgcccact gccgagctgc acgcgctcga catcagcaac
     2101 aaatccgcga tcgaccgttt ttgggagggc cgcaccgcac gggacatcga cattttggtg
     2161 aacaacgcag gcatctatcc cttcaagccg ttcttggagc tggacgcggc ctatttggcc
     2221 cgcgtgatgg acaccaacct ctattccgtg ctgtggatgt gccagggctt tattcggaac
     2281 cggatcaagc agggcggtgt ggtggtgaac atcggttcgg tggaagcgat catgcccttc
     2341 aaaaaggatg tggtgcacta ttccatgagc aaggtgggag tgatcaccct gacgcgcgat
     2401 ctggcacgtg aattcggcga caagggcttc cgggtgaatg ccatcattcc cggcggcatc
     2461 ctgaccgaag gcacaaagtc cgcggccaag caagccatca tcaccttgga tacccggctg
     2521 atgtcggact cgtacaattt ctaccagcgc ctgcccattg gccgcttcgg cgacccggac
     2581 gaagtggcac gcatggccgt ggtgctttca tccaacctgt ccagttacgt tcacggcgca
     2641 ttgctgcccg tggacggagg gtttctttcg gcctaggcag atgcgcctac agtcagccta
     2701 ttttatcaac caggcttact cccccggccc acccaacaag tcgtaatcaa accccgcgcg
     2761 ggccatggcg cggcgtgcgg caccaatggc cttgtgcgat gggttgggcg gatgcaccgg
     2821 cgtttccggt acgtgggcca agcgttccag caacacatcc gtccgatgtt cggtgagttg
     2881 ctcgcacgca tcatacatgc cgcccggctt ctcctcgatg tcgtcatgca ggtccaacac
     2941 atggcggatc acacgggtga gggccacact gggcggaatg accatgaggg aggtgagtgc
     3001 gccgtgatcc aagcgcagct tggcctgcac ggggagcggc gctccagcgt tggcctcctg
     3061 agccgctaca aagaggatct tttcctccat gccgatgtgc cgcagcaacc cggcgcggaa
     3121 ttggccgtac gcctcgtgat cgatcgcgcc aggatccgca aaggcttgtt ccaacagggc
     3181 ctccaggcgg cggtggtccc gggtgaaaaa gtcgtgcagg cggcggttca tcgatggaag
     3241 agcaagagcg tacgcgggtg cgaggggtcc tgaatgcgtg caatactacc ctgtcagccg
     3301 cacactcttc aactcagttc gctgacatca attcgtcttc gtcacccgca cctcccacac
     3361 ctcgggcccg gcctgaagat actcccaggt gaatggtatt ttgctttccg cctcgatctg
     3421 gtagtagagc ggcttggggt cgtggtcgtt ggtgaagatg aaggcctcgc cgggcttcaa
     3481 cgcatcgaag gagtcgaaga ccatctcatg gcgcttggcc gggtggaacg ggcgcacatc
     3541 gaaggtgctt accaccgcgc ctgccgccgg agcggtttgc ttcccctgtt tgcggatgtg
     3601 cgccacccac tccccgggtt cctgcttctt gtaggtccag ctgtgctttc cggcgaagcg
     3661 ttcctcgaag agcatgcgca catgcttggg ctcttgcgcc gcgatcagct ccatcacatg
     3721 gccttcctcc atcatcccat agcggtggaa caccacgtgc ttccagtcct cgtcggccac
     3781 tttgcgcagg tccatcagcg tggagatatc cgtgaactcg cggcccaccg aggcttccag
     3841 gcgggtgacc atcaccttcc agtcctgacc gccgcgctgc aggtactccc agcccaccac
     3901 gtcgccgtgg atggagcgga actcatagta gagtggcaac ggatcgtggt cgttgatgaa
     3961 gatgaagctc tcgccgacgg gcaactcctt gaacagcttg agcaggttgc ggtgccgctc
     4021 gatcggtacc caaacgcgga catcaagttc ttggggcgga tggatccggt tgttcatgtc
     4081 taccgtgtct ggatcacggg gcgggtcgat cggatggctc gtctggttgc ggatgatcgg
     4141 gtctactcgc gtacgacgcg cacccgtgcc ggtcctcccg gtccctccaa ccacgcgatg
     4201 tacatgccag gtaccaacga cgccatatca atgctcccat cggtggtcat ggaacccgat
     4261 gcattgatca cttcacgtcc cgagggatcg atcacccgca cccgcctgac cgggaagggg
     4321 gcgtggtagg tgaccatgga ggatgccggc accgggaaca cgcgcacctc ttcggtggat
     4381 gcctgctcaa ccacatccgt gctcggatcc acgatgacga actgccacat cttctcctcg
     4441 atgtgcatca ggttgtggca atggtacatc aggggccaat cgccggtggt gtatgtggcg
     4501 aacttcatga tggtgcgcac ggtgtcgtcc acgtccacat gcaccacgga tttcggtccg
     4561 cgctcccatt cgggcggaag gtccccattg cggtccagca cgtagaacga gccaccatgc
     4621 acggtcatgg ggtgggccat gttggaatgg ttcacccagg tccagatctc ggtgtctccc
     4681 agcatgatgg tatcgttgat cacggccatg tcccaagtgg tgcc
//
LOCUS       DAOQOC010000071         4686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7825_length_4686_cov_3.846469, whole genome shotgun sequence.
ACCESSION   DAOQOC010000071 DAOQOC010000000
VERSION     DAOQOC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_4686_cov_3.846469"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PL106_01885"
     CDS             complement(<1..690)
                     /locus_tag="PL106_01885"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31847.1"
                     /translation="MAASPALLQFSINGSPLGNIFAAPGGTCNWQQFFQLWNSGSNSS
                     ATICIVNQNTTLGGNDFALDDILFAPTCMVKDTVTVHVVSVTAAASPAISFIPCDGAS
                     ITLSGAGSSTGANITYNWDSPNGNIVSGQNTLSPVVNAAGTYTLTVTYDNGLVTCTKT
                     ATVTVAESPNQLAAWINPPTPLGCGSGTSTLIGNSSQPAFSTYEWTAGPGGHIVTGAN
                     SKIATVDQPGEY"
     gene            656..910
                     /locus_tag="PL106_01890"
     CDS             656..910
                     /locus_tag="PL106_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31848.1"
                     /translation="MENCRSAGEAATTEIARNRDAQGFPENKKAARAGGEVAGKARKD
                     LETKSGRKVVTSENYRSLTQGARKQVRLSAAPTKKRKKGK"
     gene            complement(1067..1252)
                     /locus_tag="PL106_01895"
     CDS             complement(1067..1252)
                     /locus_tag="PL106_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31849.1"
                     /translation="MIFRPDSDVDIMVEFERPIGMEFFDLEQDLADIVSRKVDLVMRG
                     AVKPHYMEFIQKDLRYV"
     gene            complement(1230..1358)
                     /locus_tag="PL106_01900"
     CDS             complement(1230..1358)
                     /locus_tag="PL106_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31850.1"
                     /translation="MGTLADILAVLRHERARLFVKYGLKSMAVFGSATRDDFPAGQ"
     gene            1398..1817
                     /locus_tag="PL106_01905"
     CDS             1398..1817
                     /locus_tag="PL106_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA31851.1"
                     /translation="MPKPNKTKATTASVAAFIDKQPKDEVRDDCRALMNVMHELTGEE
                     PYMYGPSIIGYGTYHYKYASGHEGDAPLLAFSPRKPELVIYLYQEVVGPELLSKLGKH
                     RATKGCVYIKRLADIDLKVLKTLFKGSIAAVRKAFPQ"
     gene            complement(1821..2804)
                     /locus_tag="PL106_01910"
     CDS             complement(1821..2804)
                     /locus_tag="PL106_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNA31852.1"
                     /translation="MKQLFKYLGILLLVAIAGIVIAITYITQALPDIPVQADLKVEIT
                     PERVARGEYLANSVCVCMDCHSTRDWNSFSGPLVPGTLGKGGERFDQSMNFPGVFYAR
                     NLTPFGLKDWTDGEIYRAITSGVSKDGHPFFPVMPYPNYNKLATEDVHSIIAYLRSLP
                     PIESAPGASKADFPLNIIMHTIPEEAHPAKRPDPSNSVAYGAYMANASGCIVCHTRHE
                     KGSPVGEPGAGGFDFKFPTGSIVRSANITPHADGIGSWDKATFISRFKQYADSSYVPP
                     TVDWKANDFQTVMPWTMYCHLTEQDLGAIYDYLHTLPPVAGKVEKWSEPGS"
     gene            3314..>4686
                     /locus_tag="PL106_01915"
     CDS             3314..>4686
                     /locus_tag="PL106_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA31853.1"
                     /translation="MTDTLTAPRLFRPHNLINGTWSSAGDGSFNTVVDKYHGTVLARL
                     PNATEAQMEEAIAAAYASREVLRKMSAGERSAKLEGLATLIAEHGDELAQLIVQEGGK
                     PIGYAKTEIARCITTVRTAAAEALRFGGEVVPMDHGAGTGRTAITKRFPVGVVAAITP
                     FNFPLNLVLHKVAPAMAVGCPVVLKPAPQAPLCALVLGGFMERIGWPKGAFNVLMCGV
                     PVAEKLVRDERVAMLSFTGSDKVGWHLKSICGKKKVALELGGNASVIVDEGTDLVAIA
                     KSVAMGANLYAGQTCISTQRIYALKSVFSRFRDLLVREFELLGSGDPADAGVSVGPII
                     DKGHFERISSWVDEAVKGGAKLLAGGKVIDAAHNVYAATLLTGTKNTMKVNCAEVFGP
                     VAVLEEVADFGEALAAVNDSAYGLQAGVFTNNIAHMKRAHDELEVGGIIINNVPGFRI
                     DPMPYGGV"
BASE COUNT          929 a         1370 c         1487 g          900 t
ORIGIN      
        1 gtattcgccg ggctggtcga cggtagcaat tttcgagttg gctccggtga cgatatggcc
       61 gcccggccct gccgtccatt catacgtgga aaaagcaggc tggctcgaat tgccgatgag
      121 cgtcgacgtg cccgagccgc agcccagcgg cgtgggcgga ttgatccagg ctgccagctg
      181 gttgggactt tcagcgacgg taaccgttgc cgttttggta cacgtcacga ggccgttgtc
      241 gtaagtaact gtcaatgtgt atgttccggc ggcattcacg acaggcgaga gggtgttttg
      301 ccccgaaaca atattgccgt tcggggagtc ccagttgtag gtaatgtttg cgccggtgct
      361 cgatcccgct ccgctaaggg tgatgctggc gccgtcgcag gggatgaagg agatggcggg
      421 cgacgcggcg gcggtgacgc ttaccacatg cacagtgacg gtatccttta ccatgcatgt
      481 cggggcgaac aggatatcgt cgagtgcgaa atcgttgccg ccgagcgttg tgttctgatt
      541 aacgatacag atcgtggcgc tgctgttgct gccgctgttc cagagctgga aaaactgctg
      601 ccagttgcag gtgcctcccg gcgcggcaaa gatgttgccg agcgggctgc cgttgatgga
      661 aaattgcagg agcgccggcg aagcggccac caccgagatc gcccgcaacc gtgatgccca
      721 aggcttcccc gagaacaaga aggccgcccg tgccggtggc gaggtcgcag gcaaggctcg
      781 caaggacctg gaaacgaaga gcggacgcaa ggtggtgacg agcgagaact accggtcgct
      841 cacccaggga gctaggaagc aggtgcggtt atccgctgcg ccaacaaaga agcgtaaaaa
      901 ggggaagtag ttcgcggaca atactgtacc tgcatggcaa ccaagcccaa caagacccac
      961 gccaccaccg ccagcgtcga taccttcgtt cggtacccgc atctatcttg ttcaacccgc
     1021 tcctgcgact tcgcgcatat ccacgagccg caccttgtcg ctgcgctcag acataccgca
     1081 gatccttctg gatgaactcc atgtaatgcg gcttcaccgc gccccgcata accagatcca
     1141 cctttcgcga cacgatgtcg gcaaggtcct gctcaaggtc gaagaactcc atcccgatcg
     1201 gccgctcgaa ctccaccatg atgtccacat cactgtccgg ccggaaaatc atcgcgcgtg
     1261 gcggagccga acacggccat gctcttcagg ccgtacttga cgaaaaggcg ggcacgttcg
     1321 tggcgcaaca cggcgaggat gtcggccagg gtgcccatgg ttgcacaaat atccgacgag
     1381 ccgactgacc ttcgcgtatg cccaaaccca acaagaccaa agccaccacc gccagcgtgg
     1441 ctgccttcat cgacaagcag ccgaaggatg aggtgcgcga tgattgccgc gcgttgatga
     1501 acgtgatgca cgagctcacc ggcgaggagc cgtacatgta cgggccttcg atcatcggtt
     1561 atggcaccta tcattacaag tatgccagcg gccacgaggg cgatgcaccc ttgctcgcgt
     1621 tcagcccgcg caaaccggaa ctggtgatct acctgtatca ggaggtggtg ggcccggagc
     1681 tcctctccaa gctgggcaag cacagggcca ccaagggctg cgtgtacatc aagcgcctcg
     1741 ccgacattga cctgaaggtg ctgaagacct tgttcaaggg atcgatcgcg gcggtgcgga
     1801 aggcctttcc gcaataggtg ttacgagccc ggctcgctcc acttctccac tttgcccgcc
     1861 acgggcggca gggtatggag gtagtcgtag atggcaccaa ggtcctgttc cgtcaagtgg
     1921 cagtacatcg tccatggcat cacggtctgg aagtcgttgg ctttccagtc cacggtaggt
     1981 ggcacgtaac tgctgtccgc atattgtttg aatcgcgaga tgaacgtggc cttgtcccat
     2041 gatccaattc catccgcatg cggcgtgatg ttggccgaac gcacgatgga tcccgtgggg
     2101 aatttgaaat caaagccgcc cgctcctggc tcacccaccg gacttccctt ttcgtgcctg
     2161 gtatggcaaa cgatacatcc gctcgcgttc gccatataag caccgtaagc aacactgttc
     2221 gatgggtccg ggcgtttggc gggatgcgct tcttcaggta tggtgtgcat gatgatgttc
     2281 aaggggaagt cggccttgct ggccccgggg gcactctcta tcggcggcag gctgcgcagg
     2341 taagcgatga tgctgtgtac atcctccgtg gccagcttgt tgtagttcgg atagggcatt
     2401 accggaaaga agggatggcc atccttgctt acaccgctgg tgatggcgcg gtagatctcg
     2461 ccatcggtcc agtccttcag gccgaatggg gtgaggttgc gcgcatagaa cactccgggg
     2521 aagttcatgc tctggtcgaa ccgctcgccg cccttgccca acgtgccggg caccaacgga
     2581 ccgctgaagc tgttccagtc gcgtgtgcta tggcagtcca tgcacacgca cacactgttg
     2641 gccaggtact cgccgcgcgc cacacgctcg ggtgtgatct ccaccttcag gtcggcctgt
     2701 acggggatgt ccggcagggc ttgggtgatg taggtgatcg cgatcacgat ccctgcgatc
     2761 gcaacgagca ggaggatgcc gaggtacttg aacagttgtt tcatgggaga gggagttggt
     2821 acccaagatt acaacatatt gaccatatcg cgatcacctg ccttccgggc acctgttttt
     2881 ttgcgcgtga ccgggtgcct ggtgggcaga cctgatgcct tacctgtccg tcggtacatg
     2941 cctcccctgg tcatgtggca ccctgctccg gacggcccat gccgcagtgt gcggtcaata
     3001 tcgtcacggc cttgtggttt ttggtcaacg gagcgcccgt tcggatcctt gttcaggatg
     3061 gccttcgaga ctgttttgcc caagcatggc aaaatgaaga atgcaggacg gttcatacgg
     3121 gaccgttctt ggtgaagcat gggcggccac gggaccggag gatgcttgcg aagggatccc
     3181 caacaggcag agatgcattc gaggtgatcg ggggcggcct tcgaccggcc attcggccga
     3241 gaatgaagag ttcttgttcc ggagccggta ctttcgccgc atcattgcca catcatgggc
     3301 ggtgatccga gccatgaccg acaccctcac cgccccgaga ctcttccgcc cccacaacct
     3361 gatcaacggt acctggtcct ccgccggtga cggcagtttc aacaccgtgg tggacaagta
     3421 ccacggcacc gtactggcac gactgccgaa cgctaccgag gcgcagatgg aagaggcgat
     3481 cgcagccgct tatgccagcc gggaggtctt gcgcaagatg agcgcggggg agcgcagtgc
     3541 gaaactggaa gggctcgcaa cgctgatcgc tgaacacggg gacgaactcg cacaactcat
     3601 cgtgcaggag ggtggcaagc ccattggcta cgcgaaaacg gagatcgcgc ggtgcatcac
     3661 cacggtgcgt accgcagcgg ctgaggccct gcgcttcggg ggtgaggtgg tgcccatgga
     3721 ccacggtgcg ggcacgggcc gtaccgcgat caccaagcgt tttccggtcg gcgtggtcgc
     3781 cgcgatcacg ccgttcaact tcccgctgaa cctggtgctg cacaaggtgg cgcccgccat
     3841 ggccgtgggc tgccccgtgg tgctgaaacc cgccccccag gcaccgctct gcgcactcgt
     3901 cctcggaggt ttcatggaac gcatcgggtg gcccaagggc gcgttcaacg tgctgatgtg
     3961 cggcgtgccc gtggccgaga agctggtacg cgatgagcgc gtggccatgc tcagtttcac
     4021 ggggagcgac aaggtgggct ggcacctcaa gagcatttgc ggcaagaaga aagtggcact
     4081 ggagctgggc ggcaacgcga gtgtgatcgt ggatgagggc accgacctcg tcgccatcgc
     4141 caagtcggtg gccatggggg ccaacctcta cgcagggcag acctgcatca gcacacaacg
     4201 catctacgcg ctcaagagcg tgttcagtcg gttccgcgat ctgctggtga gggaattcga
     4261 gctactggga tcaggggatc cagcggatgc tggcgtgagc gtcggcccga tcatcgacaa
     4321 ggggcatttc gagcgcatca gcagctgggt ggacgaggcc gtgaaaggag gggccaagct
     4381 gctcgcgggc gggaaagtga tcgatgctgc acacaacgtg tacgcggcca cgttgttgac
     4441 cggcaccaag aacaccatga aggtgaactg cgccgaggtt ttcgggccgg tggccgtgct
     4501 ggaggaggtg gccgacttcg gtgaggcgct cgccgcggtg aacgattccg cctacggcct
     4561 gcaagccggt gtgttcacca acaacatcgc ccacatgaaa cgcgcgcacg atgaactgga
     4621 ggtcggtggc atcatcatca acaacgtgcc cggcttcagg atcgatccca tgccctatgg
     4681 tggcgt
//
LOCUS       DAOQOC010000072         4673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7876_length_4673_cov_3.199220, whole genome shotgun sequence.
ACCESSION   DAOQOC010000072 DAOQOC010000000
VERSION     DAOQOC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7876_length_4673_cov_3.199220"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..854
                     /locus_tag="PL106_01920"
     CDS             153..854
                     /locus_tag="PL106_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA31854.1"
                     /translation="MAATKTNPPAEDKDLDIGEIYTRTELFFEKNKKLVTGIVAAVLL
                     LVGGFLGYKKFVVEPKAKEAQELIWKAQYWFEIDSLDLAINGDGGSYYGFQHIADNYG
                     GTPAGEVANFYLGVCYHQKGEWETALNYYKEADLDDDILRVMAVGNQGDVLVELGRPD
                     EAISQFEKAANMVRSDYTTPMFLWKVGVLYRQKNDHEHAAKCFRRIATDFPASPDVQQ
                     ARKYAAYSEAMTGKS"
     gene            857..1351
                     /gene="ribH"
                     /locus_tag="PL106_01925"
     CDS             857..1351
                     /gene="ribH"
                     /locus_tag="PL106_01925"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNA31855.1"
                     /translation="MATAGKDLSSYHTASVPGGSGLRLGLVTSEWNPEITGALRDGAV
                     ETLLKHGVALNLLLEERVPGSFELPLGAQTLLEHRDVDGVICIGSVVRGETPHFDYVC
                     QAVASGIMDLNIKHGKPVIFCVLTDDTMEQARDRSGGRHGNKGVDCAVALLKMCALTR
                     KYRG"
     gene            complement(1373..2014)
                     /locus_tag="PL106_01930"
     CDS             complement(1373..2014)
                     /locus_tag="PL106_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31856.1"
                     /translation="MRAVAVVLLLCGITPAMAQKKIPEHKPTVQGTFFLPTALDNKMF
                     TVLTSPLGEGELFVQIPLWKGFGIGLGAKVMGWDLKNNAFSQLDTHGDASRWTGALKV
                     QYAHYTGPITFYEAAVRAGYSRWTWDCSTCESNTFQGGIHLAGTFGYYVHASKNLAFG
                     IILGYETDAARFTPDLLCLNDFPGYEGMTGPYRFFTVGLGFSTRFEKDSEERW"
     gene            complement(2011..2394)
                     /locus_tag="PL106_01935"
     CDS             complement(2011..2394)
                     /locus_tag="PL106_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31857.1"
                     /translation="MHLLDSLRPALLLSLSILFLLMLWRRFKHKAMMAERPVAAHAEL
                     LALHVEYHPERLRAEVAMPEDGELRPALLDGEHRPLFAWPRMNGKAGTQVLELPLTDH
                     ADGTYYFELATATQRTVRRFILNRS"
     gene            complement(2398..2673)
                     /locus_tag="PL106_01940"
     CDS             complement(2398..2673)
                     /locus_tag="PL106_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HNA31858.1"
                     /translation="MLNQETKERLRQSLDKVHDWPSVYMFKLIFEPEPGRLERVQALF
                     PPEAELLRKYSTGGKYLSITVREVMINADEVVARYEKASEIEGVIVL"
     gene            2804..3937
                     /locus_tag="PL106_01945"
     CDS             2804..3937
                     /locus_tag="PL106_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31859.1"
                     /translation="MTLTPSHAVTFLALFLPALVLQAQNVPSTSGDAAPVGQMAPARD
                     GWQGAWSTTDARRAAETWKALSEARPSDVNAQFNWFRSERNARLGSNNGRLQEKDNIQ
                     LETIAGRIRNAAPGSFEQHLASYYLAFPGPEAFTELDAAHAAGAGRTELILPMLTRAN
                     LSGDKAAQDHWSAQLEQRGGLSPALADVASDLLLSTGEGGILFTNGDMDGAPALMLQR
                     LHDQRRDVLLVDQRLLNDMGYRQRIWKEAKATGPVPAAGPAFARALSTATERPVFLAL
                     SLDRSWFDAFPEGLYTSGIAFAVGGTPPDGRSLAERWDRMKKTTAAGPLSRNYLLPGS
                     VLLQQYRSNGDEAHASRLEHELRGLATRLGATQDLIKAGVFTH"
     gene            3947..4543
                     /locus_tag="PL106_01950"
     CDS             3947..4543
                     /locus_tag="PL106_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA31860.1"
                     /translation="MLRTDHQAQSDERLMELIVRGDERAFGALYDRYQRKLISYFARM
                     LWNDRERAQDLLQELFTRIARQPGSYDPARPFRTWLYSVANNMCKNEYRHEAVRKAAV
                     PHLRAGYNGTVGAAHGDAVDRERFRERLDAELDRLDPDQKATFVMRYHEDMAIKEIAI
                     AFGISEGTVKSRLFYTLKKLAERLKEFGPNATIRHGTA"
BASE COUNT          943 a         1438 c         1501 g          791 t
ORIGIN      
        1 gatcacgtcc tacggcaaca aggtacatgg ggcaggcagg gaggaggtca agctcaagaa
       61 aatgccccaa atccctgttt tgcaagccca tacgcagatg tcgcgggaag ctttactttc
      121 gcgcccccgc cggaggaccc ggcaaacgac gcatggccgc taccaagacc aacccgcccg
      181 ccgaggacaa ggacctcgac atcggcgaga tctacacaag gaccgaactg ttcttcgaga
      241 agaacaagaa gctcgtgacc ggcattgtgg ccgcagtgct gctgctggtg ggcggcttcc
      301 tgggctacaa gaagttcgtg gtggagccga aggccaagga ggcgcaggaa ctcatctgga
      361 aagcccagta ctggttcgag atcgattcgc tggacctggc gatcaacggg gatggaggca
      421 gctattatgg gttccagcac atcgccgaca attacggggg cacaccggcg ggcgaagtgg
      481 ccaacttcta cttgggcgtg tgctaccacc agaagggcga atgggaaacg gccctcaact
      541 attacaaaga agcggacctg gacgacgaca tcctcagggt gatggcggtg ggcaaccagg
      601 gtgatgtgct ggtggaactc ggacgaccgg atgaggccat cagccagttc gagaaagccg
      661 ccaacatggt gcgcagcgac tacaccacgc ccatgttcct atggaaggtc ggcgtgctgt
      721 accgccagaa gaacgaccac gagcacgcgg cgaagtgttt ccgccgcatc gccacggatt
      781 ttcccgcatc gcctgatgtg cagcaagcgc ggaaatacgc ggcctacagc gaggccatga
      841 ccggcaagag ctgatcatgg ccacggccgg taaggacctg tcctcctacc acacggccag
      901 cgtacccggt ggatcgggct tgcgcttggg cctggtcacc agcgaatgga acccggagat
      961 cacgggtgcg ctgcgcgatg gcgcggtgga aaccctgctc aagcacggcg ttgcgttgaa
     1021 cctgctcttg gaggaaaggg tgccgggcag cttcgaactg cccttggggg cacaaacgtt
     1081 gttggaacac cgcgacgtgg atggtgttat ctgtatcggc agcgtggtgc gtggcgaaac
     1141 gccccacttc gactacgtgt gccaagcggt ggccagcggc atcatggact tgaatatcaa
     1201 gcacgggaag ccggtcattt tctgtgtact caccgacgat acgatggagc aggcgcggga
     1261 ccgcagcggt gggcggcatg gcaacaaagg ggtcgattgt gcggtggccc tgctgaagat
     1321 gtgcgcgctg acgcggaagt accgcgggta actctcggga caggcattcc gcctaccaac
     1381 gctcctcgct gtccttttcg aaacgggtgc tgaaccccag gcccaccgtg aagaaccggt
     1441 acggcccggt catgccctca tacccgggga aatcattcag gcacagcaga tcgggcgtga
     1501 agcgggccgc atcggtctca taaccgagaa tgataccgaa cgcgagattc ttcgaagcat
     1561 gcacatagta cccgaaggtt ccggccaggt ggatcccgcc ctggaacgtg ttgctttcgc
     1621 acgtactgca gtcccaggtc cagcgactgt agcctgcacg caccgcggct tcgtaaaagg
     1681 tgatgggccc cgtgtagtgc gcgtattgca ccttgagtgc tccggtccag cggctcgcat
     1741 cgccatgcgt gtccaactgc gagaaggcgt tgttcttcag gtcccagccc atcaccttgg
     1801 cgcccaggcc gatgccgaag cccttccaga gcgggatctg cacgaacaac tcaccctcgc
     1861 cgagtggtga ggtgagcacg gtgaacatct tgttgtccag cgcggtgggc aggaagaaag
     1921 tgccttggac cgtgggcttg tgttccggta tttttttctg ggccatggcc ggggtgatcc
     1981 cgcacagcag cagcaccacg gcaacggccc tcatgatcgg ttcaggatga atcgacggac
     2041 cgtacgctgg gtggccgtgg ccagttcgaa ataatacgtc ccgtccgcat ggtccgtcag
     2101 tgggagttcc agcacctgcg taccggcctt cccgttcatc cgcggccagg cgaacagggg
     2161 ccggtgctca ccatcaagca aggcgggccg taactcccca tcctcgggca tggccacttc
     2221 ggcacgcagg cgctcgggat ggtactccac atggagcgcg agcaactcgg catgtgccgc
     2281 cacgggccgt tcggccatca tggccttgtg cttgaaccgg cgccacagca tcaacaggaa
     2341 gaggatgctg agcgaaagaa ggagcgcggg gcgcagcgag tcgaggagat gcatggatca
     2401 gagcacgatc accccctcga tctccgaggc tttttcgtag cgtgccacca cttcgtccgc
     2461 gttgatcatc acctcacgca cggtgatgct caggtacttg ccaccggtac tgtacttgcg
     2521 caacagctcg gcctcgggag ggaaaagggc ctgtacccgt tccaatcgcc cgggctcggg
     2581 ctcgaagatg agcttgaaca tgtagacgct gggccaatca tgcaccttgt cgaggctctg
     2641 gcgcaatcgt tccttggtct cctggttgag catggcgctc aaaggtaccc cgacaggtca
     2701 ctgtccgatc cgtggacgtg ccatcccgtc ccgggatcgg aagggaaccc gacctgcatg
     2761 aactttgcgc cacgctgcgt gtacggcgca tcgtatgacc ggcatgaccc tgactccttc
     2821 ccacgcggtg acctttctgg ccctgttcct gccagcactt gtgctgcagg cccagaacgt
     2881 cccgtccacc agcggcgatg cggcacccgt tggtcaaatg gctccggcgc gggacggatg
     2941 gcagggagcg tggtcaacaa cggacgcccg ccgggccgcc gagacctgga aagcgctgag
     3001 cgaagcccga ccgtcggatg tgaacgccca gttcaactgg ttccgcagcg agcgcaatgc
     3061 acgcttgggg agcaacaacg gacgcctgca ggagaaggac aacatccaac tggagaccat
     3121 cgccggacgg atacgcaacg cggcaccggg cagcttcgaa cagcacttgg cgagctacta
     3181 cctggctttc cccgggccgg aggcgttcac cgaactggat gcggcccatg ccgctggagc
     3241 cggacgcacc gagttgatcc tgcccatgct cacccgggcg aacctcagtg gcgacaaggc
     3301 ggcgcaggac cattggagcg cgcagcttga acaacgtggc ggcttgtccc cggccctcgc
     3361 ggatgtggcg agcgacctgc tcctgagcac gggcgagggc ggcatccttt tcaccaatgg
     3421 cgatatggac ggcgcgcccg ccctgatgct gcaacgcctg cacgatcagc gacgggacgt
     3481 gctgctggtg gaccaacgcc tgctgaacga tatgggctac cgccagcgca tctggaagga
     3541 ggcgaaggcc accggtccag tgcccgccgc cgggccggcc ttcgcgaggg cgctttccac
     3601 ggctaccgaa cgtccggtgt ttttggcatt gagcctggac cgttcctggt tcgatgcatt
     3661 cccggagggc ttgtacacct cggggatcgc cttcgccgtg ggcggaacac cgcccgatgg
     3721 gcggagcttg gccgagcgat gggaccgcat gaagaagacc acggccgcgg gaccgctgag
     3781 ccgcaactac ctcttaccgg gatcagtact tctgcaacag taccgatcca atggcgacga
     3841 ggcgcacgca tcgcgattgg agcatgagtt gcgcggcctg gcgacgcggc ttggcgccac
     3901 gcaggacctg atcaaagccg gggtattcac ccactgaaca atggggttgc tccgcaccga
     3961 tcaccaggca cagagcgacg agcgactgat ggagttgatc gtacgcgggg atgagcgcgc
     4021 gttcggagcg ttgtacgacc ggtatcagcg caagctcatc agctacttcg cgcgcatgct
     4081 gtggaacgac cgggagcggg cgcaggacct gctgcaggag ctcttcacca ggatcgcgcg
     4141 gcagcccggg agctacgatc ccgctcgccc gttcaggaca tggctgtaca gcgtggccaa
     4201 caacatgtgc aagaacgagt atcgccacga agcggtgcgc aaggctgccg tgccgcacct
     4261 gcgggccggg tacaacggca ccgttggcgc ggcacacggc gatgcggtgg accgcgagcg
     4321 tttccgcgag cgcctggacg ccgaactgga ccgtttggac cccgaccaga aggccacctt
     4381 cgtgatgcgc taccacgagg acatggccat caaggagatc gccatcgcct tcggcatctc
     4441 cgagggcacg gtgaagtcgc gcctgttcta caccctgaaa aaactggccg aacgcttgaa
     4501 ggagttcggc cccaacgcaa cgatccgcca tggaaccgcg tgaacgatac cagcccgagg
     4561 atattgaagc cctgctgcac gagcgcggtt tcgacgagct gctggacgag gagcgggcct
     4621 tcgtgttgcg ccacctgagc ggccgcgacg agtacgaagc catgcgttcc ctg
//
LOCUS       DAOQOC010000073         4660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7911_length_4660_cov_4.080347, whole genome shotgun sequence.
ACCESSION   DAOQOC010000073 DAOQOC010000000
VERSION     DAOQOC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7911_length_4660_cov_4.080347"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PL106_01955"
     CDS             complement(<1..181)
                     /locus_tag="PL106_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31861.1"
                     /translation="MCGIVGYMGDKDAYPILINGLRRLEYRGYDSAGVALLDGSTLNV
                     YKCQGKVSDLEAHVAS"
     gene            complement(194..1543)
                     /locus_tag="PL106_01960"
     CDS             complement(194..1543)
                     /locus_tag="PL106_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNA31862.1"
                     /translation="MKWTDWLRCARPSSKAVLLLAVMAVVACRKPEEDIGLDLLPGED
                     VLGSVVMDTTRILAWTTEPQTVRTSALTRNLLGTLLDPDFGTVTTGMAFQVRLSSSNV
                     GPYDATNVCDSLVLSLAYEIGNYGYGDRHEQGFHVFRLAEDLSTDSTYRADDVPLSLW
                     GDLVGGTRNSFPIEPLQGPVIAGDTLVPQLRLPLKKELGNELLSHWGGDELVDNGLEH
                     FLGYFKGLLVVPRAEAEVPYQQAVLHFNLLNPQSKLTLYYHNAEDTLQYDLVINTSSV
                     RYTVGKSDHTKALRPQLPAALADSTLGQQRVYLQALGGLKGELRFPGLENYRHSGMGA
                     LAKAELIMPVDGDVFPLYPPPGQIFVFRKDAEGNDAVIPDQQVSGNQVGGLYDADNKE
                     YRLVITQWLQGVISGEYPNTGLSLVPGSNGVSVNRVILAGPENPVDRMRLRLTFTTY"
     gene            complement(1547..2368)
                     /locus_tag="PL106_01965"
     CDS             complement(1547..2368)
                     /locus_tag="PL106_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNA31863.1"
                     /translation="MSLKKAKILTVSQFIEPFTDGTEMGLAARQLPQGMLDKGNEIRV
                     FMPKWGNINERRHQLHEVIRLSGMNLIIDDTDHALLIKVASVPSARMQVYFIDNEEYF
                     KRKAILNDENEAFYQDNDERALFFCRGVLETVRKLGWKPDIIHCHGWMTSFVPLYVRH
                     LFNDDPHFEDAKLVFTAYDDPAENLNKDLAKKLTQEGFPKELLKSLVKPTTEDLSKFA
                     MGLCDAVTKGDAKLSRVMENAFKATDKRVLAYASEEERVAAHAEFYHEVLEEALV"
     gene            2467..3315
                     /gene="panC"
                     /locus_tag="PL106_01970"
     CDS             2467..3315
                     /gene="panC"
                     /locus_tag="PL106_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNA31864.1"
                     /translation="MRILAKATDAAVWSAAVRREGRSIGFVPTMGALHQGHLTLVARA
                     KAECDAVCVSIFVNPLQFNNPDDLRHYPRQLEQDRGLLTGAGCDMLFVPEEASIFQEH
                     SPRLFDLGGQDNILEGPSRPGHFQGVVNVVERLFHYIRPDAAFFGEKDRQQLAVIQHV
                     AHTLHWPERIVPCPTERAPDGLALSSRNLRLSPQERAVAPVLYQCLQSVAGSAFREPV
                     TSAKEHGLRLLRAAPSIRLEYLEISDPLSLEPLVDWGDRTEAVALVAAWLGPVRLIDN
                     VTLHKR"
     gene            3379..3732
                     /gene="panD"
                     /locus_tag="PL106_01975"
     CDS             3379..3732
                     /gene="panD"
                     /locus_tag="PL106_01975"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNA31865.1"
                     /translation="MTIEVLRTKIHRITVTEANVDYIGSITLDEDLIDASGLVEGEKV
                     TVLDITNGERLDTYVIKGERGSGMVCMNGPAALRVKVGDVVIVLSYARMSVEEARVFR
                     PTIVFPNERTNRLKG"
     gene            3729..>4660
                     /locus_tag="PL106_01980"
     CDS             3729..>4660
                     /locus_tag="PL106_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNA31866.1"
                     /translation="MKRSIIGILKIAVPLGIGLWLVISTYSDLNNTQKNELFEAFRQA
                     DPGWLLLATGIGWLAHVSRGWRWRYLLEPLGHRPGAINCYHAVMIGYLMNMFIPRAGE
                     ASRAVSLYRTDKVPFEQGFGTILAERVIDTVMLAGIGLAAVLFQYDKLDLFQARIEAY
                     RASQGPAAGSGFPWGTVLMIALALVVVAGVYLLATRAAMRTRAIDLLRGFAEGLKAIL
                     STRRKAAFLGHTLFIWAAYLAMFVIGFQCLPSMKDVPVAGILAGFIAGAIGIVLVQGG
                     IGVYPAFVALIVGVYMAPPAGGGLLRPDALAMGWL"
BASE COUNT          839 a         1496 c         1399 g          926 t
ORIGIN      
        1 tggaagccac gtgcgcctcc agatcgctca ccttgccctg gcatttgtac acattgagcg
       61 tgcttccatc cagcagggcc acgccggcgc tgtcgtagcc ccggtactcc agcctgcgca
      121 gcccattgat caggatggga taggcgtcct tatcgcccat atagccgacg atgccgcaca
      181 tgttcggtca ggttcagtag gttgtgaagg tcaggcgaag gcgcatccta tcgaccgggt
      241 tctccggccc cgccaggatg acccggttga cggaaacgcc gttgctgccc ggtacgagac
      301 tcaatccggt gttcggatac tccccgctga tgacaccttg cagccactgg gtgatcacca
      361 gccggtattc tttgttgtcc gcgtcgtaga gcccacccac ctggttgccc gacacctgct
      421 ggtcggggat cacggcatcg ttcccttcgg cgtccttgcg gaagacgaag atctggcccg
      481 gtggcggata gagcggaaaa acatccccat ccaccggcat gatcaactcg gccttggcca
      541 gggcgcccat gccgctgtgc cggtagttct ccagcccggg gaaacgcaac tcgcccttca
      601 ggccgcccag ggcttgcagg tacacgcgct gctgccccaa ggtactgtcg gccaatgccg
      661 cagggagttg cggtcgcagg gccttcgtgt ggtcactctt tcccaccgtg taccgcacgc
      721 tgctggtgtt gatgacgaga tcgtactgga gcgtatcctc cgcgttgtgg taatacaggg
      781 tgagtttgct ctgcgggttc agaaggttga agtgcagtac ggcctgttgg tagggcactt
      841 cggcctccgc ccgtggtacc accagcaatc ccttgaaata acccaggaaa tgttccaggc
      901 cattgtccac cagttcgtcg ccgccccagt ggctcaacaa ttcgttgccc agttcctttt
      961 tcaacggcag ccgcaactgt ggtacaagcg tgtcgcccgc gatcaccgga ccttgcaagg
     1021 gctcaatggg aaaggaattc cgcgttccgc cgaccaggtc gccccacagg ctcaaaggga
     1081 cgtcatctgc ccggtacgtg gaatcggtgc tcaggtcctc ggcgaggcgg aagacgtgga
     1141 acccctgttc atgtcgatcg ccatagccat agttgcctat ctcatacgcc agggagagca
     1201 ccagtgaatc gcacacgttg gtagcatcgt acggccccac attgctgctg ctcaatcgca
     1261 cctggaaggc catgcccgtg gtcaccgtgc cgaagtccgg gtccagcaag gtgcccagca
     1321 ggttgcgcgt gagcgcgctg gtccgtaccg tctgtggttc cgtggtccat gccaggatac
     1381 gcgtggtgtc catgaccacg gagcccaata cgtcctctcc gggcagaagg tccaagccga
     1441 tgtcctcctc gggcttgcgg caggccacca cggccattac cgccaaaagc agcacggcct
     1501 tgctggaagg ccgtgcgcac cgcaaccaat cggtccactt cacgcctcaa acaagcgctt
     1561 cctccaggac ctcgtgatag aattccgcgt gagccgccac acgctcctcc tccgatgcat
     1621 aggccaatac ccgcttatcg gtcgccttga aggcgttctc catcacgcgg ctcaacttgg
     1681 catcgccctt ggtcaccgca tcacacaagc ccatggcgaa cttgctcagg tcctcggtgg
     1741 tgggcttgac aaggctcttc agcaactcct tggggaaacc ctcctgggtc agctttttcg
     1801 caaggtcctt gttcaggttc tccgccgggt cgtcatacgc ggtgaacacc agtttggcgt
     1861 cctcgaagtg ggggtcgtcg ttgaagaggt ggcgcacata caggggaacg aaggaggtca
     1921 tccagccatg gcagtggatg atgtccggct tccagcccag cttgcgcacc gtctccagca
     1981 caccccggca gaagaagagc gcacgctcat cgttgtcctg gtaaaaagcc tcgttctcgt
     2041 cgttcaggat ggccttccgc ttgaaatact cctcgttgtc gatgaagtac acctgcatgc
     2101 gggcgctggg cacactggcc accttgatca gcagtgcgtg gtccgtgtcg tcgatgatca
     2161 ggttcatccc gctgaggcgg atgacctcgt gcagttggtg ccgacgctcg ttgatattgc
     2221 cccatttggg catgaacacc cggatctcgt tgcccttgtc cagcatgccc tggggcagct
     2281 ggcgggcggc aaggcccatc tccgtcccgt ccgtaaatgg ctcgatgaac tgcgatacgg
     2341 tcaggatctt cgctttcttc agactcatgt gcgggcaggg gttagaggat cacaaaagta
     2401 aagacattcg gagccgtttt caaagtaccg gccttgccgc accccactcc agaacccctt
     2461 gccgggatgc gcatcctcgc aaaagccacg gatgctgcgg tctggagtgc tgccgtgcgg
     2521 cgcgaaggcc gcagcatcgg tttcgtacct accatgggcg cgttgcacca gggtcacctc
     2581 acccttgtag cacgggccaa ggccgagtgc gatgccgtgt gcgtcagcat cttcgtcaac
     2641 cccctgcagt tcaacaaccc cgatgacctg cggcactacc cccgccaatt ggaacaggac
     2701 cgaggcctgc tgacaggagc cggttgcgat atgctcttcg ttcctgaaga agcctccatc
     2761 ttccaggaac acagtcccag acttttcgac ctcggtgggc aggacaacat tcttgaaggc
     2821 ccatcgcgac ccggtcattt ccagggtgtt gtgaacgtag tggagcgcct cttccactac
     2881 atacggcccg atgctgcttt tttcggcgaa aaggaccggc agcaattggc cgttatccaa
     2941 catgtggcgc acaccctgca ctggcccgag cggatcgtgc cctgccccac cgagcgcgcg
     3001 cccgacgggc tggccctcag cagccgcaac ctgcgcctga gcccccagga acgcgccgtg
     3061 gccccggtgt tgtaccagtg cctgcagtcc gtggccggat ccgcctttcg tgaaccggtg
     3121 accagcgcga aggagcacgg cctgcggctg ttgcgcgccg caccctccat tcggctcgaa
     3181 tacctggaga tctccgaccc gctgagcctg gaaccccttg tggactgggg tgaccgcact
     3241 gaagcggtag ccttggttgc ggcctggttg ggtccggtac ggctgatcga caatgtcacc
     3301 ctccacaagc gctgagccgc agccaccacg tgggcggccg aagacggtaa gttcgcgccc
     3361 catttccggt atccgaacat gaccatcgaa gtcctgcgaa ccaagatcca ccgcatcacc
     3421 gtgaccgaag cgaacgtgga ttacatcggc agcatcacgc tcgatgagga cctgatcgat
     3481 gccagcggcc tggtggaagg cgaaaaagtg accgtgctgg atatcaccaa tggcgagcgg
     3541 ctggacacgt atgtgatcaa aggtgagcgc ggcagcggca tggtgtgcat gaacggcccg
     3601 gccgccctgc gcgtgaaggt gggcgatgtg gtcatcgtcc tttcctatgc ccgcatgagc
     3661 gtggaggagg cccgcgtctt ccggcccacg atcgtctttc ccaacgagcg aacgaacagg
     3721 ctgaaggggt gaagcggtcg atcatcggca tcctgaagat cgccgtgccg ctcggcatag
     3781 ggctctggct ggtgatcagc acgtacagcg acctgaacaa tacccagaag aacgaattgt
     3841 tcgaagcctt ccggcaggcc gacccgggct ggctcctgct ggccaccggc atcggctggc
     3901 tggcccatgt gagccggggt tggcgctggc gatacctgct ggaaccgctg ggtcaccggc
     3961 ccggtgccat caattgctac catgccgtaa tgatcggcta cctcatgaac atgttcatcc
     4021 cccgtgcagg ggaggccagc cgggccgtgt ccctttatcg cacggacaag gtgccgttcg
     4081 agcagggctt cggcaccatc ctggccgagc gggtgatcga caccgtcatg ctggcgggga
     4141 tcggtcttgc agccgtgctt ttccagtatg ataagctgga cctcttccag gcgcgcatcg
     4201 aagcgtaccg cgcatcacag ggacccgctg ccggtagcgg cttcccctgg ggcacggtac
     4261 tgatgatcgc gcttgcactg gtggtggtcg cgggggtgta cctcctggcc acgcgcgccg
     4321 ccatgcgcac gcgcgccatc gacctgttgc gtggtttcgc ggagggactg aaagccatcc
     4381 tcagcaccag acgaaaggct gcttttctgg gccataccct cttcatctgg gctgcctacc
     4441 tcgccatgtt cgtcatcggt ttccagtgcc tgcccagcat gaaagacgta cccgtggcgg
     4501 gcatcctcgc gggcttcatc gccggtgcca tcggcatcgt gctggtgcag ggcggcatcg
     4561 gcgtgtaccc cgcgttcgtg gccctcatcg tgggggtgta catggccccg ccagcgggcg
     4621 gcgggctgct gcgccccgat gccctggcca tgggctggct
//
LOCUS       DAOQOC010000074         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7930_length_4653_cov_3.489996, whole genome shotgun sequence.
ACCESSION   DAOQOC010000074 DAOQOC010000000
VERSION     DAOQOC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7930_length_4653_cov_3.489996"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="kdsA"
                     /locus_tag="PL106_01985"
     CDS             complement(<1..557)
                     /gene="kdsA"
                     /locus_tag="PL106_01985"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNA31867.1"
                     /translation="MKDTRIVHVGGIACGSEQLFLISGPCVIESDEVMMRTAEKLKEV
                     SERLRIPVIYKSSFTKDNRSSVDFYQGPGLEEGLRSLQRIKQEFGFPLLTDIHNPQQA
                     APVAEVCDVLQIPAYLCMQTSLILEAAKTGAVINIKHGQFLAPDNMIKPVEKARSVGN
                     DKIILTERGYTFGYNDLVVDPRAFYL"
     gene            complement(669..1283)
                     /locus_tag="PL106_01990"
     CDS             complement(669..1283)
                     /locus_tag="PL106_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNA31868.1"
                     /translation="MKVICIGRNYRDHAVELGNEVPTEPVFFLKPTTACLYPGQIIRM
                     KPHLGEIHHELELVVMVDSYASNVPTSMALGLVASFTLGLDLTARTVQNELKARGLPW
                     EKAKAFDGSAVLGEVELPMTAGTDLQGYEIQLKRNGNLVQQGHTRDMIFTVADIIAHV
                     SRYITLEPGDLIFTGTPAGVGPIEHGDLLEGFLNGNCVLRVDVE"
     gene            complement(1413..2183)
                     /locus_tag="PL106_01995"
     CDS             complement(1413..2183)
                     /locus_tag="PL106_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNA31869.1"
                     /translation="MLLRLERPLAVFDLETTGTRIGRDRIVQIAIVRLLPGGERERYQ
                     SLVNPGIPIPPEATAIHGISDRDVATAPRLDDIAGEVLGHLAGCDLAGFNAIRFDVPF
                     LTEELFRTGHAWDTAQLRIVDAQRIFHKKEPRDLSAALKFYTGREHAMAHDALGDVEA
                     TADVLLAQLERYPDLPHDVDALGAFSGDRDRSPDAAGKLAFDDRGQVCLSFGKYKGWT
                     LENIGRNDPGYLQWLMSKAELPGSTLAVMRAVLADLQD"
     gene            complement(2253..4448)
                     /locus_tag="PL106_02000"
     CDS             complement(2253..4448)
                     /locus_tag="PL106_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31870.1"
                     /translation="MRNGHAALTTVLFFSFTLGASAQGENTSFQWKFEEACKLNEERF
                     HNQAADIWKQLVEAQPENSNLNWKLGSSYMNSYNQKAKALPYLQTASDRRGKGNGAFN
                     ATGYDPFDSKETNAPVEVDYWLAYAYHLAGNFAKADEYYTKFSTEVGDKHFLYQSAQH
                     GLEQTANARKLIAEPKTYTVSNAGPVINNDHPDFSPVISVDGNAMFYTSRRLRADSSN
                     VDIVDPLAGLPFENIYVSYKDRNANWQTPEPLNINAVDRHFATVNVSADGQTLYIYRD
                     DGGDGNLYESKLVGELWSDPVLIGSDVNTKAWETHCALTADGNTLYFVSDRKGGEGGR
                     DIYRVVKLPTGEWSKAQNMGKVLNTIYDEDGVFIHPNGRTLYFASKGHNSMGGFDIFT
                     SELKDDGTWSVPENIGYPLNTVDDDVFFVTTADGRRGYFSSDKAGGYGEKDIYFVDFP
                     ESMEAEGLTVLKGFIIPPPGESLPPSTILYVTDKSTGEVKTYKPRQRDGVYVAILPPC
                     KEYNLDYRVNDKTVHTEDIFVECESTYQEINKEIYLNPVSLAGPASIVDLPKGAPPGS
                     KEKGVAPDQVGKTKGKDAVKEVPLTDKEIATKVRTHVPPDAKYSDEYAKYYAYNIKDI
                     DQGEAEWKKFIDSVVGILDKDGTARIVIEGSASRVPTKTFGTNDNLSNQRMEDARKRL
                     LEAVKARGKDETKLLLEAVNHLVQGPKYVGDYQNTEKYGKYQYVKLKVR"
BASE COUNT          801 a         1409 c         1398 g         1045 t
ORIGIN      
        1 aggtagaagg cccgtggatc caccaccagg tcgttgtatc cgaaggtgta cccacgctcg
       61 gtaaggatga tcttgtcgtt gcccacgctc ctggctttct cgaccggctt gatcatgttg
      121 tccggtgcga ggaactgccc atgcttgatg ttgatcaccg caccggtctt cgcggcctcc
      181 aggatcaacg aggtctgcat gcagaggtac gcggggatct gcagaacatc acacacctcg
      241 gctacaggcg ccgcctgctg cgggttgtgg atgtcggtaa gcaacgggaa accgaactcc
      301 tgcttgatgc gctgaagtga cctcagccct tcttccaagc cgggaccctg gtagaaatcg
      361 acgctgctgc ggttgtcctt ggtgaagctg ctcttgtaga tgaccgggat cctgaggcgc
      421 tcgctcacct ccttcagctt ctcggcggta cgcatcatca cctcatcgct ctcgatcacg
      481 caaggaccgc tgataaggaa gagttgctcg ctgccgcagg cgatcccgcc gacgtggacg
      541 atccgggtgt ctttcactgg tgaacggttc aagaggccgc aaagctatcc tttggagccg
      601 ggatccggtg ccggaacagg tcaagccgtt ccggacaggc cgctggctcg ctgactttca
      661 tgcgctcctt actccacatc cacacggagc acgcagttgc cgttcaggaa accctccagc
      721 aggtcgccgt gctcaatggg ccccacccct gccggtgtgc ccgtgaagat gaggtcgccc
      781 ggttccaagg tgatgtagcg cgacacatgg gcgatgatat cggccaccgt gaagatcatg
      841 tcccgcgtat ggccctgctg tacgaggttg ccgttgcgct tgagttggat ctcgtagccc
      901 tgcagatcgg tgccggccgt catgggcagc tccacctcgc ccaacacagc actgccatcg
      961 aaggctttcg ctttttccca tggcaggccc ctcgccttca gctcattctg caccgtacgt
     1021 gcggtgaggt cgagccctag ggtgaaggaa gccacgaggc ccaaggccat ggaagtgggc
     1081 acattgctgg cgtagctgtc gaccatgacc accagttcca attcgtgatg gatctcgccg
     1141 agatgcggct tcatccggat gatctgtccc ggatacagac aggccgtggt gggcttcaga
     1201 aagaataccg gttccgtagg cacctcattc ccgagttcca cggcgtggtc gcggtaattc
     1261 cgcccgatgc agatcacctt catgcctcta agttatacca tcccgacatc cgttggtcgg
     1321 gacttttccg atcggaccgc cacatcgcac ggacccatgg taccgacccc tggcatgcga
     1381 ggggtgtatg tatgcctcat gtgcggtatc attcaatcct ggaggtcggc cagcaccgcc
     1441 ctcatcacgg ccagggtact gcccggcagt tcggccttgg acatgagcca ttgcaagtaa
     1501 ccgggatcat tgcgcccgat gttctcgagt gtccagcctt tgtatttccc gaagctcagg
     1561 cacacctggc cacgatcgtc gaaggcgagt ttgcctgccg catcggggct gcggtcgcga
     1621 tcgccgctga acgcacccag cgcatccacg tcgtgcggga gatccggata acgctccagt
     1681 tgtgcgagaa gaacgtcggc cgtggcctcc acatcgccca gcgcatcgtg cgccatggcg
     1741 tgttcacgtc ccgtgtagaa cttgagcgcg gcgctcagat cgcgtggttc cttcttgtgg
     1801 aagatccgtt gggcatccac gatccgtagt tgcgcggtgt cccacgcgtg gcccgtgcgg
     1861 aacagttctt cggtgaggaa gggcacgtcg aaccggatgg cgttgaagcc cgcgagatcg
     1921 caacccgcga ggtggcccaa cacctcaccg gcgatgtcgt ccaagcgggg tgcggtcgcc
     1981 acgtcccgat cgctgatccc atggatggcg gtggcctcgg gcgggatggg gatccccggg
     2041 ttcaccaaac tctggtagcg ttcgcgttcg ccaccgggca gcaggcgcac gatggcgatc
     2101 tgcacgatgc ggtcgcgtcc gatgcgtgtg cccgtcgtct cgaggtcgaa gacggcgagc
     2161 ggtcgttcaa ggcggaggag catgcgtgaa aggtcgggaa gagcaagccc cgtccggcgg
     2221 tccggacggg gcttcgtttt caacagattg gatcagcgga ccttcagctt cacgtactgg
     2281 tacttgccgt acttctccgt gttctgataa tcacccacgt acttgggtcc ctgcaccagg
     2341 tggttcacgg cctccagcag cagcttggtc tcgtccttgc cgcgtgcctt caccgcttcc
     2401 agcaggcgct tgcgcgcgtc ctccatgcgc tggttgctca ggttgtcgtt ggtgccgaag
     2461 gtcttggtgg gcacacggct ggcgctgcct tcgatcacga tccgtgccgt gccgtccttg
     2521 tccaggatac cgacgaccga atcgatgaat ttcttccact ccgcctcgcc ctggtcgatg
     2581 tccttgatgt tgtacgcgta atacttggcg tactcgtcgg agtatttcgc atccggcgga
     2641 acgtgcgtcc tgaccttggt ggcgatctcc ttgtcggtaa gcggaacctc cttgaccgcg
     2701 tccttgccct tggtcttgcc gacctggtca ggagccacgc ccttctcctt gcttccggga
     2761 ggtgcaccct tgggcaggtc gacgatgctg gcgggaccag cgagcgacac ggggttgagg
     2821 tagatctcct tgttgatctc ctgataggtg ctctcacact ccacgaagat gtcctccgtg
     2881 tgtaccgtct tgtcgttcac ccggtaatcc aggttgtact ccttgcatgg cggcagaatg
     2941 gccacgtaca cgccatcccg ttggcggggc ttgtaggtct tcacctcgcc ggtgctcttg
     3001 tcggtcacat aaaggatggt gcttggcggt agcgactcgc caggcggggg gatgatgaag
     3061 cctttgagca ccgtgagtcc ttcggcctcc atgctctccg ggaagtccac gaagtagatg
     3121 tccttctcgc catagccccc ggccttgtcg ctgctgaaat acccacggcg gccatcagcg
     3181 gtggtcacga agaaaacatc gtcatccacg gtgttcaacg ggtaaccgat gttctcgggc
     3241 acgctccacg taccatcgtc cttcagctcc gaggtgaaga tatcgaagcc gcccatgctg
     3301 ttgtggccct tgctggcgaa gtacagggtg cggccgttgg ggtggatgaa tacgccgtcc
     3361 tcatcgtaaa tggtattgag cactttgccc atgttctggg ccttgctcca ttcgccggtg
     3421 ggcagtttca ccacgcggta aatatcccgt ccgccctcgc ctcctttccg gtcactcacg
     3481 aagtaaagcg tgttgccatc ggcggtgagc gcgcagtgcg tttcccaggc cttggtgttc
     3541 acatcactcc cgatgagcac cgggtcactc caaagttcac ccacgagttt gctttcgtac
     3601 aggttgccat cgccgccgtc atcgcgatag atgtacagtg tctgaccatc ggcgctcacg
     3661 ttcaccgtgg cgaagtgccg gtcgacggcg ttgatgttca gtggttcagg cgtctgccag
     3721 ttggcgttgc ggtccttgta gctgacatag atgttctcga agggcaggcc cgcgagcggg
     3781 tctacgatgt ccacgttgct gctgtcggca cgcaggcggc ggctggtgta gaacatcgcg
     3841 ttcccatcca cgctgatcac cgggctgaaa tccgggtggt cgttgttgat cacggggccg
     3901 gcgttgctca ccgtataggt cttgggctcg gcgatcagtt tgcgcgcgtt ggccgtctgt
     3961 tccagtccgt gctgggccga ctggtacagg aagtgcttgt cgcccacctc ggttgagaat
     4021 ttggtgtagt actcatcggc cttggcgaag ttccctgcca ggtgataggc gtaggccagc
     4081 cagtaatcca cctccactgg tgcgttggtc tccttgctgt cgaaggggtc gtacccggtc
     4141 gcgttgaaag caccgttgcc cttgccacga cggtccgatg cggtttgcaa atagggcagc
     4201 gccttcgcct tctggttgta cgaattcatg tacgaggagc ccagcttcca gttcaggtta
     4261 ctgttctcgg gctgtgcttc cacaagctgc ttccagatgt cggctgcctg gttatggaac
     4321 ctctcctcat tcagcttgca ggcctcctcg aatttccatt ggaaagatgt gttctcccct
     4381 tgggcagagg ccccgagcgt gaaggaaaag aacaagacgg tcgttagtgc ggcgtgacca
     4441 ttcctcattg tagagcgatt tcgatccact ggtggcggac ggtggcgaaa gtagcctttt
     4501 tccgaaggga ggaagcctgt tcaaaactcg ccatccacca tgcgttgtgg atcgctcgga
     4561 cgactccgcg aacggctcgg gatcagaact cctgatcgat ggaccagccc tcgagaatgt
     4621 cggccacacg gcggacgaac cttccaccca agg
//
LOCUS       DAOQOC010000075         4638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_7970_length_4638_cov_4.118700, whole genome shotgun sequence.
ACCESSION   DAOQOC010000075 DAOQOC010000000
VERSION     DAOQOC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7970_length_4638_cov_4.118700"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..529)
                     /locus_tag="PL106_02005"
     CDS             complement(56..529)
                     /locus_tag="PL106_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA31871.1"
                     /translation="MQLSIIVPLYNEETLIVDLLDKLGRVRWPSFVAGVEIVVVDDAS
                     TDRSHEVMMRYVQSRTGIRFHRQDRNRGKGAAVRKGAELATGDHLLVQDADLELDPND
                     IPSLLEAMRELGVPFVNGSRYLPGVRRTQASYKRYFANKLLPATLKDDTTNKNEQ"
     gene            complement(595..2433)
                     /locus_tag="PL106_02010"
     CDS             complement(595..2433)
                     /locus_tag="PL106_02010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA31872.1"
                     /translation="MPPRSRAFPISLAALLLVWLLLAFGIFHDPGRTRFTWDSFGYHL
                     YLPATVIHHDPLLKDLGWVDQARKDHDASGTLYQISTLEDGSHVDKYTMGLAVLWSPW
                     FMAGHAIAKATGQPQDGYSPPYDLAVRIGVWCYLLLGMMALRAVLRERFGDRVAAIAL
                     LLLFAATNLIDQATLSTAMPHITLFSLYAGMLWATLRWAARRRVGWALILAVLMGLAM
                     LVRPSEGVCVLLPFLWRGEKEVAGPFIRAWRLRRQWLSIIVVMFLVGLPQFIYWKAAT
                     GHWFLDTYNNAGEGFDLFSPHTWPFLFSFRKGWYIYTPLMLLATAGIPLLRKYWHEAF
                     QPVLVFFILNLYLVSSWTNWWYADSFGSRAMVGSYAVMALPLAAVLQWAGDGASVRRW
                     AAGLVITVLTALNLFQYWQYGQGIIHSSRMTMAAYGSTFGRTTIPSNMEDLLLVYRSY
                     GSDQGRPDSTRYERIPMPELSTLLPAPDTIFRDTSTGILNKAFRLEGQHPFTPALRVA
                     FHDLTRNDHAWVEAEWRVWLSDTAAKGSFVNTFEHGGKNYAYQAEDIGMKHLPAGQWV
                     TLSTWYLTPEVRSGEDLFVAYFWLRDTLPMYVSGPMIILHRPRSSD"
     gene            2901..>4638
                     /locus_tag="PL106_02015"
     CDS             2901..>4638
                     /locus_tag="PL106_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31873.1"
                     /translation="MMRTKGPSVDRGGELLGVLVAIALFIAGLWSVPLALFGPDRTLI
                     PGDMGDARFNNWILEHFHQYVTGKVGSFWDAPFMYPWKNAIALSDNLLGSAPIYSALR
                     YLGQTREGAFQGWIVTMFALNYGCCFVAMRKWAGNSALAACASYIFAFGIYNIGQLNN
                     LQVLPRYMAPLALVSLWSHLHSGSWKALVLAMCATVYQFYCGIYIGFILVYALFFLLV
                     GHLLIFRDPGFLKRFTRWRFTGIWFGTVLIGLLMLAPMIKHYLAVPEELGVRTYADIF
                     ETIPRPSSYFFTHPAAMSWRSLYTIAENAFPQWWYHLHFLGALPWLAVLGTPFMLLSK
                     RVTLVERRTMAAAVTALVLSTLFCLNIGGFSLYKLVFMLPGFSVLRAIDRFINVQVLF
                     FLIVFVLVLRPLFQRRRTAWALSLLLPVMVVQDNRWEVGYIDRYDKHVSERVVADVER
                     RIARELDRSGQHDAVAYEPALPIIRDFEEWHTLVITTQINAMLAGQELGIPVVNAYSG
                     GYPGNYIKFFDAMDHGTMENWCAFNGIGTERIQEIDGLAVDITARDTVRIIAANGLYL
                     CADRTRGNTLLAS"
BASE COUNT          916 a         1318 c         1433 g          971 t
ORIGIN      
        1 gcgttgggtt gcagctcttt ttattttgcg aagggttggc attttgtctg tcgtattact
       61 gttcgttttt atttgtcgtg tcgtctttta gggttgctgg taacagcttg ttggcgaaat
      121 agcgcttgta agaagcttga gtgcgtctta cgcctggcaa gtagcgtgag ccgttcacga
      181 aggggacccc cagctcgcgc atcgcctcca gtagtgaggg gatgtcattc gggtccagct
      241 ccaggtccgc atcctgcacc agcaaatgat cgcccgtggc aagctcggca cctttgcgca
      301 ccgcagcacc cttgccgcga ttgcggtcct gtcgatgaaa tctgatcccc gtcctgctct
      361 gcacataccg catcatcacc tcatgggagc ggtcggtcga cgcatcatcc accacgacga
      421 tctccacacc agccacgaaa gacggccacc gaacccggcc aagtttgtcc aacaggtcga
      481 cgatcagggt ctcctcgttg tacaaaggaa caataatgct gagttgcatg atgctttatg
      541 cggtgcgcga agtaatcagg gaggcggggt tgatggttga aggtggccgc cgggtcaatc
      601 cgaactcctg ggccggtgca ggatgatcat cgggcccgat acatacatcg gcagtgtgtc
      661 gcgcaaccag aaataggcta cgaacaggtc ttccccgcta cggacctcgg gcgtcaggta
      721 ccatgtgctc aaggtgaccc attgcccggc cgggaggtgc ttcatcccga tgtcctcggc
      781 ctggtacgca tagttcttgc cgccgtgttc gaaggtgttg acgaagctgc ctttggcggc
      841 cgtgtccgac agccacacgc gccactcggc ctccacccag gcgtggtcgt tccgggtcag
      901 atcatggaat gccacacgga gtgctggtgt gaacggatgt tgtccctcca ggcggaaggc
      961 cttgttcagt atgcctgtgg acgtatcgcg gaagatcgtg tccggggcag gcagcagggt
     1021 ggaaagctcc ggcatgggga tccgttcgta cctggtgctg tccggccgcc cttgatcgct
     1081 gccataggag cggtacacca ggagcagatc ctccatgttg ctcgggatcg ttgttcttcc
     1141 gaaggtggag ccgtaggccg ccatggtcat tcgtgaggag tggatgatcc cctgtccgta
     1201 ttgccagtac tggaacaggt tgagcgcggt caggacggtg atgaccaggc ccgctgccca
     1261 ccgccgaacg gatgcgccat cacccgccca ttgcagcacg gcggccaagg gaagggccat
     1321 caccgcgtat gaccccacca tggcgcgcga cccgaagctg tcagcatacc accagttggt
     1381 ccaactggaa accaggtaca agttgaggat gaagaagacc agaacgggtt ggaatgcttc
     1441 atgccaatac ttgcgcagga gggggatgcc ggcagtggcc agcagcatca gcggtgtata
     1501 aatgtaccaa cccttgcgga agctgaacag gaacggccat gtgtgtggag agaatagatc
     1561 gaagccttcc ccggcgttgt tgtacgtgtc caggaaccag tggccggtgg cggccttcca
     1621 gtagatgaat tgcggaaggc cgaccaggaa catcacgacg atgatggaca gccattgcct
     1681 gcgcaaccgc cacgcacgga tgaacggtcc cgccacttcc ttttcgccgc gccacaggaa
     1741 gggcagcaac acgcatacgc cttcgctggg ccgaaccagc atggccaagc ccatcagaac
     1801 ggcgaggatc aaggcccatc caacgcgccg tcgcgcagcc cagcgcaggg tggcccacag
     1861 catgccggca tagagcgaga acagcgtgat gtgcggcatg gccgtgctca acgtggcctg
     1921 gtcgatgagg ttggtggcgg cgaagagcag cagtagtgca atagccgcga cccgatcacc
     1981 aaagcgttcg cgcaacaccg cacggagtgc catcatcccg agcagcaggt aacaccacac
     2041 cccgatccgc acggccaggt cataaggtgg cgaatagccg tcctgcggct ggccggtggc
     2101 tttggcgatc gcgtgcccag ccatgaacca cgggctccac agaaccgcga gccccatggt
     2161 gtacttgtcc acgtggctgc catcttccaa tgtgctgatc tggtacagcg tgccggaggc
     2221 atcgtggtcc ttgcgtgcct ggtccaccca gccgaggtcc ttcagcagtg gatcatggtg
     2281 aatgaccgtg gccggcaaat agaggtggta gccaaaggag tcccatgtga aacgggtgcg
     2341 gccggggtcg tggaagatgc cgaaagcgag caggagccag accaggagca gagccgcaag
     2401 ggagatggga aaggctcgtg agcgtggtgg cattatgacg gatccggaaa atgtaaccca
     2461 atggtcaatg ccgtggccat tatgaatgca agcagtggga gaaggaaaag gccggtatgc
     2521 gttcgcagca aaggcatgtt gaaaagcaat tgctgacaaa agacggaagg cgagcagcca
     2581 cctgcgaccg gcatttcaaa tagcatgagc gccactctgt ttgatcagca atcccgcatc
     2641 gctttcattc atcctgactt gatcgatgcc tgaagtcggg ttgttctgga tgacacccct
     2701 ggcggctctc cgtcaaggga tcgcattggg ccgaaaggta gcaggaggct gtacgttacc
     2761 acattgaacg cttcgttcaa tcctcctcgg gagggtcgat ggatgaccct gtatttgtgg
     2821 cccctgtccc atccatggat catcgctgat ccgccgctgt ggtgagtgtg ggaaggtgtg
     2881 ggtaccattg cagccggaca atgatgcgga ccaagggacc ctcggtggac agaggcggcg
     2941 aactgctggg tgtactggtg gccattgccc tgttcatcgc aggcctctgg tccgtgccgt
     3001 tggcgctttt cgggcccgat cgcacgttga tccccggcga tatgggcgac gcccgtttca
     3061 acaactggat cctggagcat ttccatcagt atgttaccgg aaaggtgggg agtttctggg
     3121 atgccccgtt catgtacccg tggaagaacg ccattgcctt gagcgacaac ctgctcggca
     3181 gtgcgccgat ctattccgca ttgcggtact tgggccagac ccgcgaaggg gcgttccagg
     3241 gctggatagt caccatgttc gccctgaact acgggtgctg tttcgtcgcc atgcggaaat
     3301 gggcgggaaa ttcggcactc gcggcatgcg catcgtacat cttcgctttc ggcatataca
     3361 acatcgggca gctcaacaac ctgcaggtgt tgccccgcta catggcgcca ttggccctcg
     3421 tgtcgctgtg gagccacctg cactccgggt cctggaaggc gttggtgctg gccatgtgcg
     3481 ccaccgttta ccagttctac tgcggcatct acatcggctt catcctggtg tacgctctgt
     3541 tcttcctcct ggtcggccac ttgctcattt tccgggatcc cggtttcctg aagcggttca
     3601 cgcgttggcg gttcaccggt atctggttcg gtacagtgct catagggtta ctgatgctgg
     3661 cgccgatgat caaacattat ttggccgtgc ccgaagaatt gggtgtgcgg acctacgccg
     3721 acattttcga gaccatcccg cggccatcct catatttctt cactcatccc gcagcgatgt
     3781 cctggcgatc gttgtacacg atcgcggaga atgcctttcc acaatggtgg taccacttgc
     3841 acttcctcgg ggcactacct tggctggcgg tgctggggac tccgttcatg cttctgtcca
     3901 agcgcgtgac cttggtggaa cgccggacga tggcggcggc tgttacggcc ttggtgctca
     3961 gtacgctgtt ctgtttgaac atcggcggct tcagcctgta caagctggtg ttcatgctgc
     4021 ccggcttttc cgtgctgcgc gccatcgatc gcttcatcaa cgtgcaggta ttgttcttcc
     4081 tcatcgtgtt cgttctggta ctgcgtcccc tgttccaacg gcgcaggacc gcatgggcgc
     4141 tcagcctgct actaccggtg atggtggtgc aggacaaccg atgggaagtc gggtacatcg
     4201 atcggtacga caagcacgtt tcggaacgcg tggtggcgga cgtggaacgg cgaattgcac
     4261 gggagctgga ccggagcggc caacatgatg cggtcgcgta cgagccggca ttgccgatca
     4321 tcagggattt tgaagaatgg catacgctcg tgatcaccac gcagatcaac gccatgctgg
     4381 ccgggcaaga gctggggatc ccagtggtca atgcatacag tgggggctac cccggcaact
     4441 acatcaaatt cttcgatgcc atggaccatg gcaccatgga gaattggtgc gcattcaacg
     4501 ggatcggcac ggagcggatc caggagatcg acggtctggc agtggatatt acggcccggg
     4561 ataccgttcg catcatcgcg gccaacgggc tgtacctctg cgcggaccgg acgcggggga
     4621 acaccctcct ggcgagtc
//
LOCUS       DAOQOC010000076         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8112_length_4597_cov_3.785777, whole genome shotgun sequence.
ACCESSION   DAOQOC010000076 DAOQOC010000000
VERSION     DAOQOC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8112_length_4597_cov_3.785777"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..500)
                     /locus_tag="PL106_02020"
     CDS             complement(36..500)
                     /locus_tag="PL106_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNA31874.1"
                     /translation="MSQERLSAFNDLCMEEPNEPYGSISSYRDLVVWQHSMDVAEMVY
                     EFAALLPNEERFGLMSQMCRASVSMPANIAEGWGRGTSSDRQLLSYLRITRGSLYELE
                     THLELARRLFRKLQLDPGPITSRLTSIARMLNKMIFKIEARYESMRKERGGR"
     gene            complement(552..2567)
                     /locus_tag="PL106_02025"
     CDS             complement(552..2567)
                     /locus_tag="PL106_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNA31875.1"
                     /translation="MSTLDSKIPPGPIDKKWTYHKGHARIVNPANKRRIDVIVVGTGL
                     AGGAAAASLAEQGYSVKAFCFQDSARRAHSIAAQGGINAAKNYQNDGDSTYRLFYDTV
                     KGGDYRSREANVYRLAEVSANIIDQCVAQGVPFARDYGGLLDNRSFGGVQVSRTFYAR
                     GQTGQQLLLGAYSALMRQVGKGAVQMFDRHEMLDVVIVDGKARGIIARNLITGEIERH
                     GAHAVLLCTGGYGNVFFLSTNAMGSNVTAAWKAHKRGAYFGNPCYTQIHPTCIPVSGT
                     HQSKLTLMSESLRNDGRIWVPAKLEDAKAIREGKKKGRDIPEADRDYYLERRYPAFGN
                     LVPRDVASRAAKERCDAGFGVNRTGEAVYLDFNAAFERYGKQQANVLKIDNPSKEKII
                     ELGKAVVSEKYGNLFDMYENIVGENPYEHAMKIYPAVHYTMGGLWVDYNLQTTVPGLF
                     CLGEANFSDHGANRLGASALMQGLADGYFVIPYTVSDYLADDIRSGPIDTKRPEFVAA
                     EKEQVDRINHFLNNKGTKPVDDFHRALGKVMWDKCGMARNAQGLQEAIQEIRAIRDDF
                     WKNVRVPGKAGEFNQELEKAGRVADFLELGELMCMDALNRNESCGGHFREEYQELDGP
                     QKGEAKRDDANYAYVAAWEWLGEAGKAQLHKEALTFEEVKLTQRSYK"
     gene            complement(2680..3363)
                     /locus_tag="PL106_02030"
     CDS             complement(2680..3363)
                     /locus_tag="PL106_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNA31876.1"
                     /translation="MARTGLFGSSLVKKYWMALTGLFLISFLIVHCAINALIFFNDGG
                     VTFNTGAEFMAKNPIIRAMEIVLFIGLIAHVVDGLMVWRENRAARPVKYVMEKGSASR
                     TWYSASMGILGTLILLFLILHLWHFWVKSRITGLHDYGVDAEGRENLYAVMVEVFQNP
                     VAVIIYVLGCFSLFWHLLHGFKSAFQSLGINHKRYNGFIAACGTAFSIVVPTLFAIMP
                     VSIFLQWIQ"
     gene            complement(3587..>4597)
                     /locus_tag="PL106_02035"
     CDS             complement(3587..>4597)
                     /locus_tag="PL106_02035"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNA31877.1"
                     /translation="EGDEACDDGNTDDTDACSNDCTAAFCGDGVVQPALEQCDDGDMD
                     DTDSCVGDCKLATCGDGFVHAGVEACDDGPNNDDAACRFLSMWCPPPYMAGCSQVAWT
                     KGAPTLIRNYDFDPRYFDGRMRFTEYVKPVIGIQDSGWGLLDGMNIDGLAVALAFGGR
                     KVSGTGFGIPLLIRYVLETCSTTDEACETLSRVPVHMGYNVTVVDKSGKYATVYLNPD
                     RPPQVIYQAVACNHQHQVEWDEYAAFTNTIERKHYLEHSIAQPRLTRAALIKQFLKPP
                     LYSQQYLRGFGTLYTAAYDLSKGRVQIIWPEKQVEASFSRFEEQDVQVVLLRPVGRYL
                     AK"
BASE COUNT          795 a         1410 c         1435 g          957 t
ORIGIN      
        1 aatggtcaat tggtgaatgg cgaatgggga agccctcatc ggccaccacg ttccttgcgc
       61 attgattcgt accgcgcctc gatcttgaag atcatcttgt tcaacatccg cgcgatcgag
      121 gtgaggcgcg atgtgatcgg tcctggatcc aactgaagct tgcggaatag cctgcgggca
      181 agctccaggt gcgtttcgag ttcgtacaat gacccgcggg tgatccggag ataactcagc
      241 aactggcggt cactgcttgt tcctcgtccc cagccctcgg caatgttcgc aggcatggaa
      301 accgaagcac ggcacatctg actcatcaat ccgaagcgct cttcattcgg aagtagcgcg
      361 gcgaactcgt aaaccatttc tgcgacgtcc atggaatgtt gccaaacgac aaggtcacga
      421 tagctgctga tcgacccata aggctcgttc ggctcctcca tgcagaggtc attgaaagca
      481 ctcagtcgtt cctggctcat ccctttcagt tcctggcatt cccattcacc aattgaccat
      541 tcgccattca cctacttgta actcctctgc gtcagcttca cctcctcgaa cgtcagtgcc
      601 tccttgtgca gttgggcttt acccgcctcg cccagccatt cccaggccgc cacgtaggcg
      661 tagttcgcat cgtcgcgttt cgcttcgccc ttctgcgggc cgtccagctc ctggtattcc
      721 tccctgaagt ggcccccaca gctctcgttg cggttcagtg cgtccatgca catcagttcg
      781 cccagttcca ggaagtcggc cacacgaccg gccttctcca gctcctggtt gaattccccc
      841 gccttgccgg gcacgcgcac gttcttccag aaatcatccc ggatcgcacg gatctcctgg
      901 atcgcctcct gcaacccctg cgcattgcgc gccatgccgc acttgtccca catcaccttg
      961 cccagtgcgc ggtggaagtc gtccacgggc ttcgttccct tgttgttcag gaagtggttg
     1021 atccggtcca cctgctcctt ctcggcggca acgaactccg gtcgcttggt gtcgatgggt
     1081 ccggagcgga tgtcgtcggc caggtaatcg ctcaccgtat aggggatcac gaagtagcca
     1141 tcggccaggc cctgcatcag ggctgaggcc ccgaggcggt tggcgccgtg atcgctgaag
     1201 ttggcctcac ccaggcagaa gaggcccggc accgtggtct gcaggttgta atccacccac
     1261 aggccaccca tggtgtagtg tacggcgggg tagatcttca tcgcgtgctc gtacgggttc
     1321 tcgcccacga tgttctcgta catgtcgaag aggttgccgt acttctcgct caccacggcc
     1381 ttgcccaact cgatgatctt ctctttgctc gggttgtcga tcttcagcac gttcgcctgc
     1441 tgtttgccgt agcgctcgaa ggccgcgttg aagtccaggt acacggcctc gccggtcctg
     1501 ttcacgccga agcccgcatc gcagcgctcc ttggcggcac ggctggccac gtcgcgcggc
     1561 accaggttac cgaaggccgg gtagcggcgt tccaggtaat aatcgcggtc ggcctcgggg
     1621 atgtcgcggc ctttcttttt gccttcgcgg atcgctttcg catcctcgag cttggcgggc
     1681 acccagatgc ggccgtcgtt gcgcaacgat tcggacatca gggtgagttt gctctggtgc
     1741 gtgccgctca ccgggatgca ggtggggtgg atctgcgtgt agcaaggatt gccgaagtag
     1801 gcaccgcgct tgtgcgcttt ccaggccgcg gtaacgttgc tgcccatggc gttcgtgctc
     1861 aggaagaaca cgttgccgta cccgccggta cagagcagca cggcatgtgc accatggcgt
     1921 tcgatctcgc cggtgatcag gttgcgggcg atgatgccgc gtgccttgcc atcgacgatg
     1981 accacatcca acatttcgtg gcggtcgaac atctgcacgg cgcctttgcc cacctggcgc
     2041 atcagtgcgc tgtaggctcc cagcagcaat tgctgaccgg tctgcccgcg ggcgtagaag
     2101 gtgcggctca cctgcacacc gccgaagctg cggttgtcga gcagtccacc atagtcgcgg
     2161 gcgaagggca cgccttgggc cacgcactgg tcgatgatgt tggcgctcac ttccgccaag
     2221 cggtacacgt tggcctcgcg cgagcggtaa tcacctcctt tcacggtatc gtagaacagc
     2281 cggtaggtgc tgtccccgtc gttctggtag ttcttcgcgg cgttgatacc tccttgtgcg
     2341 gcgatgctgt gcgcacgccg cgcgctgtcc tggaagcaga aggccttcac gctgtagccc
     2401 tgttcggcga gcgatgcggc ggcggcgcca ccggccaggc ccgtgcccac cacgatcacg
     2461 tcgatgcggc gcttgttcgc cggattcacg atgcgggcgt gccccttatg gtaggtccac
     2521 ttcttgtcga tggggccggg cgggatcttg ctgtcgaggg tgctcatggg aggagggaag
     2581 ggcgaatggt caatggcgaa tagcgaatgg agaaagtcgg tctggcccgt tcgccattca
     2641 ccaactccca ttcacctgtg ttgggcatat gcgcggacat cactggatcc actggaggaa
     2701 gatggacaca ggcatgatcg cgaaaagggt gggcaccacg atgctgaatg ccgtgccgca
     2761 ggcggcgatg aagccgttgt agcgtttgtg attgatgccg aggctttgga aggcgctctt
     2821 gaagccgtgc aacaggtgcc agaagaggct gaagcacccc aggacgtata tgatgacggc
     2881 cacgggattc tggaacacct cgaccatcac ggcatagagg ttctcccgtc cttcggcatc
     2941 cacgccgtag tcgtgcaggc cggtgatccg gctcttcacc cagaagtgcc agaggtgcag
     3001 gatgaggaag agcaggataa gcgtgccgag gatccccatg ctggcgctgt accaggtgcg
     3061 gctggcgctg cctttctcca tgacgtactt caccgggcgt gcagcgcggt tctcgcgcca
     3121 gaccatcagg ccgtccacca cgtgggcgat gaggccgatg aagagcacga tctccatggc
     3181 gcggatgatc ggattcttgg ccatgaactc ggcaccggtg ttgaaggtga ccccgccgtc
     3241 gttgaagaag atgagcgcat tgatggcgca gtggacgatc aggaaggaga taaggaaaag
     3301 gcccgtgagg gccatccaat acttcttgac gagggaggaa ccgaagagtc ctgtgcgggc
     3361 catgtgagcg gaatgggaat ggccgcgaat ttacgggctg ttccgggatg aaagacctcg
     3421 gaagcggtcg gcttgtccag aactgttctg atcggaccgc aggtcattcg gtagagccac
     3481 gctccgtcag cgatgaacga caggccgtgc gtgttcgcga ccgatcgcgg tcacgggtcc
     3541 acacccggag gcagtgtcca catgatccgg atgccacgta cgagcactac ttcgcaaggt
     3601 acctgcccac cggtcgcaag agcaccacct gcacatcctg ttcctcgaag cgcgaaaagc
     3661 tcgcctctac ctgtttctcg ggccagatga tctgcacgcg ccccttggac aggtcgtagg
     3721 cggcggtgta caaggtgccg aagccgcgca ggtattgctg gctgtacaag ggcggcttca
     3781 ggaattgctt gatgagcgcc gcccgtgtga gtctgggctg cgcgatgctg tgctccaggt
     3841 aatgcttgcg ttcgatggtg ttggtgaatg cggcgtactc gtcccactcc acctggtgct
     3901 gatggttgca cgccacggcc tggtagatca cctggggcgg ccggtcggga ttcagataga
     3961 cggtggcgta ctttccgctc ttgtccacca cggtgacgtt gtagcccatg tgtaccggga
     4021 cgcgcgagag ggtctcgcat gcctcatcgg tcgtgctgca cgtttcgagg acgtaccgga
     4081 tgagcagcgg gatgccgaaa cccgtgccgc tcaccttccg gccgccgaaa gcgagggcca
     4141 cggccaaccc gtcgatgttc atgccatcga gcaggcccca cccgctgtcc tggatcccga
     4201 tcaccggttt cacgtattcg gtgaagcgca tgcggccatc gaagtagcgc gggtcgaaat
     4261 cgtagttgcg gatgagcgtg ggcgcaccct tggtccaggc cacttgcgag cagccggcca
     4321 tgtacggcgg cgggcaccac atgctgagga agcggcaggc cgcgtcgtcg ttgttcgggc
     4381 cgtcgtcgca ggcctcgacg ccggcgtgga cgaagccgtc gccgcaggtg gcgagcttgc
     4441 agtcgccgac gcagctgtcg gtgtcgtcca tgtcgccgtc gtcgcactgc tcgagcgccg
     4501 gctggacgac gccgtcgccg cagaacgccg cggtgcagtc gttgctgcag gcgtcggtgt
     4561 cgtcggtgtt gccgtcgtcg caggcctcgt cgccctc
//
LOCUS       DAOQOC010000077         4593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8121_length_4593_cov_2.673645, whole genome shotgun sequence.
ACCESSION   DAOQOC010000077 DAOQOC010000000
VERSION     DAOQOC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8121_length_4593_cov_2.673645"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1046)
                     /locus_tag="PL106_02040"
     CDS             complement(99..1046)
                     /locus_tag="PL106_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31878.1"
                     /translation="MDSTLSFERLFGMVARHWRLFSGVALLAAALSALFSGPSFITPR
                     FRSSAIVYPVNLASYSIEPTSDQLLQLLESNSIRDSLMRIHHLAEHYDVDTTVAPGRF
                     YLEAEYRDHVSISKTSLESVQIEIEDEDPATAQAMVQDLLDQANKLARRLRREKSEEV
                     LRIAQREMDLARHKLDSVEARLDTLRARTGLLNYNAQTNEVTRGYMRLLATGTPAARA
                     EARQLLDALSAHGGEFNTLSELAYSYRLHFIEKQTAYERVLTDLNKVLTYTDVLVYPE
                     RADKKVFPVRWLIVLVSVCSAGLLCLVFVMLREQRKPAA"
     gene            complement(1039..2058)
                     /locus_tag="PL106_02045"
     CDS             complement(1039..2058)
                     /locus_tag="PL106_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNA31879.1"
                     /translation="MQNEQRGGNGDAIDLMVFLWRQRRLLIGITLLGAVIGVVVAFVI
                     PEKFRGEVILYPAISNSASRSLLNEQSGSRDDILALGDEEDAQQLLQMLQSDEIRERT
                     ATEFDLMNVYRIDADGGHKRAELREAYEDHVTFEFTKFGSVRVEVLDEDPLRAANMAN
                     FIASEVDSVWNAMQMERAAIGVRLVESKVAEAERNVAFLNDSLGRIRAAGVQDYHTQS
                     ERFNEYLGAAIVKGDDRAIRLFDEKFKVLAQYGGAYLTLQGQLDNELWRLNQLRTKLT
                     QARADMESDLPHKFVVNKALPADQKAYPVRWLVCAVSTASALLLALLFLVVRENMDRI
                     RQAHG"
     gene            complement(2130..3593)
                     /locus_tag="PL106_02050"
     CDS             complement(2130..3593)
                     /locus_tag="PL106_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNA31880.1"
                     /translation="MRRVFVSNLALVLVLNLLVKPFYILGIDAAVQVRVGTEAYGGYA
                     ALLSLSFLLNILLDLGITNWNTRHVARDVASMRGQFNGVVVLRVLLVLLYAGVLFLAA
                     FVLGYRGAEVQLLAVLVLNQALAATILFLRGNIAAAQRFAQDSLLSVLDRALLIGLCA
                     WLLWGRGTGAPFNILWFAWAQTLAYGITAMVALVLVVRHTGPPRPRWDPAFVKGVLRQ
                     SAPYALLVLLMSFYYRTDTVMLERLLPDGKLHAGIYAQGFRFFEAFNMLGYLLAGLLL
                     PMFSRLLGTGASVAPLAGLATRLVLAGAIAVAVIGSFHAQEVMDLRYDEHTDASAPAF
                     AVLIWSFVAVCITYVFGTLLTASGDLRALNFMAAGGMVLNIALNLVLIPRYQALGAAW
                     AGLITQGATALVQVAIAARRFDLRPQWGPAFGLVGYATALLLLAWLSEAREMPLLLAV
                     PVLGAAAVGLAAVAGLLPLSGLKGLIVQWSDKRKAGP"
     gene            3689..>4593
                     /locus_tag="PL106_02055"
     CDS             3689..>4593
                     /locus_tag="PL106_02055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA31881.1"
                     /translation="MRIAVNTRLLLPGKLEGIGWFAHETLSRIVVAHPEHKFIFLFDR
                     PYDRSFVYADNVEAVVVPPPTRHPLLYRLWFQFALPRALRRNKADVFLSPDGFLSTRT
                     AVPQLAVMHDLNFEHYPEDLPAAYSTYYRTWFPRFAKQAARIVTVSEFSKSDIASKYR
                     IDPAGIDVVHNGVSGLFSPISMAEALQARARFTGGSPYLICVGAMHRRKNLANTLAAF
                     DRVAEDEAGLRLLIVGERMFRDARLEQAYAAMRHKDRVFFAGRLGQQDLRLALGAALA
                     LVYASWFEGFGIPVAQWLRGPLRQHV"
BASE COUNT          821 a         1547 c         1478 g          747 t
ORIGIN      
        1 ccagtactgc tcaaaggcca tcagcaccgc gttcagcagg atgaaggtcg tgcagacgac
       61 cagtatccaa cggtccttga gcaggttgaa cgccatgatc aggcagcggg cttccgctgt
      121 tcgcgcagca tgacgaagac caggcagagc agaccggcgg agcacacgga caccagcacg
      181 atcaaccagc gcacggggaa caccttcttg tcggcccgtt ccgggtatac cagcacatcg
      241 gtataggtga gcaccttgtt caggtcggtg agcacgcgtt cgtaggctgt ttgtttctcg
      301 atgaaatgga ggcggtagct gtacgcgagt tcgctgagcg tgttgaattc accaccgtgc
      361 gcgctcaacg catcgagcag ttgacgggcc tcggcacgtg cggccggggt gccggtggcc
      421 aggaggcgca tgtacccgcg cgtcacctcg ttggtctggg cgttgtagtt cagcaggccg
      481 gtgcgcgcgc gcagggtgtc caggcgtgcc tccacactgt ccagcttgtg acgggccagg
      541 tccatctccc gctgcgcgat ccgcagcacc tcctcgctct tctcacgccg caggcgacgg
      601 gccagcttgt tggcctggtc cagcaggtcc tgcaccatgg cctgcgccgt ggcggggtcc
      661 tcgtcctcga tctcgatctg cacactctcc aaactggtct tgctgatgga cacgtgatcg
      721 cggtactcgg cctccaggta gaagcggccg ggcgccacgg tggtgtccac gtcgtagtgc
      781 tcggccagat ggtgaatgcg catcaggctg tcgcggatgc tgttgctctc cagcagttgc
      841 agcaactgat cgctggtggg ttcgatggag tagctggcca ggttcacggg gtacacgatg
      901 gccgaggagc ggaagcgcgg ggtgatgaag gagggaccac tgaacagcgc actgagtgct
      961 gcggccagca gggccacgcc ggagaagagc cgccagtggc gggccaccat cccgaacagg
     1021 cgttcaaagg agagcgtgct atccatgggc ctggcgaatg cggtccatat tctcccgaac
     1081 caccaggaag agcaacgcca gcaacagggc gctggcggtg ctcacggcgc aaacgagcca
     1141 gcgcacgggg taggccttct ggtccgcagg cagcgctttg ttcactacga acttgtgcgg
     1201 caggtcgctc tccatatcag cgcgcgcctg ggtgagtttg gtgcgcagtt ggttgagacg
     1261 ccagagctca ttgtccagtt gtccctgcaa ggtgaggtag gcgccgccgt actgggcgag
     1321 cactttgaat ttctcatcga acaggcggat cgcgcgatcg tcgcccttca cgatggccgc
     1381 accgaggtac tcgttgaagc gctcgctctg ggtgtggtag tcctgcacgc ccgccgcgcg
     1441 gatgcggccg aggctgtcgt tcaggaaggc cacgttccgt tcggcctcgg ccaccttgct
     1501 ctccaccagc cgcacaccga tggcggcgcg ttccatctgc atggcgttcc acacgctgtc
     1561 cacctcgctg gcgatgaagt tggccatgtt cgccgcgcgc aggggatcct cgtccagcac
     1621 ctccacgcgt acgctaccga atttggtgaa ttcaaaggtg acgtggtcct cgtaggcctc
     1681 gcggagttcc gcgcgcttgt ggccgccgtc cgcatcgatc cggtacacgt tcatcaggtc
     1741 gaattccgtg gcggtacgct cgcggatctc gtcgctctgg agcatctgca acagctgctg
     1801 cgcgtcctct tcatcgccca aggcgaggat gtcgtcccgc gaaccgctct gctcgttcag
     1861 caaggagcgg ctggcgctgt tgctgatggc ggggtacagg atcacctcgc cccggaactt
     1921 ctcggggatc acaaaggcca ccaccacccc gatcacggca cccagcaggg tgatcccgat
     1981 caggagcctg cgttggcgcc agaggaagac catcaggtcg atggcgtccc cgttgccgcc
     2041 gcgctgttca ttctgcatgc gtctggtata aaggatgaac ctgcgctcga ggcttcggtt
     2101 cagaaggcgg cgaagatacc cgcccctcgt caagggcccg ccttgcgctt atcggaccac
     2161 tgcacgatca gccccttcag gccgctcaac ggcagcagac ccgccacggc cgccaggccc
     2221 accgccgccg cgcccagcac cggtacggcc agcagcaggg gcatttcccg ggcttcggag
     2281 agccaggcca gcagcagtag cgcggtcgcg taccccacca gtccgaacgc agggccccac
     2341 tgcggtcgca gatcgaaccg gcgcgctgcg atggccacct gcaccagcgc cgtggcgccc
     2401 tgagtaatga gcccggccca cgcggcaccc agggcctggt agcgcggaat gagcaccaaa
     2461 ttgagcgcga tgttgagcac catgcccccg gcggccatga aattcagcgc gcgcagatcg
     2521 ccgctggccg tgagcagcgt accgaagacg taggtgatgc acaccgccac gaaactccag
     2581 atgagcaccg cgaaggccgg agccgaggcg tcggtgtgct catcgtagcg caggtccatc
     2641 acctcctggg catgaaagga cccgatcacc gccacggcga tggccccggc cagcaccaag
     2701 cgcgtggcca ggccggccag gggcgcaacg ctggcgccgg tccccagcag gcgactgaac
     2761 atggggagca gcaggccggc cagcaggtag cccagcatgt tgaacgcctc gaagaagcgg
     2821 aagccctgcg cgtagatgcc cgcgtgcagt ttgccgtccg gcagcaagcg ctccagcatc
     2881 accgtatcgg tgcggtagta gaagctcatc agcagcacca gcagggcgta gggtgcgctt
     2941 tggcgcagca cacctttgac gaaggccgga tcccaacgcg ggcgcggcgg cccggtatgg
     3001 cgcaccacca gcaccagagc caccatcgcg gtgatgccgt aggccagggt ctgcgcccaa
     3061 gcgaaccaga ggatgttgaa cggggcaccg gttcctcgcc cccagagcag ccaggcgcac
     3121 aggccgatca gcagcgcccg gtccagcacg ctgagcaggc tgtcctgcgc gaagcgctgc
     3181 gccgccgcga tattgccccg caggaagagg atcgtggccg caagagcctg gttcagcacc
     3241 agcacggcca gcagttgcac ctcggcgccc cggtagccca gtacgaacgc ggcaaggaag
     3301 aggacccccg cgtacagcag caccagcagg acccgcagca cgaccacccc gttgaactgg
     3361 ccgcgcatgc tggcgacatc gcgcgccacg tgccgcgtgt tccaattggt gatgcccagg
     3421 tccagcagga tgttcagcag gaagctcagg ctcagcaacg ccgcatagcc tccataggct
     3481 tccgtgccca cgcgcacctg caccgccgca tcgatgccca ggatgtagaa gggcttcacc
     3541 agcaggttca gcaccagcac cagggccagg ttggagacga agacgcggcg catgcgggat
     3601 acgcgggcga atgtgggaaa tgcggttcac gcgccaacga ggccgatccc agcaatgcgc
     3661 acattcgtgc ccggttctgc tccttgtgat gcgcatcgct gtgaacaccc gcctgctatt
     3721 gcccggcaag ctcgaaggca tcggctggtt cgcgcacgaa acgctgtcgc gcatcgtggt
     3781 ggcgcatccc gagcacaagt tcatcttcct cttcgaccgg ccgtacgacc ggagcttcgt
     3841 gtacgccgac aacgtggaag cggtggtcgt gcccccgccc acgcgccacc cgctcctata
     3901 ccggctctgg ttccagttcg cgctcccgcg tgccctgcgc cgcaacaagg ccgacgtctt
     3961 tctctcgccc gatggcttcc tcagcacccg cacggccgtc ccccagctcg cggtgatgca
     4021 cgacctcaac ttcgagcact accccgagga cctgcccgcg gcctacagca cctactaccg
     4081 cacctggttc ccgcgcttcg cgaagcaggc cgcccgcatc gtcacggtga gcgaattcag
     4141 caagagcgac atcgcatcca agtatcgcat cgatcccgcc ggcatcgacg tggtacacaa
     4201 cggggtgagc ggactgttct cgcccatcag catggccgaa gcgttgcagg cccgggcgcg
     4261 cttcaccggg ggctcgccct acctgatctg cgtgggcgcc atgcaccggc ggaagaacct
     4321 ggccaacacg ctggcggcct tcgaccgcgt ggccgaggac gaggccgggc tgcggctgct
     4381 gatcgtaggg gagcgcatgt tccgcgatgc gcggctggag caggcctacg ccgccatgcg
     4441 ccacaaggac cgcgtattct tcgccggtcg gttgggccag caggacctgc gactcgccct
     4501 gggcgccgcg ctcgccctgg tgtacgccag ctggttcgag ggcttcggca tccccgtggc
     4561 ccagtggcta cggggcccgc tgaggcagca cgt
//
LOCUS       DAOQOC010000078         4592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8123_length_4592_cov_4.096760, whole genome shotgun sequence.
ACCESSION   DAOQOC010000078 DAOQOC010000000
VERSION     DAOQOC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8123_length_4592_cov_4.096760"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="PL106_02060"
     CDS             <1..1753
                     /locus_tag="PL106_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNA31882.1"
                     /translation="VLWCDRQPDRGPDHLDLKLPREGGSLLLIDPDRRTVRDFYSWRS
                     LPAGVSLGRLTDGGAEWGFFTAPTPGGSNQRSQGATRWLPAPEPVITDGVLTCAAPEN
                     GTIRWTTDGRTPDTRSPELVGTLTIAPPCVITLRAFGEDALPSAPVSFTFPSEGHGPF
                     VAIVVDPDSLNDPRRGILAPEHANYARTGIDWRRDALVEWHTNDSVCHEAVRIAVHGS
                     GTRGLAKKSFKLFGDGEMILRADASPHAFLRHLFIESVARDAEVDVQPGSPMPLWLNG
                     AYHGLYRAMPAKNSDWLRSLSGAEAIDLIDGPGARALKGDDDGHQRLLTMIERGAPLE
                     ELDGLMEETSLLDLACFDLWTGRADHDLNTRCWRPRERGGRWRWILFDVDLWSPPDEG
                     TVERMCSAIAPESPYLPWLLRHEELGPHLLARLSAWMATSLAPDRAIRLADSLFAANA
                     EPMRADHIRWRDATGIPSPKESIAALRAHISARPEHLIGQLERYTRHRAREVTVRVSP
                     PDAGEVLISTLPLTRDERTFTAFAGAPVQLTAIPAPGRVFAGWKGIAATSPMIQVDPA
                     KVKGIHALFRLATEEMP"
     gene            complement(1760..2449)
                     /gene="purQ"
                     /locus_tag="PL106_02065"
     CDS             complement(1760..2449)
                     /gene="purQ"
                     /locus_tag="PL106_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNA31883.1"
                     /translation="MKFGVVVFPGSNCDEDMVHVLEHGMGQRVERLWHKEHDLKGCDM
                     IVLPGGFSYGDYLRSGAIARFSPIMREVSAFAQKGGLVLGVCNGFQVLCEAGLLPGAL
                     LHNEGQQFICRNVFIRAQTRNTPLTSAVNDGPLNIPIAHGEGRYYADAATIKALNDND
                     QVLFRYCDAEGRLRDDANPNGSVENIAGVCNAGRNVFGMMPHPERAADERLGNTDGKA
                     LFRSLLQGVPA"
     gene            2637..3713
                     /locus_tag="PL106_02070"
     CDS             2637..3713
                     /locus_tag="PL106_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNA31884.1"
                     /translation="MTIPPVITDPVYDPATRHSALDRFFLRLIRDPRDLPFVYLGLRV
                     SLLLIPMAVLLYMPFVTGWVWWAIALVYLWINNITLKGPFGLMLHCTTHRAFFKKEYG
                     LLNHYYPWVLAPLFGQSPETYRAHHVGMHHVENNLEPDESSTMPYRRDSGRDFLRYYF
                     RFLFTGIFTLSFYLFGHNRKKLAWQAIRGEILFVVFCTVMCFVNAPATIVAFIIPFLA
                     FRLVAMAGNWTQHTFIDPQDPGNHFTNSITCINTKFNHRCWNDGYHISHHIDPTMHWT
                     EHPKFFLENLAEFGRQKAVVFTGLNYVDVFTLLLRDRYDTLADHFVDAGGHFTGRKDE
                     LIAFLRSRTVPIPFTHDEELPTAA"
     gene            complement(3747..>4592)
                     /locus_tag="PL106_02075"
     CDS             complement(3747..>4592)
                     /locus_tag="PL106_02075"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNA31885.1"
                     /translation="GLDFDSLLIDRCMFTNNTSELSILEFDGFNAARLSNTYIIGNTV
                     NGSTSNVFRVQTTAPDEAMTINNCTIAQNFNLASSVQQEIIRVYGTQHRIENSILAGN
                     TAYNGRQVSTGMVITNSIVPGGHPNGTGILDMDPLFAQPSFASGTNFDATSFDYTLLP
                     ASPGINAGTNDLVPVDQLLDLAGNVRIQGGTVDMGCYESDLSTGVSPNPAPATTWYFD
                     SQQQALRYTGTQDMSGRVLRIIDANGGQVATIPVALNTVQVSLSPGMYLAITGKADAF
                     RFVVP"
BASE COUNT          816 a         1497 c         1389 g          890 t
ORIGIN      
        1 ggtgctctgg tgcgaccgcc agccggacag ggggcccgat catctggatc tcaaactgcc
       61 ccgggaggga gggagtttgt tgctgatcga tcccgatcgc cgcactgtgc gggatttcta
      121 cagttggcgt tcgttgcctg cgggggtctc ccttggccgt ttgactgacg gtggtgcgga
      181 gtggggcttc ttcactgcac caaccccggg cggatccaac cagcgctccc agggagcaac
      241 tcgctggctg ccggcgccgg aacccgtcat cacggatgga gtactcacct gcgctgcacc
      301 agaaaacgga acgatccgat ggaccacgga cgggcgcact cctgacaccc gctcacccga
      361 gcttgtcggc accctgacga ttgcaccgcc ctgcgtgatc accctgcgtg ccttcgggga
      421 ggatgcgtta ccgtccgcac cggtatcatt caccttcccc tcggaaggcc atggaccatt
      481 cgtggccatt gtcgtcgacc ccgacagctt gaatgatcct cggcgtggta tcctggctcc
      541 cgaacatgcc aactatgccc gaacaggtat cgattggcgg cgcgatgccc tggtcgagtg
      601 gcataccaac gacagcgtgt gccatgaggc ggtgcgcatc gccgtgcatg gcagcggtac
      661 gcgcgggctg gccaagaaga gtttcaagct gttcggcgac ggggaaatga tcctgcgtgc
      721 ggacgcctcc ccgcatgcgt tcctccggca cctcttcatc gaatcggtgg cccgtgatgc
      781 cgaagtggac gtgcagcccg gttcgccaat gcccctctgg ctcaatggcg cgtaccacgg
      841 gctctaccgc gccatgccgg ccaagaacag cgactggctg cgcagcctga gcggagcgga
      901 ggccattgac ctcatcgacg ggccgggcgc gcgtgcgttg aagggggatg acgatggcca
      961 ccaacgtctg ctgaccatga tcgaacgcgg tgctccgctg gaggaactgg atggactgat
     1021 ggaggagacg agcctgcttg acctggcctg cttcgatctc tggaccggac gtgccgacca
     1081 cgacctgaac acgcggtgct ggcggccgcg cgaacggggc gggcgctggc gctggatcct
     1141 gtttgacgtg gatctttggt cgccgccgga cgagggcacc gtggagcgca tgtgttcggc
     1201 catcgccccg gaatcgccct acctgccctg gttgctgcgc catgaggaac tgggaccgca
     1261 cctgttggcg cgcctctcgg catggatggc cacgtcattg gcgccggacc gcgccatccg
     1321 cctggccgat tcgctcttcg cggcgaacgc ggagcccatg cgggccgatc acatacgctg
     1381 gcgggatgca acgggaatac catccccgaa ggagagtatc gcagcacttc gtgcgcacat
     1441 cagcgcacgc ccggagcacc tcatcggcca gttggagcga tacacccggc accgcgcccg
     1501 ggaggtcacg gtgcgcgttt caccaccgga cgccggtgag gtgctgatca gcaccctgcc
     1561 gctcacccgc gatgaacgca cgttcaccgc attcgccggg gcacccgtgc aactcacggc
     1621 catccccgca ccgggcaggg tgttcgcggg gtggaaaggc attgctgcca caagcccgat
     1681 gatccaggtt gatcccgcca aggtgaaggg gatccacgcg ttgttcaggc tcgcgaccga
     1741 ggagatgcca taaccctggt tacgccggga ccccttgcag cagcgaccgg aataatgcct
     1801 taccgtcggt attgcccagg cgctcatcgg cggcccgctc cggatggggc atcatgccga
     1861 acacgttgcg gcccgcattg caaacacccg cgatgttctc cacgctgccg ttggggttgg
     1921 catcgtcgcg caggcggcct tcggcatcgc aatagcggaa cagcacctgg tcattgtcgt
     1981 tcaacgcctt gatggtggca gcgtcagcgt aataacggcc ttcgccatgc gcgatcggga
     2041 tgttcagcgg cccatcgttc accgcgctgg taagtggcgt attccgcgtc tgtgcccgga
     2101 tgaagacgtt gcggcagatg aactgctggc cctcgttgtg caataacgca ccgggcagca
     2161 atccggcttc gcagagcacc tggaagccgt tgcacacccc gagcaccaga cctcctttct
     2221 gcgcgaaggc acttacttcc cgcatgatgg gggagaaccg cgcaatggca ccgctccgca
     2281 ggtaatcgcc gtagctgaag cccccgggca gcacgatcat gtcgcagccc ttcaggtcgt
     2341 gctccttgtg ccagaggcgt tccacacgct ggcccatgcc gtgctccagc acgtgcacca
     2401 tgtcctcatc acaattgctg ccggggaaga cgacgacgcc gaatttcatg cgaagcggtg
     2461 ttgggaccgc gaaaataggg cgacctggtc gcatgcatcc caccggccat ggtgtttcct
     2521 tggcccacaa cgttcgtggg ttttcctgtc ccgcttgcgg aaatggatgt tgaccaggta
     2581 acggagccga gtggatgtca ggcccgacct catcgcgaaa cccgatattg cgcctcatga
     2641 ccatcccacc tgtcatcacc gatccggtgt acgatccggc cacccggcac agcgccttgg
     2701 accgcttctt cctgcgcctc atccgcgatc cgcgcgatct gcccttcgtg tacctcgggc
     2761 tgcgcgtctc gctcctgctg atccccatgg ccgtgctgct gtacatgccc ttcgttacgg
     2821 gctgggtctg gtgggccatc gcgctggtgt acctctggat caacaacatc acgctcaaag
     2881 gccccttcgg gctcatgctc cactgcacca cgcaccgggc gttcttcaag aaggaatacg
     2941 gcctgttgaa tcactactat ccctgggtac tggccccgct gttcggacag agccccgaga
     3001 cctaccgcgc gcaccacgtg ggcatgcacc atgtggagaa caacctggag ccggacgaga
     3061 gcagtaccat gccctaccga cgcgattcgg ggcgcgattt cctccggtat tacttccgct
     3121 tcctgttcac cggtatcttc acactgtcgt tctacctgtt cgggcacaac aggaagaaac
     3181 tggcttggca ggccatccgg ggcgagatcc tcttcgtggt cttctgcaca gtgatgtgct
     3241 tcgtgaacgc acccgccacc atcgtggcct tcatcatccc cttcctcgcc ttccgcttgg
     3301 tggccatggc cggcaactgg acccagcaca ccttcatcga cccgcaggat ccgggcaatc
     3361 atttcaccaa cagcatcacc tgcatcaata cgaagttcaa tcaccgctgt tggaatgacg
     3421 gctaccacat cagccaccac atcgatccca ccatgcactg gaccgagcac ccgaagttct
     3481 tcctggagaa cctggccgag ttcggcaggc agaaggcggt cgtcttcacg ggcctgaatt
     3541 acgtcgacgt gttcaccctg ctgcttcgtg acaggtacga tacgctggcc gaccacttcg
     3601 tggacgccgg tgggcacttc accggcagga aagatgaact gatcgccttc ctgcgcagcc
     3661 gcaccgtccc catccccttc acgcacgacg aagagctgcc cacggcggcc tgatgcccgt
     3721 tccgccgggc ctcgccttcg ctccgctcag ggaaccacga accggaacgc gtccgccttg
     3781 ccggtgatgg ccagatacat ccctggcgat aatgatactt ggaccgtgtt cagggctacg
     3841 ggaatggtgg ccacctgtcc accgttcgca tcgatgatcc gcagaacccg ccctgacatg
     3901 tcctgtgtgc ccgtgtaccg cagcgcctgt tgttgggaat caaagtacca ggtggtcgca
     3961 ggtgccgggt tcggggaaac accggtgctg aggtccgatt cgtaacatcc catgtccacg
     4021 gtaccccctt ggatgcgcac gttccccgcc aggtccagaa gctggtccac tgggaccagg
     4081 tcattcgtgc cggcgttgat gccgggcgag gccggaagca gggtgtaatc gaacgaggtg
     4141 gcatcgaaat tggtgcccga tgcgaacgat ggctgcgcga acaaagggtc catatcgagg
     4201 atccccgtgc cattcgggtg cccacccgga acgatcgaat tcgtgatcac catgccggtg
     4261 ctcacctgtc gcccgttgta ggcggtgttc cctgccagga tgctgttctc gatccggtgt
     4321 tgagtgccgt agacccggat gatctcctgt tgcaccgacg aggccaggtt gaagttctgc
     4381 gcgatcgtgc agttgttgat cgtcatcgct tcgtcggggg ccgtggtctg caccctgaac
     4441 acgttggagg tgctgccgtt caccgtgttg ccgatgatgt aggtgttgct gagccgggcc
     4501 gcgttgaacc catcgaactc caggatgctc agttccgagg tgttgttggt gaacatgcac
     4561 cgatcgatga gcagcgaatc gaagtcgagc cc
//
LOCUS       DAOQOC010000079         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8143_length_4586_cov_3.564114, whole genome shotgun sequence.
ACCESSION   DAOQOC010000079 DAOQOC010000000
VERSION     DAOQOC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_4586_cov_3.564114"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1166)
                     /locus_tag="PL106_02080"
     CDS             complement(165..1166)
                     /locus_tag="PL106_02080"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNA31886.1"
                     /translation="MFRLSTFAFAVLITALAKGQTSNLIIFSEMGEKFTLIVDGEVKN
                     EVPATRVVATGLKSATPSILVKFADAGIPPVKQNAWLEPDMEYTLKLTTNKKGEHVLR
                     MQGQAALGTSTSTSTFVEDPPATISDPAIADPPAPSAPQGTGGTGSVTTTTTQTTGTT
                     GAVNMNVGINGVGVNMSVGITDPLAATTTTTTTTTMTTTTTTTTGQNDDTDQETASTP
                     VVEEVVQRMPGYNGPIGCMSLPMGETDFNEAKATIESKGFEETKLTVAKQIGDNNCFS
                     VAQVKAVMGLFGFEETRLDFAKYAYDHTHDIGNYYKVNDAFSFSSSIDELSTYLKSR"
     gene            1385..2344
                     /locus_tag="PL106_02085"
     CDS             1385..2344
                     /locus_tag="PL106_02085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA31887.1"
                     /translation="MDLSVVIPLYNERESLPELAGRLHEVIGKMGLRYEVLFVDDGSN
                     DGSWEVVERLHREDPRVKGIKLGRNYGKSPALNEGFSAAQGLVVITMDADLQDDPAEV
                     PELYRMIAQDGFDLVSGWKKKRYDPLSKTIPTKLFNWTTRRASGVMLHDFNCGLKSYR
                     RDVVKSIEVFGEMHRYIPFIAKKEGFHRIGEKVVEHHPRKFGATKFGLDRFINGFLDL
                     LTITFVFRFGKKPMHFFGAMGTLMFMLGSLATAWIIGEKLYLNFVRGIQVTRPVTDQP
                     LFFLALVAMIIGVQLFVAGFVAELVARNSPVRNAYRVDRRLGI"
     gene            2354..3778
                     /locus_tag="PL106_02090"
     CDS             2354..3778
                     /locus_tag="PL106_02090"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HNA31888.1"
                     /translation="MDASHSTRWPVILPLTLLLGLHAYLGRYAHPMADDFSYALKDIS
                     LGAWKAALWEYQHWNGRYASNFLVLFGPLRSGFDGLPLYRMIPGLLLALTLAGAYTFI
                     RTIGRTLPRTDALMLALVQIALFTHLMPDLPEGFYWYTGSVSYQLPNALSLFAAALIV
                     RGSRDGKRWAYLAAAPLLFLITGCCEVSMVLAVIAVLLLYSATAMRGQGPSNGLPWLL
                     LAVLLGALLMILAPGNEGRGALFPVRHQFFHSLGMSALQSVRFSITWAACPALLLASA
                     LWLLHRQRLAAHIPGLADGFGLRPWMTIAALAGVTFLCAFPAYWSTGILGQHRTINTA
                     CFVFIPLWFLNLSVLAARVPRARLSVHPRDLPRTTIAFVVLALIDLGLSGNGGKAMRD
                     MFSGRAQRSDAQLWQRYDLLRAAAATPEGIAEIPLIKDPPSSIYVLDLRGPDFLVNRD
                     YAAWFGLKEVRISGSASTDGKRPN"
     gene            complement(3824..>4586)
                     /locus_tag="PL106_02095"
     CDS             complement(3824..>4586)
                     /locus_tag="PL106_02095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA31889.1"
                     /translation="SIVDNAIPHEPRFFDKPFTRYFLEHNDGFVAMSERVKEDLLALR
                     PDARVALLPHPLYDHFGPPVPMADARRRLGIAEDRKVSLFFGFIRDYKGLDLLIDAMA
                     ELPPDHVLVIAGEPYGDMRKYHEAIARNGLKDRVVDHIRYIADAEVPVFFSAADAVVL
                     PYRSATQSGITAIAYHFGVPVIATDVGGLKEAVEDGRTGIIVPHPDAVDIAQAIRRFH
                     AEHLATACRARVAHLREELSWGRFADRLGTFAASL"
BASE COUNT          786 a         1443 c         1409 g          948 t
ORIGIN      
        1 tacatatcgg ccgcctcggc gtacagcccg cccgcttcga gcttcatgcc cttcttggcc
       61 atggacttcg agccggagca cgccgccaag gtgagcgcaa caagggccag tatgaatgtg
      121 atcggcttca tggcatggcg tcctgtacgt ggacggtccg ttgatcaacg gctcttcaga
      181 taggtgctca actcatcgat ggagcttgag aagctgaacg catcgttcac cttgtagtag
      241 ttgccgatgt cgtgggtatg gtcgtacgcg tacttggcga agtccaaacg cgtttcctcg
      301 aaaccgaaga ggcccatgac cgccttcacc tgggccacac tgaagcagtt gttatcgccg
      361 atctgcttgg ccacggtcaa cttggtctcc tcgaagcctt tgctttcgat ggtcgctttg
      421 gcctcgttga aatcggtttc acccatgggc agcgacatgc aaccgatggg gccgttgtac
      481 ccgggcatgc gctgaaccac ctcctcaacg acgggagtgc tggccgtttc ctggtcggta
      541 tcgtcgttct gcccggtcgt ggtcgttgtc gtggtggtca tggtcgtggt ggtcgtggtg
      601 gtggtcgtgg ccgccaaggg atcggtgatg cccacggaca tgttgacgcc cacaccgttg
      661 atccccacat tcatgttgac cgcgccggtg gtacccgtgg tctgtgtggt cgtggtggtc
      721 acggaccctg tacctccggt accctggggt gcgctcgggg caggtggatc cgcgatggcc
      781 ggatcggaga tggtggccgg gggatcctcc acgaaggtgg acgtgctggt ggatgtaccc
      841 aatgcggcct gtccctgcat ccgcaacacg tgttcccctt tcttgttcgt ggtgagcttc
      901 aaggtgtact ccatgtccgg ttcgagccag gcgttctgct tcaccggagg aatgcccgca
      961 tcggcgaact tcaccaggat ggagggcgtc gccgacttca gcccggtggc caccacccgc
     1021 gtggcaggta cctcattctt cacctcccca tccacgatca gtgtgaattt ctcacccatc
     1081 tcggaaaaaa tgatgaggtt gctggtctgg cccttggcga gagccgtaat gagaacagcg
     1141 aaagcgaagg tggagagacg gaacatgatc ggtggttttt gtggggtcgg cgaatgtagt
     1201 ccgcttcaaa atggcaagag acgtgccact gcggtacgga gggacctttt tccacgatcc
     1261 accgggtatc cgatgagtat gtatgtgtcc tcggttctct tgccgataat aaccgggtgt
     1321 gtccggtgcc cgccaaaggc ttggacaggc tttctttgca cgccttcgtc ccggcaaacc
     1381 ccgcatggac ctgtccgtcg tcatcccctt gtacaacgag cgcgaatcgt tgccggaact
     1441 ggccggacgc ttgcacgagg tgatcggcaa aatgggcctg cgctacgagg tcctcttcgt
     1501 ggacgatggc agcaacgacg gctcgtggga ggtggtggag cgcttgcacc gcgaggatcc
     1561 gcgggtgaag gggatcaagc tgggtcgcaa ctacggcaag agccccgcct tgaacgaagg
     1621 cttcagcgcc gcacagggcc tggtggtgat caccatggat gcggacctgc aggacgaccc
     1681 ggccgaggta ccggagttgt accgcatgat cgcgcaggac gggttcgacc tggtgagcgg
     1741 ctggaagaag aagcgctacg accccctctc caagaccata cccaccaaac tcttcaactg
     1801 gaccacccga cgcgcgagcg gcgtgatgct gcacgatttc aattgcggac tgaaatcgta
     1861 ccgccgcgac gtggtgaaat ccatcgaggt cttcggggag atgcaccgct acatcccctt
     1921 catcgccaaa aaggaaggtt tccatcggat cggcgagaag gtggtggagc accatccccg
     1981 caaattcggg gcgaccaaat tcggcctgga ccggttcatc aacggcttcc tggacctgct
     2041 caccatcacc ttcgtgttcc gcttcgggaa gaaacccatg cacttcttcg gcgccatggg
     2101 caccctgatg ttcatgctcg ggtcgctggc cacggcatgg atcatcggcg agaaactgta
     2161 cctgaatttc gtgcgcggga tacaggtgac acgacccgtg accgaccagc cgctgttctt
     2221 cctggcactg gtcgccatga tcatcggcgt gcaactcttc gtggctggtt tcgtggccga
     2281 actcgtggcc cgcaactccc cggtgcgcaa cgcctaccgc gtggatcgcc ggctcggtat
     2341 ctgaccgcgc gacatggacg cgtcgcattc cacgaggtgg ccggtcatcc tgccgctcac
     2401 gctgctgctg ggcctgcacg cctacctcgg ccggtacgcg caccccatgg ccgatgactt
     2461 ctcctatgcg ctgaaagata tttccctggg tgcgtggaag gccgccctgt gggagtacca
     2521 gcactggaac ggccgctacg cgagcaactt cctcgtgctt ttcggtcccc tgcgttcggg
     2581 tttcgatggt cttccacttt accggatgat ccctgggctc ctgctggcgc tgacactggc
     2641 cggcgcctac acgttcatcc gaacgatcgg gcgcacgttg ccccgcaccg atgccctgat
     2701 gctcgcgctc gtccagatcg cgctcttcac ccacctgatg cccgacttgc cggagggctt
     2761 ctactggtac acgggttccg tttcgtacca gttgcccaat gcgctgagcc tgttcgctgc
     2821 ggcgctgatc gtacgcggat cgcgcgatgg gaagcggtgg gcgtacctcg cggccgctcc
     2881 gctcctgttc cttatcacag ggtgctgcga ggtgagcatg gtactggcgg tgatcgccgt
     2941 gctgctgttg tacagcgcga ccgcgatgcg cggccaagga ccgtccaacg gtctgccatg
     3001 gctgctgctg gcagtgctcc tgggcgctct tctcatgatc ctggcgcccg gtaatgaagg
     3061 gcgaggcgcg ttgttcccgg tccggcatca attcttccat tcgctgggta tgagcgcact
     3121 gcagtccgtg cgcttcagca tcacgtgggc cgcctgcccc gcgctcttgc tggccagtgc
     3181 gttgtggctc ctccatcgcc agcggctggc ggcacacatc ccggggctgg ccgatggctt
     3241 cggtctgcga ccttggatga ccatcgccgc gctcgcgggc gtcaccttcc tgtgcgcctt
     3301 cccggcgtac tggagtacgg ggatcctcgg ccagcaccgc accatcaata cggcgtgctt
     3361 cgtgttcatc ccgctctggt tcctgaacct cagcgtgctc gccgcccggg tccccagggc
     3421 ccgtttgagc gtacaccccc gcgacctgcc ccgcaccacc atcgctttcg ttgtgctcgc
     3481 cctcattgat ctcggtcttt ccggcaacgg tggcaaggcc atgcgggata tgttctccgg
     3541 gcgtgcccaa cgctcagatg cccaattatg gcagcggtac gacctgctcc gtgcggccgc
     3601 cgcaacgccc gagggcatcg ccgagatccc cctgatcaag gatccgccca gcagcatata
     3661 cgtgctggac ctgcgcgggc ccgacttcct ggtgaaccgg gactacgcgg cctggttcgg
     3721 attgaaggaa gtacgcatca gcggatcggc aagtaccgat ggcaagcggc cgaactgagc
     3781 ccatgtgcgg atgcgatgac gccgggtcgc catcaccggt tgttcagagc gatgcggcga
     3841 aggttcccag ccgatcggcg aagcgacccc acgagagctc ctcgcgcaag tgtgcgacgc
     3901 gggcacgaca ggccgttgcg agatgttccg cgtggaagcg cctgatggcc tgcgcaatgt
     3961 cgaccgcatc ggggtgcgga acgatgatgc ctgttcgacc gtcctccacg gcttctttca
     4021 gccccccgac atccgtagcg atcaccggca ccccgaaatg gtacgcgatc gccgtgatcc
     4081 cgctctgtgt ggcactcctg tacggcagca cgaccgcgtc cgcagcgctg aaaaacaccg
     4141 gcacttccgc atccgcgatg tagcggatgt gatccaccac gcgatccttc aacccgttcc
     4201 gggcaatggc ctcgtggtac ttgcgcatgt cgccgtaggg ctctccggcg atcaccagca
     4261 catggtccgg cggcagttcc gccatcgcat cgatcagcag atcaagcccc ttgtaatcgc
     4321 ggatgaatcc gaagaagagc gacactttcc ggtcctcggc gatacccagg cgcctgcgtg
     4381 catcggccat gggtacgggt ggcccgaaat gatcgtacag cggatggggc agcaatgcca
     4441 cccgtgcatc cggacgcagg gcaagcaggt cctctttgac ccgctcactc atggcgacga
     4501 aaccatcgtt gtgttccaag aagtaccgcg taaagggctt gtcgaagaaa cgtggctcgt
     4561 gggggatggc attgtccacg atgctg
//
LOCUS       DAOQOC010000080         4565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8210_length_4565_cov_3.602217, whole genome shotgun sequence.
ACCESSION   DAOQOC010000080 DAOQOC010000000
VERSION     DAOQOC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8210_length_4565_cov_3.602217"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PL106_02100"
     CDS             <1..280
                     /locus_tag="PL106_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31890.1"
                     /translation="SSMGLEMRWNTSGKGSVQVNGDLVDITYGGAVNSALGNEMLTGL
                     KPGLNGTWALIIQRRLSDHLQLDLTYNGRHSLGVPITHVGGVQVRAYF"
     gene            complement(303..2762)
                     /locus_tag="PL106_02105"
     CDS             complement(303..2762)
                     /locus_tag="PL106_02105"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNA31891.1"
                     /translation="MSAWCYSLSRIAEAVGGRLLGDGARMVESVSIDSRGSCSATSLF
                     IALRGPRHDGHDHIAGLALQGLRAFMVEEGRAAAFARSDLIVVPDTLLALQRFAAWHR
                     AHFNLPVVGITGSNGKTVVKEWLWQMLRSEELIVRSPGSWNSQVGVPLSVLEMGPAHT
                     LGLFEAGISTAGEMDRLRPVIRPTIGVLTNIGAAHGDGFTDDHEKCAEKLRLFKDAHA
                     VVLGTDNTVVRSVAQAVLRPEVLRDWSHERDAFVRVLNEVERGEATAITLLHAGKRHE
                     YLVPFTDAASRENALTCITLLLQLGRDPHWINEALPQLSPVDMRMRSVEGAQGSTLID
                     DSYSNDPTSLRIALDHLAHMAHGRRRVVVLSDMLGSGLPADLLYQRVAHDLSAAKVDH
                     VIGVGPSISAHRHHFPPGSHFHVDADALLRNEEPKALMGAVVLVKGARDFRLERVVRR
                     WQQHSHGTELEVDLGAIRHNLNHYRAIVGPDIRLMAMVKAAGYGCGALELARLFDHER
                     IHYLGVAYADEGVELRRHGITVPILVMNPEPIPLETLQRFHLEPEVYDERSLAAVLEH
                     AASSGSSTTIHIKLDTGMHRLGFTAADMPWLLEQLRDKRMPHVASILSHLAASEDPAQ
                     DDFTRGQIAAFSSMATRIGEVLGYRPLWHIANSGAVSRFPEARLDMVRLGIGLHGVGV
                     DERETRQLMPVATLRTVIAQLRTVAPGESVSYGRRFIAQRETRIATLPIGYADGLARR
                     LGNAVGKVWINDKAAPFVGTICMDMCMVDVTDIPVDIDDEVIVFGPEHPVQKYARDLG
                     TIPYEALTSISPRVRRVYVQG"
     gene            complement(2759..3352)
                     /locus_tag="PL106_02110"
     CDS             complement(2759..3352)
                     /locus_tag="PL106_02110"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNA31892.1"
                     /translation="MFLERAASRAQRKGWIEVVCGSMFSGKTEELIRRMRRAEFAKQR
                     VEIFKPGVDTRYDAQDVVSHDRNSIRSTPVPNSSNLLLLAGDIEVVGIDEAQFFDAGL
                     PQVCEQLASQGVRVIAAGLDMDFLGRPFGPMPQLMAMAEYVTKVHAICMRCGELATFS
                     HRTSTSRELVVLGETDQYEPLCRTCFFAARAEMEPGT"
     gene            3486..4214
                     /locus_tag="PL106_02115"
     CDS             3486..4214
                     /locus_tag="PL106_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31893.1"
                     /translation="MSENTPAGRMHGLVENLLRALRELDGGRLDRDGLEVACADAQAL
                     YERLIVLRHKAREGARAVSPAAPEEAAASPGGGIVATSEMPPLRLETRPAEVPPRQTS
                     LIDAIAESETPATPTVPPHPTPKAEPPQRVRGTSQPPRKPERTGPGHTETQGSTLGDK
                     LERAPVADLHKAIALSQKFWFVAELFGGHRDRYEQAVNALNAMGSLDEARTFIANEIT
                     AKATRPPAADAIQAFNDLVERRFK"
     gene            4244..>4565
                     /locus_tag="PL106_02120"
     CDS             4244..>4565
                     /locus_tag="PL106_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31894.1"
                     /translation="MLRSIASLLTMGLLLGASAQSPASVKEKARAYVNVLAGPTMHGR
                     GYVLEGDRIAADWIAAQFDRLGLGKLNDQRFEPFTFAVNTFPDTVRAAIDGRALVPGV
                     DFLVD"
BASE COUNT          817 a         1477 c         1472 g          799 t
ORIGIN      
        1 cagcagcatg ggcctggaga tgcgctggaa tacctcggga aaagggtcgg ttcaggtgaa
       61 cggggatctg gtggacatca cctacggggg agcggtcaat tcggccttgg ggaacgaaat
      121 gctcaccggc ctgaagcccg gtctgaacgg cacttgggcc ctcatcatcc agcgccgcct
      181 gagcgatcac ctgcaacttg acctcacgta caacggcaga cacagcctgg gcgtgcccat
      241 cacccatgtg ggaggggtgc aggtacgggc gtatttctga cgaaccgcgt gctatcgggc
      301 attcacccct gcacgtacac ccttcgaacg cgtgggctga tgcttgtgag tgcctcgtag
      361 ggaatggtac caaggtctcg ggcgtatttc tgcacgggat gctctggtcc gaaaacgatc
      421 acctcatcat cgatgtccac gggaatatcg gtgacatcca ccatgcacat gtccatgcaa
      481 atagtaccga cgaacggtgc ggctttgtcg ttgatccaca ccttgcccac agcgttgccg
      541 agcctgcggg ccaagccgtc cgcgtagcca atgggcaggg tggcgatgcg tgtttcccgc
      601 tgggcgatga agcgcctgcc gtagctcacg ctttcaccgg gcgccacggt ccgcagttgt
      661 gcgataacgg tgcgcagggt tgcaaccggc atcaattggc gcgtctcgcg ctcgtccaca
      721 ccgacgccgt gcaggccgat gcccagccgc accatgtcga gccgggcctc cgggaagcgc
      781 gacaccgcgc cgctgttcgc gatgtgccac agcgggcgat acccgagcac ttcgccgatg
      841 cgagtggcca tgctgctgaa ggcggcgatc tgcccgcgcg tgaagtcgtc ctgtgcggga
      901 tcctcgctgg cggcgagatg gctgaggatg gacgccacgt gcggcatgcg cttatcgcgg
      961 agttgttcca gcaaccaggg catgtccgct gccgtgaacc cgagccggtg catgcccgtg
     1021 tccagtttga tgtggatcgt tgtgctgctg cccgaagacg ccgcatgttc caggaccgcg
     1081 gccagcgaac gctcgtcgta tacctcgggc tccagatgga accgttggag cgtttccagc
     1141 ggtatcggct cgggattcat caccaggatg ggcacggtga tgccgtgccg acgcaattca
     1201 accccctcat ccgcgtaggc tacgcccagg tagtggatgc gctcgtggtc gaacaaccgg
     1261 gccaattcca acgccccgca gccgtatccg gccgctttca ccatggccat caggcgtata
     1321 tcgggcccaa cgatcgcgcg gtagtggttc aagttgtggc gtatcgcgcc gaggtccacc
     1381 tccagttcgg tgccgtgcga gtgctgctgc cagcggcgga ccacgcgctc caggcggaag
     1441 tcgcgtgcgc ctttcaccag caccacggcg cccatgagcg ccttcggttc ctcgttgcgc
     1501 agcagggcat cggcgtccac atggaagtgc gagcccggcg ggaaatgatg ccgatgtgcg
     1561 gagatcgacg gccccacgcc gatcacgtgg tccactttcg ccgcggacag gtcatgcgcc
     1621 acgcgttggt acaacagatc agccggaagg cccgatccca acatgtcgct cagtaccacc
     1681 acgcgccgac ggccgtgcgc catgtgcgcc aagtggtcca aggcgatacg caacgaggtg
     1741 gggtcgttgc tgtagctgtc gtcgatgagc gtacttccct gcgcgccttc caccgaacgc
     1801 atgcgcatgt caacggggct tagctggggc aaggcctcgt tgatccaatg agggtcacgg
     1861 ccaagctgca gcaataatgt gatgcaggtg agcgcgttct cccggctggc ggcgtcggtg
     1921 aacgggacga ggtattcgtg ccgcttcccc gcgtgcaaca gggtgatcgc cgttgcttcc
     1981 ccgcgttcca cctcgttcag cacgcgcacg aaggcatcgc gctcatgcga ccagtcccgc
     2041 aggacctctg gccgcagcac ggcttgcgca acgctgcgca ccacggtatt gtccgttccg
     2101 agcaccaccg catgcgcatc cttgaacaag cggagctttt cagcgcactt ctcatggtca
     2161 tccgtgaagc cgtcgccatg ggccgcaccg atgttggtga gcacaccgat cgtgggccgg
     2221 atcaccgggc gcaaacgatc catctcgccg gcggtgctga tgcccgcttc gaagaggccg
     2281 agcgtgtgcg cggggcccat ctccagcacg ctcaggggca cgccgacctg gctgttccag
     2341 ctgcccgggc tgcggacgat aagttcctcg gaacgcagca tctgccagag ccattctttc
     2401 actaccgttt tcccattgct accggtgatg ccgacgacgg gaaggttgaa atgcgcacgg
     2461 tgccatgccg cgaaacgctg cagggccagc agcgtgtccg ggaccacgat caggtcggac
     2521 cgcgcgaatg cggccgcccg cccctcttcc accatgaagg cccgtaaccc ctggagcgcg
     2581 agcccggcga tgtgatcatg gccatcgtgt cttggtccgc gcagggcgat gaacaggctg
     2641 gtggcggaac agctcccccg tgagtcgata gatacgctct ccaccatgcg cgcaccatcc
     2701 cccaggaggc ggccgcccac cgcctcggcg atcctggaaa gcgagtagca ccaggcgctc
     2761 atgtcccggg ctccatttca gctcgggccg cgaagaagca cgtgcggcac agcggctcgt
     2821 actggtcggt ctcgccgagc accaccagtt cgcgacttgt ggaggtccgg tggctgaaag
     2881 tggccagctc gccgcagcgc atgcagatgg cgtggacctt ggtgacatac tcagccatgg
     2941 ccatcagctg cggcatgggg ccgaaaggac gaccgaggaa atccatgtcg agccccgcag
     3001 cgatcacgcg aacaccttgg gaggcgagtt gctcgcacac ttgcggaaga ccggcatcga
     3061 agaactgggc ctcgtcgatg cccaccacct cgatatcgcc cgcgagcagg aggaggttgc
     3121 tgctgttggg cacgggtgtg ctgcggatgc tgttgcgatc gtggctcacc acatcctgcg
     3181 catcgtaacg ggtgtccacg ccgggcttga agatctccac gcgctgcttg gcgaactcgg
     3241 cccgacgcat gcgccggatg agttcctcgg tcttgccgct gaacatgctg ccgcagacca
     3301 cctcgatcca acccttgcgt tgggcgcggc tggcagcgcg ttcgaggaac atcggcggtg
     3361 gtcgggtggg gggcgaaaat agcccggccc ctctgctgga acagcgtcgt tcacggtcgg
     3421 ttgtcgatag gcgggtccaa ggaggggtgc gaggggtgtg ccctgggagt agtttcgtcc
     3481 ggcagatgag cgagaacaca ccggctggcc gcatgcacgg ccttgtggag aacctgttgc
     3541 gcgcgttgcg ggagctggac ggcggtcgtc tcgatcggga cgggttggag gtggcttgtg
     3601 ccgacgcgca ggccctgtat gaacggctca tcgtgctgcg tcacaaggca cgggagggag
     3661 ctcgtgccgt atcgcctgcc gcacctgagg aggcagcggc ttccccgggg ggcgggatcg
     3721 tggccacgtc ggaaatgccg ccgttgcggc tggaaacccg acctgccgaa gtacccccgc
     3781 ggcagacctc attgatcgat gcgatcgcgg aatccgagac ccccgccact ccgaccgtgc
     3841 cgccgcaccc aacgccgaag gccgaaccgc cccaacgcgt acgaggcacc tcgcaaccac
     3901 cccggaaacc ggaacgcacc gggccgggtc ataccgaaac acaaggatca accttgggcg
     3961 acaagctcga acgcgcacct gttgccgatc tgcacaaggc gatcgccctg agccagaagt
     4021 tctggttcgt tgcggagctg ttcggtgggc accgcgacag gtacgagcag gccgtgaacg
     4081 ccctcaatgc gatgggttcg ctggacgagg cacgcacctt catcgccaac gagatcacgg
     4141 ccaaggccac ccgaccaccg gccgcggatg ccatccaagc attcaacgac cttgtggaac
     4201 gccgcttcaa gtgatcccgg cacgaaccct gcacaccttg gacatgctcc gatccatcgc
     4261 ttcgctcctg accatgggcc tcctgctggg ggccagcgct caatcgccag cttccgtgaa
     4321 ggaaaaggcc cgggcctatg tgaacgtcct ggccgggccg accatgcacg gccgcggtta
     4381 cgtgctcgaa ggcgaccgga tcgccgcgga ctggatcgcg gcacagttcg atcgattggg
     4441 gctgggcaag ttgaacgacc aacgattcga gccgttcacc ttcgccgtga acaccttccc
     4501 cgatacggtg cgggcggcca ttgatggtcg tgcgcttgtt cccggtgtcg atttcctggt
     4561 ggacc
//
LOCUS       DAOQOC010000081         4557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8251_length_4557_cov_4.741004, whole genome shotgun sequence.
ACCESSION   DAOQOC010000081 DAOQOC010000000
VERSION     DAOQOC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8251_length_4557_cov_4.741004"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PL106_02125"
     CDS             complement(<1..695)
                     /locus_tag="PL106_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA31895.1"
                     /translation="MRRILIISSLLNSALALAGVPDSTEAILRTEGRTTRRVDALLRM
                     GFATYESPAAENTVYWREAQSISEELRYKVGLVHAPMEAVMIDFRAGRYDAVVGRLKI
                     MIDRLDALGVDQDHYTPLGLLRNTFNASGDQAARFAYFSEALTRYSNSDRPKSLATTH
                     HALAGYYRTIRQYGSAVEHYMKARDLFRTCSPAEVPNETMVIGWAYEQWGNPQRAIPF
                     LEAALREYEAQGD"
     gene            746..2449
                     /locus_tag="PL106_02130"
     CDS             746..2449
                     /locus_tag="PL106_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31896.1"
                     /translation="MAPYSWGFRTDRSYERLQPSLYRMRILITLLLISCSLTVLAQRP
                     APSPYGTPAERLSAGSAERGGPPANDDCANATTITPGADCAGALIGTNAGATMDGAVP
                     SCDDPGATEPDVWYTFTTGTADTMIITLTPQAGMTDWAYVLLESTCGGSEVACRIQPS
                     IPTAEYLTPSTTYYLRVFSNPDYGDAGDFELCLQDLALVNVPANDLCTNAPMQALPMG
                     TTITFTGNSTNAQNTEGLPLSSVWHAFTLSEAADVTIDLCGSAAFFPYYFRALYFTCP
                     ADLTQRRYAGYENITDCTGNRPTLCYPALPAGTYYYAVANGDAPGTYTLHVKAEPLGS
                     HQPTNDDCAGAIAVPVSATCAPVAFSPTCASASTVQAGCADGLGDASDDVWYSFVATQ
                     SLMTIGVFPNSTQFGAVMELYTGVCGALVSTACANGFDGDPVQLPLNGMVPGNTYYVR
                     VYNGYSNTPYDDAGYSLCVAEGFGIAIGIEEAAGGLPTLDVFPNPAAESFSIRTGSPG
                     STFSLAVIDAAGRTVINTTVRADASGTVQLAEGGMLAKGLYTVKVSDPRSARSARLVI
                     H"
     gene            2469..>4557
                     /locus_tag="PL106_02135"
     CDS             2469..>4557
                     /locus_tag="PL106_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNA31897.1"
                     /translation="MQRLLSLVLIALPLHVLAQPRMSATTQRAIAVLEQLAKEQPDAR
                     KLTEQAQTRFPVMYIAGRCMVGFLGRATVAETIADEPISVGARAGNIVSFRVDAHHLA
                     SVRSITAFDQVEIAQRRRPELNQVIPDIRADSVHLGIGLPQGYTGHDVLLGISDVGFD
                     FTHPAFYDTAMSATRIVAAWDQFKQSGPPPAGYGYGAAYTTAAELIAAEGDTATYGGR
                     WTHGTHVAGIAGGAGAGTPYRGVAYGAGLLFVTLNDDAGILDGFAWMNEVAQQQQKRL
                     VINMSWSSIDLQDGTSLFCQAIDALADQGIVFCAANGNNGGIPFHIQRAFVGDTLRTR
                     IAFPVFSDPGFDWRGIILLSQPGAPFEGRILIRDASNTIIGQTPWMSTATQAAYTDTL
                     MVFGTDTVELRILADAAHPQNQRPHLRVHVRKTSTLLRVDLNVTAPGGTVHGWNAEST
                     GDFAGIWGEPFQAAMPGYAAGDLEYGVGEPACGNGVLAVGAYRAAYQDGGNWLGGEIG
                     DFSVKGPTLDGRLKPDITAPGVHVVSCVSSYTTESVTPVAVLVFQGRGYMFASWEGTS
                     MSSPVGAGVAALLLEAFPFATPAQVRSAIMENARTDAFTGPIPPNGSTLWGMGKINAY
                     HAVTALMPFAGVADMEDQGTRIWPNPADEVLHVALEGPLDHARFAVLDLTGRLVSEGS
                     LNQASPTIDVRSLV"
BASE COUNT          786 a         1481 c         1439 g          851 t
ORIGIN      
        1 ctatccccct gagcctcgta ttcgcgcagc gctgcctcca ggaagggaat ggcgcgctgc
       61 ggattgcccc actgctcata ggcccagccg atcaccatgg tctcattggg aacctccgca
      121 ggcgagcaag tgcggaagag gtcacgcgcc ttcatgtagt gctccacggc cgaaccgtac
      181 tggcggatcg tgcggtagta accggcgagc gcgtggtgcg tggtggcgag gctcttggga
      241 cggtcgctat ttgaatagcg cgtgagcgct tcactgaaat aggcgaagcg ggcggcctgg
      301 tcaccggagg cgttgaacgt gttgcgtagg agaccgagcg gtgtgtaatg atcctggtcc
      361 actccgagcg catcaaggcg gtcgatcatg atcttcaacc ggcccaccac cgcgtcatag
      421 cgcccggccc tgaagtcgat catcaccgct tccatgggcg catgcaccag acccaccttg
      481 tagcgcagtt cctcggagat gctttgtgct tcgcgccagt agaccgtgtt ctccgccgcg
      541 ggtgattcat acgtggcgaa acccatgcgc aacagggcgt ccacacggcg cgtggtgcgg
      601 ccctcggtgc gcaggatggc ttccgtgctg tccggaacac cggcgagcgc gagcgcggag
      661 ttgaggaggc tgctgatgat gaggatcctg cgcatcgtgg ggcgaaacaa tcgctgtgcg
      721 ggcgtgtgcg cgcccccatg atcgggtggc cccctattca tggggtttcc gcacggaccg
      781 ctcttatgaa cgtttgcagc ccagcctcta tcgcatgcgc atcctcatca ccctcctcct
      841 gatctcctgc agcctgacag ttctggcgca acgaccagct ccgtcaccct atggtacgcc
      901 ggccgaacgg ctttcggcgg gcagcgctga gcgcggtggg ccacccgcca acgatgattg
      961 cgccaatgcc acaacgatca cgcctggtgc cgattgcgcc ggggcattga tcggcacgaa
     1021 tgcaggcgcc accatggacg gcgctgtgcc atcctgtgat gatccgggag ccacggaacc
     1081 tgatgtatgg tacacgttca ccaccggcac ggccgataca atgatcatca cgttgacccc
     1141 gcaggcaggc atgacggatt gggcgtatgt actgcttgag tcaacatgcg gtggtagcga
     1201 agtggcctgc cggatccagc cttcgatccc tacggctgag tacctcacgc cttccaccac
     1261 ctactacttg cgtgtcttct ccaatcccga ctacggcgat gcaggcgatt tcgagctgtg
     1321 ccttcaggac cttgctctgg tgaatgttcc cgcgaacgat ctgtgcacga acgcgccgat
     1381 gcaagccctg cccatgggca ccaccatcac cttcaccggc aattccacca acgcgcagaa
     1441 caccgagggc ctgccgctgt cttcggtgtg gcatgccttc accttgagcg aggcggccga
     1501 tgtgacgatc gacctctgcg ggagcgcggc cttcttcccg tactacttcc gcgcactgta
     1561 cttcacctgc ccggcagatc tgacccagcg ccgctacgcg ggttacgaga acatcaccga
     1621 ctgcacgggg aacaggccca cgttgtgcta tcccgctttg ccggcgggca cctattacta
     1681 tgccgtggcc aacggcgatg cgcccgggac ctacaccctg catgtgaagg ccgagcccct
     1741 gggttcgcac caacctacga acgacgactg tgcgggtgcc atcgctgtgc cggtctctgc
     1801 gacctgcgct cccgtggctt tctcgcccac ctgcgcctcg gcctccaccg tgcaagcggg
     1861 ctgtgccgat gggctcggcg atgcctccga cgatgtctgg tactccttcg tcgccacgca
     1921 gtcgttgatg accatcggcg tgttcccgaa cagcacgcag ttcggcgccg tcatggaatt
     1981 gtacacgggc gtttgcggcg ctctggtctc cacggcctgc gccaatggtt tcgatggtga
     2041 cccggtgcaa ctccccttga acgggatggt acccgggaac acctactacg tgcgagtgta
     2101 caatggctac tccaacacgc cctacgatga tgcgggctat tcgctttgcg tggccgaggg
     2161 cttcggcatc gcgatcggga tcgaagaagc cgctgggggc ctgcccacgc tcgacgtgtt
     2221 ccccaacccg gccgcagagt cgttcagcat ccgcaccgga tcgccaggtt ccaccttctc
     2281 gctggccgtg atcgatgccg caggaaggac cgtgatcaac acgaccgtgc gtgccgatgc
     2341 cagtgggacc gtgcaactgg cggaaggtgg gatgctggcg aaaggtcttt acacggtgaa
     2401 ggtgagcgat ccgcggagcg ctcgttcggc ccgtttggtc atccattgat caccagcatc
     2461 cgatgcccat gcagcgcctc ctgtcactgg tgctcatcgc gcttcccctg catgtgctgg
     2521 cacagccgcg catgtcggcc accacgcagc gcgcgatcgc ggtcctggag cagctcgcca
     2581 aggagcaacc tgatgcccgc aagctgaccg aacaggcgca gacccggttc ccggtgatgt
     2641 acatcgctgg acgttgcatg gtcggtttcc tcggccgtgc cacggttgcg gagaccatcg
     2701 cggacgagcc tatcagcgtt ggcgcgcgcg ctggcaacat cgtctccttc cgggtggatg
     2761 cgcatcacct ggccagcgtg cgttcgatca ccgcgttcga tcaggtggag atcgcgcagc
     2821 gcagacgtcc ggagttgaac caggtgatcc cggacatccg cgccgatagc gtacatctag
     2881 gcatcggtct gccacagggc tacaccggcc atgatgtgct gctggggatc agcgatgtcg
     2941 gcttcgactt cacccacccg gcgttctacg acacggccat gagcgccacg cgcatcgtcg
     3001 ccgcgtggga ccagttcaag cagagcggtc cgccgccggc tggctacggc tatggcgcgg
     3061 cctacaccac agcggccgag ctcatcgccg ccgaaggcga taccgccacc tacggtgggc
     3121 gctggacaca cggcacgcac gtggccggta tcgccggagg ggccggcgca ggcacgccgt
     3181 accggggcgt cgcgtacggc gcgggattgc tgttcgtcac actgaacgat gatgccggca
     3241 tcctcgatgg cttcgcctgg atgaacgagg tcgcacagca gcagcagaag cggctcgtga
     3301 tcaacatgag ctggtcgtcg attgaccttc aggatggcac ctcgctcttc tgccaggcca
     3361 tcgatgcgtt agcggaccag ggcattgtct tctgcgctgc caacggcaac aacggtggca
     3421 ttcctttcca catccagcgc gcgttcgtgg gcgatacgtt gcgcacgcgc atcgccttcc
     3481 cggtgttcag cgaccccggc ttcgactggc gcggcatcat cctcttgagc cagccggggg
     3541 cgccgttcga agggcgcatc cttatccgcg atgcgagcaa caccatcatc ggtcaaacgc
     3601 cctggatgtc cacggccacg caagccgcat acaccgatac gctcatggtc ttcgggacgg
     3661 ataccgtgga gctccgcata ctcgctgatg ccgcacatcc gcagaaccag cgcccgcacc
     3721 tgcgggtgca tgtgcgcaag acgagcacgc tcttgcgtgt ggacctgaac gtgaccgcgc
     3781 cgggcggcac ggtgcacgga tggaacgctg agtccaccgg cgacttcgcg ggcatctggg
     3841 gcgagccctt ccaggccgcg atgcccggtt acgcagcagg cgatctcgaa tacggtgtgg
     3901 gcgaacctgc gtgtggcaac ggtgtgcttg ccgtgggcgc ctaccgcgca gcttaccagg
     3961 acggcggcaa ttggctcggt ggcgagatcg gcgacttctc ggtgaaaggg cccacgctgg
     4021 atggtcgcct gaagcccgac atcaccgcgc ccggcgtgca cgtggtgtcc tgcgtgagct
     4081 cgtacaccac ggagtcggtg acccctgtgg ccgtgctcgt ctttcaaggt cgtggctaca
     4141 tgttcgcgag ctgggagggc accagcatga gcagccctgt gggagcggga gtggcggctt
     4201 tgctgttgga ggcgttcccc tttgccactc cggcgcaagt gcgatcggcc atcatggaga
     4261 acgcgcgcac tgacgcattc accggaccga taccgccgaa cgggagcacg ctctggggta
     4321 tgggcaagat caacgcctac catgcggtca ccgcgttgat gcccttcgcc ggtgtagcgg
     4381 acatggagga ccaggggaca cgtatatggc ccaacccggc tgatgaagtc ttgcatgttg
     4441 cgctagaggg gccattggac cacgcgcggt tcgccgtgct ggacctgacc ggtcggctgg
     4501 tgagcgaagg gagtttgaac caggcgagtc cgaccatcga tgtgcgatcg ctcgtac
//
LOCUS       DAOQOC010000082         4532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8323_length_4532_cov_4.067233, whole genome shotgun sequence.
ACCESSION   DAOQOC010000082 DAOQOC010000000
VERSION     DAOQOC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8323_length_4532_cov_4.067233"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PL106_02140"
     CDS             complement(<1..918)
                     /locus_tag="PL106_02140"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA31898.1"
                     /translation="MPCRVMGRPGTSGSPVSKMAGAAFKRILFLILTWSTQGSITAQP
                     NPNPDPTLDASIVNELSAQHFPGAVTVIVKDDRIVWMQGYGLADIANAIPVTDTTNFI
                     LASVSKLFTGTAAMQLIENDVIDLDDDVDDFLPWPVHVPGFPNDPITFRQLMTHSSSI
                     QDDYGTMGTYYGYPDPTMTLADCMQRYFSPTGADFDPADNFIATQPGSVFEYSNMATA
                     LNGYLVEHASGMGFDPYCQTHIFEDLCMERTYWHIAYHDTTGIARPYRWSGGNYIPYA
                     HYGFADYPDGQLRSNARDLANFMIAYLNGG"
     gene            1130..3229
                     /gene="metG"
                     /locus_tag="PL106_02145"
     CDS             1130..3229
                     /gene="metG"
                     /locus_tag="PL106_02145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNA31899.1"
                     /translation="MRPVARTTVTAALPYANGPLHIGHIAGAYLPADIYVRCLRARGK
                     EVLFVCGSDEHGAAITLRALKEGVTPRAIVDKYHALMGKAFQEFGIHFDIYHRTSSDL
                     HRATSQEFFLQLDRNGAFTEQEEQQYFDEEARQFLADRYIKGTCPNCGNPDAYGDQCE
                     KCGTALSPKDLKEPRSTLSGSKPVLRSTKHWYLPMDRSQAWMEQWINTGMLDGVQQHD
                     AGEWKPQVLGQCNSWLKDGLRPRAMTRDLDWGVPVPLPGADGKVLYVWLDAPIGYISA
                     TKQWAQDHASAGSASDWEKWWKSDDTRLLHFIGKDNIVFHCIIFPMLLKLHGGYILPQ
                     NVPANEFLNLEGQKLSTSRNWAVWLHEYMERWPGRQDELRYCLGSILPEFKDSEFTWK
                     DFQEKNNSELVSIIGNFVQRVFVLCHKYYDGRVPEPAGGDESDVVLWAGLSAMPDRVA
                     AEVDRFRFRDALANAIDVARMGNKYLSDNEPWKLERTDPARVRTILYNCLRIVGGLSV
                     VLEPFLPFTAQRMRTMLGLGELKWEDALRADLIRPGSPLAKPEHLFKPITDAEIQPEI
                     DRLHATRTTAPSTGALTAPPPASKPDIGFDDFAKLNLRTGTIVAAEKIPKADKLLKLT
                     IDIGEPMPRTVVSGIAQHYSPEQVVGQAVVLVANLEPRKMRGVESQGMVLMTEDATGR
                     LVFVRPQDAVTPGSEVR"
     gene            3342..3416
                     /locus_tag="PL106_02150"
     tRNA            3342..3416
                     /locus_tag="PL106_02150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3375..3377,aa:Arg,seq:cct)
     gene            3554..>4532
                     /locus_tag="PL106_02155"
     CDS             3554..>4532
                     /locus_tag="PL106_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA31900.1"
                     /translation="MGSFVRALPPVLVALLLLSTSCLLAQPANSDCDDATTVCAGTPG
                     SGNNTGANIWPGFCTTPPTEHVVWYTFTTNSQGGPVEVSISDIACPPVAGMDNELSAV
                     VFSGDGNCTAGAFTSVGDCQHDSVDFTITTNALSPQTQYWVVVAGVLDGSATLAAECG
                     FSIEVGGPGADIIGVDFSAGEDVTVPEGANTQLLATGGTTYNWSPLAGLSGNGIPDPI
                     AQPEETTSYTVTTQIGPCSYTDTVVISVERPLTPPNTITPNGDGINDTWEIPGIHDHP
                     QATVTIYDRWGQRVFKDTGYKTPFDGKDLPSGTYYWVIELSRLEGTSKPI"
BASE COUNT          876 a         1373 c         1482 g          801 t
ORIGIN      
        1 cccaccgttc aggtatgcga tcatgaagtt ggcgagatcc ctggcgttgc tgcggagctg
       61 accatcgggg taatcggcga aaccgtaatg ggcatacggg atgtaattgc cgcccgacca
      121 gcggtacggc cgcgcgatcc cggtcgtgtc gtggtacgcg atgtgccagt acgtccgttc
      181 catgcacagg tcttcaaaaa tatgcgtttg gcagtatggg tcgaagccca tgccgctcgc
      241 gtgttccacc aggtagccgt tcagggcggt agccatgttc gagtattcga acacgcttcc
      301 gggctgggtg gcgatgaagt tgtccgcggg gtcgaaatcg gcgccggtgg gggagaagta
      361 gcgctgcatg cagtccgcca gcgtcatggt ggggtcgggg tacccgtagt acgtacccat
      421 ggtgccgtag tcgtcctgga tggaggagga atgggtcatc aactggcgaa aggtgatggg
      481 gtcgttcggg aagccgggta catgtacggg ccatggaagg aagtcatcaa catcgtcgtc
      541 caggtcgatc acatcattct cgatcaactg catggccgct gtgccggtga acagtttgga
      601 gacggaggcc aggatgaaat tcgtggtatc ggtcaccggg atcgcgttgg cgatgtcggc
      661 caacccatag ccctgcatcc aaacgatccg gtcgtccttc acgatcacgg tgaccgcccc
      721 ggggaagtgt tgcgcgctca gctcgttcac aatggacgca tcgagggttg gatcggggtt
      781 cgggttgggt tgggccgtta tggaaccttg ggttgaccac gtcaggatca ggaacaggat
      841 gcgtttgaac gcggcgccag ccattttgct gaccggggat ccgctggtgc ctggcctccc
      901 catcacgcgg caaggcatgg tcagatcggt cggtagcgat atccgcggcg ctgccgatgt
      961 gcatggcccg ttggccgggc gggtcaggaa tatcaccatg ggcatcgctc aaagttagcc
     1021 ggcatcacgg aggggggatg tgaaggatcc tgcaagggac ccgcgaagga cacatgcacg
     1081 tccaaggcga caggtgcgaa cggtgggagc cggtacattc gcgcgccgca tgcgacccgt
     1141 tgcccgcacc accgttaccg ccgcactccc gtacgccaac ggccccttgc acatcgggca
     1201 cattgccggg gcctacctgc cggccgacat ctatgtgcgg tgcctgcgtg cgcgtggcaa
     1261 ggaggtcctc ttcgtgtgcg gcagcgacga gcatggtgcg gccatcacgc tccgagcact
     1321 gaaggagggg gtgacaccac gcgccatcgt ggacaagtac catgcgttga tgggcaaggc
     1381 ctttcaggaa ttcggtatcc atttcgacat ttatcaccgc acgagcagtg atcttcaccg
     1441 cgccaccagc caggaattct tcctgcagct ggaccgcaac ggcgccttca ccgagcagga
     1501 ggagcagcag tacttcgatg aggaggcccg tcaattcctc gcggaccggt acatcaaagg
     1561 gacctgcccc aactgcggca accccgatgc gtacggcgac cagtgtgaga agtgcggcac
     1621 ggccctgagt ccgaaggacc tgaaggagcc gcgcagcacc ttgagcggga gcaagcccgt
     1681 attgcggtcc accaagcatt ggtacctgcc catggaccgc agccaggcct ggatggagca
     1741 gtggatcaac accggcatgc tcgatggcgt gcaacagcac gatgcggggg agtggaaacc
     1801 gcaggtactg ggccagtgca acagctggct gaaggacggg ctgcggcccc gggccatgac
     1861 gcgcgatctc gattggggtg tgcccgtgcc cctgccgggg gccgatggca aggtgctgta
     1921 cgtgtggctc gatgcgccca tcggttacat ctcggccacc aaacaatggg cgcaggacca
     1981 cgcatcagcg ggctcagcgt ccgactggga gaagtggtgg aagtcggatg acacgcgtct
     2041 cctgcacttc atcggcaagg acaacatcgt gttccattgc atcatcttcc cgatgttgtt
     2101 gaagctgcac ggcggttaca tcctgccgca gaacgtaccg gccaacgagt tcctcaacct
     2161 cgaaggccag aaactcagca cgagccggaa ctgggccgta tggctgcacg agtacatgga
     2221 gcgttggccc ggtcggcagg atgagttgcg gtactgcctg ggcagcatcc tgcccgagtt
     2281 caaggacagc gagttcacat ggaaggactt ccaggagaag aacaacagtg aactggtgag
     2341 catcatcggg aatttcgtac agcgtgtttt tgtactttgc cataagtatt acgacgggcg
     2401 cgtaccggaa cccgccggcg gcgatgagag cgacgtggtg ctctgggccg gcttgagcgc
     2461 catgcccgac cgggtggccg ccgaggtgga ccggttccgc ttccgcgatg cgttggccaa
     2521 tgccatcgac gttgcccgca tgggcaacaa gtacctttcg gacaacgagc cgtggaagct
     2581 ggagcgcacc gatccggccc gggtgcgcac catcctgtac aactgcctgc ggatcgtggg
     2641 cggcctcagc gtggtgctgg aacccttcct gccgttcacc gcgcagcgga tgcgtaccat
     2701 gctgggcctg ggcgagctga agtgggagga tgccttgcgc gcggacctca tcaggccggg
     2761 gtcgccgctc gccaagccgg agcacctctt caagcccatc accgatgcgg agatccagcc
     2821 cgagatcgac cgcttgcacg ccaccaggac gaccgcccca tcgacgggag ccctgacggc
     2881 accgcctccg gcgtcgaagc cggacatcgg cttcgatgac ttcgcgaagc tcaacctgcg
     2941 caccggcacg atcgtggccg ctgaaaaaat acccaaggcc gataagctgc tcaagctcac
     3001 catcgacatc ggggagccga tgccgcgaac ggtggtgagc ggtatcgcgc agcactattc
     3061 gcccgagcag gtggtggggc aggccgtggt gctggtggcc aacctggaac cccggaagat
     3121 gcggggcgtg gagagccagg gcatggtgct gatgacggag gacgccacgg gcaggttggt
     3181 gttcgttcgg ccgcaggatg cggtgacccc cggctccgaa gtgcgctgag ccaccgtgcg
     3241 cagtgcgggc gctgcacccc gaactcttcc cccggtgttc cggttgcgcc cgtgacctct
     3301 actttcgccg cccgttcttt cggcccgcgg tccggcaaca ggaccccata gttcaatgga
     3361 tagaatagga gtttcctaaa ctcttgatcc aggttcgact cctggtgggg tcaccaaccg
     3421 tgtttttcgt tgccgtagcg ggccgtgtgg cctggagccg gtcaaaaccc gcaccgggtg
     3481 aacaaggtcg tgtgagaaaa aggagggcga agcgtcgggg ccacaggagg atagccgcaa
     3541 ctttcggcga cccatgggct ccttcgtccg cgcacttccg ccagtactgg tggcgctgct
     3601 cttgctgagc acgtcatgtt tgttggcgca gccggccaac agcgactgcg acgatgccac
     3661 cacggtctgc gcgggtacgc cgggctcggg caacaacacc ggggcgaaca tctggccggg
     3721 cttctgcacc acgcccccca cagagcacgt ggtgtggtac accttcacca cgaacagcca
     3781 aggtggtccg gtggaggtat ccatctcgga catcgcgtgc ccaccggtgg cgggcatgga
     3841 caacgagctg agcgccgtgg tcttcagtgg cgatggcaat tgcacggccg gcgcgttcac
     3901 atcggtcggc gattgccagc acgattccgt cgacttcacc atcaccacga atgcattgtc
     3961 gccccagacc cagtattggg tggtggtggc aggagttctc gatggcagcg ccacgctggc
     4021 cgcggaatgc ggcttcagca ttgaagtagg cgggccgggc gcggacatca tcggcgtgga
     4081 tttcagtgcc ggcgaggacg ttaccgtgcc ggaaggcgcc aacacccagt tgctggccac
     4141 cggaggcacc acgtacaact ggtcgccact ggcggggctc agcggcaacg gcatccccga
     4201 tcccatcgct cagccggagg agaccaccag ctacaccgtg accacgcaga tcggcccttg
     4261 ctcgtatacc gatacggtgg tcatttcggt ggaacgtccg ctcaccccgc ccaacaccat
     4321 caccccgaac ggcgacggca tcaacgatac gtgggagatc ccgggcatcc acgaccaccc
     4381 acaggccacg gtcaccatct acgatcgctg gggccagcgt gtgttcaagg acacgggata
     4441 caaaacaccg ttcgatggga aggacctgcc ctcgggcacc tattactggg tgatcgaatt
     4501 gagccgcctg gagggcacct cgaaacccat ca
//
LOCUS       DAOQOC010000083         4514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8378_length_4514_cov_3.799058, whole genome shotgun sequence.
ACCESSION   DAOQOC010000083 DAOQOC010000000
VERSION     DAOQOC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8378_length_4514_cov_3.799058"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PL106_02160"
     CDS             complement(<1..483)
                     /locus_tag="PL106_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNA31901.1"
                     /translation="MSTKRPIQALHPATQSRDRDARIVATLERLGEVLRNLSWDAGRS
                     IKASHLHVQMLAFLRGRSKEGTRGVDLAARFHLSKPTVSVAVRSLEEHGAVRLVTSKS
                     DSRAKVIVLTPKGRRMAEKATSHLDALVPLLERIPSDDRDALYASLFALLDAARTAGL
                     V"
     gene            complement(547..1113)
                     /locus_tag="PL106_02165"
     CDS             complement(547..1113)
                     /locus_tag="PL106_02165"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HNA31902.1"
                     /translation="MKGYDYGKVGPSPLTMKDLDALKATVLFGPDDEKYLRMAGDVLR
                     DQTDAVLDLWYGFVGGNPHLVHYFTHNGAPDPAYLAGVRARFGQWIMDLCNKPYDQVW
                     LDYQHEIALRHHSTKKNRTDGVQAVPIIHMRYLVAFIFPITFTIKGFLVKKGHSADDV
                     EKMYAAWFKAVTMTATLWCEPYVRNGEF"
     gene            1271..1534
                     /locus_tag="PL106_02170"
     CDS             1271..1534
                     /locus_tag="PL106_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HNA31903.1"
                     /translation="MEVAVWDTYVEKKDGTVMHFDIIAPSEQRDTLVIHNFGRDYLRL
                     KGQEGQPLTAKECRFCHVQEVRPQWQQEILAKGYFILEMEGCE"
     gene            complement(1998..2636)
                     /locus_tag="PL106_02175"
     CDS             complement(1998..2636)
                     /locus_tag="PL106_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA31904.1"
                     /translation="MKTDDRIRVAVVEDDEVLREAMVEALEPDPALKVVGAFADAEAF
                     NAAVHERPPTVVVMDLNLPGMSGIECTRRLKLHWPQVQVLVCTVQDDDDSLFNALCAG
                     ATGYLLKDARPAQVVEAVKTIASGGSPMSAGIARRVLASFNVPRKAAKEIDLLSQRER
                     QVLEELARGLRYKEIADTLNLSIDTVRTHVRNLYDKLQVGSRTDALNKLYPR"
     gene            complement(2633..>4514)
                     /locus_tag="PL106_02180"
     CDS             complement(2633..>4514)
                     /locus_tag="PL106_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNA31905.1"
                     /translation="AFHQGGLALFDERTDWLEPLVLPGSGANTSWSHIFNDPSGTIWA
                     RLDGHVYAIDQRDGRPMVHAPLPGPVALLRDVEGHALLYAENRALRVERRGAAWDTTT
                     MTLAFPDSLDRLAERLVMDPIGQWWTSGAVLPIGVWKDGVSLPLAGDVPDTGTLMTGF
                     FCTGTDTAWMTTSNGLYSWSLRDRRVLRHLTIHDGSPDQYLYMMLSDGDGSWWVSSNN
                     GLSHFDPQRGSFRNYTVPEGLQSREFNSDAAFRSASGRSYFGGVNGFNHFLPEAVRDD
                     PDTALVRVVGTFVGDSPVPFEAGITLPFRRNELRVELAVLEFTAPEGNRYAYRLAGYD
                     TTWRVQRADRPIMFTNLPSGSYSLLVKGINADGVESRELVLLAVQVPLPWWASPWAIA
                     AAIALLAGVVGYVVYAVQRARARRRLAAAEHELGELRQRTRLAKEIHDDVGSGLARIA
                     ALSRSTKREADAAERFDKLADISGELLLNLRDVVWMNDPRHDTLDALLLRISDHARDL
                     FEDSEALVEVRFPVPMPTHPVGGHFRHNLFLIAKEALHNAHKYSQAQRITLVWAQDAE
                     GFRFEVIDDGVGITNGGAVGGGHGSANMRQRAEDLGARYERISLPGAGTTIRVQGRTF
                     GP"
BASE COUNT          886 a         1436 c         1382 g          810 t
ORIGIN      
        1 caccaaccct gctgtgcgcg cggcatccag caaggcgaag agacttgcgt agagtgcgtc
       61 acgatcatca ctggggatcc gttcaagcag gggcaccaac gcatccagat gagatgtggc
      121 cttctcggcc atcctccggc ccttcggtgt gagaacgatc accttggcac ggctatcgga
      181 cttcgaggtc accaggcgta ccgcaccatg ttcctccaac gagcggaccg cgacgctcac
      241 tgtcggcttg gacaggtgga acctcgcggc gaggtccacc ccacgtgttc cttccttcga
      301 gcgaccacgc aggaaagcga gcatctgcac atggaggtgg ctcgccttta tggaccggcc
      361 cgcgtcccac gacaggttac gcagcacttc tcccaaacgc tccaatgtcg ccacgatacg
      421 cgcatcgcga tcccgggatt gggtggcggg atgcagtgct tgaatggggc gcttggtgga
      481 catggccgca aggtagcacg acggtgcggg ccaggtccct tgccgcagcc cgcaccggca
      541 gtgcgctcag aactcgccgt tcctcacgta cggctcgcac cagagcgtcg cggtcatcgt
      601 cacagccttg aaccacgcgg cgtacatctt ctccacatcg tcggcgctgt ggcccttctt
      661 gaccaggaaa cctttgatgg tgaacgtgat cgggaagatg aaggccacca ggtagcgcat
      721 gtggatgatg ggcacggcct gcacgccatc ggtcctgttc ttcttggtgc tgtggtggcg
      781 cagggcgatc tcgtgctggt agtcgagcca gacctggtcg tagggcttgt tgcacaggtc
      841 catgatccac tggccgaaac gcgcgcgcac accggcgagg tatgcgggat cgggcgcacc
      901 gttgtgcgtg aaatagtgga ccaggtgcgg gttgccgccc acgaagccgt accaaaggtc
      961 cagcaccgcg tcggtctggt cccgcagcac atcaccggcc atgcgcaggt acttctcatc
     1021 gtccggaccg aagagcacgg tggccttcag cgcatcgagg tccttcatcg tcagtgggga
     1081 agggcccact ttcccgtagt catatccttt catggggtgt gcggtttatg gcgcaagtat
     1141 agttcgtatt acttactatt gtgcggtgcg gaccgctgcg acattcatca ctctgctctg
     1201 cggagaactg cttgggccgg gtaccgcact tgcacaacaa gccaggatac aacaaccaac
     1261 aggaacgaaa atggaagtcg cggtctggga tacttacgtg gagaagaagg acggcacggt
     1321 gatgcacttc gacatcatcg ctccgagcga gcagcgcgat acgctggtga tccacaactt
     1381 cgggcgcgac tacctgcgcc tcaagggcca ggaagggcag ccgctcacgg cgaaggaatg
     1441 ccgcttctgc catgtgcaag aggtgaggcc gcaatggcag caggagatcc tggcgaaggg
     1501 ctacttcatc ctggagatgg aggggtgcga gtgatgtggg tcggctgccg cacctcccgt
     1561 gatccacggc ctgatgatcc gcgcactgcg acggccgata gcatcacgca ccatgagcac
     1621 gtgataccga ttcgcagcaa gggtgacccg aagatgcacc ggtatttttt cgcaggacgg
     1681 accatggaga ttgttgcgtg accggagctg tggaacgacc tgcacggtga agcagtgcgg
     1741 gaaatgcttc atcccgttcc gcaacagggg atcaggaccc gttccgccaa agcgggattg
     1801 gatcaagcat tggtccggga tggtagtact accatgtccc aaggggatcc ctgcatgtct
     1861 ccaagctgaa cggttgaccg cgatgcacct ctgccgatga actcacgata tccggagtga
     1921 tgaacccgga ccagcaggac cgcatgtaca gcatccagct ggaattctcg caggtgcgac
     1981 cagatccaac cccgaattca gcgcggatag agcttgttga gcgcatccgt gcgggagcct
     2041 acctgcagct tgtcatacaa gttccggacg tgagtgcgca ccgtgtcgat actcaggttg
     2101 agcgtgtccg cgatctcctt gtaccgcaga ccgcgggcca gttcctcaag cacctgtcgt
     2161 tcgcgttggc tcaacaggtc gatctccttg gcagccttgc ggggtacatt gaagctggcc
     2221 agcacccggc gcgcgatgcc tgcgctcatc ggcgagccac cactggcgat ggtcttcacc
     2281 gcctccacga cctgggcagg tcgggcatct ttgagcaggt agcccgtggc cccggcacat
     2341 aacgcattga acaggctgtc atcatcatcc tgcaccgtac aaaccaggac ctgtacctgc
     2401 ggccaatgga gtttcaaccg gcgcgtgcat tcgatgccgc tcatcccggg caggttcagg
     2461 tccatcacga ccaccgtggg tggccgctcg tgaacagccg cgttgaatgc ctccgcatcg
     2521 gcgaaggctc cgaccacttt cagcgccgga tcaggctcca gtgcctccac catggcttcg
     2581 cgcaacacct cgtcgtcctc caccaccgcc acgcggatcc gatcatcagt cttcatggcc
     2641 cgaaagttcg cccttgcacg cggatcgtgg taccggcacc cggcaagctg atgcgttcgt
     2701 agcgggcacc caggtcctcg gcgcgctggc gcatgttggc cgagccgtgc ccgcctccca
     2761 ccgcgccccc attggtgatg cccacgccgt catcgatcac ctcgaaacgg aatccttccg
     2821 cgtcctgcgc ccacaccagg gtgatgcgtt gcgcttggct gtacttgtgc gcgttgtgca
     2881 acgcctcctt ggcgatcagg aagaggttgt ggcggaagtg gccacctacc ggatgcgtcg
     2941 gcatcggaac ggggaaacgc acctccacca gggcctcact gtcctcgaac aggtcgcgcg
     3001 cgtgatcgct gatccgcaac aacagggcat cgagggtatc gtgccgggga tcgttcatcc
     3061 acaccacatc gcgcaggttg agcaacagtt cgccgctgat gtccgccaac ttgtcgaagc
     3121 gttcagccgc gtccgcttcg cgcttggtgg aacgcgagag tgcggcgatg cgggccaggc
     3181 cgctgcccac atcgtcgtgg atctccttgg ccagccgtgt acgctggcgc aattcgccca
     3241 gttcgtgctc agccgcggcc aacctgcggc gcgcgcgcgc gcgttgcacg gcatacacca
     3301 catagcccac caccccggcc agcagggcga tggcggcggc aatggcccat ggggaggccc
     3361 accagggcag cggcacctgg acggcgagca gcaccagctc gcgactctcc acaccatcgg
     3421 cattgatacc tttcaccagg agcgaataac taccactcgg aaggttggtg aacatgatgg
     3481 gccggtcggc ccgttgcaca cgccaggtcg tgtcgtatcc tgccagacgg tacgcgtagc
     3541 ggttgccttc cggagcggtg aactccagga cggccagctc cacgcgcaac tcattccgcc
     3601 ggaagggcag cgtgatgccg gcctcgaacg gcaccgggga gtcacccacg aacgtgccca
     3661 ccacgcgcac caaggccgta tcaggatcat cccgcacggc ttccggcaga aagtggttga
     3721 agccattgac cccaccgaag tacgatcgcc cgctggccga acggaatgcc gcatcggagt
     3781 tgaattcccg gctctgaagg ccctcgggta cggtgtaatt gcggaaagaa ccccgttgcg
     3841 gatcgaagtg cgacaatccg ttgttgctac tgacccacca ggatccatca ccgtcggaca
     3901 gcatcatgta gaggtactgg tcgggcgacc catcgtggat ggtgagatgg cgcagcaccc
     3961 ggcgatcacg gagtgaccat gaataaaggc cgttgctggt ggtcatccat gcggtgtcgg
     4021 tccccgtaca gaagaaaccg gtcatcagcg tcccggtatc gggtacatca cctgccaagg
     4081 gcaacgacac gccatccttc cacacaccga tgggcagcac agcgccagag gtccaccatt
     4141 gcccgatcgg gtccatgacc aggcgttcag ccagccggtc caggctgtcc ggaaaagcca
     4201 gggtcatggt cgtggtatcc cacgcggcac cacgtcgctc aacacgcaag gcacggttct
     4261 ccgcgtacag cagggcatgt ccttccacat cacgcagcag cgccaccggt ccgggaaggg
     4321 gcgcgtggac catcgggcgc ccatcgcgct gatcgatcgc gtacacatga ccgtcgagcc
     4381 gggcccagat ggtaccagag ggatcgttga agatgtgcga ccagctggtg ttcgcaccgg
     4441 aaccgggcaa cacgagcggt tccagccagt cggtacgctc gtcgaaaaga gccaggccac
     4501 cttggtggaa ggcc
//
LOCUS       DAOQOC010000084         4502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8416_length_4502_cov_4.053069, whole genome shotgun sequence.
ACCESSION   DAOQOC010000084 DAOQOC010000000
VERSION     DAOQOC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8416_length_4502_cov_4.053069"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1538
                     /locus_tag="PL106_02185"
     CDS             81..1538
                     /locus_tag="PL106_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31906.1"
                     /translation="MTWIKIRPDNLRSALAVALFGKLVLLLLLLVEHGVPVNGPWYFR
                     NGDVEGYIGPIESLLRGDGYQPDHRMPGYGAPYLLLRLLMGQGASLQMMVLLQLSASS
                     LAVVLIAHLGSLLSNDPRVFRWTFWLALASTYASLYDREIRPESFAVLALVGAAQQAV
                     LYFRTGSRTNVLLSGCALTWLIFMKPVYLPLLALLGLAIVLHKGAALRSRLLAVVVLG
                     IPWLVVDGAWCLRNAVKHGEFRPLTDGMLYPDLRVSIRHPLLRLMQAYGTSFIWWDPS
                     AEIRWFNIRQEENGQVQFPDRQPPLPDYVYTDACPRDSLRAIADDVARYNKTTDEGQR
                     EQLLAKVNADCDRCIASFRTEHPFHYHVTARLRMLKTFLLHSGTSTLMGRPMAQLDPV
                     RIIVRASYSFLYLASFLLGSLWALRSLVHRGSSPGSRLCAALVLYGITIFPFGVRMTE
                     SRYSTTIYLLALLLAILLVADTWTRIRHLRRRPTS"
     gene            1547..2527
                     /locus_tag="PL106_02190"
     CDS             1547..2527
                     /locus_tag="PL106_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA31907.1"
                     /translation="MPLLSVIIPVFNEARSLPLLFERLRTALGGIDHEVVLVNDGSHD
                     NSFEVMAQACAADPRIRAIDFRRNFGQTAAINAGIQHATGDVLVLMDSDLENDPADIP
                     LLLAKLDEGYDVVSGWRRDRWKGQFLTRKLPSLLANRLIGMISGVRLHDHGCTLKAYR
                     RDVIKDVYLYGQMHRFIPVYCSWQGGRVGEMPVRYEPRRFGKSNYGLFRTYKVLLDLL
                     VIKFLDKHMTRPIHFFGGAGFLSFGAALLSFSLALYFKLTGQKDFVQTPLPGITAMFF
                     IIGVLMILLGVLAEMLMRTYFESQGKRTYSVRRIIGTPAGGTPDNEPPRA"
     gene            2524..3084
                     /locus_tag="PL106_02195"
     CDS             2524..3084
                     /locus_tag="PL106_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA31908.1"
                     /translation="MNKLNLGSGEYLKTGFVNVDYYSVSKPDVEHDLSRFPYPFADDH
                     FDHVESDHCFEHLPDPFAVMKEVHRIAKNGATVIIRVPHFSRGFTHAEHKAGFDVTFP
                     YYFRKGFKGGYQGVEFETTAVRLRWFAQPYFKRTVLSPPVFWSARAMGAFFSFFANLS
                     PFLCSRLWCFWVGGFEEVEFRMRVKK"
     gene            3085..3834
                     /locus_tag="PL106_02200"
     CDS             3085..3834
                     /locus_tag="PL106_02200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA31909.1"
                     /translation="MTDTRFDRDQFAGAYPPGIERSWWHVARNRTIARRINANLPRGA
                     RLLEVGCGTGIVTCYLHDLGWDVTGVDLGEPRSGLRCPDRLILGQDATDLPEQQRLGV
                     DALLLFDVIEHVADAPAFLRTMLASFPNVRWVVVTVPARKELWTTFDDHFGHFRRYDR
                     IMLRDEFARSGLRTTHNAYFFHALYGAIGLNNLLRGRKRAIAFRAPARGFASMMNRSV
                     GTLMALGGMLLPGALAGSSIIAIGEREPHHH"
     gene            3844..>4502
                     /locus_tag="PL106_02205"
     CDS             3844..>4502
                     /locus_tag="PL106_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNA31910.1"
                     /translation="MCGIAGFQGSGTIDDARRMIGRIAYRGPDNRQAVLIDDTGLAHA
                     RLSIIDLSHGADQPMRDAEGELTIVFNGEIYNYKDLRASLERTGRHAFRTSGDTEVLL
                     RMYREHGTDMLSKLNGMFAFAIHDMRDGSLFLARDRMGKKPLHYLQQDNTFAFGSELK
                     AVLAHPLAPHRIDPHALDQYLSFEYVPTPRSIVAGLRKLEPGHYLLVRNGRITEHAPW
                     WT"
BASE COUNT          801 a         1448 c         1357 g          896 t
ORIGIN      
        1 gcgggatcac atcagcggga cgacggatca cctactattg tgacggaccg gcaggaggcg
       61 ggatccgctg atcgtactga atgacgtgga tcaaaataag gcctgacaac ctgcgttcgg
      121 cattggccgt agcgttgttc ggcaaactcg tactgctgct gctgttgctg gtggagcacg
      181 gggtacccgt caatggcccc tggtatttca ggaacggtga tgtagagggg tacatcgggc
      241 ccatcgaatc gcttctgcgc ggcgatggct accagccgga tcatcggatg cccggatacg
      301 gcgccccgta tctgctgctc aggctgctca tgggacaagg agcctcgctc cagatgatgg
      361 tcctgctcca gttgtccgcg agttccctgg cggtcgtgct catcgcgcac ctgggatccc
      421 tgctcagcaa cgacccgcga gtgtttcgct ggaccttttg gctggccctc gcatccacgt
      481 acgccagtct gtacgaccgc gagatccgcc ctgagtcctt cgcggtgctg gcgttggtgg
      541 gcgcggcaca acaagccgtg ctgtatttca ggaccggctc ccgaacgaac gtgctgctgt
      601 cgggctgcgc cctcacatgg ctcatcttca tgaagccggt gtacttgccg ctactggccc
      661 tcctcgggct ggcgatcgta ctgcacaagg gggccgccct gcgcagcagg ctactggccg
      721 tggtggtcct ggggatcccc tggctcgtgg ttgacggggc atggtgcctg cgcaacgcgg
      781 tcaagcacgg ggaattccgc cccctgaccg acggcatgct gtatcctgat ctgcgtgtga
      841 gcatccggca tccattgctc cggttgatgc aggcgtacgg caccagcttc atctggtggg
      901 atccctcggc cgagatccgc tggttcaata tccgccagga ggaaaatggt caggtgcagt
      961 tcccggaccg gcaaccgccc ctgccggact acgtctacac ggacgcctgt ccacgcgatt
     1021 cattgcgggc catcgcggac gatgtggcgc ggtacaacaa gaccacggat gaaggccaac
     1081 gcgaacaact gctggccaag gtgaatgccg actgcgatcg gtgcatcgcg agtttccgaa
     1141 cggaacatcc gttccattat cacgtcaccg cacggctgcg tatgctgaag accttcctgt
     1201 tgcattccgg caccagtacg ttgatgggtc ggcccatggc gcagctcgat cccgtgcgga
     1261 tcatcgtgcg ggccagttat tcgttcctct acctggcttc cttcctcctg ggatccttgt
     1321 gggcgttacg ttcgctggtc catcgcggat cctcccctgg ttcacggttg tgcgcggcgc
     1381 tcgtgttgta cgggatcacc atctttcctt tcggtgtacg catgacggag tcccgataca
     1441 gcacgaccat ctatctgctg gccttgctgc tggccatctt gctcgtcgcg gacacgtgga
     1501 cccggatccg ccatcttcgc cgtcgaccga catcctgacc ggccccatgc cccttctctc
     1561 cgttatcata cccgtattca acgaagcccg cagcctcccg ttgctctttg agcggctgcg
     1621 gaccgcgctc ggaggtatcg atcacgaagt ggtgctggtg aacgacggct cgcacgacaa
     1681 cagcttcgaa gtgatggccc aggcctgtgc ggccgacccc cggatccggg ccattgactt
     1741 ccgccgcaac ttcggacaga cggcggccat caatgcgggg atccagcatg ccacaggcga
     1801 tgtgctcgtg ctcatggaca gcgacctgga gaacgacccg gcggacatac cgctgctgct
     1861 ggccaagctc gacgaagggt acgatgtggt gagcggctgg aggcgcgacc gttggaaggg
     1921 ccagttcctc acgcgtaaac tgccttcgct gctggccaac cggctcatcg gcatgatcag
     1981 cggtgtgcgc ctccacgatc atggctgcac gttgaaggcc taccgtcgtg atgtgatcaa
     2041 agacgtctac ctgtacggcc agatgcaccg gttcatcccg gtgtattgca gctggcaggg
     2101 cgggcgggtc ggagagatgc ccgtgcgtta cgagccacgc cggttcggca agagcaacta
     2161 cggcctgttc aggacctaca aggtgctgct ggacctcctg gtgatcaagt tcctggacaa
     2221 gcacatgacc aggcccatcc atttcttcgg cggtgccggt ttcctctcct tcggtgccgc
     2281 actgctctcc ttctcgctgg ccttgtattt caagctcacc ggtcagaagg acttcgttca
     2341 gacccccttg cccgggatca cggcgatgtt cttcatcatc ggggtgctca tgatcctgct
     2401 gggcgtgctg gccgagatgc tcatgcgcac ctacttcgag agccagggca aacggaccta
     2461 cagcgtgcgc cgcatcatcg gtacacccgc gggcggaaca cctgacaacg aaccgccccg
     2521 cgcatgaaca aattgaacct cggaagcggt gaatacctga aaacaggttt cgtcaacgtc
     2581 gactactaca gcgtcagcaa acccgatgtg gagcatgacc tgagccgatt cccctacccg
     2641 ttcgccgacg accacttcga tcatgtggag agtgaccact gcttcgagca cctgcccgac
     2701 cctttcgccg tgatgaagga ggtgcatcgc atcgcgaaga acggcgccac ggtgatcatc
     2761 cgggtgcccc atttcagtcg tggcttcacc catgccgagc acaaggccgg cttcgatgtg
     2821 acctttccgt actacttccg caagggattc aaaggcggct accagggtgt ggagttcgaa
     2881 accacggcgg tgcgactgcg ttggttcgcg caaccctact tcaaacgcac ggtgctctcg
     2941 cccccggtgt tctggagcgc ccgcgccatg ggcgcattct tctcgttctt cgccaacctg
     3001 agcccattcc tgtgttcccg gctgtggtgc ttctgggtgg gagggttcga agaggtcgaa
     3061 ttccgcatgc gcgtgaaaaa gtgaatgacc gacacacgat tcgaccggga ccagttcgcc
     3121 ggggcatacc cgcccggcat cgagcgttcc tggtggcacg ttgcccgcaa ccgcaccatc
     3181 gcccggagga tcaacgcgaa cctgccacgg ggcgcacgcc tgctcgaggt aggatgcggc
     3241 accggcatcg ttacctgcta tttgcacgac ctcggctggg acgtgaccgg cgtggacctc
     3301 ggagagcccc ggtcgggtct gcgttgcccg gatcgtctga tccttggcca ggacgccacc
     3361 gatctgcccg agcagcagcg cctcggcgtt gatgcgctcc tgctcttcga tgtgatcgag
     3421 catgtggccg atgctccggc gttccttcgc accatgctcg cctcctttcc caatgtccgg
     3481 tgggtggtgg ttaccgtacc cgcccgcaaa gaactttgga ccacttttga cgaccacttc
     3541 ggccactttc gccggtatga ccggatcatg cttcgcgatg aattcgcacg cagcgggctg
     3601 cgcacgacgc acaacgccta cttcttccat gccctctacg gggcgatcgg cctgaacaac
     3661 ctgctgcgcg ggcggaagcg ggccatcgca ttccgggccc cggcgagagg tttcgccagc
     3721 atgatgaacc ggtccgtggg caccctcatg gcactgggcg ggatgctcct ccccggcgcg
     3781 ctcgcgggtt cgtcgatcat cgcgatcggt gaacgcgaac cccaccatca ctgagaaacg
     3841 accatgtgcg ggatcgccgg cttccagggg agtggtacca ttgatgacgc acggcgcatg
     3901 atcggacgca tcgcctatcg cggtccggac aaccgacaag ccgtgctgat cgacgacacc
     3961 ggactggcgc acgcccgctt gagcatcatc gatctgagcc atggtgccga tcagccgatg
     4021 cgcgatgcgg aaggcgagtt gaccatcgtg ttcaacggcg agatctacaa ctacaaggac
     4081 ctgcgcgcat cgctggagcg gacgggcagg cacgccttcc gtacgtcggg cgacaccgag
     4141 gtcctgcttc ggatgtaccg cgaacacggt acggacatgc tgtcgaagtt gaacggcatg
     4201 ttcgcattcg ccatccacga catgcgcgat ggatcgttgt tcctcgcacg cgaccgcatg
     4261 gggaagaagc cgctccatta cctgcagcaa gacaacacct tcgcgttcgg cagcgagttg
     4321 aaagccgtgc tggcacatcc gctcgcaccg caccgcatcg acccgcacgc gttggaccag
     4381 tacctgagct tcgagtacgt gccgacaccc cggagcatcg tggcgggctt gcgcaagctc
     4441 gaaccggggc attacctcct ggtgcgcaac gggcgcatca cggaacacgc cccctggtgg
     4501 ac
//
LOCUS       DAOQOC010000085         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8524_length_4465_cov_3.324490, whole genome shotgun sequence.
ACCESSION   DAOQOC010000085 DAOQOC010000000
VERSION     DAOQOC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_4465_cov_3.324490"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PL106_02210"
     CDS             <1..250
                     /locus_tag="PL106_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31911.1"
                     /translation="FFLDRGQDPKVALQYAQKSLSMEKHYWNTFTLAKAYAAVGDHAK
                     AAATAKEAAALAEADKSAGAAKEYSAKAQEWATKAAGK"
     gene            complement(306..2015)
                     /locus_tag="PL106_02215"
     CDS             complement(306..2015)
                     /locus_tag="PL106_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNA31912.1"
                     /translation="MGPLDWAVMLGTLVLIAAYGTWKTRRDGTREDYLRGGHADRWWV
                     VGFGVVATQLSAVTFISTPGQGFLDGMRFVQFYFGLPLAMIVVAAVFIPLYYKWNVYT
                     AYEFIGRRFDRRARLLTALLFLCLRGLSAGITIYAPMLVLDRVLGVGYGWSFALTGLV
                     IVYTTVGGARAVSATQTQQMAVIFLGLIVTLLLVIHQLSDRMTFSESLHLAGAAGRMN
                     IITTNFDADDKFTLWSGLIGGFFLQLSYFGTDQSQVGRYLNGRTVREAKLGMMFNGLV
                     KIPMQFIVLLTGVLVFVYFLFHPSPVHWNAQNAAALKGVPAFDSLNAGHDRHEADVRE
                     HAVAWLDALRADDADRKTSSREALNNALVADAAVRDSVRALVEERLPGSDPRDDDHIF
                     LSFVMSHFPTGILGLFVAVMFCASMSTSAAELSALASTTTVDVIRRDWSEQGQIRATR
                     WATIAFGLLAMAFAGVASQLGNLIQAVNTIGSLFYGTILGIFLVAFLLKRVQGLALFV
                     AALVAEAVILVLFALQSYEVIRPIGFLWFNLIAPAIVLLGASGLQALLPGAGIERTID
                     RSE"
     gene            complement(2128..2283)
                     /locus_tag="PL106_02220"
     CDS             complement(2128..2283)
                     /locus_tag="PL106_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31913.1"
                     /translation="MNDASQSGRDPDHGNVDRPPFLTTWRNVYLFMVVVLTIEIGIMY
                     WLTHHFA"
     gene            complement(2280..>4465)
                     /locus_tag="PL106_02225"
     CDS             complement(2280..>4465)
                     /locus_tag="PL106_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HNA31914.1"
                     /translation="QELLEARRIDGGEQFFTRAVDFGYSKNAAESFAKWGKQEVLSDV
                     VRVIRMFKPDIIITRFAPDRSAGHGHHEASAILAEEAFDRAGDPTAFPEQLTQGLQVW
                     KPRRLFFNGSTWWKKDLAVIARTDPDWYTVDVGGYDPLLGASYTEIAGRSRSMHKSQG
                     FGAAETRGEMLEYLKLIKGDKPTNGDIFQGIDMTWGKVGSSGGVQLLVDSLVSNYLPT
                     RPDQSAPLLSDLHRALAAMDKKGDPQNWLPVKQRSVEDLLFACHGLMIEALCDEENVI
                     TGSSYTFELAATNRSGTGPILILPDGAERALLVNHTFSQQITATAPKEPDSPFWLKLP
                     HNTMYTIADPGLIGLPDMPGQLTFACNVRFGDGPVVPVQVPVQHRWVDRVQGEQTRAC
                     AVVPPASIHAGRTIVLMPSDSTTVPIGVDIYADSLHMNITCARPDGWKVEPMQQPVQG
                     KKNERIGASVAVYRTSGTEKADVDLSAFWSGGRIERTQRTIAYDHIPVQHYSTPAFFR
                     AIPLSLKVDAQRIGYLQGAGDDVPKALEQMGLLVDMIDPRTSDADELRKYDAIVTGIR
                     AYNTVKEMKDLNPVLMRYVEEGGTLVCQYNTRSSDMVLPDSLFGPYPFKLTRDRVTVE
                     EAPPTFLAMDHPLLNTPNRITAADFDGWVQERGLYFCGNLDPHYTPLIAWNDPGEKPL
                     NGALITCDHGKGRYVYTGISFFRQLPAGVPGAYRLFANLISPRNR"
BASE COUNT          842 a         1405 c         1389 g          829 t
ORIGIN      
        1 gttcttcctg gacaggggcc aggatcccaa ggtggcgttg cagtatgccc agaagagcct
       61 gtccatggag aagcactact ggaacacctt cacccttgcg aaagcatatg ccgcggtagg
      121 cgatcacgcc aaagccgcag caacggccaa ggaggccgct gctctggccg aggcggacaa
      181 gagcgccggc gcggcgaagg agtacagcgc caaggcgcag gagtgggcca cgaaagctgc
      241 tggaaagtaa gtaagataaa cgatcatgaa gccccgtccc ggacctcggg acggggcttt
      301 gttcttcatt ccgaacgatc gatcgtccgc tcgatccccg ctccgggcaa cagggcctgc
      361 agcccggagg caccgagcaa cacgatggcc ggtgcgatca gattgaacca caggaagccg
      421 atcgggcgga tcacctcgta cgattgcagg gcgaagagca cgaggatgac cgcctcggcc
      481 accagtgcgg ccacgaacaa cgccaggccc tgaacgcgct tcagcaggaa ggccacgagg
      541 aagataccaa gaatggttcc gtaaaacagg gagccgatgg tgttcacggc ctggatcagg
      601 ttgcccaact gggaggccac ccccgcgaag gccatggcca gcaggccgaa tgcgatggtg
      661 gcccagcgcg tggcgcggat ctggccttgt tcgctccagt ccctgcggat cacgtcgacc
      721 gtggtggtgc tggccagggc gctcaactcg gcggcactgg tgctcatgct ggcgcagaac
      781 atcacggcca cgaacagccc gagtatgccg gtcgggaagt gggacatgac gaagctgagg
      841 aagatgtggt cgtcatcacg cggatcggag ccgggcaggc gctcctccac cagggcgcgt
      901 acgctgtcgc gtaccgcggc atcggcgacc agggcattgt tcagcgcttc acgtgaactg
      961 gtcttgcgat cggcatcatc ggcacgcaag gcatcgagcc aggccacagc gtgttcccgc
     1021 acgtccgctt cgtgccgatc gtgccccgcg ttcagcgaat caaaggcagg tacccccttc
     1081 aacgcggccg cattctgtgc gttccagtgt accgggctcg ggtggaagag gaagtacacg
     1141 aataccagca ctccggtgag caggacgatg aattgcatcg ggatcttcac caggccgttg
     1201 aacatcatgc cgagcttcgc ctcgcgaacg gtcctcccat tcaggtagcg ccccacctgg
     1261 ctctggtcgg tgccgaagta cgagagctgc aggaagaaac caccgatcag cccgctccac
     1321 aaggtgaact tgtcgtcagc gtcgaagttg gtggtgatga tgttcatccg ccccgcagca
     1381 cccgccagat gcaggctctc gctgaaggtc atgcggtcgc tcaactgatg gatgaccaac
     1441 aggagcgtga cgatgaggcc caggaagatc acggccattt gctgggtctg ggtggcactg
     1501 acggcccggg cacctcccac cgtggtgtac acgatcacca gcccggtcag ggcgaagctc
     1561 cagccgtagc ccacgcccag caccctgtcc aataccagca tcggggcata aatggtgatg
     1621 cccgcgctca gtccgcgcag gcacaggaag agcagggcgg tgagcagtct tgcacgccgg
     1681 tcgaagcgcc tgccgatgaa ttcgtacgcg gtgtacacgt tccatttgta gtagagcggg
     1741 atgaacaccg ccgccaccac gatcatggcc aggggcaggc cgaagtagaa ttgcacgaac
     1801 cgcatgccgt ccaggaagcc ctgccccggg gtgctgatga aggtgacggc actgagttgc
     1861 gtggccacca caccgaagcc caccacccac catcggtccg catggccgcc gcgcagatag
     1921 tcctcacgcg tgccgtcccg ccgggttttc catgtgccgt atgccgcgat cagcaccagg
     1981 gtgcccagca tcaccgccca atccaatggt cccatgatca gggcattgtg gccttgacgt
     2041 gcgcgatccc tggacgggtc cgcctcgtgc ccatcacgac cctgggggac agtatccggt
     2101 cctttgaggg atgaaccccg tactccctca tgcgaaatga tgtgtgagcc agtacatgat
     2161 gccgatctca atggtcagta ccacgaccat gaacaggtag acgttgcgcc aggtcgtgag
     2221 gaagggcgga cggtccacgt tcccgtggtc aggatcacgg cccgactgtg aggcgtcgtt
     2281 catcggtttc gcggggagat caggttcgcg aagagccggt aggctcccgg tacgccggcc
     2341 gggagctgcc ggaagaaact gatgcccgtg taaacatacc ggcccttccc gtggtcgcag
     2401 gtgatgaggg cgccgttgag cggtttttca cccgggtcgt tccaggcgat cagcggtgtg
     2461 tagtgcggat cgaggttccc acagaagtac aacccgcgtt cctgcaccca tccgtcgaag
     2521 tcggctgcgg tgatcctgtt cggagtgttc aacagcggat ggtccatggc gaggaacgtg
     2581 ggtggtgcct cttccaccgt tacccgatcg cgggtgagct tgaagggata cggcccgaag
     2641 agcgaatcag gaaggaccat gtcgctgctg cgcgtattgt actggcacac cagggtgccg
     2701 ccctcctcca cgtaccgcat cagcaccgga ttcaggtcct tcatctcctt cacggtgttg
     2761 tatgccctga tgcccgtgac gatggcgtcg tatttccgca actcatccgc atcactcgta
     2821 cggggatcga tcatgtcgac caggagcccc atctgctcca aggccttggg cacatcatcc
     2881 ccggcacctt gcagataccc gatccgttgc gcatccactt tcaacgaaag ggggatcgcg
     2941 cggaagaacg cgggtgtgga gtagtgctgg accgggatgt ggtcgtacgc gatggtccgt
     3001 tgggtgcgct cgatccggcc tccgctccag aaggcggaaa gatccacatc ggccttctcc
     3061 gtcccgctgg tcctgtacac cgccaccgat gcgccgatac gctcgttctt cttgccttgt
     3121 accggctgtt gcatcggttc gaccttccag ccgtccgggc gagcgcacgt gatgttcatg
     3181 tgcagggaat ccgcataaat gtccacgcct attggtacag tggtcgaatc cgacggcatg
     3241 agcacgatcg tccgcccggc atggatcgag gcgggtggca ccaccgcgca ggcccgggtt
     3301 tgctcgccct gcacccggtc cacccagcga tgctgcacgg ggacctgtac cgggaccacc
     3361 ggcccatcgc cgaatcggac attgcatgcg aaggtcagct gcccgggcat atcaggcagt
     3421 ccgatcaggc ccggatcggc gatggtgtac atggtgttat gcggcagctt caaccagaat
     3481 gggctgtccg gttctttcgg ggctgttgcc gtgatctgct ggctgaacgt gtggttgacc
     3541 agcaaggctc gttcagcgcc atcgggcagg atcaggatcg gacctgttcc ggaccggttg
     3601 gtcgcggcca gttcgaacgt gtacgacgat cctgtgatca cgttctcttc atcgcacagc
     3661 gcctcgatca tcagcccgtg gcaggcgaac agcaggtcct ccaccgatcg ctgcttcact
     3721 ggaagccagt tctgcgggtc ccctttcttg tccatggccg ccagggcacg gtggaggtcg
     3781 gagaggagtg gagcgctctg gtcggggcgg gtcggcaggt agttgctgac caaggaatcg
     3841 accaggagtt gcacgcctcc cgatgagccc accttgcccc acgtcatgtc gatcccctgg
     3901 aaaatatccc cgttcgttgg tttatccccc ttgatcaact tcagatattc cagcatctcg
     3961 ccccgtgttt ccgccgcgcc gaacccctgg ctcttgtgca tgctccggct ccgtcccgct
     4021 atctcggtat agctcgcccc gagcagggga tcgtatccgc ccacgtccac cgtgtaccaa
     4081 tccggatcgg tcctggcgat caccgcgaga tccttcttcc accatgtgct tccgttgaag
     4141 aagagccgac gcggcttcca cacctgcagt ccttgcgtca gttgttcggg gaaggccgtg
     4201 gggtcgcccg cccgatcgaa ggcctcttcg gcgagtatcg cgctggcttc atgatggccg
     4261 tggcccgccg accggtcggg ggcgaagcgg gtgatgatga tgtcgggctt gaacatgcgg
     4321 atcacgcgca ccacatcgct cagcacttcc tgcttgcccc acttcgcgaa gctctctgcc
     4381 gcgttcttgc tgtagccgaa gtccaccgcg cgggtgaaga actgctcacc gccatcgatg
     4441 cggcgtgcct ccagcagttc ctgcg
//
LOCUS       DAOQOC010000086         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8530_length_4464_cov_3.520526, whole genome shotgun sequence.
ACCESSION   DAOQOC010000086 DAOQOC010000000
VERSION     DAOQOC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_4464_cov_3.520526"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PL106_02230"
     CDS             complement(<1..1268)
                     /locus_tag="PL106_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31915.1"
                     /translation="MTGTGQGFRRTHVAALALAAIMLPWSEFLLSNAQFLLLGNWLVE
                     GIVRKDLGGRFRRAFTHPGALVFMSFFGLHALGLLWTDDMAWGLDLCRILLPVIAFTP
                     VLASVPPLSRSELRAVLLFGAWSTFASTLVCLIMRYPVLAEGGYRELSVFISHIRLAL
                     LLCFSIAVFVTHAGRGALIRSAHAVAIAWCIAFLALLHSLPGFGMLLVMAAYGIWHWS
                     ASRGTLLRRSSRLALLLIPVLVLAWVDRVRTEFHRTDATDLARLDERSAGGEYYYHDR
                     ERPLLENGHYVWINVADSELMRGWEERSVLRYHGLDARGDTLRHTLVRYLASKGLRKD
                     SLGVRALSEEDVHRVENGVPNVEAGRKGPLRARLEQILYEWDEYSVSGDASGHSVAMR
                     REFTRTGWAIAREHPVIGTGTGGTRPAFAR"
     gene            complement(1288..2952)
                     /locus_tag="PL106_02235"
     CDS             complement(1288..2952)
                     /locus_tag="PL106_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA31916.1"
                     /translation="MRITWKRTIIAGSIAAGLGFGMAAGDDYFEIGKNLEIFNELYKE
                     LNIYYVDETKPGDLMTTGIDAMLESLDPYTQYIAESEMEDYRFQTTGQYGGIGAIISK
                     KENGIAIREPYEGSPALRSGIRAGDLILEVDGRSTKGLDTEEVSKMLKGQAGTPVKIL
                     VQRNNASPELRSITREEIKIPDVPYKGMVDETGKVGYLKLNSFTQTAGTEVRNAVKEL
                     KDQGASKVILDLRGNGGGLLREAVNIVNLFTPKGETVVETKGRMADWDKTYKTLSEPL
                     DADIRLVVLTDTGSASASEIVSGALQDLDRAVVMGQRTFGKGLVQQTRDLLYNSKLKV
                     TVAKYYIPSGRCIQKLDYAQHDSTGHAVVRADSSLRGFKTRNGRPVIDGRGILPDVLV
                     EEPEMPKVVVGLFAGDVLFDFANQYYWSHDSIPPPGTFTITDDLWKDFTAFAHTRKVE
                     YRTESNEELKKLEEAAKRERYYDHAKEAFGSLRAELNPDVGQELERFRPEIQRLLLSE
                     LIGRYYHRTGQAKAMLASDPLVTRAIEVLNGAEYEGILKGTIKPGN"
     gene            complement(3293..3505)
                     /gene="yidD"
                     /locus_tag="PL106_02240"
     CDS             complement(3293..3505)
                     /gene="yidD"
                     /locus_tag="PL106_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNA31917.1"
                     /translation="MIARLLIGLVRVYQYGLSPLLPGSCRYTPSCSHYAVEALRKHGA
                     WRGGRLALKRILSCHPWGGHGHDPVP"
     gene            complement(3508..3906)
                     /locus_tag="PL106_02245"
     CDS             complement(3508..3906)
                     /locus_tag="PL106_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNA31918.1"
                     /translation="MVTPRKTFRKHERLTGRDAINALMKEGSAMNDRPLRVVGFLTPA
                     PDGAPARVAFAIPKRHVRSAVDRNRIRRRMREAYRLEKDRWYQALNNAGTRCVWLFIW
                     QSAEIPAYKGVHSKLVRLADRWLRTNLPKG"
     gene            complement(4144..>4464)
                     /locus_tag="PL106_02250"
     CDS             complement(4144..>4464)
                     /locus_tag="PL106_02250"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNA31919.1"
                     /translation="LDKSGVKYTNPIFYRTTAADLSDLKDVTYDMLVFFSPGDLESLK
                     KNFPKFKQNGTVIAAFGPTTIKAVRDAGLRLDIEAPMPEAPSMTGAIELYIKKVGKEA
                     KKKG"
BASE COUNT          782 a         1366 c         1468 g          848 t
ORIGIN      
        1 atccgcgcga atgcgggccg ggtgcccccg gtacccgtgc cgatgaccgg atgctcgcgc
       61 gcgatggccc agcccgttcg ggtgaactcc ctccgcatgg ccaccgagtg gccgctggca
      121 tccccggaaa ccgaatattc atcccattcg tacaggatct gttcgaggcg cgcacgcagc
      181 ggacctttcc gtccggcctc cacattcggc acgccgttct ccacgcggtg cacatcctcc
      241 tcggagagtg cgcgcacacc gagggagtcc ttgcgcaggc ccttgctcgc cagataccgc
      301 accagggtat gcctcagcgt atcgccccgg gcgtccagac cgtggtagcg caagacgctg
      361 cgctcctccc aaccgcgcat cagctcgctg tcggccacat tgatccatac ataatgcccg
      421 ttctccagca acggccgctc ccggtcgtgg taataatact cgcccccggc actgcgctca
      481 tccaatcgtg ccaggtcggt agcgtccgtg cggtggaact ccgtgcgcac ccggtccacc
      541 caggcgagga cgaggacagg gatcagcagc agcgccaggc gtgacgagcg tcgcaacaag
      601 gtgccgcggg aagcgctcca gtgccagatg ccgtaggcgg ccatgaccag gagcatgccg
      661 aagccgggga gactgtgcag cagggcgagg aatgcgatgc accaggcgat ggccaccgca
      721 tgggccgaac ggatcaacgc gcctcgaccg gcatgcgtca cgaataccgc gatactgaag
      781 cacaggagca aggccaggcg gatgtggctg atgaagaccg agagttcgcg gtagcctccc
      841 tcggccagga cgggataccg catgatcagg cagacgagcg tgctggcgaa ggtgctccag
      901 gcgccgaaca gcagaacggc gcgcaactcg ctccggctga gcgggggaac ggaggccagc
      961 accggtgtga acgcgatgac ggggagcagg atgcggcaga ggtcgagccc ccaggccata
     1021 tcgtcggtcc aaaggaggcc caacgcatgc aggccgaaga acgacatgaa taccaaggca
     1081 ccgggatggg tgaacgcgcg tcggaaccgg ccaccgaggt ccttgcgcac gatgccttca
     1141 accagccagt tgcccagcag gaggaactgc gcgttgctca ggaggaactc gctccacggc
     1201 agcatgatgg ccgccaaagc gagggccgca acgtgcgtcc gcctgaagcc ttggccggtc
     1261 ccggtcatgg gtggtccggc cagcaggtca gttgccgggc ttgatggtgc ctttcaggat
     1321 gccctcgtac tcggcaccgt tgagcacttc gatggcgcgt gtgacgagcg ggtcggaggc
     1381 gagcatggct ttggcctggc cggtccggtg gtaatagcgg ccgatgagtt cgctcagcaa
     1441 aaggcgctgg atctcggggc ggaatcgctc cagttcctgg ccgacgtccg ggttcagttc
     1501 ggcgcggaga gatccgaagg cctccttggc atgatcgtaa taccgttcgc gcttggcggc
     1561 ctcctcgagc ttcttcaact cctcattgct ctccgtgcgg tactccacct tgcgcgtgtg
     1621 ggcgaatgcg gtgaaatcct tccagaggtc atcggtgatg gtgaaggtgc ccggaggggg
     1681 gatgctgtcg tgcgaccagt agtattgatt ggcgaaatcg aagaggacgt caccggcgaa
     1741 cagaccgacc acgaccttcg gcatctcggg ttcctccacc agcacgtcgg gcaggatgcc
     1801 gcggccatcg atcaccggtc tgccattgcg ggtcttgaaa ccgcgcaagg atgaatcggc
     1861 acgcaccacg gcatggcccg tgctgtcgtg ctgggcgtaa tcgagcttct ggatgcagcg
     1921 accactgggg atataatact tcgccacggt gaccttgagc ttgctgttgt agagcaggtc
     1981 gcgggtctgt tgcacgaggc ccttgccgaa ggtgcgctgc cccatcacca cggcgcgatc
     2041 caggtcctgc agggcaccgc tcacgatctc gctggcgctg gcgctgcccg tgtcggtgag
     2101 caccaccagg cggatatcgg catcaagggg ctcactgagc gtcttgtacg tcttgtccca
     2161 atcggccatg cggcccttgg tctccaccac ggtctcgccc ttgggcgtga agaggttgac
     2221 gatgttcacc gcttcgcgca gcaggccacc gccgttcccg cgcaggtcga ggatcacctt
     2281 gctcgcgccc tggtccttca gttccttcac ggcgttgcgc acctcggtgc cagcggtctg
     2341 ggtaaagctg ttcagtttca ggtagcccac cttgccggtc tcatccacca tgcctttgta
     2401 gggcacgtcg gggatcttga tctcttcgcg ggtgatggac cggagttcgg gggacgcgtt
     2461 gttgcgctga acgaggatct tcacgggagt accggcctga cccttgagca tcttgctcac
     2521 ctcctcggta tcgagtccct tggtggagcg gccgtccacc tccaggatca ggtcgcccgc
     2581 gcggatgccg ctgcgcagcg ccggtgatcc ctcatacggt tcgcggatgg cgatgccgtt
     2641 ctccttcttg ctgatgatgg caccgatgcc gccgtactgt ccggtggtct ggaagcggta
     2701 gtcctccatc tcgctctcgg cgatgtactg cgtgtacggg tccaggctct ccagcatggc
     2761 atcgatgccg gtggtcatga ggtcgcccgg cttcgtctcg tccacgtagt agatattcaa
     2821 ctccttgtac agctcgttga agatctcgag gttcttcccg atctcgaagt aatcgtcgcc
     2881 agcggccatg ccgaagccga ggccggcggc gatggatccg gcaatgatgg tacgcttcca
     2941 ggtgatgcgc atgggtgtcg tgttcagttg tatgcctgcg ggtgatcgtt ggatgcgccc
     3001 gcccgttcac aaagctcggc agagcgcggg cctggggctg tgaaatttcg ccaattgacg
     3061 cgagcggtgg gcgggtgcaa gatcgcatcg atcaggccac gtccgacagg aggttgccgg
     3121 tgatacgatg ggccgacccc ctgccaagag ggagggatcg gtgggctgca cgaagctgaa
     3181 cgagggatca cggccgatcc gtacgcacgg atccgatgac atgccgtgat cgactctggc
     3241 tcatactcag cttcttgcgt gtacaagttc aacgttccgc agcgtgcgtg gatcagggta
     3301 cgggatcgtg tccgtgccct ccccacgggt ggcaggagag gatccgcttg agggcaaggc
     3361 gtcccccgcg ccaggccccg tgcttgcgga gtgcctccac ggcgtagtgg ctgcaactcg
     3421 gcgtgtagcg gcaggatccg ggcagcagag gcgagagccc gtactggtac acacgtacca
     3481 ggccgatgag gaggcgggcg atcatgccta tcccttgggc aggttcgtcc ggagccaacg
     3541 atcggcaagg cgcaccagtt tggaatgcac gcctttgtac gccggtattt cagcggactg
     3601 ccagatgaag agccagacgc atcgggtgcc cgcattgttg agtgcctggt accatcggtc
     3661 tttctccagc cggtacgcct cgcgcatgcg gcgacggatg cgattgcggt ccacagcgct
     3721 gcgcacatgg cgtttgggta tagcaaaggc cacacgggcg ggcgctccat cgggtgccgg
     3781 tgtaagaaag cccaccacgc gcagcggtcg gtcgttcatc gcggagccct ccttcatcaa
     3841 ggcgttgatg gcatcgcggc cagtgagccg ttcgtgcttg cggaaggttt tacggggggt
     3901 gaccacggcg caagatgtga taactgacgg tcttcgtaca cccggtctcc ttctgtgggt
     3961 tcaggatgac agggtgcgct gagtaggtcg agaaacatat cggcagagac tgccacgagt
     4021 ctttccggct gagcccgccg aagcctgagc ccactattac aggatgtcag gctgagcccg
     4081 tcgaagcctg agcccgccca ttgatgtcag gctgagcccg tcgaagcctg agcccgccga
     4141 agcctacccc ttcttcttgg cctccttgcc gaccttcttg atgtacagtt cgatggcacc
     4201 ggtcatgctg ggtgcctcgg gcatgggcgc ttcgatgtcc agccgcaaac cggcatcgcg
     4261 aacggccttg atggtggtgg ggccgaaggc ggcgatcacg gtcccgttct gcttgaactt
     4321 ggggaagttc ttcttcaggc tttcgaggtc gccggggctg aagaacacca gcatgtcgta
     4381 ggtgacgtcc ttcaggtcgc tgaggtcggc ggcggtggta cggtagaaga tgggattggt
     4441 gtacttcacg ccggacttgt ccag
//
LOCUS       DAOQOC010000087         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8565_length_4454_cov_3.099341, whole genome shotgun sequence.
ACCESSION   DAOQOC010000087 DAOQOC010000000
VERSION     DAOQOC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_4454_cov_3.099341"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PL106_02255"
     CDS             <1..316
                     /locus_tag="PL106_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31920.1"
                     /translation="ARTGQSAVLPGPFGTVRQPILQVLFDHERESERLNRITELTCDY
                     ALPTDADSTYVGTFQLLKEFGEDLQWHVHLEANILFPRAVEMERPAAPWQTLDDTIDE
                     LR"
     gene            313..1098
                     /locus_tag="PL106_02260"
     CDS             313..1098
                     /locus_tag="PL106_02260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA31921.1"
                     /translation="MSTLNGTDLDKAQGHWILARMGKKVLRPGGRELTEQLVGFLGIG
                     PGDDVAEFAPGLGHTAALTLGRHPRSYTGIELDPDVAAMLRGKLGGPGRTIVQGSASA
                     TGLPDACMDKVYGEAMLTMQADHRKQEIVREAHRILRPGGFYAIHELGLTPDTIDAAQ
                     KATVQKDLALSIRVNARPLSAPEWTELLEREGFVVKHIAMSPMRLLEMGRMVDDEGLW
                     RTLRISLNILRHPAARRRILDMRRVFRQHSAHMNAIALVAVKQ"
     gene            1095..1571
                     /locus_tag="PL106_02265"
     CDS             1095..1571
                     /locus_tag="PL106_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31922.1"
                     /translation="MTIVRNPIALYCTLLWVGFVCAISFLEAWLKFRAPGVTLPIGLG
                     IGRLVFGALNKVELLFAAAVVIDLLRTRTPVRQWALIVLPVVLLLLQTVWLLPVMDAR
                     AELVIQNGPAPASHMHIVYVVFEVLKVGALALFGIHSFVPTHATTPIAPMPVRSGR"
     gene            1643..2377
                     /gene="ric"
                     /locus_tag="PL106_02270"
     CDS             1643..2377
                     /gene="ric"
                     /locus_tag="PL106_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNA31923.1"
                     /translation="MNTFPSRTIGSIVADDFRAAAVFTSHGIDFCCKGGRTIQEVCSA
                     KGIDPATLENEIKATLDRDGAPADDPNSWSLTRLAEHVERVHHGYINERTPVLQQYLT
                     KLCKVHGGQHPELFEVAREFEGCAGAMATHMKKEELILFPFITRLELAKREGSAPPVP
                     GFGTVENPVRMMMHEHDEEGERFRRIAALTSGYNPPADGCNTYRAAFVLLKEFEQDLH
                     RHIHLENNILFPKAVALEAELRHATA"
     gene            2711..2857
                     /locus_tag="PL106_02275"
     CDS             2711..2857
                     /locus_tag="PL106_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31924.1"
                     /translation="MCYLHTPNGLIACKLGEKFDKGIGVVHTARNWNTVLKMHELAQG
                     LEGK"
     gene            complement(2866..3858)
                     /locus_tag="PL106_02280"
     CDS             complement(2866..3858)
                     /locus_tag="PL106_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNA31925.1"
                     /translation="MNRWTTRNAMVSAKCYGCYQALGATERLYHARCSRKLFGTPVPP
                     TLPFMEQDILGLAEQSVLRSATVAGVQTKLSLGLMRGDSSASPDRFTILGVWGSHILK
                     PPTPNYPHMVELEDLTMHLAELAGIRTVPHGLLPMEDGTLAYITRRVDRIGKPGGRKV
                     TKLHMEDLCQLTERLTEHKYRGSHEQIAKVILRFATNTLSDQTSFYEQVLFSFLTGNA
                     DMHLKNFSLLKDAEGHYNMSPGYDLVPSSLLLNETEELALTLNGRKSKLTRSDFETAM
                     RAASLNDRAILNLFNRFAKALPKWYAFIPLSFVPEELQERYIKLIDDRAERMGL"
     gene            complement(3818..4162)
                     /locus_tag="PL106_02285"
     CDS             complement(3818..4162)
                     /locus_tag="PL106_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNA31926.1"
                     /translation="MMRSAQVLMRDQWAGTLAETVDGFEFTYRPDYRANADAEAVSLT
                     LPLNERPYRSKVLFPFFDGLIPEGWLLTVAEKSWKLDPRDRMGLLLVCCKDCIGAVSI
                     EPMDNPQRDGER"
     gene            complement(4152..4394)
                     /locus_tag="PL106_02290"
     CDS             complement(4152..4394)
                     /locus_tag="PL106_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA31927.1"
                     /translation="MDAHNLADFVVQRRKALKLTQPQLADRVGVGLRFVRELEGGKPT
                     LRMDKVNEVLRFFGCELGPVPIDRSALNDNTSTNDA"
BASE COUNT          865 a         1382 c         1353 g          854 t
ORIGIN      
        1 cgcgcgcacc ggccagagcg ccgtgctgcc cgggcccttc ggcaccgtgc gccagcccat
       61 cctccaagtg ctgtttgacc atgagcggga gagcgagcgg ctcaaccgca tcaccgaact
      121 gacctgcgac tacgccctgc ccaccgatgc cgacagcacg tatgtgggta ccttccagct
      181 gctgaaggaa ttcggcgagg acctgcagtg gcacgtgcat ctggaagcga acatcctctt
      241 cccccgtgcg gtggagatgg aacgcccggc cgcaccttgg caaacattgg atgatacgat
      301 cgatgaattg agatgagcac attgaacggg accgacctgg acaaggcgca aggacattgg
      361 atcctggcgc gcatgggcaa aaaagtactg cgcccgggtg ggcgcgaact caccgaacag
      421 ctcgtgggct tcctcggtat cggacccggg gacgatgtgg ccgagttcgc gcccggcctc
      481 ggccataccg cagcgctcac attgggacgc cacccgcggt cgtacaccgg catcgagctc
      541 gaccccgatg tcgcggccat gttgcgcggg aagctcggag gacctggtcg taccatcgta
      601 cagggatcgg catccgccac cggattgccc gacgcgtgca tggacaaagt gtacggggag
      661 gccatgctca ccatgcaggc cgatcaccgc aagcaggaga tcgtgcgcga agcccatcgg
      721 atcctccggc cgggcgggtt ctacgccatc catgagctcg gtctcacccc cgataccatc
      781 gatgcggcgc agaaggcgac cgtgcagaag gaccttgccc tgtccatccg ggtgaacgca
      841 cgtccactca gcgcacccga atggaccgaa ctgttggagc gcgaaggatt cgtggtgaag
      901 cacatcgcca tgagcccgat gcggcttctg gagatgggcc gtatggtgga tgatgaaggt
      961 ctttggcgca cgctgcgcat cagcctgaac atcctgcgcc atccggctgc ccgtcggcgc
     1021 atcctggata tgcgtcgcgt cttccggcaa cacagtgccc acatgaacgc catcgccctg
     1081 gtggccgtga aacaatgacc atcgtccgca acccgatcgc cctttactgc acgctgctgt
     1141 gggtgggctt cgtgtgcgcg atcagtttcc tggaggcctg gctgaagttc cgtgcgccgg
     1201 gggtcacact gcccatcggc ctgggcatcg gtcggctggt gttcggtgcg ctcaacaagg
     1261 tggaactcct gttcgccgcc gctgtggtga tcgatctgtt gcgcacacga acacctgtac
     1321 ggcaatgggc cttgatcgtg ctacccgtcg tactcctgct gcttcaaacc gtgtggctgc
     1381 tgccggtcat ggacgcccgt gccgaactcg ttatccagaa cggtcccgcg ccagcatccc
     1441 acatgcacat cgtgtacgtg gtgttcgaag tcctgaaggt cggagccctc gcacttttcg
     1501 ggatccattc gttcgttcca acgcatgcca ccacgccgat cgcacccatg cctgtacgat
     1561 ccggacgatg agcgggtctt gagaacacgc ggcccttcat ccacgaccac ccaccacccc
     1621 aacgcacaca acaataacaa tcatgaacac cttcccctcc cgcacgatcg gttccatcgt
     1681 ggccgatgac ttccgcgcag cggccgtatt cacgtcccac ggcatcgact tctgctgcaa
     1741 gggtggccgt accatccagg aagtatgcag tgccaagggt atcgaccccg ccacgttgga
     1801 gaacgagatc aaagcgacac tggaccgcga cggcgcgcct gcggatgacc ccaactcctg
     1861 gtcgctcacc cgcctggccg agcacgtgga gcgcgttcat cacggataca tcaacgagcg
     1921 caccccggtg ctgcaacagt acctcaccaa gctgtgcaag gtgcatggtg ggcaacatcc
     1981 ggaactcttc gaggtcgctc gcgagtttga gggttgtgcc ggagccatgg ccacgcacat
     2041 gaagaaggag gaattgatcc tcttcccttt catcacccgc cttgaactgg ccaagcgcga
     2101 ggggagcgca ccgcccgtgc ccggcttcgg caccgtggag aaccccgtgc gcatgatgat
     2161 gcacgagcac gatgaagaag gtgaacgttt ccggcggatc gccgcactga ccagcggata
     2221 caacccgcct gccgatggtt gcaacaccta ccgcgccgcg ttcgtcctgc tgaaggagtt
     2281 cgagcaggac ctgcatcggc acatccatct ggagaacaat atcctcttcc cgaaggcggt
     2341 ggccctggaa gccgagttgc ggcatgctac cgcgtgatcg gtatgggtgg tgaggtccgg
     2401 ggaggagaat ggacctctcc ggttgaatag tccggtccat gtggtgccgt gaggcgggtc
     2461 ctgttggtct tcccctgtct cgcctgttcc agcttgcagt accgggataa cgggcaccgg
     2521 ggtccgggca tttcagcgac cagtccatca gatcccctcc acaacctcat ttcaccctgc
     2581 cgctccgatg tccgcgtggc ccgcttgttc gatggtccga gtgcggaagc cgtgaagctc
     2641 aggcccattc gtgccgtgcg cgatgaggcg ttgaaggaga tggtcaccca gccaattcgg
     2701 acgccaggtg gtgtgctacc tgcacacacc gaacggcctc atcgcgtgca agctcggcga
     2761 gaagttcgac aagggcatcg gcgtggtaca caccgcacgg aactggaaca cggtgctgaa
     2821 gatgcacgaa ctggcgcagg ggttggaggg gaagtagcgc cacggtcaca agcccatccg
     2881 ctccgcgcga tcgtcaatca gcttgatgta ccgctcctgc agttcctccg gcacgaagct
     2941 gagcggaatg aacgcatacc acttcggcag cgccttcgcg aagcggttga agaggttgag
     3001 gatggcgcga tcgttcaagc ttgcggcacg catggccgtc tcgaagtctg aacgagtgag
     3061 tttgctcttc cggccgttga gggtaagggc gagctcctcc gtctcgttga gcagcagcga
     3121 ggacggcacc aggtcgtaac ctgggctcat gttgtagtgg ccttcggcat ctttcagcag
     3181 cgagaaattc ttcaaatgca tatcggcgtt gccggtgagg aagctgaaaa gtacctgctc
     3241 gtagaaggag gtctgatcgg agagcgtgtt ggtggcgaag cgcaggatga ccttggcgat
     3301 ctgctcgtgc gagcccctgt acttgtgctc ggtcaggcgc tcggtgagct ggcataggtc
     3361 ctccatgtgc agcttggtca ccttgcggcc accgggcttg ccaatgcgat ccacacgacg
     3421 ggtgatgtac gcgagcgttc catcttccat cggcaataga ccatgcggca cggtgcggat
     3481 gccggccagc tcagcgaggt gcatggtgag gtcctccaac tccaccatgt gcggatagtt
     3541 gggcgtgggc ggcttcagga tgtggctgcc ccagacacca agtatcgtga agcggtctgg
     3601 cgatgcggaa gagtcgccgc gcatgaggcc gagcgacaac ttcgtttgca caccggccac
     3661 cgttgcgctt cgcaacacgc tctgttcagc aaggcccagg atgtcctgtt ccatgaaggg
     3721 caatgtcggc ggtaccggcg tgccgaagag cttgcggctg caccgcgcat ggtagagccg
     3781 ttccgtggcg ccgagcgctt ggtagcaacc gtagcactta gcgctcacca tcgcgttgcg
     3841 ggttgtccat cggttcaatg ctcactgcgc cgatgcagtc cttgcagcac acgagcaaga
     3901 ggcccatgcg atcgcgcgga tcaagcttcc agctcttctc ggcgacagtg agcaaccagc
     3961 cttcagggat caatccatcg aagaagggga agagcacttt gctcctgtat ggccgttcat
     4021 tcaatggcaa cgtcaggctc acggcttccg catcggcatt cgcgcgatag tcgggccggt
     4081 atgtgaattc gaacccatcc acggtctcgg cgagggtgcc tgcccactga tcgcgcatca
     4141 acacttgggc gctacgcatc attagtggag gtgttgtcgt tcagggcaga acgatcgata
     4201 ggcacaggcc ccagttcaca gccgaagaag cgcaacactt cgttcacctt gtccatgcgc
     4261 agggtgggtt tgccgccctc caactcacgc acaaagcgca gccctacacc gaccctgtcg
     4321 gccagttgcg gttgggtaag cttcaatgcc tttcgccgct gaacgacgaa gtctgctagg
     4381 ttatgagcat ccattctgta ccctttcggg gccaaattag gccaattcat tcatttcata
     4441 cctgatgggg tatg
//
LOCUS       DAOQOC010000088         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8589_length_4447_cov_4.205601, whole genome shotgun sequence.
ACCESSION   DAOQOC010000088 DAOQOC010000000
VERSION     DAOQOC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8589_length_4447_cov_4.205601"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PL106_02295"
     CDS             complement(<1..478)
                     /locus_tag="PL106_02295"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNA31928.1"
                     /translation="MKVIDHLNKAKGTLLSFEILPPLKGKDIRSIYEGIDPLLEFKPS
                     FINVTYHREEVIYKERGHGLLQKIRLRKRPGTVGICAMIKAKYDIDTVPHLICGGFSR
                     EDTEDALIELNFLGIDNVLALRGDATKGEAQFIPERDGHAHAEDLIRQIAGLNKGQY"
     gene            complement(572..2758)
                     /locus_tag="PL106_02300"
     CDS             complement(572..2758)
                     /locus_tag="PL106_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA31929.1"
                     /translation="MKLNTWISTIGGALLLTCMVLPWTTASGQGVNLSVDEYQRLKAD
                     GNLPAEFHVAYSTLPPAAVKAGVGKKPAKGGQPKGGGGEVNGDCDCWIAPDDTYTLAM
                     SPNDDQSSSQITLPFTFNLYGDLSNTCYINNNGNISFGAPYFTYSAEGFPSTNYVMVA
                     AFWADVDTRGSGQVLYKITPTALYVNWVDVGYYSMQTDKLNNFQLIITDGTDPVIGVG
                     NNVAFCYKDMQWTTGAASCGGGGTCTYNGQTYTCGGTGGFCGYPSTVGANRGNGTDFI
                     QFTRNDHPGTDYDGPFGSADGVDWLDFKSFRFTTAVSTQNIPPIVNSNFLCDTVEVCM
                     GELVNFDVTFLTPEDDQQIINTTADAPSIDNFASTVLNNGTNATINVQFIPQIADTGY
                     HVVTFTGQDNGQDSLTSTVSIVLAVYYTPAPPPVIAGDTLSCEGQGVVLSAGSGYENY
                     VWSNGYNGPVVLVGEGTYMCIATSGNCRLASNSITVYEVPNPDPVITGQLFSCGGTPA
                     TLGTTEQYAQYAWSNGEAGSNITVGTGSYFVTVTDDNGCAANSDTVNVLSANDPVAII
                     TGCTSTVFPPSEVTFYSASTIDGGTIVDADWTINDTVPVAGSDSIMYEFTAPGTYAVT
                     LAVTTADGCTDTVTCHQLVLPLEIEVPNVFSPNGDGKNDVLEFTGVEYFPGNHLRVFN
                     RWGQPIYEASSYKNTWRASGVPEGTYFYVLKLPNGKEYTGHVTLLR"
     gene            complement(2755..>4447)
                     /locus_tag="PL106_02305"
     CDS             complement(2755..>4447)
                     /locus_tag="PL106_02305"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA31930.1"
                     /translation="LIGGVPVTGGVWTDPLGNPHSGQFLPGEDPDGVYLYAVNEGGNC
                     PNSTTVTMTSAQPVNAGNDVVDQGYCASDAPVDLFTLLTGGAQTGGNWYYLNGSAFTG
                     TLHPDTDPGGDYIYVVPGTAPCSNDTAVVSLHIEQTVDAGEDASLTMCTDAAPVALTS
                     ALGGTPDPNGMWTAPGNQSSNGTFDPGTGPAGIYTYTVNAVQPCPSQSSTITIAVDQP
                     PYAGSSAVLVACDGDAAQDLFQLLGGSPEAGGTWTSANGSSHPAILDPAVDSSGTYMY
                     VVNGTAFCAHLTDTAIVVVTVNPLPIITFTVDPDSGCVPLTAAFTNTTDPVYLGGNCT
                     WDFGDGSTFAGCDPGTHEYTVPNWYNVRLEVTTPQGCTDRFTLQGAVLADPVPTAAFE
                     WTPDTGTPENSTLVFTAQDPHASVFEWSFHDGSEAGTRQAAHSYPDFQSGEYEVCLHV
                     ADRYGCADSLCETIEVRVPLVFMPNAFSPDGNGVNETFLPVVSGMVAEEHELMIFDRW
                     GQRVFQSTDPLVGWNGSMDNGGQVLPNGVYNWRLIERPIGSADKKDWFGTVTLLR"
BASE COUNT          796 a         1375 c         1385 g          891 t
ORIGIN      
        1 gatactggcc cttgttgagt ccggcgatct ggcggatcag gtcctccgcg tgggcatggc
       61 catcacgctc agggatgaac tgtgcctcgc ccttggtggc gtcaccgcgc agggccagca
      121 cgttgtcgat ccccaggaag ttgagctcga tcagcgcatc ctcggtgtcc tcccggctga
      181 agcccccaca gatcaggtgc ggcaccgtgt cgatgtcgta cttggccttg atcatggcgc
      241 agatgcccac ggtgcccggc cgcttgcgca agcggatctt ctgcaacagt ccgtggccac
      301 gttccttgta gatgacctcc tcgcggtggt aggtcacgtt gatgaagctc ggcttgaact
      361 ccagcaacgg gtcgatgcct tcgtagatcg agcggatgtc cttgcccttc aacggcggca
      421 ggatctcgaa ggacaacaat gtgcccttgg ccttgttcag gtggtcgata accttcatgg
      481 ggggcggcga agatatccgg caccggggcc tcgaaagacc gggccccgtt gcgctggtca
      541 acggggcccg gtggatgaag cgcgcgtgga ctcagcgcag cagggtcacg tggccggtgt
      601 actccttgcc attgggcagc ttcagcacat agaagtatgt gccttcagga acaccgctgg
      661 cgcgccaggt gttcttgtag ctgctggcct cgtagatggg ttgcccccac cggttgaaga
      721 cgcggagatg gttgcccggg aaatactcca ccccggtgaa ctccagcaca tcattcttgc
      781 catcgccgtt ggggctgaag acgtttggta cttcgatctc gaggggcagc accaactgat
      841 ggcaggtcac ggtatccgta catccgtcgg cggtggtcac ggcgagggtt acggcgtatg
      901 tccccggcgc agtgaactcg tacatgatcg agtcggagcc ggccaccggt acggtatcgt
      961 tgatggtcca gtccgcatcc acgatggtgc cgccatcaat ggtgctcgcg ctgtagaacg
     1021 tcacctccga aggcgggaac accgtgctgg tgcagcccgt gatgatggcc acggggtcgt
     1081 tggcgctcaa tacgttcacc gtgtccgagt tcgccgcgca cccgttgtca tcggtaacgg
     1141 tcacgaaata cgagccggtc cccaccgtga tgttcgaacc cgcttcaccg ttggaccacg
     1201 cgtactgcgc gtactgctcg gtggtcccca aggtggccgg ggtgcccccg caactgaaca
     1261 actgcccggt gatcaccggg tcggggttgg gtacctcgta caccgtgatc gaattggagg
     1321 ccaggcgaca gttgccggag gtggcgatgc acatgtaggt gccctcgccc acgagcacca
     1381 cagggccgtt gtatccgttg ctccacacat agttctcgta gccagaacct gccgaaagca
     1441 cgaccccctg gccctcgcac gacagggtgt cgcccgcgat caccggcggc ggagccggcg
     1501 tgtagtacac cgccagcacg atggacacgg tgctcgtcaa tgagtcctgg ccgttgtcct
     1561 gcccggtgaa ggtgaccacg tggtaacccg tgtcggcgat ctgcgggatg aactgcacgt
     1621 tgatcgtggc gttcgttccg ttgttcaata ccgtgctggc gaagttgtcg atcgagggtg
     1681 cgtccgccgt ggtgttgatg atctgctgat cgtcttcggg cgtgaggaag gtgacgtcga
     1741 agttcaccag ttcgcccatg catacctcca ccgtgtcgca caggaagttc gagttcacaa
     1801 tgggcggtat gttctgggtg ctcaccgccg tggtgaaccg gaagctcttg aagtcgagcc
     1861 aatccacccc atcggcgctg ccgaacggac cgtcgtaatc ggtgcccggg tgatcgttgc
     1921 gggtgaactg gatgaagtcc gttccattgc ccctgttggc ccccacggtg ctggggtagc
     1981 cacagaagcc gcccgtacca ccacaagtgt aggtctgacc gttgtatgtg catgtgccgc
     2041 caccgccgca cgaagcggcg cccgtggtcc actgcatgtc cttgtaacag aaggccacgt
     2101 tgttgcccac cccgatcacc gggtcggtgc catcggtgat gatcagttga aagttgttca
     2161 gcttgtcggt ctgcatgctg tagtagccca catccaccca attcacatac aatgccgtgg
     2221 gcgtgatctt gtacagcacc tgacccgagc cgcgcgtatc cacatcagcc cagaaggccg
     2281 ccaccatcac gtaattcgtt gatgggaagc cttcggccga gtaggtgaaa tagggcgcac
     2341 caaaggagat gttgccgttg ttgttgatgt agcaggtatt gctcaggtcg ccatacaggt
     2401 tgaaggtgaa cggcagggtg atctgcgagg agctttgatc gtcgttgggc gacatcgcca
     2461 gggtgtaggt atcgtccggc gcgatccaac agtcgcagtc gccgttcacc tcgccaccgc
     2521 cacccttggg ctggccaccc ttggcgggtt tctttccaac gccagctttc accgcagccg
     2581 ggggcagggt ggagtatgcc acgtggaatt cggccggcag gttgccatcc gccttcagac
     2641 gctgatactc atccaccgac aagttcacgc cttgtccgct cgcggtggtc caagggagca
     2701 ccatgcacgt caggagaagc gctcccccga tcgtagagat ccatgtgttc agtttcatcg
     2761 cagcagggta accgttccga accagtcctt tttgtccgcc gagccgatcg gccgttcgat
     2821 caagcgccaa ttgtacacac cattgggcag cacctggcct ccgttgtcca tcgatccgtt
     2881 ccaacccacc agcggatcgg tgctctggaa gacccgttgc ccccagcggt cgaagatcat
     2941 caattcatgc tcttccgcaa ccatcccgct caccacgggc aggaatgttt cgttcactcc
     3001 gttgccgtcc gggctgaagg cgttgggcat gaacaccagt ggcacccgca cctcgatggt
     3061 ttcgcagagc gaatcggcac agccgtagcg gtccgccacg tgcagacaca cttcgtactc
     3121 tccgctctgg aagtcagggt agctatgagc ggcctgccgc gtgccggcct cggacccatc
     3181 gtggaaactc cattcgaata ccgaggcgtg cgggtcctgt gcggtgaata ccagggtgct
     3241 gttctccggc gtgcccgtgt caggcgtcca ttcgaacgcg gccgtgggca cgggatcggc
     3301 cagcacggcg ccttgcaggg tgaaccggtc cgtacagccc tgtggcgtgg tcacttccag
     3361 gcgcacgttg taccagttcg gcaccgtgta ctcatgcgtt ccaggatcgc aaccggcgaa
     3421 ggtgctgccg tcgccgaagt cccaggtgca attgccacca aggtatacgg ggtccgtggt
     3481 gttggtgaaa gcggcggtga gcgggacgca gccgctgtcc gggtccacgg tgaaggtgat
     3541 gatcggcaag gggttcaccg taacgaccac gatcgccgta tcggtcaggt gcgcacaaaa
     3601 ggctgttccg ttcaccacgt acatataggt tcccgaactg tccaccgctg gatccaaaat
     3661 ggccggatgc gaagagccat tcgcactcgt ccatgtaccg cccgcctccg ggcttccgcc
     3721 gagcaactgg aatagatcct gtgccgcgtc cccatcgcag gcgacgagta cagcactgga
     3781 gcccgcgtac gggggctgat ccaccgcgat ggtgatcgta ctgctctgcg atggacaagg
     3841 ctgcacagcg ttcaccgtgt acgtgtatat gcccgccggg cccgtacccg ggtcgaaggt
     3901 gccgttggaa ctctggttgc cgggcgcggt ccacatgccg ttgggatccg gggtaccgcc
     3961 cagtgccgag gtcagcgcga ccggggcggc gtccgtacac atcgtgaggg aagcatcctc
     4021 ccccgcatcc acagtttgtt caatatgcaa gctgacaacg gccgtgtcat tggaacaggg
     4081 cgctgtacca ggtaccacgt agatatagtc gcccccgggg tcggtgtccg ggtgcagggt
     4141 gcccgtgaag gcgctaccgt tgagatagta ccagttacca ccggtctgcg cgcctcccgt
     4201 caacaacgtg aagaggtcca ccggcgcgtc gctcgcacag tacccctggt ccaccacgtc
     4261 gttgcccgcg ttcaccggct gcgccgaggt catggtaacg gtggtggaat tggggcaatt
     4321 gccgccctcg ttcaccgcgt acaggtacac cccgtcgggg tcctcgccgg ggaggaactg
     4381 tcccgaatgc gggttgccga gcggatcggt ccagaccccg ccggtcacgg gcacacctcc
     4441 gatcaac
//
LOCUS       DAOQOC010000089         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8649_length_4431_cov_3.345521, whole genome shotgun sequence.
ACCESSION   DAOQOC010000089 DAOQOC010000000
VERSION     DAOQOC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8649_length_4431_cov_3.345521"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PL106_02310"
     CDS             complement(<1..664)
                     /locus_tag="PL106_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA31931.1"
                     /translation="MHVLVLPKWYPGRNDPQLGDFVRKQMLATSAVLNGRNDRLSVVF
                     AVPIPGLEQLGIQELKTGDGAWELHCYYRPSSSRLLPVRKLVNLWRYWKAARNGIRRV
                     IRERGNPDLVHVHILVRPALLAWWFQQWHGTPFILSEQSSEYLDGTWDGKSAWFKAIN
                     YFLFRRAAAITAVSTHLGDGMRQRGLCSTFDVVPNTVPGTDRPLPPAGPSTSFMMVAD
                     LVD"
     gene            738..813
                     /locus_tag="PL106_02315"
     tRNA            738..813
                     /locus_tag="PL106_02315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:773..775,aa:His,seq:gtg)
     gene            complement(1092..2084)
                     /locus_tag="PL106_02320"
     CDS             complement(1092..2084)
                     /locus_tag="PL106_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNA31932.1"
                     /translation="MTGPRGSIRLQAWVLFIGMAIMAIKFLAWRLTGSDTILSDALES
                     ILNVTAGAFALYSLWLTARPRDRGHPYGHGKVEFISAGIEGGLVMLTGVGIIWRAVGS
                     LLAQQGPHHLETGILLTGSAGAINLIMGLVLRRRGRAVHSITMEASGTHLLSDAWSTV
                     AMVFGLLLIRITDIHWLDQLFAMMFAIYIIYTGIRVFRRSVAGIMDESDPALESEVIA
                     ALEVVRQPRWIDLHNFRTIRYGPALHIDCHVTLPWYLSLEEAHAEIVSMGEVVRASCS
                     RDVELFMHMDPCVPGSCPICILTECPKRRSPFIKRVEWTLDSVTADSKHSAEQT"
     gene            complement(2081..3412)
                     /gene="nhaA"
                     /locus_tag="PL106_02325"
     CDS             complement(2081..3412)
                     /gene="nhaA"
                     /locus_tag="PL106_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNA31933.1"
                     /translation="MYRRTRIGRLRQPFNALLGAQATGGVLLFMAVVVAMFWANSPWG
                     TGYHHLWENRIVLGSDQWRFDMSLHHWINDGLMAIFFFVVGLELKREMLAGELSRPQD
                     AMLPIAAALGGMVVPALIYWLWNPSGPTSTGWGIPMATDIAFALGLMAVLGRRVPVSL
                     KVFLTTLAVADDMGAVAVIAIFYPSSAISFAHLGLAALVLLVMVAGNKLGVRSMWFYA
                     LLGIGGVWTAFLASGVHATIAGVLAAFTIPAIPAIREDVFERLMRIRLFSFARHDIPD
                     KGIRSTEQLLDLEGMNRLTDHAATPLQKLEHTLHPVVTFFVLPLFALANAGVELPGDM
                     GTAFGSSIALGVTFGLLLGKPIGVLLTSFLLVRFTKARLGNGITWQHMIGAGILAGIG
                     FTMSLFVNELAFNDPLLRQEAKLGVLGGSLLAGIIGYLVLVRAPQKAVDRT"
     gene            3692..>4431
                     /locus_tag="PL106_02330"
     CDS             3692..>4431
                     /locus_tag="PL106_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNA31934.1"
                     /translation="MDVRLIEAASEIGAGKRGASMGMSALRVAAWKNGSDLFGHAEES
                     ILRDENDVLYEDDSSPNAHHIDGLIRFESDLAYEVYKFLRSNIFPMVIGGDHSIAIGS
                     ISGTKMAFPHERLGVVWIDAHADLHSPWTTPSGNVHGMPLALLMNIEKKGRNKPRVFT
                     LDTWDRLRKIGMSGPKLLPSDLVFIGLRDYEPEEAAIIREHGIKVITVDELRQRGADQ
                     VVAETLAHLTACERIHVSFDVDSLDPSI"
BASE COUNT          899 a         1349 c         1387 g          796 t
ORIGIN      
        1 gatccaccag gtcggccacc atcatgaagc tggtggatgg cccggcaggt ggcaatggcc
       61 gatcggtgcc cggcacggtg ttgggcacca cgtcgaatgt tgaacagagg ccgcgttggc
      121 gcatgccatc gcccaagtgc gtgctcacgg cggtgatcgc ggccgcacgc cggaacagga
      181 agtagtttat cgccttgaac catgcgctct tgccgtccca ggtcccgtcc aggtattcgc
      241 tgctctgctc gctcaggatg aagggtgtgc cgtgccattg ctggaaccac caggccagca
      301 acgccgggcg tacgaggatg tgaacatgca caaggtccgg gttgccccgt tcgcggatca
      361 cccttcggat cccgttgcgc gcagccttcc agtatcgcca caggttcacc agcttgcgca
      421 cggggaggag gcggctgctg ctcggtcggt aataacagtg cagttcccat gctccgtcgc
      481 ctgttttcag ctcctgtatc ccgagttgtt ccagaccagg aataggcacc gcgaaaacga
      541 cgctcagccg gtcattccgc ccgttgagga cagcggaggt agcgagcatc tgcttgcgaa
      601 cgaagtcacc caattgcggg tcgttccggc ccgggtacca tttgggaaga accagcacat
      661 gcatccaacg gcaaagtaac gtgcatgcac gtaccggcta tatttgcggc ccgatccacc
      721 cgttggtgaa cggaaatggt ggttgtagct cagttggtta gagcgtcgga ttgtggttcc
      781 gaaggtcgcc ggttcgagcc cggtctacca cccgcgacat aaaagaggcc cgcacgctgc
      841 gggcttcttt acttccagtg cgcggcgatc ccaccatacc ggcccggtat tgcccgttcc
      901 gcaggcactt cccacatgcg ggatgaagca ttttcagcag gtcatctcca gttgtccctt
      961 cgaccagggt acatgccgac ctgaccgtcc cgcaggtccg cggggcgccc ggcgaaaagc
     1021 agccgtcgat cttcaggtgc attatgaaat gcaaatcggt cggaagtgtt gcggtggaac
     1081 agccgcaaca ttcaggtctg ctccgcgcta tgcttgctat cggccgtaac gctgtccagg
     1141 gtccattcca cgcgcttgat gaacggcgag cgtcgcttgg ggcattcggt cagtatgcag
     1201 atcgggcacg atccaggaac gcaggggtcc atgtgcatga acagttccac gtcgcgtgaa
     1261 caactcgccc gaacgacctc gcccatggag acgatctcgg cgtgggcctc ctccaagctg
     1321 aggtaccatg gcaaggtcac gtggcaatcg atgtgcaacg caggtccata acggatcgta
     1381 cggaagttgt gcaggtcgat ccaccggggt tgtcgcacca cctcgagagc ggcgatcacc
     1441 tcactttcca atgcggggtc ggactcgtcc atgatgccgg ccaccgatcg gcggaagacc
     1501 cggatccccg tgtagatgat gtagatggcg aacatcatgg cgaagagttg gtccagccag
     1561 tggatgtccg tgatacggat gagcagcagg ccgaatacca tggccacggt actccatgca
     1621 tcgctcaaca ggtgggtgcc actggcctcc atggtgatgg agtgtaccgc acggccccga
     1681 cggcgcaaga ccaggcccat gatcaagttg atcgcgcccg ccgagccggt gagcaggatg
     1741 cccgtttcca gatggtgcgg accttgctgg gccagcagcg atccgacggc acgccagatg
     1801 atgcccacac cggtcagcat caccagcccg ccctcgatgc ccgcactgat gaattcgacc
     1861 ttgccatggc cgtacggatg gccccggtca cgtggccgcg cggtgagcca caggctgtac
     1921 agggcgaatg cgccggccgt cacgttcaag atgctctcca gcgcatcgct caggatcgta
     1981 tcgctgccgg tgagcctcca cgcaaggaat ttgatggcca tgatggccat gcctatgaac
     2041 agcacccagg cttgcaatcg gatggaaccg cgtgggccgg tcatgttcgg tcaacggctt
     2101 tttggggtgc acgaacaagc acgagatatc cgatgatccc ggccagcagg gatccaccca
     2161 aaacccccag cttggcttcc tggcggagca gcgggtcgtt gaaggccagt tcgttcacga
     2221 acagggacat cgtgaagccg atgcccgcca ggatgcctgc accgatcatg tgttgccagg
     2281 tgatcccgtt gcccaaccgg gccttggtga aacgcaccag gaggaaggat gtcagcaaca
     2341 cgccgatcgg tttgcccagc agaaggccga acgttacgcc cagcgcgatg gaagagccga
     2401 acgccgtgcc catgtcgccc ggcagttcga cccccgcatt ggccagcgcg aacaggggca
     2461 gaacgaagaa ggtgaccacg gggtgcaacg tgtgttccag cttctggagt ggtgtggcgg
     2521 catggtccgt caggcggttc atgccctcca ggtcaagcaa ttgttccgtg gagcggatcc
     2581 ccttgtccgg gatgtcgtgc cgcgcgaagc tgaacagacg tatgcgcatg agccgttcga
     2641 acacatcctc ccggatggcg ggtatggccg gaatggtgaa ggcggccagg accccggcga
     2701 tggtggcatg caccccgctg gccaggaagg cggtccacac cccgccaatg ccgaggagcg
     2761 cgtagaacca catgctgcgc acgcccaact tgttacccgc caccatgacg agcaggacca
     2821 gggccgccag gcccagatga gcgaacgaaa tggccgaaga cggatagaag atcgcgatca
     2881 cggctacggc gcccatgtca tcagccaccg ccagggtggt caggaagacc ttgagcgaca
     2941 cgggcacgcg cctgcccagc acggccatga gacccaaggc gaatgcgatg tccgtggcca
     3001 tgggaatgcc ccagcccgta ctggtcgggc ccgatgggtt ccataaccag tagatgagcg
     3061 caggtacgac cataccaccc agtgcggctg cgatgggaag catcgcgtcc tgcggccggg
     3121 agagctcacc ggccagcatc tcgcgtttga gctccagacc taccacgaag aaaaagatgg
     3181 ccatgagacc atcgttgatc caatggtgca gggacatgtc gaagcgccat tggtcggatc
     3241 ccaacacgat ccggttctcc cacaggtggt ggtaaccggt gccccatggc gagttggccc
     3301 agaacatcgc caccaccacg gccatgaata gcaatacacc tccggtggcc tgcgcgccga
     3361 gcagggcatt gaagggctgg cgcaagcggc cgatccgggt gcggcggtac atggtgttgg
     3421 ccggtatcag gtgcatggtg accgggccgc aaagctatcc tgcgggtccg tgtccggtcg
     3481 gatgcctgga cccgggaggg accgggtgac ggcggaaccg cgccggctgc acatggtgcc
     3541 gcctatggct tgccgtccct tcaagcagtc gccaccgaga ggtcgcaatc aaccgcccca
     3601 atggtcgaat gcgcctcgtc tcctggtcaa ctgtccttgg cgcccctgag gtcccccggt
     3661 taccttcgca gcgttccaac cccgtattcc aatggatgtt cgattgatcg aggcggccag
     3721 cgagatcggc gccggaaaac gaggtgccag catgggcatg agcgctttgc gggtggccgc
     3781 ttggaagaac ggcagcgacc tgttcggcca tgccgaggag agcatccttc gcgacgagaa
     3841 tgatgtgctg tacgaggacg acagctcgcc gaacgctcat cacatcgatg ggcttatccg
     3901 ctttgaatca gacttggcgt atgaggtgta caagttcctg cgtagcaaca tattccctat
     3961 ggttatcgga ggcgatcatt caatagcgat cggctcgatc agcggtacca aaatggcctt
     4021 tccgcatgag cgcctcggag tggtctggat cgatgcgcat gcggatctgc acagcccatg
     4081 gaccaccccc agtggcaatg tgcatggcat gccgctcgcg ttgttgatga acatcgagaa
     4141 gaaaggccgc aacaaaccgc gggttttcac cctggataca tgggaccgct tgcggaagat
     4201 cggcatgagc ggccccaagc tcctgccttc cgacctggtg ttcatcggct tacgcgacta
     4261 cgaacccgag gaggcggcga tcatcaggga gcacgggatc aaagtcataa ctgtggacga
     4321 gctgcgccag cgcggtgctg accaggtggt ggcggagacc ttggcccacc tcacggcatg
     4381 cgaacggatc catgtgagct ttgacgtgga cagcctggat ccctcgatca g
//
LOCUS       DAOQOC010000090         4331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8971_length_4331_cov_3.679607, whole genome shotgun sequence.
ACCESSION   DAOQOC010000090 DAOQOC010000000
VERSION     DAOQOC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8971_length_4331_cov_3.679607"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PL106_02335"
     CDS             <1..1087
                     /locus_tag="PL106_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNA31935.1"
                     /translation="DSLFLEGTGQVNIVQLGGSHVQADAWTARLRDRLQRTAPGTRAG
                     RGLIFPYNMAGSNNPYWYLPTYTGTWSAVKNTQRADSTQLGLAGYTITTRDPDTRLDI
                     CFRGSYEGHPFTRATVLHGADSSFRVSAWCADTGVIILWNTDVTLGRTTIGFSRAIDT
                     LHLRFEQVDTLAERSFTLRGIILGGEEPGIIYHALGVNGASTRSWLACPRLEEDLSLV
                     TPDLVVFSIGINDAHDPNFSAERYERNYDALIGKVRKVNPDAAVLLVTNSDSYRKRRY
                     PVRNAEAVRRTMLRLAEKHGCGVWDLYGVMGGQGSIRRWKRQGLARPDLVHFTRDGYT
                     LIGDLMFTAIMKSYGEHLTSTALTNGR"
     gene            1209..2876
                     /locus_tag="PL106_02340"
     CDS             1209..2876
                     /locus_tag="PL106_02340"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA31936.1"
                     /translation="MSELPAFLRLLFGYEEGLPLTFTQLFFWGFFVVVLAGFAVVHKR
                     IAVRNAWLFVASLFFYYKTSGLFVLILLFSTVSDFTIAHLIHRATSLSHKRLWLALSV
                     SINLLLLGYFKYAHFLVENINRAFGTHYVEINWFAQWGNGLFGTSHVVDRILLPVGIS
                     FFTFQAISYAVDVYRDEIKPVRGILDFGFFVSFFPQLVAGPIVRAAAFIPQVLAPYQL
                     SRRDFGLAVFWILNGLVKKVVIGDHIALNFIDRVFNDPMRYSGFECMMALFAYSLQVY
                     ADFSGYTDIAIGVALLMGFKLATNFNSPYKAVHVGEFWKRWHMSLSTWLRDYLYIPLG
                     GNRGASLGTWICLGLLVGVAVLLSGAFWLVPLALAVVALGVLLARWIPGFNNWTTTNA
                     NLMITMLLGGLWHGASWMFVIWGGLNGLALVFYKAWSKVSPWKHATGWAAHASAVLVT
                     LTFITFTRIWFRSPTLEVANSILHQIGYAFTLSVIPAVLIGFKSVFALMLAGYVIHWL
                     PTNFKQNYRERFADLPLWAMGLVCTGVVFMTYQFAAAGSVPFIYFQF"
     gene            complement(3202..3858)
                     /locus_tag="PL106_02345"
     CDS             complement(3202..3858)
                     /locus_tag="PL106_02345"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNA31937.1"
                     /translation="MNKLPLIGRIALTLGACLLVFFITREVYRDDTPEPATNSTVLLE
                     QVRKVMKLVTVEGDFNELYSREDNWNYARWLADMVPGFKKRVLLRVKARVSVGYDLSG
                     MTLTADERTRTVHLDLLDKPVILSLEHDIDYYDLDEGVFNHFAPHELSAIEADAKRRI
                     RDQVEEGGLFAKAGEQRAELVALVRALVEGKGWTFEEGTAVQDGRTRLKAEGGVLQGS
                     "
     gene            complement(3855..>4331)
                     /locus_tag="PL106_02350"
     CDS             complement(3855..>4331)
                     /locus_tag="PL106_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA31938.1"
                     /translation="GIGLGVLSAGLSAWFNIVNGVLVRRGDAVNIGLIELFSVMVCGA
                     VVLLAQGRAPQLPWQLSGADLGYQLLLGLVCTAFAFVTGVAVMKQLSPFSVVLAVNLE
                     PVYTIIIALLIWRQGEQLHAGSYLGLALILACLFANGLLQRRERIRQEATTIAPIA"
BASE COUNT          779 a         1391 c         1330 g          831 t
ORIGIN      
        1 ggacagcctg ttcctggagg gcaccggcca ggtgaacatc gtgcagctcg gtggctccca
       61 cgtgcaggcc gacgcatgga ccgcccgcct tcgcgaccgc ctccagcgga cagctccggg
      121 caccagggcg gggcgtgggc tgatcttccc gtacaacatg gccggcagca acaatccgta
      181 ttggtacctg ccgacgtaca ccggcacctg gagcgccgtg aagaacaccc aacgggcgga
      241 cagcacacaa ctgggcctgg cgggatacac catcaccaca cgggatccgg acacacggct
      301 cgacatctgc ttccggggca gttacgaggg ccatcccttc actcgtgcca ccgtgcttca
      361 cggggcggac agcagcttcc gtgtgagtgc ctggtgcgcc gatacggggg tcatcatcct
      421 gtggaacacc gatgtcacgc tcgggcgcac cacgatcggg ttcagccgcg cgatcgacac
      481 gctccacctc cgcttcgagc aggtcgacac gctggcggag cgttcgttca cgttacgtgg
      541 tatcattctc ggaggcgagg agcccgggat catctaccat gcgctcggtg tgaacggtgc
      601 ttccacacgc agctggctgg cctgccccag gcttgaggag gacctgtcat tggtgacccc
      661 ggacctcgtg gtcttctcca tcgggatcaa cgacgcgcac gatccgaatt tcagtgccga
      721 acgttatgaa cgcaactacg atgcgctcat cggcaaggtc cgcaaggtga accccgatgc
      781 ggccgtgctg ctggtgacca atagcgacag ttaccggaaa cgccgttacc ccgtgcgcaa
      841 tgccgaggcc gtacggcgta ccatgttgcg ccttgctgaa aagcacggat gcggcgtgtg
      901 ggacctgtac ggcgtgatgg gcgggcaggg ctccatacgc cgctggaagc gacaagggct
      961 ggcgcgtccc gacctggtcc atttcacacg tgatggctac accctgatcg gtgacctgat
     1021 gttcaccgcg atcatgaagt cgtacgggga gcatttgaca agcaccgcac tgacgaacgg
     1081 acgatgagcg cgcgagtgat gagcagcgag tggcgagtgg cgagtgaatg tccggccgga
     1141 tgccgcattc acgcgccact cgccactcct cactcgccac ccatcccagg acctccggca
     1201 caccacgcat gagcgagctc cccgccttcc tccgactgct cttcggatac gaggagggac
     1261 tcccgctcac cttcacgcaa ctgttcttct ggggcttctt cgtggtggtg ctggccggct
     1321 tcgccgtggt acacaagcgg atcgccgttc gcaatgcctg gctcttcgta gccagcctct
     1381 tcttctacta caagaccagc gggctcttcg tgctcatcct gctcttcagc acggtcagcg
     1441 acttcaccat cgcgcacctc atccaccgtg cgacaagtct gtcccacaag cggctgtggc
     1501 tcgccctgag cgtatcgatc aacctgctgc tgctgggcta cttcaagtac gcgcacttcc
     1561 tggtcgagaa catcaatcgc gcgttcggca cccactacgt ggagatcaac tggttcgcgc
     1621 agtggggcaa cggcctgttc ggcacctcgc acgtggtgga ccggatcctg cttccggtgg
     1681 gcatcagttt cttcaccttc caagccatca gttacgcggt ggacgtgtac cgggacgaga
     1741 tcaagccggt gcgcggcatc cttgacttcg ggttcttcgt gagttttttc ccgcagctgg
     1801 tggcgggtcc catcgtgcgc gccgcagcct tcatcccgca ggtcctggca ccgtaccagc
     1861 tgagccgccg ggacttcggg ctggccgtgt tctggatatt gaatggtctg gtgaagaaag
     1921 tagtgatcgg cgaccacatc gcgctcaact tcatcgaccg ggtattcaac gacccgatgc
     1981 gatattcggg tttcgagtgc atgatggcct tgttcgcgta ctcgttgcag gtttatgcgg
     2041 acttcagcgg ctacacggac atcgccattg gcgtggccct gctcatgggc ttcaagctgg
     2101 cgaccaactt caacagtccc tataaagcgg tacatgtggg cgaattctgg aagcgctggc
     2161 acatgtcgtt gagcacctgg ctgcgcgact acctgtacat cccgttgggc ggcaaccggg
     2221 gcgcctcgtt ggggacctgg atctgcctgg ggctgcttgt gggcgtggcc gtgctgttga
     2281 gcggcgcgtt ctggctggta ccgctggccc ttgcggtggt ggccctgggc gtgctgctgg
     2341 cccggtggat ccccggcttc aacaactgga cgaccaccaa cgcgaacctg atgatcacca
     2401 tgctgttggg tgggctatgg cacggcgcca gttggatgtt cgtgatctgg ggcggattga
     2461 acgggctggc cctggtgttc tacaaggcgt ggagcaaggt gagcccgtgg aaacacgcga
     2521 cgggatgggc ggcacacgct tcggccgtgc tggtcacgct caccttcatc accttcacgc
     2581 gcatctggtt ccgcagcccg acgctggagg tggcgaacag catcctgcac cagatcggat
     2641 acgcattcac gctgtcggtg atccccgccg tgctgatcgg tttcaagagc gtgttcgcac
     2701 tgatgctcgc gggctacgtg atccattggt tgccgaccaa cttcaaacaa aactaccggg
     2761 aacgcttcgc cgatctgccc ttgtgggcga tgggacttgt ctgtaccggc gtggtgttca
     2821 tgacgtacca gttcgcggcg gcggggagcg tgccgttcat ctatttccag ttctgagtgc
     2881 agggccacca gttgctcagc cccgtccgcc agttgctctt cagcgctcac ccttgttcgc
     2941 gggttgtgct tgtttcgcct tcacgacgac agttcaacac caacacccca acaacaccgg
     3001 ccttcgggcc cgagagacac caccacccgt tgcggcccgt aagccgccgg gagccaaggc
     3061 caccaaggag cgccgtaacg ccccaccaac ggagccttcg ccacaccaag gatgccccgg
     3121 ccatcgtacc ggagcgcatc catcgagcag ggagcccgga gcgccgtaac gcaccgggct
     3181 tccgctttga cggggtgcgg gtcaggaccc ctggaggaca cccccctccg ccttcagccg
     3241 ggtccgacca tcctggacgg ccgtgccttc ctcgaaggtc caacccttgc cctcgaccag
     3301 ggcgcgcacc agggccacca gctcggcccg ctgctccccc gctttggcga aaagtccgcc
     3361 ctcctccacc tggtcgcgaa tgcgacgttt ggcgtcggct tcgatcgcgg aaagctcatg
     3421 cggggcgaag tgattgaaga cgccctcgtc caggtcgtag taatcaatgt cgtgctccag
     3481 ggataatatg accggcttgt cgagcaggtc caggtgtacc gtacgggtcc gctcgtccgc
     3541 ggtaagcgtc atgccggaca ggtcgtagcc gacgctcaca cgcgccttca cgcgcaggag
     3601 tactcgcttc ttgaatccag gtaccatatc ggccagccac cgcgcatagt tccagttgtc
     3661 ctcgcggctg tacagctcgt tgaagtcgcc ttccacggtg acgagcttca tcaccttgcg
     3721 cacttgctca aggagcaccg tgctgttcgt tgcgggttcc ggggtatcgt cgcgatacac
     3781 ctcacgagtg atgaagaaga cgagcaggca ggcgccgagc gtgagtgcga tgcgaccgat
     3841 caagggcaac ttgttcatgc gatgggcgcg atggtggtgg cctcctgccg tatgcgctcg
     3901 cgacgttgca ggaggccatt ggcgaacaga caggcgagta tgagcgcgag gccgaggtat
     3961 gatccagcgt ggagttgctc gccctgccgc cagatgagca gtgcgatgat gatggtgtac
     4021 accggttcga ggttcaccgc gagcacaacg gagaatggtg aaagctgttt catcacggcc
     4081 acgccggtaa cgaaggcgaa tgcggtgcag accagaccaa ggagcaattg atacccgagg
     4141 tccgccccgg acagctgcca ggggagctgc ggtgcccgcc cctgcgccag caggaccacc
     4201 gcaccgcaca ccatgacgga gaacaactcg atgaggccga tgttcacggc gtccccgcgt
     4261 cgcaccagga cgccgttcac gatgttgaac cacgcgctca gaccagcgct cagcacaccc
     4321 aggccgatgc c
//
LOCUS       DAOQOC010000091         4325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8987_length_4325_cov_3.482436, whole genome shotgun sequence.
ACCESSION   DAOQOC010000091 DAOQOC010000000
VERSION     DAOQOC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8987_length_4325_cov_3.482436"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PL106_02355"
     CDS             complement(<1..1436)
                     /locus_tag="PL106_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNA31939.1"
                     /translation="MNTKLLSGVLSAFLFTCSGTMVLAQKNGERRVRIEITRNENGNE
                     SHVTREFDLNDGQQLADALRELGVMDELNVIGDDENLVLDLRRMRDGGLLNDMSMALR
                     MADPDAVAQPRAYLGVYYGNWNESCDKEARKKDPPVKEGACITAVESGTPAEQAGLKE
                     GDVIVAMDDRVITSGAGLLDAIRAHDPGDNVKLIYYRGKEKRSTTVALAARDNGPDVM
                     SWNWHTDSAAYERAMEAFGRAMQDMALDSEARFGGAFLGVVGEDLPNNGGVRVTSVSD
                     SSAAQRMGLKEGDAITAINGDKIDNFEDLIDHLADAQPGSKATVDVLRDGRAIVLNGE
                     LGERTGAFPMYGGPSGAPFMFPMPNIPDMPYPYSEDGMEEDANGAGQRAEHDHLRREM
                     DELRREMDRLRRDLRGDVIREMRVTVEAVELSPEESTALKNKGVSTLDRSLDLTGLRI
                     APSPTDGAFDIAFQVPERGDLNVDVHDAG"
     gene            complement(1533..1925)
                     /locus_tag="PL106_02360"
     CDS             complement(1533..1925)
                     /locus_tag="PL106_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNA31940.1"
                     /translation="MSWQALALMRIDKFLWCVRLYKSRSLATEACASGHVRMGDREVK
                     ASAVIQVRDLFSVRQAPIWRSFEVIALPSTRVGAKLVPGLITDRTSWEDLEKQETARK
                     VKAAQRDPGSGRPTKRERRKIDRFTRDD"
     gene            complement(2019..2095)
                     /locus_tag="PL106_02365"
     tRNA            complement(2019..2095)
                     /locus_tag="PL106_02365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2059..2061),aa:Arg,seq:ccg)
     gene            complement(2145..3089)
                     /locus_tag="PL106_02370"
     CDS             complement(2145..3089)
                     /locus_tag="PL106_02370"
                     /inference="COORDINATES: protein motif:HMM:NF028494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31941.1"
                     /translation="MCPGYPLFFRTVVGIGVFATVLSSCVDRPTTDAAARATPTDTTW
                     TGEMRMTAGDTSIRICGTGHVYHLTGPAMDTIVQRYTGARMRTGQRMKLWVSGHFGTV
                     ERNGLVDSVLHATKFQHLDASLRCDPVPEARVSGDWKLDDVDPHRPRDIHVHLYTNGT
                     ARMITDLRNGQSPFEEDGSWGVDGEGAVQVNWPLRAQTMSLQWEPGILIGTDRRPGMR
                     VTLHRVGPADPMAGVFGRTARWLAASATALGKPTSPESIVAATRLDTLFATPEAQHIL
                     RAQALDTLQPGVQEAAVRLDAVRTAHDLLLLMRIASRH"
     gene            3267..>4325
                     /locus_tag="PL106_02375"
     CDS             3267..>4325
                     /locus_tag="PL106_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNA31942.1"
                     /translation="MTELRNIAIIAHVDHGKTTLVDRILHQCQLFRENQSSGELILDN
                     NDLERERGITILSKNVSVRYKGVKINVIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GAMPQTRFVLGKAIQLGLKPIVVINKVDKPNCTPEEVHEQVFDLMFALDATEEQLDFP
                     VVYGSSKQGWMGPDWKTPTEDVSHLLDVILEKIPAPKKVDGTLQMRITSLDYSSFQGR
                     IAVGRVSRGSIKAGQNVSLVKNDGTVKKGKVAELMVFEGLGKEKVKDRELTSGELCAV
                     MGLESFDIGDTIADFENPEGLTPIKVDEPTMSMLFTINNSPFFGREGQFVTSRHVRDR
                     LFKEIEVELSEDQKRVYKL"
BASE COUNT          794 a         1322 c         1400 g          809 t
ORIGIN      
        1 ccggcatcat gcacgtccac gttcaggtcg ccccgctccg gtacttggaa tgcgatatca
       61 aaggcaccgt cggtgggcga gggcgctatg cgcaggccgg tcaggtccag tgacctgtcc
      121 agggtggaga cccctttgtt cttcagtgcg gtgctttctt cgggtgagag ttcaacggcc
      181 tcaacggtca ctcgcatctc acggatcaca tcaccccgca gatcgcggcg caggcggtcc
      241 atctcgcggc gcaactcgtc catttcgcgc cgcaggtgat cgtgttccgc acgttgtccg
      301 gcaccgttgg catcttcttc cataccgtcc tcgctgtagg ggtagggcat atcggggatg
      361 ttgggcatgg ggaacatgaa cggggcaccg gacgggccgc cgtacatggg gaatgcgccc
      421 gtccgttcgc ccagttcgcc gttcagtacg atcgcccggc catcgcgcag cacgtccacc
      481 gtggccttcg atccgggctg cgcatcggcc aggtgatcga tcaaatcctc gaaattatca
      541 attttatccc cgttgatcgc ggtgatggcg tccccttcct tcaagcccat gcgctgggcc
      601 gcactgctgt ccgaaacgct ggtgacgcgc acgccgccat tgttgggcag gtcctcgccg
      661 accacaccca ggaaggcgcc accgaagcgt gcctcgctgt ccaaggccat gtcttgcatc
      721 gcgcggccga aggcttccat ggcgcgctca tacgcggcgc tgtccgtgtg ccagttccag
      781 ctcatcacat cgggcccgtt atcgcgggcc gcaagggcga cagtggtgga acgcttctcc
      841 ttgccacggt agtaaatgag tttcacgttg tcgccgggat catgcgcacg gatggcgtcc
      901 aatagcccgg caccactggt gatcacccgg tcgtccatgg cgacgatcac atcgccctct
      961 ttcaggcccg cctgctcggc cggggtgccg ctttccacgg ccgtgatgca ggctccttcc
     1021 ttcaccggag gatctttctt gcgggcctcc ttgtcgcagc tttcgttcca gttgccatag
     1081 tacacgccca ggtaggcccg gggttgcgca acggcatccg gatcggccat gcgcagggcc
     1141 atgctcatgt cgttgagcag accgccatcg cgcatgcgcc gcagatcgag caccaggttc
     1201 tcatcgtcgc cgatcacgtt caactcgtcc atcacgccga gttcacgcag ggcatcggcg
     1261 agttgttgcc catcgttcag gtcgaattcg cgggtgacat ggctttcgtt gccgttctcg
     1321 ttgcgtgtga tctcgatacg cacgcgccgc tcaccgttct tctgtgccag gaccatggtg
     1381 ccggaacagg tgaacaggaa cgcactgagg acgccggaaa ggagcttcgt gttcatggct
     1441 tcgaccgttg atgcatgcga aactatgggg gccttgtgcg gtgccgctgt taaggcctgc
     1501 taaaagctgc caagcggcgt aaaggctgta cctcagtcgt cccgggtgaa gcggtcgatc
     1561 ttgcggcgct cacgtttggt ggggcgtccg gagccggggt cgcgttgtgc ggccttcacc
     1621 ttacgggcgg tctcctgctt ctccaggtcc tcccacgatg tacggtcggt aatgagcccc
     1681 ggaacgagtt tggcaccgac cctggtggag ggtagggcga tcacttcaaa gctgcgccag
     1741 atgggggctt ggcgcacgga gaacaggtcc ctcacctgga tcaccgcact ggctttcacc
     1801 tcgcgatcgc ccatgcggac gtgcccactg gcgcaggctt ccgtggccag actacgcgac
     1861 ttgtagagcc tgacgcacca gaggaacttg tcgatgcgca tcagtgcgag ggcttgccag
     1921 gacatgttcc gatccgatcc tgcagccatg tcaggggcac atggtcggat gaacggagga
     1981 acacatgaaa aaagcggtcc gcctttcgga gaaccgcttg gtgagcccgg agggattcga
     2041 acccccaacc aacagaaccg gaatctgtca ttctatccag ttgaactacg ggccctgaag
     2101 agccccggaa ttttatccgg ggggccgcga agataggctg cacttcaatg gcgcgatgcg
     2161 atgcgcatca gaagcagcag gtcgtgggcg gttcggaccg catcgagtcg caccgccgct
     2221 tcctggacac cgggttggag ggtatccaac gcttgggcgc gcaggatgtg ttgtgcctca
     2281 ggcgtggcga agagcgtgtc gaggcgcgtg gctgctacga tcgactcggg gctcgtgggt
     2341 ttgcccagcg cggtggccga agccgccagc caccgcgcgg tacgtccgaa gacacctgcc
     2401 atggggtctg cggggccaac acgatgaagc gtcacgcgca tgccggggcg ccgatccgtg
     2461 ccgatgagta tgcccggttc ccattggagt gacatggttt gcgcgcggag gggccagttc
     2521 acctgtaccg cgccctcgcc atccacgccc catgaaccgt cctcctcgaa aggtgactgg
     2581 ccgttgcgca ggtcggtgat catgcgtgct gtaccgttgg tgtacaagtg aacgtggatg
     2641 tcgcgtggcc ggtgcggatc cacatcatcc agcttccaat cgcccgacac gcgcgcctcg
     2701 ggaacagggt cgcagcgcaa ggatgcgtcc aggtgctgga atttggtcgc gtggagcacg
     2761 ctgtcaacaa ggccattgcg ttccacggtg ccgaagtggc ccgacaccca cagcttcatg
     2821 cgttggccgg tgcgcatgcg ggcgccggtg tagcgttgta cgatggtgtc catggccggt
     2881 ccggtcaggt ggtacacatg gccggtgccg cagatccgga tggaggtatc gcccgcggtc
     2941 atgcgcattt cgcccgtcca cgtggtatcg gtcggggtgg ccctggcggc tgcgtccgtt
     3001 gtgggccggt ccacgcaact gctgagcaca gtggcaaaaa caccgatgcc gacaaccgtg
     3061 cggaagaaga gcgggtaccc tggacacatg gggcgaacat aggccacgcg caccgggcct
     3121 gacgggtcag aaggatccga cgcgttcggg agcctgcttt tcaatacgcc gatggactac
     3181 cttcgcgccc cttttcgcga gggcatcctt ccgggggcgg ccagagcgaa caaggaatgc
     3241 cccggagccc atcgaagatc gacaccatga ccgaactgcg caacatcgcc atcattgccc
     3301 acgtggacca cggcaagacc accctggtgg accggatcct gcaccaatgc cagcttttcc
     3361 gggagaacca gtccagtggc gagctgatcc tcgataacaa tgatcttgag cgtgagcgcg
     3421 gcatcaccat cctgagcaag aacgtgagcg tgcggtacaa gggcgtgaag atcaacgtga
     3481 tcgatacccc gggccacagc gacttcgggg gggaggtgga gcgcgtgctc aacatggccg
     3541 atggcgtgct gctgctggtg gacgccttcg agggggcgat gccgcagacg cgtttcgtgc
     3601 tgggcaaggc cattcaactc gggttgaaac cgatcgtggt gatcaacaag gtggacaagc
     3661 cgaactgtac gccggaggag gtgcatgagc aggtgttcga cctgatgttc gcgctggacg
     3721 ccaccgagga gcagctcgat ttccccgtgg tgtacggcag cagcaagcag ggctggatgg
     3781 gcccggactg gaagaccccc accgaggacg tgagccacct gctggacgtc atcctggaaa
     3841 agatccctgc gcccaagaaa gtggacggca ccctgcagat gcggatcacc tcgctcgatt
     3901 acagcagctt ccagggccgc atcgccgtgg ggcgcgtgag caggggcagc atcaaggccg
     3961 ggcagaacgt gagtctggtg aagaacgatg gcacggtgaa gaagggcaag gtggccgagt
     4021 tgatggtctt cgagggcctg ggcaaggaga aggtgaagga ccgcgaactc accagtggcg
     4081 aactctgtgc ggtgatgggg ctggagagct ttgacatcgg ggacaccatc gccgacttcg
     4141 agaaccccga gggcctcacg cccatcaagg tggacgagcc caccatgagc atgctcttca
     4201 ccatcaacaa ctcgcccttc ttcggccgcg agggccagtt cgtcaccagc cgccacgtgc
     4261 gcgaccgcct gttcaaggag atcgaggtcg agctgtccga ggaccagaag cgcgtctaca
     4321 agctg
//
LOCUS       DAOQOC010000092         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_8993_length_4323_cov_3.572399, whole genome shotgun sequence.
ACCESSION   DAOQOC010000092 DAOQOC010000000
VERSION     DAOQOC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8993_length_4323_cov_3.572399"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1253)
                     /locus_tag="PL106_02380"
     CDS             complement(123..1253)
                     /locus_tag="PL106_02380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNA31943.1"
                     /translation="MSQLTRAQREALKKPKEQAPAPLFPEDQVEGEDGGEQELYEHHR
                     IVCDRGQGLMRLDKFLFERLPSISRNRIANAAKAELVRVNGKPQKPSYKVKPFDDISM
                     VLPWPVREVELTPENIPLTVLYEDDHMLVIDKQAGLVVHPGHGNWSGTLVNALMYHFG
                     QQPKRVGEMDARPGLVHRLDKNTSGVMVIGKTEEAAAQLANQFFHRTNDRRYLALVWG
                     DPAEDEGTIEGHIGRSPKDRTVMQVFPEGEHGKPAITHWKVVERFVYCTLVECKLETG
                     RTHQIRAHMRYLGHQLFNDPEYDGDRVVKGTVFTKYKQFVMNAFELCPRQALHAAVLE
                     IDHPVNGKRMRFEQPLPPDMAAVVEKWRKYAASGIGQEGGEE"
     gene            complement(1250..3481)
                     /locus_tag="PL106_02385"
     CDS             complement(1250..3481)
                     /locus_tag="PL106_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31944.1"
                     /translation="MALFLLSGATAQRIGPERVEPLHARPAKEVRPMLRSGNLHEWFI
                     YAYQPQTIPVVDDFSVDRTRHLDADSDDPGVTLTETVYHLEVLGVSTPDMVFMFQAAT
                     RLTVDIQPDTVILTTDTLEPVNVRVFDISTYPATWEDHEMWPPYVLRDTVGDPTTDTT
                     YLAPDLVQDSLLVYTVPPADGTYLNPDNTNVPLILWESDHAYVNGTYPIDPPTIGVAS
                     FDGMDRDGWPYEAENPNVEGIADVMTSVPLNWPPGTEPADSVYLSFFYQPQGRSGDSD
                     VDPTDSLRVEFYSPGQDSWFLEWATPYTALHPFEQAMVPITYAGYLEDGFRVRFSNRA
                     TLGGAVDHWHVDYVRIGLNRSVTDTLLQDVAYVYPANTLLSPWTSVPLAKYNADPAHY
                     MAASVEQTQCNRYNVDALITYGHEMTTDCGNSASRDDGGNVFGNALTTFTSSHAINSG
                     AQPYTYDLGACPGAAFATVKFWTMANPDALRHNDTTSFTQEISNYYSYDDGSAELGYS
                     LNTNGGRIAYRFDTQGQDSLRAVRMYFDPIFTYGDIPNDPRDGSFLLTVWQDLNSAPI
                     YQNISFSNPEYRPWGPDHFVEYALDSTIAVGGTFYVGWTQTNAVRMNLGMDMNRVNND
                     RMFFNVGNVWQQSAIAGSWMIRPVMVSAVDPFASVPEADEVVMTLFPNPATDEVRLRV
                     PGETPASIELYDATGRRVASERYRTDGVLSIGNVVDGLYVVRALDDNGRSIAQARLVV
                     QRP"
     gene            complement(3492..>4323)
                     /locus_tag="PL106_02390"
     CDS             complement(3492..>4323)
                     /locus_tag="PL106_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNA31945.1"
                     /translation="RVGSKVTKRTILNLLLPVGVAVLLLLGAWVWLNVYTRHDARVTV
                     PDLAGLTADEAAERLASVGLRTEVIDSVYNDEMPKGSVVDQDPDAEQVVKPDRTIYLV
                     MNASEAKMLDMPQLVNLSKRQAISVLDILGIKVKELQYRPDPCVDCVVAQLYKGKPIA
                     AETRIRRGESITLVLGSGTNGERVQVPDVRGMGLAELKAVLNMASLNLGVVAACEGCT
                     TSADTAMARVERQLPEPYRNNMIGAGGVIDVWLTTDTVGLRHPEPVDSTTNDLDDEDN
                     "
BASE COUNT          737 a         1324 c         1394 g          868 t
ORIGIN      
        1 gtactactcg cacttcccgc agaagcaccg ggtgatcgtg accagcagcg aggcggtggc
       61 gcgcgcctgg ctgaaagacc ggctcccgga gttgtccggt tcgccaagct gaacaagtag
      121 gctcactcct cgcctccctc ctggccgatc ccgctggccg cgtatttccg ccatttctcc
      181 acaacggcgg ccatatcagg cggcagtggt tgttcgaaac gcatccgctt gccattgacg
      241 ggatggtcga tctccagcac cgcagcgtgc aacgcctgcc gcggacagag ttcgaacgcg
      301 ttcatcacga actgcttgta cttggtgaag accgtgccct tcaccacgcg gtcgccatcg
      361 tactcgggat cgttgaagag ttgatggccc aggtagcgca tgtgcgcacg gatctggtgg
      421 gtgcggcccg tttcgagctt gcactccacc agcgtgcagt agacgaaacg ctccaccacc
      481 ttccagtgcg tgatcgcggg cttgccatgc tcaccctcgg ggaagacctg catcacggtg
      541 cggtccttgg gtgagcgacc gatgtgtccc tcgatggtgc cctcgtcctc ggcgggatca
      601 ccccacacca gggccagata gcgacggtcg ttggtacgat ggaagaattg gttcgcgagc
      661 tgtgccgcag cctcctcggt cttgccgatc accatcacac cgctggtgtt cttgtccagt
      721 cggtgaacaa gaccgggccg ggcatccatc tcgccaacac gcttgggctg ctgaccgaaa
      781 tggtacatga gcgcgttgac gagcgtgccg gaccagttgc catggccggg atgcaccacc
      841 aggccggcct gcttgtcgat caccagcatg tgatcatcct cgtacagcac ggtgagcggg
      901 atgttctcgg gcgtcaactc cacctcgcgc accggccagg gcagcaccat gctgatgtcg
      961 tcgaagggct tcaccttgta gctcggcttc tggggcttgc cgttcacgcg caccaattcg
     1021 gctttggcag cgttggcgat gcggttgcgg ctgatgctcg gcagacgctc gaagaggaac
     1081 ttgtccaggc gcatgaggcc ctgacccctg tcgcaaacaa tacggtggtg ctcgtacaat
     1141 tcctgctcgc cgccgtcctc cccttccacc tggtcctcgg ggaagagcgg cgccggtgct
     1201 tgctccttgg gcttcttgag cgcttcgcgt tgcgccctgg tgagctggct catgggcgtt
     1261 gcaccacgag gcgtgcctgt gcgatggaac gaccgttgtc gtcgagtgcg cgtaccacgt
     1321 acaggccgtc aacgacattc ccgatggaaa ggacaccatc ggtccggtag cgttcgctgg
     1381 ccacgcgacg accggtggcg tcgtacagtt cgatggatgc gggcgtttcc ccgggcacgc
     1441 gcaggcgcac ctcatccgtg gccggattgg ggaagagggt catgaccacc tcgtcggcct
     1501 ctggtaccga tgcgaaggga tccacggcgc tcaccatcac cgggcggatc atccagctgc
     1561 cggcgatcgc gctctgctgc cacacgttgc ccacgttgaa gaacatgcgg tcgttgttca
     1621 cgcggttcat gtccatgccg aggttcatac gcaccgcgtt ggtctgcgtc catccgacgt
     1681 agaaggtacc gcccacggcg atggtgctgt ccagcgcgta ctccacgaag tggtccggtc
     1741 cccaggggcg gtactcgggg ttgctgaaac tgatgttctg gtaaatgggc gcgctgttga
     1801 ggtcctgcca cacggtgagg agaaaacttc cgtcgcgcgg atcgttgggg atgtccccgt
     1861 aggtgaagat gggatcgaaa tacatgcgca ccgcacgcag cgagtcctgt ccctgggtat
     1921 cgaagcggta agcgatgcgg ccgccgttgg tgttcaacga gtaaccgagt tcggcactgc
     1981 cgtcatcgta actgtagtag ttgctgatct cctgggtgaa ggaggtggta tcgttgtggc
     2041 gcagtgcatc gggattggcc atggtccaga acttcaccgt ggcgaaagcg gcaccggggc
     2101 aggccccaag gtcgtaggtg tatggctgcg ctccactgtt gatggcgtgc gaggaggtga
     2161 aggtggtgag cgcgttgccg aacacgttgc ccccgtcgtc gcgcgaggcg ctgttgccgc
     2221 aatcggtggt catctcgtgg ccgtaggtga tcagcgcatc cacgttgtag cggttgcact
     2281 gcgtctgctc cacgctggcc gccatgtagt gcgcgggatc ggcgttgtac ttggccagcg
     2341 gtaccgatgt ccagggggag agcagggtgt tggcgggata cacgtaggcc acgtcctgca
     2401 gcagcgtatc ggtcacactt cggttcaggc cgatacgcac atagtccacg tgccagtggt
     2461 ccacggcccc cccgagcgtg gcgcggttgc tgaagcgtac gcggaagcca tcctccagat
     2521 acccggcata ggtgatcggc accatggctt gttcgaaggg atggagcgcc gtatagggtg
     2581 tcgcccattc caggaaccag gaatcctggc cgggcgagta gaattccacg cgcaggctgt
     2641 cggtgggatc cacatcgcta tcgccactgc ggccctgggg ctgatagaag aagctgaggt
     2701 agacgctgtc ggcgggttcg gtaccgggcg gccagttgag gggcacggag gtcatcacgt
     2761 cggcgatgcc ttccacattg gggttctccg cctcgtacgg ccagccgtcg cggtccattc
     2821 catcgaagct ggccacgccg atggtgggcg gatcgatcgg gtaggtgccg ttcacgtagg
     2881 cgtggtcact ttcccagagg atgagcggga cgttggtatt gtccggattg agataggtgc
     2941 cgtcagcggg cggaacggtg tacacgagga gcgaatcctg caccaggtcc ggtgccaggt
     3001 aggtcgtgtc cgtggtgggg tcgcccacgg tatcgcgcaa cacgtacggc ggccacatct
     3061 cgtgatcctc ccacgtggcc gggtaggtgc tgatgtcgaa cacgcgcaca ttcaccggtt
     3121 ccagggtatc ggtggtcagg atcaccgtat cgggctggat gtccacggtg agccgcgtag
     3181 ccgcctggaa catgaagacc atgtcagggg tactgacgcc gagcacctcc aggtggtaca
     3241 ccgtttcagt gagggtgacc ccgggatcat cgctgtcggc atccaagtgg cgcgtgcgat
     3301 ccacggaaaa atcatccacg accgggatgg tttgtggttg gtacgcgtag atgaaccact
     3361 catgcaggtt cccgctgcga agcatgggcc tcacctcctt tgccggtctg gcgtgcaagg
     3421 gttccacccg ctcgggaccg atgcgctggg ccgtggctcc tgatagcagg aacagggcca
     3481 tggcgaatgt gctagttatc ctcatcgtca aggtcgttgg tggtgctgtc caccggttct
     3541 ggatggcgca ggccgacggt gtcggtggtg agccacacat cgatgacgcc gccggcgccg
     3601 atcatgttgt tcctgtacgg ctcgggcaac tggcgttcca cgcgggccat ggccgtatcg
     3661 gccgatgttg tgcaaccctc acaggcggct accacgccca ggttgagcga ggccatgttg
     3721 agcaccgcct tcagttccgc caggcccatg ccgcgtacat cgggcacctg cacgcgctcc
     3781 ccgttggttc ccgagcccag caccagggtg atggattccc cgcgccggat gcgcgtttcc
     3841 gccgcgatgg gtttgccctt gtacagttgt gccaccacac aatccacgca gggatcgggc
     3901 cggtactgga gttccttcac cttgatcccg aggatgtcca gcacgctgat cgcctggcgt
     3961 ttgctcaggt tcaccaactg gggcatgtcg agcatcttgg cctcgctggc gttcatcacc
     4021 aggtagatgg tgcggtcggg cttcaccacc tgttccgcgt cgggatcctg atccaccacg
     4081 gaacccttgg gcatctcatc gttgtacacc gaatcgatca cttcggtccg cagacccacc
     4141 gatgccaagc gttccgccgc ttcatcggcc gtaagccccg ccaggtccgg cacggtcacg
     4201 cgtgcatcat ggcgtgtgta cacgttgagc cagacccaag cgcccaacag cagcagcaca
     4261 gccaccccca cgggcagcaa caggttcaag atcgtgcgtt tcgtcacctt gcttccaacg
     4321 cgg
//
LOCUS       DAOQOC010000093         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9076_length_4300_cov_3.645701, whole genome shotgun sequence.
ACCESSION   DAOQOC010000093 DAOQOC010000000
VERSION     DAOQOC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9076_length_4300_cov_3.645701"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PL106_02395"
     CDS             complement(<1..697)
                     /locus_tag="PL106_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNA31946.1"
                     /translation="MDLKEPLPASGIAALIGAAVRGNAQRMVTGINEINRVREGDLLF
                     VDHPKYYAKALNSAASVVLINKDDVAIPEGKAIILCTDPCTEYNKLVVHFSPRTGWNG
                     AAPSIGEHTEVHPSAVIGPEVRIGRDCRIMPGVVIYGPATIGDRVILHANTVLGADPF
                     YYKKRPTGHDKMPPCGSVVIEDDVEIGALCTIDRGVSADTRIGKGTKLDNHVQVGHDT
                     LIGAHCLICAHVAI"
     gene            complement(900..1463)
                     /gene="efp"
                     /locus_tag="PL106_02400"
     CDS             complement(900..1463)
                     /gene="efp"
                     /locus_tag="PL106_02400"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNA31947.1"
                     /translation="MASTSDVNIGSYIRFNNDICQIMEWQHRTPGNLRAFYQGKMRNL
                     RNGKLNEHRWRSGETMEVLRVEIHELQYLYREGDNLVLMNPNTFEQLYVPAALFGDAM
                     GFMKEEMIMQVGFESETPIFARPPKVVELAVTYTENVVKGDTSNKVMKDAKLETGAEV
                     QVPSFVEIGTVVRIDTETGEYLDRVKK"
     gene            complement(1584..3212)
                     /locus_tag="PL106_02405"
     CDS             complement(1584..3212)
                     /locus_tag="PL106_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31948.1"
                     /translation="MKATYGLVFVVVASVTANAQQLHQVIWDTLDTDLHPTVRYAGFS
                     DDGGAVVALRTSHGTDLVKTAADGTLVWRKDIGLPSNAYGQIFSNGMGGHLHLFNDSS
                     YWEPLDLDTVMATLSLRMTSLNSTGEPLWSHRILSSITSMAWMPTAPTYDAVQSGTGD
                     LFVIGSFSSSSDTQVMVLRIGLDGAVIWARRVPVMDSPLNNTSYILVPDGIGGVYLSY
                     NTFMGSVTKVARLSAVGDLVWAKDIWLENNIFDVQAQGGALMSNGDLCIIGNAMVGAD
                     SKGFHFVLTPAGAWQRADLYGETWMGPAIGQVGDTLLMMMGSSVTRMSPTFDFLDLAG
                     AVGATGMNATHALAATKVKYENGTVHVIGNLNSTDLTFGYTTYRPADWSFLFDPTSGC
                     AIASYPLGVSHDQVPESLLADEVLTGTSEPIPTTVQTMSITAVDRPLWTFTDACTVIS
                     EAPDGVSEAEHVSAIVLANNPASNGSPVMVTCRVPAVLDLYDARGARVIVGVRATADT
                     PMAIDVSGLAPGIYPLLARQLNGQLVATRKVIVQ"
     gene            complement(3297..3911)
                     /locus_tag="PL106_02410"
     CDS             complement(3297..3911)
                     /locus_tag="PL106_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA31949.1"
                     /translation="MRITLQNVSKSFGREHVIRDLSHVFEPGSRTALLGPNGSGKSTL
                     LQLIGGISIPTRGTVTHEHDGPTVDPDLVYRHISIAAPYMSLYEELSLRETITSHKRF
                     KPVRGALTAEEVARIALLDKHMGKPVRDLSSGMKQRLKLALAVLSDTSLLLLDEPTSN
                     LDKDGSAWFRDLLTKNLDARTLVVASNRQAEETFACDRSVEVGQ"
     gene            complement(3956..>4300)
                     /locus_tag="PL106_02415"
     CDS             complement(3956..>4300)
                     /locus_tag="PL106_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNA31950.1"
                     /translation="LEGNVAVQQFIHIGAHSFIAGASLVRKNVPPFVKAAREPLSYIG
                     VNVVGLRRRGFNDEQVARIEDIYREIFVRNTNVDRAVQNVLQTMPRSHERGVIVDFIN
                     NSPKGIMRGLAE"
BASE COUNT          790 a         1323 c         1287 g          900 t
ORIGIN      
        1 caatggccac gtgcgcgcag atcaggcagt gcgccccgat cagcgtgtcg tgccccacct
       61 gtacgtggtt gtccagtttg gtgcccttgc cgatgcgcgt gtcagcgctc acgccccggt
      121 cgatggtgca gagcgcgccg atctccacgt cgtcctcgat caccacgctt ccgcagggcg
      181 gcatcttgtc gtgtcccgtg ggccgtttct tgtagtagaa gggatcggcc cccagcaccg
      241 tgttggcgtg caggatcacc cggtcgccga tggtggccgg tccgtagatg accaccccgg
      301 gcatgatccg gcagtccctg ccgatgcgca cctcgggacc gatgaccgcc gaaggatgca
      361 cctcggtgtg ctcaccgatc gacggtgcgg caccgttcca tcctgtgcgc ggactgaaat
      421 gcactaccag cttgttgtat tcggtacagg gatccgtgca gaggatgatg gccttgcctt
      481 cgggtatggc cacatcgtcc ttgttgatga gcaccaccga tgccgcgctg ttcagtgcct
      541 tggcgtagta tttcggatgg tccacgaaga ggaggtcgcc ctcgcgcacc cggttgatct
      601 cgttgatgcc ggtgaccatg cgctgcgcat tgccccgtac cgcagcgccg atcagcgcgg
      661 cgatcccgga ggcgggcagg ggttccttca ggtccatcgg ccgcgaaggt accccggcga
      721 tttccttgtt gggatacggg aacgggaaac gacgaacccc gtggtgtggc cacggggttc
      781 gtccattgat ccgggcgacg tgaccgtcgg ccctgcgatc atttttcaca cggtccaaat
      841 gcggacgacg caagcgtctt cttcacatgg tccgggggtg gacgacgcaa gcgtcgtccc
      901 tactttttca cgcggtccag gtactcgccg gtctcggtgt cgatgcgcac caccgtgccg
      961 atctccacga agctcggcac ttgtacctcg gcgccggtct ccagcttggc gtccttcatc
     1021 accttgttgc tggtgtcgcc tttcaccacg ttctccgtgt aggtgacggc cagttccacc
     1081 actttcggtg ggcgggcgaa aatgggcgtc tcgctctcga agcccacctg catgatcatc
     1141 tcctccttca tgaagcccat ggcatcgccg aagagggccg ccggcacgta cagctgctcg
     1201 aaagtgttgg ggttcatcag taccaggttg tctccttcac ggtagaggta ttgcagttcg
     1261 tggatctcca cgcgcagcac ttccatggtc tcaccggaac gccagcggtg ctcgttgagc
     1321 ttgccgttgc gcaggttgcg catcttgccc tggtagaacg cccgcaggtt gcccggcgtg
     1381 cggtgctgcc attccatgat ctggcagatg tcgttgttga agcggatgta cgatccgatg
     1441 ttcacgtctg aggtactggc catgctgaaa atcttttcgt tcgaggttct ccttcgccgt
     1501 aggctcgacg aacggggcgc gaatgtaggt gacccggacc tatcggcaat ggtcggccat
     1561 cggtatcgga aatgtgactt ggcctactgc acaatgacct tcctggtggc caccaactgg
     1621 ccgttcaact gccgggccag cagcggatag atgcccggtg caagacccga gacatcgatc
     1681 gccataggcg tgtcagccgt cgcgcgaacg cctacaatga cccttgcccc acgtgcatcg
     1741 tacagatcca gaaccgctgg gacacggcag gtcaccatca ctggcgagcc attcgaagca
     1801 ggattgttgg cgagcacgat cgcgctcacg tgctcggcct cgctgacgcc atccggtgct
     1861 tcgctgatca cggtgcaggc atcggtgaag gtccaaagag ggcggtcaac ggccgtgatg
     1921 gacatggttt gtacagtggt cgggatcggc tcggatgttc cggtcaatac ctcatcggcc
     1981 agcagtgact ccgggacctg gtcgtggcta acgcccaacg ggtaagaagc aatggcgcag
     2041 cccgaagtcg gatcgaagag gaacgaccag tccgctggcc gataggtggt gtaaccgaac
     2101 gtcaggtcag tcgagttgag atttccaatg acgtgtacgg tcccattctc gtacttgacc
     2161 ttagttgctg ccagggcatg cgtggcgttc atcccggtgg cccccaccgc acccgcaaga
     2221 tcgaggaagt cgaacgtcgg gctcatacgg gtaacacttg accccatcat catgagaagc
     2281 gtgtcgccga cctggccgat cgcggggccc atccatgttt ccccatacag atcggcacgt
     2341 tgccatgcgc ccgcaggcgt cagcacgaaa tggaatcctt tggagtcagc ccccaccatc
     2401 gcgttgccga tgatgcatag atccccgttg gacatcaggg cacctccctg ggcctggacg
     2461 tcgaaaatgt tgttctccaa ccagatgtcc ttggcccata ccaggtcacc gaccgcgctg
     2521 agacgggcca ccttggtcac ggaccccata aatgtattgt aactcaagta tactccaccg
     2581 atgccatccg gcacgaggat gtaactggtg ttattgagtg gtgagtccat aacgggcact
     2641 ctcctcgccc agatcaccgc tccgtccaag ccgatccgca gaaccatgac ctgcgtatcg
     2701 gatgaggaac tgaaactgcc gatcacgaac aggtctcccg ttcccgattg tacggcatcg
     2761 tacgtcggtg cggtcggcat ccaagccatg ctcgtgatcg aggacaagat tcgatgggac
     2821 caaagaggct cacctgtgct gttcagtgat gtcatgcgca gtgacagggt cgccatcaca
     2881 gtatcgaggt caaggggttc ccaataactc gaatcgttga acagatggag gtggccgccc
     2941 atcccattgg aaaagatctg tccataggca ttgctgggca ggccgatatc cttacgccat
     3001 accagtgtgc catccgcggc ggtctttacc aggtccgtgc catgtgatgt acgcaaggcc
     3061 acgacagcgc caccatcatc ggagaatccc gcatagcgca cggtcggatg aaggtccgtg
     3121 tccagtgtat cccatatcac ctgatgcagt tgctgggcgt tcgccgtaac agaggccacg
     3181 acaacgaaca cgaggccgta tgttgctttc atgatgtggc gtttgtggga tgcgtgactt
     3241 gctcgatcaa tgacgttgtg aaggtagcct tgcgctgcct cgtgccgcac atttcgtcac
     3301 tgtcccacct ctaccgaccg gtcacatgcg aaggtttcct ccgcctgccg gttgctggcc
     3361 accaccaggg tccgagcatc gaggttcttc gtcagcaagt cgcggaacca cgcgctcccg
     3421 tccttgtcca gattgctcgt gggctcgtcc aacaacagca aggatgtatc gctcagcacg
     3481 gcgagtgcca gcttcaaccg ctgcttcatc ccgctgctga ggtcgcgaac gggctttccc
     3541 atgtgtttgt ccagcagggc gatccgcgcc acctcttccg ccgtgagcgc accacgcacg
     3601 ggcttgaacc gcttgtgcga tgtgatcgtt tcgcgcaagg acagttcctc gtacaaactc
     3661 atgtaaggcg cagcaatact gatgtggcgg tacaccaggt cggggtccac cgttgggccg
     3721 tcgtgctcat gcgtcacggt gcctcgggtg ggaatggaga taccgccgat cagttgcagc
     3781 aaggtgctct tgccgctgcc gttcggaccc aggagcgcgg tgcgcgatcc gggctcgaag
     3841 acatgggaca ggtcgcgaat gacgtgctcg cgaccgaagg actttgagac gttctggagc
     3901 gtgatccgca tgggctcttc gcgatgccgg gacaggcccc ggcatgacag acttcttatt
     3961 ccgccagccc gcgcatgatg cccttggggc tgttgttgat gaagtcgacg atgacgccgc
     4021 gttcatggct gcgcggcatg gtctgcagca cattctgcac cgcccggtcc acgttggtgt
     4081 tgcgcacgaa gatctcgcga taaatgtcct cgatgcgggc cacctgctcg tcgttgaagc
     4141 cccggcggcg caaacccacc acgttcacac cgatgtagct gagcggctcg cgcgcggcct
     4201 tcacgaaagg cggcacgttc ttgcgcacca gcgaagcgcc ggcgatgaag ctgtgcgccc
     4261 cgatgtggat gaactgctgc accgccacgt tgccctccag
//
LOCUS       DAOQOC010000094         4289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9122_length_4289_cov_3.695796, whole genome shotgun sequence.
ACCESSION   DAOQOC010000094 DAOQOC010000000
VERSION     DAOQOC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9122_length_4289_cov_3.695796"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PL106_02420"
     CDS             <1..944
                     /locus_tag="PL106_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA31951.1"
                     /translation="RRHRGDTEGSSRMRILVTGSNGLLGQKLVAALRNDPEVKLIASS
                     RGEDRTPDHGDYLYLSLDCTDEAAVHRAFDEAHPDVVIHTAAMTNVDACELDPAGCQL
                     QNVTAVQYLVNACANIDAHFIHLSTDFIFDGEHGPYREEDRPAPLSLYGRSKWDAERI
                     VIHSGLKMWAIARTIIVYGIAKGLSRGNVVLWAKNALEKGQPIKVVHDQWRMPTLAED
                     LADGCIRIAKQGATGIFNLCGPDGMSILELVQRVARFFDLDPSVITPVTSDSLGQPAK
                     RPPRTGFVIDKARRELGYAPHGFEEGLVILRKQLQAV"
     gene            1048..2025
                     /locus_tag="PL106_02425"
     CDS             1048..2025
                     /locus_tag="PL106_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNA31952.1"
                     /translation="MSYRKHSRRPWKEELKDILDVHASERRGIAVLLVLCAIAAGWVL
                     YEQRIAPDVVRDRADFEVVARENIAQGSSGSVDQRSDLQGIDAAVVLFNFDPNGLPME
                     QWTALGLSERQAAAILRYEERGGRFRTKADLARMRVVDPELFARWEPFILLPDSIEIS
                     AVGERSRFAEPGRWRDTSARHFPGSPERPERVVVEVNTADSSRLVQVRGIGPAFARSI
                     VRYRDRLGGFHSLDQLADVPILRDKPDAVAELKGRLLLDTLMVRRLPINTMSVEDLGR
                     HPCAGWKVAKALVAYRQHHGPFRTVADIKGCVLITDSVYRKLAPYLTLE"
     gene            2022..2555
                     /locus_tag="PL106_02430"
     CDS             2022..2555
                     /locus_tag="PL106_02430"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNA31953.1"
                     /translation="MNLEERLKAAIRAVPNFPKPGILFRDITPVLEDPQLGRDCIEGF
                     RDQLRNTTIDAIAGIESRGFLFGLPLALAMGVPFLTVRKKGKLPWRTVAFKYDLEYGS
                     AEVEMHVDSVRPGMRVLIHDDLLATGGTAMAAAELVRMQGGSVAGFSFLIELAFLRGA
                     EHLQPYNTGLVSLATYE"
     gene            2565..3710
                     /locus_tag="PL106_02435"
     CDS             2565..3710
                     /locus_tag="PL106_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA31954.1"
                     /translation="MQLAVSENQLMIAQAVRDLVEREVRPNLMEWDESQHFPKKLFTD
                     HFGPAGMLGVFVPEEYGGAGLGYHEYVDTIVEVARVCGSIGLSVAAHNSLCTGHINYF
                     GNHEQKKKWLTKLATGEWLGAWALTEPNTGSDAMRMKCTARKEGKEWVINGTKCWITH
                     GISSDVMVAIVRTGELLDSKGMTAFVIERGTPGLKAGKKENKLGMRASETAEVIFEDC
                     RIPEENVLGEVGQGFQQAMKILDGGRISIAALSLGIAKGALDASVKYAKERQQFGQPI
                     ANFQGISFKLADMATRVEASELLIRHASDLKNNNQKVTKAGAMAKLYASEVCVWASNE
                     AVQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVIGREVLKA"
     gene            complement(3759..4130)
                     /locus_tag="PL106_02440"
     CDS             complement(3759..4130)
                     /locus_tag="PL106_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA31955.1"
                     /translation="MNKKEIGMRMFALRTEHHVQQKEVAAVLGISPSAYCDRENGHVI
                     PSALEVVKLAEYYGVGLDEFFRGGQPVLNMQDHSSNGYNVIHSQTVNGLSDELMQRFI
                     DVMATNAAALEKLATVLSSKV"
BASE COUNT          865 a         1274 c         1340 g          810 t
ORIGIN      
        1 cgcgccgcca tcgtggggac acggaaggat cgagccgcat gcgcatcctg gtgaccggaa
       61 gcaatggcct gctcggccag aaactcgtcg ctgccttgcg caacgacccg gaggtcaaac
      121 tcatcgccag ctctcggggc gaggatcgca ccccggacca cggggactat ttgtacctat
      181 cgctcgattg cacggatgag gccgccgtgc atcgggcttt cgatgaggcc catcccgatg
      241 tggtgatcca taccgctgcg atgacgaacg tggatgcctg cgaactggat cccgcgggct
      301 gccagctcca gaatgtcact gccgtacagt acctggtgaa cgcatgcgcg aacatcgatg
      361 cgcacttcat tcatctcagc acggatttca tcttcgacgg agagcacggc ccgtaccgcg
      421 aggaggaccg gcccgcgccg ctgagcctct atgggcggag caagtgggac gctgaacgga
      481 tcgtgatcca cagcgggttg aagatgtggg ccatcgcacg aacgatcatc gtgtacggca
      541 tcgccaaggg cctcagccga ggcaatgtgg tgctgtgggc gaaaaacgcc ttggagaaag
      601 ggcagcccat caaggtggta cacgaccaat ggcgcatgcc cacattggcc gaggatctgg
      661 ccgatgggtg catccgcatc gcgaagcaag gcgctacagg gatcttcaac ctctgtggtc
      721 ccgatgggat gagcatcctt gaactcgtgc agcgggtcgc ccgcttcttc gatctggatc
      781 ccagcgtgat cacgccggtc acttccgaca gcctgggaca acccgcgaaa cggcccccga
      841 gaaccggttt cgtcatcgac aaggcccggc gcgaactcgg atatgcgccg cacgggttcg
      901 aggaaggcct ggtgatcctg cggaagcagt tgcaggccgt ataagctcac atccggttgt
      961 ggatgggtcg gtgggtggtg catggtgtac atgaccctgc gccggatcgc cacctgacgg
     1021 tatcttccgc accagaaaga tcccgacttg tcgtatcgca agcattctcg gcgcccttgg
     1081 aaggaggagt tgaaggacat cctcgatgtc catgcgtccg aacgacgcgg tatagcggtg
     1141 ctgctcgtgc tttgtgcgat cgccgccggg tgggtgctct acgaacaacg catcgccccg
     1201 gatgtggtac gcgatcgtgc ggacttcgaa gtggtcgccc gggagaacat cgcacaggga
     1261 tcttccggat ccgttgatca acgatccgat cttcagggta ttgatgcggc ggtggtcctt
     1321 ttcaacttcg atcccaatgg acttcccatg gaacagtgga ccgcattggg gctgagtgaa
     1381 cgacaggccg cggcgatcct ccggtatgag gagcggggcg gccgcttccg caccaaggcc
     1441 gaccttgccc gtatgcgagt ggtggacccc gaactcttcg ctcgttggga gccgttcatt
     1501 ctcctgcccg actccatcga gatctccgct gtaggagagc gctcgcgctt tgcggagcct
     1561 ggacgttggc gggatacttc cgcccggcat ttccccggat cccccgaacg cccggagcga
     1621 gtggtggtgg aggtgaacac cgcggacagc agccgcttgg tgcaggtgcg cggcatcggc
     1681 ccggcattcg cccgctccat cgtgcgctat cgcgaccgcc tgggcggttt tcacagcctc
     1741 gaccaactgg ctgatgtgcc catcctgcgc gacaagcccg atgccgtcgc ggaattgaaa
     1801 gggcgcttgc tcctggatac gttgatggtg cgccggctgc ctatcaatac catgtccgtc
     1861 gaagacctgg gacgacaccc ctgtgctgga tggaaagtgg ccaaggcact ggtggcttat
     1921 cgtcagcacc atggaccctt ccgcaccgtt gcggatatca agggctgcgt cctgataacc
     1981 gacagtgtct accgtaaact cgcaccttat ctcacgctgg agtgaatctc gaagaacgcc
     2041 tcaaggccgc cattcgtgca gtgccgaact ttcccaagcc cgggatcctc tttcgcgaca
     2101 tcacccccgt gttggaggat ccgcaactcg gtcgggactg tatcgaaggc ttccgtgatc
     2161 agttgaggaa tacgaccatc gacgcgatcg ccggtatcga aagccgtggt ttcctgttcg
     2221 ggttgccctt ggcgctggcg atgggcgttc cgttcctcac cgtgcggaaa aagggcaaac
     2281 tcccgtggag gaccgtggcg ttcaagtacg accttgaata cggcagcgcc gaagtggaaa
     2341 tgcacgtgga cagcgtgcgg cccggtatgc gggtactcat acacgacgac ctcctggcca
     2401 cgggaggtac cgccatggcc gccgcagagc ttgttcgcat gcagggaggt tcagtcgccg
     2461 gtttcagttt tctcatcgaa ctggcgtttc ttcgcggcgc agaacacctg cagccatata
     2521 acaccgggct ggtgagcctg gccacctacg aatgatccct gcccatgcaa ctcgctgtat
     2581 ctgagaacca gcttatgatt gcgcaggccg tgcgcgacct cgtggaacgt gaagtgcgcc
     2641 cgaacctgat ggaatgggat gaatcgcagc actttcccaa gaaactcttc accgatcact
     2701 tcggcccggc cggtatgctg ggtgtgttcg tgccggagga atacggtggt gccggcctcg
     2761 gataccacga gtatgtggac accatcgtgg aggtggcacg tgtgtgcggg agcatcggcc
     2821 tgagcgtggc cgcccacaac tcgctctgca cgggccacat caactacttc ggcaaccacg
     2881 agcagaagaa gaagtggctc accaagctcg ccaccggcga atggctgggc gcatgggcac
     2941 tcaccgaacc caataccggc agcgacgcca tgcgcatgaa gtgtaccgcc cgcaaggaag
     3001 ggaaggagtg ggtcatcaac ggaaccaagt gctggatcac ccacggcatc agcagtgatg
     3061 tgatggtggc catcgtacgc accggggaac tcctcgacag caaaggcatg acggccttcg
     3121 tgatcgaacg tggcactccg ggactgaaag cgggtaaaaa ggagaataag ctgggcatgc
     3181 gcgccagtga gaccgccgag gtgatctttg aggattgccg catccccgag gagaacgtcc
     3241 tgggcgaggt ggggcagggc ttccaacagg ccatgaagat cctggacggt ggtcgcatca
     3301 gcatcgccgc gctcagcctg ggcatcgcca aaggtgcatt ggacgccagc gtgaagtacg
     3361 ccaaggaacg ccaacagttc ggccagccca tcgccaactt ccaagggatc agcttcaagc
     3421 tggccgacat ggccactcgg gtcgaggcca gcgaactgct catccggcac gccagcgacc
     3481 tgaagaacaa caaccagaag gtgaccaagg ccggggccat ggccaagctc tacgccagcg
     3541 aggtgtgcgt atgggccagc aacgaggccg tgcagatctt cggcggctac ggctacacca
     3601 aggatttccc ggtggagaaa ttctaccgcg acagcaagct ctgcaccatc ggcgaaggaa
     3661 ccagcgagat ccagaagctg gtgatcggca gggaagtgct caaggcatag gccatgtgag
     3721 gagcgcgtgc ggatcggcgc agttgtgctc gggtgtgact ataccttgga ggaaagcacc
     3781 gtggccagct tttccaaggc tgctgcgttg gtggccatca cgtcgatgaa ccgttgcatc
     3841 agctcatcgc tcaggccgtt cactgtttgc gaatggatga cgttgtatcc gttcgaggag
     3901 tggtcctgca tgttgagcac gggctgccct cctcgaaaga attcgtccag gccaacgcca
     3961 taatattctg ccagtttgac cacctccaag gccgagggga tcacatggcc gttctcgcga
     4021 tcgcaatagg ccgatgggct gatcccgagt acggcggcca cttctttctg ctgcacgtgg
     4081 tgttctgttc ggagtgcgaa catgcgcatg ccgatctcct tcttgttcat cgcggtggtc
     4141 ttcggaggcg aatttatccg gctgggccga tatcgacttc gccctggccg aattcgggtc
     4201 cggtgaggag aaaggcattc ctgcaccgga tagtttcggt tcatggcggg agagacaacc
     4261 cgcgccatgc ccacacgaac cagcgcaac
//
LOCUS       DAOQOC010000095         4289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9123_length_4289_cov_3.325697, whole genome shotgun sequence.
ACCESSION   DAOQOC010000095 DAOQOC010000000
VERSION     DAOQOC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9123_length_4289_cov_3.325697"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PL106_02445"
     CDS             <1..367
                     /locus_tag="PL106_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31956.1"
                     /translation="HGTHITVAHAMGSTIGINTTILFASVFHIGGAALTRPARTGFWI
                     FNGSLLVFWLCLIGAGLVKGWLTVTTDMVFQSIMEEARPFITGFAISGMGLFIGLTLL
                     AVHAGKALLGGGTGQVENH"
     gene            373..1335
                     /gene="nadA"
                     /locus_tag="PL106_02450"
     CDS             373..1335
                     /gene="nadA"
                     /locus_tag="PL106_02450"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNA31957.1"
                     /translation="MILDDPALDGISEYGLREALLALRTERNAVFLAHYYQTPDIQEL
                     ADFVGDSLALAQAADRTGADTILFCGVHFMAETAKILNPGRTVLLPDLDAGCSLADSA
                     PADKFRAFLDAHPGHTVVSYINCSAAVKAMSDIICTSSNAVRVVNSLPKDRPVIFAPD
                     KHLGAYVQRMTGRRLVLWDGVCEVHVDISADKLKLLLGKHPEAKLVAHPECPEHILAE
                     ADHVGSTSSLLDFVQRDPASTFIVATEAGILHRMREAVPHKVLIPAPAHANNTCACSE
                     CPYMKRNTVRKMYTALRDGRPAIELPEDVRRGAERSLRRMLALG"
     gene            1477..3003
                     /gene="nadB"
                     /locus_tag="PL106_02455"
     CDS             1477..3003
                     /gene="nadB"
                     /locus_tag="PL106_02455"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNA31958.1"
                     /translation="MNSPLDVIVVGGGIAGMACALELAQQQGTRKVRVRIIAKEPVIG
                     SNSYAAQGGMAAVLDPADSMAAHVRDTLTVGEGRNDLRVVRRVVREGPAMVHGLLAMG
                     AVFDRNADGGVALAREGGHTTARVVHRLDRTGAEIVRVLHQHVRASSSISVIDEQAVD
                     LLVDGGACIGVRTRDPRTGRWMDRIAHAVVLATGGTGQVYEHTTNPPGATGDGIAMAV
                     RAGVPLKDMAFVQFHPTALYTDRPGTVFLISEAVRGAGARLLRPDGRPLMEGVHPQGD
                     LAPRNVVARTIARVMLRGHVPNVWLDTSPIGIRRFARDFPSINTHCRELGIMPGRDRI
                     PVMPAAHYLCGGIMTDAQGRTTMKGLYALGECACSGLHGADRLASNSLLEALVIPRHA
                     AARILRARTKHATLTDHHSPDVPVADTVANDDHEAMLHGLRRTMTRHAGILRTTNGLH
                     LALRRIEQAGLRTERAWAKGERAPGLIALRNAVAVSRSIVMAALSEPVSTGTHWIGRM
                     "
     gene            complement(3092..>4289)
                     /locus_tag="PL106_02460"
     CDS             complement(3092..>4289)
                     /locus_tag="PL106_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNA31959.1"
                     /translation="PMGLLGDRWALLGHQGWEFVEFGRAFQALLMGILVLWGVIVYRG
                     VRPVLHKGRPWELPNWLIYSIVCVTLLLISGFVAGPRTNFVIADFWRWAVIHMWVEAF
                     FEVFTTVVVGFLMVRMGLVGRQSVTRVVYLAAILFLGSGLLGISHNFYWNAKPVATMA
                     LGSVFSTLQVIPLVLLTLEAWRFSRMSRHGSLRHSPNGGRAFAFGHTEVFLFLVAVNF
                     WNFMGAGVFGLIINLPIVNYYEHGTYLTVNHGHAALFGVYGNLSLAGLLFCCHTLLRP
                     EKWNGRLVRRAFWSLNIGLSLMVVMDLLPAGLVQLGAVLDRGYWFARSSGFVEVSAFH
                     WLTWLRGVGATLFILGGVLPIAWMVLQGAGRLKPGKSRANEPVLEDRPVGHDPHGSKV
                     VMDPSM"
BASE COUNT          831 a         1363 c         1394 g          701 t
ORIGIN      
        1 ccacggcacg cacatcaccg tggcccatgc catgggcagc accatcggca tcaacaccac
       61 catcctcttc gcctccgttt tccacatcgg cggcgcggca cttacgcgac cggcacgcac
      121 gggcttctgg atcttcaacg ggtctttgct cgtcttctgg ctctgcctga tcggtgcggg
      181 cctggtgaaa ggctggctca cggtgacaac ggacatggtg ttccagtcga tcatggagga
      241 ggcccggccg ttcatcacag gcttcgccat ttcgggcatg ggtctgttca ttggccttac
      301 gttgctggcg gtgcatgcgg ggaaggccct gctgggtggc ggaacgggac aagttgaaaa
      361 ccattgatgc ccatgatcct cgatgatccc gcattggacg gcatttctga atacggcttg
      421 cgcgaagcct tgctcgcgtt gagaaccgaa cggaacgcgg tcttccttgc acattactac
      481 cagaccccgg acatccagga actggccgac ttcgtggggg acagcctcgc attggcacag
      541 gccgctgacc ggaccggggc ggacaccata ctcttctgcg gtgtgcattt catggccgaa
      601 acggccaaga tcctgaatcc gggccgcacc gtgctgctgc ccgacctcga tgcgggatgc
      661 tcgctcgccg acagtgcccc cgccgacaag ttccgcgcgt tcctcgatgc gcatcccggc
      721 cacacggtgg tgagctacat caactgcagc gcggcggtga aggccatgag cgacatcatc
      781 tgcaccagca gcaatgcggt ccgcgtggtg aacagcctgc ccaaggaccg ccctgtgatc
      841 ttcgcgccgg acaagcatct gggagcgtac gtgcagcgca tgaccggtcg tcgattggtg
      901 ctgtgggatg gcgtgtgcga ggtccacgtg gacatcagtg ccgacaagct gaaactgttg
      961 ctcggcaagc atcccgaagc gaaactggtg gcgcatccgg aatgcccgga gcatatcctg
     1021 gcagaagccg accatgtggg gtccacctca tcattgctgg acttcgtgca gcgcgatccg
     1081 gcgagcactt tcatcgtggc caccgaggcc gggatcctcc atcggatgcg cgaggccgtg
     1141 ccgcacaagg tgctgatacc cgcacccgca catgccaaca acacctgcgc gtgcagtgag
     1201 tgcccctata tgaagcgcaa cacggtgcgc aagatgtaca ccgcactgcg cgatggacga
     1261 cccgccatcg aattgccgga ggatgtaagg cgcggtgctg aacgttcgtt gcgtcgcatg
     1321 ctcgcactgg ggtgacggga tgagcggaca tgatccacat tcaaatatgt atactggtgc
     1381 atatattgtt ttgaaggcgc tttcccgccc tcgcgatcca ctgcccgttc cgcggaggcc
     1441 atcctcccgc gaccttgtac ccgcacccca cgcagcatga acagccccct tgacgtgatc
     1501 gtggtgggcg gtggcatcgc cgggatggcc tgtgcgctgg agctggcaca acagcagggg
     1561 acccgcaagg tccgggtgcg catcatcgcg aaggaacccg ttatcggtag caattcgtac
     1621 gcggcacagg ggggcatggc ggcggtactg gatcctgcgg attcgatggc ggcgcacgtg
     1681 cgggacacgc tcacggtggg cgaaggccgg aacgacctgc gggtggtgcg gcgtgtggtg
     1741 cgggaaggtc cggccatggt gcatgggctg ctggccatgg gtgcggtatt cgatcggaac
     1801 gcggatggtg gtgtcgcgct ggcccgtgag ggtgggcata ccacggcgcg ggtggtgcat
     1861 cgcctggacc gcaccggtgc cgagatcgtt cgggtgttgc accagcatgt gcgcgcatcg
     1921 agttcgatca gcgtgatcga tgagcaggcc gttgacctcc tggtggacgg tggggcctgc
     1981 atcggtgtgc gcacacggga cccacgcacc ggtcgatgga tggaccgcat cgcgcatgcc
     2041 gtggtactgg ccaccggcgg tacgggacag gtgtacgagc acaccaccaa tccgccgggc
     2101 gcaacgggcg atgggatcgc catggccgtc cgcgcgggcg tacccctgaa ggacatggcc
     2161 ttcgtccagt tccatcccac ggccttgtac acggatcgcc caggcaccgt cttcttgatc
     2221 agcgaggccg tgcgcggtgc aggtgccagg ctcttgcgcc ccgatggccg cccgttgatg
     2281 gagggcgtgc atccgcaagg cgatctcgcc ccgcgcaatg tggtggcccg cacgatcgcg
     2341 cgggtgatgt tgcgcggaca tgtgccgaac gtttggttgg acacctcgcc gatcggcatc
     2401 cggcgcttcg cccgcgattt cccgtcgatc aacacgcact gccgcgaact ggggatcatg
     2461 cctgggcgcg acaggatccc cgtgatgccc gcggcgcatt acctgtgtgg aggtatcatg
     2521 acggatgcgc aggggcggac cacgatgaag ggcctgtacg cactgggcga atgcgcgtgc
     2581 agcgggttgc acggagccga ccgcctggca tccaactcgc tcctcgaagc actggtgatc
     2641 ccccggcacg cggccgcccg catccttcgc gcacggacga agcatgccac gctgacggat
     2701 catcacagcc ctgacgtgcc agttgccgat acggtcgcga atgatgacca tgaggccatg
     2761 ctccacgggt tgcgccgcac gatgacgcga catgccggca tcctccggac caccaatggc
     2821 ctgcacctcg ccctgcgacg gatcgaacag gcgggcttgc gcacggagcg cgcttgggcg
     2881 aagggggaac gcgcacccgg gctgatcgcg ttgcgcaacg ccgtggccgt atcgcgcagc
     2941 atcgtgatgg ccgcgttgag cgagccggtg agcacgggca cacattggat cggccggatg
     3001 tgagacacga cctccgccat acggcgtggc ggtgatacgg cgcacggatg cgatgcgcaa
     3061 catccggtac cccgggaaca aaaggagcgc gttacatgga gggatccatc accaccttgg
     3121 atccgtgcgg atcgtgcccg accggtcgat cctccagcac cggttcgttg gccctgctct
     3181 ttccgggctt caatcgccca gcgccctgga gcaccatcca cgcgatgggg agcactccgc
     3241 ccagaatgaa cagcgtagca ccaacaccgc gcagccaggt cagccaatgg aaagccgaga
     3301 cctccacgaa accgctggag cgcgcgaacc aatagccgcg atcgagcacc gcgcccagct
     3361 gcacgaggcc ggcgggcagc aggtccatca ccaccatcag cgaaaggccg atgttcagcg
     3421 accagaacgc gcggcgaacg agccgcccgt tccacttctc cggcctgagc agggtatggc
     3481 agcagaacag gagtccggcc agggagaggt tgccgtaaac accgaacaac gcggcatgcc
     3541 catggttcac cgtcaggtac gtaccatgtt cgtagtaatt tactatgggc aggttgatga
     3601 tcagaccgaa cacaccggca cccatgaaat tccagaagtt caccgctacc aggaaaagga
     3661 agacctcggt atgcccgaaa gcgaaggcgc gaccgccatt gggcgagtgg cgcagcgagc
     3721 cgtgccggct catgcgggag aatcgccagg cctccagcgt gagcaatacg agcgggatca
     3781 cctgcagggt ggagaacacg ctgccgagcg ccatggtggc cacgggtttc gcattccagt
     3841 agaaattgtg cgagatgccc agcagcccgg agcccaggaa caggatggcg gccaggtaca
     3901 ccacgcgggt gaccgactgt cggccaacca gccccatgcg taccatgagg aagccgacca
     3961 ccacggtggt gaagacctcg aagaaagcct ccacccacat gtggatcacc gcccagcgcc
     4021 agaagtcggc gatgacgaaa ttggtgcgcg ggccagcgac gaagcccgag atcagcagaa
     4081 gggtgacgca cacgatggag tagatcagcc aattgggcag ttcccagggc ctgcccttat
     4141 ggagcacggg gcggacgccg cggtacacga tcacgcccca gagcaccagg atgcccatca
     4201 gcaacgcttg gaacgcgcgg ccgaactcca cgaactccca gccctggtgg ccgagcaagg
     4261 cccagcgatc accgagcagg cccatgggc
//
LOCUS       DAOQOC010000096         4287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9135_length_4287_cov_3.098062, whole genome shotgun sequence.
ACCESSION   DAOQOC010000096 DAOQOC010000000
VERSION     DAOQOC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9135_length_4287_cov_3.098062"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PL106_02465"
     CDS             <1..259
                     /locus_tag="PL106_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31960.1"
                     /translation="EALTTAALKKKIKSLVDAKTGTKALQRIHDLLTYPALDDRQKRA
                     LTERLDLAEEDFKAGRVMGAEEARTRLRASLKAYRVTPAGG"
     gene            374..598
                     /locus_tag="PL106_02470"
     CDS             374..598
                     /locus_tag="PL106_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31961.1"
                     /translation="METQALKNDLIAWIQRLQDRGILESLAMLKRGAEAHDWADDLSP
                     EARASIELGLDDIKQGRTMSSEEFWKKRGR"
     gene            588..881
                     /locus_tag="PL106_02475"
     CDS             588..881
                     /locus_tag="PL106_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA31962.1"
                     /translation="MAGELSVRWSAQADRAAGEMAAFIASQWTEKEVKHFLDLLEEFE
                     RRVVRFPFSFPASEGYAGCRRASIHRNASVVYRVDEGIIHIVTVVDNRSSKTR"
     gene            1044..1886
                     /locus_tag="PL106_02480"
     CDS             1044..1886
                     /locus_tag="PL106_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31963.1"
                     /translation="MKRALFLLLTLAPVLVIAQGRYGRKRPDIIPLDGQARRIGWYVA
                     PGVTYTLSRFKDREEEVFRAGDTSLVATYNPDGRPGVYLEAGLAWLTRDPVIVDYLDL
                     GIAYKNLRGVESFTGRYTRADSSMDLAGTGAFAERMITLHFNANRFIHTRPYQFVQLS
                     LGANADYRLGSSYEYTGAPSFTGWSFPPAWWAQLHFKVGYGFKLTGQMILIPAIETPV
                     FSVLPGDEGRFGALQWFSSDYRPLILSVRFLFLRARKGWECPPVKNNEFERHKVVNPE
                     YKRQ"
     gene            complement(1931..2563)
                     /locus_tag="PL106_02485"
     CDS             complement(1931..2563)
                     /locus_tag="PL106_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA31964.1"
                     /translation="MRITRFTFNPFDENTWVLDNGREALLVDPGCSDADEQRELENWL
                     ATNRLTPIRLLLTHAHLDHVLGCAWMHRRFGLLPELHRADLPLLELAERQGLMYGIPC
                     EPPPAPTKFLAPGDTITLGNERLEVLFVPGHAPGHIAFWHPEQRFVISGDVLFQRSIG
                     RTDLPGGDMDTLIASIRTELFPLGDDVKVHCGHGPDTTIGEERRLNPFLR"
     gene            2593..4152
                     /locus_tag="PL106_02490"
     CDS             2593..4152
                     /locus_tag="PL106_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31965.1"
                     /translation="MKVRTGMPAQGPDGAIVRGWADREVAHQGTRQGVAKDRLSRLVL
                     LVLLSFVLAPARAQFYNGSMQEYGKNRVQYQDFLWQYYRFQDIETYFYKGGRDVARYV
                     SISGHRNKREMERFFDYTLDDRIQFLVYNSLGDFRQSNVGLGVDDRYNIGGVARIVGT
                     KVFVYNEGDHELLERQVRSGIAEMMIDQMMYGGNWRQVIKNSTLLNLPEWFTKGLVAY
                     CAGPWDARSAGRIRDGVLGTRFKRFNRLEGDDAALAGLIVWTYVAETYGVSVIPNILY
                     MTRVSRNPENGFKYVLGTGLQQLTQECMDHYRAKFMEEDRLRADRDLEELKVRTRKAR
                     TYAEFKLSPDGRYAAWTSNESGQYKVWLHDRTTGKTRRIAKGEKKLDRIIDRSWPVLA
                     WHPTSRALTWTTEKKGELYLTTYTLDDRSRTRKPVFMLEKILSMAYGSDGQTMVFSGV
                     LEGRTDLYLYHVIGNRQDKLTDDQYDDLDPVFTADGTGVLFSSDRPDDTLRANADVRQ
                     AYLGEQEAVHG"
BASE COUNT          860 a         1270 c         1385 g          772 t
ORIGIN      
        1 ggaagccttg accacagccg cactgaagaa gaagatcaag tcgttggtgg acgccaagac
       61 gggcaccaaa gcccttcaac gcatccatga cctgcttacc tatccggcct tggacgacag
      121 gcagaagcgg gcattgacgg agcggctgga ccttgccgaa gaggatttca aagcgggtcg
      181 tgtgatgggc gcggaagaag cccgcacgcg gttgcgggct tcgctgaaag cgtatcgcgt
      241 aacccccgct ggaggatagg ctcagaaccg tgtccttcat cgtccgcttc agcctggatc
      301 caccgagcga caacaagctc acggtgctca ggtgcgtgaa acactacctt tgaggacacc
      361 aacgacctcc agtatggaaa cgcaagcgct taagaacgac ctcatagcgt ggatccagcg
      421 gttgcaggat cgggggatcc ttgagtcgct ggccatgttg aaacgaggtg ccgaagccca
      481 cgattgggcg gacgacctct cccccgaggc gcgggcctcg atcgagcttg gcttggatga
      541 tatcaagcag ggccgcacga tgagcagcga ggaattctgg aagaagcgtg gccggtgaac
      601 tgagcgtacg gtggtcggca caggccgatc gggccgccgg tgaaatggcc gcattcatcg
      661 cgtcgcagtg gacggagaag gaggtgaagc attttctcga tctcctcgaa gagtttgagc
      721 gacgcgtggt gcgcttcccc ttcagtttcc cggcctcgga aggctatgcg gggtgccgcc
      781 gggcctccat ccatcggaac gcatcagtgg tctaccgggt ggatgaaggc atcatccaca
      841 tcgttaccgt ggtggacaac cgttcgtcga agacgcgata gccgagcgtt gatcggttca
      901 cacctcctca gggtctccgc aaccgtgatg tgcgttcgag agggtcggga ccctgagggg
      961 atccatccac cctgttgatc accgcgttga cactttctgg cgcagcgtct gcgttctgca
     1021 tgcacgcacc tgaatttcgc ggtatgaagc gagccttgtt ccttcttctc acgctggctc
     1081 ccgtgctggt gatcgcccaa ggccgctacg gccgcaagcg cccggacatc atcccgttgg
     1141 atggacaggc gcggcgcatc ggttggtacg tggcgccggg tgtcacctac accttatcgc
     1201 gtttcaagga cagggaagaa gaagtgttcc gcgcgggcga tacgagcctc gtggccacct
     1261 acaacccgga tggacgacct ggcgtgtacc tggaagccgg cctggcttgg ctcacccgcg
     1321 atcctgtgat cgtggactac ctggatctgg gaatcgcata caagaacctg cgcggggtcg
     1381 agtcgttcac cggccgatat acccgtgcgg acagcagcat ggacctggcc gggacgggtg
     1441 ccttcgcgga gcgcatgatc accctgcact tcaacgccaa ccgcttcatc cacacgcggc
     1501 cgtaccagtt cgtgcaactt tccctggggg cgaacgccga ctaccgcttg ggcagcagct
     1561 acgagtacac cggggcccca tcgttcaccg gatggtcgtt cccgcccgct tggtgggccc
     1621 agttgcattt caaggtggga tatgggttca agctcaccgg acaaatgatc ctcatccctg
     1681 ccatcgaaac gcccgtcttc agcgtgctgc ccggtgacga gggccgcttc ggcgcgttgc
     1741 agtggttcag cagcgactat cgtccgctca tcctcagcgt gcgcttcctc ttcctgcgtg
     1801 cgcgcaaggg ctgggaatgc ccgccggtga agaacaacga attcgagcgg cacaaggtgg
     1861 tgaaccccga gtacaagcgg cagtagcttt tccgctttgc gcaggcccgt gccggttcgt
     1921 gcaatggaac ctaccgcaaa aaagggttca gccgccgttc ctcgccgatc gtggtgtcgg
     1981 ggccgtgccc acagtgtacc ttcacatcat cacccagcgg gaagagttca gtgcggatgc
     2041 tcgcgatcaa ggtatccatg tcgcctccgg gcaggtctgt ccggccgata ctgcgctgga
     2101 agaggacatc accactgatc acgaagcgct gctcgggatg ccagaacgcg atgtgaccag
     2161 gagcgtggcc cggtacgaag agcacctcca gtcgttcatt gcccaaggtg atggtgtccc
     2221 cgggtgcgag gaacttcgtt ggtgcgggtg gcggttcgca cgggatgccg tacatcaggc
     2281 cctgtcgttc ggccagttcc aacaacggaa ggtcagcgcg gtggagttcg ggcaacaggc
     2341 cgaagcggcg gtgcatccac gcacaaccca acacgtggtc caggtgggca tgggtgagca
     2401 gcagccgtat cggggtcaac cggttggtcg ccaaccaatt ttccagttcg cgctgttcgt
     2461 cggcatccga acagcccggg tccaccagca gagcctctcg gccgttgtcc agtacccagg
     2521 tgttctcatc gaacggattg aaggtgaaac gggtgatgcg caaaacggcc ggaaatgagt
     2581 gaacttcgcg cggtgaaggt acggacaggc atgcccgcac aagggcccga tggtgcgatc
     2641 gtgcgagggt gggcggaccg ggaggtcgcg catcagggca cacgacaagg cgtggcgaag
     2701 gatcgccttt cccgattggt cctgctcgtc ctgctcagct tcgtgctggc cccggcgcgg
     2761 gcgcagttct ataacggcag catgcaggag tacggcaaga accgtgtgca gtaccaggac
     2821 ttcctgtggc aatactaccg gttccaagat atagagacgt acttctacaa gggtggccgc
     2881 gatgtggcgc gatacgtgtc catcagcggg caccgcaaca agcgggagat ggagcgcttc
     2941 ttcgactaca cgctcgacga ccgcatccag ttcctggtgt acaactcgct gggtgatttc
     3001 cgccagagca acgtgggtct cggggtggac gaccggtaca atatcggggg tgtggcgcgc
     3061 atcgtgggca ccaaggtctt cgtgtacaac gagggcgatc acgaactgct ggaacggcag
     3121 gtgcgcagcg gcatcgcgga gatgatgatc gaccagatga tgtacggcgg caactggcgc
     3181 caggtgatca agaacagcac cctgctcaac ctgcccgagt ggttcaccaa ggggttggtg
     3241 gcatactgcg ccgggccgtg ggacgcccgg agcgcggggc gcatccgcga cggtgtgctc
     3301 ggcacgcggt tcaaacgttt caaccggttg gaaggggatg atgccgcgct cgcgggcttg
     3361 atcgtgtgga cctacgtggc cgagacctat ggggtgagtg tgatccccaa catcctgtac
     3421 atgacccgcg tgagccgcaa ccccgagaac ggcttcaaat acgtgctggg caccggcctc
     3481 cagcagttga cgcaggagtg catggaccac taccgcgcca agttcatgga agaggaccgt
     3541 ctgcgtgcgg accgcgacct ggaggaattg aaggtgcgta cacgcaaggc acgcacgtac
     3601 gcggagttca aactgagccc cgacgggcgc tacgcggcgt ggacgagcaa cgaatcgggc
     3661 cagtacaagg tgtggctgca cgaccgtacc acgggcaaga cccgccgcat cgccaaaggg
     3721 gagaagaagc tggaccggat cattgaccgg agctggccgg tgctggcctg gcacccgacg
     3781 agccgtgcgc tcacctggac caccgagaag aaaggcgagc tctacctcac cacgtacacg
     3841 ctcgacgacc gttcgcgcac ccgcaaaccg gtattcatgc tggaaaagat cctcagcatg
     3901 gcatacggtt cggacgggca gaccatggtg ttcagcggtg tgctcgaggg gcgtaccgac
     3961 ctctacttgt accacgttat cgggaaccgg caggacaagc tgacggatga ccagtacgac
     4021 gacctggatc ccgtgttcac cgccgatggt actggcgtcc tcttcagcag cgaccggccg
     4081 gacgacacct tgcgtgccaa cgcggatgtg cggcaagcct atctcggcga gcaggaggcc
     4141 gtgcatggct gaacccttgc ttgagatcca aggcatcgag acctgctacg gccgcagcca
     4201 ggtgctgttc ggcttgtcgc tctcgatcgc caagggcgaa atggtgacgt tgatgggtcg
     4261 caacggcatg ggcaagacca cgaccgt
//
LOCUS       DAOQOC010000097         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9151_length_4283_cov_2.941816, whole genome shotgun sequence.
ACCESSION   DAOQOC010000097 DAOQOC010000000
VERSION     DAOQOC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9151_length_4283_cov_2.941816"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PL106_02495"
     CDS             complement(<1..716)
                     /locus_tag="PL106_02495"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31966.1"
                     /translation="MRVSLLIIIGALAFPVHAQLGRRQKSFSSADTLRGSIGTERAWW
                     DVLHYDVSVQPDLATKSIRGTTIIGFIAKDNGLRMQIDLQQPLVVDSVTADVSRFRRG
                     SFTIEDERAHTEREGNVLWVDLPQPLKAGEASTIRITYHGVPREAKQPPWDGGWIWTY
                     DAQGNPWMSVACQGLGASVWYPCKDTQSDEPDDGASLHITVADSLQAVGNGRLRDVTR
                     NADGTSTWNCSSCTPRTRRR"
     gene            complement(788..1888)
                     /locus_tag="PL106_02500"
     CDS             complement(788..1888)
                     /locus_tag="PL106_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HNA31967.1"
                     /translation="MRTGRTTIGNVGRLLITVSLLLCIRSDSNAQFGAFVRKLLVGDT
                     LAPPNYDTAYISTFRQRLTLSLVSSYRLATLDISDTLGHAVSWSTNNATQYGVALDSR
                     WLSVEATFSIPALDPADPLYGRTSSKGVALGYTGRHIWVRGFWNYNSGFYADPPNAIA
                     PDHIDGQPWPHRPDLGVETWMASVNYALNRKNRYSQVAALTQMERQKRSAGTFVCGAA
                     FWLTRMNADSALVPTTGPLAFAPEAGIRASRRTIVGTTVGYAHTFVFWHKGFIHLALL
                     AGAGYSDQTRTMGKNGERAADTGVSALMEFKGGIGFNGDRWYTGLTLAYYLNEDADDH
                     VAMLGSMYGSARWAIGYRLGRPNIKGLDKVGL"
     gene            complement(1925..2452)
                     /locus_tag="PL106_02505"
     CDS             complement(1925..2452)
                     /locus_tag="PL106_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNA31968.1"
                     /translation="MPTPIPPPPPALFPTLQEEYNTCLFHAATALGRSLTALAEEQFG
                     PLELTPTMAFILMTTRTAPGILSTDLAHVHQLDVSTISRAVDKLVHERFVQREGTRKN
                     IRIFITPLGEKKEADARAAWAKLRQAYGLVLTDPGARDLADHIARSDGRLREARPRKR
                     RTANGKNIAVPGTGA"
     gene            2722..3270
                     /locus_tag="PL106_02510"
     CDS             2722..3270
                     /locus_tag="PL106_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNA31969.1"
                     /translation="MPTRIRLQRKGKKGQPYYHVVVADQRAPRDGKYIERIGAYEPGR
                     NPAFIEIDREKALDWLQKGAQPTDTCRAILSYTGVVYKNHLINGVKKGAFDAAEADRR
                     FDIWMNEKNAKIEAKRGNVGKAAEKALADRVAAEKKKADEIAAKLSAKLAAAAAQAEA
                     AAVPAVETPAEGEAPAADTAAE"
     gene            complement(3322..4062)
                     /locus_tag="PL106_02515"
     CDS             complement(3322..4062)
                     /locus_tag="PL106_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNA31970.1"
                     /translation="MPGRPVASAALRQSGCERSRDGVDGESREPDGDARAEPGLGEIR
                     YTTDGSEPTDTSARFFSTIEQRGSATIKAALFGGNKKLGNTTALTLTFDKATGRALQV
                     EPGPDERYDDGGIFTLVNGVSAGAKRVNHEWLGWRTSQVTVTVDLGSEEGLAHAGIGG
                     LEERSSWIHPPESVTIATSSDGQTFTDAATVRPDPKASGRMEFGTDLSGRSARYVRFT
                     VRTLPAIPDDQPGAGNLPWLFLDEVHVR"
BASE COUNT          786 a         1324 c         1378 g          795 t
ORIGIN      
        1 cacctccgcc gcgttcttgg tgtgcagctt gaacagttcc acgtgctggt cccatcggcg
       61 ttcctggtca cgtcccgcag ccttccgttg ccgaccgcct gaaggctgtc cgccacggtg
      121 atgtgcagcg aagcaccatc gtccggctca tcgctctgcg tgtccttgca cgggtaccac
      181 acgctcgcgc cgaggccctg gcaggccacg ctcatccacg ggttgccctg ggcgtcgtac
      241 gtccagatcc atccgccgtc ccaaggtggc tgtttggctt ctcgaggaac accatggtag
      301 gtgatacgga tcgtgctggc ctctcccgcc ttcaagggct ggggcaggtc cacccacagc
      361 acgttgcctt cgcgttcggt atgcgcacgc tcgtcctcaa tggtgaaact gcccctgcgg
      421 aatcggctca cgtcggccgt gacactgtcg acgacgagcg gttgttgcag atcgatctgc
      481 atgcgcaggc cgttgtcctt ggcgatgaag ccgatgatgg tggtaccgcg gatgctcttg
      541 gtggcaagat ccggctgaac gctcacgtcg tagtggagca cgtcccacca ggcacgctcg
      601 gtaccaatgg acccgcgcag ggtgtcggca ctggagaagc tcttctgacg gcggcccagt
      661 tgggcatgca cgggaaaggc cagtgcgccg atgatgatca gtagcgatac ccgcatgggt
      721 gggcaagtta gcggtgcggt cgccccggac cggacatgtt cacatatcgt tcggaatggg
      781 acgagcgtta caggccgacc ttgtccaggc ctttgatgtt cggcctgccg aggcgatagc
      841 cgatggccca gcgggctgag ccgtacatgg atccgagcat cgcaacgtga tcatcggcgt
      901 cctcgttcag gtagtaggcc agtgtgagac ctgtgtacca gcgatcgcca ttgaatccga
      961 tgccgccctt gaattccatc agggcactga cgccggtatc cgcggcgcgt tcgccgtttt
     1021 ttcccatcgt cctggtctga tcgctgtatc cggccccggc gagcaaggcg agatggatga
     1081 aacccttgtg ccagaatacg aacgtgtgcg catagccgac cgtggttccg acgatcgttc
     1141 tccgcgaggc cctgatcccg gcttcgggcg cgaaggccag tggcccggtc gttggcacga
     1201 gcgcactgtc cgcattcatg cgtgtgagcc agaaggcggc tccacacacg aacgtgcccg
     1261 cactgcgctt ttggcgctcc atctgggtga gtgccgcaac ctgcgagtag cggttcttcc
     1321 tgttgagcgc atagttcacc gaagccatcc aggtctccac accaaggtcg gggcgatgag
     1381 gccatggctg gccgtcgata tggtccggtg cgatggcgtt cggcggatcg gcatagaatc
     1441 ccgaattgta gttccagaag ccgcggaccc agatatgccg accggtgtac cccagtgcca
     1501 ctcccttgga agaggtacga ccgtagagcg gatcggcggg gtccagagcg gggatgctga
     1561 atgtggcttc caccgagagc cagcgggagt ccagggccac accatactgt gtggcgttgt
     1621 tggtggacca ggagacggca tggccgagtg tatcggagat gtccagtgtg gccaggcgat
     1681 agctgctcac caggctcaag gtgaggcgct gccggaaggt ggagatgtat gccgtatcgt
     1741 aattgggggg ggccagcgtg tcccccacca ggagcttgcg aacgaaggca ccgaattggg
     1801 cgttggaatc ggaacggatg cacaggagga gcgaaactgt gatgagcagc cttccaacgt
     1861 tgccgatcgt ggtccgacct gtgcgcatgg gtgttatact ggtagaaata tccgttccgg
     1921 tggttcaagc gcccgtacct ggtaccgcga tgttcttgcc gttcgcggtg cggcgcttcc
     1981 ggggtcgggc ttcgcgcaac cgtccatcgg atcgtgcgat atgatcggcc aggtcgcggg
     2041 cgccgggatc cgtgaggacc agtccgtagg cttgccgcag cttggcccaa gcggcccgtg
     2101 catcggcctc cttcttttcg ccgagcggcg tgatgaagat gcggatgttc ttgcgcgtgc
     2161 cttcgcgctg aacgaagcgt tcgtggacca acttgtccac ggcacgcgaa atggtggaaa
     2221 catcgagctg atgaacatgg gcaaggtcgg tggaaagaat gccgggcgcc gttcgcgtgg
     2281 tcatcaggat gaaggccatg gtgggcgtca attcaagcgg gccgaactgc tcttcggcca
     2341 gggcggtcag ggatcggccc aatgcggtgg cggcgtggaa caggcaagtg ttgtactcct
     2401 cttgaagtgt ggggaacagg gcgggtggcg gaggggggat cggggttggc atggagcgaa
     2461 agtacaccca tttgcttggc tggccaaaca atcaagatca atgccctcag tccatgaaac
     2521 atagcagcat ggaaacgctg aattcggcac cgcaatgtgg cttttgtgcg tcatagtgca
     2581 cggaaaatca tcgaatgttt gcttggccag ccacgcaaat aggtgaagca acgcaattca
     2641 ccacgatcac gcccagtcgg cggatcccca aaaatcccca tcttcgcgcc cccaattccg
     2701 ggaaccaacc aaaacagcga catgccaact cgcatccgcc ttcagagaaa aggcaaaaaa
     2761 ggccagccgt actaccacgt agtagtggct gatcaacgcg cgccccgcga cggtaaatac
     2821 atcgagagga tcggtgctta tgagcccggg cgcaaccccg cattcattga gatcgaccgt
     2881 gagaaggcgc tcgattggtt gcagaaaggc gctcagccca cggatacctg ccgtgctatc
     2941 ctgagctaca ccggggtggt gtacaagaac cacctgatca acggggtgaa gaagggggca
     3001 ttcgatgcgg ccgaggccga tcgccgcttc gacatctgga tgaacgaaaa gaacgccaag
     3061 atcgaggcca agcgtggcaa cgtgggcaag gccgccgaga aggccctggc cgaccgtgtg
     3121 gccgccgaga agaagaaggc cgatgagatc gctgcgaaac tcagcgccaa gctggcagcg
     3181 gccgctgccc aggccgaagc ggccgcggtg cccgccgtgg aaacgcccgc cgagggtgag
     3241 gcgcctgctg ccgataccgc cgctgagtga ttgttttcta tggataagga agagccccgg
     3301 tgatccgggg ctctttgctt ttcaccgtac gtgaacctcg tccaggaaca accagggcag
     3361 gtttccggca ccgggctgat catcgggaat ggccggcagc gtgcgtaccg tgaagcgcac
     3421 gtaccgggcc gatcgccccg acaggtcggt gccgaattcc atgcgtccgc tcgccttggg
     3481 gtcgggccgt acggtggcgg catccgtgaa ggtctgccca tcgctgctcg tggcgatcgt
     3541 tacgctctcg ggcggatgga tccaggaact gcgctcctcg agcccgccga tgcccgcatg
     3601 cgccagacct tcctcactgc cgaggtccac cgtcaccgtt acttgtgatg tgcgccagcc
     3661 gagccactcg tggttcacac gtttggcacc ggcactgaca ccgttgacca gggtgaaaat
     3721 acctccgtca tcgtatcgtt catccggtcc gggctccact tgcagtgccc ggccggtggc
     3781 cttgtcgaac gtcagggtga gcgcggtggt attgcccagc ttcttgttcc cgccgaagag
     3841 cgccgctttg atcgtggcgc tgccgcgctg ctcgatcgtg ctgaagaacc gtgccgaggt
     3901 gtcggtgggt tcgcttccgt ccgtggtgta tcggatctcg ccgaggccgg gttccgcgcg
     3961 tgcatcgccg tcgggttctc gcgattcgcc gtctacgccg tcgcgagagc gctcgcatcc
     4021 agactgccgc agggcggcgg acgcgacggg tcgcccgggc acaccgagcg cagatccgcc
     4081 gcatccgcgc aggcccccct gatccatgcg acgcgcgcat caccccgcac gccaccaagc
     4141 cgacccgcat ctcccgtcac ccacccgatc gcatccccga tcgcgctcga gatcgcgcaa
     4201 ccgatcgcgc tcccgcgcgc accgacaccc gagccgacgc gccgcgaccc ggccagttcg
     4261 gccaccccgg ccaccccggc cac
//
LOCUS       DAOQOC010000098         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9215_length_4268_cov_3.034892, whole genome shotgun sequence.
ACCESSION   DAOQOC010000098 DAOQOC010000000
VERSION     DAOQOC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9215_length_4268_cov_3.034892"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PL106_02520"
     CDS             <1..274
                     /locus_tag="PL106_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31971.1"
                     /translation="GWMPRDRHKPHGEKDKVLIIWRTLSADREQDNAVLDTYFTKNRL
                     EIRDKEFDVIYVNGSNNLPNLLQAGERWKVRLIEEDFHRLMWEGAE"
     gene            286..525
                     /locus_tag="PL106_02525"
     CDS             286..525
                     /locus_tag="PL106_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31972.1"
                     /translation="MDIHRDSSQQPYVEVPMGNGEFLRVTYLREGWPAEPAVRVQVRT
                     PGKPPRQGPEFPVRLVGEVVQGMLELARHEGESER"
     gene            537..1124
                     /locus_tag="PL106_02530"
     CDS             537..1124
                     /locus_tag="PL106_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31973.1"
                     /translation="MSTVIPQWNMHGVLPHIIGHGDQELRSPYRVSLPDVIHRFGNYP
                     DRTLILDGFLKFRERLHTLGLVEGYQWLDGSFVEDSELLLNRQPGDIDVVTFYKEPSG
                     LTQAALLANDADLFSFDADKVKARKDRYKVDSYMFSLTGDPKYLHAQTCLWHDTWSIR
                     RNNVRKGYVSIDLDPKDDNEATALVLDLKNKYKLP"
     gene            1121..2023
                     /locus_tag="PL106_02535"
     CDS             1121..2023
                     /locus_tag="PL106_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31974.1"
                     /translation="MNRTELENLRSERGEVLRILESIPKDRVIDRGSMEYRLRQLNEL
                     INGAGEAPIPARAIMTFRGRPVVGNNEGIYYGFATKAISTFADAVVLAERDGDDLSDL
                     GPIPGKKESGLMIKDTVIGSFGFELEEVVAKDQNPEEESRTAQALKQVGEILYNTRAS
                     DDALAQSISDVHPRVLKSLQSFLEHLASSGATASFQVNEQSFRFEQVAEVQRGAERLK
                     TENVHETTQELPGRFVGFLPNRRTFEFIKEGESDAIVGKVMKSVENASEINANLKKLI
                     TAKVKMTQVGQGRPSYVLVELPAW"
     gene            2033..>4268
                     /locus_tag="PL106_02540"
     CDS             2033..>4268
                     /locus_tag="PL106_02540"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNA31975.1"
                     /translation="MAKGRRPRATQQSHAFRNKLLLNQWLIGLFGIDPLHAHTIDDKP
                     ALPYQVLARHLKDIRTEGLGADGLHIYYHNLVSQVLALFPNAALTKEVLLRYEENIVR
                     HTEAINAQRTRPITWKYYQWLSLLFTEVYLDRYFNQPDALLEDLNAYVVRFNQHFGDH
                     APVAPYTADDLNKVCYQNATGSGKTLVMHVNLLQFRHYAHESGRADELSRVILLTPNE
                     ALSRQHIDEFADSDLAAAWFAEGRNNLFTAQTDGLRRVDVMEITKLAEKDSKQQVATR
                     SLGDQNLLLVDEGHRGLGSKTEEGAWVRNRNMLCAKGFTFEYSATFEQAVSGTPVEDD
                     YAKCVLFDYSYRWFYEDGFGKDYRIANISRAQDEELKKVYMIGALLRAFQQHLIYEEQ
                     KHTLRDFNIEKPLWVFVGHSVTGTKIKNADSDDGIMATDVARILLFLADFLHAPQAAQ
                     TRIQQLVQGDGELHGMIGANDEDLFKGAFRYLEQKMAAGMQAAQVYREILRLLFNNAD
                     GGVLRLERRKGDKGEVALHCGVSEDPFGLIYVGSAKELCDHIEQLARQNNEPIEVHAS
                     DFGEGLFEQVKESSSPVNVLIGSKKFIEGWDCWRVSSLGLMHVARSEGTQVIQLFGRG
                     VRLKGHRWSLKRSSHLPDVVRPAHIHELETLNVFGIDADFMENFRRWLVQQGLPGNER
                     VTTVRIPMNVTYDVGKRLKVLRPKRKAGEDRDVVFSKDGPVVSVGEIPDKLRTEKKFW
                     HKKQR"
BASE COUNT          990 a         1228 c         1298 g          752 t
ORIGIN      
        1 aggctggatg ccccgcgacc gccacaagcc ccatggcgag aaggacaagg tgctcatcat
       61 ctggcgcacc ctcagcgccg atcgcgagca ggacaacgcc gtgctcgata cctacttcac
      121 caagaaccgc ctggagatcc gcgacaagga gttcgacgtg atctacgtga acggcagcaa
      181 caacctgccc aacctgctgc aggccgggga gcgctggaaa gtgcgcctga tcgaggagga
      241 tttccatagg ttgatgtggg agggggcgga atgaaccagc aacacatgga catccatcgc
      301 gatagcagcc aacaacccta tgtggaagtg cccatgggca acggcgagtt cctgcgggta
      361 acctacctgc gcgaaggctg gccggccgaa cccgcggtgc gggtgcaggt ccgcacaccg
      421 ggcaagcccc cacggcaggg tccggaattc ccggtgcgcc ttgttggtga agtagttcaa
      481 ggcatgttgg agctcgcccg gcatgaaggg gagagcgagc ggtaattttg gtaccgatga
      541 gcacggtgat accacaatgg aatatgcacg gtgtgctgcc gcacatcatc ggtcacggtg
      601 accaggaatt gcgctcgcca tacagggtat cgttgcccga tgtgatacac cgcttcggca
      661 actacccgga ccggacgctc attctcgatg gatttctgaa gttcagggaa cgtcttcata
      721 ctttgggact tgtcgagggc taccaatggc ttgatgggag ctttgtggag gattcggagc
      781 tgctcttgaa ccgacagccc ggcgatatcg atgtggtgac gttctacaag gaaccatccg
      841 ggctcacgca agctgcactc ctggcgaatg atgctgatct gttcagcttc gacgcggata
      901 aagtgaaagc acgcaaggac cggtacaagg tggactcata catgttctca ctgaccggcg
      961 accccaagta cctgcatgcg caaacgtgcc tgtggcacga cacgtggtca attcgtcgga
     1021 acaacgttcg caagggatac gtgagcatag accttgatcc gaaggatgac aacgaggcga
     1081 cggctttggt gttggacctc aagaacaaat acaaactgcc atgaaccgca ctgaattgga
     1141 gaacctgagg tcagagcgcg gggaggtgtt gcgtatcctc gaaagcatcc ccaaggatcg
     1201 tgtgatcgat cgtgggagca tggagtatcg cttgcgtcaa ctcaacgagc tgatcaatgg
     1261 tgcaggcgag gcgcccattc ccgcaagagc catcatgacc ttccgcggaa ggcctgtggt
     1321 ggggaacaac gaaggtatct actacggatt tgcgaccaag gctatcagca ccttcgccga
     1381 tgctgttgta ctggctgagc gggatggtga cgatctgagc gaccttggcc ctattccagg
     1441 gaagaaagaa agcggcttga tgatcaagga tacggtcatc ggttctttcg gatttgaatt
     1501 ggaagaagtg gtcgcgaagg accagaaccc cgaagaggaa agcagaacgg ctcaggcctt
     1561 gaaacaggta ggcgagatcc tttacaacac aagagcatcg gacgacgcgc tggcacagag
     1621 catctcggat gtacatcccc gcgtgttgaa gtcgttgcaa tccttcttgg agcatttggc
     1681 ctccagtggt gcaaccgctt cctttcaagt gaacgagcaa tcgttccgct ttgagcaggt
     1741 ggctgaagtg cagcgtggtg ctgaacgtct caaaaccgag aacgtccatg agacaaccca
     1801 agaattgcct gggcgatttg ttgggttcct cccgaatcgg cgaacattcg aattcatcaa
     1861 ggagggtgag tcggatgcga tcgttggaaa agtgatgaag agcgtcgaga acgcttctga
     1921 gatcaatgcg aatctcaaga agctgatcac tgcgaaagtc aagatgaccc aagtcgggca
     1981 aggccgcccg agctatgtac tggtcgaatt gccagcctgg tagaagaagg gtatggccaa
     2041 aggacgacgc ccacgcgcga cccagcaaag tcacgccttc cgcaacaagc tcctgctgaa
     2101 ccagtggctc atcggcctgt tcggcatcga tccgctgcac gcccacacca tcgacgacaa
     2161 gcctgcgctg ccctaccagg tgctggcccg ccacctgaag gacatccgca ccgagggcct
     2221 cggcgccgat ggcctgcaca tctactacca caacctggtc tcgcaggtgt tggcgctctt
     2281 ccccaacgcg gccctcacca aggaagtgct gctgcgctac gaggagaaca tcgtgcggca
     2341 tacggaggcc atcaatgccc agcgcacccg gcccatcacc tggaaatact accagtggct
     2401 cagcctgctc ttcaccgaag tgtacctgga ccgctatttc aaccagcccg atgccctgct
     2461 ggaagacctg aacgcctacg tggtgcgctt caaccagcac ttcggcgacc atgcgcccgt
     2521 ggcgccctac accgccgacg acctgaacaa agtgtgctac cagaacgcca ccggcagcgg
     2581 caaaaccctg gtgatgcacg tgaacctcct gcagttccgc cactacgccc acgagagcgg
     2641 ccgggccgat gagctgagcc gcgtgatcct gctcaccccc aacgaggcgc tgagcaggca
     2701 gcacatcgac gagttcgcgg acagcgacct cgccgcagcc tggttcgccg aaggccgcaa
     2761 caacctcttc accgcacaga ccgatggcct gcgccgcgtg gacgtgatgg agatcaccaa
     2821 attggccgag aaggacagca agcagcaggt ggccacccgc agcctgggcg accagaacct
     2881 gttgctggtg gatgaaggac accgcggcct gggcagcaag accgaggaag gcgcctgggt
     2941 gcggaacagg aacatgctat gcgcaaaggg cttcaccttc gagtacagcg ccaccttcga
     3001 gcaggccgtg agcggcactc ccgtggaaga cgattatgcc aagtgcgtgc tcttcgacta
     3061 cagctaccgg tggttctacg aggacggctt cggcaaggac tatcgcatcg ccaacatcag
     3121 ccgcgcgcag gacgaggagc tgaagaaggt gtacatgatc ggtgcgttgc tgcgcgcctt
     3181 ccagcaacac ctgatctacg aggagcagaa acataccctc cgcgacttca acatcgagaa
     3241 gccgctgtgg gtgttcgtgg gccacagcgt caccggcacc aagatcaaga acgcggacag
     3301 cgacgatggc atcatggcca ccgacgtggc gcggatcctc ctcttcctgg cggattttct
     3361 gcatgcaccg caagccgcgc aaacgcgcat ccagcaactg gtgcaaggcg acggtgagtt
     3421 gcatggtatg atcggcgcca acgatgagga cctgttcaag ggtgccttcc gctatctgga
     3481 acagaagatg gcggcgggga tgcaagccgc gcaagtgtat cgcgagatcc tgcggctact
     3541 cttcaacaac gccgacggtg gggtgctacg cctggaacgt cgcaaaggcg acaagggcga
     3601 ggtggccctg cattgtggcg tgagcgagga tcccttcggc ctgatctacg ttggcagcgc
     3661 caaggaactc tgtgatcaca tcgaacaact cgcccggcag aacaacgaac caatcgaagt
     3721 acacgcttcg gatttcggtg aaggattgtt cgagcaggtg aaggaaagca gcagtccggt
     3781 gaatgtgctg atcggcagca agaagttcat tgaaggttgg gactgctggc gcgtgagttc
     3841 tcttggtctg atgcacgtgg cgcgcagtga aggcacgcag gtgatccaat tgttcggccg
     3901 gggcgtgcgc ctcaaaggcc accgctggag cctgaagcgc agcagccacc tgcccgacgt
     3961 ggtgcgcccc gcgcacatcc acgaactgga aacgctcaac gtgttcggca tcgatgcgga
     4021 cttcatggag aacttccgca ggtggctcgt gcaacaaggc ctacccggaa atgagcgcgt
     4081 taccacggtg cgcatcccca tgaacgtgac ctacgacgtg gggaagcgcc tgaaggtgct
     4141 gcgccccaag cgtaaggccg gggaggaccg cgacgtggtg ttctccaagg acggtcccgt
     4201 ggtgtccgtg ggcgagatac cggacaagct ccgcacggag aaaaagttct ggcacaagaa
     4261 acagcgcc
//
LOCUS       DAOQOC010000099         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9234_length_4264_cov_3.217629, whole genome shotgun sequence.
ACCESSION   DAOQOC010000099 DAOQOC010000000
VERSION     DAOQOC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9234_length_4264_cov_3.217629"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PL106_02545"
     CDS             complement(<1..1122)
                     /locus_tag="PL106_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31976.1"
                     /translation="MRIAMIILASVVASAAQALSVQCWTYPDVCNYGNGQAGASATGG
                     APPYTYSWDNGGTGANLTGLVPGTYTVTVTDNLGAQASCAATVTASAELTVYGGPGTP
                     TCPGTCTGSASFDKTLFGGVAPYTYSVPPANEDANLVYYGSVCWYAEGNMLTVTDANG
                     CNSTVQLGVPNSYYSQVELLQIMPSCQGAAGQITFQLGFTMLTTLRVLDDQLNIVYQD
                     PAPPESGIVVVGDLAPGDYFIRCYSNEIPGEWCYGETPFTIPDLDTDCGRIMGRLFID
                     ADDDCQFDQPDEETAMPYRVLVVEPGPLYGITDGLGEYVINLEEGSYTIENQDPDLFP
                     LCPATEPVPFDITAFAPVVMQDLADSSSVAPDLELTCTHS"
     gene            complement(1125..3356)
                     /locus_tag="PL106_02550"
     CDS             complement(1125..3356)
                     /locus_tag="PL106_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31977.1"
                     /translation="MNRPLRTLLLLAICHTTPLFALTVNISVNVFPTCNYPSGQLHAN
                     ASGGVGPYSYLWSNGATTEYLFDVAPGFYSVTVTDANSDQATDDHTLVGQPYSLSDLD
                     SNPVLCSNGLTGVVLPGPGYDLASGLYMGPLPYYVEGQLMQEHVIEEFMTPVDTVYTW
                     VEQATLFDTFVTYTFEDGAGCTGTLMESIGYMVEWPQLDILDIQGACAGGSNGSITFQ
                     TGLEGHGQWTQVKIETIGGQYLMSFGAGAEVETNTWNLPAGDYQLTQFNSESGFLVGS
                     GCHLSTPFTIPDLGNGCGNISGTVYMDNNEDCARQGGEPGAAGVVLEILPGPVYTITS
                     NDLYGNAGHYSVNLPLGAYTVQQQSAVLEDHCLVAPAPFTLNVGNALQTLSVADTALV
                     PLDASVSLASGAARPGFQLALAARIANNTLASTGASTLTVTFDPVLSFLSANPAPSNI
                     VGNTLTWNVAAFSSFQERTVHVQLQIPPDILLLGTDLLFNAAFATGNADAVLANNSTS
                     LATTVTGSYDPNDKTAHTSSGASDELYYIDQDEWIDYTIRFQNTGTDTAFNIVITDTI
                     PTELDLGTLEVGASSHPNSLSIRDGNVLRWAFYNIQLPDSNVNEPASHGFVSFRIKPR
                     LPLAPTTPIENIANIFFDLNPPVITEPSVLTAEFSTSITHADASLLFISPNPTSQQMM
                     VRAGSESGRLSIHVPDGRLVLSEWISGGQGIIDVSGLPAGPYVLVWEGGTVRSQTKLI
                     KAD"
     gene            complement(3622..>4264)
                     /locus_tag="PL106_02555"
     CDS             complement(3622..>4264)
                     /locus_tag="PL106_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA31978.1"
                     /translation="LICPGDSSEAVGPAGFEDYLWSNGSTDQVAWSHGEPMVLTVLND
                     DGCISNSDTLFYTLLPEPGTPVITDAGGVLSSTPADGYQWYLNGTEISGATGQEHAAT
                     ENGDYTVVVTAPSGCTAESDPYTLLNVGVPGVASTGFRVWPIPAEGTLFVTVDATTPL
                     AYAIIDASGKEVKRGTLRPGGTSLIDLNGLVPGSYTLVVRSGERSNQRVFVVR"
BASE COUNT          821 a         1292 c         1326 g          825 t
ORIGIN      
        1 cgagtgcgtg caggtgagtt ccaggtcggg tgcaacggag ctgctgtccg ccaggtcctg
       61 catcaccacc ggcgcgaacg ccgtgatgtc gaagggcaca ggctcagtgg ccgggcacag
      121 cgggaagagg tcgggatcct ggttctcgat cgtgtaggat ccttcttcga ggttgatgac
      181 atactcccct agcccatcag taatgccata caggggaccc ggctccacca ccagtacgcg
      241 gtagggcatg gccgtctcct catccggctg atcgaattgg caatcgtcgt cggcatcaat
      301 gaacaagcgt cccatgatgc ggccgcaatc ggtgtcgagg tcgggtatcg tgaaaggcgt
      361 ctcgccgtag caccactcgc cggggatctc gttgctgtag cagcggatga agtagtcgcc
      421 gggcgccaga tcgcccacga caacgatgcc gctctccgga ggcgccggat cctggtacac
      481 gatgttcagt tgatcatcca gcacacgcag ggtggtgagc atggtgaagc cgagttggaa
      541 cgtgatctgc ccggccgccc cttggcaact gggcatgatc tgcagcagct ccacctgtga
      601 gtaataggaa ttgggcacgc ccagctgcac ggtgctgttg cagccattcg catcggtcac
      661 cgtcagcatg ttcccttcgg cataccagca cacgctgccg tagtacacca agttcgcgtc
      721 ctcattcgcg ggcggcacgc tgtacgtgta gggcgccact ccgccgaaga gcgtcttgtc
      781 gaacgacgct gagcccgtgc aggtgccggg gcaggtcggc gtgcctgggc caccatagac
      841 cgttagctcc gcgctcgccg tcactgtggc cgcgcagctc gcttgcgccc ccaagttgtc
      901 cgtcaccgtc accgtgtagg tgccaggaac aaggccagtg agattcgcac ccgttccgcc
      961 attgtcccag gaataggtgt acggcggcgc tccaccggtc gcgctcgccc cggcttgtcc
     1021 gttgccgtag ttgcagacat ccgggtatgt ccagcactgc acgctgagcg cctgcgccgc
     1081 tgatgcgacg acactggcaa ggatgatcat cgcgattctc atgttcaatc cgctttgatg
     1141 agtttggtct gcgaacgcac tgtacctcct tcccacacga gcacgtaagg cccagcgggc
     1201 aggccgctga catcgatgat gccttgcccg ccgctgatcc attcgctcag gaccaagcgg
     1261 ccatcgggca cgtgaatgct caagcgtccg ctctccgatc cagcgcgcac catcatctgt
     1321 tggctcgtcg ggttcgggct gatgaatagc aaggaggcat ctgcgtgtgt tatggaggtg
     1381 ctgaactctg cggtgagcac gctcggctcg gtgatcaccg gcgggttgag atcgaagaaa
     1441 atgttggcga tgttctcgat cggcgtggtg ggtgccaacg gaagtcgtgg cttgatccgg
     1501 aagctcacga agccgtggct cgcaggctcg ttcacgttgc tgtccggcaa ctgaatgttg
     1561 taaaaggccc agcgcagcac gttgccatcg cggatggaga gcgagttggg atgcgaggat
     1621 gcaccaactt cgagggttcc caggtcgagt tccgtaggta tcgtatctgt gatcacgatg
     1681 ttgaaggcag tgtcggtacc ggtgttctgg aagcggatgg tgtagtcgat ccactcgtcc
     1741 tgatcgatgt agtacagctc gtcgctcgcg ccgctgctgg tgtgcgccgt cttgtcgttg
     1801 gggtcgtagc tgcccgtaac ggtggtggcg agggaagtgc tgttgttcgc cagcaccgca
     1861 tcggcattgc ccgtggcgaa ggctgcgttg aaaagaagat ccgtgccgag caatagaata
     1921 tcgggtggta tctgcagttg cacatgcacg gtgcgctctt ggaaggaact gaacgcggcg
     1981 acgttccagg tgagggtgtt gccgacgatg ttcgaaggcg ccgggttcgc agagaggaac
     2041 gacagcaccg ggtcgaaggt cacggtcaag gtactcgcac ccgtgctggc caacgtgttg
     2101 ttcgctatcc gtgcggcaag cgcaagttgg aagcccggac gcgccgcacc gctcgccaac
     2161 gaaacgcttg cgtccagcgg cacaagagcg gtatcggcca cgctgagcgt ttgcaacgca
     2221 ttacccacgt tcagggtgaa tggggccggt gcaacaaggc aatgatcctc cagcaccgcg
     2281 ctctgctgct gcaccgtgta ggcccccaag ggcaagttca cgctgtagtg gccggcattc
     2341 ccgtacaagt cgttgctggt gatggtgtac accggaccgg gcaggatctc gagaaccacg
     2401 cccgctgcgc ccggctcacc accctgccgc gcacaatcct cgttgttgtc catgtacacc
     2461 gtgccgctta tgttgccgca gccattgccc agatcgggta tggtgaaggg tgtgctcaag
     2521 tggcagccgc tgcccaccag gaagccggac tcactgttga actgggtcag ttggtagtcg
     2581 cccgcaggga ggttccacgt attcgtttcc acttcagcac ctgcaccgaa ggacatgagg
     2641 tactggccac cgatcgtttc gatcttcacc tgggtccact gcccatgacc ttcgagaccg
     2701 gtttggaagg tgatggaacc attgctccca ccggcacacg ctccttggat atccaggatg
     2761 tccaactggg gccattccac catgtagccg atggactcca tgagcgtacc cgtgcaaccc
     2821 gccccatcct cgaacgtgta agtgacgaac gtgtcgaaca gtgtggcctg ctccacccat
     2881 gtgtatactg tatccaccgg ggtcatgaac tcctcgatca catgctcctg catcagctgg
     2941 ccttcgacgt agtaaggtag cggccccatg tatagcccgc tggccagatc gtatccggga
     3001 cctgggagca ccacgccggt cagcccgttg ctacagagca ccgggttgct atccagatca
     3061 ctcagcgaat agggttgccc aaccaacgtg tgatcgtcgg tcgcctggtc gctgttggca
     3121 tcggtcaccg ttaccgagta gaagcccgga gccacatcga agaggtattc cgtggtagcg
     3181 ccattgctcc agaggtagct gtagggccca acaccgccgc tggcattagc gtggagctgg
     3241 ccgctggggt agttgcaggt gggaaacaca ttcaccgaga tattcaccgt aagggcgaac
     3301 agcggcgtgg tgtggcagat agcgaggaga aggagagtgc gtaggggtcg gttcatagcc
     3361 agatggtgca acacttcacc gaagaagacg cagagaaggt ggttggatgc ctgaaggtag
     3421 gaacattttg acgacagaag cccagagaac ggatgctccc acaggaccac cacaggcacg
     3481 gaccactgcg gcatccgtac cggaacatgg gcacacgcag cagctaccag ccgggcttgg
     3541 tgaaccggtc tgaacggatc cacgccgata cgggcggatc ccttcgccca gtacgccccg
     3601 agcggggatc actcggatcc atcagcggac gacgaacacg cgctggttgg agcgctcgcc
     3661 cgaacgcaca accaaggtgt acgagccagg gaccaggccg ttcaggtcga tgaggctggt
     3721 accacccggc cgcagggtgc cacgcttcac ctccttgccc gacgcatcga tgatggcgta
     3781 tgccagcggc gttgtggcat ccactgtcac gaacaaggtg ccttccgcag ggatcggcca
     3841 cacacggaaa cccgtgctcg ccacacccgg cacccccaca ttcagcaggg tatacggatc
     3901 cgactccgcg gtgcaaccgc tcggcgccgt gaccactacc gtatagtccc cgttctccgt
     3961 ggccgcgtgc tcctgaccgg tcgcacccga tatctcggta ccgttcaggt accattggta
     4021 gccatccgcc ggggtgctgc tcaagacacc tcccgcatcg gtgatcaccg gcgtgcccgg
     4081 ctccggcaac agcgtataga acagtgtgtc actgttgctg atgcatccgt catcattgag
     4141 caccgtgagg accatgggct cgccatggga ccatgcgacc tggtcggttg aaccgttgct
     4201 ccagagatag tcctcgaagc cggccgggcc gaccgcctcg gagctgtccc cggggcagat
     4261 gagc
//
LOCUS       DAOQOC010000100         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9245_length_4260_cov_3.455648, whole genome shotgun sequence.
ACCESSION   DAOQOC010000100 DAOQOC010000000
VERSION     DAOQOC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9245_length_4260_cov_3.455648"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PL106_02560"
     CDS             <1..786
                     /locus_tag="PL106_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31979.1"
                     /translation="GGWVTPGFQQAPPRTTGVLPKVFLEGPFVSGSGTMTDGLRSGGL
                     IPLTSPYVNSAQFPQINGGGGETIAPSVLTATGNNAIVDWVYVQLRNGANSSQVLRTR
                     SALVQRDGDVVDMDGVSQLTFVAPAASYHVAVFHRNHLPVMSANPISLSSVGVPVNFT
                     SPSLLTYGTNAQKTIGTVRALWAGDVNTDGIIKYTGGSNDRDPILVTVGSTAPNNTVT
                     AYSTRDVNLNGQVKYTGGSNDRDPVLVNVGSTTPNNTRVRQLP"
     gene            819..2132
                     /locus_tag="PL106_02565"
     CDS             819..2132
                     /locus_tag="PL106_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31980.1"
                     /translation="MKHLHLLLVVVGLSIAPALQAQYQQFHSMPYQGVARNASGQPVA
                     NQNIRVRFILQYGLSNAYIESQLLTTNAFGVFNAQIGDGTPEPATFPSFNAIDWWGGS
                     SAGLQVWIDITGGTNYVLAGSTPLRSVPFATLSEQAFALLDSSLYSATNRVFMKPTAR
                     MGLGTETPQNLLDVEGSMVIGSSYSGTNTAPVNGMLVQGNVGIGTTVPQQRLDVQGNA
                     LVRGTEFDSIGDSAVLYVGDTDSRVQNTYGTGLQLYTYPGSSPAITIETGTNNVGIGT
                     ELPVAKLDVAGNVRIADGTQGAGKVLTSDASGNATWSAVVSSGSYTPTLSGATGFVSP
                     GTMECTYSRVGPLVTVACSRAANVQEFGAGTNTVNLSLPPGLPVVFTGRPTGTFASDH
                     YRNGTDTSIGLVTYNNSTSVKLMIKNSSPGLASAYCSFTYNTDAP"
     gene            complement(2152..3648)
                     /locus_tag="PL106_02570"
     CDS             complement(2152..3648)
                     /locus_tag="PL106_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA31981.1"
                     /translation="MRNRTTLLACLLTALLHAQPQLDSLDAYIARSVRSFDQPGLAVG
                     IVKDGALIWSKGYGKLDLAKPDPVTPNSVFYCASLSKAFTACAIGLLVDEGKLAFDDP
                     VRKHLPWFKTPDPYVSENFLVRDLLCHRSGWITFDGDLLWYGTDLSQREILERHSKEP
                     FTYSFRANYGYSNLMLIAAAQVIEAVSGMTWDRFITTRIFQPLGMDRSRVETSDLASM
                     TDVAMPHVRKGQDPDAPQKSMPYQPLQGADGACGVLSTVSDLAKWDAMWMNEGRAGNK
                     EFISERTFNTLTSVQLPTRGQGAGVALGWFVEENHGAKTITHSGGMPGFILNHAVVPD
                     KDLAVIALGNGESYSVFAITEKILDLYLGDGKADPTTDLLRYVQADRTEQAKRRQDRI
                     EARIKGTKPTVDVIALNGTWTDKVYGDAVISSMNGSPVLTFSHAKQLFTGALEHWHYD
                     TWKWNHADPFLEPGYVTFEFDTDHKVTGFKVDLHSPDFHFWKLDFRRQ"
     gene            3733..4128
                     /locus_tag="PL106_02575"
     CDS             3733..4128
                     /locus_tag="PL106_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31982.1"
                     /translation="MRRVLIIVSLLILVVAVLFFYFRYAWVFGEGAKSGELNFVVHKG
                     WLWKTYEGRLIQTGIKGQAGSMQSNEFNFSVVDPKVAEQLMVNSGKVFNLHYLEYQGA
                     LPWRGQSVYVVDSIISMTGTRDPAGLVVP"
BASE COUNT          816 a         1270 c         1333 g          841 t
ORIGIN      
        1 ggtggctggg taacgcccgg attccagcag gcaccaccgc gaacgaccgg ggtcctcccc
       61 aaggttttcc tggaagggcc ctttgtgagt ggcagtggga ccatgaccga tggcttgcgc
      121 agcggaggac tcattccgtt gacatcgccg tacgtcaaca gtgcgcagtt cccgcagatc
      181 aatggcggag gtggggaaac catcgcccct tccgtgctca cggccacagg gaacaacgcc
      241 attgtggatt gggtgtatgt gcagttgcgc aatggcgcca acagttcgca ggtgctccgc
      301 acgcgaagcg ccctcgttca gcgcgatggc gatgtggtgg atatggatgg cgtgagccag
      361 ctgacgttcg tggcgccagc ggcgtcctac catgtcgcgg tattccaccg taaccacctg
      421 ccggtgatgt cggcgaaccc catcagtctg tcgtccgtgg gcgtgccggt gaatttcacc
      481 agcccatcgc tgctcacgta cggcaccaac gcgcaaaaga cgatcggcac ggtgcgggcc
      541 ttgtgggcgg gggatgtgaa cacggatggg atcatcaagt acaccggtgg ttccaatgat
      601 cgcgatccga tcctggtgac cgtgggcagc accgcgccga acaacaccgt tacggcgtac
      661 agcacgcggg atgtgaacct gaacggccag gtgaaatata ccggaggcag caatgatcgc
      721 gatcccgtcc tggtgaacgt gggcagcacc acgcccaaca acacccgcgt acgccaactt
      781 ccatgatccc ttcgaagaag cactcgaaaa aaatccccat gaagcacctt cacctcctgc
      841 tggtcgtagt cggcctgtcg atagcccccg cgctccaggc ccagtaccaa cagttccact
      901 ccatgcccta ccaaggtgtg gcgcgcaatg ccagcggtca gccggtggcc aaccagaaca
      961 tccgggtgcg gttcatattg cagtacggac tttccaacgc gtacatcgaa tcgcaactgc
     1021 tcaccacgaa cgccttcggg gtgttcaacg cgcagatcgg ggacggcacc ccggaaccgg
     1081 ccaccttccc gtccttcaac gcgatcgact ggtggggcgg aagttccgcc gggctccagg
     1141 tctggatcga catcacgggc ggtacgaact acgtgcttgc gggcagcaca ccgttgcgga
     1201 gcgtgccttt cgccacactc tccgagcagg cgttcgcctt gctggacagt tcgctctact
     1261 ccgcaacgaa ccgggtattc atgaaaccca ccgcccggat gggactgggc accgagaccc
     1321 cgcagaacct gctggacgtg gaaggaagca tggtgatcgg cagcagctat tcaggaacga
     1381 ataccgcacc ggtgaacggg atgctggtgc aaggcaacgt gggcatcggt accactgttc
     1441 cccagcaaag gctggacgtg cagggcaacg cgctcgtgcg cggcaccgaa ttcgattcga
     1501 tcggcgattc cgctgtgctc tacgtgggcg acacggactc ccgcgtgcag aatacgtacg
     1561 gtacggggct gcagttgtac acctaccctg gttccagccc tgctatcacc atcgaaacgg
     1621 gcacgaacaa tgtgggcatc ggcaccgagt tgcccgttgc caaattggat gtagcgggca
     1681 acgtgcgcat tgccgacggc acccaaggcg caggcaaagt gctcacttcc gatgcgagcg
     1741 ggaatgccac ctggtccgcc gtggtcagct ccggtagtta tacgccgacc ttgtccggtg
     1801 caacgggttt cgtgagtcct gggaccatgg aatgcacgta cagccgggtt ggcccactgg
     1861 tgaccgttgc ctgtagtcgc gccgccaatg tgcaagaatt cggggcaggc acgaacaccg
     1921 tgaacctctc gcttcctcct ggcctgccgg tggtattcac tgggcgcccc actgggactt
     1981 tcgcgagcga ccactaccgg aacggcacag acaccagcat tggcctggtg acctacaata
     2041 acagtacgag cgtcaagttg atgatcaaaa actcatcgcc agggctggcc agcgcctact
     2101 gttcattcac ctacaatacc gacgcgccct gacgaaggag gccggccgct ttcactgccg
     2161 ccggaagtcc agcttccaga aatggaagtc cgggctgtgc aggtccacct tgaaaccggt
     2221 gaccttgtgg tcggtatcga actcgaaggt gacgtacccg ggttccagga aggggtcggc
     2281 gtggttccac ttccaggtat catagtgcca gtgttcgagg gcaccggtga acagttgctt
     2341 cgcgtgggag aaagtcagaa cgggtgagcc gttcatcgaa ctgatcaccg catcgccgta
     2401 cactttgtcc gtccatgtgc cgttcaatgc gatcacatca acggtaggtt tcgtgccttt
     2461 gatgcgggct tcgatgcgat cctgcctccg cttggcctgt tcagtgcggt cggcttgcac
     2521 atagcggagc aggtcagtcg tgggatcggc cttcccatcg ccaagataga gatcgaggat
     2581 cttttcggtg atggcgaaga cgctgtagct ctcgccgttg cccaaggcga tcacggcgag
     2641 gtccttgtcg ggcaccacgg catggttgag gatgaagccg ggcatgcccc cgctgtgggt
     2701 gatggtcttt gcgccgtgat tctcctccac gaaccatccg agtgccacgc ctgcgccctg
     2761 gccacgcgtg gggagttgca cactggtgag cgtattgaat gttcgttcgc ttatgaactc
     2821 cttgttgccc gcccgccctt cgttcatcca catggcatcc cacttggcca gatcgctaac
     2881 ggtgctcagc acaccgcacg ccccgtcggc tccttgcaag ggctggtagg gcatgctctt
     2941 ttgcggcgca tccggatcct gccctttgcg cacgtgcggc atggccacgt cggtcatgga
     3001 tgcgaggtcg gatgtttcca cgcggctgcg atccatgccc agtggttgga agatgcgcgt
     3061 ggtgatgaag cggtcccaag tcatcccact tacggcctcg atcacctgag cggctgcgat
     3121 gagcatgagg ttgctgtatc cgtagttggc ccggaagctg taggtgaacg gttcttttga
     3181 atgccgttcg aggatctcgc gctgcgacag gtcggtgccg taccacagga ggtccccgtc
     3241 gaaggtgatc catccgctgc ggtggcagag caggtcgcgc acgaggaagt tctccgatac
     3301 atacggatcg ggagttttga accagggcag gtgcttgcgc acgggatcat cgaaggccaa
     3361 cttgccctca tcaaccagca ggccgatggc gcacgcggtg aacgccttgc tcaggctggc
     3421 gcaatagaac acggagttgg gcgtcaccgg atcgggcttg gccaggtcga gtttgccgta
     3481 gcccttgctc cagatgagcg cgccatcctt cacgatgccc acggcgagcc cgggctggtc
     3541 gaatgaccgg acggagcggg cgatgtacgc atcgagggag tcgagttgtg gttgcgcatg
     3601 cagaagggcc gtgagcagac aggcgaggag cgtggtgcgg ttgcgcatga tggttgccgg
     3661 ttgatcggcg aaggtggtga tcagcggggg tattcctgcg gtagcttcgc cgcgcaacct
     3721 accgctcccc ccatgcgccg tgtcctgatc atcgtttccc tgctcatcct cgtcgtcgcc
     3781 gtgctctttt tctatttccg gtatgcgtgg gtgttcggcg aaggggccaa gagcggcgag
     3841 ctgaatttcg tggtacacaa aggctggttg tggaagacct acgagggccg gttgatccag
     3901 accgggatca aagggcaggc gggaagcatg cagagcaacg aattcaactt ctcggtggtg
     3961 gacccgaagg tggccgagca attgatggtg aacagcggca aggtgttcaa cctgcactac
     4021 ctggaatacc aaggggcgct gccctggcgg ggccagagcg tgtacgtggt ggactcgatc
     4081 atcagcatga ccgggacgag ggatcctgcc gggctggtgg tgccttgaaa tgcaccccgg
     4141 tcgatgaatc cgcgcgttcg ggcaagtcgc cggatctact cggatcccac actcatttca
     4201 gcgcccggac cgatagcctc ctttttcccg cctttcaatt cggtggtggc atcgatgcct
//
LOCUS       DAOQOC010000101         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9294_length_4243_cov_3.271490, whole genome shotgun sequence.
ACCESSION   DAOQOC010000101 DAOQOC010000000
VERSION     DAOQOC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9294_length_4243_cov_3.271490"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1602)
                     /locus_tag="PL106_02580"
     CDS             complement(190..1602)
                     /locus_tag="PL106_02580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA31983.1"
                     /translation="MTIKQIYTGCLAHAAYYLESNGEAAIFDPLREVRPYIERATADK
                     ARITYVFETHFHADFVSGHLDLAANTGAIIVYGPTARPAFAAHVATDSEVFRIGKAKV
                     KAIHTPGHTMESTTYLLIDEEGREHGIITGDTLFIGDVGRPDLAQHVIADLTEEKLAG
                     HLYDSLRNKIMPLDDQLIVYPNHGAGSACGKMMSKETTDTLGNQKRTNYALDPKLSKE
                     DFIKAVLSGLTPPPGYFPMNVLMNIRGCESLDSVMARGKRPLSVAEFEVVANEERPLV
                     LDTRSGAEFAQGFIPNSINIGLDSNFAMWVGEMIPDIKQAILLVAAPGKEEESIIRLS
                     RVGYDNPIGYLDGGFEAWKRAGKGIDSVTRITADEFAVRYADKPPVFDVRKKSEFDSE
                     HVVGAINTPLNRINDHLAEFPKDKPFILHCAGGYRSMIAASILKARGWDNLVDVQGGF
                     TAIKNTPVERTTFSFPTTLL"
     gene            complement(1904..2551)
                     /locus_tag="PL106_02585"
     CDS             complement(1904..2551)
                     /locus_tag="PL106_02585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNA31984.1"
                     /translation="MEITNHMPRIGDQAPDFEALTTHGPLKFSEYIKGGWAILFSHPA
                     DFTPVCTTELSGFAQHREWFAERNTKLIGESIDSIHSHVAWVHAVREKTGVSMDFPII
                     ADIDMKVARLYGMLHDNASSTASVRAVFFIDPRGIIRLIMYYPLNVGRNMDEIMRVLE
                     AMQTADANGCAMPLNWKKGDKVIVPPPRTLKELDARLGEKNIELIDFYLAKKELA"
     gene            2678..2884
                     /locus_tag="PL106_02590"
     CDS             2678..2884
                     /locus_tag="PL106_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNA31985.1"
                     /translation="MKPNMGSTDRVIRICAAIAIALLYFTGVLTGTLGIGLLVLAIVF
                     VFTSFINFCPLYTFFGINTRKDRS"
     gene            2881..3081
                     /locus_tag="PL106_02595"
     CDS             2881..3081
                     /locus_tag="PL106_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31986.1"
                     /translation="MRRPWVITTVGAALGAAAGWFYWYQWGCTNGCAITGNPMNSTLY
                     GALLGGLLFNSFKKPAGQGARG"
     gene            3161..3463
                     /locus_tag="PL106_02600"
     CDS             3161..3463
                     /locus_tag="PL106_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA31987.1"
                     /translation="MLRSLFGSGPKVDLKAVLEQGATILDVRTPTEFAQGHVKGAMNV
                     PVDQLQSHLARVPKNKPVITCCKSGMRSGMAEGILRKQGYEAYNGGPWTHVQKLKG"
     gene            3699..4004
                     /gene="trxA"
                     /locus_tag="PL106_02605"
     CDS             3699..4004
                     /gene="trxA"
                     /locus_tag="PL106_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNA31988.1"
                     /translation="MGKPTTFQELIASDKPVLVDFFAEWCGPCQMMPPILNEFKGMMG
                     EQATVIKVDVDKNPGVAQAYQVRGVPTLIIFKKGEPVWRRSGVMNAAQLKEAMMPHL"
BASE COUNT          772 a         1286 c         1303 g          882 t
ORIGIN      
        1 accttcgcgg cggagcgtgc gcaggccgtg ctggtgaacg cggccgcgga tcgcgactgg
       61 ggggatcgcg tggtgtacta tgccgatccc gatgggcatg tgatcgccct ggcccaacgg
      121 atgtgagcac cacctcggtc cgatcgcacc ggtccgttcg tgccgatcgt tccgaacctc
      181 aggtacggct cagagcaacg ttgtggggaa gctgaacgtg gtgcgttcca caggcgtgtt
      241 cttgatggcc gtgaaaccgc cctgcacgtc cacaaggttg tcccagcccc gggctttgag
      301 gatgcttgcc gcgatcatgc tccggtaacc acctgcacag tgcaggatga acggcttgtc
      361 cttggggaac tcggcgaggt gatcgttgat gcggttcagt ggcgtgttga tggcgcccac
      421 cacatgctcg ctgtcgaact cactcttctt gcgcacatcg aatacaggcg gtttgtcagc
      481 ataccgcacc gcgaattcat ccgctgtgat ccgcgtcacc gaatcgatcc ccttgccggc
      541 cctcttccac gcctcgaagc cgccatcgag atagccgatg ggattgtcgt agcccacacg
      601 gctcaaccgt atgatgctct cctcctcctt gccaggtgcc gcgaccagca ggatcgcctg
      661 cttgatatct gggatcattt cgcccaccca catggcgaag ttgctgtcca gcccgatgtt
      721 gatgctgttg gggatgaagc cctgtgcgaa ttccgcaccg gagcgtgtat caagcacgag
      781 cgggcgctcc tcattggcca ccacctcgaa ttcggccacg ctcagcgggc gtttgccacg
      841 tgccatcaca ctgtcgaggc tctcgcaccc ccgaatgttc atcagcacgt tcatggggaa
      901 gtagcccggc ggaggtgtga ggccgctgag cacggccttg atgaagtcct ccttcgacaa
      961 cttcggatca agggcatagt tggtgcgttt ctggttgccg agggtgtcgg tggtctcctt
     1021 gctcatcatc ttgccgcagg cgctgcccgc cccgtggttg gggtacacga tcaactgatc
     1081 gtccagcggc atgatcttgt tgcgcaggct gtcgtacaga tgaccggcga gcttttcctc
     1141 ggtaaggtcg gcgatgacat gctgcgccag gtcgggccgt cccacatcac cgatgaagag
     1201 cgtatccccg gtgatgatac cgtgctcccg accttcctca tcgatcaaga gataggtcgt
     1261 gctctccatg gtatggcccg gggtgtggat ggccttcacc ttggccttgc cgatccggaa
     1321 cacctcgcta tccgtggcca cgtgcgcggc gaaggccggc ctggcggtgg ggccgtacac
     1381 gatgatggca cctgtgttcg cggccagatc caagtggccg ctcacgaagt cggcgtggaa
     1441 gtgcgtctcg aaaacatacg tgatcctggc cttgtcggcg gtggcacgct cgatgtaggg
     1501 tcgcacttct cgcagcgggt cgaaaatggc cgcctctccg ttactctcca gatagtaggc
     1561 cgcatgggcg aggcatccgg tgtagatctg cttgatggtc atggcagcgg ggtgttcggg
     1621 gtcgggtacg ggatccaaca ggctgaaggt acgatgatcc cgggatccac ggtgccgatc
     1681 ccggatggtc atcatggcag gggatcttcc aaggggcagg gtacggtatc ggtctcaggc
     1741 gtccgggatc gagcgtctcc cggtggaccg ccacatcaca gggcgtccca tacccgatca
     1801 tacgcatcat ttccatctgc atccgtgcac gtacaaggga acggtgtact cgccataccg
     1861 aaaggccccg gaaggagccc ttcgttccgc ggccggatcg ggctcaggcc agttccttct
     1921 tcgcgaggta gaaatcgatg agttcgatgt tcttctcccc caggcgggca tccagttcct
     1981 tgagcgttct gggcggcggc acgatcacct tgtcgccctt cttccagttc aacggcatgg
     2041 cacagccatt ggcatcggcg gtctgcatgg cttcgagcac gcgcatgatc tcgtccatgt
     2101 tgcggcccac attgagcggg tagtacatga tcaggcggat gatgcccctc ggatcgatga
     2161 agaacacggc acgtacggag gcggtgctgc tggcgttgtc gtgcaacatg ccgtagaggc
     2221 gggccacttt catgtcgata tccgcgatga tcgggaagtc catggacacg ccggtctttt
     2281 cgcgtacggc gtgtacccag gccacgtggc tatggatgct gtcgatgctc tcgccgatga
     2341 gcttggtgtt gcgctcggcg aaccactccc tgtgctgcgc gaatccgctg agttcggtgg
     2401 tgcatacggg agtgaagtcc gcagggtggc tgaacaggat ggcccaaccg ccctttatgt
     2461 actcgctgaa tttgagcggg ccgtgcgttg tgagcgcttc gaaatcgggg gcctgatcgc
     2521 cgatacgggg catgtggttc gtgatctcca tggcgcaaag gtcgccggtg cgtccgcgct
     2581 tccagtgacg atcgtcacgg gaccggcact tcgtgatctt cctcaccgat cacggatgat
     2641 ccccacccga cctttgcgcc atctgaacgc tgtccggatg aaaccgaaca tgggctccac
     2701 cgaccgggtg atccgcatct gtgccgcgat cgcgatcgcc ttgctgtact tcaccggcgt
     2761 gctgacgggc accttgggta tcggactgct ggtactggcc atcgtgttcg tgttcacgag
     2821 cttcatcaac ttctgcccgc tgtacacgtt tttcggcatt aacacccgca aggaccggtc
     2881 atgaggcgac cctgggtcat caccacggtc ggtgcggcgc tgggcgccgc cgccggatgg
     2941 ttctattggt accagtgggg ctgtaccaac gggtgtgcca tcaccggcaa cccgatgaac
     3001 agcacattgt acggtgcatt gctgggcggg ctgttgttca actcattcaa gaaaccggca
     3061 ggacaaggag cccgtggatg agctcatggg caggccaccc acgcacgcac accaccgacc
     3121 ctcactcccg ctcaccacca catcattcac cacattgaac atgctccgtt cccttttcgg
     3181 ttccggtccc aaggtggacc tcaaggcggt ccttgaacaa ggcgccacca ttctcgacgt
     3241 gcgtaccccg accgagttcg cccaaggcca cgtgaagggc gccatgaacg taccggtgga
     3301 ccagttgcaa agccaccttg cacgggtgcc caagaacaaa ccggtgatca cctgctgcaa
     3361 gagcggcatg cggagcggca tggccgaggg catcctgcgc aagcaagggt atgaggcgta
     3421 caacggcggt ccgtggaccc atgtgcaaaa gctgaagggg tgatgagtgc gttgctcgcc
     3481 agttggggtt ggatgcgttg gatccggctt gggcttgccc tggccttctt gatggcaggc
     3541 gtccagaacg gggatcccgt ggcttacatg gcagcggctt tcttcggtgt tcaggcgatc
     3601 ttcaacctcg gatgctgcgg tgcgacctgc agaactgaca gccgaacggc caaacccgga
     3661 actggtacga acgttgtgtg tgaggaagcg gaatgaccat gggcaagcca acgaccttcc
     3721 aggaactgat cgccagcgat aagcccgttc tggtggattt cttcgccgaa tggtgtggtc
     3781 cctgccagat gatgcccccg atcctgaatg agttcaaggg catgatgggc gagcaggcca
     3841 cggtgatcaa ggtggatgtg gacaagaacc cgggtgtggc ccaggcctac caggtgcgcg
     3901 gcgtacccac gctgatcatc ttcaagaaag gcgaacccgt gtggcgccgt tccggtgtga
     3961 tgaacgctgc gcaattgaaa gaggccatga tgccgcatct ttgagaacca taagacaccc
     4021 gttcgcggat ccggggccgc gagttgaacc ccgctcgaac acccgtccgg atccgatccc
     4081 tctccccgac accccgaccc atgcgcccta ccctcctcct cgcgaccctg gcttccgctc
     4141 tttcactcac atgctgttcg gcacaatcgg accgcatggt gaacgtggcc gcgttgcaag
     4201 ccgagctgaa acctggaaat acggtacaat tggttgacgt acg
//
LOCUS       DAOQOC010000102         4221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9380_length_4221_cov_3.315891, whole genome shotgun sequence.
ACCESSION   DAOQOC010000102 DAOQOC010000000
VERSION     DAOQOC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9380_length_4221_cov_3.315891"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PL106_02610"
     CDS             <1..1262
                     /locus_tag="PL106_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA31989.1"
                     /translation="MRQAQGDMRQAQGDMRQARGDIQVEFVGARKESYSRNSRKPEVE
                     PGTIHDDQLRRDFTINALAISLNAGSMGALVDPFGGLLDLDRGLLRTPLDPDITFSDD
                     PLRMMRAVRFATQLGFTIDPSTFTAIGRNAQRLDIISAERIHTELNKIILSSRPSIGF
                     ILLRDTGLLAHFFPEFLELQGAEEKYGIGHKDNFYHTLQVLDNVCAASDDLWLRWAAV
                     LHDIAKPRTKRFDPEHGWTFHGHEDKGARMVPGIFRRLKLPLDDQMRFVQKLVALHLR
                     PIALTKDEVTDSAVRRLLFDAGDDIDALMILCRADITSKNERKKDRYLRNYDLVLRKL
                     AEVEEKDRVRNFQPPITGQDVMMAFGVGPSKLIGDIKTVIKDAILDGHIANDRSQAWQ
                     LMLETGRKLGHPPVAGMETPIAEQPQR"
     gene            1328..1906
                     /locus_tag="PL106_02615"
     CDS             1328..1906
                     /locus_tag="PL106_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31990.1"
                     /translation="MSLYRFRVMIDDASEAFRDIEIRRDQTFLDFHHAIKAAFGFKGE
                     EMASFYVSDEEWGKGAEIPLADMGFGEDGHAPALMADTQIGDHIRGKSQRYVYAYDFL
                     NMWMFLCECIHTGKPAKEQTYPTVVLSVGTPPSEHSRMDPILLPAEEEEDNLREEAEE
                     IYDADEDMGFGEEDAEEGDEFGQGDEFPEEHY"
     gene            1912..2850
                     /gene="miaA"
                     /locus_tag="PL106_02620"
     CDS             1912..2850
                     /gene="miaA"
                     /locus_tag="PL106_02620"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNA31991.1"
                     /translation="MPHADRWRAIGAEHGPLPTLIVIGGPTASGKTHAAASLAHQLGT
                     EVISADSRQFYHAMRIGTARPVADELLGVKHHFLGHLELDDTWSAGVFARLAEPVLQG
                     ILERYGIAVLVGGSGLYIDALCNGLDPLPLSDPAVRNRLQLQLEREGLGTLVERLHTL
                     DPAIWATIDRANPHRVIRALEICLLSDAPASTQRSGPVQRTDIRIVRLAMDLPREELY
                     DRIDARVDRMVADGLVEEAHALLPRRHLNALNTVGYKELFDHFDGSLTLQQAIALIKQ
                     HTRNYAKRQMTWLRRDASWKWLAPTDIDGMLHAVPG"
     gene            3031..>4221
                     /locus_tag="PL106_02625"
     CDS             3031..>4221
                     /locus_tag="PL106_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA31992.1"
                     /translation="MNTWNAAPPTVQPPAGPETPVDDIQRIFRLQQESKDRVGSTSVR
                     ERKARLKALHQAILKHRPAIKQAMYEDYRKHPAEVDLVEIYPVTAELKHARRHLAGWM
                     ADHRVPTPLALLGARSRITYEPKGTVLIISPWNFPVNLTFGPLVSALAAGNTVMIKPS
                     ELTPRTSAEIERIVSGVFPENEVAVIEGGVATSTALLELPFNHIFFTGSPAVGKVVMT
                     AAAKHLASVTLELGGKSPSIVDETADLDIAARRIAWSKFVNNGQICITTDHVFVHRTR
                     MEEFIRKVREQVERFYPGDPVNEGSYCRMVNTGHFERVKGYVEDAVAAGARVRMGGTF
                     DASQNYMAPTLIADVPDDSGLMTNEIFGPVLPVIPYSDVNTVIARINQGQKPLGLYIY
                     SRDRR"
BASE COUNT          854 a         1368 c         1284 g          715 t
ORIGIN      
        1 acatgcggca ggctcagggt gacatgcggc aggctcaggg tgacatgcgg caggcccggg
       61 gcgacataca ggtggaattc gtgggtgcgc gcaaagagag ctacagtcgc aacagccgca
      121 aacccgaagt ggaacccggc accatccatg acgaccagct gcggcgcgac ttcaccatca
      181 acgccctcgc catctcgctc aatgccggca gtatgggcgc cctggtggac cctttcggcg
      241 gattgctcga cctggaccgc ggtctgttgc gcaccccgct cgaccccgac atcaccttca
      301 gcgatgatcc cctgcgcatg atgcgggccg tgcggttcgc aacgcagttg ggcttcacca
      361 tcgatccgtc caccttcacg gccatcggcc gtaacgccca gcgcctggac atcatcagcg
      421 cggaacgcat ccacaccgaa ctcaacaaga tcatcctctc ctccaggccc agcatcggct
      481 tcatcctgct gcgcgatacc ggactgctgg cacatttctt ccccgagttc ctcgaattgc
      541 agggcgccga ggagaaatac ggcatcggtc acaaggacaa tttctaccac accctgcaag
      601 tgctcgacaa cgtgtgcgcc gcgagtgacg acctctggct gcgctgggcc gcggtgctgc
      661 acgatatcgc gaagccccgg accaaacgtt tcgatcccga gcacggctgg accttccatg
      721 gccacgagga caagggcgcg cgcatggtgc ccggcatctt ccgacgtttg aagctgccgc
      781 tggacgacca gatgcgcttc gtgcagaaac tcgtggccct ccacctgcgc cccatcgccc
      841 tcaccaagga tgaggtgacc gacagcgcgg tacggcggct cctcttcgac gctggtgacg
      901 acattgatgc gctgatgatc ctgtgcagag ccgatatcac cagcaagaac gaacgcaaga
      961 aggatcgcta cctgcgcaac tacgacctcg ttctgcgcaa gctcgccgaa gtggaggaga
     1021 aggaccgcgt gcgcaacttc cagccaccca ttaccgggca ggacgtgatg atggccttcg
     1081 gcgtcgggcc cagcaagctc atcggcgata tcaagaccgt gatcaaggac gccatcctcg
     1141 acggccacat cgccaacgac cgctcgcagg cctggcaact gatgctggag acaggccgca
     1201 aactcggcca cccgcccgtg gccgggatgg aaaccccgat cgccgaacaa ccgcaacgct
     1261 gaacccgccc gcaccgcggc gcttcgtaca gtagcttcgc actcccgagc cccatcggac
     1321 cacactcatg tccctctacc gcttccgcgt gatgatcgac gatgccagcg aggccttccg
     1381 cgacatcgag atccgccgcg atcagacctt cctcgacttc caccacgcga tcaaggccgc
     1441 cttcggcttc aaaggcgagg agatggcgag tttctatgtg agcgacgagg aatggggcaa
     1501 gggcgccgaa atacctctgg ccgacatggg gttcggcgag gacggacacg ctcccgcgct
     1561 catggcagat acccagatcg gtgatcatat ccggggcaag agccagcgct acgtgtacgc
     1621 ctatgacttc ctgaacatgt ggatgttcct ctgcgagtgc atccacacgg gcaagcccgc
     1681 caaggagcag acctacccga cggtggtact cagcgtgggc acaccgccca gcgaacacag
     1741 tcgcatggat cccatcctgt tgcctgctga ggaggaggag gacaacctgc gcgaggaggc
     1801 cgaggagatc tacgatgccg acgaggacat gggcttcggg gaggaggatg ccgaagaagg
     1861 tgacgagttc gggcagggtg atgagtttcc cgaagaacac tactgagcgc catgcctcac
     1921 gctgatcgat ggagggccat cggcgcggag cacggaccgc ttcccaccct gatcgtgatc
     1981 ggcgggccta cggccagcgg gaaaacgcat gcggccgcct ccctggcaca ccagctgggc
     2041 accgaggtca tcagtgccga ttcgcggcag ttctaccatg cgatgaggat cggtaccgca
     2101 cggcccgtcg cggatgagct gctcggtgtg aagcatcatt tccttggcca tcttgaattg
     2161 gacgacacgt ggagcgcagg tgtgttcgca cggctggcgg aacccgttct ccaaggcatt
     2221 ctggaacgct acggcatcgc ggtgcttgtc ggtgggagcg gcttgtacat cgatgcgctg
     2281 tgcaatggcc tggatcctct gcccctgagc gatccggctg ttcgtaatcg cttgcagctg
     2341 cagctggaac gggaggggct gggcacgctg gtcgagcggt tgcacacctt ggacccggcg
     2401 atctgggcca ccatcgaccg ggccaacccc caccgcgtga tccgcgcatt ggaaatatgc
     2461 ctgctgtccg atgcgccggc cagcacacag cgcagtggcc ccgtacaacg caccgatatc
     2521 cggatcgtgc gactggccat ggacctccct cgggaggaac tgtacgaccg gatcgatgcc
     2581 cgcgtggacc gcatggtggc cgatggcctg gtggaagaag cgcacgccct cctgccccgc
     2641 cgccacctga acgcgttgaa caccgtgggc tacaaggagc tcttcgacca tttcgatggg
     2701 tcactcacct tgcaacaagc gatcgcgctg atcaaacagc acacgcgcaa ctatgccaag
     2761 cgacaaatga cctggctgcg acgggatgcg agctggaagt ggctcgcacc aaccgacatc
     2821 gatggcatgc tgcacgcggt cccggggtga ggtcaccttc aacaccgttg cacggccctc
     2881 ctccttcgta ctccattcat gccgccgctt ataccatcta tgtttgcggt gcgcggaacc
     2941 cggcctcacc cgccattggc acacacttct ccgttcgagg tacgtgatac ggatggactg
     3001 caggccgcat agacacggag aagcatcgtc atgaatacat ggaacgctgc tccgcccact
     3061 gttcaaccgc ccgctggtcc agaaacaccg gtggatgata tccagcggat ctttcgtctc
     3121 caacaagagt ccaaagaccg ggtcggcagt acctccgtgc gggagcgcaa ggcccgcttg
     3181 aaagcgctcc accaggccat cctgaagcac cgccccgcga tcaagcaggc gatgtacgag
     3241 gactacagga agcaccccgc ggaggtggac ctggtggaga tctacccggt gaccgctgag
     3301 ttgaagcacg cacggcggca cttggccggg tggatggccg accaccgggt gcccactccc
     3361 ctcgccctgc tgggcgcccg atcgcgtatc acgtatgaac ccaaaggaac ggtattgatc
     3421 atatcaccgt ggaacttccc ggtgaacctg accttcggcc cgctggtcag cgcgctggcg
     3481 gcgggaaaca ccgtgatgat aaagccctcg gaactcaccc cccgtacatc cgctgagatc
     3541 gagcggatcg tgagcggggt cttcccggag aatgaggtgg ccgtgatcga gggcggtgtg
     3601 gccacttcga cggctctcct ggaactgccc ttcaaccaca ttttcttcac cggttctcca
     3661 gcagtgggca aggtggtgat gaccgctgcc gccaagcacc tggcttcggt aaccttggaa
     3721 ctgggcggca agagcccctc catcgtggat gagaccgcgg acctggacat cgctgcccgg
     3781 cgcatcgcgt ggagcaagtt cgtgaacaat ggccagatct gcatcaccac ggatcatgtg
     3841 ttcgtccacc ggaccaggat ggaggagttc atccggaagg tgcgggaaca ggtggaacgg
     3901 ttctaccccg gtgatcccgt gaacgaagga tcgtactgcc gcatggtgaa caccgggcac
     3961 ttcgagcggg tgaagggcta cgtggaggat gccgtggccg ccggggccag ggtgcggatg
     4021 ggcggcacct tcgacgcgtc gcagaactac atggcgccca ccttgatcgc cgatgtgccg
     4081 gatgacagcg gcttgatgac gaacgagatc ttcggcccgg tgctgcccgt gatcccgtac
     4141 tccgacgtca acaccgtgat cgcacggatc aaccagggtc agaagccgct ggggctgtac
     4201 atctacagcc gcgaccggcg a
//
LOCUS       DAOQOC010000103         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9446_length_4203_cov_3.661765, whole genome shotgun sequence.
ACCESSION   DAOQOC010000103 DAOQOC010000000
VERSION     DAOQOC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_4203_cov_3.661765"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PL106_02630"
     CDS             complement(<1..515)
                     /locus_tag="PL106_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31993.1"
                     /translation="MLSKRKLVNALFVAGFPVYGIGTYLMFDPAMGWSLGLLFASSPF
                     TAIILFHLVDLLYRRRFAVHVGPLFWLCCAVILSFDASVLMGLHYRSPVLIPANAVAI
                     ILQCTVPYLAAVIVQVYNRDVDGFDWSSMLMKCLFALIGINLLGMGAGLHNKLHSFEG
                     RASFPFVLGIY"
     gene            complement(533..1528)
                     /locus_tag="PL106_02635"
     CDS             complement(533..1528)
                     /locus_tag="PL106_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31994.1"
                     /translation="MSEQPYTMRTAVLFIAFRRVEESTAVFQAIREARPPRLYFAVDA
                     PRPGKPEEAAQVARIRELAGLVDWPCELKTLFNEVNKGVKYGPWNAMKWFFDQEEEGI
                     ILEDDCLPHPTWFRFAEDLLEHHRHDQRIWAIIGNNLMDEWMPANDDSYYYTAHGYGA
                     YWGWASWRRTWERYDLDMKDWPELRKSGLLDDHCLSNNERIEANAIFESSWSGHIVGW
                     DFQFDHSRTINGAMNIIPNVNLIRNIGFGNDGTNTLSGLDPRNKDGRQAMGFPLKHPK
                     FFLVDRDRDLAYFSKYIEPSRLRKFKDLIKSLLPAEVDKAVTPFLGKLQRKLGLH"
     gene            complement(1525..2841)
                     /locus_tag="PL106_02640"
     CDS             complement(1525..2841)
                     /locus_tag="PL106_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31995.1"
                     /translation="MNPTSERTWRIDADHGPGPVLVNRDLVPLRPALFNNFNCEHCGA
                     PGPAPVRDLFIGIQILGEYACGSCGTRFFRDLPVGFQVDLPLAFTTEERRILHWNAEP
                     WITYPEYAVPTDPGPRTERKVYREHDRIIVLNTLDHLYGHVLLKLYNAQHYLDHYKDR
                     GLVVIVPRMFEWLVPKGVAETWVVDQKLSQARAWHPAIDRFVQEQLPRYKEVDLARGW
                     GHPNMAGVDISRFTGVEPFPLDQYVSTAPHITFVARTDRLWFRTTLGKLCYRAIGALG
                     LRNSLGRWFTYRQSAMMRSVMRRVKRALPDASFTVVGLAPSGGFEGLAEDLRTMRMDT
                     GTELQWCRAYARSHIVVGVHGSNMLLPTAHAAGLIEILPRDRFGNFGQDVSIRYRDRM
                     QLFLYRFVDEFADPGTVARNAVSMIRHFTVYHRNNITNTFGLHRTS"
     gene            complement(2849..>4203)
                     /locus_tag="PL106_02645"
     CDS             complement(2849..>4203)
                     /locus_tag="PL106_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNA31996.1"
                     /translation="SSPVVGMLRSEGFQRYFRNTSWLFVDRVVRLVSVLATSFFVVRF
                     LGPELFGELNYASAFVGMFFALTSMGMEDIITRDLVRHPERRDTLLGSAAVLKLGGSV
                     VLFITVLAIALLKGMPAFKVLLVALIASAEFLKPLSVIEPFFHSRTQGRIVAKVNIAQ
                     SIINALFRIGVILVVETRQDAMIPLALSYIVEMATTSGSYLLAYRNQGLNWRAWRFDR
                     VLAIDLLRQSWPLIVFGIALYVQAKIDQVIIGDVLRDQLGEGGADAEVGQYSAALKMI
                     ESLGFLPSIVAASLAPAITRAHMQDRALFERRVTDQYRLMFLLFLVTAIPLYFLAPPF
                     MTWFYGEEFNEAGQLLALFAIRLFFTNMGVAKTAFITNEGLFRYSMITAIVGAVCNIV
                     LNLLLIPGLHAKGAIWAMIISFLISNFLLDLFYREARPNFKWTMHAIATFWRVRHATA
                     "
BASE COUNT          829 a         1273 c         1340 g          761 t
ORIGIN      
        1 tcgtaaatgc cgagcacgaa ggggaaactc gcccgcccct cgaaactgtg cagtttgttg
       61 tgaagcccgg cccccatgcc cagcaggttg atgccgatga gcgcgaagag gcacttcatc
      121 agcatggagg accagtcaaa gccgtccaca tcacggttgt acacctgtac gatcaccgcg
      181 gccaggtatg gtacggtgca ttgaaggatg atggccacgg cgttcgccgg tatgagcacc
      241 gggctgcggt aatgcaggcc catgagcacc gacgcgtcga atgagaggat caccgcgcag
      301 cacaaccaga aaagcggccc cacatgcacc gcgaacctcc tccggtacag caggtccacc
      361 aggtggaaaa ggatgatcgc ggtgaagggc gaactggcga acaacagacc gaggctccat
      421 cccatcgcgg gatcgaacat gagatacgta ccgatgccgt aaaccgggaa acccgccacg
      481 aagagggcgt tcaccagttt tcgtttggag agcacggctc gggatccagg gttcagtgca
      541 gacccagctt gcgctgcagc ttgccgagga agggggttac cgccttgtcc acttcagcgg
      601 gcagcaggct cttgatcagg tccttgaact tgcgcaggcg gctgggttcg atgtacttcg
      661 agaaataggc cagatcgcga tccctgtcca cgaggaaaaa tttcgggtgt ttcagcggga
      721 atcccatcgc ctggcggcca tccttgttcc gggggtccag cccgctcagc gtgttggtcc
      781 cgtcgttccc gaagccgatg ttgcggatca ggttcacgtt cgggatgatg ttcatcgcac
      841 cgttgatcgt gcggctgtga tcgaactgga agtcccagcc cacgatatgg ccgctccatg
      901 agctctcgaa gatcgcgttg gcctcgatcc gttcattgtt ggagaggcag tggtcgtcca
      961 ggagaccgct cttccgcagc tcaggccagt ccttcatgtc caggtcgtac cgttcccatg
     1021 tacggcgcca actggcccat ccccagtacg cgccgtatcc gtgcgcggtg tagtagtacg
     1081 aatcgtcgtt cgcgggcatc cattcgtcca tcaggttgtt cccgatgatc gcccagatcc
     1141 gttggtcgtg ccggtggtgt tccaacaggt cctccgcgaa gcggaaccat gtggggtgcg
     1201 gcaggcagtc gtcctccagg atgatcccct cctcctcctg gtcgaagaac cacttcatcg
     1261 cgttccacgg accgtacttc acgcccttgt tcacctcgtt gaacagggtc ttcaattcgc
     1321 agggccagtc caccagcccg gccagctcac ggatgcgggc aacttgggcg gcttcttcgg
     1381 gcttgccggg gcgaggcgcg tccacagcga agtaaagccg tggcgggcgg gcctcacgga
     1441 tcgcctggaa caccgccgtg ctctcttcca ctcgccggaa ggcgatgaag agcaccgcgg
     1501 tgcgcatcgt gtacggctgc tcgctcatga tgtgcggtgg agcccgaagg tattggtgat
     1561 gttgttgcgg tggtacaccg tgaaatgcct gatcatcgac accgcgttgc gcgcaacggt
     1621 tcccgggtcc gcgaactcgt ccacgaaccg gtacaggaag agttgcatgc ggtcgcgata
     1681 gcggatgctc acgtcctggc cgaagttgcc gaagcggtct cgcggcagga tctcgatgag
     1741 gcccgctgca tgcgccgtgg gcagcagcat gttgctgccg tgcacgccca ccacgatgtg
     1801 gctccgggca taggcacggc accattgcaa ttcggtcccc gtgtccatgc gcatggtacg
     1861 caggtcctcg gcaaggccct cgaagccgcc cgatggcgcc agacccacta ccgtgaatga
     1921 ggcatcgggc agggcccgct tcacccggcg catcacgctg cgcatcatgg ccgattgccg
     1981 ataggtgaac cagcgcccca gactgttcct cagccccagg gcaccgatcg cccggtagca
     2041 caacttgccc agggtcgtgc ggaaccagag ccggtccgtg cgggccacga aggtgatgtg
     2101 cggagcggtg gatacatact ggtcgagcgg gaacggctcc acgccggtga agcggctgat
     2161 atcaacgccc gccatgttcg gatgccccca accccgcgcc aggtccacct ccttgtaccg
     2221 cggcaactgc tcctgcacga agcgatcgat cgcggggtgc caggcgcggg cctgcgagag
     2281 tttctggtcc accacccagg tctcggccac gcccttcggc accagccatt cgaacatccg
     2341 tggcacgatc accaccaggc cccggtcctt gtaatggtcc agatagtgct gcgcgttgta
     2401 cagcttcaac agcacatggc cgtacaaatg gtccagcgta ttgagtacga tgatccggtc
     2461 atgctcccgg tacaccttgc gttcggtacg cggaccggga tcggtcggga cggcgtactc
     2521 cggataggtg atccacggtt cggcgttcca gtgcaggatg cgtctctcct ccgtggtgaa
     2581 ggccagtggc aggtccactt ggaaaccgac gggcaggtcg cggaagaaac gtgtgccaca
     2641 ggaaccgcag gcgtattcgc cgagtatctg gatcccgatg aagaggtcgc gcacgggcgc
     2701 gggtcccggt gccccgcagt gctcgcagtt gaagttgttg aagagggcgg gacgcaaggg
     2761 caccagatcc ctgttgacca gtacgggccc cggtccgtga tcggcgtcga tgcgccatgt
     2821 ccgttcgctg gtcgggttca tcgctcgatc atgcggtggc atgccgcaca cgccagaagg
     2881 tggctatggc gtgcatggtc catttgaagt tgggccgggc ctcgcggtag aacaggtcca
     2941 gcaggaagtt gctgatgagg aaactgatga tcatggccca gatggccccc ttggcgtgca
     3001 ggcccggtat caacaagagg ttgagcacga tgttgcacac ggcaccgacg atcgcggtga
     3061 tcatcgagta gcggaacagc ccctcgttgg tgatgaaggc ggttttcgcc acgcccatgt
     3121 tggtgaagaa caggcggatg gcgaagaggg cgagcagttg ccccgcctcg ttgaattcct
     3181 ccccgtagaa ccatgtcatg aacggcgggg cgaggaagta aaggggaatg gcggtcacca
     3241 ggaagagcag gaacatcagc cggtattgat cggtgacgcg ccgctcgaaa agtgcccggt
     3301 cctgcatgtg ggcccgggtg atcgccgggg cgagactggc cgccacgata ctgggcagga
     3361 agccgagcga ttcgatcatt ttgagcgcgg ccgagtattg gcccacctcg gcatcggcac
     3421 cgccttcgcc caactggtcg cgcagcacgt cgccgatgat cacctggtcg atcttggctt
     3481 gcacgtacaa agcgatgccg aacacgatca acggccagga ttggcgcagc aggtcgatgg
     3541 ccagtacacg atcgaaccgc catgcccgcc agttgagccc ctggttcctg tatgccagga
     3601 ggtaactgcc ggaggtggtc gccatttcaa cgatgtacga cagggccaga gggatcatgg
     3661 cgtcctgccg tgtctccacc accaggatca caccgatccg gaacagggcg ttgatgatcg
     3721 attgcgcgat gttcaccttg gccacgatgc ggccctgcgt ccgcgagtgg aagaagggct
     3781 cgatcaccga gagtggcttg aggaattcgg cgctcgcgat gagcgccacc aacagtacct
     3841 tgaaggcggg catgcctttg agcagtgcga tggcgagcac agtgatgaac agcaccaccg
     3901 aaccgcccag cttcagtacc gcggccgacc ccagcagggt atcccggcgc tcaggatgcc
     3961 gcaccaggtc gcgtgtgatg atgtcctcca tgcccatgga ggtgagggcg aagaacatgc
     4021 ccacgaacgc actcgcatag ttgagctcgc cgaagagttc ggggcccagg aagcggacga
     4081 cgaagaaact ggtcgcgagg acggagacca gccgcacaac acggtcaacg aacagccacg
     4141 atgtgttgcg gaagtagcgt tggaagcctt cggatcggag catcccgacg accggactac
     4201 ttg
//
LOCUS       DAOQOC010000104         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9579_length_4167_cov_3.217899, whole genome shotgun sequence.
ACCESSION   DAOQOC010000104 DAOQOC010000000
VERSION     DAOQOC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9579_length_4167_cov_3.217899"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..464
                     /locus_tag="PL106_02650"
                     /pseudo
     CDS             78..464
                     /locus_tag="PL106_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
     gene            complement(900..1199)
                     /locus_tag="PL106_02655"
     CDS             complement(900..1199)
                     /locus_tag="PL106_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA31997.1"
                     /translation="MKNSLIGIRVDHRQYGLIVVKSSSPAIRKMTVRMVENPVQPLAL
                     RLAAWNSPLIASSDPLVRRDRAHAMMPFGWFPIMLATDRIGSTFDHRTLTHQWLV"
     gene            1351..1761
                     /gene="rnk"
                     /locus_tag="PL106_02660"
     CDS             1351..1761
                     /gene="rnk"
                     /locus_tag="PL106_02660"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNA31998.1"
                     /translation="MGKLIVNRLDQARIKKCINEAMRIKSIGKAEAEKLLMELDSAEI
                     VDPESIPANVVTMNSIVKLSFLNTDKRIQFQLVYPEQANIKENKISIFSPIATALIGY
                     KVKDEIEWIVPAGLTKIRIDEIVYQPEATGDYDL"
     gene            complement(1883..2956)
                     /locus_tag="PL106_02665"
     CDS             complement(1883..2956)
                     /locus_tag="PL106_02665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNA31999.1"
                     /translation="MTTGTSDLVRTLCEAFGITPVEHDKIPYGSGLINKTFLLKDRVD
                     GNHWLLQQVNTTVFKRPELIAFNNYLAAEHLRSHHPDYLFMRGRRTAHGKDLLIHPEL
                     GTWRVFPYFANTISYDQAPTPAHAFSAAQQFGRLTRKLDGVYVGAFQAVIPGFHDLSV
                     RFTEFKASIPVATEERRQQAQPVIDLILDRAHIVDEFNRAMADPDVRTRITHNDTKLN
                     NVLFDKDSGEAVCVVDLDTLMPGKAIFDLGDMIRTFISTAAEDDPDPAHIDINADVFE
                     QLVRGWTSEMAPLLSASERALLYWSGQLLMYEQCIRFLTDLLQGDVYYRTQYPGHNLD
                     RAKNQMHLLNAYEERREELEARL"
     gene            complement(2953..3729)
                     /locus_tag="PL106_02670"
     CDS             complement(2953..3729)
                     /locus_tag="PL106_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNA32000.1"
                     /translation="MPALILLAGGKSTRFGRPKQLEPVGPRGEALLDLTLRDAFAAGC
                     TDAVIVVRPEHGTEFAERFVEDRRVRIIVQHEARGTAHAVMLALDHVHGTAITANGDD
                     HYGKEALQLAVDHALHADPQVHALVAFHLANTLSPGGGVNRAVCTTDIHGLLLFTEEV
                     TGLHAGPDGISGPDGRTWPPDTMVSMNLWVFRPALFPLFRTLYAAHVPTNGEFGLPNV
                     VADAIAQQHRFVLVRTAAQWCGLTHPADAEIVRRELSRQP"
BASE COUNT          811 a         1187 c         1342 g          827 t
ORIGIN      
        1 aatactctcg tgggctgact accgctcaag cggatcagtt tgtggggatg tatgtgaatc
       61 atcacaccat taaagccagc gctgatgtga agcgtgccgt gcgcttgatg ctggccatgg
      121 gggccgacgt gtgcaacagt gctcgcggtt tcctgttctc cctgggttgt atccaggctt
      181 tgcgctgcaa caccaatgac tgccccaccg ggatcaccac gcaccaacgc ggcctggtgc
      241 gcggtctggt ggtgaccgag aagagcgagc gtgtgtacca cttccaccgc aacaccctgc
      301 attcgttgat ggaactgctg gcggcttgcg ggttggacca tgtggagcag gtgaacaccg
      361 gtcatttcat gcgcggtgac gagttcgtga agctggccga tcgctacttc ccggatggcc
      421 tgggccacgt gatgcatccg tcgccgagcg atagcaaggg atagtgcggg caggtggtcg
      481 ggccgccgtt ggggcaggat ggatccacgg atcggtccgg tgttccacga cttgtcctga
      541 tcggatccgg gccacggatg gacaggtaac ccgcccatga cgtcgcaatt ccatggagcc
      601 cgggtgcggc catatccatg ttcgtttgcc ggtagcatta cccatcctgg tggaagccgg
      661 gctcgtggcg ggcaattcga tccttggacc tggaactggc atcccgtgcc cctgccggtg
      721 atggccggtt gctggcatca gtgcctacca acatggcgat ccccaagaag tacgttgatc
      781 gatgcgtgga gagtccggac cacagcgagg ggaccgtgcg ctcaagggca tgcactggaa
      841 gctggtgcga aaccccgagg acctcgacca ggagtcgcgt gccgagctgg atagcttcat
      901 cagacaagcc actggtgtgt caacgtacgg tgatcgaagg tggagccgat gcggtcggta
      961 gccaacatga tcgggaacca tccgaatggc atcatcgcat gggcgcggtc acggcgaacc
     1021 aacggatcgc tcgaagcgat caacgggctg ttccaagccg ccaggcgcag agccagaggc
     1081 tgcaccgggt tctctaccat acgtaccgtc atcttcctga tcgccggtga actggacttc
     1141 accacgatca acccatactg ccggtgatcc actcgaattc caataaggct gttcttcatc
     1201 cccatcatcc cgcgcattcg aacgcatggc gtgctgtccg ggtcggaacc gcatccgtat
     1261 gctttatttt cagcggaccg ccccattgaa ttcggcacgg atcatcacct ttgcacccca
     1321 gacccccgaa cgggcaagaa ctgaacgaac atgggcaaat tgatcgtgaa ccgattggat
     1381 caagcgcgga tcaagaagtg catcaacgaa gcgatgcgga tcaagtcgat cggcaaggcg
     1441 gaggcggaga aactgttgat ggaattggat tcggcggaga tcgtcgatcc cgagtccatc
     1501 ccggccaacg tggtcacgat gaactcgatc gtgaagttga gctttttgaa caccgacaag
     1561 cggatccagt tccaattggt ctatccggaa caggcgaata tcaaggagaa caagatctcc
     1621 atcttttcgc cgatcgccac ggcgctgatc ggatacaagg tcaaggacga gatcgagtgg
     1681 atcgttccgg ccggattgac gaagatcagg atcgacgaga tcgtgtacca gccggaagcc
     1741 actggggact atgatctgtg agagatcgtc cggaggcatg cgtggcgtgg tggccgtgga
     1801 ggaacaaccc gatcgttgac ggggcctcgc ccgttgttcc tcaccacctt ccgtagatgg
     1861 ccgggcaggg cccctgcgtc gttcacagcc gagcttccaa ctcctccctc cgttcctcgt
     1921 acgcattcaa caggtgcatc tggttcttcg cgcggtccag gttgtggccg gggtattggg
     1981 tgcggtagta cacgtcgcct tgcaggagat cggtgaggaa acggatgcac tgttcataca
     2041 tgagcaattg cccgctccag tacagcagcg cacgctcact ggccgagagc aagggcgcca
     2101 tctcactggt ccagccgcgc acgagctgtt caaagacgtc cgcattgata tcgatgtgcg
     2161 cggggtccgg gtcgtcctcg gccgcggtgg agatgaaggt gcggatcatg tcgcccagat
     2221 cgaagatggc cttgccgggc atcagcgtat cgaggtccac cacgcacacg gcctctccac
     2281 tgtccttgtc gaagagcaca ttgttcagtt tggtatcatt atgcgtgatg cgggtccgca
     2341 cgtcagggtc cgccatggcc cggttgaact cgtccacgat gtgggcccgg tccaggatga
     2401 ggtcgatcac gggctgcgcc tgttgacgtc gttcttcggt ggcgacgggg atgctcgcct
     2461 tgaattccgt gaagcgcacc gacaagtcgt ggaacccggg gatgacggcc tggaaagcgc
     2521 ccacgtagac gccatcgagc ttccgggtga gtcggccgaa ctgctgtgcg gcggagaagg
     2581 catgggccgg ggtgggcgcc tggtcgtagc tgatggtgtt ggcgaagtag gggaatacgc
     2641 gccatgtgcc cagttcggga tggatcagca ggtccttgcc atgcgccgtg cggcggccgc
     2701 gcatgaagag gtagtccggg tggtgcgagc gcaggtgttc ggcggccagg tagttgttga
     2761 aggcgatcaa ttcgggccgc ttgaacacgg tggtgttcac ctgttgcagc agccagtggt
     2821 tgccgtccac acggtccttc agcaggaatg tcttgttgat gagaccactg ccgtaaggga
     2881 tcttatcgtg ctccacgggg gtgatgccga atgcttcgca aagcgttcgc acgaggtcgc
     2941 tggttccggt ggtcatggct gtcgggagag ttcacgacgc acgatttcgg cgtctgccgg
     3001 gtgggtgagg ccgcaccact gcgccgcggt acgcacaagg acgaagcggt gctgttgcgc
     3061 gatggcatcg gccaccacgt tgggcagtcc gaactcgccg ttggtgggga cgtgcgccgc
     3121 gtacagcgtg cggaacaagg ggaagagcgc ggggcggaac acccacaagt tcatgctcac
     3181 catcgtatcc gggggccacg tgcggccatc gggtcccgaa atgccgtcgg gaccggcgtg
     3241 cagaccggtc acctcttccg tgaagagaag taatccgtga atatcggtag tgcatacggc
     3301 acggttcaca ccaccaccgg ggctcagggt gttcgccaga tggaaggcca ccagggcgtg
     3361 tacctgcgga tcggcatgca gggcatggtc cacggccagt tgaagggcct ccttcccgta
     3421 atgatcgtcg ccgttcgcgg tgatcgcggt accatgcaca tgatccagtg cgagcatcac
     3481 ggcgtgcgcg gtgccgcgtg cttcgtgctg tacgatgatg cgcacacgtc ggtcctccac
     3541 gaagcgttcg gcgaactccg ttccatgctc tggccgcacc acgatcaccg catcggtgca
     3601 tccggcagcg aaggcatcac gcagtgtgag gtccaacaag gcttccccac gcggccccac
     3661 gggctccaat tgcttggggc gcccgaagcg ggtgctcttg ccgccggcga gaaggatgag
     3721 ggcaggcatg ggagagggaa aggtagcgaa gagcgctcaa ccggcatcga tcaagcccga
     3781 acgcaccttg cgccatcgcc atggtgatcc ggagttgatg gatggtccgg ccatgcggaa
     3841 gccccagggc tatcgcgtca tatcgtccat gtcgacgcac ccgcgttgtg cgacactctt
     3901 cggggtcaat agctgcactg gatacgggtt ctacgctgtg tgacccactt caggtcacgt
     3961 tcagtcttgc ttcggttgac cccagagagg tcatataacg tcgcactttc ttgacagacg
     4021 acggttcgac cctgaagggg tcgcacaatt ttcccttgtc tgccggaatc ttactgagcc
     4081 ggtgatgaag atccggtctc tcgatcatcg cggtcagaat gaaccatggg ggtgcgaaag
     4141 tgcgagggtg cgagagtcac gtctctc
//
LOCUS       DAOQOC010000105         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9612_length_4158_cov_3.525957, whole genome shotgun sequence.
ACCESSION   DAOQOC010000105 DAOQOC010000000
VERSION     DAOQOC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9612_length_4158_cov_3.525957"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1057)
                     /locus_tag="PL106_02675"
     CDS             complement(242..1057)
                     /locus_tag="PL106_02675"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNA32001.1"
                     /translation="MARKTAFALLGACSLSIGFAQGRTQENNPPSGANSLRTIEHNAF
                     KAGEKLEYVLHYGLMNAGVATLELKEGGLDILGRKTLHAHGEGHSTGAFKAFYTVNDV
                     YDSRFDKDGVFPWIFTRRVDEGGYLINQDYTFMQHGRKVNTQDNKSFDAPAHVQDMIS
                     AFYYARTWDLRNVQAGDEFTMDLFMDNENWPLKMRFIGRETIKIRNGKYRCLKFQPMV
                     QKGRIFKSNDDLNVWISDDPNHVPILVQAKILVGSIKMELSGYEGLANPIAKE"
     gene            complement(1117..3921)
                     /gene="leuS"
                     /locus_tag="PL106_02680"
     CDS             complement(1117..3921)
                     /gene="leuS"
                     /locus_tag="PL106_02680"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNA32002.1"
                     /translation="MAYEHKDIEKKWQEEWRKRGTYRVNNDPSRPGYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIVARYKRHCGFNVLHPMGYDSFGLPAEQYAIQTGQHPAKTTAE
                     NAARYRQQLDAIGFSFDWDREVRTSDPAFYKWTQWIFLQLFDSWYDPKTDKARPISEL
                     VACFQEKGSIGQDTSTLTGDIEEFVGGFTAEEWRSFDERTRQLVLQHFRLAYISDAWV
                     NWCPAMGTVLANDEVKDGVSERGGHPVERKRMKQWSLRITSYAQRLLDGLDTLDWSES
                     IKEAQRNWIGRSEGATVRFPIGNDFVEVFTTRPDTLFGVCFITLAPEHVLVGKFTTPE
                     RKAEVEAYVEIAKNRSERDRMADVAHVSGVFTGSFAKHPFTGADVPVWVGDYVLAGYG
                     TGAVMAVPGGDQRDWRFAKHFGLPIIAVTEGADIEKEADERKDATICSDGFLKGLKVP
                     QAIARAIEELERSGAGQGRINFRLRDAAFGRQRYWGEPIPIYYKDDIPYALPESELPL
                     ELPAVDKFLPTAEGEPPLARATDWTYKGMPLETTTMPGWAGSSWYFLRYMDPGNNDRF
                     TSPEAINYWKQVDLYVGGSEHATGHLLYFRFWTKFLFDRGWIPFDEPAKKLVNQGMIQ
                     GISMMARVAVDRSNGVERYLVLPKEKHDTPGAFDRNYLIDQVNFKEGRYFINEEGAAR
                     TRAKEAIFREFHLGAPEVELTPVVEKMSKSKFNVVNPDDIIAKYGADTLRLYEMFLGP
                     LEQSKPWDTNGIEGTFRFLRKFWNLFHDPNDAFHVREAEPTKPELKILHATLKKVSED
                     IGKLSFNTSVAQFMIAVNELGTLKCDKRAILEPLTIALAPFAPHIAEELWQRLGHAES
                     ITTATWPTWDEQYLVEDSFSYPISFNGKTRLQLEFPIALSKEEVEAQVLANPEVQLRL
                     EGKAPKKVIVVPKRIVNIVV"
BASE COUNT          695 a         1258 c         1311 g          894 t
ORIGIN      
        1 tcactgtagc cacatagctg gagcagggta cgatgatgtt gttcgcagtt aacgtgcttg
       61 ccgaaatgcc gacctgggca tgtgttgcga aacccagtag ggaacaggct gccgccgtga
      121 tgtggactcg gaagtgcatg gtcatcgatc tggttccggt cagtcacgac cgtgccacaa
      181 agacaaccac gtcaagcgga ctattcctac atcagaactg atggatcatg gaagttcgcg
      241 actattcctt cgcgatcggg ttcgccagcc cctcgtaccc gctcagttcc atcttgatgg
      301 agcccaccag gatcttcgcc tgcaccagga tcggcacgtg gttcgggtcg tcgctgatcc
      361 acacgttgag gtcgtcgttg ctcttgaaga tgcggccctt ctgcaccatg ggctggaact
      421 tcaggcagcg atacttgcca ttgcgtattt tgatggtctc acgcccgatg aagcgcatct
      481 tgagcggcca gttctcgttg tccatgaaca ggtccatggt gaactcgtcg cccgcttgca
      541 cattgcgcag gtcccaggtg cgtgcgtagt agaaggcgct gatcatgtcc tgcacgtgcg
      601 cgggcgcgtc gaagctcttg ttgtcctgcg tgttcacctt gcggccgtgt tgcatgaagg
      661 tgtagtcctg gttgatgagg tagccgccct cgtccacccg gcgggtgaag atccaaggga
      721 agacgccgtc cttgtcgaag cgcgagtcgt agacgtcgtt caccgtgtag aaggctttga
      781 aggcgcctgt gctgtggcct tcgccgtgcg cgtgcagcgt cttgcggccc aggatgtcca
      841 gaccgccttc cttcagttca agggtggcca caccggcgtt catcaggccg tagtgcagca
      901 cgtactccag cttttcgccc gccttgaagg cgttgtgctc gatggtgcgc agggagttcg
      961 cgccagaagg cgggttgttc tcctgcgtgc gaccctgggc gaagccgatg gaaagggaac
     1021 atgcgcccag cagtgcgaat gcggttttac gtgccatgtc cgcccagttg caaggagcgt
     1081 accggagtaa catgaccgct cgtggccggg gcttcatcag accacgatgt tcacgatgcg
     1141 cttgggcacc acgatcacct tcttgggcgc cttgccttcg agccgcagct gcacctcggg
     1201 gttggccagc acttgcgctt ccacctcttc cttgctcagg gcgatgggga actccaattg
     1261 caggcgcgtc ttcccgttga agctgatcgg gtagctgaag ctgtcctcca ccaggtattg
     1321 ttcgtcccat gtgggccagg tggcggtggt gatgctttcg gcatgcccca gtcgctgcca
     1381 gagttcttcg gcgatgtgtg gcgcgaaagg ggccaaggcg atggtgagcg gttcgaggat
     1441 ggcccgcttg tcgcacttca aggtccccaa ttcgttcacc gcgatcatga actgcgccac
     1501 gctggtgttg aagctcagct tcccgatgtc ctcgctcacc ttcttcaggg tggcgtgcag
     1561 gatcttcagt tcgggcttcg tcggttccgc ttcgcgtacg tggaacgcat cgttcggatc
     1621 gtggaaaaga ttccagaatt tgcgcaggaa gcggaaggtg ccctcgatgc cgttcgtgtc
     1681 ccaaggcttg ctctgttcca acgggcccag gaacatctcg tacagccgca gggtgtcggc
     1741 gccatacttg gcgatgatgt cgtccgggtt caccacgttg aacttcgact tggacatctt
     1801 ctcgaccacg ggtgtgagtt ccacctcggg ggcgcccaga tggaattcgc ggaagatggc
     1861 ctctttcgca cgggttcgtg ccgcaccttc ctcgttgata aaatacctgc cctccttgaa
     1921 gttgacctgg tcgatcaggt agttccggtc gaaggcgccg ggggtatcgt gcttctcttt
     1981 tggcaacacg aggtaccgct ccaccccgtt actgcggtcc acggccaccc tggccatcat
     2041 gctgatgccc tggatcatcc cctggttcac caatttctta gcgggttcat caaaggggat
     2101 ccagccgcga tcgaacagga acttggtcca gaagcggaag tacaacaaat gacccgtggc
     2161 gtgctcgctg ccgcccacgt acaggtccac ctgtttccag tagttgatgg cttccggtga
     2221 ggtgaaacgg tcgttgttgc cgggatccat gtagcgcagg aagtaccagc tgctgcccgc
     2281 ccagccgggc atggtggtgg tttccagcgg catgcccttg taggtccagt ccgtggcacg
     2341 tgcgagtggc ggctctcctt cggccgtggg caggaacttg tccacggcgg gcagctccag
     2401 tggaagctcg ctttcgggca gggcgtaggg gatgtcgtcc ttgtagtaga tggggattgg
     2461 ctcaccccag tagcgttggc ggccgaaggc ggcatcgcga agccggaagt tgatgcggcc
     2521 ctgcccggca ccggagcgtt ccagttcttc aatggcgcgg gcaatggcct gcggcacctt
     2581 caggcccttg aggaagccat cgctgcagat ggtggcatcc ttgcgctcgt cggcctcctt
     2641 ctcgatatcg gcgccttcgg tcacggcgat gatgggcagg ccgaagtgct tggcgaagcg
     2701 ccagtcgcgt tgatcaccgc cgggcacggc catcaccgcg ccggtgccgt agccggccag
     2761 cacgtaatcg cccacccaca cgggcacatc ggcgccggtg aacggatgct tcgcgaagct
     2821 accggtgaac acgccgctca catgggccac atcggccatg cgatcacgtt cgctgcggtt
     2881 cttggcgatt tccacgtagg cttccacctc ggccttgcgc tcgggggtgg tgaacttgcc
     2941 caccagcaca tgttcgggag ccagggtgat gaaacatacg ccgaacaagg tatctgggcg
     3001 cgtcgtgaac acctcgacga agtcgttgcc gatgggaaaa cgcaccgtgg ccccttcgct
     3061 gcgaccgatc cagttgcgct gggcctcctt gatgctctcg ctccaatcca gcgtatccag
     3121 cccgtcgagc aggcgctgcg cgtacgaggt gatgcgcagg ctccactgtt tcatgcgttt
     3181 gcgttccacg gggtgcccgc cacgttcgct cacgccatcc ttcacctcgt cgttggccag
     3241 caccgtgccc atggcggggc accagttcac ccaggcatcg ctgatgtacg ccagacggaa
     3301 atgttgaagc accagttgtc gggtgcgttc atcgaacgac cgccattcct cggcggtgaa
     3361 gccccccacg aactcctcga tgtcgcctgt gagggtgctg gtgtcctgtc caatactacc
     3421 tttttcctgg aagcaggcga ccagctcgct gatgggccgg gccttgtcgg tcttggggtc
     3481 gtaccagcta tcgaaaagtt gcaggaagat ccactgcgtc cacttgtaga aggcggggtc
     3541 gctcgtgcgc acctcgcggt cccaatcgaa gctgaagccg atggcatcca actgctgtcg
     3601 gtagcgcgcg gcgttctcgg ccgtggtctt cgcggggtgc tgccccgtct ggatggcgta
     3661 ctgttcggcg ggcaggccga agctgtcgta gcccatgggg tgcagcacgt tgaacccgca
     3721 atgccgcttg tagcgcgcca cgatgtcgct ggcgatgtag cccaaggggt gccccacgtg
     3781 caggcccgcg ccgctggggt aggggaacat gtcgaggacg tagtagccgg gcctggaagg
     3841 gtcgttgttc acgcggtagg tgccgcgctt gcgccattcc tcctgccatt tcttctcgat
     3901 gtccttgtgc tcgtaagcca tgtgcccgtc tggaaggtgg cgaaggtact ggccccttcc
     3961 atcccgtgag gaacgggcgc cgggtggggc gggcatccag cggaactcac aaccgctgac
     4021 tgacaacccg tggacgggcg gccgccaccc cgttcccggg tgcggataga ttcggccccc
     4081 gatgggagtg gatcgccagt tcagggcccg ggcccgcacg agcaacgtgg gcaccatcat
     4141 cggcatcacc ctggtgct
//
LOCUS       DAOQOC010000106         4157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9616_length_4157_cov_4.558020, whole genome shotgun sequence.
ACCESSION   DAOQOC010000106 DAOQOC010000000
VERSION     DAOQOC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_4157_cov_4.558020"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PL106_02685"
     CDS             complement(<1..482)
                     /locus_tag="PL106_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA32003.1"
                     /translation="MEHFDLCVIGGGPAGYAAAMRAIDLGLRTVLVERERIGGTGIFN
                     GALMSKTLWELSQRVTSANEVIRERSRQPFRLSWPEISKVLNEAVFERKYLYACHLQL
                     LENSGDRFKHMRGTAHFTGPHTVMVSRSDARTTVHADHFVIATGSRPRSMPDVRVDER
                     R"
     gene            664..2256
                     /locus_tag="PL106_02690"
     CDS             664..2256
                     /locus_tag="PL106_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32004.1"
                     /translation="MTRQRSSILICTLVATISVSAQTVRSNAYARMLDDAGGLLTAER
                     WEDAAKVYRRLEIMDSTSAEVAYGLGVCLGHIPGQRTAATERFARASRYGHLEAKYQL
                     ALVLHRQQRFDEALALLAYYKSAAEHEVPDAEVDRQVRMVATARELQADPVQVNIANM
                     GPRINSKDNDYSPVTTADGATLYFTSRRKGSTGEARDPSGQYLEDIYMARKEGGEWGS
                     AMNVGVPLNTFVHDATVGLSPDGGSMIIYRTAQNLVSGDLYECVKHLGAWEPPTKMTE
                     RINSSSHEPSATIAPDGSEIYFTSDRPGGMGGRDIYRIRKLPGGEWSLPLNLGPVINT
                     PFDEDAPFMHSDGKTLFFSSNGHATMGGYDIFKSTLTDPDMNGWTAPENMGHPLNTVN
                     DDIFFCLSEDGRTGYFSSERADGLGGQDIYSIVFPESQLEEIVVRGVVTDGMEEPVQA
                     RIVLTGEADGEMVGVYNTNARTGRYLMVVKPGERYNMTVEAPGFVPRSDALSTVGKSG
                     GLTEIPLDIILVRDEKTARVKP"
     gene            complement(2260..3243)
                     /gene="trpS"
                     /locus_tag="PL106_02695"
     CDS             complement(2260..3243)
                     /gene="trpS"
                     /locus_tag="PL106_02695"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNA32005.1"
                     /translation="MPRILTGIQSTGTPHLGNLMGAIVPAIAMGRLPGNDAFLFIADL
                     HSFTQLRDPQLRRENTYAVAAAWIAMGLDLERTTFYRQSDVPEVTELTWYLECLTPCP
                     MLENAHSYKDALAKGSVPNAGVFNYPVLMAADILLYDSEVVPVGKDQKQHLEITRDIA
                     SAFNRAYGEDTFVVPKARIDEQLMTIPGTDGRKMSKSYGNTINVFLPEKELKKQVMGI
                     VSDSRTLEEPKDPGNDNTFKLFALTATPERTTAMRENYLRGGYGYGHAKKELLDVLIT
                     AFAKERTLFHDLMNDRGELDRLLRIGAEKAREVADRTLARVRERAGYGRSA"
     gene            3359..>4157
                     /locus_tag="PL106_02700"
     CDS             3359..>4157
                     /locus_tag="PL106_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA32006.1"
                     /translation="MSMYQHHDQLVVCDGESMRAPVLKWLFVVPRVLFKLWMFLVFFT
                     SLAVLYVPFRLRLRKEEEHPRAFRLMNAWNRFLNVFLLVPVKRVSMAPLPPPPYVICA
                     NHGSYLDILHMYSMGLDFFLFMGKYELLKWPLLGMFFRRMNIAVNRGNRTEAVKALAH
                     AARSIDKGICVALFPEGTIPVSAPRMQRFKDGAFKLAIEKQVPIVPITFLDHWKLFGE
                     PGELCSRGHPGFAHVVRHPFISTAGLTMADKEALRRRAGTVFRVLESG"
BASE COUNT          729 a         1260 c         1389 g          779 t
ORIGIN      
        1 cggcgctcgt cgacacggac gtcgggcatg gatcgcgggc ggcttcccgt ggcgatcacg
       61 aaatgatcgg cgtggacggt ggtccgggca tcgctccggc tcaccatcac ggtgtgaggc
      121 ccggtgaagt gggccgtgcc gcgcatatgt ttgaaccggt cgccgctgtt ctccagcagt
      181 tgcaagtggc aggcgtagag gtatttccgc tcgaacaccg cctcgttcaa caccttggag
      241 atctcgggcc acgagagccg gaagggctgg cggctgcgtt cgcggatcac ctcattggcg
      301 ctggtgacgc gctggctgag ttcccagagc gtcttgctca tgagcgcacc gttgaagatg
      361 ccggtgccac cgatgcgctc gcgttccacg agcacggtgc gcaggccgag gtcgatcgcg
      421 cgcatggccg cggcatagcc cgcaggtccg ccaccgatca cgcaaaggtc gaagtgttcc
      481 atggtgtgaa gtggcgcgtt catacgtcct ccggtgccaa tggttcatga gcgtggtccg
      541 gatccggcag ggtaacccgg ccggtatcgg aaacgtagca gggtggctgc cccggatccc
      601 catgtgccac ggtccgaacc gtcaccggga cttggcggcc acgaaacgat cctccaacct
      661 ccgatgacca ggcaacgctc ctccatcctg atctgcacac tcgtcgccac catttcggtg
      721 agcgcacaga ccgtccgctc caacgcatat gcacggatgc tcgatgatgc cggtggcctg
      781 ttgacggccg aacggtggga ggatgcggcg aaagtatacc gtcgtttgga gatcatggac
      841 agcacctccg ccgaagtggc gtacggtctc ggtgtctgcc tgggtcacat cccggggcag
      901 cgaacggccg ctaccgagcg tttcgcccgg gcatcccgct atggccatct ggaggcgaag
      961 taccagctgg ccttggtgct ccaccggcag cagcgtttcg atgaggccct ggccctgctg
     1021 gcctactaca agtcggccgc ggagcacgag gttcccgatg cggaggtgga tcgccaggtg
     1081 cgcatggtgg ccacggcgcg cgagctgcag gccgatcctg tgcaggtgaa catcgccaac
     1141 atgggcccgc gcatcaacag caaggacaac gattacagtc cggtcaccac ggccgatggt
     1201 gccacattgt acttcaccag ccggcgcaag gggagtacgg gcgaggcacg cgaccccagc
     1261 gggcagtacc tggaggacat ctacatggcc cggaaggagg gtggcgagtg gggcagcgcc
     1321 atgaacgtgg gtgttccgct gaacaccttc gtgcatgatg ccacggtggg tctgagtccc
     1381 gatggcggct ccatgatcat ctatcggacc gcgcagaatc tggtgagcgg ggacctgtac
     1441 gagtgcgtga aacatctcgg cgcgtgggag cccccgacga aaatgaccga gcgcatcaac
     1501 agcagcagcc atgaacccag cgccacgata gcgcccgatg gttcggagat ctacttcacc
     1561 agcgatcgcc cgggcggcat gggcggccgc gatatctacc ggatccgcaa gctgcccggc
     1621 ggggaatgga gcctgccgct caacctcggg ccggtcatca acacgccgtt cgacgaggac
     1681 gcgcccttca tgcacagcga tggcaagacg ctcttcttca gcagcaacgg gcacgccacc
     1741 atgggcgggt atgatatatt caagagcacg ttgacggacc cggacatgaa cggatggacc
     1801 gctccggaga acatgggcca tccgctcaac acggtgaacg acgacatttt cttctgcctc
     1861 agcgaggacg ggcgaacagg gtatttcagc agcgaacggg ccgatggcct cggtggtcag
     1921 gacatctaca gcatcgtgtt ccccgagagc cagctggagg agatcgtggt gcgcggtgtg
     1981 gtcaccgacg ggatggagga gcccgtgcaa gcccgcatcg tgctcacggg tgaggcggac
     2041 ggggagatgg tcggcgtgta caacaccaac gcgcgcacgg gacgttacct gatggtggtg
     2101 aaaccaggag aacggtataa tatgaccgtg gaggcccccg gcttcgttcc gcggtccgat
     2161 gcgctgagca cggtggggaa aagcggcggt cttaccgaga tcccgctcga catcatcctc
     2221 gtgcgggatg agaagacggc ccgggtgaag ccctgatcgt caggcgctgc gaccgtatcc
     2281 tgcccgttcc cgcacgcgcg ccaaggtgcg gtcggccact tcgcgggcct tctccgcacc
     2341 gatgcgcaac aaccggtcga gttcgcccct gtcgttcatc agatcgtgga agagggtgcg
     2401 ctccttggcg aacgcggtga tgagcacatc cagcagttcc ttcttggcat gaccgtatcc
     2461 gtaaccgccg cgtaggtagt tttcgcgcat cgccgtggtg cgttcgggcg ttgccgtcag
     2521 tgcgaacagc ttgaacgtgt tgtcgttgcc cggatccttg ggctcctcca gcgtgcggct
     2581 gtcgctcacg atgcccatca cctgcttctt caattccttc tccgggagga agacgttgat
     2641 ggtgttgccg tagctcttgc tcatcttcct gccatcggtg ccggggatgg tcatgagctg
     2701 ctcatcgatg cgcgccttgg gcaccacgaa ggtgtcctcc ccgtaagccc ggttgaaggc
     2761 gctggcgatg tcgcgcgtga tctccaagtg ctgcttctgg tccttgccca ccggcaccac
     2821 ctcgctgtcg taaagcagga tgtcggcggc catcagcacg ggatagttga acaccccggc
     2881 gttgggcact gaccccttgg ccagggcatc cttgtaggag tgggcattct ccagcatcgg
     2941 gcagggcgtc aggcattcca ggtaccaggt cagctcggtc acctcgggca cgtcgctctg
     3001 gcggtagaag gtggtccgct ccaggtccag ccccatggcg atccaggccg cggccacggc
     3061 gtacgtgttc tcgcgtcgca gttgcgggtc gcgcagttgg gtgaaggagt gcaggtccgc
     3121 gatgaagagg aatgcgtcat tgcccggcag gcggcccatg gcgatggccg ggacgatggc
     3181 gcccatcagg ttgcccaggt gcggtgtgcc ggtgctctgg atgccggtga ggatgcgggg
     3241 catgtgcgtg ggaaggggcc gaagatagga gggggcgtcg cgcggctttg agcgggcatc
     3301 ggggacctac tttcgcgccc ttcatcaccc gccagggcac aagcgaagac gggtccgcat
     3361 gtccatgtac caacaccacg atcagctcgt tgtttgcgac ggggagtcga tgcgggcacc
     3421 ggtactgaag tggttgttcg tggtgccgcg tgtgctgttc aaactgtgga tgttcctcgt
     3481 tttcttcaca tcgttggcgg tgttgtacgt gccgttccgc cttcggctca ggaaggaaga
     3541 ggagcatccg cgtgcgttcc gtttgatgaa cgcctggaac cgcttcctca acgtgttcct
     3601 tctggtgccg gtgaagcgcg tgtccatggc gccgctgcca ccaccgccgt acgtgatctg
     3661 cgccaaccac ggcagctacc tggacatcct gcacatgtac agcatgggac tggatttttt
     3721 cctgttcatg ggtaagtatg aattgctgaa atggccgctg ctgggcatgt tctttcgccg
     3781 gatgaacatc gccgtgaacc ggggtaatcg cacggaggcg gtgaaggccc tggcccatgc
     3841 ggcgcggtcg atcgacaagg ggatctgcgt ggcgctgttt cccgaaggca ccataccggt
     3901 ctcggcgccg cgcatgcagc gcttcaagga cggcgccttc aagctcgcca tcgagaagca
     3961 ggtgcccatc gtgccgatca ccttcctgga ccactggaaa ctgttcggcg agccgggtga
     4021 gttgtgcagc cgcggtcacc ccggcttcgc gcacgtggta cggcatccgt tcatcagcac
     4081 cgcgggcttg accatggccg acaaggaggc gctgcgccgc cgggcaggga cggtgttccg
     4141 ggtgctggag tcgggca
//
LOCUS       DAOQOC010000107         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9667_length_4142_cov_3.042085, whole genome shotgun sequence.
ACCESSION   DAOQOC010000107 DAOQOC010000000
VERSION     DAOQOC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9667_length_4142_cov_3.042085"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..358
                     /gene="tilS"
                     /locus_tag="PL106_02705"
     CDS             50..358
                     /gene="tilS"
                     /locus_tag="PL106_02705"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNA32007.1"
                     /translation="MNEVWLDADRLSFPLELRPWHTGDRIRPMGLGGSKLVSDVLIDA
                     RVPLINKADTHVLVSAGEVVWVAGHRLAEGYRAGPATRNVLHIRIEQGITFPTPSHTP
                     "
     gene            355..645
                     /locus_tag="PL106_02710"
     CDS             355..645
                     /locus_tag="PL106_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32008.1"
                     /translation="MIPSSIHRIEKEDVPALHFPVEPVHLSDEHLRLRDANIGRAMQL
                     GNLEHTKCRIVFRDSVGMKVVETTVWAFDAERIVLKKGISIPIDRVIEVDLL"
     gene            768..1220
                     /locus_tag="PL106_02715"
     CDS             768..1220
                     /locus_tag="PL106_02715"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNA32009.1"
                     /translation="MHRSILSMLFTVAVASLYGGSPIHWSFATKEGVNGQNYLALRAV
                     CDEGWHFYALTLPREDGPLPTMITVAPSPQFTSGTIIEPHAIEVEDPNFQMLVRYHGH
                     DAEFLVPIERLGKDAFNVTGNVMYMCCNDKTCLPPVTVEFSVPFPAVK"
     gene            1223..3286
                     /locus_tag="PL106_02720"
     CDS             1223..3286
                     /locus_tag="PL106_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNA32010.1"
                     /translation="MRKFRPLLPFLFPALIAILSGCGKEQPTAIADPTAIKVDPVNDK
                     VAWSVKTVPTGDNAFDLVFHADIDPGWSVYSIEEFGDGPIPSRFELDTLGHVTILPGT
                     EEKSAHVVQGHDPNFDMEVKKFKESVDFIRHFTTTASDLPVTGQFEYMTCDNSMCLPP
                     ALIFWRAVPDSGTFALSTVPFDLSGMAAKSCADGTYKLKNVDLAKPVVPGGKQDHRDS
                     SLLMIFLLGFVGGLVALLTPCVFPMIPLTVSFFTKGSENRRKGLMNALTYGGFILLIY
                     VIFSIPFHLLGSVNPEIFNEISTNPWLNIFFFVIFIIFAISFLGYFEITLPSGWVNRM
                     DSNASKFGGGIGIFFMALTLALVSFSCTGPILGSLLAGALSSDGGAWQLTMGLSGFGM
                     ALALPFALFAMFPQWLNNLPRSGGWLDTVKKTLGFIELALAVKFFSNADLVMNWGLLK
                     REVFIGLWIVIGLALVLYLTGRLRLPHDVKGAAISKGRWTTAALAFAFVLYLIPGLSN
                     GEWRNLKLLSGFPPPLFYSIYHQESECPLGLDCHHDLEEGMAVAQREGKPIMLDFTGW
                     ACVNCRKMEETVWSEPAIHAILKEKVVLVSLYVDDKRELSKDDQHVYIACDGQKKFIQ
                     NIGNRWATLESETFAQVSQPLYVLLSPDGQLLTDPVGYTPDEDDYADFFERGLKGMDM
                     LKEGK"
     gene            complement(3873..>4142)
                     /locus_tag="PL106_02725"
     CDS             complement(3873..>4142)
                     /locus_tag="PL106_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32011.1"
                     /translation="RCVKRHHRPFMGSVGGSRQVSPEDPDVFRYGPHVVRYVEQHMNR
                     LLGEGHWQLPSAKHVLHFFHRLTQWMRAMIFSSGGHGMARHDCIA"
BASE COUNT          810 a         1327 c         1162 g          843 t
ORIGIN      
        1 cggatcggga tgcgcccggt agaagacgta aagtccggtg ccgaccagca tgaacgaagt
       61 ctggctcgat gcggaccggt tgagcttccc gcttgagtta cgtccctggc acaccggtga
      121 tcgcatccgc cccatggggc tggggggcag caaattggtc agcgatgtgt tgatcgacgc
      181 cagggtgccc ctgatcaaca aggccgatac ccatgtactg gtgagtgcag gcgaggtggt
      241 gtgggtggcc ggacatcggc tggccgaggg ctaccgggcc gggccagcca ccaggaacgt
      301 gctgcacatc cgcatcgaac agggcattac ttttcccacg ccatcgcaca ccccatgatc
      361 ccatccagca tccaccgcat cgaaaaagag gatgttcctg cgctgcattt ccccgtggag
      421 cccgttcatc tgagcgatga acacctcagg ttgcgcgacg ccaacatcgg ccgtgccatg
      481 caactgggca acctggagca caccaagtgc aggatcgtgt tccgcgacag cgtgggtatg
      541 aaggtggtgg aaaccaccgt ttgggccttc gacgccgaac gcatcgtgct gaagaagggc
      601 atctccatcc cgatcgaccg tgtgatcgag gtggatctgc tgtgaccacc tcccaaaaac
      661 cgtgaacacc cagccgggca ggatccctct gatggccaga tagcgcacgg tcattttgca
      721 tgccccttgc gtacaggcgg ctaccttagc cgcccactga ccccgccatg caccgttcca
      781 tcctttcgat gctcttcacc gtggctgtgg caagccttta cggcggcagt ccgatccact
      841 ggtcattcgc caccaaggag ggggtcaacg gccagaacta tctggccctg cgcgccgttt
      901 gcgatgaggg ctggcacttt tacgccctga ccctgccgcg cgaggatggc cccctcccca
      961 cgatgatcac ggtcgcacct tcgccccagt tcacgtcggg gaccatcatt gagccgcatg
     1021 cgattgaggt ggaggacccc aacttccaaa tgctcgtccg ttaccacggg cacgatgccg
     1081 aattcctggt cccgatcgaa cggctcggca aagatgcctt caacgtgacg ggcaacgtga
     1141 tgtacatgtg ctgcaacgac aagacctgcc tgcccccggt caccgtggag ttcagcgttc
     1201 cattccccgc cgtcaagtag caatgcggaa gttccgaccc cttctcccgt tccttttccc
     1261 ggccctcatc gccatccttt ccggctgcgg caaggagcag cccaccgcca tcgccgaccc
     1321 aacggcgatc aaggtggacc cggtcaacga caaggtggcc tggtccgtga agaccgttcc
     1381 gaccggtgac aacgcgttcg acctggtgtt ccacgcggac atcgatcccg gatggtcggt
     1441 atactcgatc gaggagttcg gcgatggccc cattcccagc cgcttcgaac tggatacgct
     1501 gggccacgtg accatcctgc ccggtaccga ggagaagagc gcgcacgtgg tgcagggcca
     1561 cgaccccaac ttcgacatgg aggtgaagaa gttcaaggag agcgtcgatt tcatccgcca
     1621 tttcaccacc accgcgagtg acctgccggt cacaggccag ttcgagtaca tgacctgcga
     1681 caactcgatg tgcctgccgc ccgcgctcat cttctggcgt gccgtgcccg acagcgggac
     1741 cttcgcgctg agcaccgtgc ctttcgacct cagcggcatg gccgccaagt cgtgcgccga
     1801 cggcacgtac aagctgaaga acgtggacct ggcgaaaccc gttgtccccg gcgggaagca
     1861 ggaccaccgc gacagttccc tgctgatgat cttcttgttg ggcttcgtgg gcggcctggt
     1921 ggcgctgctc accccgtgcg tcttccccat gatcccgctc accgtgagct tcttcaccaa
     1981 gggcagcgag aacaggcgca agggactgat gaacgcgctg acctacggcg gcttcatcct
     2041 cctgatctac gtgatcttca gcatcccctt ccacctgttg ggctcggtga acccggagat
     2101 cttcaacgag atcagcacca acccgtggct gaacatcttc ttcttcgtca tcttcatcat
     2161 tttcgcgatc tccttcctcg ggtatttcga gatcaccctg cccagcggtt gggtgaaccg
     2221 catggacagc aacgccagca agttcggcgg tggcatcggc atcttcttca tggcacttac
     2281 cctggcgctg gtatcgttct catgcacggg tcccatcctc ggctccttgc tggccggcgc
     2341 gctcagcagc gatggcggtg cctggcaact gaccatgggg ctcagcggct tcggtatggc
     2401 gctcgcgctg cccttcgcgc tcttcgccat gttcccgcaa tggctgaaca atctgccccg
     2461 cagcggtggc tggctcgaca ccgtgaagaa gaccctcggc ttcatcgaac tcgcgcttgc
     2521 ggtgaagttc ttcagcaacg cggacctggt catgaactgg ggcctgctca agcgcgaggt
     2581 cttcatcggc ctgtggatcg tcatcggcct cgccttggtc ctctacctca ccggtcggct
     2641 gcgactgccg cacgatgtga agggcgccgc catctccaag ggtcgctgga cgaccgccgc
     2701 actcgccttc gcattcgtat tgtacctgat cccgggcctc tccaacggcg aatggcgcaa
     2761 cctgaaactg ctcagcggct tccctccgcc actgttctac agcatatacc accaggagag
     2821 cgagtgccct ctggggctcg attgccacca tgacctggaa gagggcatgg ccgtggccca
     2881 gcgtgaagga aagcccatca tgctcgattt caccggctgg gcctgcgtga actgccgcaa
     2941 gatggaggag accgtgtgga gcgaacccgc gatccatgcg atcctgaagg aaaaggtggt
     3001 gctggtgtca ctctacgtgg acgacaagcg cgaactgtcc aaggacgatc agcacgtgta
     3061 catcgcctgc gacggccaga aaaaattcat ccagaatatc ggtaaccgct gggccacctt
     3121 ggagagtgag accttcgccc aagtgagcca acccctttac gtgctgctca gtcccgatgg
     3181 gcaactgctc accgaccccg taggctacac acccgatgag gacgactacg cggacttctt
     3241 cgagcgcggg ctgaagggga tggacatgtt gaaggagggg aagtaggctc gcagatatgt
     3301 tggtccacag agcgctgtcg tcctgtatgc ctctgaccta tgtatggcca tacatatgtt
     3361 tcaagcctga actgctgcct gcagggctct tcctcatcca ctgacatccg ttcgctcaat
     3421 cgccggtagt tgcgccaata caggtgtacg gcacgagatc cttgacgact taggcgcttg
     3481 caggatatat gtatggccat gcatacgttt ccctgctata gaagctgctc gcggggcgct
     3541 tccacatcca ctgcacgttc gcccttcccc gatcgccgag ccaattctgg agtactgcac
     3601 gagatcctta acgaccctgg cgctcgcagg atatatgtat ggccatacat acgtttcaac
     3661 tttgagatgt tggttgcagg gctcccccga aatccacttc gacacgttcg ccctttccct
     3721 gatcgccccg ccattactga tgttctgcac gagatcctgg acgacccggg cgctcacatg
     3781 atatatgtat ggccatacat acgttccggt cgagtttgct taagcacttt tctatccgca
     3841 tcttgcccgc tcgtcacttg ccgggatcct cgtcaagcga tgcagtcatg acgagccatc
     3901 ccatgcccgc cggatgaaaa gatcatcgca cgcatccact gggtcaaccg gtgaaaaaag
     3961 tgaagtacgt gctttgccga aggaagctgc caatggcctt caccgagcag gcggttcatg
     4021 tgttgttcaa cgtaccgaac aacatgcggt ccgtagcgaa agacatccgg atcctccggt
     4081 gaaacctgcc gggagccgcc aacgcttccc ataaaaggac gatggtgccg tttgacgcat
     4141 ct
//
LOCUS       DAOQOC010000108         4094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9871_length_4094_cov_2.991582, whole genome shotgun sequence.
ACCESSION   DAOQOC010000108 DAOQOC010000000
VERSION     DAOQOC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9871_length_4094_cov_2.991582"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PL106_02730"
     CDS             complement(<1..540)
                     /locus_tag="PL106_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32012.1"
                     /translation="MRTRNGNKDARVRGCEGIPPHDRTPVRMGVLAYLLFLTILSTAQ
                     SAGKLRFSIDPPAGYQFVLDHKFRMAQQEVELSAGPHHFSFWAPQRMVTDTTLIVMAN
                     SIREVRLRLPFSNDFRLHEQALRADKRKVWTGVVLPAVLTAGAVGATVGTFIGYKRAH
                     DRLVDDRAAYTNGSDPGALA"
     gene            complement(555..2465)
                     /locus_tag="PL106_02735"
     CDS             complement(555..2465)
                     /locus_tag="PL106_02735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNA32013.1"
                     /translation="MSTTNYSEEHIRSLDWKEHIRLRPGMYIGKLGDGSSYDDGIYIL
                     LKEVLDNCIDEFVMGHGKRVEVTIEGPRVTIRDFGRGVPLGKVIDVVSKINTGAKYDS
                     KAFKKSVGLNGVGTKAVNALSTYFRIESVREGEMKVAEFDRGVLRKDNKLVRSEEPNG
                     TRDVFEPDPQLFHNFQYREQHIERLLWNYCYLNTGLTIHYNGQKFQSKNGLLDLLTTK
                     MDGEPLYPIIHLVGDDIEVAMTHAEHYGEEYYSFVNGQHTTQGGTHQGAFREAVAKTI
                     KDFFKKDWEAGDVRTGIVAAISVKVIEPVFESQTKTKLGSLEVEPGGQSMRSFVGDFL
                     ATKLDNFLHKNAEVVAALQEKILASERERKELSGIRKLARERAKKASLHNKKLRDCRV
                     HLTDPKKDERKDLTTLFITEGDSASGSITKSRNVETQAVFSLKGKPLNCFGLTKKVVY
                     ENEEFNLVQAALDIEDGMDGLRYNRIVIATDADVDGMHIRLLMITFFLQFFPDLIKND
                     HLYILQTPLFRVRDKKETIYCYSEGERVAALSKLGKNAEITRFKGLGEISPDEFKHFI
                     GPDMRLEPVMITKDASVHELLAFYMGKNTPDRQNFIIENLKVEKDMVKEETPIAAIAR
                     KLKENEMEEVEA"
     gene            3176..3538
                     /locus_tag="PL106_02740"
     CDS             3176..3538
                     /locus_tag="PL106_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32014.1"
                     /translation="MAGSDQVFIVNKDQMRAISELVANEFVVRCRGFLRQNFEELSTR
                     SDEYLDDLTRSTIQFGKELNFRKERSVQRLLAMRQQFGFTSGTDIPIKELDAITTETD
                     ETARLDHLELLLTDGQIK"
BASE COUNT          819 a         1188 c         1211 g          876 t
ORIGIN      
        1 cgccaaggcc ccggggtcgc tcccgttggt gtatgccgcc cgatcgtcca ccaggcggtc
       61 gtgggcgcgt ttgtaaccga tgaaggtgcc cacggtggcg cccacggcac cggcggtgag
      121 caccgctggc agtaccacac cggtccacac cttccgtttg tcggcgcgca gggcctgctc
      181 gtgcagccgg aagtcgttgc tgaaaggcag acgcagccgc acctcgcgga tgctgttcgc
      241 catcacgatc agcgtggtgt ccgtgaccat gcgttgcggt gcccagaagc tgaagtggtg
      301 cggcccggcg gacagttcca cctcctgttg cgccatacga aacttgtgat ccaggacgaa
      361 ttgatacccc gctggcggat cgatgctgaa gcggagcttc ccggcggact gggcagtgga
      421 gaggatcgtc aggaacagaa ggtacgcgag gacgcccatg cgcacaggtg tgcggtcgtg
      481 cggggggatg ccctcgcacc ctcgcaccct cgcatccttg ttcccgttcc gtgtcctcat
      541 ggcttccctc gggcctaggc ctccacctcc tccatttcgt tctccttcag cttgcgggcg
      601 atggcggcga tgggggtctc ctccttcacc atgtccttct ccaccttgag attctcgatg
      661 atgaagttct ggcgatcggg cgtgttcttg cccatgtaga acgcgagaag ctcatgtaca
      721 ctggcgtcct tggtgatcat caccggctcc aaccgcatgt caggcccgat gaagtgcttg
      781 aactcgtcgg ggctgatctc gccgagccct ttgaagcgcg tgatctcggc gttcttgccc
      841 agtttgctca gggcggccac ccgttcgccc tcgctgtagc agtagatggt ctccttcttg
      901 tcgcgcacgc ggaagagcgg tgtttgcagg atgtacagat ggtcgttctt gatgaggtcg
      961 gggaagaact gcaggaagaa cgtgatcatc agcaggcgga tgtgcatgcc gtccacatcg
     1021 gcatcggtcg cgatcacaat gcggttgtag cgcaggccgt ccatgccgtc ctcgatatcg
     1081 agcgcggcct gcacaaggtt gaactcctcg ttctcgtata ccaccttctt ggtaagaccg
     1141 aagcaattga gcggcttgcc cttgaggctg aacacggctt gggtctccac attacggctc
     1201 ttggtgatgc tgccgctcgc gctgtccccc tcggtgatga acagcgtcgt gaggtccttg
     1261 cgctcgtcct tcttggggtc ggtgaggtgt acgcggcagt cgcgcaactt cttgttgtgc
     1321 aggctggcct tcttggcgcg ttcgcgggcc agcttgcgga tgccgctgag ttccttgcgc
     1381 tcgcgctcgc tggcgaggat cttctcctgc agcgcggcca ccacttcggc attcttgtgc
     1441 aggaagttgt ccagcttggt ggcgaggaag tcgcccacga aggagcgcat gctctggcca
     1501 ccgggttcca cctccagcga accgagcttg gtcttggtct ggctctcgaa cacgggctcg
     1561 atcaccttca cgctgatggc cgccacgata cctgtacgca catcaccggc ttcccagtcc
     1621 ttcttgaaaa agtccttgat cgttttggcc acggcctcgc gaaaggcccc ctgatgggtg
     1681 ccaccctgcg tggtgtgctg gccgttcacg aagctgtagt actcctcgcc gtaatgttcg
     1741 gcgtgggtca tggccacctc gatatcgtcg cccaccaggt ggatgatcgg gtagagcggt
     1801 tcgccgtcca tcttggtggt gagcaggtcc agcaggccgt tcttgctctg gaacttctgc
     1861 ccgttgtagt ggatggtgag gcccgtgttg aggtagcagt agttccacag caggcgctcg
     1921 atgtgctgct cgcggtactg gaagttgtgg aagagctgcg ggtccggctc gaagacgtcg
     1981 cgcgtgccgt tgggctcctc ggagcgcaca agcttgttgt ccttccgcag cacaccgcgg
     2041 tcgaactcgg ccaccttcat ctcgccctcg cgcacgctct cgatgcggaa gtaggtgctg
     2101 agggcgttca ccgctttcgt ccccacgccg ttcaggccca cgctcttctt gaaggccttg
     2161 ctgtcgtact tggcgccggt gttgatcttg ctcaccacgt cgatcacctt gcccaagggc
     2221 acgccacgac cgaaatcacg gatggtgacg cgcggccctt cgatggtgac ctccacccgc
     2281 ttgccatggc ccatcacgaa ttcgtcgatg caattgtcca gcacctcctt cagcaggatg
     2341 tagatgccgt cgtcgtaact gctgccatcg cccagtttgc cgatgtacat gcccgggcgc
     2401 aggcggatgt gctccttcca atcgagggag cggatgtgtt cttcagaata gttggtggtg
     2461 ctcatgcttg gtccgccgaa gttggatgcg aaggcggtgc ggtgcggtgg cctgcccggg
     2521 tgcgtgcggt ggagtgcaag ggtctgaggg tgagaaggtg cgagggtgag cggtggaagg
     2581 cggcccttcc tttaccctcg caccaagaac aggtcctctt ggcactcctc ccgaacgcta
     2641 caaccccgat ctggcgcggg ttctgtctat agggagcgaa attagccgcg tggggcgcgg
     2701 gatccggaca gaacgaggaa cggggttatg aacgatcggg gggggagttt tcaacaagaa
     2761 gggggagaca tctacatcca aaggttgcat aaccaaccgt cgagcacgag tcgcccaaag
     2821 agcgaattgc acccgttgtg gtacactcca cattgaaatc ggtcatcact tgagaggtac
     2881 aagtgacgcc cacagaaatt caccgtgtag aagcaacatg gaaccgggtt ccatccctga
     2941 cgtccatccg gtcgcgtcca aaccgctggt ttcaatgggc gacatggaac cagacttggc
     3001 ctgagaatca gcattcatgc atgaacatgc acgatccaca cgaactatcg ggtatccccc
     3061 ccggccaaca gcctcatatt gtacaagagg acgagcagat gaggacgtcg tacaaaaaat
     3121 tccgatagct ttggttcaaa gacatcaggt ccgacggaaa aggatcattc aacccatggc
     3181 aggatcggat caggtcttta tcgtgaacaa ggatcagatg cgagcgatca gcgaactggt
     3241 tgcgaatgaa ttcgtggtac gatgtcgagg cttccttcgc cagaatttcg aagaactatc
     3301 cacccgaagt gatgaatacc tggacgatct caccagatca accatccaat tcggtaaaga
     3361 gctgaacttc cgcaaggagc gtagcgtgca gcgtctgctc gccatgcggc agcaattcgg
     3421 tttcacctcg gggacggata tcccaataaa ggagcttgac gcgataacta cggagacaga
     3481 cgaaacagct cgtttggacc acttggagct tctgctcact gacggccaga tcaaataatg
     3541 gatcagatct gagcacagat gccacgagga acgatcaact attccgccgc tgacacggaa
     3601 gtaggtgcaa caagtattga gtgtggcact ggaacagata ccccaccggg ggtaacctgc
     3661 gagcatggcg tgatccagtt gggcccgaca actggtgaag gcaaggatca ctggatcgaa
     3721 gccgctcccg gagccgcata cggatttgag atcatctccg gtggtgtgaa caaaccgaat
     3781 cctcacctgc tacgtgtaag cggcatagca cagtcgaatt gcgaacgcca tcaggatagg
     3841 ctgatagcag cattcgttca tggtgcggac ccgatcatca attcatatgc ggagaatacc
     3901 attccgatca aatacccgta ctcggaacgg atcaccgagc tctggcaccc atcgccaagc
     3961 aagggatccc tatgcatcaa cacatgcaag ttccgatctg aaaagatcac gatcctcatt
     4021 ttccccgacc ggaagtggtc attcaagatc acattgaccg gatccgcgtc caacaaggat
     4081 ggattgaaat tcag
//
LOCUS       DAOQOC010000109         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9877_length_4093_cov_3.593115, whole genome shotgun sequence.
ACCESSION   DAOQOC010000109 DAOQOC010000000
VERSION     DAOQOC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9877_length_4093_cov_3.593115"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..583)
                     /locus_tag="PL106_02745"
     CDS             complement(146..583)
                     /locus_tag="PL106_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNA32015.1"
                     /translation="MNWGKGLALAMVAFAGMMAWFAIKASRHPSPLVTEDYYSAELRF
                     QGRIDEAARAKALSAPIQFNLTRGSVHLDLPAEMQGRLVTGTLTLLRPNDPRADRTIQ
                     LPGTSTGQVDVVGMDLLPGRYNASLEWQAGNVTYFTEEKVYVP"
     gene            complement(693..2150)
                     /gene="ccoG"
                     /locus_tag="PL106_02750"
     CDS             complement(693..2150)
                     /gene="ccoG"
                     /locus_tag="PL106_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNA32016.1"
                     /translation="MAPQPAHIKRNASARSRSSPDESYRDAISTVDKAGKRVWVYPKK
                     PGGKFTRWRQWVGYSLVVLLFAGPFMRIGGEPLLLINLVERRFVFFGQTFWPQDFLIF
                     VLGFLAFIVFVALFTVAFGRLFCGWVCPQTVFMEHVFRRIEYAIEGDWKQQQALNKAP
                     LSSDKVIKKTTKHLVFFAISFLIGNTFLAYIIGSDELLRIIREPAAQHLGGLTTMMVF
                     SGVFYGNFAFFREQACTTVCPYGRLQGVLLDRKSIVIAYDHVRGEARGLFRKGEVRND
                     AGKGDCIDCKACVHVCPTGIDIRNGTQLECVNCTACMDACDHMMTSVGLPTGLIRYAS
                     EAGIADKKPFTFNTRLKAYSGVLVAIIGVLVTLIAMRSDVDSTLLRTPGMLYQERPDG
                     RISNLYAIKLVNKTQREMPVRLDLLDLEGEVQMVGKPIDLKPGELAQGELFIILRKEQ
                     LDGMRTNVVVGVYSGDKLVDKVKTSFVGPTGGPRP"
     gene            complement(2151..3308)
                     /locus_tag="PL106_02755"
     CDS             complement(2151..3308)
                     /locus_tag="PL106_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNA32017.1"
                     /translation="MHNRSMFQRTLTTTLLSASALLSVAQDVAQAPEATSFHVTNNLL
                     YALLGLAVVQVIFIISLAGILRTMGGSGDWVRRVVQKGGRGAAMVTLCLIAGEVSAQA
                     YKGDGTSVSLNTAFWWLFITNVLLFLILLVQMNVLRELTRAVVGTTEVVREQRASGPS
                     WTNRLMKKLTRQASIEEEKELELRHDYDGIRELDNVLPPWWLWLFYGTIIWGFVYLVN
                     VHVLNIWPDTQDAYKAEMAQAKADVDAYLATQAHVVDENTVTFTDDAAVTGQGHALFT
                     QYCTPCHGADASGSETSVGPNLTDAYWLHGGGVKNIFKTIKYGVPAKGMIAWKAQLQP
                     AEIRSITCYIMTLEGKGGPTQKAPQGEEWHDSGASADTTALATDTVRTAMR"
     gene            complement(3295..3501)
                     /locus_tag="PL106_02760"
     CDS             complement(3295..3501)
                     /locus_tag="PL106_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HNA32018.1"
                     /translation="MLKFIKHHMDTIAGIGIYPVVSFLLFFLIFLGVLLYVRRSRREH
                     ITYMAALPLGDGEDANGSNNHAQP"
     gene            complement(3505..>4093)
                     /gene="ccoO"
                     /locus_tag="PL106_02765"
     CDS             complement(3505..>4093)
                     /gene="ccoO"
                     /locus_tag="PL106_02765"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNA32019.1"
                     /translation="SNVPTISSVKPYTPLELQGRDIYVREGCYTCHSQMIRPFRGETE
                     RYGEYSKAGEYVYDHPFQWGSKRTGPDLHRVGGKYPDSWHYYHMWDPTTMSPGSLMPA
                     YPALFQDALDTTTTRRKIEAMITLGVPYEKEYGADFPLRANAHLVEQSRKIAANLKGA
                     GVETPIDMEIVALIAYLQRMGTDIKAAPASTTAAK"
BASE COUNT          723 a         1279 c         1264 g          827 t
ORIGIN      
        1 gttcaggagc agcgtgcttc gcagccgcgc tcccgtgccg gtcccggaag atggtaccgc
       61 cagtgctatg ggcccgcaca tgccgatgca gtgggcgctt ccggcgaccc ccagcacgag
      121 cgcggtggcg aacatcgggt ccatgctacg gcacgtatac cttttcttcg gtgaaatagg
      181 tcacgttccc ggcctgccat tccagcgatg cgttgtaccg gcccggcaac aggtccatgc
      241 cgaccacgtc gacctggccg gtggatgtcc ccggaagctg gatcgtacgg tcggcacgcg
      301 gatcgttcgg ccggagcagc gtcagggtcc cggtcaccag gcgtccttgc atctcagcgg
      361 gcagatccag atgcaccgac ccccgggtca ggttgaattg gatgggcgcg ctcaaggcct
      421 tcgcccgggc ggcctcatcg atccggccct ggaatcgcag ttccgcactg tagtaatcct
      481 cggtcaccaa tggtgatgga tgtcgggagg ccttgatcgc gaaccaggcc atcatgcccg
      541 cgaaggcgac catcgccagt gccagccctt ttccccagtt catgggtatc tattgttcct
      601 gttgggcgtc cccggttgat cctcacggac ccgcacgggg tcatgcggaa cgacacggtc
      661 ggtgcgggtc gccgatagtt gaacggtttc cctcatggcc gtggccctcc tgtcggccca
      721 acgaacgagg tcttcacctt gtcaaccagc ttgtccccgc tgtacacacc caccaccacg
      781 ttcgttctca tcccatcgag ctgctctttg cgcaggatga tgaacaactc gccctgggcc
      841 agttcaccgg gcttcaggtc gatcggtttc cccaccatct gcacttcgcc ttccaggtcc
      901 aggagatcca gccgtaccgg catttcgcgt tgggtcttgt tcaccagttt gatcgcgtaa
      961 agattgctga tccgcccatc gggtcgttcc tggtagagca tgcctggtgt ccgcaggagc
     1021 gtgctgtcca catcgctgcg catggcgatc aaggtcacca gcacgccgat gatcgcgacg
     1081 agcaccccgc tgtacgcttt caaccgggtg ttgaaggtga agggtttctt gtcggcgatc
     1141 ccggcttcgc tcgcgtagcg gatcagcccc gtgggcaggc ctacgctcgt catcatatgg
     1201 tcgcacgcat ccatgcaggc cgtgcagttc acgcactcga gctgggtgcc gttccggata
     1261 tcgatcccgg tggggcacac gtgcacgcac gccttgcaat cgatgcagtc ccccttgccc
     1321 gcgtcgttcc gcacctcgcc cttgcggaag agcccgcgtg cttcgccgcg aacatgatcg
     1381 tacgcgatga cgatgctctt gcgatcgaga agcacgcctt gcaggcgccc gtaggggcac
     1441 accgtggtgc atgcctgctc ccggaagaag gcgaagttgc cgtagaacac gccactgaat
     1501 accatcatgg tggtaagccc gcccagatgc tgtgcggccg gttcgcggat gatccggaga
     1561 agctcgtcgc taccgatgat gtatgccagg aaggtgttcc cgatcaggaa gctgatcgcg
     1621 aaaaagacca ggtgcttcgt ggtcttcttg atgaccttgt cactcgacaa tggtgccttg
     1681 ttgagtgcct gctgctgttt ccagtcgccc tcgatggcgt attcgatccg gcggaacacg
     1741 tgctccatga acaccgtctg cggacatacc cagccgcaga acaaacgccc gaaggccacc
     1801 gtgaacaacg ccacgaacac gatgaaggcc aggaaaccca gcacgaagat caggaagtcc
     1861 tgcggccaga acgtctgccc gaagaacacg aagcgccgtt ccaccaggtt gatcagcagc
     1921 aagggctcgc caccgatgcg catgaacgga cctgcgaaca acaggaccac cagggaatat
     1981 cccacccact gccgccatcg tgtgaacttg ccgccgggtt ttttcgggta cacccaaacc
     2041 cgcttgccgg ccttgtccac ggtgctgatc gcatcgcggt agctttcatc cggcgaggac
     2101 ctcgacctgg cggaggcgtt ccgcttgata tgggccggct ggggagccat ttaccgcatg
     2161 gccgtgcgta cggtatccgt ggcgagggcc gttgtgtcgg cagatgcacc gctatcgtgc
     2221 cattcctcgc cctgcggggc tttctgtgtc gggccgccct tgccttcgag cgtcatgatg
     2281 tagcaggtga tcgagcggat ctcggccggc tgcaattgcg ccttccaggc gatcatgcct
     2341 ttcgcgggca cgccgtactt gatcgtcttg aagatgttct tcaccccgcc gccgtgcaac
     2401 cagtacgcat cggtgaggtt gggccccacg ctggtctcgg agccggaggc gtccgcgcca
     2461 tggcagggcg tgcagtactg cgtgaagagg gcgtggccct gaccggttac cgccgcatcg
     2521 tcggtgaagg tcacggtatt ctcgtccacc acatgggcct gcgtggccag gtaggcgtcc
     2581 acgtccgctt tggcctgtgc catctcggcc ttgtacgcgt cctgcgtatc gggccagatg
     2641 ttgagcacat gcacgttcac caggtatacg aaaccccaga tgatggtgcc atagaacagc
     2701 cacagccacc agggcggcag gacgttgtcc agttcccgga tgccgtcgta atcatggcgc
     2761 agctcgagct ctttttcctc ctcgatggag gcttggcgcg tgagcttctt catgaggcgg
     2821 ttggtccaag agggccctga tgcgcgctgt tcgcgtacga cctcggtggt gcccaccacg
     2881 gcacgcgtca gttcgcgaag gacattcatc tgtacgagca ggatgaggaa caggaggacg
     2941 ttggtgatga agagccacca gaaggccgtg ttcagcgaca cgctggtacc atcgcccttg
     3001 tacgcctgcg cgctgacctc tccagcgatc aggcaaaggg tcaccatggc ggccccgcgc
     3061 ccaccttttt gtaccacgcg ccgcacccag tcgcccgaac cgcccatggt gcgcaggatc
     3121 ccggccaggg agatgatgaa gatgacctgc accacggcca gtccgagcag ggcgtacagc
     3181 aggttgttcg ttacatggaa ggacgtggcc tccggtgcct gtgcgacatc ctgcgcaacg
     3241 gagagcaggg ctgatgctga caggagggtg gtggtcagag tgcgctggaa catgctacgg
     3301 ttgtgcatgg ttgttggatc cgttcgcgtc ctccccatcg ccgagcggaa gggccgccat
     3361 gtatgtgatg tgctcacggc gcgaccgccg gacatagagc agtacgccga ggaagatgag
     3421 gaagaacagc aggaacgaca ccacgggata gatcccgatc cccgcgatcg tatccatgtg
     3481 gtgtttgatg aatttcagca tggctcactt cgccgcggtg gtgctggcgg gcgcggcctt
     3541 gatatcggtg cccatgcgtt gcaagtaggc gatcaacgcc acgatctcca tgtcgatcgg
     3601 ggtctccaca ccggccccct tcaggttggc cgcgatcttg cggctctgtt ccaccaggtg
     3661 cgcattggca cgaaggggaa agtcggcgcc gtattccttc tcgtacggca cccccagggt
     3721 gatcatcgct tcgatcttcc tgcgcgtggt ggtggtgtcc aaggcgtcct ggaaaagcgc
     3781 gggatacgcc ggcatgagcg atccggggct catggtggtg gggtcccaca tgtggtagta
     3841 atgccagctg tccgggtatt tgccccccac acggtggagg tcggggccgg tgcgtttgct
     3901 gccccactgg aagggatggt cgtacacgta ttcgcccgcc ttgctgtact cgccgtaacg
     3961 ttccgtctca ccgcggaagg ggcggatcat ctggctgtgg caagtgtagc agccctcacg
     4021 cacataaata tccctgcctt gcagttccaa tggcgtgtac ggcttcacgc tgctgatggt
     4081 gggcacgttg ctc
//
LOCUS       DAOQOC010000110         4082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_9922_length_4082_cov_3.897194, whole genome shotgun sequence.
ACCESSION   DAOQOC010000110 DAOQOC010000000
VERSION     DAOQOC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9922_length_4082_cov_3.897194"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PL106_02770"
     CDS             complement(<1..942)
                     /locus_tag="PL106_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNA32020.1"
                     /translation="MRRRTIGTIVLLALLSMAGVVTTQYIWLRRAQRYQSEQVQLQKQ
                     QAAQLDKQFNDRVVIALTDVTQRILSINKDPSDLFDAVKQVSPNYYAVSINDTIHPYL
                     LEELLKHEFERRGISEDFEYGIYDCFTDSLVYGDYVGAADTMRQAGVLPKLNKDGHYF
                     AVNFPRRLSSLWEPEDSGTITWIYPAIVTLIVFAFFMYSVWTILRQKRLGEMKNDFIG
                     NMTHEFKTPISTIALSSEVIGDPAIIQDPDRLRCYAEIIRSENERLRTQVERVLQLST
                     LDRGELHLKHEQVDMHRVIRDVAASYRLQLAEREGEVE"
     gene            complement(1089..3593)
                     /locus_tag="PL106_02775"
     CDS             complement(1089..3593)
                     /locus_tag="PL106_02775"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA32021.1"
                     /translation="MRYTFLLLLMLVLASPAEVRAQRHTMGGERPRVRVFGRVVDGAT
                     RKGVEFATINVLAAGKDSIVGGALADEHGDFSMGDMPVGAYRLRIASLGYSNLEQQIQ
                     VRPGSDEQDLGNLTLAPAATELKAAEVTAERAQTQLQVDRRVYNVDKDLSVRGGNGLD
                     VMKNVPGLSVDADDNVQLRNGTPQILLDGRPTQLTLDQIPADDIERVEVITNPGVAFD
                     ASSGAGIINVVLKRSTRPGYNGQVQLNAGTNRRYGGNASINVKDGRWSWNANLNGGTS
                     ENRTNSPTSRDTYAEGAVVQSFHQDGTAWNRRSRYGGRAGGEVQLTNRSTLGLNVNLR
                     GRAMENKDDLIWTESDGAGTLLLGGGQLNSGNNSGYYLGSQATFKHRTPVEGREWTTD
                     LTWNRSHSTNGSDFGTTTNGSDGTPIPGGARTRITTGVSDADRLTWQFDMADPRSERT
                     RSNYGLRSNVNVQRSLLDVVSGSDTSIAAPDPSLSNDYRITDIVNAAYFNWTRKLGTH
                     WGMQAGVRFEQTWFEADVRDPSGTRHQKYTYNYPASWSGIGRALFPAIYLSRKWEGSS
                     REFQVNVSRKIERPNFFQVMPIIMSSDTRNVRIGNPALAPEFITLAEVNHLLPFRKDK
                     RSNWLTSVFARYTDNVITGWAYPMPTDSNVLVNSWINGRDSWTWGWENAVKLDLFKST
                     QLTLSGTMQYVEIAAGPPFNGRKSGWMGSAKANLSIRLPKDWSIQINGEYEGKRPQPQ
                     GYRIPNGGMDVSLSKDLGKHWNVQATMEDVFYTRLWGTIIETPYLYQESYRRHSMRNA
                     RVSVSWKFGEQDASLFRRRGEGPRRDPGSESTPGEL"
     gene            complement(3734..>4082)
                     /locus_tag="PL106_02780"
     CDS             complement(3734..>4082)
                     /locus_tag="PL106_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNA32022.1"
                     /translation="RELFEAFGEVTSAKIIMDKATNRSRGFGFVEMSDDTAGKAAIEG
                     TNGKPFHGRDLVVNEARPRTEGAGGGGGFRSGGDRGGYRDRGGDRGGYRDGGGYRERD
                     RGGDRDSYRRDEY"
BASE COUNT          690 a         1318 c         1204 g          870 t
ORIGIN      
        1 ctccacctca ccctcgcgct ccgccagctg caaccggtac gaagccgcca catcgcggat
       61 cacccggtgc atgtcgacct gctcatgctt cagatgcaac tcgccccggt ccagcgtgct
      121 caactggagc acccgttcca cttgcgtgcg cagacgttcg ttctccgaac ggatgatctc
      181 cgcgtagcaa cgcaaccggt cggggtcctg gatgatggcc ggatcaccga tcacctcgct
      241 gctcagggcg atcgtactga tgggggtctt gaactcgtgg gtcatgttgc cgatgaagtc
      301 gttcttcatc tcccccaagc gcttctgccg caggatcgtc cacacactgt acatgaaaaa
      361 ggcgaatacg atcagcgtaa cgatggcggg atagatccac gtgatggtgc cgctgtcctc
      421 cggctcccac aggctcgaca ggcggcgcgg gaagttcacc gcgaaataat gcccgtcctt
      481 gttaagtttt ggaaggacgc ccgcttgacg catcgtatcg gccgctccaa catagtcgcc
      541 gtacacgagg ctgtcggtga agcagtcgta aatgccgtac tcgaagtcct cgctgatgcc
      601 gcgtcgctcg aactcgtgct tgagcaactc ttccaacaga tacgggtgga tggtgtcgtt
      661 gatcgagacg gcatagtagt tcggcgatac ctgcttgacc gcatcgaaca gatcgctggg
      721 gtccttgttg atcgacagga tgcgctgggt gacgtcggtg agcgcgatga cgacccgatc
      781 attgaactgt ttgtccaact gggcggcctg ttgcttctgc aactgcacct gttcgctctg
      841 atagcgttgc gcacggcgga gccagatgta ctgggtggtc acgacccctg ccatgctcag
      901 cagggcgagc agtacgatgg tcccgatggt gcgtcggcgc atctgttcct cgtgccccat
      961 tttccggacc cttgtacagg tggcctggtc cgggtgcgag cgtggcacaa tgataggccg
     1021 atggccgcac cgggcccggc ccggttaacg atccttccat agcccgatcg ttcgccaggg
     1081 tcggatcctc agagttctcc cggggttgat tcgctccccg gatcacgacg cggcccttct
     1141 ccccgtctgc ggaacaagga tgcgtcctgc tccccgaatt tccacgaaac cgacacccgg
     1201 gcgttccgca tcgaatgccg acgatagctc tcctgataga gatacggcgt ttcgatgatg
     1261 gtgccccaaa gccgcgtgta gaacacatcc tccatcgtgg cctgcacgtt ccaatgcttt
     1321 ccgagatcct tggacagact cacgtccatg ccgccattgg ggatacggta cccttgcggc
     1381 tgcggccgtt tgccctcgta ctcgccgttg atctggatgc tccaatcctt gggcaggcga
     1441 atactgagat tggccttcgc actgcccatc cagccgctct tgcgaccatt gaacggcggc
     1501 cccgcggcga tctccacata ctgcatggtg ccgctgaggg tgagttgggt gctcttgaac
     1561 aggtccagtt tcacggcgtt ctcccagccc caggtccagc tgtccctgcc gttgatccag
     1621 gagttcacca gcacattgct gtccgtgggc atcgggtacg cccagcctgt gatcacgttg
     1681 tcggtgtacc gtgcgaacac cgatgtgagc cagttgctgc gcttgtcctt gcggaacggc
     1741 aacaggtggt tcacctcggc cagtgtgatg aactccggcg ccagtgcggg gttgccgatg
     1801 cgcacattcc gcgtgtcgct ggacatgatg atgggcatca cctggaagaa attcggacgc
     1861 tcgatcttgc gcgacacgtt gacctggaat tcgcgcgagg acccttccca cttccggctc
     1921 aggtagatcg cggggaacag ggcccgaccg ataccgctcc aactggcagg atagttatag
     1981 gtatatttct ggtgacgcgt gcccgatggg tcgcgcacat cggcctcgaa ccaggtctgt
     2041 tcgaaacgca ctccggcttg catgccccaa tgcgtgccga gcttccttgt ccaattgaaa
     2101 tacgcggcgt tcacgatgtc ggtgatgcgg taatcgttgc tcaacgacgg atcgggcgct
     2161 gcgatgctcg tatcgcttcc ggacaccaca tccagcaaac tgcgttgaac gttgacgttg
     2221 cttcgcaggc cgtagttgga tcgcgtccgt tcgctgcggg gatcggccat gtcgaactgc
     2281 catgtcaacc ggtcggcatc gcttaccccg gtggtgatcc gtgtgcgcgc cccgccgggt
     2341 atcggagtgc catcgctgcc gttggtggtg gtcccgaaat cggaaccgtt ggtggagtgg
     2401 ctgcggttcc acgtcaggtc ggtcgtccac tcccgaccct ccaccggcgt gcggtgcttg
     2461 aacgtggcct gcgagcccag atagtaaccg ctattgttac cgctgttcaa ttgtcctcct
     2521 cccagtaaca gggtaccggc cccatcgctc tcggtccaga tcaggtcatc cttgttctcc
     2581 atcgccctgc cacgcaggtt cacattcaag cccagggtgc tcctgttggt cagttgcacc
     2641 tccccacccg ctctcccgcc gtaccgcgat cggcggttcc atgcggtccc gtcctgatgg
     2701 aaggactgga caaccgcccc ctcagcgtac gtatcccgcg aagtcggcga gttggtgcgg
     2761 ttctcgctcg tccccccgtt gaggttcgcg ttccaggacc agcgcccatc cttcacgttg
     2821 atgctggcgt tgccgccgta ccgcctgttc gtgcccgcgt tcaactgcac ctgaccgttg
     2881 tagccgggcc gggtgctgcg cttgagcacc acgttgatga tgcccgctcc gctgctcgca
     2941 tcgaaagcca cacccgggtt ggtgatcacc tccacacgct cgatgtcgtc ggcggggatc
     3001 tggtccaggg tgagttgtgt gggtcggcca tccaggagga tctgcggcgt tccgtttcgc
     3061 agttgcacgt tgtcgtcggc gtccacgctg agacccggta catttttcat cacgtccagg
     3121 ccgttgccgc cacgcacgct caggtccttg tccacgttgt acacccggcg atccacctgc
     3181 aactgtgttt gcgcgcgctc cgcggttacc tcagcggctt tcaactcggt ggccgctggt
     3241 gccagcgtca ggttgccgag gtcctgttcg tcggagcccg gccgtacctg gatctgttgc
     3301 tccagattgg aatagcccag tgatgcgatg cgcaaccggt atgcgccaac gggcatatcg
     3361 cccatgctga agtccccatg ttcatcggcc agggcaccgc ccacgatact gtccttgccc
     3421 gccgccagca cattgatggt ggcgaactca acacctttcc gggtcgcccc gtccaccacg
     3481 cggccgaaga cacgcacacg cgggcgctct ccgcccatgg tgtgccgttg cgcacgcact
     3541 tcagcagggg acgcaaggac cagcatgagc aacaggagga acgtgtaccg catgtgggcc
     3601 gcgaaattag gccggtgggc aaggggttgc cggacaaggc ggatccggtc tgcgaacaag
     3661 tcgaacggcc ttcggtgaac acaaaaggaa agggcccccg atgatcgggg gccctttcct
     3721 gtcctgttga ttcttagtac tcgtcgcgac gatagctgtc gcgatcacca ccacgatcac
     3781 gctcgcggta tccgccgccg tcccggtaac cgccacggtc accaccacgg tcgcggtagc
     3841 caccacggtc tccaccgctg cggaaaccgc cgccgccgcc agcaccttcg gtgcggggac
     3901 gtgcttcgtt caccaccagg tcgcggccgt ggaacggctt gccgttggtg ccttcgatgg
     3961 ccgccttgcc ggcggtatcg tcggacattt ccacgaatcc gaagccacgg ctccggttgg
     4021 tggccttgtc catgatgatc ttggccgagg tcacttcacc gaaagcttcg aaaagttcgc
     4081 gg
//
LOCUS       DAOQOC010000111         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10047_length_4057_cov_3.569215, whole genome shotgun sequence.
ACCESSION   DAOQOC010000111 DAOQOC010000000
VERSION     DAOQOC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10047_length_4057_cov_3.569215"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PL106_02785"
     CDS             complement(<1..294)
                     /locus_tag="PL106_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32023.1"
                     /translation="MKHLSFPLALLCSAIIFTGCQKEDEPAAPGGNTGGGNTGGGFTP
                     PTTSYWRINGIANNESMDAVSVNIAGNTLGVAKPFSDAGYGYCHDHWPVQCGPG"
     gene            459..1352
                     /locus_tag="PL106_02790"
     CDS             459..1352
                     /locus_tag="PL106_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32024.1"
                     /translation="MKRLSTLPALLVSVCVAAQPVLEYANVQLMGTSYAVHLVTDPGS
                     SDPDLDGANVTWDFSSAALDLNAGTASFVAPSTTPYAGDYPSSNLAQAVVLPGGTSYN
                     YFNLTSTQLDMLGEGIGGSDPTVYTDPKTPLQFPFSYPNWFIDYYAYDGTDYSVSRAY
                     MGYGTVILPIGTFTNVVKMASTSGSIDFFTSNPVTHLVSIDEDGVALVFGPAEVGVDE
                     TIARPVLTAFPNPSASYVRISGIELPGTWQLLDMQGRSVRSGRHGAGSLNLDLTQEAS
                     GAYTLLIQDAQGRRSVRLARM"
     gene            1470..3050
                     /locus_tag="PL106_02795"
     CDS             1470..3050
                     /locus_tag="PL106_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNA32025.1"
                     /translation="MRTILSLLLLCPLSLLAQNEFPTVWQSEFALASGWNATTPDLSY
                     VIGGDASAVEVLDGTTGKQLWGFKFKEKYGVKECEDWTADHDTETVNVELKKEKKGPV
                     TVIHLDYRTGAEVGADQLPARKKVHEAHRSKWSSRGLRVHHSECYDETSGTTISLAYD
                     DKLFKNAMGGRMTNLTVTASGGHAWSTSFEGKVVAHMIHDMLPAAEGPVILNVLTAHG
                     KVLVVYEGITCLDLATGKVLWNTTFDYTTTSVGLKAKQEIGRAAMPLVTADGVYIADL
                     SKGQRNMKKLDSNTGAVLWSADKLDGDDIVSELLIAGGNLIARFGGVVRHEMFVPNPN
                     GGVGDGTYKVEYEFEGRTGLRAYNATTGQAVWNTSDMELADNFAKSECNILSDGGQIH
                     ALGEKNIYLFDAATGKVTKQTEYNSKTIGKARSYYAYDGKFLIEGEKGIAAVDKDLAL
                     KYATNTGKCLMSEMRGDAFIVWTGKEATDRKEFIRFDPTTGTIMGKLEGCYRPRFDLT
                     GDRFVRFDDEKVIMYRTN"
     gene            complement(3061..>4057)
                     /locus_tag="PL106_02800"
     CDS             complement(3061..>4057)
                     /locus_tag="PL106_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32026.1"
                     /translation="VSAPVTARPVAVISGEYYWDTDPGAGSGIPLSALDGDFDEVLEV
                     AVQNASEELPAGPHLLGVRVLGSDGGWSAPFLQAVHVTAPVVQNLPVTLSAFLQGPLT
                     SATTMSDALRTNNLIPLTEPFTALGYNHVGSGGETINPMVLTILYGQVVDWIFVELRD
                     KDDPSIVLQTRCGLIKNNGTIVGTNGASPISFNALAGDYHLCVKHRNHLGVMTHDPLA
                     LSASGAALDLRVPSTPTYGTNARATTFGRRALWSGDATGNGIIKYTGGGNDRDPILVT
                     VGSTTPNNVVPGVYSTRDVNMNGQVKYTGGGNDRDPILLNVGSTTPNNIRPAQLP"
BASE COUNT          830 a         1215 c         1231 g          781 t
ORIGIN      
        1 cccaggcccg cactgaacag gccaatggtc gtggcaatag ccgtagccgg catcgctgaa
       61 gggtttggcc acccccaggg tgttgcccgc gatgttcacg ctcaccgcat ccatgctctc
      121 gttgttcgct atgccgttga tcctccagta gcttgtggtg ggtggggtga agccaccgcc
      181 ggtgttaccg ccaccggtgt tcccaccggg agcggcgggt tcatcctcct tctgacagcc
      241 ggtgaatatg atagcgctgc agagcaacgc gagtgggaat gacaggtgtt tcatggtgtg
      301 ccttgtatat gcgaacttgg gtcgagggtt ccaccgacgg caataccact ttgatgtgaa
      361 gagggccgtt gtgcgcattt cgtagcgagc ctcgcatgaa agtggtattg gccttccgga
      421 acatgctcgt gcagttttgc gttccgatcc tcacaaccat gaaaagactc tccaccctcc
      481 ccgctcttct ggtctccgtg tgcgttgcgg cacaacccgt actggaatac gccaatgtgc
      541 agctcatggg tacttcgtac gccgttcacc tggtcaccga tcccggctcc agcgaccccg
      601 acctcgatgg ggcgaatgtg acctgggact tcagcagtgc tgcactggat ctcaacgccg
      661 gcaccgcatc cttcgtcgcg ccatccacca caccgtacgc gggggactac ccatcgagca
      721 accttgccca ggcggtggtt cttccaggcg gtacgagcta caactacttc aacctcacga
      781 gtacgcaatt ggacatgctc ggcgaaggca tcggtggaag cgaccccacc gtgtacaccg
      841 atccgaagac gcccttgcag ttcccgttca gctacccgaa ctggttcatt gattattacg
      901 cttacgatgg gaccgattac agcgtatcac gtgcatacat ggggtatggc acggtgatcc
      961 tgccgatcgg caccttcacg aacgtggtga aaatggcgag caccagcggc tccatcgact
     1021 tcttcaccag caacccggtg acacacctgg tgagcatcga cgaggatggg gtcgcgctgg
     1081 tcttcggacc ggccgaggtc ggagtggacg aaaccatagc gcgacccgta ctgacggcgt
     1141 tcccgaatcc gagcgcatcc tatgtccgca tttccgggat cgaactgccg gggacctggc
     1201 aattattgga catgcaaggt cgcagcgtcc gttccggcag gcatggtgct ggttcgctca
     1261 accttgacct cacccaggag gccagcggag cgtacacctt gctcatccag gatgcccagg
     1321 gacggcgcag cgtgcggctt gcgcggatgt aggcttccaa ccttcacaag atcactcctc
     1381 accccatcat cgccgtcgcg aaccatcaac agcgtctgtg ccagcagacg cgatcctcac
     1441 gaacgatcac catacctcaa ccattgaaca tgcgcaccat cctttccctg ctgttgctct
     1501 gcccgctgag cttgttggcc cagaacgaat ttccgaccgt ctggcagtcc gagttcgcac
     1561 tcgcatccgg ctggaatgcc acgacacccg acctcagcta tgtgatcggg ggcgatgcaa
     1621 gcgccgttga agtgttggac ggcaccactg gaaagcagct ctggggcttc aagttcaagg
     1681 agaaatacgg cgtgaaggaa tgcgaggact ggactgctga ccacgacacc gaaacggtca
     1741 acgtggaact gaagaaggag aagaaaggac cggtcacggt cattcacctg gactatcgta
     1801 caggcgctga ggtcggcgcg gaccaactcc ccgcacgcaa aaaggtacac gaagcgcatc
     1861 gttccaaatg gtcatcgcgc ggcctgcgtg ttcatcactc ggaatgctac gatgaaacca
     1921 gcggcaccac catctccctg gcctacgacg acaagctctt caagaacgcc atgggcggcc
     1981 ggatgacgaa cctgaccgtg accgccagcg gtggccatgc gtggagcacg agcttcgagg
     2041 gcaaggtggt ggcccacatg atccatgata tgctaccggc agcggaaggc ccggtgatcc
     2101 tgaacgtgct caccgcccat ggcaaggtgc tcgtggtgta cgagggcatc acctgtttgg
     2161 acctggccac cggcaaggtg ctgtggaaca ccaccttcga ctataccacc accagtgtcg
     2221 ggctcaaggc gaagcaggag atcggccgcg cagccatgcc cttggtcacc gcggatggcg
     2281 tgtacatcgc cgacctgagc aagggacaac gcaacatgaa gaagttggac agcaacaccg
     2341 gcgcagtgct ctggagcgcg gacaagctcg atggcgacga catcgtatcg gaactgctga
     2401 tcgcgggcgg caacttgatc gctcgcttcg gtggtgtggt acggcacgag atgttcgttc
     2461 cgaatccgaa cggcggggtg ggtgatggta cctacaaagt ggaatacgag ttcgaaggca
     2521 ggaccggcct tcgtgcatac aatgcgacca ccgggcaggc cgtgtggaac acttccgaca
     2581 tggagttggc ggacaatttc gccaagagcg agtgcaacat cctgagcgac ggcggacaga
     2641 tccacgcgct cggtgagaag aacatctatc tctttgatgc agctaccggc aaggtgacga
     2701 agcagaccga atacaacagc aagacgatcg ggaaggccag gtcgtactat gcgtacgatg
     2761 ggaaattcct catcgaaggc gaaaaaggca tcgctgccgt ggacaaggac ctcgcgctga
     2821 agtacgccac caacacgggc aaatgcctga tgtcggaaat gcgcggggat gccttcatcg
     2881 tttggaccgg caaggaggcc accgaccgca aggagttcat ccgcttcgat ccgaccaccg
     2941 gcaccatcat gggcaagctc gagggttgct atcgcccccg gttcgacctc acgggcgacc
     3001 gcttcgtccg gttcgatgat gaaaaggtga tcatgtaccg cacgaactga gcttcgtcgc
     3061 tcagggcagt tgggcaggtc tgatgttgtt cggggtggta ctgcccacgt tcagcaggat
     3121 gggatcacgg tcgttcccgc cacccgtgta cttcacctgt ccgttcatgt tcacatcgcg
     3181 ggtgctgtaa acgcctggga ccacgttgtt cggagtggta ctgccaacgg tcaccaggat
     3241 cggatcgcgg tcgtttcctc cgccggtata tttgatgatc ccgttgccag tggcatcgcc
     3301 gctccacaag gccctgcgtc cgaacgtcgt ggcccgggcg ttggtaccgt aggtgggcgt
     3361 gctcggcaca cgcaggtcga gcgccgcgcc ggaagcagag agtgccagcg gatcgtgtgt
     3421 catcacgccc aggtggttac gatgtttcac gcacaggtgg tagtcgccgg ccagcgcgtt
     3481 gaacgagatg ggtgaggcgc cgttcgtgcc gacgatcgtg ccgttgttct tgatcagccc
     3541 gcagcgggtc tggagcacga tggaaggatc gtccttgtcg cgcagttcca cgaagatcca
     3601 atccaccacc tggccgtaga gtatggtgag gaccatgggg ttgatcgttt cgcccccgct
     3661 gccgacgtgg ttgtaaccca aggctgtgaa tggttcggtg agggggataa ggttgttcgt
     3721 gcgcagtgca tcgctcatgg tggtggcgct ggtgagcggt ccttgcagga aggcgctcaa
     3781 ggtgaccggc aggttctgca ccaccggcgc ggtcacatgc accgcctgca ggaacggtgc
     3841 gctccaaccg ccgtcgctac cgagcacacg cacaccaagc aggtgcggtc cggcgggcag
     3901 ttcttccgat gcgttctgca cggccacttc gagcacttca tcgaagtcac catcgagcgc
     3961 ggagagcgga ataccgctgc ccgcaccggg atccgtatcc cagtagtatt cgccgctgat
     4021 caccgcgacg ggtcgtgcag ttaccggggc gctcacg
//
LOCUS       DAOQOC010000112         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10056_length_4054_cov_2.746187, whole genome shotgun sequence.
ACCESSION   DAOQOC010000112 DAOQOC010000000
VERSION     DAOQOC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10056_length_4054_cov_2.746187"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1375
                     /locus_tag="PL106_02805"
     CDS             266..1375
                     /locus_tag="PL106_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32027.1"
                     /translation="MNTGPNTLILALFLLGTTQGIAQTTIADGNWSDPSTWGGVPPFG
                     TGNVVIGHHVVLDLDYGHSSGSLTVNPGGSLVGNSDMRAFALNYPGGTATMTVNGTFD
                     VARVYLASNTVSNGGAFHADSLLCSATLTNSLGAGMVADQFRIDPGGTLNNAGSVETV
                     NFLNISTVVNSGSVSASDFANSKDFTVANTGVMNIAFDFSNIDTLGGPAIFTINGTVL
                     VGNDWHNGGQMNGSGRFCINHNTWNSGALTGTFDFCDLTGGDVDLNTGTVAGSITYCT
                     TPCDVGTGEGAEEVLVDIFPDPFSSSATVRTGRYLHNAAISVVDGLGRTVLRMSNITG
                     RTATLHRDGLPSGPYLLRLTQEGQVLATEKIIIDD"
     gene            1580..3160
                     /gene="bshC"
                     /locus_tag="PL106_02810"
     CDS             1580..3160
                     /gene="bshC"
                     /locus_tag="PL106_02810"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNA32028.1"
                     /translation="MLRHPIPYRATGRFSPIVSDHVEGEAALSEFYAHRPDREGLSAA
                     MRTRTFDPATRMVLCDVLRDQYHGIAVGEAVRANLEALRRNDVLTVTTGHQLCLFTGP
                     LYVPFKILNVIRLARELSTDEHNVIPVFWMATEDHDRAEIDHAWLDGAKVEWPGAPAG
                     AVGRLPLSGIDATVEQAGRVLGIGPHADELRALLNECYRPEHTLAHATRLFVHALFGR
                     FGVVIVDGDDPRLKSVFAPMMREEILNGVTSRAVHYANERLRPRYQPQAHARELNLFL
                     LQGLRRERLEPQGVGFVAHDTGTYWSVQALLDRLDAAPEEFSPNVLLRPLYQETILPN
                     IAYVGGGGELAYWLQLRWVFQAMRVPMPPLVLRTSAAFISTKLADKWRASGLSIADLF
                     TPKAELEKRIALEHATFSTSLEAERAEHARLFGALAERVTAADPTLEASVRAKEAFSR
                     KGLDLIERRLVRAAKRQQDDRLRQLGEVHDALFAHGLAERRDNFMSYYAAKGPALFDE
                     WLDALDPLDPRFSVLEGD"
     gene            complement(3274..>4054)
                     /locus_tag="PL106_02815"
     CDS             complement(3274..>4054)
                     /locus_tag="PL106_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32029.1"
                     /translation="RMDRYVGTPSATVTLGWNTATNCGVFDPATVHLAKWDGTMWRDR
                     GSPGTGSVALGTVSTTAPIIESNFTGGGWWTLATVSEDNPLPIELLSFDAHPEGSMVR
                     LDWHTATERDNDFFTIERSANGHDFEDLARVEGAGNSTAVLHYTDLDRWPLPGTSFYR
                     LRQTDFDGASTVSTIVTVRMPGKGPDDLVILADGDHITGLHDFVAGSTCDVLDMTGRV
                     VWQGRTAVDGRTDVPFAGLSGGAYVLRVSDGTRSASAPFVR"
BASE COUNT          760 a         1242 c         1291 g          761 t
ORIGIN      
        1 cgggtgcgga aaggtacagg tgggatccca gggtcgatgg acctggaacg cggagacgga
       61 ctccattcat cggccatgat cctcgtggct cccagcgacc gatagacccc aacatgaccg
      121 cgtgcatcct gttccgcagt gcccagcctc cgaaaaagac gtgtatcgcc atgaccgcac
      181 gcggtgaaag cggccccatc gctcctgacc ggagcatcat aacttcaccg acagccgaac
      241 ggcccacaat aagaacatcg ggatcatgaa taccggaccg aatacattga tccttgcgtt
      301 gttcctgctt ggaacgaccc aaggcatcgc gcagaccacg atcgcggacg gcaattggtc
      361 agacccgtcc acttggggcg gtgtcccgcc gtttggaacc gggaacgtgg tgatcggaca
      421 ccatgtcgtc ctggatctcg attacggcca cagctccgga tcccttacgg taaacccggg
      481 cggatcgttg gtgggcaact cggacatgcg ggcattcgca ctcaactatc cgggaggtac
      541 ggccaccatg acggtgaatg ggacgttcga tgtggcaagg gtgtatctcg cctcgaacac
      601 ggtgagcaat ggcggtgctt tccatgcgga cagcctgttg tgctcggcaa cgctcacgaa
      661 cagccttgga gcgggaatgg ttgccgatca gttcaggatc gacccggggg gcacgctgaa
      721 caatgcgggc tcggtggaga cggtgaactt cctgaacatc tcgaccgtgg tcaacagcgg
      781 gtccgtttcg gccagcgact tcgccaactc caaggacttc acggtggcca ataccggggt
      841 catgaacatc gcgttcgatt tctccaacat cgacacgctg ggggggccgg ccatcttcac
      901 catcaatggg accgttcttg tcggcaacga ctggcacaat ggtggtcaga tgaacggttc
      961 cggtcgcttt tgtatcaacc acaacacatg gaattccggt gcgctgaccg ggacctttga
     1021 tttttgcgat ctgaccggcg gcgatgtgga tctgaatacc ggaacggtcg ccggatcgat
     1081 cacctactgc acgacacctt gtgatgtcgg gaccggagaa ggggcggaag aggtactggt
     1141 cgacattttt ccggatccct tctcatcatc ggccaccgtg cggaccggta gatatctgca
     1201 caatgcggcg atctccgttg tcgatggcct tggccggacc gtgttgcgaa tgagcaacat
     1261 cactggccgg accgcgacgt tgcatcgtga tggtttgcca agcggccctt acctgctccg
     1321 attgacgcag gagggccagg tcctcgcaac ggaaaagatc atcatcgatg actgacacgc
     1381 gcagaggatg gcgctccacg gccaagaggc cttcggacgt gcgactttac cacgcgcatc
     1441 atgcttcgtg ctgtccgccg gccgcacgac tggcgacggt gatgatccca tgcttggtcg
     1501 cggggatccg aagtggtccg accgggtact ttcgtcggcc atgatccgat gctgaatcgt
     1561 tcgggtcatt cccgttcgca tgctgcgcca tccgatcccc taccgcgcca ccggccgctt
     1621 ttcgcccatc gtgtcggatc acgtggaagg ggaggctgcc ctcagcgagt tctacgccca
     1681 ccggcccgat cgcgaagggc tctcggccgc catgcgcaca aggaccttcg accctgccac
     1741 tcggatggtg ctgtgcgacg ttcttcgcga ccagtaccat ggcatcgcgg tgggtgaggc
     1801 ggtacgggcg aacctggaag ccttgcgccg gaacgatgtg ctcacggtga ctaccggaca
     1861 tcagctctgc ctgttcacgg gccctttgta cgttccattc aagatcctca acgtgatccg
     1921 gctcgcccgg gagctgagca cggacgaaca caacgtgatc ccggtgttct ggatggccac
     1981 cgaggaccac gatcgggccg agatcgatca tgcgtggctg gacggtgcga aagtggagtg
     2041 gccgggtgcg cctgcggggg ccgtagggcg tttgccgctc tctgggatcg acgccactgt
     2101 ggagcaggcc gggcgagtac tgggcatcgg accccatgcg gacgagttgc gcgcgctgct
     2161 gaacgaatgc taccggccgg agcataccct ggcccacgcc acacggttgt ttgtacacgc
     2221 gctcttcggt cgcttcggcg tggtgatcgt ggatggcgat gatccacgcc tgaagtccgt
     2281 cttcgcgccc atgatgcgcg aggagatcct gaacggggtg accagccgcg ccgttcacta
     2341 tgcgaacgaa cggttgcgcc cgcgttacca gccgcaggcc catgcccgcg agttgaacct
     2401 tttcctgctg caagggctca ggcgcgaacg cctcgaacca cagggtgtgg ggttcgtggc
     2461 gcacgatacc ggtacctatt ggtcggtaca ggcgttattg gaccggcttg atgcggcacc
     2521 ggaggagttc tcgcccaatg tgctactacg gccgctgtac caggagacca tcctccccaa
     2581 catcgcgtat gtgggcgggg gcggcgagct ggcgtactgg ctgcagttgc gctgggtctt
     2641 ccaagcaatg cgcgttccaa tgccaccgtt ggtgctgcgt acgtcggccg ccttcatcag
     2701 caccaaactc gctgacaagt ggcgggcttc gggcttgtcc atcgccgatc tgttcacacc
     2761 gaaagcggag ttggagaagc ggatcgcctt ggagcacgcc accttcagca cctcactcga
     2821 agcggagcgc gccgaacatg cgcggctgtt cggagcactg gcagagcggg taacggccgc
     2881 cgatcccacc cttgaagcca gtgtacgggc caaagaagcc ttttccagga aagggttgga
     2941 cctgatcgag cgccgcttgg tgcgagccgc caaacgccag caggacgacc gtctccgcca
     3001 gctgggggag gtacacgacg ccctgttcgc ccatggcctc gcggagcgac gggacaactt
     3061 catgtcgtat tacgcggcaa aggggccggc cctgttcgat gaatggctcg atgcattgga
     3121 tcccctggat ccccgtttca gcgtattgga gggcgattga cacgttcggc cgtcgccgcg
     3181 agaggtccac aacaggactg ttgaaccggc ggaacaggtt cccccgggcg gtcaatggga
     3241 atggagaagg tggttcccgg gggtcaggtc ggctcagcgc acgaagggtg cgctcgcgct
     3301 ccgggtgcca tcgctgacgc gcaagacgta cgcaccgccg ctcagtccag cgaaaggaac
     3361 atcggtccgg ccgtccaccg cggtgcggcc ctgccacacc acacggccgg tcatgtcgag
     3421 cacatcacag gtgctgccgg ccacgaagtc gtgcaggccg gtgatatggt ccccatctgc
     3481 aaggatcacc aggtcgtcgg ggcccttgcc cggcatgcgt acggtaacga tcgtgctcac
     3541 ggtactggca ccatcgaagt ccgtttggcg caagcggtag aagctggtgc cgggcagggg
     3601 ccagcggtcc agatcggtgt agtgcagcac agcggtgctg ttccccgctc cttccacccg
     3661 ggccaggtcc tcaaaatcgt gcccgttggc gctgcgctcg atggtgaaaa agtcgttgtc
     3721 tcgctcggtg gccgtgtgcc agtccagccg gaccatcgat ccctcgggat gggcatcaaa
     3781 ggagagcagt tcgatgggaa gcggattgtc ctccgatacc gtggccaggg tccaccagcc
     3841 tccaccggtg aaattggatt caatgatcgg ggcggtggta ctcaccgtgc cgagcgccac
     3901 cgagcccgta cccggtgaac cgcgatcgcg ccacatggtg ccgtcccact tggccagatg
     3961 cacggtagca ggatcaaaaa caccacagtt ggtggccgtg ttccagccga gtgtcaccgt
     4021 ggccgagggt gtgcccacgt agcggtccat gcgg
//
LOCUS       DAOQOC010000113         4048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10088_length_4048_cov_3.410969, whole genome shotgun sequence.
ACCESSION   DAOQOC010000113 DAOQOC010000000
VERSION     DAOQOC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10088_length_4048_cov_3.410969"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1291)
                     /locus_tag="PL106_02820"
     CDS             complement(164..1291)
                     /locus_tag="PL106_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNA32030.1"
                     /translation="MPSSSTRTIVTGAERMERYLPFLQGKRVAVASNHTGTVGRSHIV
                     DTLLSAGVTVVKVFAPEHGFRGTADAGEHVTNEQDARTGLPIVSLYGRNKKPTAQQLA
                     DVDVVLFDIQDVGVRFYTYISTLHYVMEACAENGKALIVLDRPDPNGFYVDGPVLDTA
                     FRSFVGMHPVPLVHGMTIGEYANMINGEGWLKLGVKCDLKVITCIGYDHTVHYDLPIR
                     PSPNLPNMAAVYLYPSLGLFEGTMVSVGRGTEQPFQCIGFPGWSKGDFAFTPRSMPGA
                     KDPPYKDTTCNGLDLREYGEFITRLEPRVHLEWLIATYGAARDRSRFFTPFFDRLAGG
                     KALREAVMRGESEEGIRASWEPGLKKFGAVRKKYLLYKDVH"
     gene            1329..2927
                     /locus_tag="PL106_02825"
     CDS             1329..2927
                     /locus_tag="PL106_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32031.1"
                     /translation="MRSTSKAMVVQARPTFVREHMGFAHFIARRILVDKDRQDRLSRP
                     IVLIAVLGIVIGMAVMILTVGISTGFQREVRAKVTGAGSHIEIVPLGGGDTRDSERMT
                     IEQPFYPWLDTVPGVARISVFALKPGIVETENEIEGVVVKGLGADHDWTFLRRHLVRG
                     GILPIPDTAGMASALISNWLAKRLRQGTGDTVTIYLVRDSDEPRARKFLVRGIYETGI
                     EKIDQQLVFVDIAHIQRFAQWGLRAEMSVYDTVSPKGIRIEGHAFGGVPPYEYEWPGT
                     ALKGRGPHWISTSTDTTITLVTHDIRGTIPDTAWIRIAPGMHAPGQRDILTRTLANIT
                     RGGSGGSYGAYCGGFEVALNDHRDLMAMDDLIYNEFLDPDQRTITALQRFPEIFTWLD
                     LLDTNVVVVIVLMVVVAIINMTSALLIIILERTNMIGTLKALGTGNGTIRRIFLIDAA
                     YILGIGIILGDILGIGLAMLQKGFGIVRLPVESYYVDTVPVALDPLPIALLNAGTLLV
                     CVLALMIPSMLVTRIAPARAVRFA"
     gene            2978..>4048
                     /locus_tag="PL106_02830"
     CDS             2978..>4048
                     /locus_tag="PL106_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNA32032.1"
                     /translation="MTRIHDNILSTIGNTPMVRLNRITKDIPATVLAKVETFNPGNSI
                     KDRMAIKMVEDAEKAGLLKPGYTIIEGTSGNTGMGLAIAAIIKGYKCIFTTTDKQSKE
                     KVDILRAFGAEVIVCPTNVDPEDPRSYYSVSSRLVNETPNSWKANQYDNPSNAQAHYE
                     TTGPEIWEQTGGKIDHFVVGVGTGGTICGTAKYLKERNPKLKVWGIDTYGSVFKKLKE
                     TGEFDKNEIYPYITEGIGEDFVPANVDMGLIDHFEKVTDRDAAIYTRRIVKDEAIFVG
                     NSAGAAMAGLLQLKDNFKKGEVVVVIFHDHGSRYVGKMFNDDWMRERGFLVEEKPTAG
                     DLLKGKDLALLSVEAEEPVLNAI"
BASE COUNT          836 a         1223 c         1247 g          742 t
ORIGIN      
        1 aatacggtgc tgcaacttga ccggcgcgtg gccccgggca gctacctggt ccgcgtgatc
       61 gttgaccatc ggaactggac cgagcgcctg gtgatccagt agcgggccgg tccacaagga
      121 gaagccatcc gccccgggcg ggtggcttct tcgctaccgg tgctcagtgg acatccttgt
      181 acaacaggta ttttttgcgc acggcaccga atttcttgag gccgggttcc caggaggccc
      241 ggatcccttc ctcgctctca ccgcgcatca cggcctcccg cagggccttc cctcccgcca
      301 acctgtcgaa gaatggcgtg aagaaccggc tcctgtcccg ggccgcgccg tatgtcgcga
      361 tgagccactc cagatgcacg cgtggttcca gccgggtgat gaactccccg tactcgcgca
      421 gatcgaggcc gttgcacgtc gtgtccttgt acggtgggtc cttggcccct ggcatggagc
      481 ggggtgtgaa tgcgaagtcc cctttcgacc atcccgggaa cccgatgcac tggaacggtt
      541 gttccgtgcc gcgtccaacg ctcaccatgg tgccctcaaa aagccccaat gaagggtaca
      601 aatacaccgc ggccatgttc ggcaggttgg gcgatggccg gatcggcagg tcgtagtgga
      661 ccgtgtgatc gtagccgata caagtgatca ccttcaggtc gcacttcacc cccagcttga
      721 gccagccctc accattgatc atgttcgcgt actcgccgat cgtcatgcca tgtacgagcg
      781 gtaccggatg catgccgacg aaggaacgga aggccgtgtc caacaccggg ccgtccacat
      841 agaacccgtt cgggtccggt cggtccagga cgatgagcgc tttgccgttc tcggcgcacg
      901 cttccatcac gtagtggagg gtgctgatgt aggtgtagaa gcgcacaccc acgtcctgga
      961 tatcgaaaag aaccacatcc acatcggcga gttgctgggc cgttggtttc ttgttcctgc
     1021 cgtagagtga aacgatgggc aggccggtgc gtgcatcctg ctcgttggtc acgtgctcgc
     1081 ccgcgtccgc agtgcccctg aacccgtgct ccggcgcaaa aaccttcacc accgtcaccc
     1141 ctgcactcag gagcgtatcc acgatatgcg aacgaccgac cgtgcccgta tggttgctgg
     1201 ccacggctac gcgctttcct tgaaggaacg ggaggtagcg ttccatgcgt tcggcgcccg
     1261 taacgatggt tcgcgtactg cttgaaggca tcaccatccc ggactgatca tcctgtgcgc
     1321 cgcacgacgt gaggagcacc agcaaagcga tggtggtgca ggcccggcct acattcgtcc
     1381 gcgaacacat ggggttcgcg catttcatcg cccgccgtat cctggtggac aaggaccggc
     1441 aggaccgcct ctcacggccc atcgtactca ttgcggtgct gggtatcgtc atcggcatgg
     1501 cggtgatgat cctcacggtg gggatcagta cgggtttcca gcgcgaggtc cgtgccaaag
     1561 taacgggtgc cggatcgcat atcgagatcg ttccgcttgg cggaggcgac acacgcgatt
     1621 cggagcgtat gaccatagag caaccattct atccctggct ggataccgtg ccgggggtgg
     1681 cacgcatctc cgttttcgca ctgaagcccg ggatcgttga aacggagaac gagatcgagg
     1741 gcgtggtggt caagggcttg ggcgccgacc acgactggac cttccttcgc cggcacctcg
     1801 tgcgtggcgg aatattgccg atcccggaca cggccggcat ggcctccgca ttgatcagca
     1861 actggctggc caaacgactt cggcagggca caggcgatac cgtcaccatc tacctggtgc
     1921 gcgacagtga cgaaccacgt gcccgcaaat tccttgtgcg cggtatttat gaaacaggca
     1981 tcgagaagat cgatcagcaa ctggtattcg tggacatcgc gcacatccag cgattcgcac
     2041 aatggggttt acgtgccgaa atgagcgtgt acgatacggt cagcccgaag gggatccgga
     2101 tcgaaggcca tgcgttcgga ggcgttccgc cgtacgaata cgagtggcca ggtaccgcac
     2161 tcaagggcag gggtccgcat tggataagca ccagtaccga caccaccatc acgctggtga
     2221 cccacgatat ccgggggacc atcccggaca cggcatggat caggatcgca ccgggcatgc
     2281 atgcaccggg ccagcgggat attttgaccc gcacgctggc gaacatcacc cgcggcggca
     2341 gtggcggcag ctacggcgcc tattgcggtg ggttcgaagt ggccctgaac gaccaccgcg
     2401 acctgatggc gatggacgac ctgatctaca atgaatttct tgacccggac cagcgaacga
     2461 tcaccgcttt acagcgcttc cctgagatct tcacatggct tgacctgctc gataccaatg
     2521 tggtcgtggt gatcgtgctc atggtggtcg tggcgatcat caacatgacg agcgcattgc
     2581 tgatcatcat cctggagcgc accaatatga tcggaacact caaggccctg ggcacgggca
     2641 atggcacgat ccgccggatc ttcctgatcg atgccgcgta catcctgggt atcggcatca
     2701 tcctcggcga catattgggg atcggcctgg ccatgctgca gaagggcttc ggcatcgtgc
     2761 gtctccccgt tgaaagctac tacgtggata ccgttccggt ggccctggac ccgctgccca
     2821 tcgccctgtt gaacgcgggc acgctgctcg tatgcgtact ggccctcatg atccccagca
     2881 tgctggtaac acgcatcgcg ccggccaggg ccgtccggtt cgcataggcc tccgccatga
     2941 gcgatcggtc gtgccagcga gcggtacatt cgcgacgatg acgcgcatcc acgacaacat
     3001 actctccacc atcggcaaca cgccgatggt gcgcttgaac aggatcacca aggacattcc
     3061 cgccacggta ctggccaagg tggagacctt caatccgggc aacagcatca aggaccgcat
     3121 ggccatcaag atggtggagg acgcggagaa ggcggggctg ctgaaacccg gctacaccat
     3181 catcgaaggc accagtggca acaccggcat gggcctggcg atcgccgcga tcatcaaagg
     3241 gtacaaatgc atattcacca ccaccgacaa gcagagcaag gagaaggtgg acatcctgcg
     3301 cgcgttcggc gcggaggtca tcgtttgccc cacgaacgtg gatcccgagg atccccgctc
     3361 ctactattcg gtctcatcgc gcctggtgaa cgaaacgccg aacagttgga aggccaacca
     3421 gtacgacaac ccgagcaacg cacaagcgca ttacgagacc acgggacctg agatctggga
     3481 gcagacgggc ggcaagatcg accacttcgt ggtgggcgtg gggaccggcg gtacgatctg
     3541 cggaacggcg aaatacctga aagagaggaa cccgaagctc aaggtgtggg gcatcgatac
     3601 ctatggatcc gtattcaaga aactgaagga gaccggggag ttcgacaaga acgagatcta
     3661 cccgtacatc acggaaggga tcggcgagga cttcgtgccg gcgaatgtgg acatgggcct
     3721 gatcgatcac ttcgagaaag tgaccgaccg tgatgcggcc atctacacgc gcaggatcgt
     3781 gaaggacgag gccatcttcg tgggcaacag tgcgggtgcg gcgatggccg gcctgctgca
     3841 gctgaaggac aacttcaaga agggcgaggt ggtggtggtg atcttccacg accacggcag
     3901 ccgctatgtg ggcaagatgt tcaacgacga ctggatgcgt gagcgcggct tcctcgtgga
     3961 ggagaagccc acggccggcg accttttgaa gggcaaagac ctcgcgctgc tgagcgtgga
     4021 ggccgaggaa cccgtgctga acgcgatc
//
LOCUS       DAOQOC010000114         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10097_length_4046_cov_3.809321, whole genome shotgun sequence.
ACCESSION   DAOQOC010000114 DAOQOC010000000
VERSION     DAOQOC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_4046_cov_3.809321"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PL106_02835"
     CDS             complement(<1..543)
                     /locus_tag="PL106_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNA32033.1"
                     /translation="MFKMADRQDRIAEHQRGSTFPTRTACATFLTFTLSTAVVLLTGT
                     LLVGCGSSPDEEAEDADQGRYTATGPLLTDTVVDQTYVCQIHSIQHIEVRALEKGYLQ
                     DILVDEGQHVKEGQLMFRIRPVLYQAEVQQAAAEAEFAEIEFRNTKALADSNVVSPNE
                     LAMARARYEKAKAELAAAQAH"
     gene            complement(716..1033)
                     /locus_tag="PL106_02840"
     CDS             complement(716..1033)
                     /locus_tag="PL106_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32034.1"
                     /translation="MHSEKNYWAKRVTIACLLILGMQFCFQRTVQASLLGTQQVDMIS
                     LGVKDPAGKPGVHAELEPPHAETWTITVPTGTEAPFAEPSGEYPPAVPAVLPAAGLYP
                     SAP"
     gene            complement(1194..3203)
                     /locus_tag="PL106_02845"
     CDS             complement(1194..3203)
                     /locus_tag="PL106_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32035.1"
                     /translation="MAGDFTATGNGTTQLAYLARWDGSAFQPVGTGVSGKVQDLLSMP
                     NGLWMAGSFRYTADSTVALNQFAVFNGDSFAPLIPDFTGFIPVGDTFDHLMIPVGGIG
                     VLAMANDRLVYKDGEWKLTWLPKLYCALEYQGKEYAGGNSSIYSMLCRKLALRELLPG
                     NGPLPIDVAGVRSWVGADGVLFLDQEEQMPSFTPWSEGGASVIYAHGQYVVGYVGDSA
                     FTSSFSPYGNHDCDGKGYDPGPRCTERDLAFRERYSRTWPIDIGQLWEHAAHWNDPGY
                     TVPEVLANWPAHGDPGNGEPAQLVPFQDMDGDGLYEPDQGDMPVIRGDKAVLTVVSDQ
                     IADTMPGMRPSMFDSRILVHGFDNGPEALHHTLLVNYAFTNRSDRTYDSVVVAMTTDP
                     DLGCAGDDFIGCDPELDMFYVYNADATDEECNGQPGFGLHPPAIGIVGLSAPMRAFIY
                     FNHEGAACCNDPQSAQHAANYAEGIWKDGSPLINPITNDTTRFMFSDYPDVPGSYHET
                     ALIDPDRRGTASFGPFFNVAPGETICFDMAFVFARDTTQDNIQNTRVLQQKVIALKQW
                     YAQQAYGCGEYPDVGIQAPVEDGAALTLYPNPAQGQVTAARTDIREAWHVSVRSVTGE
                     LVHQALWPAGTRTWGFTTDALAEGVYMVRFSGDPGQVVRRLVVAR"
     gene            complement(3104..3736)
                     /locus_tag="PL106_02850"
     CDS             complement(3104..3736)
                     /locus_tag="PL106_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32036.1"
                     /translation="MKTPFLLALLAPMAAVAQRLIPSDVQIAGSVYALAEFDDKLIIG
                     GDFDAVNGQPISNIVAWDGGTGFDDLGAPMPSGQVTAVLPYANGVVFGGLAPGDYRRV
                     FHWNGDTTTVLGTPGSGRINCFTEFNGDLIVGGSFFTMDGVVVNRIARWDGMGWTSMG
                     TGLNQMVRSVLVHQGSCTWPGISPPQGMARHNWPTWPGGTGVLSNPSVPG"
BASE COUNT          778 a         1226 c         1276 g          766 t
ORIGIN      
        1 gtgcgcctgg gccgcggcca gttcggcctt ggccttctcg tagcgcgccc tggccatggc
       61 cagttcgttc ggcgatacca cgttgctgtc ggccagggcc ttcgtgttgc ggaattcgat
      121 ctcggcgaac tcggcttcag cggccgcttg ctgcacctcg gcctggtaca gcaccgggcg
      181 tatgcggaac atgagttgcc cttccttcac gtgctgtccc tcgtccacca ggatgtcctg
      241 caggtagcct ttctccaggg cgcgcacttc gatgtgctgt atggagtgga tctggcacac
      301 gtaggtctgg tccaccacgg tgtcggtcag caacggcccc gtggccgtgt atcggccctg
      361 gtccgcgtct tccgcttctt catcagggct ggagccgcac ccgaccagca gggtgccggt
      421 caacagcacc accgcagtgg aaagagtgaa cgtgaggaat gttgcgcacg cggtgcgggt
      481 ggggaacgtg ctgccacgct ggtgttccgc gatccggtct tggcgatcgg ccatcttgaa
      541 catgtcggaa ggatgtggaa gttgagtacc ggtgcctccg gctggacccg agcggttgtt
      601 gccgcgcccg ggaaatgaag aagttgaagt acggagtgcg ttcacggatc gctggcgaca
      661 atggccggat gcgatcgttg aacaggtccg gcgggaatgc cgggccgggc aagccttacg
      721 gcgcggacgg atacagccct gccgctggca gcaccgctgg cactgccggg ggatattcgc
      781 cagatggttc agcgaagggg gcctcggtac ctgttggtac cgttatcgtc catgtttccg
      841 cgtggggcgg ctcaagttcg gcgtgtacac ccggtttgcc tgccgggtcc ttcaccccga
      901 ggctgatcat atccacctgc tgcgtgccca gcagagatgc ctgcacggtc cgttggaagc
      961 agaactgcat ccccaggatg agcagacagg cgatggtcac ccgcttcgcc cagtagttct
     1021 tttcggaatg catggtggat ttgcggctgc gaacttggta tgatacgcac gtggtcggca
     1081 agaccttaac aagaatttta acgaccgtgc ggatcgatcg acggatcggt gccgtagccg
     1141 atgcgacatg gacaacgaac ggatcgtgcg tgccggagcg gagtggcggc accctaccgc
     1201 gccaccacca accgccgcac aacctggccc ggatcgccgc tgaaccgcac catgtacaca
     1261 ccctcggcca gcgcatcggt ggtgaagccc caggtgcgcg tgcccgcggg ccacagcgct
     1321 tgatgcacca gttcaccggt gaccgagcgg acgctcacgt gccatgcctc gcggatatcg
     1381 gtgcgcgcgg cggtcacctg gccctgcgcg gggttggggt acagggtgag cgctgcgcca
     1441 tcctccactg gcgcctggat cccaacatca gggtattcac cacagccgta ggcctgctgg
     1501 gcgtaccact gcttcagcgc gatcaccttc tgctgcagca cccgcgtgtt ctggatgttg
     1561 tcctgcgtgg tatcccgtgc gaagacgaag gccatgtcga aacagatggt ctcgccgggc
     1621 gccacgttga agaaggggcc gaaggaggcc gtgccacgac ggtcaggatc gatcaaggcc
     1681 gtttcgtggt aactgcccgg cacgtccggg tagtccgaga acatgaagcg cgtggtatcg
     1741 ttcgtgatcg ggttgatcaa tggagaacca tccttccaga tgccttccgc gtaattggcc
     1801 gcgtgctgcg cggactgcgg atcgttgcag caagccgcgc cttcatggtt gaagtagatg
     1861 aaggcccgca tcggcgcgct gaggcccacg ataccgatgg ccggtgggtg aagaccaaaa
     1921 cccggctgac cgttgcattc ttcatcggta gcatcggcgt tgtacacgta gaacatatcc
     1981 aattcggggt cacagccgat gaaatcatca ccggcgcagc cgagatccgg atcggtggtc
     2041 atggccacca ccaccgaatc gtaggtgcgg tccgaacggt tcgtgaatgc gtagttgacc
     2101 agcagggtgt ggtgcagtgc ttcaggccca ttgtcgaatc cgtgcaccag gatccgcgag
     2161 tcgaacatgg agggtcgcat gccgggcatg gtatcggcga tctggtcgct caccacggta
     2221 agcaccgcct tgtcgccccg gatcacgggc atgtcgccct ggtccggctc gtagaggcca
     2281 tcgccatcca tgtcctggaa aggcaccaat tgtgcgggct cgccattgcc ggggtcgccg
     2341 tgggcgggcc aattggccag cacctcaggt acggtgtatc cgggatcgtt ccagtgcgcg
     2401 gcgtgctccc acagttggcc gatgtcgatg ggccaggtgc gtgaataccg ttcgcggaac
     2461 gccaggtcac gctcggtgca acgcggcccg gggtcatagc ctttcccgtc acaatcatgg
     2521 tttccgtatg gtgagaagga cgacgtaaag gccgaatcgc ccacatatcc caccacgtat
     2581 tgcccgtggg catatataac cgaagcgccg ccctcggacc atggggtgaa cgagggcatt
     2641 tgctcttcct ggtccaagaa cagtacacca tcggcaccca cccatgatct gactcccgca
     2701 acatcgatcg gaagagggcc gttaccgggc agcaactcac gcagggccaa ttttctacag
     2761 agcatagagt aaatactgga attgcctcca gcgtactcct tgccttggta ctcgagcgcg
     2821 cagtacagct ttggcaacca tgtcaatttc cattccccgt ccttgtatac gagccgatcg
     2881 ttcgccatag ccagaacacc gatcccgccg acagggatca tgagatgatc gaatgtgtca
     2941 ccgacaggta taaaacctgt gaagtccggg atcaatggag cgaagctgtc gccgttgaag
     3001 accgcgaact ggttcagggc cacggtactg tccgccgtgt atctgaacga gcccgccatc
     3061 cagagcccat tgggcatgga gagcaggtct tggactttac cgctcacccc ggtaccgacg
     3121 ggttggaaag cactcccgtc ccaccgggcc aggtaggcca gttgtgtcgt gccattccct
     3181 gtggcggtga aatccccggc cacgtacagc tgccctgatg gaccaagaca ctccgaacca
     3241 tttggttgag tcctgtgccc atggacgtcc aacccattcc gtcccaacgc gcgatccgat
     3301 tcacgacgac cccgtccatg gtgaagaagg agcctcctac gatcagatcg ccattgaact
     3361 cggtgaagca gttgatgcga ccggagccgg gcgtgcccag taccgtggtg gtgtcgccgt
     3421 tccagtggaa tacccggcgg tagtcgccgg gagcgaggcc accgaacacc acgccgttcg
     3481 catagggcag gaccgcagtc acctgaccgc tcggcatggg cgcacccaga tcatcgaagc
     3541 cagtgccgcc gtcccaagcc acgatgttgc tgatgggttg accattgact gcgtcaaaat
     3601 ccccaccgat gatgagtttg tcatcgaact cggcgagcgc atatacgctg cccgcgatct
     3661 gcacatcgga cggaataagt cgctgcgcaa cggccgccat gggtgcgagc aacgcgagga
     3721 ggaaaggtgt cttcatggct tcgggttcat cttcctggta cacgaaggaa tcgaaaccgg
     3781 tcggaaggcg gacgcggaga acgaggaacg gcggggacag gcagcgaccg ggatgctggg
     3841 gtaggaagcg ggaacggatc gcatcaccgg ctgcgtcatc ccgacggccg ggcaaccccg
     3901 ttccggtaca tgcgaacgac gacctgccga aggacctgct gcggaagatc gtccgcgaag
     3961 acctccaact gtcgctggag gaattcaacg cagtggttga gcgggagggc tgagcaagcg
     4021 gggcgtccgt gttcataacc cttccc
//
LOCUS       DAOQOC010000115         4037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10132_length_4037_cov_4.811652, whole genome shotgun sequence.
ACCESSION   DAOQOC010000115 DAOQOC010000000
VERSION     DAOQOC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_4037_cov_4.811652"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PL106_02855"
     CDS             <1..445
                     /locus_tag="PL106_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32037.1"
                     /translation="LDQLAAVTAHKPRLHVHGTVNLAGSGAACSLHVVVGGELVDVQD
                     ITNQGRFRLDLSADEHIRLILVSTGHFPRVVEIRPARTVSGPVNHLDLCVVLTPNGLV
                     DGKRAAPLREKLIPGRDGAPDVIGYDRTPNSRPNSTPLPLLKRAS"
     gene            complement(525..2015)
                     /locus_tag="PL106_02860"
     CDS             complement(525..2015)
                     /locus_tag="PL106_02860"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNA32038.1"
                     /translation="MSVQVLEKRGRKNILVCSIGSAKEQADIHDLKERASRFIAQRTG
                     QQGLDLHLQDEQDRFDATFKSIGDVILLGPELVLGRLFEQIGFGAVPDEMFRHLVLSR
                     IMHPCSKLKTVRYLQEQRRTQMHVQEIYRYMDELSSTHKQQVEQISHAHSMRVLGGTL
                     SAVFYDVTTLYFEAEQPDDLRIPGYSKDGKHNDPQVLLGLLVGAGGYPLAYDIFKGNT
                     FEGHTMIPVIERFKQRFGAGSLVVVADAGLLSRANMDALHRAGHQFILGARLRGEKDA
                     TKQAILNNRYIQDAPQEILREDGLRLVVSYSQGRAAKDKHNRERALARLQRAVQGGKL
                     TKANINNRGYNKFLRMQGLLEVSIDNEKLKEDARWDGLKGYLTNTTIPAQELIDNYKH
                     LWAIEQAFRISKTDLRVRPVYHRLQRRIEAHICICFSAYQVYKELQRQLFQNQCGLSA
                     ERAIECLATVYGITIHDQTSGRSQTRILAKTQDQQLLLRTFGIDFG"
     gene            2328..3098
                     /locus_tag="PL106_02865"
     CDS             2328..3098
                     /locus_tag="PL106_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32039.1"
                     /translation="MNRIVTTALLSAVTVVAQAQTAINFNCNDCAGTPHDLFAELDAG
                     KVVVLAWVMPCAPCVGPSLTMRNIVQSYQASDPGRVIMYLCDDYGNTSCSALNGWMNA
                     NGLSGLTSFSNPAINMTDYGGPDMPMFAVVGGPDHVVFFREDGTVDVPAVQDAIAAAL
                     SATAITEESNLTGAPSIHPVPATDRAWIDIPLAHAARVTVDLYDLSGSLVRSVHNGPL
                     LPGDHRIGLDTREIPAGMYVIRVLAGEERVALNLVIAH"
     gene            3311..>4037
                     /locus_tag="PL106_02870"
     CDS             3311..>4037
                     /locus_tag="PL106_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32040.1"
                     /translation="MELNTNFQYIHSNSFYRGSERASEHTFDSFESKLQYFKVAYGAS
                     KNLTVSLEYSHYLLKKETGLNGDPATTYTCKGMGDLIIFPRYNMFHKETRSSSDEVTI
                     GLGYKVPLGAYNDSIGNVEPFSGQTFYVTKPTAVQLSSGAQDLIVHTFYHHGFLRSGL
                     NFFASALYIRKGWNPNGEKMGDFASLAVYGSRTFSKRYGITLQVRYEYADRMRINNNI
                     LMFGKPSNYFPEATGYCKWFFTPQ"
BASE COUNT          791 a         1249 c         1160 g          837 t
ORIGIN      
        1 cctggatcag cttgccgcgg taacggcgca caagccccgc ctgcatgtac atggtaccgt
       61 gaacctggcc ggcagtggtg ccgcatgttc cctgcatgtg gtggtcgggg gcgaactggt
      121 cgacgttcag gacatcacca accagggtcg cttccgtctc gacctgtccg ccgatgaaca
      181 catccgcctc atcctcgtga gcaccggcca ctttccgcgg gtggtggaga tcaggcctgc
      241 ccggaccgtc tccggtccgg tgaaccacct ggacctttgc gtggtactca ctccgaacgg
      301 cctggtcgat gggaagcgtg ccgcaccact gcgcgaaaag ctcatcccgg gtcgtgatgg
      361 ggctcctgat gtgatcggat acgaccgcac cccgaactcc cgaccgaatt ccacgccact
      421 gcccttgctc aagcgggcca gctgagcgga gcggttacct tgggggcgga ccgatcctgc
      481 ccatgcgccc gatcatttcc gcgctcctgt tttagccaaa tgggtcaccc aaagtcgata
      541 ccgaaggttc tgagcaggag ttgttggtct tgggtcttgg ccaggatcct ggtctgggag
      601 cggcctgaag tctggtcatg gatggtgatg ccatagaccg ttgcgaggca ttcaattgcc
      661 ctttcagcgg agaggccgca ttgattttgg aacagttgcc gctgtagttc cttgtagacc
      721 tggtatgcgg aaaagcaaat gcagatgtga gcctctatgc gccgttgcag ccggtgatat
      781 accgggcgta cgcgcagatc ggtcttggag atgcggaagg cctgctcgat ggcccagagg
      841 tgtttgtagt tgtcgatcag ctcttgggcc gggatggtgg tattggtcag gtatcccttg
      901 agcccgtccc aacgggcatc ctctttgagc ttttcgttgt ctatggagac ctccaacaga
      961 ccctgcatgc gcaggaactt gttatagccc cggttgttga tgttggcttt ggtgagcttg
     1021 cctccttgca cggcccgttg gagccgggcc aaggccctct cccggttatg cttgtccttg
     1081 gcggccctcc cttgggagta gctcaccacc agtctcagac catcttcccg gaggatctcc
     1141 tggggcgcat cttggatata ccggttattg aggatggcct gtttggtggc gtccttctca
     1201 ccacgcaggc gggctcccag gatgaactgg tgaccggctc tatgcaaggc atccatattg
     1261 gcgcggctca acaaccccgc atcggccacc accaccaatg aacccgcgcc gaagcgctgc
     1321 ttgaaccgct cgatcaccgg gatcatcgtg tgaccctcga aggtgttgcc cttgaagatg
     1381 tcgtaggcca acggatagcc gcccgcaccc accagcaggc ccaggagcac ctgcgggtcg
     1441 ttgtgcttac catccttgga atatccgggt atgcgcaggt cgtcgggctg ctcggcttcg
     1501 aagtacaggg tggtcacgtc gtagaacacc gcagatagtg tcccgcccaa tacacgcatg
     1561 ctgtgcgcat ggctgatctg ctccacttgt tgcttgtggg tgctggagag ttcgtccatg
     1621 tagcggtaga tctcctgcac atgcatctgc gtacgtcgct gctcctgcag gtaccgcacg
     1681 gtcttgagct tgctgcaggg gtgcatgatg cgcgagagga ccaggtgacg gaacatctca
     1741 tcgggaacgg ccccgaagcc gatctgctcg aagagccgcc ccagtacgag ttcgggaccc
     1801 agcaagatca catcaccgat actcttgaag gtggcatcga accggtcctg ttcgtcctgg
     1861 aggtgcaagt ccaaaccctg ctggcccgtg cgctgcgcta tgaaacgcga tgcccgctcc
     1921 ttcagatcat ggatgtcggc ctgttccttg gccgaaccga tcgaacacac cagtatgttc
     1981 ttcctgccac gcttttccag cacctgaaca ctcacgcttc ccgatctatt gcgcttctca
     2041 cggtaaaaca cacgcaaaga accgcacggg tcacccaaac ccgaccaacg cacccccgtc
     2101 tatcacggga tacaggccgt tgggcaggcc aaaacgtaaa atctgttcaa aacaggccag
     2161 tgggcaccat tcgtgggagt ccaacgtatg cgggcgaatg gtccatgcaa cctgaccttc
     2221 atcaggtgag cagtttgcga ggggtgataa ccttaccgtc catgccgcct gatccgcctc
     2281 ctcgcaacgg agggggcgcc atgaagttga tccgaaagcc cgcacccatg aacagaatcg
     2341 ttacaacggc cctcctcagt gcggtaacgg tcgtcgcgca agcccagacc gctatcaatt
     2401 tcaactgcaa cgactgcgcc ggtacgccac acgacctgtt cgccgagctt gatgccggca
     2461 aggtggtcgt cctggcctgg gtgatgccgt gcgccccgtg cgttgggccg agcctgacca
     2521 tgcgcaacat cgtgcagagc tatcaggctt ccgatccggg ccgggtgatc atgtacctct
     2581 gcgacgacta cgggaacacc agttgctccg cgctcaacgg ctggatgaac gccaacggtc
     2641 tttcgggcct caccagcttt tccaacccgg ccatcaacat gacggactac ggtgggcccg
     2701 atatgcccat gttcgctgtg gtcggtgggc ccgatcatgt cgtcttcttc agggaggacg
     2761 gcaccgtgga cgtgcccgcc gtccaggatg cgatcgcagc cgccctctcg gccacggcca
     2821 tcaccgagga gagcaacctc accggcgcac cttccattca tcccgtgccg gccacggatc
     2881 gggcatggat cgacatcccg ctcgcgcatg ctgcccgggt caccgtcgat ctgtacgacc
     2941 tttccggtag cctggtcaga agcgtccaca atggtccgct gctgcccggt gatcatcgta
     3001 tcggactgga cacgcgtgaa ataccggccg gaatgtatgt gatacgtgtc ctggcaggtg
     3061 aggagcgtgt tgccctcaac ctggtcatcg cccattgatc accgccatgc atcccatagg
     3121 acttggaatc tgtccgcatg caagaccatc cgaacaaccc atgaggaaca aaggtgcgcg
     3181 ttggttgctg atggccttta tgttatcggc cgggcctttc acgcaccgtt cactggcgca
     3241 atgctgcgcg ggcggaagcg gttcctgcat ggtaggaggg gcggcgcagg gggtgttggg
     3301 agcccaccag atggaactca acaccaactt ccagtacatc cattccaatt cgttctacag
     3361 gggcagtgag cgtgcgagtg aacacacctt cgacagcttc gagagcaagc tccagtactt
     3421 caaggtcgcc tatggcgcat cgaagaacct gaccgtgtcg ctggagtaca gccactacct
     3481 gctgaagaag gagacgggcc tgaacggcga ccccgccacc acgtacacct gcaaaggcat
     3541 gggtgacctg atcatcttcc cgcgttacaa catgttccac aaggagaccc gcagtagcag
     3601 cgatgaggtg accatcgggc tgggctacaa ggtcccgctc ggagcgtaca atgattccat
     3661 cggtaacgtg gagccgttct cgggacagac cttttacgtg accaaaccca cggcggtaca
     3721 gctctcatcg ggtgcccagg acctgatcgt ccacaccttc taccatcatg ggttcctgcg
     3781 gtccggcctc aacttcttcg ccagcgcctt gtacatccgc aagggctgga atcccaacgg
     3841 cgagaagatg ggcgacttcg ccagcctggc cgtgtacggc agccgcacct tcagcaaacg
     3901 ttatggcatc acattgcagg tccgatatga gtacgcggac cggatgcgga tcaacaataa
     3961 tatactcatg ttcggaaaac cttccaatta cttccccgag gccaccggct attgcaaatg
     4021 gttcttcacc ccgcagg
//
LOCUS       DAOQOC010000116         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10140_length_4036_cov_3.659633, whole genome shotgun sequence.
ACCESSION   DAOQOC010000116 DAOQOC010000000
VERSION     DAOQOC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10140_length_4036_cov_3.659633"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PL106_02875"
     CDS             <1..253
                     /locus_tag="PL106_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32041.1"
                     /translation="PLGELIELMGTYSTDDPAVSGVKSDLDALAGLFAALPTPAASTV
                     TQENGVAVIGGGAAPAAITDDQLKAITEKTATIRNGYIN"
     gene            259..696
                     /locus_tag="PL106_02880"
     CDS             259..696
                     /locus_tag="PL106_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32042.1"
                     /translation="MKHLHLLALIAAISALPTAATAQCRAFVKNKCAANMLPYKFNET
                     FNAAQLGPGEEAEVDLTFFSGQDYRLMVCAHPIIGEVNWKLMDGTGQTLFESLASAPK
                     PSFEFKVATTQKLKVHIDVPEDKSSATEMLNYGCVGILVGFKE"
     gene            complement(865..2490)
                     /locus_tag="PL106_02885"
     CDS             complement(865..2490)
                     /locus_tag="PL106_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32043.1"
                     /translation="MRRTCLVVTTLLSAFMATAQQGWLPLSTDIDGPIGGRIRSIKAD
                     PALHSGIRPYLREDLKLIPGADSLVPKAWRPWLDSLSKPRHGVYGTPFAEAMIGGSFG
                     EKDPIKFRTGLGGQLDWSAGKRWSLHVDGMVWAETLPNYLDSVVRSIHVTPGEGYAYG
                     DAGAGSVRTHHDWSAWVDHKAGQYFHITLGRGKNFFGEGLRSLFLSDNAYSYPYLRIT
                     TTVWKVRYVNLFAAMSDIRGTNGQWNSFDLKWTSMHYLSWNALSRLNISLFEAIIWQD
                     NDPAYRRGFDINYLNPVIFYRPAEFGLGSPDNALLGFAVNTRVGRRTLLYGQLMFDEF
                     LLREVRGGRGWFANKQGVQFGAVGHDAFNRKGLTLRAEFNYVRPFMYTHSDTRQNYAH
                     FNQPLAHPYGSNFWEALVQGEWRKDHWLVREVFSFAVLGQDTSTANDQSYGNNMFLPE
                     SARPVRDSDGRLENYGYYLGGPSMVHLYHNELRAGWIIEPRSGWMLEAAWTLRVRVPE
                     RGDDLMTNYLRLGISVNLRDRHVFQDARYVLAK"
     gene            complement(2487..3602)
                     /locus_tag="PL106_02890"
     CDS             complement(2487..3602)
                     /locus_tag="PL106_02890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNA32044.1"
                     /translation="MGPHRYILLLGFITSFVVTLFTMPSLIKVARMKHLVDEPSEERK
                     IHSRSVPTIGGIIIFAAILFSYSLWFPQASVLGFSIEDFKVLYQDMGAAYKDFKFTIA
                     VMVLLFFIGVKDDIIGFSPVKKLVGHMIVAYILVVMGGVRIRDMHGILGVYELPEEIS
                     IAFSCFTYVVLVNAFNLIDGVDGLAAGIGLIAASAYGLWLYWAGDVALALLAFTLAGA
                     LVGFLVFNYHPARIFMGDSGSLIIGAIISILAMRVIDHDVSKLPRWLAAVPTPLFAMS
                     AIAYPLVDTLRIFSIRLAKGRSPLAADRNHIHHRLMDLGLGHRSVTLILYCYAVAMIA
                     LSLITRDILPNKGLLVLGVTAFVLAVLPFTLTSRKAP"
     gene            complement(3609..>4036)
                     /locus_tag="PL106_02895"
     CDS             complement(3609..>4036)
                     /locus_tag="PL106_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32045.1"
                     /translation="PFAYTSSFTRGSYSQYNEPLASPLGAGYNEAVAILDYGINQTWW
                     FRVQGSLATFTVDTTATTVSGGNIFDPRDAMGADSLAVKRTRAWLDVSFAWRINHNQN
                     TSLSIGLSTRDVNPAPDTQNCHYLYVCLRTGLFNRYYDH"
BASE COUNT          797 a         1262 c         1257 g          720 t
ORIGIN      
        1 cccattgggc gagttgatcg aactgatggg cacgtacagc accgatgatc ccgcggtgag
       61 cggtgtgaag agcgacctgg acgcactcgc gggtctcttc gccgcactgc ccacgccggc
      121 cgccagcacc gttacccagg agaacggcgt tgccgtgatc ggcggcgggg ccgccccggc
      181 cgccatcacc gatgaccagt tgaaggccat caccgagaag accgccacca tccgcaacgg
      241 ctatatcaac tgatcccgat gaagcacctc catctgctcg ccctcatcgc cgcgatctcc
      301 gcattgccca cggccgccac cgcgcaatgc cgtgcgttcg tgaagaacaa gtgcgccgcg
      361 aacatgttgc cgtacaagtt caacgagacc ttcaatgccg cccaactcgg ccccggcgag
      421 gaggcggagg tggacctgac cttcttcagc ggccaggact acaggctcat ggtgtgcgcc
      481 cacccgatca tcggcgaagt gaactggaaa cttatggatg gtaccggtca gacgcttttt
      541 gaaagcctcg ccagcgcccc caaacccagt ttcgagttca aggtggccac cacgcagaag
      601 ctcaaagtgc atattgacgt gccagaggac aagtcttccg cgaccgagat gctcaactac
      661 ggatgcgtcg gtatcctggt cggcttcaag gagtagccct ccatcgatat tcaaccggac
      721 aacgcggtgg acccgtcgaa gcggtcgaac aagctcaccg tgtcccgccc ttggtatggc
      781 gccggacgat cggagcggag gtccaatggt ccccggcgca cccggtgatc cctgtcgcca
      841 ggggtcaagt tccggagttg ggccctactt cgcaagcacg taccgtgcgt cctggaacac
      901 gtgccggtcc cgcaggttca ccgagatccc caagcgcagg tagttcgtca tcaggtcatc
      961 gccccgctcc ggcacacgca cacgcaaggt ccaggcggcc tccagcatcc atccgctgcg
     1021 cggctcgatg atccagccgg cccgtagttc gttatggtac agatgtacca tgctgggtcc
     1081 tcccaggtag tagccgtagt tctccaggcg cccatcgcta tcgcgcacag gccgggcact
     1141 ctcgggcagg aacatgttgt tgccgtagct ctggtcgttc gccgtactgg tgtcctggcc
     1201 cagcacggcg aaactgaaca cctcgcgtac gagccagtgg tccttccgcc actcgccttg
     1261 gacgagagcc tcccagaaat tgctcccgta cgggtgcgcc aacggctggt tgaaatgcgc
     1321 gtagttctgc cgcgtgtcgc tgtgcgtgta catgaagggg cgcacgtaat tgaactcggc
     1381 acgaagggtg aggcccttcc ggttgaacgc atcgtggccc accgcaccga actgaacgcc
     1441 ctgcttgttg gcgaaccatc cacggccccc gcgcacttcg cgcaacagga actcgtcgaa
     1501 catgagctgg ccgtacagga gcgtcctgcg gcccacccgg gtattcaccg cgaaaccgag
     1561 caacgcgttg tccggcgagc ccagtccgaa ttcggcaggc cggtagaaga tcaccgggtt
     1621 caggtaattg atgtcgaagc cccgacggta cgccgggtcg ttatcctgcc agatgatggc
     1681 ttcgaaaagc gagatgttca gtcggctcaa cgcgttccag ctgaggtagt gcatgctggt
     1741 ccacttcaga tcgaagctgt tccactgccc gttggtgccg cgtatgtcac tcatggcggc
     1801 gaagaggttc acgtaccgca ccttccacac ggtggtcgtg atcctcaagt agggatagct
     1861 gtacgcattg tcgctgagga acaaggagcg caacccctcg ccgaagaaat tcttgccgcg
     1921 ccccagggtg atgtggaagt actgtccagc cttgtggtcc acccaagcgc tccaatcatg
     1981 atgggtgcgc acggagcccg cccctgcgtc cccgtacgca tagccctccc cgggcgtcac
     2041 atggatggac cgcacgacgc tgtccaaata attggggagt gtttcagccc agaccatgcc
     2101 gtccacgtgc aggctccaac gcttcccggc actccagtcc agttggccac ccagcccggt
     2161 ccggaacttg atcggatcct tctcgccgaa tgaaccaccg atcatcgctt cggcgaaggg
     2221 cgtaccgtac acaccatgcc ggggtttgga gaggctgtcc agccagggcc gccacgcctt
     2281 ggggaccaat gagtccgcac cggggatcag cttcaggtcc tcccgcagat aggggcgaat
     2341 gccactgtgg agtgcgggat cggccttgat ggaccggata cggcctccga tggggccgtc
     2401 gatgtccgtg ctcaaaggca accatccctg ttgggcggta gccatgaacg cggaaagcaa
     2461 ggtggtcacc accagacagg tccttctcat ggggccttgc gggaagtgag cgtgaaggga
     2521 agcaccgcca gtacgaatgc ggtgacaccg agaacgagga gccccttgtt cggcaggatg
     2581 tcgcgtgtga tcaggctgag cgcgatcatg gccaccgcgt agcaataaag gatgagggtg
     2641 acgctccggt gcccgagacc gagatccatc aggcggtggt ggatgtggtt gcggtcggcc
     2701 gccagtggac tgcggccctt ggcaaggcgg atgctgaaga tgcgcagggt gtccacaagc
     2761 ggataggcga tcgcgctcat ggcgaagagc ggcgtgggca cggcggccag ccagcgcggc
     2821 agcttgctga cgtcgtgatc gatcacccgc atggccagga tgctgatgat ggccccgatg
     2881 atgagcgacc cgctgtcacc catgaaaata cgagcgggat ggtagttgaa gaccaggaag
     2941 cccaccaggg caccggccag ggtgaaggcc aagagggcca gcgcaacatc gcccgcccag
     3001 tacagccaaa ggccataggc gctggcagcg atcaggccga tgccggcggc caggccgtcc
     3061 accccgtcga tcaggttgaa tgcgttcacc agcaccacgt acgtgaagca tgagaacgcg
     3121 atggagatct cctccggcag ctcgtacacg ccgaggatgc cgtgcatgtc ccggatccgc
     3181 accccgccca tcaccaccag gatgtacgcc acgatcatgt ggcccaccag cttcttcacc
     3241 gggctgaagc cgatgatgtc gtccttcacg ccgatgaaga acaagagcac catcaccgca
     3301 atggtgaact tgaagtcctt gtacgccgca cccatgtcct ggtacaggac cttgaaatcc
     3361 tcgatgctga agcccaacac cgaggcctgc gggaaccaca ggctgtagct gaagaggatc
     3421 gctgcgaaga tgatgatgcc gccgatggtg ggcacgctac ggctgtggat cttgcgttcc
     3481 tcgctgggct catcgaccag gtgcttcatg cgcgctacct tgatcaggct gggcatggtg
     3541 aagagggtca ccacgaagga ggtgatgaac ccgaggagca ggatgtaccg gtgcggtccc
     3601 aatcggtgtc agtgatcgta gtagcggttg aacaaccccg tgcgcaagca gacgtagagg
     3661 tagtggcagt tctgcgtgtc cggagcgggg ttcacatcac gtgtactgag gccgatgctc
     3721 aggctcgtgt tctggttgtg gttgatgcgc cacgcgaaac tcacgtccag ccatgcccgg
     3781 gtgcgcttca ccgccaggct gtcggccccc atggcatcgc gcggatcgaa gatgttcccc
     3841 ccgctcaccg tggtggccgt ggtgtccacg gtgaaggtgg ccaggctgcc ctgcacgcgg
     3901 aaccaccacg tctggttgat gccgtagtcg aggatggcga cggcttcgtt gtatccggca
     3961 cccaaggggc tcgccagcgg ttcgttgtac tggctgtagc tgccgcgggt gaagctgctg
     4021 gtgtacgcga acgggg
//
LOCUS       DAOQOC010000117         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10229_length_4014_cov_4.315736, whole genome shotgun sequence.
ACCESSION   DAOQOC010000117 DAOQOC010000000
VERSION     DAOQOC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10229_length_4014_cov_4.315736"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1512
                     /locus_tag="PL106_02900"
     CDS             175..1512
                     /locus_tag="PL106_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNA32046.1"
                     /translation="MNPTALCWKKTETYQKRSTHQEHVDPTIMLLSGRDRRSTDGHVP
                     SDGIRSSARGIGSFTLTRRYRTTDFRNQPKVRGNGPEKQPNHNSTMRTGLTLFTAGLL
                     AGAVSAQGVITRMNAGTSEFYYDGNIQAVLDEAFEEAGTDTILLGGGTYNMTSDILIS
                     SPVVMIGTGIHPDSSLAYNNNGRTEIAGGQFMEWFLENDASGSEFHGIAFTNEVDVQF
                     GTGPIESTDVNDVKFFRCEFQGLGLGNGQFGSMANDTYIHECVFRGGFSNGEAQGSII
                     RNSAIKQVYDILNGNDCLFENCIFFSSIGGGSGVQYENNVFLLNQGSNYNINQQSTYL
                     NNLFVGTGAGFGVTFGVGVVANGNEFDYPLNGANGAFPSATVTDWNNFDFYADYHLAT
                     AWSGFDFGLFGGGDPWKEGSVPFNPHWRELTTPTGTENGTLQGVTIKASAQQD"
     gene            1516..2769
                     /locus_tag="PL106_02905"
     CDS             1516..2769
                     /locus_tag="PL106_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32047.1"
                     /translation="MRYLLTALVLLASGWGSAQNTIVGYEHWLDEQDDDGERTYVPAN
                     GTSINLSGIDIPVDQLSLGLHRVHFRLRDANAAWSSVLRRSFYIASGATVALTSGEYW
                     FDQDDDTRIPFDFSDGQNIDITIDPSVSGLELGMHRVHFRIRDDQGQWSAVLTRTVHI
                     ESGALVKLTLLRYWSDDSQGFPDDMTTMAIDPEVEVWDVVDEIEFCTWETTGNTNVYF
                     QLKDNQDQWSSVIKRTFDIDLVATGPGAVGTITGPVVVGTETTVTYSVPVVTGAATYT
                     WTYPSGWTVVGNANGNSITFQTPADFLDGVVTVTASNACGESGPSSLNVQLDDTSLDP
                     TSASGGLDLYPNPSTGQFILTGNSPAVIDRLTIHNATGQLVQDFRPAQTDRLTLDLSN
                     EANGLYTVRIIQGDQHTKLQVMVQH"
     gene            2866..3936
                     /locus_tag="PL106_02910"
     CDS             2866..3936
                     /locus_tag="PL106_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32048.1"
                     /translation="MKSFWTLATCHLLITATVNAQGVITRIGSDGTHFYYNGQLDSAV
                     AHANLTTTTDTILLGGGTYSVPTTDLIIEQPIILVGTGPDIDSTAAYGGRTVIDGNDI
                     FLRDGADSSEFHGLTITSNVFIGEFELNTTDVDHLLFRRCDLQSLRLNALMSPGATMA
                     NDVTLDNCVVRTNNLNLNGAANVILTNSVLKEFNSAANGTLVEHCLFLNWNLGNGPAG
                     VTFRNNIFANDLCCSASVNGASTYINNLFVGNGDDFIVNYGMAVSHDDIDPVGTSLGS
                     VFVNVSSYNQFTYGGDADYHLLSDWQGLGDDGTDPGPHGGNGWKEGGLPFSPHWRYLN
                     MPASTVNGVLPNVHIKAGAQEN"
BASE COUNT          934 a         1198 c         1079 g          803 t
ORIGIN      
        1 ccgtgagccg ccgatacaag caggattttt tcgccctgtt cgaccgggtg aagtaggacc
       61 caccacattc ctcgccgaaa ccgccgcttt ccattcgcca cgcggacaca ccgctcatgc
      121 ccggcatttt cgaccgcacg gccagtatcc gaaaccccga ctaccaggat gggaatgaac
      181 cctactgcac tctgttggaa gaaaaccgaa acctaccaga agagatccac acatcaggaa
      241 catgtcgatc caactatcat gctactttcc gggcgtgacc ggaggagcac cgatggccat
      301 gtcccttctg atggcattcg ctcatcagcg cgcgggatag gctcattcac gctcactcgg
      361 cgatacagaa cgactgactt tcgcaaccaa cccaaggtcc ggggcaacgg gccggaaaaa
      421 caacccaacc acaacagtac catgagaaca ggacttacac tttttactgc cgggcttttg
      481 gccggggcgg ttagcgcgca gggagtgatc acgcggatga atgcggggac ctcggaattc
      541 tactacgacg gcaacattca agccgtactt gatgaagcct ttgaagaagc tggaaccgac
      601 acgatcctgc ttggtggggg cacctacaac atgacctccg acatcctgat cagttcaccc
      661 gtggtcatga tcggcacggg aatccaccct gatagctctc ttgcatacaa caacaacggc
      721 cgtaccgaga tcgctggcgg tcagttcatg gagtggttcc tcgagaacga tgcttcagga
      781 tcagaattcc acggtattgc tttcacaaat gaagtggatg tccaattcgg tacagggccg
      841 atagaatcaa cggatgtcaa tgatgtgaag ttcttccgat gtgaatttca aggactcggt
      901 ctcggaaacg ggcaattcgg gtcgatggcc aacgacacgt acatccacga gtgcgttttc
      961 agaggcggat tcagtaatgg cgaagcgcag ggttcaatca tccgtaactc agcgatcaaa
     1021 caggtatacg acattctgaa cggcaatgac tgcctcttcg agaactgcat ctttttctcc
     1081 agcatcggcg gcggctcagg ggttcaatac gagaacaatg tcttcctgtt gaaccagggg
     1141 tcgaactaca acatcaatca acagagcacc tacctgaaca atctattcgt gggtacgggt
     1201 gctggcttcg gcgtgacctt cggtgtgggc gtggtagcca atggcaacga attcgactat
     1261 ccacttaatg gagccaatgg cgcgttcccg agcgcgaccg ttaccgattg gaacaatttc
     1321 gacttctacg cggactatca cctcgcaacc gcatggagtg ggtttgattt cggccttttc
     1381 ggtggaggcg acccctggaa agagggttcg gttccattca atccgcattg gcgagagctg
     1441 accactccga ccggtaccga gaacggcaca ctccaaggcg tgacgatcaa agcctcggcc
     1501 caacaagact gagccatgcg gtacttgttg accgcgctcg tgctcctggc ctcgggctgg
     1561 gggagcgcac agaacaccat cgtcggctat gagcactggc tcgacgaaca ggacgacgac
     1621 ggcgaacgga cctacgttcc ggccaacggg accagtatca acctctccgg gatcgacata
     1681 cccgtggacc aattgtcgct gggcctgcac cgcgtacact tccgcctacg cgatgccaat
     1741 gcagcatgga gcagcgttct ccgccgcagc ttctacatcg cttcgggcgc aaccgtggcc
     1801 ttgacctccg gggagtactg gttcgaccag gatgatgata cgcgcatccc tttcgatttc
     1861 tctgatgggc agaacatcga catcaccatc gaccccagcg tgagcgggct tgaactgggt
     1921 atgcatcgcg tccatttcag gatccgggat gaccaaggcc aatggagcgc tgttctcaca
     1981 aggaccgtac acatcgagtc cggtgcgctg gtgaaactca ccctgctccg ctactggagc
     2041 gatgattcgc agggctttcc ggatgacatg accaccatgg ccatcgaccc cgaagtggag
     2101 gtctgggatg tggtggacga gatcgaattc tgcacctggg aaaccaccgg taacaccaat
     2161 gtctacttcc agttgaagga caaccaggac caatggagct cggtgatcaa acgcaccttc
     2221 gacatcgacc tggtggcgac tggcccgggt gctgtgggca cgatcaccgg cccagtggtc
     2281 gttggaacgg aaaccaccgt gacgtactcg gtacccgttg tgaccggcgc ggccacctac
     2341 acgtggacct acccgtccgg atggaccgta gtgggcaacg ccaacggcaa ttcgatcacg
     2401 ttccaaacac ccgcagactt ccttgatggt gtagtgacgg tgacagcgag caacgcttgc
     2461 ggggaaagtg gcccctcctc gttgaacgtg caactcgacg acaccagtct ggacccgacc
     2521 agcgcttcag gtggtttgga tctctatccg aacccgagta ctggccagtt cattcttacg
     2581 gggaactcgc ccgctgtgat cgaccgcctc accatccaca acgccaccgg ccagttggtg
     2641 caggacttcc gccccgcgca aaccgaccgc ctcacccttg acttgagcaa cgaggccaac
     2701 ggcctgtaca ccgtgcgcat catccaaggc gaccaacaca ccaagctgca ggtgatggtg
     2761 cagcattgaa gaaccactga acatccactg acctcagggt cccgcccgct tggaattacc
     2821 gggtgggcgg agaccctggt aaatttaaac acgatcatct gacacatgaa gagcttctgg
     2881 accctggcca cctgccacct gctcataaca gcaaccgtga atgcacaagg cgtgatcaca
     2941 cgcataggaa gtgatggcac ccacttctac tacaacggcc aactggacag cgcggtcgct
     3001 catgccaact tgaccaccac gacggatacg atcctactcg gaggtggtac atactcggtt
     3061 ccaaccacgg atctcatcat cgagcaaccc atcatactcg tgggcacagg gcccgacatt
     3121 gatagcacgg cagcttatgg aggccgcacg gtgatcgacg gaaatgacat cttcctgcgt
     3181 gatggtgctg atagctccga gttccatggg cttaccatca ccagcaacgt cttcatcggc
     3241 gagttcgaac tgaacactac ggatgtcgac cacctcctct tccgcaggtg cgacctgcaa
     3301 tcgctccgat tgaacgccct gatgagccct ggtgcgacca tggcgaacga cgtcacgttg
     3361 gacaattgtg tggtgcgcac gaacaacctg aacttgaacg gagccgcaaa cgtgatcctg
     3421 accaactccg ttctgaagga gttcaactcc gccgccaacg gcacccttgt cgagcattgc
     3481 ctgttcctca actggaacct gggaaacggc ccggccgggg tcaccttccg gaacaacata
     3541 ttcgcgaacg acctgtgctg ttcggcaagt gtcaacggcg cgagcaccta catcaacaat
     3601 ctcttcgtgg gcaatggcga cgatttcatt gtgaactacg gcatggcggt ctcccacgac
     3661 gatatcgatc ccgttggcac ttcgttgggc agtgtgttcg tgaacgtgag ttcatacaac
     3721 caattcacct atggtggtga cgcggattac catttgctga gcgactggca aggattgggc
     3781 gacgatggta ccgatcccgg tccacacggt ggcaacgggt ggaaggaagg cggacttccc
     3841 ttttcgcctc attggagata cctgaacatg cctgcgagca ccgtgaacgg tgtacttccg
     3901 aacgtccaca tcaaggccgg cgcccaagag aattgaacca tgcgctatac gttctcttcc
     3961 attctgctcg tagccgtctg tgcggctacg gcacagaccc agatagcggg catt
//
LOCUS       DAOQOC010000118         4012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10242_length_4012_cov_4.352287, whole genome shotgun sequence.
ACCESSION   DAOQOC010000118 DAOQOC010000000
VERSION     DAOQOC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10242_length_4012_cov_4.352287"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PL106_02915"
     CDS             <1..1125
                     /locus_tag="PL106_02915"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA32049.1"
                     /translation="FLGSTTGLGIAHFDVNGSNGDVPHGHPNHWLLPLDPVVHVSPGD
                     ALNIAVTGMPGNVGNLVVCYVDLNNDLDFDDTQETSSYVQGSSQDQVMTFTLGVPAGA
                     AAGGHWVRIASYGQGFEFVNGCTDPVAGQVVDTYIIVNDPSGPCIPGDISWTTDGHFI
                     DGVQLGDIQNTGSGARFGAAYTDHPQSTTDLIVTHDYEVTVTSGMQDISTYRAWIDYN
                     NDLDFDDPGELLGSATSDAAYQSLSIPFSVPEGTPLGTTYLRVRCDFGAEPSACEDGQ
                     WGETEDYRVEMVTNTGTDLEAQEPFTVTAGEAGWLVRVGRGLEKSQYSLVDGAGREVQ
                     SGQIAGSTLLLLAGSLSNGSYALRLTAMDRVWSKLLFVVQ"
     gene            1314..2384
                     /locus_tag="PL106_02920"
     CDS             1314..2384
                     /locus_tag="PL106_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32050.1"
                     /translation="MASDLLFAHPRRRDPFYQVGDFILRTELSYQDAAARFREFRRSE
                     GDGFPWGDMLREVLDINTVTVTKRIQAIHGNGTRGTVTLKNFFKVSNNDLIDLHDHSG
                     QDVERLSIEDLNVAAQWYERRYGGGQGALYYLEATAIVATLPFGGAARQASSRITTML
                     ARRAAAATARYLLRRGCRRLVRFLLARATRSVAAFLLKFTRDFVTSYVRACRLSDLQN
                     EIRVSAGRHPAPMERDRLLREACAGAVAENLIGEVLGGLGEALDEVDLGAAIGEQVKQ
                     AIGNHLSRRLLAVAAVPFQEIIRSSIAYTHDPSSGKSFSEGLTAHMMNAIQERFTERM
                     VADEFRSAVAAIGENPGLVAEN"
     gene            3368..3874
                     /locus_tag="PL106_02925"
     CDS             3368..3874
                     /locus_tag="PL106_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32051.1"
                     /translation="MTLSLEGREDRSFCSGSAQVMLHSLFITGESERYVHSVIFSPGS
                     RDSVTAGRYQATLWKKPKGQRSQVMVTVAERMKPEAPSYSMAEGYVDLEIADGERFKG
                     TFNGTAKNTKNQQEQLRMTGAFDVKFDASKNVKDFDALDSIVKAESHGKKDLKETIRR
                     EAKENKLE"
BASE COUNT          894 a         1138 c         1180 g          800 t
ORIGIN      
        1 ttcctgggct ccacaacggg ccttggcatc gcgcacttcg atgtgaacgg ctcgaatggc
       61 gatgtgcccc atggccaccc gaaccactgg ctcttgccgc tcgaccctgt ggtacatgta
      121 tcgcccgggg atgcgctcaa catcgccgtt accggtatgc cgggaaatgt gggcaatctc
      181 gttgtctgct acgtggacct gaacaatgac ctggacttcg acgacaccca ggagaccagt
      241 tcctatgtgc aaggctcgtc gcaggatcag gtgatgacct tcacgctggg cgttccggcc
      301 ggtgcggcag ccggcgggca ctgggtgcgc atcgcgagct acggccaggg ctttgaattc
      361 gtgaacggat gcaccgaccc ggtcgcagga caggtcgtgg acacttacat catcgtcaat
      421 gatccttcgg gtccttgcat ccccggcgat atcagttgga ccacggatgg tcatttcatc
      481 gatggcgtgc aattgggcga catccagaac accggctccg gtgcacgctt cggtgcggcc
      541 tacaccgacc acccgcaaag caccacggac ctcattgtga cgcacgacta tgaagtcacc
      601 gttacctctg gcatgcagga catttcgacc taccgcgcct ggatcgacta caacaacgat
      661 ctggatttcg atgatcccgg tgaactgttg ggttcggcca ccagcgatgc ggcctaccaa
      721 tcactttcga tcccattctc cgtcccggaa ggcacgccgc tagggacaac gtaccttcgt
      781 gtgcgctgcg acttcggtgc cgaacccagt gcttgcgagg atgggcagtg gggggagacg
      841 gaggattaca gggtggagat ggtgacgaat acgggaacgg atctggaggc acaagaaccg
      901 ttcacagtca ctgctggcga ggcaggatgg ctggtgaggg tggggcgggg gctggagaag
      961 agccaatact ccctggtgga tggggctgga cgggaagtgc agagcggtca gatagccggt
     1021 agcacactat tgctgttggc gggtagcctt tcgaatggaa gctatgcgtt gcgcctgacg
     1081 gcgatggacc gcgtatggag caagctgctc tttgttgtgc aatgaacgct ccgtgcctat
     1141 gaccagcaac cgcttcttac acgggaattg tctgaccagc ctttcacact gagaaccatt
     1201 caatgccaca gcgacggttc gtcatacacg gaggtcacct gcatgaactg gtatatgaca
     1261 cgtccggctt tttggggacc gctcttggca tacgagagga acatgaggtt cgcgtggcga
     1321 gtgacctgct cttcgcgcac ccgaggcgac gcgatccgtt ctaccaagta ggggatttca
     1381 ttctccgcac cgaattgtca tatcaggatg cggcggcccg attcagggaa ttccgccgct
     1441 ccgaagggga tggattccct tggggcgaca tgctcaggga ggtgctggac atcaacacgg
     1501 tcactgtcac caagcggatc caagccattc atggaaatgg cacccgcggc acggtgacgc
     1561 taaagaactt tttcaaggtc agcaacaacg acctgatcga tctgcatgat cattcaggtc
     1621 aggatgtcga gcggctgtcg atcgaagatc tgaacgtcgc ggcccaatgg tacgagcgtc
     1681 gatatggcgg tggccaaggg gcgctatact acttggaagc caccgccatt gtcgccacgc
     1741 ttccatttgg aggtgccgcg cgccaagctt cgtcaaggat caccaccatg ctcgcgcgca
     1801 gggccgcggc agcgaccgct agatacttgt tgcgacgagg atgcaggcga ctggttcgct
     1861 tccttctggc gagggccaca aggagtgtag cggcgttctt gctgaagttc acaagggatt
     1921 tcgtcaccag ctatgtcagg gcatgcagac tttcagacct gcagaatgag atccgtgttt
     1981 ccgctggtcg gcatccggca ccgatggaac gtgaccggct attgcgggaa gcctgcgcag
     2041 gcgctgtagc cgagaatctt atcggtgagg tcctgggcgg tctgggagag gctttggacg
     2101 aggtagacct gggagccgcg atcggtgaac aggtcaaaca ggctatcggc aaccatttgt
     2161 cgcggcgttt gcttgcagta gcggctgtgc ccttccagga gatcattcga tcgtcgatcg
     2221 cctacacgca tgacccctcc agcggcaagt cgttttcaga ggggctcacg gcacacatga
     2281 tgaatgctat acaggaacgc tttactgaac ggatggtggc cgatgaattc aggtcagctg
     2341 tggccgctat tggagaaaac cccggcctgg tggccgagaa ttgagctaac attcatcagc
     2401 cgaacatcct caattctcgc gcgagcatcc acagtcgtgc ggcagtccga tgatcaccaa
     2461 ttcacccttc cactgaacaa ttaagtccct gaaacatgtc tgacatctcc gattccctac
     2521 gcgcggcagc actgcattat cccattaacc aaactgttgg cgttggcaat tgctgggcgt
     2581 tcgtgaatga agcgctggtg gccgcaggtg gaagaagccg ttatggccgg gcaggagccg
     2641 ctctctatca atggggagag gtggtgagac aaaggacttc agtgcgccct gggcacgtaa
     2701 tccagttcag cgcgatcagc ttcttccgtt tcgcaccccc gccgggcacg ccaccaacag
     2761 ttcccgcacc accccgcagt accgggccat gccatagcgc gatcgtaatt gacgtctaca
     2821 atcagggaaa gcaaatgctc atccggcatc agaatttcgg agttgcgacc gtcagacaaa
     2881 tgtactggta ctttgaacca ggtatctaca cggaagatgg aagtcggact gtcaccatag
     2941 cggaggttga caacacggta acattctata gacccaatac gacttcctga cgtcatgttc
     3001 tccatgccgg cattggaagg aagaaaaccg atatcgccat cgatcgcacg cgcgtatcac
     3061 gcctgccaca tgaaacactg tccaattctc gcctgagtgt ctgcgctcgt gcatttcaat
     3121 ccagcgccgt ggcaccccca cgctgcacca ccatggtcac ctcaatttcc cgctccggat
     3181 gcactcaaca gcagggatca acaacatgaa aaaggccata aagcacattc tggcctggat
     3241 gctcacaacg gcagcgtgct attcgtgctc atcaggcgcg cgtaagcagg acccatcggt
     3301 cgcggcagaa cctcaacagc aggcagggcc gcgagccgac gcccatgctc cctgtgacca
     3361 cccgaacatg acgctttctc tcgaaggacg ggaagatcgc tcgttctgca gcggctcagc
     3421 gcaggtgatg cttcacagcc tgttcatcac cggtgaaagt gagcgctacg tccacagcgt
     3481 gatattctca cccggaagcc gtgattcggt caccgctgga aggtatcaag ccacgctttg
     3541 gaagaagccg aaaggccaac gctcccaggt gatggtgacc gtcgctgaac gcatgaaacc
     3601 ggaggccccg agctacagca tggctgaggg atatgtcgat ctggagattg ccgatggcga
     3661 acggttcaaa ggaacattca acgggacagc gaagaacacg aagaaccagc aggagcagtt
     3721 gcggatgaca ggagctttcg atgtcaagtt cgacgcgtcc aagaacgtca aggactttga
     3781 cgcgctcgat tcgatcgtga aggctgaaag ccatggcaaa aaggacctga aggagaccat
     3841 ccgtcgcgag gccaaggaga acaaactgga gtaacgcgag catcttgaac gcgtgaacta
     3901 cgaagccccg gtaatgagcc ggggcttcgc actgtcagta ggagaggagc ctacgccgtc
     3961 ggctcgctcg ccggcggcag ttccttggcg tcgtccgcct tgccaagctt ct
//
LOCUS       DAOQOC010000119         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10253_length_4009_cov_3.210420, whole genome shotgun sequence.
ACCESSION   DAOQOC010000119 DAOQOC010000000
VERSION     DAOQOC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10253_length_4009_cov_3.210420"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PL106_02930"
     CDS             complement(<1..1038)
                     /locus_tag="PL106_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA32052.1"
                     /translation="MSGENTVHRKTVHLEALDHKGQRRIALRFPYDAWLITIAKQLGA
                     QWSKSHKCWHVENGSPSMKAIFAAYKGLAWVNAEALFKKEGNAQQPVPATSIQPKQPS
                     RPVETPMGPAQAEALQRMRQKLEIARYSPRTIEAYLNATKHFFLHFPGKHPGDIRTED
                     IEAYQHHMATERKVSNSHLNQVVNAVRYYYMNVVGDAQRVTFIERPRQEKKLPLVLSE
                     TEVAALLRSVDNLKHRCILMLIYSAGLRLSELVGLDLTDLVTDRHQLLVRGGKGNKDR
                     ITLLSPKALETVQTYITAYKPVRHLFEGPNGAAYSPRSVQAIFHRAKERAGIAKPATV
                     HTLRHSFATHLL"
     gene            complement(1125..2426)
                     /gene="murF"
                     /locus_tag="PL106_02935"
     CDS             complement(1125..2426)
                     /gene="murF"
                     /locus_tag="PL106_02935"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNA32053.1"
                     /translation="MTDITRLHTAYLECSGANYDTRALGAGEMFFALKDKRDGNAFAA
                     DALAKGARYAVVDDPAVVRDERYLLVPDVLQALQDLGRHHRRTFDIPVIGVTGTNGKT
                     TTKELVHAVLAADRPTLATEGNLNNHIGVPLTLLKLKAEHRIAVIEMGASKRGDIAEL
                     CAIAEPTHGLITNIGKAHLEGFVSVEGVIATKTELYEWLRAHRGTGFVNGDDPLLMAK
                     SEGLARITYGNAEGLNVRGNAVASAEPCMELVFDDNQREHGHYHIATQLIGNYNAPNA
                     LAAVCIGHHFGVPGETIAGAITDYQPANSRSQFKDTGRNQLILDAYNANPTSMRAALE
                     NFAAMHSNRKKLAILGDMLELGAVSQAEHKGIIALTQQLGLEAVFVGSEFMRAASGSD
                     VPCHASVADALKALQAAPPTGCLILVKGSRGVKLEKVLVVL"
     gene            complement(2494..>4009)
                     /locus_tag="PL106_02940"
     CDS             complement(2494..>4009)
                     /locus_tag="PL106_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNA32054.1"
                     /translation="MDEVEVTNFYWLEYLYWLDRVYGADFPEVYKKALPDTLVWRSKL
                     AFTEPYTEYYLRHPAYRDYPVVGVNWLQANDYCAWRTDRVNEVILIREGLFEHYPNQI
                     NEDHFTTDSYLAGQYESGKKVDGVQDFNPNKDTRNIKMEDGVLLPRYRLPTEAEWEYA
                     SYGLVGNTVDERIVERRIYPWNGHWVRYDSRKKGGAFYGDFRGNFMRGRGDYMGVAGS
                     LNDNADVTAPVFSYWPNDYGLYNMAGNVSEWVMDVYRPLTGEDENDFRPFRGNVFKTK
                     VLNSDGGIKDKYDKVIYDIDGIKYWLEEFQKRMEGKFTDEEGKLVEDLMTKINQAQEF
                     ASTRKPDPAQQNVQDMVDMIKGQDLEICPKLLSGISDYQSDQPGEVQMRKVTVEENID
                     RRNYQESDNIDFKDGDLESSVYYLQGDEPENGGMYANSGEVAFQDYLTLISDHARVYK
                     GASWADRIYWANPGTRRYLDERQSTATIGFRCAMVRVGSPVGLGDDKRRSKLKK"
BASE COUNT          615 a         1165 c         1313 g          916 t
ORIGIN      
        1 gaggagatgc gtggcaaagc tgtggcgtaa ggtatgcacg gtggctggtt tcgcaatacc
       61 ggccctttcc ttggctcgat ggaagatggc ctgtacgctg cgtggggagt aggctgctcc
      121 gttcgggcct tcaaagaggt ggcgcaccgg tttgtatgcc gtgatatagg tctgtacggt
      181 ttccagggct ttagggctta gcaaggtgat gcggtccttg ttgcctttgc ctccccggac
      241 cagcagttgg tgccggtcgg ttaccaggtc tgtcagatcc aagcccacga gctcgctgag
      301 cctcaaaccg gcggagtaga tcaacatcag gatgcagcgg tgcttcaaat tgtccaccga
      361 gcgcaggagg gcagccacct cggtttcgct gagcaccaag ggcagtttct tttcctgccg
      421 cggccgctca atgaaggtta cccgttgggc atcccccacc acgttcatgt agtagtagcg
      481 cacggcgttc actacttggt tcaggtggct gttgctcacc ttgcgctccg tggccatgtg
      541 gtgctggtag gcttcgatgt cctccgtgcg gatgtcgcca gggtgcttgc cggggaagtg
      601 aaggaagaag tgcttggtgg cattgaggta cgcttcgatg gtgcgcgggc tgtagcgggc
      661 gatctccagt ttctggcgca tgcgctgcag tgcttcagcc tgtgccgggc ccatcggggt
      721 ttcaactggc cgtgaaggtt gcttgggctg tatgctggtt gccgggacgg gttgttgggc
      781 gtttccttct tttttgaaaa gtgcctcagc gttcacccag gcgagcccct tgtatgcggc
      841 gaagatggcc ttcatgctgg ggctgccgtt ctccacatgc cagcacttat ggcttttgct
      901 ccactgcgcg ccgagctgct tggcgatggt gatcagccac gcatcatacg ggaagcgcag
      961 ggcgatgcgg cgctggccct tgtggtccaa ggcttccaga tgcacggttt tccgatggac
     1021 ggtgttttcg ccggacatgg tgcgctgaag atagggagcg tacaggtcga aagccgcggg
     1081 ttcttctttg gggatccgct gctggtagaa gcggcggcta ctgctcacaa caccaccagc
     1141 accttctcca gcttcacacc cctggagcct ttcaccagga tcaggcagcc cgtgggcggt
     1201 gcggcttgca gggccttcaa tgcatcagcg acgctggcat gacacggcac gtcactgccg
     1261 ctggcggccc gcatgaactc gctgcccacg aacacggctt ccagtccgag ttgttgcgtg
     1321 agggcgatga tgcccttgtg ctccgcctgg ctcaccgcac ccagctccag catgtcgccc
     1381 aggatggcca gtttcttcct gttactgtgc atcgcggcga aattttccaa agcggcccgc
     1441 atgctggtgg ggttggcgtt gtaggcgtcc aggatcagct ggttgcggcc ggtgtccttg
     1501 aattggctgc ggctgttggc gggctggtag tcggtgatgg cgcctgcgat cgtctcgccg
     1561 ggcacgccga agtggtggcc gatgcatacg gcggcgagtg cgttgggcgc gttgtagttg
     1621 ccgatgagct gcgtggcgat gtggtagtgc ccgtgttcgc gttggttgtc atcaaaaacc
     1681 agttccatgc agggttctgc gctggctacg gcgttcccgc gaacgttcag gccttccgcg
     1741 ttgccgtacg tgatgcgggc cagtccttcg ctcttggcca tcagcaacgg atcatcgccg
     1801 ttgacgaagc cggtgccgcg gtgtgcacgc agccattcgt acagctcggt cttggtggcg
     1861 atcacgcctt ccacgctaac gaagccctcg aggtgggctt ttccgatgtt ggtgatcaag
     1921 ccatgggtgg gctcggcgat ggcgcacagt tcggcgatat ctccgcgctt gctggcgccc
     1981 atctcgatga cggcgatgcg gtgctcggcc ttcaatttca gcagggtgag cggcacgccg
     2041 atgtggttgt tgaggttgcc ttcggtggcc agggtggggc ggtcggcggc aagcacggca
     2101 tggaccagtt ctttggtggt ggttttgccg ttggtgccgg tgacgccgat cacggggatg
     2161 tcgaacgtgc ggcggtggtg acgccccagg tcctgcaacg cctgcagcac atcgggcacc
     2221 aggaggtacc tctcgtcgcg caccacggcc gggtcgtcca ccacggcgta acgggcaccc
     2281 ttggcgagcg cgtcggcggc gaaggcattg ccatcgcgct tgtccttgag ggcgaagaac
     2341 atctcaccgg ctcccagtgc gcgggtatca taattggcac cgctgcattc caggtatgcg
     2401 gtgtgcaggc gggtgatgtc ggtcatggca caaatgaacg atcccccgcc ctggcggggg
     2461 atcgtccggt ggggaaggtt gaacgatccc gcgttacttc ttcagcttgc tgcggcgctt
     2521 gtcatcgccc aggcccacgg ggctgcccac gcgcaccatg gcgcagcgga agccgatggt
     2581 ggccgtgctc tggcgctcgt cgagatagcg gcgggtgccg gggttggccc agtaaatgcg
     2641 gtcggcccag ctggcacctt tgtacacgcg ggcgtgatcg ctgatcaggg tgaggtagtc
     2701 ctggaacgcc acttcgccgg agttggcgta catcccgccg ttctccggtt cgtccccttg
     2761 caggtagtac acgctgcttt ccaggtcgcc gtccttgaag tcgatgttgt cgctttcctg
     2821 gtagttgcgg cggtcgatgt tctcttccac ggtcactttg cgcatctgca cctcccccgg
     2881 ttggtcgctc tggtagtcgc tgatgcctga gagcagtttg gggcagatct ccaggtcctg
     2941 gcccttgatc atgtccacca tgtcctgcac gttctgctgg gcgggatcgg gcttgcgggt
     3001 ggaggcgaac tcctgggcct ggttgatctt ggtcatcagg tcctccacca gtttgccctc
     3061 ttcgtccgtg aacttgccct ccatgcgctt ctggaactcc tcgagccagt atttgatacc
     3121 atcgatatcg tagatcacct tgtcgtactt gtccttgatg ccgccatcgc tgttgagcac
     3181 cttggtcttg aacacgttgc cgcggaaggg gcggaagtcg ttctcgtcct caccggtgag
     3241 ggggcggtac acgtccatca cccactcgct cacgttgccg gccatgttgt agaggccata
     3301 gtcgttgggc cagtagctga acaccggcgc ggtgacatcg gcgttgtcgt tcagactgcc
     3361 ggccacgccc atgtaatcgc cgcggccgcg catgaagttg ccgcggaagt cgccgtaaaa
     3421 ggcaccgccc ttcttgcggc tgtcataacg cacccagtgt ccgttccagg ggtaaatgcg
     3481 gcgctccacg atgcgctcgt ccaccgtgtt gcccacgagt ccgtacgacg cgtattccca
     3541 ctcggcctcg gtgggcaggc ggtagcgggg aaggagcacg ccgtcctcca tcttgatgtt
     3601 gcgcgtgtcc ttgttcgggt tgaaatcctg cacgccatcc accttcttgc cgctctcgta
     3661 ctggccggcc aggtagctgt cggtggtgaa atgatcctcg ttgatctggt tgggataatg
     3721 ctcaaaaagc ccctcgcgga tcaggatcac ctcgttcacg cggtcggtac gccaggcgca
     3781 gtagtcgttg gcctggagcc agttgacgcc caccacggga tagtcgcggt aggccgggtg
     3841 ccgcaggtag tattcggtat agggctcggt gaaggcgagt ttgctgcgcc aaacgagcgt
     3901 gtcgggcagg gccttcttgt acacctcggg gaaatcggcg ccgtacacgc ggtccagcca
     3961 gtagaggtat tcgagccagt agaaattggt cacctccacc tcatccatg
//
LOCUS       DAOQOC010000120         3976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10409_length_3976_cov_3.820454, whole genome shotgun sequence.
ACCESSION   DAOQOC010000120 DAOQOC010000000
VERSION     DAOQOC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10409_length_3976_cov_3.820454"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PL106_02945"
     CDS             <1..190
                     /locus_tag="PL106_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNA32055.1"
                     /translation="VSPEMISAVRKGVELPIIVGGGIRDAATARTLCEAGADVLVVGT
                     AFEQDPELIFTMSEAVHG"
     gene            complement(211..1071)
                     /locus_tag="PL106_02950"
     CDS             complement(211..1071)
                     /locus_tag="PL106_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32056.1"
                     /translation="MARAVFFLAIVLGALTSNGQFHALPQGHAIWAVSFWIGPGYPYE
                     LSMYDLDPAAPDTVISGVSYDLLGGTPVRDDLAGRVFFWDVGSAEEKLLYDFDVLPGD
                     TILYPPGVVDGDTIHVIGVDTVDVAGTPRKQITVSLYQPGSWGECVWIQGIGSTGGPF
                     NVCQGPSVSGTSWLTCMTEDGIFQFGGAVGQPGDCSMYYSTGPEHAHREFMKVHPNPG
                     EDELFIERSSAGVAELSITGPDGRALNLQPIRSERLKVDMSHRPPGVYLITIKDASGS
                     SRSVVWVKEP"
     gene            1176..3125
                     /locus_tag="PL106_02955"
     CDS             1176..3125
                     /locus_tag="PL106_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA32057.1"
                     /translation="MRPFLSIISLVFFCGAFAQSDSLWSVWKNSSLPDSARLKAISAL
                     SWKTVFQQPDSGIALAELQLQLARKSGDPKALFTAYNTLAAGNKMRSDLSAALDYFNR
                     CLEVARSMNDRKRIANTLSNLSTIYKDLGDQPRALQLLHQSLRIDEELGNKEGMAGTF
                     NNIGNIYKRVNETAKALENYAKSAALYAELDNPRGRAVVLVGIGTLHGDLGMLDTAVA
                     ELRTAIGLYHRIGNRLDLGKAHNNLGQVLTRMGRMREAYAEFDSARTIHGSMGARDLL
                     ARNLYYTGQALLREGRAAEAVRSCREGLAIADSLGLLDQRKECTDCLMHAYAQAGDFR
                     RAYAMQQAFIDLDDTLDRANNGKEVTRLEMTRVFRERWIADSLDQVRTDLEKQMVVDR
                     RMAEQRTQRGFLIFSVIVALVIAGALLSRLRYTSKAKRAIEREKERSDDLLHNILPEE
                     VAAELMEKGSAQARTFDSATVLFTDFKGFTAMSEQLTATELVAEIDHCFKALDAIVEK
                     HGVEKIKTIGDSYMAAAGLPDPASSNAADIVLAALEMRDAIAQRRAERLREGRPAFEM
                     RIGLHSGPVIAGIVGVKKFAYDIWGDTVNTASRMESSGEPGRVNISAATHALVKDVIW
                     LRFEPRGMVAAKGKGALEMYFVDRA"
     gene            complement(3182..>3976)
                     /locus_tag="PL106_02960"
     CDS             complement(3182..>3976)
                     /locus_tag="PL106_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32058.1"
                     /translation="CYNFEVFDSGNNGFGESRDAGYAKLMSLGADVAVATGNFGGLYR
                     KVAETGSDDGCMPTQLVSTPDPVISCNTWNHILGQSTLYADDVPGANLYRFRFTNVAG
                     QPAYARNITSPGRALPLNKWYTLPLKPGRTYHVQIQASFNNGASYCAYGSPCLIHIGY
                     PQLGQGEMRAAQAELLPVDRLFSVFPNPSADGRFNLRADGFDAEEPLNIEVLTLQGAR
                     VAAPRTLVLAEEGVQALALDRPLESGMYILRITVGGSVTTERLIVR"
BASE COUNT          825 a         1232 c         1230 g          689 t
ORIGIN      
        1 ggtgagcccg gagatgatca gcgcggtgcg caagggcgtg gagctgccga tcatcgtcgg
       61 cggtggcatc cgcgatgcgg caacggcacg cacgctctgt gaggctggtg ccgacgtgct
      121 ggtggtgggc acggccttcg agcaggatcc cgagctgata ttcaccatga gtgaggcggt
      181 gcatggatga gtcccatcct cgagtctctc ctacggctcc tttacccaga ccacggacct
      241 cgaggaacca gaggcgtcct tgatcgtgat gagatagacc ccgggtggcc gatggctcat
      301 atcgactttc aaccgttcgg atcgtatcgg ctgcaggttg agggcacgtc cgtccggccc
      361 ggtgatggag agctccgcga cccctgcact cgaacgctcg atgaacaact cgtcttcgcc
      421 tggattcgga tgaactttca tgaactcacg gtgcgcatgc tctgggccgg tgctgtaata
      481 catggagcaa tcgcccggtt gacccaccgc tccgccaaat tggaagatcc catcctccgt
      541 catgcaggtg agccaacttg ttccggagac cgaaggaccc tggcagacat tgaacggccc
      601 gccggtgctt ccgatgccct ggatccaaac acattcgccc caggatcctg gctggtacaa
      661 agagaccgtg atctgtttgc gaggtgttcc ggccacatca acggtatcga cgccgatgac
      721 gtggatcgta tcaccatcta ccacgccggg agggtagagg atcgtgtcgc ccggcaggac
      781 atcaaagtcg tacagtagtt tctcctctgc cgatccaacg tcccaaaaga aaacccggcc
      841 ggcaaggtca tcgcgaacag gggttccccc caacaggtca taggatactc cactgatcac
      901 ggtatcgggc gccgctggat ccaggtcgta catcgatagc tcgtacggat agccgggacc
      961 gatccagaat gagacggccc agatagcatg gccctgtgga agagcatgga actggccgtt
     1021 ggatgtgagt gcgcccagga cgatggccag gaagaaaacc gcacgtgcca tcactgacat
     1081 ggtgttggga cgatgaaagt actgaacatc cttcagacgt gccgggagcg cgcaccgctg
     1141 cccgcaaacc gacccgctat cttcacccgg tcctaatgcg tcccttcctt tccatcattt
     1201 ccttggtatt cttctgcgga gcgttcgcgc aaagcgattc tctctggtcc gtctggaaga
     1261 acagttccct ccccgacagc gcgcgcctga aggccatcag tgccctgtcg tggaaaacgg
     1321 tgttccaaca gcccgacagt ggcatcgccc tggccgagct gcagttgcaa ctcgcgcgca
     1381 agtccgggga ccccaaagcg ttgttcacag cgtacaacac gctggcggcg gggaacaaaa
     1441 tgcgcagcga cctgagtgct gcgctcgact atttcaaccg ctgcctggag gtggcgcgtt
     1501 ccatgaacga ccggaagcgc atcgccaaca cgctcagcaa cctgagcacc atttacaagg
     1561 atctgggcga tcagccccgg gcgctccaat tgctgcacca gagcctgcgg atcgatgagg
     1621 aactgggcaa caaggagggc atggccggca ctttcaacaa catcggcaac atctacaaac
     1681 gggtgaacga aacggccaag gccttggaga actacgcgaa gagcgccgcg ctgtacgccg
     1741 agctggacaa cccgcgaggc cgggcggtgg tactggtggg catcggcacc ttgcatggtg
     1801 acctgggcat gttggacacg gcggtggccg agctgcgtac ggccatcgga ttataccatc
     1861 ggatcggtaa ccggctcgac ctgggcaagg cgcacaacaa cttgggccag gtgctcacgc
     1921 gcatgggccg catgcgcgaa gcgtatgcgg aattcgacag tgcgcgcacg atacacgggt
     1981 ccatgggcgc gcgggacctg ttggcgcgca acctctatta cacgggccag gccctgctgc
     2041 gcgaaggacg cgccgccgaa gcggtgcgca gctgccgcga agggctggcg atcgccgaca
     2101 gcctcggtct gttggaccag cgcaaggaat gcaccgactg cctgatgcac gcatacgccc
     2161 aagcggggga cttccgcagg gcctacgcca tgcagcaagc gttcatcgac ctggacgaca
     2221 ccttggatcg ggccaacaac ggcaaagagg tcacccgttt ggaaatgacc cgtgtgttcc
     2281 gtgagcgctg gatcgccgac agccttgacc aggtgcgcac cgacctggag aagcagatgg
     2341 tggtggaccg caggatggcc gagcaacgca cgcaacgggg cttcctgatc ttctcggtga
     2401 tcgtggccct ggtgatcgcc ggcgccctgc tgagccggct acggtacacc agcaaggcga
     2461 agcgtgccat tgaacgggag aaggaacgca gcgatgacct gctgcacaat atcctgcccg
     2521 aggaggtggc cgccgagctc atggagaaag gaagcgctca agcacgcacg ttcgacagcg
     2581 ccaccgtgct ctttaccgat ttcaagggat tcacggccat gagcgaacag ctcaccgcca
     2641 ccgaactggt ggccgagatc gatcactgtt tcaaggcact ggacgccatc gtggagaagc
     2701 acggtgtaga gaagatcaag accatcggcg attcctatat ggccgccgcc ggacttccgg
     2761 atccggcatc gtccaatgcc gcggacatcg tactggccgc actggagatg cgcgacgcga
     2821 tcgcccagcg ccgtgccgaa cgcctgcgcg aaggccgacc cgccttcgag atgcgcatcg
     2881 gcctgcacag cggccccgtg atcgcgggca tcgttggcgt gaagaaattc gcctacgata
     2941 tatggggcga tacggtgaac acggccagcc ggatggagag cagcggcgaa cccggcaggg
     3001 tgaacatcag tgcagcgaca cacgcgctgg tgaaggatgt catctggctc cgtttcgagc
     3061 cgcgcggcat ggtcgcggcc aaaggcaaag gcgcactcga gatgtacttc gtggatcgcg
     3121 catgaccgac caccggcacc cggatcggta tttttacatc gcctcccctg cccgaccctg
     3181 ctcagcgcac gatcaatcgc tcggtggtca cactcccccc gacagtgatc cgcaggatgt
     3241 acatcccgct ctccaagggg cggtccagtg ccaaggcctg cacgccctcc tcggcgagga
     3301 cgagggtgcg cggtgctgcc acgcgggctc cctgcagggt gagaacctcg atgttcagcg
     3361 gctcttcggc atcgaaccca tcagcccgca ggttgaaccg tccatcggcc gaagggttcg
     3421 ggaacaccga gaacaaccgg tcaacaggca acagctcagc ctgcgctgcg cgcatttcgc
     3481 cctggcccag ctgcgggtac ccgatatgga tcaggcacgg ggatccatag gcgcagtagc
     3541 tggccccgtt attgaagctc gcctgtatct gcacgtgata ggtacggccc ggcttcaagg
     3601 ggagcgtgta ccacttgttc agcggaagcg cgcggccggg tgaagtgatg ttgcgcgcgt
     3661 aagcgggttg acccgccacg ttggtaaagc ggaaccggta caaattcgca ccgggcacat
     3721 catcggcgta cagcgtggac tgaccgagga tatggttcca cgtgttgcag gagatcaccg
     3781 gatccggcgt gctcaccagt tgcgtgggca tgcagccatc gtccgagccc gtttcggcca
     3841 ccttgcgata gagcccaccg aaattgcccg tggccacggc cacgtcagcg ccaaggctca
     3901 tcagtttggc ataacccgca tcgcgcgact cgccgaatcc attgttccca ctgtcgaaca
     3961 cctcgaaatt gtagca
//
LOCUS       DAOQOC010000121         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10498_length_3957_cov_3.628139, whole genome shotgun sequence.
ACCESSION   DAOQOC010000121 DAOQOC010000000
VERSION     DAOQOC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10498_length_3957_cov_3.628139"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PL106_02965"
     CDS             <1..1730
                     /locus_tag="PL106_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNA32059.1"
                     /translation="FDLSFALAQGGALKGGFMIDSEHSVYAIDAGLTGFALPQLLPYL
                     QDLVRTTALTGQVDLNLHLRDSWVASDGLAVRADLGLRDLGVTDANGEPLVGIRSARV
                     KLDTLDARSDRFDLTSVAVDGFSTRFQLWPDGSDTWSRALKLDTMAAGGDTTAVLTAS
                     SDNIFVMLADWIRTLGRDLVANEYNADSLAITNAELVFEDHTPERPFRYVLNDMVVRS
                     SRMNSGGDSLRLGASATLNGRGQLSSSFAFDPQNLKNVDAMLQVSDLALADLDAYGRW
                     YAAHPLEEGTLDYMGRTRIADGRLNSANHLRARGMQVGKKIDAHDPGIVVLPLRLGVS
                     LLKDVKGDIDLDIPVEGDINDPKFKPWPIVWKVVKNLVVKAATAPGRMLVRAVGGGDA
                     NELDKVRFEPLETTLGKEQRRTLEQLAKGLKAKPDVDVALVPLGDQQQELEALAAFEV
                     KKTFLGYTGALVAVDSARINALSTRDSSFVGYLNERSPTTVGQGEHQRCVALLGGGTL
                     QSRCVEMEQARQKAVRNFLLSQGLAEDRFTIRQGTVEETRGYVGKPSYRLIFDAGAKA
                     LDRAGPSAR"
     gene            1859..1932
                     /locus_tag="PL106_02970"
     tRNA            1859..1932
                     /locus_tag="PL106_02970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1893..1895,aa:Asn,seq:gtt)
     gene            1994..2923
                     /locus_tag="PL106_02975"
     CDS             1994..2923
                     /locus_tag="PL106_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNA32060.1"
                     /translation="MQLITVGTVAFDRIETPFGTAEKTVGGAATYISLAASIFLDKMG
                     LVSVVGDDFPVPVMRQLEKRGVDLSGLEILKGKESFFWAGRYHYDMNSRDTLETRLNV
                     LLELDPKLPASYRKAPYVMLGNLDPVVQGKVLDQMDERPALVVMDTMNFWMDSALDKL
                     KEVIRRVDILTINDAEARQLSGEHSLVKAAARILTMGPKYLVVKKGEHGALLFGAGRV
                     FFAPALPLEDVIDPTGAGDTFAGGFIGYLARTQDHGFDNLKRAIIVGSALASFTCEKF
                     GIDRLVEVSREEVDARIQQFVELVDFDIELVEE"
     gene            complement(2925..3506)
                     /locus_tag="PL106_02980"
     CDS             complement(2925..3506)
                     /locus_tag="PL106_02980"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNA32061.1"
                     /translation="MALPYAYDALEPMIDKQTMEIHYTKHHAAYVKNANAAIAEEGVK
                     APDEAALLAGISAYSTKLRNNAGGVWNHNFFWQVMGPGSKSAEGSKVLDALNGAFGSF
                     DKFKEVFTDAAMKRFGSGWAWLVQKDGKLEVGSTPNQDCPLMDVCEFKGRPLLGLDVW
                     EHAYYLHYQNKRNEYVAAWWNTVDWNALAARLG"
     gene            complement(3739..>3957)
                     /locus_tag="PL106_02985"
     CDS             complement(3739..>3957)
                     /locus_tag="PL106_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNA32062.1"
                     /translation="GAAVKEIKAPMPGLVLKVLVKEGDAVKKNEPVLILEAMKMENVI
                     KSPGDGTVKRIHAQEKTAVEKGQLLIGF"
BASE COUNT          710 a         1185 c         1305 g          757 t
ORIGIN      
        1 atttcgacct ttcgttcgcc ctggcccaag ggggtgccct gaagggcggg ttcatgatcg
       61 attcggaaca ttcggtctac gccatcgatg ccggcctgac cgggttcgcc ctgccgcaac
      121 tgcttccgta cctgcaggat ctggtgcgca ccaccgcgct cacgggccag gtggacctga
      181 acctgcacct gcgcgacagt tgggtcgcca gcgatggtct ggccgtgcgg gccgatctcg
      241 gtctgcgcga cctgggcgtc accgatgcca acggcgaacc cctcgtgggc atccggtccg
      301 cgcgggtcaa gctggacacg ctggatgcca ggagtgacag gttcgacctg acaagcgtcg
      361 cggtggacgg cttcagcacg cgcttccagc tgtggcccga tggttcggat acctggagca
      421 gggcattgaa gctggacacc atggccgcgg gaggcgacac caccgccgtg ctcacggcca
      481 gcagcgacaa catcttcgtg atgctggccg attggatccg cacgctgggt cgggatctgg
      541 tggccaacga gtacaatgcc gacagtctgg ccatcaccaa cgccgaactg gtttttgagg
      601 atcatacacc ggagcgcccg ttccgctacg tgctcaacga catggtcgtg cgcagcagca
      661 ggatgaacag tggcggggac agcctgcgcc tgggcgcatc ggccacgctc aacggccggg
      721 gccagctctc cagctccttc gcattcgacc cgcagaacct gaagaacgtg gacgccatgc
      781 tccaggtgag cgacctggca ctcgccgacc tggatgccta tggccgatgg tacgccgcac
      841 atccactgga ggaaggcaca ctggactaca tgggccgcac ccggatcgcg gatggtcggt
      901 tgaacagcgc gaaccacctc cgggcgcgag gcatgcaggt gggcaagaag atcgacgcgc
      961 atgaccccgg gatcgtggtg ctgccgttgc ggctgggcgt gagcctgctc aaggacgtga
     1021 agggcgacat cgacctggac atcccggtgg agggcgacat caacgacccg aagttcaagc
     1081 cctggcccat cgtgtggaaa gtggtgaaga acctggtggt gaaggccgcc acggcgccgg
     1141 gccgcatgtt ggtgcgcgct gtgggcggtg gcgatgcgaa cgagctggac aaggtgcgat
     1201 ttgagccctt ggaaaccacg ctgggcaagg agcagcggcg cacgctggag caactggcca
     1261 aaggcctcaa ggccaaaccg gatgtggatg tggcgctggt gccgttgggc gaccagcagc
     1321 aggagctgga agcactggct gcctttgagg tgaagaagac cttcttgggc tacacgggcg
     1381 ctttggtggc ggtggacagc gcacggatca atgcgctgag cacgcgtgac agttcgttcg
     1441 tcgggtattt gaacgagcgt tcgcccacca ccgtgggcca aggcgagcat cagcgctgcg
     1501 tggccttgtt ggggggcgga acattgcagt cccgttgtgt ggaaatggaa caggctcggc
     1561 aaaaggcggt gcggaacttc ttgctgtccc aaggactggc cgaagaccgc ttcaccattc
     1621 gccaaggcac ggtcgaggag acccggggat acgtgggcaa gcccagctac cgcttgatat
     1681 tcgatgcggg ggccaaggcg ctggaccgtg cggggccatc ggcccgatga ggggttgggc
     1741 catcgcgcat tctgcgaggt gctgcccctt ggagcacccc gctcatggct cccggacatg
     1801 ttggcgcttg tcccgatcgc ccatccgcct acatttgccg tccctttcga ggggaccttc
     1861 cctggtagct cagctggtta gagcacatga ctgttaatca tggggtcgct ggttcaagtc
     1921 cagcccgggg agcagaagag agccgcaagc gcggctttct ttgtttactg aaggaaccga
     1981 acgcattcaa gccatgcaac tcatcaccgt aggcaccgtc gcttttgacc ggatcgaaac
     2041 ccccttcggc acggccgaga agaccgtggg cggggcggcc acctacatct ccctggccgc
     2101 atcgatcttc ctggacaaga tggggctggt gagtgtggtg ggcgacgact tccccgtacc
     2161 cgtgatgcgg cagctggaaa agcgcggggt ggacctgagc ggcctcgaga tcctgaaggg
     2221 caaggagagt tttttctggg ccggacgcta ccactacgac atgaacagcc gcgatacgct
     2281 ggagacacgg ctgaacgtgc tgttggaact ggacccgaag ctgcccgcct cctaccgcaa
     2341 agcgccttat gtgatgctgg gcaacctgga ccccgtggtg cagggcaagg tgcttgacca
     2401 gatggacgag cgccccgcat tggtcgtgat ggacaccatg aacttctgga tggattcggc
     2461 gctggacaaa ctcaaggaag tgatccgccg ggtggacatc ctcaccatca acgatgcgga
     2521 ggcgcgccag ttgagcgggg agcacagtct ggtgaaggcc gccgccagga tcctgaccat
     2581 gggaccgaaa tacctggtgg tgaagaaggg cgagcacgga gcactgctgt tcggggccgg
     2641 ccgtgtgttc ttcgcacccg cgctgccctt ggaggacgtg atcgatccca ccggtgcggg
     2701 cgacacgttc gccggtgggt tcatcggcta cctggcacgc acgcaggacc acgggttcga
     2761 caacctgaag cgcgccatca tcgtgggcag tgcgctggcg agtttcacct gcgagaagtt
     2821 cggtatcgat cggctggtcg aagtgagccg cgaggaggtg gacgcccgca tccagcagtt
     2881 cgttgaactg gtggatttcg atattgaact ggtggaagag tagttcagcc aaggcgggcg
     2941 gccagggcgt tccagtccac cgtgttccac caggcggcca cgtattcgtt acgcttgttc
     3001 tggtagtgca ggtaataggc gtgctcccac acgtccaggc ccagcagggg tctgcccttg
     3061 aactcacaca cgtccatcag ggggcaatcc tggttggggg tgctgcccac ttccagtttg
     3121 ccgtccttct gcaccagcca ggcccagccg ctaccgaagc gcttcatggc cgcatcggta
     3181 aaaacctcct tgaacttgtc gaaggagccg aatgcgccgt tgagggcatc gagcaccttg
     3241 cttccctcgg cactcttgct ccccggcccc atcacctgcc agaagaagtt gtggttccac
     3301 actccaccgg cattgttgcg gagcttggtg ctgtacgcgc tgatgcctgc gagcaatgcc
     3361 gcctcgtccg gggctttcac tccctcttca gcgatggccg cattggcgtt cttcacatag
     3421 gccgcgtggt gcttggtgta gtggatctcc atggtctgct tgtcgatcat gggctccagg
     3481 gcgtcgtatg cgtagggcag cgcgatctgt gcgagttgca aggcgggggt gggaggtgat
     3541 gcgggatccg ggggcgtggt gttctccgcc tcggcggggg tattgcacgc ggccatgttg
     3601 gccaaggcgg gcccggccac gatcgccccc aacgaggcct tcagcgatcg cgaaaggaat
     3661 tgtttgcggt ccattgctcg gttcaccatt ttgaagcatg gcgatgggcc gaaggtactg
     3721 cgcctggatg cggacccgtc agaagccgat cagcagttgt cctttctcca cggcggtttt
     3781 ttcctgtgcg tggatgcgct tcaccgtgcc gtccccgggg cttttgatca cattctccat
     3841 cttcatggcc tccaggatca gcacgggttc gttcttcttc acggcatcgc cctccttcac
     3901 cagcactttg agcaccagcc cgggcatcgg cgctttgatc tccttcacgg ctgcacc
//
LOCUS       DAOQOC010000122         3953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10519_length_3953_cov_2.407132, whole genome shotgun sequence.
ACCESSION   DAOQOC010000122 DAOQOC010000000
VERSION     DAOQOC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10519_length_3953_cov_2.407132"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PL106_02990"
     CDS             complement(<1..1094)
                     /locus_tag="PL106_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNA32063.1"
                     /translation="MSVKIAEAPATQGVLGRMENNDHEEVLFAFDRITGLRAIIAVHD
                     TTLGPALGGTRMWPYETEAEALNDVLRLSRGMTYKSALAGLDLGGGKAVIIGDARTQK
                     TEAMFRRFGRFVDSLNGRYITAEDVGMSTADMVNIRKETRHVAGLPIEVGGSGDPSPV
                     TAYGVFCGMRAAAKTAFGKDSLSGRKVAIQGGGNVGRHLAGYLVKDGAIVFITDIHED
                     KLAAIKAEHPAVNLVKPDAIYGLDVDIYSPCALGATVNDETLAKLKCSVIAGAANNQL
                     ADEKKHGVAVMEKGMLYAPDFLINAGGIINCAWERKGYDRNAAMKQTENIYATTLEIF
                     KRSKAENIPTYLAANQAAERRVLSIKQAGLR"
     gene            1363..2583
                     /locus_tag="PL106_02995"
     CDS             1363..2583
                     /locus_tag="PL106_02995"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNA32064.1"
                     /translation="MRTFSIPTHYRSDLIGRLKAWRKVQDPRKKDLSPTLLDLGAVRF
                     VFARHFGFCYGVENAIEISYQALEQNPGKRIYLLSQMIHNPEVNADLESRGLRFIQDT
                     HGRMLMDWSELRSDDIVIIPAFGTTLETEARLKAIGIDPLRHNTTCPFVEKVWKRSAQ
                     LGAKDYTVVIHGKAAHEETRATFSHTAAGAPAVIVKDMDEARLLGRIISGEEPMGRFT
                     EIFGERATPGFNPVNDLQRIGVVNQTTMLATETQAIADHLKQVMIHKYGDASTGDHFA
                     DTRDTLCYATNDNQDATHELLKAEADLALVVGGYNSSNTSHLVELLEHRFPTYFINGE
                     KELVSATEIEHFNYPLQKLERTRQWLPAKRPVTIILTSGASCPDTLLDRVMLKVLSMV
                     DGVKDPVDAVEGMK"
     gene            2708..3730
                     /locus_tag="PL106_03000"
     CDS             2708..3730
                     /locus_tag="PL106_03000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA32065.1"
                     /translation="MRVPVLPCAIIACLVSMAAHAAQPNRLSGRIIAPTGDSVFVQQA
                     VVVGGKASERTLAAAKLDANGGFRMAFDLDSAQALTFFDGNEITQFFALPGEQQELTL
                     HTAYFDESLYFAGDGAGRNNALAGLALANENEWNRVAQDAADTAHADEQVDALVQRLA
                     KVMDEYAVAYPELANLLAERKAADLQGAAQQKQWMQSRILFKKRVVEMVGQPMTNLMG
                     RDLDGGKLDLARYKGRITVLDFWATWCGPCKAEMPAWEALQKEYGDRINFVSISVWDN
                     EDKWKEMSKSLGHAHSMFIPKTDLGQLEPYMVNAIPRYMVLDKDLRIVTIDAPRPSSG
                     ELQKFF"
BASE COUNT          785 a         1218 c         1216 g          734 t
ORIGIN      
        1 cggaggccgg cctgcttgat gctgagcacg cgccgttcgg cggcctggtt ggcggccagg
       61 tacgtgggga tgttctcggc cttgctgcgc ttgaagatct ccagcgtggt ggcgtagatg
      121 ttctcggtct gcttcatggc cgcgttgcga tcgtagccct tgcgctccca ggcgcagttg
      181 atgatccccc ccgcgttgat caggaaatcc ggagcataga gcatgccctt ttccatcacg
      241 gccacgccat gctttttctc gtcggcgagc tggttgttcg ccgcacccgc gatcacgctg
      301 cacttgagct tggccaacgt ctcgtcgttc accgtagcac ccaaggcgca cggtgagtag
      361 atgtccacgt ccagcccgta gatggcatcg ggcttcacca ggttcacggc gggatgctcg
      421 gccttgatag cggccagctt gtcctcgtga atgtcggtga tgaagactat tgccccatcc
      481 ttcaccagat agcccgcaag gtgcctcccc acgttgccgc cgccttggat ggccactttc
      541 ctgccgctca ggctgtcttt gccgaaggcg gttttcgcgg cagcacgcat gccgcagaac
      601 acgccgtacg ccgtcacggg cgaagggtcg ccgctgcctc ccacttcgat gggcaggccg
      661 gccacgtgcc tggtttcctt gcggatgttc accatgtcgg ccgtgctcat gcccacgtcc
      721 tccgcggtga tgtagcggcc attcaggcta tccacgaaac gaccgaagcg gcggaacatg
      781 gcctcggtct tctgggtacg cgcgtcgccg atgatcacgg ccttgccacc gccgaggtcc
      841 aagccggcga gggcactctt gtacgtcatg ccacggctca ggcgcagcac atcgttcaac
      901 gcttcggcct ccgtctcata gggccacatc cgggtgccac cgagggccgg gcccagggtg
      961 gtatcgtgaa cggcgatgat ggcgcggagc ccggtgatgc ggtcgaatgc gaaaaggacc
     1021 tcctcgtggt cgttgttctc catacggccc aacacacctt gggtggcggg cgcttcagcg
     1081 attttcacgg acatgctaca aagtgttgtg gagcgcgatc ccggggtttg ggcacgcaat
     1141 gggtgatcga tcggccggca ggagcagcgc tgggaaatgg tgcggatgcg tcatgaggtg
     1201 aggccgggtg cgaagaacgt acatcccgtg gtcggatgcg gtgacaaatg tagccgtggt
     1261 ccctgaggcg gcaccatgaa caagtcatga aaatgccgcg tggcccggcc cggctaccga
     1321 tagctttgcc ccttcacatc cggtccctcg gccggtggcc ccatgcgcac cttcagtatc
     1381 cccacgcact accgcagcga cctgatcggt cggctcaaag cttggcgcaa ggtgcaggac
     1441 ccgcgcaaga aagacctctc ccccacactg ctggatctgg gggcggtccg tttcgtcttc
     1501 gcgcggcact tcggattctg ctacggagta gagaacgcca tcgagatcag ttaccaagcc
     1561 ctggagcaga accccggcaa gcggatctat ttgctgagcc agatgatcca caaccccgag
     1621 gtgaacgccg atctggaaag tcgcgggctg cgtttcatcc aggacacgca cggccgcatg
     1681 ctgatggatt ggagcgaatt gcggagcgat gacatcgtga tcataccggc cttcgggacc
     1741 accttggaga cggaggcacg gttgaaggcc atcgggatcg atccgctgcg acacaacaca
     1801 acatgcccgt tcgttgaaaa ggtgtggaag cgcagcgccc agctcggggc gaaggactac
     1861 accgtggtga tccatggcaa ggccgcgcac gaggagacgc gggccacgtt cagccacacc
     1921 gcggccggag cacccgccgt gatcgtgaag gacatggacg aagcacgatt gctcgggcgc
     1981 atcatctcgg gcgaggagcc catggggcgt ttcacggaga tcttcggcga gcgggccacc
     2041 cccggtttca acccggtgaa cgacctgcaa cgcatcgggg tggtgaacca gaccaccatg
     2101 ctggccaccg agacccaggc catcgccgac cacctcaaac aggtgatgat ccacaaatac
     2161 ggggacgcat ccacgggcga ccatttcgcg gacacgcggg acacgctctg ctacgccacc
     2221 aacgacaacc aggatgcgac ccacgaattg ctgaaagcag aggccgacct ggccttggta
     2281 gttggcggat acaacagcag caataccagc cacctggtgg aattgctcga gcaccgcttc
     2341 cccacctact tcatcaacgg cgagaaggaa ctggtgagcg ccacggagat cgagcacttc
     2401 aactatccgt tgcaaaagct tgaacggacg cggcaatggc tgccggcgaa gcgaccggtc
     2461 acgatcatcc tcaccagcgg ggccagctgt ccggacacct tgttggaccg cgtgatgctg
     2521 aaggtcctat cgatggtcga tggggtgaag gacccggtgg atgccgtgga gggtatgaaa
     2581 taggtgatcg cggtagccgt ccgatcgttg ccctcgaatt cccgccattg cgcatgtgca
     2641 aagcgatggg cgtgatacat tcggcggcac ccaacgccgg atccacttcc caccttcccg
     2701 acccatcatg cgtgtaccgg tcctcccttg cgccatcata gcctgccttg tatcaatggc
     2761 cgcgcatgcg gcacagccca atcgcctttc gggacgcatc atcgcaccta cgggtgatag
     2821 cgttttcgtg caacaagcgg tggtggtggg cggaaaagcc agcgagcgaa cattggcagc
     2881 ggccaagctc gacgcgaacg gggggttccg catggccttc gatctggaca gtgcacaggc
     2941 actcaccttc ttcgatggca acgagatcac ccaattcttc gccttgcccg gtgaacaaca
     3001 ggagttgacc ctgcatacag cctacttcga tgaatcgctg tacttcgccg gcgacggggc
     3061 tggccgtaac aacgccctcg ccggattggc cctggccaac gagaacgagt ggaaccgggt
     3121 cgcgcaggat gcagccgaca ccgctcacgc ggacgaacag gtcgatgcac tggtacaacg
     3181 cctggccaag gtgatggatg agtacgcggt cgcctatccc gaattagcga acctgctcgc
     3241 cgagcggaag gccgctgacc tccagggagc cgcacaacag aagcagtgga tgcaaagtcg
     3301 gatcctcttc aagaagcgcg tggtcgaaat ggtggggcag cccatgacca acctgatggg
     3361 acgcgacctc gacggcggaa agttggacct cgcacggtac aaaggccgca tcaccgtgct
     3421 cgacttctgg gccacctggt gcggaccctg caaagcggag atgcccgctt gggaagcgtt
     3481 gcagaaggaa tatggcgacc ggatcaactt cgtgagcatc agtgtatggg acaacgaaga
     3541 taaatggaag gagatgtcga agtcgttggg tcatgcccat tccatgttca tcccgaagac
     3601 cgaccttgga cagttggagc cgtacatggt caacgccatt ccgcgttaca tggtgttgga
     3661 taaagacctg aggatcgtga ccatcgacgc gccacggcct tcatcgggcg aattgcagaa
     3721 gttcttctga aggtgggctg gcccggctac cgatagcttt gcctctatgc atccggtcac
     3781 ttgaccggtg gcccatgcgc accttcagca tccccacgca ttaccgcagc gacctgatcg
     3841 gtcggctcaa agcctggcgc atggtgcaag acccggccga ggtggtgaag gccatggccc
     3901 gatgaggcgt catctacccc tgttcctgtt ctttttcgtg ccgtgcatgc tcc
//
LOCUS       DAOQOC010000123         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10602_length_3935_cov_3.736082, whole genome shotgun sequence.
ACCESSION   DAOQOC010000123 DAOQOC010000000
VERSION     DAOQOC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10602_length_3935_cov_3.736082"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PL106_03005"
     CDS             complement(<1..347)
                     /locus_tag="PL106_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNA32066.1"
                     /translation="MSTLRTFDLAGLSIPQRHGLLLGAVGPRPIAFASTIDALGRPNL
                     SPFSFFNCFGSNPPIVVFSPARRGRDNTTKHTLHNVRAVPEVVINVVDFGMTQQANVA
                     SGEYGEGVNEFVKA"
     gene            437..518
                     /locus_tag="PL106_03010"
     tRNA            437..518
                     /locus_tag="PL106_03010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:471..473,aa:Leu,seq:tag)
     gene            706..2061
                     /gene="tig"
                     /locus_tag="PL106_03015"
     CDS             706..2061
                     /gene="tig"
                     /locus_tag="PL106_03015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNA32067.1"
                     /translation="MNIEREETGTLTATLKLKLLPEDYAPAVEKTLKEQRRTAALPGF
                     RPGQVPMAIIKKRIGRNVLVNEVERVIERSLNGYITENRLRPLGQPLPKNENFSANNW
                     DEPGEFNFAYEMGLAPVFDVELNEKLGVEMPVVDVDDALVNKEVADMQRRFGQVVDVE
                     RAADKDMLLGDLIELDEAGAIKPGGLMNRTTISLEYLKDEEARRLFVGKAKGDEVKVD
                     PHRISDGHDDLARMLGADHEAVHGLQGDMLFRVEAIKRMEAVPLDQTLFDRVYGQGAV
                     TDETACRAKVKAGLENAFRRDSDRVFKRLVMKKLQENAHIELPDEFLKRWIRTTSEKP
                     ITPEQVEEGYTSYATGLKRQLVEDRVVEKYGLEAKGEEIDGFAKRYISDQFVQYGMEA
                     PEGEALQAAAARLLGDRDQIKRMRDAIVEQKLLVHFKNMVSPKERRLPFDAFVNLART
                     A"
     gene            2092..2754
                     /gene="clpP"
                     /locus_tag="PL106_03020"
     CDS             2092..2754
                     /gene="clpP"
                     /locus_tag="PL106_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNA32068.1"
                     /translation="MIPNDEFRKYAVKHRGINSLVLRDYQASMTPYIIEERQLNVAQM
                     DVFSRMMMDRIIFLGSGINDQVANIVQAQLLFLESLDAKKDIQIYMNSPGGGVYAGLG
                     IYDTMQYINPDVATICTGMAASMGAVLLCAGAKGKRSALKHARVMIHQPMGGAEGQAS
                     DMEITVNEIKKLKKELYEIIANHSGQPFERVEKDGDRDYWMIAEEAKAYGMIDELLVR
                     QK"
     gene            2942..>3935
                     /gene="clpX"
                     /locus_tag="PL106_03025"
     CDS             2942..>3935
                     /gene="clpX"
                     /locus_tag="PL106_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNA32069.1"
                     /translation="MDKKEIKCSFCGRDKQDTNVLIAGITGHICDSCIGQAQNIIHEE
                     LSHRDRNEPDGGIILQKPSDIKRYLDEYIIGQDDAKKVLSVAVYNHYKRLMQKSTGAD
                     DVEIEKSNVILVGETGTGKTLLARTIAKMLNVPFAIADATVLTEAGYVGEDVESILSR
                     LLQAADYDVTKAERGIVFIDEIDKISRKSDTPSITRDVSGEGVQQALLKLLEGSTVNV
                     PPQGGRKHPEQKLIQVDTRKILFVCGGAFDGINKIIARRVKSSSVGYSASKENRVLDE
                     NLLQYVAPTDLRSYGLIPELIGRFPVLTYLDPLDRETLRRILTEPKNALIKQYVK"
BASE COUNT          897 a         1148 c         1231 g          659 t
ORIGIN      
        1 gctttgacga attcgttcac gccttccccg tactcgccgc tggccacatt ggcctgttgc
       61 gtcatgccga agtccaccac gttgatcacc acttcgggca cggcccgcac gttgtgcagc
      121 gtgtgtttcg ttgtgttgtc gcgaccacgc cgggcgggcg agaacacaac gatgggcgga
      181 ttgctgccga aacagttgaa gaagctgaag gggctgaggt tgggccgccc aagcgcatcg
      241 atggtgctgg cgaaagcgat gggccgggga cctacggccc ccaacaaaag gccgtgccgt
      301 tgggggatgg acagcccggc caggtcgaag gtgcgcaggg tgctcatggg atgggggacg
      361 cgaatgtaga gccccgtggt ccggtccggt ccggctcccc gcaggcgggc tatcttcgca
      421 gccctttcaa aaaagcacgg acgtggcgga attggtagac gcgctggact taggatccag
      481 tgccgcaagg cgtgggggtt cgagtccccc cgtccgtact atcccgtggc cggggctgca
      541 tcgcaaggat gacgatctcg tcgtccatcc tcgatcccca agcgacgaga tcgtcgtcca
      601 ctcatcaagc caaggcgacg tgaacgtcgc cccatccagg atccatggcg acgtgaacgt
      661 cgccccaaca tcaatccatg gcgacgtgaa cgtcgcccca acagcatgaa tatcgaacga
      721 gaagagaccg gcaccctgac ggcaaccctg aagctgaaac tccttcccga ggactatgcg
      781 cccgccgtgg agaagaccct gaaagaacaa cgcaggacag ccgcattgcc gggtttccga
      841 cccggccagg tgcccatggc catcatcaaa aagcgcattg gccgcaacgt gctggtgaat
      901 gaggtggagc gcgtgatcga gcgcagcctg aatggataca tcaccgagaa tcgcttgcgt
      961 ccgctgggcc aaccattacc gaaaaacgag aatttctcgg cgaataactg ggatgagccg
     1021 ggcgagttca acttcgcgta cgagatgggc ctggcccccg tgttcgatgt ggagttgaat
     1081 gagaaactcg gtgtggagat gcccgtggtg gatgtggatg atgcgctggt gaacaaggaa
     1141 gtggccgaca tgcagcggcg cttcgggcaa gtggtggatg tggaaagggc cgctgacaag
     1201 gacatgttgc tgggcgacct gatcgaactg gatgaggcgg gtgccatcaa acccggtggc
     1261 ctgatgaacc gtaccaccat cagcttggag tacctcaagg acgaggaagc gcgtcggctt
     1321 ttcgttggca aggccaaggg cgatgaagtg aaggtggatc cgcaccggat cagcgatggc
     1381 cacgacgacc tggcgcgcat gctgggtgcc gatcacgagg ctgtacatgg actgcagggc
     1441 gatatgctct tccgtgtgga ggcgatcaaa cgcatggaag cggtgccgct ggatcagaca
     1501 cttttcgacc gcgtgtacgg gcaaggggcc gtgaccgacg agaccgcctg ccgggccaag
     1561 gtgaaagcgg ggctggagaa cgccttccga cgcgacagcg accgcgtgtt caagcggttg
     1621 gtgatgaaga agctccagga gaacgcccac attgagttgc ccgacgagtt cctcaaacgc
     1681 tggatccgca ccaccagcga aaagcccatc acgcccgagc aggtggagga aggctacacc
     1741 agctatgcca ccggcctgaa acgccagctg gtggaggatc gtgtggtgga gaaatacggc
     1801 ctggaggcca aaggggagga gatcgatggt ttcgccaagc ggtacatcag cgaccagttc
     1861 gtgcagtacg gcatggaagc tcccgagggt gaagccctgc aagccgccgc cgcccgcctg
     1921 ctgggcgatc gcgaccagat caagcgcatg cgcgatgcca tcgtggagca gaagctcctg
     1981 gtacatttca agaacatggt atcgcccaag gaacgtcggc ttcccttcga tgcgttcgta
     2041 aacttggccc gcaccgcctg acgcgatgcc ttgaaccaca caggaaatcc aatgatcccc
     2101 aacgacgaat tccgcaagta cgccgtgaag catcgcggca tcaacagcct ggtgctgcgc
     2161 gattaccagg cgagcatgac gccgtacatc atcgaggaac gccagctcaa cgtggcccag
     2221 atggacgtgt tcagccgcat gatgatggac cgcatcatct tcctcggctc gggcatcaac
     2281 gatcaggtgg ccaacatcgt gcaggcgcag ctcctcttct tggagagcct cgatgccaag
     2341 aaggacatcc agatctacat gaacagcccg gggggcggtg tgtacgctgg tctgggcatc
     2401 tacgacacca tgcagtacat caatcccgat gtggccacca tctgcacagg catggccgcc
     2461 agcatgggcg ccgttctgct ttgcgcggga gccaagggca agcgcagtgc gctgaagcac
     2521 gcccgcgtga tgatccacca gcccatgggt ggcgccgagg gccaggccag cgacatggag
     2581 atcacggtga acgagatcaa gaagctgaag aaggagctct acgagatcat cgccaaccac
     2641 agcggacaac cgttcgagcg tgtggagaag gatggtgatc gcgactactg gatgatcgcc
     2701 gaagaggcca aagcctacgg catgatcgac gagttgctcg ttcgccagaa gtagatctcg
     2761 acttgcagca agcgttgaag cgcgtgcgaa cgcgcttcaa cgcctttttt ggtggtgtca
     2821 agcccccgcc agggcgcctg taccacgtac agggcatcat ggacgagggg tgacccttga
     2881 ccgtatattc accgcctgtc cgggcctatt ccctgttctc ctcacctggc gcagccatcc
     2941 gatggacaaa aaagagatca agtgctcgtt ctgcgggcgc gacaagcagg ataccaacgt
     3001 cctcatcgct ggtatcaccg gacatatctg cgacagctgc atcgggcaag cgcagaacat
     3061 catccatgag gaactgagcc atcgcgaccg gaacgaaccc gacggtggca tcatcctgca
     3121 gaagccgagt gatatcaaac ggtatctgga cgaatacatc atcggacagg acgacgccaa
     3181 gaaggtgctc agcgtggcgg tgtacaacca ctacaaacgc ctgatgcaga agtccacggg
     3241 ggcggatgac gtggagatcg agaagagcaa cgtgatcctg gtgggcgaaa caggcacggg
     3301 caagaccctg ctggcacgca ccatcgccaa gatgctcaat gtgccgttcg ccatagccga
     3361 tgccaccgtg ctcaccgaag caggctacgt gggcgaggac gtcgaaagca tcctcagcag
     3421 gctgctccaa gcggccgatt acgacgtgac caaagctgag cgcggtatcg ttttcatcga
     3481 cgagatcgac aagatcagcc gcaagagcga tacaccgagc atcacacgcg acgtgagcgg
     3541 ggagggcgtg cagcaggccc tgctgaagct gctggaaggc agtacggtga atgtgccccc
     3601 gcaaggcggg cgcaagcatc ccgaacagaa gctcatccag gtggatacgc gcaagatcct
     3661 cttcgtctgt ggcggtgcgt tcgatggcat caacaagatc atcgcccgcc gcgtgaagag
     3721 cagctccgtt ggatacagcg ccagcaagga gaaccgggtt ctggatgaga acttgctgca
     3781 gtacgtggcc cccaccgacc tgcgcagcta cggcctcatt ccggagctta tcggccgttt
     3841 cccggtgctc acgtacctgg acccgctgga ccgcgaaaca ctccgtcgca tcctcaccga
     3901 accgaagaac gcgctcatca aacagtacgt gaagc
//
LOCUS       DAOQOC010000124         3916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10694_length_3916_cov_3.548822, whole genome shotgun sequence.
ACCESSION   DAOQOC010000124 DAOQOC010000000
VERSION     DAOQOC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10694_length_3916_cov_3.548822"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3916)
                     /locus_tag="PL106_03030"
     CDS             complement(<1..>3916)
                     /locus_tag="PL106_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNA32070.1"
                     /translation="VGCSGDCDATASVTVAGGTPGYTYDWSGSPVGDSTSAVTGLCPG
                     VYTVQVQDAVPCGVIQTFIIAPPVPITVDVTTQDASCAGACDGAAEAVATGGTGVYAY
                     AWWPGGQTTADVTGLCAMDHLLTVSDSSGCDTTITVSIAEPLPIDPVATVSNVGCSGA
                     CDGSIVLAPVGGSGNYTYAWSNGSSGNQVQGLCAGTVTVTVSDGACDTTLVFDITEPD
                     PLNTGLNILGAGCGGACTGTASAVHTGGTPGYDFTWGPGTIVGQGTPNASQLCPGAYT
                     LVVVDSIGCDTTISFTIEEPAAIMPAVITTLAGCGGACDGTATLDFTGGAGMVTIVWS
                     PQPGSGQGTVNATGMCPGPGIVTLTDSLGCDTTVQFIIGTPSGIVVVPTVEDASCANA
                     CDGSVELSASGGAPGYSFNWTPAVSTDSMAAGLCQGQYIVQVGDQAGCDTTVVVDVGA
                     PPPISITGSFTNESCFGPCDGTASVTAAGGSGPIGFTWSPTPGTDDGNGNVTGLCAGE
                     WTVTATDAAGCDTSWTFTLLPQQPIAAELAVEDGTCAGTCSGTASVNVTGGSGTPTYD
                     WAPGTPAGDGTNAVTGLCLGTHSVTITDAAGCDTTIAFVINIPPAIAPNLSVSAADCS
                     GPCSAEAVSATAGGTPGYTWTWQPEPGGGQGTASVTGLCADTTYSLIITDAQACETVV
                     NFTVPGYTPLSTATTTTFVNCYGTCDGNATLNTTGGTGPYTFDWTPDPQGGDGNDAAT
                     GLCADFYSVLITDANGCDTTVSLPIGTNGPITVQPVITPIACNGDCNGSIQLNATGGS
                     GVLTYLWGPSPTGQGTDIASQLCAGQWTVTITDGLGCDTTFVFSLAEPPPMVIQAEVS
                     ASQCQACDGSITMHASGGGGGYFFAWGPPINVTTSDSVQSGLCAGIYTVIVGDALGCV
                     TQLAVPVSDSNTEVLTVTDGSVTCPTDCNGAVSVALNCSVPSCTIAWFDGIGTNLNVS
                     TNTMNGLCAGNYLVQVTNGDGCLNIDTAVVVAPDPIVATLGLTSPGCADMCDGTSTGT
                     VTGGQGPWTVTWDPAPLTGQGSTDVTGLCATTYQLTIEDDQGCTVVQSVNVPGPSPIQ
                     TNATVQDITCAGACDGGIALNATGGTGTLGYSWSPGGQSTPTVTQLCAGDWSVTIIDA
                     NGCDTAFTFTLTEPVPLALTTTATPSQCLVCAGTVSVDVSGGTGTPTIVWTDMNGGTV
                     GTGSTVTNLCAGIYTATVQDVNGCSTQAIAVVGDATGEMITVTDGVTSCPDVCDGTVS
                     VSFACVNGPCSILWADMNGQLLAQNQFGIGGLCADNYLVQVVN"
BASE COUNT          708 a         1286 c         1189 g          733 t
ORIGIN      
        1 gttcaccacc tgcaccaggt agttgtccgc acacaatcca ccgataccga actggttctg
       61 ggcaagcagt tggccgttca tatccgccca gaggatggaa catggaccgt tcacgcacgc
      121 gaacgatacg gacaccgtcc catcgcaaac gtccgggcaa ctcgtcacgc catcggtcac
      181 cgtgatcatt tcaccggtcg catcgccgac cacggcgata gcctgggtcg aacaaccgtt
      241 cacatcctgc acagtggccg tatagatacc ggcgcagagg ttcgtgaccg ttgatcccgt
      301 tcccaccgtc cctccgttca tgtccgtcca aacgatagtg ggcgtaccgg tgccgccgct
      361 cacatccacg ctcaccgtac cggcacaaac caggcattgg ctgggtgtcg ctgtcgtggt
      421 cagcgcgagt gggaccggtt cggtcaaggt gaaggtgaac gctgtatcac aaccgttcgc
      481 atcgatgatg gtgacggacc agtcaccagc gcacaattgt gtgaccgtag gggtgctttg
      541 accacccggg gaccacgaat accccaacgt ccccgttcct ccagtagcat tcagtgcgat
      601 accgccatcg catgcaccgg cgcatgtgat gtcctgcacc gttgcgttcg tttggatcgg
      661 ggacggtccg ggaacgttca cgctctgtac cacggtgcag ccttgatcgt cctcgatggt
      721 cagttggtaa gtggtcgcgc aaaggccggt cacgtccgtg gaaccctgtc ctgtcaaggg
      781 tgcgggatcc catgtcaccg tccatggacc ctgccccccg gtgaccgttc cggtcgaggt
      841 tccatcgcac atgtccgcac aacccgggga tgtcaggcca agtgtggcca ctatgggatc
      901 cggggccacc accacggccg tatcgatgtt caagcagcca tcgccattgg tcacctgtac
      961 caggtagttc ccggcgcaca gtccgttcat cgtgttcgtg ctcacattca agttggtccc
     1021 gatcccatcg aaccaggcga tcgtgcatga cggcacggaa cagttgagag ccacgcttac
     1081 cgccccgttg caatcggtgg ggcaggtcac gctgccgtcc gtgacggtca gcacttccgt
     1141 gttgctgtcg ctcacgggta cggcgagctg cgttacgcag cccagcgcat caccaacgat
     1201 caccgtatag atcccagcgc acaggccgct ctgcacgctg tccgacgtgg tcacgtttat
     1261 cggtgggccc caggcgaaga agtatccgcc gcccccgccg gaagcatgca tggtgatgga
     1321 gccgtcgcag gcctggcatt gactggccga gacttcggcc tgtatcacca tgggtggcgg
     1381 ctcagcgagg ctgaatacga acgtggtgtc gcaccccagg ccatcggtaa tggtcaccgt
     1441 ccactgtcca gcacagagct gcgaggcgat atccgtaccc tggcccgtgg gtgaagggcc
     1501 ccataaatag gtcagcactc ccgatcctcc agtagcgttc aattggatgc taccgttgca
     1561 atccccgttg cacgcgatgg gtgtgatgac cggttgcacc gtgatcgggc cgttcgttcc
     1621 gatgggcagg ctcaccgtgg tatcgcagcc gttcgcatcc gtgatcagca ccgaatagaa
     1681 gtcggcgcaa aggccggtgg ccgcatcatt cccatctcca ccttgcgggt ccggggtcca
     1741 gtcgaacgtg tacggtcccg tgccgccggt cgtgttcagc gtcgcgttcc cgtcgcaggt
     1801 accatagcaa ttgacgaatg ttgtcgtggt ggccgtactg aggggtgtgt agcccggcac
     1861 ggtgaaattc accacggtct cacacgcctg cgcatcggta atgatgagcg aataggtcgt
     1921 gtcggcgcac agtccggtaa cggatgctgt accctggcct cctccgggtt cgggttgcca
     1981 ggtccaggtg tagcccggtg tgccacccgc cgtggcggag acggcctcgg ccgaacatgg
     2041 tccggagcag tcggctgcgg acacgctcaa gttgggcgca atggccggag ggatattgat
     2101 cacgaacgcg atggtcgtat cacaaccggc cgcgtcggtg atggtcacgc tatgcgtacc
     2161 gagacacagg ccggtcaccg cattcgtacc atcaccggcc ggcgtacccg gcgcccagtc
     2221 gtacgtgggc gttcccgatc caccggtaac gttcacgctc gcggtgccgc tgcacgtgcc
     2281 agcgcacgtg ccatcctcca cggccaactc cgctgcgatg ggttgctgcg gcagcagggt
     2341 gaaggtccac gatgtgtcgc atcctgcggc gtccgtcgcg gtcacggtcc attcgcccgc
     2401 gcacaggccg gtcacgttgc cgttgccatc atcggtaccg ggcgtggggc tccaagtaaa
     2461 accgatcgga ccgcttccgc cggcggcggt cacgctcgcg gtcccatcac aaggtccgaa
     2521 gcagctctcg ttggtgaagc tgccggtgat gctgatcggt ggtggtgcgc caacatctac
     2581 taccacagta gtatcacaac cggcctgatc gcccacttga acgatgtact gtccttgaca
     2641 cagtccggcg gccatggagt ccgtcgaaac ggccggcgtc caattgaagc tgtatccggg
     2701 ggcaccaccg gaagcggaca gttccaccga cccgtcgcag gcgttcgcgc atgaggcatc
     2761 ttccacggtc ggcaccacta cgataccgct gggagtgccg atgatgaact ggaccgtggt
     2821 gtcgcaaccc aggctgtccg tcagggtgac gatacccgga cccgggcaca tgcccgttgc
     2881 attcaccgtg ccctgtcctg agccgggttg tgggctccag acgatggtga ccatgcccgc
     2941 accgcccgtg aagtccagcg ttgcggtccc gtcgcatgct ccaccgcagc ccgccaacgt
     3001 ggtgatcacc gcgggcatga tcgccgccgg ctcctcaatg gtgaaactga tggtggtgtc
     3061 gcagccgatg ctgtcaacca caacgagggt gtatgctcct gggcagagct ggctcgcgtt
     3121 cggagtgccc tgaccgacga tggttccagg cccccacgtg aagtcatagc ccggggtccc
     3181 accggtatgc acggcgctcg ctgtacccgt gcaggcacct ccgcaacccg cgcccaggat
     3241 gttcaatccc gtgttcaagg gatcgggctc cgtaatgtcg aacaccaggg tggtgtcgca
     3301 ggcgccatcg gacacggtga cggtgaccgt gcccgcgcac aggccctgca cctggttccc
     3361 ggacgatcca ttggaccatg cgtacgtgta gttgcccgaa ccgccgaccg gagccagtac
     3421 gatgcttcca tcacaagcac cgctgcaacc cacgttggat accgtggcca caggatcgat
     3481 cggcagcggt tcggcgatgc tgacggtgat cgtggtatcg cagccggagg agtcgctcac
     3541 ggtaagcaga tggtccattg cgcacaagcc cgtaacgtcg gctgtcgttt gcccgccagg
     3601 ccaccaggcg taggcgtaca ccccggtgcc ccccgttgcc accgcctcgg ccgcgccatc
     3661 acaggcaccg gcgcaactgg cgtcctgtgt ggtcacatcc acggtgatcg gcacgggtgg
     3721 tgcgatgatg aaggtctgga tcactccaca aggcaccgca tcctgcacct gtaccgtata
     3781 cacccctggg cagagccccg ttaccgcgga tgtactgtcc cccaccgggg agcccgacca
     3841 gtcataggta taacccgggg tgccacccgc aacggtgacg ctcgcggtcg catcgcaatc
     3901 gccgctgcaa cccaca
//
LOCUS       DAOQOC010000125         3898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10773_length_3898_cov_4.284153, whole genome shotgun sequence.
ACCESSION   DAOQOC010000125 DAOQOC010000000
VERSION     DAOQOC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10773_length_3898_cov_4.284153"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PL106_03035"
     CDS             complement(<1..615)
                     /locus_tag="PL106_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNA32071.1"
                     /translation="MGSDLMYYIPALGVVGLVVMVFKAMWVSKQDAGDANMQELAGYI
                     AKGASAFLKAEWKVLGVFAVIAAVLLAWSGTLVEHSDWVIAVAFLIGAFFSAFAGWIG
                     MSIATKANVRTTQAARTSLAKGLQVSFNGGTVMGLGVSGLAVLGLSVLFIAFYKMYVN
                     GAHPNGEEMMKALEVLAGFSLGAESIALFARVGGGIYTKAADVGA"
     gene            820..2469
                     /locus_tag="PL106_03040"
     CDS             820..2469
                     /locus_tag="PL106_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32072.1"
                     /translation="MKQRYTALLLIPLFVPFGQAQTPFEYADQRTAYVHYDDVIPFGT
                     GGWLTWGGACDDSGVNCGNAVVRYDGAGTLLSTVGVPVGSWSDLATMPDGGVLICGSS
                     PMCDVIVNTSAAVRLDATGSILWTREFVLADLRHCTVATSGTIAMATEDTVLINTADG
                     DSLRAWPSNTGTIAEMHWLNDSSLLVAGASAIAIMDPMGTTILSIANTQEIRDVTVVN
                     DEVLLLLPGMIQSRSDQLLPIDSIDLSAWTSDAKGFTGDGTTNWVRSSTGMIQFELDQ
                     GVLNNFDFDAPEGYQVLGAAVQGATISTCGVGTYLGRTSGVVRTHLFDGASAEHDNNV
                     GIFIASVDSTWTNSPWTNVYAFHQLCHVGVVNHGTTPVSELMIEYFGTSWICGPAGVV
                     MDLTGLQLAPGDTAVFPFPELLTTYFTIPQNDSVEVEFCLVALSPDNIFDRDLSDNET
                     CSVVQFVNTVGMDEEEMGAIRVGPNPISDRIDITFASPLRGALNAQLFDPTGRQLAEV
                     AVPAGSSTFSWPVLVGQGGVYVLTLSGQDLFRSLKLVASDR"
     gene            complement(2477..3496)
                     /locus_tag="PL106_03045"
     CDS             complement(2477..3496)
                     /locus_tag="PL106_03045"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNA32073.1"
                     /translation="MLLSPQAHQPFHMRTIQFREALNEAMSEEMRRDPNVFLLGEEVA
                     EYDGAYKVSKGMLAEFGEKRVIDTPIAELGFTGIAVGAAMNGLRPIVEFMTWNFAVLA
                     SDQIINHAAKMLQMSGGQFHVPIVFRGGNGSAGQLAATHSQSFEAMYANVPGLKVISP
                     STPYDAKGLLKAAIRDNDPVLFMESERMYGDKGEVPDGEYLLPIGLADVKREGKDVTI
                     VSFNKMMKVALGAAVELAKEGISAEVIDLRTIRPLDHVTIIRSVQKTNRLVVVDENWP
                     FASIASEVAYRVQKDAFDYLDAPVFRVNQADTPLPFAPGLIEESLPSVARVVKAVKEV
                     MYVVK"
     gene            complement(3613..>3898)
                     /locus_tag="PL106_03050"
     CDS             complement(3613..>3898)
                     /locus_tag="PL106_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNA32074.1"
                     /translation="FESRLFFTDKLIEMGAQITLCDPHRALVIGKDFEKPLRAIRMTS
                     PDIRAGVSLLIAALSADGTSTIDHIEQIRRGYQNIEGRLNAIGARISVLD"
BASE COUNT          715 a         1207 c         1167 g          809 t
ORIGIN      
        1 ggcgcccacg tcggcggcct tggtgtagat gccaccgcca acacgagcga acaacgcgat
       61 ggattcagcg ccgagcgaga agcccgccag cacctccaac gccttcatca tctcttcacc
      121 gttcgggtgg gcgccattca cgtacatctt gtagaacgcg atgaacagca cgctgaggcc
      181 cagcaccgcc agaccgctca cgccgaggcc catcacggta ccgccattga agctcacctg
      241 caggcccttg gccaggctcg tgcgcgccgc ttgcgtggtg cgcacgttgg ccttggtggc
      301 gatgctcatg ccgatccaac cggcgaaggc gctgaagaag gctccgatca ggaaggccac
      361 cgcgatcacc caatcactgt gctccaccaa cgttccgctc caggccagca gcaccgccgc
      421 gatcacggcg aacacgccca gcactttcca ttcggccttc aggaaggcac tggccccctt
      481 ggcgatgtag cccgccagct cctgcatgtt ggcatcgccc gcatcctgct tgctcaccca
      541 catcgccttg aacaccatca ccaccaatcc caccacgccg agcgcgggga tgtagtacat
      601 caagtcactt cccatgttac tgctacgtta ggttatctcg ttgtccctca gcggttacgt
      661 tccggcaaag ggggcgcgaa aataggattc cttggaaatg tgggcttgtg tcttacgcat
      721 gcacaagtcg caattgggga gctccggggg cgtcgtacat caggacaggc agcgatcgta
      781 tggtcctctg gcgtccagtg tatactctac cttcaaccca tgaagcagcg atacacggca
      841 ctccttctga tccccttgtt cgtgcctttt ggccaggcac aaacgccctt cgagtatgcc
      901 gaccagcgca ccgcttacgt gcattatgac gacgtgatcc ctttcggtac cggggggtgg
      961 ttgacatggg gcggggcttg cgatgactcg ggagtgaact gcgggaatgc tgtggtccgg
     1021 tacgatggtg ccggaaccct gctttccaca gtgggtgtcc ctgttggttc ctggagtgat
     1081 ctggcgacga tgccggatgg tggggtgttg atctgcgggt cttccccgat gtgcgacgtt
     1141 attgtgaaca ccagcgccgc cgtgcgtttg gacgcgaccg gctccatatt atggacacgc
     1201 gagttcgtcc ttgcggactt gcggcattgt accgtcgcca cctcgggcac gatcgcgatg
     1261 gccacagagg acacggtact gatcaacacg gccgacggcg acagcttgcg tgcttggcct
     1321 tccaatacgg gaaccatcgc agaaatgcac tggctcaacg acagttcgct cctcgtggca
     1381 ggcgcctccg cgatcgccat catggacccg atgggaacga ccattctgag cattgccaac
     1441 acgcaggaga tccgcgacgt gaccgttgtg aacgacgaag tgcttctgct cttgcccggt
     1501 atgatccaga gccgtagcga tcaactcctg ccgatagact ccatcgacct gtccgcttgg
     1561 acatctgatg ccaagggttt cactggtgat ggaaccacga actgggtgcg ctcaagcacg
     1621 ggcatgatcc agttcgaatt ggaccagggt gttttgaaca acttcgactt cgatgcgccc
     1681 gaaggctatc aggttcttgg ggctgcggta cagggtgcaa cgatctccac ttgtggcgtc
     1741 ggtacctatc tcggcaggac cagcggcgtt gtgcgcaccc acctgttcga tggtgcgagc
     1801 gccgagcacg ataacaatgt gggcatcttc atcgcgtcag tggattccac atggaccaat
     1861 tcaccttgga ccaatgtgta tgctttccac cagctctgcc acgtgggcgt ggtcaaccac
     1921 ggcactacac ccgtgagcga attgatgatc gaatatttcg gaacctcttg gatctgcgga
     1981 cccgccgggg tggtcatgga tcttacgggc ctgcaactgg cgccgggcga tacagctgtc
     2041 ttccccttcc ccgaactgtt gacaacgtac ttcacgatac ctcagaacga cagcgtggag
     2101 gttgaattct gccttgtggc tttgtcaccg gacaacatct tcgaccgcga cctgtccgac
     2161 aacgagactt gtagcgtcgt tcagttcgtg aacacggtag gtatggacga agaggagatg
     2221 ggcgcgatcc gtgtcggtcc aaacccgatc agtgatcgca tcgacatcac cttcgcttca
     2281 cccctgcgtg gggcactgaa cgcacagctg ttcgatccca ccgggcggca acttgcggag
     2341 gtggccgttc cggcgggaag ctcaacgttc tcgtggcccg ttctggtcgg tcagggcggt
     2401 gtgtacgtgc ttactctgag tggacaggat ctattccgct cgctgaagct ggttgcttcg
     2461 gacaggtgag tgcattctac ttcaccacgt acatcacctc cttcacggcc ttcaccacac
     2521 gtgccacgct gggcagcgac tcttcaataa ggcccggggc gaaaggcagg ggcgtgtcgg
     2581 cttggttcac acggaacacg ggggcgtcca agtagtcgaa cgcgtccttc tgcacccgat
     2641 aggccacctc gctggcgatg ctggcgaagg gccagttctc gtccaccacc accaggcggt
     2701 tggtcttttg tacgctgcgg atgatggtaa cgtggtccaa cggacggatc gtgcgcaggt
     2761 cgatcacctc ggcactgatg ccctccttgg ccagttccac tgccgcgccc agggccacct
     2821 tcatcatctt gttgaaggaa acgatggtga cgtccttccc ttcgcgcttc acgtcggcca
     2881 gaccgatggg cagcaggtac tccccatcgg gcacctcgcc cttgtcgccg tacatgcgct
     2941 cgctctccat gaagagcacc gggtcgttgt cgcggatggc ggctttcagc aggcccttcg
     3001 catcgtacgg ggtgctgggg ctgatcacct tgaggccggg cacgttggcg tacatggctt
     3061 cgaagctctg gctgtgcgtg gcggccaatt gccccgcgct gccgttgccc ccgcggaata
     3121 cgatgggcac gtggaactgc ccgccgctca tctgcaacat cttggccgcg tggttgatga
     3181 tctggtcgct ggccagcaca gcgaagttcc aggtcatgaa ttccacgatg ggccgcagcc
     3241 cgttcatggc ggcacccacg gcgatgccgg tgaaacccaa ctcggcgatc ggcgtgtcga
     3301 tcacgcgctt ctcaccgaat tccgccagca tgcccttgct caccttgtac gccccatcgt
     3361 actcggccac ctcctcgccc aggaggaaga cgttcgggtc gcggcgcatc tcttcggaca
     3421 tcgcctcgtt cagtgcttcg cggaattgga tggtgcgcat gtggaagggc tggtgggcct
     3481 gcggcgaaag taacaggtaa caaggaaggg ctgcttgtgc cgggctccgg aaaaccggcc
     3541 ggaaccttcg ctacgatgct gcttctccag gaacaccacc ggccatccgt agcgcatggc
     3601 cggtgggagg gatcagtcga gcacgctgat cctggcgcca atggcgttca ggcgcccttc
     3661 gatgttctgg tagcccctgc ggatctgctc gatgtggtcg atggtgctgg tgccgtcggc
     3721 gctgagggcc gcgatgagca gggagacgcc cgcgcggatg tcgggactgg tcatgcggat
     3781 cgcgcgcaac ggcttctcga aatccttgcc gatcaccaag gcgcggtgcg ggtcgcagag
     3841 cgtgatctgc gcacccatct cgatgagttt gtcggtgaag aacaggcggc tctcgaac
//
LOCUS       DAOQOC010000126         3891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10806_length_3891_cov_3.306830, whole genome shotgun sequence.
ACCESSION   DAOQOC010000126 DAOQOC010000000
VERSION     DAOQOC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10806_length_3891_cov_3.306830"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PL106_03055"
     CDS             <1..1580
                     /locus_tag="PL106_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA32075.1"
                     /translation="GVAMRLPAETTSAMALVNDVVQDREGHFLYATSGGIIPWAVTDP
                     LVSAGFVGGTDRVILAPGEDVRQMLANDGQLWAITRSGTLLSILDTVVRRFVPPVHVR
                     SGSLGATADGDGNLWITTDDGLLRFNPDDSSFLHVPVGDGSGVRKLSRAITTLADGRI
                     AFCADNAVFTFDPEAYAVPPPIPVPYVRGATVAGSPVEFAQGGVELSYRASVIDINIS
                     ALALGFAIAPVFEYRLEGVEHDWRETPAREAIRYAGIPVGRHRLLVRVRDPFGRTGPV
                     HELLLISVRGPIWQQWWFYVIAVLTVSAGLYALYIFRLRQAMKLQAVRNRIASDLHDE
                     VGSSLSSITIGAQLAASLSPEASEQVKMLLSRMGETSSASLRSISDIVWAIDPKNDEG
                     EALVKRMRRIAQELLESNDVDVSFDIGPGVEELKLPMNARKEIVLIYKEAVHNASKYS
                     GASFVLVSLHRRNGSLAVSVKDDGKGFDVALHPDGHGLGSMRRRADAIGAELTLTSAP
                     GMGTLVGVEVDLTRIRD"
     gene            1623..3479
                     /locus_tag="PL106_03060"
     CDS             1623..3479
                     /locus_tag="PL106_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32076.1"
                     /translation="MRKTVPLRLIFSLFPISSFAQQTEIEPNEDFTASTNWTYGQTMS
                     GTECLPPASDADHFRIVLPTDGVINFTVTTQGSGTQPGTLQFALFPKYGIYQQYHTLA
                     GDLLGTTVSFSHSCLSGDTMYVRMNQADAGNNSCISYEMSCTVTPTVFANDVLPNDDF
                     ANPQPVALGTPIEGHMNFLYDNTSDHYGVTLPSDGVLRIIAEAEHADTATDGAVQLYV
                     NVDNSYHYPAIGANGVVATDTFYLSCFAEQLVTIRVQNSNANTCGVSYRLRFDHTPHL
                     FASDPLPNDDFANPQPIALNTPIEGHLDHYGETTADHYGLTLPDDGTLLIIAEAEQYG
                     PDTTGAVEVYVNMNNSYAYVPIGANATAGLDTFRVECLRAGNVTVRLQPNTPEGCGIS
                     YRVRFELIPSAFGNDPEPNEGAADATVVAPDTDQDGHLAYIGTSTMDHYKLWKGFAGT
                     MRVIISSTTEGPSPAINLWTLNANVNENVSTGENGDIVTDTVLVTTANPDTILLRVSA
                     VDFSYCGSYHFHYESGPMGLGAQLGGGPMTQVFPNPSISGRFTVHHSLPGPIMIAVQD
                     AQGSVIYRSRAANGPLDLDLSHLPSGLYVTHLMADQGSASNVRLMISGPENR"
     gene            3491..>3891
                     /locus_tag="PL106_03065"
     CDS             3491..>3891
                     /locus_tag="PL106_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA32077.1"
                     /translation="MADRVRVIAFDDNKDLREMFRMLVDAQADMVCVVVLPDLSQLMR
                     DIDAAQPDVIVMDIQMPGMNGIDGVKAIKTRYPEARILMQTVFEDEDKVFDAICAGAS
                     GYILKTASADEMVGAIRSVHAGGSPMTPSIAA"
BASE COUNT          766 a         1194 c         1191 g          740 t
ORIGIN      
        1 tgggcgtggc catgcgcctg ccagcggaaa ccacctcggc catggcgctg gtgaacgacg
       61 tggtccagga ccgtgaaggg catttcttgt acgcgacatc aggcggtatc atcccatggg
      121 cggtcaccga tccgttggtg tccgccgggt tcgtgggcgg gactgaccgc gtgatcctcg
      181 cgccggggga ggacgtacgg cagatgctgg cgaacgatgg ccagctgtgg gccatcaccc
      241 gttcgggcac gctcctgtcg atcttggata cggtggtccg gcgcttcgtt cccccggttc
      301 acgtgcgttc cggatcgctc ggggccacgg cggatggcga cgggaacctg tggatcacca
      361 cggacgatgg actgctgcgc ttcaacccgg atgacagttc gttcctccat gtgcccgtgg
      421 gcgacggatc gggcgtgcgg aagctctccc gggcgatcac cacccttgcc gatggccgga
      481 tcgccttctg tgccgacaat gcggtgttca ccttcgatcc cgaggcctac gctgttccgc
      541 ctccgatccc cgtgccttac gtgcgcggtg caacagtggc aggtagtccc gtggagttcg
      601 ctcagggggg cgttgaactg tcgtaccggg ccagtgtgat cgatatcaac atcagtgcac
      661 tggcactcgg gttcgccatt gcgcccgtgt tcgagtaccg gcttgagggg gtggagcacg
      721 actggcgtga aactccggcc cgtgaagcca tccgctatgc gggcataccg gtgggcaggc
      781 accgattgct cgttcgtgtt cgggatccgt tcgggcgtac gggtccggtg catgaactgc
      841 tgctgatcag cgtacgggga cccatctggc aacagtggtg gttctatgtg atcgccgtgc
      901 tcacggtgag cgcggggctg tatgcattgt acatcttccg tctacgacag gccatgaagt
      961 tgcaggccgt gcgcaaccgc atcgccagcg acctgcatga tgaggtcggc tcttcgctca
     1021 gttccatcac catcggcgcc cagctggccg ccagcctttc gccggaagcc agcgagcagg
     1081 tcaagatgct gctttcacgc atgggcgaaa caagcagcgc cagtttgcgc agcatcagcg
     1141 atatcgtgtg ggccatcgac ccgaagaacg atgaagggga ggccttggtg aagcgcatgc
     1201 ggaggatcgc gcaggaattg ctggagagca atgacgtgga cgtgtccttc gatatcggcc
     1261 ctggtgtgga ggagctgaag ctgccgatga acgcccgcaa ggagatcgta ctcatctaca
     1321 aggaggccgt gcataacgcg agcaagtaca gcggcgcttc gttcgttctg gtttcgctcc
     1381 atcgccggaa tggatccctc gcggtgagcg tgaaggatga cggcaaggga ttcgatgtcg
     1441 cgctccaccc cgatggccac ggccttggaa gcatgaggcg gagggccgat gcgatcggtg
     1501 ccgaactcac cctcaccagc gctcccggca tgggtacgct cgtgggagtg gaggtggacc
     1561 tcacgaggat ccgtgattga tggccggcgc gtttcgtcgt cactttatgc ccaaaccgac
     1621 ccatgcgcaa aaccgtaccc ttgcgtctga tcttttcctt gttcccgatc agttcgttcg
     1681 cccaacagac cgagatcgaa cccaatgagg acttcaccgc gtccaccaat tggacctatg
     1741 gccagacgat gagcggcacc gagtgcttgc cgccggcgtc cgatgcggat cacttccgga
     1801 tcgtactgcc gactgacggg gtcatcaatt tcaccgtgac cacccaggga agcggaaccc
     1861 aacccggtac cttgcaattc gcattgttcc ccaagtacgg gatctatcag cagtaccaca
     1921 cgctagcggg cgacctgttg gggaccacgg tatccttcag ccacagctgc ctcagcggcg
     1981 atacgatgta cgtgcgcatg aaccaggccg acgcggggaa caactcctgc atcagttatg
     2041 agatgagctg caccgtgacg cccaccgtgt tcgccaacga tgtgctgccc aacgatgact
     2101 tcgccaaccc acaacccgtg gctttgggca cgcctatcga aggccacatg aacttcctct
     2161 acgacaatac ttcggaccat tatggggtga ccttgcccag cgatggcgtg ttgcgcatca
     2221 tcgccgaggc ggaacacgct gatacggcga ccgatggtgc cgtacaactc tatgtgaacg
     2281 tcgacaacag ctaccactat cccgccatcg gtgcgaacgg cgtggtggcc acggacacgt
     2341 tctacctttc ctgcttcgcc gagcaactcg ttacgatccg ggtgcagaac agcaacgcga
     2401 acacatgcgg agtaagctat cggctccgct tcgatcatac cccccacttg ttcgcgagcg
     2461 atccgctgcc caatgacgac ttcgcgaacc cgcaacccat cgcgctcaac acaccgatcg
     2521 aagggcatct ggaccactat ggtgagacga ccgccgacca ctacggcctc acgctcccgg
     2581 acgacgggac actgttgatc atcgcagagg ccgagcagta cggcccggac accacgggcg
     2641 ccgtggaggt gtacgtcaac atgaacaaca gctacgctta cgtcccgatc ggtgcgaatg
     2701 ccactgcggg gctggatacc ttccgtgtgg agtgcctgcg tgctggaaac gtcaccgtgc
     2761 gcctacaacc gaatactcca gaaggttgcg ggatcagcta tcgggtgcgt ttcgaactga
     2821 taccgtcggc gttcggcaac gaccccgaac cgaacgaagg cgcggcggat gccaccgtgg
     2881 ttgcaccgga taccgaccag gacgggcatc tggcctatat cggcacgtcc accatggacc
     2941 actacaagct gtggaaggga ttcgccggca cgatgcgcgt gatcatttcc tccacgaccg
     3001 aagggccttc ccccgcgatc aacctgtgga ccctgaacgc caacgtgaac gagaacgtga
     3061 gcacaggtga gaacggcgac atcgtcaccg acaccgtgct tgtgacaacg gcgaacccgg
     3121 acaccatcct gctgcgtgtg agcgcggtcg acttcagtta ttgtggctcg taccacttcc
     3181 actatgagag cgggcctatg ggcctgggcg cgcagcttgg aggtgggccg atgacgcagg
     3241 tattcccgaa cccgagcatc agtggccgct tcacggtgca tcattcactg cccggaccga
     3301 tcatgatcgc ggtccaggac gcacagggtt cggtcatata tcgctccaga gctgccaatg
     3361 gaccactgga ccttgacctg tcgcatctgc catcaggtct ctacgtgacc catttgatgg
     3421 ccgaccaggg gtccgcatcg aatgtgcgcc tgatgatatc aggccccgaa aaccggtaac
     3481 attacatgcc atggccgacc gtgtccgcgt catcgccttc gacgacaaca aggacctccg
     3541 cgagatgttc cgcatgctcg tggacgccca ggccgatatg gtctgtgtgg tggtgcttcc
     3601 cgacctgtcg caattgatgc gcgacatcga cgctgcgcag cccgatgtga tcgtgatgga
     3661 catccagatg cccggcatga acgggatcga tggggtaaaa gccatcaaaa caaggtatcc
     3721 ggaggcgcgc atcctgatgc agacggtgtt cgaggatgag gacaaggtgt tcgacgcgat
     3781 ctgtgccggt gcgagcggat acatcctgaa gacggccagc gcggatgaaa tggtgggcgc
     3841 catccgctcc gtacatgcgg gtggatcacc gatgacaccg agcatcgccg c
//
LOCUS       DAOQOC010000127         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10827_length_3887_cov_4.382046, whole genome shotgun sequence.
ACCESSION   DAOQOC010000127 DAOQOC010000000
VERSION     DAOQOC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10827_length_3887_cov_4.382046"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..>3887
                     /locus_tag="PL106_03070"
     CDS             49..>3887
                     /locus_tag="PL106_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32078.1"
                     /translation="MFVNVYNVEVLQSNPDIAFIATTNGLYKTTNALATASSVTWELV
                     FAPTDENYWSGLTFGKGSATVLYASGKNVYRSTDQGDTWSTITGPGTGLDLSDATWSA
                     RPAVSRIHVEAARSSAYSNYLYALVNTGRNNSGAGSYPFRFDASTGTWEGSTTALSSG
                     GATAPGWVPIGISPQDANGHLVYYGGLQIKRSPNHWQSPYTAIATTKVHDDVQALVFT
                     PSGDELWAGTHGGVFRCQDAYATAITNAPWQARNSGLNVGLVYCNASSPTDRFGVLSG
                     DQDNGLRRMDPGLGEPGTWRSLLIGDGSRVAYSSDGEHEYGVDYRNMTVYKWRDHSTT
                     TNESTNLAAIGAWTGDPRRAIEPNENDGRMMFGYTDIWEETDPDATIGNMGSSGGHIR
                     LTQYEFDNIDEYIVDCMRMQFTDAVIAPTNEAYIYLSTPARAQNSDCPNGPYPSSLIR
                     SIIPAQINMFGHYALEGVELPGAGISVTQIVVDPTDPERIWIACSGFEADQKVLCSNS
                     RGDVGSWVNWDPNGTLPNLPVNDLIYQKGTDGGLYVAMDVGVYYRNNSSEDWTPYYET
                     LPNVQVNDLDINYCAGKIRAGTYGRGLWESDLAERPTVEKVVSASVTWDFHRNLAQDL
                     HVTNNATLTISSTVNFAPDTRLIIDPGAHVIVLDGGRLTNLCGEDWDRVEVRGNTSVD
                     QVPLTDQGYLELRAGAVVEHVRNGVCLFGTDAEGDPDLATTGGVVRSIGTATNPVIFR
                     DCKRGIWAKDYNFYQRSKVLYTNMESSLDGTIDHIRLEGLRNFPINHSSFTAVGFHDT
                     PAPTGIRSFDSRFTVQDCSFANLTTGVRASDATLVKPCYVRNNSFDHCDRGITLNGVY
                     NAEVTGNTFLIPASGGSWIPDPSMFYYPIPYGAYLYDCEGYEVEGNDFMGTSAHGNIG
                     LLIRGSDQLPNEFALNTFKKLYVGSQVQGYNRSPDDGLKFRCNLYGNTDGTDGCVYDM
                     ALTTDDAAIAPVQGSVDDPAGNRFYPNCTTSGEETDIYVDHQSSSGFLYFHHEDEFTD
                     PGCITSAVIPQATDYGFDESGPGTSCPDDPSHVISISAHKSAFHAATGPYVALKAVYD
                     GEVNGGNTEDLVHMVADENIPSFTLRNALLAASPRLNDSVVIPAILRDPAMDEGHLVQ
                     VLLANSPLSADAGQAVKGSYVEELYTELWEQQQNHELRWTTLVEAELSLLRIGAERSR
                     HDYFRGLLENDSTGIALMSMDEVVNDTVVPATPMEQIALLIAQADWTEADDRLADPNA
                     YGLREDQAVVMTVVRLIT"
BASE COUNT          861 a         1145 c         1114 g          767 t
ORIGIN      
        1 aataagttcg tttttggcaa ggagcaaaat ctgctttccg aagccacgat gttcgtgaat
       61 gtgtacaacg tggaggtgct tcagtccaac ccggatatcg ccttcattgc aactacgaat
      121 ggactataca agaccaccaa cgcgcttgcc actgcgagtt ctgtcacttg ggaactcgtt
      181 ttcgcgccca cagatgagaa ctattggagt ggattgacct tcggaaaagg gagtgcaacg
      241 gttctttatg catccggcaa gaatgtgtac cggtccacag accaagggga tacgtggagc
      301 acgatcacgg ggccgggcac ggggctggat ctgtctgatg ccacttggtc ggcacgtcca
      361 gcagtatccc ggatccacgt tgaggcggct cgcagttcag cctattccaa ctatctgtac
      421 gccttggtca acacgggccg gaataacagc ggcgctggga gctacccctt ccgcttcgat
      481 gcgagtacgg gcacttggga aggaagtacc actgcactca gcagcggggg cgcgaccgca
      541 ccgggatggg tgcccatcgg catttctccg caggatgcca acggccattt agtatactat
      601 ggtggattgc agatcaaacg ttcgccgaac cactggcaat cgccctatac cgccattgcc
      661 accacaaagg ttcatgatga cgtgcaggca ctcgttttca ccccgagtgg cgatgagctc
      721 tgggctggta cacacggggg cgtgttccga tgccaagacg catacgcgac ggcgatcacc
      781 aatgcccctt ggcaagcaag gaattccggt ctgaatgtgg ggctggtcta ttgcaacgcc
      841 agtagtccga ccgatcgttt cggtgtgcta tccggtgacc aggacaacgg cctgcgtcgc
      901 atggatcctg gcctgggtga accgggcaca tggcgctcgc ttttgatcgg cgatggctcc
      961 cgcgtggctt acagttcgga cggcgagcac gagtacggcg tggactacag gaacatgact
     1021 gtgtacaagt ggcgggacca cagcacgaca acgaacgagt ccaccaatct ggccgcgatc
     1081 ggggcatgga ccggagatcc aagaagagcg atcgagccca acgagaacga cgggcgtatg
     1141 atgttcggtt atacggacat ctgggaagaa accgatccgg acgcaacgat cggaaatatg
     1201 ggttcctccg gcgggcacat ccgtctgacg caatacgagt tcgacaatat cgacgaatac
     1261 atcgtggatt gcatgcgcat gcaattcact gacgcggtga tcgccccaac gaacgaagct
     1321 tacatctacc tgtcgacccc ggctcgtgca caaaacagcg attgcccgaa cggcccgtac
     1381 ccctcatcgt tgatacggtc gattatcccg gcgcagatca acatgttcgg ccattatgcg
     1441 ctggagggtg ttgaactccc cggtgctggc atctcagtca cgcagattgt tgtagaccca
     1501 acagatcctg aacggatctg gatcgcctgt tcaggttttg aggctgacca gaaagtgctt
     1561 tgctccaata gccgagggga tgtcgggtcg tgggtgaatt gggaccccaa tggaaccctg
     1621 cccaacctcc cggtgaacga tctgatctac cagaagggaa ctgatggggg actatatgtg
     1681 gccatggatg ttggggtcta ttaccggaac aactcgtcgg aagattggac cccctactac
     1741 gagacgcttc cgaatgtcca ggtcaatgat ctcgatatca actattgtgc aggcaagatc
     1801 cgcgcaggca cctacggtcg cggcctctgg gaaagcgacc tcgccgagcg gccaacggtg
     1861 gagaaggtcg tctcagcctc tgtcacttgg gacttccacc gcaacctcgc gcaagacttg
     1921 cacgtcacca acaacgccac cctcaccatc tccagcaccg tcaatttcgc gcccgacaca
     1981 cgcctcatta tcgaccccgg cgctcatgtg atcgtactgg acggtggtag actcacgaac
     2041 ctctgcggtg aagactggga ccgcgtggag gtgcgtggca acacttcggt tgaccaagtt
     2101 ccgctgaccg atcaaggata tctggaactg cgggcgggtg cagtggtgga acatgtccgc
     2161 aacggcgtat gccttttcgg aacggatgcc gagggcgatc ccgatctggc caccaccggc
     2221 ggcgtggtcc ggagcatcgg cacggccacc aacccggtga tcttccggga ttgcaaacgc
     2281 gggatctggg ccaaggacta caacttctac caacggtcca aggtcttgta cacgaacatg
     2341 gagtcctcct tggacggcac gatcgaccac atccgtcttg agggcttgcg gaacttcccg
     2401 atcaaccact cctcgttcac ggcggtaggg ttccacgata cccccgcgcc caccggcatc
     2461 cgttcattcg actctcgctt caccgtgcag gactgctcct tcgcgaactt gacaacgggc
     2521 gtccgcgcca gcgatgcgac gcttgtcaaa ccctgttacg tccgcaacaa ctccttcgac
     2581 cattgcgatc ggggcatcac cttgaacggg gtgtacaacg cggaggtcac tggcaacacc
     2641 ttcctcatcc cagccagcgg tggtagctgg atccccgacc ccagcatgtt ctactacccc
     2701 atcccgtacg gtgcctacct gtacgattgc gagggctacg aggtggaggg caacgatttc
     2761 atgggtacct cagcccatgg caacatcggc ctgctcatcc gaggcagcga ccaattgccg
     2821 aacgaattcg cgctgaacac cttcaagaag ctctatgtgg gcagccaggt gcagggctac
     2881 aaccgcagcc cggacgacgg cctgaagttc cgctgcaacc tctacggcaa cacggatggc
     2941 accgatggtt gtgtgtacga catggccctc accaccgatg atgcggcaat cgcgcctgtt
     3001 cagggcagcg tggatgaccc cgccgggaac cgcttctatc cgaattgcac caccagcggg
     3061 gaggagacgg acatctacgt ggatcaccag tcatccagtg ggttcctcta tttccatcat
     3121 gaagatgaat tcacagaccc cggctgcatt acatcggcgg tcatcccaca agcaacggac
     3181 tatggcttcg acgagagcgg accaggaact tcctgccctg atgatccaag ccatgttatc
     3241 agtatcagcg cccacaaatc ggccttccat gcggccacgg gtccttacgt ggcgctaaaa
     3301 gccgtgtacg acggtgaagt gaacggtggg aatacggagg atctggtcca catggtggcg
     3361 gatgagaaca tcccttcgtt cacgctccgc aacgccttgc tggccgcctc accaagattg
     3421 aatgattcag tggtgatccc agccatcctt cgggaccccg cgatggacga aggtcatctt
     3481 gtccaggtac tgctggccaa ctccccgctc tcagcggacg cgggccaggc agtaaagggg
     3541 agctatgtgg aagaactcta cacggaactg tgggaacagc agcagaatca tgaactccgt
     3601 tggactacgc tggtggaggc agagctttca ttgctgcgca ttggcgcaga gcgttcccgc
     3661 cacgactatt tccgcgggct attggagaat gacagcacgg gcatcgcgct gatgagcatg
     3721 gacgaggtgg tgaatgacac ggttgttccc gccacaccca tggagcagat cgcgctgctg
     3781 atagcccagg cagattggac tgaagctgac gatcggctcg ccgacccgaa cgcctacggc
     3841 ctacgcgaag accaggctgt ggtaatgact gttgtgcgac tgatcac
//
LOCUS       DAOQOC010000128         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10864_length_3880_cov_3.623529, whole genome shotgun sequence.
ACCESSION   DAOQOC010000128 DAOQOC010000000
VERSION     DAOQOC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10864_length_3880_cov_3.623529"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PL106_03075"
     CDS             complement(<1..227)
                     /locus_tag="PL106_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32079.1"
                     /translation="MRNTLLLLPILAANTLFAQRTFDVTIADRTCHMKRYGFVMYTRG
                     DGPAMDSLAAERLQQEHLDHQAEQTRCGLIMT"
     gene            complement(238..804)
                     /locus_tag="PL106_03080"
     CDS             complement(238..804)
                     /locus_tag="PL106_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNA32080.1"
                     /translation="MSGTANIPHERIQALTMEPIVLVPYDERWPILFSEEKARLERTL
                     PAGSFSRIEHIGSTAVHGLSAKPVIDIQVEVASLDRVRRELVPIMEDIAYEFIWRPTM
                     GESAPFYAWFIKRNPAGDRIFHVHMVEPDQASTDRILFRDHLRSHPADRAAYEALKHA
                     LAARHPDDRAAYTKNKTDHINRVLARAR"
     gene            complement(801..1625)
                     /gene="fdhD"
                     /locus_tag="PL106_03085"
     CDS             complement(801..1625)
                     /gene="fdhD"
                     /locus_tag="PL106_03085"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNA32081.1"
                     /translation="MTARIVPTNVQRFQRGSPVTTEDLLVAEEPLEIRLGHGPGHDRK
                     ETRLSVTMRTPGHDEELALGFLFTEGIITDPDQLVRVEHCTHVKDEERGNVVRAELRP
                     DVDVDPAAWQRNFYTTSSCGVCGKSSIEAVRVRCDKPLVPWGALEPAVITVLPARMRA
                     SQTVFKHTGAIHAAALFDRAGELLLLREDIGRHNAVDKVIGAALLQHRPMDASMLLVS
                     GRAGLELVQKCVVAGIPLMSAVGAPSSIAVRLARESGLTLLGFLHEERFNVYSGVT"
     gene            complement(1679..2251)
                     /locus_tag="PL106_03090"
     CDS             complement(1679..2251)
                     /locus_tag="PL106_03090"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNA32082.1"
                     /translation="MSKRPIIRCSWPGNDERMIQYHDTIWGTPEHDDRKLYAKLVLDG
                     AQAGLSWKTILHRSEGYRKAFDDWNVERIARYGPKDVARLLTDEGIIRNRAKVASAIN
                     NAQAWLRIMDRGHGSFDDFLWKHVDHKTLVNHWTNGKPVPASTPISDALSKDLKKAGF
                     SFVGSTIIYAFMQAIGMVDDHAEECFRKAR"
     gene            complement(2389..2868)
                     /locus_tag="PL106_03095"
     CDS             complement(2389..2868)
                     /locus_tag="PL106_03095"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNA32083.1"
                     /translation="MATSDEIFLREAISLARTGMERGDGGPFGCVIVKDGSVIGRGNN
                     RVTSTNDPTAHAEVVAIRAACAALRSFQLDGCVLYTSCEPCPMCLGAIYWARPDRIVF
                     AATRADAADAGFDDQLIYDELPLAPELRRISTTSSLLRNEACALFSTWKEKEDRVRY"
     gene            2951..>3880
                     /locus_tag="PL106_03100"
     CDS             2951..>3880
                     /locus_tag="PL106_03100"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNA32084.1"
                     /translation="MPIKSKLPHVGTTIFTVMSKLATECGAINLSQGFPDFPIDQTLS
                     DLVHAAMRAGYNQYAPMPGLPALREAITAKVQRMYGYGYDPDAEVTVTAGGTQAIFTA
                     IGATVHRGDEVIIVDPAYDCYAPTVELFGGSPVHVRLGADMSFDHDAVRAAITPRTRM
                     LMINTPHNPAGTVLSDADMHRLADLLRGTDILLLSDEVYEHLVYDGRKHATVINHPEL
                     RDRAFVIFSFGKVFHATGWKMGYALAPKDLMTEFRKVHQFNVFSVNTPAQHALASYLR
                     EPAHYEELPAFYQAKRDRFAAGMEGGRFKLLPCA"
BASE COUNT          694 a         1176 c         1261 g          749 t
ORIGIN      
        1 gtcatgatga ggccgcaccg ggtctgctcg gcctggtggt ccaggtgctc ctgttgcagc
       61 cgctcggccg cgaggctgtc catcgcaggg ccatcgccgc gcgtgtacat cacgaacccg
      121 tatcgcttca tgtggcaggt gcggtcggcg atggtcacat cgaaggtgcg ttgcgcgaag
      181 agtgtgttcg cggcgaggat gggcaggagc agcagggtgt tgcgcatgct gtccggttca
      241 gcgcgccctg gccagcacac ggttgatgtg gtccgtcttg ttcttcgtgt aggcggcgcg
      301 atcgtcggga tggcgtgcag cgagggcgtg cttgagcgcc tcgtaggccg cccggtccgc
      361 tggatgcgag cgcaggtggt cacggaagag gatcctgtcc gtactggcct gatcgggttc
      421 caccatgtgg acatggaaaa tgcgatcccc ggcagggttg cgtttgatga accaggcgta
      481 gaacggggcc gactcgccca tggtcggccg ccagatgaac tcgtaggcga tatcctccat
      541 gatcggcacc aattcgcggc gtacgcggtc caggcttgcc acctccacct ggatatcgat
      601 gacgggtttt gcgctgaggc cgtgcacggc agtgcttccg atgtgctcaa tgcggctgaa
      661 tgaaccagcg ggcagggtgc gctccagtcg tgctttctcc tcagaaaaga gtatcggcca
      721 acgttcgtca tacggcacaa ggacgatggg ctccatggtg agggcctgga tccgttcgtg
      781 cgggatgttc gcggtgccgc tcatgtgacg ccactgtaca cgttgaaccg ttcctcgtgg
      841 aggaacccga gtagcgtgag cccgctttcc cgggcgagcc ggaccgcgat ggaggaaggc
      901 gcgcccacgg cgctcatcaa cgggatcccg gccaccacgc acttctgcac cagctccagt
      961 ccggcgcggc cgctgaccag gagcatgctg gcgtccatgg gcctgtgttg gaggagggcc
     1021 gcgccgatga ccttgtccac ggcgttgtgc cggccgatat cctcgcgcag caggagcaat
     1081 tcacccgccc gatcgaacag cgcggcagca tggatggctc cggtgtgttt gaacacggtc
     1141 tgggacgccc gcatgcgggc gggaagaacg gtgatgacgg cgggctccag cgccccccac
     1201 ggtacgagcg gcttgtcgca tctcacgcgc accgcctcga tgctgctctt gccgcacaca
     1261 ccgcaactgc tggtggtgta gaaattccgt tgccaggccg cagggtccac gtcaacatcg
     1321 ggccggagct cggcacgcac cacattgccg cgttcctcgt ccttcacgtg ggtgcaatgt
     1381 tccacgcgta ccaattgatc gggatcggtg atgatgccct cggtgaacag gaaccccagc
     1441 gcgagctcct cgtcatggcc cggggtgcgc atggtcacgc tcaggcgggt ctccttgcgg
     1501 tcatgtccgg gaccatggcc gagccggatc tccaagggtt cctccgccac gagcagatcc
     1561 tcggtggtga ccggggatcc gcgttggaaa cgttggacat tcgtcgggac tatccgcgcg
     1621 gtcatggtcg aaaattacga aggcgcgcag gatgagccgg tccgttgtgg tccagcggtc
     1681 atcgggcctt ccggaagcat tcttccgcat gatcatccac catgccgatg gcctgcatga
     1741 aggcgtagat gatggtactg cccacaaagg agaaacccgc tttcttcagg tccttgctca
     1801 aggcatcgga gatcggtgta ctggccggaa cgggttttcc gttcgtccaa tgattcacga
     1861 gggtcttgtg gtccacatgc ttccagagga agtcatcgaa ggagccgtgc ccccggtcca
     1921 tgatgcgcag ccaggcctgt gcgttgttga tcgcgctggc cactttggca cggttgcgga
     1981 tgatcccctc atcggtcagt aaccgtgcga catccttcgg gccatagcgc gcgatcctct
     2041 ccacgttcca gtcgtcgaac gccttccgat agccttcact gcggtgcagg atcgtcttcc
     2101 aactgagccc ggcctgcgca ccatcgagca cgagcttcgc gtatagtttg cggtcgtcgt
     2161 gttcgggcgt gccccagatg gtgtcgtggt actggatcat gcgttcatcg ttcccgggcc
     2221 aggaacagcg gatgatcggt ctcttcgaca tggttagtgg tgcggcaagg atgagggctt
     2281 gaaggtaaga agctcaagct tttggacggt gtaccagggt gcgaacaccg gagttgggat
     2341 accgggccaa ctcggggatg cggatcacgc accggacaag cagttcgttc agtagcgtac
     2401 cctgtcctcc ttctctttcc aggtactgaa gagggcacag gcctcgttgc gtaataatga
     2461 cgaagtagta gaaatgcggc ggagttccgg ggccagcggc agttcgtcgt agatcaattg
     2521 gtcatcaaag ccggcatcag cggcatcggc ccgggtggcg gcgaagacga tgcggtcggg
     2581 ccgggcccag tagatggcgc ccaggcacat gggacaaggc tcgcaactgg tatagaggac
     2641 gcagccatcg agttggaacg accgcagtgc agcgcacgcg gcgcggatgg ccaccacttc
     2701 ggcatgcgcg gtggggtcgt tcgtgctggt cacccggttg ttgccccgcc cgatcaccga
     2761 gccgtccttc acgatcacgc agccgaaggg accaccatcg ccccgctcca tgcccgtgcg
     2821 tgccaagctg atcgcctcgc gcaggaagat ctcatcactg gttgccatgt cgcgaagatc
     2881 cggacttcgg cgcgtaatac ggatatggtc ccggtgacac ttcgggaggg ccctgtactt
     2941 tcgggccccg atgccgatca agagcaagct gccccatgtg ggcaccacga tcttcacggt
     3001 gatgagcaag ctggcgaccg aatgcggtgc catcaacctc agccagggct ttccggactt
     3061 cccgatcgac caaacgttga gcgacctggt acatgccgca atgagagcgg gctacaacca
     3121 gtatgcgccc atgcccggcc ttcccgccct gcgcgaggcc atcaccgcca aggtgcaacg
     3181 gatgtacggg tacggatatg atcccgatgc ggaggtgacg gtgacggcag gggggacaca
     3241 ggccatcttc accgccatcg gtgccacggt gcaccggggg gatgaggtca tcatcgtcga
     3301 tcccgcgtat gactgttacg cgcccacggt ggaactgttc ggggggagcc cggttcatgt
     3361 caggttgggc gccgacatga gcttcgacca cgatgcggtg cgggcggcca tcaccccgcg
     3421 cacgcgcatg ctcatgatca acacgccgca caacccggcg ggcaccgtcc tgagcgatgc
     3481 ggacatgcat cgtctggccg acctgttgag aggcaccgat attctcctgc tgagcgatga
     3541 ggtctatgag catctggtat acgatggcag gaaacacgcc acggtgatca accacccgga
     3601 gttgcgcgac cgcgccttcg tcatcttcag cttcggcaag gtctttcacg ccacgggctg
     3661 gaagatgggc tatgcgctgg caccgaagga cctgatgacg gagttccgca aggtgcacca
     3721 gttcaatgtc ttcagcgtga acacaccggc acagcacgcc ttggcctcgt atctccgtga
     3781 gcccgcgcat tacgaggaac ttccggcctt ctaccaggcc aagcgcgatc gtttcgcggc
     3841 gggcatggag ggcgggcgct tcaagttgct tccctgtgcc
//
LOCUS       DAOQOC010000129         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_10878_length_3878_cov_3.381114, whole genome shotgun sequence.
ACCESSION   DAOQOC010000129 DAOQOC010000000
VERSION     DAOQOC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10878_length_3878_cov_3.381114"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="nadC"
                     /locus_tag="PL106_03105"
     CDS             complement(<1..683)
                     /gene="nadC"
                     /locus_tag="PL106_03105"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNA32085.1"
                     /translation="MLPPGTDDLVRRTLLEDIGEGDHTTLSTIPADVRGAAHLLVKQE
                     GVLAGVELAEAICLHFDPALRLRTLIQDGAAVHAGDVAFTLTGPARSILIVERTVLNF
                     MQRMSGIATLTRRFVEAVNGTGCKVLDTRKTTPTLRAIEKWAVRIGGGHNHRNGLYDM
                     VMIKDNHADMAGGIPQAIQAAQRYLKEHDMRLPIEVETRNLSEVEQVLSIPGVQRIML
                     DNFGLDDLRT"
     gene            complement(689..1081)
                     /locus_tag="PL106_03110"
     CDS             complement(689..1081)
                     /locus_tag="PL106_03110"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNA32086.1"
                     /translation="MKRILLSLLSAMVAHLLIAQSDVKDQVTAALGSGDVNTLSEKLV
                     ANVDLTVLSTSDYYSKAQATGILRKFYEGHEPKGLRIEHEGTSKMGDRYCIGQLNTAN
                     GVFRVTFFLKKTGEVTQVKQLRIEPSNK"
     gene            1168..1629
                     /locus_tag="PL106_03115"
     CDS             1168..1629
                     /locus_tag="PL106_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNA32087.1"
                     /translation="MKTRLLMVGRTERGFVAEGLDHYLGRIRHWSPVDEVVVAKSDRT
                     TPELQRAEEEKNLLRAIPAGVRLVVLDETGRSLDSRAFARKLGDWRDQGAREVCFVVG
                     GAYGLTDAVRNKADLLLSLSPMTFPHQLVRVLLAEQLYRAWAILKGTGYHH"
     gene            complement(1641..2354)
                     /locus_tag="PL106_03120"
     CDS             complement(1641..2354)
                     /locus_tag="PL106_03120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA32088.1"
                     /translation="MKLRFERRKPRHTLLSICYRLAKLPFASARGKMRFYLDLNWIGW
                     RLAIEQAERMYGFGESSMRRKNLEFLLARMQSGARVLDLGCKYGEISAIVAEKAERVI
                     AVDHDPQALEKARAAHPATNLEFIHADAFDVLEKETAPVDLVILSHILEHLEKPEAML
                     ARLKAHATHCYIELPDLDASYLNHYRQLAGARLQYTDNDHIWEFDRDSLREVIESAGW
                     KILDSEYRYGVQKHWCGRA"
     gene            complement(2351..>3878)
                     /locus_tag="PL106_03125"
     CDS             complement(2351..>3878)
                     /locus_tag="PL106_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA32089.1"
                     /translation="EPSAPFRDRAISKIGVPSDRLRLGSVEDADYPAESFDFINLCVV
                     LEHIGDPGAALARALTWLKPGGVIHVEVPSSDWLIVRMTNLYYKLRRSECVGNLSPMH
                     APYHLHEFTVGSFRAHGRTNGYIVERYAHYVCPTYLPKVLDIIARPLMAWTRTGMEVA
                     VWLRKEGTPPDDLRAPDREPRSATPVDASGSTKTGSGPIKVPGTPRPCPQCGTSERTP
                     IGAMAPHHLARCNGCGLVYTALEPSTGDLLAFYSRYLTVEDISPVTILRYEEVLDSFE
                     PFRKNGRILETGCGSGIFMQRAAMRGWEVHGTEIGAHAIAVAGGRGVRMIDGPLDPKN
                     YPSGHFDIVCSIEVIEHLRDPRTELRNVMEVLRPGGLFYVTTPNFNCLARRLSPGDWN
                     VASYPEHLTYFTPRTLDRMLTSAGLRKRRLTTTGFSIYRWKVRHDRSAQNKNAAQKKE
                     ESIRMALEQKAHLRLAKRFTNAVLDLLRLGDSMKAGYVKPETDPGRIEPGPSNRMPER
                     "
BASE COUNT          674 a         1189 c         1214 g          801 t
ORIGIN      
        1 gtacgaagat cgtccaatcc gaagttgtcc aacatgatcc gttgaacacc cggtatggaa
       61 agcacctgct ccacttcgct gaggttgcgc gtctccacct cgatcgggag ccgcatgtca
      121 tgctccttca ggtagcgctg tgccgcttgg atggcctgcg gtatgccacc ggccatgtcc
      181 gcgtggttgt ccttgatcat caccatgtcg tacaagccgt tccggtggtt gtgccctccg
      241 ccgatgcgca cggcccattt ctcgatcgcg cgcagggtgg gcgtggtctt gcgcgtatcc
      301 agtaccttgc atccggtacc gttgaccgcc tccacgaaac gccgggtgag cgtggcgatg
      361 ccactcatgc gctgcatgaa gttgagcacg gtgcgctcca cgatgaggat gctgcgcgcc
      421 ggaccggtga gggtgaatgc cacgtcgccc gcgtggacgg ccgcgccatc ctggatgagc
      481 gtgcgcagcc gcaatgccgg atcgaagtgc aggcagatcg cctcggccag ttccaccccg
      541 gccagcacgc cctcctgctt caccaacagg tgcgccgcac cgcgcacatc ggccggtatg
      601 gtgctcagcg tggtgtgatc gccctcgccg atgtcctcca gcagcgtgcg acgaaccagg
      661 tcgtccgttc cgggtggcag caccgacctt atttgttgct gggctcgatg cggagctgtt
      721 tcacctgcgt gacctctccc gtcttcttca ggaaaaaggt gacacggaat acgccattgg
      781 ccgtgttcaa ttggccgatg cagtaccggt cgcccatctt gctcgtgcct tcgtgctcga
      841 tccgtaggcc cttgggctcg tgtccctcat aaaatttgcg caggatcccc gtggcctggg
      901 ccttgctgta gtagtcgctg gtgctcaaaa ccgtgaggtc aacgttggct accagcttct
      961 cgctcaacgt gttcacgtcg cccgagccga gggcggcggt cacctggtcc ttcacgtccg
     1021 actgcgcgat gagcaggtgt gcgaccatgg cggagaggag ggaaaggagg atgcgtttca
     1081 ttggtgttgc tgaagcgtga gcgaattttg caaacatcag gccacaggag ggcggtaccc
     1141 cgggcttact tgtgaacaac cccgtccatg aagacacgat tgctgatggt gggccgtacg
     1201 gaacggggtt tcgtggccga aggcctggat cattacctgg gccgtatccg gcattggagc
     1261 cctgtggatg aggtggttgt ggcgaaaagt gaccgcacca cgcctgagtt gcagcgcgcg
     1321 gaggaagaga agaacctgtt gagagccata cctgctggtg tacgactggt ggtgcttgac
     1381 gaaacgggcc gctccttgga cagtcgtgcc ttcgcccgga aattgggcga ttggcgcgat
     1441 cagggtgccc gggaagtgtg ttttgtcgtc ggaggcgcgt acggccttac cgatgcggtg
     1501 cgcaacaagg ccgacctctt gctgtccctt tcgcccatga cgttcccgca ccagctggtg
     1561 cgcgtgctgt tggccgaaca gttgtaccgc gcctgggcga tcctgaaggg tacgggttat
     1621 caccactgag tccgacggca ctatgcccgc ccgcaccagt gcttctgcac gccgtaccga
     1681 tattcactat ccaggatctt ccagcccgca ctctcgatca cctcacgcaa cgagtcgcgg
     1741 tcgaattccc agatgtggtc gttgtccgtg tactgcaacc gggccccggc cagctgccgg
     1801 tagtggttca aataggaagc gtccagatcg ggcagctcga tgtaacagtg ggtggcgtgg
     1861 gccttcagcc gggccagcat cgcttcgggt ttctccagat gttccaggat gtgggacagg
     1921 atcaccaggt ccaccggcgc cgtttctttt tccagaacgt cgaaggcatc ggcgtggatg
     1981 aactccaggt tcgttgccgg gtgcgctgcg cgtgcttttt ccaatgcctg cggatcatgg
     2041 tccacggcga tcacacgttc ggctttctcc gccacgatcg cgctgatctc cccgtatttg
     2101 cagcccagat cgagcacccg tgccccggac tgcatccggg cgaggaggaa ctccaggttc
     2161 ttgcggcgca tggagctctc gccgaagccg tacatgcgtt cggcctgctc aatggccaga
     2221 cgccagccga tccaattcag gtcgaggtag aaacgcattt ttccccgggc gctcgcgaag
     2281 ggcagtttgg ccaggcgata acagatggaa agcagcgtat gccgcggctt ccgacgttcg
     2341 aaccggagtt tcatcgctcg ggcatcctgt tgctcggccc gggctcgatc cttccagggt
     2401 ccgtttcggg tttgacataa ccagctttca tgctgtcccc gagccgaagc aggtccagca
     2461 cggcattggt gaaccgcttg gccaggcgca ggtgggcctt ttgctccagc gccatacgta
     2521 tggattcctc tttcttctgc gcagcgttct tgttctgcgc actacggtcg tggcgcactt
     2581 tccatcggta tatgctgaat cccgtggtgg tcaaccgtcg cttgcgcagg cctgctgagg
     2641 tgagcatccg gtccagtgtg cgcggcgtga aataagtcag gtgttccgga tagctcgcca
     2701 cgttccaatc accgggtgaa aggcggcggg caaggcagtt gaagttcggt gtggtcacgt
     2761 agaacagacc tcccggtcga agcacctcca tcacgttccg gagttcggtc cgagggtcgc
     2821 gcaagtgctc gatcacttcg atggagcaca cgatgtcgaa atgtccggat gggtagtttt
     2881 tcggatcgag cggaccatcg atcatccgca caccgcgtcc accggccacc gcaatggcgt
     2941 gcgcgccgat ctccgtgccg tgcacctccc aaccccgcat ggcggcccgt tgcatgaaga
     3001 tgcccgagcc gcaacccgtc tccaggatac ggccgttctt gcggaatggc tcgaaactgt
     3061 ccagcacttc ctcgtagcgc aggatcgtta ccggggaaat atcctcgacg gtgaggtagc
     3121 gcgagtagaa tgcgagcaga tcccccgtcg agggctcgag tgccgtgtac acgagcccgc
     3181 acccattgca gcgggcaagg tgatgcgggg ccatggcacc gatgggcgtc cgctccgagg
     3241 tgccgcattg ggggcacggc cggggtgttc ccgggacttt gatcgggcca cttccggtct
     3301 ttgtactccc gctcgcatcc accggggtcg cggaacgtgg ttcgcggtcc ggagcgcgca
     3361 ggtcgtccgg tggtgtaccc tctttgcgca accagaccgc cacctccatc ccggtgcggg
     3421 tccaggccat caaggggcgt gcgatgatat ccaggacctt ggggaggtag gtcggacaca
     3481 cataatgggc gtagcgttcc acgatgtagc cgttcgtacg gccatgggct cgaaaggatc
     3541 ccaccgtgaa ttcatgcagg tggtaggggg catgcatcgg gctcaggttc cccacgcatt
     3601 ccgaacggcg cagcttgtaa tacaggttcg tcatccggac gatgagccag tcggacgagg
     3661 gtacctccac atggatgacg ccgcccggtt tcagccatgt gagcgcccgg gccagcgccg
     3721 ctccgggatc cccgatgtgc tccagcacca cgcacaggtt gatgaagtcg aatgactccg
     3781 ctggataatc cgcatcctcc accgacccca aacgaagacg gtccgaaggc acgccgatct
     3841 tgctgatggc ccggtccctg aacggggccg agggttcc
//
LOCUS       DAOQOC010000130         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11120_length_3833_cov_4.172843, whole genome shotgun sequence.
ACCESSION   DAOQOC010000130 DAOQOC010000000
VERSION     DAOQOC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11120_length_3833_cov_4.172843"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PL106_03130"
     CDS             <1..759
                     /locus_tag="PL106_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNA32090.1"
                     /translation="KLVPMPKETRLMLVPFADAEKACEAVSAVFRAGITPSALEFMER
                     DAIAWTMRFTDGLPAQLSTGPGAYLLIEVDGDHGDVLMRDCETILGVMEEHGGGETLF
                     GETTAEKEALWRLRRSVPVSVKAHSVYKEEDTVVPRYALSRLIAGVKAIGARYGFSSV
                     CYGHAGDGNLHVNIIKGDLSDAFWNEELPKAIREIFELTVSLGGTLSGEHGIGLVQRP
                     YMDIAFDPVQLGLMRAIKGVFDPQGIMNPGKVLP"
     gene            complement(1019..1612)
                     /locus_tag="PL106_03135"
     CDS             complement(1019..1612)
                     /locus_tag="PL106_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32091.1"
                     /translation="MRVGLFALLLIGACARPVEDVVIPDRDGKPSVRLRRAGEAKDGP
                     ATLYWPTGEVRLKGLYANDRRVGWWGGYLRDGHWHSWTHYRNGLKDGLRVYWDSLGRV
                     VRSERFAEGKHNGLFVRRSADGRIAQCSTYADDLLEGPHIQWYDDRGGSVVKGQYHKD
                     RPDGLWTEYDTTGRMIWQGVFKAGVVERMLYGKRRRH"
     gene            1651..2340
                     /gene="mnmD"
                     /locus_tag="PL106_03140"
     CDS             1651..2340
                     /gene="mnmD"
                     /locus_tag="PL106_03140"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNA32092.1"
                     /translation="MGSLYVPPADLVPYRTADGSLTLRSVILGEQYHSKFGAVQESTH
                     VFIKAGLEHAAREVVDVLEVGLGTGLNLLLTWIRCLEGKNRVRYTALEPFPLRVDQLE
                     ALGHCADLAWPGLHEPFIARMTDGHGEWHDAVGGLAFRLLPMHVAELDDENAYDIVYF
                     DAFAPDVQMEMWTEDVFQRMYRALRPDGVLVTYCAKGMVRRVMTSAGFIVERLPGPPG
                     KREMLRAMKLV"
     gene            2412..2891
                     /locus_tag="PL106_03145"
     CDS             2412..2891
                     /locus_tag="PL106_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA32093.1"
                     /translation="MDIHRFNIRVYGLLLHEGRVLLADERIQGRHITKFPGGGMEFGE
                     GPKECLIREIREEIGVEAFGLEHFYTTDFFQESAFRPGDQIISIYFTMRVMDPGSIPV
                     NEDPFAFGRAEGDEEVFRWLALARAVPEDASLPIDRVVLGLLKERFNQHGGAAPSNS"
     gene            complement(2932..3507)
                     /locus_tag="PL106_03150"
     CDS             complement(2932..3507)
                     /locus_tag="PL106_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32094.1"
                     /translation="MHTLEPFYNWQHRYNAEEDDRSPFFGTEHSEFEFINSVYDHVIH
                     PQWDEFGSRTLYLKTLFVDYEEGYAIIEMIGEWNDLLYNDVMLLKRDVIEPMMSHGIV
                     RFILIGENVLNHHPSDEEYYSEWFEEVSDADGWIALLNFREHVRDDLKAANIDRYFLL
                     GGQLDMMDWRTFEPEDLYERVSAQVMKRLGA"
     gene            complement(3590..>3833)
                     /locus_tag="PL106_03155"
     CDS             complement(3590..>3833)
                     /locus_tag="PL106_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNA32095.1"
                     /translation="RMKSGTAQKLILNMISTSVMIKLGRVQGNRMVDMRMSNNKLIDR
                     GTRMVMDALSIPYEQANELLRQHGSVRQAMEAGNRP"
BASE COUNT          751 a         1120 c         1184 g          778 t
ORIGIN      
        1 aagctggtgc ccatgccgaa ggagacacgt ctgatgctgg tccctttcgc cgatgccgag
       61 aaagcctgcg aagccgttag cgccgtgttc cgggcgggca tcaccccgag tgcgttggag
      121 ttcatggagc gcgatgcgat cgcgtggacg atgcgcttta ccgatggcct gcccgcccaa
      181 ctgtccaccg gtccgggcgc ctacctgctc atcgaagtgg atggcgatca tggcgacgtg
      241 ctcatgcgtg attgtgaaac gatactcggt gtcatggagg agcacggtgg gggcgaaacg
      301 cttttcgggg agacaacggc ggagaaggag gcactctggc ggttgcggcg cagcgtcccc
      361 gtgagcgtga aggctcacag tgtgtacaag gaagaggaca cggtagtgcc gcgctacgcg
      421 ctatcgcggt taatagccgg agtgaaggcc atcggtgcgc gatacgggtt cagcagtgtg
      481 tgctacgggc acgccggcga cggcaacctg cacgtgaaca tcatcaaggg cgacctgagc
      541 gatgcgttct ggaatgaaga gctcccgaag gcgatccggg agatattcga actcacggta
      601 tcactgggtg gaacgttgag cggcgagcac ggtatcgggc tggtgcagcg gccctacatg
      661 gacatcgcct tcgatcccgt tcaattggga ttgatgcggg ctatcaaagg ggtgttcgac
      721 ccacagggga tcatgaaccc gggcaaggtt ctgccgtgag gtgggatggg tgaggacaaa
      781 gagggaatga cggccgaatc ccatccttca tttgcgtggc tggccaagca agcatgcgtg
      841 aacgactgat acacagttgg attgacggat atcgttcatg atcgggctcc gttaatgctg
      901 cttggaagcc tcttaatagg ctgatttccc tcaaatactt gcttggccag ccacgcaaat
      961 gagggcattc ccagagaaca cagggccaac tccatctttc acacacggcc ggatcgcgct
     1021 aatgccttct tctcttcccg tacagcatcc gttccaccac acccgccttg aaaaccccct
     1081 gccagatcat cctgccggtg gtatcatatt cagtccaaag accatccggg cggtccttat
     1141 ggtactgacc cttcaccact gatccgcccc gatcatcgta ccattggatg tgcggccctt
     1201 cgagcaggtc atccgcgtag gtgctgcatt gggcgatcct gccatcggcg gaacgccgga
     1261 caaagaggcc gttgtgcttg ccttcagcga accgctcgga gcgcaccaca cgccccaggc
     1321 tgtcccagta cacccgaagg ccatctttca aaccgtttcg ataatgcgtc catgagtgcc
     1381 agtgaccatc acgcagataa ccgccccacc agcccaccct tcgatcattc gcgtacagtc
     1441 ctttcaagcg cacttcgccg gttggccaat acaaggtggc cgggccatcc ttcgcctctc
     1501 ctgcacggcg caatcggacc gatggcttgc catcgcgatc gggaatgacg acgtcttcaa
     1561 caggccgcgc acaggcacct atcaacagga gggcgaaaag gccgacgcgc atgagtgcac
     1621 gaaggtatcc cgcaccgaac ttcgcggcca gtgggatccc tgtacgtgcc accggccgac
     1681 cttgtcccgt accggactgc ggacgggtcc ctcacgctca ggagcgtgat acttggagag
     1741 cagtaccata gtaagttcgg tgcggtccag gagagcaccc acgtgttcat caaggcggga
     1801 cttgagcatg cggccaggga ggtggtcgat gtactggagg tggggcttgg aacggggctg
     1861 aacctgctgc tcacctggat ccgatgtctt gagggcaaga accgggtgcg ttatacggcc
     1921 ctcgagccgt tccccttgcg agtcgaccag ctcgaagcgt tgggccattg cgccgacctg
     1981 gcatggccgg gtctccacga accttttatc gcgcggatga cggatggcca cggcgaatgg
     2041 catgacgctg tcggcgggct ggcattccgg ttactgccca tgcacgtggc cgaactggat
     2101 gacgagaacg cttatgacat cgtctacttc gacgcgttcg cgcccgatgt acagatggag
     2161 atgtggacgg aggatgtctt ccaacgcatg tatcgtgcac tgcgtccgga cggggtcctg
     2221 gtgacgtact gtgccaaggg catggtccgt cgggtgatga cctccgcagg cttcatcgtg
     2281 gaacgactgc ccgggcctcc tggcaagcgg gaaatgctgc gggcgatgaa actggtgtga
     2341 gcctcgtgcc actcttgatc catgaggtca accgacaggg atcgaacggg gcgtcgcacc
     2401 atcttcgccc catggatatc catcgcttca acatccgtgt gtacgggctt ctccttcacg
     2461 agggaagggt gctcctggcc gacgagcgga tccaaggccg tcacatcacc aagttccccg
     2521 ggggcggcat ggagttcggt gaggggccga aggaatgtct gatacgcgaa ataagggaag
     2581 aaattggagt ggaggcgttc gggttggaac atttctatac cacggacttc ttccaggaga
     2641 gtgcattccg cccgggggat cagatcatca gcatctactt caccatgcgg gtcatggatc
     2701 cgggatcgat ccctgtgaac gaagaccctt tcgcgttcgg ccgtgcggag ggcgacgagg
     2761 aggtcttccg gtggctggcc ctcgcaagag cggtgccgga agatgcctcc ctgccgatcg
     2821 accgggtggt gctgggcctg ttgaaggagc gcttcaacca gcacggagga gcagcccctt
     2881 ccaatagctg acagtgcggt gcatgccgcg aacgggctgg cacggcacgg ttcaagctcc
     2941 caggcgcttc atcacctggg cactcacccg ctcgtacagg tcctcgggtt cgaacgtgcg
     3001 ccagtccatc atgtccaatt ggcccccgag caggaaatag cgatcgatat tggcggcctt
     3061 caggtcgtcg cgcacatgct cacggaagtt gagcagggcg atccatccgt ccgcatccga
     3121 cacctcttcg aaccattcgc tgtagtactc ctcatcgctg gggtggtggt tgagcacgtt
     3181 ctcgccgatc aggatgaagc gcacgatgcc gtgtgacatc atgggttcga tcacatcacg
     3241 cttgagcagc atcacgtcgt tgtacaggag gtcgttccac tccccgatca tctcgatgat
     3301 cgcgtatcct tcctcatagt cgacgaagag cgtcttgagg tacagcgtac ggctcccgaa
     3361 ttcatcccat tgcggatgga tcacgtggtc gtacacggag ttgatgaact cgaactcgct
     3421 gtgctccgta ccgaagaatg gggaacgatc gtcctcttcc gcgttgtagc ggtgctgcca
     3481 gttgtagaac ggttcaaggg tgtgcatcgg aagggatggg cagcgtgcgg gggaggctcc
     3541 ctgcggcgaa gatcgttgat ggggccccat ccggcgcagg tcccgacgcc tacggccggt
     3601 tgcccgcttc catcgcctgg cggacgcttc cgtgttgtcg caacaactcg ttggcctgct
     3661 cgtacgggat gctcaaggcg tccatgacca tccgcgtacc gcgatcgatg agcttgttgt
     3721 tgctcatccg catgtccacc atccggttgc cctgcacgcg gcccaacttg atcatcaccg
     3781 aggtggagat catgttcagg atcagtttct gcgctgttcc gctcttcatg cgg
//
LOCUS       DAOQOC010000131         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11122_length_3833_cov_3.259132, whole genome shotgun sequence.
ACCESSION   DAOQOC010000131 DAOQOC010000000
VERSION     DAOQOC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11122_length_3833_cov_3.259132"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PL106_03160"
     CDS             complement(<1..1001)
                     /locus_tag="PL106_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA32096.1"
                     /translation="MKLVAAEVVDRIGRVSFHFFLLVTVFTTAAQDPPHKMYTVADGL
                     SSNTVYCAVQDNEGYMWFGTDAGACRFDGLRFERFTASQGLADNEVIGMHVDQKGRIW
                     FLGLNGRVSVFSDGAFVNERLGTDRIQGRHGLVSAADDGQGGVWVGGFDSELFHWSNG
                     RVTRLDLKVPGTTGTHGMVKLLRLSNGTLYAMRNAALFLIESDTARFLRRWDQGGAEY
                     PMAAVRNDRVLAFGRLGLLEVGLAKDRLITPLDRTMPPSGARLPFVDRDGSIWSGRED
                     GGVVRVMEHAGGAPTIQRFFIRSHINTIQQDQEGNIWWCTDGNGVFISRYAQLGIQQ"
     gene            complement(1253..1996)
                     /locus_tag="PL106_03165"
     CDS             complement(1253..1996)
                     /locus_tag="PL106_03165"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA32097.1"
                     /translation="MPIPNTDEHGRRRLVTALIPPVLVVSVLWIVFAFDRVYNLDLYR
                     FGVYPREAKGLLGIVVSPFVHAGLEHLVNNSAPILVLGWLLVYFYPKVAGPVTLACWG
                     FSGLWVWLTARENYHIGASGVIYGLAAFLFLSGVLRKLVPLMAVSLLIVFLYGSMVWG
                     VLPLEPHISWESHLSGAIGGLLMAWIYRKVPPAHVPPPIVLEDDPEEPELQVVTNNDP
                     GDEEPTTAAPVEPRPSADPLRFLDGGPTI"
     gene            2110..2571
                     /locus_tag="PL106_03170"
     CDS             2110..2571
                     /locus_tag="PL106_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32098.1"
                     /translation="MRTCAILSSVLACVALFVACGGGSDPEPEPAEQNSRSIADLPEH
                     KTSPLAQLMREMTAFGDTTRARLLRGEDLPPYPVHFKDMLTLESTPGMVDHTTYDPFA
                     IAYLYQLDSLYKVPVAERQQGFDELIQYCAACHGEVCPGPLVRINKLRMPG"
     gene            2809..>3833
                     /locus_tag="PL106_03175"
     CDS             2809..>3833
                     /locus_tag="PL106_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32099.1"
                     /translation="MVRTFIVLVATCTLLHASAQQGRGPRFPFTANKGQWPEQVLYRT
                     LTGQGALFVERDALTYVVRSGGHAHGHRPDHVNNERHDHAYRVHFVGGRATSHQGSDQ
                     QAWYENWFVGPESSWAGRVPVHAGARLTNLYPGIDLVLHGDDRLEYDLHVAPGADVDA
                     VALRYEGHDSIELRNGRLVITTSAGQVTEEAPVAWQDIDGKRRDVPCHFILTDGTVRF
                     ELPQGYDRRHPLTIDPVLTFSSFSGSTADNFGFTATYDNAGHLYGGGIVFGVGYPTTL
                     GVQQPNFGGNTIDMGITKFSPDGTQLIWSTYLGGLLGNESPHSLVVNSADELYVMGIT
                     GSADFPVT"
BASE COUNT          788 a         1236 c         1084 g          725 t
ORIGIN      
        1 aattgctgta ttccaagttg agcgtatcga ctgataaaga ccccatttcc gtcggtacac
       61 caccagatgt tgccctcttg gtcctgttgg atcgtgttga tgtggctccg tatgaagaac
      121 cgttggatcg ttggcgctcc gcctgcatgc tccatcacac ggaccacacc gccatcctcc
      181 cggccggacc agatgctccc atcgcgatca acgaacggca gccgcgctcc ggaaggtggc
      241 atcgtacgat ccaagggtgt gatgagccgg tctttcgcaa gccccacttc caacagtcct
      301 aagcgtccaa aggccagcac ccgatcgttc ctgaccgcag ccatgggata ttcggcgcct
      361 ccttgatccc acctgcggag gaaacgcgcc gtatcgctct caatgaggaa gagcgccgcg
      421 ttccgcatgg catacagcgt gccgtttgag agtcgcagga gcttcaccat cccatgcgtt
      481 cctgtggttc cgggcacctt gaggtccaag cgcgtcaccc tgccgttgga ccagtggaag
      541 agttccgagt cgaacccgcc tacccatacg ccaccctgac cgtcatcggc cgcggagacc
      601 aggccgtgac ggccctgaat gcggtccgtt cccaaccgtt cgttcacgaa ggctccatcg
      661 ctgaatacac tgacacgacc attcaggcca aggaaccaga tacggccctt ctggtccaca
      721 tgcataccga tcacctcatt gtccgccaga ccctggctgg ccgtgaaccg ctcgaaccgc
      781 aggccatcaa agcggcaggc gccagcatcc gtcccgaacc acatgtaccc ttcgttgtcc
      841 tgaacggcgc aatacaccgt gttgctgctc agtccatctg ccaccgtgta catcttgtgc
      901 ggcggatcct gggccgcagt agtgaacaca gtgaccagca ggaaaaaatg gaacgacaca
      961 cgcccaatac ggtcaactac ttcagccgcc acaagcttca ttgtgcgcga agttccaacg
     1021 gaacatcatt gcccggatcc atcaattgtg atgggcaggt caactgcgat gttccacatc
     1081 cggatcggtg cggcatgttc gaccacatct tccgatgaaa cctgacaact tgtgccgtgg
     1141 tgccaatagc ttgctatcgg acatgatgga cccggtcttc gatcctgcta aatcgaacac
     1201 caaaataggg atcgccactt ccccgccttg aacggccacc cccgaagcgc cttcaaatgg
     1261 tcggtccgcc gtccaagaac cgcaacggat ccgcactggg gcgcggttcc acgggtgccg
     1321 cagttgtcgg ctcctcatcc cctggatcgt tgttcgtgac cacctgcagt tcgggttcct
     1381 ctgggtcgtc ctccagcacg atgggcggtg gcacatgcgc cggtggcacc ttgcgataga
     1441 tccatgccat cagcaagcca ccgatggcac cggaaagatg actttcccag ctgatatgtg
     1501 gctccagagg caacacgccc cacaccatgc tgccgtacag gaagacgatc agcaacgata
     1561 cggccatcaa tggtaccagc ttccggagca caccactgag aaagaggaag gccgccaagc
     1621 catagatgac cccgctggcc ccgatgtggt agttttcgcg tgcggtgagc cagacccaga
     1681 gcccgctgaa accccagcat gccagggtca ccggacccgc caccttcgga tagaaataga
     1741 ccagcagcca gcccagcacc aggatgggag cactattgtt caccaaatgt tcaaggccag
     1801 catgtacgaa cggcgagacg acgatgccga gcagcccctt cgcctcgcgc gggtatacgc
     1861 cgaagcggta gaggtcgagg ttgtacaccc gatcgaacgc gaagacgatc cagagcacgg
     1921 acaccaccag caccggagga atgagcgcgg tgaccaagcg gcgacggccg tgttcgtccg
     1981 tgttgggaat gggcatgtgc atttgcgcgg gcgatcttcg ggccggaacg tacgggcgga
     2041 caacttggca gatcgcccgg tgaaggtcaa ctaagtttgc gacggtcacc acgaatcccg
     2101 cttattccca tgagaacctg cgcgatcctt tcttccgtcc tcgcgtgcgt agcgctgttc
     2161 gtagcctgtg gcggcggttc cgatcccgaa cccgaacccg cagaacagaa ttcgcggtcg
     2221 atcgccgacc tgcccgaaca caagacgagc ccattggccc agttgatgcg cgagatgacg
     2281 gctttcggtg acactacccg cgcgcgactc ctgcgcggcg aggacctgcc cccctacccg
     2341 gtccacttca aggacatgct cactttggag agcacacccg gcatggtgga tcataccacc
     2401 tacgatccct tcgccatcgc ctacctctat caactcgatt ccctgtacaa ggtgcccgtt
     2461 gccgagcgcc agcaaggctt tgatgagctg atccagtact gtgcggcctg ccacggtgag
     2521 gtgtgcccag gacctttggt gcgcatcaac aagctgcgca tgcccggatg atggcgtaga
     2581 tcattttccg ccatcccatg gaatgcaccc gcccaagtgg tgacgctact atgtgggcgt
     2641 actgaccgaa aggctttgct ggatcgggtc ctggcctgaa ttgtccgatc actacaatgg
     2701 ccccttggct ggatgttgga agagccaccc cggatgagtt gctcatcggc tctggcatcg
     2761 aagatgcgcc ttcccgcgtc ataaaacctg aaaccctaca ttgccagcat ggtgcgaacg
     2821 ttcatagtcc tggtggccac ctgcacattg cttcatgctt cagcccaaca aggccgtggt
     2881 ccgcgcttcc ccttcaccgc gaacaagggc caatggccag agcaggtgct ctaccggacg
     2941 ctgaccgggc agggggcgct gttcgtggag cgtgatgcgc tgacctacgt ggtgcgcagt
     3001 ggcggacatg cgcatggcca ccgaccggac catgtgaaca acgaacggca cgatcacgca
     3061 taccgcgtac atttcgtggg cggtcgcgcc acctcgcacc agggatcgga ccagcaggcc
     3121 tggtacgaga actggttcgt gggccccgaa agcagctggg ccggacgtgt gcctgtgcat
     3181 gccggagccc gactcaccaa cttgtatccg ggtatcgatc tggtccttca tggggacgat
     3241 cgtttggagt acgacctcca cgtggcgccc ggtgccgatg tggatgccgt ggccctgcgc
     3301 tacgaaggac atgacagcat tgaattgcgc aacggtcggc tcgtgatcac cacctcggcc
     3361 ggccaggtga ccgaggaagc gcctgtggcc tggcaggaca ttgatggcaa gcgacgtgac
     3421 gtgccgtgcc acttcatcct gacggacggc accgtgcgct tcgagttgcc ccaggggtac
     3481 gaccgccgcc accccttgac gatcgatccg gtcctcacgt tctccagctt ctcgggcagc
     3541 acggcggaca acttcggctt cacggccact tacgacaatg ccggccacct gtacggagga
     3601 ggcatcgtgt tcggtgtggg ctatcccacc accttgggcg tgcagcagcc gaacttcggt
     3661 gggaacacca tcgacatggg gatcaccaaa ttcagccctg acggtaccca gctgatctgg
     3721 agcacttacc tcggcggcct cttgggcaac gagagcccac acagcctggt ggtgaacagt
     3781 gcggacgagt tgtacgtgat gggcatcacc ggttccgccg acttccccgt tac
//
LOCUS       DAOQOC010000132         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11127_length_3832_cov_3.422822, whole genome shotgun sequence.
ACCESSION   DAOQOC010000132 DAOQOC010000000
VERSION     DAOQOC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_3832_cov_3.422822"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..358)
                     /locus_tag="PL106_03180"
     CDS             complement(32..358)
                     /locus_tag="PL106_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNA32100.1"
                     /translation="MRTWAVILVVIMAACGTAEESPVGLMPRDTFIEVLLQAHLIEAA
                     TADQAHADSAWAEMFAAQGVDRYRFRTTYAWYTEHPAELKAVYNEVLTGLQQRVDRPD
                     SAKVIH"
     gene            complement(355..1692)
                     /locus_tag="PL106_03185"
     CDS             complement(355..1692)
                     /locus_tag="PL106_03185"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNA32101.1"
                     /translation="MNGWLIRGARVVNEGRTYEADVLVRDDRIERLAPEGIGAVSGVN
                     EVDADGKYLLPGCIDDQVHFREPGLTHKDDITHGAVAAVAGGVTSFMEMPNTVPNTLT
                     QELLEEKYRIAARSSVANYSFFMGVSNTNMDEVLRTDPRTVCGLKAFLGSSTGDMLVN
                     EERSLDELFRKAHMILAVHAEDEPTIRANTAAAVARYGNNIPVEEHPYIRSAEACWLS
                     SSRAVARAKEHGTRLHVLHISTARELELFAPGPVEGKRITAEACVHHLWFTQEDHTRL
                     GTRIKWNPAVKTAVDREALRRAVAEGRIDVVATDHAPHTLGEKAEPYLRCPSGAPLVQ
                     HSLVAMLELTRQGVFSIEQVVERMSHMPARLFQVKDRGFIRAGAFADLVLVDLNDPWT
                     VGADNILYKCGWSPLEGQRFHARVRKTWVNGRLVHSDGVVDRTLRGERLLFDR"
     gene            complement(1689..2429)
                     /locus_tag="PL106_03190"
     CDS             complement(1689..2429)
                     /locus_tag="PL106_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNA32102.1"
                     /translation="MAERLVIIPTYNEQENIREILGHVFRLDPVFHVLVVDDGSPDGT
                     AGTVKELQAEYPDRLHLLERSGKLGLGTAYIMGFKWALERGYTYIFEMDADFSHDPDD
                     LVRLYEACAKDGADMSIGSRYVKGGHVRDWSWDRILLSWSASMYVRSILWLGVRDTTA
                     GFVCYHERVLRTLALDDIRFVGYAFQIEMKYRARLAGFRIAEVPITFVDRLKGTSKMS
                     ANIFREAVLGVWRMRFAVKQRRAEFALP"
     gene            complement(2519..>3832)
                     /locus_tag="PL106_03195"
     CDS             complement(2519..>3832)
                     /locus_tag="PL106_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32103.1"
                     /translation="AAPCPDASASINVELNAQPDAGQNVSILTCDQEAPFPLFAQLGG
                     NPDAGGTWTLPDGGTGNGQFIPGSSTSGNYTYTLLGVSPCVNDQATITIAVSEGVQAG
                     ANASISVCADADPFVLFPLLGSGADAGGTWTAPGGGSWNGSIDPSSGTSGVYVYTVVA
                     PAPCPVRMATVTVTINDVPSPLIMAEMADGCAPVEVTFSNANGDEGTYAWDFGNGQAG
                     VGGAPEPVLYGESGHFEVALTVTSPAGCIGGTVLVGGIDVYARPEAAFSHGPDRLNSN
                     APEAFFQNASTGANAYEWDFAGLGTSTETNPSFTFPDGVEGVYTICLTAFASENCFDT
                     ACAEVRVPLGAGLFVPDAFTPNGDGVNDVFTPIVNGVLEEGYRFMVFDRWGLEIFSTE
                     RPGTGWNGELDGMEAPVDVYVWKVTGRERYGTGHVDRMGHVTLMR"
BASE COUNT          713 a         1270 c         1131 g          718 t
ORIGIN      
        1 cggtggagaa taccgagcgg ttcctgcgcg gtcagtgaat caccttggcg ctgtccggcc
       61 gatccacccg ttgttgcagg ccggtgagta cctcgttgta cacggccttc aactcggcgg
      121 gatgctccgt gtaccatgcg taggtggtgc ggaaccgata acggtccacc ccctgagcgg
      181 cgaacatctc cgcccacgcg ctgtccgcat gcgcctgatc ggcggtggcg gcttcgatga
      241 ggtgtgcctg cagtaatacc tcgatgaatg tatcacgggg catcagccca acggggctct
      301 cctcggccgt tccgcacgcc gccatgatca ccaccaggat gacggcccac gttctcatcg
      361 gtcgaagagc agtcgttcac cgcgtagggt ccggtccacc acaccatccg aatgaacaag
      421 acggccattc acccaggtct tccgtacccg cgcatggaaa cgttgacctt ccaaaggcga
      481 ccaaccgcac ttgtacagga tgttgtccgc gccgaccgtc caagggtcgt tcaggtccac
      541 cagcaccaga tcggcgaacg ctcctgcgcg gatgaagcca cggtccttca cttggaacaa
      601 tcgtgcgggc atatggctca tgcgctccac cacctgctcg atgctgaaga caccctgacg
      661 ggtcagttcc agcatggcca ccagcgaatg ctgcacgagg ggtgccccgc tcgggcaacg
      721 caggtacggc tccgccttct caccgagcgt atgcggcgcg tgatcggtgg ccaccacatc
      781 gatgcggcct tcggccacgg cacggcggag cgcttcacga tccacggcgg tcttcaccgc
      841 cgggttccac ttgatccgtg tacccagcct ggtgtggtcc tcctgcgtga accagaggtg
      901 atgaacacac gcctccgccg tgatgcgctt cccttccacc ggtccaggtg cgaacagttc
      961 caactcacgc gccgtgctga tgtgcaggac gtgcaagcgt gtaccgtgtt ccttcgcccg
     1021 tgcgacggcc cggctgctgc tcagccagca cgcttccgcg ctccggatgt acggatgctc
     1081 ttcaacaggt atattatttc cgtatcgggc cacggctgcc gccgtgttcg cccggatggt
     1141 gggctcgtcc tcggcatgca cggccaggat catgtgcgcc ttccggaaca gctcatccaa
     1201 cgagcgctcc tcgttcacca gcatgtcgcc cgtactgctg cccaggaagg ccttcaaccc
     1261 gcagacggtg cgcgggtcgg tgcgcaacac ctcgtccatg ttcgtgttgg acacgcccat
     1321 gaagaaggaa tagttggcca cgctcgaccg cgcggcgata cgatactttt cttcaagaag
     1381 ttcttgcgtg agggtgttgg gcaccgtgtt cggcatctcc atgaagctgg tgacccctcc
     1441 ggccaccgct gccacagccc cgtgggtgat gtcatccttg tgcgtgaggc ccggctcgcg
     1501 gaagtggacc tggtcgtcga tacaacccgg taacaggtat tttccgtccg catcaacctc
     1561 gttcacgccg ctgacggctc cgatcccctc gggggccagt cgttcaatgc ggtcgtcgcg
     1621 caccagcaca tccgcctcgt atgtccggcc ctcgttcacc acccgggccc cgcgtatgag
     1681 ccagccgttc atggcagtgc gaactcggcc ctccgttgtt tcaccgcgaa tctcatgcgc
     1741 cacacaccga gcaccgcttc gcggaaaatg ttggcgctca tcttgctggt ccctttcaac
     1801 cggtccacga aggtgatggg tacttcggcg atgcggaagc cggccaggcg cgcgcggtat
     1861 ttcatctcga tctggaacgc gtagcccacg aagcggatat cgtccaatgc cagggtgcgc
     1921 agcacgcgct catggtagca cacgaaaccg gccgtggtgt cgcgaacgcc cagccaaagg
     1981 atggaacgca cgtacatgga ggcgctccaa ctgagcagga tgcgatccca cgaccagtcg
     2041 cgcacatggc cgcctttcac gtagcgcgac ccgatgctca tgtcggcacc gtccttcgca
     2101 caggcctcat aaagacgtac cagatcatcg gggtcgtggc tgaagtcggc gtccatctcg
     2161 aagatatacg tgtatccacg ctccaacgcc cacttgaaac ccatgatgta cgcggtaccg
     2221 aggccgagct tgccggagcg ctccagcagg tgaagacgat cggggtactc cgcctgaagc
     2281 tccttcacgg tgccggccgt accgtccggg gagccatcat ccaccacgag cacatggaac
     2341 acggggtcga gccggaacac atgaccgagg atctcccgga tgttctcctg ctcgttgtag
     2401 gtgggtatga tgaccagtcg ttccgccacg tgcgcgtgaa tgggtgccga agataaccgg
     2461 ggcggttccc cttcatggcc aataaggatc aaaggctgcg aaaagccttg aaaggcggtc
     2521 agcgcatgag ggtcacatgg cccatgcggt ccacatggcc cgtaccgtag cgctcacggc
     2581 ccgtgacctt ccacacgtat acatccaccg gcgcctccat cccatcgagt tcgccgttcc
     2641 accccgtacc cgggcgttcc gtgctgaaga tctccaaccc ccatcgatcg aataccatga
     2701 agcgataacc ttcttccagc accccgttca cgatgggggt gaagacatcg ttcacgccgt
     2761 ccccgttcgg tgtgaacgca tccggcacga agagtcccgc tcccagcggt acccgcacct
     2821 ccgcacaagc ggtatcaaag caattctccg atgcgaaggc cgtgaggcag atggtgtaca
     2881 ctccttccac accgtccggg aaggtgaagg acgggttcgt ctccgtggag gtgccgagtc
     2941 cggcgaaatc ccattcgtac gcattggccc cggtgctggc gttctggaag aaggcctcgg
     3001 gcgcattgct gttgagccga tcgggaccat ggctgaaggc agcttccggc cgcgcgtaca
     3061 catcgatccc gcccacgagc accgtaccgc cgatgcaacc ggccggggat gtgacggtca
     3121 gcgcgacctc aaagtgtccg ctctccccgt acagtaccgg ttccggagcc ccgcccaccc
     3181 cggcctgacc attgccgaaa tcccaggcat aggttccttc atccccgttc gcattgctga
     3241 aggtcacctc cactggggca cagccgtcgg ccatttcggc catgatcagt ggggagggaa
     3301 cgtcattgat cgtcaccgtg accgtggcca tgcgcacagg gcagggcgca ggagccacaa
     3361 cggtgtatac gtaaacaccg cttgtgccgc ttgatggatc gatacttccg ttccaggatc
     3421 caccccccgg cgctgtccag gtgccgccag catccgcacc gctgcccaag agggggaaca
     3481 ggacgaaggg gtccgcatcg gcacacacgg aaatactggc attcgcaccg gcctgtacac
     3541 cttcggaaac cgcgatcgtg atggtggcct ggtcattcac gcaaggtgaa acaccaagga
     3601 gcgtgtacgt gtagttgccc gatgtggagc tgcccggaat gaactggccg ttgccggtcc
     3661 cgccgtccgg tagtgtccac gtgccacccg catcggggtt gccgcccagc tgagcgaaca
     3721 gcgggaatgg agcttcctgg tcgcaggtga ggatcgacac gttctgaccc gcgtcgggtt
     3781 gtgcgttcaa ctccacgttg atcgaggcac tggcgtcggg gcagggtgcg gc
//
LOCUS       DAOQOC010000133         3830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11141_length_3830_cov_4.161854, whole genome shotgun sequence.
ACCESSION   DAOQOC010000133 DAOQOC010000000
VERSION     DAOQOC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_3830_cov_4.161854"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PL106_03200"
     CDS             complement(<1..656)
                     /locus_tag="PL106_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA32104.1"
                     /translation="MVRATGIRKRFGDLHVLQGVDLHVRKGEVVSIVGASGAGKTTLL
                     QILGTLEKADGGSLVINGEEVTRLGSDALSTFRNKHIGFVFQFHHLLPEFSALENVMM
                     PGLIAGRSVRTCTPRAEELLTRLNVLPRKDHKPGQLSGGEQQRVAVARALFNNPAVVL
                     ADEPSGNLDSAHARELHQLFFELRKELGQTFVIVTHNEELAAMADRRLVMKDGVIQGA
                     "
     gene            complement(689..1990)
                     /locus_tag="PL106_03205"
     CDS             complement(689..1990)
                     /locus_tag="PL106_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNA32105.1"
                     /translation="MSEGTTSMRYPDLIAPVHRALVEAGIPDGAATWLAMLTGIVIVF
                     TVMALLSKVLARLLFLTLKRMSARTESSFDDHLLRYRLHRYVARVVPLMASYNLVPIV
                     FQGMAGWIPIVERLFHVFFIVLFVRIARAVLRAARDNSRDRAAFRDKPLDSYVQVITL
                     IMYLVAGVLIFSQLTGQSALTFLTAMGAASAVLLLVFKDTIMGFVASIQISANDMVRV
                     DDWIEMPKYGADGDVTEINLTTVKVRNWDNTITTVPTYALIVDSFKNWRGMTESGGRR
                     IKRSILVKISSIRHLLPEELDHLRQVRLLRTYIDERVAEIARHNTEHHVDERLPINGR
                     RLTNVGLYRKYIEMYISAHPGIHKGMSLMVRQLAPNEHGLPLEIYCFANNTGWTEYEG
                     TMADIFDHLLAVNTFFHIEVFESPAADDVRMAMRSIAERKG"
     gene            2173..3363
                     /gene="sucC"
                     /locus_tag="PL106_03210"
     CDS             2173..3363
                     /gene="sucC"
                     /locus_tag="PL106_03210"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNA32106.1"
                     /translation="MNLHEYQGKIILQKFGVAVQRGLVAYNADEAVDQAKRLGQETGT
                     KWWVVKAQIHAGGRGKGGGVKLAKSYDEVREKSDAIIGMMLKTPQTPPQGKFVHKVLI
                     AEDVYYPGPSATKEYYMSVLLDRASARNVIVYSTEGGMDIEEVAEHHPDKIQKEFVDP
                     RVGLRPFQCHKIATALGCTGAAKKAMVKFIADLYRAYEGCDAAMFEINPVLKTSDDKV
                     IAVDAKVRLDGNALYRHPDFAEMRDKTEEDPTEVVAGEAGLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGEPANFLDVGGTADAARVEKAFRIILKDPSVKAILVNIFGGIVRC
                     DRVAQGIVDAYKNIGDIQVPIIVRLQGTNAKEAKDLIDNSGLKVLGAVALQEAADRVR
                     DVLK"
     gene            complement(3376..>3830)
                     /locus_tag="PL106_03215"
     CDS             complement(3376..>3830)
                     /locus_tag="PL106_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNA32107.1"
                     /translation="SFYTGIILLAGGINSGSDILAARTMGADLAYMGTRFIATRECLA
                     SDDYKQMILSSGIDDIIYTDGVSGVKANFIKQSLERAGIDLSQKKDEDFSKLSGSHAK
                     AWKDIWSAGHGSAGITDVPTVAELVMRLGIEYKAALERNDRIRNELAF"
BASE COUNT          725 a         1173 c         1199 g          733 t
ORIGIN      
        1 caggcccctt ggatcacccc gtccttcatc acgagccttc gatcggccat agcggccagt
       61 tcctcgttgt gcgtcacgat cacgaaggtc tgccccagtt ccttccgcaa ttcgaagaag
      121 agctggtgca gttccctggc atgcgccgaa tcgagattgc cgctgggctc atcggccagc
      181 accactgcgg ggttgttgaa gagcgcgcgc gccacagcca cgcgttgctg ctcgccaccg
      241 ctcagctggc ccggcttgtg gtccttgcgt ggcagcacgt tgaggcgggt caacagttct
      301 tcggcgcgcg gcgtgcatgt cctcacactt cgtccggcga tcaggcccgg catcatcacg
      361 ttctccaggg cgctgaattc cggcagcagg tgatggaact ggaacacgaa gccgatgtgc
      421 ttgttgcgga acgtactgag ggcatcgctg cccaaccggg tgacctcttc accattgatg
      481 accaacgagc cgccatcggc tttttccaag gtgcccagta tttgcaacag ggtggtcttg
      541 cccgcgccgc tggcgccaac gatactcacc acctcaccct tcctcacatg gaggtcgact
      601 ccttgcagca catgcaggtc gccgaatcgt ttgcggatac cggtggcgcg gaccatgggg
      661 acaaaggtgc gtgttggcgc cggatccttc agcccttccg ctccgcgatg gagcgcatgg
      721 ccatgcgcac gtcatcggcc gccgggcttt cgaagacctc gatgtggaag aaggtgttca
      781 ccgccagcag gtggtcgaag atgtcggcca tggtgccctc atattccgtc caaccggtat
      841 tgttggcgaa gcagtagatc tccagtggca ggccgtgctc gttgggggcc agctgccgca
      901 ccatgagcga catccccttg tggatgcccg gatgggcgct gatgtacatt tcgatgtatt
      961 tgcggtacaa ccccacgttg gtgaggcggc gcccgtttat cggcaggcgc tcgtccacat
     1021 ggtgctcggt gttgtggcgt gcgatctccg caacgcgctc gtcgatgtac gtccgcagca
     1081 accgcacctg gcgaaggtgg tccagttcct ccgggaggag gtgccggatg ctgctgatct
     1141 tgaccaggat gctccgcttg atcctgcgcc cgccgctctc ggtcatgccc cgccagttct
     1201 tgaacgagtc gacgatcagg gcgtaggtgg gcacggtggt gatcgtattg tcccagttgc
     1261 gcaccttcac ggtggtgagg ttgatctcgg tcacgtcgcc gtcggcaccg tacttgggca
     1321 tctcgatcca gtcgtccacg cgcaccatgt cgttggcgct gatctggatg ctggccacga
     1381 agcccatgat cgtatccttg aaaacaagta acaggacagc cgatgcggca cccatggccg
     1441 tgaggaaggt gagcgccgat tgaccggtca gctggctgaa gatgagcacc ccggcaacga
     1501 ggtacatgat cagtgtgatc acctgcacgt aactgtccag cggcttgtcg cggaacgccg
     1561 cccgatcgcg cgagttgtcc cgggccgcgc gcagcaccgc ccgggcgatg cgcacgaaca
     1621 gcacgatgaa gaacacatgg aacaggcgct ccacgatggg gatccacccg gccatgccct
     1681 ggaacacgat gggtaccagg ttgtagctgg ccatcagcgg caccacccgt gccacgtagc
     1741 ggtgcaggcg gtacctgagc aggtggtcgt cgaagctgct ctcggtcctg gccgacatcc
     1801 gcttcaacgt aaggaacagg agccgggcca ggactttgct gagcaaggcc atgaccgtga
     1861 acacgatgac gatgcccgtg agcatggcaa gccacgtggc cgcaccatcc ggaatgcccg
     1921 cctccaccag cgcacgatgc accggagcga tcaggtccgg atagcgcatg gaggttgtgc
     1981 cttcgctcat gatccctttc aactggagtc ggcgatcaag ggccggacag gtcggtaagt
     2041 tggcccttgg tacgaggcgc gaagctacgc cccgtccttc gggagtgcta tgcgatcgct
     2101 gctgcgtggt ggcgtgtaca ttcgcgccgc ccgcccgtcg gcggtcgttt tcccgatcga
     2161 agcaagccca ccatgaacct gcacgagtac caaggcaaga tcatccttca gaaattcggc
     2221 gtggccgtgc aacgcggcct tgtggcctac aatgcggatg aggctgttga ccaggccaag
     2281 cgcctcggcc aggagaccgg taccaaatgg tgggtggtga aggcgcagat ccatgcgggt
     2341 ggacgtggca aggggggcgg tgtgaaactg gccaagagct acgatgaggt gcgcgaaaag
     2401 agcgatgcca tcatcggcat gatgctcaag acgccacaaa caccgccaca ggggaagttt
     2461 gtgcacaagg tcctcatcgc ggaggatgtg tactaccccg gtcccagtgc gaccaaggag
     2521 tattacatga gcgtgctgct ggaccgggcc agtgcccgga acgtcatcgt gtacagcacc
     2581 gaaggcggca tggacatcga ggaggtggcc gagcaccatc cggacaagat ccagaaagag
     2641 ttcgtggacc ctcgtgtggg cctgcgcccg ttccagtgcc acaagatcgc tacggccttg
     2701 ggctgcaccg gtgccgccaa gaaggccatg gtcaagttca tcgccgatct gtatcgcgcc
     2761 tacgagggtt gtgatgcagc gatgttcgag atcaaccccg tgctcaagac cagcgacgac
     2821 aaggtgatcg ccgtggatgc caaggtgcgc cttgatggca acgcactcta ccgccatccc
     2881 gacttcgcgg agatgcgcga caagaccgag gaggatccta ccgaggtggt cgcaggggag
     2941 gccggcctca attacgtcaa actcgatggc aacgtgggtt gcatggtgaa cggtgccggc
     3001 cttgccatgg ccaccatgga catcatcaaa ctcagcggcg gtgaaccggc gaacttcctg
     3061 gatgtgggag gcaccgccga cgccgctcgc gtggaaaagg ccttccgcat catcctgaag
     3121 gatcccagcg tgaaagccat cctggtgaac atcttcgggg gtatcgtgcg ttgcgaccgc
     3181 gtggcccaag gcatcgtgga cgcgtacaag aacatcggcg atatccaggt gcccatcatc
     3241 gtgcgtctgc aagggacgaa tgccaaggag gccaaggacc tcatcgacaa cagcgggttg
     3301 aaggtgctgg gtgccgtggc gctgcaagag gccgccgacc gggtgcgcga cgtgctgaag
     3361 tgatcggcct tgggatcaaa acgccagttc gttcctgatc cggtcgttcc gttccagcgc
     3421 cgctttgtat tcgatcccca accgcatcac aagttcggcc acggtgggca catcggtgat
     3481 gcccgcgctg ccgtgccccg cgctccagat gtccttccac gccttggcat gggacccgct
     3541 cagcttggag aagtcctcgt ccttcttctg cgacaggtcg atccccgcac gttccaggct
     3601 ctgcttgatg aagttcgctt tcacccccga tacgccgtcg gtgtatatga tgtcgtcgat
     3661 ccccgatgac aggatcattt gtttgtagtc atcgctggcc aggcattccc ttgtggcgat
     3721 gaaacgcgtg cccatgtacg cgaggtccgc gcccatggtc cgcgccgcca ggatgtcgct
     3781 ccccgaattg atgccgccag ccagcaggat gatccctgtg tagaagctcc
//
LOCUS       DAOQOC010000134         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11281_length_3799_cov_3.359509, whole genome shotgun sequence.
ACCESSION   DAOQOC010000134 DAOQOC010000000
VERSION     DAOQOC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11281_length_3799_cov_3.359509"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            565..>3799
                     /locus_tag="PL106_03220"
     CDS             565..>3799
                     /locus_tag="PL106_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32108.1"
                     /translation="MWDAGMEHYNNEDLPADLNVPQTASGRDWYNDVCLVRDAVGAHV
                     GYAVCGYSYIPNYFNQNGDCPPGNTNTGYPNPTYLETFEQRWGTITGTVACYDLAGNR
                     LWMHTYLVGSFDGIIQDKNGDLVVAGFSEQMAPWPNMPGTAQPFYYNPTVSQQEHYDV
                     AEGGCDPDTRARKMVLMKVDRTDGHALWNHYYGPADDPVVGMPMRTQGSSVAEVDPDG
                     VGGYRVVGYCKVPIGDQQVIRDRPFLVDVDVNGMLNWKQVMDSTHPSGLWPDSNLGSQ
                     AFRIVRHPDPAQDKYLITGVRYPTGIFSAFLLYYNDNGSTPAWWADTDIDEPDFDLSE
                     NSACSSNRACFAVSGGTTSVIWPVLSDHIGAWWGSADHTAVGRVYKLDLNGDLVWNDP
                     VNLGWMRGFDHWFSANQRANGNIILGCTKSIQSASNPLPFNSPPPAISLPLAVQNCLQ
                     TTFGYDPVPPLGTAPDPFDWTQAGISDWGYWLSDSYMAEVDLDDGSVVWEGTWDADAV
                     DGVTPVCNPDNIRQRQCSFNVIEADDGGLVSCGNTGKNNEDGYLIKFNGCESDPTLYA
                     TFHASHPYDPSNVYTLTANETWGSSMNVRGSVVVPAGKTLLINNNAVISFADSRRIGY
                     TTNIVVQPGGALTVTGGARLTNIGPCGNGMWDGIKALGNTNNPNQNSTHQSTIQISGD
                     AIIENAFVAVLAANADINDPVGSVATDRGARVFGVNATFRNNIYDVVLRPYNTAGTPV
                     TNFQECDFLTTGPLNYPDRTPKTHLYVNAYPRLNIHGCTFENTSPSLDQNQVAAWGKG
                     IEGFNTDLRILPLNGRDPHFTSLEGGCFAANANAKPVFVDGAFFTGCGHGLGIVASGN
                     ARVVNCEFKEPDLDMVGQGLGAPVYGSYLDGTTTILFEHNSFWAAVNDLQPPLYGNPL
                     VGSTFKDLGGNSNRFFDNSYYGFKGTNGVLYSAGATIQGVNDGQGITDGLQFKCNQFS
                     YLGPGAGDDDFDLAFTGPNVSVGGVQGSGLDPTAPAGNTFLMSCTGEAHMKLDDVPNN
                     NGLYFQYWHHAPTSGVEVVPTCLTNPPLIPSGLGTINQPTTFLFDRSQ"
BASE COUNT          772 a         1174 c         1103 g          750 t
ORIGIN      
        1 gcctgcatcg ccttcgcgta gccgcttcgg cgcagcaagg cgccagagcg cttcggcgtc
       61 ggcgcgcaag gcgaggagtg cttccaaccc ccttgcaggg tctcatcagg atcgttcagc
      121 cccgtttcgt gctccgcgct tcgcagcggg gcggtcggcg cgctcagcgt gacacctcta
      181 attgatcatc ctgcgcaatt gggatgcggg tctcttgtat ggatcgtccg ttccctgtcc
      241 catccccacc gttccctgcc cttcgcaccg atcgttcata aaaattcctt gcccgttcca
      301 ttcttcttcc ccttcttcgc tactgctcca ctaacaaaaa tccagtgaac cgatgaaggc
      361 gttccggaca agtgaaaagc agggggtgga tcccgctgcc aaagaagggg gagaaggccc
      421 gcgaaaccgt gctggacggg cgaaaatgaa aatggggggg taattctcct cggtattatt
      481 tctgcgcctc tcatggccca gttgccatcc gttgagtggc tcaggcaaca cgccctcccg
      541 gaacgccctc cgggcctgag cttcgtgtgg gatgcaggta tggagcatta taataacgag
      601 gatctgcccg cggatctcaa cgtgccgcaa acggcttccg gtcgcgattg gtacaacgac
      661 gtttgtctgg tgcgggatgc ggtaggtgca catgtcggtt acgcggtgtg cggatattca
      721 tacattccca actacttcaa ccagaatggg gactgtccac caggaaatac gaacaccggg
      781 tacccgaacc ccacgtactt ggaaacattc gaacagcgct ggggcaccat tacgggtacg
      841 gtggcctgct acgacctcgc tggcaaccgc ctatggatgc acacctatct ggtgggctcc
      901 ttcgacggga tcattcagga caagaatggt gatctggtgg ttgccggatt cagtgagcag
      961 atggccccat ggcccaacat gccgggtacc gcgcaacctt tttactacaa ccctaccgta
     1021 agtcaacaag agcactacga tgtggcggaa ggtggctgtg atccggatac cagggcccgc
     1081 aagatggtgc tgatgaaagt ggaccgcacc gatggtcatg ccctttggaa ccattattac
     1141 ggcccagcgg acgatcctgt tgtgggtatg cccatgcgca cccagggatc ttcggtggcc
     1201 gaggtggacc ccgatggtgt gggtggctac cgggtcgttg gctattgcaa ggtcccgatc
     1261 ggcgaccagc aggtcatcag ggatcgtccc ttcttggtgg atgtggatgt gaacggcatg
     1321 ctgaattgga aacaggtgat ggacagcacg catccctccg ggctctggcc cgatagcaac
     1381 ctgggcagcc aggcctttcg catcgtcagg caccctgatc cagcgcagga caagtacctg
     1441 atcaccggag tgcgctatcc gaccggcatt ttctccgctt tcttgctgta ttacaacgat
     1501 aatgggtcga caccggcatg gtgggcggat acggacatcg atgaaccgga tttcgacctg
     1561 agcgagaact cggcatgtag ctccaatcgc gcgtgcttcg ccgtgagcgg cggcaccaca
     1621 tcggttatct ggcccgtgct ctccgatcac atcggggcgt ggtggggctc ggccgaccat
     1681 accgctgtgg ggcgtgtgta caagttggat cttaacgggg acctggtctg gaacgatccg
     1741 gtcaacttgg gctggatgcg cggcttcgat cactggttct ccgccaacca acgcgccaac
     1801 ggcaacatca tccttggttg cacgaagagc atccaaagcg cctcgaaccc cctgcccttc
     1861 aattcccccc cgcctgctat ttcgcttccg cttgccgtgc agaattgcct gcaaaccacc
     1921 ttcggttacg atcccgtccc gccgcttggt accgcacccg atccgttcga ttggacccag
     1981 gcagggatct cggactgggg atattggctc agcgacagct atatggctga ggttgacctg
     2041 gacgacggtt cggtcgtttg ggagggtaca tgggatgcgg atgccgtcga cggagtaacg
     2101 ccagtctgca accccgacaa catccggcag cggcaatgct cgttcaatgt gatcgaggcc
     2161 gatgatggcg gcttggtgag ttgtggcaac accggcaaga acaacgagga cggctacctc
     2221 atcaaattca acggctgcga aagcgatccc acgctctacg ccacattcca cgcctcgcac
     2281 ccctacgacc cctccaacgt gtacaccctc acagccaacg aaacctgggg ttccagtatg
     2341 aacgtgcggg gtagcgtcgt ggttcctgcg gggaaaacac tcctcatcaa caacaacgcc
     2401 gtgatctcct tcgccgatag ccgccgcatc gggtacacca ccaacatcgt ggtgcagccg
     2461 ggcggtgcac tcacagtgac cggcggcgca cgcctcacca acatcgggcc ctgtggcaac
     2521 ggcatgtggg acggcatcaa ggccttgggt aacacgaaca atcccaacca gaacagcacg
     2581 caccagtcca ccatccagat cagcggcgat gccattatcg agaacgcctt cgtggccgtg
     2641 ctggcggcca atgcggacat caatgatcct gtgggcagtg tcgccaccga ccgtggtgcg
     2701 cgggtgttcg gtgtgaacgc caccttccgg aacaacatct acgacgtggt gctgcggcca
     2761 tacaacaccg cgggcacgcc cgttaccaat ttccaggagt gcgacttcct caccaccggc
     2821 ccgctgaact acccggacag gaccccgaag acgcacctct acgtgaacgc ctacccgcgc
     2881 ctgaacatcc acggttgcac cttcgagaac acctcgcctt cgctcgacca gaaccaggtg
     2941 gccgcatggg gcaagggcat cgagggcttc aataccgatc tgcggatcct accgctcaac
     3001 ggccgcgatc cacatttcac cagtctggag ggtggctgct tcgcggccaa cgccaatgcc
     3061 aagccggtgt tcgtggacgg tgccttcttc accggctgtg gccacggcct gggcatcgtg
     3121 gcctcgggca atgcgcgggt cgtcaactgc gagttcaaag aacccgacct ggacatggtg
     3181 ggccagggct tgggtgcgcc cgtgtacggc agctacctgg atggcacgac cacgatcctc
     3241 ttcgagcaca actcattttg ggcggccgtg aatgacctgc aaccaccact gtatggcaac
     3301 ccgctggtgg gcagcacctt caaggacctc gggggtaaca gcaaccgctt cttcgacaac
     3361 tcctactacg ggttcaaggg taccaatggg gtgctctaca gcgcgggtgc taccatccaa
     3421 ggggtcaacg atggtcaggg tatcaccgat gggctccagt tcaagtgcaa ccagttcagc
     3481 tacctggggc cgggggctgg ggatgatgat ttcgacctgg ccttcactgg gccgaacgta
     3541 agcgtggggg gagtgcaagg gtcaggtctc gatcccaccg cacccgctgg taacaccttc
     3601 ctgatgagtt gcaccggtga agcgcacatg aagttggacg atgtgcccaa caacaacggg
     3661 ctctacttcc agtactggca ccatgcgccc acatcagggg tggaagtagt tcccacctgc
     3721 ctcaccaacc cgccgttgat cccgagcggc ttgggtacga tcaatcagcc cacgacgttc
     3781 ctcttcgata ggagccagg
//
LOCUS       DAOQOC010000135         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11356_length_3787_cov_3.128617, whole genome shotgun sequence.
ACCESSION   DAOQOC010000135 DAOQOC010000000
VERSION     DAOQOC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11356_length_3787_cov_3.128617"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PL106_03225"
     CDS             <1..244
                     /locus_tag="PL106_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32109.1"
                     /translation="NQRDRIASYSMDDRVKMLRLRPDRADVVVPAADIFLDILGHAGI
                     DEVFVPKVGLADGIVYDLYMRKRGHVRYPHVEVVGA"
     gene            complement(295..978)
                     /locus_tag="PL106_03230"
     CDS             complement(295..978)
                     /locus_tag="PL106_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNA32110.1"
                     /translation="MCYNTTLDRDVRTLEKRMHKRMLEEGRRVGDPDPPELPLDRTTA
                     FARPLWPVVSSRSPDRISVQRWGLLPRYIRTDEEAREFLKRTPTFNAISEEVAAKRTF
                     KRAFEAGQRCLVPVTAFQEWRHEGKEKIPYRITMKDGDVFCLGGLWEEHTDGDTYTVL
                     TTRANPLMEFVHNTRKRMPVIIAEEHWGTWLLPGLPASEVLGMCAPYPEELMLAEPVG
                     QRPVQASLF"
     gene            1153..2661
                     /locus_tag="PL106_03235"
     CDS             1153..2661
                     /locus_tag="PL106_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNA32111.1"
                     /translation="MRTTASLLLVLSIRLHGATYHVAPGGSDTDDGLTLATAFATIQH
                     GADVAVAGDSVFVHPGGYTGFAAMDNSGTAGAPIVFHATDQGVNITSPCAYNNLDGIN
                     IENVAWVTVEGFTVNDMPRTGIRTALTDHVTLRDNSCNDNYKWGILCGFAEHVIIEYN
                     TCSGSEDEHGIYFSNSADDPIIRYNHCFNNNANGIHMNGDVSMGGDGVISNAQVYGNI
                     IHGNGEAGGSGINCDGVANSAIFNNLLYDNHASGISLYQIDGGAPSTGNKVYNNTIIN
                     ASDARWCVNITEGCTGNQVLNNILINQHPFRGSITIAADALDGFVSDHNLVTDRLSSD
                     GDATILDLPAWQDMGYDTHSQVADAPTALFVDPLVNDFHATGANAQMVDAGTSAVSSV
                     VNNDLEGQVRPLGSAFDIGCYEADPSTSIAPAHPSDDAQLIWNFRGTICFTPLPEAVV
                     LDCYDASGRLLSAHRLSPETMQVPAPSASWFLVVLRDVHGRVVSTQRLTGVE"
     gene            2919..>3787
                     /locus_tag="PL106_03240"
     CDS             2919..>3787
                     /locus_tag="PL106_03240"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA32112.1"
                     /translation="MSTRTYLYLLLFFLAQACAPPAPEHAAHPSTADPTVARIDTFLS
                     AATAAGFSGSVLVMRGDSILLDKGYGMRDREQQLPNTPGTVHAIGSITKQFTAACILK
                     LQESGKLNVSDPMDNYLPGVPADKAGITLHQLLTHRAGFPGAIGDDNEAIDGGAFTKL
                     AMATPLEFKPGTAYAYSNVGYSLLGLIVEQVSGMGYERYLHDSLLVPSGVLHTGYQLP
                     DWSKEELAIGYRKDGTRWGTMLDHPLVNGGPGWHLRANGGLLSTTHDMAAWINALRTH
                     TVLSATSTNAMFTP"
BASE COUNT          726 a         1268 c         1100 g          693 t
ORIGIN      
        1 caaccaacgg gatcgtatcg catcctacag catggacgac cgggtgaaga tgctccggtt
       61 gcggcccgac cgcgccgatg tggtggtgcc cgccgccgac atcttcctcg acatcctcgg
      121 tcatgcgggt atcgacgagg tgttcgtgcc caaagtgggc ctggccgatg gaatcgtgta
      181 cgacctgtac atgcggaaac gcgggcatgt gcgctatccc cacgtggaag tggtcggtgc
      241 gtgatcgacc gttcccgggt tgtcctgtgc cccttcgcca cagaaggtcg ccattcagaa
      301 aagcgaggcc tgcacaggtc gctggcccac gggttccgcc agcatcaact cctcgggata
      361 cggcgcgcac atccccagca cctcggaggc ggggagcccg ggcaggagcc aggtacccca
      421 atgttcctcg gcgatgatca ccggcatgcg cttcctggtg ttgtgtacga actccatcag
      481 tggattggcc cgcgtggtga gcacggtgta cgtgtccccg tccgtgtgct cctcccacag
      541 tcctcccagg cagaacacgt ccccgtcctt catcgtgatc cggtagggta ttttttcctt
      601 gccctcgtgc cgccattcct ggaaggccgt taccgggacc agacaacgct gaccggcttc
      661 gaacgcacgt ttgaacgtgc gcttcgcggc cacctcctcg ctgatggcgt tgaacgtggg
      721 cgtgcgcttc aggaactccc tggcctcctc atccgtacgg atgtaccggg gcagcaatcc
      781 ccagcgctgc acactgatcc ggtcgggcga tctgctgctc accaccggcc atagtggtcg
      841 ggcgaacgcc gtagtgcgat ccagtggcag ttcaggcgga tccggatcgc ccacccgccg
      901 tccctcctcc agcatccgct tgtgcatgcg cttctccagc gttctcacat cgcggtccag
      961 cgtggtgttg tagcacatgg gacaaaggaa cgcctcccac cgcaccccgt gcgagcggct
     1021 ttctggccga cctgcggccc cgtccatgtc cggaagcagg atcccgacga gcgatgcccg
     1081 ccaccggtcg cagcatgtac cgtcccggaa ggatcatccg aaccatcgcc ccgtatcttc
     1141 gttgggttta ccatgagaac gaccgcctcc ctccttcttg tcctgtccat caggcttcac
     1201 ggtgcaacgt accatgtggc accgggcgga agcgacaccg atgatggcct gaccctggcc
     1261 accgcgttcg ccaccatcca gcacggggcc gatgtggccg tggcgggcga cagtgtgttc
     1321 gttcatccgg gcggttacac gggcttcgcg gccatggaca acagcggtac cgctggagca
     1381 cccatcgtct tccacgccac cgaccagggg gtcaacatca catcaccgtg cgcctacaac
     1441 aacctggacg gtatcaatat cgagaatgtg gcgtgggtga ccgttgaagg cttcactgtg
     1501 aacgatatgc cgcgcaccgg catccgcacg gccctcaccg accatgtgac cctgcgggac
     1561 aacagctgca acgacaatta caaatggggc atcctgtgcg gcttcgcgga gcatgtgatc
     1621 attgaataca atacgtgcag cggtagcgag gatgaacatg ggatctactt cagcaacagt
     1681 gccgatgacc cgatcatccg ctacaaccac tgcttcaaca acaacgccaa cggcatccac
     1741 atgaacggcg atgtgagcat gggcggcgac ggcgtgatca gcaatgcgca ggtgtacggc
     1801 aacatcatcc acggcaacgg cgaggccggt ggcagcggga tcaattgcga cggtgtggcg
     1861 aactccgcca tcttcaataa tctactgtac gacaaccacg ccagcgggat cagcctctat
     1921 cagatcgatg gcggcgcgcc cagcacgggc aacaaggtgt acaacaacac catcatcaat
     1981 gccagcgatg cgcgctggtg cgtgaacatc accgaaggat gtacaggcaa ccaggtactg
     2041 aacaacatcc tgatcaacca gcacccgttc cggggcagca tcacgatcgc cgccgatgcg
     2101 ctggatggct tcgtcagcga tcacaacctc gtgacggatc ggctttcgtc cgatggcgat
     2161 gcaacgatcc ttgatctccc cgcgtggcag gacatggggt acgataccca ttcccaggtg
     2221 gccgatgcgc caaccgcact gttcgtggat ccgttggtca acgacttcca cgccacgggc
     2281 gccaacgccc agatggtgga tgccggtacc tctgctgttt caagtgtggt caacaacgac
     2341 ctggagggcc aggtcagacc acttgggtcg gcgttcgata tcggctgcta tgaggccgat
     2401 cccagcactt ccatcgcacc cgcgcatcct tccgatgatg cccaactaat ctggaacttc
     2461 cgcggcacga tatgtttcac accccttccc gaggctgtag tcctagactg ctacgatgcg
     2521 tccgggcgat tgctgtcggc ccaccggctg agcccggaaa ccatgcaggt ccctgcaccc
     2581 tctgcgtcct ggttcctcgt ggtgctgcgc gatgtgcatg gccgggtggt gagcacccag
     2641 cggctcacgg gtgtggaata agctccaaga cctgcaagga tcggcggggc atgggggtga
     2701 taggacaccg gacctgctcc gctccgccaa cgcgggatcc gtgccattca attgccaacc
     2761 gtggttcatt gggatcgaac cggcgtactt ccggacaagc catcgccagc ctcacggtgt
     2821 gcaatgctgc gaacgttccg tccctcggat cacaaacgtt aacgcgctcc ctctcgccct
     2881 gcccagtgca ccataggcgg gcgctctacc ttgtgttcat gtccacccgc acgtacttgt
     2941 acctcctgct cttcttcctg gcccaagcct gtgcgccgcc cgcgccggag catgccgcgc
     3001 atccatctac tgcggaccct accgtcgccc gcatcgatac cttcctctcg gccgcaacgg
     3061 cggcggggtt cagcgggagc gtgctggtga tgcgcggcga tagcatcctg ttggacaagg
     3121 gctatggaat gcgcgaccgt gaacagcaac tgccgaacac cccaggcacc gtgcatgcca
     3181 tcggctccat caccaagcag ttcaccgcag cgtgcatcct gaagttgcag gaaagcggca
     3241 aactgaacgt ttcggacccc atggacaact acctgccggg cgtaccggcg gacaaggccg
     3301 ggatcacctt gcaccaattg ctcacccaca gagcggggtt ccccggggcc atcggcgacg
     3361 acaacgaggc catcgacggc ggagcgttca cgaagctcgc catggccacg ccgttggaat
     3421 tcaagcccgg caccgcttac gcgtacagca atgtgggcta cagcctgctg ggcctcatcg
     3481 tggaacaggt gagcggcatg ggctacgaac gctacctgca cgactccctg ctggtgccct
     3541 caggcgtgct gcataccggc taccagttgc ccgactggtc caaggaagag ctcgccatcg
     3601 gctaccgcaa ggacggtacg cgctggggca ccatgctgga ccatcccctg gtgaacggtg
     3661 gtcccggctg gcatctgcgc gccaacggcg ggctgctcag caccacccac gacatggccg
     3721 cgtggatcaa cgccctgcgg acccacacgg tgctctcagc gaccagcacc aacgccatgt
     3781 tcacccc
//
LOCUS       DAOQOC010000136         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11384_length_3782_cov_4.231285, whole genome shotgun sequence.
ACCESSION   DAOQOC010000136 DAOQOC010000000
VERSION     DAOQOC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11384_length_3782_cov_4.231285"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..338
                     /locus_tag="PL106_03245"
     CDS             60..338
                     /locus_tag="PL106_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32113.1"
                     /translation="MLTSVLIDVHLTDMACGYKLMTRDLFEKLNLKEDRFGFEAELII
                     KCGRMRRNWITEVPVHYYPRNAGEGKKLRNADGLRIMRTIIKYGLFRL"
     gene            338..1705
                     /locus_tag="PL106_03250"
     CDS             338..1705
                     /locus_tag="PL106_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32114.1"
                     /translation="MAISTAKGLPRLVIALVVAGCAWVDLDLKLWQLRDRVIEWDAHS
                     YYAYLPAQLIYDDIRLIKSDYRFDEDYFLFWPSSAENGGRVIKTTMGVAVCYAPFFLL
                     GHLYASLSAYPADGWSPPYKIALLIGALFYLAMGLELTRHLLARCGFRATTTAITLAL
                     IGSGTNLFCYASQSATSSHVYSFFLVAWSMNLVLSWYERPSFRRAAGLGALLGLVSLV
                     RPTNVLLWCVFALYGIVGYKTFMSRLIFLFRRWAQLLVMAIAAFIVWVPQLMYWKTIT
                     GAYFYNSYIGEHFFFTSPHILEGMFGFRKGWLIYTPIMAFALAGLGLLRDRAASLRWG
                     LIVLMVLSIYVTFSWWCWWYGGTFGQRAMIDLYPLLAIPLAAFVDRVLQGRLWVRIVA
                     TGAGSFLVWLNIFQTLQFEEGSLHYDAMTFRCYVKQFGRMEKVEDFEQLLDHPDYDRA
                     RQTGQ"
     gene            complement(1742..2146)
                     /locus_tag="PL106_03255"
     CDS             complement(1742..2146)
                     /locus_tag="PL106_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32115.1"
                     /translation="MKKDILSPKVEGVCMAVVREADEEGEPAWYVYLINEKDVPLNTV
                     LISSSGQGEIDGEQRATSEMRHYLETLGPKSWARIERIVEDVFALSNQYWLSFYIGGD
                     IHDKRYVFVAGSINEENFTRLPLMGTKGVLIG"
     gene            complement(2211..3377)
                     /locus_tag="PL106_03260"
     CDS             complement(2211..3377)
                     /locus_tag="PL106_03260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA32116.1"
                     /translation="MRFLRKLLLWLVGITALFVILAYATGNGHLVRGVRYTYLIGRTS
                     PEIDDRNFFPYTTIPATAPQPWPRSSRYGKLTLSAEQEQRLKDLYSVGFAVFQHDSLL
                     FEGYWNGWDADSVSNSFSVAKSYISVLTGVAIGEGRINSVKQPVGDFLPQFKEGCHAN
                     ITLENVLTMSTGLDWSESGADPFSDNAKGYYGSDVRELSLDQPCRDEPGKEFDYISGS
                     TQIMAEVLEAAYGRPLDELVREKIWTPLGCEHDAYWGKDREDGDFKAFCCLYATARDF
                     GRIGQLYLDSGVWKGTRIVPLDYWKASITPAPLLDKGAPNKRYGYFWWLAELDGHPIH
                     YCRGFHGEYVVVIPHEDLVLVRTGMRREEVNATGHPTDVFEWIAIARELAARPV"
     gene            complement(3407..>3782)
                     /gene="lipB"
                     /locus_tag="PL106_03265"
     CDS             complement(3407..>3782)
                     /gene="lipB"
                     /locus_tag="PL106_03265"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNA32117.1"
                     /translation="QHFTDIHRYLRTLEEAIIGTLETYGIKAGRITGQTGVWIDEAEP
                     LKARKICAFGIRASRWVTMHGFAFNVNTDLAYFGHIIPCGIADKGVTSLAKELGREVD
                     MGEVKDRLKMELANLFDVELVG"
BASE COUNT          668 a         1128 c         1122 g          864 t
ORIGIN      
        1 caggttgaca gtgaagtgct ccgaaatcgc caactgcggc aacacgcaaa ccgttctcct
       61 tgctcacgtc ggtgctcatc gatgttcatc tcaccgacat ggcgtgtggc tacaagctca
      121 tgaccaggga tctctttgag aagttgaacc tgaaggagga tcggttcggg ttcgaggcgg
      181 agttgatcat caagtgcgga cgtatgcggc gcaattggat caccgaagtg cccgtgcatt
      241 actacccgcg aaacgcgggc gaggggaaaa agcttcgcaa tgcggatggt ttgcgcatca
      301 tgcgcacgat catcaaatac ggtctgttcc ggctgtgatg gccatctcga cagccaaggg
      361 attgccacga ctggtcatcg cattggtggt ggcagggtgc gcatgggtgg atcttgacct
      421 caagctctgg cagctacgtg accgtgtgat cgagtgggac gcccactcct actacgcata
      481 cctgcctgcg cagctcatct atgacgacat ccgtctcatc aagagcgact atcgcttcga
      541 tgaggactac ttcctcttct ggcccagttc ggcggagaac ggtggtcgtg tgatcaagac
      601 caccatgggc gtggctgtat gttacgcgcc cttcttcctc ctgggtcacc tctacgccag
      661 cctctcggcc tacccggcgg atggttggtc gccgccctac aagatcgcac tgctcatcgg
      721 ggcgttgttc tacctggcga tggggctcga actgacccgg cacctgttgg cgcgctgcgg
      781 tttcagagcc accaccacgg ccatcacgtt ggcgctcatc ggttctggta ccaacctgtt
      841 ctgttacgcc tcccagtcgg ccacgagctc acatgtgtac tcgttctttc tcgtggcctg
      901 gtccatgaac ctcgtgctat cgtggtacga aaggccgagc tttcggcgtg cggctgggtt
      961 gggcgccctg ctgggacttg tttccctggt gcgccctacg aatgttttgt tgtggtgcgt
     1021 ctttgcgctg tatggaattg ttggatacaa gacgttcatg agcaggctga tcttcttgtt
     1081 ccgcaggtgg gcccaattgc tggtgatggc tatcgctgcc tttatcgtat gggtgccgca
     1141 actcatgtat tggaagacca tcactggtgc ctatttctac aactcgtaca tcggtgagca
     1201 tttcttcttc acttccccgc atattctgga gggcatgttc ggcttccgga aagggtggct
     1261 gatctacaca cccatcatgg cgttcgcttt ggcggggctg ggtcttttgc gtgaccgtgc
     1321 ggcttcgctg cgatggggac tcatcgtact catggtcctg agcatctacg tgaccttttc
     1381 ctggtggtgc tggtggtacg gcggcacgtt cggtcagcgc gccatgatcg atctgtatcc
     1441 attgctggct atcccattgg ccgcattcgt tgaccgtgtg cttcagggcc gcttgtgggt
     1501 acggatcgtg gccaccggcg ccggttcctt cctggtctgg ctgaacattt tccaaacatt
     1561 gcagttcgaa gaaggatccc tgcactacga tgcgatgacc ttcaggtgct acgtgaagca
     1621 gttcggccgg atggagaagg tggaggactt cgagcaatta cttgatcatc cggactacga
     1681 tcgcgcccgc cagacaggtc agtaggcgga ccagggcgat cgtgggatcg ccatccacca
     1741 ttcagccgat cagcaccccc ttcgtcccca tcagcggcag ccgggtgaag ttctcctcgt
     1801 tgatgctccc ggccacgaat acataccttt tgtcgtggat gtcaccaccg atgtagaagc
     1861 tgagccagta ctggttgctc aaggcgaaaa cgtcctccac gatgcgttcg atgcgggccc
     1921 agctcttggg gcccagggtc tccaagtagt gacgcatctc actggtggcg cgctgttcgc
     1981 cgtcgatctc gccctggcca ctggagctga tgagcacggt attgagcggc acgtccttct
     2041 cgttgatcag gtacacgtac caggcgggtt cgccttcctc atcggcttcg cgcaccacgg
     2101 ccatgcacac gccttccacc ttgggggaga ggatatcctt cttcatcgga gcgtgcaagg
     2161 tacgtgtgcg accatgccgg cgcggggatc aacgcccacc tgacctccgt tcaaacagga
     2221 cgtgcggcga gttcccgcgc tatggcgatc cactcgaaga catccgtggg gtgtccggtc
     2281 gcgttcactt cctcgcgcct catgccggta cgcacgagca ccaggtcctc gtgggggatc
     2341 accaccacgt actcgccatg gaagcccctg caataatgga tcgggtgccc gtccagctcg
     2401 gccagccacc agaagtaacc gtaccgcttg ttcggggcgc ccttgtcgag caggggcgcc
     2461 ggggtgatgc tggctttcca gtagtcgagc ggaacgatac gcgtgccctt ccaaacaccg
     2521 ctgtccaggt ataattgtcc gatgcgcccg aaatcgcggg cggtggcgta caggcaacag
     2581 aaggccttga agtcgccatc ctcccggtcc ttgccccagt aagcatcatg ttcacagccc
     2641 aggggcgtcc agatcttctc gcgtaccagc tcatccaatg gtcggccgta cgcggcctcc
     2701 agcacttcgg ccatgatctg tgtgctcccg ctgatgtagt cgaactcctt gccgggttcg
     2761 tcgcggcagg gctggtccag actcagctcg cggacatcgc tgccgtaata gcccttggcg
     2821 ttgtcgctga acgggtcggc accgctttcg ctccaatcca gccccgtgct catggtgagc
     2881 acgttctcaa gggtgatgtt cgcatggcat ccttccttga actgagggag aaaatcgccc
     2941 accggctgtt tcacgctgtt gatccgcccc tcgccgatgg ccacaccggt tagcacgctg
     3001 atgtagctct tggccacact gaaactgttg ctcacgctgt cggcatccca gccgttccaa
     3061 tacccttcaa aaagcaggct gtcgtgctgg aagacggcga agcccacact gtacaggtcc
     3121 ttcaagcgtt gttcctgttc cgcggagagg gtcagcttgc catagcgcga gctgcgtggc
     3181 catggttggg gcgccgtggc cggaatggtg gtgtacggaa agaagttccg gtcgtctatc
     3241 tcgggcgatg tgcggccgat gaggtacgtg taacgcacgc cacgcaccag atggccgttg
     3301 cctgtggcgt aggcgagtat cacgaagagg gcggtgatcc cgacgagcca gaggaggagt
     3361 ttgcggagga aacgcatcgg gcgaatgtag tcggcggttc ggggcttcag cccaccagtt
     3421 ccacatcgaa gaggttggcc agctccatct tcagccggtc cttcacctcg cccatgtcca
     3481 cttcgcggcc cagctctttc gccaggctcg tcacaccctt gtccgcaatg ccgcacggaa
     3541 tgatgtgccc gaagtaggca aggtccgtgt tcacattgaa tgcgaagcca tgcatggtga
     3601 cccagcggct ggcgcggatg ccgaaggcgc agatcttgcg ggccttcagc ggctccgcct
     3661 catcgatcca tacgccggtt tgccccgtga tccggccggc tttgatgcca tacgtctcca
     3721 atgtgccgat gatcgcttct tcgagtgtgc gcaggtagcg gtgtatatcg gtgaagtgct
     3781 gg
//
LOCUS       DAOQOC010000137         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11430_length_3772_cov_3.455475, whole genome shotgun sequence.
ACCESSION   DAOQOC010000137 DAOQOC010000000
VERSION     DAOQOC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11430_length_3772_cov_3.455475"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="PL106_03270"
     CDS             <1..1681
                     /locus_tag="PL106_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32118.1"
                     /translation="LFFFTGLAIVIYLNQTPLQPRERDYAYVGSFYAFAIWIGLGVYA
                     LFDAARTLTWKELAYGAGGILGVGVLKYAVEGAVGDDHSVSFCLLYMGGVAGAALGLM
                     YLLRSIKNDAVPAVLATLLGLVIPAVMVADGWDDHDRSTRTPARDLAADYLESCPPQA
                     ILFTNGDNDTFPLWYAQEVEGIRTDVRVVNLSLLNTDWYIDQMRRKAYESEPVPFSLA
                     PEKYRQGTRDVVAIIPRDKGAGMDLKKAIDFAADDKNMQPIFSRTNKDAFLPTQTFRL
                     SADSAFVFGPQGMLTAKDTARFTPVMEWSLKKQYILKNHFMVLDLVANNNWKRPICFA
                     VTTGPDSYVNMQDYFRLEGLAYRLVPVYSKDQDPNTYGSVGTDIMYHNVMDKFKWGNM
                     DTEGFLYLDENVLRMTTNLRLQLATLANALAEEGHKDKAKEVLDLCMEKMPERNVPFD
                     RIMLPVIEAYYTAGDTTTANSLADRLFTIMDENMAWYLTLDPNFAARVSSEMEITHLV
                     MQRIAQSTSLLHGQKELGDKFTARLEEIEELYNAKLEDIATQGRRSSKVRF"
     gene            1692..2345
                     /locus_tag="PL106_03275"
     CDS             1692..2345
                     /locus_tag="PL106_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA32119.1"
                     /translation="MIRPPALEDAAGHLLRLDGRMLWRVPTAREARTVHLTFDDGPDP
                     EVTPWVLDTLGVLGVKATFFCVGRNIQAHPAVFKRLRDEGHAIGNHTWDHGDAWRTQA
                     RAWYREVLACQQLTGTRLFRPPYGHLTNRAIAALRRRFQVVMWDVLAHDYEEGWTDQQ
                     RIALTVGRVRPGSIIVFHDSPKCAARMRASMPEVVRRLKTAGYRFDILPETLATSRR"
     gene            complement(2329..>3772)
                     /locus_tag="PL106_03280"
     CDS             complement(2329..>3772)
                     /locus_tag="PL106_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA32120.1"
                     /translation="RLSIFAAHKAGDGWRGAEPFALNNAETSTGHPALSPDGHEFYFV
                     SDMPGGHGGTDIYVCKDRGGRWSEPKNLGPAVNTIGNEVFPFVGADGTLYFSSNGLPG
                     LGGLDIFAAKRGEDGDFTVAINVGAPVNGPKDDFGFIIDKAGKTGYFTSNRPGGAGDD
                     DIYAFEMHSALEQRYLCTGTVIDDETGLPLVEVEVTLYDDKGGTVESRQTDLNGKYSF
                     VVEKDREYRVTARMKGRFDGEQHLSTENIDKLQIVARDIHLVPDAGVWLRGAVRNKDR
                     IGFAEGVTVSVVNMNTFYTETRSTGPGGDFGFRLQTNEQFEVMLEKQGCYSQSVSVST
                     KGMTRGVIDLNEATDLRMEEIAIGRPIVLKHQKWAPGSAVLDPIAKTELDALADRLQV
                     NPRLNVEIGVHSDSRGNAEDELKLTQKRAEAVVTYLKGKGINKDRLAAKGYGMTRPVN
                     GCGPGVTCSEADHAENRRTEYTVTGIIDAR"
BASE COUNT          703 a         1304 c         1074 g          691 t
ORIGIN      
        1 cctgttcttc ttcaccggcc tggccatcgt gatctacctc aaccagaccc cgcttcagcc
       61 gcgcgaacgc gactatgcct acgtgggctc cttctacgcg ttcgccatct ggatcggcct
      121 cggcgtgtac gcgctcttcg atgcggcccg cacgctcacc tggaaggaac tggcctacgg
      181 cgcaggtggc atcctcggtg tcggcgtgct caaatacgcg gtggaaggtg ccgtgggcga
      241 tgaccactcc gtttcgttct gcctcctgta catgggtggc gttgccgggg ccgcgctcgg
      301 actcatgtac ctgttgcgaa gcatcaagaa cgatgctgtg cccgccgtgc tcgccaccct
      361 cctcggactg gtgatccccg ccgtgatggt ggccgatggc tgggacgacc acgaccgcag
      421 tacccgcacg cccgcacgcg acctggccgc cgactacctg gagagttgcc ccccgcaggc
      481 catcctcttc accaacggcg acaacgacac cttccccctc tggtacgccc aggaggtgga
      541 gggcatccgc accgatgtgc gcgtggtgaa cctcagcctg ctcaacaccg actggtacat
      601 cgaccagatg cgtcgcaagg cgtacgagag tgaacccgtg ccgttcagcc tcgccccgga
      661 aaaataccgg cagggcacac gcgatgtggt ggccatcatc ccgcgcgaca agggtgccgg
      721 catggatctg aagaaggcca tcgatttcgc ggcggacgac aagaacatgc aaccgatctt
      781 ctcgcgcacg aacaaggatg cgttcctgcc cacccagacc ttccgcctgt ccgccgacag
      841 tgccttcgtc ttcgggccac agggcatgct caccgcaaaa gataccgccc ggttcacacc
      901 ggtgatggag tggtcgctca agaagcagta catcctcaag aaccacttca tggtgctcga
      961 cctcgtggcc aacaacaact ggaagcggcc catctgcttc gccgtcacca ccgggcccga
     1021 cagctacgtg aacatgcagg actacttccg cctggaaggc ctcgcatacc ggctggtgcc
     1081 cgtgtacagc aaggaccagg accccaacac gtacggctcc gtgggcacgg atatcatgta
     1141 ccacaacgtg atggacaagt tcaagtgggg caacatggac accgagggct tcctgtacct
     1201 ggacgagaac gtactgcgca tgaccaccaa cctgcgcctg caactggcca cgctggccaa
     1261 cgccctggcc gaggagggcc acaaggacaa ggccaaggaa gtgctcgacc tgtgcatgga
     1321 aaaaatgccc gagcgcaacg tgcccttcga ccgcatcatg ctgcccgtga tcgaagcgta
     1381 ctacaccgca ggggacacga ccaccgccaa ctcgctggcc gaccggctgt tcaccatcat
     1441 ggacgagaac atggcctggt acctcacgct cgatccgaac ttcgcggcac gcgtatccag
     1501 cgaaatggag atcacgcacc tggtgatgca acgcatcgcg caatccacca gcctcttgca
     1561 cggccagaag gaactgggcg acaagttcac ggcgcggctc gaggagatcg aggagcttta
     1621 caacgccaaa ctcgaggaca tcgccaccca gggccgaagg agctccaaag tgcgcttctg
     1681 accttccgac catgatccgg ccaccagccc ttgaggatgc ggcaggccat cttctccggc
     1741 tcgatggccg catgctctgg cgcgtgccca cggcacggga ggcgaggacc gtccacctca
     1801 cgttcgacga tggtccggat cccgaagtga ccccatgggt tctggacacc ctgggcgtac
     1861 tcggcgtgaa agcgaccttc ttctgcgtcg gccggaacat ccaagcgcat ccggcggtct
     1921 tcaagcggct gcgggacgag ggccatgcca tcgggaatca tacctgggat cacggagatg
     1981 cctggcgcac ccaggcacgc gcctggtacc gcgaggtact ggcctgtcaa cagctgacgg
     2041 gaacgcgcct cttccgcccc ccgtacgggc acctcaccaa ccgcgccatc gccgccttgc
     2101 gccgtcgatt ccaggtggtg atgtgggacg tgctggccca cgactacgag gagggctgga
     2161 ccgaccagca gcggatcgcc ctcaccgtcg ggcgcgtgcg tccgggcagc atcatcgttt
     2221 tccacgacag cccgaaatgc gccgcacgca tgcgtgcgtc catgcccgag gtggtccgga
     2281 ggttgaagac cgcgggttac cggttcgata tcctgccgga aaccttggct acctcgcgtc
     2341 gatgataccg gtcaccgtgt attccgtgcg ccggttctcc gcgtggtccg cctcggaaca
     2401 ggtgacgccc ggaccacaac cgttcaccgg ccgcgtcatg ccgtagccct tggccgcaag
     2461 cctgtccttg ttgatgccct tccccttcag gtaggtcacg accgcttcag cgcgcttctg
     2521 tgtgagcttc agttcatcct ccgcgttgcc acgcgaatca ctgtgtaccc cgatctccac
     2581 gttcaatcgc gggttcacct gcaaccggtc ggccagcgca tccagttccg tcttcgcgat
     2641 cggatccaga acagcgctac ctggcgccca tttctgatgc ttcagcacga tggggcgtcc
     2701 gatggcgatc tcctccatgc gcaggtccgt ggcctcattc aggtcgatca ccccgcgcgt
     2761 catgcccttg gtgctcaccg acacgctctg cgaatagcaa ccctgcttct cgagcatcac
     2821 ctcgaactgc tcgttggtct gtagccggaa accgaaatcg cctccgggac cggtcgatcg
     2881 ggtctccgtg tagaaggtgt tcatgttgac cacgctcacc gtgacacctt cggcgaagcc
     2941 gatccggtcc ttgttgcgca cggcaccacg cagccagacc ccagcatcgg gcaccaggtg
     3001 gatgtcgcgt gccacgatct gcaacttgtc gatgttctcg gtgctgaggt gctgttcccc
     3061 atcgaaacgt cctttcatcc gcgcggtcac acggtactcc cggtcctttt ccacaacgaa
     3121 cgaatacttc ccgttcaggt cggtctgccg gctctccacc gtcccgccct tatcgtcgta
     3181 aagggtcact tccacctcca ccagcgggag gcccgtctcg tcgtcgatca ccgtccccgt
     3241 acacaggtaa cgttgctcga gtgccgaatg catctcgaac gcgtagatgt cgtcatcgcc
     3301 cgcccctccc ggccggttac tggtgaaata ccccgtcttc ccggctttat caatgatgaa
     3361 gccgaagtcg tccttcggac cgttcaccgg agcgcccacg ttgatcgcca cggtgaaatc
     3421 gccgtcctcg ccgcgcttcg ccgcgaagat gtcgaggccg cccagcccgg gcagaccgtt
     3481 gctggagaag tacagcgtgc catcagcacc cacgaagggg aacacctcat tgccaatggt
     3541 gttcaccgcg gggcccaggt tcttcggttc gctccatcgc ccgcccctgt ccttgcatac
     3601 atagatatcg gtacccccat gaccaccggg catgtcactg acgaaataga actcatgccc
     3661 atcgggcgaa agtgcgggat ggcccgtgga ggtctccgca ttgttcagcg cgaacggttc
     3721 ggccccgcgc cacccgtcgc ccgccttgtg cgcggcgaag atgctgagac gg
//
LOCUS       DAOQOC010000138         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11475_length_3764_cov_3.472634, whole genome shotgun sequence.
ACCESSION   DAOQOC010000138 DAOQOC010000000
VERSION     DAOQOC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11475_length_3764_cov_3.472634"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PL106_03285"
     CDS             complement(<1..382)
                     /locus_tag="PL106_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA32121.1"
                     /translation="MEVNENLSEKAVYDLDLVRRAVDKKDQKAYAELMSRYRDSIYFM
                     LLKMINNKDDAEDLTIEAFGKAFNRLHQYTPNFAFSTWLFKIASNNCIDWIRKQKKKR
                     MLSIDAPIGTDDGDEMHIELKGQGP"
     gene            complement(373..1491)
                     /locus_tag="PL106_03290"
     CDS             complement(373..1491)
                     /locus_tag="PL106_03290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA32122.1"
                     /translation="MSTLVPYILWALIAALIVLALYHFGAFIRLALKGLRTDVEPDRS
                     LPVSIIVCARDESVSLQQLIPLLMDQDHKEFEVVVVNDRSNDDTWEILQWMKPNFPRL
                     NPVNIQADAKFSYGKKLALGVGIRSAKHPHVLLTDADCMPVSRDWVSTMSAGFRNGKQ
                     LVIGFSPYEKTGGLTNLVERFDGISKGVQYLSFARAGFPYMGTGRNLGYTKDTFLAVQ
                     GGTRSHTALMSGDDDLFVNEVARARNTVAVSDGRSFMTTRGTPDMITWLRRKRRHYTT
                     ARHYRFIHQVLLTLLPMARAVMWAATVLLLVAGQWPWAVAALAIELCAFLPVRVGAMR
                     RLGAGRIVWFTIPLEWLFLILEPALYFSTILIPPRRWK"
     gene            1763..2899
                     /gene="tgt"
                     /locus_tag="PL106_03295"
     CDS             1763..2899
                     /gene="tgt"
                     /locus_tag="PL106_03295"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNA32123.1"
                     /translation="MKFVLHTTDPDSKARAGELHTDHGAVPTPVFMPVGTLGAVKTVH
                     MRELREDVKAPMMLSNTYHLYLRPGTNVLEHAGGLHKFNGWDRPILTDSGGFQVHSLS
                     DIRKITEEGVKFQSHIDGSRHLFTPENVMDIQRSIGADIIMAFDECTPWPCDVKYATD
                     SMHRTHRWLDRCIARFSGTEPRYGHAQALFPIVQGSTFPELRARSCEYIATKGAEGNA
                     IGGLSVGEPEEEMYAMAELCCDILPKDRPRYLMGVGTPWNLLENIALGIDMFDCVMPT
                     RNGRNGMLFTRRGVVQIKNKCWSDDHSPLDPDGTCWVDQQYSRALVRHLFTTNEMLGQ
                     QIASLHNLTFYIDLMREAREHILDGSFSTWKARTLPLLKQRITG"
     gene            3014..>3764
                     /locus_tag="PL106_03300"
     CDS             3014..>3764
                     /locus_tag="PL106_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNA32124.1"
                     /translation="MLKTLDRYIIRQFLGTFFLLLVLIMAIAVVFDISEKTGDFAKSH
                     ATTREIVVDYYVNFILYYANFFSGLFIFLAVLLFTSRLAGRSEVIAMLSGGVSFNRFV
                     RPYMIAATFLAAISLYLNVFLLPGANRTRLAFEKAHIWNPYRVEGNDLLREVAPGVIV
                     YMHGMDMPTLTATHMSVARWENGELRSKLEADHAVYDTVSGKWRFVDWRIRTIPVPVG
                     GVLTNPHEVLSKGHELDTLLPLRPREPRCTWD"
BASE COUNT          730 a         1162 c         1183 g          689 t
ORIGIN      
        1 cgggcccttg ccccttcagc tcgatgtgca tctcgtcgcc atcatcggtg ccgatgggcg
       61 catcgatgct gagcatgcgc ttcttcttct gcttgcggat ccagtcgatg cagttgttcg
      121 acgcgatctt gaagagccag gtgctgaagg cgaaattggg cgtgtactga tggagacggt
      181 tgaaggcctt gccgaacgcc tcgatggtga gatcctcagc atcgtccttg ttattgatca
      241 tcttcagcag catgaagtag atcgaatcgc ggtagcgcga catcagttcg gcgtaggcct
      301 tctggtcttt cttgtccacc gcgcggcgca cgaggtccag gtcgtacacc gccttttcac
      361 tgaggttctc gttcacttcc atcggcgtgg gggtatgagg atggtgctga agtacagcgc
      421 cggctccagg atgaggaaca accattcgag ggggatcgtg aaccatacga tgcggcccgc
      481 accgaggcgg cgcatggcgc ccacgcggac cggcaggaaa gcacaaagtt cgatcgcgag
      541 cgccgccacg gcccaaggcc attggccggc cacgagcagc agcacggtgg cggcccacat
      601 caccgcgcga gccatgggca gcagggtgag cagtacctgg tggatgaagc ggtagtggcg
      661 cgccgtggtg tagtggcggc gtttgcggcg gagccaagtg atcatatccg gcgttccgcg
      721 cgtggtcatg aacgagcgcc catcggacac ggcgacggta ttcctcgcac gggccacttc
      781 attcacgaac aggtcatcgt cgccgctcat gagcgccgtg tgcgatcgag tgccgccctg
      841 aacagccagg aaggtgtcct tggtgtagcc caagttgcga ccggtgccca tgtacgggaa
      901 cccggcccgc gcgaagctga ggtactgcac ccccttgctg atgccatcga accgttcgac
      961 caggttggtg aggccgccgg tcttctcata cgggctgaag ccgatcacca gttgtttccc
     1021 gttgcggaaa ccggcgctca tggtgctcac ccaatcccgg ctcacaggca tgcaatcggc
     1081 atcggtcagc agcacatgcg gatgcttcgc gctgcgaatg cccacgccca aggccagctt
     1141 cttcccgtaa ctgaacttgg catcggcctg gatgttcacc gggttcaggc gggggaagtt
     1201 cggcttcatc cattggagga tctcccaggt gtcatcgttg ctgcggtcgt tcaccacgac
     1261 cacctcgaac tccttgtggt cctggtccat gaggagcggg atcaattgct gcaacgacac
     1321 gctttcgtcg cgcgcgcaca cgatgatgct cacgggcagg ctccggtcgg gttcgacatc
     1381 ggtcctcagt cctttcagtg cgagccggat gaacgcaccg aagtgataga gcgccagcac
     1441 gatgagtgcg gcgatcaggg cccagaggat gtacggtacg agggtggaca acacggaagg
     1501 ccgcagggaa cggtatcggg gcgaagctac cgggaggctc ccgggggaca gggtgcgggc
     1561 cgggcagtta tgaacagcgg gggcgtggaa gggagccttc gctcgccctg tcggcaggaa
     1621 tgcggatctc ccggtccgga ccggcaccat gcgtttcccc ctaaggaatg gccggcaaat
     1681 acggtccgac cagtgcggtg tccgacatca gcatgggctt ggccttcggg accacgcctg
     1741 aagccccatc ttcgcggccc gcatgaaatt cgtcctccac accaccgacc ccgacagcaa
     1801 ggcccgagcg ggtgaattgc atacggacca cggcgcggta cccacacccg tgttcatgcc
     1861 cgtggggacc ctgggtgcgg tgaaaaccgt tcacatgcgc gagttgcgcg aggacgtgaa
     1921 ggcccccatg atgctcagca acacgtacca cttgtacctt cggccaggta cgaatgtgct
     1981 ggagcacgcg ggaggcctgc acaaattcaa cggctgggac cgtcccatcc tcaccgacag
     2041 cgggggcttc caggtgcatt cgctcagcga catccgcaag atcaccgagg agggcgtcaa
     2101 gttccagagc cacatcgacg gcagccgcca cctcttcacc cctgagaacg tgatggacat
     2161 ccagcgcagc atcggggccg acatcatcat ggccttcgac gaatgcacgc cgtggccgtg
     2221 cgacgtgaag tacgccaccg acagcatgca ccgcacgcac cgctggctgg accgatgcat
     2281 cgcgcgtttc agcggcacgg agccgcgcta cggacatgcg caagcgcttt ttcccattgt
     2341 gcagggcagt acattcccgg aattgcgcgc gcgcagctgc gagtacatcg ccacgaaagg
     2401 cgcggaaggc aatgccatcg ggggcctcag cgtgggcgag cccgaggagg agatgtacgc
     2461 catggccgag ctgtgctgcg acatcctgcc caaggaccgg ccacgctacc tgatgggcgt
     2521 gggaacaccg tggaacctgc tggagaacat cgccctgggg atcgacatgt tcgactgcgt
     2581 gatgcccacg cgcaacggcc gcaacggcat gctcttcacc cggcgtggcg tggtacagat
     2641 caagaacaaa tgctggtccg atgaccactc gccactggat ccggacggca cctgctgggt
     2701 ggaccagcag tacagcaggg ccctcgtgcg ccacctgttc accaccaacg agatgctcgg
     2761 ccagcagatc gccagcctgc acaacctcac cttctacatc gacctgatgc gcgaggcccg
     2821 cgaacacatc ctggatggca gtttcagtac atggaaggca cggaccttgc cactgctgaa
     2881 gcaacggatc acgggttgat cggaagcggg gccggacctt gcttcgttcc gatcttccac
     2941 gatcttcacc gcgcgaacga tccgccttcg gccggttcat gaacaggatc atcatcagga
     3001 agaacagatc gtgatgctga agacgctgga ccggtacatc atcaggcagt tcctcggcac
     3061 cttcttcctc ctgctggtgc tcatcatggc catcgccgtg gtgttcgaca tcagcgagaa
     3121 gacgggcgac ttcgccaaga gccacgccac cacccgggag atcgtggtgg actattacgt
     3181 gaatttcatt ctttattacg ccaatttttt cagcggcctg ttcatcttcc tggccgttct
     3241 gctgttcacc agtcggctgg cggggcgcag cgaggtgatc gccatgctca gcggaggcgt
     3301 cagcttcaac cgcttcgtgc ggccgtacat gatcgcggcg accttcctgg ccgccatatc
     3361 actgtacctg aacgtgttcc tgctgcccgg cgccaaccgc acgcgcctgg ccttcgagaa
     3421 ggcgcacatc tggaacccgt accgggtgga gggcaacgac ctgctgcgcg aggtggcgcc
     3481 gggggtcatc gtgtacatgc atgggatgga catgcccaca ctgaccgcca cacatatgag
     3541 cgtggcgcgg tgggagaacg gcgaactgcg ctcaaagctg gaggccgatc acgccgttta
     3601 tgataccgta tcaggcaaat ggcggttcgt cgactggcgc atccggacca tcccggtgcc
     3661 ggtcggtggg gtgctgacga acccgcacga ggtcctgtcg aagggccatg aattggacac
     3721 gctgctgccg ttgcggccaa gagaaccgag gtgtacatgg gatg
//
LOCUS       DAOQOC010000139         3758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11497_length_3758_cov_3.876046, whole genome shotgun sequence.
ACCESSION   DAOQOC010000139 DAOQOC010000000
VERSION     DAOQOC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11497_length_3758_cov_3.876046"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..915
                     /locus_tag="PL106_03305"
     CDS             256..915
                     /locus_tag="PL106_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32125.1"
                     /translation="MRTLTFLSVLAISSGIQAQKAIGAFGSAGEDLVWRTSQVNAGML
                     FEFRTRLIPPAVVRASMWWSPKRGNYHEEYRTTPQPDVYRWLAATRTERHHSAGIALD
                     LRFPFESNACSDGYFKGTYLLAGIGVVQRWLRTDRWEQDRNGNITTSVDRVFYVEPML
                     RAGAGGELNFKWGGPFIEGTVTVSAAGPDQPTFRFPGSALITLGYRYSFAKPEPVSGA
                     E"
     gene            complement(1048..1416)
                     /locus_tag="PL106_03310"
     CDS             complement(1048..1416)
                     /locus_tag="PL106_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32126.1"
                     /translation="MGGKQTTDDTYILKGTFTPNGGQQQQVNVDNFETSDKKGSTAIK
                     HFRKADVAVMAGLGYTLDMGFDVDLRYYRSLSTTYDEQSGSSRLRLWTNLVEHIPFRN
                     KDPSDLLALFPHGFSDVVVP"
     gene            complement(1576..>3758)
                     /locus_tag="PL106_03315"
     CDS             complement(1576..>3758)
                     /locus_tag="PL106_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32127.1"
                     /translation="NKTTVMRWDGSSWSAVGSSGISAGVAWSPSLALDPSGNPVVAYR
                     DGANGQRTTVMRWDGTSWITMGTAGFTGVNTSNSTRWLQVDAHGRLVVAYASGYMYAK
                     RYGHTITTDAIAGSPFCAGASVSVPFTTFGTFSLGNTFTAELSDASGSFASPVVIGSL
                     VSNVSGTIACIIPPGTPGGTGYRIRVVSSNPAVTGTDNGSGLTINAPSLWYADSDGDG
                     YGDPAVSVLACAQPVAHVSNFGDCNDTDVNLTVIGLPCDDGSASTENDVVTSLCTCEG
                     TPLGGPCTENEVTLDLSTDAASTETTWDIVLDGTNVVVCNGGGYGPNGSFTATCCLPD
                     GCYDLRVFDSGGDGIDPGGFTLRDANGDRILDNAGNGAYFGSQSEAPFSFCVPIGTTS
                     FDPSSCDVLNATPNTVLHVTPIPAVTSVYSPGTPWANANTGYHYWIFNPHGGYNRRIT
                     LTHAVPGSGYPPSTPVDLRCSYLKLTQIQSFPVPLNVPLNIRVRTLINGVYGEFGPAC
                     KLFLPTPACPVSQLTTAADPVVSCGATGLSLGGTIWADQVTSATNYQFEFSRPSYLRR
                     ITSSTRSQALNFYTYPLMDNTCYNVRVRVSFDGGTTFCPFGPYCTITLGTAICGFGEM
                     PPMVEDDNFTDLVVMDEQLTLWPNPNDGSRINLALTGSARSAGTVQVELTDGCGKLVI
                     TEVLPAHDGQFRTSLDLDRALAPGLYLVKVRAGDQMHTRRLVIQ"
BASE COUNT          740 a         1121 c         1188 g          709 t
ORIGIN      
        1 gtcgggcagt tcctgggcgg aggcggtggc ggcgaacagg gccagggcga ggctggtggc
       61 ggcgtatcgg acgttcatgc gcggaagggg ttttgggtta cagagcgaac cccgtgccaa
      121 ggggcttggg cggcgaactt acccaaacac gttgtgccga gcgtcctcct gaccggaaca
      181 gggtctatct tgggctccgg gcatcgccga agccgatacc aaagtgcctg cccgtacgtt
      241 gcaccaagca tgccgatgcg cacactcacc ttcctttcgg tgctcgcgat cagttcgggc
      301 atccaggcgc aaaaggccat cggagcgttc ggcagtgcgg gtgaagacct ggtctggcga
      361 acgagccagg tcaacgccgg gatgcttttt gagttccgta cgcggttgat acctcctgcc
      421 gtggtgaggg cctccatgtg gtggtccccc aagcgcggca actaccacga agagtaccgg
      481 acgaccccgc agcccgatgt ataccgctgg ctggcggcga ccaggacgga gcggcaccac
      541 tcggcgggca tcgcactgga cctgcgcttc ccctttgagt ccaacgcttg ctcggacggc
      601 tacttcaagg gcacctacct gttggcgggc atcggcgtcg tacagcgctg gctgcggacg
      661 gaccgatggg aacaggaccg caacgggaac atcaccactt ccgtggaccg tgtgttctac
      721 gttgaaccca tgctccgtgc gggcgcgggt ggagaactga acttcaaatg gggcggccct
      781 ttcatcgagg gcacggttac ggtatcggcc gcaggaccgg atcaacccac attccgtttc
      841 cccggtagcg cactgatcac cctgggatat cgttacagct tcgcgaagcc cgagccggta
      901 agcggggcgg agtgatcacg cctccacagg cctactcgcg attgaacgtc caacccagtg
      961 taatacccta tcggaataaa gggccgtcca aagatgcgcg gcaatttttt cgcagggcga
     1021 ttcgaggtgg gcgttgcgta gcggtgtcta cggaacgacc acatcggaga agccatgcgg
     1081 aaaaagagca agcagatcgg atggatcctt attccgaaag ggtatatgct ccaccaggtt
     1141 ggtccacaag cgaaggcgtg agctcccgct ctgctcatcg tacgtggtgc tcagactgcg
     1201 gtagtagcgc aggtccacat cgaagcccat atccaaggtg taacccagac cggccatcac
     1261 ggccacatcg gccttgcgga aatgcttgat ggccgtactg cctttcttgt ccgaggtctc
     1321 gaaattgtcg acgttcacct gctgctgctg gccgccgttg ggtgtgaagg tgcctttcag
     1381 gatgtacgtg tcatcggtgg tctgcttgcc gcccatgaga aaatggtaga gtgggcccgg
     1441 atcaggagcg gcacaactgg tactgtgacg gaggtctatc cgaaccgctc gttcataccc
     1501 gagcagctca tggcagcatg aaacacagag ccccgtccac tggagtgaac ggggctccaa
     1561 gctcgtgttc agcccctatt ggatcaccaa gcgacgggta tgcatttggt ctccagcacg
     1621 caccttcacc agatacaggc cgggagcgag ggccctatcc aggtccaggc tggtacggaa
     1681 ctgcccgtcg tgggcaggca ggacctcggt gatcaccaac ttaccgcagc catcggtcaa
     1741 ttccacctgt accgttcctg cgctgcgcgc agatccagtg agcgccaagt tgatccgtga
     1801 tccgtcgttg gggtttggcc ataaggtcaa ctgttcatcc atgaccacca gatccgtgaa
     1861 gttgtcgtcc tcgaccatcg gaggcatctc cccgaagccg cagatcgccg taccgagtgt
     1921 gatggtgcag tacggtccga aaggacagaa ggtggtgcca ccgtcgaagc tcacccgcac
     1981 ccgcacgttg taacaggtgt tgtccatcag cggataggtg tagaagttca acgcctgcga
     2041 acgagtggat gacgtgatcc ggcgcaagta gctcgggcgg ctgaactcga actggtagtt
     2101 ggtggcgctg gtcacctggt ccgcccagat ggtaccgcca aggctgaggc ccgtggcacc
     2161 gcaggagacc acaggatccg cggcggtggt gagctggctg acgggacaag cgggcgtggg
     2221 caggaacaat ttgcaggccg ggccgaactc gccgtacacc ccattgatca aggtgcgcac
     2281 acggatattc aacggtacgt tcagtgggac cgggaaggac tgtatctggg tcagcttgag
     2341 gtaggaacaa cgcagatcca ccggggtgct cggtgggtag ccactacccg gaacggcgtg
     2401 cgtgagggtg atgcggcgat tgtatccacc atgtgggttg aagatccaat agtggtaccc
     2461 tgtattggcg ttggcccacg gcgtgccagg actgtacacc gaggtgaccg ctgggatcgg
     2521 cgtcacatgc aacaccgtgt tgggcgttgc attcaacaca tcacaactcg acggatcgaa
     2581 gctggtggtg ccgatgggca cgcagaagga gaagggggcc tcgctctgag agccgaagta
     2641 cgctccattc ccggcgttgt ccagtatgcg atcaccgttg gcatcgcgca gcgtgaaccc
     2701 gcccgggtcg atcccatcac ctccgctgtc gaacacgcgc agatcgtaac aaccgtcggg
     2761 caggcagcag gtcgcggtga aactgccatt ggggccgtat ccgccaccgt tgcataccac
     2821 aacattggta ccatccaaca cgatgtccca ggtggtctcg gtcgatgcgg catccgtgct
     2881 gaggtccagg gtcacttcgt tctcggtgca aggaccacca aggggcgtgc cttcgcaggt
     2941 gcagaggctt gtgaccacat cgttctccgt gctcgcggag ccatcgtcgc aaggcagacc
     3001 gatcacggtg aggttgacgt cggtatcgtt gcagtcgccg aagttgctca cgtgggccac
     3061 gggttgagcg caggcaagca cgctcacagc cggatcgccg tagccgtcgc cgtcactgtc
     3121 cgcataccac aagctgggtg cattgatcgt gaggccgctc ccattgtcag tgcccgttac
     3181 cgccgggttg ctgctcacca cccggatgcg gtagcccgta ccaccgggcg tacctggtgg
     3241 tatgatgcag gcgatggtgc cgctcacgtt gctcaccagg ctgccgatga ccaccgggct
     3301 ggcgaagctg ccgctggcat cgctcaactc tgcagtgaag gtgttgccaa ggctgaaggt
     3361 accaaaagtc gtgaatggaa cgctcaccga ggcgccagcg caaaagggcg aaccggcaat
     3421 ggcgtccgtg gtaatggtgt ggccgtagcg cttagcgtac atgtaaccgc tggcgtaggc
     3481 caccaccaag cgcccgtgcg catccacctg caaccaacgc gtactatttg aggtgttcac
     3541 accggtgaat ccggccgtac ccatggtgat ccagctggtg ccgtcccagc gcatcactgt
     3601 ggtccgctga ccattggcgc catcgcgata ggccaccacc gggttgccgc tggggtcaag
     3661 cgccaagctt ggggaccagg ccactcccgc tgaaatgccg gagctgccca cggcactcca
     3721 actgctgccg tcccagcgca tcaccgtggt cttgtttc
//
LOCUS       DAOQOC010000140         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11544_length_3749_cov_3.933406, whole genome shotgun sequence.
ACCESSION   DAOQOC010000140 DAOQOC010000000
VERSION     DAOQOC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11544_length_3749_cov_3.933406"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2067)
                     /locus_tag="PL106_03320"
     CDS             complement(88..2067)
                     /locus_tag="PL106_03320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNA32128.1"
                     /translation="MNNSITTLSAAAGLLLLAACGQSENTKTPAKITEAPPPVIDMRD
                     FFKNPEKAGFQISPDGNYMSWRAPWKNRMNIFVKKMGDSAEVQVTQDTVRDIGGYFWK
                     GDRLVYSRDINGDENFILFSASIDGKDVKRLTPEKGVRAGTLDRLHNVPGMEQTVLIQ
                     MNQRNPQVFDPYTCNIATGELKPLYDNSKDNFEGWITDHAGVIRMATKTEGTDNVVYY
                     RATEKEPFAEYMRTGFKDSWGPLLFTFDNANLIVSHNLNGRDKTAIVEWDLAGKKETK
                     LIFENADYDVSNVDYSEKRKVLTMVTWTGEKSERHFLDDQTKAMYDKLAGKFTGLEYW
                     VYGQNDEETKFMVWAGSDRDPGTYYFYDAPADKLEKVATLRPWIDPTQMAEMKPISYQ
                     SRDGLTIHGYLTLPVGREAKNLPVVINPHGGPWARDDWGFNPEVQLLANRGYAVLQMN
                     FRGSTGYGRAFWEKSFKQWGKTMQDDITDGVEWLKKEGVADPARIAIYGGSYGGYATL
                     AGITYTPDLYACAVDYVGVSNMFTFMNTVPPYWEPFKKMMYEMVGDPQKDSLLLREAS
                     PVFFVDRIKCPLFIAQGAMDPRVNKDESDQVVAALKARGVSVQYLVKENEGHGFHNEE
                     NRFEFYGAMEKFLDQYIGSGFKPATAAADSVKKAA"
     gene            complement(2158..3522)
                     /locus_tag="PL106_03325"
     CDS             complement(2158..3522)
                     /locus_tag="PL106_03325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA32129.1"
                     /translation="MSTDRTAILLLLLLGAALKFSWLGASPLNGDEPFTLFWATRPWG
                     EFTAMLRTENNPPLYFLLVRAWVALVPWSEAWLRLPSAVFSVLTVWPLYLAARSMGGS
                     AMAVCAGLLFTLNNHQYIYAHELRGYSLLLLLAVVAIRLLLREARGPGWWSAGLLGLV
                     FAGLVWTHFFGWLVIGLCLLCSLVLPELRAARRRTFLATCIAAVAFLPYAYIFFSRAG
                     ESIAQGTWVGPQGIDEIWHMIRRWSNQPVVTILLLMPVVVVLVRERLSALALRFGLLW
                     WLVPLVGLWVVQWWVPAYLDRYLLFASPGFYLATAYGLVHCDPGGHVRWLAPVVGVLG
                     MVITFTPWKTNGNQPDRVAAQVRTWQEENAGRPVVVRPFWYKPTLWAHMERSPAPAPW
                     LDTWSERYDDARLPGPLEDRGSFILVYASPATGDEDAPAFPGFHVQADRRCDRLMHVA
                     LYTN"
BASE COUNT          669 a         1199 c         1163 g          718 t
ORIGIN      
        1 aacaggtcgt gtcgatcctt ggcggcccga tgaaagcgga acggcggagc atgaaagctc
       61 cgccgttccg cccacagatc gcgccggtca ggccgccttc ttcacgctgt cggcggctgc
      121 cgtggccggc ttgaacccgc tgccgatgta ctggtccagg aatttctcca tagccccgta
      181 gaactcgaag cggttctcct cgttgtggaa accgtggcct tcgttctcct tcaccaggta
      241 ctgtacgctc acgccacggg ccttcagcgc ggccaccacc tgatcgctct catccttgtt
      301 gacccgcggg tccatggccc cctgcgcgat gaacagcggg catttgatgc ggtccacgaa
      361 gaacacaggc gaggcttcgc gcaacaggag gctgtccttt tgcggatcac ccaccatctc
      421 gtacatcatt ttcttgaacg gctcccagta gggaggcacc gtgttcatga aggtgaacat
      481 gttgctcacc cccacataat ccaccgcaca ggcgtacagg tcgggggtgt aggtgatgcc
      541 ggccagcgtg gcgtacccgc cgtagctgcc cccgtagatc gcgatgcggg ccggatcggc
      601 gaccccttct ttcttcaacc attccacgcc gtccgtgatg tcgtcctgca tggtcttgcc
      661 ccactgcttg aagctctttt cccaaaaagc gcggccgtag ccggtgctcc cccggaagtt
      721 catctgcagc acggcgtagc cccggttggc cagcagttgc acctcggggt tgaagcccca
      781 gtcgtcgcgg gcccaagggc ctccgtgcgg attgatcacc accggcaggt tcttcgcttc
      841 gcggcccacg ggcagggtga ggtagccatg gatcgtgagc ccatcgcggc tctggtagct
      901 gatgggcttc atctccgcca tttgagtcgg gtcgatccac ggccgcagcg tggccacctt
      961 ctcgagcttg tctgccgggg cgtcatagaa gtagtaggtg cccgggtcgc ggtcgctgcc
     1021 ggcccatacc atgaatttgg tctcctcgtc gttctggccg tacacccagt attcaagccc
     1081 tgtgaacttg cccgcgagct tgtcgtacat ggccttggtc tgatcgtcca ggaagtggcg
     1141 ttcggacttc tcgcccgtcc aggtgaccat ggtgaggacc ttgcgcttct cgctgtagtc
     1201 cacgttgctc acgtcgtaat cggcgttctc gaagatcagc ttcgtctcct tcttgccggc
     1261 caggtcccac tcaacgatcg cggtcttgtc gcggccgttc aggttgtggc ttacgatcag
     1321 gttcgcgttg tcgaaggtga acagcagtgg accccagctg tccttgaagc ccgtgcgcat
     1381 gtactcggca aagggttcct tttccgtggc gcggtagtac accacgttgt cggtgccttc
     1441 ggtcttggtg gccatgcgga tcacacccgc gtggtcggtg atccagccct cgaagttgtc
     1501 tttgctattg tcgtagagcg gtttcagctc ccccgtggcg atgttgcacg tgtaggggtc
     1561 gaacacctgc gggttgcgct ggttcatctg gatcaggacc gtctgttcca tgccaggtac
     1621 gttgtgcagg cgatccaggg tgcccgcccg cacgcccttc tccggcgtca ggcgcttcac
     1681 gtccttgccg tcgatgctgg cgctgaaaag gatgaagttc tcatcgccat tgatgtcgcg
     1741 gctgtacacc aggcgatcgc ccttccagaa gtagccgccg atgtcgcgca cggtgtcctg
     1801 ggtcacctgc acttcggcac tgtcgcccat cttcttcacg aagatgttca tgcggttctt
     1861 ccacggcgcg cgccaactca tgtagttgcc gtccgggctg atctggaaac cggccttctc
     1921 cgggttcttg aagaagtcgc gcatgtcgat caccggaggt ggggcttcgg tgatcttggc
     1981 cggtgttttc gtgttctcgg actggccgca cgcggccaga agcaacaagc ctgccgcagc
     2041 ggacaaggtg gttatggagt tgttcatggg tatcggggct ggcaaagatg gtcgtacaac
     2101 gcaccgatcc atcggagcgg gatgagcggt tcatccccgg gatggatcgt tcagcggtta
     2161 gttggtgtac agcgccacgt gcatcaaccg gtcgcagcgg cggtccgcct gcacatggaa
     2221 ccccggaaat gcgggggcgt cctcgtcgcc tgtggccgga gaggcataga cgaggatgaa
     2281 actcccgcga tcctccagcg ggccgggaag acgcgcatca tcgtaacgtt ccgaccaggt
     2341 gtccagccag ggcgctggcg cgggggagcg ctccatgtgg gcccacaggg tgggcttgta
     2401 ccagaagggc cggaccacca cgggccgccc ggcgttctcc tcctgccagg tccgaacctg
     2461 ggccgccacc cgatcgggct ggttgccgtt ggttttccac ggggtgaagg tgatgaccat
     2521 ccccaacacc ccgaccaccg gcgccaacca tcggacgtgt cccccgggat cgcaatgcac
     2581 aagcccgtat gccgtagcca ggtagaagcc gggactcgcg aagagcagat agcggtccag
     2641 gtacgcgggc acccaccact gcaccaccca aaggcccacc agtgggacca gccaccagag
     2701 caggccgaac cggagtgcga gggcggacag gcgttcgcgt acaagcacca ccaccacggg
     2761 catcagcaac aggatcgtga ccaccggttg gttgctccag cgccggatca tgtgccagat
     2821 ctcgtcgatc ccctgcggcc ccacccatgt gccttgcgcg atgctctccc cggcgcggct
     2881 gaaaaaaata tacgcgtacg gaaggaatgc gacggcggcg atgcaggtgg ccaggaacgt
     2941 gcgacgacgc gcggcccgca gttcgggcaa taccagcgaa cagagcaggc acaggccgat
     3001 gaccagccaa ccgaagaagt gcgtccagac cagcccggcg aacacgaggc ccagcagccc
     3061 ggccgaccac caccccgggc cacgcgcttc gcgcaggaga agccggatgg ccaccaccgc
     3121 cagcagcagg agcagcgaat aaccccgcag ctcgtgcgca taaatgtact ggtggttgtt
     3181 gagcgtgaag agcagacccg cgcacacggc cattgcgctg ccgcccatgg agcgcgctgc
     3241 gaggtacagt ggccatacgg tgagcacgct gaacacggcc gatggcaacc gcagccaggc
     3301 ctcgctccac ggtaccaatg ccacccacgc acgcaccagc aggaagtaca gcggcgggtt
     3361 gttctccgtg cgcagcattg cggtgaattc gccccaaggc cgtgtggccc agaagagggt
     3421 gaaaggctca tcgccgttca acgggctcgc gcccagccaa ctgaacttga gcgccgcgcc
     3481 cagcagcagc aataggagga tggcggtgcg gtcggtggac atgaacggcc gaaactatcc
     3541 gcctcgaggt ccgaaccctt cggacgttcg tggtttttcc tgaactgtga cgccaccttt
     3601 ccatgagctg tgatacctct gggtcccgag caacatgaac gcacagtatg gagtggagac
     3661 catgagggag tatcatgtgt gtattgcgct ccttgcttcg ccgtcttcgc cgccgcgaag
     3721 cgctatggcg gagcgcggag cggctacgc
//
LOCUS       DAOQOC010000141         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11623_length_3735_cov_3.154348, whole genome shotgun sequence.
ACCESSION   DAOQOC010000141 DAOQOC010000000
VERSION     DAOQOC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11623_length_3735_cov_3.154348"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3541
                     /locus_tag="PL106_03330"
     CDS             <1..3541
                     /locus_tag="PL106_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNA32130.1"
                     /translation="GDHREQVIPVGRLAARNDQQVLDYLAKVQQLEAQQSVPAAWMKN
                     ILHFRGGFTTEELVLFQSTLDQYRTIAEDTCFTGKVTQFVKNPDDLISQAAADSVSDL
                     IQQGVTLMTFFAHASGGGFDISIDQPNNYQWNGRYPFMIGNSCYTGNIHLPSCGSGSE
                     QFVMPAGAGAIAFIASIDLALAQYLSLITREFYASFAHANYGGTIGRHIQYADSVFLH
                     NVPPDDDITHLTYWTTAQQFTLQGDPVLRMNSPLVPELAITANDVRLLPDPVTADVDS
                     FVVEATVRNLANACRCPRFPVAVKRQFGQGQQYTLVDSLVLDSFERTLPFTLPVLADS
                     GGAGVNNLTVEVDLQEPPVGLVDEYEDVANNSVTRQFIVVSGDIIPADPYNFAITPDP
                     APMLRASTGDPFAPVRNYVFQIDTTDTYDSPMMEHTTVSAPGGVVSWQPQGIYSLNQG
                     QDSLVFYWRCAVDSVGNNGYEWKEFSFQHITGRTGWGQAHHFQFKDTLANNSFSNMVF
                     DRPGRDFDFFTGLRSVGAVTQGNSSAICKWTRDLYTQEGSVGCAGGTPSIMMAVIDPF
                     DFTAWTSLFNGTGHHLGAYNSAVCNNVGHPFKAFFYRQNNPGEMAALAATLTSPDSIP
                     AGHYLLFYTLRYIDLPAITGSGLMTAFAAMGADTLNSGLVPDHAPYIFFTRKGDPAFG
                     QEVWSDDPNAIINIAVFLDANGNSGTMATPRSAEMLDWNSLHWRIVPEQEPSDSARIV
                     IRAVDNTQQYEGPFYTVDPAATTDSLYFTDIGLNGSQYPRVRLVGAYVSEQDGDARPA
                     QTKRWQIIGSPAPECAIDPPLGFFSHIDSLFVGQQAEVMVAVHNISDVAMDSLLMAAW
                     LTDGNNTRHTVHWRMRAPLPVGGVLLDTIRFELQAGWGGMNALVIEANPIDTATGLYD
                     QREQYHFNNIAILRFETFADRENPVLDVTFDGIHILDGDIVSAQPEVVMTLNDENTTL
                     LFDEVADTAYIKVYLLPPGSSNETLIPFTDPGGAEVMRFTPTNGPENECRIQWMPDRL
                     SDGVYRLRVRASDISGNQSGSHDLTVRFEVVNKPTITEVLNYPNPFTTSTRFVFTLTG
                     SETPTGMRIRIMTISGRVVREIMLDELGPLHIGRNITDYAWDGTDQFGDKLARGVYLY
                     QVAAQLHGEDIEHRASGADGFFKKGFGKMYLLR"
BASE COUNT          733 a         1214 c         1126 g          662 t
ORIGIN      
        1 tggcgaccac cgcgagcagg tgatcccggt cggacgcctc gcggcccgca acgatcagca
       61 ggtgctcgac tatctggcca aggtgcagca attggaggcg cagcagtccg tacccgcggc
      121 ctggatgaag aacatcctgc acttccgggg aggcttcacc accgaggagc tggtgctttt
      181 ccagagtacc cttgaccagt accggaccat cgccgaggac acctgtttca ccggcaaggt
      241 gacccagttc gtgaagaacc ccgatgacct gatctcccag gccgccgccg acagtgtatc
      301 cgacctgatc cagcaagggg tcaccctgat gaccttcttc gcccatgcca gcggtggtgg
      361 attcgacatc agcatcgacc agccgaacaa ctaccagtgg aacgggcgct atccgttcat
      421 gatcgggaac tcgtgctaca cgggcaacat ccacctgccc tcgtgcggca gcgggagcga
      481 acagttcgtc atgccggccg gtgcgggcgc catcgccttc atcgccagca tcgatctggc
      541 cctcgcgcaa tacctgagcc tcatcacccg cgagttctac gcgagcttcg cgcatgccaa
      601 ttacgggggc accatcggca ggcacatcca gtacgccgat tcggtcttcc tgcataatgt
      661 accgccggac gatgacatca cccatctcac gtactggacc acggcgcagc agttcaccct
      721 gcaaggggat ccggtccttc ggatgaactc gccactggtg ccggaactgg cgatcaccgc
      781 gaacgacgta cggctcctgc ccgaccccgt gacggccgac gtcgacagct tcgtggtgga
      841 agcaacggta cgcaacctgg ccaacgcgtg ccgttgtccg aggttccccg tggccgtcaa
      901 gcggcagttc ggtcaggggc agcagtacac cctggtcgat tcactggtgc tcgacagctt
      961 cgagcgcacg cttccattca cgctgcctgt cctggccgac agcggtgggg ccggcgtgaa
     1021 caacctgacc gttgaggtgg acctgcagga accgcctgtc ggcctcgtgg acgagtacga
     1081 ggatgtcgcc aacaactcgg tcacacggca gttcatcgtg gtgagcggag atatcatccc
     1141 tgcggacccg tacaacttcg ccatcacgcc cgacccggct cccatgttgc gggccagcac
     1201 cggcgatccc tttgcgccgg tacgcaatta cgtgttccag atcgacacca ccgacacgta
     1261 cgacagcccc atgatggagc acaccacggt gagcgcgccg ggtggcgtgg tcagctggca
     1321 accccagggt atttattcgt tgaaccaggg ccaggacagc ctggtcttct actggcgatg
     1381 cgcggtggac agtgtcggca acaacggtta cgaatggaag gaattcagct tccagcacat
     1441 caccgggcgc accggatggg ggcaggcgca ccacttccag ttcaaggaca cgctggccaa
     1501 caacagcttc agcaacatgg tcttcgacag gccaggccgc gacttcgatt tcttcaccgg
     1561 cctgcgcagc gtgggagcgg tgacccaggg caactcgagc gccatctgca aatggacacg
     1621 cgatctgtac acccaggagg ggagcgtggg ctgcgcggga ggcaccccct cgatcatgat
     1681 ggccgtgatc gaccccttcg atttcaccgc gtggacctcg cttttcaacg gtacgggaca
     1741 tcacctcggg gcatacaaca gcgcggtgtg caacaacgtg ggccatccgt tcaaggcgtt
     1801 cttctaccgg cagaacaacc cgggcgaaat ggccgcgctg gcggccaccc tgaccagtcc
     1861 cgattcgata cccgctggtc attatttgtt gttctacacc ctgaggtaca tcgatctgcc
     1921 cgccatcacc gggtccggtt tgatgaccgc gttcgccgcg atgggtgctg atacattgaa
     1981 cagcgggttg gtgcccgatc acgcgccgta catcttcttc acgcgcaagg gtgatccggc
     2041 attcgggcag gaggtgtgga gcgatgatcc gaacgccatc atcaacatcg ccgtcttcct
     2101 ggatgccaat gggaacagcg gcaccatggc caccccgcgc tccgccgaaa tgctcgattg
     2161 gaactcgctg cactggcgca tcgtgccgga acaggaacct tcggacagcg cgcggatcgt
     2221 gatccgtgcg gtggacaaca cccagcagta cgagggcccc ttctacacgg tggatcccgc
     2281 ggccaccacg gactcgctgt acttcaccga catcgggttg aacggatcgc agtacccgag
     2341 ggtgcggctc gtgggggcgt atgtgagcga gcaggacggc gatgcacgcc ccgcccagac
     2401 caagcgttgg cagatcatcg gctccccggc acccgaatgc gccatcgatc ccccgctcgg
     2461 cttcttcagt catatcgaca gcctgttcgt gggccagcag gccgaagtga tggtggccgt
     2521 acacaacatc agtgatgtgg ccatggacag cctgctgatg gccgcctggc tgacggacgg
     2581 gaacaacacc cgccacacgg tgcattggcg tatgcgggcc ccgctgcccg tgggcggcgt
     2641 actgctggac accatccggt tcgaactcca ggccggctgg ggcggaatga acgcgctcgt
     2701 catcgaggcc aaccccatcg acaccgccac cggcctgtac gaccaacgcg agcagtatca
     2761 tttcaacaac atcgccatcc tccgtttcga gaccttcgcc gaccgggaga accccgtcct
     2821 ggacgtcacc ttcgacggca tccacatcct ggatggcgac atcgtgagcg cacagccgga
     2881 agtggtgatg accctcaatg acgagaatac caccctgctc ttcgacgagg tggcggacac
     2941 ggcatatatc aaggtgtatc tgcttccccc cggcagttcc aacgagacgc tcatcccctt
     3001 tacggacccg ggtggtgccg aggtgatgcg cttcacgccc acgaacgggc cggagaacga
     3061 gtgccgcatc cagtggatgc cggatcgttt gtccgacggt gtgtaccggt tgcgcgtgcg
     3121 tgccagcgac atcagcggca accagagcgg atcccacgac ctcacggtac gcttcgaggt
     3181 ggtgaacaag cccacgatca ccgaggtgct caactacccc aatcccttca ccaccagcac
     3241 gcgcttcgtc ttcaccctca cgggcagcga aacgcccacg ggcatgcgta tacggatcat
     3301 gaccatcagt ggccgggtcg tgcgggagat catgctcgat gagctcggcc cgttgcacat
     3361 cggacgcaac atcaccgact acgcctggga cggtaccgat caattcggcg acaagctggc
     3421 gcgcggggtg tatctctacc aggtggcggc ccaattgcac ggcgaggaca tcgagcaccg
     3481 cgcgtcgggg gccgatggct ttttcaagaa gggcttcgga aagatgtatc tgctgcgatg
     3541 acgaccatgc cgggttccgc atggcctgcg ggtgaacagc gacctgttca cggagcctga
     3601 gcttgcgggt cggtggggaa ggggctccgt gtactttcgc gacgttgctc atgaatgaac
     3661 ggatcgcgcg catcggcagt tgggtggagc atctggtgaa ggcgcgcacg cggcacagcg
     3721 ttcactcgcc atttg
//
LOCUS       DAOQOC010000142         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11652_length_3730_cov_3.282177, whole genome shotgun sequence.
ACCESSION   DAOQOC010000142 DAOQOC010000000
VERSION     DAOQOC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11652_length_3730_cov_3.282177"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="PL106_03335"
     CDS             <1..1560
                     /locus_tag="PL106_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNA32131.1"
                     /translation="LFAACAQTRELSGGAKDTIAPLLLSAEPPDGTVRFANDRITLRF
                     NERVRLDRVRDRLLISPPLEKIPDVTIRAGQEVVIHLRAPLLPNTTYTFNIGDAVTDI
                     TENNSAAGLTYVVSTGNTLDSAHIAGRVIDAFTDEPEEGLMVTLHTDTDSSGFTNGRP
                     AYFTRTDKQGVFLLRHLRQGRYLLHALEDRNANLRRDLPSERLAFADAPVIAPDSTLH
                     TLRAFLPPAGIQQVKEATVQPDRAWRLVLARPADSLALWSIDRTGGDLAWAQEWNNTR
                     DTVLFWPNDTTLLNGQRFALHDRSGTIDTIAYRPLRKMPFNLDAQAAGRTEDGARLIV
                     VSRPVYRIDSARISLRIDSLTVPVRLVPVDTPRRSRVEGAPSDRSSVLTLLPGAIRDI
                     YGGTNDTLRIALGPGRPTDTGDLKINLVVDSGAVPPGPFIFQLLNTQGTVVRTRAFAT
                     LPFRVELPSVPAGAYTLKLVQDTDDDGRWSTGSLQDRRQPERTFRLAGEVNVRAGWDV
                     AVDWKVVPPLP"
     gene            1682..>3730
                     /locus_tag="PL106_03340"
     CDS             1682..>3730
                     /locus_tag="PL106_03340"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32132.1"
                     /translation="MNHPFLLRNASVTAALLLSMLGARAQTAPLASGGKDFWLGFMQN
                     AYGAQALRLTIASPSGATGTVSMPLGGWSTGFSVGPNGQTTLIVPATAEHVGSEVVAN
                     KGIRVQSDNNITVTATSYQSFTADAAQVLPLRSLGTSYRAQAYRGLPGFADFYKSELL
                     IVATEDGTQVKIVPSVNTSGGHPAGIPFTVNLDQGQTYQVQSAMAALDLTGTLVEATT
                     SSGACRPFAVFSGSMCANVPVGCPACDHIYEQMVPMDQWGTLFHTARALNVATCTIRV
                     VAAQNGTQVAVDGGAPVPLNAGQSLELNGVTSPLCISATQPVSVAQHFEGFNCAGQGD
                     PAMLELLPDERVSTSALWSTMISGQIGSHSVSVIMPTANTGQLTIDGAPVNQALFVPY
                     TGCSGWSYAAFPVASGTHRIAATGGFLAYASGTGTGESYAFIVGSVFFPDDPPQQIIC
                     SSGPVTLTAPQPIVNAEWTTASDPGTLLGTGPSLTFTPTANDTYILDGQTTNGLCPLH
                     FEWEVGLPPPSVNVTANGLASAQVCQNAAVQLNVLPVPDPGSFDLAWTPSSTMNDATV
                     PDPVAYPATDTWFKLLVTSPVGCGSVIDSVFVDVLPHDVEAIVAVALDDTVCPGQSTT
                     LQARAERIVASDNFNDAPSAIWASISGGSASALCGAITGNALRFDGAGTRSATTTPFN
                     M"
BASE COUNT          659 a         1316 c         1104 g          651 t
ORIGIN      
        1 ctgttcgccg cctgcgcgca gacacgcgaa ctttccggcg gggccaagga cacgatcgca
       61 ccactgctcc tgagcgcgga gcccccggac ggcacggtcc gcttcgctaa cgaccgcatc
      121 accctccgct tcaacgaacg ggtccggttg gaccgggtgc gcgaccgcct cctgatatcc
      181 ccccccctgg aaaagatccc ggacgtgacg atacgtgccg gccaggaggt cgtcatccac
      241 ctgcgcgccc cactgctgcc gaacaccacg tacaccttca acatcggcga tgcggtcacc
      301 gacatcaccg agaacaactc ggccgcgggc ctcacctacg tggtgtccac cggcaacacg
      361 ctcgacagtg cgcacatcgc gggccgtgtg atcgatgctt tcaccgacga gcccgaagaa
      421 gggctgatgg tcacgctgca caccgatacc gacagctcgg gattcaccaa cggccgcccg
      481 gcctacttca cccgtaccga caagcagggt gtattcctcc tgcgccatct gcgccaaggc
      541 cggtacctcc tgcacgccct cgaggaccgg aacgccaacc tgcgccggga tctgcccagc
      601 gaacggctcg ccttcgcgga tgcgcccgtg atcgcaccgg acagcaccct gcacaccttg
      661 cgcgcctttc ttccccctgc ggggatccaa caggtgaagg aagccaccgt acagcccgac
      721 cgcgcatggc gactggtttt ggcccgaccg gcggacagcc ttgccctgtg gtccatcgat
      781 cgcaccggcg gcgacctcgc ctgggcgcag gagtggaaca atacccgcga taccgtattg
      841 ttctggccga acgacaccac cctgctcaac ggtcagcggt tcgcgcttca cgacaggtcc
      901 ggcacgatcg ataccatcgc ctaccgccca ctgcgcaaaa tgcccttcaa tcttgatgca
      961 caggccgccg gacgcacgga ggacggtgcc cggctcatcg tcgtgtcgcg cccggtatac
     1021 cgcatcgata gtgcgcgcat ctccctgcgt atcgactcgc tgaccgtccc agtacgcttg
     1081 gtccccgtgg acacacccag gcgatcccgt gtggaaggtg caccaagtga tcgatcctcc
     1141 gtgctcacgc tcttgcccgg tgccatccgc gatatctatg gcggaacgaa cgacactttg
     1201 cggatcgctc tggggccggg ccgaccgact gataccggag acctgaagat caacctcgtg
     1261 gtcgacagtg gtgccgtacc ccccggcccg ttcattttcc aattgctcaa cacacagggc
     1321 accgtggtgc gcacccgggc cttcgctacc ttgcccttcc gtgtcgaatt gccttccgtg
     1381 cccgccggtg cgtacacgct gaaactcgtt caggataccg atgacgatgg tcgctggagc
     1441 accggttcgc tgcaggacag gcgccaacct gaacgcacct tccgcctggc cggtgaagtg
     1501 aacgtgcggg ccggttggga cgtggcggtg gactggaagg tggtgccacc gctgccctga
     1561 tcctgcccac acttcctgct cgccgggacc ggagcggggg ctcatcggca cgggcggccc
     1621 acccgtcgaa gaacatttga ccgtcgatcc cgtaggaagg gccgctattc ggacccgacc
     1681 ggtgaaccac ccattcctcc tgcgcaatgc cagcgtaacc gcggcactgc tcctgtccat
     1741 gcttggtgct cgtgcgcaaa cggccccatt ggccagcggc ggcaaggact tctggctcgg
     1801 attcatgcag aacgcgtacg gggcacaggc cctgcgcctc accatcgcct cgccaagcgg
     1861 cgccacgggc accgtgagca tgccactggg tggttggagc acgggattct ccgtagggcc
     1921 caacgggcag accaccttga tcgttccggc cacggccgag catgtcggta gcgaggtcgt
     1981 ggccaacaag ggcattcgcg tccaaagcga caacaatatc acggtcaccg ccaccagcta
     2041 ccagagcttc acggctgatg cggcacaggt gctgcccctg cgctccctgg gcaccagcta
     2101 ccgcgcacaa gcctaccggg gtctgcccgg attcgcggat ttttacaaga gcgagctgct
     2161 catcgtggcc accgaggacg gcacccaggt gaagatcgtg cccagcgtga acaccagcgg
     2221 tggccatccg gccgggatcc cgttcaccgt gaacctggac cagggacaga cctaccaggt
     2281 gcagagtgcc atggccgcct tggatcttac cggcacgctg gtagaagcca ccacttcaag
     2341 cggtgcgtgc cgtcccttcg cggtcttcag cggaagcatg tgcgccaacg tgccggtggg
     2401 ctgcccggcc tgcgatcaca tatacgaaca aatggtaccc atggaccagt ggggtacctt
     2461 gttccacacc gcgcgtgccc tcaatgtggc cacttgcacc atccgggtgg tggccgcgca
     2521 gaacggcacg caagtggccg ttgacggtgg cgcgcccgtg ccgttgaacg ccggtcaatc
     2581 gctcgaattg aacggcgtga cgagcccgct ctgcatcagc gccacccagc ccgtgagcgt
     2641 ggcccagcat ttcgagggct tcaactgtgc ggggcagggc gatccagcca tgctggaatt
     2701 gctgcccgat gaacgggtat ccaccagcgc actgtggtcc accatgatct ccggtcagat
     2761 cggatcgcac tccgtgagcg tgatcatgcc gaccgcgaat accgggcaac tcacgatcga
     2821 tggcgctccg gtgaaccagg ccctcttcgt gccctatacc ggttgttcgg gatggtcgta
     2881 cgccgcgttc cccgtggctt ccggcacgca ccggatcgcg gccaccggcg gtttcctcgc
     2941 ctacgccagc ggcacgggca ccggcgagag ttatgccttc atcgtgggca gcgtgttctt
     3001 ccccgatgat ccgccgcagc agatcatctg ctcctcgggg ccggtgaccc ttaccgctcc
     3061 ccagcccatc gtgaacgccg agtggaccac cgccagcgac cccggcaccc tgctgggaac
     3121 cggcccctcg ctcaccttca ccccgacagc taacgatacc tatattctgg atggtcagac
     3181 caccaacggt ctctgtccgc tgcactttga atgggaggtg ggcttacctc ccccttcggt
     3241 gaacgtgacg gccaatgggc tcgcttcggc ccaggtctgc cagaatgcgg cggtacaact
     3301 gaacgtactg ccggtccccg acccgggttc atttgacctc gcatggaccc cgtccagcac
     3361 catgaacgat gccaccgtgc ctgatccggt ggcctatccg gcgaccgaca cctggttcaa
     3421 actgctggtg acgagccctg taggctgtgg ctcggtgatc gacagtgtct tcgtggatgt
     3481 gttgccccat gatgtggaag cgatcgtggc cgtcgccttg gacgacacgg tctgtcccgg
     3541 gcagagcacc accctccagg ccagggcgga gcggatcgtg gcctcggaca atttcaacga
     3601 cgcaccgtcc gccatctggg cgtccatctc cggcggctcg gcaagtgcgt tgtgcggcgc
     3661 catcaccggt aatgcgctcc gcttcgacgg ggccggcaca cgttcggcca ccaccacgcc
     3721 tttcaatatg
//
LOCUS       DAOQOC010000143         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11686_length_3724_cov_3.680839, whole genome shotgun sequence.
ACCESSION   DAOQOC010000143 DAOQOC010000000
VERSION     DAOQOC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11686_length_3724_cov_3.680839"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PL106_03345"
     CDS             complement(<1..449)
                     /locus_tag="PL106_03345"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNA32133.1"
                     /translation="MAAGAQSSARFFRIPVGSDPLLRFLLIAAALFVGWQLLYALVIH
                     PWGVLDRWLIDRLTNNAGSLLQVFGYELLPEPSGDTNRYIGVQGGNFLWIGDRCDGLS
                     VMVVFAMFLLAFPGPWRHKPWFALLGILSIHFINVLRVAALCIVVTI"
     gene            1001..3439
                     /locus_tag="PL106_03350"
     CDS             1001..3439
                     /locus_tag="PL106_03350"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNA32134.1"
                     /translation="MAAAAGPKAGIIDVNDLRSFLRIFSKNWYFVVVALILSAVLSYL
                     YSYKIPEVYGASTQILLKDRETYNYQTQVYQNIGYIAAYGDIVNQKRVLTSYDMLNRT
                     LDKLDFDVSYYIIGRFKRKEEYGTLPFTVKMQLLNPKLFEKPFDLHIVDPEHFELSYD
                     RGGGIIKKTFPFNTDVADLADDDFTLRVDKVQEVTASNLERYTSGDYQFVVHSRGWLV
                     GKYSYGMQVENLEYTSILEVRVEDEIPARARMLLDTLSREYINYTLQSEFDINERTLS
                     YIDKQIDEVSSIISMYEDDLEQFVNSKNILDLNKQEDQYFDQLVSYDAKKRQYEMMIE
                     SVNSLEEYVLDLGEEKLLPPSLYILEDDIFLKQTLSELYNMQMAKNNKLFDATMGNPS
                     ISRGDTTLALNRANLMVYLTNTRKALNEKIDEVQGQIDDYTRLIKGVPSTQRGLLNIR
                     RKLNVNEKLYEFLLEKRANTMIAKAGIIPTTKVIERARSLGVVRPDRMKILYTFLVGG
                     LLLSMVVVFIRVLFYDRVENADQLRAATSLPVFGEIIASEKAEENYVVVDSDPKSAIT
                     ESFRTVRTNLEYLPASSQGKVVMVTSYRPNEGKTFCSVNLSAILAKAGKKVLLLELDL
                     HKPKVGANMGMTAQQGLTNILVGKIPVGECVQPTQIENFNVILSGPTPPNASELVLSK
                     HLAELFEWGRTRFDYVIVDTPPVGLITDALLMMRHVDATLFVINTRFANKDHIRNAVE
                     TAHASTTKNFGFILNGVRMKKSRYYYNTNYGYGYRYAYGYGSGYGYGYGYGQGRRKKK
                     GDAEGGGKAKNQNT"
     gene            3475..>3724
                     /locus_tag="PL106_03355"
     CDS             3475..>3724
                     /locus_tag="PL106_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32135.1"
                     /translation="MNGPGTSAWDVTIKPQQAWWRPDLKEFWHYRDLLMLLVRRDLTA
                     QYKQTILGPAWLVAQPLLTTITYTVMFGLVARLAPAGIP"
BASE COUNT          883 a         1104 c         1148 g          589 t
ORIGIN      
        1 tcgatggtga ccacgatgca aagggccgcc acgcgcagga cgttgatgaa gtggatggag
       61 aggatgccga ggagggcgaa ccagggctta tggcgccagg gaccggggaa ggcgagcagg
      121 aacatggcga agaccaccat cacggaaagg ccatcgcacc ggtcgccgat ccataggaag
      181 ttgccgccct gcacgccgat gtagcggttg gtatcaccgg aaggctcagg caggagctcg
      241 taaccgaaca cctgcagcag gcttccggca ttgttggtca gccgatcgat gagccaccgg
      301 tccagcacac cccacgggtg gatgaccaag gcgtacagca attgccaccc cacaaagagg
      361 gccgcagcga tgaggaggaa gcggagaagc ggatcggagc cgaccgggat ccggaagaac
      421 cgggcggaag actgggcacc agcggccatc aggccgtgcg cttggacgcc tggcgcagat
      481 caagggcgcg cttggcgccg aggagcacac cagctccaat gagcagagag atccccccgt
      541 caacggggat gcagggaggg ggccagcagg gaggagggcc acccacgggc gggccaccct
      601 gtgcccaaag cccaccgatc aaggggaggg ccagcgagcc tgcgagtacc accaagcgta
      661 ggactgactt catgctttcg gagcgctcag aacggggcca aatgtagaaa gatcgtcgga
      721 tgtgcttcta cggggcacga accattgttt tcaacgatcc attcgctcag cgaaaaggag
      781 gcgatcccga cgcgatccat cagcacagca ggtccggatg cgtaccgcga tcctccatac
      841 gggagcctgt tcggctcccc acgaacccgc gcagcccagc acgtatggga agctctcgcc
      901 gaagtggcgc cggccaggag cccgaatgcg cggcctatat tcgcagccct ttcaaaacag
      961 gggtccgagg gccggtccgg ccatcacccg cacggagacc atggcagcag cggcaggccc
     1021 caaggcgggc atcatcgacg taaacgacct gcgctccttc cttcggatct tctcgaagaa
     1081 ctggtacttc gttgttgtcg ccctcatcct ctcggccgtc ctctcctacc tgtacagcta
     1141 caagataccc gaggtgtacg gcgccagcac gcagatcctg ctgaaggacc gcgaaacgta
     1201 caactaccag acccaggtgt accagaacat cgggtacatc gcggcctacg gtgacatcgt
     1261 gaaccagaag cgggtgctaa cgagctacga catgctcaac cgcacgctgg acaagctgga
     1321 cttcgacgta tcatactaca tcatcggtcg cttcaagcgc aaagaggaat acggcacgct
     1381 gcccttcacg gtgaagatgc agttgttgaa ccccaagctt ttcgaaaagc ccttcgacct
     1441 gcacatcgtc gacccggaac atttcgagtt gagctacgac cggggcggcg gcatcatcaa
     1501 aaagaccttc ccgttcaaca ccgatgtggc cgacctggcg gacgatgact tcacattgcg
     1561 cgtggacaag gtgcaggagg taacggcatc gaatctggaa cgctacacca gcggcgatta
     1621 ccagttcgtg gtacacagcc ggggctggtt ggttggcaag tacagttacg gcatgcaggt
     1681 ggagaacctg gagtacacct ccatcctgga agtgcgtgtg gaggacgaga tccccgcgcg
     1741 tgcccggatg ttgctggaca cgttgagccg ggagtacatc aactacacgt tgcagagcga
     1801 gttcgacatc aacgaacgca cgctcagcta catcgacaag cagatcgacg aggtgagttc
     1861 catcatctcg atgtacgagg acgatctgga acagttcgtg aacagcaaga acatcctgga
     1921 tctgaacaaa caggaggacc agtatttcga tcagctcgtg agttacgacg ccaagaaacg
     1981 gcagtacgag atgatgatcg agagcgtgaa ctccctggag gagtacgtgc tggaccttgg
     2041 cgaagagaag ttgctgccac cgagcctgta catcctggag gacgacatct tcctgaagca
     2101 gaccctgagc gagctgtaca acatgcagat ggcgaagaac aacaagctct tcgatgccac
     2161 aatgggcaac ccctcgatca gccggggaga taccacgctg gccctcaacc gggccaacct
     2221 gatggtgtac ctgaccaata cacgcaaggc cctgaacgaa aagatcgatg aggtgcaggg
     2281 ccagatcgat gattacaccc gtttgatcaa aggagtgccc tccacccaac ggggcctgct
     2341 caacatccgg cgcaagttga acgtgaacga aaagctctac gagttcctgc tggaaaaacg
     2401 ggccaacacc atgatcgcca aggcgggcat cattcccacc accaaggtga tcgagcgcgc
     2461 gcgatcgctg ggcgtggttc ggccggaccg gatgaagatc ctgtacactt tcctggtggg
     2521 cgggcttttg ctgagcatgg tggtggtctt catccgggta ctgttctacg accgggtgga
     2581 gaacgccgac caattgcgcg cggccaccag tctgccggta ttcggggaga tcatcgccag
     2641 cgagaaggcc gaggagaact acgtggtggt ggacagcgac ccgaagagcg cgatcaccga
     2701 gagcttccgc acggtgcgga ccaacctgga gtacctgccg gccagctcgc agggcaaggt
     2761 ggtgatggtg acgagctacc gtccaaacga gggcaagacc ttctgcagcg tgaacttgag
     2821 cgcgatcctg gccaaagcgg gcaaaaaagt gctcctgctg gagttggacc tgcacaagcc
     2881 caaggtgggg gcgaacatgg gcatgaccgc ccagcaaggc cttacgaaca tcctggtggg
     2941 caagatcccc gtgggcgagt gcgtgcagcc cacacagatc gagaacttca acgtgatcct
     3001 gagcggcccc accccgccga acgcctcgga actggtcctc agcaagcact tggccgagct
     3061 ctttgaatgg ggccgtacac gattcgatta cgtgatcgtg gacaccccgc cggtggggct
     3121 gatcaccgat gcgctgctga tgatgcgcca tgtggacgcc accttgttcg tgatcaacac
     3181 ccgattcgcc aacaaggacc acatccgcaa cgcggtggaa acggcccacg ccagcaccac
     3241 caagaacttc ggcttcatcc tgaacggtgt ccggatgaag aagagccgct attactacaa
     3301 caccaactac ggatacggct accgctacgc atacgggtac ggtagcgggt acggctacgg
     3361 ctacggctac ggacaaggcc gccgcaagaa gaagggcgac gcggagggcg gtggcaaggc
     3421 gaagaaccag aacacctgag acggaacgga gcggccacgg ggccggccat ggacatgaac
     3481 ggacctggaa ccagtgcgtg ggatgtcacg atcaagccgc agcaggcctg gtggcgaccc
     3541 gacctgaagg aattctggca ctaccgcgac ctgctcatgc tgctggtgcg gcgcgacctc
     3601 acggcgcagt acaaacagac catcctggga ccggcctggt tggtggccca gccgttgctc
     3661 accaccatca cctacaccgt gatgttcggt ctggtggcac gattggcccc ggcgggcata
     3721 cccg
//
LOCUS       DAOQOC010000144         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11892_length_3686_cov_3.339025, whole genome shotgun sequence.
ACCESSION   DAOQOC010000144 DAOQOC010000000
VERSION     DAOQOC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_3686_cov_3.339025"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..635)
                     /locus_tag="PL106_03360"
     CDS             complement(66..635)
                     /locus_tag="PL106_03360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA32136.1"
                     /translation="MNRILFLPLVITATLSAFSQQERLLNVQVGQMAPEIVMANPSGD
                     VLRLSELKGKLVLVDFWASWCRPCRMESPHVRRAYHTYKDRLFTKGDGFAVFSVSLDR
                     QGQAEAWKAAIAKDSLDWKWHVGAVNEVQNAAAEEYGARFIPTNALVDGEGRIVAKDL
                     HGEALTNALNALLEKDPMKLERHGKKAKR"
     gene            1008..1751
                     /locus_tag="PL106_03365"
     CDS             1008..1751
                     /locus_tag="PL106_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32137.1"
                     /translation="MKRTATLLCSALITATTFAQNWVVDVPVDMLLYTQPFFGGCTPG
                     PDYTFTMPASPVSGVQYKVVITAMDPETGSLSISPGLDNGLLHDELVFDAAVERTVTF
                     AAGTTSATLEFRAQGIPTEAGQPHPCGPSAFWMSNLMFCPEGLTPNFDAGCTVQSDAT
                     LITDITASGPTIGLPTALNGQHLSIGLPTHGTAQVFIIDASGRMVCSRVLSNGGAIDL
                     SGLTDGAYMLRLVRSTGGVLTKRFLMSSR"
     gene            1777..3405
                     /locus_tag="PL106_03370"
     CDS             1777..3405
                     /locus_tag="PL106_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32138.1"
                     /translation="MRSTLLILFVGLGVGALAQDPMRSGVAYSKSIKGVEGDGQLVAN
                     ATTDLNGMPVLFMMEGKGYKAVRLGADLRPAEEIPLDLQTFDGAVWDAASSIVVDDKL
                     HILFVSNGKKTADYGIGRLNTEGALAIQDFHRVASFDHPNTFDPSTTTCRKTLPDLIL
                     FDSGAFYDQGDRIVASPDGKHFLVNHYTSGGKAKKKFWFACLDRSFQKEWSGSIELPF
                     EDANSDIHQIVLDNSGKIILLTYVFCGDLARQADKLCHETHLTVLKDQGTRLKDLLLD
                     KDFVSSARVMPKDDGRILVAIRYGALTGIPGVVVSIDTAMAKLKNTPVVDQRVPGIRK
                     TKLATFGLPDDGSGKKPSGSRAAKVPDEIIELTPAWGGTLLLEGFRDQDLEVVMGDAI
                     AMRTLHGAVRATWLDATDSIRWQRTVDRAFMTTSGESYGSVAFALLPEGLLLAYNDTP
                     GGIAAINNSYTGASGKKKEKVPVEKSRLKLAWIPAQGGTPKEEAVGTPEGDFTLCPMT
                     MVRSRAGDKLWIKGYDRGSQHQFVQVDPRVLERE"
     gene            3487..>3686
                     /locus_tag="PL106_03375"
     CDS             3487..>3686
                     /locus_tag="PL106_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32139.1"
                     /translation="MNERTEDPRPPRWQRRAQLRLTGALAWLGTKARNTRLKELTLLT
                     LPYQVAAVITALIAVGYAKLFE"
BASE COUNT          725 a         1116 c         1120 g          725 t
ORIGIN      
        1 tcaggtgcta aaataggtgc tggatcggat gcgccttgag cgaacatcgt gccggatcag
       61 gacggctacc gcttggcctt cttcccgtgc cgttccagct tcatcggatc tttttccagc
      121 agggcgttca atgcgttggt aagggcttcg ccgtgaaggt ccttggccac aatgcgaccc
      181 tccccatcaa ccagggcgtt cgtggggatg aaccgggctc catactcttc ggcggccgcg
      241 ttctggacct cgttcaccgc acccacatgc catttccagt cgaggctgtc cttggcaatg
      301 gcggccttcc atgcctccgc ctggccctga cggtccaggc tcacactgaa gaccgcgaaa
      361 ccgtcacctt tggtgaagag acggtccttg tacgtgtggt acgcacggcg cacgtggggg
      421 ctttccatac ggcagggacg gcaccaactg gcccagaaat ccaccaggac cagcttcccc
      481 ttcagttcgg aaagccgcag gacatctccg ctggggttgg ccatcacgat ctcgggcgcc
      541 atttggccca cctgaacatt gagaagtcgc tcctgttggc tgaaggcgga cagggtggcg
      601 gtaatgacca aggggaggaa aagtatccga ttcatgtggc gaagatcgga agcggcgagc
      661 aaggatcggg ccgtattctg ccagcaggtg atggtggctg aacgcgatcg agtgatacgc
      721 ctggtctgga cgccattgga acaccgacgg atccagttcg ttgcttcctt ggagacgtgc
      781 agtgaatttc tcaaaatgca gcaggaagtg agcggggcgt atgttgcgtt catgcgaggc
      841 gccggaaccg ggcatggtcg ggcacactgt gaggttccgt caacggaaca tggaccttag
      901 cgacccacgt gcggcccgaa gtggatcttt gaaatgtgct ctattcccag aaggtcggtg
      961 ctggatcggc ctgctcatgc ctagtttcac cacccaactg aacccccatg aaacgaactg
     1021 ctacgctcct gtgctccgcg ctgatcactg cgaccacctt tgcccagaac tgggttgtgg
     1081 acgtgccagt ggacatgctg ctgtacaccc aacctttttt cggtggctgc accccggggc
     1141 ccgactacac ctttaccatg cccgccagcc cggtgagtgg cgtgcagtac aaagtggtca
     1201 tcacggccat ggatccggaa acgggcagcc tgagtatttc gccgggcctg gacaacggcc
     1261 tcttgcatga cgagttggta ttcgacgcgg ccgttgagcg caccgtgacc ttcgctgccg
     1321 gtaccaccag tgccacgttg gagttccgtg cccaaggcat acccacggag gcgggccagc
     1381 cacacccctg tggtccgagc gccttctgga tgagcaattt gatgttctgc ccggaaggat
     1441 tgacacccaa tttcgatgcg ggatgcaccg tgcagagcga tgcgaccctg atcacggaca
     1501 tcacagcgtc cgggccgacg atcggcctgc ctaccgcgct caatggtcag cacctcagta
     1561 tcggcctgcc aacacatgga actgcccagg tgttcatcat tgatgcgagc ggacgtatgg
     1621 tctgctcccg ggtactctcc aacggaggtg ccatcgacct ctcgggcctg accgatggcg
     1681 cgtacatgct gagactcgtc cgttccacgg gtggggtgct cacgaagcgg ttcctgatga
     1741 gcagtcgata agggatccgc gatcatcagc cccaccatgc gctccacact gctcattctg
     1801 ttcgtaggcc tcggcgttgg cgccctcgcc caagacccca tgcgctccgg tgttgcctat
     1861 tcgaagtcga tcaaaggagt cgagggcgat ggacaactcg tggccaacgc caccaccgac
     1921 ctgaacggca tgcccgtgct cttcatgatg gagggcaagg gctacaaagc ggtgcgcctt
     1981 ggtgccgacc tgaggccggc cgaagagatc ccactggacc tgcagacctt cgatggtgcg
     2041 gtgtgggatg ccgcttcgtc gatcgtggtg gacgacaaac tccacatcct tttcgtgtcg
     2101 aacgggaaaa aaaccgcgga ttacgggatc gggcgtctga ataccgaagg tgccctcgcc
     2161 atccaggatt ttcatcgggt ggcctcattc gatcatccga acaccttcga tccgagcacc
     2221 acgacctgcc ggaaaaccct gcccgacctg atccttttcg acagtggcgc attctacgac
     2281 cagggcgacc gaattgtcgc ttcgccggat ggcaagcact tcctggtgaa ccattacacc
     2341 tccggcggga aagcgaaaaa gaaattctgg ttcgcctgct tggacaggtc attccagaag
     2401 gaatggagcg gttcgatcga actgcccttc gaggatgcga acagcgacat ccaccagatc
     2461 gtgctggaca atagcggaaa aatcattctt ctaacgtacg ttttctgcgg cgatcttgcg
     2521 cgccaggccg acaaactctg ccacgaaacg cacctcaccg tcctcaagga tcagggcacg
     2581 cggttgaagg acctgctgct cgacaaggat ttcgtttcca gcgcccgcgt gatgcccaag
     2641 gatgacggga ggatccttgt ggccatccgt tatggggcgc tcacgggcat ccccggggtc
     2701 gtcgtgagca ttgataccgc gatggccaaa ttgaagaaca cccccgtggt ggaccagcgt
     2761 gtgccgggga tccgaaagac gaaactcgcc accttcggcc tgcccgatga cggcagcggc
     2821 aaaaagccat cgggttcgcg cgcggccaag gtgcccgacg agatcatcga actgacgccc
     2881 gcttggggcg gtacgttgtt gttggaagga ttccgggatc aggatctcga ggttgttatg
     2941 ggcgacgcta ttgcgatgcg cacgctgcac ggagctgttc gcgccacatg gctcgatgcc
     3001 accgacagta tccgctggca gcggaccgtg gaccgtgctt tcatgaccac atcgggagag
     3061 tcctatggca gcgtcgcttt cgcactgctc cccgaagggc tcctgctcgc ttacaacgat
     3121 acgccgggtg ggatcgctgc gatcaacaac agttacaccg gggccagcgg caagaagaag
     3181 gagaaggtgc cggtggagaa aagccggttg aaactcgcat ggatccctgc acagggaggc
     3241 acaccgaagg aggaggccgt gggtacaccc gagggcgact tcaccctctg ccccatgacc
     3301 atggtgcgtt cccgtgcggg cgataagctc tggatcaagg ggtacgatcg gggcagtcag
     3361 catcagttcg tgcaggtgga tccgcgtgtg ttggagcgcg aatagggccg ttgtcagtcc
     3421 gtccttgttg gtagcacctg cgcgcggtgc cgcatcccgg tgtgttgccc gcgcattttc
     3481 gcggccgtga acgagcgtac cgaggatccc cgtccgccgc gttggcagcg gcgtgcccag
     3541 ctccggctca ccggcgcgct cgcctggctg ggtaccaagg cgcgcaatac caggctcaag
     3601 gaactgaccc tcctcacgct gccgtaccag gtggccgccg tgatcacggc actcatcgcc
     3661 gtgggctatg ccaagctctt cgagta
//
LOCUS       DAOQOC010000145         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_11933_length_3679_cov_3.725166, whole genome shotgun sequence.
ACCESSION   DAOQOC010000145 DAOQOC010000000
VERSION     DAOQOC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11933_length_3679_cov_3.725166"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PL106_03380"
     CDS             complement(<1..1961)
                     /locus_tag="PL106_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNA32140.1"
                     /translation="MEKFISDITYKAVDFDPFGGPAIERTVPSTEAQREVFVASLMGP
                     EASCAYNESVSLELTGELDVARMEQVIAQLVARHESLRCRMSADGLRIIVLEHEDIKH
                     PFIDLTGKNAIEQQRELDAFAERDMTTAFDLLNGPVFRTVIIKLAIDKHLLRLTGHHL
                     VCDGWSLGIIMADISKLYNGDPLEAAVPFSDYAMAQLDFEQSAAQAEVERYWLDTFKG
                     TLPRVDLPTDRPRPQQKTFSSRRIDMEMDPGLVRRLRETATRTGTSLVTTLLTCFELL
                     VYRLTGDGDMCIGLPAAGQNDFGMKNLVGHCVNLLALRSRVDDERPFAEHLKERRSTM
                     LDVSDHQKYTFGTLVRKLNVPREPGRVPLVPVVFNIDMNMDDGVAFKGLTHRLVSNPR
                     RFENFELFLNIVGRRDGGMGIEAQYNTDLFDDATIRRWLGLYETLMHSAMDDLSRPVG
                     QLALLPSSELQALAALQPTPVPAEGVPLMHAGFTRQAKATPERAALRSGRERMSYREL
                     DERSNQLAHALRTRGIGRGQHVGLCLDRGIDMMVALLAVLKSGAAYVPLDPGFPQARL
                     DYYAEDARLALLLTDSRVTSAPRAWCVDAEKRVLLMDRDTAWRSGPVDVLAPDAKLDA
                     GPEDAAYVIYTSGSTGKPKGVRLPHRAVA"
     gene            complement(1998..>3679)
                     /locus_tag="PL106_03385"
     CDS             complement(1998..>3679)
                     /locus_tag="PL106_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA32141.1"
                     /translation="DAITDNKHASSTAKDGGVPANALEDAPELKKAFGAQARISKEKV
                     DDMTPQQRAWFDAFVQRYTAKTAKSKAFTQENRKPMADPRVVTGFKPQTKELIYQVVV
                     DKSTGCHLLDIDGNEYVDILSGFGSSMFGYMPDFIKEAVHKQVDTSVEIGPMHPLAAE
                     VSKLLCELTGAERAAVCNTGSEAVLGAMRMARTVTGRSLIISFSGSYHGINDEVIIRG
                     SKSKKSYPGAPGIMPEAVQNMLVLDYGTPESLEIIKQRCHEAAAVLVEPVQSRRMEFR
                     PVDFLRELRRITKEHGTALIFDEVITGFRTHQNGTQGLFGIQADIGTYGKVIGGGMPV
                     GAMIGKSEWMDALDGGHWRYGDDSVPPAGVTYFAGTFVRHPLTMAAMKASLVYMKNEG
                     PALQERLNTVTEGMVARVNGLFDKYQLPYRWVNFGSAFKTKYDESVNYTELFFMLMRY
                     HGVHVLDFPHFITTAHTAADIEHIIAAVEKTCKELRESGFMPERTYPIPYVNSVLNGH
                     ARKLSERVIKASEPPVPHARLGRTPKGDPAWFVPDPQRAGRYLMITNEADI"
BASE COUNT          616 a         1160 c         1150 g          753 t
ORIGIN      
        1 ttggccacag cgcggtgcgg aaggcgaaca cccttgggct tgcccgtgga acccgaggta
       61 tagatgacgt atgcggcatc ttccggcccg gcatccagct tcgcgtcggg tgccaacaca
      121 tccaccggac ccgacctcca ggccgtgtcc cgatccatga gcaatacccg tttttccgca
      181 tcgacgcacc aggctcgtgg tgccgatgtc acacgggagt cggtgagcag cagggccagt
      241 cgggcatcct ccgcgtaata gtccaaccgc gcctggggga atcccgggtc gagcggaacg
      301 taggccgcac cgctcttgag cacagccagc aacgccacca tcatatcgat cccgcgatcc
      361 aggcatagtc ctacatgctg gccacgaccg atgccacgcg tacgcagtgc atgggccagt
      421 tggttcgagc gctcgtccag ttcgcggtag ctcatccgct cccggccact gcgcaaagct
      481 gcacgttcgg gggtggcctt ggcctgtcgg gtgaagcccg cgtgcatcaa cgggacgcct
      541 tcggccggca ccggagtcgg ttgcaatgcg gcaagcgctt gcagctcgga cgatgggagg
      601 agcgcgagtt ggcctacggg ccgcgacagg tcgtccatcg cgctgtgcat caaggtctcg
      661 tacaggccca gccaacgccg gatggtggcg tcgtcgaaca ggtcggtatt gtattgcgct
      721 tcgatgccca tgccaccgtc gcgcctcccg acgatgttca ggaacagctc gaagttctcg
      781 aaacggcgcg gattgctgac gagccggtgg gtgaggccct tgaaggccac cccatcgtcc
      841 atgttcatgt cgatgttgaa cacgacgggt accagcggca cacggcccgg ttcgcggggc
      901 acgttcagct tgcggaccag cgttccgaag gtatatttct ggtggtcact gacatcgagc
      961 atggtgctgc gccgttcctt caagtgctcc gcgaagggtc tctcatcatc cacccggctg
     1021 cgcaaggcca gcaggttcac gcagtgcccc accaggttct tcatcccgaa gtcgttctgt
     1081 ccggcggcgg gcaggccgat gcacatgtca ccgtcgcccg tcaaccggta cacgagcagc
     1141 tcgaaacagg tcaacagcgt ggtcaccagg ctcgttccgg tgcgcgtggc ggtttcgcgc
     1201 agtcgacgca cgagcccggg atccatctcc atgtcgatgc gtcggctgct gaaggtcttc
     1261 tgctgcggac gcggccggtc ggtgggcagg tccacccggg gcagcgtgcc cttgaaggtg
     1321 tccagccaat aacgctccac ctccgcctgc gcggcactct gttcgaagtc gagctgcgcc
     1381 atggcgtaat cgctgaaggg caccgcggcc tccagtggat cgccgttgta cagcttgctg
     1441 atgtcggcca tgatgatgcc cagactccag ccatcgcaca ccaagtggtg cccggtcagg
     1501 cgcaacagat gcttgtcaat ggccagcttg atgatgacgg tgcggaacac ggggccgttc
     1561 agcaggtcga acgccgtggt catgtcacgc tccgcgaagg catccaattc gcgctgctgt
     1621 tctatggcgt tcttcccggt caggtcgatg aacggatgtt ttatgtcctc atgttcaagt
     1681 acgatgatgc gcaggccatc ggcgctcatg cggcaacgca gtgattcgtg ccgtgcgacc
     1741 agctgcgcta tcacctgctc catccgggcc acatccagct cgccggtgag ttccagggag
     1801 acggactcgt tgtaggcgca tgaggcttcg ggacccatca gcgaggccac gaacacttcc
     1861 cgctgggcct cggtgctcgg gaccgtccgc tcgatggccg gccctccgaa cggatcgaag
     1921 tccaccgcct tgtacgtgat atccgaaatg aatttttcca tgcttgcgtt cactgtgcgc
     1981 ggagcggcgc tgtggcgtca aatatctgct tcgttggtga tcatcaagta cctgccggcc
     2041 cgctgcggat ccggtacgaa ccacgcggga tcgcccttgg gtgtgcggcc gagccgtgcg
     2101 tgtggaacgg gaggctcgct ggccttgatc acacgttcgc tcaacttgcg tgcgtggccg
     2161 ttcagcacac tgttgacata cggtatcgga tacgtccgct ccggcatgaa gccgctctca
     2221 cgaagttcct tgcaggtctt ctccaccgca gcgatgatgt gctcgatgtc ggccgccgtg
     2281 tgcgccgtgg tgatgaagtg cgggaagtcc agcacatgca cgccatggta tcgcatcagc
     2341 atgaagaaga gctcggtata attgacgctc tcgtcgtact tcgtcttgaa ggcgctgccg
     2401 aagttcaccc agcggtaggg gagctggtac ttgtcgaaga gaccgttcac gcgcgctacc
     2461 atgccctcgg taacggtgtt caggcgctcc tgcaaggccg ggccttcgtt cttcatgtag
     2521 accagcgagg ccttcatggc ggccatcgtc agtgggtggc gcacgaaggt gccggcgaag
     2581 taggtcacgc ctgcgggcgg aacgctgtcg tcgccgtatc gccaatggcc gccatcgagg
     2641 gcatccatcc attcgctctt gccgatcatg gcgcccacgg gcatgccgcc gccgatcacc
     2701 ttgccgtagg tgccgatgtc cgcctggatg ccgaacaatc cctgcgttcc gttctggtgt
     2761 gtgcggaagc cggtgatcac ctcatcgaag atcagggctg tgccgtgttc cttggtgatg
     2821 cggcgcaatt cgcggaggaa gtccaccgga cggaactcca tgcgtcggct ttgcaccggt
     2881 tccaccagga ccgcagcggc ctcgtggcag cgctgcttga tgatctccag gctctcgggc
     2941 gtgccgtagt cgagcaccag catgttctgc acggcctcgg gcatgatgcc gggcgcgccg
     3001 ggatagctct tcttgctctt gctgccacgg atgatcacct cgtcgttgat gccgtggtag
     3061 ctgccgctga aggagatgat caggctgcga cccgtcacag tgcgggccat gcgcatcgcg
     3121 ccgagcaccg cttcggaacc cgtgttgcag acggccgcgc gttcggcccc cgtcagttcg
     3181 cacagcagct ttgatacttc cgcggcgagc gggtgcatcg gcccgatctc cacgctggtg
     3241 tccacttgct tgtgtacggc ctccttgatg aagtcgggca tgtagccgaa catcgaggag
     3301 ccgaaaccgc tgaggatgtc cacgtactcg ttgccgtcga tgtctaacaa gtggcagccg
     3361 gtgctcttgt ccaccaccac ttggtagatc agctccttgg tctgcggctt gaaaccggtg
     3421 accacgcgcg gatcggccat cggcttgcgg ttctcctggg tgaaggcctt gctcttggcg
     3481 gtcttcgccg tgtaacgttg cacgaaggca tcgaaccacg cacgctgctg cggtgtcatg
     3541 tcgtccacct tctccttgct gatgcgggcc tgggcaccga aggctttctt cagttcaggg
     3601 gcatcctcca acgcgttcgc tgggacaccg ccatccttgg cggtgctgga tgcatgcttg
     3661 ttgtccgtga tcgcatcac
//
LOCUS       DAOQOC010000146         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12035_length_3660_cov_3.695146, whole genome shotgun sequence.
ACCESSION   DAOQOC010000146 DAOQOC010000000
VERSION     DAOQOC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12035_length_3660_cov_3.695146"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PL106_03390"
     CDS             <1..520
                     /locus_tag="PL106_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNA32142.1"
                     /translation="ESFLGNVRTLLATDEVDKAIGACDEQQGSVANVMRSGLEKYKHV
                     LFDASHDKETRLQAVKQELEEATALELPMLSKNLVILSTCASISTLLGLIGTVLGMIR
                     SFSALATAGTPDATALSTGISEALINTALGITGSCIAIIMFNYFSTKIDAITHGIDEA
                     GFSVSQTLATHK"
     gene            600..1229
                     /locus_tag="PL106_03395"
     CDS             600..1229
                     /locus_tag="PL106_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNA32143.1"
                     /translation="MPKIKMPRSSPSLDMTPMVDLAFLLVTFFMLTAQFRPEEAVSVD
                     TPSSMSQSPIPTENLMTIIVDSTGRVFWDFTDKKVRTEVIKQLASKYNFQLNEEQLTR
                     FTNLNALGMPAQLLPQYLDLNTPAERKEMDLQHKGIPVDSTNNQLFTWISITQQIFSQ
                     EAGRNPLVALKGDGNVHYNVMNQVIKTFQSPGIKINRFKMITDLEESRM"
     gene            1295..1921
                     /locus_tag="PL106_03400"
     CDS             1295..1921
                     /locus_tag="PL106_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNA32144.1"
                     /translation="MAEVPGGEGGGGGGRHQKKRAKKGSTRIDMTPMVDLAFLLLTFF
                     ILTTTMYKPSTLQLTYPVPPDEQMPQPPKKVNNAITLFLTKKDQILYYKDAFKPGETQ
                     LTRTDFKDIRKTLVELNRASVDRINQLTREFNEGKINEVVFDSLKNMSQKQEDALYVI
                     IKPDPDARFRNVIDIVDEMDICGVGKFAVQDVIKPEEMALVDIEKSKF"
     gene            1927..2766
                     /locus_tag="PL106_03405"
     CDS             1927..2766
                     /locus_tag="PL106_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNA32145.1"
                     /translation="MTAIIAVVALLMMSLLLSVDNSWMNVLFGGRNNLVFARHNREYG
                     AYELRQGYNKRLGIALVAGIGFVVLALGGTWASGKFGGEEEVEVKKKIVDVNMELFEE
                     EKEDEPEEIKEPVLVEQPKIETVQFVAVEAADEKVEEPPPTQSDVAETVVSTVTQEGE
                     NVDVAPPPAPVEEETFDLAAVQEQPEFPGGMEKMYEFMQRTQKYPDMEYEAGIQGKVF
                     VQFVVDKDGSIEEVKVLRAVSDGLDKEALRLVKSMPKWSPGKMGGKPVKCRFNLPVVF
                     KLK"
     gene            2956..>3660
                     /locus_tag="PL106_03410"
     CDS             2956..>3660
                     /locus_tag="PL106_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32146.1"
                     /translation="MLTIALFLLFALLCAALSVDRTWDSETMSERNQLVFAGRNHDYG
                     AFVLRREYDRRFLLAFVGAIALFGALVAVPKVLGSMGVWSVPAPPPVPYIPDERVMEW
                     FTTPENKPNDPRPPAPRTTTAPPTTSDNDNVTVVPKDSMPPDVPKDTLRVDPEPVTGN
                     PGPIGLAGAGPVGPGTNGAGGENGDGPAFSLDNPGNPGIVDHQPEFPGGQEALARFIQ
                     NNLVVRDEGISSARTQV"
BASE COUNT          830 a         1105 c         1121 g          604 t
ORIGIN      
        1 cgagagtttc ctcgggaacg tgcgcacact gctcgccacg gacgaggtgg acaaggccat
       61 cggtgcatgc gacgagcagc agggcagtgt ggccaatgtg atgcgcagcg gcctggagaa
      121 gtacaagcat gtgctgttcg acgcgagcca cgacaaggag acgcgcctgc aggcggtgaa
      181 gcaggaactg gaggaggcca cggcactgga actgcccatg ctgagcaaga acctggtgat
      241 cctctccacc tgcgccagca tcagcaccct gttgggtctg atcggaacgg tgctggggat
      301 gatccgctcc ttcagcgcgc tggccacggc cggcacgccc gatgccaccg ccctgtcaac
      361 cggtatctcc gaggccctga tcaacacggc cctcggtatc accggctcgt gcatcgccat
      421 catcatgttc aactacttca gcacgaagat cgacgccatc acccacggga tcgacgaggc
      481 cggcttcagc gtgagccaga ccctggccac acacaaatga ccggcagggg cgacactccc
      541 gttgcccggc gataaaatac cggacgacgg gaacgtagcg ccgacaaacc ccaagcacga
      601 tgccgaagat caagatgccc cggagcagtc cttcactgga catgactccc atggtggacc
      661 tggccttcct gctggtgacc ttcttcatgc tcaccgcgca attccgtccg gaggaggccg
      721 tgtcggtgga tacgcccagc agcatgagcc agagcccgat ccctacggag aacctcatga
      781 ccatcatcgt ggacagcacc gggcgggtgt tctgggactt caccgacaag aaggtacgga
      841 cggaagtgat caagcaactg gcgtccaagt acaatttcca gctcaacgag gaacagttga
      901 cgcggttcac caacctgaac gccctgggca tgcccgccca attgttgccc cagtaccttg
      961 acctgaacac cccggcggag cgcaaggaga tggacctgca gcacaagggc atccccgtgg
     1021 acagcaccaa caaccagctc ttcacgtgga tcagcatcac ccagcagatc ttcagtcagg
     1081 aggccggacg caacccgctc gttgccctca agggcgacgg caacgtgcat tacaacgtga
     1141 tgaaccaggt gatcaagacc ttccagagtc ccgggatcaa gatcaaccgg ttcaagatga
     1201 tcaccgacct ggaggagagt cgcatgtgaa ttgaaccggt ggtcggacgg ggtgtacagc
     1261 accctgcgac ggcgaccgga cagaacggaa caccatggca gaagtacccg gaggagaagg
     1321 cggcggcgga ggtggaagac accagaagaa gcgcgccaag aagggcagta cacgcatcga
     1381 catgacgccg atggtggacc tggccttcct gctgctcacg tttttcatcc tcaccaccac
     1441 catgtacaag ccgtcaacac tgcagttgac gtatcccgta ccgccggacg agcagatgcc
     1501 gcagccgccc aagaaggtga acaacgccat cacgctcttc ctgaccaaaa aggaccagat
     1561 cctctattac aaggatgcgt tcaagccggg cgaaacgcaa ttgacacgca cggacttcaa
     1621 ggatatccgc aagacgctgg tcgaattgaa ccgcgcctcc gtggaccgca tcaaccagct
     1681 aacacgcgag ttcaacgaag ggaagatcaa tgaggtggtc ttcgattcgc tcaagaacat
     1741 gtcccagaag caggaggatg cgctgtatgt gatcatcaag cccgatccgg acgcgcgatt
     1801 ccgcaacgtg atcgacatcg ttgacgagat ggacatctgc ggggtgggca agttcgcggt
     1861 gcaggatgtg atcaagccgg aggagatggc tctggtggac atcgaaaaat caaaattctg
     1921 acgcacatga cagccatcat cgccgtcgta gcactgctca tgatgagctt gctgctcagt
     1981 gtggacaata gctggatgaa cgtgctgttc ggtgggcgga acaacctggt gttcgcccgt
     2041 cacaaccgcg aatacggggc ctacgaactg cgtcagggat acaacaagcg gctgggcatc
     2101 gcactggtcg ccggcatcgg attcgtggtg ctcgcgttgg ggggcacgtg ggccagtgga
     2161 aagttcggcg gcgaagaaga ggtggaggtg aagaagaaga tcgtggatgt gaacatggaa
     2221 ctcttcgaag aggagaagga ggacgaacct gaggagatca aggagcccgt gctggtcgaa
     2281 caacccaaga tcgagaccgt gcagttcgtg gctgtggagg ccgcggacga aaaggtcgaa
     2341 gaacccccgc ccacccagag cgatgtggcc gagaccgtgg tgagcaccgt tacgcaagaa
     2401 ggggagaacg tggacgtggc gccaccgcca gcccccgtgg aggaagagac cttcgacctg
     2461 gccgccgttc aggagcagcc cgaattcccc ggtggcatgg aaaagatgta cgaattcatg
     2521 cagcggaccc agaagtaccc ggacatggag tacgaggcgg gcatccaagg caaggtcttc
     2581 gtgcagttcg tggtggacaa ggatggctcg atcgaggagg tgaaggtgct gcgggccgtg
     2641 agtgatggcc tggacaagga ggccctccgc ctggtcaaga gcatgcccaa atggagcccc
     2701 gggaaaatgg gcggcaaacc cgtgaagtgc cggttcaacc tgcccgtggt gttcaaactg
     2761 aaatagccgg gatcacatcc atgcggaagc cctgcccacg cggggcttct gcttttcagg
     2821 gaaaaggcgg agcgcttccc cgccattcgc cgttcgtgag cgaccgttcc tcgttcgctg
     2881 gacacggccc gcaatgaacc aggaagatcc ccggttgtac cgtgtacaga acgatccaat
     2941 aaaccccagg gaaccatgct caccatcgcc ctcttcctgc tctttgccct gctctgcgcc
     3001 gctctgagcg tcgatcgcac ttgggatagc gagaccatgt ccgagcgcaa tcaactggtc
     3061 tttgcgggcc gcaaccacga ctatggtgct tttgtacttc gtcgcgagta tgatcgtcga
     3121 ttcctcctcg ccttcgtggg ggccatcgcc ctctttggcg ccctcgttgc cgtgcccaag
     3181 gtgctcgggt ccatgggggt ctggtcggtg ccggccccgc cgcccgtgcc gtacatcccc
     3241 gatgaacggg tcatggagtg gttcaccacg ccggagaaca agcccaacga cccgcggcct
     3301 cctgcgccac gcacgaccac agcaccgccg accacatcgg acaacgataa cgtgaccgtg
     3361 gtaccgaagg acagtatgcc ccccgatgtg ccgaaggaca cactgcgcgt ggacccggag
     3421 cccgttactg gcaacccggg tccgatcggc ctggctggtg ccggacccgt gggtccaggc
     3481 accaacggcg ctggcggcga aaatggggat gggcctgctt tttccttgga caacccgggc
     3541 aaccccggca tcgtggatca ccagcccgag ttcccgggtg gtcaggaggc cctggcgcgt
     3601 ttcatccaga acaacctggt agtacgcgac gagggcatct cctccgcgcg gacgcaggtc
//
LOCUS       DAOQOC010000147         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12047_length_3659_cov_3.558546, whole genome shotgun sequence.
ACCESSION   DAOQOC010000147 DAOQOC010000000
VERSION     DAOQOC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12047_length_3659_cov_3.558546"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1340
                     /locus_tag="PL106_03415"
     CDS             210..1340
                     /locus_tag="PL106_03415"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNA32147.1"
                     /translation="MLNPLRTLAFADWRLGLDRPLLIAGPCSAESEEQVLGTARAIAD
                     GIPQVKVFRAGVWKPRTRPGGFEGAGDAALAWLKRVKAETGLLTMTEVAVPAHVEAAL
                     AVGIDMLWIGARTTPNPFSVQAIADALRGTDIPVFVKNPINPDTQLWVGAIERLADAG
                     ITRLAAVHRGFSWFERTPYRNSPMWEFPIRLKATFPQLELICDPSHIAGHPDRLAEIA
                     QQALDLNFSGLMIETHCDPARACSDAQQQIRPAVLRTLLQGLVERAPRPGPDALEGLH
                     ELRDLIDQLDEEIAQKLASRMDIAERIGEFKLAHNVAILQPERWDHIMRRQTALGGRI
                     GLDPGFVRAFMNAIHEESIRRQTAVWERVPESSEDPAMKTRT"
     gene            1473..>3659
                     /locus_tag="PL106_03420"
     CDS             1473..>3659
                     /locus_tag="PL106_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNA32148.1"
                     /translation="MRRLSSCTAAVLLGAVASAQAVFTEATANLSHASHGAACVAVAD
                     MDGDGLDDIVQLDMSEHVYILYQNADHSFTTFDYGQVENDSQWGWAMADLNNDGHKDI
                     CSGVGVTNFLNITSRGVYTLSDLNGPSIFTQAMSMADFNNDGRVDVFACNDVGPSNIW
                     ITNGSGVPEYDGNFMPWATQCTGTAGDMSGNYGSTVTDFDNDGDIDLHISHCRQGVNN
                     PDDCRRWDRLFVNDGNENYTDQAATYGLENHEQVWTTDFGDYDNDGDLDVFHTTHSTT
                     LQLFENDGTGHFTNVTEGSGLQYDGFFLQGLFRDLDNDTHLDIVTGSTHYYFKGNGDG
                     TFTRIDNVFPASKEMHSFAFGDLNGDGFEDVYATYGDGYVDGDENYPDKLWMNTPNGN
                     HFFNVDLEGTVSNRDAVGARVTITGPWGTMIREVHAGESYGITNTFTCHFGLGQYTTI
                     PTVTVSFPSGITQTFTDVHVDQNFTVVESECSSPNVAITSESGDLVWCPGDAGLTLNA
                     PEIEGYTATWNDGSTGTTFLVNGGGTYSVTLDNGDNCPVTAFANVLENPDQTPSITAD
                     GPLSFCETDGPVTLTSSSAIGNTWSTAQQTQSIVVNTSGSYTVTIDGACQQWTSAAVN
                     IDVADAPDAPTANGAIINYGATADLNATGSNVVWYDVATGGSAIASGNAFTTPALTAT
                     TSYWCADQNVSGGDIEYGGRTDQSNTGAMSNSAYHLLFDAFGPMVIN"
BASE COUNT          745 a         1240 c         1075 g          599 t
ORIGIN      
        1 ggacctgctg gaggagtgcc tgcacggcat cgccaaggcc catgccgatg gatggcttcg
       61 acccatggcg ctctccgagt tcccgtcgga acagctgccc gatgcgcaca cgctgctgga
      121 aagcggaagg agcatgggga aggtggttgt tcgttggtga tcgcagcaca tgcccggttc
      181 gggtgtcggg gtatctttgc aacgtgccgt tgctgaaccc gctccgcacc ctcgcattcg
      241 ccgattggcg gctcggtttg gaccgtccac tcctgatcgc cggcccgtgc agcgccgaat
      301 ccgaagaaca ggtactggga acggcccggg cgatcgctga tgggatcccg caggtgaagg
      361 tcttccgtgc aggcgtgtgg aagcctcgca cccggccggg cggcttcgag ggcgccggcg
      421 atgcggcgct cgcctggttg aagcgcgtga aggcagagac gggtctgctc accatgaccg
      481 aggtcgcggt gccggcgcat gtggaagcgg ccctggccgt gggcatcgat atgctgtgga
      541 tcggtgcacg caccaccccg aatcccttca gcgtacaagc catcgccgat gcgctgcgcg
      601 gcacggacat ccccgtgttc gtgaagaatc ccatcaaccc ggatactcaa ctctgggtag
      661 gagccatcga acgcctggcc gatgccggta tcacgcgcct ggccgccgtc caccgggggt
      721 tcagctggtt cgagcgcacc ccgtaccgca acagcccgat gtgggagttc cccatccggc
      781 tcaaggccac cttcccgcag ttggaactga tctgcgaccc cagtcacatc gccggtcatc
      841 cggatcgcct ggccgagatc gcccaacagg cgctggacct gaacttcagc gggctgatga
      901 tcgagacgca ttgcgacccc gcacgggcat gcagcgacgc tcaacaacag atccgccccg
      961 ccgtgctccg cactttgctc caaggtctgg tggagcgcgc tccccgaccc ggccccgacg
     1021 cccttgaggg attgcatgaa ctgcgggacc tgatcgacca attggacgag gagatcgccc
     1081 agaagctggc cagccggatg gacatcgcgg aacgcatcgg ggaattcaag ctcgcccaca
     1141 acgtcgccat cctgcagccc gagcggtggg accacatcat gcgcaggcag acggcattgg
     1201 gcgggcgcat cggtctggac cccggcttcg taagggcatt catgaacgcg atccatgagg
     1261 agagcatccg ccgacagacc gcggtttggg agcgggttcc ggagtcgtcc gaagacccgg
     1321 ccatgaagac ccggacctga ccggtccatc cgtaggaaac cgatgatcgt gcgctttcat
     1381 gcgccgaccg tgtatcccgt atcgggccac ctcctatttt gggcgatctc ttgcaaggaa
     1441 ccgttacgcc caacgaaccc cccaacaacc acatgcgacg tctttcctcg tgtacagcag
     1501 ccgtacttct cggtgcggtg gcctccgcgc aagcggtctt caccgaagcc accgccaacc
     1561 tctcccatgc ctcccatgga gccgcttgtg tggccgtggc cgacatggac ggtgacggac
     1621 ttgacgacat cgttcagctc gacatgagcg agcatgtcta catcctctac cagaacgccg
     1681 accacagctt caccacgttc gactacgggc aggtggagaa cgacagccag tggggctggg
     1741 ccatggccga cctgaacaac gatggccaca aggacatctg cagcggggtg ggcgtcacca
     1801 acttcctcaa catcacctcg cgcggggtgt acaccctgtc ggacctcaac ggcccgtcga
     1861 tcttcaccca ggccatgagc atggccgact tcaacaacga cggccgcgtg gacgtgttcg
     1921 cctgcaacga tgtgggacca agcaacatct ggatcaccaa cggcagcggt gttcccgagt
     1981 acgacggcaa tttcatgcct tgggccaccc aatgcaccgg aacagccggt gacatgagcg
     2041 gcaactacgg gagcaccgtc accgacttcg acaatgatgg cgacatcgac ctgcacatca
     2101 gccactgccg ccagggcgtg aacaaccccg acgactgccg ccgctgggac cgcctcttcg
     2161 tgaacgacgg caatgagaat tataccgatc aggcggctac gtacggcctg gagaaccacg
     2221 agcaggtgtg gaccaccgac ttcggtgatt acgacaacga cggggaccta gacgtgttcc
     2281 acaccaccca cagcaccacc ttgcaactct tcgagaacga cggcaccggc cacttcacca
     2341 acgtgaccga aggaagcgga ctgcagtacg acgggttctt cctccaaggc ctcttccgcg
     2401 accttgacaa cgatacccat ctcgatatcg tcaccggcag cacccactac tacttcaagg
     2461 gtaatggcga cggcaccttc acccgcatcg acaacgtgtt ccccgccagc aaggagatgc
     2521 acagcttcgc cttcggcgac ctgaacggtg acggcttcga ggacgtgtac gccacctacg
     2581 gcgacgggta cgtggacggc gatgagaact accccgacaa gttgtggatg aacacgccca
     2641 acggcaacca cttcttcaac gtggaccttg aaggcaccgt gagcaaccgc gacgccgtgg
     2701 gcgcccgtgt caccatcacc ggtccttggg gcactatgat ccgcgaggtg catgccggtg
     2761 agagctacgg catcaccaac acgttcacct gccacttcgg cctgggccag tacaccacca
     2821 tccccacggt caccgtcagc ttccccagcg gtatcacaca aacgttcacc gacgtgcatg
     2881 tggaccagaa cttcacggtg gtggaaagcg aatgctcctc gcccaacgtg gccattacca
     2941 gcgagagcgg cgacctggtg tggtgccccg gcgatgccgg cctcacgctg aacgccccgg
     3001 agatcgaggg ctataccgcc acctggaacg atggcagcac gggcaccacc ttcctggtga
     3061 acggcggcgg cacctacagc gtgaccctcg acaacggcga caactgccct gtgacggcgt
     3121 tcgccaacgt gctcgagaac cccgaccaga ccccgagcat cacggccgat ggcccgctca
     3181 gtttctgcga gacggacggc cccgtcaccc tcacctccag cagcgccatt ggcaacacct
     3241 ggagcacggc gcagcagacc cagagcatcg tggtgaacac cagtggcagc tataccgtta
     3301 ccatcgacgg cgcctgccag caatggacca gtgccgcggt caacattgat gtggccgatg
     3361 ccccggatgc gcctacggcc aacggtgcca tcatcaacta tggcgccacc gccgacctca
     3421 acgccaccgg ctccaatgtg gtctggtacg atgtggcaac cggcggatca gcgatcgcct
     3481 cgggcaatgc cttcaccact cccgcactca ctgccaccac cagctactgg tgcgccgatc
     3541 agaacgtcag cggtggcgat atcgaatacg ggggccgcac ggaccagtcg aacacgggtg
     3601 ccatgagcaa cagcgcgtac cacctgctct tcgatgcgtt tggtccgatg gtgatcaac
//
LOCUS       DAOQOC010000148         3648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12118_length_3648_cov_2.406067, whole genome shotgun sequence.
ACCESSION   DAOQOC010000148 DAOQOC010000000
VERSION     DAOQOC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12118_length_3648_cov_2.406067"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PL106_03425"
     CDS             <1..704
                     /locus_tag="PL106_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32149.1"
                     /translation="LSPVIVFEDVDVETGSRSYGYRVIVEDSCGVETLTSNPGESILM
                     AATAGTDGVNRLRWNGYQQWAGSVGGFNLYRSIGDGPFELIAQPGPLEWEAQDNVQAF
                     ITSNGRFCYYVEAIETGNPSGINATSTSNAACAVQQEAVWIPNAFDAGSSTVVNTVFK
                     PVVAFVDVEAYELIIYNRWGQQIWETTDANKGWDGRVNDHFVPQGTYAYYLGVRNGAD
                     KMYERRGMLTFLCCP"
     gene            complement(727..1482)
                     /locus_tag="PL106_03430"
     CDS             complement(727..1482)
                     /locus_tag="PL106_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32150.1"
                     /translation="MKTRHPLLLIGLVVTFASARAQGNALLDSAQAAYARGAYADALA
                     LYDSVYQEQTSAALLFNIGNCHMKLDDVPHAVLFYERAIRLEPGAEDIRANLDHARQQ
                     VVDRIHELPAFTLGSFWDRLRGGKDEDQWARRALWSCLALFAGATAFLFVRQGLVRRL
                     LLGGTALALICTVVATALGAYRVKEVNDRSEAIIMLPKVDVLGEPRQGATTVFVLHEG
                     TKISVLSDQNGWYEVKLASGSVGWAPPGSLERI"
     gene            complement(1479..3215)
                     /locus_tag="PL106_03435"
     CDS             complement(1479..3215)
                     /locus_tag="PL106_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNA32151.1"
                     /translation="MMRRILPFLWAMCVGIGMMAQEITFNAAVDRTSVATGEPIKLTL
                     TLSNAPMNSSIAPPDMGGLVVVQGPFDQNSFSMINGRTSSSVARTYYLQATKPGAYVI
                     GPATARVAGGTINTASIRITVVKGESGTASSAAAGQGQQKDPNLFCTISLSKNKAYVG
                     EQVLATYTLYSRYNRLDAGDYDLPKLDGFWAEEIDLGETSWAPAPQTVNGIAYRVAVL
                     KKQLLIPQRSGQLRIAPMKMEFRVNAGFFSNGTLVSITSNAETINVLDLPTKPEDFIG
                     AVGDLNMEVTVAATTVKANEPIDLGIKFNGRANLKLIDTPKLTFPSDFETYDPKVTDH
                     ITVNSGGMTGGRDYQFLLIPRHEGDYTVGPLSFSYFDPASGQYKRLTHEALSFHVEKG
                     DGSAVGISGPIRNDVQELHRDIRYIRTGDLSLEAKGAHLFGSGAWTAGMVAPALAFTG
                     LLLYRRRREKLLGDTSGMRRKGADRVAKKRLKDAATALQKNDREGFYTALGKALEGYF
                     ADRFDLGVAEVTPEKITEKLGHLEDGTLAREYTELMADSGMARFAPIEGKGRKQCYDE
                     ATSLIKRIEAAL"
     gene            complement(3217..>3648)
                     /locus_tag="PL106_03440"
     CDS             complement(3217..>3648)
                     /locus_tag="PL106_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32152.1"
                     /translation="PGEQARAFHNLGNSRMLQKQWQEAVNAYKQALRRAPGDEDTRYN
                     LAYAQKKLQEEQQKQKQQQQNKDQQKQQQDQQKQDQQQQDQQKQQDQQAKQQEQKQPG
                     QISKQDAERMLEAMQQQEKAAQKEVQKNIRVRPKEPIEKDW"
BASE COUNT          586 a         1133 c         1165 g          764 t
ORIGIN      
        1 ccctgtcgcc cgtgatcgtc tttgaggatg tggatgtgga gaccggttcg cggagctatg
       61 gttaccgggt gatcgtggag gacagctgcg gtgtggagac gctcaccagc aacccgggcg
      121 agagcatcct gatggcggca actgccggta ccgacggcgt gaatcgcctg cgctggaacg
      181 gctaccagca atgggcgggg tcggtgggcg gattcaacct gtaccgctcc atcggggacg
      241 gaccgttcga actcatcgcc caacccggtc cgctggaatg ggaggcccag gacaacgtgc
      301 aggccttcat caccagcaat ggccgcttct gctactacgt ggaggccatc gaaacgggca
      361 atccgtcggg catcaacgcc accagcacca gcaacgccgc ctgcgccgtt cagcaggagg
      421 ccgtctggat ccccaatgcg ttcgatgcgg gcagttccac ggtggtgaac accgtgttca
      481 aacccgtggt ggccttcgtg gacgtggagg cgtatgaact catcatctac aaccgctggg
      541 gtcagcagat ctgggagacc accgacgcga acaagggctg ggatggccgg gtgaacgacc
      601 atttcgtgcc ccagggcacc tatgcctatt acctgggcgt gcggaacgga gcggataaaa
      661 tgtacgaaag acgggggatg ttgaccttcc tgtgctgccc gtgaggccgg taggaggaag
      721 ttggcctcag atccgctcca gcgaccccgg tggtgcccag cccacgctgc cactggcgag
      781 tttcacctcg taccagccgt tctggtcgct caataccgag atcttggtgc cctcgtgcag
      841 cacgaagacg gtggtggcgc cttggcgggg ctcgcccagc acatcgacct tgggcagcat
      901 gatgatggcc tcggaacggt cgttcacctc cttcacccga taggccccga gcgccgtagc
      961 gaccacggtg cagatcaacg ccagcgccgt gcccccgagc agcagccggc gtaccaggcc
     1021 ctgtcgcacg aacaggaagg ccgtggcccc ggcgaaaagc gccaggcagg accacagcgc
     1081 ccgtcgtgcc cattggtcct cgtccttgcc tccacgcaac cggtcccaga acgaacccag
     1141 cgtgaacgcg ggcaactcat gtatccgatc caccacttgc tggcgcgcgt ggtccaggtt
     1201 cgcccggatg tcctccgcac cgggttcaag acggatggcg cgctcataga agaggacagc
     1261 gtgcggcaca tcgtccagct tcatgtggca gttgccgatg ttgaagagca acgcggccga
     1321 agtctgctcc tggtacaccg agtcgtacag ggcgagggca tcggcgtatg cgccacgggc
     1381 ataggccgcc tgtgcgctgt ccagcaaggc gttcccctgg gcgcgagcgg aggcgaacgt
     1441 gaccacgagg ccgatcagca gaagggggtg gcgggtcttc atagtgcggc ctctatgcgt
     1501 ttgatgagtg aggtggcctc gtcgtagcac tgcttgcgac ctttgccctc gatgggggcg
     1561 aagcgcgcca tgccgctgtc cgccatcagt tccgtgtatt cccgtgcgag ggtcccgtcc
     1621 tccaaatgcc ccaatttctc ggtgattttt tccggcgtca cctccgccac gcccaggtcg
     1681 aaccggtcgg cgaagtagcc ttcgagcgct ttgccgagcg cggtataaaa accttcgcgg
     1741 tcgttcttct gcagggccgt ggcggcatcc ttcagtcgct tcttcgccac gcgatccgcg
     1801 cctttgcgcc tcatcccgct cgtgtcgccc agtagtttct cgcgacgccg acggtacagc
     1861 agcaggcctg tgaaagcgag tgccggagcc accatgccgg ccgtccaggc acctgatccg
     1921 aacaggtggg cccccttggc ctccagcgac agatcgcccg tgcggatgta ccggatgtcc
     1981 cgatgcagtt cctgcacatc attgcggatc ggaccgctga tgcccaccgc gctgccgtcg
     2041 cctttttcca catggaagga cagggcctcg tgcgtgagcc gcttgtactg accggatgcg
     2101 ggatcgaagt aactgaacga caatggcccc accgtgtagt cgccctcgtg ccgcgggatc
     2161 aggaggaact ggtagtcccg gccaccggtc atgccaccgc tgttcacggt gatgtggtcg
     2221 gtcaccttcg ggtcgtacgt ctcgaagtcg ctggggaagg tgagcttggg cgtgtcgatg
     2281 agcttgaggt tggccctgcc attgaacttg atgcccagat cgatcggctc gttggccttc
     2341 accgtggtgg cggcaaccgt cacttccata ttcaggtcac ctacggcacc gatgaagtcc
     2401 tcgggtttgg tcggcaggtc cagtacgttg atcgtttcgg cattgctggt gatcgaaaca
     2461 agggtgccgt tgctgaagaa acccgcgttc acacggaact ccatcttcat gggagcgatg
     2521 cgcaactggc cggaccgttg ggggatgagc aactgcttct tcaggacagc gacccgatag
     2581 gcgatgccgt tcaccgtttg cggggcaggt gcccagctgg tctcgcccag gtcgatctcc
     2641 tcggcccaga aaccatcgag tttgggcagg tcatagtcgc ccgcatccag gcggttgtag
     2701 cgcgagtaca aggtgtaggt ggccagcacc tgctcgccca cgtacgcctt gttcttgctc
     2761 aggctgatgg tgcagaacag gttgggatct ttttgctgac cctggccggc ggcggcgctg
     2821 gaggccgtac cgctttcgcc cttgaccacc gtgatccgga tggacgcggt gttgatggtg
     2881 ccgccggcca cgcgcgctgt ggccgggccg atgacgtatg cgccgggctt ggtggcctgc
     2941 aggtaatagg tgcgcgccac cgagctgctc gtacgcccat tgatcatgct gaagctgttc
     3001 tggtcgaacg gcccttgcac caccaccagc ccgcccatgt ccggcggtgc tatggaggag
     3061 ttcatcggtg cgttgctgag cgtgagggtg agtttgatgg gctcgcccgt ggccacgctc
     3121 gtccgatcca ctgcggcgtt gaacgtgatc tcctgggcca tcatgccgat gcccacgcac
     3181 atggcccaca agaaggggag gatccgacgc atcatgtcac cagtcctttt cgatgggttc
     3241 cttggggcgc acgcggatgt tcttctgcac ctctttctgc gcggccttct cctgttgttg
     3301 catggcctcc agcatgcgct cagcgtcctg cttgctgatc tggccgggct gcttttgctc
     3361 ctgctgcttg gcctgctggt cctgttgctt ctgctggtcc tgctgttgct gatcctgctt
     3421 ctgctggtcc tgctgttgct tctgctggtc cttgttctgt tgctgttgct tctgcttctg
     3481 ttgctcctcc tgcagtttct tttgggcgta ggcgaggttg tagcgcgtgt cctcgtcgcc
     3541 gggtgcgcgg cgaagggcct gcttgtacgc gttgacggcc tcttgccact gcttctgcag
     3601 catgcggctg ttgcccaaat tgtggaaggc cctggcctgt tctccggg
//
LOCUS       DAOQOC010000149         3639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12177_length_3639_cov_4.102400, whole genome shotgun sequence.
ACCESSION   DAOQOC010000149 DAOQOC010000000
VERSION     DAOQOC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12177_length_3639_cov_4.102400"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..588)
                     /locus_tag="PL106_03445"
     CDS             complement(58..588)
                     /locus_tag="PL106_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32153.1"
                     /translation="MIPFHTMDMDSFHRLLGETAAARMVRARAQRLDQLRDTGTGLEP
                     VRVLDHVAYINDSGSTHLDATLRTMADLDRPVLWIAGALDASIGQPHVSEFLRERIVA
                     LVLHGKRAGQGIEALKPFTEHVYTTSELRTAVFVARELARAGEAVLFSPACPASDTHT
                     DAASRELEFLHAVSDL"
     gene            complement(642..2000)
                     /gene="murD"
                     /locus_tag="PL106_03450"
     CDS             complement(642..2000)
                     /gene="murD"
                     /locus_tag="PL106_03450"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNA32154.1"
                     /translation="MTRGQALSGTLVVLGAQESGTGAAVLAKKRGLPVFVSDAGRIKE
                     NYRNVLIEHDIEFEEGGHSAARVLSAGEIVKSPGIPDSASLVRAATEKNIPVISEIEF
                     ASRFTKGTIVGITGSNGKTTTTLLTGHILKKAGLDVAVGGNVGTSFASLVAERDRPLY
                     VLELSSFQLDGIREFRPHIAILTNITPDHLDRYAYRMSNYVASKFRITMNQRDNDHFI
                     HCADDPGTITGLGLHNVIARRWPFSLNNAQPLGAHLLDKTIHINTDNQTPFSMSILEL
                     ALQGRHNLYNSMAAGIAARVLEVRNETIRESLSDFQNVEHRLERVATVNGVEFINDSK
                     ATNVNSTWFALESMEKPVIWIVGGTDKGNDYSTLQDLVKSKVKAIVCLGKDNTPIHKA
                     FAHLDKPMVDTESAAAAVQQGYDLAEAGDIVLLSPACASFDLFENYEDRGRKFKAAVR
                     TL"
     gene            complement(1997..3247)
                     /gene="mraY"
                     /locus_tag="PL106_03455"
     CDS             complement(1997..3247)
                     /gene="mraY"
                     /locus_tag="PL106_03455"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNA32155.1"
                     /translation="MLYHLFKAIDFSIPGSGAFNYITFRAGMALFVSLVITLWFGKGI
                     INALRRMQVGETVRDLGLEGQMQKQGTPTMGGLMIIAGIVIPTLLFARLNNVYILLLL
                     ASTLWLGAIGFIDDYIKVFKKDKGGLAGTSKIIGQIGIGVVVGLTVYFHPSIRTKVEI
                     PEVLAQQFDESEITRYQDKQGGNHFLLDRKSPNTTIPFIKDNEFNYSSILKPFGKGVR
                     DNWTWLVYVLAVIFIITAVSNGANITDGIDGLAAGTSAVIVLTLGILTYIGGNIMFSQ
                     YLDVMYIPGIGELVVFIGAMLGACIGFLWYNTYPAQVFMGDTGSLMLGGTIATLAILV
                     RKELMIPVLCGIFLVENLSVVAQVSWFKYTKRKYGEGRRILKMSPLHHHYQKLGYHES
                     KIVARFWIVGVMLAVLTLVTLKLR"
     gene            complement(3250..>3639)
                     /locus_tag="PL106_03460"
     CDS             complement(3250..>3639)
                     /locus_tag="PL106_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNA32156.1"
                     /translation="NDTCRDDQRVICVVGCGGDRDRAKRPVMARIAAAMSHRIVLTSD
                     NPRSEDPMAILDEMRAGLDAADLPRVLVNADRREAIRTAVGLARPGDVVLVAGKGHET
                     YQEINGVKQPFNDVEVLKETLELLTRP"
BASE COUNT          608 a         1076 c         1214 g          741 t
ORIGIN      
        1 atggggtcgc ggcaaggccc gggcggaggg tatcgcggcc gggccgtatc gttcgcatca
       61 caggtcggat accgcgtgca ggaattccag ttcccgggat gcggcgtccg tatgtgtatc
      121 gctggcagga caggcggggg agaagagcac ggcctcgccg gcacgggcca gttcgcgtgc
      181 cacgaacacg gctgtgcgca gttcactggt cgtgtacaca tgctcggtga acggcttcaa
      241 ggcctcgatg ccctgacccg cacgcttgcc atggagtaca agggccacga tgcgttcgcg
      301 taggaattcc gaaacatgcg gctggccgat cgatgcgtcc aacgcgcccg cgatccagag
      361 cacgggtctg tcaaggtcgg ccatggtgcg caaagtggca tccaagtgcg tactgccgct
      421 gtcgttgatg taggccacat ggtcgagcac gcgcacgggt tccaggccgg tcccggtgtc
      481 acggagctga tcgagccgct gcgcgcgtgc ccgtaccatg cgggcggcag cggtctcgcc
      541 gagcagtctg tggaagctgt ccatgtccat cgtatggaac gggatcatcc ccgtcaggta
      601 ttgattgatg tttctccccg gtgttgcggc ccgttccggg ttcacagtgt gcgtaccgcg
      661 gccttgaatt tccggccccg gtcctcgtag ttctcgaaga ggtcgaatga ggcgcaggcc
      721 ggggacagga gcacgatatc gccggcctcg gccagatcat agccctgttg cacggcggcc
      781 gcggcgcttt cggtgtccac catgggcttg tccaggtgtg cgaaggcctt gtggatgggc
      841 gtgttatcct ttccgaggca cacgatggcc ttcaccttgc tcttcaccag gtcctgcagt
      901 gtggagtagt cgttgccctt gtcggtacca cccacgatcc agatcaccgg cttctccatg
      961 ctctcgagcg cgaaccaggt gctgttcaca ttggtggcct tgctgtcgtt gatgaactcc
     1021 acaccgttca cggtggccac gcgttccaga cggtgttcca cgttctggaa atcactcagg
     1081 ctctcgcgga tggtctcgtt gcgcacttcc agcacacgcg cggcgatacc ggcggccatg
     1141 ctgttgtaca agttgtggcg gccctggagt gcgagttcga ggatgctcat gctgaacggt
     1201 gtttggttgt cggtgttgat gtggatcgtc ttgtcgagca gatgcgcgcc gagcggctgc
     1261 gcgttgttga gggagaaggg ccagcgtcgc gcgatcacgt tgtgcagccc gagcccggtg
     1321 atggtcccgg ggtcgtccgc gcagtggatg aagtggtcgt tgtcccgctg gttcatcgtg
     1381 atgcggaact tgctggccac gtagttgctc atgcggtacg cgtagcggtc caggtggtcc
     1441 ggggtgatgt tggtgaggat ggcgatgtgc ggccggaact cgcgtatccc gtcgagctgg
     1501 aagctggaga gctcgaggac gtacaggggc cggtcgcgtt cggccaccag cgaagcgaac
     1561 gaggtgccca cgttgccgcc aacggccacg tccagaccgg cctttttcag gatgtgcccg
     1621 gtgagcaggg tggtggtggt cttgccgttg ctgccggtga tgccgacgat ggtgcctttc
     1681 gtgaagcgcg acgcgaactc gatctcgctg atcacgggga tattcttctc cgtggcggcg
     1741 cgcacgagcg aggcgctgtc ggggatgccg ggactcttca cgatctcgcc ggccgagagc
     1801 acacgtgccg cgctgtgccc gccctcctcg aattcgatgt cgtgctcgat cagtacgttc
     1861 cggtaatttt ctttgatgcg cccggcatcg ctcacgaaca cgggcagccc ccgttttttc
     1921 gcgagcactg ccgcgccggt gccgctctcc tgcgcaccga gcaccacgag ggtgccgctc
     1981 agggcctgtc ctcgggtcac cggagtttga gggttacgag ggtgaggacg gcgagcatca
     2041 caccgacgat ccagaaacgg gccacgatct tgctctcgtg gtaaccgagt ttctggtagt
     2101 gatggtgaag ggggctcatc ttgaggatcc gccgcccctc cccgtatttc cttttcgtgt
     2161 acttgaacca ggagacctgc gccaccacgc tgaggttctc cacgaggaag atgccgcaca
     2221 ggacggggat catgagctcc ttgcgcacga ggatcgccag cgtggcgatg gtgcctccga
     2281 gcatgaggct gcccgtgtcg cccatgaaga cctgggcggg gtaagtattg taccagagga
     2341 agccgatgca tgcgcccagc atcgcaccga tgaagaccac cagctcgccg atgccgggta
     2401 tgtacatcac gtccaggtac tggctgaaca tgatgttgcc gccgatgtag gtcaggatgc
     2461 cgagcgtgag cacgatgacc gcactggtgc cggcggccag tccgtcgatc ccgtccgtga
     2521 tgttggcgcc gttgctcacg gcggtgatga tgaagatcac ggcgagcacg tagaccagcc
     2581 aggtccagtt gtcgcggaca cccttcccga acggtttcag gatggatgag tagttgaact
     2641 cattgtcctt gatgaacggt atggtcgtat tcggtgactt ccgatcgagc aggaagtggt
     2701 tcccgccctg tttgtcctgg tagcgggtga tctcactctc atcgaattgc tgggccagca
     2761 cctcggggat ctccaccttg gtgcggatgg agggatggaa gtagaccgtg aggccgacga
     2821 ccaccccgat gccgatctgg ccgatgatct tgctggtgcc ggccagcccg cccttgtcct
     2881 tcttgaatac cttgatgtag tcgtcgatga aaccgatggc gccgagccag agcgtactgg
     2941 cgagcagcag gaggatgtac acgttgttca ggcgggcgaa aagcagcgtg gggatcacga
     3001 tacccgcgat gatcatcagg ccccccatgg tcggggtgcc ctgcttctgc atctggccct
     3061 cgaggcccag gtcccgcacc gtttcgccca cctgcatgcg ccggagcgca ttgatgatgc
     3121 ccttgccgaa ccagagcgtg atcaccaggg acacgaacag cgccatgccc gcacggaacg
     3181 tgatgtagtt gaacgcaccg ctgccgggga tagagaagtc gatggccttg aacaggtggt
     3241 agagcatggt tacggacggg tcaggagttc gagcgtctcc ttcagcacct cgacatcgtt
     3301 gaagggttgt ttcaccccgt tgatctcctg gtatgtttca tggcccttgc cggcgaccag
     3361 gaccacatca ccgggccggg ccaggcccac ggcggtgcgg atggcctcgc ggcgatcggc
     3421 gttcacgagc acccgcggca ggtcggcggc atcgagcccg gcgcgcatct cgtcgaggat
     3481 ggccatggga tcctcactgc gcgggttgtc actggtgagc acgatgcggt ggctcatggc
     3541 cgcggcgatg cgggccatca cggggcgttt tgcgcggtcg cggtcgccac cgcagcccac
     3601 cacgcagatc acgcgctgat catcgcggca ggtatcatt
//
LOCUS       DAOQOC010000150         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12205_length_3635_cov_3.226816, whole genome shotgun sequence.
ACCESSION   DAOQOC010000150 DAOQOC010000000
VERSION     DAOQOC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12205_length_3635_cov_3.226816"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PL106_03465"
     CDS             complement(<1..1067)
                     /locus_tag="PL106_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32157.1"
                     /translation="MAVTSVGDLFMGGYRSDSALVMRVDPLGHPVWSRTFRSTNAQRE
                     IVNQLTITPDGYLIGSGDSYGGSPAMYRSVLYFKFDLDGNAQWIRSSDDPRPAFSFAL
                     LPRTTEEYVLVDEMYDLTSPTFADPLSHGVNASDGTIIWAAPRLDYIPQNSYIDDAYG
                     ATIGSGGTYYTTGRIFLAGSAPGSMRSFISKFTSNGSHEWTRYHLIPGSSDARIYGCD
                     MITSTDSLLVCYTGDITQYSSTFRVGLIRTDTSGITAWAKDYDITEFGSEIAVKVLRM
                     PYGYAITGRSLDTDPDLFVLAVSLQGEVLWAKRYGSPFTSDKLLHSYLKNSCAIGSDV
                     VFTGEREEGGDSDIALYRIDST"
     gene            1266..2753
                     /locus_tag="PL106_03470"
     CDS             1266..2753
                     /locus_tag="PL106_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNA32158.1"
                     /translation="MGTLARQSSWIAVLTVAGLVLGFVNMSLLYPRYLPAAEFGLTRL
                     VVSIAIVAAQFAQLGAEGTVIRYYPYFRGRSSRNGGLFRWALLVGSAGAVIAILVLIL
                     FHGRLSTWFNDRSGLYGTHGLVVIPLLVAEVYYLLFRGASRAVHRSVAPMFAREFLLR
                     ALQTGLIAAHVLWDLPFPLFLGLFACTFVISTVTLIVDLWRAGDFTLTLDPVRPSRRL
                     IRSMARYAAVTLGVGVAGVAAGNVDQVMLAAMLPDGLEYVAYYAVAMMLASVVMVPSR
                     AMTMPALPLIAEAWRRRDLGTIRSLHARSTTVLLTLGFYTALCICVNTDVIYSWMKPG
                     YSTGAKALLILCVVNLVNLAGGLGGSIIGTSRRYAFDASSGLLYLGLNILLDFFFIRL
                     WGMEGVAWSSLVAMVVVVAWRTVFLWHRFKLWPYEASALIKLTGAVLVSLLAWWVPST
                     GTPLYDGLLRCVVITVPYWVVVFWTGACPELIEQAKKIARRVSLR"
     gene            complement(2656..>3635)
                     /locus_tag="PL106_03475"
     CDS             complement(2656..>3635)
                     /locus_tag="PL106_03475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA32159.1"
                     /translation="IRNRSWTRDCICHFSGYHAVLPTLLCRRTFIILAGSDCASIPSI
                     GYGAHARRLLGRATGLAASGATRLLPVHERLMRRHQTYSDAVPSEQGLAAFARVDHVP
                     WTVVPYGFDASFWCPDSTVDRDPGLFVCVPGPVAPENRLHALKGIDLVLKIAERIPQA
                     RFLVVGLADTAAYKSASPNVEFIGRLPPEQLRDLYRSSSFHLQLSLSEGMPNALCEAM
                     LCGCIPLVSAVSSMPDIVRGAGRILERKDVDQAVRICIELLAMTAEQRAIRSHGSRER
                     IRAEFPMNRRISTLTCLLNEGTPGGRSSWPARSTQDRPPSRTPPPSTGR"
BASE COUNT          689 a         1003 c         1169 g          774 t
ORIGIN      
        1 gtagagtcga tgcggtacag tgcgatatcg gagtcccctc cttcttctcg ctctccggtg
       61 aagaccacgt cagagccgat cgcacatgaa ttcttcaaat aggagtgaag cagcttgtcg
      121 gaagtgaatg ggcttccgta gcgcttcgcc caaaggacct ccccttggag ggacacggcc
      181 agcacgaaga ggtcggggtc ggtatcgagt gatcgaccgg tgatggcgta gccatagggc
      241 atgcgaagca ccttcactgc gatctcggac ccgaattcgg tgatgtcgta atccttggcc
      301 cacgccgtga tacctgatgt gtccgtacgg atgagcccga cccggaaggt ggaggagtac
      361 tgtgtgatat ccccagtata gcagaccagc agactatcgg tcgaggtgat catatcacac
      421 ccgtagatcc gcgcgtcgct cgatccggga atgagatggt aacgtgtcca ttcgtgtgat
      481 ccattgctcg tgaacttgct gatgaaggat cgcatactgc cgggcgcgga gccggccagg
      541 aagatccgtc ccgtggtgta gtacgttcca ccggagccga tggtggcgcc ataggcgtca
      601 tcgatgtacg agttctgagg gatataatct agtcgaggtg cggcccagat gatggtgccg
      661 tcggaagcat tcaccccatg ggacagcggg tcggcgaatg tgggggaggt gagatcgtac
      721 atctcatcga cgagaacata ctcttcggtt gttcttggca acagggcaaa tgaaaaagcg
      781 ggcctgggat catcggagga tctgatccat tgagcgtttc catcgagatc gaatttgaaa
      841 tacagcaccg accggtacat agccgggctt cctccgtagc tgtcacccga tccgatgagg
      901 tacccatcgg gtgtgatggt cagttggttc acgatctcgc gttgcgcgtt ggtggatcgg
      961 aaggtgcgcg accatacagg atggcccaac gggtccacgc gcatgaccaa tgcactatcg
     1021 ctgcggtagc cacccatgaa aagatcaccg accgaggtta cggccatggc cgaacctccc
     1081 tcgtcgatcg ttgcggaccg gaccgcgcca tgaacgtctg ggcccgtacg ggcagagtga
     1141 ggcaaggacg caggagcaac agccagggca ggaagcagtt cggtcgaccc atcagacggg
     1201 ccaaggtacg taagtggtgc cgcctgctgc cgcagggcgg tgtggccgaa ctaccttccg
     1261 cgccgatggg gacattggcc agacaaagtt cgtggatcgc tgtgctcacg gtggccggac
     1321 tggtcctggg tttcgtgaac atgtccttgc tgtacccgcg atacctcccg gcggctgagt
     1381 tcggattgac gcgacttgtg gtctcgatcg ccattgttgc cgctcagttc gcgcaactgg
     1441 gggcggaagg gacagtgatc cggtattatc cctatttccg tggaagatca agtcggaacg
     1501 gcgggctctt ccggtgggct cttctggtcg gctcggccgg agcggtgata gccatcctgg
     1561 ttctgatcct gtttcacggt cgattgtcca cctggttcaa tgaccggagc gggttgtatg
     1621 gaacgcatgg tctggtggtc attccgttat tggtcgctga ggtctactac ctgctcttca
     1681 gaggtgccag tagagcggtg catcggagcg ttgcgccgat gttcgcaagg gaattcctcc
     1741 tgcgtgcgct acagacgggg ttgatcgcgg cgcatgtgct ttgggacctg cccttcccgc
     1801 tttttctcgg tctcttcgca tgcaccttcg tgatcagtac ggttacgctg atcgtcgatc
     1861 tgtggcgcgc cggggacttc acgttgaccc tggacccggt acggccatct cggcgactca
     1921 tccggagcat ggcccggtac gcagcggtca ccttgggtgt cggggtcgcc ggggtcgctg
     1981 ccggcaatgt ggatcaggtg atgctggcgg ccatgctccc ggacggactg gaatatgtgg
     2041 cctattacgc cgtggccatg atgctggcca gtgtggtgat ggtgccttca cgagcgatga
     2101 ccatgcctgc actgccgctg atcgccgagg cgtggagacg gcgtgatctg ggaaccatcc
     2161 gatcgctgca cgcccgttcg accaccgtgc tcctcaccct ggggttctac acggcgctgt
     2221 gcatctgtgt gaatactgac gtgatctatt cttggatgaa gcctggatac tccaccggag
     2281 cgaaggccct gttgatcctg tgcgttgtga atctcgtcaa ccttgccggg ggcttgggtg
     2341 gaagcatcat cgggacatcg cgcagatacg ccttcgacgc cagctcgggc cttttgtacc
     2401 tgggcctcaa catcctgctg gatttcttct tcattcgtct ctggggcatg gaaggcgtgg
     2461 cttggagttc gctcgtggcc atggtggtgg tggtggcctg gcgaacggta ttcctgtggc
     2521 atcgcttcaa gctatggccg tacgaggcgt cagcattgat caagttgacc ggagccgtac
     2581 tggtatcgct gctcgcgtgg tgggttccaa gtacgggcac ccccttgtac gatgggctgc
     2641 tccgatgcgt ggtgatcacc gtcccgtact gggtggtggt gttctggacg ggggcctgtc
     2701 ctgagttgat cgagcaggcc aagaagatcg cccgccgggt gtcccttcgt tgagcagaca
     2761 tgtcagcgtg gagatccgtc ggttcatagg gaattctgca cggatccgct cgcgtgatcc
     2821 atgggatcgg atcgcgcgtt gctcagcggt catcgcgaga agttcaatac agatccgcac
     2881 cgcttgatcc acgtccttgc gttccagtat gcgcccggca ccacggacga tatcgggcat
     2941 ggatgatacg gcactcacca ggggtatgca gccgcagagc atggcttcgc agagggcgtt
     3001 gggcatgcct tcggacaacg aaagctggag gtggaaactg cttgatcggt acaaatcccg
     3061 aagctgctcc ggaggaagac gtccgatgaa ctccacattg ggtgaggcac tcttgtatgc
     3121 cgcggtgtcg gccaggccca ccaccaggaa tcgggcctgc ggtatgcgtt ccgctatctt
     3181 cagcaccagg tcgatcccct tcaaggcgtg taagcggttc tccggtgcaa cagggccggg
     3241 aacacacacg aacagcccgg ggtcgcggtc aacggtgctg tccgggcacc agaatgaggc
     3301 atcgaagcca tagggaacga ccgtccaggg aacgtgatcc acgcgtgcga acgcagcaag
     3361 accttgttcg ctgggaactg cgtccgaata ggtctgatgg cgccgcatca ggcgttcatg
     3421 cacggggagg aggcgagtag ccccggaagc ggccaaccct gtggccctgc caagcaagcg
     3481 gcgcgcatga gcaccataac cgatggaagg aatgctcgca caatcgcttc cggccaggat
     3541 gatgaaggtc ctgcgacaga gcaacgtggg cagtaccgcg tggtatccac tgaaatggca
     3601 gatgcagtcc cgtgtccatg aacgattccg gatca
//
LOCUS       DAOQOC010000151         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12267_length_3623_cov_3.380325, whole genome shotgun sequence.
ACCESSION   DAOQOC010000151 DAOQOC010000000
VERSION     DAOQOC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12267_length_3623_cov_3.380325"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1759)
                     /locus_tag="PL106_03480"
     CDS             complement(149..1759)
                     /locus_tag="PL106_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32160.1"
                     /translation="MRTLAPLTLIASILFATWSSNTNAQQQFLQYDRLFGGPDKETCG
                     QMIPGVDGGYLIVGSTGSYGTGASDQNPNGFVVKVDANGEPEWRIALGTFGHDFYSGA
                     TVTDDGNYLIIGTRVFSGVQDTDMTLTKVSPAGDVLSIKEIGTTGRSEQGYRILAVGN
                     GEYVVIASRYLTGGGVDGNFLVFRTDDAGNITWTGEYGCGQQMDPKAITFAADSTLLI
                     AGARYDLPVSHAVLIKVALDGTLVWGSTDQQVENSTSNIALVATEEGGCYTTGVGGTD
                     QLALARYDANGEVLWRQGYTTGSSNSNGRDMILMPDGSLCISAYIFFTDHPDQGAACI
                     LHTDTMGQVLDQVMTGPDIGFLSPRSLVLDGAEGLVFASNISSDDEPGDVEVVRTHLG
                     NNGWDEPCQPVQVGMQPMALPSLSPGPAITALPPPTLTVTTRDWIPLDANGSQTICTI
                     TGMTESLAPASARCWPNPAHDVLNYEWPGAIRIKDLSVYDAMGRRTLNTPPLAGSAIP
                     IPDLLRGIHTLLIIRENGERVVLPFVVE"
     gene            complement(1871..2632)
                     /locus_tag="PL106_03485"
     CDS             complement(1871..2632)
                     /locus_tag="PL106_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32161.1"
                     /translation="MRPLLLLLVATIGLRLSAQVQNWNPEPYRTDSALIFRDDLDYYL
                     NSYSEAEIKAMRKQVNIWRMNFDKVIRTTIDDLRAYSEGNNFIPRLHDFTLPTATDSS
                     VTYHLAARKGRIRAFMFASMTNPPARFQLPLWDRLLEKYTGQDVDLFTIYGKELHPKD
                     RPSFKAYPAPKSEYEKRRYARDLAAMTKIPVLVDGLDDAVLNAYGRVPNGVYVFDKDN
                     NLVFRGTWADSRKIEHILGTLLQWYADGKPKVGKE"
     gene            complement(2659..3291)
                     /locus_tag="PL106_03490"
     CDS             complement(2659..3291)
                     /locus_tag="PL106_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNA32162.1"
                     /translation="MIVALRRTDPYKPMRHFLIAAVLAPCFALAQHPTGSPATQAQGH
                     ATSKVKWLSIEEAQAAAKKDGKPLLIDFQTPWCGWCRKMENGTFNDEHTANYINANFH
                     PVSFNAEGPDSVTFNGKVFHNPQYNPAGPRHGTHQLAAYLAAVDGRLGYPTIAYVDSE
                     GNLIQPVQNYFTPEQIEPILAFFGTGAYKGQDWQTFHAAFKSTRPAPTGQ"
     gene            complement(3306..>3623)
                     /locus_tag="PL106_03495"
     CDS             complement(3306..>3623)
                     /locus_tag="PL106_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA32163.1"
                     /translation="TFAWFVITFGELCLSPIGLSLMTKLSPVRIQGLMMGMWFLASAY
                     GQYAAGLLGAGMSADNPDATNYDKLVLYTEGYGHLGLYALIAGAALIILSPLVRKLMR
                     EVH"
BASE COUNT          624 a         1021 c         1229 g          749 t
ORIGIN      
        1 ctatccggcc aggtgggctg gggtgcgcag ggggcgttcg cggcggtgcc catcagcgag
       61 agcatcctcg cggtgatcag tgcggtgatc ttccagcggg gaaggtggaa gttggtgaag
      121 gtgtgatcgc ttgatccgcg ctggaggatc actccaccac gaacggaagc acaacgcgtt
      181 ccccgttctc gcggatgatc agcagggtat ggatcccgcg caggaggtcg gggatcggga
      241 tggccgaacc tgcgagaggt ggtgtattca aggttcttcg ccccatggca tcgtacacgc
      301 tgagatcctt tatccgtatt gcaccgggcc actcgtagtt gaggacatcg tgtgcggggt
      361 tgggccagca gcgggcggag gctggtgcaa gactctcggt catgcccgtg atcgtgcaga
      421 tcgtttgcga tccattcgca tccaggggta tccaatcgcg ggtggtcacg gtaagggtgg
      481 gaggcgggag cgcggtgatg gcgggccctg ggctcagcga cggcaacgcc atcggttgca
      541 taccgacctg caccggttgg cagggttcgt cccagccgtt gttgcccagg tgtgtacgga
      601 ccacctcgac atcccctggt tcatcgtccg aggagatgtt ggatgcgaac acaagacctt
      661 ccgcaccgtc caacaccagc gatcgggggc tcaggaagcc gatatcgggg ccggtcatca
      721 cctggtccag tacctgcccc atggtatccg tgtgcaggat gcaggcagcg ccttggtccg
      781 gatggtccgt gaagaaaatg tacgctgata tgcagagcga accatccggc atcagtatca
      841 tatccctgcc gttggaattg ctggatccgg tggtatagcc ctggcgccag aggacctcac
      901 cattcgcatc gtatcgtgcg agcgcaagct ggtcggtgcc accgacaccg gtggtatagc
      961 acccaccttc ctcagtggcg accagtgcga tgttgctggt actgttctcg acttgttgat
     1021 cggtgctgcc ccacacgagg gtcccgtcca gggccacctt gatgagcacg gcgtgcgata
     1081 cgggcaagtc gtagcgtgct cccgcgatga ggagggtgct gtcggcggcg aaggtgatgg
     1141 ccttcggatc catctgttgt ccacagccgt attccccggt ccaggtgata ttgcccgcat
     1201 cgtcggtccg gaacaccagg aaattaccgt ccacgccgcc gcccgtcaaa tacctggatg
     1261 cgatgaccac gtactctcca ttgccaacgg ccaggatccg gtagccctgt tcagaacggc
     1321 cggtggtccc gatctccttg atgctgagaa cgtccccggc gggcgacact ttcgtcaggg
     1381 tcatgtcggt atcctgcacc ccgctgaaca cgcgtgtacc aatgatcagg tagttgccat
     1441 cgtcggtgac cgtggctcct gagtagaagt cgtgcccgaa ggtgcccagt gcgatccgcc
     1501 attccggttc gccgttcgca tccaccttca ccacaaaccc gttggggttc tggtccgacg
     1561 cgccggtgcc gtatgacccg gtgcttccca cgatcaggta tccgccatcg acccccggga
     1621 tcatctggcc acaggtctcc ttgtccggcc cgccgaacaa ccggtcgtac tggaggaatt
     1681 gctgttgggc attggtgttg ctgctccacg tagcgaacag gatcgaagcg atgagcgtga
     1741 gcggggcaag ggtgcgcatg gtcccaaagt tcgcatttgg cgggatattt cagttaggga
     1801 ccgcacaacc ggtggttccg gctgcacaag cggtcaggct ttatgcccgc ctctgcccaa
     1861 ccaccttgct ctattccttc cccaccttcg gcttcccgtc cgcgtaccac tggagcaagg
     1921 tgcccaggat gtgttcgatc ttccggctgt cggcccaggt accgcggaac accaggttgt
     1981 tgtccttgtc gaagacgtac acgccgttgg gcacgcggcc gtaggcgttg agcacggcat
     2041 catccaggcc gtccaccagc acggggatct tggtcatggc cgcgaggtca cgtgcatagc
     2101 gccgtttctc gtactcgctt ttcggtgcgg ggtaggcctt gaagctgggc cggtccttgg
     2161 gatggagttc cttgccgtag atggtgaaca ggtccacatc ctggccggta tatttttcca
     2221 gcagccggtc ccacagcggc aattggaacc gtgcgggcgg gttggtcatg ctggcgaaca
     2281 tgaaggccct gatccgtccc ttgcgggctg cgaggtggta ggtgacagag ctgtcggtgg
     2341 ccgtgggcag ggtgaagtcg tgcaggcgcg ggatgaagtt gttgccctcg ctgtacgccc
     2401 ggaggtcatc gatggtggtg cggatcacct tgtcgaaatt catgcgccag atgttcacct
     2461 gtttgcgcat ggccttgatc tcggcttcgc tgtagctgtt gaggtagtaa tccaggtcgt
     2521 cgcggaagat gagggcgctg tcggtgcggt agggttcggg gttccagttc tgcacctgtg
     2581 cggacaagcg caggccgatg gtggcaacaa gaagcaggag cagcgggcgc atcggatgcg
     2641 gcggtacgtg ttcgggactt actggccggt cggtgccggg cgggtgcttt tgaaggcggc
     2701 gtggaaggtc tgccagtcct ggcctttgta agcgccggtg ccgaagaagg cgaggatggg
     2761 ctcgatctgt tcgggtgtga aataattctg gaccggttgg atcaaattcc cttcgctgtc
     2821 cacataggcg atggtggggt aaccgaggcg accgtccacg gcggccaggt aggcggccag
     2881 ttggtgggtg ccgtgccgcg gcccggccgg gttgtactgc gggttgtgga agaccttgcc
     2941 gttaaaggtg acgctatcgg gcccttcggc gttgaaggag accgggtgga aattggcgtt
     3001 gatgtagttg gcggtatgct cgtcgttgaa ggtgccgttc tccatcttcc ggcaccagcc
     3061 gcaccacggg gtctggaagt cgatcagcag gggcttgccg tccttcttgg cggcggcctg
     3121 ggcctcctcg atggagagcc atttcacctt gctcgtggca tgcccttggg cttgggtagc
     3181 tggtgagcca gtcggatgct gcgcgagggc gaagcagggt gcgaggacgg cggcgatcag
     3241 gaagtggcgc atcggcttgt agggatcggt cctgcgaagg gcaacgatca tgccgccaag
     3301 gtacatcagt ggacctcgcg catcagtttg cgcaccaagg gcgagagaat gatgagcgca
     3361 gcgcccgcga tcagcgcgta aaggccgagg tgcccgtagc cttcggtgta cagcaccagc
     3421 ttgtcgtagt tggtagcgtc ggggttatcg gcgctcatgc ccgcgccgag cagaccggcg
     3481 gcgtattggc cgtatgcgct ggccaggaac cacatgccca tcatcaggcc ctggatgcgc
     3541 acgggggaga gcttggtcat cagagagagg ccgatgggcg acaggcagag ctccccgaag
     3601 gtgatcacga accaggcgaa ggt
//
LOCUS       DAOQOC010000152         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12366_length_3605_cov_3.582535, whole genome shotgun sequence.
ACCESSION   DAOQOC010000152 DAOQOC010000000
VERSION     DAOQOC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12366_length_3605_cov_3.582535"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PL106_03500"
     CDS             complement(<1..698)
                     /locus_tag="PL106_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32164.1"
                     /translation="MNHSATLILAVGLICACGQPANNMPSEQPRPPRAKKVPHQMVEH
                     GHTRNDEYFWMRLSEEQRHTPTPDAHTREVVAHLEAENAYADELLGPVKELREALFEE
                     MKARVKEDDLSVPYRENGFWYNHRFEKGKEYAVHMRGKAKPDGGCPEKLDDFINENTM
                     AEGHDYFDLGDFEISPDNRLCAYSVDTVSRRLYTIRFRDLLTGVELPDVITNTSGRDQ
                     PGDRGVLVAPAGSG"
     gene            complement(776..1501)
                     /locus_tag="PL106_03505"
     CDS             complement(776..1501)
                     /locus_tag="PL106_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HNA32165.1"
                     /translation="MLCTTRAVVLRTVKHTDGRVVLRAYTERLGLRSFLVRTGAGSAS
                     RSAPLQALARVELVVPDTGEPGLRTARDLRVDKPYVRAPYEPLRGILLLFAQEVFNKT
                     LREEAPDEGLFHFVGSALEDIDTGNDLAHQPLRLLIGLMAPLGFLPEPPGEGETRFDL
                     RDGMFFRGPSPHGFCMEPDQVSLFGALMARGVGPPRMKERQGSSTAGIRRRLLDDLLT
                     YFRLHVEAFGELRSVDVLRAVLE"
     gene            complement(1503..>3605)
                     /locus_tag="PL106_03510"
     CDS             complement(1503..>3605)
                     /locus_tag="PL106_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA32166.1"
                     /translation="NNRSALVVGYGNGNIDVVGSGSSVNLSDIMRSSLIGDKRIYSIT
                     CEGDLAYLGCGFGVVVLDLARYEVKDTWLIFPNAAQGRVNAITFHQDSIYAATQSGLY
                     SAWRNEPNLAAFTNWHQRTDAPQASGWFSEAVSFGGHLFVNWTEPPPSTAGTDTIYYW
                     DSGWHKLDGTFGRDVRSLSVSPDGGRLVVGATYETFQYDAALEWVFTGMTGAGVQIVL
                     NDAAGASNGGVWVACAQTGLGRCDNGGAGLMMAPNGPRTSSVVDLDIGSGLLMAATGS
                     VSGNWGNAYNHQGIFFFRDGQWRTVQESDDPLMMGANMYGGGAVDPVAVAVDPSDPGH
                     GYSGHWEEGILEWRDGVAQTIWNATNSSLGASGDPSEGIVYVGGLDHDEDGNLWVSNA
                     NTQNLVSVRKKDGSWKSFDPGGLLAGNHLVSRITALSNGQKWLVRPRTNGLLVFSDGG
                     TIDDTGDDQWKVLTTFENQGKLPTLDVFAVAEDQDGEVWVGTGKGIAVFYDPDAIFSG
                     ENYDCQQILIEQDGNVQILLETEVVSSIVVDGGNRKWLGTQTSGAFLVSPDGTEQIAH
                     FTKENSPLPSDNITDITIDGETGEVFFGTDQGIVSYRGEATTGERDASCASVFPNPVH
                     ATYQGPVAITGLIADSDVRITDMAGNLVYRTISFGGQAIWPGTDLSGQRVASGVYLVM
                     AASPDGLRHCNTKVLVTR"
BASE COUNT          643 a         1219 c         1048 g          695 t
ORIGIN      
        1 ttgcccgaac ctgccggcgc aacaagtaca ccacgatccc caggttgatc gcggccgctg
       61 gtgttggtga tgacgtccgg cagttccacg ccggttagca gatcgcggaa gcggatggtg
      121 tacaatcgtc ggctgaccgt atccacactg tacgcgcaca gcctgttgtc ggggctgatc
      181 tcgaagtccc ccaggtcgaa gtagtcgtgc ccttcggcca tggtgttctc gttgatgaag
      241 tcatccagct tctccgggca gcccccgtcc ggcttcgcct tcccgcgcat gtgtacggcg
      301 tattccttgc ccttctcgaa ccggtggttg taccagaacc cgttctcccg atacggcaca
      361 ctcagatcat cctccttcac tcgtgccttc atctcttcga aaagcgcttc gcgcagttcc
      421 ttgaccggac cgagcagttc gtcggcgtag gcgttctccg cttccagatg cgccacaacc
      481 tcccgtgtat gcgcatccgg tgtcggggta tgccgttgtt cttcgctcag ccgcatccag
      541 aagtactcgt cgttgcgggt gtggccgtgc tcgaccatct ggtgggggac cttcttggcc
      601 cgtggagggc ggggttgttc gctgggcatg ttgttcgccg gctgtccgca ggcacatatc
      661 aggccgacgg ccaggatgag cgtggcactg tggttcatgt ggtcggcccg tgggccatgg
      721 gtgatcgggc ggggaagtta tcccggccca ccatttcttc ccgacgggta tcggctcact
      781 cgagtaccgc acgaagaaca tccaccgaac gcagttcgcc gaaggcctcc acatgcagcc
      841 tgaagtacgt cagcaggtcg tccagcaatc ggcggcgtat tccagcggta ctcgacccct
      901 gccgttcctt cattcgaggg gggcccaccc cacgggccat cagcgcgcca aaaagcgata
      961 cctgatccgg ttccatgcag aagccatggg gcgacggacc ccggaaaaac atcccatcgc
     1021 ggaggtcgaa tcgcgtctcc ccctccccgg gcggttccgg caggaaaccc aggggggcca
     1081 tgagtccgat caggagccgt agcggttggt gcgccagatc gttaccggta tcaatatcct
     1141 ccagcgcgct ccccacgaag tggaacaagc cctcgtccgg agcttcctcc cggagggtct
     1201 tgttgaacac ttcctgggcg aagagcagca gtattccgcg caatggttcg tacggagcac
     1261 ggacatacgg cttgtccacc cgcaggtcac gagcggtacg cagaccaggc tccccggtat
     1321 ccggcaccac gagctccacc cgggccagcg cttgcagcgg tgcggaccgg gatgcgctcc
     1381 cggccccggt ccgcaccagg aacgaccgca ggcccaaccg ctcggtgtac gcccgcagca
     1441 caacacgtcc gtccgtgtgc ttcacggtac gcagcaccac cgcccgcgtg gtacagagca
     1501 tctcagcgcg ttaccaggac cttggtattg cagtgccgca ggccatcggg cgaggcggcc
     1561 atcaccagat acacgccgct ggccacccgt tgaccactga ggtcggtgcc cggccagatg
     1621 gcctggccgc caaaagagat cgtgcggtaa accaggttgc ccgccatgtc ggtgatccgc
     1681 acatcactgt cggcgatcag gccggtgatc gccacggggc cctggtacgt ggcatgcacc
     1741 ggattgggga acacgctggc gcaactggca tcacgctccc ccgtggtggc ctctcccctg
     1801 taactgacga tgccctggtc cgtgccgaag aacacctcgc cggtctcccc atcaatggtg
     1861 atgtcggtga tgttgtcgct gggcaacggg ctgttctcct tggtgaagtg agcgatctgt
     1921 tccgtgccat cggggctcac gaggaaagct ccactggtct gtgtgcccaa ccatttccgg
     1981 ttgccgccat ccaccacgat cgaactgaca acctcggtct ccagcaggat ctgcacgttg
     2041 ccgtcctgtt cgatcaggat ctgctggcag tcgtagttct caccggagaa tatggcatcg
     2101 ggatcgtaga ataccgctat gcccttgccg gtacccaccc acacctcccc atcctggtcc
     2161 tccgccacgg cgaacacgtc gagcgtgggc agtttgccct ggttctcaaa agtcgtgagc
     2221 actttccact gatcgtcgcc ggtatcgtca atggtaccac catcagagaa caccagcagt
     2281 ccgttcgtac gcgggcgcac gagccacttc tgtccgttcg acagtgcggt gatccgggag
     2341 acgaggtggt tgcccgccag caggccgccg gggtcgaacg acttccagga gccgtccttc
     2401 ttgcgcacac tgaccagatt ctgggtgttc gcgttcgaaa cccagaggtt gccgtcctcg
     2461 tcgtgatcga gtcccccgac atagacgatg ccttcggagg gatccccact ggcgccgagc
     2521 gaactgttgg tcgcgttcca gatggtctgg gcaacgccat cccgccactc gagtatacct
     2581 tcttcccaat gcccggaata accatgtccc ggatcggacg ggtccacggc cacggccacc
     2641 gggtccaccg caccgccccc atacatgttg gcacccatca tcagcggatc gtccgattcc
     2701 tgcaccgtgc gccactggcc gtcgcggaag aagaagatcc cctgatgatt gtatgcattc
     2761 ccccagttcc cactgacgga gccggtggcc gccatgagca gtccgctgcc gatgtccaga
     2821 tcgaccacac tgctggtgcg cggtccgttg ggtgccatca tcaggcccgc gcccccgtta
     2881 tcacatcgcc ccaggccggt ctgggcacaa gccacccaca cgcccccgtt tgaggccccc
     2941 gccgcatcgt tcaacacgat ctgtacgcca gcgcccgtca taccggtgaa cacccactcc
     3001 aacgcggcat cgtactggaa tgtctcgtac gtggccccca ctaccaatcg cccgccatcg
     3061 ggcgaaacgg acagggaccg cacgtcccgg ccaaaggtgc catccagctt gtgccaaccg
     3121 ctgtcccagt agtatatcgt gtccgttccg gccgtgctcg gtggcggttc cgtccagttc
     3181 acgaagagat ggccgccgaa cgaaacggcc tcggagaacc agccgctcgc ctgcggggca
     3241 tcggtacgtt ggtgccagtt ggtgaatgcg gccaggttcg gctcgttccg ccatgcactg
     3301 tagagccccg actgcgtggc ggcgtaaatg gaatcctgat ggaaggtgat cgcgttcacc
     3361 cggccctgtg ccgcgttggg gaatatgagc caggtatcct tcacctcgta gcgcgcaagg
     3421 tccaggacca ccacaccgaa cccgcagccg agatacgcca gatcaccctc gcaggtgatg
     3481 ctgtaaatac gcttgtcgcc gatcagggag ctgcgcatga tgtcgctcag attgacgctc
     3541 gagccgctgc ccaccacatc aatgttgccg ttgccgtaac ccacgaccag cgcactcctg
     3601 ttgtt
//
LOCUS       DAOQOC010000153         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12373_length_3603_cov_3.191375, whole genome shotgun sequence.
ACCESSION   DAOQOC010000153 DAOQOC010000000
VERSION     DAOQOC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12373_length_3603_cov_3.191375"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PL106_03515"
     CDS             <1..724
                     /locus_tag="PL106_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32167.1"
                     /translation="RVLIAATDGMRAYAARAYGPTTARWYTKPACVDVLRFAEQEVKR
                     PDKLRELGLEGRIVGLYTGKLGGIYLDQEVFDLFRAARDHWGENFRVLLLTPHPVSEL
                     EPMMARAGLDRSIFTVRSVPHEEVPGLMGLADFAVTPVKPVPSKRYCTPVKDGEYWAL
                     GLPVIITRDISDDSGIIREHGIGSVLESLDRPGYEKAVKEVAALLDGASRKELYQRIR
                     QIALRYRSFDRAERIYQEIYGE"
     gene            714..1742
                     /gene="galE"
                     /locus_tag="PL106_03520"
     CDS             714..1742
                     /gene="galE"
                     /locus_tag="PL106_03520"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNA32168.1"
                     /translation="MGNERILVTGGAGYIGSHTIVELLAVPGIEVVSVDNLSNSDPAV
                     FGRIARICGREVRNHVIDLCDARALDRMFANEAPFSGVIHFAAFKSVPESMADPRKYY
                     HNNINSLLNILETSVRNGVPKFIFSSSCSVYGNIERMPVSETTPLGRAESPYAHTKQV
                     GERIVGEYARAHPALAAISLRYFNPVGAHASGQLGEDPINPPSSLVPVITRTAIGKMA
                     RVTVFGTDYPTRDGSCIRDYVHVSDIANAHVKALDRAARNGTGVEVFNLGTGTGVSVL
                     EAIAAFEQVSGMRLPYELGQRRSGDVGAIWSDTTRSREALGWKAERDLLAMMGTAWAW
                     ERQRSEEK"
     gene            complement(2585..3469)
                     /locus_tag="PL106_03525"
     CDS             complement(2585..3469)
                     /locus_tag="PL106_03525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA32169.1"
                     /translation="MNAETTRTQRPVDPRLLVTNAAGPLISIILPARNAAPYLRECID
                     SVLAQTWSNWELLIVDNASTDGTHAIATSYSDPRISVFSESRRGVSPARNKGLAAMKG
                     GFFCFLDADDRLPQDALRLRHDLFLRYPEARFADGAMRGFDHRTGKTLWVRSPWFVGM
                     PFDALMALDGSCFAGNTWMVRRLPGYDYRLPEHMDHSEDCAFFLGIARQGRYVSTARE
                     VLHYRVGHPSATRDPERVHQGYIELYRNMRAQVPPPSPEQLTNAWRRLRRFMTRDLLL
                     HHRPWAALHARLMRPPRP"
BASE COUNT          689 a         1084 c         1175 g          655 t
ORIGIN      
        1 ccgcgtgctc atcgcggcca cggacggcat gcgcgcgtac gccgcccgcg cctacggacc
       61 gaccacggca cggtggtaca ccaaaccggc ctgtgtggat gtcctgcggt tcgccgaaca
      121 ggaggtcaaa cggccggaca agttgaggga actcgggctg gaagggcgca tcgttggcct
      181 ctacaccgga aagctcggcg gcatctacct ggatcaggag gtgttcgacc ttttccgtgc
      241 ggcacgcgac cactggggcg agaacttccg cgtcctgctg ctcacgcccc atcccgtgtc
      301 cgaactcgaa ccgatgatgg cacgcgcagg gctcgaccgg tccattttca cggtacggtc
      361 cgttccgcac gaggaggtcc ccggtctgat gggacttgcg gacttcgccg tcacaccggt
      421 gaaaccggta ccgtccaaaa ggtactgtac cccggtgaag gacggcgagt actgggcgct
      481 tggcctgccc gtgatcatca cccgggatat atccgacgac tccgggatca ttcgtgagca
      541 tggcatcggc agcgtgctgg agagcctcga ccgaccgggg tatgagaagg cggtcaagga
      601 ggtggcggca ctgttggacg gcgcttcgcg gaaggaactc taccaacgca tccgccagat
      661 cgcgctccgc taccggagtt tcgatcgggc cgaacgcatt tatcaggaga tctatgggga
      721 atgaacgcat tctggtgacc ggtggggccg ggtacatcgg ttcacacacc atcgttgaac
      781 tgctcgccgt gccgggcatc gaagtggtct cggttgacaa cctgtcgaac tcggacccgg
      841 ccgtcttcgg tcgcatcgcg cggatctgcg gccgggaggt ccgcaaccac gtgatcgacc
      901 tgtgcgatgc acgcgcgctc gaccggatgt tcgcgaatga agccccgttc agcggggtga
      961 tccacttcgc ggcgttcaag tccgtgccgg agtcgatggc ggatccgcgc aaatactacc
     1021 acaacaacat caattccttg ttgaacatcc tggagaccag cgtgcgcaac ggggtcccga
     1081 agttcatctt ctcctcatcg tgctcggtct atggcaacat cgaacgcatg cccgtgagcg
     1141 agacgacgcc tttgggccgg gcggagtcgc cctacgcgca taccaagcag gtgggggaac
     1201 ggatcgtggg ggagtacgcc cgtgcgcacc ctgcgttggc cgccatcagc ctgcggtatt
     1261 tcaatcccgt cggggcccac gcttcgggcc aactgggcga ggacccgatc aacccgccga
     1321 gcagcctggt gccggtgatc acgcgcacgg ccatcggcaa aatggcccgg gtcacggttt
     1381 tcggtacgga ctatcccaca cgcgatggtt cgtgcatacg cgactatgtc catgtttcgg
     1441 acattgccaa cgcgcacgtg aaagccctcg atcgtgcggc ccggaacggg accggggtcg
     1501 aggtgttcaa cctgggcacg ggcaccgggg tgagcgtgct cgaagccatc gcggccttcg
     1561 aacaggtcag cgggatgcgg ctcccgtacg aactgggtca acggcgatcg ggcgacgtgg
     1621 gcgcgatctg gtccgacacc acgcgctccc gcgaagctct gggctggaaa gcggagcggg
     1681 acctgctcgc gatgatgggg accgcatggg cctgggagcg ccaacggtcc gaagaaaaat
     1741 gatagcctga tcgtagtaga tacgttcggc agcctgcccg aggtcggcca atgcgctccg
     1801 cctggtcctg acctgccttg ctgcacggac gaatttcaca ggggaccttc ccaaggttcc
     1861 ccattggatc catcggaccg gttcggcggg ccgctcatgg ccacgcattg gacctccgta
     1921 ccaagtgccc gaaggaatgg aaatggcggg tcccgatcgc tgaaaacagg atgaatgata
     1981 ccagcttgtt gtagcgctcc tcgcatcgcc ggagcggcta cgcaaaggcg cagtcggcag
     2041 gctcagcgtg acagctcctc gggagtgttc aaggacggtg ttcttgcaca ctcttgtccc
     2101 cactgtcacg ctgagcctgc cgaagcgcca tactcctgtt ctccgcactc gtcccggaac
     2161 ttggcgttat cccgcgctat aaggtttgat gtacgggcac ttctgttttt ggatcgggtg
     2221 gcccgccctt catctcccat gcccagcaat gaagtgttca gcgcggcctg gaagcgctga
     2281 ggatcgcgtg atcggatctg aactgttggt tttgaccaca agtactcacg ctcgcggatc
     2341 gctttgggtg tcccgatcgc tgaaatcagg ggtacggagg gctggtcatg gccatgcatt
     2401 ggacctccct gccgatcgcc caagggcatg aagaacgaag gaccagatga accacgcgaa
     2461 ccggttcgtc gagttcaatc tgacatggag aaggaagtcc aacgaagggc ccatgatcgg
     2521 accggatacc ggttacgtgc ttgatcatga ggctggaaga accaatgagc cggcacggtc
     2581 atcattatgg gcgcggtggg cgcatcagtc gtgcgtgcag tgcggcccag ggtcgatgat
     2641 gcaagaggag atcgcgggtc atgaatcgcc gaagccttct ccacgcgttg gtcagttgtt
     2701 cgggagacgg cggtggcact tgtgcccgca tgttgcggta cagttcgatg tagccttggt
     2761 gcacccgctc gggatccctt gtggcggacg ggtgcccaac gcggtagtgc agcacctcgc
     2821 gggccgtgct cacgtaccgg ccctgacgtg cgatgcccag gaaaaaggcg cagtcctccg
     2881 aatggtccat gtgttcgggc aggcgataat cataccccgg aaggcggcgc accatccatg
     2941 tgttcccggc gaagcaggag ccgtccagcg ccatgagcgc atcgaaaggc atgcctacga
     3001 accaaggtga gcgcacccag agggtcttcc ctgtgcgatg atcgaatccg cgcatggccc
     3061 catcggcgaa acgcgcctcc ggatagcgca ggaagaggtc atggcgaagg cgcaatgcgt
     3121 cctggggcag ccggtcgtcc gcgtccagga aacagaagaa cccgcccttc atggcggcaa
     3181 gacctttgtt ccgggccggg gatacgcccc ggcgggattc cgaaaatacg ctgatgcggg
     3241 gatcggaata ggaggtcgcg atcgcgtgcg tgccatctgt cgaggcattg tccacgatca
     3301 acagctccca gttcgaccac gtttgcgcga gcaccgaatc gatgcactcc cgaaggtaag
     3361 gcgccgcatt gcgggcgggc aggatgatcg agatcagcgg accggcggcg ttggtcacca
     3421 gcaacctcgg gtccacgggc cgttgtgtcc gggtcgtttc cgcgttcatg gtgctggtgg
     3481 tgatgcgctc ttcaggaaga tggtgcgcag gaatccgaag tccgacgggc gcaacaggaa
     3541 cagttcggcc acgggcatgt tcagacggta ccggagcacg aggcaggtca cgcccaggat
     3601 ggc
//
LOCUS       DAOQOC010000154         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12380_length_3601_cov_3.805133, whole genome shotgun sequence.
ACCESSION   DAOQOC010000154 DAOQOC010000000
VERSION     DAOQOC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12380_length_3601_cov_3.805133"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PL106_03530"
     CDS             <1..1282
                     /locus_tag="PL106_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNA32170.1"
                     /translation="VPGPWGRYAGQEITGSLFFDADGDGDQDLLALAGGNEVDLREPV
                     YTQRLYLNNGKAVFSEASGNLPPMMTSALRATAADIDGDGDMDLFIAGRTTPAHYPAP
                     PRSYLLVNDGRGRFVDATQALAPEAMGPGLVTDCRFTDLDGDKDADLLVSGEWMKLTW
                     FRNDGGHFIDASKDAGLEDTHGWWYSCATADVNGDGHMDIIGGNLGWNSKFHATKEHP
                     LHVYRADFDDNGRSDIVLAKEKNGTQLPVRGRECSSQQCPMILDKFPTYAEFANSDLA
                     GIYTQEKLAKALHLMATDMLSTVFLNDGKGHFTAKPLPMLAQTAPINAIIPMDLDGDG
                     RLDLVCAGNNWGAEVETARYDAGTGLVLRGDGKGGFTPMPIVASGLFAWGNVKDLALL
                     RTGPTKDPLIVVANNNDVLQAFAKKRAVSGLSMR"
     gene            complement(1332..2363)
                     /locus_tag="PL106_03535"
     CDS             complement(1332..2363)
                     /locus_tag="PL106_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA32171.1"
                     /translation="MDLRTPHRIALLIAVCSALAVALGFSVFGRFHGLSHAWLPFAIL
                     GPVTGAATYVIAVLLIRRFVQDRVDLIHRTIHEARTGTSSTVQDLDPLGRVNEEVVAW
                     ADSKRTEITELKERERFRREFMGNLAHELRTPIFSIQGYILTLLDGGLEDVRVNRDFL
                     ERANNGVERLIRIVEDLDMITELESGVMAMEPACTDLERTVDEAIESLEMLAREKKLI
                     LRNELDEGTLVLGDHDRLLQVFVNLFNNAVNYGHEGGLCTVRSFVVGDHVRVEVADNG
                     SGIAPEHLPRLFERFYRVGKSRSRNEGGSGLGLAIVKHIVESHGGTITVDSTEGKGTT
                     FAITLPKYK"
     gene            complement(2546..3085)
                     /locus_tag="PL106_03540"
     CDS             complement(2546..3085)
                     /locus_tag="PL106_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32172.1"
                     /translation="MSAKFVHRIGIVVSFVVLFAWTGCLKREEFPVEPILTFKAVEQF
                     HELQPQGVTPERFLNVIVDFTDGDGDIGLDPGDTLAPFGLGDAHYMNARHIFERLENG
                     VWTGVASEDSSRIKRITPSGQDPTLNGEIKRKIGPYPGARPGLPVINTGDTMRVKMWL
                     EDRALHRSNTVVSGPFILQ"
     gene            3113..>3601
                     /locus_tag="PL106_03545"
     CDS             3113..>3601
                     /locus_tag="PL106_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNA32173.1"
                     /translation="MIPIPSDLQDRPFVIGNDAAFRSLALDLFHLHAARNPVYRAFLN
                     ALGRDPGGVRTLEDIPFLPISTFRDQKVLLEGLEPAVTFTSSGTTGTTTARHAVPWPA
                     LYDRSFLTSFQAVYGHPSGWRILALLPAYLEREGSSLVHMARVLIELSGDPLSGTYLY
                     KYD"
BASE COUNT          699 a         1187 c         1057 g          658 t
ORIGIN      
        1 ggtacccggt ccatgggggc gttatgcggg ccaggagatc acgggcagcc tcttcttcga
       61 tgccgatggc gacggcgacc aggacctgct cgcactcgcg ggcggcaacg aggtggacct
      121 gcgcgagccg gtgtacaccc agcggctgta cctcaataac ggcaaggccg ttttctccga
      181 ggccagtggc aacctgccgc ccatgatgac cagcgcgctg cgcgcgaccg cggccgatat
      241 cgatggcgac ggcgacatgg acctcttcat cgctgggcgc accacacctg cgcactatcc
      301 ggccccaccc cgcagttact tgttggtgaa tgacggccgc ggccgtttcg tggatgccac
      361 gcaagctttg gcacctgagg ccatgggacc gggactcgtc accgattgcc ggttcaccga
      421 cctggatggg gacaaggatg cggacctgct cgtttcgggc gagtggatga agctgacctg
      481 gttcaggaac gacggagggc acttcatcga tgccagcaag gacgccggat tggaggatac
      541 ccacggctgg tggtacagtt gcgccacggc cgacgtgaac ggggatggtc acatggacat
      601 catcggaggg aaccttggat ggaacagcaa gttccacgcg acgaaggagc acccgctcca
      661 tgtgtaccgg gcggatttcg acgacaatgg gcgcagcgat atcgtactgg ccaaagagaa
      721 gaatgggacc cagctccccg tgcgcgggcg cgaatgcagc agccagcaat gcccgatgat
      781 ccttgacaag ttcccgacct acgccgagtt cgccaacagc gacctggcgg gcatctacac
      841 gcaggagaag ctggcgaagg cgctccatct gatggccacg gacatgctct ccacggtgtt
      901 cctcaacgat ggcaagggcc atttcaccgc gaagcccctg cccatgctgg cgcagaccgc
      961 acccatcaac gccatcatac cgatggacct ggatggcgat ggccgcctgg acctggtatg
     1021 cgcgggcaac aactggggcg ccgaagtgga aacggcacgc tacgatgcgg gcacaggact
     1081 ggtgcttcgc ggcgacggca agggcggctt cacaccgatg ccgatcgtcg ccagcggcct
     1141 gttcgcctgg gggaatgtga aagacctcgc gcttctccgc accggcccca ccaaggaccc
     1201 attgatcgtg gtggccaaca acaacgatgt gctgcaagcc ttcgccaaga agcgggcggt
     1261 gagcgggctt tcgatgaggt gacccggaca atgaccggcc gcgcaccggt ccaccgtgcc
     1321 gaccgccgga actatttgta cttcggaagt gtgatggcga acgtcgtccc cttcccttcg
     1381 gtactgtcaa ccgtgatggt gccaccatgg gactcaacga tgtgcttgac gatggccaat
     1441 cccaatccgc tgccaccctc attgcgcgac cggcttttgc ccaccctgta gaaccgctcg
     1501 aacaggcggg ggagatgctc cggagcgatg cccgatccat tgtcggccac ctccacgcgc
     1561 acatgatcac ccacgacgaa ggagcgcacc gtgcacagcc ctccctcatg cccgtaattc
     1621 acggcattgt tgaacaggtt cacgaacacc tgcagcaacc ggtcgtggtc ccccagcacg
     1681 agcgtgccct catccagttc attgcgcagg atcagcttct tctcccgcgc cagcatctcc
     1741 agcgattcga tggcttcgtc caccgtgcgt tcaaggtccg tgcatgccgg ttccatggcc
     1801 atcactccgc tctccaactc ggtgatcatg tccaggtcct ccacgatgcg gatcaaccgt
     1861 tccaccccgt tgttcgcgcg ttccaggaag tcgcgattga cccggacatc ctccaatccc
     1921 ccgtccagca gcgtgagtat gtagccctgt atgctgaaga tcggtgtgcg caactcgtgc
     1981 gccagattgc ccatgaactc cctccggaag cgttcacgct ctttcaattc cgtaatttcg
     2041 gtacgcttcg aatcagccca ggcgaccacc tcctcattca cccggcccaa cggatccagg
     2101 tcctgcacgg tggaggatgt cccggtccga gcttcatgga tcgtacggtg gatcaggtcg
     2161 acccggtcct gcacgaagcg cctgatcaac aacacggcaa taacgtatgt cgccgctccg
     2221 gtgaccggcc cgagtatcgc gaagggcaac caggcgtgcg aaaggccgtg gaaccggcca
     2281 aatacggaga agcccaaagc cacggcgagc gccgagcaca cggcgatgag gagggcgatc
     2341 cgatgcggtg tgcggagatc catggtaggc caagataggt gcgctaccga ccggtcatga
     2401 gcacttgtac cataccaacc ggctccggca tgtacaggcc ggggcacagg aatgccccgg
     2461 gctgtatgcg atcctcgtcc cccaagtcat gcggaacgga gaatggatcg cgccggagcc
     2521 atcggcaacc ggtcatataa ttccactact gcagtatgaa cgggccgctc accacggtgt
     2581 tgctccgatg gagcgcccgg tcctccagcc acattttcac acgcatggta tcgccggtat
     2641 tgatcacggg caggccgggg cgtgcgcccg gatagggtcc gatcttgcgc ttgatctccc
     2701 cgttgagcgt gggatcctgg ccgctcggtg taatgcgctt gatccggctc gagtcctccg
     2761 acgccacccc ggtccaaacg ccgttctcca agcgctcgaa gatgtgtcgt gcgttcatgt
     2821 aatgcgcatc gcccaaaccg aacggagcga gtgtgtcgcc cgggtccagc ccgatatcgc
     2881 cgtcgccgtc cgtgaaatcc actatgacat tgaggaaccg ctccggggtc acgccctggg
     2941 gttgaagttc atggaactgc tccaccgcct tgaaggtgag tatcggttcc accgggaatt
     3001 cctcccgctt caagcaaccc gtccatgcga acaggacaac aaaggatacc acgatcccga
     3061 tccggtgtac gaatttcgcg ctcatcgacc tcaaatgtag cacagcttgg ccgtgatccc
     3121 catcccctcc gatctgcagg accgcccctt cgtgatcggg aacgatgcgg cgttccggtc
     3181 cttggcgctg gatctgttcc acctgcacgc cgcacggaac cccgtttatc gtgccttctt
     3241 gaacgcgttg ggccgcgacc ccggcggtgt acgcacgttg gaggacatcc cattcctgcc
     3301 catctcgacc ttccgcgacc agaaagtgct gcttgagggt ctggaaccgg cggtcacgtt
     3361 caccagcagc ggcaccacgg gcaccaccac cgctcgccac gctgttccat ggcccgcgtt
     3421 gtacgatcgc tccttcctca cttcgttcca ggcggtatat ggccatccat ccggatggcg
     3481 catcctcgca ctgctgcccg cgtacctcga gcgcgagggc agctcgctcg ttcacatggc
     3541 ccgcgtattg atcgaactgt ccggcgatcc gctcagcggc acctacctgt acaagtacga
     3601 t
//
LOCUS       DAOQOC010000155         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12422_length_3593_cov_2.549463, whole genome shotgun sequence.
ACCESSION   DAOQOC010000155 DAOQOC010000000
VERSION     DAOQOC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12422_length_3593_cov_2.549463"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PL106_03550"
     CDS             <1..1534
                     /locus_tag="PL106_03550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA32174.1"
                     /translation="DTTAGPWLGELLRGLRSPEGLATVDPGSALRGTLRPYQQTGLRW
                     LHLLSHLGLGACLADDMGLGKTIQVLSLLLVRKRENKSAVPSLLVAPASLLANWAAEI
                     DRFAPGLRKRIAHPSAMPGDEWRSLDAHKLKGVDLVITSYGTLLRAPWIESTTWDLVI
                     IDEAQAIKNPGTKQTKAVKKVKAHARIALTGTPVENRLSDLWSLFDFVNPGLLGSSKQ
                     FSAYAKGLTSGTNASYAPLRELVRPYILRRMKTDRSIISDLPDKTEMKAYCQLSAKQA
                     ALYQKAVDELAGKLQEVDGIQRRGLVLSFLMRFKQICNHPSQWLGDNTWAEEASGKWA
                     RLRAIAETVASKQEKMLLFTQFRETTGPLAAFLGELFGRPGLVLHGDTPVKQRGELVK
                     RFQNEDTLPFFVLSLKAGGSGLNLTAASHVVHFDRWWNPAVEDQATDRAFRIGQKRNV
                     LIHKFICKGTVEDHIDELIDSKQKLSRALLQGGAELDITGLKDDELLKLFALDLSAAT
                     ND"
     gene            1538..2641
                     /locus_tag="PL106_03555"
     CDS             1538..2641
                     /locus_tag="PL106_03555"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNA32175.1"
                     /translation="MSFYNWKPYVSAAERKRKAEQKLLKMNKGGAELQPVRIEGQKIA
                     TTFWGKAWCQNMERYSDFDSRLPRGRTYVRNGSVLDLRIEPGKVAAMVSGSDLYQVQI
                     ALQPVEDARWKAICKDCSGSIDSLVELLQGKLSTAVMERICRPGTGLFPAPAELKFSC
                     SCPDWADMCKHVAAVFYGIGARLDEQPELLFLLRQVNEKELIAKAAALPVPAPGKAAR
                     NVLVNDDLSALFGLEMETAPAHTEPVSKATRMGKGEQQASVPKAKRTARKAPARKKVP
                     AKGATPKGRSALAPKKNAVSTARKGRKASTRTAKKKTKPVKAGWAEVVITDPLGLAGR
                     AKVVVLPGAVQVGRSPRKPIAAKVASVSGKRKK"
     gene            complement(2671..>3593)
                     /locus_tag="PL106_03560"
     CDS             complement(2671..>3593)
                     /locus_tag="PL106_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32176.1"
                     /translation="RGTWQAFHDPQRDRFTAPFRMTTLRIAGVPEPYNLPWHLGLERR
                     SFIRAKIALKWRTVPQGTGAMCELLRKGAIDLAVLVTEGAVRDILCGNPSRIVSPFVN
                     TPLTWGVHIGAHTALRSPDDLRGVPFAISRFNSGSHLMSLAYARERGWELAEKDLIVV
                     NDLAGAVKRLQEPKPAIFLWEKFITASHVHAGTLRRVDEYRPSWPCFVVVARNKVLEE
                     HGDAVQRLMNVVRDQARGLMEKTSAPEMVAQRYGMSLADAQEWFGNVRWNTEGRVDGD
                     MLRRVARALQNQGSLPQEVDTSDPGRVGAV"
BASE COUNT          745 a         1150 c         1103 g          595 t
ORIGIN      
        1 cgacacaacg gcggggccat ggctgggaga gctgctgcgc ggtttgcgat cacccgaagg
       61 tctcgctacc gtggatccgg gaagcgcgtt gcgcggcacc ctccgcccgt accagcagac
      121 cggcctgcgt tggctgcatc tgctttcgca tctgggcctt ggcgcctgcc tggccgatga
      181 catgggcctg ggcaagacca tccaggtgct ttcgcttctg ctcgtgcgca aacgcgagaa
      241 caagtcggcc gttcccagcc tgctcgtagc acccgcttcg ctactggcca actgggcggc
      301 ggagatcgac cgtttcgccc cgggactgcg caagcgcatc gcgcatcctt cggccatgcc
      361 gggcgatgaa tggcgcagcc tcgatgccca caagctgaag ggcgtcgatc tggtgatcac
      421 cagctatggc accttgctgc gcgcgccttg gatcgagagc accacgtggg acctggtgat
      481 cattgatgaa gcgcaggcga tcaagaaccc cggcaccaag cagaccaagg cggtgaaaaa
      541 ggtgaaggcc cacgcgcgca tcgccctcac cggcacgccc gtggagaacc gcctcagcga
      601 tctctggtcg cttttcgatt tcgtgaaccc gggcctgctg ggttcctcca agcagttcag
      661 cgcgtacgcc aaggggctga cctcgggcac caacgccagc tatgccccgc tgcgcgaact
      721 ggtgcgcccc tacatcctgc gccgcatgaa gacggaccgg tcgatcatca gcgacctgcc
      781 ggacaagacc gagatgaaag cctactgcca gctgagcgcg aagcaggccg cactgtacca
      841 gaaggcggtg gatgagctgg cgggaaaact ccaagaggtg gacggcatcc aacggcgcgg
      901 actcgtgctc tccttcctca tgcgcttcaa gcagatctgc aaccatccct cgcaatggct
      961 gggcgacaat acctgggctg aggaagccag cggcaagtgg gcgcgcttgc gcgcgatcgc
     1021 cgaaacagtt gcgtccaagc aggagaaaat gctcctcttc acgcagttcc gcgaaaccac
     1081 gggcccgctc gcggccttcc tcggcgaact cttcggcagg cccggcctgg tgctgcatgg
     1141 cgataccccg gtgaagcagc gcggcgaact ggtaaagcgt ttccagaacg aggacacgct
     1201 gccgttcttc gtgctctccc tcaaggcggg cggttccggc ctcaacctca ccgccgcctc
     1261 gcacgtcgtt catttcgatc gctggtggaa ccccgccgtg gaggaccagg ccaccgaccg
     1321 cgccttccgc atcggccaga agcgcaatgt gctcatccac aagttcatct gcaaaggcac
     1381 cgtggaagac cacatcgacg aactgatcga ttccaagcag aagctctcgc gcgcgttgct
     1441 gcaaggcggg gccgagctgg acatcaccgg attgaaggat gatgaactgt tgaagctctt
     1501 cgcgctcgac cttagcgccg ccacaaacga ctgaagcatg tcattctaca attggaaacc
     1561 gtatgtgtcc gctgccgaac gcaagcgcaa ggcggagcag aaattgctga aaatgaacaa
     1621 gggtggagcc gaactccagc cggtgcggat cgaaggccag aagatcgcca ccaccttctg
     1681 gggcaaggcc tggtgccaga acatggaacg ctacagcgac tttgacagcc ggctaccgcg
     1741 cgggcgaacg tatgtgcgca acggctcggt gctcgatctg cggatcgaac ccggaaaggt
     1801 ggcagccatg gtgagcggtt cggacctgta ccaggtgcag atcgccctgc aacccgttga
     1861 ggacgcgcgc tggaaagcca tctgcaagga ctgttcaggc tccatcgatt cgttggtgga
     1921 actgctgcaa ggaaagctct ccacggcggt gatggagcgc atctgtcggc ccggcacagg
     1981 tctcttcccc gcacccgccg aactgaagtt cagttgcagc tgccccgact gggccgatat
     2041 gtgcaagcac gtcgcagcag tgttctatgg catcggtgcg cgcttggacg agcagccgga
     2101 actgctcttc ctgttgcggc aggtgaacga gaaggaactg atcgccaaag cagcggcgct
     2161 gccggtgccg gcgccaggga aagccgccag gaacgtgctt gtcaatgatg acctgtcggc
     2221 gctcttcggc ctggaaatgg agacggcacc agctcacact gaaccagtat caaaagcaac
     2281 gaggatgggg aagggcgaac aacaggcatc ggtgccgaaa gcgaagcgca ccgcgagaaa
     2341 ggctcctgcg aggaagaagg tgcccgccaa gggtgcgaca cccaaggggc gaagtgccct
     2401 cgcgccgaag aagaacgcgg tcagcacagc caggaagggc cgcaaagcgt ccacaaggac
     2461 cgccaagaag aaaacgaagc ccgtgaaagc gggatgggcc gaggtcgtca tcaccgatcc
     2521 gctcggactt gccggccggg caaaggtggt cgtgctgccg ggtgccgtgc aggtgggccg
     2581 cagcccacgg aagccgatag cggccaaggt agcctcagtt tccggaaagc ggaagaaata
     2641 gacacgtatc cgatcgatgc ggcatcggtc tcatacggca ccgacccggc ccggatcgga
     2701 ggtatccacc tcctgaggta aagatccctg gttttgcaac gcgcgagcca cccgcctgag
     2761 catatcgcca tccactctcc cctccgtatt ccatcgcacg ttgccgaacc actcctgcgc
     2821 atcagccagc gacatcccgt accgctgcgc caccatctcc ggcgcgctcg ttttttccat
     2881 cagtcctcgt gcctggtcgc gcaccacgtt catcaggcgt tgaacagcat caccgtgttc
     2941 ttcgagcacc ttgttgcgag ccaccaccac gaaacagggc cagctcggcc ggtattcgtc
     3001 cacacggcgc agcgttcccg catgcacgtg cgaggccgtg atgaacttct cccacagaaa
     3061 gatcgcgggc ttcggttcct gcaaccgctt caccgcacca gcgaggtcgt tcaccacgat
     3121 aagatccttc tcggcgagtt cccagccgcg ttcccttgcg taggccagcg acatcaagtg
     3181 ggatccagag ttgaaacggc tgatcgcgaa gggcacgccc cgaaggtcat cgggtgaccg
     3241 gagtgccgtg tgcgcgccga tatgcacccc ccaggtcagc ggagtattca cgaaaggcga
     3301 aacgatccgg ctcgggttgc cgcagaggat gtcccttacc gcgccctcgg tgaccagcac
     3361 cgccagatcg atcgcccctt tgcgcaagag ttcgcacatc gcgccggtgc cctggggaac
     3421 agtgcgccac ttcaatgcga tcttggcacg tatgaatgag cgacgctcaa ggcccaggtg
     3481 ccaaggcagg ttgtacggct ctggaacgcc agcgatgcga agcgtggtca tccggaacgg
     3541 cgccgtgaaa cgatcccgct gcgggtcatg gaacgcttgc catgtaccac ggc
//
LOCUS       DAOQOC010000156         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12525_length_3574_cov_3.598181, whole genome shotgun sequence.
ACCESSION   DAOQOC010000156 DAOQOC010000000
VERSION     DAOQOC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12525_length_3574_cov_3.598181"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..444
                     /locus_tag="PL106_03565"
     CDS             49..444
                     /locus_tag="PL106_03565"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA32177.1"
                     /translation="MRRRYTVHASARFASKHWVYLPRLYASAQGGSREIVFGGLLHRR
                     VGTDSRYTTDKTSSSFHAGVFYRWNDAIVPMIQVEYKRMITAGISYDVNISGLRNASR
                     YKGGMEVTLRYIGAFGDKRRHLPRRSSVN"
     gene            complement(475..1098)
                     /locus_tag="PL106_03570"
     CDS             complement(475..1098)
                     /locus_tag="PL106_03570"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HNA32178.1"
                     /translation="MKRLILLAFIAASGAGTAQDAAPSSDLVKKYLTGSFTNAARVVS
                     DSTPVVVDMELRRIWLREKKGIWLYEEQARRGKKGSPFLQGIHHLVQRNDSTWVLSEH
                     HLDSLHLFIGAYRDVARFNGVKPEDARPLPGCEVELHWHAGRFQGSTGEKPCTESGPG
                     STSMTITIDAAPEDLVWKHAPRGVADDPGFHFDRKRLQKATVKAVGQ"
     gene            complement(1113..3068)
                     /gene="ispG"
                     /locus_tag="PL106_03575"
     CDS             complement(1113..3068)
                     /gene="ispG"
                     /locus_tag="PL106_03575"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNA32179.1"
                     /translation="MSTSTLLRTDQYCPSLTRYERWRTREVRMGAIGIGGDHPIRVQS
                     MTTTDTMDTGATVAQSIRMIQAGCELVRITAPSIKEAENLALIKKRLRASGYDTPLVA
                     DIHFTPNAAEVAARIVEKVRVNPGNYADKKKFDTRTYTDGEYAAEMERIRERFVPLVR
                     ICKEHGTAMRIGTNHGSLSDRIMNRYGDTPLGMVESAFEFLRICRDENFHDIVLSMKA
                     SNTQVMVQAYRLLVHKMMALGWDYPLHLGVTEAGDGEDGRIKSAVGIGALLEDGLGDT
                     VRVSLTEEPEAEIPVAQALADRYSARRGHRPIPELHDMPIDPFSHARRPTREVLNIGA
                     RHVPVVIADLSTKEKINAASLFAYGYHYSVPLDKWNLTDQACDYLFIGDHHLAFEIPG
                     TLGIVQEHAAWLKDRNKPRHYPLLQVDDLLNNVPTHHDLNFVHATLDQLTDEVIGLLD
                     EDDSCVLLLDTMNQHGYAEQRAAFFRLMTRKGDVPVIIGRDHINLDELRLMLYSSTDM
                     GALLLDGLGDGVMICPEKCGSDDMVNRTAFGILQATRSRISKTEYISCPSCGRTLFDL
                     QETTARIRARTSHLKGVKIGIMGCIVNGPGEMADADYGYVGTGPGVITLYKEKTVVKR
                     NVPSANAVDELIALIKEGGDWVEMPRD"
     gene            3278..>3574
                     /locus_tag="PL106_03580"
     CDS             3278..>3574
                     /locus_tag="PL106_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNA32180.1"
                     /translation="MASFDVIITGRGLAGTVLSETLQERGLRVRVFDMPRTGRSSHVA
                     GGLVNPIVLRRAQPSWRASTMLAIAGAFYRELEQRYDERFWHPVNMAIIFPTTKD"
BASE COUNT          607 a         1156 c         1075 g          736 t
ORIGIN      
        1 tgaggtgcac ccgccttgcg gcagtgaacg ttcaccgatg atgacgtact gcgtcgtcgg
       61 tacacggttc atgcgtccgc gcggttcgct tcgaaacatt gggtctacct gccccggctc
      121 tacgccagcg cacaaggcgg ctcacgggag atcgtgttcg gcggcctgct ccatcggcgt
      181 gtgggcaccg atagccgcta caccacggac aagacctcgt cgtccttcca tgcgggcgtg
      241 ttctaccgct ggaatgacgc catcgtgccc atgatccagg tggaatacaa gcgcatgatc
      301 acggcgggga tcagttacga cgtgaacatt tcaggcctcc gcaacgcttc acggtacaag
      361 ggcggcatgg aggtcacact ccggtacatc ggtgcgttcg gcgacaaacg ccgtcatctg
      421 ccgcgccgtt cctcggtgaa ctgagccccc ggtacaccgt gcgtacccgc gcacttactg
      481 gcccactgct ttcacggtag ctttctggag ccgcttccgg tcgaagtgga agcccggatc
      541 atccgccaca ccccgagggg catgcttcca aacgagatcc tcgggagccg catcgatggt
      601 gatggtcatc gacgtggaac cgggtccgct ctccgtacag ggcttctcgc cggtacttcc
      661 ctggaagcgg ccggcgtgcc agtggagttc cacctcgcaa ccgggaagtg gtcgggcatc
      721 ctccggcttc acaccattga agcgcgctac gtcccggtac gcaccgatga acagatgcag
      781 gctgtccaga tgatgttcgc tcaacaccca ggtgctgtcg ttgcgctgca ccaggtgatg
      841 gatgccttgc aggaagggcg aacccttctt gccgcgccgg gcctgttcct cgtacaacca
      901 gatgcctttc ttctcacgca accagatgcg ccgcagctcc atgtcgacca cgacaggcgt
      961 gctgtcggac accacccttg cagcgttggt gaagctgccg gtcaggtatt ttttcacaag
     1021 gtcggaggac ggtgccgcat cctgcgcggt cccggccccg ctggccgcaa tgaacgcaag
     1081 taggatcagt cgcttcatga tgccgaaaat agtcagtcac ggggcatctc cacccagtcg
     1141 ccgccctcct tgatcagggc gatcagctca tccacggcgt tcgcgctggg cacgttgcgc
     1201 ttgaccaccg tcttctcctt gtacaaggtg atcacacccg gcccggtacc gacatagccg
     1261 tagtcggcat cggccatctc gccggggccg ttcacgatgc agcccatgat cccgatcttc
     1321 acgcctttga ggtggctggt gcgcgcacgg atcctcgcgg tggtctcctg caggtcgaac
     1381 agcgtacgcc cgcaactcgg acaactgata tactcggtct tgctgatcct ggaccgcgtg
     1441 gcctgcagga tcccgaacgc cgtgcggttc accatgtcgt cactgccgca tttctccggg
     1501 cagatcatca ccccatcgcc cagaccatcc agcaacagtg cacccatgtc ggtactgctg
     1561 tacagcatca accgcagctc atccaggttg atatgatcgc gtccgatgat caccggtacg
     1621 tcgcccttcc gggtcatgag cctgaagaaa gccgcacgct gctcggcgta accgtgctgg
     1681 ttcatcgtat cgagcagcag tacgcaacta tcgtcctcgt ccagtagccc gatcacctcg
     1741 tcggtcagct ggtcgagcgt ggcatgcacg aagttgaggt cgtggtgtgt gggcacgttg
     1801 ttcagcaggt cgtccacctg cagcaagggg tagtgccggg gtttgttccg gtccttgagc
     1861 cacgccgcgt gctcttgaac gatgcccagt gttcccggta tttcgaaggc gagatggtgg
     1921 tcgccgatga agaggtagtc gcacgcctga tcggtgaggt tccacttgtc cagcggcacg
     1981 ctgtagtgat agccgtaagc gaacagggaa gcggcgttga tcttctcctt cgtgctcagg
     2041 tcggcgatca ccaccggtac atgtcgcgca ccgatgttga gcacctccct ggtgggacgt
     2101 cgcgcatggc tgaagggatc gatcggcatg tcgtgcaact cggggatggg cctgtggccc
     2161 cgccgtgcgg agtaccggtc ggcgagcgcc tgggcaacag ggatctccgc ttccggctcc
     2221 tcggtgagcg acacccgcac cgtatcaccg aggccgtcct cgagcagggc ccctatgccc
     2281 accgcactct ttatccggcc atcctccccg tcccccgctt cggtgacgcc cagatgaagc
     2341 ggataatccc agcccagggc catcatcttg tgtacgagca ggcggtaggc ctgtaccatc
     2401 acttgcgtat tgctggcctt catgctcagc acgatgtcgt ggaagttctc gtcccgacag
     2461 atccgcagga attcgaaagc gctctccacc atgcccagcg gggtgtcccc gtacctgttc
     2521 atgatcctgt cgctcaggga tccatggttg gtgccgatgc gcatggccgt accatgttcc
     2581 ttgcagatcc gcaccagcgg gacgaagcgc tcccggatgc gttccatctc ggccgcgtat
     2641 tcaccatcgg tgtaggtgcg cgtatcgaac ttcttcttgt cggcgtagtt gccggggttc
     2701 accctcacct tctccacgat ccgcgcggcc acctcggcag cgttcggcgt aaaatggata
     2761 tcggccacca acggggtatc atatccggag gcgcgcaacc gcttcttgat cagcgccagg
     2821 ttctccgcct ccttgatgct gggtgccgtg atgcgcacca gttcacaacc cgcctggatc
     2881 atccggatgc tctgcgccac cgtggccccg gtatccatgg tgtccgtggt ggtcatgctc
     2941 tgcacacgga tcggatggtc gccaccgatg ccgatggcgc ccatccgtac ttcgcgggta
     3001 cgccagcgtt cgtaacgtgt gagcgagggg caatactgat cggtacgcag gagtgtgctg
     3061 gtggacatgt gcgtgtgcgg ttccgtcgtg tggcgaaggt agccgctgcg ccgcgatcgc
     3121 ggccgatcca tggtgtctcc gggcaggtgg gcacgtgatc ccatccgcgc cgcgatgtcc
     3181 gaccggagtc cacttgcacc ggcatgatga gcatggccga ccgcatggcg tgtgctggat
     3241 cgcaatggat ccggtgcgca ggcccgatat tcggcccgtg gcttcgttcg acgtcatcat
     3301 caccggtcgc ggacttgcgg gcaccgtact ttcagaaacg ttgcaggaac gcggcctgcg
     3361 ggtgcgtgtg ttcgatatgc ccaggacagg gcgctcctcc cacgtggccg gtgggctcgt
     3421 gaaccccatc gtcctgcggc gcgcgcaacc cagttggcgt gcctccacca tgctcgccat
     3481 cgccggtgcc ttttatcgcg agttggagca gcgctacgac gagcgcttct ggcacccggt
     3541 gaacatggcg atcatctttc ccacgaccaa ggat
//
LOCUS       DAOQOC010000157         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12622_length_3557_cov_4.221017, whole genome shotgun sequence.
ACCESSION   DAOQOC010000157 DAOQOC010000000
VERSION     DAOQOC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12622_length_3557_cov_4.221017"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PL106_03585"
     CDS             complement(<1..291)
                     /locus_tag="PL106_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32181.1"
                     /translation="MRYILSTGTWAMLFGTALLTIGCTKEEEETPSGPTGGTWSLNTV
                     QNGNVVTVKDSTNQNFTFTNDRTWLLWGFVKVQNGATLTIEPGTIIKGDKATK"
     gene            complement(562..3378)
                     /locus_tag="PL106_03590"
     CDS             complement(562..3378)
                     /locus_tag="PL106_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA32182.1"
                     /translation="MKRIPGLLPALLLCSLASAQRGAISGTVTAVENGRSEPQPFASV
                     SIKGTGTGANTDMEGKYYFKVEPGTWTVVSALVGRVPVERSVTVVADQTVTVDLHLDG
                     GVTEMRAVEVVKEKRVDTEAAVLMETRKSDQVVSGIGRQQIAKGQDRTAGDVVKRIPG
                     VTLIGDRFVMVRGLSDRYNNVMLNDAAAPGLEADKRAFSFDMVPSGALDRILIFKSAS
                     PELPGDFSGGVVKVSTLGVPDKNSVEVNYTLGLRPGTTFQDFHQSKGSPTDAIGFDNG
                     FRKLPNGFPANLYHVTDAEQLATLGRAMPNNWTASTRTAGPDHRMHLIIARRLGKKDG
                     ARFGMVNAINYSNTTVAYTARNHNYNAYDLNALRSDTVYSYMDDENIRTARLGVMSNW
                     SALLANGTKLEFRNLFNQIGEDRTTLRTGRNLEEGNEVRNSAFKYQQRVIYSGQLHGS
                     HELASDVSKLDWTLGYGKAVSKEPDHRRVRTVRDLGISDADTPFQTVVASSATTLDAG
                     RFFSELDETVLSGKLDHELRIPVKKEGLDPRILVGAYAERKDRVFAARWMSLRIANIT
                     WFDNSQLYLPLDEVFSAEHINSTTGFKLEEGTNPSDSYSGTNTLLAGFLSGQIGFSRK
                     WNVKGGARVEHNRQQLNSATYGGRPVGVDKKLLFVLPSVNASWNISERSVLRAGFGST
                     LNRPEFRELAPFAYYDFSANTVIYGNPALRTAAIQNTDARFEFYPSLTEVVSLGAFHK
                     HFADPIEMYYVPGTGGGGTRNFTYNNALSARSMGLEAEVRLSTARWAGEGFMGRWSAL
                     FNGTWIHSQVNLGATMGGQDDQRPMMGQSPWVLNAGLYYEDVAHKLRFNALFNVCGPR
                     LFAVGSIGVPDVYEMPRNVLDIALSKGFGNRFEVKVGVQDLLNQRMRLLQDGNSDGRL
                     DDTDEEVMSFRRGAYLSAGLSCRF"
BASE COUNT          633 a         1117 c         1064 g          743 t
ORIGIN      
        1 cttcgtggcc ttgtcgccct tgatgatggt acccggttca atggtgagtg tggctccgtt
       61 ctgcaccttc acaaagcccc agagcagcca ggtcctgtca ttggtgaacg tgaaattctg
      121 gttcgtgctg tccttgaccg tgaccacgtt cccgttctgc acggtgttca aagaccatgt
      181 gccgcccgtg gggccgcttg gcgtttcctc ttcttccttg gtgcagccga tcgtcagcag
      241 tgcggtgccg aacagcatcg cccacgtgcc cgtgctcaga atgtagcgca tgtttccttg
      301 ttgtttcatc atgccgcaaa gggaggtccc tgacatgatg ccggggttag caccgcttta
      361 ccaaatcctt tccacgcgtt atcatttcgt tacgtatgga acccgccgca aggagccatt
      421 gggcgagcgg ccctctcccg ggtccgaacg cggccgttca aggtcaccga tcacgggttc
      481 gtaccaccgc tgacggaacg aacatcctga aggaaacacg aacgggcagc gatcaggtgg
      541 aacccggccg tatacccgcc ttcagaatcg gcagctgaga ccggcgctga gataggcacc
      601 gcgtcgaaag ctcatcactt cctcgtccgt atcatccaat cggccgtcac tgttgccgtc
      661 ctgcaacagg cgcatgcgct ggttcaacag gtcctgcacg cccaccttca cctcgaaccg
      721 gttgccaaag cccttgctca acgcgatgtc cagcacgtta cgtggcatct cataaacgtc
      781 cggaacgccg atggagccca ctgcgaagag ccgcgggccg catacattga acaaggcatt
      841 gaaccggagc ttgtgtgcca catcctcgta gtagaggccc gcattcagca cccaaggact
      901 ctggcccatc atggggcgct gatcgtcctg cccgcccatg gtggcaccga ggttcacctg
      961 gctatggatc cacgtgccgt tgaagagcgc actccagcgg cccatgaagc cctctccggc
     1021 ccaccgtgcg gtggacaggc gcacctcggc ctcgaggccc atactgcgtg ctgataaggc
     1081 gttgttgtac gtgaagttcc gcgtgcctcc gcctcccgta ccgggcacat agtacatctc
     1141 gatagggtca gcgaagtgct tgtggaaggc gcccaggctc accacctcgg tcagcgaagg
     1201 gtagaactcg aagcgggcat cggtgttctg aatggcggcc gttcgcaatg cggggttgcc
     1261 atagatcacg gtgttggcac tgaaatcata gtatgcgaag ggcgcgagct cacggaactc
     1321 gggacggttg agcgtggagc cgaaacccgc acgcaggact gagcgttccg agatgttcca
     1381 cgaggcgttg accgagggca gtacgaagag caacttcttg tccaccccca ccggccggcc
     1441 gccataggtg gcgctgttga gttgctgacg gttgtgctcc acgcgcgcgc cgcctttcac
     1501 gttccacttc ctgctgaacc cgatctgccc ggacaggaat ccggccagca gggtgttcgt
     1561 cccggaatag ctgtcactgg ggttggtgcc ttcctccagc ttgaatcccg tggtggagtt
     1621 gatgtgttcc gcgctgaaca cctcgtccag cggcaggtac aattggctgt tgtcgaacca
     1681 ggtgatgttc gcgatgcgca gtgacatcca gcgtgcggcg aacacgcggt ccttgcgctc
     1741 ggcgtacgca cccacgagga tcctcgggtc gaggccctcc ttcttcacag ggatccgcag
     1801 ttcgtggtcc agtttgccgc tcagcacggt ctcgtccagt tcgctgaaga accggccggc
     1861 gtccaaggtt gtggcgctcg aggccacaac ggtctggaac ggggtgtccg catcggaaat
     1921 gcccaggtcg cgtacggtgc gtacacggcg atggtcgggt tccttgctga cggccttgcc
     1981 gtaaccgagc gtccagtcca gcttgctcac atcggacgcc agctcgtggg aaccgtgcaa
     2041 ctggccgctg tagatcacac gttgctggta tttgaatgcg ctattgcgca cctcgttccc
     2101 ctcttccagg ttccgaccgg tgcgcagcgt ggtgcggtcc tcgccgatct ggttgaagag
     2161 gttgcggaac tcgagcttgg taccgttagc aagcagtgca ctccagttgc tcatcacgcc
     2221 caatcgcgct gtccggatgt tctcatcatc catataactg tacaccgtgt cgctgcggag
     2281 tgcgttgagg tcgtaagcgt tgtaattgtg gttgcgcgcg gtgtatgcaa cggtggtatt
     2341 cgagtagttg atggcgttca ccatgccgaa acgtgcaccg tccttcttgc cgaggcggcg
     2401 ggcgatgatg agatgcatgc ggtggtcagg cccggcggta cgcgtggagg cagtccagtt
     2461 gttgggcatc gcgcgaccca gggtggccag ctgctccgca tccgtcacgt ggtagaggtt
     2521 ggccggaaat ccgttcggca gcttccggaa accattgtcg aagccgatcg catccgtcgg
     2581 gctccctttg ctctggtgga agtcctggaa ggtggtgccc gggcggagac caagggtgta
     2641 gttgacctcg acggagttct tgtccggcac acccaaggtg gagaccttca ccacaccgcc
     2701 cgagaaatcg ccgggcagtt cgggggaggc gctcttgaag atgagtatcc ggtccagcgc
     2761 cccgctgggg accatgtcga agctgaaggc ccgcttgtcg gcctccagcc caggtgcggc
     2821 ggcatcattg agcatcacat tgttatagcg gtccgagagg ccgcggacca tcacgaaacg
     2881 atcgccgatg agggtgacac cggggatgcg cttcaccacg tcaccggcgg tccggtcctg
     2941 gcccttggct atctgctggc gcccgatccc gctcaccacc tgatcgctct ttctggtctc
     3001 catcagcaca gcggcctcgg tgtccacgcg tttctccttc accacctcca cggcccgcat
     3061 ctcggtaacg ccaccgtcca ggtgaagatc gacggtcact gtctgatcgg ccaccaccgt
     3121 cacggagcgc tccacgggca cgcggcccac caaggccgac accaccgtcc aggtacccgg
     3181 ctccaccttg aaataatact taccctccat gtccgtattg gccccggtac ctgtgccctt
     3241 gatggacacg ctggcaaatg gttgaggttc ggaacggccg ttctccacag cggtcaccgt
     3301 tccggagatg gctcccctct gggcggatgc aagggagcac agcagcaacg ctgggagcag
     3361 gccaggaatg cgtttcatcg cgagcggtct tctgcccgca aaagtccctt ccgaaggtta
     3421 ggcgcgcctt gctcctgcgt tatcggtccg ttaaaccttc gacatcggac ttcacgatcg
     3481 tttaatgccg gtttcacgtc gcgcccgctg ctccaggaac caccatttcg gtcggtcctt
     3541 gcgaccctgg gccacct
//
LOCUS       DAOQOC010000158         3556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12631_length_3556_cov_4.898315, whole genome shotgun sequence.
ACCESSION   DAOQOC010000158 DAOQOC010000000
VERSION     DAOQOC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12631_length_3556_cov_4.898315"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PL106_03595"
     CDS             <1..190
                     /locus_tag="PL106_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNA32183.1"
                     /translation="NYRGSVSGEQVHYPIGSVGFIRGGSRFSLSYGRQRAGIFCVGGV
                     CRVVPASNGLTLSITSTF"
     gene            193..1056
                     /locus_tag="PL106_03600"
     CDS             193..1056
                     /locus_tag="PL106_03600"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNA32184.1"
                     /translation="MMSRTVLHPAFRTILAWGACALLVACDVVNDPHGDVSGPTPPTT
                     DGVPRRVLLEKFTGHTCPNCPEGDVIASELQAQYGSELLILTSIHQGFFAELHPPLYD
                     TDFTTPAGNDYGSIFTPPSYPQGMVDRRRVNNTWTFGRGDWDERIAEVIGQPAKVDVR
                     ITAAEVNSGAINTSVKVKLIEELDPVEYNLTVFLQESGIIDYQTDQNATPPDVPNYEH
                     NHILRGALNGSWGQPFAMTDLAVGDSTTVDLTNLAIPANVLSISNCELVAYLYRTDTY
                     EILQVHAFDLD"
     gene            1273..1467
                     /locus_tag="PL106_03605"
     CDS             1273..1467
                     /locus_tag="PL106_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32185.1"
                     /translation="MMELSKQVLQKVSFDSRLFKKELVKARRWLGQHDQLLLKAWCLA
                     TFGHQYRETIQEVFRQSMRT"
     gene            1995..3239
                     /locus_tag="PL106_03610"
     CDS             1995..3239
                     /locus_tag="PL106_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32186.1"
                     /translation="MRLPLLFARRYLLARRSTNAVNIITVISIVVVAVVTAAMVVVLS
                     TLNGIGELVDKLYSPFDQDVTITLQEGKTYPRDSLPLDRVKAVPGVAMVSWTIQDNVL
                     LRCGDKQAVATLKGVEPIYLDMAGLPGSMYEGQPRLRGDNGPTVILGAALKDQLAVPN
                     DDGVLRPLVISAPVRGRKLLRYREQAFERENVAVAGSFSINMDFDQRFAVAPIALADS
                     LLHYGGQVSALEIKAVPGTDIELLADRIGALVGPRFKVLSRYRKNELMYRTNAIEKRF
                     TFGVLAFIALIGAFNIIASLTLMMIEKQRDMRTLAGMGATASFVRRVFFAEGMLIVGV
                     GTVAGLLLGLFVCGIQLWTGVVRLEGSVVKSYPVQVMVSDLALIFLAVMAIGILATAT
                     SLMGLWRRSADGAVGYLQAARA"
     gene            complement(3221..>3556)
                     /locus_tag="PL106_03615"
     CDS             complement(3221..>3556)
                     /locus_tag="PL106_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNA32187.1"
                     /translation="DRYGVDGVMIGRASIGHPWIFNAIKHFMATGMHLSAPTLNDRVD
                     AAREHLERSIAWKGDWEGVVEMRRHYTNYFKGLPDFKEHRLRLVTERDPRIVLELLEV
                     VRAAYALAA"
BASE COUNT          678 a         1141 c         1061 g          676 t
ORIGIN      
        1 caattaccgt ggatcggtga gcggcgagca ggttcactac ccgatcggca gcgtgggctt
       61 catccgcggg ggcagccgat tcagcctgag ctatggccgc cagcgcgccg gcatcttctg
      121 cgtgggaggg gtctgccgcg tggtgcctgc gagcaacggc ctcaccctct ccatcaccag
      181 cacattctga acatgatgtc ccgaacggtt ctccatcccg catttcgtac catccttgcc
      241 tggggtgcat gcgcactgct ggtggcctgc gatgtggtga acgatccgca cggcgatgtc
      301 agcggcccca cgccgcccac aaccgatggc gtacctcgtc gggtgctgct tgagaaattc
      361 acggggcaca cctgccccaa ttgcccggag ggcgatgtga tcgcgtcgga gctgcaagcc
      421 caatacggta gtgagttgct catcctcacg agcatccacc aaggcttctt cgccgaactg
      481 cacccgccct tgtacgacac cgatttcacc acaccggccg gtaacgacta tggcagcatc
      541 ttcaccccgc cctcctatcc gcagggcatg gtggaccggc gacgcgtgaa caacacctgg
      601 accttcggcc gtggtgattg ggatgaacgc attgccgagg tgatcggtca accggccaaa
      661 gtggacgtgc gcatcaccgc cgccgaggtg aatagcggcg cgatcaacac gtcggtgaag
      721 gtgaaactga tcgaagaact cgaccccgtg gagtacaacc tcaccgtctt cctccaagag
      781 agcggcatca ttgattacca gaccgaccag aacgccacgc cacccgacgt gccgaactac
      841 gagcacaacc acattcttcg gggagcactc aacggctctt ggggccagcc cttcgccatg
      901 accgacttgg ctgttggcga ctccaccacg gtggacctca ccaacctggc catccccgcc
      961 aatgtcctga gtatcagcaa ctgcgagctg gtggcctacc tgtaccgcac cgatacgtac
     1021 gagatcctgc aggtacacgc cttcgacctg gattgaacct gatccgaccc cacatacgga
     1081 tccccttgac cggcccgttc taccaagaac ctccgtattg gtcggcggtc ggttgaaaag
     1141 tccaattccg cgatcgaaca ccgccatcgg agaaagatgt taccttcatc cggcttttcc
     1201 ggagactctt cccctcacca ttcccctcac ggggaaaatt gaagaaacgt gatgatggac
     1261 caaggaagga agatgatgga gctcagcaaa caggtgctcc aaaaggtgag cttcgatagc
     1321 cgccttttca agaaagaact ggtgaaggcc cgccgttggt tgggccagca cgatcaattg
     1381 ctcctcaaag cctggtgttt ggccaccttc ggccatcagt accgggagac catccaggag
     1441 gtgttccggc agagcatgcg gacgtgagtt gcacgttggc ggcctacggc tgaacagacc
     1501 gatcacgtcg ggggtgcctc caaggacatg ttgttccccg aaccggcgtt ggcggcccaa
     1561 ggtccagggc gtgttgctcg gattcggcac ctggttccgt gcttcgtacc ggacagcaaa
     1621 agtgcggtcc gcccacagcg gaacagcctg ctggggtcgc cccacgaaat tttggcgctg
     1681 catctttgac cgccattgag tccacatggg cgtgcccgga acctacctga tgcccttctg
     1741 gatcggacac acgttccgct tcgacgcgct ttccggcgat cgtcgggata gtgatcgggc
     1801 caggccacgg gcccatgcag ttccattgaa agattccttt ggatcgatgg tggatcgaac
     1861 ggtgatgctt tgcggaacgt ggccagcacg taagccggca ggccgttcga cccggcacga
     1921 gcatcggaac gccagcaata ccgggcaacg ggcatcatcg cgcatgaccg gggcgcgcat
     1981 ctccggctga tcacatgcgc ctgcctctcc tcttcgcacg ccgctacctg ctggccaggc
     2041 gcagcaccaa cgccgtgaac atcatcacgg tgatcagcat cgttgtggta gcggtggtga
     2101 ccgccgccat ggtggtggtg ctcagcaccc tcaacggcat cggcgagctg gtggacaagc
     2161 tctacagccc cttcgatcag gatgtcacca tcaccctgca agagggaaaa acatacccgc
     2221 gcgacagctt gccacttgat cgggtaaaag cggtgcccgg tgtggccatg gtcagttgga
     2281 cgatccagga caatgtgctg ctgcgatgcg gcgacaagca ggcggtggcc accctgaaag
     2341 gcgtggagcc catctacctc gacatggcgg ggctgcccgg cagcatgtac gaggggcagc
     2401 cccgccttcg cggggacaat ggacccaccg tgatcctggg cgcggcgctc aaggaccagc
     2461 tcgcggtgcc caacgacgac ggcgtgcttc ggccccttgt gatcagcgca cccgtgcgcg
     2521 gccgcaaact gctgcgatac agggaacagg cctttgagcg ggagaacgtg gccgtggccg
     2581 gttccttctc catcaacatg gacttcgacc aacgcttcgc cgtggcaccc atcgcccttg
     2641 cggactccct gctccattat ggcgggcaag tcagcgcact ggagatcaaa gccgttccag
     2701 gaaccgacat tgagttgctt gccgaccgca tcggagcgct cgtcgggccg cggttcaagg
     2761 tgctctcgcg ctaccgcaag aacgagttga tgtaccgcac caacgccatc gagaaacgct
     2821 tcacattcgg agtgctcgcc ttcatcgccc tcatcggcgc cttcaacatc atcgcttcac
     2881 tcacgctcat gatgatcgag aagcaacgcg atatgcgcac cctggcgggt atgggggcta
     2941 ccgcatcgtt cgtccggcgc gtgttcttcg ccgagggcat gctcatcgtg ggtgtgggca
     3001 ccgtggcggg cctgctgctc ggcctgttcg tgtgcgggat ccaactgtgg accggcgtgg
     3061 tgcgactgga gggatccgtg gttaaaagct accctgtgca agtgatggta tctgacctgg
     3121 ccctcatttt cctggcggtg atggccatcg gcatcctggc caccgccacc tcgttgatgg
     3181 gtctgtggcg gcgatcggcc gatggggccg ttggctacct tcaggctgcg agagcgtagg
     3241 ctgcgcgtac cacctcgagc aactccagca cgatgcgcgg gtcgcgctcg gtcaccagcc
     3301 gcagccggtg ctccttgaaa tcaggcaggc ccttgaagta gttggtgtag tggcggcgca
     3361 tctccaccac gccttcccag tcgcctttcc acgcgatgct gcgctccaga tgctcgcgtg
     3421 ccgcgtccac acggtcgttc agcgtgggcg ccgacaggtg catgccggtg gccatgaagt
     3481 gcttgatggc gttgaagatc cacgggtgtc cgatgctcgc gcggccgatc atcaccccgt
     3541 ccacaccgta ccgatc
//
LOCUS       DAOQOC010000159         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12640_length_3555_cov_3.414286, whole genome shotgun sequence.
ACCESSION   DAOQOC010000159 DAOQOC010000000
VERSION     DAOQOC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12640_length_3555_cov_3.414286"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="PL106_03620"
     CDS             complement(<1..1938)
                     /locus_tag="PL106_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA32188.1"
                     /translation="MRRSRAPFLALLTFLLLTLNASAQYELRGTVTDKATGETVIGAV
                     VKVKGEAKGVSTDLDGKFTLKVTQLPPFVIVITSMGYAAQEIAVASLDEPVKARMGPD
                     EVMLREAVIVKERISEKQKQAPLTVESMDVIAIKEAPSGDFYESLGTLKGVDMTAASM
                     GFKVINTRGFNSTSPVRSLQLIDGVDNQSPGLNFSLGNFLGASDLDVMKVDIIAGAST
                     AFYGPGAFNGVVSMSTKSPWTFPGLSVSVKGGERDLLEGAVRWAQVFKDKEGRDKFAY
                     KLNLFALRATDWYAQNYGATGNSPTTVANAGGYDGVNIYGDENVTVNNDYTRSLFERR
                     NSPGLGIFLRPGYKESDLVDYGTDNIKAGTSVHYKLTDDIEVIGAGSYSRGSTVYHGE
                     NRYRLKNIQFHQERLEIRQKDKWYVRGYHTGEDAGNTYDIFTTALRLQDASGATSRWN
                     TAYYNLWQTWIKGPGTGGVPGLLQSQTGWYQPGQEATDSLGPGTTAETYDAYLTGFIN
                     SHPDLFQYYHQLVTDSVNNTNDTYLDPAYVPGTQRFDDAFNAITGKLFTEGGSRFYDR
                     SRLVHAMGEYRFKPGFGEISVGGNFRQYMPDSRGTIFKDTAGVKIRNREYGLFVGLEK
                     PFVDSTLKMTATLRMDHNQNFD"
     gene            2026..3426
                     /locus_tag="PL106_03625"
     CDS             2026..3426
                     /locus_tag="PL106_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNA32189.1"
                     /translation="MSQFHRSLRLLDAVMLVAGSMIGSGIFIVSAGMLRGLGSPAWLL
                     TAWVVAGVLTVLAALSYGELAAMMPEAGGQFVYLRRAYGKLTSFMYGWTAFTVITSGI
                     IAAVAVAFAKFSAVFFPVLSPDNILLILGPVHVSAAQVYAVCAILLLTWFNTRGVEHS
                     KTFTTVFTLAKVAAVLALVVGGLAVGLGTDLLSTNFSRGWEAVRTGSDGALTPLTGMA
                     LLSALGVAMVGSLFSSDGWNSVTFIAGEIREPQRNIPLSLFLGTFMVTALYVLANITY
                     LSLLPVEGIQGAAFDRVGTAAAEVIMGPAAVGVMAALIMVSTFGCINGYVLVGPRMYY
                     AMAREGVFFKKAATLNARGVPAWATWLQCAWACLLCFSGTYNDLLDYTTFASLLFYVV
                     TIGGVFILRKREPDTPRPYRAFGYPLVPAIYMLCALAFCVNLLVMQPLFAGLGLGIVA
                     LGAVVYFATGMQRSGS"
BASE COUNT          522 a         1165 c         1092 g          776 t
ORIGIN      
        1 gtcgaagttc tggttgtggt ccatccgcag ggtggccgtc atcttcagtg tgctgtccac
       61 gaagggcttc tccaggccca cgaacaggcc atactcgcgg ttgcggatct ttacgccggc
      121 cgtgtccttg aagatggtgc cccggctgtc gggcatgtac tgccggaagt tgccgcccac
      181 gctgatctcg ccgaaccccg gcttgaagcg gtactcgccc atcgcatgca ccagtcgcga
      241 ccggtcgtag aaccgtgatc caccttcggt gaagagcttg cccgtgatcg cgttgaacgc
      301 gtcgtcgaag cgttgcgtgc ccggtacgta cgcgggatcc agataggtat cgttggtgtt
      361 gttcacggag tcggtcacca actggtggta gtattggaag aggtccgggt ggctgttgat
      421 gaagcccgtg aggtacgcgt cgtaggtctc ggcggtggtg cccggcccca agctgtcggt
      481 ggcttcctgg cccggctggt accatcccgt ctggctttgc agcagccccg gcacaccgcc
      541 cgtgcccggc cctttgatcc aggtctgcca caagttgtag taggccgtgt tccagcggct
      601 ggtggcgccg gatgcgtcct ggaggcgcaa cgcggtggtg aagatgtcgt aggtgttgcc
      661 cgcatcctca ccggtatgat acccgcgcac gtaccatttg tccttctgcc ggatctccag
      721 ccgctcctgg tggaactgga tgttcttcag gcggtagcgg ttctcgccgt ggtacaccgt
      781 gctgcccctg ctgtagctgc ccgcaccgat cacctcgatg tcgtcggtga gtttgtaatg
      841 caccgatgtg cccgccttga tattatcggt accgtagtcc accaggtcgc tctccttgta
      901 cccgggccgc agaaaaatac cgagtccggg ggagttacgc cgctcgaaca ggctgcgcgt
      961 gtagtcgttg ttcactgtca cgttctcgtc gccgtagatg ttcactccat cgtagccgcc
     1021 ggcattggcc accgtggtgg gcgagttgcc ggtggcgccg tagttctgcg cgtaccagtc
     1081 ggtggcccgc agggcgaaga ggttcagctt gtaggcgaat ttgtccctgc cctccttgtc
     1141 cttgaagacc tgcgcccagc gcaccgctcc ttccagcagg tcgcgctcgc cgcctttcac
     1201 gctcacgctc aggccgggga acgtccacgg gctcttggtg ctcatgctca ccacaccgtt
     1261 gaaggcgccc ggaccgtaaa aggccgtgct ggccccggcg atgatgtcca ccttcatcac
     1321 gtccaggtcc gaggcgccca ggaagttgcc cagcgagaag ttgagccccg gactctggtt
     1381 gtccacgccg tcgatcagct gcagggaccg caccggactg gtgctgttga atccccgcgt
     1441 gttgatcacc ttgaacccca tgctggccgc ggtcatgtcc acgcccttca acgtaccgag
     1501 actttcgtaa aaatcgccgc tcggggcctc cttgatcgcg atcacgtcca tgctctccac
     1561 cgtgagcggc gcctgcttct gcttctcgct gatgcgctcc ttcacgatca cggcctcgcg
     1621 cagcatcacc tcgtcggggc ccatccgtgc tttcacgggt tcgtccaggc tggccaccgc
     1681 gatctcctgc gcggcatagc ccatgctggt gatcacgatg acgaagggcg ggagctgcgt
     1741 caccttcaat gtgaatttcc cgtccaggtc cgtgctcaca cccttggctt cgcccttcac
     1801 cttcaccacc gccccgatca ccgtttcgcc ggtggccttg tcggtgacgg taccgcgcaa
     1861 ctcgtactgg gccgaggcgt tgagggtgag gagcaggaag gtcaacagcg ccaggaacgg
     1921 cgcgcggctt cggcgcatgg tgggtgatcg gacggccttc ggggcacggc gtgcgccaat
     1981 atagatggtg gcatccttct acattcggcg cctttccatc ccaccatgtc ccaattccac
     2041 cgttcgctcc gcctgctgga tgccgtgatg ctcgtggccg gctccatgat cggcagcggt
     2101 atcttcatcg tgagtgctgg catgctgcgc ggtctgggct cgcccgcctg gctgcttacc
     2161 gcctgggtgg tcgcgggggt actcaccgtt ctggccgccc tcagctatgg cgagctcgcc
     2221 gctatgatgc ccgaagcggg cggccagttc gtgtacctgc gccgcgcgta tggaaaactc
     2281 acttctttca tgtatgggtg gaccgccttc accgtgatca cctctggcat catcgccgcc
     2341 gtggccgtgg ccttcgccaa gttcagcgcc gtgttcttcc cggtgctttc gccggacaac
     2401 atcctcctga tcctcggccc ggtgcatgtg agcgccgccc aggtgtacgc cgtgtgcgcc
     2461 atcctgctgc tcacctggtt caatacgcgc ggggtggaac acagcaagac cttcaccacg
     2521 gtgttcaccc tggccaaggt ggccgccgtg ctcgccctgg tggtgggcgg cctcgccgtt
     2581 ggtctgggca ccgatctgct gagcaccaat ttcagccgtg gatgggaggc cgtgcgcaca
     2641 ggttccgatg gcgcactcac gcccctcacc ggcatggcct tgctgagcgc tttgggcgtg
     2701 gccatggtcg gctcgctctt cagcagcgac ggctggaaca gcgtcacctt catcgcggga
     2761 gagatccgcg agccgcagcg caacatcccg ctcagtcttt tcctcggtac tttcatggtg
     2821 accgcgctct acgtgctggc caacatcacc tacctctcat tactgccggt ggaaggcatc
     2881 cagggcgcgg cgttcgaccg cgtaggcacc gccgccgccg aagtgatcat ggggcccgcc
     2941 gccgtgggtg tgatggccgc gctcatcatg gtgagcacgt tcggatgcat caacggttat
     3001 gtgctggtgg gtccgcgcat gtactacgcc atggcccgcg aaggtgtgtt cttcaagaag
     3061 gccgccacct tgaatgcccg gggcgtaccc gcctgggcca cctggctgca atgcgcatgg
     3121 gcctgtctgc tctgtttcag cggcacgtac aacgacctgc tcgattacac caccttcgca
     3181 tcgctcctgt tctatgtggt caccatcggt ggtgtgttca ttctgcggaa gcgcgaaccc
     3241 gacaccccga ggccctatcg cgccttcggt tacccgctgg tgcccgcgat ctacatgctc
     3301 tgtgccctgg ccttctgcgt gaatctgctc gtgatgcagc cgctgttcgc gggcctcggt
     3361 ctgggcatcg tggcgctggg tgccgtggtg tacttcgcta caggtatgca aaggagcggt
     3421 agctgatccg gtgcgggtcg ggcgctgttg ctcatcgtgc atgcggtcca ctcgtacgac
     3481 cgtccggcca tggatccggc cttcgacgaa caccacgcac ccgatcgccc gtcctggata
     3541 ctcagacggt gataa
//
LOCUS       DAOQOC010000160         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12645_length_3554_cov_3.245213, whole genome shotgun sequence.
ACCESSION   DAOQOC010000160 DAOQOC010000000
VERSION     DAOQOC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12645_length_3554_cov_3.245213"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PL106_03630"
     CDS             <1..1001
                     /locus_tag="PL106_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNA32190.1"
                     /translation="ANIVMHTGARPVMVDVLDDFTIDPDAVRRAITQRTKAIIPVDIG
                     GLPARVEDIMRVAQDACGLFTPRLNLRVGGRNPQMLMGRALVLSDAAHSFGARFKDKP
                     LGMQADITGFSFHAVKNLTTAEGGAITFNLPEPFVMDDIYAWFNMMSLHGQSKDALAK
                     SKAGGWRYDVAMAGHKCNMTDLQAAIGLVELERYDRETLPRRKAICDRYHAAFANDDR
                     FLAPVLKDDMRESSYHLFMLRVAKATEDQRDTIIEGITRRGVSVNVHFIPMPMLSFYK
                     GLGYRMEDHPRAYASYSREISLPVYFDLTDQQVDQVIAAVKAAEQEVMGAKDGPRT"
     gene            998..1594
                     /locus_tag="PL106_03635"
     CDS             998..1594
                     /locus_tag="PL106_03635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA32191.1"
                     /translation="MTKRLFDIGFALVLLIVLFPLLLVLALFVAFTSPGGAFFRQTRT
                     GMEGKEFRLLKFRTMRPGSETKGQLTVGTRDPRVTSIGVLLRRSKLDELPQLWNVLVG
                     DMSVVGPRPEVPKYVAMYTLAQREVLRVRPGITGMASIEHIDESDILARSADPERTYV
                     HELMPAKLALDLRYVREHSLALDLRIIAATIMRVFFGR"
     gene            complement(1654..3372)
                     /locus_tag="PL106_03640"
     CDS             complement(1654..3372)
                     /locus_tag="PL106_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32192.1"
                     /translation="MNRLYTSILGIACAVGTQAQVIFNVLEPGNVAGTYNITWVTSGD
                     GWGTIDILDPANSVTDTLALLNDGSGADSLGCDTANVDMSVNGKIAVIYRGSCEFGVK
                     AMGAQTHGARAAIIVNNAAGDPVGMGAGAVGANVTIPVVMISQTDGAILRAAMDAGVV
                     VGFIGSLNGLFANDVGFWKTDVLLPEHATFPTILAANSSEYSVPLAIWLTNYGNQAQT
                     GVTATAEIIQEGNTVYTNTSAPFDLASGDSIMVDLGTFTASSYSGKYTLNYSATIPTA
                     DEFAVNNSWSTTFDVVNELYSFSPIDPQTNLPASPSHGAAGGASAGWQTCIHVQNPNA
                     SRVAVPGFWLSAVTQTNGADLDGELLGISAYHWDTEFGGLSESPEINLVSLMENDFEV
                     PMGYEEGTPVFAQFEEPIVLEDNLRYLFCLTIQSDTIRIGYNSALSYDRNDDQYDQPR
                     NMIYTVSDDTWYTGWSGGSVNTLGIVMIDVNTIGMDENTIGTDALPYPNPASDLIRVP
                     VKDLTGAASLRIINAAGAIASTQRVRASESTVAVNVAGLAPGMYTFEMIPDNGTARTF
                     RVLVGR"
BASE COUNT          667 a         1088 c         1093 g          706 t
ORIGIN      
        1 ccgccaatat cgtgatgcac acgggtgccc ggccggtgat ggtggatgtc ctcgacgact
       61 tcaccatcga tcccgacgcc gtgcgccgtg ccattaccca gcgcaccaag gccatcatcc
      121 ctgttgacat tggtggcctg ccggcacggg tggaggatat catgcgcgtc gcacaggatg
      181 catgcgggct cttcaccccg agactcaacc tgcgcgttgg cggtcggaac ccgcaaatgc
      241 tcatgggccg cgcgctcgtc ctgagcgatg cggcgcattc cttcggcgcg aggttcaagg
      301 acaagccact gggcatgcag gccgacatca cgggcttcag cttccatgcg gtgaagaacc
      361 tcaccaccgc cgaagggggc gccatcacct tcaacctgcc cgaacccttc gtgatggacg
      421 acatctacgc gtggttcaac atgatgagcc tgcacggcca gagcaaggat gcgctggcca
      481 agagcaaggc cggtggatgg cgctacgatg tggccatggc ggggcacaag tgcaacatga
      541 ccgacctgca ggccgccatc gggctggtgg aactggagcg gtacgaccgg gagacccttc
      601 cacgtcgcaa ggcgatctgc gatcgttacc acgcggcctt cgccaacgac gaccgctttc
      661 tggcaccggt gctgaaggac gacatgcgcg aaagcagtta tcacctcttc atgttgcgcg
      721 tggcgaaggc caccgaggat cagcgggata ccatcattga aggcatcacc cggcgcggtg
      781 tgagtgtgaa tgtgcatttt ataccgatgc ccatgctgtc cttttacaag ggcctcggct
      841 accgcatgga ggaccatccg cgtgcgtatg ccagctacag tcgggagatc agcctgccgg
      901 tatatttcga cctcaccgat cagcaggtgg accaggtgat cgcggccgta aaggccgccg
      961 aacaagaggt catgggcgcg aaggatggtc cgcgcacatg accaagcgcc tgttcgacat
     1021 cggtttcgcc ctggtgcttc tcatcgtgct gttccccctt ttgctggtgc tcgcgctatt
     1081 cgtggcgttc acctcgccgg gtggagcctt tttccgccaa acgcgtacag gcatggaagg
     1141 gaaggagttc cggttgctca aattccgtac gatgcgaccg ggaagcgaga ccaagggaca
     1201 actcaccgtt ggtacacgtg atccgcgcgt gacctccatc ggggtcttac tgcgaaggtc
     1261 gaagctggac gaattgccgc aactgtggaa tgtcctggtc ggggacatga gtgtggtggg
     1321 gccgcgtccg gaggtgccga aatacgtggc catgtacacg ttggcgcagc gcgaagtgct
     1381 ccgggtccga cccggcatca cgggcatggc cagcatcgaa cacatcgacg agagcgacat
     1441 cctggcacgc tccgctgatc cggaacgcac ctatgtgcat gagctgatgc ccgccaaact
     1501 cgcactggac ctgcgctacg tgagggagca ctccctcgca ctggacctgc ggatcatcgc
     1561 ggccactatc atgcgcgtct tcttcggcag gtagaaaaac aacacgggcc gcctccgtga
     1621 agaagcgacc cgtgctgccg agtgtgatcc cgatcagcgg cccaccagca cgcggaaggt
     1681 gcgtgcggtc ccgttatcgg ggatcatttc aaaggtgtac atgcccgggg ccagccccgc
     1741 cacgttcacg gccacggtgc tctccgaggc gcgcacacgc tgggtggagg caatcgcgcc
     1801 cgccgcgttg atgatgcgca gcgaagcggc tccggtcagg tctttcaccg gaacgcggat
     1861 gaggtcgctg gccgggttcg ggtacggcaa cgcatccgtc ccgatggtgt tctcgtccat
     1921 gccgatggtg ttcacgtcga tcatgacgat gccaagggtg ttcaccgatc caccggacca
     1981 gccggtgtac cacgtgtcat cggacacggt gtagatcatg ttcctcggtt ggtcgtactg
     2041 atcatcgttg cggtcgtagc tcaacgcact gttgtacccg atccggatcg tgtcgctctg
     2101 gatcgtcagg cagaacaggt agcgcaggtt gtcctccaac acgatcggct cctcgaactg
     2161 cgcgaagacc ggcgtgcctt cctcgtagcc catgggcact tcaaagtcgt tttccatcag
     2221 ggagaccaga ttgatctccg gcgactcgct gagtccaccg aattccgtgt cccagtggta
     2281 ggccgagatc cccagcagtt cgccgtcgag atcggcgcca ttggtctggg tgaccgcgct
     2341 gagccagaag ccgggaaccg ctacgcggct ggcgttcggg ttctgtacgt ggatgcaggt
     2401 ctgccagccc gcactggcac caccggccgc accgtgggaa ggagatgcgg gcaggttggt
     2461 ttgcggatcg atcggggaga agctgtacag ttcattgacc acatcgaagg tggtgctcca
     2521 actgttgttc acagcgaact catcggcggt ggggatggtg gcactgtagt tgagcgtgta
     2581 cttcccgctg tatgagcttg ccgtgaaggt tcccaggtcc accatgatcg aatctccgga
     2641 ggccagatcg aaaggcgcgg acgtgttggt gtacacggtg ttgccttcct gaatgatctc
     2701 ggccgtggct gtaacgcccg tctgggcctg gttgccgtag ttcgtgagcc agatggccag
     2761 cggtacgctg tattcgctgc tattggcggc caggatcgtg gggaacgtgg catgttcggg
     2821 cagcaacaca tcggttttcc agaaaccgac atcgttggcg aacaggccgt tcaagctgcc
     2881 gatgaaacca acgaccacac ccgcatccat ggccgcccgc aggatggcgc catccgtttg
     2941 ggagatcatc accacgggaa tggtgacgtt cgcgcccacc gcacccgcgc ccatgcccac
     3001 tggatcacca gcggcattgt tcacgatgat cgcggcacgt gcaccatggg tttgtgcgcc
     3061 catggccttc acaccgaatt cgcatgagcc ccggtagatc accgcgatct tgccgttcac
     3121 actcatgtcc acgttggccg tgtcgcaacc cagtgagtcg gcaccgctgc catcgttcaa
     3181 caaggcgagc gtgtccgtta ccgagttggc cggatccagg atgtcgatcg tgccccatcc
     3241 gtcgccggaa gtgacccacg tgatgttgta ggtcccggcc acgttccccg gctcgagcac
     3301 gttgaagatc acctgcgctt gcgtgcctac agcgcacgca atgcccagta tggaggtgta
     3361 cagtctgttc atgatggtca ggtcgttttg tgatgagcgg tgcaatgtac acctgtacgg
     3421 ttgatccggc gatgctttcg accaaccatg atcccggtcc gcccaacggc catcggactt
     3481 tgtcctcact tgcctacttt tgtgcatgga aaaaacggtt ccagccccgg cccggcctct
     3541 cccttccacc agtg
//
LOCUS       DAOQOC010000161         3553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12656_length_3553_cov_2.707261, whole genome shotgun sequence.
ACCESSION   DAOQOC010000161 DAOQOC010000000
VERSION     DAOQOC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12656_length_3553_cov_2.707261"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /gene="pbpC"
                     /locus_tag="PL106_03645"
     CDS             complement(<1..1811)
                     /gene="pbpC"
                     /locus_tag="PL106_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNA32193.1"
                     /translation="MRRPGSWLKRHKRKLLVAVVALSVLYSWARWGPMDPLFNTPRST
                     VLLARDGQLLGAATATDGQWRFPGSGPLPPRFVTCLLQFEDRHFYDHCGVRPQSIARA
                     LRQNSRAGRTVSGGSTITMQVARMARGTKDRGYMDKLVEALLALRIETRMDKDSILAL
                     YAHNAPFGGNVVGLDAAAWRWFGRAPDRLSWAESATLAVLPNAPSTVHPGKGRKTLRA
                     KRDRLLARLLAIGAIDRTEWSLATEEPLPDKPLPLPRSAPHLLATLMEHGHAGERIRS
                     TLDPVLQHSLLEVAERYAARLFANEVHNAAALILDVGTGEVRAYMGNLASAGAHYAPQ
                     VDIIRAQRSTGSLLKPFLYADMLGCGELLPDMLTADVPTRYDGLMPRNADERYNGAVP
                     ASEALARSLNVPAVRWLHAHGIARTLTMLRNLGFRTIDRPAEHYGLSLIIGGAESNLW
                     EIAGAYASMARLLKDHGSAGENYRSDEIHAPRVLQDGTDDPTPEGPERPPLSPAAIHF
                     TLKALREVNRPADEQGWRAFADGERIAWKTGTSTGHRDAWAVGLSDRWCVAVWTGNAS
                     GEGRPGLTGPLAAAPLMFDLFGLLGREVPQVRSLWDPP"
     gene            1958..>3553
                     /locus_tag="PL106_03650"
     CDS             1958..>3553
                     /locus_tag="PL106_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32194.1"
                     /translation="MTKYYAAAFMAATFALNTTAQESEGGLPASTRHGLSSADVPVVR
                     TVGIDLAAVTAEDAHRAQESKVPIDARVVPVDADLATSGTWYELPNGDGIWRLRIESP
                     GALATELFFHDFHLPTGGLVYVYSPDGEQMLGGFSAYHNKANGIFATDRITGEACIVE
                     YFEPAEARGEGRFRIATLGHTYRWLGNERADDCEVDVNCSEGANWVPQRDGVVKLRIV
                     DDGGIFFCSGGLVNNVRQDCKRYVLTAMHCGVGVSASDLAQWKFYFGYQRTGCGTGSA
                     TQNRVKTGCVKRADSNDGGGNNGSDFLLLELDDEISASWDPYWLGWDATTNPHIGGVG
                     IHHPAGSEKKISTYSSSAQPNSSWNGMSTHYKVNWVGTANGWGVTEGGSSGSPLFSGE
                     GRVIGTLTGGSSYCNSVQPGGQNQPDFYGRMNYHWTSNGTPANERLKTYLDPDNTGTL
                     VLDGSYNPCGPIGVNEYATLERPLVSPNPATDLVRITFPSDTGAVERVDVLDVTGKLV
                     RSQRPLGGSTVISVEDLRSGVYFV"
BASE COUNT          611 a         1102 c         1237 g          603 t
ORIGIN      
        1 ggcgggtccc acagcgagcg cacttgagga acttcacggc ccaacagacc gaacaggtcg
       61 aacatcagtg gtgcggcggc caggggtccg gtaaggcccg gtcgtccttc gccggaggcg
      121 ttgcccgtcc agaccgccac gcaccagcga tcgctcaggc ccacggccca ggcatcgcgg
      181 tggccggtgc tggtgccggt cttccaggcg atgcgctccc catcggcgaa ggcacgccag
      241 ccttgctcat ccgccgggcg gttcacctcg cgcagggcct tcagggtgaa gtggatggcc
      301 gcggggctca agggcgggcg ttccggacct tcgggagtcg ggtcgtcggt cccatcctgg
      361 agcacccggg gcgcatggat ctcgtccgat cggtagtttt cacctgcgct tccatgatcc
      421 ttcagcaggc gggccatgct ggcgtatgca ccggcgatct cccacaggtt gctttcggca
      481 ccaccgatga tgagcgacaa gccgtagtgt tccgccggac ggtcgatggt gcggaagccc
      541 aggttgcgca gcatggtcag tgtgcgcgcg atgccatgtg cgtggagcca gcgcacggcg
      601 ggcacgttga gcgagcgcgc gagggcttcg ctggcgggaa cggcaccgtt gtaccgttcg
      661 tccgcattgc gtggcatcag tccatcgtac cgggtgggca cgtcggcggt gagcatgtcc
      721 ggcagcagtt cgccgcaacc cagcatgtcc gcgtagagga agggcttcag caggctgccg
      781 gtgctgcgct gcgcacggat gatgtccacc tggggcgcgt agtgcgcacc agctgaggcc
      841 aggttgccca tgtacgcgcg cacctcgccc gttcccacgt ccaggatcag tgcggcggcg
      901 ttgtgtacct cgttggcgaa caggcgggca gcgtaccgct ccgcgacctc cagcaggctg
      961 tgttgcagca cgggatccag ggtgctgcgg atgcgctccc cagcgtgacc gtgctccatc
     1021 aatgtggcca gcaggtgcgg tgccgagcgt ggcaagggca ggggcttgtc gggcaggggc
     1081 tcctcggtgg ccagcgacca ctcggtgcgg tcgatggcgc cgatggccag gaggcgggcc
     1141 agcaaacgat cccgcttggc gcgtagtgtt ttacgtccct tgccggggtg aacggtcgat
     1201 ggcgcgttgg gcagcacggc cagggtggcg ctctcggccc aggagaggcg gtcgggcgct
     1261 cggccgaacc agcgccacgc ggccgcatca agccccacca cgttgccgcc gaagggtgca
     1321 ttgtgcgcgt acagggccag gatggagtcc ttgtccatcc gcgtttcgat gcgcaacgcc
     1381 agcagtgctt cgaccaactt gtccatatat ccacggtcct tggtaccgcg ggccatgcgc
     1441 gccacctgca tggtgatggt gcttccgcca ctcacggtac gcccggcacg gctgttctgg
     1501 cgaagcgccc gagcgatgct ttgcggccgc acaccgcagt ggtcgtagaa gtgccggtcc
     1561 tcgaattgca gcaaacaggt gacgaagcgc ggtgggagcg ggccgctgcc ggggaatcgc
     1621 cattgcccat ccgtggcggt cgcggcaccg agcaactgcc cgtcgcgggc caggagcacg
     1681 gtggatcggg gcgtgttgaa gagcggatcc atcggccccc atcgggccca gctgtacagg
     1741 accgaaaggg cgaccaccgc caccaacagc ttgcgtttgt ggcgcttcag ccaggaaccc
     1801 ggtcggcgca tgcgcttcaa aggaacgggt acgcgcgccc cgtgttgttc ccgggcggca
     1861 tgggcatgct tccgtgccgt gttggtccgc agggatacgc ggttggcctt ggggtgcgta
     1921 gtttggcggt ccctaacgtt ggatcacatt cccccacatg acgaagtact acgcagcggc
     1981 attcatggcc gcaacattcg cactgaacac aacggcccag gaaagtgagg gcggactgcc
     2041 tgcgtccacg cggcatgggc tgagctcggc cgatgtaccc gtggtgcgca cggtcggcat
     2101 cgacctggcc gcggttaccg cggaagatgc acaccgggcc caggaaagca aggtacccat
     2161 cgatgcccgc gtggtgccgg tggatgcgga cctggccacg agtggaacat ggtacgagtt
     2221 gcccaatggc gacggcatct ggcgtttgcg gatcgaaagc ccgggtgcgc tggccacgga
     2281 gttgttcttc cacgatttcc acctgcccac cggcggcctg gtgtacgtgt acagtcccga
     2341 tggcgagcag atgcttggcg ggttctcggc ctaccacaac aaagcgaacg ggatcttcgc
     2401 cacggaccgc atcaccggtg aggcgtgcat cgtggagtac ttcgaaccgg ccgaagcccg
     2461 gggcgagggg cgcttccgca tcgcaacact gggccatacg taccgctggc tgggcaacga
     2521 acgggccgac gattgcgagg tggacgtgaa ctgttctgaa ggcgccaatt gggtgccaca
     2581 gcgggacggc gtggtgaagc tgcgcatcgt ggacgatggc gggatctttt tctgctcggg
     2641 cggcctggtg aacaacgtgc ggcaggactg caagcgctat gtgctcaccg ccatgcactg
     2701 cggggtcggc gtgtcggcat cggacctggc ccagtggaaa ttctacttcg gctaccagcg
     2761 cacgggctgc ggcaccggct cagccacgca gaaccgtgtc aagaccggct gtgtgaaacg
     2821 cgccgacagc aacgatgggg gcggcaacaa cggatcggat ttcctgcttc tggaactgga
     2881 tgatgagatc agcgcctctt gggacccgta ctggctgggt tgggatgcca ccacgaaccc
     2941 ccacatcggc ggagtcggca tacaccatcc ggccggcagc gagaagaaga tcagcacgta
     3001 cagcagtagc gcgcagccca acagctcgtg gaacgggatg agcacgcact acaaggtgaa
     3061 ctgggtgggc acggccaacg gctggggcgt taccgagggc ggctcttccg gctcgccgtt
     3121 attcagcggc gaaggacggg tgatcggaac gctcacgggt gggtcgtcct attgcaacag
     3181 tgtgcagccc ggcgggcaga accagccgga tttttacggc cgcatgaatt accattggac
     3241 cagcaacggc actccggcca acgagcgctt gaagacctac ctggatccgg ataataccgg
     3301 tacgctggtg ctcgatggca gctacaaccc gtgcggcccc atcggggtga acgagtacgc
     3361 cacgctcgaa cggccgctgg tatcgcccaa cccggccacc gaccttgtgc gcatcacctt
     3421 cccgagcgat actggcgcag tggagcgggt ggatgtgctg gacgttaccg gcaagctggt
     3481 gcgttcgcag cgcccgctgg gcggaagcac ggtgatcagt gtggaagacc tgcgttcagg
     3541 tgtgtacttc gtg
//
LOCUS       DAOQOC010000162         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12799_length_3532_cov_3.610871, whole genome shotgun sequence.
ACCESSION   DAOQOC010000162 DAOQOC010000000
VERSION     DAOQOC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12799_length_3532_cov_3.610871"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..322
                     /locus_tag="PL106_03655"
     CDS             134..322
                     /locus_tag="PL106_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32195.1"
                     /translation="MKHFALVMSVLYVLAGGLLLATDILHDRIGVLRVPLGITLVIYG
                     TVRAYLWRRKYADGSGDA"
     gene            319..1263
                     /locus_tag="PL106_03660"
     CDS             319..1263
                     /locus_tag="PL106_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32196.1"
                     /translation="MKYACSIPLLALLLVTACGDPPDPRTDDVPTKGHVLILADEDLR
                     ELVEAERFMFGSIYRKAILDIRYLPEASLLKDMLNDSVRCSILTTRPGGDQERYFNGR
                     NVKAHVIPIATDGIAVLVNKRVKLNGLSTGAVVELLSTPKAFAYKGWELPEGEGRLDA
                     PRAWFISSGSGVARSLVDSLFGTSGQQRILAMTADSLPELIVRIEQDPQVVAFVSYSS
                     ISDLDDPHVRALRERVKLLPISATDTSQAILPSQSTLADGSYPLRRPVFMLLAEGKSG
                     LGTGFVSFVANHKGQRIILKHGLAPNKVPPREIEVVAQ"
     gene            1278..2969
                     /locus_tag="PL106_03665"
     CDS             1278..2969
                     /locus_tag="PL106_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32197.1"
                     /translation="MKKFVLRASKSSVRSLSVVLLGATTLSAGAQTLADAIKLTDKEQ
                     FDKATLAFRKVIASEPQNGEAWFYFGENYWENQQADSAEACYRQGNGVGGKFPLNKVG
                     LGKVLWAQGKKAEAQTMFDQAIADATDKANKIPKPQQANTYREIAEAMAQGARPDLIK
                     AQELIARSLELDAKNPETYVLKGDVLFDLNPRDGSAPLENYKIAINMDALNAKPVARK
                     AFMYYRAKNNPAAIEEYTRAIGIDGSFAPAYSGRAEAYVKTGDFVKAEADMAHYLELN
                     TGNQSARVRYAQFLFLVGKYQESLGLINTLEQEGVKNIVLKRLQGYDQVEMKDSVNAK
                     STLEAYFAEQPEDKRIAADYEYYGKSLLLVHQDSLAAENMLKGARMDRTKDHLYTEAA
                     KTFVRARAYERAVAAMQERIAAGKPETNDYYYLGDAALKAGMYQTADSAWATYTQRNP
                     NAYQGYRYRAQTQAATDTLAIGDRTWSAKPYYDEMLLKMKPEEREKSKSHYEEALNYM
                     GLYYLYNKEARDLPKAKCYFEQVKALNAGTSITKQVNEVMLMTKELKDVTPAGCE"
     gene            3101..>3532
                     /locus_tag="PL106_03670"
     CDS             3101..>3532
                     /locus_tag="PL106_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNA32198.1"
                     /translation="MLSKTNRLTLWILIAMVVGIGVGYAMHLGMDVAVRKGVAENLKI
                     LTDVFLRLVKMIIAPLVFSTLVVGIAKLGDMRTVGRVGGRALLWFISASLVSLLLGML
                     LVNLLKPGVGLHLEASSDVSDVVEKTGVFTLRDFIAHLFPSS"
BASE COUNT          740 a         1046 c         1125 g          621 t
ORIGIN      
        1 tcaacgaggc ggccgaacgg gtgatccgga tcatgcccag gtggaaaccc gcgcgttaca
       61 aggaccgggc ggtaccttgc gtgatggtgc tccccattga ataccgcacg ggatggtagc
      121 ttcgtccccg gccatgaagc actttgccct ggtgatgagt gtgctgtacg tgctggcggg
      181 tggcctgctg ctggccacgg acatcctgca cgaccggatc ggtgtgctcc gcgtaccgtt
      241 gggcatcacg ctggtgatct acggcacggt gcgcgcctat ctctggcggc gcaagtacgc
      301 ggacggttcc ggggacgcgt gaagtacgct tgttccatcc cgctgctggc gttgctgttg
      361 gtcacggcat gcggcgatcc gcccgatccc cgcaccgatg acgtgcccac caagggccat
      421 gtgctgatcc ttgccgacga ggacctgcgc gagctggtgg aggccgaacg cttcatgttc
      481 gggagcatct acaggaaggc catacttgat atcaggtacc tgccggaggc cagcttgttg
      541 aaggacatgc tgaacgacag tgtgcgttgc agcattctca ccacacggcc cggcggcgac
      601 caggagcgtt acttcaacgg gcgcaacgtg aaagcgcacg tcatacccat cgccacggac
      661 ggcatcgcgg tgctggtgaa caagcgcgtg aagctgaacg gcctgagcac cggtgcggtg
      721 gtggaattgc tctccacacc gaaggcattc gcgtacaaag gctgggagtt gcccgagggg
      781 gaagggcgac tggacgcgcc acgcgcctgg ttcatctctt ctggcagcgg cgtggcacgc
      841 agcctggtgg attccctgtt cggcaccagt ggccagcaac ggatcctggc catgaccgcg
      901 gacagtctgc cggagctgat cgtgcggatc gagcaggacc cgcaggtcgt cgctttcgtg
      961 tcgtattcgt ccatcagcga tctggatgat ccgcacgtga gggcgctgcg cgagcgggtg
     1021 aagctcctgc cgatcagcgc taccgacacc tcgcaggcca ttctgcccag ccaaagcacg
     1081 ctggccgacg ggtcctaccc gctgcggagg cccgttttca tgcttctggc cgagggtaaa
     1141 agcggtcttg gcaccgggtt tgtttccttt gtggccaacc ataaagggca acgcatcatc
     1201 ctcaagcacg gtctcgcgcc caacaaggtc cctccccgcg agatcgaggt ggtagcccaa
     1261 tagcagaaca acggaagatg aagaagttcg tgcttcgtgc gtcgaagagt tcagtgagaa
     1321 gtctgtcggt tgtgctgctg ggcgcaacca ccctgagtgc cggtgctcaa acccttgcgg
     1381 acgccatcaa gctcaccgac aaggaacagt tcgacaaggc gacactggcg ttcaggaaag
     1441 tgatcgccag cgagccccag aacggggagg catggttcta tttcggcgag aactactggg
     1501 agaaccagca ggcggacagt gctgaggcgt gctaccgtca gggcaacggg gtgggcggca
     1561 agttcccgtt gaacaaggtg ggtttgggca aagtgttgtg ggcccaaggc aagaaggccg
     1621 aggcgcagac catgttcgac caggcgatag ccgatgcgac cgacaaggcg aacaagatcc
     1681 ccaagccgca gcaggccaac acctaccggg agatcgccga agccatggcc caaggcgccc
     1741 gacctgacct gatcaaggca caggaactga tcgcccgatc gttggagctg gacgcgaaga
     1801 atcccgagac gtatgtactg aagggtgacg tgctgttcga cctcaacccg cgcgatggtt
     1861 cggccccgct ggagaactac aagatcgcca tcaacatgga cgcgctcaac gccaagcccg
     1921 tggcgcgcaa ggccttcatg tactaccgcg ccaagaacaa cccggccgcc atcgaggagt
     1981 acacccgggc catcggcatc gatggatcgt tcgcgccggc ctattcgggc cgtgcggaag
     2041 cgtacgtgaa gaccggcgat ttcgttaagg cagaagccga catggcacac tacctggaac
     2101 tgaacaccgg caaccagagt gcccgcgtgc gctacgccca gttcctcttc cttgttggca
     2161 agtaccagga gtcgctcggc ttgatcaaca ccttggaaca ggaaggcgtg aagaacatcg
     2221 tgctcaagcg cctccagggc tacgaccagg tggagatgaa agactcggtc aacgcgaagt
     2281 ccacattgga ggcctacttc gccgagcagc cggaggacaa gcgcattgcg gcagactacg
     2341 aatactacgg taagagcctg ctcttggtgc atcaggattc gcttgcggcc gagaacatgc
     2401 tcaagggggc gcgcatggac cggacgaagg accacttgta caccgaggcg gccaagacct
     2461 tcgtgcgggc cagggcctac gagcgtgcgg tggccgccat gcaggagcgc atcgctgcgg
     2521 ggaagcccga aacgaacgac tattactact tgggcgatgc ggcgttgaag gccgggatgt
     2581 accagaccgc ggacagcgca tgggccacct acacccagcg caaccccaac gcgtatcaag
     2641 gttatcgcta ccgcgcccaa acccaggccg ctaccgatac gctggccatc ggggaccgca
     2701 cctggtcggc caagccgtac tacgacgaga tgctcctgaa aatgaaaccg gaggagaggg
     2761 agaagagcaa gtcgcactac gaggaagcgc tcaactacat gggcctgtac tacctgtaca
     2821 acaaggaggc ccgcgacctg cccaaggcca agtgctactt cgagcaggtg aaggcgctca
     2881 acgccggcac cagcatcacc aagcaggtga atgaggtgat gctgatgacc aaggaactga
     2941 aggacgtgac accggccgga tgcgagtagt gtacggttgt tgataggacc gctcgtacgg
     3001 atccaacgaa caaggggccg gtactgccga ccccttgttc gttcagggca tgatggcggc
     3061 cctggcaacg tgctgaaccc tgacgcctac cttgcccccc atgttgtcca agaccaaccg
     3121 cctcacgctt tggatcctca tcgccatggt cgtcggcatc ggtgtgggct atgccatgca
     3181 cttgggaatg gatgtggccg tgcgcaaggg tgttgcggaa aatctgaaga tcctcaccga
     3241 cgtcttcttg cgactggtga aaatgatcat cgcaccgctg gtgttcagta ccctggtggt
     3301 gggcatcgcc aagctggggg acatgcgaac ggtggggcgc gtaggtggcc gtgccctgct
     3361 gtggttcatc agcgcatcgc tcgtttccct gctgctgggc atgttgttgg tgaatctgct
     3421 caagcccggc gtgggactgc acttggaagc cagtagcgat gtatcggacg tggtggagaa
     3481 gaccggggtg ttcaccttgc gcgacttcat cgcccacctc ttccccagca gc
//
LOCUS       DAOQOC010000163         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12811_length_3530_cov_3.858705, whole genome shotgun sequence.
ACCESSION   DAOQOC010000163 DAOQOC010000000
VERSION     DAOQOC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12811_length_3530_cov_3.858705"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PL106_03675"
     CDS             <1..1671
                     /locus_tag="PL106_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32199.1"
                     /translation="AWTVDGTDGAIWHTGTTAPLGAYSPANERIQSTTFSNGFAKFAG
                     DSANCTWSDNTPTAIPSDQFTNWDGSLVSPVIDLSATPAAILTFQQRARWCCGDMPDM
                     VGVSTDNGATWVDVPAYDNLAINTLSATVTKSLSITGLIAANPANVQIRFHHDGANYQ
                     TSHYHWQVDDISISTAPATDARVFDGYCSHRGDGTEHGRIPQNQLGGTMNLGGYMVNE
                     GATPLTNATLTAVVTDNATSTTQFTASATSPSLVPGDTLIMNEWPTFGTLATGAYTVT
                     FTGTSDEQAAEADPDNNGWVRTFQVTESGSGMIYSLDEIGGHPQGTQVLSSIGTDDFT
                     DGADGLIVFTYYPVQEDLTVDAISFRLATGSTSGGFYSVAIHDSTAILNDDPGSPLAE
                     SDEFDLTVSTGTVVVPLTEPITLGPGAYYASVTMYSNANENDFKISDDATIPQPGWSS
                     GIFVQGASYTNGTALAIQLRNAAVGMDENSLEGVGIWPNPTDGVLNVNVRETGTYRVD
                     VMNSLGQTVSTQRMNGSSKVDLGGLAKGVYSVRVSTDKASTTQSVVLR"
     gene            complement(1737..2495)
                     /locus_tag="PL106_03680"
     CDS             complement(1737..2495)
                     /locus_tag="PL106_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA32200.1"
                     /translation="MIEVRGLSKRFGRTQVLTDINAVFQKGKVNQVIGKSGSGKSVLC
                     KCIVGLFEPEEGEVLYDGRSFHNLELEQKRQVRQEIGMLFQSSALFDSLTVLENVLFP
                     LRMFGTMDEAGMLDRAHLCLKRVNIEGKDRLYPSEISGGMQKRVGIARAIAMNPRYLF
                     VDEPNSGLDPQTSILIDNLIDELTAEYDMTTIMITHDMNSVMEAGDNILFIHEGRAWW
                     KGSREELLRSDNKELNDFIFASGLMRKVKDAMKR"
     gene            complement(2492..3232)
                     /locus_tag="PL106_03685"
     CDS             complement(2492..3232)
                     /locus_tag="PL106_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32201.1"
                     /translation="MDLLFHIGRYFQLIRETFTRPERFGLYWKRTLEEVDLLGVKSLG
                     IVALLSVFMGAVITIQTKTNIDSPLIPLYVVGFTARQSTILEFSPTIISLILAGKVGS
                     TIAAQIGSMRVREQIDALDIMGVNSAGYLILPKVIACVVINPFLVVISMFLSIFGGYL
                     AGTLSGLVSGEVYLQGIQTDFTPYHVTYALIKTVVFAFIITTVSAYQGYYTQGGTREV
                     GISSTRAVVYSDVVILIMNYLLTQLLLI"
     gene            3328..>3530
                     /locus_tag="PL106_03690"
     CDS             3328..>3530
                     /locus_tag="PL106_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32202.1"
                     /translation="MNTRPTGGCDMNRQRSITTMILAGALLASHLRHLGYHVTVEILR
                     PNEEWGELEDRQREREVVLPLADL"
BASE COUNT          707 a         1170 c         1023 g          630 t
ORIGIN      
        1 gcatggaccg tcgacgggac cgatggtgcc atctggcaca ccggcacaac ggcgccactt
       61 ggtgcctatt caccggccaa tgagcgcatc caatccacca ccttcagcaa cggcttcgcc
      121 aagttcgccg gcgattcggc caactgcacg tggagcgaca acacgcccac cgcaattccc
      181 tcagaccagt tcaccaactg ggacggcagc ttggtctccc ctgtgatcga cctgagtgct
      241 accccggccg ccattcttac tttccagcaa cgcgcacgct ggtgctgcgg cgacatgcct
      301 gatatggtcg gggtgagcac cgacaatggc gcgacctggg tcgatgtacc cgcatacgat
      361 aacctggcca tcaataccct ttcggccacg gtgaccaaga gcttgagcat cactggcctg
      421 atcgcggcca accccgccaa cgtgcagatc cgcttccacc acgatggggc caactaccag
      481 acctcacact accactggca ggtcgacgat atcagcatca gcacggcacc ggccaccgac
      541 gctcgtgtgt tcgacggcta ctgcagccat cgcggcgacg gcaccgagca cggccgcatc
      601 ccgcagaacc agcttggagg taccatgaac ctcggcggct atatggtgaa cgaaggtgcc
      661 acgccactga ccaatgccac gcttaccgca gtggtcaccg acaatgcgac cagtaccacc
      721 cagttcaccg cttcagcaac gtcgccctcc ctggtccctg gcgatacgtt gatcatgaac
      781 gaatggccca ccttcggtac cctcgccacg ggggcgtaca cggtcacctt caccggcacc
      841 tccgatgagc aggccgccga ggcagatccc gacaacaacg gctgggtgcg cacattccaa
      901 gtgaccgaat cggggagtgg gatgatctac tccctggacg agatcggtgg ccacccgcaa
      961 ggaacccagg tcctcagcag catcggcacg gatgacttca ccgatggcgc cgatgggctc
     1021 atcgtgttca cctactatcc cgtacaggag gacctgacgg tggacgccat ctcgttccgc
     1081 ctggccaccg gctccaccag cggcggcttc tacagcgtgg ccatccacga cagcaccgcc
     1141 atcctgaacg acgatccggg ctcgcccctt gccgagagcg atgagttcga cctgaccgtt
     1201 tccacgggca ccgtggtggt acccctgacc gagcccatca cgctgggccc cggtgcgtac
     1261 tatgccagcg tgaccatgta cagcaatgcc aacgagaacg acttcaaaat ctccgacgac
     1321 gccaccatcc cgcaaccagg ctggtccagc gggatcttcg tgcagggcgc ttcctacacg
     1381 aacggtacgg ctctggcgat ccaactgcgc aacgcggccg tgggcatgga cgagaacagc
     1441 ctggaaggtg tgggcatctg gcccaatccc accgatggcg tgctcaacgt gaacgtgcgc
     1501 gaaaccggca cctaccgcgt ggatgtgatg aacagcttgg gccagacggt gagcacccag
     1561 cgcatgaacg gcagctccaa ggtggacctg ggcggcctcg cgaagggtgt gtacagcgtg
     1621 cgcgtttcca cggacaaagc ctccaccacg cagagcgttg tgctgcgctg agcatcggaa
     1681 ctacgaagtt gaaggggctg tctcgaaaga ggcggcccct tccgcttttc cggggatcag
     1741 cgcttcatcg cgtccttcac cttgcgcatc aggccgctgg cgaagatgaa gtcgttcaac
     1801 tccttgttgt cgctccgcag caactcctcg cgcgaccctt tccaccaggc acgcccctcg
     1861 tggatgaaga ggatgttgtc gcccgcctcc atcacgctgt tcatgtcgtg cgtgatcatg
     1921 atggtggtca tgtcgtactc ggccgtcaac tcgtcgatca ggttgtcgat caggatgctc
     1981 gtctgtggat ccagcccgct gttcggctcg tccacaaaaa gataccgtgg gttcatggcg
     2041 atggcccgtg cgatacccac acgtttctgc atgccaccgc tgatctcact ggggtagagc
     2101 cggtccttgc cttcgatgtt cacccgcttg aggcacaggt gcgcgcgatc cagcatcccc
     2161 gcctcatcca tggtgccgaa catgcgcagt ggaaagagca cgttctccaa caccgtgagc
     2221 gaatcgaaca gggcgctgct ctggaacagc atgccgatct cctggcgcac ctggcgcttc
     2281 tgctccagtt ccaggttgtg gaacgagcga ccatcgtaga gcacctcgcc ctcctccggc
     2341 tcgaacagcc ccacgatgca cttgcacagc acactcttgc cgctgccgct cttgccgatc
     2401 acctggttca ccttgccctt ctggaacacc gcgttgatgt ccgtgagcac ctgcgtgcgc
     2461 ccgaaccgct tgctcagccc gcgcacctcg atcatatcag caggagttgg gtgagcaggt
     2521 agttcatgat caggatcacc acgtcgctgt ataccacggc acgggtactg ctgatgccca
     2581 cctcgcgcgt gcccccctgg gtgtagtagc cctggtacgc gctcaccgtg gtgatgatga
     2641 aggcgaagac cacggtcttg atcaatgcgt aggtgacgtg gtagggcgta aaatccgtct
     2701 ggatgccctg caggtagacc tcgccgctca ccagacccga gagcgtgccg gccaggtagc
     2761 ccccgaagat gctcaggaac atgctgatga ccaccaggaa ggggttgatc accacgcagg
     2821 cgatgacctt gggcagaatg aggtagccgg ccgagttcac ccccataatg tccagcgcat
     2881 cgatctgctc gcgcacccgc atggaaccga tctgcgccgc gatggtggag cccaccttgc
     2941 ccgccaggat caggctgatg atggtggggc tgaactccag gatggtgctc tgccgcgccg
     3001 tgaatcccac cacgtacagg gggatgagcg ggctgtcgat gttggttttg gtctggatgg
     3061 tgatgaccgc ccccatgaag acgctgagca gggcgacaat gcccaagctc ttgaccccca
     3121 ggaggtcaac ctcctccagg gtgcgcttcc aatacaggcc gaaacgctcg ggccgggtga
     3181 acgtctcccg gatgagttgg aagtagcggc cgatatggaa cagcagatcc accgatggaa
     3241 gggtgccaag ctacaggccc caccgggtcg tccgccacgg tcggaaaaag ggcttccaca
     3301 accggatgtg ggtcgttaca tttggccatg aacacccgcc cgaccggcgg gtgcgacatg
     3361 aacaggcaac gctccatcac caccatgatc ctcgccggcg cgttgctcgc gtcgcatcta
     3421 cggcacctgg gctaccacgt caccgtggaa atcctgcggc cgaacgagga atggggcgaa
     3481 ctcgaagacc ggcagcgaga acgcgaggtc gtgctgccac tggcggatct
//
LOCUS       DAOQOC010000164         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12841_length_3525_cov_4.407493, whole genome shotgun sequence.
ACCESSION   DAOQOC010000164 DAOQOC010000000
VERSION     DAOQOC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12841_length_3525_cov_4.407493"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PL106_03695"
     CDS             <1..364
                     /locus_tag="PL106_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32203.1"
                     /translation="ARNLLERFLSDSLLEPVILPGTERTMIASTTSKAGQQSRIEARP
                     NPASTLVSVLLRGAPIGQGAYLNLADAKGMVVKRIPLIPFQSFVDLNVADMSSGLYRL
                     SLSGINCEYPGVSLSIVH"
     gene            897..1838
                     /locus_tag="PL106_03700"
     CDS             897..1838
                     /locus_tag="PL106_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32204.1"
                     /translation="MGSVAWIREYGTSVRDEGYSLDTTPDGGFILGGHTWLSATNWQG
                     YLVKTDSAGNALWHRYLGGPYKDGWANVVCTADSEYVSVGSYATYQTDNTTNKRLYAA
                     RLNADGDVEWERQYAMASEVNELSSVTELADGTFIAPGIYYDTTSYKGVLLRFDQNGD
                     SIWMRTYQHPPLVGVFSVHWLAHAIQTTDGGFVATGSCNDGEQDLWVLKVDSFGCLVP
                     GCQLYDNIAEQGLELNVLAYPNPTRGKLFLSFRSAHQPTGEFILFNSAGADVQRFAPG
                     GSSVEIDYDIGHHPAGMYLLQYQEQGRVKWSQKVIKE"
     gene            1835..3502
                     /locus_tag="PL106_03705"
     CDS             1835..3502
                     /locus_tag="PL106_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32205.1"
                     /translation="MKHTIALPRNTSSEGRLGAVTRIRANAPGVQHRIGLSVISMLFS
                     ALLLAQDLRPLWATSIPASHVNVIADSSYGCWLSGGYGQPLVFGPDTLLVEGGGANGF
                     VARYDGFGSAQWGLRTNAGAFHMMARSGGAGIIGAFSGTFEIGDSTATSAQGLGLAIT
                     LIDDDGTIASVHPIPDVLRVSGNFSLHDMAPFMDGSSDVGLYGSFGDTLMVADTSLVG
                     VDGTHFLARIGDDGALRWARSIGIAGQTGQLVIDGSGRSIITGENPSDQLPDTILSFG
                     GVFVASFGADGSFLWSQVFGDQPTSYGPMVDVDQQDRVIFGYTNAAFDFGTSSVPEVH
                     CFSAEGTALWSATAEGATPHLFSGTIRTRPSGPIVLTGWGYGPSVNFGTHLFTPGNDP
                     TSFIATLSASGEWVDLYQDAGDHNVWGASLTEEVGGKMYGVGRFELTIDLSPWVPPVV
                     SALPEATYVIELGPSAMPVPEAELFPVRLWPNPTNDRIFLRFPDAVEPNGEFVLLNGA
                     AQAVQRFRPGCKQVEIDLDIGHRVSGLYMLQYRDADGVRWSGRIVKE"
BASE COUNT          687 a          938 c         1083 g          817 t
ORIGIN      
        1 agcgcggaac ctgctcgagc gcttcttgtc tgattctttg ctggaacctg tgatcctgcc
       61 tggtacggaa cggactatga tcgcctctac caccagcaaa gccgggcagc agagccgcat
      121 cgaggcgcgt cccaatcctg ccagcacgct cgtcagtgtc ctactgcgtg gtgcgcccat
      181 cgggcagggg gcttacctaa acctcgcgga tgctaaagga atggtggtca agcgtattcc
      241 gcttattccg ttccagtcgt tcgtggatct gaatgtcgca gacatgtcca gtggcctcta
      301 tcggttgtcc ctgtcaggaa tcaactgtga gtaccccggt gtgtcgttga gcatcgtcca
      361 ctgacatggc tctccattct tgggttgctg cctttgcagt tctctgggcg tgtactgccc
      421 tggctcaacc cttcagtgtg cgccatgact acgcccagtc atctgatact gactttgggg
      481 tccgtgcgtt cttagttggt acttcattta ttgtcgtgca aagagggcac atttcaccag
      541 gaggtgtaaa cagactttgc ttcaccgcaa taagcggcaa tggaacagca accgacgtgg
      601 agtctttcgg tagggatagc ttaggctgta ctgcgggtgg ctgcaatggt ccaacttctg
      661 ctttaggcgg agatatcttc atctgtggct cgacagatga tggcacccgt gtagcggctc
      721 tcacttggag attgagttcg acacatgata gtctttggtc ggtctttttg ttccctgaca
      781 ccaccgacaa ctcgctcgga aagatggtgc ggtgtcgaac tggaatgaac tattcgacag
      841 gcggggtttg gcatgggtcg gcaagtgggc aggtgttttt ggccaagttg gatagcatgg
      901 gctccgttgc gtggatacgg gagtacggaa catcagtccg agatgaaggc tattcactgg
      961 ataccacacc tgatggcggc ttcatacttg gcggtcacac ttggctcagc gccacgaact
     1021 ggcaaggcta tctggtgaag accgactctg ctggcaacgc cctatggcat cggtatctgg
     1081 gtggaccgta caaggatggt tgggccaacg tggtatgcac ggcggatagc gagtacgttt
     1141 cggtcggcag ctacgcgacc tatcaaaccg acaacaccac gaacaagcgg ctctatgccg
     1201 cacggctgaa tgcggacggt gatgtggaat gggagcgaca gtacgctatg gccagcgaag
     1261 tgaacgagct ctcctccgtg accgaactgg cggacgggac tttcatagcg ccaggcattt
     1321 actatgacac cacttcgtac aagggtgtgc tcttgcgttt tgaccagaac ggggatagca
     1381 tctggatgcg cacgtaccaa catccaccac tggtgggtgt attcagcgtc cactggctgg
     1441 cccatgctat ccaaacaacg gatggagggt tcgtggcgac cggttcgtgc aacgatggag
     1501 aacaggacct gtgggtattg aaggtggaca gcttcggctg tctggtgccg ggctgccagt
     1561 tatatgacaa catcgccgag cagggcttgg aactcaacgt gctggcttat cccaatccaa
     1621 ctcggggcaa gttgttcctg agtttccgat cggcgcatca gcccaccgga gagttcatcc
     1681 tgttcaattc cgcaggcgcg gatgttcagc gctttgcacc tggtggttcg agtgtggaga
     1741 tcgactacga cattggtcat catccagcag gcatgtatct gttgcagtac caagagcagg
     1801 gtcgggtcaa gtggtcacaa aaggtcatca aggaatgaag cacacgatag ccttgccccg
     1861 taacacgtca agcgaaggac gcttgggagc agtgaccagg atccgagcga atgccccggg
     1921 tgttcaacac cgcatcggct tatcggtgat cagcatgctt ttcagtgcgt tgctgctcgc
     1981 tcaagacctc cgccccctgt gggccacatc gatacccgcc tcgcatgtga acgtgatcgc
     2041 cgacagttct tatggttgtt ggctcagcgg gggctatggg cagccgctgg tgttcggccc
     2101 ggacacgctg ttggtcgaag gaggtggtgc gaacggattc gtggcgcggt acgatgggtt
     2161 cggatcggcg caatggggcc tacggacgaa cgcgggcgcc tttcatatga tggcccgctc
     2221 gggtggcgca ggcatcatcg gtgctttcag cggaacgttc gagatcggcg attccacggc
     2281 gacctcggca cagggtctgg gattggccat tacgctgatc gacgatgatg gcaccatcgc
     2341 gtcggtccat ccgatcccgg atgtgctgcg ggttagcgga aatttcagcc tccatgatat
     2401 ggcaccgttc atggatggct cgagcgatgt cggattgtat ggttccttcg gggatacgtt
     2461 gatggtcgcc gacaccagtt tggtcggggt tgatgggacc cacttcctgg cccgcatcgg
     2521 tgacgatggc gcgttgagat gggcgcgatc gatcggaatc gctggtcaaa ccgggcagct
     2581 ggttattgat gggagcgggc gttcgatcat tactggagag aacccgtcgg atcaattgcc
     2641 cgatacgatc ctctcattcg gcggggtctt cgtcgcctcc tttggcgctg atggatcttt
     2701 cttgtggagc caggtgttcg gtgaccagcc cacgagctat ggtcccatgg tcgatgtgga
     2761 ccagcaagac cgcgtgatct tcggttacac taatgcggca ttcgacttcg gtacatcgag
     2821 cgttccggag gtccattgtt tcagtgccga aggcacagcc ctgtggagtg cgacggccga
     2881 gggggcaacc ccccatctgt tctccggaac gatccgaacc aggccctcgg gaccgatcgt
     2941 gcttacaggg tggggatacg gtccatcggt caatttcggg acccatctgt tcacgccggg
     3001 caacgacccc acgagcttta tcgccacgct atccgcaagc ggcgagtggg tggacctgta
     3061 ccaggacgcg ggcgaccaca acgtttgggg cgcttccttg actgaagagg tggggggaaa
     3121 gatgtacggc gttgggcgtt ttgagttgac catcgacctc agcccgtggg ttccgcctgt
     3181 ggtatcagcc ttaccggaag caacgtacgt tatcgagctt gggccctcag ccatgcctgt
     3241 cccagaggct gaactatttc ctgttcggct gtggcccaat cccaccaacg acagaatttt
     3301 cctccgcttt cccgatgcag tggaaccgaa tggcgagttc gtgttattga atggcgcagc
     3361 acaggcagta cagcgtttcc ggcccggctg caaacaggtt gaaattgacc tggacatcgg
     3421 gcatcgtgtg tcgggtctgt acatgctcca gtaccgtgat gcggatgggg ttcgatggag
     3481 cggccgcatt gtcaaggagt agttccacac tttgtgcgca ccttt
//
LOCUS       DAOQOC010000165         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_12914_length_3515_cov_3.074855, whole genome shotgun sequence.
ACCESSION   DAOQOC010000165 DAOQOC010000000
VERSION     DAOQOC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12914_length_3515_cov_3.074855"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PL106_03710"
     CDS             complement(<1..523)
                     /locus_tag="PL106_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32206.1"
                     /translation="MEDRVITGELVKLVQDMAHRFGEERSRLLEARGQRRELFRQGQV
                     ERLEETAHIRQAVWKVDPVPAELLERRVELLGGCSKQEIIEGMNCGAKSYIADLWGLT
                     LGDPASILKAHRNLERLVDGQLHYVDGHGERVRVNPASTTRILFAPRPLLATEPSVRD
                     QGAPVPASFFDLAV"
     gene            661..1980
                     /locus_tag="PL106_03715"
     CDS             661..1980
                     /locus_tag="PL106_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA32207.1"
                     /translation="MQRELLTSVLTMAVLTAGAQARKIAFSEFDLPNGLHVILHEDHG
                     TPIVAVSVMYHVGSKDEASNRRGFAHFFEHLLFEGSDNIARGEFSRYVEKAGGVLNAN
                     TNGDRTYYYEVLPSNQLELGLWLESERMMHARVDQIGVDTQREVVKEERRQRYENQPY
                     GSLLPEVLKRAYTEHPYQWPTIGFMEDLNAASEADYKTFYKTFYVPNNAVLVVAGDID
                     PQGTRALVEKYFAAIPKGDKIPRNTVVEPPLGTELRDVVHDRIQLPAVVLAYRIPGYG
                     TKDFYAVDMLNRLLSNGNSSRLNKRVKDQEQKALYVGAFSFPLEDPGLAIAFAIANGG
                     VSPDSLEHAMDEVFAEVREKGVPAAELEKLKAGLETEFVNANARVAGIAENLASAYTF
                     LGSTAKVNTELDQYMHVTTDDLQRAAQHYFDPKKRVVLHYLPMVGDK"
     gene            2001..>3515
                     /locus_tag="PL106_03720"
     CDS             2001..>3515
                     /locus_tag="PL106_03720"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA32208.1"
                     /translation="MTFHALRALAAVSVLSMSISMTAQIDRTRAPKPGPPPVVHVADP
                     QETTLPNGLRVIVVENHKLPVVSVQMRFDHPPVAQGEMVGYQDLMGELLTAGTVRRTK
                     EQLDEEVDRLGAQLSGSSDGLFASSLKRNFPALMGIVYEVVTAPLFPPAEFEKARTRY
                     LSGLKMRPEDPDQIADAAGRALTYTKRHPYGEVPTEATVAKITRQQLVAYYERFFRPQ
                     DGYLVFVGDITSAEATALATRLFGTWTGAKVKSEVGPDGVETVQGLGPVSFTAARPEA
                     RLPRRVAFVDRPGSAQSVIRVVFPVALEPKDPEALAAQVLNTILGGGVFNARLMQNLR
                     EKHAFTYGAYSQLDADRWCGAFSAGCSVRNAVTDSAVTEIMNEIMRIRQEPVTAEELA
                     LAKSYMAGSFARSLEDPRTIARFALNTRLFGLKPDHYSTYLQRLDTVSAASVLAAAQR
                     LLKPDNATILVVGDKQETANKLTGLGAEKVILYFDANGDPYRETAALPEKGRTAQ"
BASE COUNT          685 a         1145 c         1077 g          608 t
ORIGIN      
        1 aaacggccag gtcgaagaaa ctcgccggta caggggcccc ctgatcgcgc accgagggct
       61 ccgttgccag cagcgggcgg ggcgcgaaga ggatgcgcgt ggtggatgcc ggattcaccc
      121 gtacgcgttc gccatgccca tccacgtaat gcaattgccc atccaccagc cgttccaggt
      181 tgcggtgtgc tttgaggatg ctggccgggt cgccaagcgt gagcccccag aggtcggcga
      241 tgtaactctt ggccccgcag ttcattcctt cgatgatctc ctgcttgctg catcctccca
      301 gcagctccac ccgccgttca agcagctcgg ccggcacggg atccaccttc cagacggcct
      361 ggcggatgtg tgccgtctcc tccagccgtt ccacctggcc ctggcggaag agctcgcgac
      421 gctgcccgcg ggcttccaac aaacgtgaac gctcctcacc gaagcggtgc gccatgtcct
      481 gtaccagctt caccagttct ccggtgatca cccggtcttc catgggatgt ccagatcggg
      541 gcgtttcggg catcgggtga ccgtgggcaa tgggtgtgcg gccgaaagta ggcatggtgg
      601 atacattcgc gccttcattc cccgcatgcg cggtcctgca cctgcgcggc accgataccg
      661 atgcaacgcg aactactcac ctccgttctc accatggccg tccttactgc cggggcccaa
      721 gcccgcaaga tcgccttttc cgaattcgat cttccaaacg ggttgcacgt gatcctgcac
      781 gaggatcacg gcacgccgat cgtcgccgtc agcgtgatgt accatgtcgg cagcaaggac
      841 gaggccagca accgccgcgg tttcgcgcat ttcttcgaac acctgctctt cgagggcagc
      901 gacaacatcg cccggggcga gttcagccgc tacgtggaga aggccggtgg cgtgctcaat
      961 gccaatacca atggcgaccg tacctattac tacgaggtac tgcccagcaa ccagttggaa
     1021 ctcggtctct ggctggagag cgaacgcatg atgcacgccc gcgtggacca gatcggtgtg
     1081 gatacccagc gcgaggtggt gaaagaggag cgcaggcagc gctacgagaa ccaaccgtac
     1141 ggcagcctgc tgcccgaggt gctgaagagg gcctataccg aacatcccta ccagtggccc
     1201 accatcggtt tcatggagga cctgaacgca gccagcgagg cggactacaa gacgttctac
     1261 aagaccttct acgtgccgaa caacgccgtg ctggtggtgg cgggggacat cgacccccaa
     1321 ggcacgcgtg cgctggtcga gaagtatttc gccgcgatcc cgaaaggaga taaaataccc
     1381 cgtaatactg tggttgagcc tccgctgggg accgagctgc gcgatgtggt gcatgatcgg
     1441 atccaactcc ccgccgtggt gctggcctat cgcataccgg gctatggcac caaggacttc
     1501 tatgcggtgg acatgctcaa caggctgctc agcaacggca acagcagccg attgaacaag
     1561 cgcgtgaagg accaggaaca gaaggcgttg tacgtgggtg cgttcagctt tccgctcgag
     1621 gatcccgggc tggccatcgc gtttgccatc gccaacggcg gggtgtcgcc cgattcgttg
     1681 gagcatgcca tggacgaggt gttcgccgag gtacgcgaga agggcgtacc cgcggccgaa
     1741 ctcgagaagc tgaaggccgg tctggagacc gaattcgtga atgccaacgc gcgcgtggcc
     1801 ggcatcgccg agaacctcgc cagcgcgtac accttcctgg gctccaccgc caaggtgaac
     1861 actgagcttg accagtacat gcatgtgacc accgacgatc tgcagcgtgc cgcccagcac
     1921 tacttcgacc cgaagaagcg cgtggtgctg cattacctgc ccatggtcgg cgataaatga
     1981 tcccgtaaag agcaaccacc atgactttcc acgccctccg cgccctcgcc gccgtgagcg
     2041 tcctgtccat gtccatctcg atgaccgccc agatcgaccg cacccgcgcc ccgaaacccg
     2101 gcccgccccc cgttgtccat gtggccgacc cccaggagac caccctgccc aacggtcttc
     2161 gtgtgatcgt ggtggagaac cacaagttgc ccgtggtgag cgtgcagatg cgctttgacc
     2221 acccgcccgt ggcccagggg gagatggtcg gataccagga cctgatgggc gagttgctca
     2281 ccgcaggaac ggtacgccgc accaaggagc aattggacga ggaggtggac cggctcggtg
     2341 cccagctcag cggaagcagc gatggtctgt tcgcctccag cctgaagcgc aatttccccg
     2401 cactgatggg catcgtgtac gaggtggtca ctgcgcctct cttcccgccg gccgaattcg
     2461 agaaggcccg tacgcgctac ctgagcggat tgaagatgcg ccccgaggat ccggaccaga
     2521 tcgcggacgc cgcaggacgt gcgctcacgt acaccaagag gcatccctat ggcgaagtgc
     2581 ctaccgaggc cactgtggcg aagatcacgc gccagcaact ggtggcctac tacgagcgct
     2641 ttttccgccc gcaggatgga tacctggttt tcgtgggcga catcacctcg gccgaggcta
     2701 cggcactcgc caccagacta ttcggaacat ggacgggcgc caaggtgaaa agcgaggtcg
     2761 ggcccgatgg tgtggagacc gtgcagggcc tgggccccgt atcgttcacg gccgcccgcc
     2821 cggaggcccg gttaccgcgc cgcgtagcct tcgtggaccg accgggcagt gcgcagagcg
     2881 tgatccgcgt ggtctttccg gtggccttgg aacccaagga tcccgaagct ctcgccgccc
     2941 aggtgctcaa tacgatactc ggtggtggtg tgttcaatgc ccgcctgatg cagaacctgc
     3001 gagagaagca tgcgttcacc tacggggcct attcgcagct ggatgccgac cgctggtgcg
     3061 gcgccttcag cgcgggatgc agcgtgcgca acgccgttac cgacagcgcg gtcacggaga
     3121 tcatgaacga gatcatgcgc atccgtcagg agcccgtgac cgcggaagaa ctcgcactgg
     3181 ccaagagcta catggccggc agtttcgccc gttccctgga ggatccgcgc accatcgccc
     3241 gcttcgcgct caatacacgg ctcttcggac tgaaaccgga ccattattcc acctacttgc
     3301 aacgactgga caccgtgagc gctgcttcgg tactggcagc cgcccaacgc ctgctgaagc
     3361 ccgacaacgc caccatcctc gtggtcggcg acaagcagga gacggccaac aaactcaccg
     3421 ggctgggagc ggagaaggtg atcctctact tcgacgccaa tggcgatcct tatcgcgaga
     3481 ccgctgcgtt gcccgagaaa ggccgtaccg cgcaa
//
LOCUS       DAOQOC010000166         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13042_length_3497_cov_3.276002, whole genome shotgun sequence.
ACCESSION   DAOQOC010000166 DAOQOC010000000
VERSION     DAOQOC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13042_length_3497_cov_3.276002"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1382
                     /gene="pcaF"
                     /locus_tag="PL106_03725"
     CDS             174..1382
                     /gene="pcaF"
                     /locus_tag="PL106_03725"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNA32209.1"
                     /translation="MKDAFLVDAVRSPIGSFGGSLAPVRADDLAAHVLRELIKRHPSL
                     DPSALDDVILGCANQAGEDNRNVARMAALLAGLPVSVPGETVNRLCASGMAAVVGAAR
                     AIAAGHGELFIAGGVEHMTRGPWVISKTSTPFGRDAQMFDSSFGWRFVNPVMKEKFGV
                     DAMGETAENLVDANSSITRADQDRFALWSQRKAAQARDNGRLAREIVPVSIPQRKGDP
                     LLVAQDEFLRPGSTIEGLAALKPAFRKNGSVTAGNASGLNDGAAAVLVASEQAIKDHG
                     LRPFARIVSSAVTGVEPRIMGIGPVNATKLALKRAGLSLEQMDIIELNEAFAAQVLAC
                     TRGLGIADDDARINPNGGAIALGHPLGMSGARLLQTAAIELGERNARYALCTMCIGVG
                     QGYATIIERS"
     gene            complement(1409..>3497)
                     /locus_tag="PL106_03730"
     CDS             complement(1409..>3497)
                     /locus_tag="PL106_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32210.1"
                     /translation="YFTPDTTFGVADTLYNGAATSPTMKVPNVHGRDLFLRFEPAQDV
                     LLASQRTAPMVMYDGMAQLHGLTELRPKGMTGAGLVDFSNATLKSTLFQFETMKLHAD
                     TSDFRLTEGDTSSIALKTDNVNATVKLDERIGEFTSNGDNTKVEFPVNQYICFMDRFK
                     WFMDQGDLELESDAKTASVNEDLQLTGTNFVSTRPEQDSLSFMAPKARYDLKKHLITA
                     NDVPYIQVADALIVPDSNRVRIRRNAEMDPISNAVITANSVTRYHRIYNANVNIRKKR
                     EYTAYGDYDYVDETEKAFKIHFTDIRPDTSYQTRAKGRISEDEKFQLSPAFDYFGDVA
                     LAAAEKDLTFTGSTRILHDCPGIARNWMRFSGPIDPKEVFIPVGDSLRDDTGLDIGAG
                     VYLTTDDPFHPYPTFLSRVTSKGDRTIISAKGLLHYDKTKKSYLIGSKDKIRQPDLPG
                     DLVSLNTVNCDLSANGHISTGTELGQVKAEPFGTLDLVEGDSARAAAHVSLYLDFFFD
                     DNALEKMTDAIMAYPEQKAVIVPSTPYERSMREVLGKEKSDKLISELSIKGEIKRLPD
                     ELVRSIVLADVKFRWNSDDGSWQSQGPIGVATILKRPVFRTIKGKIEFQRKRSGDAMT
                     VLLMLDDQTWWFFSYTRGQMLTYSSDAAFNTRISELKEDKMKVEGKKDAPDYYFMLTG
                     KRKVDDFRDRFGL"
BASE COUNT          586 a         1076 c         1116 g          719 t
ORIGIN      
        1 aaggagggcg atgtgctgac cgccgtcgcg gaggaaaaaa gcctccgtcg cagcatcggc
       61 gtgtaccaca tcaccgtgac cgaccagcac ggcgaacagg tcgccctgtt caaaggcacc
      121 gttttccgga ccggcaagcc gtggttcccg gacgaaccgc ttccttcggc cccatgaaag
      181 acgctttcct tgttgacgcc gtgcgctccc ccatcggttc gttcggtggc agcctggctc
      241 ctgtgcgggc cgatgacctg gccgcccacg tgctccgcga gctcatcaaa cgccacccat
      301 cactggaccc gtccgccttg gacgatgtga tcctgggttg cgccaaccag gcgggcgagg
      361 acaaccgcaa cgtggcacgc atggccgcct tgctggccgg cctgcctgtg agcgtccccg
      421 gcgaaaccgt caaccggctc tgtgcttcgg gtatggccgc cgtggtgggc gcggcccggg
      481 ccatcgccgc cgggcacggc gaattgttca tcgccggtgg tgtggagcac atgacgcgcg
      541 gcccctgggt gatcagcaaa accagcacgc ccttcggccg cgatgcgcag atgttcgaca
      601 gcagcttcgg atggcggttc gtgaaccccg tgatgaagga gaagttcggg gtggatgcca
      661 tgggtgagac cgccgagaac cttgtggacg ccaacagctc catcacccgc gcggaccagg
      721 accgcttcgc cctgtggagc caacggaaag ccgcgcaagc acgggacaac ggccgactcg
      781 cgcgcgagat cgtacctgtg agcataccgc agcgcaaagg ggatccactc ctcgttgccc
      841 aggatgaatt cctgcgaccg gggtccacga tcgaaggact tgccgcgctg aagcccgcct
      901 tccgcaagaa cggctcggta acggccggca acgccagcgg cctgaacgat ggtgcggcgg
      961 ccgtgctcgt ggcttcggaa caggcgatca aggatcatgg gctcaggccc ttcgcacgca
     1021 tcgtttcaag cgcggtcacg ggtgtggagc cccggatcat gggcatcggc ccggtgaatg
     1081 ccacaaaact cgcactgaag cgggccgggc tctcgttgga acagatggac atcatcgaat
     1141 tgaatgaggc cttcgccgcg caggtactcg cctgcacacg aggtctgggt atcgccgatg
     1201 acgatgcgcg catcaacccc aacggtggtg ccatcgccct ggggcacccc ttgggcatga
     1261 gtggagcgcg cttgttgcag accgcggcca ttgagctggg cgaacggaat gcgcgctacg
     1321 ccctctgcac catgtgcatc ggcgtgggcc agggctacgc caccatcatc gagcgcagct
     1381 gatcgcaccg cgacccaggg ttcccggatc agaggccgaa gcggtccctg aagtcgtcca
     1441 ccttgcgctt gccggtgagc atgaagtagt agtcgggcgc gtccttcttg ccctccacct
     1501 tcatcttgtc ctccttcaac tcgctgatcc gcgtattgaa agccgcatcg ctgctgtacg
     1561 tgagcatctg cccgcgtgtg taactgaaga accaccaggt ctggtcgtcc agcatcagga
     1621 gaacggtcat ggcatcgccg ctgcgcttgc gctggaactc gatcttgcct ttgatggtgc
     1681 ggaacaccgg tcgcttcagg atggtggcca cgccgatcgg cccctggctt tgccagctgc
     1741 cgtcatcgct gttccaacgg aacttcacgt ccgccagtac gatcgagcgc accagttcat
     1801 cgggtagtcg tttgatctcg cccttgatgc tgagttcgct gatgagcttg tcgctcttct
     1861 ccttgcccag gacctcgcgc atactgcgtt cgtacggcgt actggggacg atgaccgcct
     1921 tctgctcggg gtaggccatg atggcgtcgg tcatcttctc cagcgcattg tcgtcgaaga
     1981 agaaatccag gtagaggctc acgtgcgcgg ccgccctggc actatcgcct tccaccaggt
     2041 cgagcgtgcc gaacggctcg gccttcacct gccccagttc cgttcccgta ctgatgtgcc
     2101 cattggccga caggtcgcaa ttcacggtgt tcaagctcac gaggtcgccc ggaagatcgg
     2161 gctgacggat cttgtccttg ctgccgatca ggtaactttt tttcgtcttg tcgtagtgga
     2221 gcagtccctt ggccgaaatg atggtgcggt cccccttgct cgtcacccgg ctcaggaagg
     2281 tcgggtacgg gtggaaggga tcatcggtgg tgaggtacac gcccgcaccg atgtccaggc
     2341 cggtatcgtc gcggagcgaa tcacccacgg gtatgaacac ctccttgggg tcgatgggac
     2401 cggagaagcg catccagttg cgggctatgc cggggcagtc gtgcaagatg cgggtgctgc
     2461 ccgtgaaggt gagatccttt tccgccgcgg ccagtgccac atcgccgaag tagtcgaaag
     2521 ccgggctcag ttggaatttc tcgtcctcgc tgatccgccc cttggcgcgg gtctggtagc
     2581 tggtgtcggg ccggatgtcg gtgaaatgga tcttgaacgc cttctccgtt tcgtccacat
     2641 agtcgtagtc gccgtacgcc gtgtattcgc gcttcttcct gatgttgacg ttggcgttgt
     2701 agatgcggtg gtacctggtg acgctgttgg ccgtgatcac cgcattggag atcgggtcca
     2761 tctcggcgtt cctgcgtatg cgcacgcggt tgctgtccgg aacgatgagc gcgtcggcca
     2821 cctggatgta cgggacatcg ttggccgtga tcaggtgctt cttcaggtcg tagcgcgcct
     2881 tgggcgccat gaagctgagg gagtcctgtt cggggcgggt gctcacgaag ttggtgccgg
     2941 tgagttgcag gtcctcgttc acgctggccg tcttcgcatc gctttccaac tccagatcgc
     3001 cctggtccat gaaccacttg aagcggtcca tgaagcagat gtactggttc accgggaact
     3061 ccaccttggt gttgtcgccg ttgctggtga actcgccgat ccgttcgtcc agcttcacgg
     3121 tggcgttcac gttgtccgtc ttgagcgcga tgctggaggt gtccccttcg gtaaggcgga
     3181 agtcgctggt gtccgcgtgc agcttcatgg tctcgaactg gaacagcgta ctcttcaagg
     3241 tggcgttgct gaagtccacc aggccagcgc cggtcatgcc cttgggtcgc aattcggtaa
     3301 ggccgtgcaa ctgggccatc ccatcgtaca tgaccatggg cgcggtgcgc tggctggcga
     3361 gcagcacgtc ctgtgcgggc tcgaatcgca ggaacaggtc ccgaccatgc acgttgggca
     3421 ccttcatggt ggggctggtg gctgcgccgt tgtacagggt atccgctacg ccgaacgtgg
     3481 tatcaggtgt gaaatag
//
LOCUS       DAOQOC010000167         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13061_length_3494_cov_3.373655, whole genome shotgun sequence.
ACCESSION   DAOQOC010000167 DAOQOC010000000
VERSION     DAOQOC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13061_length_3494_cov_3.373655"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PL106_03735"
     CDS             complement(<1..698)
                     /locus_tag="PL106_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32211.1"
                     /translation="MIHQRIPVLLSMIAILASISPARSQTQPSLDWSPVMDVDAAGEG
                     GLRPRIALNAEQRPVVLWGDNDPMTNRVAVGNGLSFGAATEVSGPGWAPAVADWMGSS
                     IASMGDTVWVVMKSTPEEQRPVYVRASYDGGASWGDTLRVDPYDGLVSRFPSIAVNEQ
                     GEPLVQYMQFDSGYNGARQVMCRWNGAGFDPPVQLSTPFPAGLVCDCCPNQALIDGTR
                     AVGLYRNADQNDRV"
     gene            1042..1269
                     /locus_tag="PL106_03740"
     CDS             1042..1269
                     /locus_tag="PL106_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNA32212.1"
                     /translation="MADTWLTSLITATPQEGYELAIKLSRMGVKYTQPSAEVRDKLRA
                     DYANNADSLTAASQVIAINFQTVAAANNYWR"
     gene            1455..1919
                     /locus_tag="PL106_03745"
     CDS             1455..1919
                     /locus_tag="PL106_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNA32213.1"
                     /translation="MITAATIAQHFRDVHFGGNWTCVNVRDTLADVTMREAQHQREGF
                     NTIAALTFHINYFVAAVLKVLEGGPLDAHDKFSFTHPPIGSEADWQRLRDKVLSDAEH
                     FATLVEQLPDDRLHADMADPKYGTWYRNLHGIIEHTHYHLGQIVILKKLVRS"
     gene            complement(1928..>3494)
                     /locus_tag="PL106_03750"
     CDS             complement(1928..>3494)
                     /locus_tag="PL106_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA32214.1"
                     /translation="DLGGFGKDFVTGMLATERTGAGHSFSVAIAAHTGIGTLPILYYG
                     NDAQRKKYIPKLASGEWKACYCLTEPGSGSDANSGKTKAVLSADGKHYVLNGQKMWIT
                     NGGFADVFTVFAKIDDDENLSAFIVEKDFGGITLNPEEHKMGIKGSSTRQVFFNDCKV
                     PVENLLSERQNGFKIAVNILNIGRIKLAGATLGGSKKTIDLSVQYANERQQFGRPISS
                     FGAIRQKLADMAVYAYVTESALYRCSRDMENAIEALEAGGADHAKALLKGVEQYAAEA
                     AILKVAGSECLDFIVDEGVQIYGGMGYSAESPVERAYRDSRINRIFEGTNEINRMLTV
                     DMLLKRAMKGQLDLLGPAQAVAAELMGIPDMPEPDDSLLADEKRMVANFKKAVLMTAG
                     GAAQKLGLELAKHQETLMHIADMVIDTYMAESVLLRTLKLAEMRGVNGANGEGSEKVA
                     EQLAMCQLYIHDAADRIHKYAKEAVNNFAEGDEQRAMLMGAKRFAKNTPINTAELRKL
                     VAKKMIAEGKYCY"
BASE COUNT          615 a         1152 c         1002 g          725 t
ORIGIN      
        1 atgacgcggt cattctggtc cgcgttgcgg tagagcccga cagcccgtgt gccatcgatc
       61 agcgcctggt tcggacagca gtcgcagacc aggccagccg ggaacggggt gctcagttga
      121 accggtggat cgaagcccgc accgttccac cggcacatga cctgtcgcgc tccgttgtac
      181 ccgctatcga attgcatgta ctggaccagt ggctccccct gttcgttcac ggcgatggac
      241 gggaagcggg agaccaggcc gtcgtaaggg tccacccgca gcgtgtcgcc ccagctcgca
      301 ccgccgtcgt aggatgcccg tacgtatacg gggcgctgtt cctccggagt ggatttcatt
      361 accacccaaa cggtatcgcc catcgaagcg atgctgctgc ccatccaatc ggccaccgcc
      421 ggcgcccacc cgggtccgct cacttccgtc gctgcaccga aggaaagccc gttgcccacc
      481 gcgacccggt tggtcatggg gtcgttgtcg ccccatagca ccaccggccg ttgctccgcg
      541 ttcaatgcga tccgcggacg gagtcctcct tcccccgccg catccacatc cattaccggg
      601 ctccaatcaa gcgatggctg tgtctggctt cgcgcagggg agatgcttgc caggatcgcg
      661 atcatggaca gcaggacagg gatcctctgg tgtatcatga caggggcgta tgatctgtgg
      721 gataccgcaa tactaagcta ccgcacgtgg agtgtcctgc ggttcggagg ggagttccaa
      781 ggacaaccgg agttgcgtga tcgcgccaag tcattccacc acgaacaggc cccgcacatg
      841 gccttcccgg tcatggacag ccggagtgta cgtccctggt gccagcttgc ccacctggag
      901 gtcgatcgtt cgttgtccgt tgctggcgca ttcgagaagt ggatggggta cgtcgcctca
      961 cccgactgtc ggatgttgcg taacctgtca cagttcagtt ccggggtgtt gtacgtttga
     1021 gcgcatcaag gacgcatcat catggcagac acctggctca cctcgctgat caccgccacc
     1081 ccgcaagaag ggtatgaatt ggccatcaaa ctctcgcgca tgggggtgaa gtacacgcag
     1141 ccctcggccg aggtgcgcga caaactccgg gcggactacg ccaacaacgc cgacagcctt
     1201 acggcagcat cacaggtgat cgccatcaat ttccagacgg tggccgctgc gaacaactat
     1261 tggcgataga ggaagcgcac gtcttatcgc tgaaaggccc tttgcaggaa gcgcacgcca
     1321 cgcagtattt cctccacccg ctctgcggaa agcgtgccga cccgcccgca agggttccat
     1381 cgggatggcc agggcccgtg ataccgccgc ggcgctctgt ccagacaggt cgtccatggt
     1441 cgtagtttta cggtgtgatt accgccgcca ccattgccca acacttccgc gatgtccact
     1501 tcggcggtaa ctggacctgc gtgaacgtgc gtgataccct cgcggacgtg acgatgaggg
     1561 aggcccagca ccagcgcgag ggtttcaaca ccatcgcggc gctcaccttc cacatcaact
     1621 atttcgtggc cgcggtgctc aaggtcctgg agggcggacc gctcgatgca cacgataagt
     1681 tcagtttcac acaccctccg atcgggagcg aggccgattg gcagcggcta cgcgacaagg
     1741 tattatcgga tgcggagcat ttcgcgacgc ttgtagagca gttgcccgat gatcgcttgc
     1801 atgccgacat ggccgatccc aagtacggca cgtggtaccg caacctgcac gggatcatcg
     1861 agcacacgca ctaccacctg gggcagatcg tgatcttgaa gaagctcgtg cgatcgtgat
     1921 caggagatca gtagcaatac ttcccctccg cgatcatctt cttcgccacc agcttccgca
     1981 actccgccgt gttgatcggc gtgttcttcg cgaagcgctt ggcgcccatc agcatcgcgc
     2041 gttgctcatc gccctcggcg aagttgttca cggcctcctt ggcgtacttg tggatccggt
     2101 ccgccgcatc gtggatgtag agctggcaca tggcaagttg ctccgctact ttttcactac
     2161 cctcaccatt cgcaccattc actccccgca tttctgccaa cttcaacgtc ctcagcagta
     2221 cactctcggc catgtaggtg tcgatcacca tgtcggcgat gtgcatcaag gtctcctggt
     2281 gcttggccag ctccagcccc agcttctgcg cggcaccacc ggcggtcatc agcacggcct
     2341 tcttgaagtt cgccaccatg cgcttctcat cggccaacaa ggaatcgtcc ggctccggca
     2401 tgtcagggat ccccatcagc tcggcggcca cggcctgcgc cggaccaagc agatcgagct
     2461 ggcccttcat cgcacgcttc agcagcatgt ccaccgtgag catgcggttg atctcgttcg
     2521 ttccctcgaa gatccggttg atcctgctat cgcggtaggc gcgctccacg gggctttcgg
     2581 cgctgtagcc catgccgccg tagatctgca cgccttcgtc cacgatgaag tcgaggcatt
     2641 cgctgccggc caccttcaat atcgcggctt ccgcagcgta ttgctccacg cccttcagta
     2701 gtgctttcgc atggtcggca ccaccggcct ccagcgcctc gatcgcattc tccatgtcgc
     2761 ggctgcagcg gtagagcgcg ctttcggtca cgtaggcgta tacggccatg tcggccagct
     2821 tctgccggat ggccccgaag ctgctgatcg gcctgccgaa ctgctggcgc tcgttggcgt
     2881 attgcaccga gaggtcgatc gtcttcttcg agccgcccaa cgtggcaccg gccagcttga
     2941 tccgcccgat gttcaggatg ttcaccgcga tcttgaagcc gttctgccgc tcgctcaaca
     3001 agttctccac tggcaccttg cagtcgttga agaagacctg ccgcgtgctg ctgcccttga
     3061 tccccatctt gtgctcctcg gggttcagcg tgatcccgcc gaagtccttc tccacgatga
     3121 aggcgctcaa gttctcatca tcgtcgatct tggcgaaaac ggtgaacaca tcggcgaagc
     3181 caccgttggt gatccacatc ttctggccgt tcagcacgta gtgcttgcca tcggcgctca
     3241 gcacggcctt ggtcttgccg ctgttcgcat cgctgccgct gccgggttcg gtgaggcagt
     3301 agcaggcctt ccattcgccg ctggccagct tggggatgta cttcttgcgt tgcgcatcgt
     3361 tgccgtagta caggatcggc agggtgccga tgcccgtgtg cgccgcgatc gccacactga
     3421 agctgtggcc cgcaccggtg cgctccgtgg ccagcatgcc cgttacgaag tccttgccga
     3481 aaccgcccag gtcc
//
LOCUS       DAOQOC010000168         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13075_length_3492_cov_3.482106, whole genome shotgun sequence.
ACCESSION   DAOQOC010000168 DAOQOC010000000
VERSION     DAOQOC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13075_length_3492_cov_3.482106"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PL106_03755"
     CDS             <1..1026
                     /locus_tag="PL106_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNA32215.1"
                     /translation="LTPRFPLGTSKGTITSRTLWYLIAWDPARPGIKGIGEAALFPGH
                     SKEFPADVRVKLMELCTNTSDWTDRLTGDLVDRPSVRFAVEQCLKDLDAGGSKVLFPS
                     DFTLGRKGVPINGLVWMGDKARMRADIRSRIDTGFSTVKMKIGALAIEEELALLADVR
                     REFGAGNITLRVDANGAFDAHTAPEVLKRLADLRVESIEQPVPPGLYELMAELCVASP
                     LPIALDEDLIGLNTVQAKRDLLDNVRPQRIVIKPGLVGGWTAAEEWIALADARGIGWW
                     VTSALESNIGLNAIAQWAAMIGVKGTQGLGTGSVFLDNIPAPLEVERGALHYRPERPW
                     DLSRIID"
     gene            1042..2145
                     /locus_tag="PL106_03760"
     CDS             1042..2145
                     /locus_tag="PL106_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA32216.1"
                     /translation="MMSTGPDTFNRLTVDGITRRGPEMLRWADELVERHGSASWALDL
                     RATLARLMADGSIDVRTSGTTGAPRAFRSGIQDILASVALTRDAFGLVEGDRALLCVP
                     SGFVAGKMMLARAMVLGLDLHVIDPQGGVLNNLRTTDRFRFAAMIPLQLHTALMHDRA
                     RIDEQFDTILLGGGPVSDGLVAMLRGMRTRIVLGYASTETLTHVALRPLNGPDASDVY
                     TAQRDVTFASGGQGCLVVRTPHLSVKEHVTNDAVEVLDERHMRWLGRLDHVILSGGRK
                     IHPERLEATTEGIIPIPHFFAALPDERLGQAVVLVLETGAPGSAPSMELMERINAALH
                     EHDRPRRVITLRSFIRTGTGKVDRNSTLALAVG"
     gene            complement(2161..2307)
                     /locus_tag="PL106_03765"
     CDS             complement(2161..2307)
                     /locus_tag="PL106_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HNA32217.1"
                     /translation="MAVEGYCVKCKAKREMKDAKENTMANGRKAMKGTCTKCGTGMFK
                     ILGK"
     gene            complement(2445..2900)
                     /locus_tag="PL106_03770"
     CDS             complement(2445..2900)
                     /locus_tag="PL106_03770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA32218.1"
                     /translation="MHTITWHTKHFSELTGSEVHDILRLRVDVFVVEQRCVYAEVDGQ
                     DTEAVHVMGQAGDGGLVAYARILPPHGDGLPHIGRVVVHPEHRGQQLGRALMHRTMAA
                     LEMLYGSRRSALAAQEYLVGFYNSFGFRAVGGSYLWDGIAHVDMEHDLA"
BASE COUNT          619 a         1063 c         1145 g          665 t
ORIGIN      
        1 cttacacccc ggttcccgtt gggcacctcg aaaggcacca tcacctcgcg cacgctgtgg
       61 tacctgatcg cttgggatcc ggctcggccg gggatcaagg gcattggcga ggccgcgctg
      121 ttccccggtc atagcaagga gttccccgcc gatgtgcgtg tgaagctgat ggagctgtgc
      181 acgaacacgt ccgactggac ggaccggctg accggcgatc tggtggatcg gcccagtgtg
      241 cggttcgccg tggagcaatg cctgaaggac ctcgacgcgg gtggcagcaa ggtgctcttc
      301 ccatcggact tcacgctggg gcggaagggc gtgcccatca acgggctcgt atggatgggc
      361 gacaaggcga ggatgcgggc ggacatccga tcgcgcatcg acaccggttt ttccaccgtg
      421 aagatgaaga tcggcgccct tgccattgaa gaggagctcg cgttgttggc ggatgtgcgg
      481 cgtgagttcg gcgccgggaa catcaccctg cgggtggacg cgaacggtgc attcgacgcg
      541 catactgcac ctgaggtatt gaagcgcttg gcggacctgc gggtggagag tatcgagcag
      601 ccggtaccgc cgggcttgta tgagctgatg gccgaactgt gcgtcgcatc gccactgccg
      661 atcgcgctgg acgaggacct gatcggcctg aacacggtac aggccaagcg cgacctgctg
      721 gacaacgtgc ggccgcagcg catcgtgatc aagcccggcc tggtgggcgg atggacagcg
      781 gccgaggagt ggatcgcact ggccgatgcg cgcggcatcg gctggtgggt caccagtgcg
      841 ctggagagca acatcgggct gaacgccatc gcccaatggg cggccatgat cggcgtgaaa
      901 ggcacacagg gcctgggcac cgggagtgtg ttcctggaca acatccccgc gccgctcgag
      961 gtggagcgtg gtgccctgca ctaccgtccc gagcggccat gggacctgtc ccgcatcatc
     1021 gactgacccg ggttgcccac cgtgatgtcg accggaccgg acactttcaa caggctcacc
     1081 gtggatggga tcacccggcg cggcccggag atgctgcgct gggcggatga gctcgtggaa
     1141 cgccacggct cggcatcctg ggctctggac ctgcgggcca cattggcgag gctcatggcc
     1201 gatggttcga tcgacgtgcg cacgagcggg accacaggcg caccgcgcgc tttccgatcc
     1261 ggcatccagg atatcctggc gagcgttgct ctgacacggg atgcgttcgg cctcgttgaa
     1321 ggggaccgcg cgcttctctg cgtgccgtcc ggattcgtgg ccgggaaaat gatgctggcc
     1381 cgggccatgg tgctgggcct cgacctgcac gtgatcgatc cccaaggggg tgtactgaac
     1441 aacctgcgca caacggatcg tttccggttc gcggccatga tcccgctgca gttgcatacc
     1501 gcgttgatgc acgatcgggc ccgcatcgac gaacagttcg ataccatcct gttgggaggc
     1561 ggcccggtga gcgacggcct ggtggccatg ctccggggta tgcgtacacg catcgtgctg
     1621 ggctatgcca gcacggagac gctcacgcat gtggcgttgc gtccgttgaa cggcccggat
     1681 gcctcggacg tgtacaccgc gcaacgcgat gtcaccttcg catcaggcgg gcaaggatgc
     1741 ctggtggtga ggaccccgca tctttcggtg aaggagcatg tgaccaacga cgccgtcgag
     1801 gtgttggacg aacggcacat gcgctggctg ggccggctcg accatgtgat cctgagtggt
     1861 ggccgcaaga tccatcccga acggctggag gccactacgg aaggtattat tccgatcccg
     1921 cacttcttcg ccgcactgcc ggacgaacgt ttgggtcagg cggtagtgct tgtgctggaa
     1981 accggggctc ccggcagcgc accatcgatg gaactgatgg aacggatcaa tgccgcgctg
     2041 cacgagcacg accgcccacg ccgggtgatc acgctgcgca gcttcatcag aacgggcaca
     2101 ggaaaagtgg accgcaacag cacattggcg ttggcggtgg gctgagcagg gctcgtgatc
     2161 ctacttgccc aggatcttga acatgcccgt gccgcacttg gtgcacgtgc ccttcatggc
     2221 cttgcggccg ttcgccatcg tgttctcttt ggcgtccttc atctcacgtt tcgccttgca
     2281 cttcacgcag tatccttcaa cggccatggt tcacttcggg tgttgttccg ccggatccgg
     2341 acgcgttgcc ggagccgatg gatggttggt tcggagccaa tgtaggcgcg tattcgaccg
     2401 gaacggtgca tgtgtgccgg cagttgtcaa caatgactgt gcgttcacgc caggtcgtgc
     2461 tccatgtcca cgtgtgcgat cccgtcccac agataggatc cgcccacggc gcggaaaccg
     2521 aaggagttgt agaagcccac caggtattcc tgtgcggcca gcgcactgcg acggctgccg
     2581 tacaacattt ccaaggccgc catcgtgcgg tgcatcaggg cgcgcccgag ttgctggccg
     2641 cggtgttccg ggtgaacgac cacgcgtccg atgtgtggaa gcccatcccc gtgcggcgga
     2701 agaatgcgcg cgtaggcgac cagtccacca tcacccgctt gtcccatcac atggacggcc
     2761 tccgtgtcct gcccgtccac ctcggcgtac acgcagcgtt gctccaccac gaacacgtcc
     2821 acgcgcaggc gcaggatgtc gtggacctca gaaccagtaa gctcggagaa atgtttcgtg
     2881 tgccaggtga tcgtatgcat acgggatatc ccggtcgggt ctgcggttcg ttcggtggcc
     2941 ccgagcggtc atcagctccg cgctgaccgg accggttccg gtggaatgtc gcagtgggca
     3001 caaggtatgg ggatgtgcag gtgccttggt gagttacgcg cccggcttga agaacccctt
     3061 cacccttgcc acgttgagtg accttgtcct gacgaaggaa gggccgaagc gcccatacac
     3121 tgccacgctg agcctgccga agcagtagcc aatgccatgc acgctcctct gcagtgtcac
     3181 gctgagcttg ccgaagcgcc catgttctgt cacgctgagc acctcgaagt accaccaggc
     3241 aattgccgca ctgatttcaa cggatcgggt ctgagccgtt ctgtggtgta cagcgcttcg
     3301 gcgcgctcag cgtgacagct cataaaaatc cccaaagctg tgtcacgctg agcctgccga
     3361 agcgctacca tactcctcac gactgtcacg ctgagcgcat tgacccgtcc tattctgggt
     3421 ttacaggttt agggttgtgt ccgaagatag gtttacagtt gcgatgatga cctggcctag
     3481 gtttacagca gg
//
LOCUS       DAOQOC010000169         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13117_length_3486_cov_3.816089, whole genome shotgun sequence.
ACCESSION   DAOQOC010000169 DAOQOC010000000
VERSION     DAOQOC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13117_length_3486_cov_3.816089"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..899
                     /locus_tag="PL106_03775"
     CDS             93..899
                     /locus_tag="PL106_03775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA32219.1"
                     /translation="MQAAVVLRQDASGGLSFLSDLKTDVNRFLFHRELEEGGKRAFFI
                     GLLRTVIAEGRTLTLNSQLGDHPDTALLLAIVKEQGMWATVVDNSACPVLEAASPEEL
                     FAQVDAQRELRYRVNKLRNQKQAEFEVFTDQVDLETWTADFQQAHISRWEGTDTPSRF
                     RDLERRQFLLGCLQAWAANGLLVRFSVRTKDGRTGFVIGLRAPGTIIHHSTTYHPCHA
                     KFSPGKALLHTMAGWMRDNGLRILDFGDGREPYKYSVATSERPLQRIFIG"
     gene            1064..2086
                     /locus_tag="PL106_03780"
     CDS             1064..2086
                     /locus_tag="PL106_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32220.1"
                     /translation="MAADHANWLRACAERTDRVACEVIDLSRDGWKEAVLNGGFDGLL
                     TQPSGWTAAGRDRYLECIRELERTTGLPMNPAPAEIAIYENKRALARWLEEDHWPHPR
                     TDVFTHVEEALSHLRSASFPLVAKVSIGAGGRGVRILRNEEDAMRYVQGTFHGAGASR
                     DALPQWRGGGLLLRVLRMLAQPAALIERVRNYRMLHAEAQRDHVLFQAFVPHTFEWRV
                     VRIGDSFFAHKKLVKDGKASGSLLKEYGDPPRALLDLVRNITEKHGFRSVAIDLFEDP
                     VGGYLVNEVQCIFGQSDPHQMIIDGVPGRYMHHDGAWVFEPGDFNRFECYLLRLDDMI
                     DRITKK"
     gene            2083..3045
                     /locus_tag="PL106_03785"
     CDS             2083..3045
                     /locus_tag="PL106_03785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA32221.1"
                     /translation="MKVLFVHSGNSSRYPVSPFVLSQAEALRKSGVEVSLFPVMGRGR
                     DYLRNVRPLRERIRTERPDVIHAHYALCGWVAVLARSGRPVVVSLMGDDAQGTFDPRG
                     ERTMGGNLLVMGTRLLQPFVQAIIIKSEGMGRAVWRKRIMHLLPNGVDLERFNPTLFG
                     GHTLSATSRAKKCVLFLGDPADPNKNVALVRDAIARSNRTDVELITPYGAGHADVPRL
                     MQEAHVLALCSFAEGSPNVLKEAMAMGLPVVTVPVGDAALVLGDTPGCFVAGYDAGDF
                     ADKLCRALDLKGRTTGRNRLIALGLDAPAVARKLQAIYSSLLTR"
     gene            3079..>3486
                     /locus_tag="PL106_03790"
     CDS             3079..>3486
                     /locus_tag="PL106_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNA32222.1"
                     /translation="MCGIAAIIHTGGAPVDESALRAMMRAMKHRGPDDEGVLLSGNAG
                     LGFVRLGILDLSSNGHQPMTSADGRFTIIYNGEIFNYIELREELRAKGHVFRTGTDTE
                     VLLAAYAEWGRDMLHRLNGMWAFILLDVKERALF"
BASE COUNT          631 a         1045 c         1094 g          716 t
ORIGIN      
        1 gttgcccctt ccaatccccg gctatcctgg gttggttcaa tcggtccatc cgcgatagag
       61 cacggctttt cgcattgcgg gacgatcgag ggatgcaagc ggccgtggtg ctacgccagg
      121 acgcctctgg tggcctgtcc ttcctgagcg atctgaagac ggacgtgaac cgcttcctct
      181 tccaccggga actggaggag ggcggcaaac gagctttctt catcggtctg ctccgaacgg
      241 tgatcgccga gggacgtacg cttaccctga acagccagtt gggcgaccat ccggacaccg
      301 cgctgcttct agccattgtg aaggaacagg gcatgtgggc caccgtggtg gacaattctg
      361 cgtgtccggt gctcgaggcc gcatcacccg aggaactttt cgcacaggtg gacgcccagc
      421 gcgaactgcg ctaccgtgtg aacaaattgc gcaaccagaa acaggccgag ttcgaggtct
      481 tcactgacca ggtcgatctt gaaacatgga ccgcggactt tcagcaggcc cacatcagcc
      541 gctgggaagg tacggacaca ccctcccgct ttcgcgatct cgaacggcgc caattcctgc
      601 tggggtgcct gcaggcctgg gcggcgaacg ggctcctggt gcggttctcg gtgcgtacca
      661 aggacggtcg tacgggcttc gtcatcggat tgcgtgctcc cgggacgatc atccaccaca
      721 gcaccaccta ccacccgtgc cacgccaagt tcagtccggg aaaggctttg ctgcatacca
      781 tggccgggtg gatgcgcgac aacggactcc gcatcctgga cttcggcgat ggtcgcgaac
      841 cgtacaagta ctccgtggcc acgtccgaaa gaccgttgca gcggatcttc atcgggtgac
      901 ggtccgacct gcgcttccgc gcgtcctcgt ccatgatccg catggtgagg ggcaaccagc
      961 tcctgtacgg tatgtaccag cggcatctga aacggcggtg gggatcgtga tggcaggatc
     1021 aagaaccgga catcgtcctc ggctcgccat ccttcgcaac gaggtggccg ccgaccacgc
     1081 caactggttg cgcgcatgcg ccgagcgcac tgatcgtgtc gcctgtgaag tgatcgacct
     1141 gtcgagggac ggttggaaag aagccgtgct caatggtggt ttcgatggcc tgctcaccca
     1201 gccttcgggc tggaccgcgg ctggccgaga tcggtacctc gagtgcatcc gcgaactcga
     1261 gcgcaccacc ggcctgccca tgaaccccgc acccgccgag atcgccattt acgagaacaa
     1321 acgcgcactc gcccgttggt tggaggagga tcactggccg catccacgaa cggacgtctt
     1381 cacacatgtg gaagaggctt tgtcgcacct gcgttcggcc tcttttccgt tggtggccaa
     1441 ggtcagtatc ggtgccggtg gtcgtggtgt gcggatactc cggaatgagg aggatgcaat
     1501 gcgttatgta cagggcacct tccatggagc gggtgcatcg cgtgatgcac tgccccaatg
     1561 gcgcggtggt gggctgctgc tgcgcgtcct gagaatgctc gcacagccag ctgccctcat
     1621 cgaacgcgtc aggaactatc ggatgctgca tgccgaagct cagcgggacc atgtcctctt
     1681 ccaggcgttc gtgccgcata ctttcgaatg gcgcgtcgtg cgcatcggcg attccttttt
     1741 cgcccacaag aaactggtga aggacggcaa ggccagcggc agtctgctca aggagtacgg
     1801 tgatccacca agggccttgc ttgacctggt gcggaacatc acggaaaagc acggcttccg
     1861 ttccgtggcc attgacctgt tcgaggatcc ggtcggcggt tacctcgtga atgaggtcca
     1921 gtgcatcttc ggtcagagcg atccgcatca gatgatcatc gatggtgtgc ctggacggta
     1981 catgcaccac gatggtgctt gggtttttga acccggcgac ttcaatcggt tcgagtgtta
     2041 cctgctgcgc ctggacgata tgatcgatcg gatcaccaag aaatgaaggt gctcttcgtc
     2101 catagcggga attcatcgcg ttatccggta tctccattcg tgctttccca ggccgaagcc
     2161 ttgcggaagt cgggtgtgga ggtctccctt ttccccgtga tgggccgtgg ccgggattat
     2221 ctgcgcaatg tgcgcccatt aagggaacgc atcaggacgg agcgaccgga tgtcatccat
     2281 gcccactatg cgttgtgcgg ttgggtggct gtgctcgcac gaagcgggcg acccgtggtg
     2341 gtctcactca tgggtgacga tgcccagggc acctttgatc cacgtggcga acgcaccatg
     2401 ggtggcaacc tgctcgtcat gggcacgcgc cttttgcagc cgttcgtgca ggccatcatc
     2461 atcaaatccg aaggcatggg ccgtgccgtc tggcggaagc gcatcatgca cctcttgccg
     2521 aatggtgtcg atctggaacg gttcaacccg accttgttcg gtgggcacac gttgtccgcg
     2581 acgtcgaggg cgaagaagtg cgtgctcttt ctcggtgatc ccgccgatcc caacaagaac
     2641 gtggccctgg tccgtgatgc gatcgcacgg tcgaacagga ccgatgtcga gctgatcacg
     2701 ccgtacggtg cgggacatgc cgatgtgccg cgtctgatgc aggaagccca tgtgctggcc
     2761 ctgtgttcgt tcgccgaagg ctcgcccaac gtgctgaagg aggccatggc catgggactg
     2821 cccgttgtta ccgtaccggt cggcgatgcg gcattggtgc tgggcgatac accgggttgt
     2881 ttcgttgccg gatatgatgc cggggacttc gccgacaagc tttgccgtgc gctggatctc
     2941 aagggccgta ccaccggtcg gaaccggttg atcgcgctcg gcctggacgc acctgcggtg
     3001 gccaggaagc ttcaagcgat ctattcttca ctgctcaccc gttgatggcc gaccttcgcg
     3061 gcccttcgat ccaggatcat gtgtggcatt gcggcgatca tccataccgg cggcgctccc
     3121 gttgacgaga gcgcattgcg ggcgatgatg cgtgccatga agcatcgtgg gcctgatgat
     3181 gagggcgtcc tcctcagcgg caatgcgggc ctgggcttcg tgcggctcgg tatcctggac
     3241 ctgtccagca acggccacca acccatgacc tcggccgatg gaaggttcac gatcatctac
     3301 aacggggaga tcttcaacta catcgagctt cgcgaggagt tgcgggccaa gggccatgtg
     3361 ttccgcacgg gcaccgatac cgaagtgctc ctggccgcct atgccgaatg ggggcgcgac
     3421 atgctccacc ggctcaacgg catgtgggcc ttcatcctcc tggatgtgaa ggaacgcgcc
     3481 ctgttc
//
LOCUS       DAOQOC010000170         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13120_length_3485_cov_3.670845, whole genome shotgun sequence.
ACCESSION   DAOQOC010000170 DAOQOC010000000
VERSION     DAOQOC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13120_length_3485_cov_3.670845"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PL106_03795"
     CDS             <1..256
                     /locus_tag="PL106_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32223.1"
                     /translation="HLREVRRVLRPGGTFTAVLRRKTSMENLPFAAHGFTMYEQADWE
                     VVLQRNGFAPVSTTVIQEPGVEYEGKKYRPESLVMVAGFA"
     gene            complement(361..939)
                     /locus_tag="PL106_03800"
     CDS             complement(361..939)
                     /locus_tag="PL106_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 periplasmic binding fold superfamily
                     protein"
                     /protein_id="HNA32224.1"
                     /translation="MKTKLKYLAYALIASTTITACKRDEVDPITPPPPTNEEELITTL
                     RLHFHSVGGAEHKHMEFTDLDGSGGNAPVIHGDTLSADSVYHVHVEVLNESGSPAEDI
                     TQEIEAESDVHQFFYQVSGASATVAYDDADANGHPIGLETVWTVGAASNGTVVVTLRH
                     QPDKTASGVSSGDITYAGGETDIEVSFPLVIE"
     gene            complement(987..3296)
                     /locus_tag="PL106_03805"
     CDS             complement(987..3296)
                     /locus_tag="PL106_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA32225.1"
                     /translation="MLYSLAGVGQMQAQESSCTLVLSGHVIDDHDKEALPFAEVYLAE
                     LDRGAQADSAGRFTIAGLCAGTYRLRAVHIGCEPVERTVKLVASMEILIRLEHHVHEL
                     REAEIVRSRPDEHVGQAHEQLDKDAVERSSGRTLGEMVAVIPGVTTLNSGPTISKPMV
                     HGLTGNRVLILNQGVRQEDQQWGSEHAPNLDPFSSDRITVVKGAAGVQYGSDAIGGVV
                     ITEPVELPRSGPLSGEVRALGMLNGLGIGGNALLQGGVKGVTGMGWRVQGSGRSMADS
                     RAADYNLSNTGMHETGASASIGFRDHRWNASAYGSWYARDLGILRAAHIGNLTDLQNA
                     IASGTPWYVGEPTREIAAPRQEVQHVLAKAEAGYAVSERGRVALTYGYQDDERQEYDI
                     RRGGRSDRPALDLHLVTHTGEAAFKHWIGKHIHGKVGVSGLYQENINIPGTGIRPLIP
                     NYRKQSAGAFVIEHYTITERVELEVGFRLETTHLEVAKYNDSDVLIRPEHRFLNRAIS
                     AGVNWSVRDSTRVRFNLSSAFRPPHVSELYSEGLHHGAAAIEIGDPTLGSERAYKSVL
                     EIGTALLHGRAHASLTAHASLIDNFIYLRPSSLELTVRGAFPVFNYVATDALISGLDA
                     SFDLRLVGPFTWRLRASTVHGRDLVKDEWLFQMPSDRLENTLVYAPRTTGKWRHLEFA
                     TTSHLVLVQERIPVGVDLASPPPTYHLLGLSASASRPLGKGELRLGLRGSNLLNAAYR
                     DYLDRFRYYADARGLEIALWITYRFGSPN"
BASE COUNT          615 a         1112 c         1056 g          702 t
ORIGIN      
        1 ccacctgcgc gaagtgcgcc gcgtgctgag gcccggggga accttcaccg ccgtgctgcg
       61 ccgaaagaca tccatggaaa atctgccctt cgcggcgcat gggttcacca tgtacgagca
      121 ggccgattgg gaagtggtgc tgcaacgcaa cggcttcgct ccggtgagta ccacggtgat
      181 ccaggaacca ggcgtggaat acgaagggaa gaagtaccgg ccggagagct tggtgatggt
      241 ggcggggttc gcgtgaggcc gtttcggccg caacaatggc ctttcgtgcc gaccccatac
      301 tgcccaagcc cgtctgcgtg ccggcgggaa cacgccctca cggcctaccc cgaccaactc
      361 ctactcgatc accagcggaa aactgacctc gatgtccgtt tcgccacccg cgtaggtgat
      421 gtcgccgctg ctgacgccgc tcgcggtctt gtccggttgg tggcgaagcg tgaccaccac
      481 ggtgccgttg ctcgctgcac ctaccgtcca caccgtctcc aacccgatgg gatgcccatt
      541 ggcgtccgcg tcatcgtagg ccaccgttgc gctcgcaccg ctcacctggt agaagaactg
      601 gtgaacatcg ctttccgcct cgatctcctg tgtgatgtcc tcggcggggc tcccgctctc
      661 gttcagcact tcgacgtgta catggtacac actgtccgcg ctgagtgtat cgccgtggat
      721 caccggtgca ttcccaccac ttccatcgag gtcggtgaac tccatgtgct tgtgctcagc
      781 gcctcccacg gaatggaaat ggagcctgag cgtggtgatg agctcctctt cattcgttgg
      841 cggaggcggg gtgatgggat caacctcgtc ccgtttgcac gcggtgatcg ttgtactggc
      901 gatcagcgca taggccaggt acttcagttt ggtcttcatg ctccttggtt cttgttttcg
      961 atgtcgttct tcgttctcct gttcattcag ttggggcttc cgaaccggta ggtgatccag
     1021 agggcgattt ccaggccgcg cgcatcggcg tagtagcgga accggtccag gtagtcgcgg
     1081 taggcggcgt tcaggaggtt gctcccccgt agtccgaggc gcagttcgcc cttccccagc
     1141 ggccgggagg ccgatgccga caggccgagc aggtgatagg tcggtggtgg agaagcaagg
     1201 tccacgccca caggtattct ttcctgtacg aggaccagat ggctcgtggt ggcgaactcc
     1261 aggtgtctcc acttgccggt ggtacgtggt gcgtacacga gggtgttttc caagcggtcc
     1321 gatggcatct ggaagagcca ttcatccttc accagatctc gcccatgcac agtggacgca
     1381 cgcaggcgcc aggtgaacgg tccgacgagg cgcagatcga aggatgcatc aaggccgctg
     1441 atcagtgcgt cggtggccac gtagttgaag acgggaaagg cgccccggac cgtgagttcc
     1501 aagctggatg ggcgcagata gatgaagttg tcgataagcg atgcgtgcgc ggtgagcgag
     1561 gcatgtgccc ggccgtgcag aagtgcggtc cctatctcca gcaccgactt gtacgcccgt
     1621 tcactgccga gtgtggggtc gccgatctcg atcgctgccg caccgtgatg cagcccttcg
     1681 ctgtacaatt cgctcacatg gggcgggcgg aacgcgctgc tcagattgaa gcgcacccgc
     1741 gtgctgtcgc gcacactcca gttcacaccc gcgctgatgg cacggttcag gaaccggtgc
     1801 tccggacgga tcaggacatc ggaatcgttg tacttggcta cctccaggtg cgtggtctcc
     1861 agccggaaac ccacctccaa ctccacacgc tccgtgatgg tgtagtgctc gatcacaaag
     1921 gccccggcgc tctgtttccg gtagttcggt atgagcgggc ggatccctgt ccccgggatg
     1981 ttgatgttct cctggtacag gccggaaacg ccgacctttc catgtatgtg cttgccgatc
     2041 caatgcttga aggcggcctc accggtgtgc gtgaccaggt gtaggtccaa ggccggtcta
     2101 tcgctgcgcc caccgcggcg gatatcgtac tcctgccgtt catcatcctg gtagccatag
     2161 gtgagcgcca cacggccgcg ctcggaaaca gcgtagcccg cctcagcttt ggcgagcaca
     2221 tgctgcactt cctgcctggg cgccgcgatc tcgcgagtgg gctcacccac gtaccatggc
     2281 gttccgctcg cgatcgcgtt ctgcaggtcg gtgaggttgc cgatgtgcgc ggcgcgtaga
     2341 atgcccaggt cccgcgcata ccagctcccg taggccgagg cgttccagcg gtggtcgcgg
     2401 aagccgatgg acgcagaagc accggtctcg tgcatgcccg tattgctcaa gttgtagtcc
     2461 gcagcgcgac tgtcggccat acttcgccca ctaccctgca cacgccaacc catgccggtc
     2521 acgcccttca ccccgccttg caacagggca ttgccaccga tgccaagccc gttcagcatc
     2581 cccagtgcac gtacctcacc gctcaacgga ccactgcggg gtagttccac cggttcagtg
     2641 atcaccacac caccgatcgc atcgctgccg tactgcacgc cggccgcgcc tttcaccacg
     2701 gtgatccgat cactgctgaa gggatccagg tttggcgcgt gttcactgcc ccactgctgg
     2761 tcctcctgac gcaccccttg gttgaggatc aatacccggt tgcctgtgag gccgtgtacc
     2821 atgggcttgc tgatcgtggg gccgctgttc agggtggtca cgcccggtat caccgcgacc
     2881 atttcaccca gcgttcgccc actactgcgt tccaccgcgt ccttgtccag ttgttcatgg
     2941 gcctgtccca catgttcatc gggtctgctg cgtacgatct ccgcttcgcg cagttcatgt
     3001 acatggtgct ccagacggat caggatctcc atgctcgcca ccagcttcac tgtacgttcc
     3061 accggttcgc agccgatgtg gacggcgcgc aaccggtagg tgcctgcaca gagtccggcg
     3121 atggtgaaac ggccagcaga atcagcctgt gcgccacggt ccagttcggc caggtacacc
     3181 tcggcgaatg gcaaggcttc cttgtcgtga tcgtcgatca catggccact gagcacgagc
     3241 gtacaggagc tctcctgggc ctgcatctgg ccaacgccgg caagggaata caacataccg
     3301 aaaaggagtg aatgccgcaa aactcacgtg ataagcaccc tatggcgcat tgaacagacc
     3361 tgaaagcgaa atgatccccg gcagcaccga gggttcatgc tccggcaagg agccgatcag
     3421 caggtcgaag gcgggccgcg gtccgcagcc atggccacgg gcccgaacga gtgcttccgc
     3481 accac
//
LOCUS       DAOQOC010000171         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13132_length_3483_cov_3.468203, whole genome shotgun sequence.
ACCESSION   DAOQOC010000171 DAOQOC010000000
VERSION     DAOQOC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13132_length_3483_cov_3.468203"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PL106_03810"
     CDS             complement(<1..1261)
                     /locus_tag="PL106_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNA32226.1"
                     /translation="MRGNRSFPVKFKILAVLGAAVLVSVAWSTAVMPVRGPHSALELR
                     VLRDQIEGLDVQFGSYFVTSGRCAGCHGHDSLGYAMVAGEGEDVNVANDWRSTMMANS
                     ARDPFFLAKMEHEGLVNPALQAQIENTCLKCHAPLAVFEQQMKGGPAFTAASLDTSVF
                     ARDGVSCLACHMQDPDSAGNFFSGDLHFDSARVWGPYNQEQIHEEIMQFFVGFTPDQG
                     SHILDGRVCAGCHTAINHPVDLEGNPTGTSFYEQTLWQEYVNSIYYGTEQNCRSCHMP
                     RIQDSVVLASGYAFLTGQSPFGKHHLTGGSEFMLKMMKDHIGDFGIPATPTQFDSTIA
                     RSRQLRQTTMSMSLDLIGYTDDTLFVDVTLNNLIGHKFPGGFPNRRAFIRLVALSAAG
                     DTLFQSGGWDGNYEVIGNDLPYEPHHDV"
     gene            1432..2097
                     /locus_tag="PL106_03815"
     CDS             1432..2097
                     /locus_tag="PL106_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32227.1"
                     /translation="MISRPGTDPILRFAVLACILVTTALMVEATALYPGGSMLDVRSV
                     GFDWSKNFFSNLFQERAINGATNPGRPWALVGMAFHSLGDGLFFIRMSRVILHKHSTR
                     VLKVVGISNIAFNFMIATPFHDAMVTTSSTLSLLGLFYITVFIIRTKLHVLKVCCVAF
                     MLVAYFTLFLYGTGNWGLLAIMQKAATISSALLVLGLTFFTAAEDFEGGVPKMDTGAG
                     ASA"
     gene            complement(2133..3020)
                     /locus_tag="PL106_03820"
     CDS             complement(2133..3020)
                     /locus_tag="PL106_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32228.1"
                     /translation="MRHSTLFLSICMAGALGAQPILQYGNVNLVGSNYDLYVVTDPGN
                     SNPGVNGANVTWDFSSAVMVLAGTNSFVAPAGTPYAASFPASNLAAVVTHQDTTGYMY
                     YSVSSSQLEILAEYVGTNDENVYTVPKSVLEFPFAFNDSFQDDYVDEDASNTVTRTYT
                     GYGTVILPGGGTVADVVKTTSTSGSMAFFRSNPVAPLVSIEDDGDAVWWMPAGVGLPE
                     LAASPLHAWPNPVSDALQLRSEPGDQWSVLDVQGRALLSGRATTTLNTIDMAGLSTGH
                     YVLRVANAAGVRAVPVIKQ"
BASE COUNT          685 a         1075 c         1048 g          675 t
ORIGIN      
        1 tcacatcgtg gtgcggctcg tagggaagat cgttgccgat cacttcgtag ttgccgtccc
       61 atccgccgct ctggaaaagt gtatcgcccg cggcggacag cgccaccagc cggatgaatg
      121 cccgccggtt ggggaagccc ccggggaact tgtggccgat gaggttgttg agggtcacat
      181 cgacgaacag ggtgtcatcc gtgtaaccga tcaggtcgag ggacatggac atggtggtct
      241 gccgcaactg gcggctgcgg gcaatggtgc tgtcgaactg cgtcggggtc gccggtatgc
      301 cgaagtcgcc gatatggtct ttcatcatct tcagcatgaa ctcactccca ccggtcaggt
      361 ggtgcttccc gaacggggat tggccggtca ggaaggcgta tccggaagcc agcacaacgg
      421 aatcctggat gcgtggcatg tggcagctcc ggcaattctg ttccgtgccg tagtaaatgg
      481 agttgacgta ctcctgccac aaggtctgct cgtagaagga cgtgccggtg gggttgccct
      541 cgaggtccac cgggtggttg atcgccgtat ggcaacccgc gcatacgcgg ccatcgagga
      601 tgtggcttcc ctggtccggt gtgaacccga cgaagaactg catgatctcc tcatggatct
      661 gctcctggtt gtacggtccc catacacggg cgctatcgaa atgcaggtcg cctgagaaga
      721 aatttcccgc ggaatcggga tcctgcatgt ggcaggccag gcaggagaca ccgtcgcggg
      781 cgaatacgct cgtgtccaaa ctggcggcgg tgaacgcggg gccgcccttc atctgctgct
      841 cgaacacggc cagcggcgcg tggcacttca ggcaggtgtt ctcgatctgc gcctgcaatg
      901 cgggattcac caggccttcg tgctccatct tcgcaaggaa gaacggatcg cgcgcgctgt
      961 tggccatcat ggtactgcgc caatcgttgg ccacgttgac atcctctcct tctcccgcca
     1021 ccatggcgta gcccaacgaa tcgtgcccat ggcaacccgc acagcgcccc gaagtgacga
     1081 agtaggaacc gaactgcaca tcgagaccct cgatctgatc gcgcagcacc cgcaattcca
     1141 acgcactgtg cgggccccgt accggcatga cggccgtgga ccatgccacc gacaccaata
     1201 cggccgcgcc cagtacggcg aggatcttga atttgaccgg gaaagacctg ttgccgcgca
     1261 tcgggtggcc aatttatacg gacggcacgg gcgaaggttg accggcgtca ctccaccaca
     1321 ccgcccggct aacattgcga ccggggtgtt cggtgcgacc actgttaccg gcatcctgtg
     1381 cttcacgcac gacccggacg caaccgatca ttgaacgctg tggaacaacg catgatatcg
     1441 cgacccggaa ccgatccgat cctgcgcttc gccgtgctgg cctgcattct cgttacgacg
     1501 gccctgatgg tggaggcgac cgcgctctac ccgggtggtt caatgctcga tgtgcggtcc
     1561 gtcgggttcg actggtcgaa gaacttcttc agcaacctgt tccaggagcg ggccatcaac
     1621 ggggccacga accccggcag gccatgggca ctggtcggca tggccttcca ttccttgggc
     1681 gatggcctgt tcttcatccg catgtcccgc gtgatcctcc acaagcattc aacgcgcgtc
     1741 ctgaaggtgg tcggcatcag caatatagcg ttcaacttca tgatcgccac accgttccac
     1801 gatgccatgg tgaccacatc gagcacactc tcactgctcg gcttgttcta catcaccgtg
     1861 ttcatcatcc gcaccaagct ccatgtgctc aaggtctgtt gcgtggcgtt catgctcgtc
     1921 gcatacttca ccctgttcct gtacggaaca ggcaactggg gtctgctggc gatcatgcag
     1981 aaagcagcaa cgatcagttc cgcgctactt gttctcggac tgaccttttt cacagcagcc
     2041 gaggacttcg aaggtggtgt tccgaagatg gatacgggag caggggcctc ggcttgatac
     2101 cgaacgggcc cttcgctcgt cgtggccccg gactactgct tgatcacggg cacagcacgc
     2161 accccggcgg cgttggccac acgcagaacg tagtgtcccg tgctcaaccc ggccatatcg
     2221 atggtgttca gcgtggtggt ggcacgaccg ctcagaagcg cacgaccctg tacgtccaga
     2281 acactccact ggtcacccgg ctcagaccgc aattgcagcg catcgctcac ggggttcggc
     2341 caggcgtgca gcggggaggc ggccaactcg ggcaggccca cgcccgccgg catccaccac
     2401 accgcatcgc catcgtcttc gatggatacc aagggcgcaa cgggattgct ccggaaaaag
     2461 gccatgcttc cactggtgct ggtcgtcttc accacatcgg ccaccgtacc gccccccggc
     2521 aggatcaccg taccataacc cgtgtaggtg cgggtcacgg tattcgacgc gtcctcgtcc
     2581 acataatcat cctggaacga atcattgaat gcgaacggga actcaaggac gctcttgggc
     2641 acggtgtaca cgttctcatc attggtgcca acatactccg ccaggatctc gagctggctg
     2701 ctgcttacac tgtagtacat atatcccgtg gtatcctggt gcgtgaccac cgcggccagg
     2761 ttggaagccg ggaagctggc agcatacggt gttccagctg gggccacaaa gctgttggtg
     2821 ccggccagga ccatcacagc actggagaaa tcccacgtga cattggcccc gttcacaccg
     2881 gggttgctgt taccgggatc ggtcaccacg tacaggtcgt agttggaccc gaccaggttg
     2941 acgttgccgt actgcagaat gggctgggca ccgagggcgc cagccatgca aatggacagg
     3001 aacagggtag agtgtcgcat gcggttcgag tttgatgagg atcccggttt ttcagaggtt
     3061 gacgtgaggg aacccccggg ccaaatggcc tctgggttct tgaacatgca cgatggagcg
     3121 ccaaagacca gaaggaacat cggaaaaacc gaccttgggg cagtccgcac gcatcgatcg
     3181 tacgaatgga tcgtccagac ggtatctgtg attcgtggat gccatgatga cccgtgcgaa
     3241 gctatggatg ttgccggatc cgccacaaca cgaggacggt cgaggtgctc ggcacgcccc
     3301 gtcgggagat ccagtccagt gctgatccaa gggtggccca agcgctacgc cgcccggtga
     3361 tccgggcaat ccacacggag ctcgtgcggt gcgagcggtt tgcgcaacaa cgcgcaatag
     3421 gccctgccgt tccgttcgcc gaagtgggca caggtcgcgc acatgctgtc cacccgcacc
     3481 aac
//
LOCUS       DAOQOC010000172         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13349_length_3450_cov_3.508100, whole genome shotgun sequence.
ACCESSION   DAOQOC010000172 DAOQOC010000000
VERSION     DAOQOC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13349_length_3450_cov_3.508100"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PL106_03825"
     CDS             complement(<1..1763)
                     /locus_tag="PL106_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNA32229.1"
                     /translation="MIRSYTLPFLALCSLPATAQYPGLVFDGQNDQVTVPNAVLNSIG
                     TGPFTVEAWVRGVEADQPSHPRILSNRDAVNNGFMFAFHGLWGGSNHKMVNLQLDGLN
                     YILINNGTYNASVLDGTCHHIAVSKGVDSLRFYVDGAHIGSKVLQGNPSVATTAANMV
                     IGNDGPDPHPFNGHLSQLRLWDHVRSPAEILSNKDQSIAGTTPGLVGYWELNEGSGQD
                     IADKTGTTNGLLGTNEVVEDEDPAWNMDGCPVTSMGRCLVFDGADDQVTLSSTAVNAI
                     GTGDFTVEAEIRAVEADQLQHPRILSNRDQVNNGFLFGFHGVWGGSNYKMINMQLDGL
                     NYILINNGSYNGSLMDGTCHHIAVTKSADSLRFYADGMHIGSKVLQGAPSTTTTATSM
                     LIGNDAPDPQPFNGNIAQLRVWNEARSAAEIVANMGVSIPGNTPGLVGYWEMNDAGGQ
                     FVQERQALVPFHLIENRRIVRCLHDFDPHFFQVFPADGHEHRFFRQGYMFGFEADFLA
                     AQVVQQGGALFGAVGIGVAADGVFAQVGGLFQQRGVGVGLAGLFHQCPGHSVRDFGVG
                     RVVVVGFVQDGERLAVALGLV"
     gene            complement(1864..2541)
                     /locus_tag="PL106_03830"
     CDS             complement(1864..2541)
                     /locus_tag="PL106_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32230.1"
                     /translation="MDEGSPSLSSGRRRLQWAILACLLLTVPLMLIAASLYPGGSIAD
                     ANSAGFIWSKNFFSNLFQEKALNGADNPGRPWALVGMAFHSLGDGLFFIRMSRVITER
                     VIRPMLRVVGHLSIGSNFLIATTLHDAAVTTSSTLSLLGLFCITVLLFQSKQHWLKVL
                     AVACMLIFYYTLFLYGMGDWGLLAVMQKVTAGCSTALVLALAFGTKAEDFKKRDRRTD
                     EQKDAIV"
     gene            2759..3223
                     /locus_tag="PL106_03835"
     CDS             2759..3223
                     /locus_tag="PL106_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNA32231.1"
                     /translation="MDRYELLLRPVAHMHPAQILAGLSTSDVVRRVPGVAHNIVEITA
                     HMTFWQDWFLARCSGEALPLVIHATEGWPSVGQKDWELVHHAFLNGLDRAVHHPGSGK
                     LKPPIELGLLAHYEVEDVMAHLAQHNSHHLGQIVVLRQAFGLWPPPGGSFTW"
BASE COUNT          691 a         1054 c         1040 g          665 t
ORIGIN      
        1 aagacaagtc cgagcgctac cgccaggcgc tcgccatcct gcacaaaacc cactacaacc
       61 acccttccga cgccgaaatc gcgcaccgaa tggcctgggc actgatggaa caggccggcc
      121 aacccaacgc cgacgccacg ctgctgaaac aagccgccga cctgtgcgaa gacgccatcc
      181 gccgccaccc cgatacctac ggcgccaaac agtgcgccgc cctgctgtac gacctgcgcc
      241 gcgaggaagt cggcctcgaa accgaacatg tagccctgcc ggaaaaaccg gtgctcgtgt
      301 ccgtcggctg gaaaaacctg aaaaaagtgt gggtcaaagt cgtgcaaaca ccgaacgatc
      361 cgacggtttt cgatcaggtg gaatgggacc aacgcctggc gttcctgaac aaactgcccg
      421 ccggcatcat tcatttccca atagcccacc aggcccggcg tgttgcccgg aatgctcacg
      481 cccatgttcg cgacgatctc agcggcgctg cgggcttcgt tccagacccg cagttgggcg
      541 atgttcccgt tgaacggttg cggatcaggt gcatcattac cgatgagcat gctggtggct
      601 gtggtagtgg tgctcggcgc accttgcagc accttcgagc cgatgtgcat gccatccgcg
      661 tagaaccgca ggctgtcggc gctcttcgtt acggcgatgt ggtggcaggt cccgtccatc
      721 aggctgccgt tgtagctgcc gttattgatc aggatgtagt tgaggccatc caactgcatg
      781 ttgatcatct tgtagttgga tccgccccag acaccgtgga agccgaatag aaagccgttg
      841 ttgacctgat cccggttgga gagaatgcgc ggatgctgca actggtcggc ctccacggcc
      901 ctgatctccg cctccaccgt gaagtcgccg gtgccgatcg cgttcaccgc agtgctggac
      961 aatgtgacct gatcatcggc gccatcgaag accaggcatc gccccatact cgtgaccgga
     1021 cagccatcca tgttccatgc ggggtcctcg tcctccacca cttcattggt acccagaagt
     1081 ccattcgtgg tgccggtctt gtcggcgatg tcctggccgg acccttcgtt caattcccaa
     1141 taacccacca gccccggcgt ggtaccggca atgctttgat ccttgttgga aaggatctcc
     1201 gccggtgagc ggacatgatc ccataaccgc agttgggaga gatggccgtt gaacggatgg
     1261 ggatccggcc cgtcgttccc gatgaccatg ttcgctgccg tggtggccac gctcgggttc
     1321 ccttgcagca ccttcgagcc gatgtgcgca ccgtccacat agaaccgcag gctgtccacg
     1381 cccttgctta ccgcgatgtg atggcaagtg ccatccagca cgctggcgtt gtacgtgccg
     1441 ttgttgatca ggatgtagtt gaggccatcc aactgcaggt tgaccatctt gtggttggat
     1501 ccaccccaga ggccatggaa ggcgaacatg aacccgttgt tgaccgcatc ccggttggag
     1561 aggatcctgg gatggctcgg ttgatcggct tcgacgccgc gcacccatgc ttccaccgtg
     1621 aacggccccg tgccgatact gttcagcacc gcgttgggca cggtcacctg gtcgttctgc
     1681 ccgtcaaaga cgagtcccgg atattgggcg gtggcgggga ggctacagag ggcgagaaat
     1741 ggcagtgtgt acgatcggat catggatcag gggttgttca cgtatagacg tagcacgcgc
     1801 ggatggttgt gtccgaagaa gaattttcga gtggaccgta gggtcgatcc tctgtcgaac
     1861 ggatcaaaca atggcgtcct tctgctcatc cgtccgccgg tcgcgcttct tgaaatcctc
     1921 cgccttcgtg ccgaaggcca gcgccaatac cagcgccgtg gagcagcctg cggtcacctt
     1981 ctgcatcact gccagcaggc cccagtcgcc catgccatag aggaacaagg tgtagtagaa
     2041 gatcagcatg caggccaccg cgagcacctt gagccagtgc tgcttggact ggaacagaag
     2101 cacggtgatg cagaacagcc caagtagcga gagcgtgctg gaggtggtga ccgccgcatc
     2161 atgcagcgtg gtggcgatga ggaagttgga cccgatgctc aggtggccca ccacccggag
     2221 catcggtctg atgacgcgct cggtgatcac gcgcgacatg cggatgaaga agaggccatc
     2281 gcccagggag tggaaggcca tgcccaccag cgcccacggc ctgccgggat tgtccgcacc
     2341 gttgagggcc ttctcctgga agagattgct gaagaagttc ttcgaccaga tgaagcccgc
     2401 gctattggca tcggcgatgg aaccgccggg gtatagcgag gcggcgatga gcatcaacgg
     2461 cactgtaaga agcagacagg ccaggatggc ccactgcaag cggcggcggc cagacgaaag
     2521 agaaggactt ccttcgtcca tggtgtttat tgttcgttgg tggaccggcg gtactgtaac
     2581 cgagcgcccg atccgcaggt gaagaacccc acggccaggg aattaccgcc gtgaaaggga
     2641 tgggtgggca aagaaccgat acggtgaata cgccctacac gtacattggc ttgaccggca
     2701 ggcagctacg aacaacttcc gtccttcgca gtacatcagg agtatatttc ctccgcaatt
     2761 ggaccgttac gaacttctcc tgcgcccggt ggcgcatatg catccagcgc agatcctcgc
     2821 cggtctttca accagcgatg tggtgcgccg tgtccccggc gtggcccaca acatcgtgga
     2881 gatcacggcc cacatgacct tctggcagga ttggttcctg gcgcgatgct ccggtgaagc
     2941 cttgcctctc gttatccatg ccacagaggg ttggccttcc gttggacaaa aggactggga
     3001 gcttgtgcac catgcgtttc tgaatggcct tgaccgcgcg gtgcatcatc cgggcagcgg
     3061 caagctgaag ccgccgatcg agctcgggtt gctcgcgcac tacgaggtgg aagatgtgat
     3121 ggcacacctt gcccaacaca acagccacca cctggggcag atcgtggtcc tgcgtcaagc
     3181 cttcggcctt tggcccccac ctggcggcag cttcacttgg tagcgtgggt gtccgttcca
     3241 cctgggatca tacggcgatt tgcctcaacg caccgaggat aatgctgtgt gtgcgcgtgt
     3301 tcacgcatgc cgcaacgcct tcaccggatc cgtctgcgcc gcacggatcg cccggaagct
     3361 caccgtaaac gtggcgatca acagcaccac gagcgcagca agcacgaaga ccagcggcgc
     3421 gcggcgggtg atgttcgcac tgctgggcgt
//
LOCUS       DAOQOC010000173         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13361_length_3449_cov_3.741897, whole genome shotgun sequence.
ACCESSION   DAOQOC010000173 DAOQOC010000000
VERSION     DAOQOC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13361_length_3449_cov_3.741897"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1459)
                     /locus_tag="PL106_03840"
     CDS             complement(203..1459)
                     /locus_tag="PL106_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA32232.1"
                     /translation="MSLSLQQAVDLAAKQGYAVQASALEAEKTLARTKEITAVGLPQI
                     SGSGTLNNYLKVPKMVVPNFFGGEGVAAIEFALPWSITGAVQLQQLLFDGSYLVGLQA
                     AKELRTKSAMDLEQTVLNAKVQATKAYLSVLAAEEGERITAEGLPLLEKALADAQVML
                     EQGLLETTDADRITVQLDETRNQQRNLHQQSSVARSYLALVLGLPAGTPISLTDALQP
                     LIDDPAWAGLADATFDANGHIDQKVAQSALRLAAMDVRNKKAAYLPSLGGFINYQQAY
                     NYIEFDIGNGSWWFPGSMWGLQLNVPIFSSGRRKNLVKQAEFAMEQAAVNVKATEQRL
                     LAEREQQQAVLRAAQDGYDTGKKNLALGRSIFERTSIKFNEGMASSFELTQEHGNYLT
                     AQQNHIQRIIALLQARADMRKALGAY"
     gene            complement(1522..2193)
                     /locus_tag="PL106_03845"
     CDS             complement(1522..2193)
                     /locus_tag="PL106_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA32233.1"
                     /translation="MAPGKNIPHPASALAPVDDRWERIVDGACRIFWKLGIKSVTMDD
                     VATRLGISKKTLYQYVTCKDDLVGRVLKHQEQSYQCDIEAVRSVEKQNAIDELYAITT
                     KVAAHLKDMHPSVHFDLEKYHPEAFRTLVTKRRTAIFRNMSDNLERGIREGLYREDLN
                     VPMIATIYLARFDLMFEGKLVPPATFNVEDLHWEIFRYHVRGIASRKGLDHLEKKMNK
                     EHGTR"
     gene            2324..2734
                     /locus_tag="PL106_03850"
     CDS             2324..2734
                     /locus_tag="PL106_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNA32234.1"
                     /translation="MPHLRRVHRTEKASSSMPATIYHLATCSTCQRILGSIPNLGKFT
                     LREIKSAPITAEELDGMKELAGSYEALFSRRSMKYRPLGLHEKRLTEKDYRIYILQEY
                     TFLKRPVMVVGDAIHIGNTKAAVEGMIAAGKKLK"
BASE COUNT          628 a         1038 c         1063 g          720 t
ORIGIN      
        1 gtcgctgttg tcggtgccgc cgcatgccgc gaggatcaca ggaacaagtg tgaagagaag
       61 gatacgtttc atgcgtggtc agttgcggtg gggagttgag cggtagggga gctgatgcgg
      121 gtagcgggtg aggcagcgca tgtggtctgt tccacgcggg atcgggcggt tggtccggtt
      181 cctggtgtgc gtgcgtcctt ggtcaatacg ccccgagggc cttgcgcata tcggcccggg
      241 cctgcagcag ggcgatgatg cgttggatat gattctgctg tgcggtgagg taattgccgt
      301 gctcctgggt cagttcgaag ctgctggcca tcccctcatt gaacttgatg gaggtgcgct
      361 cgaagatgct cctgccgagt gcgaggttct tttttccggt atcataacca tcctgggcgg
      421 cgcggagcac ggcctgttgc tgctcgcgct cggcaaggag ccgttgttcg gtggccttca
      481 cgttgacagc ggcctgctcc atggcgaact cggcctgctt caccaggttc ttgcgccgac
      541 cgctgctgaa gatgggcacg ttcagttgaa ggccccacat cgagcccggg aaccaccagc
      601 tcccgttccc gatatcgaat tcgatgtaat tataagcttg ttggtagttg atgaagcccc
      661 cgagcgatgg caggtaggcg gccttcttgt tgcgcacgtc catagccgcc agtcgcaagg
      721 cgctctgcgc caccttctgg tctatgtgcc cgttggcatc gaaggtcgca tccgcgagtc
      781 cggcccacgc gggatcgtcg atcagcggtt gcagggcatc ggtgaggctg atcggggtgc
      841 cggcgggcag gccgagtacg agcgcgaggt aggagcgtgc cacgctcgac tgctgatgca
      901 ggttccgctg ctggttccgc gtctcgtcca actgaacggt gatgcggtcg gcatcggtgg
      961 tctccaacag gccttgttcc aacatgacct gcgcatcggc caaggccttt tccagcagtg
     1021 gcagaccttc cgccgtgatc cgctccccct cctcggccgc cagtacgctc aggtacgcct
     1081 tggtggcctg cactttggcg ttgagtaccg tttgctccag gtccatcgcg ctcttggtgc
     1141 gtagttcctt ggcagcctgc aggcccacga ggtagctgcc atcaaagagc agctgctgca
     1201 actggaccgc acccgtgatg ctccacggca gagcgaactc gatggccgcc acaccctctc
     1261 caccgaagaa gttgggcacc accatcttgg gcactttcag gtagttgttc aatgtaccgg
     1321 acccgctgat ctgcggcagt cccacggcgg tgatctcctt ggtgcgggcc agggtcttct
     1381 ccgcttcaag cgcgctggcc tgtacggcat atccctgctt ggcggccagg tcgaccgctt
     1441 gttgcagact caggctcacg ggaccctgcg cacatgcgcc gagtgttgcc gacaagaaaa
     1501 ggaaagtggt gatggtcgac ctcatcgggt gccgtgttcc ttgttcattt tcttctccag
     1561 gtgatccagg cccttcctgc tggcgatccc gcgcacatgg taccggaaga tctcccaatg
     1621 cagatcctcc acattgaacg tggcgggcgg caccaacttg ccctcgaaca tcagatcgaa
     1681 gcgcgccagg tagatggtgg cgatcatcgg aacgttgagg tcctcgcggt acaggccttc
     1741 ccggatgccc cgttccaagt tgtcggacat gttgcggaag atcgccgtac ggcgcttggt
     1801 caccagggtg cggaaggcct cggggtggta cttctccagg tcgaaatgga cgctggggtg
     1861 catatccttg aggtgcgcag ccaccttggt ggtgatggcg tacaactcat cgatggcgtt
     1921 ctgcttctcc accgaccgga ccgcctcgat atcgcactga taactctgct cctggtgctt
     1981 cagtacgcgg cccaccaggt cgtccttgca ggtgacgtac tggtagagcg tcttcttcga
     2041 aatgcccagg cgggtggcca catcgtccat ggtcacgctc ttgatgccga gcttccagaa
     2101 aatgcggcac gcaccgtcca cgatgcgctc ccaacggtcg tccacaggtg ccagcgcgga
     2161 tgcgggatgc ggtatgttct tgccgggggc catcgttcag ggagcgcaaa aatagatgcc
     2221 atcggaacac aggaaacatt atgtaaggaa aaatgtttcc aatgtgatga acgggaactt
     2281 gcagggatgg gtggcggggt caaaaatgat ccgaccgcgt atcatgcccc atcttcgccg
     2341 cgttcaccgc acggaaaagg cttcttccag catgcccgcg accatctacc acctggccac
     2401 ctgcagcacg tgccagcgca tcctcggctc cataccgaac cttgggaaat tcaccctgcg
     2461 cgagatcaag agtgctccga tcaccgcgga agaactggac gggatgaagg agttggccgg
     2521 cagctacgaa gcgttgttca gccgtcggtc catgaaatac cggccgctgg gcttgcacga
     2581 aaaacggctc accgagaagg actaccgcat atacatcctg caggagtata ccttcctgaa
     2641 gcgcccggtg atggtggtgg gtgatgcgat ccacatcggc aacacgaagg cggccgtgga
     2701 gggcatgatc gccgccggga agaagttgaa gtgaagcagg tgagcgcgaa cgccccgccg
     2761 atattccacg gggcattcat ttgatgcacc tccttgccga tcgcccagga tgatcacaag
     2821 ggcgtgtccc gacgagcccc tcgaaccgtg aagtccgccg ataccggctt gttgtagcgc
     2881 tcctcgcatc gccggagcgg ctacgcatag gcgcagtcgg cgggctcagc gtgacagtgg
     2941 ggtcatgggt gtccacgagc actgttctat tactccctat gaactgtcac gctgagcgag
     3001 cctgccctga caccggaagg gccgaagcgt catactcctg ttcccagtac tcatcccggc
     3061 tcctggcgct atcccgcgct acaagcgctg atggacgggc acttccgttc ttgggtcggg
     3121 tattcggacc tgacctgccc gcttgaccac gaatttttgc cctcgcggat cgccacgggt
     3181 atcccgatca ctggaaacag gaagcaggtg aacgcgaacg ccccgccgat atcccgcggg
     3241 gcgttcattg gatgcttctc catgccgatc gccctggatg atcacaaagg cgtgtcgcgg
     3301 cgagcccacc gaaccgtgaa gtccgccggc caagccctcc tcagggcaag cgtgctcacc
     3361 atggctcttg ggaagtgcgg taacggaaca ctcttcaaga tcccgtttcc tactgctgcg
     3421 ggatcgcctc cttcttcgtt gagcgcaac
//
LOCUS       DAOQOC010000174         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13386_length_3446_cov_3.679740, whole genome shotgun sequence.
ACCESSION   DAOQOC010000174 DAOQOC010000000
VERSION     DAOQOC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13386_length_3446_cov_3.679740"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PL106_03855"
     CDS             complement(<1..1094)
                     /locus_tag="PL106_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32235.1"
                     /translation="MKVRITVALLVGILAGREVRAQGTLGDTLLPKAIAFLEKNLNDL
                     RQVGTRAQEFTNAFTDESSRVACDICEQRDQPLATERYAFLVEDAYPDGFAFVHRIDQ
                     KHITMRKLDRAEYEWRIPLTKTIPVSGKSSATYLSKSFTVHHMVLVRYLDGRFSIRSI
                     EAEKAREAQLFVDISGVMGMGDPTFADQKDFAPEVAANTLGIGVSWYFNPFSRTNKAN
                     VWLKTGLRASMRTEKLTAEGLRYEQVGIIHTSALGAHDQPVDLAPTIDVGQHIYDLTE
                     TTRSTLLTIPLSISKRAAMGSKLEFALEAEVNFSYAVLRNTSGHYTMDQTGTNHRINS
                     ELMDATGGTILTYTSPAAAVVDAATGERID"
     gene            complement(1091..>2604)
                     /locus_tag="PL106_03860"
     CDS             complement(1091..>2604)
                     /locus_tag="PL106_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32236.1"
                     /translation="ATATFTVTVNQPPDQPAIDPSGPAQLCAGDDIVLTTTATSPLWS
                     TGETSSSISVSTAGAVWVTDSTAGCPVAASDTVQVNVTGVEVVTCPADLALCSNGAPH
                     VLAGALPEGGTYLLNGTSITTFDPDTLAGEYSIRYRYNSCSDSCEFTITVVVPPNAGS
                     DSSLTICSNGTAVDLGTLLGVHDPGGTWTDQGGVEFDGVYDPAARPSGTYTYTVSGNA
                     PCSAASASVEVTNNQAPPEPLVWGVGLHTACDGQWMDISLPGAEPALQYQWVCDSCTF
                     ESDPDTMATVQWHNASDAEANGTLGVTVLDQTTGCSSSFFANIGISPATASCPKGIVF
                     FEPHGLAVLDPTARWFQWGTLLGNVFDPVEGGTDQTLFDPGLIHACEDHPYVARTSID
                     GECWSTTIRCADSLSLARICPEPQMATVPELRVDPNPWAGGPLWIAPFRTGSDDPVLV
                     EILDLQGRIRSSARLPSSGYDLGSTLEGGLSRGPWVLRAWIDGRPQSIKLIVQ"
     assembly_gap    2605..2631
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2632..3130)
                     /locus_tag="PL106_03865"
     CDS             complement(<2632..3130)
                     /locus_tag="PL106_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32237.1"
                     /translation="MRHILTLLMIWIACSARGQCTGIVAPSDTSVCDGQTLEFILSEA
                     GADVDSLQWQEGVPGAWSDIAGATDDTLQVCPDTDIHYRCVLFCASDTIAADSAEVTV
                     VSPITVTIAGQGGPYCGTASDTLVASGADAYAWEPTLSVDPDTGPEVVATPGSTTTYT
                     VTGSTG"
     gene            complement(3143..>3446)
                     /locus_tag="PL106_03870"
     CDS             complement(3143..>3446)
                     /locus_tag="PL106_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32238.1"
                     /translation="IKCISPERTPPVEFNRPFVCGFDREKATSHPIANMPVMFEGVKY
                     RISIKPVIGTMGYFPIQGNRFGVVDEQGGRLVGKGAIQFTSDTSTCLFDVVFRAAQ"
BASE COUNT          621 a         1031 c         1084 g          683 t
ORIGIN      
        1 aagtcgatac gttccccggt ggcggcatcc actacagcgg cagcggggga ggtgtaggtc
       61 aggatcgtac cgcccgtggc atccatcaac tccgaattga tgcgatggtt ggtccccgtc
      121 tggtccatgg tgtagtggcc gctcgtgttc cgcagtacgg cgtagctgaa gttcacctcc
      181 gcctccaacg cgaattcgag tttgctgccc atggcggctc gcttgctgat gctgagcgga
      241 atggtgagca gggtggagcg ggtggtctcg gtgaggtcgt agatatgctg gcccacgtcg
      301 atggtggggg caaggtcgac cggttggtcg tgcgcaccga gggcgctggt atgaatgatc
      361 cccacctgtt catagcgcag cccttcggcg gtgagttttt ccgtacgcat ggaggcgcgc
      421 agcccggtct tcagccagac gttggcctta ttggtacggc tgaacgggtt gaaataccag
      481 ctgacaccga tacccagggt gttcgccgca acttccggcg cgaagtcctt ctggtcagcg
      541 aaggtcggat cacccatgcc cattacaccg gagatgtcca cgaagagttg cgcctcccgg
      601 gccttctccg cctctatgga gcggatggag aaccggccgt ccagatagcg caccaggacc
      661 atgtgatgca ccgtgaagga cttgctgagg taagtggcgg aactcttgcc agacaccggt
      721 attgttttgg tcagcgggat cctccattcg tattcggcgc ggtccagttt ccgcatcgtg
      781 atgtgcttct ggtcgatccg gtgcacgaac gcgaagccat ccggataggc gtcttccacc
      841 aggaacgcgt atcgttcggt cgcgagcggc tggtcgcgtt gctcgcagat gtcgcaggct
      901 acccgcgagg attcatcggt gaaggcattg gtgaattcct gcgcgcgcgt gcccacctgg
      961 cgcaggtcgt tcaggttctt ctccaggaag gcgatcgcct tgggcagcag tgtgtccccg
     1021 agtgttccct gtgcccggac ctcgcgaccg gccaaaatgc ccaccaggag ggcgaccgtg
     1081 atccggactt tcattgcacg atcagtttga tgctctgagg tcgcccatcg atccaggccc
     1141 gaagtaccca tggcccacgc gacaagccgc cttcgagcgt gctgcccagg tcatatccgg
     1201 acgatggcaa cctggcggag gatcggatgc gcccttgaag gtcaaggatc tccacgagca
     1261 ccggatcatc agatcccgtt cggaagggag cgatccacag cgggcctcca gcccacgggt
     1321 tcgggtccac gcgcaattcg ggtacggtgg ccatttgcgg ctccgggcag atgcgggcca
     1381 gcgacaggga gtcggcacag cggatcgtgg tgctccaaca ctcaccatcg atgctggtcc
     1441 gggccacgta cggatgatcc tcgcaggcat ggatgagacc cgggtcgaac aaggtctggt
     1501 ccgtcccacc ctccacggga tcgaacacgt tccccagcaa tgtgccccat tggaaccatc
     1561 gtgcggtcgg atccaggacc gccagcccgt gcggttcgaa gaaaacgatc cccttggggc
     1621 aggatgccgt tgcgggcgaa atgccgatgt tggcgaagaa tgagctcgaa caacccgttg
     1681 tttgatccag aacggtgaca cccagtgtcc cgttcgcctc cgcatcggac gcattgtgcc
     1741 attgcacggt ggccatggtg tccggatcgc tctcgaaggt gcagctgtcg cacacccatt
     1801 ggtactgcaa tgccggttcg gccccgggca ggctgatgtc catccattgc ccatcgcaag
     1861 cggtatggag gcctacgccc catacgagcg gttccggtgg tgcctggttg ttcgtcacct
     1921 ccacgctcgc gctggccgcg gaacaaggtg cgttcccgct gacggtatag gtatatgttc
     1981 cggaagggcg ggccgccgga tcatacacac catcgaactc gaccccgccc tggtcggtcc
     2041 acgtgcctcc gggatcatgc acgcccaaga gggtgcccag gtccaccgcc gtgccattgg
     2101 agcagatcgt gagggagctg tccgaacctg cgttgggagg aaccacgacc gtgatggtga
     2161 attcgcagga gtcggagcac gaattgtacc tgtacctgat gctgtattcc ccggccagcg
     2221 tgtccgggtc gaaagtggtg atcgatgtgc cgttcaggag gtaggtgcca ccttcgggca
     2281 gtgccccggc aagtacatgg ggtgcgccat tcgagcaaag cgccagatcg gcggggcagg
     2341 tcacgacctc cacacccgta acattgacct gtacggtatc actggccgca accgggcatc
     2401 cggccgtact gtccgtgacc caaacagcgc cggcggtgga caccgatatg gaggacgacg
     2461 tttcgcccgt gctccacagt ggtgatgtcg cggtagtggt cagcacaatg tcgtcacctg
     2521 cgcacaattg tgccggtccg gaaggatcga tggccggctg gtccggcggt tggttcaccg
     2581 tgaccgtgaa tgtggccgtg gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nggcctgtgc
     2641 tgccggttac cgtgtacgtg gtggtcgaac ctggcgtcgc caccacttcg gggcccgtgt
     2701 cggggtcgac gctgagcgtg ggttcccatg cgtacgcatc cgcaccgctg gcaacaagcg
     2761 tgtcactggc cgttccacaa tacggccctc cctggccggc gatggtcacc gtgatcggtg
     2821 aaacgaccgt cacttcggcc gagtcggcgg ctatggtatc gcttgcgcag aagagcacgc
     2881 aacggtagtg gatgtccgtg tccgggcata cctgcagcgt atcatccgtt gctccggcaa
     2941 tatccgacca tgcaccgggt acgccttcct gccattgcag gctgtccaca tcggctccgg
     3001 cttccgacag gatgaattcc aaagtctggc cgtcacagac cgaggtgtcc gatggtgcaa
     3061 cgataccggt acactgcccc cgcgccgaac aagcgatcca gatcatcagc aaggtcagga
     3121 tgtgtcgcat gtggctccac tgttactgtg cggcccggaa gaccacgtcg aagagacagg
     3181 tggacgtatc gctggtgaac tggatggcgc ctttccccac caggcgccct ccttgttcgt
     3241 ccactacgcc gaatcggttg ccttggatgg ggaaatagcc catggtcccg atgacaggtt
     3301 tgatactgat ccggtatttg accccttcga acatcaccgg catattggcg atcggatggg
     3361 aggtggcctt ctcccgatcg aaaccgcata cgaatggacg gttgaactcc accgggggtg
     3421 tccgctcagg ggatatgcac ttgatc
//
LOCUS       DAOQOC010000175         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13520_length_3424_cov_3.980706, whole genome shotgun sequence.
ACCESSION   DAOQOC010000175 DAOQOC010000000
VERSION     DAOQOC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13520_length_3424_cov_3.980706"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..636)
                     /locus_tag="PL106_03875"
     CDS             complement(79..636)
                     /locus_tag="PL106_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNA32239.1"
                     /translation="MRSTRAVQSGLEGYFRDPLFIRMPEHRILLPETRDRSLLYDALV
                     PQVRELLRDEPDPVAAMANMAAALRETFGWHWIGFYRVLGDELVLGPFQGPVACGRIP
                     FGKGVCGKAWAENRILTVDDVDQFPGHIACSALSRSEIVVPIHGRSGSVAAVLDIDSS
                     ALADFSEVDAHHLQQLSALIAPLLG"
     gene            complement(629..1324)
                     /locus_tag="PL106_03880"
     CDS             complement(629..1324)
                     /locus_tag="PL106_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNA32240.1"
                     /translation="MRPHGNLFHDLAGLFLPRRCSGCTTVLLRHEGAICGSCLADLPL
                     TRFHDDGHNPVEQVFLGRLRLEAASALLSFTRDGIVQHLLHRLKYRGDREAGLELGRL
                     MARDAMASRRFASVDTAIAVPLHPSKLRQRGYNQAQVLVDGMRELWPLRDPGRELLRI
                     ARTRTQTKKGRLERWANVKNAFQLTDPETLRDAHVLLVDDVVTTGATIEACTRALSSV
                     PGIRFSVFTCAYA"
     gene            1432..2337
                     /locus_tag="PL106_03885"
     CDS             1432..2337
                     /locus_tag="PL106_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32241.1"
                     /translation="MDQLTPAPKNRSNTLLLALVVLLLISNVVMLYLWNQKGDQAQQA
                     QAQVAAVSSEKENVTKLLEDMLVQYDTLSSENEQLNAEMAAQQKQIEELMKKVQSGNY
                     SLAKAKKEAETLRKIMQGYVVTIDSLNQLNQALTAENLSTKQELGEVKGQKAALETQS
                     AEQQAIIARGSVLRSTVMTAGGIFERSSGKQVDTDRAKKATQVKCCFTVGENLTARPG
                     NKTLYLRVISPDGSVLPAAGGDNRFQFNGVEGEYSAKRDIDYQNQPVEACIYWKAPGA
                     MASGQYVVEVYESGGQVGTTSFNLK"
     gene            2395..2748
                     /gene="ytxJ"
                     /locus_tag="PL106_03890"
     CDS             2395..2748
                     /gene="ytxJ"
                     /locus_tag="PL106_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNA32242.1"
                     /translation="MEWTPLVDVAQLDAIDQLSKNHPVLIFKHSTQCSISQGALGRMD
                     AGDPAAGAIGSPSFYLDLLAYRSVSNAVEARYGIRHESPQVLVIRDGRCVFHTSHRAI
                     TYVDTLAAMERAAVQ"
     gene            complement(2898..>3424)
                     /locus_tag="PL106_03895"
     CDS             complement(2898..>3424)
                     /locus_tag="PL106_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA32243.1"
                     /translation="KHGIEKIKTIGDAYMAAGGLPTPNTTHATDAIKAALEMRDFIAE
                     GKVRKVAAGLPYFEIRIGIHTGPVVAGIVGLKKFQYDIWGDTVNTASRMESSGEVGQV
                     NISEATYRLVKDAKRVNGEWSIANGGDSGSTAHSPFTNSHSPAFTFTPRGKVQAKGKG
                     EMEMYFVSRSLGEG"
BASE COUNT          687 a         1020 c         1118 g          599 t
ORIGIN      
        1 ccttggcccc gccggagagt tcgcgtgtct gcgcgcaggc ggcgaacagc aggatgagga
       61 aggccacgtg gaatcgcttc atcccaggag cggtgcgatc agtgcgctga gttgttgcag
      121 atggtgagcg tccacctcgc tgaagtcggc gagggcggag ctgtcgatgt cgagcaccgc
      181 ggccacgctg cccgagcgtc catggatggg caccacgatc tcgctccggc tgagcgcgct
      241 gcaggcgatg tgcccgggga attggtccac atcatcaacc gtaaggatcc tgttctcggc
      301 ccaggccttg ccgcaaacgc ctttgccgaa cgggatccgg ccgcatgcca caggaccctg
      361 gaagggcccc aggacgagtt catcgcccag cacgcggtag aagccgatcc aatgccaacc
      421 gaacgtttcg cggagcgcgg cggccatgtt ggccatggcg gccaccgggt cgggttcatc
      481 gcgcagcagt tcgcgcacct gcggaacgag cgcgtcgtag aggagcgagc gatccctggt
      541 ttccggaagc aggatacggt gctcgggcat gcggatgaac agcgggtcgc gaaaataccc
      601 ctccaggccg ctctgtacgg cgcgtgtgct acgcataggc gcaggtgaac acgctgaagc
      661 ggataccggg cacactggag agcgcccggg tacaggcttc gatggtggca ccggtggtca
      721 ccacatcatc caccaggagg acgtgggcat cgcgcagggt ttccgggtcg gtcagttgga
      781 acgcgttctt cacgttggcc catcgttcga ggcgtccttt tttcgtttgg gtgcgcgtgc
      841 gagcgatgcg gagcagttcg cgaccgggat cacgaagagg ccacagttcg cgcatgccat
      901 ccaccagcac ctgggcctga ttgtacccgc gctgacggag cttggaggga tgcaggggca
      961 cggcgatggc ggtatcaacg ctggcgaagc gtcgggatgc catggcgtcg cgggccatca
     1021 gtcgaccgag ttccaggcca gcttcgcgat caccccggta cttcaatcgg tgcagcagat
     1081 gttgtacgat gccatcacgc gtgaaactca acaatgcgct ggcggcctcc agtcgcaacc
     1141 ggccgaggaa gacctgttcc accggattgt gcccatcgtc atggaagcgc gtgaggggaa
     1201 ggtcggccag gcaggacccg cagatggccc cctcgtgccg caacagcacc gtggtgcaac
     1261 ccgagcagcg gcggggcagg aatagcccgg ccagatcgtg gaacaggttg ccgtgggggc
     1321 gcatgcttcc caataacaat gttgataacc gcgttgaacc ccgaaagtcc tttcccaagg
     1381 ccaaggtaat ttcgcagggc cattgttccg catagtggcc aaaattccaa catggaccaa
     1441 ctgacccccg ctccaaagaa ccgctccaac acactgctgc tcgccctggt ggtgctgttg
     1501 ctgatcagca acgtggtcat gctctacctg tggaaccaga aaggcgacca ggcccagcag
     1561 gcccaagcgc aggtggcggc tgtttcctcc gagaaggaga acgtgaccaa attgctggag
     1621 gacatgctgg tacagtacga cacgctcagt tcggagaacg agcagttgaa cgccgagatg
     1681 gccgcccagc agaagcagat cgaggaattg atgaagaagg tgcagtcggg caactacagc
     1741 ctggccaagg cgaagaagga agcggaaacg ctgcggaaga tcatgcaggg gtatgtggtc
     1801 accatcgatt cgctcaacca gttgaaccag gcccttacgg cggagaacct cagcaccaag
     1861 caggagttgg gcgaggtgaa agggcaaaag gccgcactgg aaacgcagag cgccgagcag
     1921 caggcgatca ttgcgcgggg atcggtgttg cgcagcaccg tgatgacggc cggtggcatc
     1981 ttcgagcgca gcagcggcaa gcaggtggac acggaccggg ccaagaaggc cacccaggtg
     2041 aagtgttgct tcaccgtggg cgagaacctc accgctcggc ctggcaacaa gacgctctac
     2101 ctgcgggtga tcagccccga tgggtcggtg ctgcccgctg cgggcggcga caaccggttc
     2161 cagttcaatg gggttgaggg tgagtacagt gccaaacgcg acatcgatta ccagaaccag
     2221 ccggtggagg cctgcatcta ttggaaagcc cccggggcga tggccagcgg tcagtacgtg
     2281 gtggaggtgt acgagagcgg cggacaggtg ggcaccactt cgttcaacct gaagtagccg
     2341 ggcaaggcgg tccgagcgcc gggcccccaa caggtcggaa gccgacttca cgccatggaa
     2401 tggacacccc ttgtggacgt cgcacagctc gacgccatcg atcagctatc gaagaaccat
     2461 ccggtactga tcttcaagca cagcacgcaa tgcagcatca gccagggagc gctgggccgc
     2521 atggatgcag gggaccctgc tgccggggcg atcggatccc cgtccttcta cctcgacctg
     2581 ctggcctacc gatcggtaag taatgcggtg gaagcacgat acggcatccg gcacgagtca
     2641 ccgcaggtgc tggtgatccg ggacggacgc tgtgtgttcc acacctcgca ccgggccatc
     2701 acgtacgttg atacgttggc ggcgatggag cgggcggcgg tacagtgacc ttgtaggtcg
     2761 tacggagcac gccggacgcg gatcaatgga cctgctcgga cagggcatcg ggcatttacc
     2821 ggctcgaagc gggagcactg cgagaacctc aagcgctggc accgggccat tgcgcgctgc
     2881 cgaccggatg aacggaatca gccttcgccg aggcttcggc taacgaagta catctccatt
     2941 tcccctttgc ctttggcttg cactttcccg cgcggggtga aggtgaacgc gggtgaatgg
     3001 gagttggtga atggtgaatg ggctgtgctt ccgctatctc caccattcgc tatcgaccat
     3061 tcgccattca ccctcttcgc gtccttcacc aaccggtacg tggcttcgct gatgttcacc
     3121 tgtccaacct caccgctgct ctccatgcgg ctggcggtgt tcaccgtatc accccagata
     3181 tcatactgga atttcttcag accaacgatc ccggcgacca cggggccggt gtggatgccg
     3241 atcctgatct cgaagtaggg cagtccggcc gcgactttcc gcaccttgcc ctcggcgatg
     3301 aagtcgcgca tctcgagggc cgccttgatc gcgtcggtgg catgggtggt gttgggtgtg
     3361 ggcaagccac cggcggccat gtacgcatca ccgatggtct tgatcttctc gatgccgtgc
     3421 ttat
//
LOCUS       DAOQOC010000176         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13559_length_3418_cov_4.231044, whole genome shotgun sequence.
ACCESSION   DAOQOC010000176 DAOQOC010000000
VERSION     DAOQOC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13559_length_3418_cov_4.231044"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="PL106_03900"
     CDS             complement(<1..1633)
                     /locus_tag="PL106_03900"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32244.1"
                     /translation="MVAGIRSFFCATLITLVSPVSAGPASTTTDSLKVLLARSAPDTT
                     RVNLCTRLLRSLIGSDPQQALAFGTAAVHLADSLHWDPGRRNARQALARAYWSSGDFK
                     QAERLDMEVLHMMEHDAEPRAMATQLQYLGQDHLDGGNTAEAHKYLGRSLDLWRSIND
                     SSAMANLLGLFSYLHELDGDLTASAKATHEALAIYEALDKSSGIAMSNANLAHTYQAL
                     GRLDEAMEFYLRALRTYDARTASDSINLAIAHSELGDLYLRMGMPDKARTHFGNALEI
                     GTRIQDQGQMALANRGFGAIAMQQKDYEHALPALLLAAEQFEASGFMRIDEARTHTDI
                     GYCQAHLGDMEAAARTFAHVRAIADSMNSPLILAGYFKSLEFLDSLRGDWEGAYRNGH
                     RYRMIQDSIAGRASQEQVVKAQLRYEEEKRELQRRAAQEQRDMHQRTIRNSIIAVLAG
                     ALVFLAVVYRQRNKINRARKRSDELLLNILPEEVAEELKLKGEAEAVHIDQVTVLFTD
                     FKGFTAVSEVVTPRQLVHDLHECFSAFDRICEKHGI"
     gene            1779..1904
                     /locus_tag="PL106_03905"
     CDS             1779..1904
                     /locus_tag="PL106_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32245.1"
                     /translation="MSIIDIAVLLCPRNIVNVMMGPCINTGDHSIGAVRACGYCS"
     gene            1949..2377
                     /locus_tag="PL106_03910"
     CDS             1949..2377
                     /locus_tag="PL106_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32246.1"
                     /translation="MYTGLKHFHSYFAYLVLLMVAVAAINAFLSRSSGKPFAGTGRKL
                     ALFGLIAAHLQLLFGLLLYFVSPMGFSNFSGENMGNATSRLYMLEHPLMMIIGITLIT
                     MGWSKAKRAADDGKKYGAIALWYGIGLVLMLSRIPWSVWP"
     gene            complement(2378..2701)
                     /locus_tag="PL106_03915"
     CDS             complement(2378..2701)
                     /locus_tag="PL106_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA32247.1"
                     /translation="MGKAAISQGKLTTPEAAECPAEQALRSISGKWKPRILRAASEAP
                     VRFNQLLRDLPESNKQALTNALRELESDGLLVRSVVRQKPLHVEYMLTDQARTIIGLL
                     RAMEP"
     gene            2890..>3418
                     /gene="ygiD"
                     /locus_tag="PL106_03920"
     CDS             2890..>3418
                     /gene="ygiD"
                     /locus_tag="PL106_03920"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNA32248.1"
                     /translation="MKLNDLATLGAQFHRSERMPVLFLGHGSPMNAIEENEFVAGFRT
                     IGSTLPRPQAILVVSAHWETRGTLVTAMEHPRTIHDFGGFPQALFDVQYPAPGSPALA
                     EEVKRTITGTPVGLDHGWGLDHGAWSVVKHLYPNADVPVIQMSIDRTITPEKHYALGK
                     ELAALREKGVLIIGSG"
BASE COUNT          599 a         1081 c         1042 g          696 t
ORIGIN      
        1 cgatgccgtg cttttcgcag atgcggtcga aggcactgaa gcattcgtga aggtcgtgta
       61 ccaactggcg cggcgtcacc acctcgctca cggccgtgaa tcccttgaag tcggtgaaga
      121 ggacggtgac ctgatcgata tgcacggctt cggcttcacc tttcagtttc agttcctcgg
      181 cgacttcctc cggcaggatg ttgagcagca gttcatcgct gcgcttgcgg gcccggttga
      241 tcttgttgcg ctgccggtac accacggcca ggaagacgag tgctccggca agcacggcga
      301 tgatggaatt gcggatggta cgctggtgca tgtcgcgttg ttcctgcgcc gcgcgccgtt
      361 gcaattcgcg cttctcctcc tcgtagcgca actgggcctt caccacctgc tcctggctgg
      421 cccgtccagc gatgctgtcc tggatcatgc gatagcgatg cccgttccgg taggcgccct
      481 cccagtcgcc gcgcagactg tccaggaact ccaggctctt gaagtatccc gcgaggatca
      541 gcggactgtt catgctgtcg gcgatggcgc gcacatgtgc gaatgtccga gccgcggcct
      601 ccatatcccc gaggtgggcc tggcagtaac cgatatcggt gtgggtgcgg gcctcatcga
      661 tcctcatgaa tcccgacgcc tcgaactgtt cggccgcaag aagcagggcg ggcagggcgt
      721 gctcgtagtc cttctgctgc atggcgatgg caccgaagcc gcgattggcc agcgccatct
      781 ggccctggtc ctggatccgg gtgccgatct ccagtgcgtt cccgaagtgg gtgcgcgctt
      841 tgtccggcat acccatgcgc aggtacaggt cgccgagttc gctgtgggcg atggccaggt
      901 tgatggaatc ggaggccgtg cgggcatcgt atgtgcgcaa ggcgcgcagg tagaattcca
      961 tcgcttcatc aagccggccc agggcctggt acgtgtgcgc gaggttggca ttgctcatgg
     1021 cgatgccact gctcttgtcc agggcctcgt atatggccag agcttcgtgg gtggccttgg
     1081 ccgaagcggt gagatccccg tccagttcgt gcaaatagga gaagagcccg agcaggttgg
     1141 ccatggcgga actgtcgttg atcgatcgcc acagatccaa ggaccgcccc aggtatttgt
     1201 gcgcttccgc cgtattgccc ccgtccaaat gatcctgtcc gaggtattgc aattgcgtgg
     1261 ccatggccct gggttcagcg tcgtgctcca tcatgtgcag cacctccatg tccaaacgct
     1321 ccgcctgctt gaaatcgccc gatgaccagt acgccctggc cagcgcctgc ctcgcgttcc
     1381 gccgcccggg atcccaatgc aacgaatcgg ccaggtggac tgcggctgtc ccgaacgcaa
     1441 gtgcctgctg cgggtccgaa ccgatcaggg agcggagcag tcgtgtgcag aggttcacgc
     1501 gtgtggtatc cggcgcgctc cgggccagca gcaccttcag ggagtccgtc gtggttgatg
     1561 ccgggccagc ggataccggc gatacaagcg tgatcaatgt cgcacagaag aacgatcgga
     1621 tcccggcgac catgcgatca aagtacactg tttttgtaca tcgctgtgcg tcgggcaggt
     1681 gcgctccctc cggatccctg gccgaccagg atcctgtccg gccgatgggt ggaccggtcg
     1741 gtaggggaga agtacctcga tccattgggc gcaggatcat gtccatcatc gacatcgccg
     1801 tcctgctatg tccccggaac atcgtcaacg tcatgatggg tccctgcatc aacaccggcg
     1861 accattccat cggagcggtc cgcgcatgcg gctattgttc gtgacctgca cctgtgccgt
     1921 agtttcgcgg accgctcaaa taccgaccat gtacaccgga ctcaagcact tccattcgta
     1981 cttcgcctac ctcgtgctgc tgatggtggc cgtggccgcg atcaacgcct tcctgtcccg
     2041 ctcctccgga aaacccttcg ccggcacggg ccgcaagctg gcgctcttcg ggctgatcgc
     2101 ggcacatctg caattgctct tcggcctctt gctctacttc gtgtcgccca tgggcttcag
     2161 caacttctcc ggcgagaaca tgggcaacgc cacgtcgagg ctgtacatgc tggaacaccc
     2221 gctgatgatg atcattggca tcacactgat caccatgggc tggtccaagg ccaaacgtgc
     2281 agcggatgat gggaagaagt acggagcgat cgccttgtgg tacggtatcg gactggtgct
     2341 gatgctgagt cgcatcccat ggagcgtgtg gccctgatca gggttccatc gcacgcagca
     2401 aaccgatgat ggtgcgggcc tggtccgtca gcatgtattc cacatgcaac ggcttctgcc
     2461 gtaccacaga acgcacaagc aggccgtcgg actcgagttc acgcagcgca ttggtgaggg
     2521 cttgtttgtt gctctccggc aggtcgcgca gcaattgatt gaatcggacc ggtgcctctg
     2581 acgccgcacg caggatgcgg ggtttccact tgccgctgat gctgcgcaaa gcctgttcgg
     2641 ccgggcattc cgccgcctcc ggggtggtca acttcccttg acttattgca gccttgccca
     2701 tgtgtgagga cctttgtgcc gcaagttacc catgcacatg ccgccccccg atcgccattg
     2761 tgttcacccg gtcccgacca ggcatccata caaggaccgt tcggcaccga tcacgatcca
     2821 cggatgagcc gcatggaccg caaacagttc ctcctctccc tcaccgccct cccgttgacc
     2881 ggcatggcca tgaaactgaa tgacctggcg accctcggtg cgcaattcca ccgtagcgaa
     2941 cgcatgcctg tgctcttcct gggccatggc agtccgatga acgcgatcga ggagaacgag
     3001 ttcgttgcgg gattccgcac catcgggtcc acgcttccca ggccccaggc catcctggtg
     3061 gtgagcgccc actgggaaac acgcggcacg ctggtcaccg ccatggagca tccgcgcacg
     3121 atccacgatt tcggtggatt cccgcaggcc ctcttcgatg tgcaataccc ggcaccgggt
     3181 agtccggccc tggcggagga ggtcaagcgg accatcaccg gcacgccggt cgggcttgat
     3241 cacggctggg gcctcgatca cggtgcttgg agcgtggtga agcacctcta tccgaacgcc
     3301 gacgtgcccg tgatccagat gagcatcgat cgcaccatca caccggagaa acactacgcg
     3361 ctcgggaagg agctcgctgc gctgcgcgag aagggtgtgc tcatcatcgg cagcggca
//
LOCUS       DAOQOC010000177         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13659_length_3403_cov_3.527180, whole genome shotgun sequence.
ACCESSION   DAOQOC010000177 DAOQOC010000000
VERSION     DAOQOC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13659_length_3403_cov_3.527180"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PL106_03925"
     CDS             complement(<1..705)
                     /locus_tag="PL106_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNA32249.1"
                     /translation="MRHSVAHFLLGIALIVGCRTSAPVANAPPPSPAGGPLSWEQTLA
                     QQNTDATIYQHSSAEVYRLYQQCYELARIRLDANLALPRNLPPAVVVDIDETVLDNSP
                     YNVKAAWQGHAFDPKTWNEWVLMASAKALPGALEFLRYASKQGCTVFYITNRNEAERN
                     ATIKNLFELGFPDADEAHVMCMDGSGSDKTKRRGRVMGTHTIVLLVGDQLRDFDEMYK
                     DRSIDMGRPTVDANRDM"
     gene            complement(749..1699)
                     /gene="rsgA"
                     /locus_tag="PL106_03930"
     CDS             complement(749..1699)
                     /gene="rsgA"
                     /locus_tag="PL106_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNA32250.1"
                     /translation="MPIGLVMRSTGSRYLVRGADGVRHMCVAKGNLRIKGWKSTNPVA
                     VGDRVEFTPAAETIGNELVGAITGLHDRSNYIVRRSVNLSHHKHVIAANLDQALLLVT
                     MARPRTSHGFIDRFLVTAEAYRVPCIIIFNKVDDHGAVELELLAEYQNAYHLAGYRTI
                     ATSALTGTGIEEVKNALAGKVTLVAGHSGVGKSTLINAIAPDLDLPTFEVSEASEKGQ
                     HTTTYAEMFELDATTFIIDTPGIKGFRLVDMEAGEIVDQFPEMFRLKNDCRFNDCKHL
                     DEPGCAVKAAVENGTIAASRYISYVDMLNGVEDESPYRTD"
     gene            1863..2771
                     /gene="gldA"
                     /locus_tag="PL106_03935"
     CDS             1863..2771
                     /gene="gldA"
                     /locus_tag="PL106_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNA32251.1"
                     /translation="MSISVRQVSKLYGAQKALDEVSFEIGGGEVVGFLGPNGAGKSTM
                     MKVLTCYIPQSAGEASVCGFDVREKSMDVRRQVGYLPEHNPLYLDMYVREYLEFAADI
                     FKLKNRAQRVKEMILAVGLEREAHKKIGQLSRGYRQRVGLAQAMIHDPKVLILDEPTS
                     GLDPNQLEEIRALIKNLGREKTVMLSTHIMQEVEAICTRVIIIDKGRIVADDRAERLR
                     GTGSALEAVLVEFDSDVAPNELRNITGVVQVSKGPERAWVLGYDRVNDVRPAIFEFAV
                     KKGVKVLGMQKAERGLEEVFKELTKH"
     gene            2973..>3403
                     /locus_tag="PL106_03940"
     CDS             2973..>3403
                     /locus_tag="PL106_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNA32252.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDHFLAFDPSSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQQIAREVIDRIGYTKSEYMFEAHSCGVLSAIHEQSPDI
                     NQGVERKKPEEQGAGDQGMMFGYACRDTDNYMPLPLEISHL"
BASE COUNT          627 a          967 c         1151 g          658 t
ORIGIN      
        1 catgtccctg ttcgcgtcga ccgttgggcg gcccatgtcg atggagcggt ccttgtacat
       61 ttcgtcgaag tcgcgcaatt gatccccgac aagcaggacg atcgtgtggg tgcccatcac
      121 cctgccgcgg cgcttggtct tgtccgatcc cgagccgtcc atgcacatca cgtgggcctc
      181 gtcggcatcg ggaaagccca gctcgaagag gttcttgatg gtggcgttcc gttccgcttc
      241 gttgcggttg gtgatgtaga agacggtgca gccctgcttg ctggcgtacc gcaggaattc
      301 caacgcgccg ggcagggcct tggcgctggc catcagcacc cattcgttcc aggtcttcgg
      361 gtcgaacgcg tggccctgcc aggcggcttt cacattgtag ggcgagttgt caagcaccgt
      421 ctcgtcgatg tccaccacca cagcgggcgg caggttgcgc gggagcgcga ggttggcatc
      481 caggcggata cgtgccaact cgtagcattg ttggtagagc ctgtacactt cggcactgct
      541 gtgctggtag atggtggcgt cggtgttctg ttgggccagg gtctgctccc acgacagagg
      601 gccaccggcg ggcgatgggg gtggcgcgtt ggcaacagga gccgatgtgc ggcagccgac
      661 gatcagtgcg atgccgagga ggaagtgcgc aacggagtgt ctcatgggtg ttgggcaggg
      721 tcgttcggtt ggtggtggtc ggccgaggtc aatcggtgcg gtaggggctt tcatcctcca
      781 cgccgttgag catgtccacg tagctgatgt agcggctcgc ggcgatggtg ccgttctcca
      841 cggcggcctt taccgcgcaa ccgggctcat ccaagtgctt gcagtcgttg aagcggcagt
      901 cgttcttcag ccggaacatt tcggggaact ggtccacgat ctcgcccgcc tccatgtcca
      961 ccagcctgaa acctttgatc ccaggggtat cgatgatgaa ggtggtggca tcgagttcga
     1021 acatttcggc gtatgtggtg gtgtgctgcc ctttctcact ggcttcgctc acctcgaagg
     1081 tgggcaggtc caggtccggg gcgatggcgt tgatcaacgt gctcttgccc acgccgctgt
     1141 gcccggccac cagggtcacc ttgcccgcga gggcgttctt cacctcctcg atccccgtcc
     1201 cggtcagtgc ggaagtggcg atggtgcggt agcccgcgag gtggtacgca ttctgatact
     1261 cggcgagcag ttccaattca accgcgccgt ggtcatccac cttgttgaag atgatgatac
     1321 agggtacgcg gtaggcttcg gcggtgacga ggaagcggtc gatgaaacca tgggaggtgc
     1381 gcggccgggc catggtgacg agcaacagtg cctggtccag attggcggcg atcacgtgct
     1441 tgtggtggct caggttcacg ctgcgccgca cgatgtagtt ggaccgatcg tgcaatccgg
     1501 tgatggcgcc caccagctca ttgccgatgg tctccgccgc cggggtgaat tccacccgat
     1561 cccccacggc tacgggattg gtgcttttcc agcccttgat gcgcaggttg cccttggcca
     1621 cgcacatatg gcgcacgccg tccgcaccgc gcacgaggta gcggctgccg gtggaacgca
     1681 tcacgagacc gatgggcatg ctgtgctcgc cgaagcttcc gcgcaggcga tgggacaaag
     1741 atgggaggtt catctgccca gggatccctg tgcgcatgat cgatcggcga tcacaaccac
     1801 ctgtgccgaa cgggcggtgg ccctggatga cgtgcggacc gcgcgcatgc tacattcgcc
     1861 gcatgtccat ttcggtccgt caagtgagca agctgtacgg cgctcagaag gcgctcgacg
     1921 aggtgtcgtt cgagatcggt ggaggggagg tggtgggatt cctgggtccc aatggtgcgg
     1981 gcaagagtac gatgatgaaa gtactcacct gctacatccc gcagagcgcg ggcgaggcca
     2041 gcgtgtgcgg cttcgatgtg cgggagaaga gcatggacgt acgtcggcag gtgggctacc
     2101 tgcccgagca caacccgctt tacctggaca tgtacgtgcg ggagtatctt gaattcgcag
     2161 cggatatctt caagctgaag aacagggcgc aacgtgtcaa ggaaatgatc ctggcggtgg
     2221 gcctggagcg ggaggcgcac aagaagatcg gtcagttgag caggggctat cgccagcgcg
     2281 tgggcctggc ccaggccatg atccatgacc ccaaggtgtt gatcctggat gaacccacga
     2341 gcggactcga tccgaaccag ttggaggaga tccgtgcgct gatcaagaac ttgggacggg
     2401 agaaaacggt gatgctgagc acgcacatca tgcaagaggt ggaggccatc tgtacccggg
     2461 tgatcatcat cgacaagggg cgcatcgtgg ccgacgacag ggcggaacga ttgcggggca
     2521 ccggatccgc attggaggcg gtgctggtgg agttcgactc ggatgttgcg cccaacgaac
     2581 tgcggaacat cactggtgtg gtgcaggtga gcaagggtcc ggagcgtgcc tgggtgctgg
     2641 gctacgaccg cgtgaacgat gttcgccccg cgatcttcga gttcgctgtg aagaagggcg
     2701 tgaaggtgct ggggatgcaa aaagcggagc gggggctgga agaagtgttc aaggaactga
     2761 ccaagcactg agcgtcaaga tccttgctga ccctgcgagt ggcgcttgtt cggcagggac
     2821 ctgccggtcg ggaggacggg tccggcttgc acacggtcct tcaggggtgt tggccctgcc
     2881 tttccggtgt gatcgaccgt tggaaagtcg ttggcaaaac cggtgatccc cccgggcaaa
     2941 accccttcct ttgccgccgc gaaaaaagaa ccatgccata cctcttcacc tccgagtccg
     3001 tcagcgaagg ccaccccgac aaggtcgccg accagatcag tgatgccctc atcgaccatt
     3061 tcctcgcctt cgaccccagc agcaaggtgg cctgtgaaac actggtgacc accggccagg
     3121 ttgtgcttgc gggggaggtc aagagcaagg cctacctcga cgtgcaacag atcgcgcgcg
     3181 aggtgatcga ccgcatcggg tacaccaaga gcgagtacat gttcgaggcg cacagctgcg
     3241 gcgtgctcag cgccatccac gagcagagcc ccgacatcaa ccagggtgtg gagcgcaaga
     3301 agcccgagga gcaaggtgct ggcgaccaag gcatgatgtt cggctacgcc tgccgcgaca
     3361 ccgacaacta catgccgctg ccgttggaga tcagccacct gtt
//
LOCUS       DAOQOC010000178         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13687_length_3399_cov_3.211722, whole genome shotgun sequence.
ACCESSION   DAOQOC010000178 DAOQOC010000000
VERSION     DAOQOC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13687_length_3399_cov_3.211722"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PL106_03945"
     CDS             complement(<1..539)
                     /locus_tag="PL106_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32253.1"
                     /translation="MKNAVLLLASAMLVGCRHDPPVEPEPLPPPGGTPSGVLRITFRP
                     IWDGTAFDKTNVYLSAANERVLIQQVKFYLSPLVFSGDTTARATDAVLLDLVNGPEAR
                     SYSLPVGTYDSLHYGLGLPYDLNHVDVTGIDPSSDLGAGSGMYWTWATMYRFLIFEGR
                     FDTLSTGIGTPPYQFSIHTG"
     gene            complement(540..1295)
                     /locus_tag="PL106_03950"
     CDS             complement(540..1295)
                     /locus_tag="PL106_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32254.1"
                     /translation="MRKTILALFSTAVLLAGCKKDDLEETHENLVGPDGANTVVAMSM
                     SFTHNGAAFDTSMVLTDVQGVRYKLEQLRFYMGHFDFTNDNHDSVASFPNKYLKVDLA
                     EGGTIRNIGQLSGHLHDMSFGLGVDSVNNHADPATVEAPLGVNGMYWTWNQGYIFLAI
                     DGRWDSNNDGQVGEGDQMLSYHPGRDSLYTPMVLQVHTDADQGGNVVLPLDLNIDTLM
                     AGIDIQALPVSHDVSDHTVRLMHQLSTAITHVQ"
     gene            1453..>3399
                     /locus_tag="PL106_03955"
     CDS             1453..>3399
                     /locus_tag="PL106_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA32255.1"
                     /translation="MFQALHSSAGAGKTHALVRHYLLLALREDEPGAYGRILALTFTN
                     KAASEMRERIITYLEGLAAGTELTGALANVRDTLVREAGLDPATVRERAARTLIHILH
                     HWPQLAVSTIDAFTRRVVMPFARDLRMDSELRMTTEEKELRARAVDRLLEDAGSDPAL
                     TTLLTSIGSDLVENEDVWRVDRPLLNLSDQLTKEAAIDPLLKLQDSQDQDFVAIQRDL
                     RRATHTFRERLRTLGRNALAALAAEGINADDLAYGKNGPWSYLNKLAVFDVALDPNDH
                     VRKAMAKDAWTSGKASASVKAAVERTAPLLRKTIQEVEDLRGSGELGAHTLRCAVLED
                     LLPAAGLHLLSDRLETVKREEALVFFSDLVKKVAAIVQREPAPFLYERLGEKYRHILV
                     DEFQDTSLLQWHALLPLITNALSTGGTALLVGDAKQAIYRWRNGEARQFTALPHLFGK
                     ERLADGDGHEGILLRTWAPIDPLLENRRSAPAVIAFNNDLFAALRDTLPEKYRIVYDA
                     PAQLAVNDQEGLVRVACFAKPEKNDAPGNDEHIDPDPLVFTAGAVREALDDGFKPGDI
                     AVLVRSGNQGNAVAAHLVVQGHQVVSPDALSLGADPGARTAMALLAWTCRPDDVSAAQ
                     AVQLLALLRPDHGTAPERGHPAEQM"
BASE COUNT          619 a         1134 c         1047 g          599 t
ORIGIN      
        1 ccggtgtgga tgctgaactg atagggcggc gtgccgatgc ccgtggacag ggtatcgaac
       61 cgcccctcga agatcaaaaa ccggtacatg gtggcccagg tccagtacat gcctgagcca
      121 gcacccaggt ccgatgacgg gtcgatcccc gtgacgtcca cgtgattcag gtcgtacggg
      181 agaccgaggc cgtaatgcag gctgtcgtac gtgcccaccg gcaggctgta gctccgtgct
      241 tccggtccgt tcaccagatc gagcagcacc gcatcggtgg cgcgagcggt ggtgtcgccc
      301 gagaacacca atgggctcag gtagaatttc acctgctgga tcagtacgcg ctcgttcgcc
      361 gcgctcaggt acacgttggt cttgtcgaag gctgttccgt cccaaatggg ccggaacgtg
      421 atccgcaata cgccgctggg cgtaccgccg ggtggcggca acggctcggg ttccaccggc
      481 gggtcgtgac ggcatccgac gagcatcgcc gaagcgagga ggagcactgc attcttcatc
      541 tattgcacgt gggtgatcgc ggtcgagagc tgatgcatca ggcggacggt atgatcgctg
      601 acatcatgcg ataccggcaa ggcctggata tcaatgcccg ccatcaaggt gtcgatgttc
      661 aggtccaacg gcagcaccac gttgcctccc tggtccgcgt cggtatgcac ttgcaacacc
      721 atcggcgtgt acagggaatc ccgacccggg tggtaactca gcatctgatc gccttcacct
      781 acttgtccgt cgttattact gtcccagcga ccatctatcg ccaggaagat gtagccctgg
      841 ttccatgtcc agtacatgcc attcacgccc aggggtgctt ccaccgttgc cggatcagcg
      901 tgattgttca cactgtccac tccgaggccg aagctcatgt cgtgcaaatg cccgctgagc
      961 tggccgatgt tgcggatcgt gcccccctcg gccaggtcca ccttcaaata cttgttcggg
     1021 aacgatgcca cactgtcgtg gttgtcgttg gtgaagtcga agtggcccat gtagaaacgg
     1081 agctgttcga gcttgtaccg caccccttga acgtcggtga ggaccatgct cgtatcgaag
     1141 gccgcaccat tgtgcgtgaa gctcatgctc atggccacca cggtgttggc accatcgggg
     1201 cccacgaggt tctcgtgcgt ctcctccaga tcgtccttct tgcaaccggc cagcaggacc
     1261 gcggtgctga acaaggccag gatcgtcttc ctcatgtgat cgggtttcgt tcctcgttga
     1321 tacgcgcgag cggatggtcc cgttgcacgc ttgaccaggt gcttccggcg aaagtagtcc
     1381 cgtgacccac gctccacgca atgagcccgg tcattttccc accagccttg tgctcccatc
     1441 ctactttgcg ccgtgttcca agcactccat agctcggccg gtgccggcaa gacccacgcc
     1501 ctggtgcggc actacctgct gctggcgttg cgggaagacg aacccggggc gtacggtcgc
     1561 atcctggcgc tcaccttcac caacaaggcc gccagcgaga tgcgcgaacg catcatcacc
     1621 tacctggaag gactcgcggc cggaacggaa ctgaccggtg ccctggccaa cgtgcgcgat
     1681 accctggtgc gcgaggcggg ccttgatccc gcgaccgtgc gcgagcgtgc cgcccgcaca
     1741 ctcatccaca tcctgcatca ctggccgcag ctcgccgtga gcaccatcga cgccttcacc
     1801 cgccgcgtgg tgatgccctt cgcccgcgac ctgcggatgg acagcgaact gcgcatgacc
     1861 accgaggaga aggagttgcg tgcccgggcc gtggaccgcc tgctggagga tgccggtagc
     1921 gaccccgcgc tcaccacatt gctcacctcc atcgggtcgg acctggtgga gaacgaggat
     1981 gtctggcgcg tcgatcggcc cctcctcaac ctctcggacc agcttaccaa ggaggccgcc
     2041 atcgatccct tgctgaaact gcaagactca caggaccagg acttcgtggc gatccagcgc
     2101 gacctgcgcc gtgccacgca caccttccgc gagcgcctgc gcaccctcgg ccgcaacgcc
     2161 ttggccgcat tggctgccga aggcatcaac gcggacgacc tggcctatgg caagaacggc
     2221 ccttggtcgt acttgaacaa gctcgccgta tttgacgtgg cgctggaccc gaacgatcac
     2281 gtgcggaaag ccatggcgaa ggacgcatgg accagcggca aggcatcggc atcagtgaag
     2341 gctgccgtgg agcggacagc cccgctgctg cgaaaaacga tacaggaagt ggaggatcta
     2401 cgggggtcgg gtgaactggg agcgcacacc ttgcgctgcg ccgtgctgga ggacctgctc
     2461 cccgcggccg gcctgcatct gctgagtgac cggctcgaaa cggtgaaacg ggaggaggcc
     2521 ctcgtcttct tcagcgacct ggtgaaaaag gtggccgcga tcgtgcagcg tgaaccggcg
     2581 cccttcctgt acgaacggct cggagaaaaa taccggcaca tcctggtcga cgagttccag
     2641 gacacctcgc tgctgcaatg gcacgccctg cttccgctga tcaccaatgc cctcagcacc
     2701 ggtggaacgg ccctgctggt gggcgatgcc aagcaggcca tctaccgctg gcgcaatggt
     2761 gaagcccgcc agttcaccgc cctgccccac ctcttcggca aggagcgcct ggccgacggc
     2821 gacggccacg aaggcatcct cctgcgcacc tgggcgccca tcgacccgct gctggagaac
     2881 cgccgcagcg cgccggccgt catcgccttc aacaatgacc tcttcgccgc gctccgcgat
     2941 accctgccgg aaaaataccg gatcgtgtac gacgcgcccg cccagctcgc cgtgaacgac
     3001 caggagggcc ttgtccgggt ggcgtgcttc gcgaagccgg agaagaacga tgctcccggg
     3061 aatgacgaac atatcgatcc tgaccctctg gtattcaccg ccggagccgt gcgcgaagcg
     3121 ctggacgatg gcttcaaacc gggcgacatc gccgtgctgg tgcgctcggg caaccagggc
     3181 aatgccgtgg ccgcgcacct cgtggtgcag ggccaccagg tggtgtcgcc cgatgccttg
     3241 tccctgggcg ccgatcccgg tgcgcgcacc gccatggcgc tgctggcctg gacctgcaga
     3301 cccgatgacg tgagcgctgc acaggccgtg caactgctcg ccctgctgcg acccgaccac
     3361 ggcacggcgc ccgagcgcgg gcaccccgcg gagcagatg
//
LOCUS       DAOQOC010000179         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13766_length_3388_cov_3.256826, whole genome shotgun sequence.
ACCESSION   DAOQOC010000179 DAOQOC010000000
VERSION     DAOQOC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13766_length_3388_cov_3.256826"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PL106_03960"
     CDS             complement(<1..1040)
                     /locus_tag="PL106_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNA32256.1"
                     /translation="MKLLRLIAAFAVALLSSISTFAQRGLPHGLASHEADLIRAYRDS
                     RASDDRGVTTPPPFAPRTMAEWEEVQTLCVSWISFPSILKQIVRYAKEECQVLILCSS
                     SGSNSQASISSYLLASNAGGPALANLNNISFLVTPYNSIWMRDYGAECIYQNEVDSLY
                     LLDWIYNRPRPNDDVSPDAIGTFKNKAVYSTTNAPNDLVHTGGNFMADGFGTAFSSNL
                     VIDENGPSGVYNQTDKTPAQVDAMMNTWMGINPGRYIRMATLPYDGIHHIDMHMKLLD
                     EETLLIGQFPAGTSDGPQIEANIAAIQQNYTSVYGTPYRIVRVPMPPSTGGAYAPSAD
                     YRTYTNSIFINRT"
     gene            complement(1123..2244)
                     /locus_tag="PL106_03965"
     CDS             complement(1123..2244)
                     /locus_tag="PL106_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32257.1"
                     /translation="MTRYLLFLLGCCLSFTSRAQCVFRNAFQSTLADCSVGMTWLELQ
                     TIGWPPTEQWTFSVDFGPIQPFLSHWTQIGGKWRYAVPLNAGVHTLDLPGYTDQLGTE
                     YCWDTYVFPTDLSVTPGDCGINLRIRAALDGALPSGALMTDGLRSAGLLPLAQPYTGL
                     GYTFVGSPTNMSITPSLLAVSGNDALVDWVVVEVRDAAAPSTILYSKPALLQRDGDVV
                     DADGDNYVNCPIAPAVYHVALRHRNHLGVMSASAHFHNVDPNISRHDFTGTLTGWYQG
                     AYGTNAQVLKGGIQRLWAGDATGNGQLRYTGAGNDRDPILTAIGGTTPNNMVSNVYDR
                     RDTNLDGVIKYTGTGNDRDVILINVGSTTPNSTRIQQLP"
     gene            2465..>3388
                     /locus_tag="PL106_03970"
     CDS             2465..>3388
                     /locus_tag="PL106_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNA32258.1"
                     /translation="MAKILVVDDEKAIRNALRDILEHEKHVVDEAEDGAAGLEKAKKG
                     SFDVVLCDIKMPKVDGLEFLQKLMAHNDEIPVIMISGHGNIDTAVDALKKGAFDFIEK
                     PPNISRILVAVRNALDRGNLVQETKTLRQKVQKEKTGVNRMVGESKALGEIRAMIEKV
                     APSDARVLITGGNGAGKEGVARMIHEKSGRASGPFIEVNCAAIPSELIESELFGHEKG
                     SFTSAIAQRKGKFELADGGTLFLDEIGDMSASAQAKVLRALQENRITRVGGDKDIKVN
                     VRVVAATNKELRKEIEAGTFREDLYHRLSVIP"
BASE COUNT          681 a          942 c         1137 g          628 t
ORIGIN      
        1 gtacggttga tgaagatgct gttcgtatag gtgcggtagt ctgcgctcgg tgcgtaggcg
       61 cctccggtgc tcggaggcat gggcacgcgt acgatgcggt agggcgtacc ataaacgctg
      121 gtgtagttct gctggatcgc ggcgatgttg gcttcgatct gtggaccgtc gctggtccca
      181 gcagggaact gtccgatgag cagggtctcc tcatcgagca gtttcatgtg catgtcgatg
      241 tgatggatgc catcgtaggg cagggtggcc atgcggatgt agcggccggg gttgatgccc
      301 atccaggtgt tcatcatcgc atccacctgt gcgggcgtct tgtccgtctg gttgtagaca
      361 ccgctcggac cgttctcatc gatcacgagg ttgctgctga aggccgtgcc gaaaccgtcc
      421 gccatgaaat tgccgccggt atgcaccagg tcgttcggcg cattggtggt gctgtacacc
      481 gccttgttct tgaaggtgcc gatggcatcc gggctcacgt catcgttagg gcgtggacgg
      541 ttgtagatcc aatcgagcag atagagactg tccacctcgt tctggtagat gcactccgca
      601 ccatagtcac gcatccagat gctgttgtag ggggtgacca ggaaggagat gttattgagg
      661 ttcgccagcg ccggtccacc ggcgttgctg gccagcagat aactgctgat gctcgcctgt
      721 gagttgctgc ctgaacttga gcagaggatg agcacctggc actcttcctt ggcatagcgc
      781 acgatctgct tgaggatgct ggggaaactg atccagctca cacacaaggt ctgtacctcc
      841 tcccactcgg ccatggtgcg cggcgcgaac ggcggcggcg tggtgacgcc ccggtcatcc
      901 gaggcccgag aatcgcggta ggcacgaatg agatccgcct cgtgcgaagc caacccatga
      961 ggtaacccgc gctgggcgaa ggtggagatg gacgaaagaa gagcgaccgc gaaggccgca
     1021 atgaggcgga gcagcttcat gcaggttggt gccgcgaaac taggccttgg cgaacgccgt
     1081 gcccgcggat ccgaccaagc cggagcgcga cgaggtcgcc ggctacggta actgttgaat
     1141 gcgtgtactg ttcggcgtgg tgcttcctac gttgatcagg atcacatcgc ggtcgttgcc
     1201 ggtgccggtg tatttgatca cgccatccag gttggtatcg cggcggtcgt acacattgct
     1261 caccatgttg ttcggtgtgg tacccccgat ggcggtgagg atggggtcgc ggtcgttgcc
     1321 ggcgcctgtg taccggagtt ggccattgcc cgttgcatcg cccgcccaga ggcgttggat
     1381 cccgcctttc aggacctgcg cgttcgtacc ataggctccc tggtaccacc cggtcagcgt
     1441 gcctgtaaag tcgtgccggc tgatgttggg gtccacgttg tggaagtgcg ccgaggccga
     1501 catcacgccg aggtggttcc gatgccgcaa ggcgacgtga tagaccgctg gggcgatggg
     1561 gcaattcaca tagttgtccc catcggcatc gaccacatct ccatcgcgtt gcagcagggc
     1621 aggcttggag tagaggatgg tgctgggtgc cgcggcatcc ctgacctcca ccaccaccca
     1681 gtccacgagc gcatcgttcc ccgaaacggc cagcagggat ggcgtgatgc tcatgttcgt
     1741 cggtgagccg acgaaagtgt atccgagccc cgtatagggt tgtgccagcg gaaggaggcc
     1801 ggccgatcgc agcccgtcgg tcatgagcgc accggagggt agtgccccat ccagtgcggc
     1861 acggatcctc aggttgatgc cgcagtcccc gggcgttacg gacagatcag tggggaacac
     1921 gtaggtgtcc cagcagtact cggtcccaag ctggtccgtg tatcccggga gatccagcgt
     1981 gtgtacgccc gcgttcaagg gcaccgcata gcgccatttc ccgccgatct gcgtccagtg
     2041 ggacagaaaa ggctgaatgg ggccaaaatc aacggaaaag gtccattgct cggtcggcgg
     2101 ccagcctatg gtctgaagct ccagccaggt catgccgacg gaacagtccg caagggtcga
     2161 ctgaaacgca ttgcggaaga cgcattgggc ccgtgatgtg aaggacaggc aacaaccgag
     2221 caggaagagc aggtaacgag tcatcgtggt gcctttgctg ggtaaagata tggttcctgg
     2281 tcccgtgaac ctgatccttg agcggcggtc agggcagaag atgcgtccga ggccgccgat
     2341 cctatccaag acgccagaag cccggccggt tgccggaaag ttaccacgtc gccgatgatc
     2401 cccgccgtcg gaacagaggg ttcggcattc agtcaccaac ttcaccgcct cctaaccgaa
     2461 aacgatggcg aagatcctgg tggttgatga tgagaaagcg atccgcaatg ccctgcggga
     2521 cattctggaa cacgagaagc acgtggtgga cgaggccgag gacggtgcgg ccgggctgga
     2581 gaaggccaag aagggcagtt tcgacgtggt gctctgcgac atcaagatgc ccaaggtgga
     2641 tgggctggag ttcctgcaga agctgatggc gcacaacgac gagatcccgg tgatcatgat
     2701 cagcgggcac ggcaacatcg atacggctgt ggacgcgttg aagaaaggcg ccttcgactt
     2761 catcgagaag ccgccgaaca tcagccggat tctggtggcc gtgcgcaatg cgctggaccg
     2821 gggcaatctc gttcaagaga cgaaaacgct caggcagaaa gtgcagaagg agaagaccgg
     2881 cgtgaaccgc atggtcggcg agagcaaggc gctgggcgag atccgcgcga tgatcgagaa
     2941 agtcgctccg agcgatgcgc gcgtgctgat cacgggaggc aacggcgcag gcaaggaagg
     3001 cgtagcgcgc atgatccacg agaagagcgg ccgtgcctca ggtcccttca tcgaagtgaa
     3061 ttgcgcggcg atccccagcg agctgatcga gagcgagctg tttggccacg aaaaggggtc
     3121 cttcacctca gcgatcgcgc aacgcaaggg caagttcgag ctggcggacg gcggcacgct
     3181 cttcctcgat gagatcggcg acatgagcgc atcggcccaa gcgaaagtgt tgcgggcctt
     3241 gcaggagaac cgcatcacgc gtgtaggcgg cgacaaggac atcaaggtga acgtgcgcgt
     3301 ggtggccgct acgaacaagg aactgcgcaa ggagatcgag gcgggcacgt tccgcgagga
     3361 cctctaccac cgcctcagcg tgatcccg
//
LOCUS       DAOQOC010000180         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13768_length_3388_cov_2.995800, whole genome shotgun sequence.
ACCESSION   DAOQOC010000180 DAOQOC010000000
VERSION     DAOQOC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13768_length_3388_cov_2.995800"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2162
                     /locus_tag="PL106_03975"
     CDS             <1..2162
                     /locus_tag="PL106_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32259.1"
                     /translation="WAGGLNFPQVSNIDLDGDGDKDLFFFDRTGDRVITLLSNQAQGS
                     STWSFSRAFDAVPPFRNLREWALLRDFNGDGKEDLFTYVTGGFACWKNTSTGNDLSFA
                     MYDTLIRSNYYPTEANLYVSQVDIPGIEDIDGDGDLDVITFSIFGTYIEYHRNMSMEY
                     YGTADSLEFEIYNRCWGFFSEDVNNNAVFLNDVCDYNVPDPRAAEEIIRRTAEERRAE
                     ARDPETQDRAHTGSTNLPIDLDGDGDKDLLVGDIISPTLLGLTNGGDQDSAHMVAQDI
                     LFPSYDTPVGLDVFPASFYEDVDHDGTRDLLVTANSETLSEDTAGVWYYHNTGTDASP
                     LFSFQQQDLFQRDMLDLGEGAYPVPFDFDGDGLMDLIVGNYGYFHSSGVYPSMLAALR
                     NTGTATAPAFTLVTRDYMGISTTGMGQGAYPAFGDLDGDGDADMLVGEFSGKLHYFQN
                     DPANGVAQFTLADPSVNSVVNGSTAELDAGILSTPQLFDVDGDGTLDLLVGERNGNVN
                     YYHMLQPAPDQLWELVNDTVGGVLAAEPPFNTGYSTPVMYLNEAGQRELLVGTQSGWI
                     KQYANIDGNLAGAWDLVEAHWQDIDDGEKSGVALHDWDGDGHMDVVVGNYRGGLSYWR
                     NDVITTVGGLDAPVGEDVFTLLPNPTEGPVTVELSMPLHAGMQVTVLDEQGRELWHVP
                     VRARRTPLSTDTLAPGVYMVRLSLGAQRWVQRLAVVR"
     gene            complement(2323..3264)
                     /gene="mdh"
                     /locus_tag="PL106_03980"
     CDS             complement(2323..3264)
                     /gene="mdh"
                     /locus_tag="PL106_03980"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012821192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNA32260.1"
                     /translation="MKITVVGAGNVGATCADACARWELANEVVLLDIKEGVAEGKALD
                     IWQTAPISLFDSRLTGSTNDYTRTAGSDVVVITSGLPRKPGMSRDDLIATNANIVKSV
                     TENVVKHSPDAIIIVVSNPLDVMSYCAYITSKLPSSRVIGMAGILDTARYRAFLATEL
                     NVSPKDIQAVLMGGHGDTMVPLPRYTTVGGIPVTELIAADKLNAIVERTKKGGGEIVN
                     LLGTSAWYAPGTAAAQMVEAIVRDQKRVFPVCAWLTGEYGLKNVYMGVPVILGRKGIE
                     RILELKLTKEEQELCNASAKAVKEVMDVLDKMNSVTA"
BASE COUNT          631 a         1078 c          993 g          686 t
ORIGIN      
        1 gctgggccgg tggcctcaac ttcccgcagg tctcgaacat cgacctggac ggggacgggg
       61 acaaggacct cttcttcttc gatcgcaccg gcgaccgggt gatcactctg ctgagcaacc
      121 aggcccaagg ctcgtccacc tggtccttct cgcgcgcctt cgatgcggtg cctcccttcc
      181 gcaacctgcg tgaatgggcc ctcctgcgcg acttcaacgg cgacgggaag gaggacctct
      241 tcacctacgt caccggtggt ttcgcctgct ggaagaacac ctccaccggc aacgacctgt
      301 ccttcgctat gtacgacacc ctgatccgct ccaattatta tcccaccgag gccaacctgt
      361 acgtgtccca agtggatatc cccggtatcg aggatatcga cggcgacgga gacctggacg
      421 ttatcacctt cagcattttc ggcacctaca tcgagtacca ccggaatatg agcatggagt
      481 actacggcac tgcggacagt ctggagttcg agatctacaa ccggtgctgg ggtttcttca
      541 gcgaggacgt gaacaacaat gcggtcttcc tgaatgatgt ctgcgactac aatgtgcccg
      601 acccgcgggc cgctgaggag atcatccgca ggaccgccga ggaacgcagg gccgaagcac
      661 gggacccgga aacccaggat cgcgcccaca ctggcagcac caacctcccg atcgatctgg
      721 acggggacgg cgacaaggac ctgctcgtcg gcgacatcat ctcccccacc ctcctgggcc
      781 tgaccaacgg tggcgatcag gacagcgcgc acatggtggc ccaggatatc ctcttcccgt
      841 cctacgacac gcctgtgggc ctggatgtct tccccgcttc cttctatgag gacgtggatc
      901 acgatggcac ccgtgatctg ctggtcacgg cgaacagcga gacgctctcg gaggacacgg
      961 ccggcgtatg gtactaccac aataccggta ccgatgccag tccgcttttc agtttccagc
     1021 agcaggacct cttccagcgc gacatgctcg atctgggcga aggggcctac cccgtaccct
     1081 tcgacttcga cggcgatggc ctgatggacc tcatcgtggg caactacggc tacttccact
     1141 ccagcggtgt gtatcccagc atgctcgccg ccttgcgcaa taccggcacg gccaccgcgc
     1201 cggccttcac gctcgttacc cgggattaca tgggtatcag taccaccggc atgggccagg
     1261 gcgcctatcc cgctttcggg gatctggatg gtgatggcga cgccgatatg ctcgtgggcg
     1321 aattcagcgg gaaactgcac tacttccaga acgatccggc caatggcgta gcgcaattca
     1381 ccctggccga tccctcggtg aactcggtgg tgaacggcag caccgccgaa ctggatgccg
     1441 gcatcctctc caccccgcaa cttttcgatg tggatggcga tgggaccctg gacctgctgg
     1501 tgggcgaacg caacggcaac gtgaactatt accacatgct ccaacccgca ccggaccagc
     1561 tctgggaatt ggtgaacgat acggtgggcg gggtactcgc ggccgagccg cctttcaaca
     1621 ccggttacag tactccggtc atgtacctga atgaagcggg gcagcgcgag ttgcttgtgg
     1681 gcacccagag cgggtggatc aagcagtacg ccaacatcga tggcaacctg gccggcgcct
     1741 gggacctcgt ggaggcgcac tggcaggata tcgacgatgg agaaaaatcg ggggtggccc
     1801 tgcacgactg ggacggagat ggccacatgg acgtggtggt gggcaactac cggggtggcc
     1861 tgagttactg gcgcaacgat gtgatcacta ccgtgggcgg gttggatgcg ccggtcgggg
     1921 aagacgtgtt caccttgctg ccgaacccga ccgaggggcc agttaccgtt gagctttcca
     1981 tgcccctgca cgctggcatg caagtgactg ttctggacga gcaaggtcga gagctttggc
     2041 acgtaccggt acgcgctcgc cgcacccccc tgtcgaccga tacgctggca ccgggtgtct
     2101 acatggtccg cctcagcttg ggtgctcagc ggtgggtcca gcgcctggcg gtcgtgcggt
     2161 gaggccgggc cccgcgaagc acaagcaccc tgcgttcctc ccggaccttg ggctccattt
     2221 ggccatggac agctatgggg cagacccggc cgagaatatc gtcatggtag gaactggaac
     2281 gcccaccggg ttggcgggcg ttccaactcg gtttgaaccg cctcaggccg tcacgctgtt
     2341 catcttgtcc agcacgtcca ttacctcttt cactgcctta gcactggcgt tacagagctc
     2401 ctgctcctct ttggtgagct tcaattccag gatccgctcg atgcccttgc ggcccaggat
     2461 gacggggacg cccatgtaga cgttcttcag gccatactca ccggtgagcc aggcgcaaac
     2521 ggggaatacc cgcttctggt cgcgaacgat cgcctccacc atttgcgctg cggccgtgcc
     2581 gggcgcatac caagcactgg tacccagcag attaacgatc tctccacccc ctttcttggt
     2641 acgctcaaca atggcgttca gcttgtccgc tgcgatcagt tcggttaccg gaatgccacc
     2701 gacggtagtg taacgcggca ggggtaccat ggtgtcgccg tggccaccca tgagcacggc
     2761 ctggatatcc ttggggctca cattcaactc ggtggccagg aaggcgcgat agcgggccgt
     2821 atccaggata ccggccatgc cgatcacacg actgctgggc aacttgctgg tgatataggc
     2881 gcaatagctc atcacgtcca gtgggttgct caccacgatg atgatggcat cagggctgtg
     2941 cttcaccacg ttctccgtca cgctcttcac gatgttggcg ttcgtggcga tcaggtcgtc
     3001 gcggctcatt cccggcttgc gaggcaaccc actggtgatg accaccacgt cgcttcccgc
     3061 cgtcctggtg taatcgttcg tactgcccgt caagcggctg tcgaacaggc tgataggtgc
     3121 cgtttgccag atgtccagcg cctttccttc ggccacgccc tccttgatgt cgagcagtac
     3181 cacttcattg gccagttccc aacgggcaca ggcatcggcg caggttgcgc ccacattgcc
     3241 cgctccaact acggtgatct tcatcggtca ggtctttttt tgttcggggg cgaaattaga
     3301 cgcgcaacca tggcgagggt attccgtctt atccacgatc ggtgacttct tttcaaattt
     3361 cccgctcttg tccagtcctc aagtcatc
//
LOCUS       DAOQOC010000181         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13838_length_3377_cov_2.999097, whole genome shotgun sequence.
ACCESSION   DAOQOC010000181 DAOQOC010000000
VERSION     DAOQOC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13838_length_3377_cov_2.999097"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PL106_03985"
     CDS             <1..565
                     /locus_tag="PL106_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA32261.1"
                     /translation="TANTGHELNGSTVGIIGFGRMGSAFAEKLRGFGVRTLAHDKYLS
                     NYAPEHVEETTLDDLLAHSDVISLHLPLTAETEHFADAGFFARCSKPIWFINTARGPL
                     VHTEALLDALDNGRVRGACLDTLEFEQRSLLGLRPDAPQHVLRRLFKHERVLLSPHVA
                     GITHESYFKLANVLADKILNDVTDGKL"
     gene            552..1460
                     /locus_tag="PL106_03990"
     CDS             552..1460
                     /locus_tag="PL106_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNA32262.1"
                     /translation="MGSSKLIYLAAVAALASGGCTYEPVTRPEIHGHRGCRGLLPENT
                     LPAFLEAAEMGCDLLELDVVIPADSQVLVSHEPWMSHRICRTPQGDSITEQQEHDFNI
                     YRMTVAEAQSFDCGSTPQVAFPDQELSPQFKPTLRQVVEAVDLKMMDNGTAGVGWNIE
                     IKSEPALYDVFQPRPERFARLVLAEIDSLGIGGRTVIQSFDPAILEEIHRTDETMATA
                     LLVDNTNGLERNLARLSYTPTHYSTAFALADKALVKTLDSLGIELLVWTVNDKSDLRR
                     MIKLGVGGIITDHPEKAIKLLDEEEE"
     gene            complement(1523..2614)
                     /locus_tag="PL106_03995"
     CDS             complement(1523..2614)
                     /locus_tag="PL106_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNA32263.1"
                     /translation="MRAPFRSLVRACLLPACLVLYVSTVHAQGARAREHKARTDSLAT
                     VVRGIEQRITTADSLRDPVTAAQARLELAALVHPKTALQLVEEAVAALDTAGAPELAL
                     RAHQELADRYTASKAYEKANAQWSIIVAITGRMRDEAKGNAEARATEIARLEGEVEDL
                     TGRLDRAVGVMAQRQDAQARREALSLDAMVAGLVLLALSVVFFTWHAGRQRKALERLR
                     IEKDHLRAEAAEALRAKELRPPSVPVAPPGTATTAAPAPPVAPARVVPCATFTGPTAD
                     DAMLLAMVRRMGEARLNTLRDARARGDNDKVVRVVHTMKPQLVAIDAPYFQDLCGRIT
                     GAVPGADPVRWAEDLDRFEQGMARVLGRA"
     gene            complement(2710..>3377)
                     /locus_tag="PL106_04000"
     CDS             complement(2710..>3377)
                     /locus_tag="PL106_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNA32264.1"
                     /translation="LGREGSDFSAAIFAYALGAESVTIWKDVPGMFNADPNKFPDTRL
                     LEHISYKEAIELSYFGASVIHPRTLQPLQRKGIPLYVRSFADIGAKGSTIDHRSDNDS
                     LVPSFILKPGQLLISITPRDLSFVVEENLSSIFKRFAEHSVRIELMQNSAVAFTVCVD
                     DTPRARQLIGELHREYEVRYNEGCELITVRHFDEATLIGLLAGREPLLEQRSRVTARF
                     VVK"
BASE COUNT          635 a         1119 c         1043 g          580 t
ORIGIN      
        1 cacggcgaac accggtcacg aactcaacgg cagcaccgtg ggcatcatcg gcttcggacg
       61 catgggcagc gccttcgccg agaagctccg tggattcggt gtgcggaccc tcgctcacga
      121 caaatacctg agcaactacg cgcccgagca cgtggaggag accaccctgg acgacctgct
      181 cgcgcacagc gacgtgatca gcctgcacct gccgctcacc gcggagaccg aacattttgc
      241 ggatgccggc ttcttcgctc gttgcagcaa gcccatctgg ttcatcaaca ccgcgcgcgg
      301 cccgctggtg cataccgagg cactgctcga tgcgctggac aacggccgtg tgcgcggcgc
      361 atgcctggac acgctcgaat tcgagcaacg ttcgctgctc gggttgcgcc cggacgcgcc
      421 gcaacacgtg ctccgcaggc tgttcaagca tgaacgcgtg ctgctctcac cccatgtggc
      481 gggaatcacc catgagagtt acttcaagct ggccaacgtg ctggccgaca agatcctgaa
      541 cgatgtgacg gatgggaagc tctaagctca tttaccttgc cgcagtggcc gctctcgctt
      601 cgggcggctg cacctacgag cccgtgaccc gccccgagat acatggccac cgcggttgcc
      661 gcggcctgct gcccgagaac accctgccgg ccttcctgga agcagcggag atgggttgtg
      721 accttctgga gctcgatgtg gtgatccccg ccgacagcca ggtgctggtg agccacgagc
      781 cgtggatgag ccaccgcatc tgccgcacgc cgcaaggcga cagcatcacc gagcagcagg
      841 agcacgactt caatatctac cgcatgaccg tggccgaagc gcaatccttc gactgcggca
      901 gcacgcctca ggtcgccttc cccgaccagg agctctcgcc gcagttcaaa cccaccctgc
      961 gccaggtggt ggaggcggtg gacctgaaga tgatggacaa cggcaccgcc ggtgtcgggt
     1021 ggaacatcga gatcaagagc gagcccgcgt tgtacgacgt tttccaaccg cgaccggaac
     1081 gtttcgcccg gctggtgctc gccgagatcg acagcctggg catcggtggc cgcaccgtga
     1141 tccaatcctt cgatcctgcc atcctggaag aaatacaccg caccgacgag accatggcca
     1201 cggcgttgct ggtggacaac accaacggcc tggaacggaa cctggcccgc ctcagctaca
     1261 cgcccaccca ctacagcaca gccttcgcac tggccgacaa agcgctggtg aaaacgctcg
     1321 actccctggg gatcgaactg ctggtgtgga ccgtgaacga caagagcgac ctgcgccgga
     1381 tgatcaagct cggggtgggc ggcatcatca ccgaccatcc ggaaaaagcc atcaagctgc
     1441 tggacgaaga ggaggaatag ggcgcgtccg cacggtcact tttcgcagac atccacacgt
     1501 tcgggcagga tcgcgggcgt gatcaagccc gacccagcac acgcgccatg ccctgctcga
     1561 accggtcaag gtcctcggcc cagcgcacgg gatcggcacc gggtacggca cccgtgatgc
     1621 gaccacaaag atcctggaaa taaggggcat cgatggcgac gagttgcggc ttcatggtat
     1681 gcacaacgcg cacgaccttg tcgttgtcgc cgcgcgcacg ggcatcacgc aaggtgttca
     1741 ggcgcgcttc gcccatgcgg cggaccatgg ccagcagcat cgcatcgtcc gcggtcggcc
     1801 ccgtaaacgt ggcgcacgga acgacccgtg cgggagcgac cggtggtgca ggcgccgcag
     1861 ttgtcgctgt tcccggtgga gcgaccggaa ctgatggggg gcgaagctcc ttcgcccgca
     1921 acgcttctgc cgcctcagca cgcaaatgat ccttctcgat ccgtaaccgc tccaaggcct
     1981 tccgctgccg gcccgcatgc caggtgaaga atacgaccga cagcgccagc agcaccagtc
     2041 cggccaccat ggcatcaagc gacagggcct cccggcgcgc ctgtgcgtcc tgccgttgtg
     2101 ccatcactcc gaccgcacga tcgagcctgc cggtgagatc ctccacctcc ccttccagac
     2161 gcgcgatctc cgtggccctg gcttctgcgt tacccttcgc ctcgtcgcgc atgcgtccgg
     2221 tgatcgcaac gatgatcgac cactgcgcgt tggctttctc gtatgctttc gatgcggtgt
     2281 accggtccgc cagctcctgg tgggcgcgca aggcgagctc cggcgcaccg gcggtgtcca
     2341 gtgcggcaac ggcctcctct accagttgga gcgcggtttt cggatggacc agggcggcca
     2401 gctccaagcg ggcctgtgcg gcagttaccg ggtcgcgcag ggaatcggcc gtagtgatgc
     2461 gctgttcgat cccacgcaca accgttgcca acgagtcggt ccgggccttg tgttccctgg
     2521 cacgtgcgcc ttgggcatgc actgtggaaa cgtacaggac aagacatgcg ggcaggaggc
     2581 acgcacgaac aagggagcgg aacggggcac gcattcggtg tgcgaaacta cacggtcctc
     2641 caggcgccga tctccgccgc ctgggccgaa gtttcctccg ggttctccac agccggaaag
     2701 tcacgaggat cacttcacca caaaacgggc cgtgacgcgt gatcgttgct caagcaaggg
     2761 ctcgcgaccc gccaggagcc cgatcaatgt ggcctcatcg aagtggcgca cggtgatcag
     2821 ctcgcacccc tcgttgtacc tcacttcgta ttcgcggtga agttccccta tcagttgccg
     2881 tgcgcggggc gtgtcatcca cgcacacggt gaaggccacg gcactgttct gcatcaactc
     2941 gatacgcacg ctgtgttcgg cgaagcgctt gaagatgctg ctgaggttct cctccaccac
     3001 gaagctgagg tcgcgcgggg tgatgctgat gagcagttgc cccggtttga ggatgaagga
     3061 cggcaccagc gagtcgttgt cgctgcgatg atcgatcgtg ctgcccttgg cgccgatgtc
     3121 ggcaaaggag cgtacgtaca gcgggatccc cttgcgctgc agcggttgca gggtgcgcgg
     3181 gtggatcacg ctggcaccga agtagctcag ctcgatcgct tccttgtaac tgatgtgctc
     3241 gagcaatctc gtgtcgggga acttgttcgg atcggcattg aacatgccgg gcacatcctt
     3301 ccaaatggtg acgctctccg cgcccagcgc ataggcgaag atcgccgcgc taaaatccga
     3361 tccctcgcgc cccagcg
//
LOCUS       DAOQOC010000182         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13875_length_3372_cov_3.189629, whole genome shotgun sequence.
ACCESSION   DAOQOC010000182 DAOQOC010000000
VERSION     DAOQOC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13875_length_3372_cov_3.189629"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PL106_04005"
     CDS             complement(<1..537)
                     /locus_tag="PL106_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32265.1"
                     /translation="MKGNSETCGSCALAIARSLIAACMILLAPFVRAQRSDIDSLRTI
                     LGHAADDTSYAKACYYLARSFYFEGDLDSARAYGDRGSAQCLRVGHPDPRKEFWLVQL
                     CRVRGMSWYSASRFDSAIVAFQQMFRYAEKLHIAKDMGAALSYQGFALREIDDQQGAL
                     AMVRRAITVLDTLPPGPDI"
     gene            complement(553..2400)
                     /locus_tag="PL106_04010"
     CDS             complement(553..2400)
                     /locus_tag="PL106_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA32266.1"
                     /translation="MRPLNHLLIFLRVLVAVPLYGQDHLGDSLATVARTAKDDSVRIH
                     ALRGLAGNWLYAHGQMDSTIAVVGRAVKVYDRLPPERQNTLTRDRLKLYEYAGFALHQ
                     TGHLDEALLRFQAGARMAEEKDLPVEVAGFYMYMSQVFGAMHDNTSVRTYSQRALDIL
                     EPMGPSDDLALARYTMAAYHVHEEHWDSAEHYAKLALTFHVQEHDASRQMAEETMLLQ
                     IYHRTRQSDSAEAHLGTAERIAAQVDYPPSLVILQGHRARIELFRGQYTEAIASIDSS
                     IALSSRLGLPFDRSEAYRVRSIALAALHRVNEAFMASDSVQAIMLRDMGLQEQRKLTE
                     VRMGFERDKERALATADLARQRTRTGLATGLALLGAVIAVVLWHLYRLRARTAVALRE
                     KNMEIRRAQDQLVESEKQHEAEKVRTRIARDIHDEIGATLTKIRLLSDVAASIPFEQH
                     AEMQRSLQRIGEHAKLVSTSMSDIVWAVDPARDTHQGMLDHVRELAHRLLGDNGITYD
                     LDLTCTLGSAVLEPELRRDIHLLINEALNNILKYARAGNVKLSILLNTHHFTLRIQDD
                     GAGFDPSRTAGGGNGLRNMRDRAQRHGGSFRISSTPGKGTCVDAHGAIH"
     gene            complement(2402..>3372)
                     /locus_tag="PL106_04015"
     CDS             complement(2402..>3372)
                     /locus_tag="PL106_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNA32267.1"
                     /translation="SLSTINEGVDLPGGEPFARMIDAITPMPPKEHFIAVLSRAMGVT
                     QDMAKLLSARLFETGLIEYFDDERKEHSRFDRQLHLFDVLSPVKDHQENIERQDRLNR
                     AQVLVLGLGGVGHQVALSLAASGVGSLVLVDDDVVEESNLHRQVLFTHADVRRPKVDA
                     AREGLLRIAPACHVQVCRAMVGSQEQWRSIIATFPGTRYVMISADRPVQLVSWISTAR
                     AEFHYHAIKCGYMGVQGIIGPMVGPDTSGYDELFESWGPLVDAQTSAVKRINEMAVAP
                     TMAASNAIMANIAALELIKHITGVGGLRLMGCRLLLDLRDYSTCFG"
BASE COUNT          624 a         1043 c         1047 g          658 t
ORIGIN      
        1 gatgtcaggt ccggggggaa gtgtatcgag tacggttatc gcgcgccgta ccatggccag
       61 tgcgccctgc tgatcgtcga tctcccgcaa cgcgaaaccc tggtaggaca aggcagcgcc
      121 catatccttg gctatgtgca gcttctccgc gtaacggaac atctgctgga aggccacgat
      181 ggccgaatcg aaacgggaag ccgagtacca actcatgccg cggacgcggc agagctgcac
      241 aagccagaac tccttgcgcg gatcggggtg accgacccgc aagcactgag ccgagccgcg
      301 atccccgtac gcccgcgcac tgtccagatc cccctcgaaa tagaagctcc tggccagata
      361 gtagcatgcc ttcgcgtagc tggtgtcatc cgccgcgtgg cccaagatgg tacgcaggct
      421 gtcgatgtcc gaccgttgcg ccctgacgaa cggggccaga aggatcatgc acgccgcgat
      481 gagtgaacgc gcgatggcga gggcgcacga accgcatgtc tcgctgttgc ctttcattcc
      541 ttttcgacgt gatcaatgta tcgcaccatg tgcatccacg catgtgccct tcccgggggt
      601 ggagctgatc cggaagctgc cgccatggcg ttgcgcgcga tcgcgcatgt tgcgcaggcc
      661 attgccccca ccggctgtcc gggaaggatc gaatccggca ccgtcgtcct gtattctcaa
      721 ggtaaaatga tgtgtgttta gcagtatgga cagcttcacg ttccctgctc gggcgtactt
      781 gaggatgttg ttcagcgctt cattgatgag caggtggatg tcgcgacgca gttcgggctc
      841 cagtaccgcc gagcccaaag tgcaggtcag gtcgaggtcg tacgtgatgc cgttgtcccc
      901 cagcaggcgg tgcgcgagtt cgcgcacatg gtccagcatg ccctggtgcg tgtcgcgcgc
      961 gggatccacc gcccaaacga tgtcgctcat ggatgtgctg accaacttgg cgtgctcccc
     1021 gatccgttga agtgaccgtt gcatttcggc gtgctgttcg aacgggatgg acgcggccac
     1081 atcgctcagc aggcggatct tggtgagcgt ggcaccgatc tcgtcgtgaa tgtcgcgagc
     1141 gatgcgtgtg cgcaccttct ccgcctcgtg ctgcttctcg ctttccacaa gctgatcctg
     1201 cgcgcggcgg atctccatgt tcttctctcg cagggcgacg gccgttcgcg cccgaagccg
     1261 gtataggtgc cagagcacga cggcaatgac ggccccgagg agggccagtc cggtggcgag
     1321 cccggtgcgc gtgcgttgcc gtgcgaggtc cgccgtggcc aacgcccgtt ccttgtcccg
     1381 ttcgaagccc atgcgcacct ccgtgagctt gcgctgctcc tgcaggccca tgtcgcgcaa
     1441 catgatcgct tgcacactgt cgctggccat gaaggcctcg ttcacccggt gaagggcggc
     1501 gagggcgatg gaacgcacac ggtaggcctc ggaacgatcg aaaggaaggc ccagtcgcga
     1561 gctcaaggcg atggacgaat cgatgctggc gatcgcttcg gtgtattgac cacggaacag
     1621 ctcgatccgt gcgcggtggc cttggaggat cacaagtgag ggtggatagt ccacctgggc
     1681 tgcgatgcgt tcggcggtgc ccaaatgtgc ttcggccgag tcggattggc gtgtgcgatg
     1741 gtagatctgc aacagcatgg tctcttcggc catctgtctg gaggcgtcgt gctcttgcac
     1801 atggaaggtc agggccaact tggcgtaatg ttccgcgctg tcccaatgct cctcatgcac
     1861 atggtacgcg gccatggtgt aacgcgcgag agcgagatca tcgctcgggc ccatgggctc
     1921 caggatgtcc agtgctcgct gcgagtaggt gcgcacgctg gtgttgtcgt gcatggcgcc
     1981 gaacacctgc gacatgtaca tgtagaagcc cgcgacctcc acaggtagat ccttctcttc
     2041 ggccatgcgt gctccggcct ggaagcggag cagcgcctcg tccaggtggc cggtctgatg
     2101 caaggcgaaa cccgcatatt catacagttt gagccggtcc ctcgtcaggg tattctgccg
     2161 ttccggcggc aaccgatcat acaccttcac tgctcgaccc accactgcga tcgtactgtc
     2221 catctgcccg tgcgcataaa gccagttgcc tgccagaccg cgcagggcat ggatgcgcac
     2281 ggagtcgtcc ttggccgtgc gtgccacagt ggcgaggctg tcgcccagat gatcctgccc
     2341 gtacaagggc accgccacga ggacgcggag aaagatcagc aggtggttga gcggacgcat
     2401 ctcagccgaa gcaggtggag taatcgcgaa gatcgagcag taaacggcat cccatcaagc
     2461 gtagaccgcc caccccggta atgtgcttga tcagctccag tgctgcaatg ttcgccatga
     2521 tggcattgct cgcggccatc gtgggggcga cagccatttc gttgatgcgc ttcaccgcag
     2581 aggtctgtgc atccaccagt ggcccccagg attcgaagag ttcatcgtag ccggatgtat
     2641 cgggcccgac catcggcccg atgatgccct gcacccccat gtagccgcat ttgatggcgt
     2701 ggtagtggaa ctcagcgcgt gctgtgctga tccaactgac gagttggacg ggcctgtccg
     2761 cgctgatcat cacgtaccgc gttccaggaa aggtcgcgat gatcgagcgc cactgctcct
     2821 ggctaccgac catggccctg cacacttgca catggcacgc aggggctatc cgcaacaatc
     2881 cttcgcgtgc ggcatccact ttgggcctgc gcacgtcggc atgggtgaaa agtacctgac
     2941 gatgcaggtt gctctcctcc accacatcat catccaccaa caccagggag ccgaccccgg
     3001 acgcggccag cgagagggcc acctggtggc cgaccccgcc caggcccagc accagcacct
     3061 gcgccctgtt gaggcgatcc tggcgctcga tgttctcctg atggtccttc accggagaaa
     3121 gcacatcgaa aaggtgcaat tgccggtcga aacgcgaatg ctccttccgc tcgtcatcga
     3181 aatactcgat gagaccggtc tcgaacaagc gtgccgaaag cagcttggcc atgtcctgcg
     3241 ttacgcccat tgcgcgcgag agaacagcga tgaagtgttc cttgggcggc atcggtgtga
     3301 tggcgtcgat catacgggcg aacggctcac ctccggggag gtccacccct tcattgatcg
     3361 tggacagcga ac
//
LOCUS       DAOQOC010000183         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13912_length_3366_cov_5.678043, whole genome shotgun sequence.
ACCESSION   DAOQOC010000183 DAOQOC010000000
VERSION     DAOQOC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13912_length_3366_cov_5.678043"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PL106_04020"
     CDS             complement(<1..157)
                     /locus_tag="PL106_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNA32268.1"
                     /translation="MQLRRVVITGLGALTPLGNTLKDYWEGLVSGRSGAAPITRFDAS
                     KFKTRFAC"
     gene            complement(170..406)
                     /locus_tag="PL106_04025"
     CDS             complement(170..406)
                     /locus_tag="PL106_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNA32269.1"
                     /translation="MSDIKSRVIAIIVDKLGVDQGEVTTEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIQTVGQAVDYVEKNAK"
     gene            complement(588..710)
                     /locus_tag="PL106_04030"
     CDS             complement(588..710)
                     /locus_tag="PL106_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32270.1"
                     /translation="MTPPPVDAAPVLVVSPTDLLVISLCGIAVVALGVMPGWVM"
     gene            complement(707..1147)
                     /locus_tag="PL106_04035"
     CDS             complement(707..1147)
                     /locus_tag="PL106_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA32271.1"
                     /translation="MATWLVDTNVWLALLEERHAFHRTTVAWWNALHMDDRVLFCRST
                     QQSFLRLRTTDALTMAYGLEPDTNATAWQAYQTLRKDERIELVDEPVTLEAEWKALAA
                     VGMASPKLWMDAYLAAFAIAGSFRLVTIDKAFKQFKRLNLLLLQ"
     gene            complement(1147..1398)
                     /locus_tag="PL106_04040"
     CDS             complement(1147..1398)
                     /locus_tag="PL106_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32272.1"
                     /translation="MKTTLELPDELMRAIKVRAAEEGITIKDLLTDLLRRALGPVKTT
                     KKRVLKYRELPVRRGGKPATPAEELTPDRVARILWGSSK"
     gene            1740..2039
                     /locus_tag="PL106_04045"
     CDS             1740..2039
                     /locus_tag="PL106_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA32273.1"
                     /translation="MSNNVKRAEHRAAMLAHVADHEQSGTSRRAYCAEHGLSEQTLAY
                     WIKRTRLDGAADKGGFTAVEVTTAETLELCYPNGVRLLLPTNTALRQVAACIKLY"
     gene            2052..2411
                     /gene="tnpB"
                     /locus_tag="PL106_04050"
     CDS             2052..2411
                     /gene="tnpB"
                     /locus_tag="PL106_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HNA32274.1"
                     /translation="MLSLTSAHHYLLRRVPTDMRKGVDTLCGVVRDELGRDPLSGEVF
                     VFIGSRRDRIKLLLYESGGYVLWYKRLEKGTLEIPVGDGAVRMNWRELVLMIEGVPLV
                     QPRTRLRWNAPQSTAPC"
     gene            2467..>3366
                     /locus_tag="PL106_04055"
     CDS             2467..>3366
                     /locus_tag="PL106_04055"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNA32275.1"
                     /translation="MVATEQDVKDAHIAQLEGQVAQLTFQLEQLKRMIFGAKSERFVP
                     TDPQQATLFGTSAGSVPTTGTPPTQAISYTRAKAQPKQQPVREAIAAHLPRIEEIIEP
                     KDLPEGARKIGEEVTETLEYTPGSIEVRRVVRPKYVHEDTSAGSVILIAPLPSLPFPK
                     SNLGGSLAAHICVAKFADHIPLYRQRAQLKRAGLDVSDSTIGGWFQATATLLEPLGDV
                     LKKEVLAQDYLQVDESPIPVQDDHNEKGIRKGYQWVYHAPLTKVVLYDYRPGRSAEFP
                     TEVLRDFHGTLQTDGYAGYEKLAA"
BASE COUNT          683 a         1055 c         1016 g          612 t
ORIGIN      
        1 cgcaggcgaa acgggtcttg aacttgctgg cgtcgaagcg ggtgatgggc gcggctccgc
       61 tacggccgct caccaggcct tcccagtaat ccttcagggt attaccgaga ggggtgagcg
      121 cgcccagacc ggtgatgacc acgcgtctga gctgcatgcc gcagtgcggt tacttcgcgt
      181 tcttctccac gtagtccacg gcctgaccca cggtctggat cttctcggcc tggtcatcgg
      241 ggatcgcgat gttgaattcc ttctcgaact ccatgatgag ctcgacggta tcgaggctgt
      301 cagcgccaag gtcgttcgtg aagctcgcct ccgtggtcac ttcgccctga tcgaccccga
      361 gcttgtccac gatgatcgcg atgacccttg acttgatatc cgacatgtcc gttcgttttt
      421 tgtgtgtggg ctgcaaagga aagggaaaat cacgtgacgc aagcacccct gccccactca
      481 aaggaaatag ctgagcatca gttgtttgcg caacaaatgg gcgtatcgac cctccacctg
      541 aaaggctccg gtggtacaag aaagcctgga tcctcttgcg gagcgcctca catcacccac
      601 cccggcatca ccccgagcgc caccacagcg atcccgcaca gggagatcac caggagatcg
      661 gtcggcgaca ccacgagcac gggtgcggca tcgacgggcg gcggcgtcat tgaagcagca
      721 agagattgag ccgcttgaac tgcttgaagg ccttgtcgat ggtcaccaga cggaaggatc
      781 ccgctatggc gaaagccgct aggtacgcgt ccatccacag tttcggtgat gccataccga
      841 ccgcagcgag tgctttccac tccgcctcca acgtcactgg ctcatctacc agttcaatgc
      901 gttcatcctt cctgagggtc tgataagcct gccaggccgt cgcgttcgta tccggttcca
      961 acccgtaagc catggtgagc gcatccgttg tccgcaagcg aaggaaactc tgctgcgttg
     1021 accggcagaa caggacgcga tcgtccatgt gaagcgcgtt ccaccatgcg accgtcgtgc
     1081 ggtgaaatgc gtgccgctct tccaacagcg ccagccagac gttggtatcc accagccagg
     1141 tcgccatcac ttgctgctgc cccaaagtat ccgcgccaca cgatcggggg tcaactcctc
     1201 ggctggtgtg gccggtttcc cgcctctgcg caccggaagc tccctgtatt tgagtacgcg
     1261 cttcttcgtc gtcttcacag gtcccaatgc cctccgcaac aggtcggtga gcaggtcctt
     1321 gatcgtgatg ccctcttccg cggcccggac cttgatcgcc cgcatcaatt catcgggcag
     1381 ttccaatgtc gtcttcatac tgcgaatata caggtatatg ggaacccata ttcctgggta
     1441 ctggtggcgg acccagcctg ttcggtgtgc cgttggaaag cctcgtgcat ggcccggccg
     1501 atcgatctct tcggcgacag gggccgaaat gctgcgaacg ggatggggat cgcgctcatc
     1561 accaatgtgg tgagcgggtc cgccagcgtc aagtctttgg ccgtggtact ggtagcagtt
     1621 cccccaacca accagaacca gccatggagt aagcctccgg cgaagtacat cttgatgggc
     1681 tgaagatagc tctggtgctt tgcggcctaa cgccttcgcc gggctacggc gagcaaagca
     1741 tgagcaacaa tgtaaagcgc gcggagcacc gcgcggcgat gctggcccac gtggccgacc
     1801 acgagcaaag tggaacgagc cgtagagcgt actgtgccga gcacgggttg agcgagcaga
     1861 cgctggcgta ctggatcaag cgcacacggc tggacggagc tgcggacaag ggcggtttca
     1921 cggcggtgga agtgaccacg gcggagacct tggaactgtg ctaccccaat ggcgtgcggc
     1981 tgctgttgcc gacgaacacg gcactgcgcc aagtggccgc gtgcatcaag ctgtactgat
     2041 caggccgaag gatgctcagc ctcaccagcg cgcaccacta cctgctgcga cgcgtgccca
     2101 ccgacatgcg caagggcgtg gacacgctct gcggggtggt gcgcgatgaa ctgggccgcg
     2161 acccgctcag cggggaagtg ttcgtcttca tcggatcgcg acgcgaccgc atcaagctac
     2221 tgctctatga gagtggcggc tacgtgctct ggtacaagcg cctggagaag ggcaccctgg
     2281 agatccccgt gggggatggt gccgtgcgta tgaactggcg ggagttggtg ctgatgatcg
     2341 aaggcgtacc gctggtgcag ccacgcacga ggctccgctg gaacgcgccg cagagcacgg
     2401 cgccttgttg acaaaggctc ataaccggat cttgacaaaa cgcgcgcgcg atcggaagtt
     2461 cgtgctgtgg tcgccacgga gcaggacgtc aaggacgcgc acatcgcgca gctcgaaggg
     2521 caggtggccc agctcacctt ccagctcgag caactcaagc gcatgatctt cggcgccaag
     2581 agcgaacgct tcgtgcccac cgatccgcag caggccacac tcttcggcac ttcggcaggc
     2641 tcagtgccga caacgggaac gccgcccacc caagcgatca gctacacacg cgccaaggcc
     2701 cagcccaagc agcagcccgt gcgcgaagcc attgccgcac acctgccacg catcgaagag
     2761 atcatcgaac ccaaggatct tcccgaaggg gccaggaaga tcggggaaga ggtcaccgag
     2821 accctggagt acacccccgg ctcgatcgag gtgcgcagag tggtgcgccc caagtatgtg
     2881 cacgaagaca cttcggcagg ctcggtgatc ctgatcgccc cattaccatc gctgcccttc
     2941 cccaagagca atctgggcgg atcgctcgcc gcacatatct gcgtggccaa gttcgccgac
     3001 cacatccccc tgtaccgtca acgcgcccag ctcaaacgcg ccgggctcga tgtgagcgac
     3061 agcaccatcg gcgggtggtt ccaggccacg gccaccttgc tggagccctt gggcgatgtg
     3121 ctcaagaagg aagtgctcgc acaggactac ctgcaggtgg acgagagtcc gataccggtg
     3181 caggatgatc acaacgagaa gggcatccga aaaggctacc aatgggtcta tcatgcaccg
     3241 ctcaccaaag ttgtgctcta cgactatcgt ccgggacgtt cggcggagtt ccccaccgag
     3301 gtgttgcgcg acttccatgg caccttgcag accgatggct acgcaggtta cgagaagctc
     3361 gcggca
//
LOCUS       DAOQOC010000184         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13938_length_3363_cov_4.446493, whole genome shotgun sequence.
ACCESSION   DAOQOC010000184 DAOQOC010000000
VERSION     DAOQOC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13938_length_3363_cov_4.446493"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PL106_04060"
     CDS             complement(<1..1519)
                     /locus_tag="PL106_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA32276.1"
                     /translation="MLSRLLSALTLVVLATSSAFAQVGSGTLQGTVKDKKTGEALPFV
                     SVVVENRGTRVAGGQTDFDGLFKIGPIEPGTYDVTVSYVGYQPMKQTGVIINSNKITF
                     LEPELTSGVDLKEVEVVRYKVPLIDKDGGASGSTVTREQIARMPARSAQGVATTVAGA
                     STAGTGGGISIRGARSENTYYYIDGVKVPAGAGTGLPKSAIEEVQVVTGGVPANYGDV
                     TGGLVNITTRGPGRTFFGGLEYLSSGWKVGEDVTDIVGLDKYAFNQVEASLSGPVFFK
                     KDSAGNKTRPLLGFFLSGQFTDAVDGGPLYGGDLRVKKDVRDDLVRNPMAVRVIGNDV
                     AAVYRSNYLKADDVERIKTRQNAASRTYLGSVKFDIATTPSINFTVGGSLDYNKRSDF
                     NRNNSLLNAENNQQVRDFTYRGFVRFTQRFNSREEEAGGEAKKKGGIRNAYYTLQLDY
                     SKRQQNVEDPTHKDKFFDYGYIGRYQRFSSPSFAQVEGRYEQTGFLDTLVTFSPGT"
     gene            complement(1973..2290)
                     /locus_tag="PL106_04065"
     CDS             complement(1973..2290)
                     /locus_tag="PL106_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32277.1"
                     /translation="MMRLFRAVFMFAVLLTLPLVAHAQDGDGGGNSAGISRKQQERNL
                     AKKERKDKKSLAKEEKRLLKQHRQHQDKATRKRMKRNKRSSDRNGPRGHRDPFLQRLF
                     RKH"
     gene            2395..2817
                     /locus_tag="PL106_04070"
     CDS             2395..2817
                     /locus_tag="PL106_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32278.1"
                     /translation="MNRFLLFSSLVIALLSVSCKKESTTGVPPVQLDVSININLPEYA
                     DLQVVGGWVYLTGGSQGLIVYRKNQDEFSALDRHCPFQPENLCHVIVDESGVIARDTA
                     CCHSAYLIMDGQPTEGPSSFPLKAYHTTFNGTMLRIYN"
     gene            complement(2908..>3363)
                     /locus_tag="PL106_04075"
     CDS             complement(2908..>3363)
                     /locus_tag="PL106_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNA32279.1"
                     /translation="VTEAAFRKMKDKTIVCNIGHFDNEIDMAWLNKAYGSTKVEIKPQ
                     VDKYTIDGKDIIILAEGRLVNLGCATGHPSFVMSNSFTNQTLAQLELWKNHKNYENKV
                     YVLPKHLDEMVASLHLAKIGVELEELTEAQAKYIGVPKNGPFKSDAYRY"
BASE COUNT          625 a          999 c         1007 g          732 t
ORIGIN      
        1 cggttcccgg actgaaggtc accagtgtat cgaggaagcc ggtctgctcg tagcgacctt
       61 cgacctgggc gaagctgggc gaggagaagc gctgataacg gccgatgtag ccataatcga
      121 agaacttgtc cttgtgcgtg ggatcctcca cgttctgctg ccgcttggaa tagtccaatt
      181 ggagggtgta ataggcattg cggatgccgc ccttcttctt cgcctcgcca ccggcctctt
      241 cttcgcggga attgaatcgc tgggtgaagc gcacgaaacc gcgataggtg aagtcgcgca
      301 cctgctggtt gttctcggcg ttgagcaggg agttgttccg gttgaaatcg ctccgcttgt
      361 tgtaatcgag cgagcctccc acggtgaagt tgatcgaagg tgtggtggcg atatcgaatt
      421 tcacacttcc caggtacgtg cggctggcag cgttctggcg ggtcttgatc cgctcaacgt
      481 catcggcctt gaggtagttg ctccggtaca cggccgccac gtcgttcccg atcacgcgga
      541 cagccatcgg attgcgcacc agatcgtcgc gcacgtcctt cttcacacgc aggtcgccgc
      601 catagagggg gccgccatcc accgcatcgg tgaactggcc gctgaggaag aagcccagca
      661 agggccgcgt tttgttgccc gcgctgtcct ttttgaagaa gacggggccg ctgaggctgg
      721 cctccacctg gttgaaggca tacttgtcca agccgacgat gtccgtgaca tcttcgccca
      781 ccttccaacc actggacaga tactccaatc caccgaagaa tgtgcggccc ggaccgcgcg
      841 tggtgatgtt caccagaccg cccgtaacgt cgccgtagtt ggccggcaca ccaccggtca
      901 ccacctgcac ttcctcgatc gcgctcttgg gcagaccggt accggcacct gcgggcactt
      961 tcacaccgtc gatatagtag taggtgttct cggatcgcgc cccgcggatg ctgatgccgc
     1021 cgccggtgcc ggccgtactg gccccggcca ccgtggtggc aacaccttgg gccgagcggg
     1081 ccggcatgcg ggcgatctgc tcacgcgtca ccgtgctgcc gctggccccg ccatccttgt
     1141 cgatcagggg cactttgtaa cggaccactt caacctcctt caggtccact ccgctggtga
     1201 gctcaggctc caggaaggtg atcttgttgc tgttgatgat cacgcccgtt tgcttcatgg
     1261 gctgatagcc cacgtaactc accgttacat cgtaggtgcc gggttcgatg ggaccgatct
     1321 tgaacaagcc atcgaagtcc gtttgacctc cggccacgcg cgtgccgcgg ttctccacca
     1381 ccacactcac gaagggcagg gcttctccgg tctttttgtc cttgacggtg ccctgaaggg
     1441 tgcccgagcc aacctgcgcg aatgcggagc tggtggcgag cacaacaagg gtcagggccg
     1501 agagtaggcg gctcagcata aattcaagtg tttgcgttgt tcttcttggc gacgaggggg
     1561 cgctaatata gaaacaagca cgatatgtga aaatgtctga aatccttgct ggacgcgggt
     1621 ttccaaagcg atttctacgg atggggcccc gaacgggaag gccagggggg gtacaggttg
     1681 gtccgttcgc atggatccac tggagagcgt atccacaact cctttgtgat ccgtgttcaa
     1741 tgaagcaaca ggctggcaac atccttggcg atcggaggag gagggatccg cttcgcgatg
     1801 gtcggtcatc agcctacgga tacgaagaac cttggaggaa tttcgcggtg atatgcaggt
     1861 cgtgcgctcc atcgcctttg ttaacgaacg ctaagaaagt gcaaagggag ccgatccgta
     1921 ggccaggaac gagcaacggc ggtccggcac aagggaacgg gaataaccgt gatcagtgct
     1981 tgcgaaaaag ccgctggagg aaagggtcgc ggtggccacg cggaccgttg cggtccgatg
     2041 agcgcttgtt ccgtttcatc cgtttgcgcg tggccttgtc ctgatgctga cgatgctgct
     2101 tgagcaggcg cttctcctcc ttggcgagcg acttcttgtc cttgcgttcc ttcttggcca
     2161 agttcctctc ctgctgtttg cgcgaaatgc ccgcactgtt ccctccacca tcgccgtcct
     2221 gggcgtgggc caccaaaggc aaggtgagga gaaccgcgaa catgaacacc gcacggaaca
     2281 agcgcatcat ggggcgaagt tcgtggctgc ggggcatgtt cccatgcacg ggtaccgatc
     2341 aaggcggaca aacgttgtac acccgcggcc acccgggccc gttaccttcg taccatgaac
     2401 cggttcctgc tgttctcatc gctcgtgatc gccctgctct ccgtatcctg taaaaaggaa
     2461 agcacgacgg gtgtgccgcc cgtacagctg gacgtaagta tcaatatcaa cctgccggaa
     2521 tacgccgacc tgcaagtggt ggggggctgg gtgtacctga ccggaggctc gcaggggctc
     2581 attgtctacc gcaagaacca ggacgaattc tccgcgctcg accggcactg ccccttccag
     2641 ccggagaacc tctgccatgt gatcgtggac gagagcggag tgatcgcacg agacacggcc
     2701 tgttgccatt cggcctacct gatcatggat ggccaaccca ccgaaggtcc gtccagcttc
     2761 ccgctcaagg cgtaccacac gaccttcaac ggtacgatgc tgcggatcta taactgagga
     2821 agcgacgagt ggcgagtggc gagtggcgag agacgagtga caacatactg tctcgcctct
     2881 cacctctcgc ctctcgtctc ttgtttctca gtagcggtac gcgtcgctct tgaagggacc
     2941 gttcttcggc acaccgatgt acttggcctg ggcttcggtg agttcctcca gctccacgcc
     3001 gatcttggcc aggtgcagac tcgccaccat ctcgtcgagg tgcttgggca gcacgtacac
     3061 cttgttctcg tagttcttgt ggttcttcca cagctccagt tgcgcgagcg tctggttggt
     3121 gaagctgttg ctcatcacga agctggggtg gcccgtggcg caacccaggt tcaccaggcg
     3181 accttcggcc aggatgatga tgtccttacc gtcgatcgtg tatttgtcca cctgcggctt
     3241 gatctccacc ttggtgctgc cgtaggcctt gttcaaccac gccatatcga tctcgttgtc
     3301 gaagtggccg atgttgcaca cgatggtttt gtccttcatc ttgcggaagg cggcctcggt
     3361 aac
//
LOCUS       DAOQOC010000185         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_13996_length_3354_cov_3.315550, whole genome shotgun sequence.
ACCESSION   DAOQOC010000185 DAOQOC010000000
VERSION     DAOQOC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13996_length_3354_cov_3.315550"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..532)
                     /gene="rpsM"
                     /locus_tag="PL106_04080"
     CDS             complement(155..532)
                     /gene="rpsM"
                     /locus_tag="PL106_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNA32280.1"
                     /translation="MARIAGIDLPKSKRGAIGLTYIYGIGRSTALTILEKAGVDPDTK
                     VEDWTDEDQGRIRTFINENIKTEGALRSEVQLSIKRLVDINSYRGQRHRNGLPVRGQR
                     TRTNSRTRKGKRKTVANKKKATK"
     gene            complement(576..692)
                     /gene="ykgO"
                     /locus_tag="PL106_04085"
     CDS             complement(576..692)
                     /gene="ykgO"
                     /locus_tag="PL106_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNA32281.1"
                     /translation="MKIRASIKKRSADCKLVRRKGRLYIINKKNPKFKQRQG"
     gene            complement(717..938)
                     /gene="infA"
                     /locus_tag="PL106_04090"
     CDS             complement(717..938)
                     /gene="infA"
                     /locus_tag="PL106_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNA32282.1"
                     /translation="MSKTGNIEQDGVIKEALSNAMFRVELENGHVIVAHISGKMRMHY
                     IRILPGDKVKVEMSPYDLSKGRISFRYKT"
     gene            complement(966..1784)
                     /gene="map"
                     /locus_tag="PL106_04095"
     CDS             complement(966..1784)
                     /gene="map"
                     /locus_tag="PL106_04095"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNA32283.1"
                     /translation="MGKAVSILSDAEIGCVRESSLLVGRTLAEVAKRIRAGVTTAELD
                     ALAEQFIRDHGALPGFKGLYDCPSTLLISVNEAVVHGLPGDRELRDGDIASVDCGVLM
                     NGFYGDSAYTFCVGEVAEETRSLLRVTQECLALGIEQAVVGNRVGDIGFAVQSHAEKN
                     GYGVVRELTGHGVGRKLHEGPEVPNFGRRGHGAKLQEGMALAIEPMINMGSREARQLE
                     DGWTVVTLDGKPSAHFEHTVAVRKGKAEVLSTFSYIEDVLKQKGEWVGTATFPA"
     gene            complement(1787..3121)
                     /gene="secY"
                     /locus_tag="PL106_04100"
     CDS             complement(1787..3121)
                     /gene="secY"
                     /locus_tag="PL106_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNA32284.1"
                     /translation="MKGLLETFKNIWRIEELRKRILITLGLLLVYRIGSFIVLPGVDS
                     ARLAGSSSGGSGLVDLLSIFTGGAFTRASIFALGVMPYISASIIMQLAGIAIPAVQKM
                     QREESGRRKINQWTRYITIIISLFQAPGYILTYVDAEARPVNDPMWMPTSVVILTCST
                     LFIMWIGERITERGLGNGVSLIIMIGILARLPESFIQEFVSRMGGGGGLVAVLMEVAF
                     WLLIIAGNILLVQGTRRIPVQFAKRVVGNKQYGGVRNYVPLKVNAAGVMPITFAQAIV
                     LIPMYLTQIPALRDVQWMARFSDYKSVGYNITLFLMVVAFTYFYTAITVNPNQLADDM
                     KRNGGFIPGVKPGKATAGHIDELLSRITLPGAIFLGIVAILPALAGGVLGIKDGFAQF
                     FGGTSLLIMVGVLLDTLQQIESHLLMRHYDGLMKTGRIKGRQSATYSSMAAG"
     gene            complement(3124..>3354)
                     /locus_tag="PL106_04105"
     CDS             complement(3124..>3354)
                     /locus_tag="PL106_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HNA32285.1"
                     /translation="YKGINLDAVQALADKNKGVVDIAALRANGLIGKNDLVKILGRGE
                     LTGAIDVKANAFSASAKAAIEAKGGKVEIVNR"
BASE COUNT          575 a         1068 c          953 g          758 t
ORIGIN      
        1 gacgatcagg ttgttgaagg tcgcgcggat gtgggcctgc ccgaaggcct ccacgaccac
       61 gttcttcttc ttcttcttga cggcggcacc gcccttttcc tgctgttttg ccattgtctc
      121 gtcgtattcg gtctacgccc gcgtgaaccc tgcgttattt cgttgctttc ttcttgttgg
      181 ccacggtctt gcgcttgcct ttgcgggtgc ggctgttggt gcgcgtgcgt tggccccgca
      241 cgggcagccc gttgcggtgg cgctggccac ggtagctgtt gatgtccacc aagcgcttga
      301 tgctcagttg cacctcgctg cgcagcgccc cttcggtctt gatgttctcg ttgatgaagg
      361 tacggatgcg gccctggtcc tcatcggtcc agtcctccac cttggtatcg ggatccacac
      421 cggccttctc cagaatggtg agggcggtgc tgcgtccgat gccgtagatg taggtgaggc
      481 cgatggcccc gcgcttgctc ttgggtaggt ctatgcctgc gatacgtgcc atgctttgaa
      541 cgccgtagcc cggcgtaggt gttgtcgtgc gtttatcaac cctgacgttg cttgaacttc
      601 gggttcttct tgttgatgat gtacaggcgt cccttgcgac ggaccagctt gcagtccgcc
      661 gaacgcttct tgatggaggc cctgatcttc atgggttcct ttgttgttct tgttcgtcag
      721 gtcttgtacc ggaaggagat gcggcccttg ctcagatcgt acgggctcat ctccactttc
      781 actttgtctc ccggcaggat ccggatgtag tgcatccgca tcttgccgct gatatgcgcc
      841 acgatcacgt ggccgttctc gagttcaacc cggaacatgg cattgctcag cgcctctttg
      901 atgacgccgt cctgctcgat gttcccggtc ttgctcattc gttcgtgctt ctcttttcct
      961 atccgttatg cggggaacgt tgccgtcccc acccattcac ctttctgctt cagcacatct
     1021 tcgatgtagc tgaaggttga caggacctcc gccttgccct tgcgcaccgc cacggtgtgt
     1081 tcgaaatgcg cgctcggttt gccatcgagc gtcaccaccg tccaaccgtc ctccaactgc
     1141 ctggcctccc tgctgcccat gttgatcatg ggttcaatgg ccagggccat tccttcctgg
     1201 agtttggcgc catgcccgcg acgtccgaaa ttggggactt cggggccttc gtgcagtttg
     1261 cgtcccacgc catggccggt caattcgcgc accacgccgt atccgttctt ctcggcatgc
     1321 gattgcaccg cgaaaccgat atcgcccacg cggttgccga ccacggcctg ctcgatcccg
     1381 agcgccaggc attcctgggt cacgcgcaac agactccggg tctcctcggc cacctcgccc
     1441 acacagaaag tgtaggcgct gtcaccatag aagccgttca tcagcacccc gcaatccaca
     1501 ctggcgatgt ccccatcgcg caattcgcga tcaccgggga ggccatgcac cactgcctcg
     1561 ttcacgctga tcagcagcgt gctcggacaa tcgtaaagtc ccttgaatcc cggtagcgca
     1621 ccgtggtcgc ggatgaactg ctcggccagt gcatccaatt cagccgttgt gacgcccgca
     1681 cggatgcgtt tcgccacttc ggccaaggtc ctgccaacaa gcaaagaact ctccctcaca
     1741 cacccgatct ccgcatcact gaggatgctc accgccttcc ccatggctac ccggccgcca
     1801 tgctgctgta cgtcgcgctc tgcctaccct tgatacgacc ggtcttcatc aggccgtcgt
     1861 aatgccgcat caagagatgg ctttctatct gttggagcgt gtccagcagc acgcccacca
     1921 tgatcaacaa tgaagttccg ccgaaaaact gcgcgaagcc gtccttgata ccgaggaccc
     1981 cgcccgcaag tgccgggagg atggccacga tgcccaggaa gatggcaccc ggcagggtga
     2041 tgcgactgag cagttcgtcg atgtgcccgg cggtggcctt gcccggcttc acgcccggga
     2101 tgaacccgcc attgcgcttc atgtcgtccg ccaattggtt ggggttgacc gtgatggcgg
     2161 tgtagaagta ggtgaaagcc accaccatga ggaagagggt gatgttgtag cccacgctct
     2221 tgtagtcgct gaagcgggcc atccattgca catcccgaag tgcggggatc tgggtcaggt
     2281 acatggggat gagcacgatg gcctgggcga aagtgatggg catcacaccg gctgcgttca
     2341 ccttgagggg aacgtagttg cgcacaccgc cgtactgctt gttgcccacc acgcgcttgg
     2401 cgaactgcac cgggatgcgg cgggttccct gcaccagcag tatgttaccc gcgatgatca
     2461 acagccagaa ggccacttcc atgaggacgg ccaccagacc gccaccaccg cccatgcggc
     2521 tcacgaactc ctgtatgaag gattcgggca ggcgtgccag gatgccgatc atgatgatca
     2581 aggagacccc gttaccgagc ccgcgctcgg tgatccgctc gccgatccac atgatgaaca
     2641 gggtgctgca ggtcaggatg accacactcg tgggcatcca catcgggtcg ttcacgggcc
     2701 gggcttcggc gtccacatag gtcaggatgt aacccggagc ctggaagagg ctgatgatga
     2761 tggtgatata gcgcgtccac tggttgatct tgcggcggcc actttcctcg cgttgcattt
     2821 tctgcacggc ggggatggcg atcccggcga gctgcatgat gatgctcgcg ctgatgtaag
     2881 gcatcacacc gagagcgaag atgctggccc gtgtgaaagc gccacccgtg aagatgctca
     2941 gcaggtccac cagcccggag ccgcccgagc tgctgccggc cagtcgcgcg ctgtccaccc
     3001 cgggcagcac gatgaagctg ccgatcctgt acaccagcaa cagccccagc gtgatgagga
     3061 tacgcttgcg caattcctcg atgcgccaga tgttcttgaa tgtttcgagc agccccttca
     3121 tgatcaacgg ttcacgatct ccactttacc tcccttcgct tcgatggcag ccttggcgga
     3181 tgcgctgaag gcattcgcct tcacgtcaat ggcacccgtg agctcaccgc gacccaggat
     3241 cttcaccagg tcgttcttgc caatgagtcc gttcgcacgc aaggccgcga tgtccaccac
     3301 gcccttgttc ttgtcggcaa gggcctgaac agcgtccaag ttgatgccct tgta
//
LOCUS       DAOQOC010000186         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14045_length_3348_cov_4.016398, whole genome shotgun sequence.
ACCESSION   DAOQOC010000186 DAOQOC010000000
VERSION     DAOQOC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14045_length_3348_cov_4.016398"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PL106_04110"
     CDS             complement(<1..855)
                     /locus_tag="PL106_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32286.1"
                     /translation="MLPSQRTIHLLDKARSAYSRLFPGKSRFGFSYDLAQLRGQEGCD
                     RIREELLAKKPSMICRFGSTELLSLLGYLGHLADPKPSLLNHIRYITGALPGYGHDPE
                     HTRFLYMLSGVFPPRPDVEQRFGARMVADMELVDILGTWRREETFFAKELAHAVKVEL
                     PNLEPFLYNNPWSEVLEGKTVLVVHPFERTILSQFQKREKLFKDPRVLPPFELKTIRA
                     VQTVAGNPTSFKDWFEALGYMEDRMSGMDWDVAIIGCGAYGFPLAAHAKRMGRKAVHI
                     GGATQLLFG"
     gene            1049..>3348
                     /locus_tag="PL106_04115"
     CDS             1049..>3348
                     /locus_tag="PL106_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNA32287.1"
                     /translation="MMRPVPKRDLLGYVCALFLVALFAPAAEATVYYVSPTGNDTNNG
                     TSQASAWRTIDRVNQSAYTIQPGDQILFQRGGHYRGGIIWGTSGAAGAPITYGAYGTG
                     ADPIIDGARLVTGWTPYQGNIWRAQVGTEVSQVYVGGNRSILARTPNTGWFRNDQGSG
                     NTMHSNDLTQSNGFWTGARCILRCTASSYDTLRVAAFNNGTLTFSTQPTNTNMAGDDW
                     GFYLDKRLDLLDASNEWYYEPSTGYLYLQAPGNADPNGLAVEACTEWAGLLCSYQRHH
                     MVAENLVFRHHRNAGVRVDDASFVTIRNCVIEDTYHGIRSYGHDNLFEHNTISRTLAT
                     GCLMIDNNSVFQWNDLEDIATIPGEGETQWGYFGIRGIGQNNVIRGNRLENIGYIGIT
                     ANGNHLIEKNTIHHYLTTLNDGGAIAFDHSDGLIVQDNIMYDAVCGLDGSSTVLPHYQ
                     EMGLGIYFGNTSNVNTTVQRNTIANLSGVGINVDHNMNTHGHQVRDNVIFNCGIGMSI
                     SDYSNSNGPGAQFPYYVANYDDQYSNNVIYGLRKDQYALRFYNCYSAQPTDFGTFTNN
                     RYFNPYNEMAIFLFSFQAGQHWFSLEHWQAARNEEAGTSRSPLRLSEWTTVNELSGEL
                     IPSGSFNGSSNGWANAVWPNNAQVTHDVTHLDQGCLKVYIPDNSVYGECSVRGPEWFP
                     IENGPDDWYRLDLSLQGDQEGYLKCAIKGESQSGNPYTRYDRLLPFGPGRRDRSIYFQ
                     VSASEQAQLLLTNKWTLPQYYIDNIHL"
BASE COUNT          711 a         1027 c          978 g          632 t
ORIGIN      
        1 accgaaaagc agttgcgtgg ccccgccgat atgcaccgcc ttacggccca tgcgcttggc
       61 gtgcgccgcc aggggaaatc cgtacgcgcc acagccgatg atggccacgt cccaatccat
      121 gccgctcatg cggtcctcca tgtagcccag cgcctcgaac cagtccttga aggaggtggg
      181 attgccggcc acggtctgca cggcacggat ggtcttcagt tcgaaaggag gcagtacgcg
      241 agggtccttg aacagcttct cccgcttctg gaactggctc aggatggtgc gctcgaacgg
      301 atgcaccacc agtacggtct tcccttccaa tacttcactc cagggattgt tgtaaaggaa
      361 tggttcaagg ttgggcagct ccactttcac ggcatgggcg agttccttgg cgaagaaggt
      421 ctcctcgcgc cgccatgtgc ccagaatgtc caccagttcc atgtccgcca ccatgcgcgc
      481 cccgaagcgt tgctccacat cgggccgtgg agggaatacc ccgctgagca tgtacaggaa
      541 tcgggtgtgc tccgggtcgt gcccgtagcc cggtagtgca ccggtgatgt agcggatgtg
      601 gttgagcagg gagggcttgg ggtcggcaag gtggccgagg taccccagca gggagagcag
      661 ttcggtactg ccgaagcggc agatcatgct gggcttcttg gccagcaact cctcacggat
      721 gcggtcgcag ccctcctggc cacggagttg ggccaggtcg tacgagaaac cgaatcggct
      781 tttaccggga aacaagcgcg aatacgcgct acgggccttg tccagaagat ggatcgtgcg
      841 ttgactgggc agcatcgggg ggcttggtac ggtgccggac cgggtgaagt tcgttggtgt
      901 gagggccggt tctccaggcc gcgaaattac ctgcgccgaa agtgctcacc gacggatcgg
      961 ggctttcgtc gaaatgggac cgggcctggt cgaaaatgcc gaacggcttc ccactcgtcg
     1021 tggttgaaag cgcaaaaccg actggatcat gatgcgtcct gtaccaaagc gcgaccttct
     1081 cggatacgtg tgtgcacttt tcctggtggc ccttttcgct cctgcggcgg aggctacggt
     1141 gtattacgtc tctcccaccg gcaacgacac caataacggt acatcccagg cctctgcttg
     1201 gcgcacgatc gaccgggtga accagagcgc gtacaccatc cagccaggcg accagatcct
     1261 gttccaacgc ggcggccatt accgtggtgg catcatctgg ggcacctcgg gggcggctgg
     1321 cgccccgatc acatacggcg cctacggtac cggcgccgat ccgatcatcg atggcgctcg
     1381 cctggtgacg ggctggaccc cgtaccaagg gaacatctgg cgtgcacaag tcggaaccga
     1441 ggtgagccag gtgtatgtgg gcggtaaccg gtcgatactg gcccgcaccc ccaacacggg
     1501 ttggttccgg aacgaccaag gcagcggcaa cacgatgcac agcaacgacc tcacccaatc
     1561 caacggtttc tggaccgggg cgcgctgcat cctgcgctgc acggcaagca gttacgatac
     1621 actgagggtc gcggccttca acaacggtac gctcaccttc agcacccagc ccaccaatac
     1681 caacatggcg ggcgatgact ggggctttta tctggacaag cggctcgatc tgctggatgc
     1741 gtccaacgaa tggtattatg aaccctccac aggatacctg tacctgcaag ctcccggtaa
     1801 tgccgacccc aatggccttg cagtggaggc ctgtacggaa tgggccggcc tgctctgttc
     1861 ctaccagcgg caccacatgg tggccgagaa cctcgtcttc cggcatcacc gcaacgccgg
     1921 tgtacgcgtg gatgacgcca gtttcgtcac catcaggaac tgcgtgatcg aggatacata
     1981 ccatggtatt cgtagctatg gtcacgataa cctgttcgag cacaacacca tcagccgcac
     2041 actggccacg ggctgcctga tgatcgacaa caattcggtc ttccaatgga atgatctgga
     2101 ggacatcgcg accatccccg gtgaggggga gacccagtgg ggctacttcg gtatccgggg
     2161 tatcggccag aacaacgtga tccgtggcaa tcgcctggag aacatcggct acatcggcat
     2221 caccgccaac gggaaccacc tgatcgagaa gaacaccatc catcactacc tgacgacgtt
     2281 gaacgatggt ggggccatcg cattcgacca ttcggacgga ctcatcgtgc aggacaatat
     2341 catgtacgat gccgtttgcg gtcttgatgg ctcttccacc gtgttgccgc attaccagga
     2401 aatgggcctg ggcatctact tcggcaacac gagcaacgtg aataccaccg tacaacggaa
     2461 caccatagcc aacctgtccg gggtgggcat caatgtggat cacaacatga acacgcacgg
     2521 ccatcaggtg cgcgacaacg tcatcttcaa ctgcggcatc ggcatgtcca tcagcgacta
     2581 ttccaacagc aatggccccg gtgcgcaatt cccgtactat gtggccaact atgatgacca
     2641 gtactccaac aatgtgatct acggccttcg caaggaccag tacgcgctgc ggttctacaa
     2701 ctgctacagt gcgcagccca ccgatttcgg caccttcacg aacaatcgct acttcaatcc
     2761 ctacaacgag atggccatct tcctgttcag cttccaggcg gggcagcact ggttcagcct
     2821 ggagcattgg caggccgccc ggaacgagga ggcgggcacc tcgcgcagtc cgctccgcct
     2881 gagcgagtgg accacggtga acgaactgtc cggcgagctc atcccgagcg gttcattcaa
     2941 cggttcctcg aacggatggg ccaacgctgt gtggccgaac aatgcccaag tgacccacga
     3001 cgtcacgcac ctggaccaag gctgcctgaa ggtgtacatc cccgacaatt cggtctacgg
     3061 cgaatgttcg gtacgcggtc ccgaatggtt cccgatagag aacgggccgg acgattggta
     3121 ccggctggac ctcagcctgc aaggcgacca ggaggggtac ctcaaatgtg ccatcaaggg
     3181 tgaatcacag tcggggaatc cctacacccg ctatgatcgg ttgctgccat tcggccctgg
     3241 ccgacgggac aggagcatct atttccaggt atccgcctcc gaacaggccc agctcctgtt
     3301 gaccaacaaa tggacgttgc cgcagtacta tatcgacaac atccatct
//
LOCUS       DAOQOC010000187         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14050_length_3347_cov_3.889429, whole genome shotgun sequence.
ACCESSION   DAOQOC010000187 DAOQOC010000000
VERSION     DAOQOC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14050_length_3347_cov_3.889429"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PL106_04120"
     CDS             <1..1268
                     /locus_tag="PL106_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32288.1"
                     /translation="GVVNGTATIDACGICSGGTTGITPNSTCTDCAGVVNGTASIDAC
                     GICSGGTTGINPNSTCTDCAGVVNGTATIDACGTCSGGTTGITPNSTCTDCAGVVNGT
                     ATIDACGICSGGTTGITPNSTCTDCLGVVNGPALPGSTCDDGDPDTENDQWQVNCTCI
                     GTPVNPCNPPTANAGPDATVCALSTMLNAVADVVPGGWSCASPSVAFSNVNDPTTVVS
                     ATSPGTYTCVWTVGNPDCWSSDTAIITLLDPAVPEWVDAGDDQTLDSRTFTDLQGSAP
                     TGVIISWSVLSGTGEFSDATEAQCTVTGLSIGMNILKLEAMYPNGCASASDTMAITVN
                     DIFIPEAFSPNGDGANEALEITGILEYPRSELLVFDRWGQEVLRRTGYTNDWIGTARN
                     GQPLPDDTYFYVLNLDGERTYNGFFVIKR"
     gene            1268..2191
                     /locus_tag="PL106_04125"
     CDS             1268..2191
                     /locus_tag="PL106_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA32289.1"
                     /translation="MTGPRYIQLTLALLAFMGARGQHTPLTSQYLFNGLLINPAYAGS
                     RDAFTANLTWRRQWAGFKGAPETQILSLHSPIRNSRVSAGVLLVNDRIGVSRETALLT
                     NYAYRLPIGRGTLQFGLGAGVSLLQANWTSLAVQDRNDAQFAVDTRSAFRPNFSTGLF
                     YYQKRFFVGASLPFALHHSFNVERQAWTMSSALKDQQPMLTGGYVFRIRKDLQLKPST
                     LIRYQSATGIQADLSANLIFRDRIWTGLSYRSNDAITGSFEVLPTPQWRIGYAYDLSI
                     SRLLPYHFGSHEVMVQYEFGKRIRVRNSRYF"
     gene            2195..>3347
                     /locus_tag="PL106_04130"
     CDS             2195..>3347
                     /locus_tag="PL106_04130"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32290.1"
                     /translation="MKRPAILIPTTVIALASLAQETTVVEPVRLYPAGMDNCPVLLDS
                     TVVISSMRERPSLAPVRAYDTNEPFSDLYAFDWNGQQASSPRLFSEALCGPLNDGPAT
                     FGPGGATICFTRNQSDVKGRTRKDGDRLGLFFSTLKDGEWTTPVPFDYNSGAYNVMHP
                     SLSPDGRTLIFASDMPGGYGGADLFKCELEDGAWSIPLNMGPEVNSPSHELFPCVSAN
                     WKLRFASDREGGLGKLDLYVCAPDGPGWKRPEALPAPMNSTGNDYGYTSFLTDRSGFL
                     TSDRDGSDRIYQFRRMVPLFTDCREQREISYCYAFAEPRLSQANGLPVHPRWDLGDGT
                     VIDGPEVRHCYAGPGRYKVSLDLVDNTSGHVFFHQNDQELVIQDIPQPYI"
BASE COUNT          688 a         1067 c          955 g          637 t
ORIGIN      
        1 cgggtgttgt gaacggcacg gccaccatcg acgcctgcgg catctgctcg ggcggaacca
       61 ccggaatcac tcccaatagc acctgcacgg actgtgccgg tgtcgtgaac ggcacggcca
      121 gcatagacgc ttgcggcatc tgctcgggtg gaaccaccgg catcaacccc aacagcactt
      181 gtaccgattg cgcgggtgtt gtgaacggta cggccaccat cgacgcttgc ggcacctgct
      241 cgggtggaac caccggcatc acgcccaaca gcacctgtac cgattgcgcg ggtgttgtga
      301 acggcacggc caccatcgac gcctgcggca tctgctcggg cggcaccacc ggcatcactc
      361 ccaatagcac ctgcacggat tgcctgggcg ttgtgaatgg tcctgcattg ccgggatcca
      421 cttgcgacga cggagacccg gatacagaga acgatcagtg gcaggtcaac tgcacctgca
      481 tcggcacacc cgtgaacccg tgcaacccgc caaccgcgaa cgcgggtccc gatgcgaccg
      541 tgtgtgcatt gagcaccatg cttaacgccg tagccgatgt ggtgcccgga ggttggagtt
      601 gcgcgagtcc ctccgtggcc ttcagcaacg tcaacgatcc gacaacagtg gtatcagcca
      661 cctcaccagg cacgtacacc tgcgtgtgga ccgtgggcaa cccggattgt tggtcctcgg
      721 acacggcgat catcaccctc ctggatcctg cggtgcctga atgggtggat gcaggagacg
      781 accagaccct cgactctcgg accttcaccg acctgcaagg gtcagctccg acgggtgtga
      841 tcatctcctg gagtgtcctc agcggaactg gcgaattctc cgatgccact gaagcccagt
      901 gtaccgtgac cggtctgtcg atcgggatga acatcctgaa gctcgaggcc atgtacccga
      961 acggttgtgc cagtgcgagt gataccatgg cgatcacggt caacgacatc ttcattccgg
     1021 aggcgttctc gcccaacggc gatggagcga acgaggccct tgagatcacc gggatcctgg
     1081 aatatccgcg cagcgagctc ctcgtattcg atcgctgggg ccaggaggtc ctgcgtcgaa
     1141 cgggctatac caacgactgg atcggcacag ccaggaacgg ccaacccctc ccggatgaca
     1201 cctacttcta tgtcctgaac ctcgatggtg aacgcacgta caacgggttc ttcgtgatca
     1261 aacgctgatg acagggccgc gatacataca gctcaccttg gcgctcctgg ccttcatggg
     1321 ggcgcgtggc cagcacacgc cgctcaccag ccagtacctc ttcaatggcc tgttgatcaa
     1381 cccggcgtac gctggtagca gggatgcctt cacggcgaac ctgacctggc gtcggcaatg
     1441 ggccggattc aagggggctc cggaaacaca gatactcagc ctccattccc cgatcaggaa
     1501 ttcgcgcgtg tccgcgggcg tgctcctggt gaatgatcgc atcggagtga gccgtgaaac
     1561 cgccctgctc accaattatg cctatcgcct gcccataggg cgcggcactc tgcagttcgg
     1621 tctcggtgcc ggcgtatcgc tcttgcaggc caattggaca agcctcgctg tacaggaccg
     1681 caacgacgcc caattcgcag tggacacgcg aagcgctttc cggccgaact tcagcaccgg
     1741 gctgttctac taccagaaac gcttcttcgt cggtgcctcg cttcccttcg cgttgcatca
     1801 cagcttcaac gtggagcgcc aggcgtggac catgagttcg gcgctcaagg accaacagcc
     1861 catgctcacg ggcggttacg tcttccggat ccgcaaggat ctccaactca aaccgagtac
     1921 cttgatccga tatcaatccg cgacggggat ccaggcggac ctcagcgcca acctgatctt
     1981 ccgcgaccgc atctggaccg ggctcagtta caggagcaac gatgccatca ccggaagttt
     2041 cgaggtgctc cccacgccgc agtggcgcat cggatacgcc tacgacctca gcatctcccg
     2101 tctccttccc tatcacttcg gatcccacga ggtgatggtg cagtacgaat tcggcaaacg
     2161 catccgggta cggaattccc gttacttctg atccatgaag cgacccgcaa tattgatccc
     2221 gacaacagtg atcgcgctgg cctcgttggc ccaggagacc acggtggtgg aacccgtgcg
     2281 gttgtatccc gcaggcatgg acaactgccc ggtactcctg gacagtaccg tcgtgatcag
     2341 ttccatgcgc gagcgtccat cactcgcccc ggtgcgtgca tatgatacca acgaaccgtt
     2401 cagcgacctg tacgcgttcg attggaacgg tcagcaagcc tcctccccac gactgttcag
     2461 cgaagcactc tgtggcccgc tcaatgacgg tccagccacc ttcggccctg gtggggcgac
     2521 catctgcttc acgcgcaacc agagcgatgt gaaaggtcga acccgaaagg atggtgatcg
     2581 cctcgggctt ttcttctcca cgttgaagga cggcgaatgg acgacacccg taccattcga
     2641 ctacaacagt ggcgcgtaca acgtgatgca tccgtcgttg agccctgatg gccgcacact
     2701 gatattcgcc agtgacatgc ccggcggata tggtggtgcg gacctgttca agtgtgaact
     2761 ggaggatggt gcctggtcaa tcccactcaa tatgggacct gaagtgaaca gtccatcaca
     2821 tgaattgttc ccctgtgtat ccgcgaactg gaagctccga ttcgccagcg atcgggaggg
     2881 tggcttgggc aaactcgacc tgtacgtatg cgcacctgac ggtccgggat ggaaaaggcc
     2941 cgaagcactg ccagcaccga tgaactcgac cgggaacgat tacggataca cgtccttcct
     3001 taccgaccgc agtggcttcc tcaccagcga tcgcgatggg agcgaccgga tctaccagtt
     3061 ccgcagaatg gtaccgctct tcaccgattg ccgggagcag cgcgagatca gctattgcta
     3121 tgctttcgcg gaaccccggc tcagtcaggc caatgggcta ccggtgcatc cacgctggga
     3181 cctcggcgat ggcacggtga tcgatgggcc ggaggtacgg cattgttacg caggacctgg
     3241 ccgatacaag gtgtcccttg acctcgtgga caacacgagc ggacatgtct tcttccacca
     3301 gaacgaccag gaactcgtta tccaggacat cccacagccg tacatca
//
LOCUS       DAOQOC010000188         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14066_length_3345_cov_4.068693, whole genome shotgun sequence.
ACCESSION   DAOQOC010000188 DAOQOC010000000
VERSION     DAOQOC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14066_length_3345_cov_4.068693"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..908
                     /locus_tag="PL106_04135"
     CDS             159..908
                     /locus_tag="PL106_04135"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32291.1"
                     /translation="MRTFISVAVFGLMNLALNAQPTLDITLAGIGDNAYEVRVRPDAF
                     FDGVFSSLVFTLRWSEGDGIGPVGLTSAPDAPISASPSGTQRNDGLYNYRSYAGFGFT
                     PLVDPDQQWRPGVEHVLLTFTFNGPGQVELVNDPWTASNNGDYYVSLNGYDRTGIIYQ
                     DVSTNVGPMKQDPAMVSCWPNPATDIVHCRINGITPGSAVNVLDGLGRIVATTRTDGS
                     NDPSITIGHLAAGTYSLRLASGAQVGSFIKR"
     gene            1154..2326
                     /locus_tag="PL106_04140"
     CDS             1154..2326
                     /locus_tag="PL106_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32292.1"
                     /translation="MIRPSADSFAQATPISAHEDVRRYAEVIRQEHVDPFTCVSRQHF
                     DSCAAALDERLSNEDRTGLLIGLLRLTAMLNDEHTMVRWNARLRLPIQVHPLDEDLCI
                     IGTDSAHRPLLLHRVIAINDVPASSVLERFAALVKQDNPSYAAAWLGWNLCDVDLLMG
                     LGIVTDPEHVRLGLLDMEGDTVSAEVHSIDAQAVQWVRPASMCLQWASRVKGNYGSLF
                     DDSTSTLYVNYRRCREDENISMSAFTEEVFARIEAQHPARLVIDLRENGGGSSALFMP
                     FIKRLRKGYLNKENRIRVLIGRGTFSSAVMNAVELKRTTSAVLIGEVTGGNVNHYGEV
                     REEALPWSGFKVLYSTKHWVNWPGVVGGLKPDEYVGPSIADVVNGSDAAIERAIDR"
     gene            2583..>3345
                     /locus_tag="PL106_04145"
     CDS             2583..>3345
                     /locus_tag="PL106_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA32293.1"
                     /translation="MLFVHGTPDWNLGSRHPIKAFSATHRGVAVVHPGFWISDKPADA
                     DCMVAAHTRRLQGLIDKLDLKDLTLVVTDFGGGIGLQHALERPCNVKHVVLYNTWMWD
                     LMPDKRFARPARIMNSWLGRFLLSAHGFTVNMMMPNAYGDKSRLTPRIHAHDKNALPD
                     ASSRAANFACVQEMANAGPFWNAQWEQVDRSRNMRVLSCWGMKDGFLPNDLLPWWKQA
                     FPDAAVSAFPHPGKSCKGKRTRSWWPRCGRSSPGST"
BASE COUNT          670 a          985 c         1010 g          680 t
ORIGIN      
        1 ccgccaatac cgttaccggc tatgcggcca ctgatgtgaa catggacgga gtgaccaagt
       61 acaccggcgc ggggaacgac cgggatccca tcctcgccaa catcgggggc accgtaccct
      121 cgaacgtcct caccgaacaa cttccttgaa ccatcaccat gagaaccttc atttcagttg
      181 cagtcttcgg gctgatgaac cttgcgctca acgcacaacc caccttggac atcacacttg
      241 ccgggatcgg tgacaatgcc tacgaagtgc gcgtgcgccc cgatgctttc ttcgatggtg
      301 tgttcagttc gttggtgttc acgttgcgtt ggagcgaagg tgacgggatc ggccctgtgg
      361 gactgacctc cgcgcccgat gccccgatca gcgcctcgcc gagcgggaca cagcggaacg
      421 atggcttgta caactaccgc agctacgcgg gcttcggctt cacaccgctg gtggatcccg
      481 atcagcaatg gcggccgggt gtggagcacg tgctgctcac ctttaccttc aacggacccg
      541 gccaggtgga attggtgaac gatccttgga ccgcatcgaa caacggcgac tattacgttt
      601 cattgaacgg ctacgaccgc acgggcatca tctaccagga tgtgagcacc aacgtgggac
      661 ccatgaagca ggatcccgcc atggtgagct gttggccgaa cccggcgacg gacatcgtgc
      721 attgccgcat caacgggatc acgcccggct ccgccgtgaa cgttctggat ggccttggta
      781 ggatcgtggc cacaacgcgc acggacggtt caaatgatcc gagcatcacg atcgggcatc
      841 tggccgccgg cacctattcg ctgaggctcg cgagcggcgc ccaggtcgga tccttcatca
      901 aacgctgatc acagcaagca tcatgtgggt acatttcgtt cgggcgttcc gcaggtactg
      961 gccggacctg gcggtcagct tgatgcttgc tttcgttctt tacttgatct ggagaatgtg
     1021 aaccatggac aacacggtca gcaatttgca ctggtacatg catccccccc ggccattctt
     1081 gtccatggtt gtggggaggc gtccgtcagt ggccgtgaga tcgatccccc acgcgttgtg
     1141 ctttgcactg atcatgatcc ggccctctgc cgactccttc gcacaggcca cacccatcag
     1201 tgcgcacgaa gatgttcggc gctatgcgga ggtgatccgc caggaacatg tggatccgtt
     1261 cacctgcgtg agccgacagc acttcgatag ttgcgcagca gcgcttgatg agcggctttc
     1321 caacgaggac cggaccggcc tgctgatcgg ccttctgcgg ctcacggcaa tgctgaacga
     1381 tgagcacacc atggtgcggt ggaatgcccg ccttcggctg ccgatacagg tgcatccact
     1441 ggatgaggac ctgtgcatca tcggcacgga cagcgcgcat cggccattgc ttttgcatag
     1501 ggtgatcgcc atcaacgatg tgcccgcttc cagtgttctg gaacggttcg ccgcactggt
     1561 caagcaggac aatccttcct atgcggccgc atggctggga tggaacctgt gcgatgtgga
     1621 cctgctcatg ggactgggca ttgttaccga tccagaacat gtgcgactgg gcctgttgga
     1681 catggaggga gataccgtaa gcgcggaggt ccattccatt gatgcgcagg ctgtacagtg
     1741 ggtgcgacca gcgagcatgt gcctccaatg ggcatcacgc gtcaagggca actatggttc
     1801 gctcttcgat gacagcacca gcacattgta cgtgaactac aggcgttgcc gcgaggacga
     1861 gaacatttcc atgagcgcct tcaccgagga ggtgttcgcg cggatcgaag cgcaacaccc
     1921 tgctcgtctc gtcatcgacc tgcgcgagaa cgggggcgga agttcggcct tgttcatgcc
     1981 tttcatcaag cggctgcgca agggctatct gaacaaggaa aatcgcatcc gcgtgctcat
     2041 cggcaggggt acgttctctt cggcggtgat gaacgccgtg gaactgaagc gcaccacgtc
     2101 cgccgtactg atcggagagg tcaccggcgg taatgtgaac cactacggcg aagtgcgcga
     2161 ggaggctttg ccatggtccg gattcaaggt cctgtattcc accaaacatt gggtgaactg
     2221 gccgggtgtt gtgggaggct tgaaacccga tgaatatgtc gggccttcca ttgccgacgt
     2281 ggtgaacggt tcagatgcgg ccatcgaacg ggcgatcgat cgatgaacat gagcgcgcca
     2341 gtgacctcga tgcggatgtt gacatgcctc acgcgcaagc catgcgttct tgatcctggt
     2401 caagtgcggg gaggcggaac ggaagaactt tcgccgggtc tgcgacatcc gaccttgacc
     2461 cctgcttcct caatgaacac cacaccacat gggctggacc ggagcgagta tcctttcgca
     2521 gccatgcgta cgaacatggc gatggccgtg tacacaacat cgatgaaggc caaggccctg
     2581 tgatgctctt cgttcacggt acaccggact ggaaccttgg ttccaggcac ccgatcaaag
     2641 cgttcagcgc cacgcatcgc ggcgtggctg tggtccaccc gggtttctgg atcagtgata
     2701 aacctgccga tgccgactgc atggtggccg cgcataccag gcgccttcaa gggctgatcg
     2761 ataagctcga cctgaaggac ctgaccttgg tggtcaccga ctttggtggc ggcatcgggc
     2821 tgcaacatgc cttggagcgc ccctgtaatg tgaagcacgt ggtcctctac aacacctgga
     2881 tgtgggacct gatgcccgac aagcgcttcg cacggccggc tcgtatcatg aactcctggc
     2941 tgggccggtt cctcttatca gcgcatgggt tcactgtgaa catgatgatg cccaatgcct
     3001 acggtgacaa gtcaaggctc acgccgcgca tccacgcaca cgacaagaac gcattgccgg
     3061 acgcgtcttc gcgtgcagcc aacttcgctt gcgtacagga aatggcgaac gccggcccgt
     3121 tctggaacgc gcaatgggaa caggtcgatc gttcgcgcaa catgcgcgtg ctgagctgct
     3181 ggggcatgaa ggacgggttc ttaccgaacg acctgttgcc atggtggaag caggcctttc
     3241 ccgatgcggc ggtctcggct ttcccgcatc cgggcaagtc gtgcaagggg aagcgaacga
     3301 ggagttggtg gccgcgatgc ggacgttcct cgcctggatc gacca
//
LOCUS       DAOQOC010000189         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14068_length_3345_cov_3.524012, whole genome shotgun sequence.
ACCESSION   DAOQOC010000189 DAOQOC010000000
VERSION     DAOQOC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14068_length_3345_cov_3.524012"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PL106_04150"
     CDS             <1..286
                     /locus_tag="PL106_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32294.1"
                     /translation="DMSLVVELKRGDITHFYRATQLADVLRKGTSAVLIAAVKLSDIP
                     GHGEGLRLRTYLYNPGRKRALVSALSLRTRKGDPVLYGLFEPITGRLEFR"
     gene            complement(304..1128)
                     /locus_tag="PL106_04155"
     CDS             complement(304..1128)
                     /locus_tag="PL106_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNA32295.1"
                     /translation="MNEQVNKVNEWGEKALSSIIDYAPKILMALVILWIGTILIRMLA
                     RLLGKVLDRRGVEPTLKRFLHSLVTISLRILLFITCIQMLGVQTTSFIAIIGAAGLAV
                     GLALQGTLANFAGGTLILLFKPYKLGDLIDAQGVLGTVKEIQIFTTVLLTPENKTAII
                     PNGAMANGNIINYSADGRMRVDLAFMVNGSESVPRVREVLLRAMADDPKVLKDPAPSV
                     AVNKLHMEGVELAVRPWCDPAHYWDVYFGTYEKGREALAAAGIKGPQKEMVVKQGG"
     gene            1359..2786
                     /locus_tag="PL106_04160"
     CDS             1359..2786
                     /locus_tag="PL106_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNA32296.1"
                     /translation="MALSRFWTWMLLLSIGFVLFNLFSGRIYSLGILANGEQGEPLVV
                     AERDTLSVAGTEWLAALRSAGNAGVQQGDTLYTLSKAGNVQVHVGTVPADGLFPTCEN
                     TITGIWLPLIGYLTFFCGLLNLLVDARAMEKLARALAPIFHRIFPGLRKDHPAYGYMT
                     LNFAANFLGLDSAATPFGLKAMESMQADNPTKDRATDSQIMFLCLHAAGLTLLPASII
                     GYRAAKGAANPADIMIPLIITSFVGTLAAMVFVAIRQRINLLNLPVVLFVAGVGGLIA
                     GLMAYLMTLTGLGRSHFTDNLSNGLLLVIIALIVLYAFLKEKNFIEKGTNLFDSFIEG
                     AKDGFTTGLRVLPYMIAMLGALSIFRNSGLMGMVMDGMAWLLGAVGVSREVIDAIPVA
                     LMRPFSAGGARGFLLDAMETYGADSLTGRLVCLFQGAAETTFYVVALYFGSVNVKDSR
                     YALGVMLLVDLVCVITAVVVCAAYF"
     gene            2893..>3345
                     /locus_tag="PL106_04165"
     CDS             2893..>3345
                     /locus_tag="PL106_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNA32297.1"
                     /translation="MKQKVEVRGSRIHGRGVFATADIKKGEPIIEYTGRLITHAESDE
                     RYSTDMESGHTFLFILNDEWVIDGNREANDARWINTGCSPNAVAFVHEHKGKDRRKDK
                     VIIEARRAIAKGEEITYDYGIRLDTPPTKAELEAWTCRCGSPKCKGTML"
BASE COUNT          646 a         1005 c         1064 g          630 t
ORIGIN      
        1 cgacatgagc ctggtggtgg agttgaaacg gggtgacatc acccatttct accgcgccac
       61 gcaattggcg gatgtgctgc gaaaggggac cagtgcggtg ctcatcgccg cggtgaaact
      121 ttcggacatc cccggccatg gtgaggggct gcgcctgcgc acgtacctgt acaacccggg
      181 tcgtaaacgg gccctggtga gcgcgctgag cctgcggacg cgcaagggcg atccggtgct
      241 ttacggactg ttcgagccga tcacaggtcg gctggaattc aggtagagca ggcctcgcgg
      301 ccgtcagccg ccctgtttca ccaccatctc cttctggggc cctttgatcc ctgccgccgc
      361 aagtgcttcg cgtccctttt cataggtacc gaaatacacg tcccaataat gggccggatc
      421 gcaccagggc cgtacagcca gttcaacccc ctccatgtgg agcttgttca cggcgacgga
      481 aggtgcggga tccttcagga ccttcgggtc atcggccatt gcgcgcaaaa gcacctcgcg
      541 cacacgcgga acggactcgg aaccgttgac catgaaggcg agatcgacgc gcatacgacc
      601 atccgcgctg tagttgatga tgttcccatt ggccatggcg ccgttgggga tgatggcggt
      661 cttgttctcc ggcgtgagca gcacggtcgt gaagatctgg atctccttca ccgttccgag
      721 gacgccttgg gcatcgataa ggtcgcccag tttgtacggt ttgaagagca ggatgagcgt
      781 gcctccggcg aagttcgcca gtgtgccctg caaagccagg cccaccgcca ggcccgcggc
      841 accgatgatg gcgatgaaac tggtggtctg caccccgagc atctggatgc aggtgatgaa
      901 cagcagtatg cgcaaagaga tggtgaccaa gctgtgcagg aaccgtttca gcgtgggctc
      961 cacgccgcgc cgatcgagca ccttgcccag gagtcgggcc agcatgcgga tcaggatggt
     1021 accgatccac aggatgacga gtgccatcag gatcttgggg gcgtaatcga tgatggacga
     1081 cagggccttc tcaccccatt cattgacctt gttcacttgt tcgttcatgg gttcagcagg
     1141 ttggtgcggg aaactattgg acgacgcagg ccccggttcg tcacggggaa ggatggcgat
     1201 cttaccacag cgggacattc cggcatgcgt tcataccact ttcatgtacg cgcacggaca
     1261 caggccatcc acaccgggct gttggccgga acaagcggaa cgcccgtaca gcacaaccgc
     1321 cgctcacctc ctttgccacg atcagatcgc tgtagccgat ggccctgagc cgattctgga
     1381 cgtggatgct cctgctgagc atcgggttcg tgctgttcaa cctgttcagc ggccggatct
     1441 attcattggg catactggcc aacggggagc agggggaacc gctggtggtg gccgaacggg
     1501 acacgctgtc cgtcgcgggc accgaatggc tggcggccct gcgaagtgcc gggaacgcag
     1561 gtgtgcaaca gggcgacacg ttgtacaccc tgagcaaggc gggcaacgtg caagtgcatg
     1621 tgggcacggt accggccgac ggcctgttcc ccacctgcga gaacacgatc acgggcatct
     1681 ggttaccgct catcgggtat ctcaccttct tctgcgggct gttgaacctg ctggtggacg
     1741 cgcgggccat ggagaaactg gcgcgcgcgc tcgcccccat ctttcaccgg atcttcccgg
     1801 gtctgcgcaa ggaccacccg gcgtacgggt acatgaccct gaacttcgcg gcgaacttcc
     1861 tcgggctgga cagcgcggcc accccattcg gcctgaaggc catggagagc atgcaggccg
     1921 acaatcccac gaaggaccgc gccacggaca gccagatcat gttcttgtgc ctgcacgctg
     1981 cgggcctcac actgcttccg gcgagcatca tcggctaccg ggcggccaaa ggcgcggcga
     2041 atccggcgga catcatgatc ccgctgatca tcacctcgtt cgtgggcacg ttggcggcca
     2101 tggtgttcgt ggccatccgc cagcgcatca acctgctgaa cctgcctgtg gtcctcttcg
     2161 tggcgggcgt cggaggcctc atcgcggggt tgatggccta cctgatgacc ctgaccggcc
     2221 tcggcagatc gcacttcacg gacaacttga gcaatggact gctgctggtc atcatcgccc
     2281 tgatcgtgct gtacgccttc ttgaaggaga aaaacttcat cgagaaggga acgaatctgt
     2341 tcgactcctt catcgagggc gccaaggacg gcttcaccac gggccttcgc gtgctgccgt
     2401 acatgatcgc catgctcggt gcgctcagca tcttccgcaa cagcgggctc atgggcatgg
     2461 tgatggacgg catggcgtgg ctgcttggcg cggtgggggt gagccgcgaa gtgatcgatg
     2521 cgatacccgt ggcactcatg cgtccgttca gcgccggagg tgcgcgcggt ttcctgctcg
     2581 acgccatgga aacgtacggg gccgacagcc tcaccggccg cctggtctgt cttttccagg
     2641 gtgcggcaga aaccaccttt tacgtggtag cgttgtattt cggcagcgtg aacgtgaagg
     2701 acagccgcta cgcgctcggg gtgatgctgc tggtggacct ggtgtgcgtg atcacggctg
     2761 tggtggtgtg cgcggcctat ttctgatgtg ctggaggaac tcgcaggacc atgtcgggcg
     2821 catgccttgt tgatgcaaat tcgcggccgc aaagccgacc ggccccagcg acgaacgatc
     2881 attccggtag acatgaaaca gaaagtggaa gtccgcggat cgaggatcca tggccgtggc
     2941 gtgttcgcca ccgccgacat caagaaaggt gagcccatca tcgagtacac gggcaggctg
     3001 atcacgcacg cggaatcgga cgaacgctac agcacggaca tggaatcggg ccacacgttc
     3061 ctgttcatcc tgaacgatga atgggtgatc gacggcaacc gcgaagccaa cgatgcacgc
     3121 tggatcaaca ccggttgctc gcccaacgcg gttgccttcg tgcatgagca caagggcaag
     3181 gaccgccgca aggacaaggt gatcatcgag gcccgccggg cgatcgccaa gggcgaggag
     3241 atcacgtacg attatgggat cagactggac acaccgccca ccaaggccga attggaagcc
     3301 tggacgtgcc gttgcggatc gcccaagtgc aaggggacca tgctc
//
LOCUS       DAOQOC010000190         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14123_length_3338_cov_2.983856, whole genome shotgun sequence.
ACCESSION   DAOQOC010000190 DAOQOC010000000
VERSION     DAOQOC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14123_length_3338_cov_2.983856"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PL106_04170"
     CDS             complement(<1..639)
                     /locus_tag="PL106_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA32298.1"
                     /translation="MKTETRLLHALPHDPLTGAVSVPIYQTTTFEQEGPGINKGFDYS
                     RTNNPTRQAFEQLLAELEGGTKGIAFSSGLAAVDAVLKLLSAGDEVIAVDDIYGGTYR
                     ALHTIYNRLGITVKHVDTSDPGNVLEAITPRTKLVWLETPTNPTLKVSDIRAIASISK
                     ASGALLLVDNTFGSPVAQQPLKLGADIVLHSITKYIGGHSDVIGGGIVVKDEE"
     regulatory      complement(822..926)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1056..1709)
                     /locus_tag="PL106_04175"
     CDS             complement(1056..1709)
                     /locus_tag="PL106_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HNA32299.1"
                     /translation="MGKRIIITGATGYVGEGVLLECLAHPAIDAVLVIGRKSCARQHA
                     KLKELLVKDFFSLDNEAPHLRGYDGCFFCAGISSVGMKEEEYTRITHDATLAFAQAVH
                     AASPDAVFTYVSGAGTDSSGKGRVMWARVKGRTENDLARIGFKANYNFRPGIMTITPG
                     MRNPKRWMRMLVPVFAVLMPWTTSSMKQVGLAMINCVLKGYPKPVLGVKDIKALAAA"
     gene            complement(1728..2837)
                     /locus_tag="PL106_04180"
     CDS             complement(1728..2837)
                     /locus_tag="PL106_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32300.1"
                     /translation="MRVLPVLLKKEFLQIFRNKAILIISLVAPIMQFLVLPLAASFEI
                     KNIDLVVVDQDRSDLSRQLLERITASGYFRLVDYAPSYASAYALVEADAADLVLQVPS
                     GFERNLMREHVQQVFIAVNAINGMKAGVGGAYLGTILRSFNSSVVATYAPGSAPEGIA
                     VTSSGLFNPERNHRIAFIPGILAILVTMIGGMLSALNIVKERETGTIEQINVTPIRKR
                     DFILGKLIPFWIMAMVVFTIGLLLAWFVYGIAPQGSVWLLYAFVAVYLVAILGFGLLV
                     STYCASQQQAMFVMFFFVMIFILLGGLFTPVDSMPAWAQVMTRFNPVAHMIEVMRLVM
                     LKGSGFMAILPNFLAIAAMAVVLNTWAVMNYRKTG"
     gene            complement(2841..3044)
                     /locus_tag="PL106_04185"
     CDS             complement(2841..3044)
                     /locus_tag="PL106_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32301.1"
                     /translation="MFPIEDMPVALQVLANVVPSKWYYTIVKDVMIKGAGFSAIWRET
                     AVLLGMTLFLFVLCLRKFKIRLA"
     gene            3043..>3338
                     /locus_tag="PL106_04190"
     CDS             3043..>3338
                     /locus_tag="PL106_04190"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNA32302.1"
                     /translation="MKPLRMTVGSNMPINEANIADTCGKCHYGVEETYLKSVHGQLLK
                     KGDKAGPVCTDCHTAHEVEQPRTGHFKMASDQRCGKCHQDSLLHYRDTYHGKAMA"
BASE COUNT          655 a         1018 c         1057 g          608 t
ORIGIN      
        1 ctcctcgtcc ttcaccacga ttcccccgcc gatcacgtcg ctgtggccgc cgatgtactt
       61 ggtgatgctg tggagcacga tgtccgcgcc gagcttcagc ggttgctgcg ccacgggcga
      121 gccgaaggtg ttgtccacca gcagcagcgc gccggaagcc ttggagatgg aggcgatggc
      181 gcggatgtcg ctcaccttga gcgtggggtt ggtgggcgtt tccagccaca ccaatttcgt
      241 gcgcggggtg atcgcctcga gcacgttgcc gggatcgctg gtgtccacgt gcttcacggt
      301 gatgccaagc cggttgtaga tggtgtgcag cgcgcggtag gtgccaccgt agatgtcgtc
      361 cacggcgatg acctcgtcgc ccgcgctgag cagcttcagc accgcgtcca cggcggccag
      421 cccgctgctg aaggcgatgc ccttggtgcc gccttccagc tcggcgagca gttgctcgaa
      481 ggcttggcgc gtggggttgt tggtacggct gtagtcgaag cccttgttga tgccgggccc
      541 ttcctgctcg aaggtggtgg tctggtagat gggcacgctc acggcgccgg tgagggggtc
      601 gtggggcaag gcgtgaagaa ggcgggtctc ggttttcatg gcttttctct ggttgtttgt
      661 cggggcgcga catgtcgcgc ccgtacgggt tttaggtgtt ggggacacca ccgcaccgga
      721 aaagcctccg tcagcgaccg ccgcacttgc ctaccggcag gtaggaaaag cgaacggtcc
      781 ctgccggata agccgatagg gaccgttgaa ggtctttgat cgtcttatct ctccccgttg
      841 ccggggcagg agttggcacc gctgccctta cggaccggtt gccaaggttt cacagggcct
      901 gaaccctcca cctttctgga taagtgctgt ctggaaaaga acgcggcgca aatctaatgg
      961 tcaagggcgt gatctttcca aatgcgggga tcactttctt cggaaaggcg cgtccggcaa
     1021 gggcccggcg gggtacgtca acggcggaac tcccttcacg ctgcggccaa cgccttgatg
     1081 tccttcaccc cgagcaccgg cttggggtat cccttcagca cacagttgat catcgccaag
     1141 cccacctgct tcatcgaact ggtggtccag ggcatcagca cggcgaacac cggcaccaac
     1201 atgcgcatcc accgcttggg gttgcgcatg ccgggcgtga tcgtcatgat ccccggcctg
     1261 aaattgtagt ttgccttgaa gccgatgcgc gccaggtcgt tctcggtgcg gcccttcacg
     1321 cgcgcccaca tcacgcggcc cttcccactg ctgtcggtgc cagcaccgga gacgtacgtg
     1381 aagacggcat ccggtgaagc ggcatgcacc gcttgcgcga aagcaagcgt cgcgtcatgg
     1441 gtgatgcgcg tgtattcttc ttccttcatg cccacggaac tgatgcccgc gcaaaagaag
     1501 cagccgtcgt agccacggag gtgcggcgct tcgttgtcca gtgagaagaa atccttcacg
     1561 aggagctcct tcagcttggc atgctggcgg gcacagctct tccgaccgat caccaggaca
     1621 gcatcaatcg ctggatgtgc caggcattcg agcagaacgc cttcgcccac ataacccgtg
     1681 gcccccgtga tgatgatgcg cttgcccata ccgcaaagga aaggtgctca acccgtcttc
     1741 cggtagttca tcaccgccca cgtgttcagc accaccgcca tggccgcgat ggccaggaag
     1801 ttgggcagga tggccatgaa gccgctgcct ttcagcatca ccaagcgcat cacctcgatc
     1861 atgtgcgcca ccgggttgaa gcgcgtcatc acctgggccc aagccggcat gctgtccacc
     1921 ggggtgaaga gccctccgag caggatgaag atcatcacga agaagaacat cacgaacatg
     1981 gcctgctgct ggctggcgca ataggtggag accagcaaac cgaagccgag gatggcgacc
     2041 aggtacaccg cgacgaaggc gtacaacagc cacacggagc cctgcggagc aatgccgtag
     2101 acgaaccagg cgagcaggag cccgatggtg aagaccacca tggccatgat ccagaaaggg
     2161 atcaatttgc ccaggatgaa atcgcgcttg cggatgggcg tgacgttgat ctgctcgatg
     2221 gtgcccgtct cccgttcctt cacgatgttc agggcgctga gcatgccgcc gatcatggtg
     2281 acgaggatgg caaggatgcc ggggatgaag gcgatccggt ggttccgttc cgggttgaac
     2341 aggccggagg aggtgacggc gatcccttcg ggtgccgagc ccggcgcgta cgtcgccacc
     2401 acggagctgt tgaaggacct caggatggtg ccgaggtagg cgccgcccac gccggccttc
     2461 atcccgttga tcgcgttcac ggcgatgaac acctgctgca catgctcccg catcaggttg
     2521 cgctcgaagc cggaagggac ctgcaggacc aggtcggcgg cgtcggcctc caccaaggca
     2581 taggcactgg cgtaggatgg cgcatagtcc accagccgga aatagcccga ggcggtgatg
     2641 cgctcgagca gttgccgcga caggtcggag cggtcctggt ccaccaccac caggtcgatg
     2701 ttcttgatct cgaagctcgc cgccagcggc agcacgagga actgcatgat gggcgccacc
     2761 agcgaaatga tcaggatggc cttgttcctg aagatctgca ggaactcctt cttgagcagg
     2821 acggggagta cgcgcatggt tcaggccagt ctgatcttga atttccgcag gcacagcacg
     2881 aagaggaaca gcgtcatccc cagcagcacc gccgtttccc gccatatcgc cgaaaagccg
     2941 gcgcccttga tcatgacgtc cttcacgatg gtgtaatacc actttgacgg tacgacgttg
     3001 gcgagcacct gcaacgccac gggcatgtcc tcgatgggga acatgaagcc gctgaggatg
     3061 accgtgggca gcaacatgcc gatcaacgag gcgaacatcg cggacacctg cggcaaatgc
     3121 cactacggcg tggaggaaac ctatctcaaa agcgtccacg gccaattgct gaagaagggc
     3181 gacaaggccg ggcccgtctg cacggattgc cacacggcac acgaggtgga gcagccccgg
     3241 accggccact tcaaaatggc cagcgaccag cgctgtggaa agtgccacca ggacagcctc
     3301 ctgcattacc gcgacaccta tcacgggaag gctatggc
//
LOCUS       DAOQOC010000191         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14129_length_3337_cov_3.706277, whole genome shotgun sequence.
ACCESSION   DAOQOC010000191 DAOQOC010000000
VERSION     DAOQOC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14129_length_3337_cov_3.706277"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>3337
                     /gene="ileS"
                     /locus_tag="PL106_04195"
     CDS             8..>3337
                     /gene="ileS"
                     /locus_tag="PL106_04195"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNA32303.1"
                     /translation="MASFYDRIVPKVAKEVLQQWEAEDTFGQSLELRKDAPPFVFYEG
                     PPSANGLPGIHHVMARTIKDIFCRYQTQKGHRVDRKAGWDTHGLPIELGVEKELGITK
                     EDIGKKISIEEYNAACKKAVMRYTDVWNDLTKRIGFWLDLEHPYVTYHTKYIESVWYL
                     LKKLYDDDLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKPVKDTSAVALFKVIRDQNS
                     EWLFSPSPREKGPGDEGVFIMAWTTTPWTLPSNTALTVGPKLEYVRVKTFNPYTHAPQ
                     TVVLAKARLNAYFKEETKDASFDDYKKDGKNIPWTIVGEFLGHQLEGVRYEQLLPYAE
                     PEGGGDAFKVIGGDFVTTEDGTGVVHTAPSFGADDQRVARQHNIGSLTLVDLRGRFKP
                     EVTDFAGEYVKAEYYTAEELAAEAKKQGRDKYLSVDERIAIKLKTEGRAFKVEKYEHN
                     YPHCWRTDKPVLYYPLDSWFVRVTAKKDRLIALNKTITWKPESTGTGRFGNWLENLQD
                     WNLSRSRYWGIPLPIWRTEDGGEELCIGSLKQLKEEIARAVKAGVMKEDPYASFQVDD
                     LSDANYDRVDIHRPFVDHIVLVSPSGKPMKRETDLIDVWFDSGAMPYAQWHYPFENEA
                     TFKEKFPAAFIAEGVDQTRGWFYTLHAISTLVFDQVAYKTVVSNGLVLDKVGQKMSKR
                     LGNAVDPFTTLDQYGADAVRWYMISNASPWDNLKFDAEGITEVQRKFFRALFNTYNFF
                     ALYANIDGYDGKGEATLTESDRWVLSRLNSVMKVADTNYGDYEPTIAARTIQDFVVED
                     LSNWYVRLNRRRFWKGDALGSARGGDKNAAYQTLHRCLEVVAMLSAPIAPFFSDRLYR
                     DLHAGTPSGTEGVHLSDWPKHDERAIDLELEQRTSLAQKLTSLVLSIRKKEGHRVRQP
                     LQKMMVPVTDAAMRTRLDAIRDLVLSEVNVKELVILDASESKLTKKIKPDFKKLGARM
                     GKLMKSVANAVNAFDQAQIAELEAKGSMKLMVDGQEVELLREDVEITAENVPGLSVAS
                     DGALTVALDITLTDALIQEGIARELVSRIQTLRKESGLEVTDRIELRIQRNGDPAFER
                     AVKAHAPHILAETLALTAVDQL"
BASE COUNT          749 a         1022 c         1031 g          535 t
ORIGIN      
        1 caccggcatg gcatccttct acgaccgcat cgtgcccaag gtcgccaagg aggtcctgca
       61 gcaatgggag gccgaggaca ccttcggcca aagcctggaa ctgcgcaagg acgcgccccc
      121 tttcgtgttc tatgagggcc cccccagcgc caacgggctg cccggcatcc accatgtgat
      181 ggcccgcacc atcaaggaca tcttctgccg ctaccaaacg cagaagggcc accgcgtgga
      241 ccgcaaggcc ggctgggaca cgcacggcct gcccatcgaa ctgggcgtgg agaaggagct
      301 cggcatcacc aaggaggaca taggaaaaaa gatctccatc gaggagtaca acgcggcgtg
      361 caaaaaggcc gtgatgcgct acaccgacgt gtggaacgac ctcaccaagc gcatcggttt
      421 ctggctcgac ctggagcacc cgtacgtcac ctaccacacc aagtacatcg agagcgtgtg
      481 gtacctgctg aagaagctct atgacgacga cctgctgtat aaaggctaca ccatccaacc
      541 gtattcgcct gcggcgggca cgggcctttc atcacacgag ctcaaccaac ccggcactta
      601 caagccggtg aaggacacca gcgcggtggc gctcttcaag gtgatccgcg atcagaacag
      661 cgaatggctt ttctcccctt ctccccggga gaaggggccg ggggatgagg gcgtcttcat
      721 catggcttgg accaccacgc catggaccct gcccagcaac accgcgctca ccgtgggccc
      781 caagctggag tacgtgcgcg tgaagacctt caacccgtac acccacgcgc cgcagaccgt
      841 ggtgctggcg aaggcacgat tgaatgccta tttcaaggaa gagaccaagg acgcttcgtt
      901 cgacgactac aagaaggacg ggaaaaacat accgtggacg atcgtcggcg aattcctcgg
      961 ccaccaattg gaaggtgtac gttacgagca actgttgccc tacgccgaac ccgagggcgg
     1021 tggcgatgcc ttcaaggtga tcggtggtga tttcgtcacg accgaggacg gtaccggcgt
     1081 ggtacacacg gcgccaagct tcggtgcgga tgaccaacgc gtggcgcgtc agcacaacat
     1141 cggttcgctc acattggtgg acctgcgcgg ccggttcaaa ccggaggtga ccgacttcgc
     1201 tggcgaatac gtgaaggccg agtactacac ggcggaggag cttgccgccg aagcgaagaa
     1261 gcagggccgc gacaagtacc tcagtgtgga cgagcgcatc gccatcaagc tgaagaccga
     1321 gggccgcgcc ttcaaggtgg agaagtacga gcacaactac ccgcactgct ggcgcaccga
     1381 caagcccgtg ctctactacc cgctggacag ctggttcgtg cgtgtgaccg cgaagaagga
     1441 ccggttgatc gcgctcaaca agaccatcac ctggaaaccc gagagcaccg gcaccggccg
     1501 cttcggcaac tggctggaga acctgcagga ctggaacctc tcgcgcagcc gctactgggg
     1561 catcccgctc ccgatctggc gcacggagga cggtggtgag gaactgtgca tcggctcgtt
     1621 gaagcagttg aaggaagaga tcgcgcgtgc agtgaaggcg ggcgtgatga aggaggatcc
     1681 ctacgcatcg ttccaggtgg acgacctgag cgatgccaac tacgaccgcg tggacatcca
     1741 ccgcccattc gtggaccaca tcgtgctcgt ctccccaagc ggcaagccga tgaagcgaga
     1801 gaccgacctg atcgatgtgt ggttcgacag tggtgcgatg ccctacgcgc aatggcacta
     1861 ccccttcgag aacgaggcca ccttcaagga gaagttcccg gccgccttca ttgcggaagg
     1921 cgtggatcaa acgcgcggct ggttctatac cctgcacgcc atcagcacct tggtctttga
     1981 tcaggtggcc tacaagaccg tggtgagcaa cggcctggtg ctggacaagg tgggccagaa
     2041 gatgagcaaa cgcctaggca acgccgtgga tccgttcacc accctggacc agtacggtgc
     2101 cgatgccgtg cgctggtaca tgatcagcaa cgcgtcgccg tgggacaacc tgaagttcga
     2161 cgccgaaggc atcaccgaag tgcagcgcaa gttcttccgt gcgctcttca acacctacaa
     2221 cttcttcgca ctgtacgcca acatcgatgg gtacgatggt aagggcgaag ccacgctcac
     2281 ggaaagcgat cgctgggtcc tctcgcgcct caacagtgtg atgaaggtgg ccgatacgaa
     2341 ctacggcgac tacgagccca ccatcgccgc gcgcaccatc caggacttcg tggtggagga
     2401 cctgagcaac tggtacgtgc gcctcaaccg ccgccggttc tggaaaggcg acgccctcgg
     2461 ctccgctcgg ggaggcgaca agaacgcggc ctaccaaacc ttgcatcgtt gcctggaggt
     2521 ggtcgccatg ctcagcgcac cgatcgcgcc tttcttcagc gatcggttgt accgcgacct
     2581 tcatgctgga accccgagcg gaaccgaggg ggttcacctg agcgactggc cgaagcacga
     2641 tgaacgtgcc atcgacctgg agctggagca acgcacctcg cttgcccaaa agctcacctc
     2701 cctggtgctg agcatccgca aaaaagaagg ccatcgggtg cgccaaccct tgcagaagat
     2761 gatggtgccc gtgacggacg ccgccatgcg cacacgcttg gatgcgatcc gcgacctcgt
     2821 gttgagcgaa gtgaacgtga aggagctggt gatcctggat gccagcgaga gcaagctcac
     2881 caagaagatc aaacccgact tcaagaagct cggtgcgcgc atgggcaagc tgatgaagag
     2941 cgtggctaat gccgtgaatg ccttcgatca ggcgcagatc gcggaattgg aagcgaaggg
     3001 cagcatgaag ctcatggtgg atggccagga ggtggaactg ctgcgcgagg atgtggagat
     3061 caccgccgag aacgtgcccg gcctgagcgt ggccagcgat ggggccctca ccgtggcgct
     3121 ggacatcaca ctcaccgacg ccttgatcca ggagggcatc gcccgtgaac tggtgagccg
     3181 gatccagacc ctgaggaagg agagcgggct ggaggtaacg gaccggatcg agctgaggat
     3241 ccagcgcaat ggcgatcctg ccttcgaacg ggccgtaaaa gcccacgcac cccacattct
     3301 ggccgaaaca ttggcgctga ccgcggtgga ccagctg
//
LOCUS       DAOQOC010000192         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14280_length_3317_cov_3.580932, whole genome shotgun sequence.
ACCESSION   DAOQOC010000192 DAOQOC010000000
VERSION     DAOQOC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14280_length_3317_cov_3.580932"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PL106_04200"
     CDS             complement(<1..385)
                     /locus_tag="PL106_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA32304.1"
                     /translation="MEPELTAVLVDDEEHCTDMLAWMLAEYCPQVHVAAIFNNPEKAL
                     HHLRTNRPDVLFLDIEMPGLNGFDLLTRLGHQPRALVFCTAYDQFAIKAIKHQALDYL
                     LKPVDKDELRTAVSKAAVQNEDPDLL"
     gene            complement(370..>3317)
                     /locus_tag="PL106_04205"
     CDS             complement(370..>3317)
                     /locus_tag="PL106_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA32305.1"
                     /translation="RFRRFDLDRPVKGGAPRDIAEGLAQAPDGTIWIASMHAGLLRFD
                     PATEHWVSWTEMTGDTLADEFMIGPAICVLSNDSILVGAVNRGIFLYLVRERRMQRVV
                     APLPVRLFHTSHILRDRSHLRRFWISASGAFYAYDLGSDSLQRVRLNDSDDPDQGISV
                     NFLAQRSDGAVWLSTWGDGLIRYDPGTDSSRCWLFDTIPPLTGAKNVEFAMAAMNEDE
                     FWVGTHRGLRRFSQQNGFGPLIDHDPEDANSLTGGWVKGVLLDQEGNLWCATQSGLSC
                     MAPGQNRFHSFTFGKAYYRGDQQPGIQATLELGDRFLVATDDLGLFLIDREHGREEHL
                     QLHDAKGKKITDPHVIDMFRCGEGPIELRTGAGAFLLDPESWRIRPVLQRTIARFGQL
                     DMLGSLCDTAGWVWTGAPFGLYHTNSDKDTGYAYDRNAPPGYRVDRARWAQGIARDRS
                     GHTWFSVNETGIGRVPPKGSPVEVMTPAGCAWLPTPNVWDLTVDPNNRLWLGTRGYGL
                     VTTPADDPGGPGTRSLSTSEGMGTSIGTVMTDHAGDIWAAGPSGLYRVNGDDMRVKRF
                     RTDDGLRSAAFLHDKITQHASGLITIEHHNTLLWFHPDSLDEPLTAPRVWIDGASVNG
                     LPVTRDSTLNAAPHLRLPHDSNYVDIHFSAIALTQRHRITLEYRLIGNSERWVSTAAS
                     GSAIFTALPPGDYTFEVRAADMAEAPVTRLALTIIPAFWQTAWFKALIGLLVIAELMI
                     AVRWRLRNVRREERLRSDFERRIGEVEMSALRAQMNPHFLFNSLNSINRYIVKHEPRI
                     ASEYLTKFSRLMRSVLNNSKEKLVPLKDELEALTLYIDLEALRFTNKFDLRSQVEVDR
                     DPALIMVPPLMLQPFVENAIWHGLMQKKEGEAVLSLHVSHQQGVLRCTIEDNGIGRAQ
                     AAAIRSLSAIQRPSYGTRITQERLDLLKRLYGMEVQVVYTDLMDAAQRPLGTRVTLTI
                     QQQEPWNPN"
BASE COUNT          614 a         1014 c         1020 g          669 t
ORIGIN      
        1 caagcaggtc cgggtcctcg ttctgtacag cggccttgct caccgcggtc cgcagttcgt
       61 ccttgtccac cggcttcagc aggtagtcca acgcctgatg cttgatggcc ttgatggcga
      121 attgatcata ggccgtgcag aacaccagcg cacgcggttg atgaccgaga cgtgtcaata
      181 ggtcgaagcc gttcagcccg ggcatctcga tatccaggaa cagcacatcc ggccggttgg
      241 tgcgcagatg atgaagtgcc ttttccgggt tgttgaagat ggcggccacg tgtacttgcg
      301 gacagtattc cgcgagcatc caggccagca tgtccgtgca gtgttcctcg tcatcaacga
      361 gtacggcggt cagttcgggt tccatggttc ctgctgttgg atggtaaggg tgacccgcgt
      421 acccagcggg cgttgggccg cgtccatcag atcggtgtat accacttgca cttccatgcc
      481 gtaaaggcgc ttcaacaggt ccaatcgctc ctgcgtgatg cgtgtgccgt aggacggtcg
      541 ttggatcgcg gataggctcc gtattgccgc ggcttgggcg cggccgatcc cgttatcctc
      601 gatggtgcag cgcagcacgc cctgttggtg gctgacgtgg agcgagagca ccgcctcccc
      661 ttcctttttc tgcatcaatc cgtgccagat ggcgttctct acgaaaggct gcagcatcaa
      721 agggggcacc atgatgagcg cggggtcccg gtccacttcc acctgcgagc gcagatcgaa
      781 tttgttagtg aagcgcagcg cctccagatc gatgtagagg gtgagcgcct ccagttcgtc
      841 cttcagcggg accagcttct ccttgctgtt gttgagcacc gagcgcatga gccgcgagaa
      901 cttggtgagg tattccgacg cgatccgcgg ctcgtgcttc acgatgtatc gattgatgga
      961 gttgaggctg ttgaagagga agtgcgggtt catctgggca cgcagggcgc tcatctccac
     1021 ctcgccgatg cgccgttcga aatcggaacg gagccgctcc tcccggcgta cgttgcgcag
     1081 gcgccaacgc accgcgatca tgagctcggc aatgaccaac aggccgatca acgctttgaa
     1141 ccaggcggtc tgccagaaag cgggaatgat ggtgagggcc agacgggtta cgggagcttc
     1201 cgccatgtcg gctgcacgca cctcgaaggt gtagtctccg ggaggcagcg cggtgaagat
     1261 ggcggagccg ctggccgcgg tactcaccca gcgttcgctg ttgccgataa gccggtattc
     1321 cagggtgatg cggtggcgct gcgtgagggc gatggccgag aagtggatat ccacgtagtt
     1381 gctgtcatgt ggaaggcgca gatgaggtgc ggcattcagg gtgctgtcgc gcgttacggg
     1441 gaggccgttc accgaggcac catcgatcca tacgcggggt gcggtaagcg gttcatcgag
     1501 gctgtccggg tggaaccaca acagtgtgtt gtggtgctcg atggtgatga gaccggacgc
     1561 atgctgcgtg atcttgtcgt ggaggaatgc cgccgaacgc agcccgtcat ccgtgcgaaa
     1621 gcgctttacg cgcatgtcgt ccccattcac ccgataaagt ccggaagggc ccgccgccca
     1681 gatatcgccc gcgtgatcgg tcatgaccgt tccgatgctt gttcccatgc cctcggatgt
     1741 gctcagcgac cgcgttcccg gacctccagg atcgtcggcc ggagtggtga ccagtccgta
     1801 accacgtgtt cccagccaca accggttgtt gggatcaacc gtcagatccc acacgttggg
     1861 ggttgggagc cacgcgcaac ctgcaggggt catgacttca accggagaac ccttcggggg
     1921 aacgcggccg atccctgttt cgttcacgct gaaccaggtg tggccggatc gatcgcgtgc
     1981 gatcccctgt gcccatcgcg cacggtccac acgataccct ggcggggcgt tccggtcata
     2041 ggcatatccg gtatccttat cgctgttggt gtggtagagt ccgaagggtg cccctgtcca
     2101 gacccaaccg gccgtatcgc acaagctgcc aagcatatcc aactggccga accgggcgat
     2161 ggtgcgctgt aggacgggcc ggatgcgcca actctcaggg tccagcaaaa aggcccccgc
     2221 tcctgttcgg agttctatcg gcccttcacc acaccggaac atgtcgatga catgcgggtc
     2281 agtgatcttc ttccccttcg cgtcgtgcaa ctgcaggtgt tcctcccggc cgtgttcgcg
     2341 gtcgatcaag aacaacccta ggtcatccgt ggccacgagg aaacgatcgc ccagttccaa
     2401 cgtggcctgt atccccggtt gttgatcgcc tcggtagtac gccttcccga acgtgaatga
     2461 atggaaacgg ttctgcccag gtgccatgca cgagagtccg ctctgcgtag cgcaccacaa
     2521 attgccctcc tggtccagca gcaccccttt cacccaccca cccgtcaggc tattggcatc
     2581 ctccggatca tgatcgataa gtggaccgaa tccgttctgt tggctgaacc gtcgcaggcc
     2641 ccggtgcgtg cccacccaga attcatcctc gttcatggcc gccatcgcga actccacgtt
     2701 cttcgctccg gtgagcggag ggatcgtatc gaacagccag cagcgggaag aatccgttcc
     2761 cggatcgtag cgaataagcc catcgcccca ggtcgacaac cacacggcgc cgtcgctccg
     2821 ttgggccaag aagttcacgc tgatcccctg atccggatcg tcggaatcgt tcagcctcac
     2881 ccgctgaagt gaatcacttc cgaggtcgta agcatagaac gcaccggagg cgcttatcca
     2941 gaatctgcgc aggtgcgatc tgtcccgcag gatgtggctg gtgtggaaga gcctcaccgg
     3001 caacggtgcg actacgcgct gcatgcggcg ctctctcacc aggtaaagga agatcccccg
     3061 gtttacggcg cccaccagaa tggagtcgtt cgacagcaca cagatggcgg gtccgatcat
     3121 gaactcatcg gcgagggtat cgccggtcat ctcggtccaa ctcacccaat gttccgtcgc
     3181 gggatcgaac cgcaggagcc cggcgtgcat actggcgatc cagatggtgc catccggggc
     3241 ttgggccaag ccttcggcga tatcgcgcgg ggctccgccc ttcaccggcc gatccaggtc
     3301 gaacctccgg aagcgct
//
LOCUS       DAOQOC010000193         3311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14325_length_3311_cov_3.919533, whole genome shotgun sequence.
ACCESSION   DAOQOC010000193 DAOQOC010000000
VERSION     DAOQOC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14325_length_3311_cov_3.919533"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2371
                     /locus_tag="PL106_04210"
     CDS             <1..2371
                     /locus_tag="PL106_04210"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNA32306.1"
                     /translation="ERSPSLLTPMHHHSALSIRFLAIAALLALNVSAQNLPDEYHVSD
                     DGLRLVAGDLPVDGLYSVDGIKVIHLTFNQPDFWTLLDQSHQTGVHILASLEYDGQVF
                     DSVGVRIKGQTSYMMLPPGSQKFSFDINMEEFVDGQDLGGYETVNLNNGFQDPSVMRD
                     VIYHHLLRPHVPAARACFAQLFINGQNWGHYSLVQDLDSDFIREWWWSNDGSRWRADR
                     PDGDVFGGQWGDGTAGLNYLGPDTADYQEYYTLGRSEQENPWDALVTVCDKLNNTPIA
                     QLNDTLDKYMDIDRVLWHLAGENAFGDDDSYIHKGKMDYSLYWDPETGRMTTQELDGN
                     SILGTESLNWSPFYHANNVNYPLLNRVLQVPELRQRYLAHLRHIIATSMQQADVAALI
                     AQYRSLIDTVVQDDPIKLMTWSQYQSSCTTLGNRFNTRRNALNNNTEVAQQAPTIGNV
                     EHETGAGIGHPPVANETVHVRATVSSTNGINRVGLYWSNVLPGRFSRMDMHDDGAHND
                     GSAGDGVFGADIPGQPGGFWVRYYVAAEADNTAHSTAFSPPGAEHDVWIYQVTPTVSP
                     TEGVVINELMASNSSTVMDNNDQYEDWVELYNHGSAPVDLEGWFLTDTPFEPTKWEFP
                     GGTVLAPGMYMIVWPDEDQSQGLMHANFKLSASGESVLLYDPDTLLADQVDFGQQITD
                     MGYARVPNGTGPFVIQAPTFAASNDVTGFDEVAPNAGFRMYPNPASTHVILVWDGAGT
                     HTVTIHDATGRLVRNLILNSGSILETSTFGPGTYTVRCAQATQRLVVVH"
     gene            complement(2465..3217)
                     /locus_tag="PL106_04215"
     CDS             complement(2465..3217)
                     /locus_tag="PL106_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA32307.1"
                     /translation="MEHVPFVLSGGGARGIAHLGVLQAFHEAGIGPSAISATSAGSLI
                     GAFIAAGLTPMEASALLHQEWQLHRTRWKLLRGELLTQRRIGEFLEKYLPVKYFEDLR
                     VPLYVSATDLEHGGQRIFSAGEIIPPVLAACAVPLIFPPVVIDGTPYVDGGLSNNLPV
                     EPFEGRRSEVIAVYVNPLPPYSAQRTVRRTLDRTFHLSFREMVTRSAKGCRMFIEPPG
                     LAEYGMFDLSHSARIQRIGYDHTRALLADRHA"
BASE COUNT          691 a         1062 c          997 g          561 t
ORIGIN      
        1 cgaacgatca ccctcgctcc tcactcccat gcatcatcac tccgcacttt ccatccggtt
       61 ccttgccatt gccgcgcttc tggcattgaa cgtatcggcc cagaacctgc ccgatgagta
      121 ccacgtcagc gatgacggtc ttcggttggt cgcgggtgac ctgccggtgg atgggctcta
      181 ctccgtggat gggatcaagg tgatccacct caccttcaac cagcccgact tctggacgtt
      241 gctcgaccag agccaccaga caggcgtgca tatcctggcc tccttggaat acgacggcca
      301 ggtgttcgac tccgtcggtg tccgcatcaa gggacagacc agctacatga tgctgccgcc
      361 cggctcacag aagttcagct tcgacatcaa catggaagaa ttcgtggacg ggcaggacct
      421 gggcggatac gagacggtca acttgaacaa cggcttccag gacccgagcg tgatgcgtga
      481 cgtgatctac caccacctcc tccgcccgca cgtgccggcc gcgcgggcct gcttcgccca
      541 gttgttcatc aacggccaga actggggcca ctacagcctg gtacaggacc tggacagcga
      601 tttcatccgt gaatggtggt ggagcaacga cggctcccgc tggcgtgcgg accgcccgga
      661 tggcgacgtc ttcggaggac aatggggcga tggcacagcc gggctcaact acctggggcc
      721 ggatacggcc gactatcagg aatactacac gctcggtcgt agcgaacagg agaacccctg
      781 ggacgccctc gtgaccgtgt gcgacaaatt gaacaacacg cccatcgcgc aattgaacga
      841 taccctggac aaatacatgg acatcgaccg tgtcctgtgg catctggcgg gggagaacgc
      901 cttcggggat gacgacagtt acatccacaa gggcaagatg gactacagcc tctattggga
      961 cccggagacc ggtcggatga ccacgcaaga gcttgatggc aacagcatcc tgggcacgga
     1021 atcgctcaat tggagcccgt tctatcatgc gaacaacgtg aactacccct tgctcaaccg
     1081 ggtgctccaa gtacctgaac tgcggcaacg ttacctggcc cacctgcgcc acatcatagc
     1141 caccagcatg caacaggccg atgtagcggc cctcatcgcc caataccgct cgctgatcga
     1201 caccgtcgtc caggacgacc cgatcaaatt gatgacgtgg tcgcagtacc aatcctcatg
     1261 caccaccctg gggaacaggt tcaacaccag gcgcaacgca ctgaacaaca acaccgaggt
     1321 ggcccaacaa gcgccgacga tcggcaacgt ggagcacgag accggtgcgg gcatcgggca
     1381 tcccccggtc gccaacgaaa cggtacacgt gcgtgcaacg gtatcctcaa cgaacgggat
     1441 caacagggtg ggcttgtact ggagcaatgt cctgccgggg cgcttcagtc gtatggacat
     1501 gcacgacgat ggggcgcaca acgacgggag cgccggtgat ggcgtctttg gcgccgacat
     1561 tcccgggcag ccgggtggct tctgggtgcg ctactacgtg gccgccgagg cggacaacac
     1621 ggcacacagc accgccttct cgccacccgg tgccgaacac gacgtgtgga tctaccaggt
     1681 gacccccacc gtttcgccca cggaaggtgt ggtgatcaat gaactgatgg ccagcaacag
     1741 ttccacggtg atggacaaca acgaccagta cgaggactgg gtggaactgt acaaccacgg
     1801 ctccgccccg gtggacctgg agggatggtt ccttaccgat acgcccttcg aacccaccaa
     1861 gtgggaattc cccggcggta cggtgctggc ccccggcatg tacatgatcg tttggcccga
     1921 tgaggaccag agccagggcc tgatgcacgc gaacttcaaa ctcagcgcct cgggcgaaag
     1981 cgtactgctc tacgatccgg atacgctgct ggccgaccag gtggacttcg ggcagcagat
     2041 caccgatatg gggtatgccc gcgttccgaa cgggacggga cctttcgtga tccaggcgcc
     2101 gaccttcgcc gcgagcaacg acgtgaccgg attcgatgaa gtcgctccga atgccggctt
     2161 ccgcatgtac cccaacccgg ccagcacgca cgtgatcctc gtgtgggacg gggccggaac
     2221 gcataccgtg accatccacg acgctaccgg tcgactggta cgcaacctga tcttgaactc
     2281 cgggtcgatc ctggaaacaa gcacgttcgg gccgggcacc tacacggtac gctgcgcgca
     2341 ggccacccaa cgactggtgg tggtgcactg aacggccacc gctgcgcgtt tggcctccag
     2401 ttgtgggcag tgccgctcct gatgcgcgac ggggaagcgg tgcgcgacct gcgcacccgt
     2461 tccatcaggc atgccggtcg gcgagcaatg cccgtgtgtg atcatacccg atccgctgga
     2521 tccgggcgga atgactcagg tcgaacatcc cgtactcggc caggcccggt ggctcaatga
     2581 acatgcggca tcccttggct gaacgcgtga ccatctcgcg gaaactgagg tggaaggtgc
     2641 ggtccagcgt gcggcgcacg gtccgctgag cggaataggg aggtagcgga ttcacgtaca
     2701 ccgcgatcac ttcggaccgg cgaccctcaa agggttccac gggcagattg ttgctcagcc
     2761 caccgtccac gtacggtgtc ccatcgatca caacgggcgg aaagatgagc ggtacggcac
     2821 aggccgccag caccgggggg atgatctcgc ccgcgctgaa gatccgctgc ccgccatgct
     2881 ccaggtccgt ggcactcacg tacaacggga cccgcagatc ctcaaaatac ttcaccggaa
     2941 ggtatttttc caggaattcg ccgatacgtc gctgggtgag cagctcgccg cgcaacaact
     3001 tccagcgcgt gcggtgcaac tgccattcct gatgaagtag cgctgaggcc tccatgggcg
     3061 tgagtcctgc cgctatgaag gcaccgatga gcgaacccgc gctcgttgca ctgatggccg
     3121 agggcccgat gcccgcctcg tggaaagcct gcagcacgcc cagatgcgcg atgcccctcg
     3181 caccgccacc ggagagaacg aaggggacgt gttccatcgg cgcgaagatc ggtggaggag
     3241 gctttccacc accttctccg cacgcacctc cttacgggaa tgcaggcagg ccgatcgagg
     3301 tgctgcgacc g
//
LOCUS       DAOQOC010000194         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14453_length_3294_cov_3.134918, whole genome shotgun sequence.
ACCESSION   DAOQOC010000194 DAOQOC010000000
VERSION     DAOQOC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14453_length_3294_cov_3.134918"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PL106_04220"
     CDS             <1..446
                     /locus_tag="PL106_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32308.1"
                     /translation="YVANNTGAGLDIAPGGLDWSGAWPQNWLYFAGESAGCLYLSASC
                     RAFQKVHITYSGLGIDCLGDDFRIPEWAREAITDAVILRGAKGLRAITRDNLYREIIR
                     EKEPQLALTNPNGTWLRALGHWGRMGQKDRMDTWTRTTWFGYPPY"
     gene            448..>3294
                     /locus_tag="PL106_04225"
     CDS             448..>3294
                     /locus_tag="PL106_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32309.1"
                     /translation="MPDLQHSDRFTRWFRRLFGGGAQPDVRPELIGEGAFTDALNLRI
                     GSVMGNTGALETVGGETLLWDAPQGLGVDTSSYVCIGTASGNGYVVEFWASTLWDSGT
                     GANPPIIRANGTVVAMSRHIPYEATWPLQIATVLNCGERIMLLFPVDHHSDPLYWDVK
                     DMLDHLALGDGFYFENYTTDINSVGFAAAPSIPWPKYPRPVSDLGSAIGLPHGQYNYF
                     LRYVTVGGDVTNWGPPTGNIPIPAMLGAAGGVHNRTNGGTPDEQAPSRYGITIEFRVD
                     NTAGFRAVQIGRQRFNDGGGIQGVGVKEIVAEIELQYGQTGILSFSDPGEVNIVPAQT
                     IAADEAAQQVMTIHAPKSVDYSDSRLKYANANLDPKKIELTFGSLSTGKKFTAITKAL
                     RRLVGGEWLNEGYEEPYHWAVNKSAFRGGRTKYGVQFWDGANGLSPAVNIPEPWEPGH
                     NDMVFPQRRDTKGDNPALAPQGKNWGPESSVYSDDPCYAANVDVASQGPRGPVTPTFE
                     AFTSGEKAKNDMATYINVIKRTDEPAPTGMPVRLTGQYNMFSNAFGGNTMSVDGGAGT
                     SPTQYKPWRPSNNADGETYGYNVPPNVARNVGSANDPNVVNQCNFNGINNAGACFDPC
                     HHALGSLFAGVTGGIPASARIMSIVYDGDTRGEVVAQGFGCWDLANNYYGGGGGDNEG
                     DIERYLPARKRTNGLRVFFPDVHSGVVDQAIIDDIIANPQNYQIRMVSPVPFYSDFYG
                     GVLHHVDSPVWPGQSNSFNLDPNGQTMLMDVMCHTSFLHDEGRVNVGTQPAGNEGYQP
                     TGGEAPDGNWIGFGQWRWPGSNIPAIWNQFATGAYPMQGGNGLFTINQLTPITNGRAA
                     FYQLVTDQYIYDVDDLQVGGTVYTGFNDDEVRKFQQPWYALNIVRSGYVQPDASIQPY
                     KNMGVHIALDTNLGAYNGGIQDERLLGEREKHDCT"
BASE COUNT          677 a         1086 c         1048 g          483 t
ORIGIN      
        1 ggtatgtggc gaacaacacc ggcgcggggc tggacatcgc gccgggcgga ctggactggt
       61 ccggcgcgtg gccgcagaac tggctgtact tcgccggaga gagtgccgga tgcctttacc
      121 tgagcgccag ttgccgcgcc ttccagaagg tacacatcac ctactcgggt ctgggcatcg
      181 actgcctggg ggatgacttc cgcatacccg aatgggcgcg ggaggccata accgatgcgg
      241 tcatactgcg gggcgccaag gggttgcgtg ccatcacacg ggacaacctc taccgggaga
      301 tcattcgcga gaaagagccg cagttggccc tgacaaaccc caatggcacg tggctccgcg
      361 cgctggggca ctggggtcgc atgggacaga aggaccgtat ggacacgtgg acgaggacga
      421 cctggttcgg ttatccgccc tactgaaatg cctgatctgc aacatagcga ccggttcacc
      481 cgctggttcc gccgcctgtt cggtgggggt gcccagcctg acgtgcggcc cgagttgatc
      541 ggcgagggcg ccttcaccga cgcgctcaac ctgcgcatcg gcagcgtgat gggcaacacc
      601 ggcgcgctgg agaccgtggg cggcgaaacg ctcctgtggg acgccccgca ggggttgggc
      661 gtggacactt ccagctacgt gtgcatcggc acggccagcg gcaacggcta cgtggtggag
      721 ttctgggcca gcaccctttg ggacagcggc accggagcca acccccccat catccgcgcc
      781 aacggcacgg tggtggccat gtcgcgccac atcccttacg aagccacatg gcctttgcag
      841 atcgccacgg tgctcaactg cggggagcgc atcatgctcc tcttcccggt ggaccaccac
      901 agcgacccgc tgtactggga cgtgaaggac atgctggacc acctcgcgct tggggacggc
      961 ttctacttcg agaactacac cacggacatc aacagcgtgg gcttcgccgc cgcgcccagc
     1021 ataccgtggc ccaagtaccc gcgtcccgtg agcgacctcg gcagcgccat cgggttgccg
     1081 cacggccagt acaactactt cctgcgctac gtcaccgtgg ggggtgacgt gacgaactgg
     1141 gggccgccca cgggcaacat ccccatacca gccatgctgg gagcggccgg gggcgtgcat
     1201 aaccgcacca atggcgggac gccggacgag caggcaccct cgcgctacgg catcaccatc
     1261 gagttccggg tggacaacac cgccgggttc cgcgccgtgc agatcgggcg ccaacgcttc
     1321 aacgacggcg gcggcatcca aggggtcggc gtcaaggaga tcgtcgccga gatcgaactc
     1381 cagtacggac agaccggcat cctctccttc agcgaccccg gggaggtcaa catcgttccg
     1441 gcgcagacca tcgcggcgga cgaggcggcg cagcaggtga tgacgatcca tgcccccaag
     1501 agcgtggatt attcggacag ccgcctgaag tacgccaacg ccaatctgga ccccaagaag
     1561 atcgagttga ccttcgggag cctcagcacg ggcaagaagt tcacggccat caccaaggcc
     1621 ctgcgcaggc tggtgggcgg ggagtggctg aacgagggct acgaagaacc ctaccactgg
     1681 gccgtgaaca agagcgcgtt ccggggtggt cgcaccaagt acggcgtgca gttctgggac
     1741 ggcgccaatg gcctcagccc cgccgtgaac atccccgagc cttgggaacc gggacacaac
     1801 gacatggtgt tcccccagcg ccgcgacacc aagggggaca accctgcact ggccccccaa
     1861 ggaaagaact ggggaccgga aagctccgtg tacagtgacg atccctgcta cgcggccaat
     1921 gtggacgtgg cctcacaagg gcctcgtgga cccgtcacac cgacgttcga ggcgttcacc
     1981 tccggggaga aggccaagaa cgacatggcc acgtacatca acgtcatcaa gcgcaccgac
     2041 gagccagccc ccacagggat gcccgtgcgc ctcaccgggc agtacaatat gttctccaac
     2101 gccttcgggg ggaacaccat gagcgtggat ggcggcgccg ggacaagccc cacgcagtac
     2161 aagccgtggc gccccagcaa caacgccgac ggcgagacct acggctacaa tgtgcccccg
     2221 aacgtggcac gcaacgtcgg ctcggccaat gaccccaacg tggtcaacca gtgcaatttc
     2281 aacgggatca acaacgccgg ggcctgcttc gacccctgcc accacgcgct gggttcattg
     2341 ttcgctgggg tgacaggcgg catccccgcc agcgcccgca tcatgtccat cgtgtacgac
     2401 ggggatacac ggggggaggt cgtggcgcaa gggttcggat gctgggacct cgccaacaac
     2461 tactacgggg gaggcggggg tgacaacgag ggcgacatcg aacgctacct cccggcacgc
     2521 aagcgcacca acggactgcg cgtgttcttc ccggacgtgc atagcggggt ggtggatcag
     2581 gccatcatcg acgacatcat cgccaacccc cagaactacc agatccgcat ggtcagcccc
     2641 gtgccattct actcggactt ctacgggggg gtgctgcacc acgtggacag ccccgtgtgg
     2701 cccggacaaa gcaattcgtt caacctggac cccaacggcc agacgatgct catggacgtg
     2761 atgtgccaca cgagcttcct gcacgacgag ggaagggtca atgtgggcac gcagccggcg
     2821 ggcaacgagg gataccagcc aactggcggc gaggcgcccg acggcaactg gatcggcttc
     2881 ggccagtggc gctggcccgg ctccaacatc ccagcgatat ggaatcagtt cgccaccggt
     2941 gcctacccca tgcaaggagg caacggactg ttcaccatca accagcttac gcccatcacc
     3001 aacggcaggg ccgcgttcta ccaactcgtg accgaccagt acatctacga cgtggatgac
     3061 cttcaggtgg gcggcacagt ctacaccggg ttcaacgacg acgaggtgcg caagttccag
     3121 cagccgtggt acgcgctgaa catcgtgcgc tcaggatacg tccagcccga tgccagcata
     3181 cagccctaca agaacatggg cgtccacatc gcactggata ccaacctggg cgcgtacaac
     3241 ggcgggatcc aagacgagcg cctgctgggt gaacgcgaga agcacgactg cacg
//
LOCUS       DAOQOC010000195         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14534_length_3285_cov_3.774303, whole genome shotgun sequence.
ACCESSION   DAOQOC010000195 DAOQOC010000000
VERSION     DAOQOC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14534_length_3285_cov_3.774303"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..113)
                     /locus_tag="PL106_04230"
     tRNA            complement(41..113)
                     /locus_tag="PL106_04230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Ile,seq:cat)
     gene            167..796
                     /locus_tag="PL106_04235"
     CDS             167..796
                     /locus_tag="PL106_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNA32310.1"
                     /translation="MIDTLLLDLGGVLIDVDYHRTADAFNALGFDGFEAIYSKAQQDH
                     LFDGFEIGALSPEEFRARIRSLHGNGITDAQVDHCWNAMLGRIPPERIDLLKRLRTRY
                     RLLLLSNTNAIHVPAFQRIIADHNGINDLKALFDGAYYSCDIGLRKPEAEAFHHVLGR
                     HGAEPARTLFIDDSMQHVIGARAAGLQAEHLDLHKEDLLQFVRRRGLMD"
     gene            complement(797..1135)
                     /locus_tag="PL106_04240"
     CDS             complement(797..1135)
                     /locus_tag="PL106_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNA32311.1"
                     /translation="MATRRSTAEFILEQLGHADRFSVRPMFGEFALYADGKPVAFICD
                     DQLLVKILPESAPLEARCDRAPAYPGSKDYYLVPEDMIAGERKLPDMLLRMASVLPLP
                     KNRKKPARRT"
     gene            complement(1139..1540)
                     /gene="mce"
                     /locus_tag="PL106_04245"
     CDS             complement(1139..1540)
                     /gene="mce"
                     /locus_tag="PL106_04245"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNA32312.1"
                     /translation="MIRIEHLGIAVRDLDQAEATWTKLLGRAPYKREEVEREGVITSF
                     FQVGPNKIELLESTRPDGPVAKAIEKRGEGIHHVAYEVEDIRAEMVRLKAEGFQLLSD
                     EPKLGADNKLVCFVHPKGATGVLVELTQEIR"
     gene            complement(1660..2448)
                     /locus_tag="PL106_04250"
     CDS             complement(1660..2448)
                     /locus_tag="PL106_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32313.1"
                     /translation="MRAPILTLSVLLGLTASAGTIVLEGNYQGKNLFIQNPFSEAGVG
                     FCVYEVTVNDQVATDEINSSAFEVDLANFGLKIGDKITVKIKHKDGCVPLVLNPEVLR
                     PKSTFDIVRQQVSADGTYTWITNNETGELPFVVEQKRWNKWVKVGEVPGLGTPGEHTY
                     NFKVIPHSGENTFRIKQVDLTKKARYSQVVTFTDPSVAPITWSPQKPKDEIVFSGNTL
                     YEVYDQYGNIVKRGYTNKLDVSTLKKDLYYLNYDNKMGETFIKR"
     gene            complement(2566..3084)
                     /locus_tag="PL106_04255"
     CDS             complement(2566..3084)
                     /locus_tag="PL106_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32314.1"
                     /translation="MKNTVLLSGMVLFFACSSGPTEEQETRVADTTTVAHIDLTSYGL
                     PLLLTPPDKQLTAGAEPSIIWKDETGTLAVEAGERFGLEIREEPGDMPRLKATLERDL
                     LRKNTIIRETPELLVWRSEFPDDPDLVSIHFYRILAADGRTFTVQDSDGPAYSEQDVD
                     RMSAAISTRSPA"
BASE COUNT          617 a          943 c         1002 g          723 t
ORIGIN      
        1 gagaacgatc cgccttcgct aaagtttcgg cggacgaatg tgggcccagc aggatttgaa
       61 cctgcgacca agggattatg agtcccctgc tctgaccgct gagctatagg cccggataat
      121 ggtgcgcgaa agtaaagggt ggtcgttgcg gatagcttcg cgcgccatga tcgacacttt
      181 attactggac ctcggtggcg ttctcatcga cgtggactat caccgtacgg ccgatgcctt
      241 caatgcactg ggcttcgatg gcttcgaggc gatctacagc aaggcccaac aggaccattt
      301 gttcgatggc ttcgagatcg gagcgctttc cccggaggag ttccgtgcgc ggatccgcag
      361 ccttcacggg aatggcatta cggacgcgca ggtggaccat tgctggaatg ccatgctcgg
      421 tcgcatccca ccggagcgga tcgacctgct caagcggctg cgcacccggt acagactgtt
      481 gctgctcagc aatacgaacg cgatccatgt gccggccttc cagcggatca tcgcggacca
      541 caacggcatc aacgatctca aggcgctgtt cgatggggcg tactacagtt gcgatatcgg
      601 gctacgcaag ccggaagccg aggccttcca tcatgtgctc ggtcggcacg gcgcagaacc
      661 cgcgcgcacg ctgttcatcg atgacagcat gcagcacgtg atcggtgcac gagcggcagg
      721 cctgcaggcc gagcacttgg atctgcacaa ggaggatctg ctccagttcg tccgacggcg
      781 gggattgatg gattgatcac gttcgccgcg ctggtttctt acggtttttc ggcagcggca
      841 ggacgcttgc catacggagc agcatatctg gaagcttgcg ctcgccggcg atcatgtctt
      901 cgggcaccag gtaatagtcc ttcgagccgg gataggccgg ggcccgatcg caacgcgcct
      961 ccaatggcgc gctttccggc aggatcttca cgaggagctg gtcatcgcag atgaaggcca
     1021 ccggtttgcc atcggcgtac aacgcgaact ccccgaacat ggggcgcaca ctgaaccggt
     1081 cggcgtgtcc caattgctcg agtatgaact cggccgtcga tcttcgggtg gccatgacct
     1141 accggatctc ctgcgtcagt tccaccagaa cccctgtggc gccttttgga tgaacgaagc
     1201 agacgagttt gttgtcggcc ccgagcttgg gctcatcact caacagctgg aaaccttcgg
     1261 ctttcaaacg caccatttcc gcacggatgt cctccacttc gtaggccacg tggtggatgc
     1321 cctcgccacg cttttcgatg gccttggcaa cagggccgtc cggccgggtg ctctcgagca
     1381 gttcgatctt gttcgggccc acctggaaga aggaggtgat cacaccttcg cgttccactt
     1441 cctcgcgctt gtatggtgca cgccccagca gtttcgtcca ggtggcttcg gcctggtcca
     1501 ggtcccttac ggcgatgccg aggtgttcaa tgcggatcat ggtgtcggat attgctgagg
     1561 aggcgtgggg atgcagaggt acattgaagc tcccgtaccc tgcatggtgt catgaaaaag
     1621 ccccaccaaa gcggggcttt ccaaggcgat cccgggcgtt caacgcttga tgaacgtttc
     1681 gcccatcttg ttgtcgtagt tcaggtagta caggtccttc ttcagcgtac tcacatcaag
     1741 cttgttggta tacccgcgct tcacgatgtt cccgtactgg tcgtacacct cgtaaagcgt
     1801 gttgccgctg aagacgatct cgtccttcgg tttttgaggg ctccatgtga tgggggccac
     1861 cgaggggtcg gtgaatgtga ccacttggct gtaccgcgct ttcttggtga ggtcgacctg
     1921 cttgatgcgg aaggtgttct ccccgctgtg gggtatcacc ttgaagttgt acgtgtgttc
     1981 ccccggagtt cccaagccgg ggacttcgcc cactttcacc cacttgttcc agcgtttttg
     2041 ttccaccacg aacggcaatt caccggtctc gttgttggtg atccatgtgt aggtaccatc
     2101 ggcggacacc tgctgtcgta cgatatcgaa ggtgctcttg ggccgtagta cctcgggatt
     2161 gaggaccagg ggaacgcagc catccttgtg tttgatcttc acggtgatct tgtcgccgat
     2221 cttcaggccg aagttggcca agtccacttc gaaggcactg gagttgatct cgtcggtggc
     2281 aacctgatca ttcaccgtca cctcatacac gcagaaaccg acacccgcct cagaaaaggg
     2341 attctggatg aacaggttct ttccttggta attgccctcg agcacgatcg ttcccgccga
     2401 cgcggtaaga ccgagcagga cggacagggt caaaattgga gcacgcatcc agattgggcg
     2461 gatatggggt cgcaatgtta gcaattcgcc ggggcatgga caaagtgacc gggtcattct
     2521 gaacccgctg tgcatccgcg agttgggccc gacacgggga tcttctcagg caggggatct
     2581 cgtggatatc gctgcggaca tgcggtccac atcctgttcg ctgtacgcgg gtccgtccga
     2641 gtcctgaacc gtgaatgtac gcccgtccgc cgcgaggatg cggtagaaat ggatggacac
     2701 gaggtccgga tcatcgggga attcgctacg ccagaccagg agttcagggg tttcgcgaat
     2761 aatggtgttc ttgcgcagca ggtcgcgctc gagtgtggcc ttcaagcggg gcatgtcgcc
     2821 gggttcctcg cggatctcca aaccaaaacg ctcgcccgcc tccacggcca gggtacccgt
     2881 ttcatctttc cagatgatgg agggctccgc gccagcggtc agttgtttgt ccggaggggt
     2941 cagcagaagg ggtagcccgt atgaggtcag gtctatatga gcgacagtgg tggtgtcggc
     3001 gacgcgggtc tcctgctcct cggtcggacc gctgctgcac gcgaagaaca ggaccatacc
     3061 cgacaataga acggtgtttt tcatgggggt gcgaaattgc cggacatgcc ctcccgttcc
     3121 gatccgggag agcaggttct ctccacttat cacggtggga tcggcggcct ccggccgggc
     3181 cgggatcagg gcatgaacat ttcgagcatg ctgaaaagag cacccgtgat gtgcccgatg
     3241 gccagggccg gttccgcaag catcggtcgt cgctgttgcc agcct
//
LOCUS       DAOQOC010000196         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14545_length_3283_cov_4.186803, whole genome shotgun sequence.
ACCESSION   DAOQOC010000196 DAOQOC010000000
VERSION     DAOQOC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14545_length_3283_cov_4.186803"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PL106_04260"
     CDS             complement(<1..541)
                     /locus_tag="PL106_04260"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNA32315.1"
                     /translation="MPRIGPIELPEFPLLLAPMEDVSDPPFRALCKKHGADLMYTEFI
                     SSEGLIRKAAKGMKKLDIFPEERPIGIQLFGGSEDAMEEAARIAEAAGPELIDINYGC
                     PVNKVVNKGAGACLLQDVDKMVRLTEKVVKAVKLPVTVKTRLGWNDHTKNIVEVAERL
                     QDVGIAALSIHGRTRAQLYK"
     gene            821..1231
                     /locus_tag="PL106_04265"
     CDS             821..1231
                     /locus_tag="PL106_04265"
                     /inference="COORDINATES: protein motif:HMM:NF018837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HNA32316.1"
                     /translation="MEEHLSERNNDRLVNLMTIARTHEGSEYDLDVMNELLYGSAMLL
                     VPAKASEEMEEGDEGTTMSIDAPSIDGVLTLYAFTGEAAVRSFATEEITCMGLPSSEL
                     LRICMQQGVGAILLDPDTPNELCVTLSPFRSGEN"
     gene            1301..1390
                     /locus_tag="PL106_04270"
     tRNA            1301..1390
                     /locus_tag="PL106_04270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1335..1337,aa:Ser,seq:gga)
     gene            complement(1751..2782)
                     /locus_tag="PL106_04275"
     CDS             complement(1751..2782)
                     /locus_tag="PL106_04275"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HNA32317.1"
                     /translation="MSERSRVLIIYTGGTIGMMADPRSGALLPMDMAHLELHVPELER
                     IDVDLDAVSFERPIDSSDMRPADWITMARIIGERYDRHDGFVVLHGSDTMAYTASALS
                     YLLEGLGKPVVLTGSQLPIGTIRTDGKENLLTAIEIAAANEGGRPIVSEVAVYFEYAL
                     FRGNRTVKVHAERFEAFRSPNWPLLAEAGVHLRYDRQAMLPRRNGPLHVHERLNENVG
                     VIPLFPGIRADWVCHALSTPGMRAVVLTTFGSGNGPTDTAFLKALHEARERGIVLVNV
                     TQCVGGRVEQGRYVTSQAFAEMGVVSGHDLTVEAAITKLMFLFGQELSAEEVARHVQV
                     PLCGEMSVS"
     gene            complement(2779..>3283)
                     /locus_tag="PL106_04280"
     CDS             complement(2779..>3283)
                     /locus_tag="PL106_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNA32318.1"
                     /translation="HAVGEIGIDLYWDKSRLIQQEEVFLTQVRWAKELSLPVVIHCRD
                     SFDEVFRIVEDENDTHLRGVFHCFTGTAEQARLITGLGGFMLGIGGVITYPKSGLAQV
                     MAEVGADHCVLETDAPYLAPVPHRGKRNESTYVPIVAQALAQATGSTLDDIARMTTAN
                     AEKLFAA"
BASE COUNT          592 a         1066 c         1021 g          604 t
ORIGIN      
        1 ccttgtacaa ctgcgcccgt gtgcggccat ggatgctcag cgcagcgatg cccacgtcct
       61 gcaaccgctc ggccacctcc acgatgttct tcgtgtggtc attccagcca aggcgcgtct
      121 tcaccgttac cggcagcttc accgccttca ccaccttctc cgtcaggcgc accatcttgt
      181 ccacatcctg caacagacag gcgcccgctc ccttgttcac cactttgttc accgggcagc
      241 cgtagttgat gtcgatcagc tcaggccctg cggcctcggc gatgcgtgcg gcctcctcca
      301 tggcgtcctc gctccctccg aagagttgga tgccgatggg ccgctcctcc gggaagatgt
      361 cgagcttttt catgcccttg gccgccttgc ggatcaaccc ttcgctgctg atgaactcgg
      421 tgtacatcag gtcggccccg tgctttttgc agagcgcccg gaatggagga tcgctcacgt
      481 cctccatggg tgccagcaac agcgggaatt ccggcagttc gatggggccg atgcggggca
      541 tggaataggg agtgcgaaag tacggaacac ccgccgggcg tacctcagcg gcgggacctg
      601 cgcggaaagc cgctcggggc gggcgctgaa cccggcacat gcgttccgcg ttgaagcggg
      661 cggcactcgg gtggtactgg ccgcctgcgc gcagaagatc cgaacatgcc caagccacga
      721 accgctgcct ggaacgtcta ccggatgaaa ggtctaccgc aggcgtgttc gcaccgacca
      781 ccgaacggaa ccgtggactg aaccgatcaa cgcgctcatc atggaagaac acctgtccga
      841 acgcaacaac gaccgcctgg tgaacctgat gaccatcgcg cgcacgcacg agggatcgga
      901 gtatgacctg gacgtgatga acgaattgct ctacggttcg gccatgttgc tcgtgccggc
      961 caaggccagc gaggagatgg aggaagggga tgaaggaacg accatgtcga tcgatgcgcc
     1021 ttccatcgac ggggtgctca cgctctatgc cttcaccggc gaggccgcgg tgcgcagttt
     1081 cgccacggaa gagatcacgt gcatgggctt gccttccagc gaactgctcc gcatttgcat
     1141 gcagcagggt gtgggagcca tcctcttgga ccccgatacc ccgaacgagc tgtgtgtgac
     1201 cttgtcgccg ttccgctcgg gggagaactg atcctaccgg tcgaacgcgt tgcccgctcc
     1261 gtcagcggga gccccctttg cctactttcg ccgcccctcc ggagagatgg ccgagtggtt
     1321 taaggcgcac gcctggaaag tgtgtttacc tgaaagggta acgggggttc gaatccctct
     1381 ctctccgcca atgggaaggc cgccgaaagg cggctttctt gttcgagatg cgagcggagc
     1441 atgctccagc gagcggatcg aacaggaaag cgcagcgcga agcgatgcgg cctgagcatt
     1501 gaagccaccc ccaccgggat gccctgccat ccctctctct ccgccaacgg gaaggccgcc
     1561 gaagggcggc tttcttgttc gagatgcgag cggatcgaac gggaaagcgc agcgcgaagc
     1621 gatgcggcct gagccttgaa gccaccccct tgaagaaagc gcgcttcgca cgatcccgat
     1681 cccgttggcg aaaacgtgct ggtcgacccg agcccgggtt aaaaaaatac ccgatcacgt
     1741 gtcctgctgt tcaggatacg ctcatctctc cgcacagggg cacctggacg tgtcgggcca
     1801 cctcttcggc ggaaagctcc tgcccgaaca ggaacatgag tttggtgatg gccgcctcca
     1861 cggtcaggtc gtgtccgctc accaccccca tctcggcgaa ggcctggctg gtgacgtagc
     1921 gcccctgttc cacgcgtcct cccacgcatt gcgtgacgtt caccagaacg atgccccgtt
     1981 cgcgcgcttc gtgcagggcc ttcagaaaag ccgtatcggt ggggccgttg ccgctgccga
     2041 aggtggtgag gaccacggcg cgcatgccgg gcgtgctcag ggcatggcac acccagtcgg
     2101 cgcggatgcc cgggaacagg gggatcaccc cgacgttctc attcagccgc tcatgtacat
     2161 gcagcggacc gttgcgccgg ggcaacatgg cctgccggtc gtagcggaga tgcacgccag
     2221 cctcggccag caggggccag ttgggcgagc ggaaggcctc gaaacgttcg gcatgcactt
     2281 tcacggtccg gttgccccgg aagagcgcgt actcgaaata gaccgctact tcgctcacga
     2341 tcggcctgcc gccctcgttg gccgcagcga tctcgatcgc ggtcagcagg ttctccttgc
     2401 cgtcggtgcg gatggtgccg atgggcagtt gcgagccggt gagcaccacc ggcttgccca
     2461 acccttcgag caggtagctg agcgcactgg cggtgtaggc catggtgtcg ctgccgtgca
     2521 acaccacgaa gccatcgtgt ctatcatacc gttctccgat gatccgcgcc atggtgatcc
     2581 aatccgctgg gcgcatgtcg ctgctgtcga tgggccgctc gaacgacacg gcatcaaggt
     2641 ctacgtcgat gcgttccagc tcgggtacat gcaactcaag atgcgccatg tccatgggca
     2701 gcaaggcccc ggagcgcgga tcggccatca tgccgatggt gccccccgta tagatgatca
     2761 gtacgcgcga acgttcgctc atgcggcaaa gagcttttcg gcgttggccg tggtcatgcg
     2821 cgcgatatcg tccagcgtac tgccggtggc ctgcgccagg gcctgggcca cgatcggcac
     2881 gtaggtgctc tcgttgcgct tgccgcgatg gggcacgggg gccaggtagg gcgcgtcggt
     2941 ctccagcacg caatgatccg ctcccacctc ggccatcacc tgcgccagcc cgctcttggg
     3001 gtaggtgatc actccgccga tgccgagcat gaaaccaccg aggcccgtga tcagacgcgc
     3061 ctgctccgcc gtgcccgtga agcaatggaa cacgccgcgc aagtgggtgt cgttctcgtc
     3121 ctcgacgatc cggaacacct catcgaagga gtcccggcaa tggatcacca caggcagtga
     3181 tagttctttg gcccagcgca cctgggtgag gaatacctcc tcctgttgta tcagccgcga
     3241 cttgtcccag tacaggtcga tgccgatctc gcccacggcg tga
//
LOCUS       DAOQOC010000197         3278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14578_length_3278_cov_3.765436, whole genome shotgun sequence.
ACCESSION   DAOQOC010000197 DAOQOC010000000
VERSION     DAOQOC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14578_length_3278_cov_3.765436"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PL106_04285"
     CDS             complement(<1..578)
                     /locus_tag="PL106_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32319.1"
                     /translation="MSLLLTWTATAQSRKDLEKKRDQLDKQIKTTSALISAGEREQRA
                     TQRQLELLQAQIRQRQELIGTMNSEVFRVDKEIGETEELIEALGSDLARLKEEYARML
                     QYAYMNRDTYDRLSYLFAARSFTQAFQRSRYLDQLADRRRQQAALITDTQASLERRAD
                     DLKNRRTEKVSLLNEQVSEREKLSADRGAHEST"
     gene            complement(643..1506)
                     /locus_tag="PL106_04290"
     CDS             complement(643..1506)
                     /locus_tag="PL106_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNA32320.1"
                     /translation="MGCLLLLAACKPIRRVLPFRGDLPPRGAEKMMEHLSAARITDVR
                     YYSAKSSAEVSDSSGTRSFKAHIRCVMDSAAWVSIVPVLGIELARVLLTPDSLKVMDK
                     FHDTYWTGDTAMAKKRFGLAPDLSLLQQALLGQPIAFDPTDRYRSDREDGQYTLTSRE
                     KRRFRRAAEDLAEGDTIPRGHDISERRLERTLRRAEKKDAFVVKYWLEPDSFLVTRVL
                     ISDLAHDQQADVRYSARTPVNSHSLPTRVAISLSDPTRHAEAVLELDRISLDGPLQLN
                     FRIPEKFKPLE"
     gene            complement(1530..>3278)
                     /locus_tag="PL106_04295"
     CDS             complement(1530..>3278)
                     /locus_tag="PL106_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32321.1"
                     /translation="TLFVPLLAVLLACGGAKPVAEAPRATDGVGTAQDASFKSKRALV
                     MRLFMEANQARLRGELPKAIQLYEATLKEDPRNAASMFELGKLYNQAQAAAPAMAMAK
                     RAVATDKENIWYRFLVADLQLQENDLAGASRTYQDLLAKWPDRYEVYFGLANTLAEQK
                     RIGEARQVFRDLEKRIGSNEELVMREYDMLANAGETAAARDLLDKAIKEQPGQTQYYG
                     MLAEVYDSMGDSTKAEALYKKALELDPDDSMTRISLAQFYYDHNRIEEGFAHLKEAFG
                     DPDLDIDPKMHLLLGFYESGGQDGKLPEDLARQCHGLIDELKKAHPQSGKPWSIEGDF
                     YSREGNIPGARDAFAKAAEFDPDRYPIWSALVQLDARLNDQRSMNEHAQRAIELFPAQ
                     PEFYLYKGIAASGLKRYDEAIEALVTGRDLVVDNKPLEGQFWSALGDAYNAAREFSKS
                     DEAYDRALSIDGDDATVLNNYAYYLSERGEQLDKAEKMSRRCNDLVPGTATYLDTYAW
                     ILFKQGKFADARTWQEKALAAGGDTEGVLVEHYGDILFKLGDAEGALTQWRKAQGIGD
                     ASELVGRKITEGRYIE"
BASE COUNT          512 a         1017 c         1036 g          713 t
ORIGIN      
        1 gtgctttcgt gggcgccgcg atccgctgaa agcttttccc gctcgctcac ctgctcattc
       61 aagaggctca ccttctcggt gcgacggttc ttgaggtcgt cggccctacg ctccaagctt
      121 gcttgggtgt ccgtgatcag tgcggcctgc tggcggcgcc ggtcggccag ttggtccagg
      181 tagcggctcc gctggaaagc ctgtgtgaaa ctgcgggcgg cgaaaagata actcaggcgg
      241 tcgtacgtgt cccggttcat gtaggcgtac tgcagcatac gcgcatactc ttccttcagc
      301 cgggcgaggt cggaacccag ggcctcgatg agctcttcgg tctcgccgat ctccttgtcc
      361 acgcggaaca cctcgctgtt catcgtgccg atcagttcct gccgctgacg gatctgggct
      421 tgcagcagtt ccagttggcg ttgcgtggcg cgttgttccc tctccccggc ggagatcagg
      481 gcgctggtgg ttttgatctg tttgtccagc tggtcgcgct tcttttccag atccttgcgc
      541 gattgggccg ttgccgtcca tgtgagcaac agcgacaaga accacagcgt cgttctcgaa
      601 ctggagcggt ggcacaacgc gcgatccgtg gcccggagcg cctcactcca agggcttgaa
      661 cttctccggg atcctgaagt tgagttggag tgggccatca aggctgatcc ggtcgagttc
      721 gaggacggct tcggcatgcc gcgtggggtc ggacaaagaa atggcgacac gcgtgggaag
      781 cgagtggcta ttcacgggtg ttcttgctga ataacgcacg tcggcttgtt gatcgtgcgc
      841 gaggtcgctg atgagcacgc gggtcaccag gaatgaatcg ggctccagcc agtatttgac
      901 cacgaatgcg tcctttttct ccgcacgtcg cagggtgcgt tcgaggcgac gttcgctgat
      961 gtcgtgaccg cgtgggatgg tgtcgccctc ggccagatcc tccgcagctc gccggaagcg
     1021 ccgtttttca cgactggtga gggtgtattg gccatcctcc cgatcgctcc tgtatctatc
     1081 ggtcgggtcg aaggcaatgg gctggcccag gagggcctgc tgcagcaggg agaggtcggg
     1141 agcgaggccg aagcgcttct tcgccatggc cgtatcgccc gtccaatagg tgtcgtggaa
     1201 cttgtccatc accttcaacg agtcgggggt caggagcacg cgggccagtt cgatgccgag
     1261 caccggaacg atactgaccc atgcggcgct gtccatcacg cagcggatat gggccttgaa
     1321 actcctggtc ccggaactgt ccgagacctc ggccgagctt ttcgcactgt aataccgcac
     1381 atcggtgatg cgtgccgcgg agaggtgctc catcattttt tccgctccgc gtgggggcag
     1441 gtccccacgg aaggggagca ccctgcggat gggcttgcag gctgcgagga gcaagaggca
     1501 tcccattccg atggcccccg ccgcacgcat cattcgatgt accggccttc ggtgatcttt
     1561 cttccgacca gctcgctggc gtcgccgatc ccctgggcct tgcgccactg ggtcagcgcc
     1621 ccctcggcgt cgccaagctt gaagaggatg tcgccgtaat gctccaccag cacgccttcg
     1681 gtatcgcccc cggccgcgag ggctttttcc tgccatgtgc gggcatcggc gaatttgccc
     1741 tgcttgaaca ggatccacgc ataggtgtcc aggtaggtgg cggtgcccgg caccaggtcg
     1801 ttgcaccggc ggctcatctt ctccgccttg tccaactgct ccccgcgctc gctcaggtaa
     1861 taggcgtagt tgttgagaac ggtggcatcg tcgccgtcga tcgagagggc ccggtcgtac
     1921 gcctcatcgc ttttgctgaa ctcgcgtgcg gcattgtacg cgtcgcccag tgcgctccag
     1981 aactgtcctt cgagcggctt gttgtccacc acgaggtccc ggccggtgac gagcgcctcg
     2041 atggcctcat cgtatcgctt gagcccgctg gcggcaatgc ctttgtacag gtagaactcc
     2101 ggttgtgcgg gaaagagctc gatggcccgc tgggcatgct cgttcatgga tctttggtcg
     2161 ttcaagcggg cgtccagctg cacgagggcc gaccagatgg gatagcggtc cggatcgaat
     2221 tcggccgcct tggcaaaggc atcgcgcgcg ccaggtatat tcccttcgcg tgagtagaaa
     2281 tcaccctcga tgctccaggg cttcccgctc tgggggtggg ccttcttgag ttcgtcgatg
     2341 aggccatggc attgccgggc gagatcctcc ggcaatttgc cgtcctgtcc cccggactcg
     2401 taaaatccga gcagcaggtg catcttggga tcgatgtcca ggtcggggtc gccgaacgct
     2461 tccttgagat gcgcgaaacc ctcttcgatg cggttatgat cgtagtagaa ctgcgccagt
     2521 gagatgcgcg tcatgctgtc gtccgggtcc agttccaggg ctttcttgta cagcgcctcg
     2581 gccttggtgc tgtcgcccat ggagtcgtac acctcggcca gcatgccgta gtactgggtc
     2641 tgacccggct gctccttgat cgccttgtcc aagaggtcgc gggcggcggc cgtctcgccg
     2701 gcattggcga gcatgtcgta ctcgcgcatc accagttcct cgttgcttcc gatgcgcttc
     2761 tccaggtcgc ggaagacctg cctcgcctcg ccgatccgtt tctgctcggc cagtgtgttg
     2821 gccagcccga agtacacctc atagcgatcg ggccacttgg ccaggaggtc ctggtaggtt
     2881 ctggatgccc ccgccaggtc attctcctgc aactgcagat cggccaccag gaagcggtac
     2941 catatatttt ccttgtcggt ggccacggcg cgcttcgcca tggccatggc cggcgctgcg
     3001 gcctgggcct ggttgtacag cttgcccagt tcgaacatgc tggccgcatt ccgcggatcc
     3061 tccttgaggg tggcttcgta gagctggatg gccttgggca actcaccccg gagccgggcc
     3121 tgattggcct ccatgaagag ccgcatcacc aatgcgcgct tgctcttgaa ggaagcatcc
     3181 tgcgcggtcc cgaccccatc ggtcgcccgc ggcgcctcgg ccacgggttt tgcaccaccg
     3241 caggccagca gaacagccag caacggaacg aacagggt
//
LOCUS       DAOQOC010000198         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14643_length_3270_cov_2.902333, whole genome shotgun sequence.
ACCESSION   DAOQOC010000198 DAOQOC010000000
VERSION     DAOQOC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14643_length_3270_cov_2.902333"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PL106_04300"
     CDS             <1..997
                     /locus_tag="PL106_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA32322.1"
                     /translation="EKFGADGVRVGMLLTSPAGNDLPYDDSLCEQGRNFSNKIWNAFR
                     LVKGWSIDDRAQSASNTSACAWMRSRIERATKEIDDLYAQFRISEALMATYKLIWDDL
                     CSWYLESIKPAFVNGVGEPIDRTTYETTITLFEDVMRLLHPFMPFITEELWSHLRERK
                     GPQDALIIAAWPKGGAGDAKLEVEVQHAFDLVTAVRNTRNERGMSPKDTMELQVKGTS
                     PLSTSTIALVGKLANVSAINAVTTPADGSVTFLVGTTEYAVDLGGNVDAAAEAKKATE
                     ELNYLRGFLVSVDKKLSNERFVAGAPPQVLENERKKKADAEAKIKALEERLAVLR"
     gene            1334..1540
                     /locus_tag="PL106_04305"
     CDS             1334..1540
                     /locus_tag="PL106_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32323.1"
                     /translation="MNEIIFLVEESPEGGYEAKALGYSIFTDGDDLEQLKANVKDAVA
                     THFEAADQPKVIRLHMVKEIVIAA"
     gene            1537..1776
                     /locus_tag="PL106_04310"
     CDS             1537..1776
                     /locus_tag="PL106_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNA32324.1"
                     /translation="MRLPRTLSGKELIAALGKLGYTVTRQSGSHIRLTTDVNVIHHVT
                     IPDHRPLKVGTLSAILRDVAAHHGVDREELVRRLF"
     gene            complement(1832..2803)
                     /locus_tag="PL106_04315"
     CDS             complement(1832..2803)
                     /locus_tag="PL106_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNA32325.1"
                     /translation="MKARNILPLFGMVLLSSCLKEELPVPARTLGDVQVGQACVGSDY
                     GDQVWYDLGTNSIVSVNSKMDWDLAFECGADGWQVRVNTSRSMRALEKSGTDITQPLD
                     TNGYGPLWRIDHNCGSADSTAIRDWRTDHPVYAIDMGYSDIGLAMGVRQLRVTAVDAN
                     GYTFEVANANGTNVHTYTIAKDPQRTYVHFRISSGQVVTIAPPKGSYDLVFTQYTYQF
                     YEPVLAYLVTGVVNGFSGSRTARIITDDFASVSLTDTLAHPFTSDEDGVGYEWKDYDF
                     DLAVYTVFPERVYIIEDSEGQYFKLHFTDFYDENGTRGCPKFELVAL"
     gene            complement(2800..>3270)
                     /locus_tag="PL106_04320"
     CDS             complement(2800..>3270)
                     /locus_tag="PL106_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA32326.1"
                     /translation="MGGSAGIGWDNGHWLASLGGATTARHDDLGVDVGGAAWLVTPEL
                     RGSITREWRKQGWSASCFVKYQGEQGSYVYSSESVVGRNTLDPYVMADVNVAKRLFDK
                     RLRLAVGCKNIANVTNVGSTVAAGGVHGGGGGVVPMAIGRTWFLRIDVDLNRTE"
BASE COUNT          684 a         1018 c          971 g          597 t
ORIGIN      
        1 cgagaagttc ggtgccgacg gcgtgcgcgt gggcatgctc ctcacctccc ctgccggcaa
       61 cgacctgccc tacgacgatt cgctgtgcga gcagggccgc aacttcagca acaagatctg
      121 gaacgccttc cgcctcgtca agggctggag catcgatgat cgcgcacaga gcgccagcaa
      181 tacgtcggcc tgtgcctgga tgcgttcgcg tattgaacgc gcaacgaagg agatcgacga
      241 cctctacgcg caattcagga tcagtgaggc gttgatggcc acgtacaaac tcatctggga
      301 cgacctctgc agctggtacc tggaaagcat caaaccggcc ttcgtgaacg gcgtgggcga
      361 accgatcgac cggaccacgt atgaaacaac gatcaccctg ttcgaggatg tgatgcgcct
      421 gctccatccc ttcatgccct tcatcaccga agagctgtgg agccacctgc gcgaacgcaa
      481 gggcccgcag gacgcgctga tcatcgcggc atggccgaag ggcggtgctg gcgatgcgaa
      541 gctggaagtc gaggtgcagc acgccttcga cctggtgacc gccgtgcgca acacacgcaa
      601 cgagcgcggc atgagcccga aagacacgat ggagttgcag gtgaaaggca cgagcccgct
      661 cagcacatcg accatcgcgc tggtgggcaa gctcgccaac gtgagcgcga tcaacgcggt
      721 gaccacaccg gccgatggtt ccgtgacctt tcttgtgggc accacggaat acgccgtgga
      781 ccttggaggt aatgtggacg ccgccgctga ggccaaaaag gccacggaag agctgaacta
      841 cctgcgcgga ttcctggtga gtgtggacaa gaagctcagc aacgagcgct tcgtggccgg
      901 tgccccgccg caggtgctgg agaacgagcg caagaagaag gccgacgccg aggcgaagat
      961 caaggcgctg gaggaacggc tggctgtgct gaggtgaaga cttcagccac ccgttgcctt
     1021 gtatttttga agtccgggtt caaaaatgca aggcaaggaa tatcgtttca tcacctccgg
     1081 caccgagccc cgcaaactca cgcccggcat tgatgtagtg tcctggcgaa tgatcacttc
     1141 cggagagatc aacctgttct gatccgatca gcaacgaggt gcatccatgg ggaacacgcg
     1201 ctacgttgga tgatcgcaaa gcaacgccat gcgaagatca aggccctgga ggaaagaccg
     1261 gcggtgctgg gctgatcagc atggttgctt cgtccttggg ccaccagcta tctttacaga
     1321 cacaacaacg cgcatgaacg agatcatctt cctggtcgaa gaatcgccgg aaggcggata
     1381 tgaggccaag gcgctgggct attccatttt caccgatggc gatgacctgg agcagttgaa
     1441 ggcgaacgtg aaggacgccg tggccacaca tttcgaggcg gccgatcaac ccaaggtgat
     1501 ccggctgcac atggtgaagg agatcgtgat cgccgcatga ggttgcctcg cacgttgagc
     1561 gggaaggaac tgatcgccgc actgggcaag ctcggctata ccgtcacccg gcaaagcggc
     1621 agccatatcc ggttgaccac cgatgtgaac gtcatccacc acgtgaccat tcccgaccac
     1681 cgtccgctca aggtgggaac cttgtccgcg atccttcggg acgtggcggc acatcacggg
     1741 gtggatcgcg aagaattggt ccgacggttg ttctgagaag tgccaggcga agatcaaggc
     1801 gctggaggaa cggctggcgg tgccaggctg atcacaatgc caccaactcg aacttcgggc
     1861 acccacgcgt accgttctcg tcgtagaaat ccgtgaagtg gagcttgaag tactgccctt
     1921 cactgtcctc gatgatatag acgcgttcgg ggaacacggt gtacacggcc aggtcgaaat
     1981 cgtaatcctt ccactcatag cccacaccat cctcgtccga ggtgaacgga tgcgcgagcg
     2041 tatccgtgag cgatacgctc gcgaagtcgt ccgtgatgat ccgcgctgta cgggagccac
     2101 tgaaaccgtt caccacaccg gtgaccaggt aggccaggac cggctcgtag aactggtagg
     2161 tgtactgcgt gaagacgagg tcgtagctcc ccttcggcgg ggcgatggtg accacctggc
     2221 cgctgctgat cctgaaatgc acgtaggtac gctgcgggtc cttggcgatg gtgtacgtat
     2281 gcacgttggt gccattggcg ttcgccacct cgaaggtgta tccgttggca tccacggccg
     2341 tgacacgcaa ctgacgcacg cccatggcca ggccgatgtc gctgtacccc atgtcgatgg
     2401 cgtacaccgg atgatccgtg cgccaatcgc ggatcgccgt gctgtccgct gacccgcagt
     2461 tgtgatcgat gcgccaaagt ggcccgtagc cattggtgtc caaaggctgt gtgatgtccg
     2521 tgccgctctt ctccaacgcc cgcatggacc gggaggtgtt cacccgcacc tgccatccgt
     2581 ccgcaccgca ttcgaaggcg aggtcccagt ccatcttgct gttcacactg acgatggaat
     2641 tcgtaccgag atcgtaccac acctgatcac catagtcgct tccgacgcag gcctgtccca
     2701 cctgcacatc tccgagcgta cgcgctggta cggggagttc ctccttcagg cagctgctca
     2761 acagcaccat gccgaacaac gggaggatgt ttcgcgcctt cattccgtcc tgttcagatc
     2821 gacatcgatg cgcaggaacc atgtgcgccc gatggccatg ggtaccacgc cgcctccgcc
     2881 gccatgcaca ccacccgccg ccaccgtgga tcccacgttg gtcacgttcg cgatgttctt
     2941 gcaacccacg gccaggcgca gccgtttgtc gaagagtcgc ttcgccacgt tcacatcggc
     3001 catcacgtac ggatcgagtg tattgcgccc caccaccgat tccgatgagt agacatagct
     3061 gccctgttcg ccctggtact tcacgaagca ggacgcgctc cagccctgct tgcgccattc
     3121 ccgcgtgatc gatccgcgca gctccggcgt aaccaaccac gctgcaccgc ccacatccac
     3181 gcccaggtca tcgtgtcgcg cggtggtggc accaccgagg ctcgcaagcc agtggccatt
     3241 gtcccaaccg atcccggcgc tgccacccat
//
LOCUS       DAOQOC010000199         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14648_length_3269_cov_5.001556, whole genome shotgun sequence.
ACCESSION   DAOQOC010000199 DAOQOC010000000
VERSION     DAOQOC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14648_length_3269_cov_5.001556"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="lpxB"
                     /locus_tag="PL106_04325"
     CDS             complement(<1..974)
                     /gene="lpxB"
                     /locus_tag="PL106_04325"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNA32327.1"
                     /translation="MARLYFIAGEASGDLHGSNLIAELFKKGIEWRVASGEAVDDTAQ
                     PLAIRHTQLVIRAWGGDRMAAAGAEVVKHYRELAFMGFTQVVMNLRTILRNIRLCKED
                     IAAFKPDALVLIDYPGFNLRIADWARTQGIQVFYYISPQLWAWKENRIAVVKRAVDRM
                     FCILPFEKDWYAQRGVDVDFVGHPLLDELAKHADPVEKDTRDAVAILPGSRTQEITRM
                     LPVMLDAAKHFPTERFVVAAAPSVPEAFYRQLIGDAHVELVAGRTYDLLREAKAAIVT
                     SGTATLETALFGVPEVVCYSGSSINVWLARRLVKVRVISLVNLIMDREA"
     gene            complement(975..1268)
                     /locus_tag="PL106_04330"
     CDS             complement(975..1268)
                     /locus_tag="PL106_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32328.1"
                     /translation="MDSLAIGIILGLLGPVAGFFAYGTIYVTAIKSQYDLHWFVNDLF
                     IGTPPLRARVLSLSLIADALIFFALDRFHLYKSMRGVIAAMLAYAVVVIIFLF"
     gene            complement(1288..2064)
                     /gene="surE"
                     /locus_tag="PL106_04335"
     CDS             complement(1288..2064)
                     /gene="surE"
                     /locus_tag="PL106_04335"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNA32329.1"
                     /translation="MTNKPLILVTNDDGISAPGIHVLAQEMKAFGRVLVVAPDKPQSA
                     MGHAITIHDHLRLNKVEMMEGVEAWSCSGTPVDCVKLAVYRLLKGERPAVLVSGVNHG
                     SNISINVLYSGTMSAAVEGAMEGIQSVGFSLMDHSIEARFQHVRPIIRGVVANVLEKG
                     LARGTCLNVNIPRESDGPLKGVRVCRQAWGYWEDDFEARTDPMGKEYYWLRGRFKDMD
                     QGEDTDVWAVNNGYVSVVPVQFDMTAHHTIADLNTWDHAR"
     gene            2146..2577
                     /locus_tag="PL106_04340"
     CDS             2146..2577
                     /locus_tag="PL106_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNA32330.1"
                     /translation="MLNFARPWQMRTWARMHMNRKPIHPPGAAKPLAPYTPAIEHNGV
                     IYLSGQIALRGDTGELVMDTIEAETTQVMENIGLLLRAAGLGYDDLLKVTILLSDMSH
                     YGAVNTVYGTYFKDAPPAREAFAVKGLPRGVNVEISGIAGR"
     gene            complement(2588..2806)
                     /locus_tag="PL106_04345"
     CDS             complement(2588..2806)
                     /locus_tag="PL106_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32331.1"
                     /translation="MGVPIAENRWRLKGDGFHIYTTAQLLGILLPMLVVGTELFNFLG
                     LVIAALWCVMYYAQMRAIGALGSGLGRS"
BASE COUNT          637 a         1003 c         1025 g          604 t
ORIGIN      
        1 gcctcccggt ccatgatcag gttcaccagg gagatgaccc gcaccttcac cagcctgcgc
       61 gcgagccaca cgttgatgga gctgccgctg tagcagacca cttcgggcac accgaacagg
      121 gcggtctcca gggtggccgt gccactggtc acgatggcgg ccttggcctc gcgcaagagg
      181 tcgtatgttc ttccggcgac gagttccacg tgcgcatcgc cgatcaactg ccggtagaag
      241 gcctcgggca ccgatggcgc ggccgccacc acgaagcgtt cggtgggaaa gtgtttcgcg
      301 gcatcgagca tcacgggcag catgcgggtg atctcttgcg tgcgactgcc ggggaggatc
      361 gctacggcat cacgtgtatc tttttcgacg ggatcggcat gcttcgccag ttcatccaac
      421 agcgggtggc ccacgaagtc cacgtccacc ccgcgctggg cataccagtc cttctcgaat
      481 ggaaggatgc agaacatgcg gtccacggcg cgcttcacaa cggcgatccg gttctccttc
      541 caggcccaga gctgcgggct gatgtaatag aacacctgga tgccctgcgt cctcgcccaa
      601 tcggcgatgc ggaggttgaa gccggggtag tcgataagca caagggcatc gggcttgaat
      661 gcggcgatgt cctctttgca cagccggatg ttgcgcagga tggtgcgcag attcatcacc
      721 acttgggtga aacccatgaa ggcgagctca cggtagtgct tcaccacttc ggcaccggcc
      781 gcggccatgc gatcaccgcc ccaggcacgg ataacgagtt gtgtgtggcg aatggcgagt
      841 ggctgggctg tatcgtcgac agcctcgcca ctcgccactc gccactcgat accctttttg
      901 aacagttccg cgatcaggtt ggatccgtgc aggtcgccac tggcctctcc cgctatgaag
      961 taaaggcggg ccattcagaa gaggaagatg atgacgacca cggcgtatgc cagcatggcc
     1021 gcgatcaccc cgcgcatgct cttgtacagg tggaagcggt ccaatgcgaa aaagatgagc
     1081 gcatcggcga tgaggctgag ggaaagcacc cgggcgcgca gggggggcgt gccgatgaag
     1141 aggtcgttga cgaaccagtg caggtcgtac tgggacttga tggcggtaac gtagatggtg
     1201 ccgtacgcga agaatcccgc cacggggccg agcaggccaa gaatgatccc gatagcgagg
     1261 gaatccatcg gatgcgatga ccggtgctca ccgcgcatgg tcccaggtgt tcaggtcggc
     1321 gatggtgtgg tgcgcggtca tatcgaactg tacgggcacc acactcacgt agccgttgtt
     1381 cacggcccac acgtccgtgt cctcaccctg gtccatgtcc ttgaaccggc cgcgtagcca
     1441 gtagtattcc ttgcccatcg ggtcggtgcg cgcctcgaag tcgtcctccc agtagcccca
     1501 ggcctggcgg cacacgcgca cacccttcag cggcccgtca ctctcgcgcg ggatgttcac
     1561 gttcaggcag gtgccccggg ccaacccctt ctccaggaca ttggccacca cgccgcggat
     1621 gatcggtcgc acgtgctgga atcgggcctc gatgctgtgg tccatgagtg agaagccgac
     1681 gctctggata ccttccatcg caccttccac cgcggcgctc atggtgccgc tgtatagtac
     1741 gttgatgctg atgttgctgc cgtggttcac accgctcacg aggacggccg gcctctcgcc
     1801 cttgagcaag cggtacacgg ccagcttcac gcaatccacg ggtgtgccac tgcacgacca
     1861 cgcctccaca ccctccatca tctccacctt gttcagtcgg agatggtcgt gtatggtgat
     1921 cgcgtggccc atggcgcttt gtggtttgtc cggtgccacc acgagcacgc gcccgaaggc
     1981 cttcatttcc tgggcaagta catggatccc cggcgcggag atgccgtcgt cgttcgtgac
     2041 gaggatgagg ggcttgttgg tcatcagacc gcgaaactat cccatggacc catgtgcgct
     2101 cgcggccagc gccccgtgcg tccgtgtgcc catgatgatc cgacaatgct gaacttcgcg
     2161 cgcccatggc aaatgcgcac atgggcccgg atgcacatga acaggaagcc gatccatcct
     2221 cccggtgccg cgaagccgct ggcaccgtat acgccagcca tcgagcacaa cggcgtgatc
     2281 tacctcagtg gccagatcgc cttgcgcggc gataccggcg aactggtcat ggacaccatc
     2341 gaagcggaaa ccacccaggt gatggagaac atcggcttgc ttctgcgtgc tgccggactg
     2401 ggctacgacg atctgctcaa ggtgacgatc ctgctgagcg acatgtcgca ctatggagcg
     2461 gtgaacaccg tgtacggcac gtacttcaag gatgcgcctc ccgcgcgcga ggcgttcgcc
     2521 gtgaagggct tgccacgtgg ggtgaacgtg gagatcagcg ggatcgcggg gagataggcc
     2581 ggctcggtca actcctgccc aaaccactgc ccaaggcacc gatggccctc atctgggcgt
     2641 agtacatcac gcaccagagc gcggcgatca ccaggccgag gaagttgaac agttccgtac
     2701 cgaccacgag catgggcagc aggataccga gcagttgcgc cgtagtgtag atgtggaagc
     2761 cgtcgccctt cagccgccac ctgttttcag cgatcgggac acccaagatg atcgctgtgg
     2821 gcaagtgttt gtggtcaaga tccggaacga gcgctgagag cgggagtatt tcgcttcggc
     2881 gggctcagcg tgacagtggg gagatgagta tccaagagcg ctgttcttga acactcccga
     2941 agagctgtca cgctgagccc gccgaagcgc tacaactagc cggtattgtt cctgaatacg
     3001 cgatcctcag cacattcaga ccggtctgaa cacttctttg cgcgggaatg gggacagagg
     3061 gcgggtcacc cgatcaaatt cagatgtgcc cggccatcaa ggcttgcggc gtacgatccc
     3121 gttggaattc ggggtgatcg ctgaaaacag gaacgtggag atcagcggga tcgcggggcg
     3181 gtagtagaga ccgggtcagc ccctgcccaa gccgctgccc aaggcaccga tggccctcat
     3241 ctgggcgtag tacatcacgc accagagcg
//
LOCUS       DAOQOC010000200         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14675_length_3265_cov_3.659502, whole genome shotgun sequence.
ACCESSION   DAOQOC010000200 DAOQOC010000000
VERSION     DAOQOC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14675_length_3265_cov_3.659502"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PL106_04350"
     CDS             <1..981
                     /locus_tag="PL106_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNA32332.1"
                     /translation="IRKRLLEYDDVMNRQRQVIYKRRHNALFGERLKLDVLNMFHDTA
                     TDLVEQYHDEGDFEGFRMDLFRKLAIESAVDEAGFKSGKTPDIAERVFRSAMDAYQRR
                     GSQTATQALPVISDVFLNQGAQYENIVVPITDGTKTMQVVTNLQRAYKSECRELIDSI
                     ERYITLAIIDDEWKEHLREMDDLRRNVQNASIEQKDPLLIYKLESFKLFDAMIARISN
                     EVTGFLVRAGLPTAQPNVQQAQAPRPPQQRLQTSRTESPQFAGSSSSAAPAPQQRMAA
                     PAGRGPVPPHPGGPPRPPVMPVRVEKKVGRNDPCPCGSGRKFKQCHGKDA"
     gene            complement(1057..1713)
                     /locus_tag="PL106_04355"
     CDS             complement(1057..1713)
                     /locus_tag="PL106_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNA32333.1"
                     /translation="MLALLFSTMLCGPIPGPMGKGPERTWLNSVLEPTTKAKAVYYRI
                     PTGRQGDAYLGKILTLDDVLKAEGQYADPALTIPHGHFVFYYGDGGKESEGDYAMGNK
                     TGVWQRFDKWGTALAEKVYDLKVLEDLVYTTAPTMPQYPGGEHAMITYLKDKAGRVSG
                     ATASFVVEKDGELSDIKVLGASQDVADQVADALKQAPRFTAGEKDGLPVRVQMRVPLK
                     "
     gene            complement(1803..>3265)
                     /locus_tag="PL106_04360"
     CDS             complement(1803..>3265)
                     /locus_tag="PL106_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNA32334.1"
                     /translation="GTNFFMVGLPDNAVKESHQRVSAAIKNIGLHMPRGREITVNLAP
                     ADIRKEGTAYDLPIALGIIAASEQLDPARLSEYAIMGELALDGEVRPIKGTLPIAIEA
                     KKQGFKGLIVPSANAREAAIVEGLEVIGVAHLRQVVDFLEKREAIAPTEVDIRAEFDQ
                     SSRSYPVDLSDVKGQENIKRAFEIAAAGGHNVILIGPPGAGKTMIAKRLPTILPPLNI
                     EEALETTKIHSVAGKLRKDDSLLSVRPFRSPHHTISDVALVGGGSFPQPGEISLAHNG
                     VLFLDELPEFQRTVLEVMRQPLEDRVVTISRAKFTVEYPASFMLVAAMNPCPCGYYNH
                     PEKECVCAPGTVQKYLNRISGPLLDRIDIHIEVTPVAFSELSSERVTEKSIDVRERVI
                     AARHVQEQRYAGTKMYCNAQMSSQQLRSICRIDAAGQALLQRAMERLGLSARAYDRIL
                     KVARTIADLAHSPDIRTEHLAEAIQYRSLDREGWAG"
BASE COUNT          567 a         1032 c         1031 g          635 t
ORIGIN      
        1 atccggaagc gcctgctgga gtacgacgac gtgatgaacc ggcagcggca ggtgatctac
       61 aagcgccgcc acaacgccct tttcggcgaa cgcctgaaac tggacgtgct caacatgttc
      121 cacgatacgg ccaccgacct ggtggagcag taccacgacg agggggactt cgaaggcttc
      181 cggatggacc tgttccgcaa gctggccatc gagagcgccg tggatgaggc gggcttcaag
      241 agcggcaaaa cgcccgacat cgccgagcgg gtcttccggt cggccatgga tgcctaccag
      301 cggcgcggta gccagacggc gacccaggcc ctgcctgtga tcagcgatgt gttcctgaac
      361 cagggtgcgc agtacgagaa catcgtggtg cccatcaccg atggtaccaa gaccatgcag
      421 gtggtcacca acctacaacg ggcgtataag agcgaatgcc gcgagctcat cgacagcatc
      481 gaacggtaca tcaccctggc catcatcgac gacgagtgga aggagcacct gcgcgaaatg
      541 gacgacctgc gccgcaacgt gcagaacgcg agcatagagc agaaggaccc gctcctcata
      601 tacaagctgg agtcgttcaa gttgttcgat gccatgatcg cgcgcatcag caatgaggtc
      661 accggcttcc ttgtacgtgc cgggttgcca actgcgcaac ccaacgtgca gcaggcacag
      721 gcaccacgcc cgccgcaaca gcgcttgcag accagtcgca ccgagtcccc gcaattcgcg
      781 ggcagcagca gcagtgctgc accggctccg caacaacgca tggccgctcc tgctggtcgt
      841 ggtccggttc ccccgcatcc cggtggtcca cctcgcccgc ccgtgatgcc cgtacgtgtg
      901 gagaagaagg tgggccggaa cgacccctgt ccgtgcggca gcgggcggaa gttcaaacag
      961 tgccacggga aggacgcatg aaccttcgcc ccatgacgaa cacggacgtc ggccttacgg
     1021 ggccgacgtt catgttatgg tgtatgtcgt gtggttctat ttcaggggca cgcgcatctg
     1081 cacgcgcacg ggcagtccgt ccttctcgcc cgctgtgaat cgcggggcct gtttcagggc
     1141 gtcggccact tggtcggcca catcctgtga tgcgcccagc actttgatgt cgctcagttc
     1201 gccatccttc tccaccacga aggaggccgt ggcaccgctc acgcggcccg ccttatcctt
     1261 cagataggtg atcatcgcat gttcaccccc gggatattgg ggcatggtgg gcgctgtggt
     1321 gtacacgaga tcctccagca ccttcaggtc atagaccttt tcggccaggg ccgtacccca
     1381 tttgtcgaat cgttgccaca caccggtctt gttccccatg gcgtagtcgc cctcgctttc
     1441 cttgccaccg tcaccgtagt agaacacgaa gtgaccatga gggatcgtca atgcggggtc
     1501 ggcgtattgg ccttcggcct tcagcacatc gtccaaggtg aggatcttgc ccagataggc
     1561 atcgccctgg cgccctgtgg ggatacgata gtacacggcc ttcgccttgg tggtgggttc
     1621 cagcacgctg ttcaaccagg tgcgctccgg acctttgccc atggggccgg ggatcggacc
     1681 gcagagcatg gtggagaata gaagcgcgag catcggggtt gatcggaagt gttggtgtac
     1741 aacgcgacaa ccacccggaa ggtttcaccg ctccgcagtg ggacaggtca tgcgcttcct
     1801 gttcagcccg cccacccctc gcgatcgagg ctgcggtact ggatggcctc ggccaggtgc
     1861 tcggtccgga tgtccgggct gtgggccagg tcggcgatgg tgcgcgccac cttcaggatg
     1921 cggtcgtagg cccgggcact gaggccgagg cgttccatgg ccctctgcaa caaagcctgt
     1981 ccggcggcat cgatccggca gatgctccgt agctgctggc tgctcatttg cgcgttgcag
     2041 tacatttttg ttccggcgta gcgctgttcc tggacatggc gggccgcgat cacacgctcc
     2101 cgaacgtcga tgctcttctc cgtcacccgt tcgctgctca gttcactgaa ggccaccggc
     2161 gtcacctcga tgtggatgtc gatgcgatcg agcagcgggc cgctgatccg gttcaggtat
     2221 ttctgcaccg taccgggcgc gcacacgcac tccttctccg ggtgattgta gtagccgcag
     2281 gggcaggggt tcatggcggc caccagcatg aagctcgcgg ggtactccac ggtgaacttg
     2341 gcgcggctga tcgttaccac gcggtcctcg aggggctggc gcatcacctc aagcaccgtg
     2401 cgctggaatt cgggcagctc gtccaggaag agcacgccgt tgtgggccaa gctgatctcg
     2461 ccgggttgcg ggaatgagcc accgcccacg agtgccacgt cgctgatggt gtgatgcggc
     2521 gagcggaagg gccgcacgct caggagcgag tcgtccttgc gcaatttacc ggccacactg
     2581 tggatcttgg tggtctccaa tgcctcctcg atgttcagag gcggcaggat ggtgggcagc
     2641 cgtttcgcga tcatggtctt gcccgctccc gggggtccga tcaggatcac gttgtgcccg
     2701 cccgctgcgg cgatctcgaa cgcccgtttg atgttctcct ggcccttcac gtcgctcagg
     2761 tccaccgggt agctccggct gctctgatcg aactcggcgc ggatgtccac ttcggtcggc
     2821 gcgatggcct cgcgtttttc caggaaatcg accacttgcc ggagatgcgc gacgccgatg
     2881 acctccagtc cttcaacaat ggcggcttcg cgtgcgttgg cggacggtac gatgaggccc
     2941 ttgaaaccct gcttcttcgc ttcaatggcg atgggcaggg tgcctttgat ggggcgcacc
     3001 tcgccgtcca gggccagttc gcccatgatc gcgtattccg aaaggcgcgc gggatccaac
     3061 tgctcgctgg ccgcgatgat cccgagtgcg atgggcaggt cgtaggcggt gccctccttg
     3121 cggatatcgg ccggggccag gttcacagtg atctcacgcc cgcgcggcat gtgcagcccg
     3181 atgttcttga tggccgcgct cacgcgctgg tggctctcct tcaccgcgtt gtccggcagg
     3241 cccaccatga agaagttggt gccct
//
LOCUS       DAOQOC010000201         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14804_length_3252_cov_3.376916, whole genome shotgun sequence.
ACCESSION   DAOQOC010000201 DAOQOC010000000
VERSION     DAOQOC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14804_length_3252_cov_3.376916"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PL106_04365"
     CDS             complement(<1..1663)
                     /locus_tag="PL106_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNA32335.1"
                     /translation="MAEKHGRNKNNGRTPRSTGLRWWWVLVLTPFIGLALMLAIAATS
                     DDLPSTTELQNPRSDLATAVLFSDGSLMGQYYRENRIPVDYDRISPNVVNALIATEDE
                     RFREHSGIDVRSTLRALVFLGKKGGASTITQQLAKMLFHEPARGFFSRVRQKSQEWLI
                     SAKLEREYTKDEIIAMYLNRFDWINQAVGINSAARIYFNTTPDSLAVHQAALLVGMCK
                     NPALFNPLRRPDTTLTRRNVVLYQMEKNGFITGAQRDSLKALPLSLDFQRVDHTEGPA
                     PYLREVLREELKSLLAAKDPKTGALRIAKADGTPYDIYTDGLRIYTTIDPRMQRYAEF
                     AVREHLGTELQPQFFKDIKDKKNRPFDFRVTKEEVASIMDAAMKRSDRYKTYTGKLCA
                     NCQRPAAYITTMVTDGKKYYHCDPAKGGCDTKWPVRSEKEIVAAFDQKEKMRVFSWKG
                     EIDTLFSPNDSIRYYKSFLLSGLLSLDPATGFVKAWVGGPDFKHFQYDHVQQAHRQVG
                     STFKPFIYATAIREGMDPCLRVPNQKTCFDMPEGQPDWCPENSDAK"
     gene            complement(1747..2226)
                     /locus_tag="PL106_04370"
     CDS             complement(1747..2226)
                     /locus_tag="PL106_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNA32336.1"
                     /translation="MKYPIVPVLLCLLLATGCTDGVVFQEDARTPHGAWERGWDPSFS
                     FDMRDTTNPHDVYLDLRHTGDYPFSNLYAFVTLTGPDSVPVTDTVECLLADPTGRWYG
                     SGTGFIRSHRQAHVLYKLRNKFPRSGRYTITLRQAMRTEKLDGVTDVGVSVERSKGG"
     gene            complement(2223..>3252)
                     /gene="ricT"
                     /locus_tag="PL106_04375"
     CDS             complement(2223..>3252)
                     /gene="ricT"
                     /locus_tag="PL106_04375"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNA32337.1"
                     /translation="GDGTKATFYYTSEQRVDFRNILRPLSDRFKVKVEMKQIGARQET
                     GRIGGIGSCGRELCCSTWLTDFRSVTTSAARYQQLALNPQKLAGQCGKLKCCLNYELD
                     MYIEAVKSYPSQNAKLKTRQGTGYHMKTDIFEEKMWYLFKMPGSAPVMAGFPVEKVRE
                     ILAQMKEGIEVDVDFAALAEENEAAKNAGVEAEPEYESVGGEDELTRFDSKIKSGKRN
                     KRGGKGRDRDRRGGGGDRPQGQAQQASRSGGNGQGPRPQQGPRPPRPDRPQGQRPSRP
                     EQPRTDRPAAPERQAQPDRNDGGRPQGDRPPTEGGRNRHRRHRDRRGSRSEGQQGGGG
                     APDTPPAA"
BASE COUNT          482 a         1026 c         1056 g          688 t
ORIGIN      
        1 acttggcatc gctgttctcg gggcaccagt cgggctggcc ctccggcatg tcgaagcagg
       61 tcttctggtt gggtacccgc aggcagggat ccatgccctc gcggatggcg gtggcgtata
      121 tgaacggctt gaaggtgctg cccacctggc ggtgcgcctg ctgcacgtgg tcgtactgga
      181 agtgcttgaa gtcaggcccg cccacccagg ccttcacgaa gccggtggcc gggtcaaggc
      241 tcagcagccc gctcaacagg aagctcttgt aataccggat actgtcgttg gggctgaaga
      301 gcgtgtcgat ctcgcccttc cagctgaata cgcgcatctt ctccttctga tcgaacgcgg
      361 cgacgatctc cttctccgac ctcacgggcc atttcgtgtc gcagccaccc ttggcagggt
      421 cgcagtgata gtatttttta ccgtcggtca ccatggtggt gatgtacgcg gccggccgct
      481 ggcagttggc gcagagcttg ccggtgtacg tcttgtaccg gtcactgcgc ttcatggcgg
      541 cgtccatgat gctcgcgacc tcctccttgg tcacgcggaa gtcgaatggc cggttcttct
      601 tgtccttgat gtccttgaag aactggggct ggagttccgt gccgaggtgc tcgcgtaccg
      661 cgaactcggc atagcgctgc atgcgcgggt cgatcgtggt gtagatccgc aggccatcgg
      721 tatagatatc gtatggtgta ccatccgcct tggcgatgcg gagcgctccg gtcttcgggt
      781 ccttggccgc cagcaggctc ttcaactcct cacggagcac ctcgcgcaag taaggtgccg
      841 gtccttcggt atgatccacc cgctggaagt caaggctgag cggaagggcc ttgagcgaat
      901 cgcgctgggc accggtgatg aagccgttct tctccatctg gtagagcacc acattgcggc
      961 gtgtcagcgt ggtatccggc cgccggagcg gattgaagag cgcgggattc ttgcacatgc
     1021 ccacgagcag ggcggcctgg tggaccgcga gggagtcggg ggtggtgttg aagtagatgc
     1081 gcgcggcact gttgatcccc accgcctggt tgatccagtc gaagcggttg aggtacatgg
     1141 cgatgatctc gtccttggtg tactcgcgtt cgagtttggc gctgatgagc cattcttgcg
     1201 acttctgccg cacccgggag aagaagccgc gcgccggttc atggaagagc atcttggcga
     1261 gctgctgggt gatggtgctg gcaccaccct tcttccccag gaaaacgagc gcgcgcaggg
     1321 tgcttcgcac atcaataccg ctgtgttccc ggaagcgttc atcctccgtg gcgatgaggg
     1381 cgttcaccac gttggggctg atgcggtcat aatccactgg gatcctgttc tcacggtagt
     1441 attgccccat gagactgcca tcgctgaaga gcacggcggt ggccaggtcg ctgcgcgggt
     1501 tctgcaattc ggtggtgctg ggcaggtcgt cgctggtggc ggcgatcgcc agcatgagcg
     1561 ccagtccgat gaagggagtg agcacgagca cccaccacca ccggagcccc gtggatcgcg
     1621 gtgtgcggcc gttgttcttg ttcctcccgt gcttttccgc catggtccaa agatggttgc
     1681 cgcgttcaac gatcgccgca ccgcgcaggg cgggatcctc accgggctat caacaacggg
     1741 gcccgctcag ccgcccttgc tgcgttccac gctgacgccc acgtcggtca ccccatcgag
     1801 cttctcggtg cgcatggctt gtcgcagggt gatggtataa cgcccgctgc gggggaactt
     1861 gttgcggagc ttgtacagaa catgggcctg ccggtgactg cggatgaagc cggtgcccga
     1921 accgtaccag cggccggtgg ggtcggccag caggcactca acggtatcgg tgacaggtac
     1981 actgtccggc ccggtaaggg tgacaaaggc gtacaggttg ctgaacggat agtcgccggt
     2041 gtggcgcaga tccaggtaca catcgtgcgg gttggtcgtg tcccgcatgt cgaaactgaa
     2101 actcgggtcc cacccgcgct cccaggcacc atgcggcgtc ctggcgtcct cttggaacac
     2161 cacgccatcg gtgcagcccg ttgccaacag caggcacagc aggaccggta cgatcggata
     2221 cttcaagcgg ccggcggtgt gtcaggtgcg ccgccccccc cctgctgacc ttcggaacga
     2281 gaaccgcggc gatcacggtg gcggcggtgc ctgttgcggc ctccctctgt aggtgggcga
     2341 tcaccctgtg gccgtccccc gtcattccgg tcgggttgcg cctgtcgctc cggtgcggcc
     2401 ggtcggtcgg tccggggttg ctcaggacgc gagggccgtt gtccctgcgg ccggtccggt
     2461 cgtggtggcc tcggcccctg ctgaggtcgg gggccctgtc cgttgccacc gctcctggat
     2521 gcctgctggg cctgtccttg tggacggtct cctccgccac cgcgccgatc gcggtcgcgg
     2581 cctttgccgc cgcgtttgtt ccgtttgccg ctcttgatct tgctgtcgaa ccgcgtcagc
     2641 tcatcctcac cacccacgct ctcgtactcg ggttcggcct caaccccggc gttcttggcg
     2701 gcctcgttct cctcggccag cgccgcgaag tccacgtcca cctcgatgcc ctccttcatc
     2761 tgtgccagga tctcgcgcac cttctcgacc gggaaaccgg ccatcacggg cgcactgccg
     2821 ggcatcttga aaagatacca catcttctcc tcgaagatgt cggtcttcat gtggtagccc
     2881 gtgccctgcc gggtcttcag tttggcgttc tgcgaggggt agcttttcac cgcctcgatg
     2941 tacatgtcca gctcgtagtt caagcagcac ttcaacttgc cgcactggcc ggcgagcttc
     3001 tgcgggttca gcgcgagctg ctggtagcgc gcggcgctgg tggtcacgct gcggaagtcg
     3061 gtgagccagg tgctgcagca cagctcgcgg ccgcagctgc cgatgccgcc gatgcgaccg
     3121 gtctcctgcc gcgcgccgat ctgcttcatc tccaccttca ccttgaagcg gtcactgagc
     3181 ggacgcagga tgttccggaa gtccacgcgc tgctcgctgg tgtagtagaa ggtggccttg
     3241 gtgccgtcgc cc
//
LOCUS       DAOQOC010000202         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14813_length_3251_cov_2.863267, whole genome shotgun sequence.
ACCESSION   DAOQOC010000202 DAOQOC010000000
VERSION     DAOQOC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14813_length_3251_cov_2.863267"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PL106_04380"
     CDS             <1..475
                     /locus_tag="PL106_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNA32338.1"
                     /translation="EQRIVTEAVMAALRSVGAVHVMARDPETLLAGEVAHLRTRVEAD
                     LGAVPLSDLVLALHPTPAVCGAPREAAREFIRNTEEHDRGLYTGFWGPWSADGRTELY
                     VNIRCLRHGVDHALIYAGAGITAGSDPRLEWEETEQKTRIWARPVADVDGTGSQA"
     gene            534..2270
                     /gene="menD"
                     /locus_tag="PL106_04385"
     CDS             534..2270
                     /gene="menD"
                     /locus_tag="PL106_04385"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNA32339.1"
                     /translation="MSSDHFSAVALAHLCAAKGVKHAVISPGSRDAPLIIAFNAQQGM
                     ECLRVIDERSAAFFALGMAQQLHAPVALICTSGSAVLNYAPAVAEAFYQRVPLLVICA
                     DRPEEWIDQGEGQTIRQRDVLVPHVKKSVQLPRAAVDDLSRWHCGRLINEAIDATLLP
                     VPGPVQVNVPFEEPLYGRTGNGGEDLFQQASTPMMERPRAISRVLTEPFILPGHARWL
                     VQQLSSSMRVMILAGQGVWTEGLKAQLLALAGLPQITVFTEATSDLDDPAFITTIDRT
                     IAGVRPENENGLRPELLITFGGAIVSKRIKDLLRAWKPGQHWNVDPGQRHVDTYQCLS
                     HDIAVSPETFFAQITAPVVAASAGRTDSFYREAWKMTEERMRGAHQHILASTSFCDLT
                     VFNTLVDRIPADSDVHLASSTPARYAQLFDRTRGLRFFANRGTSGIDGCTSTAVGAAH
                     VSGRLTTLITGDTAFCYDSNAFWNGYLSPHLKVIVIDNGGGNIFRFIEGPDRDPELLP
                     WFEAPHGRDIARLVKSFGLYHIHAEDAASLAAGLDELYKDHGRPAVLHVTTDARTSPK
                     VLRGYFKQLREA"
     gene            2309..3124
                     /gene="menB"
                     /locus_tag="PL106_04390"
     CDS             2309..3124
                     /gene="menB"
                     /locus_tag="PL106_04390"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNA32340.1"
                     /translation="MRNWTTIKEYADIRFDLFDGIARITINRPEVYNAFRPETNMEMI
                     DAMDIAREDTRIGVVVLTGAGDKAFCSGGDQNAKGRGGYIGSDGVPRLNVLDLHKRIR
                     ELPKPVVAMVNGYAIGGGHVLHVVCDLTIAADHARFGQTGPKVGSFDAGFGSNYLARH
                     VGQKKAREIWFLCLQYSAQEALDMGMVNKVVPLAELEDTTVEWCRIMMQRSPLALRMI
                     KRGLNAELDGQRGLMEFAGDATLMYYLMDEAQEGRNAFLEKRPPDFQKFPKFP"
BASE COUNT          601 a         1040 c         1062 g          548 t
ORIGIN      
        1 cgagcagcgc atcgtgaccg aggcggtcat ggccgcgctc cggagcgtcg gcgcggtgca
       61 cgtgatggcg cgcgaccccg aaacgctgct ggccggcgag gtggcccacc tgcgcacacg
      121 ggtggaggcc gatctcggtg ccgtcccttt gagcgacctg gtgctcgcat tgcaccccac
      181 gcccgccgtc tgcggcgcac cccgcgaggc ggcacgtgaa ttcatacgca ataccgagga
      241 gcatgatcgc ggcctgtaca cgggcttctg ggggccttgg agcgccgatg gccgcaccga
      301 attgtacgtg aacatccgct gtctgcgaca tggtgtggac catgcgctca tttatgcggg
      361 cgcggggatc accgcggggt ccgatccacg gctggagtgg gaggagaccg agcagaaaac
      421 gcgcatctgg gccaggcctg ttgcggacgt ggacgggaca ggctctcagg catgatcggc
      481 gaggagggtc gttgtgtgcg atgtccggtg tggcccggta gattcgccgc ccgatgagca
      541 gtgaccattt ctccgccgtg gccctggcgc atttgtgcgc tgccaaaggg gtgaagcatg
      601 cggtgatcag tcccggcagc cgggatgcac cgctcatcat cgccttcaac gcccaacaag
      661 gcatggagtg cctgcgcgtg atcgatgagc gcagcgccgc attcttcgcg ctgggcatgg
      721 cacaacagct gcacgcgccc gtggcgctca tctgcaccag cggcagcgcg gtgctcaact
      781 acgcaccggc cgtggccgaa gcgttctacc aacgcgtgcc actgctcgtc atctgcgccg
      841 accggcccga ggagtggatc gaccagggcg agggtcagac catccgccag cgcgatgtgc
      901 tggtcccgca tgtgaagaag agcgttcaat tgccacgcgc tgcggtcgat gatctttcgc
      961 gctggcactg cggtcggctc atcaacgagg ccatcgatgc caccctgttg cccgtgcccg
     1021 gtccggtgca ggtgaacgtg cctttcgagg agccgctgta cggccgtacc ggaaatgggg
     1081 gagaagacct cttccagcag gcctccacgc cgatgatgga acggccacgg gccatttcgc
     1141 gggtgctcac ggagccgttc atacttcccg ggcacgcgcg atggctcgtt cagcagcttt
     1201 cctccagcat gcgggtcatg atcctggccg gtcaaggcgt ttggaccgaa gggctgaaag
     1261 cgcagttgct cgcactggcc gggcttccgc agatcacggt gttcaccgag gccaccagcg
     1321 atctggacga tcccgcattc atcaccacga tcgaccggac cattgcgggc gttcgaccgg
     1381 agaacgagaa cgggctacga cccgaattgc tcatcacctt cggcggggcc atcgtgagca
     1441 agcggatcaa ggatctgctg cgcgcttgga aacccggtca gcactggaac gtggacccgg
     1501 gccagcggca tgtcgacacc tatcaatgcc tttcgcacga catcgccgtc agccccgaga
     1561 ccttcttcgc ccagatcacc gcgccggtcg ttgcggcatc ggccggccgc accgacagct
     1621 tttaccggga agcgtggaag atgaccgagg agcgcatgcg tggggcccac cagcacatcc
     1681 tcgcatccac gtcgttctgc gacctcaccg tgttcaacac gcttgtcgat cgcatacctg
     1741 cggacagtga tgttcatctg gccagcagca ccccggcgcg ttatgcgcaa ctgttcgacc
     1801 ggacgcgggg cctgcgattc ttcgccaacc ggggcaccag cggcatcgac ggctgcacga
     1861 gcacggccgt gggcgcggcg catgtttcag gcaggctcac cacactcatc accggcgaca
     1921 ccgccttctg ctacgacagc aacgcttttt ggaacgggta cctgtcgccg cacctgaagg
     1981 tgatcgtgat cgacaacggc ggcggcaaca tcttccgttt catcgagggg cctgacaggg
     2041 atcccgaact gctgccctgg ttcgaggcac cgcacggacg cgacatcgcc cggctggtca
     2101 agagcttcgg cctgtatcac atccatgccg aggatgcggc ctcgttggcc gctggactgg
     2161 acgaactgta caaggatcat ggccgccccg ccgtattgca cgttaccacc gatgcacgga
     2221 catcgccgaa ggtgctgcgc ggatacttca aacagctccg ggaggcatga tccggaccgg
     2281 gcggccgatc gcccccaatg aacccgatat gaggaactgg accaccatca aggaatacgc
     2341 cgacatccgt ttcgacctgt tcgatgggat cgcgcggatc acgatcaacc ggcccgaggt
     2401 gtacaacgcc ttccgtccgg agacgaacat ggagatgatc gatgcgatgg acatcgcgcg
     2461 tgaggatacc cggatcgggg tagtggtgct gacgggtgcg ggcgacaagg ccttctgcag
     2521 cggcggcgac cagaacgcga agggacgcgg cggctacatc ggcagcgacg gggtcccccg
     2581 actgaacgta ctggacctgc acaagcgcat ccgcgaactg cccaaacccg tggtcgccat
     2641 ggtgaacggc tacgccatcg gtggcggcca tgtgctgcat gtggtgtgcg acctcaccat
     2701 cgccgccgac catgcgcgtt tcgggcagac cggtcccaag gtgggcagct tcgatgcggg
     2761 cttcggcagc aactacctgg cgcgccacgt gggccagaag aaggcgcgcg agatctggtt
     2821 cctgtgcttg caatactccg cgcaggaggc gctggacatg ggcatggtga acaaggtggt
     2881 gccgctggcc gagctggagg acaccacggt ggaatggtgc aggatcatga tgcagcgcag
     2941 tccgctcgcc ctgcgcatga tcaagcgcgg cctcaacgcc gagctcgacg gccagcgcgg
     3001 cctgatggag ttcgccggtg atgccacgct gatgtactac ctgatggatg aggcgcagga
     3061 gggccgcaac gccttcctgg agaagcgccc gcctgacttc cagaagttcc cgaagttccc
     3121 gtgaggcgga aggaccgtaa cgacgcgaag gcacaacggg catgagctgc cggcccactt
     3181 accggacctg cagcgtgcac cacttggggc tacagaccgc attgcccatc tttgatgcat
     3241 gatgggcata c
//
LOCUS       DAOQOC010000203         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14833_length_3248_cov_3.347635, whole genome shotgun sequence.
ACCESSION   DAOQOC010000203 DAOQOC010000000
VERSION     DAOQOC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14833_length_3248_cov_3.347635"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..1614
                     /locus_tag="PL106_04395"
                     /pseudo
     CDS             460..1614
                     /locus_tag="PL106_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(2023..3144)
                     /locus_tag="PL106_04400"
     CDS             complement(2023..3144)
                     /locus_tag="PL106_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNA32341.1"
                     /translation="MAAQEEDNVLKGFSLGRILLPVVIGIGTTLFLVWKASNKAGDQP
                     IVWTSDTTGWMLLALLSLFVRDWMYMLRIRHLTDNGLSWWRAFVVIMLWEFSSALAPG
                     IIGGGFFFAIWILSREGIDAGKSISVITFTSFLDGIFVVVMAPLVYFTVGREELFGSM
                     DTSTDLTGLGAFVIFWLVFTIIVVYKLFVAYALFINPHFVKRALVGIFSINGLRRWRR
                     SMVTTGDQLIVAAAELRKRGWDYWWPGIATTFLSWTARFSIVNCIIHAFHPGMGFSND
                     AEMFGKQVIMGIIVLLMPTPGGSGIAEYMFRNFLGMYITVGPDKLAFVWRLLSYYPYI
                     AIGAFLLPRWVKRNVVDPLAHRKGAGIPDPDRPTTLPSE"
BASE COUNT          686 a         1047 c          932 g          583 t
ORIGIN      
        1 cgatgatggg agaatcggga tcctgaggag gtcttacggc gttcagttcg atccgtttca
       61 tggtctcaca atttgcatgc cgtgcgaagg tccgaggcgt tttgctcagc tcgaacattc
      121 tgaatctcgc tctggatacg ggttcaagtt ttccacctgt catataattt atattatgtt
      181 aagtcatgtg agcggattgg tccacgatcc tgttgcactt gatccgagct atccgcatgc
      241 gctataggcc tcatgaaatc ctgctgcttc acttccccct acatttgtcc cgccctaagc
      301 gctcagcttt cgctcaccgg tcagcatgaa ccggttcctc gactgtcacg gcgccatccc
      361 cagcggatca ttgtgcgccg gaccgatcgc agggctttta ccaacaccta ccgtgcgggt
      421 ggcaccaatg ggctcttcgc gcacttgaac gcataccacg tgcatcaacc ctcctttgat
      481 cagctcgatc tgatcgaccc catccgcaag gcgctccaag cggaaggcta ttccacgcca
      541 acgcccatcc aggcacaatc cattccttac ctgctcgaag gccgcgacct gctcggctgt
      601 gcgcagaccg gtacgggcaa aacggccgcc ttcgccattc cgctcatcca gcggctctcc
      661 accagcatcc aagcggtcga tgcacctgct tcgcgtgggg ccaggcgccc gatcaaggcc
      721 ctgatcctta caccaacacg cgagctggcc atccagatcg aggaaagctt ccgcgcctat
      781 ggccgtttca cccctctgaa atacaccgtg atcttcggcg gcgtggggca aaaaccgcaa
      841 acggatgcac tgcgggctgg cgttgatgtc ctgatcgcca cgccgggtcg actgctcgac
      901 ctgatgagcc aagggtacgt acacttgaag gatctggagt acttcgttct ggacgaggcg
      961 gaccgcatgc tggacatggg cttcatccat gatgtgcgca aggtgatctc cgcccttcca
     1021 tccaagcgcc aaacgctgtt cttcagcgcc acaatgccgc ctgagatcca gcgactttcg
     1081 gcctctatcc tcgccaatcc cgtgaaggtt gaggtcaccc cggcctccag caccgctgaa
     1141 acggtgcaac agcaggtgta tttcacggac aaacccaaca aggccgccct cctcacccac
     1201 ctgatgcgcg aaatggacat tcgtcaggtg ctcgtgttca cccgtacaaa gcacggtgcc
     1261 gacaaactcg ccaggttgct gatgaaggct ggcatcacag ccgacgccat tcacgggaac
     1321 aaggcgcaga actaccgcca gcgcgcgctg aacaacttca aggacaagaa gatccgggcc
     1381 ttggtggcca cggacatcgc ggcgcgcggt atcgacatcg atatgctggg cttcgtggtg
     1441 aattacgaga tcccgaacgt gcccgagacc tacgtacacc gcatcggccg caccggtcgt
     1501 gcgggcgcca gcggtttggc catcagccta tgcgaccagg aggaagtgga gtacctgcgg
     1561 gatatccaac gcctgatcaa gcgcgacgtg ccggtggtgg agcaacatcc cttccccatg
     1621 gccggcggaa ccccggtggt ggacccgggt cgaggaaaac ggccacagca acagcatgca
     1681 cagcggccac cgcagccgcc acgaccggaa cgtcccgctc cacgacgacc cgaccggaac
     1741 gaagctccac gtgggcagaa gtcgccggac ggtcgacaca cggacaaagg ccaccaccct
     1801 cgcccggagc gtgagcgcag gcccgaccag gctgcgggca ccgcctccaa gcaggattat
     1861 gcgtccctca cccgcgatct gatcgccgag gtggatgcgt tcctgggcgg gaaaaaacca
     1921 atcggtgagc agcacgcgaa cccgaagcag tccggtgaac atcgccgtac cgaagggcaa
     1981 ggtcgtcgtc gttggcgagg accgcggcgg taaaccccgc tgttattccg agggaagcgt
     2041 ggtcggccgg tccggatcgg ggatcccggc ccctttcctg tgtgccaacg ggtccaccac
     2101 attgcgtttc acccagcgcg ggagcaggaa ggccccgatg gcgatgtacg gatagtagct
     2161 caacaagcgc cagacgaacg cgagcttgtc gggaccgacc gtgatgtaca tgcccaggaa
     2221 attacggaac atgtattcgg cgataccgct gccgcccggc gtgggcatca acaggacgat
     2281 gatgcccatg atcacctgct tcccgaacat ctccgcgtcg ttactgaaac ccattcccgg
     2341 gtggaacgca tggatgatgc agttcacgat ggagaacctg gccgtccagc tgaggaaggt
     2401 ggtggcgata cctggccacc agtagtccca gccgcgcttg cgcaattcgg cggcggcaac
     2461 gatcagttga tcgcccgtgg tgaccatgct cctgcgccac cgccgcagcc cattgatgga
     2521 aaaaatgccc acgagggcgc gcttcacgaa gtgcgggttg atgaaaagcg cataagccac
     2581 gaagagcttg tacacgacga tgatggtgaa gaccagccag aagatcacga acgcgcccag
     2641 cccggtcagg tcggtactgg tgtccatgct gccgaacagt tcctcgcgcc ctaccgtgaa
     2701 gtagaccaga ggcgccatca ccaccacgaa gatcccgtcc aggaacgagg tgaaggtgat
     2761 cacggaaatg cttttgcccg cgtcgatgcc ctccctgctc aggatccaga tggcgaaaaa
     2821 gaagccgccg ccgatgatgc ccggcgccag cgcgctggag aattcccaca gcatgatcac
     2881 cacgaatgca cgccaccagc tcaggccgtt gtccgtcagg tgccggatcc gcagcatgta
     2941 catccagtcg cgtacgaaaa ggctgagcaa ggccaggagc atccatcccg tggtgtccga
     3001 ggtccagacg atcggctggt ccccggcctt gttgctggcc ttccacacca ggaacagcgt
     3061 ggtgccgatg ccgatcacca ccggaagcag gatgcgaccg aggctgaagc ccttcagcac
     3121 attgtcctcc tcctgcgccg ccatgcggta aacataggtc ggagccggga gtggtgcgtg
     3181 gggccgttgg tcagggcgct gcccgatcag ttcgtaccct cccgccgctt caactcatcc
     3241 cggagttt
//
LOCUS       DAOQOC010000204         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14854_length_3246_cov_2.924475, whole genome shotgun sequence.
ACCESSION   DAOQOC010000204 DAOQOC010000000
VERSION     DAOQOC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14854_length_3246_cov_2.924475"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PL106_04405"
     CDS             complement(<1..349)
                     /locus_tag="PL106_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32342.1"
                     /translation="MRSCATTLILCGCACASLGQQNDVPLNRDIYYDIDRNGACRTST
                     MHTGLRPMIESRADLDNVMGFRPDKGRHYYLFTKKLFAEHLINARHGGFKLSIDPVFR
                     FEMGDPTATQSAKA"
     gene            432..818
                     /locus_tag="PL106_04410"
     CDS             432..818
                     /locus_tag="PL106_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HNA32343.1"
                     /translation="MRFPQERNDHLVNLLTIARFDPSSENRAAVQEELLRGRALLIVQ
                     ARPVPGDPRSLEFLHAPVLDGRPTLFAFTSEVALRSWSTQDMELVIVPAGAFFQGCLK
                     NGIGTVVVDASTPNELRIALGRSAAA"
     gene            complement(819..1757)
                     /locus_tag="PL106_04415"
     CDS             complement(819..1757)
                     /locus_tag="PL106_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNA32344.1"
                     /translation="MNKTDKIFVAGHRGMVGSAIVRRLQKDGFTNIVTRTSKELDLRE
                     QQPVRDFFASEKPDIVVLAAAKVGGIHANNVYRAQFLHENLMIQNNVIDSAYRNGVKK
                     LLFLGSSCIYPKMAPQPLKEESLLTGLLEQTNEPYAIAKIAGIKMAESYRRQYGVNYI
                     SAMPTNLYGPNDNYDLNNSHVLPALIRKFHTAKVTNAPSVEVWGTGSPMREFLHVDDL
                     ADACFFLLQNYDEELFVNIGTGEDLTIKALAEMIKEIVGYSGELKWNTEKPDGTPRKL
                     MDVSRLHKLGWKHRIGLREGITAVYEEFAKSELAKA"
     gene            complement(1867..2982)
                     /gene="gmd"
                     /locus_tag="PL106_04420"
     CDS             complement(1867..2982)
                     /gene="gmd"
                     /locus_tag="PL106_04420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA32345.1"
                     /translation="MAKKKASGKKRKVALITGVTGQDGAYLSEFLLNKGYEVHGVKRR
                     SSLFNTDRIDHLYHDMHEQGRPFHLHYGDLTDSVNCLRLVKEIQPDEIYNLAAMSHVA
                     VSFEMPEYTANADGIGTLRFLEAIRILGMEKKTRFYQASTSELYGGVLPRAQNEETPF
                     YPKSPYGVAKLYGFWITKNYRESYNIFACNGILFNHESPLRGETFVTRKITRAVAKIK
                     LGLQQTLYLGNMDAKRDWGHAKDYVEGMWLMLQHDKPDDYVLATGKTYTVRHFIELAF
                     AEVGIRIEWKGKGEKEKGIDKKTGKTLVAIDKRYYRPAEVDHLEGDPRKAQRVLGWKH
                     KYDLKALVKEMVSSDVELFKRDVYLKKGGHRILHEQE"
     gene            complement(3022..>3246)
                     /locus_tag="PL106_04425"
     CDS             complement(3022..>3246)
                     /locus_tag="PL106_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNA32346.1"
                     /translation="GDVALTSMGSALKICLVAEGAADAYPRYAPTMEWDTAAGQAIAN
                     ETGKALIDVTTDAPMRYNKHTLVNNWFIVQ"
BASE COUNT          520 a          991 c          989 g          746 t
ORIGIN      
        1 tcgccttcgc cgattgcgtc gccgtggggt cgcccatctc gaagcggaac accggatcga
       61 tgctcaactt gaaccccccg tgtctggcgt tgatcaggtg ctcggcgaac agcttcttgg
      121 tgaacaggta gtagtgtcgc cccttgtccg gccggaagcc catcacgttg tccagatcgg
      181 cccgactctc gatcatgggt cgcaggcccg tgtgcatggt gctcgtgcgg caggccccgt
      241 tccggtcgat gtcgtaatag atgtcccggt tcaaaggcac gtcattctgc tggccgaggc
      301 tcgcacaggc gcagccgcac aggatcaggg tggtcgcgca ggaacgcatg atgccgggcg
      361 ccgaaggtag aaggacggcc caacccgcga cctgctccat gcgtacaccg tacgcccttc
      421 gagggtccac catgcgcttt ccgcaagaac gcaatgatca cctggtgaac ctgctcacca
      481 tcgcccggtt cgacccatcc tccgagaacc gcgcagccgt gcaggaggaa ttgttgcgtg
      541 gccgtgcttt gctcatcgtt caggctcgtc ccgtaccggg tgatccgcgc agtctggagt
      601 tcctgcacgc accggtgctc gatggccgcc ccacgctgtt cgccttcacc agcgaggtgg
      661 ccttgcgcag ctggtccacg caggacatgg aactggtgat cgtgccggcc ggggcatttt
      721 tccaaggctg tctcaagaac gggatcggta ccgtggtggt ggatgcgagc acccccaacg
      781 agttgcgcat cgcactgggc cgttcggccg ctgcctgatc aggctttcgc cagctcgctc
      841 ttggcgaact cctcataaac cgccgtgatc ccctcgcgca agccgatgcg gtgcttccac
      901 cccaacttgt gcaagcgcga aacatccatc agtttacgcg gcgtaccatc gggcttctcg
      961 gtgttccact tcagttcacc gctgtagccc acgatctcct tgatcatctc ggcgagcgcc
     1021 ttgatcgtga ggtcctcgcc cgtaccgatg ttcacgaaca gttcctcgtc gtagttctgc
     1081 agcaggaaga agcaggcgtc ggccaggtcg tccacgtgca ggaactcgcg catgggcgag
     1141 cccgtgcccc agacctccac actcggggcg ttcgtgactt tcgccgtgtg gaacttgcgg
     1201 atgagcgcgg gcagcacgtg gctgttgttc aggtcgtagt tgtcgttggg cccgtacagg
     1261 ttggtgggca tcgcactgat gtagttcacc ccgtactgac ggcggtagct ctcggccatt
     1321 ttgataccgg cgatcttggc gatcgcgtat ggctcgttcg tctgctcgag cagcccggtg
     1381 agcaggcttt cctccttcaa cggttgcggg gccatcttcg ggtatataca cgaggagccc
     1441 aggaacagca gcttcttcac gccgttgcgg taggcgctgt cgatcacgtt gttctggatc
     1501 atcaggttct cgtgcaggaa ctgcgcgcgg tacacgttgt tggcatggat gccgccgact
     1561 ttcgccgctg cgagcaccac gatgtccggc ttctcactgg cgaagaagtc gcgtacgggc
     1621 tgctgctcgc gcaggtcgag ttccttgctg gtgcgggtga cgatgttggt gaaaccgtcc
     1681 ttctgcagac ggcgaacgat ggccgagccc accatgccgc ggtggccggc gacgaagatc
     1741 ttgtctgttt tgttcatctg tcgaggcgac gttcacgtcg tccgggattt tgaaggtcgt
     1801 ccttgaggga tccgcacgga tcggggcgac atgaatgtcg tccctgcgga atgatcgcga
     1861 atgcgatcat tcctgttcgt gcaggatcct atgccctccc ttcttcaggt acacgtcgcg
     1921 cttgaaaagc tccacatcgc tgctcaccat ttctttcacc agggccttga ggtcgtactt
     1981 gtgcttccag ccgagcaccc gctgggcctt gcgggggtcg ccctccaggt ggtccacttc
     2041 cgccgggcgg tagtagcgct tgtcgatggc caccagggtc tttccggttt ttttatcgat
     2101 gcccttctcc ttctcgccct tccccttcca ctcgatcctg atgcccacct cggcgaacgc
     2161 gagctcgatg aagtggcgca cggtgtacgt cttgcctgtg gcgagcacgt agtcgtccgg
     2221 tttgtcgtgc tgcaacatca gccacatgcc ctccacatag tccttggcat ggccccagtc
     2281 gcgcttggcg tccatattgc cgaggtagag tgtttgctgc aggccgagct tgatcttggc
     2341 cacggcgcgc gtgatcttgc gggtgacgaa ggtctcaccg cgcagcgggc tctcgtggtt
     2401 gaacaggatg ccgttgcagg caaaaatgtt gtagctctcg cggtagttct tggtgatcca
     2461 gaagccgtag agtttggcca cgccgtacgg gctctttggg tagaacggcg tttcctcatt
     2521 ctgcgcacgg ggcagtacgc cgccatacag ttctgaagtg gaggcctggt agaagcgggt
     2581 cttcttctcc atacccagga tccgtatggc ttccaggaag cggagcgtac cgataccgtc
     2641 ggcgttggcc gtatactcgg gcatctcgaa gctcaccgcc acatggctca tggcggccag
     2701 gttgtagatc tcgtccggtt ggatctcctt cacgagccgg aggcagttca cgctgtcggt
     2761 gaggtcgccg taatgcaggt ggaacgggcg cccctgctca tgcatgtcat gatagaggtg
     2821 gtcgatgcgg tccgtgttga acagggaact gcggcgtttc acaccgtgta cctcataccc
     2881 cttgttcaac aggaattcgc tgaggtaagc accgtcctga ccggtcacac cggtgatcag
     2941 ggccacttta cgctttttgc cggatgcctt cttcttcgcc atttcgtctg ggcgacgctt
     3001 ggatcgccgt gattttggtt gctattgaac gatgaaccag ttgttcacca gtgtgtgctt
     3061 gttgtatcgc atcggcgcat cggtggtcac atcgatgagc gccttgcccg tttcattggc
     3121 gatggcctgc ccggccgcgg tgtcccactc catggtgggc gcgtagcgcg ggtaggcgtc
     3181 agccgcgcct tcggccacca ggcagatctt cagggcactg cccatgctgg tgagggccac
     3241 atcgcc
//
LOCUS       DAOQOC010000205         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14880_length_3243_cov_3.797679, whole genome shotgun sequence.
ACCESSION   DAOQOC010000205 DAOQOC010000000
VERSION     DAOQOC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14880_length_3243_cov_3.797679"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..573)
                     /gene="rplK"
                     /locus_tag="PL106_04430"
     CDS             complement(130..573)
                     /gene="rplK"
                     /locus_tag="PL106_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNA32347.1"
                     /translation="MAKEISGLVKLQVKGGAANPAPPIGPALGAKGVNIMEFCKQFNA
                     RTQDKAGKVLPVVITVYSDKSFDFIIKTPPAAVQLMDAAKVKGGSGVPQTKKVGSVTW
                     DQVKAIATDKMPDLNCFTIESAMSMVAGTARSMGITVQGESPLKK"
     gene            complement(712..1272)
                     /gene="nusG"
                     /locus_tag="PL106_04435"
     CDS             complement(712..1272)
                     /gene="nusG"
                     /locus_tag="PL106_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNA32348.1"
                     /translation="MATAATSTRKWYVLRAISGKEKKVKEVIDAEVRRSNMGTYISQV
                     LIPVEKRFLIKDGKKVSKEHNMLPGYVLIEADLTGEIAHALKQLPNVIGFLGAEKGGE
                     PVPMRVSEVNRILGKVDEMAEGGEVNHNPFHIGESVKVIDGPFNTFTGIIEEINEEKK
                     KLKVMVKIFGRKTPLELSFMQVEKEV"
     gene            complement(1275..1502)
                     /gene="secE"
                     /locus_tag="PL106_04440"
     CDS             complement(1275..1502)
                     /gene="secE"
                     /locus_tag="PL106_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNA32349.1"
                     /translation="MASLKTYFAESYNELMHKVSWPTWKELQSSAIIVLFSALLIAFI
                     VFLMDFLFGVQNLENSNPWKGLVGFVYQFLG"
     gene            complement(1518..1593)
                     /locus_tag="PL106_04445"
     tRNA            complement(1518..1593)
                     /locus_tag="PL106_04445"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1558..1560),aa:Trp,seq:cca)
     gene            complement(1683..2870)
                     /gene="tuf"
                     /locus_tag="PL106_04450"
     CDS             complement(1683..2870)
                     /gene="tuf"
                     /locus_tag="PL106_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNA32350.1"
                     /translation="MAKDTFKRDKPHVNIGTIGHVDHGKTTLTAAITNVLASKGLSEV
                     RSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPKIVVFMNKVDSVDDAELLDLVEMEIRELLTF
                     YQYDGEKTPVIRGSALGALNGEPKWVDTVMALMDAVDTWIPVPPRETEKPFLMSVEDV
                     FSITGRGTVATGRIETGVVKVGDNVEIIGMQEQKLTSTCTGVEMFRKLLERGEAGDNC
                     GLLLRGIEKDQIRRGMVIAAPGSITPHTEFKGEIYVLKKEEGGRHTPFHNKYRPQFYF
                     RTTDVTGEITMEAGREMIMPGDNVSITVKLIVPIAMDKGLRFAIREGGRTVGAGQVTE
                     IIK"
     gene            complement(2969..3043)
                     /locus_tag="PL106_04455"
     tRNA            complement(2969..3043)
                     /locus_tag="PL106_04455"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3009..3011),aa:Thr,seq:ggt)
     gene            complement(3098..3170)
                     /locus_tag="PL106_04460"
     tRNA            complement(3098..3170)
                     /locus_tag="PL106_04460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3135..3137),aa:Gly,seq:tcc)
BASE COUNT          511 a          968 c          958 g          806 t
ORIGIN      
        1 gtcgaacttg gtcgtgctca cttgcttcac gatctgggcg gcttgggcca ggtcgtacaa
       61 cttggtaccg tcgaacttgc tctcggcggc cttgcgcttc ttggtcaggt ttgccatggc
      121 tgttcgcggt tatttcttga gggggctttc accttggacc gtgatgccca tgctgcgtgc
      181 ggtgccggcc accatgctca tggcgctctc gatggtgaaa caattcaggt cgggcatctt
      241 gtcggtggcg atggccttta cctgatccca ggtaacgctg cccaccttct tggtctgggg
      301 cacaccgctg ccgcccttca ccttggccgc gtccatcagc tgcacggctg cgggcggggt
      361 cttgatgatg aagtcgaagc ttttgtcgct gtacacggtg atcaccacgg ggaggacctt
      421 gcccgccttg tcctgcgtac gcgcattgaa ctgcttgcag aactccatga tgttcacccc
      481 tttggcaccc agtgcgggtc cgatcggggg cgcggggttc gccgccccgc ctttcacctg
      541 gagtttcacc aggccgctta tctcctttgc cattcttctg gttttgtgtc cttgttagcg
      601 ccttcccgca ggtcggctcg gacggttgtt cttttctata tctcttgtcg ggtcgacgtg
      661 aacgtcgacc tggtgttgtt ctttctggtc gggtagacgt gaacgtcgac cttacacttc
      721 tttttctacc tgcatgaagc tcagttcgag cggggtcttc cgcccgaaga tcttcaccat
      781 cactttgagt tttttcttct cctcgttgat ctcctcgatg atgccggtga aggtgttgaa
      841 gggcccatcg atcaccttca cgctctcacc gatgtggaac gggttgtggt tcacctcccc
      901 tccctcggcc atttcatcca ccttgccgag gatccgattg acttcggaaa cgcgcatggg
      961 cacgggctcc ccgccttttt ccgcaccaag gaaaccgatc acgttgggca gttgcttgag
     1021 cgcgtgcgcg atctcgccgg tgaggtcagc ctcgatgagc acatagccgg gaagcatgtt
     1081 gtgctccttg ctcaccttct tcccgtcctt gatgaggaag cgtttttcca cgggaatgag
     1141 cacctggctg atgtacgtgc ccatgttgga gcggcgcacc tccgcatcga tcacctcctt
     1201 cacttttttc tccttgcctg agatggcacg caggacgtac catttccggg tggatgtcgc
     1261 ggcggtggcc atgttcatcc gaggaattgg taaacgaaac cgacaaggcc cttccacgga
     1321 ttgctgttct ccaggttctg caccccgaac aggaaatcca tcaggaacac gatgaaggcg
     1381 atgagcaggg cggagaacag gacgatgatg gcactgctct ggagttcctt ccaggtgggc
     1441 cagctcacct tgtgcatgag ctcattgtag ctctcggcga aatatgtctt gaggcttgcc
     1501 acgtgggatc ttgttgttgg cacgggtggc aggaatcgaa cccgcagcct aaggttttgg
     1561 agaccttcgc tctaccaatt gagctacacc cgtgtgtgtc ttccgtgctg gtccattttc
     1621 ggcggcgaag gccgtcccgc tcatcgcggg acggccctgc ctccattgtt ctggtatgcg
     1681 gcttacttga tgatctcggt cacctgtccg gcacccacgg tacgaccacc ctcgcggatg
     1741 gcgaagcgaa ggcccttgtc catggcgatc ggaacgatca gtttcaccgt gatgctcacg
     1801 ttgtcgcccg gcatgatcat ctcacgaccg gcctccatgg tgatctcgcc cgttacgtcc
     1861 gtggtgcgga agtagaactg ggggcggtac ttgttgtgga agggggtgtg acgaccgccc
     1921 tcttctttct tcagcacgta gatctcgccc ttgaactcgg tgtgcggggt gatgctgccc
     1981 ggggcggcga tcaccatgcc acggcggatc tggtcctttt caataccgcg gaggaggagg
     2041 ccacagttgt cgccggcctc accgcgctcc aggagcttgc ggaacatctc cacaccggtg
     2101 caggtgctgg tcagtttctg ctcctgcatg ccgatgatct ccacgttgtc gcccaccttc
     2161 accacaccgg tctcgatccg gccagtggcc acggtaccgc ggccggtgat ggagaacaca
     2221 tcttccacgc tcatcaggaa gggcttctcc gtttcgcgcg ggggcacggg gatccaggtg
     2281 tcaacggcat ccatcagggc catcacggta tccacccact tgggctcacc gttcagggcg
     2341 cccagagcgc tgccacggat caccggggtc ttctcgccat cgtactggta gaaggtgagg
     2401 agttcgcgga tctccatctc aacgaggtcg aggagttcgg cgtcatccac gctgtccacc
     2461 ttgttcatga acaccacgat cttgggcacc cccacctgac ggccgagcag gatgtgctcg
     2521 cgggtctggg gcatggggcc gtcggtggcg gccaccacca ggatggcacc gtccatctgg
     2581 gcggcaccgg tcaccatgtt cttcacatag tcggcgtggc ccgggcaatc cacgtgggcg
     2641 tagtgacggt tggccgtctg gtactccacg tgcgatgtgt tgatggtgat accgcgctcc
     2701 ttctcctcgg gcgcgttatc gatcgagtcg aagctgcgca cttcgctcag gcccttggag
     2761 gcgagcacat tggtgatcgc cgcggtgagc gtggtcttgc catggtcaac gtggccgatg
     2821 gtgccgatgt tgacgtgcgg cttgtccctt ttgaaggtat cctttgccat ggtcgatgat
     2881 tgtcgttggg ttgttctgtt ctggtgttcc tgggggatct tctcccactg tacctgccgc
     2941 cgtagatctt tctggtgcct tttcctcctg gagcctttga cgggaattga acccgtgacc
     3001 tcttccttac caaggaagtg ctctacctct gagctacaaa ggcctgatgc gctgttcttg
     3061 gtattgttcc cctggggcgc cggccccgtg ggcgtagagc gggagacgag attcgaaccc
     3121 gcgacattca gcttggaagg ctgatgctct accaactgag ctactcccgc gtaatggtca
     3181 ttcgttccgg gtatttccga tcggatgggg ccgcccgcgg gctgaagacc cgttggaagc
     3241 tca
//
LOCUS       DAOQOC010000206         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14958_length_3234_cov_3.496383, whole genome shotgun sequence.
ACCESSION   DAOQOC010000206 DAOQOC010000000
VERSION     DAOQOC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14958_length_3234_cov_3.496383"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1154)
                     /locus_tag="PL106_04465"
     CDS             complement(267..1154)
                     /locus_tag="PL106_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32351.1"
                     /translation="MRNHHLTLLFLLTMTANATDRIVAPGATLPRYASISAALSAAAD
                     NDRILVVPSIYTENLTIDKSVEILSMVPGRRYTATGNLLIAPPVQTVKRIVVHDLELL
                     GNISTGGQVNHPNIIVLTGCRTGRIDFGLNFSWRIALANDSILGRLFLPGGTLVGSYV
                     RGDTLGGGGFKVLTSTGSNRPLVLIGNILGDPYEADKPIGSCAIDLFSADTIRFENNH
                     VRLYREHYVSEPICLNSAYNTSASAVRLLNNTFIRSATAGSVGPIIADQTIGYVESRN
                     NFFLGPITAPIFSHPYTDE"
     gene            complement(1167..2360)
                     /locus_tag="PL106_04470"
     CDS             complement(1167..2360)
                     /locus_tag="PL106_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32352.1"
                     /translation="MKPLPAPLLSALVLFILTRISAQATDRVVVPGGMPPVYPTIAAA
                     LNAASDNDRILVTPATYTGTITIAKSVELTSTVSGQRYTVTGDLVIAPGPGHKRIAVH
                     GLVLSGMIGGATDPNPHSFTLTGSTTGRIDVQGLYRTRCTLLRDTVNGSVRMRGGDLI
                     GCRITGGDDHPGADHFGVLLMWGEEGNTATCNVIGNHMGSIAELADANGSLPVSITAA
                     SPVYFANNYVELFGAAYLNRPIACYTPDFDQIERFRFVNNTVRWVNVGSELQDLIWAD
                     GQTVYAPIDARNNLLIGPLRIAVSGNTSSQGFNLQTMDSGQINISTGRPIPASIAWDS
                     GDPDASFTDLDLSRNDVGCYGGSMSRDQFDDPVMNLAQATWTDAQHRVITLTPTAVVT
                     QAVDR"
     gene            complement(2370..>3234)
                     /locus_tag="PL106_04475"
     CDS             complement(2370..>3234)
                     /locus_tag="PL106_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32353.1"
                     /translation="LSAIGDLTETAPMGVGAMVTMVGCRLSRVEMLMGQGRMALYRDS
                     IFGEVVILRGDVIGCYVSGTPDSGGILITGGSMSTETNRVIGNDIGAAMTNTSFWPIM
                     IQPCAPFICENNLLRINMTNSSSFPLFVPNAPTPSTMPCTIRNNTFRYPDHPNAYFIK
                     IEQSAPSFTLDIRNNLFVGPVPIDPIDVVPGSSTTEAYNVLSFDLGSIDPVSGVPTSG
                     SPAINAGDPDAQYTDLDLTRNDAGCHGGSSSRDNFDDPLPTTAVICFVNAPRRTQTAL
                     SIPISAEGTDR"
BASE COUNT          630 a          914 c         1052 g          638 t
ORIGIN      
        1 tgatccgtac aagaagcgtc gatcccgacc ggcacactac tgaagacgcg gcgaacaaca
       61 tgactccaca ggatttccgc tgtgcccgtc ggaggatcat cgaacaggtc ccgactgaac
      121 gagccgccat aacacccggc gtcgttacgg ctcagatcca agtcgttgta gcggccgtcc
      181 ggatccccga cgtccgtgca gatggatcct gcgatcggac ggccagtggc cggatccacc
      241 tgttcttggt ccgtactgaa aaggtttcat tcatccgtgt agggatggga gaatatgggc
      301 gccgtgatcg gaccgagaaa gaagttgttc cgggactcca cgtacccgat ggtctggtcg
      361 gcaatgatcg gcccgacgga accggccgta gcggatctga tgaatgtgtt gttcaataga
      421 cgcacggcgg aggcggaggt attgtaggcg ctgttcaggc agatgggctc ggacacgtag
      481 tgctcccgat agagccgaac atggttgttc tcgaaccgga tcgtgtctgc actgaacagg
      541 tcgatcgcac aggatccgat cggtttgtcc gcttcgtaag gatcgcccag gatgttaccg
      601 atgagcacga gcggtcggtt gcttccagtg gaggtgagta ccttgaaacc gcccccaccg
      661 agggtgtctc cgcgcacgta ggaaccgacc agcgtgccac ccggtaggaa caaccgcccg
      721 aggatgctgt cgttcgccag cgcgatccgc cagctgaaat tcagcccgaa gtcgatgcga
      781 cccgttcgac agccggtgag cacgatgatg ttcggatggt tcacttggcc acccgtgctg
      841 atgttaccga gcagttccaa gtcgtgtacc acgatacgct taaccgtttg cacgggtggc
      901 gcgatcaaca agttgcccgt ggcggtatac cgtcgcccag gtaccatgct caggatctcc
      961 acgctcttgt cgatggtcag gttctccgtg tagatcgacg gcaccacgag gatccggtcg
     1021 ttgtccgccg cagcggaaag ggccgccgat atcgaggcgt agcgtggcag ggttgctccg
     1081 ggcgcgacga tccggtccgt cgcattcgcg gtcatggtca gcaggaagag aagggtgagg
     1141 tggtggttcc gcatggtgat gtgcgatcag cggtcaacgg cctgggtgac gacggcggtc
     1201 ggggtgaggg tgatcacgcg gtgttgagca tccgtccaag ttgcctgggc caagttcatc
     1261 acgggatcgt cgaactggtc gcggctcatg gaccctccat agcaaccgac gtcgttccgg
     1321 ctcaggtcca ggtcggtgaa tgaagcgtcc gggtctccgc tatcccatgc gatggacgcg
     1381 ggtatcggcc gtccggtcga gatgttgatc tgcccactgt ccatggtctg caggttgaac
     1441 ccctgcgaac tggtgttccc gctcacggcg atgcgaagtg gtccgatcaa caggttgttc
     1501 cgcgcatcga tcggtgcgta cacggtctgt ccatcggccc agatcaggtc ctggagctcg
     1561 ctgccgacat tgacccatcg gaccgtattg ttcacgaatc ggaagcgctc gatctgatcg
     1621 aagtctggtg tgtaacaggc gatgggccgg ttcagatagg ccgcgccgaa gagttccacg
     1681 tagttgttcg cgaagtacac gggactggcc gccgtgatgg acaccggtaa gcttccgttc
     1741 gcatcggcca gctccgcgat cgatcccata tggttgccga tcacgttgca ggtggcggtg
     1801 ttgccttctt caccccacat gagcagcacg ccgaagtggt cagcacccgg gtggtcatcg
     1861 ccgccggtga tacggcaacc gatcaggtcg ccgccccgca tgcgcaccga gccgttcact
     1921 gtatcacgta gcagcgtgca gcgcgtccgg tacaggccct ggacatcgat ccgaccggtg
     1981 gtgctaccgg tcaaggtgaa ggagtgcgga ttaggatcgg tggctccacc gatcataccg
     2041 ctgagcacca gaccatgcac ggcgatcctc ttgtgaccag gcccgggcgc gatcaccagg
     2101 tcgccggtca cggtataccg ctgtccgctc acagtgctcg tcaactcgac gcttttcgca
     2161 atggtgatgg tgccggtgta cgtggcgggg gtcaccagga tgcggtcatt gtccgatgcg
     2221 gcgttcaatg cggcggcaat ggtcgggtac accgggggca taccaccggg aacgaccacg
     2281 cggtcggtgg cctgtgccga gatacgggtc aagatgaaca ggacgagcgc agagagcaaa
     2341 ggagcgggca acggtttcat ggggaggctt cagcgatcgg taccttcggc ggagatcgga
     2401 atggagagcg cggtctgggt gcggcgcggt gcgttcacga agcagatcac cgccgtggtc
     2461 ggcaatggat catcgaaatt gtcacggctc gagcttccgc catggcagcc ggcgtcgttg
     2521 cgcgtgaggt cgaggtccgt gtactgtgca tccgggtcgc ccgcgttgat ggcaggcgat
     2581 ccggaagtgg gcacgcccga aacgggatcg atactgccca ggtcgaagga cagcacattg
     2641 tatgcttcgg tagtggatga accaggtacc acatcaatgg ggtcgatagg gacggggccc
     2701 acgaaaaggt tgttgcgaat gtccaatgtg aacgaagggg cggattgttc gatcttgatg
     2761 aagtaagcgt tcggatgatc cggatatcgg aaggtgttgt tcctgatggt gcacggcatg
     2821 gtgctgggtg tgggcgcgtt cggtacgaat agcggaaaag aagaactgtt ggtcatgttg
     2881 atccgcagca gattgttctc acagatgaac ggcgcgcacg gctggatcat gatcggccag
     2941 aaggaggtat tggtcatcgc agcgccgatg tcattcccga tgactcgatt ggtttcggtg
     3001 gacattgagc cgcccgtgat caggatcccg ccggagtccg gagtaccgct cacgtagcaa
     3061 ccgatcacgt cgccccgcag gatcaccacc tcgccgaaga tgctgtcgcg gtacaacgcc
     3121 atgcggccct gccccatcag catctccacc cgggagagtc gacatccgac catcgtgacc
     3181 attgcgccaa cccccatcgg cgcggtctcc gtgagatctc caatggccga cagt
//
LOCUS       DAOQOC010000207         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_14991_length_3230_cov_3.316850, whole genome shotgun sequence.
ACCESSION   DAOQOC010000207 DAOQOC010000000
VERSION     DAOQOC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14991_length_3230_cov_3.316850"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1611)
                     /locus_tag="PL106_04480"
     CDS             complement(205..1611)
                     /locus_tag="PL106_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNA32354.1"
                     /translation="MEPLLITIIVLALVFDYINGFHDAANAIATIVSTKVLTPFQAVL
                     WAAVFNFVAFFIFTDHAVANTISKTVHPEFITLPVILAGLLAAITWNLLTWWYGIPSS
                     SSHTLIGGFAGAAIAHAFEIMDGFALGDVVERDRILKIVAFILVAPLLGMAVSIGITL
                     VTLVTSTWYRITLIILGAIGIWIFFGDLQVNKMEENLSKYWKVDELAHAAEMDPAYMP
                     AYNAAKAKLEQAAPLLAMYGKMNSERLAAHIQKNVDPEVDVKKLGKALARADNFWLRA
                     GLMGMALAFILTYIYVEQVKTPNAFSMARMFKRLQLLSSAAFSIGHGGNDAQKVMGII
                     SAALISGGVIHDFKEMPSWVPLACYAAIGLGTLSGGWKIVKTMGTRITKVTPLEGVCA
                     ESAGALTLYMTEQMGIPVSTTHTITGCIIGVGTTKRASAVRWGVTVRLLWAWIITIPV
                     SALMAMFAYWVLGLFGLH"
     gene            complement(1616..2263)
                     /locus_tag="PL106_04485"
     CDS             complement(1616..2263)
                     /locus_tag="PL106_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNA32355.1"
                     /translation="MPFQAFFRLFKPKDRIFFYHFEASAANVLRMSEDLILMMASKRG
                     SQRDAILERLEMQERANDDLTHTIFTDLARNFITPIDREDIHQLATSLDDVADFILAA
                     AKNLELFGIEQPDATCQELARLVNEGCRIVQLAVQSLRDLHKSNTNIRFVVDINSMEN
                     EADGVYDKAIAHLFATETNAIDLIRWRDIYSTLELATDKCEDVANVIESIMLKYA"
     gene            2419..2910
                     /locus_tag="PL106_04490"
                     /pseudo
     CDS             2419..2910
                     /locus_tag="PL106_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
     gene            2944..>3230
                     /locus_tag="PL106_04495"
     CDS             2944..>3230
                     /locus_tag="PL106_04495"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HNA32356.1"
                     /translation="MWAAAPREKLERTGLTGWELDALPEVVTVDVGGRKLQAFPALVD
                     RETAVDVVLLESAAAAREATRAGLRRLYLLGMATTLTKLDGQLPAAIASGPL"
BASE COUNT          598 a          976 c         1061 g          595 t
ORIGIN      
        1 ggtgaagata ccgggccgca gtgcgcctac cggtctggta aaggtgagcg ggagcaggtg
       61 cttccagcgt ccggcatcgg aaaggatgat cgccatgcat gtgccggctt gcggctccgg
      121 ccaaatgtaa tcgggcgcat gggccggagt tgcgccttga tcaccgggat cggaggtcac
      181 gggtgcaggg atcccgaaca gccgctaatg caacccgaac agaccgagca cccagtaggc
      241 gaacatggcc atcagggcac tcaccggaat ggtgatgatc caagcccaga gcaggcgcac
      301 cgtaacaccc caacgcaccg cgctggcccg tttggtggtg cccaccccga tgatgcagcc
      361 cgtgatggtg tgcgtggtgc tcaccgggat gcccatctgc tcggtcatgt atagcgtcaa
      421 ggcaccggca ctctcggcac acaccccttc caatggcgtc accttggtga tgcgcgtgcc
      481 catggtcttc acgatcttcc agccaccgct gagggtaccc agaccgatgg cggcatagca
      541 ggccagcggc acccaactcg gcatttcctt gaaatcatgg atcacaccac cggagatcag
      601 cgcggcactg atgatgccca tgaccttctg cgcgtcgttg ccgccatggc cgatgctgaa
      661 agccgcagag gagagcaatt gcaggcgctt gaacatccgg gccatggaga acgcattcgg
      721 tgtcttcacc tgttccacgt agatgtaggt gagtatgaag gcgagcgcca tgcccatcag
      781 gcctgctcgg agccagaaat tgtcggcccg ggccagcgcc ttgcccagtt tcttcacgtc
      841 cacctcggga tccacgttct tctggatgtg cgcggccagg cgctccgagt tcattttgcc
      901 atacatggcc agcaagggcg cggcctgttc gagtttggcc ttcgcggcat tgtacgccgg
      961 catgtacgcc gggtccatct ccgccgcatg ggccagctcg tccactttcc agtacttgga
     1021 aagattctcc tccatcttgt tcacctgcag gtcgccgaag aagatccaga tgccgatggc
     1081 gccaagaatg atcagcgtga tccggtacca tgtggaggtg acgagcgtca ccagggtgat
     1141 gccgatgctg accgccattc cgagcagggg ggccacaagg atgaacgcca cgatcttcag
     1201 gatgcggtcc cgctccacca cgtcgcccag ggcgaaaccg tccatgatct cgaaagcgtg
     1261 ggcgatggcc gcaccggcga aaccgccgat cagggtgtgg ctgctgctgc tggggatgcc
     1321 gtaccaccag gtgagcaggt tccaggtgat ggcggccaac agacccgcca ggatcacggg
     1381 cagcgtgatg aactccggat ggaccgtctt gctgatggtg ttggccaccg catgatcggt
     1441 gaagatgaag aacgcgacga agttgaacac ggcggcccag agcacggcct ggaacggcgt
     1501 gagcaccttg gtgctgacga tggtggcgat ggcgttcgcc gcatcgtgga aaccgttgat
     1561 gtaatcgaac accaacgcca gcacgatgat ggtgatcagc agcggctcca tgccctcagg
     1621 cgtacttcag catgatggac tcgatcacat tggccacgtc ctcgcacttg tcggtggcca
     1681 gttccagtgt gctgtagata tcgcgccagc ggatcaggtc gatcgcgttc gtctccgtgg
     1741 cgaagaggtg cgcgatggcc ttgtcgtaca cgccgtcggc ctcgttctcc atgctgttga
     1801 tgtccaccac gaagcggatg ttcgtgttgg acttgtgcag atcacgcagg ctttgtacgg
     1861 ccagttgcac gatgcggcag ccctcgttca ccaggcgggc gagttcctgg caggtggcgt
     1921 cgggctgttc aatgccgaaa agttcgaggt tcttggcggc ggccaggatg aaatcggcca
     1981 catcgtccaa gctggtggcc aactgatgga tgtcctcccg atcgatcggt gtgatgaagt
     2041 tgcgcgccag gtcggtgaag atcgtgtgcg tcaggtcgtc gttggcgcgt tcctgcatct
     2101 ccaaccgctc caggatcgca tcgcgttggg agcctcgttt cgacgccatc atcaggatga
     2161 ggtcctcact catccgcagc acgttggccg cactcgcttc gaagtggtag aaaaaaatac
     2221 ggtccttcgg cttgaacaga cggaagaatg cttggaaggg catgatgacc gcgtcggatg
     2281 gtgatggcgg cgaaagtaga cggcccttcc gatagccagc gcggcaaggc gcagacttaa
     2341 cccaaggtta accttggcgg aaagccgggt gtagtttcgc acggatccgg gcccggcgcc
     2401 cgggtctccg aaccgctcat gctgtacgaa ttcaagggct accgtccggt ggtgcatcca
     2461 tcggctttcg ttcatcccca ggcggccgtc acgggcaacg tggtcattgg tgccgatgtg
     2521 tacataggcc cgggcgcggc gctgcggggc gattggggcg agatcgtgat caaggcagga
     2581 tgcaacgtgc aggagaactg cgtggtacac atgttccccg gagtgaaggt gaccttgcat
     2641 cccggtgcgc acatcggtca tggggccatc attcatgggg ccaccatcgg cgccaactgc
     2701 ttggtgggga tgaacgccgt ggtcatggac gacgtcgaac tgggcgaagg ctgcatcgtg
     2761 ggtgcattga gcttcgtgcc cgaagggagc aaatgggagg cgcgcaaggt gatcgtgggc
     2821 aacccggcgc gggtggtgaa ggacgtgagc gacgagatgc tcgcctggaa gaccgaaggc
     2881 acccgcctgt accaggccct gccggccgag ctgcaggcca ccttcggcca ccgggcccgc
     2941 gccctgtggg cggcggcgcc gcgcgagaag ctcgagcgca ccggcctcac cggctgggag
     3001 ctcgacgccc tgcccgaggt cgtcaccgtc gatgtcggcg ggcgcaagct gcaggccttc
     3061 ccggccctgg tcgatcgcga gaccgcggtc gacgtggtcc tgctcgagtc ggcggcggcg
     3121 gcgcgcgagg ccacccgcgc cggcctgcgt cgcctctacc tgctcggcat ggcgaccacg
     3181 ctgaccaagc tcgacggcca gttgccggcg gcgatcgcct ccgggccgct
//
LOCUS       DAOQOC010000208         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15162_length_3209_cov_3.493976, whole genome shotgun sequence.
ACCESSION   DAOQOC010000208 DAOQOC010000000
VERSION     DAOQOC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15162_length_3209_cov_3.493976"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2983
                     /locus_tag="PL106_04500"
     CDS             <1..2983
                     /locus_tag="PL106_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32357.1"
                     /translation="AQLGMLDIPFGTIPPHQTKVGQWWFTSSLLGKFVSYEASVVHAN
                     SFGNPELSLVQGVELHELTRSIREYGPLGDAMNDFLVNDQFDINDVPDIIYFSQGMRT
                     AEVLPAESGSFNAPVSAPTFTNTLTVTASEPGWNHIKLDDPGSGDYDIVSVTRNGDGQ
                     SIPLDNAWLTFVTLPVSQQPVYEDKFHFVDTLGNTSPTSYTVVWVPKNTDVPAVDTIM
                     GAPTMISATQVTQLTVVFDKPIDPATFTVDDLTLTFQGGPDLMSNAATITQVDPFTFT
                     VDLTALTTGDGLYVFTAQAAGVTDQFGISGVAGRQVSWTQFISVPAIVAFQGLPNGTT
                     DDEFTTIELLFNRPIDETSLTPADLTVLLGGSPQAGALTVARMNSDSTLFQVGGLENV
                     MNADGTYTLSVDMPNVLSTAGVPGLQAQSVALTLDGTGPTVVELTPITTGGLDAQHRT
                     GVNFRFSEPVSGLTIAAITITRDGNALPLLPAQLSQVDADEWRISNLGMASYPDGAYA
                     FTVSASGLSDAVGNVGSGSLATNWTVDRSTPITVTNVGISPDLGISSTDGLTSTLGFN
                     ALFTLSEPAAQVTIAQTSFGTENVLATLQNASAGPQSVPVSFPTGGNTGLKVTAIGSN
                     GGNASGTKNLYIDQTPLSATWQTANDQSLTADLTSATIQFSADLLDANLVDEAISFHK
                     DGMAQPTGALTITPVNGSTYTIGGLASVASGTGAYALSIDLSSLHKASSGIAGQGSAL
                     LNWTVVPTYTALLSARVFLEGPYANGVMRDSLRTLPSFPLTEPFTAMGYVHAASGGGE
                     TTTSSVLAIPGNDAIVDWVIVELRDADDNSDVVASRCALLQRDGDVVATDGLSPVNMA
                     VGAGSYHVAIRHRNHLGTMTEDTVTFASGVVNVDFTLLSTGLFGTDPQKQIGARRTLW
                     AGDVTFDNAVQYTGSGNDRDPILVAIGGFTPNNVVQGYLPADVNLDGRVKYTGPSNDR
                     DRILVNVGSNTPSNIRDGQLP"
     gene            3004..>3209
                     /locus_tag="PL106_04505"
     CDS             3004..>3209
                     /locus_tag="PL106_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32358.1"
                     /translation="MKPIFRNQLASGLLALFALPALAQPSVDIDLHRTDNGGIDVQLT
                     PDADFDGVPASLVFTVAWNAATTDA"
BASE COUNT          660 a         1110 c          908 g          531 t
ORIGIN      
        1 ggcgcaactg ggcatgctcg acatcccctt cggcactatt ccgccacacc agaccaaggt
       61 gggccagtgg tggttcacca gcagcctatt gggcaaattc gtgagctacg aggccagcgt
      121 ggtgcatgcc aacagcttcg gcaatcccga gctgagcctg gtgcagggcg tggagttgca
      181 tgagctcacc cgcagcatcc gcgagtacgg tccgctgggc gatgccatga acgacttcct
      241 ggtgaacgac cagttcgaca tcaacgacgt gccggacatc atctacttca gccagggcat
      301 gcgcacggcg gaggtgcttc ctgcggagag cggctcgttc aacgcaccgg taagtgcgcc
      361 caccttcacc aacacgctta ccgttacggc cagcgaaccg ggctggaacc acatcaagct
      421 ggatgacccc ggcagcggcg actacgacat cgtgagcgtg acgcgcaacg gcgacggcca
      481 aagcatccca ctggacaacg catggctcac cttcgtgacc ctgcccgtga gccagcaacc
      541 cgtatacgag gacaagttcc acttcgtgga caccctcggg aacacatctc ccaccagcta
      601 taccgtggtg tgggtaccga agaacaccga cgtgccggcc gtggacacca tcatgggcgc
      661 acccacgatg atctccgcaa cgcaggtcac gcagctcacc gtcgtcttcg acaagccgat
      721 cgatccggcc accttcaccg tggacgacct cacgctcacc ttccaaggag gacctgatct
      781 gatgagcaac gcggccacga tcacacaggt ggaccccttc accttcaccg tggacctcac
      841 cgcgctcacc accggcgatg gcctctacgt cttcacggcg caggctgccg gtgtcaccga
      901 ccaattcggc atcagcggtg tagccggccg acaggtctcg tggacgcagt tcatctccgt
      961 gccggcgatc gtcgccttcc aaggcttgcc caacggcacc accgacgatg aattcaccac
     1021 catcgaactg ctcttcaacc gaccgatcga cgagacgagc ctcacccctg cggacctcac
     1081 ggtgctgctg ggcggatcgc ctcaagcggg tgcgctcacc gtggcgcgca tgaacagcga
     1141 cagcacgctc ttccaagtgg gcgggctcga gaacgtgatg aacgcggatg gcacctatac
     1201 cttgagcgtg gacatgccga acgtgctgag caccgctggt gtgccgggcc tgcaagcgca
     1261 gagtgtggcg ctcaccctcg atggtaccgg acccacggtg gtcgaactca cgcccatcac
     1321 cacgggtggc ctcgatgcac aacaccgcac aggtgtgaac ttccgcttca gcgagccggt
     1381 ctcagggctg accatcgctg cgatcaccat cacacgggat ggcaacgcac tgccgctgct
     1441 gcccgcgcaa ctgtcgcagg tggatgccga cgagtggcgg atcagcaacc tgggcatggc
     1501 ctcctacccg gatggtgcct atgccttcac cgtaagcgcc tccggactct ccgatgccgt
     1561 gggcaacgtg ggaagcggca gcctcgcaac caattggacg gtcgatcgca gcacaccgat
     1621 caccgtgacc aacgtgggca tcagcccgga cctgggcatc tccagcacgg acggcctcac
     1681 cagtacgctc ggattcaatg cactcttcac gctcagcgaa ccggccgcgc aagtgaccat
     1741 cgcacagacc tccttcggca cggagaacgt gctggccacc ctgcagaatg ccagcgcggg
     1801 cccgcagagc gttcccgtga gcttccccac cggcggcaat acgggcctga aagtgacggc
     1861 catcggtagc aatggtggca acgcgagcgg gacgaagaac ctctacatcg accagacccc
     1921 gttgagcgct acatggcaaa cggcgaatga ccagtcactc acagcggacc tcaccagcgc
     1981 caccatccag ttctctgctg acctgctcga cgccaacctg gtcgatgaag cgatctcctt
     2041 ccacaaggac ggcatggcac aacccaccgg agcgctcacc atcacaccgg tgaacggaag
     2101 cacgtacacg atcggtggcc ttgcgtccgt ggccagcgga accggtgcct atgcgctctc
     2161 gatcgacctc tcgtccctcc acaaagcgag cagcggcatc gccggacaag gatccgctct
     2221 gctcaattgg accgtggtgc cgacatacac cgcacttctc agtgcccgcg tgttcctgga
     2281 gggcccgtac gcgaacggcg ttatgcgcga ctccttgcgc acactcccga gcttcccact
     2341 cacagaaccc ttcacggcca tgggttacgt ccatgcggcc agcggaggtg gtgaaaccac
     2401 cacctcatcc gtactcgcca ttcccggcaa cgatgcgatc gtggactggg tgattgtgga
     2461 actcagggat gccgacgaca acagcgatgt ggtggccagc cgttgcgcac tcctgcaacg
     2521 cgatggtgat gtggtggcca cggacggcct ttcaccggtg aacatggccg ttggcgcggg
     2581 cagttaccat gtggccatcc gccaccggaa ccacttgggc accatgacgg aggacaccgt
     2641 caccttcgcc agcggagtgg tgaatgtgga cttcaccctg ctctccaccg gactcttcgg
     2701 gacggacccg cagaaacaga tcggtgcgcg tcgcacatta tgggccgggg atgtcacctt
     2761 cgacaacgcc gtgcagtaca ccggctccgg aaacgatcga gacccgatcc tggtggccat
     2821 cggcggcttc acaccgaaca acgtggtcca gggctatctc cctgcggatg tgaacctcga
     2881 cggccgcgtg aaatacacgg gaccctccaa cgaccgcgac cgtatcctcg tgaacgtagg
     2941 aagcaacaca cccagcaaca tccgtgacgg tcaactgcct tgaacctcct catccgaacg
     3001 atcatgaaac cgatcttccg caaccaactc gcatcaggac tgctcgccct gttcgccctt
     3061 cctgcgctcg cccaaccatc ggtggatatc gacctgcacc gcaccgacaa cggggggatc
     3121 gatgtgcaac tcacaccgga tgcggacttc gacggtgtgc cggcctccct ggtcttcact
     3181 gtcgcctgga acgcggccac caccgatgc
//
LOCUS       DAOQOC010000209         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15213_length_3203_cov_3.585451, whole genome shotgun sequence.
ACCESSION   DAOQOC010000209 DAOQOC010000000
VERSION     DAOQOC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15213_length_3203_cov_3.585451"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1393)
                     /locus_tag="PL106_04510"
     CDS             complement(206..1393)
                     /locus_tag="PL106_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32359.1"
                     /translation="MNRSYPILFVASTLLTACGQSEPEMNDPYAGHMSDLEARVSSIT
                     GQQYAQDGSGMEGEAGYEGAGDQSYANQADQGGTRTLTIHNSQFNVPMAHIELPTRWP
                     VKSTPGGDWSVESPDLKVKAMQGATFVYAGGQYGQFYQQGGGQMRAPLAPDQVVMQDL
                     VPNMQRQGGELIGVTDLPAVARADQQGLDGLYSIGQVRKTCRAALSEWRKGDQRIALV
                     LHWFAFEGSDMTNWGYRITGMQASPRNYEQDKAALVNSLSSVRWDRAYFAAYAQSEQQ
                     KEGQSWATHNQRMASNQAAFDAQQRTHRETWGAINDASMGAYNSRMQSMDRMQNATIN
                     GIRGEQDAMNPYTGQQGKVEAGYQNYWVNRDGQYFGTNDGMYDPNANSDQVDQWQQMP
                     TEP"
     gene            complement(1628..2206)
                     /locus_tag="PL106_04515"
     CDS             complement(1628..2206)
                     /locus_tag="PL106_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNA32360.1"
                     /translation="MNPKVDWFFAKNDPWQDCYARLREIAQESRLTEELKWGHPCYTL
                     NGKNVFLIHGFNDYCALLFHKGALLKDDHCLLVQQTENVQSARQIRFTSAKQIDKLTP
                     VVRGYIQQAIEVERSGMKVLLKKTAEFDMPDEFKRALKDMPALKKAFNALTPGRQRGY
                     LLHFSSAKQAKTREARIDKNLERILAGKGLED"
     gene            complement(2221..2646)
                     /locus_tag="PL106_04520"
     CDS             complement(2221..2646)
                     /locus_tag="PL106_04520"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA32361.1"
                     /translation="MSTATSKPVKLLSGGNPQIAKGDGDAPVQAYIAAMPGWKREVGE
                     RLDALIERHIPRLRKAVKWNSPFYGVEGQGWFLSTHVFTHFVRLTWFQGRSLKPIPPG
                     PSKDKNARYLDIRENGIEDEKLLISWLKQAAKLPGWLGH"
     gene            complement(2983..>3203)
                     /locus_tag="PL106_04525"
     CDS             complement(2983..>3203)
                     /locus_tag="PL106_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HNA32362.1"
                     /translation="FSHALGSGKGAAGLLVSDTAFAADMRRTITRLDTGSLLLNENLR
                     ALKSNWFFRGYFKKQEKELKKQEKAGKP"
BASE COUNT          528 a          977 c          951 g          747 t
ORIGIN      
        1 tgagccgagg ggaaagatct gaacgctggt gatggaagtg aaggagatga ggatcgcaat
       61 ggaaaccaca gtctccctga cctccttctc ctcccatacc cgtgtcacat ggcgatgatc
      121 gaccgcttct ttgtcactgt cccagaaaag gcaacaggac cccgaagtga ggtcctgttg
      181 ccatgatgat cgtttttcgt gttgactacg gctcggtggg catctgctgc cactggtcca
      241 cctggtcgct gttggcgttc gggtcgtaca tcccgtcgtt ggtgccgaag tactggccat
      301 ccctgttcac ccagtagttc tggtatccgg cctccacctt gccctgctgt ccggtgtagg
      361 ggttcatggc gtcctgttcg ccgcggatcc cgttgatggt ggcgttctgc atgcggtcca
      421 tgctctgcat acggctgttg tacgcgccca tactggcgtc gttgatggcg ccccaggtct
      481 cccggtgcgt gcgttgctgg gcatcgaagg ccgcttggtt gctggccatg cgctggttgt
      541 gcgttgccca gctctggcct tccttctgct gttcgctttg ggcatatgcg gcgaagtagg
      601 cgcggtccca gcgaacactc gacagactgt tcaccagggc tgccttgtcc tgctcgtagt
      661 tccgcggcga ggcttgcatc ccggttatcc ggtagcccca gttcgtcatg tccgaaccct
      721 cgaaggcgaa ccaatgcagc accagtgcga tgcgctggtc gcctttgcgc cattcgctca
      781 gtgccgcccg gcaggtcttg cgcacctggc cgatggagta gagcccatcg agtccttgct
      841 gatcggcacg ggccactgcg ggcaggtcgg taacaccgat caactccccg ccctggcgct
      901 gcatgttggg cacaaggtcc tgcatcacta cctggtccgg agccagaggt gcgcgcatct
      961 gcccaccacc ttgctgatag aactggccgt actggcctcc agcgtacacg aaggtggcac
     1021 cttgcatggc cttcaccttc aggtccggcg actccacgct ccagtcacct ccaggtgtgg
     1081 acttcacggg ccagcgggtg ggtagttcga tgtgcgccat gggcacgttg aactggctgt
     1141 tatggatggt cagcgtccgc gtcccacctt ggtcagcttg gttggcatac gactggtcgc
     1201 ccgccccctc gtatccggct tcgccctcca tgcccgatcc atcctgggca tactgctgac
     1261 cggtgatgga ggacacacga gcttccaggt cgctcatgtg cccggcatac gggtcgttca
     1321 tttcaggttc gctctgacca caggcagtaa gcagggtgct ggcgacgaac aggataggat
     1381 agcttcggtt cattcgtgtt tgattttaca ctctatcggg atggggcggc ggatgtgaac
     1441 agtgcggtaa aaaatcttcc ggcaggcgca attcccgcca gggtagccat agtaccggtc
     1501 ggactgttga gcgcgcgtgc gaaccggccc acctgttggc ccaccgcatg gaggaagtca
     1561 ctgttctgcg ccaggtttgg cgcacatggg ccgcacttgt gcttcagcct gggctgaggc
     1621 cgtaacgtta atcctccagc ccctttccgg ccaggatccg ctccaggttc ttatcaatgc
     1681 gtgcctcacg ggtcttcgcc tgcttggcgg atgagaagtg gagcaggtag ccgcgttgcc
     1741 tgcccggcgt gagggcgttg aatgccttct tcaacgcagg catgtccttc agggctctct
     1801 tgaactcgtc cggcatgtcg aactcggcgg tcttcttcag gagcactttc atgcccgacc
     1861 gttccacctc gatggcctgt tggatgtatc cccgtacgac aggcgtcagc ttatcgatct
     1921 gcttcgcgct ggtgaatcgg atctggcgcg cgctctgcac attttcggtc tgctgtacca
     1981 gcaggcagtg atcgtccttc agcaaggcac ccttgtggaa gagcagggca cagtagtcgt
     2041 tgaagccgtg gatcaggaag acgttcttgc cgttgagcgt gtaacaaggg tggccccatt
     2101 tcagctcctc tgtgaggcga ctttcctggg ctatctcacg cagccgggca tagcagtcct
     2161 gccaggggtc attcttcgca aagaaccagt cgactttcgg gttcatcgtg gccttcggtg
     2221 tcaatgcccc aaccatccgg gcagcttcgc cgcctgcttc agccagctga tcagtagttt
     2281 ttcatcctcg atgccgttct cgcggatgtc caggtaacgc gcgttcttgt ccttgctcgg
     2341 tccgggcggt atcggcttca acgaccggcc ttggaaccag gtgagccgaa cgaaatgggt
     2401 gaagacatgc gtggacagga accacccctg accctcgacc ccgtagaacg gcgagttcca
     2461 cttcaccgct tttcgcaggc gggggatgtg acgttcgatc aacgcatcga gccgttcgcc
     2521 cacttcgcgc ttccagcccg gcatcgcggc gatataggcc tgcaccggcg catcaccatc
     2581 gcccttcgcg atctgcgggt tgccgccgga gaggagtttt acgggtttgg atgttgcggt
     2641 ggacatgttc caaaagtagg gtagtgggcc atgctattgg tcagcatgcc ccggattgga
     2701 cctgctatgg acgcggatca gccgcgtcca tagcaggtcc atttgtatcc atggaaagtg
     2761 cagccaaacg gccgtctgca ccacccccat gttgagcgct ctcgtcgccg ggctctgctg
     2821 cgccgctata agactccgaa gtgtcttcgc gtaggtggct tcgatgccca tgttgatcca
     2881 cgttcgcata tccaacggat tgatgatcgc atccagccac aaccttgcag ccgcgtaata
     2941 cgggctggtg ctttcgatgt acttcgcgcg gggatgcccc gatcaaggtt ttccggcctt
     3001 ctcctgcttc ttcagctcct tttcctgctt cttgaaatag ccgcggaaga accagttgct
     3061 cttcagggcg cgcaggttct cgttcagcag gaggctgccg gtatccaagc gggtgatggt
     3121 gcggcgcatg tccgccgcga atgccgtgtc gctcaccaac aggccggcgg ctcctttccc
     3181 gcttcccaat gcgtggctga agc
//
LOCUS       DAOQOC010000210         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15257_length_3198_cov_3.466115, whole genome shotgun sequence.
ACCESSION   DAOQOC010000210 DAOQOC010000000
VERSION     DAOQOC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15257_length_3198_cov_3.466115"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            494..619
                     /locus_tag="PL106_04530"
     CDS             494..619
                     /locus_tag="PL106_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32363.1"
                     /translation="MSEQWNHTGRPAASGIDHGLTGQNGMPLHHPTIEHGSSIAR"
     gene            666..1223
                     /locus_tag="PL106_04535"
     CDS             666..1223
                     /locus_tag="PL106_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA32364.1"
                     /translation="MAKKTREHHGAGRLPVRVVPTGQEGSVPIPSNIDSNAARALLPS
                     GLEHALPGQQATPPPSPSEEATPTEPVFIREGNGLVRIDLVDLRFARADGNHVELHFG
                     STHHVLRSSLSQVLRSLPTGTLVQVNRSHAVNIQHIVHVGTDQVALDDQVFTVSHIYR
                     EDLLRHLRIISDRSNALLSPRRRRV"
     gene            1347..2132
                     /locus_tag="PL106_04540"
     CDS             1347..2132
                     /locus_tag="PL106_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32365.1"
                     /translation="MIRDLRKTLMGTALLLGATFAQAQTHQDPVDIGLFQNGSELEVR
                     LRPSADFDGVVSAVVFTLRWETAGGGAISTLTQRGAEENYIPSRPSGGLHEDGPFTYQ
                     IYAGFGMEAMADRGTRWVAGKEYVVATIPVSGGSEFVLADDAWTKRDENNGAFYVSLG
                     GVDHTGVIYKGVAGSTNATPFSITPNPSRGLFNLVIPVGEGENVSYEVLNSAGQTVAK
                     KQLNVEGGGVYREEMDLTSSGMGTYHLRVLRNNTVETHKLVVH"
     gene            2455..2838
                     /locus_tag="PL106_04545"
     CDS             2455..2838
                     /locus_tag="PL106_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA32366.1"
                     /translation="MLIPLRAPHQKVDTRADTARTQDAVFIRDRHGLQRVERNAINLL
                     RAEGNYVEVHTRDRRFVLRNSLRETITQLGEERFIQVNRHTAVNIGHLERVDCDSLEL
                     DGQCITLSRCYRENLMSHLNILCCR"
BASE COUNT          659 a         1004 c          921 g          614 t
ORIGIN      
        1 cctgaaagtg ctgaggatcg cttgttcagg aacgattccg gcttgttgga gcgctgcggc
       61 gagctcggcg tgacagatca tagggagtgt tcacgaacat tgctcctcga cacgcttgtc
      121 ccccactgtc acgctgagcg cgccgaagcg ccatacgcct gttattcatg gtcacccggg
      181 atcctggtgc gatcccgcgc cacaagccct gatggacgcg cacttctgtt ccgggccgag
      241 tggcccgatc gccgggcaca ggaacaggac ggtccactgc cggtatgccg acgaagatcc
      301 attacaacaa ccacggaaaa aattcaatcc ccatttcagg ggatgagcct ggaccgtatg
      361 gatcgggaac cgcgtgggtc cttgaaaatt cgtcgatacg tacacggcct tgggcgaaaa
      421 tccatgatgg tccatcgcct caagatccgc atatcatccg atcctttcta agttgcaata
      481 gcgtaggaaa gccatgagcg aacagtggaa ccatacgggc cgacccgcgg cgagcgggat
      541 cgatcacggc ctcaccgggc agaacggtat gccgctgcac cacccgacga tcgagcacgg
      601 gtcctccatt gcacgttagg caccccaagc gacgacccta cacccgcgaa gagcacggcc
      661 tccgcatggc gaagaaaaca cgtgaacacc acggggcagg ccgcctcccc gtgcgcgtgg
      721 tgcccactgg gcaggagggt agcgtaccta taccctccaa catcgactcc aatgcggcgc
      781 gtgcgttgct cccatccggg ctcgagcacg ccctacccgg gcaacaggct actcccccac
      841 cttctccctc cgaagaagca acacccactg agccggtttt cattcgggaa gggaacggcc
      901 tggtccggat cgacctggtg gatcttcggt tcgcccgggc cgacggcaac cacgtggaac
      961 tccacttcgg ttccacccac cacgttcttc gttcgtcgct gtcccaagtg ctccggtccc
     1021 tgcccacggg cacactggtc caggtgaacc gatcccatgc cgtcaacatt cagcacatcg
     1081 tgcatgtggg cacggaccag gtggcactgg acgatcaggt cttcacggtc tcgcacatct
     1141 acagggaaga tctcctgcgt cacctgcgga tcatctccga tcgttcgaat gcactgcttt
     1201 caccgcgccg gcgacgcgtt tagtgtacgg cattcgccat ttcgtgtaca atatcgtccg
     1261 ggtccgggac cgatcccgca atttcacatg cccgctccga ggcgggcagc gtcctatccc
     1321 atcctccacc gacaagcaca ccgaccatga tacgcgactt gagaaagacc ctgatgggca
     1381 cggcactcct gctgggagcg accttcgcac aggcacagac acaccaggac cccgttgata
     1441 tcgggctgtt ccagaacggc agtgaattgg aggtgcgctt gcgtccctcg gctgattttg
     1501 acggggtggt gagcgcggtg gtattcaccc ttcgctggga aacggccggt ggcggtgcca
     1561 tcagcaccct cacgcaacgg ggtgccgagg agaactacat cccctcgcgc ccgtccggtg
     1621 ggctgcacga ggatgggcca ttcacctacc agatctacgc gggcttcggc atggaagcca
     1681 tggctgatcg cggaacacgc tgggtggccg gcaaagagta cgtcgttgcc acaattcccg
     1741 tgtccggcgg tagtgaattc gtccttgccg atgacgcctg gacgaagcgc gacgagaaca
     1801 atggtgcgtt ctatgtgagc ttgggaggtg tggaccacac cggtgtgatc tacaaagggg
     1861 tagccggttc caccaacgcc acccccttct ccatcacccc gaatcccagc cggggtctct
     1921 tcaacctggt gatccctgtg ggcgagggcg agaacgtctc gtacgaggtg ttgaattccg
     1981 ccggccaaac ggtggccaag aagcagctga acgtggaagg cggcggtgtg taccgcgaag
     2041 aaatggacct caccagttcc ggcatgggca cctaccacct gcgcgtgttg cgcaacaaca
     2101 ccgtggagac gcacaagctc gtggtgcatt gaccccatcg agcggatcct gcgccctgag
     2161 catgtcatga acggaccggt ccgtaggggg gccggtccgt tcatgtggac ccgaatccga
     2221 ggtctaccgc acctgcaacg agcgctatcg ccccatgaaa aaaacaaaat ttactgacag
     2281 ccgtcagtgc gaatctcaaa agaattcata ctttagccgg accctggtgc tccacaaagg
     2341 ggaaactgac atgcacgcca aaccctttcg accatggctg atgaggtctg tactggagca
     2401 caagatcgat cctttgatgt tgtgatcgct cgtgatgcgg gatcccatga tcccgtgctc
     2461 attccccttc gtgccccgca ccagaaggtg gatactcggg ctgatacggc ccggacacaa
     2521 gacgcggtct tcatccgcga ccgccacggc ttgcagcggg tggagcgcaa cgccatcaac
     2581 ctgctgcgtg ccgagggcaa ctatgtggaa gtgcataccc gggatcggcg cttcgtgctg
     2641 cgcaattcgc ttcgcgaaac gatcacccag ttgggcgagg agcgcttcat ccaggtcaat
     2701 cgccacaccg cggtcaacat cggtcatttg gagcgcgtgg attgcgacag cctcgaactc
     2761 gatggccagt gcatcacact ctcacggtgc taccgcgaga acctgatgag ccacctgaat
     2821 atactgtgct gtaggtagat cagggcctgc gaccggcacg gtaccgcggc attcacgtcg
     2881 cggaggaccg atcgacctac gtcgtaattc ctgttgaatg tcacggtgag tccgtcgaag
     2941 cgccgatctc ctgttctcag cggtcgcccc ggatcttcgg ttcgatcccg ccccgcacgc
     3001 cttgatggac aggcgcattt gtttttggtc gggtgaatcg cccttcattc ctcatttccg
     3061 atcaaggaaa tgctcagagc ggcttgaaag tgctgatgat cgcgtattca gaaacgatac
     3121 cggccggttg tagcgcttct cgcatcgccg gagcggctac gcataagcgc agtcggcggg
     3181 ctcagcgtga cagttcat
//
LOCUS       DAOQOC010000211         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15291_length_3195_cov_3.291401, whole genome shotgun sequence.
ACCESSION   DAOQOC010000211 DAOQOC010000000
VERSION     DAOQOC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15291_length_3195_cov_3.291401"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1272)
                     /locus_tag="PL106_04550"
     CDS             complement(88..1272)
                     /locus_tag="PL106_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA32367.1"
                     /translation="MEQAADPVFSRPFSPERMISVSNLSLHFGERTMFDAVSFFIGKD
                     DRIGLVGRNGAGKSTLLKMLAGEDKPDSGSIDKPRDLTVGYLPQTMKHDLSLSPRQIA
                     MSAFKEAEWLEARISAIENELNASFEDEARMMALAEELAHAHERLNHIGAGDHDKQVE
                     ETLRGIGYAEADMDRPLMELSGGWRMRAELCRILLMSPDLLLLDEPTNHLDLPAIQWL
                     EDLLMTYPAALVLISHDKTFLDRLTRRTIEVGGGRLIDRKVPWSQFVELRKEEKARQM
                     KAYEEQQKYIEHTQELINKFRAKKDKAAFAQSLITKLEKLDRVIVEDDAFRSMIVKFP
                     PAPHSGKLVLEMHGAKKSYGERVILTDAELTLARGEAIALVGANGTGKSTVMRMLVVE
                     GV"
     gene            complement(1347..2207)
                     /gene="gldN"
                     /locus_tag="PL106_04555"
     CDS             complement(1347..2207)
                     /gene="gldN"
                     /locus_tag="PL106_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNA32368.1"
                     /translation="MNNRGTSLIAACAALVLGLPVSSDAQTVLDGAYIKEHTKTQRVV
                     PYPFIREADVMWYRRIWREIDVTEKINQPLYYPLEKINDRKSLFDVIKDGLLVDGSIT
                     AYDPGADGWEDEFKRPLLTAEIKELLSRTDSVWVDRLDGTGQDLNVVTVETKSENVKR
                     YRLKEDWIFDKQRSSMDIRIIGLAPLKEKLDQNDGSLRGYSVLFWLYFPELRYVLVNW
                     EVFNRQNDAERRSFDHVFWTRQFNSTIIKESNVYNRVLADVKTGINGVLESESIKQDL
                     FEFEHDLWNF"
     gene            complement(2235..>3195)
                     /locus_tag="PL106_04560"
     CDS             complement(2235..>3195)
                     /locus_tag="PL106_04560"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HNA32369.1"
                     /translation="EMVAKLLGNVEKTSFKFNTLDAIVKPQSSYVTTGSKYRAEIFLG
                     AYDNQNAPEVYICGPGAKVDTSAKVPSIIGESIKLPMEGAKAILEQVAAGAGLRQVSG
                     IIKFTPVGGEPVVQPFTTEYEVAAPNLVVSPTKMNVFYRGIDNPVDISVAGYSAKDIS
                     PSMNNGSLTKAAQGYVVRPGSGPDATVGVSVTNPDGTKKSLPGVQFRVKDIPKPQPFF
                     GGKTVTDQSIKKTELTASAGVIAKLENFQFDLTFQIVEYTVSATLSGSLAEERVTGAA
                     MTSKAKEIVGKVKTGQKVYIENIKAKGPDGRTQSIGTLAFKVM"
BASE COUNT          544 a          961 c          987 g          703 t
ORIGIN      
        1 ggttgccgcg gctgtcgagg atctcgcggc cgacgatgtc gacgatggca ctcattgcgg
       61 tttctcttca aaaagttggt aaacggttca gacaccctcg accacgagca tgcgcatcac
      121 ggtacttttc ccggtaccgt tggcacccac cagcgcgatg gcctctcccc gcgcaagggt
      181 gagttccgca tcggtgagga tcacgcgttc gccgtagctt tttttcgccc cgtgcatctc
      241 gagcacgagc ttgccactgt gcggtgcggg ggggaacttc acgatcatcg atcggaacgc
      301 gtcatcctcc acgatcacgc gatccagctt ctccaacttg gtgatcaggc tctgggcgaa
      361 cgcggccttg tccttcttcg cgcggaactt gttgatcagt tcctgggtat gttcgatgta
      421 cttctgctgc tcttcgtaag ccttcatctg gcgcgccttc tcctccttgc gaagctccac
      481 gaactggctc cagggcactt tgcggtcgat gaggcggccg ccgcccacct cgatggtgcg
      541 acgggtaagg cgatcgagaa aagtcttgtc gtggctgatg agcacgagcg ctgccgggta
      601 ggtcatgagc aggtcctcga gccactggat ggccggaagg tccaggtggt tggtcggttc
      661 gtccagcagc aggaggtcgg ggctcatgag caggatgcgg cagagctccg cgcgcatgcg
      721 ccagccgccg ctcaactcca tcagagggcg gtccatatcg gcttcggcat acccgatacc
      781 gcgcagcgtt tcctccacct gcttgtcgtg gtcgccggca ccgatgtggt tcaggcgctc
      841 gtgggcgtgc gcgagttcct cggccagggc catcatcctg gcctcatcct cgaaagaagc
      901 attgagctcg ttctcgatcg cggagatgcg cgcttcgagc cattcggcct ccttgaaggc
      961 gctcatggcg atctgtcggg ggctgagcga caggtcgtgc ttcatggtct ggggcagata
     1021 gcccacggtg aggtcgcgcg gcttgtcaat gcttccactg tcgggcttgt cctccccggc
     1081 gagcattttg agcagtgtgc tcttgccggc gccgttgcgg cccaccaggc cgatccggtc
     1141 gtccttgccg atgaagaagg acaccgcgtc gaacatggta cgctcgccga aatggaggct
     1201 gaggttgctg acactgatca tgcgttccgg ggaaaagggg cgcgaaaata ccgggtccgc
     1261 ggcctgttcc atccggaggg gcggggctcc gacaaagaag aaaccccgac caggggccgg
     1321 ggttcccatg acatgcgggg atccactcag aagttccaga ggtcgtgctc gaactcgaag
     1381 aggtcctgct tgatggactc gctttcgagc acgccgttga tgcccgtctt cacatcggcc
     1441 agcacgcggt tgtacacgtt gctctccttg atgatggtgc tgttgaactg gcgcgtccag
     1501 aagacatggt cgaaggacct ccgctccgca tcgttctgac ggttgaatac ctcccaattc
     1561 accagtacat agcgcagttc ggggaagtag agccagaaca gcacggaata gccccggagc
     1621 gagccgtcgt tctgatcgag cttctccttc agaggggcca gtccgatgat gcggatgtcc
     1681 atggaggacc gctgcttgtc gaagatccag tcctccttca ggcggtaccg cttcacgttc
     1741 tcgctcttgg tctccacggt caccacgttc agatcctggc cggtgccgtc cagccggtcc
     1801 acccagaccg agtccgtgcg cgaaagcagc tccttgatct cggcggtgag cagcgggcgc
     1861 ttgaactcat cctcccagcc gtcagccccg ggatcatatg cggtgatcga accatcaacg
     1921 agcaggccat ccttgatgac atcgaagagg ctcttgcgat cattgatctt ctccagcggg
     1981 tagtacagtg gctggttgat cttctcggtc acatcgatct cgcgccagat ccggcggtac
     2041 cacatcacat cggcttcgcg gatgaagggg tagggaacca cccgctgggt cttcgtgtgt
     2101 tccttgatgt acgcgccatc cagcacggtc tgtgcatccg atgataccgg caggcccagg
     2161 acgagcgcgg cgcaggcggc gatcagagag gttccacggt tgttcatgat gcttgctggt
     2221 tgatgcgtac gggatcacat gaccttgaac gcgagcgtcc cgatgctctg cgtacggcca
     2281 tcgggccctt tggctttgat gttctcgatg tagaccttct gtccggtctt caccttgccc
     2341 acgatctcct tggccttgga cgtcatggcg gcgccggtca cacgctcttc ggccaagctg
     2401 ccggaaaggg tggcggacac ggtgtactcc acgatctgga aggtgaggtc gaactggaaa
     2461 ttctccagtt tggcgatcac cccggccgat gcggtgagtt cggtcttctt gatgctctgg
     2521 tccgtaacgg tcttgcctcc gaagaagggt tgcggtttcg ggatgtcctt cacacggaac
     2581 tggacacccg gcaacgactt cttggtccca tcagggttcg taacgctgac accaacggtg
     2641 gcgtcggggc ctgagcccgg gcgcaccacg tacccttgcg cggccttggt gagtgatccg
     2701 ttgttcatgg acggggagat gtccttggcg ctgtagccgg ccacgctgat gtccacagga
     2761 ttatcgatgc cgcggtagaa cacgttcatc ttggtggggc tgaccacgag gttcggcgcg
     2821 gccacttcgt actcggtagt gaagggctgg accacgggtt cgccaccgac cggggtgaac
     2881 ttgatgatcc cggagacctg ccgcaggccg gcaccggccg caacctgctc gaggatggct
     2941 ttggcgcctt ccatgggcaa cttgatgctc tcgccgatga tcgatggcac tttggcgctc
     3001 gtatccacct tggctcccgg gccgcagatg tacacctccg gcgcgttctg gttgtcatac
     3061 gcgccgagga agatctccgc gcggtatttg ctacccgttg ttacgtagct gctctggggc
     3121 ttgacgatgg catcaagcgt attgaacttg aagctggtct tctccacgtt gcccagcagc
     3181 ttggccacca tttcg
//
LOCUS       DAOQOC010000212         3176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15456_length_3176_cov_3.644024, whole genome shotgun sequence.
ACCESSION   DAOQOC010000212 DAOQOC010000000
VERSION     DAOQOC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15456_length_3176_cov_3.644024"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(385..564)
                     /locus_tag="PL106_04565"
     CDS             complement(385..564)
                     /locus_tag="PL106_04565"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNA32370.1"
                     /translation="MKSTGNGVLVNIILGLIGGVVGSLVFGVVGLQAYGTLGNLVMAT
                     VGAVVLIVLARAIRN"
     gene            complement(702..1070)
                     /gene="rplS"
                     /locus_tag="PL106_04570"
     CDS             complement(702..1070)
                     /gene="rplS"
                     /locus_tag="PL106_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNA32371.1"
                     /translation="MDKIKQLEKEWAPVKQYPDFKAGDTITVSYKIVEGNKERIQQFQ
                     GVVIQRKGSGSTATFTVRKMSGNVGVERIFPIASPFIDAIALNKRGDVRRARIFYLRE
                     RRGKSARIQEKRQLVTEEAK"
     gene            complement(1439..2119)
                     /gene="trmD"
                     /locus_tag="PL106_04575"
     CDS             complement(1439..2119)
                     /gene="trmD"
                     /locus_tag="PL106_04575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNA32372.1"
                     /translation="MRIDILSAVPRLLDSWLNESILERAQKKGLAEIRVHDLRDHSTD
                     KHRRLDDYAFGGGAGMVMMIEPIDRCITWLKSERSYDEVIYMSPDGELLDQPLANRMS
                     AHRNIIILCGHYKGVDERVREHLITREISIGNYVLSGGEIPAAVLTDALIRLQPGVLN
                     DETSALSDSFQDGLVAPPVYTRPAEYNRWSVPEVLLSGHEARIHAWRHEQSLERTRAR
                     RPDMAEGE"
     gene            2289..3014
                     /locus_tag="PL106_04580"
     CDS             2289..3014
                     /locus_tag="PL106_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32373.1"
                     /translation="MMRSYVLAAIAFLPCLAQAQKQPYEDLLVRYVDEKYEDCLGKAF
                     MYTEKESTKKDPLPFLYVSMCYYEMSKIDKYRAMEEYKKADRDAVKYAEKYRKKDKNK
                     EFFDNYADFWSELNIMAQDAGLTAYEDKQYAKAKQWFDGMVGYYPENPGAHLMLGLAL
                     LRTNMVKEGDAAIAKYQPALEAAGGAGLLPDDQKKLLKSAVIHYAEYLDTKGNRSKAK
                     EVLEAVKEFYADDAEFKGALESY"
BASE COUNT          663 a          921 c          953 g          639 t
ORIGIN      
        1 ggcgccggta ttatccccgg tgaagttgat cgaaccgccg atggggagca ccacttcgga
       61 cacggttgaa cattcatcgt tgatcggggc atcggccgcg cggttccata ccggtttcag
      121 cgtgccagga tattccaggg gccaggacca caaccggtgg tgtctgcgcg tggatcaaac
      181 cgcccgtgag gaaggcgaag aaggtgcaca agagatggtt ccgggtgttc atggtgttgg
      241 tgaggctggc gatccgtgag gggccgaaag gtggggaaga agaccgtaac atgaatgtgt
      301 cgcgcactga cccgatccgg tacgggtaga ggccgatccc cgatccgtga tgtgtgttgc
      361 cgggtaccgg accgcttgcg ttggtcaatt gcggatcgca cgggcgagca caatgagcac
      421 gaccgcaccg accgtggcca tgaccaggtt accaagggtg ccgtacgcct ggaggccgac
      481 caccccgaag accaggctgc cgacaacccc tccgatcaac cccaggatga tgttgaccag
      541 caccccattg ccggtacttt tcatgatccg tcccgcgagc cagccggcca cgcctcccac
      601 gatcagactc catatcagac ccatgccgaa aggtagcgat cgccgttgaa tgacgaaggc
      661 ccctttgcag gagccttcgg aacgatggat ccgatgcggc cctatttggc ctcttcggta
      721 accagttggc gcttctcctg gatacgtgcg ctcttgccgc ggcgctcgcg caggtaaaag
      781 atccgtgccc gacgcacatc gccacgcttg ttcagcgcga tcgcgtcgat gaacggggag
      841 gcgatcggga agatccgttc cacacccacg ttgccgctca tcttgcgaac ggtgaacgtg
      901 gccgtgctgc cgctgccttt gcgctggatc actacgcctt ggaactgttg gatgcgctcc
      961 ttgttaccct cgacgatctt gtagctgacg gtgatggtat cgcctgcctt gaagtcagga
     1021 tactgtttca caggggccca ctctttctcg agttgtttga tcttgtccat gactttcagc
     1081 ggcttatgcg ggagggttcc gcgtttgggg gcgcaatgtt aggcaaaatt catgggttgg
     1141 cgtgaatgat cagaagagaa agatgctgag gtggtgaagt gcatgaggct tgggccaggg
     1201 ctgtggtggc ggagacgcag aattgacaat ttgcttggct ggccaaacaa gcattttgag
     1261 ctgcggcagg ggggagcttg gccctgggta acgctgacaa attgagctca gtgtgccgtc
     1321 cttgctgaag aatttgtcga tttcaacgga ttgcggatgt ttgcttggcc agccaagcaa
     1381 atgggccgca catttggaag aacgcttgcc tgaacaagac cattgcgacc ttccgggtct
     1441 actccccttc ggccatgtcc ggtcgtctgg cccgggtgcg ttccaaactc tgttcatgcc
     1501 tccaagcatg tatccgtgct tcgtgcccgc tcaacaatac ctccggaacg ctccacctgt
     1561 tgtattccgc gggccgcgtg tacacaggtg gtgccaccag gccgtcctgg aagctgtcgc
     1621 tcaatgccga ggtctcatcg ttcaacacgc caggttgcaa tcggatgagt gcatccgtca
     1681 atacagccgc ggggatctcc ccaccgctga gcacataatt gccgatcgag atctcgcgcg
     1741 tgatcaggtg ttcgcgcacg cgttcgtcca cgcctttgta atgcccgcac agaatgatga
     1801 tgttcctgtg cgcgctcatc cggttggcca gcggttggtc caggagttcc ccatccggac
     1861 tcatgtagat gacctcgtca taggaccgct cggacttcag ccaggtgatg cagcgatcga
     1921 tcggctcgat catcatcacc atgcccgcac cgccaccaaa ggcgtaatcg tccagccgac
     1981 gatgcttgtc cgtgctgtgg tcgcgcaggt cgtgtacgcg gatctccgcc agccccttct
     2041 tctgggcgcg ctccaggatg ctctcgttga gccagctgtc cagcaaccgt ggaacggcag
     2101 agaggatgtc gatccgcatc atgcggcaaa ggtgggaaac gggtcggtac ccggcaggag
     2161 gtgtccgttc ctggtccccg accaacatcg ccgcactgac aacctgtaca cgcagggccg
     2221 cccggatcgg accttggtcc tattttcggc atcgttcttg gaaatccacc tcatcaaacc
     2281 cacgcaccat gatgcgttcg tacgttcttg cagccatcgc tttcctgccc tgcctcgccc
     2341 aagcccagaa acagccgtac gaggatctgc tcgtccgcta cgtggatgag aaatacgagg
     2401 actgcctcgg caaggcattc atgtatacgg agaaggaaag cacgaagaag gacccgttgc
     2461 ccttcctcta cgtgagcatg tgctactacg agatgagcaa gatcgacaag taccgcgcca
     2521 tggaagagta caagaaggct gatcgcgacg cggtgaagta cgctgaaaaa taccggaaaa
     2581 aagacaagaa caaggagttc ttcgacaact acgccgattt ctggagcgaa ctcaacatca
     2641 tggcccagga tgcgggcctc acggcctatg aggacaagca gtacgccaag gccaaacaat
     2701 ggttcgatgg catggtcggg tactatccgg aaaatcctgg tgcccacctc atgctgggcc
     2761 tggccttgct gcggaccaac atggtgaagg aaggcgatgc ggccatcgcg aagtaccagc
     2821 ccgcgcttga ggccgccggt ggtgccgggc tgttgcccga tgaccagaag aaactgctca
     2881 agagcgcggt gatccactac gccgaatacc tcgacaccaa gggtaaccga tccaaggcca
     2941 aggaggtgct ggaggccgtg aaggagttct acgcggacga tgcggaattc aagggcgcac
     3001 tggaaagcta ctgaaacacc ggtgtgatgc cgggaatgga cggggcttca ggcgccgttc
     3061 gtgttccagg tgccatcaga gcctcctatg cccgcatacc ggggcgcctt ccgaacatgt
     3121 ggggcgatca gcgcttgtgc cgcagcaggt acaccaccag taccgggagc aacagc
//
LOCUS       DAOQOC010000213         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15494_length_3171_cov_5.029525, whole genome shotgun sequence.
ACCESSION   DAOQOC010000213 DAOQOC010000000
VERSION     DAOQOC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15494_length_3171_cov_5.029525"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>3171
                     /locus_tag="PL106_04585"
     CDS             153..>3171
                     /locus_tag="PL106_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32374.1"
                     /translation="MISGGADATYTTPAFVFNGPRFGLDIPSGGSWDLTYLHGMEQME
                     REFVITREPSSSVAPPGQQFIFQDDRASAGGYMYTATSLRFDIDGSSYSVPSVQPAPN
                     ATLDQFIISATDPNTLPIYNAILAAPPTGLPFTEVLNVSSLCFGPGDGTTLLNSTVRL
                     GWSCSYNASGTLCSSNPHTAKIRVGTRPSLVPEPAVFEGGYDDGCYYQGSVVRRIKYV
                     FHYPGNEGEIITVKGIRIGPFNNLESNTDLAINEDNVLLIRRVRPDGAVAEDSISTIS
                     GPGTSTRNQPAQGCGSPGTIDGFFAWSAPGNQLISKFPSLSPGDVLTISVPTRTCVQS
                     AALGGPCAIPYLPDHGKLELNLLYEYDTPCNAIDPAFLANGDVEHIAPENKCEVDYER
                     KANTTAIQAVPNDAGDLVTNMVTVCFDGVQPYQLAFTLNSAVTLLPTGSNERIALMLE
                     LDPGLYILPNELVGNFPLRVELADGTDVLSTELGTAPDALITVGQECEALDCPVGNDP
                     GWGQKQRLLLWLPSSVLPTSTDRIMTKLMQARIEFFVRMHCPMDADNDSCASSYVPGC
                     TSSPPAGSIPEIASAQLRLSLAHFLNGAAPCTGSDICSAPNVVIACHEQNYNLQCPGC
                     TRIGIHSQSFNMYRTSYGLVDQDDDGHADLVNGSVRRITTSEAKAGSGYGQGVLSTYM
                     MFGDSLEGDLETFIELDCGIYGPCVNDTPVVDLKYLYYKMTVTKPADTACSRLLKPIG
                     LDLIMYNDTDSTVVRIPESGLPLILSDTTNNVGETVYRYDLSPSVIASSLAQYIVMNE
                     IGWDPSFSFNAHDAAKIQVRPRFKVECNIGPMFQTAYVGNEIYLGARPETVASPLVDT
                     PGDFTEEGFCGSSPPGPCLDYQYWCTRKSGTFTVVGYVLRRKADYAKESPCANGPEII
                     YQLELGQNPTATVTGDNCFRNEYRNWFQLSSLSGGVELSEPSHWGAGTISVKEQVSGM
                     SMRTITLFSGQGTTNGNMSSYMTVDSIPTWPGTE"
BASE COUNT          695 a          932 c          871 g          673 t
ORIGIN      
        1 agaacgcgac attagtgtcg gtccccggtg gtttttcgct gacacagacc tcgggggcca
       61 ccatcaccgt gtccttcccg gtgcgtatga actgtgcgtt cgacttcaac gatgcgaatt
      121 cgttgagcga ccagtattgc tgttcgttca aggtgatcag cggtggtgcc gatgccacct
      181 acaccacacc ggcgttcgta ttcaatggac cgcgcttcgg tctcgacatt ccttccggag
      241 gttcatggga cctcacgtac ctccacggaa tggaacagat ggagcgggaa tttgtgatca
      301 cccgtgaacc cagttcatcg gtggccccgc caggtcaaca gttcatcttt caggacgacc
      361 gcgccagcgc gggcggctac atgtacacag ccacttcgtt gagattcgac atcgatggca
      421 gttcctattc tgtgcccagc gtacagccag ccccgaacgc aaccttggac caattcatca
      481 tcagtgccac agacccgaat accttgccga tctataacgc catcctggcc gcgccgccca
      541 ctgggcttcc gttcacggaa gtgctgaacg tcagttcgct ttgtttcggg ccgggtgatg
      601 gcacgaccct tttgaactcc acggtccgat tggggtggtc ctgcagttac aacgcatcgg
      661 gcacgttgtg cagctcgaac ccgcatacgg ccaagatacg ggtgggtacg agaccctcgc
      721 tggttcctga acctgcggtg ttcgagggcg gttacgatga tggttgctac tatcagggtt
      781 cggttgtgcg ccggatcaag tatgtcttcc actatccggg caacgagggt gagatcatca
      841 cggtgaaggg aataaggatc gggcccttca acaatctgga gtcgaacacg gacctggcca
      901 tcaacgagga taatgtgttg ctcattcgcc gtgtgcgtcc cgatggggca gtcgccgagg
      961 acagcatttc gaccatttct ggcccgggca ccagcacccg taaccagccc gcacaaggat
     1021 gcggatctcc tgggacgatt gacggcttct ttgcttggag cgcaccaggc aaccaattga
     1081 tctccaagtt cccgtccttg tcgcctggcg acgttctcac gatctctgtc cccactcgga
     1141 cgtgtgtgca gagtgccgcg cttggcggcc catgtgccat accatacttg ccggatcacg
     1201 gaaagctcga actcaacctg ctgtacgagt acgatacgcc ctgcaatgcg atcgatcctg
     1261 cattcctggc aaatggggac gtcgaacaca tcgctcccga gaacaaatgc gaggtcgact
     1321 atgagcgcaa ggccaatacc acagcgatac aggccgtgcc gaacgatgcc ggcgacctgg
     1381 tcaccaacat ggtaacggtc tgcttcgacg gggtacagcc ttaccagttg gcctttacat
     1441 tgaacagcgc agtgaccttg ttgccgaccg ggtccaacga acgcatagcc ctgatgcttg
     1501 aacttgaccc cggactttac atcctaccga acgaacttgt cggtaacttt cctctgcgcg
     1561 tcgaattagc ggacggcacc gacgtactaa gcacggaatt gggcacagcc ccggacgcgc
     1621 tcatcacggt cgggcaagaa tgcgaagcgc tggactgtcc agtgggcaac gatcccggat
     1681 ggggtcagaa gcagcgcctc ctgttgtggt tgcccagctc agtgctccct acttccaccg
     1741 atcgcatcat gaccaagctc atgcaggcgc gcatcgagtt tttcgtgcgc atgcattgtc
     1801 cgatggacgc ggataacgac agttgtgcgt cctcgtacgt gcccggatgc accagttcac
     1861 ctcctgcggg cagcattccc gagatcgcat ccgcgcaact tcgcctttcg ttggcgcact
     1921 tcctgaatgg tgcggcccct tgcacaggat cggacatctg ttcagccccc aacgtcgtca
     1981 tcgcatgcca cgaacagaac tacaacctgc agtgtccagg atgcacgcgc atcggcatac
     2041 acagtcaaag cttcaacatg tatcgcactt cctatggact ggtcgaccag gatgacgatg
     2101 gccatgcgga cctggtgaat ggatccgtaa ggcgaataac gacctctgag gcgaaggctg
     2161 gttcaggata cggccaaggc gtgttgagca cttatatgat gttcggtgac tcattggagg
     2221 gtgaccttga aaccttcatc gaacttgatt gtggaatata cggaccatgc gtcaacgaca
     2281 cgcccgtggt tgacctgaaa tacctgtatt acaagatgac cgtgaccaag ccggcagata
     2341 cggcctgtag ccgtctgcta aagcccatcg gtctggacct gatcatgtac aatgacaccg
     2401 acagcacggt ggtacgtata ccagaaagcg gcttgccgct gatcctgagc gataccacga
     2461 acaatgtcgg ggagacggta tatcgatatg acctcagccc atcagtgatc gcctcctctc
     2521 tcgcccaata cattgtcatg aacgaaatcg gctgggatcc atcgttctca ttcaatgcgc
     2581 atgatgcggc caagatccag gtccgccccc gattcaaggt cgagtgcaac atcgggccca
     2641 tgttccagac cgcctacgtg ggaaatgaga tctatctcgg agcaagacca gagaccgtcg
     2701 cgtcgccatt ggtggacact ccgggcgatt tcaccgagga agggttctgc ggtagttccc
     2761 cgcccggccc ctgtctggac taccaatact ggtgtacgcg gaaatcaggc acgttcacgg
     2821 tggtgggata tgtgctcagg cgcaaggccg actatgccaa ggaatcccct tgtgccaatg
     2881 gacctgaaat aatctaccag ttggaattgg ggcagaaccc gaccgctacc gtgaccggag
     2941 acaactgctt ccgcaatgaa taccggaact ggttccaact gtcttccctt tccggaggag
     3001 tagaactctc tgagccttcg cactggggcg ctggcaccat cagtgtgaaa gaacaagttt
     3061 ccggaatgtc catgcgtacc atcacgctgt tcagcggaca gggaacaacg aacggcaaca
     3121 tgtcgtccta catgacggtg gacagcattc cgacatggcc gggtacggag t
//
LOCUS       DAOQOC010000214         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15656_length_3154_cov_3.476605, whole genome shotgun sequence.
ACCESSION   DAOQOC010000214 DAOQOC010000000
VERSION     DAOQOC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_3154_cov_3.476605"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PL106_04590"
     CDS             complement(<1..967)
                     /locus_tag="PL106_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32375.1"
                     /translation="MKLDRTTYEAWLLDRIEGRLTPAQERELDAFLAANPDLSADTTG
                     LPTLESCTPKPIDWKDDLRKRLPPTGMPDAARLNEFLVARMEGDLNAEQEAALMKFLY
                     EHPEFEQDANRIRASRADNSPVPFTEKPSIERNFPPRGMPDKHRLTDFLIAAQEGDLD
                     RAQQQALDALLAHDPDAKKAQRIIAVSKVHAEHIVFPDKAGLKKREARVISLWQRYAV
                     AASIALLLGLAWWMLDADGGMGPERNGPSVAGTVSPSAQEPGKTNTEHGVHQPEEALP
                     IPDNGSNAGSTINPGGTRRQSEDGNTAKSNVNRAGGGAPDEHEGTP"
     gene            complement(964..1452)
                     /locus_tag="PL106_04595"
     CDS             complement(964..1452)
                     /locus_tag="PL106_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA32376.1"
                     /translation="MTLHDYNTCVDMHSDGVYRFALKHLRDRDEAKDVVQESFARLWQ
                     KVEEVDPSKAKSYLFTTAHHIIVDTARRGQRSLRMEEQHEDLSFTNEGSPPDLKRIID
                     AALATLPDIQRSVILLRDLEGYSYDEIAELTGLNLTQVKVYIYRGRTALKEYIGKLEV
                     VL"
     gene            1540..3060
                     /locus_tag="PL106_04600"
     CDS             1540..3060
                     /locus_tag="PL106_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32377.1"
                     /translation="MLDRIFRGLSSPWIMLVLMGGNTIALLCFRHFVSLDGPMHLLHA
                     VVLQEKWFGVPRSAAGITVDTSALDMNLGDLPLLLLSGRTHPFLLHKVLAAIAVGSVC
                     AGAWHLTRAYDRRPNAAWLLVLPFSVGFLMVLGVFHFILGAGIALGLCGWWVSRRAIR
                     WRQLALLLLGTALGTFAHRTGGLLALLLIGTQEIFAYWSDRGSWYGRWSALPARTPFA
                     LATIVFLIGVVLVAVGFATPVVHPHMEHHPVEELFTMRQILLVDREAEEPYRIALGVL
                     LVALLALSVRSRMRTRTPRTGDALLAMALVLFLLSMIRTARTELHYATDRAQWLGFVL
                     VACWIGIQDLPRWMMPMMLVAILYVHGLRLNLLEQWMHRLADEDAAALDIAASFTAGE
                     LVMPLVLDEDWLARHRTAYTAIGHHGILFTPRDHLRFRSLRPLDPEVRRYLASPDDDA
                     DNVLEHVRDGRFPVPERIVVIGGGGDEGKERLMTELLGERYVPSGGTGYARSWRRR"
BASE COUNT          490 a          928 c         1060 g          676 t
ORIGIN      
        1 caggagtgcc ctcgtgctca tccggggcac cacctcccgc tcggttcaca ttgcttttcg
       61 cagtgttccc gtcctccgac tgacggcgtg taccgccggg gttgatcgtg gagcctgcgt
      121 tgctgccgtt gtcagggatc gggagggcct cttcgggttg atgtacgcca tgttcggtat
      181 tggtcttgcc cggctcctgc gccgaagggg ataccgttcc ggcgaccgac ggcccgttgc
      241 gttctggtcc catgccaccg tccgcgtcca acatccacca agcgaggccc agcagcaagg
      301 cgatgctcgc ggccacggcg tagcgctgcc acagtgagat cacccgggct tcacgcttct
      361 tcagccccgc cttgtccggg aacacgatgt gttcggcatg gacttttgaa accgcgatga
      421 tgcgctgtgc cttcttggcg tcggggtcgt gcgccagtaa cgcatccagc gcctgttgct
      481 gggccctgtc caggtcgcct tcctgcgcgg cgatgaggaa atccgtcagg cggtgcttgt
      541 ccggcatgcc tcggggcggg aagttgcgct cgatggaggg cttctctgtg aacggtaccg
      601 ggctgttgtc agccctgctg gcgcggatac gattcgcatc ctgctcgaac tccgggtgct
      661 cgtacaggaa cttcatcaat gcggcctcct gttcagcgtt caggtcgcct tccatgcggg
      721 ccacgaggaa ctcattcagg cgggcggcgt cgggcatgcc ggtcgggggg agccgtttcc
      781 gaagatcatc cttccaatcg atgggcttgg gggtgcagct ctcgagggtc ggcagcccgg
      841 tggtgtccgc ggacagatcc gggttggccg ccagaaaggc atcaagttcc cgctcttggg
      901 cgggcgttag ccgtccctcg atacggtcaa gcagccaggc ctcgtatgtg gtgcgatcga
      961 gcttcatagg acgacctcca gtttcccgat gtattccttg agtgcggtgc ggccgcggta
     1021 gatgtacacc ttcacctggg tgaggttcag gccggtgagt tccgcgatct cgtcatagga
     1081 atatccttcc aggtcgcgca gcaggatcac cgagcgctgt atgtccggca gcgtggccaa
     1141 cgccgcgtcg atgatgcgct tgaggtcggg cggtgaccct tcgttggtga aggacaggtc
     1201 ttcgtgctgt tcctccatcc gcaggctgcg ttggccacgc cgtgcggtat ccacgatgat
     1261 gtggtgggcg gtggtgaaca ggtagctttt ggccttggag gggtccactt cctccacttt
     1321 ctgccagagc cgcgcgaaac tctcctgcac cacgtccttg gcctcatccc gatcccgcag
     1381 gtgcttcaag gcgaagcggt acacgccatc gctgtgcata tccacgcagg tgttgtagtc
     1441 gtgcagcgtc atcgggccct ggggagaggc acttcgggta taggacaggg aacggacggt
     1501 gaaagttaca ggagccactt gagtccgtaa ctcgccgcca tgctcgatcg gatcttccgg
     1561 gggttgagca gcccttggat catgctggtg ctcatgggcg gcaacacgat cgcgttgctc
     1621 tgcttccgcc atttcgtgtc cctggacggg ccgatgcatc tgttgcatgc ggtggtcctt
     1681 caggagaaat ggttcggcgt tccgcggtcg gccgccggta tcaccgtgga cacctcggcc
     1741 ttggacatga acctgggcga cctgcccttg ctactgctgt cggggcgaac gcatcccttc
     1801 ctgctgcaca aggtgttggc cgccatcgcg gtgggcagcg tgtgcgcagg tgcctggcac
     1861 ctgaccaggg cgtacgaccg acgccccaat gcagcatggc tgctcgtact gccgttcagc
     1921 gtaggtttcc tgatggtgct tggtgttttc catttcatcc tgggtgcggg catcgcactc
     1981 ggactgtgcg gttggtgggt gtcgcgaagg gcgatccgtt ggaggcagct ggctttgctt
     2041 ctgcttggca cggccttggg cacctttgca caccgcacgg gtgggctgct ggcactgctg
     2101 ctcatcggta cacaggagat tttcgcgtac tggagcgata gagggagctg gtatggacga
     2161 tggtccgcgt tgcccgctcg cacaccgttc gcgctggcga ccatcgtgtt cctgatcggg
     2221 gtcgtgctgg tggccgtggg cttcgcaacg cccgtagtgc atccgcacat ggagcaccac
     2281 cccgtggagg aattgttcac catgcgacaa atactactgg tggatcgtga ggccgaagag
     2341 ccgtacagga tcgcgctggg tgtgcttctc gtcgctttgc tggccctgtc ggtccgcagc
     2401 aggatgcgca cacggacgcc ccgaacaggc gatgcgctcc tggcgatggc actggtgctg
     2461 ttcctcctgt ccatgatccg tacggcgcgt accgagttgc actacgccac ggaccgggcg
     2521 caatggctgg gctttgtgtt ggtcgcatgc tggatcggca tccaggacct gccccgctgg
     2581 atgatgccga tgatgctcgt ggccattctg tatgtccatg gactccgact caacctgctc
     2641 gagcagtgga tgcaccgact ggccgatgag gatgcggctg cgttggacat cgctgcttca
     2701 ttcaccgccg gcgaactggt gatgcccctg gtgctggacg aggactggct ggcgcggcac
     2761 cgaacggcat acacggccat cggccaccac ggtatcctgt tcacaccgcg ggatcatctg
     2821 cgtttcaggt cactgcgccc actggatccg gaggtgcgcc ggtacctggc atcgccggat
     2881 gatgatgccg ataacgtgtt ggagcatgtg cgcgatggtc gcttcccggt tccggagcgt
     2941 atcgtggtga tcggcggtgg aggggatgag gggaaggaac ggttaatgac cgaactactt
     3001 ggagaaaggt acgtaccatc gggcggaacg ggctacgcac gctcatggcg gcggcggtga
     3061 aggaggcagg tccgggatcg gaagatggcg gaaggggcag gagcgcgccg aaggaccatc
     3121 cgcgtagtga tggtcgagga ggaccgaccg cagt
//
LOCUS       DAOQOC010000215         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15666_length_3153_cov_3.863783, whole genome shotgun sequence.
ACCESSION   DAOQOC010000215 DAOQOC010000000
VERSION     DAOQOC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15666_length_3153_cov_3.863783"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="PL106_04605"
     CDS             complement(<1..1429)
                     /locus_tag="PL106_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32378.1"
                     /translation="MARLDSFRSACLGSALLLLAGCSNEKDAFLNRTFHRLTARDNGW
                     FNANEKLKETVAAMEKAYVDDYDEVLPIFIIGSEDQAKAMVPDLEICIDKCSTVIERH
                     SMDIGKGEKNTWIDDAYFVAGRSHYYKRAYFDAERTFDYAARRFKGMNRQMECKLWFA
                     RTAIQNEQYAKAQSALDEIKNQKELPKKFPHDQLSATQADVYLRRGKVDDAIVDLEHA
                     VSICRNRGQRSRWMFILAQLYMAKGMEDKAIATFNKVARSNAPYELGFHAEIFQALSS
                     MQGNTEQARKKLRRMRRDEKNKDHFDMIEYALADLDLKENKDSSAVAHLKESARVSTT
                     DTKQKAKTFLRLADYFFDDKRYPPAQQYYDSTRTLLAETHKRFEEVNTRADVLGELVE
                     QLNIIAFEDSVQAVAKMDPEEREKLIKQRIKERQREEDEKEAAEAAAREAQTDPNAGQ
                     NKPPQGGGDASAWYFYNPTQIARGLA"
     gene            1530..1967
                     /gene="dut"
                     /locus_tag="PL106_04610"
     CDS             1530..1967
                     /gene="dut"
                     /locus_tag="PL106_04610"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNA32379.1"
                     /translation="MTNVRIINRGHHPLPQYATPHSAGLDLRANLDRTVILAPGQRAL
                     IPTGLFLELPPGTEAQVRPRSGLALKYGITVLNAPGTIDADYRGELGVLLINHGQESF
                     AVQDGERIAQLVVARYEQVSFAEVTDLAVSERGGGGFGHTGRK"
     gene            2115..3122
                     /locus_tag="PL106_04615"
     CDS             2115..3122
                     /locus_tag="PL106_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA32380.1"
                     /translation="MKIIVPMAGMGKRMRPHTHTTAKPLLPIAGKPIVQRLVEDLAAV
                     AGEPVEEVAYIVHPSFGKKVEDELVGIAKAIGSKGTIHYQETALGTAHAILCAEKALS
                     GRIIVAFADTLFRARLKLDKDCDGVIWVNKVEDPRPFGVVKLDNLGIITEFVEKPQQF
                     VSDLAIIGIYYFADGEYLRKEMKYLIDNDIKDKGEYQLTNAMENMKRKGARFKAGSVD
                     VWMDCGNKNAMVDTNSRVLGFLNGSKDLVSATLKLENSVMVPPCFIGENVTLVNSVVG
                     PNVSIEQGTVVTNSVVRDSILRGDSRVQDAVVANSMIGQRAMVKGRVLDLSLSDDATI
                     G"
BASE COUNT          590 a          972 c          956 g          635 t
ORIGIN      
        1 cggccaggcc gcgtgcgatc tgtgtcgggt tgtagaaata ccacgcactg gcatcaccac
       61 caccttgggg gggcttgttc tgcccggcat tggggtcggt ctgcgcttcg cgcgcggcgg
      121 cctcggcggc ctccttctcg tcctcctccc gctggcgctc cttgatgcgc tgcttgatca
      181 gcttctcgcg ctcctcggga tccatcttcg ccacggcctg cacgctgtcc tcgaaggcga
      241 tgatgttgag ctgctccacc agttcaccca acacgtcggc gcgggtgttc acctcttcaa
      301 aacgcttgtg ggtctcggcc agcagggtgc gggtgctgtc gtagtactgc tgggcgggcg
      361 ggtagcgttt gtcgtcgaag aaatagtccg ccaggcgcag gaaggtcttg gccttctgct
      421 tggtgtcggt ggtgctcacc cgcgcgctct ccttcagatg ggccacggcg ctgctgtcct
      481 tgttctcctt caggtcaagg tcggccaggg cgtactcgat catgtcgaag tgatccttgt
      541 tcttctcgtc ccggcgcatg cggcgcagct ttttgcgcgc ctgttcggta ttgccctgca
      601 tgctggagag cgcctggaag atctcggcgt ggaaacccag ttcgtacggt gcgttgctgc
      661 gtgccacttt gttgaaggtg gcgatggcct tgtcctccat ccctttggcc atgtacaact
      721 gggccaggat gaacatccag cgcgaacgtt gtccgcggtt ccggcagatg ctcacggcgt
      781 gttcaagatc cacgatggcg tcgtccactt taccgcgccg caggtataca tcggcctggg
      841 tggcgctcag ctggtcgtgg gggaatttct tgggcagctc cttctggttc ttgatctcat
      901 ccagcgcact ctgtgctttg gcgtactgct cgttctggat ggccgtgcgc gcgaaccaca
      961 gcttgcactc catctgccgg ttcatgcctt tgaagcggcg cgcggcatag tcgaaggtcc
     1021 gctccgcatc aaaatacgcg cgcttgtaat agtggcttcg gcccgccacg aaataggcat
     1081 catctatcca ggtgttcttt tcccctttgc cgatatccat ggagtgccgt tcgatcaccg
     1141 tgctgcactt gtcgatgcag atctccaggt cgggcaccat ggccttggcc tggtcctcgc
     1201 tgccgatgat gaagatgggc agcacctcgt cgtaatcgtc cacataggcc ttctccatgg
     1261 ccgcaacggt ctccttcagc ttctcgttgg cgttgaacca gccattgtca cgtgcggtga
     1321 ggcggtggaa cgtgcggttc aaaaaggcgt ccttctcgtt tgagcaccct gcgagcaaca
     1381 gcaaggccga tcccaagcag gcggaacgga aggagtcgag gcgggccacg ggatgagggg
     1441 cgtgcggcta taacagaaag gcggccaagt tatttcatcg ggcgcagagc cgacctttgc
     1501 accgccgcct gacattcggc acctgctaca tgaccaacgt acggatcatc aaccggggcc
     1561 accacccact accacagtac gccaccccgc acagcgcggg cctcgatctg cgggccaacc
     1621 tggaccgcac ggtgatcctg gcgcccggcc aacgggcatt gatccccacg ggcctgttcc
     1681 tggagctgcc cccgggcacc gaagcacagg tgcggccccg tagtggactt gcgctcaaat
     1741 acggtatcac cgtgctcaac gcaccaggca ccatcgacgc cgactaccgg ggcgaattgg
     1801 gcgtactgct gatcaaccac gggcaggaat ccttcgcggt tcaggatggt gagcgcattg
     1861 cgcagttggt ggtcgcccgc tacgaacagg tgagcttcgc cgaggtaact gaccttgcgg
     1921 tgtcagaacg cgggggtggc ggcttcggac atactgggcg gaaataggca ccttcgcggc
     1981 caattcttca ccatagcggt acaactggaa actgcgcagt ccctaccacg cccgatgacc
     2041 gggcttccgc gcgtcagttc catcgaatca tccccacaca tccggcgctc ccttttggtg
     2101 aaccctgaac agaaatgaag atcatcgtcc ccatggcggg catgggcaag cggatgcgtc
     2161 cgcacaccca caccaccgcc aagcccctgt tgccgatcgc cggcaagccc attgtacaac
     2221 gcctggtgga agatctggcc gcggtggccg gagaacccgt ggaagaggtc gcctacatcg
     2281 ttcaccccag cttcggaaag aaggtggagg acgaattggt cggcatcgcg aaggccatcg
     2341 gcagcaaagg caccatccac tatcaagaga ccgctctcgg tacggcccat gccatcctct
     2401 gcgccgaaaa agccctctct ggccgcatca tcgtggcctt cgccgatacg ctgttccggg
     2461 cccggctcaa actcgacaag gattgcgacg gtgtcatttg ggtgaacaaa gtggaggatc
     2521 cgcgaccctt tggcgtggtg aaactggaca acctcgggat cattaccgag ttcgtggaaa
     2581 agccgcagca attcgtgagc gacctggcca tcatcggcat ctactacttc gcggacgggg
     2641 agtacctccg caaggagatg aagtacctga tcgacaatga catcaaggac aagggcgagt
     2701 accagctgac caacgccatg gagaacatga agcggaaggg tgcacgcttc aaggccggca
     2761 gtgtggacgt gtggatggac tgcggcaaca agaacgccat ggtggacacc aacagccgtg
     2821 tactcggctt cctcaacggg agcaaggacc tggtaagcgc cacgttgaag ttggagaaca
     2881 gcgtgatggt cccgccctgc ttcatcgggg agaacgtgac ccttgtgaac agcgtggtag
     2941 gaccgaacgt gagcatcgag cagggcacgg tggtcaccaa cagcgtggtg cgcgattcga
     3001 tcctccgcgg tgattcgcgt gtacaggatg cggtggtcgc caacagcatg atcgggcaac
     3061 gcgccatggt gaagggccgg gttctggacc tcagcctcag cgacgacgcc acgatcggat
     3121 gatctttgta cccgatgcgc cccaccccca ccc
//
LOCUS       DAOQOC010000216         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15744_length_3146_cov_3.525073, whole genome shotgun sequence.
ACCESSION   DAOQOC010000216 DAOQOC010000000
VERSION     DAOQOC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15744_length_3146_cov_3.525073"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PL106_04620"
     CDS             <1..536
                     /locus_tag="PL106_04620"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNA32381.1"
                     /translation="MDVDGFLCYMAAQIILANTDWPEQNLKWWRWTGTPDTLGGPGDG
                     RWRFIMTDSDLGMGLTVPPNYDMFAHIDRHAAVPVVRLLKACLRSTLLKDRFARILDE
                     LLAGPLSADRMTAEVERMRDAISLEMPVHIGRWRRPGSVEEWNDHVEELLTFAAQRPA
                     AVKARSAIWLARYPLRP"
     gene            533..1915
                     /locus_tag="PL106_04625"
     CDS             533..1915
                     /locus_tag="PL106_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32382.1"
                     /translation="MTAFIKEHSRIYVLIMIWAATIWFAGPLFYLVLPASVFLMRSRD
                     MWPDMLFGFLVILIWSDMNPGIVPFRTVKTAKNTYIVALAIIYFMETARMQPSAGIFK
                     VFLPFFGFSFLPIVASGEPLTSIQKTVSYALLYLVIPNHVLFCYRRQGWVFFRHLVIF
                     IVVFLALQRALPYFTPHWWSFVAGRFRGFFGNPNGLSIFCYLCFMLVTVLFSVKPDLF
                     SFKGKVVVYAVIVYFLIEGGSRTSVTSTLMFMLFYRFFAASPFLGFVAFLAFGAVVEA
                     VSSNLPAILNALGLHEYFRVETIADGSGRYFAWSFAWSKINEGGFFLFGGGFGNDEFI
                     MRQHYPYLRTMGHHGGVHNSYLTMWFNVGIVGILIFFRSFLLIFAKAVKSAPYAFAIM
                     FSVMFSVLYESWITGSLNPFTIILLCILTVLSEDEIVNWRAHVATNDAGGVEEAPTVE
                     PVPRLILPAR"
     gene            1943..>3146
                     /locus_tag="PL106_04630"
     CDS             1943..>3146
                     /locus_tag="PL106_04630"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNA32383.1"
                     /translation="MKKRWPWIAALLLVILSGSAWFADRKLKKHGHPGLRTFVAQWWN
                     NYPKSFAIEPPVVSITVDDAGMEQLYGVVERARDLGVIQREGNEYVDGEFSVAMPGGG
                     EGSGDFKGKLRIKGKMTDHVEGEKWSFRVVARKSGGFLGMKRFSLQHPGTRNYLCDWF
                     FHRLMKGEGIIAPRYGFCKVKLNDEDLGVYAYEEHFGPELLENNDRVPGPIVRFDPGL
                     FWLHRTQGIDGSAKVDDAYGAYQAAGLDAFGTDDIAKDPDQKRYFEQAIALMEGFRRG
                     RLRASQVFDVERTGKRLAMLDLVGGHHSMDWSDVKFWFDPMAQRLEPISYESFSAERI
                     NQLAGAWRVTGPFSPSDELHTALFKDPAIFSAYVRNMERFSGQEWLDSAFAALAGALD
                     AASATLYGE"
BASE COUNT          553 a          969 c          940 g          684 t
ORIGIN      
        1 ggatggacgt ggacggattc ctttgctaca tggcggcgca gatcattctg gcgaataccg
       61 actggccgga acaaaacctc aaatggtggc gctggaccgg cacaccggac accttgggcg
      121 gtcccggcga tggccgctgg cgcttcatca tgaccgacag cgatctgggc atgggcctca
      181 ccgtgccgcc caactacgac atgttcgccc acatcgatcg gcacgcggcc gttcccgtgg
      241 tccgattact caaggcctgc ctccgttcca cgctcctgaa ggaccgtttc gcgcgcatcc
      301 tggacgaatt gctggccgga ccgctatccg ccgatcggat gaccgcggag gtggagcgca
      361 tgcgcgatgc catctccctt gaaatgcccg tacatatcgg tcgttggcgc cgtccgggat
      421 cggtggaaga gtggaacgac cacgtggagg aactcctcac cttcgcggcc caacgcccgg
      481 ccgccgtgaa agccaggtcc gccatctggc tcgcacggta cccgctacgc ccatgacggc
      541 cttcatcaaa gagcacagcc ggatctacgt gctgatcatg atctgggcgg ccaccatctg
      601 gttcgccggg ccgctgttct atctggtctt gccggcaagc gtcttcctta tgcgtagccg
      661 ggacatgtgg cccgacatgc tcttcggctt cctggtcatc ctcatctggt cggatatgaa
      721 cccggggatc gtgcccttcc gaacggtgaa gaccgccaag aacacataca tcgtggcact
      781 ggcgatcatc tacttcatgg agaccgcccg catgcaaccc tcggccggga tcttcaaggt
      841 gttcctgccc ttcttcggct tctccttcct gcccatcgtg gcctcgggtg agccgctcac
      901 ctccatccag aagaccgttt cctacgcgtt gctctacctg gtgatcccga accacgtact
      961 gttttgttac cggagacaag ggtgggtctt ttttcgccac ctggtcattt tcatcgtcgt
     1021 attcctcgcc ttgcaacggg ccctgccata tttcacacca cactggtgga gtttcgttgc
     1081 cggtcgtttc cggggcttct tcggcaaccc gaacgggctg tccatcttct gctatctgtg
     1141 cttcatgctc gtcacggtcc tcttctccgt gaaaccggat ctcttcagct tcaaaggcaa
     1201 ggtggtcgtg tacgccgtga tcgtgtactt cctgatcgag ggaggttcgc gcacctcggt
     1261 cacgtccacg ttgatgttca tgctgttcta ccgcttcttc gccgcatccc cgttcctggg
     1321 cttcgtggcc ttcctggcct tcggcgcagt ggtggaggcc gtctcgagca acctgccagc
     1381 gatactcaac gcgctgggct tgcacgagta cttccgggtg gagaccatcg ccgatgggtc
     1441 cggtcgctat ttcgcctggt ccttcgcgtg gagcaagatc aacgagggcg gtttcttcct
     1501 gttcggcggc ggtttcggca acgacgagtt catcatgcgc cagcactatc cctatctgcg
     1561 cacgatgggc caccatggcg gtgtacacaa cagctacctc accatgtggt tcaatgtcgg
     1621 catcgtgggg atcctgatct tcttccggag cttcctgctc attttcgcca aggccgtgaa
     1681 gagcgcaccg tacgcgttcg ccatcatgtt ctccgtcatg ttcagcgtgc tctacgagag
     1741 ttggatcacc ggatcgctga accccttcac catcatcctg ctctgcatcc tcaccgttct
     1801 ttccgaggac gagatcgtga attggcgggc ccatgtggcc acgaatgatg cggggggcgt
     1861 ggaggaagcg ccgaccgtgg aacccgtgcc caggttgatc cttcctgcgc gctgagccgg
     1921 gctacttttg tcgccctttc ccatgaagaa acgttggccc tggatcgctg ccctgctcct
     1981 cgtgatcctg tccggttccg cctggttcgc cgatcgcaag ctcaagaagc atggccatcc
     2041 gggcctccgc accttcgtcg cccaatggtg gaacaactac ccgaagagct tcgccattga
     2101 acccccggtg gtctcgatca ccgtggatga tgccggtatg gagcaactgt acggcgtggt
     2161 ggaacgtgcc cgtgatctcg gtgtgatcca acgcgagggc aacgagtacg tggatggtga
     2221 gttcagcgtg gcgatgcccg gtggagggga gggttccggt gatttcaagg gcaagctgcg
     2281 catcaaagga aagatgacag accacgtgga aggtgaaaag tggagtttcc gcgtcgtggc
     2341 ccgaaaaagt ggtggatttc tcggtatgaa gcgtttttcg ctgcagcacc ccggcacgcg
     2401 caactacctc tgtgactggt tcttccatcg gctcatgaag ggcgaaggga tcatcgcgcc
     2461 ccgttacggg ttctgcaagg tgaagctcaa cgacgaggac ctgggcgtgt acgcgtacga
     2521 ggagcatttc ggcccggagc tgctggagaa caacgatcgt gtaccgggtc ccatcgttcg
     2581 cttcgatccc ggtttgttct ggctccaccg cacacagggc attgatgggt cggccaaggt
     2641 ggacgatgcg tacggcgcct accaggcggc cggtctcgat gcgttcggga cggacgatat
     2701 cgcgaaggat ccggatcaga aaaggtactt tgaacaggcg atcgcactca tggaagggtt
     2761 ccggcgaggc agactgcgtg cgagccaggt attcgatgtg gagcgcaccg gaaagcgcct
     2821 ggccatgctc gatctggtgg gcgggcacca cagcatggac tggagcgatg tgaagttctg
     2881 gttcgatccg atggcgcaac ggctggaacc catcagctac gagagcttca gcgccgagcg
     2941 catcaaccaa ctcgccggtg cctggcgcgt gaccgggccg ttcagtccga gcgacgaact
     3001 gcacaccgcg ctcttcaagg atccggcgat cttctccgca tacgtgcgca acatggaacg
     3061 gttcagcggg caggaatggc tggacagtgc cttcgccgcg ctggccggtg ccctggatgc
     3121 cgccagtgcc accctctacg gcgagt
//
LOCUS       DAOQOC010000217         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15745_length_3146_cov_3.439340, whole genome shotgun sequence.
ACCESSION   DAOQOC010000217 DAOQOC010000000
VERSION     DAOQOC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15745_length_3146_cov_3.439340"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..2498
                     /locus_tag="PL106_04635"
     CDS             51..2498
                     /locus_tag="PL106_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA32384.1"
                     /translation="MDYLKGLNPAQLTAVQHTDGPNMVIAGAGSGKTRVLTVRIAHLM
                     AAKGVDPFRILALTFTNKAAREMKERIEKLLRTVPDLRTEARNLWMGTFHSVFARILR
                     AEAEKLGYPRDFTIYDTDDSRSLIKTIVKERQLDDKMYKASNVHARISISKNNLLGPE
                     EYLRDAEVMAADAAALRPKMGELYKAYAARCFRAGAMDFDDLLYNTNILFRDHPETMV
                     KYQSRFQYILVDEYQDTNLAQYNIVRTLAARHENITVVGDDSQSIYGFRGANIQNILN
                     FRRDYPDQRLMKLEQNYRSTKTLVAAANSLIDKNRDRIQKEIWTDNTEGEPIRVQRTV
                     SDNEEGFFVAHDIFETRMREQAANKAFAILYRTNAQSRSMEEALRKLNIPYRIHGGLS
                     FYQRKEIKDLIAYFRLTCNPNDEEALKRVINYPARGIGQSTVDKLTVAAGERQKSIWD
                     TITGHLGELGLHEGLRKRLADFVLMIRSFQTMLDTQSAHALGEYIARTTGLLQDLFND
                     KTPEGVSRYENIQELLNGMREFAEVPAGEGEAPEPRPVRTLSDFLIDVALLTDADKDD
                     PNDNDRVSLMTIHSAKGLEFPYVYIVGLEEELFPNAMSVHSRTDLEEERRLFYVALTR
                     AEKRATLSYAMSRYKWGNLTAGEPSRFIDEIDPKYLVLPRRHEAPSFNRDSLASSWVR
                     PGNTSTRPVAGGTVFGREPKKAAPAPRTTPSPVQRGNAPSPGSGAAPSIDPEKLKKLK
                     RVSNTGAPLEATRTLGHAAPADIAEGATVEHERFGRGKVLKVEGKEPDLKATIFFPST
                     GQKNLLLRFAKLRVVEG"
     gene            2538..>3146
                     /locus_tag="PL106_04640"
     CDS             2538..>3146
                     /locus_tag="PL106_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA32385.1"
                     /translation="MFSAFDPAYECGYAAGAAFVLAACVMHLKGHPRTAIALLALGAT
                     VLRCVAAALDPFLNDWDEAYHAVVAKNLMEHPLTPMLYRETALPISSHWPEQHIWLHK
                     PPFFLWQIALSLKLFGLHPWAVRLPSVFWTSLLVPVIWRMGTLLRNERTGFIAAALCA
                     CSYLLQELTAGMINTDHNDSVFIATVACSWLAWLEHVRNASLR"
BASE COUNT          651 a         1076 c          901 g          518 t
ORIGIN      
        1 taacccttcc cggccgcccc ttgcccggcc tgtaccttcg gccgctcaca atggactacc
       61 tgaaaggcct caatcccgcg caactcacgg cggtgcagca taccgacggc cccaacatgg
      121 tgatcgccgg ggccggctcc ggcaaaaccc gcgtgctcac cgtgcgcatc gcccacctga
      181 tggccgccaa gggcgtggac cccttccgca tcctcgccct cacttttacc aacaaggccg
      241 cgcgcgagat gaaggagcgc atcgaaaagc tcctgcgcac cgtgcccgac ctccgtaccg
      301 aggcgcgcaa cctctggatg ggcaccttcc acagtgtgtt cgcccgcatc ctccgcgccg
      361 aggccgaaaa actcggctac cccagggact tcaccattta tgataccgac gacagccgat
      421 cgctcatcaa gaccatcgtg aaggagcggc aactggatga caagatgtac aaggccagca
      481 atgtgcatgc ccgcatcagc atcagcaaga acaacctgct gggacccgag gagtacctgc
      541 gcgatgccga ggtgatggcc gctgacgccg cagccctgcg cccgaagatg ggcgagctgt
      601 acaaggcata cgccgcccgc tgtttccggg cgggggccat ggatttcgac gacctgctct
      661 acaacaccaa catcctcttc cgcgaccatc cggagacgat ggtgaagtac cagtcgcgtt
      721 tccaatacat cttggtggac gagtatcagg ataccaacct ggcccagtac aacatcgtgc
      781 gcaccctggc cgcgcgccac gagaacatca ccgtggtggg cgacgacagc cagagcatct
      841 acggcttccg cggcgccaac atccagaaca tcctcaactt ccggcgcgac taccccgacc
      901 agcggctgat gaagctggaa cagaactacc gcagcacgaa aacactggtg gccgcggcca
      961 acagcctcat cgacaagaac cgcgaccgca tccagaagga gatctggacc gacaacaccg
     1021 agggcgagcc catccgcgta caacgcaccg tgagcgacaa cgaggaaggt ttcttcgtgg
     1081 cgcacgacat tttcgagacc cgcatgcgcg aacaggccgc caacaaggcc ttcgccatcc
     1141 tttaccgcac caacgcccag agccgcagca tggaagaggc cctgcgcaag ctgaacatcc
     1201 cctaccgcat ccatggcggc ctcagcttct accagcgcaa ggagatcaaa gacctcatcg
     1261 cctacttccg cctcacctgc aaccccaacg acgaggaggc gctcaagcgt gtgatcaact
     1321 accccgcacg cggtataggg cagtccacgg tggacaaact gaccgtggcc gccggggagc
     1381 gccagaagag catctgggac accatcacgg gacacctcgg cgaactgggc ctgcatgaag
     1441 ggctgcgcaa acgcctggcc gacttcgtgc tgatgatccg cagcttccag accatgctgg
     1501 acacgcagag cgcacacgcc ctgggcgagt acatcgcccg caccaccggg ttgctgcagg
     1561 acctcttcaa cgacaagacg cccgagggcg tgagccgcta cgagaacatc caggaactgc
     1621 tcaacggcat gcgcgaattc gccgaagtgc ccgccgggga aggggaggcc ccggagccgc
     1681 ggcccgttcg cacactcagt gatttcctca ttgatgtggc cctgctcacc gatgccgaca
     1741 aggacgatcc gaacgacaac gaccgcgtga gcctgatgac catccacagc gcgaagggac
     1801 tggagttccc ctacgtgtac atcgtgggcc tggaggagga attgttcccg aacgccatga
     1861 gcgttcacag ccgtaccgat ctggaggagg agcgccgcct gttctatgtg gcgctcacac
     1921 gggctgagaa acgcgccacg ctcagctacg ccatgagccg gtacaaatgg ggcaatctca
     1981 ccgcggggga acccagccgc ttcatcgatg agatcgatcc caagtacctg gtacttccac
     2041 gccgccacga ggccccgtcc ttcaaccgcg acagcctcgc atcgtcgtgg gtgcgcccgg
     2101 gcaacacctc cacacgcccc gtggccggcg gcacggtgtt cggacgcgag ccgaagaagg
     2161 ccgcacccgc accgcgcacc acgccttcgc cggtgcaacg cgggaatgca ccttcgcctg
     2221 gatcaggagc cgccccgtcc atcgatcctg aaaagctgaa aaaactgaaa cgcgtgagca
     2281 acaccggggc gccattggag gccacccgca ccctggggca cgcggccccg gccgacatcg
     2341 ccgagggtgc taccgtggaa catgaacgtt tcggtcgtgg caaggtgctc aaggtggaag
     2401 gtaaggaacc tgacctcaag gctaccatct tcttccccag taccgggcag aagaacctct
     2461 tgttgcgctt cgcgaagctg cgggtggtgg agggctgacg agcgtgtccc ggacattgct
     2521 tgttttgtcg gaccatcatg ttctccgcgt tcgatccagc ctatgaatgc ggttacgcag
     2581 cgggtgctgc ctttgtgctc gcggcgtgtg tgatgcacct gaaggggcac ccgcgtacgg
     2641 ccatcgcact gcttgctctg ggtgccaccg tgctccgctg cgttgctgcc gcgctggatc
     2701 ccttcctgaa cgattgggat gaagcctacc acgccgtggt ggcgaagaac ctgatggagc
     2761 atccgctcac accgatgctc tacagggaaa cggccctgcc gatctcctcg cattggcccg
     2821 aacagcacat ctggctgcac aaaccgccct tcttcctctg gcagatcgcg ctttcgctca
     2881 agctcttcgg gctgcatccg tgggccgttc gcctgccgag cgtgttctgg acaagtctcc
     2941 tcgtacccgt gatctggcgc atgggcacgc tgctgcgcaa cgaacgcacc gggttcatcg
     3001 ctgctgcgct ttgcgcgtgt tcctacctgt tgcaggaact caccgcgggc atgatcaata
     3061 ccgatcacaa cgattcggta ttcatcgcca ccgtggcctg ctcctggttg gcctggctcg
     3121 agcacgtacg caacgcgtcg cttcgg
//
LOCUS       DAOQOC010000218         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15791_length_3142_cov_3.122449, whole genome shotgun sequence.
ACCESSION   DAOQOC010000218 DAOQOC010000000
VERSION     DAOQOC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15791_length_3142_cov_3.122449"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PL106_04645"
     CDS             <1..630
                     /locus_tag="PL106_04645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809104.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNA32386.1"
                     /translation="DNLLVRNRIFMDRTINCGAFPADRALAYGFTGPNLRACGVDYDV
                     RVADPYSGYQDFDFMVPIGQSGDTYDRFMVRDQEMKESISIIKQALDKLDKLSDKKSY
                     TADVPEWVLPPKENVYNQMEALIWHFKIVMGETNVPMGEVYHCVEGANGELGFYLVSD
                     GGRTPARLHFRRPCFIYYQAYPEMIKGTMLSDAILTMSSMNVIAGELDA"
     gene            632..1186
                     /gene="nuoE"
                     /locus_tag="PL106_04650"
     CDS             632..1186
                     /gene="nuoE"
                     /locus_tag="PL106_04650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNA32387.1"
                     /translation="MGATLKAVTTTEGTPPFSFSAEGLAECQRIIARYPEGRSKSALL
                     PILHIAQAENDGWLSVNAMDAVAQVLGIQHIEVYEVASFYSMYNLHPVGKHLLEVCHT
                     SPCMIRGSDDIVAYLEKKLNIHVGETTKDGMFTLKTVECLGSCGTAPMLQCGAKYHEN
                     LTPDKVDALIESLRNQPRNNYTDR"
     gene            1282..2634
                     /gene="nuoF"
                     /locus_tag="PL106_04655"
     CDS             1282..2634
                     /gene="nuoF"
                     /locus_tag="PL106_04655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNA32388.1"
                     /translation="MGRKLLLEHVNKPGIQGLEGYRANGGYRSVEKALKTMGPEAVLE
                     EVKKSGLRGRGGAGFPTGLKWSFMAKPPGIPRYLVVNADESEPGTFKDRYLMERIPHM
                     LVEGMITSSFALGANTSFIYIRGEYFYVSRILERAIEEARAAGWLGKNILGSGFDHDI
                     HVHVGAGAYICGEETALLESLEGKRGNPRIKPPFPAVKGLYECPTVVNNVEAIAAVVP
                     IVNDGGEEYAKIGIGKSTGTKLISAGGNIKRPGVYEIELGVSCEEFLNSDEWCGGVEG
                     RALKAVIPGGSSVPIIPAELYFKTAKFETRLMTYESLSDGGFATGTMLGSGGFYAYND
                     AQCIVRSTWNHSRFYRHESCGQCSPCREGTGWMEKILYRLEHGQGRTADIDLLWDIQR
                     RIEGNTICPLGDAAAWPVASAIRHFRDEFEYHATHPTKVKDPKHFEKEPFRRVAKAVV
                     "
     gene            complement(2648..>3142)
                     /locus_tag="PL106_04660"
     CDS             complement(2648..>3142)
                     /locus_tag="PL106_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNA32389.1"
                     /translation="PHTTRAWLDTLEQTFMVRTLQPWYENLGKRLVKSPKIYLRDSGL
                     FHALQGVKDTAALFAHPKLGASWEGFALEETLAAFRPHQVWFYAVHSGSELDLFFLHN
                     GMRTGVEFKREDAPRATRSMRTALADLQLDRLYVVYPGTRRYELDDRIACVPLEEVHG
                     LAAA"
BASE COUNT          658 a          947 c         1014 g          523 t
ORIGIN      
        1 gacaacctgc tcgtccgtaa caggatcttc atggaccgga ccatcaactg cggggccttc
       61 ccggccgatc gtgccctggc ctacggcttc accggcccca acctgcgcgc ttgcggtgtg
      121 gactacgacg tgcgcgtggc cgatccgtac agcgggtatc aggacttcga cttcatggtg
      181 cccatcggac agagcgggga tacctacgac cgcttcatgg tgcgtgatca ggagatgaag
      241 gagagcatca gcatcatcaa gcaggcgctg gacaagctgg acaagctgag cgataagaag
      301 tcgtacaccg ccgacgtgcc cgaatgggtg ctgccgccca aggagaacgt gtacaaccag
      361 atggaagccc tgatctggca cttcaagatc gtcatgggcg agacgaacgt gccgatgggc
      421 gaggtgtacc actgcgtgga aggagcgaac ggcgaactgg gcttctacct cgtgagcgat
      481 ggtggccgta ccccggcgcg gctgcacttc cgccgaccct gcttcattta ttaccaggcc
      541 tacccggaga tgatcaaagg caccatgctg agcgacgcga tcctcaccat gagcagcatg
      601 aatgtgatcg cgggtgaact cgacgcgtga gatgggagca actctgaaag ccgtcaccac
      661 caccgaaggc acgccgccct tcagcttcag tgcggaaggc ctggccgagt gccaacgcat
      721 catcgcccgt taccccgaag gacggagcaa aagcgcgctg ttgcccatac tccacatcgc
      781 ccaagcggag aacgatggct ggctgagcgt gaacgccatg gacgccgtgg cccaggtgct
      841 cgggatccag cacatcgaag tgtacgaagt ggccagcttc tacagcatgt acaacctgca
      901 cccggttggc aagcacctgc tggaggtgtg ccacacgagc ccgtgcatga tccggggcag
      961 cgacgacatc gtggcctatc tggagaagaa gttgaacatc cacgtgggcg agaccaccaa
     1021 ggacggcatg tttaccctga agaccgttga atgcctcggt agctgcggta cagctcccat
     1081 gctgcaatgc ggtgcgaagt accacgagaa cctcaccccg gacaaggtgg atgcgctgat
     1141 cgaaagcctg cggaaccagc cgaggaacaa ttacactgac cgatgaaacc tgagtggcga
     1201 gtgacgagtg acgagagacg agtggcacat gccacgggct ttctcaccac tcgtcactcg
     1261 tcactcgtct cttcctgagc aatgggacgc aaactcctcc ttgagcacgt caacaaaccc
     1321 ggcatccagg ggctggaggg ctaccgcgcg aatggtggct accgcagcgt ggagaaggcg
     1381 ctgaagacca tgggtccgga agcggtgctg gaagaggtga agaagagcgg cctgcgtggt
     1441 cgtggtggtg ccggtttccc cacgggcttg aagtggagct tcatggccaa gcctcccggt
     1501 attccgcggt acctggtggt gaatgccgac gagagcgagc cgggcacctt caaggaccgc
     1561 tacctcatgg agcggatccc gcacatgctg gtggagggca tgatcacgtc cagcttcgcc
     1621 ctgggtgcga acacgagctt catctacatc cgcggagagt atttttatgt gagccgcatc
     1681 ctggagcggg cgatcgaaga ggcccgcgcg gccggctggc tgggcaagaa catcctgggc
     1741 agcggtttcg accacgacat ccatgtccat gttggtgcgg gcgcgtacat ctgcggcgaa
     1801 gaaacggccc tgctcgaaag cctggagggc aagcgcggca acccgcgcat caaaccgccc
     1861 ttcccggcgg tgaaaggcct gtacgagtgc cccaccgtgg tgaacaacgt ggaggccatc
     1921 gcggcggtgg tgcccatcgt gaacgacgga ggagaggaat acgccaagat cggtatcgga
     1981 aagagcacgg gcaccaaact catcagtgcg gggggcaaca tcaagaggcc gggcgtgtac
     2041 gagatcgaac tgggcgtcag ttgcgaggag ttcctcaaca gcgatgaatg gtgcggcggt
     2101 gtggagggcc gcgcgctgaa ggccgtgatc cccggtggca gcagtgtgcc catcatcccg
     2161 gccgagctct acttcaagac cgccaagttc gagacccgcc tgatgaccta cgagagcctg
     2221 agcgatggcg gcttcgccac gggcaccatg ctcgggtccg gcggattcta cgcgtacaac
     2281 gacgcccagt gcatcgtgcg cagcacatgg aaccactcgc gcttttaccg gcacgagagc
     2341 tgtggccaat gcagcccctg ccgcgagggt acgggttgga tggagaagat cctctacaga
     2401 ctggaacatg gccagggccg cacggcggat attgacctgc tgtgggacat ccagcgacgc
     2461 atcgaaggca acaccatctg cccactcggt gatgcggcgg cttggcccgt ggcatccgcc
     2521 atccgccact tccgggacga gttcgagtac cacgccacgc accccaccaa ggtgaaggac
     2581 ccgaagcact tcgagaagga accgttcagg agggtggcga aggcggtggt gtaggacggg
     2641 gcttccttca cgccgcggcc aggccatgca cctcttccag cggcacgcat gcgatgcggt
     2701 cgtccagttc gtaacgacga gttccgggat agaccacata cagccggtcc agctggaggt
     2761 ccgccagggc cgtgcgcatg gaccgggtgg cgcgcggcgc gtcctcgcgc ttgaactcca
     2821 ccccggtgcg catgccgttg tgcaggaaga acaggtcgag ctccgatcca ctatgcacgg
     2881 cgtagaacca gacctgatgc ggcctgaagg cggcgagcgt ctcttccagt gcgaagcctt
     2941 cccaagaggc gcccaatttg gggtgggcga acagcgcggc cgtgtccttc acgccctgga
     3001 gcgcgtgaaa gagcccacta tcccgcaggt atattttggg gcttttcacc agccgcttgc
     3061 cgaggttctc gtaccacggc tgcagcgtgc gtaccatgaa ggtctgctcc agcgtgtcca
     3121 gccaggcgcg cgtggtgtgc gg
//
LOCUS       DAOQOC010000219         3133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15889_length_3133_cov_2.834633, whole genome shotgun sequence.
ACCESSION   DAOQOC010000219 DAOQOC010000000
VERSION     DAOQOC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_3133_cov_2.834633"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PL106_04665"
     CDS             complement(<1..474)
                     /locus_tag="PL106_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HNA32390.1"
                     /translation="MEGGKRIGSALISVYHKDGLEEIVRELHRLHVHLYSTGGTQEFI
                     EGLGVPVTPVEDLTGYPSILGGRVKTLHPKVFGGILGRRELASDVAQLEEYGIPPIDL
                     VIVDLYPFEATVAQGGSESGIIEKIDIGGISLIRAGAKNHTDVTIVASKAQYPELF"
     gene            complement(663..1340)
                     /locus_tag="PL106_04670"
     CDS             complement(663..1340)
                     /locus_tag="PL106_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32391.1"
                     /translation="MRPITLSLALCAALVSSAQLSGRGSLGGGIGLSIPTGDFRETLD
                     RNLFHVNAHLALPFKRVPILQSGFDFGYSVLGNYQRAVPVNTEYLDITEGLLTTRNKA
                     FSYHGFLRLSPLRGRVRPYVDGLLGLRQFRTTSKVTADGVEGHLSKEANQWDIAFSAG
                     WAAGLMVTFGKIAYVEGRVEHFNSGKATYVDPASVTINDQGTVNFSTLMSNTDVTNVT
                     VGIGLLF"
     gene            1546..1998
                     /locus_tag="PL106_04675"
     CDS             1546..1998
                     /locus_tag="PL106_04675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNA32392.1"
                     /translation="MEIGQGWELWGLWGLFLSSFVAATLLPFSSEALLGVMLLQGWPE
                     LPLLGVATAGNTLGGLVNYGIGRWVPDGKLARWIGMDPEKAERWRMLAHRHGAWAALG
                     CWLPVIGDPIALALGIFRTPLLSTAVLMAVGKAVRYAVVIGALGGTFT"
     gene            complement(2057..>3133)
                     /locus_tag="PL106_04680"
     CDS             complement(2057..>3133)
                     /locus_tag="PL106_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32393.1"
                     /translation="GKINAVGTAAKPIVFTSNEPPGQRNYGDWGGLIICGKAPNNAGA
                     NVLLEGGTGATFGGSLSDDNSGILRYVRIEFPGIAYQVNNEINGLTLGAVGSGTTMDH
                     IQVSYSGDDSYEWFGGSVNASHLVAFRGWDDDFDTDNGYAGRVQFGVALRDPAIADPS
                     GSNGVESDNDASGSSSAPVTSPIFSNISLYGPLTVDPNTGNMNTLFKRGAHLRRNTRL
                     SLYNSVIAGWPTDLLLDGGPTGDNVDAGLLQVRNTHLAAWGTIALDTVSGAGFDPIAW
                     FGTTGWGNAINSDLSSLGLPTPFSLAVPSFRPNGGSALLSGADFSSTNLVNPFFTPVS
                     YKGAFGGEDWTSGWCNWDPQNTVY"
BASE COUNT          588 a          955 c          969 g          621 t
ORIGIN      
        1 gaacagctcc gggtactggg cctttgacgc cacgatggtc acgtccgtat ggttcttcgc
       61 cccggcccgg atgagcgaaa taccacctat atcgatcttc tcgatgatcc cgctctcgct
      121 gccaccttgg gccaccgtgg cctcgaatgg ataaaggtcc acgatcacca ggtcgatggg
      181 tggaatacca tactcctcca gctgcgccac atcgctcgcc agttcacgcc gtcccaggat
      241 gcctccgaag actttcgggt gcaaggtttt cacccggccg cccaggatac tggggtaacc
      301 ggtgagatcc tccacgggtg tcaccggaac gcccagccct tcaatgaact cctgtgtgcc
      361 accggtgctg tacaggtgaa catggaggcg atgcaactcg cggacgatct cctcgagccc
      421 gtccttgtga taaaccgata tcagcgcact gccgatccgc ttgccgccct ccaccgtggt
      481 ttttgttcgt tgcgcaagtt tagaggccac gcaacggggg cttgggcgct ggatggtcgg
      541 cttttcagca gggttaccaa cactggacga tggaaaaggg catgtgtagg aacgccatgg
      601 aaacgatcga agtcacggaa tgcggaacca agttcgaacg atcacgaagc cctgaccgcc
      661 gatcagaaca gaagcccgat gcccacggtc acattggtga cgtccgtgtt gctcatgagc
      721 gtgctgaaat tcaccgtgcc ctggtcgttg atcgtgacgc tggccggatc gacataggtg
      781 gccttgccac tgttgaagtg ttccacccga ccttccacgt aggcgatctt gccaaaggtg
      841 accatcaagc ctgcggccca tcctgcgctg aacgcgatat cccattggtt ggcttccttg
      901 ctgaggtggc cttctacccc atcggccgtc actttgctgg tggtcctgaa ctgccgcaaa
      961 cccagcaggc cgtccacata aggtcgtacc cgaccgcgaa gcgggctcag ccgaaggaac
     1021 ccatggtagc tgaacgcctt gttgcgcgtg gtaagcaagc cctcggtgat atccaagtac
     1081 tccgtgttca ccggcacggc acgttggtag ttaccgagca cgctgtagcc gaagtcgaag
     1141 ccgctctgga ggatgggtac acgcttgaac ggcagggcca ggtgggcgtt cacatggaac
     1201 aggttgcggt caagggtctc cctgaagtcg ccggtgggga tgctcaggcc gatgccgccg
     1261 ccgagtgagc cacggccgct caactgggca ctggaaacca aggcggcaca gagggccagg
     1321 gagagggtga tcggacgcat ggacctgccg tttggcggac cactcccgat cgcgtgccga
     1381 tccgagttca gacacccaaa ataattcatg ccttggccat ccgatccaac cgggcttgct
     1441 ggatggccgc ctgatggaca ggctgaacag gtatggccag gatcaggcga caaatgcatc
     1501 catcctgcca tcggcgaacc cgcctccaca cccaacttcg ccccgatgga gatcggccaa
     1561 ggatgggaac tctggggact gtggggtctg ttcctgtcct cattcgttgc ggccactctg
     1621 ttgccgttca gttccgaagc gttgttggga gtcatgctgc tccaggggtg gcccgagctt
     1681 ccgttgctgg gtgtggccac cgctggcaat accctgggcg ggctggtcaa ttacggcatc
     1741 gggcgatggg tcccggatgg caagctggcc cggtggatcg gcatggatcc cgaaaaagcg
     1801 gaacggtggc gcatgctggc acaccgccat ggcgcgtggg cggccctggg gtgctggctc
     1861 cctgttatcg gggatcccat tgcgctggct ctcgggatct tccgcacacc actcttgtcc
     1921 accgcagtgc tgatggcggt aggcaaggcg gtacgctatg cggtggtcat cggcgcgctg
     1981 ggtggcacgt tcacctgatc accggtcatg gcggaaggcc catcccgggc cttccgcgcg
     2041 caacgatcac agggggtcag tacaccgtgt tctggggatc ccagttgcac cacccactcg
     2101 tccagtcctc gccgccgaag gctcccttgt agcttacagg tgtgaagaaa gggttgacaa
     2161 ggttcgtgct gctgaaatcc gcaccactga ggagggccga cccaccattc ggtcggaaac
     2221 tcggaaccgc caggctgaaa ggtgtgggca ggcccaggga cgagaggtcg ctgttgatcg
     2281 cgttgcccca gcccgttgta ccgaaccagg cgatcgggtc gaagcccgca ccggaaacgg
     2341 tatccaaggc gatggtgccc caagctgcga gatgggtatt gcgcacttga aggaggcctg
     2401 catccacatt gtcgccggta ggacctccgt ccaacaggag gtcggtgggc cagcccgcga
     2461 tcacggagtt gtacaggctc aggcgcgtgt tgcggcgcag atgtgcgccc cgcttgaaga
     2521 gcgtgttcat gttgcccgtg ttggggtcca cggtgagcgg tccgtacagg ctgatgttgc
     2581 tgaagatcgg gctcgtgacc ggtgcgctgg aggatccgct ggcgtcgttg tcgctctcga
     2641 cgccgttact accactcggg tcggcgatgg ccggatcgcg cagtgccaca ccgaattgga
     2701 cacgacctgc atacccgttg tcggtatcaa aatcatcgtc ccagccccgg aaggccacca
     2761 aatggctggc attcacacta cccccgaacc actcatagct gtcgtcgccg ctgtagctca
     2821 cctggatatg gtccatggtg gtgcccgagc ccaccgcacc gagggtaagg ccgttaatct
     2881 cgttgttcac ctgataggcg atacccggga actcgatgcg cacgtaccgc agtattccgc
     2941 tgttgtcgtc ggataagctg ccaccgaacg tcgcacctgt accaccttca agcagcacgt
     3001 tggcaccggc gttgttggga gccttaccgc aaatgatcag tccaccccag tcgccgtaat
     3061 tgcgctgacc cggaggttcg ttgctggtga agacgatggg cttggccgcg gtgccgaccg
     3121 cgttgatctt gcc
//
LOCUS       DAOQOC010000220         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_15941_length_3126_cov_3.794529, whole genome shotgun sequence.
ACCESSION   DAOQOC010000220 DAOQOC010000000
VERSION     DAOQOC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15941_length_3126_cov_3.794529"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PL106_04685"
     CDS             <1..942
                     /locus_tag="PL106_04685"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNA32394.1"
                     /translation="TTSRAGSDRADTMANTMRIGTHGRSLARASIPSVARIHALMRIE
                     GLVPVVDSSLAAQMAMAGVPVEPGTEVVDNPALAGIDLFFSLGGDGTILDTVAKTGQS
                     GIPVLGINLGRLGFLSSTREEGALAAIQAVKEGRYTIQERSLIALQGTDLANGETLIG
                     LNEMSVHKRDTSSMLVVDAWVNDRFLSTFWSDGLIVATPTGSTAYSLSCGGPILDPSC
                     DAMVITPISPHNLNVRPIVVPGASILRLHAEAREDKYLVNIDSRGFIVGRSLDLHVRK
                     AAHRARFVQLEGTGFFNTLREKLGWGMDVRSAKMSER"
     gene            1019..1861
                     /locus_tag="PL106_04690"
     CDS             1019..1861
                     /locus_tag="PL106_04690"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNA32395.1"
                     /translation="MPRHSLVVLSLMVALAPASAQVSEAGLTGGLSYYIGDINPLRHY
                     PRHMHMAGGLFYRYNITPHYAVRFQGLYTRLEAYDSDSPDPVQQARNLGFRSNIFELA
                     FTVEVNFLKYRSIDRKNNHLWTPYLFVGLGYFHMNPQNRLDDTWYDLQPLGTEGQGTA
                     SGGKPYPLDVFNIPHGFGFKFALSDRVDLGVEWGLRRTYTDHLDDVGGVYADRGVLVE
                     EAGPLTAALADPSAARTSGINTGRARGDSNTKDWYQYTGLSLSMLLTRFTECDAMWSK
                     RKHR"
     gene            1989..2765
                     /locus_tag="PL106_04695"
     CDS             1989..2765
                     /locus_tag="PL106_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNA32396.1"
                     /translation="MSAHAKISTAEELRSRIDPARVPEHVAIIMDGNGRWAQRQGEHR
                     VVGHSNGVKSVRDTLVGATEIGVRYLTFYAFSTENWNRPQAEVDALMDLLVNTLMSEL
                     DSLNENGVRLHTIGDTDSLPASCRNTLQEAIRRTAHNTRITLTLALSYSARWEIVRAA
                     RSIAQDVQEGRLAPSAVDEALVSSRLSTAGMPDPELLIRTSGEQRVSNYLLWQIAYAE
                     LWFTAVLWPDFRKEHLFQAVLDYQGRERRFGLTSEQLDPA"
     gene            2762..>3126
                     /locus_tag="PL106_04700"
     CDS             2762..>3126
                     /locus_tag="PL106_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32397.1"
                     /translation="MSRTLRSILRVILGTALLLGAIRGAHAQTISGPVLDYAYPKEYT
                     IGGIQVFGCVTRDTKAVELFSGLQVGERITVPGERIGKAIQAIWDQHLFTDVRIEAAR
                     ITGTTIFLNIIVQENPTVRG"
BASE COUNT          602 a         1039 c          974 g          511 t
ORIGIN      
        1 acaacaagca gggcgggaag cgaccgggcg gacacaatgg cgaacacgat gcgcatcggc
       61 acgcacggga gatcactcgc ccgggccagc atcccctcgg tggcgcggat ccacgcgttg
      121 atgcgcatcg aaggcctggt acccgtggtg gacagcagcc tggcggcaca aatggccatg
      181 gccggcgtgc ccgtggaacc cggcaccgaa gtggtggaca acccggcgct cgcgggcatc
      241 gaccttttct tcagcctggg cggcgatggc accatcctgg acaccgtggc gaaaaccggt
      301 cagagcggca ttccggtgct gggcatcaac ttggggcggc tcggtttcct cagcagcacg
      361 cgcgaagaag gtgcgctcgc cgccatccag gccgtgaagg agggccgata caccatccag
      421 gaacgcagcc ttatcgccct gcagggcacc gacctggcca atggcgaaac actcatcggc
      481 ctcaacgaga tgagcgtaca caagcgcgac accagcagta tgctcgtggt ggacgcctgg
      541 gtgaacgacc gcttcctgag caccttctgg agcgacggcc tcattgtggc cacgcccacg
      601 ggcagcaccg cttactcatt gagctgcggc ggccccatcc tcgatccctc ctgcgacgcc
      661 atggtgatca cacccatctc gccccataac ctcaatgtgc ggcccatcgt ggtgccgggc
      721 gccagcattc tgcggctgca tgccgaagcc cgcgaggaca agtacctggt gaacatcgac
      781 tcgcggggct tcattgtagg ccgctcactg gatctgcacg tgcgcaaggc cgctcaccgt
      841 gcccgtttcg tgcaactcga aggcaccggc ttcttcaaca cgctgcgcga aaaactgggc
      901 tggggcatgg acgtgcggag cgcaaagatg agcgaacgtt gaggggcgcc cccaaggaag
      961 aggctaagtt cgtggcctca aaggcaacag tccgcgtgtt gttgcgttga agccccgcat
     1021 gcctcgtcat tccctggtcg ttctcagcct catggtggcc ctggcgcccg cttcggccca
     1081 agtgagcgaa gcgggcctta ccggggggct cagctactac atcggggaca tcaacccctt
     1141 gcggcactat ccgcgccaca tgcacatggc cggtgggctt ttttaccggt acaacatcac
     1201 gccgcactat gctgtgcgct tccaaggcct gtacacccgc ctggaagcgt acgactccga
     1261 ttcgcccgat cccgtgcagc aggcgcgcaa cctgggcttc cgctccaaca tcttcgagct
     1321 ggccttcacc gtggaggtca acttcctgaa ataccgcagc atcgaccgga agaacaacca
     1381 cttgtggacg ccctatctct tcgtaggtct gggctacttc cacatgaacc cgcagaaccg
     1441 gctggacgac acctggtacg atctgcagcc cttgggaacc gagggacaag gcaccgcatc
     1501 gggtggcaag ccgtaccccc tcgatgtctt caacatcccg cacggcttcg gtttcaaatt
     1561 cgcgctgtcc gacagggtgg acctgggcgt ggaatggggc ttgcgccgca cgtacaccga
     1621 ccacttggat gatgtgggcg gggtctatgc cgaccggggc gtcctggtcg aagaggctgg
     1681 cccgctcacc gcagcccttg ccgaccccag cgcggcgcgt acgtcgggca tcaacaccgg
     1741 ccgcgcccgg ggcgacagca ataccaagga ctggtaccag tataccggcc tgagcctttc
     1801 catgctgctc acccgcttca cggagtgtga tgccatgtgg agcaaacgga agcaccggta
     1861 gggggttgga gcagtccgtg acccggcgcg gtccggcaag tacacacgta ctggtgggga
     1921 tcaggcgcga aaaccgcaaa ttcgcgcccc gttcgccgga gtgagcacaa aggcgggcca
     1981 ggtgacccat gtccgcccac gccaagatca gcacagccga agaattgcgc tcccggatcg
     2041 atccggcccg tgtgcccgag catgtggcca tcatcatgga cggcaacggc cgctgggccc
     2101 agcgccaggg cgagcaccgc gtggtgggcc acagcaacgg ggtgaagagc gtgcgcgaca
     2161 cgctggtggg ggccacggag atcggtgtac gatacctcac cttctacgcg ttcagcaccg
     2221 agaactggaa ccggccgcag gccgaagtgg acgcgctgat ggacctgctg gtgaacacgc
     2281 tgatgagcga attggacagc ctgaacgaga acggcgttcg cctgcacacc atcggcgata
     2341 cggacagcct gcccgcgagc tgccggaaca ccttgcaaga ggccatccgt cgcaccgccc
     2401 acaacacacg catcacgctc accctggcac tcagctacag cgcgcgctgg gagatcgtac
     2461 gcgcggcccg ttccattgcg caggatgtgc aggaaggccg cctggcccct tcggccgtgg
     2521 atgaggcgct ggtgagttcc cgcctgagca ccgcgggcat gcccgacccc gaattgctga
     2581 tccgcaccag cggcgagcag cgcgtgagca actacctcct ctggcagatc gcctatgccg
     2641 agctctggtt caccgccgtg ctctggcccg atttccgcaa ggagcacctg ttccaggccg
     2701 tactcgacta ccagggccgc gaacgtcgct tcggtcttac cagcgagcaa ctcgatcccg
     2761 catgagccgg accctgcgct ccatcctgcg cgtgatcctg ggcacggcac tcctgctggg
     2821 ggccatccgt ggcgcgcatg cgcagacgat cagcgggccc gtgctcgatt acgcttatcc
     2881 gaaggaatat accatcggcg gtatacaggt cttcggctgc gttacgcggg ataccaaagc
     2941 ggtggaactt ttcagtggcc tgcaggtggg cgaacggatc accgtgccgg gcgagcgcat
     3001 cggcaaggcc atccaggcca tctgggatca gcacctgttc accgatgtgc ggatcgaggc
     3061 cgcgcgcatc acgggaacca ccatcttcct gaacatcatc gtgcaggaga acccgaccgt
     3121 ccgggg
//
LOCUS       DAOQOC010000221         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16037_length_3116_cov_3.389089, whole genome shotgun sequence.
ACCESSION   DAOQOC010000221 DAOQOC010000000
VERSION     DAOQOC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16037_length_3116_cov_3.389089"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="PL106_04705"
     CDS             complement(<1..1674)
                     /locus_tag="PL106_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32398.1"
                     /translation="MNRRLDSCNTSGQGSWWRKITAVMAVLTLFVSELSAQSYAVIGT
                     GTASNTATSNSPVNIYYRRTVCQMVYTASELSAAGIGVGATISELGWYVTGAPLYAMP
                     DYTIKFKHVTATNVAAALGTTGWTTVKNAFSYQPPAGGWNMITLDNNFVWDGSSNLGV
                     EVCWSQVFPGFNASGQCYIYTVTNGYRYSRTDAAGTSCGLSPATATTTKPQVRLGWVG
                     SGTCTGTPAPGNTTGPSVVTSGTTISLGLQNPTMGNDVTYQWYVSTSSSSGPWTPTGT
                     STASLSATVTQDSWFYCAVTCPEGPSTGNSNVLHVEVVPVVLVPFTGSNTNACGTNIA
                     LQDHAGSGNYSNNANGYTVLEAGTNATITISGTYWGEGCCDDLYIYSGAGTGGAVLLN
                     PIGNGAGGVSVNYTGAPGQTLTVRFTSDLSIVYAGFDLMVTYTNPCVPPCIPVATASL
                     DEDCGNSQFYVDVEVTDLNGAPSVSINSDYSGDPGAVSAVGVGSYSIGPFASTSTVNI
                     TVTNDGGGPGCETVLGPYTMDCALLGKNALDFDGADDRVNLGDPASLQIT"
     gene            complement(2385..>3116)
                     /locus_tag="PL106_04710"
     CDS             complement(2385..>3116)
                     /locus_tag="PL106_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNA32399.1"
                     /translation="LSLGIETMGGVMTKLIEANTTIPTKKSQTFSTASDSQPSVEIHV
                     LQGERPMAAGNRTIGRFHLDGIPPAPRGTPQIEVTFDIDANGILHVGAKDKTTGKEQS
                     IRIEASSGLNKDDIEKMKREASANADADKAARERADKLNQADSMVFQMEKNMKEYGDK
                     LPADKKAPIEEAITKLKTAHAAQDIPAIDTAMAELNNLFNAASQEMYAAAQQAQGANP
                     GTEPKAGQNKDTEVTDVDFEEVKNP"
BASE COUNT          527 a          911 c          967 g          711 t
ORIGIN      
        1 cgtgatctgc agggaggcag gatcgcccag gttcacccgg tcatccgccc cgtcgaagtc
       61 caaggcgttc ttgcccagca gcgcgcaatc catggtgtag gggcccaata ccgtttcaca
      121 tccgggcccg ccgccgtcat tcgtcaccgt gatgttcacg gtgctcgtgc tggcgaacgg
      181 tccgatcgaa tagctgccga cccccactgc ggataccgca cccggatccc ctgaatagtc
      241 gctgttgatg ctcacgctcg gcgccccgtt cagatcggtc acctccacat ccacatagaa
      301 ctggctgttt ccgcaatcct cgtccaagga tgcggtggcc accggtatgc atggaggtac
      361 gcaggggttg gtgtacgtga ccatgagatc gaatccggcg tagacgatgg agaggtccga
      421 tgtgaatcgt accgtaaggg tctgtcccgg tgctccggta tagttgacgc tcaccccgcc
      481 ggcgccgttc ccgatcgggt tcagcagcac ggcacctccg gtccccgctc cactatagat
      541 gtacaggtcg tcgcagcagc cctcgcccca gtacgtaccg gaaatggtga tggtggcatt
      601 ggtgcccgcc tccagcacgg tgtacccgtt ggcgttgttg ctgtagttgc cggatccggc
      661 gtggtcctgc aatgcgatgt tggtgccgca tgcattggta ttggaaccgg tgaacggtac
      721 gagcaccaca ggcaccacct ccacatggag tacgttgcta ttgcccgtgc tcggcccttc
      781 cgggcaagtg accgcacagt agaaccagct gtcctgcgtg accgttgcgg acaagctggc
      841 cgtactggtt ccggtgggtg tccatggacc cgaagagctg ctcgtgctca cgtaccattg
      901 gtaggtgacg tcattgccca tggtcggatt ctgcaacccc aggctgatgg tagtgccgct
      961 ggtgaccacc gaaggaccgg tggtgttgcc cggggccggt gtgccggtac aggttccgct
     1021 gcccacccac ccgagacgta cctggggttt ggtggtggtc gccgtggccg gtgagagtcc
     1081 gcaagaggtc cctgctgcat cggtacggct gtaacgataa ccgttggtga ccgtgtagat
     1141 gtagcactgg ccgctcgcat tgaagcctgg aaatacctgg gaccagcaga cctccacccc
     1201 caggttggaa ctgccatccc aaacgaagtt gttgtccagc gtgatcatgt tccaaccacc
     1261 cgcaggaggc tggtagctga atgcgttctt caccgtggtc cagcccgtgg tgcccaaggc
     1321 cgcagccacg ttggtcgccg taacgtgttt gaacttgatc gtgtaatccg gcatcgcgta
     1381 gagcggtgct ccggtcacgt accagcccag ttcggagatc gtggcaccta ccccgatgcc
     1441 ggccgcactc agttcgctcg ccgtgtacac catctggcac accgtgcggc ggtagtaaat
     1501 gttcaccggt gagttggacg tggcggtgtt gctcgccgtt ccagtgccga tcaccgcata
     1561 gctctgggca gagagctctg atacgaacag ggtcaacacg gccatcacgg ccgtgatctt
     1621 cctccaccag gatccttgtc ctgatgtgtt gcaggagtcg agtcttcggt tcatgggctt
     1681 gggattgagg tttctgagat ggggaaaggc cgggtcgtag gtggttggcg cgagtgaact
     1741 gaccttcggt cggttcatcc ggtttttggg tcggggcatg tgccaaagac atggcacttg
     1801 ctcgtggcga tcggatccgg aaacaaccat ccatcacccc agcgatggtg tgaagaatgg
     1861 atcatgctgg acgcctccga agcgtcaacg taccagcggg aggggtggca cctggccatg
     1921 gtcgccgggg aggatcggaa aggagcagga ggtcgcttag agccatgcaa gcttatggag
     1981 atgatcgatg cagcggtact attttttgaa aattgttaat ttctttcata ggcaaccata
     2041 gcgcgttcgt cgcgtacagg tcggggttcg tcgatgatct tgacgcatgg ctgtactcgg
     2101 ccgagaccca ttcctgacgg cccatccaga ctattcggat cgaggtctga tatcattttg
     2161 cggtaggggg ccatccaaaa tgtcacgctg agcccgtcga cggttcggca agctcaccgc
     2221 ttcgacgggc tcagcgtgac ataccatggt ccgatcggac gatgctgtat tgcgaaatgg
     2281 tatgaaagcc gaagagcgca tcccgatcgg tatgggtcat gaaatacgga agagccgccc
     2341 cggatgcccg ggacggctct tttcgtgcct gtctggttgg acccttacgg gttcttcacc
     2401 tcttcaaagt ccacatcggt gacctcggta tccttgttct gtccggcctt gggttcagta
     2461 ccggggttgg cgccctgcgc ttgctgagca gcggcgtaca tttcttggct ggccgcgttg
     2521 aacaagttgt tcaattccgc catcgcggta tcaatggccg ggatgtcctg cgcggcatgg
     2581 gccgttttga gcttggtgat agcctcctcg atgggggcct tcttgtccgc gggcagctta
     2641 tcgccgtact ccttcatgtt cttctccatc tggaagacca tgctgtcggc ctggttgagc
     2701 ttgtccgcac gctcgcgcgc ggccttgtcg gcatcggcgt tggcgctggc ctcccgcttc
     2761 atcttctcga tgtcgtcctt gttcaggccg ctgctcgcct cgatgcggat gctctgctcc
     2821 ttaccggtgg tcttgtcttt ggcacccaca tgcaggatgc cgttcgcgtc gatgtcgaag
     2881 gtgacctcga tctgcggcgt tccgcgcggg gcgggcggga tcccatcgag gtggaaccgc
     2941 ccgatggtgc ggttgccggc cgccatgggg cgctctcctt gcaatacatg gatctccacg
     3001 ctcggctggc tatcgctggc cgtgctgaag gtctggctct tcttggtggg gatggtggtg
     3061 ttggcctcga tcaatttggt catcacaccg cccatggtct cgatgcccag gctcag
//
LOCUS       DAOQOC010000222         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16114_length_3108_cov_3.995414, whole genome shotgun sequence.
ACCESSION   DAOQOC010000222 DAOQOC010000000
VERSION     DAOQOC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16114_length_3108_cov_3.995414"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PL106_04715"
     CDS             <1..235
                     /locus_tag="PL106_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32400.1"
                     /translation="DVTFNGQVKYTGGGNDRDPVLTTVGSATPGNTVNAYSTRDVNLN
                     GQVKYTGSSNDRDPILLNVGSATPNNIRSAQLP"
     gene            442..>3108
                     /locus_tag="PL106_04720"
     CDS             442..>3108
                     /locus_tag="PL106_04720"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32401.1"
                     /translation="MKRSFAIAAACISAIAATAQLEQNRYSADPHGLPDWVQLMYAPD
                     ADPGAVQAAYTAYYATHPFVKNSHTQYFKRWKRELGHALVPRDPGQLAQYEQTSRAYL
                     DATGALLGDRAANWSCIGPFDWDHGAVAKSYACGAAHVYTVEQANSDGNTVYAGTANA
                     GVWKSTDKGLNWTNMTKDMLVGEVLSVEIDHTNANVVWFGGAGKLYKTTNGGTTWSTA
                     GDAAFNAVSHTIRDIVLHPTNNQKLFVCSNQGLYRSDNGGTTFTQVQAGTWQELEFKP
                     GDPTYVYAVKQTGIVTQFWRSTNSGSTFTQMTTGWPVAVAPSEQERTEIAVSAAAPNM
                     VYALCTGVADGGSGLYGVYRSTDSGASWTFQCCGTGPGGVPSPTNFNLMGWDDGGQDD
                     GGQYYYDLAFAVDPTNASKVNVCAVNRWVSTDGGVNFTCPAKWSHSNKVDYLHADIHD
                     IRYYGGSEIWAACDGGVFYSNDGGATFNRRMFGIAGTDFWGFGVGGWTGSQVMLGGTY
                     HNGTLLKDNNVYTNGWVSTDGGDNYRGFVHPQYDRRALSDYGYKVLSGDRTVNNTNGS
                     WGKQPNADYTTGHSSEVVWHPNLVNTAYVGNGNSIWRTDDNGVSFVEVYNFGEYVTSI
                     EVAFSDPNIMYACTYVDWWGAKKVWKSTTAGTSWTDVTPTPAQLNGNEWVPYDIAVSA
                     TDPQTIWLVRTSQYGDYPDHDGYVVFRSINGGTSWTNITSPALNGEWPTGIAHQIGSN
                     GGLYIGTRRSVYYRSDATGSWGLWNAGLPARIYSTRLLINYRDQKIRNGTDRSVWESA
                     LESPAPPVANFAADKRALSCLAPTVQFWDNSALANTAATWSWSFPGGSPATASVRSPV
                     VTYSTPGTFDVSLTVTDANGTNTRTIPGFITVS"
BASE COUNT          649 a          946 c         1004 g          509 t
ORIGIN      
        1 cgatgtcacg ttcaacggcc aagtgaagta cacgggtggt ggcaacgatc gcgatccggt
       61 cctgaccacg gtgggcagcg ctacgcccgg caacacggtg aacgcgtaca gcacccgcga
      121 cgtgaacctg aacggtcagg tgaagtacac gggcagcagc aacgaccgtg atccgatcct
      181 gttgaacgtg ggcagcgcca cgccgaacaa catccggtcg gcacagttgc cgtaagccgg
      241 ttcaataggt cagcgaaggg ccccggtcat cgaccgtggc ccttcgcatt tccgcgcatg
      301 cagggcgctc catggatgtc actgcggcca cccgatggac aggagttgag cgatgggcac
      361 tggatggatc accgatcttc cgcacgtccc gttgggggct ccggccttgc gggtattttc
      421 gccacaacaa gaatgtgagc aatgaaaagg tccttcgcga tcgcggccgc gtgcatatct
      481 gccattgcag ccacagccca actggaacag aaccggtaca gtgccgatcc gcacgggctg
      541 cccgactggg tgcaactgat gtacgcgcct gatgccgatc cgggtgcggt acaagcggcg
      601 tacaccgcgt actacgccac gcacccgttc gtgaagaaca gccacacgca gtatttcaag
      661 cggtggaaac gggaactggg tcatgcattg gtgcccaggg accctggtca gctggcgcag
      721 tatgaacaga cctcacgcgc ctacctcgat gcgaccggag cgctgcttgg cgatcgtgcc
      781 gcgaactggt cgtgcatcgg gccgttcgac tgggatcatg gtgcggtggc caagagctac
      841 gcctgcgggg ccgcgcacgt gtacacggtg gagcaggcca attccgatgg caatacggtg
      901 tatgcgggaa cggcgaacgc cggcgtgtgg aagagcacgg acaaggggct gaactggacg
      961 aacatgacca aggacatgct cgtgggcgaa gtgctcagcg tggagatcga tcacaccaat
     1021 gcgaacgttg tatggttcgg tggtgcgggg aagctgtaca agaccacgaa cggcggtacg
     1081 acctggtcca ccgccggtga tgcggccttc aatgcggtat cccacaccat ccgcgacatc
     1141 gtgctgcacc ccacgaacaa ccagaagcta ttcgtttgca gcaaccaggg cctgtaccgc
     1201 agcgacaacg gcggcactac cttcacccag gtacaggcgg gcacatggca ggagctcgag
     1261 ttcaagcccg gcgacccgac gtacgtgtac gcggtgaagc agaccggcat cgtcacgcag
     1321 ttctggcgca gtaccaacag cgggagcacg ttcacgcaga tgaccacggg ctggccggtc
     1381 gccgtggcac cctcggagca ggaacgcacc gagatcgcgg tgagcgcggc ggcgcccaac
     1441 atggtgtacg ccctgtgtac cggtgtggcg gatggaggga gcgggctgta cggagtgtac
     1501 cggagtaccg acagtggcgc gagctggacc ttccaatgtt gcggcaccgg ccccggtggt
     1561 gtgccctcac cgaccaactt caacctgatg ggctgggacg atggcggcca ggacgatggc
     1621 ggacaatact actatgatct cgctttcgcg gtagacccaa cgaatgcctc caaggtcaac
     1681 gtgtgcgcgg tgaaccgctg ggtgagcacg gatggcgggg tcaatttcac ctgcccggcg
     1741 aagtggagcc acagcaacaa ggtggactac ctgcatgcgg atatccatga catccgctac
     1801 tatggcgggt cggagatctg ggcggcgtgt gatggcggtg tcttctacag caacgatgga
     1861 ggggccacct tcaaccggcg catgttcggc atcgcgggta cggacttctg gggcttcggt
     1921 gtcggcggct ggaccggcag ccaggtgatg ctcgggggta cctatcacaa cgggaccctg
     1981 ctcaaggaca acaacgtgta caccaatggc tgggtgagca cggacggtgg cgacaactac
     2041 cggggcttcg tacatcccca gtacgatcgc cgggccttga gcgactacgg ttacaaggtg
     2101 ctgagcggcg accgcacggt gaacaacacc aacggctcgt ggggcaagca acccaatgcc
     2161 gattacacca ccgggcacag cagcgaagtg gtgtggcatc cgaacctggt gaacaccgcg
     2221 tacgtgggca acggcaattc gatctggcgc accgacgaca acggggtctc cttcgtggag
     2281 gtgtacaact tcggggagta cgtcacgagt atcgaggtgg ccttcagcga tcccaacatc
     2341 atgtacgcct gcacgtatgt ggattggtgg ggagctaaga aggtctggaa aagcaccacc
     2401 gctggcacca gttggaccga cgtcacgccg acgcccgcgc agctcaacgg caatgaatgg
     2461 gtgccgtacg acatcgcggt gagcgcgacg gacccccaaa cgatctggct ggtgcgcacc
     2521 tcgcagtacg gcgactaccc ggaccatgat ggctacgtcg ttttccgcag catcaacggt
     2581 ggcacgagct ggacgaacat cacctcgccc gcgttgaacg gcgaatggcc cacgggcatc
     2641 gcgcaccaga tcgggagtaa cggtggattg tacatcggta cacggcgttc ggtatactat
     2701 cgcagcgacg cgaccggctc atggggcttg tggaacgccg gcctgcccgc tcgtatctac
     2761 agcacccgac tgctcatcaa ctaccgcgac cagaagatcc gcaacggaac ggaccgcagc
     2821 gtgtgggaga gcgccctcga gtccccggca ccgccggtgg ccaatttcgc cgccgacaag
     2881 cgcgcgttga gctgcctcgc gccgacggtg cagttctggg acaacagtgc gctggccaac
     2941 accgccgcca cctggagttg gagcttcccc ggagggagcc cggcaacggc atcggtgcgc
     3001 agcccagtgg tgacctactc cacgcccggt acattcgacg tgtcgctcac cgttacagat
     3061 gcgaacggta cgaacacgcg cacgatcccc ggtttcatca ccgtttcc
//
LOCUS       DAOQOC010000223         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16170_length_3103_cov_2.632546, whole genome shotgun sequence.
ACCESSION   DAOQOC010000223 DAOQOC010000000
VERSION     DAOQOC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16170_length_3103_cov_2.632546"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PL106_04725"
     CDS             <1..366
                     /locus_tag="PL106_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32402.1"
                     /translation="VPVGSDAKLTPGDRLTIRIELRTDRPVDHVHLKDLRGAGLEPTE
                     TLSGYRYQDGLGYYRTTRDASVNFFFDRIMPGTYVLEYDLNVSHAGDFSNGITTAMCM
                     YAPEFSSHSQGVRVRVEGR"
     gene            complement(443..742)
                     /locus_tag="PL106_04730"
     CDS             complement(443..742)
                     /locus_tag="PL106_04730"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA32403.1"
                     /translation="MILTITAQARERLFGIWLHIRETASEERADKVEERLLKRAQELI
                     EQPWKGPPERELVGLNKGHRFLLLGRYKIIYRVVGDKIHITDFFDTKRHPSHMRG"
     gene            complement(739..969)
                     /locus_tag="PL106_04735"
     CDS             complement(739..969)
                     /locus_tag="PL106_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32404.1"
                     /translation="MNTLGIRSEVKRLIDREKDPSVLELIKSILSAGPLNKEMAEDMM
                     RVALLSDEDIAAGRTHTVEEVRRWMAERKRKA"
     gene            1111..1713
                     /locus_tag="PL106_04740"
     CDS             1111..1713
                     /locus_tag="PL106_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNA32405.1"
                     /translation="MRNTLLVALVALTLDAAAQSPTAWRDSLTAYWARLDAEFADSAT
                     SPLTAGDRALFQHLEHFPFDSTYHLVGLFVPATDTTSFAMRTTRTRTPMYRTYGTLRF
                     TLNGAERTLSVYQSVPPHPGYEDMLFVPFTDLTNGAETYGVGRYLDLSLPMDSTVTLD
                     FNRAYNPYCAYNDRYSCPVPPSGNHLATAVRAGVLNFREH"
     gene            1741..2256
                     /locus_tag="PL106_04745"
     CDS             1741..2256
                     /locus_tag="PL106_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNA32406.1"
                     /translation="MNDQSLINKVAASGIITLDLEDLYPQGERIVFDLAPLLWQGIAL
                     KESDLRTFVKEHDWSRYANAYVAVHCSVDAIIPTWAYMLVATHLQPVAAFITQGDAEQ
                     LERAIFTRFIHDLDTAPYSGGRVVVKGCSALPVPLNAYVELGTRLLPVVKSLMFGEPC
                     STVPLYKAPKQ"
     gene            2272..>3103
                     /locus_tag="PL106_04750"
     CDS             2272..>3103
                     /locus_tag="PL106_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32407.1"
                     /translation="MKPIARLLFLALCWWSVPVSHAQTDTATVPHSDNGWYLSPHDTI
                     RVLVLFCEIEYDQNPGKNPANDAAEHWPKGQLPKWKDDLFDPMPKSAPTAMVTRYYHD
                     VSLGNYIVLGDYLDVMLTLPESEYPEVKSAHSIGKAAVKEANKLGQLRTKHGLRIEDF
                     DLWKDEGRPGMPKEHGPDDPHRYDHVMVIARNSGLTHGQGSTDAGSPGPLFGYESDTQ
                     SRFGGMNALPFEILKHEYNHLLLGGNNFHSGGGNAAQFDSYTLCMQGGWSLMGAASSS
                     L"
BASE COUNT          602 a         1035 c          910 g          556 t
ORIGIN      
        1 gttcccgtgg gcagcgatgc gaagctgacg cccggtgacc gcctcaccat ccgcatcgag
       61 ctacgcaccg atcgcccggt ggaccacgta cacctgaagg acctgcgtgg tgcgggactg
      121 gaacccaccg agaccctcag cggctaccgc taccaggacg gcctcgggta ctaccgcacc
      181 actcgcgatg ccagcgtcaa cttcttcttc gaccggatca tgccgggcac ctacgtactg
      241 gagtatgatc tgaacgtgag ccacgcgggc gacttcagca acgggatcac cacggccatg
      301 tgcatgtacg cgcccgagtt cagcagccac agccagggag tgagggtccg tgtggaaggc
      361 cggtagagaa gacggccgaa gacaagctgc gccctgttct ggcgcgaagg tggcccaaac
      421 acgcgctttc cctgcaccgt gctcacccgc gcatgtgcga ggggtgccgc ttggtgtcga
      481 agaaatcggt gatgtggatc ttgtccccga ccaccctgta gatgatcttg taccgaccaa
      541 gcaggagaaa acgatgacct ttgttcaggc ccaccaattc ccgctcaggc ggccctttcc
      601 atggctgttc gatcagttcc tgggcccgtt tcaacaaccg ctcttccacc ttgtccgccc
      661 gctcctccga agcggtctcc cggatgtgca accagatccc gaatagccgt tcgcgggcct
      721 gggccgtgat cgtcaggatc atgccttgcg cttccgctcg gccatccagc gccgcacctc
      781 ttcaacggta tgggtcctgc ccgcggcgat atcctcgtcg ctgagcaggg ccacgcgcat
      841 catgtcctcg gccatttcct tgttcaaggg gcctgctgaa aggatgcttt tgatcaattc
      901 aaggacggag gggtccttct cgcggtcgat cagccgtttc acttcggaac ggataccaag
      961 cgtattcatg cgacttgcgc tttcgccaaa ggtacggcca taggggagcg gttccgggtg
     1021 cggccttcgt tccgggacag acacggatgt acgcatgggc gttctcgccg cacagcgaag
     1081 gcgtacgttt gaggatcaat ggcgaatagg atgaggaaca cgctgctggt cgcactcgtg
     1141 gcgctgaccc tggatgcggc ggcgcaatct ccaacggcct ggcgcgacag cctgaccgcc
     1201 tactgggcac ggcttgatgc cgagttcgcc gacagcgcca cctccccgtt gaccgccggg
     1261 gaccgggcgc tgttccagca tctcgaacac ttccccttcg acagcaccta ccatttggtc
     1321 gggctgttcg tccccgccac ggacaccacc tccttcgcca tgcgtaccac gaggacgcgc
     1381 accccgatgt accgaacata cggtaccctg cgcttcaccc tgaacggtgc ggagcgcaca
     1441 ctgagcgtgt accagagcgt gccaccacat cccggctatg aggacatgct cttcgtgccc
     1501 ttcaccgatc tcaccaatgg cgccgagacc tacggggtgg gccgttacct cgacctgtcg
     1561 ctgccgatgg acagcaccgt caccctcgac ttcaaccggg cctacaaccc gtattgcgcg
     1621 tacaacgacc ggtacagctg ccccgttccg cccagcggga accacctggc caccgcggtg
     1681 cgggcggggg tgctgaactt tcgggaacac tgatcgatca ccgatctttg cggcaccctc
     1741 atgaacgacc agtccctcat caacaaggtc gccgcatccg gcatcatcac actcgatctg
     1801 gaggatctct acccgcaagg cgagcgcatc gtgttcgacc tggcaccgct gctctggcag
     1861 ggcatcgcat tgaaagagtc cgacctgcgc acattcgtga aggagcatga ctggtcgcgc
     1921 tacgcgaacg catacgtggc tgtgcattgc tcggtggacg cgatcatccc cacctgggcg
     1981 tacatgctgg tggccacgca tcttcaaccc gtggccgcct tcatcaccca aggtgacgcg
     2041 gaacaactcg agcgcgccat cttcacgcgg ttcatccacg acctggatac agccccgtac
     2101 tcgggtggcc gggtggtggt gaaaggatgc agcgctctcc cggtcccgtt gaacgcatac
     2161 gtggaactcg gtacccgttt gctccccgtg gtgaaaagcc tgatgttcgg cgaaccgtgc
     2221 agcacggttc cgctgtacaa ggcaccgaaa cagtagccct accttggcgc gatgaagcct
     2281 atcgcgcgcc tgttgttcct cgccctgtgc tggtggtccg tacctgtcag ccatgcccaa
     2341 acggacacgg ccactgttcc gcacagcgac aacggttggt acctgagccc gcacgacacc
     2401 atccgggtcc tggtcctctt ctgcgagatc gagtacgacc agaaccccgg gaagaaccca
     2461 gcgaacgacg ctgccgaaca ctggcccaag ggccagctac cgaaatggaa ggatgatctg
     2521 ttcgatccca tgccgaagtc cgcgcccacc gcgatggtca cccgttacta ccacgacgtc
     2581 tcgctcggca actatatcgt tctcggcgac tatctggatg tgatgcttac cctgcccgaa
     2641 agtgagtacc ccgaggtgaa gagcgcgcac agcatcggca aggccgccgt gaaggaggcc
     2701 aacaagctcg ggcagctgcg tacgaagcac ggcctgcgca tcgaggactt cgacctgtgg
     2761 aaggacgagg ggcggccggg catgccgaaa gaacacgggc cggatgatcc gcaccgctac
     2821 gaccacgtga tggtgatcgc gcgcaacagt gggctcacgc acggacaagg cagcacggac
     2881 gcgggaagtc cgggcccgct gttcggctat gagagcgaca cccagagccg cttcggtggc
     2941 atgaacgccc tgccgttcga gatcctcaaa cacgagtaca accacctgct tctcggcggc
     3001 aacaacttcc acagcggggg cggcaacgcc gcccagttcg acagttacac cctgtgcatg
     3061 cagggcggct ggagcctgat gggcgcggcc agcagcagct tgc
//
LOCUS       DAOQOC010000224         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16181_length_3102_cov_3.513292, whole genome shotgun sequence.
ACCESSION   DAOQOC010000224 DAOQOC010000000
VERSION     DAOQOC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16181_length_3102_cov_3.513292"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PL106_04755"
     CDS             complement(<1..863)
                     /locus_tag="PL106_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNA32408.1"
                     /translation="MRALLALNPYFRRYWGRMALGILFTALSSLFQIFQPQVVREAFD
                     LIGTSLAQRDQPLDQRHLDVPAEINSWIGWTGIDLDARLNGAATDEDRRTNVRWFAAF
                     YALLFVAFTLLQGFFMFLMRQTIIVVSRLIEYDLKNDIFAHYQRLDRGFYKGHSTGDL
                     MNRISEDVGKVRMYIGPAIMYILGMVVLSVMIVWVMVGVNVKLTLWVLAPLPLMSIAI
                     YYVSDLMNKRGLAVQAQQSVLSSLAQESFSGIRVIKSFAREKLVFGRFRDAAHTYRER
                     SLDQARVDAIF"
     gene            1158..3041
                     /gene="rpsA"
                     /locus_tag="PL106_04760"
     CDS             1158..3041
                     /gene="rpsA"
                     /locus_tag="PL106_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNA32409.1"
                     /translation="MSAEQTKVTFAEPPADFDWATLEDDKKSISASDRERMEKAYEES
                     LTSIAEKEVLMGTVVGMNKKEVVININYKSEGVVPLSEFRYKPDLKLGDQVEVYIEKQ
                     EDKGGQMVISHKTARVHNAWGKVNHALETNEIITGFVKCRTKGGLIVDVFGIEAFLPG
                     SQIDVKPIRDYDQFVGKNMEFKVVKINQEFKNVVVSHKALIEAELEAQKKQIIGGLEK
                     GQVLEGAVKNITSYGVFVDLGGVDGLIHITDLSWGRVSHPEEIVKLDEKINVVILDFD
                     DEKRRIALGLKQLSAHPWDALSADMNVGDKVKGKVMVITDYGSFVEVAAGVEGLLHVS
                     EMSWSQHLRSPKDFLKEGQEIDVQILNIDREERKMSLGMKQLQPDPWADIEFKYPVGS
                     RHTAKVRNFTHFGIFAELEEGVDGLIHISDLSWTKRIKHPSDFCKIGDEVEVQVLEVD
                     KDNRRLSLGHKQVEENPWEVFASVFTPGSTHEGTIVGRNGSSFTVALPYGVEGTVTLK
                     HLKKADGSKAGLEEKLPFVVLEFSSEHRRIALSHTRTFEEGDDVIESAPASAKRGRKG
                     TGDEGGDHSASVKSVNDKVEKSTLGDLSVLSDLKNQMQAGEGGKAKSKAKKEEADDQS
                     EEA"
BASE COUNT          712 a          892 c         1000 g          498 t
ORIGIN      
        1 atgaagatgg cgtccacccg ggcctggtcc aggcttcgtt cccgataggt gtgcgccgca
       61 tcgcggaagc gcccgaagac caacttctcc cgggcgaagg acttgatcac ccggatcccg
      121 ctgaagctct cctgcgccag gctgctgagc acgctctgct gggcctgcac cgccaggccg
      181 cgcttgttca tgaggtcgct cacgtaataa atggcgatgc tcatcagggg gagtggtgcc
      241 agcacccaca gggtgagctt cacattcacc ccgaccatca cccacacgat catcacgctc
      301 agcaccacca tgcccaggat gtacatgatg gccggaccga tgtacatgcg caccttgccc
      361 acgtcctcgc tgatgcggtt catcaggtcc cccgtgctgt ggcccttgta gaagccgcgg
      421 tccagccgct gatagtgggc gaaaatgtcg ttcttcaggt cgtattcgat caggcggctc
      481 accacgatga tcgtctggcg catgaggaac atgaagaagc cttgaagcag cgtgaaagcc
      541 acgaacagca gggcatagaa cgcggcgaac cagcgcacat tggtacgcct gtcctcatca
      601 gtggcagctc cgttgagccg ggcgtccagg tcgataccgg tccaaccgat ccaactgttg
      661 atctcggcag gaacgtccag gtgccgttgg tccagcggtt gatcgcgttg agcgaggctt
      721 gtaccgataa ggtcgaaggc ctcgcgcacc acctgcggct ggaagatctg gaacaagctg
      781 ctgagcgcgg tgaacaagat gcccagcgcc atgcgtcccc aatagcggcg gaaatagggg
      841 ttgagggcga gcagggcacg catggggcgg ggcgaaagta gaggtggccg atgcggccat
      901 gtgcggtcac caggccgttc gcggtacgaa ggcggcggga accttgatcc attgaccctt
      961 ggggagggcg aggagtacgg atgccgatcg gcctgatctt cagcgccgaa gcgcttgtgg
     1021 atcgaacagg aacacgtaca ttcgcgcccc cgaatttttc gggaacgtcc gctgggcagc
     1081 acagcgggcc aacaacaacc ccttccggtc agcgctgccc cgaaacatcg ggaaccggga
     1141 tacaagtcga aagctgaatg tcagcagaac aaaccaaggt caccttcgcc gaaccgcccg
     1201 ccgatttcga ttgggccaca ctggaggatg acaagaagtc catttccgcc agcgatcgcg
     1261 agcggatgga gaaggcgtac gaggagagcc tcaccagcat tgccgagaaa gaggtgctca
     1321 tgggcaccgt tgtcggcatg aacaagaaag aggtcgtcat caacatcaac tacaagtccg
     1381 agggcgtcgt tcccctgagc gaattccgct acaagccgga cctgaagctg ggcgatcagg
     1441 tggaggtgta catcgagaag caggaggaca agggcggtca gatggtgatc agccacaaga
     1501 cggcccgggt acacaatgcc tggggcaagg tgaaccatgc gctcgagacc aacgagatca
     1561 tcaccggctt cgtgaagtgc cgtacgaaag gcggcctgat cgtggacgtg ttcgggatcg
     1621 aggccttcct gcccggcagc cagatcgatg tgaagccgat ccgcgactac gaccagttcg
     1681 tgggcaagaa catggagttc aaggtggtga agatcaacca ggagttcaag aacgtggtgg
     1741 tctcgcacaa ggccctgatc gaagccgaac tcgaggccca gaagaagcag atcatcggtg
     1801 gcctggagaa aggccaggtg ctggagggtg ccgtcaagaa cattaccagt tacggcgtat
     1861 tcgtcgacct gggcggtgtg gacggcctga tccacatcac cgacctgagt tggggccgcg
     1921 taagccaccc ggaagagatc gtgaagctcg acgagaagat caacgtcgtc atcctcgact
     1981 tcgacgacga gaagcgccgc atcgccttgg gcctgaagca actgagcgcg catccgtggg
     2041 acgccctgag cgccgatatg aacgtgggcg acaaggtgaa gggcaaggtg atggtgatca
     2101 ccgattacgg ctcgttcgtg gaagtggccg ccggcgtgga gggcctgctg cacgtgagcg
     2161 aaatgagctg gagccagcat ctgcgcagcc cgaaggactt cctcaaggaa ggccaggaga
     2221 tcgatgtgca gatcctcaac atcgatcgcg aggagcgcaa gatgagcctc ggcatgaagc
     2281 agctgcagcc tgatccctgg gccgacatcg agttcaagta cccggtgggc agccgccaca
     2341 cggccaaggt gcgcaacttc acccacttcg gcatcttcgc cgagctggag gaaggcgtgg
     2401 acgggcttat ccacatcagc gacctgagct ggaccaagcg catcaagcac ccgagcgatt
     2461 tctgcaagat cggcgacgag gtggaggtgc aggtgttgga ggtggacaag gacaaccgcc
     2521 gcctcagcct cggtcacaag caagtggaag agaacccctg ggaagtgttc gccagcgtgt
     2581 tcacgcccgg cagcacgcac gagggcacca tcgtgggacg caacggcagc agcttcaccg
     2641 tggccttgcc gtacggtgtt gaaggcaccg tgacgttgaa gcacctgaag aaagccgacg
     2701 gcagcaaggc cggtctcgaa gagaagctgc cgttcgtcgt gctcgagttc agctccgagc
     2761 atcgccggat cgccctcagc catacgcgca ccttcgaaga gggcgatgat gtgatcgaga
     2821 gcgcacccgc atccgccaag cgtggccgca aaggcactgg cgatgagggc ggcgaccaca
     2881 gcgccagcgt gaagagcgtg aacgacaagg tggagaaatc caccttgggc gacctcagcg
     2941 tgctgagcga cctgaagaac cagatgcagg ccggcgaagg tggcaaagcc aagtccaagg
     3001 ccaagaaaga ggaggccgac gaccagagcg aagaggcctg atccagcaac aagtgacctg
     3061 tgcgaagggg ccgtcctgat cggggcggcc ccttcttttt tt
//
LOCUS       DAOQOC010000225         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16193_length_3101_cov_3.386408, whole genome shotgun sequence.
ACCESSION   DAOQOC010000225 DAOQOC010000000
VERSION     DAOQOC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16193_length_3101_cov_3.386408"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(741..1925)
                     /locus_tag="PL106_04765"
     CDS             complement(741..1925)
                     /locus_tag="PL106_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNA32410.1"
                     /translation="MSQKLISRSADLQRLRNEGYAIEILGGHLVVHDIPYVNAQRKVA
                     QGKLVCPLNLQGDRTGPPSDHVMWFVGEHPCDRDGNIITAIQHSVYNVRLSDEVVTRY
                     SFSNKPADGYPNYYEKVLTYTRVITGQAQAIDPEATAQTFRVMVDGNDSSVFNYIETN
                     SSRAGITELTAKFEGLRVGIIGVGGTGSYVLDQVAKTPVAEIRLIDDDDFLQHNAFRS
                     PGAASKEKLEARPKKVDHQYDTYSKMHKGIKAHAVRITEDNLELLDGLDYVFICIDSN
                     SAKAVILAHLIENGIPFIDCGLGVNTGRGGLIGQVRVTVGTPSFHDHLVNRIGMADSD
                     DDEYATNIQISDLNMLNAALAVIKWKQLCSFYQDQKQAHHVEYVISTGQITREEVPAQ
                     VR"
     gene            complement(1900..2382)
                     /locus_tag="PL106_04770"
     CDS             complement(1900..2382)
                     /locus_tag="PL106_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HNA32411.1"
                     /translation="MKEHDETEKGGTGRQPLKLTINKEIFAWPQQYILGADIRKLGRI
                     GPDELIYLAIKKPWDDELIKNDTRVNLARPEIEHFFSKKTVTLIVNGTPHEWGKPTIC
                     FEDVVRLAYPNAAPSPTRVYTVTYDRGPKENPEGSMVKRDCVCVKDKMTFNVTETDKS
                     "
     gene            2469..>3101
                     /locus_tag="PL106_04775"
     CDS             2469..>3101
                     /locus_tag="PL106_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32412.1"
                     /translation="MIAQQIALEPTETLLEVMKWRNNAEDLVLAKDAFRELCSRLGPD
                     VQRKCKQVCARWKQDPGLAPEIANRTFEKFWNTNSFDPTRWKDKGVELGVKLYLYRTA
                     SNILFDLHEEETSPSPYNGSEHVVREVDELFTSLSPRQLREAKEKIDGALQGLTDKHK
                     VILLTYKKYETDGRKLPRVLLKKLRDELQLTQSTVRFYKKEAFEQFDKTFN"
BASE COUNT          631 a          891 c          855 g          724 t
ORIGIN      
        1 ccgccgatga aaccgaccac cagcgcgccg atcggtcctc cgatcaacag cccaaacccg
       61 gcaccgataa gtgcgcctcc ggcggtgttg gtttcgcgca tggccttcag cttttgccag
      121 gaatagggtg ctcgagtgtt gccgttccaa tcgcgtgcgg tccgggtatt gcaggtaccc
      181 ttcgcgaggt tgcaagggat gcaggccgcg tagaggttgt tgccgtggtt ggttccacca
      241 agcgcacgcg ggcgcgagtg atccacatgc catgagcccc tcgaaccgtg acgtccatag
      301 ttcgagtacg atagctggca atggcagata tgacaatgcc catttgtcct gttgtagatg
      361 cggcagagtt ggtcgttcgt gaaagccatc ttcgtttgtg ttagttgttg ccctcctgtt
      421 ctcgttgtcg gttcttgcgg tagtacttca tctttgcttc ccgatcctct tcacggccct
      481 cctcgacttc ttcgtccgac caacgccgcg cccatcgcac cttgttcctg ataatccagt
      541 agtgcgactt gcatgggaaa ctccagttgc cgatcgaagg gctgagtgtc acctcctttc
      601 cgttgaaccg gagctcccag tccgtcggtg agaacggtgt caccaccctg ctcccgcatc
      661 cgcatgggca aaggtgcact gcggtgccat attccacggt gatgtacagc acgccctcat
      721 ccaagtcctg agggatgaac tcagcgaacc tgtgccggaa cctcctctcg agtgatctgt
      781 ccggtgctga tgacgtactc gacatggtgt gcttgcttct ggtcttgata gaacgaacac
      841 agttgcttcc actttatcac ggcgagcgcg gcgttcagca tgttgagatc tgagatctgg
      901 atgttggtcg cgtactcatc gtcgtccgag tcggccatac cgatgcggtt caccagatgg
      961 tcatggaatg acggcgtccc taccgtgacc cgcacttggc cgatcaaccc acctcggccc
     1021 gtgttaaccc ccaagccaca atcgatgaac gggataccgt tctcgatcag gtgcgcgaga
     1081 atgaccgctt tggcgctgtt ggaatcgatg cagatgaaga catagtccag cccatcgagc
     1141 agttccaggt tgtcctccgt gatccgaact gcatgagcct tgatcccctt gtgcatcttg
     1201 gaataggtgt cgtactggtg gtccaccttc tttgggcgtg cttccaactt ctcttttgat
     1261 gccgcaccag gagaacggaa ggcgttgtgc tgcaagaaat catcatcatc gatcagccgg
     1321 atctccgcaa caggcgtctt cgctacctgg tcaaggacgt acgaccccgt gccacccacg
     1381 ccgatgatcc cgacccgaag tccctcgaac ttggcggtca attccgtgat gcccgcccgg
     1441 ctggagttgg tctcgatgta gttaaacacg gagctgtcgt taccatcgac cattacacgg
     1501 aatgtctgcg ccgtggcctc ggggtcgatc gcctgggcct gtccggtaat gaccctggtg
     1561 taggtcaaga ccttctcgta gtagttcggg tagccgtcgg cgggtttgtt ggagaacgag
     1621 taccgcgtca ccacctcatc agacaagcgc acgttgtaca cgctgtgctg gatggccgtg
     1681 atgatgttcc cgtcccggtc gcacggatgc tcaccaacga accacatcac atggtccgac
     1741 ggtggtccag tccggtcgcc ctgaaggttc agcgggcaca cgagcttccc ctgggccacc
     1801 ttcctctggg cgttcacgta ggggatatcg tggacgacca ggtggccacc gagaatctcg
     1861 atggcgtacc cctcattccg caggcgctga aggtcggcgc tacgacttat cagtttctgt
     1921 gacattgaag gtcattttgt cctttacaca cacgcagtca cgcttcacca tcgatccctc
     1981 ggggttctcc ttggggcctc ggtcgtaggt cacggtgtac accctcgtcg ggctgggggc
     2041 cgcgttcgga taagccagac ggaccacgtc ctcgaagcaa atcgttggct tgccccattc
     2101 atgcggcgtt ccgttcacga tcagggtcac ggtcttcttg gagaagaagt gctcgatctc
     2161 gggtcgtgcg aggtttaccc gcgtatcgtt cttgatcagc tcatcatccc acggcttctt
     2221 gatggcgagg tagatcagct catcggggcc gatgcggccc agcttcctga tatcggcacc
     2281 caagatgtac tgttggggcc atgcgaaaat ctccttgttg atggtcaact tcaggggctg
     2341 gcgaccagtc cctcccttct cggtttcgtc gtgttctttc atctttgctc tggggaaatt
     2401 gttaaggtcc ccagaacaac tacccaaacc gttgccaacg gtttgcagta gtacatatga
     2461 gcggtgccat gatagcccag caaatagctc ttgagcccac cgagaccttg ctcgaggtca
     2521 tgaagtggcg taataacgcc gaggatcttg tgcttgcgaa ggatgcgttc cgtgaacttt
     2581 gcagccgcct tggtccggac gtccagagga agtgcaaaca ggtgtgcgct cgatggaagc
     2641 aagaccccgg tctcgcacca gagatcgcaa acaggacgtt tgagaagttc tggaacacaa
     2701 atagcttcga tccgacgcgg tggaaggaca agggagttga actcggggtg aagctttatt
     2761 tgtatcggac cgccagcaac attctgtttg acctccacga agaagaaacg tccccatcgc
     2821 cttacaatgg aagtgagcac gtagtccgag aggtcgatga attgttcacg tcgctctcac
     2881 ctcgtcaatt gagagaagcc aaagagaaga tcgatggggc attgcaaggc cttaccgaca
     2941 agcacaaggt cattctactg acctacaaga agtacgagac agatggtcgc aagctccctc
     3001 gtgtattgct caaaaagctg cgcgacgaac tgcaactgac ccaaagtacc gtccgctttt
     3061 acaagaaaga ggcctttgag cagtttgaca agacattcaa c
//
LOCUS       DAOQOC010000226         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16218_length_3099_cov_3.594941, whole genome shotgun sequence.
ACCESSION   DAOQOC010000226 DAOQOC010000000
VERSION     DAOQOC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16218_length_3099_cov_3.594941"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2000
                     /locus_tag="PL106_04780"
     CDS             <1..2000
                     /locus_tag="PL106_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA32413.1"
                     /translation="TGMRANGWAFSFSGSRRWANEGYAPGTFYDASAYFVSVEKRINP
                     KHSVYFTGLGAPLKQGREGLAVKEALNLTGDHYYNPNWGWQEGKKRNAVVMNDHKPIL
                     LLGHIFKPDDSTEWATNVMCTFGRDGMTGLNWFDAKNPTPTYYRYLPSYYASSAPAYA
                     ADLTSAWQMDASTQQIDWDALYQANYNNMYTVQDAEGISGNNVSGLRSKYMIQDMRAD
                     PFRIAVNSVWTKAMRHNLHVTAGASWNKQKTHYFKVVDDLLGGDFWVDLNQFAELDVN
                     NPISANNDLEHPNHVVREGDVFGYDYEIHTRLVNAFGQVEKKWDRVEAYAGATISQTR
                     YWRVGNMMNGLFPDISKGPSEKENFLSGGVKAGAVYKLSGRHYLTANATVMSRPATPR
                     NAYLSPRLRPTVIDGLVNEKVLGGEIGYLARMPRLKGRATLYYTRINDQVWNRTFFND
                     DLLANVNYTMTGVDQTHMGLELGLEGKLSPTWTVTAVAAMGRYTYSSRPSATITRDNL
                     DTAVVSGRTVYWKNYRVGGMPQSAYSIGSRYNSPKFWSVGGNVNWFGQIYLDPNPDRR
                     TAEAVEGLITEDPQWNKLLDQPRLNNGVTVDVYANKSWMIQRKYRVALNLSVTNLFDN
                     QDLITGGYEQLRYDRLNPDKFPARYSYLYGRTFFAMLTFSF"
     gene            2007..>3099
                     /locus_tag="PL106_04785"
     CDS             2007..>3099
                     /locus_tag="PL106_04785"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNA32414.1"
                     /translation="MITKHINRLLVALPFAAALFSGCKKEFDSPPRHTPAVGEVITIA
                     ELKAMFTGAPVHFNGPRSVYATVNSDENDGNFYKNVYVQDSTGGLCLRLLNSGGLYIG
                     DSIRIYLPGTVLSPYNGLMQLDSVDVDRNTVKQATLRHIEPLHLTIGQLDPVAHQSML
                     VRLDSVEFVAAEAVGSTWADAVNQQSVNHTLEDCAGNQVLVRTSGYANYASQALPQGK
                     GSFLGVLGVFGSDLQLYARTVGEVALNGPRCPGQELPFFMKNFNDGQPNSNGWSQWSD
                     NNIAWTTNTIGSNDGTPYGQCKNWNGSANIPGESWLISPAVDLTGQNAPTLSFITGCN
                     YNGAALQVLVSTDYTGGAPSAATWTALAPV"
BASE COUNT          686 a         1007 c          905 g          501 t
ORIGIN      
        1 atacgggtat gcgggccaac ggatgggcgt tcagcttcag tggttcgcgg cgctgggcca
       61 atgagggtta cgcacccggc acgttctatg acgcgagcgc gtacttcgtc agcgtggaga
      121 agcgcataaa tcccaaacat tcagtttatt tcacgggatt gggagccccg ctcaaacaag
      181 gtcgggaagg cttggcggtg aaagaggccc tcaacctcac cggcgaccac tactacaacc
      241 ccaattgggg ctggcaggaa ggcaagaagc ggaatgccgt ggtgatgaac gaccacaagc
      301 ccatcctcct gctcggccac atcttcaaac cggacgacag caccgaatgg gccaccaatg
      361 tgatgtgcac gttcggaagg gacggcatga cgggcctcaa ctggttcgac gccaagaacc
      421 ccacgcccac ctactaccgt tacctgccca gctattacgc atcgagcgcc cccgcctatg
      481 ccgccgacct caccagcgcc tggcaaatgg acgccagcac ccaacagatc gattgggacg
      541 cgctgtacca ggccaactac aacaacatgt acaccgtgca ggacgccgag ggcatcagtg
      601 gcaacaatgt ctcgggcctg cgcagcaaat acatgatcca ggacatgcgc gccgacccct
      661 tccggatcgc cgtgaacagc gtgtggacca aggccatgag acacaacctg cacgtgaccg
      721 ccggggccag ctggaacaag cagaagacac actacttcaa agtggtggac gacctgctgg
      781 gcggggattt ctgggtggac ctcaaccagt tcgcggagct cgacgtgaac aaccccattt
      841 cagccaacaa cgacctggag catccgaatc acgtggtacg cgaaggtgat gtgttcggct
      901 acgattacga gatccacacc cggttggtga acgccttcgg acaggtggag aaaaaatggg
      961 accgggtgga ggcttatgcc ggcgccacca tttcccagac ccgttactgg cgtgtgggca
     1021 acatgatgaa cggcctgttc cccgacattt ccaaaggccc cagcgagaag gagaacttcc
     1081 tgagcggcgg tgtgaaagcg ggtgcggtct acaaactgag cggtcgccac tacctcacgg
     1141 cgaacgcgac cgtcatgtcc cggccagcca ccccgcgcaa cgcctacctc agcccccggc
     1201 tgcgccccac cgtgatcgac gggctggtga acgaaaaggt gctcggtggg gagatcggtt
     1261 acctggcacg catgccccga ctgaagggcc gtgcgacgct ttattatacc cggatcaatg
     1321 accaggtctg gaaccggacg ttcttcaacg acgacctgct ggccaacgtg aactacacca
     1381 tgaccggggt tgaccagacg cacatgggcc tggaactcgg gctggaaggc aagctcagcc
     1441 ccacatggac cgtcaccgcc gtggccgcca tgggccggta cacgtacagc agtaggccct
     1501 cggccacgat cacccgggac aacctggaca ccgccgtggt ctccgggcgc accgtttact
     1561 ggaagaacta ccgcgtgggc ggcatgcccc agagcgccta cagcatcgga tcccggtaca
     1621 attccccgaa attctggagc gtcggtggca atgtcaactg gttcggccag atctatctgg
     1681 accccaatcc ggaccgccgc accgccgagg ccgtggaagg cctcatcacc gaggatcccc
     1741 agtggaacaa actgctcgat cagcccaggt tgaacaacgg cgtcaccgtg gacgtgtatg
     1801 ccaacaagag ctggatgatc cagcgcaagt accgcgtggc gctgaacctg agcgtgacca
     1861 acctgttcga taaccaggac ctgatcaccg gcgggtacga gcaactccgc tacgaccggc
     1921 tgaaccccga caagttcccc gcaaggtaca gctacctgta cggccgcacc tttttcgcca
     1981 tgctcacctt cagcttctga ccccgcatga tcaccaagca catcaaccgc ctgctcgtcg
     2041 ccctgccgtt cgccgcggcc ctgttcagcg gctgcaaaaa ggaattcgat tcaccgccca
     2101 ggcacacgcc cgccgtgggc gaggtcatca ccatcgctga actgaaagcg atgtttaccg
     2161 gtgctccggt acacttcaac ggccccagga gcgtatacgc cacggtgaac agcgatgaga
     2221 atgacggcaa cttctacaag aacgtgtacg tgcaggactc cacgggcggg ctctgcctgc
     2281 gcctgctcaa cagcggtggg ctgtacatcg gcgacagcat ccgcatctac ctgccgggca
     2341 ccgtgctctc cccgtacaat gggctgatgc aactggacag cgtggacgtg gaccgcaaca
     2401 cggtgaaaca ggccacgttg cgccacatcg aaccgctcca cctgaccatc ggccagttgg
     2461 acccggtggc ccaccagagc atgctcgtcc ggttggacag cgtggaattc gtggccgctg
     2521 aggccgtggg cagcacgtgg gccgatgccg tgaaccagca aagcgtgaac cacacgctgg
     2581 aggattgcgc gggcaaccag gtcctggtgc gcaccagcgg ttacgcgaac tacgcgagcc
     2641 aggcgcttcc ccaagggaag ggctccttcc tcggtgtgct cggcgtgttc ggcagcgacc
     2701 tgcaactgta cgcacgtacc gtgggagaag tggcgctgaa cggcccccga tgcccgggcc
     2761 aggagctccc cttcttcatg aagaatttca acgatggcca gcccaattcg aatggctggt
     2821 cgcagtggtc cgacaacaac atcgcatgga ccacgaacac catcggatcg aacgatggta
     2881 cgccctacgg ccaatgcaag aactggaacg gatcggccaa catccccggg gaatcgtggc
     2941 tcatctcccc tgcggtggac ctgaccgggc agaatgcgcc gacgctcagc ttcatcaccg
     3001 ggtgcaacta caacggtgcc gcattgcagg tgctggtgag cacggactat accggcgggg
     3061 caccgtccgc cgccacatgg accgccctgg cccccgtac
//
LOCUS       DAOQOC010000227         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16245_length_3095_cov_3.025658, whole genome shotgun sequence.
ACCESSION   DAOQOC010000227 DAOQOC010000000
VERSION     DAOQOC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16245_length_3095_cov_3.025658"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PL106_04790"
     CDS             <1..1179
                     /locus_tag="PL106_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNA32415.1"
                     /translation="SREALEDCHYKGGKDLIALHYAHRPDSKLYQGMNMVVGGSSTIE
                     NLRADAYSGDLSAIIIRNLPVWVEIRRTPVIETALMEHWEEKIERMARETMREDVRSI
                     AGVPSWTLVILRRILELTGKTNIHEVWPNLELFMHGGVSFGPYREQFARLFPAAGMNY
                     LESYNASEGFFGITDERGSGDLLLMLDYGIFFEFIPVEELHREQPPTKLLHEVVPDHQ
                     YAIVISTNAGLWRYMPGDTIRFSSVEPYRFRVTGRTRSFINAFGEELIVENAERGIEA
                     ACAATGAVVSEYTAGPVYMDLDARGGHEWVIEFERPPADINAFVEALDRRMRELNSDY
                     DAKRRGDMALVAPKVHTVRQGTFFQWMKERGKLGGQNKVPRLCNDRQWLDQLLHTMDA
                     "
     gene            1176..1982
                     /locus_tag="PL106_04795"
     CDS             1176..1982
                     /locus_tag="PL106_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32416.1"
                     /translation="MKPLILPLLAVMGVGALAQDPAGAVVIKGNFDRFTSDELGNVYT
                     LQGDEIALWNAKGVRLLRNSLKTFGEITTIDAFSSLKPMIFSSPQGMLVELDNTLAIQ
                     ADPIDLNRNGFPQVTLACMSVQNMFWFFDERLMRLTRVDHQLRTVASTGRLDQMLGIS
                     PHPILMTEADSWLYVLDPELGVFVFDIFGGYAKTLPIVGTASIEVRDGRILYLKDGVL
                     HSYDLKGHTIGMPVTWLSTYREKGLRDARIERGLLYALYPDRIVVAPLGP"
     gene            2155..2820
                     /locus_tag="PL106_04800"
     CDS             2155..2820
                     /locus_tag="PL106_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNA32417.1"
                     /translation="MHIAIAGNIGAGKTTLTQLLAKHYKWDMLQEAVDNNPYLFDFYK
                     DMQRWSFNLQIFFLNSRFEQLLEIRRSGRNVIQDRTIYEDANIFAPNLHAMGLMTTRD
                     FENYTRLFNNMDASIVPPDLMIYLRARVENLVDQIAERGRDYETSISIDYLKRLNERY
                     EAWIDTYDKGKLLIVDIDDNRFHKEPEDLGTIIHAIDAEIHGLFPSENGKGAKVGVVR
                     KKR"
     gene            complement(2836..>3095)
                     /locus_tag="PL106_04805"
     CDS             complement(2836..>3095)
                     /locus_tag="PL106_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNA32418.1"
                     /translation="GKAKVKIEPGTQSNHTLRLKGKGLPSVSHHGTGDLFVHLMVWTP
                     SNLSKEERGALEKLRASPGFQPRPTAKDKGFFERVKEMFGN"
BASE COUNT          640 a          984 c          947 g          524 t
ORIGIN      
        1 agcagggagg cgctggagga ctgccattac aagggcggca aggacctgat cgccctgcac
       61 tacgcacacc ggccggacag caaattgtac cagggcatga acatggtggt gggcggcagc
      121 agcaccatcg agaacctccg tgccgacgcg tacagcggcg acctcagcgc catcatcatc
      181 cgcaacctgc cggtctgggt ggagatccgc cgaacgccgg tgatcgagac cgcgttgatg
      241 gagcactggg aggagaagat cgaacgcatg gcacgcgaga ccatgcgcga ggacgttcgc
      301 agcatcgcgg gcgtgccatc gtggactctg gtgatcctgc ggcgcatcct cgaactcacg
      361 ggcaagacga acatccacga agtgtggccc aacctggaac tcttcatgca cggcggcgtc
      421 agtttcggtc cgtaccgcga acagttcgca cggctctttc cggctgcggg catgaattac
      481 ctggagagct acaacgccag cgagggattt ttcggcatca ccgacgaacg cggatcgggc
      541 gacttgctgc tgatgctcga ctacggcatc tttttcgagt tcatcccggt ggaagagctg
      601 catcgggaac aaccgcccac caagctgctg cacgaggtgg taccggacca ccagtacgcc
      661 atcgtcatca gcaccaacgc cgggctgtgg cgctacatgc cgggcgacac catccggttc
      721 agcagcgtgg aaccctaccg gttccgcgtt acgggccgca cacgcagctt cataaacgcc
      781 ttcggcgagg aactcatcgt ggagaacgcc gagcggggca tcgaagccgc gtgcgcggcc
      841 acgggggcgg tggtgagcga gtacacggcc gggccggtgt acatggacct cgacgcccgc
      901 ggcgggcacg aatgggtgat cgagttcgaa cggccgcccg ctgacatcaa cgctttcgtg
      961 gaggccctgg accgacgcat gcgcgagctg aacagcgact acgacgccaa acgccggggc
     1021 gacatggccc tggtggcgcc caaagtgcat accgtgcgtc agggcacctt cttccaatgg
     1081 atgaaggaac gcggcaaatt gggcggccag aacaaggtgc cgcggctgtg caacgaccgc
     1141 caatggttgg accagttgtt gcacacgatg gacgcatgaa gcccctcatt cttcccctgc
     1201 tcgcggtcat gggcgtgggc gcgctcgcac aggaccccgc tggcgccgtg gtcatcaagg
     1261 gcaacttcga ccggttcacc agcgatgaac tgggcaatgt gtacaccctg caaggcgacg
     1321 agatcgcgct gtggaacgcg aaaggcgtgc gactcctgcg caacagcctg aagaccttcg
     1381 gggagatcac caccatcgac gcgttcagct ccctgaagcc gatgatattt tccagcccgc
     1441 agggcatgct ggtcgaactg gacaacaccc tggccataca ggccgacccc atcgacctga
     1501 accggaacgg cttcccgcag gtgaccctgg cctgcatgag cgtgcagaac atgttctggt
     1561 tcttcgacga acggctgatg cgcctcaccc gcgtggacca ccaattgcgc accgtggcct
     1621 ccaccggccg actcgaccag atgctcggta tcagcccaca tccgatcctc atgaccgagg
     1681 cggacagctg gctctatgtc ctggatcccg aactgggcgt gttcgtcttc gacatcttcg
     1741 gcggctatgc gaagacactc cccatcgtgg gtacggcatc cattgaggtg cgcgacggac
     1801 ggatcctcta cttgaaggac ggcgtgctgc acagttatga cctgaagggc cacaccatcg
     1861 ggatgccggt cacatggctc tccacgtacc gggagaaggg tttgcgcgat gcgcggatcg
     1921 aacgcggctt gttgtacgcg ctctatcccg atcgcatcgt tgtggcccct ctcgggccat
     1981 gagcgccgca ccgggtgcgt ggcgcagcag gatcacatcc gttgaagcaa cgcatctgct
     2041 gatcccgcaa tgacaggtca cgctgcgcga gaggtccaac gacttatcca cagatcgaga
     2101 atgcggcgcg cacgcgggta aattcgcggc ccttcacgtc tacggaacag gaacatgcac
     2161 atcgcgatcg cgggtaacat cggcgcgggc aaaaccacgc tcacgcaact gctggccaaa
     2221 cactacaagt gggacatgct gcaggaggcg gtggacaaca atccctacct cttcgacttc
     2281 tacaaggaca tgcagcgctg gagcttcaac ctccagatct tcttcctcaa cagccgcttc
     2341 gagcagctgt tggagatccg ccgaagcggg cgcaacgtga tccaggaccg caccatctac
     2401 gaggacgcca acatcttcgc cccgaacctg cacgccatgg ggctgatgac cacgcgcgac
     2461 ttcgagaact acacgcgcct gttcaacaac atggacgcct cgatcgtgcc gcccgacctg
     2521 atgatctacc tgcgcgcgcg ggtggagaac ctggtggacc agatcgctga gcgcggccgc
     2581 gactacgaga cgagtatcag catcgactac ctcaagcgtc tgaacgagcg ttacgaagcc
     2641 tggatcgata cctacgacaa gggcaaattg ctcatcgtgg acatcgacga caaccggttc
     2701 cacaaggaac ccgaggacct gggcaccatc atccacgcca tcgacgcgga gatccacggc
     2761 ctgttcccca gcgagaacgg caaaggtgcc aaggtgggcg tggtacgcaa gaagcggtag
     2821 accgcgtacg cgggatcaat tcccgaacat ctccttcacg cgctcgaaga agcccttgtc
     2881 cttggcggtg ggccggggtt ggaacccggg gctggcgcgc aacttctcca gtgcgccgcg
     2941 ctcctccttg ctgaggttgc tgggcgtcca caccatcagg tgtacgaaaa ggtcgccggt
     3001 gccatggtgg ctcacactgg gcaggccctt gcccttgagg cgcagcgtgt ggttgctctg
     3061 ggtgccgggc tcgatcttca ccttggcctt gccac
//
LOCUS       DAOQOC010000228         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16251_length_3094_cov_3.775255, whole genome shotgun sequence.
ACCESSION   DAOQOC010000228 DAOQOC010000000
VERSION     DAOQOC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16251_length_3094_cov_3.775255"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..457
                     /locus_tag="PL106_04810"
     CDS             80..457
                     /locus_tag="PL106_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA32419.1"
                     /translation="MEAVLIDTSVLIGRFRRESRAMAAMERIVGAQFCLCDAIIAEVL
                     AGTRNKAEFALTKRELFEQFHLLPLTMEVSQRFRSILDGLELGRDIHFADQLIAATAM
                     AHDVPLLTLNKKHFTPIKGLKLA"
     gene            complement(454..1146)
                     /locus_tag="PL106_04815"
     CDS             complement(454..1146)
                     /locus_tag="PL106_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32420.1"
                     /translation="MKHHTTLLLLLASATTNAQNAYSFERTQQPYAELMDATFCDFNS
                     DGDDPLPELNGETFVLYGQAWTGTSSYPITIGGHGFLRIENASALVILDGFFTNIEAV
                     DSMSNVSYAITGEPGARVLTAQWHNIRLVNGPDDSYLNYQIRLYQATGVVEVHMGPNS
                     GSAIEYSDSSGPNCGVFHSPQSFSGCLGKLWVEQDANSPTLDSLPNYDFDALHNLPLP
                     NTLYRFTPPVHG"
     gene            1370..2908
                     /locus_tag="PL106_04820"
     CDS             1370..2908
                     /locus_tag="PL106_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32421.1"
                     /translation="MRTTTLIPLVTLAMHLHGQAGLLDNTFSGDGKATFAGTGDTYFT
                     AVVIQPNGKVVAVGSTGTSNVDFLVVRFNSNGTLDTSFDGDGYATKGLPGEDRASCVA
                     LQGDGKILVGGYYTVGANRYMAIVRLNADGTPDTGFGLNGQNFIAFDQESPTYAEGLG
                     IAVQADGRIVVCGSTGDATDQDADLALARFDADGSIDSGFGSEGKVTDDVGTVNNSGN
                     VVAVDNNGRLVIAGNTGSGNQSCVVHRFLTDGVPDAAFNGGHFSFDIVSEADHALHGM
                     VLQPNGSVLVAGSCGGQFGATMLNANGYWDGSFGAEGKVQLVSPENGAQGYALCKQAD
                     GKFLIAGTYEHSGAPSDLCLARMNGDGSIDASFGTNGWAYYDIETLVDDGRGMALQSD
                     GKVVVVGTDNSVPKKGSVIRVLNDVGIGMSEARTPALSVSAFPDPATDHISLRYTLRA
                     QDRVSGRILDATGRPVRAVFAQAQRIPGTHTERVDLDGLACGGYLLELRTDQGVVRTP
                     FIKQ"
BASE COUNT          610 a          871 c          988 g          625 t
ORIGIN      
        1 cgcaccgcag agaattctag cagaattccg cgatatggaa ggacaatccg gtcacggcgg
       61 atgagttgcg tgaaagggca tggaagcggt cctgatcgat acgagcgtcc tgatcggacg
      121 attccggcgg gaatcccggg cgatggcggc gatggagcgc atcgtggggg cccagttctg
      181 cctgtgtgac gcgatcatcg cggaagtgct tgccggaacc cggaacaaag cggaattcgc
      241 actcaccaaa cgcgaactat tcgagcagtt ccatctgctc ccactgacga tggaagtgtc
      301 ccagcgcttc cggtccattc ttgacggact ggaacttgga cgggatatcc acttcgccga
      361 tcaactgatc gcagccacgg ccatggcgca cgatgtgcct ctgctcactc tgaacaagaa
      421 gcacttcacc ccgatcaagg ggctgaagct cgcttagccg tgcacgggcg gcgtgaagcg
      481 atagagcgtg ttcggcaggg gcaggttgtg cagcgcatcg aagtcgtagt tcggcagcga
      541 atcgagcgtg ggggagttgg cgtcctgttc cacccagagt ttgccgaggc agccgctgaa
      601 gctctgtggg gagtggaaca ccccgcagtt cgggcctgag ctgtccgaat actcgatagc
      661 gctgccggag ttcgggccca tgtgcacttc cacgacgccg gtggcctgat acagccggat
      721 ctggtagttc aagtagctgt catccggccc gttgaccagt cggatgttgt gccattgtgc
      781 ggtgagcacg cgcgcacccg gctcaccggt gatggcgtag ctcacgttgc tcatgctgtc
      841 gaccgcctcg atgttggtga agaaaccgtc gaggatcacc aacgcggacg cgttctcgat
      901 gcgaaggaac ccgtggccgc cgatggtgat gggataggag ctggtgcccg tccaggcctg
      961 gccatacagc acgaaggttt caccattgag ttcgggcagc gggtcgtcgc catcgctgtt
     1021 gaagtcgcag aaggtggcat ccatgagttc tgcgtatggt tgttgcgtcc gctcgaagga
     1081 gtaggcattc tgcgcgttgg tcgtggcgct ggccagcagg aggagaaggg tcgtgtgatg
     1141 cttcatatgt cggcgttttg gagcggacgg tcgaagcgcg tgtccaggtg cccgcgttgc
     1201 ctcttggaac aggccattcg aaccggcctg gctacatctc caggcgaacc gtgcatcctg
     1261 gccgtaccgt tcgtgcgggg acgaggcgtg ggctgcgatc ccctcgatcg ggctggcgat
     1321 cccctcagtg gcgcatcgct atctagaatg agcttcctct cttcggtcca tgagaacaac
     1381 tacccttatc ccccttgtaa cgctcgccat gcaccttcac ggacaggcgg gcctgttgga
     1441 caatactttc agcggagatg gcaaagcgac attcgccgga acgggtgaca cctatttcac
     1501 ggccgtcgtt atccaaccca acggaaaggt cgtggcggtc ggctccacgg gcacaagcaa
     1561 cgtggacttt ctcgtggtcc gcttcaactc caatggcaca ctggatacat cctttgacgg
     1621 cgatggatat gccacaaagg ggctaccggg ggaggaccgg gcatcgtgtg tcgccctgca
     1681 gggagatgga aagatcctgg tggggggcta ctacaccgtc ggggccaacc gctacatggc
     1741 catcgtccgg ctaaatgccg acggtacgcc agataccggc ttcggtttga acggacagaa
     1801 cttcatcgcc ttcgaccagg agtccccgac ctatgcagag gggcttggca tcgcggttca
     1861 agcggatggt cgcatcgtgg tttgtggttc gaccggcgat gccaccgatc aggacgccga
     1921 cctggccttg gcacggttcg acgctgatgg ttctatcgac tcaggctttg gatccgaagg
     1981 caaggtcacg gacgacgttg gcaccgtgaa caattccggg aatgttgtgg ctgtggataa
     2041 caacggccga ttggtgatcg cgggcaatac tggttctggg aaccaatcct gcgttgttca
     2101 ccgctttcta acggatgggg tgccggacgc ggcgttcaat ggaggacatt tcagtttcga
     2161 cattgtttcg gaggctgacc acgctctgca tggaatggtt ctccagccca acggtagtgt
     2221 gctcgtagcg ggtagctgtg gaggtcaatt cggcgccacg atgctgaacg cgaacggtta
     2281 ttgggacgga tcttttggag cggagggtaa agtacaattg gtgtctccgg aaaatggtgc
     2341 tcaaggctat gcgctttgta agcaggccga cggaaaattc ctgatagccg gcacgtatga
     2401 acacagcgga gcaccatcgg atctttgctt ggcccgcatg aacggtgacg gaagcatcga
     2461 cgcttctttc ggcacgaacg gttgggccta ctacgacatc gaaaccctgg tggacgatgg
     2521 ccgaggcatg gccttgcaga gtgatgggaa ggtcgtggtg gtgggaacgg acaactcagt
     2581 accgaaaaag ggttcggtga tccgtgtact gaacgacgtc ggcatcggca tgtccgaagc
     2641 cagaactcca gctctaagcg tgtccgcctt cccggatccc gcgaccgatc atatcagcct
     2701 caggtacaca ttgcgtgcac aagaccgcgt gagcggccgg atcctggacg caaccggccg
     2761 tcccgtgcgc gcggtgttcg cacaggccca gcgcatcccc ggcacgcata ccgaacgggt
     2821 cgacctggat gggctcgcgt gcggcggcta cctccttgaa cttcgcacgg accaaggagt
     2881 ggtgcgcaca cccttcatca aacagtgatt ttcgcccggc ctgcttgagc aacgggccgg
     2941 gcataccgcg caggcgggga aatgaccaac ttgctccatg cgcggtagca ttccggcggt
     3001 actgctctcc ctgtcgcttt gcaccgcgca tgggcagttc ttcaccaact acggagcgca
     3061 agagggcctg accaatcagg acctgggctc cgtg
//
LOCUS       DAOQOC010000229         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16267_length_3093_cov_3.233706, whole genome shotgun sequence.
ACCESSION   DAOQOC010000229 DAOQOC010000000
VERSION     DAOQOC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16267_length_3093_cov_3.233706"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PL106_04825"
     CDS             complement(<1..1613)
                     /locus_tag="PL106_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32422.1"
                     /translation="MKDQNGFDELARQKLAERDFTFDPSHWTAMEGLLARPGRKRGAW
                     WPWLAAGVLLVGGATIWITAREHDPAASVTNPGGGPRAIAGEVKGVNGSAAITEMENA
                     SYGNDHPGTMNADPAVPVTGETRATAGTMRGETSGEPRMRTEATRTRKEHGTGTSDRG
                     TVTMQVNKIPVSSISVRQGEDPHAANVRTGEATNDVSSTMTGPGAAAVAGDGASDERS
                     NLATGAVDPVRSDASAAPAGGGTHGEEQPDSVLQHQPPSAGTGPVVVAGSGDSASTGA
                     TAAPAPVDPVAAALVPPVWLAITAPYELTLLGGAFSTTGRYTGVDAESWPARTERQWT
                     TGFGLEAVRNFGRHFAVGTGVHFSSYKERLITDEVSRTEQQLSTSYFWVAHDTMVTIV
                     VGDTAINGATYHMTQLVPMTINELGVDTDTAYQTMVIRQRRSALNTVSYVELPLLLDA
                     HTARGRWVFGLRGGPSVGLLTGRSGSLPDNSGEGYHTLNEVAFRELTVGWTARAYVRY
                     RLSAGWSVGVEPTMRGQLFNSLRNADLDRR"
     gene            complement(1610..2173)
                     /locus_tag="PL106_04830"
     CDS             complement(1610..2173)
                     /locus_tag="PL106_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA32423.1"
                     /translation="MINDHIIAGCVRQEPKAQYELYRALYPMMMAICTRYERDRQEAA
                     ATMNQGFLKILDNIGTRRKEVPVEPWARRIMINTVIDAHRRNKERKAMEGADIDDHAE
                     GTSGINDYLKDMEAEAFAELLHRVPAMSRTVFNLYAIDGFSHAEIAGMLGLSEGTTKW
                     HVSHARTVLRNAIAAEAMARTEKTVSR"
     gene            complement(2238..>3093)
                     /locus_tag="PL106_04835"
     CDS             complement(2238..>3093)
                     /locus_tag="PL106_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32424.1"
                     /translation="LGVMYGNAVAGTPCTTFLGLSGEWNADCVCIPDSMNTGDDCLGI
                     PNGPNVPGTPCIVPGTTLDGLWNTDCVCVPDDPMPCQADFWVMQAMGQDSLPVPYELW
                     IWNLSSGGSGNFQYFWDFGDGTNSTEAFPTHTYSGNGPYTLCLTIADNQNCTSTHCDS
                     ISINGDGIYEGMMVHDGIRQEGFTINVQNGAPSAVPDVTITNNLALWPNPVADELNIA
                     IAGGMKGMVTVTVTDMDGRTVSAEGMTLAGGRGQLRIATRNLPSGMYMLRISNGSTNL
                     SQRFVKTN"
BASE COUNT          511 a         1033 c          899 g          650 t
ORIGIN      
        1 cgtcgatcaa gatcagcgtt gcgcaaactg ttgaacaact ggccacgcat cgtgggctcc
       61 acgcccactg accagcctgc ggagaggcga taccgtacgt aggcccgggc cgtccaaccg
      121 acggtcagtt cccggaaggc cacttcgttc aaggtatggt atccctcgcc gctgttgtcg
      181 ggcaggcttc cgctacgacc ggtgagcagg ccgaccgatg ggccaccgcg cagcccgaaa
      241 acccatcgtc cccgcgcggt gtgcgcgtcc agcagcaagg gcagttccac ataactcacc
      301 gtgttcagtg ccgagcgacg ctggcggatc accattgtct ggtacgccgt gtccgtatcc
      361 acccccaact cattgatggt catgggcacc aattgggtca tgtggtaggt ggcgccgttg
      421 atggcggtat cgcccaccac gatcgtcacc atggtgtcgt gggccaccca gaagtacgac
      481 gtactcagct gttgttcggt ccgactcacc tcgtcggtga tcaaccgctc cttgtagctg
      541 ctgaaatgga ccccggtacc caccgcgaag tgcctgccga aattgcgcac ggcttccagg
      601 ccgaagcccg tggtccactg ccgttccgtt cgtgccggcc aggactccgc atcaacgcct
      661 gtgtaccgac ctgttgtact gaaggcgccg ccgagcaacg tgagttcgta gggtgcggtg
      721 atcgccaacc acaccggggg gacaagtgct gctgcaaccg ggtccactgg agcaggcgct
      781 gctgtcgcac ctgtcgatgc gctatcgccc gatccagcca caacgaccgg tccggtcccg
      841 gcagaaggag gttgatgctg caggacgcta tcgggctgct cttccccatg cgttccgcca
      901 cctgcaggcg ctgccgatgc atcactccgc acgggatcga ccgcacccgt ggccaggttg
      961 ctcctttcgt ccgacgcccc atccccagct accgcagccg cacctggacc ggtcatggtc
     1021 gacgacacat cgttcgttgc ctccccggtc ctgacgttcg ccgcatgtgg atcctcaccc
     1081 tgccggacgc taatagaaga taccggtatc ttattgacct gcatggtcac cgtgccccga
     1141 tcggatgttc ctgtcccatg ttccttgcgc gtacgagtgg cttccgttct catccttggc
     1201 tctccggacg tctcaccccg catggtaccc gccgtcgccc gtgtttctcc ggtcacggga
     1261 accgcagggt cggcgttcat tgttcctgga tgatcgttcc cgtatgaagc attctccatc
     1321 tccgtgatcg ccgcactccc gttcactccc ttcacttccc ctgctatcgc acgcggtccg
     1381 ccccccgggt tcgtgacgct ggccgccgga tcatgctccc gggccgtgat ccagatggtc
     1441 gcgccgccca ccagcagcac gcctgcagcc aaccatggcc accacgctcc acgcttccgt
     1501 ccggggcggg ccagcaggcc ttccatggcg gtccagtgcg acgggtcgaa cgtgaagtcg
     1561 cgttcggcca gtttctgccg cgccaattcg tcgaatccgt tctggtcttt catcgggata
     1621 cggtcttttc ggtacgcgcc atcgcctctg cggcgatggc gttgcgcaac acggtgcgcg
     1681 cgtgcgatac gtgccatttc gtggtgcctt cgctcaaccc cagcatcccg gcgatctcgg
     1741 cgtggctgaa tccgtcgatg gcgtagaggt tgaacacggt gcgggacatc gcgggcaccc
     1801 ggtgaaggag ctcggcgaat gcttcggctt ccatgtcctt caggtagtca ttgatcccac
     1861 tggtaccttc cgcgtggtcg tcgatgtccg caccctccat cgccttgcgc tccttgttcc
     1921 gccggtgtgc atcgatcacc gtgttgatca tgatccgcct ggcccagggt tcgacgggca
     1981 cctccttgcg ccgtgttccg atgttgtcca ggatcttcag gaagccctgg ttcatggtgg
     2041 cggccgcctc ctgccggtcg cgctcatacc gcgtgcagat cgccatcatc atggggtaca
     2101 aggcacggta caattcgtac tgcgccttgg gttcctggcg gacgcaaccg gctatgatgt
     2161 ggtcgttgat catgggacct cgtgcccgag gggctccccc atgatcggga cgcccctcgg
     2221 gccggttcag gtcgggatca gttcgtcttc acgaatcgct ggctcaggtt cgtggatccg
     2281 ttgctgatgc gcaacatgta catgccagag ggcaggttgc gggtcgcgat gcgcagctga
     2341 ccgcgaccac ccgccaaggt catcccttct gcactcactg tgcggccatc catgtcggtg
     2401 accgtcaccg tcaccatccc cttcatgccg cccgcgatgg cgatgttcag ctcgtccgcc
     2461 acggggttcg gccaaagtgc caggttgttg gtgatggtca cgtccggaac agcgctcggc
     2521 gcaccgttct gcacattgat cgtaaagcct tcctgacgga tcccgtcatg caccatcatg
     2581 ccctcgtaaa tgccatcacc gttgatggag atgctgtcgc agtgggtgct tgtgcaattc
     2641 tggttgtccg cgatcgtgag gcagagcgtg tacgggccat tgccgctgta tgtatgcgtg
     2701 gggaatgctt cggtggaatt ggtaccgtca ccgaaatccc agaagtactg gaagttgcca
     2761 ctgcctccgc tgctcaggtt ccagatccag agttcgtacg gtacgggcag actatcctga
     2821 cccatggcct gcatcaccca gaaatcggcc tggcagggca tcggatcatc cgggacgcac
     2881 acacaatccg tgttccaaag gccatcaagc gtcgtgcccg gaacgatgca tggcgttcct
     2941 ggcacgttcg ggccattcgg gatcccgagg cagtcatcgc cggtattcat cgaatccggg
     3001 atgcacacgc aatccgcgtt ccactcgccg gacagaccca ggaaggtggt acatggcgta
     3061 ccggccaccg cattaccgta catcacgccc aag
//
LOCUS       DAOQOC010000230         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16313_length_3089_cov_3.156889, whole genome shotgun sequence.
ACCESSION   DAOQOC010000230 DAOQOC010000000
VERSION     DAOQOC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16313_length_3089_cov_3.156889"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PL106_04840"
     CDS             <1..957
                     /locus_tag="PL106_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNA32425.1"
                     /translation="IALFYRPLMRLWTAPNGWLHYAWSLVSVSIAAQLMTAPLALWSF
                     GAFPVWFLPANLVVVGLVALGVYGGAALLALQWIPVLGGVCAWVLVVLLKLIGWTTDL
                     FAWLPGAYPAVRINGWQCAVLYVIILLLAAVFFERWRWARSGLLAMIVLLLTTWAWNA
                     HVRNKQHRFVVYDDRDQLICAMESGRLLTVFTDSLDQWSERKVDTHRRAIGARGMNTD
                     PVPEQLIMDRLVIRWLRGGDRAVPADPSADLLVITDDARYDAERWLSPVPPTRGLVLA
                     PVVSARRRSFWRRWCMEHHVPVHDVKLHGAFILPIHALAREP"
     gene            1021..1386
                     /locus_tag="PL106_04845"
     CDS             1021..1386
                     /locus_tag="PL106_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNA32426.1"
                     /translation="MARRQRVRWHELVEPQAGSLPEGRPVRVMVGKVAVCLVRYEGRL
                     FALEDVCPHQGRSFTGGSCERGRIICPWHLKSFDLITGQGLLASEGNARVFPVEEKNG
                     QVRIGMPRRSFSLFGLRLG"
     gene            complement(1674..2540)
                     /locus_tag="PL106_04850"
     CDS             complement(1674..2540)
                     /locus_tag="PL106_04850"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNA32427.1"
                     /translation="MMRATLILNPRSGKGRAPDVLELARALSRQLGVDLSERMIEGPG
                     HGSELAREAVTAGHDRVIAAGGDGTLNAVANGLVGSPVALGIVAMGSGNGYARSQKLP
                     LNPKEALHRAFTGTARPMDVCYLNDHLFLGTAGIGFDARVAWEFDRSRSRGFWNYLRI
                     TLKEILGAQPLRVVVNANNESTEHRLLMLVFANTREFGNGVAISPGSAPDDGLAELRC
                     VSKPSFLPLIKALFDVYTGRADSSPYIRTIVAREAHIHQDGNLAHLDGEPLVIGNEVR
                     FRLEPARLLVIT"
     gene            complement(2537..>3089)
                     /locus_tag="PL106_04855"
     CDS             complement(2537..>3089)
                     /locus_tag="PL106_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNA32428.1"
                     /translation="IHPTLLYADNALREFFRTAEKMSWFKNTLFVITADHTADLERNG
                     EQSGSAYDFWIPLVYYMPGVIVPRADVRTTQQIDILPTVLDLIGHNEPFFAFGSSTLR
                     LERSPAAVSEGSATWLIVTPRAQLRSDGERILWHDGIGSSWHPEQRTIPNAFDLITAH
                     ALLQAAIQQYNNHLLARDMVVTE"
BASE COUNT          510 a          924 c         1047 g          608 t
ORIGIN      
        1 atcgcgctgt tctatcggcc attgatgcgg ctctggacag caccgaacgg gtggctccat
       61 tacgcctggt cgctcgtttc ggtcagcata gcggcccaac tgatgaccgc accgctcgcg
      121 ctatggtcct tcggggcctt cccggtatgg ttcctgccgg ccaacctggt ggtggtaggg
      181 cttgtggcgc tgggcgtcta tggtggggcg gcgctgctcg ccctgcaatg gatcccggtt
      241 cttggtgggg tctgtgcctg ggtgctggtg gtgctgctga agctgatcgg atggaccacg
      301 gatctcttcg cctggctgcc cggggcgtat ccggcggtac gcatcaacgg ctggcagtgc
      361 gcggtgctgt acgtgatcat cctgctcctg gccgcggtgt ttttcgaacg gtggcggtgg
      421 gcccgttccg gattgttggc catgatcgtt ctgctgttga caacgtgggc ctggaacgcg
      481 cacgtgcgga acaagcagca tcgattcgtg gtgtatgacg atcgcgacca gctcatttgt
      541 gcgatggaga gcgggcggtt gctcaccgtc ttcaccgaca gcctggacca atggtcggag
      601 cgcaaggtgg acacgcaccg ccgggcgatc ggagcccgtg gcatgaacac ggacccggta
      661 ccggaacagc tgatcatgga ccgcctggtc attcgatggc ttcgcggtgg cgaccgggca
      721 gttcctgcgg atccctcggc ggacctcctg gtcatcaccg atgatgcacg ttatgatgcg
      781 gaacgttggc tcagcccggt gccgccaacc cgaggcctcg tactcgcgcc tgtggtgagt
      841 gccaggcgac gctcgttctg gcgccggtgg tgcatggagc atcatgtccc tgtgcatgac
      901 gtgaaactgc acggtgcgtt catattgccg atccacgcgt tggcccggga gccgtaaccg
      961 tgaggcacac cgtacgttca gggcctcttt tcggtcaaat tcgggaacag catgcgagcc
     1021 atggcgagga gacagcgtgt acggtggcac gagcttgtgg aaccacaagc tggaagcctt
     1081 ccggagggcc gaccggtgcg ggtgatggtc gggaaggtgg ccgtgtgtct ggtgcgctac
     1141 gaaggccggt tgttcgccct ggaggatgtg tgtccgcatc aaggccgcag cttcacgggg
     1201 ggaagctgcg agcgggggcg gatcatctgt ccctggcacc tcaagagttt cgatctgatc
     1261 accggccaag gcctcctggc gagcgaaggc aatgcccgtg tgttcccggt ggaagagaag
     1321 aacggccagg tgcgtatcgg catgccgcgc cggtcgttca gcctgttcgg cttgcgcctg
     1381 ggttgatcgg agcttcggga tcccgggtgg cgcacaatac gggccgatca caccgcgttc
     1441 aatagccgtg aaatgaaccc aaaaccccgc gcatgacgca ttctacactc cgcaaacgcc
     1501 ccgttcgccg ctctgccagc cccatgccaa ttctccaagg cccgcgccca tccacgatcc
     1561 acgccattct cagctacagc aaggcgctgc gcgtggtgga tgcgcctcct gtaggccagg
     1621 tgaacatcgt gctgaactga ggcggtgatc cgcacgggtg gcccggccgg tcctcatgtg
     1681 atcaccagca ggcgtgcagg ctccagccgg aagcgtacct cgttaccgat caccagcggc
     1741 tcgccatcga ggtgcgcgag gttgccatcc tggtgaatat gagcctcacg ggcaacgatc
     1801 gtgcggatgt atgggctgct gtccgccctg ccggtataca cgtcgaacag cgctttgatc
     1861 agcgggagga aggaaggctt gctcacgcac cgcagttcag ccagtccgtc atcgggtgct
     1921 gaaccgggac tgatggccac gccgttgccg aactcgcggg tgttcgcgaa gacgagcatg
     1981 agcagcctgt gttcggtgct ttcgttgttc gcgttcacca ccacgcgtag gggttgcgcg
     2041 ccgaggatct ccttcagcgt gatgcggagg tagttccaga aaccacgtga tcggctgcgg
     2101 tcgaattccc aagccacgcg cgcatcgaac ccgatgccgg ccgtcccgag gaagaggtgg
     2161 tcgttcaggt agcaaacgtc catcggtcgg gccgtaccgg tgaacgcgcg gtggagggcc
     2221 tccttcgggt tgagtggcag tttttgcgag cgggcgtagc cattgcccga tcccatggcc
     2281 acgatgccca gcgccaccgg cgagccgaca agtccattgg ctaccgcgtt caaggtaccg
     2341 tcgcccccag cggcgatcac acggtcatgc ccggcggtca cggcctcgcg ggccaattca
     2401 ctgccgtgcc cgggcccttc gatcatgcgc tcactgaggt ccaccccgag ctgcctggaa
     2461 agcgcacgcg cgagttccag cacgtccggg gcacggcctt tcccgctgcg ggggttcagg
     2521 atcagggtgg cgcgcatcat tcggtcacca ccatgtctcg tgcgagcagg tggttgttgt
     2581 actgctggat cgcggcctgt aacaaggcgt gcgcggtgat caggtcgaag gcgttcggga
     2641 tggtgcgctg ctccggatgc cacgagctgc cgatgccgtc gtgccagagt atccgttcgc
     2701 catcgctgcg caattgagca cgcggtgtca cgatgagcca ggtggcgctg ccctcgctca
     2761 cggcggccgg agaacgttcc aggcgcaggg tgctgctacc gaaagcgaag aatggttcgt
     2821 tgtggccgat caggtccagc acggtgggca ggatgtcgat ctgctgggtg gtgcgcacat
     2881 cggcacgggg aacgatcacg ccgggcatgt agtagaccag cgggatccag aagtcgtagg
     2941 ccgatccgct ctgttcgccg ttgcgctcca ggtccgccgt atggtccgcg gtgatcacga
     3001 agagggtgtt cttgaaccag gacatttttt ccgccgtgcg gaagaattcg cgcagggcat
     3061 tgtcggcata cagcagggtg gggtggatg
//
LOCUS       DAOQOC010000231         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16319_length_3088_cov_3.395318, whole genome shotgun sequence.
ACCESSION   DAOQOC010000231 DAOQOC010000000
VERSION     DAOQOC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16319_length_3088_cov_3.395318"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PL106_04860"
     CDS             <1..888
                     /locus_tag="PL106_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNA32429.1"
                     /translation="DKRFMAGLAYWLGLRAIGAGVVRSGPGDARGKWETVVECGVSTL
                     IAVPSFLLAMLREWEKHGLRADRTAIQRVVCIGEPIASTFGKPNLLAQRIADQCGFSL
                     HGTYAGTEMATACTEREPFEGHVVPTELLHVEVVDEQGAPVPEGGIGEVVATPFGVEA
                     MPLLRFRTGDICAWRMGTNEAGTPAMLLGPVLGRKEQRLKLKGTTLFPAQVTDAMNAL
                     PEPPEFVMVCVKDEYGGDELEILVDAEGAALGRLREHLRAALRVSPRLTTLPRQAIAT
                     MKWPPGARKPHFFIDRRDI"
     gene            976..1599
                     /locus_tag="PL106_04865"
     CDS             976..1599
                     /locus_tag="PL106_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32430.1"
                     /translation="MSALTLSIALVCTAPCTAQVTMADILQKKAPLTCVGLDFSEAHF
                     TPRFEFQKLEQRGSVFLNEWNNLIEAEPAKYDLGIPMGLDYVGNVTSYVLSANGNFQP
                     GKAFQAATGPLTDQQIASMVGKYPTKGVAGAGVVFLVNSFNKTAEQAQFTMVFFDMAT
                     HKVLHTERLQGKPGGFGLQNFWAGALVNVLKQIQTTYAPAWRVRYAQ"
     gene            1601..2869
                     /locus_tag="PL106_04870"
     CDS             1601..2869
                     /locus_tag="PL106_04870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA32431.1"
                     /translation="MHHSDVLVIGAGPSGTAAASWLAQRGHSVTVVDRAVFPRFVIGE
                     SLLPVSMGHWEETGQLAALKAQNYAIKAGANFYRNGKMFDLAFGENYTQGWTWTWQVP
                     RDHFDNVLAMEAQRLGARIHFGHEALRVDLEHGEGVELVTRHNGKEHHFTARFLIDSS
                     GYGGTLAKLLDLHDVPAGNGRMALFAHVRESEEHRGKYADPMRISFEVLAQDLWFWSI
                     PFSDGRTSLGFVGHHSHFDGPMGTGSGPTAMHRMLELPKAFSDRYQGVEFVHGPQVIR
                     EYTHYNQTLTGPRYVLTGNCAGFLDPVFSSGVAFATESGLLAAKLLDRQLKGEQVDWP
                     NEYEAYIRQGADVFRSYIETWYNGDLQDVFFADDVEQSHKERIISVLAGYVWDRTNPY
                     VSKHERAIPALAESLRLRDAERRKNLQRTT"
     gene            2871..>3088
                     /locus_tag="PL106_04875"
     CDS             2871..>3088
                     /locus_tag="PL106_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNA32432.1"
                     /translation="MTITEQKTDTDITASEARAAAQRIAFGPVLFQACQTMRRNGLFD
                     ALRRGRQAGSTEVELLAATALSPYGLRVL"
BASE COUNT          594 a          955 c         1009 g          530 t
ORIGIN      
        1 gacaagcggt tcatggccgg tctggcgtac tggctgggtc tgcgggccat tggcgcgggg
       61 gtggtgcgca gcggacccgg cgacgcgcgg ggcaagtggg aaacggttgt agagtgcggg
      121 gtatccaccc tgatcgccgt gccctcgttc ctgctggcca tgctgcgtga gtgggagaag
      181 cacggattgc gggccgatcg cacggccatc cagcgtgtgg tgtgcatcgg cgaaccgatc
      241 gccagcacgt tcgggaaacc gaatcttctg gcccagcgca tcgcggacca gtgcgggttc
      301 tcccttcacg gcacgtatgc gggcaccgag atggccaccg catgcaccga gcgcgaaccg
      361 ttcgaggggc acgtggtccc gaccgagctg ctgcatgtcg aagtcgtcga cgagcaaggc
      421 gctccagtgc cggagggcgg gatcggggaa gtggtggcca cgccgttcgg agtggaggcc
      481 atgcccctgc tgcgcttcag gaccggcgac atctgtgcct ggcgcatggg caccaacgag
      541 gcaggtacgc cagccatgtt gctcgggccc gtgctgggcc gcaaggagca gcgcttgaag
      601 ttgaagggca ccaccctttt tcccgcgcag gtgaccgacg cgatgaatgc cttgccggag
      661 ccgccggaat tcgtcatggt gtgcgtgaag gacgagtacg gcggtgatga attggagatc
      721 ctggtggacg cggaaggcgc cgctttgggg cggttgcgag agcatcttcg ggcagctttg
      781 cgcgtgtctc ctcggctcac cacattgccc cgccaggcga tcgccacgat gaaatggcct
      841 cccggggcgc gcaaaccgca cttcttcatc gatcggcgag atatttgagc ggtccttggc
      901 ggcgcggacg aaaagcgcca ctttcgcaac gaacgaacac cacgaacacc gatgatggat
      961 cctgttaccg ggcgtatgtc cgcccttacg ctgtcgatcg cgctggtctg caccgcaccc
     1021 tgtaccgccc aagtgaccat ggcggacatt cttcagaaaa aagcaccact gacctgtgtg
     1081 ggcctggatt tctctgaagc gcacttcacc ccccgtttcg aattccagaa actcgagcaa
     1141 cgcggctcgg tgttcctcaa tgaatggaac aacctgatcg aggccgagcc ggccaaatac
     1201 gatctaggga tccccatggg cctggattat gtgggcaatg tgacctcata cgtcctgagc
     1261 gcgaacggga acttccaacc cggcaaggcc ttccaggcgg ccaccggccc gctcaccgac
     1321 cagcagatcg ccagcatggt gggcaaatac cccacgaaag gagtagctgg cgccggcgtg
     1381 gtgttcctgg tgaacagctt caacaagacg gccgagcagg cgcaattcac catggtgttc
     1441 ttcgacatgg ccacgcacaa ggtcctgcac accgagcggc tgcaaggcaa gccgggaggg
     1501 ttcggcttgc agaacttctg ggccggtgcg ctcgtcaacg tgctcaaaca gatccagacg
     1561 acctatgccc cggcatggcg cgtgcgctac gcccaatagg atgcaccatt cggacgtgct
     1621 cgtgatcggt gccgggccgt ccggaaccgc tgccgcatcc tggctggccc agcgcggcca
     1681 ttcggtcacc gtggtggacc gcgctgtatt tccgcgcttc gtgatcggcg aaagcctgct
     1741 gccggtgagc atggggcatt gggaggagac cggccagctg gccgcgctga aagcccagaa
     1801 ttacgcgatc aaggccgggg ccaacttcta ccggaacggc aagatgttcg acctggcctt
     1861 cggagaaaat tatacccagg gttggacatg gacctggcag gtgcctcggg accacttcga
     1921 caacgtcctg gcgatggagg cccagcgcct cggcgcgagg atccatttcg gccacgaagc
     1981 gttgcgggtc gatctggaac atggcgaagg cgtggaactg gtgacacggc acaacggcaa
     2041 ggagcatcat ttcacggccc ggttcctcat cgacagcagc ggctacggcg gcacactggc
     2101 gaaactgctg gatctgcacg atgttcctgc tggcaacggc cgcatggcgc ttttcgcaca
     2161 tgtgcgcgag agcgaggagc accgcggcaa atacgctgac ccgatgcgta tcagcttcga
     2221 ggtgctggcg caggacctct ggttctggag catcccgttc agcgatgggc gcacctcgct
     2281 cggtttcgtt ggtcaccaca gccacttcga cgggcctatg ggcacgggct ccggaccgac
     2341 cgccatgcat cggatgctcg aactgcccaa ggccttcagc gaccggtacc agggtgtcga
     2401 gttcgtgcat ggaccgcagg tcatacgcga atacacgcac tacaatcaga cgctcacggg
     2461 gccccgatac gtgctcacgg gcaattgcgc cggcttcctg gacccggtgt tcagcagcgg
     2521 cgtcgccttt gccacggaga gcggactgct ggccgcgaag ctgctggacc ggcagttgaa
     2581 aggcgaacag gtggactggc cgaatgaata cgaggcatat atccggcagg gtgccgacgt
     2641 gttccgctcg tacatcgaaa cctggtacaa cggcgacctg caggatgtct tcttcgcgga
     2701 cgacgtggaa cagtcgcaca aggagaggat catctcggtg ctggccggct atgtctggga
     2761 ccggaccaac ccgtacgtca gcaagcacga gcgggcgatc cccgctctgg ccgagtccct
     2821 gcgcctgcgc gatgcggagc gccggaaaaa cctccagcga acgacctgaa atgacgatca
     2881 cagagcagaa gacggacacg gacatcaccg cctcggaagc gcgtgcggcc gcacaacgca
     2941 tcgccttcgg gcccgtactg ttccaggcct gccagaccat gcgcaggaat gggctcttcg
     3001 atgcgctgcg acgtgggcgt caggcaggaa gcaccgaagt ggaactcctg gccgccaccg
     3061 ccttgtcgcc gtacggcctg cgcgtgct
//
LOCUS       DAOQOC010000232         3082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16376_length_3082_cov_2.796829, whole genome shotgun sequence.
ACCESSION   DAOQOC010000232 DAOQOC010000000
VERSION     DAOQOC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16376_length_3082_cov_2.796829"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PL106_04880"
     CDS             complement(<1..493)
                     /locus_tag="PL106_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32433.1"
                     /translation="MDPLGGIRSQAAQAKGVQRNVKGQLKMAKAAQSKAAKLDRKLVL
                     HMLLFLLLGLVVGSWAFVSFFGEERGNVPKLVFGASVLGCVLLGMLHERWLVRRAEGE
                     RSLWPELLITLITMAFTGIGLWLGHHFFPILLGDPGQAVFALSMSWSAIGLLIPHLVL
                     LAFD"
     gene            complement(505..1449)
                     /locus_tag="PL106_04885"
     CDS             complement(505..1449)
                     /locus_tag="PL106_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNA32434.1"
                     /translation="MENWSIWKDLLEGGAEDLRPEVVVGELLISGVLSENDLFFIRNG
                     GTKRSVSRDIERVQLPGSTELRSERGMIELNRAGIYDGLPEGVFHQPKGSRPFRSIEE
                     VKEEFVENERIERDARKFFWPIDHELSWARVMIELNERRLTTDLLDDVTGRGVLRFWD
                     PPSYFSDEEKGDLLMLMPLCHRLVGDPVLSAQAMEEVLQVPVRLSTRIDERSLETDQD
                     LGMLSDMQLGVDSLLSGPATVLEQVLVARIGPMDAQVANTFAPGQLGRRKLDHLLGLL
                     TRADQLAQVEIVVTPAPAGARLATERGYCRLGVTSILN"
     gene            complement(1458..>3082)
                     /locus_tag="PL106_04890"
     CDS             complement(1458..>3082)
                     /locus_tag="PL106_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32435.1"
                     /translation="SDTRIFRRIAQYLLPDARLYARPAHGILQLDPAGAQDITRYDEL
                     SFEKQWRNKEDMNREDSIDLGFTATGTHTMDGLRIICRAQGSSLLGIERLRTPELTSL
                     GTTLAPGEVYLGLTGPTSEARSLTLYFDWLSDPQRDTWFRSLHKVTAFDMGNNSLSVG
                     RGPGSEEPVHDLRRTTNAIADIEEHTRLYYAERFLRIAFPTPLPAGAPPVVEQALART
                     NLAGIGNIRWVRLVFPSEFGPREIRDALILDNCVPVLNRRLQRTVFRLHREFNIKRLN
                     VEGFFQGIEKAESGSGQKYAEVPSLDMSEVTPGTYTMRQGTAGRFDDRDGTEFMNHML
                     DLLDEERRSFAAMDVNTTVSELRTIEQVLEKLGRRVQASGTGEGQRYIMIKPFTSSEN
                     AHVYFWTTDGEMADGLPVGTRFNCRNAGLSSTGQVTLVSTTTGARNELSPKETVQLYR
                     SMILSRGVLLSRQDIVEHCRYVGSSDIESVTVEEGVMASPDHGRGLLRCLDVNLKFLP
                     GRNDPEIVERVKRRITADLAASAGSSMPIRVN"
BASE COUNT          569 a          979 c          934 g          600 t
ORIGIN      
        1 catcaaaggc aagcaggacg aggtgcggga tcagcaggcc gatggccgac cagctcatgg
       61 aaagcgcgaa cacggcctgc ccgggatcgc ccagcagtat tgggaagaag tggtgaccca
      121 gccataggcc gatccccgtg aaggccatgg tgatcagggt gatcagcaac tccggccata
      181 aggaccgctc cccttcggca cgacgtacca gccacctctc atgcagcatg cccagcaaca
      241 cgcaccccag aacgctggcg ccgaacacga gcttgggcac gttcccgcgc tcttcgccga
      301 aaaaggacac gaacgcccat gatccgacca ccagccccag cagcaggaaa agcagcatgt
      361 gcagcaccag cttccggtcc agcttggccg ccttggactg cgccgccttg gccatcttga
      421 gttggccctt cacgttgcgc tgcacaccct tggcctgtgc ggcctggctc cgtatgcctc
      481 ccagcgggtc cattcaggtc aggttcagtt caggatggat gtgacgccta atctacaata
      541 tccgcgttcc gtggccagcc gtgccccagc gggcgcaggt gtaacgacga tctccacttg
      601 tgcgagctga tcggcccgtg tgagcagccc cagcagatgg tccaatttcc gacggcccaa
      661 ctggcccggt gcgaaggtgt tggccacctg tgcatccatc ggtccgatgc gggccaccaa
      721 tacctgttcg agtaccgtgg cgggcccgct cagcagactg tccaccccga gttgcatgtc
      781 cgacagcata ccgaggtcct ggtcggtttc aagggagcgc tcatcaatgc gcgtgctgag
      841 cctcaccggc acctgcaata cctcttccat ggcctgggca ctcaggaccg gatcgccaac
      901 caagcggtgg cacagcggca tcagcatcaa caggtcgcct ttctcctcgt ccgagaaata
      961 cgacggggga tcccagaaac gcaaaacccc acggccggta acatcgtcca acaggtcggt
     1021 ggtcagtcgg cgctcattca attcgatcat cacccgtgcc cacgacaatt cgtggtcgat
     1081 cggccagaag aatttccggg cgtcacgctc gatccgttcg ttctccacga actcttcctt
     1141 cacctcctca atactgcgga agggccgcga gcctttgggc tggtggaaaa cgccttcggg
     1201 caggccgtcg tagatccctg cccggttcaa ctcgatcatg ccgcgctcgc tccgcaactc
     1261 cgtgctgccg ggaagctgca cccgctcgat gtcgcggctc accgagcgtt tcgttccccc
     1321 gttgcggatg aagaagaggt cgttctcgct gagcacacca ctgatgagaa gttcgcccac
     1381 caccacttcg gggcggaggt cttctgcgcc tccctcgagc aggtctttcc agatgctcca
     1441 attctccatg gtggtcctca gttcactcgg atgggcatac tggagccggc agaagcggcc
     1501 aggtcagcag tgatgcggcg tttcacacgt tcaacgatct ccggatcatt acgcccgggc
     1561 aggaatttca ggttgacgtc caggcagcgc agcaggcccc ggccatggtc cggcgatgcc
     1621 atgacgccct cctccacggt gacgctctcg atatcgctcg aacccacgta acggcaatgt
     1681 tccacgatgt cctgtcggct gaggagcacc ccgcgtgaca ggatcattga ccggtaaagt
     1741 tgcacggtct ctttggggct cagttcgttg cgggcgcccg tggtggtgga caccaaggtc
     1801 acttggccgg tgctcgaaag accggcgttc ctgcaattga acctggtgcc cacgggcagg
     1861 ccatcggcca tttccccgtc ggtggtccag aagtacacgt gcgcgttctc cgaagaggtg
     1921 aagggcttga tcatgatgta ccgctgccct tcgccggtac cggacgcctg cacccggcgg
     1981 cccagtttct ccagtacttg ttcaatggta cgcaattcgc tcaccgtggt attcacgtcc
     2041 atcgcggcga acgagcgacg ctcctcgtcg agcaggtcga gcatgtggtt catgaactca
     2101 gtaccgtcgc gatcatcgaa tcgaccggcc gtgccctgtc gcatggtgta ggttccgggg
     2161 gtcacttcgg acatgtccaa cgagggcacc tccgcatatt tctggccgga tcccgactcg
     2221 gccttctcga tcccttggaa gaaaccctcc acgttcagcc gcttgatgtt gaactcccgg
     2281 tgcaacctga acacggtgcg ctgcaagcgg cggttcagta cggggacgca attgtccagg
     2341 atcagtgcgt cgcgtatttc ccgcggaccg aactcggagg ggaagaccag tcgcacccag
     2401 cggatgttcc cgatgccggc caggttggtt cgcgccaggg cctgttccac cacgggaggg
     2461 gcgccggcag gaaggggagt ggggaatgcg atgcgcagga agcgctccgc atagtacagc
     2521 cgtgtgtgct cctcgatgtc ggcgatggcg ttggtggtgc ggcgcaggtc atgaaccggt
     2581 tcttcggaac cggggccgcg gccaacggac aggctgttgt tgcccatgtc gaaggcggtc
     2641 actttgtgca gcgatcggaa ccaggtgtcg cgctggggat cgctgagcca gtcgaagtac
     2701 aaggtgaggg acctcgcttc gctggtgggg ccggtgaggc cgaggtacac ctcgcccggt
     2761 gccagtgtgg tgcccaatga ggtcagttcc ggtgtacgca ggcgttcgat acccaataaa
     2821 ctggatcctt gtgcacggca tatgatcctg aggccgtcca tcgtatgcgt gcccgtcgcc
     2881 gtgaagccca ggtcgatgga atcctcgcgg ttcatgtcct ccttgttgcg ccattgcttc
     2941 tcgaagctca gctcatcata gcgcgtgatg tcctgggccc ctgccgggtc cagttgcagg
     3001 atcccgtgcg cgggcctggc gtacaaccgg gcatcgggca gcaggtattg cgcaatgcgt
     3061 cggaagatcc tggtatccga gg
//
LOCUS       DAOQOC010000233         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16395_length_3080_cov_4.109752, whole genome shotgun sequence.
ACCESSION   DAOQOC010000233 DAOQOC010000000
VERSION     DAOQOC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16395_length_3080_cov_4.109752"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PL106_04895"
     CDS             complement(<1..631)
                     /locus_tag="PL106_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNA32436.1"
                     /translation="MGGFSEPVGFTFDANGRWYVWERGGKVWIVENGVKLSPPLIDLS
                     AEVGGWRDHGLLGFTLDPDFLTNGKIYLMYAVDRHHLMNFGTGNYNPATNQYYAATIM
                     RITRYTAVGPTFNSVDPASRTVLLGETRKTGAIMTHESHSTGQLCFGTDGTLLATIGD
                     GASYNSTDVGSDAGTYFAQALTDSIMRPKENVGALRSQLVDCFNGKLLRM"
     gene            989..2110
                     /locus_tag="PL106_04900"
     CDS             989..2110
                     /locus_tag="PL106_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32437.1"
                     /translation="MNLRHLLFLLPLTACTSQPDTTSSPPPAKAQAVPPVTEWNADSL
                     IGPGEKHFAHLRKLTWGGNNAEAYWNTRGTMLSFQSDFQGWGHSCDQIHAFDPFHDDL
                     RKGAPQQLSLNGGRTTCSYFLPGDSLVLFASTESGGAECPHTVSHTPGGKYLWNIFKE
                     YDIYVSDLKGHQRKQLTNSLGYDAEATVSPKGDRIVFTSMRDGDLDLYTMNIDGSDVK
                     RVTTDLGYDGGAFFSPDGTKLVWRASRPKTREEVKEYTDLLKQGLVQPTNMEVFIANA
                     DGTGAHAITALGKANWAPSWHPDGKRIIFASNHTTERGFPFTLYSINTDGTGLEQISY
                     TDAFDAFPMFSPDGKYLVFSSNRGGKDRETNLFLAEWKE"
     gene            complement(2244..>3080)
                     /gene="porQ"
                     /locus_tag="PL106_04905"
     CDS             complement(2244..>3080)
                     /gene="porQ"
                     /locus_tag="PL106_04905"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNA32438.1"
                     /translation="VQYINYGTFSRTDESGHETGSFTAGEYVLQVGGARAMDSLFRVG
                     VNLKYIISQLDSYKAGAIAADIGGVFEKKALGLTVAATIRNFGLMTRTYTGTKEKLPV
                     QVQLATTYRFRHAPFRLGLSIDNLQRWDLTYTDPHAPVQVDPTTGEAVVDKVSNAQKA
                     LWHIVPNLEVLFGKNFFLRFGYNYRRRQELNLPEKPGLAGMTAGLGLKVTKLHLSYGF
                     AQFYPGSASNTITLSVRFADWKKPTPEEQERIEQQKREKLEKKERKDMERKAKRAEEQ
                     EG"
BASE COUNT          590 a          971 c          902 g          617 t
ORIGIN      
        1 ccattcgcaa caacttgccg ttgaagcaat ccaccaactg cgaccgcaag gcgcccacgt
       61 tctccttggg ccgcatgatc gaatcggtga gcgcctgcgc gaaatacgtg cctgcgtcgc
      121 tgcccacatc ggtactgttg tagctggccc catccccgat cgtggcgagc agcgtgccat
      181 ccgttccgaa gcacaattgg cccgtactgt gcgactcatg cgtcatgatc gcaccggtct
      241 tgcgcgtttc gcccagaagc acggtacggc tggccggatc caccgaattg aacgtggggc
      301 cgaccgccgt atagcgcgtg atgcgcatga tggtggccgc gtaatactgg ttggtcgcgg
      361 ggttgtagtt gcccgtaccg aagttcatga ggtggtgccg gtccaccgcg tacatcaggt
      421 agatcttccc gttggtcagg aagtccgggt ccaacgtgaa acccagcagg ccatggtcgc
      481 gccagccgcc tacctcggcc gagagatcaa tgagtggagg gctcagtttc accccgttct
      541 ccacgatcca caccttgccg ccccgttccc acacgtacca gcgcccgttc gcatcgaaag
      601 tgaagcccac cggctcgctg aatcctccca tcacttgcga agccgtgaaa cccgcaggta
      661 ccgtttgggc ttgcactggc tgctcgatac ttgaaagcag cccggaaaac aacgcaacga
      721 gggttgaccg ggagagaacg acgcgtatgt tcatggagtg cgcaccttgt ttggccgacg
      781 aaagtacctg atccatcgag gtatggcaag catctcccct tccggatccg cccccctccg
      841 gcgtttcgga ccgttcgtac gcctttccga taaggcaggt ttatggcgat cccatgacga
      901 tccgcctcat gaggcggtac ggccgacggg tagtttcgcg gccgctcagc cggggtatcc
      961 ccggtgtgta acgtcccgct taccccacat gaacctccgg catctccttt tcctcctccc
     1021 cctcacggcc tgcaccagcc agcccgatac cacttcgtcc ccacctccgg ccaaggccca
     1081 ggcagtgcca ccggtcactg aatggaatgc ggattcactg atcggtccgg gcgaaaagca
     1141 tttcgcacac ctgcgcaagc tcacctgggg cggtaacaac gccgaggcgt attggaacac
     1201 ccggggtacg atgctgagtt tccaaagcga tttccagggc tggggacaca gttgcgacca
     1261 gatccatgcc ttcgatccct tccacgacga cctgcgcaag ggtgcgccgc agcaactcag
     1321 cctgaatggt ggccgcacca cgtgcagcta tttcctgccg ggggacagcc tggtgctctt
     1381 cgccagcacc gagagcggcg gtgccgaatg tccgcacacc gtgtcgcaca caccgggggg
     1441 caagtacttg tggaatatct tcaaggagta cgacatctat gtgagcgacc tgaagggtca
     1501 ccagcgcaag caactcacca acagcctggg ctacgatgcc gaggccaccg tttcgcccaa
     1561 gggtgaccgc atcgtgttca ccagcatgcg cgatggcgat ctggacctgt ataccatgaa
     1621 catcgatggc agcgatgtga agcgggttac caccgacctg ggatacgatg gtggtgcatt
     1681 cttcagccca gatggtacca agctggtctg gcgggcctca cggcctaaga cacgcgagga
     1741 agtgaaggag tacaccgacc tgctgaagca aggtctggtg cagcccacga acatggaggt
     1801 gttcatcgcc aacgccgatg gtacgggggc gcatgcgatc accgcgctgg gcaaggccaa
     1861 ttgggccccg agctggcacc ccgatggcaa gcggatcatc ttcgccagca accacaccac
     1921 cgaacgcggc ttccccttca ccttgtacag catcaacacc gacggcaccg gcctggagca
     1981 gatctcctac accgatgcct ttgacgcgtt ccccatgttc agcccggatg gaaaatacct
     2041 ggtattcagc agcaatcgtg gcggcaagga ccgggagacc aacctgttcc tggccgaatg
     2101 gaaggagtag acccgggtca gcacgcgttc catagcaggg atgcgtggga gcactgggat
     2161 ggcgatcccg ccggaacgat ccggctggat cgtcatttca accctgaggt acttcggtag
     2221 agtccccgtg catctaccgg ctttcaccct tcctgctcct cggcgcgctt cgctttccgc
     2281 tccatgtcct tgcgctcctt cttctccagc ttctcacgtt tctgctgctc gatgcgctcc
     2341 tgctcctcgg gtgtgggctt cttccaatcc gcgaagcgca cggacagggt gatcgtattg
     2401 ctggctgagc cagggtagaa ttgtgcgaaa ccgtagctca aatgcagttt ggtcaccttc
     2461 aggcccaggc ctgcggtcat gccggcgagc cccggttttt cgggcaggtt gagttcctgc
     2521 ctccggcggt agttgtagcc gaagcgtagg aagaagttct tgccgaagag gacttccagg
     2581 ttcggaacga tgtgccagag cgctttctgt gcattgctca ccttgtccac cacggcctcg
     2641 ccggtagtgg ggtccacctg cacgggggcg tgcgggtcgg tgtaggttag gtcccagcgt
     2701 tgcagattgt cgatcgaaag gcccaagcgg aagggtgcgt gccggaagcg gtaggtggtg
     2761 gccaattgca cttggaccgg cagtttctcc ttggtccccg tgtacgtacg cgtcatcaac
     2821 ccgaagttgc ggatggttgc tgccacggta aggcccaagg ccttcttctc gaacacgccg
     2881 ccgatgtccg cagcgatagc acctgccttg tagctgtcca gttgcgagat gatgtatttg
     2941 aggttgacgc ccacccggaa caggctgtcc atggcacgtg cgccaccgac ctggagcaca
     3001 tactcccctg cggtgaagct cccggtctca tggcccgatt catcggtccg ggagaacgtg
     3061 ccgtagttga tgtactgcac
//
LOCUS       DAOQOC010000234         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16428_length_3077_cov_4.620781, whole genome shotgun sequence.
ACCESSION   DAOQOC010000234 DAOQOC010000000
VERSION     DAOQOC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16428_length_3077_cov_4.620781"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PL106_04910"
     CDS             <1..396
                     /locus_tag="PL106_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32439.1"
                     /translation="DFFQNYTASVLRSPVFQPTPESRTYFIDAFRAQQYLAGGARAIM
                     AVARNRFDLRLEGYVFQPYKTIVRASGDAAVSGTAISDRYFIASGSLIWQSPIGPVWF
                     NTSYLDGLPKPWAFSLNFGYIIFARKALG"
     gene            complement(495..818)
                     /locus_tag="PL106_04915"
     CDS             complement(495..818)
                     /locus_tag="PL106_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNA32440.1"
                     /translation="MADDKNQPRPGNQLNIEISEEVADGIYSNLAIITHSNSEFVVDF
                     VRVMPGVPKAKVRSRILLTPQHAKRLMRALMDNVQKFESAHGPIRESDMPEIPMHFGG
                     PPAQA"
     gene            complement(902..>3077)
                     /locus_tag="PL106_04920"
     CDS             complement(902..>3077)
                     /locus_tag="PL106_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNA32441.1"
                     /translation="LMDRIKSDNQGFNSIYMMMDSGARGSKEQIRQLSGMRGLMAKPQ
                     KSGGGGGQDIIENPILSNFKEGLSILEYFISTHGARKGLADTALKTADAGYLTRRLVD
                     VAQDVVITNDDCGTLRGLVASALVKNEEIVETLHDRILGRSAVHDVYHPQTGDLLVAS
                     GDQITEDVAKAIGESPIEEVEIRSVLTCEQRHGVCAKCYGRNLATGRMVQMGEAVGVI
                     AAQSIGEPGTQLTLRTFHVGGVAGKITEDSQITAKYDGKLEIDELRTVKKKDKKGEEA
                     EVVISRSTEMRIVDNNTGIVLTTSNIPYGAMLYKVSGKNVKKGDLICNWDPYNAVIIS
                     EHPGTLAFESIEDGVTFREQIDEQTGFTEKVIVESRDKKKNPMIRIVDGKSKEDLKSY
                     SLPVGAHIVVNEGQKINAGDILVKIPRSSGKGGDITGGLPRVTELFEARNPSNPAVVS
                     EIDGIVSFGKIKRGNREIIVESRTGEHKYYLVPLSKHILVQENDFIKAGQPLSDGSIS
                     PGDILDIQGPTRVQEYLVDEVQEVYRMQGVKINDKHFEVIVRQMMRKVEIEDPGDTRF
                     LERQAINKADFMEENDEMYDKMVVTNAGDSTVLKEGQIVTARKLRDENSALKRKDQKV
                     AEARQATPATSRQLLMGITRASLQTDSFISAASFQETTKVLNEAAVSAKEDNLKGLKE
                     NVIVGHLIPAGTGSRRFQKNLVYNKEDYTKLLAERMATQEVE"
BASE COUNT          485 a          968 c          917 g          707 t
ORIGIN      
        1 gatttcttcc agaactatac cgcctcggta ctgcgctcac ccgtgttcca gcccacgccg
       61 gaaagccgca cgtacttcat tgatgccttc cgcgcccagc agtacctggc cggtggggcc
      121 agggccatca tggcggtcgc tcgcaaccgg ttcgacctgc gcctggaggg ctatgtcttc
      181 cagccgtaca agaccatcgt gcgtgccagc ggcgacgccg ccgtttccgg taccgccatc
      241 agtgatcgtt atttcatcgc gagcgggtca ctgatctggc aaagccctat cgggccggtc
      301 tggttcaaca cgagctacct ggacggcctg ccgaagccgt gggcgttcag cctgaatttc
      361 gggtacatca tcttcgcacg gaaagcgctg ggatgaccgg tcggtcatca ggtgcccgcc
      421 ttcggccggc agtgccgggt gcgaatgcga aagcccccgg aattccaggg gctttcaact
      481 gaaagggatg cgctttaggc ctgcgccggc gggcctccga agtgcatggg gatctccggc
      541 atgtcgctct cacggatagg cccgtgcgcg ctctcgaact tctgcacatt gtccatgagc
      601 gcgcgcatga ggcgcttggc atgttgtggt gtgaggagga tgcgcgaacg caccttggct
      661 ttgggcacgc ccggcatcac acgtacgaag tccaccacga actcggagtt gctgtgcgtg
      721 atgatggcca ggttgctgta aataccatcg gccacctcct cactgatctc gatgttcagc
      781 tggttgcccg gccggggctg gttcttatcg tctgccatga tgtttgcgaa tgatcggtgt
      841 cgatcgtgcg tgcctcctca acggagcatt caccacacgt cgccatcgcg ttcggttcgt
      901 gtcactccac ctcttgcgtg gccatgcgtt cggccagcag cttggtatag tcctccttgt
      961 tgtacacgag gttcttctgg aagcggcggc tgccggtacc agcggggatg agatgaccga
     1021 cgatcacgtt ctccttgagg cccttgaggt tgtcctcctt ggcgctcacg gcagcctcgt
     1081 tgagcacctt ggtggtctcc tggaaggagg cggcgctgat gaagctgtcg gtctgcagcg
     1141 aggcacgggt gatgcccatg aggagctggc ggctggtggc cggtgtggcc tggcgggcct
     1201 cagcgacctt ctggtccttg cgcttcaagg cgctgttctc gtcgcggagc ttccgtgcgg
     1261 tgacgatctg gccctccttc agcacggtgc tgtccccggc gttggtgacg accatcttgt
     1321 cgtacatctc gtcgttctcc tccatgaaat ccgccttgtt gatggcctgg cgttccagga
     1381 aacgggtatc gcccggatcc tcgatctcca ccttgcgcat catctggcga acgatcactt
     1441 cgaagtgctt gtcgttgatc ttcacaccct gcatgcggta cacctcctgc acctcatcca
     1501 ccaggtattc ctgcacgcgg gtgggtccct ggatgtccag gatgtcaccc gggctgatgg
     1561 aaccatcgct cagcggttgt cccgccttga tgaagtcgtt ctcctgcacg aggatgtgtt
     1621 tgctcagcgg cacgaggtag tatttgtgct cgccggtgcg gctctcgacg atgatctcgc
     1681 ggttgccgcg cttgatcttg ccgaagctca cgatgccgtc gatctcgctc accacagcgg
     1741 ggttgctggg gttacgtgcc tcgaagagct cggtgacccg gggcaggcca cccgtgatgt
     1801 cgccgccttt gccactgctg cgggggatct tcaccaggat gtcgccggcg ttgatcttct
     1861 ggccctcgtt caccacgatg tgcgcaccaa cggggagcga gtagctcttg agatcctcct
     1921 tgctcttgcc gtccacgatg cggatcatcg ggttcttctt cttgtcgcgg ctctccacga
     1981 tcaccttctc ggtgaagccg gtctgctcgt cgatctgctc gcggaaggtg acgccgtcct
     2041 cgatgctttc gaaggccagc gtaccggggt gctcgctgat gatcacggcg ttgtacggat
     2101 cccagttgca gatcaggtcg cccttcttca cgttcttgcc ggacaccttg tagagcatgg
     2161 ccccgtacgg gatgttgctc gtggtgagca cgatgcccgt gttgttgtcc acgatacgca
     2221 tctccgtgga gcggctgatc accacttcgg cctcttcgcc cttcttgtcc ttcttcttga
     2281 ctgtacgcaa ctcgtcgatc tcgagcttgc catcgtactt ggcggtgatc tggctgtcct
     2341 cggtgatctt cccggcaaca ccacccacgt ggaaggtccg gagcgtaagc tgcgtaccgg
     2401 gttcaccgat ggactgcgcg gcgatcacgc caacggcctc gcccatctgc accatgcggc
     2461 ccgtggccag gttgcggccg tagcacttgg cgcacacacc gtgccgttgc tcgcaggtga
     2521 gcacgctacg gatctccacc tcctcgatgg ggctctcgcc gatcgccttg gcaacatcct
     2581 cggtgatctg atcaccgctg gccacgagca gatccccggt ctgcgggtga tacacgtcat
     2641 gcacggcact acggccgagg atccgatcat gaagggtctc cacgatctcc tcattcttca
     2701 ccagagcgct ggccaccaga ccgcgcagtg tgccgcagtc atcgttggtg atcaccacgt
     2761 cctgcgccac gtccaccaga cgacgggtga ggtaaccggc gtcagcggtc ttcagcgcgg
     2821 tatcggcgag acctttacgt gcaccgtggg tggaaatgaa gtactccagg atggacaagc
     2881 cctccttgaa gttcgagaga atggggttct cgatgatgtc ctgaccgccg ccaccgccac
     2941 tcttctgcgg cttggccatc agtccgcgca tgccgctgag ctgacggatc tgctctttgg
     3001 aaccacgggc accgctgtcc atcatcatgt agatggaatt gaagccctga ttatcgctct
     3061 tgatccgatc catcagg
//
LOCUS       DAOQOC010000235         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16646_length_3055_cov_3.668000, whole genome shotgun sequence.
ACCESSION   DAOQOC010000235 DAOQOC010000000
VERSION     DAOQOC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16646_length_3055_cov_3.668000"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PL106_04925"
     CDS             <1..559
                     /locus_tag="PL106_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32442.1"
                     /translation="DNSPAVRDPYRGIPCLGGAADLDAWLKARNGDLPDFIVSAGHTH
                     GRARLAIHADLEARGLRPATAIHRTAFVAATAAVSEGSMIFAQASLCAEARAGRCCII
                     NTRASVDHESVLEDGVSVGPGAIITGLVHVGRCADIYSGAVILPRLRIGEGAVVGAGA
                     VVRQDVPPRTLVAGNPARIIRSLEV"
     gene            556..1605
                     /locus_tag="PL106_04930"
     CDS             556..1605
                     /locus_tag="PL106_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32443.1"
                     /translation="MKCLLLAPTRYPFIRSIGAGLHANGLEVVQVDFRDLFGTTTNAL
                     NDKYSSLPRRVRRLWEDPYVRRTNQEYLRIFRELKPDLVFIYNDQLLRPETVNAFKEQ
                     ARVAFYLGDHPLYTPTNPYNLSILSASSCTITPDSFWCEQLTRMGLKNTVVDHFGINP
                     EVHHPVEPTAEQRADLGADLVYIGSASKTNWGYKRARFLDLFSGMDLRAWITGPMDRW
                     YREFPALATKVRPHARFDAAFNNLVYNCAKLAPVELVPSLFHGIHVRVFDVLGAGIMP
                     LCEYSRDLEAVFGETGMPLIRDYREAADLARHWIGADDERKGTVERMRAVAIARYAPE
                     LVIRRLLERIFPAWH"
     gene            1596..3026
                     /locus_tag="PL106_04935"
     CDS             1596..3026
                     /locus_tag="PL106_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32444.1"
                     /translation="MALSLAKLAGLLLAAIGATYFAPPMVASAFYLLATAAYILDKDE
                     PVWLTFFFVLSDGTLGLFNNYEAVIAVIPGLPEVEVGHLYVLATVYKALRIPARTPPP
                     FYRTAGIVFLLYTLFLVVQGYAVGVTQDIRFHFRIVKYILPLLLFLSLPRLMPGRDDH
                     ERTFMLFVPFAFLAFLAQVYSLVMGIAPAQAFGIHRESGLLDAYARGNTYRGFYSTGM
                     VLISFFGALWIGTVHAGWRRWLCLAVVIADVLSAFLSATRGWVLAFGMSLLLYAVFVA
                     RYRVGQVLGGSLLVAATFLLLMRVPAVERQFSKASERILTIGLVAQGDVTAGGTLERL
                     DRRAPRVMKKWSGSPLTGVGFSNDYREYADTHVGNQNLLLHGGIVGVAIMAAFLGYFC
                     WALVARGLSLPVTDPLHRGLLTFPIFLLAWFIIHSTSGQQFMFAGEPGITIPQALFFT
                     FGAVCHREAGRVIDAGRSRVEQAMSA"
BASE COUNT          474 a         1042 c          915 g          624 t
ORIGIN      
        1 tgacaactca cctgccgtgc gcgatccgta caggggcatc ccctgcctgg gcggcgcagc
       61 tgatctggac gcttggctga aggcccggaa cggcgacctg ccggacttca tcgtgagtgc
      121 gggacacacc cacggccgtg cgcgcttggc catccacgcg gacctggagg cgcgcggcct
      181 gcgaccggcc acggccatcc accgcaccgc cttcgtggcc gccacggccg cagtgagcga
      241 gggcagcatg atcttcgccc aagcctcgct gtgcgccgag gcccgcgcgg gtcgctgttg
      301 catcatcaat acccgcgctt cggtggatca cgagagcgta ctggaggacg gggtctccgt
      361 ggggcccggt gccatcatca ccggtctggt gcacgtggga cgttgcgctg atatatactc
      421 cggagcggtg atcctgcccc gattgcgcat cggcgagggc gccgtggtgg gggccggtgc
      481 cgtggtgcgg caggatgtcc cgccccgcac cctggtcgcc ggcaacccgg cccggatcat
      541 ccgatcattg gaggtatgaa gtgcctgttg ctcgcgccga cccgctaccc cttcatccgt
      601 tcgatcgggg cggggctgca cgccaatggc cttgaagtgg tgcaggtgga cttccgcgac
      661 ctcttcggca cgaccaccaa cgccctcaac gacaagtaca gctcgttgcc acgccgcgtg
      721 cgtcggctct gggaggatcc gtacgtacgc cggacgaacc aggagtacct ccgcatcttc
      781 cgggaactga agccggacct ggtgttcatc tacaacgacc agctgctccg gccggagacc
      841 gtcaacgcct tcaaggagca agcccgcgtg gccttctacc tgggcgatca tccgctgtac
      901 acgcctacca acccttacaa cctgagtatc ctgtccgctt cctcctgcac gatcaccccc
      961 gacagtttct ggtgcgagca gttgacgcgc atggggctca agaacacggt ggtggaccat
     1021 ttcggtatca atcccgaagt gcatcatccg gtggaaccga ccgctgagca gcgcgctgat
     1081 ctcggagccg acctggtgta catcggcagt gcgagcaaga ccaactgggg ctacaaacgt
     1141 gcccgcttcc tggacctctt cagcggcatg gacctgcggg cctggatcac cggccccatg
     1201 gaccgctggt accgcgagtt ccccgccctt gccaccaagg tgcggccaca tgcccgtttt
     1261 gatgcggcct tcaacaacct ggtgtacaac tgcgccaagc tcgccccggt ggaactggta
     1321 ccatccctgt tccatgggat ccatgtgcgc gtgttcgatg tgctgggcgc cggtatcatg
     1381 ccgttgtgcg agtacagcag ggacctggag gcggtcttcg gggaaaccgg tatgccattg
     1441 atccgcgatt acagggaggc ggcggacctt gcccgccact ggatcggcgc cgatgacgag
     1501 cgcaagggca ccgtggagcg catgcgtgcc gtggccatcg cccgttacgc tcccgagctg
     1561 gtcatccgcc gcctgctcga acgtatcttc cccgcatggc actgagcctc gccaagctcg
     1621 ccgggctgct cctcgccgcc atcggggcca cctacttcgc gccgcccatg gtggcttcgg
     1681 ccttctattt gctggccacg gccgcgtaca tcctcgacaa ggatgagccg gtctggctca
     1741 cctttttctt cgtgctgtcc gatggaaccc tcggtctctt caacaactac gaggccgtga
     1801 tcgccgtcat tcccggtctt cccgaagtgg aggtcggcca tctctatgtg ctcgccaccg
     1861 tgtacaaggc cctgcgcata ccggcccgta cgccacctcc tttctaccgg acggctggaa
     1921 ttgttttcct cctctatacg ctcttcctcg tggtgcaggg ctatgcggta ggcgtcaccc
     1981 aggacatccg cttccacttc cgcatcgtga agtacatcct gccgctcctg ttgttcctct
     2041 ccctgcctcg attgatgccc ggtcgcgatg atcatgagcg caccttcatg ctcttcgtcc
     2101 cgttcgcgtt cctggccttc ctggcacagg tgtacagcct ggtcatgggc atcgctcccg
     2161 cccaggcctt cggtatccac cgcgagtcgg gcctgctcga cgcgtacgca cggggcaata
     2221 cataccgtgg attctacagt acgggcatgg tgctcatctc gttcttcggc gcgctctgga
     2281 tcggcacggt acacgccggg tggcgccgct ggctctgcct ggccgtggtg atcgccgacg
     2341 tcctcagtgc attcctttcc gctacccgcg gatgggtgct ggccttcggt atgtccctgt
     2401 tgctatatgc ggtgttcgtg gcccgctacc gcgttggtca ggtcctcggt ggaagcctgc
     2461 ttgttgccgc caccttcctg ctcctgatgc gtgtacccgc ggtagaacgg cagttcagca
     2521 aagcctccga aaggatcctc accatcggac tggtggccca gggcgatgtg accgcgggtg
     2581 gcacgctcga acgcctggac cgccgcgccc cgcgcgtgat gaagaagtgg agcggatcac
     2641 ctttgaccgg cgtcggtttc tccaacgact accgggaata cgccgacacc catgtgggca
     2701 accagaacct gctgctccac ggcggcattg ttggcgtggc catcatggca gccttcctcg
     2761 gttatttctg ttgggcgctg gtcgcgcggg gtctctcact tcccgtgacc gatccgctgc
     2821 accgtggcct gctcacgttc ccgatcttcc tgttggcctg gttcatcatc cactccacct
     2881 ccggtcagca gttcatgttc gccggcgaac cgggcatcac cataccacag gcgctcttct
     2941 tcactttcgg tgcggtatgc caccgtgagg ccggccgggt gatcgatgcc ggaagatcaa
     3001 gagttgaaca ggccatgtcc gcatgatcac ggccgaggac catcgacccc tgcat
//
LOCUS       DAOQOC010000236         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16694_length_3051_cov_3.147530, whole genome shotgun sequence.
ACCESSION   DAOQOC010000236 DAOQOC010000000
VERSION     DAOQOC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16694_length_3051_cov_3.147530"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PL106_04940"
     CDS             <1..331
                     /locus_tag="PL106_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNA32445.1"
                     /translation="HKLDAPSGTAITLARDIDLKTQRYSGHILTEAMYLPEHQEGTPT
                     PVPVRSERTGEVPGKHSITWTSPEDRITITHDAFGRQGFATGAVVAAEWVRGRRGLFT
                     MADVLGG"
     gene            440..2002
                     /locus_tag="PL106_04945"
     CDS             440..2002
                     /locus_tag="PL106_04945"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNA32446.1"
                     /translation="MIPWILLLAYLVVLFVCLPKIFAKAGTPAWAGWIPGYNLFVWNR
                     ILGKPWYWIILLLVPGINLLMLIIYNVNTSIAFNERTLKQHVIAVLTPWVTLPRLAFK
                     EQHKWVGPLPVGQRKRGLAGQWGDAILFAVIVATTFRTFTFEAFTIPTPSMEKSLLTG
                     DYLFVSKLSYGPRMPMTPITFPFTHHTLPMTKSVPSFTTWFSLPYFRLPGLGEPERGD
                     AVVFNFPEGDTVVVGYDNPSYYQWERQTGRRNILERHFVDYWSDGQQRARIPIDGITI
                     RPTDKQENYIKRCVGVAGDTIEVRNGDLYVNGTLSPLPVMGQYAYDIVSKGSLVKERL
                     KDDLDVNKEEYDNGYRSGRGLALTAEGVEKLKANKNVTEVRRRIKPKGSYKATDFWPI
                     FPNDSAYNWTEDNFGPVWVPKAGATVKLTLANLPLYRRAIQAYENNTLEVRGGDIFIN
                     GAKTDSYTFKQDYYWMMGDNRHRSQDARFWGFVPFDHVVGKAVLVWLTADPEKGGFPM
                     GIRWKRLFSLVR"
     gene            2002..2988
                     /gene="lepB"
                     /locus_tag="PL106_04950"
     CDS             2002..2988
                     /gene="lepB"
                     /locus_tag="PL106_04950"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNA32447.1"
                     /translation="MDGDDTSQGQGRPGLVMRVLARWRGLNEWLRALIIAFAILVFTH
                     TFLLGWVTVRSTSMYATLHPGDLTAVTKWPAWAGWDRGDIVVFHDPMQDDRKLGERQL
                     MVKRVVGLPGDVVELRDGELLVNNVPVPPWPGETRSWLVRLRKGTDAHALLNELHLPV
                     SGSPSDTRELELPLNEKLALTLRGREDVLGVERMRNARGYAAHLFPFSPYHRWNTDDY
                     GPIIIPKRGDHVKVDTRSLPLYDRVITRYEGNDAEAVRAAIPAENGDQVTYTMRQDYY
                     FVLGDSRHHSDDSRHWGFLPADHLVGRAWFVLLGHDLNQGGIRDDRWFTGVR"
BASE COUNT          609 a          978 c          935 g          529 t
ORIGIN      
        1 ccacaagttg gacgcaccca gtgggacggc catcaccctg gcacgcgaca tcgatctgaa
       61 gacgcagcgg tacagcgggc acatcctgac agaggccatg tacttgccgg agcaccagga
      121 gggcacaccc acaccggtac ctgtccggag tgagcgaaca ggcgaggtac cgggcaagca
      181 cagcatcacg tggacgagcc ccgaggaccg gatcaccatc acgcacgacg ccttcgggcg
      241 ccaaggcttc gccacgggtg ccgtggtggc cgccgaatgg gtgcgcggcc gcaggggcct
      301 gttcaccatg gcggacgttc tgggcggctg atcgcctctt cacgcatttt cacggacgcg
      361 cttcgcccct attgcgcacc ttcgcgcacc acaattccag aagtcccatg ggcacatgcc
      421 cgcatgggcg cacgcgcaca tgatcccctg gatcctcctc ctcgcctacc tcgtcgttct
      481 gttcgtttgc ctgccgaaga tcttcgccaa agccggaaca ccggcgtggg cgggctggat
      541 accgggctat aaccttttcg tttggaaccg gatcctgggc aagccgtggt actggatcat
      601 cctcctgctg gttccgggca tcaacctgct gatgctgatc atctacaacg tgaacacgag
      661 catcgccttc aacgaacgca cgctgaaaca gcacgtgatc gcggtgctca caccatgggt
      721 caccttgccg aggctggcct tcaaggagca gcacaagtgg gtgggcccgc tcccggtggg
      781 ccagcgcaaa cggggcctgg ccgggcagtg gggcgatgcg atcctcttcg cagtgatcgt
      841 ggccaccacg ttccgcacct tcaccttcga ggccttcacc atccccacgc catccatgga
      901 gaaaagcctg ctcaccggcg attatctttt tgtgagcaag ctgagctacg gtccgcgcat
      961 gccgatgacg ccgatcacct tccccttcac acaccatacg ttgccgatga ccaagtcggt
     1021 gcccagcttc accacctggt tctccctccc ctacttccgc ctgccggggc ttggcgaacc
     1081 cgagcgtggc gatgcggtgg tcttcaactt ccccgaaggc gataccgtgg ttgtgggata
     1141 cgacaacccc agctactacc aatgggaacg ccagaccggc cgccgcaaca tcctggaacg
     1201 gcacttcgtg gactactgga gcgatggtca gcaacgtgcg cgcatcccca tcgatggcat
     1261 caccatccgc cccaccgaca agcaggagaa ctacatcaag cggtgcgtgg gcgtggccgg
     1321 ggacacgatc gaggtgcgca acggggacct gtacgtgaac gggacgctgt cgccgctacc
     1381 ggtgatgggt caatacgcgt atgatatcgt atcaaaggga tcattggtga aagagcgctt
     1441 gaaggacgat ctggacgtga acaaggagga atacgacaac ggctaccgca gcgggcgcgg
     1501 gctggccctc accgccgagg gtgtggaaaa gctcaaggcc aacaagaacg tcaccgaggt
     1561 acgtcgccgc atcaaaccca agggctcgta caaggccacg gacttctggc ccatcttccc
     1621 caacgacagc gcctacaact ggacagagga caacttcggg ccggtgtggg tgcccaaggc
     1681 aggcgccacg gtgaagctga cactcgcgaa cttgcccttg taccggcggg ccatccaggc
     1741 gtatgagaac aacaccctgg aagttcgcgg tggcgacata ttcatcaatg gtgcgaaaac
     1801 cgacagctac accttcaagc aggactacta ctggatgatg ggcgacaacc gccaccgctc
     1861 gcaggacgcg cgtttctggg gtttcgtacc cttcgaccac gtggtgggca aggccgtgct
     1921 ggtgtggctc accgccgatc ccgaaaaagg aggattcccc atgggcatcc gttggaagcg
     1981 gctgttctcc ctggtacgct gatggacggc gacgacacgt cccagggcca aggccgaccg
     2041 gggcttgtca tgcgcgtgct ggcccgatgg cggggactga acgaatggct gcgggcgctg
     2101 atcatcgcct ttgccatcct ggtgttcacg cacacgttcc tgctgggttg ggtcaccgtg
     2161 cgcagcacca gcatgtacgc caccctgcac cccggcgatc tgaccgcggt gacgaaatgg
     2221 ccggcctggg ccggttggga ccggggcgat atcgtcgtct tccacgaccc catgcaagat
     2281 gatcggaagc tgggtgaacg ccaattgatg gtgaaacggg tcgtaggtct gcccggagac
     2341 gtggtggaac tgcgcgatgg tgaactcctc gtgaacaacg tgccggtacc cccgtggccc
     2401 ggagaaaccc gaagctggct cgttcgcctg cggaaaggca ccgatgccca cgccctgttg
     2461 aacgagctgc acctgccggt cagcgggtcc ccttcggaca cgcgcgagtt ggagcttccc
     2521 ctgaacgaaa agctcgcgct cacgctgcgc gggcgggagg acgtgctggg cgtggaacgc
     2581 atgcggaacg cccggggcta tgccgcccac ctcttcccat tcagtccgta ccaccgctgg
     2641 aacaccgacg actacgggcc gataattata cccaaaagag gagatcatgt gaaagtggac
     2701 acgcgctcgc tccccttgta tgaccgcgtc atcacccgct acgagggcaa cgatgcggaa
     2761 gccgttcgtg cggcgatacc ggctgagaac ggcgaccagg taacatacac catgcggcag
     2821 gattattact tcgtgctggg cgacagtcgg caccacagcg acgacagtcg gcactggggc
     2881 ttcttgccgg ccgaccacct cgttggccgg gcgtggttcg tgctcctggg ccacgatctg
     2941 aaccagggtg gaattcgcga tgaccgttgg ttcaccggcg tgcgctgatc ccatcaccgg
     3001 ctgctcgaac gcaccggaga cgtgcgcgag ctcggagacg atctggctca t
//
LOCUS       DAOQOC010000237         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16733_length_3048_cov_3.236218, whole genome shotgun sequence.
ACCESSION   DAOQOC010000237 DAOQOC010000000
VERSION     DAOQOC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16733_length_3048_cov_3.236218"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PL106_04955"
     CDS             complement(<1..683)
                     /locus_tag="PL106_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA32448.1"
                     /translation="MSAKIVHRCGDGPSGLHAVVLSRSFATLVPSARHRMKNAFQDRR
                     TLVLVIVASLGYFVDIYDLVLFNVVKKESLEAIGLAGDTLARFDTSLFNWQMGGMLLG
                     GLLWGILGDKRGRVQVLFGSILLYSVANIANAFVTGTAAYSFCRLIAGIGLAGELGAG
                     ITLVVESMGRTQRGWGTMIIVTVGALGAVAASFVGKEGAAIADALGLPLQGWQMAYVV
                     GGALGLVLLV"
     gene            682..1575
                     /locus_tag="PL106_04960"
     CDS             682..1575
                     /locus_tag="PL106_04960"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HNA32449.1"
                     /translation="MILRFLPLLLLACGASRSPAPGPSLLPYDLDHPSARFTLPDDLV
                     EISALTDVDSSTVACVQDERGAIYFISLRDGRKIREVPFSGPGDMEGLTRVGGDYFAL
                     RSDGLVFRLRERHTAPDRMDVLDTFRIDVPNRNVEGLGYDDRDKRVLVSPKDLIKGPK
                     EVRDERVIHGFSPDDPAHRVQEVLRLSVEELVAQARVSGIPVPERTTDKGRVVPALKL
                     RYSSVAVHPMTGHYYLLSAVDRTLLVVDRAGKLIALEQLDEELLPKPEGITFLTNGDL
                     VLSSEGKGGTPVIVRYAYLLN"
     gene            complement(1885..>3048)
                     /locus_tag="PL106_04965"
     CDS             complement(1885..>3048)
                     /locus_tag="PL106_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32450.1"
                     /translation="PTSLLEVNSGAGDAVFGHSTNVGGYLGRETNISFGTPLQTIQGA
                     GLYANNPAAGYTSMYTQSTGAATVAASVNFSDVWIASYNYVQNGLAGFNPSASYSQLN
                     VTNPALGGVQAAFRSYSDRGTAAGNPGFTVGEQATANAQNQDAFAVVGSAFSNSLTRA
                     GGYFESYDYGGLANAYAYVGTAVTGVLRKITGTNAVSEIVPTAKHGRIMLTCPESPEY
                     WYQDYGTVQLVNGHAHVDLDPILADIIMVNDEYPARVFCTPVDMLAFNGVAQMNRTAT
                     GFDLVELNGGTHSGTLDYQLVVKPKTGFGEGRFPQAPGPAWLKKDLEPASARAANQPD
                     PSKVFYWPSDHEVYGYDVDKVTPVGASVAAGPNAGKMKVAEGVIMDHYPAERP"
BASE COUNT          575 a          906 c          987 g          580 t
ORIGIN      
        1 accagcagca ccagtccgag cgcgccgccg accacgtacg ccatttgcca gccttgcagc
       61 ggcaggccga gcgcatcagc gatggcggcg ccctctttgc ccacgaaact ggcggctacc
      121 gcccccaagg ccccaacggt cacaatgatc atggtgcccc agccgcgctg cgtacgcccc
      181 atgctctcca ccaccagggt gatccctgcg ccgagttcac cggccagtcc gatcccggcg
      241 atcaggcgac agaacgaata ggccgcggtg ccggtgacga acgcgttggc gatgttggca
      301 acggagtaga gcagaatgga gccgaagagt acttgcacgc gcccgcgttt gtcgcccagt
      361 atgccccaga gcaggccgcc gaggagcatg ccacccatct gccagttgaa gagcgaagtg
      421 tcgaaacgcg caagcgtgtc accagcaagt ccgatggctt ccagactttc ctttttcacc
      481 acgttgaaca gcaccaggtc gtagatgtca acgaagtagc ccaaggaggc cacgatcacc
      541 aggacgagcg tgcggcggtc ctggaaggcg ttcttcatgc ggtggcgggc actgggtacg
      601 agggtggcga aggaacgcga aagcacaacg gcgtgcaagc ccgatggtcc gtcaccgcat
      661 cggtgaacga tcttcgcgct catgatcctc cgcttcctgc cgctgctcct gctcgcctgc
      721 ggtgcatcgc gatccccggc cccgggtcct tcgttgttgc cctacgacct ggatcaccca
      781 tccgcgcggt tcacccttcc ggatgatctg gtggagatct cggcgctcac cgatgtggac
      841 agcagcacgg tggcgtgtgt ccaggacgag aggggggcca tctatttcat ctccttgcgg
      901 gatggtagaa aaattcgcga ggtgccgttc tccggaccgg gcgacatgga aggactgacc
      961 cgggtgggcg gcgactattt cgcattgcgc agcgacggcc tcgtgttccg gttgcgggaa
     1021 cggcacacgg caccggaccg catggacgtg ttggacacct tccggatcga tgtgccgaac
     1081 cggaacgtcg aagggctggg atacgatgac cgggacaagc gtgtgctcgt atccccgaag
     1141 gacctcatca agggcccgaa ggaagtgcgt gatgagcgcg tgatacatgg cttttcaccg
     1201 gatgaccccg ctcatcgcgt gcaggaggta ttgcgactct cggtggagga gctggtcgct
     1261 caggcacgtg tgtcgggtat tccggtgccc gagcgcacga ccgacaaggg tcgcgtggtg
     1321 cctgcactca aattgcggta ttcatccgtg gcggtgcacc cgatgacggg gcactactac
     1381 ctgctgtccg ccgttgaccg gacactgctc gtggtggatc gcgcgggcaa gctgatcgcg
     1441 ctggaacaac tggatgagga actgctgccc aagcccgaag gcatcacctt tttgaccaat
     1501 ggcgatctgg tgttgtcgag cgaaggcaaa ggtggtactc cggtgatcgt gcggtacgcg
     1561 tacttgctga actgataccg attcgagaag gaatgaggtc agggcctggt ctccagtgca
     1621 ttgcgtagat ggcttggacg atgcgccgca cgggtacgtt gttcgacttg ctgttgcgcg
     1681 gaccgtcgat ctctcggcga tgagttcaag gtgggcatca aatggaatat gaacatcacg
     1741 gcggcaacgg cccaccagcc gggtgcgaag gcggacccga gcagggtatg gatggtggtg
     1801 atgctcttca gcgggtgagg atccggcatg cgctgccagt gtcgatccgt gctgcctgaa
     1861 aaatgccgac gaaggtgcgc cggttcaagg gcgttccgca gggtagtggt ccatgatgac
     1921 gccctcggcc accttcatct tgccggcgtt cggcccagcg gccacgcttg cgcccacagg
     1981 ggtcaccttg tccacgtcgt agccatacac ctcgtggtcg ctgggccagt agaagacctt
     2041 cgaggggtcg ggctggttcg cggcgcgtgc cgatgcgggt tccagatcct tcttgagcca
     2101 ggccggacca ggtgcttggg ggaagcggcc ttcgccgaag cccgttttcg gcttcaccac
     2161 gagctggtag tccaaggtgc cggagtgggt acctccattg agttcaacca ggtcgaatcc
     2221 ggtggcggta cggttcatct gggccacccc gttgaatgcg agcatgtcca ccggtgtgca
     2281 gaacacgcgg gccggatact cgtcgttcac catgatgatg tccgccagga tcggatccag
     2341 gtccacatgg gcatgtccgt tgaccagttg aacggtccca tagtcttggt accagtactc
     2401 cggcgattcc gggcaggtga gcatgatgcg gccgtgcttg gcagtgggta cgatctcgct
     2461 cacggcgttg gtaccggtga tcttccgaag cacaccggtg accgccgtgc ccacatacgc
     2521 atacgcattc gcaagcccgc catagtcgta cgattcgaag tagccaccag cgcgcgtgag
     2581 tgaattggag aaagcggagc ccactaccgc gaacgcatcc tggttctgcg cattggccgt
     2641 ggcctgctca ccaaccgtga agccggggtt gcccgccgca gtgccacgat cgctgtacga
     2701 ccggaaggcg gcttgtacac caccgagtgc aggattcgtc acgttcaatt gcgaatagct
     2761 ggcggacgga ttgaacccgg cgaggccgtt ctgaacgtag ttgtacgagg cgatccatac
     2821 atcgctgaag ttgaccgagg ccgcaacagt ggccgcacct gtggattggg tgtacatgga
     2881 ggtatagccg gccgccggat tgttggcgta gagaccggcc ccctgaatgg tctgtagtgg
     2941 agttccgaac gagatgttcg tttcgcgtcc gaggtaacca cccacgttgg tactgtgccc
     3001 gaagacggca tcgcccgcac cactgttcac ctccagcagg gaggtagg
//
LOCUS       DAOQOC010000238         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16831_length_3040_cov_3.697152, whole genome shotgun sequence.
ACCESSION   DAOQOC010000238 DAOQOC010000000
VERSION     DAOQOC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16831_length_3040_cov_3.697152"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1348)
                     /locus_tag="PL106_04970"
     CDS             complement(68..1348)
                     /locus_tag="PL106_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNA32451.1"
                     /translation="MEHSPLAERMRPHDLDEVVGQEHLVGPNGILRKALSGGRLPSMI
                     LWGPPGVGKTTLARLVAERMKRPFHALSAINSGVKDVREVIEQASGAGLFTRSAVLFI
                     DEIHRFSKAQQDSLLGAVEKGTVTLIGATTENPSFEVISALLSRCQVYILEPLDEAQL
                     QKLLQRAMSDDAALKRIPIELRETDALMRASGGDARRLLNTFELCVQAAWTAGTGTAV
                     ITDGTVKEVVLTHAARYDKKGEQHYDIISAFIKSLRGSDPNASVYWLARMIEGGEDPV
                     FIARRMVILASEDIGNANPNALLMATTTMQAVQMIGWPEGRIILSQCAVYLACSPKSN
                     ASYKAIGAAQAEVKRSGDLPVPLHLRNAPTKLMKDIGYGTGYRYSHDQPPQDADQEFL
                     PGAISGMRLYEPGDNPREREYAAMIERIFKNRYQ"
     gene            complement(1456..2301)
                     /locus_tag="PL106_04975"
     CDS             complement(1456..2301)
                     /locus_tag="PL106_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32452.1"
                     /translation="MRSCRWLAGPVLCISLTIAAQSDTTARVALSQALDALKEARRDG
                     QRDTINAAIKHHLRSVLEQDDAFTTDLGVPPLSLLDAPDGKFRLLTWNIPYADGSNLF
                     EGFLLVRDKHRHVLYELRDMTPRIENAAYKMLGPDYWYGALYYAVIPVRRGAKTWYTL
                     LGWKGYSNVETRKVIDVLGFKGAVPQFGAPLFGEDRVKRTREIFGFSFQSSMSLKWEK
                     EFNRIVVDHLAPMKPEFQGQAAFMGPDLSYDAYVWEKDHWSYQRDVDVRQQDMNRPWN
                     RPPKE"
     gene            complement(2506..2853)
                     /locus_tag="PL106_04980"
     CDS             complement(2506..2853)
                     /locus_tag="PL106_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNA32453.1"
                     /translation="MAIIITDECINCGACEPECPNNAIYEGGAEWRLSDGTNLHGMQT
                     TPMGNTWDADMAQTPKSMDVHYIVPDKCTECTGFHDEPQCASVCPVDCCVDDPDFRET
                     KEELMAKKEFMHL"
BASE COUNT          520 a          974 c          924 g          622 t
ORIGIN      
        1 catcatttac gacctgcaca agagcttcct ggagaacgaa cgcgcgaaat gaggccggag
       61 cggccgttca ctggtacctg tttttgaata tccgttcgat catggcggcg tactcccgtt
      121 cgcgcgggtt gtcccccggc tcgtaaagcc gcatgccgct gatggccccc ggcaggaact
      181 cctggtccgc atcctgtggc ggctggtcgt ggctgtaccg gtagcccgtt ccgtagccga
      241 tgtccttcat cagcttggtg ggcgcgttgc gcaggtgcag gggcaccggc aggtcgccgc
      301 tgcgcttgac ctcggcctgg gctgcaccga tggccttgta cgaggcgttg ctcttgggtg
      361 agcaggccag gtacacggcg cactggctca ggatgatgcg gccttcgggc cagccgatca
      421 tctgcaccgc ctgcatggtg gtggtggcca tcagcaacgc gttcgggttg gcgttgccga
      481 tgtcctcgct ggccaggatc accatgcgcc gtgcgatgaa caccgggtcc tcgccgccct
      541 cgatcatgcg cgccagccag tatactgaag cattgggatc gctgccacgc aggctcttga
      601 tgaaggcgct gatgatgtcg tagtgctgct cgcccttctt gtcgtaccgt gccgcatggg
      661 tgagcacgac ctctttcacc gtgccgtccg tgatcacggc cgtgcccgtg ccggccgtcc
      721 aagccgcctg cacgcacaac tcgaaggtgt tcaacagccg tcgtgcatct ccaccactgg
      781 cccgcatcag ggcgtccgtc tcccgaagct cgatggggat ccgcttcaga gcggcgtcat
      841 cgctcatggc acgctggagg agcttctgca gctgggcctc gtccaatggt tccaggatgt
      901 acacctggca gcgggagagg agcgcgctga tcacctcgaa ggaggggttc tcggtggtgg
      961 cacctatcaa ggtgacggta cctttttcaa ccgccccgag gagcgaatcc tgctgggctt
     1021 tgctgaagcg gtggatctcg tcgatgaaga gcaccgcact gcgcgtgaag agcccggccc
     1081 cgctggcctg ttcgatcacc tcgcgtacgt ccttcacccc gctgttgatg gcgctcaagg
     1141 catggaaagg ccgcttcatc cgctccgcca ccaggcgggc cagggtggtt ttgcccacac
     1201 ctggcgggcc ccacaggatc atgctcggca ggcgcccccc cgacaacgct ttgcgcagga
     1261 tgccattggg ccccaccaga tgctcctgac ccaccacctc atccaggtcg tgcggccgca
     1321 tccgttccgc cagcgggctg tgctccatgt tcaggtcaca aaggaaacaa gtggggcggt
     1381 ccgggccgac gcgcgcacgt cctgcgcagg aacgctgttg ctatcggtgt ccgacttatc
     1441 gttcgcggcc ccggatcatt ccttcggcgg acggttccat ggccggttca tgtcctgctg
     1501 ccgtacgtcc acatcacgtt ggtacgacca gtggtccttc tcccacacat acgcgtcgta
     1561 actgaggtcc ggccccatga aggccgcctg cccctggaac tcgggcttca tgggggcaag
     1621 atggtcgacc acgatgcggt tgaactcctt ttcccacttc agactcatgc tgctttggaa
     1681 gctgaagccg aagatctccc gggtgcgctt cacgcggtcc tcgccgaaga gcggcgcacc
     1741 gaactgtgga accgcaccct tgaagccgag cacatcgatc accttgcggg tttccacgtt
     1801 gctgtagccc ttccagccca ggagcgtgta ccaggttttc gcaccccgcc tcaccggtat
     1861 caccgcgtaa tacaacgcgc cgtaccagta gtcgggcccg agcatcttgt acgcggcgtt
     1921 ctcgatgcgg ggcgtcatat cgcgcagctc gtacagcacg tgccgatgct tgtcgcgcac
     1981 aaggaggaac ccctcaaaaa ggttcgaccc gtccgcgtac gggatgttcc atgtgagcag
     2041 ccggaatttc ccatcggggg catcgagcag gctcaacggc ggaacgccca ggtcggtggt
     2101 gaacgcatcg tcctgttcca ataccgaacg caagtgatgc ttgatggccg cattgatcgt
     2161 gtcgcgctga ccgtcgcgcc gggcctcctt caatgcatcc aacgcttgcg aaagcgcaac
     2221 cctggccgtg gtgtcggact gtgccgcgat cgtcaggctg atgcagagga cggggccggc
     2281 cagccatcga catgaacgca tgtgacgaag gtacggcaca cctggatggg gacagccgta
     2341 catggtgctt gttccgggtc tcttcacggg cgccctccgg gctattcccc gatcatgctt
     2401 cgccgaacaa tggtcattca gccttcgcca cgctaccgtg gtccggcttc acctgaacga
     2461 tccacggcct cggaggtcat cctgcaggag aacctgttga ggcgcttaca gatgcatgaa
     2521 ctccttcttg gccatcagct cctccttggt ctcgcggaag tcggggtcgt ccacacagca
     2581 atcaaccggg cataccgacg cgcactgtgg ttcatcgtgg aaaccggtgc attcggtgca
     2641 cttgtccgga acgatgtagt ggacgtccat ggacttgggc gtttgtgcca tgtcggcgtc
     2701 ccacgtattg cccatcgggg tggtctgcat cccgtgcagg ttcgtaccat cgctcaagcg
     2761 ccattcggca ccaccttcgt agatcgcgtt gttcggacat tccggctcgc atgcgccaca
     2821 attgatgcac tcgtcggtga tgatgatggc catagcaggg ggctattttc ggccgcgaat
     2881 ttagaacgca tgagcagtgc agcaccggga accctccaga aggtccctct ttcggaccgg
     2941 ataaaggcgt tcgagcaact gggcgaactc ttcgccctgt gggggaccag caccccctgg
     3001 cccggccatg gttgcggttt gaacaagccg gagtatgatg
//
LOCUS       DAOQOC010000239         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16843_length_3039_cov_3.506367, whole genome shotgun sequence.
ACCESSION   DAOQOC010000239 DAOQOC010000000
VERSION     DAOQOC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16843_length_3039_cov_3.506367"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PL106_04985"
     CDS             <1..631
                     /locus_tag="PL106_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32454.1"
                     /translation="PFSIQVTSLSHLRYFSRLSVDARWSAIYDKRKHRVTLRGFAGMF
                     LNKAQYMPSAMGWRMYWGSSDLLHDNLFIDRQAPGRNTAQQMAKVQGGFKTPTSYGTS
                     DSWIAALNLEADLPFRIPLSVFASYGAAPYTIVAQQGQRTDWKGYWEAGFGVRLVRDV
                     VEMWIPLVYSKEIRDEVEGFRGFDFGERIRFVLALEKMDPTQLLRKAPH"
     gene            642..1451
                     /locus_tag="PL106_04990"
     CDS             642..1451
                     /locus_tag="PL106_04990"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNA32455.1"
                     /translation="MKPGQRVHFLSDFHLGIPDAASSLVRERRICAFLDEAAKDASEI
                     FLLGDLFDFWFEWRKAVPRGHVRLLGKLAELTDRGIPVHLFLGNHDMWIFDYVPKETG
                     VIVHREPIEREINGKRFLIGHGDGLGPGDHGYKFMRSIFRNPVCQWLFARLHPNFALW
                     LGDFWSGRSRKKSYENDRKWLGEDKEWLVQYCRGQLEKKHFDFMLFGHRHLPVDIDVE
                     AAGRRGRYINLGDWISYFTYAVFDGHEVELKRRVGDGPLCDDVRVSGGPAV"
     gene            complement(1533..2525)
                     /gene="gap"
                     /locus_tag="PL106_04995"
     CDS             complement(1533..2525)
                     /gene="gap"
                     /locus_tag="PL106_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNA32456.1"
                     /translation="MRIAINGFGRIGRVTARILQQRNDIELVAVNDLTDTRTLAHLFK
                     YDSVHGVFSGEVGHDPEHLLLNGRKVKAFAQKDPAALPWKQLGIDVVIESTGLFLTRE
                     AATMHLKAGAKRVLLSAPAKDPDIRTVVLGVNDHVIDPADPVLSNASCTTNCAAPMIL
                     GVQELCGIEDGFITTVHSYTGDQRLHDAPHKDLRRARAAAVSMIPTTTGAAKAITRIF
                     PELDGRLGGGGIRVPVPDGSITDITVRVKNLKSAGEINAYFKQLAEGRLKGILRYTED
                     PIVSVDIVSDPHSCIFDAQLTSVVGNMVKIMGWYDNEYGYSSRLVDLVGRLGKL"
     gene            complement(2583..>3039)
                     /locus_tag="PL106_05000"
     CDS             complement(2583..>3039)
                     /locus_tag="PL106_05000"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNA32457.1"
                     /translation="TKLETLIPDFKGEWHNLATVLDTRPDVLSHNLETVRRLTKLVRV
                     QARYDRSLEVLLRAKRAGLRTKSGVMLGLGERDHEVLETMDDLRSVGCDVLTLGQYLQ
                     PTQRHLPVVEFVHPDTFARFREEGLARGFRFVESGPLVRSSYHAEKHVL"
BASE COUNT          574 a          909 c          977 g          579 t
ORIGIN      
        1 cccgttcagc atccaggtga cgagcttgag ccacctgcgt tacttctcgc gcttatcggt
       61 ggatgcgagg tggagcgcga tctacgataa acgcaagcac cgtgttaccc tgcgcggttt
      121 tgccggcatg ttcttgaaca aggcccaata catgccatcc gccatgggct ggcggatgta
      181 ttggggcagc agcgatctgc tgcatgacaa cctcttcatc gaccggcagg cccctggccg
      241 caacacggcc caacagatgg ccaaggtgca aggaggcttc aaaacgccca catcgtacgg
      301 caccagcgac agttggatcg cggcgttgaa cctggaggcc gatctcccgt tccgcattcc
      361 cttgagcgtg ttcgccagct acggcgccgc cccgtacacg atcgttgctc agcagggcca
      421 gcgaacggac tggaaaggat actgggaagc tggctttggt gtccgattgg tgcgcgatgt
      481 tgtggagatg tggatacccc tggtgtacag caaggagatc agggacgagg tggaagggtt
      541 ccggggcttc gacttcggcg aacgcatccg cttcgtactg gcactggaga agatggaccc
      601 cacacaactg ctgcgcaaag ccccgcactg accacgaccg catgaaaccc ggccagcgcg
      661 tccacttcct ctccgacttc cacctcggca tacccgatgc ggcgagcagc ctggtgcggg
      721 agaggcggat ctgcgccttc ttggacgaag cggccaagga cgcatcggag atcttcctgc
      781 tgggcgacct gttcgatttc tggttcgagt ggcgcaaagc cgtgccgcgt ggccatgtcc
      841 ggcttctggg caaactggcc gagctgacgg accgggggat ccccgttcat ctcttcttgg
      901 ggaaccacga catgtggatc ttcgactatg tgccgaagga aaccggcgtg atcgtccacc
      961 gcgaaccgat tgaacgcgag atcaacggga agagattcct catcggccat ggtgatgggc
     1021 tgggacccgg cgaccacggg tataaattca tgcggtcgat cttccgcaac ccggtatgcc
     1081 aatggctgtt cgcacggctg catcccaact tcgcactgtg gctgggtgac ttctggagcg
     1141 ggcgcagccg gaagaagagc tacgagaacg accgcaagtg gcttggggag gacaaggaat
     1201 ggctggtaca atattgccgc gggcaactgg agaagaagca cttcgacttc atgttgttcg
     1261 gtcatcgcca cctgcctgtc gacatcgatg tggaggcggc cggtcgtaga gggcgctaca
     1321 tcaacctggg cgattggatc agctacttca cctacgccgt gttcgatggc catgaggtgg
     1381 aactgaagcg gcgcgtgggc gatggaccac tgtgcgacga cgtccgtgtg agcggtggac
     1441 cagcggtgtg aacaggatcg gatcccacga caacgcacca agaggagagc gatggccggg
     1501 cgctggagca ggtcgcaacc cgagcggtcg gatcacaact tccccaaccg gcccaccagg
     1561 tccacgagcc tggaactgta cccgtactcg ttatcgtacc agcccatgat cttcaccatg
     1621 ttgcccacca cgctggtgag ttgcgcatcg aagatgcagc tgtgcgggtc cgagacgata
     1681 tccacgctca cgatcgggtc ctcggtatag cgcaggatcc ccttcagccg accttcggca
     1741 agctgcttga aatacgcgtt gatctccccg gcgcttttca aattcttcac ccgaacggtg
     1801 atgtcggtga tggagccatc gggcacgggc acacggatac cgccaccgcc gagccggcca
     1861 tcgagttcgg ggaagatgcg cgtgatggcc ttggccgcgc cggtggtggt ggggatcata
     1921 ctgacggccg ccgcccgggc ccggcgcagg tccttgtgcg gcgcatcgtg caggcgctgg
     1981 tccccggtgt agctgtgtac cgtggtgatg aagccgtcct cgatgccgca cagctcctgc
     2041 acgccgagga tcatgggcgc tgcacagttg gtggtgcaac tcgcattgct gaggaccggg
     2101 tcggcggggt cgatcacatg gtcattcacg ccgagcacca ccgtccggat gtccggatcc
     2161 ttcgccggcg cagagagcag gacccgcttc gctccggcct tgaggtgcat ggtggccgcc
     2221 tcgcgggtga ggaacagccc cgtggactcg atcaccacgt cgatacccaa ttgcttccat
     2281 ggcagtgctg cgggatcttt ttgagcgaag gccttcactt tgcgcccgtt gagaagcagg
     2341 tgttcggggt cgtgccccac ctcaccgctg aacacaccat ggaccgaatc gtacttgaag
     2401 aggtgggcca gggtccgcgt atcggtcagg tcgttcacgg cgaccagctc gatatcattc
     2461 cgttgttgga ggatccgggc ggtgactcgg ccgatccttc cgaagccgtt gatggcgatg
     2521 cgcatggtca attgcgggtc aaggtggagg cacgggatac gatacggttg aagacaccac
     2581 catcaaagca catgcttctc ggcgtggtag ctgctacgca cgagcgggcc gctctccacg
     2641 aagcggaaac cgcgtgccag gccctcctca cggaatcgtg cgaaggtgtc cggatggacg
     2701 aactccacca cgggcaggtg gcgctgtgtg ggctgaaggt actgccccag ggtgagcacg
     2761 tcgcagccca cgctgcgcag gtcatccatg gtttccagca cctcatggtc gcgttcgccg
     2821 aggccgagca tcacaccgct cttggtgcgc agtccggcgc gcttggcgcg aagaagcact
     2881 tcgaggctcc gatcgtagcg ggcttggaca cgcaccagct tggtcagccg ccggaccgtt
     2941 tcgaggttgt ggctcaggac atcggggcgc gtgtcgagca cggtcgccag gttgtgccat
     3001 tcccccttga aatcggggat caatgtttcg agcttggtc
//
LOCUS       DAOQOC010000240         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16846_length_3039_cov_3.091823, whole genome shotgun sequence.
ACCESSION   DAOQOC010000240 DAOQOC010000000
VERSION     DAOQOC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16846_length_3039_cov_3.091823"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PL106_05005"
     CDS             <1..997
                     /locus_tag="PL106_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA32458.1"
                     /translation="WQFMIGTGKLYGLRSDSYVDERCDVYKSTDAACRYLKDLYRIFG
                     SWELALAAYNCGPGNVNKAMRRSGGKDYWGVYDRLPRETRGYVPAFIAVNYLFHHAAD
                     HNLYPIMPNYCAYEVDTVQVNYPVDLAKMAALLGGNPQELLDLNPVYKRGHVPNTGET
                     ATLYLPRTLVAAFIDREDTIRNSWTPEVLSTAPVAQVAAPDQHNTASSQRTHIVRRGE
                     SLGSIAQKHDVTVKQLRTWNGLHSDRIHPGKRLIVRMATAPSRTASVPDTPVEQGADA
                     ATEIILHTVQPGDTLWGIAKRYPGATVDGIRRINDVSDVEELKVGKKIKVAVQKG"
     gene            1221..1451
                     /locus_tag="PL106_05010"
     CDS             1221..1451
                     /locus_tag="PL106_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32459.1"
                     /translation="MEITRIRPYVHKHCKFKLRSGKEVFGVIWEVDGLDKRSLFFASI
                     GDYERLQRDPSKPVSVINLRPEEIMHVESIAS"
     gene            complement(1536..>3039)
                     /locus_tag="PL106_05015"
     CDS             complement(1536..>3039)
                     /locus_tag="PL106_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNA32460.1"
                     /translation="TFFYSRKDKTLRNFKIFRHVLGTDERLDVEVFHEKDDAFSCDVY
                     RDRSNAFVMISTGSTLSTEEWYLPVGDPNGKFTVILPREEEHEYSVQHANGEFYIMTN
                     WKATNFRYMKCPVKSTAKEAWVEVVPHREDVLLEDVDLFKDHMVFSERKDGLTRLRVR
                     KLSTGAEHPISFNDPAYVAYTGVNPEFDSDKLRYGYTSLTTPNGVYEHNMDTAKDTLL
                     KQQEVVGTFHASDYVSERVWAPARDGKRVPVSIVYRKGTRIDGPSPMLLYGYGSYGHS
                     MDPNFSSARLSLLDRGFVFALAHIRGGEELGRAWYDDGKMEHKMNTFTDFIDCAEYLL
                     KNKYADPGRLFCMGGSAGGLLMGAVVNMRPDLWNAVVAEVPFVDVVTTMLDDSIPLTT
                     GEFDEWGDPKEKEAYERMLSYSPYDNVKDAAYPAMLVLTGFHDSQVQYWEPAKWVQRL
                     REHQKATAPIILKTNMEAGHGGASGRFERLKEVALIYAFLIQRAGLGTTP"
BASE COUNT          593 a          976 c          858 g          612 t
ORIGIN      
        1 ctggcagttc atgatcggca ccggcaagct gtacggcctg cgctccgaca gttacgtgga
       61 tgaacgctgc gatgtgtaca agagcaccga tgcggcctgc cgctacctga aggacctgta
      121 ccgcatattc ggtagctggg agctggcctt ggccgcgtac aactgcggtc ccggcaacgt
      181 gaacaaggcc atgcgccgat cgggcgggaa ggattactgg ggcgtgtacg atcgcctccc
      241 gcgtgaaaca aggggctatg tgccggcctt catcgcggtg aactacctct tccaccatgc
      301 ggcggaccac aacctctacc cgatcatgcc caattattgc gcgtacgagg tggacaccgt
      361 gcaagtgaac tatccggtcg acctggccaa aatggcggca cttctcggtg gcaacccgca
      421 ggagttgctt gacctgaacc cggtgtacaa acgcggccac gtgcccaaca ccggcgagac
      481 cgccaccctg tacctgccac gcacgctggt ggccgccttc atcgatcgcg aggacaccat
      541 ccgcaatagc tggacccccg aggtgctatc cactgcaccg gtggcacagg tcgcagcccc
      601 cgatcagcac aacaccgctt cctcccaacg cacacacatt gttcggcggg gcgaatcact
      661 cggctcgatc gcccagaaac acgatgtgac tgtcaagcaa ctgcgcacgt ggaacggtct
      721 gcacagcgac cgcatccatc ccggtaagcg cctcatcgtt cgcatggcca ccgcgcccag
      781 ccgaacggca agcgtgcccg acactcccgt tgagcagggg gccgacgctg ccacggagat
      841 catccttcat accgtgcagc cgggcgatac cctttggggc atcgcaaagc gctatcctgg
      901 tgcaactgtg gatgggatcc gccgcatcaa tgatgtatcg gatgtggagg agttgaaagt
      961 gggcaaaaag atcaaggtcg cggtgcaaaa aggttgaccg gtcgacgacc cgtgcctaca
     1021 ccgaagggcg cattttccac agacccgctc cgaggcaccg cagcgccttc gctttgacca
     1081 tcaggcaata gctgccacct ccgcaacgtt gtcattgggc gctgtcgacc gttgttgata
     1141 attcaacgtg catcacgagt cggtcgtcgt ctattttgcg atgcctgtcc ggacgacttt
     1201 cccgtcgatc ggcccctgcc atggagatca cacgcatccg cccgtatgtc cacaagcact
     1261 gcaaattcaa gctgcgcagt ggcaaggagg tttttggtgt gatctgggaa gtggatggcc
     1321 ttgacaagcg ttcgcttttt ttcgccagca tcggcgacta cgaacgcctt cagcgggacc
     1381 cgagcaagcc cgtgagcgtg atcaacctcc gcccggagga gatcatgcac gtggagagca
     1441 tcgcgagctg agcccttgtt ccttcgcacc gcagcgcctc gctggcccgc tgcgtcgtcg
     1501 atccaggtcg aacttcaggg ttgttgtacc cgttctcaag gagtcgtgcc aagtcctgcc
     1561 cgttggatca ggaacgcata gatcaatgcc acctccttca gccgttcgaa acgtccgctg
     1621 gcgccaccat ggccggcctc catattcgtc ttgaggatga tcggggcagt ggccttctgg
     1681 tgctcgcgca gccgctgcac ccatttggcc ggctcccagt actgcacctg gctgtcgtgg
     1741 aaaccggtga gtaccagcat cgcgggatac gccgcatcct tcacgttatc gtacggggag
     1801 tatgacagca tgcgttcgta cgcctccttc tccttggggt cgccccactc gtcgaactca
     1861 cccgtggtga gcgggatgct gtcgtccagc atcgtcgtca ccacatccac gaagggcact
     1921 tcggccacca cggcgttcca gaggtccggc cgcatgttga cgaccgcgcc catgagcaaa
     1981 ccgcccgcac tgccccccat gcagaacagt cgaccggggt ctgcatactt gttcttgagc
     2041 agatattctg cacaatcgat gaagtccgtg aaggtgttca tcttgtgctc catcttccca
     2101 tcgtcgtacc aggcgcggcc aagctcctcg ccgccgcgga tgtgcgcgag tgcgaacacg
     2161 aaaccacggt ccagcaggga aagtcgggcg ctgctgaagt tcgggtccat gctgtggccg
     2221 taggaaccgt acccgtacag gagcatgggt gaggggccat cgatccgcgt gcccttcctg
     2281 tacacgatgc tcaccggcac ccgcttgcca tcccgcgcag gtgcccacac ccgctcgctc
     2341 acataatccg atgcatggaa tgtaccgacc acctcctgct gcttgagcag ggtatccttc
     2401 gccgtgtcca tgttgtgctc atacacaccg ttgggtgtgg tcaagctggt gtagccgtag
     2461 cgcagcttgt cgctgtcgaa ctccgggttg acacccgtgt acgccacata ggccgggtca
     2521 ttgaatgaga tcggatgctc ggccccggtg ctgagtttgc gcacgcgcaa gcgggtcaat
     2581 ccgtccttgc gctcactgaa gaccatgtgg tccttgaaca ggtccacatc ctcaaggaga
     2641 acatcctcac gatgcggcac cacttcgacc cacgcctcct tggccgtgct cttcaccgga
     2701 cacttcatgt accggaaatt ggtcgccttc cagttggtca tgatgtagaa ctcaccattc
     2761 gcgtgctgca cactgtactc gtgctcctcc tcacgcggca ggatcacggt gaacttcccg
     2821 ttcggatcgc cgacgggcag gtaccattcc tcggtgctga gcgtgctgcc cgtggagatc
     2881 atcacgaagg cattgctgcg atcgcgatac acatcgcaac tgaaggcatc atccttctcg
     2941 tggaacactt ccacatcgag gcgctcatcg gtgcccagca catgccggaa gatcttgaaa
     3001 ttgcgcagtg tcttgtcctt acggctgtag aagaaggtc
//
LOCUS       DAOQOC010000241         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16907_length_3034_cov_3.334676, whole genome shotgun sequence.
ACCESSION   DAOQOC010000241 DAOQOC010000000
VERSION     DAOQOC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16907_length_3034_cov_3.334676"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PL106_05020"
     CDS             complement(<1..943)
                     /locus_tag="PL106_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA32461.1"
                     /translation="MRWRVFFHGGRQRDLPHLALYWAVQLSAWSLLVFVLALSLWLSN
                     GYTPGRGYGLALGLLIGLVASHTLRAVIRARGWGSAPMAHSLPRMVLAALVISTVAAL
                     FQAVLHDLLFPGSGDFLTARAIFENVINWGILLIVWGVCWFAYQWFMRVRREEVRNLR
                     LEAANRDDQLATLRAQLNPHFMFNALNGIRALIDEDPARAKQAITQLSSILRNAMGTV
                     KRRTVPLGEELDVVKDYLALERMRFEERLQVTFRIEEGIERAPVPPMLLQTLVENAVR
                     HGIAKRKEGGEVIIDARRSGSGLTLGVRNTGTYVPGQV"
     gene            1031..1603
                     /locus_tag="PL106_05025"
     CDS             1031..1603
                     /locus_tag="PL106_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNA32462.1"
                     /translation="MNHLGHLILSGNDPLTITGNFMADAVKGRDLSAHSAAVQRGIRI
                     HRTIDTFTDQHPLTRIGRDRLRAHCGKFAGVALDIFYDHCIALSWKEPEPLPDFAQRM
                     YRVLGTHAHLMPERAQRLLRYLTLRDGLTRYGTLDGIASTLEAMAVRTPAAQALVGAE
                     RVMSEHLDDFVAECSSFLADIRTHLARENG"
     gene            1590..>3034
                     /locus_tag="PL106_05030"
     CDS             1590..>3034
                     /locus_tag="PL106_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNA32463.1"
                     /translation="MRTDKAALAGTLVFASLLALVVGWPLMGLDRATSEDNAHSGPLV
                     ERDLDAIAQDTLRVLVIHDPLTWESRPGAETGLEWELLRRFGRAHALHLRAIPVDDPD
                     SMLMMLQRGEGDVMAGQLSPSGWASPWVAFTKSYRSFAPIRAVLNVDRTMRPAVRETH
                     RPGSPDTLRVSRWSPFIGLEQTFDSSFGAITLLVDPALPEDLLVQVAVGSCPGALVVD
                     AISSVESRRMPQLHFGPRLARSVPLAFAVRRNSPALLAAMNDWLTDPDEVEARDLIAS
                     NYGDGRLTNGPMRSLHSLEFAADSISPFDTLFQAHTDSSNLDWQLLAAVCYKESRFDT
                     SARSSAGAHGLMQLMPKTAAAMGVAPDQGADAHIGGARKYLDRLDSLFLKSVPNKDQR
                     LRFALASYNAGPGHVQDAQRLARLLGLDPRRWDGSVERTLLLLAKPRWYTRSEVQHGY
                     CRAHETFWFVRDVIAAFGQYRDRATRPTSTP"
BASE COUNT          531 a         1055 c          912 g          536 t
ORIGIN      
        1 gcacctggcc aggcacgtag gttcccgtgt tgcgcacacc gagcgtaaga ccgctgcctg
       61 agcgccgggc atcgatgatc acttcgccgc cctccttgcg cttggcgatc ccgtgccgca
      121 ccgcgttctc caccagcgtt tgcagcagca tgggcggtac cggcgcgcgt tcgatcccct
      181 cctctatccg gaacgtcact tggagccttt cctcgaaccg catccgctcc agcgcgaggt
      241 agtccttcac cacatcgagc tcctcgccca agggtaccgt gcgccgcttc accgtgccca
      301 tggcgttgcg caggatggaa ctcaattgcg tgatggcctg tttggcccgc gcgggatcct
      361 catcgatcag cgcacggatc ccgttgagcg cattgaacat gaagtgcggg ttgagctgcg
      421 cgcgcaacgt ggccagctgg tcatcgcgat tggccgcttc gagccgcagg ttgcgcactt
      481 cttcgcgccg cacccgcatg aaccactggt aagcgaacca gcacaccccc cagacaatga
      541 gaagaatacc ccagttgatc acattttcga agatcgcccg agcggtgagg aaatcccctg
      601 atccggggaa gagcagatca tgcaggaccg cctggaacag ggccgccacc gtactgatca
      661 cgagcgcggc cagcaccatc cgcggcaggc tgtgcgccat gggggccgaa ccccagccgc
      721 gcgctcggat cacggcgcgc aacgtgtggc tggccaccag cccgattaag agccccagtg
      781 ccaagccata cccccggcct ggtgtgtacc cgttgctgag ccagagactc agtgcgagca
      841 cgaacaccag cagcgaccat gcgctgagct ggaccgccca gtacagcgcg aggtgcggca
      901 ggtcgcgttg cctgccacca tggaagaaca cccgccaacg catgcggcca agttacccgg
      961 acacacgtgc gcaccgctac cgatgcaacg agcggcgcat ggcccggatg accgggggta
     1021 acttgcgccc gtgaatcacc tgggccatct gatcctctcg ggcaacgacc cgctgacgat
     1081 caccggcaac ttcatggccg acgccgtgaa aggccgcgac ctgagcgccc actcggcagc
     1141 cgtacaacgc ggcatccgga tccaccgcac cattgacacg ttcaccgatc agcatcccct
     1201 cacgcggata ggccgtgatc ggctccgcgc gcattgtggc aagttcgccg gggtggcgct
     1261 cgatattttc tacgaccact gcatcgccct ttcgtggaaa gagccggagc ccttgcccga
     1321 cttcgcacag cgtatgtacc gcgtgctcgg tacacatgcc cacctgatgc ccgagcgggc
     1381 ccaacggctg ctccgctacc tcacacttcg ggacgggctc acacgctacg gcacgctcga
     1441 tggcattgcc agcaccttgg aggccatggc cgtacgcacc ccggccgccc aagcactggt
     1501 gggcgcggaa cgtgtgatga gcgaacacct ggacgacttc gtcgcggagt gctcgtcctt
     1561 tcttgctgac atccgtacac atctcgcacg tgagaacgga taaagccgca ctggcgggca
     1621 ccctcgtgtt cgcgtcgctc ctggccctgg tggtgggttg gccgctcatg ggcctcgacc
     1681 gtgccacgtc cgaggacaac gcccactcgg gccccttggt ggaacgggac ctggatgcca
     1741 ttgcacagga caccctgcgc gtgctggtga tacacgaccc cttgacctgg gagagccgcc
     1801 ccggtgcgga aaccggactg gagtgggagc tgctccgtcg tttcggccgc gcacacgccc
     1861 tccacttgag ggccattccg gtggatgatc ccgacagcat gctcatgatg ctgcagcgcg
     1921 gcgaaggcga tgtgatggcc ggacaactga gtccttcggg ctgggcttca ccttgggtgg
     1981 ccttcacgaa aagctaccgc tccttcgctc cgatccgggc cgtgctcaac gtggaccgta
     2041 ccatgcgccc ggccgtgcgc gaaacgcacc ggcctggatc gcctgacacc ctgcgcgtga
     2101 gccgctggtc gccctttatt gggctggaac aaacgttcga cagttcgttc ggtgccatca
     2161 cgctgctggt ggatccggcc ctgcccgagg atctgctggt acaggtcgcg gtgggcagct
     2221 gtccgggggc tctggtcgtg gacgcgatct cttcggtgga atcccgtcgg atgccgcaac
     2281 tccatttcgg cccacggctc gcgcggtcgg tgcccctggc cttcgccgtg cgcaggaaca
     2341 gcccggctct cctggccgcc atgaacgact ggctcaccga tcccgacgaa gtggaagcgc
     2401 gcgacttgat cgcttcgaac tacggtgatg gccgcctgac caacgggccg atgcgcagtc
     2461 tgcacagcct ggaattcgcc gccgacagca tctccccctt cgacacgctt ttccaagccc
     2521 ataccgacag ttccaacctc gactggcaac tgctcgcggc ggtttgttac aaggaatcgc
     2581 gattcgatac cagtgcccga tccagcgcag gcgcccacgg attgatgcag ttgatgccca
     2641 agaccgcggc cgccatgggt gtggcccccg accagggtgc cgatgcccat atcggcggag
     2701 cccgcaagta ccttgaccgg ctggattccc tgttcctgaa aagtgtgccc aacaaggacc
     2761 agcggcttcg ctttgctctg gcctcgtaca acgccgggcc tggccacgtt caggacgccc
     2821 agcgcctggc ccgactgctc ggcctggatc cccgacgctg ggacggttcg gtggaacgca
     2881 ccttgctgct actggccaag cctcgctggt acacgcgcag tgaagtgcag catggctact
     2941 gtcgcgctca tgaaacattc tggttcgtgc gtgatgtgat cgccgctttc ggtcagtacc
     3001 gcgaccgcgc cacccgcccg acctccacgc catt
//
LOCUS       DAOQOC010000242         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_16991_length_3026_cov_2.935712, whole genome shotgun sequence.
ACCESSION   DAOQOC010000242 DAOQOC010000000
VERSION     DAOQOC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16991_length_3026_cov_2.935712"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PL106_05035"
     CDS             complement(<1..323)
                     /locus_tag="PL106_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32464.1"
                     /translation="MIDRTLTASAIALTGLVACGPTPVATTEVRPVPAVDSSAVRPFV
                     LGETRTVRSTILDQDRVINVSLPAGYSPDSATVYPVIYLLDGSADEDFVHVAGLVGFM
                     STYAL"
     gene            412..1446
                     /gene="mnmH"
                     /locus_tag="PL106_05040"
     CDS             412..1446
                     /gene="mnmH"
                     /locus_tag="PL106_05040"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNA32465.1"
                     /translation="MIRPLTIHEWLLESTPIVDVRSPGEFARGHIPGAVNVPLFDDDE
                     RAVVGTLYKRAGRDAAMLEGLRITGPKLADLVEQARETAPDGHIRIHCWRGGERSGSM
                     AWLLDKAGFARAHTLIGGYKAFRHEVINSFNTPVDLHVLGGYTGSGKTETLGQLRVLG
                     QQVVDLEALACHKGSAFGALGEVPQPTTEHFENLLWNALRDLEPQRPIWVEDESLMIG
                     RVRIPDAFFHQMRQARLFFAEMPVEQRVERLVVDYGRFAKEDLAASVRRIEKRLGPQH
                     CKAALEALDRNDLHSVARIALVWYDKAYLRGTAERDPQRVVRLPADANDLRGLAQRLK
                     EDAAAHRTGN"
     gene            1418..2488
                     /gene="selD"
                     /locus_tag="PL106_05045"
     CDS             1418..2488
                     /gene="selD"
                     /locus_tag="PL106_05045"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNA32466.1"
                     /translation="MPQPTAPGTEPIRLTSLSPAAGCGCKIAPSELEKILHGQEAIIP
                     DPRLLVGYGSRDDAAVYDLGGDRCLISTTDFFAPIVDDPFDFGRIAATNALSDVYAMG
                     GTPLLAVAILGWPIEKLGAGPARMVVEGGRRACHDAGIVLAGGHSIDAPVPFFGLAVT
                     GEVNRACLKRNDTAHAGDRLYLTKPLGIGILSTALKRGQLPESEKMIGRDLMAGLNSV
                     GIALGKLDTVHALTDVTGFGLLGHLLEVCRGSGLNAALRYDAVPKIPEALRCLAGGHY
                     PDGALRNWKSYAHEVSGASTMERMLLLSDPQTSGGLLIACAPEGAADLERTLRDAGIE
                     AGPIGQLHARDAQVQGARVVVE"
     gene            2574..>3026
                     /locus_tag="PL106_05050"
     CDS             2574..>3026
                     /locus_tag="PL106_05050"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA32467.1"
                     /translation="MRALFGLILLLTGFSISAQDATISGTVRDESGAVMPGVSVSVRG
                     PKPVGSTTDDKGRYELRIPPEDLEDQTVTVRFAFLRELVQRDVAILPGEHRDLDIAMS
                     IKSLPDVEVTKEGLRDRSAIDKLDARVTRFMPNPLGDVNSLLQGRLGVV"
BASE COUNT          564 a          940 c         1010 g          512 t
ORIGIN      
        1 atcaacgcgt aagtactcat gaaaccgacc agccccgcca catgcacgaa gtcctcgtcg
       61 gccgaaccgt ccaggagata gatcacgggg tacacggtcg cactgtccgg agaatagccc
      121 gccggtagcg aaacattgat aacccgatcc tgatccagaa tggttgaacg cacggtgcgg
      181 gtttcgccga gaacgaaggg gcgtaccgcg ctgctgtcca cggcgggcac cgggcggact
      241 tccgtggttg ctacgggtgt cggcccgcaa gccaccagac ctgttaacgc gatggcagag
      301 gccgtgaggg tgcggtcgat catggtggaa cagatgaaca aagatgagcg ctattccgct
      361 ttcacgcgta ccgaaagctc tggcacccga ccttggccct gatccagccg catgatccgc
      421 cccttgacca tacacgaatg gttgctggaa agcacgccca tcgtcgacgt gcgctcgccc
      481 ggggagttcg cacgcgggca tatccccggc gcggtgaacg taccgttgtt cgatgatgat
      541 gaacgggcgg tcgtgggcac cttgtacaag cgtgcgggcc gtgatgccgc catgctggag
      601 ggtctgcgga tcacggggcc gaagctggcc gacctggtgg aacaggcccg ggaaacggca
      661 ccggacggcc atattcgcat ccactgctgg cggggcgggg agcgcagtgg cagcatggcg
      721 tggctgctgg acaaggccgg cttcgcccgg gcacacacct tgatcggcgg gtacaaagcc
      781 ttccgccatg aggtgatcaa ttcgttcaac acccccgtgg acctgcacgt gctgggcggc
      841 tacaccggct cgggcaaaac ggaaaccctg ggccaattgc gtgtgctggg gcaacaggtg
      901 gtcgacctgg aagccttggc ctgccacaag ggatcggcct tcggggcact cggcgaggta
      961 ccccagccca ccaccgagca tttcgagaac ctgctctgga acgcgctacg cgatctggag
     1021 ccgcagcggc ccatctgggt ggaggatgag agcctgatga tcggtcgggt gcgcataccg
     1081 gacgcattct tccatcagat gcgacaggcc cggctcttct tcgccgagat gccggtggaa
     1141 caacgggtgg aacggctggt ggtcgattat ggccgcttcg cgaaggaaga cctggccgca
     1201 tccgtccgac gcattgaaaa gcgcctcggg ccgcagcact gcaaggccgc cttggaggca
     1261 ctggatcgca acgacctgca cagcgtggcc cggatcgccc tggtgtggta cgacaaggct
     1321 tacctgcgcg gcaccgccga gcgtgatccg cagcgcgttg tgcgactacc ggccgatgcg
     1381 aacgatcttc gcggcctggc acaacgcctg aaggaagatg ccgcagccca ccgcaccggg
     1441 aactgaaccg atccgcctca ccagcttgag tcccgctgct ggctgcggct gcaagatcgc
     1501 accgtccgaa ctggaaaaga tccttcacgg gcaggaggcg atcatacccg acccgcgatt
     1561 gctggtgggc tatggctcac gcgatgatgc cgccgtttac gacctgggag gagaccgctg
     1621 cctgatcagt accaccgact tcttcgcccc gatcgtggat gaccccttcg acttcggacg
     1681 catcgctgcc accaacgcgc tgagcgacgt gtacgccatg ggaggaacgc ccttgctggc
     1741 ggtggccatc ctgggctggc ccatcgagaa actcggggcc ggacccgcac ggatggtggt
     1801 tgagggcggc cgacgtgcat gccatgatgc cggtatcgtt ctggcgggtg ggcacagcat
     1861 cgatgccccc gttcccttct tcgggctggc cgtcacgggc gaggtgaacc gggcctgcct
     1921 caaacggaac gatacggcgc atgcaggcga tcggctctac ctgaccaagc cgttgggcat
     1981 cggcatcctg agcaccgccc tcaagcgggg ccagttgccc gagtctgaaa aaatgatcgg
     2041 ccgggacctg atggcaggcc tgaacagcgt gggtatcgcg ctcggcaaat tggacacggt
     2101 gcatgccctc accgacgtca cgggcttcgg actgctgggg cacctcttgg aagtgtgccg
     2161 gggcagtgga ctgaacgcag cactgcgata cgatgccgtg ccgaagatcc ccgaggcgtt
     2221 gcgttgcctg gccgggggac attatccgga cggcgccctc cgcaattgga aaagctacgc
     2281 gcacgaggtg agtggtgcga gcacgatgga gcgcatgctg ctgctgagcg atccgcagac
     2341 cagcggtggc ctgctcatcg cctgcgcgcc cgaaggtgcg gccgatttgg agcgcacgct
     2401 gcgcgacgcg ggcatcgaag ccgggcccat cgggcagctc cacgcacgtg atgcgcaagt
     2461 gcagggcgcg cgcgtggtgg tcgaataggg cctggtccgc acacaaggca ctaatagcgg
     2521 gaacggcccg ttatattggc cgcccgccga agttgcaggc gaattgatcc ttgatgcgcg
     2581 ccctcttcgg tctgatcctt ctgcttaccg gtttttcgat ctctgctcag gacgccacga
     2641 tctcgggcac cgtgcgcgat gagagcgggg ccgtgatgcc gggcgtatcc gtgagcgtgc
     2701 gcggtccgaa gcctgtgggc tccactaccg acgacaaggg gcggtacgag ttgcgtattc
     2761 caccagagga cctggaggac cagaccgtaa cggtgcgctt cgccttcctc cgggagctgg
     2821 tgcaacgcga tgtggccatc ctgccgggag agcaccggga tctggacatc gccatgagca
     2881 tcaagtcctt gcccgatgtg gaagtgacca aggagggcct gcgcgaccgc agtgcgatcg
     2941 acaaactgga cgcgcgggtc acccgcttca tgcccaaccc attgggcgat gtgaacagcc
     3001 tgctgcaggg gcgccttggt gtggtc
//
LOCUS       DAOQOC010000243         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17000_length_3025_cov_3.917172, whole genome shotgun sequence.
ACCESSION   DAOQOC010000243 DAOQOC010000000
VERSION     DAOQOC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17000_length_3025_cov_3.917172"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PL106_05055"
     CDS             complement(<1..198)
                     /locus_tag="PL106_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32468.1"
                     /translation="MSFDIKRGQGISIDQLLDAVDSLSMDECQRLAGRLEKRGIALRF
                     KLLLSKLKDAKLDERTIRRTTE"
     gene            complement(218..1180)
                     /gene="fbp"
                     /locus_tag="PL106_05060"
     CDS             complement(218..1180)
                     /gene="fbp"
                     /locus_tag="PL106_05060"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNA32469.1"
                     /translation="MSQVKTLAEFIVERQHEFTYATGELSQLLTSFRLAGKVVNREVN
                     KAGLAEDILGAQGTENVQGEAQQKLDVYANDLFIRLLRSQGMVCAVASEENDDIVHFD
                     NGGKYVVTMDPLDGSSNIDVNVSIGTIFSIYRRVSTGDKATMEDFMQPGTAQVAAGYI
                     IYGSSTMLVYTTGHGVNGFTLDPSIGTFCLSHPDMKTPADGKIYSVNEGNLYDFSPGV
                     QQYIDGCKKQKMSARYIGSLVADFHRNLMKGGIYMYPGTAKAPKGKLRLLYEANPLAF
                     IVEQAGGKATDGTVRIMELKPTELHQRCPLFIGSKAMVEAATKA"
     gene            1255..2298
                     /locus_tag="PL106_05065"
     CDS             1255..2298
                     /locus_tag="PL106_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNA32470.1"
                     /translation="MTDELTPLVGRIADIIQQARSRVRQSVNSAMVASYWEIGRLIVE
                     HEQQGQARAEYGKRQLAELSERLTEQFGKGFDASNLRNMRQFHLAFPIRDALRHELSW
                     THYRTLLRVEGQAAREWYAREAISQNWSTRALERQIGVLYYERLLSSKDKALVATEAT
                     ERIAPLAESPKDFLRDPYILDFLNLQDKTYQESTLEQAIIDNIQSFLLELGKGFAFVE
                     RQQRIRIGDEDFYLDLVFYNFKLKCFLLLDLKLGKLGHQDVGQMDTYVRLYDERIRGA
                     DDNPTIGLLLCSEKSEAVVKYSVLADQQQLFAAKYLPYLPSVEDLKRELERERERAMR
                     QIEAGEGNKDKAP"
     gene            2383..2937
                     /locus_tag="PL106_05070"
     CDS             2383..2937
                     /locus_tag="PL106_05070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA32471.1"
                     /translation="MQVTIRPAEPRDVPRMLELVRELALFERAPDEVTVTEEHMRDAG
                     FGKDPVWFGWVAEGEVVRSGLSVVSTASGGAPNNQQLSPDNCILGMAICYTRYSTWKG
                     RRLYLEDIIVTETARGHGIGDKLFRQCARYAVEQNYSGMLWQVLDWNTDAIRFYERFG
                     AEFSGEWLNGSLGKERLAELAATS"
BASE COUNT          577 a          918 c          910 g          620 t
ORIGIN      
        1 ctctgtggtg cgccggatgg tgcgctcgtc gagcttcgcg tctttcaatt tggacagcag
       61 caacttgaag cgcaaggcta ttccgcgttt ctccaagcgg cccgccaagc gctggcactc
      121 atccatcgac aaggagtcca ctgcatccag caactgatcg atggatatcc cttgtccgcg
      181 ttttatatcg aaggacatgc tgctcgttcg tccaaagtta cgccttcgtc gccgcctcca
      241 ccatcgcctt gcttccgatg aacaacgggc agcgctggtg cagctcggtg ggcttcaact
      301 ccatgatgcg taccgtgcca tcggtggcct tgccccccgc ctgttccacg atgaaggcga
      361 gcgggttcgc ctcgtacagc aggcgcagct tccccttggg agccttggcg gtaccgggat
      421 acatgtagat ccctcctttc atcaggttcc ggtggaagtc cgccaccagg ctgccgatgt
      481 agcgcgcgct catcttctgc ttcttgcagc cgtcgatgta ctgctgcacg ccggggctga
      541 agtcgtagag gttgccttcg ttcaccgagt agatcttccc gtccgccggc gtcttcatgt
      601 ccggatgact caggcagaag gtgccgatgc tcggatccag cgtgaagccg ttcaccccgt
      661 ggcccgtggt gtacaccagc atggtactgc taccgtagat gatgtatcca gccgccacct
      721 gcgcggtgcc cggttgcatg aaatcctcca tcgtggcctt gtcgccggtg ctcacccggc
      781 ggtaaatgct gaagatggtg ccgatgctca cgttcacgtc gatgttgctg cttccgtcca
      841 gcgggtccat cgtcaccacg tacttgccgc cgttgtcgaa gtgtacgatg tcgtcgttct
      901 cctcactggc cactgcgcac accatgccct ggctccgcag cagacggatg aagaggtcgt
      961 tggcgtacac atcgagcttc tgctgggcct cgccctgcac gttctcggtg ccctgggcac
     1021 ccaggatgtc ctcggccagg ccggccttgt tcacctcccg gttcaccacc ttgcccgcga
     1081 ggcggaagct ggtgagcaac tggctgagtt cgccggtggc gtaggtgaat tcgtgttggc
     1141 gttcaacgat gaactcggcg agggtcttga cctgtgacat gtatttgcga tgtgcgctgc
     1201 aaagatgcgg cagggcttcc tttgcatcat gtacctgaca aaccgccccg ccggatgacc
     1261 gatgaactga ccccgcttgt cggacggata gccgacatca ttcagcaggc ccgcagccga
     1321 gtgcgccaaa gcgtgaacag cgccatggtg gccagctact gggagatcgg ccgcttgatc
     1381 gtggagcatg agcaacaagg ccaggcccgc gcggagtacg gaaagcgtca attggccgag
     1441 ctttccgagc ggctcaccga gcagttcggc aaagggttcg atgccagtaa tctgcgcaat
     1501 atgaggcagt tccacttggc tttcccaatt cgcgacgcac tgcgtcacga attgagctgg
     1561 acccactacc gcaccttgtt gcgcgttgag ggacaggccg cccgtgagtg gtacgcccgt
     1621 gaggccatca gccagaactg gagtactcgc gccctggagc gccagatcgg cgtactctat
     1681 tacgaacgcc tgctcagtag caaggataag gcgctggtgg caaccgaagc aaccgaacgc
     1741 attgcccctt tggcagagtc tccgaaggac ttcctgcgcg acccgtacat cctggatttc
     1801 ctgaacctgc aggacaagac ctaccaggaa agcacgctgg agcaggccat cattgacaac
     1861 atccaaagct tcctgctgga actgggcaag ggcttcgcct tcgtggaacg gcaacagcgc
     1921 atccgcatcg gggacgagga tttctacttg gacctggtgt tctacaattt caagctcaag
     1981 tgtttcctgt tgctggacct gaagctgggc aagttgggcc accaggatgt gggtcagatg
     2041 gacacgtatg tgcgtttgta tgacgagcgc atccgtggtg cggacgataa tcctaccatt
     2101 ggcctgttgc tgtgcagcga gaagagcgag gctgtggtga agtattccgt gctggccgac
     2161 caacaacaat tgttcgccgc gaagtacctg ccgtacctgc ccagcgtgga ggatctaaag
     2221 cgcgaattgg aacgtgaacg cgaacgggcc atgcggcaga tcgaggcggg ggagggcaac
     2281 aaagacaagg ccccatgaac cgcgtgtacc cgcgcttccg ccggttgcaa cgccccattg
     2341 atcaacaagc ctgagacctt cacccaacct cgataatccg ccatgcaagt gaccatccgc
     2401 cccgctgaac cccgcgacgt gccccgcatg ctggagctgg tacgggagct ggcgctgttc
     2461 gagcgcgccc ccgatgaagt gacggtgacc gaggaacaca tgcgcgatgc gggcttcgga
     2521 aaggatccgg tgtggttcgg ctgggtggcc gagggggaag ttgttcgttc cgggttgtca
     2581 gttgttagta cggcctccgg aggtgcccct aacaaccaac aactatcacc ggacaactgc
     2641 atcctcggca tggccatttg ctacacccgc tactccacct ggaaagggcg tcggctctac
     2701 ctggaggaca tcatcgtaac cgaaaccgcg cgcggccatg gcatcggcga caagctcttc
     2761 cgccaatgcg cgcgctacgc cgtggagcag aactacagcg gcatgctctg gcaggtgctg
     2821 gactggaaca ccgacgcgat ccggttctat gagcggttcg gggcggagtt ctcgggggag
     2881 tggctgaacg gttcactggg gaaggagcgg ttggccgagc tggcggccac atcatgagcg
     2941 atcaacctcc ggggcgcact tcctgggcca ctttcgcccg tgccttcaac acctgatgca
     3001 cggcatggtc cttctggaat tgtgc
//
LOCUS       DAOQOC010000244         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17014_length_3024_cov_3.434490, whole genome shotgun sequence.
ACCESSION   DAOQOC010000244 DAOQOC010000000
VERSION     DAOQOC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17014_length_3024_cov_3.434490"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3024)
                     /locus_tag="PL106_05075"
     CDS             complement(<1..>3024)
                     /locus_tag="PL106_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HNA32472.1"
                     /translation="DGLGDVCDNCPNDANGAQDDGDNDNVGDACDACPTNGNLPVGPN
                     GCPTSIYVNDGNTGDDVVTTAAGNDATGDGTSAAPFATIMHAMGLVPDGGTIIVDGGT
                     YTEQVVTGGKNVTITGQGRSGATMTRILAPATLTNYVTNGGDHYPIVYASGLGKTFNL
                     NNVLVDGDNGRDLDYYGIYFYEASGSVNFCRVTDIHDAGVVNGMQRGVGIMANHIFDA
                     SVTQSVTIDNNIIDNYQKGGVVLNELNTEGVVTNNQVTWSGGPNVNGANGIQFGWGSW
                     GTITGNTVAGNVWNEPAPHTWVAGGIILVGVGVDYTNTPTGTTTTVSGNTLNGNESGI
                     LVDEGGYGYDSNAGLVEGPNTYSGNWINVWLEAPATVPNGSNTYDKRVDNASLTNVVY
                     GSIQYAVDFGSTGDVLTASAGTFTENVTVDKTNMSLNGAQAGNARCTPATESTIDGGT
                     GTAVTIAADGVTLNGFHLDAKTGVSSSTWSGATISYNLVDADLYGIAAQGMATGHTVN
                     ANCVDMTQQLDGPTPTAGIALVGSSTAPTVTNNTVKDGYYGYVLYAMPATTTVSGGSV
                     SGTQLGVAISNSTATAAPWGLLSPSNVTVSGVSMSGFTGAAGAHAGVYTFTAPGTLPV
                     QGLNVSVTGCTIDGTGKPAANSAAIGFEDYSGGGVTVQNVSVANSTLTNNSNRGIHVR
                     GRVVADIDGSVLTNNGYDAFGSGGNVGFGVLVSEDASATVNNCIINNPTSATPYAVHA
                     LALGNGSTNSLVATDNIIGVHYAEANSFGATNNGTGTLTAECNYWAETAYQDILPRIN
                     GTVDFINWRQDGTDDGDPSNGFQPQSPADDCTGNPITDFAAAFGSDATCELTADGTAT
                     SSVSTGVGPYTYLWNRTLPVSPAHTSTMEDPTDLLPGTYDVTVTDALGSTATVSGVVI
                     GITPHSGTDWYVSTTGSNTNTGMAGCPFATIQFAINASSNGHTVHVANGTYDENVALN
                     KDIDLIGQGVSTIVQPSTPCNGTGVSITADGASIS"
BASE COUNT          505 a          934 c          907 g          678 t
ORIGIN      
        1 aactgatgct cgcaccatcg gcggtgatcg acacacccgt gccgttgcac ggggtggagg
       61 gttggacgat ggtcgaaacg ccctgcccga tgaggtcaat gtccttgttc agagcgacgt
      121 tctcatcgta cgtgccattc gccacatgca ccgtatggcc gttgctcgac gcgttgatcg
      181 cgaactggat cgtggcgaag ggacagccag ccatgcccgt attcgtattg ctgcccgtgg
      241 tgctcacgta ccagtccgtg ccactgtgcg gtgtgatgcc gatcaccacg ccgctgaccg
      301 ttgccgtgga gcccaacgca tcggtcacgg tcacgtcgta ggtgcccggc aacagatcgg
      361 tgggatcctc catggtggag gtatgtgcgg gagaaaccgg taaggtgcgg ttccacaggt
      421 acgtgtatgg gccaacacct gtactcaccg aggaggtcgc agttccgtcc gcggtcaact
      481 cgcaggtggc gtcgctgccg aaagcagcgg cgaagtccgt gatcgggttg cccgtgcaat
      541 catccgccgg gctctgcggc tggaagccgt tgctcgggtc tccgtcatcc gtgccgtcct
      601 ggcgccagtt gatgaaatcc accgtgccgt tgatccgggg gagaatgtcc tggtaggctg
      661 tttccgccca gtagttgcac tcggcggtga gcgtacccgt gccgttgttg gtggcaccaa
      721 agctgttggc ctccgcgtag tgtacgccga tgatgttgtc cgtggccacc aggctgttcg
      781 tgctgccgtt gcctaaggcc agtgcatgca cggcgtaggg ggtggcactg gtggggttgt
      841 tgattatgca gttgttcacc gtggcgctgg catcctccga gaccagcaca ccgaaaccaa
      901 cgttcccacc ggaaccgaag gcatcgtacc cattgttggt gagcacggat ccgtcgatat
      961 cggccaccac tcggccccgc acatggatcc cacggttgct gttgttcgtc agtgtgctgt
     1021 tggctacact cacgttctgt acagtcactc cgccgccgct gtaatcctcg aagccgatgg
     1081 ccgcactgtt ggcggcaggc ttgccagtgc cgtcgatcgt gcagccggtc acactcacat
     1141 tcagcccttg taccggtaag gtgccgggtg ccgtgaaggt gtataccccg gcgtgcgcgc
     1201 ctgccgcacc cgtgaacccg ctcatgctca cgccgctcac ggtcacgttc gagggcgaca
     1261 agaggcccca cggtgccgca gtggcggtgg agttgctgat ggccacgcca agctgagtgc
     1321 cgctcaccga gccaccgctc acggtggtgg tcgccggcat ggcgtacaac acgtatccgt
     1381 agtagccgtc cttcacggtg ttgttcgtta ccgtgggcgc ggtgctggaa ccgaccagcg
     1441 cgatgcccgc agtgggggtg ggtccatcga gctgctgggt catatccacg cagttggcgt
     1501 tcaccgtgtg gccggttgcc atgccctgtg cggcaatgcc gtacagatcg gcatccacca
     1561 ggttgtagct gatcgtggca cccgaccaag tggagctgct caccccggtc ttggcgtcca
     1621 agtggaaacc gtttagggtg accccatccg ccgcgatcgt aacggccgta ccggtgcctc
     1681 cgtcaatcgt cgattccgtt gcgggcgtgc aacgcgcgtt cccggcttgc gccccattta
     1741 gggacatgtt cgtcttgtcc accgttacgt tctcggtgaa ggtgcccgcg cttgcggtga
     1801 ggacatcccc agtggaaccg aaatcgaccg cgtactggat ggatccgtac accacattgg
     1861 tgagggaggc attgtccact cgcttgtcat aggtattgct gccgttgggg accgttgcag
     1921 gcgcctccaa ccagacattg atccagttgc cactatacgt gttcggccct tctacaaggc
     1981 ccgcattgct gtcgtacccg tagccccctt catcgacgag gatgcccgac tcattcccat
     2041 tgagtgtgtt tccgcttacc gtggtggtcg ttcctgtcgg ggtgttcgta tagtccacac
     2101 ccacacctac aagaatgatc cctcctgcga cccaggtatg cggtgccggt tcgttccaaa
     2161 cgttgcctgc caccgtgttg cctgtgatgg taccccagga cccccagccg aactggatgc
     2221 cgttggcccc gttcacattg gggcctccgc tccaagtgac ctggttgttg gtgactacgc
     2281 cctccgtgtt cagttcgttg agcaccacac cgcctttttg ataattgtcg atgatgttgt
     2341 tgtctatcgt gacactttgg gtcaccgatg catcgaagat gtggttcgcc atgatgccga
     2401 cgccgcgctg catgccgttc actactcccg catcatggat gtccgtcacg cggcagaagt
     2461 tcacggagcc gctggcctcg taaaagtaga tgccgtaata gtccaggtcc ctgccgttat
     2521 caccatccac cagcacattg ttcagattga aggtcttgcc cagacccgaa gcgtacacga
     2581 tcgggtaatg gtcgccaccg ttggtgacat agttggttag cgtggcagga gccaggatcc
     2641 gggtcatcgt cgctccgctg cggccctgtc cggtgatggt gacgttcttg cctcctgtaa
     2701 caacttgttc ggtatacgta ccgccgtcca cgatgatggt tccgccatcc ggaaccagac
     2761 ccatggcatg catgatcgtg gcgaagggtg ccgccgaagt accatcgccg gtcgcatcgt
     2821 taccggccgc tgtggtcacc acatcatccc ccgtgttccc atcgttcacg taaatggatg
     2881 tcgggcatcc gttcggtcct accggcaggt tgccattggt cgggcaggca tcacaggcgt
     2941 ctcccacatt atcgttgtca ccgtcgtcct gggcaccgtt cgcatcgttg gggcagttgt
     3001 cgcacacatc acccagtcca tcgg
//
LOCUS       DAOQOC010000245         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17029_length_3023_cov_2.904650, whole genome shotgun sequence.
ACCESSION   DAOQOC010000245 DAOQOC010000000
VERSION     DAOQOC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17029_length_3023_cov_2.904650"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PL106_05080"
     CDS             complement(<1..333)
                     /locus_tag="PL106_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA32473.1"
                     /translation="MIRLTGIRKSYRTGSSTLEVLKGIDLHIAKGELVSIMGASGSGK
                     STLLNIIGILDNYDSGEYRLDSLLIKGQSETENAGLRSKYIGFIFQSFNLISFKNALE
                     NVALPLYYQ"
     gene            complement(447..1046)
                     /locus_tag="PL106_05085"
     CDS             complement(447..1046)
                     /locus_tag="PL106_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32474.1"
                     /translation="MRCPHLRFAGVCCVWGASLLSPAQRSTFDVIRGGEKVGSISTSR
                     TTFGDRTLYVTLSWAEFDLLWRQNVRTVNTTEYAADTVLTCRTAVHVNGSIRDSSHMA
                     PTSDGLRCYVYPGRVFRGTPRTDWTTARMYFEEPVGQDLVFVESVLEPCSLLSTAPGR
                     YMLIFPNGARNRYRYANGALQEVQVDRSFLDLVFRRRPD"
     gene            complement(1179..2936)
                     /locus_tag="PL106_05090"
     CDS             complement(1179..2936)
                     /locus_tag="PL106_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32475.1"
                     /translation="MATTDLTRALVSLAIGWATLHTAAGQCGTTIYDTGGSGGNYGNS
                     QNLTWTYCAPPGQYLTITFTYFDTELDYDWLSIHDGATNAAPMLGEWSGTGNIGSYTT
                     STPGGCLTLWFTSDFIFTYGGWAATITCTSPPAGDCVYQLNMFDTFGDGWDGSTVGIS
                     INGGPFSNYTITGAQGSAMIGVNIGAVVVVQFTNNSPFPDEMSYSLTLGGGVLFSSGS
                     PPATGIVFTNTVTCDPPPAAPQDCLGATTICSGQQFNNNSNNTGAVMDLNASNQGCLS
                     LGERQGTWYVFSPSASGTIGFSLTPTGLTDYDFAIWGPYPDGSTPSTICPPAGPPIRC
                     SYDAPGPYTTGLATGAGDNSEDALGDGWVNSIPVTVGQVYIMYIDNWSNDGQSFSLSW
                     QLTNGASLDCTVLPIELVNFNAQVVDEGVALSWTTATEQNSDHFEVERSFDGEHFVTI
                     GILNAAGESQQQNNYSFLDRQPQYGLNHYRLRLVDTDGDTDGSGVRIVQVDRGGMEEV
                     LLVPNPGTDNLQVLLASTVVDGSTFLLVDATGRTVSRFRTQNTRMEINTAVLPKGLYG
                     FRLLAPDGSIVAHGIWVRE"
BASE COUNT          601 a          832 c         1012 g          578 t
ORIGIN      
        1 ctgatagtac aagggcagcg ccacattctc caaggcgttc ttgaaggaga tgaggttgaa
       61 gctctggaag atgaaaccga tgtacttcga acggagcccg gcgttctccg tttcactctg
      121 gcctttgatg agcaggctgt ccaaccggta ttcgccgcta tcgtaattgt ccaggatgcc
      181 gatgatgttc agcagggtgc tcttgcctga gccggaagcg cccatgatgc tcaccagttc
      241 gcccttggcg atgtgcaggt cgatgccctt gagcacttcg agcgtgctgc taccggtacg
      301 atagctcttg cggatgccgg tgaggcggat catgtggcgg tgaaagtacc tgtcgtggac
      361 cgagcggtcg ggggccttac acgaacggtg cgcatgcggg gaggagcgga gagggatcgg
      421 gcaggcctcc gggccctgtc aagtgtttaa tccggccgcc gccggaacac cagatccagg
      481 aaagaccggt ccacctgcac ctcctgcaac gcgccgttgg catatcggta acggttccgg
      541 gcaccgttcg ggaagatgag catgtatcgt cctggggccg tggacaacag ggagcaaggc
      601 tccagtacgc tttccacgaa gacgagatcc tggcccacgg gctcttcgaa gtacatgcgt
      661 gcggtggtcc agtccgtacg cggcgtgcca cggaataccc tccccggata cacgtagcag
      721 cgaagtccgt cgcttgtcgg ggccatgtgg ctggagtcgc ggatggaccc gttcacatgg
      781 acggcggtgc ggcaggtgag caccgtatcg gccgcatact cggtggtgtt cacggtgcgc
      841 acgttctgtc gccacaagag atcgaattcg gcccaggaga gcgtgacata cagggtccga
      901 tcgccgaacg tggtgcgcga ggtgctgatg ctgcccacct tttcacctcc ccggatcacg
      961 tcgaaggtgg atcgctgagc agggctcagc agcgatgctc cccatacaca gcaaacaccg
     1021 gcgaagcgga ggtggggaca gcgcatcgtg ggggaaaata ggacgatcag gaacggttgt
     1081 gcacatccac ggctcacaat ggggacatcg ggatcacgca gtggagcaga aagggcatga
     1141 ccggaaaggc taccgggaac gggaatacgg agcctggatc attcgcgcac ccagatgccg
     1201 tgcgccacta tggagccatc aggggcgagc aggcggaaac catacaaccc cttcggaagg
     1261 acagcggtgt ttatctccat acgcgtgttc tgcgtgcgga accgtgagac cgtgcgtccg
     1321 gtcgcatcca ccaataggaa tgtggatcca tcaacaacgg tcgaggccag cagcacttga
     1381 aggttgtccg tacccgggtt gggtaccagc aacacctcct ccatgccgcc ccggtccact
     1441 tgtacgatgc gcacacccga tccgtccgta tcgccatccg tgtccaccaa gcgtaggcgg
     1501 tagtggttca agccatactg tggctggcgg tccaggaagg agtaattgtt ctgttgttgg
     1561 ctttcgcccg ccgcgttcag gatgccgatg gtcacgaaat gttcgccatc gaacgagcgc
     1621 tccacctcga aatggtcgct gttctgctcg gtggcggtgg tccacgacag ggccacaccc
     1681 tcgtcaacaa cttgggcgtt gaagttcacc aactcgatgg gaaggacggt gcaatccaac
     1741 gaggccccgt tggtgagctg ccagctgagc gagaacgact gaccatcgtt ggaccagttg
     1801 tcgatgtaca tgatgtacac ttggccaacg gtcacgggta tcgagttgac ccagccgtcg
     1861 cccaaggcat cttcggagtt gtcgccggcc ccggtggcca agccggtggt gtacggcccg
     1921 ggggcatcat aagagcaccg gataggtggt ccggcgggtg ggcagatcgt ggagggtgtg
     1981 gatccatccg ggtagggccc ccagatggcg aagtcgtaat cggtgagccc ggtgggtgta
     2041 agggagaagc cgatggtacc gctggccgaa ggggaaaaaa cataccaggt cccttgtcgc
     2101 tcgcccaggg aaagacaacc ctgattcgac gcattgaggt ccatcaccgc tccggtgttg
     2161 ttggagttgt tgttgaactg ctggccgcta cagatggtgg tggcgcccag gcaatcctgc
     2221 ggcgccgcag gtggcggatc gcaagtgacc gtattggtga acacgatccc ggtggcgggc
     2281 ggggatcccg agctgaacag gacaccaccg cccaaggtga gcgaatagga catctcgtcc
     2341 gggaacgggc tgttgttggt gaactggacc acgaccacag cgccgatgtt cacaccgatc
     2401 atggccgagc cttgcgcgcc ggtgatggtg tagttggaga atgggccgcc gttgatgctg
     2461 atgcccacgg tggacccgtc ccagccatcg ccgaaggtgt cgaacatgtt caattgatag
     2521 acgcaatcgc ctgccggagg agaggtgcag gtgatcgttg ccgcccagcc accgtacgtg
     2581 aagatgaaat cggaggtgaa ccagagggtg aggcagccac cgggtgtgga ggtggtgtag
     2641 gagccgatgt tcccggtgcc gctccattcg ccgagcatgg gtgcggcatt ggtggcacca
     2701 tcgtggatgg agagccaatc gtaatccagt tcggtatcga agtaggtgaa ggtgatcgtg
     2761 aggtactggc cgggcggggc acaataggtc caagtgaggt tctggctatt gccgtagttc
     2821 ccacctgatc cgccggtatc ataaatggtg gtgccgcatt gtcctgctgc ggtatgcaga
     2881 gtggcccagc cgatcgccag ggacaccagt gcccgggtca ggtcagtggt cgccatgggc
     2941 aggtcgggct tgatccatca gcgcttggcg atcggcgcgg gtggtggtga gggtggagtg
     3001 tcgttggcct gtaccgcttc ctc
//
LOCUS       DAOQOC010000246         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17053_length_3021_cov_3.281861, whole genome shotgun sequence.
ACCESSION   DAOQOC010000246 DAOQOC010000000
VERSION     DAOQOC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17053_length_3021_cov_3.281861"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3015
                     /locus_tag="PL106_05095"
     CDS             <1..3015
                     /locus_tag="PL106_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32476.1"
                     /translation="TGLGYDPDGSGTLYAPTPWSTTTDFNYWGHLGTDSWTAIVSHTN
                     GSRIWDYQWDEDDGANSDDCLEDNWRQRQCDFQVTTDPAGDIIVCGNAGRNMDDFYLA
                     KIQPSCDAKADYAIFEAMYPNLGADNNEVHITTAVVWNQSMNVRGSIIIDPGGSLNIT
                     GANTVIRFADSRKMGYTCNIVVNPASTINGTNSGKLVINEATLTSLDECPESMWDGIR
                     ILGWNYNDALNSVGYARIVNGGTISNAVVAATNCQVDPMDPSMAAATQVGGVIRCDDA
                     YFLRNRFDVVCRPYGFNGGPESPSNCRFINTAFETPGLLNYPNEYPETHLWLEGARRT
                     HVQSCRFGNTYNFAGVPPELWGVGIRSINTSLSVREEFEGAGWGSFTGLERGIHYEIT
                     STHTCLIRNNLFDRCAGGVRLNNTTNAVLDENEFVVPDALTTGTGFPWAYGFGIWGGS
                     GFTIENNHVRGDGPIALNPVTIGAIFDNTGNITDQTNEYANNTFDQLTYGTFIQQVND
                     GPGPDDGLHLKCNDYGTGYPNSQNFRDIAFTGPTPRVGDKQGAPYDGINQQLLAGNTF
                     TLCDESDLLTNMVEDIADVNMFTYWHHAPTPGFNLNAHCRLPGLANFQPDVLLYNKEE
                     ACGQFMMAMVGGGGGQASRMQQAQEEYALVRTVYDSQRDGGNTASLKEYVYDPAHSSS
                     EVRNTLMLAAPKVSIETWAEVFKREAPLNPWHMAQALLANSPLQPEVVRMMEESELTP
                     YYKQLVLGGQGDGINSQTILESEMSHWKHEHAEALKRLVAEALEAETTEAIDAALTVE
                     AQYPEMGTPWSRIHLLIAKGDLAGAKAIVDELNAVAEPEGAMEVLGMSLDLEMDGTPL
                     ADAPPAMLERLQYLADNEGTGMFAAQSWLHLLQPDAYPERILLPVDTRSSFAGPVDGD
                     PVATDEVISVYPNPAKDQAAVYVVVKLHEGMEKGTVQVFDPIGRLVHTEQVASSTAIV
                     EVPVQGFVPGLYLVALDGEGLRIGTGKFELTR"
BASE COUNT          672 a          926 c          902 g          521 t
ORIGIN      
        1 accgggctgg gttatgatcc cgacggttcg ggcacgctat acgcgccaac accttggtcc
       61 accaccaccg acttcaacta ctggggccac ttgggcacgg acagttggac ggccatcgtc
      121 agccacacca atggctcccg catctgggac taccaatggg atgaggatga cggggccaac
      181 tcggacgatt gcctggaaga caactggcgc cagcggcaat gcgacttcca ggtgaccacc
      241 gacccggcag gggacatcat cgtatgcggg aacgccggga ggaacatgga cgacttctac
      301 ctggccaaga tccaaccgag ttgcgatgcc aaggccgact acgccatctt cgaggccatg
      361 tatccgaacc tcggagcgga caacaatgag gtccacatca ccaccgccgt tgtctggaac
      421 cagagtatga acgtgcgggg aagcatcatt atcgatccgg gggggagctt gaacatcacc
      481 ggggcgaaca cggtgatccg cttcgccgac agccgaaaaa tggggtacac ctgcaacatc
      541 gtggtgaatc cggcctccac catcaacggg accaactcgg gcaagctggt catcaacgag
      601 gccacgctca cctccctgga cgaatgcccg gagagcatgt gggacgggat ccgcatcttg
      661 ggctggaact acaacgatgc gttgaattcg gtgggatacg ctcgcatcgt caatggtggt
      721 accatcagca atgccgtggt tgccgccacc aattgccagg tggatcccat ggatcccagc
      781 atggccgcag cgacccaggt gggcggggtg atccgctgcg acgatgcgta cttcctgagg
      841 aaccggttcg atgtggtatg taggccgtac ggtttcaacg gcgggcctga aagcccttcc
      901 aattgccgct tcatcaacac cgccttcgaa accccgggcc tcttgaacta cccgaacgag
      961 taccccgaga cccacctctg gctggaaggc gcccgacgca cccatgtgca gagctgccgc
     1021 ttcggcaaca catacaattt cgcgggtgtg ccaccggaac tgtggggcgt gggtatccga
     1081 agtatcaata cctccctcag cgtacgcgag gaattcgagg gggccggctg gggcagcttc
     1141 acgggcctcg aacgcgggat ccattacgag atcacctcca cgcacacctg cttgatccgc
     1201 aacaacctct ttgatcgctg cgccggtggc gtgcgcctga acaacaccac caacgccgtg
     1261 ctcgacgaga atgagttcgt ggtgccggac gcactcacca ccggaacggg cttcccgtgg
     1321 gcgtacggtt tcggcatctg gggcggctcg ggtttcacca tcgagaacaa ccacgtgcgg
     1381 ggcgatggcc ccattgcgct caaccccgtt accatcggtg ccatcttcga caacaccggc
     1441 aacatcacgg accaaacgaa cgagtacgca aacaacacct tcgaccaact cacctacggc
     1501 acattcatcc aacaggtgaa cgatgggcct ggcccggacg atggcctcca cttgaagtgc
     1561 aacgactacg gcaccggcta ccccaacagc cagaatttcc gcgatatcgc cttcaccggg
     1621 ccgacaccac gcgtggggga caagcaagga gcaccttacg atggcatcaa ccaacaactg
     1681 ctcgcgggca acaccttcac actctgcgat gagtcagacc tcctcaccaa catggtcgag
     1741 gacatcgccg acgtgaacat gttcacgtat tggcaccatg cgccaacgcc cggctttaac
     1801 ctgaatgctc attgccgatt gccgggcttg gccaatttcc agccggacgt actcctttac
     1861 aacaaggagg aagcctgcgg ccaattcatg atggccatgg tcggcggagg cggaggccag
     1921 gcgagccgga tgcagcaagc ccaagaggaa tacgccctgg tgcgcaccgt gtacgacagc
     1981 cagcgcgatg gcggcaacac cgcatcgctt aaggagtacg tgtacgaccc ggcgcatagc
     2041 agcagcgaag tgcgcaatac cttgatgctc gctgcaccca aggtgagcat cgagacctgg
     2101 gctgaggttt tcaaacggga agccccgctc aatccttggc acatggccca agccctgctg
     2161 gccaacagcc ccttgcagcc cgaggtggtg cgcatgatgg aggaaagtga actcacgcct
     2221 tactacaagc aactcgtatt gggcggccaa ggtgacggca tcaactcgca gaccatcctg
     2281 gagagcgaaa tgagccactg gaagcacgag catgccgagg cgctgaagcg cctggtggcc
     2341 gaagccctgg aggcggaaac cacggaggcc atcgatgcgg cgctcaccgt ggaggcccag
     2401 tatcccgaaa tgggaactcc gtggtcgcgc atccacctgc tgatcgccaa aggcgacctg
     2461 gccggggcga aagccatcgt ggacgagttg aacgctgtgg ctgagccgga gggggccatg
     2521 gaagtgctgg gcatgagcct ggatctggaa atggacggaa cccctcttgc cgatgcaccc
     2581 ccggccatgt tggaacggtt gcaatacttg gccgacaacg agggtacggg catgttcgcc
     2641 gcccaaagct ggttgcacct cttacaaccg gatgcctacc cggagcggat cctgctgccg
     2701 gtggacaccc gcagttcgtt cgcgggtcct gtggatggcg atcccgtggc caccgatgag
     2761 gtcatcagcg tgtatcccaa cccggccaag gatcaggcgg ccgtgtacgt tgtagtcaaa
     2821 ttgcacgagg gtatggagaa gggaacagtg caggtgttcg acccgatcgg ccgtcttgtt
     2881 catacggagc aagtcgccag ttcaacggct attgttgaag tgccagtgca aggattcgtt
     2941 ccgggactct acttggtggc gctcgatggt gagggtttgc ggatcgggac cggcaaattt
     3001 gaactgaccc ggtaaccatg t
//
LOCUS       DAOQOC010000247         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17084_length_3018_cov_3.668242, whole genome shotgun sequence.
ACCESSION   DAOQOC010000247 DAOQOC010000000
VERSION     DAOQOC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17084_length_3018_cov_3.668242"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PL106_05100"
     CDS             <1..589
                     /locus_tag="PL106_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA32477.1"
                     /translation="LADVNDMKMGDPRDFSNFIGAVIDERAFDRISAYIEGLKQDKKN
                     VKILAGGKCDKKTGYFIEPTVVETKDPKSITMCEEIFGPVLTIYVYGENRWMDTLKLV
                     DSTGEYALTGAVISNDRESIAEAMEVLRHSAGNFYINDKPTGAVVGQQPFGGARGSGT
                     NDKAGSYLNLIRWVSPRTIKETFVPPTDHRYPFLG"
     gene            complement(667..>3018)
                     /locus_tag="PL106_05105"
     CDS             complement(667..>3018)
                     /locus_tag="PL106_05105"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA32478.1"
                     /translation="TIKAQGEAVKRVLVDGEEFFGDDANIALKNLPAEVIDKVQVFDK
                     PSDQAQFTGFDTGDPEKTLNIVTKAGRNKGLFGRSMAGYGTDERYLASLSLNRFKGPQ
                     RITLLVQSNNINQQNFSSQDLVGLSSGGGRGGMRGASGFLTGTQPGINTTNSVGLNYS
                     DKFSKSTKLSGSWFYNNQNSVNNSASDRTTYLSDSTEQRTVQHSDRGSVNHNHRASFR
                     FEHALDSLNSFVFAPRVGFQRNTTRSNSGSSVTDDDASALSSSSTESDSQRDGIDLSS
                     DLLFRHRTRVKGRTFSVRALNSFTSQSGRSTLDAHNTFHLDTSAVVLRQRSSTTSRAQ
                     RHSLELNWTEPVGENGQLQFTMEPRIQLSNSEKLTFDVDGDQQAQAMDTLLSNKADNG
                     TRTLKSGLSYRIKLGQWKLTSGLDAQGTWMTSQQTFPHPYEVHRDYVNLLPNATISWR
                     DPRGTRVRFSYWTSTSTPSISQLQTVVDNSDPLKLSTGNLALGQGYSHMLRVQFNKLD
                     SAKTHPLFVMLNLTTQQDRISNVTYAPRTDSVLADGSLLRAGAQLSLPGNLDGYLSAR
                     GLINYGLPWLDIGSNVNVNAGGSWERLPGAVNGTRSFNTNTNLNVGAVITTNIAQGID
                     ARLGYTANHNTARSELRPSSNNSYYQGQLTARITLNGTIGWLVESEVNYNQYVGLGAG
                     FDQDVLVWNGALGWKFLKNDAMEAKITCFDILGRNASVSRTVGDTYVENAVTSMLRRY
                     LLFTLGFNLRAFKGVPEDDGPDDGPGMHRPPPGGRPPGDGPPR"
BASE COUNT          544 a          861 c          961 g          652 t
ORIGIN      
        1 gctggccgat gtgaacgaca tgaagatggg cgacccacgc gacttctcga acttcatcgg
       61 tgcggtgatc gatgagcgcg ccttcgatcg gatcagtgcg tacatcgaag ggctgaaaca
      121 ggacaagaag aacgtgaaga tcctcgcggg cggcaagtgc gacaagaaga ccgggtactt
      181 catcgagccc acggtggtgg agaccaagga tccgaagagc atcaccatgt gcgaggagat
      241 cttcgggccg gtactgacca tttacgtgta tggcgagaac cggtggatgg acacgctcaa
      301 gctggtcgat tccacgggcg agtacgcgct cactggagcg gtgatcagca acgatcgcga
      361 gagcatcgcc gaggccatgg aggtgttgcg ccacagtgcg ggcaatttct acatcaacga
      421 caagcccact ggggcggtgg tgggccagca acccttcggc ggtgcgcgcg gcagcggtac
      481 caacgacaag gccggcagct acctcaacct gatccgctgg gtgagcccgc gcaccatcaa
      541 ggagaccttc gttccgccga ccgaccatcg atacccgttc ctggggtgaa agagcgggga
      601 tgaagagatg cgagggggca ggggatggcc tcctcggacc ctcacatttt ttttccgaac
      661 acgggatcac cgcggcggcc catcgcccgg tggccttcct cctggtggcg gccggtgcat
      721 gccgggccca tcgtccggtc catcatcctc gggcacacct ttgaacgcac gcaggttgaa
      781 gcccagggtg aaaaggagat aacggcgcag catgctcgtg accgcgttct cgacgtacgt
      841 gtcgcccacc gtacgtgaaa cactcgcgtt ccggccgagg atatcgaagc aggtgatctt
      901 cgcttccatc gcgtcgttct tcaggaactt ccagcccaag gccccgttcc atacgagcac
      961 atcctgatcg aagccggcac caaggcccac gtactggttg tagttcactt cgctttccac
     1021 gagccagccg atggtaccgt tcagggtgat cctcgccgtg agttggccct ggtagtatga
     1081 attgttgctg gatggccgca gttcgctgcg cgcggtgttg tggttggcgg tgtaccccag
     1141 gcgcgcatcg atgccttgtg cgatgttggt ggtgatcacc gcgcccacgt tcagattggt
     1201 gttcgtgttg aagctgcggg tgccgttcac cgcccccggc aggcgctccc acgatccacc
     1261 ggcattcacg ttcacattac tgccgatgtc gagccaaggc agaccatagt tgatcagccc
     1321 acgtgcgctg aggtatccgt caagattccc gggcaggctc agctgtgccc cggcgcgcag
     1381 cagggaacca tcggccagta cgctgtccgt ccgcggtgca taggtcacat tgctgatgcg
     1441 gtcctgttgc gtggtcaggt tgagcatcac gaaaagcggg tgcgttttcg cgctgtccag
     1501 cttgttgaac tgcaccctca gcatgtgcga ataaccttgt cccagtgcga ggttcccggt
     1561 ggagagcttc aaggggtcgc tattgtccac aacggtctgc agctggctga tggaaggtgt
     1621 gctggtgctc gtccaatagg agaaccggac acgcgtacca cgtggatcac gccaggagat
     1681 ggtggcgttc ggcaggaggt tcacgtagtc acgatgcacc tcgtacggat gcgggaaggt
     1741 ctgctggctg gtcatccagg ttccctgcgc atcgagcccg gaggtcagct tccattggcc
     1801 aagcttgatg cggtagctga ggccgctttt gagcgttcgt gttccgttgt cggccttgtt
     1861 gctcaaaagg gtgtccatgg cctgggcctg ctggtcgcca tccacgtcga aggtgagttt
     1921 ttccgaattg ctcaactgga tccgcggttc catcgtgaat tggagctgtc cattttcgcc
     1981 aacgggttcc gtccagttga gttccaggga atggcgctgc gcccgcgatg tggtgctgga
     2041 gcgctgccgg agcaccacgg ccgaggtatc cagatggaaa gtattgtgcg catcgagggt
     2101 ggaacggccg ctttgcgagg tgaacgaatt gagtgcccgc acgctgaacg tgcgcccttt
     2161 cacccgtgtg cgatgacgga agagcaggtc gctgctcagg tcgatcccgt cgcgttggct
     2221 atcgctttcc gtggatgagg aggagagcgc cgaagcatca tcgtcggtga cggaagaacc
     2281 ggaattgctc ctcgtggtat tgcgctggaa ccccacgcgc ggcgcgaaca cgaaggagtt
     2341 caggctgtcc agggcatgct cgaaccggaa cgatgcgcga tggttgtggt tcacggatcc
     2401 ccggtcgctg tgctgcacgg tccgctgctc cgtactgtcg ctcaggtagg tggtgcgatc
     2461 gctcgcgctg ttgttcacgc tgttctggtt gttgtagaac cagctgccgc tgagtttggt
     2521 gctcttgctg aacttgtccg agtagttgag accgacgctg ttggtggtgt tgatccccgg
     2581 ttgcgtaccg gtgaggaatc cactggcgcc ccgcatgcca cctcgtccac cgccggatga
     2641 gaggccaacc aggtcctgcg agctgaagtt ctgttggttg atgttgttgc tctgcacgag
     2701 cagggtgatc cgttgtggtc ctttgaaacg gttcaggctg agggatgcga ggtagcgctc
     2761 atccgttcca tagccggcca tcgacctgcc gaaaagccct ttgttacgcc cggccttggt
     2821 cacgatattc aacgtttttt ccggatcacc ggtatcaaat ccggtgaatt gcgcctggtc
     2881 gctgggtttg tcgaagacct gtaccttgtc gatcacctcg gcgggcaggt tcttcagggc
     2941 gatgttggcg tcatcaccga agaactcctc gccgtccacc agtacgcgct tcaccgcttc
     3001 gccctgtgcc ttgatggt
//
LOCUS       DAOQOC010000248         3011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17145_length_3011_cov_3.680988, whole genome shotgun sequence.
ACCESSION   DAOQOC010000248 DAOQOC010000000
VERSION     DAOQOC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17145_length_3011_cov_3.680988"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="PL106_05110"
     CDS             complement(<1..1939)
                     /locus_tag="PL106_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA32479.1"
                     /translation="MRVRAREGAWTRPSSFSRFHPLAFALFILALFSTTRIQAQWDTL
                     LLPFRALTIEDGLSQGMVNAIIQDKYGFMWLATKDGLNRYDGYSFTVFRHDPEDSATV
                     RNNYIYALLEDEQGRLWVGTAEGLDLFDRRTETFIHVQAGREHIRNIVQSIAQDASGD
                     LWVGHNHGVVKFTFTGGKGANGEPACSSRNFLESTCFVSADRSGTIWVGQLDLAGFRI
                     TPEHMGNDRVDTLHLDRPAGRDRTGRDLYDLTGLTVVEDTINNRLFGLYMYGVVELER
                     GSTEVRTLSQVSPQLGQIRGANATVDEKGRLWIAVFTGIYRFDPEVRTWCLAMPTDQN
                     LRPMAQLAKCSYRDRNGTLWLGTAGFGAFTYEPRTGRFHTVNTASCGRMVPLPDGRVL
                     ISYFDGFLNVFDPRSRSWPVSIRWNEKEDHPLFATLNRTARGPVRDDQGIYWFNHAGI
                     MSYDPVIDRITAYPRDPIARAAFPEEDYNEMLLLEGDSNIWSGTPHTLCRFDRRTSTY
                     HHVPYPRSRLGDTEQFLHALHRADDGVLWLGTATGLMSYDRHADPAKRWKVFTNDPSD
                     AGSLSADMVYTIQADPRDANTLWIGTNGGGLNRLDKRTGRSTRYSIKDGLPNDVVYGI
                     LNDDEGQLWMSTNKGLARFSLA"
     gene            complement(1951..2598)
                     /locus_tag="PL106_05115"
     CDS             complement(1951..2598)
                     /locus_tag="PL106_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA32480.1"
                     /translation="MIRIALADDQHLFRKGMAMLLRDMADAQVVLECANGAELLDALK
                     NIPVDIVLLDLQMPVLDGIGATERIKAEFPHVKVIILSTHDEDKFIAHLMELGANGYM
                     LKTAEPDEIDTAIRAVAQSGFYLNDAVDKALLHGLVKKKQVKPVFQEIDPLSDRELEV
                     LRGICQELTTAEIAEKLFVSPRTVDFHRNNLLLKTGARNAAGLVVYAMTKGIHAP"
     gene            complement(2664..>3011)
                     /locus_tag="PL106_05120"
     CDS             complement(2664..>3011)
                     /locus_tag="PL106_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNA32481.1"
                     /translation="VNTPSIDLSYLERLYKGDRARVHAWVNIYLEELPILLERMDTCI
                     QQDDAAGLVQVLHDLRPQAHYLGAREMHDILERMGSEARSRGASACTSRALELRAVCQ
                     AVERSLRTSFRPA"
BASE COUNT          567 a          920 c          909 g          615 t
ORIGIN      
        1 tggcaagtga gaatcgcgcg agccctttgt tcgtgctcat ccatagttga ccttcatcgt
       61 cgttgaggat gccgtacacc acatcattgg gcaggccatc cttgatgctg taccgtgtgg
      121 acctgcccgt gcgcttgtcc aacctgttca ggccgcctcc attggtcccg atccaaagcg
      181 tgttcgcatc cctcgggtcg gcctggatcg tgtagaccat atcagcggag agcgatcccg
      241 cgtccgacgg atcgttggtg aacaccttcc acctcttggc aggatccgcg tggcgatcgt
      301 agctcatcag tccggtggcg gtgccgagcc acagcacacc gtcatccgca cggtgcaagg
      361 cgtggaggaa ctgttcagtg tcgcccaggc gtgaacgcgg gtacggcaca tggtgatacg
      421 tgcttgttcg cctatcgaag cggcagaggg tgtgcggggt gcccgaccag atgttcgagt
      481 cgccttcgag caggagcatc tcgttgtagt cctcctcggg gaacgccgct cgcgcgatcg
      541 ggtcgcgtgg atatgccgtg atgcggtcga tgacggggtc ataactcatg atgcccgcat
      601 ggttgaacca atagatccct tgatcgtcgc ggaccggacc acgtgccgtg cgattcaagg
      661 tcgcgaacaa gggatggtct tccttctcgt tccaccggat ggacaccggc cacgaccggg
      721 agcgtggatc gaacacgttg aggaaaccat cgaaatacga gatcagcacc cggccatcgg
      781 gcaagggcac catacgacca caactggcgg tgttcactgt atggaagcgg cccgtgcgag
      841 gctcataagt gaaggcgccg aagcctgcgg tgccgagcca aagcgtgccg ttgcgatccc
      901 tgtaagagca cttggccagt tgtgccatgg ggcgcaggtt ctggtcggtc ggcatggcca
      961 ggcaccacgt gcgcacctca ggatcgaagc ggtagatccc tgtgaacacg gcgatccata
     1021 accgtccctt ttcatccacc gtggcattgg caccgcggat ctggccgagt tgcggactca
     1081 cttggctcaa ggtccttact tcagtggagc ctcgctccaa ttcgaccaca ccatacatgt
     1141 acaacccgaa aagacggttg ttgatggtat cctccaccac ggtgagcccc gtcaggtcgt
     1201 acaggtccct tccggtacgg tcccggccag cgggccgatc aaggtgaagg gtatcgacgc
     1261 gatcgttccc catgtgctcg ggggtgatcc ggaaaccggc cagatccagt tggcccaccc
     1321 agatggtccc cgaacggtcc gcactgacga agcaggtgct ctccaggaaa ttcctgctgc
     1381 tgcatgcagg ttcgccgttg gctccctttc ccccggtgaa ggtgaacttc accactccat
     1441 ggttgtggcc cacccacaga tcaccggagg catcctgcgc gatggactgc acaatgttcc
     1501 ggatgtgttc ccggcccgcc tgcacatgga tgaaggtctc cgttctccgg tcgaagaggt
     1561 ccaacccttc ggccgttccc acccacaagc ggccttgttc gtcctccagt agcgcgtaga
     1621 tgtagttgtt gcgcacagta gcactgtcct ctggatcgtg gcggaacacg gtgaacgagt
     1681 atccatcata gcggttcagc ccgtccttgg tggccagcca catgaaacca tacttgtcct
     1741 ggatgatcgc attgaccatg ccctgggaga gcccgtcctc gatggtgagc gcgcggaaag
     1801 gaaggaggag agtgtcccac tgcgcttgaa tgcgagttgt ggagaacagg gcaagaatga
     1861 agagcgcgaa agcgagaggg tgaaagcgtg agaaagagga tggtcgtgtc catgcaccct
     1921 cgcgcgctct caccctcata ccttgcgttc tcatggcgca tggatgcctt tcgtcatcgc
     1981 gtacacaacc agccccgccg cattgcgtgc gccggtcttc agcagcagat tgttccggtg
     2041 gaagtccacc gtgcgcgggc tgacgaacag cttctccgcg atctccgccg ttgtgagttc
     2101 ctgacaaatg cccctgagca cctcgagttc gcgatcgctc aggggatcga tctcctggaa
     2161 gaccggtttc acctgcttct tcttcaccag gccgtgcaac agggccttgt ccacggcatc
     2221 gttcaggtag aatccgctct gggccacggc acgtatggcc gtatcgatct cgtccggctc
     2281 cgcggttttc agcatgtagc cattggcgcc gagttccatc aggtgcgcga tgaacttgtc
     2341 ctcgtcgtgg gtgctgagga tgatcacctt cacgtgcggg aattccgcct tgatgcgttc
     2401 ggtcgctccg atgccgtcca gcacgggcat ctggagatcg agcagtacga tgtccaccgg
     2461 gatattcttc agcgcatcga gcaactccgc cccgttggcg cattcgagta ccacttgcgc
     2521 atcggccatg tcgcgcagga gcatggccat gcccttgcgg aacaggtgct ggtcatcggc
     2581 gagggcgatc cggatcaccg cggaaagatc gggagagccg gaccgatccg cacccggtat
     2641 ttccaccggt tcttggtgcc gggtcaagca ggcctgaacg acgttcgcag cgatcgctca
     2701 accgcctggc acacggcccg aagctccagt gcgcgcgatg tacaggccga agccccgcgc
     2761 gatcgcgctt ccgaccccat ccgctccagg atgtcatgca tttcgcgcgc accgaggtaa
     2821 tgcgcctgcg gtcggaggtc gtgaaggacc tggacaagac ctgccgcatc gtcctgttgg
     2881 atgcacgtgt ccatgcgttc cagcaggatc ggaagttcct ccagatagat gttcacccac
     2941 gcatgtacgc gcgcgcgatc gcccttgtac aggcgttcga ggtacgacag gtcaatggag
     3001 ggggtgttca c
//
LOCUS       DAOQOC010000249         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17234_length_3003_cov_4.287992, whole genome shotgun sequence.
ACCESSION   DAOQOC010000249 DAOQOC010000000
VERSION     DAOQOC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17234_length_3003_cov_4.287992"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PL106_05125"
     CDS             <1..808
                     /locus_tag="PL106_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA32482.1"
                     /translation="AGKPLPPGFTAIADRPGDRWSEIGLDLPEIVDGLRGHGIALGTA
                     IGYGVLVTGVLVLARIISSYAALITTFIFRPQVAPRGRTRKQTWMMPWLLGWTGMRGV
                     VSLAAALAIPVLLEDGTAFPHRSLILFITFVVILLTLVVQGLTLPYLIRTTGLFEGAP
                     ASLSGEEELHRVRHSLHAHTLAFLRERVDAEHPDHAGLRRIAAQWPAHGEADASDAGR
                     EQTKLVMLELLESQRQHLRELNRDPAIDDDLLRHEVYLIDLEEERLRMKG"
     gene            855..2441
                     /locus_tag="PL106_05130"
     CDS             855..2441
                     /locus_tag="PL106_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32483.1"
                     /translation="MHRSCAFLFLLLFWVPSQGQAPFIQALTQDTSMNLSRPRVLARI
                     APDLYRVVGGSGNGDSAWYYSADIDAAGLLANMRTMRCGVNTTNAAPSCMLRTSDQGM
                     LVAFAQPDPGTNSYSFAKLDVNNNVQWYRNYFDVYGQWMVDSQHALAEKDGHYFTFGR
                     MTTVPTTEGHASIMVELDGTGVCVVQRIWAGGDMWSGEGAGIVRTADNGLLTVATEHI
                     YSGVSSFPTMSVQRWDAALQVAWSNRYSFGYYHSVVRLMATADGGALLMGKARATLGS
                     QAYPFFLRLDGAGQVLWARLVLNSDLVPTGMVEEPDGGFAICLYDDPPAPIVARLDNT
                     GALVSAQRASSLSEPVHPWLISLDSITGEHLVGATTAAYEPTTYFMRLDAALSFACGS
                     TPYLWADSIVSPTVTAFTVTVTPAWLSSADTAWASHDTPVFGATDACLSTAVREEEPE
                     ALRVWPIPAQDVLHVAWYGSGSVPITYSLIDPTGRTMMQGPVGRDGEGLWIDVHDLGF
                     GTYLLRLETAQGRRTVRVIK"
     gene            complement(2471..2707)
                     /locus_tag="PL106_05135"
     CDS             complement(2471..2707)
                     /locus_tag="PL106_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32484.1"
                     /translation="MGEVGNAFKDFTAIRPVVNLSSRLQTAAQAGEILITSQVYDKVK
                     DEWPDLPGRSLSLKGIGGSVESNVIGVDVYRAPL"
     gene            2723..>3003
                     /locus_tag="PL106_05140"
     CDS             2723..>3003
                     /locus_tag="PL106_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32485.1"
                     /translation="MDADADGLLGTAGIIEFGDPAWVEDCCDLSAHTGLRRLAAQWEG
                     KAATGDHVPGHAETRQVMLELLESQRQHLRELNRDPAIDDDLLRHEVYL"
BASE COUNT          507 a          985 c          952 g          559 t
ORIGIN      
        1 ggcgggcaag ccgttgccgc cgggattcac cgcgatcgcc gatcgcccgg gcgatcgctg
       61 gagcgagatc gggctggatc tgccggagat cgtggacggc ctccgcggcc atggcattgc
      121 cttgggcacc gccatcggct acggcgtgct ggtgacgggg gtgctggtgc tcgcgcgcat
      181 catcagttcg tacgccgcgc tcatcaccac cttcatcttc cggccgcagg tggcaccgcg
      241 cgggcgcacc cgcaagcaga cctggatgat gccctggttg ctgggctgga caggcatgcg
      301 cggcgtggtg tcgctggcgg ctgcgctggc cataccggtg ctgctggagg atggcacggc
      361 cttcccgcac cgcagcctca tcctcttcat caccttcgtg gtgatcctgc tcacgctggt
      421 ggtgcagggc ctcacgctgc cgtacctgat ccgcaccacc ggtctcttcg agggcgctcc
      481 ggcctccttg tccggggagg aggaactcca ccgcgttcgg cacagcctgc atgcgcacac
      541 cttggcgttc ctacgcgagc gcgtggatgc cgaacatccg gaccacgcgg gcctgcggcg
      601 gatagctgcc caatggcccg cacacggcga ggctgacgcg agtgacgcgg gccgggagca
      661 gaccaaactg gtgatgctgg agttgctgga gagccaacgc cagcacctgc gggagttgaa
      721 ccgcgacccc gcgatcgatg atgacctcct ccgccatgag gtgtacctga tcgacttgga
      781 agaagaacgg ctgcggatga aggggtagat ccccttacac aaccattcca taggttgtcc
      841 gtcctttaac caacatgcac cgttcctgtg ccttcctctt cctgctgctg ttctgggtcc
      901 ccagtcaggg gcaggcacct ttcatccaag cgcttactca ggacaccagt atgaacctta
      961 gtcggccgcg tgtgttggca cggatcgccc cggacctcta ccgcgtcgtt gggggctccg
     1021 gcaatggcga ttcagcctgg tactacagcg ccgacatcga tgccgccggc ctgctcgcaa
     1081 acatgcgcac catgcgctgt ggggtgaaca ccaccaacgc ggcaccaagc tgcatgttgc
     1141 gcacttccga ccaggggatg ctcgtggcct tcgcgcagcc cgaccccggc accaactctt
     1201 attccttcgc caagctggat gtgaacaaca atgtgcagtg gtaccggaac tacttcgatg
     1261 tttacggcca gtggatggtc gattcgcaac atgccctcgc ggagaaggac ggacactact
     1321 tcaccttcgg ccgcatgacg accgttccca cgacggaggg tcatgccagc atcatggtgg
     1381 agttggatgg taccggtgta tgcgtggtgc agcgcatctg ggcgggtggc gacatgtggt
     1441 ccggcgaggg ggcgggcatc gtgcgcaccg cggacaatgg tctgctgacc gtggcgaccg
     1501 agcacatcta ttctggcgtt tccagcttcc cgaccatgag cgtacagcgc tgggacgccg
     1561 cgttacaggt ggcctggtcc aaccgttatt ccttcggcta ctatcatagc gtggtccgct
     1621 tgatggctac ggctgatggc ggtgcactgc tcatgggaaa ggccagggcc accctgggca
     1681 gccaggccta tcccttcttc cttcggctcg atggcgccgg ccaggtcttg tgggcaaggc
     1741 tggtgttgaa ctccgacctg gtgcccaccg gcatggtcga agagcctgac ggcggcttcg
     1801 ccatctgctt gtacgatgac cccccggcac cgatcgtggc gcgcttggac aataccggcg
     1861 cactggtctc cgcgcagcgc gccagcagcc tctccgaacc ggtgcatccc tggttgatct
     1921 ccctcgacag catcacaggc gaacacttgg taggcgccac cacggccgct tacgaaccca
     1981 cgacctactt catgcgcctc gacgccgcgc tttccttcgc ctgcggaagc acgccctacc
     2041 tctgggccga cagcatcgta tcaccaacgg tcacggcctt taccgtcacc gtcaccccag
     2101 cctggctgag ctctgccgat acggcctggg cctcgcatga caccccggtg ttcggcgcca
     2161 cggatgcctg cctcagcacc gcggtgcgcg aggaagagcc tgaagcactg agggtctggc
     2221 cgataccggc ccaagatgtg ctccacgtgg cctggtacgg gagcggatcg gtcccgatca
     2281 cctattcgct gatcgacccc acgggccgca cgatgatgca aggtcccgta gggcgcgacg
     2341 gcgagggctt atggatcgac gtccatgatc tgggcttcgg cacctacctc ttgcggttgg
     2401 agacggctca aggtcggcgg accgtcaggg tgatcaaata ggatcggcgt tcaacgggtc
     2461 ggcccgccgg tcacaacggc gcgcgataca cgtccacacc gatcacattg ctctccaccg
     2521 aacctccgat ccctttcaac gagagcgacc ggccgggcag atcgggccat tcgtccttca
     2581 ccttgtcgta gacctgggat gtgatgagga tctcgccggc ctgtgcggcg gtctgcaggc
     2641 gcgacgacag attgaccacc ggccggatcg cggtgaagtc cttgaaggca ttgcccactt
     2701 cgcccatgcg gctgatgccg gtatggatgc cgacgccgat ggactgctcg gtaccgcggg
     2761 catcatcgag ttcggtgatc ccgcgtgggt ggaggattgt tgcgacttgt ccgcacacac
     2821 gggcctgcgg cggctggcgg cacaatggga aggcaaggcg gccaccggcg accatgttcc
     2881 gggccatgcg gagacccggc aggtgatgct ggagttgctc gagagccaac gccagcacct
     2941 gcgggagctg aaccgcgacc ccgcgatcga tgatgacctc ctccgccatg aggtgtacct
     3001 gat
//
LOCUS       DAOQOC010000250         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17236_length_3003_cov_3.375509, whole genome shotgun sequence.
ACCESSION   DAOQOC010000250 DAOQOC010000000
VERSION     DAOQOC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17236_length_3003_cov_3.375509"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PL106_05145"
     CDS             complement(<1..559)
                     /locus_tag="PL106_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNA32486.1"
                     /translation="MKTNVTLPDGSVRSFEGAVTAMDVAKSISEGLARNVLSAKVNGE
                     VRDANRTLPETCTLALLTWNDLEGKATMWHSSAHVMAEAVEALYPGAKFGIGPAIENG
                     FYYDIDFGGQSVGEHDFAAIEAKFKELASKKETFTRREVGKADAIKYFTEKGDEYKLE
                     LIEGLNDGEITFYQQGNFVDLCKGPH"
     gene            complement(689..>3003)
                     /locus_tag="PL106_05150"
     CDS             complement(689..>3003)
                     /locus_tag="PL106_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32487.1"
                     /translation="AQATGSTSDYFLAPTYMPAWIEQPLPGGGNGCVGGYFCHDYMEY
                     IGGCLMQPMLAGESYSLNMSVAAFEIDNFLSNTTLMNLSPVNVTIFGSATCPTWPTPV
                     PLCPGTEPGWSELGYVTYTPNESWSQVTITFVPTFDVQAIMIGSPCTLPPDYPSVSDQ
                     WLAYFIFDNLTLNETSAFGSTITEAGEFYLDDLELTGHPDSLAQSYQWFYQGVALPGQ
                     TDTILGVSINDLDTGWYQMVSYYDTICVISEIHIDPICVPAVIGNAPVTSCDPVNFQL
                     QTLSDLSQVIGVNWDFGDGSSDTTSAPSHQYTEPGTYDVTLSLLSEDFCPTDTTYTAL
                     ITIYEVPEPTFTADPLEGCIGMTVNFTNTTGTLTGNCAWSFGDGGSANICDPSHTYNQ
                     ADTFSVQLTVTSPQGCTNDTTITEMIIVYAEPQVSFTSDTIAGCTPLTIQFTNTTPAN
                     QVGSVLWDLGNGTTTTTDNPSGLYTDPGTYSVSLEVTHPQGCSAEVTVQDMITAYGHP
                     VVTFTNQPDSGCYPLEVQFANTTDAAFTNTCAWNFGDGGTSDQCTTAHTYTAPGVYTV
                     SLEVISPQNCDGDTTYADLITVFDHPQADFYFGPQPTDIFNTYITFIDSSSVDALQWN
                     WSFGENGALGSSTQEHPALHFPDIRGLYPVQLVVTNVHTCTDTMLRFVEIEGYYAVYA
                     PNAFTPDGDGVNDTWRPLVRDQEDAYYHVTVYDRWGHEVWATNDPAQGWDGKANGGSI
                     VTGVYSWKLKTRDVVGNFDHEYLGHVSLLK"
BASE COUNT          584 a          818 c          951 g          650 t
ORIGIN      
        1 tgtgggggcc tttgcagagg tccacgaaat tgccttgttg gtaaaacgtg atctcgccgt
       61 cgttcaggcc ctcgatcagt tccagtttgt attcgtcgcc cttttccgtg aagtacttga
      121 tggcgtcggc cttgcccacc tcacggcggg tgaaggtctc tttcttgctg gcgagttcct
      181 tgaatttggc ctcaatagcg gcgaagtcat gctcgcccac actttggccc ccgaagtcga
      241 tgtcgtagta gaagccgttc tcaatggctg gaccgatacc gaatttggcg cctggataga
      301 gggcttccac cgcttcggcc atcacgtggg cgctgctgtg ccacatcgtg gcttttccct
      361 cgaggtcgtt ccaggtgagc aaggccaggg tgcaggtttc gggcagggtg cggttggcgt
      421 cgcgcacttc accgttcacc ttggcggaaa gcacattgcg ggccaggccc tcactgatgc
      481 ttttggccac gtccatggcc gttaccgcac cttcaaaact gcggacggaa ccgtccggca
      541 gggtcacgtt ggtcttcatc gatcatcctt gaccgcccta cgagcggcgg caaaagggcg
      601 gcgaaggtaa gtattggaag gacagtgtgt tgagtgctcc gaccaggttg ctccgcacat
      661 cagggtcagg ggacccgagg tattgatgtc acttcagcag actcacatgc ccgaggtact
      721 catgatcgaa attcccgacc acatcccgcg tcttcagctt ccagctgtac acgccggtca
      781 cgatcgatcc gccgttcgcc tttccatccc acccttgtgc gggatcattg gtggcccata
      841 cctcatggcc ccagcgatcg taaacggtaa cgtggtaata agcgtcctcc tgatcccgca
      901 ccagcggccg ccatgtgtcg ttcacgccgt cgccgtccgg ggtgaatgcg ttgggtgcgt
      961 acacggcgta ataaccctcg atctccacga agcgcaacat cgtgtccgtg caggtatgca
     1021 cgttggtcac caccagttgt accgggtaga gtccacggat atccgggaag tgcaacgcgg
     1081 gatgttcctg ggtgctggag cccagcgcgc cgttctcgcc aaaactccag ttccactgca
     1141 gggcgtccac actgctggag tcgatgaagg tgatgtaggt gttgaagatg tccgtgggct
     1201 gcggaccgaa gtagaagtcg gcctgcggat gatcgaagac cgtgatcagg tccgcatagg
     1261 tggtatcgcc gtcgcagttc tggggcgaga tcacctcgag tgagacggtg tacaccccgg
     1321 gtgccgtgta ggtatgagcg gtcgtgcatt ggtcggaagt gccaccatca ccgaagttcc
     1381 atgcgcaggt attggtgaac gccgcatccg tggtgttggc gaactgcacc tcgagcgggt
     1441 agcagccgct gtccggctgg ttggtgaagg tgaccaccgg gtgaccataa gcggtgatca
     1501 tgtcctgtac ggtcacctcg gccgagcaac cctgtggatg ggtcacttcc aaactcacgg
     1561 aatacgtacc aggatcggtg tatagcccgg aagggttgtc ggtggtcgtt gtggtgccgt
     1621 tgcccaggtc ccagagcacg ctgcccacct ggttggccgg agtggtgttc gtgaactgga
     1681 tggtcaacgg ggtgcagccg gcgatggtat cactggtgaa gctgacctga ggctcggcgt
     1741 aaacgatgat catttcggtg atcgtggtat cgttcgtgca gccctgtgga ctcgtaacgg
     1801 tcaactgcac gctgaaggtg tcggcctggt tataggtgtg cgaaggatcg cagatgttgg
     1861 ccgaaccgcc atcaccgaag ctccacgcgc agttgcccgt gagcgttccg gtggtgttgg
     1921 tgaagttcac cgtcatgccg atgcaacctt ccagcggatc ggccgtgaag gtgggttcgg
     1981 gcacctcgta gatggtgatg agcgcggtat aggtagtgtc cgttggacaa aaatcctccg
     2041 acaggaggga caaggtcacg tcataggttc ccggttcggt gtactgatga ctcggggcgc
     2101 tcgtggtgtc cgaggatccg tcgccgaagt cccaattgac acctatcact tgggagagat
     2161 ccgacaaggt ttgaagttgg aagttcaccg ggtcgcaact ggtcaccggc gcgttgccga
     2221 tcacggcggg tacacagatc gggtcgatgt ggatctcgct gatcacgcaa atggtgtcgt
     2281 agtaactcac catctggtac catccggtgt ccagatcgtt gatggagacg cccaggatgg
     2341 tgtccgtttg gccgggcagg gcaacgccct ggtagaacca ctggtagctc tgggccaagc
     2401 tgtccgggtg cccggtgagt tccaggtcgt ccagatagaa ttcaccggcc tccgtgatgg
     2461 tggagccaaa ggccgaagtt tcgttcaacg tgagattgtc gaagatgaag taggcgagcc
     2521 actgatcgga cacgctggga tagtcggggg gaagtgtgca aggcgaaccg atcatgatgg
     2581 cctgcacgtc aaacgtgggt acgaacgtga tcgtgacctg gctccaggac tcgttcggtg
     2641 tgtaggtcac atagcccaat tcggaccagc caggctcagt tccaggacaa agagggaccg
     2701 gagtaggcca ggtcggacat gtggcggacc cgaagatcgt cacgttcacc ggactcaagt
     2761 tcattaaagt ggtgttgctc aggaaattgt cgatctcgaa agcggccacc gacatgttca
     2821 gactgtacga ttcgcctgcc agcatgggct gcatcaggca gccgccgatg tactccatgt
     2881 aatcgtggca gaagtaaccg cccacgcaac cattgccacc acctggcaag ggttgctcga
     2941 tccaagctgg catgtaggtg ggcgccagga aatagtcgga ggtggaaccg gtcgcctggg
     3001 ccc
//
LOCUS       DAOQOC010000251         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17320_length_2995_cov_4.316667, whole genome shotgun sequence.
ACCESSION   DAOQOC010000251 DAOQOC010000000
VERSION     DAOQOC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17320_length_2995_cov_4.316667"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PL106_05155"
     CDS             complement(<1..1218)
                     /locus_tag="PL106_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32488.1"
                     /translation="MERHINLLLAVLGIGYGIFAQSPSRIWDHIVSMGYDIPTNHNVM
                     LGFNGDALPSVTVLENTASYQSPLAVSDPVTGALKFIMARNALFDATGNETPNSGLWA
                     SAANGHGYVTVIPHPGARDRYYLITRVCGIPCSEYRWTLYDGTLNGGLGDVDPELVDQ
                     PWFTGGIGDLHGMIASADGNTYWFITHEPGNADWTLHPITAAEGIDADPVTITGGPLI
                     TDASSYTSMWMVVAPSNNKLALRYKSGGTFQLALLGFDNVLGTFNGMEATLPYSTERR
                     ITFSPGGTYMYKEGVNDLGEGMVDQYDLEAGDGTAIWNSRQQAAIPDLQGQQMASLLF
                     SNTADGRIWIEGITNTMYFTHKIIPINEPDLPAPACDVSQGLELGVEFISSFWLPTMW
                     WPVLSDASALDETG"
     gene            complement(1232..1747)
                     /locus_tag="PL106_05160"
     CDS             complement(1232..1747)
                     /locus_tag="PL106_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32489.1"
                     /translation="MIMLALGPLALRAQVTYTVDNYTSDPFQKVYVYYNVIGGGASTY
                     VVTGIPIGTNTINLPSNCDYITGVRIYCYSWSVLSYVHNNSYYCGAQDPYTPPCYAGR
                     YTNNYKCPPDGVSGTTGCDSGSYCIYYQHCNDDNWDGSTGHCMNNGALRIIDLGQNFC
                     PGGGSPRCEFP"
     gene            complement(1962..2162)
                     /locus_tag="PL106_05165"
     CDS             complement(1962..2162)
                     /locus_tag="PL106_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32490.1"
                     /translation="MAKKRPSKRKGGKRAPKKDLAAIDRAAKREAQKDQGAFDGRYRP
                     RVVKSKKQYSRKRTRKDDTGAE"
     gene            complement(2191..2721)
                     /locus_tag="PL106_05170"
     CDS             complement(2191..2721)
                     /locus_tag="PL106_05170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNA32491.1"
                     /translation="MKKADHLIIRPVSIADADALYALIERDRARYTAYFQVTTAGTVD
                     PAMTLAYVHFLMAQAASGETFCHLIRPEGSHDPVGIVFLRSFDRHTGKCEVAYLVSSP
                     HTNKGIATAAVAWAVDEAFRTHGMNKVFARVDPDNIASIRVVEKNGFQREGLLRQDFR
                     THDGHLLDVIVFGKLR"
     gene            complement(2782..>2995)
                     /locus_tag="PL106_05175"
     CDS             complement(2782..>2995)
                     /locus_tag="PL106_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32492.1"
                     /translation="VVTYVAFWVFKGWSFALCTALVITAYSYKLIVALLSTPLVYLAH
                     GAAERYLGKERAHAMREAALGQADEL"
BASE COUNT          558 a          876 c          920 g          641 t
ORIGIN      
        1 gcccgtttcg tccaacgcgc tcgcatcgct cagcaccggc caccacatgg tcggtaacca
       61 gaacgaggaa atgaactcca cgcccaattc caacccctgc gacacatcgc aggccggtgc
      121 cggaaggtcc ggttcgttga tcggaatgat cttgtgcgtg aagtacatcg tgttggtgat
      181 accctcgatc caaatgcgcc catcagccgt attggagaac aggaggctgg ccatttgctg
      241 gccctgcaga tccggtatgg cggcctgttg cctgctgttc cagatggccg ttccatcgcc
      301 cgcttccagg tcgtactggt ccaccatgcc ctcacccagg tcgttgacgc cttccttgta
      361 catataggtg cctcccgggg agaacgtgat ccgcctctcg gtactatatg gcaaggtcgc
      421 ctccatgcca ttgaacgtgc cgagcacatt gtcaaagccg agaagtgcaa gttggaatgt
      481 gcctccggac ttgtagcgga gagcgagttt gttgttggaa ggcgccacga ccatccacat
      541 gctcgtatag ctggatgcgt ctgtgatcaa cgggccgccg gtgatggtca ccggatcggc
      601 gtcgatgccc tcggctgcgg tgatcgggtg cagcgtccaa tcggcattgc ccggctcatg
      661 ggtgatgaac caataggtgt tcccatcggc actggcgatc atgccgtgca gatcgccgat
      721 gccgccggtg aaccagggtt ggtccaccag ctcggggtcc acatcgccca gcccaccgtt
      781 gagtgtgccg tcatacagtg tccaacggta ctcactgcat ggtatgccgc acacccgtgt
      841 gatcaggtag tacctgtcgc gagctccggg gtgtgggata accgtgacgt atccgtgtcc
      901 attagccgct gatgcccaaa gaccactgtt cggggtttcg ttcccggtgg catcaaacag
      961 ggcattgcgc gccatgatga acttcaaggc gcctgtaacg gggtccgaaa ccgcaagggg
     1021 tgactggtag gaagccgtgt tctccagtac agtcacgctc ggcaaggcgt ccccgttgaa
     1081 gccgagcatc acgttgtggt tcgtgggaat gtcatagccc atgctcacga tgtggtccca
     1141 gatccgagag gggctttgcg cgaaaatgcc gtaaccaatg cccagcacgg caagtagtag
     1201 gttgatgtgg cgttccatgg tcgtgcgggt gttaggggaa ttcgcaacgg ggtgatccgc
     1261 cgcccgggca aaagttctgg ccgagatcga tgatgcgtaa ggctccgttg ttcatgcaat
     1321 ggccagtgct gccgtcccaa ttgtcatcgt tgcagtgctg gtagtagatg cagtagctgc
     1381 cgctgtcgca gcccgtggtg ccactcactc catccggcgg acacttgtag ttgttcgtgt
     1441 atcggcctgc atagcaggga ggtgtgtacg ggtcctgcgc tccgcagtag tagctgttgt
     1501 tgtgtacata ggacagcaca ctccaactgt agcaatagat ccgtacgccg gtgatgtagt
     1561 cacagttgct cggcaggttg atggtgttgg tgccgatcgg gatcccggtg acaacgtagg
     1621 tagaggcacc gccgccgatc acgttgtagt acacgtacac cttctggaag ggatcactgg
     1681 tgtaattgtc caccgtgtag gtcacttgcg cgcgaagggc gagcggccct aacgcaagca
     1741 tgatcatgat gggcgcgagg cgccatgcct tgtggtcggg taccgttcta cggcccggcc
     1801 atggcccgcg agtttcgcgg gggggggtga atgtttcatt ctttttcgga atggtacatc
     1861 gaaggtatac gaaaattccg gacaggaaca catatgctcg tgcgctcaac ccggaggaac
     1921 gatccggctc ctgacaggcc ggacccagcg ggacgttgtg atcactcggc tccggtatcg
     1981 tctttccggg tacgcttgcg cgagtactgt ttcttgctct tcaccacccg tggtcggtag
     2041 cgcccgtcga aagcgccctg gtccttctgg gcctcgcgtt tcgccgcccg gtcaatggcc
     2101 gccaggtcct tcttcggcgc gcgttttcca cctttgcgtt tggagggcct cttcttcgcc
     2161 atggccctgc gaagatggtg atcgggttga tcaacgcagc ttgccgaaga cgatcacatc
     2221 cagcaggtgc ccgtcatgcg tacggaagtc ctggcgcaaa agcccttctc gctggaatcc
     2281 gttcttctcc accacgcgga tgctcgcgat gttatcggga tccacgcgtg cgaacacctt
     2341 gttcatcccg tgcgtgcgga aggcttcgtc aacggcccat gccactgcgg ctgttgcgat
     2401 acccttgttc gtatggggcg aggaaaccag ataggccact tcgcatttgc cggtgtgccg
     2461 atcgaaggag cgcaggaaga cgatccccac gggatcgtgc gaaccttccg gccggatcag
     2521 atgacagaag gtctcgcccg aggcggcctg cgccatcagg aagtgtacgt atgccagggt
     2581 catcgcggga tcgaccgtgc cggcagtggt cacttggaag taggcggtgt accgcgcccg
     2641 gtcacgttcg atgagtgcgt agagcgcgtc cgcatccgcg atggatacgg gtcggatgat
     2701 caggtggtcg gcttttttca tcgccgggag cgggctgcgg cgaagatggg gccaactacc
     2761 cggaaatggc acggaccgtc cctatagctc gtccgcctgc cccagtgccg cttcccgcat
     2821 ggcatgtgcc cgctccttgc cgaggtagcg ctccgcagca ccatgcgcca gatacaccag
     2881 cggtgtggac agcaaggcca cgatcagttt gtagctgtac gcggtgatca ccaacgccgt
     2941 acacagcgcg aagctccagc ccttgaacac ccagaaggcc acataggtca ccacc
//
LOCUS       DAOQOC010000252         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17443_length_2984_cov_3.517241, whole genome shotgun sequence.
ACCESSION   DAOQOC010000252 DAOQOC010000000
VERSION     DAOQOC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17443_length_2984_cov_3.517241"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..681)
                     /gene="rplC"
                     /locus_tag="PL106_05180"
     CDS             complement(64..681)
                     /gene="rplC"
                     /locus_tag="PL106_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNA32493.1"
                     /translation="MSGLIGKKIGMTSIYDAAGSLVGCTVLEAGPNVVTHVKTAEKDG
                     YNAVQLAFGERGEKNSTAASKGHYKKANTTPKVKAHEFKEFETDLKLGDSVDAGIFEE
                     GGYVTVTGATKGKGFQGVVKRHGFSGVGESTHGQHDRTRAPGSLGGSSYPSRVFKGMR
                     MAGRTGGNKVTTENLQVVKIDKEKNLMVVRGSVPGPKGSYVIIWK"
     gene            complement(739..1047)
                     /gene="rpsJ"
                     /locus_tag="PL106_05185"
     CDS             complement(739..1047)
                     /gene="rpsJ"
                     /locus_tag="PL106_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNA32494.1"
                     /translation="MTTQKIRIKLRSYDHNLVDKSAEKIVKTVKSTGAVVSGPIPLPT
                     HKRIFTVLRSPHVNKKAREQFQLCSYKRLMDIYSSNSKTVDALMKLELPSGVDVEIKV
                     "
     gene            complement(1055..>2984)
                     /gene="fusA"
                     /locus_tag="PL106_05190"
     CDS             complement(1055..>2984)
                     /gene="fusA"
                     /locus_tag="PL106_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNA32495.1"
                     /translation="TSAATTTSWKYRGDTYKINLIDTPGHVDFTVEVERSLRVLDGAV
                     ALFCAVGGVEPQSETVWRQANKYKVPRIGFVNKMDRSGADFFNVVKQIRERLGANPIP
                     LQVPIGAEANFKGVVDLVNNRGMVWNEHDQGMTWNEVPIPDDLKETVEEWRAKLIEAV
                     AESDDSLMEKFFNDPNSLTEAEIMNAIRKSTISMSITPVLCGSSFKNKGVQTMLDAVM
                     AYLPSPVDIEAVTGTDPDSGEEILRKPSVSEPMASLAFKIATDPYVGRLAFFRCYSGA
                     IPAGSYVKNMRTGNKERISRIFQMHSNKQNPVDVIEAGDIGAAVGFKDIRTGDTLCAE
                     DKPIVLESMSFPEPVIGIAVEPKTQADLDKMGQALYKLAEEDPTFKVHTDDETGQTVI
                     SGMGELHLEIIVDRMKREFKVECNQGAPQVKYKEAITGSINHREVYKKQTGGRGKFAD
                     IHVTIEPQTDLTKEGLEFIDLIKGGSIPKEFIPPVEKGFKASLQNGVLAGFPMERLKV
                     TLTDGSFHNVDSDALSFEICAKSAFREAVPKCKPVLLEPIMKIEVITPEENMGDIVGD
                     LNRRRGQIQGMEDRSGAKAIKGIVPLSEMFGYVTSLRTLSSGRASSTMEFSHYENAPN
                     NITEAVLAKVKGKATAKA"
BASE COUNT          433 a          934 c          922 g          695 t
ORIGIN      
        1 gtccttgcta tggatatcga gttccattct tctcttcagt tgcgggtgac gcgggcgcct
       61 ccgttacttc cagatgatca cgtagctgcc tttcggcccg gggaccgatc cgcgcaccac
      121 catgaggttc ttctccttgt cgatcttcac cacctggagg ttctcggtgg tcaccttgtt
      181 gccgccggtg cggccggcca tgcgcatgcc cttgaacacg cggctggggt acgaactgcc
      241 tcccagcgaa ccgggggccc gggtacggtc gtgctgaccg tgggtgctct cgcccacacc
      301 gctgaagccg tggcgcttca ccacgccctg gaagcccttg cccttggtgg caccggtcac
      361 ggtaacgtaa ccgccctcct cgaagatgcc cgcgtccacg gaatcgccca gcttcaggtc
      421 ggtctcgaac tccttgaact cgtgcgcctt taccttcgga gtagtattgg ctttcttgta
      481 gtggcccttg ctggcggcgg tagaattctt ctcgccacgc tcaccgaagg cgagctgcac
      541 ggcgttatag ccgtccttct ccgcggtctt cacgtgggtg accacgttgg ggccggcctc
      601 cagtacggtg cagcccacga ggctgccagc cgcatcatag atgctggtca tcccgatctt
      661 cttgccgatg agtccgctca tggtttgttc gtttttggtg gggcggggcg aaccccgtcc
      721 ttaccgcata tctatccgtc agactttgat ctccacgtcc acgccgctgg ggagttccag
      781 tttcatcagc gcatcaacgg tcttgctgtt cgagctgtag atgtccatca ggcgcttgta
      841 gctgcacagt tggaactgct cgcgggcctt cttgttcacg tgcgggctgc gcagcacggt
      901 gaagatgcgc ttgtgggtgg gcagggggat gggaccgctc accacggctc ccgtgctctt
      961 caccgttttc acgatcttct cggcgctctt gtcgacgagg ttgtggtcgt aggaccgcaa
     1021 cttgatccgg atcttctggg tggtcatcgg ttcctcaggc tttggccgtt gcttttcctt
     1081 tcaccttggc gagcaccgct tcggtgatgt tgttgggggc gttctcgtag tggctgaact
     1141 ccatggtgct gctggcgcgg ccggagctca gggtacgcag gctggtcacg tagccgaaca
     1201 tctcgctcag gggcacgatg cccttgatgg ccttggcccc gctgcggtcc tccatgccct
     1261 ggatctgtcc acggcggcgg ttgaggtcgc ccacgatgtc gcccatgttc tcctcgggcg
     1321 tgatcacctc gatcttcatg atgggctcca gcagcacggg cttgcacttg ggcacggcct
     1381 cgcggaaggc gctcttggcg cagatctcga agctgagcgc gtcggagtcc acattgtgga
     1441 agctgccgtc ggtgagggtc accttcaggc gctccatggg gaagccggcg agcaccccgt
     1501 tctggaggct ggctttgaaa cccttctcca cgggagggat gaattccttc gggatcgatc
     1561 cgcccttgat caggtcgatg aactccagcc cctccttggt caggtcggtc tgcggctcga
     1621 tcgtgacatg gatgtcagcg aacttgcctc ggccaccggt ctgcttcttg tacacctcgc
     1681 ggtggttgat gctgccggtg atggcctcct tgtacttcac ctggggcgca ccctggttgc
     1741 actccacctt gaactcgcgc ttcatgcggt ccacgatgat ctcgaggtgg agttcgccca
     1801 tcccgctgat cacggtctgg ccggtctcgt cgtcggtgtg taccttgaag gtgggatcct
     1861 cctcggcgag tttgtagagg gcctggccca tcttgtccag gtcggcctgg gtcttgggct
     1921 ccacggcgat gccgatcacg ggctcgggga agctcatgct ctccagcacg atgggcttgt
     1981 cctcggcgca gagcgtatcg ccggtgcgga tatccttgaa acctaccgcc gcaccgatat
     2041 cacccgcctc gatcacatcc accgggttct gcttgttgga gtgcatctgg aagatgcggc
     2101 tgatgcgctc cttgttgccg gtacgcatgt tcttcacgta gctgccggcc gggatggcac
     2161 cgctgtaaca acggaagaag gcgagacggc ccacgtaggg gtccgtggcg atcttgaagg
     2221 ccagggaggc catgggctcg ctcacactgg gcttgcgcag gatctcctcg ccgctgtccg
     2281 gatcggtgcc agtgaccgct tcgatgtcca ccgggctggg caggtaggcc atcacggcgt
     2341 ccagcatggt ctgcacgccc ttgttcttga acgagctgcc gcacagcacg ggcgtgatgc
     2401 tcatgctgat ggtgctcttg cggatggcgt tcatgatctc ggcctcggtg agggagttcg
     2461 gatcattgaa gaacttctcc atcaggctgt cgtcgctctc ggcaacggcc tcgatgagct
     2521 tggcgcgcca ctcctccacg gtctccttca gatcgtcggg gatgggaacc tcgttccagg
     2581 tcatgccctg gtcatgctcg ttccacacca tgccgcggtt gttcaccagg tccaccacgc
     2641 ctttgaaatt ggcctcggcg ccgatgggca cctgcagggg gatggggttg gcacccaagc
     2701 gctcgcggat ctgcttcacc acattgaaga agtccgcacc gctgcggtcc atcttgttca
     2761 cgaagccgat gcggggcacc ttgtatttgt tggcctggcg ccacacggtt tcgctctggg
     2821 gctccacgcc gcccacggca cagaagagcg ccacggcacc gtccagcacc cgcaggctgc
     2881 gctccacctc cacagtgaag tccacgtggc ccggggtatc gatcaggttg atcttgtacg
     2941 tgtcgccacg gtatttccaa ctggtggtgg tggcggcgct ggtg
//
LOCUS       DAOQOC010000253         2982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17473_length_2982_cov_3.423984, whole genome shotgun sequence.
ACCESSION   DAOQOC010000253 DAOQOC010000000
VERSION     DAOQOC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17473_length_2982_cov_3.423984"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PL106_05195"
     CDS             complement(<1..1013)
                     /locus_tag="PL106_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNA32496.1"
                     /translation="MPTRLRAGSFTGMIDLRSDTVTRPTPAMLQAMANAPTGDDVFGE
                     DPTVNELERRMAALFGHEAALFCSSGTQTNQIAINVHTRPGDEVLCDEGAHVYRYEGG
                     GTMANSGCSVRFLPAERGRFTARDIEAAINDPGNPHLARTRLVVIENTANRGGGAVWD
                     IVEAGRVREVCDAHGLALHLDGARIFNAMAVDRTAPSHWGTTFHSVSICLSKGLGAPV
                     GSVLTGNAALIAQARRVRKRWGGGMRQAGILAAAGLHALDHHVDRLHEDHERAKRLER
                     ILFDLPWVEHVLPVETNIVVVALKDSGGVPRVLAALKEREVLAVAFGPGMLRLVTHLD
                     TDD"
     gene            complement(1052..2230)
                     /locus_tag="PL106_05200"
     CDS             complement(1052..2230)
                     /locus_tag="PL106_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNA32497.1"
                     /translation="MRHLWSSTMLLTLAASGQVWTPLPDFPGTPRDDAASFSTIAQVY
                     VGTGMDAGFQLTNDWYVYNVGDGWQAIAPLPANGRQYASGFTLTGLGPNGGYLFGGLD
                     ANGPLDELWQYNAQLDEWTPLAPLPAPGRYACAVIATTDFAYVCGGLLAGGVPTNEVW
                     RYDRATDSWSPRADLPGPARHRAAAMDLMVIGGADSSYQALSDVYRYDPDADQWEQEL
                     DLPAPRYGAKCAGSYLLCGASSTSQFHNDVWSYEVIDWDATLVPPFTGGPRKGGVAAY
                     VWPTSESDAILFGLGTDGNSRFNDWWMVEFYTGVEEHHGATTTVFPNPASTELTPVRP
                     ADWMSGSFAIHDATGRKVLGGTLQREVPLDVRCLAPGRYGLRMDHEGRTLRAFFIKLP
                     "
     gene            2324..2731
                     /locus_tag="PL106_05205"
     CDS             2324..2731
                     /locus_tag="PL106_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agenet domain-containing protein"
                     /protein_id="HNA32498.1"
                     /translation="MRHLSLLATALLFCATVANAQNVNDAVEIESAGSWYPGKVLKLE
                     GDKFFVSYDGWSESFNEWVTKERLRTGKTGTAPATPAALTKFKVGDRVEVEYGMAPAP
                     ATIVYVGENKYEVEFDYKPWGKKFYTEKEIKKL"
BASE COUNT          575 a          920 c          947 g          540 t
ORIGIN      
        1 tcatcatccg tatcgaggtg cgtcaccaac cggagcatgc cggggccgaa ggccaccgcg
       61 agcacctcgc gctctttcaa tgcggcaagt acgcgaggta cgccaccgct atctttcagg
      121 gccaccacca cgatgttcgt ctcaaccggg agcacatgtt ccacccaggg caggtcgaac
      181 aggatgcgct ccagccgctt cgcccgctca tggtcctcat gcaggcggtc cacatggtga
      241 tcgagcgcgt ggaggcccgc tgccgcaagg atcccggcct gccgcatgcc gccaccccaa
      301 cgtttgcgca ctcggcgtgc ttgcgcgatg agcgccgcgt tcccggtgag cacgctgccc
      361 accggtgcac cgaggccctt gctcaggcag atggataccg aatggaatgt ggtgccccag
      421 tgcgaagggg ccgtcctgtc cacggccatg gcattgaaga tgcgcgcgcc atcgaggtgc
      481 aacgcgagcc cgtgcgcatc acaaacctcg cgtacgcgtc ccgcttccac gatatcccag
      541 acagccccac ctccccgatt cgcggtattt tcgatcacga ccagccgggt gcgtgccaga
      601 tgggggttgc cggggtcgtt gatcgccgct tcaatatccc gggccgtgaa tcggccccgc
      661 tcggcgggaa ggaacctcac actgcatccg ctgttggcca tggtcccgcc accttcgtac
      721 cggtacacgt gcgcgccttc atcgcacagc acttcgtcgc ccggacgtgt atgcacgttg
      781 atcgcgatct ggttggtctg tgtgccgctg gaacagaaga gcgccgcctc gtgcccgaag
      841 agcgctgcca tgcgccgctc gagttcattc accgtggggt cctcgccgaa cacatcatcg
      901 ccggtaggcg cattggccat ggcttgcaac atggcgggcg taggccgggt cacggtgtca
      961 gaacgcagat cgatcatgcc ggtgaaggat cctgcccgga ggcgggtggg caagatagcc
     1021 agtgcttcac gcgcttgagc cgtgctccgg ttcaggggag cttgatgaag aaggcccgta
     1081 gcgttcgacc ctcatggtcc atgcgtagcc cgtaacgtcc gggagccagg cagcgtacgt
     1141 ccagtggaac ctcgcgttgc agggtgccac caaggacttt gcgccctgtg gcatcgtgga
     1201 tcgcgaatga cccgctcatc cagtccgctg gtcgtacagg tgtcagttcc gtggaagcag
     1261 ggttggggaa gaccgttgtt gttgccccgt gatgctcctc cacaccggtg tagaattcga
     1321 ccatccacca atcgttaaac cgcgaattgc catcggtgcc caggccgaag aggatggcat
     1381 cggactccga ggtgggccac acgtatgcgg ctacaccgcc cttgcgcggc ccccccgtga
     1441 acggtggaac aagcgtggcg tcccaatcga tcacttcgta ggaccataca tcgttgtgga
     1501 attgcgatgt ggaactggcc ccgcacagca ggtaggatcc cgcgcatttg gcaccataac
     1561 gcggtgcggg cagatcgagc tcctgctccc actggtcggc atcgggatcg taccggtaca
     1621 catcgctcag ggcctggtag gaactgtcgg cgccaccgat caccatcagg tccatggcgg
     1681 ccgcccgatg ccgggccggg ccagggagat ccgccctcgg cgaccaactg tccgtggccc
     1741 ggtcatagcg ccagacctcg ttggtgggaa cgccaccggc gagcaagccg ccgcacacgt
     1801 acgcgaagtc cgtcgtagcg atcaccgcgc atgcgtagcg tcccggtgcc gggagcgggg
     1861 ccagcggggt ccattcatcc aattgggcgt tgtattgcca cagctcgtcc agcggtccgt
     1921 tcgcgtcgag ccctccgaag aggtagcccc cgttcggccc caaacctgta agcgtgaatc
     1981 cgctggcgta ttgcctgcca ttggccggca gcggagcgat cgcctgccaa ccgtcgccca
     2041 cgttgtaaac ataccagtcg ttggtcagtt ggaaaccagc gtccatgcct gttcccacgt
     2101 acacttgggc aatggtcgag aacgaggccg catcgtcccg gggtgtaccg gggaaatcag
     2161 gcagtggggt ccaaacctgg ccggaggcgg cgagggtcag cagcatggtg gaactccaga
     2221 ggtgtcgcat cctgtgaagg tagggatgcg ggcgctttcc acggtggacc gtatcttggc
     2281 acggtcttcg tccagcgaag atccggacat aacaaaactc cacatgagac atctctccct
     2341 tttggcaaca gccttgcttt tctgtgccac cgtcgcgaac gcccagaacg tgaatgacgc
     2401 cgtcgagatc gaatccgctg gatcctggta tcccggtaag gtactgaagt tggaaggtga
     2461 caagttcttc gtgtcctacg atggttggag cgaatccttc aacgagtggg tgaccaagga
     2521 acggttgcgg acgggcaaga ccgggaccgc acctgccacc cccgctgccc tcaccaagtt
     2581 caaggtgggt gatcgcgtag aggtggagta cggcatggcg cctgctccgg ccactatcgt
     2641 gtacgttggc gagaacaagt acgaagtgga attcgactac aagccctggg gcaagaaatt
     2701 ctacacggag aaagagatca agaaactctg atcactggaa cgcaacgtgc gagggtcgct
     2761 cctgaccggg gcgaccttcg ttgttcaaag atcggatcaa gggagctggc tcaacgtgac
     2821 aaggggaggg tagggaagct cctcggtagg ctcggagtga caaggggtgg agagtaggaa
     2881 agggctacgg caggctcaac gtgacaaggg gagggttggg aagcgctcct cgcttcgccg
     2941 tagcggctac gcaaaggcgc agtcggcagg ctcagcgtga ca
//
LOCUS       DAOQOC010000254         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17666_length_2965_cov_3.293471, whole genome shotgun sequence.
ACCESSION   DAOQOC010000254 DAOQOC010000000
VERSION     DAOQOC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17666_length_2965_cov_3.293471"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PL106_05210"
     CDS             <1..175
                     /locus_tag="PL106_05210"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551699.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HNA32499.1"
                     /translation="SGAGDLAEAVRTAVINKRAGGQGLISGRKAFQRPMKEGVALLNA
                     IQDVYLNKEVTVA"
     gene            267..1112
                     /gene="accD"
                     /locus_tag="PL106_05215"
     CDS             267..1112
                     /gene="accD"
                     /locus_tag="PL106_05215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNA32500.1"
                     /translation="MGWFTRVKEGITTSTEDKKETPEGLWYKCPECSEIATTEDHENN
                     LWVCAKCGHHEKIGSAEYFALLFDDQKYKEIGADLAAGDPLHFEDTKKYVDRLAKTRK
                     DTGLNDALRAAEGKLDKYPVVIACMDFRFIGGSMGSVVGEKIALAADTALRKKCPFII
                     ISKSGGARMMEAGFSLMQMAKTSAKLTLLAKKRIPYISVITDPTTGGVTASFAMLGDV
                     IIAEPKALVAFAGPRVVRETIGRDLPEGFQTSEFVLEHGFLDKIVERKDLKHPLARFI
                     QFFQN"
     gene            1510..1779
                     /gene="rpsO"
                     /locus_tag="PL106_05220"
     CDS             1510..1779
                     /gene="rpsO"
                     /locus_tag="PL106_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNA32501.1"
                     /translation="MNLTKEAKAEIFKTFGGSEKNTGSTEGQIALFTKRIEHLTGHLK
                     TNKKDHVTESALLDLVGKRKQLLNYLKTYDIERYRAIIGKLGLRK"
     gene            1931..>2965
                     /locus_tag="PL106_05225"
     CDS             1931..>2965
                     /locus_tag="PL106_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNA32502.1"
                     /translation="MRPQGIQKSIALGDGRTISIETGVLAKQADGAVTVRIGDTILLA
                     TVVATKEAREGIDFLPLTVEYREKFSAAGRYPGGFFKRENRPSDGEVLVSRLIDRALR
                     PLFNDDFHGDTQVMVSLLSTDKKNPSDSIACLAAAAALAVSDIPFNGPVSEVRVSRKN
                     GTWSINPTFDAIENADIDLIVAGTSSDILMVEGEMKEVSEAEMIEGIKLAHDAIKLQC
                     QALNELAAAVPKSSPKRTYNHEEKDEALEKAIYNATFQKYYDIASVASAKEVRSEAFA
                     KVKEEFVATLPEETKAKKAMLARYFKKAQKAGVRRVVLEKGVRLDGRKTTDIRPIWSE
                     VDVLPGTHGSA"
BASE COUNT          697 a          943 c          837 g          488 t
ORIGIN      
        1 cagtggcgca ggtgatctgg ccgaggccgt gcgcaccgcc gtgatcaaca aacgcgccgg
       61 tggtcagggg ctcatcagcg gtcggaaagc gttccagcgt ccgatgaagg agggtgtggc
      121 cctgctcaat gccattcagg acgtttacct caacaaagag gtgaccgtcg cttgatcttc
      181 agccgggtgt gatgtgatct ccccgaggtc atcatctctt cccagcgcct gcaccacacg
      241 acccgaacac caatccaact tcggccatgg gctggttcac acgcgtcaag gagggcatca
      301 ccacctccac cgaggacaag aaggagactc ccgaaggctt gtggtacaaa tgccccgagt
      361 gctccgagat cgccaccacc gaggaccacg agaacaacct ctgggtctgt gccaagtgcg
      421 gccatcacga aaagatcggt agcgccgagt atttcgcgct cctcttcgac gaccagaaat
      481 acaaggagat cggcgcggat ctcgctgcgg gcgacccgct gcatttcgag gacacgaaga
      541 agtacgtgga ccgcctggcg aagacgcgca aggacaccgg cctgaacgat gccctgcgcg
      601 ccgcagaagg caagctggac aagtatcccg tggtgatcgc ttgcatggat ttccggttca
      661 tcggtggaag catgggcagt gtggtcggcg agaagatcgc cctggccgcc gataccgcgc
      721 tgcgcaagaa atgccccttc atcatcatca gtaaaagtgg tggagcacgc atgatggaag
      781 ccgggttcag cctgatgcag atggccaaga ccagcgccaa actcacgctc ctggccaaaa
      841 agcgcatccc gtacatcagt gtgatcaccg acccgaccac gggtggtgta acggccagct
      901 tcgccatgct gggcgatgtg atcattgccg aaccgaaggc cctcgtggcc ttcgccggac
      961 cacgcgttgt gcgcgaaacc atcggccgcg acctgcccga aggcttccag accagtgagt
     1021 tcgtgctgga acacggcttc ttggacaaga tcgtggagcg taaggatctg aaacacccgc
     1081 tggcccgatt catccagttc ttccagaatt gaacccgcca cgcggatgtt ccccgcagcg
     1141 caggcccgtt cctttggtca ggtggtggcg gtgatgagcg gttccgtacg attcaaatgc
     1201 ttggctggcc aagcaatcat gtctaatgac gtgattatca gaactatgcg catatatcag
     1261 caccaaatca aggaacacaa tgatgcttct acgcgccgta aacgcataaa aatgcgtgaa
     1321 tgcttgtttg gccagccaag caaagtaatg acccgccgat ccccaaccac ccctcaagtc
     1381 ctcactccgg ctccccctca ccctgcttca agccccgctt cattcctgat cgctcccacc
     1441 gatcgatgaa atgtctacct tcgcgccccg gctcgccaaa ggggtgtgcc cggagaccac
     1501 gaaagaacca tgaacctgac caaagaggcc aaagccgaga tcttcaaaac cttcggtggt
     1561 agcgagaaga acaccggcag caccgagggc cagatcgcgc tcttcaccaa gcgcatcgag
     1621 cacctgaccg ggcacctgaa gaccaacaag aaagaccacg tcaccgagtc ggccctgttg
     1681 gacctcgtgg gcaagcgcaa gcaactgctc aactacctca agacctacga catcgagcgc
     1741 taccgtgcga tcatcggcaa gctgggtctg cgcaaataag atccctgccg ttcgcgcggg
     1801 cggtcttcga tcctgaatgg acgacggggc gatcgtgaag aacgatcgcc cttttttctg
     1861 accatcggga acgcctcctc aagaagaaca ccaaccaaca caacggcacg aagggcgtgc
     1921 ccatacagac atgagaccac aaggcatcca aaagtccatc gccctgggcg acggccgcac
     1981 catctccatc gagacgggcg tactggccaa gcaggccgac ggcgccgtca ccgtgcgcat
     2041 cggcgatacc atcctcctgg ccaccgtggt ggccaccaaa gaggcccgcg aaggcatcga
     2101 tttcctcccg ctcacggtgg agtaccgcga gaaattcagt gccgccggcc ggtatccggg
     2161 cggcttcttc aaacgcgaga accggcccag cgatggtgag gtgctcgtca gccgactgat
     2221 cgaccgcgcc ctgcgccccc tgttcaacga cgacttccat ggtgataccc aggtgatggt
     2281 atcactgttg agcaccgaca agaagaaccc cagcgacagc atcgcatgcc tggcggccgc
     2341 cgcagcactc gccgtgagcg acatcccctt caacgggcca gtgagcgagg tacgcgtctc
     2401 ccgcaagaac ggtacgtgga gcatcaaccc cactttcgat gcgatcgaga acgccgacat
     2461 cgacctgatc gtggccggta cctccagcga tatcctcatg gtggagggcg aaatgaagga
     2521 agtgagcgag gccgagatga tcgagggcat caaactcgcc cacgatgcca tcaaactcca
     2581 gtgccaggca ctgaacgagc tggccgctgc cgttcccaaa agcagcccca agcggaccta
     2641 caaccacgag gaaaaggacg aggccctgga gaaggccatt tacaacgcca ccttccagaa
     2701 gtactacgac atcgcctctg tggccagtgc caaagaagtg cgcagcgagg cgttcgccaa
     2761 ggtgaaggag gagttcgtcg ccactctgcc cgaggagacc aaggccaaga aggccatgct
     2821 cgcccgctac ttcaaaaagg cccagaaagc cggtgtgcgc cgtgtggtcc tggagaaagg
     2881 tgttcgtctc gatggccgga aaaccaccga catccgcccc atctggagcg aggtggacgt
     2941 gctgcccggc acccacggca gcgcc
//
LOCUS       DAOQOC010000255         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17700_length_2962_cov_3.086343, whole genome shotgun sequence.
ACCESSION   DAOQOC010000255 DAOQOC010000000
VERSION     DAOQOC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17700_length_2962_cov_3.086343"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="PL106_05230"
     CDS             <1..1544
                     /locus_tag="PL106_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32503.1"
                     /translation="RTYRPESTVAAATERCNPSRVRGTGTTFQTIPSAGVKLWVSVNI
                     NCAHCHNAQTGRGELPGNGNVAGTGAGTLVDFTPNRNMAADLRSVYRKVGFFYNTTEC
                     NAGFGMLSEGVMETWFNQGGTGNYLGDYEPELLSWSGGITQTNCVACFNTNSFPLAST
                     TVQDAMPAVGLRETINGASIGSTARLNTMKALVDEAAHSSEYGMIVKGMYGGEMRGFY
                     YTGSNNYQSDITGQTVTHTQLVNSAQGSGGPLSWTLVVPQTKVRLGVDADADGILDHD
                     DLNARVNVRAVLEGPFDGTAMKSDLNDADLLPATDPFGLGETMNPAVKSFTGLSTPVD
                     WARVELRSNADPAVVVAQRAVLVQRSGNLMQPTGEQIITFPGVAAGNYHVAVRHRNHL
                     GIMSLSPYMLRDANTLVDFTTAATATYGTNARKTVGGTMVMWMGDVTGNGTIKYTGSG
                     NDRDPVLVKVGSTAPNATMNGYWPEDINLNGTVKYTGSANDRDPILQNVGATVPTNSR
                     TEQLP"
     gene            1741..2169
                     /gene="rplM"
                     /locus_tag="PL106_05235"
     CDS             1741..2169
                     /gene="rplM"
                     /locus_tag="PL106_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNA32504.1"
                     /translation="MANKATVQKEWLLVDAENEVLGRVASKIAMLVRGKHKPTFTPHV
                     DTGDHVVVINADKVRLTGAKWEDKTYLRYSLYPGGQRAETAKALLRRKPHSVLEEAVR
                     GMLPKTRLGEAQFGNLHVNVGGTHKHEAQKPRKIDLNSIK"
     gene            2171..2557
                     /gene="rpsI"
                     /locus_tag="PL106_05240"
     CDS             2171..2557
                     /gene="rpsI"
                     /locus_tag="PL106_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNA32505.1"
                     /translation="MASVNSLGRRKSSVARVILSDGNGDIKVNGVEGKEYFPITMQQF
                     KLMQPFKLTETEGKYDVTIRVDGGGITGQVEAVRLGIARALVKLDPEHKPKLKAEDLM
                     TRNPREVERKKFGRKKARKRYQFSKR"
     gene            2729..>2962
                     /gene="rpsB"
                     /locus_tag="PL106_05245"
     CDS             2729..>2962
                     /gene="rpsB"
                     /locus_tag="PL106_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNA32506.1"
                     /translation="MARTDFKQLLDAGVHFGHLRRKWNPNMAPYIFGEKKGIHIIDLN
                     KTAVKLDEAANAMKNIARSGKKVLFVATKKQAKD"
BASE COUNT          660 a          937 c          938 g          427 t
ORIGIN      
        1 accgcacgta ccggccggag agcaccgtgg ccgccgccac cgagcgttgc aacccgagca
       61 gggtgcgtgg tacgggaacc accttccaaa cgataccaag tgccggtgtg aaattgtggg
      121 tgagtgtgaa catcaactgc gcgcactgcc acaacgcgca gaccggtagg ggcgaactgc
      181 ccgggaacgg gaatgtcgcg ggcacgggtg cgggcacctt ggtcgatttc acccccaacc
      241 ggaacatggc ggcggacctg cgcagcgtgt accggaaggt cggtttcttt tacaacacca
      301 cggaatgcaa cgcgggcttc ggcatgctga gcgagggcgt gatggagacc tggttcaacc
      361 agggtggcac gggcaactac ctgggcgact acgagcccga actcctctcg tggtccggcg
      421 gcatcaccca gaccaactgc gtggcgtgct tcaacaccaa cagcttcccg ctggccagca
      481 ccaccgtgca ggacgccatg cccgccgtgg gcctgcgtga aacgatcaac ggcgccagca
      541 taggcagtac ggcccgcttg aacaccatga aggccctggt ggacgaggcc gcgcacagca
      601 gcgaatacgg catgatcgtg aagggcatgt acggcggcga gatgcgcggc ttctactaca
      661 cgggtagtaa caactaccag agcgacatca ccgggcagac cgtcacccac acgcaactgg
      721 tgaacagtgc acagggcagt ggcggaccgc tcagttggac cctggtggtg ccgcaaacga
      781 aggtgcgcct gggtgtggat gcggacgccg acggcattct cgaccacgac gacctgaacg
      841 cccgcgtgaa cgtgcgtgcc gtgctggaag gacccttcga cggcacggcc atgaagagcg
      901 acctgaacga tgcggacctg ttgcccgcca ccgatccctt cggcctgggc gaaacgatga
      961 acccggcggt gaagagtttc accggccttt cgaccccggt ggattgggcg cgcgtggaac
     1021 tgcgcagcaa cgccgacccc gccgtggtgg tggcacagcg ggccgtgctg gtacagcgct
     1081 cgggcaacct gatgcaaccc accggcgagc agatcatcac cttccccggc gtggcggcgg
     1141 gcaactacca cgtggccgtg cggcaccgca atcacttggg catcatgagc ctgtcgccct
     1201 acatgctgcg cgatgcgaac actttggtgg acttcaccac ggccgccacg gccacctacg
     1261 gcaccaacgc gcgcaagacc gttggtggca ccatggtcat gtggatgggc gatgtgaccg
     1321 ggaatggcac catcaaatac accggcagtg gtaacgaccg cgatccggtg ctggtgaagg
     1381 tgggcagcac cgcgcccaat gccacgatga acggctactg gcccgaggac atcaacctga
     1441 acggcacggt gaaatacacg ggcagcgcga acgaccggga ccccatcctg cagaacgtgg
     1501 gggccaccgt cccgacgaac tcgcgtaccg agcagctgcc ctgatggctt gcggctgaac
     1561 caccccgccc cggaggggtg tgtaaaggat ttctccacgg ctttttgtgg gatcgggccg
     1621 tcgatctatc ttcgcgcccc gttttacccg ccatagcccc gctcaggggt gggggcgggt
     1681 gcggggcgaa cagacaaaaa acccaggcgt accgtggctt ccacgaccct catcacctcc
     1741 atggccaaca aggccaccgt gcagaaagaa tggctgctgg tggacgctga gaacgaggtt
     1801 ctcggccgcg tggccagcaa gatcgccatg ctggtgcgtg gcaagcacaa gcccaccttc
     1861 accccgcacg tcgacaccgg cgaccatgtg gtggtgatca atgccgacaa ggttcgtctc
     1921 accggcgcca agtgggagga caagacctac ctgcgctaca gcctgtaccc gggcggccag
     1981 cgcgccgaaa cggccaaggc cctgctgcgc cgcaagccgc acagcgtatt ggaggaggcc
     2041 gtgcgcggca tgctccccaa gacgcgcttg ggcgaagcgc aattcggcaa cctgcacgtg
     2101 aacgtgggcg gaacccacaa gcacgaagcg cagaagcccc gcaagatcga cctcaacagc
     2161 atcaagtaag atggcatcag tgaacagcct gggccgccgc aagagcagtg tggcgcgcgt
     2221 gatcctcagc gatggcaacg gggatatcaa ggtgaacggc gtggaaggca aggagtactt
     2281 ccccatcacc atgcagcagt tcaagctgat gcagcctttc aaactcaccg aaaccgaggg
     2341 caagtacgac gtcaccatca gggtggacgg cggcggcatc accggccagg tggaagccgt
     2401 gcgtctgggc atcgcccgtg cgctggtgaa gctcgatccc gagcacaagc ccaagctcaa
     2461 ggccgaggac ctcatgacgc gtaatccccg cgaggtggag cgcaagaagt tcggacgcaa
     2521 gaaggcgcgc aagcgctacc agttcagcaa gcgttaagtc ccacgcttgc gtgcccaccc
     2581 aagtccgccg cagtgcggac aaaggagggt tcagcatcca atccctccgg actgttgttg
     2641 tcacgctgag cttgtcgaag cgcgacaaca cgctaccggg gagatgcctg cgaaacggga
     2701 acaaggaaag tgaaccaaaa gaaggaacat ggcacgtact gatttcaagc aactcctcga
     2761 cgccggcgtc cacttcggcc acctgcgccg caagtggaac cccaacatgg cgccctacat
     2821 cttcggcgag aagaagggca tccacatcat cgacctgaac aagacggcgg tgaagttgga
     2881 cgaggccgcc aacgcgatga agaacatcgc ccgcagcggc aagaaagtgc tcttcgtggc
     2941 aacgaagaag caggccaagg ac
//
LOCUS       DAOQOC010000256         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17730_length_2960_cov_3.254733, whole genome shotgun sequence.
ACCESSION   DAOQOC010000256 DAOQOC010000000
VERSION     DAOQOC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17730_length_2960_cov_3.254733"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..467
                     /locus_tag="PL106_05250"
     CDS             168..467
                     /locus_tag="PL106_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32507.1"
                     /translation="MSDLAPRLRQVHARVEGLLKDRDRSASEHLGLVEQLRELKRSDE
                     VLRARIEELEREIAVLRAVQTVPHGNGATGDRSGTKERIDELVSEIDRCLALLKN"
     gene            485..766
                     /locus_tag="PL106_05255"
     CDS             485..766
                     /locus_tag="PL106_05255"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNA32508.1"
                     /translation="MNETSIRIELAGRAYPLTVLPEEEANIRKAAAEINESIARLKAS
                     YPMTDKQDLLAMAALEVTTRAFNQATAQARAEDIAAVEDLERLLSSIRS"
     gene            871..2415
                     /gene="rny"
                     /locus_tag="PL106_05260"
     CDS             871..2415
                     /gene="rny"
                     /locus_tag="PL106_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNA32509.1"
                     /translation="MDSTIMTLIGAVVGLIAGGGIVYVLLNNLMTKRRDGILKEAEAE
                     GEALKKQKLAEAKEKFLQLREEHEKEAREKDKRANQALEKSKQREQQLSRQQQELGTK
                     EKQLDHLKSELTTRLQGLERRAEDVEKNHAKQVQLLEKISGLNAEEAKKQLMDSLRDE
                     ARTAAMAHIKEVTEEAKITANKEAKRIIIQTIQRTATEHAVENAVSVFNIENDDVKGR
                     IIGREGRNIRTLEEATGVEIVVDDPPGAIILSCFDPVRREMARLALHQLVKDGRIHPG
                     RIEEIVAKTKRHLEEEIVEIGKRTCIDLGIHGLHNELIRMVGRMRYRSSYGQNLLQHS
                     REVANLSATMAAELGLNVKAAKRAGLLHDIGKVPDEAPELPHAILGMQLAEKYGEKPD
                     VLNAIGAHHDEIEMTTLISPIVQACDAISGARPGARREAMEQYIQRLKDLEGLAMSYQ
                     GVQKAFAIQAGRELRVMVESEKVTDAQADTISMSLAERIQNEMTYPGQVKITVIRERR
                     STAVAK"
     gene            2432..>2960
                     /locus_tag="PL106_05265"
     CDS             2432..>2960
                     /locus_tag="PL106_05265"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA32510.1"
                     /translation="MPKGTILVTGGAGFIGSHVCDALLAHGYAVRCFDNLSTGKAANV
                     SHLEYDPGFSFVKGDLGVPRELAAAMDGVSAIVHLAALGSVPRSIADPLASEAANLAG
                     FLHLLEAARDARVDRIVYASSSSVYGDSQELPKREGREGRPLSPYAVTKAMDEAYAHL
                     YHSMHGMRIIGLRFFN"
BASE COUNT          671 a          889 c          939 g          461 t
ORIGIN      
        1 gatcaagacc cgcccgggcg tgcgatcccg agcacgcgca tggatcggat accgggtcgt
       61 tgggccactc catcaacaga tgcgcgtgat aactccattc attcccttca atcccatggt
      121 cttatattcg tcccggaccc tgaaaacgga ctcctggatc cttagccgtg agcgaccttg
      181 ccccccgact tcgccaagtg catgcgcgtg tggaaggtct gctcaaggac cgcgacagga
      241 gcgcatcgga acacctcggg cttgtagagc aactgcgtga gttgaagcgc agtgatgagg
      301 tactccgggc gcgtatcgag gaactggaac gggagatcgc ggtcctccgc gcggttcaaa
      361 cggtacccca tgggaatggg gccaccgggg atcgctcggg aacgaaggaa cggatcgatg
      421 aattggtgag cgagatcgat cgctgcctgg ccttattgaa aaactgacca gcacgatcac
      481 cgccatgaac gaaacatcga tccggatcga gctggccggc cgggcctatc cgctgacggt
      541 attaccggag gaggaagcca acatccgcaa ggcggccgcc gagatcaatg agagcatcgc
      601 ccgcctgaag gcatcgtatc cgatgaccga caagcaggat ctgttggcca tggccgcgct
      661 ggaagtgacc acacgcgcgt tcaatcaggc caccgcacag gcccgcgccg aggatatcgc
      721 ggccgtggag gatctggagc ggctgctttc ctcgatccgg agctgatccc cttcgctccc
      781 cggagtgccc agggccgtga atatgaacac ggatccatcc gcatccatct gcgggtgagg
      841 tccataaaac atcgcctggg cgaacaacga atggattcga ccatcatgac actcatcggg
      901 gccgtggtcg gcctcatcgc aggaggcggc atcgtctacg tcctgttgaa caacctgatg
      961 accaaacgcc gggacggcat cctgaaggag gcggaggccg aaggagaagc cctgaagaag
     1021 cagaagctcg ccgaggccaa ggagaagttc ctgcaactgc gcgaagagca cgagaaggaa
     1081 gcccgcgaaa aggacaaacg ggcgaaccag gccctggaga aaagcaaaca gcgcgaacaa
     1141 caactcagtc ggcaacaaca ggaactcggc accaaggaga agcagctcga ccacttgaaa
     1201 tcggagctta ccacccgcct ccaaggtctg gaacgccgcg ccgaggacgt ggagaagaac
     1261 cacgccaagc aggtgcaact gctggagaag atcagcgggc tgaacgccga agaggccaag
     1321 aaacagttga tggacagctt gcgcgatgag gcccgcaccg ccgccatggc ccacatcaag
     1381 gaggttaccg aggaggcgaa gatcacggcc aacaaggagg ccaaacgcat catcatccag
     1441 accatccagc gcaccgccac cgagcacgcc gtggagaacg ccgtcagcgt gttcaacatc
     1501 gagaacgacg atgtgaaagg ccggatcatc ggccgcgaag gccgcaacat ccgcaccttg
     1561 gaagaagcaa ccggcgtgga gatcgtggtg gatgaccccc cgggagccat cattctgagc
     1621 tgtttcgacc cggtgcgccg cgaaatggcc cgcttggcct tgcaccagtt ggtgaaggac
     1681 ggtcgcatcc acccgggccg tattgaggag atcgtggcca agaccaagcg ccatctggaa
     1741 gaggagatcg tggagatcgg caaacgcacc tgtatcgacc tggggatcca cgggctgcac
     1801 aacgaactga tccgtatggt gggccgcatg cgctatcgca gcagctacgg gcagaacctg
     1861 ttgcagcata gccgcgaggt ggcgaacctg agcgccacca tggccgctga actcggcctg
     1921 aacgtgaagg cggccaaacg cgcgggcctg ctgcacgaca tcggcaaggt gcctgatgag
     1981 gcgccggagt tgccgcacgc catcctgggt atgcaactgg cggagaagta cggcgagaaa
     2041 cccgacgtgc tgaacgccat cggtgcgcac cacgacgaga tcgagatgac cacgttgatc
     2101 agtccgatcg tgcaggcctg tgatgccatc agcggtgccc gcccgggcgc ccgtcgcgag
     2161 gccatggagc agtacatcca gcgcctgaag gatctggaag gactggccat gagctaccag
     2221 ggcgtgcaaa aagcgttcgc catccaggcc ggccgggaat tgcgcgtgat ggtggagagc
     2281 gagaaggtga ccgatgccca ggccgatacg atcagcatga gtctggccga acgtatccag
     2341 aacgagatga cctacccggg ccaggtcaag atcaccgtga tcagggagcg caggagcacg
     2401 gctgtggcca agtaggacga aagcggcccc gatgcccaag ggaaccattc ttgttaccgg
     2461 tggtgccggt ttcatcggct cgcatgtctg cgatgcgctg ctcgcccacg gatatgccgt
     2521 tcgctgtttc gacaaccttt cgaccgggaa ggcggccaac gtatcgcact tggaatatga
     2581 tcccggtttc tcgttcgtga agggtgacct cggcgttcct cgggagctgg ctgccgccat
     2641 ggacggggtc tcggccatcg tgcatctggc cgcattgggc agtgtgccgc gcagcatcgc
     2701 cgatccactg gccagcgagg cggccaacct tgccggtttc ctgcatctac tggaggcggc
     2761 acgtgatgcg cgtgtggacc ggatcgtcta cgccagcagc agcagcgtgt acggcgacag
     2821 ccaggaactg cccaagcgcg aaggccgcga gggccgcccg ctttcgcctt acgcggtgac
     2881 caaggcgatg gacgaggcct atgcgcatct ctaccactcc atgcacggca tgcggatcat
     2941 cggtctccgg ttcttcaatg
//
LOCUS       DAOQOC010000257         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17858_length_2948_cov_5.175596, whole genome shotgun sequence.
ACCESSION   DAOQOC010000257 DAOQOC010000000
VERSION     DAOQOC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17858_length_2948_cov_5.175596"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="PL106_05270"
     CDS             complement(<1..1676)
                     /locus_tag="PL106_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNA32511.1"
                     /translation="MRSSWLIVGSILLLTSAAQAQFGPAYMYLCEGPRSVDLADVDVD
                     GDLDMIVGSRQGLSLHVNLDGLGTLDEPLTLVQEETIACTGDLNGDGAIDIVGSRDDG
                     TGISVHFNNGQGGFPSSVVLSETLSADEMHAADLDDDGDTDLFFALPGGQLALSYNNG
                     YGEFSAINVLATVPQLGLIRAVDADADGDTDLICSSRITNQVFISSNIAGELQTPEPV
                     SLSGFGLVKDLDNDGRPDLLMANVAAGIVGWQRNEPDGQTFDAPTVMDASVNAPEFLG
                     AMDLDGDGDNDAVVTSAGTQEVAWYDNTDGLGSFGPRQTVATSLPATGLAAGDIDNDG
                     DEDLFVASAVLDKVVRFTNMSSSIGTISGRVFNDLNGDGIFNGTDHGLANMPVEAVGL
                     GVTYTNVSGLYWFNSAPDEHQVSKPGEVGWSFTTPAAYDVIVPAQGASQGNDFGLRAP
                     SPVVALTPDLGSAPILCNQSVSYWVGVENTGTSSADTRVALDLDDRSTFEWASPAPDS
                     VQNGVVVWRFEGLQPTQRRDVHIVVHMAGSDFVGDTLHDVMQVVTLINGVP"
     gene            complement(1782..>2948)
                     /locus_tag="PL106_05275"
     CDS             complement(1782..>2948)
                     /locus_tag="PL106_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNA32512.1"
                     /translation="KLDQGATVGDALRLMAEHRIGGIPVVDQAGKLVGIVTNRDLRFE
                     KSMGRAVAEVMTKDRIITAKPETSMSEAEGVLQEYKIEKLPVVDQRGQLVGLITYKDI
                     LKLKQHPVACKDKLGRLRVAAAIGVAGDSMERAAALVEAGVDAVVIDTAHGHTKGVID
                     MLRQVKTAFRTIDVVVGNIATAAAARALVEAGADAVKVGIGPGSICTTRVIAGVGVPQ
                     LTAVHDCASAIEGSGVPVIADGGIRYTGDAVKALAAGAGTVMIGSMFAGVEESPGETI
                     IYEGRRFKAYRGMGSLEAMQHGSKDRYFQDMEDDVKKLVPEGISGRVPYKGHLHEVTH
                     QLTGGLRAGMGYCGAADLEALRRAQFIRITSAGMKESHPHDVFITREAPNYSLR"
BASE COUNT          550 a          954 c          890 g          554 t
ORIGIN      
        1 ggtacgccat tgatcagcgt cacgacctgc atgacatcat ggagcgtatc gcccacgaaa
       61 tcgctgccgg ccatgtgtac cacgatgtgg acatcgcggc gctgggtggg ttgcaggcct
      121 tcaaagcgcc agaccaccac accgttctgc accgagtcgg gtgcgggcga tgcccactcg
      181 aaggtggaac ggtcatcgag atcgagggcg acacgcgtgt ccgcggatga ggtcccggta
      241 ttctccacac cgacccagta ggacacggac tggttgcaca gtatcggggc gctgcccagg
      301 tcgggcgtca acgcgactac ggggctgggc gcccgcaggc cgaagtcgtt cccttgagaa
      361 gcgccttggg cgggcacgat cacatcgtac gcagcgggcg tggtgaacga ccagcccacc
      421 tcgccgggtt tgctcacctg atgttcgtcg ggggcggagt tgaaccagta cagaccgctc
      481 acattggtgt acgtgacacc caggccgacg gcttccacgg gcatgttcgc caggccgtga
      541 tcggtaccat tgaagatgcc gtcgccattc aggtcattga acacacggcc gctgatggta
      601 ccgatggaac tggacatatt ggtgaagcgg acgaccttgt cgagcacggc actggccacg
      661 aacaggtcct catcgccatc gttgtcgata tcccctgcgg cgagacccgt ggcgggaagg
      721 ctggtggcca ccgtctggcg agggccaaaa ctccccaggc catcggtatt gtcataccag
      781 gccacttcct gcgtgccggc cgatgtgacc acggcgtcgt tgtccccatc gccgtccaga
      841 tccatcgcgc cgaggaattc gggtgcgttg acggacgcgt ccatcacggt gggcgcatcg
      901 aacgtctgtc catccggttc gttccgttgc cagccgacga tgccggcggc gacgttggcc
      961 atcagcaggt ccggtcgccc atcgttgtcc agatccttca ccagaccgaa accggagagg
     1021 gatacgggct cgggggtttg cagttcacca gcgatgttcg aggagatgaa cacctggttg
     1081 gtaatgcgcg aggagcagat gaggtcggta tccccgtccg catcagcgtc caccgccctg
     1141 atcaggccca gctggggcac cgtggcgagc acgttgatgg ccgagaactc gccgtacccg
     1201 ttgttgtagg agagtgcgag ttgaccgcca ggcagcgcga aaaagagatc ggtatcgcca
     1261 tcatcatcca ggtcggcggc atgcatttca tcggcggaaa gcgtttcgga aagcaccacc
     1321 gaggagggga aacctccctg gccattgttg aagtggacgg agatgcccgt gccatcatcg
     1381 cggctgccca cgatgtcgat ggcaccgtcg ccgttcagat cacccgtgca ggcgatggtc
     1441 tcctcctgaa ccagggtcag cggttcgtcc agggtgccca gtccatcgag gttgacatgg
     1501 aggcttagac cttgtcgcga cccgacgatc atgtccaggt ccccatcaac atccacatcg
     1561 gcgaggtcca ccgatcgcgg gccttcgcac aggtacatgt acgccgggcc gaattgggcc
     1621 tgagcagcgg atgtgagcag aaggatggaa ccaacgatga gccaagagga acgcatgaac
     1681 agggaatttt ggccccctca tacgcgccca gcctcccccg agttgcattt ttccatcgac
     1741 ggaccacccg gacccctcga cgatcaacca gtcagccgat cctaccgaag gctgtaattc
     1801 ggggcctcgc gcgtgatgaa aacatcgtgc ggatggctct ccttcatgcc tgcactggtg
     1861 atccggatga attgggcacg gcgcagtgct tccagatcgg cagcgccaca gtagcccatc
     1921 cctgcgcgca agccgcccgt gagctggtgg gtcacctcgt gcaagtggcc tttgtacggc
     1981 acccggccgc tgatgccttc cggcacaagc ttcttcacat catcctccat gtcctggaag
     2041 tagcggtcct tgctgccgtg ctgcatagcc tccaagctgc ccatgccccg ataggccttg
     2101 aagcggcgcc cttcatagat gatggtttcg cccgggctct cctccacccc ggcgaacatg
     2161 ctgccgatca tcacggtgcc cgcaccggct gccagggcct tcaccgcatc gccggtatag
     2221 cggatgccgc catcagcgat caccggaacc ccgctgccct cgatggcact ggcacagtca
     2281 tgcacggcgg tgagttgcgg taccccgacg cccgcgatca cgcgagtggt gcagatgctg
     2341 cccggtccga tacccacctt taccgcatcg gcaccggctt ccaccaaggc gcgggccgcg
     2401 gcggccgtgg cgatgttgcc caccaccaca tcaatggtcc tgaatgcggt tttgacctgc
     2461 cggagcatgt cgatcacccc tttggtgtgg ccatgtgcgg tatcgatcac cacggcgtca
     2521 acaccggctt ccaccaaggc cgcagcacgc tccatggaat cgcccgcaac accgatcgca
     2581 gcggcaaccc gcaagcggcc gagtttgtcc ttgcaggcta ccggatgctg cttcagcttg
     2641 aggatatcct tgtaggtgat aagacccacg agctggccac gctggtccac aacgggcagt
     2701 ttctcgatct tgtattcctg aaggacccct tcggcttcgg acatgctggt ctccggcttg
     2761 gcggtgatga tgcggtcctt ggtcatcacc tcggccacgg cacggcccat ggatttctcg
     2821 aagcgcagat cgcggttggt aacgatgccc acgagtttac cggcctggtc caccaccggg
     2881 atcccaccga tccggtgctc ggccatcaat cgaagtgcat cgcccaccgt cgccccttgg
     2941 tccagttt
//
LOCUS       DAOQOC010000258         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_17904_length_2944_cov_3.867082, whole genome shotgun sequence.
ACCESSION   DAOQOC010000258 DAOQOC010000000
VERSION     DAOQOC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17904_length_2944_cov_3.867082"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PL106_05280"
     CDS             complement(<1..402)
                     /locus_tag="PL106_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNA32513.1"
                     /translation="MSLDYTAHPTAVIDEGCTIGKGTRIWHFSHIMPGCVIGENCNIG
                     QNVVISPEVKLGRNVKVQNNVSIYTGVECEDDVFLGPSMVFTNVINPRSAVNRRGEYL
                     RTLVKKGASIGANATIVCGHDIGRFAFIGAGA"
     gene            540..1454
                     /locus_tag="PL106_05285"
     CDS             540..1454
                     /locus_tag="PL106_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32514.1"
                     /translation="MRKTLLLGWLLVALAANAQLWCAPGVVWTYRFQDWGGGSSVDYR
                     TEYRYVGDTLYNGMLAHRVDRTSAGVANGQPVGGGTSPTYEAEADDVVWVWTTGEWGG
                     ESGWDTLYWYGAGIGDRWWPPGHPASCPPYGMLQVMDTSTQVIDGLPLATLHMDVLGE
                     DGLPSGMPFVIRERLGSVPRAPFIFDCGLVIDYFDASFVCYSDDQITTPGDAACGLTM
                     GVPVQAEMTAPISICPNPGTTSFTLTGLAGQRGRVRVLDAQGRTVPVGINTVGGREVY
                     AEMLCPGSYLVEVITDRGERTVLRWVKE"
     gene            1989..2180
                     /locus_tag="PL106_05290"
     CDS             1989..2180
                     /locus_tag="PL106_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32515.1"
                     /translation="MRSLHEFVDRAPHPYAVRYYAGKLSVERQKTPKGKPYILLNLPY
                     FLGTRLHAYLDWLKDEHPA"
     gene            complement(2480..2701)
                     /locus_tag="PL106_05295"
     CDS             complement(2480..2701)
                     /locus_tag="PL106_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNA32516.1"
                     /translation="MLKQPWIGQGRTLVGVFEFKTHCRLGERPDYRATKEIMVKFYEY
                     LAEGMGKADALAEAKRWYRKENPNAPPSE"
BASE COUNT          529 a          890 c          897 g          628 t
ORIGIN      
        1 cgccccggcg ccgatgaaag cgaagcggcc gatgtcgtgc ccgcaaacga tggtagcgtt
       61 ggccccgatg gatgcccctt ttttcacgag cgtgcgaagg tactcccccc tgcggttcac
      121 ggcgctgcgg gggttgatca cattggtgaa caccatgctg ggccccaaga agacgtcgtc
      181 ctcgcactcc acgcccgtgt agatgctcac gttgttctgc accttcacgt tgcggcccag
      241 cttcacctca gggctgatca ccacgttctg gccgatgttg cagttctccc cgatgacgca
      301 cccgggcatg atgtggctga agtgccagat gcgggtgcct ttgccgatcg tgcagccctc
      361 gtcgatgacg gcggtggggt gggcggtgta gtccaaggac atgctgaagg tatgcggccg
      421 gatcacgtca aacgccgttg gtgttttcgt gatgggccgc gaatgtaccg aagtgagcgt
      481 gtatcttggc aggcagcgcc aaggccttgg cgcgtgtctt tccccgaaca ccatcggcca
      541 tgcggaaaac acttctcctc ggttggttgc tcgttgcgtt ggcggcaaat gcccaactct
      601 ggtgcgcgcc cggtgtggtt tggacctatc gcttccagga ctggggaggc ggctcctctg
      661 tggactacag gaccgagtac cggtacgtgg gcgacaccct ctacaacggc atgctggccc
      721 accgggtgga ccggacatcg gcgggtgtcg cgaacggcca gccggtcggt ggcggtacct
      781 cgccgaccta cgaagccgaa gcggacgatg tggtgtgggt gtggaccacc ggtgaatggg
      841 gtggagagtc cgggtgggac accttgtact ggtacggtgc cggtatcggc gatcgctggt
      901 ggccaccggg gcatccggca tcatgcccgc cgtatggtat gttacaagtg atggacacca
      961 gcacccaggt gatcgacggc cttccgttgg ccacgttgca catggatgtg cttggcgagg
     1021 atggactccc gtcgggaatg cccttcgtga tacgggaacg cctcggctcc gtaccgcgcg
     1081 ctcccttcat tttcgattgc ggcctggtca tcgattattt cgatgcctcc ttcgtatgct
     1141 acagcgacga ccagatcacc actcccggtg atgcggcttg tggactgacc atgggtgttc
     1201 cggtacaagc ggaaatgacg gcgccgatca gcatctgccc gaacccgggc accacaagct
     1261 tcacactcac cggccttgcg gggcagcggg gccgtgtgcg tgtgctggac gcgcaaggcc
     1321 gtaccgttcc ggtcggtatc aataccgttg gaggccgtga agtgtatgct gagatgctct
     1381 gtccgggaag ctacctggtg gaagtgatca cggaccgggg cgagcgaacg gtgctgcggt
     1441 gggtgaagga gtaggctcag ccacatcata accccggttg ccgcagcatt ggtcgcacag
     1501 aatgtgtacg agctcccatc cagttatcga tcaccgatgc ggcactctct tgcaagcgcg
     1561 attggttgaa cgcccttgcg ctgcttcagc agggcgatga ggaacacgcg ctcccgctac
     1621 tggaccgcgt gatcgccggg acttgccacc cggaaccggg cttggccaag gagctgaaag
     1681 ggaagctttg agacgggtga tccggacacg tcagattggc cggcctgaac ttgggtcgta
     1741 ttcgttacaa aatatggtcg gatttgggct ggatttgtta caagaccggc caaattttgc
     1801 cccgtatccg taacaaaggc aatcacttgc tttgacgtat tgtcctttcc gcgctcggcc
     1861 cgtggttgaa cgatccccgg tataagccgc tcattatcag aggcgcggag caggtccgaa
     1921 agatcacctt tgttcgccac ggcccccacc tgatcctagt cgaagtgaag tccgggcacc
     1981 aagggcgctt gcgctccctc cacgaattcg ttgatcgcgc accgcacccg tacgccgtgc
     2041 gctactacgc gggcaagctc agtgtggaac gccaaaagac ccccaaaggc aaaccgtaca
     2101 tcctgctcaa cctgccgtat ttcctgggca cacggctgca tgcgtatctg gactggttaa
     2161 aggatgagca cccggcgtga ggccaggtga tacaccgttt atttaagccg accgtttcaa
     2221 tttacggtca tgctgaaacg cgccgccggc tttccatacg gtggtggcat cacggtatgc
     2281 tccctgaaga ccgtgtggaa gcacttgggc atttgaccgg tccgacgact cacgcagcca
     2341 gcttcgatgg tcttccgcga cgtgcggcat aggcggctgc cacgatcacc accgccgcac
     2401 cgccccacat ccatggccgc accgggctgc gcttcttcag tcgcaccggc tcattgtaac
     2461 cgatcaaaat gaacgccgct cactcgctcg gcggggcatt ggggttttcc ttgcggtacc
     2521 atcgcttggc ctccgccaac gcatcggcct tgcccatacc ctcggcgagg tattcgtaga
     2581 acttcaccat gatctccttg gtggcgcggt agtcgggtct ttcgccaagt cggcagtgcg
     2641 tcttgaattc gaagaccccg accagtgtgc ggccctgacc gatccatggc tgcttgagca
     2701 ttccacgaag ctggtattga acgatctgag ggccatgcgc gagtcgtact gccttccagc
     2761 ggccgtgatc gtgatggttc cgatgcgcat ttacgtgcgc ctgaatgccc cctttctttg
     2821 tctccctcac catgttgtcc atgttcccca tcttgcccgc tgcgttgagc gatcaggacg
     2881 atgtccgaac ttccatttga acacggcgcc acgatgctcc cgcgagcggc acaggtgggt
     2941 cttc
//
LOCUS       DAOQOC010000259         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18037_length_2933_cov_3.319666, whole genome shotgun sequence.
ACCESSION   DAOQOC010000259 DAOQOC010000000
VERSION     DAOQOC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18037_length_2933_cov_3.319666"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PL106_05300"
     CDS             <1..1086
                     /locus_tag="PL106_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA32517.1"
                     /translation="VALSRLRSLDGLILRTRIDPSVVSSDREVVAFTQRGAAQVPLPQ
                     QLQASQREYLQLLLAGTFDFGDMLRKVEYTRKDHPETAQFEDDSMKTALQLFHETLKS
                     EEENTQKFRGQLMRLLMEDRRDELLVRIEKGGTYYGELLHAQMKALYQHIAQAAMLSR
                     TKEYTNALKEIDGMLVKKIATMAKVGYLATCILNGEEVKRQQSIENDLMNRRAALVAE
                     ASAWAEEHRPKATGRSGRVRRTRSRAEGTDTSDDGIPAPKEKRAKGATYATTYALMKS
                     GFNLDQVAAERGLSKSTIEGHCARGIAEGELDIEGLLPVEDRDRIAGWMSEHPNDSLS
                     DAQKHFAGTFSYGQLRMVQAWVKREAS"
     gene            1334..2164
                     /locus_tag="PL106_05305"
     CDS             1334..2164
                     /locus_tag="PL106_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32518.1"
                     /translation="MSPSMHTPDSEGYIPFIHNYCDRRCERCRFVRQCRVGAVEVDDV
                     GEDDDVVGDERVEDLHDRMKKLLGLSDEDMAAMEEVRKEHEDEPVDPLEEAREEAAMA
                     EWQRQQEERDRQVDAQPLSVMGSTYLDLVRAWNRPREASFMARGVQLHPRMGMGIPVV
                     QRTPEVLVLSEALQEVLWFHTMIHVKIQRALHGKLEADDEEDNALQSDWNGTAKVCID
                     GVDRSMIAWDTIIELLPEEADDALPIRELLRRMRQELDAVFPDAHAFIRPGFDAPGGG
                     "
     gene            complement(2267..>2933)
                     /locus_tag="PL106_05310"
     CDS             complement(2267..>2933)
                     /locus_tag="PL106_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32519.1"
                     /translation="EFVMGDVNHDVTTTLLRAVHRLKDNRLVPIGYSVSHPSQDTTAI
                     AGLALTDPDFNHDADGVEGNGGDIVHYHIPMDGYGGAVQVKASVWFQQVPPRWNEEML
                     EYHGARIDPFRAMYEAADNTPELVVGDSLSVTPTGIAVRATDPPIRVFPNPTYDGRVL
                     IDDRAITAIVAYSATGQRVAIATDRTAQGWRCELPPQAGSYLLRITTAEDDRIVRVVR
                     ADH"
BASE COUNT          576 a          907 c          974 g          476 t
ORIGIN      
        1 gtggcgctct cgcgcttgcg ctccctggac ggattgatcc tccgaacgcg gatcgaccca
       61 tcggtggtga gcagcgacag ggaagtggtg gccttcacgc agcgcggtgc cgcacaggtg
      121 ccgctgcccc aacaactgca ggccagccag cgcgaatacc tgcaactgct gctcgcgggc
      181 acattcgact tcggcgacat gctgcgcaag gtggagtaca cgcgcaagga ccatcccgag
      241 accgcgcaat tcgaggacga ctcgatgaaa acagcgttgc agctcttcca cgagaccttg
      301 aagagcgagg aggagaacac gcagaaattc cggggccaac tgatgcgcct gttgatggag
      361 gaccgtcgcg acgaactgct ggtgcgcatc gagaaaggcg ggacgtacta tggcgagctc
      421 ctgcacgcgc agatgaaggc gctgtaccag catatcgcac aggccgcgat gctgagccgg
      481 accaaggaat acaccaacgc gctgaaggag atcgacggca tgctggtcaa gaagatcgcc
      541 accatggcca aggtgggtta cctcgccacc tgcatcctca acggtgaaga ggtgaagcgg
      601 caacagtcca tcgagaacga cctgatgaac cggcgcgccg ccctggtggc cgaggcgagc
      661 gcatgggccg aggagcaccg gccgaaagcg accggcagat cgggccgggt ccgcaggacg
      721 agatcccgtg ctgaaggtac ggatacatcg gatgatggga tccccgcacc gaaggagaag
      781 cgggcgaagg gcgccaccta cgccaccacg tatgccctga tgaagtcggg cttcaatctg
      841 gaccaggtgg ccgccgaacg cgggctgtcg aagagcacca tcgaagggca ctgcgcgcgg
      901 ggcatcgccg agggagagct ggacatcgaa gggttgctgc ccgtggagga ccgcgatcgc
      961 atcgcgggct ggatgagcga gcaccccaac gacagcctga gcgatgcgca gaagcacttc
     1021 gccggaacgt tcagctacgg ccagctccgg atggtgcaag catgggtgaa gcgcgaggct
     1081 tcctgatggt cgcgcagtcc ttggggtgaa cacgtgtcct catgttgttc agcatccgtg
     1141 atcccgcgcg cgaccaaggt ccgtttctcc ggatatgata cgggcatagt ggcgaagatc
     1201 ggccgcttcg tacatcgaac ggaccggccg gaagcgcaca ggggcgcgga gcggctgctt
     1261 tcacccgtgc acccttgcac tcccctgcca atgaaagtac ggttccttcc caccgcctca
     1321 ataccttccg accatgtccc catccatgca cacccccgac agcgagggct acatcccctt
     1381 catccacaac tactgcgatc ggcgctgcga gcggtgccgc ttcgtgcgcc aatgccgcgt
     1441 gggtgcggtg gaagtggatg acgtgggcga ggacgacgat gtggtgggtg acgagcgcgt
     1501 ggaggacttg cacgaccgga tgaagaagct gctggggctg agcgatgagg acatggccgc
     1561 gatggaagag gtccgcaagg aacacgagga cgaaccggtg gacccgctgg aggaggcgcg
     1621 cgaagaggcc gccatggccg aatggcagcg ccagcaggag gagcgtgatc gccaggtgga
     1681 cgcgcaaccc ttgagcgtca tgggctccac ctacctcgat ctggtgcggg cctggaacag
     1741 acctcgcgag gcttcgttca tggcgcgcgg tgtgcaactg catccccgca tgggcatggg
     1801 catcccggtg gtgcaacgaa cgcccgaggt gctggtgctg agcgaagccc ttcaggaagt
     1861 gctgtggttc cataccatga tccacgtgaa gatccagcgc gcgctgcacg ggaagctgga
     1921 ggcggatgat gaggaggaca acgcgctcca gagcgactgg aacggcacgg ccaaggtgtg
     1981 catcgacggg gtggaccgat cgatgatcgc ctgggacacg atcattgaat tgctgcccga
     2041 agaagcggat gacgccctgc cgatccggga actgttgcgt cgaatgcgcc aggaactcga
     2101 tgccgtcttt cccgatgcgc acgccttcat ccggccagga ttcgacgcgc ccggtggtgg
     2161 gtgatgaaca gcaccctgtc ggcatcatcc tgcacgagat ggacccggtc cggatgggat
     2221 cccggtcgtg ttcgtcggga ccggtcgcgg cgttctgtac cagcggtcag tgatccgcac
     2281 gcaccacacg cacgatccgg tcgtcctctg cggtggtgat ccgcaacagg tacgaacccg
     2341 cttgcggcgg caattcgcag cgccaccctt gagcggtcct gtcggtcgct atcgccacac
     2401 gctgcccggt cgcactgtag gcgacaatgg ccgtgatggc ccggtcgtcg atcagcaccc
     2461 gcccgtcgta tgtgggattc gggaataccc tgatcggcgg atcggtcgcg cgcaccgcga
     2521 tcccggtggg cgttaccgag aggctgtccc ccaccaccag ttcgggagta ttgtcggccg
     2581 cctcgtacat cgctcggaag ggatcgatac gcgcgccatg gtactccagc atctcctcgt
     2641 tccaacgcgg cggcacctgc tggaaccaca cgctcgcctt cacctgcacg gcgccaccat
     2701 acccatccat cggtatgtgg tagtggacga tgtcaccacc gttcccttcc accccgtcgg
     2761 catcgtggtt gaagtccgga tcggtgaggg ccaggccggc gatggccgtg gtgtcctggc
     2821 tcgggtgcga cacgctgtac ccgatcggca ccagccggtt gtccttcagg cgatgcacgg
     2881 cgcgaaggag cgtggtggtc acatcgtggt tcacatcccc catcacgaac tcg
//
LOCUS       DAOQOC010000260         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18055_length_2931_cov_2.600834, whole genome shotgun sequence.
ACCESSION   DAOQOC010000260 DAOQOC010000000
VERSION     DAOQOC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18055_length_2931_cov_2.600834"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PL106_05315"
     CDS             complement(<1..1376)
                     /locus_tag="PL106_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HNA32520.1"
                     /translation="MKRLLTSITRQGLFLLAAAWASPAVLLGQCPDNNTQVGATITPA
                     CPGTTNVPCVQGGQYALVNVITGNQYTFATCGAGFDTQISLYDTGGGLFGYNDDACGL
                     QSSITWTASFTGPLRVLVDQYFCSNGTTCAPLAITCAPPPAPVIACGTTVYDPGGAAG
                     NYANNTNYTVTYCPDDPGEVVSLNFSMFNTEATFDVVTIFNGPNTSAPVLGTFSGTGN
                     PGTITSSAPGGCLTLRFTSDGSIPYAGWAALVNCGPPPPPPSGDCILVLTLNDSFGDG
                     WGTSNVGVSINGGPYTYYTVTGSTFQTLIGVYIGQVIVLSYNNSGAFQGENSYTLGIS
                     GGGSYFNSGSPPAAGPNTFNMTVTCDPPPAPPQDCVGSTTICNGQAFNNNSSNTGNTI
                     DLTAANRGCLSSGERQGTWYVFSPSAAGTIGFTINPVVATDYDFAVWGPYPPGSTPST
                     ICPPASPPL"
     gene            complement(1373..1819)
                     /locus_tag="PL106_05320"
     CDS             complement(1373..1819)
                     /locus_tag="PL106_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32521.1"
                     /translation="MPDAIAQKSAELQYVIRVEPAFSGSTAKYIAEALLAIDPNSEVS
                     VDVPSQRVKVRTSVPVVSQSLQAGLAGSGLQVVQVQQVLPTDPIARAETIMATFGFPI
                     YVDTGHPEQDQANYATAKSAWIDADPARYQELMRQLYGEPTPLIER"
     gene            2353..2919
                     /locus_tag="PL106_05325"
     CDS             2353..2919
                     /locus_tag="PL106_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32522.1"
                     /translation="MAWKQAEKEAEQLLGIGLGHQQVFEQVRLQHPDVKPKKIAEWLR
                     QRPSNMAKDRYRTPHQVLLALVAISAALRIWSAFGHRTIDVEHAWRLIGLVPFATLFM
                     AWALYRWNGQHFQWVGWMNILGVFGMMPVLTRLASEGLITLVEVSGIISVVIGVLALY
                     LHSKAFPKYSFHKDPMGGPGRYEFRERS"
BASE COUNT          554 a          823 c         1002 g          552 t
ORIGIN      
        1 aggggtgggg aggccggtgg gcagatggtg cttggcgttg agcccggtgg atatgggccc
       61 cacacggcga agtcataatc ggtggccacc accggattga tggtgaaccc gatggtcccg
      121 gcggcggatg gggagaaaac ataccatgtt ccttggcgtt cacccgaaga gaggcagccg
      181 cggttcgctg cggtcaggtc gatggtgttg ccggtgttgc tcgagttgtt gttgaacgcc
      241 tgcccattgc agatggtggt actgcccacg cagtcctggg ggggggcggg cggcggatcg
      301 caggtcacgg tcatgttgaa ggtgttcgga ccagcggcag gtggtgatcc cgagttgaag
      361 taggagccgc caccggaaat acccaatgtg tagctgttct caccttggaa cgcccctgag
      421 ttgttgtagc tgagcactat cacctggcct atatacacgc cgatcagggt ctggaaggtg
      481 ctgccggtca ccgtgtagta cgtgtacggt cccccattga tgctcacgcc cacattggag
      541 gtcccccagc catcgccgaa actgtcattc agggtaagga cgaggatgca atcgccggaa
      601 gggggcggcg gagggggacc acagttcacg agcgcggccc agcccgcgta cgggatcgag
      661 ccgtcggagg tgaatcgcag ggtaaggcat ccaccggggg cactggacgt gatggtgccc
      721 ggattccccg tgccgctgaa ggtgcccagg accggggcgg acgtgttcgg gccattgaag
      781 atggtgacga cgtcgaaggt ggcctcggtg ttgaacatgg agaaattcaa cgacacgacc
      841 tctccgggat catcggggca ataggtgacc gtgtagttgg tattgttcgc gtaattgcct
      901 gcggctcctc cgggatcgta cacggtggtg ccgcaggcga tcaccggggc cgggggcggg
      961 gcacacgtga tcgccagtgg tgcacaggta gtgccattgc tgcagaagta ctggtcgacc
     1021 agcacccgca aaggcccagt gaatgaagcg gtccacgtga tcgaactctg caatccgcag
     1081 gcatcatcgt tgtatccgaa gagtccgcca cccgtgtcgt acaaggagat ctgcgtgtcg
     1141 aaaccggcgc cacaagtggc aaaggtgtac tggttgccgg tgatcacgtt gaccagggcg
     1201 tactggcctc cctgcacaca cgggacattg gtggtgcccg ggcaggcggg cgtgatcgtg
     1261 gcacccacct gcgtattgtt gtcggggcat tgccccagaa ggaccgccgg gctcgcccat
     1321 gcggcagcaa gcaagaagag cccctgccgg gtgatggatg tcaggaggcg cttcatcgtt
     1381 cgatcagcgg ggtgggttct ccgtacaact ggcgcatgag ttcctggtac cgggcgggat
     1441 cggcatcgat ccatgctgat ttcgccgttg cgtagttggc ctggtcctgt tccgggtgtc
     1501 cggtatccac gtaaatgggg aagccgaacg tggccatgat cgtctccgct cgagcgatcg
     1561 ggtcggtcgg caacacctgc tgcacctgga ccacttgcag gccactaccc gccaaaccgg
     1621 cttgcagtga ttgagacacc accgggaccg aggtgcgcac cttcacccgt tgcgaaggca
     1681 catccacgga gacctcgctg ttggggtcga tggccagcag ggcttccgcg atgtatttgg
     1741 ccgtggagcc cgaaaaagca ggctccaccc ggatcacgta ttgcagttca gccgatttct
     1801 gggcgatcgc atcgggcatc acccccaagg tcagaaccgg cagaaaacct gatcttatgt
     1861 agcggacaag cgtagtggtt accttcattg agcaggttta cgtatccaaa acgctgcaaa
     1921 ttactcaggg ttgcctgtgg ttgtaaagtc cgagcaggaa cgcctggact tggaccggtc
     1981 ggggcggctg tgcaacaggc tatatagaag atccgggtag cgtgctgcct ccgccaggga
     2041 tatgccttcg cccgcagagc cgggctacgt cgtacaccgt ggcagcggca gcttgccgaa
     2101 ggccgggcct tgggggcggt agcggcgagg aggcgaccgg aggaagcccc cggagcgcag
     2161 cgcccgcagg accggacgag ggcaccacag cccttcggcc ctgctcagga taaactccca
     2221 gcccgcccgg gcggccgaaa gatgacccga ggagatcggt gtcacgatcg tagatcccct
     2281 gtggaccgtg cagttgaccg gccagcacaa gcgagtgtac gggtcgggtg atccctgcat
     2341 cttcgcctcg ccatggcgtg gaagcaggcc gagaaggagg ccgaacaact cttggggatc
     2401 ggcctcggcc accaacaggt gttcgaacag gtgcggctgc aacacccgga cgtgaagccc
     2461 aagaagatcg cggaatggtt gcggcagcgg ccttccaaca tggcgaagga tcgttaccgt
     2521 acgccgcatc aggtgctgct ggccttggtg gcgatcagtg ccgcgttgcg gatatggtcc
     2581 gcttttggcc accgcacgat cgatgtggag catgcctggc ggctgatcgg gctggtgccc
     2641 ttcgccacgc tcttcatggc ctgggcgctg taccgatgga acggccagca cttccagtgg
     2701 gtgggctgga tgaacatcct cggcgtgttc ggcatgatgc ccgttctgac caggctggca
     2761 tcggagggcc tcatcaccct ggtcgaggtg agtggtatta tttccgtggt catcggagtg
     2821 ctggcgctgt acctgcacag caaggccttt ccgaagtaca gcttccacaa ggatcccatg
     2881 ggcgggccgg ggcggtatga attccgggag cggtcgtaag gagtggaatt g
//
LOCUS       DAOQOC010000261         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18087_length_2928_cov_3.586843, whole genome shotgun sequence.
ACCESSION   DAOQOC010000261 DAOQOC010000000
VERSION     DAOQOC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18087_length_2928_cov_3.586843"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PL106_05330"
     CDS             complement(<1..680)
                     /locus_tag="PL106_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNA32523.1"
                     /translation="MSVDLIIRSHAGEVAIAVLRDKLLMELHRERTEKGFAVGDIYLA
                     KVRKVAPGLNAAFVDVGHEKDAFLHYYDLGPQYRNSFKFTKAAVQGNQKGSLLEDWKL
                     DPDISKEGKLGDVVSASQSVLVQIAKEPISTKGPRLTAEVTLAGRYMVLVPFINKVSI
                     SSRITDEVERKRLKELMHAIRPKNFGMIVRTNAEGKKSEDLEADLKNLLQRWDVMFRN
                     LRNALPPKR"
     gene            complement(887..1453)
                     /locus_tag="PL106_05335"
     CDS             complement(887..1453)
                     /locus_tag="PL106_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32524.1"
                     /translation="MALQRKHILMIAGAGVCLLLLSLAPRAPKKAVDAEASARVSEDP
                     NSGSTPKAEDAHDAALSKDPAVNSILHELNDAMSAGQPPMAAITKLRDLADEHPDNVE
                     AQFHMGMLSWQSGQYDKSMERLRKVIALDPKGYPDAWAYLGQAYGTLDSLDKAIAAIE
                     TYKTLVTDTALLHGADRFLTDLRTKKNK"
     gene            complement(1487..1780)
                     /locus_tag="PL106_05340"
     CDS             complement(1487..1780)
                     /locus_tag="PL106_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNA32525.1"
                     /translation="MTKAELVANISKRTGIERGIVLATVEAFMDEVQNQLEAGEGVHL
                     RGFGSFLVKHRAQKTGRLLAQNTTIIIPAHNVPTFKPADVFVDKIKSASQKSA"
     gene            1879..2889
                     /gene="mutY"
                     /locus_tag="PL106_05345"
     CDS             1879..2889
                     /gene="mutY"
                     /locus_tag="PL106_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008989937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNA32526.1"
                     /translation="MLPWYREHGRTLPWRGKPDPYRVWLSEVILQQTRVDQGLAYWQR
                     FVDRFPGVQDLAAATEGEVLKLWQGLGYYSRARNLLVAARQVVRDFRGTFPGTHAELI
                     TLKGVGDYTASAIASICFGQPDAVVDGNVYRVLARVFGIEIPVDSTEGRKRFKALAQE
                     LLDHDHPGDHNQAVMELGATVCTPKNVACLLCPVQGRCIAFASGRIDALPVKKGVAKT
                     RDRYFNYLHIRTSNGLYLRQRTGKDIWQGLWEPPLIESGAPLTAKKLQERLGPGWQVR
                     SRGEEAKHVLSHQVIRAVFWDVTPPKCEKLDPGWKAMDAAAVRAVAVPRLIEQWLKRA
                     GI"
BASE COUNT          494 a          875 c          937 g          622 t
ORIGIN      
        1 cgcttgggcg gcagggcgtt gcgcaggttg cggaacatca cgtcccaccg ctgcaacagg
       61 ttcttcaggt cggcctcgag gtcctcgctc ttcttgccct ccgcgttcgt gcgcacgatc
      121 atgccgaagt tcttcggccg gatcgcatgc atgagctcct tcaggcgctt gcgctccacc
      181 tcatcggtga tgcgcgagga gatgctcacc ttgttgatga agggcaccag caccatgtaa
      241 cggcccgcca gcgtcacctc tgcggtgagg cgcggaccct tggtgctgat gggctccttg
      301 gcgatctgca ccagcacgct ctggctggcg ctcaccacat cacccagctt gccctccttg
      361 ctgatgtcgg ggtccaactt ccagtcctcg agcagcgaac ccttctggtt gccctgcacg
      421 gctgccttgg tgaacttgaa gctgttgcgg tattgcggtc ccaggtcgta atagtggaga
      481 aaggcgtcct tctcgtgccc tacgtccacg aacgcggcgt tgaggcccgg ggccaccttg
      541 cgcaccttgg ccaggtagat gtcgcccacg gcgaagcctt tttcggtgcg ttcgcgatgc
      601 agctccatga ggagcttgtc gcgcaggacc gcgatggcca cttcgcccgc gtgggaacgg
      661 atgatgagat ctacactcaa tggaagccgt gttgtgcgta cccgatcgga tacacgacag
      721 ccgttcggcc gccgtcccaa ggcagtggga tgcggcgtgc cggatcagcc atcacagcag
      781 gtccggtact accggatctt cttcttgtga cggttcttcc gcaggcgctt cttgcgcttg
      841 tgggtggcca tcttgtggcg cttccgcttc ttaccgcagg gcatggttac ttgttctttt
      901 tcgttctcag atcggtgagg aagcggtccg ccccgtggag cagtgccgta tccgtcacca
      961 gtgttttgta ggtttcgatg gccgcaatgg ccttgtccag gctgtcgagc gtgccgtagg
     1021 cctgtccgag gtaggcccaa gcatccgggt agcctttggg atccagggcg atcaccttcc
     1081 tgaggcgctc catggacttg tcgtactggc cgctctgcca actgagcata cccatgtgga
     1141 actgggcctc cacattgtcg ggatgctcgt cggccaggtc gcgcaacttg gtgatggcgg
     1201 ccatgggcgg ttgtcccgcg ctcatcgcat cgttcaattc gtgcaggatg gaattcaccg
     1261 cgggatcctt gctgagcgcc gcatcgtgcg cgtcctccgc tttcggggtt gaaccgctgt
     1321 tggggtcctc gctgacacgg gcgcttgctt cagcatccac cgccttcttg ggcgcgcggg
     1381 gagccaacga caacagcaac aggcatacgc cggccccggc gatcatcagg atgtgtttgc
     1441 gctgcagggc catgggtgct ggttcctatc gcggtcaatg ttcggctcag gccgacttct
     1501 gcgatgcgct tttgatcttg tccacgaaca catcggccgg cttgaaggtg ggcacgttgt
     1561 gcgcggggat gatgatggtg gtgttctggg ccagcaggcg accggtcttc tgggcgcggt
     1621 gcttcaccag gaaactcccg aagccccgca ggtgaacgcc ttcccctgcc tccaactggt
     1681 tctgcacctc gtccatgaag gcttccactg tggccagcac gataccgcgc tcgatccctg
     1741 tccgtttgct gatgttcgcg acaagttccg ccttggtcat tacgggtcgt tctttatttg
     1801 ggggcgcgaa tatagccttc taatcggttg agaaacacat ccttgtcaac aagaaacaca
     1861 cgctggttca gtcaggcgtt gcttccgtgg taccgcgagc atgggcgaac cctgccttgg
     1921 cgaggcaaac cggatccgta ccgggtatgg ctgagcgagg tgatcctgca acaaacccgc
     1981 gtggatcaag gacttgccta ctggcagcgg ttcgtggacc ggtttccggg cgtgcaggac
     2041 ctggctgcgg ccaccgaggg tgaggtgctg aagttgtggc agggcctggg ctattacagc
     2101 cgggcccgga acctgctggt cgcggcccgg caagtggtcc gcgatttccg gggaaccttt
     2161 ccgggcaccc atgccgaact gatcacattg aaaggtgtgg gggattacac ggcctcggcc
     2221 atcgcctcta tttgtttcgg ccagccggat gcggtggtgg acggcaatgt gtatcgcgta
     2281 ctcgcccgtg tgttcggtat cgagatcccc gtcgattcca cagaaggcag gaagcggttc
     2341 aaggcactgg cccaggagct gctggaccat gatcatcccg gtgaccacaa ccaggcggtg
     2401 atggaactcg gggctacggt gtgtaccccg aagaacgttg cgtgcctgct ctgccccgtg
     2461 caaggcaggt gcatcgcgtt cgccagcggg cgcatcgatg cgttgcccgt gaagaaaggg
     2521 gtagcgaaaa cccgggaccg ctacttcaac tacctgcaca tccgcacgag caatggcctc
     2581 tacctccgcc aacgcaccgg taaggacatc tggcaggggc tgtgggaacc gcccttgatc
     2641 gaaagcggtg cgccgctaac ggcaaagaag ctgcaggaac ggctcgggcc gggctggcag
     2701 gtacgttcga ggggtgaaga ggcgaaacat gtgctcagtc atcaagtgat cagggcggtg
     2761 ttctgggatg tgaccccccc gaagtgcgag aaacttgacc ccggctggaa ggccatggac
     2821 gcggcagcgg tccgtgcggt ggcggtgccc cgtctgatcg agcagtggct caagcgggca
     2881 gggatctgac ccgacgaccg gcgcattccg aacaaaggtg gatgatcg
//
LOCUS       DAOQOC010000262         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18100_length_2927_cov_3.548747, whole genome shotgun sequence.
ACCESSION   DAOQOC010000262 DAOQOC010000000
VERSION     DAOQOC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18100_length_2927_cov_3.548747"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PL106_05350"
     CDS             complement(<1..740)
                     /locus_tag="PL106_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187131.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNA32527.1"
                     /translation="MSHAVAAPYVPKHKIRVVTAASLFDGHDAAINIMRRIIQATGAE
                     VIHLGHDRSVEDVVHTAIQEDAHAIAMTSYQGGHMEYFKYMYDLLKEKGAGHIKIFGG
                     GGGTILPEEIKELEAYGIARLYHPDDGRAMGLQGMINDMLEKADYDLSDQVNGEAKQL
                     TTHNWTAIAKLISTAENHPEVFAKVADEIHAKAAKNKAPVLGITGTGGAGKSSMVDEL
                     IRRFILDQPTKTIGVISVDPSKRKTGGAL"
     gene            1212..1871
                     /locus_tag="PL106_05355"
     CDS             1212..1871
                     /locus_tag="PL106_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32528.1"
                     /translation="MEIGLDPKVAALPLAKRRSILLQCINEGKRALEAASPGITKQME
                     QFADSPFRFTASLTADQIRAIWHLRELRHLSDRDDNPWMDVQPDKNGLFPFIVLVHQL
                     HQQEGRKRIEVQELTLLIQSKDNRTAKADALRECRTQSAHVMNDDFKVTKRWWVAKRA
                     WLNTLREEKHSSIGQALLIDAINTWTEPAKEIWRPSKPMKDITIHSTRKRPKTWEWML
                     N"
     gene            1763..2680
                     /locus_tag="PL106_05360"
     CDS             1763..2680
                     /locus_tag="PL106_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32529.1"
                     /translation="MDGACQGNMASIQANEGHHHTQHKKKTEDMGVDVELNQLEKDLL
                     RRYQEIEQAYRAENKQAVAPWWAYPVPPPIPFVGSAFGESGRPKVLVYASAENLSNPG
                     QVVGLRNAAAMCRSRRAWLDGGKQHDVHIEPINNGGLLKVARHIIEELGYRDQFSCTP
                     RSFLEEIAVANPGKFCIDPSKFEKKVNLDYARDWKKFKEQTAYIRADLKRLSPRVLIL
                     PATIHRSLCRTDLKPELDLVPKVVLINQVQQRAIFRKHGNRVPKDNAATLPSGYGDWK
                     APPYANSYIRWMMENRNEFNRKENGQVTL"
BASE COUNT          618 a          843 c          857 g          609 t
ORIGIN      
        1 agtgcgccgc cggtcttgcg cttgctgggg tccacgctga tcacgccgat ggtcttcgtg
       61 ggctggtcca ggatgaagcg gcggatcagc tcatccacca tgctgctctt gcccgcgccg
      121 cccgtgccgg tgatgccgag gaccggtgcc ttgttcttcg cggctttcgc gtggatctcg
      181 tcggccacct tggcgaacac ctccgggtgg ttctccgccg tgctgatcag cttggcgatg
      241 gccgtccagt tgtgcgtggt cagctgcttg gcctcgccgt tcacctggtc gctcaggtcg
      301 taatcggcct tctccagcat gtcgttgatc atgccctgca ggcccatggc gcggccatcg
      361 tccgggtggt acagccgcgc gatgccgtag gcctccagct ccttgatctc ctcgggaagg
      421 atcgttccgc cgccgccgcc gaagatcttg atgtgccccg cgcccttctc cttcagcagg
      481 tcgtacatgt acttgaaata ctccatgtgc ccaccctggt agctggtcat ggcgatggca
      541 tgggcgtcct cctggatcgc ggtgtgtacc acgtcctcca cgctgcggtc gtgccccagg
      601 tggatcacct cggcgccggt ggcctggatg atgcgccgca tgatgtttat cgccgcatca
      661 tgcccatcga agagcgaggc ggcggtaacg acgcggatct tgtgcttggg gacgtagggg
      721 gcggcaacgg cgtgtgacat ggtgcgaaaa taccggagga cggggaatct gctctggccg
      781 cccgggcggg ctgtccgctg tagccggact tgtcacgggc cacatcaccg gcgctgcgcg
      841 ggcttcctgc ggtcgcctgc tcgctgcgcg gtgctgttgg cccggtcctg cgccgggctg
      901 ccgcttccat cccgggcgga gatcacgttc atggcaccgt tgatacattg ccatcacaaa
      961 caaacaacca tggcacaagc agtagcactt tcaggggccg tcattatctc acaggaggga
     1021 tcccgattgg tgtacaaggg taaatgcgag aaatgtggtt cgatctcgaa ctccgaggtt
     1081 agcacccatt ccgtttcaaa aggccagaca tacggctcca cgtatcactg ccacaaatgc
     1141 aagaatgccc agcgcattca gatcaaggga ttctgatggc caggtcaagg acatatcgtg
     1201 cttggctcga attggagatc gggctggatc cgaaggtggc tgcgttgccg cttgccaagc
     1261 gccgaagcat cctccttcag tgcatcaatg agggcaagcg ggcgctggag gcggcatctc
     1321 ctgggatcac caagcaaatg gagcagtttg ccgactcgcc gttccgtttc acagcgtctc
     1381 ttaccgccga tcagatccgc gcgatctggc accttcggga actgcgccat ctgtccgatc
     1441 gggatgacaa cccgtggatg gacgttcagc ctgataagaa tgggctgttt cccttcatcg
     1501 tccttgtgca tcagcttcac cagcaagagg ggcgcaagcg catcgaagtg caggaactca
     1561 ccctactgat tcaatccaag gacaaccgga cggccaaggc cgatgcactg cgcgaatgcc
     1621 gtacacaatc cgcccatgtc atgaacgacg acttcaaggt caccaaacgc tggtgggtgg
     1681 ccaagcgggc atggctcaac acccttaggg aggagaagca ctcctcgatc ggccaagctt
     1741 tgctcatcga tgcgatcaac acgtggacgg agcctgccaa ggaaatatgg cgtccatcca
     1801 agccaatgaa ggacatcacc atacacagca caagaaaaag accgaagaca tgggagtgga
     1861 tgttgaactg aatcaattgg agaaggacct tcttcgtcga tatcaagaaa tcgaacaggc
     1921 gtatcgcgct gagaacaagc aggccgtcgc accttggtgg gcatatccgg taccaccgcc
     1981 gattcctttc gttgggtctg cattcggcga gagtggaagg cctaaggttc ttgtctatgc
     2041 gagcgcagag aatctctcga atccaggaca agtcgtgggg ttgagaaatg cagcggcgat
     2101 gtgccgctct agacgggcat ggctggatgg cggaaagcag cacgatgttc atatcgaacc
     2161 catcaataac ggtggacttc tgaaggtcgc tcggcacatc atcgaagaac ttgggtaccg
     2221 ggatcaattc agttgcacac ccaggtcgtt tcttgaagag atcgctgttg cgaatccggg
     2281 caaattctgt atcgacccat ccaagttcga gaagaaggtg aatctcgact acgccaggga
     2341 ttggaagaaa ttcaaagaac agacagcgta cataagggca gacctgaagc gactttctcc
     2401 tcgggtgctg atcctgccgg caacgatcca tcgctcgttg tgccggactg atttgaagcc
     2461 cgaacttgac ctcgttccga aggtcgttct gatcaaccag gttcagcaaa gagcgatttt
     2521 ccggaagcat ggcaatagag ttccaaagga caacgctgca acgttgccca gcggttacgg
     2581 agattggaag gcacctccgt atgccaactc gtacatccgc tggatgatgg agaataggaa
     2641 cgaattcaac cggaaggaaa atgggcaagt aactctatag cgcagtatgt actacgtcct
     2701 ccatgccgcg atcgagcccc aggtggatca cctcggcggc ggtgctctgg atgatcagct
     2761 tcaagcgaac cgcgttacgt tcgcacgtct tggcattgat gcagtgatgc gcaccatatt
     2821 tctgttcgct gctctggtcc cgctcagcgt tctggcctgc accaccttca aggtcaccca
     2881 ggacggccac accctcatcg gcggcaacga ggatgcatgg agcatca
//
LOCUS       DAOQOC010000263         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18111_length_2926_cov_4.177987, whole genome shotgun sequence.
ACCESSION   DAOQOC010000263 DAOQOC010000000
VERSION     DAOQOC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18111_length_2926_cov_4.177987"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="PL106_05365"
     CDS             <1..2107
                     /locus_tag="PL106_05365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNA32530.1"
                     /translation="NFTGIERIQTGVGNDLIDLSSLRPEGATVSNAARTSTGAVSFME
                     RFSNTTREVAQKGRRGALMLTMDIAHPDVERFITIKQDLTKVTGANVSVRVSDEFLRA
                     VSEGGTYTHRWPIDAREPLVTKEVNARDLWNTLVSCAHNSAEPGLIFWDRQHTYSTSS
                     VYPAFKNESTNPCSEIAMQGGDSCRLIAINLYSFVQDAFTPKAWFDHDRFAAVTYESQ
                     RLMDDLVDLELEAVDRILAKVDSDPEPEEVKRVERETWELLRDTGRKGRRTGLGFTGM
                     ADALAALGLKYDSDAAIEATESIMRTKCRAEFDSSIDMAIERGAFEGFDPAIERTSGF
                     VSMLADELPEVHDRMMRHGRRNVSISTVAPTGTLSLLTRTSSGIEPVFMLGYTRRRKI
                     LAGDGKAVVSFTDNMGDQWEEFTVHHPRLLDWMKVTGKSDAAQSPYHGATANDIDWQR
                     RIRMQAAVQKYVTHSISSTINLPNTATVEQVGGIYLEAWQHGLKGITVYRDGSRSGVL
                     VSTDAKKEERPHHITRPLRLEADVLRFNNGTDPWLAVVGLMDGRPYEIFTGRANGNFE
                     LPKWVTKGWVVKRADPERGKNIYDLEYADDEDYRVTVQGLSRTFDKEFWNYAILISGM
                     LRQGMPLPQVVDVVANLNLYDATLGTWKNGVVRALQRYIADGTVASGRKCADCGDVDG
                     LHYEEGCLKCKSCGSSKCG"
     gene            complement(2530..>2926)
                     /locus_tag="PL106_05370"
     CDS             complement(2530..>2926)
                     /locus_tag="PL106_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA32531.1"
                     /translation="VAGPAAETQKKEQLDTATQNYGGARRIALPEERGFEIVDLDAII
                     HCQSDSNYTKVFLRDKRMLLVCRTLKDIGALLDEPRFFRVHDSHIVNMDHTTRYIRGD
                     GGELILDNGQNVPVSRSKKEELMGRLNKA"
BASE COUNT          578 a          890 c          945 g          513 t
ORIGIN      
        1 gaatttcacc gggatcgagc ggattcagac cggtgtcggc aacgatctga tcgacctcag
       61 cagcctgcgc cccgaaggtg ccacggtgag caatgctgcg cgcaccagca ccggggcggt
      121 gagcttcatg gagcgtttca gcaacaccac gcgcgaagtg gcgcagaagg gccgtcgcgg
      181 cgcgctgatg ctcaccatgg acatagccca tcccgacgtg gagcggttca tcaccatcaa
      241 acaggatctt acaaaggtca ccggtgccaa tgtgagcgtg cgcgtgagcg acgagttcct
      301 gcgggccgtg tccgaaggtg gcacgtacac gcaccgctgg ccgatcgatg cccgggagcc
      361 gctggtcacc aaggaggtga acgcccgcga tctctggaac acgctggtga gctgtgcgca
      421 caacagcgcc gagccgggcc tcattttctg ggaccggcag cacacgtaca gcaccagcag
      481 cgtatacccg gcattcaaga acgagagcac caacccctgc agcgagatcg ccatgcaggg
      541 cggcgacagt tgtcgtttga tcgccatcaa cctgtacagt ttcgtgcagg atgccttcac
      601 gccgaaagcc tggttcgatc atgaccggtt cgcagcggtc acctacgagt cgcaacgcct
      661 catggacgac ctggtggatc tcgaactcga ggcggtggac cggatcctcg ccaaggtgga
      721 cagcgacccc gaacccgagg aggtgaagcg tgtggagcgc gagacctggg aactcctgcg
      781 cgataccggt cgcaagggac gccgcaccgg attgggcttc accggcatgg ccgatgcgct
      841 ggccgcactg ggcctgaagt acgacagcga cgcggccatc gaggccacgg agtcgatcat
      901 gcggaccaag tgccgtgccg aattcgacag cagcatcgac atggccatcg agcgcggcgc
      961 tttcgagggc ttcgatccgg cgatcgaacg cacgagcgga ttcgtatcga tgctggccga
     1021 cgaactgccc gaggtgcatg accggatgat gcgtcatggc cgccgcaacg tgagcatcag
     1081 caccgttgcg cccaccggta cgctcagtct gctcacccgc accagcagtg ggatcgaacc
     1141 cgtgttcatg ctgggctaca cgcggcggcg caagatcctc gcgggggacg ggaaggccgt
     1201 ggtgagcttc accgataaca tgggtgatca atgggaggaa ttcaccgtcc accacccgcg
     1261 tctgctcgac tggatgaaag tcaccggcaa gagcgatgcc gcacagagcc cgtaccacgg
     1321 tgccaccgcg aacgacatcg actggcagcg tcggatccgc atgcaggccg ccgtgcagaa
     1381 gtacgtcacg cactccatca gcagcaccat caacctgccg aacaccgcca cggtggagca
     1441 ggtgggcggc atctacctgg aggcctggca gcacgggctg aaaggcatca ccgtttatcg
     1501 cgatggcagc cggagcggcg tactggtgag caccgatgcg aagaaggagg agcgcccgca
     1561 ccacatcaca cgtcccttgc gcctggaagc ggatgtactg cgtttcaaca atggaacgga
     1621 tccctggctc gccgttgtgg gcctgatgga cggtcggccg tacgagatct tcaccggccg
     1681 cgcgaacggg aactttgagc tgcccaaatg ggtcaccaag ggatgggtgg tgaagcgtgc
     1741 cgatcctgag cgtggaaaaa atatttatga cctggagtat gccgacgacg aggactaccg
     1801 cgtcacggtg caagggctca gccgcacgtt cgacaaggag ttctggaact acgccatcct
     1861 gatcagcggc atgctgcgcc agggcatgcc tctgccccag gtggtggacg tggtggccaa
     1921 cctcaacctg tacgacgcca cactgggcac ctggaagaat ggcgtggtgc gcgccctgca
     1981 acgctacatc gccgatggca ccgttgccag tggccgcaag tgcgccgatt gcggcgatgt
     2041 ggatgggctg cactacgagg agggctgcct caagtgcaag agttgcggca gctccaagtg
     2101 cgggtgatac cattcgcgca tccaaggtcg gacaggagat acgccacgat cttttcggag
     2161 gacgattcac ggaggtggtc gcgtagcgcg tctccgacgc gccaagccgg ctccgtgctt
     2221 cgcgacggcg cagttggcga cggggcggcc tacgggacca cctccggcga gaagtcatcc
     2281 ggaaagaggg ctacaggacc tgtaccgcaa gaagcggcat gggtcggtat tgaatgtgcg
     2341 atcggtataa tattgctggg agccatgggc atcacgttac gcgatggggc gctcgcgcga
     2401 tgctgcattc aacccgcaaa tgcaatcgtg gactggtcgg ttgatcgctg ttgatcctgg
     2461 ttctttgaag tgggttcgaa tgtgtgcgtg gctccggcat ccagcgcacc ccgatcgtgc
     2521 atatccccat cacgccttgt tcaatcgccc catcagctcc tccttcttgc tccgactcac
     2581 cggcacgttc tgtccgttgt ccaggatcag ctcgccacca tcgccgcgga tgtagcgggt
     2641 ggtgtggtcc atgttcacga tgtgcgagtc atgcacgcgg aagaagcgcg gctcatcgag
     2701 cagggcgccg atgtccttca gcgtgcggca taccaacagc atgcgcttgt cccgcaggaa
     2761 gaccttggtg tagttgctgt cgctctggca atggatgatg gcatcgaggt ccacgatctc
     2821 gaagccgcgt tcttcgggca gggcgatgcg tcgggccccg ccgtagttct gcgtggcggt
     2881 atcgagttgc tccttcttct gcgtttcggc cgcgggcccc gccact
//
LOCUS       DAOQOC010000264         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18123_length_2925_cov_3.467247, whole genome shotgun sequence.
ACCESSION   DAOQOC010000264 DAOQOC010000000
VERSION     DAOQOC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18123_length_2925_cov_3.467247"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..697)
                     /locus_tag="PL106_05375"
     CDS             complement(152..697)
                     /locus_tag="PL106_05375"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HNA32532.1"
                     /translation="MTYVVRLPEVLRWSDFVKTEGKQRDPHDKQEIVALTRFRFTFPN
                     EGFRPVTDAGKRKLVFTDKFKLTIRPVAKVWKGLDVTDSTSDAILAHEQIHYLFGFVT
                     ARALMNELLSLQVANDRLLKQHMGSALDLHLITRAKALNENYDDDTEHGHNATLQRKW
                     QAAMKDCLADPDATYLMGQPL"
     gene            complement(795..1184)
                     /gene="tssD"
                     /locus_tag="PL106_05380"
     CDS             complement(795..1184)
                     /gene="tssD"
                     /locus_tag="PL106_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HNA32533.1"
                     /translation="MAFRADLEMNGKKYRLLHCNYALNRDVDHTGRPSSEVKGGTIQF
                     EIESTEDTSIWDLMIAQFKSTDGTITFKKRDEDAKMKELKFETGYVVALSENFDSTGG
                     NPLSMSFTISAHKLSLGNEKLENEWPI"
     gene            1343..>2925
                     /locus_tag="PL106_05385"
     CDS             1343..>2925
                     /locus_tag="PL106_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNA32534.1"
                     /translation="MRYLLWFFLTSASLAVEAQRMPFTYQDMLMLDRISGLSVDAAGK
                     RILFNVRATDMEKNKGVSSLWLKDLGAPTSRERRLAVSDSGAYSAQWDPAGKTIYFLS
                     ARGGSGSRQLWKTDPEGRTAQQVTRLALGIDAFRITPDGLGAVVALAVFPSATEDEIA
                     LTVRKQGERSMGSGTVYDRVFVRHWDTWADGTRSHLFHVDFGSGRVTALTQGFDGDIP
                     SKPFGDEGDYVIAPDGRTVYFSARVAGKGEPWSTNFDIYSVPLTGGAATNLTVTNAAW
                     DASPRISPDGKTLAYTAMERPGFEADRFEVRLRDLVTGTTRALAATWDRSVSTLQWAP
                     DGKGLYVTADDMGRHRLFRIDVKSGRVTTLSKDGHIDAFVKTAQGFVFLKSGLNGPSQ
                     LYASTPKSSLIDEGANKLTAVNAVLDQKEFGVYEQFSFPGWNNETVHGFVIKPVGYQE
                     GKKYPVAFLIHGGPQGSFGDGWSFRWCPQTYAGAGYAVVMIDFHGSTGYGQAFTDAIS
                     THWGDRPLEDLQKGWVHAL"
BASE COUNT          552 a          821 c          950 g          602 t
ORIGIN      
        1 attgctcgtg aaggcccatc ggtgatccgc gcagcgcgcc gggcaaaggt actgggggtc
       61 gggggctgtc gcatgaggtg ttcacagccc catccgacgg ctgggaaccg gatggacgat
      121 accgtaggaa ggggatctgc cgtcggggcg atcagagagg ctgacccatc aggtaagtgg
      181 cgtcgggatc cgccaggcag tccttcatgg cggcttgcca cttccgctgc aaggtcgcat
      241 tgtgcccgtg ttcggtgtcg tcgtcatagt tctcgttgag cgcctttgct cgcgtgatga
      301 ggtgcagatc gagcgccgat cccatgtgtt gcttgagcag tcggtcattc gctacctgga
      361 ggctgagcag ctcgttcatc aaggcgcggg ccgtcacgaa tccgaacagg tagtggatct
      421 gttcatgggc caggatggcg tcgctggtgc tgtccgtgac gtcaaggcct ttccacactt
      481 tggccactgg ccggatggtc agtttgaatt tgtcggtgaa gacaagcttg cgcttacccg
      541 cgtccgtaac agggcggaac ccctcgttgg gaaatgtgaa tcggaaccgg gtcagggcaa
      601 cgatctcctg cttgtcgtgg gggtcgcgtt gcttcccttc ggtcttgacg aagtcggacc
      661 accgtaaaac ctcggggagc cttaccacat acgtcatgta gcgtggggat attcagacat
      721 gcaacataaa taacggagtt gggaaaaata gggcgcggcc atgtcgccac ggccacgccc
      781 cttggatcat gtgttcagat gggccactcg ttctcgagtt tctcgttccc caggctgagc
      841 ttgtgcgcac tgatggtgaa gctcatgctc agcgggttcc cgcccgtgct gtcgaagttc
      901 tcgctcaagg ccaccacgta gcccgtctcg aacttcaact ccttcatctt ggcgtcctcg
      961 tcgcgcttct tgaaggtgat ggtgccgtcg gtgctcttga actgggcgat catcaggtcc
     1021 cagatggagg tgtcctcggt gctctcgatc tcgaactgga tcgtgccgcc tttcacttcg
     1081 ctgctggggc ggccagtgtg gtccacatcg cggttgaggg cgtagttaca atgcagcaat
     1141 cggtattttt taccgttcat ttcgaggtca gcgcggaatg ccatggttga taagtgttcg
     1201 tggttcgttg tgcgttgttg acgaggcgaa aatgggatgc tcattcagca tgcgtatgcg
     1261 cgatcgagca actggtggag cctcgaaagc gagtggcggc ttcgggcaag gcatttagat
     1321 ttcggcccac acaaccgacc ccatgcgtta ccttctttgg ttctttctga cttcggcgtc
     1381 tctggccgtg gaggcccaac ggatgccatt cacctaccag gacatgctga tgctcgaccg
     1441 catcagtggc ctgtccgtgg atgctgcggg taagcggatc ctgttcaacg tacgtgcgac
     1501 ggacatggaa aagaacaagg gagtatcgtc gctctggttg aaagacctgg gagcgccgac
     1561 ctcccgggag cgccggctgg cagtgagtga cagtggtgcg tacagcgcac aatgggaccc
     1621 ggccggcaag accatctact ttctctcggc acgtggcggc agcggcagtc gccagctttg
     1681 gaagacggac ccggaaggac gcaccgcgca gcaggtaacc cggttggcgc tgggcattga
     1741 tgccttccgc attactcccg acggactggg tgcggtggtc gccctggccg tgttcccatc
     1801 agcaacggaa gatgagatcg ccctcacggt gcgcaagcag ggcgaacgct ccatgggcag
     1861 tggaacggtg tacgaccggg tgttcgtacg gcactgggac acatgggcag atggtacgcg
     1921 gagccacttg ttccatgtgg acttcggcag cgggcgggtg acggcgctca cccagggctt
     1981 cgatggcgac atcccttcaa aacccttcgg cgatgagggt gactacgtga tcgccccgga
     2041 cggtcgtacg gtgtacttca gcgcgcgagt tgctggtaaa ggcgaaccat ggagcaccaa
     2101 cttcgacatc tacagcgtgc cgctcaccgg cggtgccgca accaacctga ccgttaccaa
     2161 tgcggcctgg gacgcgagcc cgcgcatatc gccggacggg aagacactgg cttacacggc
     2221 catggaacgg cccggtttcg aggcggaccg ctttgaagtg cggttgcgcg acctggtcac
     2281 cggcactacg cgcgcacttg cggccacgtg ggaccgtagt gtgagcacgt tgcaatgggc
     2341 tccggacggc aagggtctgt atgtgaccgc ggacgacatg ggccggcacc ggctcttccg
     2401 gatcgatgtg aagagcggcc gggtgaccac cctctcgaaa gacggccata tcgacgcctt
     2461 cgtgaagacg gcccaaggtt ttgttttcct gaagagtggg ctaaacggcc catcgcagct
     2521 gtatgcatca acccccaagt cctcgctcat tgatgaaggc gcgaacaagc tgaccgccgt
     2581 gaacgcagta ctggaccaga aggagttcgg ggtgtatgag cagttcagct tcccggggtg
     2641 gaacaacgag accgtgcatg gcttcgtgat caaaccggtc ggctatcagg aaggaaaaaa
     2701 ataccccgtg gcctttctca tccacggtgg gccacaaggc agcttcgggg acgggtggag
     2761 cttccgctgg tgcccgcaga cgtacgcggg tgcgggctac gcggtggtga tgatcgattt
     2821 ccatgggagc acgggctacg ggcaggcctt caccgacgcc atcagcaccc actggggcga
     2881 taggccgctg gaggacctgc aaaaaggctg ggtgcacgca ctgag
//
LOCUS       DAOQOC010000265         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18132_length_2924_cov_3.681771, whole genome shotgun sequence.
ACCESSION   DAOQOC010000265 DAOQOC010000000
VERSION     DAOQOC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18132_length_2924_cov_3.681771"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>2924)
                     /locus_tag="PL106_05390"
     CDS             complement(184..>2924)
                     /locus_tag="PL106_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32535.1"
                     /translation="GGDTLFWPHEIHRPACPYCVCDPARTISWNVDEVWSTGASDMDT
                     IHAWSTGWYWRTQTASNGCYTSTDSIHLTVLPAPIAWASLGGAPAFPGPFNVFSICEV
                     PTTLETNNLQPGYTFQWFVNGTPVGGSVIPVTGSGVYTLVVTAPNGCTGTNQIPITVL
                     SDQDLPNITDTDFQFSYMGVPFDEQDTILVCAPGTCMSGDLLTTWYVDGTPTVLTQPI
                     FAEYGTIGGCGSNNVYPNQGLFWNQVVQTDGWYPLHVHITLHVEGCGDDTLAFDVFDS
                     VYFHIAAPEIVPVGDVVLCLGDTLAVPVHCTGCTEMEITGPPGMYSVSPGQDTVLVWG
                     LGGFLVIGTNTQGAFCKDFISFQVVQAQPPPITVEPVLVCPGDTALLWTSFLSNQYDW
                     IGPDGPLQGNNDSIFVSMPGTYYLTAYTDQGCALFNGPAYVNQFATPSLSMQPDNVLC
                     PGETGALEILGGGITEVQWNPPFSGSGLVQLVDSAGTYSCLVTACGTQLELNAEVVSS
                     SVSVSLDPGPFSICGGGSVLLDGPTGDYTYFWVPGGGTTEDLLVFAPGDYTLQVTDGF
                     GCTATSDAATVLLQNFTQPLEATGDSICAGDTLVLSATGSGTITWYADEALTDPLATG
                     AAYIGGGQLASDTLYVTQTEGDCTGPALPVVVSVGPTPGTLVISGDTLLCEGDDLSLF
                     ASASGATFYQWATPQGTASTQSYTLNSIGVGASGAYVCLPLINACPGSPAAVVVLVQS
                     PPAVPVISGASALCEGEPLVLHVNGSSGEFTWYGPDGAAIGAGPDWSGVAQNGEATYT
                     VVADGGTCPDVQGSFTVLVDDCSIIIPNIFTPNGDGQNDQFILEGGATTAFHLRLFNR
                     WGQEVRFIQATQHLSWDGRDEDHQTLPDGVYYFELMELRSGQTIPHTGYIQLSRGR"
BASE COUNT          607 a          889 c          921 g          507 t
ORIGIN      
        1 cccacgtggc gcacccagta gggctgggcg caaacctgtt ccgctgcgct gaccgccacc
       61 aacagcagaa cagggacgag ataccgcgcg cgcatggtgg ggtgaaggta gccggtggtc
      121 cgatccatcc ggggtccagc gccacctcat gaacggggaa aggcccgtag gctcgacctc
      181 gccctatcgg cccctgctca actggatata cccggtatgc gggatggtct gtccgctgcg
      241 caattccatt aattcgaagt agtaaactcc gtcgggaagc gtctggtggt cttcatcacg
      301 gccatcccac gaaaggtgtt gcgtggcctg aatgaagcgc acctcctgcc cccagcgatt
      361 gaacaggcgc aaatggaacg cggtggtcgc gccaccctcc aggatgaact ggtcgttctg
      421 gccgtcgcca ttcggggtga agatattggg gatgatgatc gagcaatcgt ccaccagcac
      481 ggtgaagctg ccctgcacat ccggacaggt accgccatca gcgaccacgg tgtaggttgc
      541 ttcgccgttc tgtgccacac cggaccaatc gggaccagcg ccgatcgcgg caccatctgg
      601 gccgtaccag gtgaactcac cggaggaccc gttcacatgg agcacgaggg gttcaccttc
      661 gcacaaggcc gaggccccac tgatcacggg aacggccgga ggcgactgca ccagcaccac
      721 aaccgcagcc ggtgatccgg ggcaggcgtt gatcagtggc aagcacacat atgcgcccga
      781 ggcaccgacg ccgatactgt tcagcgtgta gctctgcgtg gaggccgttc cctggggtgt
      841 ggcccactga tagaacgtgg cgccggatgc tgaagcaaaa agcgacaggt catcgccttc
      901 gcacaggagc gtatcgccac tgatgaccag ggtgccgggg gtcggcccca cgctcaccac
      961 aacggggagc gccggcccgg tgcaatcgcc ttcggtctgg gtcacgtaca gggtatcgct
     1021 ggcgagttgc cctcccccaa tgtacgcagc tcctgtggcc aacggatcgg tgagtgcctc
     1081 gtccgcgtac caggtgatgg tgcccgaacc agtggcggag agcaccaacg tatcgcctgc
     1141 acagatcgag tcgcccgtcg cttccagtgg ctgtgtgaaa ttctgcaaga gcacggtcgc
     1201 tgcatcggaa gtggccgtac atccaaaacc atcggtcacc tgcagggtgt agtcgcccgg
     1261 cgcgaacacg agaagatcct cggtggtgcc ccctccgggt acccagaaat aggtgtagtc
     1321 accggtgggg ccgtccagta gcacgctacc gccaccacaa atggagaagg gaccgggatc
     1381 gagggaaacg gacacggaac tgctgacgac ctcggcgttc agctccagtt gcgttccaca
     1441 ggccgtaacc aagcagctat aggtcccggc ggaatcgacc agctgcacca gtccactgcc
     1501 cgagaatggc gggttccatt gcacctcggt gatcccgccg cccaggatct ccagcgcgcc
     1561 tgtttcgccg gggcagagca cattgtccgg ctgcatgctc aagctgggcg tggcgaactg
     1621 gttcacgtac gcgggcccgt tgaagagggc acagccttga tccgtgtagg ccgtcaggta
     1681 gtaggtgccc ggcatcgaaa cgaagatgct gtcgttgttc ccttgcagcg gtccgtccgg
     1741 cccgatccaa tcatactggt tcgagaggaa ggaggtccag agcaaggccg tatcgccggg
     1801 acagaccagt accggttcca cggtgatggg cggcggttga gcctggacca cctggaagga
     1861 gatgaagtcc ttgcagaagg ccccctgggt attggtaccg atcaccaaga aaccgccgag
     1921 accccacacc agcacggtgt cctgtccggg tgatacggag tacatcccgg gcggcccggt
     1981 gatctccatt tcggtgcatc cggtacaatg gaccggcaca gcgagggtat caccgaggca
     2041 cagcacgaca tcccccaccg gcacgatctc gggcgcagcg atgtggaagt agacgctatc
     2101 gaagacgtcg aacgcgagcg tgtcatcacc gcagccttcc acatgcaggg tgatatgtac
     2161 atgcaggggg taccagccat cggtctgtac cacctggttc cagaacagcc cctggttcgg
     2221 atagacgtta ttggacccac aaccaccaat tgtaccatat tccgcaaata tgggctgggt
     2281 gagcacggtg ggcgtcccat ccacgtacca ggtggtcagc agatcaccgg acatgcaggt
     2341 gccgggggca caaacgagga tcgtgtcctg ctcgtcgaat ggcacgccca tgtatgagaa
     2401 ttggaagtcc gtgtcggtga tgttgggcag gtcctgatca ctcagcacag tgatcgggat
     2461 ctggttggtg cccgtacaac cgttcggggc ggtgaccacc agggtgtaca caccggatcc
     2521 ggtcaccggt atcacggacc cgccgacagg ggtgccgttc acgaaccatt ggaacgtgta
     2581 gcccggctgc aggttgttcg tttccagggt ggtgggcact tcacaaatgg agaacacgtt
     2641 gaacggtccc ggaaaagcgg gagcaccacc cagcgatgcc caggctatcg gcgcagggag
     2701 cacagtgaga tggatggaat ccgtactggt gtagcatccg ttgctggcgg tctgggtgcg
     2761 ccagtaccag ccggtggacc aggcatgaat ggtatccata tccgatgcac cggtactcca
     2821 cacctcatcg acgttccacg agatcgtacg cgcaggatcg caaacgcaat acgggcaggc
     2881 cggccggtgg atctcatgtg gccagaagag cgtatcgccc ccgc
//
LOCUS       DAOQOC010000266         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18133_length_2924_cov_3.568142, whole genome shotgun sequence.
ACCESSION   DAOQOC010000266 DAOQOC010000000
VERSION     DAOQOC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18133_length_2924_cov_3.568142"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /gene="katG"
                     /locus_tag="PL106_05395"
     CDS             <1..1987
                     /gene="katG"
                     /locus_tag="PL106_05395"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNA32536.1"
                     /translation="MTDSQDWWPADWGHYGGLMIRMAWHIAGTYRTADGRGGAGTGNQ
                     RFAPINSWPDNGNLDKARRLLWPVKQKYGNSLSWADLYILAGNVALESMGFKTFGFGG
                     GREDIYAPEEDVYWGAEAEWLATSDKPNSRYSGDRDLENPLAAVQMGLIYVNPEGPDG
                     VPSAVASGRDIRETFKRMAMNDEETVALIAGGHTFGKMHGAGDARLVGPAPEAAPLEA
                     MGLGWINAFGKGKGNDTTTSGLEGAWKPNPTTWDDGYFRMLLDYDWDLVKSPAGANQW
                     EPVGLAEEDYMPSAHDPKKKVKPVMTTADLALRMDPIYAPISRRFRKDHKAFADAFAR
                     AWFKLTHRDMGPKCLYLGSEVPKEDLIWQDPIPAGSPLTDSDSTALKSKILASGLTVG
                     ELVSAAWASASTFRGSDKRGGANGARVRLAPQKDWAVNDPAQLAKVLKALEKIQDEFN
                     AGGKKVSMADLIVLGGNAAVEKAAKDAGHAITVPFTSGRGDASIEQTDVESFAVMEPQ
                     ADGFRNWQKKAYSVPAEELLIDRAQLLTLSAPEMTVLVGGLRVLGANTGGSKNGVFTE
                     RPGQLTNDFFVNLLDMGTTWTALSNGHTGTYEGRDRKTNAVKWTGTRVDLIFGSNSQL
                     RAISEVYAQNDAKEKFVKDFAKAWAKVMDLDRFDLKK"
     gene            2103..2537
                     /locus_tag="PL106_05400"
     CDS             2103..2537
                     /locus_tag="PL106_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32537.1"
                     /translation="MRTNDDILLMEGAHITMHVRPSERLMLQTWRGFVPSGTFRSIMD
                     RALVHAKRLDVQRWFNDLRKMEAILQKDEKWTMEDWFPRLAMTNVSRMAFLMSSDYFN
                     QRSVDRIMQVGTEMMPLSVGYFDDAEEARSWLLSSEPAMEPV"
     gene            complement(2597..>2924)
                     /locus_tag="PL106_05405"
     CDS             complement(2597..>2924)
                     /locus_tag="PL106_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNA32538.1"
                     /translation="SFIVGTAGLLLFLLITQRSVSWAGVREAPAHVWLGGLLGAFYVT
                     VIILAFPQLGPGLTFGLVVAGQMLLSLVLEHFNILVAQQSPINAMKVLGMALVVVGVI
                     IIRKYQ"
BASE COUNT          605 a          893 c          953 g          473 t
ORIGIN      
        1 gatgaccgac tcgcaggact ggtggcccgc cgactggggc cactacggcg gcctgatgat
       61 ccgcatggcc tggcacatcg cgggtaccta ccgcacagcc gatgggcgcg gcggcgcggg
      121 caccggcaac cagcgtttcg caccgatcaa cagctggccg gacaacggca acctcgacaa
      181 ggcacgccgc ctgctttggc ccgtgaagca gaagtacggc aacagcctca gctgggccga
      241 cctgtacatc ctggccggca acgtggcctt ggagagcatg ggcttcaaga ccttcggctt
      301 cggcggtggc cgcgaggaca tctacgcacc cgaggaggac gtgtactggg gcgcggaagc
      361 cgaatggctc gccaccagtg acaagcccaa cagccggtac agcggcgatc gcgatctgga
      421 gaacccgctg gcggccgtgc agatgggcct gatctacgtg aaccccgaag gccccgatgg
      481 tgtgcccagc gcagtggcct cgggtcgcga catccgcgag accttcaagc gcatggccat
      541 gaacgacgag gagaccgtgg cgctgatcgc cggaggccac accttcggca agatgcacgg
      601 tgcaggcgat gccaggctgg tgggacccgc gccagaagca gcgcctctgg aagcgatggg
      661 cctgggctgg atcaatgcgt tcggcaaggg caagggaaat gataccacca ccagcggcct
      721 cgaaggcgct tggaaaccga accccaccac gtgggacgac ggttacttcc gcatgctgct
      781 cgactatgac tgggacctgg tgaagagccc tgcgggcgcc aaccagtggg agccggtagg
      841 ccttgcggaa gaggactaca tgccctccgc gcacgatccc aagaagaagg tgaagcccgt
      901 gatgaccacg gccgacctcg ccctgcgcat ggaccccatc tacgcgccca tctcgcgccg
      961 tttccgcaaa gaccacaagg ccttcgccga tgccttcgcc cgcgcgtggt tcaagcttac
     1021 ccaccgcgac atggggccga agtgcctcta cctcggtagt gaagtgccga aggaggatct
     1081 gatctggcag gatcccattc cggcgggatc acccttgacc gattcggatt ccacggcact
     1141 gaagtccaag atcctcgcga gcggcctcac cgtgggtgaa ttggtgagcg cagcgtgggc
     1201 ttcggcctcc accttccgcg gtagcgataa acgcggtggc gccaacggcg cccgtgtgcg
     1261 cctggccccg cagaaggatt gggccgtgaa cgatcccgcg caactggcca aggtgctgaa
     1321 ggcactggag aagatccagg atgaattcaa cgcgggcggt aagaaagtgt ccatggccga
     1381 cctgatcgtg ctgggcggga acgcggcggt ggaaaaggcc gcgaaggatg ccggtcacgc
     1441 catcacggta ccttttacca gcggccgtgg tgatgcgagc attgagcaga ccgatgtgga
     1501 gagcttcgcg gtgatggagc cgcaggccga tggtttccgc aactggcaga agaaggccta
     1561 cagcgtaccc gccgaggaat tgctgatcga ccgggcccaa ctgctcaccc tgagcgcacc
     1621 ggaaatgacg gtgctcgtcg gcggcctgcg cgtgctgggc gctaacaccg gcggcagcaa
     1681 gaacggggtg ttcaccgaac gccccggcca gctcaccaac gatttcttcg tgaacctgct
     1741 ggacatgggc accacgtgga ccgcgctcag caacggccac accggtacct atgaaggtcg
     1801 ggaccgcaag accaatgcgg tgaagtggac cggcacacgg gtggacctga tcttcggcag
     1861 caactcgcaa ctgcgcgcca tcagcgaggt gtacgcgcag aacgacgcca aggagaagtt
     1921 cgtgaaggac ttcgcgaagg cctgggccaa ggtgatggac ctggaccgct tcgatctgaa
     1981 gaagtagatc gcttcgtttt gagatgaaga ggatggcccg ggaacgggcc atcctcactt
     2041 ccatacggtt cctgttgctt gtgtttgttc cggacccgtg gatcgcctac cttcacgaac
     2101 ccatgcgcac gaacgacgat atcctcctga tggaaggcgc ccacatcacc atgcatgtgc
     2161 gaccgtccga acgcctgatg ttgcagacct ggcgtggttt cgttcccagt gggacattcc
     2221 gttcgatcat ggaccgggca ctggtgcatg ccaagcgact tgatgtgcaa cgctggttca
     2281 atgacctgcg caagatggag gccattctcc agaaggacga gaaatggacc atggaggatt
     2341 ggttcccccg gctggccatg accaacgtgt cgcgcatggc cttcctgatg agctcggatt
     2401 acttcaacca acgcagcgtg gaccggatca tgcaggtggg cacggaaatg atgccgcttt
     2461 cggtgggcta tttcgatgat gccgaggaag cgcggtcgtg gctcctgagc agcgagcctg
     2521 cgatggaacc ggtataggtc cggcagtccc cgtcgtgacg ggcggagggg atatggctgg
     2581 acgctcgggg tggcggctat tgatactttc ggatgatgat cacgccgacg accaccagcg
     2641 ccatgcccag caccttcatc gcgttgatcg ggctttgctg ggccaccagg atgttgaagt
     2701 gctccagtac cagtgaaagc agcatctgcc ccgccaccac cagcccgaac gtgaggccgg
     2761 ggcccagttg cggaaaggcc aggatgatca cggtgacata gaacgccccg agcaggccgc
     2821 cgagccacac atgcgcgggc gcctcccgta cgccggccca ggagacgcta cgttgggtga
     2881 tcaacagaaa gagcagtaga ccggcggtac ccacgatgaa ggaa
//
LOCUS       DAOQOC010000267         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18179_length_2920_cov_3.450960, whole genome shotgun sequence.
ACCESSION   DAOQOC010000267 DAOQOC010000000
VERSION     DAOQOC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18179_length_2920_cov_3.450960"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PL106_05410"
     CDS             complement(<1..1016)
                     /locus_tag="PL106_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HNA32539.1"
                     /translation="MITFLILAAFVLGLLAVTRLSRVYELTAELRGKREEEISDRETK
                     MNARLLWAFPFVYFSSFLWLTLHYAKAMLPVSASEHGVWLDDLMNFNWVLLIVAFFIT
                     NVLLFYFTGKYYHQADRRAYYYPHNNKWELVWTVVPAVVLMSIIIYGLTVWNRITGPA
                     TPGTPEVELYAQQFKWLARYPGPDGRLGPTDFRLINATNELGIVTPTSISARLTELAE
                     ETAAAQKRLATEQDQLPDEKVQELKDRIKHLERMSGKIINLRTLMNNDIKEKGAESPY
                     KHGSDDLVLKEFHLPVNEDVRLVIRSKDVIHSAFIPHMRMQMNAVPGMSTVFRMKPTI
                     TTDS"
     gene            complement(1047..2267)
                     /locus_tag="PL106_05415"
     CDS             complement(1047..2267)
                     /locus_tag="PL106_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HNA32540.1"
                     /translation="MNFTLSNRARTLSMALMGLGLVVAGIGFVTTRSHDAAHAGQHFW
                     SALFLNGFFFFGISLGALFFYSLQWATETAWSVLVRRVYEGIVGFLPIGAAVIVICLL
                     AGQFHLNHIWHWMDHRVYDPADLEHYDPIIAGKSAYLNAPFFWLRVVLFMGTFIFAAN
                     WFRKQSLKMDQLTGDDLRTQHLLTYRRSALFLVFFAVFSSVLSWDWLMSIDTHWFSTL
                     YGWYIFAGMWCTAMVTGVIVVLYLKSKGYLPQVNNNHIHDMGKWVFATSFLWSYLWFS
                     QFMLYWYADIPEEVAYAAVRIEHHPGLMWGMFFINFAVPMVLLMSRDAKRNPRFLIGV
                     GTVILIGHWLNVCQLVLPGALGENFHQVGALEAGLGLFFLGLFVLIVLRTLTKAPLTV
                     VNSPYLEESVHHSI"
     gene            complement(2283..>2920)
                     /locus_tag="PL106_05420"
     CDS             complement(2283..>2920)
                     /locus_tag="PL106_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNA32541.1"
                     /translation="IIVLGAALLASCAADNGPGIEYMPDMYRGPAVEAYVDYGQDPYY
                     FGEDKAQEQRNTPSARTAPVGTIPWSADPAMAAYNFPYPYTNDTTGYGRAGRELFSPI
                     PFTQENVDKGKIIYDKFCLHCHGAKGDGDGKVVEVGGHPPPTAYNGPLKNLPEGKMFH
                     TITYGKGMMGSHAGQLDKEERWTVIQYVKYLQRGGKMTDAVEAPVDSTANK"
BASE COUNT          569 a          868 c          899 g          584 t
ORIGIN      
        1 atactgtccg tggtgatcgt cggcttcatc ctgaacacgg tgctcatgcc gggtaccgcg
       61 ttcatctgca tgcgcatgtg cggaatgaac gcgctgtgga tcacatcctt gctccggatc
      121 accagtcgca cgtcctcgtt caccgggagg tggaattcct tgagcacgag gtcgtcggag
      181 ccatgcttgt acgggctctc ggctcctttc tccttgatgt cgttgttcat cagcgtgcgc
      241 aggtttatga tcttgccgga catgcgctcc aagtgcttga tgcggtcctt caattcctgc
      301 accttctcgt cgggcaactg gtcctgctcc gtggccagac gcttctgcgc agctgcggtc
      361 tcctcggcca gttcggtcag tcgggcgctg atcgaggtcg gcgtgacgat gcccaattcg
      421 ttggtggcgt tgatcagccg gaaatcggtg gggcccaggc gaccgtccgg acccgggtag
      481 cgcgccagcc acttgaactg ttgggcgtac agttccacct ccggggtgcc gggggtggcg
      541 ggtccggtga tccggttcca taccgtgagc ccataaatga tgatgctcat cagtaccacg
      601 gccggaacga ccgtccagac cagctcccac ttgttgttgt gcgggtagta ataggcccga
      661 cggtccgctt gatggtagta ttttccggtg aagtagaaga gcaggacgtt cgtgatgaag
      721 aacgccacga tcaggagtac ccagttgaag ttcatcaggt cgtccagcca cactccgtgt
      781 tcactggccg aaacgggcag catcgccttg gcgtaatgca gggtgagcca caggaaagaa
      841 ctgaagtaaa cgaaggggaa ggcccacagc agacgcgcgt tcatcttggt ctcgcggtcg
      901 ctgatctcct cctcgcgctt gccgcgcaac tcggccgtga gttcatacac gcggctcaga
      961 cgcgtcacgg ccagcagacc gagcacgaag gcagcgagga tcaggaaggt gatcatggga
     1021 tcggtgtgtc gatgcggttg ttccggtcag atgctgtggt gtacgctctc ttcaaggtac
     1081 ggactgttca ccacggtgag cggggccttg gtaagggtgc gcaacacgat gagcacgaag
     1141 agaccgagga agaaaaggcc caggccggcc tccaacgcgc ccacctggtg gaaattctca
     1201 cccagtgcgc caggcagcac cagctggcaa acgttcagcc agtggccgat caggatcacc
     1261 gtgcccaccc cgatcaggaa gcggggattg cgcttggcgt cacggctcat cagcaacacc
     1321 atcggcaccg cgaaattgat gaagaacatg ccccacatca ggccggggtg gtgttcgatt
     1381 cgtaccgccg cgtaggccac ctcctcggga atgtccgcgt accagtacag catgaactgg
     1441 ctgaaccaga ggtagctcca aaggaagctc gtggcgaaca cccatttgcc catgtcgtgg
     1501 atgtggttgt tgttcacttg cggcaggtag cctttgctct tcaagtagag caccacgatc
     1561 accccggtga ccatggccgt gcaccacatg ccagcgaaaa tgtaccagcc gtacagtgtg
     1621 ctgaaccagt gcgtgtcgat gctcatgagc caatcccacg acaggacgct gctgaacacg
     1681 gcgaaaaaga ccaggaacag tgcggaccgg cggtaggtga gcaggtgctg cgtccgcaga
     1741 tcgtcaccgg tcaactggtc cattttcaaa ctctgcttgc ggaaccagtt ggccgcgaag
     1801 atgaaggtgc ccatgaacag gaccacccgc aaccagaaga agggcgcgtt gaggtaggcg
     1861 cttttccccg cgatgatggg atcgtaatgc tcaaggtccg ccggatcgta tacccggtga
     1921 tccatccaat gccagatgtg attgagatgg aactggcccg ccagcaggca gatcacgatg
     1981 accgccgcac caatgggcag gaagcccacg atgccctcgt acacacgacg tacgagcacg
     2041 ctccatgctg tttcggtggc ccactgcaac gaatagaaga acagggcgcc gaggctgatg
     2101 ccgaagaaga agaatccgtt gaggaagagg gccgaccaga agtgctggcc cgcgtgcgcc
     2161 gcatcatgtg aacgggtggt cacgaaaccg atccccgcga ccaccaggcc cagacccatg
     2221 agcgccatgc tcagcgtgcg cgccctattg ctcaatgtga agttcatcgg tgctctatcg
     2281 gatcatttgt tcgccgtgct gtcgacaggg gcctcgaccg cgtccgtcat cttgccgccg
     2341 cgttgcaggt acttcacgta ctggatcacc gtccagcgct cctccttgtc caactgaccg
     2401 gcgtggcttc ccatcatgcc cttgccgtag gtgatggtat ggaacatctt cccttcgggc
     2461 aggttcttga gcgggccgtt gtacgccgtt gggggcgggt ggccacccac ttccaccacc
     2521 ttgccgtcgc cgtcgccttt cgcgccatgg cagtgcaggc agaatttgtc gtagatgatc
     2581 ttgcccttgt ccacgttctc ctgggtgaag gggatcggac tgaacagttc acgaccggca
     2641 cgaccgtagc cggtcgtatc gttcgtgtac ggatagggga agttgtatgc cgccatggcc
     2701 gggtccgcgc tccatgggat ggtgcccacc ggtgcggtcc gggcgctggg cgtgttgcgc
     2761 tgctcctggg ccttgtcctc cccgaaatag tacgggtcct gcccgtaatc cacataggcc
     2821 tccacggccg gaccccggta catgtccggc atgtattcga tgccgggccc gttatccgcc
     2881 gcgcaggagg cgagcagtgc ggcgccgagg acgatgatgg
//
LOCUS       DAOQOC010000268         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18180_length_2920_cov_3.075742, whole genome shotgun sequence.
ACCESSION   DAOQOC010000268 DAOQOC010000000
VERSION     DAOQOC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18180_length_2920_cov_3.075742"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PL106_05425"
     CDS             <1..510
                     /locus_tag="PL106_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32542.1"
                     /translation="LTMPGGTGERILGRMTAYLMVCVAQGILLILIGIWFLPLIGLPQ
                     LRVAGPSMIVLLFIAAVPTALAATSLGVLVGSMSRSQQQSAVLGSTFVVIMSAIGGIW
                     VPLYIMPPAMRALGQLSPLHWGLEAFNVPLLREGDLWELLPSVAPLLLFSVVCIIAAI
                     VAERRATYR"
     gene            507..1715
                     /locus_tag="PL106_05430"
     CDS             507..1715
                     /locus_tag="PL106_05430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNA32543.1"
                     /translation="MRQDAVHVTGMGMVTAIGVGVEANLASLLAERTGIGPITRLVTR
                     HRGKIPAAEVRMGDMELSAIAAPADTRGWTRAALLGLAAVREATLQAGLDPRGPRIGL
                     ISATTAGGIDKSEGIYDRFFDNAPSDEVLEYIGTHDPGEHAERIAHELGMSSVVTTVS
                     TACSSSANAIGFAARLIRAGLLDAAIAGGTDALNKFTLNGFNSLMILDPQACRPFDGS
                     RAGLNLGEAGAYVVLESATHARRRGAAPLALVNGFANTNEAYHATASSPDGEGARKAM
                     DRALASAGLRPQDISHVNTHGTGTLNNDASEGLAMHRLFEGKVPPFTSTKSFTGHTLG
                     AAGAVEAIYAVLAIRAGAHFATLRHGSLLVEAPVVPILRSSSGHAITHVLSNSFGFGG
                     NNTSLILSAP"
     gene            1712..2770
                     /locus_tag="PL106_05435"
     CDS             1712..2770
                     /locus_tag="PL106_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNA32544.1"
                     /translation="MKADVHIVGASMIGPQPGFGRDALERVVAYDTHPMKAMEPDLSG
                     YVDPMRGRRMAKVARISLGCGLQALERAGVARPEAIIVGTGLGCMESTERFFASVLQQ
                     DEEAPSPVAFIQSTHNNVAGQLALAVKCTGYNFTYLHRGLSFTSALLDAWLQVGREGA
                     RNVLAGGAEVITDDYFITQRHTGIWKPAIKSNLSVLNSTDTGALCGEGAAFFVLDNVP
                     RGKDSVRIRDVDISWRGDAKGIVPHVKDFIRRNGLAPGDIDLVMLGPNGDAVQDRGYD
                     PTAALLPQATVATFKPLCGEYYAANAFGLWLCRSAMVTGGLPPEVVLHGSARGDFRRV
                     LLYDHYQLKDHSLVLLER"
BASE COUNT          512 a          943 c          926 g          539 t
ORIGIN      
        1 ctcaccatgc ccggcggcac cggggagcgc atacttggcc gcatgaccgc ttacctgatg
       61 gtgtgcgtag cgcagggcat tctgctgatc ctgatcggga tatggttcct gccgttgatc
      121 gggctgccgc agctgcgcgt agccggtccg tccatgatcg ttctgctgtt catcgccgcc
      181 gtccccactg cacttgcggc cacttcgctc ggcgtgctgg tgggctccat gagccgcagc
      241 cagcagcagt cagccgttct cggatccacg ttcgtggtga tcatgtcggc catcggaggc
      301 atctgggtcc ccttgtacat catgccgccg gcgatgcgcg cactggggca gctgtcgccg
      361 ttgcattggg gactggaagc cttcaatgta cccttgctgc gcgaaggcga cctgtgggag
      421 ttgctgccca gcgtggcacc attgctgctt ttttccgtcg tttgcatcat cgcggccatc
      481 gtcgccgaac ggagagccac ctaccgatga gacaggatgc ggtccatgtg accggcatgg
      541 gcatggtaac ggccatcggc gttggtgtcg aggccaacct ggcatccttg ctcgcggagc
      601 ggaccggcat cggccccatc acacggctgg tcacgcgtca tcggggaaag atccctgccg
      661 ccgaggtgcg gatgggcgac atggaactgt ccgcgatcgc cgcacccgcc gatacgcgcg
      721 gctggacacg tgccgccttg ctgggcctgg ccgcggtacg cgaggcgacg ttgcaggcag
      781 gcctcgaccc acgcggtccg cggatcggcc tcatctccgc caccacggcg ggcggcatcg
      841 acaagagtga gggcatctac gaccggttct tcgataatgc cccctcagac gaggtattgg
      901 agtacatcgg cacgcacgac cccggcgaac acgcggagcg catcgcacac gagttgggca
      961 tgagcagcgt ggtgaccacg gtgagcacgg cctgttcctc ctcggccaac gcgatcggct
     1021 tcgccgcacg cctcattcgg gctggtctgt tggatgcggc catagccggt ggtacagatg
     1081 cgctgaacaa attcaccctc aacggcttca acagtctgat gatcctggat ccgcaggcgt
     1141 gcaggccatt cgacgggtcc cgggcagggc tcaacctcgg cgaagccggg gcttacgttg
     1201 tgcttgaaag cgcgacacac gcacggcggc gtggtgcggc gcctctggcc cttgtcaacg
     1261 ggttcgccaa taccaacgag gcttaccatg ctaccgcttc atcacccgat ggggaaggcg
     1321 cacgtaaggc catggaccgg gcgctcgcat cagccggtct gcgcccacag gacatcagcc
     1381 acgtgaacac gcacggcacc ggcacgctga acaacgatgc ctccgaaggg ctggccatgc
     1441 accgcctgtt cgaagggaag gtacctccgt tcaccagtac gaaatcgttc accgggcata
     1501 cgctgggcgc tgccggcgcg gtggaggcca tctacgccgt gctggcgatc cgggcaggtg
     1561 cgcatttcgc cacgctgcgt cacggctccc tgctggtcga agctcccgtg gtaccgatcc
     1621 tccgttcctc cagcgggcat gccatcaccc atgtactctc caattcattc ggattcggag
     1681 gtaacaatac ttcactcatc ctatcagccc cgtgaaagcg gatgtgcaca tcgtcggcgc
     1741 gagtatgatc gggccgcaac ccggtttcgg tcgcgatgcc ctggaacgcg tggtcgccta
     1801 tgacacccat ccgatgaagg cgatggagcc cgacctgtcg ggttacgtgg accccatgcg
     1861 cggtcgtcgc atggccaagg tggcccggat cagtctgggg tgcgggctcc aggcactgga
     1921 acgtgcaggt gtggcaagac cggaggccat catcgtgggc acgggcctcg gctgcatgga
     1981 aagcaccgag cgctttttcg cttcggtcct tcagcaggat gaggaagcac cgagcccggt
     2041 cgccttcatc cagagcacgc acaacaacgt ggcgggtcaa ctggccctcg ccgtaaaatg
     2101 caccgggtac aatttcacgt acctgcaccg cggtctctcg ttcaccagtg cgttactgga
     2161 cgcgtggttg caagtggggc gcgagggggc ccgcaacgtg ctggccggtg gcgccgaggt
     2221 gatcacggac gattacttca tcacccaacg tcatacaggg atatggaaac ccgcgatcaa
     2281 gagcaacctg agcgtgctca acagtacgga caccggcgca ctgtgcggtg aaggcgcggc
     2341 cttcttcgtg ctggacaacg tccctcgtgg aaaggacagc gtgcggatcc gcgatgtgga
     2401 catctcttgg cgcggtgatg ccaaaggtat cgtaccacat gtcaaggatt tcatccggcg
     2461 gaatggactt gcgccagggg acatcgacct ggtcatgttg gggcccaatg gcgatgcggt
     2521 gcaggatcgt gggtacgacc ccaccgccgc gctgctcccg caggccacgg tggccacctt
     2581 caaaccgctg tgtggcgaat actacgcggc gaatgccttc ggtctctggc tctgccgctc
     2641 ggccatggta acgggcggcc tgccacctga agtggtgctg cacggatcag cacgcggcga
     2701 cttccgtcgc gtactccttt acgatcacta ccagctgaag gaccactcgc tggtgctgct
     2761 ggagcggtga ggggcgcacg gtaggccaac tattcctacc caccctcccg cagggtctgc
     2821 gctccctggc tgccgaagcg cctggcgaag gcatgctcgc gaccctgcgg catcccgccc
     2881 taccccatgt accgctcccg gatgaaccgg tggtagaaac
//
LOCUS       DAOQOC010000269         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18228_length_2916_cov_3.202726, whole genome shotgun sequence.
ACCESSION   DAOQOC010000269 DAOQOC010000000
VERSION     DAOQOC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18228_length_2916_cov_3.202726"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PL106_05440"
     CDS             complement(<1..325)
                     /locus_tag="PL106_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997827.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNA32545.1"
                     /translation="MNASDSEVVVGILAQEGYALSTTPEGADLVLLNTCSIREKAEYT
                     VLQRINEMKHYKHANPDLKVGVLGCMAERMRGELLEKKKVVDLVVGPDAYRSLPSLLH
                     DVEGGQ"
     gene            complement(443..1276)
                     /locus_tag="PL106_05445"
     CDS             complement(443..1276)
                     /locus_tag="PL106_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32546.1"
                     /translation="MSTRPRPHASASVSFIAVLLTCLMIVRTTTIKAQDDAGVQAHIQ
                     RIDAWIAQAEGEKNQEAVDCLRRDRATWVEFQQAIERSDAFRMLALSEAMKHPCPYPP
                     PGQDHTVVAAPAQPEEQQEGPEIIGQVYSMPSEGEYLPLEREEGDRSSARGGFIGYYS
                     SRTKSIRIEGVRSSVRYKQGQHRFVVKVWPGMDPFDLVKLYRFEVIEKKNERVVETSK
                     SSRSRYHSYSTDNTEGRVRVSFKKLAGQGVYEIVHEEPVPPGEYAFLSGEKVFAFGVD
                     E"
     gene            complement(1460..2089)
                     /locus_tag="PL106_05450"
     CDS             complement(1460..2089)
                     /locus_tag="PL106_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32547.1"
                     /translation="MSTTCLSRNTMAVALLLATSFVANGQAFEKGTSVVSLGLGITNY
                     PVGYTYTYGYGYYYDYTYDAGIAPALSANYEMGVAKLGPDHLGIGATVQFSGKKHEER
                     YLYSTVDWRSSYIAFGVRANYHFNWFHHIDKLDLYAGVLAGGGIGNSKWKVTSNSGGA
                     LLSENNDSKFRPHLDVLIGARYYFTDVIGVYCEFGELNTYANLGLSLKF"
     gene            complement(2215..2826)
                     /locus_tag="PL106_05455"
     CDS             complement(2215..2826)
                     /locus_tag="PL106_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32548.1"
                     /translation="MEPQGPFQSPHPPGGNMFTDFAKPYIGIIDNGALYRKPFVILYT
                     ALAALNLLSILGVLGFMFKGGIALIITGLFMIFALWIGFQLWWDRRTKVNSFATPGSE
                     FVALPVFSHLVQTMGEWTGTFVAIAGTGASLGGLIGSIGSGGGGGYGNPLAQFSNLGF
                     AGLIACPIVGFLILILSRAIAEQIRALVAVANNTRNIERNTAR"
BASE COUNT          550 a          868 c          906 g          592 t
ORIGIN      
        1 tctgcccgcc ttccacgtcg tgcagcagtg agggcaggct gcggtaggca tcggggccca
       61 ccaccaggtc caccactttc ttcttttcca gcaactcgcc acgcatgcgc tcggccatgc
      121 agcccagcac ccccaccttc agatccgggt tggcgtgctt gtagtgcttc atctcgttga
      181 tgcgctgcag caccgtgtac tcggcctttt cgcggatgct gcaggtgttc agaagcacca
      241 gatcggcccc ttccggcgtg gtggaaagcg catagccctc ctgggccagg atgcccacga
      301 cgacctcgct gtcgctcgcg ttcatctgac agccgtaact ctcgatatag accttcttgc
      361 tcacggggcg cgaaggtacc ggatccgctc cgtgctgaca gcctgtcaga ccggatgccc
      421 ctgccctcca gaacgcggat ggtcactcgt ccaccccgaa ggcgaagacc ttctcgccac
      481 tgaggaaggc gtactccccg ggtggaacgg gctcctcatg aacgatctcg tacaccccct
      541 gcccggccag cttcttgaag gagacccgta cacggccctc ggtgttgtcg gtgctgtacg
      601 aatgataccg gctgcggctg ctttttgagg tttccaccac ccgttcgttc ttcttctcga
      661 tgacctcgaa ccggtagagc ttcaccagat cgaaggggtc catgccgggc cacaccttca
      721 ccacgaaacg atgctgacct tgtttgtacc gcaccgagga acggacacct tcgatgcgaa
      781 tggacttcgt acgcgaactg tagtacccga taaagcctcc ccgtgccgaa gagcggtccc
      841 cttcttcgcg ttccaatggc aggtactcgc cctcgctggg catggaatag acctggccga
      901 tgatctcggg cccttcctgc tgctcttcgg gttgtgccgg ggctgctacc acggtgtggt
      961 cctggccggg gggcggatag gggcacgggt gcttcatggc ttcggaaagg gcgagcatcc
     1021 ggaacgcgtc gctccgttcg atggcctgct ggaattccac ccaggtggcc cgatcgcgac
     1081 gcaggcaatc caccgcttcc tggttcttct cgccttcggc ctgtgcgatc cacgcatcga
     1141 tccgctggat gtgggcctgc acccccgcat cgtcctgggc cttgatggtg gtggtccgca
     1201 cgatcatgag gcaggtcagc agcactgcga tgaatgatac cgatgcggaa gcgtgaggac
     1261 ggggtcgtgt gctcatttca tggctctgtt gatggggcaa aggtgctctg cggcccacga
     1321 gcaaggtagc gcgatcgcgc cattgacagg cctcaatgag tgtgtcccgc tccacgggca
     1381 gtagcatgtc cggcacgggc cgaagcaagc gccccggtga tccgcacagg accaccgggg
     1441 cgaataatgg ttggaccgat cagaacttca gcgacagacc gaggttggcg tacgtattga
     1501 gttccccgaa ctcgcagtac acgccgatga catcggtgaa gtagtagcgg gcaccgatca
     1561 acacatccag gtggggcctg aacttgctgt cgttgttctc actcagcaat gctccgcccg
     1621 agttgctggt caccttccac ttcgagttgc cgatcccacc accggccagc accccggcgt
     1681 acaggtcgag cttgtcgatg tggtggaacc agttgaagtg gtagttggcc cgcaccccga
     1741 aggcaatgta cgaggaccgc cagtccaccg tggaatacag gtatctttcc tcatgcttct
     1801 tgccactgaa ctggaccgtg gcaccgatgc ccaggtgatc cggccccagc ttggcgacac
     1861 ccatttcgta attggcgctc aaggccggcg cgataccagc atcatacgtg taatcgtaat
     1921 agtagccgta accgtacgtg taggtgtaac cgacgggata attggtgata cccaagccca
     1981 ggctcaccac actggtccct ttttcgaaag cttggccgtt ggcgacaaaa gaagtggcca
     2041 gcaacagcgc gacagccatc gtgttgcgtg aaagacatgt tgtgctcatc gttcgtgtgt
     2101 tcttggatca ggggttcgtt ctgcgaatgc gggccgaagg taggtgtggc atcctgaagg
     2161 ggaagcccga tcgagttttt accatgccgg agtgagcata tcccgatcgg gtggtcaccg
     2221 cgcggtgttc cgttcgatat tcctggtgtt gttggccacg gctaccagag cccggatctg
     2281 ctcggcgatg gcgcgcgaaa ggatcaggat gagaaagccg acgatgggac aggcgatcaa
     2341 gcctgcgaag cccaggttgc tgaattgcgc cagcgggttg ccataaccgc caccacctcc
     2401 agagccgata ctgccgatga ggcctcccag cgaagcgccc gtgcccgcga tggccacaaa
     2461 ggtcccggtc cattcgccca tggtctggac caggtgggaa aagaccggca aggccacgaa
     2521 ctcggagccc ggtgtagcaa aggagttcac cttggtgcgc cggtcccacc agagctggaa
     2581 tccgatccac aaggcgaaga tcatgaagag acccgtgatg atcagcgcga tgcccccctt
     2641 gaacatgaag cccagcacac ccaggatgct caacaggttg agtgccgcga gcgcggtgta
     2701 caggatgacg aagggtttgc ggtacagtgc gccgttgtcg atgatgccga tgtagggttt
     2761 tgcaaagtcg gtgaacatgt tgccaccagg agggtgcggc gactggaagg gtccttgtgg
     2821 ttccatgcga tcctggttgt gaggtggtgg cggtggcgaa ggaggtggtg gcggaggtgg
     2881 cggtggtggc ggtggtggcg agggtggtgg tgcctg
//
LOCUS       DAOQOC010000270         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18231_length_2916_cov_2.394967, whole genome shotgun sequence.
ACCESSION   DAOQOC010000270 DAOQOC010000000
VERSION     DAOQOC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18231_length_2916_cov_2.394967"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PL106_05460"
     CDS             complement(<1..405)
                     /locus_tag="PL106_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32549.1"
                     /translation="MRVEQLVVGASFSFLAMDCAAQVADAGPDTLLCEGAYTMQGSAV
                     PVGGTGTWTLLMGCATIIDPNAPSSGITGLCPGENVFVWEVFDGFATTSDTVVIGVVD
                     LPMIADAGPDQTIIAPPFSAQLMAKEGDWAQVR"
     gene            complement(529..1404)
                     /locus_tag="PL106_05465"
     CDS             complement(529..1404)
                     /locus_tag="PL106_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA32550.1"
                     /translation="MDPRQPSWLDRNEFPFATHTFAHPDGRMHYVDEGEGPTILFVHG
                     TPDWSFGFRHLIKAFRSTHRCVAVDHLGFGLSDKPRLADLTVQAHARRLQAFIDNLGL
                     QDVTLVVTDFGGGIGLQHALERPGNVKRIVLYNTWMWALNDDERFARPARMMRGWLGR
                     LAYLRFNFSVNVMMPTAYGDRKVLTPALHTQYKQVFPTPASREPLHKLVKEMLDAGPF
                     WAAQWKEVDRLRTIPTLIAWGMKDRFFPPDLLEKWRQALPHATVRTFPEAGHFVHEEA
                     PQELVQAIRTFLIEH"
     gene            complement(1459..2046)
                     /locus_tag="PL106_05470"
     CDS             complement(1459..2046)
                     /locus_tag="PL106_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNA32551.1"
                     /translation="MNLANDHRAVRDYLQLFHPLEDGPWNAMVAAFRTRTYDRGQVIT
                     GVGDVQRDLLFILEGVQYSYFHKDDKSYVMAFTYPVGISGIPESFLSQLPSTYSLETI
                     SETRALALSHSDLQRLYDAHPAIERLFRKITEAMLIASAQGRYRQVSTSAEERFRQFA
                     ALSPHLFQLVPHKLLASYLGIHPTNFSKLYNAVRI"
     gene            complement(2049..2732)
                     /locus_tag="PL106_05475"
     CDS             complement(2049..2732)
                     /locus_tag="PL106_05475"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNA32552.1"
                     /translation="MEPIPLLSSFYFGSVEHYRLLAQHHRVVIDVGEHYERQSYRTRT
                     SIVGPNGVQHLNVQIARDHGNKMPLRTVGLSYVESWPQQHLHAIRSAYGQTPWFIHYI
                     DDIEDVVLRKYDRLVDLDLATLALGMKWLGVTTEVLVSEQYVEGDSYHVTAGGSGPVT
                     PSTPLEERSPLLDLRTSFHPKRTLPSEVSPCPPYPQVFADRHGFVPRLSVIDLVCNCG
                     PRSLVHLRT"
BASE COUNT          566 a          850 c          927 g          573 t
ORIGIN      
        1 acggacctgc gcccagtcgc cttccttggc catcaactgc gcggagaacg gtggtgctat
       61 gatggtctgg tccggaccgg cgtctgcgat catgggcagg tccaccactc cgatcacgac
      121 ggtgtcactc gtggttgcga agccatcgaa cacctcccat acgaatacat tttcgcctgg
      181 acacaggccg gtgatcccag aacttggtgc gttcggatcg atgatcgtgg cacaacccat
      241 gagcaaggtc catgttccgg ttccgcctac cggcacagca ctgccttgca tggtgtaggc
      301 gccttcacaa agcagggtat ccggccccgc atcggccacc tgcgccgcgc agtccatcgc
      361 caggaacgag aaggaagcgc caactacaag ctgttccaca cgcatattcc ggaaggtttt
      421 ccgaaaggaa gcaggttgcg gttctcgtac cgccggggtc aacgtgaaag atccccgacc
      481 tgggtgttgg agatcggtat agcgaagcgg cccgatcaca caccgggatc agtgttcgat
      541 caggaatgtg cggatggcct ggacaagttc ctgtggggcc tcctcatgga caaagtgccc
      601 ggcctccggg aaggttcgta cagtggcgtg cgggagcgcc tgccgccatt tctccagcag
      661 gtccggcggg aagaagcggt ccttcatgcc ccaggcgatc agcgtgggga tcgtacgcag
      721 ccggtccacc tctttccatt gcgccgccca gaacggacca gcgtcgagca tctccttcac
      781 cagcttatgc aagggctcgc gtgaggctgg ggtggggaag acctgcttgt actgcgtatg
      841 caacgctggt gtcagcacct tcctgtcacc ataggctgtg ggcatcataa cgttcacgct
      901 gaagttgaag cgcaggtagg ccaggcgccc cagccagccg cgcatcatcc gcgctggccg
      961 cgcgaaacgc tcatcgtcgt tcaaggccca catccatgtg ttgtagagca cgatccgctt
     1021 aacgttcccc ggacgctcca gcgcatgctg cagcccgata cccccgccga agtccgtcac
     1081 caccagcgtc acatcctgca gcccaaggtt gtcgatgaag gcctgtagtc ttcgggcgtg
     1141 tgcctgcacg gtgagatccg ccaagcgtgg cttatcgctc aggccgaaac ccaggtgatc
     1201 caccgctaca cagcggtgcg tgctgcggaa ggccttgatc aggtggcgga agccgaaact
     1261 ccaatcaggc gtgccgtgga cgaagaggat ggtcggaccc tcgccttcat ccacgtaatg
     1321 catgcggcca tcgggatggg cgaaggtgtg tgtggcgaac gggaactcgt tacggtcgag
     1381 ccaggatggt tgtcggggat ccatggcagc aaagctccac cacgggctga cgcgcgttct
     1441 tggcgaaaac caacttcttc agatccggac ggcgttgtac agcttgctga aattggtggg
     1501 gtggataccg aggtagctcg cgaggagctt gtgcggaacg agttggaaca ggtgcgggct
     1561 cagtgcggcg aactgccgga atcgttcctc cgcactggtg ctcacctggc gatagcgccc
     1621 ctgcgccgag gcgatcagca tggcctccgt gatcttcctg aacaaccgct cgatggcggg
     1681 atgcgcgtca tagagccgtt gcaggtcgga gtgagatagt gcgagcgccc gtgtttcgct
     1741 gatggtctcc agcgagtagg tgctggggag ttgcgacagg aacgactcag gtatgccgct
     1801 gatgcctacc ggataggtga aggccatcac gtacgacttg tcatccttat ggaagtagct
     1861 gtattgtaca ccttcgagga tgaacagcag gtcgcgttgc acgtcgccca cgccggtgat
     1921 cacctggccg cgatcgtatg tcctcgttcg gaacgcggcg accatcgcgt tccacggccc
     1981 atcctccaga gggtggaaga gctgcaggta gtcccgcacg gcgcgatgat cgttcgcaag
     2041 gttcatgatc aggtacgaag atgcaccagg gaccgcggcc cgcagttgca caccaggtcg
     2101 atcacgctca gacgaggaac gaagccgtgc cggtccgcga acacctgcgg atagggtggg
     2161 caggggctta cttcggaagg cagcgtgcgt ttggggtgga aggaagtgcg caagtccagc
     2221 aggggagacc gttcctccaa tggcgtggaa ggcgtcaccg gaccggaacc tccggctgtc
     2281 acgtggtagg agtcgccctc tacgtactgt tcggagacca acacttcggt agtgaccccc
     2341 agccacttca tgcccaaggc aagagtggcg aggtccagat ccaccaaacg atcgtacttc
     2401 cggagcacga cgtcttcaat gtcgtcgatg tagtggatga accagggtgt ttgcccatac
     2461 gcgctgcgga tggcatgcag gtgctgctgt ggccagcttt caacgtagct gaggcctacg
     2521 gtgcgcaagg gcatcttgtt gccgtggtcc cgggctatct gcacgttgag gtgttgcacg
     2581 ccgttcgggc caacgatgct ggtccgcgtc cggtagctct ggcgctcgta gtgctcgcca
     2641 acgtcaatga ccacgcgatg gtgttgcgcc agcaggcggt agtgttccac gctgccgaag
     2701 tagaaggagg agaggagcgg gatgggttcc aagggtgcga agatggatcg gtattttggc
     2761 cgacatgaag cttattgtcc gacacgcacc cctgttcgca ttgttcgtcg cttgcggtga
     2821 tcccccgtcc gatacgggca aggtcgttgc tccggacagt ccttcggtga ttgtggatac
     2881 cccgaaactc gcagcgaact ggcccggtta ttattc
//
LOCUS       DAOQOC010000271         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18368_length_2902_cov_3.768528, whole genome shotgun sequence.
ACCESSION   DAOQOC010000271 DAOQOC010000000
VERSION     DAOQOC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18368_length_2902_cov_3.768528"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2902)
                     /locus_tag="PL106_05480"
     CDS             complement(<1..>2902)
                     /locus_tag="PL106_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNA32553.1"
                     /translation="VLGSTVIPNGSGDDLFVAGFTDPNYLAFANDPSRFAQPGPFCEA
                     TAPIDLNAFLVDYASGVASSLSVSDPGNAINAPDNSGAVFTAPGAELVLDLGDTIQAG
                     ESVRVWWRSQTNGVPAQLLISSSLDQITYGAPQTWSTSSTTWNSVIYTVPSTARFIRL
                     QRAGSVSFTTLQVDALRYLNNTLSGGTWSGGPHVTATGIFTPTGPGSYPVSYTVASGA
                     CTYSSNKIIQVDPNAVGGSISGGGIYCPSSTGILILSGHTGIVQRWQSSPDGTLWTNI
                     PNTTTTLPWSSIGGTLQFRAQLKSGACGMATSSVATLVVEDTTPPAVVCPSSDSLYTT
                     GISCGATYIVPTISGTDNCAGMIVGGTVQVTVAGGGSVTVDGADTVADDPVDLGTGSE
                     VVLPPGVHLFVDTIFDGNGNFSVCNWSITVLDTVPPVLACPPGTVSINADAASCTGTM
                     IDLLGLVAIVGDNCTSDADLVLTQTPAIGAVVSSGDAAAVNLADSTGNSTTCTFSISV
                     IDAEAPVFDNCPSPSNMEVDPPLGACSYLYVFPTITSTDNCDPDTETGYRTFLLEAGN
                     SIWQEVTGQTDHALAPGIHQLYEVHADDAGNSDTCFWSVTVRDDEAPVITVPANILVS
                     AEANACGATVSFAVGVSDNCAVLDTTFSQASGSFFPSGTTTVTVDANDINGNAATQAS
                     FTVTVEDDTPPSITCPGNIARNISDPITCTHSAAWPQPTFSDNCSSASITQTSGLPSG
                     SQFSLGVHPISYTVQDADGNVASCAFTVTINDISAPSIVCPLYDTLPIPLGPGCSVAF
                     PDLRDSLTVTDCSPWTNAMDPLPGTVFTRDTVLYMTMWVEDSAGNGLSNDHWVHIMDL
                     EPPTINCPADINTNATAGTCGATVNYAAPVGMDNCSSPITTLSMGLASGSLFPIGSTT
                     VSYHVIDAAGLTAGCSFTVTVEPSVLPVLNYSQSNFCETSGMQSPLAAMPIGG"
BASE COUNT          535 a          831 c          963 g          573 t
ORIGIN      
        1 tcccccgatg ggcatcgcgg ccaatggtga ctgcataccg ctggtctcgc agaaattgga
       61 ttgagaatag ttcaatacgg gcagtacact gggctccacc gtcaccgtga aggagcaacc
      121 ggccgtgagg cccgcagcat cgatcacgtg gtagctgacc gtggtgctgc cgatggggaa
      181 gagggatccg ctggcgagtc ccatgctcag cgtcgttatc gggctcgagc agttgtccat
      241 tccgaccgga gcagcgtagt tcactgttgc accacaggtc ccagcggtag cattcgtgtt
      301 gatgtccgca gggcagttga tggtcggagg ctcaaggtcc atgatgtgga cccagtggtc
      361 gttcgacaag ccgttgccgg cgctgtcctc cacccacatg gtcatgtaca gcacggtatc
      421 tcgcgtgaaa acggtgcctg gcagcggatc catcgcgttg gtccaagggc tgcaatcggt
      481 cacggtcaac gaatcgcgca gatccgggaa tgccactgaa cagccgggtc ccaacgggat
      541 cggaagggtg tcgtagagcg ggcagacgat ggatggggcg gagatatcat tgatcgtcac
      601 cgtgaacgcg cacgaagcga cgttgccatc agcgtcctgt accgtgtagc tgatcggatg
      661 cacgcccaag gagaactggc ttccgctggg aagtccggag gtctgggtga tggatgcgct
      721 ggaacagttg tcactgaacg ttggctgggg ccatgctgcc gagtgtgtac aggtgatcgg
      781 atcgctgatg ttccgtgcga tgttcccggg gcaggtgatg ctcggcgggg tatcatcctc
      841 cacggtgacc gtgaacgaag cctgtgttgc agcattgccg ttgatatcgt tcgcgtccac
      901 ggtcaccgtg gtggtgccag aagggaagaa ggagccgctg gcctgactga aggtggtgtc
      961 cagcaccgcg caattgtcgc tcacccccac cgcgaagctc actgtggcac cgcacgcatt
     1021 ggcctccgcg ctcaccagga tgttcgcagg gaccgtgatc accggtgctt cgtcatcgcg
     1081 caccgtcaca ctccagaagc aagtgtcgct gttaccggca tcgtccgcat gtacctcgta
     1141 gagctggtgg atgccgggcg cgagagcgtg atccgtttgg ccggtcacct cttgccaaat
     1201 gctgttgccc gcttcgagca gaaaggtccg atatccggtt tccgtgtcgg ggtcgcaatt
     1261 gtccgtgctg gtgatcgtgg ggaagacgta caggtagctg catgcgccca gcggcggatc
     1321 gacctccatg ttcgagggcg acgggcagtt gtcgaacacc ggtgcttccg catcgatcac
     1381 ggagatggag aacgtgcagg tggtgctgtt ccccgtgctg tcggcgaggt tcacggcggc
     1441 cgcatcaccc gaactgacca cagcaccaat ggccggggtc tgggtgagca ccagatccgc
     1501 atcggaggtg caattgtccc ctacgatggc caccagtccc agcaggtcga tcatcgtgcc
     1561 ggtgcaggaa gcggcatccg cattgatgct cacggtgcct ggtgggcagg cgagcacggg
     1621 tggaaccgtg tccagcaccg tgatcgacca gttgcacacg gagaagttcc cgttgccatc
     1681 gaagatcgtg tcgacgaaga gatgtacacc gggaggcaga acgacctcgc ttcctgttcc
     1741 gaggtcaacg gggtcgtcgg ccactgtgtc ggcaccgtca accgtaacgc tgccgccgcc
     1801 agccaccgtg acctgtacgg tgcctccgac gatcatgccc gcgcaattgt ccgtcccgct
     1861 gatggtcggc acgatgtacg ttgcgccgca ggagatgcct gtcgtgtaga gcgagtcgct
     1921 ggatggacac accacggcgg gtggggtcgt gtcctcaacg accaatgtcg ccacggaact
     1981 tgtcgccatg ccgcacgctc cgctcttcag ttgggcacgg aactgcaagg tgccgccgat
     2041 gctgctccag ggcagtgtgg tggtcgtgtt cggtatattg gtccaaaggg tgccgtcggg
     2101 gctgctctgc cagcgttgca cgatgccggt atgtccgctc aagatcagta tcccggtact
     2161 gctcggacag tagatgcccc caccggagat actgcctcca acggcattcg gatccacctg
     2221 gatgatcttg ttggacgagt aggtacatgc gcctgaagcg accgtgtagc tcacggggta
     2281 gcttcccgga ccggtcggcg tgaagatccc tgtcgccgtc acgtgcggtc cgccgctcca
     2341 tgtgccgccg ctcaacgtgt tgttcaggta gcgcaatgca tccacttgca gggtggtgaa
     2401 gctgaccgag ccggctcgtt gaaggcggat gaaccgtgcg gtggagggaa cggtgtatat
     2461 caccgaattc cacgtagtgc tcgaagtgga ccaggtctgc ggtgcgccgt aggtgatctg
     2521 atccaggctt gaggagatga gcaactgcgc cggaaccccg ttcgtttggc tgcgccacca
     2581 tacgcgcacc gattcgccgg cctggatggt gtctcccagg tcgagcacga gttcggcacc
     2641 gggcgctgtg aagaccgccc cggaattgtc gggtgcgttg atggcattgc cagggtcgct
     2701 taccgaaagg ctgcttgcta cgccggaagc atagtcgacc aggaaggcgt tcaggtcgat
     2761 cggtgctgtg gcttcgcaga aaggccctgg ttgcgcgaag cgggagggat cattggcgaa
     2821 ggcgaggtag ttcggatcgg tgaatccggc cacgaacagg tcatcgccgg agccgttcgg
     2881 gatcacggtg gaacccagca cc
//
LOCUS       DAOQOC010000272         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18389_length_2901_cov_2.756852, whole genome shotgun sequence.
ACCESSION   DAOQOC010000272 DAOQOC010000000
VERSION     DAOQOC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18389_length_2901_cov_2.756852"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2748)
                     /locus_tag="PL106_05485"
     CDS             complement(<1..2748)
                     /locus_tag="PL106_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNA32554.1"
                     /translation="MKLTLRIPRPTRSLMGLAALALAATTWAQTNPTPQALPYTQDFS
                     SLAHSSTVYPAGWQGWAIGGNPGASYKTTGPTTDRVLEASGDAAGTSGNVYNYNGKIG
                     FLNSGSLDLTVGLALNTTGFSSVQVVYDIATIRNPHNGGANTRINEVSLQYRVGNSGG
                     WTTISGQEYQNNTTTQTNSGNTDPQNQQTKTVTLPNACDNQAEVQVRWASRQVSGSGS
                     RPSFSVDNLNVSGSGLDNTSVSFATGSSVVSEGAGSTNLTVTISSFSTTQATTVDVAI
                     GIGDPARVGNYTTQTVTFPANDGTPQNVPVTITDDGVYDGGATVIFILQNVTGGQGTP
                     TIGTPLDHALTITENEPSPLVINEVDYDNPGSAEDMEFVEILNNGLVAIDLNGYQIEL
                     VNGSGGAPYSTITLPSFSLAPGAYYVVGNNASIPNINLVTGAPSMIQNGSPDAVALRS
                     PSNDLLDVISYEGSVAAPYTEGTGLDVANSDDGTPGIGISRFPNGADINDNSVDWSRR
                     CLSPGASNNSTFEFCICEPPTATATPQCIDDNTWEIVVDVTDLGSASSVDITNNVNGA
                     SAIGVGLGQHIIGPFNNNAVVDVLVHPDDTSFCDVNLPGNFMNCMPDCLGVPGGWDVP
                     GAPCDDQDPNTVNDTWSPTCICEGAPPQPTVSFSLPFSAVAEDGVSLTIGLEMDMAPN
                     APVTVEVTDLFTGTATSGTDYAPIGTVQLTFLPEDFYPALMTFNVLITDDLDDEPAET
                     FDLALDVTNGTATVGNGNFTGQIQDNDVPALVINEVDYDNAGADNAEWIELKNTGAAT
                     IELENWTVELVNGNGGGAAVYKTITLPAFSLAAGGYYVIGNNASTPNINLVVTPASDL
                     IQNGAPDAIGLRSPDNSLVDAISYEGNTGSPYLEGVGSPVAQADDNTTAAKVIARYLD
                     GN"
BASE COUNT          512 a          846 c          900 g          643 t
ORIGIN      
        1 gttgccgtca aggtagcgcg cgatcacctt ggcggccgtg gtattgtcat ccgcttgggc
       61 tacaggcgat ccgacgcctt ccaggtacgg cgaaccggtg ttgccctcat aactgatcgc
      121 atccaccaag ctgttatcgg gggatcggag tccgatcgcg tcgggtgctc cattctggat
      181 caggtcacta gcaggtgtga ccaccaagtt gatgttgggg gtggaggcgt tgttcccgat
      241 cacgtaatac cctccagcag caagactgaa tgcgggcagg gtgatcgtct tataaacagc
      301 cgcacctccg ccgttgccat tcaccagctc gacggtccaa ttctcgagtt cgatcgtcgc
      361 agcaccggta ttcttcaatt cgatccattc ggcattgtcc gcgccggcgt tgtcataatc
      421 gacctcgttg atcacgagcg cgggtacatc gttgtcctgg atctgcccgg tgaagttccc
      481 gttgcccaca gtggccgtgc cgttggtaac atccagtgcg agatcgaagg tctcagcggg
      541 ttcatcgtcc aggtcgtcgg tgatgagcac gttgaaggtc atcaaggccg ggtagaaatc
      601 ctcgggcagg aaggtgagct ggacggtgcc gatgggggcg tagtccgtcc cactggtggc
      661 cgtgccggtg aagaggtcgg tcacttccac ggtcacaggt gcgttgggcg ccatgtccat
      721 ctccagaccg atggtgagcg ataccccgtc ctcggcgacc gcgctgaatg gcaggctgaa
      781 ggagaccgtt ggctgcgggg gagcgccctc gcaaatgcat gtgggactcc aggtatcatt
      841 caccgtgttc ggatcctgat cgtcgcatgg tgcacccggt acgtcccagc cacccggaac
      901 tcccaggcaa tcgggcatac agttcatgaa gttgcccggc aggttcacgt cgcagaagct
      961 cgtgtcgtcc ggatgaacca gcacatccac tactgcgtta ttgttgaagg gcccgatgat
     1021 gtgctggcca aggcccacgc cgatcgcgct ggcgccattc acattattgg tgatgtccac
     1081 cgagctggca ctgcccagat cggtcacatc caccacgatc tcccaggtgt tgtcatcaat
     1141 gcactgaggg gtggctgtag ccgtgggtgg ttcgcagatg cagaactcga aggtgctgtt
     1201 gttgctggca cccggactca ggcagcgtcg gctccaatcc acgctgttat cattaatgtc
     1261 cgcaccattc gggaagcggc tgatcccgat gcccggtgtg ccgtcgtcgc tgttggccac
     1321 atccagacct gtgccttcgg tgtacggcgc tgcaacggaa ccctcatagc tgatcacatc
     1381 cagcaggtcg ttggatgggc tgcgcaaggc gaccgcgtca ggggatccgt tctggatcat
     1441 gcttggcgcg ccggtcacca ggttgatgtt cggaatggag gcgttgttgc ccaccacgta
     1501 ataggccccg ggagccagag agaaactcgg caaggtgatg gtggagtacg gtgccccgcc
     1561 ggagccgttc accagctcga tctggtatcc gttcagatcg atcgcgacaa gaccattgtt
     1621 caggatctcc acgaattcca tgtcctcggc cgatccggga ttgtcatagt ccacctcgtt
     1681 gatcaccaac ggcgagggct cgttctcggt gatggtgagc gcgtggtcca atggagtgcc
     1741 gatggtcggt gtgccctgac cgccggtcac attctgtaag atgaagatca cggtggcccc
     1801 gccatcatac accccatcgt cggtgatcgt cacgggcacg ttctgcgggg tgccgtcatt
     1861 cgcagggaag gtcaccgttt gtgtggtgta gttgcccacg cgcgccggat cgccgatccc
     1921 gatcgccaca tccaccgtgg tggcttgtgt ggtgctgaaa ctgctgatgg taacggtgag
     1981 gttcgtgctg cccgcacctt cgctcaccac gctcgatccg gtagcgaagg acacgctggt
     2041 gttgtccagc cccgatccgg acacgttcag gttgtccacc gagaagctgg ggcgactgcc
     2101 acttccactc acctgacggc tggcccagcg cacctgcacc tccgcttggt tgtcgcaggc
     2161 gttgggcagg gtcacggtct tggtttgctg gttctgcgga tcggtgttgc ccgagttcgt
     2221 ttgggtggtg gtgttgttct ggtactcctg gccactgatc gtggtccacc caccggaatt
     2281 ccctacgcga tactgcaaac tcacctcatt gatccgtgtg ttcgcacccc cgttgtgcgg
     2341 gttgcggatc gtggcgatgt cgtacaccac ctgcacgctg gagaaccccg tagtgttcag
     2401 tgcaagcccg accgtcagat ccaatgaacc gctgttcagg aagccgatct taccgttgta
     2461 gttgtacaca ttgcccgagg tgcccgccgc gtcgccggac gcctccaaca cgcgatcggt
     2521 cgttggaccg gtggtcttgt aactcgctcc tggattgccc ccgatcgccc agccctgcca
     2581 accggccggg tacaccgtgg atgaatgtgc caagctgctg aaatcctgcg tgtagggcag
     2641 ggcctgcggc gttggattgg tctgggccca ggtggttgct gccaaggcca acgcggccag
     2701 acccatgagt gaacgggtcg gccgaggaat gcgtaaggtc agtttcatgt acgtaacgat
     2761 tggggtgacg ggttcgggga agccaaacta tcggatcggt gtgaattgtc ggaaaaaaat
     2821 attttccaca gcaattgcgc tcggtccata ggtcggttct gccctggcgg taccgttggc
     2881 ccagggcctt caggtgctgt t
//
LOCUS       DAOQOC010000273         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18435_length_2897_cov_3.358902, whole genome shotgun sequence.
ACCESSION   DAOQOC010000273 DAOQOC010000000
VERSION     DAOQOC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18435_length_2897_cov_3.358902"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(487..621)
                     /locus_tag="PL106_05490"
     CDS             complement(487..621)
                     /locus_tag="PL106_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32555.1"
                     /translation="MRLLSPTTDATRKGARPYVEDRLDAVEREVRGLRTELKNQQKKR
                     "
     gene            943..1173
                     /locus_tag="PL106_05495"
     CDS             943..1173
                     /locus_tag="PL106_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32556.1"
                     /translation="METRLAFRLRHLTAQQRVELCAQLNVSDRTLYRIADHPGSMLTL
                     EQADRLRQYLEAVDNCDYDMYQLLREVDITTA"
     gene            1177..1401
                     /locus_tag="PL106_05500"
     CDS             1177..1401
                     /locus_tag="PL106_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32557.1"
                     /translation="MSTPYIAHEAAAPDGTARYALHGLTQKDMECLETSLELTANLFT
                     NTPDIQDALTPEIVAHLKELMRILQGTRKL"
     gene            1405..1917
                     /locus_tag="PL106_05505"
     CDS             1405..1917
                     /locus_tag="PL106_05505"
                     /inference="COORDINATES: protein motif:HMM:NF026141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32558.1"
                     /translation="MDYKTALATANHLMERFAPHCERIEIAGSIRRKKAIVKDIELVC
                     IPKQEPTGLFDMDLRHSMGFAALCLELSRGGWKKGDTGSGKFIAINTATGLRIDIFTT
                     TADNWGYILTLRTGSSDHNIRLMQRLKSNGYRCADGHVMWKGEAREMVDEMAVFDAAG
                     LKWVDPQFRV"
     gene            1924..2409
                     /locus_tag="PL106_05510"
     CDS             1924..2409
                     /locus_tag="PL106_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32559.1"
                     /translation="MTAEQAISSIREDIAALDRKNLVKPGHIRRRTAEVDAIVHALAD
                     YEAHQFRLEARIADIEREGERDRLILGMLANMLTILGFPWQSRARYLNADEAAIMSRA
                     AAIAAEQNINISDANTPKWRLRETLQLALIAARMERRLNAIPTPFTAHAQATEEEAKH
                     Q"
     gene            2372..>2897
                     /locus_tag="PL106_05515"
     CDS             2372..>2897
                     /locus_tag="PL106_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32560.1"
                     /translation="MPKQQKKKPSTNRKGTKPVKVKDLAKVEEEGTYKLPKGCELERV
                     ERLIKRYKCFEFERQVWSMRESGWAVPVSNFTCDIHMHIIDEVPTRLISVENVDKEEY
                     VLHVPHDVFNSLPAFRKAVTGRGNYQWTGSEVDYMRYLSMMMDRMGTGRMILEPGMQR
                     EGFFCFVNRVCHTDT"
BASE COUNT          659 a          884 c          825 g          529 t
ORIGIN      
        1 atgttcggca cgtgccatcg ccaaggcttc ctggccgttg gatgccgtgc gaactaggta
       61 gccttcgcgt tccaggttgt acttcagcag atccaggata tcgcgttcgt cgtccaccag
      121 caggatcttt tgcggtgacg gttcagaggc gagctgcttt ttttccggaa ccaaggtgga
      181 tatcggtctt gttgtcgggg gcacgccccg gtcagatcaa gcgccgcaaa ggttacggtt
      241 tcagggcagg atcgggtcgg agttatcagg gttgttttcg aaccgacgct tggaccgagg
      301 agttgacaac atcttcaggc cagtcctgag ttccgtagcc tgatcctgcg cataatttat
      361 actttagcag gtatgcgccc cctgcgcctc agcaggtttg ccttcaaccg cgcgatcgat
      421 cccgccaagc ccgagcgatc ccccaaggcc gatcgttcga ccaaacgata gggcaggtat
      481 aatttctcat cgcttcttct gttggttctt cagttccgtt cgcaagccgc gcacctcccg
      541 ttccacggca tccagtctat cctcaacata ggggcgtgcc cccttgcgtg tggcgtcagt
      601 ggtgggggag agtaggcgca tggggtccac gctaaggatg gcagaaatct gagccaacct
      661 ctgcaagtcc atagtgccat tcttcagcat gcgccaataa ccactttccg acatgtccag
      721 agccctccat acatcggtgt ggctcttgcc cagctccacc aacacctgtt gaaggcgatc
      781 cgcgaccacg ccagtagcgg tttcaagagc actttgatag gcgctgtcca cggacgtgtg
      841 tgcaaaagca cttacctcgt gtgtcagtat ccctgacccg agggtatagc cttgcagtgt
      901 caatatagac actcaccgcc aatccagaca ctctgcgcca ccatggaaac ccgccttgcc
      961 ttccgccttc gacatctcac cgcgcagcag cgtgtggaac tgtgcgccca gctcaacgtg
     1021 agcgaccgca ccctgtatcg catcgccgat caccccggaa gcatgctcac cctggagcag
     1081 gccgatcggc tgcgccaata cctggaggcc gtggacaact gcgactacga catgtaccag
     1141 ctcctccgag aggtggacat caccaccgcc tgaaccatga gcacccccta catcgcccac
     1201 gaagcagccg ctcccgatgg aacggcgcgg tacgccctgc atgggctcac ccagaaggac
     1261 atggaatgcc tggaaacgag cctggaactc accgcgaacc tcttcaccaa caccccggac
     1321 atccaagatg ccctcacccc cgagatcgtg gcgcacctga aggaactcat gcgcatcctt
     1381 caaggtaccc gaaagctctg agcgatggac tacaaaaccg cactggccac ggccaaccac
     1441 ctcatggaac gcttcgcccc gcattgcgag cgaatcgaga tcgccggaag tattcggagg
     1501 aagaaggcca tcgtgaagga cattgaactc gtgtgcattc ccaaacagga accaaccggt
     1561 ctgttcgata tggacctgcg gcattcgatg ggcttcgctg cactgtgcct ggagctttcc
     1621 cgtggcggtt ggaagaaagg agatacaggc agcggcaagt tcatcgccat caacacagcc
     1681 acagggctcc ggatcgacat cttcacgaca acggcagaca actggggata catcctcacc
     1741 ctgcgcacgg gcagctcaga ccacaacatt cggctgatgc aacggctgaa gagcaacgga
     1801 taccgctgtg cggatgggca tgttatgtgg aagggggagg ctcgtgaaat ggtggatgaa
     1861 atggcggtat tcgatgcagc ggggctcaag tgggtggatc ctcaattccg agtgtgaggc
     1921 tccatgaccg ccgaacaggc catctccagc atacgcgagg acatagctgc gctcgatcgg
     1981 aagaacctgg tgaagcccgg gcacatccgt cgccgcacgg ccgaggtgga cgccattgtt
     2041 cacgcactgg ccgactatga agctcaccag ttcagattgg aggcccgcat cgccgacatc
     2101 gaacgagagg gggaacgcga ccgcctcatt ctgggcatgc tggccaacat gctcaccatc
     2161 ctcggtttcc cgtggcagag cagagcgcga tacctgaacg ccgacgaggc cgccatcatg
     2221 tcccgagcgg ctgccatcgc agcagaacag aacatcaaca tcagcgacgc caacacgccg
     2281 aaatggaggc tgcgtgagac cctccagctc gcactgatcg cggcgcgcat ggaacgacgg
     2341 ctgaacgcca ttccaacccc tttcacagca catgcccaag caacagaaga agaagccaag
     2401 caccaatagg aagggaacca agccggtgaa ggtgaaggac ctggccaagg tggaggagga
     2461 gggcacctac aagctgccga aaggctgcga actggagcgg gtcgaacggc tcatcaagcg
     2521 ctacaagtgc ttcgagttcg agcggcaagt gtggtccatg cgcgaaagtg ggtgggccgt
     2581 gcccgtcagc aacttcacct gcgatatcca catgcacatc atcgacgagg tgcccacgcg
     2641 gctcatatcg gtggagaacg tggacaagga agagtatgtg ctccacgtgc cccacgacgt
     2701 gttcaacagc ttgccagcgt tccgaaaggc cgtgaccgga cgcggcaact accagtggac
     2761 agggagcgag gtggactaca tgcgctacct gtccatgatg atggaccgca tgggaaccgg
     2821 gcgcatgatc ctggagccag gcatgcaacg ggaaggcttc ttctgcttcg tgaatcgcgt
     2881 gtgccacacc gacacag
//
LOCUS       DAOQOC010000274         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18453_length_2896_cov_2.831045, whole genome shotgun sequence.
ACCESSION   DAOQOC010000274 DAOQOC010000000
VERSION     DAOQOC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18453_length_2896_cov_2.831045"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PL106_05520"
     CDS             <1..241
                     /locus_tag="PL106_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HNA32561.1"
                     /translation="LPDEVSAEVRERSAAIYRALGCRGLVRVDHFWTGSEVVTIEVNT
                     TPGFSPASIFPKMLKVSGIGVPRAINGLVEEMLQR"
     gene            complement(232..816)
                     /gene="rdgB"
                     /locus_tag="PL106_05525"
     CDS             complement(232..816)
                     /gene="rdgB"
                     /locus_tag="PL106_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNA32562.1"
                     /translation="MARMVLCTGNPGKIAELRALMPPDMELLSLADVGLPADLPETGA
                     TLEANALQKARFAHERTGLPCIADDTGLEVLALGGAPGVRSARYAGEAKDPAANMALL
                     LRELATATDRSARFRTVIALVDARGEHVFEGQVRGRITTDRRGSGGFGYDPVFLPEMS
                     DLTFAELDPVRKNAISHRGQAVWKLARFLAERQR"
     gene            complement(824..1651)
                     /locus_tag="PL106_05530"
     CDS             complement(824..1651)
                     /locus_tag="PL106_05530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA32563.1"
                     /translation="MMPKFTNRQVRVLVLVPLAVLAVSAAAYLALGSPALLLPPVLMG
                     AFVVWMLMDMRHHHWDLFHRDVMEQRAIFAQLEAIIGLNATLKPKYPFPGTRGWTASP
                     DLLREVVTHVLHQAPALTVEASSGTSTLMIAYALERNGAGHVVALEHDAEYAGRTREL
                     IGMHGLDHRATIVLAPLVKQRVGNAEMTWYDLGEVRFDVPIDLLVVDGPPEGTGAMAR
                     YPAVPLLRSRFAPGARVILDDGDRADERAIAERWEQEFKASSCDHLALEKGAWSLRF"
     gene            1757..>2896
                     /locus_tag="PL106_05535"
     CDS             1757..>2896
                     /locus_tag="PL106_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32564.1"
                     /translation="MHLRGKNILIISNEAWGDVWFSKHNYAYELSRNNTVVFVDPPGP
                     WRPKNLLGMAPSLHKVGSSLTVLRYRNRLPVLNKWFYRLNERLVARGLRAFLRRNGLA
                     PGTLITFDPTRITAPQRLRPERSLFICVDKYNFTFHGERELCHNVDAIVTISEHLSVR
                     YERFNKPLLTIGHAISSDEFEAEPAPDVPEGYGLYVGTIDARVDHAQVARMLERFPQV
                     PFLFVGRLALKEDDPYRSIYLPGRYANMHHVGVRPFKELKSYIAGSRFCLAPMDLRFP
                     GNDISHHKTFQYLALGKPVFSTVFSEYLPIAHLMYMENDPVRGLDQLERFLEEGEAVG
                     LTAERVAHARAMTYERTFERIERFLNEVRSADTAHLTIFLFSNEPW"
BASE COUNT          531 a          967 c          894 g          504 t
ORIGIN      
        1 ccttcccgac gaggtgtcgg ccgaggtgcg cgaacgcagc gcggcgatct accgggcgct
       61 cggctgccgg ggcctggtgc gcgtggacca cttctggacc ggcagcgaag tggtgacgat
      121 cgaggtgaac accacgcccg gtttcagtcc ggcgagcatc ttccccaaga tgctgaaggt
      181 gtcggggatc ggcgtgccgc gggcgatcaa cggcctggtg gaggagatgc ttcagcgttg
      241 acgttcggcc aggaagcggg ccagcttcca cacggcctgc cccctgtggc tgatcgcgtt
      301 cttgcgcacg gggtccagtt cggcgaaggt cagatcactc atttccggca ggaaaacggg
      361 atcgtacccg aagccgccgg agccacgccg atcggtggtg atgcggccac ggacctgtcc
      421 ttcgaaaacg tgttcacccc gggcatccac cagggcgatc acggtgcgga aacgcgccga
      481 acggtccgtt gcggtggcca gttcgcgcag caatagggcc atgttggcgg cggggtcctt
      541 cgcctcaccc gcatagcggg cactacgtac cccgggggca ccgcccaatg ccaatacttc
      601 caaaccagta tcatcggcga tgcagggcag gccggtgcgc tcgtgggcga agcgggcctt
      661 ttgcagggcg ttggcttcca gcgtggcccc tgtctcaggc aggtccgcgg gcaggccgac
      721 atccgccagg gagagcagtt ccatatccgg tggcatgagc gcgcgcagtt cagcgatctt
      781 tccggggttg cccgtgcaga ggaccatgcg tgccatgtgc ttctcagaac cggagcgacc
      841 acgcgccctt ttccaacgcg aggtggtcgc acgaggaggc cttgaactcc tgctcccatc
      901 gttcagcgat cgcgcgctca tccgcccgat caccgtcgtc caggatcacg cgggcccccg
      961 gtgcgaagcg ggaacgcaac agcggcacgg cgggatagcg ggccattgct cccgttcctt
     1021 ccggagggcc gtcgacgacc agcaggtcta tgggcacatc gaaccggacc tcgcccagat
     1081 cataccacgt catctccgca ttcccaacac gttgcttcac caacggggcc agcacgatgg
     1141 tggcgcggtg gtccagcccg tgcatgccga tcagctcccg cgtacgcccg gcgtattccg
     1201 catcatgctc caatgccacg acatgtccgg caccattgcg ctccaaggca taggcgatca
     1261 tcagggtgga ggtgccgctg ctggcttcca cggtgagcgc cggggcctgg tgcagcacgt
     1321 gtgtcaccac ttcacgcaac aggtcgggtg aggccgtcca gccacgtgtg ccggggaagg
     1381 gatacttggg cttgagcgtc gcgttcaacc cgatgatggc ctccaactgc gcgaagatgg
     1441 cccgctgctc catcacatcc cgatggaaaa gatcccagtg atggtgccgc atatccatca
     1501 acatccagac cacgaaggcg cccatgagca ccggcgggag cagaagcgcc ggggatccca
     1561 atgccaggta tgcggccgca ctcacggcca gcaccgcgag cggcaccagg accagcaccc
     1621 gtacctgtct gttggtgaac ttcggcatca tgagcgtccg ggcgcaagaa taggtgcgaa
     1681 aacgatgatc ccggtcctgc cgcgcctgcg acacgtccgg tacattcgcc cgccctaccg
     1741 acccggccga accggcatgc acctgcgtgg caagaacatc ctgatcatct ccaatgaggc
     1801 atggggtgat gtgtggttct ccaagcacaa ctacgcgtac gaactgagcc ggaacaacac
     1861 cgtggtgttc gtggacccgc ccggcccatg gaggccgaag aacctgctgg gcatggcccc
     1921 gtccttgcac aaggtaggca gctcgctcac ggtgctgcgg taccgcaacc ggctgccggt
     1981 gctgaacaag tggttctatc gcctgaatga acgtctggtg gcgcgcggcc tccgcgcgtt
     2041 cctgcgccgg aacggactgg ccccggggac cctgatcacc ttcgacccca cacggatcac
     2101 cgctccacag cggttgcgcc cggagcgctc cctgttcatc tgcgtggaca agtataattt
     2161 cacgttccac ggggagcgcg agctgtgcca taatgtggac gcgatcgtca cgatctccga
     2221 acacctttcc gtccgctacg agcggttcaa caagcccctg ctcaccatcg gccacgccat
     2281 ctcgagcgat gaattcgagg cggagccggc accggatgtc cccgaaggct acggtctgta
     2341 cgtgggcacg atcgatgcac gggtggacca cgcgcaggtg gcccgcatgc tcgagcgatt
     2401 cccgcaggtc ccctttcttt tcgtcggtcg gctcgcgctg aaggaggatg acccctacag
     2461 gtccatctac ctccctggtc gctacgcgaa catgcatcat gttggtgtga ggccgttcaa
     2521 ggaactcaag tcctacatcg ctggcagccg attctgcctg gccccgatgg acctgcgatt
     2581 cccggggaat gacatctccc accacaagac cttccagtac ctcgctctgg gcaagccggt
     2641 cttctccacg gtcttttccg agtacctgcc catcgcgcac ctgatgtaca tggagaacga
     2701 tccggtccgg ggcctcgacc agctcgagcg attcctggag gaaggggaag ccgtcggact
     2761 caccgcggaa cgcgtggcac acgcgcgcgc catgacctac gagcgcacct tcgaacgcat
     2821 cgagcggttc ctcaacgagg tacgatcagc cgacaccgcg catctcacca tcttcctgtt
     2881 ctccaatgaa ccctgg
//
LOCUS       DAOQOC010000275         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18514_length_2890_cov_3.089947, whole genome shotgun sequence.
ACCESSION   DAOQOC010000275 DAOQOC010000000
VERSION     DAOQOC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18514_length_2890_cov_3.089947"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PL106_05540"
     CDS             complement(<1..497)
                     /locus_tag="PL106_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNA32565.1"
                     /translation="MNIPPSNIFTLFQQTATRIPQQAALINGQSTFSFAELGARAGRL
                     AALLQKTGAERGATVALCLDRSPELIIAVLGSILINSEALFEIPYLQPEDFYLVKHRW
                     IWNAFIALNESRQPLDVLTVARELERHNQLDEAGGQGYLTQLVTAVPTSIHAEGYARI
                     VQRDAT"
     gene            1047..>2890
                     /locus_tag="PL106_05545"
     CDS             1047..>2890
                     /locus_tag="PL106_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32566.1"
                     /translation="MQRSTLALLGLVCTLSLSAQTLSQKASVLVSATVSASPATITLN
                     WSSFSGSTGFTISRKLKSATSWTQIGTAGGGATQFVDNNVTVGEYYEYKVDRSASAGS
                     GVGYIASGIEVPVVDFRGKMVLLVDNTFTSSLATQLTQLVADLRADGWVVLRHDVDRN
                     AAPPSIRSIIQNDYNADPTNVKAVFLFGHIPVPYSGNINPDGHGDHQGAWPADGYYGE
                     MNGNWTDNSVNNTSANSLRNRNTPGDGKFDQSDYPSTVELQVGRVDLYNMESFQFYAG
                     LNEQALLSNYLTKLHNFKTKQFTPQFRGCVFDHFSDPVMGPTASSGYRSVSANVGPAN
                     MQDLNGSGAPFSTYVNNQSWLWTYACGGGGWTLASNVCSTGEYAQNVVMGGVFNICIG
                     SYFGDWDNANNFLRAPLGSGKALTNAWVGWPNWWFHHMGMGDNIGYSTVTSMNDPSSV
                     YWPQNGGWHGNSLYRVHMGLMGDPSLRQMMVNMPSNLQVTNSGGLANFSWTAASGNPL
                     GYHIYDMGPSASSIPVRITSSPVTGTSFSSPTVPFVAGRQYMVRAVKLEVGASGSYKN
                     MSLGTFGTASGQAAPDCLGNVGGPAVPGSSCNDNNACTINDVYNSSCQ"
BASE COUNT          563 a          819 c          908 g          600 t
ORIGIN      
        1 gtggcgtcgc gctggacgat acgcgcgtag ccttcggcgt ggatcgaggt cgggacggcc
       61 gtgacgagtt gggtgagata gccctggccg ccggcttcgt cgagctggtt gtggcgctcg
      121 agctcgcgcg ccacggtgag gacgtcgagc ggctggcgcg actcgttgag cgcgatgaag
      181 gcgttccaga tccagcgatg cttgacgagg tagaagtcct cgggctgcag gtacgggatc
      241 tcgaacaggg cttcgctgtt gatcaggatc gagccgagca cggcgatgat gagctcgggg
      301 gagcggtcga ggcagagggc gacggtggca cctcgctcgg caccggtttt ttgcagaagt
      361 gcagcgagcc gtcccgccct ggcgcccagt tccgcaaagg agaaggtgct ttgaccgttg
      421 atgagggccg cctgctgcgg gatgcgagta gcggtttgct ggaagagcgt gaaaatgttg
      481 gacggaggga tgttcatcag cgttatgctc ggtcaggctg aattggacaa gtggcagtag
      541 atgccgcgat cgggtttgga cgtgtgaaac gactggtacg taaaatgcct ccagcaggtt
      601 aggctggtac gacgggttga aggtactctc gtgatcgacc gacggatagg aacggacgat
      661 gaagttctcc tgaccggtgc atccgcgaat gtccgatcgc ggtttcaacc tgcgcatgag
      721 cgtggtcaac cggatacgat ggtgatcgcg ttgatcgtat gggtcgcacg ggtcaatggt
      781 gcttgtatcg ttcggcgcgg tgttggtcca gtgatctgtt gataactttc gtgtgtgttc
      841 attggtgttc gaccgggggg cacattcgtc gggccagatg ggattctcgc agaattttcg
      901 gtatgcgctg caaccccgat ggatactctt gcggtacaaa ggccgctttc ccatgcgtga
      961 acaccgcgat cacctgccag tagttgctgt gagcgacgat tccgccgcgc gcatgagggg
     1021 ggcacatctt ccaaccgcct gccatcatgc aacgatccac gctggccctt ctgggcctgg
     1081 tctgcacgct cagccttagc gcacagacgc tttctcaaaa agcttcggtc ctggtttcgg
     1141 ccacggtgtc ggcttcgccg gccaccatta ccctgaactg gtcgtcgttc tccggctcca
     1201 ccggattcac catcagccgc aagctcaagt cagctacgtc ctggacccag atcggcacgg
     1261 ccggtggtgg tgccacacag ttcgttgaca acaacgtgac cgtgggcgag tactacgagt
     1321 acaaggtcga tcgctccgcc agcgcagggt cgggcgtcgg ttacatcgcc tcaggcatcg
     1381 aggtgcccgt ggttgatttc cgagggaaaa tggtgctctt ggtggacaac accttcacct
     1441 cttcgctggc cacccaactc acgcagttgg tcgcggacct gcgcgctgat ggctgggtcg
     1501 tcctgaggca tgatgtggac cgcaatgcag cgcctccgag catccgttcc atcatccaga
     1561 atgactacaa cgcggacccc accaacgtga aggccgtgtt ccttttcggg catattccgg
     1621 taccctattc agggaatatc aatccggatg gacatggcga tcaccaggga gcctggccgg
     1681 cggatggcta ctatggtgag atgaacggga actggaccga caacagcgtc aacaacacca
     1741 gtgccaacag tcttcgaaac aggaacacgc cgggcgacgg caagttcgac caaagcgatt
     1801 atccgagcac ggttgaattg caggtgggtc gggtggacct ctacaacatg gagtccttcc
     1861 agttctatgc cgggctcaac gagcaagcgc ttctgtccaa ctacctcacc aagctccaca
     1921 atttcaagac caagcagttc acaccgcaat tccgcggttg tgtattcgat catttttcag
     1981 acccggtcat ggggccgacc gcctcttcgg gctaccgcag cgtgtcggcg aatgtcggcc
     2041 cggccaacat gcaggatctc aatggaagtg gggctccctt cagtacgtat gtgaacaacc
     2101 agagctggtt atggacttac gcctgcggag gcggtggatg gaccttggcc agtaatgtgt
     2161 gctccaccgg cgaatacgcg cagaacgtgg tgatgggtgg ggtgttcaac atctgcatcg
     2221 gcagctattt cggggattgg gacaatgcga acaacttcct gcgtgcgccc ttgggaagtg
     2281 gtaaagccct caccaacgcg tgggtcggat ggcccaactg gtggttccac cacatgggca
     2341 tgggcgacaa catcggctac agcacggtga cgagcatgaa cgacccctca tccgtgtact
     2401 ggccacagaa tggcggttgg cacggaaact cgctctaccg ggtccacatg ggcctgatgg
     2461 gtgatcccag cttgcggcag atgatggtga acatgccctc gaacctgcag gtgaccaatt
     2521 cgggtgggct ggccaatttc tcttggacgg ccgcttcggg caaccccttg ggctaccaca
     2581 tctacgatat gggaccctcc gcttcgagca tccctgtccg catcacttcc agccctgtga
     2641 ccgggaccag cttcagcagc cccaccgtgc cattcgttgc gggccggcag tacatggttc
     2701 gtgcggtgaa actcgaggtc ggtgcctcgg gcagctataa gaacatgtcg ctcggcacct
     2761 tcgggaccgc atcgggccag gcggctcctg attgcctggg caacgtgggc ggccctgccg
     2821 tacctggttc gtcgtgcaac gacaacaacg cgtgcaccat caacgatgtg tacaattcca
     2881 gctgccagtg
//
LOCUS       DAOQOC010000276         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18555_length_2887_cov_2.581215, whole genome shotgun sequence.
ACCESSION   DAOQOC010000276 DAOQOC010000000
VERSION     DAOQOC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18555_length_2887_cov_2.581215"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..919)
                     /locus_tag="PL106_05550"
     CDS             complement(122..919)
                     /locus_tag="PL106_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNA32567.1"
                     /translation="MNYHLPHIPPRSPKPRESGLTMVMDKGLCLRQVEDLIAASGHLT
                     DLVKLGFGTSYVTPHLKEKIKLYRSAGIRVYLGGTLFEAFIARGLFKQYRKLLSDLGL
                     DCAEVSDGSINLPHEEKCKFINTLAQDHTVLSEVGSKETGILISPAKWVRMMNTELEA
                     GSWKVIAEARESGTVGIYRPSGHAHTALVNRIVAKVPKDDIIWEAPLKAQQTWFIKEL
                     GPNVNLGNVPAEEVIPLETLRLGLRGDTFFQHLPEEMVARLKQVNDE"
     gene            complement(916..2325)
                     /locus_tag="PL106_05555"
     CDS             complement(916..2325)
                     /locus_tag="PL106_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32568.1"
                     /translation="MHNSRRGHDRHEEQSDCVRRYESQLGREQPGFFDVSEFEMIIDH
                     YLANNEARRAQGALEMARRQHPGSVEVTFCEGLVHMGMGRLNKALEIFDALERIEPHT
                     PEIQLNKASIYSQQRNFRRAVEHYRRALQLAEEGLDEIYLDLAFEHENLEEYEVALDC
                     LKKAVELNPENETVLHELAFCFDLANADEASISWFRSFTNDNPYSAVGWFCLGNTLAK
                     LERFEESNEALDLALAIDERFTSAYFSRARNLLLAGHYAEAISLYEETLGFDGPQAIT
                     FSYIGECYEKMEHYEQALIHYDQAIHMDPTWADAWIGRGVVKDILNRLPEAIRDLENA
                     TRIAPESPDAWYYYANSLGRAKRWEDALDGYARLNSIEPQHLEGWMDHADMLLKLKGP
                     EAALSKFQECELVHKLNARYKYRLTSYLLRCGREQQALLELEEALMSDHSCHNTLLEH
                     YPEVANMPQVTHLLDLYGK"
     gene            2550..>2887
                     /locus_tag="PL106_05560"
     CDS             2550..>2887
                     /locus_tag="PL106_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNA32569.1"
                     /translation="MTLIKSISGIRGTIGGVPGDNLTPIDVVRFVSAFGTLIKQRSGR
                     TRPVCVVGWDARLSGPMVGDLVRATLTGLGFDVIVLGLATTPTVEMAVPGEGADAGII
                     LTASHNPAQW"
BASE COUNT          496 a          893 c          877 g          621 t
ORIGIN      
        1 caattcatga ggagtgatct ctttcatgtt caacaatggt caattcagaa acgggagaag
       61 tgcgacaagg ccggctggca gggggatcct tcgtcgctcc ccgctcccca ccccgcacgc
      121 ctcactcatc attcacctgc ttcaaccggg ccaccatctc ctcgggaagg tgctggaaga
      181 acgtatctcc gcgcagcccg agcctcaatg tttccagcgg gatcacctct tcggcgggca
      241 cgttgccgag gttcacgttg gggcccagtt ccttgatgaa ccaggtttgc tgcgccttca
      301 aaggtgcctc ccagatgatg tcgtccttgg ggaccttggc cacgatgcgg ttcacgagcg
      361 cggtatgggc atggccactg gggcggtaga tgcccacggt gccgctttcg cgtgcctcgg
      421 cgatcacctt ccaactgccc gcttcaagtt cggtgttcat catgcgcacc cacttcgccg
      481 ggctgatcag gatgccggtc tccttgctgc ccacctcact cagtacggta tggtcctggg
      541 cgagggtgtt gatgaacttg cacttctcct cgtgcggcag gttgatcgac ccgtcgctca
      601 cctcggcgca atcgagcccc aggtcgctca gaagcttcct gtactgcttg aaaagtccgc
      661 gcgcgatgaa ggcttcgaac agcgtgccac cgaggtacac acggatcccc gcgctccggt
      721 acaacttgat cttctctttc aggtgggggg tgacgtaact ggtgccgaaa ccgagcttca
      781 ccaggtcggt caaatggccg ctggccgcga tcaggtcctc cacctgtcgc aggcagagtc
      841 ccttgtccat caccatggtc agtcccgatt cgcgcggctt cgggctgcgc ggcgggatgt
      901 gggggaggtg gtagttcact tgccgtacag gtccagcaga tgtgtcacct gtggcatgtt
      961 cgccacctcg gggtagtgtt cgagcagtgt gttgtgacat gagtggtcgg acatgagtgc
     1021 ctcctccagt tccagcaagg cctgctgctc gcggccgcag cgcagcaggt aactggtcag
     1081 gcggtacttg tatcgggcgt tcagtttgtg taccagctcg cactcctgga atttcgagag
     1141 cgcggcttcc gggcctttca gcttgagcag catgtccgca tggtccatcc agccttcgag
     1201 gtgctggggc tcgatggagt tcaggcgtgc gtacccatcc agcgcgtcct cccaacgttt
     1261 ggcgcgcccg aggctgttgg cgtaataata ccatgcgtcc gggctttcgg gcgcgatacg
     1321 cgtggcgttc tccaggtcgc ggatggcctc aggcaggcgg ttgaggatat ccttcaccac
     1381 gccacggccg atccacgcat ccgcccaggt ggggtccatg tggatggcct ggtcgtaatg
     1441 gatcagggcc tgctcgtagt gctccatctt ctcgtagcac tcgccgatgt agctgaacgt
     1501 gatagcctgc ggaccgtcga agcccaacgt ttcctcgtac agggagatcg cctcggcgta
     1561 gtgtcctgcc agcaacaggt tgcgcgcccg gctgaaatag gcactggtga agcgctcatc
     1621 aatggccagg gccaggtcca gcgcctcatt gctctcctcg aagcgctcca gtttggccaa
     1681 ggtgttgccc aggcagaacc agcccaccgc gctgtacgga ttgtcgttgg tgaagctccg
     1741 gaaccagctg atgctggcct catcggcatt ggccaggtcg aagcagaagg ccagctcatg
     1801 tagtacggtc tcgttctccg gattcagttc cacggccttc ttcaggcaat ccagagcgac
     1861 ctcgtactcc tccaggttct catgctcgaa ggccagatcg aggtagatct cgtcaagccc
     1921 ttcctcggcc aattggagcg cacggcggta gtgctccacc gcgcggcgaa agttccgttg
     1981 ctggctgtag atgctcgctt tgttcagctg gatctccggt gtgtgcggct cgatccgttc
     2041 cagtgcatcg aagatctcga gggccttgtt cagccgcccc atgcccatat ggacgaggcc
     2101 ttcgcagaag gtgacctcca ccgaacccgg gtgctggcgc ctggccatct ccagtgcgcc
     2161 ctgggcccga cgcgcctcgt tgttcgcgag gtaatggtcg atgatcatct cgaactcgga
     2221 cacatcaaaa aatccgggct gctcgcggcc aagctgcgat tcgtaacgtc tgacgcagtc
     2281 gctctgctcc tcgtggcgat cgtgtcctct tcggctgttg tgcatccggt gccgatgcgg
     2341 cccgtcaagg ggtcagggag gcatcgcgtt gtgcgaaagt acacaagcga tcccggaaca
     2401 ttccgttcac cggtcaagca ggggatcaac aggggatcgt tcgccaagag aagcccctgt
     2461 ggatcaagag accaggtaat tttgcggccc cttttcgccg gaccgcgatc aatggctggc
     2521 tcgttcccac ggcgcaaggg cgctccgcta tgaccctcat caaaagcatc tccggtatcc
     2581 gtggcaccat tggtggtgta cctggcgaca acctcacccc gatcgacgtg gtgcgtttcg
     2641 tatccgcgtt cggcacattg atcaaacagc gatcgggtcg gaccaggccg gtatgcgtgg
     2701 ttggttggga tgcgcgcctg agtggcccca tggtcggcga tctggtgcgg gccacgctca
     2761 ccggactggg cttcgatgtg atcgtcctgg gtctggccac cacgcccacg gtggaaatgg
     2821 ccgtgcctgg cgaaggggcc gacgcgggca tcattcttac cgccagccac aaccccgcgc
     2881 aatggaa
//
LOCUS       DAOQOC010000277         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18560_length_2886_cov_4.241611, whole genome shotgun sequence.
ACCESSION   DAOQOC010000277 DAOQOC010000000
VERSION     DAOQOC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18560_length_2886_cov_4.241611"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1492)
                     /locus_tag="PL106_05565"
     CDS             complement(20..1492)
                     /locus_tag="PL106_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA32570.1"
                     /translation="MRILIALVAWCALCPHAHAAPVRIELTAAVHAGERALLYRYLDL
                     FTLRTELIGQAEVDADGKAVIEANVTGTVKAQLRIGGIHGGLWLRPGTYRITFPPPPP
                     GTPRSLNGTTETILEFRDLDRMDVNALLADLNQRLDDFAMEEVATSRRLGKDTAGRPE
                     TLFLSPTKSPARTDSFALKLQGYYNGVRDPWFLQNLEYGIAGLRFGPGVNDRELFGKY
                     LKGRPVLYDVPEYVRFIQAFFEEHIMRYPFRKNEAALVNAVRAADPDSVRTLFAEHDL
                     LKDDALCELVMLMGLHAQYPGKTFDRKGILGILDRVAATSMHPENRRIAANMLWDLTT
                     MRPGGRLPPLVLRDLSGQQARIDTVLRGATCLVVTAAWCTYCEQELVALETLRKEYGQ
                     VINVVGISLDRSPQELEAYVHAHPDRDWPWYFGGDNAVIMDQLRIRTIPAFFLLNGDT
                     LARAPAPPPSDGLAPILHKLKVQWEEENKLRPDQGPPRRR"
     gene            complement(1527..1985)
                     /gene="coaD"
                     /locus_tag="PL106_05570"
     CDS             complement(1527..1985)
                     /gene="coaD"
                     /locus_tag="PL106_05570"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNA32571.1"
                     /translation="MSERIAVFPGSFDPVTIGHVSIVERALPLFDRIVLAIGVNTTKK
                     YMFPVEQRLGWLRATFVGHKQVEVMAFEGLTADLCKRMKARYIVRGLRNSTDHGSERS
                     IALMNHVLAGVDTIFLPALPEHAHISSTIVRELIANKADVSAFVPGAVRP"
     gene            complement(1982..2557)
                     /gene="rsmD"
                     /locus_tag="PL106_05575"
     CDS             complement(1982..2557)
                     /gene="rsmD"
                     /locus_tag="PL106_05575"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNA32572.1"
                     /translation="MRIVGGKYKGRRIHPPRSTLARPTTDYAKEGLFNILQHTVPLEG
                     IRVLDLFAGTGSISLEFLSRKAGEVISVEQDRELFSHLQGAARALQATNWHHVKADAF
                     NFVKGHHATYDIIFADPPFNLEGTEQLPVLIREAGLLDPEGLLIIEHPKNVNMSTDPW
                     FDRHRPYGNVHFSFFKPSPAAQAGVINAERQ"
     gene            complement(2545..>2886)
                     /locus_tag="PL106_05580"
     CDS             complement(2545..>2886)
                     /locus_tag="PL106_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNA32573.1"
                     /translation="DKTAALIHRTAERLDLAIARGQQLLLSNTFHAATPEDVLYYVLY
                     ALEQCGTRPDDVRGLIAGTHLTGTEEDLLKKYLQDIGPAITPSDPNLAGLDLDRPHRF
                     TALLEQQACAS"
BASE COUNT          475 a          922 c          909 g          580 t
ORIGIN      
        1 tccaccacct gagccgcgat caccgtctcc ggggtggtcc ctggtccggg cgcaacttgt
       61 tctcctcctc ccattgcact ttcagcttgt ggaggatcgg cgccagacca tcgcttggcg
      121 gtggcgcggg cgcccgggcc agtgtgtcgc cattcagcag gaagaaggct ggaatggtgc
      181 ggatccgtaa ctggtccatg atcacggcgt tgtccccacc gaagtaccac ggccagtcgc
      241 gatccggatg cgcgtgtaca taggcctcca attcctgcgg gctccggtcc aggctgatgc
      301 ccaccacgtt gatcacctgc ccgtattcct tccggagcgt ctcgagcgcc accagttcct
      361 gttcgcagta cgtacaccat gcggccgtga ccaccagaca ggttgctcct cgtaatacag
      421 tatctatcct ggcctgttgc ccggacaggt cgcgcaacac cagtggtggc agcctgccac
      481 cgggacgcat cgtggtcagg tcccacagca tgttggcggc gatccgcctg ttctccggat
      541 gcatggatgt ggcggccacc cggtccagga tgcccaggat gcctttgcgg tcgaaggtct
      601 tgccggggta ttgggcatgg agtcccatca gcatcaccag ctcgcacagt gcatcgtcct
      661 tcaggagatc gtgttcggcg aagagtgttc tcacgctgtc cggatccgca gcacgcacgg
      721 cgttcaccag ggccgcttcg ttcttccgga acggataccg catgatgtgc tcctcgaaaa
      781 aagcctggat gaaccgcacg tactccggca cgtcgtagag cacggggcgg cccttcaggt
      841 atttcccgaa cagttcgcga tcgttcaccc cggggccgaa acgcaggccc gcgataccgt
      901 attccaggtt ttgaaggaac caggggtccc gcaccccgtt gtagtaacct tgtagtttca
      961 atgcgaaact gtcggtccgg gccgggctct tcgtcgggct caggaacagc gtctccgggc
     1021 gcccggcggt gtccttgccg agccgtcggc tcgtggccac ctcctccatg gcgaagtcgt
     1081 ccaatcgctg gttcaggtcg gccagcaacg cgttcacgtc catccggtcc agatccctga
     1141 attcgaggat ggtctccgtg gtgccgttca agctgcgtgg cgtaccgggc ggtggcggcg
     1201 gaaaggtgat gcggtacgtg cccggacgca gccacagacc cccatgtatc ccaccgatcc
     1261 tcaattgggc cttcaccgtg cccgtcacgt tggcctcgat cacggccttg ccgtcggcat
     1321 ccacctcggc ctgcccgatc agttcggtgc gcaaggtgaa caggtccagg tagcggtaca
     1381 acagcgcccg ctcccccgcg tgcaccgccg ccgtcagttc gatccgcaca ggtgcggcat
     1441 gcgcatgcgg gcataacgcg caccaggcga ccagggcgat caggatcctc acgatcgggt
     1501 gttgtccggg tccggccatc gcggcttcag ggccgtaccg ccccgggtac gaacgcggaa
     1561 acatccgctt tgttggcgat cagttcccgc acgatggtac tgctgatgtg cgcgtgctcg
     1621 ggcagggccg gcaggaagat cgtgtctaca cccgccagta cgtggttcat cagggcgatg
     1681 ctgcgctcgc tgccgtgatc cgtactgttg cgcaagccac gcacgatgta gcgcgctttc
     1741 atcctcttgc acaagtcggc cgtgaggccc tcgaaggcca tcacctccac ctgcttgtga
     1801 ccaacgaagg tggcgcgcag ccagcccagc cgttgctcta ccgggaacat gtattttttg
     1861 gtcgtgttca cgccgatggc cagcacgatg cggtcgaaca acggcaaagc gcgttccacg
     1921 atgctcacat gcccgatggt cacggggtcg aacgacccgg gaaagacggc gatgcgctcg
     1981 ctcattggcg ttcggcgttg atcaccccgg cttgcgccgc gggcgaaggt ttgaagaagc
     2041 tgaaatgcac gttcccgtac ggacggtgcc ggtcgaacca cggatcggtg ctcatgttca
     2101 cgttcttcgg gtgctcgatg atcagcaggc cctccggatc gagcagcccg gcttccctga
     2161 tcaggacagg caactgctcc gtcccttcca ggttgaacgg cggatcggcg aagatgatgt
     2221 cgtaggtggc gtgatggcct ttcacgaagt tgaacgcgtc ggctttcacg tggtgccaat
     2281 tggtcgcttg aagcgcccgg gccgcgccct gcaggtggct gaagagctcg cgatcctgtt
     2341 caacactgat cacctccccg gccttacggc tgaggaactc caacgagatg ctgcccgtgc
     2401 ctgcgaacag gtccagtacc cggataccct ccaacggcac ggtgtgctgc aggatgttga
     2461 agagaccctc cttcgcgtag tcggtggtag gtcgcgccag cgtgctcctg ggcggatgga
     2521 tccgccgccc cttgtatttg ccgcctacga tgcgcatgct tgctgttcga gcaacgctgt
     2581 gaaacggtgc ggccggtcca ggtccagccc cgccaggttc gggtccgacg gtgtgatcgc
     2641 gggcccgata tcctggaggt attttttcag caggtcctcc tcggtgccgg tgaggtgcgt
     2701 gccggcgatc aggccgcgga cgtcgtccgg gcgtgtgccg cattgttcca atgcgtagag
     2761 cacgtagtac agcacgtcct cgggcgtggc cgcgtggaag gtgttgctca gcagtagttg
     2821 ctggccgcgt gcgatggcca ggtcgagccg ctcggccgtt cggtggatca gagcggcggt
     2881 cttgtc
//
LOCUS       DAOQOC010000278         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18729_length_2872_cov_3.195598, whole genome shotgun sequence.
ACCESSION   DAOQOC010000278 DAOQOC010000000
VERSION     DAOQOC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18729_length_2872_cov_3.195598"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..1071)
                     /locus_tag="PL106_05585"
     CDS             complement(454..1071)
                     /locus_tag="PL106_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA32574.1"
                     /translation="MLVKRLRALPVGSAATSDIQLVNAYIAGDESAFEALLHRHKRKV
                     WSHIYLMVRDREVTEDLFQETFIKVVHHLKAGRYNEEGKLVAWVIRIAHNLVIDHFRR
                     NKKMPAVRSTEEHDVLGGIPQPGLNVEQRMVNAQVDDDVRKLIDHLPAEQREVVIMRT
                     YLNMSFKEIAEHTDVSINTALGRMRYALINMRKLVQQHGMELERA"
     gene            1570..2733
                     /locus_tag="PL106_05590"
     CDS             1570..2733
                     /locus_tag="PL106_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNA32575.1"
                     /translation="MDRATQVAVIGAGTMGSGIAQVAAQAGHPTLLYDARPGAVDQVL
                     GALRKTLDKLVEKGKLTADQANNVHDRIHPAGALSDLAGCGMVVEAVVEDLAVKKKLF
                     TELEALVTADAVLATNTSSLSVTAIAAACTRPERVLGLHFFNPAPLLPLVEVVPGLAT
                     STDLAARTADLMRAWGKTPVLCKDTPGFIVNRVARPFYGESIRIFEEGIADMPTIDAA
                     LKGAGFRMGPFELMDLIGNDINFTVTRTVWESFFYDPRYKPSITQQRQVESGRLGRKS
                     GRGYYNYTDGAVVPTLTVDPDLATAIVERVLAMLINEAADALFWGVASAADIDLAMTK
                     GVNYPKGLLAWARERGEAHWLAVLERLQADHGEDRYRPSPLLRRAVSQGKSLS"
BASE COUNT          535 a          875 c          870 g          592 t
ORIGIN      
        1 atgtatggcc atacatacgt tctgtggtcg ttcatcgaag gacgcatctc ccgcttcagc
       61 cgagcccccc gcccacatct accacatcga gcttcccaca tgggggtgat ccctcttcct
      121 gtggaacagc tggggcgtcc gactgagacc cgcgttccac gaaggcgaac gttccttgga
      181 cgccctttga gaacgtctgt cgattccagg agctccaata cccgtatttc tgtgttctcc
      241 ttgaacctca aagcggatcc atcgtgcagg ttcgacgagg gttgttactt ggttcatgtt
      301 ccacctgtaa agcctgacca cgacccacgt ggggatccct aacgatcgaa agcacgaacg
      361 tcggcttacg ggccgacgtt cctgggacga tgaggctctg atggcccgca ccatgccccg
      421 acgcgtcggg acggagtgcg acgcacggag agctcaggcc ctttccagtt ccataccgtg
      481 ctgctggacc agcttgcgca tgttgatcag ggcgtagcgc atccgcccga gcgccgtatt
      541 gatggacaca tccgtgtgct cggcgatctc cttgaaactc atgttcaggt aggtgcgcat
      601 gatcaccacc tcccgctgtt cagcgggaag atgatcgatc agcttgcgca catcatcatc
      661 cacctgggcg ttcaccatgc gctgctccac gttcagcccg ggttgcggga tgccacccag
      721 cacatcgtgc tcctcggtgc tgcgcacggc cggcatcttc ttgttgcgcc ggaaatggtc
      781 gatcaccagg ttgtgcgcga tccggatgac ccaggccacg agcttcccct cctcgttgta
      841 ccgcccggcc ttcaggtggt gtaccacttt gatgaaggtc tcctggaaga ggtcctccgt
      901 gacctcgcga tcgcgaacca tcaggtagat gtgcgaccac accttccgct tatggcggtg
      961 cagcaaggcc tcgaacgcgg actcgtcgcc cgcgatgtag gcattcacca gttggatgtc
     1021 ggaagtggct gcgctgccga ccggcagggc gcgaagacgc ttgacgagca tgtcaccgta
     1081 ccttttatcc gaaggtggtt caggaacttc ggggtagatg ttgctctatg cgatcttccg
     1141 ggttttgttc gtgttgcgca acgtgcgccg tggcaagata ctggacgtgg cttgggaacg
     1201 aaacgttgcc tggtggcgat ctattgtgtg ctcgccccta tccgatgtca aatataggga
     1261 agatcggcag gctccgctgt acctgaggaa caatatgaca cacacaaccc gttacgcgtc
     1321 gcctcgccgt gggctcgctc cgctccgatc catgaacggg aataaatcga acccggtggt
     1381 cttcccccct gatgagccgg tgacgaagaa gtggtcgttc catggttgga cagggcgaga
     1441 atgcaggggt caaagcggcg ccactcgcat tcgtgcgctc atgcacccga acggttccca
     1501 tgtccggtgc gtgcggatct tcgtcattgc cccagtaaaa tctgaccggc acatccgaac
     1561 ttcgccccga tggacagagc aacgcaggtg gccgtgatcg gcgcgggcac catgggcagc
     1621 ggtatcgcgc aagtggcggc gcaggccggg caccctaccc tgctgtacga tgcccgaccg
     1681 ggtgcggtgg accaggtctt gggcgctttg cgcaaaacgc ttgacaaact ggtggagaaa
     1741 ggcaagttga ccgctgatca ggccaacaac gtccatgacc gtatccatcc tgctggtgcg
     1801 ttgagcgacc tggccgggtg cggcatggtg gtggaggctg tcgtggagga tcttgccgtg
     1861 aagaagaaac tcttcaccga actggaagcc cttgtaacag cggatgccgt gctcgccacg
     1921 aatacctcgt cgctgagcgt taccgccatc gctgccgcat gcaccaggcc cgaacgcgtg
     1981 ctgggcctgc acttcttcaa cccagcgccc ctgctgcctc tggtggaagt ggtgcctgga
     2041 ctggccacct cgaccgatct ggccgcacgt accgcggacc tgatgcgcgc ctggggcaag
     2101 acacccgtcc tgtgcaagga cacgcccggc ttcatcgtga accgtgtggc ccggccgttc
     2161 tatggcgaaa gcatacgcat ttttgaggag gggatcgccg acatgcccac catcgatgcc
     2221 gccctcaagg gtgcgggctt ccgcatggga cccttcgagt tgatggacct gatcggcaac
     2281 gacatcaact ttacggtcac acgaacggtg tgggagtcct tcttctacga cccgcgctac
     2341 aagccgagta tcacgcagca gcgccaggtg gagagcggac gattgggacg caagagcgga
     2401 cggggctatt acaactacac ggatggtgcc gtcgttccaa ccttgactgt ggatccggat
     2461 ctggcaacgg ccatcgtgga gcgcgtgctc gccatgctca tcaacgaggc ggccgacgcg
     2521 cttttctggg gtgtggcctc ggcggcggac atcgatctgg ccatgaccaa gggcgtgaac
     2581 tatcccaagg ggctgctcgc ttgggccaga gagcggggtg aggcgcattg gctggcggtg
     2641 cttgaacgtc tgcaggccga tcatggggag gaccgctatc ggccttctcc cctgcttcgt
     2701 cgggcagttt cgcagggcaa gtccctttct tgaactggtt caagcgcgcg gatccggtgc
     2761 cgcacttatt tcgcggccca aacaaaaacg cgcacatacg catgaaaccg ttcgtagccc
     2821 ttactgccgt tgcggccgtg atgagcattg tagcctgtgg tccttccgag gc
//
LOCUS       DAOQOC010000279         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18762_length_2870_cov_4.327886, whole genome shotgun sequence.
ACCESSION   DAOQOC010000279 DAOQOC010000000
VERSION     DAOQOC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18762_length_2870_cov_4.327886"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="PL106_05595"
     CDS             complement(<1..1705)
                     /locus_tag="PL106_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNA32576.1"
                     /translation="MKHRSTLLLRSFTLAALLGTVALFIAWSSGSVPKAHAFHTEEEL
                     AAFGRGAGLATGSNTYFKGSGTCSGCHGHDPLAFAMVTQDGGEDVNVVDDWRSTMMAN
                     SAKDPFWRAKVSHEVLLDPTHQSELEDKCTSCHAPMGRHDKFLTGMGHYSMGELAQDM
                     LGQDGVSCVACHIQSADSIGLHFSGNLRFDTLNHPLYGPFGGPNDSVPIFGAPMTAFV
                     GYEPQYGAHINDAGLCAGCHTLQTDVVDLNGQPTGQVFTEQATYHEWLNSSFNTDEHP
                     EEGITCQGCHLPLIANDGVVLSANYMFLTPRSPFGRHHFSGANTFMLEMLKNNIGTLG
                     LFANETQFDSTINRTERMLRSSLVLDARMVSRDADTAFVDVTLLNTAGHKFPSGYPSR
                     RAWVELVLTDEGGDTLFRSGGWNDTWEVIGHDAQWEPHYDVITQPDQAQIYEMVMADV
                     NGDKTTVLGRSSVKLKDNRLVPLGFTVGHYSYDTSYIANVPVSDTDFNHDELGVEGSG
                     SDIIHYHAPMGGYAGNVNVQVNVWYQSAPPKWMEEMFGEHTPEIDAFRDMYDAADNTP
                     FL"
     gene            complement(1901..2461)
                     /locus_tag="PL106_05600"
     CDS             complement(1901..2461)
                     /locus_tag="PL106_05600"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNA32577.1"
                     /translation="MDLSKIISITGKSGLYRVIAPGRQAIIAESLEDGKRLAVPSSVR
                     VSSLDEISMFTTGDDEPLKNILEKLYKNAQGGASADPKSDEASLWKTLTEALPNADKE
                     RIYPSDVRKLFLWYTQLLKAGVFAEKDEKTEDDGEEKPKEGSKSKKSAGKATSASTGT
                     GTKKASTKPSGASKATAVRRGGQRGA"
     gene            complement(2506..2790)
                     /locus_tag="PL106_05605"
     CDS             complement(2506..2790)
                     /locus_tag="PL106_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA32578.1"
                     /translation="MTIEHRSGGLKDRFVALEDNMELGEMTFSMADKDKLIIDHTEGF
                     PGSEGKGVGLKLLEAAVSFARSKGLRIVPQCPFALKMMDRRKEEYADVRA"
BASE COUNT          523 a          888 c          839 g          620 t
ORIGIN      
        1 ccaggaaagg cgtattgtcg gcggcgtcat acatgtcccg gaaggcgtcg atctccggag
       61 tgtgctcacc gaacatctcc tccatccatt tcgggggggc actctggtac caaacgttca
      121 cctggacgtt cacgttaccc gcgtatccgc ccatcggcgc gtggtagtgg atgatgtccg
      181 aaccgctgcc ctccacgccg agttcatcgt ggttgaaatc cgtatccgac acgggaacgt
      241 tggcgatgta gctcgtgtcg taggaatagt gcccgaccgt gaagcccaat gggacaagcc
      301 ggttatcctt gagcttgacg gacgaacgcc cgagcacggt ggtcttgtcc ccgttaacgt
      361 cggccatcac catctcgtag atctgcgcct ggtcaggctg ggtgatcacg tcgtagtggg
      421 gttcccactg cgcgtcgtgg ccgatcacct cccaagtatc attccaacca ccgctccgga
      481 acagcgtgtc gccaccctca tcggtaagca cgagttcaac ccatgcccgg cgcgaggggt
      541 aaccgctcgg gaacttatgg ccggcggtgt tcaacaaggt cacatccacg aaagcggtat
      601 ccgcatcgcg tgacaccatg cgcgcatcga gcaccaggct gctgcgcaac atgcgctccg
      661 tgcggttgat cgtgctgtcg aactgggtct cgttggcgaa gagccccagc gtgccgatgt
      721 tgttcttcaa catctccagc atgaaggtgt tggccccgga aaagtggtgg cgaccgaagg
      781 gcgagcgcgg cgtcaggaac atataattgg ccgagagcac cacgccgtcg ttggcgatga
      841 gcggaaggtg gcatccctgg caggtgatcc cctcctccgg atgctcatcg gtattgaaac
      901 tcgaattcaa ccattcgtgg tacgtggcct gttcggtgaa gacctgaccc gtgggttggc
      961 cgttcaggtc gaccacatcg gtctgcaagg tgtggcaacc cgcgcacagg cccgcatcgt
     1021 tgatatgggc gccgtattgc ggctcgtagc ccacgaaagc ggtcatgggc gcgccgaaga
     1081 tcggaacgct gtcattcggc ccgccgaatg gcccgtacaa cgggtggttc aaggtgtcga
     1141 aacgcaggtt gcccgagaaa tgcaggccga tgctgtccgc gctctggatg tggcacgcca
     1201 cacagctcac cccatcctgc cccagcatat cctgcgcgag ctcgcccatg gaatagtgcc
     1261 ccatgccggt gaggaacttg tcgtgccgcc ccatgggcgc atggcaactg gtacatttat
     1321 cctccagttc ggactggtgc gttggatcca gcaggacctc gtggctcacc ttggcccgcc
     1381 agaacgggtc ctttgcggag ttcgccatca tggtgctccg ccaatcgtcc accacgttca
     1441 catcctctcc accatcctgc gtgaccatgg cgaacgccag cgggtcgtgc ccatgacagc
     1501 cgctgcaggt cccacttccc ttgaaatacg tatttgaacc cgtggccaga ccggcaccac
     1561 gaccgaaggc ggccagttcc tcctcggtat ggaacgcgtg ggccttcggc accgaaccgg
     1621 aggaccatgc gatgaacagg gccaccgtgc ccagcaaggc ggcaagtgtg aatgaccgga
     1681 ggagcagagt ggagcggtgt ttcatgcctt gtgtactgga tccaggtcca ttatgttgta
     1741 cgatcgtgtt gccgcaagat aggttcgacc ggacgcgtgg gcaaacccgt tcgcgaaaga
     1801 aaggatcctg gggtcgccgt gaaccggact gataaaaatc atgcgtcctt ccctgagaag
     1861 gcgctcatag gatcctcctc agctgcctcc actgagcgcg ttatgcccca cgttggccac
     1921 cccgccgtac ggcggtcgcc ttgctggctc cggaaggctt ggtgctggcc ttcttggtgc
     1981 ctgttccggt gctggcactg gtggccttcc ccgccgactt cttggacttg ctgccctcct
     2041 tgggcttctc ctcgccgtcg tcctccgtct tctcgtcctt ctctgcgaaa accccggcct
     2101 tcagcagttg cgtgtaccac aggaacagtt tgcgcacgtc gctgggatag atccgttcct
     2161 tgtccgcatt gggcaatgct tcggtaaggg tcttccatag cgaagcctca tcgctcttgg
     2221 ggtcggccga ggccccaccc tgggcatttt tgtagagctt ttccaggatg ttcttcaacg
     2281 gctcgtcatc cccggtggtg aacatgctga tctcgtccag cgaactcaca cgtacgctgc
     2341 tgggcaccgc cagacgttta ccgtcctcca gcgattcagc gatgatggcc tgacggcccg
     2401 gggcgatcac gcggtacaag ccgcttttgc ccgtgatgga gatgatcttt gagaggtcca
     2461 tgttgaggga tgacggggcg aatgtagggt gctgtgggct cggcttcacg cccgtacatc
     2521 ggcgtattcc tccttgcgcc ggtccatcat cttcagggcg aacgggcatt ggggcacgat
     2581 ccgcaggccc ttgctgcgtg cgaaggatac tgcagcctcc aacaatttca ggcccactcc
     2641 ttttccctcc gaacccggga aaccctccgt atggtcgatg ataagcttgt ccttgtcggc
     2701 catggaaaag gtcatttcgc cgagttccat attgtcctcg agcgcaacga aacggtcctt
     2761 caagcccccc gatcgatgtt cgatggtcat gatgcgaagt acgtttgata ccactccttt
     2821 tcccagtgat cgcgctcatc ggtcaatgtg tgcgtgccgt ccaacaggtc
//
LOCUS       DAOQOC010000280         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18818_length_2866_cov_3.230167, whole genome shotgun sequence.
ACCESSION   DAOQOC010000280 DAOQOC010000000
VERSION     DAOQOC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18818_length_2866_cov_3.230167"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1085)
                     /locus_tag="PL106_05610"
     CDS             complement(99..1085)
                     /locus_tag="PL106_05610"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA32579.1"
                     /translation="MRIAMTGANGHIGTVLSTLMHGAGHELCLLAHQRTDGIGHLGAE
                     LVRGDVLDPAAMDRLVRDCEVVVHLAARISIDSDNDPLVRPVNVDGTRNVVEACLRNG
                     VGRLVHFSSIHSYNAHPRNEPLDELRAPTAATSPTYDRSKASADVVVLDAVRDKGLDA
                     VILAPTSVFGPMDHGPSLLGRAIADLHNGRIPLLPPGGYDFVDVRDVAAAAMSALTRG
                     ATGQKYLLSGQYRTVRELAAAIGRITGKRVVQRVMPASVLRALIPFFRVQARLAGRTP
                     LVTHEALNALTEGHPDIRNTKARAELEFRPRGFEETLTDTLEWMRGAGLLNG"
     gene            complement(1087..1830)
                     /locus_tag="PL106_05615"
     CDS             complement(1087..1830)
                     /locus_tag="PL106_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNA32580.1"
                     /translation="MDVFTLAVIAWMLVAVVTMPVLLRVRAPYGRHVKPGWGPTVDNR
                     WGWFIMELPALVLMPLLTILGPRDKDTLTWLLTGLWCAHYVNRTLIFPFRLHTRGKRM
                     PLAIVLSGIGFNAINGGLNGWWLGFTAPPDRGFPDVLSVLGIVLFAAGMGINHWADTR
                     LIDLRAIGDGYQIPRGGPFERISCPNHFGEILEWWGFALAAWSLPSLSFAIWTFCNLA
                     PRARNHHAWYREHFPDYPPGRRAVIPFIW"
     gene            1887..2708
                     /locus_tag="PL106_05620"
     CDS             1887..2708
                     /locus_tag="PL106_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNA32581.1"
                     /translation="MPTSSRTLIAGIGAKDHLCAMKLISFNVNGIRASVGKGLHATLH
                     QLDADIVCFQETKATPEQVATALGGVNGYHVHANGALKAGYSGTAIASKEKPLDVQRG
                     IGVNEHDDEGRVVTCVFKDVVTVCVYTPNSGEGLKRLSYREKWDAAFRAYVNNLATGK
                     LPVIVTGDLNVAHQPIDIARPKENYNKTSGYTQQEIDGLSSLLDTGWVDTFRHLHPGV
                     VKYSWWSQRLGARAKNVGWRIDYVLVNKGFEKQVKNAFILNEVMGSDHCPVGIEW"
BASE COUNT          568 a          940 c          870 g          488 t
ORIGIN      
        1 ttcggcaggc tcaccgtgac aagtcttttt cattcctctg ggcaattggg acggatgtct
       61 attgctatgg ccggccgctc ccccgaatgg gggatatgct atccgttcag caggccggct
      121 ccccgcatcc attccagggt atcggtcagc gtctcctcga aaccgcgcgg ccggaactcc
      181 aactccgccc gggccttggt gttgcggatg tccggatgcc cttcggtcag cgcgttcaac
      241 gcctcatgcg tgaccaacgg tgtgcgcccg gcgagacgtg cctgcacacg gaagaagggg
      301 atcagtgcgc gcagcacgct ggcgggcatc acccgttgaa ctacccgttt cccggtgatg
      361 cgaccgatgg ccgccgccag ttcccgcacg gtgcggtact ggccactcaa gaggtacttc
      421 tgtcccgttg cccccctcgt cagcgcgctc atcgcagcgg cggctacatc acgcacgtcc
      481 acgaagtcgt atcccccggg cggaagcagt ggtatgcgcc cgttgtgcag gtcggcgatg
      541 gcacggccca gcaacgaggg tccatggtcc atcgggccga acaccgaggt gggcgccagg
      601 atcaccgcgt ccagtccttt gtcccgcacg gcatcaagca ccaccacgtc cgccgacgcc
      661 ttgctgcgat cataggtcgg tgaggtggcc gcggtgggag cccgcagttc gtccaagggc
      721 tcgttccgtg ggtgggcgtt gtagctgtgg atgctgctga aatgcacgag gcgccccacg
      781 ccgttccgta agcaggcctc caccacgttg cgcgtaccgt ccacattgac cggacgtacc
      841 agtggatcgt tgtcgctgtc gatgctgatg cgcgcggcca ggtgtaccac gacctcgcaa
      901 tcccgcacca agcgatccat agcggccggg tccagcacat caccccgcac aagttcggcg
      961 ccgaggtggc cgatgccgtc ggtgcgctgg tgcgcaagca ggcacagctc gtggcccgca
     1021 ccgtgcatca aggtggaaag aaccgttccg atgtgcccgt tggcacctgt catggcgatc
     1081 cgcatgctac cagataaaag gtatcaccgc ccttcgcccc ggcggataat cgggaaagtg
     1141 ctcacggtac cacgcatggt ggttccgcgc gcgtggggcc aggttgcaga aggtccagat
     1201 ggcgaaggag agcgatggca acgaccatgc ggccaaggcg aagccccacc actccaggat
     1261 ctcgccgaaa tgattggggc aactgatgcg ctcgaacggt cccccgcgtg ggatctgata
     1321 gccgtcgcca atggcccgca ggtcgatcag tcgggtatcg gcccagtgat tgatccccat
     1381 gcctgcggcg aagagcacga tgcccaacac cgacagcaca tccgggaagc cccggtcagg
     1441 tggggccgta aaacccagcc accagccatt cagcccccca ttgatggcgt tgaatccgat
     1501 gccgctgaga acgatggcca gcggcatgcg cttgccccgc gtgtgcaacc tgaacgggaa
     1561 gatcaacgtg cgattgacgt agtgtgcgca ccagaggccg gtgagcagcc aggtgagcgt
     1621 atccttatct cttggaccca ggatcgtgag caagggcatg agcaccagtg cgggcagttc
     1681 catgatgaac cagccccagc ggttatcaac cgtgggaccc caaccgggtt tgacgtgccg
     1741 accgtacggc gcccgtacgc gcagcaacac cggcatcgtc acaacggcca ccaacatcca
     1801 tgcgatcacc gccagcgtga atacgtccat gcggcgatga tacaccagcg gcacgatccg
     1861 caacctccaa gagccccatc gctcccatgc ccacatcctc ccgcacgctc atcgccggga
     1921 tcggagcgaa ggaccacctt tgcgccatga agctgatctc tttcaacgtc aacggtatcc
     1981 gggccagtgt gggcaagggt ctgcacgcga cactccacca gttggacgcc gacatcgtct
     2041 gtttccagga aaccaaggcc acccccgagc aggtggccac cgcgttgggc ggggtgaacg
     2101 gttaccacgt ccacgcgaac ggtgcgctca aggcaggata cagcggtacg gccatcgcca
     2161 gcaaggaaaa gcccctggat gttcagcggg ggatcggcgt gaacgagcat gacgacgagg
     2221 gccgcgtggt cacctgcgtg ttcaaggatg tggtgacggt ctgcgtgtac accccgaaca
     2281 gcggcgaagg attgaaacgc ctcagctatc gggagaaatg ggatgccgcc ttccgcgcat
     2341 acgtgaacaa cctggcaaca gggaagctgc ccgtcatcgt caccggcgac ctcaacgtgg
     2401 cccatcaacc catcgacatc gcacggccca aggagaacta caacaagacc agcggctaca
     2461 cccagcagga gatcgacggg ctctcgtccc tgctcgatac cggttgggtc gacacgttcc
     2521 gccacctgca ccccggggtt gtgaagtaca gctggtggag ccagcgcttg ggcgcacgcg
     2581 ccaagaacgt gggctggcgc atcgattatg tgctggtgaa caagggcttc gagaagcagg
     2641 tgaagaacgc cttcatcctc aacgaggtga tgggcagcga tcactgcccc gtcggtatcg
     2701 aatggtgaga aacgcgatcc cgttccgcta ccgctgaccg tccactcttc ctaccgatcc
     2761 gcaccgggaa atgaagtccc tcctccgcct cgaagaactc gcccagttcc tgctcatcct
     2821 gctcgtgctc ctggtgctgc agatctacac cgcagtcgag cgcttc
//
LOCUS       DAOQOC010000281         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18831_length_2865_cov_3.359075, whole genome shotgun sequence.
ACCESSION   DAOQOC010000281 DAOQOC010000000
VERSION     DAOQOC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18831_length_2865_cov_3.359075"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PL106_05625"
     CDS             complement(<1..1113)
                     /locus_tag="PL106_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32582.1"
                     /translation="MHTTAIMHREKLIDRLTQFAIWVCLGVALLLTLLMATSYRAAAQ
                     WSTDPANPMVVCNAPDQQRSMRAIADADSGWYVFWSDARNNAAKADLYGQHFDSDGNA
                     LWTANGELLLSDPARSINILEPLLLPDGSVMMAYGTHAQINFMDTIRAMRFDVDGSAL
                     WPTSSILLTGLDYRTMEVVPSDSCLYLVAYCESCGGGGYGYRMQRVRMDGTVQFPTLG
                     QPASGYWGSFSIHPDDVGGVLFNVRCGNGAGTCLKAQRFDTLGTAVWQADIDLADPDG
                     LSYAFTTSQDGSGAETAVWEVNGDLRMRRIDTTGTSLWSPGVLPACALPTYSQALPAI
                     LTMGTELFVAWQDNRPPANNVDLYIQKFDLTTGTALW"
     gene            complement(1212..2054)
                     /locus_tag="PL106_05630"
     CDS             complement(1212..2054)
                     /locus_tag="PL106_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA32583.1"
                     /translation="MPLRPIFQVRGERVQRVLYLADHTHGAEPVQRPMGAIKAVIVDD
                     SADNRDYLRTLLASAHPHVRTAGEAANIQEALLAIARHSPDLVFLDVEMPGGSGFDLL
                     RKLEQWPFEVIFCTAYERYAVQAIRFSALDYLLKPVQPDELAEALARFEQRRAIEHRS
                     AVQRQFLSNLGAPDEQAMKLTLTTGDRTWFVSPAELTHCTADDNYTELHTLDGRRFVS
                     ARTLKDYEDMLAPLGFLRVHRSSLVNRSAITHLDDGHVVLKNSARVEISRRKKEEVLK
                     ALAG"
     gene            complement(2150..>2865)
                     /locus_tag="PL106_05635"
     CDS             complement(2150..>2865)
                     /locus_tag="PL106_05635"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA32584.1"
                     /translation="FLQKQKHANVLEKLNQQLADSNKEVTEINGLLEMKLLRSQMNPH
                     FIYNCLNSAAQMTQAGKQVEALAYLQGFARLLRMVLDHSVNDTVSVEEEVDFLLQYLK
                     LEAHRLPDFTYDVNAAPDLLDDGAIIPALIIQPFVENAVWHGLAHKVGDRSITVHFTQ
                     GSDGPVCTVTDNGIGRKEATSAHHAQDPSHKSLGMQLTSERLKLMARRMQRDGSITIE
                     DLQAPDGSAAGTRVTVVLR"
BASE COUNT          535 a          845 c          917 g          568 t
ORIGIN      
        1 ccatagcgca gtaccggtgg tcaggtcgaa tttctgtatg tacaggtcga cattgttcgc
       61 gggcgggcga ttgtcctgcc acgccacgaa cagctccgtg cccatggtca ggatcgcggg
      121 cagggcctgt gaataggtgg gcagcgcgca tgcgggcagt acgccgggcg accatagcga
      181 tgtgcctgtg gtgtcaatgc ggcgcatacg caggtcgccg ttcacttccc acaccgccgt
      241 ttccgcacct gatccatcct gcgatgttgt aaaggcatag ctgaggccgt ccggatcggc
      301 caggtcgata tcggcttgcc acaccgcggt tcccaacgtg tcgaagcgct gcgccttcaa
      361 gcaagtacct gcgccgttac cgcaccggac attgaacagg accccaccca cgtcatcggg
      421 gtggatcgaa aaggaccccc agtatcccga ggcgggttgc ccgagagtgg ggaactgcac
      481 cgtcccatcc atgcgcaccc gttgcatccg gtagccgtag ccaccaccac cacaactttc
      541 gcagtaggcc accaggtaca ggcaactgtc gctcggcacc acctccatgg tccggtagtc
      601 caagccggtg agcaggatgg aggatgtagg ccacaaggcg gatccgtcca catcgaaacg
      661 catggcacgt atggtgtcca tgaagttgat ctgcgcatgg gttccatagg ccatcatcac
      721 cgagccatcc ggaaggagca gcggctccag gatgttgatg ctccgtgcag gatcgctgag
      781 caacagttca ccattggcgg tccacaaggc gttgccatcg ctgtcgaagt gttgaccgta
      841 gaggtcggcc ttggcggcgt tgttgcgcgc atcgctccag aacacgtacc agccgctgtc
      901 ggcatcggcg atggcccgca tggaacgctg ctggtccggc gcgttgcaca ccaccatggg
      961 attggccgga tcggtgctcc attgggctgc ggcgcggtag gaggtggcca tcagcagggt
     1021 caacagaagg gccacaccaa ggcagaccca gatggcgaat tgtgtcaggc ggtcaatgag
     1081 tttctcgcgg tgcatgatgg cagtggtgtg catgtggatc atgtggttcg gcattgtgac
     1141 cccgaatcaa gaactgcttt cgcctgcgag aaaggaactc cgagcaaacg gtcgggtggg
     1201 cgagggagcg gtcacccagc gagtgctttc agcacctcct ccttcttcct cctgctgatc
     1261 tccacgcgcg cgctgttctt cagcaccacg tgtccatcat ccagatgggt gatcgccgaa
     1321 cggttcacca gggaggagcg atgcacgcgc aggaagccga gcggtgcaag catgtcctca
     1381 tagtccttca gggtgcgggc cgaaacgaag cgacggccat cgagggtgtg gagttcggta
     1441 taattgtcgt cggccgtgca gtgggtgagt tccgccggcg aaacgaacca ggtacgatca
     1501 ccggtggtga gggtgagttt catggcctgc tcatcgggcg cacccagatt gctcaggaat
     1561 tgccgctgaa cggcgctgcg gtgttcgatc gcacggcgct gttcgaatcg ggccaacgct
     1621 tccgcgagtt catcgggttg cacgggtttc agcaggtagt ccaacgcgct gaagcggatg
     1681 gcttgtacgg cgtagcgttc gtaggcggtg cagaagatca cctcgaaggg ccattgctcc
     1741 aacttgcgca gcagatcgaa gccactgccg ccgggcatct ccacatcaag gaagaccagg
     1801 tcgggcgagt ggcgtgcgat ggcgagcaac gcctcctgga tgttcgcggc ttccccggcc
     1861 gttcgcacgt gcggatgggc cgatgcgagc agggtgcgca aatagtcgcg gttgtccgca
     1921 ctgtcatcaa cgatcacggc tttgatcgcc cccatcggtc gctgcaccgg ttctgccccg
     1981 tgcgtatggt cggcaaggta gagcacgcgc tggacgcgct cgccacgtac ttggaaaatg
     2041 ggacgaagtg gcacgggccg gtcgaaggat gttcggccga gaaggtcggt gcggcatcgg
     2101 gatcccggtc cgaacgtcca gtgggtggac ccaggaccga gcgaacggtt cagcgcagca
     2161 ccacggtcac ccgggtaccg gccgccgagc cgtccggcgc ctggaggtcc tcgatcgtga
     2221 tggagccgtc gcgctgcatc cggcgtgcca tcagcttcaa gcgttcgctc gtaagctgca
     2281 tgccgagcga cttgtgcgag ggatcctgtg cgtgatgcgc ggaagtggcc tctttccgac
     2341 cgatgccgtt gtccgtgacc gtgcagaccg gtccatcgga gccctgtgtg aaatgcaccg
     2401 tgatggaccg gtcgccgacc ttgtgggcca gaccgtgcca caccgcgttc tccacgaacg
     2461 gttggatgat gagtgcgggg atgatcgctc catcgtccag cagatccggt gcggcattca
     2521 catcgtaggt gaagtccggc aaccggtgag cttccagttt cagatactgg agcaggaagt
     2581 ccacctcttc ttccacggac acggtatcat tcacactatg atccaacacc atgcgcagca
     2641 agcgcgcaaa accttgcaag taggcgagcg cctccacctg tttgcccgct tgcgtcatct
     2701 gtgcggcgct gttcaggcag ttgtagatga agtgcgggtt catctggctg cgcagcagtt
     2761 tcatttcaag cagtccattg atctcggtga cctccttgtt gctgtccgca agttgctggt
     2821 tgagcttttc cagcacgttg gcgtgcttct gtttctgcag gaagt
//
LOCUS       DAOQOC010000282         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18839_length_2864_cov_3.569242, whole genome shotgun sequence.
ACCESSION   DAOQOC010000282 DAOQOC010000000
VERSION     DAOQOC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18839_length_2864_cov_3.569242"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PL106_05640"
     CDS             <1..220
                     /locus_tag="PL106_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32585.1"
                     /translation="VLTTPVGLTRVKKATDGAPNTDVLAYPVVSNTYPTLATPNKDVF
                     SVPDTEIPTSRVGVPKALTSSTAAGLTT"
     gene            274..1239
                     /locus_tag="PL106_05645"
     CDS             274..1239
                     /locus_tag="PL106_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32586.1"
                     /translation="MTVDGVPVFSASLEEVDFGLNRYANAYMDFGLFKDNDMHYNRCY
                     KLPNNKLKVYGAEAGQGRITVTSGEEKRVAIEAVDPNGNRSELRFTLRGVTAEKAATW
                     PMPTVQGQLFRYDRENTIANSTARFTLPPMSLYADERIVLSTDTTPGKAAAMRYAPLV
                     HLHDRLTPMHLAGQLAMKADVARAGKHADKLLIVRVDEKGKIKPVGGTLASDGWISTK
                     VKSFGDHTVMLDTLPPRVTAIGLKPVMTGRNSIILQVSDDLSGVDQWKGELDGRWILF
                     EYDPKQKMLIHHFDRYSDRPGEHELKMEVRDERGNTGKLTARFSR"
     gene            complement(1244..2422)
                     /locus_tag="PL106_05650"
     CDS             complement(1244..2422)
                     /locus_tag="PL106_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA32587.1"
                     /translation="MRRRDFLRSTGLVTSGLLAPRFLHGMVGALPLGGRKLVVVQLTG
                     GNDGLNTIVPLQNDDYHRLRPKLALAPGVALPVSDGLGLNPGMEAIRRLFDDGRVCVI
                     NGVGYPDPDHSHFRSMDIWQTASNSSEYLDTGWLGRYLDARSIAPHDVVEFGGTLSLA
                     NKGQRMKAITLTDPARFFQATREPWFAQLASVRHEEEHRELGLLYRTMVETYRSAGYI
                     HEHLRPRTTGITYPKGDLARQLQHVSAFIQSGMDTSVYYTSTGGFDTHVNQLVKHQKL
                     WTGVSDGIAAFLNDLRTAGLGKEVLVMVFSEFGRRAKENASNGTDHGTAGPVLLFGEG
                     LRKPGFFNPVPSLSRLDDNGDLVFSTDFRRIYATLLKDWLDTDPAAVIGGRFEKIPVL
                     "
     gene            complement(2422..>2864)
                     /locus_tag="PL106_05655"
     CDS             complement(2422..>2864)
                     /locus_tag="PL106_05655"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNA32588.1"
                     /translation="FEREEDPLFLQRLLGQELLNPPNVSGWKEGDGWVDSNALMLRLK
                     LPSALLNMGQLNWEDRGASTGDLDKMMSTPDTPMRDAKGRVFRTAPDKAAFLAQLPPN
                     ITNEDLFELMLQVPPTEVLRSSMGQGDLMERVLELLSSPEYQLC"
BASE COUNT          595 a          857 c          877 g          535 t
ORIGIN      
        1 cgtattaact acacctgttg gacttacaag agtaaagaag gctacggatg gcgcaccaaa
       61 cacagatgta ttggcatatc ccgtagtatc taatacatat ccaactctag ctacaccaaa
      121 taaagatgtg ttttctgtgc cagatacaga gattcctact tctagagtag gagtgccaaa
      181 agcattgact tcttctacgg ctgcgggact tactacataa gatacagacg gtacaccaac
      241 agcagcagca cctgcggcat tcgctccctg aaggtgaccg tggatggcgt gccggtcttc
      301 agcgcatcgc tggaagaggt ggatttcggc ctgaatcgct acgccaatgc gtacatggat
      361 ttcggcctct tcaaggacaa cgacatgcac tacaaccgct gctacaagtt gcccaacaac
      421 aagctgaagg tatacggtgc cgaggccggt caaggtcgga tcacagtgac cagtggcgag
      481 gagaaaaggg tggcgatcga agctgtggac cccaacggca accgcagcga actgcgcttc
      541 accctgcgcg gggtcacggc agaaaaggcc gccacctggc ccatgcccac cgttcaaggc
      601 caactcttcc ggtacgaccg cgagaacacg atcgccaatt ccactgcacg cttcaccctg
      661 ccgcccatgt ccctgtatgc cgatgagcgc atcgtgctga gcacggacac cacgccgggc
      721 aaggccgcgg ccatgcgata cgcacccctg gtccacctgc acgaccgtct aaccccaatg
      781 cacctggccg ggcaacttgc catgaaggcc gacgtggccc gggccggtaa gcacgcagac
      841 aaattgctga tcgttcgggt ggacgaaaag gggaagatca agccggtggg cggcaccctt
      901 gcgagtgatg gatggatcag caccaaggtg aagtccttcg gtgatcacac cgtaatgttg
      961 gacaccctgc ccccgagggt gacggccatc ggcctgaagc ccgtgatgac cggtcgcaac
     1021 agcatcatcc tacaggtgag tgatgacctg agcggggtgg accaatggaa aggcgaattg
     1081 gacggccgct ggatcctctt cgagtacgat ccgaagcaga agatgctcat ccaccatttc
     1141 gatcggtaca gcgaccggcc gggcgagcac gaactaaaga tggaagtgcg cgacgaacgc
     1201 ggcaacacgg ggaaactcac ggcccggttc agccgatgac atactacaat acagggatct
     1261 tttcgaaccg tcccccgatc accgccgcag gatccgtgtc caaccagtcc ttcagcaggg
     1321 ttgcgtagat gcgccggaag tcggtggaga acaccaggtc tccgttgtcg tccaatcggg
     1381 agagggacgg cacgggattg aagaagccgg gtttccggag gccctcgccg aagagtagca
     1441 ctggacctgc cgtgccatgg tccgtaccgt tgctggcgtt ctccttggcg cggcgaccga
     1501 attcgctgaa caccatcacc agcacttcct tgccgagtcc ggccgtgcgc aggtcgttca
     1561 agaaggccgc gatgccatcg ctaaccccgg tccacagctt ctggtgcttc accaactggt
     1621 tcacatgggt gtcgaaacct cccgtgctcg tgtagtacac actggtgtcc atgccactct
     1681 ggatgaacgc gctcacatgc tgtagttggc gggcgaggtc gcccttcggg taggtgatac
     1741 cggtggtgcg gggccggagg tgctcatgta tataacccgc actccggtac gtttccacca
     1801 tggtgcggta caggagtccg agttcgcggt gttcctcttc atgccggacg gaggccagct
     1861 gtgcgaacca gggttcgcgg gtggcctgga agaagcgggc gggatccgtg agggtgatgg
     1921 ccttcatccg ttggcctttg ttggccaggg acagcgtgcc gccgaactcc accacgtcat
     1981 gcggggcaat ggaacgggcg tccaggtaac gtcccagcca accggtatcg aggtactcgc
     2041 tgctgttgct ggccgtttgc cagatgtcca tgctgcggaa atgcgagtgg tccggatccg
     2101 gatagcccac gccgttgatc acgcacacac gtccgtcgtc gaagagtcgg cggatcgctt
     2161 ccatgccggg gttcaggccc aggccgtcgg acacgggcaa ggccactccg ggagccagcg
     2221 ccaacttggg ccgcagccgg tggtagtcgt cgttctgcag cggtacgatc gtgttgaggc
     2281 catcgttccc gccggtgagt tgcaccacca ccagtttccg tcctcccaag ggcaaagcac
     2341 ccaccatgcc gtgcaggaat cgtggggcca ggagtccgga ggtcaccagg ccggtgctgc
     2401 gcaggaagtc gcggcggcgc atcagcagag ctggtattcg ggtgaggaga ggagttcgag
     2461 cacgcgctcc atgaggtcgc cttggcccat gctggatcgc agcacctcgg tgggcggcac
     2521 ctggagcatc agttcgaaaa gatcctcgtt cgtgatgttc ggtggcaatt gagcgaggaa
     2581 ggccgccttg tccggtgcgg tgcggaagac gcgacctttg gcatcgcgca tgggcgtgtc
     2641 cggggtgctc atcatcttgt ccagatcgcc cgtgctggcg ccccggtcct cccagttcag
     2701 ttgccccatg ttcaacaggg ccgaaggcag tttgagtcgc agcatcaggg cgttgctgtc
     2761 cacccaacca tcgccctcct tccagccgga cacattgggc gggttcagta attcctgacc
     2821 cagcaggcgc tgcaggaaca gggggtcctc ctcacgctcg aaac
//
LOCUS       DAOQOC010000283         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18846_length_2863_cov_4.407407, whole genome shotgun sequence.
ACCESSION   DAOQOC010000283 DAOQOC010000000
VERSION     DAOQOC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18846_length_2863_cov_4.407407"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PL106_05660"
     CDS             <1..337
                     /locus_tag="PL106_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNA32589.1"
                     /translation="IEMTRQRVRPETEIDTSERCPTCAGTGEVSAPVLIIDEIENALN
                     FLHGEKNMSGLTLKVHPFLAAYFTKGLPSIRMKWWWRWKKGVKVVGEGSHQYLDYSIQ
                     DSEGKEVPL"
     gene            415..>2863
                     /locus_tag="PL106_05665"
     CDS             415..>2863
                     /locus_tag="PL106_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32590.1"
                     /translation="MKQIPVLALALVATPCVTSAQYVINDPGFLSDLQDAVPAALTGN
                     VLDTLHPDVISLSTLDASSSDDIDGIRFFVNLEALGLNYSTVDTLLELPNSLRSIDLD
                     WLEYRLRKVDRWPDSLRHLSFRNDWYWNPGTAQQDIGLIDTLPPLLEYLDLYGHTLDT
                     LPALPTTLRHLDVQRNLQLTALPALPNALLHLNVSQTDIPALPVFPDSLMYLSCGSNL
                     HLAMIGPLPAGLKELHVTTVVPMLPDLPALPEGLEVLEYGGTTLGSTIPTLPSTLREL
                     LLNNLNNVTVVPDLPHGLQRLQATAMNNVAQLPALPTTLTVLNTENSLFDCYPYVPSS
                     VTEWKVSTNTGCIPNLPPDLNNASNADDFPICTVVTSPDCPTTEGVTGHIFIDADGDG
                     VRDAGEAPFPAAMVLGTPGTHLAGADADGRYMLALDTGDFIVDGADLAYAQRTTAPYA
                     VHISAPLQVDSLVDIGYQLLPGASDLRVVLSASDARPGFGHVVQVTVVNDGPFGTDGT
                     LQVTLEPAFTYLSADPMPDDVNGNVLSWSLMNVLPGMSRHVHVQLNVPVDIPIGTPGM
                     HTAVLSIPDEDPTPGNNGAQLHFVVVGAYDPNDKRVIPSLMSMEEVQGGTPLEYTIRF
                     QNTGTYLAERVVITDTLQMGLMAGSSQFIGSSHACSWYLDDDVLVVIFDGINLPDSTS
                     DEPNSHGFVRFGIRPDPDLIAGDQVANTANIYFDFNEPVITVPAVFTVELQTPAREVD
                     APALLLAPNPAQDIIILRFPAPVANADLRVTDSAGRTVHQGLVSGTSSALSTGRWAPG
                     VYLVRLRGSNGLWAVRLV"
BASE COUNT          542 a          924 c          869 g          528 t
ORIGIN      
        1 gatcgagatg acgcgccagc gcgtgcgccc cgaaacggag atcgacacca gcgaacgctg
       61 ccccacctgt gccggcaccg gcgaggtgag cgcccccgtc ctcatcatcg acgagatcga
      121 gaacgcgctc aatttcctgc acggcgagaa gaacatgagc ggcctcacgc tcaaggtgca
      181 tcccttcctg gccgcctact tcaccaaggg gctgcccagc atccgcatga aatggtggtg
      241 gcgttggaag aagggcgtga aagtggtggg cgaaggcagc caccagtacc tggactattc
      301 gatccaggac agcgaaggca aggaggtgcc gctgtagggc gcgtccggca atgggtcaga
      361 aaccccgaaa gcaacggttg ggaagcgcac acgtctatgg ggcaagacta gcgcatgaag
      421 cagatccccg tccttgccct tgccctcgtg gctaccccgt gcgtcacctc agcacaatac
      481 gtcatcaacg atcccggctt cctgagcgat ctgcaggatg ccgtaccggc ggcgctcacg
      541 ggcaatgtgc tcgacacgtt gcacccggat gtgatctcgc tttccacgtt ggacgcgagc
      601 agtagtgatg acatcgatgg gatccgcttt ttcgtgaacc tcgaggcgtt ggggctcaac
      661 tacagcacgg tggataccct gctcgaactg ccgaattccc tgcgctcgat cgatctggac
      721 tggctggaat atcgccttcg caaggtggat cgctggccgg attccttgcg gcatttgagc
      781 ttccggaatg attggtactg gaacccgggg accgcccagc aggatatcgg cttgatcgat
      841 acgctgccac ccctgttgga atacctggat ctctacggcc acaccctgga cacgctgccc
      901 gcgttgccga ccaccctacg ccacctggac gtgcagcgga acctgcagtt gaccgccctg
      961 cccgcgctcc ccaatgcctt gctccacttg aacgtctccc agacggacat cccggccctg
     1021 ccggtgttcc cagacagcct gatgtacctg agttgtggga gcaatctcca cttggccatg
     1081 atcggccccc tgccggcggg cctgaaggaa ctgcacgtca ccaccgtggt gcccatgctg
     1141 ccggacctgc ccgcactgcc ggaaggcctg gaggtgttgg agtacggtgg caccaccctc
     1201 ggttccacga taccgacact gccgtccacc ctgcgggaac tgttgttgaa caacctcaac
     1261 aatgtgacgg tggtaccgga tctgccacac ggacttcaac gcctccaggc cacggccatg
     1321 aacaacgtgg ctcaacttcc ggcattgccc acgacactca ccgtgctcaa cacggagaac
     1381 tcgttgttcg attgctatcc ctacgtgccg tcctccgtca cggagtggaa ggtgtccacg
     1441 aacaccggct gcatccccaa tctgccgccg gacctgaaca acgcctccaa cgcggacgac
     1501 ttcccgatct gcacggtggt caccagcccc gactgcccca ccaccgaagg cgtgacgggc
     1561 catatcttca tcgatgccga tggcgatggg gtccgcgacg cgggcgaagc gcccttccct
     1621 gcggccatgg tgttgggcac cccgggtacg cacctggccg gtgccgacgc ggatggacgc
     1681 tacatgctcg cgctggatac cggcgatttc attgtggatg gagcggatct ggcgtatgcc
     1741 cagcggacca cggctcccta tgcggtacac atcagcgccc cgctccaggt ggacagtctg
     1801 gtagacatcg gataccaatt gcttccaggt gcgagcgacc tccgtgtggt cctttctgcg
     1861 agcgatgcgc gacccggatt cgggcatgtg gtgcaggtga cggtcgtgaa cgacggaccc
     1921 ttcggaacgg atggcacctt gcaggtcacc ttggagcccg ctttcaccta cctgtcggcg
     1981 gatccgatgc cggatgacgt gaacggcaat gtgctgagtt ggtcactcat gaacgtgctg
     2041 ccgggtatga gccgccatgt gcatgtgcaa ctgaacgtgc ccgtggatat accgatcgga
     2101 acgccgggca tgcacactgc ggtcctgtcc attccggatg aggacccgac gccagggaac
     2161 aacggcgcac agctgcactt cgtagtggtg ggcgcgtacg atccgaatga caagcgcgtg
     2221 atcccgtcgt tgatgagcat ggaagaggta cagggtggca caccgctgga gtacaccatc
     2281 cgcttccaga acacgggcac ctacctcgcc gagcgcgtgg tgatcaccga taccctgcaa
     2341 atgggactca tggcgggcag ttcgcagttc atcggcagct cccacgcctg ctcgtggtac
     2401 ctggacgacg atgtcctggt cgtgatcttt gatggcatca acctgccgga cagcacgagt
     2461 gatgagccga acagccatgg cttcgtgcgc tttggcatcc gtccggatcc ggacttgatc
     2521 gcaggtgatc aggtggcgaa cacggcgaac atctacttcg acttcaacga accggtgatt
     2581 actgttcctg ctgttttcac cgtagaactc cagacgccgg cgcgggaggt ggacgccccg
     2641 gcgctactgc tcgcgccgaa tccggcgcag gacatcatca tcctgcgctt ccccgcgccg
     2701 gtggccaacg cggacctccg ggtgaccgac agcgcaggtc gcacggtgca tcagggcctc
     2761 gtcagcggaa cgagcagcgc gctcagcacc gggcgctggg cgcctggcgt gtacctggtg
     2821 cgactgcgcg gctccaacgg gctttgggcg gtgcgtctgg tga
//
LOCUS       DAOQOC010000284         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18903_length_2859_cov_4.484665, whole genome shotgun sequence.
ACCESSION   DAOQOC010000284 DAOQOC010000000
VERSION     DAOQOC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18903_length_2859_cov_4.484665"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PL106_05670"
     CDS             complement(<1..724)
                     /locus_tag="PL106_05670"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32591.1"
                     /translation="MSTTSLSVTSITDLLALYRTDARVAQIAEGLQTPAARVQISGTI
                     GSAQALIAHSVIEQRGGVHVFVLTDKEEAAYFINDLEALRNVREGEGARVREKKHDDM
                     FFYPAPSRSPYDPEGHHDGERVSRTEVLEELMRVNGEWRKANGAEAHSPFANSHSPFT
                     KDLVIITYPDALIPLVMGKEDMAKNTLTVKRHEDLPIDTLEEWLHETGFVRVEFVYEP
                     GQFSIRGGIVDVFSYGSDKPYRI"
     gene            complement(820..>2859)
                     /locus_tag="PL106_05675"
     CDS             complement(820..>2859)
                     /locus_tag="PL106_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32592.1"
                     /translation="VCFVPLASVTDPRLVVTSIADACGLNDRGQRPMLEVLVDVLGAK
                     NLLIVLDNFEQVLPAAPMLVQLMEHCPSLKLLVTSRTPLHLRNEHLYPVEPLEGPNGV
                     NKRAAELLTLPAIQLFAQRAHEAAPRLVLDEPNVQAIAEICRSLDGLPLAIELAAANV
                     KYFPPVALRSRMHGALGMLTRGAVDLPERQRTMRAAIDWSFRLLQPALQAFFGRLGSF
                     HGPFSLEDADEVAGARSLKLDPLHALEDLVDHGLLTLVPQPVESSSLPHFNLLHVVRE
                     FAIEVLKERNELQEVIARHAEHYIHHLANRERPRRNEEMVAWYDTMEASFADLRGAFH
                     SALNMKDLRSAWSLIGHLGDFVVRRGHLGEALTWLQAAGVDRAADGDLPTLVPTTTVI
                     KALNSSGRLHFFAGNFGTAKRHTVLAVELARTGDDPAAVSVPWAYLGMARLCLGEAEA
                     KDDLAGCAEHARKHGHAYGEAFALTFLAEAMMARGEMDQARKTIELAQECIAKEGLYD
                     QEPNILVMLASWHLRMGETASAENVYRKSIAAYERTGMVQIKGWAHVGLATSLLFMER
                     VDEARSEYSIALEIGRRNGDRSIIRTCLLGFGFLHLSTDPGKSARLLGALSAIKNELH
                     FSGWTANQGMIDRLQVKVQAQLPPVRFEELHAEGLELPMGKAIDLALSAGRVTAS"
BASE COUNT          547 a          864 c          870 g          578 t
ORIGIN      
        1 cgatgcggta gggcttgtcg ctgccgtagc tgaacacgtc cacgatgccg ccgcggatgc
       61 tgaactgccc cggctcgtac acgaattcca cgcgcacgaa gccggtctcg tgcagccatt
      121 cctccagggt gtcgatgggg aggtcttcgt ggcgcttgac cgttagggtg ttcttcgcca
      181 tgtcctcttt gcccatcacc agggggatga gggcatcggg ataggtaatg atgacgagat
      241 ccttggtgaa tggtgaatgg gagttggcga atggagaatg tgcctccgct ccattcgctt
      301 ttcgccattc accattcacc ctcatcagct cctccagcac ctcggtgcgg ctcacgcgct
      361 cgccatcgtg gtggccttca ggatcgtacg gcgagcgtga aggtgcgggg tagaagaaca
      421 tgtcgtcatg tttcttctct cgcaccctcg caccctctcc ctctcgcaca ttcctcagcg
      481 cctccagatc attgatgaag tacgccgcct cctccttgtc ggtgagcacg aacacatgca
      541 cgccgccacg ttgctcgatc accgaatgcg cgatcaaggc ttgcgcggaa ccgatggtgc
      601 cgctgatctg cacgcgtgcg gccggggttt gcaaaccttc ggcgatctgg gctacacggg
      661 catcggtgcg gtagagggcg aggaggtccg tgatgctggt gacggaaagg gaggtggtgg
      721 acatgcttgc tggaacgccc gaaacccggt gccgcgaatt gcgggcatcg ggttcgggag
      781 cgggtgtaaa gctatgaagg tgctaccgca ggcgatggat catgatgcgg ttacacggcc
      841 agcgctcaat gcaagatcga tcgccttgcc cattggcaac tcaagacctt cggcgtgcaa
      901 ctcctcgaac cggacggggg gtagttgtgc ttgcaccttc acctgcaagc ggtcgatcat
      961 gccttggttg gctgtccagc cggagaagtg aagttcgttc ttgatcgcgg aaagggcacc
     1021 gagcagtcgc gccgacttgc ctgggtctgt ggacaggtgg aggaatccaa agcccagcag
     1081 gcaggtacga atgatcgatc ggtcgccgtt acgtcggccg atctcgagag caatgctgta
     1141 ttccgaacgg gcttcatcca cgcgttccat gaacagcaag gaggtcgcca gccctacatg
     1201 cgcccagccc ttgatctgta ccatgccggt gcgctcatac gcggctatgc tcttgcgata
     1261 cacgttctca gccgatgcgg tttctcccat gcgcaggtgc cagcttgcga gcatcaccag
     1321 gatattgggt tcctggtcgt acaatccttc tttggcaatg cactcctgtg caagttcgat
     1381 ggtctttcgc gcttggtcca tttcaccgcg cgccatcatc gcttcagcaa ggaaggtcaa
     1441 tgcaaaggct tcgccatagg cgtggccgtg cttgcgtgcg tgctcggcgc aaccggccag
     1501 gtcatccttc gcttccgctt cgcccaggca taatcgtgcc atacccaggt aggcccaagg
     1561 cacgcttacc gcagcaggat cgtcgcccgt gcgggccagt tccacggcca gcaccgtgtg
     1621 ccgcttggcc gtgccgaaat tccctgcgaa gaagtgcaga cggccgctcg aattgagcgc
     1681 cttgatgaca gtagttgttg gcaccagtgt ggggagatcg ccatcggccg cgcggtccac
     1741 gcccgccgcc tgcagccagg tgagcgcctc accaaggtgg cctctgcgga ccacgaaatc
     1801 acccaggtgg ccgatcaagc tccacgcgct gcgcagatcc ttcatgttga gcgccgaatg
     1861 gaatgcgcca cgcaggtcgg cgaaagaggc ttccatggta tcgtaccaag ccaccatctc
     1921 ctcattgcgc cggggtcgtt cccggttcgc gaggtgatgg atatagtgct cagcatggcg
     1981 tgcgatgact tcttgcagtt cattccgttc cttgagcacc tctatggcga actctcgaac
     2041 aacatgcaat aagttgaagt gtggtagtga gctggactca acgggttgtg gcacaagcgt
     2101 caaaagaccg tggtccacga ggtcttcgag ggcatgcaac ggatcaagct tgagcgatcg
     2161 tgccccggcc acctcatcgg cgtcctccag tgagaaagga ccgtggaacg aaccgagccg
     2221 cccgaaaaag gcctgtagcg ctggttgaag aagtcggaag ctccaatcga tcgccgcacg
     2281 catggtgcgc tgccgttcgg gcaggtccac tgctccacgc gtgagcatgc ccagcgcgcc
     2341 atgcatgcgc gagcgcaggg ccactggcgg gaagtatttc acattggcag cagccagctc
     2401 gatcgcaaga ggcagtccat caaggctcct gcagatctcc gcgatcgcct gtacattcgg
     2461 ctcatccagt accagcctgg gtgcggcctc atgcgcccgc tgcgcgaaga gttgaatggc
     2521 cgggagcgtc agcaattcgg ccgcacgctt gttcaccccg ttcgggccct cgagcggctc
     2581 caccggatag agatgctcat tgcgaaggtg cagcggtgtg cggctggtga ccagcagctt
     2641 caacgaaggg cagtgctcca tcagctggac cagcatcggc gcggcgggga gcacttgctc
     2701 gaagttgtcc agcacgatca gtaggttttt cgctccgagc acgtccacaa gcacctccaa
     2761 cattggtcgc tggccacggt cgttcaatcc acaggcatcg gcaatgctcg tgaccaccaa
     2821 tcgggggtct gtcaccgaag ccagcggcac gaagcaaac
//
LOCUS       DAOQOC010000285         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18936_length_2857_cov_4.363669, whole genome shotgun sequence.
ACCESSION   DAOQOC010000285 DAOQOC010000000
VERSION     DAOQOC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18936_length_2857_cov_4.363669"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..2221
                     /locus_tag="PL106_05680"
     CDS             623..2221
                     /locus_tag="PL106_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32593.1"
                     /translation="MRAHLLGLPILMVLAANGQELPAGVQPYVDYWDSLKTVKRSEGL
                     FVNGREWNEWRFWAPNGHLTEVADFKAGERDGHVVIYYDNDTVRHDGWIHQGKPDSTM
                     RSFYRTGRPMEQGSYRLGVKEGRWNYWYANGRPMMNEIWSDSIRLLLDAWDSTGTATV
                     VKGDGILHEYYPSGVLHQESTYKLGVMSGPYKEVWPTGGTKAEGSYLGGVRTGSWTWY
                     FANGKPEKKAMYRLGKLNGPYSIFFDNGKENTTGSFKEGLKDSTWTWYLPDGTREMLG
                     HFKADLQHGLWKYWYKNGQLSSTGLFADGKQEGEWVYYYEGGQNWKRGLWKAGAKDGL
                     WTTWYENGQPLQEGNYVAGKEEGEWKSWFQNGQQKDIGSFKGGHIDGHWRGWLPDGKE
                     DYDGFWANDAKNGNWKFWHANGRLKEEGPYTDNKKNGRWSSFSTNGIPISEGMYVNNT
                     PHGPWIYYDDHGVKMREQSFVNGDPDGKCTVYDMRGRVVQEMTYKAGRLNGTMTMYDT
                     DGRVLKTVEYENGTVKKALPPKLK"
     gene            complement(2349..2525)
                     /locus_tag="PL106_05685"
     CDS             complement(2349..2525)
                     /locus_tag="PL106_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32594.1"
                     /translation="MTKKDKIAFIRSSKRKTHVYNDLHRFSDNQLNELIREIVQGLVR
                     ESEIIANAYINGYR"
BASE COUNT          618 a          830 c          905 g          504 t
ORIGIN      
        1 gcccctgggc attgatgagc atccaccgcc ggtcgtgctc gggtccacgg tcggtggtcc
       61 gcacctcagc aacgctgaac cccccgagcg atttcaccgg gtagatgtgc aacgatgcga
      121 cggtgagggt catgtgggcg ggaacttcgt gacgctgtcc tcaatgcctg cggaatgaag
      181 gcgagtacca gaacggttcc gaaacgtacg atgtacatgg gactggccac cgcactgctg
      241 cggtccgctc ccggccagag gtcattttgt ccattcaggc accaggccaa agacgaatgt
      301 ggtcgaacag gaacatccgt attccatcca aggttgtggc gacaccggga acgggacaag
      361 gacaacgacc ggcggctccg cccgggaaca accggcccga tcagttcaag aacatcgtac
      421 cagcacgagg atcgagagtg cggctatcag cagcatcagg tcctccacac cccaaaaacg
      481 caaggccagc acgcgaaacg ttctcatcgc gggacttccg acgaggacgg agcacacagg
      541 tcacggcttc cacgcacgga tcgttgatga ctggccgttc ctgtccgtag cgcccttgtc
      601 cacgatccta ctttcgcccg tgatgcgcgc ccatctcctc ggtctcccga tcctcatggt
      661 acttgctgcc aacggccagg aactcccggc cggtgttcaa ccctacgtgg actactggga
      721 cagcctgaaa accgtcaagc ggagcgaggg cctgttcgtc aacggccgcg agtggaacga
      781 atggcgtttc tgggccccga acgggcatct gaccgaggtg gccgacttca aagccgggga
      841 gcgcgacggc cacgtggtca tctattacga caacgatacc gtccgccacg acggctggat
      901 ccaccagggc aaacccgata gcaccatgcg ctctttttat cgcacgggtc gccccatgga
      961 gcagggcagc taccgcttgg gcgtgaagga gggcaggtgg aactattggt acgccaacgg
     1021 ccgaccgatg atgaacgaga tctggtcgga cagcatccgc ctgctccttg acgcttggga
     1081 cagcaccgga acggccaccg tggtgaaagg ggatgggatc ctgcacgagt actaccccag
     1141 cggagtgttg caccaggagt cgacgtacaa gctcggcgtg atgagcggcc cgtacaagga
     1201 ggtgtggccc accggtggta ccaaggcgga aggcagctat ctgggtggtg tgcgtaccgg
     1261 aagctggacc tggtatttcg cgaatggcaa accggaaaag aaggccatgt accggctcgg
     1321 caagttgaac ggcccgtaca gcatcttctt cgacaacggg aaggagaaca cgaccggatc
     1381 cttcaaggag ggactgaagg acagcacctg gacatggtac ctgccggatg gtacccgcga
     1441 aatgctcggt catttcaaag cggacctaca gcacggcctg tggaagtact ggtacaagaa
     1501 cgggcagctc agttcaacgg gtctcttcgc cgatggcaag caggaagggg aatgggtgta
     1561 ctactatgag ggtggccaga attggaaacg cggcttatgg aaggcgggcg cgaaagacgg
     1621 cctctggacc acctggtacg agaatggtca gcctttgcag gagggcaact atgtggccgg
     1681 caaggaggaa ggcgagtgga agagctggtt ccagaacggc cagcagaagg acatcggcag
     1741 cttcaagggc ggccacatcg atgggcactg gcgtggctgg ctgccggacg gcaaggagga
     1801 ttacgatggt ttttgggcga acgatgccaa gaacgggaac tggaagttct ggcacgcgaa
     1861 cggccgattg aaagaggagg gaccgtacac ggacaacaag aagaacgggc gttggtcatc
     1921 cttcagcacc aacggcatac cgatcagcga gggcatgtac gtgaacaaca cgccccacgg
     1981 cccatggatc tactacgacg atcatggcgt gaaaatgcgc gagcagagtt tcgtgaacgg
     2041 cgatcccgac ggcaaatgca ccgtgtacga catgcgcggc cgtgtggtac aggagatgac
     2101 ctacaaggcg ggccgattga acggcaccat gaccatgtac gataccgatg gtcgtgtact
     2161 caagaccgtg gagtacgaga acggcacggt gaagaaggcg ctgccgccca agctgaagta
     2221 ggggcgttca cgagttccgg tcgcggactc cctcgcgaat tgtcggaacg taacaaggcc
     2281 ccggcttctg ccggggcccc attacctgtc gcttcagccc acctggggaa gccgacgcaa
     2341 tgcctgtttc agcggtagcc gttgatataa gcgttggcga tgatctcgct ttcccttacc
     2401 agcccctgca cgatctcgcg gatcaactca ttgagctggt tgtcgctgaa ccggtgcagg
     2461 tcgttgtata catgcgtttt gcgtttgctg ctcctgatga aggctatttt gtccttcttc
     2521 gtcatgcccc tttcaacggc aacggcccag cgttgttcca cagggcggaa gaaattcttc
     2581 ggggccgcga tcggcgcggg ctcccggtcg gaaaacacca cggcggaccc catccctgga
     2641 agcccgccgt ggcccttcac caaaaccttc ccggtgtcgc cccggagttt cgtccggggc
     2701 atccgggttc ttgcaggtcg gtccctattt ccgaccggac cgggtcgacg gccttacggg
     2761 gccgccttgc ccgggtatcg ttacgccacc gctgccgtac gggcgcggag gtcgtgcatg
     2821 agtcgcagtg catgcaggta ccggcgcagg tccccgg
//
LOCUS       DAOQOC010000286         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18978_length_2854_cov_3.506967, whole genome shotgun sequence.
ACCESSION   DAOQOC010000286 DAOQOC010000000
VERSION     DAOQOC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18978_length_2854_cov_3.506967"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PL106_05690"
     CDS             <1..936
                     /locus_tag="PL106_05690"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNA32595.1"
                     /translation="GESDGPQLDENMGIIMANETSVFGDPYRVIRIPMPPSTGGEWAP
                     DASYRTYANNLFVNGTVIVPTYRTEYDTTALRILHEALPGYRIVGIDCDNSGSNIISA
                     SGAIHCITKGIGVNDPLLIKHQRLHDTYDTQNPYEVTAWMRHKTGIAAANLFWTTDTT
                     QTWNQVAMTDIGDNDHSAAIPAQPAGTTVYYYVQGIAVSGKTQVRPIVAPDGWWSFRV
                     LDANTAVVEAQGPAIADLFPNPCTSHVSLKLENSAGALVSILDVTGRTVLSYKGTRVP
                     NEGRLLLDVSMLPTGTYALTVASSKGTATRKLMKH"
     gene            980..1342
                     /locus_tag="PL106_05695"
     CDS             980..1342
                     /locus_tag="PL106_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32596.1"
                     /translation="MNLRTLLPFALLVCANTLLAQAPNTIWSYAVGDWRNGPVVKISP
                     LFETTEAFTTPQLIAYVKKTYPAFAKVSDIDIQRFATIEEGELSRKTLKGKYHARKLE
                     VDMDETPFKPKEAPVHNE"
     gene            complement(1507..2745)
                     /locus_tag="PL106_05700"
     CDS             complement(1507..2745)
                     /locus_tag="PL106_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32597.1"
                     /translation="MDFSYYISGLGGGKLTPWNELMYVAGGQTVRRLLADGHVDPTWQ
                     ELELDPYIHILQGGDYHVFPDGRLVITGTHALYDSIRGFVGLYNLIWITADGRLDTTR
                     VHRRGNGDLDTILELADGSFMISGGLTEYEGHPVGDIIRGFADGSLDTTFHSTVDWFW
                     PFTYLPLPDGKVLCGGTMHFAGIPDTLNLVRLLPDGTLDPTFENSTDYRSSFNSSGSG
                     GVASITPYQADRYIVTGYFDSVNGEQRGGIALVDTAGHLVDDLFNGGGCGTFWNGFYT
                     VGGINGLVPAPDGSFYIHGSYHGYNDGTTNDVYQRMVSRLYGLDVGVEEEPVQAGAIL
                     SLAPNPATDLIRVNFPTGAGAVNNLEVLDITGQLVLSARPTGQSAVLNVADLRSGVYF
                     VRLITAGAVYPASRFEVLPH"
BASE COUNT          635 a          926 c          815 g          478 t
ORIGIN      
        1 ggcgagagcg atggcccgca actggatgag aacatgggca tcatcatggc caatgagacc
       61 agcgtgttcg gcgatccata ccgcgtgatc aggatcccca tgccccccag caccggcggc
      121 gaatgggccc ccgacgcctc ctaccgaaca tacgccaaca acctcttcgt gaacggcacc
      181 gtgatcgtgc ccacctaccg caccgagtac gacaccacgg cgctgcgcat cctgcacgaa
      241 gccctgcccg gataccgcat cgtagggatc gactgcgaca atagcggcag caacatcatc
      301 agtgccagcg gagccatcca ctgcatcacc aaggggatcg gggtgaatga tccgctgctg
      361 atcaagcacc agcgcctgca cgacacctac gacacacaga acccgtacga ggtgacggcc
      421 tggatgcgcc acaagaccgg catcgcggcg gcgaacctgt tctggaccac cgacaccacc
      481 cagacctgga accaggtggc catgacggac atcggcgaca acgaccacag cgccgccatc
      541 cccgcgcaac cggccggaac caccgtgtac tactacgtac aagggatcgc ggtgagtggc
      601 aagacccagg tacgtcccat cgtggcaccg gacggttggt ggagtttccg cgttctggac
      661 gcgaacaccg ccgtggttga agcccaaggc cccgccatcg ccgacctctt tcccaatccc
      721 tgcaccagcc acgtgtcgct caaactggag aacagtgctg gtgcgcttgt ttcgatcctc
      781 gatgtgacgg gccgcacggt actgtcctac aagggcactc gtgtgccgaa cgaaggtcgc
      841 ctcctgctcg acgtgtccat gctgcccacc gggacgtatg cgctaacggt agcctcatcc
      901 aagggtacgg ccacccggaa actgatgaaa cactgactac ggcacgatga tcgctgtgca
      961 gccacagtcc tccaccttca tgaacctccg cactctcctc cccttcgcac tcctggtctg
     1021 cgcgaacacc ctgctcgccc aggcccccaa caccatctgg agctatgccg tgggcgactg
     1081 gcgcaacggc cctgtggtga agatctcccc gctgttcgag accaccgagg ccttcaccac
     1141 gccacaactg atcgcctacg tgaagaagac ctatccggcc ttcgccaagg tgagcgacat
     1201 cgacatccaa cgcttcgcca ccattgagga gggcgaactc tcgcgcaaga cactcaaggg
     1261 caagtaccac gcccgcaagc tggaagtgga catggacgaa acgccattca agccgaagga
     1321 agcgccggta cacaatgagt gagggtgaga gggtgagagg gtgagagggt gttagggtgc
     1381 gagaggacga gggtgagagg gtgcgagagt gagagggtgc gagagtgaga aggtgcgagg
     1441 gtgagagggt gcgagggtgc gagggaccgc catcgcctat caaccatccc tgaatcaggg
     1501 tatcgttcag tgtggcagca cttcgaaacg cgaagcgggg tacacggccc cggcggtgat
     1561 cagccgcacg aagtacacgc cagaacgcag gtccgctacg ttgagcaccg cgctctggcc
     1621 cgtcggtcgt gccgagagga ccagttggcc ggtgatgtcg agcacttcga ggttgtttac
     1681 cgcgcccgcg ccggtgggga agttcacgcg gatgaggtcg gtggccgggt tgggggccag
     1741 ggacaggatg gcgcccgcct gcaccggctc ctcctccacc cccacatcca acccgtacaa
     1801 ccggctcacc atgcgctggt acacatcgtt ggtggtgccg tcgttgtaac cgtggtagct
     1861 gccgtggata tagaaactgc catcaggtgc cgggacaagc ccgttgatac caccgaccgt
     1921 gtagaaccca ttccagaatg tgccgcatcc gcctccgttg aacaggtcgt ccacaaggtg
     1981 cccggcagta tccaccagag cgatcccgcc acgttgttcc ccatttaccg agtcgaaata
     2041 gccggtaacg atgtacctgt ccgcctgata cggtgttatc gatgccacac caccactccc
     2101 agaactgttg aacgaactgc gatagtccgt cgaattctcg aacgtgggat ccaacgttcc
     2161 gtccggcagc aggcgcacca ggttcagcgt gtccggtatc cctgcaaaat gcatggtacc
     2221 gccgcagagc accttgccat cgggcaatgg cagataggtg aagggccaga accaatccac
     2281 agtgctgtgg aaggtggtgt ccaatgatcc atccgcaaaa ccccggatga tatcacccac
     2341 cggatgacct tcgtactcgg tgagcccgcc actgatcatg aacgagccat cggcgagttc
     2401 caggatggtg tccagatccc cattccctcg ccggtgtacc cgcgtggtgt ccaaacgccc
     2461 atcggccgtg atccagatca ggttgtacag gcccacaaag ccacggatgc tgtcgtacag
     2521 cgcgtgcgtg cccgtgatca ccaagcgccc gtccggaaac acgtgataat ccccgccttg
     2581 gaggatgtgg atgtaggggt ccaactccaa ttcttgccat gtagggtcca catggccatc
     2641 ggccaacaac cgacgaactg tttgaccacc cgccacgtac atcagttcgt tccatggtgt
     2701 caacttccca cccccaagac cgctgatgta gtatgaaaaa tccaaactcc cagccgacgt
     2761 gaaccttccc gaacttgtcc aataccctgg gtcgcccgga gagccgagtc gaccagaaaa
     2821 gaccaccctc ccgtcagaca atggaacaac ggac
//
LOCUS       DAOQOC010000287         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18981_length_2854_cov_3.163273, whole genome shotgun sequence.
ACCESSION   DAOQOC010000287 DAOQOC010000000
VERSION     DAOQOC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18981_length_2854_cov_3.163273"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="PL106_05705"
     CDS             <1..1899
                     /locus_tag="PL106_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32598.1"
                     /translation="SGSVIQAREDFDAITDPAFRSEVMKKYKDGGYINSYWGDQDFTS
                     GPVYVGVVVVLLMLLLLARAEGHARWWVPGSLLLIGALLNITSPMLAGALVIVYLITG
                     IFLWKDTLAYALFSGLLLTLLLSWGKFYMPLTDFFLDHVPGYDKFRAVTIILVIVELC
                     APVLAVLYLDRLLKEEKWNAVTQRNFLMASGAFLLLLLVMAFAAPSLFNFFSDKEKVE
                     LADGSQTYVDGLKALRLGIFTSDVWRSIGFLVVGAALVFTYGKRIIGGPVLLSGLGLL
                     ILADLWSVDKRYLNNEKRDGRYVSWEDDAQSKLPFKPQPADLAILQREQNAATEADLA
                     QALDRLKAAKRDSDAANKLVSKDEEAITRFGSLRRTTNFRVLTLQNPFNDSRVSYFHK
                     SLGGYHGAKLKRYQELISFRLGTEMAELLADLRKARTPGAMDSAFLDRPALNMLNMRY
                     LIASNDQPPLHNPHALGDGWFVDEVRTVKNADEEITALATIDPRRTALVDERFLKDLD
                     IAAAKPDSTATVALIGPWRANDMRYTVKSSSGGVVVFSEIWYGPDWHVEVDGQPATYA
                     RANYVLRAMSVPAGEHEVRFYIKSAPYSTGGKVASVSSWILVLLVLGAIGFVVRKQPE
                     GGFPAVVD"
     gene            1906..>2854
                     /locus_tag="PL106_05710"
     CDS             1906..>2854
                     /locus_tag="PL106_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HNA32599.1"
                     /translation="MRRVLIITYYWPPNGGAGVQRWLKMAKYLPQHGWQPVVYTPENP
                     EIITPDEALAKEVPNEAEIIKRPITEPYAFYKRLTGRKADEKVHLGFLNEGKKKSWRE
                     DLAVWLRGNVFIPDARVWWVDPSVKFLTTYLQAHPVDAIVSTGPPHSMHLIALGLKKR
                     LPALRWIADWRDPWTNIDFYDQLKLTAWADRKHHRQEREVVGRADVNVAVGWTMAEEL
                     KALGSRRTEVITNGYDPDDVPEPPEPVDEKYSLVHVGNLTATRDIPQLWPVLAELCRT
                     STEFARRFELRFVGPVDHVALASIDRAGLSDRVTRLGSVD"
BASE COUNT          537 a          862 c          915 g          540 t
ORIGIN      
        1 agtggttcgg tgatccaggc ccgtgaggac ttcgatgcca tcaccgatcc ggccttcagg
       61 tccgaggtga tgaaaaaata caaggacggc gggtacatca acagctattg gggcgatcag
      121 gatttcacca gcgggccggt gtacgtgggt gtggtggtcg tgttgctgat gctgctcctg
      181 ctggcccggg ccgaagggca cgcgcgctgg tgggtgcccg gttccttgct gctaattggc
      241 gccttgctga acatcacatc gcccatgctg gccggtgcgc tggtgatcgt ctatctgatc
      301 acaggtattt ttctgtggaa ggataccctc gcgtacgcgc tcttcagcgg cctcttgctc
      361 acactgctgc tcagttgggg caagttctac atgccgctca ccgacttctt cctggaccac
      421 gtgcccggct acgacaagtt ccgcgcggtc accatcatcc tggtgatcgt tgaactctgc
      481 gctcccgtcc tcgccgtgct ctacctcgac cggttgttga aggaggagaa gtggaacgcg
      541 gtaacacaac ggaacttcct catggcctcg ggcgctttcc tgctcctgct gctggtgatg
      601 gccttcgccg caccgagcct tttcaatttc ttcagcgaca aggagaaggt cgagctggcc
      661 gacggctccc agacctatgt ggacgggttg aaggccctgc gcctcggtat tttcacgagc
      721 gatgtgtggc gaagcatcgg cttcctggtg gtcggggccg ccctggtctt cacctatgga
      781 aagcgcatca tcggcggtcc ggtcctgctg tcgggcttgg gcctgttgat cctggcggat
      841 ctgtggtccg tggacaaacg ctacctgaac aacgagaagc gcgatggacg gtacgtgagt
      901 tgggaggatg atgcccagag caagctgccc ttcaagccgc aacccgcgga tctggccatc
      961 ctgcaacgcg aacagaacgc cgctaccgaa gccgacctcg cgcaagcgct ggaccggttg
     1021 aaggcggcga agcgcgacag cgacgcggcc aataagctcg tgagcaagga tgaggaggcc
     1081 atcacccggt tcggcagcct cagacgtacc accaacttcc gtgtgctcac cctgcagaac
     1141 cccttcaacg actcgcgggt cagttacttc cacaagagcc tgggcggtta ccacggtgcc
     1201 aagctgaaac gctaccagga attgatctcc ttccggctgg gcacggagat ggcggaactt
     1261 ctggccgacc tgcgcaaggc ccgcaccccc ggggccatgg acagcgcctt cctcgatcgc
     1321 ccggcactga acatgctgaa catgcgctac ctgatcgcca gtaacgacca gccgccgctg
     1381 cacaacccgc acgccctggg cgatggttgg ttcgtggatg aggtgcgcac cgtgaagaac
     1441 gccgatgagg agatcaccgc gctcgccacc atcgatcccc ggcgcacagc actggtcgat
     1501 gaacgcttct tgaaggacct ggacatcgcg gcggcgaagc cggacagcac cgccaccgta
     1561 gcgctcatag gaccttggcg cgccaacgac atgcgctata ccgtgaagag cagcagcggg
     1621 ggcgtggtcg tcttcagcga gatctggtac ggcccggact ggcacgtgga ggttgatgga
     1681 caacccgcca cgtacgcccg cgccaactat gtgctgcgcg ccatgagcgt gcccgcaggc
     1741 gagcacgagg tgcgcttcta catcaagagt gccccgtaca gcacgggcgg caaggtggca
     1801 agcgtgagtt catggatcct ggtgctcctg gtgctcggag ccatcggttt tgtggttcgc
     1861 aagcagccgg agggcggctt cccggccgtt gtggactgag gtcgcatgcg acgcgtactc
     1921 atcattacgt attactggcc gcctaacggt ggggccggtg tgcagcgctg gctgaagatg
     1981 gccaagtact tgccgcagca cggctggcaa ccggtggtgt acacgccgga gaacccggag
     2041 atcattacgc ccgatgaggc actggcaaag gaggtgccca acgaagccga gatcatcaag
     2101 cggccgatca ccgagccgta tgccttctat aaacgactga ccgggcgcaa ggccgatgag
     2161 aaggttcacc tcggcttcct gaacgaaggg aagaagaaga gctggcgcga ggacctggcc
     2221 gtgtggctgc gtggcaacgt tttcatcccc gatgcacggg tgtggtgggt ggatccttcg
     2281 gtgaagttcc tgaccacgta cctgcaagca catccggtgg atgccatcgt gagcacgggc
     2341 ccgccgcaca gcatgcacct gatcgccttg ggcttgaaga agcggttgcc tgcgctacgc
     2401 tggatcgccg actggcgtga tccgtggacc aacatcgatt tctacgatca gctcaagctc
     2461 acggcctggg ccgatcgcaa gcaccatcgt caggagcgtg aagtggtcgg tcgtgcggat
     2521 gtgaatgtgg ccgtgggctg gaccatggcc gaggagttga aagcactggg gtcgcggcgt
     2581 acggaggtga tcaccaacgg ctacgacccc gatgatgtac cggagccccc cgagcccgtg
     2641 gacgagaagt actcgttggt gcatgtcggc aacctcacgg ccacgcgaga cattcctcaa
     2701 ctgtggcccg tgctcgcgga actgtgccgt acgagcaccg aattcgccag gcgcttcgaa
     2761 ttgcgtttcg tgggcccggt ggaccatgtc gcgctggcca gcatcgatcg cgcgggcttg
     2821 tcggaccggg tcacgcgcct tggtagtgtg gacc
//
LOCUS       DAOQOC010000288         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_18994_length_2853_cov_3.063259, whole genome shotgun sequence.
ACCESSION   DAOQOC010000288 DAOQOC010000000
VERSION     DAOQOC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18994_length_2853_cov_3.063259"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..814)
                     /locus_tag="PL106_05715"
     CDS             complement(143..814)
                     /locus_tag="PL106_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32600.1"
                     /translation="MKTVRTAFGKNLPWMMTVLVLLLQGCLTIEEHYVFKKDGSGTLE
                     YVFDASEMGEMLKGFPGSKDDKDGMKENDLSDKIGALKAIPGITKVKHKKEDDGWVQR
                     MSLRFANINALNAALNELDADSTSADHTYFKWEGNTLVRTTGHETGDLATGMGGSGDS
                     TTAILKEMHYKFDMKFADELGEVKAADGMVKEMDGTKHVKLSTDWSMIKDDPAALDLR
                     ITLKK"
     gene            complement(911..>2853)
                     /locus_tag="PL106_05720"
     CDS             complement(911..>2853)
                     /locus_tag="PL106_05720"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32601.1"
                     /translation="YPNFIVNGNPWPSVRMYDETVAGGSNAGLIGVSNTAEPLTPGRG
                     FTAWSGATLTSTPAFTIDVRGVPVIANTPFSIPLSYTSTGNPGADGLNLVGNPLPSPI
                     DFTDVTLTNVASNYYIYDPGASANVGWDEVTQIGTGGCNGNIQSSQGFWLTATAASPS
                     ATLDESAKVLEPINGGIFSDVQDHRPMVRLFLRDQDTSFTDEALVHFISGEPSMDARD
                     MRKLVLEDAPNVISTVNGSEDLMINAMGELNDAIDIPVKVRVATSGDHSISFGSSAAI
                     GGARCLVLEDLLTGNTVAVEDGSSMNFFIDASAPVDPPRFVLHVGRAVESSVVDVACA
                     GNATGTISATGPGDGAWTWQLITPAEDMIQQGPVEGEVVFSDLPAGEYHLYVDGATGC
                     GALSQDLHIDEPGSLAGSTTVTAASCPGAGNGGADLTVMGGTAPYSFTWTDGSTTEDL
                     QNVQAGEYAVTITDGNNCQAEVSGIIVSAGAGPEASFEVSEAPLPMEDVFFFNSGDQG
                     LAYTWDFGDGATSTEVEPVHQFATAGVYTITLTATDGTCSDVFAQDLLVGSTGMIAAA
                     NSAMIAWSEGQEFVVQWHLEGVGGILAEVLDASGRSLAQHTAQGGMGRITISTQELPA
                     GVYLLRVKAGAEYRTFKLPVGR"
BASE COUNT          520 a          922 c          818 g          593 t
ORIGIN      
        1 agcacctgct cctccttgtc gtgcccgcgc atcatcacgc gcatcacgtg caacacatac
       61 ggcttgccga acctgtcgac ctgacccttg tgcaccttcg ccgcaaggcg gatggcgctc
      121 tccagcagat gatgctccac tcctacttct tcaacgtgat ccgcaggtcc aacgccgcgg
      181 gatcgtcctt gatcatggac cagtcggtgc tcagtttcac gtgcttggtc ccgtccattt
      241 ccttcaccat accatcggcc gccttcacct cgccgagttc atcggcgaat ttcatgtcga
      301 acttgtagtg catctctttg agtatcgccg tggtcgaatc acccgagcca cccatgcccg
      361 tggccaggtc gccggtctcg tgacccgtgg tgcgcaccag ggtattgcct tcccacttga
      421 aataggtgtg gtcggccgag gtactgtcag catccaactc attcagtgcg gcattcaacg
      481 cgttgatgtt ggcgaaccgg aggctcatgc gctgcaccca gccgtcatcc tcctttttat
      541 gcttcacttt ggtgataccc ggaatggcct tcaacgctcc gatcttgtca ctcaagtcgt
      601 tctccttcat gccgtccttg tcgtccttgc tccccgggaa ccccttcagc atctcgccca
      661 tttcactcgc atcgaacaca tactccaatg tgcctgagcc gtccttcttg aagacgtagt
      721 gctcctcgat ggtgaggcac ccttgcagca ggaggacgag caccgtcatc atccaaggaa
      781 ggttcttgcc gaaagcggtc cgtacggttt tcatggtggt ggggatccag cgcgaaaagt
      841 acaccgaagg gaaagacaga aggccccgtt tccggagcct tctgctgttc tggtctcttg
      901 atcgggagga tcagcgcccg accggcagct tgaacgtgcg gtattcagcg cccgccttga
      961 cccggaggag atagactccg gcgggcagtt cctgcgtgct gatcgtgatc cgtcccatgc
     1021 cgccctgtgc cgtgtgctgg gccaaggaac gacctgaagc atcgagtact tcggccagaa
     1081 tgccgcccac gccttccagg tgccactgca ccacgaattc ctggccttca ctccatgcga
     1141 tcatggccga gttcgcggcc gcgatcatac ccgtgctgcc aacgagcaga tcctgcgcga
     1201 acacgtccga gcaggttcca tccgtggccg tgagggttat ggtgtacaca ccggccgtgg
     1261 cgaattgatg caccggctcc acctccgtgc tggtggcgcc atcgccgaag tcccatgtgt
     1321 acgccaggcc ctgatcgccg ctattgaaga agaacacatc ctccatgggc aggggtgcct
     1381 cgctcacttc gaaggaggcc tcagggcccg caccagcgct cacgatgatg ccgctcacct
     1441 cggcctggca gttgttgcca tccgtgatgg tcaccgcgta ctcaccagcc tgcacgttct
     1501 gcaggtcctc ggtggtggaa ccatcggtcc atgtgaagga atacggggcg gtccctccca
     1561 tcacggtcag atccgcgcct ccgttcccgg caccggggca ggaagcggcg gtcacggtag
     1621 tggaacccgc cagcgatccg ggctcatcga tgtgcagatc ctgtgacaga gcgccgcagc
     1681 ccgtggcgcc atccacatac aaatgatact cacctgcggg caggtcgctg aacacgacct
     1741 ccccttccac cggaccctgc tggatcatgt cctcagccgg tgtgatgagt tgccaggtcc
     1801 atgccccatc gccgggaccg gtcgcactga tcgtaccggt agcattcccc gcgcaggcca
     1861 catccaccac gctgctttcc accgcgcggc caacatgcaa tacgaagcgg ggcggatcca
     1921 ctggtgccga tgcatcgatg aagaagttca tactggagcc atcctccacg gccaccgtat
     1981 tgccggtgag caggtcttcg agaaccaggc aacgcgctcc tccgatggct gcggaggacc
     2041 cgaacgagat gctgtgatca ccactggtgg ccacccgcac cttcacggga atgtcgatcg
     2101 cgtcgttcaa ctcgcccatg gcattgatca tcagatcctc gctaccgttg accgtggaaa
     2161 tgacgttcgg tgcatcctcc agcacgagct tgcgcatatc gcgcgcatcc atgctgggct
     2221 cgccgctgat gaaatgcacc agggcctcat cggtgaagct cgtatcctga tcgcgcagga
     2281 aaagacgcac catcgggcgg tggtcctgca cgtcgctgaa gatgccgccg ttgatcggtt
     2341 ccagcacctt ggcgctctca tccaaggtgg ccgaggggga ggctgcagtg gccgtgagcc
     2401 agaagccctg gctgctctgg atgttgccat tacatccgcc ggttccgatc tgggtcacct
     2461 cgtcccagcc cacgttggca ctggctcccg gatcgtagat gtagtagtta ctggccacgt
     2521 tggtgagcgt gacgtccgtg aagtcgatgg gcgaaggcaa cggattgccc acgaggttca
     2581 ggccatcggc cccagggtta ccagtgctgg tgtagctgag cggaatggag aacggtgtgt
     2641 tggcgatcac gggcacacca cgcacatcga tcgtgaaggc gggcgtactc gtgagggtag
     2701 caccactcca tgcggtaaag ccgcgcccgg gggtgagggg ttccgccgtg ttgctcacgc
     2761 cgatcaagcc agcgttgctt ccgcctgcaa cggtttcgtc gtacatgcgt acgctgggcc
     2821 acgggttgcc gttcacaatg aaattcgggt agt
//
LOCUS       DAOQOC010000289         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19001_length_2852_cov_3.589203, whole genome shotgun sequence.
ACCESSION   DAOQOC010000289 DAOQOC010000000
VERSION     DAOQOC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19001_length_2852_cov_3.589203"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1061
                     /gene="yaaA"
                     /locus_tag="PL106_05725"
     CDS             282..1061
                     /gene="yaaA"
                     /locus_tag="PL106_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNA32602.1"
                     /translation="MLILLSPAKDLAKETPVIAETTQPVLLEHAVPLATKLKTFSAKK
                     LSTLMDLSPALGELNHARYQRWGPPFTAKNSRPAVFAFNGEAYRGLDARSLDRDDLRF
                     AQHHVRILSGLYGVLRPLDLIQDHRLMMGTALKHGRARNLYAWWGDRISDVLAADLEA
                     SGDRTIINLASSEYAKAVDFKRLGARVITPVFKDQAPGGYRMVMVFAKHQRGAMTRHI
                     IRHRILEAEKVKAYQGDGYRFEPAESEADTWVFLRDGAPRA"
     gene            1096..2229
                     /locus_tag="PL106_05730"
     CDS             1096..2229
                     /locus_tag="PL106_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNA32603.1"
                     /translation="MNIHLRRLSVLNFRNHREAALDLGPQVNCITGPNGIGKTNLLDA
                     VHYLGLCKSCFEPQDGLNILHGEDHFMLKGDLVVADGTDTLVCSVRKGARKVFTRNRR
                     EYERLADHVGRMPVVIITPYDGHLVLDGSEVRRRFLDGLIAQFDRAYLDSLVRYNRAL
                     AQRNTLLKRMAMDGRGDRDTTTPWEEQLVTHGEAVHAVRRTFTDELVPMLADHYAGIT
                     SGPERVELSYHSELGERPMRAILDERWPRDLEAGHTTGGIHKDDLHFTIGGRPLKRYG
                     SQGQQKTYLIALKLAQFDLTAQRSGLRPILLLDDIFDKIDPQRMRHLLRLLGGGRFGQ
                     TIIPDTDAARLRTALEGLPLDTRFFELDHAGTQEQRVVVEGGT"
     gene            2183..2479
                     /locus_tag="PL106_05735"
     CDS             2183..2479
                     /locus_tag="PL106_05735"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNA32604.1"
                     /translation="MPVRKSNELSLKEALEGLIHDFGLRGRLDEQAVRAVWPEVAGAM
                     IARHTLKLDLRNGKLTVKVDSAPLREELHYMRSAITELLNRKLERQVVVEIRIA"
     gene            complement(2519..>2852)
                     /locus_tag="PL106_05740"
     CDS             complement(2519..>2852)
                     /locus_tag="PL106_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNA32605.1"
                     /translation="NGFRVISLKYTRLSPQEAGAFYAVHKERPFYGELVDYMASGPIY
                     AAILEKANAVEDFRKLIGATDPANAEDGTIRKRFAESKAKNAVHGSDSDENAAIEGAY
                     FFTGREQH"
BASE COUNT          556 a          905 c          899 g          492 t
ORIGIN      
        1 gtgctggcca gtcccgccga ggtctgctac accgactttc tccagaagta cccgggtctc
       61 agcgaacgca tcccccagca actgatcgcc agttacgtgg gcatcacccc cgagttcctc
      121 agcaaactgc ggggacggat gagccgaaga ggatagggac aggacagggt gaaatgaccg
      181 atcaaacagg tgtttacgcg tatgacccgg atgtgctgga gcagcgtcat gcccgctcgc
      241 agggccagtt gtccatcatc gccggataac ttgccctccg catgctgatc ctcctctctc
      301 cggccaagga cctggccaag gaaacaccgg tgatcgccga aaccacgcaa cccgtgcttt
      361 tggagcatgc ggtgccgttg gccaccaagc tcaagacctt cagcgcaaag aaactgtcga
      421 cgctcatgga cctgagtccc gcactggggg aactgaacca tgcgcgctac cagcgttggg
      481 gaccgccctt cacggccaag aactcccgcc cggcggtctt cgctttcaat ggcgaggcgt
      541 accgcggcct cgatgcgcgc tcgctggacc gcgatgatct ccgcttcgca caacaccacg
      601 tgcgcatctt gagcggactg tacggtgtgc tgcgtccgct cgacctgatc caggatcatc
      661 ggctgatgat gggcaccgca ctgaagcatg gtcgtgccag gaacctgtac gcctggtggg
      721 gcgatcgcat cagcgacgtg ctggccgcgg accttgaagc ttcgggcgac cgcaccatca
      781 tcaacctggc cagcagcgaa tacgccaagg ccgttgactt caagcggttg ggagcacggg
      841 tgatcacgcc ggtgttcaag gatcaggccc cgggcggtta caggatggtg atggtcttcg
      901 ccaagcacca gcgcggggcc atgacgcggc acatcatccg gcatcgcatc ctggaggcgg
      961 agaaggtgaa ggcctaccaa ggcgacggct atcggttcga accggcggag agcgaggcgg
     1021 acacctgggt cttcctgcgc gatggcgcgc cccgtgcttg aaggaccggc agcgggatac
     1081 ctttgccctc cacgcatgaa catccacctg cgccgccttt ccgtactgaa cttccggaac
     1141 catcgggagg ccgcactgga cctgggcccg caggtgaact gcatcacggg gcccaacggc
     1201 atcggaaaga cgaacctgtt ggatgcggtg cactacctcg gcctgtgcaa gagctgcttc
     1261 gagccgcagg acggtctgaa catactgcac ggggaggatc acttcatgct gaagggcgac
     1321 ctggtcgttg cggacgggac ggacacgctg gtgtgcagcg tgcgcaaggg cgcgcgcaag
     1381 gtgttcacgc gcaaccgcag ggaatacgaa cgactggccg atcatgtggg ccggatgccc
     1441 gtggtgatca tcaccccgta cgacggccac ttggtactgg atggcagtga ggttcggcgg
     1501 cgctttctcg atggcctcat cgcacagttc gaccgtgcct acctcgattc attggtgcgg
     1561 tacaaccgcg cactggccca acgcaacacg ctgttgaagc ggatggccat ggatggccgt
     1621 ggcgatcggg acaccaccac gccgtgggag gagcagctcg tcacgcatgg cgaggcggta
     1681 catgccgtgc gccggacctt cacggacgaa ctggtcccga tgttggcgga ccactacgcg
     1741 ggcatcacca gcggacccga gcgggtggaa ctttcctacc acagcgaact gggggagcgc
     1801 cccatgcggg cgatcctcga tgagcgctgg ccgcgcgacc tggaagccgg gcacaccacg
     1861 ggcgggatcc acaaggacga cctgcacttc acgatcggag ggcgccccct gaagcggtac
     1921 ggcagccagg gccagcagaa aacatacctg atcgccctga agctcgccca gttcgacctg
     1981 acggcgcagc gcagcggact gcggcccatc ctccttctgg acgacatctt cgacaagatc
     2041 gatccgcaac gcatgcgaca tctgctcagg ctcctcggcg gcggtcgctt cgggcagacg
     2101 atcatccccg ataccgatgc ggcacgcctg cgcacagcgc tggagggcct gccactggac
     2161 acccgtttct ttgaactcga ccatgccggt acgcaagagc aacgagttgt cgttgaagga
     2221 ggcacttgag ggcctcatcc atgacttcgg cctgcgcggc aggttggatg agcaggccgt
     2281 gcgtgcggtc tggcccgagg tggccggtgc catgatcgcc aggcataccc tgaagctgga
     2341 cctgcgtaac ggcaagctca cggtgaaagt ggacagcgcc ccgctgcgcg aggaactcca
     2401 ctacatgcgc tcggcgataa ccgaactgct caaccggaag ttggaacggc aggtcgtggt
     2461 ggagatccgg atcgcctgac ccaacaccac cacatgcacg ccgctctgat cttgcggatc
     2521 agtgttgttc gcggccagtg aagaagtacg cgccctcgat ggcggcattc tcatcactgt
     2581 cgctgccatg caccgcattc ttcgctttgc tctcggcgaa gcgcttacgg atggtgccgt
     2641 cctcggcatt ggctggatcg gtggccccga tcagcttgcg gaaatcctcc accgcattgg
     2701 ccttctcaag gatcgccgcg tagatggggc cagaggccat gtagtccacc agctcgccat
     2761 agaacgggcg ctccttgtgt accgcataga acgccccggc ttcctgcggg gagaggcgtg
     2821 tgtatttcaa ggagatcacg cggaatccgt tc
//
LOCUS       DAOQOC010000290         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19009_length_2852_cov_3.081873, whole genome shotgun sequence.
ACCESSION   DAOQOC010000290 DAOQOC010000000
VERSION     DAOQOC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19009_length_2852_cov_3.081873"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PL106_05745"
     CDS             <1..773
                     /locus_tag="PL106_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNA32606.1"
                     /translation="NIHTDKGPVKAVFGWPAIHVRTGKNDPVPSMDNIFLDCGCSSKE
                     EVEKLGVHVGCVAVFEDGFMTLNERHYVGRALDNRMGGFMIAQVARLLKENKVKLPFG
                     LYITNSVQEEVGLRGAEMIVERIRPDLAIVTDVTHDTQTPMMNKIQSGDVACGAGPVL
                     SYGPAVHNTVLKRIIEAAEKEKIKFQRAAVSRATGTDTDAFAYGAAGVPSALISLPLR
                     YMHTTVESVHRDDVEDVIRLIHASLRNLKPGEDLRYLR"
     gene            825..1463
                     /locus_tag="PL106_05750"
     CDS             825..1463
                     /locus_tag="PL106_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32607.1"
                     /translation="MKFELDENQRGAFHFLLLGVLVVGLPALIIHLVDESQTAVVTTD
                     IHRLQVFRNGYLLSDPGLVASASSTRGERLVAGVLLGLAIGTVVAALCWLLRLRPGHW
                     MAGRWSGGIAMAYFIPVALLLPANMCAPSRGLLVRTGSRSVPRTDLPLPFTSHTDTIL
                     LGPEQLALSRGNGAWQVRLRSDDREILIASSYADSALVAAGVDQLRRIINPE"
     gene            1460..2593
                     /locus_tag="PL106_05755"
     CDS             1460..2593
                     /locus_tag="PL106_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNA32608.1"
                     /translation="MTDRPFTLDRLVRSVGTLLVLAALLWLLWYLRAVLVPFFIALLL
                     AYLLEPLVRLVRRRVKNHTVAVLIPLTGIFLLTVSLLLVIIPQITAETRHFARLMTEQ
                     MPAWQAEAEHLPWVRRMLEQLSSVDVQSYLTGESLARLAQKVLPGFWQGINNVFGWLL
                     GLAGAITTLIYLVFMLIDQEDVSAHWRNMVPEKYRDLVAGLATDLGEAMEVHFRGQMK
                     IALILSVIYTIGFWLVGLPLSVVLGLLAGMLSLIPYFALLSVPPVLLCALLAALSNGG
                     YFWPPVIGALAVYAVAQTIEGLVLVPRIQGKSAGLRPEYVLLALSVWGALLGVVGMII
                     ALPLTSVLISWYRRFILGQAEERPTGPAPAETGRPSPGGTGAA"
     gene            2665..>2852
                     /locus_tag="PL106_05760"
     CDS             2665..>2852
                     /locus_tag="PL106_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNA32609.1"
                     /translation="MRIRFIKAKDTWPLRQLVLRPGQPIEECDFPNDRNPDSFHLGAE
                     ERSVIVVVASFYGERHDAL"
BASE COUNT          475 a          917 c          915 g          545 t
ORIGIN      
        1 tcaacatcca caccgacaag ggccccgtga aggccgtgtt cggctggccc gccatccatg
       61 tgcgcaccgg caagaacgac cccgtgccca gcatggacaa catcttcctg gactgcgggt
      121 gcagcagcaa ggaggaagtg gagaaactcg gcgtgcatgt gggttgtgtg gcggtgttcg
      181 aggacggctt catgacgctg aacgaacggc actatgtggg ccgcgcactg gacaaccgga
      241 tgggcggctt catgatcgcg caggtggcgc ggttgctgaa ggagaacaag gtgaaactgc
      301 ccttcggcct gtacatcacc aacagcgtgc aggaagaggt gggcctgcgc ggtgccgaaa
      361 tgatcgtgga gcggatccgg cccgacctgg ccatcgtgac ggacgttacg cacgacacgc
      421 agacccccat gatgaacaag atccagagcg gcgatgtggc ctgtggtgcg ggccctgtgc
      481 tcagctacgg tcctgcggtc cacaacacgg tgctcaaacg catcatcgaa gcggccgaga
      541 aggagaagat caaattccag cgcgccgcgg tgagccgcgc caccggaacc gacacggatg
      601 cgttcgcata cggtgccgcc ggcgtgccca gcgcactcat cagcctgccg ctgcgttaca
      661 tgcacaccac ggtggaaagc gttcatcgcg acgatgtgga ggatgtgatc cggctgatcc
      721 acgcctccct tcgcaacctg aagccggggg aggatctgcg gtacctccgg tagggtacgg
      781 gtcgcactgc acactgatcg gttccatgca ccgatcttcg gcgcgtgaaa ttcgaactgg
      841 acgagaacca acggggcgcg ttccacttcc tgcttctcgg tgtgctcgtg gtgggcctgc
      901 ccgcgctgat catccacctg gtcgacgagt cgcaaacggc ggtggtcacc accgatatcc
      961 accggctgca ggtgttcagg aacggatacc tgctcagcga tccagggctg gtcgcatccg
     1021 cttcatcaac gcgtggcgag cgcctcgtcg cgggggtgct tctgggcctg gccatcggta
     1081 ccgtggtggc ggcactgtgt tggctgctcc gactcaggcc agggcactgg atggcgggtc
     1141 gctggtccgg gggcatcgcc atggcctatt tcatcccggt cgcactgctc ctccccgcga
     1201 acatgtgcgc accatcgcgt ggcctccttg tacgtaccgg ttcacggagc gtaccccgca
     1261 ccgatcttcc cttgccgttc acctcgcaca cggataccat attgctggga ccggaacagc
     1321 tggccctgtc gcgtggcaac ggggcctggc aggtacggct gcgctcggat gaccgggaga
     1381 tcctgatcgc ctcgtcgtat gcggacagtg ccctcgttgc ggccggggtg gaccaattgc
     1441 gccgtatcat caaccccgaa tgaccgatcg ccccttcaca ctggaccgcc tggtccgatc
     1501 ggtgggcacc ctgctcgtac tggccgctct gctatggctg ctctggtacc tgcgtgcggt
     1561 gctggtgccc tttttcatcg cgctattgct ggcctacctg ctggagccgc tcgtgcgcct
     1621 ggtgcgccga cgggtgaaga accatacggt tgcggtgctg atccccctga caggtatttt
     1681 tcttctcacc gtttcgctgc tcctggtgat cattccgcag atcacggccg aaacccgcca
     1741 cttcgcccgc ttgatgaccg aacagatgcc cgcatggcag gccgaggcag agcacctgcc
     1801 ctgggtccgg cgcatgctgg aacaactctc gtccgtggat gtccagagct acctcacggg
     1861 cgaaagtctg gcccgtctgg cgcagaaggt gctgcccggt ttctggcagg ggatcaacaa
     1921 cgtgttcggc tggctgctcg gcctcgcggg agccatcacc acactgatct acctggtctt
     1981 catgctcatt gaccaggagg acgtgagtgc ccattggcga aacatggtgc ccgaaaaata
     2041 ccgggatctg gtcgccggcc tcgccaccga cctgggcgaa gccatggagg tccatttccg
     2101 ggggcagatg aagatcgcac ttatcctttc ggtcatctac accatcggat tctggctggt
     2161 cggcctgcct ctgtcggtgg tgctcggact gctggcgggc atgctgagcc tgatccccta
     2221 tttcgccctc ttgagcgtgc ccccggtcct gctctgcgcg ctgctggccg cactttccaa
     2281 cggtggctac ttctggcccc ccgtgatcgg ggcgctggcc gtttacgccg tggcacaaac
     2341 gatcgaagga ctggtgctgg tgccccgcat ccagggcaag agtgccgggc tgcggcccga
     2401 gtacgtgctg ctcgccctct cggtgtgggg cgccctgctg ggggtggtgg gcatgatcat
     2461 cgccctgcca ctcaccagcg tactgatcag ctggtaccgg cgcttcatcc tgggccaggc
     2521 ggaggagcgg ccaacgggac cagcccccgc ggagaccggg aggccatccc ccggggggac
     2581 tggcgcggcg tagaggatct cccgaacttg cgggactttt ccgatggtca tgccccatcg
     2641 gtgtgaagca gcggtcctct gcccatgcgc atccggttca tcaaagcgaa ggacacctgg
     2701 ccgttgcggc aactggtgct gcggccgggt cagcccatcg aggagtgcga cttccccaac
     2761 gaccggaacc cggacagctt ccacctgggt gccgaggaac ggtccgtcat cgtggtggtg
     2821 gcctccttct acggggagcg ccacgatgcc ct
//
LOCUS       DAOQOC010000291         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19022_length_2851_cov_2.879471, whole genome shotgun sequence.
ACCESSION   DAOQOC010000291 DAOQOC010000000
VERSION     DAOQOC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19022_length_2851_cov_2.879471"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PL106_05765"
     CDS             <1..856
                     /locus_tag="PL106_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32610.1"
                     /translation="GDSLLRHNKPQKALLKFNEALELDPTATNYAARARAWIMLDRMD
                     RVLLDAEQALKLDSTHAEANHQRALYAFRSDDFRLADRLTTRALNNGARGRWRDKLLI
                     LRGESRAELKRASEAIADLKAGLGDRTDDLAALKTLARLLDGTSDHAASLAVLEKLCV
                     VEPGDIGNWTNRGFELASLGRHQEALAIYAKALAMDKDEPTALSNRAWSLMELGRKEE
                     ALSDVQRSLRSYPANAFALRTRALLRIGMGQRDKACEDLHLARVIGGVADVPQLIAKH
                     CAGLEPKR"
     gene            complement(865..>2851)
                     /locus_tag="PL106_05770"
     CDS             complement(865..>2851)
                     /locus_tag="PL106_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNA32611.1"
                     /translation="YQAPDWITSHSLFIDEHGKLFLNGSNRGNGGCIIYDLTQDPEAP
                     VEVGEYDQWYVHDCYARGDTLYAAHIYDGFFSIVDVSDPSDPQLLGTMNTPSLFTHNC
                     WLDDSGQYLFTTDEKPNSFLASYDVSDPTDVQYLDKLQTDPGSNAIIHNTYWLNGYVV
                     QSYYTEGVSIYDVHDPTNIVEVGRFDTSPLTGDGFDGAWGVYPFLPSGRLLVSDIQEG
                     LFILEPTYVQACLLQGLISDANTSAPVNGATVTLDGTTASDLTDGFDGQYATGWATAG
                     TYSVTVSAPGYVGQTVPGVVLTNGSVTTQDVELVPLTPFNVAGTVIEAGTGTPVAAAT
                     VVLSNETYQFSATTDANGQFSIPNAFAGDYQGLAGHWGHHTVCTEVPGLAQGSPAITF
                     ELPAGYADDFALDLGWTATSEASSGDWVREAPVGTSFFQQAVAPGADMTDDCGELAYV
                     TGNGGGEAGDDDVDQGTVELASPAFDATLTTDPWIRYYRWWYTGGGNSDSNDELVIKL
                     DNGGDLFTVETLDLDAPDQAAWVMHQFRIADLTTPTANMRLIVSTGDDDPGHLVEAGL
                     DRFEVLPESPFLTVPETAPQDMLLFPDPSDGACTLLAPGISGRVRVLDARGRQVLAPV
                     RFNGGSLALRIDAGAGLYLVRIDQDNGAVRTLRMVVR"
BASE COUNT          548 a          864 c          936 g          503 t
ORIGIN      
        1 aggggacagc ctgctccgcc acaacaagcc gcagaaagcc ttgctgaaat tcaacgaggc
       61 ccttgagttg gaccccacgg ccaccaacta tgcggcccgg gcccgtgcgt ggatcatgct
      121 ggaccggatg gaccgtgtgc tcctggatgc ggagcaggcc ctcaaactgg acagcaccca
      181 cgctgaggcc aatcaccaac gggccctgta cgcgttccgg agcgacgact tccgtctggc
      241 cgatcgcctg accactcggg cgctgaacaa cggggccagg ggacgctggc gagataaatt
      301 actgatctta aggggtgaat ccagggcgga actcaagcgt gcgagcgagg ccatcgccga
      361 cctgaaagcc gggctcggcg accgtaccga tgatctggcc gccttgaaaa ccctcgcccg
      421 attgctcgat gggacgtcgg atcatgcggc cagtcttgcg gtactggaga aattgtgcgt
      481 ggtagaaccg ggcgacatcg gcaactggac caaccggggt ttcgaactcg cctcgcttgg
      541 tcggcaccag gaagcactcg cgatctacgc gaaagccttg gccatggaca aggacgaacc
      601 aacagcattg agcaaccgtg cctggtcgtt gatggaactt gggcgcaaag aagaagccct
      661 gagcgacgtg caacgcagct tgcgatccta cccggcgaac gcgttcgccc tgcgcacgcg
      721 ggcactgctg cgtatcggta tgggacagcg cgacaaggcc tgcgaggatc tgcacctggc
      781 ccgtgtgatc ggcggcgtgg ccgatgttcc ccagctgatc gcgaagcatt gcgcgggcct
      841 ggagcccaag cggtgagctg gatctcagcg cacgaccatg cgcagcgtgc gcaccgcacc
      901 attgtcctgg tcgatgcgta ccaggtagag cccggcaccg gcatcgatcc gcaaggcaag
      961 ggatccaccg ttgaagcgca ccggggccag tacttggcgg cctcgcgcat ccagcacgcg
     1021 gacccggcca ctgatccccg gcgccagcaa cgtacacgcg ccatcgctcg ggtcagggaa
     1081 cagcaacatg tcctgcggcg cggtttcggg caccgtcaag aaaggcgatt ccggcagcac
     1141 ctcgaagcgg tcgaggccgg cttccaccag atggccgggg tcatcatccc ccgtggagac
     1201 gatcaagcgc atgttcgccg tgggggtggt gaggtcggcg atacggaact ggtgcatcac
     1261 ccaagcggcc tggtcgggcg cgtcgaggtc aagtgtttcc acggtgaaaa gatccccacc
     1321 gttgtccagc ttgatcacga gttcatcgtt gctgtcgctg ttgccgccac cggtgtacca
     1381 ccagcggtag tagcggatcc aagggtcggt ggtcagcgtg gcgtcgaacg cgggcgaagc
     1441 gagttccaca gtgccttggt ccacatcgtc gtcaccggcc tcgcctccac cattgccggt
     1501 aacgtaggcc agctcgccgc aatcgtcggt catatcggcc cccggggcca cggcctgttg
     1561 gaaaaagctg gtgccaactg gagcttcccg cacccaatcg ccactggagg cctcgctggt
     1621 ggccgtccag cccaggtcga gtgcgaaatc atcggcatag cccgcaggca gttcgaacgt
     1681 gatggcgggc gatccttgtg cgagccccgg cacctcggta cacacggtgt ggtgacccca
     1741 atgcccggca agtccctggt agtcgccggc gaaggcgttg gggatgctga actgtccgtt
     1801 ggcatcggtg gtggcactga actggtaggt ctcgttggag agcaccacgg tggcggctgc
     1861 taccggagtg ccggtacccg cttcgatgac ggtgccggcc acattgaagg gcgtgagcgg
     1921 aacgagttcc acatcctgcg tggtcaccga gccgttggtg agcaccacgc ccggcacggt
     1981 ctggcccaca tagcccgggg ccgaaacagt gacgctgtag gtgccggccg tggcccaacc
     2041 ggtggcgtac tggccatcga agccgtcggt caggtccgaa gccgtggtgc catcaagggt
     2101 caccgtggca ccgttcacgg gtgcgctggt gttggcatcg gagatgagac cttgcagcag
     2161 gcaagcctgc acatacgttg gttcaaggat aaaaagaccc tcctggatat cgctcaccaa
     2221 caggcggccg ctgggcagga aggggtacac gccccaagcc ccatcgaaac catcgccggt
     2281 gagcggcgag gtatcgaagc ggcccacttc cacgatgttg gtggggtcat gcacatcgta
     2341 gatggacacg ccctcggtgt agtagctctg caccacgtac ccgttcaacc agtaggtatt
     2401 gtggatgatg gcattgctgc ccggatcggt ctgcagcttg tccaggtact gcacgtcggt
     2461 cgggtcgctc acatcgtagg aagcgaggaa gctgttgggt ttctcgtcgg tagtgaagag
     2521 atattggccg ctatcgtcga gccagcaatt gtgcgtgaac aggctcggcg tgttcatggt
     2581 tccgagcaac tggggatccg aggggtcgct cacgtccacg atgctgaaga aaccgtcgta
     2641 gatgtgggcc gcgtacagcg tgtcgcccct ggcgtagcaa tcatgcacgt accactggtc
     2701 gtattcgccc acctccacgg gtgcttccgg atcctgggtg aggtcgtaga tgatgcagcc
     2761 accgttgccg cggttcgagc cgttgaggaa gagcttgcca tgctcgtcga tgaagagcga
     2821 gtgggaggtg atccaatcgg gagcctggta c
//
LOCUS       DAOQOC010000292         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19051_length_2849_cov_3.052971, whole genome shotgun sequence.
ACCESSION   DAOQOC010000292 DAOQOC010000000
VERSION     DAOQOC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19051_length_2849_cov_3.052971"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PL106_05775"
     CDS             <1..185
                     /locus_tag="PL106_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32612.1"
                     /translation="HLIGVLSCTSQTLVLTDSQGRTLAQGDAVALDSVFSNADPGQYF
                     VRDLAASSRIQIVVHP"
     gene            269..406
                     /locus_tag="PL106_05780"
     CDS             269..406
                     /locus_tag="PL106_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32613.1"
                     /translation="MKRGTMDGASTAVTFLQSVALDAEVVTGGAYYLLAILNTRGEHQ
                     H"
     gene            complement(432..1670)
                     /locus_tag="PL106_05785"
     CDS             complement(432..1670)
                     /locus_tag="PL106_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32614.1"
                     /translation="MHPRTLLSACLVLAAAAAFAQPFPEGYARLVHERCPGADIVKVQ
                     RAAFDLIEVDYFCQGKKVELGIHNGQVVYEEHEGAPDAEVMERIRKKLDKQYSGWLLD
                     ETSLITAKDSTFIKVEVIMDGVEHNLYYTTDGKKYDPRGMMASGGWSAKDIAATLPAG
                     APYDLLTPDSSYILPPLLREVSGIAMAGPHSVYCVQDELGAVFEYDLDADAITRVLRF
                     TDVGDFEGVATHGDRITVLRSDGRIFMLDRKSGALIGDRQLSVPSLNYEGLFALPDGS
                     LLLASKEAPVVGKADLRPIHHVQGDSAFHYRELDVNAVASYLSQHWPSLIKTPARFSP
                     SAVAIHPRTGQLYVLSADDRLLAIYGDALERVTPLPATDHYKPEGLAFLPNGDLLISS
                     EGDKKGLVQASIVRFRWKGK"
     gene            complement(1688..2134)
                     /locus_tag="PL106_05790"
     CDS             complement(1688..2134)
                     /locus_tag="PL106_05790"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNA32615.1"
                     /translation="MKTLLSLAVAALTSTSLHAQHLTPAQVPAPVSTAFAQAFPKAMD
                     VEWKQKGEQYKVEFETGLLFTDHEVWYDATGKMLRHEEEISSSDLPAAVNSAIAKEFA
                     GYRVDDTERVTADGVATYVVELKMSGQPEWKVAYGADGKQLQKQAD"
     gene            complement(2235..>2849)
                     /locus_tag="PL106_05795"
     CDS             complement(2235..>2849)
                     /locus_tag="PL106_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA32616.1"
                     /translation="AFTANAAHELQTPLAIAINKLELLAEQGGTEEERMNTLGEVIAV
                     LERLTRLNKSLLLLARIENHQFPDEREVSFAAIATEVLAEFADLAEHREVTLQFTTTG
                     DLRASMDPALARTLVTNLLKNAMLHNRPGGSVSVQVDEGCFTVRNPGVTPLDAGRIFT
                     RFHKETTADGGTGLGLAIAKAISDLYGLKLEYVFEGMHMFSLRA"
BASE COUNT          491 a          841 c          935 g          582 t
ORIGIN      
        1 accacctcat cggggtgctt tcctgcactt ctcaaacgct ggtgttgacc gactctcaag
       61 gaagaacgct ggcacaaggt gatgccgttg cattggactc cgtattctcg aacgcagatc
      121 caggacaata cttcgtgcgc gacctagctg cgtcatcgag gatccagatc gtggtgcatc
      181 cgtgatcttc cgatcgcgtt ggtaacagtt gcgaggtgca tgaatgaaga gcggccgcgc
      241 gtgaaggcga ccgttccagc gcatcgaggt gaagcgcggc accatggatg gggcaagcac
      301 ggccgtcacc ttcctgcagt ccgtagctct cgatgccgaa gtggtgaccg gcggcgcgta
      361 ctacctattg gcgatactga atacgagagg cgagcatcag cattgaggag ctgggcgttc
      421 gggcgtgtcc cctacttccc cttccacctg aagcgcacaa tgctcgcctg caccaaccct
      481 ttcttatcgc cctcgctgct gatgagcagg tcgccgttgg gcaggaaggc gaggccctcg
      541 ggtttgtagt ggtcggtggc gggcaggggg gtcacgcgtt ccagtgcatc accgtagatg
      601 gcgaggagcc gatcatccgc gctgagcacg tagagctggc cggtacgcgg gtggatggcc
      661 acggcgctcg ggctgaagcg ggccggggtc tttatcagcg aaggccagtg ttggctgagg
      721 tagctggcca cggcgttgac atccagttcg cggtagtgga atgcgctgtc gccttgcacg
      781 tggtggatgg gtcgcaggtc ggctttgccc accacgggcg cttccttgct ggccagcagc
      841 aaactcccat cgggcagcgc gaagagccct tcgtagttga gcgagggcac gctgagctgg
      901 cggtcgccga tcagcgcacc gctcttgcga tcgagcatga agatccggcc gtcgctgcgc
      961 agcacggtaa tgcggtcgcc gtgcgtggcc acgccctcga agtcgcctac atcggtgaag
     1021 cgcagcacgc gggtgatggc atccgcatcg agatcgtact cgaacacggc gcccagttcg
     1081 tcctgcacgc agtacacgct gtgcgggccg gccatggcga tgcccgacac ttcgcgcagt
     1141 agcggcggca ggatgtagct gctgtcgggc gtcaacaggt cgtagggtgc gccggcgggc
     1201 agggtggctg cgatgtcctt cgcgctccaa cctccggaag ccatcatgcc gcgcggatcg
     1261 tacttcttgc cgtcggtggt gtagtagagg ttgtgttcca caccgtccat gatcacctcc
     1321 actttgatga aggtgctgtc cttggcggtg atcaggctcg tttcgtccag cagccagccg
     1381 gagtactgtt tgtccagctt cttccggatc cgctccatca cctcggcgtc gggcgctcct
     1441 tcgtgctcct cgtacaccac ttgtccgttg tggatgccga gctccacctt ctttccctgg
     1501 cagaagtagt ccacctcgat caagtcgaag gcggcacgct gcaccttcac gatgtcggcg
     1561 ccggggcagc gctcatgaac gaggcgggcg tagccttcgg ggaagggctg tgcgaaggcc
     1621 gcagcggcgg caaggaccaa gcaggcggag aggagggtgc gtggatgcat cagtgtggaa
     1681 ttcgggatca atcggcctgc ttctgcaact gcttaccgtc cgcaccgtac gccactttcc
     1741 actcgggctg gccgctcatc ttcagttcca ccacgtaggt tgccacacca tccgccgtca
     1801 cgcgctcggt atcatccacg cggtagcctg cgaattcctt ggcgatggcg ctgttcacgg
     1861 ccgcgggcag gtcactggat gagatctcct cttcatggcg aagcatcttc ccggtggcat
     1921 cgtaccacac ttcatggtcg gtgaagagca ggccggtctc gaactccacc ttgtactgtt
     1981 cgcctttctg cttccattcc acatccatgg ccttgggaaa ggcctgcgcg aaggcggtcg
     2041 atacgggcgc gggcacttgg gccggagtga ggtgctgggc gtggagggag gtgctggtga
     2101 gcgcagcaac agcgagggaa agcagggtct tcatggttcg tggttgtttg ttgatgggac
     2161 aaagggaagt ggcggatcgg gaaagaatcg ggaattgcat tcggggctgc tctgtcatgc
     2221 caggcgggtg acgctcaggc acgcaggctg aacatgtgca ttccctcgaa aacgtactcc
     2281 aacttcagtc catacagatc actgatcgcc ttggcgatgg cgagccccag accggtgccg
     2341 ccatcggcgg tggtctcctt gtggaagcgg gtgaagatgc gcccggcatc cagcggtgtg
     2401 acacctgggt tgcgcacggt gaaacagcct tcgtccactt gcacgctaac gctaccgcca
     2461 gggcggttgt gcagcatggc gttcttcagc aaattggtga ccagtgtgcg cgctaatgcg
     2521 ggatccatgg atgcccggag atcgccggtc gttgtgaatt gcaatgtcac ctcgcggtgc
     2581 tccgccaggt cggcgaactc tgcaagcact tccgtggcga tggcggcgaa ggagacttcc
     2641 cgctcatcgg ggaactggtg gttctcgatc cgcgccagca gaagcaacga cttgttgagg
     2701 cgggtgaggc gttccagcac ggcgatcact tcgcccaagg tgttcatgcg ctcctcctcg
     2761 gtcccgccct gctcggccag cagctccagc ttgttgatgg cgatggccag cggcgtctgc
     2821 aactcgtgcg cggcattggc ggtgaaggc
//
LOCUS       DAOQOC010000293         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19211_length_2836_cov_4.318590, whole genome shotgun sequence.
ACCESSION   DAOQOC010000293 DAOQOC010000000
VERSION     DAOQOC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19211_length_2836_cov_4.318590"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            575..715
                     /locus_tag="PL106_05800"
     CDS             575..715
                     /locus_tag="PL106_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32617.1"
                     /translation="MFVAYTENLDPSNDVLQLVVLRAEDGIDFLHHQPMAVPHTANDV
                     HG"
     gene            complement(1100..2551)
                     /locus_tag="PL106_05805"
     CDS             complement(1100..2551)
                     /locus_tag="PL106_05805"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNA32618.1"
                     /translation="MEIITAKTGYEAGLKDHVDREKAAIDLMNSVGQLMYAKGVELVF
                     FRNPMLDISNSQLLNLFNYAKDVVQKPIDVFQAAEVARGLLSMDLAPSKIDIGKLTWE
                     FKQNGGDLNAFLGGTLKNFIGQDKHTFKPQDVVLYGFGRIGRIAARELVKQAGKGQQL
                     RLRAIVLRSVNDEEVVKRAALLRNDSVHGAFKGAVIEDLANKAIIVNGQRVQLIAAKN
                     PEDVDYTSFGINNALVIDNTGVFTKRADLERHLKAKGVSKVLLTAPGKEIPNVVYGIN
                     HKDLDIENEKVFSAASCTTNAICPILKVMEDQLGIDRGHIETVHAYTNDQNLLDNYHK
                     KPRRGRSAAINMVITSTGAGSAVTKAIPSLKDKLTANAVRVPTPNGSLAIMSLSLKKP
                     ASRDEVNEMVRHAALNGELVNQIHYQIDPELVSSDIVGDTCCSVFDSNATIASGDGKN
                     IVVYAWYDNEFGYTKQVIRLAKHITKVRRLVYY"
     gene            complement(2621..>2836)
                     /locus_tag="PL106_05810"
     CDS             complement(2621..>2836)
                     /locus_tag="PL106_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA32619.1"
                     /translation="KEIDRALSTLTERERDVVKLFFGININHGLTLEEIGAKFDLTRE
                     RVRQIKEKAIRRLRHTSRSKLLKAYLG"
BASE COUNT          502 a          809 c          869 g          656 t
ORIGIN      
        1 gctgagcgca ccgtggccgc cgaaggcatc gtgaaccacc gcatggacct gggccacgat
       61 acgggcgcag ggctctacat ggtgaacgtg accatcgatg gcaagcgtta cacccagcgc
      121 ttggtgatcc agtaagcgca ggagcttttt gagcaggggg ccgttccgca agggacggcc
      181 ctctcgcttt ttatatgttg tgcccgggca ggggtccgat ttcccgatcg atcggactgg
      241 tgaaccggcg aagccggatc atggatcacc tatgctgccc ggggcagcac gaaccatgtt
      301 ccacgtgatg ttcctgcccg gcatgacaga ctatgcctgg gggcgtcaac ggagctccgt
      361 cattagacac ccgtcccaat tgcccagagg aatgaaaatg acctgtcacg gtgagcctgc
      421 cgaaccggtc cttgcttcgc cgtagcggct acgcaaaggc gcggtcgacg ggctcaccgt
      481 gacatgcaat aggaattcag acggaggtcg aatattccgc gtcgaccatt ttgaggtatg
      541 gtttgggccc caagcacgat cgacgtctgc ggaagtgttc gtcgcataca cggagaacct
      601 ggacccgtcg aatgatgttc tccaactggt cgtgctccgg gccgaggatg ggatcgactt
      661 ccttcaccat cagcccatgg ctgtcccgca caccgcgaat gacgtgcatg gttgatccgt
      721 ccgggctgcg gaattctcac ccgcgataga tccatgcgga tgtgttggcg accgtttgac
      781 ggatgcccat tccgcacgtg atcgcggtcc ttgagtactt cggcatccgg gccggggcga
      841 cgaatggccg gaggtatcca gcagtacggt gctgatcaag catccattga actcgtgaac
      901 cgtacacagc gggctggata ccgagcagtt catgccgttc aaggaggtca aggtgaacgc
      961 atggctggag cgtggatgtc cgatgcggag ctcttgaccc cagtggccgc ggttatcgta
     1021 catgcccggt tcgcggaata gcgggcaaga gaaagccccg ctggtgatgc ggggccttgc
     1081 tcttccggca ggaaaatgct cagtagtaca ccaaccttct caccttggtg atgtgcttgg
     1141 cgaggcggat cacttgtttg gtgtagccga actcgttgtc gtaccaggca tataccacga
     1201 tgttcttccc atccccactg gcgatggtgg cattgctgtc gaaaacgctg cagcacgtgt
     1261 cgcccacgat gtcgctgctc accagttcag gatcgatctg gtagtggatc tggttgacca
     1321 gttcgccatt gagggcggca tggcgcacca tctcgttcac ctcgtcccgc gaggccggtt
     1381 tcttcaggct caggctcatg atggcaagtg aaccattggg ggtgggcacc cgcacggcat
     1441 tggcggtcaa cttgtccttc aggctgggga tggccttggt gacggcgctg cccgcaccag
     1501 tgctggtgat caccatgttg atggccgcac tgcggcccct gcgcggtttc ttgtggtaat
     1561 tgtccagcag gttctggtca ttcgtgtagg catgtaccgt ttcgatgtgg ccacgatcga
     1621 tgccgagctg gtcctccatg accttcagga tgggacagat cgcgttggtg gtacagcttg
     1681 ccgcgctgaa caccttctcg ttctcgatgt ccaagtcctt gtggttgatg ccgtacacca
     1741 cgttggggat ctccttgccg ggtgcggtaa ggagtacttt gctcacgccc ttggccttca
     1801 gatggcgttc caggtccgca cgcttggtga acacgccggt attgtcgatc accaacgcgt
     1861 tgttgatccc aaagctcgtg tagtccacat cctccgggtt cttggcggcg atcagttgca
     1921 cgcgctggcc attgacaatg atggccttgt tcgcgaggtc ctcgatcacc gcgcccttga
     1981 atgccccgtg cacgctgtcg ttgcgcagca gggcggcacg tttcaccact tcctcgtcgt
     2041 tgacgctgcg caacacgatg gcgcgcaggc gcaactgctg ccctttcccg gcctgcttca
     2101 ccagttcacg cgcagcgatg cgcccaatgc gcccgaatcc gtacagcacc acatcctgtg
     2161 gtttgaacgt gtgcttgtcc tggccgatga aattcttcag ggtaccgccg aggaatgcgt
     2221 tcaggtcgcc accgttctgc ttgaactccc acgtgagttt gccgatatcg atcttgctgg
     2281 gagcgaggtc catggacagc agacctcgcg ccacctctgc ggcctggaag acgtcaatag
     2341 gcttctgcac aacgtccttc gcgtagttga agaggttgag cagttggctg ttgctgatgt
     2401 ccagcatcgg gttccggaag aagaccagct ccacgccttt ggcgtacatg agttggccta
     2461 ctgagttcat caggtcgatg gcggctttct cgcggtcgac atgatccttc agcccggctt
     2521 cgtaaccggt tttcgcggtg atgatctcca tgaatgggtc gttgatgggt tttatcgggt
     2581 gagttcgtgc ttgtaagcag ggtggagggg gacggtcggg tcaacccagg taggctttca
     2641 gcagtttgct tctgctcgtg tgccgcagtc ggcgaatggc cttctccttg atctggcgga
     2701 cccgttcgcg ggtgaggtcg aacttggctc cgatctcctc cagggtgagg ccgtgattga
     2761 tattgatgcc gaagaagagc ttcaccacgt cccgttcacg ttctgtgagg gtgctgaggg
     2821 cgcggtcgat ctcctt
//
LOCUS       DAOQOC010000294         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19223_length_2835_cov_3.462230, whole genome shotgun sequence.
ACCESSION   DAOQOC010000294 DAOQOC010000000
VERSION     DAOQOC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19223_length_2835_cov_3.462230"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..2624)
                     /locus_tag="PL106_05815"
     CDS             complement(9..2624)
                     /locus_tag="PL106_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol protease/hemagglutinin PrtT"
                     /protein_id="HNA32620.1"
                     /translation="MRTPHTLSRFVILGLFFLGLLGPFLGHAAQVDETVSRQVAHTFI
                     AARSGPFAGLRAEDLVLVRSVGSTSTLTGPAAVYFRIYNAGTDGFVIVAGDDAITPVL
                     GYSTEGAITGEEVPYSVAKWFDFYKGQIQEHLNAGAEIYPQVAEAWSRLLSGDAQAGG
                     DRAVNPLCQTTWDQPAPYNAQCPGGSLTGCVATAMAQVMKYHNHPAQGQGFHSYTAPN
                     YGTLSANFGSTTYNWGSMPNNVTSANSAVATLMYHCGVGVDMQYSPNLSGAWVLQSHS
                     PSTDHNTEYAMKTYFGYGTDMQGVYRDQNSLQNWNNMLKAELDASRPIIYAGFGQGGG
                     HCFVCDGYDNNSFFHFNWGWSGAYDGFFAMDALNPTGQGSGGGSGAYNSGQEALINIH
                     PATGGGGGGGNPAALVLYNYVTPSSTTIGFAQPFTVSTNILNNGTTDFNGDYAVAAFD
                     ASSNFYGFVQTYTGQQLQAGYVYNQDIVFSTNGLVTMVPGTYYLGVLFRPTGGEWTAV
                     GNNSGYTNFTQVTVVYSNDIEVYAAITPSPGNSVAQGTQLSVNLNIANAGANTFIGQY
                     GVALYDLEGNWVQDIGTIDETQGLPSGYAYNPPYLTFGPATVTVPPGTYLLACQHNPN
                     NSGWQLSGASYFQNPIFITVTGASIQPDPYEVNNTAATAYSLPVNFSGNTASTGTPGS
                     NLHVNTDMDFYKVNLAGGYDYTITARLHDSYNSGNGNTYTMDGVFSVSDNGTDWSQAV
                     DDVMAGDILFQGGGTLYFQVAPYFAGQVGTYLLSMDIQRAPSTGIAAPDLLTGISAWP
                     NPARDRLTIDMHGIPTDRAELRDAQGRLVATLFSGTSITGTYTADISGVTTGTYMLRL
                     STEKGMHTERIIVAR"
BASE COUNT          488 a          833 c          910 g          604 t
ORIGIN      
        1 ggcggtcctc atcgggctac aatgatgcgc tcggtgtgca tgcccttctc cgtggacagc
       61 cgcagcatgt aggtgcctgt ggtcacgcca ctgatgtcgg cggtgtacgt accggtgatg
      121 ctggtgccgg agaacagcgt ggcgaccagt ctgccctgcg catcgcgcag ttcggcgcga
      181 tcggtgggga tgccgtgcat atcaatggtc aaccggtcgc gtgcgggatt gggccaagcg
      241 ctgatccccg tgagcaggtc cggcgcggcg atgcctgtgc tcggggcacg ctgaatgtcc
      301 atgctcagca gataggtgcc cacctgaccg gcgaagtagg gagccacctg gaagtagagc
      361 gttccaccgc cttggaacag gatgtccccg gccatcacgt catccacggc ctgggaccag
      421 tccgttccat tgtccgagac ggagaagacc ccgtccatgg tgtaggtgtt gccattgccg
      481 ctgttgtacg agtcgtgcag ccgtgccgtg atggtgtagt cgtagccacc cgccaggttc
      541 accttgtaga agtccatatc ggtgttcacg tgcaggttgg agcccggagt gcccgtggag
      601 gccgtgtttc ccgagaagtt caccggcagc gaatacgctg tggcggcggt gttgttcacc
      661 tcgtacggat cgggctgtat gctggcaccg gtcacggtga tgaagatcgg gttctggaag
      721 tagctggccc cgctcaattg ccagccgctg ttgttgggat tgtgctggca ggccagcagg
      781 taggttccgg gaggaacggt gaccgtggcc ggaccgaatg tgaggtaggg cgggttgtag
      841 gcgtagccgc tgggcaaccc ttgggtctcg tcgatcgtgc cgatgtcctg cacccagttg
      901 ccttccagat cgtacagcgc caccccgtac tggccgatga aggtgttggc cccggcgttg
      961 gcgatgttca ggttgacgga cagttgcgtt ccctgggcca cgctgttgcc cggcgatggg
     1021 gtgatggccg cgtacacctc gatgtcgttc gaataaacta ccgttacctg ggtgaaattg
     1081 gtgtacccgc tgttgttgcc caccgcggtc cactcaccac ccgtggggcg gaacaggacg
     1141 cccaggtaat acgtgcccgg taccatggtg accaatccat tggtcgagaa cacgatgtcc
     1201 tggttgtaca cgtagcccgc ctgcaactgc tgaccagtgt acgtctgcac gaagccgtag
     1261 aagttgctcg acgcatcgaa cgcggccacg gcataatcac cgttgaagtc cgtggtcccg
     1321 ttgttcagga tgttggtgga taccgtgaac ggttgcgcga acccgatggt ggtggatgac
     1381 ggggtgacat agttgtacag caccagggcg gccgggttgc cgccgccgcc gccgccgcct
     1441 gtggccgggt ggatgttgat caacgcttcc tgcccgctgt tgtacgcgcc gcttcctcca
     1501 ccggatccct gtcctgtcgg gttcagcgca tccatcgcga agaacccatc atatgcgccg
     1561 ctccatcccc agttgaaatg gaagaagctg ttgttgtcgt acccgtcaca cacgaaacag
     1621 tgaccgcccc cctgaccgaa gccggcgtat atgatcgggc ggctggcatc cagttccgcc
     1681 ttcagcatgt tgttccaatt ctgcaacgag ttctgatccc ggtacacccc ctgcatgtcc
     1741 gtaccatagc cgaagtacgt cttcatggcg tactccgtat tgtggtcggt ggaggggctg
     1801 tgcgattgca acacccaggc accgctgagg ttggggctgt attgcatgtc cacgcccacg
     1861 ccacagtggt acatcagggt ggccacggcg ctgttcgcgc tggtcacgtt gttgggcatg
     1921 ctgccccagt tgtaggtggt actgccgaag ttggccgaaa gcgtgccata gttcggtgcg
     1981 gtatagctgt ggaagccttg gccttgcgcg gggtggttgt gatacttcat cacctgggcc
     2041 attgcggtgg ccacgcaccc ggtcaaggac ccgccggggc actgcgcgtt gtacggtgcg
     2101 ggctggtccc acgtggtctg gcacaggggg ttcaccgcgc ggtcgccccc ggcttgcgca
     2161 tcgccgctca gcaggcgcga ccacgcttcg gccacttgtg gatagatctc cgcgcccgcg
     2221 ttcaggtgct cttggatctg tcctttatag aagtcgaacc acttggccac actgtacggt
     2281 acttcttcac ctgtgatggc gccttcggtg gaatagccca gtacaggggt gatggcatca
     2341 tcaccagcga cgatcacgaa gccgtcggtc cctgcgttgt aaatgcggaa gtacacggct
     2401 gcaggtcccg tcagggtgga ggtgctgccc acgctgcgca ccagcacaag atcctcggcg
     2461 cgcagccccg cgaagggacc cgagcgcgcc gcaataaaag tgtgcgccac ctggcgcgaa
     2521 acggtttcgt ccacttgggc cgcatggccc aggaacggcc caagcaggcc caagaagaac
     2581 aggccgagga tgacgaagcg ggagagggta tggggtgttc tcatggtgaa gatgcgttct
     2641 gttgtcaaag gtggtccgat ccccaccttt ttccaaatgt cgggcattcc ccttttcggg
     2701 ggtgctgttc gtgcaccacg tccccatccc gatttttcgc catgtaccca ccggactata
     2761 ttcgcgcccc gaattttggc gcggtagctc agctggttag agcgcatgat tcataatcct
     2821 gaggtcgaga gttca
//
LOCUS       DAOQOC010000295         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19289_length_2830_cov_3.814775, whole genome shotgun sequence.
ACCESSION   DAOQOC010000295 DAOQOC010000000
VERSION     DAOQOC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19289_length_2830_cov_3.814775"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PL106_05820"
     CDS             complement(<1..1961)
                     /locus_tag="PL106_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNA32621.1"
                     /translation="MLLAKKILFGTLVIGATVLGCSTTKKAVITNVPVPDPRWAAIDS
                     LAAIGQFASALDATGTILSQAQLDGDQLTEFRAWMYKGRFQRAIGVERKEVIAAMEQR
                     ANTTVGLPLKPLLRSVIGESYWLWYQDDRWRILERTAQDEPGDDPDTWDQRTFMRKVI
                     DAYRASLDPCDTLAAMPVGDLAPLLLPERGAETILCPTVHDILARRALDIFRNSETRL
                     SEPTWRFQLDDPAFFSLFEAFAHKPLAHRDSTAREFQALQLFQRLERQHLIDARPDAL
                     VNITLDRLHFVRERSGLPQKDSLYLGALELLRSRLPNDTCEAEVITAIAQWHADQGEK
                     YQRLDPSGAWKTEKRTAADLCRTSIAKWPGSFGASNAEALLARLVMPALSVEVEEANT
                     PDVPFRAALTHTNVEQVWLRIVKDDRDEHRSRDEDGFKRLLKSPAVRAWSALLTDDGD
                     LNAHRSELPVDGLPLGNYQLIVSDAESFALDGALVLFTPFTITRLALTTREQPDRMTS
                     VLVTDRVSGAPLRSAKAELFSQRWSNGTYRDLPIGSALTDEQGIARVPVPNDLRGQYT
                     WKVSHAGDQQRISSYGWYQVEDPDRDTLRTFLFTDRAIFRPGQEVHYKGIVSVRRAGT
                     TITKASYHTSVRFVDVNGDEVAKHDVTTDA"
     gene            complement(2177..>2830)
                     /locus_tag="PL106_05825"
     CDS             complement(2177..>2830)
                     /locus_tag="PL106_05825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA32622.1"
                     /translation="IEGLERPYGHGIVNRCARLDLPRMLDAHRERWLGSGALVERTVE
                     DNDIIHEEGGVRIGEDSAPMLVRCEGPFARIDGLSQVRGDMLTVRIPGLRLKSVVHRG
                     VFLIPLGEDRYRVGSTFEWNDVWTGPTAEARRWLLDQVERMTGRSAEVEEHSAGVRPT
                     SRDRRPLLGRIAQHAAVFNGLGSRGVMLAPWCAQHLADHVLDGVPLDPEVDVARFAS"
BASE COUNT          489 a          863 c          963 g          515 t
ORIGIN      
        1 gcatcggtgg tgacgtcgtg cttcgcgacc tcatccccgt tcacatcgac gaagcggacg
       61 gacgtatgat acgaggcctt ggtgatggtt gtgcctgcgc gccgcacgga aacgatccct
      121 ttgtaatgga cctcctgtcc cggccggaag atggcccggt cggtgaagag gaaggtgcgg
      181 agcgtgtcgc ggtccggatc ctccacctgg taccagccat agctgctgat cctctgttgg
      241 tcgccggcat ggctcacctt ccaggtgtac tgcccgcgga gatcattcgg aacggggacc
      301 cgggctatgc cttgctcatc cgtaagggcg gagccgatgg gcaggtcgcg gtaagtgcca
      361 ttcgaccagc gctgcgagaa gagttcggct ttcgcggagc gcaatggtgc acccgacacg
      421 cggtcggtga cgagcacgct cgtcatccgg tcaggctgct cacgggtggt cagcgccaga
      481 cgggtgatgg tgaacggggt gaacaggacg agtgcaccat ccagtgcgaa gctctcggca
      541 tcgctcacga tcaactggta gttgcccagc gggagcccat ccacgggcag ctcggaacgg
      601 tgcgcattca ggtcgccgtc gtccgtgagc aaagcactcc atgcacgcac ggccggcgac
      661 ttcagcagcc gtttgaagcc atcctcatcg cgcgaacggt gctcgtcgcg gtcgtccttc
      721 acgatgcgga gccagacctg ttccacattg gtgtgggtga gtgctgcgcg gaacggcaca
      781 tcgggtgtgt tcgcttcctc cacctccacg ctcaaggcgg gcatgaccag ccgtgcgagc
      841 agcgcctccg cattgctggc gccgaaggac cccggccatt tggcgatcga ggtgcggcag
      901 aggtcggcag cggtacgttt ttcggtcttc cacgcacccg atggatcgag gcgctggtat
      961 ttttcaccct ggtccgcgtg ccattgcgcg atggccgtga tcacttcggc ctcgcaggta
     1021 tcgttgggca ggcgcgagcg caggagttcc agtgccccga ggtacaggga gtccttctgg
     1081 ggcaggccgc tccgttcccg cacgaagtgc aagcggtcga gcgtgatgtt caccaacgca
     1141 tcgggccttg catcgatcag gtgctggcgt tccagccgtt ggaagagttg caatgcctgg
     1201 aactccctgg cggtactgtc gcgatgggcg agtggcttgt gcgcgaaagc ctcgaacagc
     1261 gagaagaagg ccgggtcgtc cagctggaaa cgccaggtgg gctcggaaag tcgtgtttcg
     1321 gagttgcgga agatgtccag tgcccgacgg gccaggatgt cgtggaccgt tgggcagagg
     1381 atcgtttcgg cacctcgttc cggcagcaga agaggtgcga ggtcgccaac gggcatcgcg
     1441 gcgagcgtat cgcacggatc gagcgatgcg cggtaggcat cgatcacttt ccgcatgaag
     1501 gtgcgctggt cccaggtgtc gggatcatcg cccggctcat cctgcgccgt gcgttcgagg
     1561 atgcgccagc ggtcgtcctg gtaccaaagc cagtacgact cgccgatcac cgatcggaga
     1621 aggggtttga gcggcaggcc gacggtcgtg ttcgcacgct gttccatggc cgcgatcacc
     1681 tccttgcgtt ccacaccgat ggcgcgctgg aagcggccct tgtacatcca cgcacggaat
     1741 tcggtcagtt ggtcgccgtc gagctgtgcc tgtgacagga tcgttcccgt ggcgtcgagc
     1801 gcgctggcga actggccgat cgcggcgagt gaatcgatgg cggcccagcg cgggtcggga
     1861 acaggaacgt tggtgatcac ggcctttttc gttgtggagc aaccgagcac cgttgcaccg
     1921 atgaccaggg tgccgaacag gatctttttt gcgaggagca tcagggaaga acgccttccg
     1981 accggagggg ccgggcgttg cgggcaaagc tcgtgcattg ggcgtgacat cgctgtgcga
     2041 tgcggtacac gcgtgtccgc ttcgcgttca ggtgggccct atgaaggatc ggcgggggcg
     2101 gccaggcgat gtccgggctt gtatgcagcg caagtggacc ggtcggtacc ggatgtcggg
     2161 aaggaacaag tacggactac gaggcgaacc gcgccacatc cacttcggga tccaacggaa
     2221 caccatcgag cacgtggtct gccaggtgtt gggcgcacca gggggccagc atcacgccac
     2281 gcgagcccag gccattgaac acggcggcat gctgagcgat ccgccccagc aatggacggc
     2341 ggtcgcgcga ggtgggtcgc acgccggccg agtgttcctc cacttcggcc gagcgaccgg
     2401 tcatgcgttc cacctggtcc agcagccacc gccgggcctc ggcggtggga ccggtccaca
     2461 catcgttcca ttcgaaggtg gaacccacgc gataacgatc ctctcccaat gggataagga
     2521 acacgccccg atgcaccacg ctcttgagcc ggaggccggg tatgcgcacg gtgagcatgt
     2581 caccgcgcac ctgcgagagg ccatcgatcc gcgcgaacgg gccctcgcat cgcacgagca
     2641 tcggggccga atcctcaccg atgcgcacgc cgccttcctc atggatgatg tcgttgtcct
     2701 cgacggtccg ttcgaccagt gcgcccgatc ccagccagcg ttcgcggtgc gcgtccagca
     2761 tgcggggcag gtcgagccgt gcgcaacggt tcacgatgcc gtggccgtag ggtcgttcca
     2821 atccctctat
//
LOCUS       DAOQOC010000296         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19311_length_2828_cov_3.099171, whole genome shotgun sequence.
ACCESSION   DAOQOC010000296 DAOQOC010000000
VERSION     DAOQOC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19311_length_2828_cov_3.099171"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PL106_05830"
     CDS             <1..534
                     /locus_tag="PL106_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA32623.1"
                     /translation="TASVAAHNVLLDDGCLRGFDTHYKTVPPLRDMEHVNALREGVKD
                     GTIDTITSDHRPEDIEHKKVEFPRAAPGIIGLETAFAVANTALKGKMPTRKLVERLCH
                     GPRAALGLPVPHIAEGTEAEMSLFLPDLDWTFGDADIVSRSRNTPFIGYHFTGRAAGI
                     VSNGQLVLVPGLLEAVN"
     gene            complement(614..1183)
                     /locus_tag="PL106_05835"
     CDS             complement(614..1183)
                     /locus_tag="PL106_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32624.1"
                     /translation="MKLVQNTCAAACLALVACGGATKEDKSPAIDALITEARSRVGAM
                     EDSLSDAASFDRRRLMALRDVYVAYADRFPQDSLAPDMLRKAAATSGDLNEHEKAVGL
                     YDRLIKNYPQWPRTADAMYFRAFEIENGLGKKGEAKAAYEDFMKKYPENQFADDAAKM
                     IEYMSLTDAELIERFQKMNEGTEEGSAGQ"
     gene            complement(1192..1779)
                     /gene="def"
                     /locus_tag="PL106_05840"
     CDS             complement(1192..1779)
                     /gene="def"
                     /locus_tag="PL106_05840"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNA32625.1"
                     /translation="MVLPIRAFGDPVLKKVASDIEQGHAGLDQLVKDMFETMYAANGV
                     GLAAPQVGQSIRLFVVDTHAGSDDEDGEAAPEGMEPEPRLKRVFINAYIVEEEGQEWG
                     FEEGCLSIPGIREEVDRQPRITLRYQDEKFEEHEEEFVGFAARVIQHEHDHLDGILFT
                     DHLPPLRKRLIQGKLRDISMGKTDAKYPMRFVPKK"
     gene            complement(1807..2235)
                     /gene="ruvX"
                     /locus_tag="PL106_05845"
     CDS             complement(1807..2235)
                     /gene="ruvX"
                     /locus_tag="PL106_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNA32626.1"
                     /translation="MEPKRVLAIDLGLKRTGLAVTDPARIIASALDTVSTNELLAYLT
                     RYLARTPLEGFVIGLPVGLDGRDTDATPHVRKFIVQLRKTFPDQWVETVDERYTSSLA
                     RQTVLASGKGRTARQDKGALDRISATIILQGWLDRHALRR"
     gene            2328..2828
                     /locus_tag="PL106_05850"
                     /pseudo
     CDS             2328..2828
                     /locus_tag="PL106_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685738.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
BASE COUNT          503 a          898 c          878 g          549 t
ORIGIN      
        1 acggccagcg ttgccgcgca caacgtgctg ctggacgatg gctgcctgcg cgggttcgac
       61 acgcactaca agaccgtgcc cccgctgcgc gacatggagc atgtgaacgc cctgcgcgag
      121 ggcgtgaagg acggcaccat cgataccatc acaagcgacc atcggcccga ggacatcgag
      181 cacaagaagg tggagttccc acgagccgct cccgggatca tcggcctgga aacggccttc
      241 gccgtggcca ataccgcgct gaagggaaag atgcccaccc gcaagctggt ggaacgcctg
      301 tgccatggcc cgcgcgcggc cctcggcctc cccgtaccgc acatcgccga aggcaccgag
      361 gccgagatgt cgctgttcct gcccgacctc gactggacat tcggtgacgc cgacatcgtg
      421 agccgctccc ggaacacccc gttcatcggg tatcatttca ccggccgggc cgcgggcatc
      481 gtcagcaacg ggcaattggt gctggtaccg ggtctgttgg aagccgtgaa ctgaggcccc
      541 ggttaccatg cccggatccg aaccgggacg gatgccggat caaccacggg gatccattga
      601 ccgtgtacac gacctactgc cctgcactcc cttcctccgt gccctcattc attttttgga
      661 accgctcgat cagctcggca tcggtaaggc tcatgtactc gatcatcttg gcggcatcgt
      721 cggcgaactg attctccggg tattttttca tgaagtcctc gtacgcggcc ttcgcttcgc
      781 ccttcttgcc gagtccattc tcgatctcga acgcccggaa gtacatcgcg tcggccgtac
      841 gcggccattg tggatagttc ttgatgagcc ggtcgtacag gcccacggcc ttctcgtgct
      901 cgttcaggtc gcctgaagtg gcggccgcct tgcgcaacat gtcgggggcc aggctgtcct
      961 gcgggaaacg gtcggcgtac gccacgtaga cgtcccgcaa ggccatcagc cgtcggcggt
     1021 cgaagctcgc ggcatcggag aggctgtcct ccatggcacc cacccgggac cgggcttcgg
     1081 tgatcaaggc atcgatcgca gggctcttgt cctccttcgt ggccccaccg caggccacca
     1141 gcgccagaca ggccgcagcg catgtatttt gtacgagttt catcggatgt ttcatttctt
     1201 cgggacgaag cgcatggggt atttcgcatc ggtcttcccc atgctgatgt cccgcagttt
     1261 cccctggatc agccgcttgc gcaagggtgg caggtggtcg gtgaacagga tcccatccag
     1321 gtggtcgtgc tcgtgctgga tcacacgggc ggcgaatccc acgaactcct cctcgtgctc
     1381 ttcgaacttc tcgtcctggt agcgcagggt gatccgtggc tggcgatcca cctcctcccg
     1441 gatgcccggg atgctcaggc agccttcttc gaagccccat tcctgtcctt cctcctccac
     1501 gatataggcg ttgatgaaga cccttttcag gcgcggctcc ggctccatgc cctcgggcgc
     1561 ggcctcgcca tcctcgtcat cggatcccgc atgtgtgtcc accacgaaca accggatact
     1621 ctgccctacc tgcggcgcgg ccagccctac gccattcgcc gcgtacatcg tttcgaacat
     1681 gtccttgacc agctgatcga ggccggcatg gccctgctcg atgtcagagg cgactttttt
     1741 cagtacgggg tcgccgaagg cgcggatggg aaggaccatt gggtgcggcg aatttaacgg
     1801 ggcgactcac ctgcgaagcg catgtcgatc gagccagcct tggagaatga tcgtggcact
     1861 gatacggtcc agcgcgcctt tgtcctgccg ggcggtgcgg cccttgccac tggccagcac
     1921 ggtctgccgg gccaggctgc tggtgtatct ctcgtccacc gtttccaccc actgatccgg
     1981 aaaggttttg cgcagctgga cgatgaactt gcggacatgg ggcgtggcgt ccgtatcacg
     2041 tccatccagg ccaacgggca acccgataac gaagccttcc agcggcgttc gcgccagata
     2101 gcgcgtcaga tacgccagga gttcattagt ggacaccgta tccaacgcgc tggcaatgat
     2161 gcgcgcagga tcggtcacgg cgagtccggt acgcttgagg ccgaggtcga tcgcgagcac
     2221 gcgcttgggt tccactggtg cgttggttgg tcgggcaccg caaagaacgg gcggatcccc
     2281 atggcatatt tgcccaccgt tacggcctat cgctcggacg gcaccagatg cacgaaatga
     2341 tcgacctggc ctggaaggac cgcaacctgc tgcgcgatgc ggacgtcgtg agccacatcg
     2401 aggccattat tgagaagctg gaccgtgggg agatccgcgt ggcggagcca ccgaaagggc
     2461 cgaatgtgga gtggaccgtg aacgactggg tgaagaaggc ggtgatcatg tacttcccca
     2521 tccggcagat gcacacgatc gaactcgggc cattggagtt ccacgacaag atgaaactga
     2581 agcgcggcta cgcccaaatg tgagtgcgcg tggtgccgca tgccgtggca cgctatggcg
     2641 cgtacctcgc ccgcggagtg atcatgatgc cgagctacgt gaatatcggt gcgtacgtgg
     2701 acagcgatac catggtggac acttgggcta ccgtgggcag ttgtgcacag atcggcaagg
     2761 gcgttcacct cagtggcggc gtgggcatcg gcggcgtgct ggaacccgtg caggccgctc
     2821 cggtgatc
//
LOCUS       DAOQOC010000297         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19323_length_2827_cov_3.073232, whole genome shotgun sequence.
ACCESSION   DAOQOC010000297 DAOQOC010000000
VERSION     DAOQOC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19323_length_2827_cov_3.073232"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2772)
                     /locus_tag="PL106_05855"
     CDS             complement(<1..2772)
                     /locus_tag="PL106_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HNA32627.1"
                     /translation="MRSRRVLLASSAFALGLVVRGQYLPEFNMTDTVVTACKGILLDS
                     ELGPNEELYSNNEDFTFTINTGSTITLIFDDVFCLEQNYDFLTFHDGPTIASPQIGPV
                     YSGIIAPPPVVATSGQLTVHFVSDATVAYCGFEAQWTSVVAPPVPPVMTIPVAPSCGS
                     PTLGIALSYPLPCDSLYRDAFAISGMGDPAITAIAPDCSGADASQIQLTTDPAFERNC
                     PYTVTFTCMLPDACDSLWTFVLTATTQVTTCPIAVDIVTAFDTVCAGSCTQLTADVNG
                     CLTYGYSWSNGLPGTESVNVCPTATTTYSVSVTENGTGATATDDITIVVLDPQITATP
                     MTVCQSLEAFDLTATPPGGTWSGPGIIDSLAGTFDPDTSGPGTHIIHYTGIGGCFDEI
                     IIAVDSMDAGLPQAACPGSTPFLQTGATPQGGIWSGPFIQPDGLFDPNTAGSYIVTYT
                     AGNCSDTIRINVDDIAGQTQLDTVCQSTWPFIIPASPFGGRWSGPGITDTIMGVFDPD
                     EAEGGTHTLQYAMHGCDLSFTIHVKPVDIGDDRSACPSQGLITPDPAAVPPGGVWSGD
                     GIVDASIGTYDPVQAGNGWDQLTYAAPNGCTDTIGILVGWTEVDDDTLFFCSGDEALI
                     LDENTTGRTPWDGAWSGSGLFQNDDDEWFFDPAVVGTGMFILHFDANSCGDSLIAIVH
                     PSSLGTQDMELCGATGPFVIAPVPPGGTWSGDGIIDSESGVFSPAEAGSGAHIIRFDA
                     PAGCGDSLTITIQPFQQASIGGVEEMYCGNDMPVEIQLFPPGGQFTGLNDTTFNPAYL
                     DPGTYTLVYSTGFGACSSSDTVIFTNHAPLTTLFLVSQNPICGGGGSVLTVVPSGGLP
                     GASISFQWDHGLFPIAQQSVIPDSSDTYIVMTTDGCSDPVIDSVTLVVHPPFFPAFSF
                     SDTLCHGEPG"
BASE COUNT          536 a          794 c          972 g          525 t
ORIGIN      
        1 cccgggttcg ccatggcaca aggtatcgct gaagctgaac gctgggaaga acgggggatg
       61 gaccacgagg gtcacgctgt cgatcacggg gtcggagcag ccatcggtgg tcatcacgat
      121 gtatgtatcc gaagaatcag gtatcaccga ctgctgtgcg atggggaaga ggccatggtc
      181 ccactggaag gagatgctgg ccccgggaag gccaccgctg ggcaccacgg tgagcaccga
      241 gccgccaccc ccgcatatcg gattttgcga gaccaggaac agcgttgtga gcggagcatg
      301 gttggtgaag atcacggtat cactgctgga acaggcgccg aaacccgtgg agtagaccag
      361 ggtgtacgta cccgggtcca ggtatgccgg gttgaacgtg gtgtcgttca gcccggtgaa
      421 ttgtcccccg ggagggaaga gctggatctc aacgggcata tcgttgccac agtacatttc
      481 ctccaccccg ccgatcgatg cctgctggaa tggttgaatg gtgatggtga gcgagtcgcc
      541 gcacccggcc ggggcatcga accggattat atgggcgcca ctgccggcct cggccgggct
      601 gaacacgccg ctttcactgt cgatgatgcc atcgccgctc cacgtgccac cgggcggcac
      661 gggagcgatc acgaacggac cggtcgcacc gcaaagttcc atgtcctgcg ttccgaggga
      721 tgaaggatgc acgatcgcga tgagcgagtc gccgcaggaa ttcgcgtcga agtggaggat
      781 gaacatcccg gtgcccacta cggccggatc gaagaaccat tcgtcatcgt cattctggaa
      841 gagaccactg cccgaccagg cgccgtccca gggcgtgcgt ccggtggtgt tctcatccag
      901 gatcagggct tcatccccgg agcagaagaa gagcgtgtca tcatccactt ccgtccatcc
      961 caccaggatg ccgatggtgt ccgtacatcc gttcggagcg gcgtaggtga gctggtccca
     1021 gccgttgccg gcctgaacgg gatcgtacgt gccgatggat gcgtccacga tcccgtcccc
     1081 gctccacaca ccgcccggtg gaacggcggc gggatctgga gtgatgagcc cctgtgatgg
     1141 gcatgcgctg cggtcgtcgc cgatgtccac gggtttcacg tggatggtga acgacaggtc
     1201 gcacccgtgc atggcgtact gcagcgtgtg cgtgccgcct tcggcctcgt ccgggtcgaa
     1261 tacgcccatg atggtatcgg tgatgccggg cccgctccat cgaccgccga agggcgatgc
     1321 gggaatgatg aagggccagg tactctggca cacggtgtcc agttgcgtct gcccggcgat
     1381 atcatccacg ttgatgcgta tcgtgtcgga acagttcccg gcggtgtacg tgacgatgta
     1441 cgagccggcc gtgttcgggt cgaataggcc atcgggttgg atgaagggcc cgctccatat
     1501 accgccctgc ggggtggcgc ccgtctgcaa gaagggggtg gagccggggc atgcggcttg
     1561 gggaagacca gcgtccatgc tgtccacggc gatgatgatc tcgtcgaaac aaccaccgat
     1621 cccggtgtag tggatgatat gggttcccgg gccagaggta tcggggtcga aagttcccgc
     1681 aagcgagtcg atgatccccg gaccgctcca cgtgccgccc ggtggcgtgg cggtcaggtc
     1741 gaacgcctca agtgattggc acaccgtcat gggtgttgcg gtgatctgcg ggtccagcac
     1801 aacgatggtg atgtcgtccg tggcggtggc tccggtcccg ttctccgtga ccgaaacgct
     1861 gtaggtggtg gtggcggtgg gacagacatt cacactttcc gtaccgggca ggccgttcga
     1921 ccaggagtag ccgtacgtga ggcaaccatt cacatcggcg gtgagttggg tgcatgatcc
     1981 ggcacaaaca gtatcaaatg cggtaacgat gtccaccgcg atagggcagg tcgtcacttg
     2041 tgtggtggcg gtcagaacga aagtccacaa gctgtcgcac gcatcgggca acatgcaggt
     2101 gaaggtcacc gtgtacgggc agttgcgctc gaacgcgggg tccgtggtga gttggatctg
     2161 cgaggcatcg gctcctgaac aatcgggtgc gatggcggtg atcgcgggat cgcccatccc
     2221 gctgatcgcg aacgcatcgc ggtacaggga gtcgcagggc aggggatagg agagggcgat
     2281 acccagggtc gggctgccac aggagggcgc gacgggaatg gtcatcaccg gtggtacggg
     2341 cggcgccacc acgctggtcc attgggcctc gaatccgcag tatgccacgg tggcatcgct
     2401 cacgaaatgc accgtcagct gtccgctggt cgcaacgacc ggagggggag cgatgatgcc
     2461 agaatagacc gggccgatct gtggcgaggc gatggtgggt ccatcgtgga aagtcaggaa
     2521 gtcgtagttc tgttccaggc agaacacatc atcgaagatc agcgtgatcg tgctgcccgt
     2581 gttgatcgtg aaggtgaagt cctcgttgtt ggagtagagt tcctcgttgg gtcccagttc
     2641 gctgtccagc agtatgccct tgcaggcggt caccaccgtg tcggtcatgt tgaactccgg
     2701 caggtactgt ccacgcacga ccaacccgag cgcgaacgca ctggaggcga ggagcacgcg
     2761 tcgggagcgc atcgatcaga ggctgtcgcg gttgatggtg atggtccccg tggtgaactc
     2821 attgctg
//
LOCUS       DAOQOC010000298         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19441_length_2819_cov_3.064761, whole genome shotgun sequence.
ACCESSION   DAOQOC010000298 DAOQOC010000000
VERSION     DAOQOC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19441_length_2819_cov_3.064761"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PL106_05860"
     CDS             <1..734
                     /locus_tag="PL106_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA32628.1"
                     /translation="LLCNPGNPTGYLYTRAELEGLSALVKEHGLYLFSDEVYREFCYD
                     GAHHESAMTLPGLEQHVVLIDSVSKRYSMCGARVGAFITRNAELLATVLKFAQARLSP
                     PTFGQVASEAALHTAKSYFDEVVKEYTLRRDILVDGINSIPGAVCPRPKGAFYCVAEL
                     PIDDSDTFCQWLLESFAHKGATVMMAPATGFYSEPAPGRKQVRLAYVLEADKLRLAVE
                     CLAVALEQYPGRTRDAHPAGKMVRN"
     gene            complement(741..1475)
                     /locus_tag="PL106_05865"
     CDS             complement(741..1475)
                     /locus_tag="PL106_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNA32629.1"
                     /translation="MNTNVHLKRLRALHRRKGREEQGLFLVQGRKPVSELLASPFNID
                     SIHATERAARELGLRQALIWPEHDLERMGTLESGNEVVAVVRTPPNERPRVPKGLTIA
                     LDGISDPGNLGTLVRIADWFGVEQVWCSTDCTEVWNPKCVQAGMGSVFRVPVRYGDLA
                     AAILEAVGADVSVYKAEASGANVFGMELRRPAVLVLGSESHGLRDAVRNGPGRSVAVP
                     RYGSAESLNVAAAAAALCMEFIRTNR"
     gene            1541..>2819
                     /locus_tag="PL106_05870"
     CDS             1541..>2819
                     /locus_tag="PL106_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32630.1"
                     /translation="MPCARNERLHIPLLLGVLMLAACNTTRWVPPGEHLLTRNVITIS
                     PKGTLTTEELEPILKQKPNKRVLGRAIYLDLYNLRDPANVARKRLLKDSLCEAENAAR
                     RLIDSSRTKECNASQRERNGEAPVLLDPVLVERTVEQLRNYAFKEGYFDAQVNDTVRH
                     VRRRWPFGGWGRPYHKPKASVEYRVDAGQPWTLCTINLRVDDPAIASHLRSDTTGSLL
                     HPGDRFDGDVLEAERVRITNELKKEGYLFFNKDMVIFDADTSAGDHEVDLLIRLERPL
                     ASTQRGLKGTPPGTRWYVNDVYIDIAHRVKEPEHGYDTLRYDGFTLLHKGRHHDFRPG
                     PITSAVLLKPFSRFNQVDADRTFRRLANMGVFDRVDISFDTLGLGPKNVDARITLIPS
                     RRQGFSVEGFLTNRGGFLGASGSLNYRHKNLFRT"
BASE COUNT          543 a          968 c          830 g          478 t
ORIGIN      
        1 tcctcttgtg caaccccggc aaccccaccg gctacctgta cacccgtgcc gaactggagg
       61 gactgagcgc actggtgaag gagcacggcc tctacctctt cagcgatgag gtgtaccgcg
      121 agttctgcta cgatggggcc catcacgaga gcgccatgac cttgcccgga ctggagcagc
      181 acgtggtact catcgacagc gtgagcaagc gctacagcat gtgcggggca cgtgtcggcg
      241 ccttcatcac gcgcaacgcc gaactgctgg ccaccgtgct caagttcgcg caggcacgcc
      301 tgagtccgcc caccttcgga caggtggcct cggaagccgc cctgcacacc gcaaaaagct
      361 actttgacga ggtggtcaag gagtacaccc ttcggcgcga catcctggtg gacggtatca
      421 attccattcc cggcgcggtg tgtccccggc ccaagggagc gttctattgc gtggccgaac
      481 tgcccatcga cgacagcgat accttctgcc agtggttgct ggaatcgttc gcgcacaagg
      541 gagccacggt gatgatggcc ccggccaccg gcttctacag cgaacccgcc ccgggacgga
      601 aacaggtcag gctggcgtac gtattggagg cggataaact tcgactggcc gtggagtgtc
      661 ttgccgtggc gctggaacaa taccccggtc gcacccggga tgcgcacccc gccgggaaga
      721 tggtccggaa ctgaggatcg ctaccggttc gtccggataa attccataca gagcgccgcc
      781 gcggccgccg ccacgttgag cgactccgcc gatccgtagc gtggcaccgc gacgctccgg
      841 cccggcccgt tccgcaccgc atcacgcagt ccgtgcgact cgcttcccag caccagaaca
      901 gcgggccgcc ggagctccat gccgaacacg ttcgcaccgg aagcctcggc cttgtacacg
      961 ctcacgtccg cgcccacggc ctccaggatc gccgccgcca ggtcgccata gcgcacggga
     1021 acacggaaca ccgagcccat gccggcctgt acgcacttgg ggttccacac ttcggtgcaa
     1081 tcggtcgagc accagacctg ttcaaccccg aaccaatccg ctatgcggac caaggtgccc
     1141 aggttgcccg gatcgctgat accatccaag gcgatggtga ggcccttcgg cacacgtggg
     1201 cgctcgttgg gcggcgttcg caccacggct accacttcgt tccccgactc gagcgtcccc
     1261 atccgctcca ggtcgtgctc cggccagatc aacgcctgac gaagcccaag ctcacgcgcc
     1321 gcgcgttccg tggcatgaat actgtcgatg ttgaacggtg aggccagcag ttccgatacc
     1381 ggcttgcggc cctggaccag gaaaagtcct tgttcctcgc ggcctttccg gcgatgcaag
     1441 gcccgcagcc gcttcaggtg cacgttggtg ttcatggctg cggatatctt cgggcgcgtt
     1501 cctgccaatg gatggaaagc cgcgaaaaat agcccctccc ttgccctgcg cgcggaacga
     1561 acgcctccat atcccgctgc tcctgggcgt actcatgctc gcagcgtgca acaccacgcg
     1621 ttgggtccct cccggcgaac acctgctcac gcgcaacgtg atcaccatct cgcccaaggg
     1681 cacgttgacg acggaggaac tggagcccat cctcaagcag aagcccaaca aacgtgtgct
     1741 cggaagggcc atctacctgg acctgtacaa cctgcgtgat cccgcgaacg tggcgcgcaa
     1801 acggctcctg aaggacagct tgtgcgaagc cgagaacgcg gcccgccgcc tcatcgacag
     1861 ctcgagaacg aaggagtgca acgcatcgca acgcgaacgg aacggtgagg ccccggtgct
     1921 tctggacccc gtgctggtcg aacgcacggt ggagcagttg aggaactacg cgttcaagga
     1981 agggtacttc gatgcgcagg tgaacgacac cgttcggcat gtccgccgtc gttggccttt
     2041 cggtggttgg gggcggccat atcacaagcc caaagcgagc gttgaataca gggtggatgc
     2101 cgggcaaccc tggaccttgt gtaccatcaa cctgcgggtg gacgaccccg cgatcgccag
     2161 ccatctccgt tccgacacca ccggctccct gctccacccg ggtgatcggt tcgatggcga
     2221 cgtgctcgaa gcggaacgtg tccgcatcac caatgaactg aagaaggaag gctacctgtt
     2281 cttcaacaag gacatggtga tcttcgatgc ggatacctcg gccggcgacc acgaagtgga
     2341 cctcctgatc cgcctggagc gcccgctggc ttccacccag cgcggactca agggcacacc
     2401 acccggcacg cgctggtacg tgaatgacgt gtacatcgac atcgcccatc gcgtgaaaga
     2461 gcccgagcac gggtacgaca ccttgcgcta cgacgggttc acgctcctgc acaaaggccg
     2521 tcatcacgac ttccggcccg gccccatcac ctcggccgtg ctcctcaaac ccttcagccg
     2581 cttcaatcag gtcgatgcgg accgcacctt ccgacggctg gccaacatgg gtgttttcga
     2641 ccgggtggac atctcattcg acaccttggg cctgggcccg aagaacgtgg atgcccggat
     2701 caccctgatc cccagcaggc gtcagggatt ctcggtcgaa ggtttcctca ccaaccgggg
     2761 cggcttcctc ggcgcctcgg gcagcctcaa ctaccgccat aagaacctct tccgcaccc
//
LOCUS       DAOQOC010000299         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19573_length_2810_cov_3.117241, whole genome shotgun sequence.
ACCESSION   DAOQOC010000299 DAOQOC010000000
VERSION     DAOQOC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19573_length_2810_cov_3.117241"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="PL106_05875"
     CDS             <1..1954
                     /locus_tag="PL106_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32631.1"
                     /translation="VLSPGAGYLIEYGPSGFTPGSGTTSTGTQGDTQPPVIISGLSEG
                     QVYDFYISSGCGGGDPSPVTGPWAAIAGGNTYPNDDCGNDQPITCGSITNGSTLLAVE
                     DGTPECVTSVSAPGVWYGISDLGGTVVLSTCDGSNYDTKIVVYTGPCDDLVCVTGNDD
                     GPFDCEFGSDVVLNAEAGTAYHVLVQGYNGAVGDFALAMNCSDCSLPTNVSVVAADDF
                     AVMNWITANADGTFSLEFGPDGFAPGTGTVISGTVGVDGPPVTIAGLTLSTDYDLYLT
                     EDCPGGAGIRRGPWSFTTLDTAPEDNAFCETAIGLTCGSTAEGNTAMGVLATLPYCGS
                     AYVTAPGLWYTFTGDGNDVTLSTCNDAEFDTRISVFSGECPAPVCVAGNDDAPGCGGN
                     TSSVTIHTENGASYLAYVHGYNGATGAFTISMNCAAPCSPAVSGDDCTTAITLVPQPL
                     SDCVPTTGTNVCAYEGPLPNPDCDPYSAAHDVWYAINTGPSSDHTITVATVTASPVLV
                     ALYGSCDEAGYIACHDPSEGAIVLTGLDQSATYYLRVWNEGGPDAGTFTICDEAPIVT
                     RIDEPGSDAPLRVWPVPVDDVLALDGVPTGSERLVVRDTQGRQVGSKRVDRTGTMTLD
                     LGMLAPGAYTVHVIGPQPQVVHVVVR"
     gene            complement(2206..>2810)
                     /locus_tag="PL106_05880"
     CDS             complement(2206..>2810)
                     /locus_tag="PL106_05880"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32632.1"
                     /translation="ENRSSSKSVMRIDLPVPDGKRKLSIINTGKNLIYARVVRTGTPM
                     PGDELAANNGLALDVSYQTMNGQRIDPASIEQGTDFQAVVTVRHPGIRGQYQELALTQ
                     VFPSGWEIRNSRLEGTERAQTNSSYEYQDIRDDRVMTYFDLDARQQVTYRVFLNAAYT
                     GRFYLPAVTCAAMYDNTVNARSKGQWINVLKAGDSGTAKK"
BASE COUNT          480 a          840 c          912 g          578 t
ORIGIN      
        1 ggtcctgtcg ccgggcgcag gatacctcat cgaatacgga ccctcgggct tcacgcccgg
       61 atcaggcacc acatccacgg gcacgcaggg cgatacgcaa ccaccggtga tcatttcggg
      121 cttgtcggag gggcaggtct atgacttcta tatctcctcg ggatgcggtg gcggcgatcc
      181 gtcgcccgtc acaggcccat gggccgcgat cgcgggaggg aacacctatc ccaacgacga
      241 ttgtggcaac gatcagccca tcacgtgcgg ctcgatcacc aatggcagca ccctcctcgc
      301 cgtggaggat ggcaccccgg aatgcgtcac ctccgtttcg gcgcccggtg tgtggtacgg
      361 catctctgac ctgggcggta ccgtggtgct cagcacatgc gacggcagta attatgatac
      421 taaaatagtg gtgtacaccg gcccgtgcga tgatctggtc tgcgtgacgg ggaacgacga
      481 tggccctttc gattgcgaat tcgggagcga tgtggtgctg aacgctgagg cgggcaccgc
      541 gtatcacgtg ctggtacagg ggtacaacgg tgccgtgggc gactttgcgc tggccatgaa
      601 ctgttcggat tgttcgttgc cgacgaatgt ctcggtggtc gctgcggacg acttcgcggt
      661 gatgaactgg atcacggcca atgccgatgg gaccttcagc ctggagttcg ggccggatgg
      721 ctttgccccg ggcacgggaa cggtgatcag tggaacggtg ggcgtggatg gaccgccggt
      781 cacgatcgcg ggattgacgt tgtccactga ctatgacctg tacctgaccg aggattgccc
      841 cggtggtgcg gggatcaggc gcggtccatg gagcttcacc acgctggaca cggccccgga
      901 ggacaatgcc ttctgcgaga ccgcgattgg actcacctgc ggatccacgg cggaagggaa
      961 cacggcgatg ggcgtattgg ccacgctgcc ctattgcgga tccgcgtatg tgaccgcccc
     1021 gggtctgtgg tacactttca ccggtgatgg gaatgatgtg acgttgagca cgtgcaatga
     1081 cgcggaattc gacaccagga tcagtgtttt ttcgggcgag tgcccggcac cggtgtgcgt
     1141 ggccggcaat gatgatgcgc cgggttgtgg tggcaacacg agttcggtga ccatccacac
     1201 ggagaacggg gcgtcctatc tggcctacgt gcatggttac aatggcgcca cgggtgcgtt
     1261 caccatcagt atgaactgtg ccgcaccctg ctcgccagca gtgagcgggg atgattgcac
     1321 caccgccatt acgctcgtgc cgcaaccgct ttccgattgc gttccgacca cgggcaccaa
     1381 cgtgtgcgcg tacgaaggtc ccttgccgaa tccggactgt gatccgtact ccgctgcgca
     1441 cgacgtatgg tacgcgatca acaccggccc gtcgtcggac catacgatca ccgtggctac
     1501 cgtcaccgcc tccccggttc tggtggcgct gtacgggtcg tgtgacgagg ctggttatat
     1561 cgcctgccat gatccctccg agggggccat tgtgctgacc ggcctcgacc aatccgcgac
     1621 ctactacttg cgcgtgtgga atgagggcgg acccgatgcg ggcaccttca ccatctgcga
     1681 tgaggcgccc atcgtcaccc ggatcgatga accggggagc gacgccccgt tgcgcgtttg
     1741 gcccgtgccc gtggatgatg tgctcgcctt ggatggcgtg ccaactggca gcgaacgcct
     1801 ggtcgtacgc gatacgcaag gccgccaggt gggatcaaag cgggtggatc gcacgggcac
     1861 catgaccttg gatctcggga tgctcgcgcc gggggcatac acggtccacg tgataggccc
     1921 tcaaccgcag gtggtacacg tggtggtgcg gtagtgccga tccggcatcc gtcctccctc
     1981 tttgcttgtc gttccagcat tacggtatca tatatcttcg gccgggtttc tccgccgcgc
     2041 aggtccacct acgggaccat ccggttgttg cgccctgcga aaggatcacg acgcatccct
     2101 ggaccggtct gcgagccgga cacctgtgga gcggtacatg gtgggtcgtc aaggatcccg
     2161 gacgtcacca agggaagctt tccctgggat caggagcctt gctgatcatt ttttcgcggt
     2221 accactgtcg cccgccttca gcacgttgat ccactgtccc ttgctgcgcg cgttcaccgt
     2281 gttgtcgtac atggccgcgc aggtgacggc cggcaggtag aagcgaccgg tgtacgctgc
     2341 attgaggaat acacggtagg tgacctgctg tcgcgcatcg aggtcaaaat aggtcatcac
     2401 ccggtcgtca cggatgtcct ggtattcata cgaactattg gtctgcgccc gttcggtgcc
     2461 ctccaggcgg ctgttgcgga tctcccagcc actggggaac acctgcgtga gcgccagttc
     2521 ctgatactgc ccacggatgc cggggtggcg tacggtcacc accgcctgga agtcggttcc
     2581 ctgctcgatg gaggctggat cgatgcgctg tccgttcatg gtctgatagg aaacatccag
     2641 cgccagacca ttgttggccg cgagttcatc gccgggcatg ggcgtgccgg tgcgcacaac
     2701 gcgtgcgtag atcaggttct tgccggtgtt gatgatggac agcttccgtt tcccgtcggg
     2761 cacgggcaga tcgatgcgca tcacgctttt gctgctggac cggttctccg
//
LOCUS       DAOQOC010000300         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19576_length_2810_cov_2.804719, whole genome shotgun sequence.
ACCESSION   DAOQOC010000300 DAOQOC010000000
VERSION     DAOQOC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19576_length_2810_cov_2.804719"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PL106_05885"
     CDS             complement(<1..1262)
                     /locus_tag="PL106_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32633.1"
                     /translation="MRCLFILGPLFLASALQAQPGVLDPSFGTGGVVRLHPDLYGNNG
                     WAVATLPDNSMLVAGHAGISNPTGFVAHLHQDGNLDTSFGAGGIRIIDAEDQTWVRAI
                     TALADGSLLVVGAVFPGDTLHALMLAKLTPTGDMDGTFGNGGIVQTTLNERPFTASNL
                     AVQPDGRIVVSGYMKDSTQYYNSVLLRYNEDGSLDPSFNDDGIIVLTNWPSDDEFQDV
                     TLMPDGSVIAVGSTALTGAYRSLMVKVDSTGAFDSDFGTNGQFLPIDGVENSWARGVA
                     VVGDAILVGGFLDVEPGQRDGYVAKFHGNGTIDAAFNGSGISTLNYNIFDTYEDIAVQ
                     PNGQLVLCGTTGTFNDMSHHVLLSRFNADGSPDADFNAQWLLSSGTLTDAQIAWDEAL
                     QPDGRIVLCGYESGNPSSMLVARCLPDA"
     gene            1338..2468
                     /locus_tag="PL106_05890"
     CDS             1338..2468
                     /locus_tag="PL106_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNA32634.1"
                     /translation="MPRIAHFSLFRLRVPLKKPFVTSLGPSYEADNVVVIVRTTDGLL
                     GFGECNPFWTINGETAETGLAVGTHLGKALIGHEATDIEGAHAQMDRLIFGNNSIKSA
                     FDIALHDISAQAADLPLWKFLGGKDRSDLFTDYTVSIGDPEQMAIDAEDIVNEGFPVI
                     KVKLGGARPGQRTGDGDLDIRRVRAIRSRIASIPLRIDANQGWDPDTAIRVLNALGDQ
                     GIQHCEEPIPRWQYMELRRVQEASPIPIMADESCCDHHDAQRLIELGSCQRFNIKLGK
                     SGGLFKARKIIALAEAAGMEVQIGGFLESRLAWSTAAALATTSPCAKYFDMDTPLMFR
                     DDPVEGGLAYGANGAITLPQGIGIGASIKERYRSGTQGIEVG"
BASE COUNT          562 a          896 c          836 g          516 t
ORIGIN      
        1 caggcatccg gtaggcaacg ggccaccagc atgctggaag ggttaccact ctcgtacccg
       61 cacagcacga tccttccgtc cggttgcaaa gcctcatccc aggctatttg tgcatcggtc
      121 agcgtaccgc tcgagagcag ccattgggcg ttgaagtccg catccggact cccgtccgca
      181 ttgaagcggg acaacagcac atggtgtgac atgtcattga acgtacccgt cgtgccgcac
      241 aacaccaatt ggccgttggg ttgtacggcg atgtcctcgt aagtgtcgaa aatgttgtag
      301 ttcaacgtgg agatgcccga tccgttgaat gccgcgtcga tcgtgccatt gccatggaac
      361 ttggccacat acccatcacg ctgtccgggt tccacatcca ggaatccgcc caccaggatc
      421 gcatcaccaa ccaccgccac gccgcgcgcc caggagttct caacgccatc gatcggcagg
      481 aactgaccat tggtgccgaa gtcgctgtcg aacgcaccgg tactgtccac tttcaccatc
      541 agcgatcgat aggctccggt cagagcagtg gagccgacag cgatcacact gccgtccggc
      601 atcagggtca catcctggaa ttcgtcgtcg gatggccaat tggtgagtac gatgatgcca
      661 tcgtcgttga agctcgggtc caagctcccg tcctcgttgt accggagcag cacgctgttg
      721 tagtactgcg tggagtcctt catgtacccg gacaccacga tccgcccgtc gggctgaaca
      781 gcaaggttgc tcgctgtgaa gggccgctcg ttgagggtgg tctgaacgat cccgccattc
      841 ccgaaggtgc cgtccatgtc gccggtcggg gtcagcttcg ccagcatcag cgcatggagc
      901 gtgtctcccg ggaatacggc tcccaccacc agcaggctcc catccgcaag ggcggtgatc
      961 gcgcgcaccc acgtctggtc ctcagcatcg atgatacgga tgcctcctgc cccgaaactg
     1021 gtatccaagt tgccatcctg atgcagatgc gctacaaaac cggtcgggtt gctgatacca
     1081 gcgtgcccgg ccaccagcat actgttgtca ggcaacgttg ccacggccca accattgttg
     1141 ccgtacaggt ccggatgcag ccggacaaca cctcccgtcc cgaaggaagg atccagcaca
     1201 ccaggttgtg cctggagggc agaggcgagg aagagcggac cgaggatgaa caggcagcgc
     1261 atgggaccgg tatttggaca tgtgaatgta gccgaagttc gtggacggcg atcgcttctc
     1321 tttggggacc gctacccatg ccacgcatcg cacacttctc cctcttccgc ctccgtgttc
     1381 cgctgaagaa acccttcgtc acttcccttg gccccagcta cgaggccgac aatgtggtgg
     1441 tgatcgtgcg aaccaccgac ggcctcctcg gcttcggcga gtgcaacccc ttctggacga
     1501 tcaacggtga aacggccgaa acgggcctcg cggtgggtac gcacctcggc aaggcgctca
     1561 tcggtcacga ggccaccgac atcgaaggag cgcacgcgca gatggaccgc ctcatcttcg
     1621 ggaacaacag catcaagagc gctttcgata tcgctttgca cgacatcagc gcacaagccg
     1681 ctgatctgcc gctctggaag ttcctcggcg gcaaggatcg ctcggatctc ttcaccgact
     1741 acaccgtgag catcggcgat ccggagcaga tggccattga tgcggaggac atcgtgaatg
     1801 aaggcttccc tgtcatcaag gtgaaactcg gcggggcccg ccccggtcaa cgaaccgggg
     1861 atggtgatct ggacatcagg agggtccggg ccatccgatc aaggatcgca tcgatcccct
     1921 tgcgcatcga tgccaatcag ggttgggacc ccgatacggc catccgcgta ctcaacgccc
     1981 tcggcgacca gggcatccag cattgcgagg agccgatccc ccgatggcag tacatggagt
     2041 tgcgccgggt gcaggaggct tcaccgatcc cgatcatggc cgatgagagt tgctgcgatc
     2101 accacgacgc tcaacgactg atcgaacttg gatcctgcca gcgcttcaac atcaagctgg
     2161 gcaagagcgg gggtttgttc aaggcacgga agatcatcgc cctggcggaa gcggccggga
     2221 tggaggtgca gatcggcggc ttcctcgaat cgcgcctggc ttggagcacc gccgctgctc
     2281 tggccaccac cagcccctgt gcgaagtact tcgacatgga tacgcccctg atgttccggg
     2341 acgatccggt ggaaggtggg ctcgcctacg gtgcgaacgg cgcgatcacg ctaccgcaag
     2401 ggatcgggat cggcgcttcg atcaaggagc ggtatcgatc cggaacgcag ggtattgagg
     2461 tggggtgatg ttcgtgttgc cctctggatc gatcccgcat tggcacctgg cggtcgcgga
     2521 ccggcaacag gaccggccag tagcatgcgg ttgctacgca gggaggcgcc aaaagcgccc
     2581 acctcgcatg gtccgcgcga caagcgcgga ccggcaaccg tgtcgaacgc ggacccggct
     2641 ggacttttcc cgcatcgtat tttcgcctcc catgagccag atcttcgacc tcgacatgat
     2701 caaagcggtg tacagccgct tccccgcacg catcgccgcc gcccgcaagg ccgtgggcaa
     2761 gccgctcacc ctcaccgaga agatcctcta cgcccatctg tgggacggcg
//
LOCUS       DAOQOC010000301         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19707_length_2799_cov_3.121356, whole genome shotgun sequence.
ACCESSION   DAOQOC010000301 DAOQOC010000000
VERSION     DAOQOC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19707_length_2799_cov_3.121356"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="PL106_05895"
     CDS             complement(<1..1817)
                     /locus_tag="PL106_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32635.1"
                     /translation="MGAERIILPISCALFMLGAHGQSLLQRHVDVHGRNIRLHEALES
                     VARDAGFQLAYNDAIVPGDSIVSIDATSTAEKALRSMLGEERVLKESGEHVIILEAPE
                     RKAIFGIEGFVRDHATGRPLAQASVREVNEKHVGITGADGSFRMRVSGRHERTALLVV
                     RTAYHDTVVFVGRDGQMGTVRLRPKERVEKMEPCHNAYCSTVDDLGITRLLVPANIQQ
                     QALNLAPEKRPIQISLVPTIGTNGKLSGAVSNAFSLNVVAGYAGGLHGVEVGGVVNMV
                     RRDVRGVQMAGMANLVGGHTNGVQLAGAINHTMRSFIGAQIAGLGNVVWDTLSGTQVT
                     GGVNVVKGGMRGTQISGLANLTTHSCDGAQVSSGVNIALGDVRKAQVSGLANYGRNVS
                     GTQFSLGANVALGEVGGGQVGSLANYAKVVSGGQVGFGINACIGEVKGGQVGLLNVAR
                     KCTGGQVGLINVSDTISGTSVGLLSFAWKGYHRVDLYATEMAPLTIAARTGTHRFYNL
                     FTYSPAVEDAGQWGFGYGSGTEFGHRGRHRGNLDWFAEHVNEQPQWIDAVNIVGHISI
                     TYGFVIQRRLIISGGPSFNVLFTDWRDAETGVYKSAIAP"
     gene            complement(1807..>2799)
                     /locus_tag="PL106_05900"
     CDS             complement(1807..>2799)
                     /locus_tag="PL106_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNA32636.1"
                     /translation="RNRTLSEQHHINSDQLARYVAGEASPEEARAVETWAGSSPLNAR
                     ELAAMRAAWDLSAPDPHARDVDVDAAWTKVRSRIGAGPGGAVIPIDRGRMVRWSRIAA
                     AAAAISGIILAAWWLIVPRPQEFATTGSSTTVFLADSSRVVLGPNSHLRASIGAERHI
                     ALHGQAYFEVTPDAQRPFVVEAHGLKVTVLGTAFTVEAYDSSSVVLARVRHGKVRVAA
                     SDGQQLDLLAGDHVRFEATTRRLERIAAPGMERWGDRILVFKGTPLPEVMDQIGSVYG
                     VKVYSSSKDPQGCKLTATFEDEPIDHVLRIVAETFGLTVTATAPGEYLLSGDGC"
BASE COUNT          507 a          956 c          822 g          514 t
ORIGIN      
        1 tagggagcga tggcgctttt gtacacaccg gtctccgcat cacgccagtc ggtgaaaagg
       61 acgttgaacg aaggcccgcc agagatgatg aggcgccgtt ggatcacgaa gccgtacgtg
      121 atactgatgt ggccgacgat attgaccgca tcgatccatt gcggctgctc gttcacgtgc
      181 tcggcgaacc agtcaaggtt gccccggtgc ctgccgcggt ggccgaattc ggtgcccgac
      241 ccgtagccga aaccccattg cccggcatcc tccaccgcgg gactgtacgt gaacaggttg
      301 taaaaacgat gggttccggt tcgggcggcg atggtgagcg gggccatctc cgtggcatac
      361 aggtccacac gatggtagcc tttccaagcg aagctgagca gccccacgct tgtgccggat
      421 atcgtgtcgc tcacattgat caaacctacc tgaccgcctg tgcattttct tgccacgttg
      481 agcaggccca cctgtccccc cttgacctcg ccgatgcacg cattgatccc gaagcccacc
      541 tgcccgccgc tgaccacctt ggcgtagttc gccagggatc cgacctgccc gccacccact
      601 tcgcccagcg ccacattggc gccgagggag aactgggtgc cgctcacgtt gcggccgtag
      661 ttcgccaggc cgctcacctg ggccttgcgc acatcgccca aagcgatatt caccccggaa
      721 gaaacctgcg cgccgtcgca cgagtgggtg gtgaggttgg ccagtccact gatctgggta
      781 ccacgcatgc cgcccttcac cacgttcaca cctccggtga cctgcgtgcc gctcaacgtg
      841 tcccagacca cattgcccaa gccagcgatc tgcgcaccga tgaacgaacg catggtatga
      901 ttgatcgcgc cggcgagttg cacaccgttg gtatgtccac cgaccaggtt ggccatgccc
      961 gccatctgca cgccacgcac atcgcgtcgt accatgttca cgacaccgcc cacttccaca
     1021 ccatgcaggc cccccgcata cccggccacc acgttcaacg agaacgcatt gctcacagcg
     1081 ccgctgagct tcccgttggt gccgatcgtg ggtaccagtg agatctggat gggcctcttc
     1141 tccggcgcga ggttgagtgc ttgttgctgg atgttcgccg ggaccaggag ccgtgtgata
     1201 cccaggtcgt ccaccgtact gcaataggcg ttgtggcagg gctccatctt ctctacgcgc
     1261 tccttgggtc gcagcctcac tgtgcccatc tgaccatcgc ggccgacgaa gaccacggta
     1321 tcatgatagg ccgtacggac gaccagcaac gcggtccttt cgtggcgtcc tgacacgcgc
     1381 atccgaaagg atccatcggc accggtaatg ccgacgtgct tctcattcac ctcgcggacc
     1441 gaagcctggg caagcggcct tcctgtcgca tggtcgcgca cgaacccttc gatcccgaag
     1501 atggccttcc gttccggggc ctcgaggatg atgacatgct cgccgctctc cttcaggaca
     1561 cgttcctcac ccagcatgct tcgcagggct ttttccgccg tgcttgtcgc atcgatggag
     1621 acgatgctgt cgccggggac gatggcatcg ttgtaagcga gttggaagcc cgcgtcacgg
     1681 gccaccgatt ccagggcctc gtgcaaccgg atattccggc catggacatc cacatggcgc
     1741 tgcaggagcg actgaccgtg ggccccgagc atgaacaggg cgcaggagat cggaaggatg
     1801 atccgctcag cacccatcac cgctcaacag atactccccc ggtgcggttg cggtaacggt
     1861 caggccgaag gtctcggcca cgatgcgcaa cacatggtcg atcggctcat cctcgaatgt
     1921 ggcggtgagc ttgcacccct gtggatcctt ggagctcgag tacaccttca caccgtacac
     1981 cgaaccgatc tgatccatga cttcgggtag cggcgtcccc ttgaacacca ggatgcggtc
     2041 gccccagcgc tccatgccgg gtgcggcgat ccgctccagc cgcctggtcg tcgcttcgaa
     2101 ccgaacatga tcaccagcga ggaggtccag ctgctgtcca tcggaggcgg ccacccggac
     2161 cttgccatgg cgcacccgcg cgagcaccac cgatgaactg tcgtacgcct caacggtgaa
     2221 cgcggtgccc agtacagtga ccttgagccc gtgagcttcc acgacgaaag gccgttgcgc
     2281 gtccggtgtc acctcgaaat aggcctgacc gtgcagggcg atgtgccgtt cagcaccgat
     2341 cgatgcgcgt agatgactgt tcgggcccag cacaacccgg ctgctgtccg caaggaacac
     2401 ggtggtgctg ctgcccgtgg tcgcgaactc ctgcggccgt ggtacgatca accaccaggc
     2461 ggccaaaatg atcccgctga tggcggcagc tgccgccgcg atccgcgacc agcgcaccat
     2521 acgaccacga tcgatgggga tcactgcgcc gcctggcccc gcaccgatcc gggaccggac
     2581 cttcgtccac gcggcgtcca cgtccacatc acgggcgtgc gggtcgggcg cggacaaatc
     2641 ccacgccgca cgcatggcag cgagctcgcg ggcgttcagc ggggaggatc ccgcccaagt
     2701 ctccacggcc cgggcttcct ccggagaggc ctccccggcc acgtaccggg ccaactggtc
     2761 gctattgata tggtgctgtt cgctcaatgt tctgttcct
//
LOCUS       DAOQOC010000302         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19756_length_2796_cov_2.582999, whole genome shotgun sequence.
ACCESSION   DAOQOC010000302 DAOQOC010000000
VERSION     DAOQOC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19756_length_2796_cov_2.582999"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..2109)
                     /locus_tag="PL106_05905"
     CDS             complement(331..2109)
                     /locus_tag="PL106_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNA32637.1"
                     /translation="MAIHRLFDIPDFQLAEYPQDVCVGSLEEGKLRTYNTREFIDTAE
                     QLALGLLDLGIAPGDKVALASGNRAEWAITDQAVLRIGAISVPIYPTMTADDYAYILE
                     HSGAKVFFVSNNDLFTKARAAQVRVPAVAHLFTYDKVPGASHWKELLGKGITQRAKLD
                     GCKAAVKREDLATIIYTSGTTGKPKGVMLTHDNILSNVEASAPRLPVKPGARAISFLP
                     LCHIYERMLMYLYQKAGTQVRFQETLDDLGARICEARPHVFTAVPRLLEKIYDTILAK
                     GEALTGIKRKLFFWSLDLGERYEVHGRSLWYDLQLGIASKLVFSKWREALGNEVACVA
                     SGSAALQPRLARIFNAAGIPLMEGYGLTETSPVVSVNDARNDGLRFGSVGRPLDKVEV
                     RIVPEPGHPEGEGEILIKGPNVMIGYYKEPELTREVLSADGWFRTGDIGKLEGGFLYI
                     TDRKKEMFKTSGGKYVAPQPIESALKASRFIEQAMVIGENRKFPSALIVPSFTFLKDY
                     CQLKGIPFTGNEQVISDERIHKRIFSEVGKVNEGLGHWEQVKRIVLLPGEFSVEGGEL
                     TPSLKLKRKAILAKYSGQVESIYAES"
     gene            complement(2214..>2796)
                     /locus_tag="PL106_05910"
     CDS             complement(2214..>2796)
                     /locus_tag="PL106_05910"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HNA32638.1"
                     /translation="RRIVRDFHRRFFNDERPRKLMLGINPGRLGAGSTGICFTDTKRC
                     ESDLGIPVHGMRTHEPSSDFFYRMVRSTGGADAFYRDVYVQAICPLGFTKAGSTGTQV
                     NLNYYDDKALQKAITPFVVQWLRRLIATGMRTDEVFCIGTGKNLAFLNTLNAEHGFFA
                     RITPLEHPRFIMQYRAQQLDTYVTKYVDALRTG"
BASE COUNT          464 a          880 c          874 g          578 t
ORIGIN      
        1 gttctcgttc atggcctgtt cgaccacgcg gccgcgcagt tcgctcttgc cgccctcgcc
       61 aggctctttc ttgcatccgg tgagcagcag ggcgagtgcg cccaacaggg aaagaagtgt
      121 tcgcatcatg ttccgttgaa ggtgcgcgaa tgtagaccaa ccgggagatg cgcatcctga
      181 tccctgcggg tcgttcgtgc ggtcatggtc atggccagaa ccacccgcat gaacacgatg
      241 gcccgcgcag ccgaggagcc tgccggcgct ggagatggtc gcctttcggg gccgtcatcg
      301 atccgatcga tcgcgtgggg caccgggcgt tcaggactcc gcgtagatcg attccacctg
      361 gccgctgtac ttggccagga tcgccttgcg tttgagtttg agcgatgggg tcaactcgcc
      421 gccctccacg ctgaactcac cgggcagcag gacgatcctc ttcacctgtt cccagtggcc
      481 caggccctcg ttcaccttgc ccacctcgct gaagatgcgc ttgtgtatcc gttcgtcgct
      541 gatcacttgc tcgttaccgg tgaaggggat ccctttcaac tggcagtaat ctttcaggaa
      601 ggtgaagctg gggacgatga gcgccgacgg gaatttgcga ttctcgccga tgaccatggc
      661 ctgctcgatg aaacgcgagg ccttcaacgc gctctcgatg ggctgcggcg ccacgtattt
      721 gccgccgctg gtcttgaaca tctccttctt gcggtcggtg atgtacagga agccgccctc
      781 cagcttgccg atgtcgcccg tgcggaacca gccatcggcc gagagcacct cgcgggtcag
      841 ttccggttcc ttgtagtaac cgatcatcac gttggggccc ttgatcagga tctcgccctc
      901 accttccggg tggcccggtt ccgggacgat gcgcacctcc accttgtcca acggacggcc
      961 cacgctcccg aatcgaaggc catcgttgcg cgcatcgttc acactcacca ccgggctcgt
     1021 ttcggtgagg ccgtagccct ccatcagcgg aattccagca gcgttgaaga tgcgggcgag
     1081 tcgcggttgc agggccgcgc tcccgctggc cacgcatgcg acctcgttgc cgagggcttc
     1141 gcgccatttg ctgaagacca gtttgctggc gatcccgagt tggagatcat accagaggct
     1201 ccggccgtgc acctcgtagc gctcgcccag gtccaacgac cagaagaaga gcttgcgctt
     1261 gatgccggtc agggcctcgc ccttggccag aatggtgtcg tagatcttct ccagcaggcg
     1321 gggtaccgcc gtgaacacgt gcggccgggc ctcgcagatg cgcgcgccca ggtcgtccaa
     1381 cgtctcttgg aagcgcactt gcgtacccgc tttctggtac aagtacatca gcatgcgctc
     1441 gtagatgtgg cacagcggca ggaagctgat cgcgcgtgca ccgggcttga cgggcagtct
     1501 gggcgcactg gcctccacgt tgctcaggat gttgtcgtgc gtgagcatca cccccttggg
     1561 cttgcctgtg gttccgctgg tataaatgat ggtcgccagg tcctcgcgct tcaccgcggc
     1621 cttgcacccg tccagcttcg cgcgctgcgt gatccccttg cccagcagct ctttccaatg
     1681 cgacgcgccg ggcaccttgt cgtacgtgaa gaggtgcgcc actgcgggga ccctgacctg
     1741 cgcggcgcgt gccttggtga acaggtcgtt gttgctcacg aagaacacct tggcacccga
     1801 atgctccagg atgtacgcgt agtcatccgc cgtcatggtg ggatagatgg gcacgctgat
     1861 ggcaccgatg cgcaggacgg cctggtccgt gatggcccat tccgcgcggt tgccactggc
     1921 cagggccacc ttgtctcccg gtgcgatccc caggtccagt aggcccaagg ccagttgctc
     1981 ggccgtatcg atgaattccc tggtgttgta cgtacggagc ttgccctcct ccagggaacc
     2041 cacacagacg tcctggggat attccgcgag ttggaagtcg gggatgtcga agagacgatg
     2101 gatggccatg tggatcggat ctcccggccg tcgccgccgg atgttggcgc aagatgggaa
     2161 aggaagcggt ccgtccggct gacattcgtc ctaccgtacg cccggctcgt ccgctatccc
     2221 gtgcgcagcg cgtccacgta cttcgtcacg tacgtgtcca actgttgcgc cctgtactgc
     2281 atgatgaaac gcgggtgttc caacggcgtg atgcgcgcga agaagccgtg ctccgcgttc
     2341 aacgtgttca ggaaggccag gttttttccc gtgccgatgc agaacacctc atcggtgcgc
     2401 atgccggtgg cgatgagccg gcgcagccac tgcaccacga aaggggtgat ggccttctgc
     2461 aacgccttgt cgtcgtagta gttcaggttc acctgcgtac cggtggagcc tgctttggtg
     2521 aagccgagcg ggcagatcgc ttgcacgtac acatcccggt aaaaggcatc ggccccgccc
     2581 gtgctgcgca ccatgcggta gaagaaatcg ctgctgggtt catgggtgcg catgccatgc
     2641 accggaatgc cgaggtcgct ttcgcagcgt ttggtgtcgg tgaaacagat gccggtgctg
     2701 cccgctccca agcgacccgg attgataccg agcatcagtt tgcgagggcg ctcgtcgttg
     2761 aagaagcgcc ggtggaagtc ccggacgatc cgcctt
//
LOCUS       DAOQOC010000303         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19783_length_2794_cov_3.153706, whole genome shotgun sequence.
ACCESSION   DAOQOC010000303 DAOQOC010000000
VERSION     DAOQOC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19783_length_2794_cov_3.153706"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PL106_05915"
     CDS             complement(<1..438)
                     /locus_tag="PL106_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNA32639.1"
                     /translation="MFRPLAQLTFRIWGWKVVDHRPQPMGQCIYLVAPHTSNWDFLLG
                     VCARSIGRLKNTKYLGKKELFRWPYGWLFRALGGYPVDRGKAANITEQVVGYFKTIPH
                     FSIGIAPEGTRKQVRSLKSGFWHIARGANVPLILTSFDYGRKEV"
     gene            627..1979
                     /locus_tag="PL106_05920"
     CDS             627..1979
                     /locus_tag="PL106_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNA32640.1"
                     /translation="MAVLTSLFFMWGFMTVMNDVLVPHLKRVFALDYSVGLLVQSFFF
                     GAYFTGSVLYYWMSRRQGDPIQRIGYKRGLIAGLVLSAVGSLLFVPATFLEVYGFYLF
                     AIFVLGLGFTLLQIAANPFVAIIGPPEGASSRLNLAQAFNSLGTTLAPLIGGALIFEL
                     LEGDDALRYPYLVFGLMLILQAVWVHFTALPEPAHRGTDARSNAWRHPHLRWGMLAIF
                     CYVGAEVAIGSLIINYLGLADVLGLTESGAKHYLSLYWGGAMTGRFLGAFALASNRGP
                     QWRMVSMALIAIAMCVLLFALNASARFNFAHFWPMAVLIGANMAAFMIAGRDPSRVLG
                     LFALAAAGLLAITIGSHGAWALWSVIAIGLFNSIMWSNIFTLAIDGLGDDTSQGSSLL
                     VMMIAGGALIPLLQGGIIDVMGRADRLALGFHWSFALPLLCYLYLAWYGFKGSRHARA
                     "
     gene            1976..>2794
                     /locus_tag="PL106_05925"
     CDS             1976..>2794
                     /locus_tag="PL106_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNA32641.1"
                     /translation="MILGIDIGGTTSKLGLVRDGQVIARARIGTTGHADDHAFADALA
                     HEARQLAGAHGTRISAIGIGAPNANQLTGIIEMAPNLPWKHDVPLARMMADRLGAPAV
                     LGNDANAAALGEWRYGAGQGINDLLVVTLGTGVGSGFIVNGQLVLGSAGYAGELGHTI
                     LIPGGRDCTCGRQGCLEAYVSIRGMIATYQECGGAPGAMDVKTIADRGHQGEQAAIDC
                     FRLTTDRLAIGLVNAVCATGPKRIVLFGGIARNGDLLMAPLRDRFHAMLLNIYKG"
BASE COUNT          436 a          902 c          906 g          550 t
ORIGIN      
        1 gacctccttg cgaccgtagt cgaaagaagt gaggatcaag ggcacgttgg caccgcgcgc
       61 gatgtgccag aatccgctct tgagcgagcg cacttgcttg cgcgtgccct cgggcgcgat
      121 gccgatgctg aagtgcggta tggtcttgaa gtagcccacc acctgttcgg tgatgttcgc
      181 cgccttgccc cgatccaccg gatagccgcc caaggcgcgg aagagccagc cgtacggcca
      241 acggaacaat tccttcttcc caaggtattt cgtgttcttg agtcgaccga tgctgcgggc
      301 gcacacaccc aacaggaagt cccagttgct ggtgtgtggg gccaccaggt agatgcactg
      361 gcccatgggc tgcgggcgat ggtcgacaac cttccagccc cagatgcgga aggtgagttg
      421 ggccaatgga cggaacattc ccggcttcag gttcgcgatg cgagggcgac aaacgtaggt
      481 cggagcggct gatggtgctc cttgggccgt ttcccggtcg cttagggtcc cggggctccc
      541 tatggatcgc aggccgtatc cttgtccatc atacaccaac cgcacgtttg acgcctccct
      601 cctcctctcg aacgaccacc cgctccatgg ccgtgctcac gtccctgttc ttcatgtggg
      661 gcttcatgac ggtgatgaac gacgtgctgg tgccgcactt gaagcgggtg ttcgccctgg
      721 actattcggt gggcctgctg gtacagtcat tcttcttcgg cgcctacttc accggttcgg
      781 tgctgtacta ctggatgtcg cgccgtcagg gcgaccccat ccagcgcatc ggctacaagc
      841 gcggcttgat cgcgggcctg gtgctgagcg cggtgggcag cctcctcttc gtgcccgcta
      901 ccttcctgga ggtgtacggc ttctacctct tcgcgatctt cgtgctaggg ctcggtttca
      961 cgttgttgca gatcgcggcc aatcctttcg tcgccatcat cggcccgccg gaaggagcca
     1021 gtagccgatt gaacctcgcg caggccttca actcgctggg aaccaccctg gccccgttga
     1081 tcggtggtgc gctcatcttc gaattgctgg aaggcgacga tgcgctgcgc tatccatacc
     1141 ttgttttcgg tctgatgctg atcctacagg ccgtgtgggt gcatttcacc gcgctgcccg
     1201 agccggccca ccgcggaacg gacgcgcgca gcaacgcctg gcggcacccg catctgcgct
     1261 ggggcatgct ggccatcttc tgttacgtag gggccgaagt ggcgatcggc tcgctcatca
     1321 tcaactacct ggggctggcc gatgtgctgg gcctgaccga gagcggggcg aaacactacc
     1381 tgagcctgta ctggggcggc gccatgacag gccgtttcct gggcgccttc gccctggcga
     1441 gcaaccgcgg cccacaatgg cgcatggtct ccatggccct gatcgcgatc gccatgtgcg
     1501 tgctcctgtt cgccctgaac gcgtcggccc ggttcaactt cgcgcatttc tggccgatgg
     1561 ccgtgctgat cggggcgaac atggcggcct tcatgatcgc ggggcgcgat ccgtcgcgcg
     1621 tgctggggct tttcgccctg gcggccgcag gcctgctggc gatcaccatc ggttcccacg
     1681 gcgcttgggc cctgtggtcg gtgatcgcca tcggcctgtt caactccatc atgtggagca
     1741 acatcttcac cttggccatc gacgggctgg gcgatgacac ctcgcagggc tccagcctgc
     1801 tggtgatgat gatcgcgggg ggtgccctca tcccgctgtt gcagggcggc atcatcgatg
     1861 tgatgggacg tgcggaccgg ttggcgctcg gcttccactg gagcttcgcg ctcccgttgt
     1921 tgtgctacct gtacctggct tggtacggct tcaagggatc ccggcacgca cgcgcatgat
     1981 cctgggcatc gatatcggcg gcaccaccag caaactcggt ctggtgcgcg atggccaggt
     2041 gatcgcccga gcccgcatag gcaccacggg ccatgccgat gaccacgcct tcgcggacgc
     2101 gctggcgcat gaggcgcgcc aactggccgg tgcgcacggg acccggatca gcgccatcgg
     2161 tatcggcgca ccgaatgcca accagcttac ggggatcatt gaaatggcgc ccaacctgcc
     2221 ctggaagcac gatgtgcccc tggcgcgcat gatggccgac cgactgggag cacctgccgt
     2281 gctgggcaac gatgccaatg ccgctgccct gggtgaatgg cgctacggcg cgggacaggg
     2341 catcaacgac ctcctggtgg tgaccctggg caccggtgtg ggcagcggct tcatcgtgaa
     2401 cgggcaactg gtgctgggca gcgcgggcta tgcgggtgaa ctggggcaca ccatcctgat
     2461 cccgggaggc cgggattgta cctgcgggcg gcagggctgc ctggaggcct acgtgagcat
     2521 ccgcggcatg atcgccacct accaggaatg cgggggggcg ccgggcgcca tggacgtgaa
     2581 gaccatcgcc gatcgtggcc accagggcga acaggccgcg atcgactgtt tccgcctcac
     2641 caccgaccgg ctggccatcg gcctcgtgaa tgccgtgtgt gccaccggtc ccaagcgcat
     2701 cgttctcttc ggcgggatcg cacgcaacgg cgatctgctc atggcgccgc tgcgcgatcg
     2761 cttccacgcc atgctgctga acatctacaa gggg
//
LOCUS       DAOQOC010000304         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19863_length_2788_cov_3.282108, whole genome shotgun sequence.
ACCESSION   DAOQOC010000304 DAOQOC010000000
VERSION     DAOQOC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19863_length_2788_cov_3.282108"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PL106_05930"
     CDS             <1..533
                     /locus_tag="PL106_05930"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA32642.1"
                     /translation="SFICVGTAGWLALQAFRQNALDYLEKPIDPEDLRRAVRKLIRIV
                     GDPDDGAHHHASLDALINDPDSPLSKRVAVPGRDGLVLIRHEDITHLEASNSYTTVFT
                     NDGRKVISSKNIRVFETHLDAKRFFRVHKSYIINLEHLKGFSRGEGNMALLDNGALIP
                     VSRRRLPDLLGMIETF"
     gene            726..>2788
                     /locus_tag="PL106_05935"
     CDS             726..>2788
                     /locus_tag="PL106_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA32643.1"
                     /translation="MAFLCISTTAQQFGFTQYTPKDGLAQSQARSIAQDVDGYLWFGT
                     LGGVSRFDGHTFRNFALQEGLPDAQVNAVLCAKDGTVWLGAGGSLMTFEGRRTTVVPL
                     PGVARDARVLCLAEDKEGAILVGTDGAGLFRSAKGVVVPLGGFPSDTASTVRALLALP
                     DGSIIIGTRSGAYRWKDNDCAAIPLGDGPRSISALAAGTDGTLWFGTFGQGLFGLKPD
                     GELVEYDEESGLVQNNIRALLADRNGRLWVGTKFGVNTLDGGRLRSYTVHQGLPNDNI
                     WCLFQDAEGGIWLGTDGAGVLRWAGDRFVTWTVTDGLCSDLVMALCADARGDIWLGTY
                     GNGVCRLDNMAAITTLDGLPNNTVWSATSASDGSLWFGTTEGLVHIVNGRVVPPEPGR
                     DLGAVRVLALHMDDDGTLWCGTREGLAILPPPGGGPLRTMARDERTLLRSVRAIVRAG
                     DAHLLATDLGLVRVSGERTERWTTADGLCDNTVQCLLLDNKGRTWIGTSNGLSCRVGE
                     HFTNIRLGDDFGSNFIDLLLADNVGHVWAGTNNGLFRFHPDSLLADPSQKEQFTIVDG
                     VRGTECNLNAGFLDREGRLFFGTNAGLVMHDPARGPATRPESPATARITGLRSFMLPT
                     NWKGKCDSLDIRTGLPIGLRLDYRKNHLTFDYTAVALADADHVRFQCRLLGFDTEWLP
                     PTDAR"
BASE COUNT          458 a          919 c          899 g          512 t
ORIGIN      
        1 tctccttcat ctgcgtcggg accgccggct ggctcgccct gcaggccttc cgccagaacg
       61 ccttggacta cctggagaag ccgatcgacc cggaggacct caggcgcgcg gtgcggaaac
      121 tcatccgtat agttggcgac ccggacgatg gggcccatca tcacgcttca cttgatgcgc
      181 tcatcaatga tcccgattcg ccgctcagca aacgggtggc ggtacccggc cgcgatggac
      241 tggtgctcat ccgccacgag gacatcaccc acctggaggc cagtaacagc tacaccaccg
      301 tcttcacgaa cgatggccgg aaggtgatca gcagcaagaa catccgcgtg ttcgaaacgc
      361 atctcgacgc caagcggttc tttcgcgtcc acaagagcta catcatcaac ctggaacacc
      421 tcaaggggtt cagccggggc gagggcaaca tggcgctgct ggacaacggg gcgctgatcc
      481 cggtgagccg tcgtcgtttg cccgacctgc tgggtatgat cgagactttt tgaacctccc
      541 gttcccggag aatgcccctc atccccgccg gatccccgcg atcacatcac ggtacgccag
      601 ggaacatccc tggaccgcat ggagggccgg tccgatgcgc tcggccggga gccttgtcca
      661 cgtggagatc ttggctgtcc gcagccctgt tccggatccc gttgcgcgcc ctgctcgttc
      721 cggcgatggc gttcctctgc ataagtacca ccgcccagca gttcggcttc acccagtaca
      781 cgcccaagga tggcctcgcg caaagccagg cgcgcagtat cgcgcaggac gtggacggat
      841 acctgtggtt cggtacactc ggcggggtca gccgctttga tgggcacacc ttccgcaact
      901 tcgccttgca ggaggggctg cccgatgcgc aggtgaacgc cgtgctgtgc gccaaggacg
      961 gtacggtgtg gctgggggcg ggcggttcgc tgatgacctt cgagggtcgg cgcacgaccg
     1021 tggtgccgct gcccggggtg gcgcgtgatg cccgcgtact atgcttggcc gaggacaagg
     1081 agggcgcgat cctcgtcggg accgatggcg cggggctgtt ccgcagtgcc aagggagtgg
     1141 tggtcccgct gggcgggttc ccttccgata cggccagtac ggtgcgcgcg ctgctggccc
     1201 tgcccgatgg ctccatcatc atcggcacgc gctccggcgc ctaccgctgg aaagacaacg
     1261 actgcgcggc gatcccgttg ggtgatggtc cgcgaagcat cagtgcgctc gctgccggca
     1321 cggacggcac gctgtggttc ggcaccttcg gccagggcct cttcggcctg aagcccgacg
     1381 gcgaactggt cgaatacgac gaggagtccg gtctggtgca gaacaacatc cgcgcgctgc
     1441 tcgctgaccg caacggccgg ctgtgggtcg ggaccaaatt cggtgtgaac accctggacg
     1501 gtggccgcct gcgctcctat accgttcatc agggattgcc caacgacaac atctggtgcc
     1561 tgttccagga cgccgagggg ggcatctggc tgggcaccga tggtgcgggc gtgctgcgct
     1621 gggccgggga ccgctttgtt acctggaccg tgaccgacgg cctctgcagc gacctggtga
     1681 tggcgctctg cgccgatgcg cgcggcgaca tctggctggg tacgtacggc aacggcgtgt
     1741 gccgactgga caacatggcc gcgatcacca cgctggatgg cctgcccaac aacaccgtgt
     1801 ggagcgccac ctccgccagc gatggatccc tgtggtttgg tactaccgaa ggtttggtcc
     1861 atatcgtcaa tggtcgtgtc gttccgccgg agcccggtcg cgacctcggt gcggttcgtg
     1921 tgctcgcgct gcacatggat gatgatggca cgctctggtg cggcacgcgc gaagggctcg
     1981 ccatccttcc cccgcctggt ggcggcccct tgcgcaccat ggcccgtgat gagcggaccc
     2041 tgctgcggtc cgtgcgtgcc atcgttcggg cgggtgatgc ccacctcctg gccaccgacc
     2101 ttggtctggt gcgtgtgagc ggggagcgta ccgagcgctg gaccaccgcc gatggcctgt
     2161 gtgacaacac cgtgcaatgc ctcttgctgg acaacaaggg ccgcacatgg atcggcacca
     2221 gcaacgggct cagctgccgt gttggggagc acttcaccaa tatccgcttg ggcgatgact
     2281 tcggctccaa cttcatcgac ctgttgctgg ccgacaatgt tggccatgtg tgggcgggca
     2341 cgaacaacgg actgttccgt ttccatccgg acagcctgct ggctgatccc tcgcaaaagg
     2401 aacagttcac gatcgttgac ggagttcgcg gtaccgaatg caacctgaac gccggcttcc
     2461 tcgaccggga gggccgtctc tttttcggca ccaatgcggg cctggtgatg cacgaccccg
     2521 cacgggggcc cgccacccgg ccggaaagcc cggccactgc ccggatcacc ggtctgcgct
     2581 ccttcatgct gcccaccaac tggaagggca agtgcgacag cctggacatc cgtacagggc
     2641 tgcccatcgg cctgagactc gattaccgga agaaccacct caccttcgac tacaccgccg
     2701 tggccctggc cgatgcggat catgtgcgtt tccagtgccg cctcctgggc ttcgataccg
     2761 aatggctgcc tcccaccgat gcgcgttt
//
LOCUS       DAOQOC010000305         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19923_length_2784_cov_3.474166, whole genome shotgun sequence.
ACCESSION   DAOQOC010000305 DAOQOC010000000
VERSION     DAOQOC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19923_length_2784_cov_3.474166"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PL106_05940"
     CDS             complement(<1..1099)
                     /locus_tag="PL106_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32644.1"
                     /translation="MKRCCLLLAVINFALGVCAQNPHVIQAEWFIGPDPGEGLGTAMQ
                     VADGAWDEAMEAVIATLPPQAPGSTTIGVRVKGANGYWSSAFTSVIHTGAALTARSVN
                     VQQGEYFWDTDPGEGAGTPLLAFDGDFNDALEQAMASDNSITTGAHRLYVRVRGADTG
                     WSALFTQVVQVNTPLSARDIHVQAGEFFFDSDPGEGNATALLAFDGDWNAAVETGMAS
                     VPSPAVGDHLLYIRMRSADGQWSNEYKTVLHVGPQLTPRPVMVQAAEYFWGTDPGEGA
                     GLPMLAFDGDFNSAIEQADIASNGATLGANVLGVRVLGADGGWSTTFSSIVHVGPALA
                     LRDVRIQFGEYFYDVDPGAGNGTALTAFNGAW"
     gene            complement(1111..2229)
                     /locus_tag="PL106_05945"
     CDS             complement(1111..2229)
                     /locus_tag="PL106_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32645.1"
                     /translation="MKRNIHLLLAVATASSIQATDRVVSPNGTYNTISSAIVASSDGD
                     RILVEPANYTEDVLLGKSLSILCNQEGGRYKLNGSLTLVAPSGKDILISGIRVMNGVL
                     VSGTTTAPGTFRMVDSYASHCNLEDPYLHVELYRDTVAVSIFFSAGTVAGNNIPGTSS
                     GGGAIVLSGGSQLVEESWIIGNAISYAYNGWGVSITTDAVFHVENNFIRKFPGAVGMT
                     ISRSQEPAASISTIVNNTFYMPSGAPTTCINNANLSRFNLLVKNNVMVGYNAVISQDN
                     GWTQLVGSHNLFAAPSWIDLTTGQSLAATPLIDAGDPDPRYLDLDLTTNDVGCYGGSN
                     SRANFTTPMGSAVVGFMNAPRVVAQGETVNISATGFDR"
     gene            complement(2243..>2784)
                     /locus_tag="PL106_05950"
     CDS             complement(2243..>2784)
                     /locus_tag="PL106_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32646.1"
                     /translation="YSFTATAPDMTVGGAPHGNMDIVMELFSGACGLLTSVACGDVGG
                     SGAADDMIATGLTVGETYHFRVYDFRTQFAFEEPGYDLCVVEGVGSGVGVDETVDLPG
                     AALYPNPTTGLFTIQVGAANGPFSLDVFDTAGRKVLSTTRNISSGMVQVDASSLKPGA
                     YVARFNNGTRVVNERLIIQ"
BASE COUNT          540 a          864 c          828 g          552 t
ORIGIN      
        1 cccatgcgcc attgaacgcc gtgagtgcgg tgccattgcc cgctccaggg tcgacatcat
       61 agaagtactc cccgaactgg atgcgcacat cgcgcaaggc gagagctggc cctacatgca
      121 cgatgctgct gaacgtagtg ctccatccgc catccgctcc gagcacacgc acgccgagca
      181 cattggctcc caaggtggct ccgttcgagg cgatgtcagc ctgctcgatc gcactgttga
      241 agtcgccatc gaaggccagc atcggcaggc ccgcaccttc tccagggtct gttccccaga
      301 agtactcggc cgcctgcacc atgaccggtc gcggcgtgag ctgtggacca acatgcagca
      361 cggtcttgta ctcgttgctc cactgcccgt ccgccgagcg catgcggatg tacagcaggt
      421 gatcgcccac ggcaggtgac ggtacgctgg ccatacccgt ctccacggcc gcgttccaat
      481 cgccatcgaa ggccagcaac gccgtggcgt tcccctcgcc cggatcgctg tcgaagaaga
      541 actcccccgc ctgcacatgt atatcgcgtg cgctcagcgg agtattcacc tgcaccactt
      601 gggtgaagag cgcgctccat ccggtatcgg cacctcggac ccggacatac aaacgatgcg
      661 cgccagtggt gatcgaattg tctgaggcca tggcctgctc cagcgcatcg ttgaagtcgc
      721 catcaaaggc cagcaacggt gtaccagctc cctcgccggg atccgtatcc cagaaatact
      781 cgccttgctg cacgttcacc gatcgcgcgg tcagggcagc accggtatgg atcacggatg
      841 tgaaagcact gctccaatag ccgttcgcgc cctttacccg cacaccgatc gttgtgcttc
      901 ctggtgcctg ggggggcagc gttgcgatca ccgcttccat cgcttcatcc catgcaccgt
      961 cggccacttg catggcggtt ccaaggcctt cgccaggatc ggggccgatg aaccattccg
     1021 cttggatcac gtgcgggttc tgtgcgcaca cgcccagagc gaaattgatg acagcgagaa
     1081 ggaggcaaca acgcttcatg gcatcaaggt tcaacggtcg aaaccggttg ctgagatgtt
     1141 caccgtttcg ccctgcgcca ccacgcgcgg tgcgttcatg aagcccacta cggcgctgcc
     1201 catgggcgtg gtgaaattcg cccggctgtt gctgccgcca tagcagccta catcgttggt
     1261 ggtcaggtcg aggtcgaggt agcgcgggtc gggatcccct gcatcaatga ggggtgtggc
     1321 ggcgagcgac tgaccagtcg tcaaatcgat ccacgacggg gccgcgaaaa gattatggga
     1381 tccgaccaat tgggtccaac cattgtcctg actgatcaca gcgttgtatc cgaccatcac
     1441 attgttcttc accagcagat tgaatcgact gaggttggcg ttgttgatgc aagtggtcgg
     1501 tgcgcccgaa ggcatgtaga aggtgttgtt gacgatggtc gagatggagg ccgcaggttc
     1561 ctgcgatcgg ctgatggtca tgcccacggc accggggaac ttccggatga aattattctc
     1621 tacgtggaag accgcatcgg tggtgatgct cacgccccaa ccattgtatg cgtagctgat
     1681 cgcgttgccg atgatccagg actcctcgac cagctgcgat ccaccgctga gcacgatggc
     1741 tccgccaccg ctggaggttc cgggaatgtt gttgcccgct acggtaccag ctgagaagaa
     1801 gatcgaaacg gccaccgtat cccgatacag ttccacatga aggtacgggt cttccaggtt
     1861 gcagtgcgaa gcgtatgagt cgaccatgcg gaaagtccct ggagcggttg tagtaccgct
     1921 aacgagaacg ccgttcatca cccgtatccc ggagatcaag atatccttcc cgctgggtgc
     1981 caccagggtg aggctgccgt tcaacttgta gcgcccaccc tcctggttgc acaggatcga
     2041 caggctcttg ccgagaagca cgtcttcggt gtagttggca ggctccacca gtatccgatc
     2101 cccatcactg ctcgcgacaa tggcgctgct gatggtgttg tacgtaccgt tggggctcac
     2161 cacgcggtcg gtggcttgga tgctcgacgc agtagcaaca gcgagcagca ggtggatgtt
     2221 ccttttcatg gtctgtcagg atctattgaa tgatcaagcg ttcgttcacc acgcgggtgc
     2281 cgttgttgaa gcgtgccacg tatgcaccgg gtttcaaaga agaagcatcc acctgcacca
     2341 tgccactgct gatgttgcgc gtggtgctca ggaccttccg tccggccgtg tcgaacacat
     2401 cgagcgagaa cggcccgttc gcagcgccga cctggatggt gaataggccg gtggtggggt
     2461 tcgggtacag ggcggcaccg ggcaggtcga ccgtttcatc aacccccacc ccgctgccca
     2521 cgccctccac cacgcacagg tcgtagcccg gttcttcaaa ggcgaactgg gtgcggaagt
     2581 cgtacacgcg gaagtggtag gtctcgccca ccgtgaggcc cgtggcgatc atgtcatcgg
     2641 ctgcacccga tccgcccaca tccccgcaag cgacggaggt gagtaaaccg caggcaccgg
     2701 agaacagttc catcacgatg tccatgttgc cgtgcggggc accgcccacg gtcatatccg
     2761 gcgctgtggc ggtgaagctg tacc
//
LOCUS       DAOQOC010000306         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_19929_length_2784_cov_2.805790, whole genome shotgun sequence.
ACCESSION   DAOQOC010000306 DAOQOC010000000
VERSION     DAOQOC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19929_length_2784_cov_2.805790"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..920)
                     /locus_tag="PL106_05955"
     CDS             complement(150..920)
                     /locus_tag="PL106_05955"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA32647.1"
                     /translation="MSTDLSYHFNGKVALITGAASGIGLATAKRFAQAGAAVVMAGND
                     MEHVQRAAGEVGANGANVLPVQCDVAVEAQVQAMVEATVKAFGRLDFAYNNAGIMSRN
                     TETSDLTAEEWERVIQINLTGIRNCMKHQLLHMKKQGSGAIINCSSMGGLKGAKGLSA
                     YSASKFGVLGLTRSAALEYAAQGIRINAVCPGFVHTPMERIVSAGSDEVLAAMAQMVP
                     AGRFCEPEEIADAVLWLCSSGSTMVIGQALAVDGGITT"
     gene            complement(1254..2273)
                     /locus_tag="PL106_05960"
     CDS             complement(1254..2273)
                     /locus_tag="PL106_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNA32648.1"
                     /translation="MKATIMHGPGDVRYETVPDPRIEKPTDALIKLSATCICGSDLWP
                     YRGLNDSKGPQHMGHEYCGVVVEVGSAVRTVKPGQFVVGSFCISDNTCPHCAYGFHSS
                     CEQIEFMTGAQAPYARVPLADGTLVATAEMPDADMIPHMLAASDVLGTGWYAAVAAQV
                     RKGGTAVVVGDGAVGLMGVLAAKELGAERIIAMSRHAPRQALAREFGATDIVAERGAE
                     GIARVKELTGGVGAESVLECVGMAASMEQAMGVCRPGGTIGYVGVPHGVTLDGQKLFF
                     AQHRMLGGPAPVRRFLPDLMDRVLKGSIQPGKVFDLTLPLAQVAEGYKAMDERRAIKT
                     LLKVD"
     gene            complement(2342..2776)
                     /locus_tag="PL106_05965"
     CDS             complement(2342..2776)
                     /locus_tag="PL106_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNA32649.1"
                     /translation="MIALVRPVPVQTSAFPVGAENSGYAQYFSGRSYLAPLTTDERLK
                     VPLSNVTFEPGCRNNWHKHSGGQLLIVVGGVGYYQERGKPARRLVPGDVVEIPPGTEH
                     WHGAAPDSWFSHLATAGSPDTNKNTWLEPVSDADYRAATGGQ"
BASE COUNT          432 a          935 c          902 g          515 t
ORIGIN      
        1 aggaaggtgg tgatcatggt gtttggtttg tcgaaaggta agtgcggcac ctccagggtg
       61 gtgcggaaca cccaggcaac cggaaatgga tccgtggtca aacaacggtt ccgacccctc
      121 tgggaagtgt aaccgcataa gcgatcctct cacgtggtga tccccccatc aacagccagc
      181 gcctggccga tcaccatggt ggagccggag ctgcagagcc acagcacggc gtcggcgatc
      241 tcctccggtt cgcagaagcg gccagccggt accatctggg ccatggctgc aagcacctca
      301 tcgctcccgg ccgatacgat gcgctccatg ggcgtgtgta cgaagccggg gcacaccgca
      361 ttgatgcgga tcccctgcgc ggcgtattcc aacgcggccg accgcgtgag cccgagcacg
      421 ccgaacttgg aggcggagta ggccgagagg cccttcgcac ctttcaggcc gcccatggag
      481 gaacagttga tgatggcgcc actgccttgc ttcttcatgt gcagcaactg gtgcttcatg
      541 cagttgcgga tgcccgtgag gttgatctgg atcacacgct cccactcctc cgccgtgagg
      601 tcgctcgtct ccgtgttgcg gctcatgatg ccggcgttat tgtaggcgaa gtccaggcgc
      661 ccgaaggcct tcaccgtcgc ctccaccatc gcctgcacct gcgcttccac cgccacgtcg
      721 cactgcacgg gcagcacatt ggccccgttc gcgcccacct ccccggccgc ccgttgaacg
      781 tgttccatgt cgttcccggc catcaccacc gcagcgccgg cttgggcgaa ccgtttggcc
      841 gtggccagcc cgataccgga tgccgcgccg gtgatcaagg ccaccttgcc gttgaagtgg
      901 taggataggt cggtgctcat gggtgtggat gttgaagttg gttgtatgtc gtgccagcgt
      961 tcgggagggc cttccgccta cgacctcggc aacatcccga aggtagagga acacatgccc
     1021 tggtggtcgc gtcggggttg aagagatcca tccacctggc gatcaacccc tgctcgcccc
     1081 cggagatccg tgcgcttggt acccggaagc gcgcgagcgc ccggcccacc ggctttccat
     1141 gcacgtgtgt tggccgtggc tgatgcgccg cgcgatgacc aggccgatgc cgcccgcgcc
     1201 gatcacttcg gtcagtcgtt catgtcctca acgctttgga aggtacgttt tgttcaatcc
     1261 accttcaaca gcgtcttgat cgccctgcgt tcgtccatgg ccttgtagcc ttcggccact
     1321 tgggcaaggg gcagggtgag gtcgaacacc ttgccgggct ggatgctgcc cttgagtacg
     1381 cggtccatca ggtcgggcag gaagcggcgc acgggcgcgg ggccgcccag catgcggtgc
     1441 tgcgcgaaga acagcttctg gccgtcgagc gttacgccgt gcggcacgcc tacgtagccg
     1501 atggtgcctc cggggcggca cacgcccatg gcctgttcca tggaggcggc catgcccacg
     1561 cactccagca cgctctccgc gccaacgccg ccggtgagct ccttcacccg ggcgatgcct
     1621 tccgccccgc gctcggccac gatgtccgtg gcaccgaact cgcgcgccag ggcttggcgg
     1681 ggagcatggc ggctcatggc gatgatgcgt tccgcgccca gctccttggc ggccagtacg
     1741 cccatgagcc ccaccgcgcc atcgcccacc accacggcgg tgccgccctt gcgcacctgc
     1801 gcggccacgg ccgcgtacca gcccgtgccc agcacgtccg atgccgccag catgtgcggg
     1861 atcatgtccg catctggcat ttccgccgtg gccaccaggg tgccatcggc cagcggcacg
     1921 cgggcgtaag gcgcttgcgc accggtcatg aattcgatct gctcgcacga cgaatggaag
     1981 ccatacgcgc aatgcgggca ggtgttgtcc gatatgcaga acgagcccac cacgaactgg
     2041 ccgggcttca ccgtgcgcac ggcggaaccc acctccacca ccacgccgca gtactcatgg
     2101 cccatgtgct gcgggccttt gctgtcgttg aggccgcggt agggccacag gtccgagccg
     2161 cagatgcagg tcgccgacag tttgatgagg gcgtcggtgg gcttctcgat ccgcgggtcg
     2221 gggaccgttt cgtaccggac atcgccgggg ccgtgcatga tggtggcttt catggagttg
     2281 tgctcgttgg gttggttcgc tgttctgaga tcgatgggtg ctgcgctgcc ggagcttccg
     2341 gttactgccc gccggtggcc gcccggtaat cagcgtcgct gaccggttcg agccaagtgt
     2401 tcttgttcgt gtccgggctg cccgccgtgg cgagatggga gaaccaactg tcgggggccg
     2461 caccgtgcca gtgctcggtc ccgggcggta tttccaccac atcgccgggc accagccgcc
     2521 gtgcgggctt accgcgttcc tggtagtagc ccacgccgcc caccacgatc agcaactgcc
     2581 caccgctgtg cttgtgccag ttgttgcggc agccgggctc gaaggtcacg ttggagagcg
     2641 ggaccttgag ccgttcgtcg gtggtcaacg gggccaggta ggaccgcccg ctgaagtact
     2701 gggcgtatcc cgaattctcc gcgcctaccg ggaaggcgct cgtttgcacc ggcaccggcc
     2761 ggaccagcgc gatgatcgcg tcga
//
LOCUS       DAOQOC010000307         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20153_length_2767_cov_3.128319, whole genome shotgun sequence.
ACCESSION   DAOQOC010000307 DAOQOC010000000
VERSION     DAOQOC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20153_length_2767_cov_3.128319"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..926
                     /locus_tag="PL106_05970"
     CDS             210..926
                     /locus_tag="PL106_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32650.1"
                     /translation="MNWKIALLVAVITGVVTAIVTAPVANHVAGKVGVSDFEGKRGFA
                     VAFLFIPAGFLGGALLGLLCTKLVHAAEWPDFWKALGLSVALGQAALFGISGSFLLGI
                     PRPPRIDGKELLLELDVYVPLARITAAAREPDQIRLSLYAGPKDNAYAMIDRSLFREE
                     DGQFIVTAVAPLNSRSYTRTVSFHIQDHTWLAADIELPATPGTEHMAWSALKPMRDAR
                     TSGSSSVMSDVRMRYRVVKP"
     gene            complement(942..2441)
                     /locus_tag="PL106_05975"
     CDS             complement(942..2441)
                     /locus_tag="PL106_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32651.1"
                     /translation="MMRSVTIIEQGREGRVRYAGPIGSLEGYLEFGGNDVVANVNMGS
                     RVDRARSHPWAVEQWTEVLRFIADEVIRQRAPACTAVIDEQRGEIILRRSGVSAPPPV
                     PTSPTPQQKSVAFVRRYSTLKAMVGIGLLVITIVGGAVFWIGKKTLMITALSGIPLNE
                     CVRTDQHIASLIQTTDPHAVEISGRGANTTTSVSIILIPLNGSKPQLVPVATGLNGNS
                     LGLSRIMGSDGRTLWFDAAGLNGVRLSDHELITPKDLRAANPALDPSWWEDPRGMDLV
                     DGRLHIMRDDRSAAIELDPASLKASPTTPKPSNRYFDRHSPADFLASGSIRTPGTWLG
                     LLSEQELGSEYQPGKRVGPVEHANSAKVLRRLYKAEIEPATDGTHHRIRSIAPLDSTT
                     YLNGAFVRMDVRSEPIRLNDPDGLLMLYTSAPGQQGTATVARIDDQGRILWKVDTGID
                     RFKLEQILPGPGSTAFVGPRPPIPGKLSEPLVVIVEHGTGKVATHSLWR"
     gene            complement(2460..>2767)
                     /locus_tag="PL106_05980"
     CDS             complement(2460..>2767)
                     /locus_tag="PL106_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNA32652.1"
                     /translation="NPPAMTPEVFEKFLPYAIAFGVEEVWGDRFQDMINKALVDPNYR
                     SGWYSGSIPHYGAFSRSMSSSLNSSVSSSSTPPSKSGGSGGGGSSGGGGGGGGGGGG"
BASE COUNT          479 a          885 c          865 g          538 t
ORIGIN      
        1 ttacgacacg gtggatacga aggccatgaa gcggatcgtg accgaactga gagccggcaa
       61 ggtgaagcga tgagaggcga gaccgtggcg cagggcgctt gcccgccgta ggcaggacga
      121 ccctagttca tgggagatgt cccggatccg atccgtgctc aggcatcggt cgtgagtttc
      181 aaggtcctgg aatgaccacc ttcgccaaca tgaattggaa gatcgctctc cttgttgccg
      241 tgatcaccgg tgtggtcacg gccattgtaa cggcacctgt cgctaaccac gtcgcaggaa
      301 aggtcggcgt gagcgatttc gagggcaagc gcggattcgc cgtcgctttc cttttcattc
      361 cggcgggttt cctcggcggg gcgctgctgg ggctgctctg caccaaactc gtgcatgccg
      421 cggaatggcc ggacttctgg aaagccttgg gcctttcggt ggccttgggc caggcagcac
      481 ttttcggtat ttcaggatca ttcctgctgg gcattcctcg gccgccgcgc atcgatggca
      541 aggaactgtt gctggagctc gatgtatacg tacccctggc gcggatcacc gctgccgcgc
      601 gcgaaccgga ccagatccgc ctcagcctgt atgccgggcc gaaggacaat gcctacgcca
      661 tgatcgaccg gtcgctcttc cgagaggaag atggccagtt catcgtcacc gccgtcgctc
      721 cgctcaactc caggtcatac acgcggaccg tgagcttcca catccaagac cacacctggc
      781 tggcggcgga catcgaactg cccgccacgc ccggaacgga acacatggcg tggagcgcgt
      841 tgaagccgat gcgcgatgcg cgaacctcgg gtagcagcag tgtaatgagc gatgtgcgga
      901 tgcgttatcg agtggtgaag ccctgaggtg cttctgccgc gctagcgcca cagggaatgc
      961 gtggcgacct taccggtgcc gtgctccacg atcactacaa gcggctcgct caacttgccg
     1021 ggtatcggcg ggcggggacc tacgaacgcc gttgatcccg gccccggcag gatctgctcc
     1081 agcttgaacc ggtcgatccc ggtatccacc ttccacagga tccggccttg gtcatcgatc
     1141 cgtgccaccg tggctgttcc ctgttgaccc ggggcggacg tgtagagcat cagtagtccg
     1201 tccggatcgt tcagtcggat gggttccgac ctcacatcca tgcgcacgaa ggccccgttc
     1261 aaataggtgg tgctgtccaa cggtgcaatg gaacggatcc ggtggtgtgt accatccgtt
     1321 gcgggttcga tctcggcctt gtacaggcgt cgcagcacct tcgcgctgtt ggcatgctcc
     1381 accggcccta cccgcttgcc gggttggtac tcgctaccaa gctcttgctc cgacagcagc
     1441 ccgagccaag tgcccggtgt acggatgctg cccgatgcga ggaagtccgc tggcgaatgc
     1501 cgatcgaagt acctgttgga aggtttgggg gtcgtgggtg aagccttcaa ggacgcaggg
     1561 tccagctcga tggcggcgct gcggtcatcg cgcatgatgt gcaaccggcc atccacgagg
     1621 tccataccgc gcgggtcctc ccaccaagag gggtccaatg cggggttggc cgcgcgcagg
     1681 tccttcgggg tgatcagctc gtggtcgctc aagcgcaccc cgttcagccc ggcggcatcg
     1741 aaccagagcg tacgcccatc gctgcccatg atgcgcgaga ggcccaggga gttcccgttc
     1801 aagccggtgg ccacaggtac cagttggggc ttggacccgt tcagcgggat caatatgatg
     1861 ctgacgctcg tggtcgtgtt ggcgccccgg ccgctgatct ccaccgcatg cgggtccgtg
     1921 gtctggatca gtgaggcgat atgctggtcc gttcgcacgc actcgttcag cggtatcccg
     1981 ctcaacgccg tgatcatgag cgtcttcttc ccgatccaga agaccgcacc cccgacaatg
     2041 gtgatgacga gcaggcctat ccctaccatg gctttgaggg ttgaataccg gcggacgaag
     2101 gccacgctct tctgttgcgg cgtgggcgat gtgggcacgg gcgggggggc gctaacacct
     2161 gaccttcgta ggatgatctc gccgcgctgc tcatcgatca cggctgtgca tgcaggtgct
     2221 cgttgcctga tcacttcatc ggctatgaaa cgcaggactt ccgtccactg ctccacggcc
     2281 catggatggg agcgcgcccg atccacacgc gagcccatgt tcacgttcgc cacgacgtcg
     2341 ttgccaccga attccaggta gccttccaac gagccgatcg gtccggcgta ccgcacgcgg
     2401 ccctcgcgcc cttgctcgat gatcgtaacg ctgcgcatca ttcctgccat gcatacggct
     2461 taccccccac ctcctcctcc gccaccgcca ccgccacccg aagaaccccc gcccccactt
     2521 ccaccgctct tgctgggcgg cgtgctgctg gaactgacgc tgctgttgag cgaggacgac
     2581 atgcttcggg agaacgcgcc gtagtgcggg atggagccgc tgtaccaacc ggaccggtag
     2641 ttcgggtcca ccagcgcctt gttgatcatg tcctggaagc ggtcgcccca cacctcctcc
     2701 accccgaagg cgatggcgta gggcaggaac ttctcgaaga cctcgggcgt catcgcgggc
     2761 gggttga
//
LOCUS       DAOQOC010000308         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20202_length_2764_cov_3.456257, whole genome shotgun sequence.
ACCESSION   DAOQOC010000308 DAOQOC010000000
VERSION     DAOQOC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20202_length_2764_cov_3.456257"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PL106_05985"
     CDS             <1..601
                     /locus_tag="PL106_05985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA32653.1"
                     /translation="VERAKAEKHRRILFAGNGFYRKGGIELLKAFQRLGRTDARLWIV
                     STLEVDWGVFPTREDIAWAERTIADDPRITLHRGLPHADLLQLMRKAHVFTSTTFSDP
                     FNNTVLEAMGAQLPVISSDVSSIPEIVAHERNGWLLPVTDRPSDEIADDIAERLKQLM
                     EDPALYRRMSAESLAVTREKFDLRVRNERLLAIYEQALA"
     gene            complement(609..1385)
                     /locus_tag="PL106_05990"
     CDS             complement(609..1385)
                     /locus_tag="PL106_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32654.1"
                     /translation="MRELLLLFATCAVAMLHGQVRSDRAIVLEGADPADRAVRGLKDP
                     VTADEAMNAGAIQGGGYLYAEVSGDGWQAVLQPAVPSPVAGLRILLHVANGNTGPVTL
                     SVNGSAPIPVLKNGDQPLSPGDVQSGATASVVFDGTAFQLIDARRIERKPCPDGTVAV
                     NAMYCIETAQHDSIDFPEAVNVCGALGMRICSWAQFYLACYNAGSLGITDLTGDWEWT
                     DDTGNSPGQLRVVGQSSCTQASVGTGWDVQARYFHCCFRR"
     gene            complement(1388..2164)
                     /locus_tag="PL106_05995"
     CDS             complement(1388..2164)
                     /locus_tag="PL106_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32655.1"
                     /translation="MKRALLPCCLITCATYAQIHVDRPVVLTATDPAERTVQGSAPAL
                     EADGLITLDGARSGRYHWGQASGMGMAIQLALTPPAEAYVNGMELRFVPTAPGMGPVS
                     VNVDGLGARRIYRSDGLPVSAGLLVPGTVATLVYADTAFFTAARAPATCPDGFMPVNG
                     QYCIQRNDTVNVSFFTATRWCNDRGARLCSYGEYIHACTMHQAELEGLFNDWEWIDDT
                     SDHTHMADIAGRWVCHSISNLAMIESNNNYARVRCCLTLK"
     gene            complement(2161..>2764)
                     /locus_tag="PL106_06000"
     CDS             complement(2161..>2764)
                     /locus_tag="PL106_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32656.1"
                     /translation="NATTFATVSGTDELSATLTPALVTYTPGLRLTLLPTSTNNSGAT
                     LNVNGLGAVALRKNINMPLDSGDLRPGIPVHVIHDGAVFQLTGQLYPGCPAGFAAANA
                     DVCIEMAPSDTINWYSAVIRCGNAGTRLCSVQEWLQGCLSIPGFQATIANYEWVDSAA
                     NYNNMAKLMGWSDTSTAGDCHLGSRQTPLTKFRSRCCYDR"
BASE COUNT          506 a          868 c          883 g          507 t
ORIGIN      
        1 ggtcgagcgg gcgaaggccg agaagcaccg ccgcatcctc ttcgccggca acgggttcta
       61 ccgtaaagga ggcattgaat tgctgaaggc cttccagcgc cttgggcgaa cggatgcccg
      121 gctctggatc gtgagcaccc tggaggtgga ttggggcgtc ttcccgacca gggaggacat
      181 cgcatgggcc gagcgcacca tcgccgatga tccccggatc accctgcatc gcggactgcc
      241 gcacgccgat ctgctccaat tgatgcgcaa ggcccacgtc ttcaccagca ccaccttcag
      301 cgaccccttc aacaacaccg tgctggaggc catgggtgcg cagttgcccg tgatctcctc
      361 cgacgtcagc tcgatcccgg agatcgtggc gcacgaacgc aacgggtggt tgctgcccgt
      421 aacggaccgg ccgagcgatg agatagccga cgacatcgcg gagcgactca agcaattgat
      481 ggaagatccg gcgctgtacc ggcggatgag cgccgaaagc ctggcggtca cccgggaaaa
      541 gttcgacctg cgtgtgcgga acgaacggct tttggcgatc tacgagcaag cgctcgcctg
      601 agcaggcact accgcctgaa acagcagtgg aagtaccgtg cctgcacgtc ccagcccgtg
      661 cccacggatg cttgcgtaca ggaggactgt ccaaccacgc gtaattgacc ggggctgttg
      721 cccgtgtcgt cggtccattc ccaatcaccc gtcaggtccg tgatgcccag ggaccccgcg
      781 ttgtagcatg ccaggtagaa ctgggcccat gaacagatcc tcatgcccag ggctccgcat
      841 acgttcacgg cttccgggaa gtcgatcgag tcgtgctggg cggtttcgat gcagtacatc
      901 gcgttcaccg ccaccgtgcc gtccggacaa ggcttccgtt cgatccggcg cgcatcgatc
      961 aattggaagg cggtgccatc gaagaccaca ctggccgtgg cacctgattg gacgtcgccc
     1021 ggtgacaggg gctggtcgcc gttcttcagc accggtatgg gcgccgaacc gttcaccgac
     1081 aaggtgacgg gtccggtgtt gccgttcgcg acgtgcaaca ggatccgaag gcctgccacg
     1141 ggtgaaggaa ccgccggttg aagcaccgcc tgccagccat cgccgctcac ctcggcgtag
     1201 agatagcccc cgccttggat cgcgcccgca ttcatggcct cgtccgccgt gaccggatcc
     1261 ttcaagcccc gcaccgcgcg atcggctgga tcggccccct cgagcacgat ggcccgatcg
     1321 ctgcgcacct gtccatgcag cattgcgacg gcacaggtgg cgaagaggag gaggagttcg
     1381 cgcatggtca tttcaacgta aggcagcacc gcacccgcgc gtagttgttg ttgctctcga
     1441 tcatggccag gttcgaaatg gaatggcaca cccatcggcc ggcgatatcg gccatgtgcg
     1501 tgtggtcact ggtgtcgtcg atccattccc aatcgttgaa caggccctcc aactcggcct
     1561 ggtgcatggt gcaggcgtgg atatactcgc catacgagca caggcgggcc ccacggtcgt
     1621 tgcaccagcg cgtggccgtg aagaaggaga cgttcaccgt gtcgttccgc tggatgcaat
     1681 actgcccgtt aacgggcata aatccatcgg gacaggtggc tggcgcgcgc gcagctgtga
     1741 agaaggccgt gtcggcgtac accaatgtgg ccaccgtgcc cggcaccagc aacccggcgc
     1801 tcaccggcag tccgtcggag cggtaaatgc gccgcgcacc gagtccgtcc acattcacgc
     1861 ttacagggcc catgcccgga gcggtcggta cgaaacgcag ttccatgccg ttgacatacg
     1921 cttcggcagg aggcgtgagt gcgagttgga tggccatgcc catgccgctg gcctggcccc
     1981 aatggtagcg tccactccgt gcaccatcga gtgtgatgag gccatccgct tccagtgcgg
     2041 gcgccgatcc ctgtaccgtg cgttcggcgg gatcggtcgc ggtgagcaca acgggtctgt
     2101 ccacatgtat ttgcgcatat gtggcgcagg tgatcaggca gcaggggagg agggcccgtt
     2161 tcatcggtcg taacagcaac gcgatcggaa cttggtcaag ggcgtttggc ggctgcccaa
     2221 gtggcaatcg cccgcagtgg acgtgtcgct ccagcccatc aatttcgcca tgttgttgta
     2281 gttggctgcg ctgtccaccc attcgtagtt cgcgatggtg gcctggaatc cgggtatgga
     2341 caggcagccc tgcaaccatt cctgcacgct gcacagacgt gtcccggcat taccgcagcg
     2401 gatcaccgcg gagtaccaat tgatggtgtc gctgggtgcc atttcgatgc acacatcggc
     2461 gttcgctgcc gcgaagcccg cagggcagcc gggatacaat tgaccggtca actggaacac
     2521 cgcaccgtcg tggatcacat gcaccgggat gccgggccgc aggtcgccgc tgtccagggg
     2581 catgttgatg ttcttccgca gcgctaccgc cccgaggccg ttcacattga gcgtggcccc
     2641 gctgttgttc gtgctggtag gcaacaaggt gagccgcagc cccggtgtgt atgtgacaag
     2701 ggctggtgtc agggtggcgg aaagttcatc cgtgcccgac accgttgcga acgtggtggc
     2761 gttg
//
LOCUS       DAOQOC010000309         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20259_length_2760_cov_3.477634, whole genome shotgun sequence.
ACCESSION   DAOQOC010000309 DAOQOC010000000
VERSION     DAOQOC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20259_length_2760_cov_3.477634"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PL106_06005"
     CDS             <1..644
                     /locus_tag="PL106_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNA32657.1"
                     /translation="RYGQPEILGVRFTGPDATRQAFRFAPDSSAAVAYYDADGNSLRK
                     AFLKAPLKFSRVSSGFTMRRFHPVQHRWKSHLGTDYAAPYGTPILAVGDGFVEKAGYT
                     AGNGNYVKIRHNGVYETQYLHMRKILVRNGQRVSQGQTIGEVGSTGLATGPHVCFRFW
                     KHGQQVDHRKEEMPSAEPVAKADRFKFEMQRDMLLAQLDEAARHGQHTSIATF"
     gene            863..1450
                     /locus_tag="PL106_06010"
     CDS             863..1450
                     /locus_tag="PL106_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32658.1"
                     /translation="MHTELTPNESLKLIESMIGQAKKSFSRISFYFLLWGALMIAAML
                     STYLLRNTDPAWSNGIAWGVAGIVGGIVSFVYGARQSKSEIVTNPMDGIIGQVWISFV
                     ITLLIMILCGGLAHRDPGPMITLLTGIPTFLTGRILRFRPLIAGGVIFWMAGIAMHLT
                     SDPLTLTVIYCGSMVFGYIIPGIMLKRQENGLRTA"
     gene            1431..1718
                     /locus_tag="PL106_06015"
     CDS             1431..1718
                     /locus_tag="PL106_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNA32659.1"
                     /translation="MAFAPLDPVLHNQLRLAVVSLLVGVDKAEFNFILEKTGATRGNL
                     SVQLTNLKDAGYIVVTKSFRDNYPLTTCKLTAKGVKAFETYVEAIKGYLKP"
     gene            complement(1708..2397)
                     /locus_tag="PL106_06020"
     CDS             complement(1708..2397)
                     /locus_tag="PL106_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNA32660.1"
                     /translation="MGKSILIAGASGSVGGRVLRKALADRRAERVVALVRRGTGLADA
                     KLDEWVAGEKDLLEGLKDQRVDAVICCLGTTIRNVGGDKAKFIHVDQELVLGLAAWAE
                     RMGVPVFSVVSAMGADARSKVFYNRVKGAMEAGLRGSAIPRTVIFQPSILTGPRKEWR
                     LGERIGIAVMRAFAPLMVGGLSGYRPMPSDVLATALLEAVFSDESGPGIHVLRYNAIH
                     ALANARRPVKA"
BASE COUNT          544 a          957 c          758 g          501 t
ORIGIN      
        1 aacgatacgg ccaacccgag atcctgggcg tgcgcttcac cgggcccgac gctacccggc
       61 aggcattccg cttcgcgccc gacagcagtg ctgccgtggc ctattacgat gcggacggca
      121 acagtttgcg caaggccttc ctgaaggcgc cactgaagtt cagccgcgta tcctccggct
      181 tcaccatgcg acgcttccat cccgtgcaac accgctggaa atcgcacctc ggtaccgatt
      241 acgcagcacc ttatggaacg cccatactgg ccgtgggcga cggtttcgtt gagaaagcgg
      301 gctacaccgc aggcaacggg aactacgtga agatccggca caacggcgtg tacgagaccc
      361 agtacctgca catgcgcaag atcctggtga ggaacggcca gcgcgtttcg cagggccaga
      421 ccatcggcga ggtcggaagt accggtctgg ccaccggacc gcatgtctgc ttccgcttct
      481 ggaaacacgg ccagcaggtg gaccaccgca aggaggagat gcccagcgcc gaacccgtgg
      541 ccaaggccga ccggttcaag ttcgagatgc agcgcgacat gctcctggcc caactggatg
      601 aggccgcgcg gcacggtcag cataccagca tcgccacgtt ctgagtccac gtacttggac
      661 ggtgcaaggg tgcgcgggat agctcccaca ccctcccacg ctcgcatccc tgcacgttcc
      721 aaccaaccgc caggaggtgc gctcaaaggc aggcgttcag cccctcatca gaggctcgat
      781 caggcgatac cacaagtctc attcctccat catttggttt atattataaa cttatctata
      841 tttgtcccct cattcaacca ccatgcacac tgaactgaca ccgaacgaaa gcctgaagct
      901 catcgaaagc atgatcggtc aggccaagaa gagtttctcg cgcatcagct tctacttcct
      961 gttgtgggga gcgctcatga tcgcggccat gctctccacc tacctcctgc gcaacaccga
     1021 cccggcctgg tccaacggca tcgcctgggg ggtggccggt atcgttggtg gtatcgtcag
     1081 cttcgtgtac ggcgcccgcc agtccaagag cgaaatagtg accaatccga tggacggcat
     1141 catcggccag gtgtggatct ccttcgttat cacactgctc atcatgatcc tttgtggtgg
     1201 cctggcccat cgggacccgg gccccatgat cacgctgctc accggcatcc ccaccttcct
     1261 gacaggccgg atcctgcggt tccgtccgtt gatcgccggg ggcgtgatct tctggatggc
     1321 cggcatcgcc atgcacctca cctccgatcc ccttaccctg accgtgatct attgcggatc
     1381 catggtcttc ggttacatca ttccaggtat catgctcaag cgtcaggaga atggccttcg
     1441 caccgcttga ccccgtcctg cacaaccagt tgcgcctggc cgtggtcagc ctgctcgtgg
     1501 gcgtggacaa ggccgagttc aacttcatcc tggagaagac gggggccacg cgcggcaacc
     1561 tcagcgtgca actcaccaac ctgaaggacg cggggtacat cgtggtgacc aagagcttcc
     1621 gcgacaacta tcccctcacc acgtgcaaac tgaccgcgaa gggggtgaag gctttcgaga
     1681 cctacgtgga agcgatcaaa ggttacctca agccttgacc gggcgccgag cgttcgccag
     1741 ggcatggatc gcgttgtacc gaagaacgtg tatcccaggc ccactctcat cggaaaagac
     1801 ggcttccaac aaggctgtcg ccagcacatc actcggcatc ggccggtatc ccgatagacc
     1861 gcccaccatc agcggggcga atgcacgcat caccgcgata ccgatccgct cgcccaagcg
     1921 ccactccttg cgcggccctg taaggatgga cggctggaag atcacagttc tcggtatcgc
     1981 tgatccgcgc aacccggcct ccatcgcgcc cttcacccgg ttgtagaaga ccttgctgcg
     2041 cgcatcagcg cccatggcgc tcaccacgct gaagacgggc acccccatcc gttccgccca
     2101 cgcggccagg cccagcacca gctcctggtc cacatggatg aacttcgcct tgtcgccgcc
     2161 cacgttgcgg atcgtggtgc cgaggcagca gatcaccgca tccacccgtt ggtccttcaa
     2221 accctcgagc aggtccttct caccagcgac ccattcatcc agcttcgcat ccgccaggcc
     2281 cgtaccgcgc ctgaccagcg cgacaacccg ctccgcacgt cggtccgcca acgccttgcg
     2341 caacacccgg ccgcccaccg atccggaagc gccagcaata aggatgctct ttcccatggc
     2401 ccaaaggaac caccgtaccc cgaaccttga gcgctcgatc gccgttggag ccgggttctt
     2461 caggtgatca catggaccct gtgtccccga tcgtcgagta ctctccggcg tcggacgacc
     2521 acagcggggg ttcgcgtcga gttcgctgaa cggcgggccc gcggatcccc gtacaccggc
     2581 ctgcggaacg ttggacatgc acaggcacct ccccccgata tccatttcgg ccatcgcggc
     2641 ggcattgctg ctgtgcccgc tcttcctgac ttcctgctcc aaggaggtgg aagtggtgcc
     2701 ggacaaccag ccgcccgacc atcacgggaa tgccctgcgg aacccgccat gtgaacggtc
//
LOCUS       DAOQOC010000310         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20271_length_2759_cov_3.422337, whole genome shotgun sequence.
ACCESSION   DAOQOC010000310 DAOQOC010000000
VERSION     DAOQOC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20271_length_2759_cov_3.422337"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PL106_06025"
     CDS             <1..563
                     /locus_tag="PL106_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32661.1"
                     /translation="ARPMRLRDSVVVVHGGKSLTSDVIAKLSSTQQNVVVATSEDIEF
                     VTGLVRELVHQAKEKRIVLVGLNAWMGMETLEARELEVLKTCVPASSWVDHSDPAVRR
                     FTLAYRERWNNEPQEWAFLGFDATYFYVTALHRFGAAFPGHFDEVGTHPLHLSFRLRK
                     TGEESGWRNETGTILRYEETGLNKVQ"
     gene            complement(683..1681)
                     /locus_tag="PL106_06030"
     CDS             complement(683..1681)
                     /locus_tag="PL106_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNA32662.1"
                     /translation="MLIASTGIASAQPPPGYYDAAYMLSGPALRSALHDIVSPHTVLP
                     YSGLWDDFVSTDERDDDPAEVWDIYSDEPGGTPGYLYNFGSDQCGSSPGSEGVCFNRE
                     HTLPVSWFNDQSPMRTDLFHIYPTDAYVNQRRGNLPYGEVGSADYTSANGTRTGSSIT
                     AGYSGEVCEPIDAFKGDLARGYFYMMTRYMDVAQQWNSDMLSNGDLSPWAEALLLQWH
                     ADDPVSPKEIERNNDVQALQHNRNPFIDNPQWAWYIWGPTAGLADIGNDGPDCWYAEG
                     SLHRRPSHEILRVVVTDAIGRTVMQGSFVGTSLLLPELPNGVYIVRGDVRTLRFAR"
     gene            1970..2467
                     /locus_tag="PL106_06035"
     CDS             1970..2467
                     /locus_tag="PL106_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32663.1"
                     /translation="MKTIVRTAALSAALLVTGLAAAQETAPVAPATNPSQAAQPVPAT
                     RSRVLDGWRSIPLGMLIKQLALTPEQTQKGKDLNSRYLKAYQALAADMPIGERKAQVA
                     QLMSTRENELKAMFTAEQLEKYKNLRTPSGELRAPVQKGTISKEPARMEPMPTAPSPA
                     EKKTE"
     gene            complement(2552..>2759)
                     /locus_tag="PL106_06040"
     CDS             complement(2552..>2759)
                     /locus_tag="PL106_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32664.1"
                     /translation="YLFKEDFEKFLVGLDDAIEEIDRLRSSGEYVHTPLPRPDATTNG
                     PGEDPPADGSFSDSGSDSRDLQDH"
BASE COUNT          541 a          841 c          872 g          505 t
ORIGIN      
        1 aggccagacc gatgcgcctg cgcgactcgg tcgtggtggt gcatggtggc aaaagcctca
       61 cgtccgatgt catcgcaaag ctcagttcca cccaacagaa tgtggtggtg gccaccagcg
      121 aggacatcga attcgttacc gggttggtac gcgagttggt ccaccaggcc aaggagaaac
      181 gcatcgtgct cgtcggcctg aacgcgtgga tgggcatgga aacactggag gcccgggagt
      241 tggaagtgtt gaagacctgt gtgccggcga gttcctgggt ggaccatagc gatcctgccg
      301 tacggcgatt cacgttggca taccgggagc gctggaacaa cgaaccgcag gagtgggctt
      361 ttctcggttt cgatgccacc tatttctacg tcaccgcgct tcatcgcttc ggtgcggcat
      421 tccccgggca tttcgacgaa gtgggaacac acccgctgca tctgtcgttc cgcttgcgga
      481 agaccggcga ggagagtggc tggcgcaatg agacgggtac gatcctgcgt tatgaggaga
      541 ccgggctcaa caaggtccag tagatcgttt tgatcgatcc cccgggtgtc acggcgggct
      601 cgtcgaaccg gtgcgatggg gctcccagtg gtacctctgt ggcactacct cgcagaggtt
      661 catggagcat gacgtggcag gatcagcgtg cgaaacgcag ggtgcgaacg tcaccacgca
      721 cgatgtagac accattcgga agctcgggca gcaggaggga ggtgccgaca aatgatccct
      781 gcatcaccgt acgcccgatg gcatccgtga ccactacccg cagtatctcg tgcgatgggc
      841 gccgatgcaa cgatccttcg gcgtaccagc agtccgggcc gtcgttcccg atatccgcga
      901 ggccggccgt ggggccccag atgtaccaag cccattgcgg gttgtcgatg aacgggttgc
      961 ggttgtgctg gagcgcctgg acatcgttgt tgcgctcgat ctccttgggg ctgaccggat
     1021 cgtcggcgtg ccattggagc agcaaggcct cggcccatgg gctcaggtcc ccgttggaga
     1081 gcatgtcgct gttccactgc tgcgccacat ccatgtaccg cgtcatcata tagaaatagc
     1141 cgcgcgccaa gtcccctttg aacgcatcga tcggttcgca cacctcaccg gagtatccgg
     1201 cggtaatgct cgatcccgtc cgggtcccgt tggcgctggt gtagtccgct gagcccacct
     1261 cgccgtaggg caggttcccg cgccgctgat tcacgtacgc atcggtggga tagatgtgga
     1321 aaaggtcggt gcgcatgggc gactgatcgt tgaaccaact caccggcaag gtgtgctcgc
     1381 gattgaagca tacaccctcg gagcccggcg atgatccaca ttggtcgcta ccgaagttgt
     1441 agaggtaccc gggtgttccc cctggctcat cgctgtagat gtcccaaact tccgcaggat
     1501 catcatcccg ctcgtcggta ctgacgaaat catcccacag cccgctgtac gggaggaccg
     1561 tgtgcgggct gacgatgtcg tgcagagcgg atcgcaacgc cggtccgctg agcatgtacg
     1621 ccgcatcgta ataacctggt ggtggttggg cggatgcgat cccggtggat gcgatcagca
     1681 tcacgaaaag caaggtaggc aaccactgga cccgccagcg gccaccggtc gcgtatgggc
     1741 gggatcccat gcgcaacgct gtggacggga gtccgtcatg gccgaccaac ctccacccgc
     1801 cggaacgcgt caaaggagtg atagcgggtt gtggggtctg ttcggcggga aacctgttca
     1861 tggcacaatg catggcgggg cgaagttcgc acaggaccct gctccgatcc gcatgggatc
     1921 ccgttcagga tcaccacctt cgcaccgcaa aaccgaacac gaccggaaca tgaagaccat
     1981 cgttcgcacc gccgcgctca gcgccgcact gcttgttacg ggcttggccg cggcccagga
     2041 gaccgcgcct gtagccccgg ccacaaaccc gagccaggcc gcgcagcccg tgcccgccac
     2101 acgctcgcgc gtgctggatg gctggcggag cattccgctg ggcatgctga tcaaacaact
     2161 cgcgcttacg cctgagcaga cccagaaggg caaggacctc aattcgcgct atctgaaagc
     2221 gtatcaggcc ctggcggcgg acatgccgat cggggagcgt aaagcgcaag tggcccagct
     2281 catgtccaca cgcgagaacg agttgaaggc catgttcacc gcggagcaac tggagaagta
     2341 caagaacttg cgtaccccga gcggtgagtt gcgcgcacct gttcagaagg gtaccatcag
     2401 caaggaacct gcgcggatgg agcccatgcc gacagcaccc tcacccgcgg aaaagaagac
     2461 cgagtgatcg gtccgcttgc ggccaagaac gtccgaggtc ccgatccgtg acggtacccc
     2521 ggtcgttccc ggggccaccg tgatgggtgt ttcagtggtc ctgcaggtcc cgactgtccg
     2581 atccgctatc gctgaagctg ccatcggcag gcgggtcctc gcccggccca ttcgtggtgg
     2641 catcgggccg cggtagtggt gtatgcacat attcacccga ggaacgcagg cggtcgatct
     2701 cctcgatcgc gtcgtccagc ccgaccagga acttctcaaa atcctccttg aacagatag
//
LOCUS       DAOQOC010000311         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20349_length_2753_cov_3.885471, whole genome shotgun sequence.
ACCESSION   DAOQOC010000311 DAOQOC010000000
VERSION     DAOQOC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20349_length_2753_cov_3.885471"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PL106_06045"
     CDS             complement(<1..878)
                     /locus_tag="PL106_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNA32665.1"
                     /translation="MIGKIRERGNLLVILVGGALLLFVLDALLSNSSGRGPGSEAVGE
                     IAGEPVSAVDFSTRVDEQAALYRDNGTNVDNQMQEQIRNSVWQEILRERTLLAQATKA
                     GLGTTLSNEEYDDIRWGDNVVSDFRNNQNFMKDGQLDRSLLKRYFAQVQTNAPGLYDY
                     QKRTFIPNRIIGKYNALIKKSCFANSAQVKEDFALNNTKATFNFVARRYDTEPDSLYA
                     VADDDVRAYFNAHRSEKKWQQKAARSFVYVSFPAAATPEDVAAVRDELAALRPEFESL
                     KGKADSLFVLHNSSSK"
     gene            complement(1235..2644)
                     /locus_tag="PL106_06050"
     CDS             complement(1235..2644)
                     /locus_tag="PL106_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNA32666.1"
                     /translation="MLFGTFRTTIGRQDQQQHLVPGAGSDPVVGAAATPSVVNDDLRP
                     VTSTQLLVLIVSMVFTALCAGLEIAIVSCNKLYVELKRKEGAIWARLVAPLLKRPGRV
                     ISALLVGNNTGLVVYGLVMADVLTPMLHEMHTSDGVVLVLETVIETLIILVVSEFIPK
                     ALFRLDPNFTLSIFALPLRLLYVILWPPTIILTLLSDGLLSLFGAKLKHGEVAFGRID
                     LDAFLKEVTDSAPADTDLDSEGEYFRNTLELSNIKVRDLMVPRAEVEAIDVEDAVADL
                     HRRFTETGLSKLLVYKDSVDNMVGYVHSYELFRKPRSIRAVMRPVHFVPGTMSADEAL
                     QRFTRQRTHVAVVVDEFGGTAGILTIEDVVETIVGDIEDEHDEGELVEERLGEREFVL
                     SARVETERLREHFGLNFPISEDYDTLAGWILHLTGEVPEQGTVIEEGPFKVTVAQVEH
                     GRIDLVKLEVVEKEEGFVE"
BASE COUNT          523 a          872 c          783 g          575 t
ORIGIN      
        1 ttcttggacg aggaattgtg cagcacgaag aggctgtcgg ccttgccttt cagcgactcg
       61 aactcggggc ggagggcggc cagttcatcg cgtaccgcgg cgacgtcctc cggggtggcc
      121 gcggccggga agctcacgta cacgaagctg cgcgcggctt tctgctgcca cttcttctcg
      181 gatcggtgcg cattgaaata cgcgcgcaca tcatcgtccg ccacggcgta caggctatcc
      241 ggctccgtgt cgtaacgccg cgcaacgaag ttgaaggtgg ccttggtgtt gttcagggcg
      301 aagtcctcct tcacctgggc actgttcgca aagcagctct tcttgatcag ggcgttgtat
      361 ttgccgatga tccggttcgg gatgaaggtg cgcttctggt agtcgtacag ccctggtgca
      421 ttggtctgca cctgagcgaa gtagcgcttc aacaggctgc ggtccaactg gccgtccttc
      481 atgaaattct ggttgttgcg gaagtcgctg accacgttgt cgccccagcg gatgtcgtcg
      541 tactcctcgt tgctcagggt ggtgccgagg ccggccttgg tggcctgggc cagcagcgtg
      601 cgctcgcgaa ggatctcctg ccagacactg ttgcggatct gctcctgcat ctggttgtca
      661 acattcgtac cgttatcgcg gtacagcgcg gcctgctcat ccacgcgagt gctgaaatcc
      721 accgcgctca cgggctcgcc ggcgatctcg cccaccgctt cgctgcccgg cccccggccc
      781 gaactgttgg agagcagggc gtccagaacg aagaggagaa gggccccgcc caccaggatc
      841 accaacaggt taccgcgctc gcggatcttg ccgatcacag ccatcgagag gatatttttt
      901 ttgaagggcc gcaaatatag gtggaggggc gttgcgggaa ggggcggacg tggcgtgggt
      961 aatgcgtggc tggttgggca gggatcacac ggccatttgc ttggctggcc aaacaagcat
     1021 tcgcctgatt tctgacttca atgatgctta tacgacgcat tatgcaaccc gcaaatgaac
     1081 gattcagcaa tcaactatct aattatcaat catatgatct tgattgtttg gccagccaag
     1141 caaattgagg cagatcccac ctgggcgccc gcgcggacga gctgaggaac ggtttggcca
     1201 gccaagcaaa ttgaggctgc tcccacctgc tcccctactc cacaaacccc tcttccttct
     1261 ccaccacctc caacttcacc aggtcgatcc gcccgtgctc cacctgggcc acggtcacct
     1321 tgaagggccc ctcctcgatc actgtccctt gttcgggcac ttcacccgta aggtgcagga
     1381 tccagccggc gagggtgtcg tagtcctcgc tgatcggaaa gttcaatccg aaatgttcac
     1441 gcaaccgctc cgtctccacc cgtgcgctca gcacgaattc gcgctccccc aagcgttcct
     1501 ccaccaattc cccctcgtcg tgctcgtcct cgatgtcgcc aacgatcgtt tccaccacat
     1561 cctcaatggt gaggatgccc gcggtgccac cgaactcgtc caccaccacc gctacatgtg
     1621 tgcgctgtct ggtgaagcgt tgcaaggcct catcagcgct catggtaccc ggaacgaaat
     1681 gcacggggcg catcaccgcc cgtatacttc gtggtttccg gaataattca tagctgtgta
     1741 catatccgac catgttgtcc acgctgtcct tgtacaccaa caatttgctc aaccccgtct
     1801 cggtgaatcg ccggtgcagg tccgcaacag cgtcctccac atctatcgcc tccacctcgg
     1861 cgcgcggtac catcaggtcg cgcaccttga tgttgctcaa ctccagcgtg ttccggaagt
     1921 actccccctc cgagtccagg tcggtgtccg caggggcgct gtcggtcacc tccttcaaga
     1981 acgcatcgag gtcgatccgg ccgaaagcca cttccccatg cttgagcttc gcgccgaaga
     2041 ggctgagcag tccatcgctc aacaaggtga ggatgatggt gggaggccac aggatgacgt
     2101 agagcagccg gagtggcaat gcgaatatgc tcaacgtgaa attggggtcc aggcggaaca
     2161 gtgccttcgg gatgaattca ctcaccacga ggatgatcag cgtttcgatc accgtctcca
     2221 gcaccagcac gaccccatcg ctggtgtgca tctcgtggag catgggcgtc agcacatcgg
     2281 ccatcaccag gccgtacacc accaggcccg tgttgttgcc cacgagcagc gcgctgatca
     2341 cccggccggg gcgcttgagc aatggggcca ccaaccgtgc ccagatggcg ccctccttgc
     2401 gcttcaactc cacatagagc ttgttgcagc tcacgatggc gatctccaac cctgcgcaca
     2461 acgccgtgaa gaccatgctc acgatgagca ccaggagttg cgtactggtc acggggcgaa
     2521 gatcgtcgtt gacaacggaa ggtgtggcgg ccgcccccac cacaggatcg gatcctgctc
     2581 ccgggaccag atgctgctgc tggtcctgtc gtccgatggt tgttcggaag gtgccgaaca
     2641 gcatggtaac aggtcctcag cccggttcac cttgcatctg gacgttttat tgtacccgat
     2701 cgatcttccg aacggctccg gatggtccgc cgaagagggg cgtgaccgcg agc
//
LOCUS       DAOQOC010000312         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20395_length_2750_cov_3.551763, whole genome shotgun sequence.
ACCESSION   DAOQOC010000312 DAOQOC010000000
VERSION     DAOQOC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20395_length_2750_cov_3.551763"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2243)
                     /locus_tag="PL106_06055"
     CDS             complement(<1..2243)
                     /locus_tag="PL106_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32667.1"
                     /translation="MASTLRVPRIPVLRTLSVLRNVLLAVCATLLWGDEAHATHAVGG
                     QLTYQCVGVNQYLVTLKFYRDCNGVAAPTNCTNGRRFNVKSASCGADFNQCFSLQSVN
                     IITPICPSEIDRCLDNSGTYGLEEYIFTKLVDLSAYAGCGNPNDWVFSWSLCCRNNAI
                     TSLSDPGDEDLYLRTEMNRQAVACNNSPTFGNSPAPYYCLGQSVSYNPGAFDVDGDSL
                     AYEIIQPRGTNNSTIPYSSGYSLPQPIHNTGGAGAVQLDPLTGTLTCVPDIQQVAVVT
                     YRVREYRNGVLIGTVMRDVQIVVRPCGANTAPSASGINGTNNYSTTVCAGSPVTFTIN
                     TSDPNAGQGTVITWNGGIAGATFTTSGSPFQTATFSWTPTLADIGNNNFAVQVTDNAC
                     PLSGSNQYGFTLVVTPPLTPANAGTDLVSCGSATMQAQQPYPQIPGTWSVITGSGTFT
                     PPDAPNAQVTGMAVGTNVYQWSMNYGLCGISTDQVTVTNYDPGQAAANAGPDQALCMP
                     FNTAVLAANTAVAPAVGTWTVVQGAGTFSNANSPTSTVSGLVLGINRFRWTINNGPCA
                     APTQDEVVITVNQPVAAANAGPDQSVCAPGAGAQLNAAPAPSPATGLWTVLGGSSAVV
                     TTPGSPTSTVTGLTPGTHSFQWTVSNAGCGSTSDQVSFTVYDPASPNANAGPDQTICA
                     ANTTLAGNAPISPATGQWTLVSGTGTITTPGSPTSGVTGLGVGANVFQWTLNNGPCAN
                     GLTTDQVT"
     gene            complement(2605..>2750)
                     /locus_tag="PL106_06060"
     CDS             complement(2605..>2750)
                     /locus_tag="PL106_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNA32668.1"
                     /translation="YVALSYDHRIIDGKESVSFLMAVKQMIEDPDKMVFGNREPGEVL
                     LGL"
BASE COUNT          487 a          860 c          878 g          525 t
ORIGIN      
        1 gttacctgat cggtggtaag accgttcgcg cacggaccgt tgttgagcgt ccactggaac
       61 acattcgccc cgacacccag tcccgtgacg cccgaggtgg gcgatcccgg ggtggtaatg
      121 gtgccggtcc cgctcaccag cgtccactgt ccggtcgccg gcgagatggg cgcgttgccg
      181 gcgagcgttg tattggccgc acaaatggtc tgatccggtc ccgcattcgc gttgggtgat
      241 gctggatcat acaccgtgaa gctcacctgg tcgctggtgg aaccgcagcc cgcgttgctc
      301 acggtccact ggaagctgtg cgtgcccggc gtgagaccgg taaccgtgga cgtcggcgaa
      361 cccggggtgg tgaccaccgc acttgacccg cccagcacgg tccacaggcc agtggctggc
      421 gatggggccg gagcagcgtt cagttgcgca cccgcaccag gagcgcacac actttggtcg
      481 ggcccggcgt tggctgcggc caccggttga ttcaccgtga tcacaacctc atcctgggtg
      541 ggcgcggcac acggcccgtt gttgatcgtc cacctgaagc ggttgatccc gagaacgagg
      601 cccgaaaccg tggacgtggg cgaattcgca ttgctgaatg ttccggctcc ttgcaccacc
      661 gtccatgtgc ccaccgcagg ggccaccgcc gtgttggccg cgagaaccgc cgtgttgaaa
      721 ggcatgcaaa gcgcctgatc agggccggca ttcgccgcgg cctggccggg gtcgtagttc
      781 gtcaccgtga cctgatcggt cgagatacca caaagcccgt agttcatgga ccactggtac
      841 acgttggtcc caacggccat gcccgtcact tgcgcattgg gagcatcggg cggcgtgaat
      901 gtgccgctgc cggtgatcac actccatgtc cccggtatct gcggatatgg ctgctgcgcc
      961 tgcatcgtcg cactgccgca actgacaagg tcagtgcccg cgttcgccgg ggtgagtggt
     1021 ggggtcacca cgagtgtgaa accatactgg ttgctgcctg aaagcgggca tgcgttgtcg
     1081 gtcacttgga ccgcgaaatt gttgttaccg atgtcggcga gcgtaggcgt ccaactgaag
     1141 gtcgccgtct ggaagggcga gccgctggtg gtgaacgtgg cgcccgcgat accgccgttc
     1201 caggtgatca ccgtgccttg tccggcattc ggatcgctcg tattgatggt gaaggtcacc
     1261 ggcgagcccg cgcacaccgt ggtggagtag ttgttcgtac cgttgatgcc gctggccgag
     1321 ggtgcggtgt tggccccgca cggccgcacc acgatctgca catcgcgcat caccgtgccg
     1381 atgagcacgc cgttgcggta ctcgcgcact cgatacgtga ccaccgccac ctgctggatg
     1441 tccggcacgc acgtgagagt gccggtcaac gggtccaatt ggaccgcacc cgcacctccg
     1501 gtgttgtgga tcggctgcgg caggctgtat ccgctgctgt acgggatcgt gctgttgttg
     1561 gtaccacgcg gttgaatgat ctcgtacgcc agtgaatcgc catccacatc aaaggctccc
     1621 gggttgtagc tcacggattg gcccaggcag tagtacgggg ccgggctgtt gccgaacgtc
     1681 ggcgagttgt tgcaggccac ggcctgccgg ttcatttcgg tgcgcagata caggtcctca
     1741 tcccccgggt cgctcaggga ggtgatcgcg ttgttgcggc agcacaacga ccaactgaac
     1801 acccaatcgt tcgggttgcc gcaaccggcg taggcgctga gatccaccag cttcgtgaag
     1861 atgtactcct ccaggccata cgtcccgctg ttgtccaggc atcgatcgat ctccgagggg
     1921 cagatcggcg tgatgatgtt cacgctctgc agactgaagc actgattgaa atcggccccg
     1981 caactcgcac tcttgacgtt gaaacgccga ccgttggtgc agttcgtcgg tgctgcgacc
     2041 ccgttgcaat cccgatagaa cttcaacgtc accaggtact ggttcacacc gacacactgg
     2101 tatgtgagct gtcctcctac agcatgcgtg gcatgcgctt catcgcccca caagagcgtg
     2161 gcacacacgg cgagcaggac gttgcgaagg acggacaggg tccggaggac cgggatgcgt
     2221 gggacgcgta gcgttgatgc catgaaccag cgggatgtcg gcgttcggcc acgtagtacg
     2281 gcagacggcc gtgcggagcg catggcccac cgtacaggtg gggaagggtg ccggacaaac
     2341 gtacccgggg ggctccggac accccctccg gaaagacctc acttgtcaac atggcgatgt
     2401 tcggtggcag agtgtttgtt ctcccaaaaa caggtatcga tccgaagtga tcgtgcgcat
     2461 cgggacacgg atcacctccg tatgcgccgt gcgttcgcga cccggaaatg ggacggcagg
     2521 gccagtatac ccctcgcttt cagcgcgccg accgggcgac cggaccaacc tccggcacgg
     2581 atcgatccac cgatgctgaa ccggtcacag gccgagcaac acctcgcccg gctcacggtt
     2641 cccgaacacc atcttgtccg ggtcctcgat catctgcttc acggccatca ggaaactcac
     2701 gctctccttg ccgtcgatga tgcgatggtc gtagctcagc gccacgtaca
//
LOCUS       DAOQOC010000313         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20456_length_2745_cov_2.891078, whole genome shotgun sequence.
ACCESSION   DAOQOC010000313 DAOQOC010000000
VERSION     DAOQOC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20456_length_2745_cov_2.891078"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="PL106_06065"
     CDS             complement(<1..1567)
                     /locus_tag="PL106_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HNA32669.1"
                     /translation="MKQILVASASFWTFSLAAQTIPLWLRNAAISPDGQTIAFCYQGD
                     IWRVPTAGGDAIPLTTSEAYDHTPVWSHDGARIAFTSNRYGNSDVFVMASMGGAPVRL
                     TFHSNGETASDFTRDNSQVIFYGTRQDDVRDQQFPVGGLGELYTVPSNGGRPLQISTI
                     AMQNARYNPAGTIIAYHDRKGYEDNWRKHHTSSVTRDIWTFNPATKAYTQLTTFAGED
                     RDPVWSKDGNTLYYLTEEKGSFNIHKMAAAGGASTQVSHFTGDPVRYLSMSDAGVLCF
                     SHRGELYTMTDGGEPRKVDIRIHTDGRYLPERTINVNSADEYSVSPNGKEIVLVHRGE
                     VFVTSVAEGTTRRITNTPEQERSVSFSPDGRSILYASERNGSWDLYTTSLTRNEEKYF
                     FNGTVLKEEPLLNTPAEEFQPYYSPDGKEVAYLEERTTLKVLNLATKQTRTIMPGNRN
                     YSYSDGDQWYQWSPDSKWFTVGFLHDKQWINQVGLVSAEGGKEITDLTRSGYGGYTPR
                     WAMDGKAIVYVSDR"
     gene            1689..2186
                     /locus_tag="PL106_06070"
     CDS             1689..2186
                     /locus_tag="PL106_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32670.1"
                     /translation="MEPTTSRRTIEDMPAHVRVWVYKSATPFTPDQQREMLLRGMAFS
                     GEWTAHAVALDACVDVLYGHFLVIAVDERQALASGCSVDKSVRFIQELERDLMLTLTD
                     RMVVLYDGDPGIRACRVPEVEGLIRSGGMHADTLVYDDLVTTCGELRARFRGPLRGTW
                     LARYL"
     gene            2286..2693
                     /locus_tag="PL106_06075"
     CDS             2286..2693
                     /locus_tag="PL106_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32671.1"
                     /translation="MRALYERLFGSFSQGWGQLTVYFLLLLLGSLILSWLSNRKLRPV
                     DRGPALSVPMFLASFAMLMIARYFILDRWNAVIIAAGLAIAGLFISKSGTRAPVLIVI
                     MLAVLLGFGLNLSAMVLFVAAFIMLLLSRTAVK"
BASE COUNT          439 a          800 c          894 g          612 t
ORIGIN      
        1 cgcgatcact gacgtacacg atcgccttgc catccatggc ccagcgcggc gtataaccgc
       61 catagccgct ccgggtgagg tccgtgatct ccttgccgcc ctcggcactc accaggccca
      121 cctgattgat ccactgcttg tcgtgcagga aacccaccgt gaaccatttg ctgtccgggc
      181 tccattggta ccactggtca ccgtcgctgt agctgtagtt cctgttcccg ggcatgatgg
      241 tgcgggtctg cttggtggcg aggttcagga ccttgagcgt ggtgcgttct tccaggtagg
      301 ccacttcctt gccgtccggt gagtagtagg gctggaattc ttcggccggt gtgttgagca
      361 ggggttcctc cttcagcacg gtgccattga aaaaatactt ctcctcgttg cgggtgaggc
      421 tggtggtgta caggtcccag ctgccgttgc gttcgctggc gtaaaggatg ctgcgcccat
      481 ccgggctgaa actgacgctg cgctcctgtt cgggcgtgtt ggtgatccgg cgcgtggtgc
      541 cctcggccac ggaggtcacg aagacctcgc cgcggtgaac aaggacgatc tccttgccgt
      601 tggggctcac gctgtactcg tccgcgctgt tcacattgat cgtgcgttcg ggcaggtaac
      661 ggccatcggt atggatccgg atgtccacct tcctgggttc gccgccatcg gtcatggtgt
      721 acagctcgcc cctgtggctg aagcagagca ctcccgcatc gctcatgctg aggtagcgca
      781 cgggatcccc ggtgaagtgg ctcacctggg tgctggcacc acccgcggcc gccatcttgt
      841 ggatgttgaa gcttcccttc tcctcggtga ggtagtacag ggtgttgccg tccttgctcc
      901 acaccggatc gcggtcctcg ccggcgaacg tggtgagttg cgtgtacgcc ttggtcgcag
      961 ggttgaacgt ccagatgtcg cgcgttaccg aggaggtgtg gtgcttgcgc caattgtcct
     1021 cgtagccctt gcgatcatgg taggcgatga tcgttcccgc cgggttgtac cgggcgttct
     1081 gcatggcgat ggtacttatt tgcagcggcc gtccgccgtt cgatggcaca gtgtacagct
     1141 cgcccagccc gcccacaggg aactgctgat cgcgcacatc gtcctgccgg gtaccgtaaa
     1201 agatcacctg actgttatcg cgggtgaagt ccgaagcggt ctcgccgttg ctatggaatg
     1261 tgaggcgtac cggcgcaccg cccatgctgg ccattacgaa cacatcgctg ttaccgtagc
     1321 ggttgctggt gaatgcgatc cgcgcaccgt catggctcca gaccggggta tggtcgtagg
     1381 cctcgcttgt ggtcaggggg atggcgtcac cgccggcggt gggcacccgc cagatatcgc
     1441 cctggtagca gaacgcgatg gtttgcccat cggggctgat cgcggcgttg cgcagccaga
     1501 gcggaatggt ctgggcggca agggaaaacg tccagaagga agcggaggcg acaagtatct
     1561 gtttcattgt tgttggggcc gggtcgcatt cggcccgacc cttcgtggca tcgcgaccgg
     1621 ccgccaaact acccgccatg ccgccccgat ggtcacggga cgaacggttc tgtgccaacc
     1681 tttgcaaaat ggaaccaacg accagtcgcc gaacgatcga ggacatgccg gcccatgtgc
     1741 gggtgtgggt gtacaagagc gcaaccccct tcacaccgga tcaacagcgg gagatgctct
     1801 tacgaggaat ggcgttctcc ggggaatgga ccgcgcatgc cgtggcactc gacgcctgtg
     1861 tcgatgtgct gtacggccat tttctggtga tcgctgttga tgaacgacag gccctggcca
     1921 gcggatgcag cgtcgataaa agcgtgcgtt tcatccaaga actggagcgt gatctgatgc
     1981 tgacgctaac cgaccggatg gtggtcctgt acgatgggga tccgggtatc cgggcgtgcc
     2041 gggtgcccga ggtggaaggt ctgatcaggt cgggcggaat gcatgccgat acattggtct
     2101 atgacgacct ggtgaccacg tgcggcgaac tgcgtgcgcg gttccgtggt cccttgagag
     2161 gtacctggct ggcgcgttac ctgtgatggt acagggttcg tgatggtcgc tatgcacggc
     2221 gacctgttgg cgaaggaacc catgggcctg ccggacaggg ttgtccgact gcgcaccttg
     2281 cgccgatgcg tgcgttgtac gaacgtttgt tcgggtcctt ttcccaaggg tgggggcagt
     2341 taacggtcta tttcctgctg ctgctgctcg gttcgttgat cctgtcctgg ctgtccaacc
     2401 ggaagctgcg accggtcgat cgtggtccgg cgttgagcgt gcccatgttc ctggcatcat
     2461 tcgccatgtt gatgatcgcc cggtatttca tactggaccg atggaacgcc gtcatcatcg
     2521 ccgccggcct ggccatcgcg ggcctgttca tctcgaagag cggcacgcgc gcaccggtgc
     2581 tgatcgtgat catgcttgcc gttctgctcg gcttcgggtt gaacctgagc gccatggtgc
     2641 tgttcgttgc ggccttcatc atgttgctgc tcagccgcac cgctgtgaaa tgaggtcgaa
     2701 gcgcaagaag ggatccacac cggtggccct gctgaccggc ctggg
//
LOCUS       DAOQOC010000314         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20488_length_2743_cov_4.360863, whole genome shotgun sequence.
ACCESSION   DAOQOC010000314 DAOQOC010000000
VERSION     DAOQOC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20488_length_2743_cov_4.360863"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PL106_06080"
     CDS             <1..258
                     /locus_tag="PL106_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32672.1"
                     /translation="VDGRVAFLASDGGFLCAELDQHQQLAATAADLGDYGLFTVVPLD
                     NDQVALRAFNGRYLELDSVSMEMSAKANDANRWTRFRVVKP"
     gene            complement(298..1749)
                     /locus_tag="PL106_06085"
     CDS             complement(298..1749)
                     /locus_tag="PL106_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32673.1"
                     /translation="MGLGLAALLLVGCGGQVPSGFVRLENGRFHVDGKPFFPIAVNYV
                     MHLQFNDSACWAAPFRNYEREDRFRYQTKDSTHRLIKAEFELIRKLGFNTVRITSLAS
                     DMKLQGDSLYPWLPAQYGWGKDSLYPLNGDRVEHYFDAVDDMVLLAEETGLKLIILVK
                     LFPGEPSYLRQALKLVDRLRDRPGVLAYDFFNEPLYFDVHDRPKREVHELVKQWQKRF
                     KAHGPNQLTTIGLVGVPEVFAWDPNVLAVDFVSFHPYEYEPNQVMSEIHWYGKHVDKP
                     WIVGETSLPADNDSVPYAEQLSFAHRTLAQTVACGGQGYSWWQFKDVKWGRFHSDYMG
                     VLSMAGETQVAPDLPMVHGTVKPVWQAFSTFDPTAPPGPCIVPDNYYNFSSLTGAKLT
                     GKLLDRDRRPIEGGVIHGWNEHWTRSYYTKSKADGSFELFGDFRFHHWMASATRCSMV
                     RGDCDPNAFYTGADSIPAYYLGELTLEDLHLGE"
     gene            complement(1783..2511)
                     /locus_tag="PL106_06090"
     CDS             complement(1783..2511)
                     /locus_tag="PL106_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA32674.1"
                     /translation="MVGPAPLRALSIIIPAYNEERTIHLILDKVRNVQLINGIAKEVI
                     IVNDGSTDGTVAAVERYMGINPAMGIRFIQQPRNMGKGAAIHRGIKEATGEYLIVQDA
                     DLEYDPREYNDLLRPVIEGFADVVFGSRFMGHHPHRILFFWHSIGNKFLTQLSNAFNN
                     LNLTDMETCYKLIRSDIAKSLDLCEKRFGFEPEVTAKLAKVKEARIYEVGISYYGRTY
                     AEGKKIGWKDGFRAIWCIVKYGVG"
     gene            complement(2544..>2743)
                     /locus_tag="PL106_06095"
     CDS             complement(2544..>2743)
                     /locus_tag="PL106_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32675.1"
                     /translation="VQRLIDLGASYLFVVNERWLAEPYLQRFYRYPLGQHRSVRVFDL
                     RPFQGPDHRVPHAWGHAPPTM"
BASE COUNT          546 a          821 c          833 g          543 t
ORIGIN      
        1 gtggatgggc gcgtggcctt cttggcctcc gacggaggct tcctctgcgc ggaattggat
       61 cagcaccagc agctggccgc cacggccgcg gacctgggcg actacggcct cttcaccgtg
      121 gtgccgctgg acaatgatca ggtggccctc cgggcattca atgggcgcta cctggaattg
      181 gacagcgttt caatggaaat gtcggccaag gcgaacgacg cgaacagatg gacccggttc
      241 cgggtggtga aaccatgagc acagcggttg cagccatccg cgtgtttcgc aggcaggcta
      301 ttcgcccaag tgcaggtcct ccagagtcaa ctcgcccagg tagtacgcgg gtatgctgtc
      361 cgcgccggta taaaatgcat tcggatcgca atcgccgcgc accatggagc agcgcgtggc
      421 cgaagccatc caatggtgga agcggaaatc gccgaacagt tcaaagctgc catcggcctt
      481 ggacttggtg tagtacgaac gggtccaatg ctcgttccaa ccatggatca caccgccctc
      541 gatgggcctg cgatcccggt cgagcaattt gccggtgagt ttggcgccgg tgagcgagga
      601 gaagttgtag tagttgtcgg gcacgatgca gggccccgga ggcgctgtcg gatcgaatgt
      661 ggagaaggcc tgccacacgg gcttcacggt gccatgtacc atcggcaggt cgggcgccac
      721 ttgtgtttcc ccggccatgc tcagcacgcc catgtagtcc gagtggaagc ggccccattt
      781 cacatccttg aattgccacc atgaatagcc ttggccgcca caggccacgg tttgtgccag
      841 cgtcctgtgc gcgaaggaca gctgctccgc gtagggcacc gaatcattat ccgcgggcaa
      901 cgaggtttcg ccgacgatcc agggcttgtc cacgtgcttg ccataccagt ggatctcgct
      961 catcacctgg ttcggctcgt actcgtacgg gtggaaggaa acgaaatcca cggcgagcac
     1021 attggggtcc caggcgaata cctccggcac gccaacaagc ccgatggtgg tcaactggtt
     1081 cgggccatgt gccttgaacc gcttctgcca ctgcttcacc agttcatgga cctcccgctt
     1141 cggacggtcg tgaacatcga agtagagtgg ttcgttgaag aagtcgtagg ccagaacgcc
     1201 cggacggtcc cgcagccgat ccaccagctt gagcgcctgc ctcaggtagc tcggttcacc
     1261 gggaaagagt ttcaccagga tgatcagttt caagccggtc tcctccgcca gcagcaccat
     1321 gtcgtccacc gcatcgaaat agtgctcgac acgatccccg ttcagcggat acaaggaatc
     1381 cttgccccag ccgtattgtg cgggtagcca gggatagagc gaatcaccct gcagtttcat
     1441 gtccgaagcc aggctggtga tccgaacggt gttgaagccc agttttcgga tcagctcaaa
     1501 ctccgccttg atcagtcggt gggtggagtc cttcgtttgg tagcggaagc ggtcctcccg
     1561 ctcgtaattc ctgaatggcg cggcccaaca tgcgctatcg ttgaactgca ggtgcatgac
     1621 gtagttcacc gctataggga agaagggctt gccgtccacg tggaaccggc cgttctccaa
     1681 gcgcacgaag ccactcggaa cctgaccccc gcagcccacc aaaagcagtg ctgcgagccc
     1741 gagtcccaag aggtaccgga atggaatgca cccggccatg ccctaaccaa cgccgtattt
     1801 cacaatgcac cagatcgccc ggaaaccgtc cttccagccg atctttttcc cttcggcata
     1861 cgtgcggccg tagtagctga tgcccacctc gtagatccgg gcctccttca ccttggccag
     1921 cttggcggtg acctcgggct caaagccgaa gcgcttctca cacaagtcga ggcttttggc
     1981 gatgtcgctg cggatcagct tgtaacaggt ctccatgtcc gtcaggttca agttgttgaa
     2041 cgcgttggag agctgcgtga ggaacttgtt gccgatgctg tgccagaaaa acaggatccg
     2101 gtgggggtgg tgccccatga agcgcgaccc gaagaccaca tcggcgaagc cttcgatgac
     2161 cggacgcaac agatcgttgt actcgcgcgg atcgtactcg agatcggcat cctgcacgat
     2221 caggtattca cccgtggctt ccttgatccc gcgatggatc gccgcaccct tgcccatgtt
     2281 gcggggctgt tggatgaagc ggatgcccat ggcggggttg atccccatgt accgctccac
     2341 ggcggctacc gtgccatcgg tactgccgtc gttgacgatg atcacttcct tggcgatgcc
     2401 attgatcagt tgaacattgc gcaccttgtc caggatcaga tggatggtcc gttcctcgtt
     2461 gtaggcgggg atgatgatgg agagtgcgcg cagtggggca ggtccgacca tggcgcgcga
     2521 agataccggc ggcgtggtgg ctcctacatg gtgggcggcg catgacccca cgcgtgaggc
     2581 actcgatggt cgggaccctg gaacgggcgc aggtcgaaca cccgtaccga acggtgctgc
     2641 ccgaggggat accggtagaa acgctgcagg taaggttcgg ccaaccaccg ctcgttgacc
     2701 acgaacagat atgaagcacc caggtcgatc aaccgttgca cat
//
LOCUS       DAOQOC010000315         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20514_length_2741_cov_4.201415, whole genome shotgun sequence.
ACCESSION   DAOQOC010000315 DAOQOC010000000
VERSION     DAOQOC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20514_length_2741_cov_4.201415"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PL106_06100"
     CDS             complement(<1..835)
                     /locus_tag="PL106_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNA32676.1"
                     /translation="MTDLERIAQQPASALEAAADKRILVLDGAMGTMIQKLGLTEEDF
                     HPKGMEDHKILLKGNNELLSLSRPEAIRTIHEQYLEAGADIVETNTFSATSIAQAEHA
                     CSHLAREMNLASVRLAKEACAKYTAMDPSKPRFVAGAIGPMNKTASLSPDVNDPGFRA
                     VTFDQLVTAYKEQAEALLDAGADLLLVETIFDTLNAKAAFFAIDEVFEARNTRVPLMC
                     SVTITDASGRTLSGQTIEAFLISMAHVPLFSMGLNCALGAALMRPYLEVIAEQSPCRV
                     SI"
     regulatory      complement(1047..1204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1251..2135)
                     /locus_tag="PL106_06105"
     CDS             complement(1251..2135)
                     /locus_tag="PL106_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32677.1"
                     /translation="MQRVDVAINVYGKPRQTAVTLLSLLMHSGEHIGRIHFTLERRQP
                     SGASFRSLIGHLGDRVVVHRPLFWFGVRPQRSKWLMRWSPYRQSLRYQHAWETSTAPY
                     LFITHNDVLYEGDILGAMLGRVGEHVAIGPVGQCWNCPASYAQRCSPKSFMRFHPSFR
                     EWQQLARDHPGPRAAHYDRVVDPQRPWPLPECRINEWSMLVNQERARPLTMPHGNAVP
                     LGALYGLDIGTQWFHDVLNSRASVEHFDIAAYATHAWASSTGGGHAALADRGIYHTEE
                     TRAATWLKQNHPDFPLTN"
     gene            2289..>2741
                     /locus_tag="PL106_06110"
     CDS             2289..>2741
                     /locus_tag="PL106_06110"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA32678.1"
                     /translation="MRTTALTLALLTTGLVPAQSFIAQVKPAGSELWGYMSNTGEMVV
                     PPTYKHCFRFNTSGYAAVLDPSTKNGAFINTKGERLATTAPAFQLIGGMMSGGDVQGF
                     SCGLAPVKVGEKWGFLNPRGELTVPATYDKVDAFESCLSVVEKGKKLFI"
BASE COUNT          511 a          815 c          869 g          546 t
ORIGIN      
        1 agatgctgac gcggcagggg ctctgctcgg cgatcacctc gaggtacggc cgcatcagcg
       61 ccgcgcccag cgcgcagttg aggcccatgc tgaagagcgg cacgtgcgcc atgctgatca
      121 ggaaggcttc gatggtctgg ccgctgaggg tgcgcccact ggcatcggtg atggtgacgc
      181 tgcacatgag cggcacgcgc gtgttgcgcg cttcaaagac ctcgtcgatg gcgaagaagg
      241 cggccttggc gttgagcgtg tcgaagatgg tctccaccag cagcaggtcg gcgccggcat
      301 cgagcagggc ttccgcctgt tccttgtagg cggtgacgag ttgatcgaag gtgacggcgc
      361 ggaagccggg atcgttcacg tcgggactga gcgatgcggt cttgttcatg ggcccgatcg
      421 caccggccac gaagcgcggc ttgctcgggt ccatggcggt gtacttcgcg cacgcctcct
      481 tcgccaggcg caccgaggcc aggttcatct cacgtgcgag gtggctgcac gcgtgctcgg
      541 cctgcgcgat gctggtggcg ctgaaggtgt tcgtctccac gatgtcggca ccggcctcca
      601 ggtattgctc gtggatggtg cggatggcct cgggccgcga gagggagagc agttcgttgt
      661 tgcccttcag caggatcttg tggtcctcca tgcccttcgg atggaagtcc tcctcggtga
      721 ggccgagctt ctggatcatg gtgcccatgg ccccgtcgag gacgaggatg cgcttgtccg
      781 ctgcagcttc caaagcggag gcgggttgtt gggcgatgcg ttcgaggtcg gtcattgcgt
      841 gttcctatgt cggcgtgctt tccgagctat tgcggtgaga atggtgaaat gggtgaaagt
      901 ggtgagaaag tgggccacgg agcgccagca ctttcttacc attctctcca ccctcaccac
      961 cttcaccgca ctccaaaaaa gaaagcccca cccgatgttc cggatgaggc atcaatgttc
     1021 cctggcgggc actgctgtct gatcgcggct catctttccg ttgttgtcgc tcctcgtcct
     1081 tcgactgcgc tcaggagcgc tcggcgtgac aacaacagct ggatttagca cctacccgat
     1141 ggttgcccac cggacggttg ccaaggcttc gcagggccag tccctccgcc tttctggata
     1201 agcgatggaa agaacgtggg gcgaagatag cgtgacgaac atgatcgtcg tcagttcgtg
     1261 agcgggaagt cgggatggtt ctgtttcagc cacgttgctg cccgtgtttc ctccgtgtgg
     1321 tagatcccac gatcagccaa ggcggcatgt cctcctccgg tggaactggc ccaggcatgc
     1381 gtggcgtagg ctgcaatgtc aaaatgctcc accgatgccc tgctgttcag cacatcgtgg
     1441 aaccattgcg taccgatgtc caagccatac aacgcaccga gtggaacggc gttcccgtgt
     1501 ggcatggtca agggccgcgc gcgttcctgg ttcacgagca tgctccactc attgatccgg
     1561 cattcgggga ggggccaagg gcgctgcgga tcaacaacac ggtcgtagtg cgcagcacga
     1621 ggaccgggat gatccctggc cagttgctgc cactccctga acgacggatg aaagcgcatg
     1681 aaactcttcg gtgaacaacg ctgcgcgtat gaggccggac agttccagca ctggcccacc
     1741 gggcctatcg ccacatgttc gccgacccgg cccaacatcg caccgaggat atcgccttcg
     1801 tacagcacat cgttgtgcgt gatgaagagg tagggtgcgg tgctcgtttc ccaggcgtgt
     1861 tgatagcgta acgactgccg gtatggcgac cagcgcatca gccatttgct ccgttgcggg
     1921 cgcacgccga accagaacaa gggcctatgg accacgacgc gatcacccag gtgtccgatc
     1981 aaggagcgga aggaagctcc agagggctgg cggcgttcga gcgtgaagtg gatccggccg
     2041 atgtgttcgc ccgaatgcat caacaacgaa agcagggtca ccgctgtctg gcgcggcttg
     2101 ccgtacacgt tgatggcgac atccacacgt tgcatcggcc gcaatatgga gccgatcgtg
     2161 catggtgatc cgcctgaaag agacccccgc tacggacttt ggttccacaa ggctgttctt
     2221 cccgggcaag gtcaagccgg gtagcgcatc catctgttcg catctttggg gatccagttc
     2281 aacccgtcat gcgcaccaca gccctcaccc tcgctctcct gaccaccggt ctcgtcccgg
     2341 cccagtcctt catcgcccag gtgaaacccg ctggctcgga actatggggc tacatgtcaa
     2401 ataccggaga aatggtggtc ccacccacct acaagcattg cttccgtttc aacacgagcg
     2461 ggtatgcggc cgtgctggac ccctccacga agaacggcgc cttcatcaat accaagggtg
     2521 aacggctggc caccaccgct cctgccttcc aactgatcgg tggaatgatg agcggcgggg
     2581 atgtgcaggg cttcagctgt ggactggcac cggtgaaggt cggtgaaaaa tggggcttcc
     2641 tgaaccccag gggcgaattg accgtccccg ccacgtacga caaggtggac gccttcgaga
     2701 gttgcctgag cgtggtggaa aagggcaaga aactgttcat c
//
LOCUS       DAOQOC010000316         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20558_length_2739_cov_2.709389, whole genome shotgun sequence.
ACCESSION   DAOQOC010000316 DAOQOC010000000
VERSION     DAOQOC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20558_length_2739_cov_2.709389"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PL106_06115"
     CDS             <1..635
                     /locus_tag="PL106_06115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA32679.1"
                     /translation="VRDEATMDRLRRRFGLVGQGPVFGTLARFIPWKGYTHIVDAAHI
                     LVQHHPHARFLFCGTGPQEEEIRARVAELGLHDHIIFTGWVDRDDVPSLFGIMDGYLH
                     AADHEPFGFVYAEAMMAGVPVVSTPTGAALDAIEDGVSGILVKERSGKSLAEGVERLL
                     RMDGPLLGRNGRAIALRLFDLEVMYRGYKDLYLGMLQLPGTERRDPATAP"
     gene            632..1504
                     /locus_tag="PL106_06120"
     CDS             632..1504
                     /locus_tag="PL106_06120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA32680.1"
                     /translation="MSDLVSVIVPAYNAGPWIGEAVRSVLAQTHRHVEVIVVNDGSTD
                     DTLERVLEIPDPRLRVVDQANAGVSAARNMGLALAKGDLIHFMDADDAMLPENLAVKI
                     QGLKDHGTDWVYGDLLICDADLKPTGERLRGTDGDVTRTVLLGATTAVPTPCSNVLAR
                     RRCYDDGIRLDEHLSNAADQDLAIHLAQRHTYKRLNVALNLYRIVPGSMSKSIPLFER
                     DHLRLFRKADAAGLLQGTLFRRRCLANLYWAIGGSWWKFTDERARALRFFLKAVMSHP
                     AVLVRPVRKRLVFR"
     gene            1539..>2739
                     /locus_tag="PL106_06125"
     CDS             1539..>2739
                     /locus_tag="PL106_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNA32681.1"
                     /translation="MSIQRSILHTFLAQVPTLALFFLASVLMTNVLGDDGRGAFALMQ
                     NLVIFMMMGLGLNFSLGLMYHTARSGGDQRVPVGMASSLFLVNLVAVPSILGIIIFSD
                     ALRGIFFPGRLAHPIYFAYVAVSVLMGQVITFVSAMVQGLKLFTILNRMSIVTAAFSC
                     TGFVLIWCFRGMIDPANILPYTLLVTVSGVTMQAVIWSIIYVRKIGLPPVPLRDWTVM
                     RPFLAFSLMGYLTNMINLVNYRLDIWIVDQFSGTANLGLYAAAVGVGQLFFNIPEPLS
                     RVVQPYLYSSNDDGMMERFRVIARLNFTAVTVLCIFTAIIAPWLLPLMYGPDFSGSVT
                     ALRLLLPGIAFCGAYKLLAVVLIQRGLLRFNLYGSVIAALTTVVLDLVLIPAMGITGA
                     SIASSIAY"
BASE COUNT          466 a          870 c          850 g          553 t
ORIGIN      
        1 cggtacgcga tgaagccacc atggacaggc ttcgacggcg attcggcctg gtgggccagg
       61 ggcccgtgtt cggaacactc gcccgcttca tcccctggaa aggctacacg cacatcgtgg
      121 acgccgcgca catcctggtg caacaccacc cgcacgcacg gttcctgttc tgcggcaccg
      181 gcccccagga ggaggagata cgtgcccggg tcgctgaact cggcctgcac gaccacatca
      241 tcttcaccgg gtgggtcgat cgggacgatg tgccttcgct cttcggcatc atggacggtt
      301 atctgcacgc ggccgatcac gaacccttcg gtttcgttta cgccgaggcg atgatggccg
      361 gggtgccggt ggtgtccacc cccactggtg ccgcgctcga cgccatcgag gacggggtga
      421 gcgggatcct cgtgaaagaa cgctccggga agtcactggc cgaaggtgtg gaacgcctgc
      481 tccgcatgga cggcccactc ctgggcagga acgggcgggc cattgcgctc cgtctgttcg
      541 atctcgaggt gatgtaccgg gggtacaagg atctttatct gggcatgctc caacttccgg
      601 gaacggagcg ccgcgatcca gccacagccc catgagcgac ctggtctccg tgatcgtacc
      661 cgcgtacaac gcggggccat ggatcggcga agccgtgcga tcggtactgg cccagaccca
      721 tcgccatgtg gaggtcatcg tggtgaacga tggttccacg gatgataccc ttgagcgagt
      781 acttgagatc cctgaccccc ggctgcgcgt ggtggaccag gccaacgccg gggtgagtgc
      841 agcgcgcaac atggggctgg cgctggccaa gggcgacctg atccatttca tggacgcgga
      901 cgatgcgatg ctgccggaga acctcgccgt aaagatccaa ggattgaagg atcatggtac
      961 tgattgggtg tacggcgacc tgttgatctg cgatgcggac ctcaagccca cgggagagcg
     1021 gctccgtggg accgatgggg acgtgacgcg caccgtactg ctgggggcca ctaccgcggt
     1081 cccaacgcca tgcagcaacg tgctggcccg taggcggtgc tatgatgatg ggatccggct
     1141 ggacgagcac ctttccaacg cggccgacca ggacctggcc atccacctcg cacagcgcca
     1201 cacgtacaag cggctcaacg tcgccctgaa cctctaccgc atcgtaccgg gaagcatgag
     1261 caagagcatc ccgttgttcg aacgcgacca cctgcgcctc ttccgcaagg ccgatgccgc
     1321 ggggctgctg caagggacgc tgttccgccg gcggtgcctg gcgaacctgt attgggcgat
     1381 cgggggcagc tggtggaaat tcaccgatga acgagcgcgg gcgttgaggt tcttcctgaa
     1441 ggccgtgatg agccatccgg ccgtattggt acgtcctgta cgaaagcggc tggtcttccg
     1501 atgaaccact tgatcagcgc ctgcgagggc ccattgccgt gagcatccag cgcagcatcc
     1561 tacacacctt cctggcgcag gtgcccaccc tggccctctt cttcctggcc agtgtgctca
     1621 tgacaaacgt gctcggggat gatgggcggg gcgcgttcgc attgatgcag aacctcgtca
     1681 ttttcatgat gatgggactc gggctcaact tctccctggg gttgatgtac cacacggcac
     1741 gcagcggtgg cgatcagcgc gttccggtcg gcatggcctc ctcgttgttc ctcgtgaacc
     1801 tggtcgcggt gccatcgatc ctcgggatca tcatcttttc cgacgcgctg cgcggcatct
     1861 tcttccccgg tcggctggcg catccgatct acttcgctta tgtggccgtc tcggtgctca
     1921 tgggccaggt gatcaccttc gtgagcgcca tggtacaggg gctcaagctc ttcaccatcc
     1981 tcaaccggat gagcatcgtt accgcggcgt tcagctgcac gggcttcgtg ctcatctggt
     2041 gcttccgcgg gatgatcgat ccggcgaaca tcctgccgta cacattgctg gtcaccgtgt
     2101 ccggggtcac catgcaggca gtgatctggt ccatcattta cgtacggaag atcggcctcc
     2161 cccccgttcc gttgcgcgac tggaccgtga tgcggccttt cctcgcgttc tcgctgatgg
     2221 gctacctgac caacatgatc aacctggtga actaccgcct ggacatctgg atcgtggacc
     2281 agttcagcgg aacggcgaac ctgggcctgt acgccgcagc ggtgggtgtg gggcagttgt
     2341 tcttcaacat ccccgagccc ctgtcccggg tagtgcaacc ctacctgtat tccagcaacg
     2401 acgatgggat gatggaacgg ttccgggtga tcgcacgact gaatttcacc gcagtgacgg
     2461 tgctgtgcat attcacggcg atcatcgctc cctggctcct gccactgatg tacggtcccg
     2521 atttctcagg atcggtcacg gcactccgcc tgctgctccc gggcatcgcg ttctgcgggg
     2581 cgtacaaatt gctcgccgtg gtgctcatcc aacggggcct gctccggttc aatctgtacg
     2641 gttccgtcat cgccgctctg accacggtcg tgctggacct ggtcctgatc ccggctatgg
     2701 gcatcaccgg cgcctccatt gccagcagta tcgcctacc
//
LOCUS       DAOQOC010000317         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20565_length_2738_cov_4.095788, whole genome shotgun sequence.
ACCESSION   DAOQOC010000317 DAOQOC010000000
VERSION     DAOQOC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20565_length_2738_cov_4.095788"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PL106_06130"
     CDS             <1..318
                     /locus_tag="PL106_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNA32682.1"
                     /translation="DFLLTHSEAVQTDLANHFTKDKSVMLRQLDEMERAGWIERQMDP
                     SDRRRKNLVVTKAGLEVHRKVSRMSAKVFAKVLEGMNEKEVDTCLKVLGHMNGIANNT
                     DPE"
     gene            315..1640
                     /locus_tag="PL106_06135"
     CDS             315..1640
                     /locus_tag="PL106_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA32683.1"
                     /translation="MKRMSTIGATALLLAHAFAQQAITLRQAIDHGRTHSPAMAIATN
                     GIRRADTQFHEALAGYLPQVNGTGQFDDNLKRQTTILPAGTFGPEPVPVRFGTQFNTN
                     LSVQAEQMLVDVSQINALQANVPNRKMAALKLQQAEEQLVYDVCRAYMQAQVLEQQVK
                     LLEENLKQYDELVPILRLRLEKGVVQQLDADRVEVTRRNIGSQLTVARANQEVAIAQL
                     KRLIGMPLNEAITLADPVPTATDMRQPGPAPFAVANLSGAKYLEQALLAQAVDLRRKR
                     NAYVPTLSAYGRYGAMAQGNDLEKSYDSWYDYAAIGLKLNVPIFSSFRRLSQVKLSSI
                     ALDDLREQQRDALLGYELDHRNADTRLLASTSTAQNDEQNLRLAERVFANTNLQYQQG
                     LASLSDLLNADYQLKEARNNWTTSLLNRSIAVIDLERAKGTLLEYVNTL"
     gene            1685..>2738
                     /locus_tag="PL106_06140"
     CDS             1685..>2738
                     /locus_tag="PL106_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA32684.1"
                     /translation="MILPLVLLVLALGIGFKLAANKKKLDAAKQPVDRSAFAFPVNTI
                     TTVRDTLTGDFSVPGVLEPVDHAKVMVQAQGKLASLNVVLGSRVTRGQVLGSLDVAQK
                     QLELQAAELQLEKLGKDDQRYRELVAGKAAPESSFDDIHFNFESQKVRVEQIEQQLRD
                     AQVVSPVGGIVVAKNVEIGEFVGANTAVVEVVDVGRLKAMVRVSERDAYRIKVDDAVS
                     VTCEVFPGETFQGTVTFVSPRGDASHNYEVEVAVRNEKAHPLKSGTFINVYFRTGVAG
                     DMIAIPKNALAEGMKNPYVYVVKGDTTGMRAERRPITLGREIGDRVEVAQGLLPGETV
                     VVSGQLNLVEGSKVRVT"
BASE COUNT          603 a          823 c          884 g          428 t
ORIGIN      
        1 gacttcctgc tcacccacag cgaagccgtc cagacggacc tggccaatca cttcacgaag
       61 gacaagagcg ttatgctgcg gcagctggac gagatggagc gcgccggttg gatcgagcgg
      121 cagatggacc ccagcgaccg ccgccgcaag aacctggtcg tgaccaaggc gggactcgaa
      181 gtgcatcgga aggtgagcag gatgagcgcc aaggtcttcg cgaaggtgct ggaaggcatg
      241 aacgagaagg aggtcgacac ctgcctcaag gtgctcggcc acatgaacgg gatcgccaac
      301 aacacggatc cggaatgaaa cgcatgagca ccataggggc cacggcgctg ttgctggccc
      361 atgcattcgc ccagcaggcg atcacgctcc ggcaagccat cgatcacggg cgcacccaca
      421 gcccggccat ggcgatcgca acgaacggga tccgccgggc cgacacccag ttccacgagg
      481 cgttggcggg ctacctgccc caggtgaacg gtacgggcca gttcgatgac aacctcaagc
      541 gccagacgac catcctgccc gcggggacgt tcggcccgga accggtgcct gtccgtttcg
      601 ggacacagtt caatacgaat ttatcggtac aggctgaaca gatgctggtc gacgtgtcgc
      661 agatcaatgc cctccaggcc aacgtgccca atcggaaaat ggcggccctg aaactccagc
      721 aggccgagga gcaactggtg tacgatgtgt gccgcgcata catgcaggca caggtcctgg
      781 agcagcaggt caagctgttg gaagagaacc tcaagcagta cgatgaactg gtgcccatcc
      841 tgcggctccg cctggagaag ggagtggtcc agcagctcga tgcggatcgc gtggaggtca
      901 cccggcggaa catcggctcg caactcaccg tggcccgtgc caaccaggag gtcgccatcg
      961 cgcagttgaa acggctgatc ggcatgccgt tgaacgaggc catcaccctc gccgatccgg
     1021 tgcctaccgc taccgacatg cggcaacccg ggcctgcccc gttcgccgtg gcgaacctgt
     1081 ccggcgcgaa gtaccttgaa caggcactgc tcgcccaggc ggtggacctg cggcgcaagc
     1141 gcaacgcgta cgtgcccacc ctgagcgcgt atggccgtta cggggcaatg gcccagggca
     1201 acgatctgga gaaaagctat gacagctggt atgattatgc cgccatcggt ctcaagctga
     1261 acgtgcccat cttcagcagc ttccgacggt tgagccaggt caagctgagt tcgatcgcct
     1321 tggacgacct ccgcgagcaa cagcgcgacg ccctgctggg ctacgagttg gaccaccgca
     1381 acgccgacac gcgcctgctg gccagcacca gtaccgcaca gaacgatgag cagaacctgc
     1441 gactggcgga gcgcgtcttc gccaacacca acctgcagta ccagcagggg ctggcctcac
     1501 tcagcgatct gctgaacgcc gattaccaac tgaaggaggc ccggaacaac tggaccacct
     1561 ccttgctcaa ccgctccatc gcggtgatcg atctggaacg tgccaagggc accctgctcg
     1621 aatatgtgaa cacgctctga ccggtcaagg ccaccccaag atatcaacta ccatgaatcg
     1681 caaaatgatc ctcccgctgg tgctgctcgt actggccctt ggcatcggct tcaagctggc
     1741 cgcgaacaag aagaagctcg atgcggccaa acaaccggtc gaccgcagtg ccttcgcgtt
     1801 cccggtgaac accatcacca ccgtgcgcga caccctgacg ggcgacttca gcgtgcccgg
     1861 cgtgctcgag cccgttgacc acgccaaggt gatggtgcaa gcacagggca agttggccag
     1921 cctgaacgtg gtgctcggct cgcgtgtgac caggggtcag gtgctcggca gccttgatgt
     1981 ggcacaaaag cagttggagc tgcaagccgc ggaactgcaa ctggaaaagc tgggcaagga
     2041 cgatcaacgc taccgagaat tggtggccgg aaaagccgct cccgaaagca gcttcgatga
     2101 catccacttc aatttcgaga gccagaaggt gagggtggag cagatcgaac aacagctccg
     2161 cgatgcccag gtggtcagcc cggtgggcgg tatcgtggtc gccaagaacg tggagatcgg
     2221 tgagttcgtg ggggccaata ccgccgtggt ggaagttgtg gacgtgggcc gcctgaaggc
     2281 gatggtgcga gtgagcgagc gtgatgcgta ccggataaag gtggacgatg cggtgagtgt
     2341 gacatgcgag gtgttccccg gcgagacctt ccaaggaacc gtgacgttcg tgagcccacg
     2401 gggcgatgcc agtcacaact acgaggtgga ggtggccgtg aggaacgaga aggcgcaccc
     2461 gttgaagagc ggtaccttca tcaatgtgta tttccgcacg ggcgtcgccg gtgatatgat
     2521 cgccatcccg aagaatgcgc tggctgaagg catgaagaac ccgtacgtgt acgtggtgaa
     2581 gggcgatacg accggtatgc gggccgaacg acggccgatc acgctgggcc gcgagatcgg
     2641 cgaccgcgtg gaagtggccc agggcctgct gcccggggag accgtggtgg tgagcggcca
     2701 actgaacctg gtggaaggaa gcaaggtgcg tgtgacca
//
LOCUS       DAOQOC010000318         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_20885_length_2714_cov_3.570891, whole genome shotgun sequence.
ACCESSION   DAOQOC010000318 DAOQOC010000000
VERSION     DAOQOC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20885_length_2714_cov_3.570891"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PL106_06145"
     CDS             complement(<1..279)
                     /locus_tag="PL106_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32685.1"
                     /translation="MSTGATIKSSTTKGATDAMKKVIGHWAFWMVLAVLVLIIAWKLK
                     GKVGQWWTNLWKEDRTAPNDYTPNAEDIARVKGIAQDLYTGIHSAYASG"
     gene            complement(276..770)
                     /locus_tag="PL106_06150"
     CDS             complement(276..770)
                     /locus_tag="PL106_06150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA32686.1"
                     /translation="MKPRMLWIAILILVVLVAVAVRMRKILLPAMLPTSWLWPVKGHS
                     TVSSPFGSRTAPTAGASTDHNGIDIPAPVGTPVQAPWNGTVRQVYWNSAGGNQLIIDH
                     DDGRVSGYAHLDTTAVVAGQKVSRGETIATVGSTGHVTGPHLHFTARPSKSAPHIDPL
                     DLYA"
     gene            complement(767..1006)
                     /locus_tag="PL106_06155"
     CDS             complement(767..1006)
                     /locus_tag="PL106_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32687.1"
                     /translation="MAMFSLGSDSIGFGAITQAIAMRSLGKDYHERAQALQGAQLELA
                     EHQLQQRTQTIRIAIVVLAVVVIVVVVKLTRRKAK"
     gene            complement(1010..1558)
                     /locus_tag="PL106_06160"
     CDS             complement(1010..1558)
                     /locus_tag="PL106_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32688.1"
                     /translation="MSQVSDYTTLDPWGYWPASEDRTAVDMARVIAQDPVQAIPLVVQ
                     NNPGAVVANWSTATGAGFGMATTPEHIIADLTRRWEEGDRKGVQDILAVQWIPGADAE
                     QDKAVQQLRDQYNMPEAGLGQLWNKLLAIGADDAATITTTGSDTPKTPSKRKKYVRYA
                     LIGLAALALVAIAVYLYKQHRR"
     gene            complement(1555..2142)
                     /locus_tag="PL106_06165"
     CDS             complement(1555..2142)
                     /locus_tag="PL106_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32689.1"
                     /translation="MDIKTFRERVSTEPSFLVSYIVANNPEAVMENLQAEGQIVNDEA
                     DIFPLLNELLAEGKEKVVRRVLSVPVNTDNMSAAEMAVLMDITKGRQQANGMLKKSGE
                     SKEEDSDSGTGSEGGSFWNSAAFGSLLQGVFSLGAGYLSSQGLYNVPTGATAGRTAQD
                     EAAKKARTTRYILIGVAVLAVIAIGFFVWKSQSSK"
     gene            complement(2211..>2714)
                     /locus_tag="PL106_06170"
     CDS             complement(2211..>2714)
                     /locus_tag="PL106_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32690.1"
                     /translation="PISANGALAKAPGPAGNNAPLSYITYTIANGGAALTYVIGDPTG
                     MVQGAFNPAIAWSQPTAVTGGAVAAVQASFMSAPVSIKGINYIVTTAPQYAQTLRYCG
                     ADRDGRINGQPVQIMPYLRNNQFITTRQTIMFDEPYGFDEFHAFTLDVLANETVTISL
                     LLDANAA"
BASE COUNT          424 a          847 c          880 g          563 t
ORIGIN      
        1 tcccgaagca taggccgaat ggatacccgt gtacaggtcc tgggcgatgc ctttcacccg
       61 cgcgatgtcc tcggcgttcg gggtgtaatc gttcggcgcg gtgcgatcct ccttccacag
      121 gttcgtccac cactgcccca ccttgccctt cagcttccaa gcgatgatga gcaccagcac
      181 ggccaggacc atccagaagg cccagtggcc tatcaccttc ttcatggcat ccgtcgcgcc
      241 tttggtggtg gaggatttga tggttgctcc ggtgctcatg cgtagaggtc cagggggtcg
      301 atgtggggcg cggactttga aggcctcgcc gtgaaatgga ggtgcggccc ggtgacgtgg
      361 cctgtgcttc ccacggttgc gatggtctcg ccccggctca ccttctggcc ggccacaacc
      421 gcggttgtat cgaggtgggc atagccgctc acccgcccgt cgtcgtggtc gatgatgagc
      481 tggttgcctc cggcgctgtt ccaatagacc tgtcgcaccg tgccgttcca gggagcttgc
      541 accggtgtac cgaccggtgc cggaatgtcg atgccgttgt ggtcggtgct ggccccggcg
      601 gtgggcgctg tgcgcgagcc gaacggtgag gacacggtgg aatgcccttt cacgggccaa
      661 agccaggagg tcggcagcat ggcaggcagc aggatcttgc gcatgcggac cgccacggcc
      721 accaggacca cgaggataag aatggcgatc cagagcatgc ggggtttcat ttggccttgc
      781 ggcgtgtgag tttcaccacc accacgatca ccaccaccgc gagcacgaca atggcgatgc
      841 ggatggtctg ggtgcgctgt tggagctggt gctcggccag ttcgagctgc gcaccctgga
      901 gcgcttgggc ccgctcatgg tagtccttgc ccaggctgcg catcgcgatc gcttgggtga
      961 tcgcgccgaa gcctatgcta tcagagccca gggagaacat ggccatacct cagcggcggt
     1021 gttgcttgta caggtacacg gctatcgcca ccagagccaa tgccgccagc ccgatcaggg
     1081 catagcgcac atacttcttc cgcttgctcg gcgtcttcgg cgtatcggag ccggtggtgg
     1141 tgatggtggc ggcatcgtcc gcgccaatgg ccagcaactt gttccagagt tgccccaggc
     1201 cagcctccgg catgttgtac tggtcgcgga gctgctgtac ggccttgtcc tgttcggcat
     1261 cggcaccggg gatccactgt acggccagga tgtcctgcac acccttgcgg tctccttctt
     1321 cccagcgtcg ggtgaggtcg gcgatgatgt gttcaggcgt tgtggccatg ccgaagcctg
     1381 cgccggtcgc agtgctccag ttggccacca cggcacccgg attgttctgc accacgagcg
     1441 gtatggcctg caccgggtcc tgcgcgataa cacgcgccat atccacggcg gtcctgtcct
     1501 cggatgcggg ccaatacccc caggggtcga gtgtggtgta gtccgacacc tggctcattt
     1561 gctgctctgg gatttccaca cgaagaagcc gatggcgatc accgcaagca cggccacgcc
     1621 gatgaggatg tagcgcgtgg tcctggcctt tttcgcggcc tcgtcctgcg ccgttcgccc
     1681 tgcggtagca ccggtgggca cgttgtatag cccttggctg ctgaggtatc ccgctcccag
     1741 ggagaacacc ccctggagga gcgagccgaa agctgcgctg ttccagaagc tacccccttc
     1801 cgagccggta ccggagtccg agtcctcttc cttgctttcc ccggacttct tcagcatgcc
     1861 gttcgcctgc tggcggccct tggtgatgtc catgagcacc gccatttcgg ccgcgctcat
     1921 gttgtcggtg ttcaccggta cagacagcac ccggcgcacc accttctcct tcccttcggc
     1981 gagcagctcg ttgagcagcg ggaagatgtc cgcttcatcg ttcacgatct gcccttcggc
     2041 ctggaggttc tccatgaccg cttcggggtt gttcgccacg atgtaggaga ccaggaagct
     2101 cggttccgtg cttacccgct cccggaatgt tttgatatcc atggcagggt gtggtgtggt
     2161 ggatgcgggg gcaaggccct accctcgccc ccttgcatcc ggtgccgcgc tcaggccgcg
     2221 ttggcgtcca ggagcagcga aatggtgacc gtctcgttcg cgagcacgtc cagcgtgaag
     2281 gcgtggaact catcgaaccc gtacggctca tcgaacatga tggtctgccg ggtggtgatg
     2341 aactggttgt tgcgcaggta cggcatgatc tgcacaggct ggccgttgat acggccgtcg
     2401 cgatccgcac cacagtagcg caaggtctgc gcgtattgcg gggccgtggt cacgatgtag
     2461 ttgatgccct tgatgctcac cggagcgctc atgaagctgg cttgtaccgc ggccacggca
     2521 ccaccagtta cggcggtggg ctgcgaccag gcgatcgccg gattgaacgc tccctgcacc
     2581 atgcccgtgg ggtcgccgat cacataggtg agcgcggcac caccgttcgc gatggtgtag
     2641 gtgatgtagc tgagcggggc attgttcccg gcagggccag gagccttggc caacgcgccg
     2701 ttggcactga tggg
//
LOCUS       DAOQOC010000319         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21012_length_2705_cov_4.290943, whole genome shotgun sequence.
ACCESSION   DAOQOC010000319 DAOQOC010000000
VERSION     DAOQOC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21012_length_2705_cov_4.290943"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PL106_06175"
     CDS             <1..658
                     /locus_tag="PL106_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNA32691.1"
                     /translation="THEFIRNGSRGHAYTPIIGSGYNACVLHYITNDMVCNDGEVILM
                     DFGCEYGGYASDLTRSIPVNGRFTKRQKDVYNAVHRVQQEATQLLRPGTLLADYHKEV
                     GRIMESELIGLGLLDKNDVAKQDPEKPLYKKYFMHGTSHFLGLDVHDVGLWNEMIQPD
                     MVFTVEPGIYIREEKLGIRLENNILVTRDKPIDLFKDIPVEADAVEEMMASKGKKVMA
                     "
     gene            complement(750..>2705)
                     /locus_tag="PL106_06180"
     CDS             complement(750..>2705)
                     /locus_tag="PL106_06180"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32692.1"
                     /translation="RNCYRTVVLTPSVLLIFSMHAQSPGGHATNLRAWYKANANAYRN
                     SGVNLCTNGQTVRQWNDQSGNALTLTQATSGQRPTWYDGSGSVFCNYNPSIRFSDHYL
                     QRTGSGGSPGVLVSGTTYNQLNLYAVYYDNNANNFDWLFNSGGNNGFNRVSLSMNWSG
                     SSDFDCDVTSTTNRITTSTSSVLPVAHTNIIAVKSDNTGIYGGSPASSNKIQAFCNGT
                     PDAARTTHTPIAVNNNITQIGDNELVTTDALDEPFGGDAMEFILYTGPVSQVVHQRIE
                     TYLALKYSVTLTSHDYFNTAGTNIFALSGGYTQDIIGVIRDDGQGLYQKQSRQQDDSA
                     RIYIGTLSATNVGNAATMAGDRQSLVIGHNGAAMRSSVATSGEIPGGAGLISRIAREW
                     KVTNTAYTGTFSLAFKLNTSPIVPGDLRLLVDDDGNFTNASVNGNPEGLTFTYSGGIV
                     TVTGISTTLVPTNSTRYITLASVANSTPLPIELLYFHALPADPDAVQLLWSTASERDN
                     AFFEVQRSRSGVTYETIGSVDGNGNSTTTIAYEFNDPTPYPGPNYYRLKQVDLDGTST
                     FTEVRSVTIGADGPIHVYPNPAVDQVRIAGLLQGSTDPVEVYNAAGERMPFAPEDMNE
                     GLLDVSTLPKGIYLVRIGDQVARVVKE"
BASE COUNT          530 a          774 c          849 g          552 t
ORIGIN      
        1 cacccacgag ttcatccgca acggctcacg cggtcacgct tacacgccga tcatcggtag
       61 cggatacaac gcctgtgtgc tgcactacat caccaacgac atggtgtgca acgatggcga
      121 ggtgatcctg atggacttcg gctgcgagta cggcggctac gccagcgacc tcacgcgaag
      181 tatccccgtg aatggccgtt tcaccaaacg ccagaaggac gtgtacaacg ccgtgcatcg
      241 cgtgcagcag gaggccaccc aactgctgcg ccccggcacg ctgctcgccg actaccacaa
      301 ggaggttggc aggatcatgg agagcgaatt gatcggcctg ggcctgttgg ataagaacga
      361 cgtggccaaa caggatcccg aaaaaccgct ctataaaaaa tacttcatgc acggcaccag
      421 ccacttcctg ggcctggatg tgcatgatgt gggcctgtgg aacgagatga tccagccgga
      481 catggtcttc accgtggagc cgggcatcta catccgcgag gagaagctgg gcatccgcct
      541 ggagaacaac atcctggtga cacgcgacaa gccgatcgac ctgttcaagg acatccctgt
      601 ggaggccgat gcggtggagg agatgatggc gagcaagggg aagaaggtga tggcctaagt
      661 cccttggtac caccatgggc gaacacggcc ctgagccttg cggcacgaat tacgatgcgt
      721 ggccatccga ccagcgcatg ccacacgtgc tattccttca cgacacgcgc cacctgatcg
      781 ccgatccgaa cgaggtagat ccccttcggc aatgtgctga catcgagcag gccttcgttc
      841 atgtcctcgg gcgcgaaggg catccgttca cccgctgcgt tgtacacctc caccgggtcg
      901 gttgagcctt gtagcaggcc cgcgatccgc acctggtcca cggccgggtt cgggtacacg
      961 tgtatcggcc cgtcagcgcc gatggtcaca ctgcgcacct ccgtgaaggt ggacgtccca
     1021 tccagatcca cttgtttcaa gcggtagtaa ttcgggccgg gatagggcgt ggggtcgttg
     1081 aactcgtacg cgatggtggt ggtggaattc ccattgccgt ccaccgaccc gatggtctcg
     1141 taagtgacgc ccgacctgct gcgctgcact tcgaagaacg cgttgtcccg ctcgctggcc
     1201 gttgaccaga ggagttgcac cgcatccggg tcggccggta gcgcatggaa atacagcaac
     1261 tcgatgggaa gcggcgtgct gttggccacg gaggcgagcg tgatgtagcg tgtggagttc
     1321 gttggaacga gtgtcgtgga gatgcccgtg accgtcacga taccgccgga ataggtgaat
     1381 gtgagtcctt ccgggttgcc attgacactg gcgttggtga agttgccgtc atcgtcaacc
     1441 aacaggcgca ggtcgcccgg tacgatcggc gatgtgttga gcttgaatgc caggctgaag
     1501 gtcccggtgt atgcggtatt cgtcaccttc cactcccggg caatgcggct gatcagcccg
     1561 gcaccaccgg ggatctcgcc tgaggtcgcg acactcgagc gcatggccgc accattgtgc
     1621 ccgatcacca gcgattgacg atcgcccgcc atggtggccg cgttgccgac gttggtagcg
     1681 gacagggtgc cgatgtaaat gcgagcgcta tcatcctgct ggcgcgattg tttctggtaa
     1741 aggccctggc cgtcatcccg gatcacgccg atgatgtctt gcgtgtagcc accgctcagg
     1801 gcgaagatgt tcgttcctgc cgtgttgaag tagtcgtggc tggtcaacgt gaccgagtac
     1861 ttcaacgcga ggtaggtctc gatgcgctgg tggaccacct ggctcacggg ccccgtgtac
     1921 aggatgaact ccatggcatc gcccccgaag ggctcatcaa gcgcatcggt cgtgaccagt
     1981 tcgttatcgc cgatctgggt gatgttgttg ttcaccgcaa tgggcgtatg ggtcgtccgc
     2041 gccgcatcgg gagtgccatt gcaaaaggcc tggatcttgt tcgagctggc cggagagcct
     2101 ccgtaaatac cggtattatc cgatttcacg gcgatgatgt tcgtatgcgc gaccggtaga
     2161 acactgctcg tgctggtggt gatcctgttg gtggtggatg tcacatcgca atcaaagtcc
     2221 gatgatccgc tccagttcat ggacagggat acccggttga agccgttgtt cccaccggaa
     2281 ttgaacagcc agtcgaagtt gttcgcgttg ttgtcgtagt acaccgcata cagattcaac
     2341 tggttgtacg tggtcccgct gacgagcacc ccgggggagc caccgctacc ggtgcgttgc
     2401 aggtagtggt ccgagaagcg gatcgaaggg ttgtagttgc agaagaccga gcccgagcca
     2461 tcgtaccagg tggggcgctg accgctggtg gcctgggtca gggtcaaggc atttcccgac
     2521 tggtcattcc actgccgcac cgtctgaccg ttggtacaca gattgacccc tgagttcctg
     2581 tatgcgttcg cattggcctt gtaccaggcg cgcaggttgg tcgcgtggcc gcccggactt
     2641 tgggcgtgca tcgaaaagat gagcagtacg gaaggggtca gaacaaccgt tctgtaacaa
     2701 ttgcg
//
LOCUS       DAOQOC010000320         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21032_length_2704_cov_3.094375, whole genome shotgun sequence.
ACCESSION   DAOQOC010000320 DAOQOC010000000
VERSION     DAOQOC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21032_length_2704_cov_3.094375"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PL106_06185"
     CDS             complement(<1..907)
                     /locus_tag="PL106_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32693.1"
                     /translation="MENAIITTLIGTFAIGSLGLLATLPEPPGSILAKPVDEASDVMD
                     TLQRLVTYDLRVADGSRYDLNDSLFWSIEDRSGERRFISDVRVLRQVFPDTGRYVVNS
                     FRHMRVIARDTFVVVDGERFDIEWPDAYPVVGDEMEFGDYSTGALKDGTLWHITGDST
                     DTTWNEPNFTWRPVKAGAYSANVRVQFGSGLEKDTTWHFLVSAPAPVKPDPDLPRKEV
                     VVRKKRRSAPPPRRAPPPPAPNAAFGREDVRFTGRLRMEIESQDRKKITDYRDSEVAF
                     LFTPPVNCVLDRFSYYTQPTGGKGQI"
     gene            complement(922..1464)
                     /locus_tag="PL106_06190"
     CDS             complement(922..1464)
                     /locus_tag="PL106_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32694.1"
                     /translation="MRPLNTRERRVQFFRFLALFLLAIAPFILLTWLFGRVDHVENEF
                     LRSQYETKRADGAVAAKYREQLIKLERSTGGLKDYVVTKSGTMAKLDFNDNGTLTDHI
                     GKVKSDLENFENESRESDSTLISIAKDLESVATNLQNAYIEAAEEVAKKNEELSEMKK
                     DLDKAKTERDEFETRYNQVK"
     gene            1658..1924
                     /locus_tag="PL106_06195"
                     /pseudo
     CDS             1658..1924
                     /locus_tag="PL106_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            1997..2482
                     /locus_tag="PL106_06200"
     CDS             1997..2482
                     /locus_tag="PL106_06200"
                     /inference="COORDINATES: protein motif:HMM:NF026980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin glutamine deamidase domain-containing
                     protein"
                     /protein_id="HNA32695.1"
                     /translation="MLGALWQYVKNIFDPPTDDPRAPAIIVAQVNPLDGGINCGHIID
                     AVIARLDGSSPYAITATTQRDGSWEEIETRHGTTFTWGKSFQQVYAEVKAGGPGTTHI
                     VGMAGKKEAHVVVITNHNGTPVILEGQGGGAVIDSADEAAARYDPGFYGDGFTVGSAP
                     L"
     gene            2486..>2704
                     /locus_tag="PL106_06205"
     CDS             2486..>2704
                     /locus_tag="PL106_06205"
                     /inference="COORDINATES: protein motif:HMM:NF026905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB domain-containing protein"
                     /protein_id="HNA32696.1"
                     /translation="MITFEQARDIALAELARALPKYDFVLLDDETVTMPYGWIFWYNT
                     RKYLETGDIMASTPGNAPFVVERSDGRIH"
BASE COUNT          507 a          838 c          785 g          574 t
ORIGIN      
        1 tgatctgccc tttgccgccg gtcggctgtg tatagtacga gaaccggtcc agcacgcaat
       61 tgaccggtgg ggtgaacagg aatgcgacct ccgaatccct gtagtccgtg atctttttac
      121 gatcctgtga ttcgatctcc atgcggagcc tgccggtgaa gcggacatcc tccctgccga
      181 aagcggcgtt gggagcgggc gggggtgggg cccgtcgggg aggcggtgca ctccgccgct
      241 tcttccggac caccacttct ttcctgggca ggtccgggtc gggcttgacc ggggcgggtg
      301 cgctcaccag gaaatgccat gtggtatctt tttccagtcc tgacccgaac tgcacacgta
      361 cgttggcgga gtacgcgccc gccttgacgg gtcgccacgt gaagttgggc tcgttccatg
      421 tggtgtccgt gctgtcgccc gtgatgtgcc acagggtccc gtccttgagc gcaccggtgg
      481 aatagtcgcc gaactccatc tcgtcgccta ccaccgggta cgcatcgggc cattcaatgt
      541 cgaaccgctc accatccacc accacgaagg tgtcccgagc gatcacccgc atgtgcctga
      601 aactgttcac cacgtaacgc ccggtgtccg gaaagacttg gcgcagcaca cgaacgtcgc
      661 tgatgaacct gcgttcgccg gaacgatcct cgatcgacca gaacagcgaa tcgttcaagt
      721 cataccgtga accatccgcc acgcgcaagt cgtacgtgac caggcgttgc aacgtatcca
      781 taacatccga tgcctcatcg acgggcttcg ccaaaatgga acccggaggc tcaggcaatg
      841 tggccaacag tcccaacgaa ccgatagcga aggtgcctat cagtgtggtg ataatggcgt
      901 tctccattcc ttcctgacgg tctatttgac ctggttgtat ctggtctcga actcgtcacg
      961 ctcggtcttg gccttatcca ggtccttctt catctccgaa agctcctcat ttttcttggc
     1021 aacttcctca gctgcctcga tgtaggcgtt ctgaaggttc gtcgcgaccg attccaggtc
     1081 cttggcgata ctgatcaatg tcgaatcgct ttcgcgggac tcgttctcga aattctcgag
     1141 atcgcttttg accttcccga tatgatcggt caaggtcccg ttgtcgttga agtccagttt
     1201 ggccattgtg ccgctcttgg tcaccacgta gtccttcagg ccacctgtgc ttctttcgag
     1261 cttgatcaat tgctccctgt acttggcggc cacggcacca tcagcacgct tggtctcgta
     1321 ctgtgaccgg aggaactcgt tctccacgtg gtccacccga ccgaacaacc aggtgaggag
     1381 gatgaacggt gcaatagcga gaaggaagag ggccaggaag cggaagaatt gtacgcgccg
     1441 ctcacgggtg ttcagtggtc tcatggatca attcgcggat cagcgaatat aacagccggt
     1501 cagaaacgga ctctgccacg tcgtggtgac cagcagggcc gtacgcacag gctccagttg
     1561 cgcgatcggc accgccgaag gctcgttcgg accttcgtcc ccaaaggccc gtggcgaact
     1621 tggcagcatg ttcatccatc ctgatccgta ttgaatcatg agtctgccag cagcccgagt
     1681 gggtgatatg catatctgcc cgatggtgac tcccgctccg gttcctgtgc cgcacgtggg
     1741 cggtcccatc ctgccgcccg gcacccctgt ggtattgatc ggtgggatgc ccgctgccac
     1801 catgggcgac atgtgtacct gcgttggtcc gcccgatgtg atcgccatgg gtgcggcaac
     1861 cgtgctgatc agcggtcgtc cggccgcgcg catgagtgat accaccatgc atggcggtac
     1921 cgttgcattc gtttttcccc ccgttctgat cggaggggcc ggaaccgcca gtgttacgcc
     1981 cccgggaccc acgaccatgc tgggggcgct ctggcagtac gtcaagaaca ttttcgaccc
     2041 acccacggat gatccccggg caccagccat catcgtggcc caagtgaacc ccttggatgg
     2101 tggcatcaat tgtggtcaca ttatcgatgc ggtgatcgcc cggctcgatg gatccagccc
     2161 gtacgccatc accgccacca cgcaacggga tggttcctgg gaggagatcg agactcgcca
     2221 cggcaccacg ttcacctggg gcaaaagctt ccagcaggtg tatgccgagg tgaaggccgg
     2281 cgggcccggc accacgcaca ttgtgggcat ggccggcaag aaggaggccc atgtggtggt
     2341 gatcaccaac cacaacggta cccccgtgat cttggaaggg cagggcggtg gggccgtcat
     2401 cgattcggcc gacgaggccg ccgcccgata tgatcccggt ttctatggtg atggtttcac
     2461 cgtaggttca gccccgcttt gacgcatgat cacctttgaa caggcccgtg atatcgccct
     2521 ggccgaactg gcccgggccc tgcccaagta cgacttcgtc ctgctggacg acgagaccgt
     2581 gaccatgccc tacggatgga tcttctggta caacacccgg aaatacctgg aaacagggga
     2641 catcatggcg tccacccccg gcaatgcgcc cttcgtggtg gaacgttccg atggccgcat
     2701 acac
//
LOCUS       DAOQOC010000321         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21041_length_2703_cov_3.353474, whole genome shotgun sequence.
ACCESSION   DAOQOC010000321 DAOQOC010000000
VERSION     DAOQOC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21041_length_2703_cov_3.353474"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2703)
                     /locus_tag="PL106_06210"
     CDS             complement(<1..>2703)
                     /locus_tag="PL106_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32697.1"
                     /translation="FVLGGLLTALTGADPGGSWTAPGGGPHTGLVDPAVDAQGVYVYT
                     LAAMAPCLGDQSEVTVTITPAPVAGTDGSVTVCDSGAPVVLLGVLSGAQPGGSWTGPG
                     GAVFSGTYDPASDVSGPYTYTLVGAPPCMAAQAVVTVTETGSPQAGADGTITVCSNGA
                     TTGLFAALNGADPGGTWTAPGGAVHSGSFDPGTDPVGVYTYTLSATPPCTGDQSQVTV
                     TVQAAPDAGNDGALTVCDLGGASALFTALTGADAGGVWTAPGGGAHSGSFDPTTDAPG
                     IYTYTVIGVSPCATDQAAVSVTTTGSPDAGTDGALTLCSTGVPTGLFAALNGADPGGT
                     WTAPGGAVHSGSFDPGVDPPGVYTYTLTAAPPCAGDQSQVTVTIQAAPEAGNDGALTV
                     CDQGGASALFAALTGADAGGFWTAPGGGAHSGSFDPATDASGIYTYTVNGMAPCVADE
                     AEVTVTVTGSPDAGTDGVLSLCTNGLPTMLFGALGGADAGGTWTAPGGAAHSGTLDPL
                     LDGAGTYTYTIAALAPCTSDQATVTVGIQQPPQAGVDGALPVCDQGAPGALLAALTGA
                     DAGGTWTAPGGGPHSGSYDPAMDPEGDYTYTVSGMAPCPSDQAVVTVQETGSPQAGAD
                     GTLTLCASSGTFDLFSALTGADPGGAWIGPDGMPHASIIDPGTDMAGQYTYMLAATPP
                     CTGDQSQVLVTLVAPPDPGADAAAVLCDMGQPVDLFTELSGAEAGGTWTSPGGGAHSG
                     TYDPITDAPGDYTYTVVGTAPCGMAQAVVTVTETSAPDAGTSTVLDLCSTAGPVSLLV
                     QLGGADPGGSWTGPGGAPHSGTFVPALDTPGDHTYTIAGTAPCPSASAVVTVQVEAAP
                     DAGVDGGFTVCSGDAPFDLLVLLGGTPDPDGTWSDVLNAPFNGV"
BASE COUNT          480 a          934 c          873 g          416 t
ORIGIN      
        1 aagaccccat tgaacggcgc attgagcaca tccgaccagg tcccatcggg atccggcgtt
       61 ccaccaagca gaaccaacag gtcgaacggc gcatcaccac tgcacacggt gaagccaccg
      121 tccacccccg catcgggcgc agcctccacc tggaccgtta ccacggcact ggccgatggg
      181 cagggcgctg tacctgcaat ggtgtatgta tgatcaccgg gcgtgtcgag tgcgggaacg
      241 aatgtgccgc tgtgcggggc tccgccgggc ccggtccagg aaccaccggg atccgcgccc
      301 ccgagttgta ccagcaagga taccggccct gccgtggaac acaggtcgag cacagtggac
      361 gttcccgcat cgggcgcgct cgtttccgtc accgtgacca cggcctgggc catgccgcag
      421 ggggctgtac caaccaccgt gtaggtataa tcgcccgggg catccgtgat cggatcgtag
      481 gtcccactgt gcgccccgcc accaggggaa gtccatgtac caccggcttc cgcaccggac
      541 agctcggtga acaggtccac gggttgaccc atgtcgcaga gcaccgcggc cgcgtcggca
      601 ccgggatccg gaggggccac caaggtgacc agcacctgcg attgatcccc cgtacaaggg
      661 ggtgtggccg cgagcatgta cgtgtactgg ccggccatgt ccgtgcccgg atcgatgatg
      721 gatgcgtgcg gcatgccatc gggcccgatc caagcacccc ccggatccgc gccggtcagc
      781 gcgctgaaga gatcgaaggt accggacgag gcacaaagcg tgagcgtgcc atccgccccg
      841 gcctgtggcg aaccggtttc ctgtacggtg acaacagcct ggtccgatgg gcagggggcc
      901 atgccggaga ccgtgtatgt ataatcccct tccgggtcca tggccggatc gtacgatccg
      961 ctgtggggac cgcctcccgg agcggtccaa gtgccgcccg catccgctcc ggtgagtgca
     1021 gcaagcagcg caccgggggc accctggtcg cacacgggga gcgcaccgtc aacaccggcc
     1081 tgtggtggct gctggatacc gaccgttacc gttgcctgat cgctcgtaca tggtgccagc
     1141 gcagcgatcg tgtacgtgta tgtacccgcg ccgtccagca acggatcgag tgtaccactg
     1201 tgcgcagcac cgcccggagc cgtccacgtt ccacccgcat cggctccgcc gagcgcccca
     1261 aaaagcatgg tcggaagccc gttggtacaa agcgacagca caccgtcggt acccgcatct
     1321 ggtgatccgg tcacagtcac ggtcacctcc gcctcatcgg cgacacaggg agccataccg
     1381 ttcaccgtgt acgtatatat cccggatgca tcggtggcgg ggtcgaacga gccgctgtgc
     1441 gcccctcctc ctggagcggt ccagaagcct ccggcatccg ctccggtgag tgctgcgaac
     1501 agcgcactgg cccccccctg gtcgcagacg gtgagagccc catcattacc ggcctccggt
     1561 gcggcttgta tcgtcaccgt cacctgcgac tggtcgcccg cgcaaggtgg tgcggcggtc
     1621 aacgtgtacg tgtacacccc ggggggatcg acccccggat cgaaggatcc gctgtgcact
     1681 gcgcccccgg gagcggtcca cgtgccgccg ggatcagctc cgttgagggc cgcgaagagc
     1741 cctgtgggca caccggtgga gcagagggtc agggcaccat cggtaccggc atccggcgaa
     1801 cctgtcgtgg tcacgctgac cgcggcctga tcggtggcac acggcgacac tccgatcacc
     1861 gtgtaggtat agatccccgg tgcatcggta gttggatcga aggaaccact gtgtgcaccg
     1921 ccacccggcg cggtccaaac gccacccgca tccgcaccgg tgagtgcagt gaaaagcgcg
     1981 ctggcccccc ccaggtcgca gacggtgagc gccccatcat tgccggcatc cggtgcggct
     2041 tgtaccgtca ccgtcacctg cgactgatcg cccgtacagg gtggagtagc actcaacgtg
     2101 tatgtgtaca cgccgacggg atcggtcccc ggatcgaagg atccgctatg cacagcgccc
     2161 ccgggagcgg tccatgtgcc gccgggatca gctccgttga gtgctgcgaa gagccctgtg
     2221 gtcgcaccat ttgagcagac cgtgatcgtc ccgtcggcac ccgcttgagg ggagccggtc
     2281 tccgtcacgg tgaccacggc ctgtgccgcc atgcacggag gtgcgccaac aagggtatag
     2341 gtgtacgggc cgctcacatc actggccgga tcataggtcc cgctgaatac tgctccacca
     2401 ggaccggtcc aactaccacc gggctgggca ccggacaaca cgccgagcag caccaccggt
     2461 gctccgctat cgcacactgt gaccgagccg tccgtaccgg ccaccggcgc gggggtgatc
     2521 gtcacggtca cctcggactg atcaccgagg cagggggcca tggccgcgag cgtatagacg
     2581 tacaccccct gcgcatccac cgcggggtcg acgaggcccg tgtgcggacc accgcccggt
     2641 gcggtccagg aacctcccgg gtccgcaccg gtcagggcgg tgagcagtcc gccgaggacg
     2701 aag
//
LOCUS       DAOQOC010000322         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21053_length_2702_cov_3.949377, whole genome shotgun sequence.
ACCESSION   DAOQOC010000322 DAOQOC010000000
VERSION     DAOQOC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21053_length_2702_cov_3.949377"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PL106_06215"
     CDS             <1..1043
                     /locus_tag="PL106_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32698.1"
                     /translation="TSTVSITAQTYPDIVISTTGNATVACPGQTASASVSGVSGGTGN
                     YTGQVWTDPDGATVTTDNSFTAVVNGDQTYTVTVTDHCGHTGTAQVTLAVEYHTPLAV
                     HLPDAAVCENGSRELIAQATGGAGGYTYAWSGFPDVDSAVTVSPTEPETYFVTVTDAC
                     GITASDNALVTIEHPVSTIVAESIGYDDFAFSTNSLPGAVQYAWEFGDGHTSTDARPQ
                     HAYSEMQVTTVTVHTWTANGCPATDTIVLTPGAQLFFPNAFTPDGDGINDFFGATGLN
                     LDEFEMLIFNRWGEQIAAITGPGAVWDGRVNGTRAPDGVYVYSFRAAGNRLQEIKGTG
                     HVTLLGDLGAAE"
     gene            1181..>2702
                     /locus_tag="PL106_06220"
     CDS             1181..>2702
                     /locus_tag="PL106_06220"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNA32699.1"
                     /translation="MRRIRTAIAAATLLPISSLGQIVLDNTITPTQAVVDVLLGQGVT
                     VSNITFNGQPGNLLNEQIGGFDATNAILGINEGVIIATGSIGNAVGPNNSGSSSLGGG
                     NFGQTDPDLVLLSGNPSINDAGILEFDFVPTGDSLKFDFIFASEEYLEFVNSVNDAFG
                     FFISGPGINGPYQNNAINIALVPGTTQPVTINDVNSTVNSTYYVDNGDGWTAPYNTNP
                     QYVQYDGHTVVMTAKTEVTCGVTYHIKICIGDASDTAWDSAVFLQAGSFTSSGQVVPE
                     LTGDLGGVGSNDSTLFEGCGIIPLVFHRYGDTTNVDTVNLIIGGTATGGVDYSPAFPV
                     QLIYQPGDTIIPWPVTVPLDADGLETITIGIAQNIVCSGQQILNEYVLYIDQYPALNV
                     VTNDVDGDCGGQYTLTPVITGGTGLAQILWDTGETTTSITVSPGVTTTYDFTVTDTCS
                     LSPVDGQITVTIPVYEPMAVEVSPLTLIDCLSNGDIAVTNVTGGDGDYDYAWTFEGA"
BASE COUNT          551 a          809 c          797 g          545 t
ORIGIN      
        1 ccaccagcac ggtgagcatt acggcccaga catacccgga catcgtgatc tccaccacgg
       61 gcaacgcgac agtggcctgc cccgggcaaa ccgcgagcgc cagtgtttcc ggtgtttccg
      121 gtggtacagg aaattatacc gggcaggtgt ggacggatcc cgatggcgcc actgtgacca
      181 cggacaactc ctttaccgcc gtggtgaacg gcgatcagac ttacacagtg acggtcaccg
      241 atcattgtgg ccacaccggt acggcccaag tgacgctggc cgtggagtac cacacgccct
      301 tggcggtaca cctgcctgat gctgcggttt gcgagaacgg aagccgtgag ctgatcgccc
      361 aggccaccgg tggtgctggt ggctacacgt atgcatggtc cggcttcccc gatgtcgaca
      421 gcgccgtaac ggtgagccct acggagcctg aaacctactt tgtaacggtg actgatgcgt
      481 gcggtatcac cgcttcggac aacgcgctgg tgaccattga acaccccgtt tccaccatcg
      541 tggcggaaag tatcggatac gacgatttcg ctttcagcac caacagcctg cccggagcgg
      601 tgcagtacgc gtgggagttc ggcgatgggc acaccagcac cgatgcccgc ccgcagcatg
      661 cctattcgga aatgcaggtg accaccgtta ccgttcatac ctggaccgcc aatggctgcc
      721 cggccacgga caccatcgtg ctgacccctg gcgctcaact cttcttcccc aatgcgttca
      781 ccccggatgg cgatggcatc aacgacttct tcggtgccac agggttgaac ctggacgagt
      841 tcgagatgct cattttcaac cgttggggcg agcagatcgc cgcgattacc ggccctggcg
      901 cggtttggga cggtcgcgtg aatggcacca gggctccgga tggtgtgtac gtgtacagct
      961 tccgtgcggc gggcaaccga ttgcaggaga tcaaaggcac gggccatgtg accttgctgg
     1021 gtgacctcgg tgctgctgag taagatgctg tatgccaagg gggtgcggca caatgagccg
     1081 accacaggtg cttggtcccg gtcctcaggc aactttgggg gcctttaatg ggtcaagcaa
     1141 gggtccggga agctatcttc acacgctttt tcaagcaccc atgcgccgta ttcgcaccgc
     1201 aattgctgcg gccacccttc tgcccatctc gagccttggc cagatcgtgc tggataacac
     1261 catcaccccc acgcaggcag tggttgatgt ccttctgggt cagggggtta cggtaagtaa
     1321 tatcaccttc aatgggcagc cgggcaacct cctgaacgag cagatcggcg gctttgatgc
     1381 caccaatgcc atcctcggga tcaacgaggg ggtcatcatc gccactggca gtatcggcaa
     1441 tgccgttggg cccaacaact cgggcagcag cagtttggga ggtggcaatt tcggccagac
     1501 cgatccggat ctggtcctgc tctccggcaa tcccagcatc aatgatgccg gcatccttga
     1561 gttcgatttc gttccaaccg gcgattcgct caagttcgat ttcattttcg cttccgagga
     1621 gtacctggag ttcgtgaaca gtgtgaacga tgccttcggc ttcttcatca gcggcccggg
     1681 gatcaatggt ccttaccaga acaacgcgat caatatcgcc ttggtccctg gtaccaccca
     1741 gccggtgacc atcaatgatg tcaactccac agtgaattcc acctactatg tggacaatgg
     1801 tgatggatgg accgctccgt acaataccaa cccgcaatat gtccagtacg acgggcatac
     1861 ggtggtaatg accgcgaaga ctgaggtgac ctgtggtgtg acttaccaca tcaagatctg
     1921 catcggcgat gcgagtgaca cggcctggga cagcgccgtt tttcttcagg cgggaagctt
     1981 taccagcagc gggcaggtgg tgcctgagct caccggggat ctcggtggtg tgggctccaa
     2041 cgacagtacg ctgttcgaag ggtgcggcat cattccactg gtcttccacc ggtacgggga
     2101 cacgacgaac gtggataccg tgaacctcat catcgggggc acggccaccg gaggcgttga
     2161 ctattccccg gcttttccgg ttcaattgat ctatcagccg ggggatacca tcatcccttg
     2221 gcccgttacg gtgccgctgg atgccgacgg cctcgaaacg atcaccatcg ggatcgcgca
     2281 gaacatcgtg tgcagtggcc agcagatcct caacgagtac gtgctgtaca tcgatcagta
     2341 ccccgccctc aacgtagtga ccaacgacgt ggacggtgac tgtggcgggc aatacaccct
     2401 gacacccgtg atcaccggag gtacgggact ggcgcagatc ctatgggata cgggggaaac
     2461 gaccaccagc atcaccgtat cgcccggggt aaccaccacc tacgacttca cggtgacgga
     2521 cacgtgcagc ctgtcgccgg tggatggcca gatcaccgtg accattcctg tttacgagcc
     2581 gatggccgtg gaggtgagcc cgctcacctt gatcgactgc ttgagcaatg gcgatatcgc
     2641 cgtgaccaat gtgaccggtg gcgatggcga ctacgactac gcatggacct ttgaaggcgc
     2701 gc
//
LOCUS       DAOQOC010000323         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21071_length_2701_cov_3.433862, whole genome shotgun sequence.
ACCESSION   DAOQOC010000323 DAOQOC010000000
VERSION     DAOQOC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21071_length_2701_cov_3.433862"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2701
                     /locus_tag="PL106_06225"
     CDS             <1..>2701
                     /locus_tag="PL106_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32700.1"
                     /translation="IWTDPLGAVFGGSYNPALHGPGTYTYTVVGGAPCGNASAVVQVT
                     ENALPDPGTSGVFSVCSNAAAFDLPDHLGGSPDAGGVWTAPGGGPHSTVYDPAIDVPG
                     VYTYAVTGTAPCPSAAATVTVSENLAPDAGTDGTLNACSSGVPVNLFDQLIGAQSNGT
                     WELPDGTPFTGVYDPAVHGPGIYTYSVPGLPPCALDQAIVTVSESAAPYAGADATSSV
                     CANNLPTNLFPLIVGAPAGGAWTGPGGTPFTGVYDPLVHAPGTYTYTVAGVAPCPADT
                     ATVTITESDPPFAGNDASITLCDQDAPEDLFLLLGGGADGGGFWTPPAGQPPFNGIYD
                     PSTSIAGSYMYTVAGVAPCPFDVSTVTVSVVQQPYAGADTDLALCGNSAPTALIGVLT
                     GADPGGAWTAPGGGVFGGTYDPALQAPGNYTYLLPATAPCVAAQAVVTVAESAAPFAG
                     TDGAVTFCADAAPTALLPSLAGAGAGGAWTGPGGTPHSGVYDPAVDGPGVYTYTLTGT
                     PPCVSDQAVVTVTENATPWAGTDGALDICSDAATVALFGMLGGTPDAGGTWTTPGGGA
                     FGGTFDPAIDAPGPYVYTVNGIAPCGSDQSVVLVTIHAAPNAGVAGPLALCGDSPPTP
                     LFSLLVGADAGGTWTTPGGGAFNGSYDPATDGPGDFTYTVQGAPACGSDQAIVSVSED
                     PPPFAGVDADLVICGSAPAALMLPLLGGADPGGSWTAPGGGAHSGSYDPAVDGPGVYV
                     YTVSGSSACTNDMAAITVTEDVPSAATVGYAQPALCSSAGPIAVNLIGSTGGVFSAVP
                     AGLALDALSGTIDPGTSDAGAYTITYALPATGVCPAMTTTADISIAMAPDAGTDAALT
                     VCDAGAPTALFALLGGAQVGGSWTVPGGGAFTGTYDPATHAPGV"
BASE COUNT          407 a          873 c          903 g          518 t
ORIGIN      
        1 atctggaccg atccgttggg cgcggtgttc ggcggatcgt acaaccctgc gttgcacggt
       61 ccgggaacct acacgtacac ggtggtcggt ggtgcccctt gcgggaacgc gtcggcggtg
      121 gtgcaggtga ccgagaatgc gcttcccgat ccgggaacga gcggggtctt cagcgtctgc
      181 agcaacgctg ctgcgttcga cctgcccgat catctgggcg gttcgccgga cgctggtggg
      241 gtgtggaccg ctccgggtgg cgggccgcat tcgacggtgt atgatccggc gatcgatgtg
      301 cccggggtat atacgtacgc ggtaacaggt acagctccat gtccgagcgc tgcggccacc
      361 gtgaccgtgt cggagaacct ggcgcccgat gccgggacgg atggtacctt gaacgcatgc
      421 tcaagcggtg tgccggtcaa tctgttcgat caactcatcg gcgcgcagtc caatggcacg
      481 tgggaactgc cggacggtac accgttcacg ggtgtgtatg accccgcggt acacggtccg
      541 gggatctaca cgtattccgt tccggggttg cctccctgtg cgctggacca ggccattgtc
      601 accgtgtcgg aatccgcagc accctatgcc ggtgccgatg cgacatcctc ggtctgtgcg
      661 aacaatctgc ccaccaacct cttcccgttg atcgttggtg cacctgccgg cggcgcatgg
      721 accggaccgg gcggcacccc gttcacgggc gtgtatgatc cgttggtcca tgcccccggg
      781 acgtacacct ataccgttgc gggggtagca ccatgtcccg cggataccgc cacggtcacc
      841 attaccgaga gcgacccgcc cttcgccggg aacgatgcgt ccatcacact gtgcgatcag
      901 gacgctccgg aagacctgtt cctgttgttg ggtggagggg cggatggggg cggcttttgg
      961 actcccccgg cggggcagcc cccgttcaac ggcatctacg atccgtcgac cagcattgcg
     1021 ggcagttaca tgtataccgt tgcgggtgtg gccccatgtc cgttcgatgt gtccaccgtg
     1081 acggtctcgg tggtgcagca gccatacgcc ggagcggata cggatctggc cctgtgcggc
     1141 aactccgcac ccacagcgct gatcggcgtg ctcacgggag cggatcccgg aggtgcttgg
     1201 accgccccgg gaggtggtgt gttcggtgga acgtacgatc ccgcattgca ggcgcccggt
     1261 aattatacct atctgttgcc cgcgaccgcg ccatgcgtgg cggcacaggc cgtcgtgacc
     1321 gtcgccgagt cggccgcacc atttgccggc acggatggtg cggtcacctt ctgtgcggat
     1381 gcggcaccca ccgcgttgct cccgtcgctg gccggggccg gggctggagg tgcttggacc
     1441 ggtcccgggg gtacgcccca ctcaggcgtg tacgatcccg ccgtggacgg ccccggggtt
     1501 tatacgtaca cattgacagg tacccctccc tgtgtatcgg atcaagccgt ggtgaccgta
     1561 acggagaatg ccacgccttg ggccggtacc gatggcgcac ttgacatctg ctccgatgcg
     1621 gcgacggtcg cattgttcgg catgttgggc ggaacgcccg acgcgggggg gacgtggacc
     1681 acgcccggtg gcggtgcttt tgggggcacc ttcgaccccg ctatcgacgc gccaggtcct
     1741 tatgtataca ccgttaatgg tattgctccg tgcggttccg accagtccgt ggttcttgtg
     1801 accatccatg ccgcaccgaa cgccggagtt gcgggccccc tcgcgctctg cggcgattca
     1861 ccaccaacac cgctcttttc cctgctcgtc ggtgccgatg cgggaggtac ctggaccacc
     1921 cccggcggcg gtgcgttcaa cgggagctac gacccggcca ccgatggtcc cggggatttc
     1981 acgtacacgg tccaaggcgc tccggcctgt ggttcggacc aggccatcgt ctccgtgagt
     2041 gaggacccac cgcctttcgc tggagtagac gcggatctgg tcatttgtgg ctccgcaccc
     2101 gccgcgctca tgcttcccct gctcggtggc gcggatcccg gcggatcctg gaccgcaccg
     2161 ggtggtggtg cgcactccgg ttcgtacgat cctgcggtcg atggccccgg cgtgtacgtg
     2221 tacacggtga gcgggagcag cgcatgcacg aatgatatgg ccgccatcac cgttaccgag
     2281 gacgtgccat cggcagcgac cgtcggctat gcccaaccag cgctatgctc ctctgcaggc
     2341 ccgatagcgg tgaacctgat cggatcaacg ggaggggtat tttccgctgt gccggcggga
     2401 cttgcgctgg atgcgctttc agggacgatc gatcccggca ccagcgatgc cggggcgtac
     2461 acgatcacgt acgcgctgcc ggcgaccggt gtgtgcccgg ccatgaccac aacggcggac
     2521 atcagcattg ccatggctcc ggatgccggc accgacgctg cactgaccgt gtgcgatgcc
     2581 ggtgccccga ccgcactgtt cgccttgctc ggtggagcac aagttggcgg atcgtggaca
     2641 gtgcctggag gaggtgcgtt caccggcacc tatgacccgg ccacccatgc gcccggagtg
     2701 t
//
LOCUS       DAOQOC010000324         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21173_length_2694_cov_4.002653, whole genome shotgun sequence.
ACCESSION   DAOQOC010000324 DAOQOC010000000
VERSION     DAOQOC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21173_length_2694_cov_4.002653"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PL106_06230"
     CDS             <1..1114
                     /locus_tag="PL106_06230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA32701.1"
                     /translation="ERTGFVAAALSACSYLLQELTAGMINTDHNDAVFIAAVACSWWA
                     WLEHVRNGSLRWAVATGLFSACAVLTKWYVGGVVFLPWTIWLILQRFSRTALRTYLMG
                     AVALLLPVCAWLLHIITWFPAEARFEQVFKTLHFSVAMDGHSGSPWFHFEVIDTLLAP
                     LTWWIVLPACLWLLWRVQGMAHRLLICAPLLAVHLFFALAATKMICYTLVLLPIYMLA
                     IGSAMDDLIALIKHQLVRQGAFIIAFVALCAYGMNMPKVEATHSLIGNGLADPRWRQQ
                     NLMAMHDFPKLARFLSSNEKPIVFNMCRNHHLRYMFAYGTEVWAKPPDAPAVDRLRQK
                     GYSVFVIQDGADPTSFPSTTILVPDSVFSISKDVRM"
     gene            1279..2229
                     /locus_tag="PL106_06235"
     CDS             1279..2229
                     /locus_tag="PL106_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32702.1"
                     /translation="MRIPLYLLGSLLPAAVPAQILMNNFDGNNGWEELPIVIDTAANN
                     LWQVGDPDKTVFSDAYSPVNVIVTDTAAPCPPGNQSRFTVLLPSDPWGWWPEFFLHFR
                     QQVHTDEQHSGGYIEISYDTGQTWMNVFDDWMNPPNIELIDEVLGVIAPDTLSNGQLG
                     FTGEGPAGANWIWSSFCWVQTGIPLPDTLRLRFTFYTDSAATPGDGWMLDDFEYMVYM
                     AHPISEFMRKDHYFETAPNPVTDRLFVSYDTDAPTTPVRIELRDAQARLMRVLLDDEL
                     HAGRHSLMIDRDELPAQTQVCYLHATIGDRSCTERILIGP"
     gene            2344..2640
                     /locus_tag="PL106_06240"
     CDS             2344..2640
                     /locus_tag="PL106_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNA32703.1"
                     /translation="MPSPEAPFDYNTQRPRLLIPEYGRNVQRMVEHCLGIEEREARTR
                     NAHAIIQVIGRLNPQLRGGENMDRTLWDHLYIMSEFKLDVDGPYPKPTPEELDR"
BASE COUNT          487 a          913 c          752 g          542 t
ORIGIN      
        1 tgaacgcacc gggttcgtcg ctgctgcgct ttccgcgtgt tcctacctgt tgcaggaact
       61 caccgcgggc atgatcaata ccgaccacaa cgatgccgta ttcatcgccg ccgtggcctg
      121 ctcctggtgg gcctggctcg agcacgtacg caacggatcg ctccggtggg ccgtggccac
      181 agggcttttc agcgcgtgtg cggtactcac caagtggtac gttggtgggg tcgtattcct
      241 cccgtggacc atttggctca ttctacagcg cttctcgcgt acggcgctgc gcacctatct
      301 gatgggggct gtcgcactgc ttcttcctgt gtgtgcctgg ttgctgcata tcatcacatg
      361 gttccctgcg gaagcacgat tcgagcaggt gttcaaaacc ctgcacttct ccgtggcgat
      421 ggacgggcac tccggatcgc cctggttcca tttcgaggtg atcgacacct tgctggcgcc
      481 actcacctgg tggatcgtcc ttccggcgtg cttgtggctc ctatggcgtg tgcaagggat
      541 ggcgcatcgc ctgctcatct gtgcgcctct cctggccgtg catctcttct tcgcgctggc
      601 cgccaccaaa atgatctgct acacccttgt gctcctgccg atctacatgc tggccatcgg
      661 tagcgcgatg gatgacctta tcgcgctcat caagcatcag cttgttcggc aaggcgcgtt
      721 catcattgcg ttcgtggcgc tctgtgctta cggcatgaac atgcccaagg tggaggctac
      781 gcacagcctg atcggcaacg gcctggccga tccacgctgg cggcaacaga acctcatggc
      841 catgcacgac ttcccgaagc tcgcgcgttt cctgtcctcc aacgagaagc ctatcgtctt
      901 taacatgtgc cggaaccacc atttgcggta catgttcgca tacggcacgg aagtgtgggc
      961 caagccaccg gatgcccctg ccgtggaccg cctacggcag aaaggctact ccgtgttcgt
     1021 gatccaggac ggtgccgatc ccacttcctt cccttccacc actatcctgg tccctgacag
     1081 tgttttttcc atttcgaagg atgtgcgcat gtgaacacca catcacgcgg cccaatgtcg
     1141 tcatcagcta ctgcctctgc ggtgtcggag accccgacct ggtagaagcc cgcgcagcaa
     1201 gggcgaccgg cctgagcgaa gaccccccag aggcagcgcg tgcctcctgg atcacgtcct
     1261 gctggcaaac cccaccgcat gagaataccg ctatacctgc tcgggtcgct gctccctgcc
     1321 gccgtcccgg cccagatcct catgaacaac ttcgacggca acaatggctg ggaggaactt
     1381 cccatcgtga tcgacaccgc ggccaataac ctgtggcagg taggcgatcc ggacaagacc
     1441 gtcttctccg atgcgtacag cccggtgaat gtcatcgtca ccgacacggc tgcaccctgc
     1501 ccgccgggaa accagtcgcg ctttactgtc ctgctgccgt cagatccctg gggctggtgg
     1561 cccgagttct tccttcattt ccgtcagcag gtgcatacgg acgagcagca cagcggcgga
     1621 tatatcgaga tcagctatga taccggacag acctggatga acgtcttcga tgattggatg
     1681 aacccgccca acatcgaact gatcgatgaa gtcctgggtg tgatcgcgcc cgacacactc
     1741 agcaacgggc agctgggctt caccggggag ggtcccgccg gggcgaactg gatctggtcg
     1801 tccttttgct gggtgcagac cgggatccct ttgccggata ccctgcgcct gcgcttcacc
     1861 ttctacaccg actccgccgc caccccgggc gatggctgga tgttggacga cttcgagtac
     1921 atggtgtaca tggcccaccc gatcagcgag ttcatgcgca aggaccacta tttcgaaacg
     1981 gcccccaacc cggtgaccga ccgcctcttc gtgagctacg ataccgacgc gcccaccacc
     2041 cccgtgcgca ttgaattgcg cgacgcccag gcccgcctga tgcgcgtgct gctggacgac
     2101 gaattgcatg ccggccgcca cagcttgatg atcgaccggg atgaactccc cgcgcagacg
     2161 caggtgtgct acctgcatgc caccatcggc gaccggtcct gtaccgagcg catcctgatc
     2221 ggcccctgat cgtgatcccg gacgttccag ccgcctacat ttggccccgc ccggtgccaa
     2281 gcgccggtcc cgaacattgg tcacaccatc ggttggccgt tcgacccttc gatcctccga
     2341 tccatgcctt cgcccgaagc acccttcgac tacaacaccc aacggccgcg cctgctcatt
     2401 cccgaatacg gccgcaacgt gcagcgcatg gtggagcact gcctcggcat cgaggagcgc
     2461 gaggcccgca cccgcaacgc ccacgccatc atccaggtga tcggccgact gaacccgcag
     2521 ttgcgcggcg gtgagaacat ggaccgcacc ctgtgggacc acctctacat catgagcgag
     2581 ttcaagctcg atgtggatgg cccctatccc aagccgacgc ccgaggaact ggacaggtaa
     2641 cccgagcgcg tgccctaccc gaaacgcgag atccgcttcg ggcattacgg caag
//
LOCUS       DAOQOC010000325         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21192_length_2693_cov_3.225929, whole genome shotgun sequence.
ACCESSION   DAOQOC010000325 DAOQOC010000000
VERSION     DAOQOC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21192_length_2693_cov_3.225929"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..718)
                     /locus_tag="PL106_06245"
     CDS             complement(191..718)
                     /locus_tag="PL106_06245"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HNA32704.1"
                     /translation="MKLTDSTRTADDLYKAAKRWFVDAFRDAQEVIQLDDPATHMIVG
                     KGNFTYEAFIYVGSAVRHGVMRFSAEIAARNGRYRVRFYDFKHEGSSSYNTTIGRSPP
                     LDLGVIYNDQTFCTHAYNKKGEPDYEPSKHEVRVCTEEVWPQIHAFELRMLSSLKEAM
                     ENPAPASSGPGSSDW"
     gene            complement(1060..1131)
                     /locus_tag="PL106_06250"
     tRNA            complement(1060..1131)
                     /locus_tag="PL106_06250"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Glu,seq:ttc)
     gene            complement(1286..2002)
                     /locus_tag="PL106_06255"
     CDS             complement(1286..2002)
                     /locus_tag="PL106_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32705.1"
                     /translation="MKKRTAAETPKPDPQSMIVDLLRNKSGMKQDVYARTIELFRDLK
                     AALAVIADELEQRMSMADKRIQVKYTDKGEMAAELKVAGDTIIFHMHTNVFRLDQAHS
                     LWKTSYLSEDEMRGYFGVINMYNFLSDSFKYNRERDLGYLVARLFLNKDGHYFMQGKR
                     QLGFLYSDLATNTIGLPELKELLQSVILYVLDFDLLTPPYDQVNQVTVSEVNELNANM
                     QISTGKRLGFRFQADVEDVS"
     gene            complement(2006..>2693)
                     /locus_tag="PL106_06260"
     CDS             complement(2006..>2693)
                     /locus_tag="PL106_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNA32706.1"
                     /translation="QEHVWATSWGVSTRLMGALIMTHSDDHGLVLPPKLAPIQVAIVP
                     IAKNAEQLEALRGYVKPMIEGLRARGITVKFDDDDTKKPGWKFAEYEFKGYPVRIAVG
                     PRDMENGTVEVARRDTLEKQVMQVADLVAKVEHLLEAIQSNLFQKALAMRENSTRAVD
                     SYDEFKVAIEEGGFILAHWDGTAETEARVKEETKATIRCIPLQGERTPGRCMVTGQPS
                     AQRVIFARAY"
BASE COUNT          515 a          804 c          789 g          585 t
ORIGIN      
        1 ccgccgtatc accgcggaag cggagaccct gcgagggtgc accaccctgg taacacagca
       61 cccgaccttc accttcacat gaccgaactt cgggaaagca tacacgccca aggtgcgccc
      121 acaacttcca agcgaaatgc gggagatccc agtatatcgg agtagcccca atgcgcggat
      181 cgcgtatgtc tcaccaatcg gaagaaccgg gtccgctgct cgctggggcc gggttctcca
      241 ttgcctcctt taaactggag agcatacgga gttcaaaggc gtggatctgc ggccacactt
      301 cttcggtgca aaccctcacc tcatgttttg aaggttcata gtcgggttcg cccttcttgt
      361 tgtacgcatg cgtgcagaag gtctggtcgt tatagatcac gccgaggtcc agcggtggac
      421 tacggccgat tgtcgtgttg taggaggatg agccctcatg cttgaagtca taaaagcgga
      481 cgcggtatcg cccattccgg gccgcaatct ccgccgaaaa tctcatcacc ccatgccgaa
      541 ccgcactccc cacgtagatg aatgcctcgt acgtgaaatt gccttttcca acaatcatgt
      601 gtgttgcagg gtcgtcgagt tggatcactt cctgggcatc acggaatgcg tccacaaacc
      661 aacgtttggc cgccttgtat aggtcgtcgg ccgtccgtgt actgtccgtc agtttcacaa
      721 cctcgattcg ttcgtacgga gcggctgcat acgtgggcgt aggttcttgg gaaaaggccg
      781 agatgagggt cacggccaag gctgcactta gtaggattcg catgttccaa atgtatctgg
      841 gcgcagatca taagtggcac acgggaaacc gggcacaacc aacgaccaag ccgacaaccc
      901 aaaagcgaaa acgctggaat cccttctatt gctaaggttt tggcgttccg gatgcagccc
      961 gtaggaaatg accgactgat ccgggcggat cggcccgtgg ggctgttccc ctacgggcca
     1021 caagcagaac gccccggcgt gtgccgaggc gtaactgggt agcccgtagg ggaatcgaac
     1081 ccctgtttca tccgtgaaag ggacgcgtcc taacccctag acgaacgggc cacgatgaca
     1141 aacccttctt gcgaaggggg cgcaaatgta atcggatgcg ggtctggtcc aaatctgacc
     1201 gtcctgccat cccataacag gtgggctggg ccgaaggcgg acaacaacgg gcggtccaac
     1261 ggcaggtcat ggaccgaagg aacggtcagg acacgtcctc cacatcggcc tggaaccgga
     1321 agcccaggcg tttgccggta ctgatctgca tgttggcgtt caactcgttc acctcgctta
     1381 ccgtcacctg gttcacctgg tcgtacgggg gagtgaggag gtcgaagtcc agcacgtaga
     1441 gaatgacgga ctggagcagc tccttcagct cgggcaggcc gatggtgttg gtggccaggt
     1501 cgctgtacag gaagccgagt tggcgcttgc cctgcatgaa atagtggccg tccttgttca
     1561 ggaagaggcg cgccaccaga taccccaggt cccgctcccg gttgtatttg aaggagtcgc
     1621 tgaggaagtt gtacatgttg atcaccccga agtacccgcg catttcatcc tcgctcaggt
     1681 agctggtctt ccacaggctg tgtgcctgat cgagccggaa gacgttcgtg tgcatgtgga
     1741 agatgatggt gtcgcccgcc accttcagtt cagccgccat ctcgcccttg tcggtgtact
     1801 tcacttgaat gcgcttgtcg gccatggaca tgcgctgttc caattcatcg gcgatcacgg
     1861 ccagggccgc cttcaggtcg cggaaaagct cgatggtccg cgcatataca tcctgcttca
     1921 tgccgctttt gttgcggagc aggtccacga tcatcgactg gggatcgggc ttgggtgttt
     1981 cagcggctgt tctcttcttc atggctcagt acgccctggc gaagatgaca cgttgtgcgc
     2041 tcggttgtcc ggtcaccatg caccggccgg gcgtgcgttc gccctgtaaa ggaatgcatc
     2101 ggatggtggc cttggtttct tccttcactc gggcctcggt ctccgccgtg ccgtcccagt
     2161 gggccaggat gaagccccct tcttcaatgg ccaccttgaa ttcgtcgtag ctgtccacgg
     2221 cccgggtgct gttctcgcgc atggccaatg ccttttggaa gaggttcgac tggatggcct
     2281 ccagcaggtg ctccactttg gcaacgaggt ccgccacctg catcacctgc ttttccaacg
     2341 tatcgcgccg ggccacctcc accgttccgt tctccatgtc gcgcgggccc acggcgatac
     2401 gtacgggata gcccttgaac tcgtactcgg cgaatttcca gcccggcttc ttggtgtcgt
     2461 cgtcgtcgaa cttcaccgtg atgccgcgtg cgcgcaagcc ctcgatcatg ggcttcacgt
     2521 agccgcgcaa tgcctccaac tgttcggcat tcttggcgat gggcacaatg gccacctgta
     2581 tcggggccag cttgggtggc agtaccaggc cgtggtcgtc gctgtgggtc atgatcaggg
     2641 cgcccatcag gcgggtgctc acaccccagc tggtggccca cacgtgctcc tgt
//
LOCUS       DAOQOC010000326         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21299_length_2687_cov_3.006079, whole genome shotgun sequence.
ACCESSION   DAOQOC010000326 DAOQOC010000000
VERSION     DAOQOC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21299_length_2687_cov_3.006079"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="queA"
                     /locus_tag="PL106_06265"
     CDS             <1..849
                     /gene="queA"
                     /locus_tag="PL106_06265"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNA32707.1"
                     /translation="MWGNKEKTGAKIEVFLLRELNRDSLLWDVVVDPARKIRIGNKLY
                     FGKDDELVAEVIDNTTSRGRTLRFLFDGTYEEFKSAITAMGETPLPHYIKRDIEPSDA
                     ERYQTIYAQHEGAVAAPTAGLHFSRELMKRMELKGIHFAGVTLHIGLGTFRPVEVEDL
                     TKHKMDSEQVLITQDVCDMVNKSKERKKNVCCVGTTTMRAIESSVSTDDRMKSYNGWT
                     NKFIFPPYDFSIANRMVTNFHMPESTLLMMVCAFGGYDNVINAYKVALKEKYRFFSYG
                     DAMLII"
     gene            934..1596
                     /locus_tag="PL106_06270"
     CDS             934..1596
                     /locus_tag="PL106_06270"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNA32708.1"
                     /translation="MHRSTIIVAGGIGKRLGGPVPKQFQTVKGRPLLMWTIEAFHRFD
                     PAMAIIVVLPPTHFDIWKALCMGHRFFVHHDVVAGGEQRWHSVKAGLEKVEHDGLVAV
                     HDGVRPLVSTDLIARCFAAADEKAAAIPVVPVVPSIRETTADGSRALDRSRLVAVQTP
                     QCFHADLLRKAFEQTYESTFTDEATLVERMGVKVALVEGESNNIKVTTAADMRLVEML
                     LG"
     gene            1614..2669
                     /gene="rlmN"
                     /locus_tag="PL106_06275"
     CDS             1614..2669
                     /gene="rlmN"
                     /locus_tag="PL106_06275"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNA32709.1"
                     /translation="MVAPTDIRSLSLDEVKNLVAEIGEKPYRAKQLWEWLWKKKARSF
                     DDMSDLPKVFRAALAERTVLRPLVLVEEQLSQDGTVKCAFRTWDGHVVEGVLIPTPTR
                     LTACISSQIGCSLSCSFCATGKLKRVRNLDSQEIYDQVVLIDQLAIKYHKEGLTNIVY
                     MGMGEPLLNYANTLRSAERITAEDGLGMGARRLTVSTAGIAKMIRKLADDGARFNLAL
                     SLHAANDAKRDRIMPINEQNTLTELKDALRYYTRITRKEVTFEYILLRDFNDSLADAQ
                     ELVKYASDVHAKVNLIEYNPIDSGGFGRTDAKDAEAFQRYLDRKGIIARIRRSRGRDI
                     DAACGQLANKNEAVAKA"
BASE COUNT          573 a          833 c          827 g          454 t
ORIGIN      
        1 atgtggggca acaaggagaa gaccggggct aagatcgagg tcttcctgct gcgcgagctc
       61 aaccgcgaca gcctcctgtg ggacgtggtg gtggaccccg cacgcaagat ccggatcggc
      121 aacaagctgt acttcggcaa ggacgacgag ctggtggccg aggtgatcga caacacgacc
      181 agccggggcc gcacgttgcg gttcctgttc gacggcacgt acgaggagtt caagtcggcc
      241 atcaccgcca tgggcgaaac gccgctgccg cactacatca agcgcgacat cgagcccagc
      301 gatgccgaac gctaccagac catctatgcc cagcacgaag gggccgtggc cgcacccacc
      361 gcgggcctgc acttcagccg cgagttgatg aagcgcatgg agctcaaggg catccatttc
      421 gccggtgtta ccctgcacat cggcctgggt accttccgtc cggtggaagt ggaggacctc
      481 accaagcaca agatggacag cgagcaggtg ctcatcaccc aggacgtgtg cgacatggtg
      541 aacaagagca aggagcgcaa gaagaacgtc tgctgcgtgg gcaccaccac catgcgcgcc
      601 atcgagagca gcgtgagcac cgacgatcgc atgaaatcgt acaacggctg gacgaacaag
      661 ttcatcttcc cgccgtacga cttcagcatc gccaaccgga tggtgaccaa cttccacatg
      721 cccgagagca ccctgctcat gatggtgtgc gccttcggcg gatacgacaa cgtgatcaac
      781 gcgtacaagg tggccctcaa ggagaagtac cgattcttca gttacggcga cgcgatgttg
      841 atcatctgag aaaagcgtga atgggcgtaa gggtgcgagg gttcagactc tcgcaccctt
      901 tctccttccc actcgtcaac gacctttgga tccatgcatc gctccaccat catcgtagca
      961 ggcggtatcg gcaagcgcct cggcgggccc gtgcccaagc agttccagac cgtcaagggc
     1021 cgcccgctgc tcatgtggac catcgaggcc ttccatcgct tcgatcccgc catggcgatc
     1081 atcgttgtgc tgccacccac ccacttcgac atctggaagg ccctgtgcat ggggcatcgc
     1141 ttcttcgtac accatgatgt ggtggccggt ggcgagcaac gctggcacag tgtgaaagcc
     1201 ggtttggaga aggtggagca cgatgggctt gtggccgtcc acgacggggt gcgtccgttg
     1261 gtgagtaccg acctcattgc ccgttgtttc gccgctgcgg atgaaaaggc cgccgccatc
     1321 cccgtggtgc ccgtggtacc cagtatccgg gagaccaccg cggacggcag ccgcgcactg
     1381 gaccggtcgc ggttggtggc ggtacagacg ccccaatgct tccatgccga cctgctgcgc
     1441 aaggcgttcg aacagaccta cgaaagcacc ttcaccgatg aggccacact ggtcgagcgt
     1501 atgggcgtga aggtggcgct ggtggaaggg gagtcgaaca acatcaaggt gaccacagcg
     1561 gcggacatgc ggttggtgga gatgctgctc ggctgagccc gtacattcgg aacatggtag
     1621 cacccaccga tatccgctct ctctcgctcg acgaggtgaa gaacctcgta gcggagatcg
     1681 gcgaaaagcc ttaccgagcg aagcagctct gggagtggtt gtggaagaaa aaggcccgct
     1741 cgttcgatga catgagcgac cttcccaagg tattccgcgc cgcgctggcc gaacgcaccg
     1801 tgcttcggcc gctggtattg gtggaagagc agctcagcca ggatggcacc gtgaagtgcg
     1861 ccttccgcac atgggacggc cacgtggtgg aaggcgtact gatccccacg cccactcgtc
     1921 tcacggcctg catcagcagc cagatcgggt gcagcctcag ttgttcgttc tgtgccaccg
     1981 gcaagctcaa gcgcgtccgc aacctcgatt cgcaggagat ctacgaccag gtggtgctga
     2041 tcgatcaact cgcgatcaag taccataagg aaggactgac gaacatcgtg tacatgggca
     2101 tgggcgagcc gctgctcaac tatgccaaca ccttgcgcag tgcggagcgc atcaccgccg
     2161 aggacggact gggcatgggc gcccgtcgcc tcaccgtgag caccgccggc atcgccaaga
     2221 tgatccgcaa gctggccgac gatggtgccc gcttcaacct ggccctcagc ctgcatgccg
     2281 ccaacgatgc caagcgcgac cgcatcatgc ccatcaacga gcagaacaca ctcaccgaac
     2341 tgaaggacgc cctgcgctat tacacccgca tcacccgcaa ggaggtcacc ttcgagtaca
     2401 tcctgctccg ggacttcaac gactcgctgg ccgatgccca ggaactggtg aaatacgcca
     2461 gcgatgtaca cgccaaggtg aacctgatcg agtacaatcc catcgactcg ggaggtttcg
     2521 ggcgcacgga cgccaaggat gccgaggcct tccagcggta cctggaccgg aagggcatca
     2581 tcgcgcggat ccgccgtagc cgggggcgcg atatcgatgc ggcctgcggg cagctggcca
     2641 acaagaacga agcggtggcg aaggcgtgat cgtgtgctgg aattgca
//
LOCUS       DAOQOC010000327         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21366_length_2682_cov_3.071565, whole genome shotgun sequence.
ACCESSION   DAOQOC010000327 DAOQOC010000000
VERSION     DAOQOC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21366_length_2682_cov_3.071565"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..506
                     /locus_tag="PL106_06280"
     CDS             174..506
                     /locus_tag="PL106_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32710.1"
                     /translation="MDIEAARTTTLAHFVAEEYDHFGRIAYRIPPKKAGGKPGRTFLT
                     LWPDTGYAVLMLDADLQAGLLDHPSGAFGPHPSKWGAKGATIMLLEKVNVKIFHEAVG
                     IAHAHAKR"
     gene            complement(507..1259)
                     /locus_tag="PL106_06285"
     CDS             complement(507..1259)
                     /locus_tag="PL106_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA32711.1"
                     /translation="MKVSVITVCYNAAIHIGDTLRSISGQDHDDIEHIVIDGGSTDGT
                     LDVIHTYRSGIAQLVSERDRGVYDAMNKGLRMATGDVVAFVNAGDMIEGAATVSAMAS
                     EFAKGDADAIYGDALMVDPDDISKVKRFWKGGTYARERFRAGWMPPHLGTYIRRDVYE
                     RLGHFREDLKVSADYELMFRFMYKHRIRVRYVPKVLVRFRLGGVSNRSLAHIWRANVE
                     VYKAWGLNGEQVSPLIVVAKPLRKIAQLFRRG"
     gene            complement(1256..1822)
                     /locus_tag="PL106_06290"
     CDS             complement(1256..1822)
                     /locus_tag="PL106_06290"
                     /inference="COORDINATES: protein motif:HMM:NF007797.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HNA32712.1"
                     /translation="MTNERVHVDLSRFDNADFPKGAGPVKLALWYFTNALFFLNPLFP
                     FRRVKPALLRLFGAKVGRGVVIHPGVNIKYPWKLIIGNHCWVGQRAWLDNIDLLTIGD
                     HVVISQGAVIIQGSHDYKKVDYPTMSGPVTLESGSWVGAGAMVLMGVRLGSHSVLAAG
                     SVASADLMPYMIYRGNPARPVRERTISA"
     gene            complement(1819..>2682)
                     /locus_tag="PL106_06295"
     CDS             complement(1819..>2682)
                     /locus_tag="PL106_06295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA32713.1"
                     /translation="DAVYINGMWSPWFSAMPLWLLRGSSVKRIVAVRGMLARGMMKHG
                     AMKKRLFLAAMKLAGCYKNVVFQATNTEEMEDVKRWISADAVVHLVPNLGRKAVGGAS
                     SSRVKRPGELQLVSVARIAVEKNTLFAIECLAHVRGRVSFDLYGPIYDEAYWAKCREA
                     IARLPEGITVTHKGTIAAEDVPALFAKYHALFMPSLGENFGHSMVEALATGLPLLISD
                     RTPWRGLAALNAGWDLPLEDPDGFRDVLNDLVMCDQAGFDPLSKGAFALGERYLNDPA
                     PVRATLELLLR"
BASE COUNT          549 a          845 c          789 g          499 t
ORIGIN      
        1 gtctcgacct aagtaaacag tttctagctc ggaaaggtga tcagggtgag cagtgcgatg
       61 atgatgtcca gtatctccat gggaaggccg atcgatcggg gtgcaaagaa gcatcagaag
      121 cggctccgca caccggaaag ccaggccggt gaccggttcc tattttgggc cgcatggaca
      181 tcgaagccgc gcgcaccacg acccttgcgc acttcgtggc ggaggagtac gaccatttcg
      241 ggaggatcgc ctaccggatc ccgccgaaga aggcaggcgg caaaccgggc cgcaccttcc
      301 tgaccttgtg gccagatacc ggatacgccg tattgatgtt ggacgcggac ctgcaggccg
      361 ggcttctgga ccatccatcg ggcgcattcg gaccgcaccc ttccaaatgg ggagcgaaag
      421 gcgccacgat catgctgctg gaaaaggtga acgtcaagat cttccacgag gcggtgggga
      481 tcgcgcatgc gcacgcgaag cgttgatcag cctctgcgga acaactgggc gatcttgcgg
      541 agaggcttgg ccacaacgat cagcgggctc acctgttcac cattcaggcc ccacgccttg
      601 tacacctcca cgttggcacg ccagatatgc gccagggagc ggttgctcac cccgcccaaa
      661 cggaagcgca ccaacacctt gggcacgtag cgcacgcgga tccgatgctt gtacatgaag
      721 cggaacatga gttcataatc ggcgctcacc ttcaggtcct cgcggaagtg gcccaggcgc
      781 tcgtacacat ccctgcggat gtaagtgccg aggtgcggtg gcatccagcc ggcccggaac
      841 cgctcgcgcg cataggtccc tcctttccaa aagcgtttca ccttcgagat gtcgtccggg
      901 tccaccatga gcgcatcccc gtagatcgcg tcggcatcgc ccttggcgaa ttcggaggcc
      961 atggccgaaa cggtggctgc cccttcgatc atgtcgcccg cgttcacaaa agccaccaca
     1021 tcgccggtag ccatgcgaag acctttgttc atcgcgtcgt acacgccccg atcacgttcg
     1081 ctcacgagct gcgcgatccc gctccgatag gtatggatca catcgagggt gccatcggta
     1141 ctgccaccgt cgatcacgat atgctcaata tcatcgtggt cctgcccgga gatgctgcgc
     1201 aacgtatcgc cgatatggat ggcggcgttg tagcagacgg tgatgacgga gaccttcacg
     1261 cggagatggt ccgttcgcgc acggggcgcg ccgggtttcc ccggtagatc atgtagggca
     1321 tcagatcggc actggccacg ctgcctgccg ccagcacact gtgcgagccg agccgcacgc
     1381 ccatgagcac catggccccc gcccccaccc agctaccgct ttccaaggtg acaggtccgc
     1441 tcatggtggg ataatccacc ttcttgtagt cgtggctgcc ctggatgatc accgcccctt
     1501 gcgaaatgac cacatgatcc ccgatggtga gcaggtcgat attgtccagc catgcgcgct
     1561 ggcccaccca gcagtggttc ccgatgatca acttccacgg gtatttgata ttgacgcccg
     1621 ggtggatgac cacgccccga cccactttgg ccccgaaaag ccggagcagt gcgggcttca
     1681 cgcgacggaa cgggaaaagc ggattgagga agaagagcgc gttggtgaag taccacaggg
     1741 ccagtttgac cggtcccgct cctttcggga aatcggcatt gtcgaatcgg ctcaggtcga
     1801 cgtgtacgcg ttcgttggtc atcgcaacag aagttccagc gtggctctca ccggcgcggg
     1861 atcgttcaaa tatcgttcgc cgagcgcgaa ggcccctttc gataagggat cgaacccggc
     1921 ttggtcgcac atgaccaaat cgttcagtac atcccggaaa ccgtcggggt cctccaaggg
     1981 caggtcccat ccggcgttca gtgcggctaa tccacgccac ggcgtccgat cgctgatgag
     2041 gagcggaagc ccggtggcca gcgcttccac catggaatgg ccgaaattct cgcccaggct
     2101 gggcatgaat agcgcatggt acttcgcgaa cagggcgggg acatcttccg cggcgatcgt
     2161 tcccttatgc gtcacggtga tcccttcagg gagccgggcg atggcttcac ggcacttcgc
     2221 ccaataggcc tcgtcgtaga tcgggccata gagatcaaag gagacccggc cacgcacatg
     2281 cgcaaggcat tcgatggcga aaagtgtgtt cttctcaacc gcgatccgtg ccacactcac
     2341 cagttgcagt tcaccgggcc gcttcacacg ggatgacgag gcgccaccaa cggccttgcg
     2401 gcccagattg gggaccagat gtaccacagc gtccgccgat atccaacgct tcacgtcctc
     2461 catctcctcg gtgttcgtgg cttggaacac gacattcttg tagcatcccg cgagtttcat
     2521 ggccgccagg aagagccgct tcttcatggc accatgcttc atcattccac gagccagcat
     2581 gccccgtacg gccacgatgc gtttgacgga ggaaccgcgc aggagccata agggcatggc
     2641 actgaaccac ggcgaccaca tgccattgat gtacaccgca tc
//
LOCUS       DAOQOC010000328         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21385_length_2681_cov_3.258568, whole genome shotgun sequence.
ACCESSION   DAOQOC010000328 DAOQOC010000000
VERSION     DAOQOC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21385_length_2681_cov_3.258568"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..905)
                     /locus_tag="PL106_06300"
     CDS             complement(369..905)
                     /locus_tag="PL106_06300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNA32714.1"
                     /translation="MEPILIHPLHTAFGELLLGSFNNELCLCDWRYRRMRTTIDARIQ
                     CGVQAEYTEGASPVIEQAKAQLNAYFAGERTTFDMPLRMVGTDFQQRVWQTLLTVPYG
                     TTLSYAALTAKVAEPTAIRAVASANGANALSIMVPCHRIIGSNGEMTGYAGGVRVKRR
                     LLELEGVTVTSLQPTLFG"
     gene            1245..2108
                     /locus_tag="PL106_06305"
     CDS             1245..2108
                     /locus_tag="PL106_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32715.1"
                     /translation="MARLLPVFMLMLPITAHAQSWCPPGAEWTYEYIDQMFNSGVHRI
                     EHTGDTLIDGHQAQRLTHWVHYGGGWGQPALQTAHWNDLFTYEENGVVFLKSDWFQGF
                     DTVMWFSAAPGDRWDAPGIEDWAFVVLDTTTILLDGLPLRQLIVQMEPQDLFPVDTLR
                     ERIGFQWFFINPVESMLIDGNYAGLRCYQDDELFHAMPNVSDCGYTMSVQAGAQEPRA
                     SIFPNPGTDHFNVALPPGAHVIRLCDAIGRELLNLRALGPNVRVDTAIFLAGIYFIGV
                     DDQRPERWVKE"
     gene            complement(2193..>2681)
                     /locus_tag="PL106_06310"
     CDS             complement(2193..>2681)
                     /locus_tag="PL106_06310"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNA32716.1"
                     /translation="QLPVMARSAFAQPERELQYAAVDLLVKHARKLTPEHLPLLEELI
                     TTKSWWDTVDALAVHVVGEVLKRHPAHIASWNERWIESDDLWLNRTAILFQNRWKQET
                     DQALLFANIDRHALHEDFFIRKAIGWALRELGKTNPGAVLDFVRSRKLSPLSEREALR
                     GL"
BASE COUNT          508 a          853 c          780 g          540 t
ORIGIN      
        1 tcttcctgtg cgtgcgcaag ggcctggtac cacgcccctg gttcacggcc accgtcgtgc
       61 tggtgctgcc catggtgggg ctcctgggct actggggcct gctctggcct tgatggcccg
      121 tgcgcagcaa cacggactta ccggaccaca tagtgatccg catcggaacc ttgatgtcga
      181 tcatccgcct gagcggcttg gatcgatccc tgttcgcata ggcatacagc ctacgttcgt
      241 ggtcccagta gaccagcttc ccagttgccg cttccaaggt gtgcgacgca gcccggctct
      301 gcgggcgaag gcatatcccg ggcggaaagc cctcatgacc ggacagcata tccaacctgc
      361 ggggttccct atccaaaaag tgtgggctgc agcgaagtca ccgtcacccc ctccaactcc
      421 aacaacctcc ttttcaccct caccccgccg gcataacccg tcatctcccc gttgctgccg
      481 atgatgcggt ggcagggcac catgatcgag agcgcgttcg cgccgttcgc ggaagccacc
      541 gcgcggatcg ccgtgggctc cgcaaccttc gcggtcagtg cggcatagct caacgtggtg
      601 ccgtagggaa ccgtcagcaa ggtctgccat acgcgttgct ggaagtcggt gcccaccatg
      661 cgcaagggca tatcgaaagt ggtgcgttca cccgcgaagt acgcattcaa ttgcgccttt
      721 gcttgctcga tcaccggcga agcaccttcg gtatattcgg cctgcacgcc gcactggatg
      781 cgcgcatcga tggtggtacg catgcgccga tagcgccaat cgcacaggca cagttcgttg
      841 ttgaacgaac ccagcaacaa ctcgccgaac gcggtgtgta atggatggat gaggatcggt
      901 tccatggcga gaaagtaatc gcaaggtctt cacaagcccc tcagcgcttc ctgctcgttc
      961 agcgcactga gcttccgcga gcgtacgaag tccatcaccg cacgcggatc ttcccgctgg
     1021 ggcgggacaa gttgtgcttg ggaaggtagg gggcggcgac ggcgtgtgac atgtcgcgaa
     1081 aatcccggtg agcgcatgaa agcgcatgtg cctacgggcc acccgttggg ctgcgagcca
     1141 ccccggcccc gagcctgggc ctgagccgac attttccgtg caacaacacc cgtggggcag
     1201 cgatcaacaa gatcttcgcg tccaacctgt acattcacga gtccatggca cgtttgcttc
     1261 cagtattcat gctgatgctg cccatcacgg cccacgccca gagctggtgc ccgccgggtg
     1321 ccgaatggac ctacgaatac atcgaccaga tgttcaattc gggcgtccac cgcatcgaac
     1381 acacgggtga caccttgatc gatggtcacc aggcccaacg gctcacgcat tgggtccact
     1441 atggcggtgg atgggggcag cccgcattgc agaccgcgca ttggaacgac cttttcacgt
     1501 atgaggagaa tggcgtggtc ttcctgaaaa gtgactggtt ccagggcttc gataccgtga
     1561 tgtggttcag cgccgcgccc ggagatcgat gggacgctcc aggcatcgag gactgggcgt
     1621 tcgttgtcct cgataccacc acgatcctgc tggatggcct tcccttgcgt caactgatcg
     1681 ttcaaatgga gcctcaagac ctgtttccgg tggacaccct gcgcgaacgt atcgggttcc
     1741 agtggttctt catcaacccg gtcgaatcca tgctgatcga tgggaattat gcaggcctgc
     1801 gctgctatca ggacgatgag cttttccatg cgatgccgaa tgtgtcggat tgcgggtaca
     1861 ccatgtcggt ccaagccggt gcacaggaac cacgtgcctc catcttcccc aaccccggca
     1921 ccgatcactt caacgtggcc ctgcctccgg gagcacatgt cattcgcctg tgcgatgcta
     1981 tcggtaggga gttgctgaac ctccgtgcgc tgggaccgaa cgtgcgcgtg gataccgcaa
     2041 tatttcttgc cgggatttat ttcatcggtg tggacgacca gcgaccagag cgctgggtga
     2101 aggagtagag tggcaaccac ggcggacctc cgctgtgcaa acgatgatcg cacacccatt
     2161 ggtcgtgctg tccccgctgg accatgcagc acctacaagc cccgcaacgc ctcccgctca
     2221 ctcagcgggc tcagcttccg ggaacgcaca aaatccagca ccgccccggg attggtcttg
     2281 cccagctccc gcaacgccca gccgatggcc ttgcggatga agaagtcctc gtgcaacgca
     2341 tgccggtcga tgttcgcgaa gagcagcgct tggtccgttt cctgcttcca ccggttctgg
     2401 aagaggatcg ccgtgcgatt caaccacagg tcatcgcttt cgatccagcg ttcattccaa
     2461 ctggcgatgt gggcggggtg ccgcttgagc acttcgccta ccacatgcac cgccaacgca
     2521 tccaccgtgt cccaccagct tttggtggtg atcaattcct ccagcaaggg caaatgctcc
     2581 ggcgtgagct tccgcgcgtg cttcaccagc aggtccacgg cggcgtattg caattcgcgt
     2641 tcgggctgcg cgaaggccga tcgtgccatg accggcagct g
//
LOCUS       DAOQOC010000329         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21566_length_2669_cov_2.966335, whole genome shotgun sequence.
ACCESSION   DAOQOC010000329 DAOQOC010000000
VERSION     DAOQOC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21566_length_2669_cov_2.966335"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2193
                     /locus_tag="PL106_06315"
     CDS             64..2193
                     /locus_tag="PL106_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNA32717.1"
                     /translation="MKVFDDKHIKNVVLLGSHGCGKTTLAETMLFEAGLIPRRGRVED
                     NNTVSDHHALEHERGSSVYSTVLHTEWRNYKINIIDTPGLDDLIGETIPALRVADTCL
                     LLLNAHHGVEVGTDLVWDHIAQYDRPVIIGVNQVDHPNADFDATVDQAKEHFGNAVTV
                     MQYPVESGEGFHRIIDLLKMTMYVFKDEGGKPEKQPIPANEKEKADAQHKELVEKAAE
                     NDEALMEHYFDKGELDEDEMRQGLKQGMMKRTCFPVFCLSALRNMGTGRLMGFIDNVA
                     PGAIEMPAEELVDGGTLPCRADGPAVLFAFKTVIEPRSGHITLFKVMSGEVKEGADLV
                     NDNTGSAERLNQLFILDGKERRPVERLVAGDIGGTIKLKDTSSAHTLHEPGKAIKLRP
                     IAFPEPRLRQVIKAADQKLEEKLHAALLEIQKEDPTIVLTYQRETGQQIVGGQGDLHL
                     NLLKWKLNHHYKVDATYHAPRIPYRETIRRGAEASYRHKKQTGGSGQFGEVHLRIEPW
                     YEGMPAPQGVSVRDIQEHDLPTGGKLVFCNCIVGGVIDNKFMPSILKGVMEKMERGPL
                     TGSPARDIRVVVYDGKMHPVDSNDISFRIAGLQAFREAFGNADPQLMEPIQEVEVRVP
                     ADLMGDVMTDLQGRRSIVMGMDTQGRYQIIKVHTPLAELDRYSTTLRSLTQGRGTYTE
                     RFLAYQPVPAELQHKLVSSHKEDEVVV"
     gene            complement(2261..>2669)
                     /locus_tag="PL106_06320"
     CDS             complement(2261..>2669)
                     /locus_tag="PL106_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32718.1"
                     /translation="QLDWYFDEWINTVRSLDYGVAAITGRGDSTFITLERKGEMLMPV
                     DVAVMGTDGRTTCYQVPLSLMLGARPGSPSGAPCTALRPWLWTAPRYTFGIPVPMDAV
                     RAVILDPEERLADKDRSNDSVELEPGTRGVVRP"
BASE COUNT          589 a          833 c          844 g          403 t
ORIGIN      
        1 cgttgctcta aacccgcccg ccgatgatgt gaacatgctg aagcgggcac aaccctgcca
       61 accatgaaag tgttcgatga caagcacatc aagaacgtcg tattgctggg ctcccacgga
      121 tgcggcaaga ccacgctggc cgaaaccatg ctcttcgaag cgggtcttat cccacgccgc
      181 ggccgtgtcg aggacaacaa taccgtgagc gaccatcatg ctctggagca tgagcgcgga
      241 agcagcgtgt acagcacggt gctgcacacc gagtggcgca actacaagat caacatcatc
      301 gacacgccgg ggctggacga cctcatcggc gaaaccatac cggccttgcg ggtggccgac
      361 acctgcttgc tgctgctgaa cgcgcatcat ggcgtggagg tgggcaccga cctggtgtgg
      421 gaccacatcg cgcaatacga ccgcccggtg atcatcggcg tgaaccaggt cgaccacccc
      481 aacgcggact tcgacgccac ggtggaccag gcgaaggagc acttcggcaa cgccgtcacc
      541 gtgatgcaat accccgtgga gagcggcgag ggtttccacc gcatcatcga cctgttgaag
      601 atgaccatgt acgtcttcaa ggacgagggc ggaaagccgg agaagcagcc cataccggcc
      661 aacgagaagg aaaaagcgga tgcgcagcac aaggaactgg tggagaaggc cgcggagaac
      721 gacgaggccc tgatggaaca ctacttcgac aagggcgaac tggacgagga cgagatgcgg
      781 cagggcctca aacaggggat gatgaagcgg acatgcttcc ccgtcttttg cctgagcgca
      841 ttgcgcaaca tgggcaccgg ccgcctcatg ggcttcatcg acaatgtggc gcccggggcc
      901 atcgagatgc ccgccgagga actggtggac ggcggcaccc tcccttgccg ggctgacggt
      961 ccggcggtgc tgttcgcttt caagaccgtg atcgaaccgc gcagcggtca catcacgctc
     1021 ttcaaggtga tgagcggcga agtgaaggaa ggcgccgacc tggtgaacga caataccggt
     1081 tcggcggagc gcctgaacca attattcata ctcgacggaa aggaacggcg acccgttgag
     1141 cggcttgtgg ccggcgatat cggcgggacc atcaagttga aggacacgag cagcgcgcat
     1201 accctgcacg agccgggcaa ggccatcaaa ttgcgcccta tcgccttccc cgaaccgcgc
     1261 ctgcgccagg tgatcaaggc cgccgaccag aagttggagg aaaagttgca cgccgcgttg
     1321 ctggagatcc aaaaggagga ccccaccatc gtactgacgt accagcgcga gaccggccag
     1381 cagatcgtgg gcggacaggg cgacctgcac ctgaacctgc tgaaatggaa gctgaaccac
     1441 cactacaagg tggacgccac ctatcatgcg ccgcgcatcc cctaccgcga aaccatacgg
     1501 cgtggggccg aggcgagcta ccggcacaag aagcagaccg gcggcagcgg ccagttcggc
     1561 gaggtgcatc tgcggatcga accgtggtac gaaggcatgc ccgcaccgca gggagtgagt
     1621 gtgcgcgaca tacaggaaca cgacctgccc acggggggaa aactcgtttt ctgcaactgc
     1681 atcgtgggcg gtgtcatcga caacaagttc atgcccagca tcctcaaggg cgtgatggag
     1741 aagatggaac gcggccccct caccgggtcg cccgcgcgcg acatccgcgt ggtggtgtac
     1801 gatggcaaga tgcatcccgt ggacagcaac gacatcagct tccggatcgc gggtctgcag
     1861 gccttccggg aggcgttcgg caatgccgat ccgcaattga tggagcccat tcaagaagtg
     1921 gaggtgcgcg tacccgccga cctgatgggc gatgtgatga ccgacttgca agggcgccgc
     1981 agcatcgtga tgggcatgga cacccaaggc cgctaccaga tcatcaaggt acacacgccg
     2041 ctggccgaac tggaccggta cagcaccacg ttgcgcagcc tcacccaagg gcgcggcacc
     2101 tacaccgagc gcttcctggc ctaccagccc gtgcccgccg aattgcagca caagctggtg
     2161 agttcgcaca aggaggacga ggtggtggtg tagatcctgc caacgagcga ccgcccgctg
     2221 ttgcgctccg atagggggat acgacagcgg gcggcaacgc tcagggccgc accacgcccc
     2281 gcgttcccgg ttccagctcc acgctgtcgt tgctccggtc cttgtccgcc aaacgctcct
     2341 cgggatccag gatcacggca cgcaccgcat ccatggggac cggaatgccg aaggtgtagc
     2401 ggggcgctgt ccacaaccac gggcgaagag ccgtgcatgg ggccccggag ggggatcccg
     2461 ggcgggcacc gagcatcagg gagagcggca cctggtagca tgtggtacgg ccatccgttc
     2521 ccatcacggc cacatccacc ggcatgagca tctccccttt gcgttccaag gtgatgaatg
     2581 tgctgtcgcc acggccggtg atggccgcca ccccgtagtc cagtgatcgc acggtgttga
     2641 tccactcatc aaagtaccaa tccagctgc
//
LOCUS       DAOQOC010000330         2665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21628_length_2665_cov_3.620307, whole genome shotgun sequence.
ACCESSION   DAOQOC010000330 DAOQOC010000000
VERSION     DAOQOC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21628_length_2665_cov_3.620307"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PL106_06325"
     CDS             <1..291
                     /locus_tag="PL106_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis protein WbpP"
                     /protein_id="HNA32719.1"
                     /translation="RCALAVGEAASGQVFNIAYGDRCDLLALTDRLRSHLARLDPNIA
                     QVRVEHAPGRAGDVRDSLADISRAREQLGFMPAFDLEDGLTRAVPWYAENWR"
     gene            complement(331..1341)
                     /locus_tag="PL106_06330"
     CDS             complement(331..1341)
                     /locus_tag="PL106_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32720.1"
                     /translation="MTARSIATAILLASTATLLAQRNLEVGISTGVTQYYGDLGNWNG
                     QVQWNSTRPALNITFRDFFNNPKRYVTRSLTMECRLSWFRIGYNEVQPTGGESGIGLH
                     NFKRGLNFRNDLFGASAHMVLNAYREPFTPLFKQRFFMYFHLGIGMYYGRPKADLFRG
                     DPDLSNRYYYWEDGSIRDTPRGSAEGTVIEQDGKYETDLYSWTIEGRGKGSSSTIQER
                     TSPWNIGIPMGCGIRYMVTRKMSIGAEFSYYMFLTDMLDAVSERYLTYDEIGQVYADS
                     TQQYLARYISDPTGWGTDGTQGFRTSRRGNPGLPDNFSYFSFEVSYKLDRRPGRRNYM
                     SF"
     gene            complement(1406..2083)
                     /locus_tag="PL106_06335"
     CDS             complement(1406..2083)
                     /locus_tag="PL106_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNA32721.1"
                     /translation="MDQQRLVERYERVRSSVPARVKLIAVSKTRSVEEIHALYALGQR
                     AFGENYPQELRAKAPLLPPDIEWHFIGHLQRSNVKHVAGLAQLIHGVDSERLMDELSK
                     RARAQGRTQDILLQVHIAQEDTKHGFTPDELRALMEGSDMAAKWPGLRIRGLMGMATN
                     TPDRARISGEFAGLAHLFNDIVASRVFPDRSFTELSMGMTGDMDLAIAAGSTMVRVGT
                     AIFGERG"
     gene            complement(2334..>2665)
                     /locus_tag="PL106_06340"
     CDS             complement(2334..>2665)
                     /locus_tag="PL106_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNA32722.1"
                     /translation="FSLRLPEPLSTNPGDRLDAGLLSDLVLGPVLGIQDLRTDPHVRF
                     VPGTAGPMELERMVQQGEAAVAFHLCPVSFEELKSVADAGGCMPPKSTWIEPKLWSGL
                     TVYPLED"
BASE COUNT          490 a          828 c          819 g          528 t
ORIGIN      
        1 cgttgcgcac tcgccgtcgg ggaggccgca tccggtcagg tattcaacat cgcctacggt
       61 gatcggtgtg atctgctggc actcacggat cgccttcgtt cgcatctcgc acgcctggac
      121 ccgaacatcg cccaggtgcg tgtggaacat gcaccgggcc gcgcgggcga cgtacgcgat
      181 tcgctggccg acatctccag ggcgcgtgaa caactcggct tcatgcccgc tttcgacctg
      241 gaggacgggc tgacccgcgc ggtaccgtgg tacgcggaga attggaggtg agcgggccgc
      301 ccgcgatcgc gccccggtgg tcctgcccgc tcagaagctc atgtaattgc gccgacccgg
      361 ccgccgatcc agtttgtagc tcacctcaaa ggagaagtaa ctgaagttgt ccggaagccc
      421 ggggttgcca cgtcggctcg tacggaaccc ctgggtccca tcagtacccc aacccgtggg
      481 atcgctgatg taccgtgcca ggtactgttg tgtgctgtcg gcgtacacct gtccgatctc
      541 atcgtacgtc aggtaccgtt cgctcaccgc gtccagcatg tccgtcagga acatgtaata
      601 actgaactcc gcgccgatgc tcattttccg tgtgaccatg tagcggatgc cacagcccat
      661 cggaatgccg atattccacg ggcttgtgcg ctcctggatg gtactgctgg agcccttgcc
      721 gcgcccttcg atggtccagg agtacaggtc ggtctcgtac ttgccgtcct gctcgatcac
      781 cgtgccttcc gccgagcccc gtggggtgtc cctgatgctg ccgtcctccc agtaataata
      841 cctgttgctc aggtccgggt cgccgcggaa caggtccgcc ttggggcggc cgtaatacat
      901 gccgatgccc aggtggaaat acatgaaaaa acgctgtttg aacagcggcg tgaagggctc
      961 ccgataggcg ttgagcacca tgtgcgcgct ggcaccgaag aggtcgttcc ggaagttgag
     1021 cccacgcttg aagttgtgca gtccgatccc ggattctcca cccgtcggct gcacctcgtt
     1081 gtagccgatg cggaaccagc tcaggcggca ttccatggtg agcgatcgcg tcacgtaccg
     1141 cttcgggttg ttgaagaagt cgcggaatgt gatgttcaac gcgggccgcg tgctgttcca
     1201 ctgtacctgg ccgttccagt tgcccaggtc gccgtagtac tgggtgacac cagtggagat
     1261 accgacctcc aaattgcgct gggccaggag cgtggccgtg gacgcgagga ggatggctgt
     1321 ggcgatggac cgggcggtca tggttccggc gcttgggcta gccaagatac gaagaggaga
     1381 cgtcctcccg gccaaatggt tgccgtcagc cccgttcccc gaaaatggcc gtgcccaccc
     1441 gtaccatcgt actgcccgca gcgatcgcca ggtccatgtc gcccgtcatg cccatgctca
     1501 attcggtgaa ggaacggtcc gggaataccc tggatgcgac gatatcgttg aacagatgtg
     1561 ccagtccggc gaattcaccg gaaatacggg cccgatcagg ggtgttcgtg gccatgccca
     1621 tcagaccgcg gatgcgcagc ccaggccact tcgccgccat gtccgagccc tccatgagtg
     1681 cgcgcaactc atccggagtg aagccgtgtt tggtgtcctc ctgggcgata tgcacctgaa
     1741 gcaggatatc ctgcgttcgg ccctgtgccc gtgcacgctt gctcaattcg tccatcaggc
     1801 gctcgctgtc cacaccatgg atcaattgcg ccaggcctgc cacgtgcttc acgttcgatc
     1861 gctgcagatg gccgatgaaa tgccattcga tgtccggcgg gagcaagggt gcctttgccc
     1921 tcaactcctg ggggtagttc tcgccaaagg cccgctgccc cagcgcataa agcgcgtgga
     1981 tctcctccac actacgcgtc ttgctcaccg cgatcaactt gacccttgcc ggaacggacg
     2041 acctcacacg ttcatagcgt tcgacgagcc gctgctggtc cattgatgcg aagatgcgta
     2101 ggacttacgg accagtaccg catcccggta cggaatggtc caaagttgcg cagacgtttc
     2161 cgtaagcggg atgaagcatt tttcgcagga cgaatcggga aggtcgtagc gcagtttggg
     2221 ctacggaacg atctccccgg tgaagccgcg cggacaacga gcaagcagga cctgttccat
     2281 cagggcacaa gggaccacgc tgtattgcga aatggtattc ccgggtgccg gtgtcaatcc
     2341 tccaacgggt acacggtgag cccgctccag agcttgggct cgatccaggt gctttttggg
     2401 ggcatacagc caccggcatc ggcaacggac ttcaattcct cgaagctcac ggggcacaaa
     2461 tggaaggcta ccgcggcttc gccctgctgc accatgcgct ccaattccat ggggcctgcg
     2521 gttcccggaa cgaaacgcac gtgcgggtcc gttcgcagat cctgtatgcc gagcacgggt
     2581 ccgagcacga ggtcgctgag gagcccggcg tccaagcggt cgcccgggtt ggttgacaag
     2641 ggttcgggaa gccgcaggga gaacc
//
LOCUS       DAOQOC010000331         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21638_length_2664_cov_5.509007, whole genome shotgun sequence.
ACCESSION   DAOQOC010000331 DAOQOC010000000
VERSION     DAOQOC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21638_length_2664_cov_5.509007"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PL106_06345"
     CDS             <1..207
                     /locus_tag="PL106_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32723.1"
                     /translation="YTGGSNDRDPILLQVGSTTPNATAPAYLPADINLDGTVKYTGGS
                     NDRDRVLITVGSTTPNATRTEQLP"
     gene            286..789
                     /locus_tag="PL106_06350"
     CDS             286..789
                     /locus_tag="PL106_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA32724.1"
                     /translation="MTAFSLRPFRHNDLLALVRHANDPTVAAFLTDLFPHPYTEENGR
                     KFLEEAMKPVPLRRCIEVDDECIGAIGLHPKQDLWRYNMELGYWLARAYRGKGIITSA
                     IRQMVRIGFATFPDVTRIYASPFGSNIASQQALEKAGFTLEAKLIGTLVKNGKVEDEW
                     IYGIRRD"
     gene            complement(839..>2664)
                     /locus_tag="PL106_06355"
     CDS             complement(839..>2664)
                     /locus_tag="PL106_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32725.1"
                     /translation="GNFAVKNNLLIGANADGGTGLITVDGSGAQTYSVAAGSPRTCRV
                     EVDKPSGSFTPAMGTTDFSVQAFSLVAGDFTAPSGDLNVGGTWTTSQTLFSHSGGTYA
                     HNNGTLLVNPYCNVGPMLTWTLDVLPTTVLYDVTINANQAWTMPQCLIPAADSVHVDG
                     ILLLSDGRVNGGTLDAHGAVQLLPGYDGGNARLLFHGSGAQTFDLSGASGNYNGDIGI
                     DKPAGNVQLLSDLVMDAGGQDLQFAHGDLLTTANEILIMGDNVACSGASDSSFVDGPM
                     RKVGNDAFTFPIGGEGIYYAPIRISAPGNVNDAFTAEYFHDDPQDIPYDVSAVGAGLD
                     HVSRCEYWDLERTAGSSNVRVTLSWAARSCGVNLLSDLCVARWNGSQWDSHGNGGTTG
                     TMAAGTVITHSALTPTAYGPFTLGSTTSNNPLPVELLSFDAWENDPVVDLKWVTATEH
                     NNAYFVVQRTVDGLAFEDVDTVQGAGNSVAEISYTAVDPSPHRGISYYRLRQVDLDGT
                     SKYSELVAVDLTGVADGSWVLYPNPSVDGPVWLTATGDRWDGPGVLTVVDGAGRVVEK
                     RTVVFTDGPVELMKGLHPEAGMYSVHVVMNGVEHNKRLIIQ"
BASE COUNT          530 a          892 c          751 g          491 t
ORIGIN      
        1 tataccggtg gaagcaatga tcgcgacccg atcctgctgc aggtgggcag caccaccccc
       61 aacgccaccg cacccgccta cctgcccgcc gacatcaatc tggacggcac ggtgaaatat
      121 accggcggaa gcaacgaccg cgatcgcgtc ctgatcacgg tggggagcac cacccccaac
      181 gccacccgaa cggaacaact cccctgagga cgggaccacc cttgtccgcc ccgatgcgaa
      241 cggagccccg gccgaatggc cggggctccg ttcctttggc acggtatgac cgcattctcc
      301 ctccgaccat tccgccacaa cgacctgttg gcactggtga ggcacgccaa cgaccccacc
      361 gtggcagcgt tcctcacgga cctgttcccg cacccgtaca ccgaagagaa tggccgcaaa
      421 ttcctggaag aggccatgaa gcccgtcccg ttgcgccgat gcatcgaagt ggatgatgag
      481 tgtatcggcg ccattgggct gcacccgaag caggatcttt ggaggtacaa catggaactc
      541 ggctactggc tggcccgtgc ataccgtgga aaagggataa taacctcggc catccgccag
      601 atggtccgga tcggcttcgc cacattcccg gatgtcacgc gcatatacgc ctctcccttc
      661 ggcagcaata tcgcgtcgca acaggcgctg gaaaaggccg gcttcacgct ggaagcgaag
      721 ctgatcggca cactggtgaa gaacgggaag gtggaggatg agtggatcta cggcatacgg
      781 cgggattgag gatcacaacg ttcccgggct gacgtcccac ctgcatccag gacctccttc
      841 actggatgat cagccgcttg ttgtgctcca caccgttcat caccacatgg acggaataca
      901 tgcccgcttc cgggtgcaac cccttcatca actcgaccgg tccatccgtg aagacgacgg
      961 tccgcttctc caccacccga cctgccccgt cgacaacggt gagcaccccc ggcccatccc
     1021 aacggtcgcc cgtggccgtg agccacacgg ggccgtcaac ggaggggttc gggtacagca
     1081 cccatgaacc atccgcaaca cccgtcagat cgaccgccac cagctcgctg tacttgctcg
     1141 tcccgtccag gtccacctgg cgcaaacgat agtaggaaat gccccgatgt ggcgaaggat
     1201 cgaccgccgt atagctgatc tccgcaacgc tgttgcccgc accctgaacg gtgtccacat
     1261 cttcgaacgc gagtccatcc acggtacgtt gcaccacgaa atacgcattg ttgtgctccg
     1321 ttgcggtgac ccacttgaga tcaacaaccg ggtcgttctc ccaagcatcg aaactcaaca
     1381 gttccaccgg cagcgggttg ttcgaggtgg tggaaccgag ggtgaacggt ccataggccg
     1441 ttggtgtcag tgcgctgtgg gtgatcacgg ttcctgcggc catcgtgccg gtggtacccc
     1501 cgttcccatg gctgtcccat tggctgccgt tccatcgggc cacacacaga tcgctcaaaa
     1561 ggttcacgcc acaactacgt gcggcccaac tcagggtcac gcgcacgttg gatgagcctg
     1621 cggtccgctc caggtcccag tactcgcacc ggctcacgtg gtccaaccct gcgcccacgg
     1681 ccgacacgtc atacggaatg tcctgcggat catcatggaa gtactccgcc gtgaaggcat
     1741 cgttcacgtt cccgggggcg gagatcctta tcggtgcgta gtagatccct tcgccgccta
     1801 tcgggaatgt gaacgcatcg ttgcccacct tgcgcatggg gccgtcaacg aagctgctat
     1861 ccgatgcccc ggagcaggcc acgttatcac ccataatgag tatttcattg gccgtggtga
     1921 gcagatcacc atgtgcgaac tgcaagtcct gtccaccagc gtccataacg aggtcggaca
     1981 gcaattgcac attcccggcg ggtttgtcga tcccgatgtc accgttatag ttgccggatg
     2041 cacccgaaag gtcgaaggtc tgcgcaccgg acccgtggaa gagcagccgt gcgttgcccc
     2101 catcataacc tggcagcagc tggaccgcac catgtgcgtc gagcgttcca ccattcacgc
     2161 gcccatcact caacagcagg atcccatcca catggaccga atccgccgca gggatcaggc
     2221 attgcggcat cgtccatgcc tggttcgcgt tgatggtcac atcgtacagc accgtcgtgg
     2281 gcaatacatc cagcgtccag gtgagcatcg ggcccacatt gcaatacggg ttcaccaaca
     2341 gggtgccgtt gttgtgcgcg tacgtgcctc cgctgtggct gaagagtgtc tggctcgtgg
     2401 tccaggtccc gcccacgttc aggtcgccgc tgggcgccgt gaagtccccg gccaccaggc
     2461 tgaaggcctg taccgaaaag tccgtagtgc ccatggccgg ggtgaagctg cccgagggct
     2521 tgtccacttc gaccctgcac gtgcgtgggg atccggccgc cacgctgtac gtctgtgcgc
     2581 cgctgccatc caccgtgatc agacccgtac cgccatccgc gttcgctccg atcagcaggt
     2641 tgttcttcac ggcgaagttg ccaa
//
LOCUS       DAOQOC010000332         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21642_length_2664_cov_4.463396, whole genome shotgun sequence.
ACCESSION   DAOQOC010000332 DAOQOC010000000
VERSION     DAOQOC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21642_length_2664_cov_4.463396"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..>2664)
                     /locus_tag="PL106_06360"
     CDS             complement(220..>2664)
                     /locus_tag="PL106_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HNA32726.1"
                     /translation="FRFMECPVMHPDLQAQLPMIRTYAGFGIDEPGASVRFDRTPHGF
                     HAMVLTTRREAWFIDPYAFGNTTDLICYRKVDFRKSLPPDFHYCAFDELTVPENIERR
                     TQELLRSLDGERTGDCQFRTYRLALACTGEYANFHGGTVPLAAAAMVTTLNRVNQMFE
                     RDATLTLQLIPDNNDLIYLNSNTDPYTNNDGGTMLDENMSTCNSVIGEDNYDIGHVFS
                     TGGGGVAYLASVCSDYKAGGVTGSSSPVGDPFDIDYVAHEMGHQFNGNHTQNNDCNRE
                     PDAAVEVGSGITILGYAGVCSPDVAPHSDAMYGGYTLGEMHSFITGSGHTCDAVTSPV
                     NAPPTANAGVDRTIPRSTPFILSGTGTDADVGNVLTYSWEQMNSTPATQPPIATSTAG
                     PCFRPMLPDTSNKRFLPNLPAVIANTTPTWEVLASVGRTYNFRLTVRDNAPGGGCNAQ
                     DDMKVTVNATAGPFLVTAPNTALTWAGGSTQTVTWNVAGTASAPVNCTNVDILLSTDG
                     GLTWPIILASSTPNDGSHTVILPDLATTTARVIVKASANIFYDMSNANFTITSAAQAQ
                     LNLRLWLEGPFDGNTQLMHDSLRAKGLLPLTEPYSAMGFANGGGGGGEITTAGMLATT
                     GNTAIVDWVRVELRSQADPGTLVATRHALLRRNGEVVSASGNSALTFGVATGNYYVAV
                     IHRNHLGTMSLNAVNMTNGTPTLDLRSTATATYGTGARKIVGNTAMLWAGNAVRSSPS
                     PEQLKYTGAGNDRDPILIAVGSNTPNNTLPNVYDARDTNMDGRVKYTGGENDRDHILT
                     NIGSTTPNNVRTQQTP"
BASE COUNT          422 a          764 c          931 g          547 t
ORIGIN      
        1 gaatggacgt attacctggg tgatcgacaa ggagggggtg gtccgctatc tggaggtcgc
       61 gaaggaactg ggcagcgaac ccgattacga ggcagcgctc aacgaggcga agggcctgct
      121 gtagcccggg tcacatcgga tcgaacaact ttctgccggg cacaacgacc gacgggtacc
      181 cgtgggtgaa caccgcttgg gatccctcct gcgcatgcat cagggcgtct gctgcgtgcg
      241 cacgttgttg ggcgtggtgc tgccgatgtt ggtcaggatg tggtcccggt cgttctcgcc
      301 gccggtgtat ttcacccggc catccatgtt ggtatcacgc gcatcataca cgttgggcaa
      361 ggtgttgttc ggcgtgttgc ttcccacggc gatcaggatc gggtcgcgat cgttcccggc
      421 accggtatat ttgagttgct caggcgatgg tgaggaccgt acggcgttcc cggcccagag
      481 catcgcggtg ttgccaacga tcttccgcgc accggtgccg taggtggcgg tcgccgtgct
      541 gcgaaggtcc agtgtgggtg tgccgttggt catgttcacg gcattcagcg acatggtgcc
      601 caggtggttg cggtggatca ccgccacgta gtagttccct gtggccacgc cgaaggtgag
      661 cgcggagttg ccgctggcgc tgaccacttc gccgttgcgg cgcaacagcg catgacgcgt
      721 ggcgacaagc gtgcccggat cagcttgtga gcggagctcc acgcggaccc agtccacgat
      781 ggcggtgttc ccggtggtgg cgagcatacc cgccgtggtg atctccccac cgccgccgcc
      841 accgttagcg aagcccatcg ctgagtacgg ttccgtcagc ggcagcaggc ccttggcccg
      901 caaactgtcg tgcatgagct gggtgttgcc atcgaacgga ccttccagcc agaggcgcag
      961 gttcagttgt gcttgtgcgg ccgaggtgat cgtgaagttg gcattgctca tgtcgtagaa
     1021 gatgttggca ctggccttca cgatcacgcg tgccgtggtg gtggccaggt cgggcaggat
     1081 cacggtgtgg ctgccatcat tcggtgtgct gctggccagg atgatcggcc acgtgaggcc
     1141 gccgtccgtg ctgagcagga tgtccacgtt cgtacaattc accggcgccg atgcggtgcc
     1201 agctacgttc caggtcaccg tctgtgtgct gccgccggcc caggtgaggg ccgtgttggg
     1261 agcggtcacc aggaaagggc cggccgttgc gttcacggtc accttcatgt cgtcctgcgc
     1321 gttgcagcca ccaccgggag cgttgtcgcg cacggtgagg cggaaattgt acgtgcgccc
     1381 caccgaggcc agcacctccc atgtgggcgt ggtgttggcg atgacggcgg gcaggttcgg
     1441 caggaaacgt ttgttgctcg tgtcgggcag catggggcgg aaacacggcc cggccgtgct
     1501 ggtggcgatc ggaggctgcg tggccggggt gctgttcatc tgttcccagc tgtaagtgag
     1561 cacattgccg acatccgcgt cggtgcctgt gccgctgagg atgaacgggg tgctgcgcgg
     1621 gatcgtacgg tccactccgg cgttggcggt gggcggggcg ttcacgggtg aggttaccgc
     1681 atcgcaggta tgtccggaac ccgtgatgaa gctgtgcatc tcgcccagcg tgtaaccacc
     1741 gtacatcgca tcgctgtgcg gggccacgtc gggcgagcaa acaccggcgt atcccaggat
     1801 cgtgatcccg ctgcccactt ccaccgcggc gtccggttcg cggttgcagt cgttgttctg
     1861 ggtatgattc ccgttgaact ggtggcccat ctcatgcgcg acgtagtcga tgtcgaacgg
     1921 gtcgcccacc ggggaactcg aacccgtgac gccgcccgct ttgtagtccg aacacacgct
     1981 ggcgaggtag gccacaccgc caccaccggt gctgaacaca tggccgatgt cgtagttgtc
     2041 ctcgccgatc acgctgttgc acgtgctcat gttctcgtcc agcatggtgc cgccatcgtt
     2101 gttggtgtac gggtcggtgt tgctgttgag gtagatgagg tcgttgttgt cggggatcag
     2161 ttgcagggtg agcgtggcgt cccgctcgaa catctggttc acgcggttca acgtggtcac
     2221 catggcggct gcggccaaag gcaccgtgcc cccgtggaag ttcgcgtatt ctccggtgca
     2281 ggccagggca aggcggtatg tgcggaactg gcagtcgccg gtgcgctcgc catcgagtga
     2341 tcggagcaac tcctgtgtgc gccgctcgat gttctccggt acggtcagtt cgtcgaaggc
     2401 gcagtaatgg aagtcaggag gaaggctttt gcggaagtcg acttttcggt agcagatcag
     2461 gtcggtcgtg ttaccgaatg cgtacgggtc gatgaaccag gcctcccgcc gggtcgtgag
     2521 caccatggca tggaaaccgt gcggcgtgcg atcgaagcgg accgaggcgc ccggctcgtc
     2581 gataccgaaa ccggcgtagg tgcggatcat gggcaattgc gcctgcagat cggggtgcat
     2641 cacggggcat tccatgaagc ggaa
//
LOCUS       DAOQOC010000333         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21688_length_2661_cov_3.294321, whole genome shotgun sequence.
ACCESSION   DAOQOC010000333 DAOQOC010000000
VERSION     DAOQOC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21688_length_2661_cov_3.294321"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2661)
                     /locus_tag="PL106_06365"
     CDS             complement(<1..>2661)
                     /locus_tag="PL106_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32727.1"
                     /translation="FIEARHAGAVPIPYVAPYTGTQPITGITLDPATGLVNFTLNMAG
                     NWVVIVKVNEYDSAGNLIGSIMRDMQFIAYPCNNAPPDPASGTVGGVSGTAVQTAPYA
                     IRMCESGTACFDMVITDPNPANVLQATSNIAANLPGATFSYTGTNPIQCHVCWNGGQG
                     TAGYYPFIVNVNDGACPIVGIQTYVYTVEVIEGVFIDVTTSPESCAGQCDGSATASVV
                     SGTGPFTYAWDNGATSNPLTACAGTYVVTVTDANGCVTTPAQAVIDGSTPPTADGGPD
                     VSVCYGQWPIAMTGTATNSAATGWSGGTGLWSGTGSTMYYTPSVAEVTAGGADVVFTA
                     TGNGGCPDATDQVHIILSNSYISAAITPTNITCNGAATGSATYAPDLPGNTYVWNAVP
                     PQTAATASGLMAGNYSVTVTDAQGCTATMSTTITQPAALVITGVNATDETCAGQGNGT
                     LVASASGGTGGQQNYVYNWSNGATGQTITVGAGTYSVTVTDANGCTSAAAQGTVNSAA
                     LPNAADAGNDAMVCMNDFPIALSGSVQNATGGQWSGGTGSFAGQTPSTFYTPSTAEVA
                     AGYVDLVLTTTGNTGCPPASDQVHIIISNSFLNAAVSTTPILCNGGSTGTAAFSPAQP
                     VYGYQWSPSGQNGPVATGLSGGTHTVTVTDQLGCDTVMSVSLIDPPTLTVTAAQSTGP
                     LCANGTNGSITLSVTGGTAPYTYQWSTNAGGQTTATANNLGAGAYQVTVTDANGCIAQ
                     ASATLTAPAPIMLTAQVPDTVCVNTPIQLTAQVSGGTGATTIYWGGIGYGPSMQYSFP
                     TSQIVSVSATDAVGCTSPILTFPVNVLDLNAATLVMSNDTTVCPGGTAQVSAAVNNYP
                     GAINIQWTELVTTGTGPFNVPVSNTQTLHVL"
BASE COUNT          394 a          809 c          915 g          543 t
ORIGIN      
        1 agtacgtgca gggtctgtgt attgctcacc ggtacgttga atgggccggt gcccgtggtg
       61 accagttcgg tccattggat gttgatggcg cccgggtagt tgttcaccgc tgcgctcacc
      121 tgggccgtac cgcccgggca caccgtggtg tcgttggaca tcaccagtgt ggcggcgttc
      181 aggtcgagca cattcaccgg gaaggtcagg atcgggctgg tgcaccccac ggcatcggtg
      241 gccgatacgc tcacgatctg cgaggtgggg aaggagtatt gcatggacgg gccgtagccg
      301 atccctcccc aatagatcgt ggtggctccc gtgcccccgc tcacctgtgc ggtcagttgt
      361 atgggcgtgt tcacgcagac cgtgtccggg acctgggccg tcagcatgat cggtgcgggt
      421 gcggtgagcg tggcgcttgc ttgtgcgatg cagccgttgg cgtccgtaac ggtcacctga
      481 tatgcgcccg cgccgaggtt gttcgccgtg gccgtggtct gcccacccgc attggtgctc
      541 cattgatagg tatacggggc cgtgcctccg gtcaccgaca aggtgatgct gccattggtg
      601 ccgttggcac agagcggtcc ggtggactgt gccgctgtca ccgtcagggt gggcgggtcg
      661 atcagcgata cgctcatcac ggtatcacat ccgagctggt ccgtcaccgt cacggtatgc
      721 gtaccgcccg aaagaccggt ggccacggga ccgttctgtc cgctggggga ccattggtag
      781 ccgtagacag gttgggcagg gctgaaggcc gccgtaccgg tgctgccgcc gttgcacagg
      841 atgggcgttg tgctcacggc cgcgttcagg aagctgttgg agatgatgat gtgtacctgg
      901 tcgctggcgg gcgggcatcc tgtgttgccg gtggtggtga gcacaaggtc cacgtagccc
      961 gcggccacct cggcggtcga tggggtgtag aatgtgctcg gcgtctggcc cgcgaaggag
     1021 cccgtgcctc cgctccactg gccaccggtg gcgttctgca cgctacccga gagggcgatg
     1081 gggaagtcgt tcatgcagac catggcgtca ttgcccgcat cggcggcgtt gggcagggcg
     1141 gccgagttca ccgtgccttg tgccgccgca ctggtacagc cgttcgcatc cgtcacggtc
     1201 acggagtagg tgcccgcgcc tacggtgatc gtctggcccg tggccccgtt gctccagttg
     1261 tacacgtagt tctgctggcc ccccgtgccg ccgctggcgc tcgccaccag tgtgccgttg
     1321 ccctggcccg cgcacgtttc atcggtcgcg ttcacgcccg tgatcacgag tgcagcgggt
     1381 tgggtgatcg tggtgctcat ggtggcggtg cagccttggg cgtccgtcac cgttacgctg
     1441 taattgcccg ccatcagtcc ggatgccgtg gccgcggtct gcggtgggac agcgttccag
     1501 acataggtgt tgcccggcag atcgggcgcg taggtcgcag agcctgtcgc ggcaccattg
     1561 caggtgatgt tggtgggtgt gatcgctgcg ctgatgtagc tgttcgacaa gatgatatgt
     1621 acctgatcgg tggcatccgg acaaccgccg ttccccgtgg ccgtgaagac cacatcggca
     1681 ccgccagcgg tcacttcagc cacgctgggc gtgtagtaca tggtgctgcc cgtgccgctc
     1741 cacaagccgg ttccgccgct ccagccggtc gctgcggaat tggtcgccgt accggtcatg
     1801 gcgatcggcc actggccgta acacacgctc acgtccggtc ctccatcggc ggtgggaggt
     1861 gtgctgccat cgatgaccgc ctgggccggg gtggtgacac atccgttcgc gtcggtcacg
     1921 gtgacgacat aggtcccggc gcaggcggtc agtgggttgc tcgtggcgcc attgtcccaa
     1981 gcgtaggtga acggcccggt tccggatacc acactggcgg tagcgcttcc gtcgcattgg
     2041 cccgcgcagc tctccgggct ggtggtcacg tcgatgaaca ccccttcgat cacctccacc
     2101 gtgtacacat aggtctggat gcccacgatc ggacaggccc cgtcgttcac gttcacgatg
     2161 aagggatagt acccggccgt gccttggccg ccattccagc acacatggca ctggatggga
     2221 ttggtgcccg tgtagctgaa ggtagcaccg ggcaggttgg ccgcgatgtt gctggtggcc
     2281 tgcaacacgt tggccgggtt cgggtcggtg atgaccatgt cgaaacatgc cgtaccgctt
     2341 tcgcacatcc ggatcgcgta gggcgcggtc tgcacggcgg tacccgatac acctccaacg
     2401 gtacccgatg cggggtcggg aggggcgttg ttgcacgggt aagcgatgaa ctgcatgtcg
     2461 cgcatgatcg aaccgatcag gttgcccgca ctgtcgtact cgttcacctt cacgatcacc
     2521 acccaattgc ccgccatgtt caacgtgaag ttcacaaggc ccgtggccgg gtccagggtg
     2581 atgcctgtga tcggctgcgt gccggtgtac ggggccacat acggaatggg cacggcgccc
     2641 gcatggcgag cttcgatgaa c
//
LOCUS       DAOQOC010000334         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21705_length_2660_cov_3.709405, whole genome shotgun sequence.
ACCESSION   DAOQOC010000334 DAOQOC010000000
VERSION     DAOQOC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21705_length_2660_cov_3.709405"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            495..>2660
                     /locus_tag="PL106_06370"
     CDS             495..>2660
                     /locus_tag="PL106_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32728.1"
                     /translation="MATTNLPVSVFKLATVRAPRKAIIDEAPARRIVYTLNSAFYTTL
                     KTNRAGANPRPTMIASATTFKTTNALYIADLLALKALFPGFDRIDDYLRTAQRNAVKA
                     DLKALVEGATVLNQTAAAYVSAGGYSALKSRVWDNVFALTVLGGEAVLRTEEVRVIRL
                     LNVIEKIAANDTLLDTGAGIFSAYLSNVLLPADVFPMPDVLLPDEPTTEPIPADPVLA
                     AAVAKLANVQQAYTALAKAYADQVLAYRAKVHPSEKPDPTAPPQAPLIGHDPLALEKT
                     RYISLDTKVRTTLEGYGVTSDFVYVPTLKERLVEDMRELGRTILATSTRDRVVRVGSS
                     LIQVSNECAQLPPVSPCSPFNADNLVQWPGGAGHIRPLGIADLRVVQSQLYKYELGEV
                     AHVENILKGESKKRTFRNLQRTETSTTTDSETTRETEKETQTTERFELHQEANNVVQQ
                     DQQSQIGVTASYGFPAGGSASLSASTASSTSEMEATTQATTYAKDVMERALNRVIERT
                     RTTRTVTTIIEHEDTTEHGFTNVQVEDAPAPENIAGVYRWLDKVYYNKVVNYGKRLLF
                     EFIIPEPAAFHIFSAMAKPASDQVHELPPPFDIASFNEIRPDTYDALVIKYGAVGVKP
                     PPTPIVSVNEVFSEEPTPTGSSKFNAYHANVQIPAGYKAMHARVSSIWSHGQDVNGNW
                     HYVWFAVDTQPYIWRYQSGEDVIPLSQQTGTIAINSISYT"
BASE COUNT          583 a          850 c          736 g          491 t
ORIGIN      
        1 atgccctggc cgatgcggtt ggggccgcta ccgagcacga tgatcttctt gcggtcgctg
       61 cgcacgctct cgttctcctc ctcaaaagtg ctgtagtaat acggcgtctt cgcggggaac
      121 tcaccggcgc aggtgtccac gatgtacatt gcttgggcgt gcccccgtcc caaatgcggc
      181 cggggtcgcg tgcttcgctt tagttgcctt agcccggcct gcatccacgg cgctccgggg
      241 tcacctcctt cgtcggtgcc tcctcgctgc tcgtggctgc ttggccgcgc cttcggcaag
      301 ctgccgctac gcccgctcac gcgatgccga cgtctgcccg cgatcaccgt gacgagcagt
      361 tttcaacacc ggcaatgatc aactcagtgg atcgttcacg gtgaaaacac acatgcccct
      421 acctgtgaaa aatcgccggc tcacaggtcg caacttgtgc tgagatttga cctgtcttcc
      481 attccccctt caccatggcc accaccaacc tcccagtcag cgtcttcaag ctcgccactg
      541 tacgtgcgcc gcgcaaggcc attattgatg aggcaccagc gcgccggatc gtgtacacgc
      601 tgaacagcgc gttctacact acgctgaaga cgaaccgggc tggcgcgaac ccaaggccga
      661 cgatgatcgc atcagccacc actttcaaaa ccacgaacgc gctttacatc gcggacctgt
      721 tggcgctgaa ggcactcttc ccaggtttcg accgcattga cgattacctg cgcaccgcgc
      781 aaaggaatgc ggtgaaagcg gacctgaagg ccctggtcga aggagcgact gtattgaacc
      841 agaccgctgc cgcctatgtg agtgcgggcg gctacagcgc cttgaaatcg cgcgtatggg
      901 acaatgtctt cgcattgacc gtacttggtg gggaagcagt gcttcgcacc gaagaagtgc
      961 gtgtgatccg cttgctcaat gtcatcgaga agatcgcggc gaacgataca ctgcttgata
     1021 caggtgcggg cattttcagt gcgtacctct ccaatgtgct gctacctgcg gacgtattcc
     1081 ccatgcccga tgtcctcctt cccgatgaac caactacgga gcccatacca gccgaccctg
     1141 ttttggccgc agccgttgcc aaactggcca atgtacaaca ggcctatacc gcacttgcca
     1201 aagcgtatgc ggaccaagta ctggcctatc gcgccaaagt gcatcctagc gaaaagccag
     1261 atccaaccgc gcctccacaa gcgcccctga tcggtcacga cccgcttgct ttggagaaga
     1321 cgcgctacat cagtttggat acgaaggtgc ggaccacact ggagggatac ggtgtcacca
     1381 gcgatttcgt gtatgtacct acgctgaagg aaaggcttgt cgaggatatg cgcgagctcg
     1441 gccgtaccat cctcgcgact tccacccgcg accgtgtggt gcgtgtgggc agcagcctca
     1501 ttcaagtgag caacgaatgc gcgcagctgc ctccggtctc accatgcagc ccgttcaatg
     1561 cggacaacct ggtacaatgg cccggcgggg ctggccacat ccgtcctttg ggcattgccg
     1621 acctgcgcgt ggtgcaaagc caactgtaca aatacgaatt gggcgaggtg gcgcatgtgg
     1681 agaacatcct caagggcgaa tccaagaaac gcaccttccg gaacctccaa cgcaccgaga
     1741 ccagcaccac caccgacagc gagaccaccc gcgaaaccga gaaggaaacc cagaccaccg
     1801 aacggttcga attgcaccaa gaggcgaaca atgtggtgca gcaggatcag cagagccaga
     1861 tcggggtcac cgcgtcctac ggtttcccgg ccggaggcag cgcctcgcta agtgccagca
     1921 cggcaagctc cacctcggaa atggaggcca ccacccaggc caccacgtat gccaaggacg
     1981 tgatggagcg ggcgctgaac cgggtgatcg agcgaactcg gaccacccgg acggtcacta
     2041 ccatcatcga acatgaggac accaccgagc acggcttcac caatgtgcaa gtggaggacg
     2101 caccggcacc agagaacatc gcgggggtat atcgctggct ggacaaagtg tactacaaca
     2161 aggtggtcaa ctatggcaag cggttgctgt tcgaattcat catcccggag cctgccgctt
     2221 tccacatttt cagtgccatg gccaaaccgg ccagcgacca ggtccatgaa ttgccgccgc
     2281 cgttcgacat cgcatccttc aacgagatcc ggccggacac gtacgacgcg ttggtcatca
     2341 aatacggcgc cgtgggcgtc aagccgccac ccacgcccat cgtgtcggtg aacgaggtct
     2401 tctccgaaga acccacacct acaggcagtt ccaagttcaa cgcataccat gcgaacgtgc
     2461 agatccccgc agggtacaag gccatgcatg cgcgcgtctc cagcatttgg agccatgggc
     2521 aggatgtgaa cgggaactgg cactacgttt ggttcgccgt tgacactcag ccttacatct
     2581 ggcgctatca gagcggcgag gacgtgatcc cgctcagcca gcagaccggg accatcgcga
     2641 ttaacagcat cagttacacg
//
LOCUS       DAOQOC010000335         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21751_length_2658_cov_3.124088, whole genome shotgun sequence.
ACCESSION   DAOQOC010000335 DAOQOC010000000
VERSION     DAOQOC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_2658_cov_3.124088"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..647)
                     /locus_tag="PL106_06375"
     CDS             complement(30..647)
                     /locus_tag="PL106_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32729.1"
                     /translation="MRPLANALSVLLHPLWMTTGALVLCSRIDPFMFLDPGSQAGLIF
                     LGLVFLMTALFPLVSTLLMRRSGLVSDLTLPTRSERIPVYIIGLLYHGMCWYLVRRIT
                     DHPVTLGIVIGAFITLLLVLLITLYWKISAHMAGIGGLIGAVVAVMVLDGGEVTLALS
                     GLFVLAGALGTARLIVSDHTPAQVYTGTLLGFVCVFASAASGITT"
     gene            complement(723..2180)
                     /gene="rpoN"
                     /locus_tag="PL106_06380"
     CDS             complement(723..2180)
                     /gene="rpoN"
                     /locus_tag="PL106_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNA32730.1"
                     /translation="MLKQNLSLKLQQKLSPQQIQLMKMLQLPTVELEQRVKDELSENP
                     ALEEGEERDDAETAAEENTESLDQQEGEQDDDRDDIDLSDYFDDDTPDYKLQVQNKGR
                     DEEERETPLAGGNTFQDILRSQLALRDIDERTQLLGEHLIGNLDEDGYLRRELDAIVN
                     DLAFTANVTTTTEDLEAALKEVQALDPAGVGARDLRECLLLQLRRLPHAAETLTARAI
                     VDKHFDAFTKKHYDRIMERLEIDEDALKDAIAEIMRLNPKPGNTGRDSDKPVQEIVPD
                     FIINTVDGQLELQLNGRNAPELRVSRQYREMIAEYQRNKKDPAAQDALVFLKQKLDSA
                     KWFIDAIKQRQHTLLVTMEAIMEHQRDFFLTGDETRLKPMILKDIAEKVRMDISTVSR
                     VANSKYVQTNYGTILLKFFFSESLTNQEGEEVSTREVKKILKDAIEAEVKKNPLTDEE
                     LTELLKGKGYNIARRTVAKYREQMNFPVARLRKEL"
     gene            complement(2285..>2658)
                     /locus_tag="PL106_06385"
     CDS             complement(2285..>2658)
                     /locus_tag="PL106_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA32731.1"
                     /translation="GISYGWKRMQDNVESFKLLGSKSGLKQMGGLGSMAKAYGPRWIW
                     SRFWEMTAFLSMALAFLNILPIPALDGGHVIFLLYEAIARRPAPPKVLEYAQMAGMVF
                     LLTVIIAVNGHDLFKLFTGQL"
BASE COUNT          467 a          797 c          839 g          555 t
ORIGIN      
        1 gccacggaca gcggcgggaa acgccgcgct caggtggtga tacccgatgc tgcgctggcg
       61 aacacgcaga caaagccgag cagcgtgccg gtgtacactt gggcgggcgt gtggtcgctg
      121 acgatgagcc gggccgtacc gagcgcaccg gccagtacga agaggccgga aagagccagt
      181 gtgacctcac caccatcaag gaccatgacc gccaccaccg caccgatgag cccgccgatg
      241 cccgccatgt gcgcactgat cttccaatac agggtgatga ggagcacgag cagcagggtg
      301 atgaacgccc cgatcacgat gcccagcgtt accggatggt cggtgatcct acggaccagg
      361 taccagcaca tgccgtggta gaggagtccg atgatgtaca cgggtatgcg ctcggaccgc
      421 gtaggcagcg tcaggtcgct caccaggccg ctgcgccgca tgagcagtgt gctgacgagg
      481 gggaacaggg cggtcatcag gaagaccagg ccgaggaaga tcaggcccgc ctgcgatccg
      541 ggatcgagga acatgaacgg atcgatccgg gagcacaaca ccagggcccc ggttgtcatc
      601 cagagcggat gcaggagcac ggagagggcg ttggcgaggg gacgcatgtc aaacatcaag
      661 gctcaagacg cgatccatgc ctgatgtcgt ttgcggcttg agccttggaa tttgaagctt
      721 gctcagagtt ccttccgcag gcgagccact ggaaagttca tctgttcgcg gtatttggcc
      781 acggtgcggc gggcgatgtt gtaccccttg cccttcagga gctcggtcag ttcctcatcg
      841 gtgagggggt tcttcttcac ctcggcttca atagcgtcct tgaggatctt tttcacctcg
      901 cgggtgctca cctcctcgcc ttcctggttg gtgaggctct cgctgaagaa gaacttcaac
      961 aggatcgtgc cgtagttggt ctgcacatac ttgctgttgg ccacgcggct cacggtgctg
     1021 atgtccatgc gcaccttctc ggcgatgtcc ttcaggatca tgggcttcaa ccgggtctcg
     1081 tcaccggtga ggaagaaatc gcgctggtgc tccatgatgg cctccatggt gacgagcagg
     1141 gtgtgctggc gctgtttgat cgcgtcgatg aaccacttcg cgctatcgag cttctgtttc
     1201 aggaagacca gcgcgtcctg tgcggccggg tccttcttgt tgcgctggta ctcggcgatc
     1261 atctcgcggt actggcggct cacccggagt tccggagcat tgcgtccgtt gagttgcagt
     1321 tccaattggc catcgaccgt gttgatgatg aaatcgggca cgatctcttg taccggttta
     1381 tcggaatcac gtccggtgtt gcccggcttg gggttcaggc gcatgatctc ggcgatcgcg
     1441 tccttcagcg cgtcctcgtc gatctcgagc cgctccatga tgcggtcgta gtgcttcttg
     1501 gtgaacgcgt cgaaatgttt gtcgacgatg gccctggccg tgagtgtttc cgcggcgtgt
     1561 ggcaggcgcc tgagttgcag caacaggcac tcccgcagat cgcgtgcgcc aacccccgcc
     1621 ggatcgagcg cctgcacctc cttgagcgcg gcctccagat cctccgtggt ggtggtaacg
     1681 ttggccgtga aggccaggtc gttcacgatg gcatccagtt cgcgacgcag gtagccgtcc
     1741 tcgtcgaggt tgccgataag gtgctcgccc agcagttggg tgcgctcatc gatatcgcgc
     1801 agcgccaatt gcgatcgcag gatgtcctgg aaggtattgc cgcccgccag gggcgtctct
     1861 cgctcctcct cgtcgcgccc cttgttctga acctgcagct tgtagtcggg tgtgtcgtcg
     1921 tcgaagtagt cgctcaggtc gatgtcgtcg cgatcgtcgt cctgctcgcc ttcctgctgg
     1981 tccaggcttt cggtgttctc ctcggccgcg gtttcggcat cgtcgcgctc ctccccttct
     2041 tccagcgcgg ggttctccga cagctcgtcc ttgacgcgtt gctccaactc cacggtgggc
     2101 agttgcagca tcttcatcaa ttggatctgc tggggagaaa gcttctgctg gagcttgagc
     2161 gacaggttct gtttgagcat ggcaacgcgt acggtgcgaa aataggggcg gccacaaggt
     2221 tgcgggcgtg atccacatca cgcgctcatg accccgcccg cacgaggtcg ggaccatccg
     2281 tggatcagag ttgcccggtg aagagcttga agaggtcgtg cccgttcacc gcgatgatca
     2341 ccgtgagcag gaagaccatg ccggccatct gggcatactc cagcaccttg ggcggagcgg
     2401 gccttcgcgc gatggcctcg taaagcagga agatcacgtg gccaccatcg agtgccggga
     2461 tcggcaggat gttcagaaag gccagtgcca tgctcaagaa ggccgtcatc tcccagaagc
     2521 gcgaccagat ccagcgcggt ccgtacgcct tggccatgct gcccagccca cccatctgtt
     2581 tcaggccgct cttggagccg agcaacttga acgactccac attgtcctgc atgcgcttcc
     2641 agccataact gatgcccg
//
LOCUS       DAOQOC010000336         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21771_length_2657_cov_3.113759, whole genome shotgun sequence.
ACCESSION   DAOQOC010000336 DAOQOC010000000
VERSION     DAOQOC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21771_length_2657_cov_3.113759"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="PL106_06390"
     CDS             <1..1977
                     /locus_tag="PL106_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA32732.1"
                     /translation="GIQQFMVSDDVRERSVHSLRVTPSYGVVFGTSYGRIFRIAHGQV
                     EPLTSPGTRATGRDRVRQLEEGPDGRLWITTDSWFGTYDHGRDHTIPMRIQDSTRSDL
                     VVLGMKAFAIGVDGHVVSSFFGLGTVQQGPAGSETRFDKSLWKGSTRIYAPFIDSAGT
                     VWFDSGNRLLSSSNGLVKEFPALDAEFGLRITSITGMEEHTLAIGSAGGGVKLVRNGQ
                     VIRRFTTKEGLPSDEVRRVRYEQGTLLVATDHGAAMIHDPLGAAETLLFTLFEGLPSD
                     DVQDVDMDTASLWLATSEGLFAIPLRHTGHGLRPPITRILRVMVADRMISLDKPMRFD
                     LGTRLQVVAHGFAFSMPDAVEYAFRDDADTSWMSSASGVFSITLSEPGAHRLLVRSRL
                     PDTGWSPIVTLDVEVLSPWYLTWWFRGLAGVTLLCMGMVLVRYLARRRLRAHMLRMER
                     EKAQQEERRRIAADMHDDLGADISHLLLLARESAGSGTLDPQDRDNLMAVERYADGMV
                     RKIDEVIWSLAPEDDRLLPTLRFIQRHTEEIAGAHHLAFRTIPINGSDRPYPSMLRRE
                     LFLLVKESLANIIKHTDVRTLRFEAVVSEDRFSITIEDDGRPLVSGHPTRNGHGQANM
                     RHRLEKLSGEVRTEPVEPMGTRTIFRVWTGRSPQ"
     gene            2021..>2657
                     /locus_tag="PL106_06395"
     CDS             2021..>2657
                     /locus_tag="PL106_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA32733.1"
                     /translation="MSDTPRASGLRILIVEDHDDFRHRLSALLSSYPEFSVRAVPDAQ
                     SALRAFEVGAHEVVIMDINLQPATGQGSSSFESMNGIACTRAIKQKHPEVQVLICTVH
                     EDEERIFEALKAGASGYILKRAPLPELIEAIHQVHRGESPMTGTIARKVVSSFQAKPL
                     QCGEKLTTQEQKVLDLLAEGLKAREIAEKLFISITTVRSHVRGIYEKLQVQN"
BASE COUNT          532 a          769 c          848 g          508 t
ORIGIN      
        1 ggaatacagc aattcatggt cagcgatgat gttcgggaac gatcggtcca ctcactgcgc
       61 gtaacaccga gctacggcgt ggtcttcggc acctcctacg gtaggatatt ccggatcgca
      121 cacggacagg tggagccgct caccagcccg ggcacacgcg ccacggggcg ggatcgtgtg
      181 cggcaactgg aggaaggacc ggatggaaga ctctggatca ccacggacag ttggttcggt
      241 acatacgatc atggccggga tcacactatc ccgatgcgca tccaggattc gacccgttcg
      301 gacctcgtgg tcctgggtat gaaggcattc gcgatcggtg tggatggcca tgtggtctcc
      361 agctttttcg gcttgggcac ggtgcaacag ggaccggccg gatcggagac acgctttgac
      421 aagtcgttat ggaagggcag cactcgcatc tacgcaccat tcatcgacag cgccggtaca
      481 gtgtggttcg attcgggcaa ccgattgctg agtagttcga atggcctggt gaaggagttt
      541 cctgcattgg atgcggagtt cggcctgcgc atcacaagca tcaccggcat ggaggagcac
      601 acactggcca tcggatcggc cggtggaggg gtgaaactcg tgcgcaacgg acaggtgatc
      661 aggcgattca ccaccaagga aggcctgccg agcgacgagg tacgccgagt gcggtacgaa
      721 caaggaacac tgctcgtggc cactgatcat ggagcggcga tgatccatga tccgttggga
      781 gcggcggaaa cgttactgtt cactcttttc gagggactgc cttccgatga tgtacaggat
      841 gtggacatgg atactgcctc gctctggttg gccacttccg aggggttgtt cgcgataccg
      901 ctccggcaca ccggccacgg gttgcgcccg ccgatcacac ggatcctgcg cgtcatggtg
      961 gccgatcgaa tgatctccct cgacaagccg atgcgtttcg acctgggtac ccgccttcag
     1021 gtagtggcgc acggattcgc attcagcatg cccgatgctg tggagtacgc ttttcgggat
     1081 gatgcggata catcctggat gtcgagtgcc tctggcgtgt tcagtatcac cttgtcggag
     1141 ccgggcgcac accgactact ggtgcggtcc cgcttgcccg ataccggatg gagtccgatc
     1201 gtcacactgg atgttgaggt gctgagcccg tggtacctaa catggtggtt ccgtggtctc
     1261 gcaggggtca ccctgttgtg catgggcatg gtgctggtgc ggtacctcgc ccgccgccgc
     1321 ctgcgtgcgc acatgctgcg catggagcgc gagaaagcac agcaggaaga gcgacgccgg
     1381 atcgcggcgg acatgcacga tgacttggga gcggacatca gtcacctgtt gctccttgcg
     1441 cgcgagagcg ctgggagcgg caccctggac ccgcaggacc gggacaactt gatggccgtg
     1501 gagcggtatg ctgacggcat ggtgcgcaag atcgacgagg tgatctggag cctggcccct
     1561 gaggacgacc gcttgctccc tacgttgcgc ttcatccagc gtcacaccga ggagatcgcc
     1621 ggagcccatc acctcgcctt ccgtacgatc ccgatcaatg ggtccgatcg cccgtatcca
     1681 tccatgctcc gtcgggagct ctttctcctg gtgaaggaat cgctcgccaa catcatcaaa
     1741 cataccgatg tgcgcaccct gcgattcgag gctgtggtct ccgaggatcg gttcagcatc
     1801 accatcgagg acgatggtcg gccgctggta tcggggcatc cgacccgcaa tggtcatgga
     1861 caggcgaaca tgcggcaccg actggagaaa ctctctggtg aagtgcgcac cgaacccgtg
     1921 gaaccgatgg gtacgcgaac gatcttccgg gtgtggacag gacgttcccc tcaatagtga
     1981 tgatgggatc cggcggaccg gccggtaatt ttcccgccgt atgtccgaca caccccgcgc
     2041 ttccggtctg cggatcctca ttgtggagga ccacgacgac ttccgccatc gactgagcgc
     2101 cctgctctcc tcctatccgg agttcagtgt acgtgcggtg cccgacgcgc agagtgcatt
     2161 gcgggccttc gaggtgggtg cgcacgaggt ggtgatcatg gacatcaacc tgcagcctgc
     2221 gaccggccag gggtcttctt cattcgagag tatgaacggc atcgcatgca ccagggctat
     2281 caagcagaag cacccggaag tgcaggtgct gatctgcacc gtgcatgagg atgaagagcg
     2341 gatattcgag gccttgaagg ccggggcgag cgggtacatc ctcaagcgag cacccctacc
     2401 cgaacttatc gaggcgatcc accaggtgca tcgtggcgaa agtccgatga ccgggaccat
     2461 tgcacgaaag gtggtcagca gcttccaagc gaagccgctg cagtgcgggg agaaactcac
     2521 gacccaggag cagaaggtgc tcgacctgct tgcagaaggt ctcaaggcgc gcgagatagc
     2581 cgagaagctg ttcatcagca tcaccacagt gcgcagccac gtgcgcggca tctacgaaaa
     2641 actgcaggtg cagaacc
//
LOCUS       DAOQOC010000337         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21796_length_2655_cov_3.685000, whole genome shotgun sequence.
ACCESSION   DAOQOC010000337 DAOQOC010000000
VERSION     DAOQOC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21796_length_2655_cov_3.685000"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PL106_06400"
     CDS             complement(<1..479)
                     /locus_tag="PL106_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32734.1"
                     /translation="MASTTRSTRLGRAHTPLLLALAWKNVRLRYKSSMLGFVWTLLNP
                     LIFLGIFLLIFSNAFPQVPNYAVFALTGLVFWSFFATVSGHVLGALVENASILKSLAV
                     PPLAFPLGQLLAGLFNLLLSFVPLAAILVWFGWRPAPQHLLIVPATALFALFVFGIGL
                     "
     gene            complement(748..1020)
                     /locus_tag="PL106_06405"
     CDS             complement(748..1020)
                     /locus_tag="PL106_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32735.1"
                     /translation="MAKEDGTSATNHQQRMEHKVILHIEKLPEGVYLATSDSVQGLVA
                     QGRTIAETLEIARDVAKKPLEAQAPGSLPQTVEFGDRFEYPLVVGL"
     gene            1119..1367
                     /locus_tag="PL106_06410"
     CDS             1119..1367
                     /locus_tag="PL106_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32736.1"
                     /translation="METLRVKVPTKKAKALLEQLDGLGVIEIEHERDDASLETLVATM
                     KRIRAGIKDKMTVKEVVAEVDAVRSKRYAASRKAQTRR"
     gene            1333..1749
                     /locus_tag="PL106_06415"
     CDS             1333..1749
                     /locus_tag="PL106_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNA32737.1"
                     /translation="MPHPGRLRLVVDTNIISYLLTSSFTSLVNGWRTGRFVVLFSEPQ
                     INELLDVLSRPRIRNRVPERIANTFIKALRRNATRVETTSIVDVCRDKKDNFLLALAQ
                     DGLADLLITGDENLLVVERFGGTIILSPSEFTSAFN"
     gene            1780..>2655
                     /locus_tag="PL106_06420"
     CDS             1780..>2655
                     /locus_tag="PL106_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNA32738.1"
                     /translation="MSLVINGRFLARPVTGTERYGRLLLRLIAQAWPDSRVIVPKGSP
                     EKLDACGLAVERVGNTSGHVWEQMELPRAAGKGDVLLSPANSGPLSVARHVPVVHDLA
                     YLHHPEWFNSRFATWYKLLIPRMLRRAERVITVSGTVQQELQRYYGIGPDKSFVVPPY
                     VLPSLLPANGSAPEQCPYYLMVASLDPRKGIDRVLNWYTGLHRPAFDLLIVGRKHRAF
                     APVTIPDHPGLHILNDVDDARLGGLYRDAIALIQPSYYEGFGLPILEAITIGTPVITG
                     HLNVFTEQFGDAVIET"
BASE COUNT          532 a          836 c          792 g          495 t
ORIGIN      
        1 aggccgatgc cgaagacgaa cagcgcgaac agcgcggtgg ctggtacgat cagcaggtgc
       61 tgcggtgcgg gtcgccatcc gaaccacacg aggatggccg ccaacggcac gaaactcagc
      121 agcaggttga acagccccgc gagcaactgc cccagcggga aggccagcgg cggcaccgcc
      181 aggcttttca gaatcgaagc gttctctacc aaagctccca gcacgtgccc gctcaccgtg
      241 gcgaagaagg accagaacac caggccggtg agcgcgaaga ccgcatagtt cggtacctgt
      301 gggaaggcgt tgctgaagat gagcagaaat atacccagaa atatcagcgg attcaacagg
      361 gtccacacga aaccgagcat cgaactcttg tagcgcagcc gcacgttctt ccacgccaag
      421 gccagaagca gcggcgtatg ggcgcggcca aggcgggtgc tccgggtggt gctggccatc
      481 ggtgggcgtg caaggtagaa gctcgtggcg gtggtcggcc ctaccgcact ttcaggaatt
      541 cttccggtgc cacatccgcc tgcttgagaa tggcccgtaa cgtaccttcc ggcatgtcac
      601 ccgcatgctt ggcgatcgtg gtgaaacgac cggaacccgg acaatgctgg atctcgtggc
      661 tcccagccgc ctgacgatgg aactcggatc cgaatgatcg gagcctgcgc acaatgtcgc
      721 ggtagctgga tccgccgagc cttcccatca cagacccacc accagcggat actcgaagcg
      781 gtcgccgaac tccacggtct gcggcagcga tccgggagct tgggcttcca gcggcttctt
      841 ggcgacatca cgcgcgatct ccagcgtttc cgcgatggtg cgcccttgcg caaccaggcc
      901 ctgaaccgag tctgacgtag ccaggtatac gccttcaggc agcttttcga tgtgcaggat
      961 gaccttgtgt tccatgcgtt gctgatggtt cgtcgcagag gttccgtctt ccttcgccaa
     1021 tggtacggca tgtgcatggt cggggcggct gcctcgcaca gtgctggcca tcggaggacg
     1081 tgcaaggtgg atccggccgc ccaacgtagc tttacgccat ggaaaccctg cgggtgaagg
     1141 tgcccaccaa gaaggccaag gccctgctcg aacagttgga cggacttggc gtgatcgaga
     1201 tcgagcacga acgcgatgat gcctcgttgg agaccctcgt cgccaccatg aagcgcatcc
     1261 gtgccggtat caaggacaag atgacggtga aagaggtggt ggccgaagtg gacgccgtta
     1321 gatccaagcg ttatgccgca tcccggaagg ctcagactcg tcgttgatac gaacatcatc
     1381 agctacctgc tgaccagttc gttcacatcc ctggtgaacg gctggcggac cgggcgcttc
     1441 gtcgtgctgt tctcggaacc gcagatcaat gaactactgg acgttctttc gcgaccgcgc
     1501 atccggaacc gtgtgcccga aaggatcgcg aacaccttca tcaaggccct tcggcggaac
     1561 gccacccggg tggagaccac cagcatcgtg gacgtgtgcc gcgacaagaa ggacaacttc
     1621 ctgctggccc tggcccagga cggcctggcc gacctgttga tcacgggtga tgagaatctg
     1681 ctcgtcgttg agcggttcgg cggaacgatc atcctttcgc catcggagtt cacctcagca
     1741 ttcaactgac caacaagccc tcctgcacct cacaacagca tgtcgctcgt catcaacggc
     1801 cgctttctgg ccaggccggt cacgggcact gaacgctacg gaagattgtt gctgcgtttg
     1861 atcgcgcagg cctggcccga tagccgcgtg atcgttccca aaggttcacc ggagaaactc
     1921 gatgcctgcg ggctcgcggt ggaacgcgtg ggcaacacct ccggccacgt ttgggagcaa
     1981 atggaattgc cccgtgctgc aggtaaaggc gatgtgctcc tctccccggc caactccggt
     2041 ccgctgagtg tcgcacgtca tgtgcccgtg gtacacgacc ttgcctacct gcatcacccg
     2101 gaatggttca atagccggtt cgccacctgg tacaaattgc tgatcccgcg catgctgcgc
     2161 cgcgctgaac gtgtgatcac cgtgagcggc accgttcagc aggaactaca gcgctactac
     2221 ggcattgggc ctgacaagtc gttcgtggtg ccgccctatg tgctcccatc cttgctcccg
     2281 gccaacggga gcgcaccgga acaatgtcct tactacctga tggtcgcctc cctcgatccg
     2341 cgcaagggca tcgatcgcgt actcaattgg tacaccggtc tgcatcggcc ggcattcgac
     2401 ctgttgatcg tgggccgcaa gcatcgcgcg ttcgcaccgg taacgatccc cgaccatccg
     2461 ggtctgcaca tcctgaacga cgtggatgat gctcgcctcg gtggtctgta ccgcgatgcc
     2521 atcgccttga tccaaccctc ctactacgaa ggattcggac tgccgatcct ggaagccatc
     2581 accatcggca ccccggtgat caccgggcac ctgaacgtct tcacggagca gttcggcgat
     2641 gcggtgatcg agacc
//
LOCUS       DAOQOC010000338         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21855_length_2651_cov_3.246533, whole genome shotgun sequence.
ACCESSION   DAOQOC010000338 DAOQOC010000000
VERSION     DAOQOC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21855_length_2651_cov_3.246533"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PL106_06425"
     CDS             <1..979
                     /locus_tag="PL106_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA32739.1"
                     /translation="GEATTLQAFTSWRWRWNEQWTMTSGVHVLHFALTNATSVEPRAG
                     IRYQMKPGRAFTAGAGLHAKTETAMTYLAQVTDQQGHTSRPNRMLGLSKAAHVVVGYE
                     HMITEDIQVKAEAYYQYHFNVPVENDPTSAFTLSNSSDWFTNKPLVNKGKGRNYGIEL
                     AVEKYFTRGYHFMVTASLSDARDKALDGVWRNSRFNMGAVANALAGKEWQMGRNGKDR
                     VLTTGFRYSIIGGQYSTPIDLEASRLAGTQVDGGTPWSRKGAPVQKLDVVVAYRAVRP
                     KVSHEIKADVQNVLNSSTAVYHYYDNRTKAIGTVPQLALLPVLQYTLRF"
     gene            complement(1098..2408)
                     /gene="rimO"
                     /locus_tag="PL106_06430"
     CDS             complement(1098..2408)
                     /gene="rimO"
                     /locus_tag="PL106_06430"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNA32740.1"
                     /translation="MKARTLKRTKVNVITLGCSKNTVDSEVLMAQLKANGIAVDHEAA
                     HDDHSVVVINTCGFIENAKQESIDTILSWARAKDKGQVEKVYVTGCLSQRYAPELKEG
                     IPQVDAWFGTRDLPRLLKTLKADYKHELVGERLLTTPAHFAYFKISEGCDRKCSFCAI
                     PLMRGGHVSVPMEQLVTNARGLAARGVKELILIAQDLTYYGLDIYKKRNLDELVSRLS
                     DVEGIDWIRLHYAFPSGFPMDVLDVMRDRPNVCNYLDMPLQHGSTRMLTRMRRGITQE
                     KTEKLVSDIRSRVPGIAIRTTLIAGFPGETEADHEDSLRWIERMRFDRLGCFTYSHEE
                     DTHAFGMEDDVPFGVKQERANAIMELQGGISFELNQRKVGQEYRVLVDKAESGNYHAR
                     TEHDSPEVDNDVLIATSSGHLRLGDFAQVRITEAREHELVAVPV"
BASE COUNT          477 a          846 c          815 g          513 t
ORIGIN      
        1 gggtgaggcg accacgttgc aggccttcac cagttggcgc tggcgctgga atgagcaatg
       61 gaccatgacc agcggggtac acgttctgca cttcgcactg accaatgcca cgagcgtgga
      121 gccgcgtgcc ggtatccggt atcagatgaa gccgggccgt gcgttcaccg cgggtgccgg
      181 cctgcatgcg aagaccgaaa ccgcgatgac gtatctcgcc caggtcaccg atcaacaggg
      241 ccatacctcc cgccccaaca ggatgctcgg gctttcgaag gccgcgcatg ttgtggtggg
      301 ctatgagcac atgatcacgg aggacatcca ggtgaaggcc gaggcgtact accagtacca
      361 cttcaacgtg ccggtggaga acgatcccac aagcgcgttc accttgagca acagctccga
      421 ctggttcacc aacaaaccat tggtgaacaa gggcaagggc cgcaactacg ggatcgagct
      481 cgcggtggag aaatacttca cgcgcgggta ccatttcatg gtcaccgcct cgttgagcga
      541 cgcgcgcgac aaggctctgg acggggtctg gcgcaattca cgcttcaaca tgggtgccgt
      601 ggcgaacgcc ctcgcgggca aagaatggca aatgggcagg aacggcaagg accgcgtgct
      661 caccaccgga ttccgctact cgatcatcgg tggccagtat tccaccccca tcgatctaga
      721 ggcgagccga cttgcgggca cgcaagtgga tggcggaaca ccctggagcc ggaagggcgc
      781 accggtgcag aaactcgatg tcgttgtggc ctaccgcgct gttcggccaa aggtgagcca
      841 cgagatcaag gcggatgtgc agaatgtact gaacagcagc acggcggttt accactacta
      901 cgacaaccgc accaaggcca ttggaaccgt gccgcagttg gcattgcttc ccgtgttgca
      961 gtacacgttg aggttctgaa ccgtcgcgga atgaacggac cggacccggc gatcgcgtga
     1021 ccggagccgt gcggatggcc ccccttcatg ctggagggcg gccatcttgt tcaagctcac
     1081 cggccggagt ggtcggttca aaccggcacc gccaccaact cgtgctcgcg cgcttccgtg
     1141 atgcgcacct gcgcgaagtc gcccagacgc agatgaccgg acgatgtggc gatgagcacg
     1201 tcgttgtcca cctcgggcga atcgtgctcg gtgcgggcgt ggtagttgcc gctctcggcc
     1261 ttgtccacca gcacgcggta ctcctgcccc accttgcgct ggttgagttc gaagctgatg
     1321 ccgccctgca gttccatgat cgcgttggcg cgctcctgtt tcaccccgaa gggcacatcg
     1381 tcctccatgc cgaaggcgtg cgtgtcctcc tcgtggctgt acgtgaagca gcccaaccga
     1441 tcgaaccgca tgcgctcgat ccagcgcagg ctgtcctcgt ggtcggcttc ggtctctccc
     1501 gggaagcccg cgatgagcgt ggtgcgtatg gcgatgccgg gcaccctgga gcggatgtcc
     1561 gataccagtt tttcggtctt ctcctgcgtg atgccgcgtc gcatgcgggt gagcatgcga
     1621 gtgctgccgt gctgcagcgg catgtccagg tagttgcaca cgttgggccg gtcccgcatc
     1681 acgtccagca cgtccatggg gaatccactg ggaaaggcgt agtgcaggcg gatccagtcg
     1741 atgccctcca cgtcgctcag ccgggagacc agttcatcga ggttgcgctt cttgtaaatg
     1801 tccagtccgt agtacgtcag atcctgtgca atgaggatca gttccttcac accccgtgcg
     1861 gccagacccc tggcgttggt caccagctgt tccatcggta cgctcacatg cccgccgcgc
     1921 atcaatggaa tggcgcagaa ggagcacttg cgatcgcagc cttcactgat cttgaagtag
     1981 gcgaagtgcg cgggcgtggt gagcaggcgc tcccccacca gttcgtgctt gtagtccgcc
     2041 ttgagtgttt tcagcaagcg cggcagatca cgtgtcccga accaggcgtc cacctgcggg
     2101 atgccttctt tgagctcggg cgcgtagcgc tggctcaggc agccggtgac atacaccttc
     2161 tccacctgtc ccttgtcctt ggcccgggcc caactgagga tggtatcgat gctctcctgc
     2221 ttggcgttct cgatgaagcc gcacgtgttg atcaccacca cgctgtggtc atcgtgcgcc
     2281 gcctcgtggt ccaccgcgat gccgttggct ttcaattggg ccatcaggac ctcgctgtcc
     2341 acggtgttct tcgaacagcc gagggtgatc acgttgacct tcgtgcgctt gagggtgcgg
     2401 gctttcatgt tcggccgcga aggtccggct tccgggcgaa accccgatca gccatctatt
     2461 ttcgccgaac acaatgtgcc ccgccatgcg cctgatcgct gcttccctcc ttctgaccag
     2521 cctgttcgcc tgctcttcct gcagggatga tccggcccag aacaccagca cgcccccgga
     2581 gaccgcgaac gctgcggcga atgccacccc cgggggggcc ggttgcccgc ccgcgcgtac
     2641 acccgacgac t
//
LOCUS       DAOQOC010000339         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21896_length_2648_cov_4.145006, whole genome shotgun sequence.
ACCESSION   DAOQOC010000339 DAOQOC010000000
VERSION     DAOQOC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21896_length_2648_cov_4.145006"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PL106_06435"
     CDS             <1..794
                     /locus_tag="PL106_06435"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA32741.1"
                     /translation="EAAHLLAFVNLVLLFYMRDVARRPVRFALMLAFYLLNLAIIMSV
                     NSRLSFMVFFVMTVLVLVRAVRAVRGLYWTSLFTMPIMVSFALLIYEVLSLPFFTALL
                     SRVDKKDVTTYNGRTYIWESAWDWATTDGRGLLLGNGYNGQYRLHLLEYVAKLWGADA
                     SYNLHMHSAFLEFFVNQGIVGVLLMYWLYWQAMKHCYDHYRRNTAMAPLYGGLLYLLF
                     VWQIDITGYGYYLGFMLLLMIMAPFSIKASAITGKRTDLDGRYLS"
     gene            791..1627
                     /locus_tag="PL106_06440"
     CDS             791..1627
                     /locus_tag="PL106_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA32742.1"
                     /translation="MNIRQGGRRDRGIGPPTGDHPLVSIVTVVYNGATTIERTILSVL
                     SQTWPRIEYIIVDGGSTDGTLDILRRYEDRIDLWQSSPDKGIYDAMNKGVALCTGDWV
                     GLINADDWYPDDAVATAMTAAGERQGINIVHGDILIHYPNGTSKVKHPKVSGFLLKYW
                     EMTLNHPGFFVRRSFYTGRPFNTTFRVSGDHHWTLRAWLEGPHMFLRVPAVLAHFSAG
                     GASGAVPLAKGLREGDAIGHDLGFSAWDRFLGRVVRTALHVPAQAKLWFNQFVAPLLR
                     RK"
     gene            1624..2130
                     /locus_tag="PL106_06445"
     CDS             1624..2130
                     /locus_tag="PL106_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNA32743.1"
                     /translation="MMASPFHIFQDVRANRNNPKGLLVMTFFRLMHLLRRSTITFILF
                     IPLFVLYRVLVEWILCIELPWKTRLGPGFRIDHGQALIINDGTVFGSGCIVRNSTTIG
                     NRKLKDGTFSRPPIFGDRVDIGANAVIIGPITIGDDVAIGAGAVVLKDVPSKHVAVGN
                     PARIIPRP"
     gene            complement(2205..>2648)
                     /locus_tag="PL106_06450"
     CDS             complement(2205..>2648)
                     /locus_tag="PL106_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32744.1"
                     /translation="QVQANDPLEKQMLLLNDGASEQTFELDGCWSDVNGQYWSGSISL
                     PGFGSKVLVRESDDLCDMSTGVEDAPIQVGSAGIYPNPVAPGARINFSAPAEGPLQFV
                     GANGQELASVVLPPGTMGMDLPKILGRGVYALRMSGRPVERVVVE"
BASE COUNT          497 a          829 c          769 g          553 t
ORIGIN      
        1 acgaagcagc ccacttgctg gccttcgtga acctggtgct gctcttctac atgcgcgacg
       61 ttgcccgccg tcccgttcgt ttcgccctca tgttggcctt ctatctgttg aatttggcga
      121 tcatcatgag cgtgaacagc cgcctctcct tcatggtctt cttcgtgatg accgtgctgg
      181 tactcgttcg cgcggtgagg gccgtacgcg gattgtactg gacctcgttg ttcaccatgc
      241 ccatcatggt cagcttcgcg ctgctgatct acgaggtcct ctcgttgccc ttcttcaccg
      301 ccctgctctc ccgtgtggac aagaaggacg tgaccacgta caacggtcgc acgtacatct
      361 gggagtcggc atgggactgg gccaccaccg atggccgtgg tctgctgctc ggcaacggct
      421 acaacgggca ataccgcctg cacctgctcg aatatgtggc caagctctgg ggagcggacg
      481 cctcgtacaa cctgcacatg cacagcgctt ttctggaatt cttcgtgaac cagggtatcg
      541 tgggggtcct gctcatgtac tggctttact ggcaggcgat gaagcattgt tacgaccatt
      601 accgccggaa cacggcgatg gcccccttgt acggtgggct gctgtacctg ttgttcgtct
      661 ggcagatcga cataacgggt tatggctact acctcggctt catgctgcta ttgatgatca
      721 tggccccatt ttcgatcaaa gcctccgcga tcaccggaaa acggaccgac ctggacggcc
      781 ggtatctctc atgaatatca ggcagggcgg tcgtcgggac cggggcatcg ggccacccac
      841 cggggatcac cccctggtga gcatcgtcac cgtggtgtac aacggcgcga ccaccatcga
      901 gcggaccatc ctgagtgtgc tgtcccagac atggccgcgc atcgagtaca tcatcgtgga
      961 cggcggcagc accgatggga cactcgatat cctgcgccgc tacgaggaca ggatcgatct
     1021 gtggcaaagt tccccggaca aggggatcta cgacgcgatg aacaaaggtg tcgcgctgtg
     1081 taccggcgat tgggtcggcc tgatcaatgc ggacgactgg tatcccgatg atgcggtcgc
     1141 aacggccatg acggcggccg gggaacggca agggatcaat atcgtgcatg gcgacatcct
     1201 gatccattac cccaatggca ccagcaaggt gaagcacccg aaggtgagcg gcttcctgct
     1261 caagtactgg gaaatgacgc tgaatcaccc gggcttcttc gttcggcgct ccttttatac
     1321 cggccgaccg ttcaacacca cgttccgtgt gagcggcgat catcactgga cactgcgcgc
     1381 ctggttggaa ggcccgcaca tgttcctccg ggtccccgcc gtactggccc atttcagcgc
     1441 ggggggcgcc agtggggctg ttcccttggc caagggcctg cgcgaagggg atgccatcgg
     1501 tcacgatctg gggttcagcg cgtgggatcg tttcctgggc cgcgtcgtgc gaacggccct
     1561 gcacgttccc gcacaggcca agttgtggtt caaccagttc gtggcacctt tactgcgccg
     1621 aaaatgatgg ccagcccctt ccatattttc caggacgtcc gtgccaaccg gaacaatccc
     1681 aaggggttgt tggtgatgac gttcttccga ctgatgcact tgttgcggcg cagcacgatc
     1741 actttcatcc ttttcatacc actgttcgta ctctaccggg tcctggtgga atggatcctg
     1801 tgcattgaac tgccttggaa gacacgactg gggccgggat tccggatcga ccatggccag
     1861 gccctcatca tcaacgacgg taccgtgttc ggctccggat gcattgttcg caacagtacc
     1921 accatcggca accgcaaatt gaaggacggc accttcagtc gtccgcccat cttcggtgac
     1981 cgggtggaca tcggcgcgaa cgcggtcatc atcgggccca tcaccatcgg cgatgatgtg
     2041 gccatcggcg caggtgccgt agtgctgaag gatgtgccct cgaagcacgt ggcggtgggc
     2101 aaccccgcgc gcatcatccc cagaccctga ccggttcctc acgagaacgc gaaggcccgg
     2161 tgcatgttcc gcaccaggcc ttcggatctc ctcgcctgcg ggggtcattc caccaccacc
     2221 cgttcaacgg ggcgacccga catgcgcagt gcgtagaccc ctcggcccag tatcttgggc
     2281 aggtccatgc ccatggttcc cggaggtagc accaccgagg caagttcctg tccattggcg
     2341 ccaacgaact ggagcgggcc ttcagcaggg gctgagaaat tgatcctcgc gcccggagcc
     2401 accggattgg gatagatccc ggccgaaccg acctgaatgg gcgcatcctc aacaccagtg
     2461 ctcatatcgc acaggtcatc gctctcacgc acgagcactt tcgaaccgaa accgggcagc
     2521 gaaatactac cggaccagta ctggccgttc acgtcgctcc agcatccatc cagctcaaag
     2581 gtctgctcac tggcaccatc gttgagcagg agcatctgct tctcgagggg atcattggcc
     2641 tgcacctg
//
LOCUS       DAOQOC010000340         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_21949_length_2645_cov_3.055598, whole genome shotgun sequence.
ACCESSION   DAOQOC010000340 DAOQOC010000000
VERSION     DAOQOC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21949_length_2645_cov_3.055598"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PL106_06455"
     CDS             <1..367
                     /locus_tag="PL106_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32745.1"
                     /translation="TLNPMTLGNTPMAIDLTQTSTSTYGTNARKSVSGAFPAQVLWAG
                     DVTYNGQVKYTGGGNDRDPVLITVGSTTPNNSANAYSTRDVNLNGQVKYTGSSNDRDP
                     ILLNVGSATPNNVRSEQLP"
     gene            414..1616
                     /gene="kbl"
                     /locus_tag="PL106_06460"
     CDS             414..1616
                     /gene="kbl"
                     /locus_tag="PL106_06460"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNA32746.1"
                     /translation="MSISTTTPFYRHIQQELDSIREAGLFKRERVITSEQGAEIMVNG
                     RTALNFCANNYLGLSSHPDVIAAAHRTLDSHGYGMSSVRFICGTQDIHKELEQKLAQF
                     HGTEDTILYAACFDANGGVFEPLLQETDAIISDSLNHASIIDGVRLCKAMRYRYANND
                     MADLELQLKTARKDGARHIIVVTDGVFSMDGIVADLKGVCDLAEKYQALVMVDECHAA
                     GFIGKTGRGSVEACGVSGRVDIITGTLGKALGGAMGGYTTGRKEIIEMLRQRSRPYLF
                     SNSLTPAIVGASIAVVDLLMKSTELRDKLERNVARFKNGIEALGFRTRGDRSAIVPVM
                     LGDAKLSQVMADKLLDEGIYVIGFFYPVVPKDTARIRVQLSAAHSEAHVDHALAAFGK
                     VGKELGVV"
     gene            1732..>2645
                     /locus_tag="PL106_06465"
     CDS             1732..>2645
                     /locus_tag="PL106_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNA32747.1"
                     /translation="MSIITRRKKSLVVLTALGASVSFFAFQPFELPNAGSAVPVSTFA
                     NFESAHVHPLDMTADGAKLLAVNTANNTLEVFQLGGPMLLNTASIPVGLDPVTVRVRT
                     NTEAWVVDQVSDEISIIDLATNRVVRSLITEDEPADVVFAQNKAFVSCAGRESIQVFN
                     LADLNAAPTEVLLKGEQPRALAVSPDGNTVYCAFFESGNATTVLNGNDFFSFAHGSNN
                     GVCSPQGGCTQIPNDVLNPNGPYGGAVDASAGIVPNAGTGFKPPLNPVNPPTTDSKSL
                     IVRKNASGQWLDDNGGDWTNMVSGSAAG"
BASE COUNT          528 a          841 c          833 g          443 t
ORIGIN      
        1 gacgctgaac ccgatgacgc tgggtaatac gcccatggcc atcgacctca cccagaccag
       61 cacctcgacc tacggcacca acgcgcgcaa gagcgtgagc ggagcctttc ctgcacaggt
      121 gctctgggcg ggcgatgtca cgtacaacgg ccaggtgaag tacacgggtg gtggcaacga
      181 ccgcgatccc gtcctgatca cggtgggcag caccaccccc aacaacagcg cgaacgcgta
      241 cagcacccgc gatgtgaacc tgaatggcca ggtgaagtac acgggcagca gcaacgaccg
      301 cgatccgata ttgctgaacg tcggcagcgc cacgccgaac aacgtgcgtt cggaacaatt
      361 gccgtagggg tcgtccgcgt ttgatacgtt cgcgctccca ttccaccgac cccatgagca
      421 tctcgaccac cacgcccttc taccgccaca tccagcagga actcgattcc atccgcgagg
      481 cggggctctt caagcgcgag cgcgtcatca ccagtgagca gggtgccgag atcatggtga
      541 acgggcgcac agcgctgaac ttctgcgcga acaactacct cggcctgtcc tcgcatcccg
      601 acgtgatcgc tgcggcgcat cgtacgctcg attcgcacgg ctacggcatg agcagcgtgc
      661 ggttcatctg cggcacgcag gacatccaca aggagctcga gcagaaactc gcgcaattcc
      721 acggtacgga agataccata ctgtatgcgg cctgcttcga tgccaatggc ggggtattcg
      781 agcccttgtt gcaggagacc gacgccatca tcagtgattc gctcaaccac gccagcatca
      841 tcgatggggt gcggctgtgc aaggccatgc gctaccggta cgccaacaac gacatggccg
      901 atctggagct gcagctgaag accgcgcgca aggacggggc ccgacacatc atcgtggtca
      961 ccgacggcgt cttcagcatg gacggcatcg tggccgatct gaagggcgtg tgcgatctgg
     1021 ctgaaaaata ccaggcattg gtgatggtgg acgagtgcca cgcggcgggc ttcatcggga
     1081 agacgggccg tggcagtgtt gaagcgtgcg gcgtatcggg gcgtgtggac atcatcacgg
     1141 gcaccttggg caaggcgctg ggcggggcca tgggcggtta caccaccggg cgcaaggaga
     1201 tcatcgaaat gctgcggcag cgctcgaggc cctacctctt cagcaactcg ctcacgcccg
     1261 ccatcgtggg tgccagcatc gctgtggtgg acctgctgat gaaaagtacc gaattgcgcg
     1321 acaagttgga gcggaatgtg gcccggttca agaacgggat cgaggcgctc ggtttcagga
     1381 cccgcggcga tcgcagcgcc attgttccgg tgatgctggg cgatgccaaa ctcagccagg
     1441 tgatggccga caagctgctg gacgagggca tctacgtcat cggcttcttc taccccgtgg
     1501 tgcccaagga caccgcccgc atccgggtgc agttgagcgc cgcgcacagc gaggcgcacg
     1561 tcgatcacgc gttggcggcc ttcgggaaag tggggaagga attgggggtg gtctgagggg
     1621 tgggggtttg caccgttcgc cgatcggact atcttgggct gcccgttccc gttccgcccg
     1681 tctgggtgga acgaccttgt cgccctccaa ggtaaccaga tccgctccac catgtccatc
     1741 attacccgcc ggaaaaagtc ccttgtcgta ctgaccgccc ttggcgcgtc ggtatcattc
     1801 ttcgcgttcc agcccttcga gctgcccaac gcgggctcgg ccgtgccggt gagcaccttc
     1861 gccaacttcg agagcgccca cgtacacccg ctggacatga cggcggacgg cgccaagctc
     1921 ctggcggtga acacggccaa caacaccttg gaggtcttcc agctcggcgg ccccatgttg
     1981 ctgaacaccg ccagcatacc ggtgggtctg gacccggtca ccgtacgggt gcgcacaaat
     2041 accgaggcgt gggtggtgga tcaggtgagc gacgagatca gcatcattga tctggccacc
     2101 aaccgcgtgg tgcgcagcct gatcaccgag gacgaaccgg ccgatgtggt cttcgcccag
     2161 aacaaggcct tcgtctcgtg cgcgggccgc gagagcatcc aggtgttcaa cctggccgat
     2221 ctgaatgcgg cgccgacaga ggtattattg aagggcgaac aaccccgtgc gctggccgtg
     2281 agccccgatg ggaacacggt gtactgcgcc ttcttcgaga gcggcaacgc caccaccgtc
     2341 ctgaacggca acgacttctt cagcttcgcc cacgggagca acaatggcgt gtgctcgccg
     2401 cagggcggct gcacgcagat cccgaacgac gtgctcaatc ccaacggacc ttacggcggt
     2461 gccgtggatg cgagtgcggg catcgtgccc aacgcgggca ccggctttaa accgccgctc
     2521 aatccggtga acccgccaac caccgacagc aagagcctca tcgttcgcaa gaacgccagc
     2581 ggccagtggc tcgacgacaa cggtggcgac tggacgaaca tggtgtcggg ctccgcggcc
     2641 ggcaa
//
LOCUS       DAOQOC010000341         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22164_length_2631_cov_3.505047, whole genome shotgun sequence.
ACCESSION   DAOQOC010000341 DAOQOC010000000
VERSION     DAOQOC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22164_length_2631_cov_3.505047"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PL106_06470"
     CDS             <1..592
                     /locus_tag="PL106_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA32748.1"
                     /translation="GFRVNTFTHVLEGYKVAELLAKHDANAATFSDWWAYKMEVMDAI
                     PYNAALLHDQGVNTCINSDDAEMGRRLNQEAAKTMKYGGVPAEDAWKMVTLNPAKALH
                     LDGRMGSVETGKDADIVLWNTDPLSIGAKAEMTFVDGVRRFDAKVDRELRAAIKAERE
                     RLISKMIAAKKAGAPVRKAEREGRGRWECGTIGEEP"
     gene            589..1953
                     /locus_tag="PL106_06475"
     CDS             589..1953
                     /locus_tag="PL106_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA32749.1"
                     /translation="MNMPASGKRQKAGSSGQPGTVTLGALLIACCLLSVAAQAQKPTP
                     APPETRSILLKGGTVHAGDGTVIDDAAVGFRNGTIDFVGHSYGTTAKYDTVINTDGQH
                     IYPGLILPDNTLGLHEIDLVNSTADEEETGDMEPEVRAISAYNTDSRIIPTVRANGVL
                     IAQVTPRGGTIAGSSTVVRLDAWDNAAAPIRAVDGIRMTWPRAYERTGWWAEPGDTRR
                     SSADERSKKIQQIEEFFRKAAAYAASREHPLVDQRMGSMRGLFNGSMTLYVEADGAQE
                     IQEAVLFAQAMGVQRTVIVGGYDAWRVADLLRDKKVDVILRRIHSLPLRDDDDTDLPY
                     RLPVLLRSKGVRFCLGYAGDQERPGGRNLAFTAGTAAAYGLEREAALQAITLDAARVL
                     GIDQRYGSLTTGKSATLFVSTGDALDMRTNNVVHAFIDGRHIVLDDHQKALYRQYRDR
                     YAKP"
     gene            2015..>2631
                     /locus_tag="PL106_06480"
     CDS             2015..>2631
                     /locus_tag="PL106_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNA32750.1"
                     /translation="MNELTTLLDLAIAAAFAAGRAIMEVYAGPVKVELKDDRSPLTEA
                     DRQAHEAILHELAVTRLPVLSEEGKKIPVAERQGWERYWLVDPLDGTKEFIKRNGEFT
                     VNIALMRAIKGEGLVAALGTHTPGASALYVPAKDILYFAWEGGGAFRVERAATRGTTA
                     TLKLAALAQRLPLPMPSERPFTIVSSRSHAGPDTSAFIARMRMKHG"
BASE COUNT          528 a          799 c          891 g          413 t
ORIGIN      
        1 gggcttccgg gtgaacacct tcacccatgt cctggaaggg tacaaggtgg cggagctgct
       61 cgcgaagcac gatgccaacg cggccacctt cagcgattgg tgggcctaca agatggaagt
      121 catggacgcc atcccgtaca acgccgcgtt gctccatgat cagggcgtga acacgtgcat
      181 caacagcgac gatgccgaga tgggccgcag gttgaaccag gaggccgcga agaccatgaa
      241 gtacggtggt gtcccggcgg aagatgcctg gaaaatggta acgttgaacc ccgcgaaggc
      301 ccttcacctg gacggccgca tgggctcggt ggaaacgggc aaggacgcgg acatcgtgct
      361 ttggaacacg gacccgctga gcatcggtgc caaggccgag atgaccttcg tggacggcgt
      421 gcggcgcttc gatgcgaaag tggaccggga actgcgagcg gccatcaagg cggaacgcga
      481 gcggctcatc agcaagatga tcgcggcgaa gaaggcggga gcgccggtcc ggaaggccga
      541 acgtgagggt cggggtcgtt gggaatgcgg aacgatcggg gaagagccat gaacatgcca
      601 gcaagcggca agcgacagaa ggcgggcagc agcggccaac caggtaccgt gaccctcggg
      661 gcgctgctca tcgcgtgctg cctgttgtcc gttgccgcac aggcccagaa gcccacgccc
      721 gcacctccgg agacaaggag catcctgctg aagggcggta cggtccatgc gggggatgga
      781 acggtcatag acgatgcggc ggtcggcttc cgcaacggga cgatcgactt cgtagggcac
      841 agttacggca ccacggcgaa atatgatacc gttatcaata ccgacggcca gcatatctac
      901 cccggactga tcctgccgga caacacgctc ggtctgcacg agatcgatct ggtgaactcc
      961 accgccgatg aggaggagac cggtgacatg gagcctgagg tgcgcgccat atcggcctac
     1021 aacaccgact cgcggatcat ccccacggtg cgggccaacg gcgttctgat cgcccaggtc
     1081 acgccgcggg gcggaaccat cgccggatcg agcacggtgg tgcggttgga tgcctgggac
     1141 aacgccgcgg ccccgatccg tgcggtggac ggcatccgca tgacctggcc ccgggcctac
     1201 gaacgcacgg gctggtgggc cgaaccaggg gatacccgtc gttccagcgc cgacgaacgc
     1261 tcaaaaaaaa tacaacagat agaggaattc ttccgcaagg ctgcggcata cgcggccagc
     1321 cgggagcatc ccctcgttga tcagcggatg ggatccatgc gcggactttt caacgggagc
     1381 atgacgctgt atgtggaggc cgatggggcg caggagatcc aggaggcggt cctgttcgca
     1441 caggcgatgg gtgtgcaacg caccgtgatc gtgggcggct acgatgcttg gcgggtggcg
     1501 gatctcctgc gggacaagaa ggtggatgtg atcctgcgcc gcattcacag cctgcccttg
     1561 cgcgacgatg acgacacgga cctgccctac aggctgcctg tcctgttaag gagcaaaggt
     1621 gtccggttct gcctgggtta tgcaggggac caggagcggc ccggtgggcg caacctcgcc
     1681 ttcacggccg gaaccgccgc cgcttatggg ttggagcgcg aagcggccct gcaagccatc
     1741 accctcgacg ccgctcgtgt gctgggtatc gaccagcgct acggcagcct gaccacaggc
     1801 aagagcgcca ccttgttcgt ttccaccggc gacgcgctgg acatgcgcac caacaacgtg
     1861 gtccacgcgt tcatcgacgg gcggcacatc gtgctggacg accatcaaaa agcgctctat
     1921 cggcagtacc gtgatcgcta tgccaaaccc tgatcgttga cagtcctccg cgtgcggctc
     1981 cgtcacaagc gatgacgggg atattttcgg cccggtgaac gaattgacca ccctgctgga
     2041 ccttgcgatc gccgcggcct tcgcggccgg tcgtgcgatc atggaggttt atgcgggtcc
     2101 cgtgaaggtg gaactgaagg acgaccggtc gccgttgacc gaggcggaca ggcaggcgca
     2161 cgaggccatc ctgcacgaac tcgcggttac gagactcccg gtgttgagcg aggaaggcaa
     2221 gaagatcccg gtcgccgaac gccagggctg ggaacgctac tggttggtgg acccgctgga
     2281 cggcacgaag gagttcatca agcgcaacgg cgagttcacc gtgaacatcg cgctgatgcg
     2341 cgcgatcaag ggcgagggcc tggtcgcggc cctgggtacg cacacgcccg gcgcatcagc
     2401 actgtatgtg cccgcgaagg acatcttgta cttcgcctgg gaaggtggcg gtgccttccg
     2461 tgtggagcgg gcggcaaccc ggggcaccac tgccacgttg aaactcgctg cgctggccca
     2521 gcgtctaccc ttgcccatgc cgtccgagcg ccccttcacc atcgtgtcca gccgcagcca
     2581 tgccggcccg gacaccagcg cgttcatcgc gcggatgagg atgaagcatg g
//
LOCUS       DAOQOC010000342         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22191_length_2629_cov_3.733100, whole genome shotgun sequence.
ACCESSION   DAOQOC010000342 DAOQOC010000000
VERSION     DAOQOC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22191_length_2629_cov_3.733100"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..499)
                     /locus_tag="PL106_06485"
     CDS             complement(59..499)
                     /locus_tag="PL106_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32751.1"
                     /translation="MKRCFTALIALTGAALPSASGQGHSALVQLQAHHSQADLEHMEE
                     HAHYRYVGELLFYSSSFLVEEGGVERAATEQEVLGIDLHAHDGLRMVDQRIAVHDPLI
                     DKHIILLSRNEFGALVLERLSPLDRDAFLANKRATMEQPSSKTQ"
     gene            699..1406
                     /locus_tag="PL106_06490"
     CDS             699..1406
                     /locus_tag="PL106_06490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNA32752.1"
                     /translation="MALPLFYCPGTLYGILHLPEEEAHHARSVLRLSPGARIGLLDGK
                     GTVAEAVISTMDKRQLQVAITSTSLRPRERKARIHLAVGLTKQMERFEWMLEKSTEIG
                     VDRITPLITDRTERTRLRKDRLERILVGAMKQSQRAWLPVLDEMMTINDLVDQGIPVQ
                     RLFGWCEGRPSALATCYRPGEDAVLLIGPEGDFTPEEATMLTTHGFGAVSLGKARLRT
                     ETAAISSCAYMNLAQQV"
     gene            1449..2129
                     /locus_tag="PL106_06495"
     CDS             1449..2129
                     /locus_tag="PL106_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNA32753.1"
                     /translation="MNSRIPALIAFCSWALAPLGTRAQGTFQFAVLKYNGGGDWYANP
                     TAVPNLVKFCNQELGTNINTEVPNVEVGSPDIFTYPWLDITGHGNIVLNGQEAQNLRN
                     YLIGGGFLHISDNYGLDKFIRPALKSVFPELEFVELPFAHPVYHQKFDFPRGLPKIHE
                     HDGLPPQGFGLLWEGRLVCFYDYECDLGDGWEDPGVHNDSEEIRRKALQMGANLVRYV
                     FSGGVQGQ"
     gene            complement(2178..>2629)
                     /locus_tag="PL106_06500"
     CDS             complement(2178..>2629)
                     /locus_tag="PL106_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNA32754.1"
                     /translation="FLLYKSVKEIHHKLEGEGAEAKPASASKGFAALVLQIVLVDAVF
                     SFDSIVTAIGLADHIEVMIIAVVMAMIVMFFFADAISAFIEKHPALKMLALSFLVMVG
                     FMLFFEGLHPIHHSEIPKGYAYVAMAFSFGVELLNMRASKHVVKLNK"
BASE COUNT          551 a          812 c          753 g          513 t
ORIGIN      
        1 gatcatggtc gtgacaacaa cgaggatacc ggtggtggat cgtgtaccca tggcacggtc
       61 actgcgtttt ggaggacggc tgttccatgg tagcccgctt attcgccagg aacgcatccc
      121 gatcgagcgg gctcaaccgc tccagcacca gggctccgaa ttcgttgcgc gagagcagga
      181 tgatgtgttt gtcgatgagc gggtcgtgta ccgcgatacg ctgatcgacc atgcgcagcc
      241 catcatgggc gtgcagatcg atcccgagta cctcttgctc ggtcgccgcc cgttccaccc
      301 cgccctcctc caccagaaaa gaggaggaat agaacagcaa ctccccaaca tacctgtaat
      361 gggcatgctc ctccatgtgc tccaggtccg cttgggaatg gtgggcttgc aactggacaa
      421 gggcggaatg cccctgccct gatgcagaag gcagcgctgc gcccgtgagc gcgatgaggg
      481 ccgtgaagca gcgtttcatc aacatgagta ttgggcaagt taagatgatc ccgagccgtt
      541 gtcaagctgg gacggttgcg atcggtgcct gccccgcatc tattgacgct ggatacgtcg
      601 gtcggatgcc tcgacggaac accatccggc agtgcgactt cccacgtacc agcattgtcc
      661 ggaccgtatg gaatcccaac ttcgccgccc gacgtaccat ggccctaccg ctcttttact
      721 gcccagggac actctacggc atactccacc tgccggaaga ggaggcccac cacgcgcgtt
      781 cggtccttcg cctctcccca ggtgcccgga tcggcctgct cgatggcaaa ggaaccgttg
      841 ctgaagccgt catctccact atggataagc gccagttgca ggtggccatc acttcgacga
      901 gcctgaggcc tcgggaacga aaagcacgga tccacctcgc cgtaggcttg accaaacaga
      961 tggaacgctt tgaatggatg ctggagaaaa gcaccgagat cggcgtggac cgcattacac
     1021 cactgatcac cgaccgtacg gaacggacgc gtttacgcaa ggatcgcttg gaacggatcc
     1081 tcgtcggtgc gatgaaacaa tcgcaacgtg cctggcttcc agtgttggat gagatgatga
     1141 cgatcaacga cctggtcgat caagggatcc ccgttcagcg attgttcgga tggtgcgagg
     1201 gtcggccatc ggcgctggcc acgtgctacc gaccgggcga ggacgccgtg ctgctcatcg
     1261 gtccggaggg cgacttcacg ccggaagaag cgacgatgct taccacgcac ggtttcggag
     1321 ccgtaagctt gggcaaggca cgactgcgca ctgagacggc cgccatcagc tcctgcgcgt
     1381 acatgaacct ggcacaacag gtgtgaacga tccatcgcac tcccgcgcag cccccccatc
     1441 ttcgccgaat gaattcccgg atccccgccc tgatcgcctt ctgttcatgg gcacttgcac
     1501 ctttgggcac tcgcgcgcaa ggcaccttcc agttcgccgt gctcaaatac aacgggggtg
     1561 gcgattggta tgccaatccg accgcagtgc ccaacctggt gaagttctgc aaccaggaac
     1621 ttggtaccaa catcaatacc gaagtaccca atgtggaggt gggcagccct gacatcttca
     1681 cctacccttg gctcgacatc accggtcacg gcaacatcgt tctcaacggg caggaagccc
     1741 agaacctcag gaactacctg atcggtggtg gtttcctgca catcagcgac aactacggac
     1801 tggacaagtt catccgccct gccttgaagt cggtgttccc cgagctggaa ttcgttgagc
     1861 tgcctttcgc acaccccgtt tatcaccaga agttcgactt cccacgtgga ctgcccaaga
     1921 tccatgaaca tgatggtctt cccccgcagg gcttcggcct cctgtgggaa ggacggctgg
     1981 tgtgcttcta cgactacgag tgcgacctgg gcgatgggtg ggaggatccc ggggtacaca
     2041 atgacagcga ggagatcagg cgcaaggccc tgcagatggg cgcgaatctg gttcggtatg
     2101 ttttctccgg cggggtacag ggccaatagc gcggtccgtc cggtgcggac gacccggcct
     2161 caacgtgatc gttcgcgtca cttgttcagc ttcaccacgt gtttgctggc ccgcatgttc
     2221 agcagctcca cgccgaagct gaaggccatg gccacgtacg cataaccttt cgggatctcg
     2281 ctgtggtgga tcgggtgcaa gccctcgaag aagagcatga agcccaccat caccaggaag
     2341 gagagcgcca gcatcttcag ggccggatgc ttctcgatga aggcgctgat cgcatcggcg
     2401 aagaagaaca tcacgatcat cgccatcacc accgcgatga tcatcacttc gatgtgatcg
     2461 gccaggccga tcgccgtgac gatgctatcg aagctgaaca ccgcatccac cagtacgatc
     2521 tgcaacacca atgcggcgaa tcccttgctg gctgaagccg gtttcgcctc ggcaccctcc
     2581 ccttccagct tgtggtggat ctccttcacc gacttgtaca acaggaaca
//
LOCUS       DAOQOC010000343         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22283_length_2623_cov_3.269470, whole genome shotgun sequence.
ACCESSION   DAOQOC010000343 DAOQOC010000000
VERSION     DAOQOC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22283_length_2623_cov_3.269470"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PL106_06505"
     CDS             complement(<1..325)
                     /locus_tag="PL106_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32755.1"
                     /translation="MSSERKDGKMSSLFVAIVIPLALLVAILFYMFILGDPANFEGGD
                     PIKGHPIEAEPGHTFGIIHKGGFIVPILITVNLLVIIFSIERFLTLSKAKGKGRGAGK
                     ARKAAK"
     gene            complement(669..1607)
                     /locus_tag="PL106_06510"
     CDS             complement(669..1607)
                     /locus_tag="PL106_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32756.1"
                     /translation="MRKQLLLPALVLSMSTTAQTTLLAESFDGYTEGSGMVTNDPANW
                     AIWPGGEDQVVSTAFAQSGTQSLGCISTNAANGGPGDLLLRLGDRTSGVYDLGWSMLV
                     PSGKGGYFNIQHAENVGSSGSFGLEVTFAGGTVTAVAAGQTLTTAYVPGEWTDVLINV
                     DLENDVAALFINGTSLITWEFRYNTANAEVTPQLGAIDFFSYGGGTDMGEAYVDDVSF
                     VQTSAGGIGISENMAGMLRVYPNPVQDVVTIELAEGSTAGASIRLVDASGRLVARPVI
                     LPMRRAQFDMSGLTAGVYSLRLDDGDQHRVVRVIKQ"
     gene            complement(1668..2441)
                     /locus_tag="PL106_06515"
     CDS             complement(1668..2441)
                     /locus_tag="PL106_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNA32757.1"
                     /translation="MANYDRDLKLAVLIDADNVPYGAISEMMEEVGKYGIATYKRIYG
                     DFTSNRLQGWKDVLNEHAITPMQQYSYTQGKNATDSALIIDAMDILYTGDVDGFFIVS
                     SDSDFTKLATRLREKGKRVYGMGEKKTPKPFITACEKFIYIEVLRQEPASTGSKVGSK
                     PGRGAKDAVSTEPDPTAALTDKSLRKLVLSTIEDFADEQGWLPMAALGNSILKKRPEF
                     DARNYGFKRLSDLVKALPYVDVEERQTGSGNKHDFVRWK"
BASE COUNT          483 a          808 c          775 g          557 t
ORIGIN      
        1 atttggcggc cttcctggct ttgcccgcgc cccggccctt gcccttggcc ttgctcaagg
       61 tgaggaatcg ttcgatgctg aagatgatga ccagcaggtt cacagtgatc aggatgggca
      121 cgatgaagcc gcccttgtgg atgataccga aggtgtggcc cggctcggcc tcaatgggat
      181 gaccctttat cggatcgcca ccctcgaagt tggccggatc gccgaggatg aacatgtaaa
      241 agaggatggc gaccagcagc gccaggggga tcacgatggc cacgaagagg ctggacatct
      301 tgccgtcttt gcgttcagag ctcatgaatg gaggggttgc gtttcgttga ttgttcagtc
      361 ggtgatgttc tcgtggtacc cgtgacccgc atagccggta tgctacggtc gcaagggggg
      421 caaacatagc aaagggccgg ttcggcccat gtgaaaggaa ccgtgccgcg ttgcttcacc
      481 cacccccgcg tcccctgact cctggagcgg gggcaagagg cctggttgcg ttgcgccgtg
      541 catcggggca tcaacgcggc gatgcgtgct gacgtgcgta caccatgctt gccgaacaag
      601 gaacggtccg tccggaacga ggaacgggga agttcgcacc gccccgttcc cgcttccgtc
      661 agatgctttc actgtttgat cacgcgcacc acgcgatgct ggtcgccgtc atccagtcgc
      721 aacgagtaca ccccggcggt caggccactc atatcgaact gcgccctgcg catcggcagg
      781 atcacgggcc gggcgaccaa gcggcccgac gcatccacga ggcggatgga cgcaccggca
      841 gtgcttcctt cggccaattc gatggtcacc acatcctgca cggggttggg atatacgcgc
      901 agcatcccgg ccatgttctc gctgataccg atgccgccag cactggtctg tacgaagctg
      961 acatcgtcca cgtacgcctc ccccatgtcg gttccgccac cgtaggagaa gaagtcgatg
     1021 gctccaagtt gaggtgtcac ttcggcgttg gccgtgttgt accggaactc ccaagtgatc
     1081 agggatgtgc cgttgatgaa gagcgccgcc acgtcgttct cgagatccac gttgatgagg
     1141 acgtccgtcc attcaccagg gacatacgcg gtggtcaacg tctggcccgc ggccacggcg
     1201 gtcacggttc ctcctgcgaa ggtgacctcc aatccgaacg agccactgct gccaacgttc
     1261 tcggcgtgct ggatgttgaa atagcctccc ttgcccgagg gaacgagcat ggaccagccc
     1321 agatcgtaca ccccactggt acgatcgccc agccgcagga gcagatcgcc cgggccacca
     1381 ttggccgcat tggtactgat gcaaccaagg ctttgggtac cggattgtgc gaaggccgtt
     1441 gagaccacct ggtcttcgcc accgggccag atagcccagt tcgcgggatc gttcgtgacc
     1501 attccgctgc cttccgtgta tccatcaaac gattcggcaa gcaaggtggt ctgcgccgtg
     1561 gtcgacattg agaggaccaa ggcaggaagg agtagttgtt ttctcatcgc gcttcgggtt
     1621 tgggaatgcg atgtgaatat aaccgttggt cccatacgca tatcacctta cttccagcgg
     1681 acgaagtcat gcttgttgcc cgatccggtc tgtcgctcct ccacatccac gtatggcagt
     1741 gccttcacca gatcgctcag ccgcttgaag ccgtagttcc gcgcatcgaa ctccggacgc
     1801 tttttcagga tcgaattgcc cagcgccgcc atcggcagcc aaccctgctc gtccgcgaag
     1861 tcctcaatag tgctcagcac cagtttgcgc agcgacttgt ccgtgagggc cgctgtggga
     1921 tcgggttccg tgctcaccgc atccttggct cccctccccg gctttgagcc gactttcgag
     1981 ccggtgctgg ccggttcctg acgtagcact tcgatgtaga tgaacttctc acaagcggtg
     2041 atgaagggct tcggcgtctt cttctcgccc atgccgtaca cacgcttgcc cttctcgcgc
     2101 agtcgcgtgg ccagcttcgt gaagtcacta tcgctgctca cgatgaagaa gccgtccaca
     2161 tcccccgtgt acaggatgtc catcgcatcg atgatcagcg cgctgtcggt ggcgttcttg
     2221 ccctgggtgt agctgtactg ctgcatgggc gtgatggcat gctcgttcag cacatccttc
     2281 cagccttgca ggcgattgct cgtgaaatcg ccatagatcc gcttgtaggt ggctatgccg
     2341 tacttgccca cctcctccat catttcgctg atcgcgccgt agggcacgtt gtccgcgtcg
     2401 atgagcacgg ccagtttcag gtcacggtcg tagttcgcca tgcgtgtaaa gcctgcgttc
     2461 ggggtgaagg cgctggaaag ctaaccggct gcgcctttgg gcaccccgtt atcttcgcgg
     2521 ccctttcaaa agtcccgatg gacaagcgtt tcccgcaata cgacgaactc gacctgccca
     2581 aggtcgccaa ggaggtcctg cagcaatggg aggccgagga cac
//
LOCUS       DAOQOC010000344         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22430_length_2614_cov_3.301680, whole genome shotgun sequence.
ACCESSION   DAOQOC010000344 DAOQOC010000000
VERSION     DAOQOC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22430_length_2614_cov_3.301680"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PL106_06520"
     CDS             <1..764
                     /locus_tag="PL106_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32758.1"
                     /translation="TASLAVQNPDADPVNNTAMVNTTVTASLDPNDKLARTSSQGSSE
                     NYYLDLDEWIDYTIRFQNTGTDTAFNVVIIDSLPPTLDPTTLLVGATSHPMSWSISGP
                     GVLRFHFPDILLPDSTTDEPKSHGLVTFRIMPQVLTPGTVIENEAGIYFDFNDPVITE
                     PSVLVAEFSTGVEEHGAEVMIIFPNPTMGIITLTGAELIDARVLSLDGKLLYTARSAT
                     KPIATIDLSGLSPGTYLLEGKSADGNRRTVRFTKL"
     gene            769..>2614
                     /locus_tag="PL106_06525"
     CDS             769..>2614
                     /locus_tag="PL106_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32759.1"
                     /translation="MRPTLCVFLLLASAGSSRAITIVLVGAMDPSCFSATGVIDVFVT
                     GGTPPYSYQWSNGATTEDLIGVVAGYYELTVTDAVSAQATDGWTLVSVPLSSAASAQD
                     GHASCVGSMSGRVQVIEWGINGTPPYSYSPPPDGFDPQGDPYFEFFGTPPGSEVQIQV
                     TDALGCTGVLTEYIIAPQLSGGPQMTVTGIQGSCSNGSGGAAMVGNVYDGTFFGGPDY
                     VLLDEAQFAVASGFNAGNTISFTGLAPGDYQFVRDWDPSGVYMAYSCNGIFFDQVGFT
                     IPDLGPVCGAVSGSVFIDHDADCTQDAVDIPVPFQVLEILPGPVHTITNSAGVFSIDL
                     PDGSYILGQTDPTLVQLCPVSQPVPFTIASNQIEVHLADSSTQPLDLMVDLDPGPMRP
                     GFDGAYWGEVRNLSLQLTGTVSVTLTLDPVLDFLGAAPAPTSVTGNVITWDLTEIAPL
                     SDFDLSVQVHVPTTTPIGTPLSTTLSATCSVAETDISNNTVAVVQQVTGSYDPNDKTA
                     RTSSGSSDDLYYIDQDEWIDYVIRFQNTGTASAINVVITDTLPVELDMATFEQGVASH
                     PFDIRFKADRVVEWSFENINLPDSGANEAASHGLVSFRIRPQLPLLAGT"
BASE COUNT          498 a          855 c          765 g          496 t
ORIGIN      
        1 caacggcctc gctcgccgtg cagaatcccg atgccgatcc ggtcaacaac accgccatgg
       61 tgaacaccac cgttaccgcc agcctcgatc ccaacgacaa gctggcgcgc accagctcgc
      121 aagggagcag tgagaactac tacctcgatc ttgatgaatg gatcgactac accatccgct
      181 tccagaacac gggcacggac acggccttca acgtggtgat catcgactcg ctgccgccca
      241 cgctggatcc gaccaccctg ctggtgggcg ctacttcgca ccccatgagt tggagcatca
      301 gtggtccagg cgtcctgcgg ttccacttcc cggacatcct gttacccgac agtaccaccg
      361 atgagccgaa gagccacggc ttggtcacct tccgtatcat gccccaggtg ctcacaccag
      421 gcacagtgat cgagaatgag gcaggcatct acttcgactt caatgatccg gtgatcaccg
      481 agccgagcgt gctcgttgct gagttcagca cgggggtgga ggagcacgga gccgaggtga
      541 tgatcatctt cccgaatccc acgatgggga tcattacgct cacgggcgcg gaactcatcg
      601 atgcgcgggt gctttcgctg gacgggaagc tgctttacac cgcgcgctcg gcaacaaagc
      661 ctattgccac catcgacctc agcggccttt cacccggcac ctatttgctg gaaggcaaaa
      721 gcgccgatgg gaaccggcgt accgttcgat tcaccaaact ttagcaccgt gcgacccaca
      781 ctttgcgtct tcctactact ggcatcggca ggctcatcgc gcgccatcac cattgtcctc
      841 gtaggtgcca tggatccgtc ctgcttttcg gctacagggg tcattgacgt tttcgttacc
      901 ggcggcacgc cgccctacag ctaccaatgg agcaacgggg ccaccacgga ggatctcatc
      961 ggggtggtcg caggctatta tgagctgact gtgaccgacg ccgtgagtgc gcaagccacc
     1021 gacgggtgga cgctcgtatc cgtgccgctc tcctcggccg ccagcgcgca agatggtcat
     1081 gccagttgcg tgggttccat gagcggccgt gtgcaagtga tcgaatgggg catcaacggc
     1141 acgccgccgt attcctactc gccgccgccg gatggcttcg atccgcaagg cgatccctac
     1201 ttcgagttct tcggcacgcc gccggggtcc gaggtgcaga tccaggtgac ggacgcgttg
     1261 ggatgcaccg gcgtgctcac cgagtacatc atcgcaccgc agctctccgg cgggccgcag
     1321 atgaccgtga ccgggatcca aggctcatgc tccaacggct ctggcggcgc agccatggtt
     1381 ggcaacgtgt acgatggcac cttcttcggc gggccggact acgtgctcct cgatgaagcg
     1441 cagttcgcgg tggcaagcgg tttcaacgcg gggaacacca tctcattcac cggactggcg
     1501 cccggcgact accagttcgt gcgtgattgg gaccccagcg gggtctatat ggcctacagt
     1561 tgcaatggca ttttcttcga ccaggtgggc ttcaccatcc cggacctcgg tccggtatgc
     1621 ggcgctgtga gcggcagcgt tttcatcgat catgacgcgg actgcacgca ggatgccgtg
     1681 gacattcccg tgccgttcca ggtcttggag atccttcccg gcccggtaca caccatcacc
     1741 aattccgctg gcgttttctc catcgacctg cccgatggga gctacatcct ggggcagacc
     1801 gaccccacgc ttgttcagct ctgccccgtg agccagcccg ttcccttcac gatcgcatcg
     1861 aatcagatcg aagtacacct cgccgacagc agcacgcagc cgctggacct catggtcgat
     1921 ctcgatcccg gcccgatgcg tccgggcttc gatggcgcgt attggggtga ggtccgcaac
     1981 ctgtctctcc aactcacagg cacggtcagc gtcaccctca cgctggatcc cgtgctcgat
     2041 ttcctgggcg cagcgcccgc gccaacatcg gtgaccggta acgtgattac ctgggacctg
     2101 accgagatcg cgccgctcag cgacttcgat ctcagcgtgc aggtgcatgt gccgacgacc
     2161 acgcccatag gcacgccgct gagcaccacg ctgagtgcga cgtgctcggt ggcggagacg
     2221 gacatctcga acaacacggt cgcggtggtg cagcaggtca cgggcagcta cgaccccaac
     2281 gacaagacgg cgcgcacgag cagcggttcg agcgatgatc tctactacat cgaccaggat
     2341 gagtggatcg actacgtgat ccgctttcag aacactggga ccgccagcgc gatcaatgtg
     2401 gtgatcaccg atacgctgcc ggtggaattg gacatggcca ccttcgagca gggcgtggcc
     2461 tcgcatccct ttgatatccg cttcaaggca gatcgcgtgg tggaatggag cttcgagaac
     2521 atcaacctgc cggacagcgg ggccaatgaa gcggcaagcc acggcctcgt cagtttccgt
     2581 atccggccgc aactgccctt gcttgccgga acgg
//
LOCUS       DAOQOC010000345         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22448_length_2613_cov_3.117279, whole genome shotgun sequence.
ACCESSION   DAOQOC010000345 DAOQOC010000000
VERSION     DAOQOC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22448_length_2613_cov_3.117279"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2276)
                     /locus_tag="PL106_06530"
     CDS             complement(24..2276)
                     /locus_tag="PL106_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA32760.1"
                     /translation="MKHLIHFRALPLLAVPWLIGTGNAAGQNVSVLSGTVQGRDAFGK
                     REPVPFAFVAAMGQAASTSADQLGHFKLEAPLSFPLRLVASAVGYANDTLFLEQPSTG
                     ITLTLPEVIELRATEVIERQAGTRMDLRATQGNERITAKELKRAACCDVSESFESNAT
                     VDVSYSDAVSGAKRISMLGLDGRYVQMGMENIPFVRGLSSAYGFTLVPGPWIREINVS
                     KGIGTAVNGPNAMTGQIDLCLQDPYEAPPLFTNLYANNQGRTELNVNTGQHWGKAGAN
                     LLMVQGSMNQRDMDDNGDGFRDQPLTRRFNIMDRWLQQKGRRTTQLIARYVTDARDGG
                     HTDAHRPGEGPAGRHYTVHVANEMADVLAKNGWILRDSTKSIGILASFRNHTLSSNYG
                     DRTYEGAQLSGYGSVVYQQLIGARGDQMKVGAAFQYDAYHERFIDSTFARTERMPGLF
                     AEYTRSREKLTIVAGLRADHNSVFGTAVSPRLHAKYAISPLTALRLSAGHAFRTALPF
                     VENAGVLASSRNIVVEGPLGMERSWNFGISLLHKWKWLGRKWELGIDAYRSEFTQQVV
                     ADLDRSPRTMAIYMLHGPSFANSVLADVQVELSSTLRAKASYRWYDVRATYDGVLRER
                     PFTPRHRGLVDLAYESRNERWRFDIFTNLFGTSRLPRTAANPKEYRLPERAPAYATLN
                     AQVTWALGALEVYIGGENLTSATQHRQILAPEDPFGPYFDASMIWGPTQGAMVYGGFR
                     HTLKRKDPKP"
     gene            complement(2355..>2613)
                     /locus_tag="PL106_06535"
     CDS             complement(2355..>2613)
                     /locus_tag="PL106_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32761.1"
                     /translation="GDPEHHDGPTFRSACCTVDRTAPEHHAFTTVDAPIHLIAPEAPG
                     AMTFGVNPRVAVEPCGERRPSTRPPPLLTAERLSLERSYLI"
BASE COUNT          442 a          785 c          893 g          493 t
ORIGIN      
        1 aaggaggagg aggaggaaac gtttcatggt tttgggtctt tgcgtttgag ggtgtgccgg
       61 aagccgccgt agaccatggc gccctgcgtg ggtccccaga tcatggaggc atcaaaataa
      121 gggccaaagg gatcctccgg agcaaggatc tgccggtgct gcgtcgcgga ggtgaggttc
      181 tccccgccga tatagacctc cagggcaccg agggcccagg tcacctgggc gttcaaggtg
      241 gcgtaggccg gtgcgcgttc cggaagtcgg tactccttgg ggttggcggc ggtgcggggc
      301 agcctcgagg tgccgaacag gttggtgaag atgtcgaagc gccaacgttc gttgcggctc
      361 tcgtaggcca agtccacaag tccgcggtgc cggggcgtga agggccgctc gcgcaagacc
      421 ccgtcgtagg tggcacgcac atcataccag cggtagctgg ccttggcacg cagcgtgctc
      481 gacagttcaa cctgcacatc ggccagcacg ctgttggcga agctgggccc atgcagcatg
      541 tagatggcca ttgtgcgcgg gctgcggtcc agatcggcca ccacctgctg ggtgaactca
      601 ctgcggtaag catcgatacc gagttcccat ttgcggccga gccacttcca cttgtgcagc
      661 agcgaaatgc cgaaattcca ggagcgttcc atacccagcg ggccctccac aacgatgttg
      721 cgcgagctgg ccagcacgcc cgcgttctcg acgaagggca atgcggtgcg gaaggcgtgg
      781 ccggccgaga ggcgcagagc ggtgagcggg ctgatcgcgt acttggcatg gagcctgggc
      841 gaaacggccg ttccgaacac gctgttgtga tcggcgcgca gccccgccac gatcgtgagc
      901 ttttcccgcg agcgggtgta ctcggcgaag agccccggca tgcgctcggt acgggcgaag
      961 gtcgaatcga tgaagcgctc gtggtacgcg tcgtactgga aggccgcacc gaccttcatc
     1021 tggtcgccgc gcgcgccgat gagctgctgg tacaccacgc tgccgtagcc gctcagctgc
     1081 gctccctcgt acgtccggtc gccataattg ctggaaaggg tatgattcct gaacgaggcg
     1141 aggatgccga tgctcttggt gctgtcgcgc aggatccagc cgttcttggc gagcacatcg
     1201 gccatttcgt tggccacatg cacggtgtaa tgccgaccag cggggccttc gccgggccgg
     1261 tgcgcatcgg tgtggccgcc atcgcgcgca tcggtcacgt aacgggcgat cagctgtgtg
     1321 gtgcggcgcc ccttctgctg cagccatcgg tccatgatgt tgaagcggcg tgtgagcggc
     1381 tggtcgcgga agccgtcgcc gttgtcgtcc atgtcgcgct ggttcatgct gccttgcacc
     1441 atgaggaggt tggcgcccgc cttgccccag tgctgtcccg tgttcacgtt cagctccgta
     1501 cgcccctgat tgttggcgta caggttggtg aacaggggcg gcgcctcgta cgggtcctgc
     1561 aagcagaggt cgatctggcc ggtcatggcg ttggggccgt tcaccgctgt gccgatcccc
     1621 ttgctcacgt tgatctcccg gatccaggga ccggggacca gggtgaaacc gtaggcgctg
     1681 ctgagtccac ggacgaacgg gatgttctcc atgcccatct gcacatagcg gccatcgagc
     1741 ccgagcatgc tgatccgttt ggccccgctg accgcatcgc tgtagctcac gtccaccgtg
     1801 gcattgctct cgaagctctc gctcacgtcg cagcacgccg cgcgcttcaa ttccttggcg
     1861 gtgatgcgtt cgttgccctg ggtggcgcgc aggtccatgc gggtcccggc ttgtcgttcg
     1921 atcacttcgg tggcacgcag ctcgataacc tcagggagcg tcaacgtgat acccgttgac
     1981 ggttgttcca gaaataaggt atcgttcgcg taaccgacgg ccgaagccac cagccggagc
     2041 gggaatgaga gcggtgcctc cagcttgaaa tggcccagct gatcggcgga tgtggacgcg
     2101 gcctgcccca tagcggcaac gaaggcgaag ggcacgggtt cgcgcttgcc gaaggcatca
     2161 cggccctgca ccgtgccgct cagcacggat acgttctggc ctgcggcgtt acctgtgccg
     2221 atcagccagg gcacggccag taacggcagt gcgcggaaat ggatgaggtg tttcaatggg
     2281 aatgatcttt ccgttggaca aagaggaaca gccgcatcct ccggccggag ggtgcggaac
     2341 gggaaagacc gttctcagat caggtaactg cgctccaagc tcagtcgctc ggccgtcagt
     2401 aacgggggcg ggcgtgtgga ggggcggcgc tcaccgcatg gttccaccgc aacacgaggg
     2461 ttcacgccga aggtcatcgc tccgggggcc tctggtgcga tcaggtggat cggtgcgtcc
     2521 accgtggtga acgcatgatg ttccggagcg gtccggtcaa cggtgcagca cgcactcctg
     2581 aaggtggggc catcgtgatg ctccgggtcc ccg
//
LOCUS       DAOQOC010000346         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22685_length_2599_cov_3.983491, whole genome shotgun sequence.
ACCESSION   DAOQOC010000346 DAOQOC010000000
VERSION     DAOQOC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22685_length_2599_cov_3.983491"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PL106_06540"
     CDS             <1..780
                     /locus_tag="PL106_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA32762.1"
                     /translation="LHPPDEKNLCTWAMRCLLVETDGHLILIDNGIGDKQDAKFFGHY
                     DLHGDDTLDKSLAKHGFSKDDITDVFLTHLHFDHCGGSIVRQGNGYAPAFKNATYWSN
                     TYHWEWATKPNAREKASFLKENILPIQESGQLKFVDVERQQPTDTPYVKELFPDMDVL
                     LVNGHTDAMMIPHIRYKGRTLVYMADLLPSVHHVPLPWVMAYDTRPLLTMQEKSAFLE
                     RAADAHFTLFLEHDSVNQCATVERTEKGVRLASTHLLADLG"
     gene            836..1627
                     /locus_tag="PL106_06545"
     CDS             836..1627
                     /locus_tag="PL106_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32763.1"
                     /translation="MDTRSEIDITFDFRSDTPGYYTSNTDPDALSPTLRRYHKCLWSK
                     PLPSGDFFELVDTTPGVYLHHRSHLGEFRLTSDTVIPSFRKEARIAPFIAQEPDELAK
                     FNALGYTIGGMMLFPGNGIGNKMTINGSRGCHGKIKDRFDLTVECIRRHYRGDQSPLS
                     DVLDRYADFFDLFGDFHGYVEFFLLQDLLTEDRNTVRFMAPFEDFRTSPLPASFEAYS
                     TYRKRATEFIEARNRRILHFALGTAANTPQVGVVRRNDNETAEEQ"
     gene            complement(1728..>2599)
                     /locus_tag="PL106_06550"
     CDS             complement(1728..>2599)
                     /locus_tag="PL106_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNA32764.1"
                     /translation="NAITPKTKAVLLVHMCGAAADLDGIMAVCKEKNVMLIEDTAQAL
                     GATYKGKHLGLYGEMGSFSFDFFKINTCGEGGVVITNNHQRYTTAQHFSDHGHSHEGD
                     NRGMEPHYILGTNYRIGEINAAIGLAQARKLPFIIAQQRKHKAIIQARLARIPGLAFR
                     KQCDDAGDSATFFHLLFPSEAIARAAHKAMGEAGVGGAYWYDNMYHYIKQWDQLKELK
                     MPFRTVAHELGLPQDLKTIRFAKSDAVLSRLISFNIRVTWTEAELDAYLNKVEGAVRT
                     AFAAPSTEPAMTK"
BASE COUNT          545 a          759 c          757 g          538 t
ORIGIN      
        1 ctgcatccgc cggacgagaa gaacctgtgt acctgggcca tgcgctgcct gctggtggag
       61 accgatggcc atctgatcct gatcgacaac ggcatcggcg acaagcagga cgccaagttc
      121 ttcgggcatt acgacctgca cggcgacgac acgctggaca agtcgctggc gaagcacggt
      181 ttctcgaagg acgacatcac cgatgtgttc ctgacgcacc tccatttcga tcattgcggc
      241 ggcagcatcg tgcggcaggg caatggctat gcaccggcct tcaagaacgc cacctactgg
      301 agcaacacgt accattggga gtgggccacg aagccgaacg cgcgagagaa ggccagcttc
      361 ctcaaggaga acatcctgcc catccaggag agcggacaac tgaagttcgt ggatgtggag
      421 cgacagcagc caacggacac tccgtacgtg aaggagctct tccccgacat ggatgtgctg
      481 ctcgtgaacg gccataccga tgcgatgatg atcccgcaca tccggtacaa gggccgcacg
      541 ctcgtgtaca tggccgatct gctccccagc gtacaccacg ttccgctgcc ctgggtgatg
      601 gcctacgaca cgcggcccct gctcaccatg caggagaaga gcgccttcct ggaaagggcc
      661 gccgatgcgc acttcacact gttcctggaa cacgacagtg tgaaccaatg tgccacagtg
      721 gagcgcacgg aaaaaggtgt gcggctggct tcaacccacc tcttggctga tctgggatga
      781 acaccttggt cctgcccgag cagcgttgtt gcgtccggta agagaagctg gttacatgga
      841 cacgcgaagt gagatcgata tcacattcga cttcaggtcg gacacaccgg gttactatac
      901 ctcaaatacc gacccggacg ccctgagccc aacgctccgg cgataccaca agtgcctttg
      961 gagcaagcca ttgcccagcg gtgatttctt cgaactcgta gacacaacgc caggagtcta
     1021 tcttcatcat cggtcacatt tgggtgaatt ccgtttgacc agtgataccg ttattccctc
     1081 attccggaag gaggcgagaa tcgctccttt cattgcacaa gagcccgacg aattggccaa
     1141 attcaacgca ctcggttaca cgatcggtgg aatgatgctg tttccgggca acggaatcgg
     1201 aaacaaaatg accatcaatg gatcacgcgg atgccacgga aagatcaagg acaggttcga
     1261 tctcacggtc gagtgtattc gaaggcacta tcggggggat caaagtccat tgagcgacgt
     1321 actcgatcga tatgcggatt tcttcgacct ctttggtgac tttcatggat atgtggagtt
     1381 cttcctgctc caggaccttc tcacagagga tcggaacacg gtaagattca tggcaccatt
     1441 cgaagacttc cggacatccc cactacccgc cagtttcgag gcttactcta cgtatcgaaa
     1501 acgcgcgacc gagttcatcg aagcccggaa ccgccggatc ctacatttcg cgttggggac
     1561 agcagcgaat accccgcagg tcggcgttgt gaggagaaac gacaacgaaa cggccgagga
     1621 gcagtagtaa aggctcgacg ccgaggcgca tccggaggcg ctggaacgcc caaagaacct
     1681 acgcagacat tccttccatg accttgtttc actcgctgtg gcctttctta cttcgtcatg
     1741 gccggttctg tcgacggcgc ggcaaaggcg gtcctcaccg ctccctccac cttgttcaag
     1801 taggcatcga gttcggcctc ggtccaggtg acgcgtatgt tgaagctgat caggcgcgag
     1861 aggacggcgt cgctcttcgc gaagcggatg gtcttgagat cctgtggaag gccgagttca
     1921 tgggccacgg tgcggaaggg catcttcagc tccttcagtt ggtcccactg tttgatgtag
     1981 tggtacatgt tgtcgtacca gtacgccccg cccacaccgg cctcgcccat ggccttgtgc
     2041 gccgctcggg cgatggcctc gctcggaaag agcaggtgga agaaggtggc actgtcgccc
     2101 gcatcgtcgc attgcttgcg gaaggccagt ccggggatgc gtgcaagacg cgcctggatg
     2161 atggccttgt gcttgcgctg ctgcgcgatg atgaacggta gcttgcgcgc ctgtgccagg
     2221 ccgatcgctg cattgatctc gccgatccgg tagttggtac ccaggatgta atgcggttcc
     2281 atgccccggt tgtccccttc gtggctgtgg ccgtggtcgc tgaagtgctg ggcggtggtg
     2341 tacctctggt ggttgttggt gatcaccacg ccaccctcgc cacaggtgtt gatcttgaaa
     2401 aaatcgaagc tgaagctgcc catctcgccg taaaggccca ggtgcttgcc cttgtacgtg
     2461 gcacccaatg cctgggccgt gtcctcgatg agcatcacgt tcttctcctt gcacacggcc
     2521 atgatcccgt ccagatcggc agcggcgccg cacatgtgga caagcagcac ggccttggtt
     2581 ttcggcgtga tggcgttgc
//
LOCUS       DAOQOC010000347         2591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22803_length_2591_cov_3.140773, whole genome shotgun sequence.
ACCESSION   DAOQOC010000347 DAOQOC010000000
VERSION     DAOQOC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22803_length_2591_cov_3.140773"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PL106_06555"
     CDS             complement(<1..319)
                     /locus_tag="PL106_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA32765.1"
                     /translation="MRSRIARYIRQEKLLEHGQPVRVAVSGGVDSMVLVHVLRDLGHP
                     CSVVHVDHGLRGSESDTDAAFVQAYCHREKIPFVSTRVDVESHAKGTGISTQMAAREL
                     RYEL"
     gene            complement(337..1533)
                     /locus_tag="PL106_06560"
     CDS             complement(337..1533)
                     /locus_tag="PL106_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNA32766.1"
                     /translation="MPSPAPALNWRALATFPELLVRRTCAKYPGPGGWVVMARRSTGP
                     YVPEPLDQGTWAGRLVFDHPGVLDRPVGGLTSSGLHSAWWRPDVVLQWDGVRPGRGAE
                     SAEAPLLDALLATAEKPVTHRPIALWERRTPKDRYLATVRKLLAHIQRGDIYEINYCT
                     ERTTQLEDFDPYAAFARLLDATDAPFASLYRSGDLFALCMSPERFLRIEGRQVLTEPM
                     KGTRRRSRDAAEDAVLAHELAQDPKERSENIMAVDVARHDLSRIAAPGSVQGPELCAV
                     RTYPNVHQLVSTVRAELREGLGPWDAVRAAFPMASMTGAPKRRALELITEAEEMPRGL
                     FSGCLGYQLPDGTLDLNVVIRTLTWDARTGRASLITGGAITAQSDAEQEWEECELKAR
                     SILDAL"
     gene            1942..>2591
                     /locus_tag="PL106_06565"
     CDS             1942..>2591
                     /locus_tag="PL106_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32767.1"
                     /translation="MKKTATLFIAGIVSCALFAQNARLVFAGTAPNEAYVTFNPNPGA
                     VTTYMVIDNDAANAITQLAAGNGNIKSEHQNNRIRWRHQVGPLAVNYVIPWTTASNVK
                     IPLTISKTAGTATHDSASFVLATFNHQVWAGSNANTMWQNVNYMPTGVTHMNDFYLGS
                     VDNSENAVNRFWIIDPKHPGYAYTTAPNATMTFTNNDLDIDVGGAPGVNDDVLTTAS"
BASE COUNT          506 a          806 c          804 g          475 t
ORIGIN      
        1 gcagctcgta gcgcagctcg cgcgcggcca tctgggtcga gatccctgtc cccttcgcgt
       61 ggctctccac atccacccgg gtggaaacga acggtatttt ttcccgatgg cagtaagcct
      121 gcacgaaggc cgcgtcggta tcgctctccg atccgcgcag gccgtgatcc acatgcacca
      181 ccgagcaggg atggcccaag tcgcgcagca cgtgtacgag caccatgctg tccacgccgc
      241 cgctcacggc cacacgcacc ggctgtccgt gttccagcag tttttcctgg cggatgtagc
      301 gggcaatgcg gctgcgcatc ggtcaaagat gcacggtcaa agggcgtcga ggatgctccg
      361 tgccttcaac tcgcattcct cccattcctg ttcggcgtcg ctttgcgcgg tgatggcccc
      421 gcccgtgatc agggaagccc tgccggtacg ggcatcccac gtgagggtgc ggatcaccac
      481 gttcaggtcc aacgtaccat cgggcaattg gtaaccgagg cagccgctga acaacccgcg
      541 tggcatttct tccgcttcgg tgatcaactc cagcgcgcgt cgtttgggcg caccggtcat
      601 gctggccatg gggaaggcgg cgcgtaccgc atcccaaggc ccaaggcctt cgcgcaattc
      661 agcacgtacc gtactcacca gttgatgcac gttggggtag gtgcgtaccg cgcagagttc
      721 cggtccctgc accgagccgg gtgcggctat gcgggagagg tcgtgccggg ccacatccac
      781 cgccatgatg ttctcgctgc gctctttggg gtcctgcgcc agttcgtgcg cgagtaccgc
      841 gtcctccgcg gcatcacggg aacggcggcg tgtgcccttc atcggctcgg tgagcacctg
      901 tcgtccttca atgcgcagga aacgttccgg gctcatgcac agggcgaaaa gatccccgct
      961 ccggtaaagc gatgcgaacg gtgcgtcggt agcgtcgagc aggcgggcga acgcggcata
     1021 cggatcgaag tcctccagtt gtgtcgttcg ctcggtgcaa tagttgatct cgtaaatgtc
     1081 gccacgctgg atgtgtgcca gcagtttccg gaccgtggcc aggtagcggt cctttggcgt
     1141 gcgccgctcc caaagggcga tcgggcgatg ggtcaccggc ttttccgccg tggcgagcag
     1201 ggcatccagg agaggcgcct cggcggactc cgctccccgg ccgggccgaa cgccgtccca
     1261 ctgcaggacc acatcgggtc gccaccaagc ggagtgcagg ccggaggagg tgaggccgcc
     1321 cacggggcgg tccagcacac ccgggtggtc gaagaccagt cgcccggccc aggtgccttg
     1381 gtccaagggt tccggtacat aaggaccggt ggacctccgc gccatcacta cccagccgcc
     1441 cggtccggga tacttcgcgc atgtgcgacg aacgagcagt tcgggaaagg tggcgagggc
     1501 acgccaattg agggctgggg cgggagaggg cacggtcgca aaaattgcgt ggacggtgga
     1561 aactgcaccg agttgttcaa tagacatccg tcccaattgc ccagaggaat gaaaaagacc
     1621 tgtcacggtg agcctgccga accggtcctt gcttcgccgt agcggctacg caaaggcgcg
     1681 gtcgacaggc tcaccgtgac atgcaatagg aattcagacg gaggtcgaat tggagaatga
     1741 tccggttatg aaccgggggc aaccactcca gcgaagtcac gtctcttggt tgcatcggct
     1801 tggaggttcc atccgggtcc gatggatgca acggatccga accgcctgat tctcagcgac
     1861 ccgatgcttt cccgttcccg caagaaaaag atgctcgggg cttgccgccg gttcggcact
     1921 tcgcctaaat ttgacgctgc catgaaaaaa accgctacgc ttttcattgc tggtatcgtg
     1981 tcgtgtgcgc tgttcgcgca aaatgcgcgc ttggtgttcg caggcactgc cccgaacgag
     2041 gcgtacgtca cattcaaccc caaccctggg gccgtcacca catacatggt gatcgataat
     2101 gatgcggcga acgcgatcac ccaactggcg gcgggtaacg ggaacatcaa gagcgagcac
     2161 cagaacaacc ggatccggtg gcgccatcag gtaggtccgt tggccgtgaa ttatgtgatc
     2221 ccgtggacca cggcgagcaa cgtgaagatc cccctcacca tcagcaagac cgcaggcacc
     2281 gccacccacg acagcgcctc tttcgtgttg gccaccttca accaccaggt atgggcgggc
     2341 agcaacgcga acaccatgtg gcagaacgtg aactatatgc ccaccggtgt tacgcacatg
     2401 aacgattttt acttgggatc ggtggacaac tccgagaacg cggtgaaccg attctggatc
     2461 attgatccga agcatcctgg ctatgcgtat accaccgccc cgaacgcgac catgaccttc
     2521 acgaacaacg atctggatat cgatgtgggg ggcgcacccg gagtgaatga cgatgtgctg
     2581 acgaccgctt c
//
LOCUS       DAOQOC010000348         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22916_length_2584_cov_2.793990, whole genome shotgun sequence.
ACCESSION   DAOQOC010000348 DAOQOC010000000
VERSION     DAOQOC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22916_length_2584_cov_2.793990"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2584)
                     /locus_tag="PL106_06570"
     CDS             complement(<1..>2584)
                     /locus_tag="PL106_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNA32768.1"
                     /translation="NANGVSLGGDQTINTNLTLTNGKLTLGANNLTLALAATITNQSA
                     ARYLVTDGMGSLRRAINGSNVLFPIGTATSYLPATLSLTSGPQETFAARVQNEVYSEY
                     GAPGVPTGDLVVNEQVERTWTITEQTSGGNQATVQLQWNTVDEGANFGRSNCALHAYN
                     GTDWVAGALGGASGSNPYTRSLAGVSSFREFTVADGESNLPFACPGGVTPGSPCDDGS
                     ACTTNDVIGVDCQCAGIFQDSDNDGTCNADDGCPNDVNKIAPGECGCGVADVPASYYV
                     DADGDGYGTGALVPGFFTCTVPAGYSATAGDCDDNNNAMHPGAIEICDGFDNDCNGTV
                     DDPTTFSNGLVAQWLFNGNLNDHSTNGNNGTAPGGASFTTGMDGTANGAFQFGGSRYV
                     LVPHSNSLNLGTGDYTYSVWLNWAAGSLGNIFDKGESCCSPYRGLQCFVDHPSAGVFH
                     GRNNSSKAVSSSGGLGNNAWHHIVHTRTGSTMRIYVDGVLNATATHPVENITTSIALF
                     IGRNSASAIQFYSGKMDNLRLYNRALTAQEVTDLFAHEQAGNVDGVGDVTFYTDADED
                     GYGDPATITTDCEQPPGTVLVGGDCDDSNPDIHPGASEICDGIDNDCANGVDDGLTVT
                     TWYADADNDGFGDPNSSIQNCEEEAPAGYVANNTDLCPADINKQAPGACGCGVPETDS
                     DTDGTPDCIDGCPNDPLKTAPGQCGCGNADNDTDNDGIADCVDTCPGVTGQIGSACDD
                     GLATTGNDVLDNACECHGLLIDCLGMPGGAALPGTACTVSNLPGIWSASCVCELEGPD
                     VAVQNVSAVNDTIAPDQSITLNWNVANLGNAAATVNWTERYYMESGSGANRTLIAQAP
                     YTAGT"
BASE COUNT          407 a          805 c          830 g          542 t
ORIGIN      
        1 gtgtgcctgc agtgtagggg gcttgcgcga tcagcgtgcg gttcgcgccg ctgcccgatt
       61 ccatgtagta gcgctcggtc cagttcaccg tggcagcggc gttgcccagg ttggctacgt
      121 tccagttcaa tgtgatcgac tggtctgggg cgatggtgtc gttcaccgcg ctcacgttct
      181 gcacggccac atcggggcct tccaattcgc acacgcaact ggccgaccag atgccgggca
      241 aattgctcac ggtgcatgcc gtgccgggca gggccgcgcc tcccggcatg ccgaggcaat
      301 cgatcagcag gccgtggcac tcgcaggcat tgtcgagcac gtcgttgccc gtcgtggcaa
      361 ggccatcatc gcaggcggag ccgatctggc cggtcacacc aggacaggta tccacgcaat
      421 ctgcgatgcc atcattgtcc gtgtcgttgt ccgcattgcc gcagccgcat tggccgggtg
      481 cggtcttcag cggatcgttg gggcagccgt cgatgcaatc aggtgtgcca tcggtgtcgc
      541 tgtcggtttc gggcacgccg caaccgcagg cacccggagc ttgtttgttg atgtccgcag
      601 ggcagaggtc tgtgttattg gccacgtagc ccgcgggcgc ctcctcctcg caattctgaa
      661 tgctgctgtt cgggtcgccg aagccgtcgt tgtcagcgtc ggcgtaccag gtagtgaccg
      721 taaggccatc gtccacgcca ttcgcgcagt cgttgtcgat cccgtcgcag atctccgatg
      781 cgcccggatg gatgtccggg ttgctgtcgt cgcaatcgcc gcctacgagc acggtgccag
      841 ggggctgctc gcaatctgtg gtgatggtcg ctggatcgcc gtagccgtcc tcatcggcat
      901 cggtgtagaa ggtcacatcg cccacgccat ccacattgcc tgcttgctca tgcgcgaaca
      961 ggtcggtcac ttcctgcgcg gtgagtgccc ggttgtacag gcgcagattg tccattttgc
     1021 cgctgtagaa ctgtatggcg ctcgccgagt tccggccaat gaagagggcg atgcttgttg
     1081 tgatgttctc cacgggatgc gtggccgtcg cattcagcac gccatccaca tatatgcgca
     1141 tggtcgagcc cgtgcgggtg tgcacgatat ggtgccaggc gttgttgccc aggccgccgg
     1201 agctcgatac tgcctttgat gagttgttgc gtccatggaa tactcctgcg ctcggatggt
     1261 caacgaaaca ctgcaatccg cgatacggtg aacagcacga ctcgcctttg tcgaagatgt
     1321 tgccaaggct cccggcagcc caattcaacc acaccgaata ggtgtagtct cctgtgccca
     1381 ggttgagcga gttgctatgg ggcaccagca cgtaacggct tccaccgaac tggaaggccc
     1441 cgttggcggt gccatccatg ccagtggtga acgaagcgcc acccggtgct gtgccgttgt
     1501 tgccattggt agagtggtcg ttcaggttgc cattgaagag ccattgtgct acaagcccat
     1561 tgctgaatgt ggtgggatca tccaccgttc cgttgcaatc gttgtcgaag ccatcgcaga
     1621 tctcgatggc gcccggatgc atcgcgttgt tgttgtcgtc gcaatcaccg gccgtggcgg
     1681 agtagcccgc aggcaccgtg caggtgaaga agcccggcac cagcgcgccc gtgccgtagc
     1741 catcgccatc ggcatccacg tagtagctcg cgggcacatc ggccacgccg cagccgcatt
     1801 cgccgggcgc gatcttgttc acatcgttgg ggcagccatc gtccgcgttg caggtgccgt
     1861 cgttgtcgct gtcctggaag atgcccgcgc actggcaatc cacgccgatc acatcgttcg
     1921 tggtgcaggc gctgccgtca tcgcagggcg agccgggcgt aacgccgccg gggcaggcga
     1981 agggcagatt gctctcgcca tccgccaccg tgaactcacg gaagctgctc acgcccgcca
     2041 acgagcgggt gtacgggttg ctgccgctcg ccccgcccaa tgcgccggcc acccaatcgg
     2101 tgccgttgta ggcgtgcagc gcgcaattgc tccttccgaa gttcgctccc tcatcgaccg
     2161 tgttccactg cagttgcacc gtggcctggt tgccgccgct ggtctgctcg gtgatcgtcc
     2221 aggtgcgctc cacctgctcg ttcaccacca agtcgccggt gggcacgccg ggcgcgccgt
     2281 actcactgta cacttcgttc tgcacacgcg ccgcgaaagt ctcctgcgga ccgctcgtga
     2341 ggctgagcgt ggcgggcagg tagctggtgg ccgtgccgat ggggaacagc acattggagc
     2401 cgttgatcgc ccggcgcaat gaacccatgc cgtcggtaac gaggtagcgt gcggccgact
     2461 ggttcgtgat cgtggcggcc agggccagcg tgaggttgtt cgcgccgagc gtgagcttgc
     2521 cattggtgag cgtgaggttc gtattgatcg tctggtcgcc gcccagcgat acgccattgg
     2581 catt
//
LOCUS       DAOQOC010000349         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22920_length_2583_cov_4.367880, whole genome shotgun sequence.
ACCESSION   DAOQOC010000349 DAOQOC010000000
VERSION     DAOQOC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22920_length_2583_cov_4.367880"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1789)
                     /locus_tag="PL106_06575"
     CDS             complement(254..1789)
                     /locus_tag="PL106_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HNA32769.1"
                     /translation="MKNGSARTRTKAKKRTPNLLKGISDIRRFFHRNETPLYFISATN
                     FNLLGADEWIKGFKFITYIDCFDGMHPNLMSPRTEEPHEEFQSIEDINNYLLTHSEVR
                     DYIESRRSTTGPDRQYPNGKALFLMFDEKTEALAKKAGLEVIFPKAKLRTWLDNKVNT
                     NRVAEKAGVPCVPYVLSPVRHYEHLLAVAQKGKLGNDLVVQTPYGDSGHTTFFIKSAS
                     DYDKYAKEIEAEKECKIMKRINCRGAAMEACVTRYGTIVAPLMTELVGFKELTPYKGG
                     WCGNEIHADTFTPEIRAKARRYARKFGDQLLKEGYKGYFELDYLIDKDSGRIYLGELN
                     PRITGASSITNHAAFAMADAPLHLFHTLEWMNVPYQLSVTALNRRWSDARNIDSWGQM
                     VIKHTRDDIRRVTEAPRSGIWRMREDGSIYFWRMDTHRRYVEKDNEAFFLRITRAGDW
                     LYEGADIGILVMRGRMMTDDFQLTERSKMWLAAIRAEYKSIAPGEQAGTGHMLEIGGF
                     KMM"
     gene            2041..2448
                     /locus_tag="PL106_06580"
     CDS             2041..2448
                     /locus_tag="PL106_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32770.1"
                     /translation="MTRLAQMSLTAPGLVEQRFHAGAGLDAEGFEENRRARFELVGDT
                     PHVMLSIFPASMDFNLNVATSDHFGKWKGRDGLKALAVVVRDGATEGIVKLHFAYYPA
                     WFPVRTFNNEAEARTWLIGQLEYLEPGTEEAGL"
BASE COUNT          435 a          785 c          803 g          560 t
ORIGIN      
        1 agcacgctcc ggcaggtcac caggtcggga cgcgcgtcca gtccttcgct ctcgtaccag
       61 atcttgaaca ggggccaggc atggtcgaag aacttctgcc agcggtcgct gggccagggt
      121 tccttgacga gtttcaaatg aacgtacatc aggctcactt cgttcgttgc gtggttcgtg
      181 gcgagtgggg ggggccaacc gcggaagcgg tcccgccacc cctcacccgc cacccgtcat
      241 tccccactcg cattcacatc atcttgaatc caccgatctc cagcatgtgc ccggtgcctg
      301 cttgctcgcc gggagcaatg ctcttgtact ccgcacggat cgcggcgagc cacattttcg
      361 agcgctcggt gagctggaag tcgtcggtca tcatgcggcc gcgcatcacc aggatgccga
      421 tatcagcgcc ttcgtacagc cagtcgccag cgcgcgtgat gcgcaggaag aacgcctcat
      481 tgtccttctc aacgtaccgg cggtgcgtgt ccatgcgcca gaagtaaatg cttccatcct
      541 cgcgcatgcg ccagatcccg ctgcggggag cttcggtcac acggcggata tcatcccgcg
      601 tgtgcttgat caccatctgg ccccagctgt cgatgttgcg cgcatcggac cagcggcggt
      661 tcaatgccgt cacgctgagc tggtagggga cgttcatcca ctcaagcgtg tggaacaggt
      721 gcagtggcgc atcggccatg gcgaaagcgg cgtgattggt gatcgaactc gcaccggtaa
      781 tgcgcggatt cagttcgccc aggtagatgc ggccgctgtc cttatcgatg aggtagtcca
      841 gttcgaaata gcctttgtac ccctctttca gtagctgatc accgaatttg cgcgcgtacc
      901 ggcgggcctt ggcgcggatc tccggcgtga aggtgtccgc atggatctca ttgccgcacc
      961 agccgccctt gtacggggtg agttccttga agcccacgag ttcggtcatc agcggggcca
     1021 cgatggtgcc gtaccgggtg acacaggcct ccatggccgc gccccggcag ttgatccgct
     1081 tcatgatctt gcattccttc tccgcctcga tctccttggc gtacttgtcg tagtcggatg
     1141 cgctcttgat gaaaaaggtg gtgtggccac tgtcgccgta cggcgtttgc accacgaggt
     1201 cgttgccgag cttgcccttc tgcgcaacgg ccagcaagtg ctcgtaatgc cgtacggggc
     1261 tcagtacata gggcacgcat ggcactccgg ctttctcggc cacacggttc gtgttcacct
     1321 tgttgtcgag ccaggtgcgc agcttcgctt tcggaaagat cacctccagc ccggcctttt
     1381 tcgccagcgc ctcggtcttc tcatcgaaca tgaggaagag ggctttgcca ttggggtatt
     1441 gccggtccgg tcccgtcgtc gaccgccggc tctcgatgta gtcgcgcacc tcgctgtgcg
     1501 tgagcaggta gttgttgatg tcctcgatgc tctggaactc ctcgtgcggc tcctcggtgc
     1561 gcgggctcat caggttgggg tgcatcccat cgaagcaatc gatgtacgtg atgaacttga
     1621 accccttgat ccattcatcg gcaccgagca gattgaagtt ggtggcgctg atgaagtaca
     1681 gcggggtctc gttgcggtgg aaaaagcggc ggatgtcgct gatgcccttg agcaggttcg
     1741 gggtcctctt cttcgccttc gtacgtgttc gagccgatcc gttcttcacc gatcctgacg
     1801 atgggcccct gggcggggct gtcttgtggg cggaccgctt cgccgatgcg cggcgggtag
     1861 gtgtcttctt ctgggccatg tgatcccggt cgtctccgga tggccgaagt acggcactgt
     1921 gcgccgtagg aacaacaccc gggccggacg atacgccgtg ttccgccaag tcgtagtctt
     1981 gtgggatgct taaaaccacc ggtcatgccc cactctcccg ggatgcctcc gcgcacgatc
     2041 gtgacacggc tcgcacagat gagcctgacc gcaccgggcc tggttgaaca gcgcttccac
     2101 gcgggtgccg ggctggacgc cgagggcttc gaggagaatc ggcgcgcacg cttcgaactg
     2161 gtgggggata caccccatgt gatgctcagc atttttcctg cgagcatgga tttcaatttg
     2221 aacgtggcca cttcggacca cttcggcaaa tggaagggcc gggatgggtt gaaggcgctg
     2281 gccgtcgtgg tgcgggacgg agccacggag gggatcgtca agttgcactt cgcgtactac
     2341 ccggcatggt ttccggtgcg caccttcaac aacgaggcgg aagcgcgtac ctggttgatc
     2401 gggcagttgg agtatctgga gcccggaacg gaagaggccg gtctgtgacc ggcctctctc
     2461 atgatcctca agtaccgggt cactccacga tcaggcgccc tgtccaatgg gcggacttgc
     2521 cggtgacccg cacgaaatac atgcccggtc cgttcgcgct cagatccagc ggaacgagtg
     2581 atc
//
LOCUS       DAOQOC010000350         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_22925_length_2583_cov_3.570016, whole genome shotgun sequence.
ACCESSION   DAOQOC010000350 DAOQOC010000000
VERSION     DAOQOC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22925_length_2583_cov_3.570016"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1138
                     /locus_tag="PL106_06585"
     CDS             185..1138
                     /locus_tag="PL106_06585"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNA32771.1"
                     /translation="MNDHEAVSAHPGVQAASEVILVRPSGFGFDQATAATNGFQRFSA
                     DADIRFRAEEEFDGLISALDRCGIGITVLDPFDPKAPNAVFPNNWFSTHADGTLVLYS
                     MCAPSRRTERDPAFDLTLKREGFVVRKLMDLSPLEHGEKFLEGTGSLVLDRTRNIAYA
                     ALGPRTSERGINLWCGGMNASPVTFHATMDGTLAGAPVYHTNVMMSIAERFALVCMEA
                     LPYPVDRDDLWAELDRSEREIVPITLAQMHAFCGNILELRSNKGSFIFLSESAFHALK
                     PDQRVSLEKHAQFVPVPVPVIERVGGGSVRCMIAENFLPIG"
     gene            complement(1193..1840)
                     /locus_tag="PL106_06590"
     CDS             complement(1193..1840)
                     /locus_tag="PL106_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA32772.1"
                     /translation="MKQKIGVLGTGMVGQVLATGFLDHGHDVMIGTRTPQKLEEWLDK
                     NPTGRVGGHRETGAWADLLVLAAKGSAAETVVRLLDSKDVQGKIIIDATNPLADAPPE
                     NGVLKYFTSLENSLMEQLQAAQPGARFVKAFNSVGNAYMVDPKFEEGRPTMFICGNDA
                     EAKGTVAALLNSFGWDAEDMGGVEAARAIEPLCMLWCLPGFTRNQWSHAFKLLKR"
     gene            1925..>2583
                     /locus_tag="PL106_06595"
     CDS             1925..>2583
                     /locus_tag="PL106_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA32773.1"
                     /translation="MSTAVRIAYDDLDLPLVVVVGGGFAGLELIQQLDGKPYKVLLLD
                     KQNHHCFHPLLYQVATASLSADSIAHPFRRTVAPMRNVAFRMACATAVDPVRKVVITD
                     HGEFPYDILVLATGSRTNFFGNSAIGSGAMRLKSISQALDIRSDFLQDFEAALYAQDE
                     TDQRRCLNFTIVGAGPTGVELAGALAEIKNTVLKREYRELHSDRMRIWLVDSNDRVLA
                     H"
BASE COUNT          466 a          820 c          759 g          538 t
ORIGIN      
        1 ctatcactgc cggaagtccg caccctgctt acgggattat gtgttgacgc gaagaccgct
       61 accctggatg tggtggagat caacccggcc ttggatacca acaatgccat ggccgaggct
      121 gtgctcacgg tgcttgaacc gctgttcccc atcctgcgca accgctgatc cacgatccgg
      181 gtaaatgaac gatcacgaag ctgtatcagc ccatccgggt gtccaagccg catcggaggt
      241 catcctggtc cggccgtcgg gcttcggctt tgatcaagca acagccgcca ccaatgggtt
      301 ccagcggttc tcggccgatg cggatatccg cttccgcgcc gaagaggagt ttgatggctt
      361 gatctcggcg ctcgaccgat gtggcatcgg tatcacggtt ctcgacccgt tcgaccccaa
      421 ggcgcccaac gcggtttttc ccaacaattg gttcagcacg cacgccgacg gcacgttggt
      481 gctctactcc atgtgcgcac cgagccgacg taccgaacgg gatcccgcct ttgacctcac
      541 gctcaaacgc gaaggattcg tggtgcgcaa actgatggac ctctcgccgc tggagcacgg
      601 tgaaaaattc ctggaaggca ccggcagcct ggtccttgac cgcacgcgca acatcgctta
      661 tgccgcgctc ggcccacgca cttccgaacg cgggatcaac ctgtggtgtg gtggcatgaa
      721 cgcttcaccg gtcaccttcc acgccaccat ggatggcacc ctggccgggg cgcccgtcta
      781 ccacaccaac gtgatgatga gcatcgccga gaggttcgcc ctggtctgca tggaggcctt
      841 gccgtatccg gtggatcggg atgacctctg ggccgagttg gaccgttcgg aacgcgagat
      901 cgttccgatc accttggccc agatgcatgc cttctgcgga aatatcctgg agttgcgatc
      961 gaacaagggc tccttcattt tcctctctga atcggctttt catgccctga agcctgatca
     1021 acgggtttcc ctggagaagc atgcccagtt cgtgcctgtg ccggtcccgg tgatcgaacg
     1081 ggtgggaggg ggcagtgtgc gctgcatgat cgccgagaac ttcctgccca tcggttaacc
     1141 cccgctgtgg cacggcccca tgccgtgccc gatggagttc tgttggccgg gcctatcgct
     1201 tgagcagctt gaacgcatgg ctccactggt tccgtgtgaa gcccggcagg caccataaca
     1261 tgcagagcgg ctcgatcgca cgagcggcct ccacaccgcc catatcctcg gcatcccagc
     1321 cgaagctgtt caacagggcg gccaccgtgc ccttcgcctc ggcatcgttg ccgcaaatga
     1381 acatggttgg acggccctct tcgaatttgg gatccaccat gtacgcattg cccacgctgt
     1441 tgaaggcttt cacgaagcgt gcgccgggtt gagcggcttg caattgctcc atcaggctgt
     1501 tctccagtga ggtgaagtac ttgagcacgc cgttctccgg tggcgcatcg gccaaggggt
     1561 tcgtggcgtc gatgatgatc ttgccctgca catccttgct gtccagcaag cgcaccacgg
     1621 tttctgcggc cgaacccttg gcggccagaa ccagcaggtc ggcccaggcc ccggtctccc
     1681 ggtgcccccc gacacggccc gtcggattct tgtccaacca ctcctcgagc ttctgcgggg
     1741 tccgcgttcc gatcatcacg tcgtggccat gatcgaggaa acccgtggcg agcacctggc
     1801 ctaccatgcc agtgcccaga acgccgatct tctgcttcat ccgtgttggg acgggagttt
     1861 gctttgcccc gttccgcaaa ggaatgcacg gcgcgtgatc cgtgccgatc tttgcggtgc
     1921 caccatgtcc accgcggtta ggatagctta cgatgacctt gatctccctc tggtggtggt
     1981 ggtgggtggg ggcttcgccg ggcttgaact cattcagcaa ctggatggaa agccgtacaa
     2041 ggtgctgctg ctcgacaagc agaaccacca ctgctttcat ccgctgctgt accaggtcgc
     2101 aacggccagc ctgagcgccg acagtatcgc gcacccgttc cgccgtaccg tggcgcccat
     2161 gcgcaacgtg gcctttcgta tggcgtgtgc gaccgcagtg gatcccgttc gcaaggtggt
     2221 gatcaccgat catggggagt tcccctacga catcctggta ctggccaccg gaagtcgcac
     2281 caacttcttc ggcaactccg ccattggatc cggtgcgatg cggttgaaga gtatcagtca
     2341 ggcgctcgac atccgcagcg acttcctcca ggatttcgaa gcggcgctgt acgcgcagga
     2401 cgaaacggat caacgacgct gcctcaattt caccatcgtg ggtgcgggtc ccaccggtgt
     2461 tgagctggcc ggtgcgctgg ccgagatcaa gaacacggtg ctcaagcgtg aataccgcga
     2521 actgcatagt gatcggatgc gcatctggct ggtggacagc aacgatcgtg tactggccca
     2581 ttt
//
LOCUS       DAOQOC010000351         2577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23044_length_2577_cov_3.019033, whole genome shotgun sequence.
ACCESSION   DAOQOC010000351 DAOQOC010000000
VERSION     DAOQOC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23044_length_2577_cov_3.019033"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..802
                     /locus_tag="PL106_06600"
     CDS             230..802
                     /locus_tag="PL106_06600"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNA32774.1"
                     /translation="MNKLNIVLFGPPGAGKGTQSEFLIKKFGLVHLSTGDLLRAEIKD
                     ETPLGLQAQEIMSRGELVSDHIVIGMIRNRIDANNTAPGFIFDGFPRTKAQAEALDEM
                     LNAKSQPITAMLALEVPGPELVRRLLKRGETSGRSDDRDEQVIANRIAEYEQKTSPLK
                     DYYSAQGKYKGINGVGSVADITERLVEVIG"
     gene            822..1829
                     /gene="obgE"
                     /locus_tag="PL106_06605"
     CDS             822..1829
                     /gene="obgE"
                     /locus_tag="PL106_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNA32775.1"
                     /translation="MSGTPNFIDHIRIEARSGKGGAGSKHLRREKYMPKGGPDGGDGG
                     RGGNVILRGNSQLWTLLHLRYTKHVIAGDGEVGSGNQCSGRSGKDVVVEVPLGTVAKD
                     DETGEVLFDITENGQETILLEGGRGGLGNQHFHSSTFQTPRFAQPGEPGQQKWFKLEL
                     KVLADVGLVGFPNAGKSTLLASISAAKPKIADYPFTTLVPNIGIVPYHDNRSFVMADI
                     PGIIEGASEGKGLGLRFLRHIERNSVLLFMIPCDSTDIKKDFRILKNELEQFSPELLH
                     KDIVLAITKCDMLDAELTKAIRKELPRKVDCVLISSVANQGLGELKEVLWRKLHPLSD
                     E"
     gene            1909..2490
                     /locus_tag="PL106_06610"
     CDS             1909..2490
                     /locus_tag="PL106_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNA32776.1"
                     /translation="MSLGQAILQADRSAFLVINGAHSPGADTFFWYVSDPRIWFPVYV
                     LFLILIKLRWGWRGLWWSLPVAALMILCTDTGSVVLFKNTVQRLRPTHEPALTGLVHT
                     VRGHLGGDFGFVSTHAANHFGLAAFMTGILLRKPWWTLWLLLTWAALIGYSRVYLGVH
                     YPGDVIVGGLYGAIIGLLAFRLFLWLHERRANA"
BASE COUNT          495 a          816 c          814 g          452 t
ORIGIN      
        1 ggcccgggtt cacgctcctc gtcggaggca aggcgaagga cctgcccatc gagcacgtgg
       61 ccatgcgcat ccccaacgat ttcgtggtcg gcagcgggct cgatcacgat ggcctcggcc
      121 gtaacctgcc cggcatcctg cgcctcatca aggactgaac accggccgcc tgaacacatt
      181 gaccagggat gttcccgttg atcgaccggc ggccacggca agacgaacca tgaacaaact
      241 gaacatcgtg ctcttcggcc cccccggtgc gggcaagggc acccagagcg aattcctcat
      301 caagaagttc ggcctggtac acctgagcac cggcgatctg ctgcgtgccg agatcaagga
      361 tgaaacgccc ctcggcctcc aggcccagga gatcatgtcg cgtggtgagc tggtgagcga
      421 ccacatcgtc atcggcatga tccgcaaccg gatcgatgcg aacaataccg cgccaggttt
      481 cattttcgac ggcttccccc gcaccaaggc ccaggccgag gcgctggacg agatgctcaa
      541 tgccaagagc cagcccatca ccgccatgct cgccttggaa gtgcccgggc ccgagttggt
      601 gcggcgactg ctcaagcgtg gtgaaacgag cggccgttcc gatgaccgtg acgaacaggt
      661 gatcgccaac cgcatcgccg agtacgagca gaagacttca ccgctgaagg actactacag
      721 cgcccaaggc aagtacaagg gcatcaacgg cgtgggctct gtcgcggaca ttaccgaacg
      781 gctggtagag gtgatcggct gaaaggcaag ttcccgatcc catgtccggc acccccaact
      841 tcatcgacca catccgcatc gaggcccgca gcggcaaggg cggggcgggc agcaagcact
      901 tgcgccgcga gaagtacatg cccaagggcg gccccgatgg cggcgacggc gggcgcggcg
      961 gcaacgtgat cctgcgcggc aacagccagc tgtggacgct gctgcacctg cgctacacca
     1021 agcacgtgat cgcgggtgac ggtgaggtgg gcagtggcaa ccagtgcagt ggtcgaagtg
     1081 gcaaggacgt ggtggtcgaa gtgccgctgg gcaccgtggc taaggacgac gagaccgggg
     1141 aggtgctgtt cgacatcacc gagaacggcc aggaaacgat cctgctggag ggtggtcgag
     1201 ggggtctcgg aaaccagcac ttccatagct ccacgttcca gacgccccgc ttcgcgcaac
     1261 ccggcgagcc gggccaacag aagtggttca aactggagct gaaggtgctc gcggatgtgg
     1321 gcctggtggg cttccccaac gctggcaaga gcacgctgct ggccagcatc agcgcggcca
     1381 aaccgaagat cgccgactat cccttcacca cgctggtgcc caacatcggc atcgttccat
     1441 accacgataa caggagcttt gttatggccg atatccccgg tatcatcgaa ggggccagcg
     1501 aaggcaaggg gctcggcctg cgtttcctgc gccacatcga gcgcaacagc gtcctgctct
     1561 tcatgatccc ctgcgacagc accgacatca agaaggactt caggatcctg aagaacgaat
     1621 tggaacagtt cagccccgaa ctgctgcaca aggacatcgt gctggccatc accaagtgcg
     1681 acatgctcga tgccgaactc accaaggcca tccggaagga actgcccagg aaggtggatt
     1741 gcgtgctcat cagctccgtg gccaaccagg gcctgggcga gttgaaggag gtgctgtgga
     1801 ggaagttgca tccgttgagt gacgagtgac gagtggcgag tggcgaacct gccgtggcaa
     1861 ctgccactcg ccactcgtct ccccgcactc gcgatattcg ccctcgccat gtccctgggc
     1921 caagccatcc tgcaagccga ccgttcggcg ttcctggtga tcaatggggc gcactcaccg
     1981 ggagcggaca cctttttctg gtatgtgagc gatccgcgga tctggttccc cgtgtacgtg
     2041 ctgttcctca tcctgatcaa actgcgctgg ggttggcgcg ggctctggtg gtcactgccg
     2101 gtggccgctc tgatgatcct gtgtaccgac accggttccg tggtgctctt caagaacacc
     2161 gtgcagcggc tgcgacctac gcacgaaccg gcgctcaccg gcctggtaca cactgttcgg
     2221 ggccatttgg gcggcgattt cggcttcgtc tccacgcatg cggccaacca cttcggcctg
     2281 gccgcgttca tgacgggtat cctcctgcgc aagccgtggt ggacgttgtg gctcctgctc
     2341 acctgggccg ccctgatcgg atacagtcgc gtctatctcg gtgtacacta cccgggtgat
     2401 gtgatcgttg gcggtctgta cggcgcgatc atcggcctgc tggccttccg cctcttcctc
     2461 tggttgcacg aacgacgcgc gaacgcatga acctgtggct ggcggtcttc ctgggcggcg
     2521 gtgcgggcag cgtcgcgcgc ttcgcgctca gccggatcct gatgccggtg gaggggg
//
LOCUS       DAOQOC010000352         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23065_length_2576_cov_3.095597, whole genome shotgun sequence.
ACCESSION   DAOQOC010000352 DAOQOC010000000
VERSION     DAOQOC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23065_length_2576_cov_3.095597"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PL106_06615"
     CDS             <1..237
                     /locus_tag="PL106_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HNA32777.1"
                     /translation="ATALLAWLIQRFEVHLPQKEQWRALLAVGFCGGFSTLSTFSYEN
                     FRLLRDGLYGFATLNILVSVVAGILLFHLFARSA"
     gene            234..1988
                     /locus_tag="PL106_06620"
     CDS             234..1988
                     /locus_tag="PL106_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNA32778.1"
                     /translation="MNRILRITGSMFMPRFIGVARAWPLVFFFLATIRTEAQSGKLPV
                     AVPRAEMMTTPAWTTPPKLVVGIVVDQMRVDYIYRYWDNFGSGGFKRLIGGGAFLRDM
                     HYNYAPTHTGPGHASIYTGTTPHDHGIVANDMFIRSTGKGLNCVQDDATSGVGAEGPR
                     AQRSPVNLRSSTLADEMERRYEGRSKTIGVAWKDRAAILPIGRTGDAAYWNFEDAEGR
                     FATSRWYMESLPDWLVRFNARRLPAEYLKGTWDLLLPRERYHQSMPDDNPYEELLPGV
                     EHPVLPLDLARAFAASGNNMGLLKTVPAGLTLVTELAMAALEGEGMGRDEVPDLLSVS
                     YGGTDEIGHATGPLSLEVEDMYLRLDRELERLLTGLDERIGQGGYTVFLTADHAGGDV
                     PEYLKSRKASAGYVEMAELTAAVDAALMQRFGTGKWVRKRVKEQFFLNDSLILANKLD
                     RALVQDVAAAAVRAFPGVADAYTAENLVRGTRLAGRAALVQRGFFFERSGDVAFILRP
                     GYLSAWPGMARQGADHGSVWNYDTHVPTIFFGHGVVKGEVVRRTSITDIVPTICMILG
                     CSLPDAAIGEPVREVLQR"
     gene            2107..2511
                     /locus_tag="PL106_06625"
     CDS             2107..2511
                     /locus_tag="PL106_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32779.1"
                     /translation="MVDQDPGFFNPHFHIEESEDVLVLLLGNSAPIDESIMKDILRAV
                     HRRDPIGGMPILVQQDELVRMTPEAKAFLARTCDNRSRPVAFMAYDLPDRIQGDFFLR
                     FHKPRFPFRVFSVVDDAQQWFAGFTASKYAVR"
BASE COUNT          460 a          806 c          811 g          499 t
ORIGIN      
        1 gccaccgcgc tgctcgcctg gctgatccag cggttcgaag tgcatcttcc gcaaaaggag
       61 cagtggcgtg ccttgttggc ggtaggtttc tgcggaggct tcagcacgct cagcaccttc
      121 agctacgaga acttccgcct gctccgcgat ggactttacg gcttcgccac gttgaacatc
      181 ctcgtcagcg tggtcgccgg tatcctgttg ttccacctct tcgcccgttc cgcatgaatc
      241 gcatcctccg gatcaccggc tccatgttca tgccccgctt catcggggtc gcccgggcct
      301 ggccgttggt cttttttttc ctggccacga tccgcacaga agcccagagc gggaaactgc
      361 ccgtggccgt gccgcgtgcg gaaatgatga caacgccggc atggaccacc ccgcccaaac
      421 tggtcgtggg tatcgttgtg gaccagatgc gcgttgatta catctatcgg tactgggata
      481 atttcggttc cggcggattc aagcggctca tcggaggagg ggcattcctg cgcgatatgc
      541 actacaacta tgcacccacc cacaccgggc cgggccatgc gagcatctac accggcacca
      601 ccccgcacga tcatggcatc gtggccaacg acatgttcat ccgcagcacc ggcaaagggc
      661 tgaactgcgt gcaggatgac gcgaccagcg gtgtgggcgc cgagggccca cgggcgcaac
      721 gatcgccggt gaacctgcgc agcagcaccc tggccgatga gatggagcgg cgttacgagg
      781 gccgctcgaa gaccatcggc gtggcctgga aggaccgggc cgccatcctg cccatcggaa
      841 ggacgggcga tgccgcatac tggaacttcg aggatgccga aggacgcttc gccaccagcc
      901 gatggtacat ggaaagcctg cccgactggc ttgtccgatt caacgcacgc cggttgcccg
      961 cggaatatct caaaggcacc tgggacctgt tgctcccgcg cgaacggtat catcaatcca
     1021 tgcccgacga taatccgtac gaggaactcc tgccgggcgt ggagcatcct gtgttgcccc
     1081 tcgaccttgc ccgcgccttc gccgcgagcg ggaacaacat ggggctgctg aaaacggtcc
     1141 cggccggtct caccctcgtt accgaactcg cgatggcggc cctggaaggg gagggcatgg
     1201 ggcgtgatga ggtgcccgac ctgctctcgg tgagctacgg tggtaccgat gagatcggac
     1261 atgccacggg gccgctctcc ttggaagtgg aggacatgta tctccggctc gatcgcgaac
     1321 tggagcgcct gctgaccgga ctggacgaac ggatcggcca ggggggatac accgtctttc
     1381 tcaccgcgga ccatgccggg ggtgatgtac cggaatacct gaaaagcagg aaggccagtg
     1441 cgggctatgt ggagatggcg gaactgacgg cagcggtgga tgccgcattg atgcagcgat
     1501 tcggtaccgg aaaatgggtg cgcaagcggg tgaaggagca gttcttcctg aatgactcgc
     1561 tcatcctggc gaacaagctc gaccgggccc tggtgcagga tgtggccgcc gctgccgtgc
     1621 gtgcgttccc gggtgtggcc gatgcctaca cagcggagaa tctcgtacgc ggaacgcgac
     1681 tcgccggacg tgccgccctc gtgcaacgcg gtttcttctt cgagcgcagc ggcgatgtcg
     1741 ccttcatcct acgtcccgga tacctctcgg catggccagg catggccagg cagggggccg
     1801 atcacggttc ggtgtggaac tacgacaccc atgtgcccac catcttcttc ggccacggcg
     1861 tggtgaaagg ggaggtggta cgtcgaacat cgatcacgga catcgtcccc acgatctgca
     1921 tgatccttgg ctgctcgttg cccgatgcgg ccataggtga gcccgtacgc gaggtgttgc
     1981 agcgttgatc ctcctgaccg gcacacggat ctccctgtgc tggcgacccg ggatcttcac
     2041 gttgccctgt tcacacttag cttccacgga cgttggccgg tatgatggac cgggtagctt
     2101 tcgctcatgg ttgatcagga tccggggttc ttcaacccgc acttccacat cgaggagtcc
     2161 gaagacgtgc ttgtgcttct gctgggcaat tcggctccca tcgacgagag catcatgaag
     2221 gacatcctcc gtgccgtgca tcgcagggac cccatcggcg gcatgcccat cctggtgcag
     2281 caggatgagc tggtgcgcat gacgcccgag gccaaggcat tcctggcgcg tacctgcgac
     2341 aatcgttcgc gcccggtggc cttcatggcc tacgacctcc cggaccggat ccagggtgat
     2401 ttcttcctgc ggttccacaa accccggttc ccgttccgtg tcttctccgt ggtggatgat
     2461 gcgcaacagt ggttcgccgg attcacggcc tccaagtatg ctgtgcggta gcccgcaagg
     2521 gtggcgatca acccgatccc ccggccggtc gaacacgggc tgttgataag agtggg
//
LOCUS       DAOQOC010000353         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23104_length_2573_cov_3.468229, whole genome shotgun sequence.
ACCESSION   DAOQOC010000353 DAOQOC010000000
VERSION     DAOQOC010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23104_length_2573_cov_3.468229"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PL106_06630"
     CDS             <1..219
                     /locus_tag="PL106_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32780.1"
                     /translation="DINTTNCISKFSKDDSRKVRFDVELLPQNEDKNLAFVAPVGHVT
                     FAKGGLLKVSYDHNLVTLPVQENEVASK"
     gene            381..1466
                     /locus_tag="PL106_06635"
     CDS             381..1466
                     /locus_tag="PL106_06635"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32781.1"
                     /translation="MKQTLPLLVALTLASQAVAQCSVDSTFAQSGPGLYPQGPMSPDC
                     DLLASKTVVGITDTTIANPLGGGDEVTVYLTGIRINAILGLPAGLTLETDVMDSADEE
                     GQWGYWYNSGSVPEQNSALGCGHVVGSTADWEAAAGGGPNNDGVYPLEFTIDAYVEST
                     DNALLNQFLQPQWLSALGDLGPGAFVVFDTLVILPGFNTIAASIIGADNADAGSSYIY
                     STDAIGDTYDWTVTNGTIVSGQGTDSIEVVWDVTGQVAVNAINNACSGADTLDVTVIN
                     TAMAEASRTRTVVFPNPSEGLFHVLLPDDGSVDVRILDMNGSVVRTERSSGRRSLQVD
                     LRNTPTGMYILELRSATGVARECLVRN"
     gene            1564..2346
                     /locus_tag="PL106_06640"
     CDS             1564..2346
                     /locus_tag="PL106_06640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA32782.1"
                     /translation="MFPYHDENTTQRTPVITIAIIVANALMWLLVQGAGSAYALAASV
                     CDLGLIPGEVTGLAEPGTGFEMGEGLACLVDAGPQWHTILSSMFLHGSWMHLIGNMWF
                     LWLFGNNIEDSMTRGRFLVFYLLCGLGAAAAQVLANPASIVPMVGASGAISGVMGAYL
                     LIFPKVKVWCIVPLGIIPIRFTLPAWTMLLYWLGMQVLGGSIDLAKEGGGGGVAFWAH
                     VGGFAAGLILIKLFTKELAYEAHKYSYWEPKDRTRFGQGGRW"
     gene            complement(2364..>2573)
                     /locus_tag="PL106_06645"
     CDS             complement(2364..>2573)
                     /locus_tag="PL106_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32783.1"
                     /translation="HGFGRSSTGGTNWYISTLSGPVNNHVVAGNRIVLTTGTSAYAFG
                     LSSVGGSQWFVSALPSAPLGGIGTR"
BASE COUNT          461 a          780 c          803 g          529 t
ORIGIN      
        1 gatatcaaca ccaccaactg catcagcaag ttcagcaagg acgacagccg caaggtgcgc
       61 ttcgatgtgg agcttctccc ccagaatgag gacaagaacc tggcttttgt tgccccggtg
      121 ggccacgtga ccttcgccaa gggcggcctg ctgaaggtga gctacgacca caacctggtg
      181 accctgcccg tgcaggagaa cgaagtggcc tcgaaataag gttttggtgc cccggtcctg
      241 gccttgagca agtcaggaac aatgaccggg gctggtttgg cgaagagggc ggtccaacca
      301 gggccgcccc tcttcgttcg caccatggcg tcgttgggtc agggccctac tttcggcacc
      361 cgcaaccaac cacaacgttc atgaagcaaa ccttacccct tctcgtcgcg ctgacattgg
      421 cctcccaagc cgtggcccag tgttctgttg actcgacctt cgcccagtcc ggcccggggc
      481 tctatccaca gggtcccatg tccccggatt gcgacctgtt ggcttcgaag accgtggtgg
      541 gcatcaccga taccacgatc gccaacccgc ttggtggcgg ggatgaggtc accgtctatc
      601 tcaccggcat ccggatcaat gcgatcctgg gcctgcctgc cgggctcacc ctggaaaccg
      661 atgtgatgga ttctgccgac gaggaaggac aatggggcta ctggtataat agcggcagcg
      721 tgcccgaaca gaacagtgcc ctgggttgtg gtcacgttgt tggcagcacg gccgattggg
      781 aggctgcggc tggcggtggc cccaacaacg atggcgtgta cccgctggag ttcaccattg
      841 acgcctacgt ggagagcacc gataacgcac tgctgaacca gttccttcaa ccgcagtggc
      901 tcagtgcgct gggcgatctg ggccccggtg ccttcgtggt gttcgacacc ctcgtgatcc
      961 tgcccggctt caacaccatc gccgcctcca tcatcggtgc tgacaacgcg gatgcgggta
     1021 gttcctacat ctattccacc gatgcgatcg gcgacaccta cgattggacg gttaccaacg
     1081 gcaccattgt ttccggccag ggtacggatt ccatcgaggt ggtttgggac gttacgggcc
     1141 aggtggcggt aaacgccatc aacaatgcat gttccggtgc cgacacgctc gacgtcaccg
     1201 tgatcaatac cgcgatggcg gaagcgagcc gcacacgcac cgtcgtgttc cccaatccga
     1261 gcgaaggtct tttccatgtg ctcctgcccg acgacgggtc cgttgatgtg cgcatcctgg
     1321 atatgaacgg ttccgtggtc cgcacggagc ggtcttccgg gcgcaggtcg ctgcaggtgg
     1381 acctgcgcaa cacgccgacg ggtatgtaca tcttggaatt gcggtcggcc actggcgtag
     1441 cgcgcgagtg cctggtgagg aactgaccgt gtaccggtcc aactgaacgg ccccggccgt
     1501 cgcctcttac ggatgcgatg gccggggtcg gacttttgtg tacaacgcta ccttgtcccg
     1561 cacatgttcc cttaccacga cgagaatacg acccagcgta cacccgtgat caccatcgcc
     1621 atcatcgtag ccaatgcgct gatgtggctg ctggtgcagg gtgccggctc ggcctatgcg
     1681 ttggccgcca gcgtgtgtga tctggggctc attcccggtg aggtgaccgg cctggccgag
     1741 ccgggtaccg gttttgaaat gggggagggc ctggcctgtc tggtggatgc cggaccccag
     1801 tggcatacca tcctcagcag catgttcctg cacggttcgt ggatgcatct catcgggaac
     1861 atgtggttcc tctggctttt cgggaacaac atcgaggaca gcatgacgcg cgggcggttc
     1921 ctcgttttct acctgctttg cggtctgggt gccgctgcgg cccaggtgct ggccaacccc
     1981 gcgagcatcg ttcccatggt cggtgcaagc ggcgccatca gcggcgtgat gggtgcctat
     2041 ttgctgatct ttcccaaggt gaaggtgtgg tgcatcgttc cactcggcat catcccgatc
     2101 cgcttcacct tgcccgcttg gaccatgctg ctctactggt tgggcatgca ggtcctcgga
     2161 ggctcgatcg atctggccaa ggaaggtggc ggtggtggcg ttgctttctg ggcgcacgtg
     2221 gggggcttcg cagcggggct gatcctgatc aagctcttca ccaaagagtt ggcctacgag
     2281 gcgcacaagt attcgtattg ggagccaaag gacaggaccc ggttcggcca gggcggtcga
     2341 tggtgaagcg gtaattctac ctgtcaacgg gtgcctatgc cgcccagcgg agcggaagga
     2401 agcgccgaaa cgaaccattg gctgcccccg acacttgaca ggccgaacgc gtatgcgctc
     2461 gttcccgtag tgagcacgat ccggttgccc gccaccacgt ggttgttcac cggtccgctc
     2521 aaagtggaga tgtaccagtt ggtgcccccg gtggatgagc ggccgaagcc atg
//
LOCUS       DAOQOC010000354         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23201_length_2567_cov_4.233280, whole genome shotgun sequence.
ACCESSION   DAOQOC010000354 DAOQOC010000000
VERSION     DAOQOC010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23201_length_2567_cov_4.233280"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1267
                     /locus_tag="PL106_06650"
     CDS             158..1267
                     /locus_tag="PL106_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32784.1"
                     /translation="MRPSHILAALSAVVTLTIHTGCSSELDTNAPYKRITVVYSLLNK
                     NDNIHWVKINKAFLGEGDAFVYAQIPDSTEYTEEQLPESARRIEKIDANGNVIPVHPD
                     SLRLRDTIVDRPDGIFHGPAHKMYYFYGDMPGGRLDKDARYRLVLEPQGSEVITAETP
                     LVDSLAVRQTLSDPDVALSFYLGNGYQPYAVRWYTSHNARRYQAYYRFHYDEVRNGQV
                     ETKYFDNQIGTLVSDNQTGGEVSLNLNGETFYQAVANNVRYTDQSVEDQVESRIFRGV
                     EFYFWLAAPQLHTYLQLASPISGIVEERPDYSNINGGFGLMSSRMYRIVPDEAQGGNV
                     AQRKRLTSASSEQLMNGQYTGGLKFCVPTPLDGGC"
     gene            1427..>2567
                     /gene="dnaK"
                     /locus_tag="PL106_06655"
     CDS             1427..>2567
                     /gene="dnaK"
                     /locus_tag="PL106_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNA32785.1"
                     /translation="MSKIIGIDLGTTNSCVSVMEGSEPVVITNSEGKRTTPSVVAFVE
                     GGERKVGDPAKRQAITNPKNTIFSIKRLMGNSFDECAKDVARVPYEVARGENNTPRVK
                     IGDRMYTPQEISAMILQKMKKTAEDHLGTTVSEAVITVPAYFNDAQRQATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKKHKDQKIVVFDCGGGTHDVSVLELGDGVFEVKATEGD
                     THLGGDDFDQCVIDWLADEFKKDEGIDLRQDPMALQRLKDAAEKAKIELSSTTSSEIN
                     LPYIMPVNGIPKHLVRTLTRAKFEQLVDSLIKRTIAPCESALKNAGLSPSQIDEVILV
                     GGSTRIPAIQEAVKKFFGGKEPSKGVNPDEVVAIGAAIQGGVLTGD"
BASE COUNT          557 a          826 c          767 g          417 t
ORIGIN      
        1 gatccgtgcg gtcatcgtct ttggcgggtg gtgttcaggg tcaaagctac ttcctcatgc
       61 cttcctgcgg ccataccgac ctttcctatt ggtgcatccg ccattcgtgg gcttgcgcac
      121 gtcaccccgt gccggtcgat atctttggcc gcttcacatg agacccagcc acatcctcgc
      181 cgccctctcc gcggtcgtca ccctcacgat ccacaccggc tgcagcagcg agctggacac
      241 caacgccccg tacaagcgga tcaccgtggt gtacagcctg ctcaacaaga acgacaacat
      301 ccactgggtg aagatcaaca aggccttttt gggcgagggc gacgccttcg tgtacgcgca
      361 gatccccgat tccacggaat acaccgagga gcagcttccc gagagcgcac gccggatcga
      421 gaagatcgac gcgaacggca acgtgatccc ggtccatccc gattcgttgc ggttgcgcga
      481 caccatcgtg gaccggccgg acggcatctt ccacggcccg gcccataaaa tgtactactt
      541 ctatggcgac atgcccggtg gccggctcga taaggacgcc cggtatcgcc tggtactcga
      601 accccagggc agcgaagtga tcacggccga aacacccctg gtggacagcc tggccgtgcg
      661 ccagaccctc agcgacccgg acgtggcact gagtttctac ctgggcaatg gctaccaacc
      721 ttacgccgtc cgctggtaca cctcgcacaa tgcgcgccgt taccaggcgt attaccgctt
      781 ccattacgac gaggtgcgca atggacaggt ggagaccaag tatttcgaca accagatcgg
      841 caccttggta tccgacaacc agaccggtgg ggaggtgagc ctcaacctca acggcgagac
      901 cttctaccag gcggtagcca acaacgttcg gtacaccgac caatccgtgg aggaccaggt
      961 ggagagccgg atcttccggg gggtcgaatt ctacttctgg ctggccgccc cccaactgca
     1021 cacctatctt cagttggcca gccccatcag cgggatcgtg gaggaacggc cggattattc
     1081 caacatcaat ggtggcttcg gcctgatgag cagccggatg taccgcatcg tgcccgatga
     1141 ggcccagggc ggcaacgtgg cccagcgcaa acgactcacg tccgcatcga gcgagcagct
     1201 catgaacggc cagtacaccg gtggcctgaa gttctgcgtg cccacccccc tcgacggcgg
     1261 ctgctgacat cgctgacctt ttgacagcct cctaccgccc ggaatgggcg gggatcgctg
     1321 ctatttcctg ccagcatgcc gcacctgcgc gcacaggcac gatcgttgac aggccgggct
     1381 cacctatctg aaaaacgcct gcgccgggct tgcggcgttc acacacatga gcaagatcat
     1441 cggaatcgac ctgggaacca ccaacagttg cgtatccgtt atggagggca gcgagccggt
     1501 ggtgatcacc aacagcgaag gcaagcggac gacccccagc gtggtggcct tcgtggaggg
     1561 tggcgaacgc aaagtgggcg accccgccaa gcgtcaggcc atcaccaacc ccaagaacac
     1621 catcttctcc atcaagcggt tgatgggcaa ctccttcgac gagtgcgcca aggacgtggc
     1681 acgcgtgccc tacgaggtgg cacgcggcga gaacaacacg ccccgcgtga agatcgggga
     1741 tcggatgtac acaccgcagg agatcagcgc catgatcctg caaaagatga agaaaacggc
     1801 cgaggaccat ctcggcacca cggtgagcga ggcggtgatc accgtgccgg cctacttcaa
     1861 cgacgcgcag cgccaggcca ccaaggaggc gggcgagatc gccggcctga aggtgcgccg
     1921 gatcatcaac gagcccacgg cggcggcctt ggcctacggc ctcgacaaga agcacaagga
     1981 ccagaagatc gtggtcttcg attgcggtgg tggcacccac gacgtgagcg tgctcgaact
     2041 gggcgacggc gtgttcgaag tgaaagccac cgagggggac acccacctcg gcggcgatga
     2101 tttcgaccag tgcgtcatcg actggctggc cgatgaattc aaaaaggacg agggcatcga
     2161 cctgcgtcag gaccccatgg cgttgcaacg cctgaaggac gcggctgaaa aggccaagat
     2221 tgaactcagt tccaccacca gcagcgagat caacctgccg tacatcatgc cggtgaacgg
     2281 catccccaag cacttggtgc gcaccctcac gcgtgcgaaa ttcgaacagc tggtcgacag
     2341 cctgatcaag cgcaccatcg ccccgtgcga gagcgccttg aagaacgccg gcctgagccc
     2401 gtcgcagatc gatgaagtga tcctggtggg cggaagcacc cgcatccccg ctattcagga
     2461 agcggtgaag aaattcttcg gcggcaaaga gcccagcaag ggcgtgaacc cggacgaagt
     2521 ggtggccatc ggtgccgcca tccagggagg tgtgctcacc ggcgatg
//
LOCUS       DAOQOC010000355         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23243_length_2565_cov_4.131474, whole genome shotgun sequence.
ACCESSION   DAOQOC010000355 DAOQOC010000000
VERSION     DAOQOC010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23243_length_2565_cov_4.131474"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PL106_06660"
     CDS             complement(<1..779)
                     /locus_tag="PL106_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNA32786.1"
                     /translation="MSLTAAPSTSATDAFIEANKDRFLNELLDLLRIPSVSADPKYKS
                     DVARCAEAVAARLREAGAEKVEVCPTKGHPIVYGEKVVDPKKPTVLVYGHYDVQPPDP
                     LDLWHSPPFEPVIKDGLIYARGSADDKGQFYMHVKAFEAMMRSNALPCNVKFMIEGEE
                     EVGSDNLGLFVKANKERLKADVVLISDTAMLANDVPSINTGLRGLSYLEVEVTGPNRD
                     LHSGVYGGAVANPINTLCAMIASLHDADGRVNIPGFYNAVV"
     gene            1076..>2565
                     /locus_tag="PL106_06665"
     CDS             1076..>2565
                     /locus_tag="PL106_06665"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNA32787.1"
                     /translation="MKRSYASALIPLVMLAGSATAQLTVNTSQSPADLVQNVLLGGGV
                     SVSNITFNGMPANTPNFQAASFNGAGSNVGLSAGVLLATGDCGVAIGPNNNSGATAPD
                     GGVGGGGDNDLAALAGGTVFDAAVLEFDFVPSGGQIAFDFVFASEEYLEYVGAGFNDR
                     FGFFLSGPGINGPYSNNSVNIALVPGTSTPVAIDNVNNLSYPAYYVDNGDGWTEPYYS
                     GAQYIQFDGFTTPITASHEVQCGQTYHIKLAVADAGDPVLDSGVFLEAGAFASPVPVL
                     TLNDVSVPCMTEATVNLGVTGGTEPYDVTWTQNGSTVGTGMSLTVTADGTTTYQVSVT
                     DGCGGAVTGDVQVTTIAPQVTVPATLDIACGDEGALQMNLGPNGSAGLTLTWSANGSV
                     VGNAENINVPPPAQPTWYVASVSDACGGSASDSTLVSSTVPPISITVSPDVIMPCDGT
                     GATISVTGVSGGTGAIGYVWTSNGTQVGNAQDLAVGPEQAQYTVTVT"
BASE COUNT          482 a          810 c          786 g          487 t
ORIGIN      
        1 tccaccacgg cattgtaaaa acccgggatg ttgacgcgcc catccgcatc gtgcaggctg
       61 gcgatcatgg cgcagagcgt gttgatcgga ttggccaccg cgccgccgta cactccgctg
      121 tgcaggtcgc ggttggggcc ggtgacctcc acctccaggt agctgagccc gcgcaggccg
      181 gtgttgatgc tgggcacatc attggccagc atcgcggtat cgctgatgag caccacatcg
      241 gctttcaacc gttccttgtt cgccttgacg aagaggccga ggttgtcgct gcccacctct
      301 tcctcgccct cgatcatgaa cttcacgttg cagggcagcg cgttggaccg catcatggcc
      361 tcgaaagcct tcacgtgcat gtagaactgc cctttgtcat cggcgctgcc gcgggcgtag
      421 atgaggccgt ccttgatcac cggctcgaag gggggcgaat gccagaggtc caacgggtcg
      481 ggaggctgca catcatagtg accatacacc agcacggtgg gcttcttcgg gtcgacgacc
      541 ttctccccat acacgatcgg gtggcccttg gtcgggcaga cctccacctt ctccgcgccg
      601 gcttcgcgca agcgcgcggc cacggcttcg gcacaacggg ccacatcact cttgtacttg
      661 ggatccgcac tcacgctggg aatgcgcaac aagtcgagca gctcattcag gaatcgatcc
      721 ttgttggcct cgatgaaggc atcggtggca ctggtgctgg gggctgcggt aaggctcacg
      781 ggcggaaggg gttgggcgcc gaagataccc cggaagggca catgcaactt ggtggtgggc
      841 gatctcgtcc tcctgtcgag ggcacttgtc cacgaaacga cggatccccc ccatgaatgc
      901 gtggtttcct cccatgaaca ggggagggag gttgccatcg agcagataag ttcggaaatt
      961 cgtaggccct atggacagga gccagttgca caagatccag gaccacttgc tcattagcgg
     1021 aactcgggtt cgccctgatc ccgtcgtttt gccccatcga aacgcgcact ccacgatgaa
     1081 acgctcctac gcctccgccc tgatccccct ggtaatgctt gctggatcgg ccacggccca
     1141 actcacggtg aacacctccc aatcacccgc cgacctggtg cagaacgtgt tgctgggcgg
     1201 cggtgtctcg gtgagcaaca tcactttcaa cggcatgccc gcgaatacac ccaatttcca
     1261 agcagccagc ttcaacggcg cgggctccaa cgtgggcctg agcgcaggag tgctgctggc
     1321 caccggtgat tgtggcgtgg ccattggacc gaacaacaat tccggagcaa ccgctccgga
     1381 tggtggtgtc ggggggggcg gagacaacga cctggccgca ctggcgggag gtacggtgtt
     1441 cgatgcggcc gtcctcgaat tcgacttcgt gcccagcggt ggccagatcg ccttcgactt
     1501 cgtgttcgcc agcgaggaat acctggaata cgtgggtgcg ggcttcaatg accggttcgg
     1561 cttcttcctc agtggtccag ggatcaatgg cccgtactcg aacaactcgg tgaacatcgc
     1621 cctggtcccc ggaacgtcca ccccggtggc catcgataac gtgaacaacc tgagctaccc
     1681 cgcctactac gtggacaacg gcgatggttg gaccgaaccg tactactcgg gtgcgcagta
     1741 catccagttc gatggcttca ccacgcccat caccgccagc cacgaggtgc agtgcggtca
     1801 gacctaccac atcaaactgg ccgtggccga tgctggcgat cccgtcctgg attcaggtgt
     1861 tttcctggaa gctggcgctt tcgcgagccc ggtgcccgta ctgaccctga acgatgtgag
     1921 cgtgccttgt atgacggagg ctaccgtgaa cctcggcgta acaggcggta cggagccgta
     1981 tgatgtgacc tggacgcaga atggttccac cgttggcacc ggcatgagcc taaccgtgac
     2041 ggccgatggt actaccacct atcaggtatc ggtgaccgac ggatgcggcg gtgccgtgac
     2101 aggtgatgtg caggtgacca ccatagcgcc ccaagtgacc gtgcccgcca cgctcgacat
     2161 cgcctgtggc gacgaaggag ccttacagat gaacctgggg cccaacggga gtgccggact
     2221 caccctcacc tggtcggcca acggctccgt ggtgggcaac gccgagaaca tcaacgtgcc
     2281 gccacccgcc cagcccacct ggtacgtggc aagtgtaagc gacgcctgtg gtggctccgc
     2341 gagtgattcc acgctcgttt ccagcaccgt cccgcccatc tccatcaccg tgagccccga
     2401 cgtcatcatg ccttgtgatg gtacgggggc cactatcagc gtgaccgggg tttcgggagg
     2461 taccggcgcg atcggttatg tgtggacgag caatggcacg caggtcggca acgcccagga
     2521 tctcgcggtg ggccctgaac aggcccaata cacggtcacc gttac
//
LOCUS       DAOQOC010000356         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23252_length_2565_cov_2.908367, whole genome shotgun sequence.
ACCESSION   DAOQOC010000356 DAOQOC010000000
VERSION     DAOQOC010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23252_length_2565_cov_2.908367"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..>2565
                     /gene="pheT"
                     /locus_tag="PL106_06670"
     CDS             202..>2565
                     /gene="pheT"
                     /locus_tag="PL106_06670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNA32788.1"
                     /translation="MRISWNWLRTLIDTDLPPHEAADILTSTGLEVESVDPIEPVKGM
                     LAGVVVGQVVECTKHPDADRLRLCRVEVGTGDPLHIVCGAPNVATGQKVLVATIGTTL
                     YPVSGDPLTIKKSKIRGAESHGMICAEDELGLGAGHAGILVLDPSAVEGTPAAQQLGL
                     SSDFALEIGLTPNRADAMSHIGVARDLAAAITVRTGKKVSAKWPDVSAFVPGSGPGGI
                     QVEVRSTAACPRYAGITLTNVKVAASPAWLQDRLRSIGLKPINNVVDVTNFVQYELGQ
                     PLHAFDADQLKDDRIVVRMAAEGESFTTLDGRERALSAQDLVIADAGQPACIAGVFGG
                     LNSGVTDATTSIFLESACFDATTIRRTARRHGLNTDASFRFERGVDPEITVYALKRAA
                     LLLQDVAGATIASGITDLIARPAEWKQVQLPYHALNSLLGTRIGPEELRTVLEVLDCR
                     TVASDADSITVEVPPYRVDVTRPTDLIEEVVRIIGFDRVPLPERLMMPSVVRDEVTFD
                     GLQRQVAAHLAARGFHEVMTPSLVNGERTVRLNAATTASLVHLKNPLSTELDVMRPTL
                     LFGLLQSAAHNIARQQKSLRLFESGRIYGIRNDGTTFEQERIALLITGADRLETWRQQ
                     TGPASTVDIAAEVEDLLRRLNARTGPAEMTEHPLLHTAAHFATKRNTLVHWGQVRPDV
                     AKAFDLEQPAFYAELDLTACKEFLSGRSTTFVPVPRFPSVRRDLSLLLDSSVTFAQLE
                     RAARQSERKLLREVGLFDVYEGDKLPAGKKSYALSFILLDEDKTLTDE"
BASE COUNT          493 a          840 c          776 g          456 t
ORIGIN      
        1 agaccgggat cgagaggatg atgtatccca tcgacggacg aaactatcgc gttgtaaccc
       61 ttgcctaccc gggtgcgcga catcgaagat ccgctcctcc cccgtccgac ccggggcacc
      121 aaccgtgttc catgcgtccg gggtactttc gccagccgtt ttccccacca tccaccttcg
      181 ccggggctac ggcggatact gatgcgcatc tcttggaact ggctccgcac gctgatcgac
      241 accgaccttc ctccgcacga agcggccgat atcctcacca gcaccggtct ggaggtcgaa
      301 agcgtggatc cgatcgaacc ggtgaagggc atgctggccg gcgtggtggt gggccaggtg
      361 gtggaatgca cgaaacatcc ggatgcggac cggctgcgcc tgtgtcgtgt ggaggtcgga
      421 acgggcgacc cgctacacat cgtttgcgga gcacccaacg tggcgaccgg ccaaaaagtc
      481 ctcgtggcca ccatcggcac cacgttgtac cccgtctccg gcgatccact cacgatcaag
      541 aagtcgaaga tccgcggtgc tgagagccat ggcatgatct gcgccgagga tgaactcggc
      601 cttggtgcgg gccacgcggg catccttgta ctggacccct ccgccgtgga gggcacaccc
      661 gctgcgcaac agctcggcct ctcgtccgac ttcgcactgg agatcggcct cacgcccaac
      721 agggccgatg ccatgagcca catcggcgtg gcgcgtgacc tggccgccgc gatcaccgtc
      781 cgtacaggaa aaaaggtatc agctaaatgg cccgacgtgt ccgcgttcgt gccgggatcc
      841 ggtccgggtg gcatacaggt ggaggtccga tccaccgccg cctgtccccg ctatgccggc
      901 atcacgctca ccaacgtgaa ggtcgctgca tccccggcgt ggctgcagga ccgtttgaga
      961 agtatcggcc tgaagcccat caacaacgtg gtggacgtga ccaacttcgt acagtacgaa
     1021 ctggggcaac cgctgcacgc cttcgatgcg gaccagttga aggatgaccg catcgtcgta
     1081 cgcatggccg cggaaggtga gtcgttcacc acactcgatg gcagggaacg tgccctctcg
     1141 gcgcaggacc tggtgatcgc cgacgctgga cagcccgcgt gcatcgctgg agttttcggt
     1201 ggcttgaaca gcggtgtgac cgatgcgacc acgtcgatct tccttgaaag tgcctgcttc
     1261 gatgcgacca ccatccgccg gaccgcgcgc aggcatggcc tgaataccga tgcttcgttc
     1321 cgctttgaac gtggtgttga tcccgaaata accgtatacg cgctcaagcg tgccgcactg
     1381 ctgctgcagg acgtggccgg ggccaccatc gcatccggga tcaccgacct gatcgcccgc
     1441 cctgccgaat ggaagcaggt acagttgccg taccatgcgt tgaactcgct cctcggtacg
     1501 cgcatcggac cggaggaact gcgcaccgtg cttgaagtat tggactgtcg caccgtggcc
     1561 agcgatgccg acagcatcac cgttgaggtc ccgccttacc gcgtggatgt cacacgcccc
     1621 acggacctca tcgaggaggt ggtgcgcatc atcgggttcg accgggtgcc gctgcccgaa
     1681 cggttgatga tgccgtccgt ggtgcgcgat gaagtgacat tcgacggact gcaacgccag
     1741 gtcgcagctc atctggcagc gcgcggcttc cacgaggtga tgaccccgtc cctggtgaac
     1801 ggcgaacgca cggtacgcct gaacgccgcc accaccgcat cactcgtaca cctgaagaac
     1861 ccgttgagca ccgagttgga cgtgatgcgc cccaccctgc tcttcggcct gttgcagagc
     1921 gcggcgcaca acatcgcgcg ccaacaaaag agcctgcgcc tgttcgagtc gggcaggatc
     1981 tacggtatcc ggaacgatgg cactacgttc gagcaggaac gcatcgcgct gctcatcacc
     2041 ggtgccgaca ggcttgaaac atggcgccaa caaaccggac ctgcgagcac tgtggacatc
     2101 gctgcggagg tggaggatct tttacgtcgg ctcaacgcgc ggaccgggcc tgcggaaatg
     2161 accgagcacc ccctactgca cactgccgcg cacttcgcga caaagcgcaa cacccttgtt
     2221 cactgggggc aggtccggcc cgacgtggcc aaagccttcg atttggaaca gcctgctttc
     2281 tatgccgagt tggacttgac agcctgcaag gagttcttat cgggccgaag caccacgttc
     2341 gttccagtgc cccgtttccc ctccgtgcgt cgcgacctga gcctgctgtt ggacagctcc
     2401 gtgaccttcg cacaactgga acgcgcagcg cgccagagcg aacgcaagct cctgcgcgag
     2461 gtcggcctct tcgatgttta cgagggcgat aagctgcccg ccggcaagaa gagctatgcg
     2521 ctaagcttta tcctgctgga cgaggacaaa accctcaccg acgag
//
LOCUS       DAOQOC010000357         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23270_length_2564_cov_3.910721, whole genome shotgun sequence.
ACCESSION   DAOQOC010000357 DAOQOC010000000
VERSION     DAOQOC010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23270_length_2564_cov_3.910721"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..824
                     /locus_tag="PL106_06675"
     CDS             141..824
                     /locus_tag="PL106_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32789.1"
                     /translation="MNNDLLIDRLKNARSTLDPGDHTTSFATPPTGTYQPEAAHMART
                     IKLALLALTCGLLNHANAYNLRVRGVVTEYLTGDLLTDALVRVYKDGVKQQAEQTGVL
                     GSYSFTLDNNAKYVLRFSAPGHQTKCFTIDTHGLEWEGEGGMKDLYVEMTLFQQLSDM
                     DLSFFDMPMGMAFFEPATGLVRWDTDYDRRIRPEVQSLMIGYTRRMTAMANATSSIPK
                     RRTQNDAAR"
     gene            914..2302
                     /locus_tag="PL106_06680"
     CDS             914..2302
                     /locus_tag="PL106_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNA32790.1"
                     /translation="MKYFLWFCRIVVGGTFIVSGLVKANDPLGFSYKLEEYFAESALN
                     LPFLEPASLFLAILACLAEVVLGFAVIVGGRMKLATACILWLTLFFGWLTAYTATCDP
                     QGKYEVVVNGVKELRDVTCVTDCGCFGDAMKGSLGRSLTPWESFYKDLILLIFVLPLF
                     FVSWFRKPRPSEAAGEGGPGIAFNTGGDDKVLLPGALILVALWCWVFGWAFFLWFTLT
                     GLAGYLVIKRLAQGVKAEWLTALWITVFSLGFIWYCYAHLPLKDYRAYAVGKSISEQK
                     ALAKPPVNEIFLVYKDKSTGEEREYPGNNYPWNDSVWVATHEFVKQRVNVIDPGVPSQ
                     VQDFRLTDNDGVDITDDVLAEPTPVLLIVVKTIGEANTSCLPGIKTLTDAASTAGWYV
                     YGVTATGHDETEAFRHEHQLAFDFVTCDEITLKTMIRSNPGVVLLKEGRVMGQWHCND
                     VPSLEEAKKSVE"
BASE COUNT          500 a          772 c          792 g          500 t
ORIGIN      
        1 attcctgtgc ggatgccgaa aggacgatcg ccacggtggg caggaggagc atggggcgga
       61 gcatggccgg gggattaacg gcgaaagatg gcacaaagct cggcaacttg acctgagaag
      121 gatcagccgg gagatgcgcc atgaacaatg acctgttgat cgatcgcttg aaaaatgccc
      181 gatcgacctt ggatcccggg gaccacacga cttcatttgc caccccgcca acaggcactt
      241 accagcccga ggcggcgcac atggcacgaa cgatcaagct ggcactcctt gccctcacct
      301 gcggtcttct gaaccacgcg aacgcctaca acttacgggt tcgtggcgtt gttaccgaat
      361 acctcaccgg ggatctcctc accgatgcgt tggtgcgcgt gtacaaggac ggcgtgaaac
      421 aacaagccga gcagaccggt gtgctgggga gttactcctt caccttggac aacaacgcga
      481 agtacgtgct ccgcttcagt gcgccggggc accagaccaa gtgtttcact atcgatacgc
      541 acgggctgga gtgggaaggg gaaggaggga tgaaggacct ctacgtggag atgaccctgt
      601 tccagcaact gtcggacatg gacctgtcgt tcttcgatat gcccatgggc atggcctttt
      661 ttgagccggc cactggcctg gtgcgctggg acacggatta cgaccggcgc atccgtccgg
      721 aggtgcagag cctgatgatc ggctacacgc ggcgcatgac cgccatggcc aatgccacct
      781 cgagtatccc caagcgccgc acgcagaacg acgcggcccg ttaatgatcc gttgatcccg
      841 gatccggaac ggggtcgcag ggtattttcg cggccgttgt catgggcaca tacgcccgtt
      901 cacacccggt cgcatgaagt attttctctg gttctgtcgc attgttgttg gtggcacctt
      961 catcgtgagc ggcctggtga aggccaacga cccgctcggg ttcagctaca agctggagga
     1021 gtatttcgcc gagagcgcgc tcaacctgcc ctttctggag ccggcttcgc tctttttggc
     1081 catcctggcc tgcctggccg aagtggtgtt gggcttcgcc gtgatcgtgg gcgggcgaat
     1141 gaagttggca acggcctgca tcctgtggct caccttgttc ttcggctggc tcaccgccta
     1201 cactgccacc tgcgatccgc aaggcaaata cgaagtggtg gtgaacggcg tgaaggaact
     1261 gcgcgacgtc acctgcgtca ccgattgcgg ctgcttcggc gatgcgatga agggctccct
     1321 gggccgcagc ctcacaccct gggagagctt ttacaaggat ctcatcctgc tgatcttcgt
     1381 actgccactc ttctttgtaa gctggttccg caaaccgcgc ccgtccgaag ccgctggcga
     1441 aggcgggccg ggtatcgcat tcaacaccgg tggtgatgac aaggtgctgc tgcctggcgc
     1501 actgatcctg gttgcgctct ggtgctgggt gttcggctgg gccttcttcc tgtggttcac
     1561 cctgacgggc ttggccggct atctcgtcat caagcggctg gcccaagggg tgaaggccga
     1621 atggctcacc gccctctgga tcaccgtgtt ttcactggga ttcatctggt actgttacgc
     1681 acacctgccc ctgaaggact accgggccta cgccgtgggc aagagcatca gcgagcagaa
     1741 agccttggcc aaaccgccgg tgaacgagat cttcttggtg tacaaggaca agagcaccgg
     1801 cgaggagaga gagtaccccg ggaataacta cccttggaac gatagcgtgt gggtggccac
     1861 gcacgagttc gtgaaacagc gtgtgaacgt gatcgacccc ggcgtgccct cgcaggtgca
     1921 ggacttccgc ctcacggaca acgacggggt ggacatcacc gacgacgtgc tggccgagcc
     1981 cacaccggtg ctgctgatcg tggtgaaaac gatcggcgag gcgaacacat cctgcctgcc
     2041 ggggatcaag accttgaccg atgcggcttc caccgccggt tggtacgtgt atggcgttac
     2101 ggccaccggt catgatgaga cggaggcctt ccgccacgag caccagttgg ccttcgactt
     2161 cgtcacctgc gacgagatca ccctgaagac catgatccgc agcaacccgg gggtggtcct
     2221 tctgaaggaa ggccgcgtga tgggccaatg gcactgcaac gacgtgccct cgcttgaaga
     2281 ggcgaagaag tcggtggagt gagcgtccgc agcacgccta cgaccacttg accgggtggc
     2341 ccgtacggcc aaccatcggg accttcatac gtctatcttg gcgaacagcc atgcgcacct
     2401 gccgcagtat cgcctcggtc atcggagtcc tggtcgtcgc ttcggtgatc gccagtggac
     2461 caggaacacc gcgctacaag cggcttttcc gcacgtggaa ggacaatccg cacgccagcc
     2521 acgccttttt cacgcccagt gccatcggac tgccgcgggg caat
//
LOCUS       DAOQOC010000358         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23287_length_2563_cov_3.045853, whole genome shotgun sequence.
ACCESSION   DAOQOC010000358 DAOQOC010000000
VERSION     DAOQOC010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23287_length_2563_cov_3.045853"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="PL106_06685"
     CDS             complement(<1..2086)
                     /locus_tag="PL106_06685"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32791.1"
                     /translation="MHPAYRGMRTVVRHLTLCASSAILVLAACKREQAAIRPDAAFTP
                     YIPAFTAGHIPARGPLLIRIADGQSWKDTSAAALQDLFDLDPGAKGTVTWYDANTLAF
                     RPDDRLDQNTVYTVRFHLGNVIEVPPALKTFMFQVTTFPQSISLKVDDMGPVSPGNPN
                     WQRVHVGLHTSDLAEPSDLEKCVTAEQDGRALTLLWEHAPNGSYHRAVADSVMRGNKV
                     GEVIFRWNNGGIGGEGQGEERFSIPALGDLHLIGQATFSEGEQYAALLFSDPLDPAQD
                     LSGLAGISGTENVRLAIEGNKLLLYPAERISGTRSAFVAAGLRTGSQNVLGKDIIVPD
                     LEFEELKPNVRISGNGVILPSTDGLYFPFEAVNLNAVDVRIVRIYTDNVPQFLQVNAL
                     DGERELARTGRLLVKKTIPLDNADGNVTGRWNTYHLDLAQLIKAEPGAIYRISIGFRK
                     SQSTYPCGGGEAPPALAEMNEDTGGDQEEWDNPDQDYWYYDEGGYYEDEYNYNEREDP
                     CSESYFRGKASVVSRNILASDIGLIAKRGNDGSLLVAASDLRTAKPMSGVRIEVLDLQ
                     RQSLGAPVTNSEGLITLPTSKHRPFLVMASKGAMRGYLKLDDGGALNVSSFDVGGQEV
                     DKGLKGFLYGERGVWRPGDSLYLGFILQDAGNKLPKDYPVVFELSDPQGRLDQRLVRT
                     GGTGGVYAFRCAT"
     gene            2235..>2563
                     /locus_tag="PL106_06690"
     CDS             2235..>2563
                     /locus_tag="PL106_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32792.1"
                     /translation="MRTTTSIMAWAVLLPALAQQSNTEEHLADLAAHARSTSTVPSHR
                     WSGAGGRFGPGEMIDHMPLRAIVWEFGDGGLGSILPPQPWSPEEGGLSRDEDMAPWRE
                     YASGSPCV"
BASE COUNT          456 a          759 c          838 g          510 t
ORIGIN      
        1 atgtggcaca gcggaaggcg tacacgcccc cggtacctcc ggtgcgaacg aggcgctggt
       61 ccaagcgacc ttgggggtcg ctgagttcaa atactaccgg ataatccttc ggcagtttgt
      121 tcccggcgtc ctgcaggatg aagccgaggt acaatgaatc acccgggcgc cacaccccac
      181 gttcgccgta caggaagcct ttcaggccct tgtccacttc ctggcccccc acatcgaagg
      241 aactgacgtt gagggccccg ccatcatcca gcttgaggta tccccgcatg gcgcctttgc
      301 tggccattac aaggaacggg cggtgcttgc tggtgggcag ggtgatcagt ccctcgctgt
      361 tcgtgaccgg cgcaccaagg ctttgccgct ggaggtcgag cacttcgatc cgcacaccgc
      421 tcatgggctt ggccgtgcgc aggtcgctgg cggccaccaa caaggaccca tcgttaccgc
      481 gtttggcgat caggccgatg tccgaagcga ggatgttgcg ggagaccacg ctggccttgc
      541 ctcggaagta gctctctgaa cacggatcct cccgctcgtt gtaattgtac tcatcctcgt
      601 agtagccgcc ttcatcgtag taccagtagt cctggtcggg gttgtcccac tcctcctgat
      661 cgccaccggt gtcctcgttc atttctgcga gcgcgggggg ggcttcaccg ccgccacagg
      721 gataggtgga ttgcgacttc cggaagccga tgctgatgcg atagatggct cccggctcgg
      781 ccttgatgag ctgcgcaagg tcgaggtgat aggtattcca gcgtccggtc acgttgccat
      841 ccgcgttgtc cagtggaatg gtctttttga ccagcaggcg ccccgtacgt gccagttccc
      901 gctcgccatc cagggcgttc acctgcagga attgtggaac attatcggta tagatccgaa
      961 cgatgcggac atccacggcg ttcaggttca ccgcttcaaa ggggaagtag agaccatcgg
     1021 tcgatggcag gatgacgccg ttgcccgaga tgcgcacatt gggcttcaac tcctcgaatt
     1081 ccaggtcggg cacgatgatg tccttgccca gcacgttctg gctgcccgtg cgcaacccgg
     1141 cggccacgaa tgccgaacgt gtgccgctga tccgttccgc cgggtagagc aggagcttgt
     1201 tgccctcgat ggcgaggcgc acgttctccg taccgctgat gcccgcgagt ccggagagat
     1261 cctgtgcggg atcgagcggg tcgctgaaga gcagcgcggc gtattgttcc ccctcgctga
     1321 aggtcgcctg accgatcagg tgcagatcac cgagcgcagg tatgctgaac cgctcttcgc
     1381 cctgaccctc accgccgata ccgccattgt tccagcggaa gatgacctcg ccgaccttgt
     1441 tcccgcgcat cacgctgtcg gccacggcac ggtggtagct gccgttgggt gcgtgttccc
     1501 agagcagggt gagcgcccga ccatcctgtt cggcggtcac gcacttctcc aggtccgatg
     1561 gctccgcgag atcgctggtg tgcagaccga catgcacgcg ctgccagttc gggttgccgg
     1621 gcgaaacggg ccccatgtcg tccactttca agctgatgga ttgaggaaag gtggtgacct
     1681 ggaacatgaa cgtcttgagc gcgggcggga cctcgatcac gttgccgagg tggaagcgca
     1741 cggtgtacac ggtgttctgg tccaagcgat catctggccg gaaagcgagg gtgttggcgt
     1801 cgtaccaggt aacggtgcct ttcgctccgg gatccaggtc gaagaggtcc tggagcgcgg
     1861 ccgcggatgt gtctttccag gactggccat cagcgatgcg gatgaggagg ggaccacgtg
     1921 ccggtatgtg cccggcggta aaggccggta tgtacggcgt gaatgcggca tcgggccgga
     1981 tggcggcctg ttcgcgtttg caggcggcaa gaaccaggat cgcactggag gcgcagaggg
     2041 tgaggtggcg cacaacggtg cgcattcccc ggtaggcagg gtgcatgatc aaggaaggaa
     2101 gagtgggagg caaggtagga tcccggtgca tgtgcaacgt aatcgaggtg aggggtattg
     2161 caatatggcg aaggtgaatg gaaccattcc cgggctcaca gcgttggaag gcatcgccgg
     2221 agaaccggtc atccatgcgc acgaccacct ctatcatggc ctgggccgtt ctgctgcccg
     2281 cattggccca gcaaagcaac acggaggagc acttggcgga cctggccgct catgctcgtt
     2341 cgacctccac ggtacccagc caccgatggt ccggcgctgg tggtcgcttc ggcccgggag
     2401 agatgatcga tcacatgcct ttgcgtgcca tcgtttggga attcggcgat ggtggccttg
     2461 gcagcatcct gccaccgcaa ccttggagcc cggaggaggg cgggttatcg cgtgacgagg
     2521 atatggcgcc ctggcgtgaa tatgcgtccg gttcaccctg tgt
//
LOCUS       DAOQOC010000359         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23575_length_2546_cov_3.410277, whole genome shotgun sequence.
ACCESSION   DAOQOC010000359 DAOQOC010000000
VERSION     DAOQOC010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23575_length_2546_cov_3.410277"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PL106_06695"
     CDS             <1..404
                     /locus_tag="PL106_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32793.1"
                     /translation="RLEIIKKLNIVVAIVLALPHGVMGLVVGEAVVSVVNMLVDTWYT
                     RGLLGWHPMEQIGDVLPSLLIAIIAGVVVLMAKMILTVSGPLALAALASIGIIAYVVP
                     HVLLGTREWRTITGTLLPKALATVTHDRNTR"
     gene            401..1345
                     /locus_tag="PL106_06700"
     CDS             401..1345
                     /locus_tag="PL106_06700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA32794.1"
                     /translation="MNDGQEPHVRAAHTTGPSVDVLMLARNVADVIGQAVDGVLAQRC
                     PFPVRLVIAEDGSTDRTPAICEALAREHGGRIAYRPGGKNIGIAARTVEALSRCSARY
                     VAICDSDDIWTDPNKLAKQVGFLETHPDHAFSYTDVDIITREGMHVDADGYQGIRSRY
                     AGGWLFAQLVQGNFVNNSTTVARRDLLEGLRPNACRDELIGDHVRWIQLAMRGKAHFL
                     PARTTAYRQGGVTGQTAIQQLNRKVMLGMLPGLLREHASMRTPTTAADRAILLRKTAG
                     TMLRTGHLSLIPLLFRYLPAVFPSRSPSGGGPVDSHDT"
     gene            1342..2121
                     /gene="rfbF"
                     /locus_tag="PL106_06705"
     CDS             1342..2121
                     /gene="rfbF"
                     /locus_tag="PL106_06705"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNA32795.1"
                     /translation="MKVLILAGGYGSRLSEETGPRPKPMVEIGGRPILWHIMKSYSHH
                     GFNEFIILCGHLGHVIKEYFANYHLRSSDVTFDLAGNTTRIHRNPSEPWSVTLLDTGP
                     DTMTGGRVLRARPYLAQGEPFLLTYGDGVGNVDIPSLIAFHKAHGRTMTMTSAQPEGR
                     FGALDIGTDGRVGRFVEKPKGDGAWINAGFFVCEHRMLDQLTEGDATVLERAPLERMA
                     AEGELYAYKHHGFWRPMDTLRDRMALDELWARGEAKWKIWP"
     gene            2118..>2546
                     /locus_tag="PL106_06710"
     CDS             2118..>2546
                     /locus_tag="PL106_06710"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA32796.1"
                     /translation="MRIEEAFRGRRVLLTGHTGFKGGWLAIWLDMLGAEVVGVALPPD
                     QPDGIYQASGIGGRIRDIRQDIRDGEALEACFAEHAPEVVLHLAAQALVRESYRTPAA
                     TFATNTMGTVNVLEAVRHTPSVKALVVATTDKCYENTGITS"
BASE COUNT          471 a          839 c          813 g          423 t
ORIGIN      
        1 ttcgcctcga gatcatcaag aagctgaaca tcgttgtggc catcgtcctg gcgctgccgc
       61 acggcgtcat gggcctggtc gtgggcgaag cggtcgtgtc ggtggtgaac atgttggtgg
      121 acacctggta cacacggggc ctgttgggct ggcaccccat ggaacagatc ggcgacgtgt
      181 tgccatccct gctgatcgcg atcatcgccg gggtcgtggt gctcatggcg aaaatgatac
      241 tgaccgtcag tggcccgctc gcccttgccg cgctggcatc catcggtatc atcgcgtacg
      301 tggtaccgca cgtgctgctg ggcacgcgcg aatggcgcac gatcacgggc accttgcttc
      361 ccaaggccct cgccacggta acgcacgacc ggaacacacg atgaacgacg gccaggagcc
      421 ccatgttcgt gcggcgcata ccaccgggcc ttcggtggat gtgctcatgc tcgcccgcaa
      481 cgtggcggac gtgatcgggc aggccgtaga tggtgtgctc gcgcaacggt gtccattccc
      541 cgtgcggctg gtgatagccg aggacgggtc caccgaccgt acgcccgcga tctgcgaagc
      601 cctggcacgc gagcatggcg ggcggatcgc ataccggccc ggcggcaaga acatcgggat
      661 cgccgcccgc accgtggaag ccctgagccg ttgcagcgcc cgctacgtgg ccatctgcga
      721 cagtgacgac atctggaccg acccgaacaa gctcgccaag caggtcggct tcctggaaac
      781 gcatccggac catgccttct cgtacaccga tgtggacatc atcacccgcg aaggcatgca
      841 cgtggatgcg gacggctacc agggcatccg atcgcgctat gcgggcggtt ggctgttcgc
      901 gcagctggtc cagggcaact tcgtgaacaa ctcgaccact gtggccaggc gggacctgct
      961 ggaaggtctg cggcccaacg catgccggga cgagttgatc ggcgaccacg tgcgctggat
     1021 ccaactggcc atgcgcggca aagcccactt cctgcccgca cgcaccacag cgtaccgtca
     1081 gggcggggtc accggtcaga cggccatcca gcaactcaac cgcaaggtga tgctcggcat
     1141 gctgccggga ctcctgcgtg aacatgcctc gatgagaaca cccaccactg cggccgaccg
     1201 tgccatcctg ctgcgaaaaa cggcgggaac catgctacgc acgggccacc tttcgctcat
     1261 tcccctcctc ttccggtatc ttcccgccgt gttcccttcg cgttcgccca gcggcggcgg
     1321 accggtagac agccacgaca catgaaggtc ctgatccttg ccggcgggta tggttcgcga
     1381 ttgagcgaag aaaccggccc gcgccccaag cccatggtcg agatcggtgg acgaccgatc
     1441 ctctggcaca tcatgaagtc gtattcccac cacggcttca acgagttcat catcctgtgc
     1501 gggcacttgg gccatgtgat caaggagtac ttcgccaact accacttgcg ctcctccgat
     1561 gtcaccttcg acctggccgg gaacaccacg cggatacacc gcaatcccag cgaaccgtgg
     1621 tcagtaacgt tgctggatac ggggccggac accatgaccg gcgggcgcgt gttgcgtgcc
     1681 cggccgtacc tggcccaagg agagcctttt ctgctcacgt acggcgacgg cgtggggaac
     1741 gtggacatac cgtcgctcat cgccttccac aaggcccacg gccgtaccat gacgatgacc
     1801 tcagcgcagc ccgagggccg cttcggcgcg ctggacatcg ggacggacgg tcgggtcggg
     1861 cgcttcgtgg agaaacccaa gggcgacggt gcatggatca acgcaggttt cttcgtgtgc
     1921 gaacaccgca tgctcgacca gctcaccgaa ggtgacgcca ccgtgctcga acgcgcaccg
     1981 ctcgaacgca tggcggccga aggcgaactg tacgcataca agcatcacgg cttctggcga
     2041 cccatggaca ccttgcgcga ccgcatggcc ctggacgaat tgtgggcacg tggcgaggcc
     2101 aaatggaaga tctggccgtg aggatcgagg aggccttccg cgggcgacgg gtgctgctca
     2161 ccggccacac cggcttcaaa ggcggctggc tggccatctg gctcgacatg ctgggggccg
     2221 aggttgtcgg cgtggcgctc ccacccgatc agcccgatgg aatttaccaa gcaagtggta
     2281 tcggtggccg tatccgggac atccgccagg acatccgcga cggggaggcg ctggaggcct
     2341 gcttcgcgga gcacgcgccc gaggtggtgc tgcacctggc cgcgcaggcc ctcgttcgcg
     2401 agagctaccg cacgcctgcg gccacgttcg caacgaacac gatgggcacg gtgaacgtgc
     2461 tggaggccgt gcggcacacg ccatccgtga aggcgctcgt ggtggcgacc accgataaat
     2521 gctacgagaa cacaggcatc acatcc
//
LOCUS       DAOQOC010000360         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23593_length_2545_cov_3.289558, whole genome shotgun sequence.
ACCESSION   DAOQOC010000360 DAOQOC010000000
VERSION     DAOQOC010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23593_length_2545_cov_3.289558"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PL106_06715"
     CDS             complement(<1..311)
                     /locus_tag="PL106_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32797.1"
                     /translation="MVAFILLALLLAPLLVVAYVLNFSPRVGANPSGERLQRIQRQPN
                     HKDGKLHNLIPTDMRMSPRTMFKVMWWMLRGGEGREPERPLPVFAFDREAWARVPTDE
                     VA"
     gene            complement(521..1849)
                     /locus_tag="PL106_06720"
     CDS             complement(521..1849)
                     /locus_tag="PL106_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32798.1"
                     /translation="MKTSRSFSYALAGLCAVPAALLSFHEAPKPCCMDHPEHAQPAGC
                     VFKTVYEGEGLDPAFVLKTPSEVLASENRAYDWMVAAQNKDGGFAAGQHAHQEVMDPH
                     AVPSDPATTAMVGMAMLRAGSTLVEGRFSEPLKRATEFLLKTVEAAPEDALNITPLTG
                     TQIQVKLGQNIDVALTAQYLSNLMGRMPCNDPKHDRWLQALNTCTRKIQKAQSGDGST
                     AGSGWAGVLQSSFATNALESAKHVGAAVSDTALAKAREWQQGNFNASTGTVATDAAAG
                     VTLYAVSSSTRSSAKQAREANEVLSKAKKEGKVEDSAPVNVESLMDAGMNRDDAEKAN
                     TAYKVYEAAKVQAQADNVISGFGNNGGEEFLSFLQTGESLIINKDESWKNWYGSTTAR
                     LVEIQNPDGSWNGHHCITSPVFCTATSLLILSVNNDIEQLVAQGATVKRK"
     gene            1970..>2545
                     /locus_tag="PL106_06725"
     CDS             1970..>2545
                     /locus_tag="PL106_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HNA32799.1"
                     /translation="MGIRMSNKAVRGIISASFLVPCMLVHAQQPADRNRFDSLYVKDY
                     SHILTLRAYISTKYNSLQLTAGNGAKDLLFRPNNKVNFGVGASYRSLTLNIGVGIPGV
                     NKDQDKYGETRYLDAQANVYTKHWATNIFLQRFKGYYLDTYTKDELGWQQASFYPTRP
                     DLVQFNLGASTVHIFNNDRFSYRAAFNQDAWQ"
BASE COUNT          462 a          836 c          732 g          515 t
ORIGIN      
        1 gccacttcat ccgtgggaac acgtgcccag gcttcgcgat cgaacgcaaa aaccggtagc
       61 ggccgttcag gctcgcgccc ttccccgccc cgcagcatcc accacatcac cttgaacatg
      121 gtgcgcggtg acatgcgcat gtcggtgggg atcaggttgt gcagcttgcc gtccttgtgg
      181 ttcggctggc gttgtatgcg ttgcaggcgt tcgccggacg ggtttgcgcc cacgcgcggc
      241 gagaagttga gcacgtaggc caccaccagc aatggagcga gcagcagtgc gagaagtatg
      301 aacgcgacca tgaccgatgc gcagcgaaga tccaacaacc gcgctgccga ccacctccag
      361 cgataacgcc ctgtgtccgt tcatccctcg ctgtgccggt atacaccgtc agcgggatcc
      421 atccctgccg cgctccacca cgaaggcgat caggaaggtc ccgctgacga aaacacgagc
      481 gtacgaggat gcgcgatccg ccgctaccgc acctttgcgc tcacttccgc ttcaccgtgg
      541 ccccttgcgc caccagctgc tcaatgtcgt tgttcacgct gaggatcagc agcgatgtgg
      601 ccgtgcagaa caccggactg gtgatgcagt ggtggccgtt ccagctaccg tccgggttct
      661 ggatctcgac gagtcgtgcg gtggtgctgc cgtaccagtt cttccagctc tcgtccttgt
      721 tgatgatgag cgactcgccc gtttgcagga aactgaggaa ctcctcgccg ccgttgttcc
      781 cgaaaccact gatcacgtta tcggcctggg cctgcacctt ggcggcctcg tataccttat
      841 atgcagtatt tgccttctcc gcgtcgtccc gattcatgcc cgcgtccatc aggctctcca
      901 cgttcaccgg ggccgagtcc tccaccttgc cttccttctt ggccttgctg agcacctcgt
      961 tcgcttcgcg ggcctgcttg gcactgctgc gtgtactgct gctcacggca tacagcgtca
     1021 cacccgcggc agcatcggtc gccacggtac cggtgctcgc gttgaagttc ccctgctgcc
     1081 attcgcgcgc cttggccagg gccgtatcgc tgacggcggc gcccacatgc ttcgcgctct
     1141 ccagcgcgtt ggtcgcgaag ctgctctgca gcacgccggc ccagccgctg cctgcggtgc
     1201 tgccatcgcc gctctgtgcc ttctggatct tgcgcgtgca tgtgttcagc gcttgcagcc
     1261 agcgatcgtg cttcggatcg ttgcacggca tgcggcccat gaggttgctc aggtattgcg
     1321 ccgtgagggc cacgtcgatg ttctgcccca gcttcacctg gatctgcgtg ccggtgaggg
     1381 gggtgatgtt cagcgcatcc tctggcgcgg cctccacggt cttcagcagg aattcggtcg
     1441 cacgcttcaa tggctcgctg aaccggccct ccaccagtgt gctgcccgcg cgcaacatcg
     1501 ccatgcccac catggcggtg gtggccggat cgctcggaac ggcgtgcggg tccattacct
     1561 cctggtgcgc gtgctggccg gccgcgaaac cgccgtcctt gttctgcgcc gcaaccatcc
     1621 aatcgtaggc acggttctcg gaagccagca cctcggaagg cgttttcagc acaaacgcag
     1681 ggtccaagcc ttccccttca taaacggtct tgaacacaca acccgcaggc tgtgcatgtt
     1741 caggatggtc catgcaacag ggcttgggtg cctcatggaa ggaaagcagt gccgccggca
     1801 cggcacagag gccggcaagt gcgtaggaaa aggaccggga cgttttcatg gtgcggttga
     1861 tttttttgaa gtgttgggac cgtgttgccg tacacggcaa tgaccatgag gttcaccgca
     1921 tgaaaagtca caccgcgctt tacaagcaag cgcaacgaac tttgcgccca tgggaattcg
     1981 catgtccaac aaggcggtcc gcggcatcat cagtgcctcg ttcctcgtgc cttgcatgtt
     2041 agtgcatgca cagcagcctg cggacaggaa ccgctttgat tccctctatg tgaaggatta
     2101 ctcgcacatc ctcacgctcc gtgcgtacat cagcaccaag tacaacagcc tgcagctcac
     2161 ggccggcaat ggggccaaag acctgttgtt ccgccccaac aacaaggtca acttcggtgt
     2221 gggcgccagc taccgctcac tgacgctgaa catcggcgtg ggcatccccg gcgtgaacaa
     2281 ggaccaggac aagtacgggg aaacgcgcta cttggacgca caggccaacg tgtacaccaa
     2341 gcactgggcc accaacattt tcctgcagcg cttcaagggt tattacctcg acacgtacac
     2401 caaggatgag ctggggtggc aacaagcctc gttctacccc acacgcccgg acctggtgca
     2461 gttcaacctt ggcgccagca cggtgcacat cttcaacaac gaccggttca gctaccgcgc
     2521 agccttcaac caggacgcat ggcag
//
LOCUS       DAOQOC010000361         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23792_length_2533_cov_3.787732, whole genome shotgun sequence.
ACCESSION   DAOQOC010000361 DAOQOC010000000
VERSION     DAOQOC010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23792_length_2533_cov_3.787732"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PL106_06730"
     CDS             <1..243
                     /locus_tag="PL106_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32800.1"
                     /translation="WEDVGSYGDSMYNQAVLMNMKDLSLRHLIGFPMISAKYELLDRS
                     KNPGNLYNFKLNYWTGNVAPILKQASYTGRFTVNGI"
     gene            310..1248
                     /locus_tag="PL106_06735"
     CDS             310..1248
                     /locus_tag="PL106_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32801.1"
                     /translation="MAQQQAYTKEKYLSELKEWGVPVPDGAKYPDIRALWEETVEARE
                     ARESRAVPADVEHIAGGSISPEVLAAAVEAYLAKRPAQAPASGLSKEDLREMLQAVKD
                     GPVNPLGQLRTERIAPGDRIAPVTFFCPAPRYYITEKWSGDYAEALPIGMGGILKFRQ
                     EFSYTVRSGDVHQTRFLSTLVVSSLTLYKWLTGEDLQGNKVGEPHEFYNRMFFRNANR
                     AIERSDNSLRAQIFDKHLFGASGLRFDQLVQRAQELGLPTNVDWTNQQYRAAIADRLT
                     DNELNAMAERAKEQARGYAAEKLLLKNEGVTVQAPV"
     gene            1258..2115
                     /locus_tag="PL106_06740"
     CDS             1258..2115
                     /locus_tag="PL106_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32802.1"
                     /translation="MAFTAQEVVDAASGQVDAEASQRYLPEQDWLPAINATYRRAVTA
                     CNFALANNKGSEEQTAELKVDLLFRTNGVGAIDIGGARNAEVPAQGNGIKPWSVLAVY
                     AEPERFALVPQVPVQPLPEPPNSSLTYYYAGGSSRPALSKFPVQRITAEQLAVAANNI
                     MLPGNERLALDRNNNPGKMRSYAYAIAGDQHSINDSSPGDMPGLTLLMRPIALSRVKW
                     FWVSYLRAPVTLTAMTGPNTGTVHLPLSMLQTFAEWTLQYLSVKQGDGTTLYGVADRD
                     AAQLFQFSV"
     gene            2115..>2533
                     /locus_tag="PL106_06745"
     CDS             2115..>2533
                     /locus_tag="PL106_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32803.1"
                     /translation="MATIRTIADEVAVALGFTHDDARLSFMALAYHVKLAVDKLRGRA
                     LAKDMVGDQRRAEDMLEVFAVDVVANTANDTVAWNCLYFDLPHDLYDLPFGGGLAWVR
                     YHRPSLPLNCPPGIAGAEFTHTTLGDLGRLYRLAYEVP"
BASE COUNT          511 a          824 c          806 g          392 t
ORIGIN      
        1 tgggaggacg tgggcagcta cggcgacagc atgtacaacc aggccgtgct gatgaacatg
       61 aaggatctga gcctgcgcca cctcatcggc ttcccgatga tcagcgcgaa gtatgaactg
      121 ttggaccgga gcaagaaccc cggcaacctg tacaacttca agctgaacta ctggaccggc
      181 aacgtggcgc cgatcctcaa acaagcctcc tacacgggac gcttcaccgt gaacggcatc
      241 tgatccgtga cctgacaaca ggcgagcccc gggcgaccgt gcccggggca tcgctccaaa
      301 accccgcaca tggcccaaca gcaagcctac accaaggaga agtatctgag cgaactgaag
      361 gaatggggcg ttccagtgcc cgatggcgcg aagtaccccg acatccgcgc gctctgggaa
      421 gaaacggtcg aggcccggga ggcccgcgaa agcagggcgg tccccgcaga cgttgaacac
      481 atcgccggtg gcagcatttc gcccgaggtg ctggccgcag cggtggaagc ctatttggcc
      541 aagcgcccgg cacaagcccc ggcttccggt ctttccaagg aggatctgcg cgagatgttg
      601 caggccgtga aggatgggcc ggtgaacccg ctggggcaac tgcgcaccga gcgcatcgcc
      661 cccggcgacc gcatcgctcc cgtcaccttc ttctgcccgg ccccgcgcta ctacatcacc
      721 gagaagtgga gcggcgacta cgccgaggcg cttcctatcg gcatgggggg gatcctgaag
      781 ttccggcagg agttctccta cacggtacgc agcggcgatg tgcaccagac gcgcttcctg
      841 agcacgctgg tggtgagcag cctcacgctg tacaagtggc tcacagggga agacctgcaa
      901 ggcaacaagg tgggcgagcc gcacgagttc tacaaccgga tgttcttccg caacgccaac
      961 cgcgccatcg agcgcagcga taacagcctg cgtgcgcaga tcttcgacaa gcacctcttc
     1021 ggggcgtcgg gcctgcgctt cgaccaactg gtgcagcggg cgcaggaatt gggcctgccc
     1081 accaatgtgg actggaccaa ccagcagtac cgcgccgcca tcgccgaccg cctgacggac
     1141 aacgaactga acgccatggc cgaacgcgcc aaggagcagg cgcgcgggta tgccgccgaa
     1201 aaactgctgc tgaagaacga gggcgtcacg gtacaagcac ctgtctgatc cacacccatg
     1261 gcattcaccg cgcaggaggt cgttgatgcc gcctcggggc aggtggatgc tgaggcttcg
     1321 cagcgctacc tgcccgaaca ggactggctg cccgccatca acgctaccta ccggcgggca
     1381 gtgacggcct gcaacttcgc cctggccaac aacaagggtt ctgaggagca gaccgccgaa
     1441 ctgaaggtgg acctgctttt ccgcaccaat ggcgtggggg ccatcgacat cggcggtgcc
     1501 cgcaacgcgg aggtccccgc gcaggggaac gggatcaagc cgtggagtgt gttggcggtg
     1561 tacgcggagc ccgaacgttt cgcgctggtg ccgcaggtgc ccgtgcagcc gctgccagag
     1621 ccgcccaact cgtcactcac ctactactac gccggaggga gcagccgccc ggcgttatcg
     1681 aagttccccg tgcagcgcat cacggccgaa caactggcgg tggcggccaa caacatcatg
     1741 ctgccgggca atgaacgcct cgcgctggac cgcaacaaca accccggcaa gatgcgcagc
     1801 tacgcctacg ccatcgcggg ggaccagcac tcgatcaacg acagcagccc gggcgacatg
     1861 ccgggcctca cgttactcat gcgccccatc gcgctgagcc gcgtgaagtg gttctgggta
     1921 agctatctgc gggcgccggt gacactgacg gccatgacag gccccaacac cggcacggta
     1981 cacctgccat tgagcatgct acaaacgttt gccgaatgga cgctgcaata cctgagtgtg
     2041 aagcagggtg acggtaccac cctctatggc gtggccgaca gggatgccgc gcaactgttc
     2101 caattctccg tgtgatggcc acgatccgca caatagccga cgaggtggcc gtagcgctgg
     2161 gcttcacgca tgatgatgcg cgcctgtcct tcatggcgct ggcctaccat gtgaagttgg
     2221 ccgttgacaa gctgcgcggg cgggcgctgg ccaaggacat ggtgggcgac cagcgccgtg
     2281 ccgaggacat gctggaggtc ttcgccgtgg atgtggtggc gaacacggcc aacgataccg
     2341 tagcgtggaa ctgcctgtac ttcgacctgc cgcacgacct gtacgacctg cccttcgggg
     2401 gcggcctcgc atgggtgcgc taccaccgtc cgtcgttgcc gctgaactgc ccgccgggaa
     2461 ttgccggagc cgagttcacg cacaccacgc tcggtgatct ggggaggttg taccggctgg
     2521 catacgaagt gcc
//
LOCUS       DAOQOC010000362         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23810_length_2532_cov_3.968107, whole genome shotgun sequence.
ACCESSION   DAOQOC010000362 DAOQOC010000000
VERSION     DAOQOC010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23810_length_2532_cov_3.968107"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..758
                     /gene="pth"
                     /locus_tag="PL106_06750"
     CDS             195..758
                     /gene="pth"
                     /locus_tag="PL106_06750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNA32804.1"
                     /translation="MKFLIIGLGNPGAEYANTRHNIGFAVLDALAEKQGCAFAPDRYA
                     DRAEFRHKGKTFICLKPQTFMNLSGKAVRYWMEQENIISERTLVITDDLALPFGKIRL
                     RASGSAGGHNGLTSIIVTIGTEVFPRLRFGIGSDFPRGRQADHVLSQWNKEEAAALGQ
                     RITVCCQAVLDFGLMGIGHAMNSFNTK"
     gene            814..1335
                     /locus_tag="PL106_06755"
     CDS             814..1335
                     /locus_tag="PL106_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNA32805.1"
                     /translation="MLSKKVEAALNAQIEIEASSSQAYLAMASWAETNGLSGTSAFLY
                     RHSDEERVHMLKLIKFVNDRGGRAHVPALKQPQKDYKSITDIFQTLLEHETGVTASIN
                     NVVDLCLKEKDYTTHNFMQWYVSEQIEEETLARTLIDKLELIGSDKAGLYLFDRDLQN
                     LTGTSGGAHDGNA"
     gene            complement(1339..>2532)
                     /locus_tag="PL106_06760"
     CDS             complement(1339..>2532)
                     /locus_tag="PL106_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HNA32806.1"
                     /translation="QATAWQRYSAGISFAGPDGVPVPCPTVALAMTPDSADIRALVVD
                     VGNGLATNFDSLDRAVHGAIALVNLHLSNAPPGAVNLHRSEKVALAIQHGAIGVVFAN
                     AVEGGVLLTGTASVTNQPIAIPAVCIGKEDAVRLRERLVIEKPVHAHITMRNSSIPIT
                     ARNVLATLHGTDKAEDVIVVGGHLDSWDLATGATDNGIGSYSIIDLARCYVALGLRPR
                     RTIVFALFMGEEQGLLGSTALVNEWARSGQLDHVKCMINLDMSGDPSGFNAFGPEHLR
                     TLVSKAQQDMRALDTTYAARASDAPWLHSDHQPFMLHGVATLNPICDLGERVYRCYHS
                     DCDDLRLVDPAVMASNVQRVGEMLWLLANADDLPDHFKDEELRSRLVAGGLEEKLRLH
                     GEWRW"
BASE COUNT          526 a          762 c          779 g          465 t
ORIGIN      
        1 ttgaaggcga tgaagatcat gacaggtgtg gaggagtcga tcatgatcaa ctacctgaaa
       61 gcgacccgct tggaacgagg actcctgctc aacttcggaa caccgagcct ccagatcaag
      121 cgcatggtat ttaccgcata gtggatggct tgcgagcggc ggaaatcggc gaagtcgatg
      181 aataaacctc agtcatgaag ttcctcatca tcggtctggg caatccgggc gcggagtacg
      241 ccaacacccg ccacaacatc ggattcgccg tgctggacgc cctggcggag aaacagggtt
      301 gcgcgttcgc ccctgaccgg tacgcggacc gtgccgaatt ccgccacaag ggaaagacct
      361 tcatctgctt gaaaccacag accttcatga acctcagtgg caaggccgtg cggtactgga
      421 tggagcagga gaacatcatc agcgaacgga ccctggtgat cacggatgac ctggcgctgc
      481 ccttcggcaa gatccgcttg cgggccagcg ggagtgcggg cggtcataac gggctcacca
      541 gcatcatcgt caccatcggt accgaggtct ttccgcggtt gcgcttcggt atcggaagtg
      601 attttccgcg cggcaggcaa gccgaccatg tgctcagcca atggaacaag gaagaggccg
      661 ctgccctggg ccaacggatc accgtgtgtt gccaagccgt gctcgatttc gggctcatgg
      721 gcatcgggca cgccatgaac tcgttcaata cgaaatagcc gtaccggtcg cgtacgttgc
      781 gacgcatacc ttagcaccca gatccattac gtcatgcttt cgaagaaggt ggaagccgcg
      841 ctgaacgcgc agatcgagat cgaggccagc agcagtcagg cctatctggc catggcgagc
      901 tgggccgaga ccaacggcct cagcggaacg tccgcattcc tgtaccgcca cagcgatgag
      961 gagcgcgtac acatgctcaa gctcatcaag ttcgtgaacg atcgtggcgg tcgcgcacat
     1021 gtgcccgcgc tcaaacaacc gcagaaggat tacaagtcca tcacggacat tttccagacc
     1081 ctgctcgaac acgagacggg cgtaacggcc agcatcaaca acgtggtgga cctctgcctg
     1141 aaggaaaagg actacaccac gcacaacttc atgcaatggt acgtgagtga gcagatcgag
     1201 gaggagaccc tcgcgcgcac gctcatcgac aagttggagt tgatcggttc ggacaaggcc
     1261 ggactctacc tgttcgaccg cgacctccag aacctgacgg gtacttcggg cggcgcacac
     1321 gacggtaacg cctgagcgct accagcgcca ttccccgtgc agccgtagtt tttcttccag
     1381 tcctccggcg accagtcggg accgcaattc ctcgtccttg aagtgatcgg ggaggtcgtc
     1441 cgcgttggcg agcaaccaca gcatttcgcc gacacgttgc acgttgctcg ccatcaccgc
     1501 gggatccacc aggcgcaggt cgtcacaatc gctgtggtaa cagcggtaca cacgttcgcc
     1561 caggtcgcag atcgggttga gcgtggccac gccgtgcagc atgaagggtt ggtggtcact
     1621 atggagccaa ggggcatcac tggcgcgggc ggcataagtc gtgtccaggg cacgcatgtc
     1681 ctgttgcgcc ttggatacga gggtacgcag gtgctcaggc ccgaacgcat tgaaccccga
     1741 agggtcgccg ctcatgtcca ggttgatcat gcacttcacg tggtccagtt ggccgctccg
     1801 ggcccattcg ttcaccaggg cggtactgcc caacaagccc tgctcttcgc ccatgaaaag
     1861 agcgaaaaca atggtgcgcc ggggcctcag ccccaacgcg acgtagcagc gggccaggtc
     1921 gatgatggag taagagccga tgccgttgtc cgtggctccg gtagccaggt cccagctgtc
     1981 caggtggccg cccaccacga tcacatcttc ggccttgtcc gttccatgca gcgtggccag
     2041 cacattgcgc gccgtgatcg ggatgctgct attgcgcatc gtgatgtgcg catgcaccgg
     2101 tttttcaatg acgagtcgtt cgcgcagccg gaccgcgtcc tctttgccga tgcatacggc
     2161 aggtatggca atgggttggt tcgtgacgga tgcggtgccg gtgagcagca cgccgccttc
     2221 gaccgcgttg gcgaacacca ccccgatcgc accgtgttgg atggcgagtg cgaccttttc
     2281 gctgcgatgc aggttcaccg cgcccggcgg ggcattggag aggtggaggt tcaccagtgc
     2341 gatggcccca tgcacggccc ggtccaggct gtcgaaattg gtggcgagcc cattgcccac
     2401 atcaaccacc aaggctcgga tatccgcgga atcgggtgtc atggccaagg caaccgtggg
     2461 gcagggcact ggtacgccgt cggggccagc gaacgaaatg cctgccgaat agcgttgcca
     2521 ggcggttgct tg
//
LOCUS       DAOQOC010000363         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23813_length_2532_cov_3.453371, whole genome shotgun sequence.
ACCESSION   DAOQOC010000363 DAOQOC010000000
VERSION     DAOQOC010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23813_length_2532_cov_3.453371"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..524)
                     /locus_tag="PL106_06765"
     CDS             complement(30..524)
                     /locus_tag="PL106_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA32807.1"
                     /translation="MKHILASTLLFASCHLFAQSGKLPSVTVETMDGKKVNTATWSNN
                     GKPMIINFWATWCAPCKRELSTIAEKYADWQKQTGVKLIAVSIDDARSVNRVAPYVNG
                     QDWDYEVVLDPNGDLKRALNVNNVPHTFLVDGKGNIVYQHNNYEPGDENELYKKVKEA
                     AAAK"
     gene            complement(626..2041)
                     /locus_tag="PL106_06770"
     CDS             complement(626..2041)
                     /locus_tag="PL106_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32808.1"
                     /translation="MKTLLTGVALSTGLLVSAQNQAWTATDINGNTVDIAAIVNSGKT
                     VLVDISAHWCGPCWAWHNSHIMNKLYHEFGPEGTGDLEIIWVDGDPASSMALLQGGSG
                     SQGDWLADMPFTAIGPNGQGNTLANIYNINAYPTLFMHCPGSTSGVEIQRTATWEQFL
                     TSWKNMCPAAFDNAVVDAHLLNLGDKEVCPGEHPYTDVFNQGSAALTSASIELLQNGN
                     VIETKQWTGSIAPFQHSIVNFETTTITGDEDYTMNLIVPNDGHLAGNTDEGSFSLAPT
                     APVSLVSLQLKTDQYGSETTWKLFNSNGTVVAQDPPGNYGANTVYTYDWVLDPTECYT
                     FKIYDQYGDGICCSYGNGYYRLRNTWGTADNFISGGQFGGEEDKGFMTPAMVSVEDHT
                     SNGFRVWPNPTTGLLNMEFDANTLATVDVFNVLGERVMTNTFNASGIRVMDLSSLNNG
                     VYYMNVLANGHTLTERITLSK"
     gene            2269..>2532
                     /locus_tag="PL106_06775"
     CDS             2269..>2532
                     /locus_tag="PL106_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32809.1"
                     /translation="MRTTATLALVSSLFLATAAQAQLLNVYLPGEGPLVNGTTVVKYG
                     QPSDNIVSQQLPVVLNAANSTEVGLKRYELSATAGTDNYFCWYD"
BASE COUNT          446 a          729 c          796 g          561 t
ORIGIN      
        1 cagtcttggc tccctgaagg ggccgcggct cacttggcgg cggccgcttc cttcaccttc
       61 ttgtagagct cgttctcgtc gcccggttcg tagttgttgt gctggtacac gatgttgccc
      121 ttgccgtcca ccaggaaggt gtggggcacg ttgttcacgt tgagggcacg tttcaggtcg
      181 ccgttggggt ccagcaccac ctcgtagtcc cagtcctgcc cgttcacgta cggggccaca
      241 cggttcacgc tgcgcgcatc gtcgatgctc acggcgatca gtttcacccc ggtctgcttc
      301 tgccagtcgg catatttctc ggcaatggtg ctcaactcgc gcttgcacgg cgcgcaccac
      361 gtagcccaga agttgatgat catgggcttg ccgttgttgc tccaggtggc ggtgttcacc
      421 ttcttgccat ccatcgtttc aacggtaacg gacgggagtt tgccgctctg ggcgaagagg
      481 tggcaggagg cgaacagaag agtggaggcg aggatgtgtt tcatgattgt ttgcagggac
      541 ggtgtgggca ctgtgcgaag atcgggccag aaaaaaccgg tggcgcggga gcaccaccgg
      601 cttcttcaac ggatcccgag gtgtgttact tgctcagggt gatgcgctcg gtgagcgtgt
      661 gaccgttggc cagcacgttc atgtagtaca caccgttgtt caacgagctg aggtccatca
      721 cgcggatacc gctggcattg aacgtgttcg tcatcacgcg ctcgccgagc acgttgaaca
      781 catccaccgt ggccaaggtg ttcgcatcga attccatgtt cagcaggccc gtggtgggat
      841 tcggccaaac gcggaagccg ttgctggtgt gatcctccac actcaccatc gccggggtca
      901 tgaagccctt gtcttcttcg ccgccgaatt gcccgccgct gatgaagttg tcggccgtgc
      961 cccacgtgtt gcgcaggcgg tagtaaccat tgccgtagga gcagcagatc ccatcgccgt
     1021 actggtcgta gatcttgaac gtgtagcact ccgtgggatc aagcacccag tcgtacgtgt
     1081 acacggtgtt ggcgccgtag ttgccaggag gatcctgggc cacgaccgta ccgttgctgt
     1141 tgaacagctt ccacgtggtc tccgagccgt actggtcggt cttcagctgc agggaaacca
     1201 gcgaaacggg tgccgtgggg gccaaggaga agctgccttc gtcggtgttg ccggccaggt
     1261 ggccgtcgtt gggaacgatc aggttcatgg tgtagtcctc atcgcccgtg atggtcgtgg
     1321 tctcgaagtt cacgatgctg tgctggaaag gagcgatgga accggtccac tgcttggtct
     1381 cgatcacgtt gccgttctgg agcagctcga tgctggcgct ggtgagggcg gcggagccct
     1441 ggttgaacac gtcggtgtac ggatgttcgc cggggcacac ctccttatca ccaagattca
     1501 gcaagtgggc atcgaccaca gcattatcga atgctgcggg gcacatgttc ttccagctgg
     1561 tgaggaactg ctcccacgtg gccgtgcgct ggatctccac accagaggtg ctgccggggc
     1621 agtgcatgaa caaggtggga taggcgttga tgttgtagat gttggccagg gtgttgcctt
     1681 ggccgttcgg cccgatcgcc gtgaagggca tgtcggccaa ccagtcgccc tgagaaccgc
     1741 tgccaccctg gagcagggcc atggagctgg cggggtcgcc gtccacccag atgatctcca
     1801 ggtcgccggt gccttccgga ccgaactcat ggtacagctt gttcatgatg tgggagttgt
     1861 gccaggccca gcagggtccg caccaatgcg cgctgatatc caccagcacg gttttgccac
     1921 tgttcacgat ggccgcgatg tccaccgtat tgccattgat gtccgttgcg gtccacgctt
     1981 ggttctgagc gctgacgagc agccccgtgg agagagcgac gccagtgagt aaagtcttca
     2041 tcgtcatggg tgatcggttt ttggtcggtt cttcagttca ggagatcccc gtgcaggccg
     2101 tacgatcggg tgtcagggag tgcgaactta gtcatcctgt cgcatccgtt gggcatggat
     2161 ccgtctccgc ttggaaccct aaaagcccgc gcagggtcat gagccaggcc atggggggcc
     2221 tacctttgcc agccgcccgg aacctcgggc cgtacacttg ttcaacgcat gagaaccacc
     2281 gctacactcg ccctggtcag ttcccttttt ctggccaccg ccgcacaggc gcaactgctg
     2341 aacgtttatc tgcccggaga aggcccgctg gtgaatggca ccacggtggt caaatacggc
     2401 cagccttccg ataacatcgt atcgcagcaa ctgcctgtgg tactcaacgc cgccaacagc
     2461 accgaagtag ggctgaagcg gtatgagctg agtgccacgg ccggcacgga caactatttc
     2521 tgctggtatg at
//
LOCUS       DAOQOC010000364         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23872_length_2529_cov_3.210994, whole genome shotgun sequence.
ACCESSION   DAOQOC010000364 DAOQOC010000000
VERSION     DAOQOC010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23872_length_2529_cov_3.210994"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PL106_06780"
     CDS             <1..386
                     /locus_tag="PL106_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA32810.1"
                     /translation="VEVCEEAKNATLTGDPVHLTNAITNLVDNAVKYSPERPRVTIAS
                     MGDGRMLYIAVKDNGIGIRREDQRHIFERFFRVHTGNVHDVKGFGLGLHYVDTIVRAH
                     GGRISVDSEPGKGSVFTIGLPVRNG"
     gene            448..1146
                     /locus_tag="PL106_06785"
     CDS             448..1146
                     /locus_tag="PL106_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA32811.1"
                     /translation="MSDEKGTILLVEDDQNLGFVVQDALRRKGYTVHLCRDGKEGLKY
                     FNEHAYDICVLDVMLPHKDGFSLAEDIRLTDPTVPIVFLTAKSQTEDRIAGFKAGGDD
                     YLTKPFSHEELMLRIEAILRRTRGKGENPRAKDVFGIGSYVFDHRNMMLDHPDGARKL
                     TKKEADVLRLLCMHRDQVLPRELVLNMVWGDDTYFLGRSLDVFISRLRKYLRADPRIH
                     INNVHGVGFRFQVD"
     gene            complement(1204..2037)
                     /locus_tag="PL106_06790"
     CDS             complement(1204..2037)
                     /locus_tag="PL106_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA32812.1"
                     /translation="MPIRSTPTFLYNGADPITPSPGHGYYRMKTTAVIVDDEEHCRIA
                     LGGILERRHPEIEVLGLATNVPDGIKLVNEKKPQILFLDIEMGKQTGFDLLQALGPDQ
                     PHVIFTTAHEGYAVKAIRFSALDYLLKPIDPDELSIAIGKASEAMRTPQSPDQFMALL
                     KNLAQPNGPDKRIALPVADGLELVNVDEILYCESDSNYTVVHQKDKKRLVISRTLKEF
                     EDMLDPAQFIRVHHSHLINVKHVKKYIRGEGGEVIMADGTNVAVSRRKKQELMDSLAR
                     L"
     gene            complement(2086..>2529)
                     /locus_tag="PL106_06795"
     CDS             complement(2086..>2529)
                     /locus_tag="PL106_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32813.1"
                     /translation="EALDLYLNLEHARSRDASGAEKFDYSINVDPSIDQEETLVPPLV
                     VQPFVENAIWHGMAGKAEKGRITLSVTRVGDDLVMAIEDDGAGRHAKKMEPVPGAAVK
                     KTSLATTITKARLDLVSKQRGRPAGFRYIDLPQGTRVEVLLPLSA"
BASE COUNT          483 a          726 c          816 g          504 t
ORIGIN      
        1 aggtggaggt atgcgaggag gcgaagaacg cgaccctcac cggcgatccc gtacacctca
       61 ccaacgccat cacgaatctg gtggacaacg cggtgaaata ctccccggaa cgcccgaggg
      121 tgaccattgc cagcatgggt gatgggcgta tgttgtacat cgcggtgaag gacaatggca
      181 tcggcatccg tcgcgaggac cagcggcaca tcttcgagcg gttcttccgc gtgcataccg
      241 gcaacgtgca cgatgtgaag ggcttcggcc tggggctgca ctacgtggat accatcgtgc
      301 gtgcccatgg cggacgtatc agcgtggaca gcgagccggg aaaagggagc gttttcacca
      361 ttggattacc ggtcaggaac gggtaggagc aatggaggat gatcacccga agatacccac
      421 cactgtacat gataaaaacc gtgttcgatg agcgacgaga aaggaacgat attgctggtt
      481 gaggatgacc agaacctcgg gttcgtggtg caggacgcgt tgcggcggaa gggatacacc
      541 gtgcacctgt gccgcgatgg caaggagggt ctgaaatact tcaacgagca cgcgtacgac
      601 atctgtgtgc tggacgtgat gctgccgcac aaggacggct tcagtctggc ggaggacatc
      661 cgcctgaccg atccgacggt gcccatagtc ttcctcacgg ccaagagcca gaccgaggac
      721 cgcatcgcgg ggttcaaggc cggtggcgac gattacctca ccaagccgtt cagccacgag
      781 gagctgatgc tgcggatcga ggccatcctg cgccgcacgc gcggcaaggg cgagaaccca
      841 cgggccaagg acgtgttcgg gatcgggagc tacgtcttcg accaccgcaa catgatgttg
      901 gaccaccccg acggtgcgcg caagctcacc aagaaggagg ccgatgtgct gcgcctgctg
      961 tgcatgcacc gcgaccaggt gctgccacgc gagctggtgc tcaacatggt ctggggtgat
     1021 gatacctatt ttctggggcg cagcctcgac gtgttcatca gccgcctgcg caagtacctc
     1081 cgtgccgatc cacgcatcca catcaacaat gtgcatggtg tgggtttccg cttccaggtc
     1141 gactgagccg ggttgaacgc gaacggcccc ggtaccgggg ccgttcagca agaggcgcgt
     1201 tgttcagagc cgcgccaggc tgtccatgag ttcctgcttc ttgcgccggc tcacggccac
     1261 attggtgccg tcggccatga tcacctcacc gccttcaccg cggatgtact ttttcacgtg
     1321 cttcacgttg atcaggtgcg agtggtgcac acggatgaac tgggcggggt cgagcatatc
     1381 ctcgaactct ttcagggttc ggctgatcac caggcgtttc ttgtccttct gatgcaccac
     1441 ggtgtagttg ctatcgctct cgcagtagag gatctcgtcc acattcacca gttccaggcc
     1501 atcggccacg ggcagggcga tgcgtttgtc cggcccattg ggctgggcga ggtttttcag
     1561 cagggccatg aactggtccg ggctttgcgg ggtgcgcatg gcctcggacg cttttcctat
     1621 cgcgatactc agctcatcgg gatcgatggg cttgaggagg tagtccaacg cgctgaaccg
     1681 gatggccttc acggcgtagc cctcgtgggc ggtggtgaag atcacatgcg gttggtccgg
     1741 accgagcgcc tgaagaaggt cgaaaccggt ctgcttgccc atctctatat cgaggaagag
     1801 gatctggggc tttttctcgt tcaccaattt gataccgtcg ggaacgttgg tggccaggcc
     1861 gagcacttcg atctcgggat ggcgccgctc gagaatgccg cccaaggcga tgcggcagtg
     1921 ctcctcgtcg tctacaatga ctgcggtggt cttcatgcgg tagtagccgt ggccaggcga
     1981 aggcgtgatc gggtcggccc cattgtaaag gaatgtcggg gtcgaacgga tgggcaagat
     2041 acgagccttg gcgatcggac agcgatcccc tgcgtaaaag aggggtcagg cgctcaaagg
     2101 gagcaggacc tccacccgtg ttccttgtgg gaggtcgatg taccggaacc cggcgggtct
     2161 tcctcgctgc ttgctcacca ggtccagccg ggctttggtg atcgtggtgg ccagggaggt
     2221 ttttttcacg gcggccccgg ggaccggctc catttttttc gcgtgccggc ccgccccatc
     2281 atcctcaatg gccatcacaa gatcatcgcc aacgcgcgtc accgacaggg tgatgcgacc
     2341 tttttccgcc ttgccggcca tgccgtgcca gatggcgttc tccacgaagg gttgcaccac
     2401 caacgggggc acgagcgtct cctcctggtc gatggaaggg tcgacattga tggagtagtc
     2461 gaacttttcg gcaccgctgg catcgcggga acgggcgtgc tccaggttga gatacaggtc
     2521 gagcgcctc
//
LOCUS       DAOQOC010000365         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23900_length_2527_cov_3.707524, whole genome shotgun sequence.
ACCESSION   DAOQOC010000365 DAOQOC010000000
VERSION     DAOQOC010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23900_length_2527_cov_3.707524"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PL106_06800"
     CDS             complement(<1..699)
                     /locus_tag="PL106_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA32814.1"
                     /translation="MIDRIIRFSIQHKAIIGLFTLALVLFGAYSLTQLPIDAVPDITN
                     NQVQVITTTPTLAAQEVEQFVTAPIERAMASLPDLVEIRSISRFGLSLITIVFEEDVD
                     IYFARTLVDQRLDEAAELIPPGAGKPTMAPVSTGLGEVYQYVIHPKPGYEGKFSPSEL
                     RTIQEWIVVRQLLGIPGVAEVNSYGGEMKQYEVSVDAKRLQSMGVTISELFTALQNNN
                     ENSGGAYIEKRPNSY"
     gene            complement(870..1220)
                     /locus_tag="PL106_06805"
     CDS             complement(870..1220)
                     /locus_tag="PL106_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32815.1"
                     /translation="MRRAAFLLTLALYAFSALDLHEWAHVPQSIAHWLEHHSDLGHHD
                     EDGGQHHDEHGDHDPFGCDEHGACTAFSTTGIAMEPQPLVLTVAPIAHTIAVPYEEGA
                     LAAFSGSKWNPPKA"
     gene            1831..>2527
                     /locus_tag="PL106_06810"
     CDS             1831..>2527
                     /locus_tag="PL106_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009420593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNA32816.1"
                     /translation="MYRTHTCGELRLADAGKTVTLAGWVQRSRAMGGLTFVDLRDRYG
                     ITQLSFNAERDKALCDQANALGREYVVEATGTVKERESKNKNIPTGEVELIITALKVL
                     NSAKTPPFTIEEETDGGDDLRMKYRYLDLRRANVRKNFELRHRMAIETRNYLSAQHFL
                     EVETPVLIKSTPEGARDFVVPSRMNHGEFYALPQSPQTFKQLLMVAGFDRYFQIVKCF
                     RDEDLRADRQPEFT"
BASE COUNT          494 a          772 c          794 g          467 t
ORIGIN      
        1 gtaactgttg ggccgcttct cgatgtaggc gccgccgctg ttctcattgt tgttctgcag
       61 cgcggtgaac agctcgctga tggtgacgcc catgctttgc aggcgcttcg catccacgct
      121 cacctcgtac tgcttcatct cgccaccgta gctgttcacc tcggccacgc ccggtatgcc
      181 gagcagttgc cgcaccacga tccactcctg gatggtgcgc agttcgctgg gactgaactt
      241 cccctcgtag cccggcttgg gatggatcac gtactggtac acctcgccaa ggcctgtgct
      301 caccggcgcc atcgtgggtt tgcccgcacc gggcggtatc aattccgcgg cctcgtccag
      361 ccgctggtcc accagggtgc gcgcgaagta gatgtccaca tcctcctcga acacgatggt
      421 gatcaggctg aggccgaagc ggctgatgga gcggatctcc accaggtcgg gcaaactggc
      481 catggcgcgt tcgatgggtg cggtgacgaa ctgctccacc tcctgcgcgg ccagcgtggg
      541 cgtagtggtg atcacctgca cctggttgtt ggtgatgtcg ggcaccgcat cgatgggcag
      601 ctgcgtgagc gagtacgcgc cgaagagtac cagcgccaac gtgaagagcc cgatgatggc
      661 cttgtgctgt atggagaacc ggatgatcct gtcgatcatg actaaatggc gtgaggaagt
      721 gagaaagggg gaaagtgaga aagggagaaa gggagaaagg gaggaaggga gaaggggagt
      781 gagggaaaag aggtgcacgc acggtcagtc acgaacatcc gtgcgcagag cacgggtgcg
      841 ttcacaggtc cacctatcgg cggactacct caagccttgg gagggttcca cttggaaccg
      901 gagaaggcag cgagcgcgcc ttcctcgtag ggtacggcga tcgtgtgcgc gatcggcgca
      961 acggtcagca ccaagggctg gggttccatc gcgatgccgg tggtcgagaa cgcggtacag
     1021 gcaccgtgct catcgcaacc gaaggggtca tggtcgccgt gctcgtcgtg atgctgaccg
     1081 ccgtcctcat cgtggtggcc caggtcgctg tggtgttcca gccagtgcgc gatgctctgc
     1141 ggcacatgcg cccactcgtg caggtccaat gcgctgaagg cgtaaagggc gagggtgagg
     1201 aggaaggcgg cgcggcgcat accggggcaa atgtaggctt caacgggagg aatggagctg
     1261 gtgttgcggg gggccaattg ctcgatgatt cgggcttgcc cccttttacc attcgactcc
     1321 gctccgctcc tcgggggcta tgcaccctgc ggagtgccgc tacgctgatg ctcagggccc
     1381 tcggggtcgc gctcttcgtt attactcctc gcgcggatcc ttcgacatcg ctcaggaccg
     1441 cgctgcgggg tagccgctcc gatcgcttac gcgaagtgcg gaactacagc agttcatgaa
     1501 ctgaactcgg tggcaacagc agtccaacac cttccggctt gctcctgaac gatggcccct
     1561 tttcaatggt ccttcctgaa ggtgtccgtt ggatcagatg atcaatagca tgggcgtccg
     1621 atcaaaaatc gaataagatc ttggagcgag gcgcggctgg cacacctcac cagcaccgca
     1681 ttcgtggttg ccgatacttt cacggtgaca cctgtattcg gcttgcgacg agcgaacttc
     1741 ggaacggtga cccccggacc gcccccgaaa gttgtgaccg accatccccg ccttgccact
     1801 accggtattt tcgccgccct tagcactacc atgtaccgta cccatacctg cggcgaactc
     1861 cgccttgccg atgccggaaa gaccgtgacc ctcgccggat gggtccagcg cagccgcgcc
     1921 atgggcggcc tcaccttcgt ggacctgcgc gatcgctacg gcatcaccca gctcagcttc
     1981 aatgccgagc gcgacaaggc cctctgcgat caggccaatg ccttgggccg tgaatacgtg
     2041 gtggaggcca caggaacggt gaaggagcgt gaaagcaaga acaagaacat ccccacgggc
     2101 gaggtggagc tgatcatcac ggcactgaag gtgctgaata gcgcgaagac cccgcccttc
     2161 accatcgagg aggagaccga cggcggcgac gacctgcgca tgaagtaccg ctacctggac
     2221 ctgcgccggg ccaacgtgcg caagaacttc gagttgcgcc accgcatggc catcgaaacg
     2281 cgcaactacc tgagcgcaca acactttctg gaggtggaaa cacccgtgct catcaagagc
     2341 acgcccgagg gcgcccgcga cttcgtggtg cccagccgca tgaaccatgg tgaattctac
     2401 gcgctgcccc aaagtccgca gaccttcaag caactgttga tggtggccgg tttcgaccgc
     2461 tacttccaga tcgtgaaatg cttccgcgac gaggacctcc gcgccgaccg ccagccggag
     2521 ttcaccc
//
LOCUS       DAOQOC010000366         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23935_length_2525_cov_4.073279, whole genome shotgun sequence.
ACCESSION   DAOQOC010000366 DAOQOC010000000
VERSION     DAOQOC010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23935_length_2525_cov_4.073279"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PL106_06815"
     CDS             <1..340
                     /locus_tag="PL106_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNA32817.1"
                     /translation="VDNGIRNAIIGNLDPFPLRTDQGALWENFLISERCKTLAYRGWW
                     GRSYFWRTVQQQEIDLVEEEGSDLRAYEFKLNRPEKGRIPLTFIRAYPHARTEVVHRG
                     NFRQFLDGPA"
     gene            complement(369..851)
                     /locus_tag="PL106_06820"
     CDS             complement(369..851)
                     /locus_tag="PL106_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32818.1"
                     /translation="MSPSAQAQQTMIMWAYGPFGTPGDAAYIVENDRIYQACGAYGSR
                     GPCIYVIEQDRVYHSDAFGRPGICAFTLDGNNLVRAQGSSCMRGSCALLLEGNKVFRG
                     EGPFCTKQEGAFFIEPGGGPSPTLVHMAEGPFATKSDALLQVKGPIDTIALLTVLAGF
                     "
     gene            complement(992..1906)
                     /locus_tag="PL106_06825"
     CDS             complement(992..1906)
                     /locus_tag="PL106_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32819.1"
                     /translation="MMRALLLHIACLPCALMAQELIVPPGTGDPMELHFNEVFMARNG
                     IMAIEGVRQLKRDGEPIRGKNEREEFRFDGSGRTVWSGIFFDRNNAATDTSCVTRIYD
                     ARGLLCEQRRNDRSGEFAQRDSLDGEGRCLRRTHVRIQGPVTGGLALMRPLETLISDE
                     RFSHTVITVPPDSGSAWRVTWHNEHGLPYREQTFRKDRWGYLRAIEDRNIITGRQGEV
                     TFRYDEKGRLAERIEQADRSTPAFVKHIWRYDNAGNVTLCDSWRNEKQTRHTEYLYED
                     GTLLLKAILAKDMETGLIHITRYTTRRR"
     gene            complement(1903..>2525)
                     /locus_tag="PL106_06830"
     CDS             complement(1903..>2525)
                     /locus_tag="PL106_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA32820.1"
                     /translation="ATINGYLDDHAFTMEALLALYGVTFDEGWVTEARKLADHAVLHY
                     LDESTGMFWYTSDADPPLIARKMEVSDNVIPASNSAICKALFILGHLLEDQRYMDIST
                     RQLNNMVDGMERYPSAHSNWAQALLMRTHPWYEVAITGPMALERREEFGQHYVPNAMF
                     LGTTGTSALPLLAEKTLGAATTLFVCEDKVCRLPVDNVAAAMKQMK"
BASE COUNT          439 a          798 c          787 g          501 t
ORIGIN      
        1 cgtggacaac gggatacgca acgcgatcat cggcaatctg gacccattcc cgctgcgcac
       61 cgaccagggc gccctgtggg agaatttcct gatatcggag cgctgcaaga cgctagccta
      121 tcgtggctgg tgggggcgtt catacttctg gcgcaccgtg cagcaacagg agatcgacct
      181 ggtggaggag gagggcagcg acctgcgcgc ctacgaattc aaactgaacc ggccggaaaa
      241 ggggcgcatc cccctcacct tcatccgggc atatccacat gcgcggacag aagtggtcca
      301 tcgggggaat ttccgccagt tccttgatgg tccggcgtag caaaggtctg tgtggcgggg
      361 ggctctcctc agaaccccgc cagtactgtc agcagggcaa tggtgtcgat gggtcccttc
      421 acctgcagca gcgcgtcgct ctttgtggcg aacggccctt cggccatgtg aaccagcgtc
      481 ggcgatggcc cgccgcccgg ttcgatgaag aaggctcctt cctgtttggt acagaagggg
      541 ccttcgccac ggaacacctt gttgccctcg agcaggagcg cgcacgagcc gcgcatacag
      601 gagctcccct gtgcccgcac gaggttgttg ccgtcgagcg tgaacgcgca gatgcctggc
      661 cgcccgaagg catcgctgtg gtacacccgg tcctgctcga tcacatagat gcaggggccg
      721 cgtgagccgt aagcgccaca ggcctgatag atgcggtcgt tttccacgat gtacgcggca
      781 tcgccggggg tgccgaacgg gccataggcc cacatgatca tggtctgttg cgcttgagcg
      841 ctcggggaca tcagcagggg cagcaggcag caggcagcgg gcagcaggca gtggaccctt
      901 cggataaccg ccgctttccg ctgcctgctg cccgctgcgg gctgcccgct ccggtacctc
      961 gtccagcacc tcgtcaagta cctcgtcaag ctcatcgtct tcgcgtggta tacctcgtta
     1021 tgtgtatcaa tcccgtctcc atgtccttgg cgaggatcgc cttcagcagc agtgtcccgt
     1081 cctcgtacag gtactcggtg tgacgcgtct gcttctcgtt gcgccacgaa tcgcaaagtg
     1141 tgacgttgcc tgcgttgtcg tagcgccaga tgtgcttcac gaacgcaggg gtactccgat
     1201 cggcctgttc gatccgttcc gcaagacggc ctttttcgtc gtaccggaac gtgacctctc
     1261 cctgtctacc ggtaatgatg ttgcggtcct cgatggcacg caggtaaccc caccggtcct
     1321 tgcggaatgt ttgttcacgg tacggcagac cgtgctcgtt atgccaggtg acgcgccatg
     1381 ccgatccgct gtccggagga acggtgatga ccgtgtggct gaaccgctca tcgctgatca
     1441 gggtctccag cgggcgcatg agggcgagcc ctccggtgac cgggccctgg atgcgcacgt
     1501 gcgtgcggcg caggcatcgg ccctccccat cgagcgagtc gcgctgggcg aactccccgc
     1561 tgcgatcgtt ccgccgctgt tcacacagca gcccccgagc atcgtagatc cgcgtgacac
     1621 aggaggtgtc cgtggcggcg ttgttccgat caaaaaaaat accgctccaa accgttcgcc
     1681 cggacccgtc gaaacggaac tcctcccgtt cattcttccc gcggatgggc tcgccatcgc
     1741 gtttcaattg ccgcacaccc tcgatggcca tgatgccgtt ccgggccatg aacacctcgt
     1801 tgaaatggag ttccatcggg tccccggtcc ccggtggcac gataagctcc tgggccatca
     1861 aggcacaagg gagacaggcg atgtgcagga ggagtgcgcg catcatttca tctgcttcat
     1921 ggcagcggcc acgttgtcca ccggcaaccg gcacaccttg tcctcacaaa cgaacagggt
     1981 ggtcgccgcc ccgagggtct tctcggcgag aagcggtaaa gcactggtgc ccgtggtgcc
     2041 caggaacatc gcgttgggca cgtagtgctg gccgaactcc tcccgtcgct ccagcgccat
     2101 ggggccggtg atggccactt cgtaccacgg atgggtgcgc atgagcaggg cctgggccca
     2161 gttgctgtgg gccgatgggt atcgttccat gccgtcgacc atgttgttca actggcgcgt
     2221 gctgatgtcc atgtaccgct gatcctccag cagatgcccc aggatgaaca acgccttgca
     2281 gatcgcgctg ttgctcgcgg ggatcacgtt gtcgctcacc tccatcttgc gggcgatgag
     2341 cggcgggtcg gcatcgctgg tgtaccagaa catgccggta ctttcatcaa ggtagtgtag
     2401 aacggcgtga tcggccagct tccgcgcttc ggtgacccag ccctcgtcga aggtgacccc
     2461 gtacagtgcg agcagcgcct ccatggtgaa ggcgtgatcg tcgaggtagc cattgatcgt
     2521 tgccc
//
LOCUS       DAOQOC010000367         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23971_length_2523_cov_4.638979, whole genome shotgun sequence.
ACCESSION   DAOQOC010000367 DAOQOC010000000
VERSION     DAOQOC010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23971_length_2523_cov_4.638979"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2440
                     /locus_tag="PL106_06835"
     CDS             <1..2440
                     /locus_tag="PL106_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32821.1"
                     /translation="SDVGAESITVTDGITTCPQICDGTMSVAFDCQVPMCSIAWYDVL
                     ANDLQGSGNTMDSMCAGQYFVQVTNGLGCIAIDTVHVIAPQPVTAVLAITQPTCADAC
                     DGEASVVTSGGAGPYTFDWSPDTLAGDGTDTITGLCAGNYMLTVMDSLGCAITLDVPV
                     PGPPPIQVQALLTHVSCNGACDGGIVLYATGGTGPLEFQWTPDPGPPQGTDSLYALCP
                     GDWSVTITDSTGCSITAVYTIIEPLELTTAIATVDNLCQGDCAGSVTATIGGGTVPYT
                     IVWLDAGGAVIAVGDSALNGLCAGDYGLQVTDSAGCMVTNAFSIQENPAITAALTVIG
                     ETCHGPCDGTASIITAGGTGDHVVSWADGNGNVFATDTTAVGGLCAGNWSVTITDSLG
                     CDSVFAFTILPWSPILPNEAVQQVSCNGACDGAVGIAATGGAGTLVFTWDPAATTDST
                     GSASGLCPGAWNLTITGAAGCDTTITVNITEPPAITITVDDVTDASCNSAFDGAVSVT
                     LAGGTGNLSASWSGPGGFVSGSDDIGSLQAGSYLLTVTDGNNCTVQQAVSVAALTTVV
                     AVAGADVTLCSGGTTILDGSSSQGAVQYTWTDGQGATMGTQAQLDPGSPPPGVWTFIL
                     TVTNGPCSDADTVSVTILSSPFADAGVDQYIFIDGVVGVGGQPAGPQGSAFLWSPDSL
                     LTDATVSNPEVSPPSTTWYVLMVTSPEGCVNTDSVLVTVVPEITIPTGFTPNGDGYND
                     TWVIDYMDRFPDCTVEIYNRWGEQLFRSVGYNTPWDGRYAGGLVPVGTYYYAIELNDE
                     RFPEPYTGPLTVIR"
BASE COUNT          469 a          741 c          833 g          480 t
ORIGIN      
        1 gagcgatgtg ggcgcggagt ccatcaccgt taccgatggg atcaccacat gtccgcagat
       61 ctgtgatggc accatgtcgg tggctttcga ctgccaggtg cccatgtgtt ccatcgcgtg
      121 gtatgatgtg ctggcgaacg acctgcaagg gtccggcaat acgatggaca gcatgtgtgc
      181 cggacagtat ttcgttcagg tgacgaacgg cctgggctgc atcgctatcg atacggtcca
      241 cgtgatcgca ccgcaaccgg tgaccgcggt actcgcgatc acgcaaccca catgtgccga
      301 tgcatgcgat ggggaggcct ccgtggtgac cagtggtggc gcagggccgt acactttcga
      361 ttggtcgccg gacaccttgg cgggcgacgg tacggacacc atcaccggat tgtgtgcggg
      421 caactacatg ctcacggtga tggactcgtt gggctgtgcg atcacactcg acgtgccggt
      481 tccgggtccg ccgccgatcc aggtacaagc cttgttgaca catgtatcgt gcaatggcgc
      541 ctgcgatggc ggtatcgtac tgtatgctac tggaggaacg ggaccgctgg agttccaatg
      601 gacgccggac ccgggtcccc cgcaagggac ggacagcctg tacgcgctct gtccgggtga
      661 ttggagcgtg accatcacgg acagcaccgg ttgctccatc acggcggtgt acacgatcat
      721 cgagccgttg gagttgacca cggccatcgc cacggtggac aacctgtgcc aaggggattg
      781 cgcgggaagc gtcacggcaa cgatcggtgg gggtaccgtc ccgtacacca tcgtatggct
      841 ggacgctggc ggcgcggtga tcgctgtggg cgacagtgcg ttgaatggac tttgcgctgg
      901 cgattacgga ttgcaggtga ccgacagcgc cggttgcatg gtgacgaacg cgttcagtat
      961 ccaggagaat ccggcgatca ccgcggcact gaccgtgatc ggcgagacct gtcatggtcc
     1021 gtgtgacggt accgcatcga tcatcacagc aggcggcacc ggcgatcatg tggtctcctg
     1081 ggccgatggg aacggcaacg tgttcgccac ggacaccaca gcggtgggcg ggctctgtgc
     1141 gggcaactgg tcggtgacga tcacggacag cctcggatgc gacagtgtgt tcgcgttcac
     1201 gatcctgccc tggtcaccga tccttcccaa tgaagcggtt caacaggtgt cgtgcaatgg
     1261 ggcgtgcgat ggggcggtag gcatcgccgc gacaggcggt gcgggaacac tcgtgttcac
     1321 ctgggaccca gcggcaacga ccgactccac gggttccgca tcgggcttgt gccctggggc
     1381 atggaacctg accattaccg gcgcagctgg ttgtgatacc acgatcacgg tcaatatcac
     1441 cgagcctccg gcgatcacga tcacagtgga cgatgtgacc gacgcaagct gcaattcggc
     1501 ctttgatggg gcggtgagcg taacgttggc cggtggcacg gggaacctct cggccagttg
     1561 gagcggaccc ggtggttttg tatccggttc cgatgatatc ggctcgttgc aagcgggaag
     1621 ctacctgctt acggttacgg acgggaacaa ctgcactgtg caacaagcgg tgtccgtggc
     1681 ggcattgacc accgtggtgg ccgtcgcggg cgctgatgtg acgctatgtt ccggtggaac
     1741 gaccatcctg gacggatcct ccagccaggg agcggtgcag tacacctgga ccgatggcca
     1801 gggtgcgacc atgggtacgc aggcgcagct tgatccgggg agcccgccac cgggcgtatg
     1861 gaccttcata ctgacggtga ccaacgggcc ctgttcggac gcggataccg tatcggtgac
     1921 gatcctgtcc tctcccttcg ccgatgccgg ggtggaccag tacattttca tcgatggggt
     1981 cgtgggggtc ggtggtcagc cggcaggccc gcaagggagc gccttcctgt ggtccccgga
     2041 ttcattgctt actgatgcta cggtatcgaa cccggaggtt tcgccaccct cgaccacgtg
     2101 gtacgtcctg atggtgacct ctcccgaggg ctgtgtgaac accgacagcg tgctggtgac
     2161 cgtcgtgccg gagatcacga tcccgacggg cttcacaccg aacggcgacg ggtacaacga
     2221 tacctgggtg atcgactaca tggacaggtt cccggattgc acggtggaga tctacaaccg
     2281 ctggggcgag caactcttcc ggagcgtggg ttacaacacg ccatgggacg ggcggtacgc
     2341 gggcggcctt gtacctgttg gcacgtatta ctacgccatt gaactgaacg acgaacgctt
     2401 ccccgagccc tataccgggc cgctcaccgt gatccgctga ccccatgcac acgcaacgca
     2461 cccactgggc caaggcacac aaaacacgct cctgcgttca acacatgggc acgagacgca
     2521 tgc
//
LOCUS       DAOQOC010000368         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_23972_length_2523_cov_3.642220, whole genome shotgun sequence.
ACCESSION   DAOQOC010000368 DAOQOC010000000
VERSION     DAOQOC010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23972_length_2523_cov_3.642220"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2338)
                     /locus_tag="PL106_06840"
     CDS             complement(<1..2338)
                     /locus_tag="PL106_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32822.1"
                     /translation="MKQLERILIGILLVSSMASQAQKSPLNLPEVWMNVRYNTAEHMF
                     MDEDRAPQPDYTLIKATKPGKGGGLWRVFTDRENVALFDAPEGKETRRLPKLSTRLYV
                     YKEKGPWVLVSEESTGPTQHWCHKRDLVLWNTPLGDHATGIELKAFVVNTTKAGRALV
                     QDPSKKERYEVLAAPSAKATKIKEQFIYDVLFVYKEYVDPSSGDAYFLVSDEIQISGG
                     EGLGLLGWIHGSRVKTWETRLCLEPNFDPAAIAERREKNIRAKLFLQGDKAGLERYLQ
                     DGTGRGVADNTARDPAMGLDPERYPRIDGKLFRYPVLLASNTGMGPDNCRFLTGVSGY
                     FTPSSSGQLEEYAPERYHELDQVRQRRKERMNDVNLVFVLDGGLGTEQFRAAATTVVD
                     KLARAGSTMSIEYSAVIYRNEFADMNEAADPENNYCETIGPLSNAGQFGARLASVVPR
                     NSGDPSGQRAVHFALKRAIGFCQDNETNIIVHIGARPDVLAGNPFFVSAKANGGTRVE
                     PTDVSDLLSINKPVHYLGYVGWDDKVPQRERERLYEGLEEMMQNMANRIKNSFGGGMS
                     MAETGRDVESARAVETRQGGLSVIRMDHMGSWLMKTTMLPDKNSNNTLAASIRLNIDS
                     CLLLAQKLVTELDQLFQNDSPLSERAGDLTNSAAMDVLLDGIDPSEHDAVRAILSKEK
                     VHMFVDSRTVYKAGGLTHPIFRYVLFYEESKLNDQLRDLTDLNQKLDAGDPAMVAMAF
                     REYWMKKAEGVLGSGYTKATIKVEDLLERLHGIQDLSGM"
     gene            complement(2343..>2523)
                     /locus_tag="PL106_06845"
     CDS             complement(2343..>2523)
                     /locus_tag="PL106_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32823.1"
                     /translation="EPRYLVGVRAQWDGMARNSGVVLDHRWFTHPDDKERPRLGSLTS
                     SRYRLFVGITMGIRL"
BASE COUNT          413 a          804 c          746 g          560 t
ORIGIN      
        1 ccatgccgct caggtcctgg ataccgtgca ggcgctccag caggtcctcc acttttatcg
       61 ttgccttggt gtaaccgctt cccagcacac cttcggcttt cttcatccag tattcacgga
      121 aggccatggc caccatggcc gggtcgccgg cgtcgagttt ctggttcagg tccgtgaggt
      181 ccctgagttg gtcgttcagt ttgctctcct cgtagaacag cacgtatcgg aagatcgggt
      241 gcgtgagccc ccctgccttg tacaccgtgc ggctatccac gaacatatgc accttttcct
      301 tgctcaggat ggcacggacg gcatcgtgct cgctcggatc gatgccatcg agcagcacgt
      361 ccatcgcagc gctgttcgtc aggtccccgg cgcgctccga gagcggcgag tcgttctgga
      421 acaattgatc caactcggtg accagttttt gggccagcag gaggcacgaa tcaatgttga
      481 gcctgatcga tgcggccagc gtgttgttgc tgttcttgtc cggcagcatc gtggtcttca
      541 tcagccagct ccccatgtgg tccatacgga tcacggaaag gcccccttga cgcgtctcca
      601 ccgccctcgc cgattccaca tcgcggccgg tctcggccat gctcatgccg ccgccgaacg
      661 agtttttgat gcgattggcc atgttctgca tcatttcttc cagtccctca tacaggcgct
      721 cgcgttcgcg ttgcgggacc ttgtcgtccc aacccacata cccgaggtag tgtacgggct
      781 tgttgatgct cagcagatcg gacacatcgg tgggctccac ccgggtgccg ccattggcct
      841 tggccgatac gaagaacgga ttgcccgcca ggacatcggg ccgggccccg atatggacga
      901 tgatgttggt ctcattgtcc tggcagaacc cgatggcgcg tttcagggcg aaatgcacgg
      961 cccgctgacc gctggggtcg cccgaattgc ggggaaccac cgacgccaag cgggcaccga
     1021 actggcctgc gttgctcaac ggtccgatgg tctcgcagta gttgttctcc ggatcggccg
     1081 cctcgttcat gtcggcgaat tcgttgcgat agatcacggc gctgtattcg atggacatgg
     1141 tactgccggc ccgggcaagc ttgtccacca cggtggtggc cgccgcgcgg aactgctcgg
     1201 tgcccagccc gccgtccagc acgaacacca ggttcacatc gttcatgcgc tctttgcgcc
     1261 gttgccgcac ctggtccagt tcgtggtacc gttcaggggc gtattcctcc aactggcccg
     1321 aactggatgg tgtgaaatat ccggaaacac cggtaaggaa acggcagttg tccgggccca
     1381 tgcccgtgtt gctcgccagc aggaccgggt agcggaacag cttcccatcg atacgcggat
     1441 accgctccgg atccaatccc atggcgggat cgcgggccgt gttgtcggct acaccacgac
     1501 ccgtgccgtc ctgcaggtag cgttcaagcc cggccttgtc gccttgcagg aacagtttcg
     1561 cccggatgtt cttctccctt cgctcggcga tggccgccgg gtcgaagttg ggctccaggc
     1621 aaaggcgtgt ctcccaggtt ttcactcgcg aaccatggat ccatcccaac aggccgaggc
     1681 cttccccgcc gctgatctgg atctcgtcgc ttaccaggaa ataggcgtcg ccgctcgagg
     1741 ggtccacata ctccttgtac acgaagagca catcgtagat gaattgctcc ttgatcttcg
     1801 tggcctttgc cgagggtgcg gcgagcacct cgtaccgttc cttcttgctg gggtcctgaa
     1861 cgagggcacg acccgccttg gtggtgttca ccacgaacgc cttcaattcg atccccgtgg
     1921 cgtggtcgcc caagggagtg ttccacagca ccaggtcgcg tttatggcac cagtgctggg
     1981 tcggccccgt gctctcttcg ctcaccagta cccacgggcc tttctccttg tacacgtaca
     2041 ggcgggtgct caacttgggc agccgccggg tttccttgcc ttcgggtgcg tcgaacaacg
     2101 ccacgttctc ccgatcggtg aatacccgcc agagcccgcc ccctttgccc ggcttggtgg
     2161 ctttgatcag ggtgtagtcc ggttgcggtg cacggtcctc gtccatgaac atgtgctcgg
     2221 ccgtgttgta ccgaacgttc atccacactt ccggtaggtt gagcgggctc ttctgtgcct
     2281 ggctggccat tgagcttacc agcaggatgc cgatcagaat tcgttcgagt tgcttcatcg
     2341 tcttagagcc ggatacccat ggttatgcca acgaacaggc ggtagcggga ggaggtgagc
     2401 gagcccagtc tgggccgttc cttgtcgtcg ggatgtgtga accaccggtg gtccagaacg
     2461 acgccggaat tccgggccat gccatcccac tgggcccgca cgccgaccag gtagcgcggt
     2521 tcg
//
LOCUS       DAOQOC010000369         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24464_length_2497_cov_3.702293, whole genome shotgun sequence.
ACCESSION   DAOQOC010000369 DAOQOC010000000
VERSION     DAOQOC010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24464_length_2497_cov_3.702293"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1221
                     /locus_tag="PL106_06850"
     CDS             76..1221
                     /locus_tag="PL106_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNA32824.1"
                     /translation="MRKVYLDNAATTPLDPEVLDAMLPYMREHHGNPSAIHSFGRTTR
                     AAVEQARRKIASLLNCSPGEIIFTSGGTEADNMAIFGSVRDLGVENLITSPIEHHAVE
                     YVAHALHAEGRARIHWADLDAQGRVDMGHLEQLLKANPKALVSLMHANNEIGTRIDLH
                     AVGALCRKYGALFHSDTVQTMGHYRFDLAQLPVDFITCAAHKFHGPKGIGFLFMRNGA
                     TLKPMILGGSQERNMRAGTENLYGIVGLAKAMELAYRDLDEHIRHVTGLKKRMMARLI
                     ELIPGIVINGDPGDDALYTVLSVHLPEDGRSEMLIYNLDIEGIACSGGSACSSGSNKG
                     SHVIGYLYPGREGANVRFSFSKFTTLEEVDRAAEVLAKVYAPVAVKG"
     gene            complement(1265..>2497)
                     /locus_tag="PL106_06855"
     CDS             complement(1265..>2497)
                     /locus_tag="PL106_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32825.1"
                     /translation="NYQFDIDGQNRFFYTRRIGIENGMPVPVLGGARLFGKQGRHNIG
                     ALVLQTGATGDAPTTNNAVFRYKHDIGEQSFVGGLVTSKINADVSSQVVAVDGGWNSN
                     KFLGDKRIALEGWYARSITDGELDSNGTSAGLWFAYPNDRWDCAALIGTTEGGFKPEL
                     GFLNRTNYELAEAEVNFQPRWFQKLGVRKMLFNLAEVSMYRTAHTGNVESFSFSTSPL
                     GVLLGKGDEAGLWFAWFEDGVAEAFDISDDISIAAGRYSYRETGFYVSTFRGRRLQME
                     WNGSWGTFFGGKKTTYNTSITGNFNKHFNVQLDHTWNALEFPVNDLRNKVRLETHEVA
                     LYPTWAFNPNLSVSIFGQWNSLQDIGLVNARLHWIPKIGTDLFLVFNQSHSPTDRIRI
                     DDPASRRVVGKLVWRVMF"
BASE COUNT          471 a          763 c          792 g          471 t
ORIGIN      
        1 tggccaagcg gatcatgggc gagatggcag ggctcactga acggatctga taccgggcat
       61 cccacctttg tccccatgcg caaggtttac ctcgacaacg ccgccaccac accgctcgac
      121 cccgaagtgc tggatgccat gctgccgtac atgcgcgagc atcacggcaa ccccagtgcc
      181 atccacagtt tcggccgcac cacccgtgcc gccgttgaac aggcccggcg aaagatcgct
      241 tcgttgttga actgctcgcc cggcgagatc attttcacga gcggcgggac cgaagcggac
      301 aacatggcca tattcggaag cgtgcgcgac ctcggggtgg agaacctgat cacctccccg
      361 atcgagcacc acgcggtaga atacgtggcc catgccctgc atgccgaggg cagggcgcgg
      421 atccactggg cggacttgga tgcccaggga cgcgtggaca tgggccattt ggaacagctg
      481 ctcaaggcca accccaaagc cctggtgagc ctgatgcacg ccaacaacga gatcggcacg
      541 cgcatcgacc tgcatgcggt cggggcgttg tgccggaagt acggtgcgct tttccacagc
      601 gatacggtcc agaccatggg gcactaccgc ttcgacctgg cgcaactgcc cgtggacttc
      661 atcacctgtg cggcgcacaa attccatgga cccaaaggca tcggcttcct gttcatgcgg
      721 aacggggcca cgctgaagcc catgatcctc ggcggctcgc aggagcgcaa catgcgcgcc
      781 ggtacggaga acctgtacgg gatcgttggc cttgccaagg ccatggagtt ggcctaccgg
      841 gacctggatg agcacatacg ccatgtaact gggctcaaga agcgcatgat ggcgcgcctg
      901 atcgaattga tccccggcat cgtgatcaat ggcgaccccg gggacgatgc cctgtacacg
      961 gtgctcagcg ttcaccttcc agaggatggc cggagtgaga tgctgatcta caacctggac
     1021 atcgagggca tcgcgtgcag cggcggcagc gcgtgcagca gcggcagcaa caagggctcg
     1081 cacgtgatcg ggtacctgta cccggggcgc gaaggcgcga atgtccgttt ctcattcagc
     1141 aagttcacca cgctggagga ggtggaccgg gccgcggagg tgctggccaa ggtgtacgca
     1201 ccggtggccg tgaagggctg atccggttcc cccatcggac gcaaggacaa cgcctccatc
     1261 gggatcagaa catcacccgc cagaccagtt tgcccaccac tctgcgcgag gccggatcgt
     1321 cgatgcggat gcggtcggtg ggggagtgcg actggttgaa aaccaggaag aggtctgtgc
     1381 cgatcttcgg gatccagtgc aggcgggcat tcaccaggcc gatgtcctgc aagctgttcc
     1441 actgaccgaa gatggagacg gacaggttcg ggttgaatgc ccaggtggga tagagggcca
     1501 cttcgtgcgt ttccagccgg accttgttac gcaggtcgtt gacggggaac tccaaagcgt
     1561 tccaggtgtg gtccagttgc acgttgaaat gcttattgaa gttgcccgtg atgctcgtgt
     1621 tgtacgtggt cttcttcccg ccgaaaaagg tgccccagga tccgttccat tccatttgca
     1681 ggcgccggcc gcggaaggtg gacacgtaga acccggtctc ccggtaacta tagcgtccgg
     1741 ccgcgatgct gatgtcgtcg ctgatgtcga aggcctcggc caccccgtcc tcgaaccagg
     1801 cgaaccagag ccctgcctcg tcgcctttgc ccaggagcac gccgagcggc gatgtgctga
     1861 atgagaacga ttccacgttg ccggtatgtg cggtgcggta catgctcact tcggccaggt
     1921 tgaagagcat cttgcgcacg cccagcttct ggaaccagcg cggttggaag ttcacctcgg
     1981 cttcggccag ctcataattg gtgcggttca ggaaaccgag ttcgggtttg aagccgccct
     2041 cggtggtgcc gatcagggcc gcgcagtccc agcggtcatt cggataggcg aaccagagtc
     2101 ccgcgctggt gccgttgctg tcgagttcgc catcggtgat gctgcgggcg taccaacctt
     2161 ccagggcgat gcgtttgtca ccgaggaatt tgttgctgtt ccagccgccg tccacggcca
     2221 ccacttggct gctcacgtcc gcattgatct tgctggtgac cagtccgccc acgaagctct
     2281 gctcgccgat atcgtgcttg tagcggaaca cggcattgtt ggtggtgggc gcgtccccgg
     2341 tggcgccggt ctgaagcacc agcgccccga tgttgtgtct gccctgtttg ccgaagagcc
     2401 gcgcaccgcc cagcacgggc accggcattc cgttctcgat gccgatgcgg cgggtgtaga
     2461 aaaagcggtt ctgcccgtcg atatcgaact ggtaatt
//
LOCUS       DAOQOC010000370         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24533_length_2493_cov_3.312141, whole genome shotgun sequence.
ACCESSION   DAOQOC010000370 DAOQOC010000000
VERSION     DAOQOC010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24533_length_2493_cov_3.312141"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PL106_06860"
     CDS             <1..227
                     /locus_tag="PL106_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA32826.1"
                     /translation="FTTVEDLDARIDAHAKELAAYSPEAMAELKNVMWSGTEHWDNLL
                     TERAAISGRLVLSDHTRNAIAKFKAATAEK"
     gene            complement(280..429)
                     /locus_tag="PL106_06865"
     CDS             complement(280..429)
                     /locus_tag="PL106_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32827.1"
                     /translation="MEHNMEWAGDPWSVTLGRGPAVQLKPNAGIGQGRKREVRVHVVD
                     RTVEV"
     gene            complement(434..2014)
                     /locus_tag="PL106_06870"
     CDS             complement(434..2014)
                     /locus_tag="PL106_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32828.1"
                     /translation="MPWPSKAAALAIVLPWSICTTATGQGFSRRYDPIGSTGQFGWCI
                     EQIGADQFVVIGNMPWTDSLYYPSTVISFKLDPNGELIEEHQFQIPLKASYPGWSNSG
                     VVLSDGRVVIGGGTQDLSDSNRVAIFWLGENSEPIDYLEIRPSAHHWIGYQLKATPDG
                     GFILTGVTTATGVQDIFLLKTDSIGNVQWWQTYGHPTRLDFCATVDLAPDGGYFIGGL
                     YQQIPGNGGQYVQWVLHADSLGGLLWEYFGGVPSYEAANAAVLATQDGNLVYAAALHG
                     VGQNADDRPQLVKLDTGGTELWNRTYGGEFYGTGFFVVAEVPISGDLIACGQKWFTGL
                     NVPPYRKGILLRATSEGDSLWMREYLYSDSTVVDCQGVFRDVQPTPDGGFIVVGEARG
                     NINGSNPPGLSVDVWVVKVDSLGCIIPGCDDLSTPITEQITNLKEAVTVFPNPTSTST
                     VVKVTLPSGSALLGRGVGGEGELTLRLVSTQGQEVLVQKAVMGENALDLRALQAGLYY
                     LHLTNGSTWLGGIKLVKQ"
     gene            complement(2203..>2493)
                     /locus_tag="PL106_06875"
     CDS             complement(2203..>2493)
                     /locus_tag="PL106_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32829.1"
                     /translation="NIPLGITTQITNLKGALCVAPNPAHGQCTVQVELPETLRSAHLR
                     FAVVSAQGRLIREQKVAHGSNTLDLSGLAPGLYHLHLANSDTWLAGAKLIIE"
BASE COUNT          536 a          792 c          667 g          498 t
ORIGIN      
        1 tattcaccac ggttgaagat ctggatgccc ggatcgatgc gcacgccaag gaactcgccg
       61 catacagccc cgaggccatg gccgaactga agaacgtgat gtggagcggt acggagcact
      121 gggacaactt gctcaccgaa cgtgccgcga tcagcgggcg gttggtgctc agcgatcaca
      181 cccggaacgc catcgcgaag ttcaaagcgg ccactgcgga gaagtagcca ggcgccgcga
      241 agtcgcaccg gaagaaagca tgagcggcga gtaggtgcgc tatacctcca ccgtacggtc
      301 caccacgtgt acgcgcactt ctcgtttgcg gccctgtccg atcccggcgt tcggcttcag
      361 ctggaccgct ggccctctgc cgagtgtcac actccacggg tctccggccc attccatatt
      421 gtgttccatg gccctattgt ttcaccaact tgatcccgcc caaccacgtg ctaccgttgg
      481 tgaggtgaag gtagtacaac ccggcttgca aggcccgcag atccaacgcg ttctccccca
      541 tcaccgcctt ttgcaccagc acttcctggc cttgggtgct gaccaaccgg agcgtgagtt
      601 cgccctcacc cccgacccct ctcccgagga gagcggagcc tgagggcagc gtgactttca
      661 ccacagtgct cgtgctcgtt gggttgggaa agaccgttac cgcctccttc aaattggtga
      721 tctgctcggt gatgggggtg gaaagatcat cgcatccggg gatgatgcag cccaggctgt
      781 cgactttcac cacccacaca tccacactga ggcccggcgg attgctgccg ttgatgttgc
      841 cgcgcgcctc tccaaccaca atgaatcccc catccggtgt gggttgcaca tcgcggaaca
      901 ccccttggca atccaccacc gtgctgtcag aatacaaata ctcgcgcatc cataggctat
      961 cgccctcgct ggtggcacgc aacaagatac ccttcctgta gggaggaaca ttcagcccag
     1021 tgaaccattt ctggccacag gcgatcagat cgcctgagat cggtacttca gcaacaacaa
     1081 aaaatccggt tccatagaac tccccgccat atgttctgtt ccacaactcc gtcccacccg
     1141 tgtcgagctt gaccaattgt ggccgatcat ccgcattctg acccacaccg tgcaaagcag
     1201 ccgcatagac gaggttccca tcctgagtgg cgaggaccgc agcattggcc gcctcatacg
     1261 atggcacgcc accgaaatac tcccacagca agccgccaag gctgtccgca tgaagcaccc
     1321 actgcacata ttgcccacca tttccaggta tttgttgata caaaccacca atgaagtaac
     1381 caccatccgg tgcaagatcg accgttgcgc aaaaatccaa ccgtgtcggg tgaccgtagg
     1441 tctgccacca ctgcacgttg ccgatgctgt cggtcttgag caggaagatg tcctgtaccc
     1501 ctgtggcagt ggtaacacct gtcagaatga aaccaccatc aggggtggcc ttcagttgat
     1561 agccgatcca atggtgtgcc gaaggccgta tctcgaggta gtcgatcggc tcggaattct
     1621 caccaagcca gaaaatggcc actctatttg aatcggagag atcctgtgtt ccacctccga
     1681 tcacgaccct accatcactc agcaccacac ctgaattcga ccagcccgga taggaggcct
     1741 tcagcggaat ttgaaactgg tgctcttcga tcaattctcc gttgggatca agcttgaaac
     1801 tgatcacggt agaagggtag tacaacgaat cggtccaagg catattgccg atcactacga
     1861 actggtcggc accaatctgc tcgatgcacc agccgaactg accagtgctg ccgatcggat
     1921 cataccgtcg gctaaagcct tggccggttg ccgtggtgca aatgctccac ggcaacacga
     1981 tggccagagc agcagccttt gaaggccaag gcatggctca gcgcaaagcg atgaacttca
     2041 ccacccccac caggatgcca tcggcgcgca aggccacagc gtaaacccca ggtggcaact
     2101 ggttctgtgg cagtaccaca ataccgccct tccggcgctc ggctgtgccg agtgcccatt
     2161 cgttcttggg cctccggccc ttcctgtgat cgttcgcacg gcctattcga tgatgagttt
     2221 tgcccccgcc aaccacgtat cgctgttggc caggtgcagg tggtacaaac ccggggcgag
     2281 gcccgagagg tccaaggtat tgctgccgtg cgccaccttc tgttcgcgga tcaatcggcc
     2341 ctgagcgctg accacggcga agcgaagatg cgcgctgcgc aaggtctccg gtaattctac
     2401 ctgcaccgtg cattggccgt gcgccgggtt gggggctacg cacagcgctc ccttcaaatt
     2461 ggtgatctgt gtggtgatgc ccagggggat gtt
//
LOCUS       DAOQOC010000371         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24601_length_2489_cov_3.875103, whole genome shotgun sequence.
ACCESSION   DAOQOC010000371 DAOQOC010000000
VERSION     DAOQOC010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24601_length_2489_cov_3.875103"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PL106_06880"
     CDS             complement(<1..183)
                     /locus_tag="PL106_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32830.1"
                     /translation="MHRIIHHVERGQGIPLVLLHGFPFDHTLWLPQLEELTDAARMIA
                     PDLRGFGGSHGVPEVMT"
     gene            complement(187..558)
                     /locus_tag="PL106_06885"
     CDS             complement(187..558)
                     /locus_tag="PL106_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32831.1"
                     /translation="MSFLHLVKYGTLTFFAAAMLACGSHRNAAESDAAIRLQEANQGF
                     STGRVTTEFIGDGCPLLIRVDHAKNLYLYPIGLEEKFRKHGLRLTFKYRPVKASTGDC
                     RKGSAAEVDEVSLTPTMPKDQ"
     gene            complement(863..1186)
                     /locus_tag="PL106_06890"
     CDS             complement(863..1186)
                     /locus_tag="PL106_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNA32832.1"
                     /translation="MKKRTLTSFGELAWSSGPQGSGKPTAAPGQQDLRIHLDRLGGNK
                     TVTRIVGFTGKDADLDDLGRALKSKCGVGGNTKDGVILLQGDHRDKVLAYLTEKGYKA
                     KKAGG"
     gene            1235..2365
                     /locus_tag="PL106_06895"
     CDS             1235..2365
                     /locus_tag="PL106_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32833.1"
                     /translation="MRGVVTVIPVRGAKELAHFIRLPFQLKDQRKNHVPPLLADEREF
                     HDPKKNPQLAHGEVERWIAWRGDQAVGRIMGIIHRSYNAKQSERTARFYQLDSERDGE
                     VVRALIGAVEEWARSKGMDRMIGPFGFSDKDPQGVQIAGFEQLPVIATPTNADWLPAM
                     VEACGYTQFEDMVSYRMDIPARLPERYHAIADRCLRNLGSHRVPLGSKRELKPWVVPV
                     LRLVNATYGDLLGFAPMSEEEMHVLAKKYMYILDPQLVHLIADAKNEPVAFVIASPDM
                     SEGLVRANGKLFPLGFVHILRAMKRSKQLDLLLGAVRPDLQGKGLTAALGISLFDAAR
                     QHGFTHLDSHLIMERNTRMRSELERLGAVVWKRYRVYQRGLS"
BASE COUNT          451 a          734 c          805 g          499 t
ORIGIN      
        1 cgtcatcacc tccggcaccc cgtgcgaccc accgaaaccg cgcaggtccg gtgcgatcat
       61 tcgtgcggca tcggtgagtt cctccaattg cggcagccag agcgtgtgat cgaacgggaa
      121 gccatggagc agcacgagcg ggatgccctg gcctcgttcc acatggtgga tgatccggtg
      181 catgacctac tgatccttcg gcatcgtcgg tgtcaggctc acctcgtcca cttcggccgc
      241 ggagcctttg cggcaatcgc ctgtactggc cttcaccgga cgatacttga aggtgaggcg
      301 aagaccgtgt ttcctgaatt tttcctccag gccgatgggg tagaggtaca ggttcttcgc
      361 gtggtccacg cggatgagca gaggacagcc atcgccgatg aactccgtgg tgacgcggcc
      421 ggtgctgaac ccctggttgg cttcctgtag ccgaatggcg gcatcgcttt ccgcggcatt
      481 gcggtgcgag ccgcaggcca gcatagcggc tgcgaaaaag gtgagcgtgc cgtacttcac
      541 gaggtgcagg aatgacatgg ggcgcaaggt accgggtccg gggaggcttg cgatcaacca
      601 agacgctgcg tcggacgaaa ggcatattcc cgcaacaacc cgcaattccc gcattggaag
      661 ctcacgatcg ggaacgcaag tttgccagcc aggtccagac cgcaccgaac gctgtccttc
      721 gaagtgcctt gcacccatac ggtcctgtcg gaaagtatcg cattccaact gtcgggtacg
      781 atcccgggtt ccatggggca tttgcagtcc gggcattccg gtatacgggg agccgggtat
      841 ctggatcgtg tgcgttcggt gctcatcctc ctgccttttt cgccttgtat cctttttccg
      901 tcaggtaggc cagcaccttg tcgcggtgat caccttgcag caggatcacg ccatccttgg
      961 tgttgccgcc cacgccgcac ttgctcttca gcgcgcggcc gagatcgtcc aggtccgcat
     1021 ccttgccggt gaagccgacg atccgtgtga cggtcttgtt gccgcccagg cggtccaggt
     1081 ggatgcgcag atcctgctgg ccgggagctg cggtcggttt gccggaacct tgtgggccgc
     1141 tcgaccaggc caattcaccg aacgaggtga gggttcgctt cttcatgacc gccgcaaagt
     1201 agcttgtcgc tgaacgttcc gaccttgtgc cgcgatgcga ggtgtggtca cagtgatccc
     1261 tgttcgtggt gcgaaagagc tggcgcactt tatccggctg ccgttccaac tgaaggacca
     1321 gcggaagaac cacgtaccgc ccttgctcgc ggacgagcgt gagttccacg acccgaagaa
     1381 gaacccgcaa ctggcgcatg gcgaagtgga gcgctggatc gcctggcgcg gtgatcaggc
     1441 ggtgggccgg atcatgggca tcatccaccg ctcgtacaac gcgaagcaga gcgaacgcac
     1501 agcacgcttc taccagctcg atagcgagcg cgatggggaa gtggtgcgcg ccttgatcgg
     1561 tgcggtggag gagtgggcac gttccaaagg catggaccgc atgatcggcc ccttcggctt
     1621 cagcgacaag gacccgcaag gcgtgcagat cgccggcttt gaacagctgc cggtgatcgc
     1681 cacgccgacc aatgctgatt ggctgccggc catggtcgag gcgtgtggtt acacgcagtt
     1741 cgaggacatg gtgagctacc gcatggatat tcctgcgcga cttccggagc gctatcacgc
     1801 catcgcggac cgctgtctgc ggaacctggg ttcgcatcgc gtgcctttgg gctccaagcg
     1861 tgagttgaag ccttgggtgg tgccggtgct gcggctggtc aatgccacat acggcgacct
     1921 gctcggcttc gcgccgatga gcgaggaaga gatgcacgta ctggcgaaga agtacatgta
     1981 catcctcgat ccgcaactcg ttcatctgat cgccgatgcg aagaacgagc ccgtggcctt
     2041 cgtgatcgcc agcccggaca tgagcgaggg tcttgtccga gcgaacggga agttgttccc
     2101 gttgggcttt gtgcatattc tgcgggccat gaagcgatcg aagcagctcg atctgctact
     2161 cggtgcggta cggccggatc tgcaaggcaa aggactcacg gccgcgctcg gcatttcgct
     2221 cttcgatgct gcgcgccaac atggcttcac gcacctggac agccacctga tcatggagcg
     2281 taacacgcgc atgcgctccg aactggagcg gctgggggcg gtggtgtgga agcggtaccg
     2341 ggtgtaccag cgcgggttga gttgaccaca gaggtacgga tggctcagag gattacaagt
     2401 tggcgtgcgg aggatgctgt tcccatgggc aagggcccag ggtcaactgt ccggatcctg
     2461 actttctccg tgtccccgga gcctctgtg
//
LOCUS       DAOQOC010000372         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24693_length_2484_cov_3.218608, whole genome shotgun sequence.
ACCESSION   DAOQOC010000372 DAOQOC010000000
VERSION     DAOQOC010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24693_length_2484_cov_3.218608"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PL106_06900"
     CDS             complement(<1..1244)
                     /locus_tag="PL106_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA32834.1"
                     /translation="MHIHIVVSRLILFELRFWLRQPLLWVFLGLAFAFGCAAAVFESG
                     GQGGPGMVHVNAPRHVYELYANLAFLWLPMVTAFVNATAIRDFACRTSEIVFSTVVTR
                     RQYLFGRFLGSTLVALIPTLGISAGLVTGSWIPYGDPIRFGPNSWAVHGQAFLWFAVS
                     SIAFQSAVMFAVATLVRSTAAAFLTSIVLVVLNGVAMAYATQVDNAWLASLLDPFGVN
                     ALDEVTRYWSVHDSNTALLPVTGALTWNRLLWLGIAAIVFVIGYLRFSFTGRRSRVKP
                     LAEGPSAALADPTIPVVAIVHDGRTRAKQFISMLRSDLSSILRSPVYWIIAGLGLVLC
                     LVALSEVSQAFGTRTWPVTYNITELLRGLSMLFIVVLITYYSGELFWRDRDVGLDGIL
                     HALPVRSRWRVPAHFTAVLVVGG"
     gene            complement(1306..>2484)
                     /locus_tag="PL106_06905"
     CDS             complement(1306..>2484)
                     /locus_tag="PL106_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNA32835.1"
                     /translation="RNPRRWKPNACSMNFYSFMSARYEVLREKWTDPADPSHQVDVEV
                     YYHPAHAENVPRMANSIHKSLSYYNAHFGPYRHKQARIIEFPRYQTFAQAFPGTMPYS
                     EGIGFIADLSDTTDIDMVFYVVAHEMGHQWWAHQVMGAKMQGATLLSESMAQYSALMV
                     MEKEYGRAAMRKFLKLESDRYQRSRGSEELKEVPLLQVENQGYIHYNKGSIILYGLRD
                     FVGEDSLNKAFRALVDTFAYAEPPYPTALDMYRELEKVVPDSLTYLLEDGLKHITLYN
                     NRVDTATARMLPDSTWEVSLQLWGEKNYADSLGRETPAPMNDWVDVSILRMPQFGSKA
                     DKSLNDVPLLQERVRLKSGKNTLTFIVQGKPMRAEIDRDHLFIDRVMEDNGKKVDLGQ
                     "
BASE COUNT          486 a          792 c          753 g          453 t
ORIGIN      
        1 cccccgacaa cgagcactgc cgtgaaatgc gccggcacgc gccagcgtga tcgcacgggc
       61 aacgcgtgca ggatgccgtc caggcccacg tcccggtcac gccaaaacaa ttcaccgctg
      121 taataggtga tgagcaccac gatgaagagc atggacaagc cccgcagtag ttcggtgatg
      181 ttgtacgtga ccggccaggt gcgggtaccg aacgcctggc tcacttcgct cagcgccacc
      241 aggcacaaga ccaggcccag cccggcaatg atccaataaa ccggtgatcg tagaatgctc
      301 gacaggtccg atcgcagcat gctgatgaac tgcttcgcac gtgtgcggcc gtcgtgcacg
      361 atcgccacca ccgggatggt ggggtccgca agagctgccg aaggcccttc agccaagggc
      421 ttcacacggc tgcgccgccc cgtgaaggag aagcgcaggt agcctatcac gaagacgatc
      481 gctgcgatgc cgagccacaa gaggcggttc cacgtcaatg cgccggtgac aggcaacaac
      541 gccgtattgc tgtcatgtac gctccagtag cgcgtcactt cgtccagcgc gttcacgccg
      601 aagggatcca gcaacgaggc gagccaggca ttgtccacct gggtggcgta cgccatggcc
      661 accccgttga gcaccaccag tacgatggac gtgaggaagg ccgcagccgt ggaccggacc
      721 agggtggcca cggcgaacat caccgcgctc tggaaggcga tgctgctcac cgcgaaccac
      781 aggaaggcct gcccatgcac ggcccaactg ttggggccga accgtatggg gtcgccgtat
      841 gggatccaac tgccggtgac cagccccgcc gaaatgccga gcgtggggat gagcgccacc
      901 agcgtgctgc cgagaaagcg tccgaacaaa tactgcctcc gcgtgaccac cgtgctgaac
      961 acgatctccg atgtgcggca ggcgaagtcg cggatggccg tggcgttcac gaaggccgtg
     1021 accatgggca accacaagaa ggccaggttg gcgtagagtt cgtacacatg ccgcggcgcg
     1081 ttcacatgca ccatgcccgg gccaccttgg ccaccgctct cgaacaccgc cgccgcacag
     1141 ccgaatgcga acgcaaggcc aaggaagacc cacagcagcg gctggcggag ccagaaccgc
     1201 agttcgaaga ggatgaggcg ggagaccact atgtgaatat gcaatgctca atgtccaagg
     1261 tcgtaaccga cctgatcact gaaccgaggt atcgaccatt acgttctact gccccagatc
     1321 caccttcttg ccgttgtcct ccatcacccg gtcgatgaac aagtggtcac ggtcgatctc
     1381 cgcgcgcatc ggcttgccct gcacgatgaa ggtgagcgtg ttcttcccgc tcttcaatcg
     1441 gacccgttct tgcaacaacg gcacatcgtt cagcgacttg tccgctttgc tgccgaactg
     1501 cggcatgcgg aggatgctca cgtcgaccca gtcgttcatc ggtgccggtg tctcgcggcc
     1561 caggctgtcg gcatagttct tctcgcccca gagctgcaac gaaacttccc aggtgctatc
     1621 gggcaacatg cgggccgtgg ccgtatccac acggttgttg tacagggtga tgtgcttgag
     1681 gccgtcttcc agaaggtacg tgaggctgtc cggcaccacc ttctccagtt cgcggtacat
     1741 gtccagcgcg gtgggatagg gtggctcggc ataggcgaag gtgtccacca acgcacggaa
     1801 ggccttgttc aggctgtcct cgcccacgaa gtcgcgcagg ccgtagagga tgatactgcc
     1861 cttgttgtag tggatgtatc cctggttctc cacttgcaac aagggcacct ccttcagttc
     1921 ctcgctcccg cgcgagcgct ggtaccgatc gctctccaac ttcaggaact tccgcatggc
     1981 cgcgcggccg tactcctttt ccatcaccat gagcgcgctg tattgcgcca tgctctcgct
     2041 cagcaaggtg gcgccctgca ttttggcacc catcacctgg tgcgcccacc actggtgccc
     2101 catctcgtgc gccaccacgt agaacaccat gtcgatgtcg gtggtgtcgc tcaggtcggc
     2161 gatgaagccg atgccttcgc tgtacggcat ggtgccgggg aaggcttgcg cgaaggtctg
     2221 gtagcggggg aactcgatga tgcgcgcctg cttgtgccgg tacgggccga agtgggcgtt
     2281 gtagtagctc agcgacttgt ggatgctgtt ggccatgcgc ggcacgttct cggcgtgcgc
     2341 cgggtggtag tacacctcca catccacctg gtgcgacggg tcagcgggat cggtccactt
     2401 ctcccgcagc acctcgtagc gcgcgctcat gaacgagtag aaattcatgc tgcacgcatt
     2461 cggtttccac cgcctaggat tccg
//
LOCUS       DAOQOC010000373         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24695_length_2484_cov_3.119802, whole genome shotgun sequence.
ACCESSION   DAOQOC010000373 DAOQOC010000000
VERSION     DAOQOC010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24695_length_2484_cov_3.119802"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PL106_06910"
     CDS             complement(<1..403)
                     /locus_tag="PL106_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNA32836.1"
                     /translation="MLTPLTAISPIDGRYHNKTAALSRWFSEYALIRSRLEVELAWFE
                     FLCTVPLPELKGTDLSRLEPLRTRITSLSEADAGRVKEIEHTTNHDVKAVEYWLKEGL
                     REAGLGMEKEFVHFALTSQDVNNTAVPLLLKD"
     gene            complement(484..1929)
                     /locus_tag="PL106_06915"
     CDS             complement(484..1929)
                     /locus_tag="PL106_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32837.1"
                     /translation="MTCRTRITGLFVLSALCSAAQNENGVWLFNNTMGVDFMTTPPDI
                     LTNYTGFWGNTAVICDGNGDLMLYSDDTRILNGQDQVLVNGDGLFNSIYTNTTQSSMI
                     LPWPGHPGSYYMIQPYPWNLNDPVAHTYYHRIDMNAGGGIGQVMEKNIILADSTGAYM
                     TAVLDSAGTGIWVILHHLNAPRFMAYHLDDSGLSTTPMITGIGIPVESTSPYQAIGMI
                     RPSPHGERLYLNKLYNNVLNNALIHQMDFNRTTGEVSNHITFEGPTSGYHGMEISPSG
                     QFLYYVTYVCDSGIASRLWQYDISSGDSATMHDSRALVYEAPITPGGCMGKIGNLALA
                     VDGRIYCAVSNAQFLGVINQPDLPAPACEYLHHGLYLDGDTSWMWLPNQMREYPIGIP
                     DVVGDHKGPATLRIRPNPSRDGCRITLPGTGIRHLSIVDATGRLVREIPCVAGQGEVR
                     MDRGDLRGGIYRVVARNGDGCVEAAGRVVFE"
     gene            complement(1948..2283)
                     /locus_tag="PL106_06920"
     CDS             complement(1948..2283)
                     /locus_tag="PL106_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32838.1"
                     /translation="MNYSISCANQTNCDIKMSAFVDVTGNNCGSPTLAGSQFLSTGTT
                     TTFSFTIPSGYEFCYYLMTQQPNSGGPYLQTSGGDPCAWNFTGVDLCNDGPPNVSGKG
                     NCTSARFSQ"
BASE COUNT          502 a          697 c          748 g          537 t
ORIGIN      
        1 aatccttcag caataaaggt accgccgtgt tgttcacgtc ctggctggtg agggcgaagt
       61 ggacgaactc cttctccatg ccgaggcccg cttcgcgcag cccttccttc agccagtact
      121 ccacggcctt cacgtcgtgg ttcgtggtgt gctcgatctc cttcacacgc ccggcgtccg
      181 cctcggaaag cgatgtgatc cgggtgcgca gcggctccaa ccggctcagg tcggtgccct
      241 tcagttcggg caggggcacg gtgcacagga actcgaacca cgccaattca acttccaacc
      301 gggatcggat gagcgcgtac tcgctgaacc agcgggacag tgcggcggtc ttgttgtggt
      361 accggccgtc gatgggggag atggctgtga gtggggtgag catagggagc gcaaaagtac
      421 cttgccgcgt atgcatgtgg atgccgaacg ttctgcccgg ctattgtgca gtgagggccg
      481 cccctactcg aacaccacac gtcccgctgc ttccacgcac ccatcgccgt tccgtgcgac
      541 aacccggtag atgccgccac gcagatcccc gcgatccatg cgtacttcac cctgcccggc
      601 aacgcacgga atttcgcgaa caagccttcc tgtcgcgtcc acgatcgaga ggtgccggat
      661 acccgtgccc ggaagggtga tgcggcaccc gtcgcgcgac ggattcggcc ggatgcgcaa
      721 ggtggccgga cctttgtgat ccccaaccac atcagggatc ccgatcgggt actcgcgcat
      781 ctggttcggc agccacatcc aggaagtatc cccgtccaga tacagaccat gatgcaggta
      841 ttcacaggcg ggagcgggca ggtcgggttg gttgatgacc ccgaggaatt gagcgttgga
      901 gaccgcgcag tagatcctgc catcaacggc gagcgcaagg ttgccaatct ttcccatgca
      961 accgcccggt gttatcggtg cttcgtacac cagggctcga gaatcgtgca tcgtggccga
     1021 gtccccggaa gaaatgtcgt attgccataa gcgggaggcg atccctgagt cgcagacata
     1081 ggtcacgtag tacaggaact ggccggaggg ggagatctcc atcccatgat agccggatgt
     1141 tggcccctcg aacgtgatat ggttggatac ctcgcccgtg gtccggttga agtccatctg
     1201 atgtatcagc gcattgttca ggacgttgtt gtaaagcttg ttcaagtaca agcgctcccc
     1261 atgcggagaa ggtcggatca tcccgatcgc ttggtagggt gaggtggact cgaccggaat
     1321 acctattccg gttatcatcg gcgtggtgga cagccccgaa tcatccaggt gataagccat
     1381 gaaccggggc gcgttcaagt gatgcaggat cacccagatg cctgttcctg ccgaatccag
     1441 cacggccgtc atgtaggcac cggtactgtc cgccagtatg atattcttct ccatgacctg
     1501 cccgatgccg ccacctgcgt tcatatcgat cctgtggtag tacgtgtgtg ctactgggtc
     1561 attcaggttc cagggatagg gctgtatcat gtaatacgac cccggatgtc ccggccaggg
     1621 aaggatcatg cttgattgtg tcgtattggt gtagatgctg ttgaagaggc catcaccgtt
     1681 cacaagtact tgatcctgtc cgttcaggat gcgcgtatcg tccgaataga gcatcagatc
     1741 gccattccca tcgcagatca cggcggtgtt gccccaaaag ccggtgtagt tggtgaggat
     1801 gtccgggggc gttgtcatga agtcaacacc catggtattg ttgaagagcc aaacaccgtt
     1861 ctcattctgc gctgccgaac acagtgcgga gagaacgaat agccccgtga tgcgggttct
     1921 gcaggtcatg ggtccgccgg tctcatgcta ttgactgaac cgggcactgg tgcaattgcc
     1981 cttgccggat acattgggag ggccatcgtt gcacaggtcc actccggtga aattccatgc
     2041 gcacggatcc ccgccgctcg tctgaagata tggcccgccc gaattgggct gttgggtcat
     2101 caagtagtag caaaattcat acccgctggg tatggtgaat gagaaagtcg tggtggtgcc
     2161 cgtggagagg aattgcgatc cagccaaggt gggtgagcca cagttgttcc cggtcacatc
     2221 aacaaaggcg ctcatcttga tgtcacaatt cgtctggttc gcgcagctga tggaatagtt
     2281 catctgcgct tgtatgatcg ggcaaagccc aagggcggat gcacacacaa gcaggggcac
     2341 gaggcgtcca tggagcaact gttcacatcc cgtgccattg gcatcgggcg atcggtcatt
     2401 cgtgctcacc ggatgtattt tgtacgaagt tactcagtcg gtggtcaaga tccggtttga
     2461 ccggcgatca accatggtgg gtcg
//
LOCUS       DAOQOC010000374         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24706_length_2484_cov_2.114450, whole genome shotgun sequence.
ACCESSION   DAOQOC010000374 DAOQOC010000000
VERSION     DAOQOC010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24706_length_2484_cov_2.114450"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PL106_06925"
     CDS             complement(<1..1409)
                     /locus_tag="PL106_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32839.1"
                     /translation="MSVKNLEERMKAAGGAIKMLRDSQTGPNPYPVVKPEYTNWRDEQ
                     WAWQNTAILFNQSFHMSDLFVSGPESFEFLQYHGINSFKGFEPGKAKQYVPVTWDGHV
                     IGDVILVYLAKNKFDLVGRPPVINWISYHAETGKNPKTGKKWQVKLVRDDRSADRKNP
                     WARKFFRFQIQGPNAMKVTTKLLKKAPPELKMFNWTYVKIAGKQVGALRHGMAGQPGY
                     ELFGPMKDYEAVHSAIVKAGEEFGLRLVGGRAYSSNTLESAWIPSPMPGIYSGDDKYV
                     KYRKWLKADCFEGNASLGGSYYSNNIADYYVTPFDLGYGFMIKYDHEFIGKAALEKIH
                     NNKHRIKVTLELVSADVQKVLASQYDTKGSPGERGKFFEYPSAVYSMYPQDKVLSKDG
                     SKQVGISTWVGYTSNERKELTLAYLEPEYAAEGTEVIFVWGEENGGSLKPTVERPHKQ
                     FQIRAKVCKAPYVQEVQKA"
     gene            1655..>2484
                     /locus_tag="PL106_06930"
     CDS             1655..>2484
                     /locus_tag="PL106_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA32840.1"
                     /translation="MATPATQLNATVTTALYIGGKERQTAEKMAIADPAKPGVVVGYA
                     ASATPQDVSDAVAAAKAAYPAWSALTPQQRAEKMKAAIEGVPDSPNPFASEDGAILSQ
                     ENGKIRLEGWVDALVLQIRWNLALMHADEVNAAKVLKPVPGHIPTETTVSYQPLGVCS
                     IIVPFNWPIAILGAALPHALLAGNTVIVKPPLTAPLATTRVVQRIAEKLPPGVLNVVT
                     GPDANMKALIQNPDIAKVCFTGSVGGGKKMMELASQSLTRVTLELGGNDAAILLKDAI
                     "
BASE COUNT          425 a          815 c          756 g          488 t
ORIGIN      
        1 taggccttct gcacttcctg cacgtacggc gccttgcaca ccttcgcgcg gatctggaac
       61 tgcttgtgcg gacgctcgac cgtcggcttc agcgagccgc cgttctcctc gccccacacg
      121 aagatcactt ccgtgccttc ggccgcgtat tccggctcga ggtaggccag cgtcagttcc
      181 ttgcgctcgt tcgaggtgta gcccacccag gtggagatgc ccacctgctt gctgccatcc
      241 ttcgacagca ccttgtcctg cgggtacatg ctgtacacgg ccgacgggta ctcgaagaac
      301 ttgccgcgct cgccgggact gcccttggtg tcgtactgcg aagcgagcac cttctgcacg
      361 tccgccgaca ccagttccag cgtcaccttg atgcggtgct tgttgttgtg gatcttctcg
      421 agcgcagcct tgccgatgaa ctcatggtcg tacttgatca tgaagccata gcccaggtcg
      481 aagggcgtga cgtagtagtc ggcgatgttg ttgctgtagt agctgccgcc cagcgaggcg
      541 ttgccctcga agcagtcggc cttcagccac ttgcggtact tcacgtactt atcgtcgccc
      601 gagtagatgc ccggcatcgg cgagggaatc cacgcggatt cgagcgtgtt cgaggaatac
      661 gcgcggccgc ccaccaggcg caggccgaac tcctcgccgg ccttcacgat ggcgctgtgc
      721 acggcctcgt agtccttcat cggtccgaac agctcgtagc ccggctgacc ggccatgcca
      781 tgacgcagcg cgccgacctg cttgccggcg atcttcacgt aggtccagtt gaacatcttc
      841 agttccggcg gagccttctt cagaagcttc gtcgtcacct tcatcgcgtt cggaccctgg
      901 atctggaagc ggaagaactt gcgcgcccag ggattcttgc ggtcggccga acggtcgtcg
      961 cgcaccagct tgacctgcca cttcttgccg gtcttcgggt tcttgcccgt ctcggcgtgg
     1021 tagctgatcc agttgatcac cggcggacgg ccgacgaggt cgaacttgtt cttggccaga
     1081 tacacgagga tcacgtcgcc gatcacgtgc ccatcccagg tcacgggcac gtactgcttg
     1141 gccttgccgg gctcgaagcc cttgaagctg ttgatgccgt ggtactgcag gaactcgaag
     1201 ctctcggggc cggacacgaa caggtccgac atgtggaagc tctggttgaa caggatggcg
     1261 gtgttctgcc acgcccactg ttcgtcacgc cagttcgtgt attccggctt caccaccgga
     1321 taaggattgg ggccggtctg cgagtcgcgc agcatcttga ttgcgccgcc agcggccttc
     1381 atgcgttctt cgagattctt gacgctcatg tcttctccgt gaggctgcgc tgatgaccgc
     1441 gcaggggtgt atttaaggtg cgtgtaacag gggccgggca tcttgtttta aggccccggc
     1501 gctgtcaatt tgcgattccg gcagcctctg cccgcggcag gtttcaacct attttttgcg
     1561 gggaaaaatg cctgcccact gccgtgaaaa acggcggtaa cctagggctt tcaggcatac
     1621 cgccgacgca ttaccgaaga ggggaatcac gtccatggca actcccgcca cgcagctcaa
     1681 cgccaccgtt accaccgccc tgtacatcgg gggcaaggaa cgccagaccg ccgagaagat
     1741 ggccatcgcc gatcccgcca agcctggcgt ggtggtgggc tacgccgctt ccgcaactcc
     1801 gcaggacgtc agcgacgccg tcgccgctgc caaggcagcg tatcccgcgt ggtcggcgct
     1861 gacgccgcag cagcgcgccg agaaaatgaa ggccgccatc gagggcgtgc ccgattcgcc
     1921 gaatcccttc gcctccgaag acggcgccat cctctcgcag gagaacggca agatccgcct
     1981 ggaaggctgg gtggatgcgc tggtgctgca gatccgctgg aacctggcgc tgatgcatgc
     2041 cgacgaggtg aacgcggcca aggtgctgaa gccggtgccg ggccacattc ccaccgaaac
     2101 caccgtcagc taccagccgc tgggcgtgtg ctccatcatc gtgcccttca actggcccat
     2161 cgccatcctg ggtgcggcgt tgccgcatgc attgctggcc ggcaacaccg tcatcgtgaa
     2221 gccgccgctc accgcgccgc tggccaccac gcgcgtggtg cagcgcatcg cggagaagct
     2281 gccgccgggc gtgctgaacg tggtcaccgg ccccgacgcc aacatgaagg cactgatcca
     2341 gaaccccgac atcgccaagg tctgcttcac gggctccgtt ggcggcggca agaagatgat
     2401 ggaactggcc tcgcagagcc tcacccgcgt cacgctggag ctgggcggca acgatgcggc
     2461 catcctgctg aaggacgcca tcat
//
LOCUS       DAOQOC010000375         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24870_length_2476_cov_2.763734, whole genome shotgun sequence.
ACCESSION   DAOQOC010000375 DAOQOC010000000
VERSION     DAOQOC010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24870_length_2476_cov_2.763734"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2453)
                     /locus_tag="PL106_06935"
     CDS             complement(<1..2453)
                     /locus_tag="PL106_06935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32841.1"
                     /translation="MQGHGHAHDEPPEPFRAHAYQVIFSGAQLAHGEGRSAQPHYENF
                     FLGNDPASWGTGCRVFGEVVLRGLYPGVDLRIDGSKGLKYEFIVAAGVDPSIIQMRYE
                     GQDDIAVRDERLLVRTSCGTIAEEAPKAWDERGIVPVSYQLNGMSVRFRLPNEHAGVL
                     VLDPELTFGSYTGSTADNFGFTATYDNDGALYGGGIVFGGGYPATTGVLDNSFSGGNI
                     DIGISKWSPDGNTLQWSTYIGGSDNETPHSLVVNDNDELFVLAVTGSFDFPVTPGSFD
                     GSFNGGTLIPVNNGFVNLLGGEGYGFANGTDIAVMRLSPDASTLLASTYVGGSGNDGL
                     NQSTALAYNYGDHFRGEIALDAQERPVVATSTQSTNMPVAPGAPQPAYGGGDLDGFLF
                     RLDPDLTALQLATYRGGAQGDSGYGVQFSSTGDIYLTGGTASAGLTMAGSPTQNSFGG
                     GVDGHIARYADNGQLLSSTFVGTASTDQCYFVQLDTDDDVYVVGQTHGSYPVTPGKYV
                     NPGSSQFIHKFSPDLSTSIWSTRIGNGNGTEDISPSAFLVSNCGQIYFSGWGGSVNSN
                     MLAAQSTTIGLPVTSDAYQSTTDGSDFYLMVLDPEASGLAYATFFGGATSMEHVDGGT
                     SRFDKDGNVYQAVCAGCGGHDDFPTTPGAWSQTNNSFNCNLGVFKFNLGQPVAQIGID
                     GPGYVCFPGSAQFTNNSIGGTSQVWDFGDGASSTDFAPEHTWDEPGTYTVYMVLSDST
                     SCLPNDTASITVDVIVPDTASIDPVGTVCVGQSVQLHAHGGDTYQWSPTTGLSDPTSP
                     DPTATPSGAITYTVTATDA"
BASE COUNT          416 a          743 c          826 g          491 t
ORIGIN      
        1 catgcatcgg tggcggtcac ggtgtacgtg atggctccgg acggggtggc cgtggggtcg
       61 gggctggtgg gatcgctcag gcccgtggtc ggggaccatt ggtacgtatc gccgccgtgc
      121 gcgtggagct ggaccgattg gccgacgcat accgtgccca cgggatcgat gctcgcggta
      181 tccggcacga tcacgtccac ggtgatggag gcggtgtcgt tcggcaggca gctggtgctg
      241 tcactcagta ccatgtacac ggtatacgtg ccgggttcgt cccatgtgtg ttccggggcg
      301 aagtcggtgc tgctggcacc atcgccgaag tcccacacct ggctggtgcc gccgatgctg
      361 ttgttggtga actgggcgct gcccgggaag cacacgtagc ccggtccgtc gatgccgatc
      421 tgtgcgacgg gctgaccgag gttgaacttg aacacgccca ggttgcagtt gaagctgttg
      481 ttggtctggc tccacgcccc cggggtggta gggaagtcgt cgtggcctcc gcaaccggcg
      541 cacacggcct ggtacacgtt gccatccttg tcgaagcgac tggtaccgcc gtccacgtgc
      601 tccatgctcg tggccccgcc gaagaaggtg gcgtacgcca atcccgatgc ctcggggtcc
      661 agcaccatca ggtagaaatc gctgccatcg gtggtcgatt ggtaggcgtc ggaggtcaca
      721 ggaaggccga tggtggtgct ctgggcggcc agcatgttgc tgttcaccga tccaccccag
      781 ccactgaagt agatctgtcc gcagttgctc accaggaaag cgctggggga gatgtcctcc
      841 gtaccgttgc cgttgccgat gcgcgtgctc cagatggacg tggagaggtc cggggagaac
      901 ttgtggatga actgggagct gcccgggttg acgtacttgc ccggggtcac cggatagctg
      961 ccgtgcgttt gtccgacgac gtatacatca tcgtcggtat cgagttggac gaaatagcac
     1021 tgatcggtgg aggccgtgcc gacgaaggtg gaggacagga gctggccgtt gtcggcgtac
     1081 cgtgcgatgt gcccgtcgac cccgccaccg aaggagttct gggtcgggct tccggccatg
     1141 gtgagcccgg cgctggcggt accaccagtg aggtaaatat cgcccgtgct gctgaactgc
     1201 acaccgtacc cgctgtcgcc ctgcgcacct ccacggtagg tcgccagttg cagcgcggtc
     1261 aggtccgggt cgagccggaa gaggaagccg tcgaggtcgc ccccgccgta cgccggttgt
     1321 ggggccccgg gggccacggg catgttggtg ctctgtgtgc tggtggccac cacgggtcgt
     1381 tcctgggcgt ccagcgcgat ctcgcctcgg aaatgatccc cgtaattata ggccagtgcg
     1441 gtgctctggt tcaggccgtc atttcccgag ccgcccacat aggtggaagc gagcagcgtg
     1501 ctggcgtccg gggaaagccg catcaccgcg atatcggtcc cattggcgaa gccgtagccc
     1561 tcgccaccca ggagattcac gaagccgttg ttcaccggta tcaatgtgcc accgttgaag
     1621 gatccgtcga aactgccggg ggtcacaggg aagtcgaacg aaccggtgac ggccagtacg
     1681 aacagctcat cgttgtcgtt caccaccagg ctgtggggtg tctcattgtc gctgccaccg
     1741 atgtacgtgc tccattgcag cgtgttccca tccgggctcc atttactgat gccgatgtcg
     1801 atgttgccgc cggagaagct gttgtcgagc acgccggtgg tggccgggta tccgccgccg
     1861 aaaacaatgc caccaccgta cagtgcgcca tcgttgtcat aggtggccgt gaagccgaag
     1921 ttgtccgccg tgctgccggt gtacgatccg aacgtgagtt cgggatccag gacgagcacg
     1981 ccggcatgtt cattcggtag ccggaagcgt accgacatgc cgttcaactg ataggatacc
     2041 ggaacgatgc cccgttcgtc ccaggccttg ggggcctcct cggcgatcgt gccgcagctg
     2101 gtgcgcacga gcagccgttc atcccgcacg gcgatatcgt cctgcccttc gtaccgcatc
     2161 tgaatgatgg acggatcgac accggctgca acgatgaatt catacttcaa tcctttgctg
     2221 ccatcgatgc gcaggtccac accgggatag agcccgcgca gcaccacttc gccgaagaca
     2281 cggcaacccg ttccccatga cgcaggatcg ttacccagga agaagttctc gtagtggggt
     2341 tgtgcactcc ttccttcgcc gtgggcaagc tgtgcgccgg agaagatcac ctggtaggca
     2401 tgcgcccgga agggttctgg cggctcgtca tgcgcatgtc cgtgcccttg catggggccg
     2461 ccttggtgaa gcacgt
//
LOCUS       DAOQOC010000376         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24909_length_2474_cov_3.103348, whole genome shotgun sequence.
ACCESSION   DAOQOC010000376 DAOQOC010000000
VERSION     DAOQOC010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24909_length_2474_cov_3.103348"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="pyrF"
                     /locus_tag="PL106_06940"
     CDS             <1..738
                     /gene="pyrF"
                     /locus_tag="PL106_06940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNA32842.1"
                     /translation="KHLLELEDPVFEFNRQIIAATQDLCVAYKPNLAFYESQGWQGWR
                     TLEKTMALIPSDVFTIADAKRGDIGNTARKYAEAFYDKLGVDAVTIAPYMGRDSVEPF
                     LGRPGKWGVVLGVTSNDGARDLQFLTVDGGEQRLFERVMEKAASWGSVEDTMFVVGAT
                     RAEVLAQARTAAPHHFFLVPGVGAQGGDLKAVLDAGLTADGGLLINSSRGILYAGKDA
                     DAIPAARRAALHMRDVMALKLRERGII"
     gene            complement(739..1641)
                     /locus_tag="PL106_06945"
     CDS             complement(739..1641)
                     /locus_tag="PL106_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32843.1"
                     /translation="MLRSFVPALLLLSSGVDAQQVQDSLFRPSFADPAFNPGTGPCIA
                     IDQGHHNFHTRSGRYQACARVLEADGFRVVDNDGAFTEKSLKNLDALVIANALNVSNT
                     RQWALPTPSAFTEKEIAIVKAWVEGGGGLLLIADHMPFAGAAQELAAAFGFTFSNCFA
                     VEDVERPLERFYRGAGLSDHLLTIGVDTVVTYTGSAFQLPAGASTLIALNDGYTLLEP
                     DTAWRFNESTRERKGAGWYQAAVVNQGRGRVAIFGEAAMFSAQLAGPQAEPTGMNMPE
                     ARDNVRFLRNVARWLTGPDEPRMK"
     gene            1738..>2474
                     /locus_tag="PL106_06950"
     CDS             1738..>2474
                     /locus_tag="PL106_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNA32844.1"
                     /translation="MAEPSGTNVLLDPSFPYREELLQFIWEQRLFDPLNLTSADGEPV
                     DVLKPGRLHKNTGPDLVDAQVRIGGQHWAGNVEVHVRSGEWYAHAHEKDPAYNNVVLH
                     VVYEHDMEVRTHAGVRVPAVELKDRIPFGRLETFKELMHAQAWVPCAPQFGQVAHDRF
                     PIWLERVLIERLERKCAEVEALHDRLKGDAMETFWHVLARGFGFKVNAEPFGMLAEAL
                     PLRVLLKYRDDPERTEALLFGQAGLLR"
BASE COUNT          484 a          744 c          772 g          474 t
ORIGIN      
        1 aagcatttgc tggagcttga agaccccgtc tttgagttca accgccaaat catagcggcc
       61 acccaagacc tttgcgtggc ctacaagcca aaccttgctt tctacgaatc gcagggctgg
      121 caaggatggc gcactttgga aaaaacgatg gcgcttattc caagcgatgt attcaccatc
      181 gctgatgcaa aacgtggcga catcggcaac acggcacgca aatatgccga ggccttctac
      241 gacaagctgg gcgtggacgc cgtgaccatc gcaccgtaca tgggacggga cagtgtggag
      301 cctttcctcg gccgaccggg gaaatggggc gtggtgcttg gtgtcaccag caacgatggc
      361 gctcgtgatc tccagttcct caccgtggac ggtggcgagc agcggctttt cgagcgcgtg
      421 atggagaaag cggcttcctg gggttcggtc gaggacacca tgttcgtggt gggcgctacg
      481 cgtgcggagg tattggcgca ggcccgtacc gccgcaccgc accatttctt tcttgttccc
      541 ggtgtgggtg cgcaaggagg ggacctcaag gccgtgctcg atgccggtct caccgccgac
      601 ggcggcctgc tcatcaacag cagccgcggc atcctgtacg cgggcaagga cgcggatgcc
      661 atacccgccg cacgccgtgc tgcactgcac atgcgcgatg tcatggcctt gaagctccgc
      721 gaacgcggca tcatctgatc atttcattct tggttcatcc ggtccggtca accatcgcgc
      781 gacgttgcgc aggaagcgga cattgtcccg cgcttccggc atgttcatgc ccgtcggctc
      841 tgcttgcggt cccgccaact gggcactgaa catcgcggct tcgccgaata tggccacgcg
      901 gcccctgcct tggttcacaa ctgcggcttg gtaccatccg gcacctttcc gctcccgcgt
      961 gctttcgttg aaccgccagg cggtatcggg ttccagcaag gtgtagccat cgttcaaggc
     1021 gatgagcgtg gacgccccag ctggcaattg gaacgcgctg ccggtgtagg tcacgacggt
     1081 atccacgccg atggtcagca ggtgatcgga tagtccggca ccgcggtaga aacgctccaa
     1141 cggacgctct acatcctcaa ccgcgaaaca gttgctgaag gtgaacccga acgccgccgc
     1201 cagttcctgc gcggccccag cgaagggcat gtggtccgcg atcaggagca ggccgccgcc
     1261 gccttccacc caagctttta cgatcgcgat ctccttttcc gtgaacgcgc tcggtgtcgg
     1321 gagcgcccat tgtcgagtgt tcgacacgtt cagggcattg gcgatcacca gcgcatcaag
     1381 gttcttcaag ctcttctcag tgaacgcccc gtcattgtcg accacgcgga atccatcggc
     1441 ctccaacacg cgtgcgcacg cctgatagcg cccggaccgc gtgtggaagt tgtgatggcc
     1501 ctggtcgatg gcgatgcacg gaccggtccc cgggttgaac gcggggtcgg caaaggaggg
     1561 cctgaacaaa ctgtcctgca cctgctgtgc gtccacgccg gacgaaagaa ggagcagtgc
     1621 aggcacgaat gaacggagca tgcgagaaat gtatctacct tcttcggcgg cggttgacgg
     1681 aaaaacggtc aatagggtca aatggttgga ttagtgagca aagtcagatc atacactgtg
     1741 gccgaaccaa gcggaacgaa tgtgctgctc gacccgtcgt tcccctaccg cgaggagctc
     1801 ttgcagttca tttgggaaca gcgcctcttc gaccccttga acctgaccag tgcagatggt
     1861 gagcccgtgg acgtgctcaa accgggacgc ctgcacaaga acacgggccc tgacctggtg
     1921 gacgcccagg tgcgcatcgg cggccagcac tgggccggta acgtggaagt acatgtccgg
     1981 agcggcgaat ggtacgcgca tgcgcatgag aaggacccgg cgtacaacaa cgtggtcctg
     2041 cacgtggtgt atgaacacga catggaggtc cgtacacatg ctggtgtgcg ggtgcctgcc
     2101 gttgaactca aggaccgcat cccgttcggg cggctggaga ccttcaaaga actgatgcac
     2161 gcacaggcct gggtgccttg cgcaccacaa ttcggacagg tggcccatga ccgcttcccg
     2221 atctggttgg agcgcgtgct catcgagcgt ctggaacgga agtgcgcaga agtggaagca
     2281 ctccatgatc gcctgaaggg ggatgcgatg gagaccttct ggcatgtcct tgctcgcggc
     2341 ttcggcttca aagtgaacgc cgagcctttc ggcatgctgg cggaagcact gccgttgagg
     2401 gtgctgctca agtaccgcga tgatcccgag cgcaccgagg cgctgttgtt cggccaggcg
     2461 ggcctgctac gcat
//
LOCUS       DAOQOC010000377         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24948_length_2472_cov_3.732313, whole genome shotgun sequence.
ACCESSION   DAOQOC010000377 DAOQOC010000000
VERSION     DAOQOC010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24948_length_2472_cov_3.732313"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PL106_06955"
     CDS             <1..967
                     /locus_tag="PL106_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32845.1"
                     /translation="TGTNPITIDVPLPGAVFPQHAVPAEGGDLVVCGSIIPSGRYDQD
                     ALLVRISAVGTLLWLWTSNDADGNEEFLSIDALEDGWAVGGEWRHGADSDGLLARFTS
                     NGEPSWQQHYGSANDERATGVAHDAGGLVIVGSNTFPVGNSQFDHDAWAIRTDLDGNQ
                     QWQITLGGAGEDQASDVAASGNGSFVWAGHTRTWPLDDTTANGERLAHAWLVAIDLFG
                     DTLWTRTFGDITFNRYAGTITVLPDGGDLLVAGAYGSGHRSEALVMRTGPTGDLQWER
                     TYTVQRSSLIRALTSLPDGGFAAAGKCTGPQGGQTLLMRKNGNGQ"
     gene            1059..>2472
                     /locus_tag="PL106_06960"
     CDS             1059..>2472
                     /locus_tag="PL106_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNA32846.1"
                     /translation="MIRTTILCCLFPAVLVAQDKATLDAVIATKRFPIPAMADGPTKT
                     STQLDLHISVLGNTAVWKANADGERQAHVEALSIIEKDTTIIDFRKSDILGPGRTDTL
                     VGDFLIEEHFVLPPGEYTLSVELHDLNGPADNHSVWKGPLVIPEPGKGVEFSDIMLTD
                     GTRQDAQGNTVPAPFSGTYYPPEAQRLGFYTEILGTPGTFGTGAPFIINYQIETYETH
                     SVKGGFKGTQRASAAPVVTLSKEFPIDQLPSGNYLLAVEVVGNDGAEVARQEQFFQRN
                     NPLKYDLNALESVDITSTFADAFTDPDTLAEHLACLRPIAQPLERRMIDERLKDRDMT
                     LMKRFFYTFWANRDGYDPERAWKSYHVQVVNANRMYGCRNLRGYESDQGITFLKYGLP
                     STVVDGSRDNKQLPYLIWHYYRAGRYSDKRFVFWEQNTGIGCWELLHSEIPGERHNPQ
                     WQAMLSAPLGNASMTDGTRVL"
BASE COUNT          504 a          766 c          787 g          415 t
ORIGIN      
        1 caccgggacc aacccgatca cgatcgatgt gcccctgccg ggtgcggtct tcccccagca
       61 tgccgtcccg gccgaaggtg gcgatctggt ggtatgcgga tcgatcatac ccagcggccg
      121 gtacgatcaa gacgcgctgc tcgtccgcat cagtgcggtt ggtacactcc tgtggctttg
      181 gaccagcaac gatgccgacg ggaacgagga attcctgtcc attgatgcgt tggaggacgg
      241 ctgggccgtg ggcggggaat ggcggcacgg tgccgacagc gatggtctgc tggcgcgatt
      301 caccagcaac ggcgaaccca gctggcagca gcactacggt tccgccaacg atgagcgggc
      361 caccggggtg gcgcatgatg cgggcggact ggtgatcgtg ggcagcaaca cctttccggt
      421 aggcaacagc cagtttgacc acgatgcctg ggcgatccgc accgacctgg acgggaacca
      481 gcaatggcag atcacactgg gtggcgctgg cgaggaccag gcctcggatg tggccgcatc
      541 gggcaatgga tccttcgttt gggcggggca tacgcgcacg tggccgctcg acgacaccac
      601 ggccaatggc gaacggttgg cgcacgcctg gctggtggcg atcgacctgt tcggggatac
      661 gctgtggaca cgcaccttcg gagacatcac cttcaaccga tatgctggca ccatcacggt
      721 tctgcccgat ggaggggacc ttcttgtggc cggcgcatac gggagcgggc atcgctccga
      781 agccttggtc atgcgcaccg ggccgacggg cgacctccaa tgggaacgca cgtacaccgt
      841 gcagcgcagt tcacttatcc gagcgctgac atccctgccc gatggtggct tcgcagcagc
      901 cggcaagtgt accggccctc agggaggtca aaccctgctt atgcgcaaga acgggaacgg
      961 gcaatgagcc cggagcgagg cggccagcac atgggagtgg tgtgttcggc acgaggaacg
     1021 gcaaggtggg ccgggagcgc gggcctatct ttgggagcat gatccgcacc accatcctgt
     1081 gctgcctgtt ccccgccgtg cttgtggcgc aggacaaggc caccctggac gccgtcatcg
     1141 ccaccaagcg gttccccatc cccgcaatgg ccgatggccc gaccaagacg agcactcaac
     1201 tggacctgca tatcagcgtg ctgggcaata cggccgtctg gaaagcgaac gctgatggtg
     1261 aacggcaagc tcatgtcgaa gcgttgtcca tcatcgagaa ggacacgacc atcatcgact
     1321 tccggaaaag cgacatcctg gggcccggac gtacggacac cctggtgggc gacttcctga
     1381 tcgaggagca ttttgtactt ccacctgggg agtacaccct gagcgtggag ttgcatgacc
     1441 tgaacggccc ggccgacaac cattcggtgt ggaaggggcc gctcgtcatt cccgaaccgg
     1501 gcaagggggt agagttctcg gacatcatgc tcaccgatgg gacgcggcag gacgcgcaag
     1561 gcaacacggt accggccccg ttcagcggta cgtactaccc gcctgaagcg caacgcctgg
     1621 ggttctacac cgagatcctg ggcactcccg gaaccttcgg tacaggggct cctttcatca
     1681 tcaactacca gatcgaaacg tacgagacac actccgtgaa aggaggcttc aaggggaccc
     1741 agcgtgcatc ggcggcgcca gtggtgaccc tgtcgaagga attccccatc gatcaattgc
     1801 catccgggaa ctacttgctg gccgtggagg tcgtagggaa cgacggtgcg gaggtcgccc
     1861 gtcaggaaca gttcttccag cgcaacaacc cgctcaagta cgacctgaac gcattggaaa
     1921 gcgtggacat caccagcacc ttcgccgatg cgttcaccga tccggacacg ttggccgaac
     1981 acctggcctg cctgcgcccc attgcccaac cactggaacg ccgcatgatc gatgagcggt
     2041 tgaaggaccg ggacatgacc ttgatgaagc gcttcttcta cacgttctgg gccaaccgcg
     2101 acggctacga ccccgaacgc gcctggaaaa gttaccatgt gcaagtggtc aatgccaatc
     2161 gcatgtacgg ctgccgcaac ctgcgcggct acgagagcga tcagggcatc accttcctga
     2221 aatacggcct gcccagtacc gtggtggacg gcagccgcga caacaagcaa ctcccctacc
     2281 tgatctggca ctactaccgg gcgggccggt attccgacaa gcgattcgtc ttctgggagc
     2341 agaataccgg gatcggttgc tgggagctgt tgcactcgga gatcccgggc gaacggcaca
     2401 acccgcaatg gcaggcgatg ttgagcgccc cgctggggaa tgccagcatg acggacggaa
     2461 cgcgcgtgct ct
//
LOCUS       DAOQOC010000378         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_24992_length_2470_cov_3.696066, whole genome shotgun sequence.
ACCESSION   DAOQOC010000378 DAOQOC010000000
VERSION     DAOQOC010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24992_length_2470_cov_3.696066"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PL106_06965"
     CDS             complement(<1..264)
                     /locus_tag="PL106_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNA32847.1"
                     /translation="MRYSVSELSDVIRDRRSIQPKDLGAREVQRDILERLVQNAVWAP
                     NHGMTQPWRFTVFSGEGRKRLSAFLGAEYTRLTPPEKFLPKKFE"
     gene            376..1413
                     /locus_tag="PL106_06970"
     CDS             376..1413
                     /locus_tag="PL106_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32848.1"
                     /translation="MRTRLLSTLLLVAAGLPALAQITITAADNLPAVGDTFLYNTAAY
                     AAPPAGGDDVTFDYSALTTTGTTEFHWLAPSAYSGAAAFPNADLAATNELDTFLYEVT
                     GVGLERVAEHQHIVIPFPPTVIDLDVAHSNSILDLPLPLSNDDVWSDPLEGTVTSDGS
                     TGTRTGLVQGEADAYGYILLPGGGAPVPVLRVRTNLQETIQIPIGNQMTPVSHKRMQH
                     DYYAPWLKMPILSVFTDTLNAGLTVTESGIRYLASAPVGLAEHMAADQGFGLFPNPMN
                     DVVTITFSSAPGTGALLEVLDALGRTVRSERMGNRTWLSLSLAGEEPGCYFVRLTDTQ
                     GKMSAQRLIKR"
     gene            complement(1668..2081)
                     /locus_tag="PL106_06975"
     CDS             complement(1668..2081)
                     /locus_tag="PL106_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32849.1"
                     /translation="MRTDYPAKVLLAWAEAIRGHAPLRDWLMKNGYPELGVFVFALHL
                     KDDARDWLMKNGHAHLMAVITAIEGNKTALSWLEHHGMAVLKQVALAGDGDEEAVRWL
                     IARGHKELARAALNMHEVKQRIEDDHHDPHKYAQD"
     gene            2148..>2470
                     /locus_tag="PL106_06980"
     CDS             2148..>2470
                     /locus_tag="PL106_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32850.1"
                     /translation="MRTVLLFITLLIATVGLPQSDGMDRREATARRLLANNKPYRAIS
                     LLNGAIDREDRPVFHALRADAYNRIGVYNKAHADARTALVGLPGDPDLLLQLAISEQG
                     LGQAD"
BASE COUNT          443 a          815 c          718 g          494 t
ORIGIN      
        1 ctcgaatttt ttcggcagga acttctccgg tggggtgagc cgggtgtatt ccgccccgag
       61 gaaggccgac agccgcttgc gtccctcgcc gctgaacacg gtgaatcgcc agggctgcgt
      121 catgccgtga ttgggcgccc acacagcgtt ctgcaccaac cgttccagta tgtcgcgttg
      181 tacctcacgc gcaccgaggt ctttgggttg gatgctgcgc cggtcgcgga tcacgtcact
      241 cagttcgctc acactgtatc tcatggggct gatgttgcgg cgcaaagaac tggcggagcg
      301 ggatccaggc agcgttccgg cccggaatgc cgtcatactt ttcgaccgcg aaccacaaac
      361 cttcaaaaaa cgcgcatgcg cacacgactt ctctctacac tccttctcgt ggccgccggg
      421 cttccggcct tggcacagat caccatcacg gccgccgaca acctccccgc tgtgggcgac
      481 accttcttgt acaatacggc cgcctatgcc gcaccgcctg cgggaggaga tgacgtgacc
      541 ttcgactaca gtgctttgac gaccacgggc accacggagt tccactggct cgcaccatcg
      601 gcatactctg gcgctgccgc cttccccaat gcggatctgg cggccaccaa cgaactggac
      661 accttcctgt atgaagtgac cggcgtcggc ctggagcgtg tggccgagca tcagcacatc
      721 gtcattcctt ttcctccgac cgtgatcgat ctggacgtgg cacactccaa ttcgatactg
      781 gacctgccct tgccgttgag caacgatgat gtgtggagcg atcccctgga aggcacagtg
      841 acctccgatg gctccaccgg cacgcgcacc ggacttgtgc aaggggaggc tgacgcctat
      901 ggatacatcc ttctgcccgg tggtggcgct cccgtacccg tactgcgcgt gcgcacgaac
      961 cttcaggaaa cgatccagat ccccattggg aaccagatga cccccgtaag ccacaagcgc
     1021 atgcaacacg actactacgc cccttggctc aagatgccca ttctctccgt gttcacggac
     1081 acgctcaacg ctggcctcac cgttaccgag agcgggatcc gctatctggc ctccgcacct
     1141 gtcggcctgg ccgagcacat ggcagccgac cagggtttcg ggctattccc caacccgatg
     1201 aatgatgtgg tcaccatcac cttcagcagt gcgccaggca caggtgcgtt gctcgaagtg
     1261 ctggatgcac tgggtcgcac ggtacgttcg gagcgcatgg gcaaccggac gtggctgtcc
     1321 ctctcactcg ctggtgaaga acccggctgc tatttcgttc gcctcaccga tacccaaggt
     1381 aagatgagtg cccagcggct gatcaagcgc tgaacggacc actcccgcgt agaagggccg
     1441 tccggtttgc gggcggccct tcgttcttca ccctggccac ggcgaacctt gtgcccatgc
     1501 ggggccagtg aaccagagat cacctcccgc cggaggatgg tcgctcgccc aacggaaagt
     1561 tgctaccgat ttttatggaa ttgctcactg gtacatgtgg tgatgcggag gaggcgctgg
     1621 gccgtatgga cgaattgacc aaggagcaca gcttcatcga tggcggttca gtcctgcgca
     1681 tacttgtggg ggtcgtggtg gtcgtcctcg atccgctgtt tcacctcgtg catattgagc
     1741 gcggcacgtg ccaattcctt gtggccccgg gcgatcagcc atcgcacggc ctcctcgtcg
     1801 ccatcgcctg ccagggccac ctgtttgagc accgccatgc catggtgctc cagccaggac
     1861 aatgcggtct tgttcccctc gatggcggtg atcacggcca tgaggtgcgc gtgcccgttc
     1921 ttcatcaacc agtctcgtgc gtcgtccttc agatgcagtg cgaacacgaa cacgcccagt
     1981 tcggggtacc cgttcttcat gagccagtcg cgcaggggcg cgtggccacg tatggcctcg
     2041 gcccaagcca gcaacacttt ggccgggtag tccgttcgca tggtgcctcg gccggtccta
     2101 cggatcacga ccgctcgtaa ggtgggagcg aaggtatctt cggcccgatg cgtactgtac
     2161 tcctgttcat caccttgctc atcgccacgg tgggcctgcc ccagagcgat ggcatggatc
     2221 ggcgcgaggc cacggcccgc aggctgctcg ccaataacaa gccttaccgc gccatcagct
     2281 tgctcaacgg tgccatcgac cgcgaggacc gtccggtgtt ccacgcgctg cgtgccgacg
     2341 cgtacaatcg catcggtgtg tacaacaagg cccacgccga tgcgcgtacc gcattggtcg
     2401 gactaccggg cgacccggac cttcttcttc aattggccat cagcgagcag ggcctgggcc
     2461 aagccgatag
//
LOCUS       DAOQOC010000379         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25155_length_2462_cov_3.159119, whole genome shotgun sequence.
ACCESSION   DAOQOC010000379 DAOQOC010000000
VERSION     DAOQOC010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25155_length_2462_cov_3.159119"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PL106_06985"
     CDS             complement(<1..790)
                     /locus_tag="PL106_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNA32851.1"
                     /translation="MIQRIKEETARRHADMIAWRRHLHAHPELSFAEHTTGAYIAEQL
                     RGLGIGVREGVAGTGVIGMVQGAGPGQGCICLRADIDALPIQEANTCDYRSVNDGVMH
                     ACGHDAHTAMVLGAGAVLHELRDRWSGTVHLLFQPGEEKIPGGATLVLNEDALRDPRP
                     TLIIGQHCTPELELGKLGFRAGPFMASSDELYVTVKGKGGHAAQPAKLIDPIMITAHL
                     LPRLKADFAERYGAASTPSLLAFGKVEALGATNVVFTTPSMSTAS"
     gene            complement(787..1155)
                     /locus_tag="PL106_06990"
     CDS             complement(787..1155)
                     /locus_tag="PL106_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA32852.1"
                     /translation="MEPVTVLILVAIGILAGLLSGFVGVGGGIIIVPMLMWALAYSQH
                     KAIGTSLGVLLLPVGILAVWNYHRAGNLDWKAALIIGGTFVLGAWAGSRLSLSLPPDT
                     VKKVFGGVMILAALKLIFGK"
     gene            complement(1166..>2462)
                     /locus_tag="PL106_06995"
     CDS             complement(1166..>2462)
                     /locus_tag="PL106_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32853.1"
                     /translation="APPPETIPEGTGNEVAATPAPAGKDTLLVTSLAFPEQRNYNVNF
                     ATDEVLTQVDNSYDGRFYQSLTGNLNMNPGLSGYTRMAMSDLFEDYKISGGFRLALDL
                     NNNDYFLRLQNIRHRLDKDLVVQRRAIQGVVENAGLAKLHTHMATFRLTWPFSELTSL
                     RGSVMYRHDRAVIQSTDNLSLRKPNAYDQTVGGKLEYVYDSSIPRGLNLWTGWKAKVF
                     GEYYKQIDGQRTDMQVLGLDVRHAMRLHRELTWVTRLAGSTALGSRRVLFTLGGVDNW
                     LFPKVNNSTPIDGSQNYFYQSLAVPMRGFWYNTRNGNSFAVANTEVRVPIFRYLMNRP
                     IRSDLINNFQVVAFADAGTAWTGAHPYAEDNSFNNTTIVNGPYTITIKNLHEPIVWSY
                     GVGLRTRLLGYFVRADWAWGIDDGRQMPRVFQLSLNLDI"
BASE COUNT          434 a          769 c          776 g          483 t
ORIGIN      
        1 tgctcgcggt cgacatcgat ggcgtggtga agaccacgtt ggtcgcaccg agcgcctcca
       61 ccttgccgaa ggccagcaac gacggtgttg atgccgcacc atatcgctcg gcgaaatcgg
      121 ccttgagccg gggcagcagg tgcgccgtga tcatgatggg atcgatcagc ttggccggtt
      181 gtgccgcatg gccaccttta cctttcaccg tcacgtacag ctcatcgctg ctggccatga
      241 acggcccggc ccggaacccg agtttgccca gctccaactc aggggtgcaa tgctgtccga
      301 tgatcagcgt tgggcgcggg tcgcgcagcg catcctcgtt cagcaccaac gtggcaccac
      361 cgggtatttt ttcttcgccc ggttggaaaa gcaggtgtac ggtgccactc caacgatcgc
      421 gcaattcgtg cagcacggca ccggcaccca gcaccatggc cgtgtgcgca tcgtggccgc
      481 atgcgtgcat gacgccgtcg ttcacgctgc ggtagtcgca ggtgttcgcc tcttggatcg
      541 gcagcgcgtc gatgtctgcc cgcaagcaga tgcaaccctg gcccggaccg gcaccctgga
      601 ccatgccgat gaccccggtg ccggccacgc cttcacgcac cccgatgccg agtccacgca
      661 gttgctctgc gatgtacgcc cctgtggtgt gctccgcgaa gctcagttcc ggatgcgcgt
      721 gcaagtgcct gcgccaggcg atcatgtcgg cgtggcgccg tgccgtttcc tccttgatcc
      781 gctggatcat ttgccgaaga tgagcttcag cgcggccagg atcatcacac cgccgaagac
      841 cttcttcacc gtgtccggtg gaaggctgag cgagaggcgg ctgccggccc aggcacccag
      901 cacgaatgtg ccaccgatga tcagggccgc cttccaatcg aggttgcccg cgcggtgata
      961 attccagacc gccaggatgc ccacagggag cagcagtaca cctaaactgg taccaatggc
     1021 cttgtgctgg gagtaggcca gggcccacat cagcatgggc acgatgatga tgccgccacc
     1081 cacgcccacg aaaccgctga gcaggcccgc caggatgccg atggccacca ggatcagcac
     1141 ggtgacgggt tccatcggtg gctggtcaga tgtcaaggtt cagggacagt tggaacacgc
     1201 gcggcatctg ccggccatca tcgatgcccc aggcccaatc ggcccgcacg aaatagccga
     1261 gcaaccgcgt gcgcagaccg acgccgtaac tccatacgat gggttcgtga agattcttga
     1321 tggtgatggt gtacggtccg ttgacgatgg tcgtgttgtt gaacgagttg tcctcggcgt
     1381 acgggtgcgc gccggtccat gccgtgcccg catcggcgaa ggccaccacc tggaagttgt
     1441 tgatgaggtc gctgcggatc ggccggttca tcagatacct gaaaatgggc acgcgcacct
     1501 cggtgttcgc cactgcgaag ctgttcccgt tgcgggtgtt gtaccagaat ccgcgcatgg
     1561 gaacggccaa cgactggtag aaatagttct gcgacccgtc gatgggggtg ctgttgttca
     1621 ccttggggaa gagccagttg tccacaccac cgagggtgaa gagcacgcgg cgcgaaccca
     1681 gcgcggtgga acccgccaag cgggtgaccc acgtgagctc gcggtgcagg cgcatggcat
     1741 ggcgtacgtc cagtccgagc acctgcatat cggtccgctg cccgtcgatc tgcttgtaat
     1801 actcgccgaa gaccttggcc ttccaaccgg tccagaggtt cagcccgcgt ggtatgctgc
     1861 tgtcatacac gtactccagt ttgcctccca cggtctgatc ataggcgttc ggcttccgga
     1921 gcgaaaggtt gtccgtgctt tggatcaccg cgcgatcgtg ccggtacatg accgacccgc
     1981 gcaaactcgt cagctcgctg aacggccatg tgagcctgaa cgtggccatg tgcgtgtgca
     2041 gtttggccag gcccgcattc tccaccacgc cctgtatagc tcgtcgctgc acgaccaggt
     2101 ccttgtccag cctgtgtctg atgttctgca ggcgcaggaa atagtcgttg ttgttcaggt
     2161 cgagtgcgag gcggaacccg cccgagatct tgtagtcctc gaacaggtcg ctcatcgcca
     2221 tgcgggtgta gccgctgagg cccgggttca tgttcaggtt gcccgtgagc gactggtaga
     2281 agcgcccatc gtagctgttg tccacctggg tgagcacctc atcagtggcg aaattcacgt
     2341 tgtagttgcg ctgctcggga aaggcgaggc tggtcaccag aagggtgtcc ttcccggccg
     2401 gagcgggtgt tgcggcgacc tcgttgccgg taccctccgg tatggtctcc ggtggcggag
     2461 cc
//
LOCUS       DAOQOC010000380         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25182_length_2461_cov_2.917290, whole genome shotgun sequence.
ACCESSION   DAOQOC010000380 DAOQOC010000000
VERSION     DAOQOC010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25182_length_2461_cov_2.917290"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PL106_07000"
     CDS             complement(<1..227)
                     /locus_tag="PL106_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32854.1"
                     /translation="MRWRFADLPVRTKLLITLGIPVLGLVLLIGKQVDGSLKRRDVLG
                     YVRKQSGNIRLLGDMADALQEEGALSVAWLEG"
     gene            279..1838
                     /locus_tag="PL106_07005"
     CDS             279..1838
                     /locus_tag="PL106_07005"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNA32855.1"
                     /translation="METTTPTNSTFATLEEKARQALLGGGQERINAQHKKGKLTARER
                     VHLLLDEGSFQEIGQLVTHRSTNFGLGDQVFLGDGVVTGYGTIDGRLVYVFSQDFTVL
                     GGSLAEAHAEKICRIMDLAMKNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNVRAS
                     GVVPQISAIMGPCAGGAVYSPALTDFVMMVEGSSYMFVTGPNVVKTVTHEEVSAEELG
                     GASTHASKSGVAHFTAPNELEAIRELRRLVSFIPANCEEEPPALPYEASDESRPELDT
                     IIPENPNQPYDIRQVMNAVIDPDSSMEVHAEYARNMVIGFARVEGRTVGCVANQPAVL
                     AGVLDIDASIKAARFVRFCDAFNIPLLVFVDVPGFLPGTDQEWRGIIDHGAKLLYAFS
                     EATVPRITVITRKAYGGAYDVMNSKHIGCDMNYAWPSAEIAVMGAKGAAEIIFKNEIG
                     KAADKDAKWKEKEAEYKDQFANPYEAAARGYVDEVIRPSETRIKVIHALRMLRDKVDH
                     MPKRKHGNIPL"
     gene            1925..>2461
                     /locus_tag="PL106_07010"
     CDS             1925..>2461
                     /locus_tag="PL106_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNA32856.1"
                     /translation="MILKTSLLVAAALLAVGAHDPLKDLNIGDALPAADIAMKDVMGG
                     DKSLSALKGESGLLVIFSCNTCPFVIGSEGSTGWENRYPELGNFCRTNKIGFALVNSN
                     STKREAGDGFADMQAHYKEKNYNCHYLLDNDNIVADAFAARTTPHVFLFDKDLKLVYK
                     GAIDDNVADPKAVTKNYLK"
BASE COUNT          519 a          826 c          740 g          376 t
ORIGIN      
        1 ccctccagcc acgccacgga gagcgcaccc tcctcctgta gcgcatcggc catgtcgccc
       61 agcagccgga tgttccccga ttgcttgcgc acatagccca gcacgtcccg ccgtttcagg
      121 ctgccgtcca cctgcttgcc gatgagcagc accaagccga gcaccgggat gcccagcgtg
      181 atcaacaact tggtacggac cggcaggtcc gcgaaacgcc agcgcatcag tccggcgaat
      241 gtagggggga ggatatcgtc gcacctacat tcggcgcgat ggagaccacc accccgacca
      301 acagcacgtt cgccacgctg gaagaaaagg cccgccaagc cctccttggc ggcgggcagg
      361 aacgcatcaa tgcgcagcac aagaaaggca agctcaccgc gcgtgagcgt gtccacctgc
      421 tgcttgatga gggcagcttc caggagatcg gccagctcgt cacccaccgc agcaccaact
      481 tcgggctggg cgaccaggtc tttttgggag acggggtggt cacgggctac ggtaccattg
      541 acggccgcct cgtgtacgtg ttctcccagg atttcaccgt gctgggcgga tcgctcgccg
      601 aagcccatgc cgagaagatc tgccgcatca tggacctggc catgaagaac ggagctcccg
      661 tgatcggcct caacgacagc gggggcgcac gcatccagga gggcgtggtg agcctgggcg
      721 gatacgcgga catattctac cggaacgtac gggccagcgg cgtggtcccg cagatcagcg
      781 ccatcatggg gccctgcgcg ggcggcgctg tgtattcccc agcactcacc gacttcgtga
      841 tgatggtgga gggctccagc tacatgttcg tcacaggacc caacgtggtg aagaccgtta
      901 cccacgaaga ggtatcggcc gaggaactcg gaggggcttc aacccatgcc agcaagagcg
      961 gcgtggcgca cttcaccgca cccaacgagc tggaggccat ccgcgagctg cgacggctgg
     1021 tgtccttcat ccccgccaac tgcgaggagg aaccgcccgc cctgccgtac gaggcaagtg
     1081 atgaatcgcg gcccgaactg gacacgatca tccccgagaa cccgaatcag ccgtacgata
     1141 tcaggcaggt gatgaacgcg gtgatcgacc cggacagcag catggaggtg catgccgagt
     1201 acgcccgcaa catggtcatc ggcttcgccc gcgtggaggg ccgcaccgtg ggctgcgtgg
     1261 ccaaccagcc cgccgtgctg gccggtgtgc tcgacatcga cgcgagcatc aaggccgccc
     1321 gcttcgtgcg tttctgcgac gcgttcaaca tccccttgct cgtcttcgtg gacgtgcccg
     1381 gcttcctgcc cggcaccgat caggagtggc gcggcatcat cgaccacggt gcgaaactcc
     1441 tctacgcctt cagcgaggcc accgtgccgc gcatcaccgt gatcacccgc aaggcgtacg
     1501 gtggcgccta cgacgtgatg aacagcaagc acatcggctg cgacatgaac tacgcgtggc
     1561 ccagcgcgga gatcgccgtc atgggcgcga agggcgccgc cgagatcatc ttcaagaacg
     1621 agatcggcaa ggcggccgac aaggacgcga aatggaagga gaaggaggcc gaatacaagg
     1681 atcaattcgc caacccgtac gaggccgccg cgcgcggata cgtggatgag gtgatccgcc
     1741 ccagcgaaac acgcatcaag gtgatccacg ccctgcgcat gctgcgcgat aaggtggacc
     1801 acatgcccaa gcgcaagcac gggaacatcc cgttgtaggt ggcacgggat ccccaataaa
     1861 gccgaatatg cgacggccga agtccggcca cggccctact tttcggcccg ataaccccgc
     1921 acccatgatc ctgaaaacct ctctgctcgt cgccgccgcc ctgttggccg tgggagccca
     1981 tgacccgctc aaagacctca acatcggtga cgccctgccc gcagcggaca tcgccatgaa
     2041 ggatgtgatg ggtggcgaca agtccctctc cgccctcaag ggcgagagcg gcctgctcgt
     2101 gatcttcagc tgcaacacct gccccttcgt gatcggctcc gagggcagca ccggctggga
     2161 gaaccgctac cccgaactcg gcaacttctg ccgtaccaac aagatcggtt ttgccctggt
     2221 gaacagcaac tccaccaagc gggaggccgg tgacggcttc gccgacatgc aggcgcacta
     2281 caaggagaag aactacaact gccactacct gctcgacaac gacaacatcg tggccgatgc
     2341 cttcgccgcc cgcaccacac cgcacgtctt cctcttcgac aaggacctga agctggtgta
     2401 caaaggcgcc atcgacgaca atgtggccga tcccaaagcc gtgaccaaga actacctgaa
     2461 g
//
LOCUS       DAOQOC010000381         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25202_length_2460_cov_3.129314, whole genome shotgun sequence.
ACCESSION   DAOQOC010000381 DAOQOC010000000
VERSION     DAOQOC010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25202_length_2460_cov_3.129314"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..971)
                     /locus_tag="PL106_07015"
     CDS             complement(21..971)
                     /locus_tag="PL106_07015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA32857.1"
                     /translation="MLFNSIDFAVFLPLVFLGYWLIGPGRIRGQNAFLILAGCVFYGW
                     WDWRYLGLLLFTSGVDFLVALGLQREQATARRKLLLGISLAANLGLLGFFKYCDFFIT
                     AFNDGFTLLGRPLGLRTLGIILPVGISFYTFQSLSYTIDVYRRQLPATRDPLVFFAFV
                     SFFPQLLAGPIERARNMVKQFENPRTFDLAHAQDGLRQILWGLFKKVVVADNCGFLVD
                     PAYDDPTGHNGATLVLATIFFSFQIYCDFSGYSDMALGCARLFGFTLMRNFAYPYFSR
                     DMGEYWRRWHMSLNTWFRDYLYIPLGGSRGGKWMVLRVVG"
     gene            complement(1046..1237)
                     /locus_tag="PL106_07020"
     CDS             complement(1046..1237)
                     /locus_tag="PL106_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32858.1"
                     /translation="MTTSTVVHARIRGVTTERPGPLEWATSPKNGVGKARFALNSTYD
                     VRECRTIPAGAQATARTVE"
     gene            1336..>2460
                     /locus_tag="PL106_07025"
     CDS             1336..>2460
                     /locus_tag="PL106_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNA32859.1"
                     /translation="MSTPTFLKWLHEVELKDIPTVGGKNASLGEMIQHLGKLGVQVPG
                     GFVVTVASYEAFIAHNELDQKIHDIVAGLDTDDVENIRRTGLAVRTLIKNGKFPEEIW
                     KGIMARYDELSQKYGQDATDVAVRSSATAEDLPDASFAGQQETFLNIRGHQDLISAVR
                     NCFASLWTDRAIVYRQSLGYDHFNVGISVGVQKMVRSDLGASGVAFSLDTESGFRDVV
                     LINGSYGLGEMVVQGAVSPDEFLVFKPTLAEGYSAIIEKKIGNKDRKMIYGVEPGKPT
                     LTIPTERAMRNRFCISDDQALEIARGVTAIEKYYSAKKGLWCPMDVEWAVDGLSKQLF
                     IVQARPETIHSRKATDRVVEYRLDASAAPGGASPEVIAKGV"
BASE COUNT          537 a          758 c          752 g          413 t
ORIGIN      
        1 aactaactgg ttccctagcg ttaaccaacg actcgcaaca ccatccactt gccgccgcgg
       61 ctgccgccca acgggatgta gaggtaatcc ctgaaccagg tgttgaggct catgtgccag
      121 cgccgccagt actcgcccat gtcgcggctg aaatacggat aggcgaagtt gcgcatgagg
      181 gtgaatccga agagccgggc gcagccgagg gccatgtccg aataccccga gaagtcgcag
      241 tagatctgga aggagaagaa aatggtggcc agcaccagtg tggcgccgtt gtggcccgtg
      301 gggtcatcgt acgcgggatc caccaggaaa ccacagttgt cggccacgac cacctttttg
      361 aacaggcccc aaaggatctg ccgcaggcca tcctgcgcat gggccaggtc gaaggtcctc
      421 gggttctcga actgcttgac catgttgcgg gcccgttcga tcggcccggc gagcaactgg
      481 gggaaaaagc tgacgaacgc gaaaaatacc agcggatcgc gcgtggccgg aagctgcctg
      541 cggtacacat cgatggtgta actcaagctt tgaaaggtat aaaaactgat gcccaccggg
      601 aggatgatgc ccagggtacg cagaccaagc ggcctcccca gcaaggtgaa tccatcgttg
      661 aacgcggtga tgaagaagtc gcagtacttg aagaagccga gcaacccgag gttggccgcc
      721 aggctgatgc cgagcagcag cttgcgccgt gccgtggcct gctcgcgctg cagtcccaat
      781 gcgaccagga aatccacccc tgatgtgaag agcagcagac cgaggtaacg ccagtcccac
      841 caaccataga acacacagcc tgccaggatc aggaaggcgt tctgaccccg gatacgaccc
      901 ggcccgatca gccagtaccc gagaaagacc agcggcagga agaccgcgaa atcgatcgaa
      961 ttgaacagca tgcggaacta caaggatccg gcgctacgga atgggccgtt gataccgtgg
     1021 aagcggaata tccacgctcc ggtgttcact cgaccgtgcg ggccgtggcc tgtgcaccag
     1081 cgggtatggt cctgcattcg cgtacatcat aggtgctgtt cagggcaaaa cgggccttgc
     1141 ccaccccatt cttcggggag gtggcccact ccaacggtcc tggacgctcc gttgttactc
     1201 cacggatccg tgcatgaacc actgtggagg tcgtcatccg ggatcatcga tccatgcgca
     1261 cctttgtgtc ggcccagtgt atcgtcctga cccgttccgc gtacaagcgt cctcttctca
     1321 ccacacacca tctgcatgag caccccgacc ttcctcaagt ggcttcacga agttgaactg
     1381 aaggacatcc cgaccgtggg cggcaaaaac gccagcctcg gcgagatgat ccagcacctg
     1441 ggcaagctcg gcgttcaggt gccgggaggc ttcgtggtca ccgtggccag ttacgaggcc
     1501 ttcatcgcgc acaatgaact ggaccagaag atccacgata tcgtggccgg gttggatacc
     1561 gatgatgtgg agaacatccg acgcaccggc ctggccgtgc gcacacttat caagaacggg
     1621 aagttccccg aggagatctg gaagggcatc atggcccggt acgacgagct gagccagaaa
     1681 tacggccagg atgccaccga tgtggccgtg cgcagcagtg ccaccgccga ggacctcccc
     1741 gacgccagct tcgctgggca gcaggagacc ttcctgaaca tccggggcca ccaggacctg
     1801 atcagtgccg tgcgcaactg tttcgcctcg ctgtggaccg atcgcgccat cgtgtaccgc
     1861 cagagcctgg gttacgacca cttcaatgtg gggatcagcg tgggtgtgca gaagatggta
     1921 cgctccgatc tgggcgccag cggcgtggcc ttcagcctgg ataccgaaag cggtttccgg
     1981 gacgtggtac tcatcaacgg cagctacggc ctgggcgaaa tggtcgtgca gggcgccgtg
     2041 agtcccgatg agttcctggt cttcaagccc accctggccg agggctacag cgccatcatc
     2101 gagaagaaga tcggcaacaa ggaccgcaag atgatctacg gtgtggagcc cggcaagccc
     2161 acgctcacca tccccaccga gcgcgccatg cgcaaccgct tctgcatctc cgacgaccag
     2221 gccctggaga tcgcacgcgg cgtgacggcc attgaaaaat actactccgc caagaagggc
     2281 ctctggtgcc ccatggacgt ggaatgggcg gtggacggcc tgagcaagca gctcttcatc
     2341 gtgcaggccc ggccggagac catccacagc cgcaaggcca ccgatcgcgt ggtggagtac
     2401 cgcttggacg cctcggccgc tcccggtggt gcatcgcccg aggtgatcgc caagggtgtt
//
LOCUS       DAOQOC010000382         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25280_length_2456_cov_3.688047, whole genome shotgun sequence.
ACCESSION   DAOQOC010000382 DAOQOC010000000
VERSION     DAOQOC010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25280_length_2456_cov_3.688047"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PL106_07030"
     CDS             <1..1051
                     /locus_tag="PL106_07030"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32860.1"
                     /translation="AVRGNQLVVLGYCHSSDAYTWLQDGMEGGVPISNSCSATPGYLC
                     ICDPGGTAYHDEDAESTDDAYIAEYDPVQGTLSWSTYVGGNTWEYPGVYLMQLNSFSD
                     EGPFFFRKVADLRVDASGNVWAMTTTVNPHGDWQETPVPPAPPFYHKELDWTAGKFQS
                     EVTLHLFRPDHSLFYGTQFGAWFLHSESSAFNLLPFGCDIGKDLALVEGEAIYWTGTT
                     GNDRFPTQCPYPGTSYCEPFVESNNRYMQAFATRMSLQDINIGINDPLNLGPASLAVY
                     PNPTSAELHLFHQGLPLTAGLLSIIDATGRVVRGEPLAGSVVSVHGLANGIYHVQVQN
                     RTGRILGSASFVITR"
     gene            1067..>2456
                     /locus_tag="PL106_07035"
     CDS             1067..>2456
                     /locus_tag="PL106_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32861.1"
                     /translation="MRTTIWALPWIIPTVGCTQYFAPLGPGFDWGQAWDMLPVDGRLY
                     MAGDFAGTADSSVNSRGVLSWDGASFEAVGCGLFSCDLPQTWHMSAPARSLAYWQDEL
                     YIAGDFNLSSIPGVAFIARFNGNEWVGVGGGMDGPVRGLKAYPDGLYAAGWFNNAGNT
                     PAEGLARWDGEQWHSVHDLPLLDDYPINMVNDVAIINDRIYIGGNFSGTPQDSIYSIA
                     QWDGTTWNNLDHGFVGGLTQILKLEVHDSLLYIAGAFADTGQYGHPLNPASGIIAWDG
                     EDWVELGSGTDGANIPWVTDMAWHNDTLYVCGDFNRIGGVEAGGLARWDGQQWCALLP
                     SPPGAYGISCLTFFNDTLHLGGGLVYMGSDSVNRVAKWIGGNHVEECEEPVGIPQQVD
                     NEVPGITLYPNPATVELNVLHQGRPLPSGLLRITDTIGRSVAERPLTGSAVPLGPLAE
                     GVYHAQVTTEQGR"
BASE COUNT          478 a          726 c          739 g          513 t
ORIGIN      
        1 ggccgtgcgc ggtaaccaat tggtggtact cggctattgc cactcctccg atgcatatac
       61 ctggctacag gacggtatgg agggtggcgt gcccatcagc aatagctgtt cggctacccc
      121 tggctacctg tgtatctgcg atcccggcgg taccgcgtac catgatgaag atgccgagag
      181 cacggacgat gcgtacattg ccgaatacga ccccgtgcag ggcacattgt cgtggagcac
      241 atacgtgggc ggcaatacct gggaataccc gggcgtgtat cttatgcagc tgaattcatt
      301 cagtgatgag ggacccttct tcttccgcaa ggtcgccgac ctgagggtgg atgcttcggg
      361 gaacgtatgg gccatgacca ccaccgtgaa cccgcatggc gattggcagg aaacgcccgt
      421 accgcccgca ccgccattct accacaagga actggactgg accgccggga aattccagtc
      481 cgaggtgacc ctccaccttt tccggcccga ccacagcctc ttctatggga cgcaattcgg
      541 cgcctggttc ctgcattcgg aatcgagtgc cttcaacctg cttccgttcg gctgtgatat
      601 tggcaaagac ctggcgttgg tggaaggcga ggccatctac tggacgggca ccacgggcaa
      661 cgaccgtttc cccacgcaat gcccttaccc cggcacttcc tattgcgaac cattcgtcga
      721 atcgaataac cgatacatgc aggccttcgc cacgcgcatg agcctacagg acatcaacat
      781 cggcatcaac gatccactaa accttggtcc ggcttccttg gcggtatacc ccaatcccac
      841 cagtgcggaa ctccacctgt tccatcaggg gctgcccttg acagcgggcc tgttgagcat
      901 cattgatgct accggacgcg tagtaagggg ggagccgctc gccggctccg tagtgtccgt
      961 gcacggcttg gccaacggca tctaccatgt acaggtgcag aaccgtaccg gtcggatcct
     1021 gggttccgct tcgttcgtca tcactcgctg agccgtgaga ataccgttgc gcacgaccat
     1081 ctgggcgttg ccctggatca tccccaccgt gggttgtacc cagtacttcg cgccgctggg
     1141 gccgggcttt gattggggac aggcgtggga catgctaccg gtggatgggc ggctctatat
     1201 ggctggcgat ttcgccggta cagcggactc gtccgtcaac tccagggggg tcctatcttg
     1261 ggatggtgcc tccttcgaag ccgttggctg cgggctcttt tcctgcgatc ttccccaaac
     1321 atggcacatg agcgcgcccg cgcgatcgct cgcatactgg caagatgaat tgtacatagc
     1381 cggggacttc aatttgtcca gtatcccggg cgtggcattc atcgcccgct tcaacggtaa
     1441 cgaatgggtg ggggtgggcg gtggcatgga tggcccggtg cgcgggctaa aagcataccc
     1501 cgacgggctg tacgcggccg gttggttcaa caatgcgggc aatacacccg ccgagggtct
     1561 ggcccgctgg gacggggaac aatggcacag cgtacatgat ctgcccttgt tggacgacta
     1621 tccgatcaac atggtcaatg atgtggccat cataaatgat cgcatttata tcggtgggaa
     1681 tttttccggt acaccgcagg attcaatcta ttccattgca caatgggatg gcacaacttg
     1741 gaacaatttg gatcatggtt tcgtgggcgg tctcacccag atccttaaat tggaagtgca
     1801 tgattccctg ctctacatcg cgggcgcctt cgctgatacc ggccagtacg ggcatcccct
     1861 caacccggcc agtggaatca tagcctggga tggggaggat tgggtggaat tgggcagcgg
     1921 caccgatggt gccaacatcc cgtgggtaac ggacatggcc tggcacaacg atacgctgta
     1981 cgtgtgcggc gatttcaacc gcatcggcgg tgtggaggcc ggtggtctgg cacgctggga
     2041 cgggcagcag tggtgtgccc tgctgccaag cccgcccggg gcgtacggca tcagttgcct
     2101 cacctttttc aacgacaccc tgcacctggg cggtggcctc gtgtacatgg gttccgacag
     2161 tgtgaaccgt gtggccaagt ggatcggtgg gaatcatgtg gaagagtgtg aagagccggt
     2221 gggcatcccc cagcaagtgg acaacgaagt gccgggcatc accctttacc cgaacccggc
     2281 tactgtggaa ctgaacgtgc tccaccaagg gcggccgcta ccttctgggc tcctgcggat
     2341 aaccgatacg atagggcgat cggtggcaga acggcctctg accggctcag ccgtacccct
     2401 tggccccctt gctgaaggcg tttaccatgc acaggtcacc accgaacagg gccgct
//
LOCUS       DAOQOC010000383         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25339_length_2453_cov_4.292327, whole genome shotgun sequence.
ACCESSION   DAOQOC010000383 DAOQOC010000000
VERSION     DAOQOC010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25339_length_2453_cov_4.292327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PL106_07040"
     CDS             complement(<1..274)
                     /locus_tag="PL106_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32862.1"
                     /translation="MATRAFLPPLASILLLLACPAIRAQRMEDLLPRIAAEKNDSARF
                     YLAFSGLTTSETNPVDDMHNADVLLTYGQKYGDKVGQTLGLLCLGYD"
     gene            complement(381..2264)
                     /locus_tag="PL106_07045"
     CDS             complement(381..2264)
                     /locus_tag="PL106_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNA32863.1"
                     /translation="MPKDRKFYLLLIAVSVVLVAEVFSLTGHHLPMPWAPIVFAAFVL
                     GVGHEVLRKGVKALFQLRFSSINLLMVIAVVGAFYLGEYSEAAVVITLYVLGEKLEDI
                     GIERSKSALDTLVSSIPRLVVVKGVEGPVRIDQVAVGSIILVRPGEMIPLDGVVVEGR
                     SAVDEATITGEPLPQDKSVDDKVYGGTLNKLGALEVRTTRTNSDTTLTRIVQLTYQAQ
                     ANRSRTQRFIERFARVYTPCVLVVAVLLVVVPVLFLGKPFDPWFEQAITILVIACPCA
                     LVISTPVAIYSAIGNAGGRGAVVKGGLFLEALATVRAVGLDKTRTITFGRPVVEDVTA
                     LGDTTREELLSCAAGAEALSEHPLAQCIVDRARAEGFEPHRVTGFSSTMGRGITAQCT
                     VCDHDHILIGSLDHVGAQRTVRSDAQAEVDRLRAQGKTVVVVSFGQELGGVIGIADAI
                     KPDSARAVAGLQAIGVEAVMLTGDNQQAAAHVAKQVGIAAVHGGLLPEDKAACIRELQ
                     RTKGPVAFVGDGVNDAPALATADVGIAMGALGSDTAVETAGVALMNDDVRMVPWLIRL
                     ARATLARIRINTVLAVGVKFLFLLLAFLGMSHLVLAIAADVGVTLLVILTSLRLMRWD
                     GTA"
BASE COUNT          434 a          853 c          748 g          418 t
ORIGIN      
        1 agtcataccc gaggcagagc aggccgaggg tctggcccac tttgtcgccg tacttctggc
       61 cgtaggtgag cagcacatcg gcgttgtgca tgtcgtccac cgggttggtc tccgaggtgg
      121 tgagcccgct gaaggcaagg tagaagcggg cgctgtcgtt cttctcggcg gcgatcctgg
      181 ggagcaggtc ctccatgcgc tgcgcgcgaa tggccggaca ggcgagcagc agcaggatgg
      241 aggccagagg aggcaggaat gcgcgcgttg ccatggacga tggtttgatg aggcaaaact
      301 ccacctcccg atcaccccgg ccatgcgtgg aaatacgagt tctgtgcccg ctgccaggga
      361 ccccgcccgg ttcgcaagac tcacgccgtc ccgtcccaac gcatcaaccg cagactcgtc
      421 aggatcacca gcaaggtgac gcccacatcg gcggcgatgg ccagcaccag atggctcatg
      481 cccaggaagg cgagcagcag gaagaggaac ttcaccccta ccgccaatac cgtattgatc
      541 cggatgcgcg ccagtgtagc ccgcgccaga cggatgagcc agggcaccat gcgcacgtca
      601 tcgttcatga gtgccacacc ggcggtctca acggcggtgt cgctgcccaa tgcgcccatg
      661 gcgatgccca catccgccgt ggcgagcgcc ggagcgtcgt tcaccccgtc gccaacgaat
      721 gctaccgggc ctttggtgcg ctgcagctcg cggatgcatg cggccttgtc ctcgggcagc
      781 aacccgccgt gtaccgctgc gataccgacc tgcttggcca catgggcggc cgcctgctgg
      841 ttgtcgccgg tgagcatgac ggcctccacc ccgatggcct gcaacccggc cacggcccgt
      901 gcgctgtcgg gcttgatcgc gtccgcgatg ccgatgacgc cgcccagctc ctggccgaag
      961 ctcaccacca ccaccgtctt tccctgtgcc cgcaagcggt ccacctcggc ctgcgcatcg
     1021 gaccggaccg tacgctgcgc gccgacatgg tccaggctgc cgatcaggat gtgatcgtgg
     1081 tcgcataccg tgcattgtgc cgtgatgccc ctgcccatcg tgctgctgaa ccccgtgacc
     1141 cggtgcggct cgaagccctc cgcccgcgca cggtccacga tgcactgtgc cagcggatgc
     1201 tcgctgaggg cctcggcccc cgccgcgcac gacaataatt cctcgcgcgt ggtatcgccc
     1261 agggcagtga cgtcctccac caccggacgc ccgaaggtga tggtgcgggt cttgtccaag
     1321 cctaccgccc gcaccgtggc cagcgcctcc aggaacaggc cgcctttcac caccgcaccg
     1381 cgcccaccgg cattgccgat ggcgctgtaa atggctaccg gcgtgctgat caccaaggcg
     1441 cacggacagg cgatgaccag gatggtgatg gcctgttcga accacgggtc gaagggcttg
     1501 cccaggaaca gcaccggcac caccacaagc agaacggcca ccaccagcac acagggcgtg
     1561 tacacccggg cgaaacgttc gatgaatcgc tgcgtgcggc tgcggttggc ctgcgcctga
     1621 taggtcagtt ggacgatgcg cgtgagcgtg gtgtcgctgt tggtgcgggt ggtgcgcacc
     1681 tccagggccc ccagcttgtt cagcgtgccc ccgtacacct tgtcgtccac gctcttgtcc
     1741 tgcgggagcg gctccccggt gatcgtggcc tcgtccaccg cgctccgccc ctccaccacc
     1801 acaccgtcca aagggatcat ttctcctgga cgcaccagga tgatgctgcc caccgccacc
     1861 tgatcgatgc gtacgggacc ttcgacgccc ttcaccacca cgagcctggg gatgctgctc
     1921 accagcgtat ccagcgcgct cttgctgcgc tcgataccga tgtcctccaa cttttctccc
     1981 agcacataca gcgtgatcac cacggccgcc tcggaatatt cgcccaggta gaacgccccg
     2041 accaccgcga tcaccatcag gaggttgatg ctgctgaacc gcagttggaa cagggccttc
     2101 acccctttcc ggagtacctc gtggcccacc ccgagcacga aggccgcgaa gacgatcggt
     2161 gcccacggca tgggcaggtg atgacccgtc aggctgaaca cctccgccac caacaccacc
     2221 gataccgcga tgagaagcag gtagaattta cggtccttcg gcatggttct cccctgttcg
     2281 gtgttccttg gtcatggacc gcactgcaaa gatcgctgct ccggtactcc cggccacagg
     2341 cgatccgcac cgttgttact cggtcggtgg tcaagagccg gtttgagcgg agatcgatca
     2401 tggtgggtcg aagggtgcaa gagtgcgaga gcgcgaggct caaacccttg cac
//
LOCUS       DAOQOC010000384         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25350_length_2453_cov_3.163887, whole genome shotgun sequence.
ACCESSION   DAOQOC010000384 DAOQOC010000000
VERSION     DAOQOC010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25350_length_2453_cov_3.163887"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PL106_07050"
     CDS             complement(<1..470)
                     /locus_tag="PL106_07050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNA32864.1"
                     /translation="METLASPLEDLLREAGIQPPAERPPHVVFNPALPLYVRSYHAHI
                     TKRGEELLVEADERPPAAVRLADVGSVILFGNVTVSAPALHVLMERGIPVSWHKRSGW
                     FVGHTVGTGQGNVALRAAQYAAAFDPARTLRIAQALVADKIANSRTLLRRNWKQ"
     gene            complement(604..2142)
                     /locus_tag="PL106_07055"
     CDS             complement(604..2142)
                     /locus_tag="PL106_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32865.1"
                     /translation="MKNLLLITAGRTDVQLVQQGKRHELDKRSCGVLLDELDKRGSAL
                     VDAPRDKDKVAITELPEGPLAVCTPKLDAVLKATGTPTAVLILETTRDLSDDPRLAGL
                     VIEKRLRGHGVEQVQRVSYLSGRERLEDSKNPLDAVVRREVASRLDKAIREALQGLTP
                     QDKVHLATTGGLPPANELINELVRLHAVGGPMVTALEVPDSTTGEKDDQAVPEKFHPA
                     AGYRTRWHALDLIEKGNLLAAWGAVSHLKGASVHALVEGVSAGLANFRISRSERASDH
                     DGQEWTKVVYWLSRFASSLPLGGCDIDVLRHKRLAVKSALRVELALRAGDIPRAVHGT
                     VSFYEAALWDHLLERFEKKPEYLENLYPKSARDIPGPQLIRTESKEEERPFEMRVGKK
                     DGKTYYRLHESGALLLAQDYLRSKPMKNLVEAISNKEILDGRPVCVKWLRNDVAHNEP
                     NAPLMEQAKKTMEHFKVWSTQDTFLTQSLVQNVLKELGVAHPERLLEGLLQEVRKRLL
                     APSA"
     gene            complement(2139..>2453)
                     /locus_tag="PL106_07060"
     CDS             complement(2139..>2453)
                     /locus_tag="PL106_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32866.1"
                     /translation="KLGYARPLGWGSVRMQAKQLLVLDAAGEMPKLNAQADLLAWAKA
                     HYRTTPMQQEWLAIHRCKHPDAADYPRAADKNGERNIFTFHTNLRTGHSRQRRYRKPS
                     NR"
BASE COUNT          414 a          770 c          729 g          540 t
ORIGIN      
        1 tcctgtttcc agttgcggcg cagcagggtg cgcgagttgg cgatcttgtc ggccaccagc
       61 gcctgtgcga tgcgcagggt gcgcgcggga tcgaaggcgg ccgcatactg ggcggcgcgc
      121 aaagccacat tgccctggcc ggtgcccacg gtatgcccca cgaaccagcc gctgcgcttg
      181 tgccagctca cgggtatccc gcgctccatc aggacgtgca gcgcaggggc gctcaccgtt
      241 acgttgccga agaggatcac actgcctaca tcggccaggc gcaccgcggc cggcggccgc
      301 tcgtcggctt ccaccagcag ctcctcgccg cgcttggtga tgtgcgcgtg gtaggagcgc
      361 acatacagcg gcagggccgg gttgaacacc acgtgcggcg gccgctctgc agggggctga
      421 atgcccgctt cgcgaagcag gtcttccagg ggggaggcaa gggtttccat ggcggtcggt
      481 ctgcgttcgg cattccccgg ccctgcatgg gccttgcggc cctgcgccgg gggatccttt
      541 cctttttttg atccgccatg gatcaaccac ccggagtgcc cgggccgtgc ctacccctgt
      601 acgctatgcg ctcggggcaa gcaatcgctt tcttacttcc tgcagcaggc cttccaacaa
      661 ccgctccggg tgggcaacgc ccagttcctt cagcacattc tgtaccaagg attgggtaag
      721 gaaggtgtcc tgcgtggacc ataccttgaa atgctccatg gtcttcttgg cttgttccat
      781 gagaggggca ttaggctcgt tgtgggccac atcattgcga agccacttta cacagacagg
      841 ccgcccatca agtatttcct tgtttgaaat ggcttccaca aggttcttca tcggtttgct
      901 cctaaggtaa tcctgcgcca ataaaagtgc gccgctttca tgcaacctat agtaggtttt
      961 cccatccttc ttcccgaccc tcatttcgaa ggggcgctcc tcttccttgc tctcggtgcg
     1021 gatcagttgt ggccctggga tatccctagc ggacttgggg tacagattct cgaggtactc
     1081 aggctttttc tcgaaccgct caagtaggtg atcccagaga gcagcctcgt agaaggaaac
     1141 tgtgccgtgc acggcccgcg gaatgtcacc tgcccgcaat gccaattcca cgcgtaaggc
     1201 ggatttcacg gcaaggcgct tatggcggag cacgtctata tcgcaacctc ccaaaggcag
     1261 cgatgaggca aaacgcgata accaataaac gactttggtc cattcttgcc catcgtgatc
     1321 gcttgcacgt tccgagcggc ttatccggaa gttcgctaat cctgcgctaa cgccttcaac
     1381 caacgcgtga acagaggcac ctttcaaatg gcttaccgcg ccccaagcag ccagcaggtt
     1441 gcccttttcg atcaggtcca gggcatgcca gcgcgtgcgg tagcccgccg ccgggtggaa
     1501 tttctcgggg acggcttggt catccttttc accggtggtg ctgtccggta cttccagcgc
     1561 tgttaccatg ggaccaccca cggcgtgcag gcgcaccagc tcgttgatca gctcgttggc
     1621 cgggggcagc ccgccggtgg tggccagatg gaccttgtct tgcggggtta acccctgcaa
     1681 ggcttcccgg atggccttgt ccagtcggct ggccacctcg cggcgcacca cggcatcaag
     1741 tgggttcttg gagtcctcca gtcgttctct acccgataag tacgagacgc gttgcacctg
     1801 ttccaccccg tgcccgcgca accgcttttc gatcaccagc cctgctaacc ggggatcatc
     1861 cgacaggtcg cgtgttgttt ccaggatcag taccgccgta ggtgttccag tggctttgag
     1921 cacggcatcc agcttgggcg tgcacaccgc caaggggccc tcgggcaatt ctgtgatggc
     1981 aactttgtcc ttgtccctgg gggcatcaac caatgcagat cccctcttgt ccagttcgtc
     2041 caacaacacc ccgcaggatc ttttgtccaa ttcgtggcgc ttgccctgtt ggaccagttg
     2101 cacatcggtt cggccggcgg tgatcaaaag caggttcttc atcggttact gggttttcgg
     2161 taacgccgct ggcgggaatg cccggtgcgc aggttggtgt ggaaggtgaa gatgttccgc
     2221 tcgccatttt tgtccgctgc ccgcggatag tccgctgcat ccgggtgctt gcaccggtga
     2281 atggcgagcc attcttgttg catgggggtg gtccggtaat gcgccttggc ccatgcgagc
     2341 aggtccgcct gcgcgttcag cttgggcatc tccccggcag catccaacac gagcagctgc
     2401 ttggcctgca tgcgcacgct gccccagccc aaggggcggg catagccgag ctt
//
LOCUS       DAOQOC010000385         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25394_length_2451_cov_3.168614, whole genome shotgun sequence.
ACCESSION   DAOQOC010000385 DAOQOC010000000
VERSION     DAOQOC010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25394_length_2451_cov_3.168614"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PL106_07065"
     CDS             complement(<1..1022)
                     /locus_tag="PL106_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNA32867.1"
                     /translation="MQQQGWHQDHLPGIMSWSVFPVLRYLCALLFAGSVSLLNAQVDC
                     LGVNGGAALPGTPCDDGQAFTVGDIWNVDCTCIGYCGGGPFGQAMVGGACDDMDPATV
                     NDFWWPGCICHGFCVMQYGLPGASCDDGSPATNDDIVDQWGCTCTGWQNLITGQVFLD
                     VDLDGVFGGPDMPIQNRTVQAGFSWLHTTTDSNGEFEFRVGPGFYTLLAASGNYDAPA
                     QVNPPIDLSVPGSSSTGNPLAMNPSASLADLAVDLTTTPARPIYRNWITVVCTNEGNA
                     DLGVSIPDLHIACMGIEKLIPRARDLSVFTRLLGRSATGQPITAYTSHFHGPRPGCKL
                     HIVLVD"
     gene            complement(1103..1933)
                     /gene="tsf"
                     /locus_tag="PL106_07070"
     CDS             complement(1103..1933)
                     /gene="tsf"
                     /locus_tag="PL106_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNA32868.1"
                     /translation="MAITAADVNKLRQITGAGMMDCKKALTEANGDFDAAIDILRKQG
                     QKVAAKRADRDASEGVVLGLVSADGRRGVLVCTNCETDFVAKNADFVAMATRIAETAL
                     AKGADSVDALKTQAYDTNGLTIAEKLIEQTGVIGEKIEVSACHLLDGAYVFAYNHPGN
                     KVGSLVALNKPGFETAAKDVAMQVAAMGPIALDKASTPQSVIDKELEIGKELAIQEGK
                     PADMAEKIAQGRLNKFFKESTLLAQEFIKDNKLSVEQYLKSVDKELTCTGFKRHSLTV
                     "
     gene            complement(2039..>2451)
                     /gene="rpsB"
                     /locus_tag="PL106_07075"
     CDS             complement(2039..>2451)
                     /gene="rpsB"
                     /locus_tag="PL106_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNA32869.1"
                     /translation="FNHMSKPERLQVSRQREKMEKNLGSIAHLSRLPSALFIVDIVKE
                     HIAVAEARKLNIPTFAMVDTNSDPDLIDFPIPANDDATKSIELIVDTMIAAINEGIEE
                     RKSDKERGEAIDEAEEGAEEATEEAPASEEEAKA"
BASE COUNT          416 a          749 c          771 g          515 t
ORIGIN      
        1 ttatcgacca atacgatatg caatttgcaa ccaggtcgcg ggccgtgaaa atgcgacgtg
       61 tacgcagtaa ttggctgtcc ggtcgcgctt cgtcccagca atcgagtaaa gacagacaag
      121 tcgcgagcgc gaggaatcaa tttctcgatg cccatacagg cgatatgcaa gtcagggatc
      181 gacacaccca aatcggcatt tccctcgttg gtgcagacca cggtgatcca gttgcggtaa
      241 atagggcggg ccggtgtggt ggtcaggtcc actgcaaggt ccgccagcga cgcggaaggg
      301 ttcatagcca acggattgcc tgtgctggac gagccgggca cgctcaggtc gatcggcggg
      361 ttcacttggg ccggagcgtc gtagtttccg gatgccgcca agagggtata aaagccgggg
      421 ccgacgcgga actcgaactc gccgttcgag tccgtggtgg tatgcagcca agaaaaaccc
      481 gcctgtacag ttctgttctg gatcggcatg tccggcccgc cgaacacgcc atcaaggtcc
      541 acgtccaaga agacctgacc cgtgatcagg ttctgccaac cggtgcaagt gcagccccat
      601 tggtccacga tgtcatcgtt ggtcgcaggg ctcccatcat cacacgatgc gccgggcagc
      661 ccatattgca tcacacaaaa gccgtggcag atgcagccgg gccaccagaa atcgttaacg
      721 gttgcgggat ccatgtcatc gcatgctccg ccgaccatgg cttgcccaaa agggccgccg
      781 ccgcagtatc cgatacaggt gcagtccaca ttccagatgt caccgacggt gaacgcctgc
      841 ccatcatcgc acggcgtccc cggcagtgcc gccccaccat tcacaccgag gcaatccact
      901 tgggcgttaa ggagcgatac actgcccgcg aagaggagcg cgcagagata ccgcagaaca
      961 ggaaagacgc tccagctcat gatgcccggc aggtggtctt gatgccagcc ctgctgctgc
     1021 acgaagctac ccgcttacca gaaatggaaa gccccctgcc gcgcggcagg gggcttttcg
     1081 atgcttctga caggtcagcg gatcacaccg tgagcgagtg gcgtttgaag cccgtgcagg
     1141 tgagctcctt gtccacgctc ttgaggtatt gttccacgct gagcttgttg tccttgatga
     1201 actcctgggc gagcagggtg ctctccttga agaacttgtt gaggcgcccc tgggcgatct
     1261 tctcggccat gtcggcgggc ttgccctcct ggatggcgag ttccttgccg atctccagtt
     1321 ccttgtcgat cacgctttgc ggggtgctgg ccttgtccag ggcgatgggg cccatggccg
     1381 ctacctgcat ggccacgtcc ttggccgcgg tctcgaaacc gggcttgttc aaggccacca
     1441 agctgcccac cttgttgccg gggtggttgt aggcgaacac gtaggcgccg tccagcaggt
     1501 ggcaggcgct cacctcgatc ttctcgccga tcacgccggt ctgctcgatg agcttctcgg
     1561 caatggtgag gccgttggtg tcgtacgcct gggttttcag cgcgtccacg ctgtcggcac
     1621 ccttggccag tgccgtctcg gcgatgcggg tggccatggc cacgaagtcg gcgttcttgg
     1681 ccacgaagtc ggtctcgcag ttggtacaca ccagcacgcc gcgcctgccg tcggcggaca
     1741 ccagcccgag gaccacacct tcggaggcgt cgcggtcggc gcgcttggcg gccaccttct
     1801 ggccctgttt gcgcaggatg tcgatggcgg catcgaagtc gccattggcc tcggtaaggg
     1861 ccttcttgca gtccatcatg cccgcgccag tgatctggcg gagcttgttc acatcggcag
     1921 cggtgatcgc catggtgttg ctcacggtgc tattgcaggt tgggtatctt tcttgtcttt
     1981 gttcacgtcc cagggccacg ggtgctccgg aacgatggga gggcgtgcgg tacgcggatc
     2041 aggccttggc ctcctcctcg ctggcgggtg cctcttcggt ggcctcttcg gcaccttctt
     2101 ccgcctcgtc gatggcctcg ccgcgctcct tgtcgctctt gcgctcctcg atgccctcgt
     2161 tgatggcggc gatcatggtg tccacgatca gctcgatgct cttggtggca tcgtcgttgg
     2221 ccggaatggg gaagtcgatc aggtccggat cgctgttggt gtccaccatc gcgaaggtgg
     2281 ggatgttcag cttgcgggcc tcggccacgg cgatgtgctc cttcacgatg tccacgatga
     2341 acaacgcgct gggcaggcgg ctgaggtggg cgatggagcc caggttcttc tccatcttct
     2401 cgcgctggcg gctcacctgg aggcgctcgg gcttgctcat gtggttgaag g
//
LOCUS       DAOQOC010000386         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25522_length_2445_cov_3.346862, whole genome shotgun sequence.
ACCESSION   DAOQOC010000386 DAOQOC010000000
VERSION     DAOQOC010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25522_length_2445_cov_3.346862"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PL106_07080"
     CDS             complement(<1..343)
                     /locus_tag="PL106_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNA32870.1"
                     /translation="MKVLFIDHVHPVLSERLTIAGHTCEDVSELEGPALFAQLHDATG
                     IVVRGRVPLDAATLGHAPRLRFIARAGAGMENIDRAECERRGIRLYNSPEGNRDAVGE
                     HEAFEQGVAGQP"
     gene            complement(340..1731)
                     /gene="mgtE"
                     /locus_tag="PL106_07085"
     CDS             complement(340..1731)
                     /gene="mgtE"
                     /locus_tag="PL106_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNA32871.1"
                     /translation="MSFNLSQQHLERIQELVAQKNEAAVLHEVSELHPADIAEVMDRL
                     DLEEARYVHGLLEPGTAADVITELPEDRRDALLGTLSAEEIAGEVIEHLDTDDAADVI
                     AELPDDVREEVLAKIDDSELKSEITELLTYGENTAGSLMAKELVKVNVGASMLDCVRV
                     LRQQADLDAVYRIHVVDDHDRFVGSIPLKQLITESLRSPVKKVYDPDAVAVSANTDVE
                     EVARLMEKYNLADVPVVDERQRLVGRITFDDVMDSVRKEETEDAHKMGGMEALEYSYS
                     SSSLLEMVRKRAGWLIVLFIGEGFTATAMGFFEGQIAKAVVLALFVPLIISSGGNTGS
                     QASSLIIRALAVGDITVNEWWRIFRREMSVGMILGTALGVIGFARVAIWSQFVNVYGP
                     HWPRIGIAVGVSLVGVVLWGNLIGSLFPLLLKRLGLDPAVSSAPFVATVVDVTGLVIY
                     FTIASLFLAGILL"
     gene            complement(1791..>2445)
                     /gene="rsmA"
                     /locus_tag="PL106_07090"
     CDS             complement(1791..>2445)
                     /gene="rsmA"
                     /locus_tag="PL106_07090"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNA32872.1"
                     /translation="IEVGPGTGALTRHLIARGDDLTCFEVDRESVAHLATAHPDLRVL
                     QDDFLRADLSRIAKGALAVIGNFPYNISTQIVFKVLEHRDRVTEVVGMFQKEVADRIC
                     AGPGSRTYGITSVLAQAWYTCEPLFIVEPGSFIPPPKVRSAVIRMRRNAIEKLDCDER
                     LLFQVVKAAFNQRRKTLGNALKAFAPLRSDVPERFTKLRAEQLSVADFISLASACTA"
BASE COUNT          417 a          822 c          767 g          439 t
ORIGIN      
        1 ccggctggcc agcaactccc tgctcgaagg cctcgtgttc gcccaccgca tcgcggttgc
       61 cttccggtga gttgtagagg cggatccccc ggcgctcgca ctcggcccga tcgatgttct
      121 ccatgccggc tcccgcacgc gcgatgaacc gcagtcgcgg tgcgtgcccc agtgttgcgg
      181 catcgagcgg cacccggccc cgaacgacga tcccggtggc atcgtggagc tgcgcgaaca
      241 gggccggacc ttcgagttcg ctcacatcct cgcacgtgtg ccccgcgatc gtgagccgtt
      301 cgctcaatac cggatgcacg tgatcgatga acaggacctt catagcagga tccccgccag
      361 gaagagcgaa gcgatggtga agtagatgac caggccggtg acatcgacca cggtggccac
      421 gaagggagcg ctgctgacgg ccggatcgag gcccagccgt ttgagcagca gcgggaacaa
      481 ggagccgata aggttgcccc acagcaccac gccgacgagc gacaccccga ccgcgatgcc
      541 gatccgtggc cagtgcgggc cgtaaacgtt cacgaactgg ctccagatgg ccacgcgcgc
      601 gaagccgatc acgcccaggg ccgtaccgag gatcatcccc acgctcatct cccggcggaa
      661 gatgcgccac cattcgttca cggtgatgtc ccccacggcg agcgcgcgga tgatgagcga
      721 cgatgcctgg gatccggtat tcccgccgct gctgatgatc agcggcacga acaacgcgag
      781 caccacggcc ttggcgatct gtccctcgaa gaagcccatg gccgttgcgg tgaatccctc
      841 gccgatgaaa aggacgatga gccagccggc ccgtttcctc accatctcca gcaggctgct
      901 gctgctgtag ctgtactcca gggcctccat accgcccatc ttgtgcgcgt cctcggtctc
      961 ctccttgcgc accgaatcca tcacatcgtc gaaggtgatg cgccccacca gccgctggcg
     1021 ctcgtccacc acgggcacat cggccaggtt gtacttctcc atcaggcggg ccacctcctc
     1081 cacatcggtg ttggcgctca cggccacggc atcggggtcg tacaccttct tcacgggtga
     1141 gcgcaggctc tcggtgatca actgcttcag cgggatcgag cccacgaacc ggtcatgatc
     1201 gtcgacgacg tggatgcggt agacggcgtc caggtcggcc tgttggcgaa gcacgcgcac
     1261 gcaatcgagc atgctcgcgc ccacgttcac cttcacgagt tccttggcca tcaggctgcc
     1321 ggccgtgttc tcgccgtagg tgagcagctc ggtgatctcg ctcttcagtt cgctgtcgtc
     1381 gatcttggcg agcacctcct cgcgcacatc gtccggcagt tcggcgatca cgtcggccgc
     1441 atcgtcggtg tcgaggtgct cgatcacctc cccggcgatc tcttcggcgc tcagggtgcc
     1501 cagcagcgcg tcgcggcgat cctccggcag ctcggtgatc acgtcggccg cggtgccggg
     1561 ttccagcagg ccatgcacgt accgcgcctc ttccagatcc agccggtcca tcacctcggc
     1621 gatgtccgcc gggtgcagct cgctcacctc atggagaacg gcagcctcgt tcttctgggc
     1681 gacgagttcc tggatgcgtt ccaggtgctg ctggctgagg ttgaagctca tcgcggtacc
     1741 gtccgttggc gtgccgcgcg aaactatggt gaagctccct gaggacgggc tcaggcggta
     1801 caggcgctgg ccagcgaaat gaagtctgct acggaaagct gttcggcgcg cagtttcgtg
     1861 aagcgttccg gcacatcgga cctcagcggg gcgaaggcct tcagggcgtt gcccagggtc
     1921 ttccgccgct ggttgaaggc cgccttcacc acctggaaga ggaggcgctc gtcgcaatcg
     1981 agcttttcga tggcgttccg ccgcatacgg atcacggcgc tgcgcacctt gggcggggga
     2041 atgaatgatc ccggttccac gataaagagc ggctcgcagg tgtaccaggc ctgggccagt
     2101 acgctcgtga tgccgtaggt cctgctgcca ggaccggcgc agatgcggtc ggccacctct
     2161 ttctggaaca tgcccaccac ctcggtcaca cggtcgcgat gttccagcac cttgaacacg
     2221 atctgcgtac tgatgttgta cgggaagttg ccgatcaccg ccagggcacc cttggcgatg
     2281 cgcgacaggt cggcccggag aaaatcatcc tgaagaacgc ggagatccgg atgggcggtg
     2341 gccaggtgcg caacgctttc gcggtccacc tcgaagcagg tgaggtcatc gccgcgcgcg
     2401 atcaggtgcc tggtgagcgc acccgtaccg ggccccactt cgatc
//
LOCUS       DAOQOC010000387         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25553_length_2443_cov_3.629816, whole genome shotgun sequence.
ACCESSION   DAOQOC010000387 DAOQOC010000000
VERSION     DAOQOC010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25553_length_2443_cov_3.629816"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..2075)
                     /locus_tag="PL106_07095"
     CDS             complement(153..2075)
                     /locus_tag="PL106_07095"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNA32873.1"
                     /translation="MRKAALLLVLSPLFASAQIGYDHAIFEEVPCAHRRDPGPLPRSD
                     AEPTRGMDLRYVRAAWEVDPNVRAITGTVMLRFVSTHPLSELVLDLSDTLIVDEVLYH
                     GSQNTFTLAPGDLLTIPLGQELPAGATDSVEVSYHGIPRSTGFGSFATGQQPDSSAAL
                     WTLSEPYGAKEWWPCKDDLNDKIDSIDLYITTPEGYRAGSNGLLVSEVMGNGHVTYHW
                     RHRYPIATYLISMAVADYLVFTHDIVIGTDTIPMLTYSWHDDPGMAELNGYDAGEQMT
                     LYSELFGLYPFAEEKYGHAQFGWGGGMEHQTMTSMGGWSYELSAHELAHQWFGDKVTC
                     GSWQDIWLNEGFATYLQGMCYEFLAPEYWYGYKAGKIESIISEPGGSVFRADTSSTES
                     IFSGRLTYNKGAMVLHMLRWVCGDSAWFQGVRDYLEDPGLAYGSARTADLQAHLEATS
                     GLDLDGFMADWFTGEGYPTYTLPWSQASDGAVSLTLYQSSSHPSVDFFEMPVPVRFKN
                     ADTDTLVVLDHTVSGQTFTFILPFMADSALLDPDLRIVSGQNIVTRVPEQQMNTAGYV
                     LAPNPVSDRLIVRPLSSRGQVRLTIHDDLGRLRWSRMDQLNGDLTVDVRSLTAGCYVL
                     SVDDGVLVSRARFVKE"
     gene            complement(2097..>2443)
                     /locus_tag="PL106_07100"
     CDS             complement(2097..>2443)
                     /locus_tag="PL106_07100"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNA32874.1"
                     /translation="AEYLVTQMFFDNTKYLGFVERCRNEGIKVPIIPGLKPLTTLKQI
                     AFIPKTFAIDLPDGLAKELVACRSDADVKAVGTAWCIQQARELKAAGVPCLHFYTMGR
                     NDAVRNILSQVF"
BASE COUNT          476 a          751 c          753 g          463 t
ORIGIN      
        1 acaatagcga ctggcgcgga ctactgctct acgaccgtga tgcgcaggag gaagtggagg
       61 gatggctgcg acaggacccc ttcgtaaaga cgggacggct cgcgtacacg atccatccgc
      121 ggtacggtgc catcggtacc acgttgaagt gatcactcct tcacgaaacg tgcgcgcgaa
      181 acaagcacac catcatccac cgacagcacg tagcagccag cggtgagtga tctgacatcc
      241 acggtgagat ccccgttcag ttggtccatg cgcgaccacc gcaagcggcc caggtcatca
      301 tggatggtca atctgacctg cccccgggag gacagcggtc gaacaatgag cctgtcgctc
      361 accggattcg gcgcaagcac gtacccggcc gtattcattt gctgctccgg tacccgggtc
      421 acgatgttct gtccgctcac gatccgcagg tcaggatcca ataacgcact gtccgccatg
      481 aacggaagaa tgaacgtgaa ggtctgaccg ctcaccgtgt gatccagcac gacgagcgta
      541 tccgtatccg cgttcttgaa ccggaccgga acgggcatct cgaaaaagtc caccgaggga
      601 tgggagctgc tctggtacag cgtgagcgat accgctccat cagaagcttg tgaccaggga
      661 agcgtgtagg tgggatagcc ctcgccggtg aaccagtccg ccatgaaccc gtcgaggtcc
      721 aggccactgg tcgcttccag atgcgcctgc aggtcagcgg tgcgtgcgct gccgtacgcg
      781 agtcccgggt cctccagata gtcccgaaca ccctggaacc acgcgctatc accacatacc
      841 cagcgcaaca tgtgcaggac catggcgccc ttgttgtacg tgagccgacc actgaatatg
      901 ctttcggtgc tgctggtgtc cgcccggaag acgcttccac ccggctccga aatgatactc
      961 tcgatcttgc ccgccttgta cccgtaccag tattcagggg ccaggaattc atagcacatg
     1021 ccctgcaggt aggtggcaaa gccctcgttc aaccagatgt cctgccagct gccgcaggtc
     1081 accttgtcgc cgaaccactg gtgggccagt tcgtgcgcac tcaattcgta gctccagccc
     1141 cccatgctcg tcatggtctg gtgttccatg ccaccgcccc aaccgaactg tgcgtggccg
     1201 tatttctcct cggcgaaagg atacaggccg aacagctcgc tgtacagggt catctgttct
     1261 ccagcatcgt acccattcag ttccgccatg ccggggtcgt cgtgccaaga gtacgtgagc
     1321 atgggaatgg tatccgtgcc gatgacgatg tcgtgcgtga atacgaggta gtcggccacc
     1381 gccatggaga tcaggtaggt ggcgatggga taacggtgcc gccagtggta ggtcacatgc
     1441 ccattcccca tgacctcgga tacgagcagg ccgttcgagc ccgcgcggta gccttccggg
     1501 gttgtgatgt acaggtcgat ggaatcgatc ttgtcgttga ggtcatcctt gcagggccac
     1561 cactccttgg cgccgtacgg ttcgctcaag gtccagagcg cggcgctgct gtcgggttgc
     1621 tggcccgtgg cgaagctgcc gaagcccgtg gatcgcggga tgccatggta gctcacctcg
     1681 accgaatcgg tcgcgcccgc cggaagctcc tgccccaacg gtatcgtaag caggtcgccg
     1741 ggcgcgaggg tgaaggtgtt ctgcgaaccg tggtacagca cttcgtcaac gatgagcgta
     1801 tcactcaggt cgagcaccag ttcggagagc ggatgcgtgg aaacgaagcg cagcatgacc
     1861 gtgccggtga tggcgcgcac gttgggatcc acctcccagg cggcccgcac gtagcggagg
     1921 tccatgccgc gcgtcggttc ggcgtcgctg cgcggcaggg gccccggatc gcgccggtgg
     1981 gcacagggta cctcttcaaa aatggcgtga tcgtatccga tctgggcgct ggcgaacagc
     2041 ggtgacagga ccagcagtag tgcggccttc ctcatgaggc caagatagcg cggggatcag
     2101 aacacctgcg acaggatgtt gcgcaccgcg tcgttcctgc ccatggtata gaagtgcagg
     2161 cagggcaccc cggcagcttt caattcccgg gcctgctgga tgcaccaggc tgtgcccacg
     2221 gctttcacgt cggcatcgct ccggcacgcc accagttcct tcgccaagcc gtcgggcaga
     2281 tcgatggcga aggtcttcgg gatgaaagcg atctgtttga gggtggtgag cggcttgagg
     2341 cccgggatga tgggcacctt gatcccctcg ttccggcagc gctccacgaa gcccagatac
     2401 ttcgtgttgt cgaagaacat ctgcgtcacg aggtattcgg cac
//
LOCUS       DAOQOC010000388         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25587_length_2441_cov_3.765298, whole genome shotgun sequence.
ACCESSION   DAOQOC010000388 DAOQOC010000000
VERSION     DAOQOC010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25587_length_2441_cov_3.765298"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PL106_07105"
     CDS             <1..1024
                     /locus_tag="PL106_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA32875.1"
                     /translation="HIRSVLAYSIYFPVVEVLGAISLAFLIWWGMGDVLEGTATFGEL
                     VAYIQFINMLFRPIRQLADRFNTLQMGMVGSERIFKVLDTEESIADTGTRSTSALRGD
                     IAFENVTFGYDPAVPVLHGISFSAKAGEMIALVGATGSGKSSIINVLGRAYEYQDGRV
                     LLDGHDIREYKLDELRTSVATVLQDVFLFSDTVLNNITLNDPSITREHVIEAAKAVGA
                     HAFIEALPQGYDTDVRERGSVLSTGQRQLLAFIRAYVNRPKVLVLDEATSSVDSLSEQ
                     LITEATARITKGRTSIVIAHRLSTVQDADRILVVDKGRIIEQGSHQELLAHGGEYKKL
                     YDLQFH"
     gene            1100..>2441
                     /locus_tag="PL106_07110"
     CDS             1100..>2441
                     /locus_tag="PL106_07110"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HNA32876.1"
                     /translation="MRPDLRTTLLILILALVQSAQGQQLIPTRGKDFWMGFMRNYDGP
                     QHQDLFISSDVSTSGTVNAPLFGWSTTFTVAANTVTTVQVPVGLMNNASETVDTRGIR
                     IQSEDTVAVYALSFQSATADATVVYPVKSCGTEYRVQAYRGFPGIAQYSSEFLIVATA
                     DGTEVEITTTAATVGGHAAGDTWTVALNTGESYQVQALEDVGDLTGSLVRGTPESGGC
                     RPFALFGGSVCPQVPAGCAACDHLYEQNLPAPAWGASYYSVPWEATYAYTYRILAHED
                     NTNVTVDNGTPITLNAGQYAEVNGTDGAHCFNGDRPFSVAQFMQGSDCAGDSDPAMLL
                     LNAEAQKIDDITFATVVSQAITAHFINVVIDAGDVGTVVLDGSTVPAIEFTPFPACSD
                     HLYATLPLTPGSHSISCPNGLTGYVYGTGPNYETYAYSVGAFTSLPQLAYDTVYC"
BASE COUNT          486 a          797 c          768 g          390 t
ORIGIN      
        1 gcatatccgg agcgtactgg cgtacagcat ctacttcccg gtggtggaag ttctgggtgc
       61 catatcgctg gccttcctga tctggtgggg catgggcgac gtgctggagg gcacggccac
      121 cttcggcgaa ctggtggcgt acatccaatt catcaacatg ctgttccggc ccatacggca
      181 gttggccgac cggttcaaca cgctgcaaat gggcatggtg ggcagtgagc gcatcttcaa
      241 ggtgctcgat accgaggagt ccatcgccga caccggcacc cgttccacga gcgccttgcg
      301 cggcgacatc gcattcgaga acgtgacctt cggctacgac cccgcggtgc ccgtgctgca
      361 cggcatcagc ttctccgcga aggccggcga gatgatcgcg ctggtgggcg ccacgggcag
      421 cgggaagagc agcatcatca acgtactggg ccgggcgtac gaataccagg acggacgtgt
      481 gctgctcgat gggcacgaca tccgggaata caagctggat gaactgcgca ccagcgtggc
      541 cacggtgttg caggatgtct tcctgttcag cgacacggtg ctcaacaaca tcaccttgaa
      601 cgacccgtcc atcacgcgcg aacacgtgat cgaagcggcg aaggccgtgg gcgcacatgc
      661 cttcatcgaa gcgttgccgc aagggtatga tacggatgtg cgggaacggg gcagtgtatt
      721 gagcaccggt caacggcaac tgctcgcctt catccgggcc tacgtgaacc ggcccaaagt
      781 gctcgtgctg gacgaagcca ccagcagcgt ggacagcctg agcgagcagt tgatcaccga
      841 ggccacggcg cgcatcacca aggggcgcac cagcatcgtc atcgcccacc gcctgagcac
      901 ggtgcaggac gccgaccgca tcctggtggt ggacaagggg cggatcatcg agcagggaag
      961 ccaccaggaa ttgctggccc acggcgggga atacaagaag ctgtacgacc tgcagttcca
     1021 ttgatccagg ccccgtacaa cctccgcatc gcgggagaag tctacattga ggccgaaaac
     1081 acgcaccacc tccgtgccca tgcgccccga cctgaggacc accctgctca tactcatcct
     1141 tgcgctcgta caaagcgccc aaggccagca gctcatcccc acccggggca aggacttctg
     1201 gatgggcttc atgcgcaact acgacggccc gcaacaccag gacctcttca tcagcagcga
     1261 tgtgagcacc agcggaacgg tgaacgcgcc cttgttcggc tggagcacca ccttcacggt
     1321 ggcggccaac acggtcacca ccgtgcaggt gcccgtcggg ctgatgaata acgcaagtga
     1381 aacggtggac acgcggggga tccgcatcca gagcgaggac acggtggccg tgtacgcgct
     1441 cagtttccaa agcgcaacgg ccgatgccac cgtggtgtat ccggtgaagt cgtgcggtac
     1501 cgaataccgg gtacaggcct accggggctt cccgggcatc gcgcagtaca gttcggagtt
     1561 cctcatcgtg gccacggccg acggtaccga ggtcgagatc accaccacgg cggccaccgt
     1621 gggcgggcat gcggccgggg acacctggac cgtggcgctc aacaccggcg agagctacca
     1681 ggtgcaggcc ttggaggacg tgggcgacct cacgggcagc ctggtgcgcg gaacacccga
     1741 gagtggtggc tgcaggccct tcgccctgtt cgggggcagc gtgtgcccgc aggtgcctgc
     1801 cggatgcgcg gcgtgcgatc acctgtacga gcagaacctg cccgctcccg catggggcgc
     1861 cagctattac agcgtgccct gggaggccac gtacgcgtac acctaccgca tactggcgca
     1921 cgaggataat acgaacgtca ccgtggacaa cgggaccccg atcaccttaa acgccgggca
     1981 gtacgccgag gtgaacggca ccgacggggc gcactgcttc aacggcgacc ggcccttcag
     2041 cgtggcgcag ttcatgcagg gcagcgactg tgcgggcgac agcgaccccg ccatgctgct
     2101 gctgaacgcc gaggcgcaga agatcgacga catcaccttc gccaccgtgg tcagccaggc
     2161 catcaccgcg cacttcatca acgtggtgat cgatgccggc gatgtgggca cggtggtgct
     2221 cgatggcagt acggtgcccg ctatcgaatt cacacccttc ccggcctgct cggaccattt
     2281 gtacgccacc ctgccgctca cccccggcag ccacagcatc agctgcccga atggacttac
     2341 cggatacgtg tacggtaccg ggcccaacta tgagacctac gcctactcgg tgggcgcctt
     2401 cacctcgctc ccccaactgg cctacgacac ggtgtattgc g
//
LOCUS       DAOQOC010000389         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25606_length_2440_cov_3.689308, whole genome shotgun sequence.
ACCESSION   DAOQOC010000389 DAOQOC010000000
VERSION     DAOQOC010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25606_length_2440_cov_3.689308"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="PL106_07115"
     CDS             complement(<1..1744)
                     /locus_tag="PL106_07115"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNA32877.1"
                     /translation="MNIGIIGAGPVGMILAAKLQEAGCNVALCERNEVKRNKIMDEGL
                     VLHGTLNSTTRFAKIYASIAELAELDLDYLVFSIKTYSVADALAEAAHLNSPKLMVVA
                     AQNGIDVEEALVPAFGESKILRMVVNYAGNLTAPNTVKVTFFIPPNYIGSINDSHPEK
                     AKELAAVLNQVELHTEAVDSFDLLRRVWHKTILNSSLSALCGVGRLTMAEAVNDSDTA
                     ELVEQTIRECVEVAEKEKIVFPDDFVRQCLRYLKKGGDHFPSLAVDLINGRRTEIDHF
                     NAKVVEYGRKHYVRTSLNLSFTNMVKAMSNRNIVYRVPGSTGDVVKRIMDKALADPKA
                     VPGQYKKKNCFLGIDLGSSYSKFCVIDDQGTALFRYFLPTMSNDKQAFKNVMQAINSN
                     FNVQKSCATGYGRKHFIDSDIARTEINCAAAGVSFSLKGEKNIIDIGGEDIKVIRCDK
                     DDTVQNFYMNDKCASGTGAFLSEIAERAGINIGEMSAMAALSTYSKELNSFCTVFAKT
                     EIMNWIFEGMSREDISRGIYISICNRMGKVRLDPGVPTVLIGGVIAHHPYLKELLSEK
                     LKRDMVIAAHPQFTV"
     gene            complement(1741..>2440)
                     /locus_tag="PL106_07120"
     CDS             complement(1741..>2440)
                     /locus_tag="PL106_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNA32878.1"
                     /translation="FKEVVKLSKEATLLWQRVLKTAASDTPPISFFDGTIHMGPIVVL
                     RGTEVAKAYYAQLLQELEGKIAQGQGFLPKVRSRIFWEGMPIWGKIRMLSDLFCENGA
                     AVVASTYCSSWVFDQFDEADPWTSTARAYTGIFINRGEGAKMKMLAEWFGEYGIDGIV
                     YHDSKTCFNNSNAKMGMPERLKAITGVPSLIIEGDLCDLRFFSEGQSITKIETFLEQL
                     AESPAVHGRKRTVQ"
BASE COUNT          384 a          748 c          797 g          511 t
ORIGIN      
        1 acacggtgaa ctgcgggtgc gcggcgatca ccatgtcgcg cttgagcttc tcgctgagca
       61 gttccttcag gtaggggtgg tgcgcgatca ccccgccgat gagcaccgtg ggcacgccgg
      121 gatcgagccg caccttgccc atgcggttgc agatggagat gtagatgcca cgggagatgt
      181 cctcgcgcga catgccctcg aagatccagt tcatgatctc cgttttggca aacacggtgc
      241 agaagctgtt gagctccttg ctgtaggtgg agagcgcggc catggcgctc atttcgccga
      301 tgttgatgcc ggcgcgctcg gcgatctccg agaggaaggc gccggtgccc gaggcgcact
      361 tgtcgttcat gtagaagttc tgcaccgtgt cgtccttgtc gcagcggatc accttgatgt
      421 cctcaccgcc gatgtcgatg atgttcttct cgcccttgag gctgaaggag acgcccgcgg
      481 ccgcgcagtt gatctccgtg cgggcgatgt ccgaatcgat gaagtgcttg cggccgtagc
      541 ccgtggcgca gctcttctgc acgttgaagt tgctgttgat cgcctgcatc acgttcttga
      601 aggcctgctt gtcgttgctc atcgtgggca ggaaataccg gaagagcgcg gtgccctggt
      661 cgtcgatcac gcagaacttc gagtacgacg agcccaggtc gatgcccagg aaacagttct
      721 tcttcttgta ctgcccgggc acggccttgg ggtcggccag ggccttgtcc atgatgcgct
      781 tcaccacgtc gccggtgctg ccgggcacgc ggtacacgat gttgcggttg ctcatggcct
      841 tcaccatgtt ggtgaaggag aggttgagcg aggtgcgcac gtagtgcttg cgcccgtact
      901 ccacgacctt ggcgttgaag tggtcgatct ccgtgcgcct gccgttgatc aggtccacgg
      961 ccagcgaggg gaagtggtcg ccgcccttct tgaggtagcg caggcactgg cgcacgaaat
     1021 catcggggaa gacgatcttc tccttctcgg ccacctccac gcactcgcgg atggtctgct
     1081 ccaccagctc ggcggtgtcg ctgtcgttca ccgcctcggc catggtgagg cgccccacgc
     1141 cgcacagcgc gctgagcgag gagttgagga tggtcttgtg ccacacgcgc cgcagcaggt
     1201 cgaaggagtc caccgcctcg gtgtgcagct ccacctggtt gagcaccgcc gccagctcct
     1261 tggccttctc ggggtggctg tcgttgatgg agccgatgta gttgggcggg atgaagaagg
     1321 tgaccttcac cgtgttgggc gccgtgaggt tgccggcgta gttcaccacc atgcgcagga
     1381 tcttcgactc gccgaaggcg ggcaccagcg cctcctccac gtcgatgccg ttctgggcgg
     1441 ccaccaccat cagcttgggc gagttgaggt gcgcggcctc cgccagcgca tccgccacgg
     1501 aataggtctt gatggagaag accaggtagt ccaggtccag ctcggccagc tcggcgatgc
     1561 tggcgtagat cttcgcgaag cgcgtggtgg agttcagggt gccgtgcagc accaggcctt
     1621 cgtccatgat cttgttgcgc ttcacctcgt tgcgctcgca cagggccacg ttgcagccgg
     1681 cctcctgcag tttggccgcc aggatcatgc ccacagggcc cgcgccgatg atgccgatgt
     1741 tcattgcacc gttcgttttc tgccgtgtac cgccggactt tccgcgagct gctccaggaa
     1801 ggtctcgatc ttggtgatgc tttgcccctc gctgaagaag cgcaggtcgc acaggtcgcc
     1861 ctcgatgatc agtgatggca cgccggtgat ggccttgagc cgctcgggca tgcccatctt
     1921 ggcgttggag ttgttgaagc aggtcttgct gtcgtggtac acgatgccgt cgatgccgta
     1981 ctcgccgaac cactcggcga gcatcttcat cttggcgccc tcgccgcggt tgatgaagat
     2041 gccggtatag gcccgcgcgg tggaggtcca ggggtcggcc tcatcgaact ggtcgaagac
     2101 ccagctgctg caataggtgg aggccaccac ggcggcgccg ttctcgcaga acaggtcgct
     2161 gagcatgcgg atcttgcccc agatgggcat gccctcccag aagatgcgcg aacgcacctt
     2221 ggggaggaag ccctggcctt gggcgatctt gccttcgagc tcctgcagca gctgcgcgta
     2281 gtacgccttg gccacctcgg tgccgcgcag caccacgatg ggccccatgt ggatggtgcc
     2341 gtcgaagaag ctgatcggcg gggtgtccga ggccgccgtc ttcagcacgc gctgccagag
     2401 cagcgtggcc tccttgctca gtttcaccac ttccttgaag
//
LOCUS       DAOQOC010000390         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25702_length_2436_cov_2.620748, whole genome shotgun sequence.
ACCESSION   DAOQOC010000390 DAOQOC010000000
VERSION     DAOQOC010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25702_length_2436_cov_2.620748"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="secA"
                     /locus_tag="PL106_07125"
     CDS             complement(<1..743)
                     /gene="secA"
                     /locus_tag="PL106_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479853.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNA32879.1"
                     /translation="MPMFGSIVKSIFGDKAKRDLKEVQPLVEQVKAEYAKLANLSHNE
                     LRAKTADLKQRIAEGIKEEQGKVDALRAEIDADPGMDIHARETRYQEIDKLEEKILAR
                     IEEVLLVILPEAFAIVKETARRFKENSSIRVRATDLDRELAAKRDSIRIEGDEAVWSN
                     TWIAAGNPVTWDMVHYDVQLIGGIALHKGKIAEMSTGEGKTLVSTLPVFLNALSGRGV
                     HLVTVNDYLAKRDAEWMGPLHEFHGLRVD"
     gene            complement(950..1171)
                     /locus_tag="PL106_07130"
     CDS             complement(950..1171)
                     /locus_tag="PL106_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNA32880.1"
                     /translation="MYWTLELASYLEDAPWPATKDELIDFAMRTGAPLEVVENLQAIE
                     DDEEIFESIEEIWPDYPSKEDFFFNEDEY"
     gene            complement(1295..1846)
                     /locus_tag="PL106_07135"
     CDS             complement(1295..1846)
                     /locus_tag="PL106_07135"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNA32881.1"
                     /translation="MKIYTRTGDKGQTGLLGGKRVPKDHARIEAYGTVDELNSHIGLL
                     RDLAAGLQQELLLDIQEKLFSLGSRLASASDEEADRFKVPQVGDADITALETAMDDMN
                     TVLPEMRHFILPGGHPAVSQAHICRTVCRRAERAVVTLGSQEAVPEHIIRYLNRLSDL
                     FFMLARWLGHTLGSPEIPWKPRG"
     gene            complement(1894..>2436)
                     /locus_tag="PL106_07140"
     CDS             complement(1894..>2436)
                     /locus_tag="PL106_07140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA32882.1"
                     /translation="SDMARTSLKPARQAQMLFRLAEYFMPETILELGTSFGLSTLYLA
                     RGAGGAQVTTIEGCPHTHRIAQHHFERLRQNNISALLGSFRSSLPFVLRTHGSLDMVF
                     IDGHHAHDPTLEYFEACLQKAHNNSVFILDDIHWSKGMEAAWEAVKQHPRVTVTIDLY
                     DLGLVFFRQEQAPQHFRLRY"
BASE COUNT          414 a          737 c          763 g          522 t
ORIGIN      
        1 cagtccacgc gcaacccatg gaactcgtgc agcgggccca tccactcggc gtcgcgcttg
       61 gccaggtagt cgttcactgt gaccaggtga accccacgac ccgacagggc attgaggaac
      121 acgggcaggg tactcaccag cgttttgcct tcgccggtgc tcatctcggc gatcttcccc
      181 ttgtgcagcg cgataccacc gatcagctgc acgtcgtagt gcaccatgtc ccaggtaacg
      241 gggtttcccg ccgcgatcca ggtattgctc cagacggcct cgtccccttc gatccgtatg
      301 ctgtcacgtt tggcggcgag ttcgcggtcc aggtcggtgg cacgcacacg gatggagctg
      361 ttctccttga agcgacgcgc ggtctccttc acgatggcga aggcttcggg caggatcacc
      421 agcagcactt cctcgatccg cgccaggatc ttctcctcca gtttgtcgat ctcctggtag
      481 cgcgtttcgc gggcgtggat gtccatgccg gggtcggcat cgatctcagc gcggagcgca
      541 tccaccttgc cctgctcttc tttgatgccc tcggcgatgc gctgcttgag gtcggcggtc
      601 ttcgcgcgca actcgttgtg gctgaggttg gcgagcttgg cgtactccgc cttcacctgc
      661 tcaaccaggg gctgtacctc tttgaggtcg cgcttggcct tgtctccgaa aatcgacttg
      721 acgatggaac cgaacatcgg gatgcgtgta ctatggtgcg ttcgtcgggg ccaacggtcc
      781 cgcgctccgt tcttgaaaag gtccgcaaag gtagccggat aaggtcccgg ccacctcggt
      841 ccaaaagcat ggatcgggcc atgtgcgcac cagcggtcag gctggcagga aaaggaagag
      901 gacccctcca tctttcgacg gaagggtcct caagggatcc tggtggaact cagtactcgt
      961 cctcgttgaa gaagaagtcc tctttgctgg ggtagtcggg ccagatctcc tcgatgctct
     1021 cgaagatctc ctcatcgtcc tcgatggcct ggaggttctc caccacttcg agcggcgcgc
     1081 ctgtgcgcat ggcgaagtcg atcagctcgt ctttcgtggc gggccaaggg gcgtcctcga
     1141 ggtacgaggc cagttcaagc gtccagtaca tggtgcgtgt ggccgttttt tttcagggcc
     1201 gcaaatatag acgggggccg atcccgccga acgatcgatt ttcaacgggt gttgacgcga
     1261 actttcctca gcgttcaccg gggtttccgg cgattcagcc acgcggcttc caagggatct
     1321 cgggggatcc gagggtgtgg ccgagccacc gggccagcat gaagaacagg tcgctcagcc
     1381 ggttgagata gcggatgatg tgttccggca cggcttcctg cgagcccaat gtgaccacgg
     1441 cccgttctgc gcggcggcat acggttcggc agatgtgtgc ttgcgaaacg gcgggatggc
     1501 caccgggaag gatgaaatgg cgcatctcgg gcagcacggt gttcatgtcg tccatcgcgg
     1561 tttccagcgc cgtgatgtcg gcatcgccca cttgtggcac cttgaacctg tccgcctcct
     1621 catcgctggc actggccaat cgcgatccca ggctgaagag cttctcctgg atatcgagca
     1681 acaattcctg ttggaggccc gctgccaggt cgcgcaacag accgatgtgg ctgttcaact
     1741 catccacggt gccgtacgcc tcgatgcgcg catgatcctt cggcacgcgc ttcccgccca
     1801 gcaggccggt ctggccttta tcgccggtgc gggtgtagat cttcatacgt gaggagaatg
     1861 ccgtgttcgg ggtgaacgcg tgcggggtgc tgctcagtac ctcagcctga agtgctgcgg
     1921 cgcttgctcc tgccggaaaa aaacgagtcc gaggtcgtac aggtcgatgg tgacggtgac
     1981 acgcgggtgc tgcttcacgg cctcccacgc ggcttccatc cccttgctcc agtggatatc
     2041 gtccaggatg aacacactgt tgttgtgcgc tttttgcagg caggcttcga agtattcgag
     2101 cgtgggatcg tgcgcatgat gtccgtcgat gaagaccatg tccagcgatc cgtgcgtgcg
     2161 caacacgaag ggcagactgc tgcggaaact gcccagcaag gcactgatgt tgttctgccg
     2221 cagccgttcg aaatgatgct gcgcgatgcg atgcgtatgc gggcaaccct cgatggtggt
     2281 gacctgcgcc cccccggccc cccgcgccag gtacaatgtg ctcaatccga agctcgtgcc
     2341 gagctccagg atggtttcag gcatgaagta ttcagcgagt cggaagagca tctgggcctg
     2401 ccgcgccggc ttgaggctgg tgcgggccat gtcgct
//
LOCUS       DAOQOC010000391         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25773_length_2433_cov_2.936922, whole genome shotgun sequence.
ACCESSION   DAOQOC010000391 DAOQOC010000000
VERSION     DAOQOC010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25773_length_2433_cov_2.936922"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PL106_07145"
     CDS             complement(<1..266)
                     /locus_tag="PL106_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32883.1"
                     /translation="MPVHHLRSVGLAASTLLLSASYAQLADPFTDGDFAADPAWAGID
                     ALFTVVDDGGNQRLRSNSPGAANYYLSTPNTLVDDARWEFFVDTR"
     gene            355..1362
                     /locus_tag="PL106_07150"
     CDS             355..1362
                     /locus_tag="PL106_07150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNA32884.1"
                     /translation="MRTAVVGATGLVGGMMLKVLEERKFPVDELLPVASERSSGRTVR
                     FRGREIPVIGMEEAVARRPDIALFSAGGGVSLEWAPRFAEVGCTVIDNSSAWRMEPDK
                     KLIVPEINGHLLGAADRIIANPNCSTIQLVMALAPLHRAFTAERLVVSTYQSVTGTGQ
                     KAVQQMDDERKDVVGEKVYPHPIDRNVIPRCDVFLANGYTKEEMKVVQETHKILDPAI
                     RVTCTAVRVPVTGGHSESVNVEFAKDFDLEEVRQLLRATPGIVLADDPANDVYPMPLI
                     ANGRDEVFVGRLRRDESHPRALNLWIVADNLRKGAATNAVQIAELLVANKLKTAERAV
                     Q"
     gene            1379..2254
                     /locus_tag="PL106_07155"
     CDS             1379..2254
                     /locus_tag="PL106_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32885.1"
                     /translation="MRTVSLLLALALLTSLRLNAQHPVDTTGRKLVRDVFVSAGGLVD
                     GGGWVTRGEVLGGAPGSTLLQGGLPDHTFEHGPAVLGGGLLHASIGLTLKENRAKGSS
                     MKLRTGLGMAWVGGASSSFERTERFPYDTLTSSGSGGTLYADSVSRSTYDIRHTHQRI
                     GLDIDLLFVKDFPGHWMLYGGAGVSVGTTLGGITRVEHRTSRYIETLDNGRDQGVYVT
                     EDESVRTSGVFFTNIHVPLGVGFRFARRNPFWRSLVLTWETRPGITIGGEPTNTPGPR
                     PGISFLLGMRVDLAR"
BASE COUNT          456 a          675 c          860 g          442 t
ORIGIN      
        1 cgggtatcca cgaaaaactc ccagcgcgca tcgtcgacca gggtgttcgg cgtgctcagg
       61 tagtaattcg ccgcaccggg gctgttgctg cgcaggcgct ggttcccgcc gtcgtccacc
      121 acggtgaaca gcgcatcaat tccggcccat gccgggtcgg cggcgaagtc accatccgtg
      181 aaggggtcgg ccagctgggc gtacgatgcg gagagcaaga gcgtggaagc tgcaaggcca
      241 acggatcgca ggtgatggac aggcatggtg cgaagctggt cggaaagatg ggggatccgg
      301 ggggaaccga tgcgcaacgg cgtatagctt cgtgcgctga aaacacgaag gaacatgagg
      361 acggccgttg tgggcgccac cgggctggtg gggggcatga tgctgaaagt gctggaggag
      421 cgcaagttcc cggtggatga actcctgccc gtggccagtg agcgcagctc gggtcgcacg
      481 gtgcggttcc gggggaggga gatccccgtg atcggcatgg aggaagctgt cgcacgccgt
      541 ccggatatcg ccctgttctc ggcgggtgga ggggtttcgt tggaatgggc cccccggttc
      601 gccgaagtag gctgtacggt gatcgacaac agcagtgcct ggcgcatgga gccggacaag
      661 aagctgatcg tcccggagat caatggacat ttgttggggg cggccgatcg gatcatcgcc
      721 aaccccaact gcagcaccat ccagttggtg atggcacttg ccccgcttca ccgggcattc
      781 acggccgaac gcctggtggt gagcacgtac cagagtgtta cgggcaccgg tcagaaagcg
      841 gtacagcaga tggatgatga acgcaaggac gtcgttggcg agaaggttta tccgcacccg
      901 atcgaccgga atgtgatacc gcgctgcgat gtctttttgg cgaacggcta caccaaggag
      961 gagatgaagg tggtgcagga aacgcacaag atcctcgatc cggcgatccg cgttacatgc
     1021 acggccgtgc gcgtaccggt gaccggcggc cacagcgaat cggtgaacgt ggagttcgca
     1081 aaggacttcg acctggagga ggtgcgtcag ttgttgcgtg cgacgccggg tatcgtgctg
     1141 gcggatgacc ccgcgaacga tgtgtacccc atgccattga tcgccaatgg ccgcgatgag
     1201 gtttttgtgg gccgtctacg cagggacgag agtcatccgc gagcgctcaa tctgtggatc
     1261 gtggccgata acctgcgcaa aggcgcggcc accaacgcgg tgcagatcgc ggagttgctc
     1321 gtggcgaaca agctgaagac cgctgagcgc gcggtccaat gaatggatat cccgaccgat
     1381 gagaaccgta tccctcctgc tggccttggc gctgctgacc tccctcaggt tgaacgccca
     1441 gcatcccgtc gatactaccg gacggaagct ggtccgggat gtgttcgttt cggccggagg
     1501 tctggtcgat ggcggtggtt gggtcacgcg cggtgaggtg ctcggtgggg ctccggggtc
     1561 gaccttgttg cagggcggct tgccggacca caccttcgag catggtccgg cggtactggg
     1621 cggggggctt cttcatgcct cgatcggcct gaccttgaag gagaaccggg cgaagggaag
     1681 ctccatgaag ctgcgcaccg gcctgggcat ggcctgggta gggggagcat cgtcgagctt
     1741 cgagcgcacg gagcgtttcc cgtacgacac gctcacgtct tcgggcagtg gtggaacgtt
     1801 gtacgctgat tcggtgagcc ggagcaccta cgacatccgg catacgcacc agcggatcgg
     1861 gttggatatc gatctgcttt tcgtgaagga ttttcccggg cactggatgc tgtacggcgg
     1921 agccggcgtt tcggtgggca ccacgctcgg tggcatcacg cgcgtggaac accggacctc
     1981 gcgctatatt gaaacgctgg acaacgggag ggaccaaggg gtgtacgtca cggaggatga
     2041 aagcgtccgc acgagcgggg tgttctttac caacattcat gtaccattgg gcgtgggctt
     2101 ccggttcgca cggcggaatc cgttctggcg ctcactggtg ctcacttggg aaacgcgacc
     2161 ggggatcacg atcgggggcg aacccacgaa tacaccgggg ccacgcccgg ggataagctt
     2221 cctcctgggt atgcgcgtcg accttgcgcg atagagcgac gaacttgacc cgggcgggag
     2281 gccgcccgtt ccccatcaag cacctgtatt ccggtccgct gaaaaggatg ccggggccga
     2341 tggccgggac ttccgggcag aatgataact gaacaccggt caggtggtcc cggggcccgc
     2401 cttatgtgga ggtacgctgt gcggcggtcc gct
//
LOCUS       DAOQOC010000392         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25795_length_2432_cov_3.299537, whole genome shotgun sequence.
ACCESSION   DAOQOC010000392 DAOQOC010000000
VERSION     DAOQOC010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25795_length_2432_cov_3.299537"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PL106_07160"
     CDS             complement(<1..1153)
                     /locus_tag="PL106_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNA32886.1"
                     /translation="MKELKPEMSNLYDRLVKKEAKLAVIGLGYVGLPIALEFARRIKV
                     VGFDINQKRVDMMRRGEDPSNELPAEAFKGADIHFSADLNDLKDVEFFIVAVPTPIDD
                     QNIPDLKPLLGATRTVGQVLKKGDHVVFESTVYPGCTEEDCVPLLEELSGLKFVTDFK
                     VGYSPERINPGDKQHTLSSIVKVSSGCDPESAETIARTYELVVKAGVHRAPSIKVAEA
                     AKIIENTQRDVNIALTNELSIIFNRMGINTFDVLEAAGTKWNFLKFQPGLVGGHCIGV
                     DPYYLVYKAKELGYHAQIIDSGRYVNDSMGGYVAKQTVKKIIAAGANPAEARVLVMGA
                     TFKENVTDIRNSKVADVVRELKSFSCAVDVADPHADSDEVKHEYGYELTK"
     gene            complement(1245..2258)
                     /locus_tag="PL106_07165"
     CDS             complement(1245..2258)
                     /locus_tag="PL106_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA32887.1"
                     /translation="MSSPIKFGVVGCGHIGKRHAEMVKRHPEAELVAVCDPLPASETK
                     ADQFGVPVFADLETMLRETPDLDVVNVCTPNGLHARQSLIALEARKHVVCEKPMGLSK
                     AECEEVLYKALQMHRTVFGVMQNRYSPPSQWIKSVIDQRLLGDIFLVQVNCYWNRDDR
                     YYKKGGWKGTGDLDGGTLFTQFSHFIDIMYWLFGDITDIHARFADFNHQGLTDFEDSG
                     LVTFRFVNGGMGCINYSTSVWDKNLESSLTVIGSKGSVKVGGQYMDQVEHCHIKDYVM
                     PSLAPTNPANDYGAYKGSANNHSYIIENVVDTIKGRSTLTTNALEGLKVVEIIERIYK
                     LKK"
BASE COUNT          394 a          753 c          715 g          570 t
ORIGIN      
        1 ccttggtcag ttcgtagccg tactcgtgct tcacctcgtc gctgtcggcg tgggggtcgg
       61 ccacgtccac ggcgcaactg aagcttttca actcgcgcac cacatcggcc accttgctgt
      121 tgcggatgtc ggttacgttc tccttgaagg tggcgcccat caccagcacg cgtgcctcgg
      181 cggggttcgc cccggcggcg atgatcttct tcacggtctg tttggccacg tacccgccca
      241 tgctgtcgtt cacgtatcga ccactgtcga tgatctgggc gtggtagccg agttccttcg
      301 ccttgtacac gaggtagtag gggtccacac caatgcagtg tccgcccacc aggcccggct
      361 ggaacttgag gaagttccac ttggtgcccg cggcctccag cacatcgaag gtgttgatgc
      421 ccatccgatt gaagatgatc gagagctcgt tcgtgagcgc gatgttcacg tcccgctggg
      481 tgttctcgat gatcttggcg gcctcggcca ccttgatgct gggggcgcga tgcacaccgg
      541 ccttcaccac cagctcgtag gtcctggcga tggtctcggc actttccgga tcgcagccgc
      601 tgctcacctt cacgatgctg gacagggtgt gctgcttgtc gcccggattg atccgttccg
      661 gtgagtatcc gaccttgaaa tcggtaacga acttcaatcc gctcagctcc tcgagcaggg
      721 gtacgcaatc ctcctcggtg caaccggggt acacggtgct ctcgaacacc acgtgatcac
      781 ccttcttcag cacctggccc acggtgcgcg tggcgcccag caagggtttc aggtcgggga
      841 tgttctggtc gtcgatcggc gtgggcacgg ccacgatgaa gaactccacg tccttcaggt
      901 cgttcaggtc ggcggagaag tggatgtccg cccccttgaa ggcctcagcg ggcagttcat
      961 tgctggggtc ctcgccccgg cgcatcatgt ccacccgttt ctggttgatg tcaaagccga
     1021 ccaccttgat gcggcgggcg aattccaatg cgatgggcag gcccacgtat cccaggccga
     1081 tcacggccag tttcgcctcc ttcttgacga gtctgtcgta caggttgctc atttccggtt
     1141 tcagttcttt caccacagag gtacggaggg cacagagttg aacgcctgga tccgtcagtg
     1201 gactttctcc gtgtcctctg tgcctctgtg gtgaactctg ttcctcactt cttcagtttg
     1261 tagattcgtt cgatgatctc caccactttc aggccttcga gcgcgttggt ggtgagcgtg
     1321 gagcgaccct tgatggtgtc caccacgttt tcgatgatgt agctgtggtt gttggcactg
     1381 cccttgtagg cgccgtagtc gttggcgggg ttggtgggcg cgaggctcgg catcacatag
     1441 tccttgatgt ggcaatgctc cacctggtcc atgtactggc cgccgacctt cacactgccc
     1501 ttgctgccga tcacggtgag gctgctctcc aggttcttgt cccacacgct ggtgctgtag
     1561 ttgatgcatc ccatgccacc gttcacgaag cggaaggtga ccaggccgct atcctcgaag
     1621 tcggtgagcc cctggtggtt gaagtcggcg aaacgggcat ggatgtccgt gatgtcgccg
     1681 aacagccagt acataatgtc gatgaagtgg ctgaattgcg tgaataatgt accgccatcc
     1741 agatcgccgg tgcccttcca gccgcccttc ttgtaatacc gatcgtcgcg gttccagtag
     1801 caattcacct gcaccaggaa tatgtcgccg aggagcctct ggtcgatcac gctcttgatc
     1861 cactggctgg gcggcgagta gcggttctgc atcaccccga atacggtgcg gtgcatctga
     1921 agggccttgt acagcacctc ttcgcattcc gccttgctca gccccatggg cttctcgcag
     1981 acgacgtgct tgcgggcttc cagcgcgatc aggctttgtc tcgcgtggag tccgttgggt
     2041 gtgcaaacgt tcaccacgtc caggtcaggc gtctcacgca acatggtctc caggtcggcg
     2101 aagacgggaa caccgaactg gtcggccttg gtctctgaag cgggcagcgg atcgcagacg
     2161 gccacgagtt cagcctccgg atggcgcttc accatttccg cgtggcgctt gccgatgtgc
     2221 ccgcagccca ccacgccgaa tttgatcggg gaactcatgc cagtcgcgcc acttttccgc
     2281 tggcgaggcg gtaccgctgt ccactctccg gacaggtcgc ttcaccgttc gtatcgaatt
     2341 tcaacttgtg gccgaactcg ctcatccaac ccgtttggcg ggcgggattg cccaccacga
     2401 gcgcatggtc cggcacttcc ttggtgacca cc
//
LOCUS       DAOQOC010000393         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25868_length_2428_cov_3.680995, whole genome shotgun sequence.
ACCESSION   DAOQOC010000393 DAOQOC010000000
VERSION     DAOQOC010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25868_length_2428_cov_3.680995"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /gene="folK"
                     /locus_tag="PL106_07170"
     CDS             complement(<1..255)
                     /gene="folK"
                     /locus_tag="PL106_07170"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNA32888.1"
                     /translation="MEALLLLGGNLGDPVDTLARADALLSELTGRVLARSRDHWTEPH
                     GFADDRLFLNRAVLVNTELAPLDLLNRCLSIETQLGRTRTP"
     gene            451..2280
                     /gene="sppA"
                     /locus_tag="PL106_07175"
     CDS             451..2280
                     /gene="sppA"
                     /locus_tag="PL106_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNA32889.1"
                     /translation="MGQFFKFMFASMFGFILGSLVIGLIFAVLIFGAIGAAAGSFNFE
                     GKPVTVKDRSILHMELVQTIVDRGDKDELMFDFGPFKQASRLGLNDILADLEKAKTDD
                     RIKGVFMDLGFSVNARMATVQEIRDKILEFKKESGKPVIAFAELYTQGTYYLASAADE
                     VYLVPQGDLDFRGLQAEMMFLKGLFDKLGIEMQFIRGSNNRFKSYGETFILKEMSPEN
                     ERQMSELIGGLWDQYLTVVGASRNIDKAKLNEVAEKLVVREAKDAVGAGMIDGLKYRD
                     EVITLMKEKMGLDTDEDLRLLSLAKYARASMPVKAGEKTVSTYNKPKLAVVYAQGDIN
                     SGESEEGSIGSTTIAEALRQAREDSTVKAIVFRVNSPGGSGLASDVIWREVTLAKAVK
                     PVVVSMGDLAASGGYYISCAADRIYAQPNTITGSIGVFGIIPNMKGFFNDKLGITFDG
                     VKTNTYADMLTTSRPLNADEKAIFQGYVDKFYETFKSRVAEGRKLPIARVDSIGQGRV
                     WTGTRAKEIGLVDEIGGLEAAIADAAQRANLAEGDYRTVDYPEQEPLFQQILKNLDGN
                     AKAWMREEAFGDDVELLHQFEAVRKARNMAGVQARMPFDVDVH"
BASE COUNT          503 a          723 c          795 g          407 t
ORIGIN      
        1 cggcgtacgc gtacgaccga gctgcgtttc gatggagagg catctgttca agaggtccag
       61 tggagcaagt tccgtgttca ccagaactgc ccggttcagg aaaagacgat cgtccgcgaa
      121 accatggggt tcggtccaat gatcccggct tcgcgccagc acccgaccgg tgagttcgct
      181 cagcagggcg tcagcacggg ccagtgtgtc caccggatcg cccaggttac cacccaacaa
      241 caggagcgcc tccatcgcgt ggcgaaacta tcggccgcag gagttcttgg ttcgcagcgg
      301 acaggatgga gcgctcgggt acccggaagg acaacggtgc agcggcgttc ctgggccttt
      361 ccgtcaggcg cagccgccgt ccatgtagaa cacccgccca accccgtcag tgggcctact
      421 ttcgaccggt caagaaacga cacgtacgcc atgggacagt tcttcaagtt catgttcgct
      481 tcgatgttcg ggttcatcct gggctcgctg gtcatcgggc tcattttcgc cgtcctcatt
      541 ttcggtgcca tcggtgcggc cgctggcagc ttcaacttcg agggcaagcc cgttaccgtg
      601 aaggacagat cgatcctgca catggaactc gtgcaaacga tcgtggaccg gggcgacaag
      661 gatgagttga tgttcgattt cggcccgttc aagcaagctt cccggttggg tctcaatgat
      721 atcctggccg acctggagaa agccaagacc gacgaccgga tcaagggcgt gttcatggac
      781 ctcggtttct cggtgaacgc ccgcatggcc accgtgcagg agatccgcga caagatcctt
      841 gaattcaaga aggaaagtgg caagcccgtg atcgcgttcg ccgagttgta cacccagggc
      901 acgtactacc tggccagcgc ggccgacgag gtgtacctgg tcccgcaagg cgacctcgac
      961 ttccggggcc tgcaggccga gatgatgttc ctgaaaggac tcttcgacaa gctggggatc
     1021 gagatgcagt tcatccgcgg cagcaacaac cgtttcaaga gttacgggga gaccttcatc
     1081 ctgaaagaga tgagccccga gaacgagcgg cagatgagcg aactgatcgg ggggctctgg
     1141 gaccagtacc tcaccgtggt gggggcctcg cgcaacatcg acaaggcgaa gctcaacgag
     1201 gtggccgaga agctcgtggt ccgcgaggcg aaggatgccg tgggcgccgg catgatcgac
     1261 ggattgaaat accgcgatga agtgatcacg ttgatgaagg agaaaatggg gctcgatacg
     1321 gacgaggacc tgcgcctcct gagcttggcg aaatacgccc gtgcgtccat gccggtgaag
     1381 gctggcgaga agaccgtcag cacgtacaac aagcccaagc tggccgtggt gtacgcgcag
     1441 ggcgacatca acagcgggga gagcgaggag ggcagcatcg gcagcaccac catcgccgag
     1501 gccctgcggc aggcgcgcga ggacagcacg gtgaaggcca tcgtcttccg cgtgaacagc
     1561 cccggcggca gcggcctggc cagcgacgtg atctggcgcg aggtcaccct ggccaaggcg
     1621 gtgaagcccg tggtggtgag catgggcgac ctggccgcca gcggcgggta ttatatcagt
     1681 tgcgcggccg accgcatcta tgcccagccc aacaccatca ccggcagcat cggggtgttc
     1741 ggtatcattc ccaacatgaa gggattcttc aacgacaagc tcggcatcac gttcgatggg
     1801 gtgaagacca acacctacgc cgacatgctc accacctcgc ggccgctgaa cgccgatgag
     1861 aaggccatct tccagggata cgtggacaag ttctacgaga ccttcaagag ccgtgtggcc
     1921 gagggtcgca agctcccgat agcgcgggtg gacagcatcg gacagggccg cgtgtggacc
     1981 ggcacccgcg ccaaggagat cgggctggtg gacgagatcg gggggctgga ggccgccatt
     2041 gccgacgctg cccaacgcgc gaacctggcc gaaggcgact accgcacggt ggactacccc
     2101 gagcaggagc cgctcttcca gcagatcctg aagaacttgg acggcaacgc gaaagcttgg
     2161 atgcgggagg aagccttcgg cgacgacgtg gaactgctgc accagttcga ggcggtgcgc
     2221 aaggcccgca acatggccgg ggtacaggcc cgcatgccct tcgacgtgga cgtgcattga
     2281 ggcgcgaatg atcaggtgcc tcctgttgtc atacttcggc gggctcagca tgacaacagg
     2341 gggcttccgc ctgttctgcg cgccccaagc agtaagggcc ccggttgccc ggagccctcc
     2401 accctgtgtg tcgtcggtgt ccgggagc
//
LOCUS       DAOQOC010000394         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_25967_length_2423_cov_4.200169, whole genome shotgun sequence.
ACCESSION   DAOQOC010000394 DAOQOC010000000
VERSION     DAOQOC010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25967_length_2423_cov_4.200169"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PL106_07180"
     CDS             complement(<1..725)
                     /locus_tag="PL106_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNA32890.1"
                     /translation="MQRSVLLLPLVLCSAIVTAQERTLIGFQLGNHLSKADPERMMSL
                     YLEGPTSEVGRVVKEHGGTVVMKMRNWTSVRIPAGRVHELDQEPAVRSIQGYGFGQTL
                     NDSMRVKTHIDLAQQGMSPLPRGYDGSGVVMGIVDTGIDLNHPDMRDSTGTRVLHFWD
                     HHQVTADNTPPEFGFGQEWSKEEIDNGDCPLQFTNYAGESDAGGHGTTVAGTAAGNGG
                     NTGHYIGAAPKADLIIVSAVTIN"
     gene            complement(765..1232)
                     /locus_tag="PL106_07185"
     CDS             complement(765..1232)
                     /locus_tag="PL106_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNA32891.1"
                     /translation="MQRLNLPDHGVKLRHDADGDRVWDPLRKKWLVLTPEEWVRQHVL
                     NHLVHDLGCPVTLVSAEHTIVLNGLTKRADLVVHGREGRPLLLVECKAPHVRMDRKVF
                     EQAARYNLVFRVPYLLVSNGLVHYCCRVDHVSGAVDFLPKVPAYAAMVGGDRT"
     gene            1257..2030
                     /locus_tag="PL106_07190"
     CDS             1257..2030
                     /locus_tag="PL106_07190"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNA32892.1"
                     /translation="MNSKEEIVHNWLPRYTGVALDAFKPHILLTNFSNYLDLFVQQTG
                     ATVIAHDKPIQVAIAKDMCMINFGMGSPMAATTMDLLSAIRPQAVLFLGKCGGLKRKN
                     ELGDLILPIAAIRGEGTSNDYFPPEVPALPSFMIHRAVSGVIRDMELDYWSGTVYTTN
                     RRVWEHDDRFKDRLRTMRCMGIDMETATIFMTGFANEIPTGALLLVSDQPMVPHGVKT
                     SASDSKVTVSYAERHVRIGVESLRDIINQGRSVKHLRFE"
     gene            2130..>2423
                     /locus_tag="PL106_07195"
     CDS             2130..>2423
                     /locus_tag="PL106_07195"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32893.1"
                     /translation="MSTTDDFKKIVKDIRAKSFKPVYFLHGEEGFFIDRVEEEIVKAA
                     LEEHERDFNLSILYGKDTAPDQVKDACLRYPMMAERQVVVLREAQTWKIDAFDK"
BASE COUNT          485 a          810 c          701 g          427 t
ORIGIN      
        1 ttgttgatcg tgacggcgga gacgatgatg aggtcggcct tcggcgccgc gccgatataa
       61 tggcccgtgt tgccaccgtt gcccgcggct gttcccgcca cggtggtacc gtgcccgcca
      121 gcatcggact caccggcata gttggtgaac tgcagcgggc agtccccgtt gtcgatctcc
      181 tccttgctcc actcctgccc gaacccgaat tccggcgggg tgttatcggc cgtcacctgg
      241 tgatggtccc agaagtgcaa tacccgcgta ccggtactgt cgcgcatgtc ggggtggttc
      301 aggtcgatcc cggtgtccac gatgcccatc accaccccgc tcccatcgta cccgcggggc
      361 agtggcgaca tcccttgctg ggccagatcg atatgcgtct tcacgcgcat gctgtcgttc
      421 agcgtctgcc cgaagccgta tccctgtatg ctgcgcacag cgggttcctg gtccagctca
      481 tgcacccgac ccgccgggat ccgcacgctc gtccagttgc gcatcttcat caccaccgtg
      541 cccccatgtt ccttcaccac ccgccccacc tccgatgtgg gcccttccag atagaggctc
      601 atcatccgtt ccggatcggc cttgctcagg tggttgccga gttggaaccc gatcaaggtg
      661 cgttcctggg ccgtaacgat cgcgctacaa agcacaagcg ggagaaggag tactgaacgt
      721 tgcatggatg tggggagccg ggatgcgcgg cgaccggacc aaaattaggt gcggtccccg
      781 cccaccatcg ccgcgtatgc cgggactttc ggcaagaaat caacagcacc cgaaacgtgg
      841 tccacccggc agcaatagtg gaccaggccg ttcgacacca gcaggtaggg cacgcggaac
      901 accaggttgt agcgcgccgc ctgctcaaag accttccggt ccatccgcac gtgaggcgcc
      961 ttgcactcca ccagcaacaa cggcctgccc tcgcggccat gcaccaccag gtccgcacgt
     1021 ttggtcaggc cgttcaatac gatggtgtgc tcggcggaaa ccagtgttac cgggcagccc
     1081 agatcgtgta caaggtgatt cagtacatgc tgccgcaccc attcctcggg cgtgagcacc
     1141 agccacttct tgcgcaaggg atcccacaca cggtcgccat cggcatcgtg cctaagtttg
     1201 acgccatggt ccggaaggtt caaccgttgc ataccgggca ctaagaagga acaaaagtga
     1261 acagcaagga ggagatcgta cacaactggc ttccgcgcta cactggcgtg gccctggatg
     1321 cgttcaagcc gcacatcctg ctcaccaact tcagcaacta cctggacctc ttcgtgcagc
     1381 agaccggtgc cacggtgatc gcgcacgaca agcccatcca agtggccatc gccaaggaca
     1441 tgtgcatgat caacttcggc atgggcagcc ccatggccgc caccaccatg gacctgctca
     1501 gcgccatccg cccccaagcg gtgctcttcc tgggcaagtg cggcggcttg aaacgcaaga
     1561 acgaactggg cgacctcatc ctgcccatcg ccgccatccg tggcgagggc accagcaacg
     1621 actacttccc gcccgaagtg cccgcgctgc cctccttcat gatccaccgc gccgtgtccg
     1681 gcgtgatccg cgacatggaa ctcgactatt ggagcggtac cgtttacacc accaaccgcc
     1741 gggtgtggga gcacgatgat cgcttcaagg accggctgcg caccatgcgc tgcatgggca
     1801 tcgacatgga gacggccacc atcttcatga ccggtttcgc caacgagatc cccaccggcg
     1861 ccctgctcct ggtgagcgac cagcccatgg tgccccacgg cgtgaagacc agcgccagcg
     1921 acagcaaggt gaccgtcagc tacgccgaac gccacgtgcg catcggggtg gaaagcctgc
     1981 gcgacatcat caaccagggg cgcagcgtga aacatctgcg attcgagtga tcccatgcgg
     2041 atgatccagt acacgggtgc gcgtttgcca cgaccttccc cacgcacgaa catcggccgc
     2101 catcaacgca tgaacagcgc attccacgga tgagcaccac cgacgacttc aagaagatcg
     2161 tgaaggacat ccgggcgaag tcgttcaagc cggtctattt cctgcacggt gaagagggtt
     2221 tcttcatcga ccgcgtggaa gaggagatcg tgaaggccgc gctggaggag catgagcgcg
     2281 acttcaacct gagcatcctg tacggcaagg acaccgcgcc cgaccaggtg aaggacgcct
     2341 gcctgcgtta ccccatgatg gccgaacggc aggtggtcgt gctgcgcgag gcccagacct
     2401 ggaagatcga tgcgttcgat aag
//
LOCUS       DAOQOC010000395         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26076_length_2418_cov_2.587812, whole genome shotgun sequence.
ACCESSION   DAOQOC010000395 DAOQOC010000000
VERSION     DAOQOC010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26076_length_2418_cov_2.587812"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PL106_07200"
     CDS             <1..275
                     /locus_tag="PL106_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32894.1"
                     /translation="PVESGEVLVSKLVDRTVTDQARYAGYDGQPGELYPSRNGVVFND
                     GYRDLQNQLRASRTVKAPGVLGSDALRQVTEGVAWEVQGAIANRIP"
     gene            272..1648
                     /locus_tag="PL106_07205"
     CDS             272..1648
                     /locus_tag="PL106_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNA32895.1"
                     /translation="MTRIYFYTLMLLVACKGRQGTVMNEPVAEVSRPAWVLARPVDDL
                     DYIGIGSCPKARADYQESAKKNALNDLASEISVRVEGNSLLSSLDSRTRFTETYQSNI
                     RTTSNEQLEGFEMVDSWQDAREYWVYYRLDKSVHARIKAERKEKALAIARDGFGRAQE
                     RLAAGDLKGAFDQDLRALIAMKEYWGENDQVTVGDRQVPLANAIFADLQRLTSSVRFA
                     PLPERCLLDYSDRFAREMLITASFSDGTLARRLVQLPVTFSWPGTSGRVSESRGTDEE
                     GHVRATVKHVEMNGATADLLVRLNMDEMVSPELDPVFVRPVVSSLTVPEVHVPIDLTM
                     PRLHVTSNETNLGQPVSAGIAIGIKEEVTAKGFRIVGRPADADLLLDLRASTREGGQS
                     NGFFTTLLDVTWSFRDRRTNDVVVEGGRQGVKGIQLDHVKAGLDAYKKAGNDLRKELV
                     SSMLNTLL"
     gene            1735..2337
                     /locus_tag="PL106_07210"
     CDS             1735..2337
                     /locus_tag="PL106_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32896.1"
                     /translation="MKTMTTNRTLSIALTALLLAGGLGACKPKKDKAKAETVKPPSGE
                     TEVNVLCSGPEFFTNDKVFRANNLGESMDQATSKKKALANARADLASAIQTQIKGVID
                     NYVNSRELNNKEEVAERFEGLTREVIDQKLVGVKTICEKQVVVNATGNYKTYVAIELS
                     AQDLLAAYNERLSKDERLRIDYDYEKFKETFDKEMEKMGK"
BASE COUNT          533 a          704 c          786 g          395 t
ORIGIN      
        1 tgcccgtgga gtcgggcgaa gtgctggtga gcaagctggt ggaccgcacg gtgaccgatc
       61 aggcccggta cgcgggttac gacgggcagc ccggtgaact gtatccatcg cgcaacggtg
      121 tggtcttcaa cgatggctac cgcgacctgc agaaccagct cagagcctcc cgcacggtga
      181 aagcccccgg ggtgctcggc tccgacgcgt tgcgccaggt cacggaaggc gtggcatggg
      241 aggttcaggg cgcgatcgcg aacaggatcc cgtgacacgc atatatttct atacgctgat
      301 gctcctggtc gcctgcaaag gccgccaggg aacggtcatg aacgagccgg tggccgaagt
      361 gtccaggccg gcgtgggtgc ttgcacgccc ggtggacgat ctggactaca tcggcatcgg
      421 aagctgcccg aaggcacggg cggactatca ggaaagtgcg aagaagaacg cgctgaacga
      481 cctggccagc gagatcagcg tgcgcgtgga gggcaacagc ctgttgtcat cgttggattc
      541 gcggacgcgg ttcaccgaga cgtaccagag caacatccgc accacgagca acgaacagct
      601 cgagggcttc gaaatggtgg acagctggca ggatgcccgc gagtactggg tgtattaccg
      661 gctcgacaag agcgtgcatg cgcggatcaa ggccgaacgc aaggagaaag cattggcgat
      721 cgcccgggac ggcttcgggc gggcgcagga gcgactggcg gccggtgacc tgaaaggggc
      781 ctttgaccag gacctgcggg cattgatcgc catgaaggag tattgggggg agaacgacca
      841 ggtgaccgtc ggggatcggc aggtgccgtt ggccaacgcc atcttcgccg acctgcaacg
      901 cctcaccagc agtgtgcggt tcgcgccgct ccctgagcgc tgcctgctcg attattccga
      961 tcggttcgcc cgcgagatgc tgatcacggc ctcattttca gacggcaccc tggcccgtcg
     1021 cctggtgcaa ctgcccgtga ccttcagctg gccgggcacc agtggccggg tgagtgaaag
     1081 ccgcggtacg gacgaggagg gacatgtaag ggccacggtg aagcacgtgg agatgaacgg
     1141 ggccaccgct gatctgctgg tccgcctgaa catggatgag atggtgagcc ctgagcttga
     1201 tcccgtcttc gtgcggccag tggtttccag cctcactgtt ccggaggtcc atgtgcccat
     1261 cgacctgaca atgccccggt tgcacgtcac ttccaacgag acgaacctgg gccaaccggt
     1321 gtccgcaggg atcgcgatcg gcatcaagga ggaggtgacg gccaaaggct tccgcatcgt
     1381 tggtcgcccg gccgatgcgg accttctgct cgacctccgg gcctctaccc gggaaggagg
     1441 gcagtccaac ggtttcttca ccacgctcct cgatgttacc tggtccttcc gcgaccggcg
     1501 gaccaacgac gtggtggttg agggaggccg acagggcgtg aagggcatcc aactggacca
     1561 tgtgaaggca gggctggacg cgtacaagaa ggccggtaac gacctgcgca aggaactcgt
     1621 cagcagcatg ctcaacactt tgttgtagga tcgacgcgca atgcccaagt ttggcacacc
     1681 gttcgcaaaa gccccctcgg aacatcacaa caacgcagaa cacaagcccc gaacatgaag
     1741 accatgacca ccaaccgcac gctctccatc gcgctcaccg ctctgctgtt ggcgggtgga
     1801 ttgggtgcct gcaaacccaa gaaggacaag gccaaggccg aaaccgtcaa gccgcccagt
     1861 ggcgagaccg aagtgaacgt gctctgctcc ggccctgaat tcttcaccaa cgacaaggta
     1921 ttccgcgcca acaacctggg tgaaagcatg gaccaggcca ccagcaagaa aaaggccctg
     1981 gccaacgccc gtgccgatct ggccagcgcc atccaaacgc agatcaaagg cgtgatcgac
     2041 aactacgtga acagccgtga gctgaacaac aaggaagagg tggccgagcg cttcgagggc
     2101 ctcactcgcg aggtgatcga ccagaagctc gttggcgtga agaccatttg cgagaagcag
     2161 gtggtggtga acgcgacggg caactacaag acctatgtgg ccattgaact gagtgcacag
     2221 gatctgttgg ccgcgtacaa cgagcgcttg agcaaggacg aacgcctgcg tatcgattac
     2281 gactacgaga agttcaagga gaccttcgac aaggagatgg agaagatggg caagtaaagc
     2341 ctgagctcac tggatatcgg accccggtca atggccgggg tccgtgcttt tgtggatgga
     2401 cggggatcga tcaatacc
//
LOCUS       DAOQOC010000396         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26187_length_2412_cov_3.514213, whole genome shotgun sequence.
ACCESSION   DAOQOC010000396 DAOQOC010000000
VERSION     DAOQOC010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26187_length_2412_cov_3.514213"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PL106_07215"
     CDS             complement(<1..396)
                     /locus_tag="PL106_07215"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNA32897.1"
                     /translation="MERITESTSRYDHLERMTTDELLRNINSEDRTVADAVAETLPGL
                     ARLVDAIVERMRAGGRLFYLGAGTSGRLGIVDASECPPTFGVPHGLVTGLIAGGDTAI
                     RKAVEHAEDDHEQAWKDLQEHAIGPADTVI"
     gene            451..1986
                     /locus_tag="PL106_07220"
     CDS             451..1986
                     /locus_tag="PL106_07220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA32898.1"
                     /translation="MDPLFLIGRMGRRSSLRDVEGQGQMARFRADVRSGWKELRRTTS
                     NSHKWLVFAVVAAGLVLRLARLGDGITYDEAFTASFYASRTTGFLLSDYSWPGNHLLF
                     TLLVKLTMQLFGTHLWSLRLPALLAGVCVLPLFYLFVRQMFNRYVAVLALSFVAASGA
                     LIEYSALARGFSLSWLFMVAAWVAGRHLAKRNNTVSALLVALFCALGMWAAPAMLPAA
                     LSTYLWLLLYLLSLYKTSLNRRLPLLLLSFTVFIVLTALAYTPVVMTHNLGHLLRHPA
                     LGEYDWAQFTRTHQDRAAALWVFLNDTTNLWLSLILFIGMAYAAYISAKYRSMLIALI
                     GGCVPLVLIRASVGAPEGWAFLLFNVYIGTALALFYLLKFVQENIHAGFGKRFRTALA
                     CALVLMGLGWAGITRHVDHGHRFVDVPKAAAWFKGTLHPGDRVRVAPPADAPFKFHLR
                     AEGMDPAYVNATGGNGRLYALVSPAEGQTVGRVIKGDPRSADSTAMVKVMDWRRVEIW
                     LRP"
     gene            2162..>2412
                     /locus_tag="PL106_07225"
     CDS             2162..>2412
                     /locus_tag="PL106_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA32899.1"
                     /translation="MADEGRQVIFNMVKVGKTLPSGKKILNDIYLGFYYGAKIGILGL
                     NGSGKSTLMRIIAGKEKSFEGDIHVTPGFSIGHLEQEPEL"
BASE COUNT          406 a          729 c          770 g          507 t
ORIGIN      
        1 gatcaccgtg tcggcgggac cgatggcatg ttcctgcagg tctttccagg cttgttcgtg
       61 atcgtcctcc gcatgttcca cggccttgcg tatggcggta tctccacccg cgatcaggcc
      121 ggtgaccagg ccatgcggta ccccgaaggt gggcgggcat tcgctggcat ccacgatccc
      181 gagacggcca ctggtacctg caccaaggta gaacaacctg ccgcccgccc tcatccgctc
      241 cacaatggcg tccaccaggc gggcaaggcc gggcagggtc tccgccaccg catcggccac
      301 ggtgcggtcc tccgagttga tattgcgcag cagttcgtcg gtggtcatcc gttccaggtg
      361 atcgtaccgc gaggtgcttt cggtgatgcg ttccatgcca aggatgggct tgtggggcga
      421 aagtggtgcg ccgggcggtt caagcaatgg atggaccccc tatttttgat cggccgcatg
      481 gggagaagaa gttcgctgcg cgacgtggag gggcagggcc agatggcccg gttccgtgcg
      541 gatgtgcgga gcggctggaa ggaacttcgc cgcaccacga gcaattccca caaatggctg
      601 gtgttcgccg ttgtagcggc tggtctggtc ctgcgcttgg ccaggctcgg ggatggcatc
      661 acgtacgatg aggccttcac ggcctccttc tatgcgtcgc gtacgacagg cttcctcctc
      721 agcgactaca gttggccggg aaaccatttg ttgttcacgc tgctggtcaa actgaccatg
      781 caactcttcg gaacgcactt gtggagcctg cgtctaccgg ccttgctggc cggggtttgt
      841 gtcctgccgc tgttctacct cttcgtccgg cagatgttca accgctatgt ggcggtgctg
      901 gcactgtcgt tcgtggcggc cagcggtgcg ctcatcgagt acagtgcgct ggcccggggc
      961 ttcagcctgt cctggttgtt catggtggcg gcatgggtgg ccggtcgtca cttggccaag
     1021 cgcaacaaca cggtgagtgc attgctcgtt gcgctgttct gtgcgctggg catgtgggcc
     1081 gcgcccgcca tgctgcccgc tgcattgagc acgtacctgt ggttgttgct gtacctcttg
     1141 tccttgtaca agaccagcct gaaccggcgg ctgcccctgc tgctcctgtc tttcacggtt
     1201 ttcatcgtgc tgaccgcact ggcgtacacc cccgtggtga tgacgcacaa cctgggccat
     1261 ttgctgcggc atcccgcttt gggcgaatac gattgggcgc aattcacccg cacgcaccag
     1321 gaccgggcgg ccgccttgtg ggtgttcctg aacgatacga ccaacctctg gctcagtctg
     1381 atcctgttca tcggcatggc gtacgcggca tacatcagcg cgaagtaccg cagcatgctc
     1441 atcgccctga tcggtggctg cgtaccgctg gtgctgatcc gtgcttcggt gggggcgccg
     1501 gaaggatggg cctttctcct cttcaatgtc tacatcggca cggcactcgc gttgttctat
     1561 ctgctgaagt tcgtgcagga gaatatccat gcgggcttcg gcaaacggtt ccggaccgca
     1621 ctggcctgtg cgctggtgct catgggcctt ggttgggctg gcatcacccg gcatgtggac
     1681 cacggtcacc ggttcgtgga tgtgccgaag gcggccgcat ggttcaaagg cacgcttcat
     1741 cccggcgacc gtgtccgggt ggctccaccg gccgatgccc cgttcaagtt ccacctcagg
     1801 gcggaaggaa tggatccggc ctatgtgaac gccaccgggg gcaatggtcg gctgtacgcc
     1861 ttggtgagcc cggcggaagg ccagacggtg ggaagggtga tcaagggtga tccccgatca
     1921 gccgacagca ccgcgatggt gaaagtgatg gattggcggc gcgtggagat ctggctcaga
     1981 ccatagcgcc atccaagccg cccggtggtc ctggagggcc gctccggtca tcgttgttcg
     2041 tcaattgctg ttcaggagcg aacttcgcgg ccctttccga acgcgacgat ccaataatcc
     2101 ggggcgacga gatcgtcgcc ccaattcaaa atccaaggcg acgtgaacgt cgccccaact
     2161 catggcagac gaaggcagac aggtgatctt caacatggtg aaggtgggca agacgctccc
     2221 ttccggcaag aagatcctca acgacatcta cctcggcttc tactacgggg ccaagatcgg
     2281 gatcctgggt ctgaacggct cgggtaaatc cacactcatg cggatcatcg ccgggaagga
     2341 gaagtccttc gaaggcgaca tccacgtgac gcccggcttc agcatcgggc acctggagca
     2401 ggagcccgaa ct
//
LOCUS       DAOQOC010000397         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26263_length_2409_cov_3.783772, whole genome shotgun sequence.
ACCESSION   DAOQOC010000397 DAOQOC010000000
VERSION     DAOQOC010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26263_length_2409_cov_3.783772"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PL106_07230"
     CDS             complement(<1..287)
                     /locus_tag="PL106_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32900.1"
                     /translation="MNRRWVLIAIGYAWSLGTMAQESASGTVASIRTVPDSGYFWPGR
                     PVDHQLVFRTRAMYDASSIRNELVSALWHGGNIDRELRERSANNGGGPERAG"
     gene            complement(280..1905)
                     /locus_tag="PL106_07235"
     CDS             complement(280..1905)
                     /locus_tag="PL106_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32901.1"
                     /translation="MILRYWTLAAARTALLALALSACQKAADETRWDVDVAVPLVRVT
                     LDIGDLLGDTLISADGQGNVSLVHTARLFELGLDTLLELPDTMIRLRYPEYNIPPGST
                     FDLTPGAPFTGSDQSTHFDLQDLQLRELRVRSGTLGMAFVNRVGSVLNGQFALPGATL
                     GGAPLAWSPSIPAGSSSAPATLSQNFPLNGYSIDLRGPGYNEVNTLAMDLSLTNASAV
                     TIGSADSVEAFVSYHDIEPRYVRGYFGQHELDIDPDTSELDAFDGLSGLLDLDQVTAT
                     LRITNGIGMDARARINSIHSLNSATGIVVPLTHTITAGPLNLDRALDLGNTFQPAVNS
                     WTMNNGNSNIDAFIENLPDAIAYDLDVTADPLGDVSNGNDFLYDDSRITADLDVDIPL
                     NIIATNLTLSRRMPVELSGTLEHHALQSGTLHVFVENRFPFSAQVMLSQETQEGTVIN
                     TIGTGGTIASAQVDPDGHVIASTGSQLDFNLDPGQVDLFYPGSSAGPDGARLRFTIVF
                     NTADQAQHLRIRTDHTISIAVSLEGNYIVNGDE"
     gene            1986..>2409
                     /locus_tag="PL106_07240"
     CDS             1986..>2409
                     /locus_tag="PL106_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32902.1"
                     /translation="MGLQRRILVPLSLSALVMFGLSWVWHGLALNDLRDLTIPLGLYI
                     ALAAVAYLIIGAILTFSIHHAMANGWIDIRRAFPLKAMLYGAVVGFVVYLIALVFGMA
                     FTGGKLMHMVVDVLWQMVEQCLGGLMVSLGIIYDLHKSF"
BASE COUNT          435 a          705 c          757 g          512 t
ORIGIN      
        1 cccgcacgct ccggtccacc accgttgttc gcggaacgtt cgcgcagttc gcgatcgatg
       61 ttcccaccgt gccacagggc gctcaccaac tcgttgcgga tggaggacgc atcgtacatc
      121 gcccgtgtgc ggaacaccaa ttgatgatcg accggtcggc caggccagaa gtaaccactg
      181 tccggaactg tacggatgga tgccacggtc ccgctggccg attcctgggc catcgtgccg
      241 agcgaccatg catatccgat ggcgatgagg acccaccgcc tattcatccc cattcacgat
      301 gtagttccct tccaaggata ccgcaatgct gatcgtgtgg tccgtacgga tccgcagatg
      361 ttgtgcctgg tcggcagtat tgaaaacgat cgtgaagcgc agccgtgcac catcgggacc
      421 tgcggaggat ccggggtaga agaggtccac ttgccccgga tcgaggttga agtcgagctg
      481 cgaaccagtg ctggctatca cgtggccatc ggggtccacc tgcgccgagg cgatggtgcc
      541 tcccgttccg atggtattga tcaccgttcc ttcctgtgtt tcctggctga gcatgacctg
      601 ggccgagaac gggaaccggt tctccacgaa cacgtgcagg gtgccgctct ggagggcgtg
      661 atgttcaagt gtgccgctga gttccaccgg catgcgcctg gagagggtga ggttcgtggc
      721 gatgatgttc aacgggatgt ccacgtccag atcggccgtg atccggctgt cgtcgtacag
      781 gaagtcgttc ccgttgctca catcgcccag tggatccgcc gtcacatcca ggtcgtacgc
      841 gatggcgtcc ggcagattct cgatgaaggc gtcgatgttg ctgttgccgt tgttcatcgt
      901 ccaactgttc accgctggtt ggaaggtatt cccgaggtcg agcgcacggt cgagattgag
      961 cggccctgcg gtgatggtgt gcgtgagtgg cacaacgatc ccggtggcac tgttcaatga
     1021 atggatgctg ttgatgcgtg cgcgtgcgtc catgccgatg ccgttggtga tgcgcagggt
     1081 ggccgtgacc tggtccagat cgagcaggcc gctcaaacca tcgaacgcat caagctcgct
     1141 cgtatccgga tcgatgtcca actcgtgctg accgaaatat ccgcgcacgt agcggggctc
     1201 gatgtcgtgg tagctgacga aggcttccac actgtccgcc gaaccgatcg tgaccgctga
     1261 ggcgttggtg agtgaaaggt ccatggcgag tgtgttcacc tcgttgtatc ctggcccgcg
     1321 cagatcaatg ctgtagccgt tcagggggaa gttctgcgac aacgtggccg gtgcggagga
     1381 tgaacccgcc gggatcgacg gggaccaggc cagcggtgcg ccaccgagtg ttgcgccggg
     1441 cagcgcgaac tgcccgttga gcactgatcc cacccggttg acgaacgcca tgccaagcgt
     1501 gcccgaacgc acgcgcagtt cccgcaactg cagatcctgc agatcgaagt gcgtgctctg
     1561 atcagaaccc gtgaaggggg cgcctggagt gaggtcgaag gtggagcccg gggggatgtt
     1621 gtattccggg tagcgcaacc ggatcatggt gtccggcaat tccagcaagg tgtccagccc
     1681 caattcgaag agacgtgccg tgtgtacgag actcacattt ccctggccat ccgcactgat
     1741 caacgtatca cccaggaggt cgccgatatc caaggtcaca cgcaccaacg gtacggccac
     1801 atccacgtcc caacgtgttt cgtccgctgc tttctggcag gccgaaaggg ccagcgccag
     1861 gagtgccgtg cgagcggccg caagcgtcca gtaacgcaag atcatggggt ggaaaggtag
     1921 gtgacgccgg tcaacgccgt tttcgtgccg gccatcgtag attcgccttc gttcctgtcc
     1981 tggtcatggg ccttcagcgc cgcattttag ttcccctcag cctcagcgca ctggtgatgt
     2041 tcggtctgtc gtgggtctgg cacggcttgg ccctgaacga cctgcgcgac ctgacgatcc
     2101 ccttgggatt gtacatcgca ctcgcggctg tcgcctatct gatcatcggt gcgatcctca
     2161 cattcagtat ccatcacgcc atggcgaacg gatggatcga tatccgtcgt gcattcccgt
     2221 tgaaggcgat gctgtatggt gccgttgtcg ggttcgtcgt ctatctcatc gccctggtct
     2281 tcggcatggc tttcaccggc ggcaaattga tgcacatggt ggttgacgtg ctctggcaga
     2341 tggtggagca atgcctcggg ggcctgatgg tgagtctggg catcatttac gacctgcaca
     2401 agagcttcc
//
LOCUS       DAOQOC010000398         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26280_length_2409_cov_2.728547, whole genome shotgun sequence.
ACCESSION   DAOQOC010000398 DAOQOC010000000
VERSION     DAOQOC010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26280_length_2409_cov_2.728547"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2220)
                     /locus_tag="PL106_07245"
     CDS             complement(<1..2220)
                     /locus_tag="PL106_07245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNA32903.1"
                     /translation="MIRQATSCVLLLAVQGVRAQSYFQQEVNYRIDVRLDDAKHELHA
                     FEQFDYHNNSPQTLDTIWIHLWPNAYKDRSTALCDQLDRMNNFGLHYATPEQRGFLDS
                     LAFTVGPSSVPVQWGPHPEHIDIAWIKLPSPLAPGEHTTISTPFRVRIPSASFSRLGH
                     AGQAYYITQWYPKPAVLDSEGWHPMPYLTQGEFYSEFGSFDVRITLPANYVVGATGEL
                     QDAHERAWLDSLSTVPYADDQKFKVRKQRARLNATPPSSARTKTLHFKQDRVHDFAWF
                     ADKRFIVRKGSVTLPRSGNTVTTWTMFTPENADIWKDAITYVNESVRLYSSWVGDYPY
                     SACTAIDGGTAAGGGMEYPMITIINDSSDPFELDVVIAHEVGHNWFYGILGSNERDHP
                     WMDEGINSYYEQRYVEARYPGLRIKNVQGLPIGSLSMGEGITYREQNELMYRFNARRN
                     QDSPPDTPSDEFSELDYGTTVYAKSAVAFDNLRGLLGEARYDSCMQAYFGSWRFKHPR
                     PIDLERSFGMDKDPALASTFRDAIMTARKADRRPRHPMRSMVPEIDPRNDSAGFRFPE
                     FKFLAGLERKDRSSIYWTPAIGYNTHDGWMPGITLRNTTVPDQRIEWVLAPLYGTQSH
                     RLAGGGRIAWSHDRLNSGWLRNVHVGVSGFAASQGADATWDYSYQRLVPSLTLDLRSR
                     PNAVQHNIKYRSILLWNRAKRTDETDLIQSSGLAVTLNPFHELSFTSEQTKGIFPFSI
                     "
BASE COUNT          407 a          708 c          782 g          512 t
ORIGIN      
        1 gatgctgaac gggaatatgc ctttggtctg ctccgaggtg aacgagagtt cgtggaacgg
       61 gttgagggtg accgccagac cggacgactg gatcagatcg gtctcgtccg tccgtttggc
      121 cctgttccac aggaggatgg accggtattt tatgttgtgc tgtacggcat tgggccggga
      181 acgcaggtcg agcgtcaggc tcggaacaag ccgttggtag ctgtagtccc acgtggcatc
      241 ggcaccctgc gatgccgcga agcccgacac gcccacatgg acgttgcgca accaaccgct
      301 gttcaggcga tcgtggctcc atgcgatccg cccaccaccg gccaggcggt gactttgtgt
      361 cccgtacagc ggggccagca cccattcgat gcgctggtcg ggcacggtgg tgttgcggag
      421 cgtaatgccc ggcatccatc catcgtgcgt gttgtacccg atagccgggg tccagtagat
      481 gctgctgcgg tccttgcgtt ccagtccggc caggaatttg aattcgggga accggaagcc
      541 agcactgtcg ttgcgtggat cgatctcagg taccatggac cgcatcgggt gccggggccg
      601 tcggtcggcc ttgcgtgccg tcatgatcgc atcccggaac gtgctggcca gagccgggtc
      661 cttgtccatg ccgaatgatc gctccagatc aatgggccgt ggatgcttga agcgccagct
      721 cccgaagtac gcctgcatgc acgaatcgta acgggcttcg ccgagaaggc ccctcaggtt
      781 gtcgaaggcc acggccgatt tcgcgtacac cgtggtgccg taatccagtt cgctgaactc
      841 gtcggacggc gtgtcaggtg gggagtcctg gttccggcgg gcgttgaagc ggtacatcaa
      901 ttcgttctgc tcccggtagg tgatgccctc gcccatgctc aagctgccga tcggcagacc
      961 ctggacattc ttgatacgta accccgggta tcgcgcttcc acgtagcgtt gctcgtaata
     1021 actgttgatg ccctcgtcca tccatggatg atcgcgctcg ttgctgccga ggatgccgta
     1081 gaaccaattg tgccccacct cgtgggcgat caccacgtcc agctcgaacg ggtcgctgct
     1141 gtcgttgatg atggtgatca tcgggtattc catgccgcca cccgctgcgg tgccgccatc
     1201 gatcgccgtg catgcgctgt acggatagtc gcccacccag gagctgtaca gccgcacgct
     1261 ctcgttcacg taggtgatcg cgtccttcca gatgtccgca ttctcgggtg tgaacatggt
     1321 ccacgtggtc actgtgttcc cggaacgtgg cagcgtgacg gagcccttgc gcacgatgaa
     1381 gcgcttgtcg gcgaaccagg cgaagtcgtg tacgcgatcc tgtttgaagt gcagggtctt
     1441 ggtgcgcgcg cttgatggtg gggtggcgtt cagccgtgcg cgctgcttgc gcactttgaa
     1501 cttctggtcg tccgcgtacg gcaccgtgga cagggaatcg agccaggccc gctcgtgcgc
     1561 gtcctgcagc tcgccggtgg caccaacgac ataattcgcc ggcagggtga tgcgcacatc
     1621 aaaactgccg aattcgctgt agaactcccc ttgcgtgagg tagggcatcg ggtgccatcc
     1681 ttcgctgtcg agcacggccg gtttagggta ccactgcgtg atgtagtacg cctgcccggc
     1741 gtggcccagg cgagaaaagg aggcgctggg gatccgtaca cggaagggcg tgctgatggt
     1801 cgtgtgctcc cccggtgcga gtggggacgg taatttgatc caggcgatgt cgatgtgctc
     1861 ggggtgcggg ccccactgca ccggcacgga tgacggtcca actgtgaaag cgagactgtc
     1921 caagaagcca cgttgctcag gtgtggcata atgcagtccg aaattgttca tccggtccaa
     1981 ctggtcgcag agcgcggtgc tgcggtcctt gtaggcgttc ggccagaggt ggatccagat
     2041 ggtatcgagg gtctgagggg agttgttgtg atagtcgaac tgttcgaagg cgtgcagttc
     2101 gtgtttcgca tcgtccaggc ggacatcgat ccggtaattg acctcctgct ggaaatagga
     2161 ctgggcccga acgccttgca cggcgagcag cagcacgcac gaggttgcct gcctgatcat
     2221 agcgcgcagg ttcgttgatg ccatcgcacg aggtccgccg tcggatcgtg gtccgatctc
     2281 ctgtggttcc acacgtcgca tcccgggtcc ggcaagcttc tacttgagca cttcggccag
     2341 cttctcctcc agcgccggac cgcgcaggtt cttggcgagc accttgccgt cccggtccac
     2401 cagcaccgt
//
LOCUS       DAOQOC010000399         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26292_length_2408_cov_3.386740, whole genome shotgun sequence.
ACCESSION   DAOQOC010000399 DAOQOC010000000
VERSION     DAOQOC010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26292_length_2408_cov_3.386740"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PL106_07250"
     CDS             complement(<1..1357)
                     /locus_tag="PL106_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNA32904.1"
                     /translation="MRTLFRKTLVPAYGLARQALTIGALLLIGLVDGQVVQTFNASGT
                     YTVPAGVTRVVVECYGGGGRGGSRSSNGQGGGGGGGAYARSILTVVPGSDHTVTVGAG
                     SSSGTSPGGDSWFSTATTVMAKGGASVPNNNSTGATGGSAAASIGTVKFNGGNGGGVG
                     SSTGGGGGASGGYGGPGGNGAVGVGGTAPPGGGAGGAGGILGAAGTAGTAPGGGGGGA
                     AKWFLSGAAAGGNGATGRVVVSYYTPGSCMTTTTGINGIADNQCAANAHTSVPMVISG
                     QPTILGTAPGNARLVSVGLIISHTYNSDMDITLTSPAGTTRNMVLDRYGSGDNLGNPA
                     TCPSAIFTLQDGGTPLSNLNTSNVTGTFAPEESLAGFTGDPNGMWTLNVCDDAGGDLG
                     VIRYFQLEFCTVPEITATSSNSPICAGSSLTLDATATGSPAPTYSWTGTGTFSPDNTS
                     PN"
     gene            complement(2002..2085)
                     /locus_tag="PL106_07255"
     tRNA            complement(2002..2085)
                     /locus_tag="PL106_07255"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2049..2051),aa:Leu,seq:gag)
BASE COUNT          439 a          757 c          704 g          508 t
ORIGIN      
        1 cgttcgggct cgtattgtcc ggactgaacg tgccggtacc ggtccaggag tacgtgggcg
       61 ccggactgcc cgtcgcggtc gcgtccagcg tcagcgaact gcccgcgcag atcgggctgt
      121 tgctgctcgt tgctgtgatc tccggaacgg tgcagaattc caattggaaa taccggatca
      181 ccccgagatc accgccagcg tcatcacaga cgttcagcgt ccacatgccg ttcgggtcgc
      241 cggtgaatcc ggcgaggctc tcttccgggg cgaaggtgcc cgtaacgttg ctggtattca
      301 ggtttgaaag aggcgttcct ccgtcctgca gggtgaatat tgccgaagga catgttgcag
      361 ggttgcccag gttgtcgcca ctgccatagc gatcgagcac catgttgcgc gtggtccctg
      421 ccggtgaggt cagggttata tccatgtcgc tgttgtaggt gtgggagatg atcaacccta
      481 cgctcactaa ccgtgcgttg ccgggtgctg tgcccaagat ggtcggttgg ccactgatca
      541 ccatgggcac actcgtgtgt gcgttcgccg cacattggtt gtcggcaata ccgttgatcc
      601 cggtcgttgt tgtcatgcac gaacccgggg tgtaatagga tacgacgaca cggcctgtag
      661 cgccgttgcc accggcggcc gcaccagaca agaaccattt cgcggctccg ccaccgccac
      721 cgccaggtgc ggtcccggct gttcccgcag cccccaggat accaccagcg ccgccggccc
      781 caccgccagg gggcgcggta cctccgacgc ccacagcgcc atttccacct ggtccaccat
      841 agccgccgga cgctccccca cctccaccag tggatgagcc gacacctcct ccattccctc
      901 cattgaattt taccgtgcca atggacgctg cggcgctgcc gcccgttgca cccgtggagt
      961 tgttgttcgg tacgctcgca ccgccctttg ccatcacggt ggttgctgta ctgaaccagc
     1021 tatccccgcc gggacttgtc ccggaactgc ttcccgctcc aacggtaacc gtgtgatcag
     1081 aacccggcac caccgtcagg atggatcgtg cgtatgcacc gccgcccccg ccgccgcctt
     1141 ggccgttgga ggatcgcgac ccgccgcgac ctccccctcc gtaacattcc accacgaccc
     1201 gggtgacccc ggccggtacg gtataggtgc ccgaggcatt gaaggtctgt acgacctgtc
     1261 catcgaccag accgatcaat aatagggccc cgatcgtaag ggcctgcctg gccaggccgt
     1321 aagctggcac caaggttttg cgaaacagtg ttctcatggg agtttatggt tgaagttggc
     1381 gttcttgttg cttttggaag atggacctgt caacggtggc cggctttcag cggatcgttg
     1441 gtttttactc cgggaagggt gaacgacgtc gaccatccgg tcgtcggacc agacccatga
     1501 acaagagcct gatgttcaca cacgattaca aaattcaagc gtattttatc gaggttcaat
     1561 gggcctttgt cgatagtttt caacaattaa tcagtaaaat atccgaggag gtgtctcaag
     1621 cgagcatcgc accaatgtgg gggtcgatag ggtgcccaag accggctggc cgctgacggc
     1681 cggaccaggt ttggccgtgg agtggattgt cccggtccgc ttcgcggcct tggggctttt
     1741 ccccgcccat cctcgctcaa gcttcacttg gctcggctga tcgtgaaaaa gccccgccgc
     1801 ttcgcgacag ggcttgatca ccaagtgccc aggactggat tggccgctga cggccggacc
     1861 aggtttggcc gtggagtgga tcaagccggg tgctgctccg cagccctggg gctttttact
     1921 gatcgtcttc gctcaagcaa gcttggctcg ccactcagta aaaagccccg ccgcattcgc
     1981 ggcagggctt tgatcatcaa gtgcccagga ctggactcga accagcacag cctttcggcc
     2041 actaccccct caaagtagcg tgtctaccaa tttcaccacc tgggcaggtg cgttccaagg
     2101 ggcggcaaat atagccgttg ccatctaccg ggaattcgca gcttgttgat gcgcaccaaa
     2161 ggcccagggc acacctcgcc gtgcctagcc ccaccgtaca ggatcagccc atcgaaggcc
     2221 tgctggcgat tggcaatggg catcctgtac aggggaaggc gtcgctcatg taccggcaca
     2281 tggcggttga gtgacgtttc ggcgggctca gcgtgacagt tcattgggag tgttcacgaa
     2341 aagtgttcct ggacactctt gtccccactg tgacgctgag cccgccgaag cgccatactc
     2401 ctgtttac
//
LOCUS       DAOQOC010000400         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26308_length_2407_cov_3.095238, whole genome shotgun sequence.
ACCESSION   DAOQOC010000400 DAOQOC010000000
VERSION     DAOQOC010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26308_length_2407_cov_3.095238"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="PL106_07260"
     CDS             complement(<1..2363)
                     /locus_tag="PL106_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32905.1"
                     /translation="MIGGSPTGPAGSTVLWVPASGLDDASGANPQATPDSTTLYVVQV
                     ADSGCTATDTVLITVQIPTVDAGADVSACDGDTAQLNATGIGLFSWSPSDGLSATDIA
                     DPQAAPGATTTYQVTLTDSLGCTATDSVVVTVNSFGLNINLSPATTSGCQNESMTFTA
                     LPDSSGFTYSWNASGGISVSGINGNSALVTWTGIGAGELTVQANDPNGCSQTIITPFN
                     TLAVPDVNAGPDTTICAGESVQLHGTGNGTLQWSPITGLSDPNIADPIANPELTQTYT
                     LTVTGGNLCVNTDQVTVTVNVLPNANAGPDLQLCLGDSIQLQASGPGTYNWTPDSTLS
                     DANDTNPWAFPTTTTTYTLTLSDSTACSNVDEVTVSVTTPPDPGTGGSASLCGSGVPF
                     TLLDSLGGTPEPGGTWTAPDNSTHGPVFDPAADLAGTWTYTLPGIGACPDASAPLQVT
                     VIVPNAQVTGDNSICVGDTTQLTNTGNVAWTWSPGSGISDTSIPDPFFFPESTTLYTL
                     TATDANGCTATATVEVSVNAPPTADAGTDAAICADNSVTIGGSPTGPSGSTFAWSPDT
                     GLSSATAANPIASPQANTTYTVLVTDGNGCSMIDSVSISVNPLPTVDAGSDVSACTGG
                     SVQLNATGTGSFSWSPPDGLSATDIADPVASPTVATTYTVTVTDGNGCSASDDITVAV
                     AALPTVDAGPDLYLCPGYSVQLQGNGSGTPTWSPGGTVSAPDVFDPEATPLTNTTYTL
                     TITDGNGCTATDETTVTVSDEPPVDAGADQSTCAGQPVVLGGAPTTSV"
BASE COUNT          351 a          750 c          820 g          486 t
ORIGIN      
        1 accgatgtgg tgggggcgcc accaagtacc accggctgcc ctgcgcatgt gctctggtcc
       61 gcaccggcgt ccacgggcgg ttcatcactc acggtcacgg tcgtttcatc ggtcgccgtg
      121 catccattgc cgtcggtgat cgtcagtgtg tatgtggtgt tcgtcaaggg cgtggcttcc
      181 ggatcgaaga cgtcaggagc gcttaccgtg cctcccggcg accaggtggg tgtgccactg
      241 ccgttcccct gcaactgcac gctgtatccg ggacacaggt agaggtcggg gccggcatca
      301 acggtcggaa gcgcggccac ggccaccgtg atgtcatcgg atgccgagca gccgttgcca
      361 tcggtcaccg ttaccgtata cgtggtagct acggtggggc tcgccaccgg atcggcgatg
      421 tccgttgcgc tcaggccgtc gggtggtgac cagctgaagc ttcccgtacc ggtggcgttc
      481 agctgtacac tcccgccggt acaagcggac acatcggagc ccgcatccac ggtcggcagg
      541 gggttcacgc tgatgctcac actgtcgatc atggaacagc cgttgccgtc ggtcaccagc
      601 acggtgtagg tcgtgttcgc ctgaggtgaa gcgatcgggt tcgcggccgt cgcgctgctc
      661 aacccggtgt caggcgacca tgcgaaggtg cttcccgacg ggcctgtcgg gctgcccccg
      721 atcgtcacgg agttgtccgc acagatcgcc gcatcggttc ccgcatcggc agtgggcggg
      781 gcgttcacgc tcacttccac cgtggccgtc gctgtgcagc cgttcgcatc cgtcgccgtc
      841 agcgtgtaca gcgtggtact ctccgggaaa aagaacgggt cggggatcga ggtgtccgag
      901 atgcccgatc ccggcgacca ggtccaggcc acgttgcccg tgttggtcag ttgggtggta
      961 tcaccgacgc agatgctgtt gtcgccggtg acctgtgcgt tgggaacgat cactgtcact
     1021 tggagcgggg ctgaggcgtc aggacaggca ccgataccag gaagcgtgta ggtccacgtg
     1081 cccgccaggt ccgctgccgg gtcgaacacg ggtccgtgcg tgctgttgtc cggtgcggtc
     1141 caggtgccgc cgggttccgg cgttccgccc agcgagtcga ggagtgtgaa gggaaccccg
     1201 ctgccgcaca ggctcgccga tcccccggtt ccgggatcgg gcggcgtcgt caccgatacg
     1261 gtgacctcgt cgacgttgct gcacgccgtg ctgtcgctca aagtgagcgt gtacgtggtt
     1321 gtcgtggtcg ggaacgccca cgggttcgtg tcgttggcgt cgctcaaggt gctgtccggt
     1381 gtccagttgt acgtgcccgg gccactggct tgcagttgga tcgaatcgcc aaggcagagc
     1441 tgcaggtcgg gccccgcgtt ggcgttgggc agcacgttca ccgttacggt cacctggtcc
     1501 gtgttcacgc acaggttgcc tccggtcacg gtgagcgtgt aggtctgggt cagttccgga
     1561 ttcgcgatgg ggtccgctat gttcgggtcg ctcagtccgg tgatcggcga ccattgcaag
     1621 gtgccgttgc cggtaccgtg caactggacg ctctctcccg cgcatatcgt ggtgtcgggt
     1681 ccggcgttca catcaggtac cgccagtgtg ttgaatgggg tgatgatggt ctgcgaacaa
     1741 ccgttcgggt cgttggcctg aacggtgagt tcaccagcac cgatgccggt ccacgtcacc
     1801 agcgcgctgt tgccgttgat gccgctgacg ctgatgccgc cgctcgcatt ccagctgtac
     1861 gtgaagccac tgctgtccgg aagcgcggtg aaggtcatcg actcattctg acaaccgctc
     1921 gtggtcgcgg gggagaggtt gatgttcaac ccgaagctgt tcaccgtcac caccaccgag
     1981 tcggtcgccg tgcagcccag gctgtcggta agggtcacct ggtacgtcgt ggtggcgccc
     2041 ggtgcggcct gcggatcggc gatgtccgtg gcgctgagcc catcgctggg cgaccagctg
     2101 aacaggccga tccccgttgc gttcagctgg gcggtatccc catcgcaagc gctcacatcc
     2161 gcgcccgcgt ccaccgttgg gatctgtacg gtgatcagca ccgtgtccgt ggccgtacat
     2221 ccggaatcgg ccacttgtac cacatacagg gtagtgctgt cgggggtggc ttgcgggttc
     2281 gcaccgctcg catcgtccag accgctggcc gggacccaga ggacggtgct tcccgcaggt
     2341 cctgtggggc tgccaccgat caccgttccc gcgccgggac agattgttgc atccagaccg
     2401 gcttcag
//
LOCUS       DAOQOC010000401         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26357_length_2404_cov_4.282248, whole genome shotgun sequence.
ACCESSION   DAOQOC010000401 DAOQOC010000000
VERSION     DAOQOC010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26357_length_2404_cov_4.282248"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PL106_07265"
     CDS             complement(<1..947)
                     /locus_tag="PL106_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNA32906.1"
                     /translation="MTMPGEHDPVRPAAGRLFLVSGLAALWCGALFGVVGSWQHVLPG
                     VVDAVPFVKSRPLHVSLVVAWIFMGAVGGVYHYLPWITGRPLYSQRMAWWHLVIFLGT
                     GLLVIVSYLMGRFGGREYWEFPPVLGIPIALGWILLVYNFFRTVQKRSGPWPAYLWMW
                     AVGIVFFLLTFTEAHLYLFPGFSGNAVRDLTVQWKAYGALTGSWNMLVYGTSMVVMER
                     MGGDRSVALSRTAFLLFALGFTNLLFGWAHHIYPVPAAPWLRILAYFVSMTEWIILAR
                     MIRQWRGTLNAGMEQRHSLTHLFLFASEVWVFLNLFLALL"
     gene            complement(931..1377)
                     /locus_tag="PL106_07270"
     CDS             complement(931..1377)
                     /locus_tag="PL106_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNA32907.1"
                     /translation="MAAFVLTARHRRTILVGLVLLFIVQSAITYTTATSVKDERAMYN
                     EAASRGAQLYRDLNCTACHQLYGLGGYMGPDLTNTMGTPGKGPAYAKAFILHGTQRMP
                     ALGVTPEQADDIVAFLQAVDATGTHPIRHIDLTPWGTYRSMHDDAR"
     gene            complement(1491..2030)
                     /locus_tag="PL106_07275"
     CDS             complement(1491..2030)
                     /locus_tag="PL106_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNA32908.1"
                     /translation="MRPTAILGLLLMACGNPAPAPEQGTPTTSTPSAGQSAVSDDLSE
                     RNVLQVAIGSPDHKTLVAAVQHVKYEDVLVNAGPFTVYAPTDAAFNALPAGTLDDLLK
                     PENKAKLQDILEYHVALGVLPPEKMSDGRSVGMANGGNVKFGKTGDGAVMINDAKVLG
                     SVPASNGLVVVIDKVLLPQ"
     gene            2194..>2404
                     /locus_tag="PL106_07280"
     CDS             2194..>2404
                     /locus_tag="PL106_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32909.1"
                     /translation="MRGSSTDQLRPDHRLLVLLLVGKEHGLHVIGRKAAFRLSATPVE
                     EEGNAIGALDQRSFFRKTATLRHVDN"
BASE COUNT          489 a          705 c          783 g          427 t
ORIGIN      
        1 agcagggcca ggaagaggtt gaggaagacc cacacctcgc tggcgaacag gaagagatgc
       61 gtgagcgaat gacgttgttc catcccggcg ttgagcgttc cgcgccactg cctgatcatg
      121 cgtgcgagga tgatccattc ggtcatgctc acgaagtagg cgaggatgcg gagccagggg
      181 gcggcgggta ccggatagat gtgatgcgcc caaccgaaga gcaggttggt gaatcccagg
      241 gcgaaaagca ggaaagccgt gcgcgacagg gccaccgacc ggtcaccacc catgcgctcc
      301 atcaccacca tgctcgtgcc gtaaacaagc atgttccacg agcccgtgag tgcgccgtaa
      361 gccttccact gcacggtgag gtcgcgtacc gcattgccgc tgaacccggg gaacaggtat
      421 agatgtgctt cggtgaaggt gagcaggaag aatacgatgc cgacggccca catccacagg
      481 tacgcgggcc atggcccgct gcgcttctga accgtacgga agaagttgta aacgagcagg
      541 atccacccca gcgctatggg tatgcccagc acaggcggga attcccagta ttcccggcca
      601 ccgaatcggc ccatcaggta cgatacgatg acgagtaacc ccgtgccgag gaagatgacg
      661 aggtgccacc aggccatacg ctgcgaatac aagggcctgc ctgtgatcca gggcaggtaa
      721 tggtacacgc cgcccaccgc tcccatgaag atccaggcca ccaccagcga gacgtgcagc
      781 gggcgcgact tcacgaaggg aacggcatcc actacgccgg gcagcacgtg ctgccatgag
      841 ccgaccaccc cgaagagtgc tccgcaccac aacgcggcaa ggcccgacac caggaacagg
      901 cgtcccgctg cagggcggac ggggtcgtgc tcaccgggca tcgtcatgca tggatcggta
      961 ggtgccccat ggcgtcaggt cgatgtgacg gatgggatgc gttccggtgg cgtccacggc
     1021 ttgcaggaac gcgacgatgt cgtcagcctg ttccggcgta acgccaagtg cgggcatgcg
     1081 ttgcgtgccg tggagaatga aagccttcgc gtaggccggc cccttgcccg gggtgcccat
     1141 cgtgttggtg aggtcggggc ccatatagcc gcccagcccg tacaactggt ggcatgccgt
     1201 gcagttgaga tcccggtata attgagcccc cctcgaagcg gcctcgttgt acatggcacg
     1261 ttcatccttt accgatgtgg cagtagtgta tgtgatggcc gattgcacga tgaacaacag
     1321 caccaagccg acgaggatcg tgcggcggtg acgtgcggtg aggacgaatg cggccatcag
     1381 cgagcacggc gaatgttcac caacaggtac ggcaagggcg cccttgcgga cgccccggcc
     1441 ggcttttcac cattccgcca gcggcccggt gggagaggcg catggcgggg ttattggggc
     1501 agcaggacct tgtcgatcac caccaccagg ccgttgctgg ccggcaccga gccgagcacc
     1561 ttcgcatcgt tgatcatcac cgcgccatcc ccggtcttgc cgaacttcac attaccgccg
     1621 ttggccatgc ccacgctgcg gccatcgctc attttctccg gaggcagcac gcccaaggcc
     1681 acatggtatt cgaggatatc ctgcaatttg gccttgttct ccggcttcaa cagatcatcc
     1741 agcgtaccgg cgggcaaggc gttgaacgcg gcatcggtcg gagcatacac ggtgaacgga
     1801 ccggcgttca ccagcacgtc ctcgtatttc acatgctgca cggctgctac cagtgttttg
     1861 tgatcaggcg agccaatggc cacctgtaga acgttccgtt ccgacaggtc gtcgctcacg
     1921 gcgctttgtc cggcgctcgg ggtggatgtg gtcggggtgc cctgttccgg cgcaggtgcg
     1981 gggttgccgc aggccatgag caggaggccg aggatggcag tgggacgcat gaggcaggtt
     2041 gtggttttca tggttgtttg tccaacgcca cgaaattcac cgcaggtgac catcccggtc
     2101 atgacgggcg tcaataaaag tgaaatttgt cgcggcctgt gctggatagg gtgatggtcg
     2161 tggtggggtg aaggacagga agcaatgtgt ggaatgcgcg gaagttcgac ggaccaattg
     2221 cggcccgatc atcgattgct ggttcttctg ctggtgggaa aggaacacgg cctccatgtg
     2281 atcgggcgta aggcggcctt ccggttgtca gcgacccccg ttgaagagga gggcaacgcg
     2341 atcggcgccc tggatcaaag atccttcttc cggaaaacgg caacgctgcg ccacgtggac
     2401 aatg
//
LOCUS       DAOQOC010000402         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26381_length_2403_cov_3.985520, whole genome shotgun sequence.
ACCESSION   DAOQOC010000402 DAOQOC010000000
VERSION     DAOQOC010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26381_length_2403_cov_3.985520"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PL106_07285"
     CDS             complement(<1..921)
                     /locus_tag="PL106_07285"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNA32910.1"
                     /translation="MKKMDINRSGRSLVLWLTLLTVPAFAGNPDRAGSAGATQLLINP
                     WARSAGMGLANSARLRGVEGMFGNIAGLARVRKTEIYFTSSRWLEGSGVKIQSGAIGQ
                     KIGESGVLGISFTSINYGEVQVTTVDQPDGGIGSFRPTSGNIAVAYSKEFSNSIYGGV
                     LVRVASESISNVRATGFSFDAGIHYVTGPTDNVHFGIALKNVGPAMRFSGDGMAVQGL
                     LTQGSDQLTIEQRSDRFELPSQMNIGAAYDWNINEMNRLTVAGNFSSNSFTKDQFTLG
                     LEYGFRKLVYLRGGYTYEKDITDDKLRQTVF"
     gene            complement(940..>2403)
                     /locus_tag="PL106_07290"
     CDS             complement(940..>2403)
                     /locus_tag="PL106_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32911.1"
                     /translation="ADRSIEVANEQLIVDWGISVTIDQPEYTGQFSAYTKPLGAGVEY
                     ADAGAAGAGWYMGVPDVDGETAWNWIRSGLAGDTALDFPDYTGYDDDQTYEKTFGTWS
                     PFRLVGDASFQPGASSVAAARADSYMSLAPSIQVTFTSDKSKWTRCAVLEAGDDNSLP
                     GVDVDKLRIKPDPSRDKNGRKSGDDGYNDAEGTLNGAQPTGMSWFPGYAIDLETGERL
                     NVAFAENTFWGLNGIGADMIWNPITTPDNFASPEILMAQNHWTYFFTNRRRSFNDVSQ
                     MPLYDQGAYLYTRLSSGTGADRTRVWRSCAWVGSAVANMLLSPEQGLVPADLRIRLDI
                     SKAYLPYIPYEGAPDTPISTDRNGGLGLYTFSTDGLRTETEVNSVEREHLDRINVVPN
                     PYYAFSGYETSRLDNRVKFINLPRTCTISIYSVSGTLVRQFKKDNDLTFVDWDLKNHK
                     NVPVSGGTYICHVNVPGSGEKVLKWFGVMRPVDLQNF"
BASE COUNT          429 a          740 c          740 g          494 t
ORIGIN      
        1 gaagaccgtc tggcgcagct tgtcgtcggt gatgtccttc tcgtaggtgt aaccgccacg
       61 caggtacacc agcttgcgga agccgtattc cagtcccaag gtgaattggt ccttggtgaa
      121 ggagttgctg gagaaattgc ctgccacggt caaccggttc atctcgttga tgttccagtc
      181 gtaggcggca ccgatgttca tctgcgaggg cagctcgaag cgatcgctgc gttgctcgat
      241 ggtgagctgg tcgctgccct gggtgagcag gccctgcacg gccataccat cgccgctgaa
      301 gcgcatggcg gggcccacgt tcttgagggc gatcccgaag tggacgttgt ccgttggtcc
      361 ggtcacgtaa tggataccgg catcgaagga gaatcccgtg gcgcgcacgt tgctgatgct
      421 ctcgctggcc acacgcacga ggacaccgcc atagatgctg ttggagaact ccttggagta
      481 ggccacggcg atgttgccgc tggtggggcg gaacgagccg ataccaccat cgggctggtc
      541 cacggtggtc acctgtacct ctccgtagtt gatgctggtg aaactgatgc ccagtacacc
      601 gctctcaccg atcttctggc cgatggcccc ggattggatc ttcacaccac tgccttcgag
      661 ccagcgcgaa ctggtgaagt agatctccgt cttgcgcacg cgggccagcc cggcgatgtt
      721 gccgaacatg ccttccacgc cccgcagccg tgcactgttg gccaggccca tgccagcgct
      781 gcgcgcccaa gggttgatca gcaactgggt ggctccggcc gaaccggcac gatcagggtt
      841 gcccgcgaac gccggtaccg tcagcaaggt gagccagaga acgagcgatc gcccgctccg
      901 gttgatatcc atcttcttca tgctccttcg ttcttgggat cagaaattct gcaggtcaac
      961 ggggcgcatc acaccgaacc acttgagcac cttctcgccc gagcccggca cgttcacatg
     1021 gcagatgtac gtccctccgg aaaccggcac gttcttgtgg ttcttcaggt cccagtccac
     1081 gaaggtgagg tcgttgtcct tcttgaactg ccgcaccagc gtaccgctca cgctgtagat
     1141 gctgatggtg caggtgcggg gcaggttgat gaacttgacc cggttgtcca gtcggctcgt
     1201 ttcgtacccg ctgaacgcgt agtacgggtt gggcaccacg ttgatgcggt ccaggtgttc
     1261 gcgctccacg ctgttcacct cggtctcggt gcgcaggcca tcggtactga aggtgtacag
     1321 gcccagacca ccgttgcgat ccgtggaaat gggtgtgtcg ggggcccctt cgtaagggat
     1381 gtacggcaag tacgccttgc tgatgtccaa gcggatgcgc agatccgcag gtaccagtcc
     1441 ctgctcgggg ctcaacagca tattggccac ggctgaacct acccaagcgc aggaacgcca
     1501 tacacgggtg cggtcggcac cggtaccact gctcaggcgc gtatagaggt atgcgccctg
     1561 gtcgtacagc ggcatctgcg atacatcgtt gaagctgcga cggcggttgg tgaagaagta
     1621 ggtccagtgg ttctgggcca tgaggatctc cggtgaggcg aaattgtcgg gtgtggtgat
     1681 cgggttccag atcatgtccg caccgatccc gttcaggccc cagaacgtgt tctcggcgaa
     1741 agccacgttc aaccgttcac ccgtttccag atcgatcgcg tagcccggga accagctcat
     1801 tccggtgggc tgcgccccgt tcaaggtgcc ttcggcgtcg ttgtacccat catcgccgct
     1861 cttccggcca ttcttgtccc ggctgggatc gggcttgatg cgcagcttgt ccacgtcaac
     1921 accgggcagg gaattgtcat cgccggcttc gagcaccgca caacgcgtcc acttgctctt
     1981 gtcggaggtg aaggtcacct ggatactggg cgccagggac atatacgaat cggcgcgcgc
     2041 ggccgccacg gaagaggcac cgggctggaa gctcgcatcg cccaccaggc ggaacggcga
     2101 ccaggtcccg aaggtcttct cgtaggtctg gtcatcgtcg tagccggtat agtcggggaa
     2161 gtccaaggcg gtatcgccgg ccagacccga acggatccag ttccatgcgg tctcgccgtc
     2221 cacatccggg acccccatgt accaacctgc gcccgctgcc cctgcgtcgg catactccac
     2281 tcccgcgccc agcggcttcg tataggcgct gaattgaccg gtgtattcgg gttgatcaat
     2341 ggtgacggag ataccccaat cgacgatcaa ctgctcattg gccacctcga tgctccgatc
     2401 ggc
//
LOCUS       DAOQOC010000403         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26384_length_2403_cov_3.321976, whole genome shotgun sequence.
ACCESSION   DAOQOC010000403 DAOQOC010000000
VERSION     DAOQOC010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26384_length_2403_cov_3.321976"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PL106_07295"
     CDS             complement(<1..1087)
                     /locus_tag="PL106_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNA32912.1"
                     /translation="MTKPTSAKASVGNRTIKKVAVLGSGVMGSRIACHFANIGAEVLL
                     LDIVPKEAAESKKPAERNKIVNDALAFALKSNPSPIYDKRFAKRISTGNFDDDLPKIK
                     DCDWVIEVVVERLDIKQQVLANVEKHRKPGTLITTNTSGIPIHAIAGGRSADFRKHFC
                     GTHFFNPPRYLPLLELIPGPDTDPAVIAFLEEYGQRILGKVTVLAKDTPAFVANRIGV
                     FGIMDVFNLVIDMGLSVEEVDRLTGPVIGHPKSATFRTADVVGLDTLVKVAQGVKDNC
                     PNDERNSLFAIPPYLQQMVEKNMLGSKTGKGFFFKDRSSAKGEILSLDLKTLEYKPQV
                     KPKFATLDAAKKEDNLAKRLKLVYNGTD"
     gene            complement(1199..1630)
                     /locus_tag="PL106_07300"
     CDS             complement(1199..1630)
                     /locus_tag="PL106_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNA32913.1"
                     /translation="MRPEETIDFPIRRVWSRISRLYNTEAAKYGGSMAIGQILLNIEP
                     EGTPSTKLGPRMGMEARSLVRTLQTMEEEGLIKRVAATDDKRVVRIHLTAKGKQMRDV
                     SRDTVVKFNQAVQARFTQAQLNNFRQVMSELDRILEQEQLF"
     gene            complement(1745..>2403)
                     /locus_tag="PL106_07305"
     CDS             complement(1745..>2403)
                     /locus_tag="PL106_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32914.1"
                     /translation="GGTLTSSTNGPCVTVEWNASGIGTVEVTSDVGDCVFSSEQTISI
                     VDPPQASFLVEMDTTCTGIKARAVNSSTAGTTAQWLFNGQPLTPGDTTELILPFSLNS
                     TVQLTVTDVNGCTDQDAQSFSVAPYSEVVEFRIPNVFSPNGDGKNDRFELITSQHLSE
                     CSEIRIFDRWGKEVFASGGGRMAWNGSRGGGEKASEGVYYYIVKVDQESFHGHVSLMR
                     "
BASE COUNT          388 a          706 c          749 g          560 t
ORIGIN      
        1 tgtcggtgcc gttgtacacc agcttcaggc gcttggccag gttgtcctct ttcttggccg
       61 catccagcgt ggcgaacttg ggtttcacct ggggcttgta ttccagcgtc ttcaggtcca
      121 gtgagaggat ctcgcccttc gcgctgctgc ggtccttgaa gaagaagccc ttgccggtct
      181 tgctgcccag catgttcttc tccaccatct gttgcaggta aggcgggatg gcgaagaggc
      241 tgttgcgctc gtcattcggg cagttgtcct tcacgccctg ggccactttc accagggtat
      301 ccaggcccac cacgtcggcg gtgcggaagg tggcgctctt cgggtggccg atcaccgggc
      361 cggtgaggcg gtccacttct tccacgctca ggcccatgtc gatcaccagg ttgaacacgt
      421 ccatgatgcc gaacacgccg atgcggttgg ccacgaaggc gggcgtgtcc ttggccagta
      481 cggtgacctt gcccaggatg cgctgtccgt actcttcgag gaaggcgatc acggccggat
      541 ccgtatccgg gccgggaatc agctccagca gcggcaggta gcgcggtggg ttgaagaagt
      601 gcgtgccgca gaagtgtttg cggaagtcgg cgctgcggcc tccggcgatc gcatggatcg
      661 ggatcccact ggtgttggtg gtgatgagcg tacctggttt acgatgcttc tccacgttgg
      721 ccagtacctg ctgcttgatg tcgagccgct ccaccaccac ttcgatcacc cagtcgcagt
      781 ccttgatctt cggcaggtcg tcgtcgaagt tgccggtgct gatgcgcttg gcgaagcgct
      841 tgtcgtagat gggggagggg ttgctcttga gcgcgaaggc cagtgcatcg ttgacgatct
      901 tgttgcgctc ggcgggcttc ttgctctcag cggcttcctt gggtacgatg tccaatagca
      961 gcacttccgc gccgatgttg gcgaagtggc acgcgatgcg gctgcccatg acgccggagc
     1021 cgagcacggc gactttcttg atggttcggt tcccgaccga agccttggcg gaggttggtt
     1081 tggtcatgtg tgtaggaatg cttcaccaca gaggcacaga gagcacggag gaatgcgatc
     1141 gatgttggac tccgcttgca tttggttttc tctgtgtcct ctgtgcctct gtggtgaact
     1201 agaagagttg ttcctgttcg agtatccgat caagttccga catcacctgc cggaagttgt
     1261 tgagctgagc ttgggtgaag cgcgcttgca cggcctggtt gaactttacc acggtatcgc
     1321 ggctcacgtc gcgcatctgt ttgccctttg cggtgaggtg gatccgcacc acgcgcttgt
     1381 cgtccgtggc cgctacccgc ttgatcaatc cctcctcctc catggtctgc aaggtgcgca
     1441 cgaggctgcg tgcctccatg cccatgcgcg gaccgagctt ggtgctgggc gtgccttccg
     1501 gttcgatatt gagcaggatc tgtccgatag ccatgctgcc tccgtacttg gccgcttcgg
     1561 tgttgtacag gcgcgagatg cggctccaca cgcggcgaat ggggaagtcg atggtctctt
     1621 cggggcgcat gggcgggtgc ggacgaaagg taatgcctga cgttatgcac gcataacaaa
     1681 tgggctgacc gaatttgaac aggaggaata ccagcgcgga cccctggttg atcccttggc
     1741 gcactcagcg catcaggctg acatggccgt ggaacgattc ctggtccacc tttacgatgt
     1801 agtagtagac cccttcgctc gctttctcac ctccgccccg actcccattc cacgccatgc
     1861 ggccaccccc gctggcgaac acctccttgc cccagcgatc gaagatccgg atctcggagc
     1921 attcgctcaa gtgctggctg gtgatgagtt cgaagcggtc gttcttacca tccccgttcg
     1981 gtgagaagac gttcgggatc ctgaactcga ccacttccga atagggggcc acactgaagg
     2041 attgcgcatc ctggtccgtg catccgttca catcggttac ggtgagttgc accgtgctgt
     2101 tcaagctgaa cggcaggatg agttcggtcg tgtcgcccgg ggtgagcggc tgcccgttga
     2161 acagccactg ggccgtggtg ccagccgtcg aactgttcac cgcccgggct tttattcctg
     2221 tacacgtggt atccatttcc accaggaacg aggcctgtgg cggatccacg atgctgatgg
     2281 tctgttcact gctgaagaca caatcgccca catcgctggt gacttccacg gtgccgatgc
     2341 cgctggcgtt ccactccacc gtgacgcacg gcccgttggt cgatgaggtc agcgttcctc
     2401 cgg
//
LOCUS       DAOQOC010000404         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26459_length_2400_cov_2.730917, whole genome shotgun sequence.
ACCESSION   DAOQOC010000404 DAOQOC010000000
VERSION     DAOQOC010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26459_length_2400_cov_2.730917"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="PL106_07310"
     CDS             complement(<1..2110)
                     /locus_tag="PL106_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNA32915.1"
                     /translation="MTRTFPWSLLLPGLIITSCGTNPESDPHAQGQNGAHGPLLSAVP
                     ATTSGITFSNTITESPDLNYFTYVYAYNGAGVAVGDIDNDGLADIYFTGNQASDRLYR
                     NLGGMTFEDITEKAIGPKAKEGWRTGVTMADVNGDGRLDIHVCRSGFTTDPALLGNLL
                     YVNNGDGTFTERAKEFGIADTSHSTQAAFFDADLDGDLDLHVINHPMGRMKGLTNFTA
                     LRAVQEGRAPTDRFYRNEGGHFTDRTAEARMGNFDYTLGQSVSDLDQDGWPDVYTAND
                     FDAPDQMYLNQHDGSFAEQSQQRLRHISNFGMGCDVADHNNDGYPDIITLDMTADEHV
                     RNKTNMGSMDQHKFWALVNGGFFYQYMLNTLQLNNGNGTWSEIGQLAGVARTDWSWAP
                     LFCDLDNDGWKDLLVTNGFLHDVRNNDYARSVYDTIRSGAGYYGSLGLVPSTKLRNYL
                     FRNNAAEASGLTFADSSQAWGFTTPHNSQGAAYADLDNDGDLDLVINNMNDTASLYTN
                     NATNAFADRHWLRVRALNGATDAFGAKVSLRAGGITQYQELYPSRGYQSCVEPVIHFG
                     LGPATNIDTLEVRWPGGAYTLLTNVKVDQLLTVKREEALASGPSPRSGHGPLLTEQAA
                     ALGLNFRHVENPYDDFELERLLPHKQSELGPTLSTGDANGDGLDDLFTGSGHGQTPKL
                     FVQRTDGTFVAVPGPWERYAG"
     gene            complement(2174..>2400)
                     /locus_tag="PL106_07315"
     CDS             complement(2174..>2400)
                     /locus_tag="PL106_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32916.1"
                     /translation="KANAVYLNMDRNHPNQEFYATIWDYNGPNFHYDPVTYLLNRKVC
                     ITGKITLYNDIPRISVNNENEIELWDEIVK"
BASE COUNT          383 a          687 c          801 g          529 t
ORIGIN      
        1 ggcccgcata acgctcccat ggaccgggta ccgcaacgaa ggtgccgtcc gtccgctgca
       61 cgaagagctt cggcgtctgt ccgtggccac tgccggtgaa gaggtcgtcc agcccgtccc
      121 cgttggcgtc cccggtggag agggtgggtc cgagttcact ctgcttgtgc gggagcagac
      181 gctccagttc gaagtcgtca tacgggttct cgacatgcct gaagttgagg ccgagggcgg
      241 cggcctgttc cgtcaggagc ggtccatgcc cactgcgggg cgaggggcct gaagccaggg
      301 cttcctcgcg cttcacggtc aggagctggt ccaccttcac gttggtgagc agggtgtacg
      361 cgccacctgg ccatcgcact tccagggtat caatattggt cgcggggccg aggccgaaat
      421 ggatcacggg ttccacgcag ctctgatacc cgcgcgatgg ataaagttcc tgatattgtg
      481 tgatgccacc agcacgcagg gacaccttcg ccccgaaggc atcggtagcg ccgttcaagg
      541 ccctcacgcg gagccagtgc cggtcggcga aggcgttggt ggcgttgttc gtgtagagtg
      601 aggccgtgtc gttcatgttg ttgatcacca gatcgagatc gccatcgttg tccaggtcgg
      661 cgtacgcggc tccctggctg ttgtgcggcg tggtgaagcc ccatgcctgc gagctgtcgg
      721 cgaaggtgag cccggaggct tccgcggcat tgttgcggaa gaggtagttg cgcaatttgg
      781 tactgggcac cagtccgagg gatccgtagt agccggcccc tgagcggatc gtatcgtaga
      841 cgctgcgggc gtagtcgttg ttgcgcacgt cgtgcaggaa gccattggtc accagcaggt
      901 ccttccagcc atcattgtcc aggtcgcaga acagcggtgc ccagctccag tcggtgcggg
      961 ccaccccggc gagctgtccg atctcgctcc aggtgccatt gccgttgttg agctggaggg
     1021 tgttcaacat gtactggtag aaaaagccac cgttgaccag ggcccagaac ttgtgctggt
     1081 ccatgctgcc catgttggtc ttgttgcgca cgtgctcatc ggccgtcatg tccagcgtga
     1141 tgatgtcggg atacccgtcg ttgttgtggt cggccacgtc gcagcccatg ccgaaattgc
     1201 tgatgtggcg caggcgttgc tggctctgct ccgcgaagga accgtcgtgc tggttcaggt
     1261 acatctggtc cggcgcgtcg aaatcattgg ccgtgtacac gtcgggccag ccatcctggt
     1321 ccagatcgct cacgctctgc cccagcgtat agtcgaagtt gcccatgcgc gcttcggccg
     1381 tacggtcggt gaaatggcca ccttcgttcc ggtagaagcg gtcggtgggt gcgcggccct
     1441 cctgcaccgc gcgcaatgcg gtgaagttgg tgaggccctt catgcggccc atggggtggt
     1501 tgatcacgtg caggtcgagg tcgccgtcca gatcggcatc gaagaaggcg gcttgtgtgc
     1561 tgtgcgacgt gtcggcgatc ccgaactcct tcgcgcgctc ggtgaaggtg ccgtcgccgt
     1621 tgttcacgta cagcaggttg ccgagcagtg cgggatcggt ggtgaagccg ctgcgacaga
     1681 cgtggatgtc gagacgcccg tccccgttca cgtcggccat ggtcacgccg gtcctccagc
     1741 cttccttcgc cttgggtccg atggcctttt cggtgatgtc ctcgaaggtc attccaccga
     1801 gattccggta gaggcgatcg ctcgcctggt tccccgtgaa gtagatatcg gcgagtccat
     1861 cgttatcgat gtccccgacc gccacaccgg caccgttgta cgcatacacg tacgtgaagt
     1921 agttcaggtc ggggctttcc gtgatggtat tgctgaaggt gatcccgctc gttgtggccg
     1981 ggaccgcact gagcaggggc ccgtgtgcgc cgttctggcc ctgtgcatgg ggatcgctct
     2041 cagggttcgt gccgcaggaa gtgatgatca ggccaggcag gagcaaagac caggggaagg
     2101 tgcgcgtcat ggcattgaag tgaagtggag ctcaggtccg gaaccggctg aaccggttat
     2161 tgcgaggcgt gttctatttg acgatctcat cccacagctc gatctcgttc tcattgttca
     2221 cgctgatccg cggaatgtcg ttgtacaggg tgatcttccc cgtgatgcac accttcctgt
     2281 tcagcaggta ggtcacgggg tcgtagtgga aattgggtcc gttgtagtcc cagatggtgg
     2341 cgtagaactc ctggttcggg tggttccggt ccatgttgag gtacacggcg ttggccttct
//
LOCUS       DAOQOC010000405         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26520_length_2397_cov_2.411187, whole genome shotgun sequence.
ACCESSION   DAOQOC010000405 DAOQOC010000000
VERSION     DAOQOC010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26520_length_2397_cov_2.411187"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PL106_07320"
     CDS             complement(<1..408)
                     /locus_tag="PL106_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32917.1"
                     /translation="MPLHFSFAPYRLLFKRPFATAHGLRNGTDALFIRAEEDGVVGHG
                     EVTLPPYVKETIAGSIERLNALARKSFPRPGQLLTQLDTLDEIKNAPAFRAGVSTALI
                     DLISNHRQWTVRKLIEVTGEEEPITLMTLGICPA"
     gene            complement(533..1549)
                     /locus_tag="PL106_07325"
     CDS             complement(533..1549)
                     /locus_tag="PL106_07325"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA32918.1"
                     /translation="MRALKTILIILGSLIALWLVLSLMGPKDSHLERSTVIKASPNTI
                     FEYIRFLKNQDEWGVWYKMDPNAKYELVGEDGTVGAKRTWDGQVVRQGAQTIVALEQD
                     KSVKADLEFGPMLGHGSLDLTPQGDSTKVSWSMRMEIPFIFRAAALFMANDEGARDFE
                     GGLANLKTICEAKQVEMDKASVPTFAIAVSEWPAKLYVGKREVVKWDDMKAFFTKHFG
                     ENMATVTKVGVQPAGSPSGVFFEWSEKDRTADLIAGIPVPLDARSKLKGSDLYEMPAS
                     KVLSLDYYGGYSGTAGAHMAMDKYIQDNHLTHHTNVVEEYITDPGQEPDSTKWLTRIT
                     YLVK"
     gene            1724..2299
                     /locus_tag="PL106_07330"
     CDS             1724..2299
                     /locus_tag="PL106_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32919.1"
                     /translation="MLLRTSIGLLATSLLLALPGCKGGAKTTTSAGPKVEMPFTGNKY
                     ESNNRFFRGTGTGVSVKQNIARSKSDLEAKQQLAAQVGTNVRAVTDQYLGQTENAQAA
                     DVADKFQSLVREVMDTQIGDLRKIGEEVRQNQTTQEYTVYTAYEIKKNAMFRFLKKQA
                     QTNQKLDDAARKRIEEILDEEIKKADLEDRD"
BASE COUNT          459 a          711 c          743 g          484 t
ORIGIN      
        1 cgccggacag atccccaggg tcatcaaggt gatcggctcc tcctcgccag ttacttcgat
       61 caacttgcgc acagtccatt ggcggtggtt gcttatcaga tcgatcaaag cggttgatac
      121 ccccgcccgg aacgccgggg cattcttgat ctcatccagt gtatccaact gggtgagcaa
      181 ctggcctggc cgcgggaaag acttgcgtgc aagggcgttg agccgctcga tcgaacctgc
      241 gatggtttct ttcacgtagg gcggaagggt cacctcgccg tggccgacaa ccccgtcctc
      301 ctcggcccgg atgaagaggg cgtcggtacc gttgcgcagg ccatgcgccg tggcgaaggg
      361 tcgtttgaac aggagcctgt acggggcgaa gctgaagtgg agcggcatgg agccacgaag
      421 gtgacgaaac cgcgcggcaa ttccatggga cgggatcatt atttttcgac gggccgcatc
      481 aatggaaagc ccggcacggt gaccgggctc catttcgtgg ggatgatccg gtctacttca
      541 ccaagtatgt gatgcgtgtg agccacttgg tgctgtcggg ctcctgtccg ggatcggtga
      601 tgtactcctc gaccacattg gtgtggtgcg tgaggtggtt gtcctggatg tacttgtcca
      661 tggccatgtg tgcgcccgcc gtaccgctgt agccgccgta gtagtccaaa ctgagcacct
      721 tgctggccgg catctcgtac agatcggatc ctttcagctt ggacctcgca tccaacggca
      781 cggggatccc ggcgatcagg tcggccgtgc ggtccttttc actccactcg aagaagacgc
      841 cactgggcga tccagcgggt tgcacaccga ccttggtcac cgttgccatg ttctcgccga
      901 aatgcttggt gaagaaggct ttcatatcgt cccacttgac cacttcgcgc ttgccgacat
      961 acagtttggc gggccattca gacaccgcga tcgcgaaggt tggcaccgag gctttgtcca
     1021 tttccacctg tttcgcctcg cagatggtct tgaggttggc caggccgcct tcgaaatcac
     1081 gagcgccctc gtcgttggcc atgaacaggg cggccgcccg gaagatgaag gggatctcca
     1141 tgcgcatgga ccagcttacc ttggtgctgt caccttgtgg tgtcaggtcc agcgagccat
     1201 ggccgagcat cggcccgaac tccagatccg ccttcacgct cttgtcctgt tcaagcgcaa
     1261 cgatggtctg ggcgccctgc cgcaccactt gcccgtccca tgttcgtttg gcccccacgg
     1321 taccgtcctc gcccaccaat tcgtacttgg cgttcgggtc cattttgtac catacgcccc
     1381 actcgtcctg gttcttcagg aatcggatat actcgaaaat ggtgttcggc gaggccttga
     1441 tcaccgtgct ccgctcaagg tgggagtcct tcggacccat caggctgagc accagccaca
     1501 gtgcgatcaa tgaaccgagg atgatgagga tggttttgag tgcgcgcatg gtggatacgc
     1561 ccgatgcccg gcgtgcggct tttggtatgt ggaggtgaaa agctgcgaaa ggtggtgctt
     1621 cgccgggatc tttccaaccg gggttggtgc cagcaacgcc ggtccgtggc atggtgtttt
     1681 ctttgcccca tccaccgatc aaccggtatc aacacgaccc gccatgctgc tccgtacctc
     1741 cattggcctg cttgccacca gcctgctcct cgccttgccg ggctgcaagg gcggagccaa
     1801 gaccacaacc tcggccggtc cgaaggtgga aatgcctttc acgggcaaca agtacgagag
     1861 caacaaccgc ttcttccggg gcacgggcac gggcgtgagc gtgaagcaga acattgcacg
     1921 gagcaagagc gacctggagg cgaagcagca gctggcggcc caggtgggca ccaacgtccg
     1981 cgcggtaacc gaccagtacc tggggcagac cgagaacgcc caggccgccg acgtggcgga
     2041 caaattccag agccttgtgc gcgaggtgat ggacacccag atcggcgacc tgcgcaagat
     2101 cggtgaggag gtgcgccaga accagaccac ccaggagtac acggtgtaca ccgcttacga
     2161 gatcaagaag aacgcgatgt tccgcttcct gaagaagcag gcccagacga accagaagct
     2221 ggatgacgcc gcccggaagc gcatcgagga gatcctggac gaggagatca agaaggctga
     2281 cctggaggat cgcgattgag tgccggggtc ggattgtcgg ttgtccgttg tcggttgtcc
     2341 gttctgccag gttgaccgaa gaacgcttcg gccccgcgtt tgttaagagg gcagcag
//
LOCUS       DAOQOC010000406         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26561_length_2395_cov_2.757692, whole genome shotgun sequence.
ACCESSION   DAOQOC010000406 DAOQOC010000000
VERSION     DAOQOC010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26561_length_2395_cov_2.757692"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PL106_07335"
     CDS             <1..588
                     /locus_tag="PL106_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA32920.1"
                     /translation="GDERFVLIGNFCTDQLTRNEKMPEGERGAYYFIDDVNIRLAPQG
                     AKTSPLPGICEPPPPKPRVVDHVSSKQVDILHVEPEVGKHIRLDNIQFDFDKAELKPE
                     SKDEVETVLDLLTDYPLMRIEIGAHTDDQGADDYNLKLSEARAKAVAYWLIKEKVDAQ
                     RITWKGYGETMPLAKGITEVDRATNRRVEFKVVER"
     gene            complement(754..1008)
                     /locus_tag="PL106_07340"
     CDS             complement(754..1008)
                     /locus_tag="PL106_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNA32921.1"
                     /translation="MNITFNELRNIKDSLPDGSIHRIARELDLNVETVWNYFGGYSHP
                     GGRPMGVHLEGGANGGVVALDDTRILDMARRILDEVPHHA"
     gene            1117..>2395
                     /locus_tag="PL106_07345"
     CDS             1117..>2395
                     /locus_tag="PL106_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA32922.1"
                     /translation="MLFLRTIALPCLCLLVLRAMAQQYDIRVFGVEDGLPGSTVTAIA
                     QDSTGFLWVRTDGGDCRFDGRNFRDVPLGSSFPIPTPLAMPPTAIPGVDDGLRTTCTL
                     VSPRTNRYWQGTETGVQVFPQRKAPEPLFKLYWGNGLGSNDITCLFEDSSGVMWIGTR
                     QGGLNRYISDAVLQFGREGGSYAKVMSMYRSAAGDLRIMAGENGIAYAHLPAGITGSL
                     EGLWPGGTIRAIMGPGKKQLYAGNDEGIFHLEGFGSPHENPSIGPFIYQEAPVFAALT
                     DARGSSWAATGKGLITIAPTSEWLHVGQDTLAACALAFHGDSILIGTHQGLYAAPLDN
                     SAANWTPIFHAGKEDITCLAVDRADNVWIGTADAGVVRYRNGTTQRWTKESGLASDRI
                     RQLLLDAYQNLWVGTDRGFHLFELDELQEQVIDI"
BASE COUNT          490 a          759 c          715 g          431 t
ORIGIN      
        1 ggcgatgaac gtttcgtgct catcggcaac ttctgcaccg accaactcac acgcaatgag
       61 aagatgcccg aaggcgagcg tggcgcctac tatttcattg atgacgtgaa catccgcctg
      121 gcgccgcagg gtgcgaaaac atcaccgctc ccgggtatct gcgaaccgcc cccgcccaag
      181 ccccgcgtgg tcgatcacgt gagcagcaag caggtcgaca ttctccatgt ggagccggaa
      241 gtgggcaagc acatccggct ggacaacatc cagttcgact tcgacaaggc ggaattgaaa
      301 ccggaaagca aggatgaagt cgaaaccgtg ctcgacctcc tcaccgacta cccgctcatg
      361 cgcatcgaga tcggggcaca tacggacgat cagggagccg acgattacaa cctgaagctg
      421 agcgaggccc gcgccaaggc cgtggcctac tggttgatca aggagaaagt ggacgcccag
      481 cggatcactt ggaaaggtta tggtgaaacg atgcccttgg ccaaggggat caccgaagtg
      541 gaccgggcca ccaaccggcg agtggagttc aaagtggtgg agcgctgaac ggcattttcg
      601 ccccaccttc caacgtacat accgtgcgcg atacggaccg tcccacagcc cgtcggctgt
      661 tcgtcaactc acatgtatac ccgaccccat caaagggata agcctcatcc accgccctgg
      721 cgcgggttcc cgttcgggcg gtatccttga gcgctacgcg tgatgcggaa cctcatccag
      781 gatccggcgc gccatgtcca ggatccgtgt gtcgtccaac gcaaccacgc caccattcgc
      841 accgccttcc aaatgcacac ccatgggcct accgcccgga tgtgagtacc caccgaagta
      901 gttccagacc gtttccacgt tcaggtccag ttcccgcgca atgcggtgga tgctgccatc
      961 gggcaggctg tccttgatgt tccggagctc gttgaaagtg atgttcatgg ccgcttggtt
     1021 ttggagacgc ccgaagttaa tgcttatcct gttgatcggt tcgcccggtg gaaaaaaagg
     1081 agaagttcgt gcggccgtcg atcacgatca ccctgcatgc tcttcctccg cacgatcgcc
     1141 ctgccatgcc tgtgtctgct cgtcctgcga gccatggcgc agcaatacga catccgtgtg
     1201 ttcggggtgg aggacggcct gcccggcagc acggtaacag ccatcgcgca ggacagcacc
     1261 gggttcctgt gggtgcggac cgatggcggg gactgccgat tcgatggccg gaatttcagg
     1321 gacgtgccat tgggctcgag tttcccgatc cccacaccgc ttgccatgcc gccaaccgca
     1381 atacccggtg tcgacgatgg actccgtacc acctgcacat tggtatcgcc acgaacgaac
     1441 cggtactggc aggggaccga aaccggcgta caggtctttc ctcagcggaa ggccccggaa
     1501 ccgctgttca aactttactg ggggaatgga ctgggcagca acgacatcac ctgtcttttc
     1561 gaagacagca gcggtgtgat gtggatcggc acccgtcaag ggggcctgaa ccggtacatc
     1621 agcgacgctg tgctccaatt cggccgggag ggcggttcct atgcgaaggt catgtccatg
     1681 tatcgctccg ctgcaggcga cctgcgcatc atggccggcg agaacggcat cgcttacgcg
     1741 cacctcccgg cgggcatcac agggtcattg gagggcctgt ggcccggcgg caccattcga
     1801 gcgatcatgg gcccgggaaa aaagcaactc tatgccggga acgatgaagg gatcttccac
     1861 ttggaaggtt tcggatcccc gcacgagaac ccctccatcg ggcccttcat ctaccaagaa
     1921 gccccggtct tcgccgcgct caccgatgcc cgtggcagtt cctgggccgc caccggaaaa
     1981 gggctgatca cgatcgcccc gaccagcgag tggttgcacg tggggcagga tacccttgct
     2041 gcgtgtgccc tggcgttcca cggcgactcg atcctgatcg gcacccatca ggggctgtac
     2101 gcagccccct tggacaacag cgccgccaac tggacgccga tcttccatgc cggcaaggag
     2161 gacatcacct gcctcgccgt ggaccgtgcg gacaatgtct ggatcggcac tgcggatgcc
     2221 ggcgtggtgc gctaccggaa cggcaccaca caacgctgga ccaaggagag cggcctggcc
     2281 agcgaccgca tccgccagct cctgctcgat gcgtaccaga acctctgggt gggtacggac
     2341 cgtggtttcc acctgttcga gctggacgag ctgcaggagc aggtcatcga tatca
//
LOCUS       DAOQOC010000407         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26650_length_2392_cov_2.696620, whole genome shotgun sequence.
ACCESSION   DAOQOC010000407 DAOQOC010000000
VERSION     DAOQOC010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26650_length_2392_cov_2.696620"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PL106_07350"
     CDS             <1..1375
                     /locus_tag="PL106_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA32923.1"
                     /translation="AAIVLTSIGQLGSFPLFLTSVALAGAIQLTLGILRAGVIAYYFP
                     NSVIKGMLAGIGLIIILKQIPHAVGDDKDWMGDESYAQVDELNTFQELWFAVQNPAWG
                     AVIITMACIAIMLLWERPFIKRFKALTYLPGPLLAVILGTVLSRLFGGQEGLMLADLH
                     FVRLPDISDGFGTALTFPDPAGLSNGLVWTTAITIAIVASLETLLCVEATDKLDPEKR
                     ITPANRELRAQGVGNILSGLVGGLPITQVIVRSSANMQSGARSKLSAVLHGALILLSL
                     WLIPGLMNMIPLASLAAILIITGFKLAKPPLFTDMYKQGWYIFIPFLATVLGVVFIDL
                     LKGVALGMCVGIFFVLRNSYLTPFHVVDDTDEDGPIRRVILSEEVTFFNKASIQRVLS
                     RLPAGTHLVLDADRTMNLDPDVLEIIHEAQQREQERGVKIELVCHRTVTKRSTEELIE
                     AVVGQRA"
     gene            1401..2033
                     /locus_tag="PL106_07355"
     CDS             1401..2033
                     /locus_tag="PL106_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNA32924.1"
                     /translation="MRTHTRETQEHLTPQKALEILIEGNGRFVNNLKANRDLLKQVNE
                     TSAGQFPFAAVLSCIDSRTSAELIFDQGLGDIFSIRIAGNCINEDILGSMEFACKVAG
                     SKLILVLGHTGCGAVKGACDDVRMGNLTTLLEKIRPAVDDTVTTGERNSSNAAFVQDV
                     ALRNVQIALQRIPQRSPILKEMLDQGRIMLAGAMYDVATGQVGIVKPPVP"
     gene            2108..>2392
                     /locus_tag="PL106_07360"
     CDS             2108..>2392
                     /locus_tag="PL106_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNA32925.1"
                     /translation="MRKENPELFTELAKHQNPFFLWIGCSDSRVPPNQITGTMPGDMF
                     IHRNIANVVVHTDMNLMSVLDYGVNVLRIDHVIVCGHYGCGGVKAAMGPEF"
BASE COUNT          494 a          719 c          742 g          437 t
ORIGIN      
        1 ggccgccatt gttctcacgt ccatcggtca actgggatca ttcccgttgt tcctcacgag
       61 tgtggcgctc gcgggggcca tccaactcac cctgggcatc ctgcgggccg gcgtgatcgc
      121 ctattacttc ccgaacagcg tgatcaaagg catgctggcg ggcatcggtc tgatcatcat
      181 cctcaagcag atcccgcatg ccgtggggga tgacaaggat tggatgggtg acgagagcta
      241 cgcacaggtg gacgagctca ataccttcca ggaactctgg tttgcggtgc agaacccggc
      301 gtggggtgcg gtcatcatca ccatggcctg catcgcgatc atgctgttgt gggagcggcc
      361 gttcatcaag cggttcaaag cgctgacgta cctgcccggg ccgctgctgg cggtgatcct
      421 gggcacggtg ctgtcccggt tgttcggagg gcaggaggga ctcatgctcg cggacctgca
      481 cttcgtgcgg cttccggaca tttccgacgg gttcggtacg gcacttacct tccccgaccc
      541 cgcaggcctg agcaatggtc tggtgtggac cacggccatc accatcgcca tcgtggccag
      601 cttggaaaca ctgctgtgcg tggaagccac ggacaaattg gatcccgaga agcgcatcac
      661 cccggcgaac cgggaattga gggcccaggg agtgggcaat atcttgagcg gccttgtggg
      721 cggtcttccc atcacgcagg tgatcgtgcg cagttcggcc aatatgcaga gcggtgcacg
      781 atcgaagttg agcgcggtcc tccacggagc actgatcctt ctcagcctct ggctgatacc
      841 cgggctcatg aatatgatcc cgctggcgag cctggccgcc atcctgatca tcacgggctt
      901 caagctggcc aaacccccgc ttttcacgga catgtacaag cagggctggt acattttcat
      961 cccgttcctg gccaccgtgc tcggtgtggt gttcatcgac ctgttgaagg gagtggcact
     1021 gggcatgtgc gtagggatct tcttcgtact gcggaacagc tacctcaccc ccttccatgt
     1081 ggtggacgat accgacgagg acggcccgat acgccgggtg atcctgagcg aggaggtcac
     1141 cttcttcaac aaggcgagca tccagcgcgt gctctcccgc ctgcccgccg gcacccacct
     1201 ggtgctggat gccgaccgca cgatgaacct cgatccggat gtgctggaga tcatccacga
     1261 agcccagcag cgcgaacagg agcggggggt gaagatcgaa ctggtctgcc atcggacggt
     1321 cacgaagcgc agtactgaag aactgatcga agcggtggtg ggccaaaggg cctgaatcgt
     1381 gaccaacaga accacttgga atgagaacgc acacccggga aacacaagag cacctgacgc
     1441 cccagaaggc gttggagata ctcatcgagg gcaacggacg tttcgtgaac aacctgaagg
     1501 cgaaccgcga cctgctcaaa caggtgaacg agaccagcgc gggccagttc ccgttcgccg
     1561 ccgtgctcag ttgcatcgac agccgcacca gtgccgaatt gatcttcgat cagggtctgg
     1621 gcgacatctt cagcatccgc atcgcgggca attgcatcaa tgaggacatc ctcggcagca
     1681 tggaattcgc ctgcaaggtg gccggatcga aattgatact ggtccttggc cacacagggt
     1741 gcggagcggt caaaggggca tgcgacgatg tgcgcatggg caacttgacc acgctcctgg
     1801 agaagatccg gccagcggtt gatgatacgg tgaccaccgg cgaacggaac agttcgaacg
     1861 ccgctttcgt gcaggatgtc gccctgcgca acgtacagat cgccctgcag cggatcccgc
     1921 agcgcagccc catcctgaaa gagatgctcg atcaaggccg gatcatgctg gccggtgcca
     1981 tgtacgatgt ggcaacgggg caggtcggga tcgtaaaacc acccgtgcca tgaagcatct
     2041 tcgcgacatg aaccacacgg atctgcacca caccctgctg gccaacaacg cggagtggag
     2101 cgcgcgcatg cgcaaggaga accccgagct cttcaccgaa ctggccaagc accagaaccc
     2161 gttcttccta tggatcggct gcagcgacag tcgggtgccg ccgaaccaga tcaccggcac
     2221 catgccgggc gacatgttca tccaccgcaa catcgccaac gtggtggtgc acacggacat
     2281 gaacctgatg agcgtgctgg actacggggt gaacgtgttg aggatcgacc acgtgatcgt
     2341 gtgcgggcac tacggctgtg gcggggtgaa agccgcgatg ggccccgagt tc
//
LOCUS       DAOQOC010000408         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26708_length_2390_cov_4.135760, whole genome shotgun sequence.
ACCESSION   DAOQOC010000408 DAOQOC010000000
VERSION     DAOQOC010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26708_length_2390_cov_4.135760"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1012)
                     /locus_tag="PL106_07365"
     CDS             complement(131..1012)
                     /locus_tag="PL106_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32926.1"
                     /translation="MERITATACVLLSLTGVSGQMTVGAGTNLHLDAGTSLRVESPMT
                     WTLEAGSTVVNNGTIVLGPSTELSEAPGAAITGSGIERTTRDLDSPLNSNDPGGLGAI
                     LTTSTAPGPTLVERGHLPITAFNGETSIARWIRITPTNNNGLGATLAFRYDVQELGSV
                     PETEQRLHVFDGQWNWWYLPSTVNTGSSTVTTTGLDSLGSFTTFAMDLPTGMSGDNTA
                     GGYALIGPPGEQQFLRVPDGECAGSLDFIAASGAQAANFTPQWTGGLHALPRPALASG
                     LYRLVVNGTRGFPLLVP"
     gene            1133..1531
                     /locus_tag="PL106_07370"
     CDS             1133..1531
                     /locus_tag="PL106_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32927.1"
                     /translation="MSIKNLIADQLGYFSVQDIASTLIGVMAAAVLGYVIALLARAKD
                     PGAKEMAMTAAVMAFASGLVRGSVPLAIALVAAVLLVRGATELTSRAALLRFTALAIG
                     LGCGASASVVVMAVGIPLALLLRWGTAERS"
     gene            1538..>2390
                     /locus_tag="PL106_07375"
     CDS             1538..>2390
                     /locus_tag="PL106_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNA32928.1"
                     /translation="MAPSQFRSLSGLLVALTAALLLAAALPPDKYHSRGGRSDAPVIK
                     PGSTSSGEVELRMDVPPGTRVRYTLDGTDPGPFSTLYEGPFTLDGHTHADRLVVVPTS
                     LQWRHPVGDLPHGVLVKARCSWSDGSAGPVVTRTVLPASHGQLPVFALSVDEDDFFGE
                     ERGIYVTGNAIFGLDKQALLDPPHDHKWWKYPGNFNWRGKGSERRAHLDYFAPGSPID
                     ADPIWSVETGVRINGNNSRGFPQHALRLILDEPLNRPLFGPEHGTGHRRLLLRTAGND
                     MDHAFFRD"
BASE COUNT          377 a          755 c          775 g          483 t
ORIGIN      
        1 ggaagggaca gaccggtcac ctgccgatcc gtactgtccg gcccatcgag tatgatccgt
       61 gcgggcacct cccattgtgc ggcacccgac agggcgaggc tggccgtaag gagtgcgagg
      121 ttctggcgga tcatggtacc agcaatggga acccccgggt cccgttcacg accaaccgat
      181 aaagaccgct ggccagagcc ggtcgcggaa gcgcatgcag ccccccggtc cattgcggtg
      241 tgaagttggc ggcttgcgcg ccactggcgg ctatgaagtc caagctgccc gcacattcac
      301 cgtccggcac gcgcaggaac tgctgctccc cgggtggtcc gatcagcgca tatccgcctg
      361 ccgtgttgtc cccgctcatg ccggtgggca ggtccatggc gaaggtggtg aacgatccca
      421 gactgtcgag cccggtggtg gtgaccgttg atgatcccgt gttcacggta cttggaaggt
      481 accaccagtt ccactgcccg tcgaacacat gcaggcgttg ttccgtttcc ggtacgctgc
      541 ccagttcctg tacatcgtaa cggaatgcca gcgtcgcccc cagtccgttg ttgttggtcg
      601 gtgtgatcct gatccagcgc gcgatcgagg tctcgccgtt gaaggcggtg atcggcaggt
      661 gcccgcgttc caccagcgtt ggaccagggg ccgtgctcgt ggtcaatatg gctcccaatc
      721 cgccggggtc gttgctgttc aacggcgaat ccaggtcccg tgtcgtgcgt tcgatgccgc
      781 tgccggtgat ggcagctccg ggcgcttcgg aaagttcggt gctcgggccc aatacgatgg
      841 tgccgttgtt caccacggtg ctccccgcct ccagggtcca ggtcatcggg ctctccacgc
      901 gcagggaggt gcccgcatcc aggtgcaggt tcgtgcccgc acccacggtc atctggcccg
      961 atacgccggt caacgatagc agaacacagg ctgtggcggt gatgcgttcc atggtggtac
     1021 cggggctaca ggtcttgttc caccgaggga acggacttcg tagcgagccg tgccaaatgt
     1081 aaacgggtgc ccgtaccggc ctacattcgc gggctcctca agcgggccgt acatgagcat
     1141 caagaacctg atcgccgatc aactgggcta cttttccgtg caggatatcg cgagcacctt
     1201 gatcggtgta atggccgctg ccgtgttggg ctacgtgatc gcactgctcg cccgggcgaa
     1261 ggaccccggc gcgaaggaga tggcgatgac cgctgcggtc atggccttcg cctccggctt
     1321 ggtgcgtggc tcggtgccgt tggccatcgc gttggtggcc gctgtgttgc tggtgcgtgg
     1381 tgccaccgaa ttgaccagtc gggccgcact gctccgattc accgccttgg cgatcgggtt
     1441 gggctgtggt gccagtgctt ccgtcgtggt gatggccgtg ggtatcccgc tcgcactcct
     1501 gctgcgttgg ggcaccgccg aacggtcctg atcgcccatg gcgccctcgc agttccgctc
     1561 cctttctggt ctcctcgtcg cgctaacggc tgccctcctg ctggccgcag ccctgcctcc
     1621 cgataagtac cattcgcgtg gtggtcgatc ggacgccccg gtgatcaagc cgggctcaac
     1681 gtcatccggt gaggtggaac tgcgcatgga cgtacctccg ggaacgcggg tgcgatacac
     1741 gttggacggt accgatccag ggcccttttc cacgctctac gaaggcccgt tcacactcga
     1801 tggccatacg cacgccgatc ggcttgtcgt ggtgcccact tccttgcaat ggaggcatcc
     1861 ggtgggcgat cttccgcatg gcgtgctggt gaaagcccgt tgttcgtggt ctgatgggtc
     1921 ggctggaccc gtggtgacac ggacggtgct gcccgcatca catggccaac tgccggtatt
     1981 cgccctctcg gtggatgagg atgacttctt cggggaggag cgtggcatct acgtgacggg
     2041 caacgcgatc ttcgggctgg acaagcaagc gctactggat ccgccccacg atcacaaatg
     2101 gtggaagtac cccggcaact tcaattggcg cggcaaagga tcggagcgac gggcgcacct
     2161 ggactatttc gcgcccggtt ccccgatcga tgctgatccc atctggtccg ttgagacggg
     2221 ggtgcgcatc aacggcaaca actcacgcgg tttcccgcaa catgcgttgc gcctgatcct
     2281 ggatgagccc ttgaataggc ccttgttcgg cccggaacac ggcaccggcc accggcgcct
     2341 gctgctgcgc acggcgggga acgacatgga ccatgcgttc ttccgcgatg
//
LOCUS       DAOQOC010000409         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26710_length_2390_cov_3.488651, whole genome shotgun sequence.
ACCESSION   DAOQOC010000409 DAOQOC010000000
VERSION     DAOQOC010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26710_length_2390_cov_3.488651"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PL106_07380"
     CDS             <1..1473
                     /locus_tag="PL106_07380"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNA32929.1"
                     /translation="DHKLTDVGVQLEMMDGIENVLTRNIEGQQYLPTGVAINNVALSN
                     ELQSYNTLLAQRDQKRRNLGPDHPELQLLDKQIGNLRGSIRDNLTTLRSELRTTLDAN
                     RYQQGLIENRTRSLPRRERMLVEKERRRSVVQDLYLYLLQKREESAINMAVTTSTGMV
                     VEPPGMPDRPVSPKPRQVWLVALFLGLAIPGAIVLLLRRLNDRVEDAGAIRSITSVPV
                     LATIANSRSKESMVVRAGSNSVVAETFRLLRAGLMYLTPGSDLRTLLITSSTSGEGKS
                     FIAMNLGMTLALAGKRIVILELDLRKPKQERYFGLGTTDKGVVSYLVRKDVAVRDIIR
                     NSGHHPGLDVILSGPVPPDPAELILSDRLRGLVAELRESYDTIILDTPPVGLVADALQ
                     LKDLAQATMYVVRVGYTHLPQLRTVEEIRAGEKLPRPFIVLNGMRYDGPGRYGYGYGY
                     GQGHGYFAEHGASGGLAGRMRRWAGGNGRAGAPEHSGARK"
     gene            1537..>2390
                     /locus_tag="PL106_07385"
     CDS             1537..>2390
                     /locus_tag="PL106_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNA32930.1"
                     /translation="MDRSISGMLWVALDKLGASGTNFVITVVLARLLFPEDFGLVAMA
                     MVFLEVSSVFVESGFSTALIREKEISQADRSTAFLFNVTAAVLLYAALFACAPLIAGL
                     YKVPALVPVIRVMGLGLIIDAATIVPSSALVQRVDMRARTLARLIAATLSGGIGVLLA
                     WRGHGAWALVARMLVNSALLAVLLHRFDPWRPTFSFHRGSFKRLFGFGSRILATGLLD
                     KFFSQAYRLVIGKVYAAATLGLYTQAGGFVSMAVNTLFRPVQTVAYPVLSKMRDDPSR
                     LKDGHRLM"
BASE COUNT          415 a          763 c          790 g          422 t
ORIGIN      
        1 gaccacaagc tcaccgatgt gggcgtgcaa ctggagatga tggacggcat cgagaacgtg
       61 ctcacacgca acatcgaggg gcagcaatac ctgccaacgg gggtggccat caacaacgtg
      121 gcgctgagca atgagctgca gagctacaac acgctactgg cgcagcggga tcagaaacgg
      181 cgcaacctgg gtcccgacca ccccgagttg caattgctgg acaagcagat cggcaacctg
      241 cgcgggtcga tacgggacaa cctgaccacg ttgcggtccg aactgcggac cacactggac
      301 gccaaccgct accagcaggg cctgatcgag aaccgcacgc gtagcctgcc ccggcgcgag
      361 cggatgctgg tggagaagga acgtcggcgg agcgtggtgc aggacctgta cctctatctt
      421 ttgcagaaac gcgaggagag cgccatcaac atggccgtta ccacgagtac gggcatggtg
      481 gtggaaccgc ccggcatgcc cgaccggccg gtgagcccga aaccgcggca ggtgtggctg
      541 gtggccctct tcctcggcct cgcgatcccc ggtgccatcg tgcttcttct ccgtcgtctg
      601 aacgaccgcg tggaggacgc gggcgcgatc cgctccatca cgtccgtgcc ggtgctggcc
      661 accatcgcca acagcaggtc aaaagagagc atggtggtgc gtgccggatc gaactcggtg
      721 gtggccgaaa cgttccgcct gttgcgcgcc ggcctgatgt acctgacccc gggcagcgac
      781 ctgcgcaccc tgctgatcac ctccagcaca tcgggcgagg gcaagtcatt catcgccatg
      841 aacctcggga tgacgttggc gctggcgggc aagcgcatcg tgatcctcga actggacctg
      901 cgcaagccca agcaggagcg ctacttcggt ctcggtacca cggacaaggg cgtggtgagc
      961 tacctggtac gcaaggacgt ggccgtgcgg gacatcatcc gcaacagcgg gcaccatccg
     1021 gggctggacg tgatcctgag cgggcccgta ccgccggacc cggcagaatt gatcctctcg
     1081 gatcggttgc gggggctcgt tgccgaactg cgggagagct atgacaccat cattctggac
     1141 accccgccgg tgggtctggt ggccgatgcg ttgcaactga aggacctggc ccaggccacc
     1201 atgtacgtgg tgcgcgtcgg ctacacgcac ctgccgcagt tgcgcacggt ggaagagata
     1261 cgtgctggcg agaaactgcc ccggccattc atcgtgctca acgggatgcg gtacgatggc
     1321 ccgggaaggt acggctatgg ctacggctac ggccagggcc atgggtactt cgcggagcat
     1381 ggcgcatccg gtggccttgc tggccgtatg cggcggtggg ccggcggcaa cggccgtgcc
     1441 ggcgcacccg agcacagcgg tgcccggaag tgagtggcga tgaccctgga aagccattcc
     1501 ccaaaacaag gtagcgatca cgcgggtcct tcgctgatgg accgctcgat atcgggcatg
     1561 ctctgggtgg ctctggacaa actgggcgcc agcggcacca acttcgtcat caccgtggtg
     1621 ctggcacggc tgctgttccc ggaggacttc ggcctggtgg ccatggcgat ggtcttcctg
     1681 gaggtatcgt cggtattcgt ggagagcggc ttctccaccg ccctcatccg cgagaaagag
     1741 atcagccagg ccgaccggtc cactgcattc ctgttcaacg ttaccgcggc ggtcctgctc
     1801 tatgccgcac tgttcgcctg tgccccgctg atcgccgggt tgtacaaggt cccggccctc
     1861 gttccggtca tccgcgtgat ggggctcggc ctgatcatcg acgcggccac gatcgttccc
     1921 tcatcggcac ttgtacagcg ggtggacatg cgggcgcgta ccctcgcccg gctcatcgcg
     1981 gccaccctgt ccggtggcat cggcgtgctg ctggcctggc gagggcacgg cgcgtgggcc
     2041 ctggtggcgc gcatgctggt gaacagcgcg ctgctcgccg tgctcttgca ccgcttcgat
     2101 ccgtggcggc cgaccttcag cttccatcgg ggatcgttca agcgcctctt cggcttcgga
     2161 tcgcggatcc tggccaccgg cctgctggac aagttcttct cgcaggccta ccgccttgtg
     2221 atcggcaagg tgtacgcggc agcgaccctc ggcctgtaca cccaggcggg cggattcgtc
     2281 agcatggccg tgaatacgct cttccgtccc gtgcagacag tagcttatcc ggtgctgtcg
     2341 aaaatgcgcg acgatccatc gcggctgaag gatggccatc gcctgatgtt
//
LOCUS       DAOQOC010000410         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26712_length_2390_cov_3.233833, whole genome shotgun sequence.
ACCESSION   DAOQOC010000410 DAOQOC010000000
VERSION     DAOQOC010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26712_length_2390_cov_3.233833"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PL106_07390"
     CDS             complement(<1..584)
                     /locus_tag="PL106_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNA32931.1"
                     /translation="MSPILLLSIVFGYFLLLLGIAWYTGRGAGEHAFYSGDRKSLWWV
                     VAFGMIGTSLSGVTFISVPGAVQKGHWQYLQLVIGFAIGYWIVAGVLLPLYYRMGLTS
                     IYGYLRERLGMISYRTGASWFILSRLIGATLRIYVVLNVIHIFTLEGMGIPFWLTAAV
                     VMLMIVLYTFQGGVKTIVWTDTLQTLFMLAAMIGT"
     gene            760..>2390
                     /locus_tag="PL106_07395"
     CDS             760..>2390
                     /locus_tag="PL106_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA32932.1"
                     /translation="MSNSSFRVFLTTLHIPCLMTTALAQGVMIHDASTGAMLDIAFSD
                     IRVDVQDQVALVTSTQRFMNNTADTLTVKYAYPLASTASATRVRWMRTDSIWHTASMV
                     AQPQDTVLPGTGGGLPMDPDLQDYLGASPLYFNVSDPVPPGTWISVELTYVQLLPYAN
                     ARVELIASSDYAQVMTGVLPMMDIDVSVRSQRALLGIDISGTGTWSPTPDMPFVSTDS
                     AHFHVHDTGIPASCGFAIGYDLDPLAYGITCLSNYLPDSLVKCDELANGFFVLLIEPE
                     PTSQVVQKDFVIVIDKSGSMTGTKIQEAKDAASFMVNHLNAGDAFNVIAFDSFNTPWT
                     SALQPFNAANMQAALNWIASIQAGGGTNINDAITEGIEDFSTSPAGHARSIVFLTDGQ
                     DQSSNEVILSNAHALRMSIAPDLQLFTFGIGEGFNEQLLNQLAVQNNGVSQFLETANF
                     SQVMSDFYTQIQNPVLLSPTASFDHPDVQHLYPDPLLGLFVGQQMVIVGRYAVPGLVN
                     LHLSGFASGSPVSFDFQFDLTGTYDPARLFIPKIWA"
BASE COUNT          490 a          765 c          688 g          447 t
ORIGIN      
        1 gtgccgatca tggcggccag catgaagagc gtctgcagcg tatcggtcca gacgatggtc
       61 ttcacccctc cctgaaaggt gtacagcacg atcatcagca tcaccacggc ggcagtgagc
      121 caaaagggga tgcccattcc ctccagggtg aagatatgga tcacgttcaa cacgacgtag
      181 atgcgcagcg tggccccgat gaggcgtgaa aggatgaacc aggaggcacc ggtgcggtag
      241 ctgatcatgc ccaggcgctc gcgcaggtag ccgtagatgc tagtgaggcc catgcggtag
      301 tacaacggca agagcacacc ggccacgatc cagtagccga tggcgaagcc gatcaccaac
      361 tgcaggtact gccagtggcc tttctgcacg gcgccgggta cgctgatgaa ggtgaccccg
      421 ctgaggctgg tgccgatcat gccgaaggcc accacccacc agaggctctt gcggtcgccg
      481 ctgtagaacg cgtgctcgcc cgccccgcgc cccgtgtacc acgcgatgcc caggagcaac
      541 aggaaatagc cgaagacgat ggaaaggagc aggatggggc tcatggtgcg caaagcaaaa
      601 cgggcgtacg catcaaaccg ccccttcgcg caccgagttc tccttcatcc gttgtgagcg
      661 aagcttttcc gatccgtcag gcagcggatc ggcgaggtgc cctgtccttc atccgtgaga
      721 ccggtttgtg cgttcggacc acccttcaga tccaccacca tgtcgaactc cagtttccgg
      781 gtatttctta ccacgctcca cattccttgc ctgatgacca ccgcattggc ccagggcgta
      841 atgatccacg atgcgagcac cggtgcgatg ctcgacatcg cgttcagcga catccgcgtt
      901 gatgtgcagg accaggtggc attggtcacc agtacccagc gcttcatgaa caacacagcg
      961 gatacgctga cggtgaagta cgcctacccg ctggcctcca ccgcgagtgc cacacgtgtg
     1021 cgatggatgc gcaccgacag tatctggcat accgcctcga tggtggctca gccacaggac
     1081 acggtgctgc ccggcaccgg aggtggacta ccgatggatc cggacctcca agactacctc
     1141 ggcgcctcgc cactgtactt caacgtgagc gaccctgttc cgcccggcac atggatcagc
     1201 gtggaactga cctacgtgca actcctgccc tacgcgaacg cacgggtgga gctgatcgct
     1261 tcaagtgact acgcccaagt gatgacaggg gtgttgccca tgatggacat cgatgtctcc
     1321 gtgcgatcgc agcgtgccct gctcggcatt gacatcagcg gcaccggcac ctggtcgccc
     1381 acccccgaca tgcccttcgt tagcaccgac tccgcgcact tccatgtaca cgacacgggg
     1441 ataccggcca gttgcggctt cgccatcggt tacgacctgg atccgttggc gtacgggatc
     1501 acctgccttt ccaactacct gccggattcg ctcgtcaaat gcgacgaact ggcgaacggc
     1561 ttcttcgttc tgctgatcga gccggagccg acgagccagg tggtgcaaaa ggacttcgtg
     1621 atcgtgatcg acaagtccgg cagcatgacg ggcaccaaga tccaggaagc gaaggacgcc
     1681 gcatcgttca tggtgaacca tctgaacgcc ggtgacgctt tcaatgtgat cgcgttcgac
     1741 agtttcaaca ccccgtggac cagtgccctc cagccattca acgcggcgaa catgcaggcc
     1801 gctttgaact ggatcgcgag catacaggcc gggggcggca ccaacatcaa cgatgccatc
     1861 accgagggca ttgaggactt ctccacctcc ccggccggtc atgcacggtc gatcgtgttc
     1921 ctcaccgacg gacaggacca gtccagcaat gaggtgatcc tttcgaatgc gcacgctctg
     1981 cggatgtcca tcgctccgga cctgcagctg ttcaccttcg gtatcgggga aggcttcaac
     2041 gagcagttgc tgaaccagct ggccgtgcag aacaacggcg tgtcgcaatt cctggagacc
     2101 gcgaacttct cgcaggtgat gagcgacttc tacacccaga tccagaaccc cgtgttgttg
     2161 agcccgacgg ccagcttcga ccatccggac gtgcagcacc tgtatcccga tccgttgttg
     2221 ggcctgttcg tgggccaaca gatggtgatc gttggccgct acgccgttcc cggcctcgtc
     2281 aacctgcact tgagcggctt cgcttcaggc agtccggtat cattcgactt ccagttcgac
     2341 ctcacgggaa catacgatcc ggcccggctg ttcatcccga agatctgggc
//
LOCUS       DAOQOC010000411         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26726_length_2389_cov_3.640960, whole genome shotgun sequence.
ACCESSION   DAOQOC010000411 DAOQOC010000000
VERSION     DAOQOC010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26726_length_2389_cov_3.640960"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PL106_07400"
     CDS             complement(<1..176)
                     /locus_tag="PL106_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA32933.1"
                     /translation="MKNLFSSWRSDVPSSVVVFLVAMPLCLGIALASGAPLIAGLIAG
                     IIGGIVVGLYSGSA"
     gene            complement(272..595)
                     /locus_tag="PL106_07405"
     CDS             complement(272..595)
                     /locus_tag="PL106_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32934.1"
                     /translation="MNFSHVQQRGPGRTVWSMPMRVLSLVMSAYLFVLPQMLTSQRVV
                     AIFEDCGSQPPPIIEEEVLKHAVPFEEHVRIPDPEPLMTARFLQFEEEMLDHPVLEVP
                     DQPPR"
     gene            complement(675..1061)
                     /gene="apaG"
                     /locus_tag="PL106_07410"
     CDS             complement(675..1061)
                     /gene="apaG"
                     /locus_tag="PL106_07410"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNA32935.1"
                     /translation="MATQVTHDIRVSVKATYSEEQSDPKVGRFLFSYRIRIENRSGRT
                     VQLLRRHWHIVDSLQGPREIKGPGVVGETPVLPPGEQFTYSSFCDLRSGFGRMNGSYR
                     MRHTDDDSTFDVTIPAFDLIYPIAGN"
     gene            1150..1749
                     /locus_tag="PL106_07415"
     CDS             1150..1749
                     /locus_tag="PL106_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32936.1"
                     /translation="MFRSPSCVLLLALATATFAQRPTATLVNPGLMRLDLEGNRLFAG
                     LPDPSIGVDDIALFPVEPASPVAGGRTFMPSMEGELLDRRTERLGEKTAIFVHAVPTP
                     YGDTAHYSYLVSWNDRRIWFSGLSTDPTALLAATDLDLVFLSPAMARAIEATGKRMNT
                     RMMMLYPVPEGYDRINATGPCDYCKVLAPAVGESIQLFR"
     gene            complement(1777..>2389)
                     /locus_tag="PL106_07420"
     CDS             complement(1777..>2389)
                     /locus_tag="PL106_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNA32937.1"
                     /translation="FTYNGKKYTPQSFAKEVVKVDPTDYAEFQSVTDIPYYSKGTLMV
                     PDNWALQQAWNIPVEDITAIIDNALRNGYSVSWGGDVSEPSFSWKNGVAFVTTTPAAD
                     LTADQKANLFKGPQPEPAITAEMRQKAFDNFTTTDDHGMQITGIATDQNGTEWYVVKN
                     SWGTKNQQEGYLYMSKAFAAYKMTAFMVNKNAVPAAIAKKLGW"
BASE COUNT          424 a          803 c          681 g          481 t
ORIGIN      
        1 aaggccgacc cgctgtacaa gcccactacg atgcccccga tgatcccggc gatgaggccg
       61 gcgatgagcg gggcgccact ggccagcgcg atgcccaggc aaagcggcat ggccacaagg
      121 aacacgacga ctgatgaggg cacatcgctg cgccacgaac tgaacaggtt cttcatgaaa
      181 attcggaata ggtggatgcc tgtgttgcat gccacatgga atgcggcatg ggcgaccagc
      241 catgggctga tcacacgcac gaaacgagca tctacctggg gggctgatcc ggcacttcca
      301 gcaccggatg gtccagcatt tcctcctcga actggaggaa acgcgccgtc atcaacggtt
      361 ctggatccgg aatgcgaacg tgctcctcga agggaacggc atgcttcagc acttcttcct
      421 cgatgatcgg tggcggctgg gaaccacagt cctcgaaaat cgctaccacg cgctgcgagg
      481 tgagcatctg aggcagaacg aacaggtacg ccgacatcac caggctcaac acgcgcatgg
      541 gcatggacca gacggtgcgt ccgggacctc gctgttgcac gtgggagaaa ttcatggggc
      601 gcaagttagt ccagccacgc acgacccgaa tatgttttgc aggcattaac ggaagagcac
      661 tgcccggcgg caactcagtt cccagcgatc gggtatatca ggtcgaacgc cgggatcgtg
      721 acatcgaacg tgctgtcgtc gtcggtatgc cgcatgcggt agctgccgtt catacgaccg
      781 aaaccgctgc gcaggtcgca gaagctgctg tacgtgaact gctcaccggg cggcaacacg
      841 ggtgtttcac ccaccacacc gggccccttg atctcccgtg gcccctgcaa gctgtccacg
      901 atgtgccagt gccttcgcaa caattgcacc gttcggccgc tgcggttctc gatgcggatc
      961 cggtaactga agaggaaccg gcccaccttc gggtcgctct gctcttcgga gtacgtcgcc
     1021 ttcacgctga cgcggatgtc gtgtgtgacc tgggtcgcca ttgcggtacg gttttggtcc
     1081 tcggccgatg caagtctaag tgacctcgcg atcctccgca tctttcgccc tccaagtttc
     1141 aacagcacga tgttccgttc gccctcctgc gtgctcctgc tcgcactcgc cacggccacc
     1201 ttcgcacagc ggccgaccgc caccttggta aaccccgggc tcatgcgcct ggacctcgaa
     1261 ggcaaccgcc ttttcgcggg tctgcccgac ccttccatcg gcgtggacga tatcgccctc
     1321 ttcccggtgg aacccgccag cccggttgct ggcgggcgca ccttcatgcc ctccatggaa
     1381 ggcgaactgc tcgaccgccg gaccgagcgc cttggcgaga agaccgcgat cttcgtacat
     1441 gccgtgccca cgccgtacgg cgataccgcc cactattcat acctggtttc ctggaacgac
     1501 cgccgcatct ggttcagtgg tctttccacc gaccccaccg ccctgctcgc cgccacggac
     1561 ctggacctgg ttttcctttc ccccgccatg gcccgtgcca tcgaggcgac cggcaaacgc
     1621 atgaacaccc gcatgatgat gctctatccg gtgcccgaag gatacgaccg catcaacgcc
     1681 accgggccct gcgactactg caaggtgctg gcccccgccg tgggcgagtc catccaattg
     1741 ttccgctgat cacacaccgc tacgcctcgc cccgccctac cagcccaact tcttggcgat
     1801 ggcggccggc acggcgttct tgttcaccat gaaggccgtc atcttgtacg ccgcgaaggc
     1861 cttgctcatg tacaggtagc cctcctgctg gttcttcgtc ccccagctgt tcttcaccac
     1921 gtaccactcg gtgccgttct ggtcggtggc gatcccggtg atctgcatgc cgtgatcgtc
     1981 ggtcgtggtg aagttgtcga acgccttctg tcgcatctcc gccgtgatgg ccggttcggg
     2041 ctgcggccct ttgaacaggt tcgccttctg gtcggcggtg agatccgcgg cgggcgtggt
     2101 ggtcacaaag gccaccccgt tcttccagct gaacgagggc tcgctcacat cgccgcccca
     2161 gctcacgctg tacccgttgc gcaacgcatt gtcgatgatg gccgtgatgt cctccaccgg
     2221 tatgttccac gcttgctgca acgcccagtt gtcggggacc atcagcgtac ccttgctgta
     2281 gtacgggatg tccgtcacgc tctggaactc ggcatagtcg gtcggatcca ccttcaccac
     2341 ttccttcgcg aagctctgcg gggtatattt cttcccgttg tacgtgaag
//
LOCUS       DAOQOC010000412         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26894_length_2382_cov_2.732703, whole genome shotgun sequence.
ACCESSION   DAOQOC010000412 DAOQOC010000000
VERSION     DAOQOC010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26894_length_2382_cov_2.732703"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PL106_07425"
     CDS             complement(<1..207)
                     /locus_tag="PL106_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32938.1"
                     /translation="MPGLINGSCGDRYGRGSGTPEVGDRARTHCGGITWPAARGHQPV
                     RYTMGDVLNYIGGTLRTAVGGTWLN"
     gene            268..1740
                     /locus_tag="PL106_07430"
     CDS             268..1740
                     /locus_tag="PL106_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA32939.1"
                     /translation="MGKRLIIGLTVAISIALAGLVIIQWRWISATLVLKDQQFNENVD
                     NALATVSDRLERIEAAERFRTHDKGREMLARMDSLARSEERDLMEHIIAMPPPIDEEA
                     GQWEPGYNERDSLITDLVRGLFSTEIYGSIIERVDPHLLDSLVSDELRRRGITERYDH
                     GIFAEDGTSARMDLVDPKDSLILRNSPHSAQLFRNDLVGEPYWLRVHLPDQHKHVLRS
                     MWPLLAASALFLLMIIAAFGWTLRTIWLQKKLGEIKNDLVNNLTHELKTPISTIALAC
                     EALNDASISKSPEQVKGFTTMIRDENKRLGLLVESVLQSAVSDSGSMRLRPVDVDLHA
                     VLAEVVRTSAISAANKGGRVELQADADLAHVRGDRIHLANVFFNLVDNAVKYCETAPV
                     VVLSTSSDVKGVQVAVKDNGIGIPKSEQKKIFDRLYRVPTGDRHNVKGFGLGLSYVRS
                     VVERHGGTIRVESGPGPGSTFFVTIPFEHGHPDKITGMRG"
     gene            1703..>2382
                     /locus_tag="PL106_07435"
     CDS             1703..>2382
                     /locus_tag="PL106_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA32940.1"
                     /translation="MDTPIKLLVCEDDPNLGSLLAQYLLAKGFVVEHRKDGEQGWAAY
                     AEGGFDLLVLDVMMPKLDGFSLARRIREKDARTPIIFLTAKSMKQDTITGFQAGADDY
                     LTKPFSMEELILRINAVLKRARGLAPKEEEPTRYALGSFVFDLRKQTLARDTSQSSLF
                     PGEEHDVTLTEVEANRKLTTKETELLRMLCMHRNALLPRETALKEVWGDDNYFNGRSM
                     DVYIAKLR"
BASE COUNT          501 a          727 c          747 g          407 t
ORIGIN      
        1 gttgagccag gtcccgccga cagcggtccg cagcgtacct ccgatgtagt tgagaacgtc
       61 gcccatggtg taccgcaccg gctgatggcc acgcgcggcg ggccaagtta ttccgccaca
      121 gtgcgtgcga gcgcgatccc ccacctccgg agttccggat ccccgaccgt accggtcgcc
      181 gcacgatcca ttgatcagcc ctggcatcag gttaattttg gttgatcacc cgttcagccc
      241 acggccggcc ggtagcttgg caccttcgtg ggcaagcggc tgatcatcgg gcttacggtg
      301 gccatctcca tcgcgttggc gggactggtg atcatccaat ggcgatggat cagcgccacc
      361 ctcgtgttga aagaccagca attcaacgag aacgtggaca atgcactggc cacggtgagc
      421 gaccgcttgg agcgcatcga agcggccgag cggttccgca cgcatgacaa aggccgtgag
      481 atgctcgcgc ggatggactc cctggcacga tcggaggaac gggacctcat ggagcacatc
      541 atcgccatgc ccccaccgat cgacgaggag gccggacaat gggagccagg atacaatgag
      601 cgcgattcac tgatcaccga tctggtgcgc ggcctcttca gtaccgagat ctatggcagc
      661 atcatcgagc gggtggaccc gcacctgctg gattccctgg tgagcgatga gttgcgccgc
      721 cggggcatca ccgagcggta tgaccacggc atcttcgcgg aagatggcac ctcggcacgg
      781 atggacctgg tggatcccaa ggactcgctc atcctgcgca acagcccgca cagcgcacag
      841 ctcttccgga acgacctggt gggcgagccc tactggttgc gcgtgcatct acctgatcag
      901 cacaagcacg ttctgcgcag catgtggccc ctgctggcgg ccagcgccct cttcctgttg
      961 atgatcatcg cggctttcgg ttggaccttg cgcaccatct ggctgcagaa gaaactgggc
     1021 gagatcaaga acgacctggt gaacaacctc acccatgagt tgaagacacc gataagcacc
     1081 atcgcgctgg cgtgcgaggc gctcaatgat gcgagtatca gcaagagccc cgagcaggtg
     1141 aagggtttca ccaccatgat ccgtgacgag aacaaacgtc tgggactgct ggtggaaagc
     1201 gtgttgcaga gcgcggtgag cgacagtggc agcatgcggt tgcgaccggt ggacgtggac
     1261 ctgcacgcgg tgctcgccga agtggtgcgt acgagtgcca tcagcgcggc gaacaagggg
     1321 gggcgggtcg agttgcaggc ggatgccgac ctggcacatg tacggggcga ccgcatccac
     1381 ctggccaacg tctttttcaa tcttgttgac aatgccgtga agtactgcga gaccgcaccg
     1441 gtggtcgtcc tcagcacatc cagtgatgta aagggcgttc aggtggcggt gaaggacaac
     1501 ggcataggca taccgaaaag cgaacagaaa aagatcttcg accgcctcta ccgtgtgccc
     1561 actggcgacc gccacaacgt gaagggcttc ggactgggcc tgagctatgt gcgcagcgtg
     1621 gtggagcgtc atggcggcac catccgggtg gagagcggtc ccggccccgg cagtacgttc
     1681 ttcgtaacga taccctttga acatggacac cccgataaaa ttactggtat gcgaggatga
     1741 cccgaacctc ggcagcctgc tggcgcagta cctcctggcc aagggcttcg tggtggagca
     1801 tcgcaaggac ggcgaacagg gttgggcggc gtacgcggag gggggcttcg acctgctcgt
     1861 actcgacgtg atgatgccca agctcgacgg cttcagcctg gcccgacgca tccgcgagaa
     1921 ggatgcgcgc acgccgatca tcttcctcac ggccaagagc atgaagcagg acacgatcac
     1981 gggcttccag gccggggccg atgattacct caccaagccc ttcagtatgg aagaactcat
     2041 cctgcgtatc aatgcggtgc tcaaacgggc gcgcggcctg gcccccaagg aggaggaacc
     2101 gacccggtac gccctcggga gcttcgtctt cgacctgcgg aaacagaccc tcgcacgcga
     2161 caccagccag agctcattgt tccccgggga ggagcatgat gtcaccctga cggaagtgga
     2221 ggccaaccgc aagctcacca ccaaggagac cgaactcctg cgcatgctct gcatgcaccg
     2281 caacgccttg ctaccacgtg aaacggccct gaaagaggtg tggggcgacg acaactactt
     2341 caacggtcgc agcatggacg tgtacatcgc caagctgcgc aa
//
LOCUS       DAOQOC010000413         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_26909_length_2381_cov_3.975064, whole genome shotgun sequence.
ACCESSION   DAOQOC010000413 DAOQOC010000000
VERSION     DAOQOC010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26909_length_2381_cov_3.975064"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="PL106_07440"
     CDS             complement(<1..1713)
                     /locus_tag="PL106_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNA32941.1"
                     /translation="MTRLTTLMLMLSAPVAATCQVSFGPAQVIHASQIDTKELACGDI
                     DGDDDNDVLISYNLAGEVYWHANLGQGIFGERIPVTTNLPNAQSPRLADLDGDGDQDA
                     VVVSPSTNVVHWFVNDGNGNYSAGSPLPFNGAANDAIACDLDGDGDQDMVVTGPDPGV
                     VLYSLNDGSGTFGAVQVVTDLLPGAWRAACADIDLDLDQDVVVISAVDGSIHWYPNLG
                     NASFAPGQEVPATMASPFIFDIFDLEGDGDPDILAPGEGLTWFENDGAGLFSPRISDG
                     LEATAGAVAAGDQDGDGLAEIYAEAEETDLGARHLVKAELDGNGNNNAWALIELGYVN
                     GTTDLEILDIDGIGEADVVVALENSGMVRWFQMVSGGPVSEQHIITANVLTQPMNLNV
                     IDMDADGDADIATAPPFQRTAWFENDGNASFLLRSTPILSEASVMPGVFFAADLDGNG
                     AGDLISQQYDSYSYPRFRAHLNDGSGLVFSPFGIVPASEPKPYFSTLADMDDDGDTDI
                     LYSPGWSVEDQVIRWRENGGMGQGWADHVVVSSSYDQVYDFRSMATGDLNGDGLLDII
                     GGNAN"
     gene            complement(1807..>2381)
                     /locus_tag="PL106_07445"
     CDS             complement(1807..>2381)
                     /locus_tag="PL106_07445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HNA32942.1"
                     /translation="QLESEGKAVHVLPLGKKALDTYRRTKYFNPDLPTDGASLYDGLS
                     FDKVAPKAELIMRLFADGSYDQVILVYNKFKNAAVQVLTEEQSLPVAPPAKGTADARS
                     AKADHIMEPSREEIVQNIIPKSLKIQLYKALLDSHAAEHGARMTAMHKATDNADALLK
                     DLKLSYNKARQAAITGEILEIVGGAEALKG"
BASE COUNT          458 a          740 c          693 g          490 t
ORIGIN      
        1 gttggcattc ccgccgatga tgtccagcag gccatcgccg ttcagatcac cggtagccat
       61 ggagcggaag tcatagacct ggtcatagga agagctcacg acgacatggt cggcccatcc
      121 ttggcccatg ccaccgttct ctcgccagcg gatcacttgg tcctctacgc tccatcccgg
      181 tgaatacaga atgtcggtgt cgccatcgtc atccatgtcg gcgagggtgc tgaagtaggg
      241 cttgggttca ctggccggta cgataccgaa cggactgaac acaagtccgg aaccatcgtt
      301 cagatgtgcg cggaaccgtg gataggaata gctgtcgtac tgttggctga tcaagtctcc
      361 cgcaccgttc ccatcgagat ccgcagcgaa gaacacgcct ggcatcaccg aggcttcact
      421 caggatcggt gttgaccgca ggagaaatga agcgttgcca tcgttctcga accaggcggt
      481 tcgttggaaa ggaggagcgg tagcgatgtc cgcatcgcca tcagcatcca tgtcgatcac
      541 gttcaggttc atgggttggg tgaggacatt ggccgtgatg atgtgttgct cggataccgg
      601 acctccactc accatttgga accatcggac catacccgaa ttttcgagcg cgaccacaac
      661 atccgcttca ccgatgccat cgatatccaa gatctccagg tccgtggttc cgttcacata
      721 tccgagctcg atgagagccc atgcgttgtt gttcccgtta ccgtcgagtt cggctttcac
      781 cagatggcgc gcaccgaggt cggtttcctc ggcctccgca tagatctccg ccaggccatc
      841 cccgtcctgg tcccctgccg ctaccgcgcc tgcggtagcc tccaatccat cgctgatacg
      901 gggggagaac agcccggccc catcgttctc gaaccaggtc agtccttccc ccggggccag
      961 gatatcggga tcaccatcac cctccaggtc gaagatgtca aagatgaaag gggaagccat
     1021 ggtcgcgggc acttcctggc ccggggcgaa ggatgcgttc cccagattcg ggtaccagtg
     1081 gatcgacccg tccacggcgg agatgaccac gacgtcctgg tcaaggtcca gatcgatatc
     1141 ggcacaggct gcacgccacg ctccgggcag gaggtccgtc acgacctgaa cggccccaaa
     1201 ggttccggag ccatcattga gtgaatagag aaccactccc ggatccgggc cggtcacgac
     1261 catgtcctga tccccgtcgc cgtccagatc acatgcgatc gcatcattgg cggcaccgtt
     1321 gaacgggagc ggggaacctg cgctatagtt accattccca tcgttcacga accagtggac
     1381 cacattggtg gatggagata cgaccaccgc atcctgatcc ccgtcgccgt ccagatcggc
     1441 gagccgcgga ctctgcgcat tgggcaggtt ggtcgttacc gggatccgct cgccgaagat
     1501 cccctggccg aggtttgcat gccagtagac ttctccggcg aggttgtaac tgatcaacac
     1561 gtcattgtcg tcatccccat cgatgtcccc gcaagccagt tccttcgtat cgatctgcga
     1621 cgcatggatc acctgggccg gcccgaagga cacctggcat gtggcagcta ccggggcaga
     1681 gagcatcagc atcagagtgg tcagacgggt catgggtcca gcctttggaa tagccaaagt
     1741 agctcgtcca tggcatagga ccgatatgac tgcgacggcc cgcgaacgaa agctctcgcg
     1801 aagggatcag cccttcagcg cctccgcacc acccacgatc tccaggatct cgccggtgat
     1861 cgctgcctga cgtgctttgt tgtaggacag cttcaggtcc ttcaacagcg catcggcgtt
     1921 gtccgtggct ttgtgcatag ccgtcatccg ggccccatgc tcggccgcat ggctgtcgag
     1981 cagagccttg tacagctgga tcttcaagct cttcggaatg atgttctgca cgatctcctc
     2041 gcggctgggc tccatgatgt ggtccgcctt cgcgcttcgc gcatcggcgg tccccttcgc
     2101 gggcggggcc acgggcaggg attgctcctc ggtgagcacc tggacggccg cgttcttgaa
     2161 cttgttgtac accaggatga cctggtcgta cgagccgtcg gcaaaaagcc tcatgatcag
     2221 ctccgccttg ggggccacct tgtcgaagct caacccgtcg tagaggctcg caccgtccgt
     2281 gggcaggtcg gggttgaaat acttcgtgcg gcggtaggta tcaagcgcct tcttgccgag
     2341 gggcagtacg tgaacagcct tgccttcgct ctcgagttgc g
//
LOCUS       DAOQOC010000414         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27051_length_2375_cov_3.261207, whole genome shotgun sequence.
ACCESSION   DAOQOC010000414 DAOQOC010000000
VERSION     DAOQOC010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27051_length_2375_cov_3.261207"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1951)
                     /locus_tag="PL106_07450"
     CDS             complement(56..1951)
                     /locus_tag="PL106_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32943.1"
                     /translation="MYRPTLPRRRTGFLGARSVLLLSIGWYLLFAAPALAQTGANDPG
                     VLTTRDKALINTAAERLIGSYTDILNMFSRYDASSSSDVEDVKLLVAAITQGEDRIFT
                     DTTAIIESDLDPSIQPGKAVPNLTAPVYLLDFFTVFHGDVQNPITMRLADYGEPHTTP
                     DGLVVTSLLYNVSWQGSNKNTGTSYTPHQRVILFTAARQGPKDWVVHIATDSWYDPAN
                     PFKSYRKELEAERARSKDRIEAVLKEYEDEARQAASEAARSLEERTASYDAAIAKGDA
                     LLNDDPEGAMILYDKARDLAPVGRLDHLLQSRKAQRALDAKNTSERERALDTLSARLV
                     RLVVHGGFRSVTILDMTETNGVKSALGSHLSNELAGRMARMASHFTVSRGLAAGRGGA
                     AIGGNLMRVGGNRIGLTATLQDKRGNVRGQEKEDLPMPEGFDQGGAEPNNDDLTSRNK
                     APGLWSVGVSVGLNLTRISGRNVLSDRFTENGIGPRAGIDVERMGATGIHRFATGVRW
                     ASFNVSTTGDDVSKQLKVDYVQVPLLYRIFTGRTAIGRFGLQAGVLADFTVGTLSESY
                     TSKEYIDVSFLNAHASGGLCYETNGPRRTRITANVSYDPMILAGIETGHRTAAGDLKT
                     DQDGPGC"
     gene            2050..>2375
                     /locus_tag="PL106_07455"
     CDS             2050..>2375
                     /locus_tag="PL106_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32944.1"
                     /translation="MNRYFPFFVLDVMTHGIMIRPLHGSGALCAVVAVLLAGACKKEF
                     LTVVEAAPAGATPSAIISDSSNVPILLDNCAGENVLELELDATQLHAGDRIQAVFSVG
                     LSQANGS"
BASE COUNT          414 a          716 c          741 g          504 t
ORIGIN      
        1 attgctcgca gacacgggcc gaatctgacc tgctgcgcgg ctgccaaccc gattttcaac
       61 agccaggacc atcctggtct gttttcaaat cgcctgccgc tgtcctgtgc cccgtctcga
      121 tcccggcgag gatcatcggg tcatacgata cgttggcggt gatccgcgta cgccttgggc
      181 cattggtctc gtaacacagg ccgcctgaag cgtgcgcgtt caggaagctc acgtcgatat
      241 actctttcga ggtatagctt tccgagaggg taccgaccgt gaagtctgcc aatacgccag
      301 cttgcagacc gaagcgaccg atcgcggtcc gaccggtgaa gatcctgtag agcaagggga
      361 cctgcacgta atccaccttg agttgcttgc tcacgtcatc gccggtggtg gagacattga
      421 aagaggccca acgcaccccg gtggcgaagc gatgaatgcc cgttgctccc atccgctcca
      481 catcgatccc tgcccgcggg ccgatcccat tctcagtgaa gcggtccgac aggacgttcc
      541 gaccgctgat gcgggtcagg ttcaggccga ctgaaacccc cacggaccac agtcccggtg
      601 ccttgttccg actcgtcagg tcatcattgt tcggttccgc accgccctga tcgaatcctt
      661 caggcatggg gaggtcctcc ttttcctggc cgcgcacgtt accacgtttg tcctgcaggg
      721 tggccgtgag gccgatcctg ttgccaccta cccgcatcag gttaccgccg atcgcggctc
      781 cgccacggcc agctgccaag ccgcgtgata ccgtgaaatg cgaagccatg cgcgccatgc
      841 gtccggccag ttcgttggac aggtgcgagc ccagggcact cttcacaccg ttcgtctcgg
      901 tcatatccag gatcgtcacc gaccggaagc ctccatgcac caccaggcgg acgagtcgag
      961 cgctcaaggt gtccaaggcc cgctcacgtt cgctggtgtt cttcgcgtcc agggcgcgtt
     1021 gtgctttccg gctttggagg aggtggtcca agcgacccac cggagcgaga tccctggcct
     1081 tgtcgtacag gatcatggct ccttcagggt catcgttcag cagggcgtcc cctttggcga
     1141 tcgcagcatc gtagctcgcg gtgcgttcct ccagtgatcg tgcggcctcg ctggcggctt
     1201 gcctggcctc gtcctcgtat tccttcagca cggcctctat gcggtccttg ctgcgcgcac
     1261 gttcggcctc caactccttt cggtagcttt tgaacgggtt ggccgggtcg taccagctgt
     1321 ccgtggcgat atgcaccacc cagtccttgg ggccttggcg tgcggccgtg aacaggatca
     1381 cacgctgatg aggggtgtag gacgtgcctg tgttcttgtt cgagccttgc caggacacgt
     1441 tgtagagcaa actggtcacc accagtccgt ccggcgtggt gtgcggctcg ccgtaatccg
     1501 ccaaccgcat ggtgatcggg ttctggacat cgccatggaa cacggtgaag aaatcgagca
     1561 gatacaccgg ggcggtcagg ttcggaaccg ccttgccagg ctggatggag gggtcgagat
     1621 cgctttcgat gatcgcggtg gtatcggtga agatccgatc ctcgccttgg gtgatcgcgg
     1681 ccaccaggag tttcacgtcc tccacatcgc tgctactgct cgcatcgtaa cggctgaaca
     1741 tgttgaggat atcggtatag ctcccgatca accgctccgc agcggtgttg atcagggcct
     1801 tgtcacgggt ggtgagtacc cccgggtcgt tcgcgccggt ttgtgcgagg gcaggggccg
     1861 cgaacaacag gtaccagccg atggagagga gcaggaccga acgggcccca aggaagccgg
     1921 tgcgcctcct gggaagtgtg ggccggtaca tggtacaggg atcgcgccga aggtagcgcg
     1981 cgaaccgatc caactgttgg atacccgggt ccggatggtc cggaaaggag tgctcaggaa
     2041 ataccgggaa tgaaccggta ttttcctttc tttgtactgg acgtaatgac gcatgggatc
     2101 atgattcggc cgttgcatgg atcgggcgcg ctctgcgcgg ttgtggcggt acttctggcg
     2161 ggtgcgtgca agaaggagtt ccttactgtg gtcgaagcgg cacctgcggg tgcaactccc
     2221 tccgcgatca tctcagatag ttcgaatgtg ccgatcctcc tggacaactg cgcgggcgag
     2281 aatgttctgg aactggagct ggacgcgacc cagttgcatg cgggtgatcg gatccaggcg
     2341 gtgttctcgg tcggtctttc gcaagcgaac ggatc
//
LOCUS       DAOQOC010000415         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27116_length_2371_cov_3.847150, whole genome shotgun sequence.
ACCESSION   DAOQOC010000415 DAOQOC010000000
VERSION     DAOQOC010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27116_length_2371_cov_3.847150"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="ribB"
                     /locus_tag="PL106_07460"
     CDS             complement(<1..893)
                     /gene="ribB"
                     /locus_tag="PL106_07460"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNA32945.1"
                     /translation="MAHEGEFDDIESAIEDIRQGKVVIVVDDEDRENEGDFLTAARNA
                     TPEVVNFMATHGRGLICAPLTEQRCAELGLDLMVRDNTALHETPFTVSVDVRGHGTTT
                     GISAYDRAQTMRALIDPATVPADLARPGHIFPLKARDGGVLRRTGHTEAAVDLARLAG
                     LEPAGMIVEILNEDGSMARLPELRAIARRFNLKLITIEALVAYRMRNERLVERVVDVD
                     LPTDHGDFKLIAYKQRDNGEEHLALVKGEWSPGEPVLVRVHSSCVTGDIFGSQRCDCG
                     PQLHAAMELVERAGKGVVLYM"
     gene            complement(903..2093)
                     /locus_tag="PL106_07465"
     CDS             complement(903..2093)
                     /locus_tag="PL106_07465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA32946.1"
                     /translation="MKLLVVLSRVPYPLEKGDKLRAYHLVTRLARHHRICLFCLTDQR
                     IDPAHIAHLREHCERIEVVYLPRWRILASLATTVFTRLPFQVAYFHHSTAQRAIDRVI
                     ASFAPDHVLCQLVRTTEYVRHRYDLPKTLDYMDTLSKGLERRIENAGFWLRPLMRIET
                     RRLITYENLMLDLFDGHAIISAQDRELLYHPMRDQVKVIPNGVDTGFFSPRAATRTHD
                     LLFTGNMNYPPNIDSVLYLVHRVLPLVRRERPDTSLLISGVDPTPRVRELAKDDPLIT
                     VSGRVPDIRDAYAAATVFAAPMQIGTGLQNKLLEAMAMRMPCVTSALANNAVGAVPGE
                     SILIGGTPEEYAAHIVRLLGDEPERARIAENGFRFVRENFDWERNAEALDRLIGAAHP
                     PAAP"
     gene            complement(2090..>2371)
                     /locus_tag="PL106_07470"
     CDS             complement(2090..>2371)
                     /locus_tag="PL106_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA32947.1"
                     /translation="DVVSPLKPLEPMAMGKCVISTAIGAEGIGYTDGHDLLLANSATA
                     MAGIIGSLIEDPARARTIGLNARQLVLKQYTNDGIVKDLVAFFGSLPEA"
BASE COUNT          398 a          758 c          761 g          454 t
ORIGIN      
        1 tgcatgtaca gcaccacgcc cttgcccgca cgctccacca gttccatggc ggcatgcaac
       61 tgtggaccgc agtcgcatcg ctggctgccg aaaatgtcgc cggtgacgca actgctgtgt
      121 acgcggacga gcacgggctc gccgggcgac cattcgcctt tcaccaaggc caagtgctcc
      181 tcaccgttgt cgcgctgctt gtacgcgatg agtttgaagt cgccatgatc agtgggcagg
      241 tccacatcaa ccacccgctc aaccaggcgc tcgttgcgca tccggtacgc cacaagtgct
      301 tcaatggtga tcagtttcag gttgaagcgc cgggcgatgg cgcgcagttc gggcaggcgg
      361 gccatgctgc cgtcctcgtt caggatctcc acgatcatgc cggcgggttc aaggccggcc
      421 agccgggcca gatccacggc ggcttcggta tggcccgtgc gtcgcagcac gccgccatcc
      481 cgtgccttca acgggaagat gtggcccgga cgcgccagat cagcgggcac cgtggccggg
      541 tcgatcaagg cccgcatggt ctgcgcccgg tcgtacgcac tgatgcccgt ggtggtaccg
      601 tgtcctctga catccacgct caccgtgaac ggtgtttcgt gcaacgcggt gttgtcgcgc
      661 accatgaggt ccagtccaag ttcggcacac cgctgttcgg ttaggggcgc gcagatgagc
      721 ccgcggccat gggtggccat gaagttgacc acttcgggcg tggcgttgcg cgcggccgta
      781 aggaagtcac cctcgttctc ccggtcctca tcgtccacca cgatcaccac tttgccctgc
      841 cggatgtcct cgatcgcgct ctcgatatca tcgaactccc cttcgtgtgc catgccgcga
      901 agctacggcg ccgcaggtgg gtgtgccgcg ccgatcagcc tgtccagcgc ttcggcgttc
      961 cgctcccagt caaagttctc ccgaacgaac ctgaacccgt tctcagcgat ccgcgcgcgc
     1021 tccggttcgt cgcccaacag gcgcacgata tgagcggcgt attcttccgg cgttcccccg
     1081 atcaggatgc tctctccggg tacggcgccc acggcgttgt tggcaagggc actggtgacg
     1141 cagggcatgc gcatggccat ggcctccagc agtttgttct gcagcccggt accgatctgc
     1201 atgggcgcgg cgaaaaccgt ggcggccgcg taggcgtccc ggatgtcggg cacccgtccg
     1261 ctcacggtga tcagcggatc gtccttggcc agctcgcgta cccggggcgt ggggtccaca
     1321 ccgctgatga gcaggctggt gtcgggccgc tcgcgacgca ccagcggcag cacgcgatgc
     1381 accaggtaaa gcacgctgtc gatattgggc ggatagttca tgttgcccgt gaagagcagg
     1441 tcgtgcgtcc tcgttgccgc ccgcgggctg aagaaccccg tatccacccc gttcgggatc
     1501 actttgacct ggtcgcgcat cggatggtac aacaactcgc ggtcctgcgc gctgatgatc
     1561 gcatgaccgt cgaacaggtc gagcatgagg ttctcgtacg tgatcagcct gcgggtctcg
     1621 atcctcatca acggccgaag ccagaagccg gcattctcga tgcgacgctc caagcccttg
     1681 ctcagcgtgt ccatgtaatc gagtgttttc ggcaggtcgt acctgtggcg gacatactcg
     1741 gtggtgcgta ccaattgaca cagtacatgg tcgggggcga acgatgcgat cacccggtcg
     1801 atcgcacgct gcgctgtgct gtgatggaag taggccacct ggaacggcag gcgggtgaat
     1861 acggtggtgg ccaggctggc caggatgcgc cagcgtggca ggtacacgac ctctatccgt
     1921 tcgcagtgtt cccgcaggtg cgcgatgtgg gccggatcga tccgctggtc ggttaggcag
     1981 aacaggcaga tccgatggtg gcgtgccagg cgggtgacca ggtggtaggc gcgcagcttg
     2041 tcgcccttct ccagcggata gggcacacgg gagaggacca ccagcagctt catgcctcgg
     2101 gcagggagcc gaagaaggcg acaagatcct tcacgatccc gtcgttcgtg tattgtttca
     2161 aaacgagttg ccgggcattc aggccgatcg tgcgcgcacg tgccgggtcc tcgatcaacg
     2221 aaccgatgat cccggccatg gccgttgcgc tgttggccag aaggaggtcg tgcccatcgg
     2281 tgtatccgat cccctcggcg ccgatcgcgg tgctgatcac gcacttgccc atggccatag
     2341 gctccagagg tttgagcggc gacacgacgt c
//
LOCUS       DAOQOC010000416         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27168_length_2369_cov_3.015557, whole genome shotgun sequence.
ACCESSION   DAOQOC010000416 DAOQOC010000000
VERSION     DAOQOC010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27168_length_2369_cov_3.015557"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..650
                     /locus_tag="PL106_07475"
     CDS             129..650
                     /locus_tag="PL106_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNA32948.1"
                     /translation="MEKIARPVPGDYPAYHGAYLLAAKGNDLAEALRHASEEEARTCQ
                     LIPDDRWEHRYAPGKWTIKEVFQHIIDTERVFSYRALCIARGEQANLPAMDEDAYQAM
                     ARTSTRRVEDLVRELAAVRLSTMELFDSFDAQALACSGTANGKHVTVPALGFIIAGHS
                     EHHLRIIRERYLA"
     gene            662..1132
                     /locus_tag="PL106_07480"
     CDS             662..1132
                     /locus_tag="PL106_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNA32949.1"
                     /translation="MPARSIQQWLDEYGESHQNGTNELIHWICVPVIFFTVIGFFASI
                     PSPVLPVAGGDLWAKLAMVGCLAFYWMRSIPLFVGMALWCLFCLGLAMRLDTHAGRPL
                     WAICLVLFTVAWIGQFIGHHIEGKKPSFLKDLQFLLIGPAWLMSKLYAKLGIGY"
     gene            complement(1126..2286)
                     /locus_tag="PL106_07485"
     CDS             complement(1126..2286)
                     /locus_tag="PL106_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA32950.1"
                     /translation="MLLSNLLLGCGGPPPTADTSGWATWRRVDNAYAERFQIWERGAD
                     RLLLVFGHGGIQDTVGRYVVTTDDPSPLPVSALQLTPGLKEVATGSTTHVPYIAALGA
                     LEAIAACAHTDQVRDTVFASRVRHGLVREIALGDGLDREVLIAMRPRIFFGYPFGRGD
                     AGALEQAGIPVVEVSEYLEEHPLGRAEWLRFFGVLLGRERLADSLFSGIRQRYEAIRA
                     DSTTRDRPSVLFGSVWSGQWWVPPGNSYMARFIEDAGGRYVFADRTGHGNIAVDMETM
                     ITVGAQAERWGMIAQLRYPVVLSDFTGGDPRLEAFRSVEQNGLFIGNSSQADLFGQAL
                     VEPDRELLDLIIALHPDGDYFQRDLPVVPAGYFRHVEWPVIPTVSFGNAEAQ"
BASE COUNT          439 a          798 c          721 g          411 t
ORIGIN      
        1 gcagaaccga cagccgtcga gttggtccaa tgggagaagg gatcgtgaac ctcctcctgc
       61 ccgccgcaca ggccaaccgg atgcgccatc cgtgttgacc tgatcgttcc gcacctacct
      121 tcgggcgcat ggaaaagatc gcacgacccg taccgggcga ctatccggcg taccatggag
      181 catacctgct ggcggccaag gggaacgacc tggccgaggc gctccgccac gccagtgagg
      241 aggaagcacg cacctgccag ctgatcccgg acgaccgttg ggagcaccgg tatgctccgg
      301 gcaaatggac catcaaggaa gtcttccaac acatcatcga tacggagcgc gtgttcagct
      361 atcgtgcgct ctgcatcgcc cggggagagc aggccaacct gcccgcgatg gatgaggacg
      421 cgtatcaggc catggcgcgc acctccaccc gccgcgtgga ggatctcgtg cgcgaactgg
      481 cagccgtgcg tctgtccacg atggaactgt tcgacagctt cgacgcacaa gccctggcat
      541 gcagcggcac cgccaacggc aagcacgtga cggtgccggc gctcggcttc atcatcgccg
      601 ggcacagcga acaccacctg cggatcatcc gtgagcgcta tcttgcctga acgtgtccga
      661 gatgcccgcc cgcagcatac agcagtggct cgatgagtat ggtgagagcc accagaacgg
      721 gacgaacgaa ctgatccatt ggatctgcgt tcccgtgatc tttttcacgg tgatcggctt
      781 cttcgccagc atcccttcac ccgtgctccc tgtggcgggc ggggacctgt gggccaaact
      841 cgccatggtc ggctgcttgg ccttctactg gatgcgctcc atcccgttgt tcgtcggcat
      901 ggcgctctgg tgcctcttct gcctgggact ggccatgcgc ttggataccc atgcgggccg
      961 gcccctgtgg gccatttgcc tggtcctctt cacggtggcg tggatcggcc agttcatcgg
     1021 ccaccacatc gagggcaaaa agccgagctt cctcaaggac ctccagttcc tgctgatcgg
     1081 accggcctgg ttgatgtcga agctctacgc gaaactgggg atcggctact gagcctccgc
     1141 gttgccgaag gataccgtgg gtatcaccgg ccattcgacg tgcctgaagt acccggcggg
     1201 caccacgggc aggtcgcgct ggaaatagtc cccatccggg tgcagtgcga tgatgaggtc
     1261 cagcagttcg cgatcgggtt ccaccagggc ctggccgaag agatccgcct gcgagctgtt
     1321 cccgatgaac aggccgttct gttctacgga acggaaggcc tccagtcgcg gatcgccacc
     1381 ggtgaaatcg gacaggacca ccggataccg caattgtgcg atcatgcccc agcgttcggc
     1441 ttgtgcgccc acggtgatca tggtctccat gtccaccgcg atgttcccat ggcccgtacg
     1501 gtccgcaaaa acataccggc cgccagcgtc ctcgatgaac cgggccatgt aactgttgcc
     1561 gggcggcacc caccactggc cgctccacac gctgccgaag aggacggaag ggcgatcccg
     1621 cgttgtgctg tccgcacgaa tggcctcgta ccgttgacgg atgccgctga acaggctgtc
     1681 cgccaaccgc tccctgccga ggagcacacc gaagaagcgc agccactccg cacgacccag
     1741 cgggtgctcc tccaggtact cgctcacttc aacgacaggt atgcccgctt gctccaaggc
     1801 gcccgcgtcc ccgcgtccga acggatagcc aaaaaatatc cgtggccgca tggcgatgag
     1861 cacctcgcga tcgagtccgt cgcccagtgc gatctcgcgt accaagccat gtcggacccg
     1921 ggaagcgaac acggtgtcgc ggacctgatc ggtatgggcg catgcggcaa tggcctccaa
     1981 cgctcccaag gccgcgatgt agggcacatg cgtagtgctg ccggtggcca cctccttcag
     2041 gccgggtgtc aattgcaagg cgctcaccgg gagcggtgat ggatcatctg ttgttaccac
     2101 ataccgaccg acggtatcct gtatcccgcc atgaccgaag acgagcagca gccggtccgc
     2161 gccccgctcc catatctgga accgctccgc gtacgcattg tccacgcggc gccacgttgc
     2221 ccagccgctc gtgtccgccg tgggcggcgg cccgccgcag cccaacaaca ggttgctcag
     2281 gagcatgatc gcaaggatcg accgggcgga acgcacggcg cgaatctatc ggcgggcccg
     2341 ggccgaccat ccgctcactg atcggctcc
//
LOCUS       DAOQOC010000417         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27335_length_2362_cov_3.098396, whole genome shotgun sequence.
ACCESSION   DAOQOC010000417 DAOQOC010000000
VERSION     DAOQOC010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27335_length_2362_cov_3.098396"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PL106_07490"
     CDS             <1..923
                     /locus_tag="PL106_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA32951.1"
                     /translation="SCAGDTTFQFDCLSVPGGPNMPGTSCDDLDSLNTNDTWSTACTC
                     EGEPFTIDCNGVVYGPDMPGTLCDDGDPFTTGDMWTASCLCIGYTPAPCAINFQVTQA
                     LAFDSATWTSMPIPGTLWVTFQSNGAWPYTYLWDFGDGTTSTEPYPTHTYPGFGPYNL
                     CVTITDGTGCTSTTCDSVAVDPDGFIILMGLGGGHVTAAHPRSGFTIIVLGDVPTTVA
                     GHQADEELTVWPVPAAEEIYMGIEQPSGERVQVRVLDINGRAVRPWGSPEVTGSDIVV
                     LRIDAVLEGMYTIQVKEGGRIRSACFVKVE"
     gene            complement(955..1218)
                     /locus_tag="PL106_07495"
     CDS             complement(955..1218)
                     /locus_tag="PL106_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32952.1"
                     /translation="MQSRIHAVKPLFDMPPEDVLVFRTSVENNDQVHALRPMLDELIA
                     RGGRWSFDLEDRDRILRVVTDAQVKEPLMALLRRHGHTCVELL"
     gene            complement(1274..1687)
                     /locus_tag="PL106_07500"
     CDS             complement(1274..1687)
                     /locus_tag="PL106_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32953.1"
                     /translation="MYHFRPRHLLFPLIPIAAVAVFSLVVMLLWNAIIPGITGWAVLS
                     YWKAMGLLVLCKILFSGPPGRRFGPGGGHWAGRRAGMRMAWWRNLSPEEREQWQERRK
                     EWRATWQGMDPEQRAKFKASWKARCGGWQEKNDPA"
     gene            complement(1706..>2362)
                     /locus_tag="PL106_07505"
     CDS             complement(1706..>2362)
                     /locus_tag="PL106_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA32954.1"
                     /translation="QCRWTSPFFSRPDGSVAYFAPPSVITSVGTDAMELTMARKQGGI
                     TRAVARYGRALFGFIRARVPTDADAEDLLQDVWAQLSLQPEVEAIDQMSGWLYRVARN
                     KIIDRYRKGSTEALDDLVTEDEEGTFSFGELLLAVEDDPDLRLLRDLFWQELEAALAE
                     LPENQRLVFVQNELEDKALRVIAEEQGENIKTIISRKRYAVQHLRERLETIYHELTRP
                     "
BASE COUNT          451 a          719 c          728 g          464 t
ORIGIN      
        1 gctcatgcgc aggggatacc accttccagt tcgattgcct gagtgttcct ggtgggccga
       61 acatgccggg cacgtcctgc gatgacctcg attcgctcaa cacgaacgat acctggtcca
      121 cggcatgtac ctgtgagggt gagccgttca cgatcgattg caatggggtt gtgtacgggc
      181 cggatatgcc gggcacattg tgcgatgacg gtgacccgtt cactaccggc gatatgtgga
      241 ccgcctcgtg cctgtgcatc ggctacacac ccgcgccttg tgccatcaac ttccaagtga
      301 cccaggcctt ggcgttcgac agtgcgacct ggacctccat gccgattccc ggaacacttt
      361 gggtaacatt ccaaagcaac ggtgcatggc cgtacacgta cctctgggat ttcggtgatg
      421 gcaccacctc cacggaaccc tacccgacgc atacgtatcc gggcttcggt ccgtacaacc
      481 tctgcgtcac catcaccgat ggtaccggtt gcacgagcac tacctgcgat agcgtagcgg
      541 tggatcccga tggcttcatc atcctcatgg ggctcggcgg tggccatgtc acagcggcgc
      601 acccgcgttc cgggttcacc atcatagtcc tgggtgacgt gccgaccacc gttgcgggac
      661 atcaggcgga tgaggagctg accgtatggc ccgtaccggc cgccgaggaa atatacatgg
      721 gtatcgaaca accgtctggt gaacgggtgc aggtgcgcgt actggacatt aacgggcgag
      781 cagtacgacc ttggggttca cccgaggtga ctggatcgga cattgtcgtc ctgcggatcg
      841 atgcggtcct cgaagggatg tacacgatcc aggtcaagga gggaggtcgt atccgctccg
      901 catgtttcgt caaggtggag tagcccttcg cgggaagaac gaagccgcaa cggatcagag
      961 cagttccacg caggtgtgac cgtgccgacg gagcagggcc atcaggggtt ctttgacctg
     1021 cgcatcggtc accacacgca ggatgcgatc gcggtcttcc aggtcgaagc tccagcgacc
     1081 acctctcgcg atcaactcgt ccagcatggg acggagtgcg tggacctggt cgttgttctc
     1141 cacggaagtc cggaacacaa ggacatcttc aggaggcata tcgaacagcg gcttgacagc
     1201 gtggatgcgg ctttgcatgg cagaatgaag gagcttcaac gtggcagacg gaccaatgcc
     1261 ccgaaaacct tgatcaagcg ggatcatttt tttcctgcca gcccccgcag cgggctttcc
     1321 aggacgcctt gaacttggcg cgctgctccg gatccatgcc ctgccatgtg gcccgccatt
     1381 ccttgcggcg ctcttgccat tgttcgcgtt cctccgggga caggttgcgc caccaggcca
     1441 tgcgcatgcc cgcacgccgg ccggcccagt gcccaccacc aggtccgaag cgccgccccg
     1501 gagggccgct gaagaggatc ttgcacagga cgagcaggcc catggctttc cagtaagaga
     1561 gcacggccca gccggtgatg ccgggaatga tcgcgttcca cagcagcatc accaccaggc
     1621 tgaagacggc aacagcagcg atggggatca gcgggaacag gaggtgacgg ggacggaagt
     1681 ggtacatggt attcgggttt tccggtcagg gtcgggtcag ttcgtggtag atggtttcca
     1741 aacgctcgcg caggtgctgc acggcgtaac gttttcggga gatgatggtc ttgatgttct
     1801 cgccctgttc ctcggcgatg acgcgcaggg ccttgtcctc caactcattt tggacgaaga
     1861 cgaggcgttg gttctcgggc aactcggcca atgcggcttc cagttcctgc cagaacaggt
     1921 cgcgcaacag gcgcaggtcg ggatcatcct ccacggccaa cagcagttcg ccgaaactga
     1981 aggtaccctc ttcatcttcg gtcaccaggt catccagcgc ctcggtactt cccttgcggt
     2041 accggtcgat gatcttgttg cgggccacgc ggtacagcca gccgctcatc tgatcgatcg
     2101 cctccacctc gggttgcagg ctcagttgtg cccacacgtc ctgcagcagg tcctccgcat
     2161 cggcatcggt gggaacccgg gcacggatga agccgaagag cgcccggccg tatctggcca
     2221 ccgcccgggt gatcccgccc tgcttgcgcg ccatggtcag ttccattgca tcggtcccca
     2281 cggatgtgat gacggatggc ggggcgaaat acgctaccga accgtccggc cgggagaaaa
     2341 atggtgatgt ccatcggcat tg
//
LOCUS       DAOQOC010000418         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27606_length_2350_cov_4.530719, whole genome shotgun sequence.
ACCESSION   DAOQOC010000418 DAOQOC010000000
VERSION     DAOQOC010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27606_length_2350_cov_4.530719"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..893)
                     /locus_tag="PL106_07510"
     CDS             complement(201..893)
                     /locus_tag="PL106_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA32955.1"
                     /translation="MATNAPMNLEMLQRVAEALGGDLERAVFVGGAVVSVYADDPAAS
                     EARPTYDIDLAVSVATAGELETLRGRLIAKGFTQSAADPVMCRFRIDGIMVDVMALEP
                     VGWAPGNRWYASGFPKRESHVLAGGPHIPVLPLAYFLASKFDAHADRGGHSLANRDLE
                     DIVHVLDNRLDIPSVWRNADPAVSVYLRDRLSWLVHPDQKEAVLGHMGFSASEARYQR
                     MRREVRMAIGGL"
     gene            complement(895..1386)
                     /locus_tag="PL106_07515"
     CDS             complement(895..1386)
                     /locus_tag="PL106_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32956.1"
                     /translation="MKPQDIVVLLSIALRERGTWKAKDLAADLVISPAEIGYSLKRSI
                     QSGLLSEDRELMSKGFLDMLYHAVRYVFPARPGAEVRGVPTAHSAPPLKQTIVSGPLF
                     VWPDPMGKARGAAIQPLYPTVPQAAAKNKDLYALLALTDALRTGRVRERELAMKELRE
                     RIR"
     gene            complement(1497..2225)
                     /gene="pyrH"
                     /locus_tag="PL106_07520"
     CDS             complement(1497..2225)
                     /gene="pyrH"
                     /locus_tag="PL106_07520"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNA32957.1"
                     /translation="MAKAKTERTYKRILLKLSGESLMGGKSYGIDDAMLKAYADEIIA
                     VAKGGTQVAVVIGGGNIYRGMNASGSGIDRVQGDHMGMLATMINSLALQSALEAKGQS
                     TRLQSAIKMEQIAEPFIRRRAVRHLEKGRIVIFGAGTGNPYFTTDTAASLRAIEIEAD
                     VILKGTRVDGIYTADPEKDKKAKKYDRITFDEAYDKGLSVMDLTAFTLCRENNLPIIV
                     FDMNKRGNLRKVVAGEKVGTLVEV"
BASE COUNT          394 a          789 c          710 g          457 t
ORIGIN      
        1 gcccgctcgg atcttcgtga gttcgtgatc caggtggtcc acggccttct tcatcaggtc
       61 ctcgttcggg gcgatcaggg tcttggcgtc catcgcattc gtgttggggt cgcgaaaata
      121 actggacgcg ggggttcacg ttcccatgac cgggcgagga cgcgcccgtt gacggacctc
      181 cgttcctatc cggatacggc tcacagacct cctatcgcca tcctgacctc ccgccgcata
      241 cgctggtagc gggcctcgct ggcggaaaac cccatgtggc ccaacacggc ttccttctgg
      301 tccggatgga ccaaccagga caatcggtcc cgcagataca cgctcacggc cggatcggcg
      361 ttccgccata ccgaagggat gtccaagcgg ttgtcgagca catggacgat gtcctccagg
      421 tcccggttcg cgagcgaatg cccaccccgg tccgcatgcg catcgaactt gctggccaag
      481 aaatacgcca atggcagtac cgggatatgt ggcccgcccg ctaatacgtg gctctcgcgc
      541 ttggggaacc cgctggcgta ccaacggttc cccggtgccc aaccgaccgg ctccagggcc
      601 atcacatcca ccatgatccc gtcgatccgg aagcggcaca taacggggtc ggcggcgctc
      661 tgcgtgaagc ccttcgctat caaccggccg cgcaaagtct ccagttctcc agcggtggcc
      721 accgagaccg cgaggtcgat atcgtacgtg ggccgggctt ccgaagcggc ggggtcgtcg
      781 gcatacacgg agaccacggc accgccaacg aaaacggccc gttccagatc cccacccagc
      841 gcttccgcca cacgctggag catctccagg ttcatcgggg cgttcgtggc catttcaacg
      901 aatgcgttcc cgcagttcct tcatggccag ctcccgctcg cgcacccggc ctgtgcgcag
      961 cgcatcggtg agcgccagca acgcgtacag gtccttgttc ttcgccgccg cttgcggaac
     1021 ggtggggtac aagggctgta tcgccgcacc gcgcgccttg cccatcggat cgggccaaac
     1081 gaagagggga ccgctcacga tggtctgctt caagggtggt gcggagtggg ctgtcgggac
     1141 accgcgaacc tcggccccgg gccttgccgg gaacacatac cgcacggcat ggtacagcat
     1201 gtccaggaac cctttggaca tcagctcccg gtcctcgctc agcaatccgc tctggatcga
     1261 ccgtttcagt gaatagccga tctccgccgg gctgatcacc aggtccgccg ccagatcctt
     1321 ggccttccac gtgccgcgct cacgcaacgc aatgctcaac aggaccacga tgtcctgtgg
     1381 tttcatgggg gcgggtccgg tgctggcgcg catgccgata ttggatttgc aaatccaaaa
     1441 ttaaacattc ttgctcacac gcgcatgggc atatacccac gtgcgcacat ggcctttcac
     1501 acctccacca acgtgcccac cttctcgccg gccaccacct tgcgcaggtt gccgcgcttg
     1561 ttcatgtcga agacgatgat gggcaggttg ttctcgcggc agagcgtgaa ggccgtgagg
     1621 tccatcacgc tgaggccctt gtcgtaggcc tcgtcgaagg tgatgcggtc gtacttcttc
     1681 gccttcttgt ccttctcggg atcggcggtg tagatgccgt ccacacgcgt gcccttgagg
     1741 atcacgtcgg cctcgatctc gatggcgcgc aaactggcgg cggtgtcggt ggtgaagtag
     1801 ggattgcccg tgccggcacc gaagatcacg atgcggccct tttccaagtg gcgcacggcc
     1861 cggcgacgga tgaagggctc ggcgatctgc tccatcttga tggcgctctg caggcgcgtg
     1921 ctctgcccct tggcctccag ggcgctctgc agggccaggc tgttgatcat ggtggccagc
     1981 atgcccatgt ggtcgccctg cacccggtcg ataccgctgc cgctggcgtt catgccccgg
     2041 tagatgttgc ccccgccgat gaccaccgcc acctgtgtgc cgcccttggc cacggcgatg
     2101 atctcgtcgg cgtaggcctt cagcatcgca tcgtcgatac cgtagctctt gccgcccatg
     2161 aggctttcgc cggagagctt cagaaggatg cgcttgtacg tgcgttccgt tttcgccttg
     2221 gccatgtggt ggggttcggg cccaaagcaa tgaaaaaggc gcgggccgag gtcaactccg
     2281 cgttgtgcct cagcgcaact tcaggaaccg ccgcgtgagg atgccgccgc ccgcgccctc
     2341 caaccgtacc
//
LOCUS       DAOQOC010000419         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27747_length_2344_cov_3.194845, whole genome shotgun sequence.
ACCESSION   DAOQOC010000419 DAOQOC010000000
VERSION     DAOQOC010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27747_length_2344_cov_3.194845"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1532)
                     /locus_tag="PL106_07525"
     CDS             complement(204..1532)
                     /locus_tag="PL106_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA32958.1"
                     /translation="MHPLTLALSLSAQPPLDNYIAQGLANNVVLQQKHIALDRAMQDL
                     KEAKSLFLPSVNLNASYLSGEGGRYFDFPVGDLVNPVYTTLNQFIGQERFPQVDNVQA
                     YLNPNNYYDAHVRASMPLLNTDIIHNRRIQQHAVQLQEYEVDIYRRELVKDIKVAYYN
                     VLMAAQAVRSYTSALQLLERNVEVTRSLHRHGSGVPAQVLRAQAEQEQITAQLAQATN
                     QYANAERYFNFLLNTDLDAPVDESLDVNAALGDTARTMAADISRREELQALRTAVGLR
                     DSQTKLTSNYWVPKLSTFVDLGYQGLDWSTDRYADYYLFGVQLDVPLFNGGRNRFKQH
                     RARSDRDFAELTERNSTQQLLLGAMQARTELATALAVLRSANARAAAAASYFNLIEKG
                     FNEGVNTQIEYLDARNQLTGAEVQLAVARTTVLQKLAEMERQHATYPLSK"
     gene            complement(1674..>2344)
                     /locus_tag="PL106_07530"
     CDS             complement(1674..>2344)
                     /locus_tag="PL106_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA32959.1"
                     /translation="PQACGINTVNYICALMNIAARKAQEKEGLKRAILNAARELFLER
                     GYDRTSMRMIAQRIAYSPTTIYIYFKDKNAIFHALHVEAFQQLGARLAPLAHVENPME
                     RLTAMGKVYLEFAEQNPGLYDLMFIMKAPVHAVDEMHHLWEEGDRVFAVLKATVEEAM
                     NKKLLRQSDVEVTSLLVWSTVHGMAALLVRDRCYQVIDPCKHPNLVKDSLDVFAQWLQ
                     SLKP"
BASE COUNT          409 a          665 c          760 g          510 t
ORIGIN      
        1 cgatggacgc acgtgcataa tggtcactgg tgtggcgcca ttttccgtgg tcccgttggt
       61 cgcatcggcg gagccgcatg cgctaaggag ggtgagcatg gtgagaatgg agaaaagggt
      121 aagaaagtga atgccgctcc accttttcac tttctctcca ccctcactac tctcaccact
      181 ctcaccaaag aatttcgttg tgctcatttg ctcaaggggt atgtagcatg ctgtctttcc
      241 atctcggcca gcttttgcag cacggtggtg cgggccacgg ccagttgcac ctcggcaccg
      301 gtcagttggt tgcgggcatc gaggtattcg atctgggtgt tcacgccctc gttgaaaccc
      361 ttttcgatca ggttgaagta actggccgca gcggcggcac gtgcattggc gctgcgcagc
      421 acggccaggg cggtggccag ttcggtgcgg gcttgcatgg ccccgagcag cagttgttgg
      481 gtggagttgc gctcggtgag ctcggcgaag tcgcgatcgg accgggcgcg gtgctgcttg
      541 aagcggttac gcccgccatt gaagaggggc acgtccaatt gcaccccgaa gaggtagtaa
      601 tcggcgtacc ggtcggtgct ccagtcgagg ccctggtagc ccaggtccac gaaggtgctc
      661 aacttgggca cccagtagtt gctggtcaat ttggtctggc tgtcgcgcag gcccacggcg
      721 gtgcgcaggg cttgcagttc ctcgcgcctg ctgatgtccg cggccatcgt tcgcgcggta
      781 tcgcccagcg cggcgttcac atcgaggctt tcatccaccg gggcatcgag gtccgtgttc
      841 aacaggaagt tgaagtagcg ttcggcattc gcgtactggt tcgtggcttg cgccagttgc
      901 gcggtgatct gctcctgttc ggcctgtgca cgcagcacct gagcgggcac accggagccg
      961 tggcgatgca acgaacgggt cacctccacg ttgcgctcca gcagttgcag ggcactggtg
     1021 tagctgcgca ccgcctgtgc ggccatcaat acgttgtagt aggccacctt gatgtccttc
     1081 accagctcgc ggcggtagat gtccacctcg tactcctgca actgcaccgc gtgctgttgg
     1141 atgcgccggt tatggatgat gtccgtgttc agcagcggca tgctggcgcg cacatgggca
     1201 tcgtagtagt tgttggggtt caggtaagcc tgcacattgt ccacctgggg gaaacgctcc
     1261 tgcccgatga actggttgag ggtggtgtac acggggttca ccaggtcgcc cacgggaaag
     1321 tcgaagtagc ggccgccttc gccgctgagg tagctggcgt tcaagttcac cgagggcagg
     1381 aagagcgact tcgcctcctt gaggtcctgc atggcccggt ccaaggcgat gtgcttctgc
     1441 tgcagcacca cgttgttggc cagtccttgg gcgatgtaat tatccaaggg gggctgggcg
     1501 gacagggaga gcgcgagggt gagagggtgc aagagtgcga gggagcgtgc aggaataccc
     1561 tcgcaccttc gcaccctttc gcccttcatc aggcatctac tgaacatcgt tcaatgtatt
     1621 tacaacgtta accacagggt cccaaaaaag cgaacggcac gcggtgtggc acatcacggt
     1681 ttgagggatt gcagccactg ggcaaagaca tccaggctgt ccttgaccag gttggggtgc
     1741 ttgcaggggt cgatcacctg gtaacagcgg tcgcgcacca gcagggcggc catgccatgc
     1801 acggtgctcc acaccagcag gctggtgacc tccacgtcgc tttggcggag cagcttcttg
     1861 ttcatggcct cctccacggt agccttgagc acggcgaaca cgcggtcgcc ctcctcccac
     1921 aggtggtgca tctcatcgac ggcatgcacc ggggccttca tgatgaacat gaggtcgtac
     1981 aggccgggat tctgctcggc gaactcgaga tacaccttgc ccatggccgt gagccgttcc
     2041 atggggtttt ccacgtgcgc gagcggtgcc agcctggccc cgagctgctg aaaagcctcc
     2101 acatgcaggg cgtggaagat cgcgttcttg tccttgaagt agatgtagat ggtggtgggg
     2161 ctgtacgcga tgcgctgcgc gatcatgcgc atgctggtgc ggtcatagcc ccgttccagg
     2221 aagagctcgc gcgcggcgtt caggatggcc cgtttcaggc cttccttctc ttgggctttt
     2281 cgtgccgcga tgttcatgag ggcgcaaata tagttaacgg tgttaattcc gcaagcctgg
     2341 ggat
//
LOCUS       DAOQOC010000420         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27882_length_2338_cov_3.755585, whole genome shotgun sequence.
ACCESSION   DAOQOC010000420 DAOQOC010000000
VERSION     DAOQOC010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27882_length_2338_cov_3.755585"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PL106_07535"
     CDS             <1..1307
                     /locus_tag="PL106_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA32960.1"
                     /translation="GRLPVTATSYFKQGMGSVLQSTHRPDFVLPEADGIRSGALRTVD
                     SIAWEGIRKGAYPGCQVVVVRDGHVLLNKAFGNTMYAGGRPVRTDDLYDIASVTKVVG
                     TTLALMKLYDEGKIDPDATLGTYLKDLSDEHPAHARLVLRDILTHQAGLAAFVPYYTK
                     LLRNGALRPDVFAVTQGPEYPVRVAEGVYMKAAYQDSLWAAMLAAPLKPRGTYLYSDI
                     DFHILARVVEAVSGERLDAFLERVFYRSMGLATIGYRPLERFPVDRIIPTEDDKSFRM
                     RLLRGDVHDPGAAMLGGVAGHAGLFSDATDLAAILQMLLNGGSYGGVEYLHQSTIDEF
                     TRCQFCAGTGPLNGENRRGLGWDKPQPVGKPGPACECVSLLSFGHTGFTGTQVWADPM
                     DRTITVFLSNRVHPSVNNRKLSDLNIRTRLQEAVHDAVAGRQ"
     gene            1774..>2338
                     /locus_tag="PL106_07540"
     CDS             1774..>2338
                     /locus_tag="PL106_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA32961.1"
                     /translation="MKQLSAFAIAIMLGSPAWAQTECIDGRYTDPAHFAEVTVTTAVT
                     FGSNIPVNGTTPVDLRMDIYSPIGDTLSGRPVVLVAFGGSFIGGARADVAPICEYLAH
                     RGYVAIAPDYRVGFFLPNANTTTLAVLRCAHDLRAAIRFLRKTVAEDGNPYHIDPERI
                     IVGGVSAGGIGAIHVAYLDQQSEIPPAI"
BASE COUNT          445 a          708 c          738 g          447 t
ORIGIN      
        1 gtggtcggct gcccgtgacg gccacatcat acttcaaaca gggtatggga agcgtccttc
       61 aaagcacgca tcgcccggat ttcgtgctcc ccgaagccga tggcatccgc agcggcgcgt
      121 tgcgcaccgt ggacagcatc gcatgggaag ggatccgcaa gggggcatat ccgggctgcc
      181 aggtggtcgt ggtccgcgac ggccatgtcc tgctgaacaa ggcgttcgga aataccatgt
      241 acgcaggtgg caggccggtg cgtacggacg acctgtacga tatcgcgagc gtgacgaagg
      301 tggtgggaac cacccttgcc ctgatgaagt tgtacgatga ggggaagatc gatccggatg
      361 cgaccttggg aacgtacctg aaggacctgt cggacgaaca tcccgcgcat gcccggttgg
      421 tgttgcgcga tatcctgacg caccaggccg gcctcgcggc attcgtaccg tattacacca
      481 agttgcttcg caatggtgcc ctgcgcccgg atgtgttcgc ggtcacccag ggtccggagt
      541 atccggtgcg ggtggccgaa ggggtgtaca tgaaagccgc ttaccaggac agcctgtggg
      601 ccgccatgct tgccgcgccg ctcaagccgc ggggcaccta tctgtacagt gacatcgatt
      661 tccacatcct cgcccgggtg gtggaagccg tgagcgggga gaggctggat gctttccttg
      721 agcgcgtatt ctaccggtcc atgggtctgg ccacgatcgg ataccgccca ctggaacgtt
      781 tcccggtgga tcgtatcatt ccgacggagg atgacaagag cttccgcatg cgcctgctgc
      841 ggggcgacgt gcatgatccg ggtgcggcca tgctgggtgg ggtggccggt catgccggcc
      901 tgttctcgga tgcgacggac ctggccgcga tcctccagat gctgctcaac ggaggctcat
      961 acggtggggt tgaatacctg catcaaagca ccattgatga gttcacccgc tgccagttct
     1021 gcgcgggaac cgggccgttg aacggggaga accggcgtgg actgggttgg gacaaaccgc
     1081 aacccgtggg caaacccggc ccggcctgcg aatgtgtgag cctgctcagc ttcgggcaca
     1141 ccggtttcac gggcacccag gtttgggccg accccatgga ccgcaccatc accgtcttcc
     1201 tgagcaaccg cgtgcatccc agtgtgaaca accggaaatt gagtgacctg aacatccgca
     1261 cccgcctcca ggaggctgtt cacgatgcgg tcgcaggaag acagtgagcg gaccacgagg
     1321 actgatcatt gctatccggc tttccgccac ttgctcgttc tggtacgcca cttgctcaac
     1381 agggtatacg ctcctctcca tcgcgcattt gttttgtcat cacaaatgat acggaaccca
     1441 ggcccgtaag cctggcgcac gaaggacaag accgggcccg taagcccggc acaagcaagc
     1501 aagttcaggc ccgtaagcct ggcgcacgaa ggacaagacc gggcccgtaa gtccggccac
     1561 agcaagccca agcccgtaag cttggtgcaa cagatcggtt ccgctgggaa gcggtccgtg
     1621 cgatccgagc cccgccccga gcggggctcg gtgttttggg cttcaggcat tcatgggggg
     1681 aaaccgggtc gacgtcgatg cgaggtggag cttgggcgac ggctatttga acgacgatgt
     1741 gctcacgtac tttgcgcgca ctgaacctga cacatgaaac aactttccgc cttcgcgatc
     1801 gcgatcatgc tcggatcccc ggcatgggct caaaccgaat gcatcgatgg ccgttatacc
     1861 gatcccgccc atttcgcgga agtaacggtc accaccgcgg tgaccttcgg cagcaacatc
     1921 cccgttaatg gtaccacacc tgtggacctg cggatggaca tctactcgcc tatcggcgat
     1981 accttgtccg gaagaccggt cgtcctggtg gctttcggag gttccttcat cggcggggcc
     2041 cgggccgatg tggccccgat ctgcgagtat ctcgcgcaca ggggttacgt ggcaattgcc
     2101 cctgattatc gcgtaggatt tttcctcccg aacgccaaca cgaccaccct ggccgtgttg
     2161 cgttgtgccc atgatctgcg tgctgccatc cggttcctgc gcaaaacggt ggccgaggac
     2221 gggaatccct accacatcga tccagagcgg atcatcgtgg gtggcgtgag tgcggggggc
     2281 atcggtgcga tccatgtggc ctacctggac cagcagagcg agatcccgcc ggcgatat
//
LOCUS       DAOQOC010000421         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27890_length_2338_cov_2.759965, whole genome shotgun sequence.
ACCESSION   DAOQOC010000421 DAOQOC010000000
VERSION     DAOQOC010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27890_length_2338_cov_2.759965"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="PL106_07545"
     CDS             complement(<1..2148)
                     /locus_tag="PL106_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32962.1"
                     /translation="MTAASDAGSDGAILLCSASPPTDLLTHLNGTPQTDGTWSDPDGT
                     PSNGSFDPANDAPGTYTYTVPGTSPCPNASATVLVNVSSDPDAGLPGETTLCSIAGMV
                     DLLDLLEGTPDAGGAWTGPDGAPHPDLLDPVTDPPGTYSYLIAVPPPCTSASSTVTID
                     FVAPADPGLDNTVVHCATDPSFDLFAQLGGSPDTDGAWSLDGQPQDSLFNPGIDTPGN
                     YLYSVISVAPCPAVTAVVQVIVNVPPDPGVNGAAALCASGDPIPLVGLLGGTPQSGGT
                     WSFDGISHGDLIDPSSDQPGTYSYTVLGNAPCPAEVATVDIAIATEPDPGTSGLVTVC
                     SSGAPIDLFANLGGTPDPGGSWSGPSPSTEGLFDPNSMQPGIYTYLLDVPPPCTSASS
                     TVTVMAVIPPDAGSDSDLLLCASSPITGLIDVLGGDPQAGGGWFAPNGAAHGPFFNPD
                     NDPIGTYVYIVNGMAPCPSDSALATLSVVSSPDPGSNGFLSQCWTDPPEDLFERLEGT
                     PEPGGSWSGPSPCPDGFFHASTMLPGTYFYLLAAPPPCPDTSSNVLVSVSLPPDPGTD
                     GAVTLCTTNAVTDLFAQLNGTPQSGGAWTAPDGSPHPGSFLPAQDPAGIYTYTVQGND
                     PCPAEAATVSMTLHTPPDAGLDGDLTLCPEAAVVDLFTQLGGLPDAGGTWALVGGGAD
                     DGIFDPPTDQSGSYTYTVQGMAPCADDTAHVMVI"
BASE COUNT          430 a          694 c          801 g          413 t
ORIGIN      
        1 gatcaccatc acgtgggccg tatcatcggc acaaggcgcc atcccttgaa ccgtgtatgt
       61 atagctgccg gattggtcgg ttggcggatc gaaaatgcca tcatccgcac cgcctccgac
      121 aagggcccat gtgccgccag catcgggcag tcctccaagc tgggtaaaaa ggtccaccac
      181 ggccgcttcc gggcacaggg tcaagtcacc atcgagtccc gcatcaggcg gcgtatggag
      241 ggtcatgctc acagtggctg cctcggccgg acagggatca ttgccttgga ccgtatacgt
      301 atagatgccg gccgggtcct gtgccggcag gaatgatccc ggatgggggg aaccatccgg
      361 cgccgtccac gcacctccgc tctgcggcgt tccattgagc tgcgcgaaga gatccgtgac
      421 cgcattggtc gtacacagcg tgacggcccc gtccgttccg ggatcaggag gaagggacac
      481 gctgaccagc acattgctgc tggtatccgg acagggtggt ggagcggcga gcaggtagaa
      541 gtaggtgcct ggcagcatgg tgctcgcgtg gaagaaacca tccgggcagg ggctggggcc
      601 gctccacgaa ccaccgggtt ccggtgtgcc ttccaaccgt tcaaaaagat cctcgggtgg
      661 atcggtccag cactgcgaga ggaagccatt gcttccgggg tccggtgaac tcaccaccga
      721 cagcgtcgcg agggcactgt cggacggaca aggcgccatg ccgttcacga tgtacacgta
      781 cgttccgatc ggatcgttgt ccgggttgaa gaaagggccg tgcgcagcgc cgttcggggc
      841 gaaccagccg ccgcccgcct gtgggtcacc gcccagcaca tcgatcagcc cggtgatcgg
      901 tgaactcgcg caaaggagga ggtcactgtc cgaaccggca tccggtggga tcacggccat
      961 cacggtcacg gtactgctcg cactggtgca tggcgggggt acatcaagca ggtaggtgta
     1021 tattccgggc tgcatcgagt tcgggtcgaa cagcccctcc gtggaaggac ttgggccgct
     1081 ccatgagcca ccggggtccg gtgtaccgcc caggttggcg aagaggtcga tgggggctcc
     1141 gctcgagcaa acggtgacca gtccgctcgt tccgggatcg ggttcagtgg cgatcgcgat
     1201 atccaccgtg gccacctcgg ccggacatgg tgcgttgccg agcaccgtgt aactgtacgt
     1261 cccgggttga tcgctggatg gatcgatcag gtcaccgtgg ctgatgccgt cgaaggacca
     1321 agtgcctccg ctttgcggcg ttccaccgag cagaccgacc aaggggatcg ggtcgccgct
     1381 ggcacagaga gcagcggcac cgttgacccc cgggtcgggt ggcacgttga cgatcacctg
     1441 caccaccgcg gtcaccgccg gacatggagc aacactgatc acggaataca agtagttgcc
     1501 tggcgtgtcg atgccggggt tgaacaggct gtcctgtggc tgcccgtcaa ggctccaggc
     1561 tccatcggta tcgggtgatc caccgagttg cgcgaagagg tcgaacgacg ggtcggtagc
     1621 gcagtggaca acggtattgt cgagcccggg gtcggcgggc gccacgaagt cgatggtgac
     1681 cgtgctgctc gcactcgtac acggaggagg caccgcgatg aggtatgaat acgtgccggg
     1741 aggatcggtc acgggatcca gcaggtcggg atgcggcgca ccatcgggac cggtccaagc
     1801 gccgccggca tccggggtcc cctccagcag gtccaacagg tccaccattc ctgcgatcga
     1861 gcagagggtc gtttcgccgg ggagtccagc atccgggtcc gatgaaacgt tcacgagcac
     1921 tgtggcgctc gcattggggc agggtgaagt tcccggaacg gtgtaggtat atgtaccggg
     1981 tgcatcgttg gcagggtcaa agctgccgtt cgacggggtg ccgtccggat cgctccatgt
     2041 gccatcggtc tgtggcgtac cgttcagatg ggttaacaga tcggtgggcg gactggccga
     2101 gcagagcagg atcgcgccat cagagccggc atccgaggct gcggtcatgc tcacatcgat
     2161 caccgcctgc gcattcgggc aaggggatgt tcccgcaacg aggtatgtat atgtcccggc
     2221 ggggtccgaa cccgggtcaa gagagccagg gaatggtgat ccgcccggtg ctgtccaggt
     2281 gccgttcggt tccggcgtcc ctcccagtgc ctcgaaaagg tcgacgatgc tgttcgtg
//
LOCUS       DAOQOC010000422         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27924_length_2336_cov_3.558965, whole genome shotgun sequence.
ACCESSION   DAOQOC010000422 DAOQOC010000000
VERSION     DAOQOC010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27924_length_2336_cov_3.558965"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PL106_07550"
     CDS             <1..1159
                     /locus_tag="PL106_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32963.1"
                     /translation="LFDRSQACGSGAMMMMASGGANAAEQTMNAANESTTLKAAYDDW
                     TDGGDTEGLLDFVVNPANDSYHIRNELMLVAPKVSSDIWKLVFTDRFADMNPWHLAQA
                     LIANSPLEPEVYRMMDESDLTPFYKQLVSGEQGGGMNMQTIMESELAYWMSEQSEALT
                     VYTALAIDEDPTVSIADAIAMHQQYPVPGSDQQIYLLHLANNDVSAAQNDLNTALAND
                     HDAWWDVQAIHLTKVIAEEELESLSVTQESTLHGIAEGEGPGASAAAAWLATSLGERP
                     VTEVILPGTGTRSFMGGRAASGALEPNWLVVYPNPTKGDAYLTYRIPEGATVGAIEVR
                     DATGRIVRTRNLRGAGGIEELPKSVLPPGLYTVALRADGILVGAVKFISVR"
     gene            1709..>2336
                     /locus_tag="PL106_07555"
     CDS             1709..>2336
                     /locus_tag="PL106_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32964.1"
                     /translation="MKTDSLGNVQWWQTYGHPTRSDIATSVDHAPDGGYYIGGDYLAT
                     PDGKVQWIVRTDSVGNLIWQVWDGQPVEATFSAAVLATFDGNVVYAAGKYSGGSDQEH
                     WPHLAKLDTAGNTIWDQTYGTAFYGTGFFSVAEITDSHDLIACGQRYYVNNLNIPYSK
                     GILLRANSQGDSLWMREYAYFDSTVTDCEGTLRDVQPTPDGGFVAVGTT"
BASE COUNT          517 a          642 c          714 g          463 t
ORIGIN      
        1 cctcttcgat aggagccagg catgcggatc cggggctatg atgatgatgg ccagtggtgg
       61 cgccaacgcg gccgagcaga cgatgaacgc ggccaatgaa agcactacct tgaaggccgc
      121 atacgacgat tggaccgatg gcggcgacac ggagggatta ttggatttcg tggtgaaccc
      181 ggcgaacgac agctaccaca tccgcaatga actgatgctg gtcgcgccca aagtgagcag
      241 cgacatctgg aagctggtct tcacggacag gttcgctgac atgaatccct ggcacctggc
      301 gcaagcactc attgcgaaca gcccgcttga acctgaagtc taccgcatga tggacgagag
      361 cgacctcacc cccttctaca aacaactggt gtccggtgag cagggtggcg gcatgaacat
      421 gcagaccatc atggaaagcg aactggccta ctggatgagc gagcagagtg aagcgctgac
      481 cgtgtacacc gcccttgcga tcgatgaaga ccccaccgtg agcatcgccg atgccatcgc
      541 catgcatcaa cagtatccgg tgccggggag cgaccagcag atctatctgt tgcatctcgc
      601 aaataacgat gtgagcgccg cacagaatga cctgaacacc gctctcgcca atgaccatga
      661 tgcatggtgg gatgtgcaag ccattcacct aacgaaagtg atagcggagg aagagttgga
      721 atcgctgtcc gttacccaag agagcaccct tcacggcatc gcggaaggag aaggccccgg
      781 cgcttcggcg gcagcagcat ggctggcaac ctccctgggt gagcgccccg tgaccgaggt
      841 gatcctgccg ggtaccggca cccgatcgtt catgggagga cgcgcggcct ccggcgcgtt
      901 ggagcctaac tggctggttg tataccccaa ccccacgaaa ggcgacgcgt acttgaccta
      961 tcggatccct gaaggtgcca ccgttggagc catagaggtg cgcgatgcga ccggtcgcat
     1021 cgtgcgcacg cgcaatctcc gcggtgctgg tggcattgaa gagttgccga agagtgtgct
     1081 accgccagga ttgtacacgg tggccttgcg ggctgatggc atcctcgtgg gtgccgtgaa
     1141 gttcatcagc gtgcggtagc tcatgctacg atcaactggc aaagttggtc ttgcgatcgt
     1201 agtgcagggg agcatgtgct cccctgcacc agcgcaagat ttcaatgtgc ggcacgacct
     1261 gttcggttgg caacaagcgc gtgctgcata tagcgcggag caaacagcgg ctggtgatct
     1321 gttggtcttc ggcaacggac catggctgga cagcgcgacg atgcttacct atagctcggt
     1381 gtgcagtgtt gagcgctttg atgcttcggg tgatttcctg gcacaagaca ccgcctttct
     1441 tgaccttcag gcgagctaca taggctggtc caattgttca accagcttgc ccgatggtcg
     1501 ggtcgtggta tccggtgggg tgacggatgg accggacacg aatagggcgt ccatctattg
     1561 gttctcttca actggtatgt tggtccacag tgtgcaacta ctcccgatat ccaggtcgtg
     1621 gattgcttat cagcacaaga ataccagtga cggaggtttc attttgacag gtgtaacaag
     1681 cgccaccggt gttcaggaca ttttcctctt gaaaaccgac agcctgggga acgtgcagtg
     1741 gtggcagacc tacgggcacc cgacacggtc ggacattgcg acctcggtcg atcatgctcc
     1801 cgatggtggt tattacatcg gtggggatta tctggcaacg cccgatggga aagtgcaatg
     1861 gatagtgaga acggacagtg ttggtaacct gatctggcaa gtatgggatg gccagccggt
     1921 agaagccaca ttcagtgctg cggtacttgc cacattcgat ggcaacgtgg tgtacgcggc
     1981 cggcaagtac agcggtggca gcgaccaaga acactggcct cacctggcca agctcgacac
     2041 ggccggcaac accatctggg accagaccta cggcaccgct ttctatggta caggcttctt
     2101 ttccgtggcc gaaataaccg acagccacga cctgatcgcc tgcggtcaac ggtactatgt
     2161 gaacaacctg aacataccct atagcaaagg gatattgctg cgggcgaata gccagggtga
     2221 cagcctgtgg atgcgggagt atgcctactt cgacagcacc gtcaccgatt gcgaaggcac
     2281 cttgcgcgat gtgcagccca caccggatgg gggctttgtg gccgtaggca cgacct
//
LOCUS       DAOQOC010000423         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_27937_length_2335_cov_3.896930, whole genome shotgun sequence.
ACCESSION   DAOQOC010000423 DAOQOC010000000
VERSION     DAOQOC010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27937_length_2335_cov_3.896930"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1906)
                     /locus_tag="PL106_07560"
     CDS             complement(329..1906)
                     /locus_tag="PL106_07560"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA32965.1"
                     /translation="MDRRRFLRATSIATIGGMSVRGFSSPLLRALGSRDLGDRVLVVV
                     QLYGGNDGLNTVIPLDQYGILSSVRPHVIIPESAVLPLSGLDGTGLHPALGGLQSMWN
                     DGKLSIVQGVSYPEPNYSHFRATDIWETGADHDQILNSGWTGRYLNLEYPNYPSGYPN
                     AEMPDPLAIRIGGPVGPALQYLGLSMGTAIVNTDDPLDLSGNIYNDPLSALCSGNKLG
                     LVRTVQEQTDLYGDVINAAAQMGCAHGPYPEGTTPGAKLGQALRIVAQLICGGLKTRI
                     YWVSLTGFDTHAEQVQESDHTLGTHANLLQGLNNAIHAFQNDLAGLGLEDRVLGMTFS
                     EFGRRIVSNASIGTDHGSAQPMFLFGSKVIPGMLGTNPEIPVDTSVQTNLAMQYDFRS
                     VYASILTDWFCLQPEETDQVLLDTYQPLALVDPEGCINIGVHEQNQRAGDGLLSVYPS
                     PFTDRITVNYVANGGRVLVQVYNESGKLMTTLLNAPMAPGNHTLSADLGELPAGVYYC
                     RLQNESRQQVRNILKVR"
     gene            complement(1911..>2335)
                     /locus_tag="PL106_07565"
     CDS             complement(1911..>2335)
                     /locus_tag="PL106_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32966.1"
                     /translation="QYDEIWMDTTSYPARSNTLAGILFAGFTTDGNMYEPSSQNLEFK
                     ADLLAVVAQFSDPADPNVLVQEAADLLFAVPISAAVRDQLKSSFLLFGQTNDLYWTYA
                     YTTYVADPQTTDMAAQLVPVMLQALFNDMRGAAEIQLF"
BASE COUNT          410 a          697 c          768 g          460 t
ORIGIN      
        1 ccgtagcggc tacgcaaagg cgcggtcgac gggctcaccg tgacattcaa taggacttac
       61 gacggaggtc gaatatcgct atttctgggt attgcatgac ccgatagggg tgcggaacat
      121 tgcaccgcag atcggatggc cctggtggag cggggctgtc acctgaacga cccagcgatc
      181 ggcttggagc acggccacct ggtagcggcc cctcgtgaac gaaggttcac ggggggcttt
      241 cgttccggga tccaggatgc cggtcccgcc gcaacaaggt tctccgcccg atcctcctcc
      301 catgaacagg ggccaggagc tgatccgctc agcgaacctt gaggatgttc cgcacctgct
      361 ggcggctctc gttctgtaaa cggcagtagt agacgccagc aggcagctct cccagatcgg
      421 cactcagggt atggttgcct ggagccatgg gggcgttcag aagggtggtc atcagcttgc
      481 cgctctcatt gtacacctgc acgagcacac ggccaccgtt tgccacgtag ttcactgtga
      541 tcctgtcggt gaacgggctc gggtacacgc tgagcaatcc gtcccctgcg cgctggttct
      601 gctcatgcac gccgatgttg atgcatccct ccggatcgac cagtgccaac ggctggtagg
      661 tgtccaacag tacctgatcg gtctcttcgg gctgcaggca gaaccagtcg gtcagtatgg
      721 aggcgtacac cgatcggaaa tcatactgca tggccaggtt ggtctgcacc gaggtgtcca
      781 ccgggatctc cgggttggtc cccaacatgc cggggatcac cttgctgccg aagaggaaca
      841 tgggttgggc actgccgtgg tcggtgccga tgctcgcatt gctcacgatg cgccgtccga
      901 attcgctgaa ggtcatgccg agcaccctgt cctccaggcc caagccggcc agatcattct
      961 ggaaagcatg gatcgcattg ttgaggcctt gcaacaagtt ggcgtgcgtg cccagtgtgt
     1021 ggtcgctctc ctgcacctgt tcggcatgcg tatcgaaccc ggtaaggctt acccaataga
     1081 tgcgggtctt gaggccaccg cagatcaatt gggccacgat ccgcagggcc tgccccagtt
     1141 tggcgcccgg ggtggtccct tcgggataag gcccatgggc gcaacccatc tgcgcggccg
     1201 cgttgatcac gtcgccgtac aggtcggtct gctcctgcac ggtgcggacc aggccgagtt
     1261 tgttcccgct gcagagggcg ctgagcgggt cgttgtagat gttgccggac aggtcgagcg
     1321 ggtcgtcggt gttcacgatg gctgtaccca tgctcaaccc cagatactgc aatgcgggtc
     1381 ccacgggacc gccgatgcgg atcgccagcg ggtcgggcat ctccgcgttc gggtatcccg
     1441 atgggtagtt cgggtactcc aggttcaagt agcggcccgt ccagccgctg ttcaatatct
     1501 ggtcgtgatc ggccccggtc tcccagatgt ccgtggcgcg gaagtgcgag tagttcggtt
     1561 cgggataact gacgccctgc acgatggaca gcttgccatc attccacatg ctctgcaagc
     1621 cccccaaggc ggggtgcagc ccggtgccgt ccagcccgct cagggggagc acggcgctct
     1681 cgggtatgat cacgtgcggc cggacggagg aaaggatacc gtactggtcc aacgggatca
     1741 ccgtgttcaa tccgtcgttg ccaccgtaca actgcaccac cacgagcacg cggtcgccca
     1801 ggtcgcgcga gcccagtgcc cgcaacaagg ggctgctgaa gccgcgcaca ctcatgccgc
     1861 cgatggtggc gatggatgtg gcgcggagga atcgtctgcg gtccatggct tcagaacagt
     1921 tgtatctcgg ccgctccacg catgtcgttg aacagggctt gcagcatcac gggaacgagc
     1981 tgcgcggcca tgtcggtggt ctgcgggtcg gccacgtagg tggtgtacgc gtaggtccag
     2041 tacaggtcat tcgtctggcc gaagagcagg aaggatgact tcaattggtc gcgcacggca
     2101 gcggatatgg gcacggcgaa aagcaggtcg gcggcctcct gcaccagtac gttcgggtcc
     2161 gcggggtcgc tgaactgggc gaccacggcg agcaggtcgg ccttgaattc caggttctgg
     2221 ctggacggtt cgtacatgtt cccgtcggtg gtgaacccgg cgaagaggat gccggccaac
     2281 gtgttgctgc gcgcggggta cgaggtggtg tccatccaga tctcgtcgta ctggg
//
LOCUS       DAOQOC010000424         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28016_length_2332_cov_3.913043, whole genome shotgun sequence.
ACCESSION   DAOQOC010000424 DAOQOC010000000
VERSION     DAOQOC010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28016_length_2332_cov_3.913043"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..565
                     /gene="greA"
                     /locus_tag="PL106_07570"
     CDS             92..565
                     /gene="greA"
                     /locus_tag="PL106_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNA32967.1"
                     /translation="MSTPAYYTEEGFKKLQEELHRLKTVDRPHISQQIADARDKGDLS
                     ENAEYHAAKEDQGLLEARIAKLEEVMASARIVDADQMDTSKVYIHCTVKVRHTITDTH
                     KNFTLVAESESDIKVGKISVSSPIGKGLLGKMVGEIAEVLTPAGSTRFEIIEITR"
     gene            570..959
                     /locus_tag="PL106_07575"
     CDS             570..959
                     /locus_tag="PL106_07575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNA32968.1"
                     /translation="MPSIFTRIIHGEIPCHKVGEDERFLAFLDINPLREGHTLVIPKI
                     EVDKFFDLPTDVLSGIMPFAQGVAQRIARVVPCDRVGVSVVGLEVPHAHVHLIPLDRV
                     SDMDFNRPKLSVTPEQLAAIAERIRNV"
     gene            complement(968..1546)
                     /gene="ruvC"
                     /locus_tag="PL106_07580"
     CDS             complement(968..1546)
                     /gene="ruvC"
                     /locus_tag="PL106_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNA32969.1"
                     /translation="MALPSERIILGIDPGTTVMGFGVLLVEGQRLRLLEAGTIRFPAE
                     DDHTLKLRAIFTGTLDIIDRHHPDELAIEAQFFGKNVQSMLKLGRAQGVAIAAALQRE
                     LAVVEYAPKRVKQSITGNGNATKEQVAAMLLSILGVKELPASTDATDAVAVAVCHHFG
                     AGPNAGGARKGRGAKGGGWAGFVRANPERIKG"
     gene            1577..>2332
                     /locus_tag="PL106_07585"
     CDS             1577..>2332
                     /locus_tag="PL106_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNA32970.1"
                     /translation="MSKRTLLVLRIAVFIAACAFLYVRLGRDGAGLQRLFAWFTYAHS
                     PWSALVPVVLLMLLNWSIEALKWRVLVREVERMPFVRAFAAVVAGTSIGLITPNRVGE
                     FAGRVLFLRPDHRVRGAFATLLGSIAQFVVTVLVGALFLFLFPLDFVDQEPGALWPVV
                     RWCALLIGGATVFLYFSPGTLERVFLAVPWSRRFERQVRVLGAFGGKRLVVVAALSAL
                     RYAVFTLQFVWLLHVTGAVPWTSAFARVPVIFLI"
BASE COUNT          384 a          741 c          727 g          480 t
ORIGIN      
        1 tgagcctgta agtcgcttat ctttgagcga ggatccccgc ccggcgggga tcctcgcctt
       61 ttcacatcta cctgccccaa cgtcagaacc catgagcacc ccggcctact ataccgagga
      121 aggcttcaag aagctgcagg aggaactgca ccgcttgaag accgtggacc gaccgcacat
      181 cagccagcag atcgccgacg cacgcgacaa gggcgatctg agcgagaatg ccgaatacca
      241 cgccgccaag gaggatcagg gtctgttgga ggcccgtata gccaagctgg aggaggtgat
      301 ggccagtgcc cgtatcgtgg atgccgacca gatggacacc agcaaagtgt acatccactg
      361 tacggtgaag gtgcgccaca ccattaccga cacgcacaag aacttcacct tggtggctga
      421 aagcgaatcg gacatcaagg tgggcaagat cagcgtgagc tcgcccatcg gaaaaggcct
      481 gttgggcaag atggtcggcg agatagccga ggtcctcacc ccggcgggca gcacccggtt
      541 cgagatcatc gagatcaccc gctgagccga tgcccagcat cttcacccgg atcatccatg
      601 gcgagatccc ctgccacaag gtgggcgagg acgagcggtt cctggctttc ctggacatca
      661 acccgctacg cgaaggccat acgttggtga taccgaagat cgaggtggac aagttcttcg
      721 atctgcccac cgatgtgctc agcggaatca tgcctttcgc gcagggcgtg gcccagcgca
      781 tcgcacgggt ggtaccctgc gatcgggtcg gggtgagcgt ggtgggcctg gaggtaccgc
      841 atgcgcatgt gcatttgatc cccttggacc gggtgagcga catggacttc aacaggccga
      901 aactttcggt cacgcccgag caactcgcgg ccattgccga gcgcatccgg aacgtctgat
      961 ccgtccgtca gcccttgatc cgttccgggt tcgcgcgcac gaaacccgcc caacccccac
     1021 ctttcgcacc gcgccctttg cgtgcacctc ccgcattcgg accggccccg aaatgatggc
     1081 aaacggccac cgctaccgcg tcggttgcat cggtgctggc ggggagttcc ttcacgccga
     1141 ggatgctcag caacatggcg gccacctgtt ccttggtggc gttcccattg ccggtgatgc
     1201 tctgcttcac gcgtttcggg gcgtattcca ccacggccag ttcgcgctgt agcgccgccg
     1261 cgatggccac accctgcgct cgccccagct tgagcatgct ctgcacgttc ttgccgaaga
     1321 attgcgcttc gatcgccagc tcgtccgggt gatggcggtc gatgatgtcc agtgtacccg
     1381 tgaagatggc gcgcaacttc agggtgtggt catcctccgc cgggaaccgg atcgtgcccg
     1441 cctccagcaa ccgcaatcgc tgcccctcca ccagcagcac gccgaagccc atcaccgtgg
     1501 tgccggggtc gatgccgagg atgatgcgtt cggagggaag ggccacccgg gtacttttga
     1561 tcgctgcgaa gctaccgtga gcaaacgcac cctcctggtc ctgcggatcg ccgtgttcat
     1621 cgcggcctgt gccttcctgt acgtgcgtct gggtcgtgat ggagctggtt tgcaacgtct
     1681 gttcgcctgg ttcacctacg cgcactcgcc ctggtcagca ttggtgcccg tggtcttgct
     1741 catgctgctg aactggagca tcgaggcgct gaaatggcgt gtgttggtgc gcgaggtgga
     1801 gcgcatgccc ttcgtgcggg ccttcgccgc tgttgtcgcg ggcaccagca tcgggctgat
     1861 caccccgaac cgcgtgggcg agttcgccgg acgtgtgctc ttcctgagac ccgaccaccg
     1921 cgtacgcggg gcctttgcca ctttgctcgg cagcatcgcg cagttcgtgg tcaccgtgct
     1981 ggtcggtgct ttgttcctct tcctttttcc attggacttc gtggatcagg agccgggtgc
     2041 gctgtggccc gtagtgcgct ggtgcgcgct gctgatcggt ggggccacgg tcttcctgta
     2101 cttcagtccg ggtaccttgg agcgggtctt cctggctgtt ccctggtccc gccggttcga
     2161 acggcaggta cgtgtgctcg gcgcgttcgg cggcaaacga ctggtggtgg tggccgcgct
     2221 gagtgcgttg cgctacgccg ttttcaccct ccagttcgtt tggttgttgc atgtgaccgg
     2281 cgcggtaccc tggacctcgg cgttcgctcg tgtgcccgtg atcttcctca tc
//
LOCUS       DAOQOC010000425         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28149_length_2326_cov_4.464553, whole genome shotgun sequence.
ACCESSION   DAOQOC010000425 DAOQOC010000000
VERSION     DAOQOC010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28149_length_2326_cov_4.464553"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PL106_07590"
     CDS             complement(<1..793)
                     /locus_tag="PL106_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNA32971.1"
                     /translation="MSSGGPSLSEVHSTVAIPAGRGRWRRVMAFLGPAYLVSVGYMDP
                     GNWATDIAGGSAYGYRLIWVLLMSNLMALLLQSLSARLGIVRGLDLAQASRHTYPPVV
                     NIPLYLLAEVAIAACDLAEVVGLAIGLNLLFDIPLLYGVLISGLDTLLILLLLDRGMR
                     AMEVFIVSVVSLIGAAFLAEMFIVQPDVGAVMRGFVPSSLSGQALYIAIGIIGATVMP
                     HNLYLHSSLVQTRRFERTESGRREAIRFNFMDTAIVPYIVRDGYAN"
     gene            complement(790..1473)
                     /locus_tag="PL106_07595"
     CDS             complement(790..1473)
                     /locus_tag="PL106_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNA32972.1"
                     /translation="MLTRSEEDHLKAVYALLQHSDQENGRNRTASTKAIAERLSTKAS
                     SVTDMLKKLAEKGLLKHVPYYGVKLSAKGQAAALRLVRKHRLWETFLVERLGFGWNEV
                     HEVAEQLEHVSSDKLTNSLEEYLGHPRFDPHGDPIPDRDGNVQVRSTRPLTRCVVGER
                     VRLAAVKGPNDALLQLLDQKGIGIGMVYHVRSRQVFDGSIELRSKEGQESTVSEQVAA
                     QLLVEPVRP"
     gene            1536..>2326
                     /locus_tag="PL106_07600"
     CDS             1536..>2326
                     /locus_tag="PL106_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNA32973.1"
                     /translation="MRTLGPLMIPLLAGCAAMEPSAPADHELLDGPIAGLSSEELLRF
                     HRGDQAFNEEVFHAGNGLGPVFVATSCGSCHVGDGPGHPFTTLTRFGQADANGNQYLD
                     QGGPQLQHRAVPGVQPESLPVDAPSARFLPPLNTGLGFLDAVPDAEILAWADEFDADG
                     DGISGRPNLLQRKTYSPLRAGSVEVDGLVVGRFGRKASVYDLLQQTASAYNQDIGVVS
                     AFEPLDPYSQEPQDPEVATTTVHDVVFYLRTLKAPVQRDAGRPAVV"
BASE COUNT          431 a          741 c          716 g          438 t
ORIGIN      
        1 ggttggcata gccgtcgcgc acaatatagg gcacgatggc ggtgtccatg aagttgaaac
       61 ggatcgcctc ccgtcggcca ctttcggtcc gctcgaagcg ccgggtctgt acaagggagc
      121 tgtggaggta gagattgtgc ggcatcaccg tggccccgat gatcccgatg gcgatgtaca
      181 gcgcctgacc cgagagcgac gacggtacga agccccgcat caccgcgccg acatccggtt
      241 gcacgatgaa catctcggcg agaaaggccg ctccgatgag gctgaccacc gatacgatga
      301 agacctccat cgcccgcatg ccacggtcca acaggagcag gatcagcagg gtgtccagcc
      361 ctgagatgag cacaccgtac agcaggggaa tgtcgaaaag caggttcagg ccgatggcca
      421 ggcccaccac ttcggccagg tcgcatgccg caatggcgac ctccgccaga aggtagagcg
      481 gaatattcac caccggcggg tatgtatggc gggaggcttg ggcgaggtcg agtcctcgga
      541 cgatgccgag ccgcgcgctg aggctctgca gcagcagggc catcaggttg ctcatcagca
      601 gcacccagat caggcggtaa ccgtacgcgc ttccaccggc gatgtccgtg gcccagttgc
      661 cgggatccat gtagcctacg ctcaccagat aggccggtcc caggaaagcc attacccgtc
      721 gccagcgtcc acgacccgca gggatcgcca ccgtgctatg cacctcgctg agcgaaggac
      781 ctccggaact catgggcgca cgggttcaac cagcagttgt gccgccacct gttcggatac
      841 ggtgctctcc tggccctctt tcgacctcag ttcgatgctg ccgtcgaaga cctgccgcga
      901 ccttacatgg tacaccatcc cgataccgat gcctttctgg tccaacaact ggagcaacgc
      961 gtcgttcgga cctttcactg cggcaaggcg cacgcgctcg cccacgacgc atcgggtcag
     1021 cggtcgtgtt gatcgcacct gcacgttgcc gtcacgatcg ggaatgggat caccatgcgg
     1081 gtcgaagcgc gggtggccca ggtactcctc caggctgttc gtgagtttgt cgctgctgac
     1141 gtgctccaac tgctcggcca cctcatgcac ctcgttccag ccgaagccca accgctccac
     1201 caggaaggtc tcccacaggc gatgcttgcg caccaggcgc aaggcggcag cctgtccttt
     1261 cgccgagagc ttcacgccgt aatacggcac gtgcttcaac aagcctttct cggccagttt
     1321 cttcaacatg tcggtcacgc tgctggcctt ggtgctcagg cgctccgcga tggccttggt
     1381 ggaggcggtc ctgttccggc cattctcctg gtcgctgtgc tgcaggagtg cgtacaccgc
     1441 tttcagatga tcctcttcgc tccgggtgag catgatgggg caaaagtaaa caaaatatta
     1501 gacatgccta atttattatt tacttcaccc ccgaaatgag gacgctgggc ccgttgatga
     1561 tcccgctgct ggcggggtgt gccgccatgg agccttccgc cccggccgac catgagttgc
     1621 tggacggacc gatcgcggga ctttcttcgg aggaactcct gcgtttccat cgtggcgacc
     1681 aggccttcaa cgaggaggtc ttccatgccg ggaacggcct ggggccggtt ttcgtggcca
     1741 cgagctgcgg gagctgccat gtgggcgacg gaccggggca tccgttcacc acactgaccc
     1801 gtttcggaca agcggacgcc aacgggaatc aatacctgga tcaaggtggt ccacaactcc
     1861 agcatcgggc cgttcctggc gtgcagcccg aatcgctgcc cgtggacgcg ccgtccgccc
     1921 gtttcctgcc gcccttgaac acgggtcttg gtttcctgga tgccgtgccg gacgcggaga
     1981 tcctggcctg ggccgacgag ttcgatgcgg atggcgacgg gatcagcggc agaccgaatc
     2041 ttctgcaacg aaaaacatac tccccgttgc gtgccggttc ggtcgaggtg gatggcctgg
     2101 tggtcggtcg gttcgggcgc aaggcctcgg tgtacgacct gttgcagcag accgcttcag
     2161 cctacaacca ggatatcggg gtggtcagtg cctttgaacc gctggatccg tacagtcagg
     2221 aaccacaaga ccccgaggtg gccaccacga ccgtacacga cgtggttttc tacctgcgca
     2281 ccctgaaggc acccgttcag cgcgatgcgg gccggccggc cgtggt
//
LOCUS       DAOQOC010000426         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28191_length_2324_cov_4.817100, whole genome shotgun sequence.
ACCESSION   DAOQOC010000426 DAOQOC010000000
VERSION     DAOQOC010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28191_length_2324_cov_4.817100"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PL106_07605"
     CDS             <1..989
                     /locus_tag="PL106_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA32974.1"
                     /translation="WKPSEKTPICGVVCQRITAEVFRRNGVPEGVSTLVCGGREIGEQ
                     LANDPRVPLVSATGSTRMGKAVGAALGARLGRALLELGGNNAIIISDKADLDIALRAS
                     LFGAVGTAGQRCTTTRRLIIHESVYDLMKQKLVKAYGQLRIGDPLDEKNHVGPLIDTD
                     AVVAYQKALVAAKEQGGSLAVEGGSMTGPGYESGCYVRPAVVEARPEMAITQEETFAP
                     ILYLLKYTGGVENAIAIQNGVKQGLSSSIMTNDMRESERFLSAVGSDCGIANVNIGTS
                     GAEIGGAFGGEKETGGGRESGSDAWKNYMRRQTNTINYGSSLPLAQGIKFDL"
     gene            1027..2304
                     /locus_tag="PL106_07610"
     CDS             1027..2304
                     /locus_tag="PL106_07610"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA32975.1"
                     /translation="MRRTSFFALPVVVLMACTNQPVHQAAWTLREGPWHIELDLRTDT
                     DDQAVRLPFVFDLHSDSGAWSMVIHNGAEAIRVDDVTASPDSFYARPPLFDPYLAGRP
                     LSDSVITGYWYNPLRGAEYRVPFIARAGVSPRFSGSAVPGPDPSGDWACRFMPGSPDE
                     YAALGIFERKDGIVTGTFATETGDYRFLEGVSTGDSLFLSAFDGSHAYLFAASYHGDS
                     LVGQFRSGTHSNERWVASRHAGFQLRNPDSLTFLKPGHSVLDLLLPDLDGHPVSPGHE
                     RFFGHVRIVQLMGSWCANCMDETALLNDLYGRYHPKGLDVIAVGFEKHPDPTKAREAL
                     QRYKERLGLRYDVLYGGQAGQQTTAAQLPFLERLVSYPTLIIIDRAGNVRRINTGFYG
                     PGTGKHHALFKRELTAFLEGLLAESGPTTVATR"
BASE COUNT          448 a          734 c          710 g          432 t
ORIGIN      
        1 tttggaaacc cagtgaaaaa accccgatct gcggtgtcgt gtgccagcgg atcacggccg
       61 aggtcttccg ccgcaacggt gtgcccgaag gcgtgagcac gctggtatgc ggtggtcgcg
      121 aaatcggtga acagctcgcg aatgacccac gtgttccgct tgtgagcgcc accggaagta
      181 cccgtatggg caaggccgtg ggtgcagctc tgggtgcccg tctcggcagg gctctcctgg
      241 aactgggagg caacaacgcg atcatcattt ctgacaaggc cgacctcgat atcgccctgc
      301 gcgcttcgct cttcggcgca gtgggaaccg ccggccagcg ctgcaccact acacgacgcc
      361 tcatcatcca cgagtcggtc tacgacctga tgaaacagaa gctggtgaag gcatacggcc
      421 aactgcggat cggtgatccc ctggacgaga agaaccacgt ggggcccctg atcgacacgg
      481 atgccgttgt ggcgtaccag aaggcgctcg tagcggccaa ggagcagggg gggagcctcg
      541 ccgttgaggg cggctccatg accggacccg ggtacgagag cggatgctat gtgagaccgg
      601 ccgtggtgga ggccaggccc gaaatggcga tcacccagga agagaccttc gcacctatcc
      661 tgtacctgct gaaatatacc ggcggcgtgg aaaacgccat cgccatccag aacggcgtga
      721 aacagggtct ctcctccagc atcatgacca atgacatgcg ggaaagcgaa cgcttcctga
      781 gcgccgtcgg cagcgattgc ggtatcgcca acgtgaacat cggcaccagc ggggcggaga
      841 tcggcggggc cttcggtggc gaaaaggaaa ccggtggtgg ccgtgagagt ggcagcgacg
      901 catggaagaa ctacatgcgc cgccagacca acacgatcaa ctacggctcg tcgttgccac
      961 tggcccaagg gatcaaattc gacctctgac ctgaagcgca ccacacggga acgaaccggg
     1021 gccaccatgc gccgcacctc tttcttcgct cttccggtcg tcgtactcat ggcctgcacc
     1081 aaccaaccgg tccaccaggc cgcatggacg ttgcgtgaag ggccctggca cattgaactg
     1141 gacctgcgca cggacacgga cgatcaagcc gtgcgccttc ccttcgtgtt cgatctgcat
     1201 tctgacagcg gtgcctggtc catggtcatt cataacgggg cagaggccat ccgggtggat
     1261 gatgtgaccg cctctcccga cagtttctac gcgcggccac ctttgttcga cccctacctc
     1321 gccgggaggc cgttgtcgga cagcgtgatc accggttact ggtacaatcc acttcgcggc
     1381 gcggaatatc gcgtcccgtt catcgcccgg gcgggggtat caccacgctt ctccggatca
     1441 gccgtgccgg gaccggatcc ctcgggcgac tgggcttgtc gtttcatgcc cggttcaccg
     1501 gacgaatatg ccgccttggg gatcttcgaa cggaaggacg gcatcgtgac cggcaccttc
     1561 gccacggaaa ccggcgacta tcgcttcctg gagggtgtat ccaccgggga ttcgctgttc
     1621 ctctccgcct tcgacggctc gcatgcctac ctcttcgccg catcctatca tggcgattcg
     1681 cttgttggtc agttccgcag cggcacccat tccaatgagc gctgggtagc ctcccgtcat
     1741 gcgggtttcc aactccgcaa ccccgattcg ctcactttcc tgaaaccggg tcacagtgtg
     1801 ctcgacttgt tgcttccgga cttggacggg catccggtct caccgggcca tgaacgcttt
     1861 tttggccatg tacgcatcgt acagttgatg ggcagctggt gtgccaactg catggatgag
     1921 accgcgctgc tgaatgacct gtacggacgc tatcacccca agggactgga tgtgatcgcc
     1981 gttggattcg agaaacaccc ggatcccaca aaggcccgcg aggccttgca gcgatacaag
     2041 gagcgcctgg gtttgcggta cgatgtgctg tacggcggcc aggcgggcca gcaaacgacc
     2101 gcagcacagc tgcccttcct ggaacgtctg gtgagctacc ccacgctcat catcatcgac
     2161 cgtgccggca acgtacgccg gatcaatacc ggcttctatg gtcccggtac cggcaagcac
     2221 catgcgttgt tcaagcgcga actgaccgcc ttcctggagg gcctgctggc cgaatccggt
     2281 ccaacgacgg tcgccacccg ttagagcggg catgcggggg catg
//
LOCUS       DAOQOC010000427         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28261_length_2321_cov_3.493380, whole genome shotgun sequence.
ACCESSION   DAOQOC010000427 DAOQOC010000000
VERSION     DAOQOC010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28261_length_2321_cov_3.493380"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PL106_07615"
     CDS             <1..370
                     /locus_tag="PL106_07615"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HNA32976.1"
                     /translation="SNTSDAQVQASALNAAIKAVDMAKLDHAVHAVWMQVMEPLANVS
                     SSMAANKGLEKQRKAFVELTAPLAQLAKVAPQPTPIYLDHCPMYIGGADWLSREKAIK
                     NPYYGSSMLTCGSVKETIGK"
     gene            482..>2321
                     /locus_tag="PL106_07620"
     CDS             482..>2321
                     /locus_tag="PL106_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA32977.1"
                     /translation="MGTSRITEPFTPKVTRYDLVITDTIVNYTGKARKAYAVNGSIPM
                     PTLTFTEGDTALIVVHNAMKKEETSLHWHGLILPYQLDGVPYLTTAPIKAGETQVYKF
                     PLVQSGTYWYHSHTNVQEQNGMHGAFIIQKRNAPPMPEHVVILSEWSDMKPIEVHRRL
                     HTANDWSALKKHRIRPGTVQSYSDAIKAGALGVKLTNEWKRMNAMDVSDVYYDRLLAN
                     GRTEDEAPQLKAGDRVRIRLINGGASSYFWISYAGGKMTVVASDGADVEPVEVDRFIM
                     GVAETYDIIVTLPADSTAYELLATSEDRVRSTSVWLGSGIRQLASPLQPLKYFEGMRM
                     MNGMMKMNGDLDDMGMHMSLQQMDMNVVMYPEITGAQKRKGEKGEKGEKGHAAHGVHG
                     GDQYNSNALSEITTLNYAMLRSPVSTALPPGPVKELKFTLTGNMDRYLWAIDNKTVSE
                     TDRILIHKGENVRLILYNNSMMRHPMHLHGHFFRVVNGQGDHAPLKNVLDIMPMETDT
                     IEFAATEEGDWFFHCHILYHMMSGMGRVFTNPPAEPNPALPDARKAWRHFLREDKAWH
                     LMATNDFATNGLDGEAMLMNKRWALQTEWRLGYNAMHGQEVEAHFGR"
BASE COUNT          525 a          733 c          712 g          351 t
ORIGIN      
        1 ttccaacaca tcggatgcac aggtccaggc gagcgcgttg aatgctgcga tcaaggcggt
       61 ggacatggcg aagctcgacc acgccgtcca tgccgtgtgg atgcaggtga tggagccgct
      121 ggcgaacgtg agcagcagca tggccgccaa caagggcctg gagaaacagc gcaaggcctt
      181 cgtggagctg accgcacccc tggcacaact cgccaaagtc gcgccgcagc caacgcccat
      241 ctacctggac cattgcccca tgtacatcgg cggcgccgac tggctcagcc gcgaaaaggc
      301 gatcaagaac ccgtactacg ggagcagtat gctcacttgc ggcagcgtga aggagaccat
      361 cggcaagtaa gcgaccagtc aactgccgaa catgtccgtg atacgttcca tcggatcctg
      421 cctggtggcc ctgctgttgc ttgctgctgg atcgcacgca cagtataccc cgcatccggg
      481 cctgggcacc tcgcgcatca cggagccctt caccccgaag gtgacgcgtt acgacctggt
      541 gatcaccgac accatcgtca actacaccgg caaggcgcgc aaggcctacg cggtgaacgg
      601 gagtattccc atgcccacgc tcaccttcac cgagggcgat accgcgctga tcgtggtgca
      661 caacgcgatg aagaaggaag agaccagcct gcactggcac ggcctgatcc ttccgtacca
      721 actggacggc gtgccctacc tcaccaccgc gcccatcaag gccggggaga cgcaggtgta
      781 caagttcccg ctggtgcaga gcggcaccta ctggtaccac tcgcacacca acgtgcagga
      841 gcagaacggc atgcacggcg ccttcatcat ccaaaagcga aacgcgccgc ccatgcccga
      901 gcacgtggtg atcctgagcg agtggtccga catgaagccc atcgaggtgc atcgccgcct
      961 gcacacggcg aacgactgga gcgcactgaa gaagcaccgc atccggccgg gcacggtgca
     1021 gagctacagc gatgcgatca aggccggagc actgggcgtg aagctcacca acgagtggaa
     1081 gcgcatgaac gccatggacg tgagcgacgt gtactacgac cgtctgctgg ccaatggccg
     1141 tacagaggat gaagcaccac agctgaaggc gggcgaccgg gtgcgcatcc gcctgatcaa
     1201 cggcggcgca agcagctact tctggatcag ctatgccggt ggcaagatga ccgtggtggc
     1261 cagcgacggc gccgacgtgg agccggtgga agtggaccgc ttcatcatgg gcgtggccga
     1321 gacctacgac atcatcgtta cgcttcccgc cgacagcacg gcatacgaac tgttggccac
     1381 cagcgaggac cgcgtgcgct ccacctcggt gtggctcggc tcgggcatcc gccagctcgc
     1441 atcgcccctg caaccgttga agtacttcga gggcatgcgc atgatgaacg gcatgatgaa
     1501 gatgaacggc gacctcgacg acatgggcat gcacatgagc ctgcagcaga tggacatgaa
     1561 cgtggtgatg tatccggaga tcacgggagc gcaaaagagg aagggtgaga aaggtgaaaa
     1621 gggtgagaaa gggcatgcag cgcacggcgt gcacggcggc gatcagtaca acagcaacgc
     1681 gctatcggag atcaccacgc tgaactacgc catgctgcgc tcgccggtga gtaccgctct
     1741 tccgccgggg ccggtgaagg agctgaagtt cacgctcacc ggcaacatgg accgctacct
     1801 ctgggccatc gacaacaaga ccgtttcgga gaccgatcgc atcctgatcc acaagggcga
     1861 gaacgtacgc ctgatcctgt acaacaacag catgatgcgc caccccatgc acctgcacgg
     1921 ccacttcttc cgcgtggtga acggccaggg cgatcatgct ccgctgaaga acgtgctgga
     1981 catcatgccg atggaaacgg acaccatcga gttcgctgcc accgaggaag gcgactggtt
     2041 cttccactgc cacatcctct accacatgat gagcggcatg ggccgcgtgt tcaccaatcc
     2101 gcctgcggag cccaaccccg cgctgcccga tgcgcgcaaa gcgtggcggc acttcctgcg
     2161 cgaggacaag gcctggcacc tgatggccac gaacgacttc gccaccaacg ggctcgatgg
     2221 cgaggccatg ctaatgaaca aacgctgggc cttgcaaact gaatggcgct tgggctacaa
     2281 cgcgatgcac ggccaggagg tggaagcgca tttcggacgc t
//
LOCUS       DAOQOC010000428         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28286_length_2320_cov_3.343488, whole genome shotgun sequence.
ACCESSION   DAOQOC010000428 DAOQOC010000000
VERSION     DAOQOC010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28286_length_2320_cov_3.343488"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PL106_07625"
     CDS             <1..256
                     /locus_tag="PL106_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32978.1"
                     /translation="DEVAAGKLYNDKVVRVSGVVREVGTESTGPVNVLLETGDNLGAV
                     VCEFERATAPALQKGQQVSLQGFCAGFNLDVLLQRCATVE"
     gene            295..837
                     /locus_tag="PL106_07630"
     CDS             295..837
                     /locus_tag="PL106_07630"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNA32979.1"
                     /translation="MIRPFALSLLLIPATTFAQERYATRNGEVSFFSSTPMEDITAMN
                     HKATCVMDLTSGRIQVSMLIKAFEFEKALMQEHFNENYMESNTFPKADLQGTLKGLSA
                     EDTKKPGKYAVTIDGELTIHGVVQKRSIPGTIEVDAAGAMKATAEFIVKPADHGIRIP
                     GDVNVAKEIRVNAALDLRKM"
     gene            869..1801
                     /locus_tag="PL106_07635"
     CDS             869..1801
                     /locus_tag="PL106_07635"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNA32980.1"
                     /translation="MRKLLPVLFALLFLPTRAQDDLLSLLGQDSAVKEYTNASFKTTR
                     VINAHSLENTAHGVLDARINHRFGLLSEGVGNFFGLDESTIRIGFDYGVTDRLMIGIG
                     RSSYQKTIDGFAKYKILRQCDAGCTMPVTLAVVASTAVTTLQADEVPWYEEGRKDYFS
                     HRLSYSFQVILGRKFSDAFSMQIMPGSVHRNLVATVDDRNDIFNVGVAARYKFLRRLA
                     INTEYFYVLPDQGPRDAYNNSLSVGFDIETGGHVFQLHFTNSTGMFERAFITETSGDF
                     FKGRIHYGFNISRVFTLYDPKKKLRERKRKEGQQ"
     gene            complement(1909..2106)
                     /locus_tag="PL106_07640"
     CDS             complement(1909..2106)
                     /locus_tag="PL106_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32981.1"
                     /translation="MNTKKLIGMACWLLAFLLPARYSLVESEQASNLGGLVWFLAFLF
                     LMFLGFWLVESSAAKRADHGH"
     gene            complement(2159..>2320)
                     /locus_tag="PL106_07645"
     CDS             complement(2159..>2320)
                     /locus_tag="PL106_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNA32982.1"
                     /translation="PTRMFTVMFALGRLPGWIAQWKEMVDNKEPIGRPRQIYTGATER
                     AYVPLKNRA"
BASE COUNT          487 a          718 c          688 g          427 t
ORIGIN      
        1 cgacgaagtg gctgcgggca aactgtacaa cgacaaagtg gtgcgggtct ccggcgttgt
       61 ccgggaggtc ggaaccgaaa gcacggggcc ggtgaatgtg ctgctggaga ccggcgacaa
      121 cctgggtgcc gtggtctgcg aattcgagcg tgccacggca cccgcattgc aaaagggcca
      181 gcaggtctcc ctgcagggct tctgtgcggg cttcaacctt gacgtgctgt tgcagcgctg
      241 cgccactgtg gaatgatact tggaacccga ctgacagaac gaaccgataa ccccatgatc
      301 cgcccattcg ccctgagcct tctgctcatc cccgccacca ccttcgcaca ggaacggtat
      361 gccacccgca acggcgaggt ctccttcttc tccagtaccc ccatggagga catcaccgca
      421 atgaaccaca aggccacctg cgtcatggac ctcaccagcg ggaggatcca ggtgagcatg
      481 ctgatcaagg ccttcgagtt cgagaaggcg ttgatgcagg agcacttcaa cgagaactac
      541 atggagagca acacctttcc caaggccgat ctgcagggca cgttgaaggg cttgagcgcc
      601 gaggatacga agaaacccgg caagtacgcg gtgaccatcg atggtgagct caccatccac
      661 ggggtggtac agaagaggag catccccggc accatcgagg tggacgccgc cggtgcgatg
      721 aaggccacgg ccgagttcat cgtgaagccc gccgaccatg ggatcaggat cccgggtgat
      781 gtgaacgtgg ccaaggagat ccgcgtgaac gcggccctgg acctccgaaa aatgtgatcc
      841 gtacaggtat tatcccagcc cgacccgcat gcgcaaactg cttccagtcc tgttcgccct
      901 gctgttcctg cccacccggg ctcaggacga cctgctgagc ctgctcgggc aggacagtgc
      961 ggtgaaggag tacaccaatg ccagcttcaa gaccacacgt gtgatcaatg cgcacagcct
     1021 ggagaacacc gcgcatggtg tgctggacgc ccgcatcaat caccgcttcg gcctgctttc
     1081 cgagggcgtg ggaaacttct tcggattgga cgagtcgacc atccggatcg gcttcgacta
     1141 cggggtgacc gaccggttga tgatcggcat cggccgcagc agctatcaga agaccattga
     1201 cggattcgcg aagtacaaga tcctgcggca atgcgatgcg ggatgcacca tgccggtcac
     1261 cctggccgtg gtggcctcga cagcggtgac caccttgcaa gccgatgagg tgccctggta
     1321 cgaggaaggt cgcaaggact atttctcgca ccgcctttcc tattccttcc aggtcatcct
     1381 tggccgcaag ttcagcgacg cgttctccat gcagatcatg cccggatcgg tacaccgcaa
     1441 cctggtggcc acggtggatg accgcaacga catcttcaat gtcggcgtgg ccgcccgtta
     1501 caagttcctg cgccgcctgg ccatcaatac cgagtatttt tatgtgctgc ccgatcaagg
     1561 cccccgcgat gcctacaaca actcactctc cgtcggtttc gatatcgaaa ccggtggcca
     1621 cgtgttccag ttgcatttca ccaatagcac gggcatgttc gagcgcgcgt tcatcaccga
     1681 aacgagcggc gacttcttca aggggcggat ccactacggc ttcaacatct ctcgcgtgtt
     1741 caccctgtac gacccgaaga agaagctccg cgagcggaag aggaaggagg ggcaacagtg
     1801 atcgtgcctt gaatggggcg cgccgtgaac agggctcgca gcgggttctg tgcgctccgt
     1861 tgtggaacgc ggacagggct tagcccatcc cgtggccgga acgatacctc agtgcccgtg
     1921 gtccgcacgc ttggcggccg aactctccac cagccagaag ccgaggaaca tcaagaacag
     1981 gaaggccagg aaccagacca atccgccaag gttgcttgcc tgttcgcttt ccaccagcga
     2041 atagcgggcg ggcaaaagga aggcgaggag ccaacaggcc ataccgatca gtttcttggt
     2101 gttcatccct tgtgtgcttg gttcggcgaa ttaacgtctt ccccctgaat gtcgcacctc
     2161 acgcacggtt ctttaacggc acgtacgcac gctccgtggc gccggtgtag atctggcgcg
     2221 ggcgcccgat gggctccttg ttgtccacca tctccttcca ctgcgcgatc cagcccggca
     2281 gccgacccag ggcgaacatg acggtgaaca tgcgcgtggg
//
LOCUS       DAOQOC010000429         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28311_length_2319_cov_3.924028, whole genome shotgun sequence.
ACCESSION   DAOQOC010000429 DAOQOC010000000
VERSION     DAOQOC010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28311_length_2319_cov_3.924028"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PL106_07650"
     CDS             complement(<1..333)
                     /locus_tag="PL106_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA32983.1"
                     /translation="MKTRQLGRTGPISSAIGLGCMGMSDLYGAGDDAESIATIHAALD
                     AGITLLDTGDYYAMGHNELLIREALKGRDRSKALISVKFGALRDPGGNWLGFDGRPAA
                     VKNALAYTL"
     gene            complement(407..796)
                     /locus_tag="PL106_07655"
     CDS             complement(407..796)
                     /locus_tag="PL106_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA32984.1"
                     /translation="MPVRKIRRPSRPQELSAAYMKALDLHMAEFRAGTADRAWGIRDF
                     AERLFVHPTHLSNTLQEVTGRSPCDIYEERLMDVARELLIGSKGSAAEVARQLTMDPS
                     NFNKFFKRFEGITPKQFRELRAMRHSA"
     gene            complement(841..1539)
                     /locus_tag="PL106_07660"
     CDS             complement(841..1539)
                     /locus_tag="PL106_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA32985.1"
                     /translation="MKIGVFGTGVVGQVLADKLDSLGHTVVLGTRDVKQSLGREGQDG
                     YGRPALKEWLAAHPKVQLGTFAEAAAHGSLLVNATSGTGALQALKLAGEGHLNGKVML
                     DISNPLDFSKGMPPSLSVCNTDSLGEQIQRAFPQLKVVKGLNTLTSFLMVAPRMLPDT
                     HHIFLCGNDAGAKGEVRSLLSSFGWAGEEMIDLGDITNARGTEQLLPIWVRLYGAMQN
                     PMFNFKVVVVPKQA"
     gene            1692..2105
                     /locus_tag="PL106_07665"
     CDS             1692..2105
                     /locus_tag="PL106_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA32986.1"
                     /translation="MVIDGDEYRIRLNGHVYHCALDVTMELVGGKWKTIVLWYLRKDK
                     KRFSELRKLIPGITEKMLSLQLRQLEKDGFVSRKVYPEVPPRVEYALTAHGRTLLPLL
                     EEIAAWGRMMGKKYGSVEKVDRPAKKKAARKNVST"
BASE COUNT          440 a          724 c          715 g          440 t
ORIGIN      
        1 caacgtatag gccagtgcgt tcttcaccgc cgccgggcgc ccatcgaagc cgagccagtt
       61 gccgccggga tcgcgcagtg cgccgaactt cacgctgatc agggccttcg accggtcacg
      121 gcctttcagc gcttcgcgga taagcagttc gttgtggccc atggcgtagt agtcgccggt
      181 atcgagcagg gtgatgcccg catcgagcgc ggcgtggatg gtggcgatgc tctcggcatc
      241 atcgccggcg ccgtagaggt cgctcatgcc catgcatcca aggccgatag cggagctgat
      301 ggggccggtc ctgcccagtt gacgggtctt catggtcgta gagatgaggg cacaaagctg
      361 aagcgcacgc cgacggccgg catggtgaga acttcagaat gtgtgctcag gcactgtggc
      421 gcatcgcccg cagttcgcgg aactgcttcg gtgtgatccc ctcgaaccgt ttgaagaact
      481 tgttgaagtt gctcggatcc atggtgagct ggcgggccac ttcagcagcc gaccccttcg
      541 agccgatcag cagttcgcgc gccacgtcca tcaaccgttc ctcgtagatg tcgcatggcg
      601 aacgtcccgt gacctcttgc agcgtgttgc tcaagtgcgt cgggtgaacg aagagccgtt
      661 cagcgaaatc ccggatgccc cacgctcgat cggccgtacc ggcgcggaac tccgccatgt
      721 gcaggtccag cgctttcatg taggcggcgc tcagctcttg tggccgggac ggcctgcgga
      781 tcttgcggac gggcattcga tgcaaaggtg tgctcaaacg ccgttcacca gtgcgctcct
      841 tcacgcctgc tttggcacga ccaccacctt gaaattgaac atggggttct gcatggcccc
      901 gtacaggcgc acccagatgg gcaacagctg ctcggtgccg cgtgcgttgg tgatgtcgcc
      961 caggtcgatc atctcctccc cggcccagcc gaaggacgag agcagcgatc gcacttcgcc
     1021 cttggcaccg gcatcattgc cgcataggaa gatgtggtgc gtatccggca gcatgcgcgg
     1081 cgccaccatc aggaagctgg tgagcgtgtt gaggcccttc accaccttca gttgcggaaa
     1141 ggcgcgctgg atctgttccc cgagcgaatc ggtgttgcat accgaaaggc tgggcggcat
     1201 gccctttgaa aagtccagcg ggttggaaat gtcgagcatc accttgccgt tgaggtggcc
     1261 ttcgcctgcg agcttcaacg cttgcagggc gccggttccg ctggtggcgt tcaccagcaa
     1321 gctgccgtgg gccgcagcct ccgcgaaggt gcccagttgc actttgggat gcgcggccag
     1381 ccactccttc agggccgggc ggccgtagcc gtcctggcct tcccgcccga ggctctgctt
     1441 cacgtcgcgc gtgccgagca ccaccgtatg gcccaggctg tcgagcttat cggcgaggac
     1501 ctgccccacc acacccgttc cgaatacacc gatcttcatg gtcgttcgtg ttggtttctt
     1561 tttctttgtc gctgcaaagg tgtccggcct ctatactttc cacaagtact acccatcttg
     1621 acagtatcga acatggatgc cgggttcagc ggcatggacg ggcgggaaca ccagcacaag
     1681 ttttcaacac catggtcatc gacggcgacg aataccgcat ccgcttgaac ggccacgtgt
     1741 accactgcgc cctggacgtg accatggagc tggtgggcgg caagtggaaa accatcgtgc
     1801 tgtggtacct gcgcaaggac aagaagcgct tcagcgagct gcgcaagttg atccccggca
     1861 tcaccgagaa gatgctttcc ctgcaactgc ggcaactgga gaaggacggc ttcgtgagcc
     1921 gcaaggtcta tccggaagtg ccaccacgcg tggagtatgc gctcaccgcc cacggcagaa
     1981 cgctcctgcc cttgttggag gagatcgccg cgtggggccg catgatgggc aagaaatacg
     2041 gcagcgtgga aaaggtggac cggccagcga agaagaaggc ggcacggaag aacgtaagca
     2101 cttagcctac cgtccgaact accttggcag gaccgaacga acacccatgt cccgacacgt
     2161 caccggcatc ggcggattct tcttccgcgc caacgatcac aaggccctcg ccaagtggta
     2221 caacgaccac ttcggcatca acgaccagga caacggctgg atctggcagc aggatgccgg
     2281 gcccacggtg ttctcgccct tcaagcgcga cagcgacta
//
LOCUS       DAOQOC010000430         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28323_length_2319_cov_2.893551, whole genome shotgun sequence.
ACCESSION   DAOQOC010000430 DAOQOC010000000
VERSION     DAOQOC010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28323_length_2319_cov_2.893551"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PL106_07670"
     CDS             complement(<1..897)
                     /locus_tag="PL106_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32987.1"
                     /translation="MGRPDRVVFLAFAGVVGLYVAMRAALVPMVHDECATVAWYIRSG
                     VWRPYQAHWDAGNHYLATGIGLLSARLFGGSPFALRAGDLLAYVLYAYATWRLGARFR
                     SRTPRLCLQAALLLCPYLLDFFSLFRGYGIEMAGWLFALDGLLRYVLSKASRHLFQTL
                     VGLLVAGASIVALVPVWALVLVLLAAVGWGARDTRWTQLTLWTLFGVVPLILASAIAF
                     QLKARGLLYHGSTDGFFAVSVTSLCRYVLGSTAVLTASIVCLVLLALGMVAVQHAWRA
                     STWRDPMVLIYLLLGCDVLARVM"
     gene            complement(904..1602)
                     /locus_tag="PL106_07675"
                     /pseudo
     CDS             complement(904..1602)
                     /locus_tag="PL106_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202766.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
     gene            complement(1679..>2319)
                     /locus_tag="PL106_07680"
     CDS             complement(1679..>2319)
                     /locus_tag="PL106_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA32988.1"
                     /translation="NHVFLPSMTDSLVNFQFTTTDTIVAWFKPPTQYPIVLMTDPPNT
                     ATVEFNGQTYTSFPVVVNVGEDVPVDVSATPNTFYDFVYWEVRHNIPNTNDSTRPDFT
                     INFFGPDTLIAHLKPQEYGFWAPNAFTPNGDGINDTWLPWGNVIDPEGFDLQVYDRWG
                     QLMYASNNPRDGWDGTVGGTPAPLGVYAYRGHIIEGVTHAKHDLKGHITVIR"
BASE COUNT          434 a          744 c          736 g          405 t
ORIGIN      
        1 catcacccgc gccagcacat cgcacccgag caggaggtat atcaggacca taggatcacg
       61 ccacgtgctg gctcgccacg catgctgcac ggcgaccatg cccaaggcca ggagtaccaa
      121 gcatacgatg gatgccgtga gcacggcggt gctgcccagc acgtatcggc acaggctcgt
      181 tacggagacc gcgaagaacc cgtcggtgct tccgtggtac aataagccac gggccttgag
      241 ttggaacgcg atggcgcttg ccaagatcaa tggcaccacc ccgaacagcg tccagagggt
      301 cagctgtgtc caccgcgtat cacgcgctcc ccaccccacc gcggccagca gcacgagcac
      361 caaggcccac acgggtacca gcgccacgat ggaagcaccc gccaccaaca gcccgacaag
      421 cgtctgaaaa agatggcgcg aggccttcga caggacatag cggaggaggc cgtcgagcgc
      481 gaacaaccaa ccggccattt cgataccgta tccccggaac agtgagaaga aatcgagcag
      541 gtagggacag agcaacaatg cggcctgcag gcacaagcgt ggggtccgcg aacggaaccg
      601 ggcccccagg cgccatgtcg catacgcata caggacgtac gcgagcagat cccctgcgcg
      661 cagggcgaac ggggatccgc cgaagaggcg tgcggacaac aggcctatac cggtagccag
      721 gtaatggttt ccagcgtccc agtgcgcttg gtacggtcgc cacaccccgg accggatgta
      781 ccaggccacc gtggcgcact cgtcgtggac catgggcacc agggcggcgc gcatggcgac
      841 ataaagcccg accacacccg cgaacgcaag gaacacgaca cggtccggcc ggcccatggc
      901 catttacgcc cgttgccgca cggtgcgctg ctcgatccgc ttctcgtagt tgctgccctg
      961 gaagatgcga tgcacgaaga tgcccggcgt gtgtacctcg tccgggtcga tctcaccggg
     1021 ctgcaccagg tgctccacct cggccacggt gatccggccg gccatggcca tcatggggtt
     1081 gaagttgcgc gccgtacggc ggtacaccag gttgccgttg gtgtcgccct tccaggcctt
     1141 gatcaggctg aagtccgcgc ggagtcaatg ctccatcacg tacatcttgc catcgaactc
     1201 gcgggtctcc ttgccttcgg ccacttcggt accgtagccg gccggggtga agaaggccgg
     1261 gatgcccgcg ccgcccgcgc gccagcgttc ggccagcgtg ccttgcgggg tgagttgcac
     1321 ttccagttcg cccgagagca gttgccgttc gaattcggcg ttctcaccca cgtagctggc
     1381 gatcatcgtc ttgatctgcc gctgtttcaa taacaggccc aggccgaagt cgtccactcc
     1441 ggcgttgttg ctgatgcagg tgaggccctt cacgccgttg cgcaccaggg cgccgatcag
     1501 gttctccgga atgccgcaca atccgaagcc tcccaccatc aatgtcatgc cgtccttgat
     1561 gcctgcgagc gcggcatccg cgttggccac cgtcttttcc atgcgttgaa cgccgcaccc
     1621 gcgttgagcg gcgaggcggt gggccgaatg tacacggcct gcgcgaagcg cgccgcgctc
     1681 agcggatcac cgtgatgtgc cccttcaggt cgtgcttcgc gtgggtgacc ccttcgatga
     1741 tgtgcccgcg atacgcgtac acgccgagcg gtgcgggcgt gccgcccacg gtgccgtccc
     1801 atccgtcacg cgggttgttg cttgcgtaca tcaactgtcc ccagcggtca tatacctgga
     1861 ggtcgaaacc ttccggatcg atcacgttgc cccagggcag ccaggtgtcg ttgatgccat
     1921 ccccgttcgg ggtgaacgca ttgggcgccc agaagccgta ctcctgtggc ttcaggtgcg
     1981 cgatcaaggt gtccggtccg aagaagttga tcgtgaagtc cggacgggtg gagtcgttcg
     2041 tgttcgggat gttgtggcgc acttcccagt acacgaagtc gtagaaggtg ttcggtgtcg
     2101 cgctcacatc cacgggcaca tcctcgccca cgttgaccac gaccgggaag ctggtgtacg
     2161 tctgcccgtt gaactcaacg gtggccgtgt tgggcgggtc ggtcatgagg acgatgggat
     2221 attgggtggg gggcttgaac caggccacga tggtgtccgt ggtcgtgaac tggaagttca
     2281 cgagcgaatc ggtcatgctg ggcaggaaca cgtggttgt
//
LOCUS       DAOQOC010000431         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28346_length_2318_cov_2.778612, whole genome shotgun sequence.
ACCESSION   DAOQOC010000431 DAOQOC010000000
VERSION     DAOQOC010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28346_length_2318_cov_2.778612"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..416)
                     /locus_tag="PL106_07685"
                     /pseudo
     CDS             complement(237..416)
                     /locus_tag="PL106_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            543..695
                     /locus_tag="PL106_07690"
     CDS             543..695
                     /locus_tag="PL106_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32989.1"
                     /translation="MAWILSKRTAYVEIVSSGHDLHVWRSPGEHRYTIGPNAECVPFA
                     QLEKLM"
     gene            1042..1218
                     /locus_tag="PL106_07695"
     CDS             1042..1218
                     /locus_tag="PL106_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32990.1"
                     /translation="MHDFYFYNVHSGSELDMFFLYKGKRVGVEIKRQDAPRMTKSIPL
                     PSTHDRSRPVYGSR"
     gene            1275..>2318
                     /locus_tag="PL106_07700"
     CDS             1275..>2318
                     /locus_tag="PL106_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32991.1"
                     /translation="MKRTHYAVGLLCSMIGQGAAAQAPTLVKDFWTGSFGAYMNHGNP
                     GAMRLHGDDLLVFATDGPTAAKLFRLNDLSGNVEELADVGYAAGYTTTNGGIITSGDN
                     IYFFSQQSGSTNFLLWCVRDGADAEQLAVLPYSLLFGFVGIPLPDGKLLFPGHDADHG
                     FELWVTDGTVAGTDRVKDINPGTGTSFGSPFPAFQGFDFEGKAYFLANDGTNGPQLWV
                     SDGTEAGTTQFATINEANTSGAVTMLWSRNDDSFIVTGHTGLIASDGTTAGTSVIHPG
                     EYAWAHIPGFNYHTADNGFMYFSALENSDWKLYRTQGTTATTELVVEDVVPNQGYPYM
                     TELNGMLYAFWIND"
BASE COUNT          482 a          673 c          702 g          461 t
ORIGIN      
        1 aatgccgcag cccttcgctg atggcccgtt cctcatggcg cttcaacaag atcagcacaa
       61 agtcgagcac ctcctccgct ttttcctgcg gaagtttatt cagcgcacca atggtgcgtt
      121 cgatcaaggc ttgtttggtc atgcacgttc gttgcttctt caaaagtaga cctttgatgg
      181 gcaatgcagg aaatgcgcca ccgggtactc ccacccgtac cattgcgtct attagcgaag
      241 aagtgcaggg cgttgcgcag ggcgagcttt ttctcttcgg cggaaaggat gtccttgcgc
      301 ttgggggcgt ggcttacgcc gggatccagc gggcgggctg gaggaaggat atcgggaatg
      361 tcttcgcgga tggcggcttg gaaggcggtg aggtcggaag tggttgcgga aggcattggg
      421 cgacgatcgg tcagggagcg cattccgcgc acgtggggca cggggaagag cggaagcgct
      481 ggtcttcttc gcggtgggca ccctaacgtg aagatcagaa gcggaatttc tgtttttcat
      541 gtatggcatg gatcctttcc aagcggacgg catacgtgga gatcgtgagt tcaggtcacg
      601 atcttcacgt atggagaagt cccggcgagc accgctacac catcggaccc aacgccgagt
      661 gcgtgccctt cgcgcaactg gaaaagctga tgtgagggag cctcatgaga aagctcaccg
      721 ggtgccttcg cggatggacc gttgagagtg ggcgaggcgc gtggcggggt cggtgggcat
      781 gcgtttgaac gctacgaaaa gggggcggtg tcggcggtgc gaagttcgcg attggatgga
      841 ggaatgggga cctgcttgca cgagatgggt ggttgagtgc agccatgaag tggtttgatc
      901 cttccgcagg gtcttcgcct cgagaccctg cgggatgtga caatcttcgg agatcaaccc
      961 aaaactgtaa acctgtatca ggacgagtca agactttggc tcgcagggtc ccgaagacgg
     1021 agaccctgcg gaaggagaaa attgcacgac ttctacttct acaacgtcca ctccggctcc
     1081 gaactggaca tgttcttcct gtacaaaggc aagcgcgtgg gcgtggagat caagcgccag
     1141 gacgccccgc gcatgaccaa gagcattccc cttccatcaa cgcatgatcg ttcacggccc
     1201 gtttacggat cacggtgaat ccccgtagca actccgccag acgtttgccg ttgaaagccc
     1261 tcaaccacga tacgatgaaa aggacccact acgccgttgg cctgctgtgc agcatgatcg
     1321 gacaaggggc agccgcgcag gcgcccaccc tggtgaagga tttctggacc ggctccttcg
     1381 gggcctacat gaaccacggg aaccccggcg ccatgcgcct ccacggggat gacctgctgg
     1441 tcttcgcgac cgatggaccc actgccgcga agctcttccg tctgaacgac ctcagcggga
     1501 acgtggagga actggccgac gtgggttacg ccgccggtta taccaccacc aatggaggca
     1561 tcatcaccag cggcgacaat atctacttct tcagccagca atccggctcc accaacttcc
     1621 tcctctggtg cgtgcgcgat ggtgccgatg ccgaacaatt ggcggtgctg ccctattcgc
     1681 ttctcttcgg tttcgtgggc attccccttc cggatggcaa gctgctgttc cccggacatg
     1741 atgccgacca tggattcgaa ctttgggtga cggacgggac cgtcgccgga acagaccgcg
     1801 tgaaggacat caaccccggc acgggaacct cgttcggttc gcccttcccc gccttccaag
     1861 gtttcgactt cgagggcaag gcctatttcc tggccaatga cggaaccaat ggccctcaac
     1921 tgtgggtgag cgatggcacg gaggccggta ccacacagtt cgcaacgatc aatgaggcga
     1981 acacgagcgg agcggtcacc atgctctggt ccaggaacga cgactccttc atcgttaccg
     2041 gccacaccgg cctcatcgcc tcggacggaa ccacggccgg cacttccgtg atccatcccg
     2101 gggaatatgc atgggcccac atccccggct tcaactacca tacggccgac aacggcttca
     2161 tgtacttcag cgcgctggag aacagcgact ggaaactcta ccgcacccaa ggcaccacgg
     2221 ccaccacgga gcttgtggtg gaggatgtgg tgcccaatca gggctatccg tacatgaccg
     2281 aactcaacgg tatgctctac gcattctgga tcaatgac
//
LOCUS       DAOQOC010000432         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28376_length_2317_cov_2.731211, whole genome shotgun sequence.
ACCESSION   DAOQOC010000432 DAOQOC010000000
VERSION     DAOQOC010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28376_length_2317_cov_2.731211"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2211
                     /locus_tag="PL106_07705"
     CDS             <1..2211
                     /locus_tag="PL106_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA32992.1"
                     /translation="KNASYTPAWQEIFTGIGRNTAIKFAREWAGTAEATKGRCMVIVG
                     AAINHWFHGNLMYRSAIMAQMLTGCNGRNGGGMNHYVGQEKLAPVDSWGTIMSGKDHG
                     TVPRLQQAPIWHYINSSQWRYDNNQADYNSVPNDANPLARKHSADWVLMSVRNGWMPF
                     FPQYNKSNLDIARDARKEGAQTEADIRDYVVRKLKSKELDYSVADPDDPINFPRNWFI
                     WRGNALMSSAKGHEYMLNHYLGTHHNDIANEVAGEYVTDIKYREKSPSGKMDLVVDIN
                     FRMDTSALYSDIVLPTASWYEKADLNSTDMHSFIHPLSEAVPPVWEAKTDWQIFQSIA
                     KKVSEMAKTHFPAPVTDIVNVALSHDSADEITQPRLLDWTKGECEPIPGKTMHKIVVI
                     DRDYTQIHNKFITLGEGVAKQGLGAHGVKYECADVYQEMLEKKNHVQVVDGKEYPSLR
                     EDVEACDAVLKLSTLTNGKLNMRGYQMLEKKTGLALTDLAEGLQAVRIDYKDLQTRPH
                     RYNTSAVWSGLMNDGRAYAAYTFQVDRLVPWRTLTGRQHIYMDHDGYIMFGEHLPTYK
                     PSPLPQVYGDIRKTVNDGKARMLNCLTPHGKWHIHSTYGDTLRMLTLSRGMEPCWMSE
                     KDAADLGIKDNDWVEVYNDHGVYCTRACVSSRIPRGVCIVYHSPERTYTVPKSQVRGG
                     RRAGGHNSFTRVHLKPSLMVGGYGQFSFHFNYWGPVGANRDTHVLVRKMEKVEF"
BASE COUNT          537 a          756 c          686 g          338 t
ORIGIN      
        1 aagaacgcca gttacacccc ggcctggcag gagatcttca ccggcatcgg ccgcaacacc
       61 gccatcaagt tcgcgcgcga atgggccggc accgccgagg ccaccaaggg tcgctgcatg
      121 gtgatcgtgg gcgcggccat caaccactgg ttccacggca acctgatgta tcgttccgcc
      181 atcatggcgc aaatgctcac cggctgcaac gggcgcaacg gcggtggcat gaaccactac
      241 gtgggccagg agaagctggc accggtggac agctggggca ccatcatgag cggcaaggac
      301 cacggcaccg tgccacgcct gcagcaggcc cccatctggc actacatcaa cagcagccag
      361 tggcgctacg acaacaacca ggcggattac aacagcgtgc cgaacgatgc caacccgctc
      421 gcgcggaaac attccgccga ctgggtattg atgtccgtgc gcaacggctg gatgcccttc
      481 ttcccgcagt acaacaagag caacctggac atcgcacgcg atgcccgcaa ggaaggggcg
      541 cagaccgagg cggacatccg cgactacgtg gtgcgcaagc tcaagagcaa ggaactcgac
      601 tattccgtgg ccgacccgga cgacccgatc aacttcccgc gaaactggtt catctggcgc
      661 ggcaacgccc tgatgagctc ggccaagggc cacgagtaca tgctcaacca ctacctcggc
      721 acgcaccaca acgacatcgc caacgaggtg gccggcgaat acgtcaccga catcaagtac
      781 cgcgagaagt cgcccagcgg caagatggac ctggtggtgg acatcaactt ccgcatggac
      841 acctccgcgc tgtactcgga catcgtgctg cccaccgcat cgtggtacga gaaggccgac
      901 ctgaacagca cggacatgca ctcgttcatc cacccgttga gcgaggctgt accgccggtg
      961 tgggaagcca agactgactg gcagatcttc cagtccatcg ccaagaaggt gagcgagatg
     1021 gcgaagacgc acttcccggc gcccgtcacc gacatcgtga atgtggcact ctcgcacgac
     1081 agcgccgacg agatcaccca gcccaggctc ctggactgga ccaagggcga gtgcgagccc
     1141 atccccggca agaccatgca caagatcgtg gtgatcgacc gcgattacac gcagatccac
     1201 aacaagttca tcaccctggg cgaaggtgtc gccaagcagg gcctgggcgc gcacggtgtg
     1261 aagtacgagt gcgcggatgt gtaccaggag atgctggaga agaagaacca cgtacaggtg
     1321 gtggacggca aggagtaccc ttcgctgcgc gaggacgtgg aagcctgcga tgccgtgctc
     1381 aagctcagca ccctcaccaa cggcaagctg aacatgcgcg gctaccagat gctggagaag
     1441 aagaccgggc ttgcactgac cgaccttgcc gaagggctgc aggccgtacg gatcgactac
     1501 aaggacctgc aaacgcggcc gcatcgctac aacacctccg ccgtatggag cggtctgatg
     1561 aacgacggtc gtgcatacgc cgcttacacc ttccaagtgg accgcctggt tccgtggcgc
     1621 acgctcaccg gccgccagca catctacatg gaccacgacg gctacatcat gttcggcgag
     1681 cacctgccca cctacaagcc ctcgccgctg ccgcaggtgt acggcgacat ccgcaagacg
     1741 gtgaacgacg gcaaggcccg catgctcaac tgcctcacgc cccacggcaa gtggcacatc
     1801 cacagcacct acggcgacac cctgcgcatg ctcaccctga gccggggcat ggaaccctgc
     1861 tggatgagcg agaaggacgc cgctgacctg ggcatcaagg acaacgattg ggtggaggtg
     1921 tacaacgacc acggcgtgta ctgcacccgg gcctgcgtca gctcacggat cccgcgtggc
     1981 gtgtgcatcg tgtaccactc gcccgagcgc acgtacacgg tgcccaagag ccaggtgcgc
     2041 ggcggcagac gtgccggcgg ccacaacagc ttcacccgtg tacacctcaa gcccagcctg
     2101 atggtgggcg gttatgggca gttcagcttc cacttcaact actgggggcc cgttggtgcc
     2161 aaccgcgata cccacgtgct ggtacggaag atggagaagg tggaattctg atacgaacaa
     2221 gaaggaacag gccagcagac ctcaaaccga acagccaaag ccatgaacat cagatcacag
     2281 atctcaatgg tcttccacct cgataagtgc atcgggt
//
LOCUS       DAOQOC010000433         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28393_length_2316_cov_3.130916, whole genome shotgun sequence.
ACCESSION   DAOQOC010000433 DAOQOC010000000
VERSION     DAOQOC010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28393_length_2316_cov_3.130916"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..630
                     /locus_tag="PL106_07710"
     CDS             148..630
                     /locus_tag="PL106_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32993.1"
                     /translation="MKLPILLPCLILTVGVVNAQKTAEDLDAMVNNDIAALNAHFNGI
                     VRFAIDKKDRLVVDHLIDGTSQRTDVAYVDFLDAGTCAYSAEEQRMVLQCQDPRSKCI
                     EQGIKGKGDAVPTGRMSMALPGGDAKGQKAQELIVRLIEHKQEAELSRLAEANTRERK
                     "
     gene            641..1198
                     /locus_tag="PL106_07715"
     CDS             641..1198
                     /locus_tag="PL106_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA32994.1"
                     /translation="MQKRTIITFGAAWLLNGAWAQSNAQGTGDLALVPPDQETERLFH
                     EGEKAARSGDAAKAIGFYDRVLERDPNHLNAWLQRGASHIRLKDYATAVNDFTEVIER
                     KSDHQWAFTSRGCAYLKLGRNDEALRDLDTAIELDPKDQEAWNNRGWAYKAMGRMDTA
                     CRDWKTSKKMGNAEARIILENNHCK"
     gene            1195..1602
                     /locus_tag="PL106_07720"
     CDS             1195..1602
                     /locus_tag="PL106_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32995.1"
                     /translation="MTRKSLSFLLALSSMAALAQDRDAADCVMKIRSDWGQPCEKCES
                     YVESYKRDYRGVYRVELSNTCGNAIELKVAMQEANGRWRTFPVKALANGESMTAFACN
                     GTGKYLYWVRRLNDTEILLPSDQEILTEYRDRR"
     gene            complement(1615..>2316)
                     /locus_tag="PL106_07725"
     CDS             complement(1615..>2316)
                     /locus_tag="PL106_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32996.1"
                     /translation="GKRSKGAWTYGYEAYLTNGFDATIISNAEGHTFLPASRDNPDRF
                     TESANGVPLVSAKAAVRHERLGEVGISTMGGVFNQFKADGLTLDRRRRVDVVAIDFNT
                     VSQRWGTSVLGEWAWVVVDVPATYSQQFGTRQRGGFVDIVQPVLRRRVFGFNKAVLNL
                     ALRLGHVDHNVGRFKESGTVIGDEVLEIVPGLSFRPVPGTVIRLNYRIERAYDLFRDP
                     PARTGGFQFGVASYF"
BASE COUNT          515 a          705 c          703 g          393 t
ORIGIN      
        1 agcatgaggg caccggccat gatcaacgtt cgagcggcga cggatgaatt cttgctttcc
       61 atgtggtttg ggaactattg gcccttcgtt acgtaaacgc gccccgaaag ttcccggacc
      121 acatccccgg gccaacacca gaaccacatg aaacttccga tccttctccc ctgtttgatc
      181 ctgactgtcg gcgttgtgaa cgcgcaaaag acggccgagg acctggatgc catggtcaac
      241 aacgacatcg cggcgctcaa tgcgcatttc aacgggatcg ttcgattcgc gatcgacaag
      301 aaggacaggc tcgtggtgga tcacctgatc gatggcacga gccagcgcac cgatgtggcc
      361 tacgtcgatt tcctggatgc tggaacctgt gcgtacagcg ctgaagaaca gcgcatggta
      421 ctccagtgcc aggatccccg gtccaagtgc attgaacagg ggatcaaggg caagggcgat
      481 gcggtcccaa cgggccgtat gagcatggcc ctgccaggcg gcgatgccaa gggccagaag
      541 gcgcaggagc tgatcgttcg cctgatcgag cacaagcagg aggccgaact ctcgcgtctc
      601 gccgaggcca atacacgcga gcgcaagtaa gatggccttc atgcaaaaga gaaccatcat
      661 cactttcggg gccgcatggc tcctgaacgg tgcgtgggcg cagtccaatg cgcagggtac
      721 cggggatctc gccttggtcc cgcccgacca ggagacggaa cgcctcttcc acgaagggga
      781 gaaggcagcc cgctcaggtg atgccgcaaa agccatcggc ttctatgacc gggtcctcga
      841 acgtgacccg aaccacctga atgcctggct gcaacgcggt gcgagccata tccggctgaa
      901 ggactacgct accgcggtga acgacttcac ggaggtgatc gaacggaaga gcgaccacca
      961 atgggccttc accagccggg gttgtgcgta cctcaagctt ggcaggaacg acgaggccct
     1021 ccgcgatctg gacaccgcga tcgaactgga ccccaaggac caggaggcgt ggaacaaccg
     1081 tggatgggcg tacaaggcca tgggccggat ggacacggcc tgccgcgatt ggaagacgag
     1141 caagaaaatg ggcaacgccg aagcccgcat catcctggag aacaaccact gcaaatgacc
     1201 cggaaaagcc tttcttttct tcttgccctg tcgtccatgg ccgccttggc acaggatcgt
     1261 gatgccgccg attgcgtgat gaagatccgc agcgactggg gccagccatg cgagaagtgc
     1321 gaatcgtacg tggaatcgta caagcgcgac tatcgcggtg tgtaccgtgt ggagttgagc
     1381 aacacctgcg gcaatgccat cgaattgaag gtggccatgc aggaggccaa tggtcgctgg
     1441 cgcaccttcc cggtcaaggc gctcgccaac ggggaaagca tgaccgcctt cgcgtgcaac
     1501 ggcacgggca agtacctgta ctgggtgcgc aggctgaacg acacggagat cctgttgccc
     1561 agcgaccaag agatcctcac cgaatacagg gatcggcgtt gagaccggtc ggggtcagaa
     1621 atagctggcc acaccgaact ggaagccacc ggtgcgtgcc ggcgggtcgc ggaacaggtc
     1681 gtacgcgcgt tcgatacggt agttgaggcg gatcaccgtg ccgggcacgg gcctgaacga
     1741 gaggccggga acgatctcga gtacctcatc gccgatcacc gtccccgact ctttgaagcg
     1801 gccgacgttg tggtccacat ggcccaaacg cagcgccagg ttgagcacag ccttgttgaa
     1861 accgaacacg cgcctccgca acaccggctg aacaatgtcg acgaagccgc cccgttgacg
     1921 tgtgccgaac tgctgcgaat aagtggccgg aacatccacc accacccagg cccactcgcc
     1981 caaaacagaa gtgccccatc gctgcgaaac ggtattgaaa tcgatagcga ccacatctac
     2041 gcgccgacgc cggtcgagcg tcagaccgtc cgccttgaac tggttgaaca ccccccccat
     2101 ggtgctgatg ccgacctcgc ccagccgttc gtgacgtacg gccgccttcg ccgagaccaa
     2161 tggcacccca ttggcgctct ccgtgaagcg atccgggtta tcccttgatg ccggcaaaaa
     2221 tgtatggcct tcggcattgc tgatgatggt ggcatcgaag ccgttggtca ggtaagcctc
     2281 gtacccgtag gtccatgcgc ctttggagcg cttgcc
//
LOCUS       DAOQOC010000434         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28463_length_2313_cov_4.589903, whole genome shotgun sequence.
ACCESSION   DAOQOC010000434 DAOQOC010000000
VERSION     DAOQOC010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28463_length_2313_cov_4.589903"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PL106_07730"
     CDS             <1..770
                     /locus_tag="PL106_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA32997.1"
                     /translation="VMLDTAVANGPYTISVSAAACPVAPANDACSGAIALTAGVDCIG
                     TPFTTMGATQDMEPIECGGYTSTNANDVFFTFVATATDMTVGVTGFNQADPMVELFEG
                     SCASPSSLACADATFPQSAGQTTSEQLIQSGLTVGTTYIVRVYDWGHTSPEHNFEICV
                     TEGSGNNIGIEENPETSFSLYPNPGTGIFSLSYSGSNGLANIEVMDVAGRVAHSVRER
                     VSKGSVLPLDLSAMSAGNYSVRLTVNGVRTEQRLMVK"
     gene            complement(835..1623)
                     /locus_tag="PL106_07735"
     CDS             complement(835..1623)
                     /locus_tag="PL106_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA32998.1"
                     /translation="MDLSLIGNHALVCGASQGIGRAAALELARLGASVTLLARDGDKL
                     RAVLAELDRTAGQGHATLEVDMSVTEQLRAAMEALLAKHGPVHILVNNSGGPTPGLAH
                     ESAVNAFAGAFRQHLLAFQTMVLALVPGMKDAGHGRIINVISTSVKQPLPNLGVSNTV
                     RGAVAQWSKTLASELGPFGITVNNVLPGATRTERLATIIRNRVAKTGQSEEEVAHEMT
                     REVPLRRFAEPWEIAAAIAFLAGPSAAYINGINLPVDGGRTGCL"
     gene            1724..>2313
                     /locus_tag="PL106_07740"
     CDS             1724..>2313
                     /locus_tag="PL106_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA32999.1"
                     /translation="MRDHLLFVIAACAALSASAQQFPSHTPGIGPLTMTASAWPRSHG
                     THDGGVRGGAPYNDLCENAVIHPLEQGTPVTILGDNTGSTDTENFGNPVSWEAFSIGI
                     CATVTLDYCGTDPLFEFVYSILITGCPDFVASVQNNGTSFCDDGNTTIVYENLPPGIY
                     YIPVLRMTGAVGPYTIHVSAGPCDGPPLNDVCANAQ"
BASE COUNT          421 a          743 c          719 g          430 t
ORIGIN      
        1 ccgtgatgct cgatacggcc gtagcgaacg ggccgtacac gatcagcgtt tctgcggcgg
       61 cctgcccggt ggcaccggcc aacgatgcct gctccggagc catcgcgctg accgctggcg
      121 tggactgcat cgggactccc ttcaccacaa tgggggccac gcaggacatg gagccgatcg
      181 agtgcggagg atacacctcg accaacgcga atgatgtgtt cttcaccttt gtggctaccg
      241 ccacggacat gaccgtgggg gtcaccggtt tcaaccaggc cgatcccatg gtggaattgt
      301 tcgaggggtc ctgcgcttcg cccagctcgc tggcctgtgc ggacgccact ttcccgcaat
      361 ccgccgggca gaccaccagc gaacaactta tccaaagcgg actcacggtg ggcaccacgt
      421 acatcgtgcg cgtctatgat tggggccata cgtcccccga gcataacttc gagatctgtg
      481 tcaccgaggg gagtggcaac aacatcggca tcgaggagaa ccccgagacc tcattcagcc
      541 tctacccgaa ccccggtacg ggcattttca gcttgagcta cagcggcagc aacgggctgg
      601 cgaacattga ggtgatggat gtggccggcc gggtggcgca tagcgtacgc gagcgcgtga
      661 gcaagggctc cgtgcttcct ttggacctct cggcaatgag cgccggcaac tacagcgtgc
      721 gtctcacggt gaacggtgtt cgtacggaac agcgcctgat ggtgaagtag gagcgatacc
      781 ttttattagg gagaagggcg ggcagcgatg tccgcccttc ctcgttctcc ggcatcagag
      841 gcagccggtc cgtcctccat ccacgggcag gttgatcccg ttgatgtacg cggcactggg
      901 cccggccagg aaggcgatgg cggcggcaat ttcccatggc tcggcgaagc ggcgcaacgg
      961 cacctcccgg gtcatctcat gggccacctc ctcctcgctc tggccggtct tcgcaacgcg
     1021 gttccggatg atggtggcca ggcgctcggt acgggtggcc ccgggcagca cattgttcac
     1081 tgtgataccg aacggcccca attcgctggc cagtgtcttg ctccattggg ccacagcacc
     1141 ccgcactgta ttgctgacgc ccaggttcgg cagcggctgc ttcacactgg tgctgatcac
     1201 gttgatgatg cgaccatggc ccgcgtcctt catccccgga accaaggcga gcaccatggt
     1261 ctggaacgcg agcagatgct ggcggaacgc acccgcgaaa gcgttcacag cactctcatg
     1321 cgccaggccg ggcgtggggc cgccgctgtt gttcaccagg atatgtaccg ggccgtgttt
     1381 ggccaacagc gcttccatcg cggcgcgcaa ctgctccgtc accgacatgt ccacctccaa
     1441 ggtcgcgtga ccctgcccgg cggtgcggtc caattcagcg agcaccgcgc gcagcttgtc
     1501 cccatcgcgc gccagcaacg tgacgcttgc accgaggcgg gccagttcca gtgctgcggc
     1561 cctgccgatg ccttggctgg caccgcagac cagggcgtgg tttccgatca acgagaggtc
     1621 catgatgcaa cgttttcctg ccggggatgt ggacggagga gcggtaaaag taggggcgtg
     1681 tttcccgtgc gatcggcacc tttgcgtgaa agaaccggtg atcatgcggg accatttgct
     1741 cttcgtcatt gcggcttgtg cggcgctgag cgcgtccgca caacagttcc cctcccacac
     1801 tccagggatc gggccgctca ccatgaccgc atcggcatgg ccacgctccc atggaacgca
     1861 cgatgggggc gtgcgtggtg gagcaccgta caacgacctc tgcgagaacg ctgtgatcca
     1921 cccgctcgag cagggtaccc cagtgaccat cctcggcgac aacacgggtt ccacggatac
     1981 ggagaacttc ggcaatccgg tgagctggga ggccttcagc atcgggatct gcgccaccgt
     2041 caccttggac tactgcggta cggacccgct gttcgagttc gtttattcga tcctgatcac
     2101 gggttgcccg gatttcgtgg ccagcgtgca gaacaacggc acgtcgttct gcgacgacgg
     2161 caacacgacc atcgtgtatg agaaccttcc gccgggtatt tattacatcc ccgtactgcg
     2221 catgaccggg gccgtgggac cgtacacgat ccatgtctcg gccgggccct gcgacggacc
     2281 accactcaac gatgtgtgcg cgaacgcaca aga
//
LOCUS       DAOQOC010000435         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28469_length_2313_cov_3.746236, whole genome shotgun sequence.
ACCESSION   DAOQOC010000435 DAOQOC010000000
VERSION     DAOQOC010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28469_length_2313_cov_3.746236"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PL106_07745"
     CDS             <1..267
                     /locus_tag="PL106_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33000.1"
                     /translation="WALLTREFIRSRGCLGSRMHRDKHGRLVAIAIWPDRAAWEAKPT
                     DPAPELVAARQTMMKACELFRTEAELETVMDLWVNGLPDRSSPG"
     gene            423..587
                     /locus_tag="PL106_07750"
     CDS             423..587
                     /locus_tag="PL106_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33001.1"
                     /translation="MSSRKKNLILTAPEPKAGTKFVKVRLDGRTIVHLKSMKAFPFWK
                     SRYPKAEVID"
     gene            1174..1665
                     /locus_tag="PL106_07755"
     CDS             1174..1665
                     /locus_tag="PL106_07755"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNA33002.1"
                     /translation="MPSVDIVSKVDAQTLDNAINSAKRELDNRYDLRGSNSTIEYDRK
                     ALTIKVSTEDHLKLDAIVDILLERSTKQKIDVKSFDLKEEPQPSGKLLYKLIKVQNGI
                     EREKAKKIIKAIKETKLKVEPQIQDDHIRVSGKKIDDLQAVMAMCRTTDFEVPLQFDN
                     MKS"
     gene            1719..>2313
                     /locus_tag="PL106_07760"
     CDS             1719..>2313
                     /locus_tag="PL106_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33003.1"
                     /translation="MRPRSRSGHLSSLLFGLLTFATADAQTYYYLDQISVSPATPTTA
                     DAITITLNGSLSNTASYIVNTDFSIQGTTVMITVNAAMSGIGLDVLVPHSETLPVGNL
                     AAGTYTIVITGTATLDMAAVPEHQFTVSAGEPTDCDSLSFLGVRWGVFSDSTIVLTVA
                     NASTDLFDYPGFVLLDEEGDTIASETVSYFGIGSGPQD"
BASE COUNT          555 a          697 c          631 g          430 t
ORIGIN      
        1 tgggccttgc tcacccgcga attcatcagg agccggggtt gtttgggcag caggatgcac
       61 cgcgacaagc atggccggtt ggtggccatc gccatctggc ccgatcgagc tgcttgggag
      121 gccaaaccca cggaccccgc acccgagttg gtcgccgccc gccaaacgat gatgaaggca
      181 tgtgaactgt tccgtacgga ggcggagctt gaaactgtta tggacctctg ggtgaatggg
      241 ctccccgatc ggagcagccc cggctgagcc cgtcccatca aggccttaac gatcattgga
      301 atgtcaaacc cgtgtcagga ctggcatagc atggtcattc gggttacatt cgtccgccat
      361 tcaactggaa cgccttccgg gatcaccgcg gtcgcgtaca gccgaacaga gcagagagaa
      421 ccatgagcag ccgcaagaag aacctgatcc tcaccgcccc agaacccaag gcaggcacca
      481 aattcgtcaa ggtgcgactc gatggtagga ccatcgttca cctgaagagc atgaaagctt
      541 ttccattctg gaagagccgc taccccaaag cggaggtgat cgactgagca cccactgggg
      601 atatcgccct gagggcgaga aagttgcact acgacgtcgc agcagcgtgt ttggtggccc
      661 agttcggtag gtccggcacc ctcatcgtgt tcgtgaagac cgtcggaaca aggacggcca
      721 cccctcgagt cccctgattt ccctcgtgta aatcggtcca tttcaacacg agatgaacta
      781 cggtcagata gccggatctg tattcgcgtt ggtgggttac ctgggacgga tgagaagccg
      841 gttaggcgac caaatgatgg ctcgggttgg gaaatttgct tggctggcca aacaagcatc
      901 cggtcaatct gagctcctaa tgttgccaat aagcatcgca acggtctcct cgtggacatt
      961 aaatgcgaca tatccaactg gatatcacta taataacgct gattgtttgg ccagccaagc
     1021 aaaatattac gccatcatga acgcgaaacc tgctcggccc catccataaa gatcatctga
     1081 ccaccccgcg cacgacccac cggatcgggc catcccgatc ggccgaccac cagtcaccca
     1141 ccggctcgta cacttgcgca agaacaaaac gccatgcctt cagttgacat cgtttccaag
     1201 gtggacgcgc agacactcga caacgcgatc aattccgcca aacgggagtt ggacaaccgg
     1261 tacgacttgc gcggcagcaa cagcaccatc gagtacgatc ggaaagcgct gaccatcaag
     1321 gtgtccacag aggatcatct caaattggac gccatcgtgg acattctgct ggagcgcagc
     1381 accaaacaga agatcgatgt gaaaagcttt gacctgaagg aagaacccca gccgagcggc
     1441 aagctgttgt acaagctgat caaggtgcag aacggcatcg agcgcgaaaa ggccaagaag
     1501 atcatcaaag cgatcaagga gaccaaactc aaggtggaac cgcagatcca ggacgaccac
     1561 atccgtgtga gtggcaagaa gatcgacgat ctgcaggccg tgatggccat gtgccgtacc
     1621 acggactttg aggtgccgct ccagttcgac aacatgaaaa gctgatcgcc gggcagcgcg
     1681 ctgggcttcc cgtgcgtcat ttcatcaaac ctcgccagat gcgcccacga tcccgctcag
     1741 gacatctgtc ctccttgctt ttcggcctgc tcacgttcgc cacggccgat gcgcagacct
     1801 attactacct ggaccagata tcggtctccc ctgcgacccc caccacggcc gatgcgatca
     1861 ccatcacatt gaacggctca ctctccaata cggcgtcgta catcgtgaac acggacttca
     1921 gcatccaggg taccaccgta atgatcaccg tgaatgccgc catgagtggg atcggattgg
     1981 atgtcctcgt tccacacagc gagacactgc ccgttggcaa cctcgcggca ggtacgtaca
     2041 ccatcgtcat caccggaacg gccacgctgg atatggctgc cgtgccggaa caccaattca
     2101 ccgtgagtgc cggtgaaccg acggactgcg atagcctttc attcctcggc gtccgctggg
     2161 gggtattcag cgacagcacc atcgtgctca cggtggccaa cgccagcacc gacctgttcg
     2221 attacccagg attcgtactg ctggatgagg agggagacac catcgcctcg gagacggtga
     2281 gctacttcgg gatcggttcc gggccacagg acc
//
LOCUS       DAOQOC010000436         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28513_length_2312_cov_2.400089, whole genome shotgun sequence.
ACCESSION   DAOQOC010000436 DAOQOC010000000
VERSION     DAOQOC010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28513_length_2312_cov_2.400089"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PL106_07765"
     CDS             <1..422
                     /locus_tag="PL106_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33004.1"
                     /translation="KSGMEPAVGLVDQELEDARQSSYPFPELAYRELDYGAVLNGVVE
                     IDGRKCDRIFVKKAAGGVFTEYYDQATGLKIRRTETQNAPQGTMQVTTDFKDYKEHDG
                     ILFPETIEQDLGANVRFAARTIEVNGSIPAAEFKMMD"
     gene            complement(434..2095)
                     /locus_tag="PL106_07770"
     CDS             complement(434..2095)
                     /locus_tag="PL106_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33005.1"
                     /translation="MAHHALLLVTFPVICLSAIAQTPFEQADQRTPHTGYSHAVVMGS
                     GWAVAGSAYPDDVWGGGAFLLGIGPDGDVMWEHPVASGFLMSGVTDLATAPNGDIVSA
                     STLIDCDVLMPYFLTERTAPDGSTVWSRTCPFDNVTALAVAENGDIAFSTGTGILITD
                     ADGDSLNWFGTPDTYSMDLMWDGDSSLLTNRWDGILARWSYHGGFLAADTVAGSITDM
                     AHWHGHRLVLTTDGVLRKLNEVLSVTDSMDLGDGFAKGRLLPLTDRLLVLGNEHMTEL
                     DTALAVTSMIPVDPDNEFPADLFSAFAGNDSMVLMTSTSGVGGISAGLVRSLVQNGDH
                     EPHGENVGITILAIDSVYYHLQNGIVQPQVDVTVRVTNLGPTVVNDVLVAHKGINGIA
                     CGYYGTSQHVHNAGLAPGAAMDIPLPGLWLYYAPWDFVTLDQPVCIAALSPNDLYDRD
                     HSDNYACDTAHIVLGIDDVTVTPVFVVTNPISDAIDMRFPEPRATPQQVELLDATGRR
                     VGTTLVPAGSGSYHWPLPDLHNGVYFLRMEYSGAMTAHKLLRQGD"
BASE COUNT          466 a          707 c          698 g          441 t
ORIGIN      
        1 agaagtcggg catggagccc gctgtggggc tggtggatca ggaactggag gatgcccgcc
       61 aaagttccta tccctttcct gaactcgcct accgggaact cgactacggc gctgtgctga
      121 acggtgtggt ggagatcgac ggccgcaagt gcgaccggat cttcgtgaaa aaggccgctg
      181 gtggcgtctt caccgagtat tacgaccagg ccaccgggct gaaaattcgt cgcacagaga
      241 cccagaacgc accgcagggc accatgcagg tgaccactga tttcaaggat tacaaggaac
      301 acgatggcat cctcttcccc gagaccatcg aacaggacct tggtgccaac gtgcgcttcg
      361 ctgcgcggac catcgaggtg aacggatcga tcccggcggc ggagttcaag atgatggact
      421 gacccctccg gactcaatca ccctgtcgga gcaatttgtg cgcagtcatg gcgccgctgt
      481 actccatgcg caagaagtac accccgttgt gcaggtccgg caggggccag tggtagctcc
      541 cgcttcccgc tggcaccaag gtcgtgccca cccgccgtcc ggtcgcatcc aatagttcca
      601 cctgttgcgg cgtggccctc ggttcaggga accgcatgtc gatcgcgtcc gagatggggt
      661 tggtcaccac aaataccgga gtaacggtga catcatcaat gccaagtaca atatgtgccg
      721 tgtcgcaggc gtagttgtcg ctgtggtcgc ggtcgtacag gtcgttgggg ctgagggcag
      781 cgatgcatac gggctggtcc agtgtgacga agtcccaggg tgcgtaatag agccatagac
      841 cgggcaatgg gatgtccatc gccgccccgg gggccagacc ggcgttgtgt acatgttgcg
      901 atgtaccgta gtagccgcac gcgatcccgt tgatcccctt gtgcgccacc agcacatcat
      961 tcactacggt cggaccaagg ttggtcacac gcacagtgac atcgacctgc ggttgaacga
     1021 tcccattctg caggtgatag tacacgctgt cgatcgcaag gatggtgatg cccacgttct
     1081 caccatgcgg ttcgtgatcc ccattctgta caaggctgcg caccaacccc gcactgatcc
     1141 ctccgacccc ggaagtggag gtcatgagta ccatgctgtc attgcccgca aatgcgctga
     1201 agaggtccgc cgggaactca ttatccgggt ccactggtat cattgatgtg accgccagag
     1261 ctgtgtccag ttccgtcatg tgctcgttgc caagaacgag caggcgatca gtcaaaggaa
     1321 ggagacgccc cttcgcgaac ccatcgccaa ggtccatgct gtccgtcact gaaagcacct
     1381 cattcaactt gcggaggacc ccgtccgtgg tcagtaccag gcgatggccg tgccagtgcg
     1441 ccatgtcggt gatgctcccc gctacggtat ccgcggccag gaacccgcca tggtacgacc
     1501 agcgcgcaag tatgccgtcc catcggttgg tgagcaaact ggagtcccca tcccacatga
     1561 gatccatgga gtatgtgtcc ggcgtaccga accagttcag gctgtcgcca tctgcatcgg
     1621 tgatcaagat cccggtaccg gtggagaagg cgatgtcgcc gttctcggct accgccaacg
     1681 cggtgacatt gtcgaatgga caggtcctgg accagacggt gctgccatcc ggcgctgtac
     1741 gctcggtgag gaagtagggc atgagcacat cacaatcgat gagcgtgctg gccgaaacga
     1801 tgtccccgtt cggcgcagtg gccagatcgg ttacgccgct catcaggaat ccggatgcca
     1861 ccgggtgctc ccacatcacg tccccatccg gcccgatccc gagcaggaag gccccgccac
     1921 cccacacgtc atccgggtac gcgctgcctg ccacggccca gccggagccc atgaccacag
     1981 catgcgagta gcccgtatgc ggcgtccgct ggtccgcttg ttcgaaaggg gtttgggcga
     2041 tcgcggaaag acagatgacc gggaacgtga ccaggaggag tgcatggtgc gccatgatcc
     2101 gaaggtgttt ttcatcagac atgccagcac atgtgagtgc tgcctggtgc ggatgttgtc
     2161 atgcgaaagg ggccatccct cgcggaacgg ccccttgctc tctcatctga tgcgcttact
     2221 ggatcaccag gcgctgtgtg cgaagcgttc caccggcgcg cacattgacg aggtacagcc
     2281 caggggccag atcccgctcg aagctcatgc tc
//
LOCUS       DAOQOC010000437         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28677_length_2305_cov_2.447556, whole genome shotgun sequence.
ACCESSION   DAOQOC010000437 DAOQOC010000000
VERSION     DAOQOC010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28677_length_2305_cov_2.447556"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2305
                     /locus_tag="PL106_07775"
     CDS             <1..>2305
                     /locus_tag="PL106_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33006.1"
                     /translation="NASPTANTTYTVTGMDGEGCTNTATATVNAINAPVVNSATADPN
                     PACFNGTSVLNATAILPSTVDQYVFASSVGTFTSIVGAPGTVQINTGADDTGYGPYPL
                     GFTFNFNGINFTSAGVNANGYVAMGALASSSYTALSSGSLNNVIAAFNGDLYGLSANG
                     AKMSYQLSGTPGSQVFTFEWTNWGFYSSGLNEASYQVKLYEGSNTVQIVYQNGTGTNA
                     KNVQVGLRGSSTAVFSNRTTTTDWTATTAGGANTATCTVSATVRPVNGLTFTWTLPSL
                     LGATYAWTPNTFLDDASLQSPTASGVNVASIGYQVTATYLGCPSSNFGAVTLTTSAPI
                     TAATITGTLEYCAGGSTTLTAVPTDGAGPYTYLWSPGGETTASIDASAPGLYNCQVTD
                     GCGGSVNTGDVTVTELAIPTVEVSPTSALLCDGASQVLTASGADSYAWSPAAGLDMVT
                     GPTVTATPATSTTYTVTGTAANGCTNTATAAMSVGTTPVVNNVSATPGTACYNSDVQL
                     LASAGTPPSYCNSNFTSATFEFITNVDYAGISQTSGGNIGGPVDYTAQVASVTVGTPN
                     NLSVTVDPDASDYVYAWIDWNQNGVLNDAGETYTLATSTSLPGPHILSITPPAGAYNG
                     NTRMRVMIDYANAVPNPCRSATWGEAEDYTVNVSGGVEQYLYSWTPATGLDDATIPNP
                     MALDVTVDASYTVTVSDGAGCAAASQTASFTVLGPVTTPVIAPAAATICVGGDVQLTA
                     SVPGSEPAPYCLSNFTSSTFESLANVT"
BASE COUNT          463 a          773 c          674 g          395 t
ORIGIN      
        1 tgaacgcgag cccgacggcc aacaccacct acaccgtgac cggtatggat ggcgaaggct
       61 gcacgaacac ggccaccgcc acggtgaatg cgatcaatgc acccgtggta aatagtgcaa
      121 cagccgatcc gaaccccgcc tgcttcaacg gcacctccgt actgaatgca acggcgatcc
      181 tgccaagcac ggtggaccag tacgttttcg ccagctcggt aggcaccttc acatccatcg
      241 tgggggctcc cggcaccgtg cagatcaata cgggagcgga tgacacgggt tacggcccgt
      301 atcctctcgg cttcaccttc aacttcaacg ggatcaactt caccagtgcc ggcgtgaacg
      361 ccaacggcta cgtggccatg ggcgcactgg catcgagttc ctacaccgcg ttgagcagcg
      421 ggtcgctgaa caacgtgatc gccgcgttca acggggacct gtacggtctg tccgccaacg
      481 gcgccaagat gagctaccag ttgtccggca cgcccgggag ccaggtgttc accttcgaat
      541 ggaccaactg gggtttctat tcctcgggtc tcaacgaagc gagctaccag gtcaagttgt
      601 acgagggttc caacaccgta cagatcgttt accagaacgg cacgggaaca aacgcgaaga
      661 acgtacaagt aggtctgcgt ggatcctcaa ccgcggtgtt ctccaaccgc accaccacca
      721 cggattggac ggcgaccacg gcgggcggtg ccaacacggc cacctgcacg gtgagcgcca
      781 cggtacgccc ggtgaacggt ctcaccttca cgtggaccct cccttctctc cttggcgcca
      841 cgtacgcgtg gaccccgaac accttcctgg atgacgcttc gctgcaaagc cccacggcca
      901 gcggggtcaa tgtggcgagc atcggatacc aggtaacggc cacctacttg ggatgcccat
      961 cttccaactt cggtgcggtc acgttgacca cgagtgctcc gatcacggcg gccacgatca
     1021 cgggcacctt ggaatactgc gctggtggta gcaccacctt gacggccgta cccaccgatg
     1081 gtgccggccc gtacacctac ctgtggagcc ccggtggcga aaccacggca agcatcgacg
     1141 cgagcgcccc cggcctgtac aactgccagg tgaccgatgg ctgcggcgga tcggtgaaca
     1201 ccggtgatgt caccgtgacc gaactcgcca tccccaccgt ggaggtgagc cccaccagcg
     1261 cgctgctgtg cgatggtgca agtcaggttc ttaccgccag tggtgcggac agctacgcgt
     1321 ggtcgcccgc tgctggcctc gatatggtta ccggtcctac ggtaacggcc acccccgcca
     1381 cgagcaccac ttacacggtg accggcacgg cggccaacgg ctgcaccaac acggcaacgg
     1441 ccgccatgag cgttggcacc acaccggtgg tgaacaacgt ttcggccaca cccggcaccg
     1501 cctgctacaa cagcgatgtg caactgctgg cttcggctgg cacgcccccg agctattgca
     1561 acagcaactt cacgtcggcg acctttgagt tcatcaccaa cgtggactat gcgggcatca
     1621 gccagacctc cggtgggaat atcggcggcc cggttgatta caccgcccag gtggcgagtg
     1681 taacggtggg taccccgaac aacctgtcgg tcaccgtgga tccggatgcg agcgactacg
     1741 tgtacgcttg gatcgattgg aaccagaacg gcgtgctgaa cgatgcgggc gagacctaca
     1801 ccctggccac gagcaccagc ttgccaggcc cgcatatcct gagcatcact ccgcctgccg
     1861 gtgcctacaa cgggaacacc cgcatgcgtg tgatgatcga ttatgccaac gccgttccga
     1921 acccctgccg cagcgccact tggggcgaag cggaggacta cacggtgaac gtgagcggtg
     1981 gtgttgagca atacctctat agctggaccc cggccactgg cctggacgat gccaccatcc
     2041 ccaaccccat ggccttggac gttacggtgg atgccagcta caccgttacg gtgagcgacg
     2101 gggccggatg tgcggcggcg agccaaacag cttccttcac cgtgctgggt ccggtgacca
     2161 caccggtgat cgcacctgct gcggccacga tctgtgtggg gggcgatgtg caactgacgg
     2221 cctcggtacc cggtagcgaa ccggcgccct actgcctcag caacttcact tcttcgacct
     2281 ttgagtcgct cgccaacgtc acctt
//
LOCUS       DAOQOC010000438         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28745_length_2302_cov_3.638184, whole genome shotgun sequence.
ACCESSION   DAOQOC010000438 DAOQOC010000000
VERSION     DAOQOC010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28745_length_2302_cov_3.638184"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PL106_07780"
     CDS             complement(<1..871)
                     /locus_tag="PL106_07780"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNA33007.1"
                     /translation="MLRAIILFPTLLTALCGMGRVCEVYSGGSIEQALATTMDCDTVR
                     VHAGVYNEGPITITRPVVLLGEGWPVIDGGGKGEVIVVKAPHVTIRGLVVRGTAISDI
                     NDNAAIKCISVTDITIAGNRIEDSFFGIYLSSVARASVSDNRVIGDAATPEGRRANGI
                     HLWKCAHVSVERNTVMHHRDGIYLEFVTDSRIEGDTSAYNSRYGLHFMFSHRDRYMRN
                     VFRNNGAGVAVMFTHDVIMADNHFEENRGASSYGLLLKEINDATITGNVFADNTVGIL
                     IDGCNRAQVKDNLF"
     gene            complement(875..1951)
                     /locus_tag="PL106_07785"
     CDS             complement(875..1951)
                     /locus_tag="PL106_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNA33008.1"
                     /translation="MTPVPRLRIITRILIALAALSLGLLLKVPIWRIDMMAPQYPEGL
                     ALQIFHDRFTGDVDKINGLNHYIGMATIHNDMFPEFAIMRYAFYLLIGWGLVVTMIGR
                     RGGLFTWLLVLFAYVIWAMWDMYSWGYRYGHNLDPHAAIKVEGMAYQPPLFGHKKLLN
                     FDAWSLPDTGGWILFSVISLACILWFIEWRWPRRVGPRPEPVEKPRPTDVPKTQVLAA
                     MLAVLFSAGCAGPSTPTVDFGMAECAYCRMNVVDRQFASAIITGGGRTYAFDSPECMV
                     QHVAEGAIAEPQVDSWWVCDHAHPGTFIDATKALYAHAPTLKSPMNGNTAAFAFEADR
                     AAILKDHPGDALDWKAARTLLTHH"
     gene            complement(2027..>2302)
                     /locus_tag="PL106_07790"
     CDS             complement(2027..>2302)
                     /locus_tag="PL106_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HNA33009.1"
                     /translation="DEVYFHVTNLEQDWDVPHGFAMKGAPTAELLIMPGETQTLKWVP
                     QSVGVKPFYCTDFCSALHQEMQGYLRVSPAGSNVPLKFETRSVDAGS"
BASE COUNT          437 a          700 c          720 g          445 t
ORIGIN      
        1 ggaagagatt gtccttcact tgcgcgcgat tgcatccgtc gatcaggatg cccaccgtgt
       61 tgtcagcgaa cacgttgccg gtgatggtcg catcattgat ctctttcagc agcaatccgt
      121 atgagctggc tccacggttc tcctcgaaat ggttgtcggc catgatcaca tcgtgggtga
      181 acatcacggc cacaccagcg ccattgttgc ggaacacgtt gcgcatgtag cggtcgcgat
      241 ggctgaacat gaagtggagg ccgtaccggg agttgtacgc gctggtgtcc ccctcgatcc
      301 gcgaatcggt aacgaattcc aggtagatac cgtcgcggtg gtgcatcacg gtgttgcgct
      361 ccaccgaaac atgtgcgcat ttccacaggt ggatgccgtt ggcccgacgg ccttccggcg
      421 ttgccgcatc gccgatcacg cggttgtcgg agaccgatgc gcgcgccacg ctgctcaggt
      481 agatcccgaa gaaactgtcc tcgatccggt ttcccgcgat ggtgatgtcc gtgacgctga
      541 tgcacttgat cgcggcattg tcgttgatgt cgctgatggc cgtgccacgg accaccagcc
      601 cccggatggt cacgtgcggg gccttcacca cgatcacctc acccttgccg ccgccatcga
      661 tcacgggcca gccttcgccg agcagcacca cgggtcgtgt gatggtgatg ggcccttcat
      721 tgtacacccc ggcatgcaca cgcaccgtat cacagtccat ggtggtggcg agtgcttgct
      781 cgatggaacc gccgctgtac acttcgcata cacgccccat tccgcacagt gcggtgagga
      841 gggtggggaa aagaatgatc gcgcgaagca tagatcaatg atgggtgagc agcgtgcgtg
      901 cggccttcca atcgagcgcg tcaccgggat ggtccttcag gatcgcagcg cggtccgctt
      961 cgaaggcgaa cgccgccgtg ttgccgttca tgggactctt caaggtgggc gcgtgcgcgt
     1021 acagggcctt ggtggcgtcg atgaaggtgc ccggatgcgc gtggtcgcac acccaccagc
     1081 tgtccacctg cggttcggcg atggctcctt cggccacgtg ctgcaccatg cactccggcg
     1141 aatcgaacgc ataggtgcgc ccgcccccgg tgatgatcgc ggaagcgaat tgccggtcca
     1201 ccacgttcat gcggcagtag gcgcattccg ccatgccgaa atcgacggtc ggcgtgcttg
     1261 ggcccgcgca tccggcggaa aataataccg caagcatagc ggccaggacc tgcgtcttcg
     1321 gaacatcggt cggtcgcggc ttttccaccg gctcaggccg cggccccacc ctgcggggcc
     1381 agcgccattc aatgaaccag aggatgcagg ccaggctgat caccgagaac aggatccatc
     1441 ctccggtatc cggcagcgac caggcatcga agttcaagag cttcttgtga ccgaaaagcg
     1501 gtggctggta ggccatgcct tccaccttga tggccgcatg cggatccaaa ttgtgtccgt
     1561 accggtaacc ccaggaatac atgtcccaca tggcccagat cacgtacgcg aagaggacca
     1621 gcagccatgt gaacaggcca ccacggcgcc cgatcatggt taccacgaga ccccatccga
     1681 tgagcaggta gaacgcgtag cgcatgatgg cgaattccgg gaacatgtcg ttgtggatcg
     1741 tggccatgcc gatgtaatgg ttcaacccgt tgatcttgtc cacatcgccg gtgaaccggt
     1801 cgtggaagat ctgcagggca agaccttcgg ggtattgcgg ggccatcatg tcgatccgcc
     1861 agatgggtac cttcagcagg agtccgaggg acagcgccgc aagtgcgatg aggatccggg
     1921 tgatgatacg caggcgcggt acaggggtca ttggaggggg tgtttgcgac aacggcgggc
     1981 attgcgcccg ccgttgccac gattgataca accggtacgg gcgtggctac gagcccgcgt
     2041 ccacggaacg ggtctcgaac ttcagcggaa cgttgctgcc cgctggggat acgcggaggt
     2101 atccctgcat ctcctggtga agcgcgctgc agaagtccgt gcagtagaag ggtttcacac
     2161 ccacgctctg cggcacccac ttcaaggtct gcgtctcacc cggcatgatc aggagttcgg
     2221 ccgtgggcgc acccttcatg gcgaagccgt gcggcacgtc ccagtcctgc tccaggttgg
     2281 tcacatggaa gtacacctcg tc
//
LOCUS       DAOQOC010000439         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28773_length_2301_cov_3.630009, whole genome shotgun sequence.
ACCESSION   DAOQOC010000439 DAOQOC010000000
VERSION     DAOQOC010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28773_length_2301_cov_3.630009"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /gene="ccoN"
                     /locus_tag="PL106_07795"
     CDS             complement(<1..1517)
                     /gene="ccoN"
                     /locus_tag="PL106_07795"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNA33010.1"
                     /translation="MTERFRYDNRIAKDFLFITIFWGAVGMLVGLIAALELVIPDLNF
                     AKWISFGRIRPLHTNAVIFAFVGNAFFAGAYYSMQRLLKTRMYSDVLSRIHFWGWQLI
                     ILSAAITLPLGMTQGKEYAELEWPIDIAITIVWVVFGINMMGTILKRRERHLYVAIWF
                     YIATWVTVAMLHIVNSISIPVTLGKSYSWYAGVQDALVQWWYGHNAVAFFLTTPFLGL
                     MYYFLPKAANRPVYSYKLSIIHFWSLIFIYIWAGPHHLLYSALPEWAQSLGVVFSVML
                     IAPSWGGMLNGLLTLRGAWDRVREDPVLKFFVVAVTCYGMATLEGPLLSIKSINAIAH
                     YTDWIVAHVHIGGIGWNGFLTFGMVYWMVPRLFGNKLYSTKLANAHFWLGTLGIIFYA
                     VPLYFAGFFQSLMWQQFTPDGFLVYKNFLDTLLEIKYAYWLRAMGGTIYLVGVFIMVY
                     NVVKTVKAGRLIADEDAEAAALEKLDKEPGGHWHTWIERRPVQMLVWSLVAVAIGG"
     gene            complement(1514..1675)
                     /gene="ccoS"
                     /locus_tag="PL106_07800"
     CDS             complement(1514..1675)
                     /gene="ccoS"
                     /locus_tag="PL106_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNA33011.1"
                     /translation="MSVIILLLLVSTSVAGLFLLGFIHAVRRGQFDDDRSPAVRILHE
                     DDPRQTKTP"
     gene            complement(1807..>2301)
                     /locus_tag="PL106_07805"
     CDS             complement(1807..>2301)
                     /locus_tag="PL106_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNA33012.1"
                     /translation="ERMPLALLTGDSQVDPGLSDAFGAGSIRTGCSPMDKQRGIHEAQ
                     GRGRCVLMVGDGLNDAGALAQADVGITVTETSAALTPASDAIMDGPAIAGLPHFLRMA
                     RRARRIVIASLCISLGYNIIGVSLAVAGHMTPLIAAILMPLSSISVVSFVTAATAIGA
                     RELR"
BASE COUNT          468 a          719 c          702 g          412 t
ORIGIN      
        1 ccgccgatag ccacggccac caggctccag accagcatct gcacgggccg gcgttcgatc
       61 cacgtgtgcc agtggccccc gggctccttg tccagcttct ccaacgctgc cgcttcggcg
      121 tcctcgtcgg cgatcaaccg cccagccttc acggtcttca ccacgttgta caccatgatg
      181 aacaccccga ccaggtagat ggttccgccc atcgcgcgca gccagtacgc gtacttgatc
      241 tcgagcaggg tatccaggaa gttcttgtac accaagaagc cgtccggcgt gaactgttgc
      301 cacatcagac tctggaaaaa gcccgcgaaa tagagcggca cggcgtagaa gatgatgccc
      361 agcgtaccca gccagaagtg cgcgttcgcc agtttggtgc tgtacagctt gttaccgaac
      421 aagcggggta ccatccagta caccataccg aaggtgagga agccgttcca gccgatgcca
      481 ccgatgtgca cgtgcgccac gatccagtcg gtgtagtgcg cgatggcgtt gatgctcttg
      541 atgctgagca gcggcccttc gagcgtggcc atgccataac aagtgaccgc caccacgaag
      601 aacttgagca ccggatcctc gcgcacccgg tcccatgctc cacgcaacgt gagcaggccg
      661 ttgagcatgc cgccccagct cggtgcgatc agcatcacgc tgaacacaac gcccagactt
      721 tgcgcccatt cgggcagcgc gctgtagagc aggtggtgcg gtccggccca gatgtatatg
      781 aagatgagcg accagaagtg gatgatggag agcttgtagc tgtacaccgg gcggttggcg
      841 gccttcggca ggaaataata catcaacccg aggaaggggg tcgtgaggaa gaatgccacc
      901 gcgttgtgcc cgtaccacca ctgcaccagc gcgtcctgca ccccggcgta ccaactgtag
      961 ctcttgccca gcgttaccgg tatgctgatg ctgttcacga tgtgcagcat ggccaccgtg
     1021 acccacgtgg cgatgtagaa ccagatggcc acgtacaaat gccgctcgcg gcgcttgagg
     1081 atcgtgccca tcatgttgat cccgaacacg acccacacga tcgtgatggc gatgtcgatg
     1141 ggccattcga gttccgcgta ctccttcccc tgggtcatgc ccagcggcag ggtgatcgcg
     1201 gccgacagga tgatcagctg ccagccccag aagtggatcc tgctcagcac atcgctgtac
     1261 atgcgcgtct tcaacaggcg ctgcatgctg taataggcgc cggcgaagaa ggcgttgcct
     1321 acgaaggcga agatcaccgc gttcgtgtgc agtggccgga tacggccgaa actgatccac
     1381 ttcgcgaagt tcaggtccgg aatgaccagt tccagggccg cgatcagccc taccagcatg
     1441 cccaccgcgc cccagaagat ggtgatgaac aggaagtcct ttgcgatgcg gttgtcgtac
     1501 ctgaaacgct cggtcatggg gtcttggttt ggcgcgggtc gtcttcgtgc aggatgcgga
     1561 cggcggggga acggtcatcg tcgaactgcc cacggcgcac cgcatggatg aagccgagca
     1621 ggaacagtcc ggctaccgat gtgcttacca ggagaagaag gataatgaca ctcatcaggg
     1681 ttgcctgccg cggcgcgaaa gtgcccgggt cgggaaaggt aaatactgac gatcgtcagt
     1741 cgacgatact gacaggggtc aatgccggat ggtccggtcc ccggaagatg ggcctgcact
     1801 tgcccttcag cggagctccc tcgccccgat cgccgtggcg gccgtgacga agctcaccac
     1861 gctgatgctg ctcaggggca tcagaatggc ggcgatcaac ggcgtcatgt ggccggctac
     1921 ggccagggac accccgatga tattgtaacc caatgagatg caaagactcg cgatgacgat
     1981 acggcgcgca cggcgagcca tgcggaggaa atggggcagt ccggcaatgg ctggcccgtc
     2041 catgatcgca tcgctggcgg gggtgagcgc cgcgctcgtt tcggttacgg tgatgcccac
     2101 atcggcttgg gccagtgccc ccgcatcgtt caggccgtcg cccaccatca gtacgcaccg
     2161 tccccgcccc tgcgcttcgt ggatgccgcg ctgcttgtcc atcggcgaac accccgtgcg
     2221 tatgctgccc gcaccgaatg catccgatag accgggatcc acttgcgaat caccggtgag
     2281 cagtgccaac ggcatgcgtt c
//
LOCUS       DAOQOC010000440         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28932_length_2295_cov_2.870089, whole genome shotgun sequence.
ACCESSION   DAOQOC010000440 DAOQOC010000000
VERSION     DAOQOC010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28932_length_2295_cov_2.870089"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PL106_07810"
     CDS             <1..842
                     /locus_tag="PL106_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33013.1"
                     /translation="PGVVCNDGNPNTINDVYNANCQCVGTPTGSGVDLNVKAVLEGPY
                     VAGTGLMNDAMRAASLIPATEPYTALGLPQIGGGGETVAPSVLAVTGNNAVVDWVRVE
                     LRSSGAPATIIATRQGLLQRDGDITATDGTSALSFSGVSAGNYYVALRHRNHLGIMTS
                     AAIALGGGPTTVDFRNVATTTYGTNARKSITGTFPAQALWAGDATSNGQVKYTGSSND
                     RDPLLTTVGSTTPNATVNGYSTRDVNLNGQVKYTGSANDRDPILINVGSTTPNATRSA
                     QLP"
     gene            968..>2295
                     /locus_tag="PL106_07815"
     CDS             968..>2295
                     /locus_tag="PL106_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33014.1"
                     /translation="MRATCVIRYLFQVFFLFVGTATGQISVSEAASVQLSASVQASPA
                     RVTLSWTSFPGATGYTVHRKAHSTSSWGSAIGTTAGNVNTFQDNTVAVGTLYEYRVMR
                     TAPPGTGYGYVCSGIELPPVASMGRIVLLVDAAIAPGIGPQLTQLQSDLKADGWVVTR
                     HDVAPGTSVPAVRNLVIGTYNTDPANTKAVYIIGHVPVPYSGNIAPDGHTEQHMGAWP
                     CDAYYGEMNGTWTDNTVNNPSGWSWVRNIPGDGKFDQDSPPNAVELQVGRVDMNDLPA
                     FGQSQTQLLAAYLDKAHQFRTKGFTPQVRGIMRDMMEDLTTPMAGSGWMSMSALVTPG
                     NITEVGYSDPAWLEDLVNGQSYLWTYGSGGGLIENDNGTLMFNQAIKVMTTTGLASKA
                     WDGVFNMSYSSYTGDWNNRNNVLRAVIASGHALTTVYAGPPNWWFHPMGMG"
BASE COUNT          481 a          712 c          736 g          366 t
ORIGIN      
        1 tgccgggcgt tgtgtgcaac gacgggaatc cgaacacgat caacgacgtg tacaatgcca
       61 actgccagtg cgtgggcaca ccaaccgggt cgggggtgga tctgaacgtg aaagccgtgt
      121 tggaaggacc ctatgtcgct ggaacgggct tgatgaacga cgccatgcgg gcggcctcat
      181 tgatcccggc gaccgagccg tacacggcgc ttggcctgcc acagatcggt ggagggggtg
      241 aaacagtggc cccatccgtg ctggccgtca ccggcaacaa cgccgtggtg gattgggtgc
      301 gtgtggagct tcgttcatcc ggagcacccg ccacgatcat cgccacacgg caggggcttc
      361 tgcaacggga tggggacatc acggccaccg atggtacgag cgctttgtcc ttcagtggtg
      421 tgagcgccgg caactattac gtggccctgc ggcaccggaa ccatctgggc atcatgacct
      481 cggcggctat cgcactgggc ggtggtccga ccacggtgga cttccggaat gtggccacca
      541 ccacctacgg gacgaatgcc cggaagagca tcacgggaac cttccccgca caagcgcttt
      601 gggctggcga tgccacgtca aatggacaag tgaagtacac cggaagctcc aacgaccgcg
      661 atccgctgct gaccacggtg ggcagtacga cacccaatgc cacggtgaac gggtacagca
      721 cacgcgatgt caacttgaat ggccaggtga aatacaccgg cagtgccaat gaccgcgacc
      781 cgatcctgat caacgtgggc agcacgactc ccaacgccac gcgaagcgct cagcttccct
      841 gaggaaccgg gcggcacgat ccgcgggcgg gcatcccaac ggatgcccgt tcgcatgttg
      901 atagggcggg gatcggaaga tccggcacag ggtatcttcg acccggatcc ggaatctcca
      961 gaccgtcatg cgggcgacct gtgtcatccg ataccttttc caggtatttt tcctgttcgt
     1021 cggcaccgcg accggccaga tcagcgtgtc cgaggcggcc agcgtgcagt tgtccgcctc
     1081 ggtacaggca tcaccggcac gcgtcacgct cagctggacg tcgttccccg gggccacagg
     1141 atacaccgtg catcggaagg cccacagcac atcctcctgg ggatcggcca tcgggaccac
     1201 ggccgggaat gtgaacacct tccaggacaa cacggtggcc gtcggcacgc tgtatgaata
     1261 ccgggtcatg cgcacggccc cgcccggcac gggatacgga tacgtatgtt caggtataga
     1321 actaccaccg gtcgcctcca tgggccgcat cgtactgctg gtggacgcgg ccatcgcccc
     1381 gggcatcggt ccgcaactca cccagttgca gagcgacctg aaagcggacg gatgggtggt
     1441 gacgcgtcac gacgttgcac cgggcacgtc ggtaccggcg gtgcgcaacc tggtcatcgg
     1501 cacgtacaac accgatccgg cgaacacgaa ggccgtgtac atcatcgggc atgtgcccgt
     1561 accgtacagt ggcaatatcg caccggacgg gcatacggaa cagcacatgg gcgcctggcc
     1621 gtgcgacgcg tattacgggg agatgaacgg cacatggacc gacaacacgg tgaacaaccc
     1681 ctcgggctgg agctgggtac gcaacattcc cggcgatgga aaattcgacc aggacagtcc
     1741 tccgaacgcg gttgagctgc aggtgggcag ggtggacatg aatgaccttc ccgcattcgg
     1801 ccagagccag acgcagttgc tggcggccta cctggacaag gcgcaccagt tccgcaccaa
     1861 gggattcacc ccgcaggtgc ggggcatcat gcgcgacatg atggaggacc tgaccacgcc
     1921 catggccggc agcggatgga tgagcatgtc cgccctggtg acacccggaa acatcaccga
     1981 agtcgggtac agcgatccgg cctggctgga ggacctggtg aacggccaga gttacctgtg
     2041 gacctatggc agcggtggcg ggctgatcga gaacgacaac gggaccctga tgttcaacca
     2101 ggcgatcaag gtgatgacca ccacaggcct ggcctccaag gcgtgggacg gcgtgttcaa
     2161 catgtcgtac tccagctata cgggcgattg gaacaaccgc aacaatgtgc tgcgcgcggt
     2221 gatcgccagc ggacacgcac tcacaaccgt gtacgcaggg cccccgaact ggtggttcca
     2281 ccccatgggc atggg
//
LOCUS       DAOQOC010000441         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28953_length_2294_cov_4.299687, whole genome shotgun sequence.
ACCESSION   DAOQOC010000441 DAOQOC010000000
VERSION     DAOQOC010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28953_length_2294_cov_4.299687"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PL106_07820"
     CDS             complement(<1..1907)
                     /locus_tag="PL106_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33015.1"
                     /translation="MRRRLSTLTLAIALCMPSWLSAQTESEPNDNSAQANALTLGTAM
                     TGDIGGAPCASGTSNDWFVITTTQDGTLSLNTNTTTSGGPGALRVHVYDPNGNLINYY
                     DHGADGTPVNQTSTLACVGKGTYYFQLARLNSECYTYAFTVTLSTPVFADDTEDNDNT
                     GQATALAHNTYAQGHLNFGYTGDNDDWYAFTTTVEGSSTFTTIAESEGGGAVRTHLYD
                     GNGNLISYFDAGSGAVHDDDTTSFTYDCLGQGNYYLRLVSIGGCGHSYKLKFQSTGAV
                     YANDLEPNNSAGQAFTNGALAPNTFTEGHINFTYYGDNSDWYFIQTPAEGTIRLTAIA
                     ERVPVANGNIRVHLLDGNGNLITYWDPVAGGSNTAVTTVVEYDCAGQGDYYLQVVSIN
                     GCGISYRLKYETLPAVYGNDAEPNNSSGEAFSNGPLAHNTFTEGHINFTYYGDNSDWY
                     FIQTPAEGAMSITMIAERVPVANGNLRVHLHDGNGNLITYWDPLAGGGNTPTTSVLGY
                     DCLGQGNYYLQVVSINGCGISYKLKYQTIPAVFGNDAEPNNSFGQALVLNPDSSSASG
                     HINFTYYGDNDDFYRVVLPGAGNIAFDLEAENAAGGALRVHLKDGNGNLINYSDHTVG
                     ASHTPAGSAVA"
BASE COUNT          395 a          639 c          732 g          528 t
ORIGIN      
        1 aacgccaccg ctgaaccggc cggtgtgtgc gatgccccga ccgtatggtc cgagtaattg
       61 atcaggttcc cgttcccatc tttcaagtgt acccgcaacg cgcctcctgc cgcgttctcc
      121 gcttccaggt cgaaggcgat gttgcccgcg ccgggcagca ccacgcggta aaagtcatcg
      181 ttgtcgccat agtaggtgaa gttgatgtga ccggaggcgc tcgacgaatc agggttcagc
      241 acaagggcct gtccaaagct gttgttcggt tcggcatcgt tgccgaatac cgccgggatc
      301 gtttgatact tcagcttgta gctgatgcca catccgttga tgctcaccac ttgcaggtag
      361 tagttcccct gtccgaggca gtcatatcca agaacggagg tggtgggcgt gttgccaccg
      421 cccgcgagcg gatcccagta tgtgatcaga ttgccgttgc catcgtggag atgaacgcgt
      481 aggttcccgt tcgcgaccgg cacgcgctcg gcgatcatcg ttatactcat ggcgccttcg
      541 gccggggtct ggatgaagta ccagtcgctg ttgtcgccgt agtaggtgaa gttgatgtgc
      601 ccttcggtga aggtgttgtg ggcgagtgga ccattggaga atgcctcgcc tgagctgttg
      661 ttgggttcgg catcgttccc gtacaccgct ggtagggtct catacttcaa gcggtagctg
      721 atcccgcaac cattgatgct caccacctgc aggtaataat cgccctggcc tgcgcagtcg
      781 tattccacaa cggtcgttac agccgtattg ctaccgcccg ccacgggatc ccaataggtg
      841 atcaggttcc cgttgccgtc gagcagatgc acccggatgt tgccgttcgc aacgggtacg
      901 cgctctgcga tcgcggtgag gcgtatggtg ccttcggccg gggtctggat gaagtaccag
      961 tcgctgttgt cgccgtagta ggtgaagttg atgtgccctt cagtgaaggt gttgggggcg
     1021 agagcaccat tggtgaatgc ctggccggcg ctgttgttcg gttccaggtc gttggcgtag
     1081 acggctccgg tggactggaa cttcaacttg tagctatggc cgcatccgcc gatgctcacc
     1141 aggcgcaggt agtagttgcc ctggcccaag cagtcgtatg tgaaactggt ggtgtcatca
     1201 tcgtgaacag ctcctgaacc agcatcgaag tagctgatca ggtttccgtt gccatcatag
     1261 aggtgggtgc gcacagcgcc ccctccttcg ctctcggcga tcgtggtgaa ggtggaggaa
     1321 ccctcgacag tggtggtgaa ggcgtaccag tcgtcgttgt cgcctgtgta gccgaaattc
     1381 agatggcctt gcgcgtaggt gttgtgcgcc aaggccgtgg cctggccggt gttgtcgttg
     1441 tcctccgtgt catcggcgaa taccggagtc gaaagggtga ccgtgaacgc gtaggtgtaa
     1501 cattcgctgt ttagccgtgc cagttggaag tagtacgttc ccttgcccac gcatgcgagc
     1561 gtggaggtct ggttcacggg cgtaccgtca gcgccatggt cgtagtagtt gatcagattg
     1621 ccattgggat cgtacacatg cacacgcagc gcacccggac caccggaggt ggtggtgttg
     1681 gtgttgaggc tgagggtgcc atcctgcgtg gtggtgatca cgaaccagtc gttgctggtg
     1741 ccgctggcgc atggcgcacc accgatatcg ccggtcatgg ccgttcccaa ggtgagggca
     1801 ttggcttgtg cggagttatc gttgggttcg ctctccgttt gtgcggaaag ccaactgggc
     1861 atgcacaggg cgatcgcgag cgtcagggtg gaaagtctcc ttcgcatagg tctgggttta
     1921 ggtccgtcaa aactccccca cggtcgacaa cgggtcaatc ccgaattctc gtgaggtcga
     1981 tccatgcgcg gccccaggtg gcacccatca gcaggcctga tcctccgtgc cagtgcgttt
     2041 ctgctcaggc tgcgagcggt gttctcaacg gatgtgcatg atcgcccggc caggctgcta
     2101 tcaataagcg agggggccgt tccttgcgga acggccccct gctcaaaaag ctcctgcgct
     2161 tactggatca ccaggcgctg ggtgtaacgc ttgccatcga tggtcacgtg taccatgtag
     2221 agccctgcgc ccgtatcgcg gcccaggtcc atgcggtggt tcacgatgcc ttcggcggcc
     2281 acggtgcgct cagc
//
LOCUS       DAOQOC010000442         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_28998_length_2293_cov_2.281055, whole genome shotgun sequence.
ACCESSION   DAOQOC010000442 DAOQOC010000000
VERSION     DAOQOC010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28998_length_2293_cov_2.281055"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1332
                     /locus_tag="PL106_07825"
     CDS             109..1332
                     /locus_tag="PL106_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNA33016.1"
                     /translation="MKALFANHLKGDRTIWMVALVLGIVSILAVYSACSWMAWRNDGG
                     TLRVLGKHSVMLAAGGFIMYVAANTRYTLFSRLARLALPVTIALLALTLIIGSNVNGA
                     SRWLVIPGLGVTFQTSDLARVTILLYLARVLGRPYEEPWTFKETALRVGLPVAAVCGL
                     ILPANFSTAALLGLTCLLVLFVGQVPLKHIGSLAGLGVLAFVLLLLLAKTWPDLLPRV
                     ATWEKRIENWGAKDDGVLTPEERDANYQTNNAKIAIVSGGLLPNGPGTGTSRNFMPHP
                     ESDMIYAFIIEEYGSILGGLGLLLLYLILMFRSMKVSDHAEKDFGKLAAIGLALSLVL
                     QALVNMAVAVNLLPVTGQPLPLVSMGGTSMWFTCLSIGIILSVSRGLTDVAGPAPKSR
                     PRTAHAIPTDAAAAA"
     gene            1337..>2293
                     /gene="murG"
                     /locus_tag="PL106_07830"
     CDS             1337..>2293
                     /gene="murG"
                     /locus_tag="PL106_07830"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNA33017.1"
                     /translation="MIAGGGTGGHIFPAVAIANAVKEREPATEFLFVGAEGKMEMDKI
                     PAAGYRIEGLPIRGFQRSAIWKNIGLPWRLFKSMLKARELVRTFKPDVAVGVGGYASG
                     PVLEAAQRLKVRTLIQEQNSFPGRTNIWLSRRADRICVAYGGMEKFFPAEKTLLTGNP
                     VRQEMVRLTGKRPRGLEEFGLRDGAPTVFVTGGSLGARGINLGIEAALPALKENGVQV
                     IWQTGSPFYRQALEAVAGIGYTDCKVHEFVARMDHAYAVADLVVARAGAISVSELQLV
                     QLPAILVPLPTAAEDHQTMNARALTDLGAAVLVKDADAVRTLG"
BASE COUNT          403 a          764 c          713 g          413 t
ORIGIN      
        1 ctggaattcc tgcacgcggt atccgacctg tgatgcgaac gatacggccc ggccacgata
       61 ccctccgccc gggccttgcc gcgaccccat acccgaaaca agtgattcat gaaagccctg
      121 ttcgccaacc acctcaaagg tgaccgcacc atctggatgg tcgcgctggt actcggcatc
      181 gtcagtatcc tggccgttta cagcgcatgc agctggatgg cctggcgcaa cgacggcggg
      241 accctccgcg tgctggggaa acacagcgtc atgctcgcgg ccggcgggtt catcatgtac
      301 gtggccgcca acacgcgtta caccctgttc tcgcgcctgg cacgcctggc actccctgtg
      361 accatcgcac ttctcgccct caccttgatc atcggttcca acgtgaacgg cgcgtcgcgc
      421 tggctcgtca tccccggcct cggtgtcact ttccagacca gtgatctggc ccgcgtgacc
      481 attctcctgt acctcgcccg tgtgctcggc cgcccgtacg aggaaccctg gacattcaag
      541 gagaccgccc tgcgtgtagg cctgcccgtg gccgccgtct gcggcctgat cctgccagcc
      601 aacttctcca cggccgcact cctgggcctc acgtgcctcc tggtcctgtt cgtggggcag
      661 gtacccctga agcacatcgg ctcgctggcc gggctgggcg tgctcgcttt cgtgctcctg
      721 ctcctgctgg caaagacatg gcccgacctc ctcccgcgcg tggccacttg ggaaaaacgt
      781 atcgagaact ggggagcgaa ggacgacggg gtgctgacac ccgaggaacg cgacgccaac
      841 taccagacga acaacgccaa gatcgccatc gtgagcggcg ggctcctgcc caacggcccg
      901 ggtaccggca cctcgcgcaa cttcatgccg catccggaga gcgatatgat ctacgccttc
      961 atcatcgagg agtacggctc gatcctcggg ggactgggcc tgctcctgtt gtacctcatc
     1021 ctgatgttcc gctccatgaa ggtctctgac cacgccgaga aggacttcgg caaactcgcc
     1081 gcgatcgggc tcgcactcag ccttgtgctc caggcgttgg tgaacatggc cgtggccgtg
     1141 aacctgttgc ccgtgaccgg ccaaccgttg ccactggtga gcatgggcgg caccagcatg
     1201 tggttcacct gcctgtccat cgggatcatt ctgagcgtga gccggggcct gaccgatgtt
     1261 gcgggacccg ccccgaagtc ccgtccgcgc accgctcacg ccatacctac ggatgcagcc
     1321 gccgccgctt aggatcatga tcgccggtgg tggtaccggt gggcacatct tccccgccgt
     1381 ggccatcgcc aatgcggtga aggaacgcga gccggcgacc gaattcctct tcgtgggcgc
     1441 cgaagggaaa atggaaatgg acaagatccc agcggccggg tatcgtattg agggtctgcc
     1501 gatccgtggc ttccaacgct cggcgatctg gaagaacatc gggctgccgt ggcgcctgtt
     1561 caagagcatg ttgaaggcac gggaactggt gcgcacattc aagccggacg tggccgtggg
     1621 ggtgggcggc tacgccagcg gtcccgtgct cgaagcggca caacggctca aggtgcgcac
     1681 cctgatccag gaacagaaca gcttcccggg ccggaccaat atctggctgt ccaggcgggc
     1741 ggaccgcatc tgtgtggcct atgggggcat ggaaaagttc ttcccggccg aaaagaccct
     1801 cctcacgggc aaccccgtga ggcaggagat ggtgcgactc accggaaaac gtcctcgcgg
     1861 gctggaagaa ttcggactgc gggatggtgc gccgaccgtg ttcgtcaccg gtggaagtct
     1921 cggtgctcgt ggcatcaacc tcggcatcga agccgccctc cccgcgctga aggagaacgg
     1981 cgtgcaggtg atctggcaga ccggttcacc attttaccgg caagctttgg aggccgtggc
     2041 cgggatcgga tacacggatt gcaaggtgca tgaattcgtc gcgcggatgg accacgccta
     2101 cgccgtggcc gacctggtgg tggcacgtgc cggggcgatc agcgtgagcg aactgcaact
     2161 ggtacaatta cccgcgatcc tggtgcccct gcccaccgcc gccgaggacc accagaccat
     2221 gaatgcccgc gccctcacgg accttggtgc cgccgtgctg gtcaaggatg ccgatgccgt
     2281 ccggaccctg ggt
//
LOCUS       DAOQOC010000443         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29010_length_2292_cov_4.003129, whole genome shotgun sequence.
ACCESSION   DAOQOC010000443 DAOQOC010000000
VERSION     DAOQOC010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29010_length_2292_cov_4.003129"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1612)
                     /locus_tag="PL106_07835"
     CDS             complement(53..1612)
                     /locus_tag="PL106_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNA33018.1"
                     /translation="MPVPDTFLKQMTDGYTFSGGSLLLGAPLLNGECAATAPVRVPLR
                     TMNRHGLIAGATGTGKTKTLQTIAEQLSLNGVPSLLMDIKGDLSGIAAPGTANEKIIA
                     RHDKLGFPFEPVALPVELLTLSDEPGARLRATVSEFGPVLLSRILDLNETQQSVLALV
                     FKYCDDMGMALLDIKDLRRVLQFTTQEGKAELQKAYGSVSPATVNIIMRKLIELEQQG
                     AEKFFGERSFDVNDLLAQRDGKGVVHIVRLTDLQSRPQLFSTFMLCLLAEVYHTFPEV
                     GDPEKPKLCLFIDEAHLIFNTATPALLQQIETIIKLIRSKGVGVYFCTQNPMDVPDSV
                     LGQLGLKVQHALRAFTAKDRTAIKKTAENYPLTDFYKTEELLTSLGIGEALVTALSEK
                     GTPTPLAHVLMRAPGTRMDVLSPLEIQGLVATSAIAKKYNEVIDRESAFEILEKRMKG
                     EDGNDADEPQEEKRPKPGKEEKSTLEKLSENTMVRQVGRTVANTLTRSLLGVLGLRTT
                     TRRRRSGGLFG"
     gene            complement(1686..1889)
                     /locus_tag="PL106_07840"
     CDS             complement(1686..1889)
                     /locus_tag="PL106_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNA33019.1"
                     /translation="MPILKVIEILASSKNSWEEAANNAVKEASKTVRGIRSVNVQNQS
                     AVVEKGKIVEYRVNCKISFSVEK"
     gene            complement(1944..>2292)
                     /locus_tag="PL106_07845"
     CDS             complement(1944..>2292)
                     /locus_tag="PL106_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33020.1"
                     /translation="ITAAADSVKQVIGGGDGFGLDDMLNLFSSAQNNSNADGILSNIS
                     QVFNGKLTSDVGLSAQQAGGVKDLLLPMIMNLISGKVGGNANNLQGLLSAFTGQGGGL
                     AEMAGGLLKGLFK"
BASE COUNT          382 a          702 c          695 g          513 t
ORIGIN      
        1 ccacaggccc acaacagcgt ggccaggaca agggaaaggg attgccccat gatcaaccga
       61 acaagcctcc gctcctgcgc ctccgggtgg tcgtcctcag cccaagtacg ccgagcaaac
      121 tacgtgtcag cgtgttggcc accgtgcgcc cgacctgacg gaccatggtg ttctcgctga
      181 gcttctccag ggtgcttttc tcctccttcc cgggcttcgg ccttttctct tcttgcggtt
      241 catccgcatc gttgccgtct tcgcccttca tccgcttttc caggatctcg aacgcactct
      301 cgcgatcgat cacctcgttg tacttcttcg ctatcgcgct tgtggccacc agaccttgga
      361 tctccagcgg tgaaagcacg tccatgcggg taccgggcgc gcgcatcagc acatgcgcca
      421 agggtgtcgg ggtgcctttt tcactcagcg cggtcaccag ggcctcgccg atgcccaggg
      481 aggtgagcag ttcctcggtc ttgtaaaaat cggtgagcgg gtagttctcg gcggtcttct
      541 tgatggcggt gcggtccttg gcggtgaagg cgcgcagggc gtgctgcacc ttcaggccga
      601 gctggcccag cacgctgtcg ggcacgtcca tcgggttctg cgtgcagaag tacacgccca
      661 cgcccttgga acggatcaat ttgatgatgg tctcgatctg ctgcaacagc gcaggggtgg
      721 ccgtgttgaa gatcaggtgg gcctcgtcga tgaacaggca gagcttgggt ttttccgggt
      781 cccctacctc cgggaatgta tgatagacct cggccagcag gcagagcatg aaggtgctga
      841 agagctgcgg ccggctctgc aggtcggtga ggcgcacgat gtggaccacg cccttgccat
      901 cgcgctgggc gagcaggtca ttcacatcga aactgcgctc accgaagaac ttctcggccc
      961 cctgctgttc cagttcgatc agtttgcgca tgatgatgtt caccgtggcg gggctcaccg
     1021 agccgtacgc cttctggagt tcggccttgc cttcctgggt ggtgaactgg agcacgcgcc
     1081 gcagatcctt gatatcgagc agggccattc ccatgtcatc gcagtacttg aacaccaggg
     1141 ccagcacgct ctgctgggtc tcgttcaggt ccaggatccg gctcaggagc acggggccga
     1201 attcgctcac cgtggcccgc aggcgtgccc ccggctcgtc gctcagggtg agcagttcca
     1261 ccggcagggc taccggctcg aacgggaagc ccaatttatc atggcgtgcg atgatctttt
     1321 cattggccgt gccgggtgcg gcgatcccgc tcaggtcgcc tttgatatcc atcagcagcg
     1381 atggcacgcc gttcaagctg agctgctcgg cgatcgtttg cagcgtcttg gtcttgcccg
     1441 tgccggtggc cccggctatg aggccgtggc gattcatggt gcgcaggggc acgcgcacgg
     1501 gggccgttgc cgcgcattcg ccgttcagca agggcgcccc gagcagcaga ctgccaccac
     1561 tgaaggtgta gccatcggtc atctgtttca ggaaggtgtc gggaacgggc atggcggtag
     1621 gatctatgct gcgaaacaaa cgagaaaagg ggcctgcgcc ccttcccctt gtgtagttgt
     1681 gccggttact tctccacgga gaagctgatc ttgcaattca cgcggtactc cacgatcttg
     1741 cctttctcca ccacggcact ctggttctgc acattcacac tgcggatgcc gcgcacggtc
     1801 ttgctggcct ctttcaccgc gttgttcgcg gcctcctccc agctgttctt gctgctggcg
     1861 aggatctcga tcactttcag gatgggcatg ggttctgagg tttggttggt tgctcgaatg
     1921 gtagctgcgc gcgatcgctg aggttacttg aacagacctt tcagcaagcc accagccatt
     1981 tccgcgagcc caccaccttg accggtgaag gcgctgagca gaccttgcaa attgttggcg
     2041 ttgccgccca ccttgccact gatcaggttc atgatcatgg gcaggaggag gtccttcacg
     2101 ccaccggcct gttgtgcgct caggccgaca tcactggtca atttaccgtt gaacacctgg
     2161 ctgatgttgc tcaggatacc atcggcgttg ctgttattct gggcggaact gaagaggttg
     2221 agcatgtcgt ccagaccgaa tccgtcgcca ccgccgatca cttgcttcac gctgtcagcg
     2281 gcggcggtaa tg
//
LOCUS       DAOQOC010000444         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29012_length_2292_cov_3.476084, whole genome shotgun sequence.
ACCESSION   DAOQOC010000444 DAOQOC010000000
VERSION     DAOQOC010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29012_length_2292_cov_3.476084"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..600
                     /locus_tag="PL106_07850"
     CDS             211..600
                     /locus_tag="PL106_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33021.1"
                     /translation="MARAHQAGITVVPLTGPSSLLLALAASGLNGQRFTFHGYLPKGT
                     NERRQALRTIEQEAHRTGSAQLAIETPYRNNAFLADTLATCSDRTRLCIAVDIGQPGG
                     SVITRSIAAWKKAAPDLNDRPCVFIIG"
     gene            complement(590..1498)
                     /locus_tag="PL106_07855"
     CDS             complement(590..1498)
                     /locus_tag="PL106_07855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNA33022.1"
                     /translation="MTRRILILLLPLLLIACGRGVFPGFKPLDADVHYKLRSLGDGDR
                     PPADSDSVLVRVRMALHGEAPGSLFSTEHWFGHVGDVLPIGAGVIRFQEGDSMSFIAR
                     ASRLDWDAIGAERFSVPDTAWIDLEVSLRDTRSNEESRALAEERRWARTEAMEDSALA
                     RFFRASPEKWQYSMGVHYVLEPRDPKRPQAQSGQMLTIGYTARSLEDGRVIDDTYASG
                     QHLTFRLGDPDQVIAGIGIAVRLLPEGGKGRFVIPSRLAFGADGSTGRIVPPYTPLLY
                     ELELRDVTGRAADTLSVDSAAVGINR"
     gene            complement(1513..1782)
                     /locus_tag="PL106_07860"
     CDS             complement(1513..1782)
                     /locus_tag="PL106_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNA33023.1"
                     /translation="MKRLVFEQGAFDDFCDWGIYDRPRFIKIHELLKAILRDPFKGLG
                     KPEALKGDLKGCWSRRIDDTHRLVYRIARDGSVEVISCKGHYGDR"
     gene            complement(1779..2036)
                     /locus_tag="PL106_07865"
     CDS             complement(1779..2036)
                     /locus_tag="PL106_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33024.1"
                     /translation="METSVRIKISELDMDLLERIKRLFGRDREVTLTIRSATDFGLTK
                     RETKKAYLARLERAIKNLEEGRRVELTEEELDGIVLQRLKG"
BASE COUNT          451 a          743 c          638 g          460 t
ORIGIN      
        1 aagcatagcg caaaacagga atgtttatac tattcaaaga tcatcatttt tattaatgat
       61 aatgggaata atcttgagtt tttatgaaac ggcggggggc atccgccagt gaatggaagg
      121 tcagaccgac tttcggcgcc atgactggtt tgcactctgg gcaaaatgct tcatcctaaa
      181 aaccgtctta tccagaaaaa gcacaacaga gtggcccgtg cccatcaggc gggcatcacg
      241 gtcgttccgc tcaccgggcc ttcctccctc ctgctcgcac tcgcggcatc cggcctgaac
      301 gggcagcgct tcaccttcca tggatacctt ccaaaaggga ccaatgaacg gcggcaggcc
      361 ctgcgcacca tcgaacagga ggcccaccgt accggcagcg cgcaactcgc gatcgaaacg
      421 ccctatcgca ataacgcttt cctcgccgac acgctcgcca cctgctcgga ccggacgcgc
      481 ctgtgcatcg ccgtggacat cggccagccg gggggctccg tgatcacccg atccatcgcc
      541 gcctggaaga aggccgcacc cgacctgaac gatcggccct gcgtgttcat catcggttga
      601 tccctacggc tgcgctgtcc acggataagg tgtccgcggc ccggcccgtc acgtcccgca
      661 gttccagttc gtacaacagc ggggtgtacg gcggtacgat ccgtccggtg gaaccatcag
      721 caccgaaggc cagccttgaa ggaatgacga aacgcccctt cccgccttct ggcagcaggc
      781 gtaccgcgat cccgatgccg gcgatcacct ggtcggggtc gcccaggcgg aaggtgaggt
      841 gctggcctga agcataggta tcgtcgatca cgcggccatc ctccagggag cgggccgtgt
      901 atccgatggt gagcatctgt ccactttgtg cttgcggtcg cttcggatct cgtggttcca
      961 gtacataatg aacacccatg gaatactgcc acttctcagg cgatgcgcga aagaaccgcg
     1021 ccaacgcgct gtcctccatg gcttcggtgc gggcccagcg gcgttcctcg gccaaggcgc
     1081 ggctctcttc gttggatcgt gtatcgcgga gtgacacctc caggtcgatc cacgccgtgt
     1141 ccggtacgct gaaacgttcc gccccgatcg cgtcccaatc cagccgtgat gcacgcgcta
     1201 tgaaactcat gctgtctccc tcttggaagc ggatgacacc cgcgccgatc ggcaatacat
     1261 cacccacgtg cccgaaccag tgttcggtac tgaagagcga tccaggggcc tcaccgtgca
     1321 gggccatgcg cacccgcacc agcacgctat cgctatcagc aggtggacga tcgccatcgc
     1381 cgagcgagcg cagcttgtag tggacatcgg cgtcaagcgg cttgaagccg gggaagaccc
     1441 cacggccgca ggcgatgagc agcagcggca aaagcaggat gaggatccgg cgggtcatcg
     1501 gtcgcaacaa ggctacctgt cgccgtaatg gcccttgcaa ctgatcactt ccaccgaacc
     1561 gtccctggcg atgcggtaca cgagtcggtg cgtatcgtcg atgcgtcgtg accagcatcc
     1621 cttcaggtcc cccttgaggg cttcgggttt gccgagccct ttgaacggat cgcgcaggat
     1681 cgccttcagt agttcatgga tcttgatgaa gcggggcctg tcatatatac cccagtcgca
     1741 gaagtcgtcg aaggcgccct gctcaaaaac aaggcgtttc atcccttcag ccgttgcagc
     1801 acgatgccgt ccagctcttc ctcggtgagt tccacacgcc tgccctcctc caggttcttg
     1861 atggcgcgtt ccaagcgggc caaataggcc ttcttcgttt cccgtttcgt gagtccgaag
     1921 tccgtggccg accggatggt gagcgttacc tcgcgatccc tgccgaagag ccgcttgatc
     1981 cgctccagca ggtccatgtc caattccgag atctttatcc gcacgcttgt ttccatgatc
     2041 gctttcccac tggtgggcca gcgaagatac ggacaaggtc cctggcccgt tccgcggacc
     2101 acaggtacct tcgcgcgctg atcaccaccc atggaattca tccagttcct catcgacctg
     2161 ctcaaggaca ccaaacccac gatcgaccag ttcatcgccg atcacggcaa gctcgtgtac
     2221 gggctgctct tcctcatcat cttcgtggaa acggggctgg tggtgatgcc atttcttccg
     2281 ggtgactccc tg
//
LOCUS       DAOQOC010000445         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29043_length_2291_cov_2.979428, whole genome shotgun sequence.
ACCESSION   DAOQOC010000445 DAOQOC010000000
VERSION     DAOQOC010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29043_length_2291_cov_2.979428"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PL106_07870"
     CDS             <1..821
                     /locus_tag="PL106_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA33025.1"
                     /translation="AFFAIGSFAAMGLFAIALCALNLLGVVFALPESLGGRDHARRIN
                     FKPIASTFASLRDLRFRDIFIIGFVYITAFSMMNVSIAFLWQQRYHLDVDQVGFLFSI
                     VGVLSAISQGALVGLFQRWWGERKMMVRGPILVGTGLVLIPFVPLGLRASMPMGPDGQ
                     PLHQALDQLFVLYSMVPMILISVGNACLNPSLISILSRQADPHEQGEVLGQNMGFGSL
                     GRVLGPMLSGLLYPLGKGLPFVVGGTIMLGALTLVFRYLRTGYKALDPASRPAD"
     gene            846..1715
                     /gene="sucD"
                     /locus_tag="PL106_07875"
     CDS             846..1715
                     /gene="sucD"
                     /locus_tag="PL106_07875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNA33026.1"
                     /translation="MSVLVNKNSKVIVQGFTGSEGTFHAEQMIEYGTRVVGGVTPGKG
                     GQSHLDRPVFETVNDAVVATGADVSIIFVPPPFAADAIMEAADAGIKVIVCITEGIPV
                     KDMVAAREYIRGKDCVLIGPNCPGVITADECKVGIMPGFVFKKGGVGIVSKSGTLTYE
                     AADQVVKAGLGITTAIGIGGDPIIGTTTLEAIRLFANDPETKAIVMIGEIGGDLEIRA
                     AKWIKENVKKPVIGFIAGETAPKGRTMGHAGAIVSGADESAAAKKKVMRECGIHVVDS
                     PATIGAKVKEVLG"
     gene            complement(1779..>2291)
                     /locus_tag="PL106_07880"
     CDS             complement(1779..>2291)
                     /locus_tag="PL106_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33027.1"
                     /translation="MRTALPLVVLMAGLAACCGKKHAAGQKDTTTEGQVATSSPRVLV
                     YRTKEDHADHVPVLLSADHSRIISYPHPSDLKTANGLPVPTPVGQGYLLDNRGISKDV
                     AYLGMSWAEYAALPEAPSMEKLNALIIDRDPLTELWEGDHRANLKDPVKEVSAWVEAG
                     ELDKHCKRLK"
BASE COUNT          400 a          702 c          737 g          452 t
ORIGIN      
        1 tcgccttctt cgccatcggc tccttcgcgg ccatgggcct cttcgccatc gccttgtgcg
       61 cactgaacct cctgggcgtg gtcttcgccc tgcccgaatc gctgggtggc cgggatcatg
      121 cccgacggat caatttcaaa cccatcgcca gcaccttcgc atcgctgcgc gacctgcgct
      181 tccgcgacat cttcatcatc gggttcgtgt acatcacggc cttcagcatg atgaatgtga
      241 gcatcgcttt cctgtggcag cagcgttatc acctggatgt ggaccaggtg ggctttctct
      301 tttccatagt cggggtgctc tcggccatct cgcaaggcgc actggtcggc ctgttccagc
      361 gctggtgggg cgagcgcaag atgatggttc gaggtcccat cctggtgggc acgggcctcg
      421 tgctgatccc cttcgtgccg ttgggcctgc gcgccagcat gcccatggga cccgatggac
      481 aacccttgca tcaggcgctg gaccagctct ttgtgttgta cagcatggta cccatgatcc
      541 tgatctccgt gggcaatgcc tgcctgaatc ccagcctgat ctcgatcctc agtcggcagg
      601 ccgatccaca tgagcagggt gaagtgctgg ggcagaacat gggcttcggt tcgctgggcc
      661 gggtgctggg gccgatgctc tcgggcctgc tctatccgct cggcaaaggc ctgccgttcg
      721 tggtgggcgg cactatcatg ctgggggctt tgacgctggt gttccggtac ctgcgcacgg
      781 ggtacaaggc gctggatccc gcttcccgcc ccgccgatta gtttcgccgc cgatcacatc
      841 acaccatgag cgtactcgtc aacaagaaca gcaaggtcat tgtacagggt ttcacaggca
      901 gtgagggcac ctttcacgct gaacagatga tcgagtacgg gacccgggtg gtcggtggcg
      961 ttacacccgg caagggtgga cagtcgcatc tggatcgccc cgttttcgag accgtcaatg
     1021 atgccgttgt ggccaccggc gccgacgtga gcatcatctt cgttccgccg ccattcgccg
     1081 ctgacgccat catggaagcc gccgatgcgg gcatcaaggt gatcgtgtgc atcaccgagg
     1141 gcatccccgt gaaggacatg gtggccgcgc gcgagtacat ccgggggaag gactgcgtgc
     1201 tcatcggccc caattgcccg ggcgtgatca ccgctgatga atgcaaggtc ggcatcatgc
     1261 cgggcttcgt gttcaagaag ggcggggttg gcatcgtgtc gaagtccggt acgctcacct
     1321 acgaggcggc cgatcaagtg gtgaaggccg gcctcggcat cactacggcc atcgggatcg
     1381 gtggcgaccc gatcatcggg accaccacgc tggaggccat ccggttgttc gccaacgacc
     1441 ccgagaccaa ggccatcgtg atgatcggcg agatcggggg ggacctggag atccgcgccg
     1501 ccaagtggat caaggagaac gtgaagaaac ccgtgatcgg cttcatcgcc ggggagaccg
     1561 cgccgaaggg ccggaccatg gggcacgcgg gcgccattgt gagcggagcc gatgagagcg
     1621 ccgccgccaa gaagaaggtg atgcgcgaat gcggcatcca cgtggtggac agcccggcca
     1681 ccatcggtgc caaggtgaag gaagtgctgg gctgagacca tcaacgacaa cgggcacccg
     1741 ttgccggatg cccgttcgtg ccggttgacg aaagggggtc acttcaaccg tttgcagtgc
     1801 ttgtccaact cacccgcctc cacccaggca gatacctcct tcaccggatc tttcaggttc
     1861 gcacggtggt cgccctccca tagctcggtc agcgggtcgc ggtcgatgat cagggcattc
     1921 agcttctcca tggacggggc ttcgggcaat gcggcatact cggcccagga catgcccaag
     1981 taggccacat ccttgctgat gccccggttg tccagcagat agccctgacc cacgggcgtt
     2041 ggcacgggca ggccgttcgc cgtcttcagg tccgaggggt gcgggtatga gatgatgcgt
     2101 gaatgatccg cagaaagaag tacgggcaca tggtccgcat ggtcttcctt ggtccggtac
     2161 acgagcacgc gtggcgatga ggtggccacc tggccttccg tggtggtgtc cttttgaccg
     2221 gctgcatgct ttttgccgca acacgcggcc aggccggcca tgagcacaac aaggggaagg
     2281 gcggtcctca t
//
LOCUS       DAOQOC010000446         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29144_length_2287_cov_3.541219, whole genome shotgun sequence.
ACCESSION   DAOQOC010000446 DAOQOC010000000
VERSION     DAOQOC010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29144_length_2287_cov_3.541219"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PL106_07885"
     CDS             complement(<1..1209)
                     /locus_tag="PL106_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNA33028.1"
                     /translation="MDLEASKNEDFNKLKLSDLNARLKKIHLGGGEKRIADHRAKGKM
                     TARERIDALLDPKSPRIEIGAFAADGMYKEHGGCPSAGTVVVIGYVSKRQCIVVANDA
                     TVKAGAWFPMTGKKNLRAQEIAIENRLPIIYLVDSAGVYLPMQDEIFPDKEHFGRIFR
                     NNAVMSSMGITQIAAVMGSCVAGGAYLPIMSDEALIVEKTGSIFLAGSYLVKAAIGED
                     IDNETLGGATTHSEISGVTDYKCKDDADCLKKIRAIMDKLGKPKDAGFSREKPAAPKS
                     DPKELHGIIPIERAKPYDMREVIARLVDDSDFTEYKEGFGQSILTAYARIDGWAVGIV
                     ANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQKLIPLVFLQDVTGFMVGTRSE
                     HGGIIKDGAKM"
     gene            1578..>2287
                     /locus_tag="PL106_07890"
     CDS             1578..>2287
                     /locus_tag="PL106_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33029.1"
                     /translation="MNVSCARSPLGLFLAIPMLLCACSNKELVQYKARLDRGSCSQWN
                     SGHTAIPVSAYDPEAVPAEVASDFSPQSLRIAHAIGVLPLLADHIHRTQVPSAERNLQ
                     QRVDLLEQRSLIAHHVDMASLEISAIASELDCEEERISQLAGYLKGLEDKKESQLTVA
                     AIAVGALGTLVTGVHLADEGDSNIDGAGIGFGIAGALLGTAMLFTEKRTDFRHPRNAI
                     GVIHAGTDPQGIFPPSVW"
BASE COUNT          362 a          712 c          727 g          486 t
ORIGIN      
        1 cattttagcg ccgtctttaa tgatgccgcc atgctccgag cgcgtgccca ccatgaatcc
       61 ggtgacgtct tgcaagaaga ccaaagggat caatttctgg ttgcagttca tgatgaagcg
      121 ggcagccttg tcggcgctgt cgctgtagat cacgccgccg aactgcattt cgcctttctt
      181 gctcttcacc accttccgtt gattggctac gatgcccacg gcccaaccat cgatgcgggc
      241 gtaggcggtg aggatgctct ggccaaaacc ttccttgtac tcggtaaagt cgctgtcgtc
      301 caccaggcgc gcgatcacct cgcgcatgtc gtagggtttg gcgcgctcga tggggatgat
      361 gccgtggagt tccttcggat cgctcttggg cgcggccggt ttctcgcggc tgaagcccgc
      421 atccttgggc ttgcccagct tgtccatgat ggcccggatc ttcttgaggc aatccgcatc
      481 gtccttgcac ttgtaatcgg tgacgccgct gatctcgctg tgggtggtgg cgccaccgag
      541 ggtctcgttg tcgatgtcct cgccgatggc ggcctttacc aggtagctgc cagccaggaa
      601 aatgctaccg gtcttctcca cgatcagggc ttcgtcgctc atgatgggca ggtaggcgcc
      661 gccggccacg cagctgccca tcacggcagc gatctgcgtg atgcccatgg aactcataac
      721 ggcgttgttg cggaagatcc tgccgaaatg ctccttgtcg gggaagatct cgtcctgcat
      781 gggcaggtac acgccggcgc tgtccaccag gtagatgatg ggcaggcggt tctcgatggc
      841 gatctcctgc gcgcgcaggt tcttcttgcc cgtcatgggg aaccaggcac cggccttcac
      901 ggtggcgtcg ttggccacca cgatgcactg gcgcttgctc acgtagccga tcaccaccac
      961 tgtgcccgcg ctggggcagc cgccgtgctc cttgtacatg ccatcggctg cgaaggcgcc
     1021 gatctcgatg cgcgggctct tggggtcgag cagggcgtcg atgcgctcgc gggcggtcat
     1081 cttgcccttg gccctatgat cggcgatgcg tttctcgccg ccgccaaggt ggatcttctt
     1141 caggcgggcg ttgaggtcgg agagcttgag tttgttgaag tcctcgttct tggaggcttc
     1201 caggtcgatc ttctcgggca tggttcgatg ttgggccgat gagcggatca gctgatggcg
     1261 tggtgcgaag atcgggcgca cggttcaatt ggctgggccg ctgagcgata tcggggcgtg
     1321 ccggtgtgat cccttcgctt cgcaaagcct ttgctcggga gcacaccgtc gggtcctacg
     1381 ctgtagccgg acttgtcagg gccagcggtt gcggcgctgc gggggcttcc ttcggtcgcc
     1441 tcctcgccgc acgcacccgc aaggccctgc ctgcgccggg ctgccgcttc ggcccctcac
     1501 gcggaaccga ggtaagcggg tgcccagcgg atcgaacggg tgtgctcgga tggtggtcca
     1561 gttgcgatct tgggaccatg aatgtatcct gcgcccgatc cccgctcggt cttttcctcg
     1621 ccattccgat gctcctgtgt gcctgctcga acaaggaact ggtccagtac aaagcacgcc
     1681 tcgaccgtgg ttcctgcagc caatggaaca gcggtcatac agcaataccg gtatctgctt
     1741 acgatccgga ggcggtgcct gccgaagtgg cgtcagactt cagcccccaa agcctgcgca
     1801 tcgcccacgc catcggggtg ctgcccttgc tggccgatca catccatcgt acgcaggtcc
     1861 catccgccga acgcaacctg cagcaacgcg tggacctgct ggagcagcgc tcgctgatcg
     1921 cgcaccacgt cgacatggcc tcgttggaga tcagtgccat cgcctcggaa ctggattgtg
     1981 aggaagaacg gatcagccag ctggccgggt acctcaaggg tttggaggac aagaaggagt
     2041 cgcagctcac ggttgcggcc atcgccgtgg gcgcactggg cacgctggtc acgggcgtcc
     2101 atctggccga cgagggcgac agcaatatcg atggcgcagg catcggattc gggatcgccg
     2161 gggccctgct gggcacggcc atgctcttta ccgagaagcg caccgacttc cgtcacccgc
     2221 gcaatgccat cggggtcatt catgcgggca ccgatccgca gggcatcttc ccgccatcgg
     2281 tctggta
//
LOCUS       DAOQOC010000447         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29239_length_2284_cov_2.452221, whole genome shotgun sequence.
ACCESSION   DAOQOC010000447 DAOQOC010000000
VERSION     DAOQOC010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29239_length_2284_cov_2.452221"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..>2284)
                     /locus_tag="PL106_07895"
     CDS             complement(110..>2284)
                     /locus_tag="PL106_07895"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNA33030.1"
                     /translation="VELLKSKFFIAKALQRLPLEVGYFFKGNILTNEYYTHSPFLVDD
                     LVVLDEAVRGLQITVDMGEDGNGRLHYFLDGGEYSVGYTVGVPVATPHFRCVIRPRKG
                     ERVKPPEVDGTLYIIINHPKALVARFRGQLLAGIQDQNAKTVFITCRDNNQYLARDVA
                     QAMADAFIEYDLERKSESAQSIVRFIQSQKDTVFAQLKDSEIRLQRFRKDNRVSDMEQ
                     LLPLMIERSNEYENRILELEIEDNLLDQIERSTNTDPDKMDVYELVPLLVGTSYEETL
                     SSLIDKLEDLLNERREALMDVKPNNMAAQEIEKQINLQKKLIMASIGTLRKRLAGKRA
                     DYEGRIAEFEGRFVTLPEKQMAYSRIQRLFNINEKYYIDLLEKDIEYRISKAGYVPDN
                     RVLEEAPLPTSPVSPNRNMVMVMYILTGLILGMLIVLVRYILHDNITSLNDIAKQSNA
                     SIGILGMVPKYKKEIPVSQLLIDKNPKSLIAESFRSVRTNLQFVDNSEGPKVIAITST
                     ISGEGKTFVAINLAGIISFGGKRVIVLDLDMRKPKIHLGFGVENVKGMSTLLMGKDTI
                     EDCVQRSTLKGLDFITAGPIPPNPSELILGSRMSEIIALLKTSYDVILVDNPPVGLVT
                     DGISMIQAADYPIYIFRADYSKKVFVHNVDRLINENRIRKLSAVLNGVDVDRNKYGYN
                     YGYGYGYGYGYGYGYYEDKHGPGGVRRKEGWWARFTGSKKKR"
BASE COUNT          391 a          709 c          661 g          523 t
ORIGIN      
        1 gcaccgaacg ttgcggcgtt gaacgattcg atgaaccagc cgcggtcgtc ccggaacacc
       61 ttcggccgca acaccagcag cccatcgagc ggtgcgtgtt caacctccat catcgcttct
      121 tcttcgaccc ggtgaagcgt gcccaccagc cctcttttcg tcgcacgcct cctggtccat
      181 gcttgtcctc gtaatagccg tagccgtagc cgtagccgta gccgtagccg tagccataat
      241 tatacccgta cttgttccgg tccacatcca ccccgttgag cacggcgctc agtttccgga
      301 tccggttctc gttgatcagg cggtccacgt tgtgcacgaa gaccttcttg gaataatcgg
      361 cgcggaagat gtagatcgga tagtcggccg cctggatcat gctgatgcca tcggtcacca
      421 ggcccaccgg cgggttatcg accaggatga cgtcgtaact ggtcttcagc agagcgatga
      481 tctccgacat cctggatccg aggatcaatt cgctcggatt gggcggtatg gggccggcgg
      541 tgatgaaatc gagccccttc aatgtgctcc gttgcacgca atcctcgatg gtgtccttgc
      601 ccatcagcag ggtgctcatc cccttcacgt tctccacacc gaaccccagg tggatcttgg
      661 gtttgcgcat gtcgaggtcg aggacgatca cgcgcttgcc accgaagctg atgatcccgg
      721 ccaggttgat cgccacgaag gtcttgccct ccccgctgat ggtgctggtg atggcgatga
      781 ccttgggccc ctcgctgttg tccacgaact gcaggttggt gcgcacgctc cggaagctct
      841 ccgcgatcag gctcttggga ttcttgtcga tgagcagctg gctcaccggg atctccttct
      901 tgtacttggg caccatgccc aggatgccga tactggcatt gctctgcttg gcgatgtcgt
      961 tcagcgaggt gatgttgtcg tgcaggatgt atcgtaccag cacgatgagc atgcccagga
     1021 tcaggccggt gaggatgtac atcaccatga ccatgttccg gttgggcgac acaggagagg
     1081 tgggcagcgg cgcttcctcg agcacccgat tgtccggtac gtacccggcc ttgctgatgc
     1141 ggtactcgat gtccttctcc agcaggtcga tgtagtattt ctcgttgatg ttgaacagcc
     1201 gctggatgcg cgaatacgcc atctgcttct cgggcagggt gacgaagcgg ccctcaaact
     1261 cggcgatccg cccctcgtaa tcggcccgct tgccggccag ccgcttgcga agcgtgccga
     1321 tgctcgccat gatcagcttc ttctggaggt tgatctgctt ctcgatctcc tgggccgcca
     1381 tgttgttcgg cttcacatcc atgagggctt cgcgccgctc gttgagcagg tcctccagtt
     1441 tgtcgatcag ggaggagagc gtctcttcgt aggaggtgcc caccagtaac ggcaccagct
     1501 cgtacacgtc catcttgtcc gggtcggtgt tcgtgctccg ctcgatctgg tccagcaggt
     1561 tgtcctcgat ctccagctcc aggatcctgt tctcgtactc gttgctgcgt tcgatcatca
     1621 gcggcagcaa ctgctccatg tcactcacgc ggttgtcctt ccggaaccgt tgcaggcgga
     1681 tctcgctgtc cttcaattgg gcgaaaacgg tgtccttctg gctctggatg aagcgcacga
     1741 tgctctgggc gctctcgctc ttgcgctcca gatcgtactc gatgaacgcg tccgccatgg
     1801 cctgggccac atcacgcgcc agatactggt tgttgtcccg gcaggtgatg aagaccgttt
     1861 tggcgttctg gtcctggatg ccggccaaca actgaccgcg gaagcgcgcc accagggcct
     1921 tcgggtggtt gatgatgatg tacagcgtgc cgtcgacctc gggaggcttc acccgttccc
     1981 ctttacgtgg gcggatgaca cagcggaaat gcggcgtggc caccgggacc cctaccgtgt
     2041 atccgaccga gtactccccg ccgtccagga agtaatgcag cctgccgttg ccgtcctccc
     2101 ccatgtccac ggtgatctgc agcccgcgca cggcctcgtc caggaccacc aggtcgtcca
     2161 ccaggaaggg gctgtgcgtg taatactcgt tggtgaggat attccctttg aaaaaataac
     2221 cgacctccaa cggcagccgc tggagcgcct tggcgatgaa gaatttcgac ttgagcaact
     2281 ccac
//
LOCUS       DAOQOC010000448         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29284_length_2282_cov_4.234396, whole genome shotgun sequence.
ACCESSION   DAOQOC010000448 DAOQOC010000000
VERSION     DAOQOC010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29284_length_2282_cov_4.234396"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..796)
                     /locus_tag="PL106_07900"
     CDS             complement(206..796)
                     /locus_tag="PL106_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNA33031.1"
                     /translation="MGSNRIEAFSDGMMAIFITIMVLEFKVPEGTSARDLLPLLPNVL
                     SYVLSFIYVGIYWNNHHHLFHTVTHVSGAMLLANLHLLFWLSLIPFTTAWVGENHLAP
                     VPTALYGSVLLMAASAWGILQRMIIRKQGPGSILKKALGNDLKGNLSGVAYLMAIIAA
                     FWVPVVSVVIYALVALVWIIPDRRFERVLAEAEGGE"
     gene            complement(917..>2282)
                     /locus_tag="PL106_07905"
     CDS             complement(917..>2282)
                     /locus_tag="PL106_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33032.1"
                     /translation="AHATTWTVGPDQSWTLPSQVSTLVGDGDTVNILAGVYPSDVARW
                     QADDLVLRGVGGFAHLESNGLSWGDKAIWVIQGNNCTVEWIEFSECQVPDHNGAGIRL
                     EGLNLTVRHCWFHHNENGILCGEYHPSTVRIEHSEFGHHGFGDGYSHNLYIGNIDSLI
                     FRYNYSHHADVGHELKSRAWVNVIEYNRFSNEDDGTASREIDLPNGGQAYLIGNVIQQ
                     GLQSQNSNLVGFGMEGLINPGPQELYAVNNTLVNEKVNGSFFQSPPEVYIKAYNNVLA
                     GGGEFMSAWPDDMDTLANLRITDIIAADFADPDDYDYHITGSSPAFGIGQPAGLGSSG
                     YPLVALYEYVHPANSVLRCQHALLDAGAFEACPTGMYAMDGPDVELFPNPATEQLTIR
                     AASNANITGIELTDTRGRMVARATGPARQRWDLDLHGIVPGHFTARITTSTGSRLMPF
                     VKLP"
BASE COUNT          445 a          674 c          712 g          451 t
ORIGIN      
        1 ggtgggccag gcccattcgg ggcagtgcat cacccatccg ttgccgcctt gctcaaaacc
       61 gggatcggtg aactgcgcgc aagcaccgac cgggaagagg ccgatgagca agatcggcgc
      121 aagcagtttc ttcatgggtg gaagacggga tgaacgtgtt cgggttgccg tgtaccgtac
      181 acctgatgcg gatccggtag gccgctcact ccccgccctc cgcttctgcc agcacccgtt
      241 caaatcggcg atcgggaatg atccagacca aggccaccag cgcgtagatc acgacggaga
      301 ccacaggcac ccagaaggct gcgatgatgg ccatcaggta tgccacaccg gaaagattgc
      361 ccttgagatc attacccaga gctttcttga ggatggagcc ggggccttgt ttccggatga
      421 tcatgcgctg caggatgccc caggccgatg ccgccatcaa cagcacggac ccgtacagtg
      481 cggtgggcac gggagccaaa tgattctcgc ccacccacgc cgtggtgaaa gggatcaacg
      541 agagccagaa gagcaggtgc aggttggcca agagcatggc cccgctcaca tgcgtcaccg
      601 tgtggaacag gtggtggtgg ttgttccagt agatgcccac gtagatgaag ctgagcacgt
      661 aactgagcac gttgggcagc aggggcagca ggtcccgcgc cgatgtgccc tcgggtacct
      721 tgaactccag caccatgatg gtgatgaaga tggccatcat accgtcgctg aaggcttcta
      781 tgcggttgga tcccatggtg agcgtatgga atgatgaaca gggtctggag gacgaacgat
      841 agggccggtt cccgaagcgg atccgccggg gtcgaatatc ggaggagacc ggcaccgatc
      901 tctcgtccat gcgtggtcag ggcagcttca cgaagggcat gagccgtgat ccggtggaag
      961 tcgtgatccg tgcggtgaag tgcccgggaa cgatgccgtg caggtccaga tcccaacgtt
     1021 gccgggccgg cccggtggca cgggccacca tgcggccacg cgtatcggtc agttcgatcc
     1081 cggtgatgtt cgcgttggaa gccgcacgaa tggtcagttg ctccgtggcc gggttcggga
     1141 atagctccac atccggtcca tccatcgcgt acatgccggt cgggcatgcc tcgaaggccc
     1201 ccgcatcaag caacgcgtgc tggcacctca ataccgaatt cgcaggatgg acgtactcgt
     1261 acagggcgac caggggatag ccgctgctgc caaggcctgc cggctgtccg atgccgaatg
     1321 cgggagaact cccggtgatg tggtagtcat agtcgtccgg atcggcgaag tccgctgcga
     1381 tgatgtcggt gatgcgcagg ttggcgagcg tgtccatgtc gtcaggccac gcggacatga
     1441 attcaccacc accggccagc acattgttgt atgctttgat gtacacctcg ggtggggatt
     1501 ggaagaaact gccattcacc ttctcgttca ccagcgtatt gttcaccgcg tacagttcct
     1561 gcggtcctgg attgatcagg ccctccatgc cgaagccgac caggttgctg ttctggcttt
     1621 gcaagccttg ttggatcacg tttccgatca gataggcctg tccgccgttg ggcaggtcga
     1681 tctcgcggct cgcagtaccg tcatcctcat tactgaagcg gttgtactcg atcacgttca
     1741 cccaagcgcg gctcttcagt tcatggccca catcggcgtg gtggctgtaa ttgtaccgga
     1801 agatgagcga gtcgatgttg ccgatgtaca ggttgtgcga atagccatcg ccgaagccat
     1861 gatggccgaa ttcgctgtgt tcgatgcgaa cggtgctcgg atgatactca ccgcacagga
     1921 tcccgttctc gttgtggtgg aaccagcagt gccgcacggt gaggttcaac ccttccagcc
     1981 ggatacccgc gccgttgtgg tccggcacct ggcactcgct gaactcgatc cattccacgg
     2041 tgcagttgtt cccctggatc acccagatgg ctttgtcgcc ccaactcagg ccgttgctct
     2101 ccaggtgtgc gaagccgccc acaccgcgca gcaccagatc atcggcctgc cagcgcgcca
     2161 catcgctcgg gtacacaccc gctaaaatgt tcacggtatc gccgtcccct acgagcgtgg
     2221 agacttggct gggcagtgtc caggactggt ccggtccaac ggtccacgtg gtggcatggg
     2281 cc
//
LOCUS       DAOQOC010000449         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29286_length_2282_cov_4.101482, whole genome shotgun sequence.
ACCESSION   DAOQOC010000449 DAOQOC010000000
VERSION     DAOQOC010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29286_length_2282_cov_4.101482"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PL106_07910"
     CDS             complement(<1..955)
                     /locus_tag="PL106_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNA33033.1"
                     /translation="MSAHPDTDKRLFLLDAFALIYRAYFSFIRAPRVNSKGFNTSAAF
                     GFTTTLLDLIKRERPTHLAVVFDTSAPTERHETLLDYKANREEMPDDIRSNLPYIRRI
                     IEALNIPILESDGYEADDVIGTLAKKAELEGYTTFMVTPDKDFGQLVTDRIIMYKPGR
                     GGDPPEKLGPKEIRDRWGGLSSTEQVKDILGLMGDAVDNIPGIPGIGEKTAIKLVQQF
                     GSLEGVIDGVEQLKGKQKENVIQFAEQGRMSKMLATINTDAPVDLDHDALHLDPPDKD
                     KVMEVFSELEFRTLAARVLSPADAGSADAGPKSNGATGRRGS"
     gene            complement(993..1583)
                     /locus_tag="PL106_07915"
     CDS             complement(993..1583)
                     /locus_tag="PL106_07915"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNA33034.1"
                     /translation="MFTGIVEGTGEVTGLSRAGSNLELVVRANITGELRIDQSVSHNG
                     VCLTVVELMGDRYRVTAVEETLQRSNLGDLKPGDPVNLERCLRLGDRLDGHMVQGHVD
                     AVTTCTAVEDRDGSWWFSFALPEQKRLLVEKGSVCLNGVSLTIAQLTDRDFSAAIIPY
                     TFAHTNFHALRPGHRVNVEFDVLGKYVERIMHLRAG"
     gene            complement(1583..>2282)
                     /locus_tag="PL106_07920"
     CDS             complement(1583..>2282)
                     /locus_tag="PL106_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33035.1"
                     /translation="YVRTTGAYSDPSIVAPLLNFSQIVCASPVDLTPPCPPTVSLDND
                     CETPLNSLTWNNPNESCADDTYQYHIWFTPSFDEPLQVIATLIGAENTFYQHTDGQSV
                     AGCYAVSAIDTVGNESLLSDTVCGDNCPTYELPNVFSPNSDGTNDLFVPFPYRGVKEI
                     DLHIYNRWGQLVFTSTDPDIEWPGTYKNAGEPVPDGVYYYTCDVMLRRLAGDEPLRLK
                     GYVHVLRGNQARIN"
BASE COUNT          382 a          695 c          723 g          482 t
ORIGIN      
        1 tggacccgcg ccgaccggtg gccccgttgc tcttcggacc cgcatcggcg ctacctgcat
       61 ccgcagggct gagcacacgg gcggccagtg tcctgaactc cagctcactg aagacctcca
      121 tcaccttgtc cttgtccggc ggatcgaggt gcaacgcatc gtggtcgaga tccaccggcg
      181 cgtcggtgtt gatggtggcc agcatcttgc tcatccggcc ttgttccgcg aactggatca
      241 cgttctcctt ctgtttgccc ttcagttgct ccaccccgtc gatcacgcct tccagtgagc
      301 cgaactgctg caccagtttg atggcggttt tctccccgat acctggtatg cccggtatgt
      361 tgtccaccgc atcgcccatg aggccgagga tatccttgac ctgttcggtg ctgctgagcc
      421 cgccccagcg atcacggatc tccttgggcc cgagtttctc cggtggatcg ccaccgcgac
      481 cgggcttgta catgatgatc cggtccgtaa cgagctgccc gaagtccttg tccggtgtca
      541 ccatgaaggt ggtgtagcct tcgagttcgg ccttcttggc cagggtgccg atcacatcgt
      601 cggcctcgta accatcgctc tcgaggatgg ggatgttgag cgcctcgatg atgcggcgga
      661 tgtatggcag attgctgcgg atgtcgtcgg gcatttcctc gcggttggcc ttgtagtcca
      721 gcaaggtctc gtgccgttcg gtgggcgcag aggtatcaaa gaccaccgcg aggtgggtgg
      781 gtctttcccg cttgatcagg tccagcaggg tggtggtgaa accgaaggcc gcactggtgt
      841 tgaagccctt gctgttcacg cgcggtgcgc ggatgaaact gaagtaggcg cggtagatca
      901 gcgcgaaggc gtcgagcagg aagaggcgct tgtcggtatc ggggtgggcg gacatgtcgc
      961 gaagatggcg tgatccgctc cacgctcgct gctcatccgg cccgcaggtg catgatcctc
     1021 tccacgtact tccccagcac gtcgaattcc acgttcaccc gatggcccgg gcgcagtgca
     1081 tggaaattgg tgtgtgcgaa ggtgtagggg atgatcgccg cgctgaagtc gcggtccgtc
     1141 aactgggcga tggtcaggct cacgccgttg aggcagacgg agcccttctc caccagcagg
     1201 cgcttctgct cgggcagcgc gaaggagaac caccagctgc cgtcgcggtc ttccacggcc
     1261 gtacaggtgg tcacggcgtc cacgtgcccc tgcaccatgt ggccgtccaa acggtcgccc
     1321 aggcgcaggc aacgttcaag gttcacgggg tcgccgggct tcaagtcgcc gaggttgctg
     1381 cgttgcaggg tctcttccac ggcggtcacg cgatagcggt cgcccatcag ctccactacc
     1441 gtcaagcaca ccccgttgtg gctcacactc tggtcgatgc gcaactcacc ggtgatgttc
     1501 gcgcgcacga cgagttcaag attcgaaccg gcccgcgaca gcccggtcac ttcacccgtc
     1561 ccctccacga tgcccgtgaa catcagttga tacgggcctg gttgccgcgc agcacatgca
     1621 cgtagccctt caagcggagc ggttcgtcac cggccaggcg tcgtaacatc acatcgcagg
     1681 tgtagtaata cacaccatcg ggcaccggct caccggcgtt cttgtaggtg cctggccact
     1741 cgatgtccgg atcggtactg gtgaacacga gctggcccca acggttgtag atgtgcaggt
     1801 cgatctcttt cacaccacgg taggggaaag ggacgaagag gtcgttcgtt ccgtcgctgt
     1861 tgggcgagaa tacgttgggc agttcgtatg tcgggcaatt gtcgccgcac accgtatcgc
     1921 tcaacaggct ttcgttgcct acggtatcga tcgctgaaac ggcatagcag ccggccacgc
     1981 tctgtccgtc ggtatgctgg taaaaggtgt tctccgcgcc gatcagcgtg gcgatcacct
     2041 gcaacggctc atcgaacgag ggcgtgaacc agatgtggta ctggtacgtg tcgtcggcgc
     2101 aactctcgtt cgggttgttc caagtgagcg agttcagcgg tgtctcgcaa tcgttgtcca
     2161 ggctcaccgt gggcgggcaa ggcggtgtca ggtccaccgg gctggcgcac acgatctggc
     2221 tgaagttgag gagcggtgct acgatggacg ggtcgctgta ggctcccgtg gtgcgcacgt
     2281 ac
//
LOCUS       DAOQOC010000450         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29293_length_2282_cov_2.760216, whole genome shotgun sequence.
ACCESSION   DAOQOC010000450 DAOQOC010000000
VERSION     DAOQOC010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29293_length_2282_cov_2.760216"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PL106_07925"
     CDS             <1..1130
                     /locus_tag="PL106_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA33036.1"
                     /translation="TLSPVLLGLLNDEVEVDKIEEASALRAHIGKMLEGLAAHAVVKK
                     QTVDKEDIAAVVSHKTGIPIGKVQTQEKERLMSIEEILKKRVVGQDHAIKILGDAILE
                     SRSGLNKAGTPIGSFFFLGPTGTGKTELAKALAEFLFSDENSLIRFDMSEFKEEHSAA
                     LLYGAPPGYVGYEEGGLLVTKIRQQPYAIVLFDEIEKAHPSVFDLFLQILDEGKLHDR
                     LGREGDFSNAVILFTSNIGSEHIIEEFAAGRIPKSSELLEIMGSHFRPEFLARLTEIL
                     PFRPISEETVESIFNLQLKQLEKALEKQNITLRLTDGTRRALALEGFTPRYGARPLRG
                     VIRNRLRRPISRMIISQAVTKGQTIVAHMNDGELELTVENQ"
     gene            1143..1601
                     /locus_tag="PL106_07930"
     CDS             1143..1601
                     /locus_tag="PL106_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33037.1"
                     /translation="MAEFEYGIGGTERPVDASEAFADLPQNKTLMIEKLTADGAAKPQ
                     IATGLTNVEAVFEHFKPKVEMEFEGEDGATVKEDLKFKNLGDFGSKGLTRQSGFLQGL
                     NLKQNEMNKIVKQLRSNKRFVTAMQDPAAKAAFVEVLKGLAAEIDAADKH"
     gene            1634..>2282
                     /locus_tag="PL106_07935"
     CDS             1634..>2282
                     /locus_tag="PL106_07935"
                     /inference="COORDINATES: protein motif:HMM:NF036663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     protein TssC"
                     /protein_id="HNA33038.1"
                     /translation="MSNEPQKDTRPAEKVREGVKENLDTLTRFGGFDLFESVVDGIQN
                     VNPESKARRKIFLNEGNYAAERKQLKEKLQLWLDTLSSSDNVADIIRACEEKSETTER
                     VYRENMRKALEATSELERSYRSVALFYKNTESDKLKNVNIMNASMDVLQDLDNTTFID
                     AVEQEFKDNYDRLDLRDNYGILVLPGYLGSNKVVEKWAKFCHKNKVTMVTDFQHLD"
BASE COUNT          547 a          686 c          697 g          352 t
ORIGIN      
        1 gcaccctgag cccggtattg ctgggcctgc tgaacgatga ggtggaagtg gacaagatcg
       61 aagaggcatc ggcgctccgt gcccacatcg gcaagatgct ggaaggtctg gccgctcacg
      121 ccgtggtgaa gaagcagacc gtggacaagg aggacatcgc ggcggtggtc tcgcacaaga
      181 cgggcatacc catcggcaaa gtgcagaccc aggagaagga gcgcctcatg agtatcgagg
      241 agatcctcaa gaaacgtgtg gtgggccagg accatgcgat aaagatcctg ggcgatgcca
      301 tcctggagag ccgctccggg ctgaacaaag caggcacgcc gatcggttcc ttcttctttc
      361 tgggacccac cggcaccggc aagaccgagc tggccaaggc cctggccgag ttcctgttca
      421 gcgacgagaa cagcttgatc cgcttcgaca tgagcgaatt caaggaggag catagcgcgg
      481 ccctgctgta cggtgcacct ccgggttacg tagggtacga ggaagggggc ctgctggtca
      541 ccaagatccg ccagcaaccc tacgccatcg tgttgttcga cgagatcgag aaggcgcacc
      601 catcggtgtt cgacctcttc ttgcagatcc tggacgaggg caagctgcac gaccggctgg
      661 gccgcgaggg cgacttcagc aacgcggtga tcctcttcac gagcaatatc ggcagcgagc
      721 acatcatcga ggagttcgcc gctggtcgca taccgaagtc cagtgagttg ttggagatca
      781 tgggcagcca cttccgcccc gaattcctgg cgcgcctcac cgagatcctt cccttccgcc
      841 cgatcagcga ggaaacggtg gagtccatct tcaacctgca attgaagcag ttggaaaagg
      901 ccttggagaa gcagaacatc accctccgcc tcaccgatgg cacgcgccgc gcgctggccc
      961 tggagggctt caccccgcgc tacggcgcac gtccgctgcg gggggtcatc cgcaaccgcc
     1021 tgcgccgacc gatctcgcgc atgatcatca gccaggccgt taccaaaggc cagaccatcg
     1081 tggcccacat gaacgatggg gagctggaac tcaccgtaga aaaccaataa ccaaccctga
     1141 acatggcaga attcgagtac ggcatcgggg ggaccgaacg accggtcgat gcgagcgagg
     1201 ccttcgctga cctcccccag aacaagacgc tgatgatcga gaagttgacg gcggatggcg
     1261 cggccaagcc gcagatcgcc accggcctca ccaacgtgga ggcggtgttc gagcacttca
     1321 agcccaaagt ggagatggaa ttcgagggcg aggatggcgc cacggtgaag gaggacctga
     1381 aattcaagaa cctcggcgac ttcggctcca agggtctcac gcgccagagc ggcttcctgc
     1441 aggggctcaa cctcaaacag aacgagatga acaagatcgt gaagcaattg cgcagcaaca
     1501 agcgcttcgt caccgccatg caggatcctg ccgcgaaggc cgccttcgtg gaggtgctca
     1561 aaggcctggc cgccgagatc gacgcggccg acaaacactg acgcggaccg cgctaaaaat
     1621 accacccgac cccatgagca acgagcccca aaaggacacg cgcccggccg agaaggtccg
     1681 cgaaggcgtg aaggagaacc tggataccct gacccgcttc ggcggcttcg acctcttcga
     1741 gagcgtcgtg gacggcatcc agaacgtgaa cccggagagc aaggcccgcc gcaagatctt
     1801 cctgaacgaa gggaactacg cggccgagcg caagcaactc aaggagaagc ttcagctctg
     1861 gttggacacg ctgagttcct cggacaacgt ggcggatatc atccgcgcct gcgaggagaa
     1921 gagcgagacc accgagcgcg tgtaccgcga gaacatgcgc aaggcgctgg aagccaccag
     1981 cgaactcgaa cgttcctacc gcagtgtcgc actcttttac aagaacaccg agagcgacaa
     2041 gctgaagaac gtgaacatca tgaatgcgtc catggacgtg ctgcaggacc tggacaatac
     2101 caccttcatc gatgcggtgg aacaggagtt caaggacaac tacgaccggc ttgacctgcg
     2161 cgacaactac ggcatcctgg tacttcccgg ctacctgggc agcaacaagg tggtggagaa
     2221 atgggccaag ttctgtcaca agaacaaggt gaccatggtg acggatttcc agcacctgga
     2281 tt
//
LOCUS       DAOQOC010000451         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29351_length_2279_cov_3.232464, whole genome shotgun sequence.
ACCESSION   DAOQOC010000451 DAOQOC010000000
VERSION     DAOQOC010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29351_length_2279_cov_3.232464"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1267
                     /locus_tag="PL106_07940"
     CDS             101..1267
                     /locus_tag="PL106_07940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA33039.1"
                     /translation="MNTDRRLRIGYVTVDDPHDRRTWSGTNFYLKQALEARYDVVPLG
                     PLEPQPELFMARALNQATLKIIKKRFNYRDSFLMARAYRRLLRRRLAHERLDLLVAPA
                     GLSTVALLDTDLPIIHINDRCLAGALGYHHILRDLLPFSQRDGLALEQRTLHKAAWNV
                     YASDWAADAARRIAPDVGDRILTIPFGVNFPELPPPPVPRSFPDGPLRLLFIGTKWEE
                     KGGPIAWDTLLELKGRGIAATLTVCGCVPPADLRDPDLVRAGFLDKNDPVQLARLQEL
                     LRTADLLIVPTRFEAYGIVFCEAAAYGVPALATRTGGVPTIIEDGVTGFMFDPDRTGS
                     AYADRIIGLVQRPEHWQAMRKASRLRFEQRFTWRAFVDTLSGQWGPAGLISRSR"
     gene            complement(1228..>2279)
                     /locus_tag="PL106_07945"
     CDS             complement(1228..>2279)
                     /locus_tag="PL106_07945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA33040.1"
                     /translation="LNTGGAEQVHADIMVTVREPRPIIFITGSSKDRGFADRFAACGT
                     VVEIPHLLHHPCTARSAQRRIVAALNAREQPVLFGANTDHFALWGAQLKPGARLIHLI
                     HAFLHQPSGNRKHKAWLPLFARMDRYVFVADQARREFEGFLFGNGLPRTAFGKLTTIP
                     NAVHGFGQVEEHAVPGILFVGRDSAEKRLDLFLRIADELHRTLPGKYRFTVMGARIRP
                     GHPHVHFRGTITDAAARDNVYRANDVLVLTSDREGFPMVIMEGMAQGLAIVATPVGDI
                     PERVEPGFGMISSSTARETVIQEMVAGLGALAQDPERMIRMRHAALEHARGAFGMERF
                     RERYRDLLMSPAGPH"
BASE COUNT          400 a          726 c          728 g          425 t
ORIGIN      
        1 gggccgtgga gagcatgctc gaagtgaagg cgggtaccga tatccatgca tgggccctgc
       61 tgaccttcgc gttcatgcgt aacgtgcgaa cctcaaccgc atgaacacgg accgtcgcct
      121 gcgcatcggc tacgtcacgg tggatgatcc acatgaccgg agaacgtggt ccggcaccaa
      181 tttctacctg aaacaggccc tggaggcgcg gtatgacgtg gtgccactgg ggccactgga
      241 gccgcagccc gagctgttca tggcccgtgc cctcaatcag gccacgttga agatcatcaa
      301 aaaacggttc aattaccgcg actcgttcct gatggcgcgc gcttaccgcc gtctattgcg
      361 gcgcaggttg gcccatgagc gcctggacct gttggtggcc ccggccggcc tgagcacggt
      421 ggccttgctg gatacggatc tgcccatcat ccacatcaac gaccggtgct tggccggtgc
      481 gctggggtac caccacatcc tgcgcgatct gctgcctttc tcgcaacgcg acggactcgc
      541 cttggaacag cgcacgttgc acaaggccgc atggaacgtc tatgcttcgg attgggccgc
      601 ggacgccgca cgccgcatcg ctccggatgt gggggacagg atcctcacga tcccgttcgg
      661 ggtgaacttt ccggagcttc cgccaccacc tgtgccgcgt tcgttccccg atgggccgtt
      721 gcgcctgctc ttcatcggca ccaaatggga agagaaggga gggcccatcg catgggacac
      781 attgctcgaa ctcaaggggc gtgggattgc cgccacactc accgtatgcg gatgcgtgcc
      841 gccagcggac ctccgggatc cggacctcgt gcgtgcgggt ttcctggaca agaatgaccc
      901 ggtgcaactc gcccggctgc aggagttgct tcgcacggcc gacctgctca tcgtccccac
      961 acgcttcgag gcctacggta tcgttttttg cgaagccgca gcgtacgggg tgcctgcgct
     1021 ggccacgcgt acgggtggcg tccccaccat catcgaggat ggcgtcacgg gtttcatgtt
     1081 cgatcctgat cgaacgggat cagcctacgc ggatcgtatc atcgggctgg tgcaacggcc
     1141 cgaacactgg caggccatgc gaaaggcgtc ccgtctgcgc ttcgaacagc ggttcacgtg
     1201 gcgtgcattc gtggataccc tgtcaggtca atggggtcca gcgggactca tcagcaggtc
     1261 ccggtagcgc tcgcggaaac gctccatgcc gaacgcgcca cgggcatgtt ccagcgcagc
     1321 atgccgcatg cggatcatgc gttcggggtc ttgggccagc gcgcccaacc cggccaccat
     1381 ttcctggatc accgtctccc gggccgtaga tgaactgatc atgccgaagc cgggttccac
     1441 gcgttccggg atgtccccga ccggcgtggc cacgatcgcg agcccttggg ccatgccttc
     1501 catgatcacc atggggaagc cttcccgatc ggaggtgagc acgagcacgt cgttggcccg
     1561 gtacacgttg tcgcgcgcag cggcatcggt aatggtgccc cggaaatgca catgcggatg
     1621 gccgggcctg atccgggcgc ccatgaccgt gaaccggtat tttccgggca gggtccggtg
     1681 cagctcgtcg gcgatgcgca agaagaggtc cagtcttttc tccgccgagt cgcgtcccac
     1741 gaacaggata cccggaaccg catgctcctc cacctggccg aaaccatgca cggcattggg
     1801 tatcgttgtc aacttgccga aggccgtgcg cggaaggccg ttcccgaaga ggaagccttc
     1861 gaactcccga cgggcctgat cggccacgaa cacgtagcgg tccatccggg cgaaaagtgg
     1921 aagccaggcc ttgtgcttcc tgttgcccga tggctggtgc aggaacgcat ggatcaggtg
     1981 tatgagccgg gcgccgggtt tcagctgtgc gccccacagg gcgaagtggt cggtattcgc
     2041 gccgaacagc acgggctgct cacgtgcgtt gagcgccgcc acgatccgtc gttgggcgct
     2101 tctggcggta cacgggtgat gcagcaggtg agggatctcc accacggtgc cgcatgccgc
     2161 gaaacgatcg gcgaagccac ggtccttgct cgatccggtg atgaagatga tcggccgcgg
     2221 ctcgcgcacg gtgaccatga tgtcggcatg cacctgttcg gcccctccgg tattcaaat
//
LOCUS       DAOQOC010000452         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29372_length_2278_cov_3.333783, whole genome shotgun sequence.
ACCESSION   DAOQOC010000452 DAOQOC010000000
VERSION     DAOQOC010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29372_length_2278_cov_3.333783"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..507)
                     /locus_tag="PL106_07950"
     CDS             complement(148..507)
                     /locus_tag="PL106_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNA33041.1"
                     /translation="MKTQDYARVFAYSFASIYPMYVQKVEKKGRTKAELDQVLCWLTG
                     YDTKGLQKQIASKCDLKTFFAAAPRMHPNATQVTGMICGVCIEEIENPLMRDIRRMDK
                     LVDELAKGRAMEKILRA"
     gene            complement(504..908)
                     /locus_tag="PL106_07955"
     CDS             complement(504..908)
                     /locus_tag="PL106_07955"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNA33042.1"
                     /translation="MPTGTVVSWLAVVLFCSGLLLFATTLAAFVKRGNGTLAPWTPTQ
                     RLIVSGPYRYCRNPMITGVLLILCAEALWLRSGWIAVEAVIFFAVNTVYFILAEEPSM
                     EHRFGQDYRAYKADVPRWIPRLKPYFASPDIP"
     gene            complement(1016..1462)
                     /locus_tag="PL106_07960"
     CDS             complement(1016..1462)
                     /locus_tag="PL106_07960"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA33043.1"
                     /translation="MKAANTTDYLAALPIDQRKALEILRKHILAAAPGAEEYFGYGLP
                     AFKYNGHPLVYIGAAKNHCALYGAVPPGFKEKLKGFIVSKGTIQFMPEKPLPAGLAKE
                     IVKAKCAEIEVRWPVREVKKVKEVKKGKNVKVVRMVQKATKNPVKE"
     gene            complement(1489..1983)
                     /locus_tag="PL106_07965"
     CDS             complement(1489..1983)
                     /locus_tag="PL106_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA33044.1"
                     /translation="MSKLQRTQACLWFPGNAEEAVAFYMGIFPNSRIISKPVVSEEVS
                     TASGLPVGSVLCIEFELDGELFLALNGGPQFKFTEAVSLMVRCKDQQEIDHYWKKLSA
                     GGPPEAQICGWLKDKYGLSWQVVPAMLTDMMRDAKGYDRMMVALMGMKKLVIAELEKA
                     FKGK"
     gene            complement(2075..>2278)
                     /locus_tag="PL106_07970"
     CDS             complement(2075..>2278)
                     /locus_tag="PL106_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA33045.1"
                     /translation="RIGLSANTMGISVYIMGLADKDHLANTYGKRLGKASITGYCIKF
                     KNLKDINVGVLEEAIRDRAQRPK"
BASE COUNT          403 a          682 c          648 g          545 t
ORIGIN      
        1 cgttggggtt cattcagttc cggttcacca cagaggcaca gagcacacag aggaatacta
       61 tgatttcaac accaatatag caacgcaaga tccgtcctgc gctgctcggt tctctgtgtg
      121 ctctgtgcct ctgtggtgtg catttcttca agcgcgcagg atcttttcca tcgcccttcc
      181 cttggccagt tcatccacca gcttgtccat gcggcggatg tcgcgcatca gcgggttttc
      241 gatctcctcg atgcacaccc cgcagatcat gccggtgacc tgggtggcat tcgggtgcat
      301 gcgtggcgcg gcggcgaaga aggtcttcag gtcgcacttg ctggcaattt gcttttgcag
      361 gcccttggta tcgtagccgg tgagccagca gagtacttgg tccagttcag ccttggtgcg
      421 gcccttcttc tccactttct gcacgtacat ggggtagatg gaggcgaagg agtaggcgaa
      481 gacgcgggcg tagtcctggg ttttcatgga atgtctggac ttgcgaagta aggcttcagg
      541 cgcgggatcc aacgcggcac atctgctttg tacgcgcggt agtcctgccc gaatctatgt
      601 tccatgcttg gttcctcagc caggataaag tacacggtgt tcacggcgaa gaagatcacg
      661 gcttccacgg cgatccaacc tgacctcagc caaagtgcct cggcgcacag gatgagcagc
      721 accccggtga tcatcgggtt gcggcaatag cggtaggggc cggaaacgat cagtcgttgt
      781 gtgggggtcc agggcgccag ggttccgttg ccccgcttca cgaaagctgc cagcgtggtt
      841 gcgaacagca gcaagccaga acagaagagc accacggcca accatgacac aacggtacct
      901 gtcggcatcc atgctggcga ccctgcatgg atcaaccacg ggatcacgca ggtaacggta
      961 acgggaagta tgagcacgtc acgcacatgt ttccatatcg gcggtccgga cctcatcact
     1021 ccttcaccgg gttcttcgtc gctttctgca ccatcctcac caccttcaca ttcttcccct
     1081 tcttaacttc cttcaccttc ttcacctccc tcaccggcca ccgcacctcg atctccgcgc
     1141 atttcgcctt cacgatctcc tttgccagcc cggcgggcaa gggtttttcc ggcatgaact
     1201 ggatcgtgcc tttgctcacg ataaagcctt tcagcttctc tttgaacccg ggcggcaccg
     1261 cgccgtacag cgcgcagtgg ttcttggccg cgccgatgta caccaacgga tgtccattgt
     1321 acttgaaggc gggcaggccg tagccgaagt attcttccgc gccgggggct gcggcaagga
     1381 tatgcttgcg caggatttcc aaggccttgc gctggtcgat cggcagcgcg gccaggtagt
     1441 ccgttgtgtt tgcggctttc atgggttgag cgttggttgc ctgctgttct acttgccttt
     1501 gaacgccttc tccagctctg cgatcaccag cttcttcatg cccatcagcg ccaccatcat
     1561 gcggtcatag cccttggcgt cgcgcatcat gtcggtaagc atggcgggga ccacctgcca
     1621 gctcaatccg tacttgtcct tgagccagcc gcagatctgc gcttcgggtg gcccgcccgc
     1681 gctgagcttt ttccagtagt ggtcgatctc ctgttggtcc ttgcagcgca ccatgagcga
     1741 aacagcttcg gtgaacttga actgcggacc gccgttaagc gccaggaaca gctcgccgtc
     1801 cagctcgaac tcgatgcaga gcaccgaacc cacgggcagt ccgctcgccg tggatacttc
     1861 ctcggatacg accggcttgc tgatgatcct ggaattcggg aagatgccca tgtagaaggc
     1921 cacggcctct tcagcgttgc cagggaacca caggcaggct tgggtgcgtt ggagcttgct
     1981 catttgggaa ggggggtgtt gcgggtcgac ggtacggatc tgaagtttct tttcctctgt
     2041 gttctctgtg cctctgtggt gatacaccca cgacctattt cggtcgttgc gccctgtctc
     2101 ggatcgcttc ttccagcacg cccacgttga tgtccttcag gttcttgaac ttgatgcagt
     2161 agccggtgat gctggccttg ccaagtcgtt tgccgtaggt gttggcgaga tggtccttgt
     2221 ctgcaaggcc catgatgtac acgctgatcc ccatggtgtt ggcgctcaac ccgatgcg
//
LOCUS       DAOQOC010000453         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29431_length_2276_cov_2.923908, whole genome shotgun sequence.
ACCESSION   DAOQOC010000453 DAOQOC010000000
VERSION     DAOQOC010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29431_length_2276_cov_2.923908"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PL106_07975"
     CDS             <1..807
                     /locus_tag="PL106_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33046.1"
                     /translation="QYLSTLGVWAMGPIMTSKLAGSEWYYWSIQWFLHFQFNGWFWFA
                     AMAIGSRWAEKHGVHVDLDRLTVFLWVIGTLLTFALTIAWSERLPAIIAVNSLGVLVQ
                     TWAAWRTMRAMFRARLRALVRTTPWMRVVIGAMLIAMGVKVAAQTAVTIPAVADIAMT
                     LRNFVVGFVHLNTLGAGTMVLLAFALERRWLDESRRIIRFGLVALITGFALSELLLFG
                     QGTLIWVRWGMLPGYNWMLFGVSALLPTGAIMLLAGALRGARPVPVVQGS"
     gene            1171..1383
                     /locus_tag="PL106_07980"
     CDS             1171..1383
                     /locus_tag="PL106_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33047.1"
                     /translation="MRKWTPVLIENVEKKRTENWLASSELFALPGTKKLVEAEDHFGI
                     VRVYVSEHPIERPPVKSSDSTTRGRR"
     gene            1547..>2276
                     /locus_tag="PL106_07985"
     CDS             1547..>2276
                     /locus_tag="PL106_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA33048.1"
                     /translation="MKDQLTHLLHEFHLPLQNPVLVFALILTIILLAPILLKRLNIPG
                     IIGLIISGVAIGPHGLNLLDNNSAINLFSTIGLLYIMFIAGLELDLNEFKAHRNRSMW
                     FGLFTFAIPLALGYPVCRYLLGYDFNASLLTASMFATHTLVAYPIVSRLGVAKNQAVA
                     IAVGGTILTDTAVLIMLAVIIGKSHGTLDMDFWIKLLVSLALFSAVMFAVIPRIAKWF
                     FRKLENEKHSHYIFVLTVVFLAAFL"
BASE COUNT          400 a          685 c          703 g          488 t
ORIGIN      
        1 cagtacctga gcaccctggg cgtgtgggcc atggggccga tcatgaccag caagctggcc
       61 ggcagcgagt ggtactactg gagcatccaa tggttcctgc atttccagtt caatggctgg
      121 ttctggttcg cggctatggc tattggcagc cgatgggcgg agaagcacgg cgttcatgtg
      181 gacctggatc gcctcacggt cttcctctgg gtcatcggca cactgctcac cttcgccctg
      241 accatcgcct ggagcgagcg actgcccgcg atcatcgccg tcaattccct gggtgtgctc
      301 gtgcagacct gggccgcctg gcgcaccatg cgtgccatgt tccgcgcacg gctcagagcg
      361 ctggtgcgca ccacgccttg gatgcgggtg gtcatcggcg ccatgctcat cgccatgggt
      421 gtgaaagtgg ccgcccagac cgccgtgacc atccctgccg tggctgatat cgcgatgacc
      481 ttgcgcaact tcgtggtggg cttcgtccac ctcaataccc ttggcgcggg caccatggtc
      541 ctgctcgctt tcgcattgga gcgccgttgg ctcgatgagt ccaggcggat catccgcttc
      601 ggcctggtcg cgctgatcac cggcttcgcg ctgagcgagc ttcttctctt tggtcagggc
      661 acgctgatct gggtgcgttg ggggatgttg cccggttaca actggatgct tttcggggtc
      721 agcgccttgc tgcccaccgg agcgatcatg ttgctggcgg gagccctgcg gggcgcacga
      781 ccggtccccg ttgtgcaggg gagctgacga acccgttcgc gatcccgata ggggaggatg
      841 gtccggtgaa cggagctgta cgaccttcct tggcataccg gatcctcttt cggcgtggag
      901 cgttcatgat cggtgcgtag cattccctcc ggggatccgt cgataccaca ccacgaggat
      961 caggatgtcg tatgatggag gctgatcctt ccggtcccgt gcggtcatgc caactcgcag
     1021 aaaagcctga tccaattccg ctttgcggaa ctggatgaag cattttccgc actgcttcgc
     1081 cgtggaggtc gttccgttga tccagttgag caacgatccc cacggttcgt cctccgaaaa
     1141 gatggcggcg cgtctcttgt ccgaccttga atgcgcaaat ggacccctgt cctcatagag
     1201 aatgtggaaa agaagcgaac ggagaactgg ctggccagca gcgaactctt cgccctgccg
     1261 ggcacgaaga agttggtgga ggcggaggat cactttggga tcgtgcgggt gtatgtcagc
     1321 gagcacccca tagagcgacc cccagtaaaa agcagcgaca gtacaactcg gggaagaaga
     1381 tgaggcagcc cttgaagagc ctgttagcgg tggaccgcaa gagcggacga ccctgcggat
     1441 ccggctacat ttagcgcctg aggaccgact gttggaccgg ggatccggtg ctattgttgg
     1501 tcatcccggc ctggccgttc gacggtcggc catgtcatca gcacccatga aggatcaact
     1561 cacgcacctg ctccacgagt tccacctgcc cttgcagaac ccggtgctgg tgttcgcgtt
     1621 gatcctgacg atcatcctgc tggcgccgat cctgttgaaa cggttgaaca tccccgggat
     1681 catcggactg atcatttcgg gcgtggcgat cggaccgcac gggctgaacc tgctggacaa
     1741 caattcggcg atcaacctct tctccaccat cggactgctg tacatcatgt tcatcgcggg
     1801 acttgagctc gacctcaacg agttcaaggc gcaccggaac aggagcatgt ggttcgggtt
     1861 gttcaccttc gccataccgc tcgccctggg ctatccggtg tgccgctacc tgctgggcta
     1921 cgacttcaac gcgagtttgc tcaccgccag catgttcgct acccacaccc tggtggccta
     1981 cccgatcgta agccggttgg gggtggccaa gaaccaggcg gtggcgatcg ccgtgggcgg
     2041 gacgatcctg acggacaccg cggtgctgat catgctggcg gtgatcatcg gcaaaagcca
     2101 tggcacgctg gacatggact tctggatcaa gctcctggta tcgctcgcac tattctcggc
     2161 tgtgatgttc gccgtgatcc cgcggatcgc gaaatggttc ttccgcaagc tggagaacga
     2221 gaagcactcc cactacatct tcgtgctcac ggtggtgttc ctggcggcct tcctgg
//
LOCUS       DAOQOC010000454         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29504_length_2273_cov_3.570784, whole genome shotgun sequence.
ACCESSION   DAOQOC010000454 DAOQOC010000000
VERSION     DAOQOC010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29504_length_2273_cov_3.570784"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PL106_07990"
     CDS             complement(<1..320)
                     /locus_tag="PL106_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33049.1"
                     /translation="MKNACLFLFMLFAGSLPAQEHRPQVLTLGTFHFAFPNLDAQQVA
                     DSDRIDVLQQRYQSEIEDIVERMARFAPTVIVIERQPGEQHTIDSLYAAFLAGRHELG
                     RGEE"
     gene            complement(471..1463)
                     /locus_tag="PL106_07995"
     CDS             complement(471..1463)
                     /locus_tag="PL106_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33050.1"
                     /translation="MKRSTTLAFFIAIASFAHAQPVLTFTGNAPAPGSTRTLHYGPYV
                     SPGGSGANQNWDLSALTTDSTILIQHVQPGTTPNGASFPGSTVAETGAAATMFFRSAS
                     DGVYLVGSDADLVIPYTDQARYLPFPCTYQTNWTDDFASVFDSDGFNVFRSGTVDGTA
                     DGYGTVTMPFGTVDDVLRVHWHEETTDSTLLFTMQHTYDGYLYYVAGSSHPLAQLVSE
                     SVTFMGQTTTHNHAQWVADVSTQVPNDAVMPVASLYPVPTYDILNFNLPASFSGLQLI
                     SITDAQGRSVLERSIVQVDGRSGRADVSSLAPGTYHLTATDELGQRAARSFLVE"
     gene            complement(1597..>2273)
                     /locus_tag="PL106_08000"
     CDS             complement(1597..>2273)
                     /locus_tag="PL106_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33051.1"
                     /translation="ITNNQEHGIGRLTDPEIARTLRYGVGSDGRAILDFMPYYMLSDE
                     DLTAVISYLRTLPGNAHAVPADDLNFIGKAVKAFLIKPAGPLGTERPKGMRPDTTAAY
                     GEYLANSVANCVGCHTARDLMTGAFTGKPFAGWMNMEGNVPGTFFNTPNLTPEPTTGH
                     LANWTFEVFQARFRSGPVFPESPMPWASFRRMRDADLKAIWNYLQTLPPVVQETGPLK
                     APKEHG"
BASE COUNT          415 a          630 c          793 g          435 t
ORIGIN      
        1 tcctcctccc cacgccccag ctcatgccgt ccggccagga aggcggcata caacgaatcg
       61 atcgtgtgct gctctccggg ctgccgttcg atcacgatca ccgtgggcgc gaaacgcgcc
      121 atgcgctcca cgatgtcctc gatctccgac tggtagcgtt gctgaagcac atcgatgcgg
      181 tcactatcgg ccacctgctg cgcgtcgagg ttgggaaacg cgaaatggaa tgtgccgagg
      241 gtgagcactt gggggcggtg ctcctgcgcg ggcaagctcc cggcgaacag catgaagagg
      301 aagaggcagg cattcttcat gttcgtagga cggatgaagg atgttgatgt taccgatcga
      361 gcgcgggcca ggccgaatgc cgtacctgaa agtacggtgg acaggccagc atggaatgac
      421 gagcggcagc caccgctctg gtggctgccg ttcgccaacg gcacgatgga tcactccacc
      481 aggaaggatc gcgccgcgcg ctggcccagt tcatccgtgg cagtcaggtg gtaggtcccc
      541 ggtgcgagcg aggagacatc cgcacggccg gaccgaccat ccacctgcac gatcgaccgt
      601 tcgagcaccg atcgtccctg ggcatcggtg atgctgatca actgcagccc gctgaacgac
      661 gcaggaaggt tgaagttgag gatgtcgtag gtgggaacgg gatacaagct cgccacaggc
      721 atgaccgcat cattgggcac ttgagtgctc acatcagcca cccactgggc gtggttgtgg
      781 gtggtggtct ggcccatgaa ggtgaccgat tcgctgacca gttgggccag cgggtgcgag
      841 gatcccgcca cgtagtacaa atagccgtcg taggtgtgtt gcatggtgaa caacagggtg
      901 gagtcggtgg tctcttcgtg ccagtggacg cgcaatacgt cgtccacggt accgaacggc
      961 atggttaccg tgccgtatcc atccgccgtt ccatccacgg ttccgctgcg gaacacgttg
     1021 aaaccgtccg agtcgaacac cgaggcgaag tcgtccgtcc agttggtctg gtaggtgcaa
     1081 gggaagggca ggtaccgggc ttggtccgtg taggggatca caaggtcggc atcagaaccc
     1141 acgagataga cgccatcact ggcgctgcgg aagaacatgg ttgcagcggc acccgtttcc
     1201 gccacggtgg agccggggaa ggaagctcca ttgggcgtgg tgccgggttg cacatgttga
     1261 atgaggatcg tgctgtcggt cgtgagcgca ctgagatccc agttctggtt ggcgccggaa
     1321 ccgccgggcg aaacgtatgg gccgtagtgc agcgtgcggg tgctgcccgg tgcgggcgcg
     1381 ttgccagtga atgtgagcac cggttgggcg tgtgcgaagg atgcgatcgc gatgaagaag
     1441 gcaagggtgg ttgaacgttt catggggtgg tcggtggtgt tgatgaaggt gtgtgggagg
     1501 tgggttcatg gatcacggag gacgtgatcc cgatcattcg gtagatgaag agcaggtgga
     1561 gcatgcggga gcgtgagcgt gcggcgcagc gtgcgttcat ccgtgctcct tcggtgcttt
     1621 cagcggaccg gtctcctgca ccaccggagg cagcgtctgc aggtagttcc agatcgcctt
     1681 caggtcggca tcgcgcatgc ggcggaagct ggcccacggc atgggacttt ccgggaatac
     1741 tggaccgctg cggaagcgcg cctggaagac ctcgaaggtc cagttggcca ggtggcccgt
     1801 ggtgggctcg ggcgtcaggt tgggcgtgtt gaagaaggta ccgggcacgt tgccttccat
     1861 gttcatccaa ccggcgaagg gcttgccagt gaacgcgccg gtcatcaggt cacgtgcggt
     1921 gtggcaaccc acgcagttcg ccacactgtt cgccaggtac tccccgtatg ccgcagtggt
     1981 gtccggtctc atccccttcg gacgctccgt tcccaagggc cccgcgggct ttatcaggaa
     2041 ggccttcacc gctttgccga tgaagttgag gtcatcggcg ggcaccgcgt gcgcattacc
     2101 gggcagcgtg cgcaggtagc tgatcacggc ggtgaggtcc tcgtcgctga gcatgtagta
     2161 gggcatgaag tccaggatgg cgcggccatc actgcccacg ccgtagcgca gggtgcgggc
     2221 gatctccggg tccgtaagcc gaccaatgcc gtgctcttga ttgttggtga tgt
//
LOCUS       DAOQOC010000455         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29525_length_2272_cov_3.493911, whole genome shotgun sequence.
ACCESSION   DAOQOC010000455 DAOQOC010000000
VERSION     DAOQOC010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29525_length_2272_cov_3.493911"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PL106_08005"
     CDS             complement(<1..1442)
                     /locus_tag="PL106_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33052.1"
                     /translation="MYKAERYAETIREIDAQLKAAAGTTWQDSLHKYLYPYGRAYRKV
                     HNPAAGAAAAERILQLVMRRAIPRHQLEALFDLSWYYYDIGEPGQCARVDSMAVAVAD
                     ADPTMPPAQRGRARQYLAFDHSVLGEYRNSAAWALAAIAVYEKADSIPAAQWAESWTA
                     AGVANWHLGRIRDAERYYFKALDKLGDGEDPVILERKVSTYGNLGVLWQNAGDFNRAK
                     KYYNTSLQYSDRVIAKATDPMNRDEAIVNRSRTYLNLATVYHQLGDHGKARELMDLAW
                     RDRSAVLEPDDPQLLVIKDRQADLELSIGELTKAETFTRTYLAACERKFGRTSEEYIR
                     AASRLGDISRQQGLITRADSLFTLSIAAGRANTDQSTDALLAQTLQSRATMHSGAGRF
                     ADALQDLLAARLILVNIYDSLHYQVAGVDTRLAELSWEAGDPGACLRYAQSALAIVDD
                     RVQAARSGSLRTFPDPHILPDATYWRIRAAR"
     gene            complement(1541..>2272)
                     /locus_tag="PL106_08010"
     CDS             complement(1541..>2272)
                     /locus_tag="PL106_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33053.1"
                     /translation="IMKEGTLDRPTFEAIEAHVLERMPQNERAAFELRLATDADLRAE
                     VDLERENIQAVELGGVMRLLNDVAREERIREQKNHGGLRYLKYAAVLAAVASGIIWWV
                     TRPSLNEALYAEQFVPDPGLPVAMGTTNNIDFADAMVAYKLGHYAEARAKWTALHDRM
                     KANDTLDYYLASAWLADGNPKQAIPLLKPLVDRPASAFHHRAQWYLFLAFVHDGRTSE
                     ARTLGLEHDATYGERVRAILAQLPG"
BASE COUNT          353 a          696 c          785 g          438 t
ORIGIN      
        1 cgggccgcac ggatacgcca ataggtcgcg tcgggcagga tatgtggatc gggaaaggtg
       61 cgtaacgatc ccgatcgtgc ggcttggacg cgatcatcca cgatggccag agcggactgt
      121 gcgtagcgca ggcaggcacc cgggtcgccc gcttcccagg agagttccgc cagccgcgtg
      181 tccaccccgg ccacctggta atgcaggctg tcatagatgt tcaccaggat gaggcgcgcg
      241 gccagaaggt cctgcagtgc atccgcgaac cggccggcgc cgctatgcat ggtggcacgg
      301 ctttgcaagg tctgtgcaag caaggcatcg gtggactggt cggtgttggc cctgcccgca
      361 gcgatgctca gggtgaagag ggagtcggca cgggtgatga ggccctgttg cctcgaaatg
      421 tcacccaggc gtgaagcggc acggatgtac tcctcgctcg tgcgtccgaa tttgcgttca
      481 caagcggcca ggtaggtccg cgtgaacgtc tcggccttgg tcagttcgcc gatgctcagt
      541 tccagatcgg cctgacgatc cttgatgacg agcaactggg gatcgtcggg ttcgagcacg
      601 gcgctccgat cgcgccaggc caggtccatc agttcgcgcg ccttgccatg atcgcccagt
      661 tggtggtaca ccgtggccag gttcaggtac gtgcgcgagc ggttcacgat ggcctcatca
      721 cggttcatcg ggtcggtggc cttggcgatc acgcggtcgc tgtactgcaa actggtgttg
      781 tagtattttt tggcccggtt gaagtcgccc gcgttctgcc agagcacgcc gaggttgccg
      841 taggtgctca ccttgcgttc caggatgacc ggatcctcgc cgtcgcccag cttgtcgagc
      901 gctttgaaat aataccgttc ggcatcgcgt atgcggccca ggtgccagtt ggccactccc
      961 gccgcggtcc agctttcggc ccattgcgcg gcggggatgc tgtcggcctt ctcgtacacg
     1021 gcgatggcgg cgagtgccca ggcggccgag ttgcggtatt cgcccagcac gctgtggtcg
     1081 aaggccaggt attgccgggc gcggccccgt tgtgcgggtg gcatggtggg gtcggcatcg
     1141 gccacggcca cggccatcga gtccacccgg gcgcattgtc ccggttcccc gatatcataa
     1201 taataccagc tcaggtcgaa gagcgcttcc agctggtgcc gggggatcgc gcgccgcatc
     1261 accagttgga gaatgcgctc ggcggcagcg gcgccagcgg cggggttgtg caccttgcgg
     1321 taggcccggc cgtacgggta caggtacttg tggagcgaat cctgccaggt ggtccccgcg
     1381 gccgccttca gttgcgcatc gatctcgcgg atggtctccg cgtagcgttc ggccttgtac
     1441 aaggcattga tgcgggcgtg gttgcgggcg agcggatcct cgtgctgggc cgtggccagc
     1501 aggcccgaga ggaacgctgt gaagaagagg gcgagccgtg tcagccggga agctgcgcca
     1561 ggatggcccg cacgcgttcg ccgtaggtcg catcgtgctc caagcccagt gtacgggcct
     1621 cggaggtgcg cccatcgtgt acgaaggcca ggaagaggta ccactgcgcg cgatggtgga
     1681 aggccgaggc agggcggtcc accaaaggtt tcaacagcgg tatcgcctgc ttcgggttcc
     1741 catcggccag ccatgcgctg gcaaggtagt agtccaaggt gtcgttggcc ttcatccggt
     1801 catgcagtgc ggtccatttg gcccgggcct cggcatagtg ccccagtttg taggccacca
     1861 tggcatcggc gaagtcgatg ttgttcgtgg tgcccatggc caccggcagg ccgggatcgg
     1921 gaacgaactg ctcggcgtag agtgcctcgt tcagcgatgg ccgggtgacc caccagatga
     1981 tgccgctggc aacggcggcc agcacggccg cgtatttcag gtagcgcagc ccgccatggt
     2041 tcttctgctc ccggatgcgt tcttcccggg ccacatcgtt cagcaggcgc atcacgccgc
     2101 ccagttccac cgcctggatg ttctcgcgtt ccaggtccac ttcggcgcgc aggtcggcgt
     2161 cggtggcgag gcgcagttcg aatgccgcgc gctcgttctg cggcatgcgc tcgagcacgt
     2221 gcgcctcaat agcctcgaag gtcggccggt ccagggtgcc ctccttcatg at
//
LOCUS       DAOQOC010000456         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29669_length_2267_cov_2.297468, whole genome shotgun sequence.
ACCESSION   DAOQOC010000456 DAOQOC010000000
VERSION     DAOQOC010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29669_length_2267_cov_2.297468"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="PL106_08015"
     CDS             complement(<1..1900)
                     /locus_tag="PL106_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33054.1"
                     /translation="MPFTPIPGKLYRFAAVCSLILPLFAAAQTGTLKGFVYEHATGEP
                     SIFTPVNVVGTNFHATTDVEGYFSITKIPAGKYTVRIVYLGYDTLSKEVEVRAGSITT
                     EKLFLKKGAIALPDVEVTRDRQEAEAQTRVSVQRMTPNEIERVPAIGGQADLAQALQV
                     YPGMVFTGDQGGQLYIRGGSPIMNKVLMDGMVLYNPFHSIGLFSVFDNDILRNADIYT
                     GGFNAEYGGRMSSVMDITTRDGNKQRFSGKVAASTFAAKAMLEGPIAKAKTEGGGGVS
                     YLLNFKHSYLDQTSKTLYSFVDTAGLPFKFTDLYGKISMNAANGSKVNLFGFHFTDGV
                     RYRGVSDLDWKNSGGGASFVLAPSGSAILIDGVFALSNYEVTMKERDLAPRSSSINSF
                     NGGLNFKYFAGEDEIKYGVDIVGVRTDFNYFNSTGLRLTQQKISTEIAGYINYKIKIG
                     RLVLDPGMRAHYYATPAVMSWEPRFSFKLNLYDVKSTADIWPKVRVKGAVGRYSQNLI
                     AAQSDRDVVNLFYGFLTAPEDITNEMTLENGKTREIKDPIQRADHAIFGFEVDVNRRL
                     KANIEGYIKNFRQVSNLNRNKIYEDIPEFANQPDQLKKDFMMETGKAYGGDVLLTYEK
                     DHLYLWAVY"
     gene            complement(2072..>2267)
                     /locus_tag="PL106_08020"
     CDS             complement(2072..>2267)
                     /locus_tag="PL106_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33055.1"
                     /translation="IVNKITSIISKEHNVNMRSISFDSNDGVFEGKVMAFVHDADHLR
                     SLMDKLRRLPSVNMVERMDN"
BASE COUNT          382 a          668 c          706 g          511 t
ORIGIN      
        1 aatacaccgc ccacaggtac aggtggtcct tctcataggt cagcagcaca tccccgccgt
       61 aggccttgcc ggtctccatc atgaagtcct tcttcaactg gtccggctgg ttggcgaatt
      121 cagggatgtc ctcgtagatc ttgttgcgat tcaggttgct cacctgccgg aagttcttga
      181 tgtagccctc gatgttcgcc ttcaatcggc ggttcacgtc cacctcgaac ccgaagatgg
      241 catggtcggc cctctggatc gggtctttga tctcgcgtgt tttcccgttc tccagggtca
      301 tttcgttcgt gatgtcctcc ggtgcggtga ggaagccgta gaagaggttc accacgtcgc
      361 ggtcgctctg ggcggcgatc aggttctggc tgtaacggcc cacggcacct ttcacacgca
      421 ccttgggcca gatgtccgcc gtgctcttca catcatacag gttcagcttg aagctgaagc
      481 gcggctccca gctcatcacg gccggggtgg cgtagtagtg tgcgcgcatg cccggatcga
      541 gcaccaaccg accgatcttg atcttgtagt tgatgtaccc ggcgatctcg gtgctgatct
      601 tctgctgtgt gaggcgcaga ccggtgctgt tgaaatagtt gaaatcggtg cggacgccca
      661 cgatgtcaac cccgtacttg atctcgtcct cgccagcgaa gtacttgaag ttgagccccc
      721 cgttgaagct gttgatgctg ctgctgcgcg gggccaggtc gcgctccttc atggtcactt
      781 cgtagttgct cagggcgaac acgccatcga tcaggatggc gctaccgctg ggggccagaa
      841 cgaagctggc tccgccaccg gagttcttcc agtccaggtc gctcaccccg cggtaacgca
      901 ccccgtccgt gaagtggaag ccgaaaaggt tcaccttgct gccgttggcg gcgttcatgc
      961 tgatcttgcc gtacaggtcg gtgaacttga acgggagacc ggccgtgtcc acgaaggagt
     1021 acagtgtctt gctggtctga tccaggtagc tgtgcttgaa gttgagcagg tagctgaccc
     1081 cgccgcctcc ttcggtcttc gccttggcga tcgggccttc gagcatggcc ttggccgcga
     1141 atgtgctggc ggccactttg ccgctgaacc gttgtttgtt gccgtcgcgc gtggtgatgt
     1201 ccatcacgct gctcatgcgc ccgccgtact cggcgttgaa tccaccggta taaatatccg
     1261 cgttccgcag gatgtcgttg tcgaacacgc tgaaaaggcc gatgctgtgg aaggggttgt
     1321 agagcaccat gccgtccatg agcaccttgt tcatgatggg cgatcccccg cggatgtaca
     1381 gctgcccgcc ctggtcgccg gtgaacacca tgccggggta gacctgcagg gcctgcgcca
     1441 gatcggcctg accgccgatg gccggtaccc gctcgatctc gttgggcgtc atgcgctgca
     1501 cgcttacccg tgtctgtgct tcggcttcct gccggtcgcg tgtcacctcc acatcgggca
     1561 gggcgatggc gcccttcttc aggaagagtt tctccgtggt gatgctcccc gcacgcactt
     1621 ccacctcctt ggacagcgtg tcgtacccga ggtacacgat gcggacggta tatttacctg
     1681 cggggatctt ggtgatgctg aagtagcctt ccacgtcggt ggtggcgtgg aagttggtgc
     1741 ccaccacatt gaccggggtg aagatgctcg gctcgcccgt cgcgtgctcg tacacgaagc
     1801 ctttgagggt gcccgtttgg gcggcggcga agagcgggag gatcagcgag caaaccgctg
     1861 caaagcggta caacttgccg gggatcgggg tgaagggcat caggaaggtg gtacgccgtt
     1921 gcggtggacg ggggtggaaa agggcgccaa aataggcgga tgggccgctt gttcgtccct
     1981 ggaacgacga acggcagcga acggggaggt tccggaccgg gaacttccag ccaccagcgg
     2041 tgccaccctg gtgcggtcgt cttccaaacg gtcagttgtc catccgctcc accatgttca
     2101 cactgggcag gcggcgcagc ttgtccatca atgagcgcaa gtggtcggcg tcgtgtacga
     2161 aggccatcac cttcccctcg aaaacgccgt cgttgctgtc gaaactgatg gaacgcatgt
     2221 tcacgttgtg ctccttgctg atgatgctgg tgatcttgtt cacgatg
//
LOCUS       DAOQOC010000457         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29781_length_2263_cov_3.307518, whole genome shotgun sequence.
ACCESSION   DAOQOC010000457 DAOQOC010000000
VERSION     DAOQOC010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29781_length_2263_cov_3.307518"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PL106_08025"
     CDS             <1..1183
                     /locus_tag="PL106_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33056.1"
                     /translation="INHNNNNQSNASQNYYGEYQVQKKANILGETTITAGLVLNVTNS
                     QAVLYSGDPDHDGHNTARTTAGYLQLDKRFLQERLMVSGGVRYETFKVNQYQRGQPVY
                     RAGATWRALKATYLRASYGQGFRFPTLGERYIRTELDQVRVLPNENLDAEFSVNVEGG
                     IKQGFRIGGFSGYVDVVAFQQDIDHYVEFTFATWAPGTPGNPLGGVGFKSVNTGGARI
                     TGLETEVAGKGNIGPVELTLLMGYTHTKPISTTPDEVYAVTATANPQRLSYAFTSSDT
                     TDHLLKYRMRDLFRADLGVKHKRVSGGVSVRYNSHMRNIDRAFLVLDVSGAMPTGVSR
                     WMQDHTTGTWVVDARVGYQLTPQLKASIIASNISNEVYSIRPMSVEAPRSWQVQLTAE
                     L"
     gene            1180..1932
                     /gene="kdsB"
                     /locus_tag="PL106_08030"
     CDS             1180..1932
                     /gene="kdsB"
                     /locus_tag="PL106_08030"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNA33057.1"
                     /translation="MIRILGVIPARYASSRLPGKPLADIGGRSMVMRVVDQARKCDRL
                     SAVVVATDDERVATHARSYGADVVMTSADHPSGTDRCFEALQHVGAGGFDAVVNIQGD
                     EPFIEPEQIAELCSAIAVPGASIATLAQVVTDDRDLDDAGEVLIVVDKDMNALYFSRA
                     AIPFLRNEVPGRRHARFRYLKHVGLYAYTREALARIVKLVPSSLEQAEQLEQLRWLEN
                     GLPVRVGITTFPSFCVDTPADLEEARRRASAS"
     gene            1943..>2263
                     /locus_tag="PL106_08035"
     CDS             1943..>2263
                     /locus_tag="PL106_08035"
                     /inference="COORDINATES: protein motif:HMM:NF037744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNA33058.1"
                     /translation="MPIERDLHELLYAHDCVIVPRFGGFLTHYRSARIDERQHMVHPP
                     AKDISFNRHLTRSDGLLADHVARAQGLGFAQANAAIEGEVEVWQHKLERDGRLELPRI
                     GTFYR"
BASE COUNT          428 a          705 c          762 g          368 t
ORIGIN      
        1 gatcaaccac aacaacaaca accagtcgaa cgccagccag aactactacg gcgagtacca
       61 ggtgcagaag aaggccaaca tcctggggga aacgaccatc accgccgggc tggtgctcaa
      121 cgtcaccaac agccaggcgg tgctgtacag cggagacccc gaccacgatg gccacaacac
      181 ggcgcgtacc accgcaggct acctgcagct ggacaagcgc ttcctgcagg agcgcctgat
      241 ggtgagcggg ggcgttcgct acgagacctt caaggtgaac cagtaccagc gcgggcagcc
      301 ggtgtatcgg gccggggcca cgtggcgggc cttgaaggcc acctacctgc gtgccagcta
      361 tgggcagggc ttccgattcc ccacactggg cgagcgctac atccgcaccg aactggacca
      421 ggtgagggtg ctgccgaacg agaacctgga cgcggagttc agcgtgaacg tggagggtgg
      481 catcaagcaa ggcttccgga tcggaggctt cagcggctat gtggatgtgg tggcgttcca
      541 gcaggacatc gaccattatg tggagttcac cttcgccacg tgggcgccgg gcacgccggg
      601 caaccccttg gggggcgtgg gcttcaaatc ggtgaacacc ggtggggcgc gcatcaccgg
      661 cctggagacc gaagtggccg gcaagggcaa catcgggccg gtggaactca ccctgctcat
      721 gggctacacg cacaccaagc cgatcagcac cacgcccgat gaggtgtacg cggtgaccgc
      781 cacggccaac ccgcaacgct tgagctacgc gttcaccagt agcgatacca cggatcacct
      841 cctgaagtac cgcatgcgcg acctgttccg tgcggacctg ggggtgaagc acaagcgggt
      901 ctcgggcggc gtgagcgtgc ggtacaacag ccacatgcgc aacatcgacc gggcgttcct
      961 ggtgctggat gtttcggggg ccatgcccac gggggtgtcc cgctggatgc aggaccacac
     1021 cacgggcacc tgggtggtgg atgcgcgcgt gggctaccaa ctgacgccgc agctgaaggc
     1081 ctccatcatc gccagcaaca tcagcaacga ggtgtactcc atccggccca tgtcggtcga
     1141 agcaccgcga agctggcagg tgcagttgac ggcggaacta tgatccgcat cctcggcgtc
     1201 attcccgcgc ggtacgccag cagccgactg ccgggcaaac cgctggcgga catcggtggg
     1261 cgcagcatgg tgatgcgggt ggtggaccag gcccgcaagt gcgatcggct ctccgccgtg
     1321 gtcgtggcca cggacgatga gcgggtggcg acgcatgcgc gatcgtacgg cgccgatgtg
     1381 gtgatgacct ccgcggatca ccccagtgga acggaccgtt gcttcgaggc cctgcaacac
     1441 gtgggggccg gcgggttcga tgccgtggtg aacatccagg gcgacgagcc tttcatcgaa
     1501 ccggaacaga tcgccgagct gtgttccgcc atcgccgttc cgggtgcgtc catcgccacg
     1561 ctggcgcaag tggtcaccga cgatcgggac ctggacgatg cgggtgaggt gctcatcgtg
     1621 gtggacaagg acatgaacgc gctgtatttc agtcgcgcgg ccattccttt cctgcgcaac
     1681 gaggtgcccg ggcggcgcca tgcgcggttc cgttacctga agcatgtagg gctgtacgcg
     1741 tacacccgcg aggcattggc acgcatagtg aagctggtgc cgtcgtcgtt ggagcaggcc
     1801 gaacagctgg agcagttgcg ctggttggag aacggtctgc ccgtgcgcgt ggggatcacc
     1861 acgttcccgt ccttctgtgt ggacacaccc gccgacctgg aggaggcccg caggcgagcg
     1921 agtgcttcgt agtttcgcgg cgatgcccat tgaacgcgac ctgcacgaat tgctgtacgc
     1981 gcacgactgc gtgatcgtcc cgcgtttcgg gggcttcctt acgcactacc gctcggcacg
     2041 catcgatgag cggcagcaca tggtgcatcc gcccgccaag gacatcagtt tcaaccggca
     2101 cctgacccgt tcggacgggt tgttggccga ccatgtggcc cgtgcccaag gattgggctt
     2161 tgcgcaagcg aacgccgcca tcgaaggcga ggtggaggtc tggcagcaca agctggaacg
     2221 cgatgggcgc ctcgagttgc cacgcatcgg caccttctat cgc
//
LOCUS       DAOQOC010000458         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29799_length_2262_cov_3.351155, whole genome shotgun sequence.
ACCESSION   DAOQOC010000458 DAOQOC010000000
VERSION     DAOQOC010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29799_length_2262_cov_3.351155"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="purL"
                     /locus_tag="PL106_08040"
     CDS             complement(<1..1320)
                     /gene="purL"
                     /locus_tag="PL106_08040"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNA33059.1"
                     /translation="MSTTKTIQPDTAPASLETAKKLGLREEEYGKIQEYLGRVPNFTE
                     LSIYSAMWSEHCSYKNSIRLLKTLPRTGPKLLAEAGQENAGLVDIGDGWACAFKIESH
                     NHPSAIEPFQGAATGVGGINRDIFTMGARPIAQLNSLRFGDITTDARARWHMRGVVKG
                     IGSYGNAFGVPVLGGEVCFDPCYSTNPLVNAMSVGIVRTDKVISATSHGTGNPVFIVG
                     SATGKDGIHGASFASKDMTDTSMDDLPAVQVGDPFMEKLLLEASLELAQTDAIIGMQD
                     MGAAGITCSTSEMSAKGESGMDIHLDRVPTRQEGMRAWEILLSESQERMLVVVKKGRE
                     QEVQRIFEKWDLHCVDIGTVTNGNTLRYYMHGELVAEVPADSLVLGGGAPVYVREERE
                     PASFKENQGFRTDQVPEPDDLNAVAYDLLASPNIASKRWVSEQYDTMV"
     gene            1674..>2262
                     /locus_tag="PL106_08045"
     CDS             1674..>2262
                     /locus_tag="PL106_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33060.1"
                     /translation="MTGLLLALAYASLFLYAIRKVPFFSAPGLPRRWIGAFLLLKIVA
                     GTALWYVYTYVHTDRATADIYRFFDDGNIMFRALPEHPLDYLRMITGIGNDQKRFDVN
                     YYQVMNNWYRQYEGNLYNDAHTMIRFNAFVRLFSFGHYHVHTVFICFFCTIGLVALYR
                     AFAPLLPGHERAWSLTLFVLPSVLFWTSGVLKEALL"
BASE COUNT          360 a          746 c          688 g          468 t
ORIGIN      
        1 caccatggta tcgtactgct cgctcaccca gcgcttgctg gcgatgttgg ggcttgccag
       61 caggtcgtag gccacggcgt tcaggtcgtc gggctcgggc acctggtcgg tgcggaagcc
      121 ctggttctcc ttgaacgaag cgggctcgcg ctcctcgcgc acatataccg gcgcgccacc
      181 gcccagtacc aggctgtcgg cgggcacttc ggccaccagc tcgccgtgca tgtagtagcg
      241 cagggtgttc ccgttcgtca cggtgccgat gtccacgcaa tgcagatccc acttttcgaa
      301 gatccgttgc acctcctgct cacggccctt cttcacaacc acgagcatgc gctcctgact
      361 ttcgctcagc aggatctccc acgcacgcat gccctcctgg cgggtgggca cgcggtccaa
      421 gtggatgtcc atgccgctct cccctttcgc gctcatctcg ctggtgctgc acgtgatgcc
      481 ggccgcgccc atgtcctgca tcccgatgat ggcgtcggtc tgcgcgagct caagcgaggc
      541 ttcgagcaat agcttttcca tgaagggatc gcccacttgc acggcgggca gatcgtccat
      601 gctggtgtcc gtcatgtcct tgctggcgaa gctggcgccg tggatgccgt ccttgccggt
      661 ggcgctgccc acgatgaaga cgggattgcc cgtgccgtgg ctcgttgcgc tgatcacctt
      721 gtcggtacgc acgatgccca cgctcatggc gttcaccaac ggattggtag aatagcacgg
      781 atcgaaacag acctcgccgc ccagcacggg cacgccgaac gcgttgccgt agctcccgat
      841 gcccttcacc acgccgcgca tgtgccagcg ggcacgcgca tcggtggtga tgtcgccgaa
      901 gcgcaaactg ttgagctgcg cgatgggccg cgcacccatg gtgaagatgt cgcggttgat
      961 gccgcccacg cccgtggccg caccctggaa gggctcgatg gcactggggt ggttgtggct
     1021 ctcgatcttg aaggcgcacg cccagccatc gccgatgtcc accaggcccg cgttctcctg
     1081 cccggcctcg gccagcagtt tggggccggt gcgcggcagg gtcttcagca ggcggatgga
     1141 gttcttgtac gagcagtgct cactccacat cgcgctgtag atgctcaact cggtgaagtt
     1201 gggtacgcga cccaggtatt cctggatctt cccgtactcc tcttcgcgca ggccgagttt
     1261 cttggcggtc tccaaggagg caggggccgt gtcgggttgg atggtcttgg tcgttgacat
     1321 gcggcgcgaa agtagcccga gcgaggcggt caggcgtggt ggtcgtgcat cagcaggcga
     1381 tcgtcatcaa agaatgggca ggaggccgca gctcccggcc ttgtgaggct cactcctggt
     1441 cgcaagtgtt ccacgcaggt ggccatgcgg aaggggagcg gttcccttgt gaccttccct
     1501 ctcatgaatg ccgcaagtgg ccccgcgtgt tcggcgggat catgtccatg gagggaacac
     1561 gtgcgacatg gagggcaacc accctgtgca gggcttcgcc cggtgcccga gcagatcagc
     1621 gaccgaacgg gtgcaagggt ccggaagacc ccgcccggta cattcgtcgg ctcatgaccg
     1681 gacttttgct cgcccttgcc tacgcgagcc tcttccttta cgccatccgg aaggtgccct
     1741 tcttcagtgc acccggcctg ccgcgccgat ggatcggcgc cttcctcctg ctgaagatcg
     1801 ttgctggaac ggccctgtgg tacgtgtaca cctacgtgca taccgaccgc gccaccgctg
     1861 acatctaccg cttcttcgat gatggcaata tcatgttccg tgccctgccc gagcacccgc
     1921 tggattacct gcgcatgatc accggcatcg gcaacgacca gaagcgcttc gacgtgaact
     1981 actaccaggt gatgaacaac tggtatcgcc agtacgaggg caacctgtac aacgacgcgc
     2041 ataccatgat ccgcttcaat gccttcgtgc gcctgttctc cttcggccac taccatgtgc
     2101 acacggtctt catctgcttc ttctgtacca tcggactggt agcgctctac cgggccttcg
     2161 cccccctgct ccccggtcac gagcgggcct ggtcgctcac gctcttcgtt ctgcccagcg
     2221 tactcttctg gaccagcggc gtgctgaaag aggcgttgtt ga
//
LOCUS       DAOQOC010000459         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_29938_length_2256_cov_3.262154, whole genome shotgun sequence.
ACCESSION   DAOQOC010000459 DAOQOC010000000
VERSION     DAOQOC010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29938_length_2256_cov_3.262154"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PL106_08050"
     CDS             <1..351
                     /locus_tag="PL106_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNA33061.1"
                     /translation="EEHYRCAQRVKAILQRYKELQDIIAILGMDELSEEDKLSVFRAR
                     KVQRFLSQPFFVAEQFTGLPGVMVPIEETIKGFNMILDGQMDEYPEPAFNLKGTIDDV
                     ITAGKKMLAEAAKA"
     gene            441..677
                     /gene="atpC"
                     /locus_tag="PL106_08055"
     CDS             441..677
                     /gene="atpC"
                     /locus_tag="PL106_08055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNA33062.1"
                     /translation="MRLEIITPDKELYKGDAKLVSVPGVDGSMGFLDNHAPLITVLKA
                     GEVKVTLPDGKVEHFPVKGGVVEVMKNTVLVLAE"
     gene            993..1781
                     /locus_tag="PL106_08060"
     CDS             993..1781
                     /locus_tag="PL106_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNA33063.1"
                     /translation="MSSSPDPKKYSTEEAELRRTLVALRTHPPKPMEHPGTRIREILG
                     LMPLFPDHFTYVYDFSEARIRYCRGFPEVLGYKNEEVDLDMLFALVHPDDAPIIWRIN
                     RCVVEAISQLDRPLDPFDICLTVDYRIRKSNGHYIKVLRNTAIFDTDEGTGKVTSTFS
                     LCRDISTFKSSNSIGWQVKGARADQLDTSALDDLTPRMLYRPTTREMEVVRELALGKS
                     TKLIALELKLSEHTVTTHRKNLLKRTGMKNTVELVHHVKEAGWL"
BASE COUNT          498 a          689 c          661 g          408 t
ORIGIN      
        1 gaggagcact accgctgcgc ccagcgtgtg aaggccatcc tccagcgcta caaggaactc
       61 caggacatca tcgcgatcct gggcatggac gaactgagcg aggaggacaa gctgagcgtg
      121 ttccgcgccc gcaaagtgca gcgcttcctc agccagccct tcttcgtggc cgaacagttc
      181 accggcctgc ccggcgtgat ggtgccgatc gaagagacga tcaaaggctt caacatgatc
      241 ctcgacggcc agatggacga gtaccccgaa ccggccttca acctgaaagg caccatcgac
      301 gacgtgatca ccgccggtaa gaagatgctc gctgaggccg cgaaagccta acaagctgcg
      361 agggtgaaag ggtgcgagag cagtgcccga ggttaccctc gccctctcgc actctctcac
      421 gccgggatac ccatcacaac atgagactcg agatcatcac tccggacaag gagctttaca
      481 aaggcgacgc gaagctcgtt tccgtgcccg gtgtggacgg cagcatgggc ttcctggaca
      541 accacgcccc gctgatcacc gtgctgaagg ccggcgaggt gaaggtgacc ctgcccgatg
      601 ggaaggttga gcattttccc gtgaagggtg gcgtggtgga agtgatgaag aacaccgttc
      661 tggtgctcgc cgaataattt ctaccgcact cgtgacccag gtggcggagt aaatctcggt
      721 ctaccgaagt catgaacgac ggccccggca tgttcggggc cgttcgtgtt ggtgggccta
      781 cgacctgggg acataggcgc atggccgtac gaacgtgatg cggatccata acggcatcca
      841 ccacaccatg gtaacgatgc tggaccacca acggggagga cacccgggcg aacgcgtcca
      901 tgatcccggt cttcaggtgc gttcgacccg gggacgggac cgccccttga acaattgtcg
      961 tatactggta ccatcgtcaa tgcccactcg aaatgtcctc ttctcctgat cccaagaaat
     1021 actccaccga agaagccgaa ctgcgccgaa ccctggtcgc actgcgcacc catccaccga
     1081 agccgatgga acacccgggc acgcgcatca gggagatcct gggcctcatg cctcttttcc
     1141 cggatcattt cacctacgtg tacgattttt ccgaagcccg catccggtac tgccgtggct
     1201 ttccggaggt gctcggctat aagaacgaag aggtcgatct cgacatgctc tttgccctgg
     1261 tgcatcccga tgatgcgccg atcatctggc gtatcaacag atgcgtagtg gaggccatct
     1321 cccaattgga tcggcccttg gacccgttcg acatctgcct caccgtggac tatcggatcc
     1381 gcaagtcgaa cggccactac atcaaggtgt tgcggaacac cgcgatcttc gacaccgatg
     1441 aggggaccgg gaaggtgacc agcacgttca gcctgtgcag ggacatcagc accttcaaat
     1501 cgagcaacag catcggctgg caggtgaagg gcgcgcgcgc ggatcaactg gatacgagcg
     1561 ccctggacga cctcacgccc cggatgctgt accggcccac cacacgcgag atggaggtgg
     1621 tccgcgaact ggctttgggc aagagcacca aattgatcgc ccttgaactg aagctgagcg
     1681 aacacacggt gaccacgcac cgcaagaatc tgctgaagcg caccggcatg aaaaataccg
     1741 ttgaactcgt acaccacgtg aaggaagcgg ggtggctgtg aaacggacgg gctccgatga
     1801 tgatgggcca cgaacagatc atggtcgcgc ccaggtccgg ggaacggtgg atgtgttgct
     1861 cgtccgctga aaggacagcg tgatccgcct gaggtgggat caccgtgtgg atcgtatagc
     1921 acccataggc cgagcgcacg atctcctgcg cggaggaagt cctggaactc cgaacggatg
     1981 atccgcctca agtgaccatc atggacctga acctgccggg cctgagcggt atctgatacc
     2041 atctacgcat tcaataccag ccgatccgct tggcctcttt ccggatgcct tctccgcgaa
     2101 acaggttccg taggccccgc tacgcaacct gtttcgcgta tcgtcctccg aaaagatcac
     2161 ggcgcggctc ttggccggcc ttgaatgcgt aaatggcata acgcgctagc gccacaggga
     2221 atgcgtggcg accttaccgg tgccgtgctc cacgat
//
LOCUS       DAOQOC010000460         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30032_length_2252_cov_3.634957, whole genome shotgun sequence.
ACCESSION   DAOQOC010000460 DAOQOC010000000
VERSION     DAOQOC010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30032_length_2252_cov_3.634957"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PL106_08065"
     CDS             <1..608
                     /locus_tag="PL106_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33064.1"
                     /translation="EQPLCFTNAWNLYPDGTRQDYLRGWLGGRVPEGTVGLRDIVARN
                     FIPTASIMHRRAVWEALPEEVRSLSSWDWLLVTAMATRGPLGYLDRITCVRRVHEGGA
                     ISMKPHMDKIGVNLRLLDQVDLISAHRCMDITNARRAELCGMAVQHAVDSGRASAGAP
                     FIRRLVRTAALRNQTGTRDLMRWWILVRAPWLGRWIHRLRR"
     gene            656..1357
                     /locus_tag="PL106_08070"
     CDS             656..1357
                     /locus_tag="PL106_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA33065.1"
                     /translation="MDPYLDLPLNADTQDLYWTRTSIRQALRAQLDRFEGEFLDIGCG
                     LQPYRPLITNAPSRVKRYVGMDLADNLVGTYRAVRPDVEWDGRRIPLPDHSFDSAMAT
                     EVLEHCPDPGAVLREANRVLRPGGSFFMTVPFLWPLHDIPYDEYRYTPFALQRLLNDA
                     GFKEVTVKALGGWDASLAQMLGLWAVRRPMPAWKRKVMKRITLPVIKYLLRHDVVPDP
                     RSSPMITGLWAIAIK"
     gene            1363..>2252
                     /locus_tag="PL106_08075"
     CDS             1363..>2252
                     /locus_tag="PL106_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA33066.1"
                     /translation="MRIALISPNRNAWSETFIANHIAHLPGVALVLMDGHLPKRDIDG
                     EPLLAPTLVKRVVARVRRADPQDMLRATIARRLKKHRIDVVLAEYGPTGTAMVDVCER
                     AGIPLVTHFHGIDAFHTRILKDNADYAQLCRAASAVVVVSREMERQLSGLGCPREKLH
                     YNCYGIEVDRFTPADVARSSKTFLAVGRFVDKKAPHLTLTAFAQAQARDPELRLVMAG
                     DGPLWESTRSLAGALGVADAVEFPGVITQDQVAERMRTARAFVQHSVITRDNDHEGTP
                     LSILEAMASGLPVVSTRHAG"
BASE COUNT          407 a          733 c          741 g          371 t
ORIGIN      
        1 cggagcagcc cctgtgcttc accaacgcat ggaacctgta ccccgatggc acccggcagg
       61 actatctgcg cggctggctc ggcggccgtg tgcccgaagg cacggtgggc ctgcgggaca
      121 tcgtggcgcg caacttcatc cccaccgcca gcatcatgca ccgccgcgcc gtgtgggagg
      181 ccctgcccga ggaggtacgg agcctctcct catgggactg gctgctggtg acggccatgg
      241 ccacgcgcgg gccgctgggc tacctggacc gcatcacgtg cgtgcgccgc gtgcatgaag
      301 gcggcgccat cagcatgaaa ccccacatgg acaagatcgg tgtgaacctg cggctgctgg
      361 accaggtgga cctgatcagc gcccatcgct gcatggacat caccaacgcc aggcgcgccg
      421 aactgtgcgg gatggcggtg cagcacgccg tggacagcgg tcgagcgtcc gcaggcgcgc
      481 ccttcatccg gcgcctggta cgtacagccg ccctgcggaa ccaaacgggt acccgtgacc
      541 tgatgcgctg gtggatcctg gtgcgtgccc cgtggctcgg ccgatggatc caccgtcttc
      601 gccggtgagg accgcacgga tgcccacaag gacagagccc cgaacttcgc cgccaatgga
      661 cccctacctg gatctgccgc tcaatgcgga cacacaggac ctctattgga cgcgcacgtc
      721 gatccgccag gccctgcgcg cgcaactcga ccggttcgaa ggtgaattcc tggacatcgg
      781 ttgcgggctt cagccctacc gaccactgat caccaacgca ccttcacgcg tgaagcgtta
      841 tgtgggcatg gacctggcgg acaatctggt gggcacctac cgggcggtgc ggcccgatgt
      901 ggaatgggac ggcaggcgta tccccctgcc ggatcacagt ttcgacagtg cgatggccac
      961 cgaggtgttg gagcattgcc cggatcccgg ggccgtgctc cgcgaggcga accgcgtatt
     1021 gcggcccggc ggcagcttct tcatgaccgt gcccttcctg tggccgttgc atgatatccc
     1081 atacgacgag tatcgatata cgcccttcgc actccaacgg ttgctcaacg acgcgggctt
     1141 caaggaggtc acggtgaaag cactcggcgg ctgggatgcc agcctggccc agatgcttgg
     1201 cctgtgggcg gtgcgcaggc ccatgcccgc ctggaaacgc aaggtgatga aacggatcac
     1261 cctgccggtg atcaagtacc tgctccggca tgatgtggta ccggacccgc gctcttcgcc
     1321 catgatcacg gggctctggg ccatcgccat caagtgaggc ccatgcgcat cgccttgatc
     1381 agccccaacc gcaacgcgtg gagcgagacc ttcatcgcga accacatcgc gcatctgccc
     1441 ggtgtggcgc tcgtactcat ggacggtcat ctgcccaagc gcgacatcga cggggaaccg
     1501 ctgctggcac caacacttgt gaagcgcgtg gtggcgcgcg tacgccgggc cgatccacag
     1561 gatatgctgc gcgcaaccat cgcacggcga ttgaagaagc accggatcga cgtggtgctg
     1621 gccgaatacg ggcccacggg aacggccatg gtggatgtgt gcgaacgtgc ggggatcccg
     1681 ctggtgacgc actttcacgg catcgatgcc ttccacacac ggatcctgaa ggacaacgcg
     1741 gattatgcgc aactgtgccg ggcagcgagt gccgtggtgg tggtgagccg cgagatggag
     1801 cgccagttat cagggctggg ctgcccccgt gaaaaactgc actacaactg ttacggtatt
     1861 gaagtggacc gtttcacgcc ggccgatgtg gcgcgatcgt cgaagacctt cctggccgtg
     1921 ggccggttcg tggacaagaa agcaccgcac ctcaccctca ccgccttcgc gcaggcgcaa
     1981 gcacgcgacc cggaactgcg cctggtgatg gccggggatg gtcccttgtg ggagagtacg
     2041 cgatcgctgg ccggtgcgct gggggtggcc gatgcggtgg aattccccgg ggtgatcacc
     2101 caggaccagg tggccgaacg catgcgcacc gcacgggcat tcgtgcaaca cagcgtgatc
     2161 acgcgcgaca acgaccacga ggggacgccc ctgagcatcc tggaggccat ggcaagcggc
     2221 ttgcccgtgg tgagcacacg tcatgccggt at
//
LOCUS       DAOQOC010000461         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30135_length_2249_cov_3.945761, whole genome shotgun sequence.
ACCESSION   DAOQOC010000461 DAOQOC010000000
VERSION     DAOQOC010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30135_length_2249_cov_3.945761"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PL106_08080"
     CDS             complement(<1..1155)
                     /locus_tag="PL106_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33067.1"
                     /translation="MAQTPNRLPSRGSQASGPALEGTLREIKQGVDRIAEAMGDGADV
                     DVSSLAKEVTLGQLLTAILGVIAPAGTPAVPSLNVNVVETVGGGGGGGDATAANQVLE
                     ISALNSILAKIIAAPSTEAKQDAIIAALGPLATEVTVANILAALATPALPAGAATEVT
                     LAAILAAMGAPPLPSGAATEATQLLNWGLLGSINSALGSPLTINLPAGISTEAKQDAI
                     ITALGLLSTAANQTTGNTTLASILAALSGTLAVSAASLPLPTGASTAANQTTGNNYLY
                     SIDQWLQALYDAQRQQGQTVGSAQTWLSAARQRATPSDEATEGTVAWLQMSGGRLYTR
                     TILDAAVPAGANVIGKVSIDQTTPGTTNAVEVIQVPALVKGTQGARGLSVQ"
     gene            complement(1157..>2249)
                     /locus_tag="PL106_08085"
     CDS             complement(1157..>2249)
                     /locus_tag="PL106_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33068.1"
                     /translation="IGIAAYGGVPLTCGTITTFGNSIAGFAGLGTALSTINIVTLSGD
                     DNAALLLLDDMGYGAPGGTVHVGLLKSEGGITAGHTFRNQVAGYFRGQYSVNVGVVSF
                     ANKAPSVPEAMFVVDPKLPGGANQRSYLQVGSCKGFGYQYLVSNRVTGAKWTSSGEYS
                     ACGWEHYASGDALYTNATGVTKVTGGGPVDPPGTWTCTAWSACSGGTQTRTCTCSGTC
                     ADPKPAESQTCTVTPEPTVLTFTPLAPLKDGTVEGVLREARFAVDGDQNSFWISDRSM
                     ANGQAVEVSFPSRSVAGVTFSTGRYSNSYPRTYTVDYWTGSAWSRLGSFTGGVNNSPA
                     WGAKTTTKLKITCTTANGNWWAVNEVTLK"
BASE COUNT          370 a          759 c          676 g          444 t
ORIGIN      
        1 ctgtaccgac aagcccctcg ccccttgtgt acccttgacc aaggccggaa cttggatgac
       61 ctcgaccgcg ttggtggtgc cgggcgtggt ctggtcgata gacaccttgc cgatgacgtt
      121 cgccccagca ggtacggcag catcaagaat ggtgcgcgtg tagaggcgtc caccggacat
      181 ctgcaaccat gccaccgttc cctccgtggc ctcatcgctg ggcgttgccc gctggcgagc
      241 ggcagatagc cacgtctgtg cgctgccaac cgtctgcccc tgctgccttt gcgcatcata
      301 cagggcttgc agccactgat ctatgctgta caggtagttg ttgcccgtgg tctggtttgc
      361 cgccgtggac gcacccgtgg gcaacggcaa gctcgctgca ctgaccgcca aggtgcctga
      421 gagggccgca aggatgctgg ccagcgtggt gttcccggtg gtctggtttg ccgccgtgga
      481 caacagaccc aaggccgtta tgatcgcgtc ctgcttggct tccgtgctga tgcccgcagg
      541 caggttgatg gtcagcggag aaccgagcgc cgagttgata cttcccagaa gcccccagtt
      601 gagcagttgc gtggcctcgg tcgctgcacc tgacggaagg ggaggggccc ccatcgcggc
      661 gagtatggcc gccaatgtga cttccgttgc cgcgccagca ggaagggcgg gtgtagcgag
      721 cgccgcgagg atattcgcta cggtgacttc cgttgccagt gggccaaggg cggcaatgat
      781 ggcatcctgc ttggcctcgg tactgggcgc ggcgatgatc ttggcaagga tgctgttcag
      841 ggcagatatc tccagcacct gattggccgc cgtggcatca ccaccgcccc cacctccacc
      901 caccgtctcc acaacattca cgttgagcga cgggacggct ggggtgccag cgggtgctat
      961 taccccaaga atggccgtca gtagttgccc cagcgtcacc tctttggcca gtgaactcac
     1021 gtccacatcc gcgccgtcgc ccatagcctc ggcaatgcgg tcaacgccct gcttgatctc
     1081 gcgcagggtt ccctccaaag cagggcccga tgcctggctg ccgcgcgagg ggaggcggtt
     1141 gggggtctgt gccatactac ttcagtgtca cctcatttac cgcccaccag ttcccgttgg
     1201 ccgtggtgca ggtgatcttg agcttcgtgg tcgttttcgc tccccacgcc gggctgttat
     1261 tcacccctcc agtaaagctc ccgagccggg accacgccga tccggtccag tagtccaccg
     1321 tataggtccg ggggtagctg ttggagtagc gccccgtgct gaaggttacc ccggccaccg
     1381 agcgcgaagg gaaggacacc tctaccgcct gtccgttggc cattgaccgg tcactgatcc
     1441 agaaactgtt ctgatcgccg tccaccgcga acctcgcttc cctcagcact ccttccaccg
     1501 tcccgtcctt cagcggagca aggggcgtga aagtcaacac cgtcggctcc ggcgtcaccg
     1561 tgcaggtctg cgattcggcg ggcttgggat cagcacacgt ccccgagcag gtgcaggtcc
     1621 gggtctgtgt tccgccggaa cacgcgctcc acgccgtgca ggtccatgtc cccggtggat
     1681 cgaccggccc tccccctgtg accttggtaa cgccggtcgc gttggtgtac agggcgtccc
     1741 cgctggcgta gtgctcccac ccgcacgccg aatactcgcc gctgctcgtc cacttcgcgc
     1801 ccgtcacccg attgctcacg aggtattggt agccgaagcc cttgcaactc cccacctgaa
     1861 ggtacgatcg ctggttcgcc cctcccggca gcttgggatc aacaacgaac atcgcctccg
     1921 gcacgctcgg ggccttgttc gcgaagctca ccacgcccac gttcacgctg tactggccac
     1981 ggaaataccc ggctacctga ttgcggaatg tgtgacctgc tgtgatcccg ccttcacttt
     2041 taagtaaacc cacgtgcacc gtccctcctg gcgctccgta ccccatgtca tccaagagca
     2101 gcagcgccgc attgtcatcc ccggaaaggg tgacgatgtt gatcgtggac agggcggtcc
     2161 ccagtcctgc gaaccccgcg atggaattgc cgaacgtggt gatcgtcccg cacgtcaggg
     2221 gcacgccacc gtatgccgcg atgccgatg
//
LOCUS       DAOQOC010000462         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30182_length_2247_cov_4.039690, whole genome shotgun sequence.
ACCESSION   DAOQOC010000462 DAOQOC010000000
VERSION     DAOQOC010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30182_length_2247_cov_4.039690"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1531
                     /gene="carB"
                     /locus_tag="PL106_08090"
                     /pseudo
     CDS             1..1531
                     /gene="carB"
                     /locus_tag="PL106_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            1729..2022
                     /locus_tag="PL106_08095"
                     /pseudo
     CDS             1729..2022
                     /locus_tag="PL106_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
BASE COUNT          519 a          663 c          725 g          340 t
ORIGIN      
        1 gtgcgagaag ctcggcatct ggaaacagta cggcgtgcgc atgatcggcg tggacgtgaa
       61 ggcgatcgac atcaccgaga accgcgagca gttccggcgg ctgatggaga agatcggcgt
      121 gcccatggcg cagagcaagg tggtgaacag cttcctggaa gggaagaagg tggcgcagga
      181 gttcggcttt ccgctggtga tccgcgccag ctacacgctc ggtggcggcg gcgccagctt
      241 cgtacacaag gaggaggatt tcgacaagct gctgaagcac ggcctgcagg tgagccccat
      301 ccacgaggtg atcattgaca aggcgctgct gggctggaag gaattcgaac tggaactgct
      361 gcgcgacaag gacgacaacg tcgccatcat ctgcagcatc gagaacttcg atccgatggg
      421 catccacacc ggggacagca tcaccgtggc gccggcgatg accctcagcg accgcactta
      481 tcagaagatg cgcaccgagg ccatcaagat gatgcgctcc atcggcgact tcgccggggg
      541 gtgcaacgtg cagttcgccg tgagccccga tgaaaaggag gagatctacg ccatcgagat
      601 caatccgcgc gtgagccgca gcagcgcgct cgcgagcaag gcgacaggtt atcccatcgc
      661 caagatcgcc agcaagctcg ccatcggtta ccgcctggat gaactggaga atcccatcac
      721 agggaccagc gccttcttcg agcccacgct cgattacgtg atcgtgaaag tgccgcgctg
      781 gaacttcgac aagttcgagg gcagcgaccg tcgcttgggt gtacagatga agagcgtcgg
      841 cgaaacgatg ggcatcggac gcagtttcca ggaagcactg cagaaagcct gtcagagcct
      901 cgagatcaag cggaacggcc tgggcgccga tggcaaggaa acgcgcgatg ccgccgtcct
      961 gctggacagc ctcgccaacc cgagctggag ccgcctcttc cacgtgtacg atgcgatcaa
     1021 actgggcatc cccttcagca agatccacga gctcaccaag atcgacatct ggttcctgaa
     1081 gcagatcgag gacatgatcc tcaccgagaa ggaggtggag aaatacacgc tcgacaccat
     1141 cccgcgcgac ctgctcttcg aagcgaaaca gaagggctac gccgatcgac aggtcgcgca
     1201 cttgctcggc tgcctggaga gccaagtgta caagaagcgc gaggagctcg gcatcaaacg
     1261 cgtgtacaag ttggtggaca cctgcgccgg tgaattccct gcgaagacgc cctactacta
     1321 cagcaccttc gaggaggaga acgagagcgt acgcagtgac aagaagaaga tcgtggtgct
     1381 cggcagcggc cccaaccgca tcggccaggg catcgagttc gattacagct gcgtgcacgg
     1441 cgtgctcgcg gccaaggagc tcggctacga gaccatcatg atcaactgca acccggagac
     1501 ggtgagcacc gacttcgaca cggcggacaa gcaaggctgc ctcgaaggac ctcgaacgga
     1561 tggcacgcgt ggctatcgtt cggccagacg aggcgcgacg aagggaggct acccataggt
     1621 agctgaccaa ggaagcaacg aagtatgggc gaatgagagc cgtgcgcccg aagggacaac
     1681 gcgaagcgtt gatgccgccg gaacgatgtc cttcgtggta gcctaaactt cgagcccgtg
     1741 ttctgggagc acatctacga catcatccag cacgaacagc ccgagggcgt catcgtgcag
     1801 ctcggtggac agaccgcgct gaagctcgcc gagaagctgg agaagtacgg catcaagatc
     1861 atcggcacca gctacgccgc gctggacatg gccgaggacc gcgagcgttt ctcctcgctg
     1921 ctgcgcgacc tcaacatccc gtaccccaag ttcggcgcgg tacacaacgc cgaggaagcc
     1981 gtgaagctca gccgcgaact cggcttcccg ctgctggtgc ggcttggtgt ggcaaccctt
     2041 gggctggccg atcacgggag cgcgaggcat ttcgccttcc ggcaaggcgg aacccgcgcg
     2101 gcgtagcagg cgctacgacg aagggtgaca acgcggccgg aaggtgaaag gcccgcgcgg
     2161 atgatcggct ggcccatggg ttgccacacg cactacgtgc tcggcggcca gaagatgaag
     2221 atcgtgatca acgaggagga gctcgtg
//
LOCUS       DAOQOC010000463         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30218_length_2246_cov_3.257873, whole genome shotgun sequence.
ACCESSION   DAOQOC010000463 DAOQOC010000000
VERSION     DAOQOC010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30218_length_2246_cov_3.257873"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2246)
                     /locus_tag="PL106_08100"
     CDS             complement(<1..>2246)
                     /locus_tag="PL106_08100"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33069.1"
                     /translation="ITTVSTDNLYPHWNLNAACGTQNSCYTTTVQNLGSASACSGTPA
                     PGNTTGPVQATLGSNITLALQNQTTGTGVTYQWYEGPSASGPWTPTGASAATLNTTFT
                     GATWYYCDVTCSGNTGSSAPWGVALDPSIQVGSGSGTYNYFPIYTFYGYNYSQQIYTA
                     SEIAAAGGGPGTITTLRFYMSNNAGFGNWTDWVVYLGNTTQEALATTSSWIPVSSLTQ
                     VFNSTIANPANGTWLELTLSTPFVYDGTNLVVAVDENTPNYNSTSAWGSFTAAPPGSG
                     FRGLLYYSDGTNPNPAGPPTANKSNTTTRAQIQLAITPLPACTTPVPGNTVASNTLPC
                     PGANVSLSMDGSNIYGDGMTYQWQRSADIAFTTPTNLGTLASQVATQGPGETWYYRCV
                     VSCSGTDVASTPVEVVSNTDACFCGTYCPTISYGSYACISAVSINTLSSTTTGCPGTV
                     VLKPETTTLQKNVQYPLTVSIAAPYTAIASVWFDWNNDLNFTADEWTQVYTNASSGSV
                     NITVPNGAYEGTIRMRVRSAGVGSANNGSAGCNTNFFSGTSEDYCITIEPQPACSGTP
                     APGNTTGPISALDGNTISLGLQNLVGSGTTFQWYVSNVSATGPWTPVGPNAPNFSATL
                     AGDSWYYCDVTCTNSAETTASGVLFVDALSVFVVPVSGSNTNPCGNNVVLVDNGGPSG
                     LYANNSDGYTVLEAGIGATITINGPYVGEGCCDHLYIYDGAGTGGTILLDQVGSTTIN
                     YTGTPGQTL"
BASE COUNT          385 a          616 c          766 g          479 t
ORIGIN      
        1 agcgtctgac ccggggtgcc ggtgtagttg atggtcgtgc tgcccacttg gtccagcaat
       61 atcgtgccac cggtacctgc accgtcgtag atgtagaggt ggtcgcagca gccctcgccc
      121 acgtatgggc cgttgatcgt gatggtggca ccgataccgg cttccagcac ggtgtaccca
      181 tcagagttgt tggcgtataa gccggaaggt ccaccgttgt ccaccagtac gacattgttg
      241 ccgcagggat tggtgttgga gcccgagacc ggaaccacga ataccgagag cgcatccacg
      301 aagagcacac cgctggccgt ggtctcagcc gagttggtgc aggtcacatc gcaatagtac
      361 cagctgtcac cggcgagggt ggcgctgaag ttcggggcgt tcgggcccac cggtgtccat
      421 gggccggtgg ccgatacgtt gctcacgtac cactggaatg tagtacccga gccgacgagg
      481 ttctgcagcc ccaggctgat cgtgttgcca tcgagggcgc tgatcggtcc tgtggtattg
      541 ccgggggccg gcgtcccgct gcaagcgggc tgcggctcga tcgtaatgca atagtcctcg
      601 ctggtaccgc tgaagaagtt ggtgttgcaa ccggcagagc cgttgttggc actgcccacc
      661 ccggcgctgc gtacccgcat gcggatggtg ccctcgtagg ccccgttcgg taccgtgatg
      721 ttcacgctgc ccgatgaggc gttggtgtac acttgggtcc actcatcggc ggtgaagttc
      781 aggtcgttgt tccaatcgaa ccagaccgaa gcgatggccg tgtagggcgc agcaatactc
      841 acggttagcg ggtactggac gttcttctgc agggtggtgg tctccggctt cagtaccacg
      901 gttccggggc atccggtggt ggtgctgctc agggtgttga tgctcaccgc gctgatgcac
      961 gcgtagctgc catagctgat ggtggggcag tatgtaccgc agaagcaggc gtcggtgttg
     1021 gacaccactt ccaccggcgt ggaggccacg tcagttccac tgcacgaaac aacacagcgg
     1081 tagtaccacg tttcgcccgg gccttgggtg gccacctggc tggccagggt acccaagttg
     1141 gtcggggtgg taaaggcgat atcggccgaa cgctgccatt ggtaggtcat accatcgcca
     1201 tagatgttgc tgccgtccat cgaaaggctc acgttggcac cggggcaggg cagcgtgttg
     1261 ctggccactg tattgcccgg tacaggcgtg gtgcaagcgg gcaggggggt gatcgccagt
     1321 tggatctgtg cccgggttgt tgtgttgctc ttgttggccg tgggcggtcc tgcgggattc
     1381 gggttggtac cgtcgctata gtacaacaag ccccggaagc cactgcccgg gggcgcggcc
     1441 gtgaagctgc cccatgccga agtgctattg tagttgggcg tgttctcatc cacagccacc
     1501 acgaggttgg tcccgtcgta tacgaagggg gtggagaggg tcaactcgag ccacgtgccg
     1561 ttcgcggggt tggcaatggt actgttgaat acctgcgtga gggagcttac agggatccaa
     1621 gagctggtgg tggcaagggc ttcttgggtg gtattgccca agtacacgac ccaatcggtc
     1681 cagttgccga agccagcatt gttcgacatg tagaaacgca gcgtggtgat ggtgccgggg
     1741 cctccgcccg cagcagcgat ctcgctggcg gtgtagatct gctggctgta gttgtaaccg
     1801 tagaaggtgt agatggggaa gtagttgtac gtgccgcttc ccgaaccgac ctgaatgctg
     1861 ggatccagcg ctacgcccca tggggcactg ctgcccgtgt tgcccgagca ggtcacatca
     1921 caataatacc aagtggctcc tgtgaaggtg gtattcagtg tggcggcact ggctcccgtg
     1981 ggggtccaag gaccggacgc gctgggaccc tcgtaccact ggtaggtgac tccggtacca
     2041 gtggtctggt tctgcagcgc caaggtgatg ttggacccca acgttgcctg cacgggtccg
     2101 gtggtattgc ccggcgcggg tgtgccgctg catgccgaag ccgaacccag gttctgcacc
     2161 gttgtcgtgt aacaactgtt ctgggtgccg caggcggcat tcaggttcca gtgcgggtac
     2221 aggttgtccg ttgaaacggt ggtgat
//
LOCUS       DAOQOC010000464         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30251_length_2245_cov_3.299087, whole genome shotgun sequence.
ACCESSION   DAOQOC010000464 DAOQOC010000000
VERSION     DAOQOC010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30251_length_2245_cov_3.299087"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PL106_08105"
     CDS             complement(<1..1317)
                     /locus_tag="PL106_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNA33070.1"
                     /translation="MYRSIPPLLIALTTSLAAQVPARYSRVSIALTGPPRDLAGLAAL
                     GLPIDHAEHNGRSCTVEINEADIELVRANGYQVDVLVDDVARWYRERNQGAAPTDERA
                     LGWLCDGPTTFTVPLNFNTGSMGGFLTWDEMQTELDDMAALYPGLISAKAPIGLSVEG
                     RPIHFVRISNDPDTDQDKPEVLYTALHHAREPVSMQQLIHYMWWLLENYGTDPQATYL
                     VDNRELYFVPCLNPDGYVYNQITNPDGGGMWRKNRKVNENGSFGVDLNRNYGHEWGLD
                     DEGSSPNPNSETYRGTAAFSEPETQAVRAFCNAHEFRAALNYHAWGDMIIHPWATQQE
                     LLTPDSAAFYAHAARITRNNGHSTGTCFQVLNYLTNGSSDDWMYGEQDEKPKILAMTP
                     ELGDVDDYFWPPGWRIVPICQNNMDQNLLEAHLVGAYAAATDRSYPV"
     gene            1457..>2245
                     /locus_tag="PL106_08110"
     CDS             1457..>2245
                     /locus_tag="PL106_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HNA33071.1"
                     /translation="MERFIGLIGIVLILGIAFLASNNRKAINKRLVISGLLLQTAIAL
                     LVMKVAPITAFFQWLGKGMEKIEHYSRQGASFVYGGIIAQQPDGSMGNYWGGGFVFAF
                     NITATIILVCVLVAILYHIGFMQRVVAVIAKGMNFIMRVSGAEALSNVASAFVGQVEA
                     QVMIRPYLAGMTKSELLASMSGSLACIAGGILIVYANMGAAAGMDLAPKLITASLMAA
                     PGALVISKIVWPETEKSQTMGTVRLEVKSTYTNVIDAIGHGAGDG"
BASE COUNT          406 a          649 c          743 g          447 t
ORIGIN      
        1 cacgggatac gagcggtccg tggcagcggc gtaggcgccc accaggtgtg cttccaagag
       61 gttctggtcc atgttgttct ggcagatcgg cacgatgcgc cagccgggcg gccagaagta
      121 gtcgtccacg tcgcccagtt caggcgtcat ggccaggatc ttcggcttct cgtcctgctc
      181 gccgtacatc cagtcatccg atgatccgtt ggtcaggtag ttcaatacct ggaaacaagt
      241 tccggtggag tggccgttgt ttcgggtgat ccgggcggca tgtgcataaa aggcggccga
      301 gtcgggcgtg agcagctctt gctgggtggc ccacggatga atgatcatgt cgccccaggc
      361 gtggtagttg agcgcggcac ggaactcgtg ggcattgcag aacgcccgca ctgcctgggt
      421 ttccggctcg ctgaacgccg ctgtgccccg gtacgtctcg ctgttcgggt tgggcgagga
      481 gccctcgtcg tccagccccc actcgtggcc gtagttccgg ttgaggtcca ccccgaagct
      541 cccgttctca ttcaccttgc ggttctttcg ccacatgccc cctccgtccg ggttggtgat
      601 ctggttgtac acgtaaccgt caggattcag gcagggtacg aagtagagct cgcggttgtc
      661 caccaggtag gtggcctggg gatccgttcc gtagttctcc aacagccacc acatgtagtg
      721 gatcagttgc tgcatggata ccggttcacg ggcatgatgc agcgcggtgt acagcacctc
      781 gggcttgtcc tggtcggtat ccggatcgtt gctgatccgc acgaaatgga tgggtcggcc
      841 ctccacggaa agaccgatcg gggctttggc gctgatgagt cccggataga gcgcggccat
      901 atcgtccaac tcggtctgca tttcatccca agtgaggaaa ccgcccatgg agccggtgtt
      961 gaagttcagc ggtaccgtga aggtcgtcgg gccgtcgcac agccagccga gcgcgcgctc
     1021 gtcggtgggc gctgcaccct ggttgcgttc gcggtaccat cgggctacat cgtccaccag
     1081 cacatccacc tgatatccgt tggcgcgcac cagttcgatg tccgcctcgt tgatctccac
     1141 ggtgcagctg cggccattgt gctccgcatg gtcgatcgga aggccaagtg cggccaggcc
     1201 ggccaggtcg cgcggtggtc cggtcaatgc gatggagacg cgggagtacc gggcggggac
     1261 ctgagcggcg agggaggtgg tgagcgcgat gagcagcggc gggatcgaac ggtacatcag
     1321 cagactggat aggagcggaa atgtacacat cgagcaaagg agccgcgtgc ttcgacgggg
     1381 atccgttgaa tgaacaccca cgggggatcc gctatcgggc ggaagtgtac tttgaccgcg
     1441 ccatatacac ccctgcatgg agcgtttcat cggcctgatc ggtatcgtgc tgatccttgg
     1501 tatcgctttc ctggccagca acaaccgcaa ggcgatcaac aagcggctcg tgatcagcgg
     1561 cctgctgctg cagacggcca ttgcgctgct ggtgatgaaa gtggcgccca tcaccgcgtt
     1621 cttccaatgg ctgggcaagg gcatggagaa gatcgagcat tattcccggc agggcgcctc
     1681 gttcgtgtac ggcggcatca tcgcccaaca acccgatggt agcatgggga attactgggg
     1741 cgggggcttc gtgttcgcct tcaacatcac ggctaccatc atcctggtct gcgtactggt
     1801 ggccatcctg taccacatcg gcttcatgca gcgcgtggtg gccgtgatcg ccaagggcat
     1861 gaatttcatc atgcgcgtga gcggcgcgga agcgttgagc aacgtggcca gcgccttcgt
     1921 ggggcaggtg gaggcgcaag tgatgatccg tccgtacctg gctggcatga cgaaaagcga
     1981 actgctggcc agcatgagcg gcagtctggc atgtatcgcg ggcggcatcc tcatcgtgta
     2041 cgccaacatg ggtgccgccg caggcatgga cctggcgccg aaattgatca ccgccagctt
     2101 gatggccgcg cccggagcac tggtgatcag caagatcgta tggcccgaga ccgagaagag
     2161 ccagaccatg ggcaccgtgc gcctggaagt gaagagcacc tataccaacg tgatcgatgc
     2221 gatcggacat ggcgcgggcg atggt
//
LOCUS       DAOQOC010000465         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30271_length_2244_cov_3.058474, whole genome shotgun sequence.
ACCESSION   DAOQOC010000465 DAOQOC010000000
VERSION     DAOQOC010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30271_length_2244_cov_3.058474"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PL106_08115"
     CDS             <1..730
                     /locus_tag="PL106_08115"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNA33072.1"
                     /translation="YLFSRPLPIEDLALLLPEARIARMDQDSTRGRHAFERILTRFGE
                     GDIDILVGTQMVTKGLDFDTVTVVGIVNADQMLRFPDFRAHERAFQMMAQVAGRSGRK
                     RDPGTVYIQGRDIHHPIIGLVTAHNVKGMYDRELEHRRGHGYPPFTRLVRLTLKHRSE
                     DRVVATAGSLGAVLRSRLGDRILGPEAPSVARIRDMHLRAILVKLDRNAYRTGKEILR
                     EAIDQVFARPEHRAIRLVIDVDPM"
     gene            complement(758..1435)
                     /locus_tag="PL106_08120"
     CDS             complement(758..1435)
                     /locus_tag="PL106_08120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNA33073.1"
                     /translation="MEVLFSDRYLIAVRKPGGLPAQADPTGDPHLLRLLAPAFPHDHA
                     LSLPHRLDRPVSGIILVTRTKEALAAMSVLFQRRAVAKTYWSIVSGQPPESGVWEHAL
                     IHDTRNNKARVANDTSGPVARTSFRILSRGDRYALVELVPEGGRFHQLRAQCAAAGFP
                     IKGDVKYGARRGEKDRTIALHARSLAFRHPFTGEALHLEADPPEGGLWRALSGGGQRD
                     VDPEMPV"
     gene            complement(1441..>2244)
                     /gene="panB"
                     /locus_tag="PL106_08125"
     CDS             complement(1441..>2244)
                     /gene="panB"
                     /locus_tag="PL106_08125"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNA33074.1"
                     /translation="TEIKRVTMHTLQEMKQQGLPIAMLTAYDYSLARLVDAAGVDVIL
                     VGDSASNVMAGHETTLPITLDQMIYHASSVVRGVARALVVVDMPFGTYQGNSKEALAS
                     AIRIMKESGGHAVKMEGGEEVLDGVGRILTAGIPVMGHLGLTPQSIYKFGTYVVRAKE
                     EEEAARLKRDALLLEEAGCFALVLEKIPATLAEEVAKSVRIPIIGIGAGSGVDGQVLV
                     LHDMLGINQEFNPRFLRRYADLHGTITGAVEGYVRDVRSRDFPNAKEQY"
BASE COUNT          379 a          760 c          714 g          391 t
ORIGIN      
        1 ttatctgttc agccggcctt tgccgatcga agacctggcg ttgttgctgc ccgaggcccg
       61 catcgcgcgc atggaccagg acagcacacg gggcaggcac gccttcgaac gcatcctcac
      121 gcgtttcggg gagggcgaca tcgacattct cgtcggcacg cagatggtga ccaagggcct
      181 cgacttcgac acggtgaccg tggtgggcat cgtgaacgcc gaccagatgc tgcgcttccc
      241 cgacttccgg gcgcacgaac gcgcattcca gatgatggcg caggtggcgg gtcgcagcgg
      301 tcggaagcgg gatccgggca ccgtgtacat ccagggccgc gacatccatc atcccatcat
      361 cggactggtc accgcgcaca acgtcaaggg catgtatgat cgcgaactgg agcaccgacg
      421 cgggcacggc tacccaccct tcacccgctt ggtcaggctc acgttgaagc acaggtccga
      481 agaccgtgtg gtcgccactg cgggatcact gggagcggtc cttcgttccc gtttgggcga
      541 ccgcatactc ggccccgagg caccctccgt ggcacgcatt cgggacatgc acttgcgggc
      601 gatcctggtg aaactcgacc ggaacgcgta ccgcacgggc aaggagatcc tgcgcgaggc
      661 catcgaccag gtcttcgccc gaccggagca tcgtgccatc cgcctggtca tcgatgtgga
      721 tccgatgtga tcgggcagcg gcgggccggg aacacgatca taccggcatt tcaggatcga
      781 catcccgctg cccaccaccc gacaaggccc gccacaagcc accctccggg gggtccgctt
      841 ccagatgcag ggcttcaccg gtgaacggat gccggaacgc caacgaccgc gcgtgcagcg
      901 cgatggtgcg gtccttctcc cctctgcgcg ccccgtactt cacatcgccc ttgatcggga
      961 atccggccgc ggcgcattgc gcgcgcaact gatggaagcg tcccccttcc ggcacgagtt
     1021 ccaccagggc gtagcgatcg cccctggaca ggatgcggaa ggaggtccgc gccacgggac
     1081 ccgaggtgtc gttcgccacc cgggccttgt tattccgcgt atcgtggatc aatgcatgct
     1141 cccagacccc gctctcgggc ggttgcccgg acacgatgga ccagtatgtt tttgcaacgg
     1201 cacggcgctg gaagagaacg ctcatggcgg cgagggcctc ctttgtgcgg gtgacgagga
     1261 tgataccgct gacggggcgg tccagccggt gcggcaggct cagcgcgtga tcgtgcggga
     1321 atgcgggggc gagcaaccgg agcagatgcg ggtcgcccgt ggggtccgcc tgtgcgggca
     1381 ggccaccggg cttgcgcacc gcgatcaggt accggtcgct gaagaggacc tccacggaac
     1441 tcagtactgt tccttcgcgt tcgggaagtc gcgcgaacgc acatcgcgga cgtagccctc
     1501 cacggccccg gtgatcgtgc cgtgcagatc ggcgtagcga cgcaggaacc gcgggttgaa
     1561 ctcctggttg atgccgagca tgtcgtgcag caccagcacc tgtccgtcca caccgctccc
     1621 cgcgccgatc ccgatgatgg ggatacgcac gctcttcgcc acctcctcgg cgagcgtcgc
     1681 cggtattttt tcaagtacca gggcgaaaca gcccgcttcc tccagcagca gggcatcgcg
     1741 tttcaggcgg gcggcctcct cctcctcctt ggcgcgcacc acgtacgttc cgaacttgta
     1801 gatgctctgt ggtgtgaggc ccaggtgccc catcacggga atgcccgctg taaggatgcg
     1861 accgacaccg tcgagcacct cctccccgcc ctccatcttc acggcgtgtc caccgctctc
     1921 cttcatgatg cggatggccg aggccagggc ctccttcgaa ttgccctggt aggtgccgaa
     1981 gggcatgtcc accaccacca gcgcacgcgc cacgccgcgc accacgctgc tggcatggta
     2041 gatcatctgg tccagggtga tgggcagggt ggtctcgtgc ccggccatca cattgctggc
     2101 actgtcgccc accaggatca cgtccacccc ggcggcatcc accaggcgtg ccagcgaata
     2161 gtcgtacgcg gtgagcatgg cgatgggcag cccctgctgc ttcatttcct gcagggtgtg
     2221 catggtcacg cgtttgatct cggt
//
LOCUS       DAOQOC010000466         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30273_length_2244_cov_3.022841, whole genome shotgun sequence.
ACCESSION   DAOQOC010000466 DAOQOC010000000
VERSION     DAOQOC010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30273_length_2244_cov_3.022841"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /gene="recJ"
                     /locus_tag="PL106_08130"
     CDS             complement(<1..1509)
                     /gene="recJ"
                     /locus_tag="PL106_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNA33075.1"
                     /translation="MSTALERYWRLKPAQPDAVVRALTSDRCSPELAHLLAQRGITTP
                     EEAGIFFRPRLEHLHDPFLMKDMDRAVERIELALGDKERIMVYGDYDVDGTTSVALMY
                     GFLNRFTSNLRHYVPDRYAEGYGISTLGIDRAAADGVSLIIALDCGIKSIDKAAYARE
                     KGIDLIICDHHLPGEKIPEAVAVLDPKRSDCTYPDKELSGCGIGFKLVQALAQHNNMP
                     FVELEELLDLVAISIACDIVPLDGENRTLAYHGLRRLNNGPVRPGLDVMLRVTNNRRP
                     ITVSDLVFTIGPRINAAGRIEHGQQAVELLLSSDPGLAEQIGLRIDENNSKRQGLDKE
                     ITRQALDLFNTEDALRDAWSTVVFNKDWHKGVVGIVASRLIETHYRPTVVLTESNGKA
                     SGSARSVRGFDVHEAISACADLLDQYGGHMYAAGLSMPVENVDAFRERFEHVVRERMP
                     PELRVPVEEIDLELSLDRITDRFVRDIGHMAPFGPRNMRPVFMSCGITTKEAR"
     gene            complement(1565..>2244)
                     /locus_tag="PL106_08135"
     CDS             complement(1565..>2244)
                     /locus_tag="PL106_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNA33076.1"
                     /translation="DAAGKDSAIDHVLSGVNPQGCAVTSFKQPGREELAHDFLWRHAK
                     ATPARGMIGVHNRSHYEEVVVVKVHPEYLIGQRLPDIASPKDADEAFWERRYAAIRAW
                     EEHLVHSGTTIMKFFLHMGKAEQKKRFLERIDEPRKNWKFSIGDVQERAHFDGYMKAY
                     EEAIRATAALHAPWFVVPADEQWESRAIVGRLVRERLERMSLEWPVLTAEEKADLAKA
                     ERLLKAE"
BASE COUNT          351 a          724 c          704 g          465 t
ORIGIN      
        1 acgtgcttcc ttggttgtga tcccacagga catgaacacg ggtcgcatgt tgcgcgggcc
       61 gaagggtgcc atgtgaccga tgtccctcac gaaccggtcc gttatccggt ccagggaaag
      121 ctcgaggtcg atctcttcca cgggcacgcg cagttcgggc ggcatgcgct cgcgcaccac
      181 gtgctcgaaa cgttcgcgga aggcatccac attctccacg ggcatgctca gcccggcagc
      241 gtacatgtgc ccaccgtact ggtccaacag gtcggcgcag gcggagatgg cctcatgcac
      301 gtcgaatcct cgtacactgc gggcactgcc gctggccttg ccattgcttt ccgtaaggac
      361 caccgtaggg cggtagtgcg tctcgatcag ccgcgaggcc acgatgccca ccacgccctt
      421 gtgccagtcc ttgttgaata cgaccgttga ccaggcatca cgcagcgcgt cctcggtgtt
      481 gaacaggtcg agcgcctgcc gggtgatctc cttgtcgagg ccctgcctct tgctgttgtt
      541 ctcgtcaatg cgcaggccga tctgttccgc cagacccggg tcggagctca ggagcagttc
      601 cacggcctgt tgcccgtgct cgatgcgccc cgccgcgttg atacgtggac cgatggtgaa
      661 gaccaggtcg ctcacggtga tgggccgcct gttgttggtc acgcgcagca tcacatccaa
      721 accgggccgc acggggccgt tgttcaaccg gcgcaggccg tggtacgcca gcgtgcggtt
      781 ctcaccgtcc agcggtacga tatcacaggc gatgctgatg gccacgagat caagcagttc
      841 ctccagctcc acgaagggca tgttgttgtg ctgagcgagg gcctgcacga gcttgaagcc
      901 gataccgcat ccgctgagtt ccttgtccgg gtaggtgcaa tcgctgcgtt tggggtccag
      961 cacggcgacc gcctcaggta ttttttctcc gggcaggtgg tggtcgcaga tgatcaggtc
     1021 gatgcccttc tccctggcgt aggccgcctt gtcgatgctt ttgatcccgc aatccagggc
     1081 gatgatgagc gaaacgccgt ccgctgccgc gcgatcgatg ccgagggtac tgatcccgta
     1141 gccctcggcg tagcggtccg gcacgtaatg gcgcaggttg ctggtgaagc ggttgaggaa
     1201 accgtacatc agggccacac tggtggtgcc gtccacatcg tagtctccgt acaccatgat
     1261 gcgctccttg tcgcccagcg cgagctcgat ccgttccacc gcccggtcca tgtccttcat
     1321 caggaagggg tcgtgcaagt gttcgagccg cgggcggaag aaaataccgg cctcctcggg
     1381 cgtggttatg ccgcgctggg cgagcaggtg ggccagttcg ggcgagcaac ggtcgctcgt
     1441 gagggcgcgc acgaccgcgt cgggctgggc tggcttcagc cgccagtatc gctccaaggc
     1501 cgtgctcatg tgccgaaggt agcgaacccg ggccttgtcg cggctattcc ggatggatcg
     1561 ggacctattc cgccttcagg agccgttccg ccttggcgag gtccgctttc tcctcggccg
     1621 tcagtaccgg ccattccaag ctcatccgct ccaaccgttc gcgtaccagt cggcccacga
     1681 tcgcgcgcga ttcccactgc tcatcggccg gcaccacgaa ccagggggcg tgcagggccg
     1741 ccgtggcccg gatggcctcc tcgtaggcct tcatgtatcc gtcgaagtgc gcgcgttcct
     1801 gcacgtcacc gatgctgaac ttccagttct tcctgggttc gtcgatgcgt tcgaggaacc
     1861 gttttttctg ttccgccttc cccatgtgca ggaagaactt catgatcgtg gtgccgctgt
     1921 gtaccagatg ttcctcccag gcccggatgg cggcgtagcg tcgctcccag aaggcttcat
     1981 ccgcatcctt cggcgaggcg atgtccggca gccgttgtcc gatcaggtat tccgggtgga
     2041 ccttcaccac gaccacttcc tcgtaatggc tgcggttgtg tacgccgatc atcccgcgtg
     2101 cgggcgtggc cttggcgtgg cgccacagga agtcgtgggc cagttcctcc cttccgggct
     2161 gcttgaagct ggtgacggca cagccctggg gattcacccc gctgagcacg tgatcgatcg
     2221 cggaatcctt gcccgcagcg tcca
//
LOCUS       DAOQOC010000467         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30497_length_2236_cov_2.691426, whole genome shotgun sequence.
ACCESSION   DAOQOC010000467 DAOQOC010000000
VERSION     DAOQOC010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30497_length_2236_cov_2.691426"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2236
                     /locus_tag="PL106_08140"
     CDS             <1..>2236
                     /locus_tag="PL106_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33077.1"
                     /translation="NGLYYGVTEVGGTGGVGVLFSFNPSDGTYTVLHNFNSSTTGAQP
                     LRGPVIANNGRLYGTCSSGGANNVGTLWEYNIGTSTLTKKFDFDGLTTGKGSTPRGRL
                     MVHSNGTIYGTTQLGGLNGAGCIFSYTAGGVSNTKVYNFPALPAITTGNRPLTGLAQS
                     GSLLYGFTANGGANGHGSIFSFNTSGNVFTNLYNFLATGAGRAPLSEFTAVSGVLYAT
                     ASAGGSSSGGTIFSWDISGAAHADLVHLNNSIGYAPFSRLFAASDGQLYGTTSAGGTS
                     NAGVLFSFNTGTNTYTPVLQMASLGLSAPWSSVIEDPSGTLVGMASDGGTGNEGALFK
                     YTISGSVGTVLLPFSYANGANPHGRLFKASNGLFYGLASAGGEFSSGTLFSIDPTNSN
                     FITRVHFDNGKGTIPLGSLVEDNGKLYGVCSAGGTANGGTLFEYNISSNILSVKVNFA
                     STLVGNAPVNGLFKATNGLMYGATGAGAGNAQGSLYEYVPGGTTLTKRKDFLLATTGK
                     QPLGDVMQASDGLLYGTTSLGGAENYGTIFSFDPVSNTFTTIYNFNGLEGGTPGGELL
                     QATNGKLYGVFREEGQGFSGGIYSWDIGTATYTEEFDLNIAPVTDQGSLPQSSLLQGS
                     DGMLYGTTTQGGSGGNASGAIFRFDPTSLAYSVLLTFTGASNGALPYDGLVPESAVIP
                     QSVGLAAKVFLEGPFVSGTGLMNANLRSLPSFPLTEPFSALGFTQVGGGGETIAPSVL
                     ATSGN"
BASE COUNT          435 a          707 c          667 g          427 t
ORIGIN      
        1 caatgggctg tattacgggg ttaccgaggt cggtgggaca ggtggggtgg gcgtgctctt
       61 cagtttcaac ccgtccgacg gcacgtacac cgtgctccac aatttcaatt catctaccac
      121 gggggcacag ccgctgcgcg gcccggtgat cgcgaacaac ggcaggttgt acggcacgtg
      181 cagcagtgga ggggccaaca atgtgggcac gttgtgggag tacaacatcg gcacctccac
      241 cctcaccaag aagttcgatt tcgatggact gacaacgggc aaggggagca ccccgcgcgg
      301 ccggttgatg gtacacagca acggcacgat ctacgggacc acccagcttg gtggcctgaa
      361 cggggcgggt tgcatcttct cgtacacggc ggggggcgtg agcaacacca aggtgtacaa
      421 cttccccgca ctgccggcga tcacaacggg caatcggccc cttaccggcc ttgcccagtc
      481 gggaagcctc ttgtacggtt tcaccgccaa cggcggggcc aatggccatg gctccatctt
      541 ttcattcaac accagtggta acgttttcac gaacctgtac aacttcctgg ccacgggtgc
      601 cggacgcgcc cccctctccg agttcacggc tgtttccggg gtcctgtacg ccaccgcatc
      661 ggccggaggc tcaagtagtg gaggcaccat cttcagttgg gatatcagcg gtgccgcgca
      721 tgccgatctg gtgcacctca acaacagcat cggttatgca ccgttctcgc ggctgttcgc
      781 ggccagtgac ggacaacttt atgggaccac cagcgcgggc ggcacctcga atgccggggt
      841 cctgttctcc ttcaatacgg gcaccaacac ttacacgccc gtgcttcaga tggcctcact
      901 cggactgtcc gcaccatgga gcagcgtgat cgaggacccc tccggcacgc tggtgggaat
      961 ggccagtgac ggcggtaccg gcaacgaggg agcactgttc aagtacacca tctccggttc
     1021 ggtcggtacc gtgctgcttc cgttcagcta cgccaacggg gccaaccccc acgggcggtt
     1081 gttcaaggcc agcaacgggc tcttctatgg gctcgcgagc gcggggggcg agttctccag
     1141 cggcaccctg ttcagcattg acccgaccaa ctccaatttc atcacccggg tgcattttga
     1201 caatggcaaa ggcaccatcc cgttgggcag cctggtggag gacaacggca aattgtacgg
     1261 cgtttgcagt gctggcggta ccgccaatgg cggcacattg ttcgagtaca atatcagctc
     1321 caatatcctg tcggtcaaag tgaacttcgc atccaccttg gtgggcaacg cccccgtcaa
     1381 cggtctgttc aaagcgacca acgggttgat gtacggtgcg accggtgcgg gtgcgggcaa
     1441 cgcgcaggga tccctgtatg aatatgtacc gggcggtacc accctcacca agcgcaagga
     1501 cttcctgttg gccaccaccg gcaagcagcc cttgggcgat gtgatgcagg catccgacgg
     1561 cctgttgtac gggaccacca gtttgggcgg cgccgagaac tacgggacca tcttctcctt
     1621 cgatccggtc tccaacacct tcaccacgat ctataacttc aatggacttg agggcggcac
     1681 accgggaggt gaactgctgc aagccaccaa cggcaagctg tacggtgtgt tccgcgaaga
     1741 aggacagggc ttctccggcg ggatctacag ctgggacatc gggaccgcca cgtacaccga
     1801 ggaattcgac ctcaacatcg ccccggtaac ggaccagggc agtctgcccc agagttcgct
     1861 gctccagggg tcggatggca tgctgtacgg caccaccacg caaggaggct ccggaggcaa
     1921 tgcttcgggt gcgatcttcc gtttcgaccc caccagcctc gcttattcgg tgctgctcac
     1981 cttcaccgga gcgtccaacg gtgcactgcc atacgatggt ctggtgcccg aatccgcggt
     2041 gatcccgcaa tcggtggggc ttgctgccaa ggtgttcctt gaaggcccct tcgtcagcgg
     2101 aaccggcctg atgaacgcca acctgcgttc actgccctct ttcccactca ccgaaccctt
     2161 cagcgcgctg ggattcaccc aggtcggagg aggaggcgag acgatcgccc cgtccgtgct
     2221 tgccacttcg ggaaat
//
LOCUS       DAOQOC010000468         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30598_length_2232_cov_3.379881, whole genome shotgun sequence.
ACCESSION   DAOQOC010000468 DAOQOC010000000
VERSION     DAOQOC010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30598_length_2232_cov_3.379881"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PL106_08145"
     CDS             <1..322
                     /locus_tag="PL106_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HNA33078.1"
                     /translation="GMDECLARLGVKDRFFNVADKSAKLDELIASGLDPSRTAYMGDD
                     IPDLRAMTRVALPCCPADAAEEVKAISRYISPKPGGMGCVRDLLEQALKARGRWLTEN
                     AHTW"
     gene            325..1377
                     /locus_tag="PL106_08150"
     CDS             325..1377
                     /locus_tag="PL106_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNA33079.1"
                     /translation="MIDLIRLTRPLTLLIMALTMAAMRYGVVGAWLDHTSAAIQSTSI
                     DPTGTTIAEIPGNRLMHAFTGVHFWLLVLSTLLIAAGGNVINDYFDTRIDRVNKPAQV
                     IVGRTVKRRVAMAGHLVLSGLGLAIGAFVAWRSGQFRLVVIPAFAVIALWSYSTRLKR
                     AFLIGNGTVAVLAALVPITVGLYEVTALARTYPRDNWLSTVNGELLKVVIDFNEPWYW
                     ILGYGLFAFLATLVREIQKDLADVEGDRAEGCRTLPIVLGMRWGKAVALTYMGLLLVC
                     VLYARMALLHDRLSYWYIGIAVITPLLLSAGFTYNASTRREHGIASHLLKAAMVIAIL
                     YAFLLRHTIWDPARLL"
     gene            1353..1958
                     /locus_tag="PL106_08155"
     CDS             1353..1958
                     /locus_tag="PL106_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNA33080.1"
                     /translation="MGPCTPPLSLRLILASASPRRRQLMAGLDLPLEVVGINVDETPP
                     PGMAAEAVAEHLARKKADAWAGTLAPDQVLITADTTVLVNAELLNKPEDEADARRMLR
                     MLSGREHRVITGVCLRSAVRTLSFADTAVVRFRALSDVEIDYYVRTHRPMDKAGAYGV
                     QDWIGYTAVERIEGSFYTVMGLPMHRVYQGLKELMPVQDAV"
     gene            complement(1969..>2232)
                     /locus_tag="PL106_08160"
     CDS             complement(1969..>2232)
                     /locus_tag="PL106_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33081.1"
                     /translation="ALYAPDAFTPNGDGHNETFAVVTSVREPSFFRLRVFDRWGTALF
                     TSTDPFVGWSGDGTAIGIYAWAVELLDSEGKPRKATGHVTLLR"
BASE COUNT          388 a          703 c          741 g          400 t
ORIGIN      
        1 aggcatggat gagtgcttgg cgcgcctcgg cgtgaaggat cgcttcttca atgtggctga
       61 caagagtgcg aaactggacg agctgatcgc gagtggcctc gatccgtcgc gcacggcgta
      121 catgggcgac gacattcccg acctgcgcgc gatgacgcgg gtggccctgc cgtgctgtcc
      181 cgccgatgcg gccgaagagg tgaaggccat ctcccgctac atcagtccga aacccggggg
      241 catgggctgc gtgcgcgacc tgctggagca ggccctgaaa gcacgcgggc gctggctcac
      301 cgagaacgcg catacctggt aggcatgatc gacctcatcc ggctcacccg accgctcacc
      361 ctgctgatca tggcgctcac catggccgcc atgcgctatg gagtcgtggg tgcatggctg
      421 gaccacacca gtgcggcgat ccaaagcacg agcatcgatc cgacggggac caccatcgcc
      481 gagatccccg gcaatcgcct gatgcacgcc ttcaccgggg tgcatttctg gttgctcgtg
      541 ctgagcacac tgctcatcgc ggctggaggg aacgtcatca acgactactt cgatacgcgg
      601 atcgatcggg tgaacaagcc cgcacaggtg atcgttggcc gcaccgtgaa gcgccgtgtc
      661 gccatggccg ggcacctcgt cctcagcggg ctgggcctcg cgatcggcgc gttcgtcgca
      721 tggcgcagcg ggcagttccg cctcgtggtg atacccgcgt tcgccgtgat cgcgctgtgg
      781 agctacagca cccggctgaa acgcgcattc ctgatcggga atggcacggt ggccgtgctg
      841 gcggccctgg tgcccatcac ggtggggctg tatgaggtaa cggcccttgc gcgcacgtac
      901 ccccgagaca actggctcag caccgtgaac ggggaactgc tgaaggtggt gatcgacttc
      961 aacgagccgt ggtactggat cctcggctac ggcctgttcg ccttcctggc caccctggtg
     1021 cgcgagatcc agaaagacct ggcggatgtg gagggcgacc gcgccgaggg ctgtcgcacc
     1081 ctgccgatcg ttctgggcat gcgctggggc aaggccgtag cgctcacgta catggggttg
     1141 ctgctcgtgt gcgtgctgta cgcccgtatg gccctgcttc acgaccggtt gagttactgg
     1201 tatatcggga tagctgtgat cactccgctg ttgctgtcgg cgggattcac ctacaacgcg
     1261 tcaacgcgtc gcgagcacgg catcgccagc cacttgctca aggcggccat ggtcatcgcg
     1321 atcctgtacg cctttctctt gcgccatacg atatgggacc ctgcacgcct cctctgagcc
     1381 tccgtctgat cctggcatcc gcctcaccac ggcgccgtca gttgatggcc gggctggatc
     1441 ttcccctgga ggtcgtgggc atcaacgtgg acgaaacgcc accaccgggc atggccgccg
     1501 aagcggtggc cgagcacctc gcgcgaaaaa aggccgatgc gtgggcaggt acgctggccc
     1561 cggatcaggt gctgatcacc gcggatacca cagtgctcgt gaacgcggaa ctgctgaaca
     1621 agcccgagga tgaggcggat gcccgccgca tgctgcggat gctcagcggt cgtgagcatc
     1681 gtgtgatcac cggcgtctgc ctgcgttcgg cggtgcgcac cctgagcttc gccgacaccg
     1741 ccgtggtgcg tttccgtgcg ctgagcgatg tcgagatcga ctactacgta cggacgcacc
     1801 ggccgatgga caaagcgggg gcctacggtg tgcaggattg gatcggatac acggccgtgg
     1861 agcggatcga gggcagcttc tacaccgtga tgggcctgcc catgcatcgc gtgtaccaag
     1921 gcctgaagga attgatgccc gtgcaggacg cggtctgagg ggccatgttc agcgaagcag
     1981 cgtcacatgt ccggtcgcct tgcggggttt gccctccgaa tcgagcagtt ccacggccca
     2041 tgcgtagatc ccgatggcgg tgccatcgcc ggaccagccc acgaacggat ccgtggacgt
     2101 gaacaacgcg gtgccccacc ggtcgaacac gcgcaatcgg aagaacgagg gttcgcgaac
     2161 gctggtgacc acagcgaagg tctcgttgtg accatcgccg ttgggggtga acgcatccgg
     2221 cgcgtacagt gc
//
LOCUS       DAOQOC010000469         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30622_length_2231_cov_3.716452, whole genome shotgun sequence.
ACCESSION   DAOQOC010000469 DAOQOC010000000
VERSION     DAOQOC010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30622_length_2231_cov_3.716452"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PL106_08165"
     CDS             complement(<1..471)
                     /locus_tag="PL106_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33082.1"
                     /translation="MTLLRYLTLLCTAIAVLATQAQSTFGYTYASAGALASQYLLHAS
                     SYGASGQLIPFDIAHGIATVKVDAAGDVAWSNALAALDTTFRMSVTSSTVSQTGDGLL
                     VGPLYASGSPGASEIGLIDLGPDGVPQWSVRIAIDSHPFAIEDFMPPLVATTANG"
     gene            complement(572..823)
                     /locus_tag="PL106_08170"
     CDS             complement(572..823)
                     /locus_tag="PL106_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33083.1"
                     /translation="MVLWTGNVNGDDRVKYTGSGNDRDPILISIGSIAPNNTISGYVF
                     EDVNLDGLVKYTGSGNDRDRVLQTIGSIVPSNVRVEQLP"
     gene            776..1006
                     /locus_tag="PL106_08175"
     CDS             776..1006
                     /locus_tag="PL106_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33084.1"
                     /translation="MFHTIVAVHVAGPQHHGPVRALACIGPKIMARETTKALRKDVTA
                     KCYGGDVSRKRKLLEKQKEGKKMKQIGTVEIL"
     gene            complement(1048..>2231)
                     /locus_tag="PL106_08180"
     CDS             complement(1048..>2231)
                     /locus_tag="PL106_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33085.1"
                     /translation="YVLLRQTLADTDLTAVVTANGAVQVVVRYAFEASDRITVLALTS
                     EGELSWSKSYSFSTSPDDRCWAGAIAAPADGGSLIAGSASSDIYLIRLDESGAEVWDR
                     RIYCGQFLEVYSAVPTSDDGFVIAGNLNDLAPEVFAMKVSSSGSIEWMKHYGDAGNTR
                     GFWNMVTDEQGSIWAGEYYSIGTRLMQLSPDGEVEDAFTLTGMSDVSCAYPIVLRVAE
                     DTVTAVFSRQTDCDDPHPTSATLLRFAASSLEPCHLAPNVLSAALVPGSTVLGVTTYV
                     IDETVSVLPLPISVDPVVTSRTSVCQTTAITTPEVTDPGKLAVHPSLVATGASFTLTL
                     PSTEMRETLLMDDLGRAVVRTVTANAHSLEVSTAGLAPGYYHIQVRSARDVLRAAVVV
                     E"
BASE COUNT          457 a          678 c          672 g          424 t
ORIGIN      
        1 gccgttggcg gtggtggcca ccaggggtgg catgaagtcc tcgatcgcga acggatgcga
       61 gtcgatcgcg atcctcacgc tccattgtgg aacaccgtcc ggtccgagat cgatcagccc
      121 gatctcgctc gcgcctggcg aacccgacgc gtacaacggg ccgaccagca ggccgtctcc
      181 ggtctggctg accgtggatg aggtgacgct catccggaag gtggtatcca atgctgccaa
      241 cgcattgctc caggccacgt cgccagcagc atccaccttc acagtggcaa tgccatgcgc
      301 gatatcaaag ggtatcagct gcccgctcgc accataggag gatgcatgca acaggtattg
      361 agaagccagc gctcctgcgg aagcgtaggt gtagccgaat gttgattggg cctgggtagc
      421 tagcaccgcg atcgcggtgc agagcagggt cagatatcgg agcagggtca tggccgggtc
      481 tttgccaata gacgtgcgct acggcgaaac gctgcctggt cacggtcccg cgtacccgga
      541 aatgcttccc tgataagatc cggcgttagc cctacggcaa ctgctccacc cgcacgttcg
      601 agggcacgat gctgccaatg gtctgcagca cgcggtcgcg gtcgttgccc gatcccgtgt
      661 acttgaccag tccatcgagg ttcacgtcct cgaacacgta gccgctgatc gtattgttgg
      721 gcgcgatgct cccgatggag atgaggatgg gatcacggtc gttgccgctt ccggtatatt
      781 tcacacgatc gtcgccgttc acgttgccgg tccacagcac catggtcccg ttcgcgctct
      841 ggcgtgcatc ggtcccaaga tcatggcgcg cgaaaccacc aaggccctcc gcaaggacgt
      901 taccgcgaag tgctatggcg gtgatgtctc acgaaagcgg aagctgctgg agaaacagaa
      961 ggaagggaag aagatgaagc agatcggcac cgtggagatc ctgtagcagg cgttcctcgc
     1021 tgtgttgaag ctggactggt caccggctca ctccacgacc accgcagcgc gcagcacgtc
     1081 tcgggcgctc ctgacctgga tgtgatagta tcccggcgcc agtcctgctg tcgaaacttc
     1141 caggctgtgt gcgttggcag ttactgttcg cacgacagcg cgtcccagat cgtccatcaa
     1201 cagggtctcc cgcatctccg tcgacggaag ggtcaacgtg aagcttgctc ccgtcgcaac
     1261 gagcgatgga tgaacggcca atttgcccgg gtcagtgacc tccggagttg tgatcgccgt
     1321 agtctgacaa accgatgtgc gcgacgtgac aaccgggtca acgctgatcg gcagcggcag
     1381 cacgctcacg gtctcatcaa ttacatacgt ggtgacaccc aacacggtgg atcccggaac
     1441 aagagctgct gagagcacat tcggtgccag atggcagggt tccagtgaac tggctgcgaa
     1501 ccgcagtagc gtggcgctcg tggggtgtgg atcgtcgcaa tccgtttgtc gcgagaagac
     1561 cgccgtaacc gtgtcctccg ccacacgaag cacgatggga taagcgcacg aaacatccga
     1621 catgcctgtg agggtgaagg cgtcctccac ttcaccgtcc gggctcagtt gcatcaagcg
     1681 tgtgccgatc gagtagtact cacccgccca gatggaacct tgttcatccg tcaccatgtt
     1741 ccaaaagccg cgcgtattgc cggcatctcc ataatgcttc atccattcaa tggagccgct
     1801 ggagctcacc ttcatggcga agacctctgg cgcgaggtcg ttcaaattac cggcgatgac
     1861 gaagccgtcg tcgctggtag gcacagccga atagacctca aggaactgac cgcagtagat
     1921 acgcctatcc cacacctcag cgccgctttc gtccagtcgg atgaggtaga tatcactact
     1981 tgcgctgccc gcgatcaagc tgccgccatc cgcaggtgca gcgatcgctc cggcccagca
     2041 tcgatcgtcc ggtgaggtgg agaagctgta gcttttgctc caggacaact cgccttcgga
     2101 tgtgagcgca agcaccgtga tgcgatccga ggcctcgaac gcatagcgca caacgacctg
     2161 gacggcgccg ttggcggtga cgaccgcggt gagatcggta tcggccagcg tctgccgcaa
     2221 caacacgtac g
//
LOCUS       DAOQOC010000470         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30701_length_2228_cov_3.915785, whole genome shotgun sequence.
ACCESSION   DAOQOC010000470 DAOQOC010000000
VERSION     DAOQOC010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30701_length_2228_cov_3.915785"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PL106_08185"
     CDS             <1..294
                     /locus_tag="PL106_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33086.1"
                     /translation="SYDVALAGYKTGKYEEAIVKFSEVLATDTNNLADAYYYRGDCYY
                     HLKNKEKACADFSACAALKDKDAVFIRKNYCETDLGHIPKKPVRKRRRTMVEF"
     gene            442..1167
                     /gene="gldF"
                     /locus_tag="PL106_08190"
     CDS             442..1167
                     /gene="gldF"
                     /locus_tag="PL106_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNA33087.1"
                     /translation="MRALLRKEIRGFLGSLIGHVVIVVFLLITGLFLWVFPDNILDLG
                     YADLGPLFFLAPWVFLFLVPAITMRSFSEERRTGTLELLLTKPISEFTLVLGKYTAAV
                     LLVLLALIPTLVYYWSVGQLAVPTWNIDAGGTWGSYLGLFFLAACFTAIGVFASTLTD
                     NQIVAFLIAAVLCFGLFMGFELLASFDAVGGLEGPLKNIGIQQHYASMSRGVVDLRDV
                     LYFGGVIVVFLLLTRTVVQSRKW"
     gene            1183..>2228
                     /locus_tag="PL106_08195"
     CDS             1183..>2228
                     /locus_tag="PL106_08195"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNA33088.1"
                     /translation="MRSSPRSRDLLSLLLGIGCVLLVLFISSFARVRADLTSEKRFTL
                     TPATCDLMDSLKDVVYLQVYLAGELPADLRKLGEATRELLDEMRVHNPEMLQFTFTDP
                     SAAPDEKSRLANYAKLQEEKLEYTSIRTEDKGAASERIVWPCAMLNYKGRQQPLQLLR
                     TQFRTPDAGMVNRSINNLEYEVASAIRKIVSDHRPRVAILASNGCLDELSVKDIGTAL
                     SEQYEVSRVRIDGRISALSDKVEGGRYRANKFEALIVPKPDSAFSDKDAYIIDQFIMN
                     GGRVLWAVDAMNAHLDSLRTRQYSIATPLDLGLDKLFFAYGTRLNKDLLLDAQCAPIQ
                     FYGDKFGNQRKLETR"
BASE COUNT          418 a          740 c          664 g          406 t
ORIGIN      
        1 agctacgacg tggcactcgc cggctacaag accggcaagt acgaagaggc catcgtgaag
       61 ttctccgagg tgctcgccac ggacaccaac aacctggccg acgcttatta ctaccggggc
      121 gattgttact accacctcaa gaacaaggag aaggcctgcg ccgatttcag cgcctgcgcc
      181 gcattgaagg acaaggacgc cgtcttcatc cgcaagaact attgcgagac cgacctgggc
      241 cacatcccca agaagccggt gcgcaagcgc aggaggacga tggtggagtt ctgagcccag
      301 gggtccgtgt acgcatgtgc cgttcggcac atggaccatc ggcgcacgag agatccctgt
      361 gccaacgacc accggtggac agccgttcct cgttgtgtac ggcccggtac ctgcacgccc
      421 ctaccttggt cgccgcaacc gatgcgtgcg ctcctccgca aagagatccg tggcttcctc
      481 gggtcgctca tcggccacgt ggtcatcgtg gtcttcctgc tcatcaccgg gctgttcctg
      541 tgggtgttcc ccgacaacat cctggacctg ggctacgccg acctcggccc cctgttcttc
      601 ctggccccgt gggtcttcct cttcctggtg cccgccatca ccatgcgcag cttcagcgag
      661 gaacggcgca ccggtacgct ggaactgctg ctcaccaagc ccatcagcga gttcaccctc
      721 gttctgggca agtacaccgc cgccgtcctg ctggtgctgc tggcgctgat accaaccttg
      781 gtgtattatt ggagtgttgg ccagctggcc gtgcccacct ggaacatcga tgcgggcggc
      841 acttggggca gctacctcgg gctcttcttc ctggccgcct gcttcacggc catcggcgtg
      901 ttcgcctcca ccctcaccga caaccagatc gtggccttcc tgatcgccgc cgtcctctgc
      961 ttcggtctct tcatgggctt cgagctgctg gcctcgttcg acgccgttgg cgggctggaa
     1021 ggaccgctca agaacatcgg catccagcag cattacgcca gcatgagccg cggtgtggtc
     1081 gacctgcgcg acgtgctcta cttcggcggg gtgatcgttg ttttcctgtt gctgacgcgc
     1141 acggtggtac agagcagaaa atggtgatcc actgaccgga ccatgagaag cagcccacgc
     1201 tcccgcgacc tgttgtccct gttgttgggt atcggctgcg tactgctcgt gctgttcatc
     1261 agctcgttcg cccgggtgcg cgccgacctg accagcgaga agcgcttcac gctcacaccg
     1321 gccacttgcg acctgatgga cagcctgaag gacgtggttt acctgcaggt gtacctggcc
     1381 ggtgaactgc ccgccgacct gcgcaagctg ggcgaggcca cccgcgagct gctggacgag
     1441 atgcgggtac acaacccgga gatgctgcag ttcaccttca ccgatcccag cgcggcgccc
     1501 gacgagaagt cgcgcttggc gaactacgcc aagttgcagg aggagaagct ggagtacacc
     1561 agcatccgca cggaggacaa gggcgcggcg agcgaacgca tcgtgtggcc ctgcgccatg
     1621 ctcaactaca agggccgaca gcaaccgttg caactgctgc gcacgcaatt ccgcaccccc
     1681 gatgcgggca tggtgaaccg cagcatcaac aacctggagt acgaggtggc cagcgccatc
     1741 cgcaagatcg tttccgatca ccggccgcgc gtggccatcc tcgccagtaa cggctgtctg
     1801 gacgagttga gcgtgaagga catcggaacc gcgctgagcg agcagtacga ggtgagccgc
     1861 gtacgcatcg atggccgcat cagcgccttg agcgacaagg tggagggcgg acgctaccgc
     1921 gccaacaagt tcgaagccct gatcgtgccc aagccggaca gcgcgttcag cgacaaggac
     1981 gcgtacatca tcgaccagtt catcatgaac ggcggtcgtg tgctctgggc cgtggacgcc
     2041 atgaacgcgc acctcgacag cttgcgcacc cggcaatact ccatcgccac accgctggac
     2101 ctggggctgg acaagctctt cttcgcctac ggcacccggc tgaacaagga ccttctgctc
     2161 gatgcgcaat gcgcacccat ccagttctac ggcgacaagt tcggcaacca gcgcaaactg
     2221 gaaacgcg
//
LOCUS       DAOQOC010000471         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30718_length_2228_cov_2.780488, whole genome shotgun sequence.
ACCESSION   DAOQOC010000471 DAOQOC010000000
VERSION     DAOQOC010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30718_length_2228_cov_2.780488"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PL106_08200"
     CDS             complement(<1..202)
                     /locus_tag="PL106_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33089.1"
                     /translation="MKRILVPILSAAVLLSSCVSKKQYSALQASNTTLNQKLADCNIA
                     LDGCRGEKAVLDARIAELENKNQ"
     gene            472..>2228
                     /locus_tag="PL106_08205"
     CDS             472..>2228
                     /locus_tag="PL106_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA33090.1"
                     /translation="MSHVRLLLLSSTVLFAIGARGQAHSITEPQITTCTGALLDSGGQ
                     GASGYSDNEDFTSTICSDQPGQAITLNWITFNLNTDGGDPIDQLTIYDGPDNTAPVIG
                     TYTGTNSPGIVSASFANTSGCLTLHFTSNSTGTGIFAATILCYTPCEPPTAVVTMSEP
                     APALICQGEALQFNASGSYAAQGYNVVEYAWNFDDGSVVDSTTGPVVSHAFQEPGEYI
                     VQIVLTDDNNCANTNTIDLAVQVSTTPVFNLSDTSVCLGNDVPLNGEAVPTTWTALPG
                     DDLGGGVFLPDNVGETFITQLNYNNVFAPGATLQNIDQLLTFCINMEHSFMGDIVIQL
                     VCPNGQSVVVHQQGGGGTFLGEPVDDDLQPDAQGVCYNYCWSPTATNGTWVDNSGGTL
                     PPGTYESLNPMSALQGCPLNGTWTLNITDLWGSDNGFVCDWGLDFDPALYPDLTTYTP
                     VLGLSTTDSTFWSGDGVVLDSNDPTTASVTPTSPGSNIYVFTVTDNFGCTYSDSITVT
                     ASGPLAQFDVQPVSPQPVGITAQFTDTSVPNNTTITGWNWDFGNGTSSTVQNPVTTFS
                     EPGTYTVTLTITSSDGCTST"
BASE COUNT          458 a          732 c          620 g          418 t
ORIGIN      
        1 gctggttctt gttctcgagt tcggcgatcc gggcgtcgag cacggccttt tcaccacggc
       61 aaccgtcgag cgcgatgttg cagtcggcga gcttctgatt cagcgtggtg ttgctggcct
      121 gaagagccga gtactgcttt ttggacacac aggaggagag caggacagct gcgctgagga
      181 tcggcaccag gatacgcttc atgcgggagg tcgggttgtg gtttttcggg cgccgaagat
      241 agcccccacc ccctttgacc gttcgttcgt gagacgtatt gtacgctgcg cgatcccacc
      301 cggaaccacg taccgaccgg cttcatcgaa aggcccggct gtccgatcca gccccgcgtg
      361 ccggggtgct cacctacatt cgcgaccccc ggatcggggt ccttggcaac agcacgagcc
      421 tcttatgcgt catagccccg acccttcacc acgaaccacc cccatcacgc tttgagccac
      481 gtacgtcttc tcctgctgtc aagcaccgtc cttttcgcca ttggcgcccg aggccaggcg
      541 cacagcatca ccgaacccca gatcaccacc tgcaccgggg cactgctcga tagcggaggt
      601 caaggcgcaa gcggttatag cgacaacgag gatttcacct ccaccatttg ctcggaccaa
      661 ccgggtcagg cgatcacgct gaattggatc accttcaacc tgaacacgga tggtggcgac
      721 ccgatcgatc agctgaccat ctacgacggt ccggacaaca cggcaccggt catcggcacc
      781 tacaccggca ccaactcgcc aggtatcgtc tcggccagtt tcgcgaacac gagcggctgc
      841 ctgaccctgc acttcaccag caattccacg ggcacgggga tcttcgcggc caccatcctg
      901 tgctacacgc cctgtgagcc gcccacggcg gtggtcacca tgagcgaacc tgcacccgca
      961 ctgatctgcc aaggtgaagc actgcaattc aacgcctcgg gatcatacgc ggcccaaggc
     1021 tacaacgtgg tggagtacgc ctggaacttc gacgacggca gcgttgtgga cagcaccacc
     1081 ggccccgttg tgagccatgc gttccaggag cccggcgagt acatcgtaca gatcgtgctc
     1141 accgacgaca acaactgcgc gaacaccaac acgatcgacc tcgcggtaca ggtgagcacc
     1201 acaccggttt tcaacttgtc ggacacctcg gtctgtttgg ggaacgatgt gcccttgaat
     1261 ggggaagctg ttcctaccac ttggacggcg cttccgggcg atgatctggg gggcggagta
     1321 ttcctgccgg acaatgtggg tgaaacattc atcacccaac tgaactacaa caacgtgttc
     1381 gcacccggtg ccacgctgca gaacatcgat cagttgttga ccttttgcat caacatggaa
     1441 cactcgttca tgggcgacat cgtgatccaa ctggtgtgcc cgaacggaca gtcggtggtg
     1501 gtacaccaac aaggcggcgg gggcaccttc ctgggcgaac ccgtggatga tgatctgcag
     1561 ccggatgcgc aaggcgtgtg ttacaactac tgctggtcgc ctaccgcgac gaacggcacg
     1621 tgggtggaca actccggagg tacgttgccc cctggcacat acgagagcct gaaccccatg
     1681 tccgccctgc aaggctgccc gctcaacggc acttggacgt tgaacatcac cgatctgtgg
     1741 ggaagtgaca acgggttcgt gtgtgattgg ggcctcgatt tcgaccccgc gctctacccc
     1801 gacctgacga cctacacccc ggtcctaggg ctgagcacca cggactcaac cttctggtca
     1861 ggcgacggtg tggtgttgga cagcaacgac cccacaaccg cgagcgttac gcccacctcg
     1921 ccgggcagca acatctacgt gttcacggta acggacaact tcggatgcac ctactcggat
     1981 tccatcacgg taacggccag cggcccgctc gctcaatttg atgttcagcc cgtatcgccg
     2041 cagcccgttg gcatcaccgc tcaattcacg gacacatccg tacccaacaa taccacgatc
     2101 accggttgga actgggattt cgggaacggc acctccagca ccgtgcagaa ccccgtcacc
     2161 acgttcagcg agcccggcac ctacacggtg accttgacga tcacttcgtc ggacggttgt
     2221 accagcac
//
LOCUS       DAOQOC010000472         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_30771_length_2226_cov_3.184247, whole genome shotgun sequence.
ACCESSION   DAOQOC010000472 DAOQOC010000000
VERSION     DAOQOC010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30771_length_2226_cov_3.184247"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..308)
                     /locus_tag="PL106_08210"
     CDS             complement(69..308)
                     /locus_tag="PL106_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33091.1"
                     /translation="MSAAYNTWHLTAELTVQELYIVLGYAWYMSATSTGDVMAAHNRV
                     AQAAKSALVAAGETHERIYSKLEAIMLEMEIRPSE"
     gene            complement(310..582)
                     /locus_tag="PL106_08215"
     CDS             complement(310..582)
                     /locus_tag="PL106_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016049872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HNA33092.1"
                     /translation="MNLNFGQALEALKSGHRVARSGWNGKNMFLFLTKGRVVTNDKRG
                     SFAHFPGNTVELRSHIDMFDAQGCYVSGWLASQTDMLAEDWTILPV"
     gene            complement(585..1211)
                     /locus_tag="PL106_08220"
     CDS             complement(585..1211)
                     /locus_tag="PL106_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33093.1"
                     /translation="MNRKAFLYLFVGTNGTGKTTRAKDLLDINPRNLIVPSNRLDSAW
                     SRYPELAITVEHEDDPMNPGKQRRVVRCPEMASFTGTRVLHIDNPAKLQAITDERFGF
                     RNGLLFIDDFRNRMPHKGDLPSDVLNMLTGRRHRMLDIALAMHSFQDMNLQLMQFQPE
                     LFIFRTTLPPNDAVEGKVADWQGLVRTANRVNDRAKDNRYYFEHFIPA"
     gene            complement(1208..2035)
                     /locus_tag="PL106_08225"
     CDS             complement(1208..2035)
                     /locus_tag="PL106_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33094.1"
                     /translation="MSEQTLLPPHTESAEGDSPGNGFNLSAHADRLKAQPKEHYEQPA
                     EPPPPPPPEPEPAKEPEGGGEGPKSTADDGASARFIIDMYDAGVSKLSEAMVDEPGRY
                     PAKYFAMEPMLKEHAVAQLAKGMQEGGGKWKMPWWVALLIVLLFHGGLTWAAVRSAKR
                     EKADRKAQDKAKANERDRAGRAAPSSGRGEPSPAGHTPFTIIDRNGNTLHPRPPASAA
                     AHTTCQREGCNTPVKRGRKYCSQQCAGLATRGRRRDMATPEPPRKDTPEPAAAATTT"
     gene            complement(2032..>2226)
                     /locus_tag="PL106_08230"
     CDS             complement(2032..>2226)
                     /locus_tag="PL106_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33095.1"
                     /translation="IIQAAGGDPVTDGAYLWRQAKANGKETIMATVYRRNELDEPGDI
                     IGTIDVLASVERMDLSQFIQ"
BASE COUNT          328 a          666 c          731 g          501 t
ORIGIN      
        1 ctccgccttc acgctcaaag tgactttgcg tttcatggcc cgaaaatacg tatgagtgga
       61 tacggatgtc attcgctggg ccggatctcc atttccagca tgatcgcctc cagcttgctg
      121 tagatgcgct catgtgtctc accggcagcc accagagcgc tcttggcggc ctgggccacg
      181 cggttgtggg cggccatcac atcgccggtg ctggttgcgc tcatatacca ggcatagccc
      241 agcacgatgt acagttcctg tacggtgagt tcggcggtga ggtgccaggt gttgtaagcg
      301 gcgctcatgt cacacaggga gaatggtcca gtcttcggca agcatgtcgg tctggctggc
      361 cagccatccg ctcacatagc atccttgcgc atcgaacatg tcgatgtgag agcgaagctc
      421 tactgtattt ccgggaaagt gagcaaagct cccccgctta tcgttcgtta ctacgcgacc
      481 cttcgtgagg aagaggaaca tgttcttccc gttccagccg gagcgggcaa ctcgatggcc
      541 gctcttcagg gcttcgagtg cttggccgaa gttcaggttc atggtcaggc aggtatgaag
      601 tgctcgaaat agtaccggtt gtccttggcg cgatcgttca cgcggttggc ggtgcgcacc
      661 aggccctgcc agtcggccac cttgccctcc acggcatcgt tgggcggcag cgtagtgcgg
      721 aagatgaaca gctcgggctg gaactgcatg agctggaggt tcatatcctg gaaactgtgc
      781 atcgccaggg cgatatccag catgcggtgc ctgcggccgg tgagcatgtt gagcacgtcg
      841 ctgggcaggt cgcccttgtg gggcatgcgg ttgcggaagt cgtcgatgaa cagcaggccg
      901 ttgcggaagc cgaagcgctc atcggtgatc gcttggagct tggccgggtt gtcgatatgg
      961 agcaccctgg ttcccgtgaa ggaggccatt tctgggcagc gcaccacgcg gcgctgcttg
     1021 ccggggttca tggggtcgtc ctcatgttcc acggtgatcg ccagctcggg gtatcggctc
     1081 cacgcactat cgagccggtt gctcggcacg atcaggttcc tggggttgat gtccagcagg
     1141 tccttagcgc gggtggtctt gcccgtgccg ttggtgccca cgaagaggta caggaaggct
     1201 ttgcggttca tgtggtagtg gcggcggcgg ctggttccgg cgtgtccttc ctgggtggct
     1261 caggtgtggc catgtcgcgg cgtcggcctc gggtggccag ccccgcgcat tgctgcgagc
     1321 aatacttgcg gccgcgcttc accggggtat tgcagccctc gcgttggcag gtggtgtgag
     1381 ctgcggcgct ggccggtggg cgcgggtgca acgtgttccc gttgcgatcg atgatagtga
     1441 agggggtgtg accggccggc gacggctcac ctctgccact gctcggggcc gcacgtccag
     1501 ctcggtcacg ttcgttcgcc ttggccttgt cctgggcctt gcggtccgcc ttctcgcgtt
     1561 tcgcactgcg cacagccgcc caggtgaggc ccccatggaa cagcagcacg atgagcagcg
     1621 ccacccacca gggcatcttc catttgcccc caccctcctg catgcccttg gcgagctggg
     1681 ccaccgcatg ctctttgagc atcggttcca tggcgaagta cttggccggg taccgcccgg
     1741 gttcgtccac catagcctcg gagagcttgc taacccccgc atcgtacatg tcgatgatga
     1801 agcgggcact ggccccatcg tcggccgtgc tcttcggtcc ttctccccca ccttccggtt
     1861 ccttggctgg ttcaggttcg ggtggcggcg gcggcggtgg ttcagcaggc tgctcgtagt
     1921 gctccttggg ctgtgccttc agccggtcgg catgtgcgga caggttgaag ccgttccctg
     1981 gactgtcacc ctcagcgctt tctgtgtgcg gcggtagcag cgtctgttcg ctcattggat
     2041 gaactgggat aggtccatgc gctccacact ggcgagcacg tcaatggtgc cgatgatgtc
     2101 gccgggttca tccagctcgt tgcggcggta cacggtggcc atgatcgtct ccttgccgtt
     2161 cgctttcgct tgcctccaga ggtaggcccc atcggttacg gggtcgcccc ctgcggcctg
     2221 gatgat
//
LOCUS       DAOQOC010000473         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31025_length_2217_cov_3.364477, whole genome shotgun sequence.
ACCESSION   DAOQOC010000473 DAOQOC010000000
VERSION     DAOQOC010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31025_length_2217_cov_3.364477"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="dcm"
                     /locus_tag="PL106_08235"
     CDS             complement(<1..640)
                     /gene="dcm"
                     /locus_tag="PL106_08235"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNA33096.1"
                     /translation="MGSLRVAELFAGVGGFRIGLERAGAFQVVWSNQWEPGRRKQHAS
                     EVYTARFGTEHHVCSDIATVPTSDIPDHDLLVGGFPCQDYSVASTLKNAKGLVGRKGV
                     LWWQIHRILDQKKHKPRWLLLENVDRLLGSPAGQRGRDFAVMLASLNALGYTVEWRVI
                     NAAEHGMPQRRRRVFIFGERGVRMPGPRFDAHEYLLKEGLFACAFPRAEAVGD"
     gene            complement(812..884)
                     /locus_tag="PL106_08240"
     tRNA            complement(812..884)
                     /locus_tag="PL106_08240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(849..851),aa:Gly,seq:ccc)
     gene            1192..1755
                     /locus_tag="PL106_08245"
     CDS             1192..1755
                     /locus_tag="PL106_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33097.1"
                     /translation="MRMFQVILTALVAQGIAAQTAPTGSTSHAAYVVTEEISLAMNKA
                     QIIQALPDAWGVSFAQEPGAHLGPVDVEGGIAEGQARVNYRSKLLTGREETMGTITYR
                     VTIQAHNGRCIVRVHDLRHTGNRNAIGGGIHAGPILEGTAPDEGYPGMGLGSSRRIHA
                     DIRGAADARIRETIRRFAGRLRTLAGE"
     gene            1823..>2217
                     /locus_tag="PL106_08250"
     CDS             1823..>2217
                     /locus_tag="PL106_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33098.1"
                     /translation="MNASNWRVWPLIAGSLALLFIAWDIALMSRTRAVQDRISRQVDL
                     LEGLSGIKSDLNQLALTHRVDVNTGQSNWGRSLELARQRVEVTSLRYANEPGMAGLPD
                     LLAIQLRACDSLHKDMKEVGDTARYSHQR"
BASE COUNT          419 a          713 c          692 g          393 t
ORIGIN      
        1 tgtcacccac ggcttccgca cgcgggaatg cgcaggcgaa caagccctcc ttcaggaggt
       61 attcgtgggc atcaaagcgc gggcccggca ttcggacgcc gcgctcaccg aagatgaaga
      121 ccctccttcg ccgttggggc atgccatgtt cggcggcatt gatcacgcgc cactccacgg
      181 tgtaccccaa tgcattcaac gaagcgagca tcacggcgaa atcgcgcccg cgctggcctg
      241 cgggcgagcc cagcaaccgg tccacgttct ccagcagcaa ccagcggggt ttgtgcttct
      301 tttggtcaag gatccgatgg atctgccacc acagcacgcc cttcctgccc accaggcctt
      361 tggcgttctt cagggtgctg gccacggaat agtcctggca ggggaagccg cccaccagca
      421 ggtcgtggtc cggaatatcg gaggtgggca cggtagcgat atcggaacat acatgatgtt
      481 cggtaccgaa tcgggccgtg tacacctcgc tggcgtgctg cttccggcgt cccggctccc
      541 actggttgct ccagaccacc tggaaagcgc ccgcccgttc cagcccgata cggaagccgc
      601 ccaccccggc gaacaattcg gccacgcgca atgatcccat ggcggcctgc ttgcgggcgg
      661 caggcttctt tttcgactgc ttcttcaaag cgtggtgtgc gggggccaat ataggatcgg
      721 gccaccagcc atggccggat gcggccatgg ccaggtggcg acccgatgga acggcaagag
      781 gcgatcatcg cggaccgcct cttgcctcat gagcgggaaa cgggattcga acccgcgacc
      841 ctcagcttgg gaagctgatg ctctaccaac tgagctactc ccgcgcttgt accttgccct
      901 gaccggacgt acctgttctg atccttccgg tgaaggcgcg gcggcgaagg tagcagcccg
      961 gtgtgatccg ttgggcggcg atgggatcgg gtcccgtcgt gaaccccgat agttgaggtc
     1021 caccggcttt gggcgcagga gtccgttcca tccgtaacgc gaagctggcc acctccggac
     1081 atcgctgcgg aactggcgca ctccttgcag ttcaccaccg ttcaccacca tccagcacca
     1141 tcggaccgga ccgacgttcc accccgcttt tcgcccacta acttgcccgc cgtgcgcatg
     1201 ttccaggtca ttcttaccgc acttgtggcc caggggatcg ccgctcagac cgcgccgacg
     1261 ggatccacct cgcacgcggc ctatgtggtg actgaagaga tcagcctggc catgaacaag
     1321 gcgcagatca ttcaggcatt gcccgatgca tggggcgtca gtttcgcaca agagcccgga
     1381 gcgcacttgg ggccggtgga tgtggagggc ggcatagccg aagggcaggc acgtgtgaac
     1441 taccggagca aattgctcac agggcgcgaa gagaccatgg gaacgatcac ctaccgggtg
     1501 accatccaag cccataatgg ccgatgcatc gtacgtgtgc atgatctgcg gcatacgggc
     1561 aaccggaatg cgatcggcgg tggcatacac gctggcccca tcctggaagg aactgctccg
     1621 gacgaaggat atcccggcat gggtctgggt tcatcgcggc ggatacatgc ggacatccgt
     1681 ggcgcggccg atgcccggat ccgggagacc atccgccggt tcgccgggcg cctacggacc
     1741 ctggcgggtg aataaacccc gccggtcgaa acctgccgcg gttttggccg tttgaaatgg
     1801 gcacaccacg ttgcggatgc ccatgaatgc ctcaaactgg cgcgtttggc cgctcattgc
     1861 cggttcactc gccctgctgt tcatcgcttg ggacatcgcg ctgatgtcgc gcacacgcgc
     1921 ggtacaggac cggatcagtc ggcaagtgga cctgctcgag ggcttgagcg gcatcaagag
     1981 cgacctgaac caactggcgc ttacccaccg cgtggacgtg aacaccggcc agagcaactg
     2041 gggccggtcg cttgagctgg cgcgccaacg cgtcgaagtg accagtttgc gatacgccaa
     2101 cgaacccggc atggcgggac tgcccgacct gctcgcgatc caattgaggg cgtgcgattc
     2161 cttgcacaag gacatgaagg aggtcggtga cacggcccgg tactcacatc agcggat
//
LOCUS       DAOQOC010000474         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31029_length_2217_cov_3.151711, whole genome shotgun sequence.
ACCESSION   DAOQOC010000474 DAOQOC010000000
VERSION     DAOQOC010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31029_length_2217_cov_3.151711"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PL106_08255"
     CDS             complement(<1..252)
                     /locus_tag="PL106_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33099.1"
                     /translation="MPGVHMHEGRIGVPLITLERTESTNKYAAELLSQGKVAHGAVIV
                     AHEQTAGRGQRGRIWHSQAGADLAMSVVLGYKRLEAGAQF"
     gene            340..714
                     /gene="rsfS"
                     /locus_tag="PL106_08260"
     CDS             340..714
                     /gene="rsfS"
                     /locus_tag="PL106_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNA33100.1"
                     /translation="MKKNPRLATSSHVDAVVNGIQEVKGRDIVHLDLRAVPNTVCDHF
                     IICHGDSDTQVEAIAGSVEKVVRERIGERPWHIEGMENRGWVVLDYIDVVVHIFHRRL
                     RDHYGLEQLWSDAIRNTYENVA"
     gene            730..>2217
                     /gene="ftsH"
                     /locus_tag="PL106_08265"
     CDS             730..>2217
                     /gene="ftsH"
                     /locus_tag="PL106_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNA33101.1"
                     /translation="MENQQQGNKRKGPEKGINFYWIYGLIGVLILSMWLFNMGPRTQR
                     IDMSEYERFLDAGDVARITIVQNEGVQVTIKADSLAKKVHQARLSRSGADKDKSGPHY
                     DFYTGTSDNLQDQLIAEAKAHHVSVDIRPANNMAAEIWQYLFLAGMVVLLWVFMMRRI
                     GGPGGPGGQLFNIGKSRAQVFEGGKSTNVNFNDVAGLEEAKEELQEIVDFLKNPKRYT
                     DLGAKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSLSGSDFVEMFVGVGASRVRDL
                     FKQAKEKAPSIIFIDEIDAIGRARGRSVSMGANDERENTLNQLLTEMDGFGTNSGVIL
                     LGATNRADVLDRALMRPGRFDRQIFVDMPDLNERVAILKVHLKPLKLDDAVDVEFLAR
                     QTPGFSGADLANICNEAALIAARKKRKQVEKQDFLDAVDRVVGGLEKKNKIITTEEKK
                     AIAYHEAGHATVSWLVEHASPLVKVTIVPRGRSLGAAWYLPDERHL"
BASE COUNT          460 a          684 c          679 g          394 t
ORIGIN      
        1 gaattgtgcc ccggcctcca agcgcttgta tccgagcacc acgctcatcg cgaggtccgc
       61 acctgcttgg ctgtgccaga tccgcccgcg ctgcccacgc ccggccgttt gctcatgcgc
      121 cactatgacc gctccatgcg ccaccttgcc ttgcgacaac agttcggcag cgtacttgtt
      181 ggtgctttcc gtgcgctcca gcgtgatcaa tggcaccccg atccggccct catgcatgtg
      241 tacaccgggc acgtcgtcgg taccttcccc ccgttcaccc tttccgccgg aacttcgcga
      301 ccgcatcctg cttacttttg atcggaacaa agtaaccgaa tgaagaaaaa cccccgcctc
      361 gccacttcaa gtcacgtgga cgccgtcgtg aacggcatcc aagaggtgaa agggagggat
      421 atcgttcact tggacctgcg tgcggtgccc aacaccgttt gcgaccactt catcatctgc
      481 cacggcgatt cggacacgca ggtggaggcc atcgcgggca gcgtggaaaa ggtggtgcgc
      541 gaacgcatcg gcgagcgacc gtggcacatc gaaggcatgg agaacagggg gtgggtggtg
      601 ctggattaca tcgatgtggt ggtccacatc ttccatcggc gcttgcgcga tcattacggg
      661 ttggagcagc tatggagtga cgccatccgg aacacatacg agaacgtggc ctgaagaagg
      721 catttgaacg tggagaacca gcaacagggg aacaagcgca aagggcctga aaaaggcatc
      781 aacttctatt ggatctacgg cctgattggc gtattgatcc tctcgatgtg gttgttcaac
      841 atgggcccca ggacccagcg catcgacatg agcgagtacg agcggttcct ggacgccggc
      901 gatgtggccc gtatcaccat cgttcagaac gagggcgttc aggtgaccat caaagccgac
      961 agcctggcca aaaaagtgca tcaggcgcgc ctctcgcgtt cgggcgccga caaggacaag
     1021 agcggtccgc actacgattt ttataccgga acatcggaca acctccagga ccagttgatc
     1081 gccgaggcca aagcgcatca tgtttccgtg gacatccgtc cggccaacaa catggcggcg
     1141 gagatctggc aatacctgtt cctggccggc atggtggtgc tcctgtgggt cttcatgatg
     1201 cgccggatcg gtggcccggg cggcccgggc ggtcagctgt tcaacatcgg taagagccgg
     1261 gcgcaggtct tcgagggggg caagagcacc aatgtcaatt tcaatgatgt ggccggcctg
     1321 gaagaggcca aggaggaatt gcaggagatc gtcgatttcc tcaagaaccc caagcgctac
     1381 accgatctgg gcgccaagat ccccaaaggg gcgctgctcg tggggcctcc cggaacagga
     1441 aaaacactcc tggcgaaggc cgtggccggg gaggccaatg tgcccttctt cagcctgagc
     1501 ggttccgact tcgtggagat gttcgtgggc gttggcgcca gccgcgtgcg cgacctcttc
     1561 aaacaggcca aggagaaagc gcccagtatc attttcatcg acgagatcga tgcgatcggc
     1621 cgtgcgcgcg gacgcagcgt gagcatgggc gccaacgacg agcgcgagaa caccctcaac
     1681 cagctcctca ccgagatgga cggcttcggc accaacagcg gcgtgatcct gctgggcgcc
     1741 accaaccgcg ccgacgtgct ggatcgtgcc ctcatgcgcc ccggccgctt cgacaggcag
     1801 atcttcgtgg acatgcccga cctcaacgag cgcgtggcga tcctcaaggt acacctcaag
     1861 cccctgaagc tcgatgatgc ggtggacgtg gagttcctcg ctcgccagac ccccggcttc
     1921 tccggcgccg acctcgccaa tatctgcaac gaggccgcgc tcatcgccgc gcgcaagaag
     1981 cgcaagcagg tggaaaagca ggatttcctc gatgcggtgg atcgcgtggt gggtggcctg
     2041 gagaagaaga acaagatcat caccaccgag gagaaaaaag ccattgccta ccacgaggcc
     2101 ggccacgcta cggtgagctg gctggtggag cacgcgagcc ccttggtgaa ggtgaccatc
     2161 gtgccgcgtg gccgatcgct cggcgccgcc tggtacctcc ccgatgagcg tcacctc
//
LOCUS       DAOQOC010000475         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31040_length_2217_cov_2.645698, whole genome shotgun sequence.
ACCESSION   DAOQOC010000475 DAOQOC010000000
VERSION     DAOQOC010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31040_length_2217_cov_2.645698"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PL106_08270"
     CDS             <1..234
                     /locus_tag="PL106_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33102.1"
                     /translation="GMISAIKTFFERQSFGVCAWWGEKLNIKTEQVRLSFIYLACITA
                     GLHLVFYLVMAFVLEHKERIKRPFAKRSTVWEF"
     gene            complement(306..1511)
                     /locus_tag="PL106_08275"
     CDS             complement(306..1511)
                     /locus_tag="PL106_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33103.1"
                     /translation="MRQLLTLASLVLPFAIQAQWTPMTVGTMSNRSLSEQGGDLYCAS
                     FPAGIKKSIGGTGTWTAVNTGLPSNGSNYYVQSVGTDGTNLYAGTESGVYRSTNNGTS
                     WTNINGTLAASNTVYANKFFSTGTNFVLAIYEGTIAQGGGIWRTLDNGANWLIGHSGM
                     TSNAKVNNVTKVGNTLWASTSVGLFTSTDNAQNWTAHPTVNYAVFGLASGNGTLVIAS
                     TFGMRYSTNNGTSWQDATGDPASPTDGEVVFFDGRFFTVLNGVGCLRSENNGAAWTDH
                     NTGFGPVDASAQEEFLTTTNTLYCTALFDVYSLAATNTAVSTITDPHGIILFPTAFDE
                     VLTLRGVTRESDLLLIDGAGRTCLTQRIVPGGDQHIDRNGLAAGTYRALLIDRRSGQQ
                     TLLGAVVAR"
     gene            complement(1575..1937)
                     /locus_tag="PL106_08280"
     CDS             complement(1575..1937)
                     /locus_tag="PL106_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33104.1"
                     /translation="MRTLRITGLVVLLSLFGTTARAQADGPAPSSLMNDLCGCMGAID
                     LRAGDRTVEQGVRDCLEEAVLRYPSEVYAVLQHTPEGGSKAFRLGTALGGHLLRVCEP
                     FHAVKARLQQMPLRKQGT"
BASE COUNT          389 a          668 c          713 g          447 t
ORIGIN      
        1 ggtatgatct ccgccatcaa gaccttcttt gagcgccagt ccttcggcgt gtgcgcttgg
       61 tggggcgaaa agctcaacat caagaccgag caggtccggc tcagcttcat ctatctggcg
      121 tgcattaccg cagggctgca cctggtcttc tacctcgtca tggccttcgt gctcgaacac
      181 aaggagcgca tcaaacgacc attcgcgaag cgaagcaccg tttgggagtt ttgacgagga
      241 cgtggtacgc cttctcccgg gatcgtgcat gccggcacga tcgcaacaag aggatcggcc
      301 cgcactcacc gggccacaac cgcgcccagc agggtctgct gaccgcttcg acgatcgatc
      361 agcagggcac ggtaggtccc tgcggcaagt ccgttccgat cgatgtgctg atcaccgccc
      421 ggcacgatgc gctgcgtgag gcaggtgcgc cctgcgccat cgatcagcag caggtcgctc
      481 tcccgcgtga cgccgcgcaa ggtcaaaacc tcgtcgaatg cggtcgggaa caggatgatt
      541 ccgtggggat ccgtgatcgt ggacacggcc gtattggtcg ctgccaaact gtacacatcg
      601 aacagcgcgg tgcagtacag cgtgttcgtg gtcgtcagga actcctcctg tgcactggcg
      661 tccaccgggc cgaaaccggt gttgtgatcg gtccacgcgg cgccgttgtt ctcgctgcgc
      721 agacatccga caccattgag cacggtgaag aacctgccgt caaaaaatac aacctcgcca
      781 tcggtagggg atgcggggtc gcccgtggcg tcctgccagc tggtgccgtt attggtgctg
      841 tagcgcatgc cgaaggtgct cgcgatcacc agggtgccgt tgcccgaagc cagcccgaat
      901 accgcatagt tcaccgtagg atgcgccgtc cagttctgcg cgttgtccgt gctggtgaac
      961 agtccgacac tggtgctggc ccacaaggtg ttgcccacct tggtcacgtt gttcaccttg
     1021 gcgttgctgg tcatgccgct gtggccgatg agccagttgg ccccgttgtc caaggtgcgc
     1081 cagatgccac cgccttgcgc gatggtaccc tcgtagatgg ccagaacgaa attggtacct
     1141 gtggagaaga acttgttggc atataccgta ttgctcgcgg cgagcgtacc gttgatgttg
     1201 gtccaactgg tgccgttgtt cgtgctgcgg tagacaccgc tctccgtgcc cgcgtacagg
     1261 ttggtgccgt ccgtacccac gctctgcacg taatagttgg agccgttcga tggcaatcct
     1321 gtgttcaccg cggtccacgt accggtcccg ccgatgcttt ttttgatgcc tgcgggaaag
     1381 gaggcacaat acaggtcgcc accctgctca ctgaggctgc ggttggacat ggtgccgacg
     1441 gtcatcgggg tccattgggc ctggatggcg aaggggagaa cgagtgaagc gagcgtaagg
     1501 agttgacgca tgggcaagtt gttcggggcc gcgaaaatag ggcaggggcc gtgtggcggt
     1561 gtgtgccggg aggctcaggt gccctgcttc ctgagcggca tctgctggag ccgggctttt
     1621 accgcatgga agggttcaca gacccggagc aggtggccac ccagagcggt accgagccgg
     1681 aaggctttgg atccgccctc cggggtgtgc tgcaggaccg cgtacacctc cgacggatag
     1741 cgaagtacgg cctcctcgag acaatcgcgc acaccctgtt ccacggtgcg gtccccggcc
     1801 cgcagatcga tggcgcccat gcacccgcac aggtcgttca tgagggagga tggggccggg
     1861 ccgtccgctt gggcccgtgc ggtggtaccg aacaggctca gcagtacaac aagaccggtg
     1921 atcctcagcg tcctcatccg gaagggagcg tgtgcaagtt aacgcgtcga acggtattga
     1981 attgccgatg tgatgaccgg ttatcaaccg tgatggcgcg atcggcctgg acggcccggt
     2041 gatccatccg gtgttacgaa cctgcctggt cggcattgat gggcaggtac cggcctcatg
     2101 ctccgttccc cactcatcct ctgcctgttt cttccgctgt tggcgcgatg ccagggtgga
     2161 actggttccg cgcctcagga gccgaccgtc cggaagtcgc cgccggtgcg tcccctg
//
LOCUS       DAOQOC010000476         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31043_length_2217_cov_2.451896, whole genome shotgun sequence.
ACCESSION   DAOQOC010000476 DAOQOC010000000
VERSION     DAOQOC010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31043_length_2217_cov_2.451896"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PL106_08285"
     CDS             complement(<1..1375)
                     /locus_tag="PL106_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33105.1"
                     /translation="MKANTPFRTALAATLAFHLMAPVAKAMDYNTAHGIHMPPAHGTP
                     GTLKAAACTPAVAITELAYNNVRALITTSGLLWYDKANNKPAYEVPKTVDRTGAKAIF
                     SGGLWMGGLSPDNQLKIAAIQYRNGNDFWPGPLTTGGDASVTSEVCSLYDRFYTTERL
                     DAERHRAYYECLSNPQECNVATEFPDGYITPTVFFDWPAINNTPGFDTYLAPFTDVDG
                     NSDYDPNSGDYPGYDLERVIDCNTRNPNDPVPLFGDHNIWWVMNDKGNQHTETGGQPI
                     GLEIRSQAFAFATNDEINNMTFYNYLVINRGTQTLTNAYFGQYADPDLGFADDDFTGC
                     DVQRGLGYVYNGDENDEGGNGNPGYGMQPPAIGIDFFEGPYQDPDGVDNPGPPDTSEP
                     FPDCGQAVAQHGIGYKGIGIGYGDNVADNERFGMRAFLYFNREGPACCNDPGLAVHYY
                     NYLRSIWK"
     gene            complement(1409..>2217)
                     /locus_tag="PL106_08290"
     CDS             complement(1409..>2217)
                     /locus_tag="PL106_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33106.1"
                     /translation="RDQIQIRNVAEAWPVTYKTYDNYDFGTVKGLSVAYDMRRTGNIR
                     LTATYTLQFADGTGSDPNTSLSLLNAGQPNLRTIAPLNFDQRHRITTNVDFSYGNGND
                     YNGPVIAGAKILQNTGVNFTTILGSGTPYSISSLVVQGGNMNPPGGRLVGTINGARLP
                     WQFNTDLQLYRDIPLSFGKDGDADKAKKANLNVYLLVTNLFNNKVITGVYRATGSPTD
                     DGYLAAARYQDNINNQIDPQSFRDLYALKVNNPYNFGAPRTIRLGLRFDF"
BASE COUNT          392 a          604 c          741 g          480 t
ORIGIN      
        1 ccttccagat gctccggaga tagttgtaat agtgtaccgc aagacccgga tcgttgcagc
       61 aggcgggtcc ttcccggttg aagtagagga aggcacgcat accgaagcgc tcgttgtcgg
      121 ccacattgtc accgtatccg ataccgatgc ccttgtaacc gatcccatgc tgcgccacgg
      181 cctggccgca atcggggaaa ggttcgctcg tgtcgggcgg gccgggattg tcaacgccat
      241 ccggatcctg gtagggccct tcgaagaaat cgatgccgat ggcgggcggt tgcatcccat
      301 agcccgggtt gccgttacca ccttcgtcgt tctcatcccc gttgtacacg tagcccaacc
      361 cacgctgcac gtcgcagccc gtgaagtcgt cgtccgcgaa gcccaggtcg ggatcggcgt
      421 actggccgaa ataggcgttg gtcagggtct gcgtaccacg gttgatgacc aggtagttgt
      481 agaaggtcat gttgttgatc tcatcgttgg tggcgaaggc gaaggcctgc gaacggatct
      541 ccaatccgat gggctgtcca ccggtctcgg tgtgctggtt gcccttgtcg ttcatcaccc
      601 accagatgtt gtgatcgccg aagaggggca ccggatcgtt cgggttgcgg gtgttgcaat
      661 cgatcacgcg ctccaggtcg tagcccgggt aatcgcccga attggggtcg taatcactat
      721 tcccgtccac atcggtgaaa ggggccagat aggtatcaaa accgggcgtg ttgttgatgg
      781 ccggccagtc aaagaagacg gtgggggtga tgtagccatc cgggaactca gttgccacgt
      841 tgcattcctg cgggttgctc aggcactcgt aataggcgcg gtgccgttcg gcatccaacc
      901 gttctgtggt gtagaagcgg tcgtacaggc tgcatacctc gctggtcacc gaagcatcgc
      961 cgccggtggt gaggggcccg ggccagaaat cgttaccgtt acggtattgg atcgccgcga
     1021 tcttcaactg gttgtccggt gagaggccgc ccatccacag accacccgag aagatggcct
     1081 tggcaccggt acgatcaacg gtcttgggca cctcgtacgc cggcttgttg ttggccttgt
     1141 cgtaccagag caggccactg gtggtgatca gggcacgcac gttgttgtag gccagctcgg
     1201 tgatggccac cgcaggggta caggcagcgg ctttcagggt accgggggtg ccgtgggccg
     1261 gtggcatgtg gataccgtgg gccgtattgt agtccatggc cttggccacg ggcgccatca
     1321 gatggaacgc gagcgtggcg gcaagggcgg tgcggaacgg ggtgttcgct ttcatacttt
     1381 gaacgccgaa gcccggcgca ggcgttgttc agaaatcgaa gcgcaagccc aagcggatgg
     1441 tgcgtggcgc tccgaagttg taaggattgt tgactttcag ggcgtagaga tccctgaagc
     1501 tctgcgggtc gatctggttg ttgatgttgt cctggtagcg cgcggcggcc aggtagccat
     1561 catccgtggg cgaaccggtg gcgcggtaca cgccggtgat caccttgttg ttgaagaggt
     1621 tcgtgaccag gaggtacacg ttcagattgg ccttcttggc cttgtcggca tccccgtcct
     1681 tgccgaagct gagcgggatg tcgcggtaca actgcaggtc ggtgttgaac tgccagggca
     1741 ggcgcgcacc attgatcgtg cccaccaggc ggccaccagg cggattcatg ttgcccccct
     1801 gcaccaccag actggagatg ctgtacgggg tgccgctgcc gaggatggtg gtgaagttga
     1861 caccggtgtt ctgcaggatc ttggcacccg cgatgaccgg gccgttgtaa tcgttgccat
     1921 tgccatagct gaagtccaca ttggtggtga tgcggtggcg ctgatcgaag ttcagcggtg
     1981 caatggtgcg caggttgggt tggccggcat tcaggagcga gaggctggtg ttgggatccg
     2041 aaccggtacc atcggcgaat tgcagggtgt acgtcgcggt gaggcggata ttgccggtgc
     2101 gcctcatgtc gtacgctacg ctcaaaccct tcaccgtacc gaaatcatag ttgtcgtagg
     2161 tcttgtaggt caccggccag gcctcggcca cgttccggat ctggatctgg tcgcgca
//
LOCUS       DAOQOC010000477         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31171_length_2212_cov_3.201205, whole genome shotgun sequence.
ACCESSION   DAOQOC010000477 DAOQOC010000000
VERSION     DAOQOC010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31171_length_2212_cov_3.201205"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PL106_08295"
     CDS             complement(<1..374)
                     /locus_tag="PL106_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HNA33107.1"
                     /translation="MKKISDSRKLLGATSTTTLKELNTLYKGLMKQHHPDRFQDEQLR
                     QKAEVTSQHVIECYKFLESIHPETHAANAAEFEKTVASNIGNWHYQHMTLHITFGDGS
                     AYEFYSVDPKTYNKFVNTDGNTR"
     gene            489..1502
                     /locus_tag="PL106_08300"
     CDS             489..1502
                     /locus_tag="PL106_08300"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNA33108.1"
                     /translation="MERYLAFLPAVCCSFVVSAQLTVTTQLSEVQVTDLLEGLGVTVT
                     NVDINCPGDALGHFMGASELGINQGIVLTSGSAAAVAGPSSSFASSGAMTAGDEDLEL
                     WVSQPTFDACALEFDCIPIGDTLEFNFAFGSEEYPEFAFSAFNDVFGIFMSGPGITGP
                     YSNNAENIAWVPGTNVPVSINNVNEAENSVFYHNNEDPPGQYLAYDGFTSNLPVFATV
                     QPGESYHFKVVVADVSDGVFDSGVVLEAFSFHSTAASVGVAEAQRPVWSLAQDADEVF
                     VIWPENMGVRRVEVLDATGTVVEQRVSNGNRVVIHTGTLAAGMYFVRLQDGTVPPLRF
                     VKR"
     gene            complement(1560..1694)
                     /locus_tag="PL106_08305"
     CDS             complement(1560..1694)
                     /locus_tag="PL106_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33109.1"
                     /translation="MPTSALTRRQPRNTRITGGRDWTILMALFLGAIGLAMILLLTWS
                     "
     gene            1908..>2212
                     /locus_tag="PL106_08310"
     CDS             1908..>2212
                     /locus_tag="PL106_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNA33110.1"
                     /translation="MKPSIPKGTRDFGPQEMLRRKYIFNTMEQVFQRYGFLPLETPAM
                     ENLETLTGKYGEEGDRLIFKILNSGDAWKEVAPEVKGKLMGGEEVHPGKLAAELSEKA
                     "
BASE COUNT          414 a          615 c          717 g          466 t
ORIGIN      
        1 cgcgtgttgc cgtccgtgtt cacgaacttg ttgtaggtct tcgggtccac gctgtagaac
       61 tcgtacgcac ttccgtcgcc gaaggtgatg tgcagcgtca tgtgctggta gtgccagttg
      121 ccgatgttgc tggccacggt tttttcgaac tccgccgcat tggccgcatg ggtttccggg
      181 tggatgctct cgaggaactt gtagcattcg atcacgtgct ggctggtgac ctcagccttc
      241 tgcctgagtt gctcgtcctg gaagcggtcc ggatggtgct gcttcatcag gcccttgtac
      301 agtgtgttca gctctttcaa ggtggtggtg ctcgtggcgc ccaagagctt gcggctgtcg
      361 gagatcttct tcatgcggcg aaagggccgc gaagatgcgg tattgaggga caggtcggtc
      421 ccgtccgggc tggttgatcg ggtcgaatac cggtggatca ctacctttgg gacacaaacc
      481 cccaccacat ggaacgatac ctcgcgtttc ttcccgctgt atgctgctcc ttcgtcgtga
      541 gtgcgcaact aacggtgacg acgcaactct ccgaggtcca ggtgacggat ctgttggagg
      601 gcctgggtgt cacggtgacc aacgtggata tcaattgccc gggcgatgcg ctggggcatt
      661 tcatgggagc cagtgaattg gggatcaacc aaggcattgt actgacctcg ggctccgcgg
      721 ccgctgtcgc tggtccgagt tcctccttcg catcgagcgg ggccatgacc gcgggcgacg
      781 aggatcttga gttgtgggtc tctcaaccga ccttcgatgc ctgcgcgctg gagttcgact
      841 gtatcccgat cggcgataca ctcgagttca atttcgcttt cgggtccgaa gaatatcccg
      901 agttcgcctt ctccgcattc aatgatgtct tcggcatctt catgagcggt cccgggatca
      961 ccgggccgta cagcaacaac gcggagaaca tcgcgtgggt gccgggcacg aacgtgcccg
     1021 tgtccatcaa caacgtgaat gaagctgaga attcggtgtt ctaccacaac aacgaggatc
     1081 cgccgggcca gtatctcgcg tacgacggct tcacctcgaa cctgcccgtc ttcgccaccg
     1141 tccagcccgg ggagtcgtac catttcaagg tcgtggtggc ggatgtttcc gatggggtgt
     1201 tcgacagtgg cgtggtgctc gaggccttca gcttccacag taccgcggcc agtgttggtg
     1261 tcgccgaagc gcagcggccg gtgtggtcgc ttgcacagga tgccgatgaa gtgttcgtga
     1321 tctggccgga gaacatgggt gtgcggcgcg tggaggtgct cgatgcgacc ggtacggtgg
     1381 ttgaacaacg cgtgagcaat gggaaccggg tggtcatcca cacgggaaca ctggcagcag
     1441 ggatgtactt cgtgcgcctc caagatggca cagtaccacc cctccgcttc gtgaaacgat
     1501 agatcaccgc ccctgaagtg gtcgatcagg ggagcacagc aggccagtcc ggtatcggtt
     1561 caggaccagg tcagcagcag gatcatggcc agtcctatgg cgcccaagaa gagggccatc
     1621 aggatggtcc agtcccggcc gccagtgatc cgtgtgttgc gcggctggcg tcgggtgagg
     1681 gctgaagtgg gcacgtggat cctttagggg cgcaaaattc tgaggatcgg ggtgcgcagc
     1741 gccagcggct ttcgacaacc gatgagcccg gctcgtcaaa tggtgttcct gcaacgccaa
     1801 gccatggggt cggcccgaca ggatgggaca tgggcgatac tttcgcgccc ccaccgccag
     1861 ggctgccttg gagctttggc cttggaactt gagtcttgaa ccttgcaatg aaaccctcga
     1921 tccccaaagg cacccgcgac ttcggccccc aggagatgct gcgccgcaag tacatcttca
     1981 acaccatgga gcaggtattc cagcgatacg gctttctccc cttggaaaca cccgcgatgg
     2041 agaacctgga aacgctcact ggcaaatacg gggaggaggg cgaccgcctg atcttcaaga
     2101 tcctcaacag tggggatgcg tggaaggagg tggcgccgga agtgaagggg aagctcatgg
     2161 ggggagagga agtgcatccc ggtaagctgg cggccgaact gagtgagaag gc
//
LOCUS       DAOQOC010000478         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31329_length_2206_cov_2.894933, whole genome shotgun sequence.
ACCESSION   DAOQOC010000478 DAOQOC010000000
VERSION     DAOQOC010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31329_length_2206_cov_2.894933"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PL106_08315"
     CDS             <1..541
                     /locus_tag="PL106_08315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA33111.1"
                     /translation="SRGGKWMVLRNTIIIFLVSGFWHGANWTFIAWGAINAAFFIPLI
                     LTGANRRFTGPIAEGRILPTRDELLRMLVTFALATFTRVFFRAENMTDAMEVFKKVFS
                     ASLFSPPQFTHVRDLVFSVSGILIMLVIEWINRDRQHGLQLDGRATRPVRYTLYYGLI
                     ALMILMAPLGGGEFIYFQF"
     gene            548..1498
                     /locus_tag="PL106_08320"
     CDS             548..1498
                     /locus_tag="PL106_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33112.1"
                     /translation="MPFRFALRCLKYSLPVVVMLAVYCGFYARYFPAPRVTNNVAVNE
                     KFAAFARQDWSATNHPDVLAFGSSMALNNLSSDEVVARLGAKRYFNLGAWGLDMSQTC
                     TLAHAFAERYHPRTIVIAGNLRDFSLSLERSKMRLGPVLELVEKGPGLVSYLTHPEFV
                     YYLRQMESNRLRFTDRANYECLLMDAHGGVALDVPKERILAERWVRPVPATDDLAQKH
                     YDALDRLASGLRAMGIDLIYVTPPYRDGIRTPEAIDAINRHIAKVRGLITARGQYYCD
                     GYDRSWPDDLYADSSHLNGPGARLFTAHALARLKPSMASR"
     gene            1538..>2206
                     /locus_tag="PL106_08325"
     CDS             1538..>2206
                     /locus_tag="PL106_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNA33113.1"
                     /translation="MRMPIDGKPIKGRGAAVQVANRFLKHSYGVVHWEGIDEVEEEDR
                     RTRYVVEHPRTIVNKVDSPDLPFTFSMNPYQGCEHGCAYCYARPTHEYWGYGAGLDFE
                     RVIIVKRSAPEVLRRTLQDRKWTPAPIMFSGNTDCYQPVERKEGITRAMLAVCLEFRQ
                     PVGMITKNALILRDLDILKEMAGLGLVTVAISLTTLNEELRRVLEPRTSTGANRVRVI
                     GELTK"
BASE COUNT          405 a          678 c          676 g          447 t
ORIGIN      
        1 cagccgcggc ggcaagtgga tggtgttgcg caatacgatc atcatcttcc tggtgagcgg
       61 attctggcat ggtgccaact ggaccttcat cgcgtggggc gccatcaacg cggcgttctt
      121 catcccgctc atcctcacag gggccaaccg gcggttcacc gggccgatcg ccgagggccg
      181 aatcctgccc acccgcgatg aactgctccg tatgctcgtc accttcgcac tggccacctt
      241 cacccgggtg ttcttccgtg ccgagaacat gaccgatgcc atggaggtct tcaaaaaggt
      301 cttctccgcc tcgttgttct ccccgcccca atttacccat gttcgcgatc tggtattcag
      361 cgtgtcgggt atcctgatca tgctcgtcat cgaatggatc aaccgggatc gccagcacgg
      421 tctgcagttg gacgggcggg ccacacgccc ggtgcggtac acgctgtact acggcctgat
      481 cgcattgatg atcctgatgg ccccgctggg gggaggtgag ttcatatatt tccagttctg
      541 acccgtgatg cccttccgtt tcgcccttcg atgcttgaaa tacagcctgc ccgtggtggt
      601 gatgcttgcg gtgtattgcg gcttctatgc gcggtatttc ccggcaccac gcgtaacgaa
      661 caacgttgcc gtgaatgaga agttcgccgc gttcgctcgc caggactggt cggccacgaa
      721 ccatcccgac gtgctggctt tcggctcctc gatggccttg aacaacctgt cttccgacga
      781 ggtggtcgca cggctgggcg caaagcggta tttcaacctc ggcgcctggg ggcttgatat
      841 gtcccagacc tgcacgttgg cgcacgcctt cgccgaacgg taccatcccc gcaccatcgt
      901 gatcgcgggc aacctgcgcg atttctccct ctcgctggag cgttcgaaaa tgcgccttgg
      961 ccccgtgctg gaactggttg agaagggacc cgggctggtc tcgtacctga cacatccgga
     1021 gttcgtgtac tacctgcggc agatggagag caaccgcttg cggttcaccg accgtgccaa
     1081 ctacgagtgc ttgctcatgg atgcgcacgg aggtgtcgct cttgatgtgc ccaaggagcg
     1141 gatccttgcg gagcgttggg tgcggcctgt tccggcgacc gacgaccttg cacagaaaca
     1201 ctacgacgcg ctcgaccgcc tggcctccgg gctgcgcgcc atggggatcg acctgatcta
     1261 tgtgacaccg ccttaccgcg atggcatccg cacgcccgag gccattgacg cgatcaatcg
     1321 gcacatcgcc aaagtgcgtg gactgatcac cgcacgtggc cagtactatt gcgacgggta
     1381 cgaccgttcc tggccggatg acctgtacgc ggacagcagc catttgaacg gtcctggagc
     1441 aaggctgttc accgcgcacg ccctcgcgcg cctgaagccg agcatggcgt cgcgctgaag
     1501 ccgccaagct cggcattcca ctgtcctgcg ctacattatg cgcatgccga tcgacgggaa
     1561 acccatcaag gggcgtggtg cggccgtgca agtggccaac cgattcctga agcacagcta
     1621 tggtgtggtt cattgggaag ggatcgacga agtggaggag gaggatcggc gcacgcggta
     1681 cgtggtggag catccgagga cgatcgtcaa caaggtggac tccccggatc tgccattcac
     1741 cttcagtatg aatccgtacc aaggatgtga gcatggctgt gcctactgtt acgcccggcc
     1801 cacgcacgag tactggggct acggtgcggg cctcgatttc gaacgggtga tcatcgtcaa
     1861 gcgcagcgcg cccgaggtgt tgcggcgcac ccttcaggac cgcaagtgga cccctgcacc
     1921 catcatgttc agcggcaaca ccgattgcta ccagcccgtg gagcgcaagg agggcatcac
     1981 gcgggccatg ctcgccgtat gcctggagtt ccgccagccc gtgggcatga tcaccaagaa
     2041 tgcattgatc ctgcgtgatc tggatatttt gaaggaaatg gcgggcctgg gactggtcac
     2101 cgtggccatc agcctaacca cgctgaacga ggagctgcgg agggtattgg agccgcgtac
     2161 cagcaccgga gcgaaccgtg tgcgtgtgat cggagaattg acgaag
//
LOCUS       DAOQOC010000479         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31385_length_2204_cov_2.763611, whole genome shotgun sequence.
ACCESSION   DAOQOC010000479 DAOQOC010000000
VERSION     DAOQOC010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31385_length_2204_cov_2.763611"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PL106_08330"
     CDS             <1..606
                     /locus_tag="PL106_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33114.1"
                     /translation="IALGAEPMIIAVPEAWGVLASDHGSRCYPHRVQGEGFFIALLRK
                     PGERTPSGSGMGPCDPPAPALSSWLKHPELLTTKELHGIHYASSRRWCVVIDRIERTI
                     PVLASGLPVAEEKGRTIRPHAALALNEELDTAAFPSVDLELPRALAYLRGETALSLPA
                     HSDPGHGTLLVRYQGLPIGWMYRAGDRWNNGWPKPWRIRMR"
     gene            640..1386
                     /locus_tag="PL106_08335"
     CDS             640..1386
                     /locus_tag="PL106_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33115.1"
                     /translation="MFGRFLDWFERHKFGVVGTLMLHTFMLFGMALSKLSPGNEAAAH
                     EDMVMELAAPEELPPDPMLQPPLSAPVEVKNTSSNSTAEVRPQMRLYPGPQAQERMAS
                     DIESELREFEAAEFHKQDSARKAQGKEVVMPQLDPSKWDPARYMPRKNEPARVEGLTT
                     VTYDLAGRTDTELDIPAYLCIGQGAVVIAVAVDRDGRVVRAELDEERTHADDCMRESA
                     LRSAQEAHFSASSTAPAPQRGTMTYIFLAQ"
     gene            1552..1995
                     /locus_tag="PL106_08340"
     CDS             1552..1995
                     /locus_tag="PL106_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNA33116.1"
                     /translation="MTLVKHRPQRPFASPFDELFGNLFGRDISHFVGNDDVNTSIPRV
                     NITETKDGFKLDLLAPGFSKEDLKMNVEDDTLTISAEKKSVSLNEGERYTRREFGYSA
                     FKRGFRLPENVKMDSITANYANGVLTVSIAKSEPAKPAVREISIS"
BASE COUNT          467 a          695 c          663 g          379 t
ORIGIN      
        1 atcgccttgg gagcggagcc catgatcata gctgttcccg aggcgtgggg cgtgctggcc
       61 tccgatcacg gatcacgctg ctacccgcac cgcgtgcaag gcgaaggctt cttcatcgcc
      121 ttgttgcgca agcccggcga acgcacgccg tcgggatcag ggatggggcc atgtgatccg
      181 ccggctcctg ctctctcatc ctggttgaaa cacccggaac tgctgacgac gaaggaactg
      241 cacgggatcc actatgcgtc atcgcgccgt tggtgcgttg tgatcgaccg gatcgaacgc
      301 accataccgg ttctggcctc cggtttgccc gtggccgaag aaaaaggtcg aaccatcagg
      361 ccgcatgccg cactggcctt gaacgaagag ctcgacacag ccgcgtttcc ctcggtggac
      421 ctggagctcc cccgcgcgct ggcctacctc cgcggcgaaa cggccctgtc cctgccagcg
      481 cattcggacc cgggccatgg tacgctcctt gtccggtacc aaggtctgcc cataggctgg
      541 atgtaccggg ccggcgatcg ttggaacaac ggttggccga aaccctggcg catccgcatg
      601 cgctgaggtt gcaccggatt actttgcgac aagtacccca tgttcggtcg cttcctcgac
      661 tggttcgaac gccacaaatt cggcgtggtg ggcacgctca tgctgcacac cttcatgctc
      721 ttcggcatgg ccctgagcaa gctgtcgccc gggaatgagg cggccgccca cgaggatatg
      781 gtgatggaac tggccgcacc ggaggagctt ccgcccgatc cgatgctcca gccgccgctt
      841 tcggcccccg tggaggtgaa gaacacatcc agcaacagca ccgccgaggt gcgcccccag
      901 atgcgcctgt acccaggtcc gcaggcgcag gagcgaatgg ccagcgacat cgaatccgaa
      961 ttgcgcgaat tcgaggctgc tgagttccac aagcaggatt cggcccgcaa agcacagggc
     1021 aaggaagtgg tcatgcccca actcgatccc agcaaatggg acccggcccg atacatgccc
     1081 aggaagaacg agcccgcccg ggtggaaggc ctcaccaccg ttacgtacga tctggcgggc
     1141 cgcaccgata ctgaactgga tatacccgcg tacctgtgca tcggccaagg cgcggtagtg
     1201 atcgccgtgg cggtggaccg cgatggccgg gtagtgcgcg cggaactgga tgaggaacgc
     1261 acccatgccg acgactgtat gcgtgagagc gcccttcgtt cggcgcagga agcgcatttc
     1321 agcgcgtcaa gtaccgcccc ggctcctcaa cggggaacca tgacctacat cttcctggcc
     1381 caataggtca tttcgtcggt catcacccgg acgggaatgc cgatccgtca cataattacc
     1441 ttggataacg tacatattgt catgggtcgg tcaaaggcac gtggttcgat gggggcttcg
     1501 caccggtcaa cgtccggcat ccaacacaac acgaaaacaa caccaacaac catgacactc
     1561 gtgaaacatc gcccccagcg tccgttcgcc agcccattcg atgagctttt cggaaacctc
     1621 ttcggtcgcg acatttcgca cttcgtgggc aacgacgatg tgaacacatc gatcccgcgt
     1681 gtgaacatca ccgagaccaa ggatggtttc aaactggacc tgctcgcgcc cggattcagc
     1741 aaggaggacc tcaagatgaa cgtggaggac gacaccctca ccatcagcgc cgagaagaag
     1801 agtgtatcgc tcaatgaagg cgagcggtat acccgccgcg aattcgggta cagcgccttc
     1861 aaacgcggat tccgcctgcc ggagaacgtg aaaatggaca gcatcacggc caactacgcg
     1921 aacggggtgc tcacggtgag catcgcgaag agcgagcctg ccaagccggc ggtgcgcgag
     1981 atcagcatct cgtaaggaga acaccgaacg agggaaaagc ccgggtcgcc cccgggcttt
     2041 tcgcatgata caggaaggcc taccaggctt cgcagaccag cggtgccgag aacggcgggc
     2101 gtgtacggga ccaacttccg atcgaagcat caccgggcag acgtgttcgg gtgggtccct
     2161 cgtagaacca ggtggagttg cctccgccat attgggtatc gatg
//
LOCUS       DAOQOC010000480         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31577_length_2197_cov_2.579832, whole genome shotgun sequence.
ACCESSION   DAOQOC010000480 DAOQOC010000000
VERSION     DAOQOC010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31577_length_2197_cov_2.579832"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PL106_08345"
     CDS             <1..1106
                     /locus_tag="PL106_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HNA33117.1"
                     /translation="KTISNETFVTDLAKMPHLLMAGATGQGKSVGLNAILVSLLYRKH
                     PSQIKFVLVDPKRVELTLYARIERHFLAKLPGAEEAIISDVKKVVPTLNSLCIEMDER
                     YELLKDAQCRNIKEHNAKFIQRRLNPEKGHRYLPYIVLVVDEFADLIMTAGREVETPI
                     ARLAQLARAIGIHLIIATQRPSVNIITGTIKANFPARIAFRVSSKVDSRTILDAGGAD
                     QLIGRGDMLLSTGNDLIRIQCAFVDTPEVEKITDFIGGQRGYPDAMLLPEPPMAEGEG
                     GEDMDDGERDSMFEEAARLVVQTQQGSTSLIQRKLKLGYNRAGRIVDQLESAGILGPF
                     EGSKARRVMIPNEAALAAHLNTGVTTGPGMGGF"
     gene            complement(1414..>2197)
                     /locus_tag="PL106_08350"
     CDS             complement(1414..>2197)
                     /locus_tag="PL106_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33118.1"
                     /translation="RIHLGDLHYRRIKMGEARDAFQRAVDLAPTSTEAKLRLGEVYMV
                     LRDYSKAMELVNEALRIDPNAARGYFLKGYIHMETKDTSRAISSFRTAVEQDPQDYPS
                     YMLLGQLSASRRDRLAEQYYTTAIELRPRSVEAWYGKGLWAQNNGHDSLAIACYDRIK
                     ELDLMNPLPWYNIGFIKLEHLNDPQGAKADFSKAIDLNTNYADAWYSRGIAMERTQQP
                     DSAAANYQICLTIDRTHTLAALAVGRLAKQGVRIKMMERKKK"
BASE COUNT          423 a          682 c          653 g          439 t
ORIGIN      
        1 gcaagaccat cagcaacgag accttcgtaa cggacctggc caagatgccg cacctgctga
       61 tggcgggagc cacgggccag ggcaaatccg ttggtctgaa cgcgatcctc gtttcgctcc
      121 tgtaccggaa gcaccccagc cagatcaagt tcgtcctcgt tgaccccaag cgggtcgaac
      181 tcaccctgta cgcgcggatc gagcgtcact tcctggccaa actgcccggt gctgaagagg
      241 ccatcatctc cgatgtgaaa aaggtggtgc ccacgctcaa cagcctgtgc atcgagatgg
      301 acgagcggta cgagttgctg aaggatgcgc agtgccgcaa catcaaggag cacaatgcca
      361 agttcatcca gcgccgcttg aacccggaga aaggccaccg ctacctgccc tacatcgtgc
      421 tggtggtgga cgagttcgcc gacctgatca tgactgcggg ccgcgaggtg gagaccccga
      481 tcgctcgtct ggcccaattg gcccgcgcca tcggcatcca cctgatcatc gccacacagc
      541 gccccagtgt gaacatcatc acggggacga tcaaagcgaa cttcccggcg cgcatcgcgt
      601 tccgcgtaag cagcaaggtg gacagccgca cgatcctgga tgccggagga gccgaccagt
      661 tgatcggtcg tggcgacatg ctgctgagca ccggcaacga cctcatccgg atccagtgcg
      721 ccttcgtgga cacgcccgag gtggaaaaga tcaccgattt cattggcggc caacgcggct
      781 accccgatgc catgctcctg ccagagccac ccatggccga gggcgaaggc ggcgaggaca
      841 tggacgatgg cgagcgcgac agcatgttcg aggaagccgc acgtctggtg gtgcagacac
      901 agcagggcag cacctcgttg atccaacgca agctgaaact cggttataac cgcgccggtc
      961 gcattgtgga ccagttggag tcggccggca ttctcggccc tttcgaaggc agcaaggcac
     1021 gccgtgtgat gatcccgaac gaagctgcgc ttgcggctca cctcaacacc ggcgtgacca
     1081 ccggacccgg gatgggcggt ttctgatcat tgggcgacgc ggttctgcgc tctggacagc
     1141 ctcctcattc gggaatattg gccacatcag cgatggccta tcccctaccc gctcatgatc
     1201 cgagttattg atccggccgg gaacgaactt cgaagcacac ggtctgttct ggtggtgccc
     1261 ggaacatgtt cgagatggaa cagcccgttt catcccatga gccagccttg accatgaagc
     1321 tcattcgaag cgcctcccgg aacacagcac ggccttgacc cggctatacc ggtcgcactt
     1381 ctgttccgga tgatctgtgc cacaagccga tcctcacttc ttcttccgtt ccatcatctt
     1441 gatccgcact ccctgtttgg caagtcttcc caccgccaac gcggccaggg tgtgtgtccg
     1501 gtcgatggtc aggcagatct ggtagttggc ggccgcactg tccggttgtt gggtgcgttc
     1561 catggctatc ccccggctgt accaggcgtc ggcatagttg gtgttgaggt cgatcgcctt
     1621 ggagaaatcg gccttggcac cctgtgggtc attgaggtgt tccagtttga tgaaaccgat
     1681 attgtaccaa ggcaacgggt tcatcagatc cagttccttg atccggtcgt agcaggcgat
     1741 ggccaggcta tcgtggccgt tgttctgcgc ccatagccct ttgccgtacc aggcttccac
     1801 cgaccggggc cgcagctcga tcgcggtcgt ataatactgc tcggccagcc ggtcgcgacg
     1861 tgatgcgctc agttgaccga gcaacatgta cgatgggtag tcctgcggat cctgttccac
     1921 agcggtgcgg aagctgctga tggcccgcga ggtgtccttg gtctccatgt ggatgtagcc
     1981 cttgaggaaa tagccccgag ccgcgttcgg atcgatgcgc agggcttcgt tcacgagttc
     2041 catggccttg ctgtagtcgc ggagcaccat gtacacctca cccagtcgca gtttggcttc
     2101 ggtgctggtc ggtgccagat ccaccgcacg ttggaacgca tcccgggcct cgcccatctt
     2161 gatcctgcgg tagtgcagat caccgagatg aatgcgg
//
LOCUS       DAOQOC010000481         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31884_length_2187_cov_2.675422, whole genome shotgun sequence.
ACCESSION   DAOQOC010000481 DAOQOC010000000
VERSION     DAOQOC010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31884_length_2187_cov_2.675422"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="PL106_08355"
     CDS             <1..1459
                     /locus_tag="PL106_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNA33119.1"
                     /translation="DIATPLLPAAVMERAARACTDWLLRHVPPRGRAFLVLAGCGNNG
                     GDGLAVARMLLAAGARVRVVHCRHREYLSHDARINLERLRTLPVSILDAEDGSILDDP
                     PEDEVVIDALLGIGLDRPVNGWLKQVIMQVRAWPNQVISIDLPSGLFADDNTTNDPQA
                     IVRADHVLTFEVPKPALLLPENGRSIGLWHLLPIGLDRTYMDDLGSKDFLVEASDALE
                     LMPERPRAAHKGTFGHALLVAGNPGKAGAAVLAARACARSGAGLITVATCPDLQQVIH
                     ASLPEAMVVSDPFEKSVDMARFTAIGIGPGMGTSDDAARLLKRLLQDATAPLVLDADA
                     LNILAENRTWLAFLPKHTILTPHPKEFDRLTEKSASSAERSAKARAFAVRFGVTLVLK
                     GAYTATCAPDGLVYFNPTGNAGMAKGGSGDALTGVIAALLAQGLTPLSAAVLGVYAHG
                     LAGDLAAEDLGRDGMLPSDLIDRLPLAWRKIRASG"
     gene            complement(1456..1812)
                     /locus_tag="PL106_08360"
     CDS             complement(1456..1812)
                     /locus_tag="PL106_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNA33120.1"
                     /translation="MKAALPGPYTFVVPANSEIPRIFKNNKRTVGIRVPDHPVPIALV
                     KALGHALVCASVHGMDVLLEHAPDPEAIHAQYGHAVEAVIDSGPGGLEGSTVIDLSGP
                     EPVVLREGKGAIEELL"
     gene            1811..1942
                     /locus_tag="PL106_08365"
     CDS             1811..1942
                     /locus_tag="PL106_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33121.1"
                     /translation="MMRKDGVLIARAPFIEALALPAHEVEQSLRVMRAAAARDAVPR"
     gene            complement(1952..>2187)
                     /locus_tag="PL106_08370"
     CDS             complement(1952..>2187)
                     /locus_tag="PL106_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33122.1"
                     /translation="VTEVYGLGNLRAKGVAISGWRTVTFDVLLTDAPCIPRWYADTGK
                     GVRRMLSPNTPRYRLRLYAAAWTAAVAAVPAGK"
BASE COUNT          359 a          714 c          685 g          429 t
ORIGIN      
        1 tgacattgca acccccctgc tacccgccgc cgtgatggag cgcgcagcac gggcctgtac
       61 ggattggttg ctgaggcatg ttcccccccg gggtcgcgct tttcttgtgc ttgcgggctg
      121 tggtaacaac ggtggggatg gtttggccgt ggcccgcatg ctgcttgccg caggcgcacg
      181 tgtgcgcgtt gtgcattgcc gccaccgcga gtacctcagc catgatgccc gcatcaacct
      241 tgagcggctt cgcaccttgc ccgtatcgat ccttgatgcc gaggatggat cgatcctgga
      301 tgatccacct gaagatgagg tggtgatcga tgcgctgctc ggcatcggcc tggaccgccc
      361 ggtaaatggc tggctgaagc aggtgatcat gcaagtgcgg gcctggccca accaggtgat
      421 ctccatcgac ctgccctccg ggttgttcgc tgacgacaat accacgaatg atccccaggc
      481 cattgtacgg gccgatcatg tcctcacctt cgaggtgccc aagcccgctc tgttgcttcc
      541 ggagaatggg cgatccatag gactatggca cctgttgccg atcggcttgg atcgaacata
      601 catggacgat ctgggctcca aggacttcct ggtggaggct tcggacgctt tggaactgat
      661 gcctgaacgt ccgcgtgccg cacacaaggg gaccttcgga catgctttgc tggtggccgg
      721 aaacccgggc aaggcgggtg cggccgtgct cgctgctcgg gcctgcgcac gctcgggtgc
      781 cggtctgatc acggtggcca cgtgccctga tcttcagcag gtgatccatg cgagcttgcc
      841 ggaggcgatg gtggtatccg atccattcga gaagtccgtt gacatggccc gcttcaccgc
      901 tatcggcatc ggtccgggca tggggacatc cgatgatgcg gcccgcctgc tgaagcgtct
      961 gctgcaggat gccaccgctc ccctcgttct ggatgccgat gcgctcaaca tcctggccga
     1021 gaaccgtaca tggctggcct tcctgcccaa gcacacgatc ctgaccccgc atcccaagga
     1081 gttcgatcgg ttgaccgaga agagcgcctc cagcgctgaa cgatcggcga aagcacgcgc
     1141 attcgcggta cgcttcggcg ttacactggt gctcaagggt gcatacacgg ccacctgcgc
     1201 gccggacggc ctggtctact tcaaccccac gggcaatgcg ggcatggcca agggcggaag
     1261 cggcgatgcg ctcaccggtg tcatcgcggc actgctggcc caagggctga caccactttc
     1321 cgccgccgta ctcggggtgt atgcgcatgg tcttgccggt gatctggccg cggaagacct
     1381 gggcagggac ggtatgctgc ccagcgacct gatcgaccgt ttgccattgg cgtggagaaa
     1441 gatccgcgct tccggttaga gcagttcctc gatcgcgcct ttgccttcgc gcagcaccac
     1501 aggctccgga ccactgaggt cgatcaccgt actgccttcc agcccgcccg gcccgctgtc
     1561 gatcacggct tccaccgcat ggccatactg cgcgtgtatg gcctcgggat ccggtgcgtg
     1621 ctccaggagc acgtccatgc catgaacact ggcgcatacc aatgcatgtc cgagcgcttt
     1681 caccagggcg atgggcaccg gatggtccgg tacccggatc cccacggtcc ttttgttgtt
     1741 cttgaagatc cgcgggatct cgctgttggc gggtaccacg aacgtgtatg gacccggcag
     1801 cgccgccttc atgatgcgga aggacggcgt gctgatcgcc cgggctccct tcatcgaagc
     1861 cctcgccctc ccggcccacg aggtcgaaca gtccctcagg gtcatgcggg ccgccgcggc
     1921 ccgcgacgcc gttccacgct gaccgggcgc gtcacttccc ggccggcacg gcggccacgg
     1981 ccgcggtcca agccgcggcg tacagccgca ggcgatatct gggtgtgttg gggctcaaca
     2041 tgcggcgcac cccctttcct gtgtcagcgt accaccgtgg gatgcagggc gcatcggtca
     2101 aaaggacgtc aaacgtcact gtcctccaac ctgatatggc cacgcctttg gcacgcaggt
     2161 tgccaaggcc gtagacttcc gttactg
//
LOCUS       DAOQOC010000482         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31910_length_2186_cov_3.126232, whole genome shotgun sequence.
ACCESSION   DAOQOC010000482 DAOQOC010000000
VERSION     DAOQOC010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31910_length_2186_cov_3.126232"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PL106_08375"
     CDS             complement(<1..902)
                     /locus_tag="PL106_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNA33123.1"
                     /translation="MADGQRIVVVGGGAAGFMAAIAARSFRPDASVVLYERSEKWLAK
                     VRISGGGRCNVTHDRSDPKQLARNYPRGEAFLRKVFKAWGQPETVKWFAAQGLGLKAE
                     KDGRMFPLSDDSASVIAVLWNAAERAGVELRARNGISSLRFREDRWHLTSAAGPLEAD
                     RVVLATGGSPKIEGLAWIAALGHAIVPPVPSLFTFDLVDDPVRQLMGVVAPEVRLRLE
                     GSGLESTGPLLITHWGFSGPAVLRLSAFGARILHDLDYRFTVQVNWLGVDGGPHRFRE
                     VLRTVSTTHPHRHLANIEGIELPKR"
     gene            1116..1607
                     /locus_tag="PL106_08380"
     CDS             1116..1607
                     /locus_tag="PL106_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNA33124.1"
                     /translation="MAPADIQQNVHRLFSEYLEHGGHRKTPERFAILREIYAVDGHFD
                     IELLYGRMQQTKCQVSRATLYNTMDLLLDARLVRKHQFGTTQAQFEKAQIRGQHDHVI
                     CDGCGKVQEFCDPRIQNVRNTVSEVLGFRVSHHALHIHGICADCQKKSNERTKAARAA
                     VQP"
     gene            1604..1975
                     /locus_tag="PL106_08385"
     CDS             1604..1975
                     /locus_tag="PL106_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNA33125.1"
                     /translation="MSTITERPPFGLSVSQHGRNKIFHMTGRLMDQKEADHMIEVLEQ
                     ELGSGTGANVILDMSQLQYMNSTGLNILISVLSRTRKGGGETLISGMSTGVKQLFTVT
                     KLDTVFTIKDTVEEALGSLPK"
     gene            1980..>2186
                     /locus_tag="PL106_08390"
     CDS             1980..>2186
                     /locus_tag="PL106_08390"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNA33126.1"
                     /translation="MANRVDVLLGAQWGDEGKGKVVDVIAPGYQVIARFQGGPNAGHT
                     LIFDGKKHVLHTIPSGVFRPGTWNL"
BASE COUNT          484 a          708 c          643 g          351 t
ORIGIN      
        1 cgtttcggta gttcaatacc ttcgatgttg gccaggtgcc tgtgcggatg ggtggtgctc
       61 acggtcctca gtacctcacg gaagcgatga ggaccgccat caacgcccag ccagttcacc
      121 tgaacggtga agcgatagtc cagatcatgc aggatgcgcg caccgaacgc actcaagcgc
      181 agcacggccg ggccgctgaa gccccaatgg gtgatcagga gcggacccgt gctttccaga
      241 ccggaacctt cgagccgcag ccgcacttcg ggggccacca cacccatcag ctgacgaaca
      301 ggatcatcca ccagatcgaa ggtgaagagg gagggaacag gtggcacgat cgcatgcccg
      361 agtgccgcga tccaggccag tccttcgatc ttgggcgaac cgcccgtggc cagcaccacc
      421 cggtcagcct ctagcgggcc cgccgcactt gtgaggtgcc accgatcctc acggaaccgc
      481 aatgacgaga taccgttccg cgcgcggagc tccacccccg cccgttcggc agcgttccaa
      541 agcacggcga tcaccgaagc cgaatcatcc gagaggggga acatgcgtcc gtccttctcc
      601 gccttgagtc caaggccttg cgctgcgaac catttcaccg tttcgggctg gccccaggct
      661 ttgaacacct tccgaagaaa ggcctcaccg cgtggatagt tgcgcgccag ttgcttcggg
      721 tcgctgcggt cgtgcgtcac gttgcaacgc cccccaccgc tgatgcgcac cttggccagc
      781 catttctcgg atcgctcgta cagcaccacc gaggcgtccg gccggaacga tcgggccgcg
      841 atggccgcca tgaaaccggc ggcacccccg cccaccacca cgatccgttg accatccgcc
      901 acgcacgcac aaagaaaaca gcaccaccgc tctccggggc agggtacatc gagttgtgga
      961 gtcacatgga cacaaagtca aggagtggtc ccgttattgg atccacgctg cacgaacgga
     1021 tcagcccccc cgcggccgat cacatcgggt gcgttcaaaa gcccgcacac gcgccttcgg
     1081 ccaaaggcac cggaccagct accttcgcgc acgatatggc gccggcggac atccagcaga
     1141 acgtgcatcg cctcttcagc gaatacctgg agcacggtgg gcaccgcaaa acgcccgaac
     1201 gtttcgccat cctgcgcgag atctacgcgg tggatggcca tttcgacatc gaactgttgt
     1261 atggccggat gcagcagacc aaatgccagg tgagcagggc cacgttgtac aacaccatgg
     1321 acctgctgct tgatgcacga ttggtgcgca agcatcagtt cggcaccacg caggcccaat
     1381 tcgaaaaagc gcagatccgg ggccagcacg accacgtgat ctgcgacggt tgtggcaagg
     1441 tgcaggagtt ctgcgacccc cggatccaga acgtccggaa caccgtttcg gaggtgctgg
     1501 gcttccgggt gagccaccac gcgctgcaca tccacggcat ctgcgccgat tgccagaaaa
     1561 aaagcaacga acgaaccaag gccgcacgag cggcggtaca accatgagca cgatcaccga
     1621 acgtcctccc ttcggcctct ccgtgagcca gcatggccgc aacaagatct tccacatgac
     1681 cggccgcctg atggaccaga aagaggctga ccacatgatc gaggtgcttg agcaggaact
     1741 cggcagtggt accggtgcga acgtcatact ggacatgagc cagttgcagt acatgaacag
     1801 cacagggctc aacatcctga tcagcgtgct cagccgcacg cgcaaaggcg gaggcgaaac
     1861 gctgatcagc ggcatgagta cgggcgtgaa acaactcttc accgttacca aactcgatac
     1921 ggtgttcacg atcaaggata ccgtggaaga ggcattggga tccctgccca agtagcatta
     1981 tggcgaaccg cgtggacgtg ctgctcggtg cccaatgggg cgacgagggc aaaggcaagg
     2041 ttgttgacgt gatcgcacct ggataccagg tgatcgcgcg tttccaaggg ggacccaacg
     2101 cgggtcacac cctcatcttc gatgggaaga agcacgtgct gcacaccatc ccgagcgggg
     2161 tgttccgtcc gggcacctgg aacctg
//
LOCUS       DAOQOC010000483         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31958_length_2184_cov_3.146078, whole genome shotgun sequence.
ACCESSION   DAOQOC010000483 DAOQOC010000000
VERSION     DAOQOC010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31958_length_2184_cov_3.146078"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PL106_08395"
     CDS             complement(<1..225)
                     /locus_tag="PL106_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33127.1"
                     /translation="MPRNRRTTVLLAILAVVSVVALVQYWPTTPPPVAVTGAPARPAG
                     RPSTATSITAPDVHLQALSAERPRPQDEPER"
     gene            complement(215..1078)
                     /locus_tag="PL106_08400"
     CDS             complement(215..1078)
                     /locus_tag="PL106_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNA33128.1"
                     /translation="MTFSSHLIPLRNTIALCLTLVATWASAQCDTVFIDHTGWTVTYV
                     DSEELTGEGPDNGHAIHAIDGDSTTFWHTEWQAADPPFPHEIHLDLGAVHAVNGLSLL
                     SRHNSANGKIIGYALYLSMDGVAWGTPQAAGNLTYPDVNAPGQRATVYFGAVDARFIK
                     LVATSNNNGTQHAMLAELQLTEYVGSGCGATGQNNQFVSVDPVPLQETNGPDITLNGT
                     ASSGLPLTWSVLSGPATVVGNTLSLTGAAGTVVVKAEQPGDATWYAASATTSFNVIDL
                     STYYRVRPDAS"
     gene            1267..2121
                     /locus_tag="PL106_08405"
     CDS             1267..2121
                     /locus_tag="PL106_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNA33129.1"
                     /translation="MDRTIPLPKILLLPLRGLGQIMLQENAATGLLFLIGIAWASLVM
                     ALAAWLAVCCGTATAILLKYDRAEIEQGLYGFSAALVGVALVLFRGIGPLVWLAIVAG
                     SVGAAIVQRFFIARKIPVFTLPFVLVTWVLLCVLPVPTGLVDGAPMAAGPVHYSTAVV
                     ARGFGQVIFQDNLFSGILFMVAVALHSRIAVVFGVFGALLGALLAMWIGAPADNVMLG
                     LYGYNAVLCAIAFAGTKARNYLLFLLSVPLAVLIGHAMMELPFPQLTFPFVVASCSGL
                     LLEKVTRD"
BASE COUNT          314 a          626 c          767 g          477 t
ORIGIN      
        1 ccgctcgggt tcgtcctgtg gacgcgggcg ttccgcgctg agcgcctgga gatgcacgtc
       61 tggcgccgtg atgctggtcg ccgtggacgg ccgccccgcc gggcgggccg gcgcccccgt
      121 cacggcaacg ggcggcggcg tcgtcggcca gtactgaacc agcgcgacga cgctcaccac
      181 cgcgaggatc gcgagcagga cggtcgtgcg tcggttacga ggcatccggt cgcacgcggt
      241 agtaggtgga gaggtcgatg acgttgaaac tggtggtggc cgatgccgcg taccaggtgg
      301 catcaccggg ctgttcggcc ttcacgacca ccgtgcccgc tgcgccggtg agcgacagcg
      361 tgttgcccac caccgtggcc gggccgctga gcacgctcca ggtgagcgga agcccgctgc
      421 tggctgtgcc attgagggtg atgtccggcc cgttggtctc ctgcaaggga acgggatcga
      481 cgctcacgaa ctggttgttc tgcccggtgg cgccgcatcc ggaacccacg tattcggtga
      541 gttgcagttc ggcaagcatg gcgtgctgcg taccgttgtt gttggaggtg gccacgagct
      601 tgatgaagcg tgcatccacc gcaccgaagt acacggtggc gcgttgcccc ggcgcgttca
      661 cgtcgggata cgtgaggtta ccggcggcct gtggcgttcc ccatgccacg ccgtccatgc
      721 tgaggtagag cgcgtagccg atgatcttgc cgtttgcgct gttgtggcgc gagagcagcg
      781 aaaggccgtt caccgcatgc acggcaccga ggtccaggtg gatctcgtgc gggaagggcg
      841 ggtcggcggc ctgccactcg gtgtgccaga acgtggtgct gtccccatcg atggcgtgga
      901 tggcgtggcc gttgtccggt ccttcgccgg tgagttcctc gctgtccacg taggtcacgg
      961 tccagccggt atggtcgatg aacaccgtgt cgcattgggc gctggcccag gtggcgacaa
     1021 gggtgaggca cagggcgatg gtgttccgca aggggatgag gtgtgaagaa aaggtcatcg
     1081 gcaacgatgt gggctggaat ggtttcgcga aggtaccttg gtggcgtgtg gtcgatcgcc
     1141 ggatccaaga ctttcagatg atcttgcagt ggatcagggt tccaagcgga cctgttttca
     1201 tgtttgttcg ttggacggga tcctacgggt aggtgaacat gcaggaactt cgcacgatcg
     1261 atcctcatgg accgcacgat accgttgccc aagatccttt tgctgccact gcgtggcctg
     1321 ggccagatca tgctgcagga gaatgccgct acggggctgc tgttcctgat cgggatcgcg
     1381 tgggcttcgc tggtgatggc ccttgcggcg tggctcgctg tgtgttgcgg taccgccaca
     1441 gcgatcctct tgaagtatga tcgtgcggag atcgaacaag ggctctacgg cttcagtgcg
     1501 gcgctggtgg gtgtggcact cgtgctgttc cggggcatag gaccgctcgt gtggctcgcg
     1561 atcgtcgcgg gaagtgtcgg ggctgccatc gttcaacggt tcttcatcgc acgaaagatc
     1621 ccggtgttca cgttgccttt tgtgctggtg acctgggtgt tgttgtgtgt gttgcccgtg
     1681 ccgaccggac ttgtcgacgg tgctccaatg gcagcaggcc ctgtccatta cagcacagcg
     1741 gtcgtggccc gtggcttcgg ccaggtgatc ttccaggaca acctgttctc cgggatcctg
     1801 ttcatggtcg cggtggccct gcattcgcgc atcgccgtgg tcttcggcgt gttcggcgcc
     1861 ttgctcggtg cgttgttggc catgtggatc ggtgcgcctg cggacaacgt gatgctgggt
     1921 ctttacggct acaacgccgt gctctgcgcc atcgccttcg cgggaacgaa agcgcggaac
     1981 tacctgctgt tcctgttgtc cgtgccgctc gccgtgttga tcggccacgc gatgatggag
     2041 ctgccctttc cgcagctcac gttcccattc gtggtggcaa gctgctccgg cctgctattg
     2101 gagaaggtca cccgcgattg aactgtgctt ggacttggcg gtggacgcgg tccacacagc
     2161 gttgatcctg cgtgtgctca ttgg
//
LOCUS       DAOQOC010000484         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_31989_length_2183_cov_2.477914, whole genome shotgun sequence.
ACCESSION   DAOQOC010000484 DAOQOC010000000
VERSION     DAOQOC010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31989_length_2183_cov_2.477914"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PL106_08410"
     CDS             <1..537
                     /locus_tag="PL106_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33130.1"
                     /translation="AGAQFTLSKAVALAVHDMVTGALGGSAVEVRIKWPNDVLCGSRK
                     IAGILIASELMGSQVRNSIVGIGINLNGTGFPEGLNATGLRLETGRDHDRMVVLGGLC
                     ERLEHWLNRWQAGSDEIAVAYADRLWARGRWVAFELDGVPFTARLMDVDVAGRLLVED
                     EAGEVRALGHDRLRLVRA"
     gene            639..797
                     /gene="rpmH"
                     /locus_tag="PL106_08415"
     CDS             639..797
                     /gene="rpmH"
                     /locus_tag="PL106_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNA33131.1"
                     /translation="MKRTYQPSKRKRVNKHGFRKRMASATGRAVLASRRKQGRKKLTV
                     SDEGIGKK"
     gene            complement(1143..1964)
                     /locus_tag="PL106_08420"
     CDS             complement(1143..1964)
                     /locus_tag="PL106_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNA33132.1"
                     /translation="MAKTETKSPVKEEKSPKTANPATKAEITIAEKLVELYRLQSIDS
                     QTDRIRTQRGELPLEVQDLEDEVTGLETRLKKLEDELAEAEQAVSDKTNFIKDAKAAI
                     KKYEGQQAKVRNNREYDSLSKEVEFQNLEIQLAEKRIKEGKFTIDSKKSVVDESTKLY
                     KERKKDLDLKKKELDDIISETEKEEKDLQKKSDAAAKHIEERLLSAYRRIRGNARNGL
                     AVVPFERGSCGGCFNAIPPQRQLDIRSHKKIIVCEHCGRILVDEYVVERVKGEGN"
     gene            complement(2012..>2183)
                     /locus_tag="PL106_08425"
     CDS             complement(2012..>2183)
                     /locus_tag="PL106_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNA33133.1"
                     /translation="KYHEFFGAEGQLVVADIGHFESEQFTMHLLQRQLRDKLPTFAAH
                     LTETVSNPVHVR"
BASE COUNT          381 a          653 c          679 g          470 t
ORIGIN      
        1 gccggggcac aattcaccct gtccaaagcg gtggccctcg ccgtacatga catggtgacc
       61 ggggcacttg gtggatcggc cgttgaggtg cgtatcaaat ggccgaacga tgtgctgtgc
      121 ggctcgcgca agatcgcggg catcctgatc gcgagtgagt tgatgggcag ccaggtccgc
      181 aacagcatcg tggggatcgg catcaacctg aacggcaccg gctttccgga aggcttgaat
      241 gccacggggc tgcgcttgga aaccgggcgc gaccatgacc ggatggtagt gctgggaggg
      301 ttgtgcgaac ggttggaaca ctggttgaac aggtggcagg cggggtcgga tgagatcgct
      361 gtggcgtatg ccgaccggtt gtgggcacgc gggcgctggg tggcctttga attggacggc
      421 gttcccttca ccgcccggct gatggacgtg gatgtcgccg ggcgattgct ggtggaggac
      481 gaggccgggg aggtgcgcgc gctgggtcac gaccggttga ggctggtacg ggcatagcaa
      541 ggccgtttgc atggatcgac cgatcggcta catttgcggc cctttcggga aaaactgacc
      601 tcaacaccca cgttccagcg ttctggaaca gcagcagcat gaaacgcacc taccagccca
      661 gcaaacgcaa gcgcgtcaac aagcacggat tccgtaagcg catggcgtcg gcaaccggcc
      721 gtgccgtgct ggcgagccgc cgcaaacaag gccgcaagaa gctcacggtg agcgacgagg
      781 gcatcgggaa gaagtaaccc gttctcaaca catccgatca gagggcctgc cattcagcag
      841 gccctttgcc attcctggcc gggaccaact gtggccgcaa aacggggatc aggaagcata
      901 gaagcatgtg cgcggccgct tgttcagcat actgttccca cggcaccaag cggtgccaca
      961 acagacctca ttcaagatgt ccaagaactg tcacgctgag cccgccgacg gttcggcaag
     1021 ctcaccgctt cggcgggccc agcgtgacag ttcagcagaa tgcgcggatg cggaccgtga
     1081 aagaggccca acgaaaaagc ccccggataa ccgggggctt tcgctatctt ttgtgggcag
     1141 gatcagttgc cctccccttt cacacgctcc accacgtact catccacgag gatgcggccg
     1201 caatgctcgc acacgatgat ctttttatgg ctgcggatgt ccagctggcg ctggggcggg
     1261 atggcgttga agcagccgcc acagctaccg cgctcgaagg gcaccacggc caggccgttg
     1321 cgggcgttgc cgcggatccg cctgtacgcg ctcagcaggc gctcctcaat gtgcttggcc
     1381 gcagcgtcac tcttcttctg caggtccttc tcctccttct cggtctcgct gatgatgtcg
     1441 tccagctcct tcttcttgag gtcgagatcc ttcttgcgct ccttgtacag cttggtgctc
     1501 tcgtccacca cgctcttctt gctgtcgatg gtgaacttgc cctccttgat gcgcttctcg
     1561 gcgagttgga tctccaggtt ctggaactcc acctccttgc tcaggctatc gtactcgcgg
     1621 ttgttgcgca ccttggcctg ctggccttcg tacttcttga tggccgcctt ggcatccttg
     1681 atgaagttgg tcttgtccga cacggcctgt tcggcttccg ccagttcgtc ctcgagtttc
     1741 ttcaggcggg tctccaggcc ggtcacctca tcctccaggt cctgcacctc caacggcagt
     1801 tcaccacgct gggtgcggat gcggtccgtt tgcgaatcga tgctctgcaa gcggtagagt
     1861 tcaacgagtt tctccgcgat cgtgatctcc gccttggtgg cgggattcgc cgttttcggg
     1921 gatttctcct ccttcaccgg gctcttggtc tcggtcttgg ccatgctaac gcgctttggg
     1981 gtgttgggac cgtccttccg cgctgcacga ttcagcgcac atgcacgggg ttggaaacgg
     2041 tttccgtcaa atgggctgcg aatgtaggca gtttgtcgcg tagttggcgc tggagcaggt
     2101 gcatggtgaa ctgttcgctt tcaaagtgcc caatgtcggc cacaaccaac tggccctcag
     2161 cgccgaagaa ctcgtggtac ttc
//
LOCUS       DAOQOC010000485         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32020_length_2182_cov_2.904090, whole genome shotgun sequence.
ACCESSION   DAOQOC010000485 DAOQOC010000000
VERSION     DAOQOC010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32020_length_2182_cov_2.904090"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PL106_08430"
     CDS             complement(<1..713)
                     /locus_tag="PL106_08430"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNA33134.1"
                     /translation="METLKTGKIQDLLGADTDALLGHTCKTIDKQALNLPGPDFVDRC
                     FAASDRSPQVLRSLQQLYGSGRLAHSGYMSILPVDQGIEHSGGASFAPNPIYFDGENI
                     VKLAIEGGCNAVASTYGVLGSVARKYAHRIPFMLKFNHNELMTLPNKFDQVLFGTVEE
                     AWNLGAVAVGATIYFGSDEASRQITEIAEAFQFAHELGMATVLWCYLRNPGFKKDGTD
                     YHVSADLTGQANHLGVTIQA"
     gene            complement(985..>2182)
                     /locus_tag="PL106_08435"
     CDS             complement(985..>2182)
                     /locus_tag="PL106_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNA33135.1"
                     /translation="TSLQASITVGMEAQQSLTPDDDQTGDASTSLARNALLNTLEIGP
                     ELKVFVPIRGGSAMAGGSRGVVNALFNYQRRPDYTRTLAKGAIGYEWNFSFISACGVY
                     FPEVNVVRIPLRSHAFEQFLNDATDPVLTNSYTDHIIVNLPRVTWTYNTANAPGPARR
                     NNQFIRLNAELAGTILRLVQQASVPAQGWDTITSAGYYTLGDARYAEYFKFDADLRHY
                     HTIHDRSSIVFRFAPGFAKPFGNQHVMPFETSFYSGGANGIRAWRARSLGPGGYWSDV
                     NRFDRIGELRLEGNVEYRFKLIGYFEGALFADAGNIWYLQDNPAKPGSQFRWNQFYKQ
                     IALGTGMGLRLNLEFLLLRLDLSLQTHDPALPDGQRWIYQRRSAPYDAPFSRRLNFNL
                     GIGYPF"
BASE COUNT          392 a          619 c          724 g          447 t
ORIGIN      
        1 gcctggatcg tgacgccgag gtggttggcc tggccggtga ggtcggcgct cacgtggtag
       61 tcggtaccgt ccttcttgaa ccccgggttg cgcaggtagc accagagtac ggtggccatg
      121 cccaattcgt gcgcgaactg gaaggcttcg gcgatctcgg tgatctgacg gctggcctcg
      181 tcgctgccga aatagatggt ggcacccacg gccacggccc ccaggttcca cgcttcctcc
      241 accgtgccga agagcacctg gtcgaacttg ttcggcaggg tcatcaactc gttgtggttg
      301 aacttcagca tgaacgggat gcggtgcgcg tatttccggg ccacactgcc gagcacgccg
      361 taggtgctgg ccaccgcatt gcatccgccc tcgatggcca gtttcacgat gttctcgccg
      421 tcgaagtaga tgggattggg ggcgaagctg gcaccaccac tgtgctcgat gccctggtcc
      481 acgggcagga tgctcatgta tcccgagtgg gccaaacggc cgctgccgta caactgctgc
      541 aagctccgca aaacctgcgg gctgcggtcg ctggcggcga agcagcggtc cacgaagtcg
      601 ggacccggca ggttgagcgc ttgcttatcg atcgtcttgc acgtatggcc caggagcgcg
      661 tccgtgtcgg cacccagcag gtcctggatc ttccccgtct tcaatgtttc catccgttgg
      721 ttcggttgcg gtggcgaact tacgccaggg cggtccgggc gggaaatagc tccggccgaa
      781 acgtgaagtg gccctcgtgg aaaagtcccg ccccggccca gaaccggttc gcgatcaccg
      841 catttggctg tgcgttggtc tttgacgacc atactttgca atggaagcgg gcacgtagct
      901 ccggctatgc tcctgttcct gcatcgcgcc tggtcaccaa ttgcatttgc tcgttgaagt
      961 caaggggcaa ttgcgatcag gttctcagaa cggatagccg atgccgaggt tgaagttgag
     1021 gcgcctgctg aaaggcgcat catagggggc gcttcgtcgc tgatagatcc agcgttggcc
     1081 atcgggcaag gccggatcgt gggtttggag ggacaggtcc agacgaagga gaagaaattc
     1141 caagttgagt cgcaagccca tgccagtgcc cagcgcgatc tgcttgtaga actggttcca
     1201 tctgaattgg ctgccgggtt tggcgggatt gtcctgcaga taccagatgt tgccggcgtc
     1261 ggcgaacagg gcgccttcga agtagccgat gagtttgaag cggtactcca cgttcccctc
     1321 gagcctcaat tcgccgatcc ggtcaaagcg gttcacatcg gaccagtagc cgcccgggcc
     1381 gagcgatcgt gcgcgccagg cacggatacc gttggcacca ccgctgtaaa aactcgtttc
     1441 gaaaggcatc acgtgctggt tgccgaaggg cttggcgaag ccgggagcga accggaagac
     1501 gatgctgctc cggtcgtgta tggtgtggta gtgccgcagg tccgcgtcga acttgaaata
     1561 ctcggcgtag cgcgcatctc ccaaggtgta gtaaccggcc gaggtgatgg tgtcccagcc
     1621 ctgtgccggt acggaagcct gctggaccag gcggaggatc gtcccggcca gctccgcgtt
     1681 caagcggatg aactggttgt tccggcgggc agggccgggt gcattcgctg tattgtaggt
     1741 ccaggtgacc cgcggcaggt tgacgatgat gtggtcggta tagctgttgg tgagcaccgg
     1801 gtcggtcgcg tcgttcagga actgctcaaa ggcgtgcgat cgcaagggga tccgcaccac
     1861 gttcacttcg gggaaataga ccccgcaggc cgagatgaag gagaagttcc attcgtaacc
     1921 gatggcgcct ttggccaggg tgcgggtata gtcgggtctg cgttggtagt tgaacaacgc
     1981 gttgaccaca ccgcggcttc ccccggccat ggccgagcca ccgcggatgg gaacgaatac
     2041 cttgagttcg ggacctattt ccagggtgtt cagaagggcg ttccgggcca gcgaggtact
     2101 ggcgtccccg gtctggtcat cgtccggggt gaggctctgt tgcgcttcca tgcccacggt
     2161 gatcgaagct tgcaacgagg ta
//
LOCUS       DAOQOC010000486         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32130_length_2178_cov_3.316533, whole genome shotgun sequence.
ACCESSION   DAOQOC010000486 DAOQOC010000000
VERSION     DAOQOC010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32130_length_2178_cov_3.316533"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PL106_08440"
     CDS             <1..657
                     /locus_tag="PL106_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33136.1"
                     /translation="NDNPLPGNGITGNTTDRPVTRWYGKVQSNGFGTPTTGNYLNYGG
                     GLVLDTNAAPGAISDAAYRGPGTIRAMRAVYDGTTQLSDHVFDSYFDGEVAPEDMHAA
                     DDYTYVALPRLKEHLEKDRHLPNMPSRSEWEEHGTRPIGALQTGLWESVETQALYIAQ
                     LEQDLTTLEALAFGGVSSAEELDRLVAEVRSSRRLSEKQKLHLTNALRARYTKSSGTK
                     "
     gene            678..>2178
                     /locus_tag="PL106_08445"
     CDS             678..>2178
                     /locus_tag="PL106_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33137.1"
                     /translation="MKRFLLRSLPAVAVIGLTANVNAQNRGVAVNATGAAPNDMAILD
                     VSSTTKGMLIPRMTLAQRNAIAAPIPQGLTVYVDDAVNTNDGFWIREYNTWVRLDIGA
                     DGWDINGNNVGAPATDFFGTRDNVDLVFRTNNAQVMRVSGTTVNNGRYTHIGTNITAP
                     NEQLDIGSGSPSTGALRIYNPAPGTWAATNPMVKYAATNTQGTIHFRPIGALGATGDS
                     LEATFNPPGYPTQQSRDVVMWNGHWGNITGTAPVLNTATAHSGGWKRLENEYIEIFNK
                     AFTQQATPVCGNTPVELTQGSTTYTSGLLAADALLINPYKFDMPGRRYRAQYLFLPDE
                     LNVERNQLVLGNLNATGGLCAGSPMNSIGFYVNMNVTTQTRAANVTITVKHAPLGTTM
                     LTGFDDSVDPAQGCFNNAALNPFPTAGGPGWKNYTLTTPFTWDGERPVIVEVAVHQAA
                     AGGGGTLPGGLPIRYSTTGTTTTYSAYTTFPAGIGAPGSPCNPNVPTFST"
BASE COUNT          458 a          728 c          654 g          338 t
ORIGIN      
        1 aacgacaatc cgctgccagg caatggcatc accggcaaca ccacggaccg cccggtaaca
       61 cgttggtacg gcaaggtaca gagcaatggc ttcggcacgc ccaccacggg caactacctc
      121 aattacggcg gggggctggt gctcgacacc aatgcggcac cgggagccat ttccgacgct
      181 gcctaccgcg ggccgggtac catccgggcc atgcgtgcgg tgtatgatgg caccacccaa
      241 ttgagcgacc acgtgttcga cagctatttc gacggcgagg tggctccgga ggatatgcac
      301 gcggcggacg actataccta cgttgcgcta ccccggctga aggaacacct ggaaaaggac
      361 cggcacctgc ccaacatgcc ctcgcgcagc gaatgggagg agcacggcac ccgaccgatc
      421 ggggccttgc agacgggcct gtgggaatcg gtggagaccc aggcgctgta catcgcccaa
      481 ttggaacagg acctcacgac cctggaggcc ctggccttcg gcggggtatc cagcgccgaa
      541 gagcttgacc gcctggtggc cgaagtgcgt tccagtcgac ggctgagcga gaagcagaag
      601 ctgcacctga ccaacgccct gcgcgcccgg tacaccaagt ccagcggtac aaaatgatca
      661 ccccacagcg aacgaccatg aaacgcttcc tccttcgctc ccttcccgcc gtggcggtga
      721 tcggcctcac ggcgaacgtg aacgcccaga acaggggcgt ggcggtgaac gccaccggtg
      781 cagcccccaa cgacatggcc atcctggacg tgtcctccac caccaagggc atgctgatcc
      841 cgcgcatgac cctggcccag cgcaacgcga tcgcagcccc catcccgcag ggccttaccg
      901 tctatgtgga cgatgcggtg aacacgaacg acggtttctg gatccgcgag tacaacacat
      961 gggtacgctt ggacatcggt gccgatggct gggacatcaa cgggaacaac gtgggggcgc
     1021 ctgccacgga tttcttcggc acgcgcgaca acgtggacct ggtcttccgc accaacaacg
     1081 cgcaggtgat gcgggtgtcg ggcaccacgg tgaacaacgg gcgctacacg catatcggta
     1141 ccaacatcac ggcaccgaac gagcagttgg acatcggttc gggcagcccg agcacgggtg
     1201 cgctgcggat ctacaacccc gcgccaggta cctgggcggc caccaacccc atggtgaaat
     1261 atgcggccac caacacgcag ggcaccattc acttccggcc catcggcgcg ttgggcgcta
     1321 ccggtgactc actggaggcc accttcaacc cgcccggtta tcccacccag cagagtcgcg
     1381 atgtggtgat gtggaacggt cactggggga atatcaccgg cacagccccg gtgctgaaca
     1441 cggcgaccgc gcactccggc gggtggaaac gactggagaa cgagtacatc gagatcttca
     1501 acaaggcgtt cacccagcag gccacgcccg tttgcggcaa cacgccggtg gaactcaccc
     1561 agggctcaac aacctacacc agcggtttgc tcgccgctga cgcactgctg atcaatccgt
     1621 acaagttcga tatgccgggc aggcggtacc gggcacagta cctcttcctt cccgacgagt
     1681 tgaacgtgga acgcaaccag ttggtattgg gcaacttgaa cgcgacgggt ggcttgtgcg
     1741 ccggttcgcc catgaacagc atcggtttct acgtgaacat gaacgtcacc acccagaccc
     1801 gggcggccaa cgtcaccatc acggtgaaac acgcaccact gggcaccacc atgctcacgg
     1861 gttttgacga ttcggtggat ccagcgcaag gctgtttcaa caacgccgcg ctcaatccgt
     1921 tcccaacggc tggcggaccg gggtggaaga attacacgct caccacaccg ttcacgtggg
     1981 atggtgagcg gccggtgatc gtggaggtcg ccgtacacca ggccgccgcc ggtggcggtg
     2041 ggacccttcc gggcggattg cccatccggt attccaccac gggcacgacc accacgtaca
     2101 gtgcgtacac cacgttccct gcggggatcg gggcccccgg ttccccatgc aatccgaacg
     2161 ttcccacgtt cagcacct
//
LOCUS       DAOQOC010000487         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32134_length_2178_cov_3.037683, whole genome shotgun sequence.
ACCESSION   DAOQOC010000487 DAOQOC010000000
VERSION     DAOQOC010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32134_length_2178_cov_3.037683"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..321
                     /locus_tag="PL106_08450"
     CDS             184..321
                     /locus_tag="PL106_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33138.1"
                     /translation="MNAYIIAGFRSAVGKAPRGKFRFTRWNAVDGLWSVVYGCERGVL
                     G"
     gene            318..707
                     /locus_tag="PL106_08455"
     CDS             318..707
                     /locus_tag="PL106_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNA33139.1"
                     /translation="MNRYKNLEIWKDAIKLAKAVHVLTECLPKEETYGLKSQLRRAAV
                     SVASNIAEGAGRNTNGEFYQFLGIAIGSAFEVDTQLTIGVELGYFKADTVKEMGLQID
                     SLANRVNKLQQTLTPGKPRQGLSGQRP"
     gene            732..1907
                     /locus_tag="PL106_08460"
     CDS             732..1907
                     /locus_tag="PL106_08460"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNA33140.1"
                     /translation="MDAYIIAGFRSAVGKAPRGKFRFTRPDDLAAKVVHRLMASVPNL
                     APEQVDDVIVGNATPEAEQGLNIGRMISLMGLNTDKVPGMTVNRYCSSGSETIAIAAA
                     KIHSGQADVIIAGGVECMSPIPFGGWRIVPNYEVSKVNPTYYWGMGLTAEAVARDFKV
                     NREDQDAFALKSHEKALAAIAAGKFKDEIVPVEVERIFLDEKEKRQVEKYVVDTDEGP
                     RASTLEGLAKLKPVFDAKGATTAGNSSQTSDGAAFVLVVSERMLKALNVQPIARLLSY
                     HVAGVEPRIMGIGPVAAVPKALEKAGLKLNDIGLFELNEAFASQSLAVVRELGINPDI
                     VNVNGGAIALGHPLGCTGTKLSVQLFNEMRRRKQKYGVVTMCVGTGQGAASVFELLN"
     gene            1960..2109
                     /locus_tag="PL106_08465"
                     /pseudo
     CDS             1960..2109
                     /locus_tag="PL106_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991381.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
BASE COUNT          480 a          602 c          730 g          366 t
ORIGIN      
        1 aacacgaacc tcttgcatgt gtcatagaat aagggataga agcggaaagc ccgcaggcct
       61 gattgcgagg aggaacgacg aagcaacagg ccgaggactt gaagcggata gcccgacggc
      121 gctgctcttg agcgccggca cgcccaacac ttcgacaagc tcagtgtgac aacacgaacg
      181 aacatgaacg catacatcat cgccggtttc cgcagtgcag tgggcaaggc cccgcgcggc
      241 aaattccgtt tcacccgttg gaatgcggtc gatggtctat ggtcggtggt ctatggctgt
      301 gagagaggtg tgttaggatg aacagatata agaacctcga gatctggaag gatgcgatca
      361 agctcgcgaa ggctgtgcac gtccttacag agtgcttgcc gaaagaagag acctatggct
      421 tgaagagcca gttgcgtcgg gcggctgttt ccgttgcgag caacatcgct gagggcgccg
      481 gccgaaatac caatggcgag ttctatcagt tccttggcat cgcgataggc tctgcattcg
      541 aggtcgatac ccagctgacc atcggcgttg agctgggtta cttcaaagca gacacggtga
      601 aggagatggg cttgcagatc gacagcctcg ccaaccgcgt gaacaaactt caacaaacac
      661 tcactcccgg caagccacgt cagggcttgt ccggccagcg accatagacc aacgaccata
      721 gacgcactca catggatgca tacatcatcg ccggtttccg cagcgcagta ggtaaagccc
      781 cgcgcggcaa attccgtttc acccgtccgg acgacctggc cgccaaggtg gtgcaccgcc
      841 tgatggcgag cgtgccgaac ctggcgcccg agcaggtgga cgatgtgatc gtgggcaacg
      901 ccacgcccga ggccgagcag ggcctgaaca tcggccgcat gatcagcctg atgggcctga
      961 acaccgacaa ggtgccgggc atgacggtga accgctattg cagcagcggc agcgagacga
     1021 tcgccattgc cgcggccaag atccacagcg ggcaggccga cgtgatcatc gccggcggcg
     1081 tggagtgcat gagccccatc cctttcggcg gctggcgcat cgtgcccaac tacgaggtga
     1141 gcaaggtgaa cccgacctac tactggggca tgggcctgac cgccgaggcc gtggcgcgcg
     1201 acttcaaggt gaaccgcgag gaccaggacg ccttcgcgct gaagagccac gagaaggcgc
     1261 tggccgcgat cgccgccggg aaattcaagg acgagatcgt gccggtggaa gtggagcgca
     1321 tcttccttga tgagaaggag aagcgacaag tggagaagta cgtggtggac acggatgaag
     1381 gtccgcgcgc gagcaccctg gagggcctgg cgaaactgaa gccggtgttc gacgcgaaag
     1441 gcgcgaccac ggcgggcaac agcagccaga cgagcgatgg cgcggccttc gtgctggtgg
     1501 tgagcgagcg catgctgaag gccctcaacg tgcagccgat cgcgcgcctc ctctcctacc
     1561 acgtggcggg cgtggagccg cgcatcatgg gcatcggtcc ggtggctgcc gtgccgaagg
     1621 cgctggagaa ggccgggctg aagctgaacg acatcgggct gttcgagctg aacgaggcct
     1681 tcgcatcgca atcactggcg gtggtgcgtg agttgggcat caacccggac atcgtgaatg
     1741 tgaacggtgg ggccattgca ttgggccatc cactcggctg taccggtacc aaactctccg
     1801 tgcaactctt caatgagatg agaaggcgca agcagaaata tggagtcgtc accatgtgtg
     1861 ttggaaccgg acaaggggct gcgtcggtct ttgaactgct caactaacat gcggccactg
     1921 gaacacccat ctaagcgatc ctgccttcgg caggcaggcg cgctggggca tccgctgggg
     1981 tgcacgggca cgaagctgag cgtgcaactc ttcaatgaga tgcggcggcg gaagcagaag
     2041 tacggtgtgg tgacgatgtg cgtggggacg gggcaggggg cggcgagtgt ttttgagttg
     2101 ttgaattgat ggagtggaag cgcgatagtt gtgcccgccc tgagcgaagt cgaagggtgg
     2161 gcgtgccccc tcgcaaag
//
LOCUS       DAOQOC010000488         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32271_length_2173_cov_3.607177, whole genome shotgun sequence.
ACCESSION   DAOQOC010000488 DAOQOC010000000
VERSION     DAOQOC010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32271_length_2173_cov_3.607177"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1067
                     /gene="plsX"
                     /locus_tag="PL106_08470"
     CDS             120..1067
                     /gene="plsX"
                     /locus_tag="PL106_08470"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNA33141.1"
                     /translation="MRIGLDIMGSDHGPVVPIRAAVMAAREFPDVRLVLLGDAQAIAE
                     GLKGEGADPGSFDIVPSTDDITFNDNATKALGQKPKSSISLGFHLLKEGRLDAFASTG
                     NTGAMLVGSIFSIKPIEGVLRPCIPSILPKEGGGHGVLLDVGANADCKPDVLCQFGLL
                     GSLFAEYVYGIKDPKVALLNIGEEANKGNTATQAAYDLMKESGQFNFVGNIEGRDLFN
                     DKADVVVCDGFTGNIVLKAVESFHELLSARGVHDPFFDRLDYEGYGGLPVLGVNGNVM
                     IGHGISNAETIKNLVRTSRDVVNARLNERIRAAFQFQPQ"
     gene            1064..2056
                     /locus_tag="PL106_08475"
     CDS             1064..2056
                     /locus_tag="PL106_08475"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNA33142.1"
                     /translation="MRTTAAITAVQGTVPDHVLDNAQLEKLVDTNDAWITERTGIKER
                     RILKGKDQGLSVLAVEAVKGLCAKRGIDPMEIDLLICCTVTADLIFPATANIICDQVG
                     AKNAWGFDLNAACSGFLYGLTTGAQFIETGRHKKVVVVGGDKMSAIIDYTDRSTCIIF
                     GDGAGAVLLEPNNEGLGVLDSILRSDGSGCQFLHQKAGGSRMPTSIQTVEARQHYVYQ
                     EGKAVFKFAVTNMADVSAEIMAKNGLKSEDVAWLVPHQANKRIIDATAHRMGLDDSRV
                     MVNIEKYGNTTAGTLPLCLWEWEPRLKKGDNLILSAFGGGFTWGAVYVKWAYGG"
BASE COUNT          455 a          671 c          691 g          356 t
ORIGIN      
        1 cggcgacgag ctgatctacc gcggcaaggt ggtgtacacc aaggccaagc cggagtaacc
       61 ggatccgcat ttcatttgcc ccgcttctcc gcccaccagg gcagagttcc ccgcgcggta
      121 tgaggatcgg actcgacatc atgggcagcg accacggacc ggtcgtgccc atccgtgctg
      181 ccgtgatggc ggcccgggaa ttccctgacg tacggctggt cctcctgggc gatgcacaag
      241 ccatcgcgga aggactgaag ggcgagggtg cggacccggg atcttttgat atcgtcccca
      301 gtactgacga catcaccttc aacgacaacg ccaccaaggc gcttggtcaa aagccgaaaa
      361 gcagcatcag cctcggcttc cacctgttga aagaaggccg gttggacgcg ttcgccagca
      421 cgggcaacac cggggccatg cttgtaggca gcatcttcag catcaagccc atcgaaggcg
      481 tccttcggcc gtgcatcccg agcattttac ccaaggaagg cggcggccac ggcgtgctgc
      541 tggatgtggg agccaatgcc gactgcaagc ctgacgtgct ctgccagttc ggtttgttgg
      601 gctcgttgtt cgccgaatac gtttacggca tcaaggatcc caaagtggcg cttctcaaca
      661 tcggcgagga ggccaacaag ggcaacacgg ccacacaagc cgcctacgac ctgatgaagg
      721 agagcggcca gttcaacttc gtggggaaca tcgaaggtcg cgacctcttc aatgacaagg
      781 cggacgtggt ggtttgcgac ggtttcaccg ggaatatcgt actcaaagcg gtggaatcgt
      841 tccatgagct cctgagtgcc cggggtgtgc atgacccctt cttcgaccgg ctggactatg
      901 agggttacgg ggggctgccg gtgctgggcg tgaacggcaa cgtgatgatc ggccacggga
      961 tcagcaacgc cgagaccatc aagaacctgg tgcgtaccag ccgcgacgtg gtgaacgccc
     1021 ggctgaacga acgcatccgc gccgcgttcc aattccaacc acaatgagga ccaccgcggc
     1081 catcacggcc gtccagggca ccgttccgga ccatgtcctg gacaatgccc aactggagaa
     1141 actcgtggac accaacgatg cctggatcac cgagcgcacc gggatcaagg aacggcggat
     1201 ccttaaaggc aaggatcaag gattgagtgt gctggcggtg gaggccgtga agggtctttg
     1261 cgccaagcgc ggcatcgacc ccatggagat cgacctgctg atctgctgca ccgtcaccgc
     1321 cgacctgatc ttcccggcca ccgccaacat catctgcgat caggtgggcg ccaagaacgc
     1381 ctggggcttc gacctcaatg cggcgtgcag cggattcctc tacggcctca ccaccggtgc
     1441 gcagttcatc gagaccggca ggcacaagaa agtggtagtg gtcggtggcg acaagatgag
     1501 cgccatcatc gactacaccg accgcagcac ctgcatcatc ttcggcgatg gggccggggc
     1561 ggtgctgctg gagcccaaca acgaagggct cggcgtgctg gacagcatcc tgcgaagcga
     1621 tggcagcggc tgccagttcc tgcaccagaa ggccggcggt agccgaatgc ccaccagcat
     1681 acagaccgtg gaggcccgcc agcactacgt gtaccaggag gggaaggccg tgttcaagtt
     1741 cgccgtgacc aacatggccg acgtgagcgc ggagatcatg gccaagaacg gcctgaagag
     1801 tgaggacgtg gcctggctcg tgccgcacca ggccaacaag cgcatcatcg acgccacggc
     1861 ccaccgcatg ggcctggatg acagccgggt gatggtgaac atcgagaaat acggcaacac
     1921 cacggccggc accctgcccc tgtgcttgtg ggaatgggag ccccgattga agaagggtga
     1981 caacctgatc ctcagcgcct tcggcggggg attcacctgg ggcgccgtgt atgtgaagtg
     2041 ggcgtacggg ggatagacga gggtgctccg actcctcctg tgcgtcccgg cacatccgga
     2101 caccgcccag gcacgagagg gcgccccggt ggcccgatcc ccggaacttc tacattggcc
     2161 cgccctatcg gcg
//
LOCUS       DAOQOC010000489         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32329_length_2171_cov_3.200378, whole genome shotgun sequence.
ACCESSION   DAOQOC010000489 DAOQOC010000000
VERSION     DAOQOC010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32329_length_2171_cov_3.200378"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PL106_08480"
     CDS             complement(<1..422)
                     /locus_tag="PL106_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNA33143.1"
                     /translation="MTAVRDSIRANMDRPLTALYLLLLFAGWANIYSAAYNPDHPNLF
                     DTAMEYGNQSVWIVVCLVLGAAVMMVHGDFIKNMAVPAYAVSLLLLALVPVLGKEVGG
                     NTNWLQLGPVRIQPSEFAKFATALVLTRYLSGLQAFRG"
     gene            complement(419..>2171)
                     /gene="mrdA"
                     /locus_tag="PL106_08485"
     CDS             complement(419..>2171)
                     /gene="mrdA"
                     /locus_tag="PL106_08485"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNA33144.1"
                     /translation="DDTWTARAAYISERKVTVFPSRGLILDRTGELLVANTPVYDIMV
                     VPREVKEFDTLALAHLLGVTPKDVHDRLTKARAYSGYKPSEFEKQVTADQFAGISLHL
                     HKYPGFYAQSRTLRTYPERIGAHMLGYLSEVSQRKVEQDPYYRAGDVIGVGGLEQYYE
                     PALRGRRGVKYVVVDVHNNVQGKFMGGQYDTLGVEGKNLYTSIDARMQAYGERLMAHK
                     KGSIVALDPRTGGILALVTSPSYDPELLVGRVRNHNYGVLQRDSLNPLFDRALQAQYP
                     PGSIFKLVQALTALQEGVITPQTGFPCNKALVGCHNHPMATDVEHAIQYSCNPYFYQV
                     FKREIEQNKDPDRFKDAVLGLNEWEGYVRSFGLGERPRVDLPAAKGGRVPNTAFYDKR
                     YGKGGWAFSTIYSVSIGQGEVEVVPMQMANLAAIFANRGWYIDPHIVTAIGHPDSLTR
                     WERHKTKVDDHWFDLIAEGMRRVTEEAGGTARAARIPGVTVCGKTGTAQNPHGKDHAV
                     FVCFAPMENPRIAMAVYVENSGFGGTWAAPIASLLMEQYLTDTITRPDLEKRMLEADL
                     IADEKNYKPFVSKRRKR"
BASE COUNT          366 a          693 c          674 g          438 t
ORIGIN      
        1 atgcccctga aggcctgtaa tccgctcaag taccgcgtga gaaccagcgc cgtggcgaac
       61 ttggcgaatt cgctgggctg gatgcgtacc ggcccgagtt gcaaccagtt cgtattgccc
      121 ccgacctcct tgcccaggac gggcacgaga gcgagcagca ggagcgatac ggcgtaggcc
      181 ggaacggcca tgttcttgat gaagtcgcca tgcaccatca tcacggcagc cccgagcacc
      241 aggcacacca cgatccacac gctctggttg ccgtactcca tcgccgtgtc gaacaggttg
      301 ggatggtcgg ggttgtaggc ggcgctgtag atgttggccc agccggcgaa gagcagcaac
      361 aggtacaggg ccgtgagcgg ccggtccatg ttggcgcgga tgctgtcgcg tacggcggtc
      421 atcgcttgcg gcgtttgctg acgaaaggtt tgtagttctt ctcgtcggcg atcaggtcgg
      481 cctccagcat cctcttctcc aggtccggcc gcgtgatggt atcggtgagg tattgctcca
      541 tgagcaggct ggcgatgggt gcggcccagg tacccccgaa tccgctgttc tccacgtaca
      601 ccgccatggc gatcctggga ttctccatgg gcgcgaagca tacgaacacc gcgtggtcct
      661 tgccgtgcgg gttctgcgcg gtgcccgtct tgccgcacac ggtgactccc gggatccgtg
      721 cggcacgtgc ggtacccccc gcctcttcgg tgacccgtcg catgccttca gcgatgagat
      781 cgaaccagtg gtcgtccacc ttggtcttgt ggcgttccca cctggtcagg ctgtccggat
      841 ggcctatcgc tgttacgatg tgcgggtcga tgtaccagcc cctgttggcg aagatggccg
      901 cgagattggc catctgcatg ggcaccacct ccacctcgcc ctggccgatg ctcaccgaat
      961 aaatggtgct gaaagcccag ccacccttgc cgtaccgctt gtcgtagaag gcggtgttcg
     1021 gcacacgccc gcccttggcc gcgggcaggt ccacgcgggg tcgctcgccc aagccgaagc
     1081 tgcgcacgta tccctcccac tcgttcaggc cgagcacggc gtccttgaac ctgtccggat
     1141 ccttgttctg ctcgatctca cgtttgaaaa cctggtagaa ataaggattg caggagtact
     1201 ggatggcgtg ctccacatcg gtggccatgg gatggttgtg gcagccgacc agcgccttgt
     1261 tgcaggggaa gccggtctgc ggggtgatca caccttcctg gagcgcggtg agcgcctgca
     1321 cgagcttgaa gatggagccc ggtgggtact gggcctgcaa cgcacgatcg aacagcgggt
     1381 tgagtgaatc ccgttgaaga accccgtaat tgtggttgcg gacgcggccc accaggagtt
     1441 cgggatcata actcgggctg gtcaccagcg ccaggatccc gcccgtcctc ggatcaaggg
     1501 ccacgatact gcccttcttg tgggccatca ggcgttcacc gtacgcctgc atccgggcat
     1561 cgatactggt atacaggttc ttcccttcca cacccaacgt gtcgtactgg ccgcccatga
     1621 acttcccctg cacgttgttg tgcacgtcca ccaccacgta cttcacgccg cgccgtccgc
     1681 gcaaggccgg ctcatagtac tgctccaggc cgcccacgcc gatcacgtca cccgcccggt
     1741 aatacgggtc ctgctccacc ttccgctggc tcacttcgct caggtaaccc agcatgtgcg
     1801 ccccgatgcg ttccggatag gtgcgcaggg tgcggctctg cgcatagaac ccgggatact
     1861 tgtgcaggtg cagggagatc ccggcgaact ggtcggcggt gacctgcttt tcgaattcac
     1921 tgggtttgta gccggaatac gcgcgggcct tggtcagccg atcatgcaca tccttgggag
     1981 tgacgcccaa cagatgtgcc agtgccagcg tgtcgaattc cttcacctcg cgcggtacca
     2041 ccatgatgtc gtacaccggg gtgttggcca ccagcagttc gcccgtgcgg tcgaggatca
     2101 ggccgcgcga ggggaatacg gtgaccttgc gttcgctgat gtaggccgca cgcgccgtcc
     2161 aggtatcgtc c
//
LOCUS       DAOQOC010000490         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32504_length_2164_cov_4.539118, whole genome shotgun sequence.
ACCESSION   DAOQOC010000490 DAOQOC010000000
VERSION     DAOQOC010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32504_length_2164_cov_4.539118"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PL106_08490"
     CDS             complement(<1..404)
                     /locus_tag="PL106_08490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA33145.1"
                     /translation="MQYDPVKRQLGSVFNRSPPLRKFFYGLLDLLLLRAWHIHKELRA
                     WATDKKGRALTIYDAGAGFGQYSYWLSGLSPHWSISAVDVKEEQVADCNAFFQRIGRS
                     RVTFSVDDVTTHVRPGTYELVVCVDVMEHILE"
     gene            complement(406..747)
                     /gene="rbfA"
                     /locus_tag="PL106_08495"
     CDS             complement(406..747)
                     /gene="rbfA"
                     /locus_tag="PL106_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNA33146.1"
                     /translation="MDSLRQNKVNSLLLKEMAAILQTESHRLLPGGLITVTGVRVSPD
                     LGVARVYVSLFPVKDKAAALERVREDAHHMRGMLGRRVGKHMRVIPEILFYLDDSLDK
                     AEEIDKLLKGS"
     gene            803..>2164
                     /locus_tag="PL106_08500"
     CDS             803..>2164
                     /locus_tag="PL106_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA33147.1"
                     /translation="MHQFLRILRIGRPYRRFAVLNALFNLLGTVFHLLSLLLFIPFLR
                     LLLGQVKPVHERPADLWTKDGLENGFNWGLTRLIDVYGDMGALLTICIAVVVLFLCKN
                     LFRYLALLAITKFRNHIVRDIRQQVYGKLLELPLRYHSSERKGDLLALMTSDMHVIEF
                     SVMQYIEMVFREPIAVLMFLGTMVTLSPGLTAISLLMLPVSGLLIARVSRSLKKRSTR
                     VQEKDADLLARVEETLTGIRVIKAFNGEADMERRFARENGLLMRAHVSMMRRQDLASP
                     LSETLGAVVMAAIAYIGGSFVLGTQPSLSGDSFLGFIIIFSQLLAPIKGFSQGWSAIT
                     RGGASAQRIFDLLAVENTVKERPNAAPIEAFNHGIEFRDVTFAYDHTEVLRDIRITVP
                     KGRSVALVGTSGSGKTTLANLLPRFHDVTGGAVLVDGKDIRDLRITDLRALMGIVTQD
                     SILF"
BASE COUNT          391 a          689 c          681 g          403 t
ORIGIN      
        1 tcctccagga tgtgctccat cacgtccacg cacaccacca gttcgtaggt gccgggtcga
       61 acatgggtgg tcacgtcatc cacgctgaag gtcacccggg aacgaccgat gcgttggaag
      121 aaggcgttgc aatcggccac ctgctcctcc ttcacatcca cggcggagat gctccagtgg
      181 ggagatagcc cactgagcca gtagctgtat tggccgaagc cggcgcccgc atcgtaaatg
      241 gtgagtgcgc ggcccttctt gtcggtcgcc cacgcccgca actccttgtg gatgtgccag
      301 gcgcgcaaca gcagcaggtc cagcagaccg tagaaaaact tgcgcagcgg cggcgatcgg
      361 ttgaagaccg aaccgagctg gcgtttgacg gggtcgtact gcatcctacg accccttcag
      421 cagtttgtcg atctcctcgg ccttgtcgag cgaatcatcc aggtagaaga ggatctcggg
      481 gatcacgcgc atgtgcttgc ccacgcgccg cccgagcatg ccgcgcatgt gatgggcatc
      541 ctcgcgtacg cgctccagcg ctgcggcctt gtccttcacc gggaagaggc tcacgtacac
      601 acgggccaca ccgaggtcgg ggctcacccg cacgccggta acggtgatga gcccaccggg
      661 cagcaggcga tggctctcgg tctggaggat ggccgccatc tccttcagca ggaggctgtt
      721 caccttgttc tggcgcaaac tgtccatggg ccgcgaatgt aggtgcgcgg aaaaccatgc
      781 ccctacattc gcgccggacc ccatgcacca gttcctgcgt atcctccgca tcggccgccc
      841 gtaccggcgt ttcgcggtgc tgaacgcgct gttcaacctg ctgggcacgg tcttccacct
      901 gctgtcgctg ctgctcttca tcccctttct gcgcttgttg ctgggccagg tgaagcccgt
      961 gcatgagcgc ccggcggacc tctggaccaa ggacggtttg gagaacggct tcaactgggg
     1021 cctcacacgc ttgatcgatg tgtacggcga catgggggcg ctgctcacca tctgcatcgc
     1081 cgtggtggta cttttcctgt gcaagaacct gttccgctac ctcgcgctgc tggccatcac
     1141 gaagttccgc aaccacatcg tgcgcgacat ccgccaacag gtgtacggca aactgttgga
     1201 gctgcccctg cgctaccaca gttccgagcg taagggcgac ctgctggcgc tgatgacgag
     1261 cgacatgcac gtgatcgaat tctcggtgat gcagtacatc gaaatggtct tccgcgagcc
     1321 gatcgccgtg ctgatgttcc tgggcaccat ggtgacgctg agccccggcc tcacggccat
     1381 ttcgctgttg atgctgcccg tgagcgggtt gttgatcgcc cgggtgagcc gcagtttgaa
     1441 gaagcgcagc acgcgcgtgc aggagaagga cgctgacctg ctggcgcgcg tggaggaaac
     1501 gctcacaggc atccgggtga tcaaagcgtt caacggagag gcggacatgg agcggcgttt
     1561 cgcacgcgag aacggattgc tgatgcgggc ccacgtcagt atgatgcggc ggcaggacct
     1621 ggcctcaccg ctgagcgaga ccctcggcgc cgtggtgatg gcggccatcg catacatcgg
     1681 cggcagcttc gtgctgggca cgcaacccag tctcagcggc gacagcttcc tgggcttcat
     1741 catcatcttc tcgcagctgc tggcgcccat caagggcttc tcgcaaggct ggagcgccat
     1801 tacgcgtggc ggagcgagcg cgcaacgcat cttcgacctg ctggccgtgg agaacacggt
     1861 gaaggaacga ccgaacgccg cgcccatcga ggccttcaac cacgggatcg aattccgcga
     1921 cgtcacattc gcatacgacc atacggaggt gttgcgggat atccgcatca ccgtgcccaa
     1981 aggccgcagc gtggcgctgg tgggcaccag cggcagcggc aagaccacgc tggccaacct
     2041 gctgccgcgc ttccatgatg tcacgggcgg tgccgtactg gtggacggga aggatatccg
     2101 cgacctgcgg atcaccgatc tgcgggcgct catgggcatc gtgacgcagg acagcatcct
     2161 gttc
//
LOCUS       DAOQOC010000491         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32557_length_2163_cov_2.731973, whole genome shotgun sequence.
ACCESSION   DAOQOC010000491 DAOQOC010000000
VERSION     DAOQOC010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32557_length_2163_cov_2.731973"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="PL106_08505"
     CDS             <1..1752
                     /locus_tag="PL106_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA33148.1"
                     /translation="IFVVIHQPSSEIFKLFDRLLLMDQEGHPVYYGDPVDAVVYFKKV
                     TGQVNSDVGQCATCGNVNPEQIFDILEARIVDEHGNETDQRRIGPEAWNEVFRAQMEV
                     RTARVPDGTAVPRSTFRAPGKLKQMAVFFKRDLMSKLANRQYVLINLIEAPALALVMA
                     FFLRFNAAGGTNGSYVFRQNDNIPQYLFISVIVALFLGLTVAAEEIIRDRRITQREKF
                     LSLSRSSYLLSKIGLLFMISAVQTFLFAFTGHRVLGFHGLLAEHWAVFFTTACFANVL
                     GLNVSASFNSAKVIYIVIPVLIIPQLLFSGIIVRFDKLHPWFAGEKSVPWIGNIMASR
                     WAYEGLATAQYTDNDYERLFYRCDQRMKTANWKKDLWVRDLQDRSAALRRALRTGGST
                     APMAEDLALVRHELEKETHLLKGFVFSGMDKLAPDALDVAVLDELDSALNTLTSHYRN
                     TYRAAERDKEALIARMTATPELRSAYFRLLDEHRNESLADHVTNKNDVNLIVEDDGEL
                     VQKSDPIYLEPAEGGLLSAHFYAPVKWILGLRVHTLWANVLFIWAMSAALALALRFNV
                     FPVVVERLSRKPWNRGA"
     gene            complement(1772..>2163)
                     /locus_tag="PL106_08510"
     CDS             complement(1772..>2163)
                     /locus_tag="PL106_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNA33149.1"
                     /translation="ALHLEGTPKTPTVSFDDASGLLELKGRSIPENSIEYYKPLIDWV
                     DRYARDPRPRTTLHVQLEYFNTSSSKCILDVFKKLEMIRVTGNEVTVLWHYEQDDEDM
                     LEAGEDYRAIINIPFKMIQIEEVNGGK"
BASE COUNT          405 a          651 c          667 g          440 t
ORIGIN      
        1 attttcgtgg tgatccacca gcccagctcg gagatcttca agctgttcga ccggctgctg
       61 ctcatggatc aggagggcca cccggtgtac tacggcgacc ccgtggatgc ggtggtctac
      121 ttcaagaagg tgaccggcca ggtgaacagc gacgtgggcc agtgcgccac ctgcggcaac
      181 gtgaaccccg agcagatctt cgacatcctg gaggccagga tcgtggatga acatggtaac
      241 gagacggatc agcgtcgcat cggccccgag gcctggaacg aggttttccg tgcgcagatg
      301 gaggtccgta ccgcccgtgt gcccgatggg acggcggtgc cccggagcac gttccgcgca
      361 cccgggaagc tcaagcagat ggccgtgttc ttcaagcgcg atctgatgag caagctggcc
      421 aaccgccagt atgtgctcat caatctgatc gaagccccgg cccttgccct ggtgatggcc
      481 ttcttcctgc gcttcaacgc cgcgggaggc accaacggca gctacgtctt ccgtcagaac
      541 gacaacatcc cgcagtatct tttcatcagc gtgatcgtgg ccctgttcct cggcctcacg
      601 gtggcggcgg aggagatcat ccgcgacagg cgtatcaccc aacgggagaa gttcctgtcg
      661 ctgagccgca gcagctacct gctcagcaag atcggcctgc tcttcatgat cagtgccgtg
      721 cagaccttcc tcttcgcatt cacaggtcac cgggtgctcg gtttccatgg tcttctcgcc
      781 gagcattggg cggtcttctt caccacggct tgtttcgcca acgtgcttgg actcaacgtg
      841 agcgcgagtt tcaacagcgc caaggtgatc tacatcgtca ttccggtact catcatcccg
      901 cagttgctct tcagcgggat catcgtgcga ttcgacaagc tgcatccctg gttcgcgggg
      961 gagaagagcg tgccctggat cggcaacatc atggcgagcc gatgggccta tgagggtctg
     1021 gccaccgcgc agtacacgga caacgattat gaacggctct tctaccgctg cgatcagcgt
     1081 atgaagacgg cgaactggaa gaaggatctc tgggtgcgcg atctgcagga ccgcagtgca
     1141 gcgttgcgcc gggctctgcg cactggtgga tcaacggccc ccatggccga ggacctggcc
     1201 ctggtgcgac atgaactgga gaaggagaca cacctgctca aaggatttgt gttctcgggt
     1261 atggacaagc tggcgcccga tgcgctggac gtcgcggtgc tcgacgaact ggactccgca
     1321 ctcaacacgc tgacgagcca ctaccggaat acctaccggg ccgctgagcg ggacaaggaa
     1381 gcgctcatcg cgcggatgac ggccacccct gagctgcgtt cggcgtactt ccgcctcctg
     1441 gacgagcacc gcaacgagag cctggcggac cacgtgacca acaagaacga cgtgaacctc
     1501 attgtggagg atgatggaga actggtgcag aagagcgatc cgatctacct ggagcccgcc
     1561 gagggagggt tgctcagcgc gcacttctac gcaccggtga aatggatcct cggccttcgc
     1621 gtacacacct tgtgggccaa cgtgctgttc atctgggcca tgagcgccgc gctcgcgttg
     1681 gccctgcgct tcaacgtgtt ccccgtagtg gtggagcggc tgagccgcaa gccgtggaac
     1741 aggggcgcgt gaggtgcggt gcccgtgccg cctactttcc gccgttcacc tcctcgatct
     1801 ggatcatctt gaaggggatg ttgatgatgg cgcggtagtc ctccccggcc tcgagcatgt
     1861 cctcgtcgtc ctgctcgtaa tgccagagca cagtgacctc gttccccgta acgcgtatca
     1921 tttcaagctt cttgaagacg tcgaggatgc acttgctgct gctcgtgttg aagtattcca
     1981 actgcacatg cagcgtggtc ctcgggcgcg ggtcacgcgc gtaccggtcc acccaatcga
     2041 tgaggggctt gtagtattcg atgctgttct caggaatgct gcgtcctttg agttcgagca
     2101 gtcccgaggc atcatcgaac gatacggtgg gtgttttcgg ggtcccttcg agatggagag
     2161 ctg
//
LOCUS       DAOQOC010000492         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32569_length_2162_cov_4.676317, whole genome shotgun sequence.
ACCESSION   DAOQOC010000492 DAOQOC010000000
VERSION     DAOQOC010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32569_length_2162_cov_4.676317"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PL106_08515"
     CDS             <1..1215
                     /locus_tag="PL106_08515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA33150.1"
                     /translation="KNATASPYTEGTADHLNDSLITHADTGVVVGPFREGDLWKLVKV
                     KQLADVPEARVRHILFSTQGKSEGEQTAIKNRADSVLAVVKRDRSKFEAMVTKYTEDP
                     GSKSTGGVYEWFDKTRMVPEFTAASFDQKPGAITICKTSYGYHIVEVLGQRNRQERRV
                     LTVDRKVRPDRALKQAWQTASAFGLNNTDTASMHKAATEAGLTYTPVGELRSDQRFVP
                     GLQDPSGVISYVERAEPGGKPSEPLLSGEQYVVALLTGIREEGDPRFEDVKEAMTGEV
                     RKGKKAEALIAKFTGKSDLNALATEQSLTVQSANDMAMSAFSIPGGYNDPEVVGRIFA
                     VAPGQTSAPLKGDMAVYVANMTTLTPAGEQPADPNAAKPVADRFRNRAEGQAFNALKE
                     AAGVKDDRSRFF"
     gene            1523..1666
                     /locus_tag="PL106_08520"
     CDS             1523..1666
                     /locus_tag="PL106_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33151.1"
                     /translation="MILLLFLAAVTAMIPSILITGNDTPPPFPVAGDDMLLAADRQDA
                     LRY"
     gene            1746..>2162
                     /locus_tag="PL106_08525"
     CDS             1746..>2162
                     /locus_tag="PL106_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33152.1"
                     /translation="MANRIIPILAALAFATAAMSFLLPCFMVGMGTAGMIPIHGSALV
                     TGTPPVVPAVPVMDTVATSEAMPGGREEAGTSSADPTSRARQFLRNRTATTSTGSDTP
                     APDTRTSTLSARSPTAVAILVLVITGLVASLMPQRIL"
BASE COUNT          440 a          698 c          668 g          356 t
ORIGIN      
        1 aagaacgcca ccgcttcgcc gtacaccgaa ggcaccgccg accacctgaa cgactccctc
       61 atcacccatg ccgataccgg agtggtggta ggtccgttca gggagggcga tctctggaag
      121 ctcgtgaaag tgaagcaatt ggccgacgtt ccggaagccc gtgtgcgcca catcctcttc
      181 agcacccagg gcaagagcga gggcgagcag acggccatca agaaccgcgc ggacagcgtg
      241 ctggccgtgg tgaagcgtga tcgctcgaag ttcgaggcca tggtgacgaa atacaccgag
      301 gatcccggta gcaagagcac cggaggcgtg tacgagtggt tcgacaagac ccgtatggtg
      361 cccgagttca cggcggcgag cttcgaccag aagccggggg ccatcaccat ctgcaaaacg
      421 agttacggct accacatcgt ggaagtgctg ggtcagcgca accgccagga gcgccgggtg
      481 ctcaccgtgg accgcaaggt gcgccctgac cgcgcactga agcaggcatg gcagacggcc
      541 agcgcgttcg gattgaacaa taccgatacc gcttccatgc acaaggccgc caccgaggcc
      601 ggcctcacct acacaccggt cggcgaacta cggagcgatc agcgatttgt ccctggactg
      661 caggatccca gcggcgtgat ctcctacgtg gagcgcgcgg aacccggtgg caagcccagt
      721 gaaccgttgc tcagcggcga gcagtacgtg gtggctttgc tcaccggcat ccgcgaggaa
      781 ggagaccccc gattcgagga cgtcaaagag gcgatgaccg gggaggtacg caaagggaag
      841 aaggccgagg ccctgatcgc caagttcacc ggcaagtccg acctgaacgc cctggccacc
      901 gaacagagcc tcacggtaca gagcgccaac gacatggcca tgagcgcatt cagcatcccc
      961 ggcggttaca atgatcccga agtggtggga cgcatattcg ccgtggctcc cggacagacc
     1021 agtgcacccc tcaaaggcga catggccgtg tacgtggcga atatgacgac cctgacacct
     1081 gctggcgagc agcctgccga tcccaatgcc gccaagcccg tggccgaccg cttccgcaac
     1141 cgtgccgaag gccaggcctt caatgccctg aaggaagctg ccggtgtgaa ggacgatcgc
     1201 agccggttct tctgatcaac accaggatat cgatcgagcg gggcgctgga agcgccccgc
     1261 tctcttttgt cacgagccgg aaccgcgaga acaggttgtt cgccctgatc gggtcgttcg
     1321 gctttgtgcc gacgggtccg gggccgtggg gggtcaagct gccgtggtag ttcatcaccg
     1381 cgacagacct ttttcattcc actgggcgat cgggacggag ttgcatggat cgtgggatac
     1441 acgcgatccg cacgggcata cgctcatccg cgcggggatc ggcccgaccg tggaccgagt
     1501 attgcgcctt ataccgcgca ccatgatcct cctgctgttc cttgctgccg tcacggccat
     1561 gatcccgtcg atcctgatca ccgggaacga cacacctcca ccattccctg ttgctgggga
     1621 tgatatgctc ctcgctgccg accgccagga tgccctcaga tactgagcgt gtggagcgac
     1681 cggtacgcgg cgaatacccc taccttcacc cgccttcgac tccaaaggag cgcggaaacg
     1741 accaggtggc gaaccgcatc atcccgatcc ttgcggcctt ggccttcgcc acggcggcca
     1801 tgagttttct tctgccctgc ttcatggtgg gcatggggac cgcaggaatg atacccattc
     1861 acggcagcgc cctggtgacg ggtactccac cggtggtgcc cgcagtgcct gtgatggata
     1921 cggtagccac ctccgaagcc atgcccggtg ggcgggaaga agcagggacg tcctcggcgg
     1981 atccaacgtc gcgggcccgg caattcctgc gcaacagaac agcgacgacc agcacgggtt
     2041 cggatacccc tgcgccagat acacggacat caacgctgag cgcgcgatca cccacggccg
     2101 tcgccatcct cgtcctggtc atcacgggac ttgtggcctc gttgatgcca caacggatcc
     2161 tt
//
LOCUS       DAOQOC010000493         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32621_length_2161_cov_2.884141, whole genome shotgun sequence.
ACCESSION   DAOQOC010000493 DAOQOC010000000
VERSION     DAOQOC010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32621_length_2161_cov_2.884141"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PL106_08530"
     CDS             <1..771
                     /locus_tag="PL106_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33153.1"
                     /translation="VHAGGDRWPVGARFAGMGNAGLTLVDLWSVSANQAGLAGLEHPV
                     AGAYYQQHWLSSDLAMQGLAFAMPLGKGCIAVNGGSFGSTGLFAQQRYGLAYAMRLGQ
                     RLRVGVQLDYLNLRFGENYGKTGTVTAEVGLQAKLTDHLWIAAHLYNPNRARLGGPYN
                     ERVPTVFSAGLGYTFSERVVLCAQVDKDIDLPERYHVGIEYRPANVLYVRAGVSSRPV
                     QGHGGVGVRIKGLEIDLAVAARSQLGITPMFNLNYRFE"
     gene            768..>2161
                     /locus_tag="PL106_08535"
     CDS             768..>2161
                     /locus_tag="PL106_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNA33154.1"
                     /translation="MKRVAVLALACLLVRSACAQDPPGRASDEVDPQNDLRDIIEQRI
                     EAVADQMGSDNNVDLTALTEVLTDYIRSPMDLNKAEPQDLAQLQLLSDVQVTAIIEHR
                     QRFGPFISIYELQTIDEMDPRTIALIRPFVQVRADGTRSRASFKEILKNGSHEILSRT
                     IVNVEQRAGFRGQRNPFGVYYTDPDGGALPDTENAHITDSLRRANRLYLGDPFKIYAR
                     YRFRYRQNVSFGVTMEKDEGEQFFKGTQPNGFDFYSAHLFLRDVGPVKSLALGDYTAQ
                     FGQGLVFWSGLAYGSKSAYTMNIKRNAAGLMPYSSVNENLFLRGVATTVRMGKRLEGT
                     AFFSHKGIDANVTDSDVPLDENIDQEAAFSSLLEDGYHRTYQEVSRKDAVQETILGGH
                     LRYKRPRWSVGGTVAHVAYSHTLDRNLSVYNRFELEGRENTTMGVDWNVMYRNLTWFG
                     EGARSANGGMAGTTG"
BASE COUNT          412 a          676 c          719 g          354 t
ORIGIN      
        1 gtgcacgctg gcggcgaccg ctggcccgtg ggcgcgcgct tcgcgggcat gggcaacgcg
       61 ggcctcaccc tggtggacct ctggagcgtg agcgccaacc aggccggact ggccgggctg
      121 gaacaccccg tggccggcgc atactaccag cagcattggc tcagcagcga cctcgccatg
      181 caggggctcg ccttcgccat gccactgggc aagggctgta tcgcggtgaa tggcggttcc
      241 ttcggcagca cggggctgtt cgcgcaacag cggtacgggc tggcgtatgc catgcggttg
      301 ggccagcggt tgcgcgtggg cgtgcaactg gactacctga acctgcgctt cggtgagaac
      361 tacggaaaga cgggtaccgt taccgccgag gtgggcctgc aggcgaagtt gaccgaccac
      421 ctctggatcg cggcccatct gtacaacccc aaccgtgcca ggctgggcgg accgtacaac
      481 gaacgcgtac ccaccgtgtt cagcgcgggc ctgggctaca ccttcagcga gcgcgtggtg
      541 ctttgcgcgc aggtggataa ggatatcgac ctgcccgagc gctaccatgt gggcattgaa
      601 taccgcccgg cgaacgtgct gtacgtacgg gcgggcgtga gcagcaggcc cgtgcagggg
      661 cacggcggtg tgggcgtgcg catcaaaggc ctggagatcg acttggcggt ggcagcacga
      721 tcgcaactgg gcatcacgcc gatgttcaac ctgaactacc gcttcgaatg aaacgcgtgg
      781 cggtactggc cttggcgtgc ctgctggtgc ggtcggcctg cgcccaggac ccaccgggcc
      841 gcgccagcga cgaggtggat ccgcagaacg acctgcgcga catcatcgaa cagcgcatcg
      901 aggcggtggc cgatcagatg ggcagcgaca acaacgtgga tctcacggcg ctcaccgagg
      961 tactcaccga ctacatccgg tcgccaatgg acctgaacaa ggctgaacct caggacctgg
     1021 cgcagctgca actgctgagt gacgtgcagg tcaccgccat catcgagcat cgccagcgtt
     1081 tcgggccctt catcagcatt tacgagctgc agaccatcga cgagatggac ccgcgtacga
     1141 tcgcgctgat ccgtcccttc gttcaggtac gtgcggatgg cacccgctcg cgcgcctcgt
     1201 tcaaggagat cctcaagaac gggtcgcacg agatcctttc gcgcaccatc gtgaacgtgg
     1261 agcagcgggc cggcttccgg ggacagcgca accccttcgg tgtttattat accgaccccg
     1321 acggcggcgc gttgcccgac acggagaacg cccacatcac cgactccctg cgccgcgcga
     1381 acagattgta tctgggcgac cccttcaaga tctacgcccg ttatcgtttc cgctaccggc
     1441 agaacgtgag tttcggtgtc accatggaga aggacgaggg cgaacagttc ttcaagggca
     1501 cgcaacccaa cggcttcgac ttctacagcg cgcacctgtt cctgcgcgac gtgggcccgg
     1561 tgaagtccct cgccttgggc gactacaccg cgcagttcgg gcagggactc gtgttctgga
     1621 gcgggctggc ctacggcagc aagagcgcgt acaccatgaa catcaagcgc aacgcggcag
     1681 gtctgatgcc ctactccagc gtgaacgaga acctcttctt gcggggcgtg gccaccacgg
     1741 tacgtatggg caagcgactg gaaggcacgg cctttttcag ccacaagggc attgatgcca
     1801 atgtgacgga cagcgatgtg cccctggacg agaacatcga ccaggaggcg gcattcagtt
     1861 ccctgctgga ggacggctac caccgtacct accaggaggt ttcgcgcaag gatgcggtgc
     1921 aggagacgat ccttggtggg cacctccggt acaagcggcc gagatggagc gtgggcggca
     1981 ccgtggccca tgtggcgtac agccacacgc tggacaggaa cctgtcggta tacaaccggt
     2041 tcgagttgga ggggcgggag aacaccacca tgggggtgga ctggaacgtg atgtaccgga
     2101 acctcacctg gttcggcgag ggcgcgcgca gtgcgaacgg cggcatggcc ggcaccaccg
     2161 g
//
LOCUS       DAOQOC010000494         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_32678_length_2159_cov_2.902091, whole genome shotgun sequence.
ACCESSION   DAOQOC010000494 DAOQOC010000000
VERSION     DAOQOC010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32678_length_2159_cov_2.902091"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PL106_08540"
     CDS             complement(<1..187)
                     /locus_tag="PL106_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNA33155.1"
                     /translation="MHKLSWIMLAFFSRAVLPIQAGMNARLGKAATSPVHAAMLSFIV
                     GTAGLLLFLLITQRSVSW"
     gene            complement(371..1147)
                     /locus_tag="PL106_08545"
     CDS             complement(371..1147)
                     /locus_tag="PL106_08545"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33156.1"
                     /translation="MAPHRLLSCLVIPIASIVQAQYCSPTFANGCFNWYNQQVTIGDI
                     DLPIGSAECGTSDHTALSTVMEPGVAEPMTVISGVWTGCAVWVDLNNNMTFEDSENLF
                     HNYVGGDPSFTYTFDLTLPPGTPDGNYRMRVIAPWGSDGFTPGSSNGFGPCGSYQYGN
                     FDDFTLTVGTGTGEASHDAYPVRIRPNPTMDQVSISSADGRPIQRILVVGADGRTVLN
                     TPAAPNSGTTGVDLSALPAGTYAITLVDGAGTRTARILKL"
     gene            complement(1451..>2159)
                     /locus_tag="PL106_08550"
     CDS             complement(1451..>2159)
                     /locus_tag="PL106_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNA33157.1"
                     /translation="GSKDVHKVRNLERQLKTHFAFTRVPGGAEICERQLLTLIQKIKE
                     VEVDEEGIERFMDAVQLDLAGFDRDELIKRVVSLEFNRFLEQYREARDLNVDMARKDH
                     NPNARTREKKEFTRGTGEENMRSMFINLGSADGFEKGKMLGYLCGITGLTGQHFGRIL
                     LKDMYSFVDVDARHFDEVLGHFKGANYKGRKIRVDEGTGGHGGPPRGDRPNSSYSDKP
                     RASSFRPGGKKFHKERY"
BASE COUNT          387 a          661 c          674 g          437 t
ORIGIN      
        1 cccaggagac gctccgttgg gtgatcaaca gaaagagcag tagaccggcg gtacccacga
       61 tgaaggaaag catggccgca tgcaccggac tggtcgcggc tttgccgagt cgtgcgttca
      121 tgcccgcctg tatgggcagg acagctctgg agaagaaggc aagcatgatc caactgagtt
      181 tgtgcatggg cgatgggatg tggccaaatg tgcgacgatc gaagcgatcc gcgatccctc
      241 ggccaggtcc cacccaggac ttcacccgcg acgcatcctt gcccgtcttg ttccttccaa
      301 ggcagaataa tcccgatgat cgggaactct gatggacctg gtcgccaggc ggtgccgggg
      361 cgggattgcg ctacaacttc aggatacgcg cggttcgggt acccgcgcca tcgaccagcg
      421 tgatcgcgta ggtgcctgcg ggcagcgccg aaaggtccac gccagtggtt ccactattcg
      481 gtgcggctgg cgtgttcagg acggtgcggc catcggcacc caccaccagg atccgttgga
      541 tcggcctgcc atccgcactc gagatgctga cctgatccat ggtggggttg ggccggatac
      601 gcacgggata ggcatcgtgc gatgcctcgc cggtacccgt gcccaccgtg agcgtgaagt
      661 cgtcgaagtt gccgtactgg tagctcccgc acgggccaaa gccattcgaa ctgcccgggg
      721 tgaacccgtc ggaaccccag ggtgcgatca ctcgcatccg gtagttgccg tcaggagtgc
      781 cgggcggaag ggtgaggtcg aacgtgtacg tgaagctcgg gtcgccaccc acgtaattgt
      841 ggaacaggtt ctcgctgtcc tcgaaggtca tgttgttgtt caggtccacc cacaccgcgc
      901 aaccggtcca gaccccgctg atgaccgtca tgggctcggc cacaccgggt tccatcacgg
      961 tactcaatgc ggtgtggtcc gaggtgccgc attcagccga gccgatgggc aggtcgatat
     1021 cgccgatggt cacctgctgg ttgtaccagt tgaagcaacc gttggcgaag gtgggcgaac
     1081 agtactgggc ctggacgatc gaagcgatgg ggatcaccaa gcaggagagg agtcgatgtg
     1141 gtgccatggg tacgggagtt cgtgtgaacg aagttactga ctatagactt gtcccggttc
     1201 aagggtgcga gggtgcgaga gacgtgactc tcgcaccctc gcattttcgc acccccatgg
     1261 ttcattctgg cgacgacggt cgagagatcg gatcttcatc accggcatag caggtgttgt
     1321 ggatggggtt tcagcattgc gcattccccc ttgcgcggag cgcccgccgc gcccggatcc
     1381 cgggcggcaa ggccgcgacg ggtgagctgc tgggccacaa cgatcatacg gcccctgttg
     1441 cgagcgccgg tcagtaccgt tccttatgga attttttacc gccgggccgg aaggacgatg
     1501 cccttggctt gtcgctgtag gaactgttgg gccggtcgcc gcgcggtggg ccaccatggc
     1561 caccggtccc ttcgtccacc cggatcttcc gccccttgta gttggcgccc ttgaaatgcc
     1621 ccagtacctc gtcgaaatgt cgcgcgtcca cgtccacgaa cgaatacatg tccttcagga
     1681 ggatacggcc gaagtgctgc cccgtcagac ccgtgatccc acagaggtaa ccgagcatct
     1741 tgcctttctc gaatccgtcg gcgctgccca ggttgatgaa catggagcgc atgttctcct
     1801 cacctgttcc acgtgtgaac tccttcttct cccgcgtgcg cgcgttggga ttgtgatcct
     1861 tgcgggccat gtccacgttc aggtcgcgtg cttcgcggta ctgttccagg aaccggttga
     1921 actcgagtga gaccacacgc ttgatcagct cgtcccgatc gaaaccggcc aggtccagct
     1981 gcacggcgtc catgaaccgc tcgatacctt cctcatccac ttccacttcc ttgatcttct
     2041 ggatcaaggt gagcagctgc cgctcgcaga tctcggcccc gccgggcacg cgcgtgaacg
     2101 cgaagtgcgt cttcaactgg cgctccaggt tgcgcacctt gtgcacgtcc ttgctgccg
//
LOCUS       DAOQOC010000495         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33046_length_2146_cov_2.900048, whole genome shotgun sequence.
ACCESSION   DAOQOC010000495 DAOQOC010000000
VERSION     DAOQOC010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33046_length_2146_cov_2.900048"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PL106_08555"
     CDS             <1..1415
                     /locus_tag="PL106_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33158.1"
                     /translation="VATGQGPFTIASGKAGLVRLSVCGDDQGLFLVLEGVDQDFVEAH
                     GASGDVVFGTRTPVDPLVRREADHALNELTALGELWRIDGRSAAVLGMVQAAYGALDP
                     GTASFEPRSGRYRPLFGAHANDTASDPGSRALAWYLGTADAQQRIGALADSLLRDSAD
                     IERALAELDARNASAFTGEMRIGYVRAELSHVRQEFLQRLFNQAPRTVTSKAGLPGPQ
                     GRVLPSGLDPFLRKYLVGDTIRVPRAKHLIDHVITVPKGYGLVLEKGARLNMTAGSGI
                     IVHGALHARGTQLNPVFIRPADEGAPWAGIHVTGTGSTRCVLTGLRMSGGGASIGGEG
                     AVNAMLAFQGCEVRMSGCAINGSGAGAAIDVHHGKLSMKETWLAGGQVPQLNITFADL
                     EAEGCTFVGEGCPTGVLARGARITLSDLSFSGYSGTALDLALRSHVQAVRVGFTGNGK
                     DKSMDATSTWESGGSAVLIQ"
     gene            1610..>2146
                     /locus_tag="PL106_08560"
     CDS             1610..>2146
                     /locus_tag="PL106_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33159.1"
                     /translation="MIGCVLLSSISFCTAFAQAPSSEIVEAVVVYGDHKLIEQDEVLT
                     DEELSTMLDSLCTLDPAPQDLIRDLRLFQRIRRMDQSQMITLIDSLFELDTVPYALVN
                     EINLYADQMPTRQAVEESGVQTWSHHVDEPGYELYGEWNTVSPNAYGVELSSADSILQ
                     LRLCDPGQDCGFSMPVPPI"
BASE COUNT          389 a          610 c          722 g          425 t
ORIGIN      
        1 tcgtcgctac cggacaaggc ccgttcacca tcgccagcgg caaagcgggc ctggtgcgcc
       61 tcagcgtttg cggcgatgat caaggccttt tcctcgtgct tgaaggggtc gatcaggact
      121 ttgtggaagc gcatggggcg agcggcgatg tggtctttgg cacgaggaca ccggtggatc
      181 cactcgtgcg gcgcgaagcg gatcatgcgt tgaacgagct taccgcgctg ggcgagctgt
      241 ggcggatcga tgggcggtcc gctgccgtgc tgggcatggt acaggccgcc tatggcgcgc
      301 ttgatcccgg aacggcttca ttcgagccgc gctcgggcag gtacaggccg ttgttcggag
      361 cgcatgcaaa tgatacggcg agcgacccgg gaagccgggc cttggcgtgg tatctcggca
      421 ccgccgatgc gcagcagcgg atcggtgctt tggccgattc cctgcttcgc gatagcgctg
      481 acatcgagcg ggcactcgcc gaattggatg ccaggaacgc atcagccttc acaggcgaga
      541 tgcggatcgg ctatgtgcgc gccgaactca gccacgttcg ccaggaattc ttgcaaaggt
      601 tgttcaacca ggcgcctcga acggtcacca gcaaggccgg gctccccggg ccgcagggtc
      661 gggtgcttcc atccggattg gatcccttcc tgcgcaagta cctcgtgggc gataccatcc
      721 gtgtgccgcg cgccaagcat ttgatcgatc atgtgatcac ggtacccaag ggctatggcc
      781 tggtgctgga gaagggcgcc cggctcaaca tgaccgcggg cagtgggatc atcgtccatg
      841 gtgcgctgca cgcccggggt acccaactca atccggtatt catcaggcct gcggacgagg
      901 gcgcgccgtg ggcggggata catgtgacgg gtacgggcag cacccggtgc gtgctgaccg
      961 gcctccgaat gagcggggga ggtgcatcca tcggggggga gggggccgtg aatgccatgc
     1021 tcgcattcca aggttgcgag gtgcgtatga gcggttgtgc gatcaacggg tccggcgcgg
     1081 gagcagcgat cgatgtgcat catggaaaac tgtccatgaa ggagacctgg ttggccggtg
     1141 gacaagttcc gcaattgaac atcacgttcg ccgatctgga ggcggaggga tgcaccttcg
     1201 ttggtgaggg atgcccaaca ggcgtgctgg cccgaggcgc acggatcacc ctctccgacc
     1261 tatccttcag cgggtacagc gggaccgcgc tggacctggc gttgcgttca cacgtgcagg
     1321 ccgtgcgggt cggctttaca ggcaacggca aggacaagtc gatggacgcc acgagtacgt
     1381 gggagtcggg tgggtccgcg gtcctgatac agtagggtaa gtcggaagcc accggcccgg
     1441 ttgcacagcc aagcaaatcg ggtgaattcg gctggggatc cgtcggtttg ttcgtcgttt
     1501 tcaacaccgt cttgttggta agaggttcgc ggatatcttg cgcgctttct ttttcgtaga
     1561 aggccccgtg aacaggcaac ctgcacccca tttcggcgta cgtacacgct tgatcggctg
     1621 cgtcctgctc tcatccattt cgttctgtac ggccttcgcg caagccccgt catcggagat
     1681 cgttgaggcc gtggtggttt acggggacca caaattgatc gagcaggacg aggtcctcac
     1741 cgatgaggag ttgagcacca tgctcgattc gttgtgtacg ctggatccag caccccagga
     1801 cctgatccgc gacctgcgct tgttccagcg catccgtcgg atggaccagt cacagatgat
     1861 caccctgatc gattcgttgt tcgaattgga cactgttccc tacgccttgg tgaacgagat
     1921 caacctgtac gcggaccaga tgccaacgcg gcaggcggtg gaagagagcg gtgtgcagac
     1981 ctggagccac cacgttgatg aacccggata cgagctgtac ggcgaatgga acacggtgag
     2041 tccgaacgcg tacggggtcg agttgtcctc ggccgattcc attctgcagt tgcggttgtg
     2101 cgatcccggg caggactgcg gattcagcat gccggttcca ccgatc
//
LOCUS       DAOQOC010000496         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33164_length_2142_cov_2.548155, whole genome shotgun sequence.
ACCESSION   DAOQOC010000496 DAOQOC010000000
VERSION     DAOQOC010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33164_length_2142_cov_2.548155"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2142
                     /locus_tag="PL106_08565"
     CDS             <1..>2142
                     /locus_tag="PL106_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33160.1"
                     /translation="ELVARIHPDYTADRERLQRDVDDPEERSAALHGLELMLVDSIGA
                     ETDRQLLRLERDTTDKAQVLARVDRLRRLKEDHLREADEVLASAGQRYEASESRTVED
                     LILLNDQPTEGSADVDRAVASPTPHNDDYVRIRTEPDMVYSSTMDHRSTEKGFSTAIR
                     DRDRDMEVLTGLEEKIDSLETVLESMPVGRDFTKLRDRTDKLIDQHMVLRMEMGQRME
                     FITRSEYKAAKDSLTLLAAQNNVKGLAPSEPMAQLATSYEQEARRVMDEAAAKRKQAD
                     RIELVVVRDSLFRTTYVEELNALRLMDRAQTIRSYMQRRDFQRGERISYEEVEARMFG
                     PVIGAPAVVAQVLDEPDTSTTGRAVVPGTEPARVEGSVEVAVNKVSVDADPANAEARA
                     ARLKAGELETASLIAADRVNTLRDSIPKARRSDRDELEREVLRQTALSDSLHRASLAA
                     GEEAARLEQEATAAREAKAFQDRLMRYYYLDDQEMALVLNEEDRSRYFQARAKALAQQ
                     DEAARAHEQAAASKALADTIQAQSRAMLTAKDTGAAAVDAEVMRRAALLSDQAVRLNL
                     RSDSLERVARRMELAASANAAQAATILQGLSPERGTVIMAMEQRTRRVEPVLAEARSL
                     QAAESVRDTSGGTRDGLAQAPVRTGSRSSATDTVLVSTTKEQVAQNAGQGNVPAPSAG
                     GIATTPTSMSTSQPSTGSVDAAITPAAPALLP"
BASE COUNT          449 a          660 c          706 g          327 t
ORIGIN      
        1 cgagctggtg gcgcgcatcc atcccgacta caccgccgac cgcgaacgtt tgcaacgcga
       61 cgttgatgat ccggaggaac gcagtgccgc gttgcatggg ctcgaactga tgctggtgga
      121 cagtatcggt gccgagaccg atcgccaatt gttgcgactg gagcgggata ccacggacaa
      181 agcacaggtg ctggccaggg tcgacaggtt gcgcaggttg aaagaagacc acctccgtga
      241 ggcggacgag gtgctcgcca gtgctggtca acgctacgag gcttcggaat cgcgtacggt
      301 cgaagacctg atcctgttga acgaccagcc caccgaagga tcggcggacg tggacagggc
      361 cgttgcgagc cccacaccgc acaacgacga ttatgtgcgg atccggactg aaccggacat
      421 ggtctattcc agcaccatgg accaccgctc caccgagaaa ggcttcagca cggccatccg
      481 cgatcgcgac cgtgacatgg aggtcctcac cggtctggag gagaagatcg attcccttga
      541 aaccgtactg gagagcatgc cggtgggccg ggacttcacc aagttgcgcg atcgaacgga
      601 caagctgatc gatcagcaca tggtgctgcg catggagatg ggccagcgca tggaattcat
      661 aacccgttca gagtacaagg cggcgaagga cagcctgacc ctgcttgccg cacagaacaa
      721 tgtcaagggc ctggcaccgt ctgaaccgat ggcccagttg gccacctcgt acgaacaaga
      781 ggccaggcgc gtcatggacg aggccgccgc caagcgtaaa caggccgatc gcatcgagtt
      841 ggtcgtggta cgcgacagcc tcttccgcac cacctatgtg gaggagctca acgcccttcg
      901 cctcatggac cgggcgcaga ccattcgcag ctacatgcaa cggcgcgact tccagcgtgg
      961 cgagcggatc agctacgagg aggtggaagc gcgcatgttc gggccggtga tcggagcacc
     1021 agcagtggtc gcacaggtgc tcgacgagcc ggacacgagc accactgggc gcgcggtcgt
     1081 accaggaacg gagcctgcca gggtggaggg ctcggtggag gtcgctgtga acaaggtctc
     1141 cgtggatgcg gacccggcaa atgccgaagc gcgtgcggcc cggttgaaag ccggtgaact
     1201 cgagacggcc tcactgatcg ctgccgaccg ggtgaacacc ctgcgcgaca gcatccccaa
     1261 ggccaggcgg agcgaccggg atgagctgga acgggaagta ttgcgtcaga ccgcactctc
     1321 cgattcgttg catcgggcct ccctggccgc cggagaagag gccgctcgac tcgaacagga
     1381 agccaccgcg gcccgggagg ccaaggcctt ccaggaccga ttgatgcgct attactacct
     1441 cgacgaccag gagatggcgt tggtgctgaa cgaggaggat cgcagtcgct atttccaggc
     1501 ccgggcgaag gcgctggctc agcaggatga ggcagcccgg gcccatgagc aggctgccgc
     1561 atccaaggcc ctggccgata cgatccaggc gcaaagcagg gccatgctga cggcaaagga
     1621 taccggtgct gcggcagttg atgcggaggt catgcgtcgt gcggccctcc tttccgacca
     1681 ggctgtgcgc ctcaacctgc gatccgattc gctcgagcgc gtggcccgcc gcatggaact
     1741 ggcggccagc gccaacgccg cgcaggcggc caccatcctg caagggcttt cccctgagcg
     1801 tggcacggtg atcatggcca tggagcagcg cacacgacgt gtggaaccgg tactggcgga
     1861 ggcccgaagt ctccaggcag cggaaagcgt ccgggataca tcgggtggta cgcgtgacgg
     1921 tctggcacag gctcctgtcc gaacaggatc ccgttccagt gccacggata cagtactggt
     1981 atcaactacc aaagaacagg ttgctcagaa cgcgggtcaa gggaatgtgc cggcaccctc
     2041 cgccggtggg atcgcaacga ccccgaccag catgtccacg tcgcagccca gcactggatc
     2101 cgtggatgcg gcgatcactc cagcggctcc tgcactgcta cc
//
LOCUS       DAOQOC010000497         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33240_length_2139_cov_3.857965, whole genome shotgun sequence.
ACCESSION   DAOQOC010000497 DAOQOC010000000
VERSION     DAOQOC010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33240_length_2139_cov_3.857965"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PL106_08570"
     CDS             <1..197
                     /locus_tag="PL106_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33161.1"
                     /translation="LFSIRTENGVVILDGRGFGHGVGLCQEGAMHMARLGIPFTDILH
                     HYFAEVHLVDQGAIEFFKDE"
     gene            340..1731
                     /locus_tag="PL106_08575"
     CDS             340..1731
                     /locus_tag="PL106_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33162.1"
                     /translation="MIKTITLLNLLGILLFKAIFLTNVVVTHEAPATMQAGSEVKVTV
                     TVDKGPLSGFAKLQIDLPEGLTATAIETKGASFTFADGKAKFIWMALPSQPQFKVTYT
                     LGATATATGSLPITGKFSYIEDNERKTEDINSTTVTITGGAVAHDASPAPINSTITAT
                     NANDLTSAGGGAPISTEAPVAEPSVPPQQGVGGVLGARIITPVTEREMLVEVTIQKAD
                     LRGFGKLTETIPAGFTALEKNSAEAIFTTQDRIVKFVWLNLPAAKELKVIYKLRANDQ
                     ADGNYTINGDFGYLLNDETQRAVIGSTEFAVGPKAFAQQAVATTEPTKQDDATQTTAK
                     GEPSKPTKQTDVVARNDRPKASKPTATKVSGPETGVIYKVQIMAAHREVGSAYFNSQF
                     RYSGDFSIEHHEGWIKYVTGRYGQYREARDQRQNFIAAGHNFRGPFVTAYNNGDRITV
                     QEALMLSNQKWAQ"
     gene            1864..>2139
                     /locus_tag="PL106_08580"
     CDS             1864..>2139
                     /locus_tag="PL106_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33163.1"
                     /translation="MHDTVRTSVLCGLLSFAALQASAQSDSVVHLLRPTFALGSGAFA
                     FYGDIGAHHKGYSPLVTRLGFELRASTAITPWLEGGLFAQHGQLGANE"
BASE COUNT          454 a          732 c          597 g          356 t
ORIGIN      
        1 ccctcttcag tatccgcacc gagaacggcg tggtgatcct cgatggtcgc ggtttcgggc
       61 acggcgtagg cctctgccag gaaggagcga tgcacatggc ccggctcggc atcccgttca
      121 ccgatatcct gcatcattac ttcgccgagg tgcatctggt ggaccagggg gcgatcgagt
      181 ttttcaagga cgaatagccc cgcccccccc gcgtcccgac acccaggtat caatcctctg
      241 atcttcacac gcgcctgttg gaagatccgc attggcgcgg tccgcatcgc gcaatgaccc
      301 cgggtagttt cggcccggtt cataccgaaa acgcggacca tgatcaaaac catcacgctg
      361 ctcaatctcc tgggcatcct gctgttcaag gccatcttcc tcaccaatgt ggtggtcacg
      421 cacgaggccc ccgccaccat gcaggccggc tccgaagtga aggtgaccgt aacggtggac
      481 aagggaccgc tgagcggctt cgccaaactg cagatcgacc tgcccgaggg actcaccgcc
      541 accgccatcg aaaccaaggg cgcctcgttc accttcgccg atggcaaggc caagttcatc
      601 tggatggcgc tgcccagcca gccccagttc aaggtgacct ataccctcgg tgccacggca
      661 acggcgaccg gcagcctgcc gatcaccggc aagttctcct acatcgagga caatgagcgc
      721 aagacggagg acatcaactc caccacggtg accattaccg gaggagccgt ggcgcatgac
      781 gcgagccccg cacccatcaa ttccacgatc accgccacca atgccaacga cctcacttcc
      841 gccggtggtg gcgcaccgat atccaccgaa gcccccgtcg cggagccgag cgtccctccc
      901 cagcagggtg taggcggtgt tctcggtgcg cgcatcatca ccccggtcac agagcgtgag
      961 atgctcgtgg aggtgacgat ccagaaagcg gatctgcgcg gcttcgggaa actcacggaa
     1021 acgatccccg ccggttttac cgcgctggag aagaacagcg ccgaagccat cttcaccacg
     1081 caagaccgga tcgtgaagtt cgtgtggctc aacctgcccg cagccaagga attgaaggtg
     1141 atctacaaac tccgcgcgaa cgatcaggcc gacggcaact acaccatcaa cggcgacttc
     1201 ggctacctgt tgaacgacga gacccagcgc gccgtgatcg gcagtaccga gttcgcggtc
     1261 ggacccaaag cattcgctca gcaagccgtg gccaccaccg agcccaccaa gcaggatgac
     1321 gctacgcaaa ccacggccaa aggagaacct tccaagccca ccaagcagac cgacgttgtc
     1381 gcgaggaacg acaggcccaa ggcctccaag cccaccgcga ccaaggtgtc cggtcccgag
     1441 accggtgtga tctacaaggt gcagatcatg gccgcacacc gcgaggtggg cagcgcgtac
     1501 ttcaacagcc agttcaggta cagcggcgat ttcagcatcg aacaccacga gggatggatc
     1561 aaatacgtca ccggccgtta cggtcagtac cgcgaggccc gcgaccagcg tcagaacttc
     1621 atcgccgcag ggcacaactt cagggggcct tttgtcaccg cctacaacaa cggggatcgc
     1681 atcaccgtgc aggaggccct catgctgagc aaccagaagt gggcgcaatg accatttcat
     1741 gggaccggac aatggccacg cccgtaagcg tggccattgt ttttccggcc ggcggcaact
     1801 cccccgttca tgttccggag ccgcggctat ctttcgcggg cttcccttga cgctgaacga
     1861 cccatgcacg acaccgtacg aacttccgtt ctctgtggcc tgctatcctt cgcagcgctc
     1921 caggcatcgg cccagagcga ctccgtggtg catctcttgc gccccacctt cgcattggga
     1981 tccggtgcat tcgcctttta cggcgatatc ggcgcgcacc acaaaggcta cagtccattg
     2041 gtcacccgtc tgggcttcga actgcgcgcg agcacggcga tcaccccttg gctcgaaggg
     2101 ggcctcttcg cacagcacgg ccaactcggc gccaacgag
//
LOCUS       DAOQOC010000498         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33391_length_2134_cov_2.643098, whole genome shotgun sequence.
ACCESSION   DAOQOC010000498 DAOQOC010000000
VERSION     DAOQOC010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33391_length_2134_cov_2.643098"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="PL106_08585"
     CDS             complement(<1..2042)
                     /locus_tag="PL106_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA33164.1"
                     /translation="MNALTPNQDAPNPLFGINGCALQYFRFQDLLKQDTPIHLNGHPN
                     PCDNNVQVPTSIPHFHHTRPIIDWKHGQNISRSMGFNGNTAVTYQLDAQGAPVPGPAF
                     AGNASVGGCFISGLGWPAGYQNTYMHGDYGGAWIKRFTMNAQDQPVSVANFAGGAGAV
                     VFVKEGLDGALWYVRFEYGAIWKISPVGITNLPPVAVATQSVQYGPGPLSVNFNASGS
                     TDPENGPLTYAWNFGDGGQATGVTTNHTFTPGNSQPTSYTVTLTVTDNGGLTNQTTLL
                     VSVNNTPPVVAITSFPDGQLYPPGVDTTVALTADVTDLEHGPGQLAYQWQTIFHHNNH
                     THPEIPVADPVSSTVIGGEGCYTDVFNYEVKLTVTDAGGLSTTVIHWLYPRCSTIAPT
                     AIIYSDVSIGEGPLSVQFNGAASTDNGTIVNYTWDFSDGTGATGAVTSKTFTDPGTYY
                     VTLVVTDDVGLVGTATKVITVIDHLPPQCVGATGSIQRQVYTGISGTAIANLLAAPNY
                     PASPSTTNYPTSFQSPVDVANDYGTRMRGYIIAPTTGNYTFIGVGDDECQTYLSLNAD
                     PQFKQLICSVPGWTNTGQLDKYPQQTSAAISLVAGRYYYVEVLHKEGGGGDHVSLWWR
                     TPGNSTAVVIPGTALAPWQDCPPSVVVRTNLQGAFVTSNNLMRDNLRTASLIPSTEP"
BASE COUNT          377 a          624 c          672 g          461 t
ORIGIN      
        1 ggttcagttg atgggatcag cgatgccgta cgcaggttgt cgcgcatcag gttattggat
       61 gtgacgaaag ccccttgcag gttggtgcgg accaccacgc tcggcgggca gtcctgccag
      121 ggggccaggg ccgtgcccgg tatgacgacc gcagtgctgt taccgggcgt tcgccaccac
      181 aacgacacat ggtcgcctcc acccccttcc ttgtgcagca cctccacgta atagtaacgg
      241 cccgctacaa gcgatatcgc tgcactcgtt tgctgggggt acttgtccaa ctggccggta
      301 ttcgtccagc cgggtaccga gcagatcaat tgcttgaact gcggatccgc attcaggctc
      361 aagtaggttt ggcactcatc gtctcctacc ccgatgaacg tataattgcc ggtggtcggg
      421 gcgatgatgt acccgcgcat ccgtgttccg tagtcattgg ccacgtcaac cggggactgg
      481 aacgaggtgg gatagttggt cgtgctgggc gacgctggat aattgggggc ggccaggagg
      541 ttcgcgatcg ccgttccgct gatacccgtg tacacctgtc gctggatgct gcccgtggcg
      601 cccacgcact gcggggggag atggtcgatc accgtgatca ccttggtggc cgtgcccacc
      661 agtcctacgt catcggtgac caccagtgtg acataatagg tccccgggtc agtgaatgtt
      721 tttgacgtga ccgcaccggt ggcccccgtt ccatcggaga aatcccacgt ataattcacg
      781 atggtcccgt tgtcggtact ggcagcgcca ttgaattgta cgctgagtgg cccttcgccg
      841 atggaaacat ccgagtagat gatcgccgtg ggtgcgatgg tgctgcaccg cggatagagc
      901 cagtggatca ccgtggtgct caggcctccc gcatccgtaa ccgtgagttt cacctcgtaa
      961 ttgaacacgt cggtgtagca gccctcgcct ccgatcacgg tgctgctgac ggggtccgct
     1021 accgggatct ccgggtgggt gtggttgttg tggtggaaga tcgtctgcca ttgataggcc
     1081 agttgtccgg gtccatgttc cagatccgtg acatcagcgg tgagcgcaac ggtcgtatcc
     1141 acgcccggcg ggtacaattg cccatcgggg aaactcgtga tggccaccac gggtggcgtg
     1201 ttgttcacgc tcacgagcaa ggtggtctgg ttggtgaggc cgccattgtc cgtcaccgta
     1261 agcgtgaccg tgtagctggt cggctggctg ttgcccgggg tgaacgtgtg gttcgtggtc
     1321 actcctgtgg cctgccctcc gtcaccgaag ttccaagcgt aggtgagcgg cccattctcg
     1381 gggtccgtac tgcccgatgc gttgaaattg acactgagcg gccctggacc gtactgtacg
     1441 gattgtgtgg ccaccgccac cgggggcagg ttggtgatcc ccaccgggga gatcttccag
     1501 atggcgccgt actcgaagcg cacataccag agcgcaccat caagtccttc cttcacgaag
     1561 acgaccgcac cggctccacc ggcgaaattg gccaccgaca cgggttgatc ctgtgcgttc
     1621 atggtgaacc gcttgatcca agcaccaccg tagtcgccgt gcatataggt attctggtag
     1681 ccggcgggcc agcccagacc ggaaatgaag catccgccta ccgaggcatt gcccgcgaaa
     1741 gcaggcccgg gaacgggtgc accttgcgcg tccaactggt aggtgacggc cgtgttcccg
     1801 ttgaatccca tgctacggct gatgttctgg ccgtgtttcc aatcgatgat cgggcgtgtg
     1861 tggtggaagt gcgggatgct cgtgggcacc tgaacgttgt tgtcacacgg gttcggatgc
     1921 ccgttgaggt gtatgggcgt gtcctgcttg agcaggtcct ggaaccggaa gtactgcagc
     1981 gcgcatccat tgataccgaa caaggggttc ggtgcgtcct ggttcggggt cagtgcattc
     2041 atatacgagg cgttcgcgtc cccaccctca aaaagcggcc aaccgaaatt ctgccccccc
     2101 tcggtgcaaa cgttcagatc ctcccaggtg cccc
//
LOCUS       DAOQOC010000499         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33392_length_2134_cov_2.636845, whole genome shotgun sequence.
ACCESSION   DAOQOC010000499 DAOQOC010000000
VERSION     DAOQOC010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33392_length_2134_cov_2.636845"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..418)
                     /locus_tag="PL106_08590"
     CDS             complement(11..418)
                     /locus_tag="PL106_08590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA33165.1"
                     /translation="MFERACRLRVRYAETDRMGYVYYGVYAQYFEVARVEALRALGIS
                     YREMEDGGVMLPVHDLSLKYHKPALYDDELTIIARISTLPTVRIRFTYEVRNGRDVLL
                     TEGETTLVFVDKVTGRPCRAPQDLLDRMSPYFS"
     gene            781..>2134
                     /gene="dnaA"
                     /locus_tag="PL106_08595"
     CDS             781..>2134
                     /gene="dnaA"
                     /locus_tag="PL106_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNA33166.1"
                     /translation="MKKDHEKIWTRCLEVIQDNVSQQSFKTWFEPIRPLKLADAVLTI
                     QVPSQFFYEWLEEHYIGLLKKTIKKELGTEGRLEYSIIMENAAKNAHPYSVRMPTSNS
                     SAVKNPAVNLPIDVANSPIKNPFIIPGLKKIKVESHLNPNYSFENFIEGDCNRLARSA
                     GFAVANKPGGTAFNPLLLYGGVGLGKTHLAHAIGIEIKKNHPEKTILYVPAEKFTQQF
                     IESVRNNTSGDFTQFYQMMDVLIIDDVQFLAGKEKTQDVFFHIFNHLHQAGKQIVLTS
                     DKAPVEMQGVEQRLLSRFKWGLSADLQQPALETRIAILHKKMYAEGIELPKEVVEYLA
                     YSITTNIRELEGAMVSLIAQSSLNKKAVTLELAKQMIDKFVKNTAREVSIDYIQKVVC
                     DYFDLPIELLKSKTRKREVVQARQIAMYFAKKMTKNSLASIGAHCGGKDHATVLHACR
                     T"
BASE COUNT          499 a          673 c          581 g          381 t
ORIGIN      
        1 cttggaacga tcaagaaaaa tagggagaca tccggtccaa caggtcctgc ggcgcacggc
       61 atggccgacc cgtgaccttg tcgacgaaca ccagagtggt ctccccttcg gtgaggagga
      121 catcccgtcc gttgcgcacc tcgtaggtga accggatcct caccgtgggc aacgtcgaga
      181 tgcgtgcgat gatggtgagt tcgtcatcgt acagcgcggg cttgtggtac ttcagcgaaa
      241 gatcatgcac cggcagcatc acgccgccat cctccatttc ccggtacgaa atacccagcg
      301 cgcgcaacgc ctccacgcgg gccacctcga aatactgcgc gtacacaccg tagtacacgt
      361 agcccatacg gtccgtctcg gcatagcgga cacgcaaccg gcacgcacgt tcgaacatgc
      421 cggcaagata ggtccggcgg gcatccctgc ctgtttgcgt accgatcaca acatccgtac
      481 tttcacggtc cgttccgccg ggcttccggt catcatgccg cacaccttct tggcaccacc
      541 tgtatcgcga tcacggcgtc gcggcatgac ggaacaacgg accgtgtcgc gccgggataa
      601 tagcacttcg tccacgtctt tgggcgcatc gaaaatattt ccgctccatc aagtggtcct
      661 caactttttt tttcaccccg gcgggtccat atttgcgacc ccttcagcgg agcccccttt
      721 ccgaatgaag gatcgtcatc gcactatccc gctcgtcatc cctaacccac cgacccgccc
      781 atgaagaaag accacgagaa gatctggacg cggtgcctcg aggtgataca ggacaacgtg
      841 agccagcaga gcttcaagac ctggttcgaa ccgatcaggc ccttgaaact ggctgacgcc
      901 gtcctgacca tacaggtgcc atcccagttc ttctatgaat ggctggagga gcattacatc
      961 ggacttctca agaagacgat caagaaagaa ctgggcaccg aaggccgcct cgagtactcc
     1021 atcatcatgg agaacgcggc gaagaacgcg cacccatact cggtgcgcat gccaacgagc
     1081 aattccagcg cggtgaagaa cccggctgtg aacctgccca tcgatgtggc gaacagcccg
     1141 atcaagaacc cgttcatcat ccccgggctc aagaagatca aggttgaaag ccacctcaac
     1201 ccgaactact cgttcgagaa cttcatcgag ggcgattgca acaggctggc gcgcagtgcc
     1261 ggtttcgccg tggcgaacaa gcccggtggc accgccttca atcccttgct gctctacggc
     1321 ggtgtgggac tgggcaagac ccacctcgcg cacgcgatcg gtatcgagat caagaagaac
     1381 catccggaaa agaccatcct ctacgtaccg gccgagaagt tcacgcaaca gttcatcgag
     1441 agcgtgcgca acaacaccag tggtgacttc acgcagttct atcagatgat ggacgtgctc
     1501 atcatcgatg atgtgcagtt cctcgcgggc aaggagaaga cccaggatgt gttcttccac
     1561 attttcaacc acctgcacca ggccggcaag cagatcgtgc tcaccagcga caaggcgccc
     1621 gtggagatgc agggtgtgga acagcgtctc ctcagccgct tcaagtgggg cctcagcgcc
     1681 gaccttcaac agcccgcact cgaaacgcgc atcgccatcc tgcacaagaa gatgtacgcc
     1741 gaggggatcg agttgcccaa ggaggtcgtt gaatacctgg cctacagtat caccaccaac
     1801 atccgcgaac tcgagggcgc catggtcagc ctgatcgccc agagctcgtt gaacaagaaa
     1861 gcggtgacgc tggagctggc caagcagatg atcgacaagt tcgtgaagaa caccgcccgc
     1921 gaggtgtcga tcgactacat ccagaaggtg gtgtgcgatt acttcgacct gccgatcgag
     1981 ctgctcaaga gcaagacgcg caagcgcgaa gtggtgcagg cccgccagat cgccatgtac
     2041 ttcgccaaga agatgaccaa gaattccctg gccagcatcg gtgcccattg cggcgggaag
     2101 gaccacgcca cggtgctgca cgcctgccgc accg
//
LOCUS       DAOQOC010000500         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33474_length_2131_cov_3.119942, whole genome shotgun sequence.
ACCESSION   DAOQOC010000500 DAOQOC010000000
VERSION     DAOQOC010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33474_length_2131_cov_3.119942"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PL106_08600"
     CDS             complement(<1..626)
                     /locus_tag="PL106_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33167.1"
                     /translation="MSDPRPKRKKSFLARLKDKYRLSLVNESSFEEKLSFRLTKFNVL
                     LLAIVAFLVCAMLVTGFIVLTPVKRLIPGYSDLRTKRDAFHATMLADSLEGAMAVRDL
                     YLANLRSVLSGELPADSATLFRPITTTPGPEDLLPGSADSVMRERIRREEAYNVEQRT
                     GVEKEREGLSGIFFFAPLRGVVTGTFDKAQRHFGIDFVTKPDEAVKACL"
     gene            complement(703..>2131)
                     /locus_tag="PL106_08605"
     CDS             complement(703..>2131)
                     /locus_tag="PL106_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNA33168.1"
                     /translation="IVARFLNFLLVPLYTSNFVFEPAEYGVVTALYSWTALLNVVLTF
                     GMETTFFRFANKDHVKNNPDGGRVLYATATWGLFFPALAFMLLGGLFSQRIAEGLHFG
                     EYASSVLMLVGVIGIDAITAIPMARLRQEGRPWRYAFINLLSVGVNIGLSLFFLLYCM
                     KRYNMGERDALIDAVYDPSLGVGYVFVINLMSTAVKLLVLLPSWPSFNGFNRDLLRAM
                     FAFGAPLMVAGLAGMVNETADRVILKYLLPTDVADSELGIYGACYKLAVLITLFIQAF
                     RMGAEPFFFNHAGEKNSRQMFARIMNIFVAVCMTAFLTVMLFLDLFKWFIPNPAYHTG
                     LRVVPILMLANVFLGIYYNQSVWYKLTDRPRTGSTISLIGAAITIVLLLALIPTLGYM
                     GAAWATLICYASMAAISYAWGQRHYPIPYDVGRVLAYMAFGVFLWWACEHLPLTGMLK
                     YGFRMVVLAGAVALAWRMERNVIRT"
BASE COUNT          459 a          654 c          658 g          360 t
ORIGIN      
        1 aggcaggcct tcacggcctc gtcgggcttg gtcacgaaat cgataccgaa atgccgttgt
       61 gccttgtcga acgttccggt gacgacgcca cgaaggggtg cgaaaaagaa gatgccggag
      121 agtccttcgc gctccttctc cacgccggtg cgctgctcca cgttgtaggc ctcctcgcga
      181 cggatgcgct cgcgcatcac gctgtccgcc gatcccggca agaggtcctc gggaccgggc
      241 gtggtggtga tgggccggaa gagcgtggcg ctatcggcgg gcaactcgcc actgagcacg
      301 ctgcgcaggt tggccaggta caggtcccgc acggccatgg cgccctccaa tgaatccgcg
      361 agcatggtgg cgtggaatgc gtcacgcttg gtcctcaggt ccgaatagcc cgggatcaac
      421 cgcttcaccg gggtgagaac gatgaaaccg gtaacgagca tggcgcacac cagaaaagcc
      481 acgatggcca gcagcagcac attgaacttg gtgaggcgga aactcagctt ctcctcgaaa
      541 gacgactcgt tcaccagcga caaccggtac ttgtccttca gccgggcgag gaagctcttc
      601 ttgcgcttgg ggcgcgggtc ggacatgcgg attcgccgtg ggccggaacg gtggcgcggc
      661 agctaaggta ggaccggagc ggtgtcggtg ccgagatcct gttcaggtgc ggatgacgtt
      721 cctttccatt cgccatgcca gggccacggc tcctgcgagt accaccatcc tgaagccgta
      781 cttcaacatg cctgtcaagg gcagatgttc gcacgcccac cacaggaaca caccgaacgc
      841 catgtaggcc agcacgcgac ccacgtcgta cgggatcgga taatgccgct gcccccacgc
      901 ataactgatc gccgccatgc tggcgtagca gatgagcgtg gcccatgccg cccccatgta
      961 tcccagcgta gggatcaatg ccaagagcag cacgatggtg atggccgcac cgatcaaact
     1021 gatggtgctg cccgtgcgcg gccggtcggt gagcttgtac cacacgctct ggttgtaata
     1081 gatcccgagg aaaacgttgg ccagcatgag gatgggcact acgcgcaggc cggtgtgata
     1141 agccgggttg ggtatgaacc acttgaacag gtccaggaag agcatcactg tcaggaaggc
     1201 cgtcatgcac acggccacga agatgttcat gatgcgcgcg aacatctgcc ggctgttctt
     1261 ctcccccgcg tggttgaaga agaagggttc ggcgcccatc cggaaagcct gtatgaaaag
     1321 cgtgatcagc acggccagct tgtagcaggc accatagatg ccgagttcgc tgtcggccac
     1381 atcggtggga agcaggtact tcaggatcac gcggtccgcg gtctcgttca ccatgcccgc
     1441 gaggcccgcc accatcaagg gtgcaccgaa cgcgaacatc gcacgcagca gatccctgtt
     1501 gaacccgttg aaggatggcc atgaaggcag cagcaccagc aatttcacgg ccgtgctcat
     1561 cagattgatc acgaacacgt agcccacgcc gagcgatggg tcgtacaccg catcgatcag
     1621 cgcgtcccgc tcgcccatat tgtaccgctt catgcagtac agcaggaaga aaaggctgag
     1681 cccgatgttc acacccacac tgagcaggtt gatgaacgcg tacctccagg gtcgtccctc
     1741 ctgacgcaac cgggccatgg ggatcgccgt gatggcatcg atcccgatga cccccacgag
     1801 catcagcacc gaactcgcgt actccccgaa gtgcaatccc tcggcgatcc gctgggagaa
     1861 caagccgccc agcaacatga acgcgagcgc cgggaagaag aggccccagg tggcggtggc
     1921 atacagcacc cggccgccgt cggggttgtt cttcacatgg tccttgttgg cgaaccggaa
     1981 gaacgtggtc tccataccga aggtgagcac cacgttgagc agggccgtcc acgagtacag
     2041 cgcggtgacc accccgtatt cggccggctc gaagacgaag ttgctggtgt acagcggcac
     2101 cagcaggaaa ttgaggaagc gcgcaacaat a
//
LOCUS       DAOQOC010000501         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33507_length_2130_cov_2.623133, whole genome shotgun sequence.
ACCESSION   DAOQOC010000501 DAOQOC010000000
VERSION     DAOQOC010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33507_length_2130_cov_2.623133"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PL106_08610"
     CDS             <1..666
                     /locus_tag="PL106_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA33169.1"
                     /translation="ARAQKAGVDRPLVPEVRVGSLYDLIGTAAAEIGAGLIIMGTHGM
                     RGMQFITGSRALRVITNSAVPFIVVQERNIRPTGYQKIVVPLDLQKESRQKLTLVADM
                     AKYFKCKVYLVTPNEKDEFLHNQVVNHLRFANTYLNERGIEHEEHILEEGSDDIVQGT
                     IRYAVQVDADLITIVNTLQNSIFGVLGVPNEQEVITNAPQIPVMCMNPNTVGYTNAPV
                     FNV"
     gene            complement(733..>2130)
                     /locus_tag="PL106_08615"
     CDS             complement(733..>2130)
                     /locus_tag="PL106_08615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNA33170.1"
                     /translation="ERRVREILPELSSYKDPLVWIERNELAEEGGPLDQRTGLLLADV
                     DKLLSLQEAKRQNLSGGMIRSFDSLKFFVLYLGSGLVIVGIVVAILIVRSIVAPVQRL
                     RGVLLSLGRGVFPATRIHAHNDEVGDMSRALMGLIDGLKRTTEFSHAVAAGDFKAEYQ
                     PLSEEDVLGHALLKMRTELGERERVLETKVKERTEEVVRQKEEVERQGRKVVELYKNV
                     TDSIRYAKRLQESILPPDKRIKELLPESFVLYRPKDIVSGDFYWVEEVGDKVVFAAVD
                     CTGHGVPGAFMSLVGHNGLNQAVKERGNTRPSDVLHDLDRMSYEALHKDRDQFMRDGM
                     DMALCALDPKRMVLEYAGANCPLYLMRGDDIQQYAPDKRPIGGADPGDKRFTDHRIEL
                     RKGDLIYIFSDGYADQFGGPNGKKFLQRRFRELLISIRHETMERQKALLLEALNKWRG
                     AQEQVDDILVMGMRA"
BASE COUNT          401 a          702 c          614 g          413 t
ORIGIN      
        1 gcacgggccc agaaagccgg cgtggatcgg ccgctcgttc cggaggtgcg cgttggatcg
       61 ctctacgacc tgatcggtac ggccgccgcc gagatcggcg ccgggctcat catcatgggt
      121 acacatggta tgcggggcat gcaattcatc accggaagcc gtgcactgcg tgtgatcacc
      181 aacagtgccg tgcccttcat cgtggtacag gaacgcaaca tccggcctac cggttaccag
      241 aagatcgtgg tgcccctcga cctgcagaag gaaagccgcc agaaactcac gctggtggcc
      301 gacatggcca aatacttcaa gtgcaaggtc tacctggtca cccccaacga gaaggacgag
      361 ttcctgcaca atcaggtggt gaatcacctg cgcttcgcca acacctacct caacgaacgc
      421 gggatcgaac acgaggagca catcctggag gagggcagtg acgatatcgt gcagggcacg
      481 atccggtacg ccgttcaggt ggatgcggat ctgatcacca tcgtgaacac gttgcagaac
      541 agcatcttcg gtgtgctggg cgtacccaac gagcaggagg tgatcaccaa tgcgccccag
      601 atccccgtga tgtgcatgaa ccccaacacc gtggggtaca cgaacgcgcc cgtgttcaac
      661 gtgtgatagc ggagccgctg aggtcccggt cggccacgat cgcgtcctcg tccacctgtt
      721 cgaccccggg gctcacgccc gcatccccat cacgaggatg tcgtccacct gttcctgtgc
      781 accgcgccac ttgttcagcg cttcgagcag caatgccttc tggcgttcca tggtctcgtg
      841 ccggatggag atgagcagtt cccggaagcg gcgctgaagg aatttctttc cgttgggccc
      901 cccgaactgg tcggcgtagc cgtcgctgaa aatgtaaatg aggtcgccct tgcgcagttc
      961 gatgcggtga tcggtgaagc gcttgtcccc gggatccgct ccgccaatgg ggcgcttgtc
     1021 cggtgcgtac tgctggatat catcgccgcg catcaggtac aacgggcagt tggccccggc
     1081 gtattccagt accatgcgct tggggtccag ggcacagagg gccatgtcca tgccatcgcg
     1141 catgaactgg tcgcggtcct tgtgcaacgc ctcataggac atgcggtcca ggtcgtgcag
     1201 cacatcactg ggccgtgtgt tcccgcgctc cttcaccgcc tggttcaacc cgttgtgccc
     1261 tacaaggctc atgaaagccc ccggtacccc gtggccggtg caatccaccg cagcgaagac
     1321 caccttgtcc cccacttcct ccacccagta gaaatcgccg ctcacgatgt ccttcggacg
     1381 gtacaggacg aagctctccg gcaacaactc cttgatgcgc ttgtccggcg gaaggatgga
     1441 ttcctgcagg cgtttggcgt agcggatact gtccgtgacg ttcttgtaca gttcgaccac
     1501 cttccggccc tgacgctcca cctcttcctt ctgccgtacc acttcttcgg tacgctcctt
     1561 caccttggtc tccagcacgc gttcgcgttc gcccaactcg gtgcgcatct tcagcaaggc
     1621 gtgacccagc acgtcctcct cgctcagtgg ctggtactcc gctttgaaat cacccgccgc
     1681 caccgcgtgg ctgaactcgg tggtgcgctt cagcccatcg atgaggccca tgagcgctcg
     1741 tgacatgtcg cccacctcat cgttgtgcgc atggatgcgt gtggccggga agacaccccg
     1801 accgagggaa agcagcacac cgcgcaggcg ttgcacgggc gccacgatgc tgcgcacgat
     1861 caggatggcc accacgatgc ccacgatcac cagaccactc ccgaggtaga gcacgaagaa
     1921 cttcaaggag tcgaagctgc ggatcatgcc accactgagg ttctggcgct tggcctcctg
     1981 cagcgagagg agcttgtcca catcggccaa caacaggccg gtccgttggt ccagcggtcc
     2041 cccctcctcg gccagttcat tgcgttcgat ccacaccagg gggtccttgt aactgctgag
     2101 ttcgggcagt atctcccgga cacgccgctc
//
LOCUS       DAOQOC010000502         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33519_length_2129_cov_4.422854, whole genome shotgun sequence.
ACCESSION   DAOQOC010000502 DAOQOC010000000
VERSION     DAOQOC010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33519_length_2129_cov_4.422854"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PL106_08620"
     CDS             <1..886
                     /locus_tag="PL106_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HNA33171.1"
                     /translation="NEGFTVYFEHRITEELYGPEDNAMTSLIGHQDLMNTVADMEAKG
                     DPGDTRLKLDLAGRDPDDGMTDVAYEKGFALLRLVESRVGREKFDAFLRGYFDRYAFQ
                     SMTTERFITYLHEELLDPANVTVDVERWVYQPGIPEDDIVPVSDRFTKVEEQIARWGN
                     GTPAKELATQGWSTFEWIHFLRHLPKGLEEGSMADLDRSFGFTRSGNTEILAAWLEQC
                     ILNDYDAAYLRLDEFLRTVGRRKFLIPLYQELVATEKGRTIAEGIYAAARPNYHSVSV
                     RTIDAMLGWEGRQGSITL"
     gene            complement(876..1514)
                     /locus_tag="PL106_08625"
     CDS             complement(876..1514)
                     /locus_tag="PL106_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33172.1"
                     /translation="MEEGSIRVPRTARYHLLGEADTARALWVVLHGYGQLARYFLRNF
                     EGLKNDLLIVAPEGLSRFYTDIGHTRVGATWMTREDRTHEIADQISYLDLLADTVLRS
                     CPPGTPLHVLGFSQGVATACRWLGLGRAHAQRLVLWGGSLPPDLPVNAWTVQLVTTHV
                     ELVHGIKDTLVPGSVVAASEALLRDAGIPHETHRFDGGHELDPFLLKRLITA"
     gene            complement(1520..>2129)
                     /locus_tag="PL106_08630"
     CDS             complement(1520..>2129)
                     /locus_tag="PL106_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNA33173.1"
                     /translation="CNIKGIKVAYPSTGADLKGLMKAAYHDPNPVVMLEHKGLYWSKI
                     KGTEDAKSIEPSPDYIVPFGKARMVQQADEVRISRGEAAVIVTYGMGVYWARAAARAF
                     PGRVSIVDLRTLVPMDEDAVMDAVRAHGRCLVVTEEQLTNSFAQALAGRIGDVCFRDL
                     DAPVRTLGAIDMPAVPLNSTLEAAMIPNAEKVEVVLGELLDY"
BASE COUNT          408 a          646 c          683 g          392 t
ORIGIN      
        1 gaacgaaggc ttcaccgtgt atttcgagca caggatcacc gaggaattgt acgggccgga
       61 ggacaacgcc atgacgagcc tgatcggcca ccaggacctg atgaacaccg tggcggacat
      121 ggaagccaag ggagatcccg gcgataccag gctgaagctc gacctggccg gtcgcgatcc
      181 ggatgatggc atgacggatg tggcgtacga gaagggtttc gcgttgctcc gtcttgtgga
      241 aagccgggtg ggtcgcgaga agttcgatgc gttcttgcgc ggctacttcg atcgctacgc
      301 attccagagc atgaccacgg aacgcttcat cacgtacctg cacgaggaac tcctggatcc
      361 cgccaatgtg accgtggatg tggagcgctg ggtatatcag ccgggcatcc ccgaggacga
      421 tatcgttccg gtgtcggacc gcttcaccaa agtggaggag cagatcgcgc gctggggaaa
      481 tggtactccg gcaaaggaac tggctacgca ggggtggagc accttcgagt ggatacactt
      541 cctgcgccat ctgcccaagg gattggagga gggcagcatg gccgacctgg accggtcatt
      601 cggtttcacg cgcagcggca acaccgagat cctggcggcc tggttggaac agtgcatcct
      661 gaacgactac gatgcggcgt acctccggtt ggacgagttc ctgcggaccg tggggcggcg
      721 caagttcctg atcccgctgt accaggagtt ggtggccacg gagaagggcc gaacgatcgc
      781 cgagggcatc tacgccgcgg cacggcccaa ctatcacagc gtgagcgtgc gtacgatcga
      841 cgcgatgctc ggctgggaag gtcgccaggg gtcgatcacg ctgtgatcag gcgcttcagc
      901 aggaagggat ccagttcatg gccaccgtcg aaacgatggg tctcgtgcgg tatacccgca
      961 tcacgcagga gcgcctcact cgcggcgacc acggatcccg gcacaagggt atccttgatg
     1021 ccatgaacaa gttcgacgtg tgtggtcacc agttgcacgg tccaggcgtt cacgggaagg
     1081 tccggaggca ggctgccgcc ccagagcaca agccgttgcg catgagcccg ccccaggccg
     1141 agccaacggc acgcggtggc caccccttgt gagaatccca gcacgtgcag tggtgtgccc
     1201 ggcgggcagc ttctcaacac ggtgtccgcg agcaggtcga ggtagctgat ctggtcggcg
     1261 atctcgtgcg tgcggtcctc acgggtcatc cacgtggcac ccacgcgggt gtggccgatg
     1321 tcggtgtaga accggctcag tccttctggc gctacgatca acaggtcgtt cttcagtccc
     1381 tcgaagttcc tcaggaagta acgtgcgagt tggccgtaac catgcagtac cacccacaag
     1441 gcacgagcgg tatcggcttc gcccagcagg tgatagcgtg cggtgcgggg tacgcgtatc
     1501 gaaccctctt ccatccggat cagtagtcca gcaattcacc cagcaccact tccaccttct
     1561 cggcattggg gatcatggcc gcttccaacg tgctgttcaa cggaactgcg ggcatgtcga
     1621 tcgcgccaag ggtacgcacg ggagcgtcca ggtcacggaa gcagacgtct ccgatacgcc
     1681 cggcgagtgc ttgcgcgaag gagttggtca actgttcctc ggtgaccacg aggcagcggc
     1741 cgtgcgcacg cacggcatcc atcacggcat cctcatccat cggtaccaat gtgcgcaggt
     1801 ccacgatgct cacacgaccg gggaaggccc gtgcggcggc tctggcccaa taaacgccca
     1861 taccgtacgt gacgatcacg gcggcctcgc cacggctgat gcgcacctca tcggcctgct
     1921 gcaccatccg ggccttgccg aagggcacga tgtagtccgg tgatggttcg atgcttttgg
     1981 catcctcggt ccccttgatc ttgctccaat acaggccctt gtgttccagc atcaccacgg
     2041 ggttcgggtc gtggtaggcc gccttcatca ggcccttcag gtcggcaccg gtgctcgggt
     2101 aggccacctt gatgcccttg atgttgcag
//
LOCUS       DAOQOC010000503         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33568_length_2128_cov_3.031838, whole genome shotgun sequence.
ACCESSION   DAOQOC010000503 DAOQOC010000000
VERSION     DAOQOC010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33568_length_2128_cov_3.031838"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PL106_08635"
     CDS             complement(<1..665)
                     /locus_tag="PL106_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33174.1"
                     /translation="MRIQPAPALTALLLLCVITSPVRGQVSFGGTPITILSPGKLPHV
                     LWTDLPAVDVAPLLAEDAQRLASGDKKMRFGVTRPVDIGLSSAGSWHELPDGRRVWRV
                     GIHCPDASSVSFVFDDYRVVEGGQVFVIGSDDLFIGAFEQASAGGATSMAVMPVDGDR
                     VIVEYIVPAGHRPGALHIGRVTHGYKPLNDTDRGFGASGSCNNNVVCPEWNAWDDQIR
                     SVA"
     gene            complement(727..>2128)
                     /locus_tag="PL106_08640"
     CDS             complement(727..>2128)
                     /locus_tag="PL106_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33175.1"
                     /translation="CTGACNGPVKTGDVNENGAQEISDAQMYVNMIIGNDATATPCTD
                     LNDDGVITVTDAALMANCNNRQANHDQQPHLVHYHPWCSFPRGYLSIPDTVDLTIGAF
                     DPNGQYVDIWVKNSACRTMGFEFTMSGLTIQSVTNLDPQVQGDLLWAGALGGTKVVGI
                     CYNDSSLAKHTGFSPLCRINYFELTGAQICIASIQDIVNNGANNVVARIVDGCLNTGN
                     AVAADIKVLLEGPYQGAGMMDDALRTASLVPGAEPYTTLGFTQTGGGGEVLGAGVLDV
                     VGNNAIVDWVLVELRNAQSPAQVVATRCGLLQRDGDIVSVDGMGSLILPAVPGSYHVA
                     VRHRNHFGVMTASPVVLSGSSTAIDFTQTGTATWGTDARKNFGGGLWGLWSGNTRRDG
                     NISLLKYTGGNNDRDPILVIVGSSTPTAIVAGYSSEDANLNGVVKYTGNGNDRDPILV
                     NVGSILPNGIRTEQLP"
BASE COUNT          382 a          688 c          654 g          404 t
ORIGIN      
        1 ttggccacgg agcggatctg atcgtcccat gcgttccact ccggacatac cacgttgttg
       61 ttgcaactgc cgctcgcacc gaatccccgg tcggtatcat tcaaaggctt gtatccatgg
      121 gtcacacggc cgatgtgcaa tgcgcccggt cgatgaccgg cgggcacgat gtattccacg
      181 atgacgcggt ccccgtccac cggcatcacg gccatggacg tcgcacctcc ggcacttgcc
      241 tgctcgaagg ctccgatgaa cagatcatcc gatccgatca cgaacacttg accaccttcc
      301 acaacccggt agtcgtcgaa cacgaaactc acactggacg cgtccggaca atggatgccc
      361 acccgccaga cgcgacgtcc atcggggagt tcgtgccagc ttccagcgct cgacaggccg
      421 atatcgaccg ggcgggtcac gccgaatcgc atcttcttat ccccggaggc caggcgttgc
      481 gcgtcctcgg caagaagcgg tgcgacatcc accgcaggca ggtccgtcca cagcacgtgc
      541 gggagcttgc ccggtgagag gatcgtgatc ggggtgcccc cgaaggagac ctgaccacgc
      601 accgggctgg tgatcacgca caggaggaga agggccgtca gggccggagc aggttggatc
      661 cgcatggtag aacaaggtcg gagcagggcc gaaactaggg tcgttgcgct ccgatgtcca
      721 gcggactcac ggcagttgct ccgttcgtat cccgttcggc aggatgctgc cgacgttcac
      781 caggatgggg tcccgatcgt tgccgttgcc ggtgtacttc accacgccgt tcaagttcgc
      841 gtcctccgac gaataaccgg ccactatcgc cgtgggcgtg gaactgccca cgatcaccag
      901 gatgggatcg cgatcgttgt tgccaccggt gtacttcagc aggctgatgt tgccgtcgcg
      961 acgggtgttg ccgctccaga gcccccacaa gccaccgccg aagttcttgc gcgcgtcggt
     1021 tccccaggtc gccgttccgg tctgggtgaa gtcgatggct gttgaactgc cactgagcac
     1081 gaccggcgaa gccgtcatca ccccgaaatg attccgatgc cgcaccgcta cgtggtaact
     1141 gccagggacc gcgggcagga tcagcgaacc cataccgtcc acggatacga tgtccccgtc
     1201 gcgctggagc aggccgcagc gtgtggccac cacttgcgcc ggggactgcg cgttgcgcaa
     1261 ctccaccagc acccaatcca cgatggcatt gttgccgacg acatcgagca cgcccgcacc
     1321 gagcacctct cctcctccgc cggtctgcgt gaaaccgagc gtcgtgtacg gctccgcccc
     1381 ggggacaagg gatgccgtcc tgagcgcatc atccatcata cccgcaccct ggtatggccc
     1441 ttcgagcagc accttgatgt cggccgccac ggcgttgccc gtgttgagac atccatcaac
     1501 gatgcgcgcc accacgttgt tcgccccgtt gttcacgatg tcctgaatgc tcgcgatgca
     1561 gatctgcgca cccgtgagtt cgaagtagtt gatgcggcag agcggtgaga agcccgtgtg
     1621 tttggccagg ctgctgtcgt tgtaacagat cccgaccact ttggtcccgc ccagggcacc
     1681 ggcccacagc agatcaccct gcacctgggg gtcgaggttg gtcacggact ggatcgtgag
     1741 accgctcatg gtgaattcga agcccatggt gcggcacgca ctgttcttca cccagatgtc
     1801 gacgtactgg ccgttgggat cgaacgctcc gatggtgagg tccaccgtgt cgggaatgct
     1861 gaggtaacca cgcggaaagc tgcaccacgg atggtagtgg accaggtggg gttgctggtc
     1921 gtggttggcc tgccggttgt tgcagttggc catcagggcg gcatcggtca cggtgatgac
     1981 accatcatca ttcaggtcgg tgcagggcgt ggcggtggca tcgttgccga tgatcatgtt
     2041 cacgtacatc tgcgcgtccg agatctcctg ggccccgttc tcgttcacgt cgccggtctt
     2101 caccgggccg ttgcacgcgc cggtacag
//
LOCUS       DAOQOC010000504         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33606_length_2127_cov_2.405405, whole genome shotgun sequence.
ACCESSION   DAOQOC010000504 DAOQOC010000000
VERSION     DAOQOC010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33606_length_2127_cov_2.405405"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PL106_08645"
     CDS             complement(<1..1266)
                     /locus_tag="PL106_08645"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNA33176.1"
                     /translation="MKHRTIIEPFRIKSVEPIGLSTEAQRAEQLKAAHYNPFLLRSED
                     VVIDLMTDSGTGAMSAHQWAGVMEGDEAYAGSRSWVRMEKAVQDLTGMRHVLPTHQGR
                     AAERIIYGHLGGAGKVFISNTHFDTTRANIEYSGATAIDIPIPEGQDPAAQHPFKGNM
                     DLEQLDQLLKQHQGRVGAVILTVTNNSGGGQPVSLANAEGVSAICKRHGALFMLDCCR
                     VAENSWFVKHREAGMGNRSYREIAQRMFALADGAVMSAKKDGMVNMGGFLALRDADLA
                     EACTNLLIITEGFTTYGGLSGRDMEAIATGLDEVFDPHYLDYRIRSTTFLGDRLHELG
                     VPLMRPIGGHAVYIDAKALYPHIPPAQYPGQALVCELYLLGGIRSVEIGSVMFGTYGE
                     DGSLRPARMELGRLAIPRRVYTQSHIEYVA"
     gene            complement(1309..>2127)
                     /locus_tag="PL106_08650"
     CDS             complement(1309..>2127)
                     /locus_tag="PL106_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33177.1"
                     /translation="GTGWGSPFLFVPEATNVDPATLEQLIQARPEDYFLSQASPLGVP
                     FHNFRHSSSEQQRTTRIAKGRPGSPCFKEFLSNNTEFTERPICTASRQYQHLKLARLR
                     STITDPKVLAREEAAVTAKDCLCEGLGAGALLKAGMKPAHKLEAVAICPGPNAAYFHK
                     RISLQRMVDHIHGRLDLLAGVDRPHMFVKELSLYVDYFRGEIERFASEINAKQQRRLV
                     AFRGNLLEGVQHYIDLLPVLQERLAADAQRMRADLTRYADEIRSLVFPEPLPQT"
BASE COUNT          336 a          643 c          721 g          427 t
ORIGIN      
        1 cgccacgtat tcgatgtggc tctgggtgta cacccggcgc gggatggcca gtcgtcccag
       61 ttccatgcgg gcgggccgaa ggctgccgtc ctcgccataa gtgccgaaca tcaccgagcc
      121 gatctccacg cttctgatac cacccaatag gtatagttcg cagaccaggg cctggccggg
      181 gtattgcgcg ggcgggatgt gcgggtagag cgccttggca tcgatgtaca cggcatggcc
      241 accgatcggg cgcatcaagg gtacgcccag ttcatgcagc ctgtccccga ggaaggtggt
      301 actgcggatc cggtagtcca gatagtgcgg gtcaaagacc tcatcaagcc cggtggcgat
      361 ggcttccatg tcgcgcccgc tcaggccgcc gtacgtggtg aaaccctcgg tgatgatgag
      421 caggttggtg caggcctcgg ccaggtccgc atcgcgcagg gccaggaagc cgcccatgtt
      481 caccatgccg tccttcttgg cgctcatcac ggcaccgtcg gccagggcga acatgcgctg
      541 ggcgatctcc cggtaggagc ggttccccat gccggcttcg cggtgcttca cgaaccagct
      601 gttctcggcc acacggcagc agtccagcat gaagagcgca ccatgccgct tgcagatggc
      661 ggatacgccc tcggcattgg ccaagctcac gggctggccg ccaccgctgt tgttggtcac
      721 cgtgaggatg acggcgccca cccggccctg gtgctgcttc agcagttggt ccagttgctc
      781 caggtccatg ttgcccttga aggggtgctg tgcggcggga tcctgtcctt cgggtatggg
      841 aatgtcgatg gcggtggccc cggagtactc gatgttggcc cgggtggtgt cgaagtgcgt
      901 gttgctgatg aacaccttgc ccgcgccgcc gaggtggccg taaatgatgc gttctgccgc
      961 tcgaccctgg tgggtgggca gcacgtgccg catgccggtg aggtcctgga cggctttttc
     1021 catgcgcacc catgagcgcg agcccgcata ggcctcatcg ccttccatca ccccggccca
     1081 ctggtgggcg ctcatggccc cggtgccgct atcggtcatc aggtcgatca ccacgtcctc
     1141 ggagcgcagc aggaaggggt tgtagtgcgc ggctttgagc tgttcggcac gttgtgcctc
     1201 ggtactcagg ccgatgggct ccacgctctt gatgcggaac ggctcgatga tggtacggtg
     1261 cttcatggtg cggtgggcgc ttccggtcgt gtgaatggtg cgatccggtc aggtctgggg
     1321 cagtggttca gggaatacca gggagcggat ctcatcggca taccgggtca gatcggcccg
     1381 catgcgttgc gcatcggccg ccagccgctc ctgtaatacc ggtaacaggt cgatgtagtg
     1441 ctgcacccct tccagcaggt tgccccggaa ggcgacaagg cggcgctgtt gcttggcgtt
     1501 gatctccgat gcgaagcgtt cgatctcgcc gcgaaagtag tccacataga gtgagagttc
     1561 cttgacgaac atgtgcgggc ggtccacacc cgccagcagg tcgagccgcc cgtggatatg
     1621 gtcgaccatg cgctgaaggc tgatcctttt gtggaagtac gccgcgttcg gaccggggca
     1681 gatggccacc gcttccagct tgtgcgccgg tttcatgccc gccttcagca gcgccccggc
     1741 cccgagccct tcgcaaagac agtccttggc ggtgaccgct gcctcttcgc gagccaatac
     1801 cttcgggtcg gtaatggttg agcgtaaccg ggccagcttc aggtgctggt actgccggga
     1861 ggccgtgcag atcggtcgtt ccgtgaattc ggtattgttg ctgaggaatt ccttgaaaca
     1921 gggactgccc ggtcggcctt tggcgatgcg ggtcgtccgc tgctgctcgg aactgctgtg
     1981 gcggaagttg tggaagggca cgcccaatgg ggacgcctgg ctgaggaagt agtcctcggg
     2041 cctggcctgg atcaactgtt ccagcgtggc gggatccacg ttggtggcct cgggcacgaa
     2101 gaggaagggg ctgccccaac cggtgcc
//
LOCUS       DAOQOC010000505         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33769_length_2121_cov_3.278316, whole genome shotgun sequence.
ACCESSION   DAOQOC010000505 DAOQOC010000000
VERSION     DAOQOC010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33769_length_2121_cov_3.278316"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PL106_08655"
     CDS             <1..489
                     /locus_tag="PL106_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33178.1"
                     /translation="VYGWPTIVDADHAIMVTGIAFSPVHVDSVIITHRSGPDGPQRIL
                     VSSGVNESMSDQQVSDLAVPGEFGTTVLTDLGDVLPGEGMAYGFFSLMLQPYFSEGGA
                     WEIDALRIVGSPAQSTGVTDLTMPTNMAPLPKFDALGRTIIDRPGLRFYLDGSKHVTL
                     GR"
     gene            860..>2121
                     /locus_tag="PL106_08660"
     CDS             860..>2121
                     /locus_tag="PL106_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33179.1"
                     /translation="MRIGVRSVILLVPIVSGIGSRGQEVVVEPKDWQTDATIINPEGL
                     WDKNASVAGQGSEVPGIDLTAGGHARHHLGDSAQFALRTYVDTAWASASSTKDSIVPG
                     ATVHWVRFHLVAGEAMKGQPLLLNIQVKAAFTLFLNGEQLLHASDMHSKHPWGRADPA
                     PRWSVPLKLLCDGDDEVLAMRLETWPGSTLGSTDLQASIHASGSGYHLNSIVMNYGVF
                     IGINVIILLMAMLMGWSEGRRRVWFLLAALSFVSVLDTVCVLGGTMGALGATRTTTHL
                     LALLRIITVPWSMYLLVLVLAELHGELSQKRKRRYRIGIAALTLGGLMTIGLVLWIVP
                     NDTSDFVFDGTGSPVLLAYFVVLALLAVVGLVALASIAVTEVRLGLKLWRSKGYERWV
                     GAGAVVASLLTLLLAAAGAVSGLALSRW"
BASE COUNT          381 a          633 c          684 g          423 t
ORIGIN      
        1 gtctatggtt ggcccaccat cgttgatgcc gatcacgcga taatggtcac cggcatcgcc
       61 ttcagcccgg tccatgtgga cagtgtgatc atcacgcatc gctcaggtcc ggatggcccc
      121 cagcggatcc tggtgagttc cggtgtgaac gaaagcatgt ccgaccaaca ggtttccgat
      181 ctggccgtgc ccggggaatt cgggaccacg gtgctgaccg atctgggtga tgtgctgcct
      241 ggcgaaggca tggcctatgg ctttttctcg ctgatgctgc aaccctactt cagcgaaggt
      301 ggagcatggg aaattgacgc gctgcgcatc gtgggcagtc cggcacaatc aaccggggtc
      361 accgacctca ccatgccgac gaacatggcg cccctgccca agttcgacgc gctcggccgg
      421 accatcatcg accgtccggg cctgcgcttc tacctggacg gcagtaagca tgtgaccttg
      481 ggccgataag gggcaccgga acccgatcat tggccgcacc ggcaaacccg atggagttgt
      541 tgtggttgat gtcgccacta tacaggtggt agaacgagcc gggaggtgct tgaggatcgg
      601 taccgaagac caacgaagcg gccgttgtgg atccgggcgg taccttgagg cggatgacgc
      661 ccagaacctg gttgttcaag atgagtaacg gagcaagcgc atcgatcagc gccatgcgtc
      721 aggagccctt cgaagctggt gatccgatca tgcaccggat cacatggaat gcggcacagc
      781 caaaaccgcg cacccattcg ccccggcgca ggagaaaccg cgcaaccggc atcttccctg
      841 cactgcctac atttccacca tgcggatcgg ggtgcgttcg gtgatcctat tggtgcccat
      901 cgtgtcaggc attggatcgc gcggccagga agtggtggtg gagccgaagg actggcagac
      961 cgatgcgacc atcatcaatc cggaaggact gtgggacaag aatgcgagcg tggcgggcca
     1021 gggatccgag gtacccggta tcgatctgac agcgggcggc catgcacggc accacctcgg
     1081 ggacagtgcc caattcgcct tgcgcaccta tgtggacacg gcgtgggctt cggcctcctc
     1141 cacgaaggac agcatcgttc caggcgccac cgttcattgg gtacgtttcc acctggtggc
     1201 cggtgaggcc atgaagggcc aaccgctgtt gttgaacatc caggtcaaag cggcattcac
     1261 cctgttcctc aacggggagc aactcctgca cgcgtcggac atgcacagca agcacccctg
     1321 gggccgggcc gatcccgcac cacgctggtc cgttccgctc aaactgcttt gtgatggcga
     1381 tgatgaagtg ctggccatgc gtttggagac ctggccggga tccacgctgg gctccaccga
     1441 cttgcaagca agtatccacg cctccggtag tggctaccat ttgaacagta tcgtgatgaa
     1501 ctacggcgtg ttcatcggca tcaacgtgat catcctgctc atggccatgc tgatgggctg
     1561 gtcggaaggg aggcgcaggg tctggttcct gctggccgcg ttgtccttcg tctcggtgct
     1621 cgataccgtt tgcgtgctgg gcgggaccat gggtgcgctg ggcgcgacaa gaacgacaac
     1681 ccacttgctg gccttgctgc ggatcatcac ggtgccctgg agcatgtacc tcctggtgct
     1741 ggtgctggcc gagctgcacg gcgaattgtc gcaaaagcgc aagaggcgct accggatcgg
     1801 gatcgcggcg ctcacactgg gcgggttgat gaccattggc ctggtcctgt ggatcgttcc
     1861 gaatgacaca tccgacttcg tatttgatgg taccgggtcg ccggtgctgc tggcatactt
     1921 cgtcgtgctg gccctcctgg cggtggtcgg actggtagcc ctcgcctcca tcgcggtgac
     1981 cgaggtgcgg ctgggcctca agctgtggcg ttccaaaggc tacgagcgct gggtgggagc
     2041 cggtgccgtg gtcgccagcc tgctcacctt gctgctcgcc gcggccggtg cggtatccgg
     2101 cttggccctg tcgcgttggt t
//
LOCUS       DAOQOC010000506         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_33846_length_2119_cov_2.577519, whole genome shotgun sequence.
ACCESSION   DAOQOC010000506 DAOQOC010000000
VERSION     DAOQOC010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33846_length_2119_cov_2.577519"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..172
                     /locus_tag="PL106_08665"
                     /pseudo
     CDS             26..172
                     /locus_tag="PL106_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
     gene            380..>2119
                     /locus_tag="PL106_08670"
     CDS             380..>2119
                     /locus_tag="PL106_08670"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNA33180.1"
                     /translation="MRILTLGTAAFLATTLSAQTLFTEDFEGNSPAFVLNTTDAGSIV
                     SSWNTWVINNSYNGGDGDLVCLGFPFGYSIVNTPAQPAGITSANGRYLHTASVEGIAD
                     GITNCSFGAADGFCINADNTFSRMSTDVNTVGAGSVDLKFWWICEGGSSYYGQVYYSV
                     DGGSSWTATSTPSQYNFQGSWTEQTVSLPAFAGQATLRFGFRFVNAVGSGATDPGFAI
                     DDVRIIADNTSSAAIVPGVVAPLSYCQGTDVDVPYTITGTFNPGNVFTAQLSDAAGSF
                     ALPVPIGSVASVTGGTIAAVIPPGTPQGTGYRIRVVSSSPVVTGADNGSNISILMGPN
                     AGTDASLTICEGDDPVLLSTGGDPDGTWSGPSPVVDTLYDPTSMDPGTYTYTVVSTGP
                     CGNDMATVVVTELAGADAGVSVTATICKNTGIHDLFALLGGAPDVGGTWTNPGGMPHT
                     GMFNSDFEQGGIFTYTVTGGGSCGNDEAVVAVTLGQPGQAGPDAVWTVCSSDLPVDLF
                     DLLVDANLTGVWHQDDEAFNGVATAAGDYVYVDFAQQPCSNDTAFITLVVTPAADAGT
                     NSTAQICPGDPAAD"
BASE COUNT          369 a          701 c          634 g          415 t
ORIGIN      
        1 atcctcgccg cccccctggc cccccctgca ccgcgtcctc acaggcattc caggcaccgg
       61 aacgccccac ctgggcagcc tgctgggcgc catcggcccg gccatcaccg tggggaacct
      121 ggcgggcaac gatgccttcc tgttcatcgc tgacctctct tccttatacc aaggtgcggc
      181 tcaactctgt gcgttcccgg tgccccgcgc gtggtccaca agccgcccag aaggaatacg
      241 ctccgcatga tgccgaatac atcggctcga ccaccgtgcc tgcgaacgga gccagttatg
      301 cgcgatcagg catggcatac tgttcgggtc gtccctctat cttgctcgcg ccgaacccag
      361 cctacggcat ccggacccca tgcgcatact tactcttgga acagcagctt ttctggccac
      421 gacgctctcg gcccagacct tgttcaccga ggacttcgag gggaattcac ccgccttcgt
      481 tctgaacacc accgatgccg gctccatcgt gagcagttgg aacacctggg tgatcaacaa
      541 cagctacaac gggggcgatg gtgacctggt ctgcctcggt ttcccgttcg gctattccat
      601 cgtgaacaca ccggcacaac ccgcgggtat caccagtgcg aacgggcgtt atctccacac
      661 cgccagcgtg gagggcatcg ccgatggcat caccaattgt tcattcggtg cggcggacgg
      721 cttttgcatc aatgccgaca acacgttcag ccgcatgagc accgatgtga acacggttgg
      781 tgccggcagc gtggacctga agttctggtg gatatgcgag ggtggctcca gttactatgg
      841 tcaggtttac tacagcgtgg atggcgggtc ctcgtggacg gccacctcca cgccctcgca
      901 gtataatttc caaggctcct ggaccgagca gaccgtgagc ctgccggcat tcgctggcca
      961 ggccaccttg cgcttcggat tccgcttcgt caatgcggtg ggctccgggg ccactgatcc
     1021 cggtttcgcc attgatgatg tgcgcatcat cgccgacaac acctcgtcgg cggccattgt
     1081 gccgggagtc gtggcgccgc tcagctattg ccagggcacg gatgtggatg ttccatacac
     1141 gatcaccggt acattcaacc cgggcaacgt gttcaccgcc caactgagcg atgcagcggg
     1201 cagtttcgcc cttccggtgc ccatcggttc ggtcgccagt gtcaccggtg gtaccatcgc
     1261 ggccgtgata ccacccggaa caccccaggg aacgggctac cgcatccggg tggtgtccag
     1321 ctctcctgtt gtgaccggtg cggacaatgg ctcgaacatc tccatcctga tgggccccaa
     1381 cgcgggtacc gatgcgtccc tgaccatctg cgagggcgat gatccggttc tgctcagtac
     1441 gggtggtgat ccggatggta cctggtccgg tccaagtccg gtggtggata ccctgtacga
     1501 tcccacttcc atggatcccg gcacctatac atacaccgtg gtaagtacag gcccctgcgg
     1561 caacgatatg gccaccgtgg tggtcaccga acttgcgggc gccgatgccg gcgtgagcgt
     1621 cacggcgacc atctgcaaga acaccgggat ccacgacctc ttcgcccttc tgggcggggc
     1681 cccggatgtc ggggggacgt ggaccaaccc gggcggcatg ccccacaccg gtatgttcaa
     1741 ctccgacttc gagcagggag gtatcttcac atacacggtc acgggtggtg gcagctgcgg
     1801 caatgacgag gcggtggtgg cggtcaccct gggccagccg ggccaggccg gtcccgatgc
     1861 ggtttggacc gtttgttcca gcgacctgcc cgtggatctg ttcgatctgt tggtggacgc
     1921 caacctgacc ggggtctggc accaggatga cgaggcgttc aacggagtgg ccaccgctgc
     1981 tggggattat gtctatgtgg acttcgcgca gcaaccgtgc agcaacgaca cggcgttcat
     2041 cacgctcgtc gttaccccgg ccgccgatgc gggtaccaac agcaccgctc agatctgtcc
     2101 gggtgatccc gccgccgac
//
LOCUS       DAOQOC010000507         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34057_length_2112_cov_6.765192, whole genome shotgun sequence.
ACCESSION   DAOQOC010000507 DAOQOC010000000
VERSION     DAOQOC010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34057_length_2112_cov_6.765192"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PL106_08675"
     CDS             <1..347
                     /locus_tag="PL106_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33181.1"
                     /translation="RWRIVVQRDTLAIGPLDDVRLWAGLRAAKNNEVRRCVWELHQQA
                     KEVALLRSALDLSKKSDKANAQLVGDLNEALVQCVDARARAERRAKRRSPFIWGISGL
                     AAGSLATLFLVR"
     gene            344..733
                     /locus_tag="PL106_08680"
     CDS             344..733
                     /locus_tag="PL106_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33182.1"
                     /translation="MKDWNPNDIVKWSNDQWNRCWSVVYQWTDGHARPTGTTIIEHGN
                     QRVEVKDSEIRALTFREFVWFEHPVTLHMMSGKTRGYMAIIIHVMTFLLTGAAEAQGF
                     SMVGLLCLLPVAGYWWGTWNNYTGKWV"
     gene            730..1146
                     /locus_tag="PL106_08685"
     CDS             730..1146
                     /locus_tag="PL106_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33183.1"
                     /translation="MTLIFLTACVLAALIAWVDANADAQHILAGHAINHADGLTERSV
                     LGAVIVLSLWAVAKHYGATVWHLLGLALAFAAAFSIVFRARLNKRRGLPLTYVSLSNV
                     YDSVFIRLFGARAWIAAYTFEALVMACGTIIYYLKP"
     gene            1150..>2112
                     /locus_tag="PL106_08690"
     CDS             1150..>2112
                     /locus_tag="PL106_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33184.1"
                     /translation="MSKKKTTPIKVTLKKGKHKTQPWTFAIDDIGPGPKVSVRERYAT
                     MKTAWKGALRKLKAWNGSYATPQHTATGRAIVRVLGVVLAYLFAGILSAQSLYVDVAA
                     KYGVTYADGSKRISDKYAAGKDTINTGLYWAWQRMKNAGMPYATFRASYLGWGIAQDV
                     AWTGEAPNNLKSGYQNTYSFGDWRGARGRWLMNRTAQIPRAGMEGASSFWYAGQGGDQ
                     GQVATEFVMDDTDWWPTNTTERRLFETPNSSLDGTNNFSYNESFRVERILLSGPEKND
                     GVRRIGLFLRRSGECTWVNQVRANDFSIGIAAYGGVPLTCGTITT"
BASE COUNT          421 a          585 c          750 g          356 t
ORIGIN      
        1 accgctggcg catcgtcgta cagcgcgata cgctggccat tgggcctctc gacgatgtgc
       61 gcttatgggc tgggctgcgt gcggccaaga acaacgaggt gaggcggtgc gtatgggagt
      121 tgcaccaaca ggcaaaggag gtggccttgc tgcgtagcgc actggacctg tccaagaaga
      181 gcgacaaggc caacgcgcag cttgtggggg atctcaacga ggccctcgtg cagtgcgtgg
      241 acgctcgtgc aagggccgag cgaagagcga agcgtcgatc tccgttcatt tggggcatct
      301 ccggccttgc ggctggatca ttggcaacgc tattcttggt cagatgaaag actggaaccc
      361 gaacgacatc gtgaaatggt ccaacgacca gtggaatagg tgctggtccg tggtgtacca
      421 gtggaccgac ggccatgccc ggcccacggg caccaccatc atcgagcacg gcaaccagcg
      481 ggttgaggtc aaggacagtg agatccgcgc cctgaccttc cgtgagttcg tatggtttga
      541 gcaccccgtc acgcttcaca tgatgagcgg caagacgcgg gggtacatgg cgatcatcat
      601 acacgttatg acattcctgc tcaccggggc ggcagaggca cagggtttca gtatggtggg
      661 acttctctgc ctgctcccgg tggcgggata ctggtggggg acgtggaaca actacaccgg
      721 aaaatgggta tgacgctgat cttcctgacg gcttgcgtgc tggctgcgct gatcgcgtgg
      781 gtggacgcca acgcggacgc gcagcacatc ctcgctggcc atgccatcaa ccatgcggac
      841 ggactgaccg agcggtcagt gctgggcgcg gtgatcgtgc tctcgctgtg ggcggtggcc
      901 aagcactacg gcgctacggt gtggcacctg ctggggctgg cccttgcgtt cgccgcagcg
      961 ttctccatcg tgttccgcgc ccggctgaac aagcggcgcg gtctgcccct gacctacgtg
     1021 agcctgagca acgtgtacga cagcgtgttc atccggctct tcggcgcgag ggcctggatc
     1081 gcagcgtaca cgttcgaggc gctggtgatg gcgtgcggca cgatcattta ctacctgaaa
     1141 ccctgaacca tgagcaagaa gaaaacgacc ccgatcaagg tgaccctgaa aaaagggaag
     1201 cacaagacgc agccgtggac gttcgcgatt gacgacatcg gccccggccc gaaggtgtca
     1261 gtgcgggagc ggtatgcgac gatgaagacg gcgtggaagg gggcactgcg caagctgaag
     1321 gcatggaacg gatcctacgc caccccgcag cacacggcca cgggccgcgc tatcgtgcgg
     1381 gtgctggggg tggtgctggc gtacctcttc gccgggatcc tcagcgccca aagcctgtac
     1441 gtggacgtgg cggcgaagta cggggtgacg tatgcggacg ggtcgaagcg gatcagcgac
     1501 aagtacgcgg cgggaaagga cacgatcaac acggggctgt attgggcgtg gcagcggatg
     1561 aaaaatgcgg ggatgcccta cgccaccttc cgggccagct acctcgggtg gggtatcgct
     1621 caggacgtgg cgtggaccgg tgaggcaccg aacaacctga agagcgggta ccagaacacc
     1681 tactcgttcg gggactggag gggtgcgcgg ggcaggtggc tgatgaaccg gacggcgcag
     1741 atcccacgtg cggggatgga gggggcgagt tcgttctggt acgccgggca gggcggggat
     1801 caagggcagg tggcgacgga gttcgtgatg gacgataccg actggtggcc gaccaacact
     1861 actgagcgac ggctattcga gacgcccaac agcagccttg acgggacgaa caacttcagc
     1921 tacaacgaga gcttccgggt ggagcgcatc ctgttgagcg ggccggagaa gaacgacggg
     1981 gtgcggcgca tcgggctatt tctacgcagg tcgggggagt gtacgtgggt gaaccaggtg
     2041 cgggccaacg acttcagcat cggcatcgcg gcatacggtg gcgtgcccct gacgtgcggg
     2101 acgatcacca cg
//
LOCUS       DAOQOC010000508         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34118_length_2111_cov_2.815175, whole genome shotgun sequence.
ACCESSION   DAOQOC010000508 DAOQOC010000000
VERSION     DAOQOC010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34118_length_2111_cov_2.815175"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PL106_08695"
     CDS             complement(<1..267)
                     /locus_tag="PL106_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNA33185.1"
                     /translation="MNVVIGQDIQRAAALLSEGELVAIPTETVYGLAANAFDADAVLR
                     IFQVKERPAFDPLIVHVHGMEQLERVVATVPPEAEALMHAFWPGP"
     gene            complement(264..1691)
                     /locus_tag="PL106_08700"
     CDS             complement(264..1691)
                     /locus_tag="PL106_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNA33186.1"
                     /translation="MRRAMDPERAQGEVLRMLIRTARDTAFGKEHRLSGVQDHASLAQ
                     AVPLHDYEGLRPYLDRVIAGEADVLWPGRPIYLSKTSGTTSGAKYIPITRQSMPHHIH
                     AARNALLAYIAGTGNTAFVDGKMIFLQGSPVLDRSGAIPSGRLSGIVADHVPAYLQRN
                     RMPTRATNTIPDWESKVEAIVTETMNADMRLISGIPAWTQMYFERLLARTGKRNVREV
                     FPAFSLFVYGGVNYAPYRDRMRALIGGEVDSIELFPASEGFIAWQDRRDDEGLLLNTA
                     SGIYFEFIPQEHGSAPAKRIPLAGVRVGVNYALVLHTNAGLWGYEIGDTVRFVSLDPP
                     RIVVTGRTKHFTSAFGEHVIAEEVEGALKDAMAGQPCEVSEFTVAPQTAPAEGGLPYH
                     EWFIEFAQPPRDLSRFAAVLDEAMMRRNVYYKDLITGSVLRPLVITSVERGGFNRWMK
                     ARGMNDAQSKVPRLANDRNYVEGLV"
     gene            complement(1838..>2111)
                     /locus_tag="PL106_08705"
     CDS             complement(1838..>2111)
                     /locus_tag="PL106_08705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009640898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA33187.1"
                     /translation="PPTIVTKPRSLLSSWTSDGGYVLHVQHAGNLVSVYKHNAVLLRK
                     QGDRVRAGEAIAIVGNSGELTTGPHLHFELWHRGEPVDPQAYMVFK"
BASE COUNT          354 a          690 c          674 g          393 t
ORIGIN      
        1 cggccccggc cagaacgcgt gcatcagtgc ctcggcttcc ggtggaacgg tggccaccac
       61 gcgctccaat tgctccatgc catgcacatg aacaatgagc ggatcgaagg ccggtcgctc
      121 cttcacctgg aagatgcgca gaacggcatc ggcatcgaag gcgttcgcgg ccaagccgta
      181 caccgtttcc gtggggatgg ccaccagttc accttcgctc agcagggctg ccgcgcgctg
      241 gatgtcctgt ccgatcacca cgttcatacc agtccttcca cgtagttgcg atcattggcc
      301 aggcgcggaa ccttgctttg ggcatcgttc atgccgcgcg ctttcatcca tcggttgaag
      361 ccgccccgtt ccaccgatgt gatgaccagt ggacgcagca cgctgcccgt gatcaggtcc
      421 ttgtagtaca cgttccggcg catcatggcc tcatcgagca ctgcggcgaa acgcgacagg
      481 tccctggggg gctgggcgaa ctcgatgaac cactcgtgat agggcaggcc cccttccgct
      541 ggtgcggttt gtggcgcaac ggtgaactcg ctcacctcgc aaggctgccc ggccatggca
      601 tccttcaagg cgccctccac ctcctccgcg atcacatgct caccgaaggc gctggtgaag
      661 tgcttggtgc gtccggtgac cacgatgcgc gggggatcga gcgatacgaa acgcaccgta
      721 tcgccgatct cgtagcccca caggcccgcg ttcgtgtgca gcacgagcgc ataattcacg
      781 ccgacccgca ctccggccag ggggatccgc ttcgcgggcg cactcccgtg ctcctggggg
      841 atgaactcga aatagatacc gctggccgtg ttcaacagca ggccctcatc atcacgccgg
      901 tcctgccagg cgatgaaacc ctcactggcg gggaacaact cgatgctgtc cacctcccca
      961 ccaatgagcg cgcgcatgcg gtcgcggtag ggcgcatagt tcacaccgcc gtacacgaag
     1021 agcgaaaaag cggggaacac ctcccggacg ttgcgcttgc ccgtacgtgc gagcaggcgc
     1081 tcgaagtaca tctgcgtcca cgccggtatg ccgctgatca ggcgcatgtc cgcgttcatc
     1141 gtttcggtga cgatggcctc caccttgctc tcccagtcgg ggatggtgtt cgtggccctg
     1201 gtcggcatgc ggttgcgctg gaggtacgcc ggcacatggt cggccacgat gccgctcagc
     1261 cgaccgctgg ggatggcccc actgcgatcg agcacggggc tgccctgcag gaagatcatt
     1321 ttgccatcca cgaaggcggt gttgccggta ccggcgatgt aggcgagcag ggcgttgcgc
     1381 gccgcgtgga tgtggtgcgg catgctctgc cgggtgatcg gtatgtattt cgctccactg
     1441 gtggtgccac tggtcttgga caggtagatg gggcgacccg gccacagcac gtcagcctcc
     1501 ccggcgatca cacgatcgag gtatggtcga aggccttcat agtcatgcag cggcacggcc
     1561 tgggcgagtg aggcatggtc ctgcaccccg ctcagccggt gctccttgcc gaaagccgta
     1621 tcgcgtgcgg tgcggatcag catgcgcagc acctcgccct gggcccgttc gggatccatt
     1681 gcgcggcgca tgacccgggc cgttatccac cgggcgtagg ggagactgag agcggccttg
     1741 atacccacgt gctatcggtt ctggggatgg atcccgacca ctggaaggtc cgtgcccgga
     1801 agccggtccg aaagcgacct gtgcagcgcc accgcggtca tttgaacacc atgtaggctt
     1861 gcgggtccac cggttcgccg cgatgccaga gttcaaaatg caggtgcgga ccggtggtga
     1921 gttccccgct gttgcccacg atggcgatgg cctcgcccgc ccgcacccgg tcgccctgct
     1981 tgcgcagcag caccgcgttg tgtttgtaca cgctcaccag gttgcccgca tgctgtacgt
     2041 gcagcacata gcccccatcg ctggtccagc tgctgagcag gctgcgtggc ttggtgacga
     2101 tcgtcggggg g
//
LOCUS       DAOQOC010000509         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34228_length_2108_cov_3.004384, whole genome shotgun sequence.
ACCESSION   DAOQOC010000509 DAOQOC010000000
VERSION     DAOQOC010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34228_length_2108_cov_3.004384"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PL106_08710"
     CDS             <1..506
                     /locus_tag="PL106_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA33188.1"
                     /translation="TGVRSDDVRGRSFAKRGHTPIVRVGNKCFGRSIISAVSARGGMR
                     SMVFKGVLDVRCFKRFLSRSIKDAKRKVFLIVDDLRVHHAKVLAPWLLKHKERIEIFY
                     LPSYFPEINPVEVAKADLKREMGDKPPAQNEEQMVRAILAHYHNVQRQPLRILKYFQH
                     PDVRYAA"
     gene            689..1234
                     /locus_tag="PL106_08715"
     CDS             689..1234
                     /locus_tag="PL106_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA33189.1"
                     /translation="MASDERSAFMQAYEPCHAAFARYCAALAYGRMDVQDLMQDVLLS
                     AFQRFDHIRKKDELLHYLMRAARNRMVSSLRISRRNEAITSKQTERLLERGATAEQLT
                     DVGILHQAINKLPTAQRDALVLFEVAGLSMAEIAAIQGSNENAVKTRVSRARAAVRSM
                     LDGRTKPLRMELLRSLLILLP"
     gene            1231..>2108
                     /locus_tag="PL106_08720"
     CDS             1231..>2108
                     /locus_tag="PL106_08720"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNA33190.1"
                     /translation="MNRAEEEQAVLAPLRELPPEVSLEQVRDMVAAFPLGMGAVAWLI
                     HTLKFNLNTLLMTSIGTLLLGTGLYLFSGSTPAATPVPNGLPRRNDTVVRTPAVPAPA
                     IAPATPTLARPEQGSSVVPAADRTIEDLHVETGTEAPKETPGSGTPVQEATAPMPEPT
                     TVTTGVANERIFNLSGFKGIALRSSSDVRVEEGEFAVVAKGEQQHLDMLDIKVDQGIL
                     SIEHEARRRMDLGMEKVLITVRLPRLDQLIVHGSGSIHVAEFKNTPSLQLSVMGSGDI
                     DMAGPIETTEMNIQVQG"
BASE COUNT          456 a          662 c          609 g          381 t
ORIGIN      
        1 agaccggcgt gcgtagtgac gatgtgcgcg gacgcagctt cgccaagcgc gggcacacgc
       61 ccatcgtgcg cgttggcaac aagtgcttcg ggcgctcgat catctcggcg gtgagcgcac
      121 gcgggggcat gcgctcgatg gtcttcaagg gcgtgctgga cgtgcgctgc ttcaagcgat
      181 tcctctcccg ctcgatcaag gatgccaagc gcaaggtgtt cctgatcgtg gacgatctcc
      241 gggtgcacca cgccaaggtc ctcgcccctt ggctgctcaa gcacaaggag cgcatcgaga
      301 tcttctactt gcccagctac ttcccggaga tcaaccccgt ggaggtggcc aaagccgacc
      361 tcaagagaga gatgggagac aagccgccag cacagaacga agaacaaatg gtaagggcca
      421 tcctggcaca ctaccacaat gttcagcgac agcccctgcg catcctcaag tacttccagc
      481 acccggacgt tcgctacgcc gcatgagtac ggatcttcat cccctgctca gtaattggcc
      541 caagtcacga atgaaaccgt cgaccgcaca ggaccctcca gcattcatcc acataccccg
      601 cgctgagcag gatcaccagt gatcacaacc agcttgtaac ctttatcccc tttctgcgac
      661 tctctgccag gaaaccaagt cctgcaccgt ggcctccgac gagcgttccg ccttcatgca
      721 agcctatgag ccgtgccacg cggccttcgc acggtattgc gctgcactgg cctacgggcg
      781 catggatgtg caggacctga tgcaggacgt cctgttgagc gcattccaac ggttcgacca
      841 tatccgtaaa aaggatgaac tgctgcacta tctgatgcgc gcggcgcgca accgcatggt
      901 gagctccttg cgcatcagcc gaaggaatga ggccatcacc agcaagcaga ccgaacggtt
      961 gttggagcgg ggtgccacgg ccgaacaact caccgacgtg gggatcctgc accaggccat
     1021 caacaaactg cccaccgctc aacgggatgc tctcgtgctc ttcgaagtgg ccggcctgtc
     1081 catggcggag atcgccgcta ttcaaggcag taatgagaat gcggtgaaaa ccagggtgag
     1141 tcgtgcgcga gcggccgtcc gaagcatgct ggatggtcgc accaaaccct tacgcatgga
     1201 gctcctccga tcactattga tcctgttgcc atgaaccgcg cagaggaaga acaagccgtg
     1261 ctcgccccgt tgcgtgaact gcccccggag gtcagcttgg aacaggtgcg ggacatggtg
     1321 gcggcattcc ccttgggcat gggcgccgtg gcctggctga tccatacgct gaaattcaac
     1381 ctgaacacct tgctcatgac cagcataggc actcttctcc ttggcaccgg cctctacctc
     1441 ttttcaggaa gcacacccgc agccacgccg gtccccaatg gccttccacg aaggaatgat
     1501 accgtggttc gaacaccggc cgtgccagca cctgccattg ctcccgccac gccgacgttg
     1561 gcccgacccg agcaaggatc gtccgtcgta cctgctgcgg accggacgat tgaagacctc
     1621 cacgtggaaa cggggaccga ggcgccgaag gaaaccccgg gatccggaac tcctgtacaa
     1681 gaggccacgg caccgatgcc agagccgacc acggtgacca cgggtgttgc caacgagcgc
     1741 atcttcaacc tgtccggctt caagggcatc gcattgcgat ccagttccga tgtgcgtgtg
     1801 gaggaaggtg aattcgccgt ggtggccaaa ggcgaacaac agcatttgga catgctcgac
     1861 atcaaggtgg accaagggat cctgtccatc gaacacgaag ctcggcgccg tatggacctg
     1921 ggcatggaga aggtgttgat caccgtgcgg ctcccgcgct tggaccaatt gatcgtgcat
     1981 ggttcgggca gcatccatgt ggcggagttc aagaacacgc cttcgcttca actctccgtg
     2041 atgggctcgg gtgacatcga tatggcgggt ccgatcgaaa ccacggaaat gaacatccag
     2101 gtgcaggg
//
LOCUS       DAOQOC010000510         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34424_length_2102_cov_4.949194, whole genome shotgun sequence.
ACCESSION   DAOQOC010000510 DAOQOC010000000
VERSION     DAOQOC010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34424_length_2102_cov_4.949194"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /gene="nrfD"
                     /locus_tag="PL106_08725"
     CDS             complement(<1..928)
                     /gene="nrfD"
                     /locus_tag="PL106_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNA33191.1"
                     /translation="MHSESSLREPLILGHRTYHDITVDLVTPIEEKAPRMWYILLTIA
                     AIIAVYGVGCILYLFSKGIGTWGLNKTVDWAWDITNFVWWVGIGHAGTLISAVLLLFR
                     QKWRMAVNRSAEAMTIFAVIMAAVFPMIHMGRIWMAYWVFPLPNQFGSLWVNFNSPLL
                     WDVFAISTYFTVSLVFWYIGLIPDFATIRDKVVRPWQKTFYGILSFGWTGNAKAWQRF
                     EEVSLVLAGLSTPLVFSVHSVVSFDFATSVIPGWHTTIFPPYFVSGAVFSGFAMVQTL
                     LLVMRKTLKLEAYVTIKHIEYMNIIIIVTGSIV"
     gene            complement(933..>2102)
                     /locus_tag="PL106_08730"
     CDS             complement(933..>2102)
                     /locus_tag="PL106_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNA33192.1"
                     /translation="GKSAYPMTRIANGTVSYSAVNGTLENTGARYEMGLTQTQLTGMD
                     RHSIIKETTIGTWRAHDPRGAKDSKNGYNEKVMLAVHEDVNHDGHIDARDKVPATEVD
                     LWGDHPVEQVGHHWGMSIDLNACNGCGACITACHSENNVPVVGKDEVRRSRDMHWLRL
                     DRYYSSDMTHEVGKAQGMGKIDRYLAMEEPSASPSVFFMPVMCQHCDHAPCETVCPVA
                     ATSHSNEGLNMMAYNRCIGTRYCANNCPYKVRRFNWFNYVTEQFGEVNPAWDELGRLV
                     LNPDVVVRSRGVIEKCSLCVQRIQEVKLTAKKAGRPVKDGEVQTACSTACGTGAIVFG
                     DLNDANSKVAARFKDERAYFMLEEIGVKPNVSYLAKVWNREEPPAIAAVTEEAHH"
BASE COUNT          374 a          666 c          633 g          429 t
ORIGIN      
        1 cgacgatcga accggtgacg atgatgatga tgttcatgta ctcgatatgc ttgatggtga
       61 cgtaggcctc aagcttcaag gtcttgcgca tcaccagcag caaggtctgc accatggcga
      121 aaccgctgaa cacggccccg ctcacgaagt agggagggaa gatggtggtg tgccagcccg
      181 ggatcaccga ggtggcgaag tcgaacgaca ccaccgagtg gaccgagaac accagtgggg
      241 tgctcagtcc ggcaagcacc aggctcacct cctcgaagcg ctgccaggct ttggcgttgc
      301 ccgtccagcc gaagctcagg atgccgtaga aggtcttctg ccagggcctc accaccttgt
      361 cgcggatggt ggcgaaatcg gggatgagac cgatgtacca gaacaccagc gataccgtga
      421 aatacgtact gatggcgaat acgtcccaca gcagcgggct gttgaagttc acccacagcg
      481 atccgaactg gttgggcagg gggaacaccc agtaggccat ccagatgcgg cccatgtgga
      541 tcatggggaa aacggccgcc atgataacgg cgaagatggt catggcctcg gccgaccggt
      601 tcacggccat gcgccacttc tgccggaaga gcaacaacac cgcgctgatc agcgtgccgg
      661 cgtgaccgat acccacccac cacacgaagt tggtgatgtc ccaggcccag tccacggtct
      721 tgttcagacc ccaggtcccg atccccttgc tgaagagata gaggatgcag cccaccccgt
      781 acaccgcgat gatggccgcg atcgtgagca ggatgtacca catccggggc gccttttcct
      841 cgatgggcgt caccagatcg acggtgatgt cgtggtaggt gcgatgcccc aggatgagcg
      901 gttcgcggag cgatgattcg ctgtgcatgg tcctagtggt gggcttcttc ggttacagcg
      961 gcgatggcgg gcggctcctc gcggttccac accttggcca ggtagctcac attgggcttt
     1021 actccgatct cttccagcat gaagtaggca cgctcgtcct tgaaacgggc tgccacttta
     1081 ctgttcgcgt cattcaggtc gccgaagacg atcgccccgg ttccacaggc ggtgctgcac
     1141 gccgtttgga cctccccgtc cttgaccggg cgacccgcct tcttggcggt gagtttcact
     1201 tcctggatcc gctgcacgca gagggagcat ttctcgatga ccccgcggct gcgtaccacc
     1261 acgtcgggat tcagcaccag acggcccagc tcatcccaag cggggttcac ttcgccgaac
     1321 tgttcggtca cgtagttgaa ccagttgaaa cggcggacct tgtacgggca gttgttcgcg
     1381 cagtaccgcg tgccgatgca acggttgtac gccatcatgt tcagcccctc gttgctgtgt
     1441 gaggtggcgg ctaccgggca tacggtttcg cagggcgcat gatcgcagtg ctggcacatc
     1501 accggcatga agaacacgct gggcgaggcg ctgggctcct ccatggccag gtaccggtcg
     1561 atcttgccca tgccttgcgc cttgcccacc tcgtgggtca tgtcgctgct gtagtaacgg
     1621 tccaagcgca accagtgcat gtcgcggcta cggcgcacct cgtccttgcc cactaccggc
     1681 acgttgttct cgctgtggca ggccgtgatg cacgcaccgc aaccgttgca ggcgttcagg
     1741 tcgatgctca tgccccagtg gtggcccact tgctccaccg ggtggtctcc ccagaggtcc
     1801 acctccgtgg ccggcacctt gtcgcgcgca tcgatatgcc cgtcgtggtt gacgtcctca
     1861 tgcacggcca gcatcacctt ctcgttgtac ccgttcttgc tgtccttcgc accgcgcggg
     1921 tcgtgcgcac gccacgtacc tatggtggtc tccttgatga tgctgtgccg gtccatgccg
     1981 gtgagctggg tctgcgtaag tcccatctcg taccgggccc ccgtgttctc caacgttccg
     2041 ttcacggcgc tgtagctcac cgtgccgttc gcgatcctcg tcatcgggta ggcactcttc
     2101 cc
//
LOCUS       DAOQOC010000511         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34544_length_2099_cov_2.712818, whole genome shotgun sequence.
ACCESSION   DAOQOC010000511 DAOQOC010000000
VERSION     DAOQOC010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34544_length_2099_cov_2.712818"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1459
                     /locus_tag="PL106_08735"
     CDS             74..1459
                     /locus_tag="PL106_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNA33193.1"
                     /translation="MTLGPMSQPRVHFIAIGGSAMHNLAIALHRKGYDVTGSDDEIFE
                     PSRSRLERLNLLPAQLGWHPEDITSDLQAVILGMHARKDNPELVRALELGIPVYSYPG
                     YFHEQTKDKTRVVIGGSHGKTTITSMIVHVLRTANADFDYLVGAQLDGFDCMVRLDPA
                     SRVAIIEGDEYLASALEPVPKFHLYKPDIALISGIAWDHINVFKTFESYVDQFRGFIK
                     AIEPGGKLVYCAEDPEVVRIVNEHKGDLIKVPYGVPPHTIRDGATYLITENGEVPLRV
                     FGRHNLMNLEGARHVCHQLGIGDPLFYEAIATFHGAAKRLEKLAEGPGRVVFKDFAHS
                     PSKLKATVDAVREQFPDRRLTACMELHTYSSLSEGFLDQYQGCMDAADEAIVFYDPHA
                     VQLKRLPPVPPERIHRAFGRDGLRVLNDPVAVMGAVRSSADVPGVLLMMSSGNFGGID
                     LQATAEAFIAP"
     gene            complement(1516..2001)
                     /locus_tag="PL106_08740"
     CDS             complement(1516..2001)
                     /locus_tag="PL106_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33194.1"
                     /translation="MTMNAPLLFSAFAIALRTGLFYAGIELEGFTFGFVLLGLVTVLV
                     FLCGHFTLNEEPNAPLPMIFRAGLQGAAVFALVYALFLLLFFKVLNTAEFPMRVAGLI
                     HDSTAQGVPEEEARRNINAFFTPGRYAFTTFAGLLGVGTVNALVFAVLQHKVLRRFRN
                     R"
BASE COUNT          418 a          646 c          678 g          357 t
ORIGIN      
        1 agcagcccgg gatatgtcgt tgggtgcctt gccttgcgcc cacctttgcg tgcggtcatg
       61 ggcacatgac cacatgaccc tcggccccat gtcccaaccc cgtgtccatt tcatcgccat
      121 cggcggcagc gccatgcaca acctggccat cgcgttgcac cgcaaaggct acgatgttac
      181 cggcagcgac gacgagatct tcgaaccgag ccggagccga ctggagcggt tgaacctgtt
      241 gcccgcgcaa ctgggctggc atccggagga catcacatcg gacctgcagg ccgtgatcct
      301 gggcatgcac gcgcgcaaag acaaccccga actggtgcgc gcgctggagc tggggatccc
      361 ggtatacagt tatccgggct acttccacga gcagacgaag gacaagaccc gcgtggtgat
      421 cggcggcagc cacggtaaga ccaccatcac cagcatgatc gtacacgtgc tgcgcaccgc
      481 gaacgcggat ttcgattacc tggtgggggc gcagctcgat ggtttcgact gcatggtgcg
      541 gctggatccc gcgagccgtg tggccatcat cgaaggggac gagtacctcg cttcagcgtt
      601 ggagcccgtg ccgaagttcc acttgtacaa gccggacatc gcgttgatca gcggcatcgc
      661 ctgggaccac atcaacgtgt tcaagacctt cgagagctac gtggaccagt tccgcggctt
      721 catcaaggcc atcgaacccg gtggcaagct ggtgtactgc gcggaggatc ccgaggtggt
      781 gcggatcgtg aacgagcaca agggtgatct gatcaaggtg ccgtacggcg taccaccgca
      841 taccatccgc gacggggcaa cctacctgat caccgaaaat ggggaggttc cgctgcgcgt
      901 cttcggtcgc cacaacctga tgaacctgga gggcgcccgt catgtgtgcc accagttggg
      961 catcggcgac ccgctgttct acgaggccat cgccaccttc cacggcgcgg ccaaacgcct
     1021 ggagaagttg gcggaagggc cgggccgtgt ggtcttcaag gatttcgcgc attcgcccag
     1081 caagctcaag gccacggtgg atgccgtgcg cgagcagttc ccggacaggc gcctcacggc
     1141 atgcatggag ctgcacacgt acagcagcct gagcgagggt ttcctggacc agtaccaggg
     1201 ctgcatggat gcggccgatg aggccatcgt cttctacgac ccgcatgccg tgcaattgaa
     1261 gcggctgcca ccggtgcccc cggagcgtat ccaccgcgct ttcgggcggg acggactgcg
     1321 cgtgctgaac gatcccgtgg ccgtgatggg cgcagtgcgc tcaagtgccg atgtgccggg
     1381 tgtattgttg atgatgagca gcggcaactt cggcggcatc gatctgcaag ccacggccga
     1441 ggcgttcatc gcaccgtaag aagggcatcg tccgtacctg tccgatcacc accacgaggg
     1501 atcggtcgaa tgtcctcacc ggttgcggaa cctgcgcaac accttgtgct gcagcacggc
     1561 gaagaccagc gcattcacgg tacccacacc cagcagacca gcgaaggtgg tgaaggcgta
     1621 acgccccggg gtgaagaagg cattgatgtt gcgacgcgcc tcctcttcgg gaacgccttg
     1681 tgccgtactg tcgtggatga ggccggccac gcgcatgggg aactcggccg tgttcaggac
     1741 cttgaagaag agcagcagga agagcgcata gaccagggcg aagacggcgg caccctgcag
     1801 gcccgcgcgg aagatcatgg gcagcggtgc gttcggttcc tcgttcagcg tgaagtgccc
     1861 gcagaggaag accagcaccg tgaccaggcc cagcaacacg aagccgaacg tgaagccctc
     1921 cagttcgatc cccgcataga agagtccggt acgcagtgcg atggcgaagg cgctgaagag
     1981 gagcggtgca ttcattgtca tggcggaggc gagaggtgag aggcgagagg cggcgtgctg
     2041 gccatagcgg acccctggcc tctcgcctct cgcctctcac cattcaacca ttcatggat
//
LOCUS       DAOQOC010000512         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34553_length_2099_cov_2.043053, whole genome shotgun sequence.
ACCESSION   DAOQOC010000512 DAOQOC010000000
VERSION     DAOQOC010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34553_length_2099_cov_2.043053"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PL106_08745"
     CDS             complement(<1..252)
                     /locus_tag="PL106_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA33195.1"
                     /translation="MWKNNLLIAWRTLKRDKLFAALNILGLAIGITACFLIYIYVQDE
                     LSFDAHHAKADRIHRVQAHYTFGDTQDDFGITPFPIVEAL"
     gene            complement(261..956)
                     /locus_tag="PL106_08750"
     CDS             complement(261..956)
                     /locus_tag="PL106_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA33196.1"
                     /translation="MSLLKIQSLSKIYRTDTVETTALSNVSIDIEQGEFVAIMGPSGC
                     GKSTLLNIIGLLDSPSSGQYFVHGEDVSGYNERKRAEVRKTTIGFVFQSFNLIDELTV
                     AENIELPLIYTRLGKAERKQRVQEAMDRMNIAHRAKHFPQQLSGGQQQRVAIARAVVN
                     APKLILADEPTGNLDSAMGEEVMGLLTELNKAGTTVIIVTHSLRDAGFAQRTINLLDG
                     SVLRAEVSTQVIH"
     gene            complement(1016..>2099)
                     /locus_tag="PL106_08755"
     CDS             complement(1016..>2099)
                     /locus_tag="PL106_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA33197.1"
                     /translation="RSALDTLRPAPAATAGPEAGEQPAVVLFTSGSEGVPKGVELTHH
                     NLLANIRQMLSVIDLTDDDRVFNALPIFHSFGLTVGTMLGLVRGVYVFLYPSPLHYRI
                     VPSALYDRYCTVFLATNTFLNNQEQLVYMREKRKLLAANVRSDSVFRQSQLGSITSNL
                     DLIQQNLRFLRENLQNLVIKAPIDGQLSGLNVELGQTKQKGERIAQIDVLGSFKVRAR
                     IPEHYVSRVSMGLLGTFTHAGKEHSIEVFKVYPEVSNGEFDVDLRFTGAQPVDIRRGQ
                     TFQVRLQLSEDLQAVMLPRGPFFQDTGGQWVYVVDAEGKAVKREVKLGRQNPDMYEVL
                     EGLRPGDHVVTSRYAAFNDADELVIQ"
BASE COUNT          370 a          590 c          685 g          454 t
ORIGIN      
        1 cagggcctcc acgatgggga agggcgtgat gccgaagtcg tcctgcgtat cgccgaaggt
       61 gtagtgggct tgcacccggt ggatgcggtc cgccttcgca tggtgggcat cgaagctcaa
      121 ctcatcctgt acgtagatgt agatgaggaa gcaggccgtg atgccgatgg ccagaccgag
      181 gatgttgagc gcggcgaaga gcttgtcgcg cttgagggtg cgccaggcga tgaggaggtt
      241 gttcttccac atggccagga tcaatggatc acctgtgtgg acacttcggc tcgcagcaca
      301 ctaccgtcca acaggttgat ggtgcgttga gcgaagcccg catcacgcag gctgtgcgta
      361 acgatgatca cggtcgtccc cgccttgttc agttcggtga gcaggcccat cacctcttca
      421 cccatggcgc tgtccaggtt gcccgtgggc tcgtccgcaa ggatcaattt gggcgcgttc
      481 accacggcgc gtgcaatggc cacgcgttgc tgttgcccac cgctgagttg ctgtgggaag
      541 tgcttggcgc gatgtgctat gttcatgcgg tccatggcct cctgcacacg ttgtttgcgt
      601 tcggccttgc ccaagcgcgt gtagatcaag ggcagttcaa tgttctcagc caccgtgagt
      661 tcatcgatca ggttgaagct ctggaacacg aaaccgatgg tggttttgcg cacctcggcg
      721 cgtttgcgct cgttgtagcc actcacgtcc tcgccgtgta cgaagtactg tccgctgctg
      781 ggcgagtcaa gcaagccgat gatgttgagc aaggtggact tcccgcatcc gctcggcccc
      841 atgatggcga cgaactcgcc ttgctcgatg tcgatgctca cgttgctcag cgcagtggtc
      901 tccacggtgt cggtgcggta gatcttggag aggctctgga ttttcaggag ggacatggtt
      961 agtgaggttg ctgattcgcg ggttacaggt tgcgggttgt acagaccgag ggtgctcatt
     1021 ggatcactag ctcatccgcg tcattgaatg cggcgtatcg actggtcaca acatgatcgc
     1081 cgggtcgcag gccctccagc acttcgtaca tgtccgggtt ctggcggccg agtttcacct
     1141 cgcgtttcac ggccttacct tcggcatcca ccacgtacac ccattgtccg ccggtatcct
     1201 ggaaaaatgg tccacggggc agcatcacgg cctgcaagtc ctcactcaat tgcaggcgca
     1261 cttggaaggt ctgcccgcgc cggatgtcca ccggttgcgc acccgtgaaa cgcaggtcca
     1321 cgtcgaactc gccgttgctc acctcggggt acactttgaa cacctcgatg ctatgctctt
     1381 tgcccgcgtg cgtgaacgtg ccgagcaggc ccatgctcac tcggctcaca tagtgttccg
     1441 ggatccgtgc acgcaccttg aaactcccca gcacatcgat ctgtgcgata cgctctccct
     1501 tctgtttggt ctggcccagt tccacattca gaccgctgag ctgcccatcg atgggcgctt
     1561 tgatcaccag gttctgcagg ttctcgcgca ggaagcgcag gttctgctgg atgaggtcga
     1621 ggttgctggt gatggagccg agctggctct ggcggaacac ggaatcgcta cgcacgttcg
     1681 cggccaatag cttccgtttt tcgcgcatgt acacaagctg ctcctggttg ttgaggaagg
     1741 tgttggtggc cagaaagacg gtgcaatagc ggtcgtagag cgcgctgggc acgatgcggt
     1801 agtgcagcgg cgacgggtaa aggaagacgt acacgccgcg gacgagaccg agcatggtgc
     1861 cgacggtgag gccgaagctg tggaagatgg ggagcgcgtt gaagacgcgg tcgtcgtcgg
     1921 tgagatcgat gaccgaaagc atctggcgga tgttggcgag caggttgtgg tgggtgagtt
     1981 cgacgccctt gggaacgccc tcggaaccgc tggtgaacag cacgacggcc ggctgctcgc
     2041 cggcttcggg accggcggtt gcggccgggg cgggacggag cgtgtcgagc gcggaacgc
//
LOCUS       DAOQOC010000513         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34789_length_2091_cov_3.885069, whole genome shotgun sequence.
ACCESSION   DAOQOC010000513 DAOQOC010000000
VERSION     DAOQOC010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34789_length_2091_cov_3.885069"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1578
                     /locus_tag="PL106_08760"
     CDS             193..1578
                     /locus_tag="PL106_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA33198.1"
                     /translation="MTSRRHFLRSLGLATTGAAFLPAYLRAAAPVKYDRVRVGVIGCG
                     LRAQEHLRECTLRNDVDVIALADPDPAMIARSQKTLRDGGHKEAVVYGNGNEDYLNLL
                     KRDDIDAVFICSPWEWHVPQGIAALEAGKIVGMEVCGGMNLQECWDIVRASERTGVPL
                     MMLENVCYRRDVLAVLNMVRQGLFGELVHGQGGYEHDLRNVIFNSGKEDGYGDGVEFG
                     AKGWSEAKWRTQHYVDRNGELYPTHGLGPVAVMMDINRGNRLTRLSSIASNARGAHKY
                     ILEHPKGGPDHPNAKVVFKCGDIVNTQIQTANGQTILLTHDTTLQRPYNLGFRVQGTE
                     GLWQDTGWGESDQGMVYFEKKMAHSHRWDNSKSWMEQFDHPLWKRYATMAEGAGHGGM
                     DYFMSHAFFECIKREQPFPLDVYDMATWYAITPLSEKSIAEGGQVQDIPDFTAGKWRE
                     RKPVFGFTDEY"
     gene            1731..1904
                     /locus_tag="PL106_08765"
     CDS             1731..1904
                     /locus_tag="PL106_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33199.1"
                     /translation="MLDALDLPAHEVNADDPIAQNTVWVRQQWDTARLLVKEGGRQGA
                     VLVIDEVQKLPSR"
     gene            1901..2047
                     /locus_tag="PL106_08770"
     CDS             1901..2047
                     /locus_tag="PL106_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33200.1"
                     /translation="MKSGSAFKRLPGMAAFHEAFEPARSLLIGPGGIDLEVFLEKPAS
                     AVLA"
BASE COUNT          433 a          628 c          682 g          348 t
ORIGIN      
        1 gtcagaatga accatggggg tgcgaaagtg cgagggtgcg agagtcacgt ctctcacacc
       61 ctcgcaccct tgaaccggga ccgaccggtt ctgtctcgcc gtctaagcaa gacgcgatag
      121 ccctggcggt agcccgaccg gcctaccggg atcgcacaac catgatttac ttcaccacgt
      181 gaacccgcaa ccatgacctc ccgccgccac ttcctccgct ctctgggcct ggccaccacc
      241 ggtgcggcct tccttccggc ctatctgcgt gccgcagcac ccgtgaagta cgaccgggtg
      301 cgcgtgggcg tgatcggttg cggccttcgc gcccaggaac acctgcgcga atgcaccctc
      361 cgcaacgacg tggacgtgat cgccctggcc gaccccgacc ccgccatgat cgcccgctcg
      421 cagaagaccc tgcgcgacgg aggccacaaa gaagcggtag tatatgggaa cggcaacgag
      481 gactacctga acctcttgaa gcgcgacgac atcgatgcgg tgttcatctg ctccccatgg
      541 gaatggcacg tgcctcaagg catcgctgcg ctcgaagcgg ggaagatcgt gggcatggag
      601 gtgtgcggcg gcatgaacct ccaggagtgc tgggatatcg tacgggccag cgagcgcacg
      661 ggcgttccat tgatgatgct ggagaacgtg tgctaccgtc gcgatgtgct tgccgtgttg
      721 aacatggtgc gtcaagggct cttcggtgag ctggtgcatg gccagggcgg ctacgagcac
      781 gacctgcgca acgtcatctt caacagtggc aaggaggacg gctacggcga tggggtggag
      841 ttcggtgcca aaggctggag cgaggcgaag tggcgtacac agcactatgt ggaccgcaat
      901 ggggagttgt accccacgca cggcctcggg cccgtcgcgg tgatgatgga catcaaccgg
      961 ggcaaccggc tcacccggct cagttccatc gccagcaacg cgcgcggagc gcacaagtat
     1021 attttggagc atcccaaggg cgggccggac caccccaacg ccaaggtggt gttcaaatgc
     1081 ggcgacatcg tgaacacgca gatccagacc gccaacggac aaacgatcct gctgacgcac
     1141 gacaccacct tgcagcgacc gtacaacctg ggcttccgcg tgcagggtac cgagggcctc
     1201 tggcaggaca ccggatgggg cgaaagcgac cagggcatgg tctacttcga gaagaagatg
     1261 gcgcacagcc accgctggga caacagcaag agctggatgg aacagttcga tcacccgctg
     1321 tggaaacgct atgccaccat ggccgagggc gcggggcacg gtggcatgga ctacttcatg
     1381 tcccacgcct tcttcgagtg catcaaacgc gagcagccct tcccgttgga cgtgtatgat
     1441 atggccacgt ggtacgcgat cacgccgctc agcgagaaga gcatcgccga aggcggccag
     1501 gtacaggata tccccgattt cacagcgggc aagtggcggg agcggaagcc ggtgttcggg
     1561 ttcaccgacg agtattgacc gggaggggtc ccggatctgc tcaatgcact gagtaagttt
     1621 actcaatgca ttgagtagta tttccacacg gggtacatcc gcccccagcg tgcgacgctg
     1681 ctcagaaggc cggccccgga caggagggca agcgcaccct ggtacgccag gtgctggatg
     1741 cactggacct gcctgcacat gaggtgaacg ccgatgatcc gatcgcacag aacacagtgt
     1801 gggtacgcca acagtgggac accgcacgct tgctggtcaa ggagggtggc cggcaaggtg
     1861 ccgtgctggt gatcgacgag gtccagaaat taccatcgag gtgaagagcg gctccgcatt
     1921 caagcgtctg cccggcatgg cggccttcca cgaagcgttc gagccagcgc gctcgttatt
     1981 gatcggaccg ggcgggatcg atctggaagt cttcctggag aagccggcat cagcggtgtt
     2041 ggcgtgaggc tggaatgtgc gttgatcacc gaggggcggg atcgcgaacc c
//
LOCUS       DAOQOC010000514         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34885_length_2088_cov_3.488441, whole genome shotgun sequence.
ACCESSION   DAOQOC010000514 DAOQOC010000000
VERSION     DAOQOC010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34885_length_2088_cov_3.488441"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /locus_tag="PL106_08775"
     CDS             <1..2033
                     /locus_tag="PL106_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33201.1"
                     /translation="LPGDGHITGMAARPNGNMVLTGPRTDGTAWLAELDPTGTVLASA
                     HFGEPGDGTVPTRLLLTPDGGVRIAGVVGSAPQRLFSYASAGIGEPGDCSVPDTWPTF
                     QATSLVPYGTLLHDSGTGSFDAPVVAAATNSYCTAATTCSAGGLHLQGTLFHDADGNG
                     AYDSGESCFPNLITELQPDAVYAATNAECAFDLFTAQPGTHVLAPVPLDSWWTISTGS
                     SAYTVEYTGGDSTITGLDFGYIPGLDTTVLTATLVTAPVLCNNYFGNPVQGWLHVLNT
                     GTTLPDVVVALTLDSAIGVVSSEPPVDSVVGPTAYWHLDAPGYYDIASIHMQLQSPFM
                     LDPGDTLHGEGALLLPGTLDTVQVFDWAGMVFCSYDPNDKLVTPMGVGTYGSIPPDTE
                     WLSYVVRFQNTGTYAAADITVQDQLDPLVDPLSLQVLGGSHTLTGLHISGAGLVDFRF
                     DNIQLPDSGADEAGSHGHIAFRVRPRPGLHHLDSIRNNAAIFFDQNAPVITNMVRNTI
                     VNCPEAPWSAMIIAGGEDELWANYDPNGVFFFTYQWLFNGEPVPGATDMMLEATANGS
                     YSVRLTDQYGCVKESEAVEVIVTGMATGAARRLHIYPDPMSEQAVVVLPEGWHGEGPL
                     RILDATGRLVRSIPLVGGPIVTIERGGLVGGQYVLHFVGVDGAVAVARLIVR"
BASE COUNT          367 a          709 c          655 g          357 t
ORIGIN      
        1 tactgcccgg ggacggccac atcaccggca tggcggcacg tccgaacggg aacatggtgc
       61 tcaccggccc gcgcacggac ggcacggcct ggcttgccga actggatccc acggggacgg
      121 tgctcgcctc ggcgcacttc ggcgagccgg gcgatgggac cgtgcccacg cggttgctct
      181 tgacgcccga tggcggcgtg cgcatcgccg gggtcgtggg cagcgcacca cagcgcctgt
      241 tcagctacgc atcggctggt atcggtgaac cgggcgattg ctcggtgccg gacacctggc
      301 ccaccttcca ggccaccagc cttgtgccct acgggacgct cctccacgac tcaggcaccg
      361 gctctttcga cgccccggtg gtggccgcag caaccaacag ctactgcacg gccgccacga
      421 cctgctccgc aggcgggctc caccttcagg gcacgctctt ccacgatgcc gacgggaacg
      481 gtgcctacga cagcggtgag tcctgcttcc cgaacctgat cactgaacta cagcccgatg
      541 ccgtgtatgc ggcaacgaac gccgaatgtg cctttgatct gttcacggcc cagcccggca
      601 cccatgtgct ggcgcccgtg ccattggact cgtggtggac catcagtacg ggcagttccg
      661 cctacaccgt ggagtacacc ggcggcgatt ccaccatcac cggcctcgat ttcgggtaca
      721 tccccggcct ggacaccacc gtgctcaccg ccaccctggt gaccgcgccc gtgctttgca
      781 acaactactt cggcaacccc gtgcaaggct ggttgcacgt gctcaacacc gggaccacgc
      841 ttcccgatgt ggtggtcgca ttgaccctgg acagcgcgat cggcgtggtc tcctccgagc
      901 caccggtgga cagcgtggtg gggccgaccg cgtactggca cctcgacgcc cccgggtact
      961 acgacatcgc cagcatccac atgcagctgc aatcgccgtt catgctggat ccgggcgata
     1021 cgctgcacgg cgaaggagcg ctgcttctgc cgggcacgct cgataccgtc caggtgttcg
     1081 attgggccgg gatggtattc tgctcgtacg acccgaacga caagctcgtg accccgatgg
     1141 gggtcggcac ctatggcagc atcccaccgg acaccgaatg gctctcctac gtggtacgct
     1201 tccagaatac aggtacgtat gccgctgccg acatcacggt acaggatcaa ttggacccgc
     1261 tcgtcgatcc gctctcgctg caggtcctgg gcggctccca cacgctcacc gggctccaca
     1321 tcagcggtgc gggcctggtg gacttccgat tcgacaacat ccagttgccc gacagcggtg
     1381 ccgatgaagc gggcagccac ggccatatcg ccttccgggt acgcccccgc ccaggcctcc
     1441 atcacctgga cagcatccgg aacaacgcgg ccatcttctt cgaccagaac gcgccggtga
     1501 tcaccaacat ggtgcgcaac accatcgtca actgccccga ggcaccctgg tccgccatga
     1561 tcattgcagg aggggaggac gagttatggg ccaactacga tcctaacggg gtcttcttct
     1621 tcacgtacca gtggttgttc aatggggagc ccgttccggg ggccacggac atgatgctgg
     1681 aagcgacggc gaacggcagt tactccgtgc ggctcactga tcaatacggc tgcgtgaagg
     1741 aaagcgaggc cgtggaggtg atcgtcaccg gaatggccac gggtgccgcg aggaggctcc
     1801 atatataccc ggatccgatg agcgaacagg cggtggtggt actgcccgag gggtggcatg
     1861 gggaaggacc cctgcggatc ctggacgcca ccggacgcct ggtgcggagc attccattgg
     1921 tcggaggccc catcgttacc atcgaacgcg gcggcctggt gggcggccag tacgtgctgc
     1981 acttcgttgg cgtcgatggg gcggtcgctg tcgcgcggct catcgtgcgc tgacgacatc
     2041 acagccaagt gcccatgggt ggagccgacc actcaacggg gaacgcta
//
LOCUS       DAOQOC010000515         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_34891_length_2088_cov_3.141171, whole genome shotgun sequence.
ACCESSION   DAOQOC010000515 DAOQOC010000000
VERSION     DAOQOC010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34891_length_2088_cov_3.141171"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PL106_08780"
     CDS             complement(<1..948)
                     /locus_tag="PL106_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HNA33202.1"
                     /translation="MIALLFTACGGPGTSPAAQKREGTDQNVHIMDPHSHARPEEAVI
                     THLSLDLNVDMEARRLKGTAAYAINRGKGDTIRFDTDGLTIHSVKLADGTDAPYTLGD
                     TSFLGRELAVKLPAGADSLIIAYETGPGARALQWLSPGQTADKTKPFLFTQGQAILTR
                     TWIPVQDSPGIRFTYDAKVTVPNDLMAVMSATNPQERNADGVYHFRMDKAIPAYLVAL
                     AVGDIVFKPIDERTGIYAERTMIDKAVWEFADMGKMVSTAETLYGPYRWGRYDVIVLP
                     PSFPFGGMENPMLTFATPTVIAGDRSLTALIAHELAHSWS"
     gene            1142..1215
                     /locus_tag="PL106_08785"
     tRNA            1142..1215
                     /locus_tag="PL106_08785"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1176..1178,aa:Pro,seq:ggg)
     gene            1260..1514
                     /locus_tag="PL106_08790"
     CDS             1260..1514
                     /locus_tag="PL106_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNA33203.1"
                     /translation="MASFYILYSAPLDRYHIGHTTEPTHERLRKHLSLHRGWTARAHD
                     WTVAYIEDHSDKATAYRRELEVKSWKRRDRIEALIRGENR"
     gene            1842..>2088
                     /locus_tag="PL106_08795"
     CDS             1842..>2088
                     /locus_tag="PL106_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA33204.1"
                     /translation="MRTFLLIWALIAAFNGLRAQTPSTVSGYVNDSTSGEALIGASIF
                     IKEIGKGISTNTYGYWSVTLPNGRYTFTVRYIGYATFE"
BASE COUNT          401 a          636 c          629 g          422 t
ORIGIN      
        1 gctccaactg tgcgcgagtt catgggctat aagcgcggtg agcgaacgat cgcccgcgat
       61 caccgtgggc gtggcgaagg tgagcatcgg attctccatg cctccgaagg gaaagctggg
      121 cggcagtacg atcacatcgt agcggcccca gcggtatggc ccgtacaacg tctcggcggt
      181 gcttaccatc ttgcccatat cggcgaattc ccaaacggcc ttgtcgatca tggtgcgttc
      241 cgcatagatc cctgtgcgct catcgatggg tttgaaaaca atgtcgccca cggccagtgc
      301 caccaggtac gcgggtatgg ccttgtccat gcggaaatga tacacgccat cggcattccg
      361 ctcctgcgga ttggtggcgc tcatcaccgc catcaggtca ttcggcacgg tcaccttcgc
      421 atcataggtg aaacggatcc cggggctgtc ctgcacgggg atccaggttc gcgtgaggat
      481 cgcctgacct tgggtgaaga ggaatggctt ggtcttatcg gctgtttgcc cgggcgacag
      541 ccattgaagg gcacgcgctc cagggccggt ctcgtatgcg atgatcaggc tgtccgcccc
      601 ggctggcagc ttcaccgcca gttcgcgccc caggaacgat gtgtcgccga gggtgtaggg
      661 ggcatcggtg ccatcggcca gcttgaccga gtggatggtc agaccatcgg tgtcgaatcg
      721 gatggtgtcg cccttgcccc tgttgatggc atacgccgca gtgcccttca ggcgacgggc
      781 ctccatgtcc acgttcaggt ccagggagag atgggtgatc acggcctcct cgggccgggc
      841 atggctatgg ggatccatga tgtggacgtt ctggtcagtg ccttcccgct tctgtgcggc
      901 aggtgatgtt cccggcccgc cacaagcggt gaaaagcagt gcgatcacgg aagctattga
      961 ccaagaggcg tatcgcatgg gaggtttcgt gccgcacttc accaggactc cggcgttggt
     1021 gcgggcacga aggtaggcgg accggtatgg gtagcgccac tgcctgaagc gcatttggac
     1081 ctgtttgatg gatcccaacc gcttcgcttc ggatcccttc aattacattc gcggcccaat
     1141 tcggggtgta gcgcagcccg gtagcgcact tgcatggggt gcaagtggtc gctggttcga
     1201 atccagtcac cccgactgat cgtgcaggga ggccaattcg gcctccttgc ctttttctga
     1261 tggccagctt ctacatcctg tattccgcac cgctggatcg gtatcacatc ggccatacca
     1321 cggaacccac gcacgaacga ctacgcaagc atttgtccct ccaccgcgga tggacagccc
     1381 gtgcgcatga ctggaccgtt gcgtacatcg aagatcactc cgacaaggcc acagcgtacc
     1441 gtagggaatt ggaggtcaag tcctggaaaa ggcgtgatcg gatcgaagcc ctgatccgtg
     1501 gtgagaatcg ctagcgcagc ccggttgcgc atcccgccac gagggcggga tggtcgctgg
     1561 tccggatcca gtcaccccga cggaatgaaa ggccgtccga catgcacggt cctacgtgct
     1621 cttcacaccg catcaggctc ggaacgacca caacagcaat gatcaccagc cctgtgcgtt
     1681 catgatggat cgccaacgaa ggcggaatgc ttgcctgctc cgatgggcat cgatcatcac
     1741 cacgcatgcg gtacccgaag aagcgaacgg tattctttaa ggatgagcgg cgggagcagt
     1801 gcatgccaca cctctacttt cgcgccgcct tcggcccgta catgcgaacg ttcctcctca
     1861 tctgggccct gatagccgcg ttcaacgggc tccgcgcgca gactccatcc accgtcagcg
     1921 gctatgtgaa cgactccacc agcggcgagg cactcatcgg ggccagcatc ttcatcaagg
     1981 agatcggtaa aggcatttcc acgaacacct atggctattg gtcggtcaca ttgcccaacg
     2041 gtcgttacac cttcacggtg cgctacatcg gctacgccac cttcgagc
//
LOCUS       DAOQOC010000516         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35114_length_2082_cov_3.998027, whole genome shotgun sequence.
ACCESSION   DAOQOC010000516 DAOQOC010000000
VERSION     DAOQOC010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35114_length_2082_cov_3.998027"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="PL106_08800"
     CDS             complement(<1..2067)
                     /locus_tag="PL106_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA33205.1"
                     /translation="MFSHVAGIFLHTSDGPEQGEDMPHTMVQDLHGDLWVSGTTGLQK
                     MTLNGKARADGLPVYSMKKVLRRSAWVSMDRSGTLWASELDHGSFRILPRHDGADRMD
                     TLRADRPMGNNGRGRDLDDLSGLMTVEDTVRGVLYGVHKFGIVRLDPGSTEVRSIVVF
                     AAPYGDMRSSALSTDGKGRLWISTYPGQYLFDPANGRMHLVRPVDPNLTQRAGVVQMN
                     FRDRNGLLWIGTSGYGLLTYDERTERFHTLPGFSCGAMHYGTRGRISIGLADHFLAEF
                     DPGSGTWPMKLPLSVYLRRPELQVFNNAPMFPAQDDAGIYWFNYAGIARYDPATDAIE
                     RLPRDASDPHAREDDHCAPLYLDGDSLIWFGSRTAFGRYDRRMGQYRYHDYPEGVSAN
                     GRNLVTQAIHRSKDGDFWLGTVSGLLHFQPASGTWQRFLNDPGDSTSLAANSVLCITP
                     DPQRPDERLWLGTYGGGMERFDIRTGRCSHISTRQGLPNNVVYAVLPDDAGRLWISTN
                     KGIARYDPRTGRVRTFRASDGLQSDEFNRYAACKLPDGSLCFGGVNGLNHFRPQELEE
                     DSTADPIRITGIRLINHLIDTRDPGSPLRQVPWISTGFSIPHAVNMVTFEFATMEYSA
                     PAEHQYQYRLEGFDADWIMAGTEHSAVYTNLDPGAYTFHVRGRNRDGVWAEHGVTFRL
                     TVLPPWY"
BASE COUNT          401 a          651 c          637 g          393 t
ORIGIN      
        1 gtaccacggg ggcagtacgg tgagcctgaa ggtgacgcca tgttcagccc agacgccatc
       61 gcggttccgg ccgcgcacat ggaatgtgta agcgcccgga tccaagttgg tgtacaccgc
      121 gctgtgctcg gtgcccgcca tgatccaatc cgcatcaaaa ccctccaacc tgtactggta
      181 ctggtgttca gccggggccg agtactccat ggtggcgaat tcgaacgtga ccatgttcac
      241 cgcatgtgga atgctgaatc ccgtgctgat ccaggggacc tgccggagcg gtgagcccgg
      301 atcacgggta tcgatgaggt gattgatgag ccggatgccg gtgatgcgga tggggtcggc
      361 ggtgctgtcc tcttccagtt cctgcggacg gaaatggttc aagccgttca cgccaccgaa
      421 gcacagcgag ccgtcgggta gcttgcacgc ggcgtatcga ttgaactcat cgctctgcag
      481 gccatcgctt gcgcggaagg tccgcacacg accggtgcgc ggatcgtagc gcgcgatgcc
      541 cttattggta ctgatccaca gcctgcccgc atcatcgggc agcacggcat agaccacgtt
      601 gttcggcaac ccctgccttg tgctgatgtg cgaacagcgc ccggtacgga tgtcgaagcg
      661 ttccattcct ccgccatagg tgcccagcca gaggcgctca tcggggcgct gcggatcggg
      721 tgtgatgcac agcacggagt tcgccgcgag cgacgtgctg tcaccgggat cgttcaggaa
      781 ccgctgccag gtgccgctgg caggctggaa gtgcaacagg ccgctgaccg taccgagcca
      841 gaagtcacca tccttgctgc gatggatcgc ctgggtgaca agattgcggc cgttggcgga
      901 taccccttcg ggatagtcgt ggtaacgata ctgtcccatt cgacggtcgt agcgcccgaa
      961 cgcggtgcgt gatccgaacc agatgaggct gtcgccgtca aggtacaacg gggcacagtg
     1021 atcatcctcc cgtgcatgcg ggtccgatgc atcgcgcgga agtcgttcga tcgcatcggt
     1081 ggcgggatca taccgggcga taccggcgta gttgaaccaa tagatgcccg catcatcctg
     1141 cgccgggaac atgggagcgt tgttgaacac ctgcaattcc ggacggcgca ggtacacgct
     1201 cagaggaagc ttcatcggcc atgtaccgct cccgggatcg aactccgcca ggaaatgatc
     1261 ggcgagcccg atgctgatgc ggccgcgcgt gccgtagtgc atggctccac agctgaagcc
     1321 cggcaaggtg tggaagcgct cggtgcgctc gtcgtacgta agcagtccat accccgaagt
     1381 gccgatccac agcagtccgt tgcgatcccg gaagttcatc tgcaccacgc ccgcgcgttg
     1441 ggtgaggttg ggatccaccg gtcgcaccag gtgcatgcgc ccattggcgg ggtcgaacag
     1501 gtactgcccc ggataggtac tgatccacaa ccgtccctta ccatccgtgc tcaaggcgga
     1561 tgagcgcata tccccatagg gcgctgcgaa gaccacgatc gaccgaactt ccgtactgcc
     1621 aggatccaaa cggacgatcc cgaatttgtg cactccgtac agtacacccc gcacggtatc
     1681 ctctaccgtc atcaatccgg agaggtcatc caggtcccgg ccgcggccgt tgttgcccat
     1741 gggccggtcc gcccgtaaag tatccatccg atcggcgcca tcatgccgcg gaagaatgcg
     1801 gaagctccca tggtccagtt cacttgccca caaggtacca ctgcggtcca tggaaaccca
     1861 ggccgaacgg cgaaggacct tcttcatcga atacaccgga agcccgtccg cacgcgcttt
     1921 gccgttcaag gtcatcttct gcagtcccgt ggtgccggat acccagaggt cgccatggag
     1981 gtcctgcacc atggtatgtg gcatatcctc cccctgctcc ggtccatcgc tcgtgtgcag
     2041 gaagatcccc gccacgtggg agaacatgtc aagccctttc tg
//
LOCUS       DAOQOC010000517         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35115_length_2082_cov_3.649729, whole genome shotgun sequence.
ACCESSION   DAOQOC010000517 DAOQOC010000000
VERSION     DAOQOC010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35115_length_2082_cov_3.649729"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="gdhA"
                     /locus_tag="PL106_08805"
     CDS             complement(<1..1343)
                     /gene="gdhA"
                     /locus_tag="PL106_08805"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNA33206.1"
                     /translation="MAKSNKAVDAFMAEVKKRNGNEPEFLQAVHEVAEMVIPFIEANP
                     KYQGKMLLERMVEPERTIIFRVPWVDDKGNTQVNRGYRVEYNSAIGPYKGGIRFHPSV
                     NLSILKFLGFEQTFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFITELWRHIG
                     QFTDVPAGDIGVGGREIGFMFGQDKRLRNEFTGVFTGKGRTWGGSLMRPEATGYGCVY
                     FAEEMMKHNKTSIKGRTVAVSGSGNVAQHAIAKATQLGAKVVTCSDSDGSIYDPAGIN
                     AEKLAFIMELKNVKRGRIEEYARKFKGATYKKGARVWDLVAKCDVALPCATQNELDGN
                     NAKDLVKKGVKYVAEGANMPSTPEAIAVFEAAKVPFAPGKASNAGGVATSGLEMSQNS
                     LRLGWTSEEVDQRLHAIMKNIHAACVKHGTDGKHVNYVKGANIAGFVKVADAMLD"
     gene            complement(1457..>2082)
                     /locus_tag="PL106_08810"
     CDS             complement(1457..>2082)
                     /locus_tag="PL106_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33207.1"
                     /translation="YVGGVPRGDYHTYYPDGRSEEQGSWDLDRNTGTFKRWHPNGQLA
                     QDFLFNEYGVRDGVQKYFHENGQLEVEVNIKEGREDGTLKRYYPNGDLQQVAQFNGGV
                     INEANSKYIKPVHKVESVKPDASAPAAPERRADETTNVAMFRANGTNTLYDKQLRLSQ
                     QGEFKEGRLWNGKYYRYNTSGLLYRIEVYKQGRFAGTAPITDEDKAR"
BASE COUNT          317 a          656 c          643 g          466 t
ORIGIN      
        1 tgatcgagca tggcatcggc caccttgacg aagccggcga tgttggcgcc ctttacgtag
       61 ttcacgtgct taccgtcagt accgtgcttc acacaggcgg catggatgtt cttcatgatg
      121 gcatgcaggc gctggtccac ctcttcggag gtccagccca ggcgcaggct gttctggctc
      181 atctccaggc cgctggtggc cacaccaccg gcgttgctgg ccttgcccgg ggcgaagggc
      241 accttggcgg cctcgaacac ggcgatggcc tcgggcgtgc tgggcatgtt ggcgccttcg
      301 gccacgtact tcacgccctt cttcaccagg tccttcgcgt tgttgccgtc cagttcgttc
      361 tgggtggcgc agggcagcgc cacgtcgcac ttggccacca ggtcccacac acgtgctccc
      421 tttttatagg tagcaccttt gaacttccgg gcgtactcct cgatgcggcc gcgtttcacg
      481 ttcttcaact ccatgatgaa ggcgagcttc tccgcattga taccagcggg atcgtagatg
      541 ctgccgtcgc tgtcggagca ggtgaccact ttggcgccca gctgggtggc cttcgcgatc
      601 gcgtgctggg ccacgttgcc gctgccgctc acggccacgg tcctgccctt gatgctggtc
      661 ttgttgtgct tcatcatctc ctcggcgaag tagacgcagc cgtagccagt ggcctcgggg
      721 cgcatgagcg aaccgcccca ggtgcggccc ttgccggtga atacgccggt gaactcgttg
      781 cgcaggcgct tgtcctggcc gaacatgaag ccgatctcgc ggccgcccac gccgatgtcg
      841 cccgcgggca cgtcggtgaa ctggccgatg tgccgccaca gttcggtgat gaagctctgg
      901 cagaagcgca tcacctcgtt gtcgctcttg cccttggggt cgaaatcgct gccgcccttg
      961 ccaccgccca tgggcagggt ggtcaggctg ttcttgaagg tctgttcgaa gcccaggaac
     1021 ttcaggatgc tgaggttcac gctggggtgg aaacggatac cgcccttgta cgggccgatc
     1081 gcgctgttgt actccacgcg atagccgcgg ttcacttgcg tgttgccctt gtcgtccacc
     1141 cagggaacgc ggaagatgat ggtgcgctcc ggctccacca tgcgctccaa gagcatcttg
     1201 ccctggtatt tggggttcgc ctcgatgaag gggatcacca tctcggccac ttcatgcacg
     1261 gcctggagga attccggttc gttaccgttg cgtttcttca cttcggccat gaaagcgtcc
     1321 acggccttgt tcgactttgc cattggggtg cttcgttttt tgaaagcgag cgcgaaagta
     1381 gatgggcaaa gggcacccgc aaggatgccc gtcatgggtt cccaacaagt tccgatgggt
     1441 tgcccgaaca gcccggctac cgggccttgt cctcgtcggt gatgggcgcc gtaccggcga
     1501 agcggccctg cttgtacacc tcgatgcggt agagcaggcc cgaggtgttg tagcgatagt
     1561 acttcccgtt ccacaggcgg ccctctttga actcaccctg ctgggagaga cggagctgct
     1621 tgtcgtaaag cgtgttggtg ccgttggcgc ggaacatggc cacgttcgtg gtctcatcgg
     1681 cccggcgctc cggcgcggcg ggagcactgg cgtccggctt gacagactcc accttgtgca
     1741 ccggcttgat gtacttgctg ttggcctcgt tgatcacgcc cccgttgaac tgggctacct
     1801 gctgaaggtc cccgttgggg tagtagcgct tcaaggtgcc gtcctcacgg ccctccttga
     1861 tgttcacctc cacctccaac tgtccgttct cgtggaaata cttctgcacg ccatcgcgca
     1921 cgccatactc attgaacagg aaatcctgag cgagctggcc gttggggtgc cagcgcttga
     1981 aggtgcccgt gttgcggtcc aggtcccatg agccctgctc ctcggaccgt ccgtccggat
     2041 agtaggtgtg gtaatcgccg cgaggaaccc cgcccacgta gg
//
LOCUS       DAOQOC010000518         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35213_length_2079_cov_2.961957, whole genome shotgun sequence.
ACCESSION   DAOQOC010000518 DAOQOC010000000
VERSION     DAOQOC010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35213_length_2079_cov_2.961957"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PL106_08815"
     CDS             <1..273
                     /locus_tag="PL106_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33208.1"
                     /translation="QPYPLLEARVYIMYGDHDFPDDDVRTGPDGVFVFSWLRKGDYRV
                     YVVGECRDDCETGCSGEQRITLISTTVDGKSGVTDVGTLSVKNYSN"
     gene            273..1061
                     /locus_tag="PL106_08820"
     CDS             273..1061
                     /locus_tag="PL106_08820"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNA33209.1"
                     /translation="MHPLARIAVLGAVLSLASNAMAQESPMHRPVQDGQLWISGGFEL
                     RFDKEAQKRIKKAKEGRETGKLTGAELERAEKRAAEAERSFKNRFRITGEVDHRRSEN
                     MTRSNLFYTVLGVRYRFHEYARLTLEHRYNIRPSGRNTHRINVQADADRNFGRFNAGY
                     RLAYQHEFTTPDKYRDIVRNRLQLSFRSKRFPVDPYISAESFTAFHHTGNQLIGMRYG
                     IGAEWKLSKDHVLDLSVRHHREHGQPGLKYRWIFNVGYEFKWRR"
     gene            1070..1726
                     /locus_tag="PL106_08825"
     CDS             1070..1726
                     /locus_tag="PL106_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNA33210.1"
                     /translation="MMRPLLLRALLFLLVFAIGVGIAYNLVKPSDELPIYTPGELDPR
                     LVDSSLRRSFDEHRILDFSLTDQSGRKVTGADVEGKVVLADFFFTTCGSICPRMSAQL
                     ERVQAAYRNEARFVILSHTVTPEIDSVPVLSAYAELHGADPERWRFLTGDRRQIYNLA
                     RKSWFAVKDSGDGGPDDFVHTENLVLADTLGRLRGFYDGTDPKDVDRAIGDIRKLLGR
                     "
     gene            complement(1777..>2079)
                     /locus_tag="PL106_08830"
     CDS             complement(1777..>2079)
                     /locus_tag="PL106_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA33211.1"
                     /translation="MRVGIHSGTVVAGIVGDTKFQYDVWGDAVNIAARMETAGEEGRV
                     NISAATYALVKDDPALSFVERGDVNTKGKGVLAMFFVSRRIAGEQVEQARYAGADG"
BASE COUNT          408 a          653 c          649 g          369 t
ORIGIN      
        1 cagccctacc cgctgctgga agcacgggtg tacatcatgt acggcgatca tgacttcccc
       61 gacgacgatg tgcgcaccgg gcccgatggg gtcttcgtgt tcagctggct gcgcaagggg
      121 gactaccgcg tgtacgtggt gggcgaatgc cgcgatgact gcgagaccgg ttgttcgggc
      181 gaacagcgga tcacgctcat cagcacaacg gtggatggca agagcggtgt gaccgacgtg
      241 ggcacgctca gcgtgaagaa ttattcgaat tgatgcatcc gctggcgcgc atcgccgtgc
      301 tgggggccgt gctgtcgctg gcctcaaatg ccatggcaca ggagtctccg atgcaccggc
      361 ccgtgcagga tgggcaactg tggatcagcg gagggtttga gctgcggttt gacaaggagg
      421 cccagaagcg gatcaagaag gccaaggagg ggcgggagac cggcaagctc accggcgccg
      481 agttggaacg agcggaaaag cgcgcggccg aagcggagcg gtcgttcaag aatcggttcc
      541 gcatcaccgg cgaggtggac catcggcgca gcgagaacat gacccggagc aacctcttct
      601 acaccgttct cggggtgcgc taccgcttcc atgaatacgc acgcctcacc ctggagcaca
      661 ggtacaatat ccgtccgagc ggccgcaaca cgcaccgcat caatgtgcag gcggatgccg
      721 accggaattt cggccgcttc aacgccggct accggttggc gtaccagcac gaattcacca
      781 cgcccgacaa gtaccgtgac atcgtccgca acaggctcca gttgtcgttc cgcagcaagc
      841 ggttcccggt ggacccgtac atctccgcgg agagcttcac ggccttccat cacaccggga
      901 accagctcat cggcatgcgg tacggcatcg gcgcggaatg gaagctcagc aaggaccacg
      961 tgctggacct gagcgtgcgg catcatcgcg aacatggcca gcccggactg aaatacaggt
     1021 ggatcttcaa tgtcgggtat gaattcaagt ggcggaggtg acaccgttca tgatgagacc
     1081 gctccttctc cgcgcgctcc tgttcctgct cgtcttcgcc atcggggtgg ggatcgctta
     1141 caacctcgtg aaaccgagcg atgaattgcc catctacacg cccggcgaac tcgatccgcg
     1201 gctggtggac agttcgttgc ggcgatcatt cgacgagcac cgcatcctgg atttcagcct
     1261 caccgaccag tcgggtcgca aggtgaccgg agccgatgtg gaaggcaagg tggtgctggc
     1321 cgatttcttc ttcaccacgt gtggcagcat ctgtcccagg atgagcgccc agttggagcg
     1381 cgtgcaggca gcgtacagga atgaagcgcg tttcgtgatc ctctcccaca cggtgacccc
     1441 cgagatcgac agcgttcccg tgctctccgc ctacgccgaa ctgcatggcg cggatcccga
     1501 gcgctggcgc ttcctcacgg gcgaccgccg ccagatctac aacctggcgc gcaagagctg
     1561 gttcgcggta aaggacagcg gcgatggcgg ccccgacgac ttcgtccata ccgagaacct
     1621 cgtattggct gacacgctgg ggcgtcttcg gggattttat gacggcaccg accccaagga
     1681 tgtggaccgt gcgatcgggg acatccgcaa gctcttgggg aggtagggcg ccgggctggc
     1741 cccatcgcat caggcgatgg cgcaaggcca tgcgcatcag ccatccgcac cggcgtaccg
     1801 ggcttgttcc acctgttcgc ccgcgatcct gcgactgacg aagaacatgg ccagaacacc
     1861 ctttcccttg gtattcacat cgccccgttc gacgaaggag agagcaggat catccttcac
     1921 cagcgcatag gtcgcggcac tgatattcac tcgcccctcc tcgcctgcgg tctccatgcg
     1981 ggcggcgatg ttcacggcat cgccccagac gtcgtactgg aacttcgtgt ccccaacgat
     2041 accggccacc accgtgccgg aatggatgcc gaccctcat
//
LOCUS       DAOQOC010000519         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35314_length_2076_cov_3.197427, whole genome shotgun sequence.
ACCESSION   DAOQOC010000519 DAOQOC010000000
VERSION     DAOQOC010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35314_length_2076_cov_3.197427"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1470)
                     /locus_tag="PL106_08835"
     CDS             complement(121..1470)
                     /locus_tag="PL106_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA33212.1"
                     /translation="MSRITIVGGGLVGGLLAVFLAKRGHAVKVFDRRGDPRRTDVYAG
                     RSINLVVSHRGWTALRAAGVEEAVRAITVPVYARMTHDRDGRLNRVPYSIDNRAIFSV
                     SRGELNRRLLSEAEKLPNVELHFDHKCVDVDLHTGECVFEHGGERVLEEADVVFGSDG
                     AFSAVRQKMMLGRFTYSQEYIEHDYKEIAFPANADGTPKMDPHCLHIWPRRFFMMMGL
                     ANQDGGFTGTLFMPNTHTDQAPGFDVVRTGEQARRFFGEHFPDAIPIAPDIVDQYLRN
                     PQSSLVIIRCAPWQVNGRVALIGDAAHAIVPFYGEGMNAGYEDCKVLNDLLNEYGDDN
                     WAEVLTRYAAERKPNGDAIADLSLRNFVEMRDLVADPKFLLRKKIEGHLQQRHPDKWL
                     PLYSQVKFSDIAYKDAWKEGLRHDRIMEEVLAIPGIEEKWESAEVVKRVMGMLEATV"
     gene            complement(1467..>2076)
                     /locus_tag="PL106_08840"
     CDS             complement(1467..>2076)
                     /locus_tag="PL106_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNA33213.1"
                     /translation="HNWDADFAVWCSYKYLNSGPGGPAGAFVHERHAAQPDLPRFAGW
                     WGHDKNERFLMEPEFKPMASAEAWQLSNAPVFSMAVHRAALDLFDRAGMDALRAKSEE
                     LTGYLAFVLKTISAAHGDPFTIITPDDPAQRGCQLSLLVRENGRALFDTLTRNGVVVD
                     WREPDPSTSLRAGVIRMAPVPLYNSFEDVFRFGGILDNALRS"
BASE COUNT          382 a          703 c          600 g          391 t
ORIGIN      
        1 tcgtgactca cgaggtggac ccttatgaac gtgcaggagc agcttgtggc gcctcatggt
       61 cacgaccatc tcgaacgcac atcacggttg cggagaccct gaggaagtgt agttcacgca
      121 tcacacagtc gcctccaaca tccccatcac cctcttcacc acctcggcac tctcccactt
      181 ctcctcgatc ccgggtatgg ccagcacctc ctccatgatc cggtcgtgcc gcagcccttc
      241 cttccacgca tccttgtaag cgatgtcgct gaacttcacc tggctgtaca ggggcaacca
      301 cttgtcgggg tggcgctgct gcaggtggcc ctcgatcttc ttgcgcagca ggaacttcgg
      361 atcggccacc aggtcgcgca tctccacgaa gttgcgcaag ctcaggtcgg cgatggcatc
      421 gccgttgggt ttgcgctcgg cggcgtaacg cgtcagcacc tcggcccagt tgtcgtcgcc
      481 gtactcgttc agcagatcgt tcagcacctt gcagtcctcg tagccggcgt tcatgccctc
      541 accatagaac gggacgatgg cgtgcgccgc atcgccgatc aaggccaccc tcccgttgac
      601 ctgccagggt gcgcaacgga tgatcacgag cgaactctgc gggttccgca gatactggtc
      661 cacgatgtcg ggcgcgatgg ggatggcgtc ggggaagtgt tcgccgaaga accgccgcgc
      721 ctgctccccg gtccgcacga catcgaagcc gggcgcctgg tcggtgtgcg tgttgggcat
      781 gaacaatgta cccgtgaatc cgccgtcctg gttggccagg cccatcatca tgaagaacct
      841 gcgcggccag atgtgcaggc aatgcggatc catcttcggg gtgccatcgg cgttggccgg
      901 aaaggcgatc tccttgtagt cgtgctcgat gtactcctga ctgtacgtga agcggcccag
      961 catcatcttc tggcgtacgg cgctgaaggc accgtcgctg ccaaagacca catcggcctc
     1021 ctccagcacc cgttcgcccc cgtgttcaaa tacacattca ccggtatgaa ggtccacatc
     1081 gacgcacttg tggtcgaagt gcagctccac attcggcagt ttctcggctt cgctgagcag
     1141 acgccggttc aattcgccgc gcgataccga gaagatcgcc cggttgtcga tgctgtacgg
     1201 aacacggttg agcctcccgt cgcgatcgtg ggtcatgcgc gcgtacaccg gcaccgtgat
     1261 ggcccgcacg gcctcctcca ccccggcggc gcgcaaggcc gtccaacccc tgtgcgagac
     1321 caccaggttg atactgcgtc ccgcgtacac atcggtcctg cgcggatcgc cacgccgatc
     1381 gaacaccttc accgcatgcc cgcgtttcgc caggaacacc gcgagaaggc cgcccaccaa
     1441 cccaccgccg acgatcgtga tcctgctcat gaacgcagcg cattgtccag gatgccgccg
     1501 aaacggaaaa catcctcaaa actgttgtat agcggcaccg gtgccatgcg gatgacgccc
     1561 gccctgagcg atgtcgacgg atcgggttcg cgccagtcga ccaccacacc attcctcgtg
     1621 agcgtatcga acagggcgcg cccgttctcg cggacgagca aactgagctg gcagccgcgc
     1681 tgtgccggat catcgggcgt gatgatggtg aagggatccc cgtgcgcggc agaaatggtc
     1741 ttgagcacga acgcgaggta gcccgtgagc tcttcgctct tcgcgcggag cgcatccatg
     1801 cccgcccgat cgaagagatc cagcgccgca cgatgcacgg ccatgctgaa gaccggtgcg
     1861 ttgctcagct gccacgcctc ggcactggcc atgggcttga actccggctc catcaggaag
     1921 cgctcattct tgtcgtggcc ccaccagccc gcaaaacgcg gcaagtctgg ctgcgctgcg
     1981 tgtcgctcgt gtacaaaagc gcccgctggc ccccccggac ccgaattgag gtatttatag
     2041 ctgcaccata ccgcaaaatc tgcatcccag ttgtgg
//
LOCUS       DAOQOC010000520         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35335_length_2075_cov_4.026733, whole genome shotgun sequence.
ACCESSION   DAOQOC010000520 DAOQOC010000000
VERSION     DAOQOC010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35335_length_2075_cov_4.026733"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>2075)
                     /locus_tag="PL106_08845"
     CDS             complement(297..>2075)
                     /locus_tag="PL106_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33214.1"
                     /translation="VTPDAFQPTTDGNDFYLAVFDIDMNGLFYATYFGGSQSHEHVDG
                     GTSRFDRRGRVYEAVCAGCGGHDDFPSTPGAWSPTNNSTNCNLGVFKFDFNFPIVTAD
                     FDAVVNCLPAPVTFTDHSYGATGHTWTFGDGGSSTDPAPEHTYDQPGVYTVTLVSFNS
                     ASCNLSDTMHRQVVVLGNAAYALPDTFACTGTSVQIGLLPVDNPSVTFAWSPSQGLSN
                     TSVPNPVATPAGTITYTLLMSNGACTDTITQTITVPDTQLDAGPDILRCGPDASATLT
                     ANGFGTTDQFQWSSNNSFTDTLNAVLADSTVVVQLTGTTLFYVRAPNDPCGAYDSVQV
                     VVQLAAPLLTGDSLICAEDVARLHVIGADPGSSFTWSPNADIDTGQGTANATVSPPAQ
                     QTFSVDVVSPAGCTWTGSITVTVSPLFGNTMGASVDQPIVLSGTTVQLSAMPDSGVTY
                     AWTPPNEVSDPSIANPTACITGTSTFIVTITDGICTRNDSVTVTVHEMNCAEPDIYIP
                     DAFTPNGDGNNDVLLVRGRFITTMQLKVFDRWGELVFETTDQDVGWDGTYKGKPVDPA
                     VFVYWLKAVCADGQSFLKKGNITVIR"
BASE COUNT          352 a          593 c          717 g          413 t
ORIGIN      
        1 ccgtgacgct gcgccactgc tggcggtaca cggcggccgc ccggtactgc ccatcgaaca
       61 accccgtgtt ggccgggttc gtggcgtacg gtgtgttgaa gaactgcgtg aaatggatgt
      121 cctgtgcgct catgcacaac ggcaccgatg tacaggacca ggcgatcagt gccctgaccc
      181 acaggggggg gcggaacgtg gtgatccccg gtgccccagg acaccgttca ccatcggccg
      241 aggaccgttt tccgttcacc atcgatcttc acgatccgcc atccggcaag tgcctttcat
      301 cggatcacgg tgatatttcc tttcttgagg aacgactggc cgtcggcgca cacggccttc
      361 agccaataca cgaagaccgc cgggtccacg ggcttgcctt tgtatgtgcc gtcccaaccg
      421 acatcctggt ccgtggtttc gaagacgagt tcgccccagc ggtcgaagac cttgagttgc
      481 atggtggtga tgaagcggcc ccgtacgagc agcacgtcgt tgttgccgtc gccgttgggt
      541 gtgaaggcgt cagggatgta aatgtcgggc tcggcgcagt tcatctcgtg gacggtcacc
      601 gtcaccgagt cgttccgggt gcagatgccg tcggtgatgg tcacgatgaa cgtgctggtc
      661 cccgtgatgc acgcggtcgg gttggcgatg gaggggtcgc tcacctcgtt tggcggggtc
      721 caggcgtacg tgacaccgga atcgggcatc gcactcaatt gtaccgttgt tccggaaagt
      781 acgatcggct ggtccacact ggcgcccatg gtattcccga atagcgggga aacggtcacg
      841 gtgatgctcc cggtccaggt gcagccagcg ggcgatacca catcaacgga gaaggtctgc
      901 tgggcgggtg ggctcacggt ggcgttggcc gttccctgtc ccgtgtcgat atccgcattc
      961 ggcgaccagg tgaagctgct gcctggatca gccccgatca catgcaaccg agccacgtcc
     1021 tccgcacaga tgagcgaatc gccggtgagc aggggtgcgg ccagttgcac gaccacctgc
     1081 acgctgtcgt acgcaccgca aggatcgttg ggcgcccgca cgtagaagag cgtggtgccc
     1141 gtgagttgaa cgaccaccgt gctgtccgcg agcaccgcgt tgagcgtatc ggtgaagctg
     1201 ttgttgctgc tccactggaa ctggtcggtg gtgccgaagc cgttagcggt gagcgtggcg
     1261 ctggcgtcgg gtccgcagcg caggatatcc gggccggcat caagttgcgt atccggcacg
     1321 gtgatggtct gcgtgatggt gtctgtgcag gcaccgttgc tcatcagcag ggtgtacgtg
     1381 atcgtgcccg cgggtgtggc cacggggttg ggtacggagg tgttgctgag tccctgcgat
     1441 gggctccacg cgaaggtgac ggacgggtta tcgacgggca gcagcccgat ctgcacgctg
     1501 gtgcctgtgc aggcgaaggt gtcgggcagg gcgtacgcgg cattcccgag gaccaccacc
     1561 tggcggtgca tggtatcgct caggttgcat gaggcactgt tgaacgatac gagtgttacg
     1621 gtatacacgc cgggctggtc gtacgtgtgc tccggggcgg gatccgtgga actcccgcca
     1681 tcgccgaacg tccacgtgtg tccggtggca ccgtagctgt ggtcggtgaa ggtcacgggg
     1741 gccggaaggc agttgaccac tgcatcgaag tcggccgtga cgatggggaa gttgaagtcg
     1801 aacttgaaaa caccgaggtt gcaattggtg ctgttgttgg tggggctcca ggcgcccggt
     1861 gtactcggaa aatcatcgtg gccgccgcag ccggcgcaca cggcctcgta cacgcgcccg
     1921 cgccggtcga atcggctggt gccaccgtcc acgtgctcgt ggctctggct tcctccgaaa
     1981 tacgtggcat agaacagacc gttcatgtcg atgtcgaaga ccgcaaggta gaagtcgttg
     2041 ccgtccgtgg tgggttggaa ggcgtcgggt gtgac
//
LOCUS       DAOQOC010000521         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35369_length_2074_cov_3.698366, whole genome shotgun sequence.
ACCESSION   DAOQOC010000521 DAOQOC010000000
VERSION     DAOQOC010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35369_length_2074_cov_3.698366"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PL106_08850"
     CDS             complement(<1..660)
                     /locus_tag="PL106_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNA33215.1"
                     /translation="MAKKQASGKKGKKTRARAGTNWLKVALVVFAVLLIGFFGFMQAV
                     RAGLFGRLPTEEELRSIRHEQATLVLAADGSLIGKLFAEDRTNVRFEDLPQHLIDALV
                     STEDARFFSHQGVDGRSYVRVFFRTLLGGDASGGGGSTISQQTIKNLYGRQNHGLLTV
                     PVNKMKEALVAQRLERVLSKNDVLTLYFNSVPFGENVFGIEAAAQRFFNTTTRRLTVE
                     ES"
     gene            complement(718..1980)
                     /gene="hflX"
                     /locus_tag="PL106_08855"
     CDS             complement(718..1980)
                     /gene="hflX"
                     /locus_tag="PL106_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNA33216.1"
                     /translation="MIDVRISGAQGETAVLVGLITAPDRDQPGQSIEQVTEYLDELEF
                     LATTADIRTLKRFTQKLHKPDGRTYVGSGKLMEIKIWMEEHGAEMAIFDDELTPAQQR
                     NLEKDLGKPVLDRPRLILDIFARRARTSHARTQVELARYEYMLPRLTKLWTHLERQRG
                     GTGTRGGAGEKEIETDRRLVRDRIALLKGQLRDIDKQMATQRGNRGKLVRVALVGYTN
                     VGKSTLMNLLSKSEVFAENKLFATLDTTVRKVVLGNLPFLLSDTVGFIRKLPHQLIES
                     FKSTLDETREADLLLHVVDISHHAFEEQYMTVKQTLSGIGAGDKPVITVFNKIDAYRP
                     DAYDPHSLSRDGGDLSPKRSGQFTLEELTETWMARMADEECVFISAAQRANIDALRKL
                     LYQRVKAIHVARYPYESDLLFKDEYLEP"
BASE COUNT          330 a          638 c          659 g          447 t
ORIGIN      
        1 actctcctcc acggtgagtc ggcgcgtggt ggtgttgaag aaccgttgtg ccgcggcctc
       61 gatgccgaag acgttctccc cgaagggcac cgagttgaag tagagggtga gcacgtcgtt
      121 cttgctgagc acgcgctcga gtcgctgggc gaccagggcc tccttcatct tgttcacggg
      181 cacggtgagc aggccgtggt tctggcgtcc gtacaagttc ttgatcgttt gctgactgat
      241 ggtgcttcct ccgccaccac tggcatcgcc accgagcagg gtccggaaaa atacccgcac
      301 ataacttctt ccatccacgc cctgatggct gaaaaagcgc gcgtcctcgg tgctgacgag
      361 cgcatcgatg aggtgttgcg gcaagtcctc gaagcgcacg ttggtgcggt cctcagcgaa
      421 cagcttgccg atgagtgagc catcggccgc gagcaccagg gtggcctgtt cgtggcggat
      481 ggatcgcagt tcctcttcgg tgggcagcct gccgaaaagg cccgctcgca cggcctgcat
      541 gaagccgaag aagccgatga gcagcaccgc gaatacgacc agcgcgacct tcaaccaatt
      601 ggtccccgcc ctggccctgg tcttcttccc tttcttcccg gacgcttgtt ttttggccat
      661 tccttcagcg gtaacgcggg cgaacggccg tgtatgcgcg ggccttgccg gggcttctca
      721 aggctccagg tactcgtcct tgaacagcag gtcgctctcg tacggatagc gcgccacatg
      781 gatggccttc acgcgctgat acaacagctt gcgcagcgca tcgatgttgg cgcgctgggc
      841 ggcgctgatg aagacacatt cctcgtcggc catgcgcgcc atccaagttt cggtgagttc
      901 ctccagggtg aactggccgg agcgcttggg cgacaggtcc ccgccgtcgc gggagaggct
      961 atgcgggtcg tacgcgtcgg gtcggtaggc gtcgatcttg ttgaacacgg tgatcacggg
     1021 cttgtcccct gcaccgatcc cgctgagcgt ctgcttcacg gtcatgtatt gttcctcgaa
     1081 ggcatgatgg ctgatgtcca ccacatggag cagcagatcg gcctcgcgcg tttcatccag
     1141 tgtgctcttg aagctctcga tcagctggtg cggcagcttg cggatgaagc ccaccgtatc
     1201 gctcagcagg aatgggaggt tgcccagcac cactttgcgc accgtggtgt cgagcgtggc
     1261 gaagagcttg ttctccgcga agacctcgct cttgctcagc aggttcatga gcgtgctttt
     1321 gcccacgttg gtgtacccca cgagcgcaac acggacaagt ttgccccggt tgccgcgctg
     1381 ggtggccatt tgcttgtcga tatcgcgcaa ctggcctttg agcagcgcga tgcggtcgcg
     1441 caccagtcgg cggtcggtct cgatctcctt ctcgcccgca ccaccgcgag ttccggtacc
     1501 cccgcgctgg cgctccaaat gggtccagag cttggtgagt cgcggcagca tgtactcgta
     1561 ccgcgccagt tccacttggg tgcgggcgtg ggatgtgcgg gcgcgccggg cgaagatgtc
     1621 caggatcagg cggggacggt ccaataccgg tttgcccaga tccttctcca ggttgcgctg
     1681 ctgggcgggc gtgagttcat catcgaaaat ggccatctcc gcgccgtgct cctccatcca
     1741 gatcttgatc tccatcaact tgccgctgcc tacataggtg cggccatcgg gcttgtggag
     1801 tttctgggtg aagcgtttca gcgttcggat gtcggcggtg gtggccagga actccaactc
     1861 gtcgaggtac tcggtcacct gctcgatgga ctgcccgggc tggtcgcgat cgggcgcggt
     1921 gatgagtccg acgagcaccg ccgtttcacc ttgtgcgcca ctgatcctga cgtcgatcat
     1981 cgggcgggct tcgtacgcag ggtgagcaca tggtcggtca cggccgcgat ctccttcttg
     2041 gccttgttgc ccatcaccca ggcgaacggg ccgg
//
LOCUS       DAOQOC010000522         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35385_length_2074_cov_2.142150, whole genome shotgun sequence.
ACCESSION   DAOQOC010000522 DAOQOC010000000
VERSION     DAOQOC010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35385_length_2074_cov_2.142150"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..380)
                     /locus_tag="PL106_08860"
     CDS             complement(132..380)
                     /locus_tag="PL106_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33217.1"
                     /translation="MEQGLEERMLRIKEYLVGIWLFREPLRTPRWCATFVYRGHYYDV
                     SGKHSPLSAVKAVEQRVKDLEKAHAAQLRKMAAKKQRK"
     gene            complement(416..1132)
                     /locus_tag="PL106_08865"
     CDS             complement(416..1132)
                     /locus_tag="PL106_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33218.1"
                     /translation="MKWTLLSLLLPIWVSGQSITYQWLNEPSCASLLSCDTGCTACNS
                     AVNTQGQFSGTDVAWLGVDVCPHPFALGDNTLFTYGWPAIPNEEHAVLVSGIAFSPVR
                     IDSLVIRHRTGPDGPQRLRVRFGVNETMPATEIADAAVQTEFARTVITDLGTVEATPQ
                     MVYGFFNVVLQPYMGEGGPWELDEIRIVGTPVTMTAIEEVAGIPRSGKAQRYDAAGRM
                     EPSRPDQLLYIDGTRRVVLR"
     gene            complement(1640..1864)
                     /locus_tag="PL106_08870"
     CDS             complement(1640..1864)
                     /locus_tag="PL106_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33219.1"
                     /translation="MPVADGMVERMYREGKAFVLERTITENGHSVVYKRVRHAYATFY
                     FEDGQAVDERVWNARFYGVRMEEASPDDAR"
BASE COUNT          370 a          638 c          645 g          421 t
ORIGIN      
        1 ccggtttctt gaactggatc tcgttcgtgc cgggtcccat cagtgcttca aaggctggaa
       61 cgatcgggca gggccaaggc gggcgagcgg tgcaagtcct ttccgatcaa cgcttctttc
      121 acgatccggg atcatttgcg ctgcttcttc gcggccattt tccggagctg ggcagcgtgc
      181 gccttttcca ggtccttcac acgctgttcc acggctttca ccgcactgag cgggctgtgc
      241 ttgccactca catcatagta gtggccacgg tacacgaagg tggcgcacca gcgcggggtg
      301 cgcaggggtt cgcggaagag ccagatgccc acgaggtatt ccttgatgcg gagcatgcgc
      361 tcctcgagtc cttgttccat gcgttattcg ttgcgccaaa gatgggcgca gccgatcagc
      421 gcaacaccac cctgcgcgtg ccgtcgatat agagcagttg atcaggacgt gatggctcca
      481 tgcgtccggc agcatcgtag cgctgcgctt ttccggatcg tgggataccc gcgacctctt
      541 cgatcgcggt catcgtgacc ggtgttccaa cgatgcggat ctcgtccagt tcccacggac
      601 caccttcgcc catgtacggt tgcagcacca cgttgaagaa gccgtacacc atttgcggcg
      661 tggcctccac cgtgccgagg tccgtgatca cggtccgtgc gaattccgtt tgcacggcag
      721 cgtcggcgat ctctgtggca ggcatggtct cgttcacgcc gaagcgcaca cgcaagcgct
      781 gcgggccgtc gggtccggtg cggtggcgga tcaccagact gtcgatgcgc accgggctga
      841 acgcgatgcc ggagaccagc accgcatgct cctcgttcgg gatcgcgggc cagccgtagg
      901 tgaacagcgt gttgtcgccc aatgcgaagg gatgcgggca aacatccacg cccagccagg
      961 ccacatcggt gccgctgaac tgcccctgcg tgttgaccgc cgaattgcag gcggtacagc
     1021 ccgtatcgca actcaacaac gaagcgcagg agggctcgtt gagccactgg taggtgatgg
     1081 attggcccga gacccatatg ggcagcagca gggagagaag tgtccacttc atggtcgtgc
     1141 cgatcagaat gagtggccgc ttgaacggcg ctgtgggcgg aatgttccgg gataggcggc
     1201 accttattgg accaacgtgc cgccgactgc ccacagccag tagcatgtcc gtcaaatacc
     1261 cggatcaggt cagttatggg acggatggaa agatccgcac ggagcggaca tgggacacct
     1321 ccgggggcga acgttcatgg gctcgggcgt ggaccttcgc acgcttgcgc gcactgacca
     1381 atagacctcc gtctcaattg cccggaggaa tgaaaaagac gtgtcagggt gagcctgccg
     1441 aaccggtcct tgcttcgccg tagcggctac gcaaaggcgc ggtcgacgga ctcaccgtga
     1501 cacgctaatg gaattacgac ggaggtctaa tgaacagcca agtccttgcc ttcagactcc
     1561 gtatggatac gctgcgcggc cttcgcgctc ctcgcattcg ctgaccaacg cttcagatgg
     1621 ggatcacggg cactccctcc taccgcgcgt catccggcga tgcttcctcc atcctcaccc
     1681 cgtagaacct ggcgttccac acgcgttcgt ccacagcctg gccgtcctcg aagtagaagg
     1741 tggcgtacgc atggcgcacc cgtttgtaca ccacggaatg cccgttctcc gttatcgtgc
     1801 gttcgagcac gaatgccttg ccctcccgat acatccgctc caccatgcca tccgccacag
     1861 gcatgggttg cgggatcaca tctggagacg tgttccttgg ggccggttcc gcgctgggtc
     1921 gtggtgcggg cggttccttg gtcggttcag gtagatgtat ttgtaccggt actggtacat
     1981 ctatcaccgg ttcgggagcc ggtgcttcga tgcggggttc ctcgggcccg gatgggtcgg
     2041 gccgctgttc cggcccaggg ttcagaacta cggg
//
LOCUS       DAOQOC010000523         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35399_length_2073_cov_4.087215, whole genome shotgun sequence.
ACCESSION   DAOQOC010000523 DAOQOC010000000
VERSION     DAOQOC010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35399_length_2073_cov_4.087215"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..693
                     /locus_tag="PL106_08875"
     CDS             322..693
                     /locus_tag="PL106_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33220.1"
                     /translation="MRTILTTLALAVGMSLMAQDANAGKDKKTPEERAAKRTEQMTKD
                     LGLSSEQVAKVNTINLNFARYSSDVEAAGKGADVEGRTKALKAKRDAELKAVLTPEQY
                     TKYEEIRAKKQADKKSKDAGN"
     gene            913..>2073
                     /locus_tag="PL106_08880"
     CDS             913..>2073
                     /locus_tag="PL106_08880"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNA33221.1"
                     /translation="MSDKAKIILDGKEYELPIFTGSEGEKAIDISKLRDQSGYITLDL
                     GYKNTGATTSAITFLDGEQGILRYRGYPIEQLAEQSTFIEVAYLLLNGVLPNKKELDD
                     FENQIRYHSLVHESMKHFFEFFPSNAHPMGILSAMVNSLSTFYPKSQIPHRSESERMR
                     TIYRLIAKMPTLAAISYKNNQGHPYMYPDNKLGYVENFLYMMYALPTESYKADPVVVD
                     ALNKLLILHADHEQNCSASTVRMVGSSQANLYSAVSAGIAALWGPLHGGANQEVIEML
                     ETIRNDGGDIRKWVDKAKDKNDPFRLFGFGHRVYKNFDPRARIIKSACDRVLAKIGRK
                     DPLLDIARKLEELALKDPYFVERKLYPNVDFYSGIIMRAIGIPVNMFTVMFSM"
BASE COUNT          482 a          648 c          620 g          323 t
ORIGIN      
        1 aggctggcgg cggcggcgag ggtgcggaag gtgttcatgg cagcggatgt tttcggggtg
       61 cttgtcgttc ttgacgggtc aaaagtcccc cgcccacccc cggttcccca gcgaggatga
      121 gcaggtggcg gaagcgggtg cgcaggtggc ggagcaccac cctgccggac ccgtccaggc
      181 gcaaaaccgg gctgcccgcc cccggcaaac ccccgaggca ggccgtgcgt cttaccaccg
      241 gtcccggttc atttggcgaa gcggcgactt ggcccgacat ttgggtcctc accacactga
      301 acccccaaca caacagagca gatgagaacg atcctaacca cgctggcgct ggccgtcggc
      361 atgagcctga tggcgcagga tgccaacgcg ggcaaagaca agaagacccc cgaggagcgt
      421 gccgccaaac gcaccgagca gatgaccaag gacctgggcc tttccagcga gcaggtggcc
      481 aaggtgaaca ccatcaacct gaacttcgca cgctacagtt ccgatgtgga ggcggccggc
      541 aagggtgccg acgtggaagg ccgcaccaag gcgctcaagg ccaagcgcga tgctgaactc
      601 aaggcggtac tcacgccgga acaatacacc aagtacgagg agatccgcgc caagaaacag
      661 gcggacaaga agagcaagga cgcgggcaac tgatcccgga ccgcgcccac gaagggcccc
      721 ttcacctctt gatccccgtc accaggacgg gggcggagag gcggaggggc ttttttttcg
      781 ggtcggtacg gcctggatac tgacaacgtt gacaaaggag gagaaacacc gccactcggg
      841 aactttgacc ctgatcggga cgttacattc gcgcaccgga aagaaaaccc atccatcaca
      901 gcgcagctac cgatgagcga caaggcgaag atcatcctgg acggcaagga atacgaactg
      961 cccatcttca caggtagcga gggcgagaag gccatcgata tcagcaaact gcgcgatcag
     1021 agcgggtaca tcacgctcga cctcggatac aagaacaccg gcgccaccac cagtgccatc
     1081 accttccttg atggggagca gggcatcctg cggtaccgcg gttatcccat cgagcagctc
     1141 gccgagcaga gcaccttcat cgaagtggcc tacctgctcc tcaacggggt gctgcccaac
     1201 aagaaggaac tcgacgactt cgagaaccag atccgttacc acagcttggt acacgaatcg
     1261 atgaagcatt tcttcgagtt cttcccgagc aatgctcacc ccatgggcat cctctcggcg
     1321 atggtgaact cattgagcac cttctatccc aagagccaga taccgcaccg ttcggaatcg
     1381 gagcggatgc ggaccatcta ccggctcatc gccaagatgc ccacgctggc cgcgatcagt
     1441 tacaagaaca accagggaca cccctacatg taccccgata acaaactggg gtacgtggag
     1501 aatttcttgt acatgatgta cgcgctgccg accgagtcgt acaaggcgga ccccgtggtg
     1561 gtggacgccc tgaacaagct gctgatcctg cacgccgacc acgagcagaa ctgcagtgcg
     1621 agcacggtgc gcatggtggg cagcagccag gccaacctgt acagcgcggt gagcgcgggc
     1681 atcgcggcct tgtggggtcc cctgcacggg ggtgccaacc aggaggtgat cgagatgctc
     1741 gagaccatcc gcaacgatgg cggggatatc cgcaaatggg tggacaaggc caaggacaag
     1801 aacgacccct tccgcctgtt cggattcggc caccgggtgt acaagaactt cgatccgcgc
     1861 gcccggatca tcaagagtgc ctgcgatcgc gtgctcgcga agatcggccg caaggatccg
     1921 ctcctcgaca tcgcccgcaa actcgaggaa ctcgcgctca aggatccgta cttcgtcgag
     1981 cgcaagctct acccgaacgt cgacttctac agcggcatca tcatgcgcgc catcggcatt
     2041 ccggtgaaca tgttcacggt gatgttctcc atg
//
LOCUS       DAOQOC010000524         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35467_length_2071_cov_3.333829, whole genome shotgun sequence.
ACCESSION   DAOQOC010000524 DAOQOC010000000
VERSION     DAOQOC010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35467_length_2071_cov_3.333829"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..352
                     /locus_tag="PL106_08885"
     CDS             107..352
                     /locus_tag="PL106_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNA33222.1"
                     /translation="MTFSHHVYILLCAEGLYYVGCTSDLDARMERHGQGAMHYTRDKL
                     PVRCVAAFSFPDKYKAFAFEKYLKSGSGRAFMSRHLI"
     gene            583..1230
                     /locus_tag="PL106_08890"
     CDS             583..1230
                     /locus_tag="PL106_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33223.1"
                     /translation="MIRTLALGALFAVTTSASAQMGYSGAAMRTILKKGDFSALQDQT
                     SVLLVYDYSDMTVGKFTEENYVNKKRDEYNKKESGRGDTWAGKWVGARTELYEPKFEE
                     LLNDRLASGKSALKAGKDKTDAKYVIKVHTTETEPGYNVGVSRVPGYITAVMTLYEAS
                     APDNVLGEVEIERAPAQGAMGFDFDAGGRIAEGYAKMGKEFGTWLTKKDWGRKHK"
     gene            complement(1311..>2071)
                     /locus_tag="PL106_08895"
     CDS             complement(1311..>2071)
                     /locus_tag="PL106_08895"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNA33224.1"
                     /translation="RSSFGNRFSNFWFKVETGITLPDTQTGYRGWPLKAVRSFRTTSD
                     RFGFEVESIVKLAWRGTRFHSVPVTVRYDYPERVSHFKPFLDFTRISLLNTWLVIQAL
                     LWYWPKKVFFEGGWRAGLRAEAVRPDESSLRKALSIGFGLFMGIVPIWGFQLLVGIPL
                     AYLFRLNRVLFVAAAHISIPPMIPVILYASYLTGALFTAEDAYLPTSFNELTLASIHA
                     NVVQYAIGALVLATAAGLAGLLVSWPLMRMLRRR"
BASE COUNT          435 a          646 c          625 g          365 t
ORIGIN      
        1 gagcaccgca ccagcggtgc tcgtcgcgta ggcgggcttc gcctcttttt ccgccctcgg
       61 cccgatcttc ggctcggcta aggcggacgc ggtccacctg ttcgggatga ccttttcgca
      121 ccatgtatac atcctgctgt gtgcggaggg cctctattac gtgggttgca caagcgatct
      181 ggacgcccgt atggagcgcc atggccaagg cgcgatgcat tacacacgcg ataagcttcc
      241 cgtgaggtgc gtagccgcat tcagttttcc ggacaaatac aaggcgttcg cgttcgagaa
      301 gtacctgaag agcggctcgg gcagggcgtt catgtccagg catctgatct gatcgcctcg
      361 ccgaagcgac gatcaccgca ccagcggtgc tcgtcgcgta ggcgggcttc gcctcttttt
      421 ccaaaacccg tgcacgcttc ggctcggcta cggcggacgc gcttgatgcg ttttcgggca
      481 catgtgaata cgattcaggg tcaatttcca ggatcgttcc gaagtcaggc acggatgttg
      541 ccatcaagcc tactttcgcc acgaaacccc aacaaccaac acatgatccg cacacttgcg
      601 ctcggcgcgc tcttcgccgt aacgacatcg gcctccgctc agatgggtta ctccggggcc
      661 gccatgcgca ccattctgaa aaagggtgat ttttccgccc tgcaggacca gacctccgtg
      721 ctgctcgttt acgattactc ggacatgacc gtcggcaagt tcaccgagga gaactacgtg
      781 aacaagaagc gcgacgaata caacaagaag gagagcggcc ggggcgatac ctgggccggg
      841 aaatgggtgg gagcccgcac cgaactctac gaacccaagt tcgaggaact gctcaatgac
      901 cgcctggcat ccggcaagtc cgcactgaag gcgggcaagg acaagaccga cgcgaaatac
      961 gtgatcaagg tgcataccac cgagaccgaa cccggctaca acgtgggtgt ttcacgcgta
     1021 ccgggctata tcaccgcggt gatgaccctg tacgaggcca gcgccccgga caatgtgctc
     1081 ggcgaagtgg agatcgagcg tgcgcccgcg caaggggcca tgggcttcga tttcgacgct
     1141 ggtgggcgga tcgctgaagg ctatgccaaa atgggcaagg aattcggtac ctggctcacg
     1201 aaaaaggact ggggccgcaa gcacaagtag gctcagaacg ggatcttctc aggacgcccc
     1261 ggccgccatg gatgccgggg cgttctcctt ttgtggggtg cccgttccga tcaacgtcgg
     1321 cgcagcatcc gcatcaacgg ccacgacacg agcagcccgg ccagcccggc cgccgttgcc
     1381 agcaccaacg cgccgatggc gtattgcacc acgttcgcgt ggatgctcgc caacgtgagc
     1441 tcgttgaaac tggtcggcag gtacgcatcc tccgcggtga aaagggcccc ggtgaggtag
     1501 ctcgcgtaca ggatcaccgg gatcatgggc ggaatgctga tatgtgccgc cgccacgaac
     1561 agcacgcggt tgagccggaa caaataggcc agcgggatgc ccaccagcaa ctggaagccc
     1621 cagatgggca cgatgcccat gaacaggccg aagccgatcg acagggcctt gcgcaaggaa
     1681 ctctcgtccg gtcgaacggc ctcggcgcgc aatccggccc gccagccgcc ctcaaaaaat
     1741 accttcttcg gccagtacca cagcagggcc tggatcacca gccaggtgtt cagcaggctg
     1801 atccgcgtga aatccaggaa gggtttgaag tgcgacacgc gctccgggta gtcgtaccgc
     1861 actgtcaccg gcacactgtg gaaccgtgta ccccgccaag ccagtttcac gatactctcc
     1921 acctcgaagc cgaaacggtc gctggtggtc cggaacgagc ggacggcctt caggggccag
     1981 ccccggtagc ccgtctgcgt gtccggcagg gtgatccccg tttccacctt gaaccagaaa
     2041 ttgctgaaac ggttgccgaa ggaactcctt c
//
LOCUS       DAOQOC010000525         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35491_length_2070_cov_3.628784, whole genome shotgun sequence.
ACCESSION   DAOQOC010000525 DAOQOC010000000
VERSION     DAOQOC010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35491_length_2070_cov_3.628784"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1317
                     /gene="rocD"
                     /locus_tag="PL106_08900"
     CDS             58..1317
                     /gene="rocD"
                     /locus_tag="PL106_08900"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNA33225.1"
                     /translation="MQESRTDQRSKTRIAIDLENRYGAHNYHPLPVVLERGKGVFVWD
                     VEGKRYYDFLSAYSAVNQGHCHPRLVKAMQQQAETLTLTSRAFYNSDLGNYAEYVCTL
                     FGYGRMLPMNTGAEAVETAIKMARRWGYQKKGIAKDRAKVVVCAGNFHGRTITIVSAS
                     TDPDSNSDFGPYTPGFVVVPYNDIPALEKALADPDVCAFLFEPIQGEAGVFVPDDDFI
                     ATAIRLCRARNVLSMADEVQTGIARTGRMLCTEHSGERPDVTILGKAVSGGMYPVSLV
                     LADSGVMDVFTPGSHGSTFGGNPMAARMAMEALEIVKQERLAENADRLGRIFRTGMQE
                     LVDEFPYVKLVRGKGLLNALVIEPRTAVDGSPKTAWELCLLLRDNGLLAKPTHGDIIR
                     FAPPLVITEEQLMECMRIIALSVRQFA"
     gene            complement(1321..>2070)
                     /locus_tag="PL106_08905"
     CDS             complement(1321..>2070)
                     /locus_tag="PL106_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33226.1"
                     /translation="TEEGITLIRSRLTPGTGIGGLYLPVGYNNIFFSKPRMMARFGLL
                     MLAQGTWNGDAILADENYYNAMITPSQALNPAYGYLWWLNGQSAFMLPGSQLVFPGTL
                     MPNEPADAFNALGKDAQILNVVPSQGLVVVRMGETPDDNNGAVTPVFNDAIWELLNAI
                     LCTDTGMPDADPGTARAMPNPANDHIDLAFDPALGPGDLRIIDAMGTVRGSRIGVQGR
                     LTMDVSGLSSGTYTVLFHGQAGTSVMKFVKE"
BASE COUNT          406 a          617 c          662 g          385 t
ORIGIN      
        1 ctgtgaacgc atcggtatct tcgccgggcg ccagcagcgg aaatcggcgc ctatcacatg
       61 caggaaagtc ggaccgatca acgatcgaag acccggatcg ccattgatct ggagaacagg
      121 tacggggcgc acaactatca cccgttgccc gtggtactcg aacgtggtaa aggcgtgttc
      181 gtgtgggatg ttgagggcaa gcggtactat gacttcctga gcgcgtacag tgccgtgaat
      241 cagggccact gccacccccg attggtgaag gccatgcagc aacaggcgga gaccctcacg
      301 ctcacgagcc gtgcattcta caacagcgac ctgggcaatt acgccgaata cgtgtgcacc
      361 ctgttcgggt acgggaggat gctgcccatg aacaccggtg cggaggcggt ggaaacggcg
      421 atcaagatgg cgcgtcgttg gggttaccaa aagaaaggga tcgccaagga ccgggcgaag
      481 gtcgtcgtgt gcgcgggcaa tttccatggc cgaaccatca ccatcgtcag cgccagcacc
      541 gacccggaca gcaacagcga tttcgggccg tacacgccgg gtttcgtggt ggtcccgtac
      601 aacgatatcc ccgcactgga aaaggccctg gccgatccgg atgtctgcgc cttcctgttc
      661 gaacccatcc agggcgaagc aggcgtgttc gtgccggacg acgactttat cgcgacggcc
      721 atccgcctgt gcagggcccg gaacgtgctc tccatggccg atgaggtgca gaccggtatc
      781 gcccgtaccg gtcggatgct ctgcaccgaa cacagcggcg aacgtcccga cgtcaccatt
      841 ttgggcaagg cggtgagtgg cggcatgtat cccgtaagct tggtattggc ggacagcggt
      901 gtgatggatg tcttcacgcc cggaagccac ggcagcacat tcgggggcaa cccgatggcg
      961 gcgcggatgg ccatggaggc actcgagatc gtgaaacagg aacggctggc cgagaacgcc
     1021 gaccggctcg ggcggatctt ccgcaccggg atgcaggaat tggtggatga gttcccctac
     1081 gtgaaactgg tgagaggcaa ggggctgctg aacgcgctgg tgatcgagcc gcgcacggcg
     1141 gtcgatggat cgccgaagac cgcatgggaa ctctgtttgt tgctgcgcga caatggcctt
     1201 ctcgcaaagc cgacccacgg ggacatcatc cgtttcgctc cccccttggt gatcaccgaa
     1261 gagcaactga tggagtgcat gcgcatcatc gcgctcagcg ttcgccagtt cgcataggat
     1321 tcactccttc acgaatttca tcacgcttgt accggcctgg ccgtggaaca gcacggtgta
     1381 tgtcccgctg ctcaggccgc tcacatccat cgtgagccga ccttgaaccc cgatccgcga
     1441 gccgcgcacg gtacccattg catcgatgat gcgcaggtcg ccagggccca gtgccggatc
     1501 gaacgccagg tcgatgtggt cgttggcggg gttgggcatg gcccgggctg ttccgggatc
     1561 ggcatccggc ataccggtat cggtgcagag gatggcgttc agcaattccc agatggcatc
     1621 attgaagacc ggggtcaccg ccccgttgtt gtcgtcgggg gtttcgccca tgcgcaccac
     1681 taccaggccc tgcgacggta ccacgttcag gatctgcgca tccttgccca gtgcgttgaa
     1741 ggcatctgcc ggctcgttcg gcatcaaggt gcccgggaaa acgagctgtg atccgggcag
     1801 catgaaagcg ctttgtccgt tcaaccacca caaatagccg tatgcgggat tgagcgcctg
     1861 cgatggcgtg atcatcgcgt tgtaatagtt ctcgtcggcg aggatcgcat ccccgttcca
     1921 ggtgccctgg gccagcatca gcaggccgaa ccgggccatc atgcgcggct tgctgaagaa
     1981 gatattgttg tagcccacag gcaggtacag cccgccgatg cccgtgcccg gggtgaggcg
     2041 gctcctgatc aacgtgatgc cttcctcggt
//
LOCUS       DAOQOC010000526         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35595_length_2067_cov_3.294235, whole genome shotgun sequence.
ACCESSION   DAOQOC010000526 DAOQOC010000000
VERSION     DAOQOC010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35595_length_2067_cov_3.294235"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="PL106_08910"
     CDS             <1..1579
                     /locus_tag="PL106_08910"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33227.1"
                     /translation="ILALARWISDYYLAPIEQGRGRTLQLTHRWAEAIEAFSNAEAHA
                     RKLGMQDLWPMCMAGRAGIYSEMDSSQRAKFGLLNAYEQARAILDSALADIAPTGQVQ
                     YEAAVERIYGDVATRFKDPATAVPHLQRAQDLFSRVGHGIGVADAQLGLGRGLLEQGD
                     PAPAADILQRAASFFKSNDMRDMQAQALFSLHQAQQRTGRTNEALASLQEAYRLSDLA
                     RNDSTRNAMADQEAGFKYKAVQDSLSLAHRVAMSEQQVITAEAETDAARARTTTVAVS
                     AVGILLLASGLFFHRSREQRIKNATAIAIAQANEQLAEEKLRTAEYQNRALRAQMEPH
                     FIRNTIALAIARLNNGTPTATDDASELLARFSDWIDKVLVTSQHATHALRDELNTLGH
                     YLELQRLRSKGKLQYTVTVDPALDPDLVDVPPMLLQPLIENAIEHGLEPRGGEGRITL
                     SAVQREGSLVLCAEDDGVGRNHPKPEGHVRKKGASVSTANIREQLAMLAARTGKRAEL
                     RIIDLPQGTRAEVWLPL"
     gene            1710..>2067
                     /locus_tag="PL106_08915"
     CDS             1710..>2067
                     /locus_tag="PL106_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA33228.1"
                     /translation="MSRKYNAIIVEDNEEMATYLKNLIGTRHKNIVVSAVANNVPDAA
                     EVIARQPPDLLFLDVELGDMKGFDLLERLRNITFPVIFTTGKSEYAIPALNAHLAYSS
                     FLVKPVLGPGLDTEVIR"
BASE COUNT          429 a          710 c          603 g          325 t
ORIGIN      
        1 catcctcgcg ctggcgcggt ggatttccga ttactacctg gccccgatcg agcaaggcag
       61 agggcggacc cttcagctta cgcaccggtg ggcggaagcg atcgaagcct tctccaacgc
      121 cgaggcacac gcccgaaaac tcggtatgca ggacctctgg cccatgtgca tggcaggccg
      181 tgccgggatc tactccgaga tggacagctc ccaacgcgcc aagttcggcc tgctgaacgc
      241 ctatgaacag gctcgcgcca tattggacag cgcactggcg gacattgccc cgaccggaca
      301 ggtccaatac gaagcagccg tggagcgtat ctacggtgat gtggccacac gcttcaagga
      361 tcccgccacg gcggtaccgc atctgcagcg cgcccaagac cttttctcgc gtgttggcca
      421 tggcatcggt gttgccgacg cgcagctcgg cctgggacgt ggattgctgg agcaaggcga
      481 tccggcccct gccgcggaca tccttcaacg ggcggcaagc ttcttcaagt ccaacgacat
      541 gcgcgacatg caggcccagg ccctcttcag tcttcaccag gcccagcagc gcaccggcag
      601 aacgaacgag gcactggcct ccttacaaga ggcctaccgg ctgagcgacc ttgcccggaa
      661 cgacagcacg cgcaacgcca tggccgacca ggaggcgggg ttcaaataca aagccgtgca
      721 ggattcgctc agcctggcac atcgcgtggc catgagcgaa cagcaggtga tcaccgccga
      781 ggcggagacc gatgccgcgc gagcacgcac caccaccgtg gccgtctcag ctgtcggcat
      841 ccttttgctt gccagcggtc tcttcttcca tcgcagccgc gagcaacgca tcaagaacgc
      901 caccgccatc gccatcgccc aagcaaacga acaactggcc gaagaaaagc tcaggaccgc
      961 ggaataccag aaccgcgccc tccgcgcgca gatggagccg cacttcatcc gcaacaccat
     1021 cgctctggcc atcgcccggc tcaacaacgg cacacccacg gccacggacg atgccagcga
     1081 gttgctcgcc cgtttctccg actggatcga caaggtgctg gtgaccagtc agcacgccac
     1141 gcatgcgctg cgcgatgagt tgaacaccct tggccactac ctggaactcc agcggctgcg
     1201 ctcgaaaggc aaattgcagt acaccgtaac cgtggaccct gccctggacc cggacctggt
     1261 ggatgtgccg cccatgctct tgcagccctt gatcgagaac gccatcgagc acgggctgga
     1321 accccggggc ggtgaaggtc ggattacctt gagcgccgtg cagcgcgagg gttccctggt
     1381 gctctgcgcc gaggacgacg gagtaggccg taaccatccg aagcccgaag gccatgtgcg
     1441 caagaagggc gcctcggtga gcacggccaa catccgcgag caactggcca tgctggccgc
     1501 gcgcacaggc aaacgtgccg aactgcggat catcgatctg ccccagggca cacgcgccga
     1561 ggtgtggctg ccgctatgat ccccgaccgc ccaccgcttt cacgcccgaa cccaccggat
     1621 acctcatctt gggttgttcc gtacgggggt gcatgcagct tgcacgccac aaacgccacc
     1681 gcatggaaca gacccctatc ttgcctgcta tgagccggaa atacaacgcc atcatcgtgg
     1741 aggacaacga ggagatggcc acctacctga agaacctgat cggcacccgc cacaagaaca
     1801 tcgtggtgag tgccgtggcg aacaacgtgc cggatgccgc cgaagtgatc gcccgccagc
     1861 cgcccgacct gctcttctta gatgtggaac tgggcgacat gaaagggttc gacctgctgg
     1921 aacgcttgcg caacatcacc ttccccgtga tcttcaccac cggtaagagc gaatacgcca
     1981 ttcccgccct gaacgcgcac ctggcctact cctccttcct ggtgaaaccc gtgctggggc
     2041 ctggcctgga cacggaggtg atccgcg
//
LOCUS       DAOQOC010000527         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35681_length_2064_cov_3.347934, whole genome shotgun sequence.
ACCESSION   DAOQOC010000527 DAOQOC010000000
VERSION     DAOQOC010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35681_length_2064_cov_3.347934"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..444)
                     /locus_tag="PL106_08920"
     CDS             complement(247..444)
                     /locus_tag="PL106_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33229.1"
                     /translation="MTRLQVSASISDLIVPRCDDARPASTDRLIGNWGGRIYVEGVRS
                     FDLVGNGDQDVLVYLDTDYSY"
     gene            721..1176
                     /locus_tag="PL106_08925"
     CDS             721..1176
                     /locus_tag="PL106_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33230.1"
                     /translation="MNAFRNLLISLVFLLVVGEGRASEPRQYRLTMNALVASTGGSLD
                     GAHALLFVGGQLMDSTSLEDGGFTLDLPTGMDAVLEVRMPGHITKQVVLATAGLTHDQ
                     RFDCDILLFPGNGMVERAGRITCDPATGGVIVVHDPRTTGSAAQAVARP"
     gene            complement(1278..1733)
                     /locus_tag="PL106_08930"
     CDS             complement(1278..1733)
                     /locus_tag="PL106_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNA33231.1"
                     /translation="MTLQERIDADIRTAMLAREKDRLNTLRAIKSAILLELTKEGSTG
                     SVGPEAGLRILQKLHKQRTEAAAIYHQQGRADLANEDETQARIIESYLPARMGIAEVE
                     AAVQRILAGIGANGMADMGRAMKSVNAELAGKADGGTIAAAVKKILSGG"
     gene            complement(1770..1967)
                     /locus_tag="PL106_08935"
     CDS             complement(1770..1967)
                     /locus_tag="PL106_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33232.1"
                     /translation="MRHPNGLSEYEKEPAFKRKNPAFTEVQHSSESNVSRYSLSEETD
                     ENGDRSVQLRKNNPAIHDLPD"
BASE COUNT          333 a          640 c          659 g          432 t
ORIGIN      
        1 gcccgaggcg ttgccgcaaa gtgcagtgac cagtgtggtt acgcaggcgg cgcggaggag
       61 gatgctggtt ttcatggccg ggactcgttg agggtgcttt gtcgtgatcg cgaggtcaaa
      121 gcaaccgcgt caggcaggga agcgtatgcg gccatgcgca ggtggcggtg accgatgagc
      181 aggtggcgga aaagggcctg gccctcgttt tcagctgagg gacctgtccg gcaccaagtg
      241 tcatgctcag tatgaatagt ccgtgtccag gtagacgagt acgtcctggt ccccattacc
      301 gaccaagtca aaggagcgca cgccctccac gtatatgcgg cctccccagt tgccgatgag
      361 tcgatcggta ctggctgggc gtgcatcatc gcagcggggg acaatgaggt cagaaatact
      421 tgctgatacc tgaaggcggg tcaaggagtg gccgcgttgt tgtccgacat cttggcgaac
      481 ggaaagtgga agaatgagcg gcccggctgc gctggaacat cgttcgtggc gctcggtccg
      541 ggacatgccg ggaatacggt cctgtcggta tcggtcggtc gatggaactg gcccggcatc
      601 caaaggtgcc gccggcatgc catctgcgca cccgccatcg ccacttgctc aacggcgctc
      661 acgcacgcag ttgtgccgcg cttgtttcgc cccgtcaaca acaacgaacc ccccgccccc
      721 atgaatgctt tccgcaacct gctcatctcc ctggtctttc tcctcgtcgt tggtgaggga
      781 agagcctcgg aaccccgtca gtaccgactc accatgaacg cattggtggc cagcaccgga
      841 ggaagcttgg acggtgctca cgcgttgctt ttcgtcggag gtcaactgat ggacagcacc
      901 tccttggagg atggtggttt cactttggac ctgcccacgg gtatggatgc ggtgctcgag
      961 gtgcgcatgc ccggacacat caccaagcag gtggtactgg ccaccgcggg gctcacccac
     1021 gaccagcgct tcgactgcga catcctgctc ttccccggca acggcatggt ggagcgggcc
     1081 ggccgcatca cctgcgatcc cgccacgggc ggagtgatcg tggtacatga tcccagaacc
     1141 accggcagtg ccgcacaagc ggtggccagg ccgtaacgct gcgccgaccc ctgcgcccaa
     1201 agggagaagg gccgtgccgc cgtgaggtgg aacggtcctt cgtgttgtcc tcggcgccgg
     1261 agggcgaccg gcgcggttca gcctcccgaa agtatttttt tcacagcggc cgctatggta
     1321 ccgccatcgg ccttgcccgc gagttcggcg ttcacgcttt tcatcgcacg acccatgtcg
     1381 gccatgccgt tggccccgat cccggcgagg atgcgttgca ccgctgcttc cacctccgcg
     1441 atgcccatac gcgcgggcag gtagctttca atgatgcgcg cctgcgtctc gtcctcgttg
     1501 gccaggtccg cacggccctg ctggtggtag atggcggccg cctccgtgcg ttgcttgtgc
     1561 aatttctgca ggatgcgcag gccggcctcc ggtcccacgc tgccggttga accctccttg
     1621 gtgagttcca gcaggatcgc actcttgatc gcgcgcaggg tgttcagccg gtccttctca
     1681 cgcgccagca tggccgtcct gatgtccgcg tcgatgcgct cttgtagcgt cacggtccct
     1741 ggtgtttccg gtccgaacga agcctggccc taatcaggca ggtcgtggat ggcggggttg
     1801 ttcttgcgca gttgcacgct gcggtcaccg ttctcatcgg tctcctcgct cagcgagtac
     1861 cggctcacat tgctctcgct gctgtgctgg acctcggtga acgccgggtt cttgcgcttg
     1921 aacgccggtt ctttttcgta ctcggacagg ccgttgggat ggcgcatgcg ctgcgtcatt
     1981 tcgcgtatgc gtgccagacg ctcctcggac cggcgctgaa cgtccgatga tttatacagg
     2041 tggtggcatt attttggcta atgc
//
LOCUS       DAOQOC010000528         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35697_length_2063_cov_5.461155, whole genome shotgun sequence.
ACCESSION   DAOQOC010000528 DAOQOC010000000
VERSION     DAOQOC010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35697_length_2063_cov_5.461155"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PL106_08940"
     CDS             <1..838
                     /locus_tag="PL106_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNA33233.1"
                     /translation="FGLSDLHQLRGRVGRSNRKAYCYLLAPSPHLLPDLSRKRLQAIE
                     QFSDLGSGIHIAMKDLDIRGAGDLLGAEQSGFVNDIGFETYHKILEEAVRELKDEHFK
                     ELFADAQEGSHSLARGSRRDQSDDCIIETDLTMLIPNSYVSETAERLALYRRLDDIKD
                     ENELQAFANEVQDRFGPIPHEVEELMEGIRLRWLGQRMGLEKMVLKNGDLRGTFIADQ
                     QHPFFSGDGFQAVLRAVQAQPKRYKVYEKSGKLKITVAAVKNLGEAKRALEIVVGVKE
                     SV"
     gene            complement(1015..1371)
                     /locus_tag="PL106_08945"
     CDS             complement(1015..1371)
                     /locus_tag="PL106_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNA33234.1"
                     /translation="MSIKPEFADKIFDGSKRYEFRRMIFKQPAVKKVVVYASAPVQMV
                     IGEFEIESILEHPPEELWRRTREGAGISKEFFMSYFGDKVVGYAIRIKATKRYRKPLC
                     LKSDYNLKPPQSYLYL"
     gene            complement(1380..>2063)
                     /locus_tag="PL106_08950"
     CDS             complement(1380..>2063)
                     /locus_tag="PL106_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33235.1"
                     /translation="ERTDEQKRLIDLLTTWGFTKHGVKRSQSGEELVYVRNMRKDNPA
                     TEPKHRYPWLRKDRKVYVVPIRPEFHTELLPDSILRTEDLSAQQDGEPHQNAISKVYV
                     SHTMNRDLQPGDTLIFYRTGGMYKGVATTIGIVENVINPVPDFDTLRFHSRKRSVLNV
                     DQLREFWERNPRNRPCVVNFLHAYPLKKRPNLKALLEAGIFPNMDIVRTINEVPFDSL
                     KMLLILAGL"
BASE COUNT          432 a          609 c          608 g          414 t
ORIGIN      
        1 cttcggcctc agcgacctgc accagctgcg cggccgcgtg gggcgcagca accgcaaggc
       61 ctactgctac ctgctcgcgc ccagcccgca cctgctgccg gacctcagcc gcaaacgcct
      121 gcaagccatc gagcagttca gcgacctcgg cagcggcatc cacatcgcca tgaaggacct
      181 cgacatccgc ggcgcgggcg acctgctcgg cgcggaacag agcggcttcg tgaacgacat
      241 cggattcgag acctaccaca agatcctcga agaggccgtg cgcgaactga aggacgagca
      301 cttcaaggaa ctcttcgccg atgcacagga aggcagtcat tctttggccc gcggatcacg
      361 ccgcgaccag agcgacgact gcatcatcga aacggacctc acgatgttga tccccaacag
      421 ctacgtaagc gagaccgccg aacgcctcgc cctctatcgc cgcttggacg acatcaagga
      481 cgagaacgaa ctgcaagcct tcgccaacga agtgcaggac cgcttcggtc ccatccctca
      541 cgaggtggag gaactgatgg agggcatccg attacgctgg ctgggccaac gcatgggctt
      601 ggagaagatg gtgctgaaaa atggcgacct gcgcggcacc ttcatcgcgg accagcagca
      661 ccccttcttc tccggcgatg gcttccaggc cgtgctgcgc gcggtacagg cacagcctaa
      721 acgttacaag gtgtacgaga agagcggcaa actgaagatc accgtggcgg cggtgaagaa
      781 cctcggggag gcgaagcggg cgttggaaat agttgttggg gtgaaggaga gcgtgtaggt
      841 ggtttgggcg ttccggcgct caaattcatg cggacccggg gacaacgcta tagccggatt
      901 tgtcagggcc agggtccacg gcgatgttga ggcttccttc ctcggccacc tcgctgcttg
      961 agaccccatt gcccttcagg tatcggcttg ctactttgcc ggcccataaa atgtctacaa
     1021 atacaagtag ctctgcggcg gcttaaggtt atagtcggac tttaagcaca gaggtttgcg
     1081 gtagcgcttg gtagccttga tacggattgc gtaacccacg accttatcgc cgaagtagga
     1141 catgaaaaat tccttgctga tgcctgcgcc ttcgcgagta cggcgccaca gctcttcggg
     1201 cggatgctcc agtatgctct cgatctcgaa ctccccgatc accatttgta ccggggcgct
     1261 ggcatatacc accaccttct tcacggcggg ctgcttgaag atcatgcggc ggaactcgta
     1321 acgcttggag ccgtcgaaga tcttgtctgc gaactccggc ttgatggaca tgagaacttt
     1381 catagcccgg ccaagatgag gagcattttg agcgaatcga aaggaacctc gttgatggtt
     1441 ctcactatgt ccatgttagg aaagatgccg gcctccagca aagccttgag attcggacgt
     1501 ttcttgagcg gataagcatg gagaaagttg accacgcacg gacggttccg gggattacgc
     1561 tcccagaact cgcgcagctg atcaacgttg agcactgatc gcttccggct gtggaagcgg
     1621 agcgtatcga agtccggaac aggattgatc acgttctcca cgatgccgat ggtcgttgcc
     1681 actcccttgt acattccacc ggtacgatag aagatcaagg tatcccctgg ctgaaggtcc
     1741 cgattcatgg tgtgcgacac gtacacctta ctgatcgcgt tctggtgcgg ctcaccatcc
     1801 tgttgggcgg acaagtcctc ggtgcgcagg atcgaatcgg gcagcagctc cgtgtggaat
     1861 tccggccgga tcggcaccac atagaccttc ctgtcctttc gcaaccaggg gtaacggtgc
     1921 ttgggctcgg ttgccgggtt gtccttgcgc atgttgcgca cgtacaccag ctcttctccg
     1981 gactgcgacc gcttgacacc gtgcttggtg aagccccaag tcgtgagcag gtcgatgagg
     2041 cgcttctgct cgtcggtccg ctc
//
LOCUS       DAOQOC010000529         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35702_length_2063_cov_4.288347, whole genome shotgun sequence.
ACCESSION   DAOQOC010000529 DAOQOC010000000
VERSION     DAOQOC010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35702_length_2063_cov_4.288347"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PL106_08955"
     CDS             <1..201
                     /locus_tag="PL106_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33236.1"
                     /translation="RSGMFLVTAGVSALVAAGSAWLIVRHAERDRPKFFDSEKVKFDG
                     LVWLPSSEQQGVWMAGLHLDIR"
     gene            204..710
                     /locus_tag="PL106_08960"
     CDS             204..710
                     /locus_tag="PL106_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33237.1"
                     /translation="MMKHAAGSGQQAAGTGHRAVDDGSRRIRLARYCLLPAACCLLLA
                     SCYKDDLDPAALTNNPFDPGYNGPAIFTFDTTYVEVLTTFPLIQRQVFQFHVNSGLFL
                     AEQAYSVRVHDQVSGTTSMVEQLPAGSDVLRYYRLEFTHGQEYCMGLALSNNTHDGRT
                     ETVCGFLP"
     gene            707..>2063
                     /locus_tag="PL106_08965"
     CDS             707..>2063
                     /locus_tag="PL106_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNA33238.1"
                     /translation="MSNEHNEMVPDEEAMNGTNEDGARPGVPGAGHSGQYSVSGMYRE
                     WFLDYASYVILERAVPALYDGLKPVQRRILHAMDDLDDGRYNKVANIIGHAMQYHPHG
                     DASIGDALVQLGQKDLLIDCQGNWGNTLTGDAAAAPRYIEARLSKFAHDVVFNPKTTD
                     WQLSYDGRNKEPVHLPVKFPLLLAQGAEGIAVGLSCKILPHNFNELIDASIAVLRKRS
                     FDLVPDFPTGGLADASNYNNGERGGRIRCRARIRKEDNKTLVISEIPFGTTTTSLIES
                     ILKANEKGKIKVRHIEDNTAENAEIVIHLAAGVSPDTTIDALFAFTDCEVSLAPNAVV
                     IENDKPRFVDVKELLRISTENTLRLLEMELRIRKGELEEQWHFASLERIFIEKRIYRR
                     IEEAETWDEVIGTIHKGLKPHIQDLRRSVTDEDVVKLTEIRIKRISKFDSFKADEHIR
                     HI"
BASE COUNT          443 a          640 c          619 g          361 t
ORIGIN      
        1 aggagcggga tgttcctcgt taccgcgggt gtgagcgcct tggtggccgc gggcagtgcc
       61 tggctgatcg tgcgccatgc cgaacgcgac cgcccgaagt tcttcgacag cgagaaggtg
      121 aagttcgacg ggctcgtctg gttgccttca agcgagcaac agggcgtgtg gatggcaggc
      181 ttgcatcttg atatccgatg acgatgatga aacacgcagc gggcagcggg cagcaggcag
      241 caggcactgg acatcgtgcg gttgacgatg gttcccggcg catacggctg gccaggtact
      301 gcctgctgcc tgctgcctgc tgcctgctgc tggcctcctg ctacaaggac gacctcgacc
      361 ccgcggcact caccaacaat cccttcgatc cggggtacaa cggaccggcg atcttcacgt
      421 tcgataccac gtacgtcgaa gtcctcacca ctttcccgtt gatccagcgc caggtcttcc
      481 agttccacgt gaacagcggt ctgttcctgg ccgagcaagc gtacagtgtg cgcgtgcatg
      541 accaggtcag cggtaccacc agcatggtgg aacagctgcc ggcgggcagt gatgtgctgc
      601 gttattaccg cttggagttc acccacggcc aggagtactg catgggactg gccctttcca
      661 acaacaccca cgatggccgc accgaaaccg tgtgcggctt cctgccatga gcaacgaaca
      721 caacgagatg gttcccgatg aggaggccat gaacggtacg aacgaggatg gtgcacgtcc
      781 cggtgtgccc ggggccggtc acagcggcca gtacagcgtg agcggcatgt accgcgagtg
      841 gttcctggac tacgccagct atgtgatcct ggaacgtgcc gtgcccgccc tgtacgatgg
      901 gctcaaacct gtgcaacgcc gcatcctgca cgccatggac gatctggacg atggccgcta
      961 caacaaggtg gccaacatca tcggccatgc catgcagtac cacccgcacg gtgatgccag
     1021 catcggcgat gcattggtgc agttgggcca gaaggacctg ctgatcgatt gccagggcaa
     1081 ctggggcaat accctcaccg gcgatgccgc tgccgcaccc cgctacatcg aggctcgcct
     1141 cagcaagttc gcccacgacg tggtcttcaa ccccaagacc accgattggc aactcagcta
     1201 cgacggccgg aacaaggaac cggttcacct cccggtgaag ttcccgttgc tgttggcaca
     1261 aggggcggag ggcatcgcgg tgggtctcag ctgcaagatc ctgccgcaca acttcaacga
     1321 actgatcgat gccagcatcg ccgtgctacg caagcgatcc ttcgatctgg tgcccgactt
     1381 ccccacgggt ggactggccg acgccagcaa ctacaacaac ggcgagcggg gtggtcgcat
     1441 ccgctgccgc gcgcgcatcc gcaaggagga caacaagaca ctggtgatat ccgagatccc
     1501 cttcgggacc accaccacca gcctgatcga gagcatcctg aaagccaacg aaaagggcaa
     1561 gatcaaggtg cgccacatcg aggacaatac cgccgagaac gcggagatcg tcattcacct
     1621 ggccgcaggc gtttcgcccg ataccaccat cgacgcgcta ttcgcattta ccgattgcga
     1681 ggtaagtctg gcgcccaatg ccgtggtcat cgagaacgac aagccgcgtt tcgtggatgt
     1741 gaaggagttg ctgcggatca gcaccgagaa taccctgcgg ctgctggaaa tggagttgcg
     1801 gatccgcaag ggcgaattgg aggaacagtg gcacttcgcc tcattggaac ggatcttcat
     1861 cgagaagcgc atttaccgac gcattgagga ggctgagacc tgggacgagg tgatcggcac
     1921 catccacaaa ggcctcaagc cgcacatcca ggacctgcgc cgatccgtta ccgatgagga
     1981 tgtcgtgaag ctgaccgaga tccgcatcaa acgcatctcc aagttcgaca gcttcaaggc
     2041 cgacgagcac atccgccaca tcg
//
LOCUS       DAOQOC010000530         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35812_length_2060_cov_3.380050, whole genome shotgun sequence.
ACCESSION   DAOQOC010000530 DAOQOC010000000
VERSION     DAOQOC010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35812_length_2060_cov_3.380050"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..880
                     /locus_tag="PL106_08970"
     CDS             134..880
                     /locus_tag="PL106_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA33239.1"
                     /translation="MLNAILIDDEANAREKLAFMLEQHCATSVKLVAQARNVEEGLEV
                     IRAQRPDLIFLDVEMPGESGFDLLRRLGKADLDVIFTTAHDHYAIKAIKFAAVDYLLK
                     PIDMDQLREAVARVLEKRRSSITDSRIDQLVESTRQGAGVTSLSVPSQDGFVRVKLAD
                     IVWCEAERYFTIFHLVGGKELVATRSLGDFEELLAGSGFVRIHHGHLVGTEHIVRYIK
                     GAGGQVVMSDGKTLDVSRRKKDELMERMGR"
     gene            complement(950..1972)
                     /locus_tag="PL106_08975"
     CDS             complement(950..1972)
                     /locus_tag="PL106_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNA33240.1"
                     /translation="MLPEFRDARLWGRALTARRTRNAWRVWSSYQRAKRTKDPRIAGL
                     PISVSIEPTTACNLRCPECPSGLRAFTRPTGNLKQELFKHIIDELHRDLWALTFYFQG
                     EPFINPGFLDMVRYASERGVYTSTSTNAHFLDEANAEAAVRSGLSRLIISLDGTDQPT
                     YEAYRKEGTLSKVIEGAERVVKWKTKLKSLSPHVVFQFLVVKPNEHQIPEARALARRI
                     GVDDLWLKTAQVYDPKDDHPLIPVQDKYSRYRRTADGAWVVKNKLDDNCWKMWHSCVV
                     TWDGRVVPCCFDKDAHHVLGDLRKNSFREVWNSDAYRDFRAGLLHDRGGLEMCRNCSE
                     GSPVWA"
BASE COUNT          382 a          616 c          667 g          395 t
ORIGIN      
        1 gattggaact gctgcggcgc aacgccagca tcaacagccg catcgagacc atcgacctgc
       61 gcaacgaaca aggtgagccc accggaacgc gggtggtgat caccctctcc ctggaaaccg
      121 attgacccac cacatgctca acgccattct catagacgac gaagcgaacg cgcgggagaa
      181 gctcgccttc atgctggagc aacattgcgc aacgagcgtg aagctcgtgg cccaagctcg
      241 caacgtggag gaagggctcg aggtcattcg tgcgcaacgc ccggatctga tcttcctgga
      301 tgtggagatg cccggtgaga gcggtttcga tctgctgcgc cgtcttggca aggccgacct
      361 ggatgtgatc ttcaccacgg cacacgatca ctacgccatc aaggccatca agttcgcggc
      421 cgtggattac ctgttgaagc ccatcgacat ggaccaattg cgcgaggccg tcgcgcgtgt
      481 cctggagaag cgccgtagtt ccatcaccga cagccggatc gatcagctcg tggaatccac
      541 acggcaaggc gctggcgtga ccagtctcag tgttccatcg caggacggat tcgtgcgtgt
      601 gaagctcgcg gacatcgtct ggtgcgaggc cgaacgctac ttcaccatct tccatctggt
      661 gggtggcaaa gagctggtgg caacgcggtc gctcggcgat ttcgaagagc tcttggccgg
      721 aagcggcttc gtgcgtatcc accacggcca tctcgtgggc acggagcaca tagtccgcta
      781 catcaagggc gcgggcggac aggtggtgat gagtgatgga aagacactgg atgtaagccg
      841 aaggaagaag gacgaattga tggagcgaat gggtaggtag tgtacgaggt atttgtcgac
      901 gttaggcggg ttgtcatcac gggtctgttc agatggcgca gccgaagcct tacgcccaca
      961 ccggactccc ctcactgcag ttcctgcaca tttcgagccc gccccggtcg tgcagcaggc
     1021 ccgcacggaa gtcgcggtag gcatcgctgt tccatacctc acggaaagag ttcttccgca
     1081 gatccccgag cacgtggtgc gcgtccttgt cgaagcagca gggcaccacg cgaccgtccc
     1141 aggtgaccac gcagctgtgc cacatcttcc agcagttgtc atccagcttg ttcttcacca
     1201 cccacgcacc gtcggcggta cggcggtagc gggaatactt gtcctgtacc ggtatcaacg
     1261 ggtgatcatc cttcgggtcg tacacctgtg cggtcttcag ccacaggtcg tccacgccga
     1321 tgcgtctggc tagtgcgcgc gcctcgggga tctggtgctc gttcggtttc accacaagga
     1381 actggaacac cacgtggggt gagaggctct tcagcttcgt cttccacttc acgacgcgtt
     1441 ccgccccctc gatcaccttg ctcagggtac cttccttacg gtacgcttca taggtcggct
     1501 ggtccgtgcc atccagcgag atgatcaggc gggagagtcc actgcgcacg gcagcctccg
     1561 cgttggcctc gtccaggaag tgggcgttgg tgctcgtgct cgtgtacacc ccgcgctcgc
     1621 tcgcgtaccg caccatgtcc aggaagccgg ggttgatgaa gggctcgccc tggaagtaga
     1681 aggtgagtgc ccagaggtcg cggtgcagtt cgtcgatgat gtgcttgaac agctcctgct
     1741 tcaggttgcc ggtgggccgg gtgaaggcgc gcaggccgct ggggcattct gggcagcgga
     1801 ggttgcaggc cgtggtgggc tcgatgctca cgctgatcgg caggcccgcg atgcggggat
     1861 ccttcgtgcg cttggcgcgc tggtagctgc tccatacgcg ccaagcgttg cgggtgcgcc
     1921 gcgctgtgag ggcacggccc cagaggcggg cgtcgcgaaa ttcggggagc atggaaggac
     1981 ggcgcaaggt agcctggacc gtacgaaggg aagggccagg gtcgctcctt gtccatccgt
     2041 gccctggggc gttcccaagc
//
LOCUS       DAOQOC010000531         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35849_length_2059_cov_4.228543, whole genome shotgun sequence.
ACCESSION   DAOQOC010000531 DAOQOC010000000
VERSION     DAOQOC010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35849_length_2059_cov_4.228543"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /gene="dxs"
                     /locus_tag="PL106_08980"
     CDS             <1..1619
                     /gene="dxs"
                     /locus_tag="PL106_08980"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNA33241.1"
                     /translation="RSESAYDTFGVGHSSTSVGGALGMAVASKLKHENDRQVVAVIGD
                     GAMTAGMAFEALNHAGTANSDLLVVLNDNCMSIDPNVGALKEYLTDITTSHTYNKVKD
                     EVWKVLGKLSKFGPNAQAIAQKFDAALKTALLKQSNLFESLKFRYFGPVDGHDVERLV
                     KVLKDLRDIPGPKLLHTVTVKGKGYAPAEVGSPTVWHAPGLFNKETGEIIKVVPKSPQ
                     PPKYQDVFGHTIVELAEKNDRIVGITPAMPTGCSLNIMMKAMPDRAFDVGIAEQHAVT
                     FSAGLATQGMVPFCNIYSSFMQRAYDQVVHDVALQNLHVVFCLDRGGLAGADGPTHHG
                     AFDIAYFRCIPNMIVSAPMNEEELRNLMYTAQLKDQGPFSIRYPRGEGVMTEWKTPFK
                     EIPIGTGRRVRTGNDLAVLSIGHIGNIAAKGIDALQAEGYSIAHYDMRFAKPIDEMLL
                     HEVFGKFKYVITIEDGCIQGGMGSAVLEFMADHGYSAHVKRLGIPDKWIEHGSQKELY
                     AECGFDEVALVAAVKEMMPQKGVKKSIASA"
     gene            1632..1835
                     /locus_tag="PL106_08985"
     CDS             1632..1835
                     /locus_tag="PL106_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33242.1"
                     /translation="MRTDPRIRFETKEESNARREREFMALTPWERLALYLREITRNPP
                     TREERGEAKGNFIIWKRADGVRG"
     gene            1819..>2059
                     /locus_tag="PL106_08990"
     CDS             1819..>2059
                     /locus_tag="PL106_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33243.1"
                     /translation="MEFEAEVKVFLSLAQREGLRIIMVGGAAVNHHGYKRHSADIDLW
                     IESSPENFNRLLTVLKAIGYEVDALPTKVLAAEQNI"
BASE COUNT          449 a          608 c          653 g          349 t
ORIGIN      
        1 agcgcagtga gagcgcgtac gacacctttg gcgtaggcca cagctccacc tccgtgggcg
       61 gcgcgctggg catggccgtc gccagcaagc tcaagcacga gaacgatcgc caggtggtgg
      121 ccgtgatcgg cgatggcgcc atgaccgccg gcatggcttt cgaggctctg aaccacgcag
      181 gcacggccaa cagcgatctg ctggtggtgc tcaacgacaa ctgcatgagc atcgacccga
      241 acgtgggggc gctcaaggag tacctcaccg acatcaccac cagccatacc tacaacaagg
      301 tgaaggacga ggtgtggaag gtgctcggca agctcagcaa gttcggcccg aacgcgcagg
      361 ccatcgcgca gaagttcgat gccgcgctca agaccgccct gctcaagcag agcaacctgt
      421 tcgagagcct caagttccgc tacttcggtc cggtggacgg gcacgacgtg gagcgccttg
      481 tgaaagtgtt gaaggacctg cgcgacatcc ccggccccaa gctgttgcac acggtgacgg
      541 tgaaaggcaa gggctacgcg cctgcggagg tcggcagtcc gaccgtctgg catgcaccgg
      601 gcctcttcaa caaggagacc ggcgagatca tcaaggtggt gcccaaaagc ccgcagccac
      661 ccaaatacca ggatgttttc ggccacacca tcgtggaact ggccgagaag aacgatcgga
      721 tcgtcggcat cacaccggcc atgcccaccg gttgctcgct gaacatcatg atgaaggcca
      781 tgcccgatcg tgccttcgat gtgggcatcg ccgaacagca cgccgtcacc ttcagcgcag
      841 ggctggccac gcagggcatg gtaccgttct gcaacatcta cagctcgttc atgcagcgtg
      901 cctacgatca ggtggtgcat gatgtggcct tgcagaacct gcacgtcgtc ttttgcctgg
      961 atcgcggcgg actggccgga gcggatggcc ccacacacca tggcgccttc gatatcgcgt
     1021 acttccgttg catccccaac atgatcgtga gtgcgccgat gaacgaggag gaactgcgca
     1081 acctcatgta caccgcacaa ctgaaggacc agggaccctt cagcatccgc tacccacgcg
     1141 gcgaaggcgt gatgaccgag tggaagaccc cgttcaagga gatccccatc ggtaccggtc
     1201 ggcgtgtgcg cacgggcaat gatctcgcgg tgctcagcat cggtcatatc ggcaacatcg
     1261 ccgcgaaggg gatcgatgcg ctacaggcgg aagggtattc catcgcccac tacgacatgc
     1321 gtttcgccaa gcccatcgac gagatgctgt tgcacgaggt cttcggcaag ttcaagtatg
     1381 tgatcaccat cgaggacggt tgcatccagg gaggcatggg cagcgcggtg ctggagttca
     1441 tggccgatca tggatacagt gcccacgtga agcgcttggg gatcccggac aagtggatcg
     1501 agcacgggtc gcagaaggaa ctctacgccg aatgtggatt cgatgaggtt gcgctcgtcg
     1561 cggcggtgaa ggagatgatg ccgcagaaag gcgtgaagaa gtcgatagcc tccgcataac
     1621 ggcgcccaac gatgcgcacc gatccccgta tccgttttga gacgaaggag gagagcaacg
     1681 cccggcgcga gcgggagttc atggcgctga caccgtggga gcgcttggcg ctgtatctgc
     1741 gcgagatcac ccgcaacccc ccaacgcggg aagagcgcgg agaggcgaag ggcaacttca
     1801 tcatctggaa gcgggcggat ggagttcgag gctgaggtca aggtgttcct ttcattggcc
     1861 cagcgggagg gcctgcgcat catcatggtc ggaggcgcgg cggtgaatca ccatggctac
     1921 aaacgtcatt cagccgatat tgacctgtgg atcgaatctt caccagagaa cttcaacagg
     1981 ctcctgacgg tgctgaaggc cataggctac gaggtcgacg cgctcccgac gaaggtgttg
     2041 gccgctgagc agaacattt
//
LOCUS       DAOQOC010000532         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35867_length_2059_cov_3.108782, whole genome shotgun sequence.
ACCESSION   DAOQOC010000532 DAOQOC010000000
VERSION     DAOQOC010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35867_length_2059_cov_3.108782"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PL106_08995"
     CDS             complement(<1..1099)
                     /locus_tag="PL106_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33244.1"
                     /translation="MRRKVLALAMLLAGSTQAQDPADTNYRIGFGDTLLFNDRLHYAA
                     FGYDGPAPLFAQLWFPLIEDPATAPLSQAQLRQRTLKGPLRRVYDELLLRMDSAFIAY
                     DLRDPWDGEGPIDYSPFTEAQVKDSLFALPTRTQRPTVPQALDFPVIVYHHGSQGLSD
                     ENVRMAEHCAANGFIFLSCNFHWPLEQAPYGTPLVWEPDKSSIRTMLRFASQLANGNK
                     VFYIGHSWGAQEGWCTLHEPGLADAFVSLETTMEWKTDTAEVRDKWPQVLEAITTHSY
                     PMPILLVADTEGEPPFPLFEGVRGDLRYLDPKKPFAHEGYTSAYLMRLRGEGRFNVPD
                     AASMREQEVVYEALLAELVAFFREQMGVPGRN"
     gene            1238..1408
                     /locus_tag="PL106_09000"
     CDS             1238..1408
                     /locus_tag="PL106_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33245.1"
                     /translation="MAEPGLYAPSGTYGDATLATAAKGELLTEGLLRMILSDRLAGER
                     IGAIGEEGSRDG"
     gene            1525..1743
                     /locus_tag="PL106_09005"
     CDS             1525..1743
                     /locus_tag="PL106_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33246.1"
                     /translation="MKVPTQTVKDTNGNDQAVLVALEDWNELVGKLRHYEQLLNLKSK
                     LSSALDQVARMRSGKLKKRTLKDALRDA"
     gene            1733..>2059
                     /locus_tag="PL106_09010"
     CDS             1733..>2059
                     /locus_tag="PL106_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA33247.1"
                     /translation="MTHRVIPTPGFEKELRPLLKRHRSLAKDLLKLEKELEQRPNAGT
                     SLGHGLYKVRLAIASKGKGKSGGARVITYVVTEDREVYLLSIYDKTDYDTVDTKAMKR
                     IVTELRA"
BASE COUNT          412 a          581 c          666 g          400 t
ORIGIN      
        1 tgttccttcc gggcactccc atctgttccc gaaagaacgc caccagttca gccaacagcg
       61 cctcgtacac cacctcctgt tcccgcatgc ttgccgcatc aggcacgttg aagcgcccct
      121 ccccgcgcag ccgcatcaag tatgcactgg tgtagccttc gtgcgcaaag ggtttcttcg
      181 gatcgaggta ccgcagatcg ccgcgaacgc cttcgaacaa agggaacggt ggttcgcctt
      241 cggtatcggc caccagcagg atgggcatcg ggtagctgtg cgtggtgatg gcctccagca
      301 cttgcggcca cttgtcgcgc acctcggcgg tgtcggtctt ccattccatc gtggtctcca
      361 ggctcacaaa ggcgtcggcc aaccccggct cgtgcagcgt acaccatccc tcctgcgcgc
      421 cccagctgtg accgatgtaa aagaccttgt ttccgttggc gagttggctt gcgaagcgga
      481 gcatggtgcg gatgctggac ttgtccggct cccacacgag cggtgtgcca tagggcgctt
      541 gctccagtgg ccagtggaag ttgcacgaga ggaagatgaa gccgttcgcg gcgcagtgct
      601 cggccatgcg cacattctcg tcgctcaggc cttgcgagcc atggtggtag acgatcaccg
      661 ggaagtcgag cgcctgcggt acggtcggtc gttgggttct cgtgggcaat gcgaagaggc
      721 tgtccttcac ctgtgcttcg gtgaatggtg aatagtcgat gggcccctca ccatcccagg
      781 gatcgcgcag atcgtacgcg atgaatgcgg agtccatgcg caggagcagc tcatcgtaca
      841 cgcgtcgcag tggaccttta agcgtacgtt ggcggagctg tgcttggctc agcggtgcgg
      901 tcgctggatc ctcgataagc gggaaccaca gttgagcgaa gagcggcgct gggccatcgt
      961 agccgaaggc ggcatagtgc agcctatcat tgaagaggag cgtatccccg aagccaatgc
     1021 ggtagttcgt atcagcggga tcctgggctt gggtgctgcc agcgagcagc attgcaagag
     1081 ccaggacctt ccgtcgcatg ggggtcaatg tagtgagcgg atcttggagc aaggttgacg
     1141 caggcgctgc cacacaaggg cgacctacct tgtgcgacac accccacgat gtgcccatcc
     1201 ttccttctca tcgtcctgct aggcacattg ccgcacggtg gccgagcctg gcctgtacgc
     1261 acccagcggg acctatggcg atgccacgtt ggccaccgca gcgaagggcg aattgctcac
     1321 ggaaggtctt ctgcgcatga tcctttcgga tcgactcgct ggtgaacgca tcggtgccat
     1381 cggcgaagaa ggatcccgcg atggctgagc tggtcgtgct cggtgggacg ggtgggaccg
     1441 tggcgcaggg agcttgcccg ccgtaggcag gacgatcctg gcgatcatgg aagctggatc
     1501 ggaattgacc ttacttttga ttgcatgaaa gtcccaacac aaaccgtgaa ggacaccaac
     1561 gggaatgacc aggccgttct ggtggccttg gaagactgga acgagttggt ggggaagttg
     1621 cgccattacg agcaattgct caacctcaag agcaagctga gcagtgcact ggatcaagtg
     1681 gcgcgcatgc gttcagggaa gttgaagaag cggaccctga aggatgcgct gcgtgacgca
     1741 tagggtcatt cccactcctg gcttcgagaa ggagttgcgg ccgctgctga agcggcatcg
     1801 ttccctcgcc aaggacttat tgaagctgga gaaggaactg gagcagcgcc cgaatgcggg
     1861 caccagcctt gggcatggct tgtacaaggt gcggctggcc atagcgagca aaggcaaggg
     1921 gaagagcggc ggtgcgcggg tgatcaccta cgtcgttacc gaggaccgtg aagtgtatct
     1981 cttgagcatc tacgacaaga cagattacga cacggtggat acgaaggcca tgaagcggat
     2041 cgtgaccgaa ctgagagcc
//
LOCUS       DAOQOC010000533         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35897_length_2058_cov_3.394408, whole genome shotgun sequence.
ACCESSION   DAOQOC010000533 DAOQOC010000000
VERSION     DAOQOC010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35897_length_2058_cov_3.394408"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PL106_09015"
     CDS             <1..1175
                     /locus_tag="PL106_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA33248.1"
                     /translation="LIMGVTIGLVMSCAGIFYAPVSADLGVAQGDFGLYMTFVYGLSF
                     LMLSVAGRMMDKYSARWLLTISTAAVGVVYLAMSRFNAVWQFYVAGAIIGIALSFLLY
                     LSYPVLINRWFNARVGFFIGLCSAASGIGGVVFNPLGGHLIQAHGWRITYLIFGAIML
                     LVVMPLLGLLLRDHPSDKGLKPFGEKAEESAATKAGMDYAVAVKTPVFYGLIVFALLM
                     IAVSTLNLFMPSYVNSLGYSVEQSALVASAIMGGVTIGKVVLGYVNDRSSLAGVLIST
                     GLGIVGLLCLLMGQSAMALMVIGGFLFGWAYAGVTVETALLVRTVFGSKDYAKIFSNV
                     SIALALGGAVMAGGWGYLADRIAFQFILTAGIMLLVLSGVIGFAALRGSRKIQVQQ"
     gene            1315..>2058
                     /locus_tag="PL106_09020"
     CDS             1315..>2058
                     /locus_tag="PL106_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNA33249.1"
                     /translation="MFITRNLSFKQFRSWHTWNFLGLLAFNGIVAVAYAHKYLAVGIP
                     WLPVSVIGTAVAFYLTFKNNQAYDRMWEARKLWGGIVNSSRTWGMMVMSYVRAVDEAD
                     GERVAAIRRTLIHRQIAWLYQHRKQLLEPMTWEQVGATDRVGALARSYDRLTGLGAFP
                     TGVHVPEPATLLDPQEAQRMQGCANPAAQLINAQALQLAELREAGNLIDLRHVEMGNV
                     LKSFYDLQGGNERIKKFPFPRDYSSMSQAI"
BASE COUNT          348 a          623 c          660 g          427 t
ORIGIN      
        1 gcctgatcat gggcgtcacc atcgggctgg tgatgagctg tgccggcatc ttctatgcac
       61 cggtaagcgc ggaccttggc gtagcgcaag gcgacttcgg cctgtacatg accttcgtgt
      121 acggcttgtc gttcctgatg ctttccgtgg ctggccggat gatggacaag tacagtgcgc
      181 ggtggttgtt gaccatcagc acggccgctg tgggcgtcgt gtacctggcg atgtcgcggt
      241 tcaacgcggt gtggcaattc tacgtggccg gcgccattat cggcatcgcg ctttcgttcc
      301 tgctatacct cagctatccg gtattgatca accgctggtt caatgcccgg gtgggtttct
      361 tcatcgggct gtgcagcgcg gcttcgggca tcggcggcgt agtgttcaat cccttgggcg
      421 gtcacctgat ccaggcccat ggctggcgca tcacctacct gatcttcggg gccatcatgc
      481 tgctggtggt catgcccttg ctggggctgc tgctgcgcga tcatccctcg gacaagggct
      541 tgaagccgtt cggggagaaa gccgaggaga gcgccgccac caaggcaggg atggactatg
      601 cggtcgcggt gaagacaccg gtgttctacg gcttgattgt gttcgccttg ctgatgatcg
      661 ccgtttccac cctgaacctg ttcatgccca gctatgtcaa cagtttgggg tattccgtgg
      721 agcagtcggc cctggtggcc tcggcgatca tggggggcgt caccatcggc aaggtggtgc
      781 tggggtacgt gaacgaccgg agttcgctgg cgggcgtgct catctccacc ggcctgggca
      841 tcgtggggct gctctgcctg ttgatggggc agtcggccat ggccttgatg gtgattggcg
      901 gcttcctgtt cggctgggcc tacgccgggg tcacggtgga aacggcgctg ctggtgcgca
      961 ccgtgttcgg cagcaaggac tacgcgaaga tcttctccaa tgtctccatc gccctggcct
     1021 tgggcggcgc ggtgatggcg ggcggttggg gctacctggc ggatcggatc gcgttccagt
     1081 tcatcctcac tgctgggatc atgctcctag tcctctcggg ggtgatcggg tttgcggcgt
     1141 tgcggggaag caggaagatc caggtgcagc aataggagcg aatgaacggg cacgcctaag
     1201 agttaagatc acaacaaggt gaagtgatcg tccagaccgg tggttcggaa ccgccagcga
     1261 acccgcctcc cgccccccgc tcctgcaaca ccttccacca gataccttgc cctcatgttc
     1321 atcacccgca acctcagctt caagcaattc cgttcctggc acacgtggaa cttccttggc
     1381 ttgttggcct tcaacggcat cgtcgccgtg gcctacgcgc acaagtacct cgccgtgggc
     1441 attccctggc tgccggtatc ggtgatcggt accgccgtgg ccttctacct cacgttcaag
     1501 aacaaccagg cgtacgaccg catgtgggaa gcccgcaagc tgtggggcgg tatcgtgaac
     1561 agcagccgta cctggggcat gatggtgatg agctatgtgc gcgctgtgga cgaggctgat
     1621 ggggagcggg tggccgccat tcgccgcacc ttgatccacc ggcagatcgc ctggctgtac
     1681 cagcaccgca agcagttgct ggaaccgatg acctgggagc aggtgggcgc tacggaccgg
     1741 gtcggcgcac tggcccgctc atacgaccgg ttgaccggcc tgggcgcctt tccaaccgga
     1801 gtacacgtgc ccgaacccgc cacgctcctg gacccacagg aagcacaacg catgcagggc
     1861 tgcgccaatc cggcggcaca actgatcaac gcgcaagcgc tccaactcgc cgagctgcgc
     1921 gaagcgggca acctcatcga cctgcgccat gtggaaatgg gcaatgtgct caagtccttc
     1981 tacgacctgc agggcggcaa cgagcgcatc aagaaattcc ccttcccgcg cgactattcc
     2041 agcatgagcc aggccatc
//
LOCUS       DAOQOC010000534         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_35995_length_2055_cov_3.614000, whole genome shotgun sequence.
ACCESSION   DAOQOC010000534 DAOQOC010000000
VERSION     DAOQOC010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35995_length_2055_cov_3.614000"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="PL106_09025"
     CDS             <1..1995
                     /locus_tag="PL106_09025"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33250.1"
                     /translation="DIAAAARSVPAAQARGGTATIPVVVHVVWNIPAENVADALILQL
                     IATLNEDFNALNSDYGNVRAAFLNDRGNPQLEFCLAQTDPQGNPTTGITRTQTGETWF
                     DPENETDDMKQAPDGIAPWSPANYLNIWICDITSGASGGLITDGYAYLPYGGTAGTAI
                     DGLVVDYDYGLDAGARVATHEIGHYLGLDHPWADGGCSSDDGIDDTPVTDQPTYSCAN
                     PGLMRCNTLTQYENFMDYADCVVMFTTDQSAQMNNVLSSLRPGLLTNNACGTVIPGPC
                     VPISSNGTGLGDFIDGVQLGSISNLNSGGTSGATYNDYTAQFITQLQRGGSDTLTITS
                     GTFAPDRFAAWIDMDRDGLFEASEKLGEFTNTAPGDAQGIVFTIPLDAASDTTILRVR
                     GVYIDQGEADPVDPCFNYSRGETEDYGITIVDDGNDPCIPTSAIGTAEGDFIDGVQLE
                     GISNTGSGSTGGPVYHDYTAQNTGLARGQSYTLTLTSGAYEQDMMAAWIDLDANGTFD
                     AAELLGGAETSTAFEDVQLAFTVPADATLGTTILRARCMYPEQGEPTSADPCFNFSYG
                     ETEDYGVTILTSTGVAVPVVQAPMVWPNPAGDLLNVRLPDGSATLEVTDASGRTVLPA
                     RWSTGTSSISTTPLANGPYVLRCTTKGTTHMVRFIVRHTH"
BASE COUNT          419 a          702 c          613 g          321 t
ORIGIN      
        1 gacatcgcgg cagccgcgcg ttcggtcccg gcagcacagg cgcggggcgg tacggcgacc
       61 atcccggtag tggtacacgt ggtatggaac atcccggccg agaacgtggc tgatgcgctg
      121 atcctccagt tgatcgccac gctgaacgag gacttcaatg ccctgaacag cgactacggc
      181 aacgtgcgtg cggccttcct gaacgaccgc ggcaacccgc aactcgaatt ctgcctggca
      241 cagaccgatc cgcaaggcaa ccccaccacc ggcatcaccc gcacgcagac cggtgagacc
      301 tggttcgacc ccgagaacga gacggacgac atgaaacaag cgcccgacgg catagcgccc
      361 tggagcccgg ccaactacct gaatatttgg atctgcgaca tcaccagcgg cgcatcgggc
      421 gggctgatca ccgatggcta cgcgtacctg ccctacggcg gaacagcggg caccgcgatc
      481 gatgggctgg tcgtagacta cgactacggc ctggatgccg gtgcgcgcgt ggccacccac
      541 gagatcgggc actacttggg gctggatcac ccttgggccg acggcggatg cagcagcgac
      601 gatgggatcg atgacacacc cgtgaccgac cagcccacct attcgtgcgc caaccccggc
      661 ctgatgcgct gcaacacgtt gacccagtac gagaacttca tggactacgc cgactgcgtg
      721 gtgatgttca ccaccgatca atcggcccag atgaacaacg tgctcagcag cttgcggccc
      781 ggccttttga cgaacaacgc ctgcggcacc gtgatcccgg gtccctgcgt gcccatcagc
      841 agcaacggca ccggcctggg cgacttcatc gatggcgtgc aactcggcag catcagcaac
      901 ctgaacagcg gcggcaccag cggcgccacg tacaacgact acacagcgca attcatcacc
      961 cagttgcaac gcggcggatc cgacacgctc acgatcacct ccggcacctt tgccccggat
     1021 cgcttcgcgg cgtggatcga catggatcgg gacggccttt tcgaggcttc ggaaaaactg
     1081 ggggaattca cgaacacggc ccccggtgat gcgcagggca tcgtcttcac cataccgctc
     1141 gacgcggcat cggacaccac catactgcgc gtgcgcggcg tgtacatcga tcagggcgag
     1201 gccgatcccg tggatccctg cttcaactac agccggggcg aaaccgagga ctacggcatc
     1261 acgatcgtgg acgatggcaa cgacccctgc atccccacca gtgcgatcgg caccgctgag
     1321 ggcgacttca tcgatggcgt gcaactcgaa ggcatcagca atacgggcag tggtagcacc
     1381 ggtggccccg tctaccacga ctacaccgcc caaaacaccg ggctggcgcg tggacagagc
     1441 tataccctca cgctgacctc cggcgcatac gagcaggaca tgatggcggc ctggatcgac
     1501 ctggatgcga acggcacctt cgacgccgct gagctgctgg gcggggcgga gaccagtaca
     1561 gcgttcgagg acgtacagtt ggccttcacc gttcccgcgg acgccacgtt gggcaccacc
     1621 atcctccgtg cgcgctgcat gtaccccgaa caaggcgaac ccaccagcgc ggatccctgc
     1681 ttcaatttca gttacggcga gaccgaggac tacggcgtta ccatcctgac cagcacgggt
     1741 gtcgccgtac ctgtagtgca agcgcccatg gtgtggccca acccggccgg ggatctgctg
     1801 aacgtgcggc tccccgatgg ctccgctacg ctggaagtaa cggatgcttc cgggcgtacc
     1861 gtgttgcctg cccgatggtc gaccggaaca tcctccatca gcacgacgcc gctggccaat
     1921 ggaccctacg tcctgcgttg caccacaaaa ggcaccacac acatggtccg tttcatcgtg
     1981 cgccacacgc attgaggccg cggacgggct gcacagtccg ttcagcgggc tcggaatgcc
     2041 ctccgtcgga tcgta
//
LOCUS       DAOQOC010000535         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36235_length_2048_cov_2.236327, whole genome shotgun sequence.
ACCESSION   DAOQOC010000535 DAOQOC010000000
VERSION     DAOQOC010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36235_length_2048_cov_2.236327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2048
                     /locus_tag="PL106_09030"
     CDS             <1..>2048
                     /locus_tag="PL106_09030"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33251.1"
                     /translation="TGSTENGYFKARIGVSGTIGLIVTTNYEIYGEVGVDLTAQRSQT
                     ASEEYLTCLETTSEFTTPMTGPPDDVFIGSAVRYAYGVAKIVSRPTCDSIAREVRMAM
                     TPVQVLSSYNYTESFIRGTVMPQLQQLIGTLDPTTDPYKNAVDQLNVWQQLLDMNDDI
                     KAGAALEVNRSFNGGGNGQSYSITQTTTETRTIEYSASLDQGLGFEVGVHIGGSGITA
                     GGRMSFQNSYGSAQSASNSSTNTMTYHLEDDDASDDFHVQVRRDPVFGTYVFVLDSAL
                     SATSCEHEGGYQIDQPSLSVGVQGQNHMVLNEVPVGTGANFPLYVCNNSNVSRTYFLK
                     FRTNSNTEGGILNAFGNILNSNDNGLEIDLAAGECLNAANLVLTQPGTPIGPDYSIEL
                     YLYSLCEPAISSSITVEAHFGAGNFGGYCEPSSANGPSTGSYIDGVQLSAIDNTASGG
                     PAGAAYTDYSAQFSTPLSRNAQRVITITPGATAGARCAAWIDYDRNGIFDAEEKLGEF
                     ISTGVAQNIPFTVPGTALLGSTILRIRIAEAVNAEPVPMDPCYSYQNGETEDYAVVID
                     ANVPQDCAGVANGPALPGTACSDGNAGTGEDTWNANCQCIGVPLDCAGVPNGSTLPGT
                     PCDDGDANTAGDVYDAGCQCTGLPYDCAGIPGGNALAGSPCDDNDPFTGNDVYDGACQ
                     "
BASE COUNT          436 a          616 c          630 g          366 t
ORIGIN      
        1 cacgggaagt accgagaacg gctatttcaa ggcacgcatc ggggtgtccg gaacgatcgg
       61 cctgatcgtt accaccaact acgagatcta tggcgaagtg ggtgtggacc tcaccgctca
      121 acgatcgcag accgcatcgg aggagtacct cacctgcctg gagaccacct ccgaattcac
      181 cacgcccatg acaggtccgc cggacgatgt cttcatcggc agcgcggtcc gctatgcgta
      241 cggcgtggcg aagatcgttt cacgaccaac gtgcgattcc atcgcgcggg aagtgcgcat
      301 ggcgatgacc cccgttcagg tgctcagcag ctacaactac acggagagct tcatccgggg
      361 cacggtgatg ccccagctcc agcagttgat cggtacgctc gatcccacta ccgacccgta
      421 caagaacgcc gtggaccagc tgaacgtgtg gcaacagttg ttggacatga acgacgacat
      481 caaggccggg gcggcgctgg aggtgaaccg cagcttcaac ggcggtggca atggacagag
      541 ctattcgatc acgcagacca cgacggagac acgtacgatc gaatacagcg cctcgctgga
      601 ccaggggctc ggcttcgaag tgggtgtgca catcggtggc agcggcatca ctgcaggtgg
      661 ccggatgagc ttccagaact cctacggaag cgcgcaaagc gccagcaatt catccaccaa
      721 tacgatgacc tatcatttgg aggatgatga tgcgagtgac gacttccacg tacaggtgcg
      781 tcgcgatccg gtgttcggta cctacgtctt cgtgctggat agtgcgttat cggccaccag
      841 ctgcgagcat gaaggagggt atcagatcga ccagccctcc ctgtccgtgg gcgtacaggg
      901 ccagaaccac atggtgttga acgaagtgcc ggtgggcacg ggtgcgaact tccccttgta
      961 cgtatgtaac aacagcaacg tgtcgcgcac ctacttcctg aagttccgca cgaacagcaa
     1021 caccgaggga ggcatcctga acgccttcgg aaacatcctc aactcgaacg acaacgggtt
     1081 ggagatcgat ctcgcggcag gcgaatgcct caacgcggcg aacctggtgc tgacgcagcc
     1141 gggtaccccg atcggccctg attactcgat cgagctttat ctctactcgc tgtgcgaacc
     1201 ggccatcagt tccagcatca ccgtggaagc gcatttcggt gcgggcaact tcggtgggta
     1261 ttgcgagcct tccagcgcaa acggcccatc aacgggcagt tatatcgatg gcgtgcagct
     1321 cagtgccatc gacaatacgg cttcaggcgg cccggcgggt gcggcttaca cggactacag
     1381 cgcgcagttc agcacgcctc tctcgcgcaa tgcacaacgg gtgatcacca tcaccccggg
     1441 cgcaacagcg ggagcacgct gcgccgcatg gatcgactac gaccggaacg ggatctttga
     1501 tgcagaggag aagctgggcg agttcatcag cacgggtgtg gcacagaaca ttccgttcac
     1561 tgttcctggc acggccttgc tcggttccac catcctgcgc atccgcattg cggaagcggt
     1621 gaacgctgaa ccggtgccca tggacccgtg ctacagttat cagaacggcg aaacggagga
     1681 ctatgcggtg gtgatcgatg cgaacgttcc gcaggattgc gcgggcgtgg ccaacggccc
     1741 ggcgctgccc ggcacggcct gcagcgatgg caatgctggt accggcgagg acacctggaa
     1801 cgccaactgc cagtgcatcg gtgttccgct ggattgcgca ggcgtgccca atggcagtac
     1861 gttgccgggt acgccctgcg atgatggcga tgcgaacacg gcgggcgatg tctacgatgc
     1921 gggctgccag tgcacgggcc tgccatacga ctgtgccggc ataccgggtg gtaacgccct
     1981 ggccggatca ccctgcgatg acaatgaccc cttcaccggc aacgatgtgt acgacggtgc
     2041 ttgtcaat
//
LOCUS       DAOQOC010000536         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36407_length_2043_cov_2.329980, whole genome shotgun sequence.
ACCESSION   DAOQOC010000536 DAOQOC010000000
VERSION     DAOQOC010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36407_length_2043_cov_2.329980"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PL106_09035"
     CDS             complement(<1..902)
                     /locus_tag="PL106_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNA33252.1"
                     /translation="MSNIGVIIPERSRDIGNFMVGRLLPFHSKRMVGPFIFIDHMGPI
                     AMADHQNMDIGPHPHTGLSTLTFLFDGAVMHRDSLGTAVEITPGAVNWMTAGSGIVHS
                     ERTPERLRHVDKQMHGLQIWVALPKALEEIEPSFHHIEADALPTWEQDGARFKLIAGE
                     LMGRRSPVPVHSPLYMLEIHSETGGEMRIGAELYGEVGLYILHGSVREDSDGEVMGER
                     QILVAKDPNLCSFTMAPGTTVYLFGGEPFPEERFQGDLAPDLAGVGARLSEGQLRARV
                     VDARRANPATIMPAYRRSAGFERVG"
     gene            complement(914..1588)
                     /locus_tag="PL106_09040"
     CDS             complement(914..1588)
                     /locus_tag="PL106_09040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNA33253.1"
                     /translation="MDTDQPPGINENGRKPLRVLVFSASMRKDSLNTRLATLAAGVIE
                     KHGGKADLADMRMFDTPSFDQDRETADGPPPGAVALRDRILANDAFIISSPEYNGAMP
                     GLIKNSIDWVSRFRPQPFNEKHALLLSASPSMVGGNRALLQLRVPLEHLGTRVFPSMF
                     SLAMAHKAFDTNGGLADEKLAQRFEDNIVAFLALAEAAKNYPCIKKAWVEFPGERTDE
                     VTDRME"
     gene            1747..>2043
                     /locus_tag="PL106_09045"
     CDS             1747..>2043
                     /locus_tag="PL106_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA33254.1"
                     /translation="MQGEFVPKLFTLWRTGITREQLRKDILVGVIVGIVALPLAIAFG
                     IASGVTPEKGLITAVIAGFTISAFGGSRVQIGGPAGAFIVIVYGIVQKHGVEGLT"
BASE COUNT          338 a          638 c          667 g          400 t
ORIGIN      
        1 cccacgcgct cgaagcccgc gctgcgccgg tacgcgggca tgatcgtcgc ggggttcgca
       61 cggcgcgcgt cgacgacgcg cgctcgcagc tgtccttcgc tcaggcgcgc gccgaccccg
      121 gcgagatccg gggcgaggtc gccctggaac cgttcctccg ggaagggttc gccgccgaag
      181 aggtagaccg tggtgccggg cgccatggtg aaggaacaga gattcggatc cttcgccacc
      241 aggatctggc gctcgcccat cacctcgcca tccgagtcct cgcgcaccga tccgtgcagg
      301 atgtacaggc ccacttcgcc gtagagttcg gcaccgatgc gcatttcgcc accggtttcg
      361 ctgtggatct ccagcatgta cagcgggcta tgcacgggca cgggcgacct gcggcccatc
      421 aactcgccag cgatcaactt gaagcgcgca ccgtcctgct cccatgtcgg cagggcatcc
      481 gcttcgatgt ggtggaagct cggttcgatc tcctcgagcg ctttgggcag cgccacccag
      541 atctgcagac cgtgcatctg cttgtccacg tgccgtaagc gttctggtgt gcgttcgctg
      601 tgtacgatgc cgctccctgc tgtcatccag ttcacggcgc caggtgtgat ctccacggcg
      661 gtgcccaagc tgtcgcggtg catcacggcg ccgtcgaaga gaaaggtgag cgtgctgagg
      721 ccggtgtgcg gatgcggacc gatgtccatg ttctggtgat ccgccatcgc tatcggaccc
      781 atgtggtcga tgaagatgaa gggaccgacc atgcgtttgc tgtggaaggg cagcaggcga
      841 cccaccatga agttgccgat gtcgcgcgat cgttccggga tgatgacgcc gatgttggac
      901 atgtatgctg gtattattcc atgcggtcgg tgacctcgtc ggtgcgttca cccgggaatt
      961 ccacccaggc tttcttgatg cacgggtagt ttttcgctgc ctcagcgagg gcaaggaagg
     1021 ccacgatgtt gtcctcgaag cgttgcgcca atttctcatc ggccaggcca ccattggtgt
     1081 cgaaggcctt gtgtgccatg gccagggaga acatgctggg gaagacgcgc gtgcccagat
     1141 gctccagggg cacacgcaat tgcagcaacg ccctgttgcc acccaccatg gaaggtgagg
     1201 cggaaagcag caatgcgtgc ttctcgttga agggctgcgg ccggaagcgc gacacccagt
     1261 cgatcgagtt cttgatcagg ccgggcatcg cgccgttgta ttccggcgaa gaaatgatga
     1321 aagcatcgtt ggccaggatg cggtcgcgca gcgccaccgc gcccggaggt ggaccatcag
     1381 cggtctcgcg gtcctggtcg aacgaaggcg tgtcgaacat gcgcatgtcg gccaggtccg
     1441 ccttaccacc atgcttttcg atgacaccgg ccgccaaagt ggccaagcgt gtgttgaggg
     1501 agtcctttct catcgatgct gagaaaacga gcacccgcag gggcttcctg ccgttctcgt
     1561 tgatgccggg gggctgatcg gtgtccatgg ggcggtattg ttctgttgcg aagctatgcg
     1621 ctctgctccc cttcgggcga cacgaggctt cgccccccca cccgatgcct gctgcgccat
     1681 ggcgtgcgca gccgtgtgtt cgagccggcg tgatgcgggg atcgcgcgta tgataccttg
     1741 cgatccgtgc aaggcgagtt cgtccccaaa ctgttcacgc tctggcgtac ggggatcacg
     1801 cgcgaacaac tgcgcaagga catcctcgtc ggcgtgatcg tcggcatcgt ggccctgccg
     1861 ctggccatcg ccttcggcat cgcctcgggt gtcaccccgg agaaggggct catcacggcc
     1921 gtcatcgccg gcttcaccat cagcgccttc ggtggtagcc gcgtgcagat cggcggaccg
     1981 gcgggcgcct tcatcgtcat cgtctacggc atcgtgcaga agcacggcgt ggaagggctg
     2041 acc
//
LOCUS       DAOQOC010000537         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36423_length_2042_cov_3.514343, whole genome shotgun sequence.
ACCESSION   DAOQOC010000537 DAOQOC010000000
VERSION     DAOQOC010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36423_length_2042_cov_3.514343"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PL106_09050"
     CDS             <1..786
                     /locus_tag="PL106_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33255.1"
                     /translation="FAWLPGRLDPALAQALPSRYIVEARSILPAYLLVLLPAMARLRT
                     RGRLRAFLSQRSSRMLLTMAVVQFVLENHEVFTRPYQPAHFTRGYTWAALFLIGAPWL
                     LNEALPWIGRHRPRARTAMMVGCLMLLLSDNLAFFALNIKRELRASPPGFWLTVDQRG
                     VLDFLGTLPRDSPLVITQEEDLGYMVIVYTPLRAYRSHFFDDVDAWSRVGHQADYFAG
                     RITDPLLAGPLIVVAVQDSATFVPPGNARSLYTNAGYSVFRTE"
     gene            complement(806..1594)
                     /locus_tag="PL106_09055"
     CDS             complement(806..1594)
                     /locus_tag="PL106_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA33256.1"
                     /translation="MKDILCPTDLTPASENALRWGGAIAKAIGQSITLLHVLEKDERR
                     GDLTELKARVASEAERFASGVEVRKEFPTGDPLKGIIHGSERDHALMVAGTHGPKGLR
                     QSLFGADMLRLVRHVAVPSLVVQADSPAPGAERILLPVAGHADISHLLDATCLLARAF
                     NSEVQVFQVIRPGETPSEQLMRNKQAMIRRFRDEKIPHREVDVPSTVFSIGFAEQTIR
                     YAQESGAGIIAIMAKASDEYRWIADAEKERLLMNNARIPVLCGI"
     gene            complement(1638..>2042)
                     /locus_tag="PL106_09060"
     CDS             complement(1638..>2042)
                     /locus_tag="PL106_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33257.1"
                     /translation="MGAVTNYRPDLFRAVHAAYSTLSDPLLRIAYDARLAARDQRHAP
                     PLRPPRSNCSPSYRQRDTDEGGADIRIRSWPFIGLHLTGLVFGVLLVLTILGGITFRD
                     WPWTNLPFILPGLLVIPDAWNGLKLVFHRPGQ"
BASE COUNT          339 a          650 c          639 g          414 t
ORIGIN      
        1 ttcgcatggc tcccgggacg cctcgatccc gccctcgccc aagcactgcc ttcccgatac
       61 atcgtggagg cacgctcgat cctcccggcc tatctgcttg tgctgcttcc cgccatggcc
      121 cgattgcgca cccggggcag gctacgggcc ttcctgtcgc aacggtcgtc gcgaatgctg
      181 ctcaccatgg ccgtggtgca attcgtactg gagaaccatg aggtcttcac aaggccatac
      241 cagcccgccc atttcacccg gggctacact tgggccgcac tttttctcat cggcgcaccg
      301 tggttactga acgaggcgtt gccgtggatc gggcgtcatc ggccacgggc ccggaccgcc
      361 atgatggtgg gatgcctcat gctgctcctc tcggacaacc tggcgttctt cgcgttgaac
      421 atcaagcgcg aactacgtgc ctcgccgccg ggcttctggc tcaccgtgga tcaacgtggc
      481 gtgctcgact tcctcgggac gctgccacgc gacagccctc tggtcatcac gcaagaggag
      541 gatctgggat acatggtcat cgtgtacacg ccattgcgtg cctatcgctc gcacttcttt
      601 gatgatgtgg atgcgtggtc gcgggtgggg catcaggcgg attacttcgc gggccggata
      661 acggatccac tgctggccgg tccgctcatc gtggtcgcgg tacaggacag tgccactttt
      721 gttcctccgg ggaacgcgcg atccctttac acgaatgcgg ggtacagcgt cttccgcacg
      781 gaatgatcgg tcccgagggc gcccctcaga tcccacaaag caccgggatc cgcgcgttgt
      841 tcatcagcag gcgttccttc tccgcatcag cgatccatcg gtattcatcg ctggccttgg
      901 ccatgatggc gatgattcct gcaccggact cctgcgcgta gcggatggtc tgctcggcga
      961 agccgatgct gaaaaccgtg ctgggcacgt ccacttcgcg gtgtgggatc ttctcgtccc
     1021 tgaagcggcg gatcatcgcc tgtttgttgc gcatcaattg ctcgctgggc gtctcccccg
     1081 gtcggatcac ttggaatacc tgcacctcgg aattgaatgc acgggccagc aggcatgtgg
     1141 catcgagcag gtgactgatg tccgcgtgcc cggccacggg caacaggatc cgctcggcac
     1201 cgggcgccgg gctgtcggcc tgtaccacca gtgatggcac ggccacgtgg cgcaccaatc
     1261 tcaacatgtc ggccccgaac agcgattggc gcagcccctt cggtccgtgc gtgcccgcca
     1321 ccatgagggc gtggtcccgt tccgaaccgt ggatgatgcc cttcaacgga tcgcctgtcg
     1381 ggaattcctt ccgtacctcg acaccgctgg cgaaacgctc cgcttccgag gccacacgtg
     1441 ctttcaactc tgtgaggtcg ccccgtcttt catccttctc caacacgtgc agcagggtga
     1501 tgctctggcc gatggccttg gcgatggccc ctccccagcg gagggcgttc tccgaggcgg
     1561 gggtgaggtc ggtggggcag aggatgtcct tcattgtggg tcgccaagtt aggccgagtg
     1621 ccggtgctgg tgttccacta ttggccgggc cgatgaaaaa ccagcttcaa cccgttccat
     1681 gcgtcgggga tcaccagcag accgggcaga atgaacggga ggttggtcca tggccagtca
     1741 cggaacgtga taccgcccag tatggtcagc accagcagca caccgaagac caggccggta
     1801 aggtgaagtc cgatgaatgg ccagcttcgt atccggatgt cggcaccgcc ctcatccgta
     1861 tcgcgctggc ggtagctggg ggaacagttg gaccggggcg gtcggagtgg tggggcatgg
     1921 cgttgatcac gcgcggccag tcgtgcatcg tatgcgatcc ggagcaacgg gtcgctcaac
     1981 gtggaatagg ccgcatgcac cgccctgaac aggtcggggc ggtaattggt cacggcgccc
     2041 at
//
LOCUS       DAOQOC010000538         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36428_length_2042_cov_3.062909, whole genome shotgun sequence.
ACCESSION   DAOQOC010000538 DAOQOC010000000
VERSION     DAOQOC010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36428_length_2042_cov_3.062909"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..625
                     /locus_tag="PL106_09065"
     CDS             125..625
                     /locus_tag="PL106_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33258.1"
                     /translation="MYIGHEKSDSTFFHLPLEMGEAAVTCGTDTGYMKYTAYAKYFWP
                     LNIMTEEEFDRTFSINLGSTIEDFLQNPVSENLFDVAIFSNVFHKIQDKEIPKKMLQW
                     FTSYSTPKALLLIKVMTSNRYARENDWIYRELDLDELIACFPGRILSEDRDEDFASFL
                     IQKRSK"
     gene            668..>2042
                     /locus_tag="PL106_09070"
     CDS             668..>2042
                     /locus_tag="PL106_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNA33259.1"
                     /translation="MSIVAPNSDLHAFQASIREGVPDVLPPARPLDTSVSHAPKRKDV
                     LSPEEKKLALRNALRYFPQKHHATLAPEFARELKDHGRIYMYRFRPEGRMHARPIGDY
                     PAKSRQAAAIMLMIQNNLDPAVAQHPEELITYGGNGAVFQNWAQYRLTMHYLSEMSDE
                     QTLVMYSGHPLGLFPSHKEAPRVVVTNGMVIPNYSQPDDWERMNALGVSQYGQMTAGS
                     YMYIGPQGIVHGTTITVLNACRMLQDKGAKGKLFVTAGLGGMSGAQPKAGNIAGVVTI
                     CAEVNATAAHKRHDQGWVDEVIADVDACIDAALAWQKKGEAHSIAYLGNVVDLWERLA
                     ARRIKVDLGSDQTSLHNPWAGGYYPVGLTYEESNMLMVQDPATFKKRVEETLRRHVKA
                     VNTLAEQGMYFFDYGNAFLLESKRAGADVSPLPGRGAGGEAEFRYPSYVEDIMGPLCF
                     DHGFGPFR"
BASE COUNT          489 a          595 c          545 g          413 t
ORIGIN      
        1 atcaattaat tgaaaacctc ctcatcaaca atcccgaatt caccttggaa gataagtcta
       61 ttattgaatt cgggtttcat gatttaattc ctatcatcaa cctacatcac aactatcctt
      121 taagatgtat atcggacacg agaaaagtga ttcaacattc ttccatctcc cacttgaaat
      181 gggtgaggcg gccgttacct gtggcaccga tacgggttat atgaagtata ccgcctatgc
      241 caagtatttt tggccgctaa atatcatgac cgaagaagaa tttgatcgga cattcagcat
      301 taacctaggt tctaccatag aagatttctt acaaaaccca gtttctgaga atcttttcga
      361 cgttgccata ttttcgaacg tgtttcataa aattcaagat aaagagatac caaagaagat
      421 gttacaatgg ttcacatcct attcaacacc aaaggctcta ttgctcatca aggtaatgac
      481 gtctaaccga tatgcccgtg aaaatgattg gatatatcga gaattggact tggacgaatt
      541 gattgcctgt tttcccggaa gaatcttatc ggaagataga gatgaagatt ttgcttcatt
      601 tctgatacag aaacggtcca aatagaatac tcaacgccaa attggcggcg cccccgatct
      661 tcgcactatg tcaatcgttg ccccaaattc cgacctccac gccttccaag cctccatccg
      721 cgaaggcgtt cccgatgtgc tgccccccgc ccgcccgctc gatacgagcg tgagccatgc
      781 ccccaagcgc aaggacgtcc tttcacccga ggagaagaag ctcgccttgc gcaacgccct
      841 gcgctacttc ccgcaaaaac accacgccac cctcgctccc gagttcgccc gggaactgaa
      901 ggaccacggt cgcatctaca tgtaccgctt ccgccccgaa ggccggatgc acgcgcggcc
      961 catcggcgac tacccggcca agagcaggca ggccgccgcc atcatgttga tgatccagaa
     1021 caacctggac cccgccgtgg cgcagcaccc cgaggagctg atcacctacg gtggcaacgg
     1081 cgcggtgttc cagaactggg cgcagtaccg gctcaccatg cactacctca gcgaaatgag
     1141 cgacgagcag acgctggtga tgtacagcgg ccatccgttg gggctcttcc ccagccacaa
     1201 ggaagcgccg cgcgtggtgg tcaccaacgg catggtgatc cccaactaca gccagcccga
     1261 tgattgggag cgcatgaacg ccttgggcgt ttcgcagtac ggccagatga cggccggcag
     1321 ctacatgtac atcggtccgc aagggatcgt gcatggtacc acgatcacgg tattgaacgc
     1381 ctgccgcatg ctccaggaca agggtgcgaa aggcaagctc tttgtcacgg cagggctcgg
     1441 cggcatgagc ggcgcccagc ccaaggcggg caacatcgcg ggcgtggtaa cgatctgtgc
     1501 cgaggtgaac gccaccgccg cgcacaaacg ccacgatcaa ggctgggtgg atgaagtgat
     1561 cgccgatgtg gatgcctgca tcgatgccgc gctggcttgg cagaagaagg gcgaagcgca
     1621 cagcatcgcg tacttgggca acgtggtgga cctgtgggaa cggttggccg cgcgcaggat
     1681 caaggtggac ctcggcagcg accagaccag tttgcacaat ccgtgggccg gtggctacta
     1741 ccccgtgggg cttacgtatg aggagagcaa catgcttatg gtgcaggacc ccgccacctt
     1801 caaaaagcgc gtggaggaaa ccctgcgccg gcacgtgaag gccgtgaaca ctctggccga
     1861 acagggcatg tacttcttcg actacggcaa cgccttcctg ctggaaagca agcgtgccgg
     1921 tgccgatgtc tcccctctcc ccgggagagg ggccgggggt gaggccgaat tccgctaccc
     1981 cagttatgtg gaggacatca tggggcccct gtgcttcgac cacggcttcg ggcccttccg
     2041 tt
//
LOCUS       DAOQOC010000539         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36544_length_2039_cov_2.662298, whole genome shotgun sequence.
ACCESSION   DAOQOC010000539 DAOQOC010000000
VERSION     DAOQOC010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36544_length_2039_cov_2.662298"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1346
                     /locus_tag="PL106_09075"
     CDS             123..1346
                     /locus_tag="PL106_09075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNA33260.1"
                     /translation="MTRLDRITERLNLKEFQLRALLELTKAINGNQQRQELIDLYVSI
                     MRDDLGITRLMLFEHAADWGCAHAYGADEAQTHMDVRPLIELYKDIQFIGSSEGPVIG
                     GFDIAVPVYHQERPIALLLIGDIDEEERRVSPAVKHMNFIQTLTNLIAVALENKRLGE
                     LALLQERDKRELELAAEMQHMLIPRELPDDGAVRAAAWYKPHRQVGGDYYDLVRFSDH
                     ELLVCVADVSGKGMAAALLASNFQATLRAMLTRDHERLDVMVQELNRLVNERARGERF
                     ITLFIGVADTRKGVLRYVNAGHNAPILLRQGGLQELTDGSIALGMLDQLPFVRTGEAA
                     IGGGTLLCYTDGLVEQEDAYGAAFGTAPLERALDQFAGAGPGAVNDALIATFEAHRSG
                     LPYLDDIALLTCRFA"
     gene            complement(1304..>2039)
                     /locus_tag="PL106_09080"
     CDS             complement(1304..>2039)
                     /locus_tag="PL106_09080"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA33261.1"
                     /translation="LLLGMIFSYTRAAWVGVVAALGVLVVLKLRVPGWIVFGTALVAG
                     GVYLVNRDQITIALERNRQESSDDLAKHVSSISNISSDASNLERINRWNSALRMFEER
                     PWTGWGPGTYMFQYAPFQAAADRTIISTNFGTGGNAHSEFLGPLSEQGLPGMLIVVAL
                     IGVTTWTALRLWLRLPPGAERGMVGAAFLGLVSYYVHGTLNNFLDLDKASVPFWGFTA
                     MLVVMDLRNPPAAQAKRQVSSAISSK"
BASE COUNT          372 a          650 c          682 g          335 t
ORIGIN      
        1 caacaatgcg gagcccggaa aaacatacct cggatctcca gcacgcgagg tgcgcgacat
       61 gctgcgtgag atctccctgc gcgcccgcct gcccgagatc gcccggaaga tcggtctctg
      121 acatgacacg cctggaccgc atcaccgagc gattgaacct gaaggaattc cagttgcgcg
      181 cgctgctgga actcaccaag gccatcaacg gcaaccagca gcgccaggag ctgatcgacc
      241 tgtacgtgag catcatgcgc gacgacctgg gcatcacccg gctgatgctc ttcgagcacg
      301 ctgcggactg gggctgtgcg cacgcttacg gggcggacga ggcgcagacc cacatggacg
      361 tgcggccgct gatcgagctg tacaaggaca tccagttcat cggcagcagc gagggtcccg
      421 tgatcggtgg cttcgacata gccgtaccgg tgtaccacca ggagcggccc atcgcgctgt
      481 tgctcatcgg cgacatcgac gaggaggagc ggcgtgtgag ccccgcggtg aagcacatga
      541 acttcatcca gacgctcacc aacctgatcg cggtggcgct ggagaacaag cgcctggggg
      601 aactggccct gttgcaggag cgcgacaagc gcgagctgga gctggccgcc gagatgcagc
      661 acatgctgat cccacgcgaa ctgccggacg atggtgcggt ccgggccgcg gcctggtaca
      721 agccccaccg gcaggtgggc ggcgactact acgacctggt gcgcttcagc gatcacgaat
      781 tgttggtatg tgtggcggac gtgagcggca aaggcatggc cgccgcgctg ctcgcgtcga
      841 acttccaggc gaccctgcgt gccatgctca cgcgtgacca cgaacggttg gacgtgatgg
      901 tgcaggagtt gaaccggctg gtgaacgaac gggcgcgtgg cgagcgtttc atcaccctgt
      961 tcatcggggt ggccgatacg cggaaaggcg tattgcgcta tgtgaacgcg gggcacaacg
     1021 ccccgatcct gctgcgccag ggcgggctcc aggaactgac cgacggcagc atcgccctgg
     1081 gcatgctgga ccagctgccc ttcgtgcgca cgggtgaggc ggcgatcggc ggtggtaccc
     1141 tgctgtgcta taccgatggt ctggtggagc aggaggacgc gtacggcgcg gctttcggca
     1201 ccgccccgct ggagcgtgcg ctcgaccagt tcgcgggggc cggtcccggc gcggtgaacg
     1261 atgcgctcat cgccaccttc gaggcgcacc ggtcgggtct tccttatttg gacgatatcg
     1321 cgctgctcac ctgccgcttc gcctgagcgg cgggcgggtt gcgcaggtcc atcaccacga
     1381 gcatggccgt gaatccccag aagggaacgc tggccttgtc caggtccagg aagttgttga
     1441 gcgtaccgtg aacgtagtag ctgaccaggc ccaggaaggc cgcaccgacc atgccgcgtt
     1501 cggcgccggg cggcaggcgc agccacaggc gcagggccgt ccaggtggtc acaccgatga
     1561 gtgctaccac gatgagcatg cccggcagcc cctgctcgct gagcggaccg aggaactcgc
     1621 tgtgcgcgtt gccgcccgtt ccgaaattgg tgctgatgat ggtgcggtcc gcagcggcct
     1681 ggaagggcgc gtactggaac atgtaggtcc ccggtcccca gccggtccac ggccgttcct
     1741 cgaacatgcg cagggcggag ttccaccggt tgatgcgttc gaggttgctg gcatccgaac
     1801 tgatgttgct gatggaactc acatgcttgg ccaggtcgtc gctgctctcc tgtcggttgc
     1861 gttccagtgc gatggtgatc tgatcacggt tcaccagata aacgcctccc gccaccagtg
     1921 cggtaccgaa cacgatccat ccgggcacgc gcagcttcag caccacgagc acgcccaaag
     1981 cggccaccac ccccacccag gcggcccggg tgtacgagaa gatcatgccc aacagcagc
//
LOCUS       DAOQOC010000540         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36649_length_2036_cov_2.242302, whole genome shotgun sequence.
ACCESSION   DAOQOC010000540 DAOQOC010000000
VERSION     DAOQOC010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36649_length_2036_cov_2.242302"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PL106_09085"
     CDS             complement(<1..294)
                     /locus_tag="PL106_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNA33262.1"
                     /translation="MNKHLNIGLLIAGAALLSGCQFEKSGATGWNYNDSKNGGFEKAP
                     FVEQENGPGLILVEGGQFTMGRVTDDLTHDWDNIPRTVTVSSFYMDEVEVTNFY"
     gene            complement(345..1307)
                     /locus_tag="PL106_09090"
     CDS             complement(345..1307)
                     /locus_tag="PL106_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA33263.1"
                     /translation="MGRAALWLALLVAAPLSAWGQDPQFTQFYANPLYLNPAFAGTAR
                     CPRVVLNYRNQWPALSGTFVTTSASYDQDVRGILGGLGLLVTNDQAGKGTLTTTTISG
                     IYSYTQAVSRKFSIKAGFQATYFQKSLDWNKLTFGDMIDPNRGFIYQTNDVPRGGKVG
                     NADFSAGILGYSDIFFVGFAAHHLTQPNESLIVGTSKMPMKLTAHAGAAIPLGQRGKY
                     GAAKTKIAPNVLYMQQAAFRQLNLGLYVDHGPIIAGVWYRSKDAFIALIGFHTDRLKF
                     GYSYDVTTSKLTTATAGSHEVSLTLQFNCKKKRRRVRTVPCPTF"
     gene            1653..>2036
                     /locus_tag="PL106_09095"
     CDS             1653..>2036
                     /locus_tag="PL106_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33264.1"
                     /translation="MRHRSLTSLFVLFALSLGAQGQVAAPKDRQARSRPEQEPARQVV
                     AAPAKEALPTDGKEKVGPTDWMNALMRDGEREGLPYIQEVRRLGPGATGATAHLSGTR
                     YVPLSAEEIALLPGLGQPGQEPEVAV"
BASE COUNT          359 a          605 c          674 g          398 t
ORIGIN      
        1 gtagaaattg gtcacctcca cctcatccat gtagaacgag gacacggtca cggtacgcgg
       61 gatgttgtcc caatcgtgcg taaggtcgtc cgtaacacgg cccatggtga actggcctcc
      121 ctccacgagg atgagtccgg ggccgttctc ctgttccacg aagggggctt tttcgaagcc
      181 gccgttcttg gagtcgttgt agttccaccc ggtggcgccg ctcttttcga actggcaacc
      241 gctcagcagc gccgcgccgg cgatgaggag cccgatattc aggtgtttgt tcatggcctt
      301 gaggttgggg ttccaccgcc cggcggggaa ccaaggaagc ttggttagaa cgtggggcag
      361 ggcacagtgc gtacccggcg gcgttttttc ttgcagttga attggagcgt gaggctcact
      421 tcgtgcgatc cggccgtggc cgtggtgagc ttgctggtcg taacgtcgta gctgtaaccg
      481 aatttcaggc gatcggtatg gaagccgatc agggcgatga aggcgtcctt ggagcggtac
      541 cacaccccgg cgatgattgg gccgtgatcc acgtagaggc ccaggttcaa ctggcggaag
      601 gccgcctgct gcatgtacag cacattgggc gcgatcttgg ttttggccgc accgtatttg
      661 ccacgctggc cgagggggat ggcggcaccc gcatgggcgg tgagtttcat gggcattttg
      721 ctggtgccca caatgagcga ttcgttcggc tgtgtgaggt ggtgcgcggc gaagcccacg
      781 aagaaaatat cggagtaccc caggatcccc gccgagaagt cggcgttgcc caccttgccg
      841 ccgcgcggaa catcgttcgt ctggtagatg aagccgcggt tcggatcgat catgtcgccg
      901 aaggtgagct tgttccaatc cagcgatttc tggaaatagg tggcctggaa gccggctttg
      961 atggagaatt tgcggctgac ggcttgcgtg tacgaataga tgccgctgat ggtcgtggtg
     1021 gtgagcgtgc ccttgccggc ctgatcgttg gtgaccagca ggcccaagcc gcccaggatg
     1081 ccgcgcacgt cctgatcgta gctggcgctg gtggtgacga aggtgcccga aagcgcgggc
     1141 cactggttgc ggtaattcag caccacgcgc gggcagcgcg ccgtgcccgc gaaggcgggg
     1201 ttcaggtaca gcggattggc gtagaactgg gtgaactgcg gatcctgccc ccacgcggag
     1261 agcggtgcgg ccaccaacaa tgccagccaa agggcggcgc gccccatggt ccttctcatc
     1321 atgctctctt ccgtttcatc acccggtaca agcgccaagt atacggaccg tccccggtca
     1381 gggttcgtgc cacgcccccc gcaataaggg gaagccgcgg acgttggtcg tgtgttccga
     1441 aacacgaggg ccgaatgtag tggttccggc gatacactgt tgaaaacctg ttgaaagtgt
     1501 tcttcacgcc tgcccgttgc tttcctcccc gtgaagggcc atccgggcac gtcgcacgca
     1561 ggtttccaat gcgcaggaac acgttcgtaa cgggttcgcc caccctgttc atagtttcgt
     1621 ccacttccat gcacagcaac ccggcccccg gcatgcgcca ccgatccctg accagcctgt
     1681 tcgttctttt tgccctttcc cttggagccc aagggcaggt ggccgcgccg aaggaccggc
     1741 aagcgaggtc ccggcccgag caggaacccg ctcggcaggt ggttgctgcc ccggccaagg
     1801 aggccctgcc gaccgacgga aaggagaaag tcggtcctac cgactggatg aacgcattga
     1861 tgcgggacgg ggaacgggag ggcctgccgt acatccagga agtgcggcgc ctgggaccgg
     1921 gagccacggg agccacggcg catctgtccg gaacgcgcta cgtgccgctc tccgccgagg
     1981 agatcgccct gttgcccggc ctgggccagc ccggccagga gcccgaagtg gccgtt
//
LOCUS       DAOQOC010000541         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36700_length_2034_cov_3.056594, whole genome shotgun sequence.
ACCESSION   DAOQOC010000541 DAOQOC010000000
VERSION     DAOQOC010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36700_length_2034_cov_3.056594"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PL106_09100"
     CDS             complement(<1..312)
                     /locus_tag="PL106_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33265.1"
                     /translation="MPDAVLRPADSDQAERLHAFAHDIKNRLHGLWEALRMSNDSSIE
                     GADKAGIIALAERGYFSAQRELEQLLDDLAVDRDVRAEHKRIDIVSCLNTALHHEAYR
                     LK"
     gene            complement(305..673)
                     /locus_tag="PL106_09105"
     CDS             complement(305..673)
                     /locus_tag="PL106_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA33266.1"
                     /translation="MSKVLIIEDEAIISFGYRLQLEQMGFEVVGTARSSDEAQAILSA
                     VRPDVIVMDVYIQGDINGLELAQRIHQKDTIPVIFMTASSDPTIVEGIRALEDCHYLL
                     KPVDTDALTSLLHSTARANA"
     gene            complement(697..1101)
                     /locus_tag="PL106_09110"
     CDS             complement(697..1101)
                     /locus_tag="PL106_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA33267.1"
                     /translation="MRETIEHVLLVDDEDDYHFITRIVLKKAGFNGKFECFYSADEAM
                     ARLRTDSTKPDLILLDINMPGYTGFDMLRTMEAEGLLPNGTSLVVMCSSSNRPMDIEQ
                     ARSFRSVDDYIEKSFDLGQFERLTEVFKQKQA"
     gene            complement(1164..>2034)
                     /locus_tag="PL106_09115"
     CDS             complement(1164..>2034)
                     /locus_tag="PL106_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNA33268.1"
                     /translation="DPLVRRVLGSLLPPTVLQRLEGLSRPRPTSEDRSPTRSLNDHIV
                     IIGYGLNGQNVALAAQSARIRCAVIEEDVDLAQKARARGMHAVIGDASNEHVLEQAHV
                     ENARVVVIAIADAETTRRITATVRAHTSAYIIVRTRYVREIGANLDAGANEVIPEEFE
                     TSIEIFYRVLRKYLVPERSIQELVAHIRGDHYGMLRGTPSTGTIPATMTGEAGEMGIA
                     TVPVDLGRSRIAGRTLAHVDLHGRFGITLLAIRRKNRVITRPEAEERIQQDDLLYLLG
                     TPANIRRLDHELR"
BASE COUNT          343 a          630 c          612 g          449 t
ORIGIN      
        1 cttcaaccgg tacgcctcgt ggtgcagcgc cgtgttgagg cagctgacga tgtcgatccg
       61 tttgtgctcc gccctgacat cacggtccac ggcgaggtcg tcaagcaatt gctccagttc
      121 gcgctgcgca ctgaaatagc cgcgctcggc gagggcgatg atccctgcct tgtccgcgcc
      181 ttcgatggaa ctgtcgttgg acatccgcag cgcctcccat aaaccgtgca ggcggttctt
      241 gatgtcgtgc gcgaaggcat gcagccgctc ggcctggtcg ctgtccgcag ggcgcagcac
      301 cgcgtcaggc attggcgcgc gccgtggagt gcaataaact ggtcagggcg tcggtgtcca
      361 ccggcttcaa caggtagtgg cagtcctcca gggcgcggat gccctccacg atggtgggat
      421 cgctgctggc cgtcatgaag atcactggaa tggtgtcctt ctggtggatg cgctgcgcga
      481 gttcgaggcc gttgatgtcg ccttggatat atacgtccat cacgatcacg tcgggccgca
      541 cggcggagag gatcgcttgg gcctcgtcgc tgctgcgggc ggtcccgacg acctcgaagc
      601 ccatctgttc caactggagc cggtaaccga aggagatgat cgcttcgtcc tcgatgatca
      661 gtactttgct catggcgctg tggtcgttga gttggctcag gcctgtttct gtttgaagac
      721 ttccgtcaac cgctcgaatt gtcccagatc gaatgatttc tcgatgtaat cgtccacgct
      781 gcggaagctg cgtgcctgct cgatatccat cgggcggttg ctgctgctgc acatcaccac
      841 caggcttgtg ccattgggaa gcagtccctc ggcttccatg gtgcgcaaca tgtcgaatcc
      901 cgtatatccg ggcatattga tgtccaacag gatcaggtca ggcttggtgc tgtccgtacg
      961 gagccgggcc atcgcctcat cagcgctgta aaagcactcg aacttcccgt tgaaccccgc
     1021 ctttttcagt acgatccgcg tgatgaaatg atagtcatcc tcatcatcca ccaacaggac
     1081 atgctcgatc gtctccctca tttcacactg gaattggggc gttcttacgg gacaggcggg
     1141 cggaatgttc ccccgcgcgt acctcaacgg agctcgtggt ccagtcgccg gatgttggcc
     1201 ggtgtgccca gcaggtacag caggtcatcc tgctggatgc gctcttcggc ttcgggccgg
     1261 gtgatcaccc tgttcttgcg tcggatggcc agcagggtga tcccgaagcg cccgtgaagg
     1321 tccacgtgtg caagcgtcct gcccgcgatg cgcgaacgtc ccaggtccac tggaaccgtg
     1381 gcgataccca tttctcccgc ttcaccggtc attgttgcag gtattgttcc ggtggaaggg
     1441 gttccacgca acatgccgta atgatcacca cggatatgtg ccaccagttc ctggatcgat
     1501 cgctcaggaa ccaggtactt gcgcaggaca cgatagaaga tctcgatgct cgtctcgaac
     1561 tcctcaggga tcacttcgtt ggccccggca tccaggttcg caccgatctc ccgaacgtac
     1621 cgcgtccgta cgatgatata cgcgctcgta tgcgcccgca ccgtggccgt gatgcgtcgg
     1681 gtggtttccg catcggcgat ggcgatcacc accacccggg cgttttccac atgggcttgc
     1741 tccagcacat gctcgttgct cgcatcgccg atcaccgcat gcataccgcg ggcacgcgcc
     1801 ttttgggcca ggtccacgtc ctcttcgatc acggcgcaac ggatgcgcgc gctttgcgcc
     1861 gccagtgcca cattctggcc gttgagcccg tatccgatga tgacgatgtg atcgttgagc
     1921 gatcgggtcg ggcttctgtc ctcgctcgtg ggccgcgggc ggctgagccc ttccaagcgt
     1981 tgcagcaccg tgggtggtaa cagggacccc agtacgcggc ggaccaacgg gtcc
//
LOCUS       DAOQOC010000542         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36709_length_2034_cov_2.494694, whole genome shotgun sequence.
ACCESSION   DAOQOC010000542 DAOQOC010000000
VERSION     DAOQOC010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36709_length_2034_cov_2.494694"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="PL106_09120"
     CDS             complement(<1..2016)
                     /locus_tag="PL106_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNA33269.1"
                     /translation="MLWPLPVLLALGVRRLGRWRWTALLVIIASLSFFTVHEINRQNF
                     KRADSDRAALAESAVAREDPVVEWLFRETRTAIAHDPSAMELLSDTGSIAPTMLDTRL
                     RLPFFQGAWDAYDVRLHLFGTNGRARGSTSRGAVPVLNELRERFGQGVPVEGDTLLRN
                     TRRPMERALYIGQAGVGTGGTLVVELLPRALPEGIGFPELLMAGDRAMDRRTGRFARA
                     RYERGSLVESSGAFAFPVRWEKPVPVDGLELETGHHRLYVSGDPGNTVVVIATRMPGW
                     RDHLTTFSYILILYALLAAGYLFIRNIFAGRWPHVLGVANKLRAGILLLACIAVVLFA
                     VGSQRLLDDDISARTKEQLDERSRSALAELRRRTHAEPRITPAMFPDIDRWIDRAATV
                     LLTDITAWTPEGGLMATSREQVFVNGLLGRQMDPDAYEALALGHRSVYTHTERIGEAE
                     FLATYRPLLNDDGEVLAYLEVPYYARQSEVDDARAAGYVTIVNLFVLLLLIGTAAATL
                     IAAWTTRPLSVLKRGLERIQLGARNEPLPYQGDDELGELVRVYNRKVEELRISAEKLA
                     RSERESAWKEMARQVAHEIKNPLTPMKLNIQHFQHTWRPDSPDAKERLDRFSDHLVDQ
                     IDVLSRIANEFSHFAQMPPAHPTALDLGEVADTVVQLFAHSPGCTVRS"
BASE COUNT          383 a          660 c          634 g          357 t
ORIGIN      
        1 cgatcggacc gtgcatccgg gcgagtgggc gaacagctga acgacggtat cggccacttc
       61 tccgaggtcg agtgcggtcg gatgcgccgg gggcatctgc gcgaagtggc tgaactcatt
      121 ggcgatccgg ctgagcacat cgatctggtc taccagatgg tcgctgaagc ggtccaaccg
      181 ctccttcgca tcgggtgaat ccgggcgcca cgtgtgctgg aaatgctgga tgttcagctt
      241 catgggcgtc agtggattct tgatctcgtg cgccacctgt cgcgccatct ccttccatgc
      301 gctctcacgc tcgctgcggg ccagcttctc ggcgctgatg cgcaactcct ccactttccg
      361 gttgtacaca cgcaccagtt caccgagttc atcatcgccc tgatagggca gcggttcgtt
      421 gcgggccccc agttggatcc gctccagacc gcgcttgagc acgctcaggg ggcgggtggt
      481 ccacgccgcg atgagggttg cggcggcggt cccgatcagg agcaggagta cgaagaggtt
      541 cacgatggtc acgtaacccg ccgcacgcgc atcgtccacc tcgctctgcc gggcgtaata
      601 cggtacctcc aggtaggcca gcacctcccc atcatcgttc aacaagggcc ggtacgtggc
      661 caggaactcc gcctcaccga tccgttccgt atgcgtgtac acgctgcggt gcccgagtgc
      721 caaagcctcg tacgcatcgg gatccatttg gcggcccagc aagccgttca cgaagacctg
      781 ctcacgtgaa gtggccataa gccccccttc cggggtccac gccgtgatgt cggtaagcag
      841 gaccgtggcc gcacgatcga tccaccggtc gatgtcaggg aacatggccg gggtgatgcg
      901 cggttccgcg tgcgtgcgtc tccggagctc ggccagggcg ctgcgcgacc gttcatcgag
      961 ctgctccttg gtgcgcgcgc tgatgtcatc atcgagcagc cgttgtgatc ccacggcgaa
     1021 cagcaccacc gcgatgcagg ccagcaagag gatgccagca cgcagcttgt tcgccacccc
     1081 gaggacgtgc ggccatcttc cggcaaaaat gttccgtatg aacaggtatc cagcggcgag
     1141 cagggcatag aggatgagga tataggagaa ggtggtgagg tgatccctcc aaccgggcat
     1201 gcgcgtggcg atgaccacta ccgtgttccc aggatcacca ctgacgtaga gccgatggtg
     1261 gcccgtctcc agttccagac catcgaccgg caccggcttt tcccaacgca cggggaaagc
     1321 gaaggcaccg ctgctctcca cgagcgatcc gcgttcatac cgggcacgcg cgaatcggcc
     1381 cgtgcggcga tccatggcgc gatcaccggc catgagcaat tcggggaacc cgatgccctc
     1441 gggcagtgcg cgcggcagca actccaccac cagggtgcca ccggttccca cccccgcctg
     1501 gccgatgtac agggcccgtt ccatgggcct ccgcgtgttg cgcaacaagg tatcgccttc
     1561 cacaggaaca ccctggccga agcgctcacg caattcgttc agaacgggga ccgcgccccg
     1621 tgaggtactg ccgcgcgccc gtccattcgt accgaagaga tgcaaccgca cgtcgtacgc
     1681 gtcccaggct ccttgaaaaa agggtagacg gagccgggta tcgagcatgg tgggtgcaat
     1741 gctgccggtg tcggacaata attccatcgc ggaaggatca tgggcgatgg ccgttctcgt
     1801 ctcccggaag agccattcca ccacggggtc ctccctcgca accgccgatt cagcgagggc
     1861 ggccctgtcg ctatcggccc gtttgaagtt ctgccggttg atctcatgca ccgtgaagaa
     1921 actgagtgaa gcgatgatga cgagcaatgc cgtccagcgc cagcggccga gccgtcgaac
     1981 acccagcgcg agcagcacgg gcaatggcca gagcacggga acggaatcat acat
//
LOCUS       DAOQOC010000543         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36728_length_2033_cov_3.556623, whole genome shotgun sequence.
ACCESSION   DAOQOC010000543 DAOQOC010000000
VERSION     DAOQOC010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36728_length_2033_cov_3.556623"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PL106_09125"
     CDS             complement(<1..1379)
                     /locus_tag="PL106_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33270.1"
                     /translation="MTTNIEHQRVPLSPNQKSRRINQDMDLYGTFAEIGAGQETVRHF
                     FRAGGASMTIAKAMSAYDKDFSNAIYGPEPGNRFVCESRLKNMLRHEYGLMLERLSRK
                     DHPTRKFFTFANTVATSTFERPHGGHGWLGVRFQTAPDRPTSDVIVHVRLHDPDISLQ
                     QENIGIMGVNLIHACFFHHKDPQEIMTALYDNVSRHVVEIDMIQMNGPDFSQVDNRLL
                     SLQLVKRGYTDAVIFGPDGQNLQASEVLYKKNILAIRGSFRPVTKVNIDMIMNGYNMF
                     IKENRVDRQKLQVLFEITLNNLQADTGDVDEKDFIDRADILCSLGQTVLISNYQEYYK
                     LVEYFSRFTKARLGLIMGVNNLIEVFEEKYYRNLNGGMLEAFGILFTRDLKVYVYPSK
                     PTADVETMTLENMPVHPRLRPLYDYLVSNKRMVDIENYDPSVLPIFSQAALRMIRDGE
                     PGWESMVPP"
     gene            1538..>2033
                     /locus_tag="PL106_09130"
     CDS             1538..>2033
                     /locus_tag="PL106_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HNA33271.1"
                     /translation="MKILVLIAQVPDTTSRIAFKDGGTAFDGTGVTWIVNPYDEWYAL
                     VRALELKEAAGAGSITTITVGGADAEATIRKALAIGADDAVRVDAQPADAYGVATQVA
                     AYAKDKGFELILAGKETIDHNGGQVGAMVAELLDLPYVALASKLDVAAGKATIERDVN
                     GGGEV"
BASE COUNT          366 a          580 c          672 g          415 t
ORIGIN      
        1 tacggcggca ccatgctctc ccagcccggt tcaccgtcgc ggatcatgcg cagcgcggcc
       61 tggctgaaga tggggagcac cgaggggtcg tagttctcga tgtccaccat gcgcttgttg
      121 ctcaccagat agtcgtacaa gggccgcagg cgcggatgta ccggcatgtt ctccagcgtc
      181 atggtctcca cgtcggccgt gggtttgctc gggtacacgt acaccttcag gtcgcgggtg
      241 aagaggatgc cgaaggcctc gagcatgccg ccgttcaggt tgcggtagta tttttcctcg
      301 aaaacctcga tcaggttgtt cacgcccatg atcaggccga gccgggcctt ggtgaagcgg
      361 ctgaagtact ccaccagctt gtaatactcc tggtaattgc tgatcagtac ggtctgcccc
      421 agggagcaca ggatgtcggc ccggtcgatg aagtccttct cgtccacgtc gccggtatcg
      481 gcctgcaggt tgttcaacgt gatctcgaag agcacctgga gcttctgacg gtcaaccctg
      541 ttctccttga tgaacatgtt gtacccgttc atgatcatgt cgatgttgac cttggtcacg
      601 ggccggaagc tgccgcggat ggcgaggatg ttcttcttgt agagcacctc gctggcctgc
      661 aggttctggc catcgggacc gaagatcacc gcgtcggtgt agccgcgctt caccagttgg
      721 aggctcaaca agcggttgtc cacctggctg aagtcgggcc cgttcatctg gatcatgtcg
      781 atctccacca cgtgccggct cacgttgtcg tacagcgcgg tcatgatctc ctgcgggtcc
      841 ttgtggtgga agaagcaggc gtggatcagg ttcacgccca tgataccgat gttctcctgc
      901 tgcaaggaga tgtccgggtc gtgcaagcgc acgtggacga tgacatcgct ggtcggtcga
      961 tcgggcgcgg tttggaaccg gacaccgagc catccgtgcc cgccatgggg ccgctcgaag
     1021 gtgctggtgg ccaccgtatt ggcgaaggtg aagaacttgc gggtggggtg gtccttgcgg
     1081 ctgaggcgtt cgagcatgag gccgtactcg tggcgcaaca tgttcttcaa ccggctctcg
     1141 cacacgaaac ggttaccggg ttcgggcccg tagatcgcat tgctgaaatc cttgtcgtat
     1201 gcgctcatcg ccttggcgat ggtcatgctg gccccgcccg cccggaagaa gtgacgcacc
     1261 gtttcctgcc cggcaccgat ctcggcgaac gtgccataga ggtccatgtc ctggttgatc
     1321 cgccgggact tctggttcgg actcaacggt acgcgttggt gctcaatatt ggtcgtcatc
     1381 gcaagggcat ccgtgaacgt ggcaaatgta gggtggtccg cggcccggag ccgacgtggc
     1441 ggggaagtgc ggcacccggg atcgtccgtt catgaacgat gggaccgacc ggaaccgtcg
     1501 gcctgttaag tttgcggccc cattcaaaaa tggaagcatg aagatcctgg ttctcatcgc
     1561 gcaagtaccc gataccactt cgcgtatcgc gttcaaggat ggcggcacgg ccttcgacgg
     1621 caccggcgtc acctggatcg tgaatcccta cgatgaatgg tatgcgctgg tgcgcgcact
     1681 ggaactgaag gaagcggccg gagcggggag tatcaccacg atcacggtgg ggggtgctga
     1741 tgcggaggcc accatccgca aggccctggc catcggtgcc gacgatgcgg tgcgtgtgga
     1801 tgcgcagccc gctgatgcgt atggcgtggc gacccaagtg gcggcatacg ccaaggacaa
     1861 gggcttcgag ctgatccttg cgggcaagga gaccatcgac cacaacgggg gacaggtagg
     1921 ggccatggtg gccgaactgc tcgacctgcc gtacgtggca ctggccagca agctcgatgt
     1981 tgcggccggc aaggcgacca tcgaacgcga cgtgaacggc gggggggagg tgg
//
LOCUS       DAOQOC010000544         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36758_length_2032_cov_4.978250, whole genome shotgun sequence.
ACCESSION   DAOQOC010000544 DAOQOC010000000
VERSION     DAOQOC010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36758_length_2032_cov_4.978250"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PL106_09135"
     CDS             <1..1030
                     /locus_tag="PL106_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNA33272.1"
                     /translation="DAGANGPSRFIAQLEHGLYKGRPGMMTVATARVPVPVRGAAAVM
                     IPNDEETRARVRALLERGLSPSALGTWLRCPLDFWFRYVRGLKEPDVPGALIGADVMG
                     SALHAVMEDLHRPWLGRPLDADTVEAAIPGIREAILARFAKDFSLDHLSQGQPLLQTG
                     MAVQAVENYLRAEVRAVREGRRITPLALEAELRTQGPLRDLIPGLDPVFKGRLDRMDD
                     RDGVIHILDLKTGGADAKDLELKELNLDALRGKKGHAAQLLMYAWLHLLNNPDVAEVR
                     AGLQTLRSAGASDGIYLKLGTMDRITRTDLPAITALFADAVRALLDPEAGYAHDPDNT
                     YCVFCAKE"
     gene            complement(1470..>2032)
                     /locus_tag="PL106_09140"
     CDS             complement(1470..>2032)
                     /locus_tag="PL106_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33273.1"
                     /translation="PALAEGTPIRTNRSGQKHEEGVFNYMVYAGFGFDVMSASGQRYE
                     AGKEYTIVTIPVQGKGVVELVNDNWTSVPTNNGDYYVSLGGYDKTGVIYKGMAATTDL
                     DGSVNIKPNPNHGLFQFSFFCDEPSDLRVEVVNNLGQTSFSESLRGFSGSYVKDMDLT
                     RESEGPYYLKITRGGNTSTHKIVYQR"
BASE COUNT          362 a          610 c          659 g          401 t
ORIGIN      
        1 cgatgcgggc gcgaatggcc cgagccgttt catcgcgcag ttggaacacg ggctgtacaa
       61 ggggcgcccc ggcatgatga ccgttgcaac ggcccgtgtg ccggtaccgg tgcggggagc
      121 ggccgcggtg atgataccga atgacgagga gacacgggcc cgggtgcgcg ccctgctgga
      181 acgcggtctg tcgccttcag cgctgggcac ctggttgcga tgcccgctgg acttctggtt
      241 ccggtacgtg cgcgggttga aggagcccga cgttcccggt gcgctcatcg gtgccgacgt
      301 gatgggcagc gcgttgcacg cggtgatgga ggacctgcat cgcccctggc tcggtcggcc
      361 gctcgatgcg gatacggtgg aagcggcgat accgggtatc cgggaggcca tcctggcccg
      421 attcgccaag gacttctcct tggatcattt atcgcaaggc cagcccctgt tgcaaacggg
      481 catggccgtg caggccgtgg agaactactt gcgggccgag gtgcgagccg tacgcgaagg
      541 tcgacgcatc acgccgctcg cactggaagc ggaattgaga acgcagggcc cattgcgcga
      601 cctgatcccc ggtctggatc ccgtgttcaa gggacgtttg gatcgcatgg acgatcgcga
      661 tggcgtgatc cacatcctcg acctcaagac cggaggtgcc gatgccaagg accttgaact
      721 gaaggaactg aacctcgatg cgctgagagg aaagaagggc cacgccgctc aactgctcat
      781 gtacgcgtgg ctccacctgt tgaacaaccc cgatgtggcg gaagtgcgtg ccgggttgca
      841 gaccctgcgc agtgccgggg ccagcgacgg catctacttg aagctgggta ctatggaccg
      901 gatcacacgc acggatctcc cagccatcac cgccctcttc gccgatgcgg tgcgggcact
      961 gctcgatccg gaggccgggt atgctcatga cccggacaac acgtattgcg tgttttgtgc
     1021 gaaggagtga gtgtggctat cgagcgtggt ttttccagct gttgtaccgg acacgttcct
     1081 ggagccggcc atcgcttgga gctcggctga tgcgggagtg ggccgatgca tcctgcactt
     1141 cgacgggtcc agtgggagag ttcatgacgg catcgatagc ccccacttcg gcagcgcaga
     1201 tgatgcaggc agtggctcgc tgccccacat gagcgcatcc ccatgaaccc atgcaacgtc
     1261 gaactgatcc cgccggagcg catccacccc gggttgcaca ttggacgttc aagaaggagc
     1321 ttgaacatat accagccggg acgtcgccta ccaggggcac gctgtgcgaa gccgggtact
     1381 cgaggtgacc ggaacgctca tgcgggctct ccatgaatgg acaaaggggc cgtggagggc
     1441 cccttcatcc gatccggtcc gggtgtcgat cagcgctgat acacgatctt gtgcgtgctg
     1501 gtattgccac cgcgtgtgat cttcaggtag taggggcctt cgctctcgcg ggtgaggtcc
     1561 atgtccttca catagcttcc actgaatccg cgcagcgatt cgctgaagct ggtctggccc
     1621 aggttgttca ccacttccac gcgcaggtcg ctgggttcgt cgcagaagaa cgagaactgg
     1681 aacaggccat ggttcggatt cggtttgatg ttgacgctgc cgtccagatc ggtggtggcg
     1741 gccatgccct tgtagatgac gcctgtttta tcatacccac cgagtgacac atagtaatca
     1801 ccattgttgg tgggtacgct ggtccaattg tcgttcacca gttccaccac gcccttgcct
     1861 tgcaccggga tggtgacgat ggtgtactcc ttgcccgcct cgtaacgctg gccgctggcc
     1921 gacatcacgt cgaagccgaa ccccgcgtac accatgtagt tgaacacgcc ttcctcatgc
     1981 ttctgccccg agcggttcgt acggatgggc gtgccctcgg ccagtgccgg cg
//
LOCUS       DAOQOC010000545         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36767_length_2032_cov_3.383915, whole genome shotgun sequence.
ACCESSION   DAOQOC010000545 DAOQOC010000000
VERSION     DAOQOC010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36767_length_2032_cov_3.383915"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /gene="rodA"
                     /locus_tag="PL106_09145"
     CDS             <1..877
                     /gene="rodA"
                     /locus_tag="PL106_09145"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNA33274.1"
                     /translation="PVTIGKQTWSCKDVGTALTFGAFILVLYREGLSGNILLLAIGVA
                     ILAVLSLILRESMFHIPFWGREVSGQYFLIGIITLTCLFAFWFISRFVLKGQRKRLYG
                     FILFTLIGSAVFISAFDFVFENVLSERHRTRIGVTLGLVYDPQGLGYNAEQSKTAIGS
                     GGLIGKGYLKGTFTKYKYVPEQQTDFIFCTVGEEWGFAGTTLVVLLFGALILRCIQIG
                     ERQRSRFTRIYAYCVASIFFLHLMINVGMAIGLAPVIGIPLPLFSYGGSSLVGFTLLL
                     ATLVRLDAERLSQLR"
     gene            complement(929..1387)
                     /locus_tag="PL106_09150"
     CDS             complement(929..1387)
                     /locus_tag="PL106_09150"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNA33275.1"
                     /translation="MALKHRISLRIAIAAIPLLLSSLCSAQEAPYRAALKDYLEASNA
                     MGSFGTAIDQMIGMQREEHSDLPPEFWDSLGKEMRLSIDDLVDMLAPVYERHLSIEDL
                     QAVTTFYRSPSGSKFAEASPVVMQESMAVGAEWGSKLGERIMKRIQEEGR"
     gene            complement(1429..>2032)
                     /locus_tag="PL106_09155"
     CDS             complement(1429..>2032)
                     /locus_tag="PL106_09155"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA33276.1"
                     /translation="ILTAEGKETPVGDAGIVDVRSFVDGLAPFRTKDKLFGFMNSEQQ
                     VVIPAQFLSVGYFSGGLAWAKDKSEKLGFIDKQGAWVVKPAYDAAKEMDPASGLARVK
                     SGEAWFYVDRTGKEVRVADATGLGDFSDGLAEAKRGELTGFIGPDGTWVIEPKFNGAR
                     AFKNGYAAVKQGEQWGFIDTGGNWVVQPGFDAVKDMEKFQ"
BASE COUNT          346 a          659 c          588 g          439 t
ORIGIN      
        1 tccggtgacc atcggcaagc aaacatggtc atgcaaggac gtaggcaccg cgctcacgtt
       61 cggcgcgttc atcctggtgc tgtaccgcga gggactgtcg ggcaacatcc tgctgttggc
      121 catcggtgtg gccatcctgg ccgtgctctc actcatcctg cgcgagagca tgttccacat
      181 ccccttctgg ggccgggagg tctccggtca gtacttcctc atcggcatca tcacgctcac
      241 ctgcctgttc gcgttctggt tcatcagccg gttcgtgctg aagggtcaac gcaagcggtt
      301 gtacggcttc atcctcttca cgctcatcgg atcggccgtg ttcatcagcg ctttcgactt
      361 cgtgttcgag aacgtgttga gcgagcggca ccgcacacgt atcggcgtga ccctcggtct
      421 ggtgtacgat ccccaaggac tgggatacaa tgcggagcag agcaagacgg ccatcggcag
      481 cggtggtctt atcggcaagg gctacctgaa gggcacgttc accaagtaca aatacgtgcc
      541 cgagcagcag accgacttca tcttctgcac ggtaggggag gaatggggct tcgcgggtac
      601 caccctggtc gtgctgctgt tcggtgccct gatcctgcgg tgcatccaga tcggcgaacg
      661 gcagcgatca cgcttcacac ggatttacgc ctactgcgtg gccagtatat tcttcctgca
      721 cttgatgatc aacgtgggca tggccatcgg gttggcaccg gtgatcggga tcccgctgcc
      781 gctcttcagc tacggcggca gctcgctcgt gggcttcacg ctcctgctgg ccaccctggt
      841 ccgcctcgac gctgaacggc tcagccagtt gcgttgagca agctccccgg ccatcccgga
      901 ccggtcgtca tcgccaccaa agcgcaggtc atcgcccttc ctcctgtatc cgcttcatga
      961 tgcgctcgcc gagcttgctg ccccattccg cgccgaccgc catgctctct tgcatcacca
     1021 cgggcgatgc ttcggcgaat ttggagccgg acggactccg atagaatgtg gtgacggctt
     1081 gcaggtcctc gatggacagg tgccgctcgt acaccggtgc gagcatgtcc accagatcgt
     1141 cgatggacag ccgcatctcc ttgccgagac tgtcccagaa ttcggggggc aggtcgctgt
     1201 gctcttcccg ttgcatcccg atcatctgat cgatcgccgt gccgaacgag cccatggcgt
     1261 tgctggcttc caggtagtct ttcagggccg cgcggtacgg ggcctcctgt gcggaacaca
     1321 acgatgaaag gagcaggggg atcgcggcta tcgcgatgcg gagactgatg cggtgcttga
     1381 gggccatggt ctcgggttcc gggatcgcgc gaaagtacct gaaggttctc actggaactt
     1441 ctccatgtcc ttcaccgcgt cgaagcctgg ctgcaccacc caattccccc cggtgtcgat
     1501 gaaaccccat tgctcgcctt gttttaccgc ggcgtaaccg ttcttgaatg cacgcgcacc
     1561 gttgaacttc ggttcgatca cccatgttcc gtccgggccg atgaagccgg tcaactcccc
     1621 acgcttggcc tccgcgagcc catcgctgaa atcgccgaga cccgtggcat cggcaacgcg
     1681 tacctcttta ccggtacggt ccacgtagaa ccaggcctcg ccgctcttca cccgggccag
     1741 gccgctcgca gggtccattt ccttggcggc atcatatgcg ggtttgacca cccacgcacc
     1801 ctgcttgtcg ataaagccga gtttctcact cttgtccttg gcccaggcca gtccgccgct
     1861 gaaatagcct accgacagga attgcgccgg gataacgact tgttgctcgc tgttcatgaa
     1921 gccgaagagc ttgtccttgg tacggaatgg ggcgaggccg tccacgaaac tcctcacatc
     1981 cacgatgccc gcatcgccca cgggtgtctc tttgccctcg gccgtgagga tg
//
LOCUS       DAOQOC010000546         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36782_length_2031_cov_4.261134, whole genome shotgun sequence.
ACCESSION   DAOQOC010000546 DAOQOC010000000
VERSION     DAOQOC010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36782_length_2031_cov_4.261134"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PL106_09160"
     CDS             complement(<1..891)
                     /locus_tag="PL106_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA33277.1"
                     /translation="MSKKILVIGSSGQIGTELVEGLRTRFGAESVVASDIKESQVKQD
                     GPFVLVNAMDRHGIERIIDKHAITEVYLLAALLSATAEKDPAFAWKLNMESLLIILEL
                     AREGRLKKVYWPSSIAAFGPTTPKDGTPQHTITEPSTVYGISKLAGEGWCNYYHHRYG
                     VDVRSIRYPGLIGWKSAPGGGTTDYAVHIFHEAIKHGAYTCFLGPKSMLPMMYMPDAI
                     RATIELMEAPAERIKERTSYNLAGLSFTPEQIAAEVAKHIPGFQIAYAPDVRQNYADS
                     WPNSIDDSAARHDWGWKPEYD"
     gene            946..>2031
                     /locus_tag="PL106_09165"
     CDS             946..>2031
                     /locus_tag="PL106_09165"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) hydroxylase"
                     /protein_id="HNA33278.1"
                     /translation="MDPHQLRNLYGPDVLRARLEREGVERTTLSFYRYVRLAEVEVLR
                     HVLYQEWEKLGVLGRIYISQEGINAQVSLPTADLAAFRANLDDREAFRDVPWKIAVED
                     DGKSFLKLTIKVRQKIVADGLADDAFDVTNVGAHLDAKTFNRKMEEGALVIDMRNNYE
                     CLIGHFEGAYLPKADTFRGAIEEVVEMLGERVRGSEGEKKRKSEEVKAGEEVEANGEW
                     SMVNGERAEANSQPATRQHATPPALDQPEILLYCTGGIRCEKASAYLKHEGFTNVSQL
                     HGGIIDYARQLKAEGLKSKYLGQNFVFDERLAERITDDVVSTCMQCGAPCDRIGNCHE
                     ATCNMLLVQCEACATKYSDCCSPSCREI"
BASE COUNT          391 a          571 c          673 g          396 t
ORIGIN      
        1 atcgtattcc ggcttccatc cccaatcgtg ccgggcggcg ctgtcgtcaa tgctgttggg
       61 ccaggaatcg gcgtagttct ggcgtacatc gggggcgtat gcgatctgga agcccgggat
      121 gtgcttggcc acttcggcgg cgatctgctc cggggtgaaa ctgagccctg cgaggttgta
      181 gctggtgcgc tccttgatgc gctcggccgg ggcttccatc agttcgatgg tggcgcggat
      241 ggcatcgggc atgtacatca tgggcagcat gctcttgggt cccaggaagc aggtgtacgc
      301 accgtgcttg atcgcttcgt ggaaaatatg taccgcatag tcggttgtgc cgccgccggg
      361 tgcgctcttc cagccgatga ggcccgggta gcggatgctc cgtacatcaa cgccgtagcg
      421 gtggtggtag tagttgcacc agccttcgcc ggccagcttg ctgatgccat agaccgtgct
      481 gggctcggtg atggtgtgct ggggcgttcc gtccttgggc gtggtgggcc cgaatgcggc
      541 tatactgctt ggccagtaca cctttttcaa ccgtccttcc cgcgccagtt ccaggatgat
      601 gagcaggctc tccatattga gcttccacgc gaaggccggg tccttctcgg cggtggcgct
      661 gagcagcgcg gccagcaggt acacctcggt gatcgcgtgc ttgtcgatga tgcgttcgat
      721 gccgtggcgg tccatggcgt tcaccaggac gaagggccca tcctgtttca cctgggattc
      781 cttgatatcg gaggccacga cgctctccgc accgaagcgc gttcggaggc cttccacgag
      841 ttcggtgccg atctggccac tggagccgat gacgaggatc ttcttggaca tgtggccaaa
      901 tctattggtg accgggctgc gatccgtgct ccgtaccttc gccacatgga tccgcaccag
      961 ttgcgcaacc tgtatgggcc ggatgtactt cgggcaaggc tggagcgtga aggtgtggag
     1021 cggaccaccc tttccttcta ccgctatgtg cggctggccg aggtggaagt actccggcac
     1081 gtgttgtacc aggaatggga aaagctcggc gtgctgggcc gcatctacat ctcccaggaa
     1141 ggcatcaacg cgcaggtgag cctacccacg gccgatctcg cggcgttccg cgccaacctc
     1201 gatgatcgtg aagccttccg ggatgtgcca tggaagatcg ccgtggagga cgatggcaag
     1261 agcttcctga agctcacgat caaagtgcgg caaaagatcg tggccgatgg cctggccgat
     1321 gatgccttcg atgtgaccaa cgtgggagcg cacctcgacg cgaagacctt caaccgcaag
     1381 atggaagaag gcgccttggt gatcgacatg cgcaacaact acgagtgcct catcgggcat
     1441 ttcgaggggg cctacctgcc caaggctgac acgttccgcg gggcgatcga ggaggtggtt
     1501 gaaatgctgg gtgagcgggt gagagggagc gagggtgaga agaagagaaa aagtgaggaa
     1561 gtgaaggcgg gggaggaagt agaggcgaat ggcgaatggt caatggtgaa tggggaacgc
     1621 gcagaggcca actcgcaacc cgcaacacgc caacacgcaa cacctccagc tctcgaccaa
     1681 cccgagatcc tcctctactg caccggcggc atccgctgcg agaaggccag tgcctatctg
     1741 aagcatgagg gcttcaccaa cgtgagccag ttgcacggcg gcatcatcga ctacgcgcgg
     1801 cagctgaagg cggaagggct gaagagcaag tacctcggcc agaacttcgt gtttgatgaa
     1861 cgcttggccg agcgcatcac cgacgatgtg gtgagcacct gtatgcagtg tggcgcaccg
     1921 tgcgatcgca tcggcaactg ccatgaggcc acgtgcaaca tgctgctggt acaatgcgag
     1981 gcctgcgcaa cgaagtacag cgactgctgc tcgccgagct gccgcgagat c
//
LOCUS       DAOQOC010000547         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36795_length_2031_cov_3.268219, whole genome shotgun sequence.
ACCESSION   DAOQOC010000547 DAOQOC010000000
VERSION     DAOQOC010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36795_length_2031_cov_3.268219"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PL106_09170"
     CDS             complement(<1..1693)
                     /locus_tag="PL106_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNA33279.1"
                     /translation="MIRSARPLLLTLMLLFSILTVAQDARTVVRQWLEQHHSSLGLTE
                     KDATDWTVTSSSTDKKSVTYLYIRQVVQGLPVHGAEASFAVRAGAVVSFGNRLIADAQ
                     TRATSPSPGIHATDAIKAAAAQLGLPVAEPGLRYGSSATDLLFDGSGISRDPIPAQLI
                     YQALPDGSIMLAWDLTIRTTDGRHWWHVAVDAHTGDILRTNDYTTHCAVPFPTGRGYR
                     ALDDLALPSSPLAPPPDGSSYRVFPFPTESPSHGPHVLVTDPADPEASPFGWHDLNGV
                     EGSETSTTRGNNVFAYEDMDNNDAPGASPEGGGSLTFDFPYQPPQEPQDYLDAAITNL
                     FYSCNVLHDVWYHYGFDEESGNFQDLNATGLGLDGDGVIAEAQDGGGMNNANFSTPPD
                     GGQGRMQMYIWRAENDSLLVINTPQSIAGAYFCSLAGFGPALPETPLTAGIVLVEDDQ
                     DPNNDGCETITNGADIEGNIALVDRGQCTFVTKVLALQQEGALAVIVVNNVPGEPVAM
                     GGDDDGINIPAVMISQADGATIKEALLNGAVSGTLQGTGMEDLFEMFGMNGQQAQW"
BASE COUNT          359 a          623 c          655 g          394 t
ORIGIN      
        1 cccactgcgc ctgctgcccg ttcatgccga acatttcgaa caggtcctcc atgccggtgc
       61 cctgcagggt gcctgaaacg gcgccattga gcagcgcttc cttgatcgtc gctccatccg
      121 cctgcgagat catcacggcg ggaatgttga tcccatcatc gtcgccgccc atggctaccg
      181 gctcgccggg cacgttgttc accacgatca ccgcgagggc accctcctgt tgtagcgcga
      241 gtaccttggt cacgaaggtg cactgtccac ggtccaccaa tgcgatgttc ccctcgatgt
      301 ccgcaccatt ggtgatcgtt tcacagccgt cgttgttggg gtcctggtcg tcctccacca
      361 gaacgatacc ggcagtaagt ggtgtttctg gcagggccgg accgaaaccg gcgagggagc
      421 agaaataggc gcccgcgatc gactggggag tgttgatcac cagcaggctg tcgttctccg
      481 cccgccagat gtacatctgc atccggccct ggccaccatc gggcggcgtg ctgaagttgg
      541 cgttgttcat gcccccgccg tcctgggcct cggcgatcac cccgtcgccg tccaggccga
      601 gcccggtggc attgagatcc tggaagttgc cgctctcctc atcgaaacca taatgatacc
      661 atacatcgtg cagcacgttg cagctgtaga acaggttggt gatggcggca tcgaggtagt
      721 cctgcggttc ctggggcggc tggtagggga aatcgaaggt caatgagccg cctccttcgg
      781 gactcgcgcc cggggcatcg ttgttgtcca tgtcctcata cgcgaacacg ttgttgcccc
      841 gcgtggtcga ggtttcggaa ccctcgacac cgttcaggtc gtgccatccg aatggcgatg
      901 cctcggggtc ggcgggatcg gtcaccagta cgtgcgggcc gtgcgagggg ctctccgtgg
      961 ggaacgggaa gacgcggtaa ctggagccgt ccggcggtgg cgcgagcggt gaagaaggca
     1021 gggccaggtc gtccagggcc cggtagcctc gtcctgtcgg gaatggtacc gcacaatggg
     1081 tggtgtagtc gttcgtgcgc aggatgtccc ccgtgtgcgc atccacggcc acatgccacc
     1141 agtgccgccc atcggtggtg cggatcgtca ggtcccaggc cagcatgatg ctgccgtccg
     1201 gcagggcctg gtagatgagt tgcgctggaa tggggtcgcg ggagatccca ctcccatcga
     1261 acaagaggtc ggtcgccgaa gatccatacc gcaacccggg ttccgcgacc ggcaggccga
     1321 gctgcgccgc tgccgccttg atcgcatcgg tggcatggat accgggcgaa ggggatgtgg
     1381 cccgggtttg cgcatcggcg atcagccggt tgccaaaaga taccacggcc cctgcacgca
     1441 ccgcgaagct cgcctcggcc ccatgcacgg gcaacccttg caccacctgc cggatgtaga
     1501 ggtaggtgac actcttcttg tccgtactgc tgctggtgac ggtccagtcc gtggcgtcct
     1561 tctcggtaag tcccaaggac gaatgatgct gctccaacca ttgccgaacc acggttcggg
     1621 cgtcctgcgc cacggtgagg atgctgaaga gaagcatgag tgtcaggaga aggggtcggg
     1681 cagaacggat catggccggg aggtagggtg ggactcgtac gatgggaaag ataggcgata
     1741 gcctcatgag gcacggtcgg aaccggcctg ctctgtcggg tcgggccctg cttgcgactt
     1801 ttgcccacgg atggagcatt gatcgcgccc tcgaaggtcg caccgctttg atcgcacgga
     1861 caggaccagg cgatcagatc ctcttcttcg cgcttcatgg actgcggaag gcaaccgatc
     1921 atggcttggc aacgtccccg tgttgaccat gaaagcacat gctattcccc tagtggcggc
     1981 attggtgata gccacagatg ccacggcaca agttcccatc atcgactaca g
//
LOCUS       DAOQOC010000548         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36841_length_2030_cov_2.856203, whole genome shotgun sequence.
ACCESSION   DAOQOC010000548 DAOQOC010000000
VERSION     DAOQOC010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36841_length_2030_cov_2.856203"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PL106_09175"
     CDS             <1..257
                     /locus_tag="PL106_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA33280.1"
                     /translation="IKRPFLVGSLWQGLVASVLAIGLLAGLIHVLMRYMPDLRDPTDA
                     LMLAVIFGGVIVLGLVISLLSTWFAVRRYLRMNTDELNWS"
     gene            363..611
                     /locus_tag="PL106_09180"
     CDS             363..611
                     /locus_tag="PL106_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNA33281.1"
                     /translation="MADAREDELAFTRHNYRLLLIGIGIVILGYILMAGGGSGDPEVF
                     NAQEIFSPRRITVAPIVCLIGYLFVVYAIMKKPSTKVG"
     gene            708..1502
                     /locus_tag="PL106_09185"
     CDS             708..1502
                     /locus_tag="PL106_09185"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNA33282.1"
                     /translation="MNVLHAIILAIIEGLTEYLPVSSTGHMIIGSSLMGIAHEEFTRI
                     FTVGIQFGAILSVVVLYWRRFFEGFSTYPLLVAGFIPAAIAGLLFKDAIDLLLENIGV
                     VAAALFIGGIVLLFIDRWFPHDHEDRQEPLSWKDALFIGCFQVIAMVPGISRSAATII
                     GGLTRKLSRAQAAEFSFLLAVPTMCAATAKTLWDHYQEGGMFTSEQWKLFGIGNVVAF
                     VVAALAIKGFVGFLNKHGFKPFGWYRIGLGALLLALLWAGVDLRVI"
     gene            1505..>2030
                     /locus_tag="PL106_09190"
     CDS             1505..>2030
                     /locus_tag="PL106_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HNA33283.1"
                     /translation="MGPIDPEAGAVLLVDKPATWTSFNVVAKLRGALHKLCGHRVKVG
                     HAGTLDPLATGLLILGTGRMTKRLPELTAQDKEYIATLRLGQTTASYDAETPVLEERP
                     WEHITTADLQRVLTTFLGDTLQRPPDFSAKRFQGERGYNLVRWGEPVVMTPVPVRIDA
                     IELLALNGPEATLRV"
BASE COUNT          353 a          686 c          609 g          382 t
ORIGIN      
        1 tcatcaagcg gcccttcctg gtgggaagcc tctggcaggg cctggtggcc tcggtgctgg
       61 ccatcggcct gctggccggg ctcatccacg tgctcatgcg ctacatgccc gacctgcgcg
      121 accccaccga tgccctcatg ctcgccgtga tcttcggcgg ggtgatagtt ctcggccttg
      181 tcatttcttt gctgtccacc tggttcgccg tgcgccgcta cctgcgcatg aacaccgacg
      241 aactgaactg gagttgaacc gctgaatatt tcaccacaga ggcacagagg gcacagaggg
      301 aaaggctcaa cacgtattct ccgtgtcctc tttgcctctg tggtgaaccc aagaccagac
      361 ccatggccga cgcacgcgag gacgaactgg cgttcacccg ccacaactac cggttgctgc
      421 tcatcggcat cggcatagtg atcctgggct acatcctgat ggccggtggg ggcagcggtg
      481 accccgaggt cttcaacgcc caggagatct tcagtccgcg ccggatcacc gtggcgccga
      541 tcgtctgcct catcggctac ctcttcgtgg tgtacgccat catgaagaag ccgtcgacca
      601 aggtcggttg agccagcgca ccgcatcgtt ggtggcccgg tgcgcgggcc gggatcgcca
      661 cggaccacgc gcaacacgta tcccccgctg aaaatcgcgc aacgcccttg aacgttctcc
      721 acgccatcat cctggccatc atcgagggcc tcacggaata cctgccggtg agcagcaccg
      781 gccacatgat catcggcagc agcctcatgg gcatcgcaca cgaggagttc acgcgcatct
      841 tcaccgtggg catccagttc ggcgcgatcc tcagcgtggt ggtactttat tggcgccgct
      901 tcttcgaggg cttcagcacc tacccgctgc tagtggcggg cttcattccg gcggccatcg
      961 ccgggctgct cttcaaggat gctatcgacc tcctgctgga gaacatcggc gtggtggcgg
     1021 cggcgctgtt catcggcggc atcgtgctcc tgttcatcga ccggtggttc ccgcacgacc
     1081 acgaggatcg ccaggaaccg ctcagttgga aggatgcgct cttcatcggc tgcttccagg
     1141 tgatcgccat ggtgcccggg atcagccgca gcgccgccac catcatcggc gggctcacgc
     1201 gcaagctctc cagggcgcag gcggcggagt tctccttcct gctggccgta cccaccatgt
     1261 gtgccgccac ggccaagacg ctctgggacc attaccagga aggcggtatg ttcacttcgg
     1321 aacagtggaa actcttcggc atcggcaacg tggtggcctt cgtggtggcc gcactcgcca
     1381 tcaaaggctt cgtcggcttc ctcaacaagc acggattcaa acccttcggc tggtaccgca
     1441 tcggcctggg agcgctgctg ctcgccttgt tgtgggccgg ggtggatctg cgtgtgatct
     1501 gaccatgggg cccatcgatc ctgaagcggg cgccgtgctg ctggtggaca agcccgccac
     1561 gtggaccagc ttcaacgtgg tggccaagtt gcgtggcgcg ctgcacaaac tctgtggcca
     1621 ccgggtgaaa gtgggccacg cgggcaccct cgatccgctg gccaccggcc tgctcatcct
     1681 gggcacgggc cggatgacca aacgacttcc cgaactcacc gcgcaggaca aggaatacat
     1741 cgccacgctg cgcctgggcc agaccacggc gagctacgat gccgaaacac cggtattgga
     1801 ggaacggccc tgggagcaca tcaccacagc ggacctccaa cgcgtcctca ccacctttct
     1861 gggcgacacc ctgcaacgcc cccccgactt ctcggccaaa cgcttccagg gcgaacgcgg
     1921 ctacaatctg gtgcgctggg gagagccggt ggtcatgacc ccggtacccg tgcgtattga
     1981 cgccatcgaa ctgctcgctc tcaacggtcc cgaggccacc ttgcgcgtag
//
LOCUS       DAOQOC010000549         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36868_length_2029_cov_3.896657, whole genome shotgun sequence.
ACCESSION   DAOQOC010000549 DAOQOC010000000
VERSION     DAOQOC010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36868_length_2029_cov_3.896657"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PL106_09195"
     CDS             complement(<1..1262)
                     /locus_tag="PL106_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33284.1"
                     /translation="MRRILAIGALIVLGSVVGTARAQEYLGYPDYAAWKAATYGVAID
                     PHPQPAPDAGTPGDTRGGGTGTCECWIEPDGSYTTINNNSEWNASGWSSTDDGSYGPI
                     NLPFQFYLYGQFWNTAYININGNVSFGQYFGTFSSTGFPVSGYTMVAPFWADVDLGGT
                     CAGCNQVSFKVTPTALYVNWKNVGYYPSQTNLKNTFQLIITDGTDPIVPDGANVSFCY
                     QDMQWTTGGASGGTGGFGGTPASVGANQGNGVDYIQFGRFDHAGTDYDGPLGANDGVS
                     FLDDQSFRFSTDVTQANVPPVVTGQSACDTIVLCVNETSTLEVSFLSPEPGQITVPTV
                     SAPTFTGPVNTVNATNGLNAQITVEVTPAMADVGYQTITFTGTDDGTPVGTTVLNVVI
                     QVLSAASMEPGSADVCSDGTAVDMYALIG"
     gene            complement(1267..>2029)
                     /locus_tag="PL106_09200"
     CDS             complement(1267..>2029)
                     /locus_tag="PL106_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33285.1"
                     /translation="GTTPATVEKTHQAAPAAKPDPTKEQQVAPVAVTVAPRTIEDHGS
                     PTPVVHVSDKDERTPAAQAAPTEHKESNRAVETHAEDRSKVLPPPTPEGQATVNAVLQ
                     DLGAEQRVEPAADKQESISPPAKQTPDVSGLPDRSTDTEGGTATEEAGSTAALENIPE
                     MTVLIPTAFSPNGDGTNDELFVSVQNYQKAAVRIFSASNNALVFAADNLEVKWNGRVM
                     NGSQPCEPGMYFYALEITDANGRTSSKGEVVRLFR"
BASE COUNT          333 a          632 c          636 g          428 t
ORIGIN      
        1 ccgatcaacg cgtacatgtc caccgccgtg ccatcgctgc acacgtccgc tgatccgggt
       61 tccatggaag ccgcggagag cacttggatc accacgttga ggacggtggt ccctacgggc
      121 gtgccgtcat cggtacccgt gaaggtgatg gtctgatagc ccacgtcggc catcgccggc
      181 gtcacctcca cggtgatctg agcattgagc ccgttggtgg cgttcacggt gttcaccggg
      241 ccggtgaagg tgggcgcgct caccgtgggc acggtgatct gtccgggctc cggggacagg
      301 aagctgacct ccagggtgga ggtctcgttc acgcacagca cgatggtgtc gcaagctgat
      361 tgcccggtga ccacaggtgg cacgttggcc tgtgtaacat cggtggaaaa acggaaggac
      421 tgatcgtcca ggaagctcac accgtcattg gccccgagcg gcccgtcgta gtcggtgccc
      481 gcatggtcga aacgtccgaa ctggatatag tccaccccgt tgccctgatt ggcgcccaca
      541 ctggcgggtg tgccgccgaa cccgcccgta ccaccactgg ccccacccgt ggtccactgc
      601 atatcctggt aacagaagct cacgttggcc ccgtcgggaa cgatcgggtc agtgccatcg
      661 gtaatgatca gctggaacgt gttcttcagg ttcgtctgcg aggggtagta gcccacgttc
      721 ttccaattca cgtacagggc ggtgggcgtc actttgaagc tcacctggtt gcaccccgcg
      781 caggtgcccc ccaggtccac atcggcccag aacggcgcca ccatggtgta accggaaacg
      841 gggaaacccg tggatgaaaa tgtaccgaaa tactgaccga aggagacgtt gccgttgatg
      901 ttgatgtacg cggtgttcca gaactgcccg tacaggtaga attggaaggg caggttgatg
      961 gggccgtaac tgccgtcatc ggtgctgctc catccgctgg cgttccactc ggagttgttg
     1021 ttgatggtgg tatacgaccc atcgggctcg atccagcact cgcaggtgcc ggtaccgcca
     1081 ccgcgtgtat cgcccggtgt tcccgcatcc ggtgcgggct gcgggtgcgg atcgatggcc
     1141 actccgtatg tggcggcctt ccatgccgca taatccgggt agccgaggta ctcctgggcc
     1201 ctggccgttc caacgaccga gcccagcacg atcagtgccc ctatggccag gatgcgcctc
     1261 atgggatcaa cggaacaagc gtaccacttc ccccttgctc gacgtacggc cgttcgcatc
     1321 ggtgatctcc agcgcataga agtacatacc cggctcgcac ggctgactac cgttcatgac
     1381 ccggccgttc catttgacct caaggttatc ggccgcgaag accagggcgt tgttgctggc
     1441 cgaaaagatg cgcacagcgg ccttttggta gttctgtacc gagacgaaaa gctcgtcgtt
     1501 cgtaccatcg ccgttcgggc tgaaagccgt ggggatcagc accgtcattt ccgggatgtt
     1561 ctccagtgcc gccgttgaac cggcctcttc agtagctgtt cctccttcgg tgtccgtgct
     1621 gcggtcaggc aacccactga cgtcaggcgt ttgtttggcc ggtggtgata tcgactcctg
     1681 cttgtccgcc gccggttcga cccgctgctc cgcacccaga tcctgtaata ccgcgttcac
     1741 cgtggcctgg ccctcggggg tgggtggcgg cagcaccttg gatcggtcct ccgcgtgtgt
     1801 ttcaacggcc ctgttcgact ccttgtgctc agtgggggcg gcctgtgctg ccggggtccg
     1861 ctcatcctta tcggacacat gcaccacagg ggtcgggctg ccgtggtcct cgatcgttcg
     1921 tggtgctacg gtaacggcca ccggagcgac ctgttgttcc ttggtgggat cgggcttcgc
     1981 cgctggcgct gcctggtgcg tcttctccac agtggccggg gtcgtgcct
//
LOCUS       DAOQOC010000550         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36919_length_2028_cov_3.686265, whole genome shotgun sequence.
ACCESSION   DAOQOC010000550 DAOQOC010000000
VERSION     DAOQOC010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36919_length_2028_cov_3.686265"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>2028
                     /locus_tag="PL106_09205"
     CDS             8..>2028
                     /locus_tag="PL106_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNA33286.1"
                     /translation="MPNPLGDVNSLLQGRLGVVTRNELSSGYSVRGGNFDENLVYVND
                     IEVYRPFLVRAGQQEGLSFPNPDMIEKIHFSAGGFEARYGDKLSSVLDITYKRPREFS
                     GSASASFMGGSLSIANPMAKRRLRQVTGFRYRTLSAVLKGLDTQGDYTPVYTDLQSYW
                     TYDVSEKVEVGFLGVYSRNKYDVIPRDRETELGNFNQALRFNVYFEGQERTSYETFFG
                     ALNVNVKPRKDMFLKFTTSAFRTYETERFDILGQYRLSELDRDLGSDNFGQPIKVLGV
                     GTYLDHARNELRATVLTATHRGTWHSLRTATRHHEWQWGVEARAERIQDRLNEWYLLD
                     SADYSIPYSVDPEHLRLRSSVKSRVDLRSIRSSAYVQHAWDWKGTNGSDWSLVTGVRA
                     QNWTYNGQTIVSPRLRVAWHPGWKKISAEGDTVQKDFSFWFATGLYQQAPFYRELRDM
                     HGVLNPELRAQRSIHFILGGDRQFKMWDRPFKWSTELYYKILDDLIPYELDNVRIRYY
                     AKNDAKGYAMGIDTKLNGEFIPGIESWMALSVMKTEEDILDDQYYERYNSDGKVIIPG
                     YTLNNVAVDSALKQPGYIPRPADQRVSFALFFQDEMPGAPTFKVHVSLNFGTGLPYGP
                     PNNSRYEDVLRGPLYRRVDIGFSKQLLGAKGQEKKGFLRHIDDAWVSLEV"
BASE COUNT          443 a          607 c          629 g          349 t
ORIGIN      
        1 ccgcttcatg cccaacccat tgggcgatgt gaacagcctg ctgcaggggc gcctcggtgt
       61 ggtcacccgg aacgaactga gcagtggcta cagcgtgcgc ggcggcaact tcgacgagaa
      121 cctggtgtac gtgaacgaca ttgaggtgta ccggccgttc ctggtgcgcg cgggccaaca
      181 agaagggctc agcttcccga acccggacat gatcgagaag atccacttca gcgcgggtgg
      241 cttcgaggca cgttatggcg acaagctcag cagtgtgctg gacatcacct acaaacggcc
      301 gcgtgaattc tccggctcgg cctcggccag tttcatgggc ggatcgctca gcattgcgaa
      361 ccccatggcc aagcgccgcc tgcgccaggt caccggcttc cggtaccgaa cgctgagcgc
      421 cgtgctgaaa ggactggaca cccaagggga ctacaccccg gtgtacaccg acctgcagag
      481 ctattggacc tatgacgtga gcgagaaagt ggaggtggga ttcctggggg tgtacagccg
      541 gaacaagtac gatgtgatac cacgcgatcg ggagaccgag ctgggcaact tcaaccaggc
      601 gctgcgcttc aacgtgtact tcgaaggtca ggagcggacg tcctacgaga ccttcttcgg
      661 tgcgctcaac gtgaacgtga agccccgcaa ggacatgttc ctgaaattca ccaccagcgc
      721 tttccgcacg tacgagaccg agcgtttcga catccttggg cagtaccgcc tgagcgagct
      781 ggaccgcgat ctgggcagcg acaatttcgg gcaaccgatc aaagtgctgg gtgtgggcac
      841 ctacctggac catgcgcgca acgaattgcg ggccaccgtg ctcaccgcca cacaccgggg
      901 cacctggcac agcttgcgca ccgccacacg gcaccacgaa tggcaatggg gcgtggaagc
      961 ccgcgccgaa cggatccagg accggctgaa cgaatggtac ctgctggaca gcgcggatta
     1021 ctccatcccc tactccgtcg atcccgagca tttgcgcttg cgcagcagcg taaagagccg
     1081 ggtggacctg cggagcatcc gctccagtgc gtacgtgcaa catgcctggg actggaaggg
     1141 gaccaacggc agcgactggt cgctcgtgac cggcgtgcga gcgcagaact ggacgtacaa
     1201 cggccagacc atcgtgagtc cgcgattgcg tgtggcgtgg catcccggtt ggaaaaagat
     1261 cagtgctgaa ggggacaccg tgcagaagga tttcagcttc tggttcgcca caggcctgta
     1321 ccagcaagct cccttctacc gggagttgcg cgacatgcac ggcgtgctca accccgaact
     1381 ccgcgcccag cgcagcatcc acttcattct gggcggcgac cggcaattca agatgtggga
     1441 ccgccccttc aaatggagca ccgaactgta ttacaagatc ctggacgacc tgatccccta
     1501 cgaactggac aacgtgcgca tccgctacta cgcgaagaat gatgccaagg gctatgcgat
     1561 ggggatcgat accaagctga acggcgagtt catcccgggc atcgaatcgt ggatggccct
     1621 ctcggtgatg aagacggagg aggacatact ggacgaccag tactatgagc gatacaacag
     1681 cgatgggaag gtgatcatac cgggctacac gctcaataat gtggccgtcg acagcgcact
     1741 gaaacaaccc gggtacatac cgcgtcctgc ggatcaacgg gtgagtttcg cgctcttctt
     1801 ccaggacgag atgcccggtg cgcccacctt caaggtgcat gtgagcctga acttcggcac
     1861 gggactcccc tacgggcctc cgaacaactc gcggtacgag gacgtcttgc ggggcccgct
     1921 gtaccggcgg gtggacatcg gcttcagcaa gcaactactg ggtgccaaag gccaggagaa
     1981 gaaaggcttc ctgcggcaca ttgatgatgc gtgggtctcg ttggaggt
//
LOCUS       DAOQOC010000551         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_36981_length_2027_cov_2.755071, whole genome shotgun sequence.
ACCESSION   DAOQOC010000551 DAOQOC010000000
VERSION     DAOQOC010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36981_length_2027_cov_2.755071"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..382)
                     /locus_tag="PL106_09210"
     CDS             complement(182..382)
                     /locus_tag="PL106_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33287.1"
                     /translation="MKAARMETPEENASQEAQEPTSSYSGYSNEGLGFCPIKDLNLPT
                     SYDEVVTRFRKLQGERAVEVFN"
     gene            577..1224
                     /locus_tag="PL106_09215"
     CDS             577..1224
                     /locus_tag="PL106_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33288.1"
                     /translation="MIRPILLTVACAAASWCSAQVNEPGTVQVGVGGELGFFATHFVS
                     EMTVPFLGHKTSSHDDGAVTTSLPIDLQVGVARRWSIGISLDPGRYVDSAGTQPNAFL
                     VAGLQPRFYAVNGDHFALHLNAEVGYGVLKIRSNENTNGQFEDTYAGMHVRVGTEAQV
                     YFGRVFGLHAGLKYALHGMEWKRRDPENDLLDLLDYKARLRTSGLLVQLGLQVRF"
     gene            complement(1233..>2027)
                     /locus_tag="PL106_09220"
     CDS             complement(1233..>2027)
                     /locus_tag="PL106_09220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA33289.1"
                     /translation="LLRGVFPDARHFSEDALRWQYRDNPDGEVVGFNARLEGVLAAHY
                     VAIPLVATVDGREERGLLSLNTATHPSHQGKGLFTKLANATYAHAAEQGFGFVIGVAN
                     ANSTHGFVKKLGFQLVSPLRAMVGIGPLPYRTTGAKVRYERVWSPAALRWRLAHPCYP
                     YMLKHAGNTRLVLSERTQFGPRYVLGAARDGRFDGLAPTTNEGVRMKAWIGLDPDLDW
                     AGSLYVNVPMRLRPSPLNLILKDLTGRGRTLDADGIRFSALDFDIL"
BASE COUNT          377 a          652 c          630 g          368 t
ORIGIN      
        1 gaggatgccg cgctcctcct cacttccatt cttcttcacc aaccagcatc cgcgaatgaa
       61 gtaaccggat cttcgttatc gcctcagtaa caatgaagcc catgagccag gccaggtcgg
      121 acgggccacc aggaaatgcg aaagccccgg aagaccgggg ctcggatcgc actgatcagg
      181 ctcagttgaa cacctccacc gcccgctcgc cctgcagctt gcggaatcgg gtcaccacct
      241 cgtcgtaact ggtcggcagg ttcagatcct tgatggggca gaaacccaga ccctcgttgc
      301 tgtatccgct gtacgacgat gtcggctcct gcgcttcctg cgatgcgttc tcctccggcg
      361 tctccatccg tgctgctttc attgcttgtt gcgagagagg ggccgaatat agagcaagag
      421 gcaacccagc gccagccccc tcgtaccatg cgcgtacaga tcggtggcga tactcgggtt
      481 cctaccatgc cggatgccgg ttccgatcgc ccatgatcat gcggagaaca tgatcgaccc
      541 ggtgcaccgg atacattcgc cggaacaacc gtgaccatga tccgccccat tctgctcaca
      601 gtcgcctgcg ctgcggcttc ctggtgttcc gcccaagtga acgagcccgg cacggtgcag
      661 gtgggggtgg gcggggagtt gggcttcttc gccacgcatt tcgtgagcga gatgaccgtg
      721 cctttcctgg gccacaagac ctcctcgcac gacgatggtg cggtcaccac ctccctgccc
      781 atcgatctgc aggtgggcgt ggcccggcgc tggtccatcg gcatcagtct ggatcccggc
      841 cgttacgtgg attccgcggg tacgcagccc aatgccttcc tggtggccgg actacagccc
      901 aggttctatg cggtgaacgg ggatcacttc gccctgcacc tgaacgccga agtgggctac
      961 ggggtgctga agatccgatc caacgagaac accaacgggc agttcgagga cacgtatgcg
     1021 ggcatgcacg tgcgcgtggg cacggaggca caagtctact tcggcagggt gttcggcctg
     1081 catgcggggt tgaaatacgc attgcatggt atggaatgga agcgcaggga cccggagaac
     1141 gacctgctgg acctcctgga ctacaaagcc cggctgcgga ccagcggcct gttggtccaa
     1201 ttgggcctgc aagtgcgatt ctgatacctg tttcagagga tatcgaagtc cagcgcgctg
     1261 aaccggatgc cgtcggcgtc cagcgtacgg ccccgaccgg tgaggtcctt gaggatcagg
     1321 ttgagcgggg agggccgaag gcgcatcggt acattcacat acagggagcc ggcccagtcc
     1381 aggtcgggat ccaggccgat ccaagccttc atgcgcacgc cctcgttcgt ggtgggggcc
     1441 agaccatcga atctcccgtc ccgcgcggca cccagcacgt accgcggacc gaactgcgtg
     1501 cgctcgctga gcaccagccg cgtgttgccc gcatgtttga gcatgtacgg gtaacaggga
     1561 tgcgccagcc gccaccgtag cgcggccggg ctccagacac gctcatatcg caccttcgca
     1621 ccggtcgtgc ggtagggcag ggggcctatg cccaccatgg cccgcaaggg cgaaacgagc
     1681 tggaacccca gcttcttgac gaagccgtgc gtgctgttcg cgttggccac accgatcacg
     1741 aacccgaaac cctgttcggc cgcgtgcgca tacgtggcgt tggccagttt ggtgaagagc
     1801 cctttgccct ggtgcgaagg atgcgtggcg gtgttcaggg aaagcagccc gcgttcctcg
     1861 cggccatcca cggtcgcgac cagtggaatg gcaacgtagt gcgccgccag cacgccttcg
     1921 agcctcgcgt tgaagcccac aacctcgccg tccggattgt cgcgatactg ccagcgcagc
     1981 gcatcctcgg agaagtgacg cgcatcgggg aacaccccgc gcaacag
//
LOCUS       DAOQOC010000552         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37006_length_2026_cov_3.740741, whole genome shotgun sequence.
ACCESSION   DAOQOC010000552 DAOQOC010000000
VERSION     DAOQOC010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37006_length_2026_cov_3.740741"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PL106_09225"
     CDS             <1..892
                     /locus_tag="PL106_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNA33290.1"
                     /translation="TGGSVRQPASFCGLYGFKPTYGRVSRYGLIAFASSFDQVSPFAR
                     SADDLMAVHRTIAGHDPLDNTSSARPLPPPAETTGKLRIGWYRDCVERDGVDPGIRAH
                     MLSNIERLKAQGHTVEPIELPMLDLLVPTYYILATAEASSNLARFDGIRYGHRSRTAQ
                     GVDDTYRKSRSEGFGPEVKRRILLGTFVLSAGYYDAYYAQGMRVRRLIREHTLRSFER
                     YDLIMTPTCPVTAFAHGAISDPVAMYLQDIFTVQANLTGVPAISVPTGTHANGMPFGT
                     QLMAKPFDDERLIAWAGKLF"
     gene            complement(935..1690)
                     /locus_tag="PL106_09230"
     CDS             complement(935..1690)
                     /locus_tag="PL106_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA33291.1"
                     /translation="MAQTNVLVVEDESIVSKDIQHSLKKLGYNIAGAASSGDQAVKLA
                     IETMPDIILMDIMLKGEMNGIEAATQIRAETNIPVIFLTAYADESTLNKAKVTQPYGY
                     IIKPFKEIDIHTSIEMALYKHKKEAEVLKERDLLFNLVEGQASGKDLLFVKSNSRLVK
                     LRTSDIYFVEALKDYVVINTLNMRYTIHSTMKDIESKLPEAEFLRVHRSFIVRIDKIV
                     AIEQPNLILENDKKIIPIGGSYRDELAKRLNLV"
     gene            complement(1702..>2026)
                     /locus_tag="PL106_09235"
     CDS             complement(1702..>2026)
                     /locus_tag="PL106_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA33292.1"
                     /translation="QAIPCGLILNELIGNALKHAFPDGAAGRIAIGLHADGDQVRIRV
                     TDDGRGMPPDFDHARHANLGLELVDTLIGQLDGHIERSNPAPGRSQGVSYLITFGRTG
                     LNRNI"
BASE COUNT          368 a          620 c          611 g          427 t
ORIGIN      
        1 gaccggtggt tcggtgcgcc agccggcctc cttttgcggt ttgtacggat tcaagcccac
       61 gtatggccgg gtcagccgct acgggctcat cgcgttcgcc agcagcttcg accaggtttc
      121 gcctttcgca cgcagcgccg atgacctgat ggccgtacac cgcaccatcg ccggccacga
      181 cccgctggac aacaccagca gcgcgcgacc cctgccgcct ccggcggaaa cgaccggaaa
      241 actccgtatc ggctggtacc gggattgcgt ggagcgcgat ggcgttgacc ccgggatacg
      301 tgcgcacatg ctctcgaaca tcgagcggtt gaaggctcag gggcatacgg ttgagcccat
      361 tgaactgccg atgctggacc ttctggtgcc cacctactac atcctggcca cggccgaggc
      421 aagcagcaac ctggcccgct tcgacgggat ccgttacggc caccgcagca gaacggcgca
      481 gggcgtggat gatacgtacc gaaaaagccg cagcgaaggc tttggcccgg aggtgaagcg
      541 ccgcatcctg ttgggcacgt tcgtgctcag cgcgggctac tatgatgcgt actacgcaca
      601 gggcatgcgt gtgcgcaggt tgatccgcga acacacgctc cgctccttcg agcggtacga
      661 cctgatcatg acgcctacgt gcccggtcac ggccttcgcg cacggggcta tcagcgatcc
      721 ggtggccatg tacctgcagg acatcttcac cgtgcaggcc aacctcaccg gcgtacccgc
      781 catttcggtg cccacgggca cgcacgccaa tggcatgccg ttcggcacac aactcatggc
      841 gaaaccattc gacgatgagc ggttgatcgc ttgggccggg aaactgttct gaggcggtcc
      901 tggcggagcg aaaggcaacc tccgcaccaa cctactatac gagattcagc cgcttggcca
      961 actcgtccct gtagctgccc ccgatgggaa tgatcttctt gtcgttctcc aggatcaggt
     1021 tgggctgttc aatggccacg atcttgtcga tgcgcacgat gaaggagcgg tgaacgcgca
     1081 ggaattcggc ctccggcaac ttcgactcga tgtccttcat ggtgctgtgg atcgtatagc
     1141 gcatgttcag cgtattgatg accacataat ccttcagcgc ttcaacgaag tagatatcac
     1201 tggtgcgcag cttcaccaag cggctgttgc tcttcacgaa cagcaggtcc ttgcccgagg
     1261 cttggccctc cacaaggttg aacagcaggt cgcgttcctt gagcacctcg gcctctttct
     1321 tgtgcttgta cagggccatc tcgatcgacg tgtggatgtc gatctccttg aacggcttga
     1381 tgatgtagcc gtagggctgg gtcaccttgg ccttgttcag ggtgctttca tccgcatagg
     1441 cggtgagaaa gatgaccggg atgttggtct cggcgcgtat ctgagtggcg gcctcgatgc
     1501 cgttcatctc gcccttgagc atgatatcca tgaggatgat gtcgggcatc gtctcgatgg
     1561 ccagcttcac agcctggtcg ccactgctcg cagcgccggc tatgttgtat cccagcttct
     1621 tcaggctatg ctggatatcc ttgcttacga tgctttcatc ctcaaccacc aggacgttcg
     1681 tctgggccat tgctcggggg cttaaatgtt cctgttcagg ccggtccggc cgaaggtaat
     1741 caaatacgag accccttggc tccggccagg ggcggggtta ctacgctcaa tatgcccgtc
     1801 aagttgcccg atcagggtgt ccaccagttc gaggcccagg ttggcatggc gcgcatggtc
     1861 gaaatccggt ggcattccac ggccgtcatc ggtcacccgg atccgcacct gatccccgtc
     1921 ggcatgcagg ccaatggcga tacgaccggc ggccccgtcg gggaaggcgt gtttgagggc
     1981 gttcccgatg agttcgttga gaatgagtcc gcaggggatg gcctgg
//
LOCUS       DAOQOC010000553         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37044_length_2025_cov_3.218782, whole genome shotgun sequence.
ACCESSION   DAOQOC010000553 DAOQOC010000000
VERSION     DAOQOC010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37044_length_2025_cov_3.218782"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..546)
                     /locus_tag="PL106_09240"
     CDS             complement(88..546)
                     /locus_tag="PL106_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNA33293.1"
                     /translation="MKKLIATLGLSALVLGAFAQEGKVVGGSGPMISMDKEVHDYGTI
                     DKGANGTCEFKVTNTGDAPLIISSCKGSCGCTVPKCDTAPVAPGQSTIVTVKYDSNRV
                     GPINKSVTISSNAANAPEKIVRIKGEVKDTGTTAPTSPVKEVSPMAPTNP"
     gene            717..>2025
                     /locus_tag="PL106_09245"
     CDS             717..>2025
                     /locus_tag="PL106_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNA33294.1"
                     /translation="MNLLAEQDGMSRWWRIVKESLAGEERDWTAMNVRRAIVLLSIPM
                     ILEMGMEAVFALVDIFFVSRLGEEAIAVVGLTELLLTFVYSVAWGLGMGITSVVARRT
                     GEKDPGGAAQAAMQGLLLTIVLGVAMAIPGVLFPQELLGLMNAEEAVQELGSGYVRLM
                     FGSNLIILLLFSINAVFRGAGDAAMAMRTLMLANGINIVLCPIMIHGIGGWEGFGVTG
                     AALATTIGRGTGVVYQVMRLFGKRSGINLRGVPFALRPAVMWTIVRVSLGGVVQFILP
                     SISWMFLGSIVATFGSAATAGYTVAVRIMIFSLLPSWGMANAASTLVGQNLGAGQPDR
                     AQRSAWLCGHFNMVYLVCVAVFYWLFAPVLVGFFSEGDVPAGYGVSALRILCLGYFFY
                     GYGMVLAQAFNGAGDSLTPTWMNLVCFWCMEIPLAWLLAVGVRI"
BASE COUNT          290 a          556 c          726 g          453 t
ORIGIN      
        1 ggaacgggaa ttggcgctgc gaaggtggtg gaaaagaaga agccccctgc cgcgtggcag
       61 agggctccgt tgcctgatgg cgttcgctca ggggttggtg ggggccatgg ggctcacctc
      121 cttcaccggg ctggtggggg cggtggtgcc ggtgtccttc acttctcctt tgatgcgcac
      181 gatcttctcg ggggcgttgg cggcgttgct gctgatggtc acgctcttgt tgatggggcc
      241 tacgcggttg ctgtcgtact tcacggtaac gatggtgctc tggccgggcg ctacgggggc
      301 ggtgtcgcac ttcggaacgg tgcaaccgca gctgcccttg caggagctga tgatcagggg
      361 ggcgtcgccg gtgttcgtca ccttgaactc gcaggtgccg ttggcaccct tgtcgatggt
      421 gccgtagtca tgcacctcct tgtccatgct gatcatgggg ccggagccgc cgaccacttt
      481 gccctcttga gcgaaggcgc cgagtaccag ggcgctgagg ccgagggtcg cgatgagctt
      541 tttcatgaga tccgttgagt tggtttcgtt ggttgaacgg gccaaaagta gaggggtgtt
      601 tcgcggaggg cccggcttta accgaagatt aacagcctgg gtacctccac ctttaccaag
      661 gtccggcgtg catggcaatt gacgtgccgg accacttttg cgacggatgg tacgtcatga
      721 acctccttgc agaacaggac gggatgagca gatggtggcg catagtgaag gagtcgctcg
      781 cgggcgagga gcgcgactgg acggctatga acgtgcgccg cgccatcgtg ctgctgagca
      841 ttcccatgat cctggagatg ggcatggagg cggtgttcgc actggtggac atcttcttcg
      901 tcagccgcct gggggaagag gccatcgcgg tggtgggcct caccgaattg ttgctcacct
      961 tcgtgtattc cgtggcctgg gggttgggca tgggcatcac cagcgtggtg gcgcggcgca
     1021 cgggcgagaa ggatcccggg ggagcggcac aagcggccat gcaggggctg ctgctcacca
     1081 ttgtgcttgg ggttgccatg gccattcccg gtgtgctctt tccgcaggag ttgctgggtc
     1141 tgatgaacgc tgaagaggcg gttcaggagt tgggcagcgg ttacgtacgg ctgatgttcg
     1201 ggagcaacct gatcatcttg ctgctcttct ccatcaatgc ggtgttccgt ggtgcgggcg
     1261 atgcggccat ggccatgcgc actttgatgc tggccaacgg gatcaacatc gtcctctgcc
     1321 cgatcatgat ccacggtatc ggcggttggg agggcttcgg cgtaacaggt gcggcgcttg
     1381 ccaccaccat cggtcgcggc accggggtgg tgtaccaggt gatgcgcctg ttcgggaagc
     1441 ggagcggcat caacctgcgg ggcgtgccct ttgcgttgcg gcctgcggtg atgtggacca
     1501 tcgtgcgggt atcgctcggg ggcgtggtgc aattcattct gccctcgatc agctggatgt
     1561 tcctgggttc catcgtggcc accttcggca gtgcggccac ggccggatac accgtggcgg
     1621 tgcgcatcat gatcttctcc ctgctgccct cttggggcat ggccaacgcg gccagcacgc
     1681 tggtggggca gaacctgggg gcgggacagc cggaccgtgc gcagcgatcg gcctggctct
     1741 gcggccactt caacatggtg tacctggtgt gcgtggcggt gttctattgg ctgttcgcgc
     1801 cggtactggt gggcttcttc agcgagggcg atgtgcccgc tggctacggt gtgtccgcct
     1861 tgcggatcct ttgtctcggg tatttcttct acggctacgg catggtgctg gcgcaggcct
     1921 tcaacggtgc gggcgacagc cttacgccca cctggatgaa cctggtgtgc ttctggtgca
     1981 tggagatccc gctggcctgg ctgctcgccg tgggcgtccg catca
//
LOCUS       DAOQOC010000554         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37111_length_2023_cov_3.225102, whole genome shotgun sequence.
ACCESSION   DAOQOC010000554 DAOQOC010000000
VERSION     DAOQOC010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37111_length_2023_cov_3.225102"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..468
                     /locus_tag="PL106_09250"
     CDS             148..468
                     /locus_tag="PL106_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33295.1"
                     /translation="MTPRPTHQRTPHILSTASNLLGFCLIVQTSMRLGGGLERTFVDE
                     LTALTSLVLMVTCILSFLSLRSHNTRRSELYERVADLLFIAALFTIFVVIAVIAADKG
                     LLSH"
     gene            475..>2023
                     /locus_tag="PL106_09255"
     CDS             475..>2023
                     /locus_tag="PL106_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA33296.1"
                     /translation="MAATPPTDARTSSPWSLVVPAILQDRRKFILVVALAVLSTGATL
                     VEPIVYRMAVNDVSGLFVDQAVRATKNRYDIDQDRAIPVHKEAHHRGHVARRTPEQVW
                     PTLLWAVIILFAVNIVGKILWWVGDNQNVRLTCIVERRYLENAFGHVLRLPLRFFGER
                     SSSAIVKRIDQTEQLTEIMNGFSQQVLPEVISLVGILAIMLSQNVVLTLLSLSVVPFY
                     LWLAWRSSKHLETGLDAYYEHWEAVSARMQDAVSGIKTVKLSGAEQREVNALRVSSDK
                     AYGNYIQRSLLVNKYTFWETALTYVVSALVLAYGGWLALDHKLTPGDVVMFVAFIDRL
                     YDPIDSLSSLWVDMQQAAASLARSQRLLGNVMEEPVGQPFEVVQGSVEFRDVHFGYKP
                     EREVLKGLSFRVAPGTKVALVGGSGAGKTTTVDLLLKLFAPWSGDILVDGRSILNSDP
                     AAVRRSIGMVMSDGMVFRGSLMDNIRYGRPDAAETEVLDAAIAAGMAHTLERLPNGLR
                     TEVGEGGM"
BASE COUNT          362 a          669 c          607 g          385 t
ORIGIN      
        1 tgtcccagcc gttggaccat tccgaagcga aggcgcaagg cggaccggtg aacgggctat
       61 gatccggcca gctgttccgg cccaggcagg tactcatcag atcgccacac acaacgatag
      121 gccacccatc gccataccac ccggcacatg acgccccgcc ctacccacca acgcacaccc
      181 cacatcctca gtacggcgag caacctgctg ggcttctgcc tgatcgtaca aacatccatg
      241 cgtctgggtg gtggcctgga gcgcaccttc gtggatgaac tgaccgcgct cacctcgctg
      301 gtgctcatgg tgacctgcat cctttccttc ctttcgctac gctcgcacaa cacccggcga
      361 agcgagttgt acgagcgcgt cgccgacctg ttgttcatcg ccgcgctgtt caccatcttc
      421 gtggtcatcg ccgtgatcgc ggcggacaag ggactgttgt cccactgagc gcaaatggcc
      481 gccacccccc cgaccgacgc gcgaacgtcc agtccctggt ccttggtggt accggccatc
      541 ctgcaggacc ggcgcaagtt catcctggtg gtggcattgg cggtgctcag caccggggcc
      601 acccttgtgg aaccgatcgt gtaccgcatg gcggtcaacg atgtgagcgg cctcttcgtg
      661 gaccaggccg tgcgcgcgac caagaaccgc tacgatatcg accaggaccg ggcgataccc
      721 gttcacaagg aggcgcacca tcgcggccac gtggcccgtc gcacgccgga acaggtatgg
      781 cccaccctcc tttgggccgt gatcatcctc ttcgccgtga acatcgtggg caagatcctc
      841 tggtgggtcg gcgacaacca gaacgtgcgc ctcacctgca tcgtggaacg acgctacctg
      901 gagaacgcct tcgggcatgt gctccggctg ccgctgcgat tcttcggtga gcgcagcagc
      961 tccgccatcg tcaagcgcat cgaccagacg gaacagctca ccgagatcat gaacgggttc
     1021 tcccagcaag tgctgcccga ggtgatcagc ctcgtgggca tcctcgccat catgctctcc
     1081 cagaacgtgg tgctcaccct gctctccctt tccgtggtgc cgttctacct ctggctggcc
     1141 tggcgcagca gcaaacacct ggagaccggc cttgatgcct actacgagca ttgggaagcg
     1201 gtgtccgccc gcatgcagga tgccgtttcc gggatcaaaa ccgtgaagct cagcggtgct
     1261 gaacagcgcg aggtcaacgc cctgcgtgtc tcgtcggaca aggcctacgg gaattacatc
     1321 caacgcagct tgctggtgaa caaatatacc ttctgggaga ccgcattgac ctatgtggtg
     1381 agcgcactcg tactcgccta tggcggctgg ctggccctgg atcacaaact cacgcccggt
     1441 gacgtggtca tgttcgtggc cttcatcgac cggctttacg acccgatcga ctccctcagc
     1501 tcgctgtggg tggacatgca acaggcggcc gcatcgctcg cgcgttcgca gcgcttgctc
     1561 ggcaacgtga tggaagaacc tgtcggccag ccgttcgaag tggtacaggg ttcggtagaa
     1621 ttccgcgatg ttcacttcgg gtacaaaccc gagcgcgagg ttttgaaggg gctttccttc
     1681 cgtgttgcac ccgggaccaa ggtggccctg gtaggcggta gcggcgcggg caagaccacc
     1741 acggtggacc tgcttctcaa gctcttcgcc ccgtggagcg gcgatatcct ggtggatggc
     1801 cggtccatcc tcaacagcga ccccgctgca gtgcgccggt ccatcggcat ggtcatgtcc
     1861 gacggcatgg tgttccgtgg ttcgctcatg gacaacatcc ggtacggcag acccgacgcg
     1921 gcggagaccg aggtgctcga tgcggccatc gccgcgggca tggcccatac gcttgagcgc
     1981 ttgcccaatg gtcttcgcac cgaggtgggc gagggtggca tgg
//
LOCUS       DAOQOC010000555         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37281_length_2018_cov_4.046358, whole genome shotgun sequence.
ACCESSION   DAOQOC010000555 DAOQOC010000000
VERSION     DAOQOC010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37281_length_2018_cov_4.046358"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..546
                     /locus_tag="PL106_09260"
     CDS             394..546
                     /locus_tag="PL106_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33297.1"
                     /translation="MAGREAMRFAVGAFGEEIVLDVRGQVPGAYVLQFISANRIMAIS
                     HVVIER"
     gene            613..1488
                     /locus_tag="PL106_09265"
     CDS             613..1488
                     /locus_tag="PL106_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33298.1"
                     /translation="MFGALWLCGSDASAQSWCPPGAHWLFDTGSPWSTVQTLRTYAQD
                     TVIDGYTAQRIDYSFRLIEFGGIDTLIEAIGTAGYTRYEEEVVYERVDGGWDTLYWFG
                     AVPGDEWAPSEPVENICGQVFHVLDTSSTIIDGVQLRVVSGEMREAGASIEVPFSITE
                     RIGGGWGGSIFPWLPTCGIIECYCALLCYSDQEIATSPGGECEITLSAAEAGGNTVQR
                     LFPNPGTDRFTIQWNTPDPTTIQVRDAMGRIVSTGRYTNTSITVDASAWPPGLYHVVV
                     SDVAGAVRVMKWLKE"
BASE COUNT          403 a          565 c          634 g          416 t
ORIGIN      
        1 ccgcagtgat acggcgggac cctgcgttca gatccacggt gacccgaccg gcgaaaacag
       61 gggcgcaagg tgcgtacgcc taccttcgat atcgacgagg aggcgttgcg gatcggaagt
      121 gcgatgatag cgtgggcggc ggtgggggag ttgggggcgt gagttgttcg gcgccagtcg
      181 aacctcctgg ctaccatata cgttgaatat ttcaaacaaa atacttgttt gttcctgttc
      241 tgctcgtgtg cttcgtggtg tgcaacactg aaaccccatt cgccatgagc acaaaggaca
      301 cgaacacggg aacgatggtg gcgcgagtcg cgcaaggttt tcaacaggtg ttgataaaag
      361 ggggcgacca atgttcaatt gactcgtcct gaaatggcgg gccgcgaggc gatgcgtttc
      421 gctgtcgggg ccttcggcga ggagatcgtg ctggatgtac gcgggcaagt cccgggtgcg
      481 tacgtcctcc aattcatctc ggcgaacagg atcatggcaa taagccatgt ggtgatcgaa
      541 cgataggccg aacaacaccc gataacaaag atgaaacacg ctatccattc atctttgcct
      601 tgcgcgtctt tcttgttcgg cgctctatgg ctctgcggat cggatgccag cgcccaaagc
      661 tggtgcccac ccggagcgca ttggctattc gacacgggca gcccctggag taccgttcaa
      721 acgctgcgca cttatgccca agacacggtc atcgatggat acaccgcaca gcgtatcgac
      781 tattccttcc ggctgatcga gttcgggggt attgacacgc tgatcgaggc gatcggcacg
      841 gctggttaca cgcgctacga agaagaggtg gtctatgaaa gggtcgatgg cggatgggac
      901 accttgtact ggttcggtgc ggtgcctgga gatgagtggg cgccatccga gccggtcgag
      961 aacatctgcg ggcaggtgtt ccatgttctg gacacgagca gcacgatcat tgatggtgtt
     1021 caactccgcg tggtttccgg tgaaatgcgc gaagcgggcg cttcgatcga ggtccccttt
     1081 tcgatcaccg aacgcattgg cggcggatgg ggcggatcga tattcccctg gttgcccaca
     1141 tgtgggataa ttgagtgcta ttgtgcgctg ctgtgctact cggaccagga gatcgccacg
     1201 tcccctggtg gtgaatgcga gatcaccttg agcgccgccg aagcgggcgg caataccgta
     1261 cagcggttat tccccaatcc cggcacggat cggttcacca ttcaatggaa cacacccgac
     1321 cccaccacca tccaggtacg cgatgccatg gggcggatcg tgagtacggg ccggtacacc
     1381 aacacgtcga tcacggtgga tgcttcggcc tggccaccgg gtttgtacca tgtggtggtg
     1441 agcgatgtgg ccggggcggt gcgcgtgatg aaatggttga aggagtaggg gttggtcgcc
     1501 atcgtccagc aggaccatag tgtcatgtca cgcttcggct ggctcacgct tcagcaggct
     1561 tacgcttcgg caggtctctc tgatcatctt ggtcgatcgg gacagggatc cacccacgag
     1621 tcttcggcta ttcagcggag aagcagccac cggaccacgg cccgaacgcc gaggacgaaa
     1681 gccccgacgc gcatctcctc cttgaacagg tgcaggtcgg gcatccatat ggcaggtgtt
     1741 cctgtagctg cgtggaacgc gccagaggcg cacacctcac acgtccgcgc tacaagcgcg
     1801 gaccggcacg gaccacaacg ttgtgaaggc cgccatcccg atcgatccga agaagaaggt
     1861 tctcggctca gttcgccacc tcgctcagga acttcaggcg catgaagcgg atctcgtcct
     1921 cgctgtagtc gccgccgaac tcgtcgtggg cctgcttcag gtcgtcgctt tcggcctcgc
     1981 ggaagtagtc catgatctcc tcctgctggt cgggatcg
//
LOCUS       DAOQOC010000556         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37327_length_2017_cov_2.937819, whole genome shotgun sequence.
ACCESSION   DAOQOC010000556 DAOQOC010000000
VERSION     DAOQOC010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37327_length_2017_cov_2.937819"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1525)
                     /locus_tag="PL106_09270"
     CDS             complement(191..1525)
                     /locus_tag="PL106_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNA33299.1"
                     /translation="MSNALRSFFLTSRFFGIGWGIVALFCAAFPFPFLLPVATGLLVA
                     LAAVTLVDTLLLHRVRSGINASRNTLDKWSNGDENPVTITVTSRYGIPVDVRVLDELP
                     FQFQERSLDLHARIPPGGTRELNYMVRPVERGVYTYGAINILVRTRLGLAERRYRQEQ
                     GKEVATYPSYIHLRKYELMALADRWSMAGVKKVRRSAQRVEFERIRNYVPGDDKRTVN
                     WKATARRGELMVNQYQDEKAQQIISLIDTGRTMKMPFEGLTLLDRAINASLVLSDIAI
                     KKDDKAGVITYSDKVHNLLPPGRDKTQIKKILELLYAQRTDFAETDFDALFARARRAL
                     GQRSLLVLYTNFESLAAMRRQLPFLQRLARAHLVAVVIFRNTELDELLTLPDRDTVDV
                     YVKTITGKLMHEKRLIAKELESHGVLTILTRPQDLSVNVLNKYLEIKARALI"
     gene            complement(1522..>2017)
                     /locus_tag="PL106_09275"
     CDS             complement(1522..>2017)
                     /locus_tag="PL106_09275"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA33300.1"
                     /translation="EHEGTYRLPEAQLDRFLFKGEIGYPGVDDEVALLQRSQEGADRM
                     STAHIRPTISPAELLHARTLAAGVRCDEKLVRYIAEIIHATRNDRTLMLGASPRASLA
                     TLRASKSAAAIQGRDFVVPEDIQLVLPPVLRHRILLTPEREMEGLRPDEVVAQFIAKV
                     PVPR"
BASE COUNT          320 a          596 c          678 g          423 t
ORIGIN      
        1 cgatcccctt caccagggtt gtcttgcccg tctgtcgtgc accgttcagc agcaccaccg
       61 gcgtgtcctt cagcgcgatg tcgatgtagt actggatatt cctggtcctc atggcgggcc
      121 tccgttgccg gtagctcgca cgaaggtagc ccgatcatgg aaaattcgcc acgaagtggc
      181 gaatttccga tcatatcaac gcccgtgcct tgatctccag gtacttgttc agcacgttca
      241 cgctcaggtc ctgcggcctg gtgaggatgg tgagcacgcc gtggctctcg agttccttgg
      301 cgatgagtct tttctcgtgc atcaacttgc cggtgatggt cttcacgtac acgtccacgg
      361 tgtcgcggtc gggcagggtg agcagttcgt ccagttcggt gttgcggaag atgaccacgg
      421 ccaccaggtg ggcacgtgcc agtcgttgca ggaacggcaa ctgcctgcgc atggccgcga
      481 ggctctcgaa attggtgtac agcaccagca ggctgcgctg gcccagtgcg cgtctggccc
      541 gggcgaagag cgcgtcgaag tccgtctctg cgaagtccgt tcgctgggcg tacaggagtt
      601 cgagtatctt cttgatctgc gtcttgtcgc ggcccggagg aagcaggttg tgtaccttgt
      661 cgctgtacgt gatgacgccg gccttgtcgt ccttcttgat cgcgatgtcg ctcaggacca
      721 gcgacgcgtt gatggcgcgg tccagcaggg tgagtccttc gaagggcatt ttcatggtgc
      781 ggcccgtgtc gatcaggctg atgatctgct gggccttctc gtcctggtac tggttcacca
      841 tcagttcgcc gcgccgggcc gtggccttcc agttcacggt ccgtttgtcg tcgccgggca
      901 cgtagttccg gatgcgctcg aattccacgc gttgggcgct gcgacgcacc ttcttcacac
      961 cggccatgct ccagcggtcg gccagggcca tcaattcata cttgcggagg tggatgtagc
     1021 tggggtaggt ggccacttcc tttccctgtt cctggcggta acgccgctcg gccagtccca
     1081 ggcgggtgcg caccaggatg ttgatggcgc cgtaggtgta cacaccgcgt tccacgggcc
     1141 gcaccatgta gttcagttcc cgtgttcccc cgggcggtat ccgcgcatgc aggtccaggc
     1201 tacgttcctg gaattggaag gggagttcat ccagcacgcg gacatccacg ggtatgccgt
     1261 agcggctcgt cacggtgatg gtcacggggt tctcatcgcc gttgctccat ttgtccagcg
     1321 tgttgcgcga tgcgttgatg ccggacctca cgcgatggag caggagggtg tccacgagtg
     1381 tgaccgcagc gagcgcgacc aggagcccgg tggccacggg cagcaggaac ggaaagggga
     1441 acgcggcgca gaagagcgcc acgatgcccc atccgatccc gaagaatcgg gaggtgagga
     1501 agaacgatcg cagggcgttg ctcaccgggg cacgggcact ttggcgatga actgcgccac
     1561 cacctcgtcg ggccgaaggc cttccatttc gcgttcgggg gtcagcagga tccggtggcg
     1621 cagcacgggc ggcaggacga gttggatgtc ctcgggcacc acgaagtcgc gcccttggat
     1681 ggccgctgcg ctcttggaag cgcgcaaggt ggccaggctg gcccgtggcg aagcgccgag
     1741 catcaaggtg cggtcgttgc gcgtggcgtg gatgatctcc gcgatgtatc gaacgagctt
     1801 ctcgtcgcaa cgcacgccag cggccagtgt gcgggcatgg agcagttcag cgggcgagat
     1861 cgtcggtcgg atgtgagcgg tgctcatgcg gtcggcacct tcttggctcc gttgcaacag
     1921 ggccacctcg tcgtccacgc cgggatagcc gatctccccc ttgaagagga agcggtcgag
     1981 ctgtgcttcg gggaggcgat aggtgccttc atgttca
//
LOCUS       DAOQOC010000557         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37389_length_2015_cov_4.192857, whole genome shotgun sequence.
ACCESSION   DAOQOC010000557 DAOQOC010000000
VERSION     DAOQOC010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37389_length_2015_cov_4.192857"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1543
                     /locus_tag="PL106_09280"
     CDS             260..1543
                     /locus_tag="PL106_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNA33301.1"
                     /translation="MPDWIALGREGRSQALLRLAALVERDIDAFARAESRDNGKPVAL
                     AREVDIPRAIANLRFFATAILHWSSEAHLMDDEAVNWTDRRPVGVAGCISPWNLPLYL
                     FTWKIAPALAAGCAVVSKPSEITPFTAFMLAERAIEAQLPSGVLNIVHGLGPKVGAAI
                     TAHPRIPAISFTGGTRTGAEIARTAAPMFKKLSLELGGKNPVLVFADCDFDRMLNETV
                     RSSFRNQGQICLCGSRILIERSIYVRFRDAFVARAKALRTGDPADPATDQGAVVSRAH
                     MDKVLGHIATARDEGGTILCGGDRVVLPGALKDGWYIAPTVIEGLGPQCRTNQEEIFG
                     PVVSLQPFDTEDEALALANATPYGLASVLWTSDVKRAHRVSRALNSGIVWVNCWMLRD
                     LRTPFGGMKQSGVGREGGTEALRFFTEAKNVCMAY"
     gene            complement(1569..>2015)
                     /locus_tag="PL106_09285"
     CDS             complement(1569..>2015)
                     /locus_tag="PL106_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33302.1"
                     /translation="DLFTLAVRPGDARGQETVSGAMAIHALVAQHGISLVHAGAMAGD
                     VRDAVRGMLEDGADDGGHHRGEAFIRALALLDGAPGPSGPGDGSGVRYLCVGIRKAGQ
                     DELVVGRVMAARTAWLLAEAAKNTGSEEVAVLPDNGDDAVLLVKAL"
BASE COUNT          364 a          683 c          631 g          337 t
ORIGIN      
        1 ggtaggcatc gccaagaaag gtgggtcaaa acgaccgacc caccctacaa gactacaatt
       61 cgtcatggga gcggatgcgt tggcggacaa atctccgatc gacctgaact acgccgacgc
      121 cgaggcgctg acgtcagacc cacggtacga accacaactt cgccgcatcc ggcggcgcgg
      181 actattgact ctattgtcga tattgatatt tctcccgacg gggttgggcg ctgccgcgct
      241 gcagaagcgc tttccggggc tgcccgattg gatcgccttg ggtcgtgagg gccgcagcca
      301 agcactgctc cgcctggctg cgctggtgga gcgcgacatc gatgcgttcg cccgggccga
      361 aagccgcgac aacgggaagc ccgtggcctt ggcgcgggag gtggacattc cgagggccat
      421 cgccaacctg cgcttcttcg ccacggccat cctccattgg agcagtgaag cgcacctcat
      481 ggacgacgag gccgtgaact ggacggatcg tcgacccgtg ggcgtggccg ggtgcatcag
      541 cccgtggaac ctgccgctct atctcttcac gtggaagatc gcacctgccc tggccgctgg
      601 gtgcgcggtg gtatcaaaac cctcggagat cacgccgttc accgcgttca tgctcgccga
      661 acgcgcgatc gaggcgcaac tcccatccgg ggtactcaac atcgtgcatg gcctgggacc
      721 caaggtgggc gccgccatca ccgcgcaccc gcgcataccg gccatctcgt tcaccggtgg
      781 tactcgtacc ggggccgaga tcgcccgcac cgccgcaccc atgttcaaga agctcagcct
      841 tgagcttggt ggcaagaacc cggtgctcgt attcgccgat tgcgactttg accggatgct
      901 caacgaaacg gtgcgctcgt cgttccgcaa ccagggccag atctgcctgt gcggctcgcg
      961 tatcctgatc gaacgttcca tctacgtgcg cttccgcgat gccttcgtgg cgcgggccaa
     1021 ggcgttgcgt acgggcgacc cggcggaccc cgcgaccgac cagggcgccg tggtgagccg
     1081 ggcgcacatg gacaaggtgc tcggccatat cgccaccgcc cgggacgagg gcggaacgat
     1141 cctctgcggc ggggatcgtg tcgttctccc cggggccttg aaggacgggt ggtacatcgc
     1201 gccaacggtg atcgagggac tcggcccgca gtgccgcacg aaccaggagg agatcttcgg
     1261 gcccgtggtc tcattgcaac cattcgatac cgaggacgaa gcattggcac tggccaacgc
     1321 caccccgtac ggactggcca gcgtgctgtg gacatccgat gtgaaacgcg cccaccgcgt
     1381 gtcgcgtgcg ctcaacagcg gcatcgtgtg ggtgaattgc tggatgcttc gcgacctgcg
     1441 cacacccttc ggcggtatga agcagagcgg tgtggggcgc gaaggcggta ctgaggccct
     1501 gcgcttcttc accgaggcca agaacgtgtg catggcgtat tgatgatggg gccgaacagc
     1561 ggccggtatc acaacgcctt caccaggagc acggcatcat cgccattatc ggggagcacc
     1621 gccacttcct ccgatccggt attcttcgcg gcctccgcca gcagccacgc ggtgcgggcc
     1681 gccatcacgc gtccgaccac cagctcgtcc tgccccgcct tcctgatacc aacgcaaagg
     1741 taacgtacac cactgccgtc gccgggaccc gaagggccgg gggcaccgtc cagcagcgca
     1801 agggcacgga tgaacgcttc accccggtga tgaccaccat cgtccgcgcc gtcctccagc
     1861 atcccgcgta ccgcatcgcg tacatcgccc gccattgcac ccgcatggac cagggagatc
     1921 ccgtgctgcg ccaccaacgc atgaatggcc atcgcaccgg acacggtctc ctgtcctcgc
     1981 gcgtcacccg gtcgtacggc caaggtgaag aggtc
//
LOCUS       DAOQOC010000558         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37489_length_2012_cov_4.554420, whole genome shotgun sequence.
ACCESSION   DAOQOC010000558 DAOQOC010000000
VERSION     DAOQOC010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37489_length_2012_cov_4.554420"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PL106_09290"
     CDS             complement(<1..420)
                     /locus_tag="PL106_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HNA33303.1"
                     /translation="MLFRSPRAARIVLLVLAFHASWCLSRLSAQYPPAQPDASAILHR
                     MQKLNVLGSVLYIVAHPDDENTRLITWLANGKKMRTGNLSLTRGDGGQNLIGPELGDA
                     LGIIRTQELLAARRIDGGEQFFTRAVDFGYSKNAAESF"
     gene            complement(763..1317)
                     /locus_tag="PL106_09295"
     CDS             complement(763..1317)
                     /locus_tag="PL106_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNA33304.1"
                     /translation="MAKTNDIIENPVTHERIRFLTTSEDSDGELLKIEFTCKVGAQGP
                     PEHIHPHQSETFEIINGKAGILLNGKEHFHTTGQKVIVPANAPHRFWNAGDHELLMTA
                     EFKPALRTEYFLESVFSIAEQGKANKTGTPGNIFQFGAILNEYYGETFIVGPPIIAQK
                     FLAKVIGGFGKLIGYKGFVPYNRN"
     gene            1421..>2012
                     /locus_tag="PL106_09300"
     CDS             1421..>2012
                     /locus_tag="PL106_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33305.1"
                     /translation="MHKLHTLAALLPLTALAQSWAPIGAKWTYSQGYVSSPDSNLAII
                     NAVGDTLLGGRTYTRLEVQGGWFTCHAFLPYFAANADTVWFWDTYQEQEQVLFRWDAL
                     PGESWSTPISVFENIFDTLEWTVLDTGHVQVDGSWLRQLFVEVISAQGLLDPVNQGLI
                     TERLGGSTAPFTWTLGPCDAEIFNYLRCYEDAEVQWH"
BASE COUNT          406 a          558 c          559 g          489 t
ORIGIN      
        1 gaagctctct gccgcgttct tgctgtagcc gaagtccacc gcgcgggtga agaattgttc
       61 accaccatcg atgcgccgcg ctgccagcaa ttcctgcgtg cggatgatgc ccagcgcgtc
      121 cccgagttcc gggccgatca ggttctgccc gccatcgcca cgcgtgaggc tcaagttgcc
      181 ggtccgcatc ttcttgccgt tcgccaacca cgtgatcagc cgggtgttct cgtcgtcggg
      241 atgcgctacg atgtacagca cgctgcccaa tacgttcagc ttctgcatgc ggtgcaggat
      301 cgctgaggca tcgggttgcg ctggtgggta ctgggcggac agccgggaca agcaccagga
      361 tgcgtggaat gcgaggacga gcaacacgat gcgcgcggcc cgaggggagc ggaacagcat
      421 gcgccgaatg tacacgccgg agcttggccg tgatggttta ttgccaccac tggttcatcc
      481 ttttcggcac atggcgccca ccggtcattc cgcgggtcca ctcgtgttgc tccgctgata
      541 tgcacgatca agcggacccc ggggcagcgt tctgcgggtg gatggcatca agaatgaaag
      601 cataccgttg ccggtattgc cgcagtgggg ggccttgagc aagaatgtcc aatagatgta
      661 ctgccgttcg ttcacgcaca agagtttcat tgaagcacgt cagacccatc cggcgatacc
      721 atgtgttgcg ttcgtgctac acaagtcgaa tggttggtca tttcagttcc tattatacgg
      781 aacaaaacct ttatacccga tcaatttccc gaatccaccg atcactttag caagaaattt
      841 ttgtgcgatg atcggaggac ctacaatgaa tgtctccccg taatactcgt tcagaatggc
      901 tccgaactgg aatatattcc caggggtgcc ggttttgttc gccttgcctt gttcggctat
      961 ggagaacact gattccagaa aatactctgt ccgtaatgca ggtttgaatt ccgcggtcat
     1021 caagagttca tgatcaccag cattccagaa tcggtggggg gcattcgcag gaacgatcac
     1081 cttttgtcct gttgtatgaa agtgttcttt cccgttcaat aggatcccgg ctttgccatt
     1141 tattatttca aaagtttcgg actgatgagg gtgaatatgt tcaggcggcc cctgcgctcc
     1201 caccttacac gtgaattcaa ttttcagtag ttcgccatca gagtcttccg aagttgtaag
     1261 gaatcggatc cgttcgtgag ttacgggatt ttcaattatg tcgttcgtct ttgccatggt
     1321 gggcgttgtt cagttatcga agatagatct gtttcagtct gattcatgta tttatttcct
     1381 attttcagtg ccgccaagca tgtcccaatg gcttgccgat atgcacaaac ttcacaccct
     1441 cgccgcactc ctgccgttga ccgctctggc ccaaagttgg gccccgatcg gtgcgaaatg
     1501 gacctacagc cagggatacg tcagcagccc cgacagcaac ctggccatca tcaacgcggt
     1561 gggtgatacg ctgctcggag gtcgcacgta cacacggctc gaagtccagg gaggctggtt
     1621 cacctgccat gccttcctgc cctacttcgc agcgaacgcc gataccgtgt ggttctggga
     1681 cacgtatcag gaacaggaac aggtcctctt ccgctgggat gcgctcccgg gcgagagctg
     1741 gagcacgcct atctctgttt ttgagaatat tttcgatacg ctggagtgga ccgtgctgga
     1801 tacaggccat gtgcaggtcg atgggtcgtg gttgcgtcag ctcttcgtgg aggtcatatc
     1861 tgcacagggc ctcttggatc ccgtgaacca gggcctgatc acggaacggc tcggaggatc
     1921 caccgcaccg ttcacctgga cgctcggccc ctgcgacgcg gagatcttca actacctccg
     1981 ttgttacgag gacgccgaag tgcagtggca cc
//
LOCUS       DAOQOC010000559         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37550_length_2011_cov_2.789366, whole genome shotgun sequence.
ACCESSION   DAOQOC010000559 DAOQOC010000000
VERSION     DAOQOC010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37550_length_2011_cov_2.789366"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PL106_09305"
     CDS             complement(<1..615)
                     /locus_tag="PL106_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33306.1"
                     /translation="MLRSRDELRGRLARIRAVLFDWDGVFNDGWKDIEGGSPFSEVGS
                     MGVNMLRFGLWMRHGERLPYAAIITGQHNPFAERFAQREKLHGLYMGFLNKMDAFEDF
                     LKKHDLRPEEVAFFFDDAIDLAVADRCGLRILLRGQAGAFFAEHAVSRGCVDIITALS
                     GGQNGLREATDALLTLSGHYADAMDARADLDPRYQRYLSERQRTV"
     gene            complement(674..1696)
                     /locus_tag="PL106_09310"
     CDS             complement(674..1696)
                     /locus_tag="PL106_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNA33307.1"
                     /translation="MELYLDSADPKEIEEAFKLGFLTGLTTTPTFMHRGGVKDIDKMI
                     LDLAKKAPILQVEALGETAEEVVKEAERQLKMGLKKETTVFKIPVSLEGLRACKMLRD
                     KGIKVNVHLVYTIQQAYMAMQAGASYVCPLVGRLQDQGHDALALVEQCVRAVNYYGYD
                     TRIMFSSVRTVEHVRNAVDIGVHTITVPWKLMRQLTDNHFTAIGTKQFYVDTRLITMK
                     VKDVLSRSNPIVKDSATVSEALVEMTKHGFGAVMVVDGKGKNTGVFTDGGLRRGIGQE
                     GNKFLTRKLSSLKFNAPFTISPEALLDDAQKAFKEHKVDTLSVSENGKQLGMLDIQDL
                     MKAIAG"
     gene            complement(1757..>2011)
                     /locus_tag="PL106_09315"
     CDS             complement(1757..>2011)
                     /locus_tag="PL106_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33308.1"
                     /translation="KYGIPSADPRGGNRPVMPTIARAGVAAGVDGVFIETHPDPAKAL
                     CDAASQLSMHDLEEFLKPLLDLHAVEVKHRQPVAAAAQPR"
BASE COUNT          343 a          639 c          603 g          426 t
ORIGIN      
        1 caccgtgcgc tggcgttcgg acaggtagcg ctggtaacgc gggtcgaggt cggcgcgcgc
       61 atccatggca tcggcgtaat ggcccgagag tgtgagcagg gcatccgtgg cctcgcgcaa
      121 cccattctgc ccaccactca gtgcggtgat gatgtcaaca cagccgcgtg aaacggcgtg
      181 ctccgcgaaa aacgccccgg cctgcccacg gagaaggatg cgcagcccgc agcggtcggc
      241 cacggccagg tcgatggcat cgtcgaagaa gaaggccacc tcttccggcc ggagatcgtg
      301 cttcttcagg aagtcctcaa aagcatccat cttgttgagg aagcccatgt acaacccgtg
      361 cagcttctcg cgctgggcga agcgttccgc gaacggattg tgctggccgg tgatgatggc
      421 cgcgtacggc aagcgttcgc catggcgcat ccacaacccg aagcgcaaca tgttcacccc
      481 catgcttccc acctcgctga aggggctgcc gccttcgatg tccttccaac cgtcattgaa
      541 cacgccgtcc caatcgaaaa gcaccgcacg gatacgggcc agacgacccc gcaactcgtc
      601 ccgggaacgc agcaacccgg taccgctttt caggaatggg tcccggtcgt cgggatgttc
      661 acccctgacc atcctatccc gcgatcgcct tcatcaggtc ctggatgtcc agcatgccca
      721 gttgcttgcc gttctcgctc acactcagcg tgtccacctt gtgctccttg aaggctttct
      781 gcgcatcgtc gagcagcgct tccgggctga tggtgaatgg ggcattgaat ttcagggagc
      841 tgagtttcct ggtgaggaac ttgttccctt cctgcccgat cccacggcgc agcccgccat
      901 ccgtgaagac gcccgtgttc ttccccttgc catccaccac catcaccgcg ccgaagccgt
      961 gcttggtcat ctccacgagc gcctcgctca cagtggcgct gtccttcacg atcgggttgc
     1021 tgcgcgaaag cacgtccttc accttcatgg tgatcaggcg ggtgtccacg tagaactgtt
     1081 tggtaccgat cgctgtgaag tgattatcag tgagctgtct catcagcttc cagggaacgg
     1141 tgatcgtatg cacgccgatg tccaccgcgt tgcgcacgtg ctccacggta cgcacgctgc
     1201 tgaacatgat cctcgtatcg tagccgtagt agttcacggc acgtacgcat tgctccacca
     1261 gggccagcgc gtcatgaccc tgatcctgca aacggcccac cagcgggcac acgtagctcg
     1321 cacccgcctg catggccatg tacgcctgct ggatggtgta caccaggtgg acgttcacct
     1381 tgatcccttt gtcgcgcagc attttacagg cacggagccc ctcgaggctc accgggatct
     1441 tgaagacggt ggtctccttc ttcagtccca tcttcaattg gcgctcggcc tccttcacca
     1501 cctcctcggc cgtttcgccc agcgcttcca cctgcaggat gggcgctttc ttggcaaggt
     1561 cgaggatcat tttgtcaatg tccttcactc cgccacggtg catgaacgtg ggtgtggtgg
     1621 tgaggccggt gagaaagccg agtttgaagg cttcctcaat ttccttgggg tcggcgctgt
     1681 cgagatagag ctccatggtg ttgtgttggc cgctgggatg ctgcggcgtg tggaatgcgg
     1741 tggttgtcgg ttctcctcag cggggctgtg cggctgcggc aacaggttgg cggtgtttca
     1801 cttcaacggc gtgcaggtcg agcagcggtt tgaggaactc ttccagatca tgcatgctga
     1861 gctggctggc ggcgtcgcac agggccttgg cgggatcggg gtgggtctcg atgaacacgc
     1921 catcgacgcc ggcggccacg cccgcacgtg cgatcgtggg catcaccggg cggttgccgc
     1981 cgcgcggatc cgcactgggg atgccgtact t
//
LOCUS       DAOQOC010000560         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37612_length_2009_cov_2.983623, whole genome shotgun sequence.
ACCESSION   DAOQOC010000560 DAOQOC010000000
VERSION     DAOQOC010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37612_length_2009_cov_2.983623"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2009)
                     /locus_tag="PL106_09320"
     CDS             complement(<1..>2009)
                     /locus_tag="PL106_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HNA33309.1"
                     /translation="TPGTLPSNWSNLGGDDLNWNVDNNGTNGGNMSIATGPIGDHTSN
                     NTGGKYMYVEADASGATPGKTAILQSQCWNIAGLTSPYLTFWYHMRGTQMGSLTVDVD
                     ANGSVTSNVWTQSGDHGLKWRQGWVNLAPWAGQTNLRVRFRAITGTGALSDIAIDDVE
                     VRSLTPVPGCPDPLASNYNGAVNLNDGSCAYQCPTGQKRVTIDIVNDNYAGETSWTLK
                     NSADGTLLASGTSTGTSLCVPTSTCLVFRINDSAGDGIWHNSYGYGQYWVYLDGALVK
                     QGGQFGAYEETSFNCGPGQTCATALTAQTGVVYTAPMLEYWYDWTPAATGSYTITTCG
                     LNSCDTKLWLYDFACGSINLSSGLEGSTFADDDLGGCGTQAVITANMPAGQTYHIRVG
                     TNGGSCSTASFRIDFNGAAVGCMDQNSCNFDPLATVACSGCCLPVGDPACPEGPDLTI
                     NQNALANSLSITTVNIAPSDVCAVEEGCVKGFGTRNVIRFNTRIDNIGELDYYIGSPT
                     SQPGMFDTQNCHGHAHYSGYADYLLFGQDNEPMPVGFKNGYCVIDVGCFGGTSHYGCS
                     NMGISAQCYDQYGSGTTCNWIDITDVPAGLYTLVLRTNWARRPDALGRHETNYANNYG
                     AVCINITGNPGEPRGFNVATGCTPVVDCLNQPYGSALPDCTGACN"
BASE COUNT          356 a          607 c          630 g          416 t
ORIGIN      
        1 gttgcacgcg ccggtacagt ctggcagggc actgccatag ggctggttca ggcaatccac
       61 taccggcgtg cagcccgtgg ccacattgaa acccctgggc tcccccgggt tgcccgtgat
      121 attgatgcac acggctccgt agttgttggc gtagttggtc tcgtggcgcc cgagcgcatc
      181 ggggcggcgc gcccaattgg tgcgcagaac aagggtgtag agccctgcgg gcacatcggt
      241 gatatcgatc caattgcagg ttgtgccact accatattgg tcgtagcatt gggcgctgat
      301 gcccatgttg ctgcaaccgt aatgcgaggt gccgccgaag cagcccacgt cgatcacgca
      361 gtacccgttc ttgaagccga cgggcatggg ctcgttatcc tgtccgaaaa gcaggtagtc
      421 cgcgtagccg gagtagtgcg cgtggccgtg gcagttctgt gtgtcgaaca tgccgggctg
      481 cgaagtcggg ctgccgatgt agtagtccag ctcgccgatg ttgtcgatac gggtattgaa
      541 ccggatgacg ttgcgtgtgc cgaagccctt cacacagcct tcctccaccg cacagacgtc
      601 gctcggtgcg atattgaccg tggtgatcga gagtgaattg gcgagcgcgt tctgattgat
      661 ggtcagatcc ggtccctcgg gacaggcggg atcgcccaca ggcaggcaac aaccggaaca
      721 cgctaccgtt gcgagtggat cgaagttgca tgaattctgg tccatgcatc ccaccgcagc
      781 gccgttgaag tcgatgcgga agctggccgt ggaacagctg ccaccgttgg tacccacacg
      841 gatgtgatag gtctggccgg ccggcatgtt ggccgtgatc accgcttgcg taccgcagcc
      901 gccgagatca tcgtcggcga aggtggatcc ctccagtccg ctgctcaggt tgatggaccc
      961 gcacgcgaag tcgtacaacc agagtttcgt atcgcaggag ttgagcccgc acgtcgtgat
     1021 ggtgtacgag cccgtggcag ccggtgtcca atcgtaccaa tactccagca tcggggccgt
     1081 gtacaccacc ccggtctgtg ccgtgagggc agtggcgcag gtctggccgg gcccgcaatt
     1141 gaagctcgtc tcctcgtacg caccgaactg cccaccctgc ttcaccaacg caccatcgag
     1201 gtacacccag tactgcccgt agccgtagct gttgtgccag atgccgtccc ctgccgaatc
     1261 gttgatccgg aacaccagac aggtgctcgt tggcacgcaa agggaggttc cggtggatgt
     1321 gccgctggcc agcagggtgc catcggccga gtttttcaac gtccaactgg tctccccggc
     1381 gtagttgtca ttcacgatgt cgatggtgac gcgcttctgg cctgttgggc actgatacgc
     1441 gcacgaaccg tcgttcaagt tcaccgcacc gttgtaattg ctggccagcg gatccgggca
     1501 gccgggcaca ggggtcaggc tccgcacctc cacatcgtca atggcgatat cgctgagcgc
     1561 accggtaccg gtaatggccc tgaagcgcac gcgcaggttg gtctgtccgg cccatggggc
     1621 aaggttcacc caaccctgcc gccacttcaa cccgtggtcg ccactttggg tccagacgtt
     1681 gctggtcacg ctcccattgg catccacatc cacagtcagg gaacccatct gtgtgccgcg
     1741 catgtggtac cagaaggtga gataaggtga agtgagtccg gcgatgttcc agcactgact
     1801 ctgcaaaatg gccgttttgc ccggcgtggc gccggaggca tcggcctcca cgtacatgta
     1861 tttcccgccg gtattgttgc tggtgtgatc gccgatgggg ccggtggcga tgctcatgtt
     1921 gcccccgttc gttccgttgt tgtccacgtt ccaattcagg tcatcgcccc cgaggttgga
     1981 ccagttggac ggaagggtcc ccggggtgc
//
LOCUS       DAOQOC010000561         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37649_length_2008_cov_3.378392, whole genome shotgun sequence.
ACCESSION   DAOQOC010000561 DAOQOC010000000
VERSION     DAOQOC010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37649_length_2008_cov_3.378392"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PL106_09325"
     CDS             complement(<1..451)
                     /locus_tag="PL106_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33310.1"
                     /translation="MIGKWKWSIALASVALIGTYIYVGVDVHPHEEYVEDPVAASAEE
                     DTVPAPLGAYGIPLDGFVMERGQVKSGQTFSDLLAPFGIGPSEVDSLVNMAAPVFDVH
                     KLRAGHPYAVIFTDDPAKTPAFFVYEADQVEHIIFTLQPPMDVRGLAP"
     gene            complement(536..1711)
                     /locus_tag="PL106_09330"
     CDS             complement(536..1711)
                     /locus_tag="PL106_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33311.1"
                     /translation="MNNIATDQEAWFVIHDPVTGCRRMHKIYVHAVDVGMLEFSVNEL
                     LPDCPAGSATGAAEVSGVVPAYGSTWSTVWIQNGEPIASGDQLNNVISGVYQVTVEDG
                     AGCARSSTLNIGTCQGEGEVEVIEEVVHPACPGGKDGWIRLSVEPGQEFEVLWSDGGW
                     EHERGELEAGVYSYTVHLTETDIYIEGSVELISAPFEVRIIWPGMHMIPEECIGPACA
                     AITFGPWPEELPVEEIMELVAQGWPGYEQPGQYTRDELQVQWNDDQVGLCVDQVCGHP
                     ISVSVRDPLECVVIAEAGGNDRNKHVVRDVPVAMRLFPNPNRGDVVNVTFHGLDTEEH
                     RCLLEVMDVHGRVVQRSMEIFQGRSVKSITLSTVSGVYNVRGILDGEIYTHQLIVTP"
BASE COUNT          423 a          648 c          510 g          427 t
ORIGIN      
        1 ggggcgccag gccgcgcaca tccatgggcg gctgcaacgt gaagatgatg tgttccacct
       61 ggtcggcctc gtacacgaag aaggccgggg tcttcgccgg gtcgtcggtg aagatgaccg
      121 cgtagggatg gcccgcccgc aacttgtgta catcgaatac cggtgccgcc atattcacca
      181 ggctgtccac ttcggacgga ccgataccga acggggcgag cagatcactg aaggtctgac
      241 cactcttgac ctggccacgc tccatcacga aaccgtccaa ggggatgccg tacgcgccca
      301 atggtgcggg caccgtgtcc tcttcagcgg atgctgccac gggatcttcc acgtactcct
      361 cgtgcgggtg tacgtcgacg cccacgtaga tatacgttcc gatcagggcc actgaggcca
      421 gggcgatgct ccacttccat ttcccgatca tggccgcaag aaaggcccgg gccttccgtg
      481 ccctgccgac ccgggtcccg tttctatcaa cggtgctggc ttggtaacca agcactcaag
      541 gtgtgacgat gagctgatga gtatagatct ctccgtcaag aatcccgcgt acgttgtaca
      601 cccctgaaac cgtggacaat gtgatcgatt ttacgcttcg accctggaaa atctccattg
      661 accgctggac cacccggcca tggacgtcca tcacctcgag cagacaccga tgttcctcgg
      721 tgtcaaggcc atggaacgtt acattcacca catccccacg gttcggattt gggaataagc
      781 gcatcgcaac aggaacatcc cgtaccacgt gcttattcct gtcattgcca ccggcctcgg
      841 ctataaccac gcactccagc gggtcgcgca ccgaaacaga aatgggatgt ccgcagacct
      901 gatcgacgca caaccccacc tgatcatcgt tccattgaac ctggagttca tcacgcgtat
      961 attgcccagg ctgttcgtat ccaggccagc cttgggcgac caattccatg atctcttcca
     1021 cgggcagttc ctctggccat ggaccgaagg tgatcgcggc acacgccggc ccaatgcatt
     1081 cttccggtat catgtgcata cctggccaaa tgatgcgtac ctcgaaagga gcgctgatca
     1141 gttcaacact tccttctatg tagatatccg tctcggtcag atgaacggta tagctgtaca
     1201 cacctgcttc aagctcgccc cgttcgtgtt cccatccacc atcggaccac aggacctcga
     1261 actcttgtcc aggctccact gagaggcgga tccacccatc cttgcctcca ggacaggcag
     1321 ggtgtaccac ctcttcgatc acctccacct caccttcccc ttggcacgtt ccgatgttca
     1381 gggtactgct ccttgcacaa ccagcaccgt cctcgacggt cacttgatag acgccactga
     1441 tcacgttgtt caattggtcc ccgctggcga tcggttcacc gttctgaatc caaaccgtgg
     1501 accatgtgga accataggcc ggcacaaccc ccgacacttc tgccgcaccc gttgccgatc
     1561 cggccgggca atccgggaga agctcgttca cagagaactc aagcataccc acatccaccg
     1621 cgtgcacata gatcttgtgc atgcgtctac atcccgtaac ggggtcatga atgacgaacc
     1681 acgcttcttg gtccgtggcg atgttgttca agaatgcgtt cccatcactg tccgggtgca
     1741 aactggtctg caccggttct ccgtccggac cagaccaata ccacgtgatt cccaactcac
     1801 tgaggttcgg tgtgtttacg gggaacacga tcgtcgcgga ggcaccgttc tcaacgcata
     1861 cgaccgggtc gtattcgact gtgaacgtga aaagcgttgc ggagccgacg accacatcct
     1921 cgatgatctg ctcgcagatc ggttcctcgg ttgtttcgat caacatgcta tagttcccag
     1981 cacacaggtc cgtaaagctc gcactgcc
//
LOCUS       DAOQOC010000562         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37691_length_2007_cov_3.486168, whole genome shotgun sequence.
ACCESSION   DAOQOC010000562 DAOQOC010000000
VERSION     DAOQOC010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37691_length_2007_cov_3.486168"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..396
                     /locus_tag="PL106_09335"
     CDS             49..396
                     /locus_tag="PL106_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNA33312.1"
                     /translation="MTEPPCPHCRSAITYPTGTSQMCAECGHEWDPVQVAAEVAAASE
                     ANAIKDANGNILKDGDDVVMVKDLPVKGAPKALKAGTKVKNIKLTDGDHNIDCRIEGF
                     GAMALKSIYVRKA"
     gene            542..1186
                     /gene="plsY"
                     /locus_tag="PL106_09340"
     CDS             542..1186
                     /gene="plsY"
                     /locus_tag="PL106_09340"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNA33313.1"
                     /translation="MGETWVYAIVAMALSYLSGSIPTSVWWGKAFYGLDVREHGSKNA
                     GATNTFRVLGPSAGIPVLLIDMLKGFAPVRVLAWWSGFPDDSAQFTWLRVGMALCAVL
                     GHLYPVFAGFRGGKGVATSFGCILALHRGIAMICLGVFVLVFLLFRYVSLASLCAALA
                     FPLATFLLDTEQHFVFRGFSLAIAVLVFWTHRTNIGRLLRGEEQRMRPSHGISP"
     gene            1270..>2007
                     /locus_tag="PL106_09345"
     CDS             1270..>2007
                     /locus_tag="PL106_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33314.1"
                     /translation="MVLLLLPLALMAQGGGSRAEADALFAKQKFAEALPLYTQLVSLA
                     PGDHDLNYRLGTCIVHGRGDKEKAVGFLKYAVTSPTVPALAWYYYGRAYHLTYQFAAA
                     LDAYERYRGTADKKTLAVYPVDALELQCRNGKELLNNIKDIEVLNKVEVNEDEFFRFY
                     DLRSIGGKIVVTPEELKSGLDKKSGVVSLVYLPDKGGPIYFASYGKNGDAGRDIYRTE
                     LMPNGTFATPVKLAGYINTAQDEDFPFM"
BASE COUNT          376 a          602 c          617 g          412 t
ORIGIN      
        1 ggtcacccgg tcggatatgg ataacttcgc cgccaacacc ttccggacat gaccgaaccg
       61 ccttgcccgc actgccgatc ggccatcacg tatcccacgg gaacctcgca gatgtgcgcc
      121 gaatgcggcc acgaatggga tccggtacag gtggctgcgg aagtcgccgc tgcatccgag
      181 gccaacgcga tcaaggatgc caacggcaac atcctgaagg acggcgacga tgtggtcatg
      241 gtgaaggacc tgcccgtgaa aggtgcgccc aaagcactca aggcgggcac caaggtgaag
      301 aacatcaaac tcaccgatgg tgaccacaac atcgattgcc ggatcgaggg gttcggggcg
      361 atggcgctca agtccattta cgtgcgcaag gcataggatc cgccctgcat tcgtgttggt
      421 gtattgtcgg taactgccgg tggaccatcc ggcttcatcg ggttgcagtt ctttctttgc
      481 ggggatgaag tgtcaggaaa acaacagcaa cgggtgggcg aagacccggt ccaactgaac
      541 catgggggag acttgggtgt atgcgatcgt tgccatggcc ttgagctacc tcagtggcag
      601 catccccacc agtgtgtggt ggggcaaggc gttctacggc ttggatgtgc gcgagcacgg
      661 gagcaagaac gccggtgcta ccaatacctt ccgggtgctg ggcccgagtg cgggtatccc
      721 cgtactgctc atcgatatgt tgaagggctt cgcaccggtt cgtgtactgg cctggtggag
      781 cgggttcccg gacgattccg cccagttcac ctggttgcgc gtgggcatgg ccctctgtgc
      841 cgtcctgggg cacctttatc cggtcttcgc cggtttccgt gggggtaaag gagtagccac
      901 ttccttcggc tgcatcctgg ccctgcatcg gggcatcgcc atgatatgcc tcggagtatt
      961 cgtgctggtc tttctacttt tcaggtacgt ctccctggct tccttgtgtg ccgcattggc
     1021 ctttccgttg gccacgttct tgctggacac cgagcagcat ttcgtgttcc ggggcttctc
     1081 actggcgatc gcggtcctgg ttttctggac ccaccgcacc aacattggcc ggctgctgcg
     1141 cggcgaagag caacgcatgc gaccaagtca cggcatcagc ccatgaaacc ttccctgcga
     1201 tcgacgggtt caagcgcccc gatccccgct tggctgcggg gcggcggaat gacccgtttt
     1261 gccgggatag tggtcctgtt gctcctgcct ttggccctga tggcccaagg tggcggctcg
     1321 cgtgcggagg ctgatgccct gttcgccaag cagaagttcg ccgaggcgct gcccttgtac
     1381 acccagctgg tgagccttgc cccgggcgat catgacctca actatcgctt gggtacctgc
     1441 atcgtgcatg gtcgtggcga caaggaaaag gccgtgggct tcctcaagta cgcggtcacc
     1501 agccccactg ttcccgcctt ggcctggtat tactacggcc gcgcctatca cctcacctat
     1561 cagttcgccg ctgcgctcga tgcctatgaa cggtaccggg gaaccgcgga caagaagacc
     1621 ctggccgtat atccggtgga cgcgcttgaa ctccagtgcc gcaacgggaa ggagctgctc
     1681 aacaacatca aggatatcga ggtgctcaat aaggtggagg tgaacgagga tgaattcttc
     1741 cgcttctacg acctccgcag catcggaggg aagatcgtgg tgacgccgga agagctcaag
     1801 agcggtctcg acaagaagag cggggtcgtt tcgctggtgt acctgccgga caagggtggc
     1861 ccgatctact tcgccagcta tggcaagaac ggcgatgcgg gtcgcgacat ctaccgcacc
     1921 gaactgatgc ccaacgggac cttcgccacg cccgtgaaac tcgccggcta tatcaacacc
     1981 gcccaggacg aggacttccc cttcatg
//
LOCUS       DAOQOC010000563         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37743_length_2006_cov_3.316248, whole genome shotgun sequence.
ACCESSION   DAOQOC010000563 DAOQOC010000000
VERSION     DAOQOC010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37743_length_2006_cov_3.316248"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PL106_09350"
     CDS             <1..1531
                     /locus_tag="PL106_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33315.1"
                     /translation="TVRLTARAAVACGQVHHIKIAVGDAGDTAYDSAVFLEGGSFTSA
                     PFVPTLQPGPGIVGLNTIMESCFNVEYIFSRVGDSTFAATVQVEIGGTATPGVDYSPA
                     FPPQITFAPFETQQTWTINAPLDQDGLETLDITLISQSVCASDSLEVTFHFFIDQPDP
                     LIAIGDAFLIACGESVQLTPTVTGGYGAYSYAWQPTGETDPTILYTPSTVSDVYITVS
                     DTCGLQTTGFFAVELTPLPPITATLTGPDPLIEGCDGATLTVTRPAGTTGDLAMNVWH
                     HGTATGGQDYSVPDPFIVPDGQPSGSSTVSPVEDNTPEGDETMIIEVGYTNACQQVAS
                     DTVQATIQDSPPLVLSGDSLLIIPCGGDSIPLDVEAVGGIAPLSYVWSNGHTGPTGYA
                     SNAVDGIYTVTATDDCGHTTSMDVIVDPQCAIIIPNVISPNGDGHNDVFYIDGILASK
                     STVRIFNRWGQVVYEASNYQNNWAPKDLPDGTYFYEVKVDREPDAFTGSLTILSSNRR
                     R"
     gene            complement(1542..>2006)
                     /locus_tag="PL106_09355"
     CDS             complement(1542..>2006)
                     /locus_tag="PL106_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755709.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNA33316.1"
                     /translation="MIPSPKVATYDLQPEMSAVGVADAIINGIAKDEARFICLNFANP
                     DMVGHTGVFPAIVKAVETVDACARRVVEAGRDRGWSFVIIADHGNADKAVNPDGSPNT
                     AHTTNPVPIIVLDDRDRLLRDGVLADVAPTLLELMGIEKPVEMTGSSLLVAG"
BASE COUNT          387 a          657 c          595 g          367 t
ORIGIN      
        1 gacggtacgc ctcaccgccc gtgccgcggt cgcctgcgga caggtccacc acatcaagat
       61 cgccgtgggc gatgctgggg atacggcgta cgacagcgca gtgttcctgg agggaggcag
      121 cttcaccagc gcgcccttcg tgcccaccct gcaaccaggc cccgggatcg ttggcctgaa
      181 caccatcatg gagagctgct tcaacgtgga atacattttc tcccgcgtgg gcgattccac
      241 tttcgcggcc acggtacagg tggagatcgg tggcaccgcc acccccggtg tcgattacag
      301 cccggctttc ccgccacaga tcacattcgc acccttcgaa acccagcaaa cctggaccat
      361 caatgctccg ctcgatcagg acggcctgga gaccttggat atcaccctga tctcacagtc
      421 cgtctgtgca tccgattcct tggaggtcac cttccacttc ttcattgatc aaccggaccc
      481 tttgatcgcc atcggggatg cgttcctgat cgcctgtggc gagagcgtcc agctcacccc
      541 caccgtaacg ggtggatacg gggcatacag ttacgcatgg caaccgaccg gcgaaacgga
      601 tcccaccatc ctgtacacgc ccagcacggt gagtgatgtg tacattacgg tatcagatac
      661 ctgcggcttg cagaccaccg gcttcttcgc ggtggaactg acgccactgc cgcccatcac
      721 ggccaccctt acgggccccg accccctgat cgaaggttgc gatggtgcca cgctcacggt
      781 gacgcgcccg gccggtacaa ccggcgacct ggcgatgaac gtgtggcacc atggcaccgc
      841 caccggtggg caggactatt ccgttcccga tccgttcatc gttcccgatg gccagccatc
      901 gggcagttcc acggtctccc cggtggagga caacaccccg gagggcgatg agacgatgat
      961 catcgaagtg ggctacacca atgcctgtca gcaggtggcc agcgacacgg tgcaagcgac
     1021 catccaggac agcccgccgc tggtgctctc gggcgacagt ctgctcatca tcccatgcgg
     1081 gggcgacagc attccgctgg acgtggaggc cgttggtggt atcgcgccgc tgagttacgt
     1141 atggagcaat gggcacacgg gtccgacggg gtacgcctcc aatgctgtgg acggtatcta
     1201 tacggtgaca gccaccgatg attgcgggca caccacctcc atggacgtca tcgtggatcc
     1261 gcagtgcgcc atcatcattc cgaacgtgat cagccccaat ggggatggcc ataacgatgt
     1321 gttctacatt gatggcatcc ttgcctcgaa gagcaccgtc cggatattca accgctgggg
     1381 gcaggtggtg tacgaggcct ccaactacca gaacaactgg gctccgaagg accttcccga
     1441 tggcacctat ttctacgagg tgaaggtgga ccgcgaaccg gacgcgttca ccggcagcct
     1501 caccatcctg agtagcaacc gccgccggta acgagcgggc ctcagcccgc gacaagcaat
     1561 gaagagcccg tcatttcgac gggcttttca atgcccatca gttccaggag ggtcggggcc
     1621 acatcggcca gcacgccatc gcgcaacagc cgatcccggt catcgagcac gatgatcggg
     1681 actggattgg tagtatgggc ggtgttgggc gaaccgtccg ggttcaccgc cttgtcggcg
     1741 ttgccgtgat cagcgatgat gacgaagctc cagccccggt cgcgacccgc ttccaccacg
     1801 cggcgggcac aggcgtccac cgtttccacc gccttcacga tggccgggaa cacgcccgtg
     1861 tggcccacca tgtcggggtt ggcgaagttc aggcagatga aacgggcctc atccttggcg
     1921 atcccgttga tgatggcgtc ggccaccccc acagcgctca tctccggctg aaggtcatag
     1981 gtggccacct tgggggaggg gatcat
//
LOCUS       DAOQOC010000564         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37939_length_2001_cov_2.657246, whole genome shotgun sequence.
ACCESSION   DAOQOC010000564 DAOQOC010000000
VERSION     DAOQOC010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37939_length_2001_cov_2.657246"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PL106_09360"
     CDS             complement(<1..652)
                     /locus_tag="PL106_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNA33317.1"
                     /translation="MRLLIGEVIERDDHPVIKKQTLVRVPIRLGEDVFSSGRISNARR
                     DHLIKSLKAFRLLTEVYDIPYVRCCATSAMREATNREETVARAEMESGVHIETIDGEV
                     EADLICNTFWTQDLLKDRNYLYIDVGGGSTELTWLEHGRRVQRDSFRIGTVRSLQSGI
                     DPQEQRRLHTFLDALRAEHGRLVAIGTGGNINRIFKENGNRFGEPLTRKAIRNQRDR"
     gene            952..>2001
                     /locus_tag="PL106_09365"
     CDS             952..>2001
                     /locus_tag="PL106_09365"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNA33318.1"
                     /translation="MRTALLLPSILIAAHVAAQTSVTVPTGAGNVEQTWYSLSTDAES
                     SAALAEWDLAFEINGGFNAGILANTAKGVKVYQTPYAVGDWGSMDTNGMAANWTGLFN
                     SDADWSQGAFNADVDLDIYNIGWGIYNTVTHVVAGDSIQVVVLTDGSARMIRIDALSA
                     GIYTFTYANLDGTDEQTHQLAKSDYTGKNFAYWSLTSHSAIDREPMSADWDILFGKYT
                     ANVGMWYGVTGALLNKGVEAVRVDNVPTNEAEYDWNGLDTVINTIGYDWKYFDMDNFI
                     YVIEDSLTYFVKDVPGNLWKIWFTGFGGSASGEITFNKELVSAVGIDGTTTASDMVLY
                     PNPTADDQVRVILDGF"
BASE COUNT          393 a          586 c          632 g          390 t
ORIGIN      
        1 tacgatcccg ttggttgcgt atggccttgc gggtgagcgg ttcgccgaag cggttgccgt
       61 tctccttgaa gatgcggttg atgttgccgc ccgttccgat cgccaccagc cggccgtgct
      121 cggcgcgcag tgcatccaga aaggtgtgca gtcgccgttg ttcctgcgga tcgatgccgc
      181 tctgtaatga tcgtacggtg ccgatccgga agctgtcgcg ttgcacacgg cggccgtgct
      241 ccagccaagt gagttcggtg ctgcccccgc ccacgtcgat gtacaggtag ttgcggtcct
      301 tcagcaggtc ctgcgtccag aaggtgttgc agatcagatc ggcctccacc tcgccgtcga
      361 tcgtttcgat gtggacaccg ctctccatct cggcgcgcgc gacggtctcc tcgcggttgg
      421 tggcctcacg catggcgctg gtggcgcagc agcgcacgta cgggatgtcg tacacctcag
      481 tgagcagacg gaaggccttg agactcttga tgaggtggtc gcgccgcgcg ttgctgatac
      541 gaccggatga aaaaacatcc tcacccaagc ggatcggcac gcgtaccagc gtctgcttct
      601 tgatgacggg atggtcgtcg cgttcgatca cctcgccgat gagcaggcgc acggcgttcg
      661 acccgatgtc gatggcggcg tatttcaggt cgtgagcgat gcgccgaagg taacctttga
      721 cgggctgcct ccgggatccg tcaatggagc gtcaagggat cgtcacggga tcgccataat
      781 ccatgcggac ggttcgcgga ttcgtgctcg cagaggactt tcgcggccat gaatggatca
      841 tcattctgcc cgaccaacga taattacccc gatcggggac ttgttgcccg gaacacgtcg
      901 tcgtcgtttt gcgccgccat gaaaagaacc gccacccttc tcgccgccga aatgagaaca
      961 gcactcctcc tcccttcgat ccttattgca gcgcatgtcg cagcgcaaac gtccgtaacg
     1021 gtccccaccg gagcgggcaa cgtggaacag acctggtaca gcctgagcac cgatgcggag
     1081 tcgagtgcgg cgctggccga gtgggatctc gccttcgaga tcaacggtgg attcaacgcc
     1141 gggatcctgg ccaacacggc gaaaggggtg aaggtgtatc agacgccgta tgccgtgggc
     1201 gattggggca gcatggacac caacggtatg gccgccaatt ggacgggcct cttcaacagc
     1261 gatgccgatt ggagccaggg ggcgttcaat gccgatgtgg acctggacat ctacaacatc
     1321 ggatggggca tctacaacac cgtgacgcat gtggtggccg gggacagcat ccaggtggtg
     1381 gtactcaccg atggaagcgc ccggatgatc cgcatcgatg cgttgagcgc agggatctac
     1441 accttcacct acgccaacct cgacggcacc gacgagcaga cgcaccagtt ggcgaagtcc
     1501 gactacaccg gaaagaactt cgcctactgg tcactcacga gccacagcgc catcgatcgt
     1561 gaaccgatga gtgccgactg ggacatcctg ttcggcaagt acacggccaa cgtgggcatg
     1621 tggtacggcg taacgggtgc gctgctgaac aagggcgtgg aggccgttcg cgtggacaac
     1681 gtccccacca acgaagcgga gtacgactgg aacgggctgg acaccgtgat caataccatc
     1741 gggtatgatt ggaagtactt cgatatggac aatttcattt acgtgatcga ggactcgctc
     1801 acctacttcg tgaaggacgt gccgggcaac ctgtggaaga tctggttcac gggcttcggt
     1861 ggcagcgcat cgggcgagat caccttcaac aaggagttgg tcagtgcggt gggcatcgat
     1921 gggacgacca cggcgagcga catggtgctg tatccgaacc ccaccgccga tgatcaggtg
     1981 cgcgtgatcc tcgacggctt c
//
LOCUS       DAOQOC010000565         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37962_length_2000_cov_3.680206, whole genome shotgun sequence.
ACCESSION   DAOQOC010000565 DAOQOC010000000
VERSION     DAOQOC010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37962_length_2000_cov_3.680206"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2000)
                     /locus_tag="PL106_09370"
     CDS             complement(<1..>2000)
                     /locus_tag="PL106_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33319.1"
                     /translation="QPTASDVYVITGTENISGCTQQYFFSVELGDPPLTDIIAPSSVC
                     AWTAVQLDLQLNPNGSYLYQWSPAAGLDNPHAQDPVATPAHDTWYHVDITTVTGCAAT
                     SDSVLVTVTDGNVLSVEASSEPALICEGGSAQLDVAVRRIIAEDDFDAGIGTPWEEVL
                     GGGANTVCGAVAGQALRFNDAGTRVARTVPLDVSAGGEIRFALKLCTGTAPCEDVDAG
                     ENIYVEYSLNGSSWTIMATYTEAMFPDFTAITLAIPLAAQTPTTRFRWRQLIHSGVDQ
                     DNWVLDDVAIAVDDPGGMSFTWTPPATLNDPAIHDPVAVPPASLSYTVSMTDPGTGCV
                     YQDSVMVLVGPMFELETTNDTAVCAPGLDVLLASVPQDPGNYTWVWSPDDGSLLSPLT
                     QSTTAMPLENTTYYVAVTSQFGCTVTDSVVVLVADALTVDAAAVPVTICQGQEVQLQA
                     QTWGGSGSIGFHWAPDATLDDPNSPAPVGTPLTSTTFTVTATDQLCGLIDIATVFVEV
                     LEAPPLDLGPDQPICPDETVVLDAGNADSWLWSTTATTQAITTDAPGTYWVDASIGAC
                     VAMDTIVLLDTPDPGELDFTVFGCENRSAVLTIPNGDGSYLWATGDTTRSITVETIGD
                     YPYTLVDTFGCTYAGLATLVVDPLQGGITVPNVISPNGDGKN"
BASE COUNT          324 a          616 c          690 g          370 t
ORIGIN      
        1 tcgttcttgc cgtcgccatt ggggctgatg acgttgggca cggtgatgcc gccctgcagc
       61 gggtccacca ccaacgtggc caggcccgcg taggtacatc cgaaggtgtc caccagggtg
      121 tacgggtagt cgccgatggt ctcaacggta atgctccgcg tggtgtcgcc cgtggcccag
      181 aggtagctgc catcgccgtt cgggatggtg agtacggcgc tgcggttctc gcaaccgaag
      241 acggtgaagt ccagctcgcc gggatcgggt gtatccagta gcacgatggt gtccatggcc
      301 acacacgcac cgatcgaggc atccacccaa taggtgcctg gcgcatccgt cgtgatcgcc
      361 tgtgtggtcg cggtggtgct ccaaagccag ctgtccgcgt tccccgcatc cagcaccacg
      421 gtctcatcag gacagatggg ctggtcggga cccaggtcaa gcggtggcgc ttccaatact
      481 tctacaaata cagtagcgat gtcaatgagc ccgcagagtt gatcggtggc ggtgacggtg
      541 aaggtggtgc tggtgagcgg agtgcccact ggcgcggggc tgttggggtc gtccaaggtg
      601 gcgtcgggcg cccaatggaa gccgatgctg ccgctgccgc cccaggtctg cgcttgcagt
      661 tgcacctcct ggccctggca tatcgttacg ggcacggcag cggcatccac ggtgagcgcg
      721 tcggccacca ggaccaccac gctgtcggtc accgtgcagc cgaactggct cgttaccgcc
      781 acgtaatagg tggtgttctc cagcggcatg gccgtggtac tctgggtgag cggtgagagc
      841 agcgatccgt catcggggct ccagacccac gtgtagttgc cggggtcctg gggcacggat
      901 gccagcagca cgtccaggcc cggggcgcag accgccgtgt cgttcgtggt ctccaattcg
      961 aacatggggc ccaccagtac catcaccgaa tcctggtaca cacagccggt gcccggatcc
     1021 gtcatggaga cggtgtacga gagcgaggcc ggtggcaccg cgacgggatc atggatcgcc
     1081 ggatcgttca aggtggcggg cggggtccag gtgaagctca tgccgccggg gtcgtccacc
     1141 gcgatggcca cgtcgtccag cacccagttg tcctgatcca cgccgctgtg gatcaattgc
     1201 cgccagcgga agcgggtggt gggggtctgg gcggccaacg gtatcgcgag cgttatcgcg
     1261 gtgaagtcgg ggaacatggc ctcggtgtac gtggccatga tggtccagct gctcccgttg
     1321 agcgagtatt ccacgtagat gttctcgccg gcgtccacat cctcgcacgg ggcggtgccg
     1381 gtgcagagtt tcagcgcgaa acggatctcg ccgcccgcac tcacgtccag gggcacggta
     1441 cgggccaccc gggtgcccgc atcgttgaag cggagcgcct gtccggcgac cgcaccgcat
     1501 acggtgttcg cacccccgcc gagcacctct tcccatggtg ttccgatgcc cgcatcaaag
     1561 tcatcctcgg cgatgatgcg ccgcacggcc acgtcgagct gggcgctgcc tccctcgcag
     1621 atgagggccg gctcgctgct ggcctccacg ctcagcacat tgccgtcggt caccgtcacc
     1681 agcacgctgt ccgaagtggc ggcgcagccg gtgaccgtgg tgatgtccac gtggtaccag
     1741 gtgtcgtgcg cgggcgtggc cacggggtcc tgcgcatggg ggttgtccag tccggcagcc
     1801 ggtgaccatt ggtacaggta ggaaccgttg gggttgagtt gcagatcgag ttgcacggct
     1861 gtccaggcgc acacgctgct gggggcgatg atgtcggtga ggggcggatc gcccagttca
     1921 acactgaaaa aatactgttg cgtgcaaccg ctgatgttct cggtaccggt gatcacgtac
     1981 acatcgctgg cggtgggctg
//
LOCUS       DAOQOC010000566         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37974_length_2000_cov_2.978920, whole genome shotgun sequence.
ACCESSION   DAOQOC010000566 DAOQOC010000000
VERSION     DAOQOC010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37974_length_2000_cov_2.978920"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /gene="hisS"
                     /locus_tag="PL106_09375"
     CDS             <1..1150
                     /gene="hisS"
                     /locus_tag="PL106_09375"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNA33320.1"
                     /translation="VKGKLMGGEEVHPGKLAAELSEKALRYDLTVPFARYVVMNQNEL
                     AFPFKRYQIQPVWRADRPQKGRYREFYQCDADMIGSKSLLNEVELIALFDEVFTALGL
                     SVVIKMNDRKVLSGIAEVSGQPEKVVDMVTAIDKLDKIGREKVEEEMRTKGISDEAIA
                     RIAPLFALNGSWNDQRPQLERWLSSSVVAQQGLKDLSFTFEAVGRLKCAKLEFDPTLA
                     RGLDYYTGAIFEVKSAEGTMPGSICGGGRYDDLTGVFGLKGMSGVGISFGADRIYDVL
                     LETNKFPADLGASTKLLFANFGEAEAKHCLGLLRQAREAGIAAELYPDAVKLPKQFTY
                     AEKRSIPYVAIVGGTELKENTVTLKRMGAGEKGESLSMEQVIERINNG"
     gene            1147..>2000
                     /locus_tag="PL106_09380"
     CDS             1147..>2000
                     /locus_tag="PL106_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNA33321.1"
                     /translation="MKPHLIGTVGLELAELAHILTTRSPIALDPDAVAAVRRSHAYLR
                     DRLKNSDAPIYGVNTGFGSLADTRIDKKDLAQLQKNLVMSHACGSGDPVPELIVRAML
                     VLKVQNMAFGHSAVAPATVQRLIDMYNADMLPVVYERGSLGASGDLAPLAHLSLPLLG
                     LGEVIVAGKRMSAGQALKKFGWAPLELGPKEGLALLNGTQFMNAYAALLVLKATRLAD
                     LADRIAAMSLDAYDGRTEPFHASVHAVRPHPGQAVVAGHLRELLQGSGIATRAKKHVQ
                     DPYSFRCIP"
BASE COUNT          408 a          580 c          666 g          346 t
ORIGIN      
        1 agtgaagggg aagctcatgg ggggagagga agtgcatccc ggtaagctgg cggctgaact
       61 gagtgagaaa gccctgcggt acgacctcac ggtccctttc gcccgctacg tggtgatgaa
      121 ccagaacgaa ctggcctttc ccttcaagcg gtatcagatc cagcctgttt ggcgcgcgga
      181 caggccgcag aagggacgtt atcgggaatt ctaccagtgc gacgcggaca tgatcggcag
      241 caagagcctg ctgaacgaag tggaactcat cgccctgttc gatgaggttt tcaccgcatt
      301 ggggctttcg gtagtgatca agatgaacga ccggaaggtg ctcagtggca tcgccgaggt
      361 gagcggccag cccgagaagg tcgtggacat ggtgaccgcg atcgacaagc tggacaagat
      421 cggtcgcgag aaagtggagg aggagatgcg cacaaaaggc atttcggatg aagccatcgc
      481 acgcatcgct ccgctgttcg ccttgaacgg ttcgtggaac gatcagcgac cccagcttga
      541 acggtggctc tcttcctctg tggtcgcgca gcagggcctg aaggatctct cattcacctt
      601 cgaggcggtc ggacggctga agtgcgccaa gctcgaattc gatcccacgc tcgcccgtgg
      661 actggactat tacaccgggg ccatcttcga ggtgaagagc gccgaaggca ccatgcccgg
      721 cagcatttgt ggtggtggac ggtacgacga cctcaccggt gtcttcggcc tgaagggcat
      781 gagcggtgtg ggcatcagct tcggcgcgga ccgcatctac gacgtattgc tggagacgaa
      841 caaattcccg gcggaccttg gtgcgagcac gaaactgctc ttcgccaact tcggcgaggc
      901 cgaggccaag cactgtctgg ggcttctgcg gcaggccaga gaggcgggca ttgcggcaga
      961 attgtacccg gacgcggtga agctacccaa gcagttcacc tacgcggaga agagatccat
     1021 accatacgtg gccatcgtgg gcgggaccga actgaaggag aacaccgtta cgctgaagcg
     1081 gatgggggcg ggggagaaag gggagtcgct cagcatggaa caggtcatag aacgcatcaa
     1141 caacggatga aaccccattt gatcggcaca gtcggcctgg aactggcgga actggcgcat
     1201 atcctgacca cgcgcagtcc catcgccctc gatcccgatg cggtggcggc ggtgaggcgc
     1261 agccacgcat acctgcgcga ccggctgaag aacagcgacg cgccgatcta cggcgtgaac
     1321 acgggtttcg gctcgctggc cgacacgcgc atcgacaaga aggacctggc ccagttgcag
     1381 aagaacctgg tgatgagcca tgcctgcggc agcggcgatc ctgttccgga gttgatcgtg
     1441 cgcgccatgc tcgtgctgaa ggtgcagaac atggctttcg ggcacagcgc ggtggcgccg
     1501 gccacggtgc agcggctcat cgacatgtac aatgccgata tgctgccggt ggtgtacgaa
     1561 cgtggttcgc tcggagcctc cggtgatctg gccccgctcg cgcacctcag cctgccattg
     1621 ctcggcctcg gcgaagtgat cgttgcgggg aaacgcatgt ccgcaggtca agcgctcaag
     1681 aagttcggct gggcgcccct ggaactcggc ccgaaggagg gactggccct gctcaacgga
     1741 acgcagttca tgaacgccta tgcggcgttg ctcgtgctga aggccacgcg actcgcggat
     1801 ctcgccgatc ggatcgccgc catgtccttg gacgcgtacg atgggcgcac ggaacccttt
     1861 cacgcatcgg tgcatgcggt gcggccgcac cccggacagg ccgtggtggc gggccacctg
     1921 cgcgagttgc tgcaaggaag cggcatcgcc acccgggcga agaagcacgt gcaggatccc
     1981 tactcgttcc gctgtatccc
//
LOCUS       DAOQOC010000567         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_37985_length_2000_cov_2.433933, whole genome shotgun sequence.
ACCESSION   DAOQOC010000567 DAOQOC010000000
VERSION     DAOQOC010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37985_length_2000_cov_2.433933"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..436
                     /locus_tag="PL106_09385"
     CDS             74..436
                     /locus_tag="PL106_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33322.1"
                     /translation="MTRFVARAPAGACMVTPFTPTQGFYERLPFDDGVAQNIQNANGD
                     LGLLYGDLNSKRLPAYHRLDLGLTKTWKFSEHQMIQADVSVTNTYDRRNIFYFDRVTA
                     SRKDQLPILPSVGLSWTF"
     gene            638..>2000
                     /locus_tag="PL106_09390"
     CDS             638..>2000
                     /locus_tag="PL106_09390"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNA33323.1"
                     /translation="MTGSTKYIFVTGGVTSSLGKGIVSASLAKLLQARGFTVTIQKLD
                     PYINVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLDAATSQANNVTTGRIYQNVI
                     QKERKGEYLGKTVQVIPHITDEIKRNIQALGRKGIYDFVITEVGGTVGDIESLPYVES
                     MRQLKWEKGKDAINIHLTLLPYLSTTGELKTKPTQHSVKELLSLGIQPDVLVCRTEHP
                     MQKGMKEKIALFCNVEPRAVIESADAETIYDVPLLMQKEGLDQVVLDKLGITGYPEAD
                     LTQWTSFLHHYKEPKGEVNIGLVGKYVELKDAYKSIKEALDHAGADQELKVNIRWVHS
                     DKLTPKNLAKQLGGLSGILVAPGFGHRGIEGKIEAVRYARENRIPFLGICLGMQCAVI
                     EFARNVLEFNAANSTEMDAATPYPVIAMMEEQKVIENKGGTMRLGAYPCKLSDGSKVR
                     AVYG"
BASE COUNT          457 a          592 c          612 g          339 t
ORIGIN      
        1 aaaattgaca tgataagggt gttgtgtgtg gtggcccgat gagggcgccc ccagactttc
       61 cgtacccata agcgtgaccc ggttcgttgc ccgtgctcca gcgggtgcgt gcatggtcac
      121 cccgttcacc cccacccaag gcttctacga gcgcctgccc ttcgacgatg gcgtggcgca
      181 gaacatccag aacgccaacg gcgatctggg actgctgtac ggcgacctca acagcaagcg
      241 cctgcccgcc taccatcggt tggacctggg gctgaccaag acgtggaagt tcagcgagca
      301 ccagatgatc caggcggatg tgagcgtaac gaacacatac gaccgccgga acatcttcta
      361 ctttgaccgg gtgaccgcca gccgcaagga ccaattgccg atcctgccca gcgtagggtt
      421 gagttggacg ttttgagggc attgccgtag gtactggagc gagcagccga cagcaggcag
      481 gtcgcagcgg acagcaggcg gcccatccat gttgttccgc ggccccctgc agctcgctgc
      541 ctggtaccgg gtcaaccaaa aacccccttc cgcttcgcgt atccgttatc tttgtgcccc
      601 gtgatcgcgg ctcaattcag agccctctca ccaagcgatg acgggttcca ccaagtacat
      661 cttcgttacg ggcggcgtta ccagcagctt gggcaagggc atcgtgagcg cttcgctcgc
      721 caagctgctg caagcacgcg ggttcaccgt gaccatccaa aagctggacc cgtacatcaa
      781 tgtggacccc ggcacgctga acccgtatga gcatggggag tgcttcgtaa cggaggacgg
      841 ggcggagacc gacctggacc tgggccacta cgaacgcttc ctggatgcgg ccacctccca
      901 ggcgaacaac gtcaccaccg ggcgtatcta ccagaacgtc atccagaagg agcgcaaagg
      961 cgaatacctg ggcaagaccg ttcaggtgat cccccatatc accgacgaga tcaagcgcaa
     1021 catccaggcg ctggggcgca agggcatcta cgactttgtg atcaccgaag tgggcggtac
     1081 ggtaggcgac atcgagagcc tgccctatgt ggaatccatg cgccagttga agtgggagaa
     1141 gggaaaggac gccatcaaca tccacctcac actcctgccc tacctgagca ccacgggcga
     1201 attgaagacc aaacccacac agcacagcgt gaaggaactg ctcagcttgg ggatccagcc
     1261 cgatgtactg gtgtgccgca ccgaacatcc catgcagaag ggtatgaagg agaaaatcgc
     1321 cttgttctgc aacgtggagc ctcgtgctgt gatcgagagt gcggacgcgg agaccatcta
     1381 cgacgtaccc ctgttgatgc agaaggaagg tctggaccag gtggtgctgg acaaactcgg
     1441 catcaccggt tacccggagg ccgacctcac gcaatggacg agcttcctgc atcactacaa
     1501 ggagccgaag ggagaggtga acatcggcct agtgggcaag tacgtggagt tgaaggacgc
     1561 ctacaagagc atcaaggaag cgttggacca cgccggggcg gaccaggaat tgaaggtgaa
     1621 catccgctgg gtccacagcg acaaactcac tccgaagaac ctggccaaac aattgggcgg
     1681 gctcagtggc atcctggtgg caccgggctt cggccaccgg ggcatcgagg gcaagatcga
     1741 ggcggtgcgt tatgcccgcg agaaccgtat ccccttcctg ggcatctgcc tcggcatgca
     1801 gtgcgcggtg atcgaattcg cccgcaacgt gctggaattc aacgcggcga acagtaccga
     1861 gatggatgcg gccaccccgt acccggtgat tgccatgatg gaagagcaaa aggtgatcga
     1921 gaacaagggc ggtaccatgc gtttgggagc atacccgtgc aaactcagcg atggcagcaa
     1981 ggtgcgggcg gtgtacggca
//
LOCUS       DAOQOC010000568         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38050_length_1998_cov_3.329902, whole genome shotgun sequence.
ACCESSION   DAOQOC010000568 DAOQOC010000000
VERSION     DAOQOC010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38050_length_1998_cov_3.329902"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="folP"
                     /locus_tag="PL106_09395"
     CDS             complement(<1..823)
                     /gene="folP"
                     /locus_tag="PL106_09395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNA33324.1"
                     /translation="MHTASTHTWRLRDRLFTPHFPLVMGIINVTPDSFHAQGRIDSVD
                     DALHMAERMLAQGAAILDIGGQSSRPGSGPGDEREERRRVIPVIEAVHRAFPDALISV
                     DTWRAAVAAEAVNFGARLVNDISAGLLDAAMLPTVAALGVPYVAMHMQGTPATMQQAP
                     VYNDVLAEVTLFLSQRLHAARAAGIADVIIDPGFGFGKTAAHNFALLHGLARLQALDA
                     PVLAGLSRKRMINSVLGTTPADALNGTTVLNTVALLNGAAILRVHDVKEAVECVKL"
     gene            945..>1998
                     /locus_tag="PL106_09400"
     CDS             945..>1998
                     /locus_tag="PL106_09400"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33325.1"
                     /translation="MAITGASSQNTDSLWQVWSNPGLPDTVRYHALGALSWANMFTRT
                     DSALSLALLMYERSGEKQDRRVQAQALTYQGIAYNVLGNHFAARECYAQMLELFQALG
                     DERGVAGAYNNLGALYHEQGDHLAAISWYGKSIAIFEELRDDKGRAAAYNNISGIYNA
                     LGDSARAFEYANKCLAMHQARGDRAAAASILGNIGLIHLNNGRPDAALRILNESARQK
                     ALIGDPASEAKTFLDIGEAYRQKGEPDSATHWLNQALAIQDSLKNESGQASTCVAIGQ
                     VHLDRSDPQAAQTSCERALAIARDLHLRSVERRACDCLYRAAKDLGDDRSALHYFERA
                     AALTDSLHAEEVRKGLE"
BASE COUNT          375 a          617 c          666 g          340 t
ORIGIN      
        1 cgagcttcac gcactccacg gcctccttca cgtcatgaac ccgcaggatg gccgcaccgt
       61 tcagcagggc gacggtgttg agcacggtcg tgccgttgag cgcatcggcc ggggtggtcc
      121 cgagcaccga attgatcata cgcttgcgcg aaaggcccgc gagtaccggc gcatccagtg
      181 cctgcaggcg ggccaggccg tgcaacagtg cgaagttgtg cgcagcggtc ttgccgaagc
      241 cgaagccggg atcgatgatc acatcggcga tgcccgccgc tcgtgccgcg tgaagccgct
      301 ggctcaggaa gagcgttact tccgccagca catcgttgta cacgggagcc tgttgcatgg
      361 tggctggtgt gccttgcatg tgcatggcca cgtagggcac gccgagcgcg gccactgtgg
      421 gcagcatggc cgcatccagc agcccggcac tgatgtcgtt caccaggcgg gcgccgaagt
      481 tcacggcctc cgccgctacg gccgcccgcc acgtgtccac gctgatgagc gcatccggaa
      541 aggcgcgatg caccgcttcg atcaccggga tgacgcgtcg tcgttcctcg cgttcatcac
      601 cagggccact gcccgggcgc gaactctgtc ctccgatgtc caggatggcg gcaccctgcg
      661 ccagcatgcg ctcggccatg tgcaaagcgt cgtccaccga gtcgatgcgt ccctgcgcgt
      721 ggaacgaatc cggcgttacg ttgatgatgc ccatcaccaa ggggaagtgc ggcgtgaaaa
      781 gccgatcgcg caggcgccag gtgtgggtgg aggcggtgtg catgggtgcg caagttgcga
      841 acacgcacgc atgcgaccat ccgagaacag ctttcttctt cgcaacttgc ggcatgttcg
      901 gaagtcgtag cgctgggcgc cttttcgcct ccctggcttg ttccatggcg atcacagggg
      961 cctcctcaca gaatacggac agcctttggc aggtgtggag caacccgggc ctgccggata
     1021 cggtgcgcta ccacgcattg ggtgccctgt catgggccaa catgttcacc cggaccgaca
     1081 gcgcactgtc cctggccctg ctgatgtacg aacggagtgg tgagaagcag gaccgcaggg
     1141 tgcaggccca ggccctcacg taccagggca ttgcgtacaa cgtgctgggg aatcactttg
     1201 ccgcgcgcga gtgctatgcg cagatgcttg aactgttcca ggcgctcggg gatgagcgcg
     1261 gggtggccgg ggcgtacaac aacctgggtg cgctgtacca cgaacaaggc gaccacctgg
     1321 ccgcgatcag ctggtacggc aagagcatcg cgatcttcga ggaactccgt gacgacaagg
     1381 gtcgtgctgc agcgtacaac aacatcagcg ggatctacaa cgcgttgggc gatagtgcgc
     1441 gtgcgttcga gtacgcgaac aaatgcctcg ccatgcacca ggcacgtggc gaccgggccg
     1501 cggctgcgag catcctcggg aacatcggac tcatccacct gaacaatggc cggccggatg
     1561 ctgcgctgcg gatcctgaac gagagtgcgc ggcagaaggc cctgatcggc gatccggcga
     1621 gcgaggcgaa gacctttctg gacatcggcg aggcgtatcg ccagaaaggg gaaccggaca
     1681 gcgcgaccca ttggctgaac caggcgctgg cgatccagga cagcttgaag aatgaaagcg
     1741 ggcaggcctc cacgtgtgtg gccatcgggc aggtacacct ggaccgttcc gatccgcaag
     1801 cagcgcaaac atcgtgcgaa cgggcgttgg ccattgctcg cgacctgcat ttgcgctccg
     1861 tggagcgccg tgcgtgcgac tgtctgtaca gagcggccaa agatctgggt gatgatcggt
     1921 ccgcattgca ctatttcgaa cgggccgctg cactcaccga tagcctgcac gccgaggagg
     1981 ttcggaaggg cttggagc
//
LOCUS       DAOQOC010000569         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38118_length_1996_cov_3.561051, whole genome shotgun sequence.
ACCESSION   DAOQOC010000569 DAOQOC010000000
VERSION     DAOQOC010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38118_length_1996_cov_3.561051"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..713)
                     /locus_tag="PL106_09405"
     CDS             complement(45..713)
                     /locus_tag="PL106_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNA33326.1"
                     /translation="MDLREIAALITLEWRLDRRQKHAWGSLALYVVSTVYTCYLGVKR
                     LSAVPTWNALFWIILLFAAFNALSRSFQREEGGRQLYLHTLVHPRSVVLARTLYNAAT
                     MVVLSLISLVTYVLFIGDRALEAADPGLFITTVLLGAVGLACTLTLIAAIAARAGSGP
                     GLMAILGFPIVLPLLLAILRASKLALDGVAWSVTGNYLLAILAMDVLAVALSAILFPY
                     LWRD"
     gene            complement(789..1613)
                     /locus_tag="PL106_09410"
     CDS             complement(789..1613)
                     /locus_tag="PL106_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33327.1"
                     /translation="MRTPISFAPLLLFLAAAAQPQQVKITAPAVLDAPGAVHRNKPAQ
                     AALMASGITAEQMDQVISYGDREHWPAGIKVDSLRAKNQPYIQNYVCFRVAGFTEDSL
                     PKTVIMVPARENIHMPEVMRPLSDLYVIVPDSSITNVQPGKARPEISRGPKWKNLPPA
                     RILVPEELYATYDLGTDSAALKALQEKGMSQPEIDAVVFRSIERNWPDGIDSFEERQA
                     LLPKFKKYKAYVGAKWDDKVLLIIPVEKNRKQPVLMRPFVDLYLVYNKSAVDVKKK"
     gene            1739..>1996
                     /locus_tag="PL106_09415"
     CDS             1739..>1996
                     /locus_tag="PL106_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNA33328.1"
                     /translation="MKQPILNLVSLAAMVFGCGRGTVYTQPPVQPFHALSATDINGAP
                     FSFAQLKGHKVMVVNTASECGFTPQYEKLEQLYTKYKDKGFI"
BASE COUNT          388 a          617 c          606 g          385 t
ORIGIN      
        1 cagcaacagg agcgttgcca gcagcttcca ccagaggccc ttcatcagtc gcgccaaagg
       61 taggggaaga ggatcgcgga caaggcgacg gccagcacgt ccatcgcgag tatggccagc
      121 aggtagttgc cggtgaccga ccaggccacg ccatcgagcg ccagtttgct cgcgcgcagg
      181 atcgccaaca gcaagggcag cacgatgggg aagcccagga tcgccatcag gccgggacca
      241 ctgcccgcgc gagccgctat ggccgcgatc aacgtgagtg tacatgcaag gccgaccgca
      301 cccagtaaca cggtggtgat gaacaggccc gggtccgcag cttccaacgc ccggtccccg
      361 atgaagagca cgtacgtcac caagctgatg aggctgagca cgaccatggt cgccgcgttg
      421 tacagggtgc gcgccagcac cacgctgcgt gggtggacca acgtgtggag gtagagttgc
      481 cgtccgccct cctcgcgctg gaacgatcgg gacaacgcgt tgaaagcagc gaagagcagg
      541 atgatccaga acagcgcgtt ccacgtgggc acggcgctca gccgtttcac cccaaggtag
      601 caggtgtaca cggtgctcac cacgtacagc gccaggctgc cccaggcgtg tttctgacgg
      661 cgatcaagcc gccattcaag cgtgatcaat gcggcgatct cccgtaggtc cacggggcga
      721 agctatccgg acttatagaa gggcaccatc ttgcgacggt ggaagtggac atcgcgcccg
      781 gcccggtgct atttcttctt cacatccacc gcgctcttgt tgtacaccag gtacaagtcc
      841 acgaagggac gcatgagtac cggctgtttg cggttcttct ccacgggaat gatcagcagg
      901 accttgtcgt cccacttggc gcccacataa gccttgtatt tcttgaattt tggcagaagg
      961 gcttgccgct cctcgaaact gtcgatccca tcgggccagt tgcgctcgat gctccggaaa
     1021 acgaccgcgt cgatctcggg ctggctcata cctttctcct gcagcgcctt cagtgcggca
     1081 ctgtcggtgc ccagatcata cgtggcgtag agttcctcgg gcaccaggat ccgcgctggg
     1141 ggcaggtttt tccacttggg cccacgactt atttccggtc gggctttgcc gggctgcacg
     1201 ttcgtgatcg aactgtcggg aacgatcacg tacaggtcgc tcaacggccg catcacttcg
     1261 ggcatgtgga tgttttcccg ggccggtacc atgatcacgg tcttcggcag gctgtcctcg
     1321 gtaaaacccg ccacgcggaa acacacgtag ttctggatgt agggctggtt cttggcgcga
     1381 agactgtcca ccttgatccc tgcgggccag tgttcccgat cgccgtaact gatcacctgg
     1441 tccatctgct cggctgtaat tccactggcc atcaacgcgg cttgggccgg tttgttcctg
     1501 tgtacggccc ccggggcatc cagcactgcg ggtgctgtga tcttcacctg ttggggctga
     1561 gccgcggcgg ccaggaacaa aaggaggggt gcgaaggaga tcggggtacg catggaaggt
     1621 gctcttacgc aagaaccgtg gcgaaaggta ccctccaacc tttgggctgc ggtcgcgccc
     1681 ccgtccaagg tactttcgcc gcgggcatgc catcggtggc ttgcgccttg tccaactgat
     1741 gaaacagccg atcctcaacc ttgtttccct ggccgccatg gtcttcggct gcggccgtgg
     1801 aaccgtgtac acccaaccgc ccgtgcagcc cttccacgcc ctgagcgcta ccgacatcaa
     1861 tggcgcaccg ttctccttcg cccaattgaa gggccataag gtgatggtgg tgaataccgc
     1921 cagcgaatgc ggtttcaccc cgcaatacga gaagctggag cagttgtaca cgaagtacaa
     1981 ggacaagggg ttcatc
//
LOCUS       DAOQOC010000570         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38120_length_1996_cov_3.206595, whole genome shotgun sequence.
ACCESSION   DAOQOC010000570 DAOQOC010000000
VERSION     DAOQOC010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38120_length_1996_cov_3.206595"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /gene="argS"
                     /locus_tag="PL106_09420"
     CDS             <1..1678
                     /gene="argS"
                     /locus_tag="PL106_09420"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNA33329.1"
                     /translation="FEGDITINVFPFLKLTGKGPEQVATAIGEHLVAEVPVVERFNVI
                     KGFLNIVIADRYWLDFLAEAATQDLLALAPTGKRVMVEYASPNTNKPLHLGHLRNIFL
                     GWSVSRILEASGNEVVKVQVINDRGIHICKSMLAWQRFGNGETPVSSGLKGDKLVGKY
                     YVEFDKAYRAQTDALVAEGKTKDEAEKQAPILLEAQDMLRKWEANDPEVRSLWATMNG
                     WVYDGFNATYARIGVGFDKNYYESQTYVLGKNDVLEGLKKGVFYEKDGAVWVDNTAEG
                     LDEKVLLRRDGTSVYMTQDIGTAIERFREYPDLRQLIYTVGNEQDYHFKTLFIILKKL
                     GFPWAGELFHLSYGMVDLPSGKMKSREGTVVDADDLIEEVVGEARRTGEQLSKLDEFN
                     DAERTALFEMIGQAALKYFLLKVDPRKRMLFDPAASIDLQGHTGPFIQYTYARIRSLL
                     RKADVAKGTSSFEPTGMEALLPEERTVLKHLHQFPGVLNDAAAKLDPAALANHTYETV
                     KAYNAFYQAVPVLKEMDPGKRAFRLAMSGAVAAATKKAMWCLGIEVPERM"
     gene            complement(1701..>1996)
                     /locus_tag="PL106_09425"
     CDS             complement(1701..>1996)
                     /locus_tag="PL106_09425"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA33330.1"
                     /translation="DQLDVGAYFDHEKLTVGLWYRGIPALKAYQPGYPNDESVIAMLG
                     YQTPQQIRFTYSYDITVSMLTMKSGGAHELSVMYEWPKRTRNRRWRAVPCPKF"
BASE COUNT          412 a          579 c          641 g          364 t
ORIGIN      
        1 gttcgagggc gacatcacga tcaatgtctt cccgttcttg aagctcaccg gcaaagggcc
       61 cgagcaggtg gccacggcca tcggtgagca cctggtggcc gaggtcccgg tggtcgagcg
      121 cttcaacgtg atcaagggct tcctcaatat cgtgatcgcg gatcgctact ggctcgattt
      181 tctggccgag gccgcaaccc aggacctcct cgccctcgca ccaacgggca agcgcgtgat
      241 ggtggagtat gccagcccca ataccaacaa gccgctgcac ctgggccatt tgcgcaacat
      301 cttcctgggc tggagcgtga gcaggatcct ggaggcctcc ggtaatgagg tggtgaaggt
      361 ccaagtgatc aatgatcgcg gcatccacat ctgcaagagc atgctggcct ggcagcgctt
      421 cgggaacggc gaaacacccg tcagcagcgg tctcaagggc gacaagctgg tggggaaata
      481 ctacgtggag ttcgataagg cgtaccgggc gcagacggac gccctggtgg ctgaaggcaa
      541 gaccaaggac gaggccgaga agcaggcgcc catcctgctc gaagcccagg acatgttgcg
      601 caagtgggag gccaacgatc ccgaggtgcg ttccctctgg gccaccatga acggctgggt
      661 ctacgacggg ttcaacgcaa cgtacgcacg catcggtgtg ggctttgaca agaactacta
      721 cgagagccag acctatgtcc tcggcaagaa cgacgtgctg gagggcctca aaaagggcgt
      781 cttctacgag aaggacggcg ccgtgtgggt ggacaacacg gccgaaggac tcgacgagaa
      841 ggtgttgctg cgccgcgatg gcaccagtgt gtacatgacg caggacatcg gtacggccat
      901 tgagcgtttc cgcgagtacc cggatctccg tcagctcatc tatacggtgg gcaacgagca
      961 ggactatcac ttcaagacgc tcttcatcat cctcaagaaa ctcggcttcc catgggccgg
     1021 cgagttgttc cacctgagct acggcatggt ggacctgccc agcggcaaga tgaagagccg
     1081 cgaaggcacc gtggtggatg cggacgacct gatcgaagag gtggtgggtg aggcgcgccg
     1141 caccggtgag cagttgagca agctggacga gttcaacgat gcggagcgga ccgccttgtt
     1201 cgagatgatc ggccaggccg cactgaagta tttcctgctg aaggtggatc cgcggaaacg
     1261 catgctcttc gatcccgcgg ccagcatcga cctgcaaggt cataccgggc cgttcatcca
     1321 gtacacctat gcgcggatcc gctccttgtt gcggaaggcg gatgttgcaa agggtacatc
     1381 gtcattcgag cccacgggca tggaggcatt gctcccggag gagcgtacgg tgctcaaaca
     1441 ccttcaccag ttccccggcg tgctcaacga tgcggccgcc aagctcgatc cggcggcgct
     1501 ggccaatcat acctatgaaa cggtgaaagc gtacaatgcc ttctatcagg ctgttcccgt
     1561 actgaaggag atggatccgg gcaaacgggc gttccgcctc gcgatgagcg gtgcggtggc
     1621 cgctgccacg aagaaggcca tgtggtgcct ggggatcgag gtgcccgagc ggatgtgacc
     1681 gggaggatcc ggaccggcta tcagaacttc gggcaaggca cggccctcca gcgccggttg
     1741 cgcgtgcgct tgggccattc gtacatcaca ctcaactcgt gtgcgccgcc actcttcatg
     1801 gtgagcatgc tcaccgtgat gtcgtagctg tacgtgaagc ggatctgttg cggggtctgg
     1861 taaccgagca tggcgataac cgactcgtcg ttcgggtatc cgggctggta tgccttcagg
     1921 gccggaatgc cccggtacca caggcctacg gtcagctttt catggtcgaa ataggccccc
     1981 acatccagct ggtccc
//
LOCUS       DAOQOC010000571         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38156_length_1995_cov_3.728351, whole genome shotgun sequence.
ACCESSION   DAOQOC010000571 DAOQOC010000000
VERSION     DAOQOC010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38156_length_1995_cov_3.728351"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>1995
                     /locus_tag="PL106_09430"
     CDS             10..>1995
                     /locus_tag="PL106_09430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNA33331.1"
                     /translation="MLPWRTLATAAIITSWCGLRAQVVLNEVCVTNMNGLEVTDPFNG
                     GQDREDWVELYNTGGSAVDLSGWYLSDNDALPTKWTFPAGATIPANGYLVVLCSGWNG
                     QYGGYWCTNFKLTQTSEEPIILSDAGGTPVDQYQLTLRTQMDHTRSRVPDGGTWSLTT
                     TSTMGAANAAATPEYTARPQLSPQAGFYSGAQQVSITGPAGTTIRYTTNGDEPTAAST
                     AYSGPINVSATTVIRAACFSDTPGPPRSFVETNTYFIGVTHGVAVLSMSGDEVQTLLD
                     GNGGIQPLGSLEYFGPDGQLRDEATGNFNEHGQDSWAYSQRGFDYIARDQTGYNDAMH
                     YPIFHATDRDKFQRLIIKALAGDNCPFGPGQPAHIRDPYVQSLSQLGNLKLDERSYEP
                     AVLYVNGQYWGVYDMREKVDDSDFFDEYYDQGENDLYCIKTWGGTWSEFGGGAAQADW
                     DALRAYIMGNNMGDATAFAYVDDRYNWKSLIDYFCLNSYVVCADWLNWNTMWWKGLDP
                     AGDHKKWGYCLWDEDATFGHYTNFTGIPDQSANADPCTVEELDDPGGQGHTTILNKLI
                     TENQMVHDYYVNRYIDLGNTLFSCATMNAHLDSLLALFTPEMPAQCARWGTTMAAWEA
                     GVQQLRDFINARCVTTQQGMVDCYDLEGPYNVTLVVQP"
BASE COUNT          429 a          629 c          605 g          332 t
ORIGIN      
        1 cccatgcaga tgctcccctg gcgaaccctt gctaccgcag cgatcatcac ttcctggtgc
       61 ggtctccggg cccaggtggt gttgaacgag gtgtgcgtca ccaacatgaa cgggctggag
      121 gtgaccgacc ccttcaatgg tgggcaggac cgcgaggatt gggtggagtt gtacaacacg
      181 ggcggctctg cggtggacct gagcggctgg tacctgagcg acaacgatgc gctgcccacc
      241 aaatggacct tcccggccgg ggcgaccata ccggcaaatg gatatctggt ggtgctctgc
      301 tccggatgga acggacaata cggcggctac tggtgcacca atttcaaact gacccagacc
      361 tccgaggagc ccatcatcct ctccgatgcg ggcggcacac cggtggacca gtatcaatta
      421 acactgcgca cgcagatgga ccatacgcgc agccgggtgc ccgatggcgg cacatggtcc
      481 ctcaccacca cgtccaccat gggtgcggcg aacgcggcgg ccacacccga gtacacggcg
      541 cggccccaat tatcaccgca agcgggtttt tattccgggg cgcaacaggt gagcatcaca
      601 ggtccggccg gcaccaccat ccgttacacc accaacggcg acgagcccac tgcggcctcc
      661 acggcgtaca gcggcccgat caacgtgtcg gccaccaccg tgatcagagc ggcgtgcttc
      721 agcgataccc cggggcctcc gcgcagtttc gttgaaacga acacctactt catcggggtg
      781 acgcatggag tggccgtgct gagcatgtcg ggcgatgagg tacagaccct gcttgacggc
      841 aatggcggga tccagccact gggcagcctg gaatacttcg gtcccgatgg tcaattgcgc
      901 gacgaggcca ccggcaactt caacgagcac ggacaggaca gctgggcata cagtcagcgc
      961 gggttcgact atatcgcacg tgaccagacg gggtacaacg atgcgatgca ctatcccatc
     1021 ttccacgcta ccgaccgcga caagttccag cgcctcatca tcaaagcatt ggctggcgac
     1081 aactgcccct tcggaccagg ccagcccgcc cacatccgtg atccatacgt gcaatcgctc
     1141 tcgcaactgg gcaacctcaa gctggacgaa cggagctacg agcccgccgt cctgtacgtg
     1201 aacggccagt actggggcgt gtacgacatg cgcgagaagg tggacgactc cgacttcttc
     1261 gacgaatact acgaccaggg cgagaacgac ctgtactgca tcaagacctg gggcggcacc
     1321 tggagcgagt tcggtggtgg tgccgcacag gccgattggg acgcgctgcg cgcctacatc
     1381 atgggcaaca acatgggcga tgccaccgca ttcgcgtacg tggacgaccg gtacaattgg
     1441 aaaagcctga tcgactactt ctgcctgaac agctacgtgg tgtgcgccga ctggctgaac
     1501 tggaacacca tgtggtggaa gggactggac cctgccggtg atcacaagaa gtggggctat
     1561 tgcttgtggg acgaggacgc caccttcggg cactacacca acttcaccgg catacccgac
     1621 cagagtgcca atgccgatcc ctgcacggtg gaggaactgg acgatcccgg tggacaaggg
     1681 cacaccacca tcctgaacaa gctgatcacc gagaaccaga tggtgcacga ctactacgtg
     1741 aaccgctaca tcgatctggg caacaccctg ttcagttgtg ccaccatgaa cgcgcacctg
     1801 gacagcctgc tggcgctgtt caccccggag atgccggcac aatgcgcgcg ctggggcacc
     1861 accatggccg catgggaggc gggtgtgcag caactgcgtg acttcatcaa cgcgcgttgc
     1921 gtcaccacgc agcagggcat ggtggactgc tacgacctgg aagggccgta caatgtgacg
     1981 ttggtggtgc aaccc
//
LOCUS       DAOQOC010000572         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38232_length_1993_cov_4.143447, whole genome shotgun sequence.
ACCESSION   DAOQOC010000572 DAOQOC010000000
VERSION     DAOQOC010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38232_length_1993_cov_4.143447"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1046)
                     /locus_tag="PL106_09435"
     CDS             complement(3..1046)
                     /locus_tag="PL106_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNA33332.1"
                     /translation="MNPASSTTPAPPALWVAMPIIRNIVLFAGNGPQGVETICAHGNL
                     AVEDLDNAELLLSLEQNCAIMDAALHISGDAHMGLHVGERTTASVLGLTGHIMQSSKD
                     ALSALMNVQQFTSAFSRLYVFRLERKGAETIYHCEPIPVWNDMSPETARHSVDIAFSA
                     VLHILFLLTGKRIRPLRANYRYLRLADLGEHERVLGCRPSFGQNANCIVFSTQDLTAP
                     IVGYNPQLNAMLKDLLDAEVRKQAGGASFSEQVRQVILRNVHVTFPALETIADVLHMT
                     PRTVQRKLQEEDTSFRIVCDTVKAEIARTLLTNPQLPISEIAFKLGYGEVSSFHRAFK
                     QWTGLTPVEYRKG"
     gene            1219..1932
                     /locus_tag="PL106_09440"
     CDS             1219..1932
                     /locus_tag="PL106_09440"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNA33333.1"
                     /translation="MQATDRYRNTIGLITILSGLLAAACIIVGAMAVENDFAAFSDPT
                     RTLLHAHNHVLAYWFNILDLFGYYLLLLPLIFHLHQLLKYRSPWMPLITFSGAAYVLV
                     GAIGASVLAAVWPSLMMDHLSAGPADQSAITAAFKTMTLAVTKGLWNILEVLFAAVWW
                     IGTGLLLRSASRPIAWLTIATGLSTLLDAIGNLLGLHLIAEIGLNLYLVLAIIWPIAI
                     GIRLMRGTPDVRHTNEAAQ"
BASE COUNT          339 a          669 c          596 g          389 t
ORIGIN      
        1 gcctacccct tgcgatactc caccggcgtt aaacccgtcc actgcttgaa cgcacggtgg
       61 aatgacgaca cttcgccata gcccagcttg aacgcgatct cgctgatcgg caattgaggg
      121 ttcgtcagga gcgtacgggc gatctccgcc ttcacggtgt cgcatacgat gcggaagctg
      181 gtatcctcct cctgcaactt gcgctgcacg gtgcgcgggg tcatgtgcag cacgtccgcg
      241 atggtctcca acgcggggaa ggtgacgtgg acgttccgca ggatcacttg cctgacctgc
      301 tcgctgaagg aggcgccacc ggcctgtttg cgcacttcgg catccagcag gtccttgagc
      361 atcgcgttga gctgcgggtt gtacccgaca atgggcgcgg tgaggtcctg cgtggagaag
      421 acgatgcagt tcgcattctg cccgaaggac ggccgacaac cgagcacccg ttcatgttcg
      481 cccaggtcgg cgaggcgcag gtagcggtag ttggcgcgca acggccggat gcgttttccg
      541 gtaagcagga acaggatatg gagcacggcg gaaaaggcga tgtccacgct gtggcgcgcg
      601 gtctcgggcg acatgtcgtt ccacaccggg atcggttcgc agtgatagat ggtctcggcc
      661 cccttgcgct ccagccggaa cacgtagagc cgcgagaacg cactggtgaa ctgttgcacg
      721 ttcatgagcg cggaaagcgc atccttgctg ctctgcatga tgtggccggt gaggccgagg
      781 accgatgcgg tggtccgttc gccgacatgc aggcccatgt gggcatcgcc ggagatgtgg
      841 agcgcagcgt ccatgatggc acagttctgc tccagggaga gcagcagttc cgcgttgtcc
      901 aggtcctcca ccgccaggtt gccatgcgcg cagatcgtct ccacgccttg cggcccgttc
      961 cccgcaaaga gcacgatgtt gcggatgatg ggcatggcga cccacagcgc cggtggcgcg
     1021 ggtgttgttg aactggctgg gttcatccgg atcgggcgga acgtgtatgc aataccgaag
     1081 atccgaaagt acgtggccgg accttcctgc cgtgcggccc gctgaccgcc ctggcggaaa
     1141 gtgtcaagat cctgtcgtga acggtcgatc ccgcagcagc acccgccggg aacatttgca
     1201 gccaccaaca cgaacgccat gcaagccacc gaccgctacc gcaacaccat cggcctcatc
     1261 accatcctct ccggcctgct ggccgccgcc tgcatcatcg tgggcgccat ggccgtggag
     1321 aacgatttcg ccgccttctc cgaccccacc cgcacgctgc tgcacgccca caaccatgtg
     1381 ctcgcctact ggttcaacat cctcgacctc ttcggctact acctcctgct ccttccgctg
     1441 atcttccacc tgcaccagct gttgaaatac cgctcgccgt ggatgccctt gatcaccttc
     1501 tccggcgcgg cttacgtgct ggtgggtgcc atcggtgcct ccgtgctggc cgccgtatgg
     1561 ccttccctga tgatggacca cctctccgcc ggacccgctg accagtccgc gatcactgcg
     1621 gctttcaaga ccatgaccct cgccgtgacg aagggtctgt ggaacatcct ggaggtcctc
     1681 ttcgccgccg tgtggtggat cggcacgggg ctgctgctgc gctccgcttc ccgccccatc
     1741 gcctggctca ccatcgccac gggcctctcc acgctgctgg acgcgatcgg caacctcttg
     1801 ggccttcacc tgatcgccga gatcggcctg aacctctacc tggtgctggc gatcatttgg
     1861 cccatcgcca tcggtatccg gttgatgcgc ggcacgcccg atgtgcgcca tacgaacgaa
     1921 gccgcacagt agtcaccgtt ccccgaaact ccttacgccc atgtacaccc tgctccgtat
     1981 cctgaagtgg acc
//
LOCUS       DAOQOC010000573         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38379_length_1990_cov_2.193798, whole genome shotgun sequence.
ACCESSION   DAOQOC010000573 DAOQOC010000000
VERSION     DAOQOC010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38379_length_1990_cov_2.193798"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PL106_09445"
     CDS             complement(<1..204)
                     /locus_tag="PL106_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33334.1"
                     /translation="MRVVHNDQVLGPTSDAPDDIEDRIARLRGVYEKLDDKCRAILDQ
                     FYYERKDLATIAASMGVEEESIRT"
     gene            complement(201..515)
                     /locus_tag="PL106_09450"
     CDS             complement(201..515)
                     /locus_tag="PL106_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33335.1"
                     /translation="MAQLDTGTRPEQDTAWLDGLRRNDPVLMRSLYKTHFPAIRQFVL
                     QNSGRPSDAQDIFQEAVIVLWMSAKEGRLTMERRTAPADSSSGWRRTNGWTWSGPQRT
                     ST"
     gene            703..1164
                     /locus_tag="PL106_09455"
     CDS             703..1164
                     /locus_tag="PL106_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA33336.1"
                     /translation="MEIPLHTCPAGLATGERSFRLMLALVPAGEWPSPGDPERSVGYI
                     LQHGEPVELLAALRAMANGNIPGPERIGRRVSFRMLPDTGGATGDTRLTGREADVLRG
                     LVDGLSYKMVADHLGISFETVRSHVKRVYKKLSVRSSTEAVAKALKSGLVA"
     gene            1407..>1990
                     /locus_tag="PL106_09460"
     CDS             1407..>1990
                     /locus_tag="PL106_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33337.1"
                     /translation="MNRKLLTVGTVFGLLAGELSAQLPRIVVQGTGGPAVFTSLDTAL
                     SSAQPGDKVYLSGGSFLQVGDLVIDKQLHLIGAGIMPDSANVTATTQIHITNNLRFTT
                     PCSGSTFTGISFFPDDHTYFGTNPDDDDPVGLVFQRCEFVRGAINLGSDGNSVGSATF
                     DECIMHTTGTNQGFNGARGEAVLTRCILNHKTLSG"
BASE COUNT          378 a          627 c          609 g          376 t
ORIGIN      
        1 cgttcggatg ctctcttcct ccacacccat gctggccgcg atggtggcca ggtccttccg
       61 ttcgtagtag aactggtcca ggatggcgcg gcacttgtcg tcgagtttct cgtacacacc
      121 gcgcaggcgt gcgatgcgat cctcgatgtc gtcgggcgcg tcgctggtgg ggccgagcac
      181 ctggtcgtta tgcaccactc tcatgtgctt gtgcgctgcg gaccggacca cgtccagcca
      241 tttgttcttc gccacccgga agaggaatcc gccggggccg ttctccgttc catggtgagc
      301 cggccttcct tggcgctcat ccacagcacg atcacggcct cctggaaaat gtcctgtgca
      361 tcgcttggcc ggccgctgtt ctggagcacg aactgccgga tcgccgggaa atgcgtcttg
      421 tacaaggacc gcatcagcac cggatcgttg cgtcgcaacc cgtccagcca agcggtgtcc
      481 tgttcggggc gcgtccccgt gtcgagctgg gccatgcgaa ggtgcgtggc agcgaatatc
      541 gacaaaccgg tcggtatggt acatgacttt gagcatgagg ccgggatcgg gccttcaggg
      601 agcatatcgg agacggtgcg ggaatgtgaa caacccgaag atccgcgttt ggatcgttca
      661 catcgcgcag gtacccccga tatggggatg aacaacgatc acatggagat ccccttgcac
      721 acctgtcccg caggcctcgc caccggcgag cgttcattcc ggctcatgct cgccctggtc
      781 cccgctgggg aatggccatc acccggcgac ccggagcgct ccgtgggcta tatactgcaa
      841 cacggggagc cggtcgagtt gttggccgct ctgcgcgcca tggccaacgg gaacattccc
      901 gggccggaac gcatcggtcg gcgagtgagt ttccgcatgt tgcccgatac gggtggcgcc
      961 actggggata cccggctgac agggcgtgag gccgatgtgc tgcgcggtct ggtggacggg
     1021 ctcagctaca agatggtggc ggaccacttg gggatcagct tcgagacggt gcgcagccac
     1081 gtgaagcggg tgtacaagaa actcagtgtg cgcagcagca ccgaagccgt ggccaaagcg
     1141 ctgaaaagcg gcttggtcgc ctgaggtgtg ctaccatttg cgcattcaag gctggccaag
     1201 agggacgcca cgatccttcc gctctccagc gatgctgcta cgagcatccc acggaaatag
     1261 gatatatacg atgcccattc gccaatgtat ccgcaggacg cacggctcgg tatcttgtgg
     1321 gcaaaactgt acatgctgtt cacatttccg ggctgacccc gatgtacgcc acgaagaaca
     1381 tcccaaccct tgaacctcct tcaccaatga acagaaagct actcaccgtc ggaacggtat
     1441 tcggactgct ggccggcgaa ctcagcgccc aactgccgcg catcgtggtg cagggcaccg
     1501 gcggccctgc ggttttcacc agcctggaca ccgctctgtc cagcgcacaa ccgggcgata
     1561 aggtctacct gagcggaggg agcttcctgc aggtcggcga cctggtgatc gacaagcagc
     1621 tgcacctcat cggtgcaggg atcatgcccg atagcgccaa tgtgaccgcc accacgcaga
     1681 tccacatcac gaacaacctc cggttcacca cgccgtgctc gggaagcacc ttcacgggga
     1741 tctccttctt ccccgatgac catacctatt tcggcacgaa cccggatgat gacgatccgg
     1801 tcggtctggt gttccaacgc tgcgagttcg tccgaggcgc gatcaacctg gggagcgatg
     1861 gcaattcggt cgggtcggcc accttcgatg agtgcatcat gcacacgacc gggacgaacc
     1921 agggctttaa cggcgcgcgc ggagaggccg tgctgacccg ctgcatcctc aaccacaaga
     1981 ccctcagcgg
//
LOCUS       DAOQOC010000574         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38404_length_1989_cov_3.456567, whole genome shotgun sequence.
ACCESSION   DAOQOC010000574 DAOQOC010000000
VERSION     DAOQOC010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38404_length_1989_cov_3.456567"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PL106_09465"
     CDS             complement(<1..327)
                     /locus_tag="PL106_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33338.1"
                     /translation="MAHRLTLDGGTIAPDLMAKLYANINSRKEPLVLERATPHAPWTI
                     NGRAGDLVRTGPGQYSLVLDGRSHRVQVLKEDKENGILRIRIGAKTYNVRLEDEQAHL
                     MQSLGLD"
     gene            complement(364..837)
                     /gene="rnhA"
                     /locus_tag="PL106_09470"
     CDS             complement(364..837)
                     /gene="rnhA"
                     /locus_tag="PL106_09470"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNA33339.1"
                     /translation="MRVTAYTDGAASGNPGPGGYGAVLEAGKHRRELWGGYRRTTNNR
                     MELMAVIVALEALKLPGMEVTVVSDSKYVVDAVEQGWLFDWEKKGFAKKKNPDLWKRF
                     LAIYRQHKVRFEWIRGHNGHPQNELCDRLAVAAREQPGLPPDEAYERTGEDLFSE"
     gene            complement(834..1451)
                     /locus_tag="PL106_09475"
     CDS             complement(834..1451)
                     /locus_tag="PL106_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNA33340.1"
                     /translation="MFSLTEIGKAFLILFAVIDVVGSIPVILDVRQKAGKIYPARATL
                     VSLGIMVSFLYLGETILRVVGIDVRSFAVAGSLVLFFLALEMTLGVKLFKEDLSTPGL
                     KDGRADNKVYSIVPLAFPVIAGAGTITTILSLRAEFERPNILVAILLNIVVVVVVLLL
                     TNRIERMLGRLGLIVIKKAFGIILLAISVKLFAGNITYLFPGINP"
     gene            complement(1503..>1989)
                     /locus_tag="PL106_09480"
     CDS             complement(1503..>1989)
                     /locus_tag="PL106_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HNA33341.1"
                     /translation="RVLLSIADELEQRNAGFEHDEKRVHVARIRALTEEVPPLPDFSS
                     FHRSFQGLPGVPGGDLRAAYFLGYKEERCRHVKLHEALDELRRGGELVSASFVIPYPP
                     GFPVLVPGQTINKDIIDFLIAIDVKEIHGYRPEMGLRVFTDAALGRQKTGTALGRMAR
                     K"
BASE COUNT          354 a          624 c          619 g          392 t
ORIGIN      
        1 gtccagaccg agcgactgca tcaagtgcgc ttgctcatcc tccagccgca cgttataggt
       61 cttcgcgccg atgcggatac gcaggatgcc gttctccttg tcctccttca gcacctgtac
      121 gcgatggcta cgcccatcca gcaccaagct gtactgcccc ggtccggtgc gtaccaggtc
      181 gcccgcgcgc ccgttgatgg tccaaggtgc gtggggtgtt gcacgctcca ggaccagcgg
      241 ttccttccgc gaattgatgt tggcgtacag tttggccatg aggtccggcg ctatcgttcc
      301 accgtcgagg gtcaggcggt gcgccaaggt aacgggtcgg tgcgttctgt ccccggcgcg
      361 gagctattcg ctgaagaggt cttcacccgt ccgttcgtag gcctcgtcgg ggggcaggcc
      421 cggttgttcg cgtgcggcca cggcgagcct gtcgcacagt tcgttctgcg gatgtccgtt
      481 gtgaccgcgg atccactcga agcgcacctt gtgttggcgg tagatggcca ggaagcgttt
      541 ccacaggtcg gggttcttct tcttggcgaa gccctttttt tcccagtcga agagccagcc
      601 ttgctccacc gcatccacca cgtacttgct gtcgctcacc acggtcacct ccatgcccgg
      661 gagtttcagg gcttccagcg ctacgatcac ggccatcagt tccatgcggt tgttggtggt
      721 gcggcgataa ccgccccaca actcgcgccg gtgtttgccg gcctccagca cggcaccgta
      781 gccgcccggc ccgggattac cgctggcggc gccatccgta taggccgtta ccctcatgga
      841 ttgatccctg ggaaaaggta tgtgatattc ccggcgaaca gcttcacact gatggcgagc
      901 aggatgatgc cgaacgcttt cttgatcacg atgaggccca ggcgccccag catccgttca
      961 atgcggttgg tgagcagcag gaccaccacg accacgacga tgttgagcag gatggccacc
     1021 aggatgttgg gtcgttcgaa ctcggcgcgc aagctgagga tggtggtgat ggtgcccgca
     1081 ccggcgatca ccggaaaggc caggggcacg atgctgtaca ccttgttgtc cgcccggcca
     1141 tccttcaggc ccggtgtgga aagatcctcc ttgaagagtt tcacaccgag ggtcatctcc
     1201 agcgcgagga agaagagcac caaggagccc gccacggcga aggagcgcac atctataccc
     1261 acgacacgga ggatcgtttc cccgaggtag aggaagctga ccatgatgcc gaggctcacc
     1321 aaggtggccc gtgcgggata gatcttgccc gccttctgcc gcacgtcgag gatcacgggg
     1381 atgcttccca ccacgtcgat caccgcgaag aggatcaaaa aggccttgcc gatctcggtc
     1441 agggagaaca tggcgcaaag gaacgccggg cccgacatac acgcatgggc cgcgccggtg
     1501 gatcacttcc tggccatgcg gcccagtgcg gtgccggtct tctgcctgcc cagggccgca
     1561 tcggtgaaca cacgcaggcc catttcgggg cggtaaccgt ggatctcctt cacgtcgatg
     1621 gcgatgagga agtcgatgat gtccttgttg atcgtctggc ccgggaccag cacggggaat
     1681 cccggtggat aggggatcac gaagctggcc gataccaatt caccaccgcg ccgcaattca
     1741 tcgagtgctt cgtgcaattt cacgtggcgg cagcgctcct ccttgtagcc caggaagtat
     1801 gcggcgcgca gatcaccgcc gggtacgccg ggaagccctt ggaagctgcg gtggaagctg
     1861 ctgaaatccg gcaggggggg cacctcctcg gtgagcgccc tgatgcgcgc cacatgcact
     1921 cttttctcat cgtgctcgaa cccggcgttg cgctgttcca attcatcggc gatgctgagc
     1981 agcaccctg
//
LOCUS       DAOQOC010000575         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38430_length_1988_cov_5.082773, whole genome shotgun sequence.
ACCESSION   DAOQOC010000575 DAOQOC010000000
VERSION     DAOQOC010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38430_length_1988_cov_5.082773"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1487
                     /gene="sufB"
                     /locus_tag="PL106_09485"
     CDS             39..1487
                     /gene="sufB"
                     /locus_tag="PL106_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNA33342.1"
                     /translation="MAQDTNDILREVTEKEYAYGFVTNIESEKAEKGLNEDIVRFISK
                     KKEEPEWMLEWRLEAFRLWQKMEEPTWAHVNYPKIDYQDAYYYSAPKKKPQLNSLDEA
                     DPELVKTFEKLGISLEEQKRLAGVAVDVVFDSVSVKTTFQKTLKEKGIIFCSFSEAVR
                     EHPDLVKKYIGSVVPRTDNYFAALNSAVFSDGSFCYIPPGVRCPMELSTYFRINEAMT
                     GQFERTLLIADEGAYVSYLEGCTAPIRDEHQLHAAVVELVALKDAEIKYSTVQNWYPG
                     DKDGKGGIYNFVTKRGLCLGDRSKISWTQVETGSAITWKYPSCVLKGDQSTGEFYSVA
                     VTNNRQQADTGTKMTHIGKGTKSTIVSKGISAGYSNNSYRGLVKIGRGAKGARNFSQC
                     DSLLLGDRCGAHTFPYIESENPNAMVEHEATTSKIGEDQIFYLNQRGIETEKAVSLIV
                     NGYCKEVLNQLPMEFAVEAQKLLAVSLEGSVG"
     gene            complement(1655..1915)
                     /locus_tag="PL106_09490"
     CDS             complement(1655..1915)
                     /locus_tag="PL106_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA33343.1"
                     /translation="MAFWCDLAADAAIGPFVKFAKTVAQYWAGITNHTKYHFSNGIRE
                     GINSKVQLARARARGYLNIQNIINVIYFLTGKLKFDYPLYST"
BASE COUNT          466 a          553 c          600 g          369 t
ORIGIN      
        1 attctccgtg tcctctgcgc ctctgtggtg aagcattcat ggcacaggac accaacgata
       61 tcctccgtga ggtcaccgaa aaggagtacg cctacggttt tgtcacgaac atcgagagcg
      121 agaaggccga gaagggcctg aacgaggaca tcgtacgctt catcagcaag aagaaggaag
      181 agccggagtg gatgctggag tggcgcctcg aggccttccg gctctggcag aaaatggaag
      241 agcccacctg ggcgcatgtg aactatccga agatcgatta ccaggacgcc tattactaca
      301 gcgcgcccaa aaagaaaccg caactcaaca gcttggacga ggccgacccc gagctggtga
      361 agaccttcga gaagctgggc atctccttgg aggagcagaa gcgccttgcc ggtgtggccg
      421 tggatgtggt gttcgacagc gtgagtgtga agaccacttt ccagaagaca ttgaaggaaa
      481 aagggatcat cttctgctcg ttcagcgagg cggtacggga acatcctgat ctggtaaaga
      541 agtacatcgg cagcgtggtg ccacgcacgg acaactactt cgccgcgctc aacagcgccg
      601 tgttcagcga tggcagcttc tgctacatcc cgccaggagt acgctgtccc atggagttga
      661 gcacctactt ccgcatcaac gaggccatga ccggccagtt cgagcgcacg ctgctgatcg
      721 ccgacgaggg cgcttacgtg agctacctgg aaggatgcac cgcccccatc cgcgatgagc
      781 accagttgca cgccgccgtg gtggaactgg tcgcgctgaa agacgcggag atcaagtaca
      841 gcaccgtcca gaactggtac cccggcgaca aggacggcaa gggcggcatc tacaatttcg
      901 tcaccaagcg tggcctttgc ctcggcgatc gcagcaagat cagctggacg caggtggaga
      961 ccggatcggc gatcacctgg aagtacccga gctgcgtact caagggcgac cagagcaccg
     1021 gcgagttcta cagcgtagcg gtgaccaata atcggcagca ggccgacacg ggcaccaaga
     1081 tgacccacat cggcaagggc accaagagca ccatcgtgag caagggcatc agcgcgggtt
     1141 attccaacaa cagctaccgt ggactggtga agatcggtcg cggcgcgaaa ggcgcacgga
     1201 acttcagcca gtgcgattcc ctcctgctcg gcgaccgctg cggcgcacac accttcccgt
     1261 acatcgagag cgagaacccc aacgccatgg tggagcacga ggccaccacc agcaagatcg
     1321 gtgaggacca gatcttctac ctcaaccagc gcggcatcga gaccgaaaaa gcggtatcac
     1381 tcatcgtgaa cggctactgc aaggaggtgc tgaaccaact gccgatggag ttcgccgtgg
     1441 aggcgcagaa gttgttggcg gtgagtctgg aggggagcgt ggggtgatca gccaacgttc
     1501 atgaccgtgt tccggccgac ggctgtcgcc atttcatcct atgcggccat cggcccccaa
     1561 tggtcatctg cttggaactg ctccaggacc aagcgccgca atttggccaa agccttgcac
     1621 ttggctgtct tcgccgaatt gacactggga ggctctatgt cgaatacagt gggtagtcga
     1681 acttgagctt accggtgagg aagtatatca cgttgatgat gttttggata ttcaggtagc
     1741 cccttgccct tgctctggcg agttggacct ttgaattgat cccttcgcgg atcccgttgg
     1801 agaagtggta cttggtgtgg ttggtgatgc cagcccaata ctgggcaacg gtcttcgcga
     1861 acttgacaaa agggccaatg gctgcatcgg cggccaagtc acaccagaag gccaggaagc
     1921 tcctgtttta actaaatggg tcacccaaag tcgataccga aggttctgag caggagttgt
     1981 tggtcttg
//
LOCUS       DAOQOC010000576         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38480_length_1987_cov_2.798654, whole genome shotgun sequence.
ACCESSION   DAOQOC010000576 DAOQOC010000000
VERSION     DAOQOC010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38480_length_1987_cov_2.798654"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PL106_09495"
     CDS             <1..547
                     /locus_tag="PL106_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNA33344.1"
                     /translation="VMFTHDVIMSDNHFEVNRGASAYGLLLKEINDATITGNVFTDNT
                     VGILIDGCNRAQVKDNLFHANGWAVRLLANSLDCVFDGNSFRSNTFDMTTNGDLVKST
                     LRNNYWDRYRGYDLDHDGRGDVPHRPMSVFGAVTERAPYAMVLSRSLMVDLLDQAERI
                     VPTLTPAALEDRSPLMHPPHG"
     gene            540..1277
                     /locus_tag="PL106_09500"
     CDS             540..1277
                     /locus_tag="PL106_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA33345.1"
                     /translation="MDRIVIEGLGKRYGRQWALRGVNARFVPGEVAMVIGPNGSGKTT
                     LIKCLLGLTRATEGAITVNGTVIGPDPAYRKAIGYMPQLAEFPKELTVEQLLRMMNDI
                     RDARSGATDDRLIGELGVAALLDKRIGTLSGGMRQKVSAVLAFRYRPDILVLDEPTAG
                     LDPVSAGRLLNAVIRARREGATVLITSHIMEEVQRLGDRVVYLQDGRLRFTLAPETIT
                     AATGEPALSQALPKYISQLATSDVEGL"
     gene            1261..>1987
                     /locus_tag="PL106_09505"
     CDS             1261..>1987
                     /locus_tag="PL106_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA33346.1"
                     /translation="MWKVCKYTLLDLARNRFALGYTMMLFAVSTGMFMIEGDNTKALI
                     TLSQVVLALVPLLALVFTIIYFYNQYEFTVLLAVQPISRRSILMSHWLAVSTALLAAF
                     MIGVGLPVLAWAPGAVGAALLLSGTALTAVFCGLGIWIAVVQRDRARAVGIGLVTWVL
                     MVLVYDSMLLWLMFAFSDRPIEPLIVPLAALNPIDLSRMLIMLKIDLAALLGYTGAVY
                     HRFFGSVGGMLMAALILLLWVALS"
BASE COUNT          362 a          601 c          630 g          394 t
ORIGIN      
        1 ggtgatgttc acccacgacg tgatcatgtc cgacaaccac ttcgaggtga accgcggagc
       61 cagcgcatac ggattgctgc tgaaggagat caacgatgcg accatcaccg ggaatgtgtt
      121 caccgacaac acggtgggca tcctgatcga cgggtgcaac cgcgcgcaag tgaaggacaa
      181 tctcttccac gccaacggct gggccgtgcg gttgctggcc aacagcctgg attgcgtgtt
      241 cgatgggaac agcttccggt cgaacacctt cgacatgacc accaatggcg acctggtgaa
      301 gagcacgttg cggaacaatt actgggaccg ctatcggggc tacgacctgg atcatgacgg
      361 gcgtggcgat gttccgcacc gccccatgag cgtgttcgga gcggtgaccg aacgggcacc
      421 atacgccatg gtgctctcac gcagcctgat ggtggatctg ctggaccagg ccgaacgcat
      481 cgtgcccacg ctcacgcccg ccgctctcga ggaccgatca cccctgatgc acccgcccca
      541 tggataggat cgtgatcgaa ggcctgggaa aacgttacgg tcgccaatgg gccttgcgtg
      601 gcgtgaatgc gcggttcgtg cccggtgagg tggccatggt gatcggcccg aacggatcgg
      661 gcaagaccac cctgatcaag tgcctgctcg gattgacgcg cgccacggaa ggcgcgatca
      721 cggtgaacgg aacggtgatc gggccggatc cggcctatcg caaggcgatc ggctacatgc
      781 cgcagctggc cgagttcccg aaggaactga ccgtggagca gttgctgcgc atgatgaacg
      841 acatccgtga cgcacgatcc ggcgccaccg atgaccggtt gatcggcgag ctgggtgttg
      901 cagccctgct ggacaagcgg atcggtacct tgagcggggg catgcggcag aaggtgagcg
      961 ctgtactggc tttccggtac aggcctgaca tcctcgtgct cgacgagccc accgccgggc
     1021 tggacccggt gagtgcgggc cgtttgctga acgccgttat acgtgcgcga cgcgaagggg
     1081 ccaccgtgct catcacctcc cacatcatgg aggaggtgca gcgcctgggc gatcgcgtgg
     1141 tctacctgca ggatggccgg ttgcgattca ccttggcacc ggaaacgatc accgcggcca
     1201 ccggggaacc ggccctctca caagccctgc ccaagtacat ctcacaactc gcaacaagcg
     1261 atgtggaagg tctgtaaata caccctgctc gatctggcgc gcaaccggtt cgccctcgga
     1321 tacacgatga tgctcttcgc ggtctccacc ggcatgttca tgatcgaggg cgacaacacc
     1381 aaggcattga tcacactctc gcaagtggtg ctcgcgctcg ttccgttgct cgcactggtg
     1441 ttcaccatca tctatttcta caaccagtat gagttcaccg tacttctggc ggtgcagccg
     1501 atcagcaggc gatcgatact gatgtcccat tggctggcgg tgagcacagc attgctggcc
     1561 gcgttcatga tcggtgtggg acttccggtg ctggcttggg cacctggggc ggtgggcgcc
     1621 gcgcttctgc tgtcgggcac cgcgcttacc gcggtattct gcggccttgg gatatggatc
     1681 gccgtggtgc aacgcgatcg cgcgcgggcg gtggggatcg gtctggtgac ctgggtgctg
     1741 atggtgctcg tgtacgattc aatgctcctg tggttgatgt tcgccttcag cgaccggccc
     1801 atcgaaccgt tgatcgttcc attggcggcg ctcaacccga tcgacctctc gcgcatgctc
     1861 atcatgctga agatcgatct ggccgcgctg ctcggctata ccggtgcggt ataccaccgg
     1921 ttcttcggaa gcgtcggcgg catgctcatg gccgctttga tcctgctcct ttgggtagca
     1981 ttgtcca
//
LOCUS       DAOQOC010000577         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38496_length_1986_cov_3.988089, whole genome shotgun sequence.
ACCESSION   DAOQOC010000577 DAOQOC010000000
VERSION     DAOQOC010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38496_length_1986_cov_3.988089"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1986)
                     /locus_tag="PL106_09510"
     CDS             complement(<1..>1986)
                     /locus_tag="PL106_09510"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33347.1"
                     /translation="VCDIPPHIRPSACNPTGTNACDGGSLNSLHLFNYMNYTTCTDNM
                     FTADQRTRAQLAMTSERASFLASNGNMSLVPPGAPQLDFRASASVLCGTGQSVTMEDV
                     SSCLPNTYLADAEFPGISFAWTLTNGIFTYNSTFRRPTFIVNGTGVYNATLTVTTSLG
                     TYTRTENGIVVVAGTPVAACTPTSSNECNCAQTVNNVVFGSISNATSTGTNVAYTDFT
                     CTRNTVVASGGTYPLAVSIRAGGSAAEVLNGYIDWNNNGVFEDPSERVIQGSTPANSS
                     TTVNANVTVPGGAVTNTLLRMRMYGEAGTFTSAERTCGAAFFVGDVEDYGVYVSSNVA
                     GVSIAASPGSTITYGTNVTFTPTPVNGGASPVYTWFRNGINVGNGATYQSNNLMPGTT
                     IRCEMASNMANVLSSPALSNTITMTVTGPPLTEFSGTPVALCAGGTVTYTDASLLSPT
                     SWSWSFPGGTPSTSTAQNPVITYNTPGTYNVTLVASNANGTGTTMTKTGYINVFAAPV
                     AACTHTRTSAPGSGIGITNVTVNTIDNNTVYDGAAMNNYTCAQTTGLQINTTYAISIT
                     GGTFNNQWFRAYIDYNNNGVFTDTGEQVFAPANAMGTVSGNFTTPSSPTLNTLLRMRV
                     ISDFVNTTPGPCTNLQYGQSEDYGIYFIPPNCTPPTAT"
BASE COUNT          357 a          565 c          655 g          409 t
ORIGIN      
        1 gtggccgttg gcggcgtgca attcggcggg atgaagtaga tgccgtagtc ctccgactgg
       61 ccgtactgga ggttcgtgca ggggccgggc gtggtgttca cgaagtcgct gatcacccgc
      121 atgcgcaaca gggtattcaa ggtggggctc gatggcgtgg tgaaattgcc cgataccgta
      181 cccatggcat tggccggtgc gaaaacctgc tcgcccgtat cggtgaagac cccgttgttg
      241 ttgtagtcga tgtacgcccg gaaccactgg ttgttgaacg taccgcccgt gatcgagatg
      301 gcgtaggtgg tgttgatctg cagcccggtc gtctgagcgc aggtataatt gttcatcgcc
      361 gcaccatcat aaacggtgtt gttgtcgatg gtgttcaccg tgacgttggt gatgccgatg
      421 cccgagcccg gcgccgaggt ccgcgtatgt gtacaggccg ccacgggagc cgcgaaaacg
      481 ttgatgtagc ccgtcttggt catggtggtg cccgtaccgt tcgcattgct cgcgaccaat
      541 gtcacgttgt acgtgccggg cgtgttgtac gtgatcaccg ggttctgcgc ggtggatgtt
      601 gaaggcgtcc ccccggggaa cgaccacgac cagcttgtcg gtgaaagcaa cgaggcatcc
      661 gtatacgtca cggttcctcc ggcgcacagg gccacgggag tcccactgaa ctccgtgagc
      721 ggtggaccgg taacggtcat ggtgatcgtg ttcgacagcg cgggcgagga aagaacgttg
      781 gccatgttcg acgccatttc gcagcggatg gtggtgcctg gcatcaggtt gttgctctgg
      841 taggtggcgc cattccccac attgatcccg ttgcggaacc aggtgtacac gggcgatgcc
      901 ccgccgttca ccggggtggg cgtgaaggtg acgttcgtgc cgtacgtgat ggtactgccc
      961 ggcgaagccg cgatcgagac acccgccaca ttgctcgaga catacacacc gtagtcctcc
     1021 acatcgccaa cgaagaaggc ggcaccgcac gtccgttcag cggatgtgaa cgtaccagcc
     1081 tcaccgtaca tgcgcatacg caggagggta ttggtcaccg ccccaccggg caccgtgacg
     1141 ttcgcattca ccgtggtgct ggaattcgcc ggtgtcgagc cctgaatgac gcgctcgctt
     1201 ggatcctcga acacaccgtt gttgttccaa tcgatgtacc cgttgagcac ctcggccgcg
     1261 ctgcctcccg cacggatgga aacggcgagc ggataggtgc cacccgacgc gaccaccgtg
     1321 ttgcgcgtgc aggtgaagtc cgtgtacgcc acattggtcc cggtgctggt agcgttggag
     1381 atggaaccga aaaccacatt gttcaccgtc tgggcgcagt tgcattcgtt gctcgatgtc
     1441 ggtgtgcagg cggccaccgg cgtgccggcc acaaccacga tcccgttctc cgtgcgggtg
     1501 tacgtgccca acgaggtggt caccgtcagc gtcgcattgt acacgcccgt gccgttcacg
     1561 atgaaggtcg gcctgcggaa ggtcgagttg tacgtgaata tgccgttggt gagggtccag
     1621 gcgaagctga tcccggggaa ttcagcatcg gccaggtagg tgttcggcag gcaggaggaa
     1681 acatcctcca tagttaccga ttgaccggta ccgcacaaca ccgatgccga tgcccggaaa
     1741 tcgagttgtg gcgcgccggg cggcaccagt gacatgttgc cgttactggc caggaacgaa
     1801 gcgcgctcgc tggtcatggc gagctgcgcg cgcgtccgct gatccgcggt gaacatattg
     1861 tcggtacacg tggtgtagtt catgtagttg aacaggtgca ggctgttcaa tgatccacca
     1921 tcgcacgcgt tggtcccggt ggggttgcac gcgctcggac ggatgtgcgg cggtatgtcg
     1981 cacaca
//
LOCUS       DAOQOC010000578         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38503_length_1986_cov_3.106163, whole genome shotgun sequence.
ACCESSION   DAOQOC010000578 DAOQOC010000000
VERSION     DAOQOC010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38503_length_1986_cov_3.106163"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PL106_09515"
     CDS             <1..1747
                     /locus_tag="PL106_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNA33348.1"
                     /translation="GICGLFGIHFNAVNILVCTFVFGLGDDYCIFTSEGLLARFRTGE
                     DHTRSFRGAVILSAVTTIIGTGVLVFAEHPALRSIAFLSVAGMAALLVISLTVQPALF
                     HLLIAGRAANGKQPFTLLSLTISLFAFLYFLAGCLLLSALWLLFLVLPAPGRIKQHWT
                     RRIIGLFTGSLVYVMVNVRKDIRGFRAAVKDRPAILVANHNSFIDILAMLMITPEAVM
                     MTNRWVWNSPFFGRVVRYAGYLCTDDGQEANVEKARGLMAQGLSIIIFPEGTRSKDGT
                     IGRFHKGAFQMAEALNVPIIPVVLHGFGEAMGRSDALLKNTTISMRTLPAIMPDEPRF
                     GQGYRARTKAISHWFKEEYEKIRAARETPSWFHERLVRNFTYKGPVIEWYTRVKARMD
                     RDLHELLHARIARDATVVDLGAGHGMVSRLLYWSAPARRIMAFERDEEKVLLARHCYS
                     KDEGITFNQADLRDLVPPPADAYVIKDVLHYMDPTAQRALLLACARNLRPGGSILLRD
                     GFSAPGARHVRTRWTERLSTGIGFNKTSGELHFMAKDTLLAIAAEAGLAVEWALAEAR
                     TSNELAILSATDARP"
     gene            1734..>1986
                     /locus_tag="PL106_09520"
     CDS             1734..>1986
                     /locus_tag="PL106_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA33349.1"
                     /translation="MHDREFDVVIVGAGLGGLQCAVTLAREGMSVCVLEMNQQLGGSL
                     QVFSRNKTVFDTGVHYIGGLEPGQNLHRYFSYYGIMDKLK"
BASE COUNT          341 a          619 c          617 g          409 t
ORIGIN      
        1 tggtatctgt ggtcttttcg gtatccactt caatgccgtg aacatcctgg tctgcacctt
       61 cgtgttcggc ttgggcgatg attactgcat cttcaccagc gagggtctcc tcgcccgttt
      121 ccgcactggc gaggaccaca cgcgttcatt ccgtggggcg gtgatcctgt cggccgtcac
      181 gacgatcatc ggtaccggcg tgctggtgtt cgcggagcac ccggccctgc gttccatcgc
      241 cttcctctcc gtggcgggca tggccgccct gctcgtcatc tcgctcacgg tgcaacccgc
      301 cctgttccac ctgctcatcg ccgggcgcgc ggccaatggc aagcaaccct tcaccctgct
      361 ctcgctgacc atctcccttt ttgctttttt atactttttg gcaggttgtc ttctgctctc
      421 ggccctgtgg ttgcttttcc tggtgctgcc cgctcccggg cggataaaac agcattggac
      481 gcgccgcatc atcggcctgt tcaccggctc gctcgtgtac gtcatggtga acgtgcgcaa
      541 ggatatccgg ggcttccgcg cggcggtgaa ggaccgcccc gccatcctgg tggcgaatca
      601 caactccttc atcgacatcc tcgccatgct catgatcacc ccggaagcgg tgatgatgac
      661 caaccgttgg gtgtggaaca gccccttctt cggccgcgtg gtgcgctacg ccgggtacct
      721 ctgcacagac gatgggcagg aggccaatgt ggaaaaggcc cgtgggttga tggcacaggg
      781 actctcgatc atcatttttc cggaaggcac ccgatcgaag gacgggacca tcggccggtt
      841 ccacaagggg gccttccaga tggccgaagc gctcaatgtt cccatcatcc cggtggtgct
      901 gcacggtttc ggcgaggcta tgggcaggag cgatgccctg ttgaagaata cgaccatctc
      961 catgcggaca cttccggcga tcatgcctga tgaaccgcgc ttcgggcagg ggtatcgggc
     1021 ccggacgaag gccatcagcc attggttcaa ggaggagtat gaaaaaatac gtgcggcacg
     1081 ggagacgcca tcctggttcc atgagcggct cgtccgcaac ttcacgtaca agggcccggt
     1141 gatcgaatgg tacacgcgtg tgaaggcccg catggaccgg gacctgcacg aattgctgca
     1201 tgcccgcatc gccagggacg ccacggtggt cgatctgggt gcggggcacg gcatggtgag
     1261 ccgcctgctg tactggagtg cgcccgcccg gcggatcatg gctttcgagc gtgatgagga
     1321 gaaggtgctg ctggcccggc actgttacag caaggatgag ggcatcacgt tcaaccaggc
     1381 ggaccttcgg gacctcgtgc ccccgcccgc ggatgcgtat gtgatcaagg acgtgctgca
     1441 ctatatggac ccgaccgcgc agcgcgcctt gctgctggcc tgtgcgcgca acctgcgtcc
     1501 cggtggctcc atcctgctgc gcgatggctt ctcggcgccc ggggcccggc acgtacgcac
     1561 gcgctggacc gagcggctct ccacaggcat cggattcaac aagacctcgg gcgaattgca
     1621 cttcatggcg aaggacacct tgctggccat tgccgccgaa gcgggtctcg ccgtcgaatg
     1681 ggctctggcc gaggcccgta catccaacga actggccata ctttccgcaa ccgatgcacg
     1741 accgtgagtt cgatgtggtg atcgtgggcg caggcctcgg gggtctgcaa tgcgccgtta
     1801 ccctggcccg cgagggcatg agcgtttgcg tgctggagat gaatcagcag ctcgggggaa
     1861 gcctgcaggt cttcagccgg aacaagaccg tttttgacac cggtgtgcac tacatcggtg
     1921 gactggagcc aggacagaac ctgcaccgct acttcagtta ttacggcatc atggacaagc
     1981 tgaagc
//
LOCUS       DAOQOC010000579         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38542_length_1985_cov_2.590674, whole genome shotgun sequence.
ACCESSION   DAOQOC010000579 DAOQOC010000000
VERSION     DAOQOC010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38542_length_1985_cov_2.590674"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PL106_09525"
     CDS             <1..851
                     /locus_tag="PL106_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNA33350.1"
                     /translation="RQQWRSVTVPYRTFGIGGDMADVAGLKGLGAGLRLYNDRAGDSR
                     LNTFHLSAAVSWTQRFGDHHAQSITGGIQAGFTSLSIDYSALRYDAQYNGYYFDPSLS
                     NNEQFTRDAATHPDLHAGLIYRYAPDKRRSIQAGIALFNLTTPRIDLNGDLSPLDRRT
                     ALHVTTRFPVSAKLDVQPMAQYMAQATFRELLLGGSVRYILKDKYTYLRTVQAGLFWR
                     AADAGMVFAGLEIDDWTAGLSYDLNFSKLVPASRNRGGFEITVIRVFRKKPFVPVRYK
                     ACPTQL"
     gene            863..>1985
                     /locus_tag="PL106_09530"
     CDS             863..>1985
                     /locus_tag="PL106_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33351.1"
                     /translation="MRQRTRTGVRGPLVMLVCLLVRSSCAQNAAQFVTWGDNAMAIED
                     YYGASKFYAQAMEMEGGRLELQWKYAEAGRLSNQYSEAAAMYEKVYRKDQGRTYPDAL
                     RWLGEMQLCAGKYDEARKTWLKVKQKARDKQGTTAIRADNALAGIALAKEWMAEPEDV
                     TIEHLPEPVNSYDSEFGARTGPDGKLYLTSLRGAINADDEVIDSAQYVARIFHSDTTA
                     GAWMVPQATDSIQPGVSMANPAWTPTGLYLASRCDPRRPCRIVIGRGVEERSLTGLGE
                     AQSTQPMVANFQGKETLFFTSDRVGGAGGLDIWLADFDGDRGSIVRPLGPPINTPGNE
                     SCPYYDADQGKLYFSSDFHPGSGGFDNFMSRDSAGTFTAP"
BASE COUNT          399 a          650 c          617 g          319 t
ORIGIN      
        1 accgccagca gtggcgcagc gtcacggttc cgtaccgaac cttcgggatc ggtggcgaca
       61 tggccgacgt tgcgggcttg aagggactcg gcgcgggact ccggctttac aacgaccgtg
      121 cgggagattc ccggctgaac accttccacc tcagtgcggc cgtttcgtgg acccagcggt
      181 tcggcgacca ccatgcgcag agcatcaccg gcggcattca ggcgggcttc acctcgctga
      241 gcatcgacta cagcgcgctc cgctacgatg cgcaatacaa cggctactac ttcgaccctt
      301 cgctcagcaa caacgagcag ttcacacgcg atgcggccac ccatccggac ctgcacgccg
      361 gcctcatcta ccggtacgca ccggacaaaa ggcgatcgat ccaggccggg atcgccctgt
      421 tcaacctcac cacgccgcgc atcgacctca acggcgacct ttcgccactg gaccgcagga
      481 cggccctgca cgtgacaaca cgcttcccgg tgagcgccaa gctcgatgtg caaccgatgg
      541 cgcaatacat ggcacaggcg acattccgtg aactgctgct gggcggaagc gttcgataca
      601 tcctcaagga caagtacacc tacctgcgca ccgtgcaggc cgggctgttc tggcgcgcgg
      661 ccgatgcggg catggtcttc gccggactcg aaatcgacga ctggaccgct ggcctgagtt
      721 acgacctgaa cttcagcaag ctggtgcctg cgagccgcaa ccggggcggc ttcgagatca
      781 ccgtcatccg tgtgttccgg aaaaaaccgt tcgttcccgt gcgctacaag gcatgtccca
      841 cgcaactatg atcgcgccgc ggatgcgcca acgcaccagg accggcgtcc gcggcccgct
      901 ggtcatgctc gtctgcctcc tcgtgcggtc gtcgtgtgcc cagaatgccg cgcagttcgt
      961 cacctggggc gacaacgcga tggcgatcga ggattactac ggggcctcga aattctacgc
     1021 gcaggccatg gaaatggaag gcggacgttt ggaattgcaa tggaaatacg ccgaggccgg
     1081 ccgcctgagc aaccagtact cggaggccgc tgcgatgtac gagaaggtgt accgcaagga
     1141 ccaggggcgc acctaccccg atgcgttgcg ctggctgggt gagatgcaat tgtgcgccgg
     1201 gaaatacgac gaggcccgga agacctggct gaaagtgaag cagaaggccc gcgacaagca
     1261 gggcaccacc gcgatacgcg ccgacaacgc gctcgccggc atcgcgctgg ccaaggaatg
     1321 gatggcggaa ccggaggacg tgaccattga gcacctgccc gaaccggtga acagctacga
     1381 cagcgagttc ggtgcgcgga ccggtcccga tggcaaattg tacctgacca gcttgcgcgg
     1441 cgcgatcaac gccgatgacg aggtgatcga cagtgcgcag tacgtggctc gcatcttcca
     1501 cagcgacacc accgcggggg catggatggt accgcaggcc accgacagca ttcaacccgg
     1561 cgtctccatg gccaatccgg cctggactcc caccgggctc tacctcgctt cgcgatgcga
     1621 cccgcgtcgc ccgtgccgca tcgtcatcgg ccggggtgtt gaagaacgct cgttgactgg
     1681 tctcggcgaa gcacagagca cccaacccat ggtggcgaac ttccagggta aggaaacgct
     1741 gttcttcacc agcgatcgcg taggcggtgc tggcgggctt gacatctggc tggccgactt
     1801 cgacggtgac agggggagca tcgtccggcc gttgggtccg cccatcaaca cgccgggcaa
     1861 cgagagctgc ccgtactacg atgccgacca gggaaaattg tacttcagca gcgacttcca
     1921 cccgggatcc ggcggcttcg acaacttcat gagtcgggac agtgcaggga ccttcaccgc
     1981 accgg
//
LOCUS       DAOQOC010000580         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38599_length_1983_cov_4.027490, whole genome shotgun sequence.
ACCESSION   DAOQOC010000580 DAOQOC010000000
VERSION     DAOQOC010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38599_length_1983_cov_4.027490"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1658)
                     /locus_tag="PL106_09535"
     CDS             complement(81..1658)
                     /locus_tag="PL106_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33352.1"
                     /translation="MLRNALLVALLPPLASAAQAPTWADDVACIVYSHCVSCHRVEGV
                     AGEHLDLTTYQEAHGHRDDIALYTGSRLMPPWPPDENYRSFAHERVLTQDEIDIIAAW
                     ADADGPQGNMANAPTLPAFSGAWEIADPDVSTRMADYDIPVSATDLYRCFVLPYNDAQ
                     AHFIKRLEVVPGNREAVHHVLVFQDTTGEAQGLDAADPGPGYTNFGGIGVNNAPLIGI
                     WAPGSPALNVPDGMGIRMYPGADLVIQVHYPADDVPRLDSTRINLEFAEGQGIREIGI
                     APALDHLYTITDGPLIIPPNEVRTFHAQFTVPFPTTITAIGPHAHLICTSMKAFAVSP
                     ENDTIPLVDIPAWDFHWQGMYQFRQPILLPTGTVLHGEATYDNTPANENNPNDPPAWV
                     TLGEATTNEMMLFYFAYTVPGFTTDTLIVVDDTPHTPHVENCVPDFNIGVNDNKTMAD
                     IRLVPVPATDRLKVTTGLNGAWLRLIDVQGREVATTRLNAGENNVDVSYLARGTLIAE
                     VRDAQGMPVHRGTLILQ"
     gene            complement(1734..>1983)
                     /locus_tag="PL106_09540"
     CDS             complement(1734..>1983)
                     /locus_tag="PL106_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33353.1"
                     /translation="DHLKLILQVPGKDGGLDAIAFRQGKHLDLVRNGTFSLLYTLEEN
                     EWNGRRSLQLNVKDIKPGVGDPHGHGAARSEGPAFAAI"
BASE COUNT          325 a          585 c          669 g          404 t
ORIGIN      
        1 gggcacctcg tccgccgcga acgcacgctc ggcctcgcgc aaggccatgc gcatgaagtg
       61 cgcgtcatcc accgcaccga tcattgcagg atcaaggtac cgcggtgaac gggcatgcct
      121 tgcgcgtcgc gtacttcggc gatgagggta ccgcgtgcca ggtacgatac gtccacgttg
      181 ttctccccgg cgttgaggcg ggtggtggcc acttcgcggc cctgcacatc gatcagccgg
      241 agccatgccc cgttcagtcc ggtggttact ttcagccggt ccgttgccgg caccgggacc
      301 agccgtatgt cggccattgt cttgttgtca ttcacaccga tgttgaagtc cggcacgcaa
      361 ttctccacgt gcggcgtgtg cggggtatcg tccaccacga tgagcgtgtc ggtggtgaag
      421 ccgggcacgg tgtacgcgaa gtagaagagc atcatctcgt tggtggtggc ctcacccagc
      481 gtgacccatg ccggaggatc gttcgggttg ttctcgttgg ccggagtgtt gtcgtacgtg
      541 gcttcgccat ggagcacggt gccggtcggc agcaggatgg gctggcggaa ctggtacatg
      601 ccctgccagt ggaagtccca ggcggggata tccaccagcg ggatggtatc gttctcgggc
      661 gacacggcga acgccttcat gctggtgcag atcaggtgcg cgtgcgggcc gatggcggtg
      721 atggtggtgg ggaagggcac ggtgaactgc gcgtggaagg tgcgcacctc gttgggcggt
      781 atgatgagcg ggccgtccgt gatggtgtag agatgatcca atgcgggtgc gatgccgatc
      841 tcgcggatac cctggccctc ggcgaactcc aggttgatcc gtgtgctgtc cagccggggc
      901 acgtcgtccg ccgggtaatg tacctggatc accagatcag cgcccgggta catcctgatg
      961 cccatgccgt cgggaacgtt gagcgcgggc gagccgggtg cccagatgcc gatgagtggc
     1021 gcgttgttca ccccgatgcc gccgaagttg gtatagcccg ggcctgggtc agcggcatcc
     1081 agcccctggg cttcgcccgt agtgtcctgg aacaccagca catgatgtac ggcctcccgg
     1141 ttcccaggta ccacttcgag ccgcttgatg aagtgcgcct gcgcgtcgtt gtacggcagc
     1201 acgaagcagc ggtacaggtc agtggcggac accggtatgt cgtaatcggc catgcgcgtg
     1261 ctgacatcgg gatcggcgat ctcccaggcg ccgctgaacg cgggtaaggt gggtgcgtta
     1321 gccatgttgc cctgcggtcc atcggcatcg gcccatgcgg caatgatgtc gatctcgtcc
     1381 tgcgtgagca cccgttcgtg cgcgaagctc cggtagttct cgtccggtgg ccaggggggc
     1441 atcagccggg atccggtgta gagcgcgatg tcgtcgcgat gaccatgtgc ctcctggtac
     1501 gtggtgaggt ccaagtgctc cccggcgaca ccctccacgc ggtggcagct gacacaatgg
     1561 ctgtacacga tgcaagccac gtcgtcggcc cacgtgggtg cctgcgcagc ggatgccagc
     1621 ggtggcaata gtgcaacaag aagggcgttc cgtaacatta ctggtggatc gttgtgtggt
     1681 gaaaagtagg acaaggcagg agccgatcgg ttcgatcgcc ctatgatctt tcttcaaatg
     1741 gcggcgaatg ccggtccctc actcctggcc gcaccatgcc cgtgcggatc acccacgccc
     1801 ggtttgatat ccttcacgtt caattggagc gatcggcggc cgttccattc gttctcttcc
     1861 agtgtgtaca agaggctgaa cgttccgttg cgcaccaggt ccaggtgctt gccttggcgg
     1921 aaagcgatcg cgtcgaggcc tccgtccttg cccggcactt gcaggatcaa tttcaggtga
     1981 tcc
//
LOCUS       DAOQOC010000581         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38658_length_1982_cov_2.940841, whole genome shotgun sequence.
ACCESSION   DAOQOC010000581 DAOQOC010000000
VERSION     DAOQOC010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38658_length_1982_cov_2.940841"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      119..252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            318..1592
                     /locus_tag="PL106_09545"
     CDS             318..1592
                     /locus_tag="PL106_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33354.1"
                     /translation="MYRSFLLAPALATALFANAQLYTHQVLVLNEGHYDFNTQTQDVP
                     VTLGSYNPATGTYSTVATIADARFGNAVVVDGHTIYVGADTRLLKYDADTYALLDEAE
                     VPGIRSLAVWNNALVIPRGEVGGLPYYLEVRDKHTLDELYHLDAATLPYSCEAVQVVG
                     DQAFIAVNNGFDWGNAVGLLGVLDMNSQTWSTLDLGPDGINPEHIMVVDGAVYTFNNK
                     DYTGSSISKYQPGGVAVDYTHNVALSSGCGASAATTDRIYFLEYAQNVLNRYDLATAA
                     VLDTLDASPATYGLIDDPNNGVMYGTTTDFLTSGELHVMNYEGQILSSVAVGVSPGKL
                     ALDTRLSTGITTGTTVRPVLYPNPATDMLSVSLSAAHGNVPFTLHDAAGRTVLQGAMN
                     SSGTLRIDVSDLAAGVYTLHTVNGATARFIKR"
     gene            1687..1758
                     /locus_tag="PL106_09550"
     tRNA            1687..1758
                     /locus_tag="PL106_09550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1719..1721,aa:Thr,seq:cgt)
BASE COUNT          401 a          647 c          577 g          357 t
ORIGIN      
        1 ctcatccgga tcgtgttcgc ggccaagtta tcaagcggtg tacaccggcg tggagttcgt
       61 ttccgggctg atccgatctc ctccaaaagt gctggctaca ttcgccccgc gtccgtccaa
      121 gggaatcccg tgcgatccgg gaactgttcc cgcagctgtg aaccatcgga acgcccagcg
      181 ctgaagccac tgtacccgca gatgcggttg tcgggaaggc gcgccgggaa aaggtggtga
      241 gtcagaagac ctgaaacggc cgcaggtcac ttccatacaa ccgccttccg gtgaagaagg
      301 cacaaaaacg tttccgcatg taccgttcat tcctcctggc cccggcgctg gccaccgcgc
      361 ttttcgcgaa cgcccaactg tacacccatc aggtgctcgt cctgaacgag ggtcactacg
      421 atttcaacac ccaaacgcag gatgtgcccg tcacccttgg cagctacaac cccgccacgg
      481 ggacatacag cacggtggcc accatcgccg atgcgcgttt cggcaatgcc gttgtggtgg
      541 acggtcatac gatctatgtg ggggctgata cccgactgct gaagtacgat gccgacacgt
      601 acgcgctgct cgatgaggcg gaggtgcccg gcatccgcag cctggccgtg tggaacaatg
      661 cgctggtgat cccccgcggc gaagtgggcg gcctgccata ctacttggaa gtgcgcgaca
      721 agcacacgct ggacgaactg taccacctgg acgccgcgac cctcccgtac agctgcgaag
      781 ccgttcaagt ggtcggcgac caggccttca tcgccgtgaa taacggattc gattggggca
      841 atgccgttgg ccttctcggg gttctcgaca tgaacagcca gacctggagc accctggacc
      901 tgggacccga tggcatcaac cccgagcata tcatggtggt ggatggcgcc gtgtacacct
      961 tcaacaacaa agactacacc ggaagcagta taagcaaata ccagcccggc ggtgtggcgg
     1021 tggactacac gcacaatgtg gcgctcagct ccgggtgcgg cgcctcggcg gctaccaccg
     1081 accgtatcta cttcctggaa tacgcgcaga acgtgctgaa ccggtacgac ctggccaccg
     1141 ccgccgtcct ggacacgctc gacgcatcgc ctgccacgta cggcctgatc gacgacccca
     1201 acaacggggt gatgtacggt accaccaccg atttcctcac atccggcgag ttgcacgtga
     1261 tgaactacga gggccagatc ctcagcagcg tagcggtggg ggtgagcccc ggtaaactgg
     1321 cgctcgacac gcgtttgagc accggcatca caaccgggac gacggttcgt cccgtgctgt
     1381 accccaaccc ggccacggac atgctttcgg tgagcctttc ggcggcccat ggcaacgtac
     1441 cgttcacgct gcacgacgcg gcgggccgca cggtcctcca aggtgccatg aacagttccg
     1501 gtacgctccg gatcgatgtt tccgacctgg ccgccggcgt gtacaccttg cacacggtca
     1561 acggtgcaac agcgcgcttc atcaagcgtt gaagagatcg aggtgacgag tgggggcggt
     1621 cctctacatt tgccgccctt ccgtcctgtt atcctgagcc cgccgaggga tgacaggacg
     1681 gcttcagcct ccctagctca gcggtagagc agctcattcg taatgagcag gtcgccggtt
     1741 caaatccggc ggggggctcc gcaagaaggc cactgtcaac cgacggtggc cttcgctatt
     1801 gggtgtacct tcagtgctgt tgtgactgat gatcgccgac agcgtcccaa acacgcatca
     1861 aacacctgac caccatgctc gacgagatcc tttccaccct gaaaagccag gccgcgcctg
     1921 agctcatgaa caccatcggc ctgaacgcac agttgctcac cgagggcgtc gcccacccct
     1981 gc
//
LOCUS       DAOQOC010000582         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38661_length_1982_cov_2.901920, whole genome shotgun sequence.
ACCESSION   DAOQOC010000582 DAOQOC010000000
VERSION     DAOQOC010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38661_length_1982_cov_2.901920"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..99)
                     /locus_tag="PL106_09555"
     tRNA            complement(26..99)
                     /locus_tag="PL106_09555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Ala,seq:ggc)
     gene            201..1097
                     /locus_tag="PL106_09560"
     CDS             201..1097
                     /locus_tag="PL106_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNA33355.1"
                     /translation="MPDSLLHTLAHRLSKHLGRTVTVDRETPVGGGSISDSYLLDTTA
                     GRYFVKVDPAPRTATGAPHFPSMFAAEADGLERLASTRAIRIPKVIATGDEDFGYLLL
                     ERIECRPTTSAFWTVFGHALARLHGNAAPHFGLDRDNWIGPLKQLNARTSDWTTFFIQ
                     QRLEPQVKSARDNHRLGMGEVLRFERLYMKLPVLIPTEPPALLHGDLWHGNFLCDERE
                     QPVLIDPAVYFGHREMDIAMSRLFGGFDASFYEGYNAERPLERGWEERMDLHNLYPLL
                     VHLNLFEGGYAQQVRAVLGRYV"
     gene            complement(1171..1713)
                     /locus_tag="PL106_09565"
     CDS             complement(1171..1713)
                     /locus_tag="PL106_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33356.1"
                     /translation="MQVQLVEDTIHLMDAGGGIRTLPMMSREAMSAVVLVRPSMDVDV
                     LTAPLKYRLATGNARPRLETQLGGLIYFGRRMDRYRLTWPDLGFASRARKEDHIGLSM
                     GLFVGLGGVQVAPWTTGNRLEEDYTGVAASAGCALIGAVGSTTLGAAIGWDHLLNDQH
                     RVWIYEGRPWLGLVFGVNLN"
     gene            complement(1812..>1982)
                     /locus_tag="PL106_09570"
     CDS             complement(1812..>1982)
                     /locus_tag="PL106_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNA33357.1"
                     /translation="VATVVFMPPTLVASIYGMNFEHMPELAPTWGYPFALALMLLSSG
                     FTLHIFKRKGWL"
BASE COUNT          387 a          647 c          586 g          362 t
ORIGIN      
        1 cgcttccgag gaagcggcct tcgtgtggag aataccggag tcgaaccggt gacctcttgc
       61 atgccatgca agcgctctgg ccagctgagc taattcccca gaatgtcaac cgtttccgtt
      121 cgggccgcac cagccttttc ccgaacgggg tgcgaattta gcacgatccc agcgaacacg
      181 acacctttgc gtgcatgccc ttgcccgaca gcctcctcca cacgctggcc caccgactga
      241 gcaagcatct tggcaggacc gtgaccgtgg atcgggaaac gcccgtgggc gggggctcca
      301 tcagcgatag ctacctgttg gacaccacgg ccgggcggta cttcgtgaag gtggatcccg
      361 ccccacgtac cgccaccggg gcaccgcact tcccttccat gttcgcggct gaggccgatg
      421 gactcgaacg gcttgcatcc acccgcgcca tccgcattcc gaaggtgatc gccacgggcg
      481 atgaggactt cggttacctc ttgctggaac gcatcgaatg ccgtccgacg acctcggcct
      541 tctggacggt tttcgggcac gccctggccc ggctgcacgg gaacgcagcc ccccacttcg
      601 gtctggatcg cgacaattgg atcggcccgc tcaagcagct gaacgcccga acatcggatt
      661 ggaccacctt cttcatccaa caacggctgg aaccccaggt gaagtcagcg cgagacaacc
      721 atcgtctggg catgggcgag gtgttgcgtt tcgaacggtt gtacatgaag cttcctgtcc
      781 tgatcccgac cgagccaccg gcgttgctgc acggcgacct ctggcacggg aacttcctgt
      841 gcgatgaacg ggaacaaccc gtgctgatcg accccgctgt gtatttcggc caccgcgaga
      901 tggacatcgc catgtcccgg ctcttcggcg gtttcgacgc ttcgttctat gagggataca
      961 acgccgagcg gccgctggaa cggggatggg aggaacgaat ggacctgcac aatttgtacc
     1021 cgttgctggt gcatctgaac ctgttcgagg gagggtacgc ccagcaggta cgggcggtgt
     1081 tggggcggta cgtgtgagcg acccttcccg agcgggaggg tcaccgatcc gatccccgct
     1141 cctacgaccg gctatctgaa tggaccggat tcagttcaag ttgacgccga agaccaagcc
     1201 gagccagggc cgcccttcat agatccacac ccggtgttga tcgttcaaca ggtggtccca
     1261 gccgatggct gcgccgagcg ttgtactgcc cacggcaccg atgagggcgc aaccggcact
     1321 ggcggccaca ccggtgtaat cctcttccag gcgattgccc gtggtccacg gagccacctg
     1381 taccccgccc aacccgacga aaaggcccat gctcaaaccg atgtgatcct ccttgcgagc
     1441 gcgtgaggcg aaacccaggt cgggccaggt gagtcgatac cgatccatgc ggcgaccgaa
     1501 gtagatgagg ccacccaatt gcgtttccag ccggggtctt gcgttaccag tggccaagcg
     1561 gtacttcagc ggtgcggtga gcacatccac atccatgctc ggccgtacca gtaccaccgc
     1621 tgacatcgct tcgcggctca tcatcgggag cgtgcgtatg ccaccaccgg catccataag
     1681 gtgaatggtg tcctccacca gctgtacctg cacgggttta ccttccgatc ggtatcgtcc
     1741 atcggccaat ccacgcttcg ctccgccaca agcgcagcaa agccacgcga tcagcacggc
     1801 cgggagccac ctcacaacca tcccttccgt ttgaaaatat gcaaggtgaa cccggaactg
     1861 agcaacatga gggccagggc gaacgggtat ccccaggtgg gcgccagttc gggcatgtgc
     1921 tcgaagttca tcccatagat gctggcgacc agggtgggcg gcatgaagac caccgtggcc
     1981 ac
//
LOCUS       DAOQOC010000583         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38733_length_1980_cov_3.168831, whole genome shotgun sequence.
ACCESSION   DAOQOC010000583 DAOQOC010000000
VERSION     DAOQOC010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38733_length_1980_cov_3.168831"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PL106_09575"
     CDS             <1..584
                     /locus_tag="PL106_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNA33358.1"
                     /translation="PVATKLYWRVVLGWFFRGLLLTVPIAVIGWAAVKALNLLDSLIP
                     METPGLGLLILVGSVTFIGWLGSTVLYEPVAEFGAEVLKRIPLLKTVYDALSDMMEAL
                     VGSKRKFDQPVLVRLTPGPVPEKVGFMTSKDLGHLGIPGNKVAVYLPHSFAWSGDLYI
                     VPAENVTLLDARASDVMKFIVSGGVVEADEPRT"
     gene            630..1493
                     /locus_tag="PL106_09580"
     CDS             630..1493
                     /locus_tag="PL106_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33359.1"
                     /translation="MKVLLTALAALTASLSFGQTQVTNASFKYSDAVHPTFSVTIPGT
                     SADKAEKWFKEQLKSISASIGGKKEVIGIGTRLPEVSSDTIRVFVVADQPKKSDEVTV
                     HAAFRVNMAFVGTDGDEAQREGCRKWVYQTAVGLKKLLAQEDLDAANKILATLEGDLA
                     GLVKEKGRAEGNLEKTQRSIVDDQKSKVEAEGESKILETKVESKKQEVVSAPSEANTK
                     ELQALMKEQEKLHKKADKLTESIAAGKRKVEDLQFQIKKNLSEQDTKTKAIATQKDAV
                     ALLTAKLAGIN"
     gene            1548..>1980
                     /locus_tag="PL106_09585"
     CDS             1548..>1980
                     /locus_tag="PL106_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA33360.1"
                     /translation="MTPAYRNALLLLHLTVFLWGWTAIFGKLITQDALQLVFTRTFIA
                     VCGIGVFILVSGRSLLSGIGDIRVCLLTGVLIWAHWFTFYLSIKVSTASIAAACMATS
                     TLFTALMEPLWFKRRVRPYEIILGLVVVGALLLLFGLETRYR"
BASE COUNT          412 a          595 c          629 g          344 t
ORIGIN      
        1 gccccgtggc aacgaaactt tactggcgcg tcgtccttgg ctggttcttt cgggggctcc
       61 tgctcaccgt gcccatcgcg gtgatcggtt gggcggcggt gaaggcgctg aacctgctcg
      121 acagcctgat ccccatggaa acacccgggc tgggcctgct catcctcgtg ggcagcgtca
      181 ccttcatcgg atggttgggc agcaccgtgc tgtacgaacc cgttgccgaa ttcggtgccg
      241 aggtgctcaa gcgcattccc ctgctgaaga ccgtgtacga cgccctgtcg gatatgatgg
      301 aggccctggt gggcagcaaa cgcaagttcg atcagcccgt attggtgcgg cttacccctg
      361 gccctgttcc ggagaaggtc ggattcatga cctccaagga cctggggcac ttgggcattc
      421 caggcaacaa agtggccgtg tacctgcccc acagcttcgc atggagcggc gatctttaca
      481 tcgtgccggc ggaaaatgtg accctcctcg atgccagggc cagcgacgtg atgaagttca
      541 tcgtcagcgg cggcgtggtg gaagcggacg aaccccgcac gtgaccgatc ggcacgtcaa
      601 ttgacacgca caacgaaaac agccccttga tgaaagtcct ccttaccgcg ctcgcagcgc
      661 tcaccgcatc gctctccttc ggtcagaccc aagtgaccaa tgcatccttc aagtacagcg
      721 atgcggtgca tcccacgttc agcgttacca tccccggcac cagcgccgac aaggcggaga
      781 agtggttcaa ggaacaactc aagagcatca gcgcgagcat cggtggcaag aaggaggtga
      841 tcggcatcgg cacgcgcctg cccgaggtga gcagcgacac catccgcgtg ttcgtggtgg
      901 ccgatcagcc caagaagagt gatgaggtga cggtacatgc cgcgttccgc gtgaacatgg
      961 cctttgtggg caccgatggc gatgaggccc aacgcgaagg ctgccgcaag tgggtgtatc
     1021 agaccgctgt gggcctgaag aagctcttgg cccaagagga cctggacgcg gcgaacaaaa
     1081 tactcgccac actcgagggt gatctggccg gactggtgaa ggagaaaggg cgtgccgagg
     1141 gcaacttgga gaagacgcag cggagcatcg tggacgacca gaagagcaag gtggaggccg
     1201 agggcgagtc gaagatcctg gagaccaagg tggagtcgaa gaagcaggaa gtggtgagcg
     1261 ccccgtccga agcgaacacc aaggaactgc aagcgctgat gaaagaacag gagaaactgc
     1321 acaagaaggc cgacaaactc accgagagca tagccgcagg caaacgcaag gtggaggacc
     1381 tgcagttcca gatcaaaaag aacctgagcg agcaggacac caagaccaag gccatcgcca
     1441 cccagaagga tgccgttgcg ctgctcaccg cgaagcttgc cgggatcaat tgaccgggtg
     1501 ccgatgggcg gtccgaaggc ggcgggcgcc ccggctatct tcaccgcatg acccccgctt
     1561 accggaacgc cctgctgctg ctccacctca cggtcttcct ctggggttgg acggccatct
     1621 tcggcaagct catcacacag gatgcgctcc aactcgtgtt cacgcggacc ttcatcgcgg
     1681 tctgcggcat cggggtgttc atcctggtct cggggcgatc actgctcagc ggtatcggtg
     1741 acatccgtgt gtgcctgctc acgggcgtgc tcatctgggc gcactggttc accttctacc
     1801 tcagcatcaa ggtgagcacg gccagcatcg cggcggcgtg catggccacg agcaccctct
     1861 tcacggcact gatggagccc ctctggttca aacgccgtgt gcggccgtac gagatcatcc
     1921 tgggcctggt ggtcgtcggg gcgctattgc tgctgttcgg gttggagacc cggtaccggc
//
LOCUS       DAOQOC010000584         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38956_length_1974_cov_3.660240, whole genome shotgun sequence.
ACCESSION   DAOQOC010000584 DAOQOC010000000
VERSION     DAOQOC010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38956_length_1974_cov_3.660240"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="PL106_09590"
     CDS             complement(<1..1700)
                     /locus_tag="PL106_09590"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNA33361.1"
                     /translation="MDFKKLNIATGWLVFLVATWTYISTIEPTASFWDCGEFLATANK
                     LQVGHPPGAPLFMMLGRVASAFVSTENVPVAINILSALCSSFTILFLFWSITHMAKKL
                     ATRDTDAPLSTGQLIAVLGSGIVGSLAYTWSDSFWFSAVEAEVYSMSSFFTAITFWAI
                     LKWESEAHEPHNTRWLILICYLLGLSIGVQLLGLLCIPAIGLVYYFKKYKTTTQGVIW
                     TMEISAVILGTIQSIIIPGVAKVAGKFELLFVNGMGLPFNSGNLVYGALLVSLTVWGL
                     LWSQRNGKVIINTIILGVAVILLGYSTYAMTVVRSLANPPIDENNPENVFNLVSYLNR
                     EQYGDRPLLIGQFWDSELSEQRGNGTPVYTATYQVLKNGRPEKVFYDGWSAEHYVAGK
                     PDLTVDHSYVITDKREGTEPEYEPQFTMLFPRMYSSQPSHVTQYKDWCDFKGVPIRWT
                     GRDGKPTIIQKPTQAENLRFFMSYQVNHMYWRYFMWNFAGRQNDIQSTGSSILDGNWY
                     TGLKFVDEARLGDQDHLPPSMTWNKGMNKFYLLPLLLGIIGLVYQLSRNVRDWSIVAL
                     L"
     gene            1863..1935
                     /locus_tag="PL106_09595"
     tRNA            1863..1935
                     /locus_tag="PL106_09595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Gln,seq:ttg)
BASE COUNT          373 a          540 c          659 g          402 t
ORIGIN      
        1 aggagcgcca cgatggacca gtcgcgcaca ttgcgggaca gctggtacac caggccgatg
       61 atcccgagca gcaagggcag gaggtagaat ttgttcatcc ccttgttcca cgtcatgctc
      121 gggggcaggt ggtcctggtc gcccaagcgg gcctcgtcca cgaacttcag gccggtgtac
      181 cagttgccat caagaatgct gcttcccgtg gactggatgt cgttctgcct gccggcgaaa
      241 ttccacatga agtaccgcca gtacatgtgg ttcacctggt agctcatgaa gaaccggagg
      301 ttctcggcct gggtgggctt ctggatgatg gtgggcttgc catcgcggcc tgtccagcgg
      361 atgggcacgc ccttgaagtc gcaccagtcc ttgtattgcg tcacatggct gggctggctg
      421 ctgtacatgc gcgggaagag catggtgaac tggggctcgt actcaggttc ggtaccttcc
      481 cgtttatcgg taatgacata ggagtggtcc accgtcaggt ccggcttgcc cgccacatag
      541 tgctccgcgc tccagccgtc gtagaagacc ttttcggggc ggccgttctt gagcacctgg
      601 taggtggcgg tgtacacggg ggtgccgttg ccgcgttgtt cggacaattc gctgtcccag
      661 aattgtccga tgagcagcgg gcgatcgccg tactgctcac ggttcaggta gctcaccagg
      721 ttgaacacgt tctccgggtt gttctcgtcg atgggcgggt tggccaggct gcgcaccacg
      781 gtcatggcat aggtgctgta gcccagcagg atcacggcca cgcccagtat tatggtattg
      841 atgatcacct tgccgttccg ttgcgaccac agcagccccc acacggtgag gcttacgagg
      901 agtgccccgt ataccaggtt ccccgagttg aaggggaggc ccatgccgtt cacgaagagc
      961 agttcgaact tgccggccac cttggccaca ccggggatga tgatgctctg gatggtgccc
     1021 aggatgaccg cgctgatctc catcgtccag atgacgccct gcgtggtggt cttgtacttt
     1081 ttgaagtagt acaccagtcc gatggcgggg atgcagagca ggcccaggag ctgcacgccg
     1141 atgctgaggc ccagcaggta acagatcagg atgagccagc gggtgttgtg cggttcatgc
     1201 gcttcgctct cccatttgag gatggcccag aaggtgatgg ccgtgaagaa ggacgacatg
     1261 gaatacacct cggcctccac cgcactgaac cagaagctgt cgctccaggt gtaggccagc
     1321 gagcccacga taccgctgcc gagcacggcg atgagctggc cggtggagag gggagcgtcc
     1381 gtgtcgcggg tggcgagttt cttcgccatg tgcgtgatgc tccagaaaag gaagaggatg
     1441 gtgaaggaac tgcacagcgc actgaggatg ttgatggcca cgggcacgtt ctccgtggac
     1501 acgaaggcgc tggccactcg gccgagcatc atgaagaggg gggccccggg ggggtggccc
     1561 acctgcagtt tgttggctgt ggccaggaac tcgccgcagt cccagaagct ggcggtgggc
     1621 tcaatggtgc ttatgtaggt ccacgtcgct acgaggaaga cgagccaacc cgtggcgatg
     1681 ttcaacttct tgaagtccat gcggtaagga gggggaaaag ccgccgctgg ggcggggcgg
     1741 gcgaaagtaa cctgaagtac cggagaccaa agccaaggaa cgtgagcgcg tatcgttgat
     1801 ctgtttactt ttgcgccgct ttcgcggaag ggatacttcc ggcggaagcc aggtacactt
     1861 gatggccgat ggtgtaactg gcaacacgtc tggttttggt tcagaagagt ccaggttcga
     1921 gccctggtcg gccaactctg agtccgaggt cccgctgtga ggcgggacct cgcg
//
LOCUS       DAOQOC010000585         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_38991_length_1973_cov_4.022941, whole genome shotgun sequence.
ACCESSION   DAOQOC010000585 DAOQOC010000000
VERSION     DAOQOC010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38991_length_1973_cov_4.022941"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PL106_09600"
     CDS             <1..378
                     /locus_tag="PL106_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33362.1"
                     /translation="PGGDCGWDPEGLEMPVEAISSFALPWTFNPRVLTTGIADQGTIG
                     SQLMISPNPTADLVTLGWPMNMEPPLALMILDASGRVVHHLDRLERTGSLTLNVSDLA
                     EGHYTACLRTGNGLLRRPLVIAR"
     gene            complement(515..1216)
                     /locus_tag="PL106_09605"
     CDS             complement(515..1216)
                     /locus_tag="PL106_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNA33363.1"
                     /translation="MPNTPRQAVLPDAEPAIRAHSTIMDTAGMAADLRIIERKLGPLD
                     PAVRGELLPLEVEYFTFSDSTCGTVDTTRTWTGILIVHSCIAEDVRAVFRAMLADTFP
                     IAKVIPINRYGLNADSTGWNDAASMADNNTSAFNYRGKPTSSRPSKHGLGISIDINPL
                     LNPMLHHRASGVHVQPPQGRYDPARPGTLTRANTGRFLGDLGWAWGGRWPQPQDFQHI
                     EKVRGGCAHMRFSLK"
     gene            complement(1448..>1973)
                     /locus_tag="PL106_09610"
     CDS             complement(1448..>1973)
                     /locus_tag="PL106_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33364.1"
                     /translation="DATTGLATPIPDTVSMVHAGHGQWDRINYFGIHGYEFGFEGEHR
                     KRLVCEKERVNGIAEKVTSTIDFIEPGMIGKFQGATKDMEPITYQNGPFILSKTKAGN
                     RFAWRIDLMDTRTLDSKWSVELDLTRPDGRDNEIMDLALNEHRLTILAKNFIGALDAR
                     TGAWVWQHNFVSTE"
BASE COUNT          358 a          601 c          587 g          427 t
ORIGIN      
        1 ccgggtggcg attgcggctg ggatccggaa ggtcttgaaa tgcctgtgga agccatctcg
       61 tcctttgcac ttccttggac cttcaacccg agagtgctca ccaccggaat tgcggatcag
      121 ggaacgatcg ggtcccagtt gatgatcagt ccgaatccga ccgccgacct ggtgaccctg
      181 ggttggccta tgaatatgga gccgccgctg gcgctgatga tcctggatgc ttctggccgc
      241 gtggtccatc atcttgatcg gttggaacgc acgggatcct tgacgttgaa cgtgtcggac
      301 ctggccgaag gacactacac ggcatgcctt cgaacgggca acggcctcct gcgcaggcca
      361 ctggtgatcg cacgttgatc gcgaacgcat gaacgatagc cccgaccggg atcaaggtcg
      421 gggttgtccg tatccggacc agggaactcc gtgatcacgt gttccgggga tccatcaccg
      481 ctccctgctg catagacagg tccgggcaca acctctattt caggctgaag cgcatgtgcg
      541 cacacccgcc cctcaccttc tcgatatgct gaaagtcctg aggttgcggc cagcggcctc
      601 cccacgccca accgagatcg ccaaggaatc gcccggtatt cgctcgcgtg agcgtacccg
      661 gccgggctgg gtcgtatctc ccttgaggcg gctgcacatg aacccccgac gcacggtgat
      721 gcagcatcgg gttcaacagg ggattgatat cgatggaaat tccaagacca tgcttggacg
      781 gcctgctgct ggtcggttta ccgcggtagt tgaacgccga ggtgttgttg tcggccatgg
      841 aggccgcatc gttccatccg gtgctgtccg cattcaggcc atacctgttg atggggatca
      901 ccttggcgat cgggaaggtg tccgcgagca tggcgcggaa gacggcccgt acgtcctcgg
      961 cgatgcagct atggacgatg aggatgccgg tccaagtgcg cgtggtgtcc actgtgccac
     1021 atgtgctgtc cgagaacgtg aagtactcca cctccagtgg gagcagttca cctcgaacag
     1081 cgggatccag ggggccgagc ttgcgttcga tgatccgcag gtccgcggcc atacccgccg
     1141 tgtccatgat cgtgctgtgc gcacggatcg caggctccgc atcgggcagg acggcctgcc
     1201 gtggggtgtt cggcatgtgg tcggtggatg aagtgcgctc cggaccggcg gtcgaacagc
     1261 ctgcaccgca catcagtaac aggaccgcgg ccattgaggg ccaggcccat gtgcgtagaa
     1321 cgcagagcca agtgtgccct accccaccct tccatgcttg tccctgttca gcagtcctgc
     1381 tcattcgtca ggttctctcc cagtacgaaa gctgatcagg ccttcacgcc tatgggttgg
     1441 ccatccgtca ctcggtgctg acaaagttgt gctgccagac ccatgcaccg gtccgcgcat
     1501 ccagtgctcc aatgaaattc ttggcgagta tggtgagccg atgctcgttg agggccagat
     1561 ccatgatctc gttgtcccga ccatcgggac gcgtcaggtc caattccacc gaccacttcg
     1621 agtccagggt ccttgtatcc atcaggtcga tccgccatgc gaatcggttg ccggccttgg
     1681 tctttgataa gatgaatggt ccgttctggt aggttatcgg ctccatgtcc ttggtggccc
     1741 cctggaactt gccgatcatc ccaggctcga tgaagtctat cgtggacgtc accttttccg
     1801 cgatgccgtt gacccgctcc ttttcgcaca ccaaccgttt gcggtgctca ccctcgaaac
     1861 cgaattcata tccatggatc ccgaagtagt tgatccgatc ccattgtcca tgtcccgcat
     1921 gcaccatgct aacggtgtcc ggaatgggcg tggccaaacc tgttgtggca tcg
//
LOCUS       DAOQOC010000586         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39011_length_1973_cov_2.776851, whole genome shotgun sequence.
ACCESSION   DAOQOC010000586 DAOQOC010000000
VERSION     DAOQOC010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39011_length_1973_cov_2.776851"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..500
                     /locus_tag="PL106_09615"
     CDS             357..500
                     /locus_tag="PL106_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33365.1"
                     /translation="MKLDDGFQEQQPNETSYAQFMSMLLDAIAAVQAGQKATTEQQQA
                     TNA"
     gene            530..967
                     /locus_tag="PL106_09620"
     CDS             530..967
                     /locus_tag="PL106_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33366.1"
                     /translation="MKRTRLLKPYKEDGSTTFPARQKRGVYLIYRVRLLGEPVLRYVG
                     FSGVDVYKALYRHFQVWNDRQADRGERGERITYLPPGSYRVRVVYTRTKAESVELEQA
                     LILKHRPPDNPDKLELYELTKEGKRLANEAEGAAFVENIEVPF"
     gene            970..1599
                     /locus_tag="PL106_09625"
     CDS             970..1599
                     /locus_tag="PL106_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33367.1"
                     /translation="MNERYRYSDHTKPVDTTPPKVDVVMIQVREDDHRWVKEAVKSVE
                     DQSYPHLGLLIVDNRDRALTIGQAWNGAVQASDADLVLFLGDDDALVHDLVASMVSTW
                     AWMKAKVPNLVHVTTNCTLVDDNSGAVAIGPIHHTGMFLRQFLVEHPFDESLAKGVGK
                     QKMQAIAQAQKFLGQAMSSGIMHHYGYIFRQHPWMVGGQQVRFSANGQR"
     gene            1613..1882
                     /locus_tag="PL106_09630"
     CDS             1613..1882
                     /locus_tag="PL106_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33368.1"
                     /translation="MRGPALAVLIMDLLRSGPSADTAPISMWDLNRNLCDGAALPRGK
                     AREYQYSRVRRMVGTLERAGLLRSEKQWEADNARYIKLLWPCSTR"
BASE COUNT          463 a          590 c          611 g          309 t
ORIGIN      
        1 gacgcctctt cttcctgccc cgctataaac gcttcgtgtg ggagcgtacc gagcgcgtga
       61 gcgatgccaa ggcccgcgcc gaaggcaaca agagcgcttc gagcaaagcg aagcgaccga
      121 gcgaagcgaa gcgctcgaag gccaagacca ggcccaagaa gtgaaaccca cccgacgcga
      181 agagcaggcg cggaccttat gtgcagcctt tcggtggtgg ctctgtgacc gatgacagcc
      241 tggaaagcat agcgatacga caggcacgga cgggtataaa tgcactaaac gggaaagcgc
      301 tctgacagct cgcaaagacg gccgcccgag gggagcgtta tcccctcacc atcgaaatga
      361 agctcgatga tgggttccag gagcaacagc cgaatgaaac gagctatgcc cagttcatga
      421 gcatgctgct ggatgcgatc gctgcagtac aagcaggcca gaaggcgaca accgaacaac
      481 aacaggccac gaacgcttga acgcttcgct tgaagcgacc gaccgaccga tgaagcgcac
      541 ccgcctactg aagccctaca aggaggacgg cagcaccacc ttccccgctc ggcagaagcg
      601 cggggtgtac ctcatctacc gcgtgcggct cctgggtgaa cccgtgctcc gatacgttgg
      661 cttcagtggt gtggacgtat acaaagcgct ctaccggcat ttccaagtgt ggaacgaccg
      721 gcaggccgat cgcggagagc gtggcgaacg catcacctac ttgccaccag gcagctaccg
      781 ggtgcgcgtg gtctataccc gcaccaaggc cgaatccgtg gagctggaac aagcgctcat
      841 cttgaagcac cgcccgcccg acaaccccga caagctcgaa ctctacgagc tgactaagga
      901 gggcaagcgc ctggccaatg aagcggaggg ggccgccttc gtggagaaca tcgaagtgcc
      961 cttttgagca tgaacgaacg ctatcgatac agcgaccata cgaagcccgt ggacactacc
     1021 ccgccgaagg tggacgtggt gatgatacag gtgcgcgaag acgatcaccg ctgggtgaag
     1081 gaggccgtga agagcgtgga ggatcagagc tatccccatt tgggcctgct catcgtggac
     1141 aacagggacc gagccctcac catcggtcaa gcgtggaacg gagcagtgca agcgagcgat
     1201 gccgacctgg tgctcttcct gggcgatgat gatgcccttg tacatgacct ggtggccagc
     1261 atggtgagca cctgggcatg gatgaaggcg aaggtgccca acctggtgca cgttacaacg
     1321 aattgtacgc tggtggacga caacagcgga gccgtggcca tcggccccat ccatcacacc
     1381 ggtatgttcc tgcggcaatt cctggtggag catccgttcg atgaatccct ggccaagggc
     1441 gtgggcaagc agaaaatgca ggccatagca caggcacaga agttcctggg ccaggcaatg
     1501 agttcgggca tcatgcacca ttacggctac atcttcagac aacacccctg gatggtcgga
     1561 ggacaacagg tacgattttc tgccaatggc cagcgctgaa gaacacaacc gcatgcgcgg
     1621 cccagccctg gccgtgctca tcatggacct gctgcgtagt ggccccagcg ccgataccgc
     1681 gccgatcagc atgtgggacc tgaaccgcaa cctgtgcgat ggtgctgcgt tgccgcgtgg
     1741 caaggcccgc gaataccaat acagccgggt gcgccgcatg gtgggcacat tggagcgggc
     1801 cgggctgttg cgctccgaaa agcagtggga agccgacaac gcccgctaca tcaaactgct
     1861 ctggccatgt tcgacgcgct gaagggcatg ggcgacctgt ccaaggtcat cggtgcggcc
     1921 atgcggggcg acaagagcgg catcatcgcc atgctcaagc ccgaactgcc cgg
//
LOCUS       DAOQOC010000587         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39073_length_1971_cov_3.245303, whole genome shotgun sequence.
ACCESSION   DAOQOC010000587 DAOQOC010000000
VERSION     DAOQOC010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39073_length_1971_cov_3.245303"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PL106_09635"
     CDS             complement(<1..844)
                     /locus_tag="PL106_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33369.1"
                     /translation="MLTRAAQVPNTGKPEDMEEAVGDAVRALDNLASEAGDQSERAQE
                     LADSLVMELCVREANTFWLAGMAANAVTSYRQALDRGDRSHTLKGRVHDLVGNLATAK
                     REAEGPEAAIKVYDEYIKKAVGAKDSVALRDLLNRQSILCQENRDFEQAIAIMERVQA
                     IDDRGSDVQNKIRTSLKLSRLHEMAGSPDAYTYARTAYELAKKNDDEYLLLQACDLLI
                     GSFLATDTAQCRILLQEALNTAVERDKATDRATFHDRYGRFEMRLRHWDEAFKQLQQG
                     LDVLG"
     gene            1090..>1971
                     /locus_tag="PL106_09640"
     CDS             1090..>1971
                     /locus_tag="PL106_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33370.1"
                     /translation="MGITYSRGSIATLIIGLAFTGSVQAQHTIIKKSGGQSAGKVVGI
                     AAGLLRYQLDPTSDEVSTWPCNDVLAIIDERLVVHAGNPCEAVGELGKAQRNCASLLM
                     KDGSVVKCQVTSELDTYRKIRTKDGERTEPDDDVVGIINGDEVDFTDMKFALRFMSDA
                     NTVRMINDVSQCPSGYGEVGTTRSAKKNLPRVEAAQKKAAIARAKANSGTVPVVKAPV
                     DTSRGLMEEFDAEKLTAIGVDKVRRLEAYMKVITDGKVAQAARAEQCKQADALFGDAY
                     ENKVQSSTVPADGGPART"
BASE COUNT          390 a          572 c          608 g          401 t
ORIGIN      
        1 atccgagcac gtccagcccc tgctgaagtt gtttgaatgc ttcgtcccaa tgccgcaaac
       61 gcatctcgaa gcggccgtac cggtcgtgga aggtcgcacg atccgtggct ttgtcccgct
      121 ctacggccgt gttgagggct tcctgcagga gaatgcggca ttgtgccgta tcggtggcca
      181 ggaaggaccc gatcagcagg tcgcacgctt gaagcagcag atattcatcg tcattcttct
      241 tggccagttc gtacgcggta cgggcatagg tgtacgcatc cgggctaccg gccatttcat
      301 gaaggcggct cagcttcagt gatgtgcgga tcttgttctg gacatcgctg ccacggtcat
      361 cgatcgcctg tacacgctcc atgatcgcaa tggcctgctc gaaatccctg ttctcctggc
      421 acagtatgct ttgccggttg agcaggtcgc gcaacgcgac gctgtccttg gccccgacgg
      481 cttttttaat gtactcgtca tagaccttga tcgcggcttc cgggccttcc gcctcccttt
      541 tggccgtggc caggttgccc accagatcat gcacccttcc cttgagggtg tgagagcgat
      601 caccacggtc caaggcctgg cggtagctcg ttacggcgtt ggcggccatc ccggccagcc
      661 agaacgtgtt ggcttcccgc acacacaatt ccatgaccag gctgtccgcc agttcctgtg
      721 ctcgttcgct ctgatccccg gcttcggagg ccaggttgtc cagtgcccgg accgcatcac
      781 caacggcctc ctccatgtcc tcgggctttc cggtgttggg cacttgtgcg gcccgtgtca
      841 gcatggcttc ccgccagttg gtagcaggtg tctgggatgt ggccggcccc atcagcgcgc
      901 aacaaacggc gagcaagctc gaccgcagga tcggcccggc atttgccagc ggacgacgcc
      961 accctttgcg catgggcggt gaaggtaagc aggtccggtc tacctctcca gtggtcgggt
     1021 tccggtcctt ttcacctaaa ttttcgcgcc ttttcagaac gggcaccaaa gcacaaagcg
     1081 gacacgaaca tggggatcac gtactcgcgc ggcagcatcg ctacgctcat cattggactg
     1141 gctttcaccg gtagcgtaca agctcagcat acgatcatca agaagtcagg agggcaatcg
     1201 gcaggcaagg tggtcggcat cgctgcgggg ctgttgaggt accagctcga tccgacctcg
     1261 gacgaagtga gcacctggcc gtgcaatgat gtcctggcca ttattgatga acgtctggtc
     1321 gttcatgcgg gcaacccctg cgaggcggtg ggcgaattgg gcaaagctca acgcaattgt
     1381 gcatcactcc tgatgaagga cggctcggtg gtgaaatgcc aggtgacgtc cgaactcgac
     1441 acctatcgga agatccgtac gaaggatggc gagcgcacgg aacccgacga cgacgtggtg
     1501 ggcatcatca atggggatga agttgatttc accgatatga agttcgccct gcggttcatg
     1561 tccgatgcga ataccgtgcg catgatcaat gatgtatcgc aatgtccgag cgggtatggt
     1621 gaggtcggga ccactcgttc agccaagaag aacctgccgc gggtggaagc ggcgcagaag
     1681 aaagcggcga tagcgcgagc gaaagccaat agtggtacgg tgccggtggt caaggctcca
     1741 gtggatacct ccagggggct catggaggag ttcgatgctg aaaaactcac tgcgatcggt
     1801 gtggacaagg tccggcggct ggaggcgtac atgaaagtca tcaccgatgg gaaggtcgca
     1861 caggcggccc gcgccgaaca gtgcaaacag gccgacgccc tcttcgggga cgcctatgag
     1921 aacaaggtgc aaagcagtac cgtgcccgcc gatggggggc ctgccagaac a
//
LOCUS       DAOQOC010000588         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39086_length_1971_cov_2.696764, whole genome shotgun sequence.
ACCESSION   DAOQOC010000588 DAOQOC010000000
VERSION     DAOQOC010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39086_length_1971_cov_2.696764"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PL106_09645"
     CDS             complement(<1..393)
                     /locus_tag="PL106_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33371.1"
                     /translation="MAKQKDKKETKRKSILDKGAHEFMSRYLNNASPTGFESSGQRIW
                     LDYLKPYIDTHFTDPYGTAVGVINPDAKYKVVIEAHADEISWFVSYITKEGFIYVRRN
                     GGSDHTIAPSKRVNIHTAAFREIGVGVKL"
     gene            587..>1971
                     /locus_tag="PL106_09650"
     CDS             587..>1971
                     /locus_tag="PL106_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33372.1"
                     /translation="MNARAYLTLLLSGTFGLLAAQNGPRTNTPFDKAHFADAEGLKAA
                     LAAIRKGDGLFRHGGRDTDAALLAFEQAYAFNRDNADLNLKIGLCHLNGRQHHLSLPF
                     FQAALALDNTIPRIHFLLGAAYQLNARWDEAIAEYREHVTASGGFPDVEPLYNTAERR
                     MIQCKNGRSLQSASLTGQVTNLGDAINSTVADYGVLTSLEGDRLSYTSRRSLTADTRI
                     NKNTGDPFEDIFVVERTEMGWGTPRPIGTPVNSELNDASVGLFPDGRTLLIYRDAKGP
                     GDLYTSTLSGNTWSTPVPLPDAINSTANETSAWPGPDGKTMYFVSDREGGVGGQDIWI
                     SQWDANAGTWGPPRNPGPSINSIEDEDGVFMSADGRTIYFSSKGHICMGGFDVFKTTL
                     VNGQWSKPENLGWPVNSPDDDLFFVLNANGTTGYLSSVRPNGLGEDDLYRVDILPDAK
                     AEETASVGGIGS"
BASE COUNT          365 a          627 c          611 g          368 t
ORIGIN      
        1 gagcttcacg ccaacgccga tctcgcggaa ggcggcggtg tggatgttca cgcgcttgct
       61 cggtgcgatg gtgtggtcgc tgcctccgtt gcggcgcacg tagatgaagc cttccttggt
      121 gatatagctg acgaaccagc tgatctcgtc ggcgtgggcc tcgatgacca ccttgtactt
      181 cgcatcgggg ttgatcacgc cgaccgcggt cccgtagggg tcggtgaagt gcgtatcgat
      241 gtacggtttc aggtagtcca gccagatgcg ctgaccgctg ctctcgaagc cggtcgggct
      301 ggcgttgttc aagtagcgcg acatgaactc gtgcgcgccc ttgtcgagga tgctctttcg
      361 cttggtctcc ttcttgtctt tctgcttggc catgtgggtt ggtagctgtg ggcggcgaag
      421 ctacctgtgc agggcgctgg gccgcgcacc ggtccacgat gcgccgtgcc ctgtcgtgga
      481 tgccggaggc agccctcggc ccgcctccat tgcggagaac cggtccgagc gaacctcttg
      541 ccgcgctcac cgtaaaaagg ccgctctatc cgtaccgacc ctcaacatga acgcacgcgc
      601 atacctcacc ctgttgttgt ccggaacctt tggcctgttg gccgcacaga acggaccccg
      661 gaccaacacg cccttcgaca aggcgcactt cgccgatgcc gagggactga aggcggccct
      721 ggcggcgata cggaaggggg atgggctctt ccgccatggt gggcgcgaca cggatgcggc
      781 cctgctcgca tttgagcagg catacgcgtt caaccgggac aatgccgacc tgaacctgaa
      841 gatcggattg tgccacctca acggtcggca gcaccatttg tccctgccct ttttccaggc
      901 cgcgttggcg ctggacaaca cgatcccgcg catccacttc ctgttgggcg ccgcgtacca
      961 gctgaatgcc cgctgggacg aggcgatcgc cgaatatcgt gagcacgtga ccgcatcagg
     1021 aggcttcccc gatgtggaac ccttgtacaa taccgccgag cgccgcatga tccaatgcaa
     1081 gaacgggcgg tcccttcaaa gtgcttcgct caccgggcag gtgaccaacc tgggggacgc
     1141 gatcaacagc acggtggcgg actacggtgt gctcacctca ttagagggtg atcggctcag
     1201 ctacaccagt cgccggagcc ttaccgccga cacccggatc aataaaaata ccggtgatcc
     1261 cttcgaggac atcttcgttg tggaacggac ggaaatgggt tggggcaccc cccgacccat
     1321 tggaaccccc gtgaattcgg agctgaatga tgcctcagtg ggtctcttcc ccgatgggcg
     1381 caccctgttg atctaccgcg acgccaaggg cccgggcgac ctctacacga gcacgttgag
     1441 cggcaacacc tggagtacgc ccgtgccact tcctgacgcg atcaactcca ccgccaacga
     1501 gaccagcgca tggccgggcc ccgacggtaa aacgatgtac ttcgttagcg accgcgaggg
     1561 aggtgtgggc ggacaggaca tctggatcag tcagtgggac gccaacgcgg gcacctgggg
     1621 cccaccccgc aaccccggcc cgtccatcaa ttcgatcgag gacgaggatg gtgtgttcat
     1681 gtccgccgac ggccgcacca tctacttcag cagcaagggc cacatctgca tgggcggatt
     1741 cgatgtgttc aagaccaccc tcgtgaacgg gcagtggagc aagccggaga accttgggtg
     1801 gcctgtgaac agtcccgatg atgacctctt cttcgtgctc aacgccaacg gcacgaccgg
     1861 atacctcagc agcgtgcgcc ccaatggcct gggcgaggac gacctctacc gcgtggatat
     1921 cctgccggac gccaaagcgg aggagaccgc ctcggtgggc ggtatcggct c
//
LOCUS       DAOQOC010000589         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39111_length_1970_cov_3.910183, whole genome shotgun sequence.
ACCESSION   DAOQOC010000589 DAOQOC010000000
VERSION     DAOQOC010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39111_length_1970_cov_3.910183"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PL106_09655"
     CDS             <1..1117
                     /locus_tag="PL106_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNA33373.1"
                     /translation="EGGGQVGDQGQLVQGADQVEVIDTKRENGVIVHFTKMLPKNVEA
                     TLTVRVDAQRRGLTARNHTATHLLHHALRKHLGTHVEQKGSLVAPDRLRFDISHFAKV
                     TPEELTAIEQEVNAMIADDLVFEDLRNVPIEQARSMGAMALFGEKYGDAVRVVKFGPS
                     IELCGGTHVPRTGTIGPFRIVSESALAAGIRRIEAIPSVEAERMINAKLARLDAISAL
                     LKNPADPVAAVEKLLEQNAALGKEVEKAAKERVRAYTASLPARARSNAKGVKVLVERL
                     DLDAQSLKDLGYALREQHADLALVAGSVVDGKPLLAVSLGKDFSSATGLKAVDIIKRI
                     SPSIKGGGGGQPDFATAGGKEPEGMAAALKLADELLS"
     gene            complement(1191..1628)
                     /locus_tag="PL106_09660"
     CDS             complement(1191..1628)
                     /locus_tag="PL106_09660"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNA33374.1"
                     /translation="MELITHFFEYLQNAGGTGLWYTLGFIILVGVVAQWRLFDKAGLP
                     GWTCVVPILGQVNYMKMVGRPAWHLLLFLIPGYNVYLLFKVWIETAQSFGKRTMLDYV
                     LVIVLNGLYIFNLGMSYETRYLGPVYGKHIGTHHGSSANPSLA"
     gene            1767..>1970
                     /locus_tag="PL106_09665"
     CDS             1767..>1970
                     /locus_tag="PL106_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33375.1"
                     /translation="MERGTAPCQAGQPPRAGLYRASDAWIKKPLAGSAERTTFAAHFE
                     DPMYAIVDIAGQQFKVKAGQKLKV"
BASE COUNT          430 a          614 c          601 g          325 t
ORIGIN      
        1 ggaaggcggc ggccaggtgg gcgaccaggg ccagctcgtc caaggcgccg atcaggtgga
       61 ggtgatcgac accaaacgcg agaacggcgt catcgtccac ttcaccaaaa tgcttccgaa
      121 gaacgtggag gccaccctga ccgtgcgggt ggacgcccag cgtcgcggcc tcaccgcccg
      181 caaccacacg gccacccacc tgctgcacca cgcgctgcgc aagcacctcg gtacgcacgt
      241 ggagcagaaa ggctcgctgg tggcgcccga tcgcctgcgt ttcgacatca gccacttcgc
      301 caaggtgacg cccgaggaac tgaccgcgat cgagcaggag gtgaatgcga tgatcgccga
      361 cgacctcgtc tttgaggacc tgcgcaacgt gcccatcgag caggccagaa gcatgggggc
      421 catggcgctc ttcggcgaga agtacggaga tgccgtgcgc gtggtgaagt tcgggcccag
      481 catcgaattg tgcggcggta cgcatgtgcc gcgtaccggt accatcggcc ccttccgcat
      541 cgtgagcgag agcgcactgg ccgcgggtat ccgacgtatc gaggccatcc ccagcgtgga
      601 ggccgaacgc atgatcaacg cgaaactggc cagactggac gccatcagcg ccctgctgaa
      661 gaaccccgcc gatcccgtgg ccgccgtgga aaaactactg gaacagaacg ccgcgctggg
      721 caaggaggtg gaaaaggccg caaaagagcg tgtgcgtgcc tacacggcat cgctcccggc
      781 cagggccagg tcgaacgcca agggcgtgaa agtgctggtg gaacgcctcg acctcgatgc
      841 gcagagcctc aaggacctgg gttacgcctt gcgcgaacaa cacgccgacc tcgccctggt
      901 ggccggttcg gtggttgacg gaaaaccgct gcttgcggtg tccctgggca aggatttcag
      961 ttcggccacg ggactcaaag ccgtggacat catcaaacgg atcagtccat cgatcaaggg
     1021 cggcggcggt ggccagcccg acttcgccac ggctggtggt aaagagcccg aaggcatggc
     1081 tgccgcgttg aagttggccg acgaattgtt gtcctgatcc cttgaacgga aaccggcccg
     1141 gctccttccc aacggaacga tccaggccgg tcagatcgga aagaaggccc tcaggccaaa
     1201 ctgggattgg cggaactgcc gtgatgcgtc ccgatatgct tgccgtatac cgggcccagg
     1261 tatctcgttt cgtagctcat gcccagattg aagatgtaca gtccattgag cacgatcacc
     1321 agcacgtaat ccagcatggt gcgcttgccg aagctctggg ccgtttcgat ccacaccttg
     1381 aacaagaggt acacgttgta ccccggaatg aggaacaaca ggaggtgcca tgccgggcgc
     1441 ccgaccattt tcatgtagtt cacctgcccc aggatgggca ccacgcaggt ccagccgggc
     1501 aggcccgcct tgtcgaacag ccgccattgg gccacaacac ccacgaggat gatgaagccc
     1561 aaggtgtacc acagccctgt gccccccgcg ttctggaggt attcaaagaa atgggtgatc
     1621 agttccatga ccgatgagtt tggttttcgg actcgttacg ggatccttgc cgatccaata
     1681 gggtagacgg cgaaaaatga ccccggttgc cttaaaataa cggccccggc cccaccttct
     1741 tatgcacctt ttgctcaaca ccctccttgg aacgaggaac ggcaccttgc caagccggac
     1801 agcccccccg ggcaggcctg taccgggcat cggacgcttg gataaaaaag ccgcttgccg
     1861 gatcggcgga acgaactaca ttcgcagccc atttcgaaga ccctatgtac gccattgtgg
     1921 acatcgccgg ccagcagttc aaagtaaagg ccggccaaaa gctgaaagtg
//
LOCUS       DAOQOC010000590         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39123_length_1970_cov_3.063708, whole genome shotgun sequence.
ACCESSION   DAOQOC010000590 DAOQOC010000000
VERSION     DAOQOC010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39123_length_1970_cov_3.063708"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PL106_09670"
     CDS             complement(<1..816)
                     /locus_tag="PL106_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNA33376.1"
                     /translation="MMLLEVNVMLSATSLVTWLVALTLVLLVGAIYMASKARMLLREL
                     HGVEQPSPTNFFLVLMLMLAAAGAVVYLLKMGIEAQSGMLNTMMFIALPYVALAIFFI
                     GSIYRYRNRGFQVSSLSSEFLERKKLFWGSQPFHWGLLVLFFGHLIAFLFPRTVMAWN
                     GQPVRLLILEYSSFAFGLATLLGLMLLVRRRLGNKRVLIVSNRMDMLVYTVLFTQIIS
                     GLGVAFFARWGSSWFASSVTPYLRSLFAFNPDITAVSAMPWVIQVHIISAFSII"
     gene            complement(813..1493)
                     /locus_tag="PL106_09675"
     CDS             complement(813..1493)
                     /locus_tag="PL106_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33377.1"
                     /translation="MAAELFRYPGVDLNDRVTALYDVLGTRYPEAAGTLSPFVEWVAT
                     TPAHRVEEVFSRTFHVQAICYLDIGFVIFGEDYKRGEFLVNMKQEQSLAGNDCGEELP
                     DNLVNMLTLLPLLKDDAFRAELAGRVMMPALRRMLAEFSPEKSRLRTNMLRRKHNALI
                     MPEEQHMNVYHHALLALFQMFAQDFEEVALETYRPNGHDPLRAAVPASECGTCTVPHS
                     PLIKTMKP"
     gene            complement(1526..>1970)
                     /locus_tag="PL106_09680"
     CDS             complement(1526..>1970)
                     /locus_tag="PL106_09680"
                     /inference="COORDINATES: protein motif:HMM:NF026061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33378.1"
                     /translation="NLFYVPPMLPAMASVDADGVYDSHTESLWAGIEKSRLPMQYLAS
                     LFTAGDTERMKLVLKKLLAVKISRRNTTVGDLSAKEVADALEGGRTNAKEADAIFRLT
                     SLATFEERFVIPPAHREESIEMLEDTGDVKGNTGFGFKLKPARGL"
BASE COUNT          404 a          592 c          614 g          360 t
ORIGIN      
        1 gatgatggag aacgcggaga taatgtggac ctggatgacc cacggcatgg cgctcactgc
       61 tgtgatgtcc gggttgaagg cgaagagcga acgcaggtag ggcgttacgc tggaggcgaa
      121 ccacgacgag ccccaacgtg cgaaaaaggc cacccccaga ccactgatga tctgcgtgaa
      181 aagcacggtg tacaccagca tgtccatgcg gttgctcacg atcagcacgc gtttgttgcc
      241 cagccgccgc cgcaccagca gcatcaatcc gagcaatgtg gccagtccga aggcgaacga
      301 actgtactcc aggatgagca accgcacggg ttgcccgttc caagccatca ccgtgcgtgg
      361 gaaaaggaag gcgatcagat ggccgaagaa gagcaccagc aggccccagt ggaagggctg
      421 gctgccccag aagagtttct tgcgctccag gaattccgag gagagcgaac tcacctggaa
      481 accccgattc cggtagcgat aaatggatcc gatgaagaag atggccaggg ccacgtacgg
      541 cagggcgatg aacatcatgg tgttcagcat gcccgactgg gcctcgatcc ccatcttcaa
      601 cagatagacc accgctcccg ctgcggccag catcagcatg agcaccagga agaagttggt
      661 gggcgatggt tgctccacac cgtgcagctc gcgcaggagc atcctggcct tggaagccat
      721 gtaaatggcg cccacgagca gcaccagcgt gagggccacg agccacgtga ccaaggatgt
      781 ggcggaaagc atcacgttta cctcaagcag catcatggtt tcatggtttt gatcaggggt
      841 gagtggggaa cagtgcaggt gccgcattcc gatgcgggaa ccgctgcccg caacggatca
      901 tggccgttgg gccggtacgt ttccaatgcg acctcctcga agtcctgcgc gaacatctgg
      961 aacagggcca gcaaggcgtg gtgatatacg ttcatgtgct gctcctccgg catgatcagg
     1021 gcattgtgct tgcgtcgcag catgttggtg cgcaacctgc tcttctccgg gctgaactcg
     1081 gccagcatcc ggcgcagcgc gggcatcatc acacgaccgg ccagttccgc ccggaacgca
     1141 tcatccttga gcagcggcag cagcgtgagc atgttcacca ggttgtccgg cagttcctct
     1201 ccgcaatcgt tgccggccag ggactgctcc tgcttcatgt tcaccaggaa ctcgccgcgc
     1261 ttgtagtcct cgccgaagat gacgaaaccg atatccaggt agcagatggc ctgcacatgg
     1321 aacgttcggc tgaagacctc ctccacgcgg tgcgccggcg tggtggccac ccattcaaca
     1381 aagggcgaga gggttccagc cgcctccggg taccgcgtac ccagtacgtc gtacaatgcg
     1441 gtcacacggt cgttgaggtc cacaccggga tagcggaaga gctccgctgc caaggcgtat
     1501 tgtccgtatg gccgtgcgct gcccatcaca gtccgcgtgc cggtttcagt ttgaagccga
     1561 agccggtgtt gcccttcacg tcgcccgtgt cctccagcat ctcgatgctt tcctcgcggt
     1621 gcgcgggcgg gatcacgaag cgctcctcga aggtggccag cgaggtgagg cggaaaatcg
     1681 catcggcctc cttggcgttc gtgcggccac cttccaacgc atcggccact tccttcgcgc
     1741 tgaggtcgcc tacggtggtg ttcctgcgac tgatcttcac ggccagcagc ttcttgagca
     1801 ccagtttcat gcgctcggtg tcgcctgcgg tgaagagcga agcgaggtac tgcatgggca
     1861 ggcggctctt ctcgatgccg gcccagagcg attcggtgtg ggagtcgtac acgccatcgg
     1921 cgtccacact ggccatggcg ggcaacatgg gcggtacgta gaacaggttg
//
LOCUS       DAOQOC010000591         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39217_length_1968_cov_2.623105, whole genome shotgun sequence.
ACCESSION   DAOQOC010000591 DAOQOC010000000
VERSION     DAOQOC010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39217_length_1968_cov_2.623105"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PL106_09685"
     CDS             <1..818
                     /locus_tag="PL106_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA33379.1"
                     /translation="VMRMLVGEESYEGEIKLGHQVTIGYYAQDMPDRLDPTLTIMETA
                     EQAASDDNRPKVRGMLGAFLFSGDDVDKKVKVLSGGERARVALCCMLLRPLNFILLDE
                     PTHHLDIQSKDVLKQALKDYDGTFLIVSHDRDFLHGLTNRLLEVKDGRLRDHHMDILE
                     LIDRNKASASMNDTVKQVAVKQRTAGSKVDNRLPQKEQEKELRRLRNAVQRCEQEMAR
                     LESEQKQLQDKLADNPSEKDYARLAELAGLIEQRMKDWERAGNELEALAQEIG"
     gene            901..973
                     /locus_tag="PL106_09690"
     tRNA            901..973
                     /locus_tag="PL106_09690"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:934..936,aa:Met,seq:cat)
     gene            1027..1902
                     /gene="era"
                     /locus_tag="PL106_09695"
     CDS             1027..1902
                     /gene="era"
                     /locus_tag="PL106_09695"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNA33380.1"
                     /translation="MSHRAGYVNIIGHPNVGKSTLLNALLGEKLVIVNPKAQTTRHRI
                     LGILNGDDHQLILSDTPGILDPQYPLHKSMMKAVDEALQDADILLVLVELGENPERAE
                     AVVKRAERFGGPLFVLVNKMDLGSEAELAAALETWQGIFPKAKVVPISALHGFHVDAL
                     RERLIELLPEHPPYFPKDDLSDRDLRFFTSEMIREKVLALYKQEIPYSTQVVVNSYEE
                     GEELVKIQADVVVMRESQKGIVIGRGGGALRQLGTEARKAIEKFCGTKVFLDLKVKVD
                     PDWREDDKKLKRYGY"
BASE COUNT          444 a          575 c          623 g          326 t
ORIGIN      
        1 ccgtgatgcg catgctcgtg ggtgaggaga gctacgaggg tgagatcaag ctgggccacc
       61 aggtcactat cggctactac gcccaggaca tgcccgatcg cttggacccc accctcacga
      121 tcatggagac cgccgagcag gccgcaagcg acgataaccg ccccaaggtg cgcggcatgt
      181 tgggggcctt cctcttcagc ggcgatgatg tggacaagaa ggtgaaggtc ctcagcggtg
      241 gcgaacgggc gcgtgtggcc ctgtgctgca tgctgttgcg tccgctcaac ttcatcctgc
      301 tcgacgagcc tacgcaccac ctggacatcc agagcaagga tgtgctcaaa caagccctca
      361 aggactacga tggcaccttc ctcatcgtga gccacgaccg cgacttcctc cacggactca
      421 ccaaccgcct gctcgaggtg aaggatgggc gcctgcgcga tcaccacatg gatatcctgg
      481 agctgatcga ccgcaacaag gcgtcggcga gcatgaacga tacggtgaag caggtggcgg
      541 tgaaacagcg gaccgccgga tccaaggtgg acaaccggct gccacagaaa gaacaggaaa
      601 aggaactccg ccggctcagg aatgcggtgc agcgttgcga gcaggagatg gcccggctgg
      661 agagcgagca gaagcagttg caggacaagt tggccgacaa cccgtccgag aaggactacg
      721 ctcgactggc cgaactcgcc ggactcatcg agcagcgcat gaaggactgg gagcgcgctg
      781 gcaacgagtt ggaggccctt gcacaggaga tcggctgacc ggaccgcaac aatcagggtt
      841 ggcctctctt cacccgatat tctgaattcg cccggttgaa ctatattcgc acccccaaac
      901 tgcggggtgg agcagatggt agctcgtcgg gctcataacc cgaaggccgc aggttcgagt
      961 cctgcccccg ctacaaggaa gagccccgac gcgagtcggg gttcttcgct ttcaacgcga
     1021 gcgcccatgt cccatcgcgc cggatacgtc aacatcatcg gccaccccaa cgtgggcaag
     1081 agcaccctgc tcaatgcgct gttgggggag aagctcgtga tcgtgaaccc caaggcccag
     1141 accacgcggc accggatcct gggtatactc aatggtgatg atcaccaact gatcctttct
     1201 gatacgcccg gcatcctcga tccgcagtat ccgctccaca agagcatgat gaaggccgtg
     1261 gatgaggcac tacaggatgc ggacatcctc cttgtgctgg tggagctggg ggaaaaccct
     1321 gaaagggccg aggccgtggt caaacgggcg gagcgcttcg gcggtcccct tttcgtcctg
     1381 gtgaacaaga tggatctggg cagcgaagct gaactcgccg cggccctgga aacctggcaa
     1441 gggatcttcc cgaaggccaa ggtggtgccc atctccgcac tgcacggctt ccatgtggat
     1501 gccctgcgcg aacgtctgat cgaactgttg ccggagcatc cgccttactt tccgaaggat
     1561 gacttgagtg accgcgacct gcgcttcttc accagcgaga tgatccgcga aaaagtactc
     1621 gccttgtaca agcaggagat cccgtacagc acccaggtgg tggtgaacag ctacgaggag
     1681 ggcgaggaac tcgtgaagat ccaggccgat gtggtggtga tgcgcgagag ccagaaaggc
     1741 atcgtgatcg gcaggggcgg gggggccctg cgccaactcg gtaccgaagc gcgcaaggcc
     1801 atcgaaaagt tctgtggcac caaggtcttc ctcgacctga aggtgaaggt ggatcccgac
     1861 tggcgggagg atgacaagaa attgaaacga tacggatact gaatgagagt ggcgagagac
     1921 gagagacgag aggcgagtgc gcactgtcct ggacagcgca ctcgcctc
//
LOCUS       DAOQOC010000592         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39220_length_1968_cov_2.484056, whole genome shotgun sequence.
ACCESSION   DAOQOC010000592 DAOQOC010000000
VERSION     DAOQOC010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39220_length_1968_cov_2.484056"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1745)
                     /locus_tag="PL106_09700"
     CDS             complement(159..1745)
                     /locus_tag="PL106_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33381.1"
                     /translation="MRPNQTAGRDRLIQQTLRDQARLYFAAAGNTTTDYEQAHITTAA
                     PIDLTGHPNVILSFETQYRRYNDEQTFLVVSTDGNFPTLDDPLMDISAMPGVFRVWED
                     GELTQGVSPGNPTVREFNISAAAGDAPQVWVRLQFTGIYGYTWYIDDISIYDQYQYDS
                     YMLNGFVSHRGDGSEFGRIPSTQLGSQMNVGAWVINKGYDVLTNVTASASSTAFATVS
                     ANAATLNPGDTLFFNEWASVAGLATGAYTVDYTVTSDQSGSEGNMANNNWQRTFEVTD
                     DMYSLDQIGGHPEGTEVLTYFGTNQFTGGADGVMGFTYYPIIEEFAVTGMQIELANGT
                     VVGGTLQVSLHDSTTLMADDPTPIEVSNDHEVTQADLDAGVINVAFDNPVTLDPGAYY
                     AGITMYSNENANDIRVLDDLTVPQPGISSALFLDATLYSNINALALRLSSDPTIGMEE
                     SRLTGVGVYPNPSTGLVNVAFVNTGKYTVDVLNSLGQTVQRTRMNGTSTMDLSTMPKG
                     VYSLRISNGTATTVDRVVLQ"
     gene            1754..>1968
                     /locus_tag="PL106_09705"
     CDS             1754..>1968
                     /locus_tag="PL106_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33382.1"
                     /translation="MGLYRPPDAPPLTPLEAAVLWDADKLTKLGVQALAYSLSMNFLR
                     GLTLPQRRSNMLEFTQSVLQRTVQSMNT"
BASE COUNT          332 a          558 c          661 g          417 t
ORIGIN      
        1 gctggcgaaa gtaggtgctc ggcctgtttt ttcgtcaccg gctgcacgga ccgtcccggt
       61 gcaggcacga acggtcgtcc gaacgcagga ccggtacggg tatgaacgac cggcgccccc
      121 cgatgatcgg gaggcgccgg tccagtcgtc agcaggtttt actgcagcac aacacggtcc
      181 accgtggtgg cggtaccgtt ggagatgcgc aggctgtaaa cacctttggg catcgtgctc
      241 aggtccatgg tgcttgtgcc gttcatgcgc gtgcgctgca cggtctggcc caggctgttc
      301 aggacatcca cggtgtactt gccggtgttc acgaaggcca cgttcaccag gccggtgctc
      361 ggattggggt atacccctac gcccgtgagg cggctctcct ccatgccgat ggtggggtcg
      421 gagctcaggc gaagtgccag ggcgttgatg ttgctgtaca gggtggcgtc caggaacaat
      481 gcactgctga tcccgggttg tggcacggtc aggtcgtcca gtacgcggat gtcgttggcg
      541 ttctcgttgc tgtacatggt aatgcccgcg tagtaggcac cgggatcaag ggtcaccggg
      601 ttgtcaaaag ccacgttgat cacaccagcg tcgagatcgg cttgggtcac ctcgtgatca
      661 ttggacacct cgatcggggt ggggtcgtcg gccatcaggg tggtggagtc gtgcaggctc
      721 acttgaagcg taccgccgac aaccgtgccg ttggcgagct cgatctgcat gcccgtaacg
      781 gcgaattcct cgatgatcgg gtagtaggtg aagcccatca caccatcggc tccgccggtg
      841 aattggttgg taccgaagta cgtcagaact tcagtgccct ccggatggcc accgatctgg
      901 tcgagcgagt acatgtcgtc ggtcacctcg aaggtgcgct gccagttatt gttggccatg
      961 ttgccttcgc taccgctctg gtcactggtc acggtatagt ccacggtata ggcaccggtc
     1021 gccaggccgg ccacggaagc ccattcgttg aagaagaggg tgtcgccggg gttgagtgtg
     1081 gcggcgttgg cgctcacggt ggcgaacgcg gtcgagctgg cgctggcggt cacgttcgtc
     1141 agcacgtcgt agcccttgtt gatcacccaa gcgcccacgt tcatctggct gccgagttgc
     1201 gtgctcggga tacgtccgaa ctcgctcccg tctcccctgt gcgaaacgaa accgttgagc
     1261 atgtaggaat cgtactggta ctggtcgtag atgctgatgt cgtcgatgta ccaggtgtag
     1321 ccgtaaatac cggtgaattg caggcgcacc cacacttgtg gcgcgtcgcc cgctgcagcg
     1381 ctgatgttga attcgcgtac ggtggggttg ccgggcgaaa cgccttgggt gagttcgcca
     1441 tcctcccaaa cgcggaacac accgggcatg gcgctgatgt ccatcagtgg atcgtccagc
     1501 gtggggaagt tgccatcggt gctgaccacc aggaacgtct gttcgtcgtt gtaccgacgg
     1561 tactgcgttt cgaacgagag gatcacgttc gggtggccgg tcaggtcgat cggagcggcc
     1621 gtggtgatgt gggcttgctc gtagtcggtg gtggtgttgc ccgccgccgc aaaatagagg
     1681 cgggcttggt cgcgcagggt ctgctgaatg aggcggtcgc gcccagcggt ctgattgggc
     1741 cgcacagggt cacgtgggcc tttatcgccc tcccgatgcg ccgccgctga cgccgctcga
     1801 agctgccgtg ctgtgggatg ccgacaagct gaccaagctg ggcgtgcagg cactagccta
     1861 cagcctgagc atgaactttc tgcgtgggct gacgctgccg cagcggcgca gcaatatgct
     1921 ggagtttacc caatccgtgc ttcagcgcac ggtgcagagc atgaatac
//
LOCUS       DAOQOC010000593         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39278_length_1966_cov_3.408163, whole genome shotgun sequence.
ACCESSION   DAOQOC010000593 DAOQOC010000000
VERSION     DAOQOC010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39278_length_1966_cov_3.408163"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PL106_09710"
     CDS             complement(<1..840)
                     /locus_tag="PL106_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA33383.1"
                     /translation="MLNKLILFLASANVAVVGYGQARLVAQVNDGADNALPFAQVSVN
                     DGVFGTQADEHGQFTLAGLRPGDAPLRIVLVGYRTLDTVITLVAGDNRMTFALDPDDH
                     LLRDVEVSAIRAGDRAPFARSTVTKAEIRRSNTGVDLPYLLDGQPSTVVTSDGGTGIG
                     YTSMRIRGTDATRTNITLNGVPFNDAESQGAFLVNLPDMATSTEDIEVQRGVGTSTNG
                     PAAFGASINLRTTTVQRQPWGLVGVSGGSYGTQRYSVSAGTGLIGDHFSLDMRLSSIT
                     TDGY"
     regulatory      complement(876..975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1013..>1966)
                     /locus_tag="PL106_09715"
     CDS             complement(1013..>1966)
                     /locus_tag="PL106_09715"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNA33384.1"
                     /translation="AMHIAAYKQTVEVTLPGVKKLRDTLNKKAKAFDKIVKTGRTHFM
                     DATPLTLGQEFSGYVQQLDNGLRAVNNALEMIRELALGGTAVGTGLNAPKGYSVLVAK
                     KIAELTGLPFITAPNKFESLAAHDAMVELSGAFKRLAVSLMKIANDIRILSSGPRSGI
                     GEIVIPDNEPGSSIMPGKVNPTQPEALTMVCAQVIGNDVAVSVGGMQGHFELNVVKPL
                     IAANVLQSGRLLGDACVSFTDKCAVGIEPNNEVIKRHLENSLMLVTSLNTHIGYYKAA
                     EIAKKAHKEGTTLKQSALALGYVSAEDFDKWVDPAKMCSEG"
BASE COUNT          318 a          617 c          631 g          400 t
ORIGIN      
        1 gtacccatcg gtggtgatgg aggagaggcg catatcgagg ctgaaatgat ccccgatgag
       61 gccggtaccc gcgctcacgc tgtagcgttg cgtaccgtac gatcccccgc tcaccccgac
      121 caggccccag ggttggcgtt gcaccgtggt ggtgcgcagg ttgatgctgg ccccgaaggc
      181 ggccggtccg ttggtgctgg tgcccacgcc gcgctgcacc tcgatgtcct ccgtgctggt
      241 ggccatgtcg ggcagattca ccagaaaagc tccctggctc tcggcatcgt tgaacggcac
      301 gccattgagc gtgatgttgg tgcgggtggc atcggtaccc cgtatgcgca tggaggtgta
      361 gccgatgccg gtgccgccgt cgctggtgac cacggtactg ggttgcccgt cgagcaggta
      421 aggcaggtcc acgccggtgt tgctgcggcg gatctcggcc ttggtcaccg tgctgcgggc
      481 gaagggcgcg cgatcacccg cacggatggc actgacctcc acatcgcgaa gcaggtggtc
      541 gtcggggtcc agtgcgaagg tcatccgatt gtcgccggcc accagggtga tcaccgtatc
      601 cagggtacga tagcccacca gaacgatgcg caggggcgca tcgcccgggc gcaggcccgc
      661 aagggtgaac tgtccgtgtt catccgcctg ggtgccgaat acgccatcgt tcacactcac
      721 ctgtgcgaac ggcagcgcat tgtccgcccc atcattcacc tgtgccacca gccgtgcctg
      781 cccgtacccg acaacggcaa cgttggctga cgccagaaag aggatcaatt tgttcagcat
      841 gccctgtgat cagtgtcctc acggggcgtc cccgaaaagt cgggcgactc attcccttgg
      901 cggcatgacc cgcccaggtt cttcgggtat gatctcagcc ctcaccttgc gaagaggaca
      961 ccccgttgga cggggcgaag gtaggcggat ccgggtgtgt ccgatcgggg gatcagccct
     1021 cgctgcacat cttggccgga tccacccact tatcgaagtc ttcggccgac acgtaaccga
     1081 gtgcgagcgc ggactgcttg agcgtggtgc cttccttgtg cgccttcttg gcgatctcgg
     1141 ccgccttgta gtatccgatg tgggtattga gactggtcac aagcatcagc gagttctcca
     1201 ggtggcgctt gatcacctcg ttgttcggct cgatgcccac ggcacacttg tcggtaaagc
     1261 tcacgcaggc atcgccgagc aggcggccgc tttggagcac gttggcggca atgagcggct
     1321 tgaccacgtt gagttcgaag tggccctgca tgccacccac gctcaccgcc acgtcgttgc
     1381 cgatgacctg ggcgcacacc atggtgaggg cttcgggctg cgtgggattc accttgccgg
     1441 gcatgatgct gctgcccggt tcgttgtcgg ggatcacgat ctcgccgatg ccgctgcgcg
     1501 ggccactgct gaggatgcgg atgtcgttgg cgatcttcat caacgacacc gcgaggcgct
     1561 tgaaggcccc gctcagttcc accatcgcat cgtgagcggc caatgattcg aacttgttcg
     1621 gagcggtgat gaagggcagg ccggtgagtt cggcgatctt cttcgccacc agcacgctgt
     1681 agcccttggg cgcattgagg cccgtgccca cggccgtgcc gcccaacgcg agttcgcgta
     1741 tcatttccag cgcgttgttc actgcgcgca ggccattgtc cagttgctgc acatagccgc
     1801 tgaactcctg gcccagggtg agcggggtag cgtccatgaa gtgcgtgcga ccggtcttca
     1861 cgatcttatc aaaggccttc gccttcttgt tcaaggtgtc acgcagcttc ttcacgcccg
     1921 gcagggtcac ctccaccgtc tgcttgtagg cggcgatgtg catggc
//
LOCUS       DAOQOC010000594         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39327_length_1965_cov_3.092147, whole genome shotgun sequence.
ACCESSION   DAOQOC010000594 DAOQOC010000000
VERSION     DAOQOC010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39327_length_1965_cov_3.092147"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PL106_09720"
     CDS             complement(<1..792)
                     /locus_tag="PL106_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase subunit III"
                     /protein_id="HNA33385.1"
                     /translation="MSGEASKAIPNAVLWGGGRSPFSVSYGKIMMWFFLVSDALTFGG
                     LLVSYGFARHSMPPGESWPMGESVFRALPFVHGDFPLMYVAFMTAVLIFSSVTMVLAV
                     EAGHRMYKQGVIKWLFFTVIGGAIFVGSQVWEWGHFIHGSGGYVTMTNGDKYWVHNDT
                     HDTTDPAHAPGFHLVKADQVTHRYLQGEEGAPHIEGAEAQKLFAERVSYTHGASAPWA
                     SEGSIENEYGPAQYANFFFFITGFHGFHVFSGVVINLIVLIMVMQG"
     gene            complement(816..1547)
                     /locus_tag="PL106_09725"
     CDS             complement(816..1547)
                     /locus_tag="PL106_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33386.1"
                     /translation="MNSPAEDPIDEAAHRVRARRSLTYLFMVAIVMFFAALSSAYIVS
                     RGSADYWVTFRMPVDFWYSTAIIVVSSLSVQLALRAARHGDKRATATWLVATLVLGVI
                     FSVFQFKGWKEMSERRMNLVTDKVTMTAGVYGVDFAITHKGVPLERVDSLYYSQDDPG
                     HTAPLNADMADHWNVSSGYFHVLTFSHWLHLAGGLVVLMVLTVRALLGRYTAQAHTGV
                     WQGTMYWHFLTGVWIYLLLFIAAVH"
     gene            complement(1544..>1965)
                     /locus_tag="PL106_09730"
     CDS             complement(1544..>1965)
                     /locus_tag="PL106_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HNA33387.1"
                     /translation="PPMLGYVAATGQFGLGPGLLFAIQFMWQFPHYWAIAWVLHDDYK
                     LAGYHMLPSAGGRDGYSAWLTVLYTLFTVPVGMLPWFFGFTGPWAALVALLCGLWMLV
                     PAVRLLRTRSEKDARKLMFASFIYLPVTQIAFVIDKL"
BASE COUNT          416 a          600 c          604 g          345 t
ORIGIN      
        1 gccctgcatc accatgatga gcacgatgag gttgatcacc acaccgctga acacgtggaa
       61 gccatggaac cccgtgatga agaagaagaa gttggcgtac tgtgcggggc cgtactcatt
      121 ttcgatacta ccctcactgg cccatggagc cgaggccccg tgggtgtaac tcacgcgctc
      181 ggcgaaaagc ttctgggctt cggccccctc gatgtggggg gcgccttctt caccttgcag
      241 gtaacggtgg gtaacctggt cggccttcac caagtggaag cccggggcgt gggccgggtc
      301 ggtggtgtcg tgcgtgtcgt tgtggaccca gtacttgtcg ccattggtca tggtgacgta
      361 accaccgctc ccatggatga aatggcccca ctcccatacc tggctaccaa cgaaaatggc
      421 cccaccgatc acggtgaaga agagccactt gatcacgcct tgcttgtaca tgcggtgtcc
      481 ggcttcaacg gccagcacca tggtgaccga actgaaaatg agcaccgcgg tcatgaaggc
      541 cacgtacatc agggggaagt cgccgtgaac gaagggcagg gcgcggaaca cgctttcgcc
      601 catgggccaa gattcgcccg gaggcatgct gtggcgtgcg aatccataac tcaccagcag
      661 cccgccgaag gtgagtgcgt ccgacaccag gaagaaccac atcatgatct tcccgtagct
      721 cacgctgaac ggtgagcggc caccgcccca gaggacggcg ttcggaatgg ctttactggc
      781 ttctcctgac atggtccttt gttacgggcg caatgttagt gaacggcggc gatgaacaaa
      841 agcaagtaga tccatacacc ggtgaggaag tgccaataca tggttccctg ccataccccg
      901 gtgtgggcct gcgcggtgta tcggcccagc agggctctga ccgtcagtac catcaggacc
      961 accagtccac ctgccagatg gagccagtgg ctgaaggtga gaacatggaa gtatccgctg
     1021 ctcacattcc agtggtccgc catgtccgcg ttgagcggtg cggtgtgccc cgggtcgtcc
     1081 tgggagtagt acaggctgtc cacccgttcc agcggaaccc ccttgtgcgt aatggcgaaa
     1141 tccacgccgt acacccctgc cgtcatggtg accttgtcgg tcaccaggtt catccggcgt
     1201 tcggacatct ccttccagcc tttgaactgg aacaccgaga agatcacgcc gagcacgagg
     1261 gtcgcaacca gccaggtcgc cgtggcgcgc ttgtcgccgt ggcgtgccgc ccgcagcgcc
     1321 agttgcacgg aaaggctgct caccacgatg atggccgtgc tgtaccagaa gtccaccggc
     1381 atgcggaacg tgacccaata atccgcgctg ccacgactca cgatgtacgc gctgctgagc
     1441 gccgcgaaga acatcacgat cgccaccatg aacaggtagg tcaagctcct gcgggcgcgt
     1501 acccggtgcg ctgcttcatc gatcgggtcc tcggcagggc tgttcatagc ttgtcgatca
     1561 cgaaggcgat ctgcgtaacg ggcaggtaga tgaaactggc gaacatgagc ttgcgggcgt
     1621 ccttctcgct acgggtgcgc aagaggcgaa ccgcaggcac cagcatccag aggccgcaga
     1681 gcagggcgac caaggctgcc cacgggcctg taaagccgaa gaaccacggc agcatgccta
     1741 cgggcacggt gaacaaggtg tacagcacgg taagccacgc tgaataacca tcgcgcccgc
     1801 ccgcgctcgg cagcatgtgg tacccagcca gtttgtaatc gtcgtgcaac acccaggcga
     1861 tggcccagta gtgcgggaac tgccacatga actggatggc gaagagcagg ccgggcccca
     1921 ggccgaactg tccggtagcg gccacatatc ccagcatggg gggga
//
LOCUS       DAOQOC010000595         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39414_length_1963_cov_2.632600, whole genome shotgun sequence.
ACCESSION   DAOQOC010000595 DAOQOC010000000
VERSION     DAOQOC010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39414_length_1963_cov_2.632600"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PL106_09735"
     CDS             complement(<1..192)
                     /locus_tag="PL106_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33388.1"
                     /translation="MLHVHRLKIDKGERRIGLSIKAANYSPEQLKAEQALLDALKPGE
                     DLVALQHAFDAADEARQAED"
     gene            178..1095
                     /locus_tag="PL106_09740"
     CDS             178..1095
                     /locus_tag="PL106_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33389.1"
                     /translation="MYVEHVEKNFDLDAIRNSGMKLGYDAMYGAGQNVIPRILPKTTL
                     LHCDHNPSFRGQAPEPIHKNLIEFSELIKKQGLQCGLATDGDADRIGLYDSRGEFVDS
                     HHIILLLIHYLVKYKKFSGKVVVAVSTTPKVKKMCEHYGLEYQVTKIGFKWICGIMIN
                     EDVLLGGEESGGIAIKGHIPERDGIWMGLTIWEFMAKSGKTLEQLIDEVYGIVGPFKY
                     ERSDLHISEALKQQVLADCTSDKHKTFGPYAVRRLETIDGWKYHFDNDQWLMIRASGT
                     EPVLRTYAESSTLENARKILAACKTTIGA"
     gene            complement(1096..>1963)
                     /locus_tag="PL106_09745"
     CDS             complement(1096..>1963)
                     /locus_tag="PL106_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33390.1"
                     /translation="NTVYFGIGHRARSPIQRCRARLRWEVVFEGQPFSNSPITNSTAS
                     TATGAAWTMLPLAGTEIKELVMKFPFRMRYKWRVRAEYHPAKLIDGQRFSRWFYGYAS
                     AVGDIGILPIELLSFNGFSEGSSNELRWATASEHNSDRFEVQRSTDGESFAPLGSVPA
                     SGGSQSTVLYAFSDAFPPERIGYYRLRMIDLDGAEELSHTVTIARGDGLAGVYPNPVQ
                     DVARVVLGRDEAGASLSLLDAQGRVLIMGTAPGDGSPWELDMRTFSAGSYSVLLRDPS
                     GSVKDAIPLIKR"
BASE COUNT          407 a          572 c          633 g          351 t
ORIGIN      
        1 gtcttccgcc tggcgcgcct cgtcggcagc gtcgaaggcg tgttgcagcg ccaccaggtc
       61 ttcgccgggc ttcagggcgt cgagcagcgc ctgctcggcc ttgagttgtt cgggcgagta
      121 attggcggcc ttgatcgaga gaccgatgcg gcgctcgcct ttgtcgatct tgagacgatg
      181 tacgtggagc acgtggagaa gaacttcgac ctggacgcca tccggaacag cggcatgaag
      241 ctcggttacg atgccatgta cggtgcgggt cagaacgtga tcccgcgcat cctgccgaag
      301 accacgctgt tgcactgcga ccataacccg tcgttccgtg gtcaggcgcc cgagcctatc
      361 cacaagaacc tcatcgaatt cagcgaactg atcaagaagc agggcctaca gtgcggtctg
      421 gccacagatg gtgatgcgga tcgcatcggg ttgtacgaca gccgtggcga gttcgtggac
      481 agccaccaca tcatcctgct gctgatccac tacctggtga agtacaagaa attctcgggc
      541 aaggtggtgg tggcggtgag caccactccg aaggtcaaga agatgtgcga gcactacggg
      601 ttggagtacc aagtgaccaa gatcggattc aagtggatct gcggcatcat gatcaatgag
      661 gatgtgctcc tgggcgggga ggagagcggt ggcatcgcga tcaagggtca catacccgag
      721 cgcgacggga tctggatggg actcaccatt tgggaattca tggccaagag cggcaagacc
      781 ttggaacagc tcatcgacga ggtatacggc atagtgggcc cgttcaagta cgagcgcagc
      841 gatctgcaca tcagcgaggc attgaagcag caggtgctgg cggattgcac gagcgacaag
      901 cacaagacct tcggccctta cgcggtgcgc cgcctggaaa cgatcgatgg ctggaagtat
      961 cacttcgaca acgaccagtg gctcatgatc cgcgcgagcg gcacggagcc cgtgttgcgc
     1021 acctacgcgg aaagcagcac actggagaac gcccggaaga tcctggccgc ctgcaagacg
     1081 acgatcggcg cctgactatc gtttgatcag ggggatcgcg tccttcacgg agccggaagg
     1141 gtcccgcaag agcacggaat agctgcctgc gctgaaggtt cgcatgtcga gctcccaagg
     1201 ggacccatca ccgggtgcgg ttcccatgat caatacccgg ccctgggcat cgagcaagga
     1261 cagggaggcg cccgcttcat cccggccaag gaccacgcgc gcaacgtcct ggaccggatt
     1321 ggggtatacc ccggcgagtc catcgccccg ggcaatggtc acggtatggc tgagctcctc
     1381 cgccccgtcg aggtcgatca tgcgcaggcg gtagtagccg atgcgctccg gtgggaacgc
     1441 atcggagaac gcatagagca cggtgctctg gctcccgccg gaagcgggca cactgcccaa
     1501 tggtgcgaag ctctccccat ccgtggatcg ctgcacttcg aaccggtcgc tgttgtgctc
     1561 ggaggcggtg gcccaccgca actcgttgct gctcccttcg gagaatccgt tgaaactgag
     1621 cagttcgatg ggaaggatgc cgatgtcgcc cacggcactg gcatatccat agaaccaacg
     1681 actgaaacgc tggccatcga tcagcttggc ggggtggtac tcggcccgca cccgccattt
     1741 gtaacgcatt ctgaagggga acttcatcac cagttccttg atctcggtcc cggccagggg
     1801 aagcatggtc cacgcagcac ccgtggcggt ggaggcggtg ctattggtaa tgggcgagtt
     1861 gctgaacggc tggccttcga agaccacctc ccagcgcagt cgtgcacggc aacgctgaat
     1921 ggggctgcga gcgcgatgac cgatgccgaa atacacggtg ttc
//
LOCUS       DAOQOC010000596         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39475_length_1961_cov_4.322665, whole genome shotgun sequence.
ACCESSION   DAOQOC010000596 DAOQOC010000000
VERSION     DAOQOC010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39475_length_1961_cov_4.322665"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PL106_09750"
     CDS             <1..675
                     /locus_tag="PL106_09750"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNA33391.1"
                     /translation="ADGDDNVPSLSVTMVMATAAFVATAIVPMLPAWGSHHAAAAILV
                     LLLVSILDLMRRGVGDPARALALCISTIALIALPFASVTHLVHMHWHVFIGFMILLWT
                     NDTGAYLTGRAIGRHKLMPTVSPGKTWEGLLGGIGSTLGMAWLIALGWKELPLALWIA
                     SAVVISVSSTIGDLLESALKRAKGVKDSGSLLPGHGGILDRFDGFLLAAPAMLLLVKL
                     FERSGP"
     gene            702..1358
                     /locus_tag="PL106_09755"
     CDS             702..1358
                     /locus_tag="PL106_09755"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNA33392.1"
                     /translation="MTLHREGTPTILITIAVVCSGLFTAWTWMPAWAAWTFTVLLLLI
                     LCIVLWFFRVPARRITEDENAVISPCDGKVVAIELVEETEYFKDQRRQVSIFMSPLNV
                     HINFDPISGTTSYYKYHPGKYLVAWHPKSSTENERSTVVVKHPRHGEILFRQIAGALA
                     RRICTYSEVGGSARQGREFGFIKFGSRVDLFLPIDATMKVRIGDVVQGAISVIAEFKH
                     "
     gene            1497..1949
                     /locus_tag="PL106_09760"
     CDS             1497..1949
                     /locus_tag="PL106_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33393.1"
                     /translation="MPASRIDAPVDTPVVQAEDPDVRLVPLTGSGLRFDGVYRFDLGN
                     IHYYMRFFERGSAAFVGGSERNDGELADLLTIDVQSGWNSIHNCPVEQRNDSLFIRSV
                     GLKGVINYSGEVRANGDSVRFLRASEINGVRQIFRYAFVPDTMTTSRP"
BASE COUNT          330 a          621 c          600 g          410 t
ORIGIN      
        1 gccgatgggg atgataatgt tccgtccctt tcggtaacga tggtcatggc cacggccgcg
       61 ttcgtcgcca cggccatcgt gcccatgctg ccggcctggg ggagccatca tgccgcggcg
      121 gcgatcctcg tcctgctcct ggtatcgatc ctcgacctga tgcgccgtgg ggtgggggat
      181 cccgcacgtg ccctcgcgct gtgcatctcc accatcgcgc tcatcgccct gccgttcgcc
      241 tccgtcacgc atctggtaca catgcactgg catgttttta tcgggttcat gatcctgctg
      301 tggacgaatg ataccggggc ctatctcacg ggccgggcca tcggccgtca caaactcatg
      361 cccacggtaa gccccggcaa gacctgggag ggcctcctgg gcggtatcgg gtccaccttg
      421 ggcatggcct ggctgatcgc gctgggctgg aaagaactgc ctctggccct gtggatcgcg
      481 tccgccgtgg tcatctccgt ctcctccacc atcggcgacc tgctcgagag cgcgctcaaa
      541 cgggccaaag gggtgaagga cagtggatcc ctgctcccgg gccatggcgg catcctcgac
      601 cggttcgatg gtttcctgct ggcggccccg gccatgctgc tgctggtcaa gctgttcgag
      661 cggtccggcc cctgagcccg gatatcttcg ccaccccgat catgacgctc catcgcgaag
      721 gcaccccgac catcctgatc acgatcgcgg tcgtctgttc cggcctgttc accgcatgga
      781 catggatgcc cgcttgggcc gcgtggacct tcaccgtgtt gttgctcctg atcctctgca
      841 ttgtgctctg gttcttccgt gtgccggccc gcaggatcac cgaggacgag aacgcggtca
      901 tttcgccgtg tgatggcaag gtggtggcga tcgagttggt ggaggaaacg gagtatttca
      961 aggaccagcg tcggcaggtc tccattttca tgagcccgct caatgtgcat atcaacttcg
     1021 atccgatcag cggcacaacg agctattaca agtaccaccc gggcaagtat ctggtcgcat
     1081 ggcaccccaa gagcagcacc gagaacgagc gcagtaccgt ggtggtgaaa cacccgcggc
     1141 acggcgagat cctgttcaga cagatcgcgg gagcactggc ccgccgcatc tgcacataca
     1201 gcgaagtggg cggatccgcc cgccagggca gggagttcgg cttcatcaag ttcggctccc
     1261 gcgttgatct cttcctcccg atcgatgcca ccatgaaggt gcgcatcggc gatgtggtgc
     1321 agggagccat ttccgtgatc gctgagttca aacactgata gagcatgctt ttttccagga
     1381 tccttcgctc gcgggtggta tgccgatccg accaccgacc cttccgcggt ccgttccgga
     1441 cgaccttggt tctgggggtc ctctttggcg cagcctgtgt tgaccggccc gctgaagtgc
     1501 cggcctccag gatagatgcg ccggtcgata cgcccgtggt ccaagcggag gatcctgatg
     1561 tgcgacttgt cccgctcacc ggatcgggtc tgcgcttcga tggcgtctac cgcttcgacc
     1621 tcggcaacat ccattactac atgcgcttct tcgagcgcgg tagtgccgca ttcgtcgggg
     1681 gcagcgaaag gaacgatggc gaactggccg acctgctcac catcgacgtg cagagcggat
     1741 ggaacagcat ccataattgc ccggtggaac agcgtaacga cagcctgttc atccggtccg
     1801 tgggattgaa gggcgtgatc aattattcag gggaggtgcg tgccaacggc gattcggtgc
     1861 gtttcctgcg cgcgagtgag atcaacgggg tccggcagat cttccgctac gcattcgttc
     1921 cggatacgat gacgacgtcg cggccctgat caccccatca c
//
LOCUS       DAOQOC010000597         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39518_length_1960_cov_4.228871, whole genome shotgun sequence.
ACCESSION   DAOQOC010000597 DAOQOC010000000
VERSION     DAOQOC010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39518_length_1960_cov_4.228871"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="PL106_09765"
     CDS             complement(<1..1576)
                     /locus_tag="PL106_09765"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA33394.1"
                     /translation="MKKKLVDPNELAKASHMRPGDPRLALLTEVSGLGRLERLYNEID
                     LQPGDDFITRLFAHMELGLEVDGDDLAHIPATGGVVLVANHPYGAIDGLALIDVVRRV
                     RPEVKVMANFMLQQLEPLKDRFIGVNPFEQMKARSSFHGMRQAMEHLAAGGALGIFPA
                     GEVSSYRTELNAVADTRWKAPVIKLIKNAGVPVVPIWFDGANGVVFHMLGMIHPELRT
                     IALPGQMLRMRGKRVRLRIGKPILPTDHASLGVEEFTRYLRAKTYALGSGVQVKREFF
                     KPLRIPRRPKAIAQASHTGDLEKELGSIADLRLSSQGGFDLYLAPSERIPHMLYEIAR
                     QREIAFRAVGEGTNKSLDRDEFDLYYDHLFLWDTTAHKLAGAYRIGDGMRIMERYGKR
                     GFYTHTLFRMRKPMERVLGVSFELGRSFVPEEYRRHRLPLYMLWRGLLLHVISHPDHR
                     YLVGPVSISGTYSHFSRGLMIDFLRKHHFDHDLARYIRPRNRFIVRNEKADGQALLEA
                     SGEGLRKLDRIIADIEP"
     gene            1730..>1960
                     /locus_tag="PL106_09770"
     CDS             1730..>1960
                     /locus_tag="PL106_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33395.1"
                     /translation="MSFRAPVLLSTLVLFAYPLLAQGPYWGMTSSGGSFGVGTIYTIT
                     ESNVFTTKHNFLRFDGSTPKGDVVKASNGLYYG"
BASE COUNT          369 a          626 c          579 g          386 t
ORIGIN      
        1 cgggctcgat gtcggcgatg atccggtcca gcttgcggag gccctcgccg ctggcctcca
       61 gcaacgcctg accatcagcc ttctcgttgc gcacgatgaa tcggttgcgc ggccggatat
      121 atcgcgcaag atcatgatcg aaatggtgct tgcgcaggaa atcgatcatg agcccacggg
      181 agaaatggct gtacgtaccg ctgatgctga ccggcccgac caggtaacgg tggtccggat
      241 ggctgatcac atgaaggagc aggccgcgcc acaacatgta cagcgggagg cggtgacggc
      301 ggtactcctc gggcacgaag gagcggccga gctcgaagct cacgcccagg acacgctcca
      361 tcggcttccg catgcggaac aacgtatgcg tgtaaaagcc gcgtttgcca taccgctcca
      421 tgatccgcat gccatcgccg atgcggtatg cgccggccag cttgtgggcg gtggtgtccc
      481 acaggaacag gtggtcgtag tacagatcga actcgtcccg atcgagactc ttgttggtcc
      541 cctcgcccac ggcccggaag gcgatctccc gctggcgcgc gatctcgtac agcatgtgcg
      601 ggatccgttc gctgggtgcc aggtacaggt cgaagccacc ttgcgaactg aggcgcagat
      661 ccgcgatgga gcccaattcc ttctccagat ccccggtgtg cgaagcctgg gcgatggcct
      721 tgggacgacg cgggatgcgt aacggtttga agaactcgcg cttcacctgg acaccagaac
      781 ccaatgcgta ggtcttggcc cgcaggtatc gcgtgaactc ctccactccc agcgatgcgt
      841 gatcggtggg cagtatgggt ttgcctatgc gcaaccgcac acgctttcct cgcatccgga
      901 gcatttgacc gggcagggcg atggtgcgca actcggggtg gatcatgccg agcatgtgga
      961 agaccacgcc gttggcgcca tcgaaccaga tgggaaccac cggcaccccg gcgttcttga
     1021 tcagtttgat caccggcgcc ttccagcggg tgtcggccac cgcattcaat tcggtgcgat
     1081 agctgctcac ctcgccagca gggaatatgc ccagtgcgcc accggccgcc aggtgttcca
     1141 tggcctgacg cataccatga aagctcgacc gcgctttcat ctgctcgaac ggattcacgc
     1201 cgatgaaccg atccttgagc ggttcgagtt gctgcagcat gaagttggcc atcactttca
     1261 cctcgggccg cacgcgccgc accacatcga tgagcgccag cccgtcgatg gcaccgtacg
     1321 ggtgattggc cacgagcacc acaccgcccg tggccgggat gtgggccagg tcatcgccgt
     1381 ccacttccaa ccccaattcc atgtgggcga acaacctcgt gatgaagtca tctcctggtt
     1441 ggagatcgat ctcgttgtag agccgctcca accgccccag gccgctcacc tcggtaagca
     1501 gggccaaacg cgggtcgccc gggcgcatgt gactggcctt ggccagctca ttggggtcga
     1561 ccagtttctt tttcaaacgg cgggtcttcg gccaaatgta gcggggagta tcccagtgca
     1621 ggatgtccct ggttccggta caacgtacgc tcccagggcc cgaacccctt actttcaccc
     1681 gctccgaagg tgctgacacc gctccgtccc accccttacc cacataccga tgtcctttcg
     1741 tgctcccgtg ctcctttcca ccctcgtgct tttcgcatac cctcttctgg cgcaaggtcc
     1801 ctattggggc atgacctcca gcggtggcag ttttggtgtg ggcaccattt acacgatcac
     1861 cgaatcgaac gtgttcacca ccaagcacaa cttcctgcgt ttcgacggga gtacaccgaa
     1921 gggtgatgtc gtcaaagcga gcaatgggct gtattacggg
//
LOCUS       DAOQOC010000598         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39540_length_1960_cov_2.261417, whole genome shotgun sequence.
ACCESSION   DAOQOC010000598 DAOQOC010000000
VERSION     DAOQOC010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39540_length_1960_cov_2.261417"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PL106_09775"
     CDS             complement(<1..1518)
                     /locus_tag="PL106_09775"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNA33396.1"
                     /translation="MMRTLLTCLASTIALATLIAQPYDPLHPPDTYRNADNPYYWKNR
                     PPYPGYWQQDVHYRIKARLDENTDVADGSVILTYWNNSPDTLREVFFHLYQNAYNAGS
                     YLEKQERAEGGEPWDTTLQRGTEVSTLKMDGQETERVIDNTVMRVSLPRPIAPGGHTV
                     FEYTFRTHWQGGGRRMKLFNAWGYKHYDGTHWYPRISVYDKQRGWDTDQHLGHEFYGD
                     FGTYDVELDMPNNMIVEATGQLQEPELTLPPELRAKLDIGNFKDKPWNEKPSEPIPYD
                     AAVRRKWSYHAENVHDFAFTADPTYRIGQTEWNGVKCIAMAQEPHAAKWQNAADYCAK
                     VIRSHSTNFGMYAYPKMVVADARDGMEYPMLTLDSGNEPNYRGLFTHEIGHNWFFGMV
                     GNNETYRAMLDEGFTQFLTAFGLTDIDGDIMVEEPRRTRYEERFADPVSPREDEVYRG
                     YMIDAIRDDLPPINTHSDGFGYYTAQGRGGYRHVYMKTATMLYNLEYVLGDDLFRA"
     gene            complement(1581..>1960)
                     /locus_tag="PL106_09780"
     CDS             complement(1581..>1960)
                     /locus_tag="PL106_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNA33397.1"
                     /translation="EVRAPFMDPEVVEFVNALPGDLKRRGSNGKYLLKEVMRGKLPDN
                     IIDRPKKGFGIPLSDWLRNELRPLCEELLAPARITREGLFRSATIERLKQEHFSKRAN
                     HRKLLWALMVFELWHSGQGRDIT"
BASE COUNT          291 a          598 c          647 g          424 t
ORIGIN      
        1 ggcacggaac aggtcatcgc cgagcacgta ctccaggttg tagagcatgg tggcggtctt
       61 catgtacacg tggcggtagc cgccgcgccc ctgggccgtg tagtacccga acccgtcgct
      121 gtgggtgttg atggggggca ggtcgtcccg gatcgcatcg atcatgtatc cccggtacac
      181 ttcatcctcg cggggcgata ccgggtccgc gaaccgttcc tcgtaacgcg tgcggcgcgg
      241 ctcctccacc atgatgtccc catcgatgtc ggtgaggccg aaggcggtga ggaactgggt
      301 gaagccctcg tccagcatgg cgcggtaggt ctcgttgttg cccaccatgc cgaagaacca
      361 gttgtgcccg atctcgtgcg tgaagagccc gcggtagttc ggttcgttcc cgctgtccag
      421 cgtgagcatc ggatattcca tgccgtcgcg ggcatcggcc accaccattt tcgggtaggc
      481 gtacatgccg aagttcgtgc tgtgcgagcg gatcaccttc gcgcagtagt ccgcggcgtt
      541 ctgccacttg gcggcgtggg gctcctgggc catggcgatg catttcacgc cgttccattc
      601 ggtctgtccg atgcgatagg tcgggtcggc ggtgaaggcg aagtcatgca cgttctcggc
      661 gtggtagctc catttccggc gtaccgcggc atcgtacggg atgggctcgc tgggcttctc
      721 gttccagggc ttgtccttga agttgccgat gtcgagcttg gcccgcagct ccggtggcag
      781 cgttagttca ggttcttgca actggcccgt ggcctccacg atcatgttgt tcggcatgtc
      841 cagctccacg tcgtacgtac cgaaatcgcc gtagaactca tggccgaggt gctggtcggt
      901 gtcccatccg cgttgcttgt cgtacacgct gatgcgcggg taccagtgcg tgccgtcgta
      961 gtgcttgtag ccccatgcgt tgaagagctt catgcggcgg ccgccccctt gccagtgcgt
     1021 gcggaaggtg tactcgaata cggtgtgccc gcctggcgcg atcgggcgcg gcagggagac
     1081 gcgcatcacg gtgttgtcga tcacgcgttc ggtctcctgc ccgtccatct tcagcgtgct
     1141 cacctcggtg ccgcgctgca acgtcgtgtc ccaaggctcg ccgccctcgg cccgttcctg
     1201 cttctccagg tagctgccgg cattgtaggc gttctggtac aggtggaaga agacctcccg
     1261 cagcgtgtca ggcgaattgt tccagtatgt cagtataacg gaaccatcgg ccacgtcggt
     1321 gttctcgtcc aaccgggcct tgatccggta gtggacgtcc tgctgccagt agcccgggta
     1381 gggcggccgg ttcttccagt agtacgggtt gtccgcgttg cgataggtgt cgggcgggtg
     1441 gagcgggtcg tagggctggg cgatcaacgt ggccagcgcg atcgtggacg ccaggcaggt
     1501 caggagcgtg cgcatcatca gggggtgaat gtaccccgcg caaagttcct gatgtgccgg
     1561 gcgcgggccg ggccgaccgg tcaggtgatg tcccggccct gaccgctgtg ccagagttcg
     1621 aagaccatca gggcccacag gagcttgcga tggttcgcgc gcttgctgaa gtgctcctgc
     1681 ttcaagcgtt cgatcgtggc gctgcggaac agcccctccc gcgtgatccg tgctggcgcg
     1741 aggagttcct cgcacagcgg ccgcaattcg ttgcgcaacc agtcgctcaa ggggatgccg
     1801 aagcctttct tcggacggtc gatgatgttg tccggcagct tcccacgcat cacttccttc
     1861 aacaggtact tgccgttgga gccgcgtcgt ttcaggtccc cgggaagggc gttgacgaat
     1921 tccaccacct ccggatccat gaatggcgcc cgcacttcca
//
LOCUS       DAOQOC010000599         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39693_length_1957_cov_2.818086, whole genome shotgun sequence.
ACCESSION   DAOQOC010000599 DAOQOC010000000
VERSION     DAOQOC010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39693_length_1957_cov_2.818086"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1769)
                     /gene="dnaB"
                     /locus_tag="PL106_09785"
     CDS             complement(234..1769)
                     /gene="dnaB"
                     /locus_tag="PL106_09785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNA33398.1"
                     /translation="MADRRKRSTNSLIDTSLEAGKLPPQAPELEQAVLGAMMLERNAV
                     NEAIDILQPESFYVDAHKRVFGAIQELFRTDQPIDILTVTQQLKKAGELDIVGGPFYI
                     SQLTNKVASSANVQYHARIISQKHILRELIRISADTMRDAYDDTTDVFDLLDKSEQEM
                     YAITSGNLKRNYEPMSDLIQDAIANIENAKNKGDGVSGVPTGFLKMDELTGGWQRSDM
                     IIVAARPAMGKTAFVLSMARNIAVEHKKAVAIFSLEMSSTQLVTRLIASEAAISSEKL
                     RKGDLNDQEFAILHQHIARLTNAPIFIDDTPGLNIFELRAKCRRLKSQHNVELIIIDY
                     LQLMTAGSDGPRGGNREQEISQISRSIKSIAKELDVPIIALSQLSRAVESRTSDKRPI
                     LSDLRESGAIEQDADLVCFLYRAEYYKIFEDDHGSTLGVGECIVAKHRNGAIDTVRLR
                     FIPELAKFTDLEMGGAFTSIGSGTVGALGDGDPTLHITGGQRAITRPSRMNDDSNEEI
                     DPF"
BASE COUNT          333 a          564 c          618 g          442 t
ORIGIN      
        1 aacttcctcc tctgtagttg aggagccact caacggtcac atcacgctgg agtgtccaat
       61 acgcgatgcc gtaggccttc aagtggtctt tctgggtgcc gtccatgggg atcagcagct
      121 tggtggccca agcggaagcg ggcagcaggg agcaggcagc gaggatcagc cacagcttca
      181 gggtcggccg agctgcccgc tgccagattc cggcatcaga ccgcctgctg tcctcagaat
      241 ggatcgatct cttcgttgct gtcatcgttc atccggctgg ggcgcgtgat cgctcgctgt
      301 cctccggtga tgtgcaaagt tgggtcccca tcgcccaacg ccccgacggt accggaacca
      361 atacttgtga atgccccgcc catctcgagg tcggtgaact tggccagttc gggaatgaag
      421 cgcaagcgca ccgtgtcgat ggcaccattg cggtgcttgg ccacgatgca ttcgcccacg
      481 cccaaggtgc tgccatggtc atcctcgaag atcttgtagt actcggcccg gtacaggaag
      541 cagaccaggt cggcatcctg ctcaatggcg cccgattcgc gcaggtcgct gaggatgggt
      601 cgtttgtccg aggtgcgcga ttcgaccgca cggctcaact ggctcagggc gatgatgggg
      661 acatcgagtt ccttggcgat gctcttgatc gaacggctga tctggctgat ctcctgctcg
      721 cgattgccac ctcgcgggcc atcgctccct gcggtcatca gttgcaggta atcgatgatg
      781 atcagctcca cattgtgctg actcttcaag cggcggcact tggcgcgcaa ctcgaagatg
      841 ttgagaccgg gcgtatcgtc gatgaaaatg ggcgcgttgg tgagccgtgc gatatgctgg
      901 tgcaggatgg cgaactcctg gtcgttcagg tcgcccttgc gcaatttctc gctgctgatg
      961 gccgcctcac tggcgatgag tcgggtgacg agctgagtgc tgctcatctc caggctgaag
     1021 atcgctaccg cttttttgtg ttcaacggcg atgttgcggg ccatgctgag cacgaacgcc
     1081 gtcttcccca tggccggcct ggccgcaacg atgatcatgt cgctgcgctg ccagcccccg
     1141 gtgagttcgt ccattttgag gaagccggtg ggcacaccgc tcacgccatc gcccttgttc
     1201 ttggcgttct cgatgttggc gatggcgtcc tggatcaggt cgctcatcgg ctcgtagttg
     1261 cgcttgaggt tgccgctggt gatggcgtac atctcctgct cgctcttgtc cagcaggtcg
     1321 aacacgtccg tggtgtcgtc gtacgcatcg cgcatcgtat ccgcactgat gcggatgagt
     1381 tcgcgcagga tgtgtttctg actgatgatg cgcgcgtggt actgcacgtt ggcgctgctg
     1441 gccaccttgt tggtgagttg actgatgtag aaggggccgc ccacgatgtc cagctcgccc
     1501 gccttcttca actgctgcgt aacggtgagg atgtcgatcg gctgatccgt gcggaacagc
     1561 tcttggatgg caccgaagac gcgtttgtgg gcgtccacat aaaaactttc cggttgcagg
     1621 atgtcgatgg cctcgttcac cgcgttgcgc tccagcatca tggcccccag cacggcctgc
     1681 tcgagctcgg gtgcttgcgg cggaagctta ccggcttcca gcgatgtatc gatcagggag
     1741 ttcgtcgacc gtttgcggcg gtcagccatg cgggtggaga gcggatcggc cattggagga
     1801 tctatgtgga accggacaga aggtcccggt gcggggggcg cgaatttatc cgacgcacag
     1861 ggtgacaagc acgccatggg gcacccggtt atgacaacgt gtgaaccgat gcttgttgtt
     1921 cgatctgttg aaaagcctga acaccgcacc ggacctt
//
LOCUS       DAOQOC010000600         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39725_length_1956_cov_3.347712, whole genome shotgun sequence.
ACCESSION   DAOQOC010000600 DAOQOC010000000
VERSION     DAOQOC010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39725_length_1956_cov_3.347712"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PL106_09790"
     CDS             complement(<1..442)
                     /locus_tag="PL106_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNA33399.1"
                     /translation="MPSPLRVLTIIGARPQIIKAAAISRAIASHARGRIVETLLHTGQ
                     HYDANMSQVFFDELGIPPADINLNTGSGAHGAMTARMIEGIEQVLVRKEHDVVLLYGD
                     TNSTLAGAVAAAKLHVPVAHVEAGLRSFNKRMPEEVNRIVCDHCS"
     gene            complement(488..1198)
                     /gene="radC"
                     /locus_tag="PL106_09795"
     CDS             complement(488..1198)
                     /gene="radC"
                     /locus_tag="PL106_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNA33400.1"
                     /translation="MENLDESPVKLSIPEWSEADRPRERLLANGPKALSDAELVAILI
                     RSGTPKNSALDLARLILSKAGNDLHKLASLSVAELKKLKGMGEAKALSIVAALELGQR
                     RRNATVEQRPLIATSREAYEVLRPWVADLPQEQFWLLLLDRGNRLLDRVKVSEGGLHG
                     TVADPKWIFRKALEGGASSVILCHNHPSGQLRPSGEDITLTKKLVEGGRFLDIIVQDH
                     LIIAATGYFSFADNGQLG"
     gene            complement(1264..1518)
                     /gene="rpsT"
                     /locus_tag="PL106_09800"
     CDS             complement(1264..1518)
                     /gene="rpsT"
                     /locus_tag="PL106_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNA33401.1"
                     /translation="MANHKSALKRVRQTETRNERNRYQHKTTRNAVRDLRATTAKKEA
                     EKLLPEAESMLDKLAKKNVIHKNKAANLKSSLRKHVAKLK"
     gene            complement(1654..>1956)
                     /locus_tag="PL106_09805"
     CDS             complement(1654..>1956)
                     /locus_tag="PL106_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA33402.1"
                     /translation="QFLNVLLGQMSVVGPRPHPLKLNDQFRDIIDKYMVRHFVRPGIT
                     GWAQVNGFRGETRTPELMEKRVDLDVWYLENWSFKLDLKIIVKTVTNMLGKDERAY"
BASE COUNT          301 a          587 c          630 g          438 t
ORIGIN      
        1 tggagcaatg gtcgcacacg atgcgattca cctcctcggg catgcgcttg ttgaatgagc
       61 ggagcccggc ctccacgtgt gccacgggca catgcagttt ggccgcggcc acggccccgg
      121 ccagggtgct attggtgtcg ccgtagagca gcaccacgtc atgctccttg cgcacaagga
      181 cctgttcaat gccctcgatc atgcgggcgg tcatggcgcc atgcgcgccg ctaccggtat
      241 tcaggttgat atcggccgga ggtatgccca gttcgtcgaa gaacacctgg ctcatgttgg
      301 catcgtagtg ttggccggtg tgcagcagcg tttccacgat gcgccctcgg gcatgcgacg
      361 cgatggccct gctgatcgcg gcggccttga tgatctgcgg gcgcgcaccg atgatggtga
      421 gtaccctcaa tggtgatggc atgtacgtgg cgatcgcgtg ttttcgtgga tttgttgttg
      481 tggtgtccta tccgagctgt ccgttgtcgg caaagctgaa atagcccgtg gctgcaatga
      541 tcaggtggtc ctgcacaatg atgtccagga agcgcccgcc ctcgaccagt tttttcgtca
      601 gggtgatgtc ctcgccactc gggcgcagct ggccggaagg atgattgtgg cacaggatca
      661 ccgaactggc ccctccctcc agggctttac gaaagatcca cttagggtcg gccacggtgc
      721 cgtgcaggcc tccctcgctc accttcacac ggtccaacag acgattgccg cggtccaaca
      781 ggaggagcca gaactgctcc tgtggcaggt cggccaccca cgggcgcagc acttcgtagg
      841 cctcgcggct ggtggcgatc agggggcgct gttccaccgt ggcgttgcgg cgtcgctgac
      901 cgagttccag tgcggccacg atgctcaaag ccttggcctc gcccatgcct ttcagctttt
      961 tcagttcggc cacactcagg gaggccagct tgtgcaggtc gttcccggcc ttgctcagga
     1021 tcaggcgggc caggtccagc gcgctgttct tcggcgtgcc gctgcggatc aggatggcca
     1081 ccagttccgc atcgctcagg gccttggggc cgttcgcgag cagtcgctcc cgggggcggt
     1141 ccgcctcgga ccactcgggg atgctcagtt tgacagggct ttcgtccagg ttctccacgg
     1201 gccacaaagt agaacaggtc ccgcgtggga cctgtccgaa tatgagcatg ggtcggccgg
     1261 atcctacttc agtttggcca cgtgcttgcg caaactgctc ttcagattgg ccgccttgtt
     1321 cttgtggatc acgttcttct tggccagctt gtccagcatg ctttccgcct cgggcagcag
     1381 cttttcggcc tccttcttgg cggtggtggc ccgcaggtcg cgcacggcgt tgcgggtggt
     1441 tttgtgctgg tagcggttgc gctcgttgcg ggtctcggtc tggcggacgc gcttgagggc
     1501 gcttttgtgg ttggccatgc tatgaacgcc gaagcccggc ggaggcgttg tcttactgaa
     1561 aggggcgcga atatagtgct gcctgaccta tccacaatga tggtccacga aaaagccccg
     1621 ggtcatgggg ctcatccatg ggtccggtcc ggttcagtac gcccgttcgt ccttgcccag
     1681 catgttggtc accgttttca cgatgatctt caggtcgagc ttgaaggacc agttctccag
     1741 gtaccacacg tccaagtcca cgcgcttttc catcagttcg ggggtgcggg tttcgccgcg
     1801 gaagccgttc acttgggccc agccggtgat tccggggcgt acgaagtggc gcaccatgta
     1861 cttgtcgatg atgtcgcgga actggtcgtt cagcttcagg gggtgggggc gcgggcccac
     1921 cacactcatc tggcccagca gcacattgag gaactg
//
LOCUS       DAOQOC010000601         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39852_length_1953_cov_3.578504, whole genome shotgun sequence.
ACCESSION   DAOQOC010000601 DAOQOC010000000
VERSION     DAOQOC010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39852_length_1953_cov_3.578504"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1638)
                     /locus_tag="PL106_09810"
     CDS             complement(106..1638)
                     /locus_tag="PL106_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA33403.1"
                     /translation="MNNPNGPIKVLYVDDEQGNLLAFKAGFRRDFDVRTASSAQEALA
                     LLKDWHAHVVMSDQRMPGTNGAEFLAQVRAQRPDCIRIMVTGYSDIEAVVDAVNNGGI
                     HAYITKPWDPIDMKLRIEQAYEVYALRAAREQMFQRYRQVFDSAGDPIVIVDETGALL
                     DVNPATEKLLGIPRERILASNIRSFAPGSGELAGAMASHREGHTFMNIDITIVTPGGA
                     RLDCLMTATYQGRTANGKALFQAMIKDITDQKQEEKRLKELNDELDRRVAERTKQLMD
                     ALQDLGSFSYSVAHDLRSPLKSMRALTDHLNELAAYRNDVDLAEVSGRIHNSTSRLIA
                     LVDDLLRFARTNAKEVVRSEVDVRQAVISGLAGLDVSDRRLEVTMPDAGQAIVNVDEA
                     MFNVVLNNLLSNALKFTRTRPVARIAVTHEVVDGAHIVSVADNGVGFNAQQKDQVFGM
                     FKRLHKSEEFEGTGIGLAIVQRIVQKHGGDCWAEGGENEGATIHLRFPSSAASARLRM
                     VG"
     gene            complement(1635..>1953)
                     /locus_tag="PL106_09815"
     CDS             complement(1635..>1953)
                     /locus_tag="PL106_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA33404.1"
                     /translation="IQAIAARHDTADGRITVRTEQGECHTAITITDNGTGMAPEVCSR
                     IFEPFFTTKAVGEGTGLGLAIVHSIIEMHHGRIEVESAPDQGSTFRIIIPNTQPMATA
                     KRA"
BASE COUNT          346 a          603 c          580 g          424 t
ORIGIN      
        1 actgccggcg aacagaacgc tgtatgcgag gaagggggtg tgcttgaaca tgcttgttgc
       61 gtgtatgcgt ggccttggta cggggcgatc cgcctcaagg ttccatcaac ccaccatgcg
      121 caggcgtgcc gaggcggcgg aggaggggaa tcgcaggtgg atggtggccc cttcgttctc
      181 gcccccctcg gcccagcagt cgcctccgtg tttctgaaca atgcgctgca caatggccag
      241 accgatgcct gtgccctcga actcctcgga cttgtgcaac cgtttgaaca tgccgaagac
      301 ctggtccttc tgctgggcat tgaaccccac accattgtca gccacgctca cgatgtgggc
      361 gccatcgacc acttcatggg tgacagcgat gcgtgccacc gggcgtgtac gcgtgaattt
      421 cagcgcattg gagagcagat tgttcagcac cacgttgaac atggcctcgt caacgttcac
      481 aatggcctgg ccagcgtcgg gcatggtcac ttccaaccga cgatcgctca catcaagacc
      541 cgcaaggccg ctgatcaccg cttggcgcac atccacctct gaacggacca cctccttcgc
      601 attcgtccgg gcgaagcgca aaagatcatc gaccagggcg atcaaacgcg aggtgctgtt
      661 gtggatccgc ccgctcactt cggccagatc cacatcattg cggtacgcgg ccagctcgtt
      721 cagatgatcc gtcagggccc gcatgctctt gagcggggaa cgcaggtcgt gtgccacgga
      781 ataggagaat gatcccagat cctggagagc gtccatcaat tgcttggtcc gctcagccac
      841 ccgtcgatcg agttcgtcgt tcagttcctt caagcgcttt tcctcctgct tctgatcggt
      901 gatgtccttg atcatcgctt ggaacaacgc tttcccattc gcggtgcgcc cctggtaggt
      961 ggcggtcatc agacagtcca gtctggcacc tcccggggtg acgatcgtga tatcgatgtt
     1021 catgaaggta tggccctcac gatgggacgc catcgccccg gccagctcgc ccgatcccgg
     1081 tgcgaacgac ctgatgttcg aggccaggat ccgttcgcgg ggaatgccga ggagtttctc
     1141 ggtggccggg ttcacgtcca gcagggcccc ggtctcgtcc acgatcacga tgggatcacc
     1201 agcggaatcg aaaacctgcc gataacgctg gaacatctgc tcccgggcag cgcgcagcgc
     1261 gtatacctca tacgcctgtt caatgcgcaa tttcatatcg atgggatccc agggtttggt
     1321 gatgtacgca tggattccac cgttgttcac cgcatccacc accgcttcga tgtcggagta
     1381 cccggtgacc atgatgcgga tgcagtccgg ccgctgtgcg cgcacctgtg ccaggaattc
     1441 ggcgccgttg gtgcccggca tgcgctggtc actcatcacc acatgcgcgt gccagtcttt
     1501 caatagcgcc agggcctctt gtgcgctcga ggccgtacgc acgtcgaaat ccctccggaa
     1561 accggccttg aaggccagga ggttgccctg ctcgtcatcc acgtacagta ccttgatcgg
     1621 tccgttgggg ttgttcatgc gcgtttggcc gtggccatgg gttgtgtgtt cggtatgatg
     1681 atccggaagg tcgaaccttg gtccggggcg ctttcaacct cgatacgccc gtggtgcatc
     1741 tcgatgatac tgtgtacgat ggcgagtccc agacccgtgc cttcgcctac cgctttcgtt
     1801 gtgaagaagg gctcgaaaat gcgcgagcac acttccgggg ccatgcccgt gccgttgtcc
     1861 gtgatggtga tcgccgtgtg gcattcgcct tgttcggtgc gcaccgtgat ccggccatca
     1921 gccgtatcgt gccgggccgc tatggcctgg atg
//
LOCUS       DAOQOC010000602         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39887_length_1952_cov_4.489193, whole genome shotgun sequence.
ACCESSION   DAOQOC010000602 DAOQOC010000000
VERSION     DAOQOC010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39887_length_1952_cov_4.489193"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PL106_09820"
     CDS             complement(<1..515)
                     /locus_tag="PL106_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNA33405.1"
                     /translation="MEGTQRATDALAQDWILVVLLAALGLLGWVNIVSPKKWRLITRS
                     FFSFRLGRQSLRDELNLQDRTFIILLVAASVSVSLFAFQLAMRAGAVPGGPGEWGRFF
                     AVAVALLVVQVLALRLLGLLFQADGGLSEYLYTLLLLHVMLGICLLPLSASIAWPHRI
                     AWRSWALWTGLV"
     gene            635..1849
                     /locus_tag="PL106_09825"
     CDS             635..1849
                     /locus_tag="PL106_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA33406.1"
                     /translation="MSTTTATQKKAATDLYQHHDHYLVDELLTEEQKLIRDTARAHVS
                     KHLKPIIEERFEKAEFSKDIIPGLAGIGAFGPFVPEQYGGPGLDQISYGLIMQEIERC
                     DSGLRSLCSVQGSLAMYPIWKYGSEEQKKKWLPDMVQGKKIGCFGLTEPDFGSNPGGM
                     VTVFKDMGDHYLLNGAKMWISNSPFADLAIVWAKAENIEGRIHGLIVERGMEGFTTPT
                     THGKLSLRASPTGELVFDNVKVPKANLLPNKTGLGAPMGCLDSARYGIAWGTLGVAME
                     CYDTALRYSKERIQFGKPVGGFQLTQKKLAEMITEITKAQLLTWRLGVLRSEGRATTQ
                     QISMAKRNNVHLALTVAREARQILGGMGITNEYPIMRHMMNLESVVTYEGTHDIHLLI
                     TGADVTGIPAFK"
BASE COUNT          431 a          628 c          608 g          285 t
ORIGIN      
        1 acgagcccgg tccacaaggc ccagctgcgc cacgcgatcc ggtgcggcca ggcgatcgat
       61 gcggagagcg gcagcagaca gatgcccagc atcacgtgga gcagcagcaa ggtgtagagg
      121 tattcgctca gcccgccgtc cgcttggaac aacagcccca agaggcgcag agccagtact
      181 tggaccacca gcaaggccac ggccaccgcg aagaagcggc cccattcgcc cggcccaccg
      241 ggaaccgcgc ctgcccgcat ggccagctgg aacgcgaaga gtgataccga taccgaagcg
      301 gccacgagca gtatgatgaa cgtgcggtct tgcaggttca gctcatcgcg caggctctgg
      361 cgccccaaac ggaaggagaa gaacgagcgg gtgatcagcc gccacttctt gggcgaaaca
      421 atgttcaccc agcccagcag gcccagtgct gccaggagca ccaccaggat ccagtcctgg
      481 gccaaggcgt cggttgcgcg ctgggtccct tccatgcttc ggacaccata gccttgggcg
      541 gtggtggccg ccgcgaaagt gcgagtaccg gaggacttcg cgcgggaaac cgacgtccta
      601 cttttgcacg ccctgttgaa caggcacgca cccgatgagc accaccaccg ccactcagaa
      661 aaaagccgcc accgacctct accagcacca cgaccactac ctggtggacg agctgctcac
      721 cgaagaacag aaactgatcc gcgacacggc gcgggcccat gtgagcaagc atctgaagcc
      781 catcatcgag gagcgcttcg agaaggcgga gttcagcaag gacatcatcc ccggcttggc
      841 cggtatcggc gcattcgggc ccttcgtgcc cgagcagtac ggcggccccg ggctggacca
      901 gatcagctac ggcctcatca tgcaggagat cgagcggtgc gacagcggct tgcgcagctt
      961 gtgcagcgtg cagggctcgt tggccatgta ccccatctgg aaatacggta gcgaggagca
     1021 gaagaagaag tggctgcccg acatggtgca aggcaagaag atcggctgct tcggccttac
     1081 cgaacccgac ttcggcagca accccggcgg catggtcacc gtgttcaagg acatgggcga
     1141 ccactacctg ctgaacggtg ccaaaatgtg gatcagcaac tcgcccttcg ccgacctggc
     1201 catcgtgtgg gccaaggccg agaacatcga gggccgcatc catggattga tcgtggagcg
     1261 tggcatggaa ggcttcacca cgcccaccac gcacggcaaa ctctcgttgc gggccagtcc
     1321 caccggcgag ctcgtgttcg acaacgtgaa agtgcccaag gccaacctgc tgcccaacaa
     1381 aacgggcctg ggcgctccga tgggctgttt ggacagtgcg cgctacggca tcgcttgggg
     1441 cacgctcggc gtggccatgg agtgttacga cacggccctg cgctacagca aggagcgcat
     1501 ccagttcggc aaacccgtcg ggggcttcca gctcacgcag aagaagctgg ccgaaatgat
     1561 caccgagatc accaaggcac agctgctcac ctggcgcctc ggcgtattgc gcagcgaagg
     1621 ccgcgccacc acacaacaga tcagcatggc caagcgcaac aacgtgcatc tggcgctcac
     1681 cgtggcgcgt gaggcgaggc agatcctggg cggcatgggc atcacgaacg agtaccccat
     1741 catgcgccac atgatgaacc tcgaaagcgt ggtcacctac gagggcacgc acgacatcca
     1801 cctgctgatc acgggagcgg atgtgacggg gataccggcc ttcaagtagg ggagaggaac
     1861 tcaacgtccc cgtttgaacg acaaggtccc cgcatgatcc atgcgggggc cttgatcttg
     1921 gcgggagtgc cgagcctcaa tgcgtgtgct cc
//
LOCUS       DAOQOC010000603         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39918_length_1952_cov_2.638376, whole genome shotgun sequence.
ACCESSION   DAOQOC010000603 DAOQOC010000000
VERSION     DAOQOC010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39918_length_1952_cov_2.638376"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PL106_09830"
     CDS             <1..259
                     /locus_tag="PL106_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33407.1"
                     /translation="TYRASAVLDSIQQPMFDQGRKMVSSQWYDGGLHRGGSTFRYVDG
                     RLTMVSNMEKFKEGDHERWVIWGLKDGIFQPVNEKLVPLPE"
     gene            256..1056
                     /locus_tag="PL106_09835"
     CDS             256..1056
                     /locus_tag="PL106_09835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA33408.1"
                     /translation="MSAYEAAIEANRALWNARTEAHWNSAFYDVEGFLQGKGTLNRIE
                     LDLLGDVRGKDVLHLQCHFGQDTLSLARLGARVTGLDLSDGSIAKAQQLATQCGLPAR
                     FITGNVLERQPALEHAFDVVFSSYGTIGWLPVLDGWARNIAAYLKPGGLFVFAEFHPA
                     VWMFNNDFTAVAYSWFNRETIEELETGSYADRQASVKLPSFSWNHPLSDVLQNLLDAG
                     LRLEVFREFDRSPYDCFRHTVPTPDGQFMIRGMEGKLPMVYALRAVRT"
     gene            1181..>1952
                     /locus_tag="PL106_09840"
     CDS             1181..>1952
                     /locus_tag="PL106_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNA33409.1"
                     /translation="MGKHIERPLTWSGMLITLGIVFGDIGTSPLYVFKAIASGHVIEE
                     RLIYGAVSCIFWTLTLQTTFKYIWLTLQADNRGEGGVFSLYALVRRYGRWLYIPAIIG
                     ATTLLADGVIQPPISISAAVEGLEGVPAFADMIEPGGRSTVILVMVIIAGLFFFQRFG
                     TKVVGYAFGPIMFVWFTMLLVLGMVQIVEHPAVLKCVNPWYAYELLARYPGGFWLLGA
                     VFLCTTGAEALYNDMGHCGLRNIRWTWGFVKLALVANYM"
BASE COUNT          350 a          585 c          612 g          405 t
ORIGIN      
        1 aacctaccgg gcttcggccg tgctggacag catccagcag cccatgttcg accaagggcg
       61 gaaaatggtg agttcgcagt ggtacgatgg tgggctgcac cgcggaggaa gcaccttccg
      121 atatgtggat gggcggctga ccatggtgag caacatggaa aaattcaagg agggcgacca
      181 cgagcgctgg gtgatctggg gactgaagga cggtatcttt caacccgtga acgaaaagct
      241 ggtacccttg ccggaatgag tgcgtacgaa gcagcgatcg aagcgaaccg cgcgctgtgg
      301 aatgcgcgca ccgaggcgca ttggaactcg gccttttacg atgtggaagg cttcctgcag
      361 ggcaagggca cgttgaaccg cattgaactt gatctgctgg gcgatgtgcg tggcaaggac
      421 gtgctccacc tgcaatgcca tttcgggcag gataccctct cgctggcccg gctgggggcc
      481 cgggtcaccg gccttgatct gtccgacgga tccatcgcca aggcacagca actggccacg
      541 caatgcgggc tgccagcacg attcatcacc gggaatgtgc ttgagcggca acccgcgctg
      601 gagcatgcct tcgacgtggt cttcagttca tacggcacca tcggctggct gcccgtgctg
      661 gacggatggg cccgcaacat cgctgcctac ctgaaaccgg gcgggctttt cgtcttcgcg
      721 gagttccacc ccgctgtttg gatgttcaac aacgacttca ccgctgtggc gtactcctgg
      781 ttcaaccggg agacgatcga ggaactggaa accgggagct atgccgaccg acaggcatct
      841 gtcaagctcc cctctttctc gtggaaccac cccctgtcgg acgtgctcca gaacctcctg
      901 gatgccgggc tgcggttgga ggttttccgc gaattcgacc gctcgcccta cgactgcttc
      961 cgccacaccg tgcccacccc cgatggccag ttcatgatcc gaggtatgga ggggaagttg
     1021 ccgatggtgt acgccttgcg ggccgtgcgg acctgagtgc tcgtaacctc ccacttcgtt
     1081 atttcgcccc ctcgttccgg ccgccctctc ctgtccgggc aacctacggt agatcgaaat
     1141 agcagtttga acttcaaggg tcggcgtatt cacagaccgc atgggcaagc atattgaacg
     1201 gcccctgacg tggagcggta tgctcatcac gctggggatc gtcttcggcg acatcgggac
     1261 gagcccgtta tatgtgttca aggccattgc cagcgggcac gtgatcgaag agcgactcat
     1321 ctatggagcg gtcagttgca tcttctggac gctcaccctg cagaccacct tcaagtacat
     1381 ctggctaacg ctccaagcgg acaaccgggg tgagggcggt gtgttctccc tgtatgctct
     1441 ggtgcgacgg tatggccgct ggttgtacat cccggcgatc atcggggcca ccaccctgct
     1501 cgcggacggg gtgatccagc cgcccatctc catcagcgcg gccgtggagg ggctggaagg
     1561 tgtgccggcg ttcgcggaca tgatcgagcc gggcgggcgc tctaccgtga tcctggtcat
     1621 ggtgatcatc gcaggtctgt tcttcttcca gcggttcggc accaaggtcg tgggctatgc
     1681 cttcgggccg atcatgttcg tctggttcac catgctgctc gtcctcggca tggtccagat
     1741 cgtggagcac ccggccgtgc tcaaatgcgt gaatccgtgg tacgcctacg agctcctcgc
     1801 gcgctacccc ggcggtttct ggctgctggg tgcggtgttc ctgtgtacca cgggtgccga
     1861 agccctttac aacgatatgg gacattgcgg cctgcggaac atccgatgga cctggggctt
     1921 cgtgaagctc gccctggtgg ccaactacat gg
//
LOCUS       DAOQOC010000604         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_39934_length_1951_cov_4.515823, whole genome shotgun sequence.
ACCESSION   DAOQOC010000604 DAOQOC010000000
VERSION     DAOQOC010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39934_length_1951_cov_4.515823"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PL106_09845"
     CDS             <1..927
                     /locus_tag="PL106_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA33410.1"
                     /translation="LLFAALIIGTASAQQLPQLSQYTSNDYLYCHAVAGSRPWFEIRS
                     THRNQWVGVQDAPRTFMLSATSPVGARMGLGGFIYTDHVGPTRRTGAQVSYTYHLRLN
                     DRIRLGMGLSFGLQQFLIDGSKISFRDNHDPVMDNQLRGSLVPDAAFSLLLHHEKWWV
                     GVMVPQLIESRIAFYDENDQSMSTLSRHYYGMAGYRFALGDDFRIEPSVLVKYVDPVP
                     PKFDLTATLRYKEQVWLGATYRTNDAMAVMIGYWLKKTFQVGYAYDITTTNLRRYSNG
                     THEVMLALTIGKKSPVRSTPPASGATPPPSAP"
     gene            1146..>1951
                     /locus_tag="PL106_09850"
     CDS             1146..>1951
                     /locus_tag="PL106_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33411.1"
                     /translation="MAVFDKPLNILHAYWLAAALLIGGGGKLAAQSFPMADGAQWETC
                     TGTFYDSGGSGGDYAANENLTATLCPAGGPGAGPFSSITFATWAVAPGTADQLAIYNG
                     DDIGDPLLATGSGANPLLGQTFTSTDPSGCLTFVWTSDATVQGAGWVAQINTGPDAGT
                     SADTTVCSTAAPFGLFSVLNGDPDAGGQWTLNSNLVSGTFDPAVGPGGVYTYTVPGVP
                     PCVNATATVTVTLVSAANAGFDNTIEVCTTESPFSMRSRLLGTPQPGGDWT"
BASE COUNT          357 a          605 c          617 g          372 t
ORIGIN      
        1 ctcctgttcg cggcgctgat catcggcacc gccagtgcgc aacagctccc gcaattgagc
       61 cagtacacga gcaacgatta cctgtactgc cacgcggtgg cgggaagcag gccctggttc
      121 gagatccgga gcacccatcg caaccaatgg gtgggcgttc aggacgcgcc gcgcaccttc
      181 atgctcagtg cgacctcgcc ggtgggtgcc aggatgggcc tgggcggatt tatttatacc
      241 gaccatgtgg gtccgacacg gcggaccggg gcacaggtga gctacacgta ccacctccgg
      301 ttgaatgaca ggatccgctt gggcatgggg ctctccttcg ggttgcagca gttcctgatc
      361 gatgggtcga agatctcctt ccgcgataac cacgacccgg tcatggacaa ccagttgcgc
      421 ggaagcctcg tgcccgacgc ggccttctcc ctgttgctgc accacgagaa gtggtgggtg
      481 ggcgtcatgg tgccacagct gatcgagagc cgcatcgcgt tctacgatga gaacgaccag
      541 agcatgagca ccctgtcgcg gcactactac ggcatggccg gttaccgctt cgcgttgggg
      601 gatgacttcc ggatcgaacc ttccgtactt gtcaaatacg tggatccggt acctccgaag
      661 ttcgacctta cggccacgtt gcgctacaag gagcaggtgt ggttgggtgc cacataccgc
      721 accaacgatg ctatggcggt gatgatcggt tactggttga agaagacctt ccaggtcgga
      781 tacgcgtacg acatcaccac caccaatctc aggcgttaca gcaacggcac gcatgaggtg
      841 atgctggcgt tgaccatcgg gaagaagtcc ccggtgcggt caacgccacc cgcgagcggt
      901 gcaacgccac ccccatcggc accgtaaggg acctgtccat acttaggcgg cgggtgaggt
      961 atgggaacac ccgtagggcg tgaacatcgc cccgttccgt acgggcactc caggatcgtc
     1021 gatgaaaacc ggttattcgt cgatcgtgcc gcttggcgcg cgccgcgcag gcatccaatt
     1081 tctcgccggt tcgtctatgc cgacgttact ttgcacgtcc tcttaccgga cctgaatcgc
     1141 ttaccgtggc cgttttcgac aagccgctga acattctcca cgcttactgg cttgctgctg
     1201 cactcctgat cggtggtggg ggtaagctgg cggcacagag cttcccgatg gcggatggtg
     1261 cgcaatggga aacgtgtacg ggtacctttt acgacagtgg tggatccggg ggtgattacg
     1321 cggcgaacga gaacctgacc gctacgctgt gcccggccgg aggtcccggg gcaggtccgt
     1381 tcagcagcat cacctttgcg acctgggcgg tggcaccggg cacagcggac cagttggcga
     1441 tctacaatgg ggacgatatc ggcgacccct tgctggctac cggctctggt gcgaacccgc
     1501 tgctgggcca gaccttcacc tccaccgacc cgagcggctg cctcaccttc gtgtggacga
     1561 gtgacgccac ggtgcagggt gcgggctggg tcgcgcagat caataccggt ccggatgccg
     1621 gcaccagcgc ggacaccacg gtgtgcagca cggccgcgcc cttcgggctt ttctccgtac
     1681 tcaacgggga tcctgacgcg ggtggacagt ggaccctgaa cagcaacctg gtcagcggga
     1741 ccttcgaccc tgcggtcggg cccggaggtg tgtacaccta cacggttccc ggggtgccac
     1801 cttgcgtgaa cgccacagcc actgtaacgg tgaccctcgt gagtgcggcg aatgccggtt
     1861 tcgacaacac catcgaggtc tgcaccacgg agtccccgtt cagcatgcgg tcgcgattgc
     1921 tgggcacccc acagcctggt ggggattgga c
//
LOCUS       DAOQOC010000605         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40034_length_1949_cov_2.982049, whole genome shotgun sequence.
ACCESSION   DAOQOC010000605 DAOQOC010000000
VERSION     DAOQOC010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40034_length_1949_cov_2.982049"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="purD"
                     /locus_tag="PL106_09855"
     CDS             complement(<1..1004)
                     /gene="purD"
                     /locus_tag="PL106_09855"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNA33412.1"
                     /translation="MNVLLIGSGGREHALAWKLSKSTLLDELYVTPGNPGTVRHGRNV
                     QLDLGDQKAVRNFILEKKIRMVVVGPEAPLVDGLHDRIAADPKLAGVTVIGPKAEGAR
                     LEGSKEFAKEFMLRHNIPTAAFRVFGKGQLKEAVAYLDLLGNGPYVIKADGLAQGKGV
                     VIAQGRKEAEQALKEMLGGRFGAASERVVIEQFLKGIELSAFLVTDGNSFKMLPAAKD
                     YKRIGDGDTGPNTGGMGAVSPAPFADRDFMQKVHDRVAAPTIRGLKADGIPYQGFLFM
                     GLMNVNGEPYVIEYNVRLGDPETQAILPRLQSDLMDLFEGIASGTLSDTDRVNLQSEF
                     A"
     gene            1121..1921
                     /locus_tag="PL106_09860"
     CDS             1121..1921
                     /locus_tag="PL106_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA33413.1"
                     /translation="MEQPRLRALLVDDEELARENRRLLLADGCPEVDVVGIADGLNSA
                     RQRIAELDPDILFLDIRMPSGTEGLDLLDDAPGEGPGTDPRFMVIFVTAFKDHAVQAF
                     HNNAVDYVLKPIDPEELRAAVDKALRRWERMHGAPEEGLRYRRELGDAVRTIKRHEAE
                     PRIAVDHAKGYKLFEPRTMVHLEADGNCTVLHFTDGSRFLDTRTLKVYECMLDPGLFF
                     RTHRSHIVNIDQVREYLREDGHWAVMKDGSRVPIARERVQAFVESTRG"
BASE COUNT          324 a          673 c          596 g          356 t
ORIGIN      
        1 gcgaactcgc tctgcaggtt gacccggtcg gtgtcgctga gggtgccgct ggcgatgcct
       61 tcgaagaggt ccatcaggtc gctctgcagc cgtggaagga tggcctgtgt ctcggggtcg
      121 cccaagcgca cgttgtactc gatcacgtac ggttcgccgt tcacattcat caggcccatg
      181 aacaggaaac cctggtacgg gatgccgtcg gctttcagcc cccggatggt gggtgccgcc
      241 acccggtcgt gcaccttctg catgaagtcc cggtcggcga acggggcggg gctcaccgct
      301 cccatgccac cggtattggg gcccgtatca ccgtcgccta tccgcttgta atccttggcc
      361 gcagggagca ttttgaagga gttcccatcg gtcaccagga aggcactcag ctcgattccc
      421 ttgaggaact gctcgatcac cacgcgctca ctggccgcac cgaaccggcc gcccagcatc
      481 tccttcaggg cctgctcggc ctccttgcgg ccttgtgcga tcaccacacc cttgccttgg
      541 gccaggccgt cggccttgat cacgtacggg ccgttgccca gcaggtccag gtaggccacg
      601 gcctccttca gctggccctt gccgaaaacg cggaaggccg ccgtggggat gttgtgccgc
      661 aacatgaact ccttggcgaa ctccttgctg ccctccaggc gcgcaccttc ggccttgggg
      721 ccgatgaccg tgacgcccgc cagcttgggg tcggcggcga tgcgatcgtg caggccatcc
      781 accaacgggg cttccggccc caccaccacc atgcggatct tcttctccag gatgaagttc
      841 cgcaccgcct tctggtcccc caggtccagt tgcacattgc ggccatggcg caccgtgccc
      901 gggttgccgg gggtcacgta caactcgtca agcagggtgc tcttgctcag tttccatgcc
      961 aacgcgtgct cgcgtccgcc gctgccgatc aggagtacat tcatgcgttc tcgctctttg
     1021 gcgcaaaggt ggccttccga acaccctccc gaggccacaa gttatgcacg gatcaacagg
     1081 gtacctgccc gaccacggcc gcagggtatt ttcggcccgc atggaacagc cacgcctgcg
     1141 cgccctgctg gtggacgacg aggagctcgc acgcgagaac cgccgcctgc tgctggccga
     1201 tggatgtccc gaagtggacg tggtggggat cgccgatggc ctcaacagcg cccgtcaacg
     1261 gatcgccgaa ctggatccgg acatcctctt cctcgacatc cgcatgccca gcggtaccga
     1321 ggggctcgat ctgctcgatg acgcacccgg cgaaggaccc ggtaccgatc cccgcttcat
     1381 ggtcattttc gtcaccgcct tcaaggatca tgcggtgcag gccttccaca acaacgcggt
     1441 ggactacgtg ctgaagccca tcgaccccga ggagttgcgc gccgcggtgg acaaggccct
     1501 gcgtcgctgg gaacgcatgc acggcgcgcc cgaggaaggc ctgcgctacc gccgggaact
     1561 cggtgatgcg gtgcgcacca tcaagcggca cgaggccgaa ccgcgcatcg ccgtggacca
     1621 tgccaagggt tacaagctct tcgaaccgcg caccatggtc catctggaag ccgacggcaa
     1681 ctgcacggtg ctgcatttca ccgacgggtc ccgattcctg gatacgcgta ccttgaaggt
     1741 gtacgagtgc atgctcgatc ccggactctt tttccgcacc caccgttcgc atatcgtgaa
     1801 catcgaccag gtgcgcgagt acctccgcga ggatggccac tgggccgtga tgaaggacgg
     1861 gtcccgcgtg cccatcgccc gcgagcgggt gcaggccttc gtggagtcca cccgcggata
     1921 gcccgcccga cacggtccgt cggccccgg
//
LOCUS       DAOQOC010000606         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40044_length_1949_cov_2.761880, whole genome shotgun sequence.
ACCESSION   DAOQOC010000606 DAOQOC010000000
VERSION     DAOQOC010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40044_length_1949_cov_2.761880"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PL106_09865"
     CDS             <1..1256
                     /locus_tag="PL106_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33414.1"
                     /translation="HDQSPDCRPECVEDENNIDITPTGGFYNLAEVEGALPFNPAQHC
                     NGGILDAIHKSVQEHRADYAAKYAQLASAALACAGQLDNGERVEVLEAIKAEPAWPSH
                     QLRGYLLARSPLSEEAIITAINRAEPMDPWHLTQVLIANSKLSDPIWAALDHSEALSP
                     FFYQMVLDHQEDPSIRQILMEELALRAYEKDTEQRLLVLALHEDSTYTGKLDTLLSIL
                     STDTLGLGRTAAYQLALAHGRGTEAAALAAAMAGDARFEQLLGLGALQLGLDPGWSQA
                     GHNEVMALQDMAASGKKLGRGAAWGILYALGATDSLPTGLLPVEYRSARANETDASTA
                     ERPVVGAYPNPAKDRLMITWPAEVDAGTLEVTDARGRSVLSQALGSRTGFVELDVRGW
                     ADGLYLARVLRDGQVVGETKCAIAR"
     gene            1253..>1949
                     /locus_tag="PL106_09870"
     CDS             1253..>1949
                     /locus_tag="PL106_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33415.1"
                     /translation="MRRAIAIAVLLAGSIYAPCQSTFIVQFDSVQSGHDASGRSIMET
                     ENGLIVFAWQHSDDGTGRMRGAVYKLAPDGTFLSRYEIGTGELTGSYFGRWDPVTRMN
                     TGGFASMFVRSNGYGYNIDMIRFDDNGDVLSFNDVMDSPPEDSIVVGSRQLRQTGDGG
                     FVLCGAFSLPDGYAKALLVKLDSTGAVQWQRTYDAAGQSYDAISVAQYVDGGYVLAGY
                     RLPQGLNGQGFLIR"
BASE COUNT          379 a          572 c          645 g          353 t
ORIGIN      
        1 cccacgacca aagccccgat tgccgaccgg agtgcgtgga ggatgagaac aacattgaca
       61 ttacaccgac aggtggattt tacaatctgg ctgaagtaga gggagcattg ccgttcaatc
      121 cggcccaaca ttgcaatggt ggcatcctgg acgccattca caaaagcgtt caggagcacc
      181 gggcggacta cgcggccaag tacgcgcaat tggccagtgc ggccctggcc tgtgccgggc
      241 agctggacaa tggcgaacgg gtggaggtgt tggaagcgat aaaggccgag cccgcttggc
      301 ccagccacca gttgcgcggc tacctgctgg cccgcagccc gctgagcgag gaggccatca
      361 tcacggccat caaccgggcc gagcccatgg acccctggca cctgacccag gtgctcatcg
      421 ccaacagcaa actctcggac cccatctggg cggccttgga ccacagcgaa gcgctgtcgc
      481 ccttcttcta ccagatggtg ctcgaccacc aggaggatcc cagcatccgc cagatcctga
      541 tggaggagct ggccctgcgc gcctacgaga aggacaccga gcaacgcctg ctggtgctgg
      601 ccctgcacga ggacagtacg tacaccggca agttggatac cctcctctcc atcctcagca
      661 cggacacact ggggctgggc cgtacggcgg cctatcaact ggcgctggcc cacgggcgtg
      721 gcaccgaggc cgccgcactg gcagcggcca tggccgggga tgcgcgtttt gagcagctgc
      781 tcggcctcgg cgcgttgcaa ttgggcctcg atcccggctg gtcgcaggcc gggcacaacg
      841 aggtgatggc cctgcaggac atggccgcca gcggcaaaaa gctgggccgg ggcgcggcgt
      901 ggggcatcct gtacgcgctg ggcgccaccg acagcctgcc caccggcctg ctgcccgttg
      961 agtaccgctc ggcacgggcg aatgaaacgg acgccagcac agcggagcgg cccgtggtag
     1021 gcgcctaccc caacccggcg aaggatcggc tgatgatcac gtggcccgct gaggtggacg
     1081 cgggtacctt ggaggtgacc gatgcccggg gccgttcggt gctttctcaa gcacttggat
     1141 cacgtaccgg ttttgtggaa ttggacgtgc gcggctgggc agatggactg tacttggcgc
     1201 gtgtgctacg cgatggccag gtggtgggtg agaccaaatg cgcgatcgcc cgatgaggcg
     1261 ggcgatcgcc atcgcggtgt tgctggcagg gagcatatat gctccctgcc agagcacctt
     1321 catcgtgcaa ttcgacagcg ttcaatcagg ccacgatgcc agtggccggt cgatcatgga
     1381 gaccgagaat ggactcatcg tattcgcgtg gcaacattct gatgatggta ctgggcggat
     1441 gcggggcgct gtgtataagc tggcgcccga tggcacattc ttaagccgat acgagatcgg
     1501 tacgggcgaa ttgactggat cctatttcgg gcggtgggat cctgtgaccc gaatgaatac
     1561 cggaggcttc gcttcgatgt tcgtacgcag taatgggtat ggatacaaca tcgatatgat
     1621 ccggttcgac gacaacgggg atgtgctttc gttcaatgat gtgatggact ctccgccaga
     1681 agattcgatc gtggtcggat cccggcaatt gcggcaaacc ggggatggag gattcgtcct
     1741 ttgcggtgcg ttttcccttc cagatggata tgccaaagcc ctgctggtga aattggacag
     1801 caccggcgca gtccaatggc agcggacgta cgatgcggcg ggccagagct acgatgccat
     1861 cagcgtggcg caatacgtgg acggcggcta tgttctggcc ggctaccgct tgccgcaggg
     1921 cttgaacgga caagggttct tgatccgta
//
LOCUS       DAOQOC010000607         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40074_length_1948_cov_3.458003, whole genome shotgun sequence.
ACCESSION   DAOQOC010000607 DAOQOC010000000
VERSION     DAOQOC010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40074_length_1948_cov_3.458003"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PL106_09875"
     CDS             <1..240
                     /locus_tag="PL106_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33416.1"
                     /translation="HMAEHDGALAHIDLGGPRAIRLELPWVHALVEHDIRTNHARRIH
                     QFRWSWWLRDHTRSLLRTFGIRTRRERTAHAARTA"
     gene            221..1513
                     /locus_tag="PL106_09880"
     CDS             221..1513
                     /locus_tag="PL106_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA33417.1"
                     /translation="MLHVLLEPASPRARYVVRHVFERMLGWPFVLVTSREELRSVAGP
                     KLLYGPTPEKGAYHVPACGWLADPGPAGPRPTISGNGRDLVLFPVSSGHDVFAAIFHL
                     IALEEEYAITHLDEHGRIRADELTLVERDAHGFPIADRWALNLASELRTAFPSLPQPQ
                     RRYSHVLTVDMDNGLKYTGRSWQRAMGASLKDLIGGTPGRLLERWKVRTGNAPDPYAS
                     FVDRLSEVRDKVDRMISFVLTRGEGRYDHAARIDHAAFAALLKQLVGFSELGLHPSYA
                     SSREERLILHERGLLERASGAPIHISRQHFLRWRMPDTFRALIATGIQEDHSMGFSDR
                     SGFRAGTCTPFPWYDVERDEETTLMLHPFAVMDSALANRTGDGLLKSISAMEQVCDAV
                     REVNGTFVSVWHDRFLSGHEEFKGGPEAMRELVMYAKA"
     gene            1510..>1948
                     /locus_tag="PL106_09885"
     CDS             1510..>1948
                     /locus_tag="PL106_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33418.1"
                     /translation="MIRYVRHSGIDMDAWDARLERCANKLWYGLSTTLHAAAPGWDAL
                     MDEETGMQMALPWRRKWGITYAYQPFLLQQLGPFAPEPRPGDTARFVAALPPHIRFAD
                     IYLCPGTSGPSLPEQQNISFSLRKPIDELRSCYSENHRRTLKKT"
BASE COUNT          373 a          629 c          604 g          342 t
ORIGIN      
        1 cacatggcgg agcacgatgg cgcattggcg cacatcgacc tgggcggtcc acgcgcgatc
       61 cgattggaac tgccctgggt ccacgccttg gtcgaacacg acatccggac gaaccacgca
      121 cgccggatcc accagttccg atggtcctgg tggttgaggg atcataccag aagcctgctt
      181 cgtacgttcg gcatccgcac gcgccgggag cgcaccgccc atgctgcacg tactgcttga
      241 acccgcatcg cctcgagccc gatacgtggt gcgccacgtg ttcgagcgca tgttgggctg
      301 gccattcgta ctggtcacgt cgcgtgagga actccggtcg gtggcaggcc cgaaactgct
      361 ctatggcccc accccggaga aaggggccta tcacgttccc gcttgtggct ggctggccga
      421 tccaggacct gccggtccac gaccgaccat cagcggcaac ggccgcgacc ttgtgctctt
      481 tccggtcagc tcagggcacg atgttttcgc cgcgatcttc cacctcatcg cgcttgaaga
      541 ggagtatgcg atcactcacc tcgatgagca cgggcggatc cgggcagatg agctcacact
      601 cgtggaacgc gatgcgcacg ggttccccat cgctgaccgg tgggcattga acctcgcgag
      661 cgaactccgg acggcattcc cttcgcttcc ccaacctcag cgcaggtaca gccatgtgct
      721 caccgtggac atggacaacg gcttgaagta cacgggccgg tcatggcagc gggccatggg
      781 ggcttcgctc aaagacctga tcggcggtac gccaggccgg ttgctcgaac gttggaaagt
      841 gcgcacgggc aacgcccctg atccatacgc gtcattcgtc gatcggcttt ccgaagtacg
      901 cgacaaggtg gaccgcatga tcagcttcgt gctcacccgg ggcgaagggc gatacgacca
      961 tgctgcgcgc atcgatcatg cggcgttcgc cgccctgctc aaacaactgg tggggttctc
     1021 cgaactgggg ctgcatccct cgtacgcgag cagtcgcgaa gaacgactca tcctgcacga
     1081 acgtgggctc ctggagcggg caagcggtgc gccgatccac atttccaggc agcatttttt
     1141 acgttggcgc atgccggaca ccttccgtgc gctcatcgcg accgggatcc aggaggacca
     1201 ctccatgggc ttcagcgacc ggtccgggtt ccgggccggc acgtgtacgc ctttcccctg
     1261 gtacgacgtg gaacgggatg aggagaccac gttgatgctc catcccttcg ccgtgatgga
     1321 cagcgcactg gcgaaccgga ccggtgatgg actgctgaag tcgataagcg cgatggaaca
     1381 ggtgtgcgat gcggtacggg aagtgaacgg gacgttcgtg agcgtgtggc acgaccggtt
     1441 cctcagtggg cacgaggaat tcaaaggcgg ccccgaagcc atgcgcgaac ttgtaatgta
     1501 cgccaaggca tgatccgtta tgtacggcat agcgggatcg acatggacgc gtgggacgcc
     1561 cggctcgagc gctgcgcgaa caagctctgg tacgggctga gtacaacact ccacgccgcc
     1621 gcacccggct gggatgcgct catggacgag gagaccggca tgcagatggc actgccctgg
     1681 cgccgaaaat ggggcatcac gtacgcctac caacctttcc tgctccagca gctcggtccg
     1741 ttcgcaccgg aaccacgacc cggagacaca gcgcgtttcg tggccgcgct gccaccccac
     1801 atccggttcg cggacatcta cctctgcccg ggaacaagcg gcccgtcctt accggaacag
     1861 cagaacatct ccttcagcct gcgcaagccg atcgatgagc tccggtcgtg ctatagcgag
     1921 aatcaccggc gcacgctcaa gaagaccg
//
LOCUS       DAOQOC010000608         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40085_length_1948_cov_2.865293, whole genome shotgun sequence.
ACCESSION   DAOQOC010000608 DAOQOC010000000
VERSION     DAOQOC010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40085_length_1948_cov_2.865293"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(702..>1948)
                     /locus_tag="PL106_09890"
     CDS             complement(702..>1948)
                     /locus_tag="PL106_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33419.1"
                     /translation="LMLNDDKVIMAKDNFQRVTSHSTSAKINYLVASETVRPTELKDQ
                     DIKDMSAFLKTNATNPRVLITGVKIDAYASPEGEIHMNEGLADKRAATARKWLQGELA
                     KNKYAKAKNDSLYTLVPRGEDWEGFRAATQASDFKDKDLVLRVLEMYPDVEKRESEIK
                     NMAATYNELRENILPQLRRSEIVVNYDKTGYTDAELAQLSRTSPDSLNVEELLKAADN
                     AKADVNEQLRIYKECERVHAGDYRASNNIGAIYYAQNKMTEAEAQFQKANSITNNPIS
                     TNNLAAITRQKGDRKKAQELLKKSLSAGPEVKYNLGLIDVQNGEYSSANGNFSGTNSV
                     NAALAKVLGGDAAAAQGILQQASDKDSAVGHYVMAIIAARQNNCDLMRTELAAAVAKD
                     PKLADKAKKDLEFRGCKDSLGL"
BASE COUNT          321 a          564 c          602 g          461 t
ORIGIN      
        1 tacaaggtgc agggtggcca caagtaccgc acccgtatcc gcaggcagaa gatcgtgttc
       61 tgatcctgcc tccgatcagc gttgaagaag ctgtccttcc gggcggcttt ttccttgagg
      121 accacagcca tccgtgttcg tccttggaat ggggagtccc ttcccgcgta ccgctgtgcg
      181 ggggatcaaa acggtgatcg taattcacga ggggcactgg ggcctgtgtg gagcaggtgg
      241 tgagcggagg tttgtacaga tccatgacct gggagcgcgc gtggcaccga aatgggccaa
      301 gcgcagcctt gtccaaggga aacggaatac cgggtgcatc agaaatcagt ccgtgtggcc
      361 ggggatccgg ctggtggtcg tttgacgcat gtatggccat acatacgtca gcaccagtgt
      421 gtcgttggac gtgcatccgg gagcttgaat gatcgcgcgg acgtgccatt caatatgctg
      481 gatcgcccaa catatgtatg gccatacata tgttgatggt gtgcgaatgg ggcaccccac
      541 gagttggcgc gtatgccact gtcgcggccc agccatgggc gtgtacgcgt caggtccgcg
      601 tgcggatccc tccggcctat cgaacaccat gccgacccaa aacgaagaac ccctggcaca
      661 aggccaaggg ttcttccaaa atgagaagcg cgaccggctg ctcagagccc gaggctgtcc
      721 ttgcaaccgc ggaactccag gtccttcttg gccttgtcgg ccagtttcgg atccttggcc
      781 acggcggcgg ccagttcggt gcgcatcaga tcgcaattgt tctggcgggc ggcaatgatc
      841 gccatcacat agtgtcccac ggccgagtcc ttgtcggagg cctgttgcag gatcccctgt
      901 gctgctgcgg catcaccacc gagcactttg gccagtgcgg cgttcacgct gttggtgccg
      961 gagaagttgc cgttggcact gctgtactcg ccgttctgca catcgatcag gcccaggttg
     1021 tacttcacct ccggacccgc gctcaggctc ttcttcagca gttcctgggc cttcttgcga
     1081 tcgcctttct ggcgggtgat ggcggccagg ttgttggtgc tgatggggtt gttcgtgatg
     1141 ctgttggcct tctggaactg ggcctcagcc tcggtcatct tgttctgggc gtagtagatc
     1201 gcgccgatgt tgttgctggc gcggtagtca ccggcatgca cgcgctcgca ctccttgtag
     1261 atccgcaatt gctcgttcac atcggccttg gcgttgtcgg cggccttcag caactcttcc
     1321 acgttcaggc tgtcgggcga cgtacggctc aactgggcca actccgcatc ggtgtagccg
     1381 gtcttatcgt agttcaccac gatctccgaa cggcgcagct ggggcagaat gttctcgcga
     1441 agctcgttgt acgtggcggc catgttcttg atctcgcttt cgcgcttctc cacatcgggg
     1501 tacatctcca gcacccgcag taccaggtcc ttgtccttga aatcgctggc ctgggtggcg
     1561 gcgcggaagc cttcccaatc ctcgccgcga ggaaccaggg tatagagcga gtcgttcttg
     1621 gccttggcgt acttgttctt ggcgagctcg ccttgcagcc acttgcgggc ggtggccgca
     1681 cgcttgtcgg ccaggccttc gttcatgtgg atctcccctt cgggtgatgc gtacgcatcg
     1741 atcttgaccc cggtgatcaa cacacgcggg ttggtcgcgt tggtcttcaa gaaggcggac
     1801 atgtccttga tgtcctgatc cttcaattcc gtcggacgca cggtttcaga ggccaccagg
     1861 taattgatct tggcactggt gctgtggctg gtcacgcgtt ggaagttgtc cttggccatg
     1921 atcaccttgt cgtcgttgag catcaggt
//
LOCUS       DAOQOC010000609         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40106_length_1947_cov_4.693446, whole genome shotgun sequence.
ACCESSION   DAOQOC010000609 DAOQOC010000000
VERSION     DAOQOC010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40106_length_1947_cov_4.693446"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..531)
                     /locus_tag="PL106_09895"
     CDS             complement(85..531)
                     /locus_tag="PL106_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain protein"
                     /protein_id="HNA33420.1"
                     /translation="MRIYIDTSVFGGCFDKEFEEWSVGLFDQIRAGLHRAVISDISEA
                     ELSVAPEQVRQLLKELKSKHLDVMRLSPDAEALATAYIKERIVTKKSELDTQHIAIAT
                     IAQVDLLVSWNFKHIVNYNRIRLYNAVNLKLGYRLLEIRSPRDLTA"
     gene            complement(535..>1947)
                     /locus_tag="PL106_09900"
     CDS             complement(535..>1947)
                     /locus_tag="PL106_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA33421.1"
                     /translation="ISEDLVKQAFIDQRTEGWADFRKEVLAVDIAAMERETGVDRNLV
                     REAAIAYASSPAAMSFHGLGVTEHYQGTFTVMQIADLAMMTGNIGRRGVGVNPLRGQN
                     NVQGAADMGAQPHQGAGYLDLASSAVNKQYEAHYGVPMPTRIGWKIPEMFNAAIEGKL
                     KALWLMGEDVVQTDPNTQKVIKAMESLDLLVVQEIFMTETARYADVILPAATFLEKSG
                     TFTNGERRIQRVNEVVPPLEGTKSDGQIVCDVMERFAQRTGTRSGNAKTVYHPDWVLE
                     EISRIVPFFAGVKWEELGENGKQWPVKPDGTDTRILHTDTFKRGLGRFIFKPWVESPE
                     VRANGKDYPYIITTNRDLEHYNSGTMTRRTGNGEIHTEDLLLINPADAQQNGIADGDM
                     VCVESPRGKVDIKARVTDEVKPGILSSTFHFPEVMLNIITSDIHCTEALCPEYKVVTC
                     RIRKARKGQMRKAGEVVVNE"
BASE COUNT          310 a          609 c          579 g          449 t
ORIGIN      
        1 catgccgttc gcgatatatg gcgtcgcgga tctcgcgcac catcttcacg ctatcgtaac
       61 ctttcttggg tttggtcgtg gtgctcatgc agtaaggtct ctgggactgc gaatctccag
      121 cagccggtag ccgagcttca aatttacagc attgtacagc cgaatgcgat tgtagttcac
      181 gatgtgcttg aagttccaac tcaccagcag gtccacctgt gcgatggtgg cgatggcgat
      241 gtgctgggta tccaattcgg atttcttggt gacgatgcgt tccttgatat acgcggtggc
      301 gagcgcttcg gcatccgggg agaggcgcat cacatcaagg tgcttcgact tcagctcctt
      361 cagcaattgg cgcacctgct ccggtgctac agagagttca gcttcactga tatcggaaat
      421 gaccgcccgg tgcagaccgg ccctgatctg atcgaagagc cccactgacc attcctcgaa
      481 ctccttatcg aagcagccac cgaagaccga ggtatcgatg tagatcctca tcgactattc
      541 attcaccacc acctcccccg ccttccgcat ctgtcccttc ctcgccttgc ggatccggca
      601 cgtcaccacc ttgtactccg ggcatagtgc ctcggtgcag tgtatgtcgc tcgtgatgat
      661 gttgagcatc acctccggga agtggaaggt gctgctgagg atgccgggtt tcacctcatc
      721 ggtcacacgg gccttgatgt ccaccttgcc gcgcggactt tccacgcaca ccatgtcgcc
      781 atcagcgatg ccgttctgct gggcgtcggc gggattgatc agcagcaggt cctcggtgtg
      841 gatctcgccg ttgccggtgc ggcgcgtcat ggtgccgctg ttgtagtgct ccagatccct
      901 gttggtggtg atgatatacg ggtagtcctt gccgttcgca cgcacctcgg ggctctccac
      961 ccagggcttg aagatgaagc ggcccagtcc gcgtttgaaa gtgtcggtgt gcaggatccg
     1021 cgtatcggtc ccgtcgggct tcacgggcca ttgcttgccg ttctcgccca gttcctccca
     1081 cttcaccccg gcgaagaagg gcacgatgcg gctgatctcc tccagcaccc agtccgggtg
     1141 gtacacggtc ttggcgttgc cactgcgggt gccggtgcgc tgggcgaagc gctccatcac
     1201 gtcgcacacg atctggccgt cgctcttcgt gccttccagc ggtggcacta cttcgttcac
     1261 gcgttggatc cggcgttcac cgttggtgaa ggtgccgctc ttctccagga aggtggcggc
     1321 cggcaggatc acgtcggcat accgtgccgt ttcggtcatg aagatctcct gcaccaccag
     1381 caggtccagg ctttccatgg ccttgatcac cttctgcgtg ttggggtcgg tctgcaccac
     1441 atcctcgccc atcagccaca gggccttcag cttgccctca atggcggcgt tgaacatctc
     1501 ggggatcttc caaccgatgc gtgtgggcat gggcacgccg tagtgcgctt cgtactgctt
     1561 gttcacggcg ctgctggcga ggtccaaata gccggcgccc tggtgcggtt gtgcgcccat
     1621 gtcggcggcg ccctgtacgt tgttctgtcc gcgcagcggg ttcacgccaa caccgcgccg
     1681 gccgatgttg ccggtcatca tggccaggtc ggcgatctgc atcacggtga aggtgccctg
     1741 gtagtgctcg gtaacgccca ggccgtggaa gctcatggcg gctggcgagc tggcgtaggc
     1801 gatggccgcc tcacgcacga ggttccgatc cacgccggtc tcgcgttcca tggcagcgat
     1861 gtccacggca agcacttctt tgcggaaatc ggcccagccc tcggtgcgct gatcgatgaa
     1921 cgcctgcttc accaggtcct cgctgat
//
LOCUS       DAOQOC010000610         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40123_length_1947_cov_3.077696, whole genome shotgun sequence.
ACCESSION   DAOQOC010000610 DAOQOC010000000
VERSION     DAOQOC010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40123_length_1947_cov_3.077696"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PL106_09905"
     CDS             <1..412
                     /locus_tag="PL106_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA33422.1"
                     /translation="KGQAVDVMAPGTLVALGDAHWTTQILQALISNASKFSPRGSSIS
                     INAGATADGSAITVSDKGTGFTGSDLVDVFTRYAILSSRTTAGEPQARGTLARAKQWA
                     RAQGGHLNVESEGPGQGARFTLTLPPAQSLLGIS"
     gene            complement(552..1502)
                     /locus_tag="PL106_09910"
     CDS             complement(552..1502)
                     /locus_tag="PL106_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNA33423.1"
                     /translation="MNTIAGPVRALKLGVFYDGSYFSHVSNYYNYVHPHHRRLHIGGL
                     HDFIRHTVAQKEGITPNLCHIIDAHFFRGRFSAKDANEKTNQLYYDRVFDDVLMYNNV
                     QTHYLPVKDLMGRKREKGIDVLMALETYELCMLKRYDVVALIASDGDHTPLVRKLHAL
                     GCKTMLLGWDFEYTDELTGEHQTTRTSTDLWNNVTYPLEMSDVVEEGLRNDDEVITDM
                     FVAKDANLPARDPNADPDRPLVSISDYEDTERHTSTIMSLHKGYGFIRFPENNLFFLH
                     DDLIDIDFNTLAVDDMMEFSVAMNSKGQRVAKRIKRAETV"
     gene            complement(1655..>1947)
                     /locus_tag="PL106_09915"
     CDS             complement(1655..>1947)
                     /locus_tag="PL106_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNA33424.1"
                     /translation="LKLMKCGGLDRAKAMADKARQLGMQVMLGSMSESSLGCSAMAQL
                     ASEADIVDLDGPWLIQNDPFKGIDMLDGRLRLPDGPGMGAQLVGDLAFTAAH"
BASE COUNT          377 a          564 c          590 g          416 t
ORIGIN      
        1 gaaaggccag gccgtggacg tgatggcccc gggaaccctg gtcgccttgg gcgatgcgca
       61 ttggaccacg cagatcctgc aagcgctcat cagcaacgcg agcaagttct ccccacgtgg
      121 ttcgtccatt tccatcaacg ctggggccac tgcggatggt tcagccatca cggtctcgga
      181 caaaggcacc ggctttacgg gatcggacct cgtggatgtg ttcacccggt acgcgatcct
      241 ctcttcccgg accaccgctg gcgaaccaca agcgcgcggc accctcgcac gcgccaagca
      301 atgggcccgc gcccagggtg gtcacctgaa cgtcgagagt gaaggccccg gccagggagc
      361 ccggttcacg ctgaccctcc ctcctgctca gagcctgttg gggatctctt gaacgaagga
      421 ctccgaggct atttttcgtt caggcgcagc cggaaaatta tcgcgtagcg gtgcctacgg
      481 gaccattttc cggcgaagca tgggcggaaa agagaccgga ggatgagttt gaagagatcc
      541 cgaacaggtt cttagaccgt ttcggcgcgt ttgatccgct tggccacgcg ctggcctttg
      601 ctgttcatcg ccacgctgaa ctccatcatg tcgtccacgg ccagggtgtt gaagtcgata
      661 tcgatcaggt catcgtgcag gaagaacagg ttgttctccg ggaagcggat gaagccatag
      721 cccttgtgca ggctcatgat ggtactggtg tggcgctcgg tatcctcata gtcgctgatg
      781 ctgacgagcg gacggtcggg atcggcgttc ggatcgcgcg caggcaggtt cgcatccttg
      841 gccacgaaca tgtcggtgat gacctcgtcg tcgttccgca gaccttcctc caccacgtca
      901 ctcatctcca atggataggt gacgttgttc cagaggtcag tgctggtccg cgtggtctgg
      961 tgctcacccg tgagttcgtc ggtgtattcg aagtcccagc cgagcagcat ggtcttgcag
     1021 cccaacgcgt gcagtttgcg cacgagcggg gtgtgatcac cgtcgctggc gatcagggcg
     1081 accacgtcgt accgcttcag catgcacaac tcgtaggtct ccagtgccat cagcacatcg
     1141 atccccttct cccgtttgcg gcccatgagg tccttcacgg ggaggtagtg ggtctgcacg
     1201 ttgttgtaca tgagcacgtc gtcgaacacg cggtcgtaat acaactggtt ggtcttctca
     1261 ttggcatcct tcgcactgaa gcgtccgcgg aagaaatgcg catcgatgat gtggcagaga
     1321 ttgggcgtga tgccttcctt ctgtgccacg gtatggcgga tgaaatcgtg caatccgccg
     1381 atatgcaacc tgcgatggtg cggatgcaca tagttgtaat agttgctcac atggctgaag
     1441 tacgagccat cgtagaagac gcccagctta agggcacgaa cgggtccggc aatagtgttc
     1501 atgggaaatg gatgtaagtg gccgtatatg tatgggttgg agcattacgg cggggcaagt
     1561 tatcctcaac ggtcacgctt tgcttcaagg gacaccgatg aacgcccgaa ggatcagcga
     1621 ccagggcttg gttttttcag gtccgatgga tggatcaatg ggccgcggtg aacgccagat
     1681 cgcccacgag ctgcgcgccc atgccggggc cgtcgggcag ccgcagccga ccatccaaca
     1741 tatcgatccc tttgaacgga tcattctgga tgagccaggg cccatccaga tcgacgatat
     1801 cggcctccga tgcgagttgg gccatggccg aacatccgag cgagctttca ctcatgctgc
     1861 cgagcatcac ctgcatgccg agttgccggg ccttatcggc catggccttg gcccggtcca
     1921 atcctccgca tttcatcagc ttgaggt
//
LOCUS       DAOQOC010000611         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40245_length_1944_cov_2.542615, whole genome shotgun sequence.
ACCESSION   DAOQOC010000611 DAOQOC010000000
VERSION     DAOQOC010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40245_length_1944_cov_2.542615"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PL106_09920"
     CDS             complement(<1..1547)
                     /locus_tag="PL106_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNA33425.1"
                     /translation="MMHFRPITLGLLLSAASVPAQKDLTNQEIWFSPTFGVERVGGLN
                     SMNDGLRYTALDDDGSGSAINIYDYRSGEKTGTLVSAKDLGGLPLEGYALSGDEQRVM
                     LETAIEPQYRYSYYAHHFVYDLRTRTLRPLCDTTKSKQRLATFSPDGSRAAFVRDNDL
                     YVTDLATMKETRITTDGEWNKVINGATDWVYEEEFTLVQGYQWNSTGTKLVYLRSDES
                     DVKEFNVVLYRDQLYPTDYRFKYPKAGEKNSTVSLHVHDLTTGRTTGIQLGTKETDIY
                     LPRFGFTQDPNTVWFMRMNRLQNEKLILTMNTDGTTTEPVLVYRETSPTYIEVTDDLY
                     FAEDGSGFILTNETTGWNQIHWCPLIPKRTVDGTTFTRPITNGAYDVLSVKGIDRAGK
                     RVIFTASMKDPMHSEVYAIGLNGKGLKELSPPGGSNDPEFSKGFKYFINVRSTANEPP
                     VTTLHDGNGQLVKTLKDNAKLKSRLAEYQLPKIEFIKAKLDNGTEVNAWVLKPRNFDS
                     RQQHPVF"
     gene            complement(1648..>1944)
                     /locus_tag="PL106_09925"
     CDS             complement(1648..>1944)
                     /locus_tag="PL106_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33426.1"
                     /translation="RYPENQLVEVIYGVNMVASCQFLYWLWNYGSAVPGLLIGEVPVE
                     LKREIHTLFLFAPSVYLIAIGISFVEPLWSFYFYLITPLVYLIPTRLDQYLPKR"
BASE COUNT          334 a          552 c          621 g          437 t
ORIGIN      
        1 atgaataccg ggtgctgctg tcggctgtcg aaattcctgg gcttcagcac ccaggcattc
       61 acctcggtgc cattgtccag cttcgccttg atgaattcga tcttcggcag ctggtattcg
      121 gccaggcggc tcttcagttt ggcgttgtcc ttcaaggtct tcaccaactg tccgttccca
      181 tcgtgcagcg tggtcacggg cggttcgttg gcggtgctgc gcacattgat gaaatacttg
      241 aagcccttgc tgaactccgg atcgttggat ccgccgggcg gggagagttc cttcaggccc
      301 ttgccgttca ggccgatggc gtacacctcg ctgtgcatcg ggtccttcat gctggcggtg
      361 aagatcacgc gcttgccagc ccggtcgatg cccttcacgg agagcacgtc atacgcgccg
      421 ttggtgatgg gccgggtgaa tgtggtcccg tccacggtcc gcttgggaat gagcgggcac
      481 cagtggatct ggttccagcc ggtggtctcg ttggtgagga tgaagccgct gccgtcctca
      541 gcgaagtaca ggtcgtccgt cacctcgatg tacgtggggc tggtctccct gtaaacgagt
      601 accggttccg tcgtggtccc atcggtgttc atggtcagga tgagcttctc gttctgcagg
      661 cggttcatgc gcatgaacca gacggtgttc gggtcctgcg tgaagccgaa gcggggcagg
      721 tatatgtccg tctccttggt gcccagttgt atgcccgtgg tccggcccgt ggtgaggtcg
      781 tggacatgca aggacaccgt gctgttcttc tcgccggcct tggggtactt gaagcggtag
      841 tcggtaggat agagctgatc gcgataaagc accacgttga actccttcac atcgctctca
      901 tcgctgcgga gatacacgag tttggtgccg gtactgttcc actggtagcc ctgcaccagg
      961 gtgaactcct cctcgtaaac ccagtcggtg gcaccgttga tcaccttgtt ccattcgcca
     1021 tcggtggtga tccgggtctc cttcatggtg gccaggtcgg tcacgtacag gtcgttgtcg
     1081 cgcacgaaag ccgccctgga cccatcgggg ctgaaggtgg ccaggcgctg tttgcttttc
     1141 gtggtgtcgc aaagcggccg cagggtccgg gtgcgcaggt cgtacacgaa gtggtgcgcg
     1201 tagtaactgt accggtattg gggttcgatc gcggtttcca gcatcacgcg ttgctcgtca
     1261 ccgctgagcg cgtagccttc cagcggaagc ccgccaaggt ccttggcgct gaccagggtg
     1321 ccggtctttt cgccggaccg gtaatcgtag atgttgatcg cggacccgct gccatcatcg
     1381 tccagcgcgg tgtagcgcag gccgtcgttc atactgttga ggccgcccac gcgttccaca
     1441 ccgaaggtcg ggctgaacca gatctcctgg ttggtgaggt ccttctgtgc tggaacggaa
     1501 gcggcgctga gcagcaggcc cagggtgatc ggtcggaagt gcatcatgtg ttcggctaca
     1561 atggcgggcc aagatagcct tgtgccgttc gcaaggacgg tgccgttcgt gtacggccca
     1621 accgttcacg cccccgtatc gccgacctta tcgcttcgga aggtactggt ccaggcgggt
     1681 gggaatgagg tacaccagag gcgtgatcaa atagaagtag aagctccata ggggttcaac
     1741 gaagctgatg ccgatcgcga tcaagtacac ggatggggcg aacaggaaca aggtatggat
     1801 ctcccgcttg agttcaaccg ggacctcgcc gatcaggagg cccggaacgg cgctgccgta
     1861 gttccacagc cagtagagga actggcagga cgccaccatg ttcacaccgt agatcacctc
     1921 cacgagctga ttctccggat agcg
//
LOCUS       DAOQOC010000612         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40313_length_1942_cov_2.906730, whole genome shotgun sequence.
ACCESSION   DAOQOC010000612 DAOQOC010000000
VERSION     DAOQOC010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40313_length_1942_cov_2.906730"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PL106_09930"
     CDS             complement(<1..1066)
                     /locus_tag="PL106_09930"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNA33427.1"
                     /translation="MRSLAILGSTGSIGTQALQVVREQPDHFTVELLVAGNNADLLIA
                     QAREFKPNAVVIADKARYPAVRDALQPLDIKVFAGAESIEQAVTMEGIHTVLTAMVGY
                     AGLRPTLAAIGAGKTIALANKETLVVAGELVTKAAREKGVNIYPVDSEHSAIFQCMAG
                     EWDNPIEQVILTASGGPFRGRSRAELEHVTAAEALKHPNWNMGAKVTIDSASLMNKGL
                     EAIEAKWLFSLTPEQIDIVVHPQSIVHSLVRFRDGSLKAQLGLPDMKLPIQYALGYPE
                     RLATTWPRFDFMAYPQLTFSQPDREAFPNLQLALDAMTRGGNIPCALNAANEVAVAGF
                     LEGRIGFLHMSDIIAGTLARS"
     gene            complement(1360..>1942)
                     /locus_tag="PL106_09935"
     CDS             complement(1360..>1942)
                     /locus_tag="PL106_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HNA33428.1"
                     /translation="LLRALDLPLAAPSANPFGYVSPTTAQHVADQLGEKIPYILDGGP
                     CTVGVESTIIGWEDGLPVLYRPGGLALERIEEVVGTVRPHVKQVLPVAPGMIESHYAP
                     RKPVLVGDVDRLLREHADERCGVIAFRNVHDAWQCETLAPNGDISEAARRLFGVLREL
                     DASAADLIIAERFPEEGLGRAINDRLRRAAAKR"
BASE COUNT          324 a          630 c          621 g          367 t
ORIGIN      
        1 tgctgcgcgc aagggtgccg gcgatgatgt cgctcatgtg caggaagccg atgcgcccct
       61 ccaagaaccc ggccacggcc acttcgttcg ccgcgttgag tgcgcaaggg atgttgccgc
      121 ctctggtcat ggcgtccagt gcgagttgca gattggggaa ggcctccctg tcaggttggc
      181 tgaaggtgag ttgcggatag gccatgaagt cgaaccgggg ccatgtggtg gccagtcgtt
      241 cggggtagcc cagcgcatac tggatgggca acttcatatc aggcaggccc agttgtgctt
      301 tcaggcttcc atcgcggaag cgcaccaggc tgtgaacgat gctctgcgga tgcaccacga
      361 tgtcgatctg ctcgggcgtc aggctgaaaa gccacttcgc ctcgatcgcc tcgaggccct
      421 tgttcatcag gctggcgctg tcgatggtga ccttggcgcc catgttccag ttggggtgct
      481 tcagcgcttc ggccgcggta acgtgctcca gttctgcgcg cgagcggccc cggaacgggc
      541 caccactcgc cgtcaggatc acctgctcga tcgggttgtc ccactcaccc gccatgcact
      601 ggaagatggc gctgtgctca ctgtccaccg gatagatgtt cacgcccttc tcacgcgcgg
      661 ccttggtaac gagttcgccc gccaccacca gggtctcctt gttggccaag gcgatggtct
      721 tgcccgcccc gatggcggcc agtgtgggac gcaagcccgc gtagcccacc atggcggtca
      781 gcaccgtatg gataccctcc atggtcaccg cctgctcaat gctttcagca cccgcgaaca
      841 ccttgatatc caagggctgc aacgcatcgc gcacagcggg gtaccgcgct ttgtcggcga
      901 tcaccaccgc gttgggcttg aattcccgcg cctgggcaat gagcaggtcg gcattgttcc
      961 cggccaccag cagttccacc gtgaaatggt ccggttgctc gcgcaccacc tgcagggcct
     1021 gcgtaccgat ggaaccggtg gagccaagga tggcgagtga tctcatgttg ccggggtgat
     1081 caggtgcctg cgtgcgcatg tggccatgtg cgcgctgcga gggcgcgaag atcgggtatc
     1141 ggggccgtgg tccgaccaga acgcgcggtg gggaggcgag ggccgatccg ccccaatgcc
     1201 acatcaacgc caatgcacca tgcgcatcac catcctcggg ccctgaaccg gaagttggat
     1261 cgccatggcc ccacagcgcg gtggccgtgg ccagtggtcg tgggggaaaa gccgtcgctc
     1321 tggtggcgca tcacaaggat gttccgtgcg ttgatgctgt cagcgtttcg ccgccgctct
     1381 ccgcaatctg tcgttgatcg cacggcccag gccctcctcc gggaaccgtt ccgcgatgat
     1441 cagatcggcc gcactggcat ccagttcgcg cagcacaccg aacaggcgac gagcggcttc
     1501 ggaaatatcc ccgttcggtg caagtgtttc gcattgccag gcgtcgtgaa cgttcctgaa
     1561 ggcgatcacc ccacaacgct catcggcatg ttcgcgcagg agccggtcca catcgcccac
     1621 gagaaccggc ttgcgcggcg cgtagtggct ctcgatcatt cccggtgcaa caggcagcac
     1681 ctgcttcacg tgcgggcgga ccgtgccgac cacttcttcg atgcgctcga gtgcgaggcc
     1741 cccgggccgg tacagcacgg gcagaccgtc ctcccacccg atgatggtgc tctccacgcc
     1801 gaccgtacac ggccctccat ccaggatata cgggattttt tcgccgagct ggtccgctac
     1861 gtgctgtgcg gtagtggggc tcacgtagcc gaacggattc gcgcttggcg cggccagcgg
     1921 gagatccagc gcacgcagga gc
//
LOCUS       DAOQOC010000613         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40485_length_1938_cov_2.943707, whole genome shotgun sequence.
ACCESSION   DAOQOC010000613 DAOQOC010000000
VERSION     DAOQOC010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40485_length_1938_cov_2.943707"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1304
                     /locus_tag="PL106_09940"
     CDS             126..1304
                     /locus_tag="PL106_09940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNA33429.1"
                     /translation="MSREVVIVSAVRTPIGSFGGGLAEVSSTELGATAVKAAVERAGV
                     SPDDVEEVIMGSVLQGNLGQAPARQASKFAGLPDKVRATTVNKVCASGMKAVMMATQD
                     IRLGDADVVVAGGMENMSRTPFYLEQARYGYRFGNGQVIDGISKDGLTDVYHQTAMGV
                     SADSTAKEHGITREEQDAFAMESYTRAANAWKAGKFKDEVVPVTVKSRKGDTVFAEDE
                     QFKAAKFEKIPVLKPVFTKDGTVTAANASAINDGAAALVLLSAEKAKALGLKPIARVL
                     GYADSEQAPDRFTTAPALAVPRAVEKAGLKMSDISYFELNEAFSVVGIANTRLMKLDP
                     AKVNANGGAVALGHPLGCSGARILVTLINVLKQNGAKYGAAGICNGGGGASAMVIEAI
                     "
     gene            1413..>1938
                     /locus_tag="PL106_09945"
     CDS             1413..>1938
                     /locus_tag="PL106_09945"
                     /inference="COORDINATES: protein motif:HMM:NF036854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNA33430.1"
                     /translation="MPSVICPLSIVPVRKEPSDRSEMVTQWLFGETAEVLERDGAWSR
                     LCFDHDGYEGWVDNKQITRCLTPNVEPDRRVIDQTSLVDLGDRQVLLPYGAVLPFLQD
                     GTILWDGRRVPVNAVTNVLGRNDRAGLIETYIHPFLGAPYLWGGRTPAGVDCSGFTQA
                     IFMLMGIYLRRDAYQ"
BASE COUNT          378 a          612 c          633 g          315 t
ORIGIN      
        1 agccactcac ccacctctcc gggtgtccgt gcagaaagaa caggttcccc tttcggggcc
       61 gcaaatatag aagcgcacat cgttcccgtg agcggctaaa ttcgcggccc caaacacaac
      121 agcccatgtc ccgcgaagtc gtcattgttt ccgcagtacg cacccccatc ggcagcttcg
      181 gcggtgggct ggccgaagtg agttccaccg aattgggtgc caccgctgtg aaggctgcgg
      241 tagaacgggc cggtgtatcg cccgatgacg tggaggaggt gatcatgggc agcgtgctcc
      301 agggcaacct gggccaggcc cccgcacggc aggcgtccaa attcgccgga ctgcccgaca
      361 aggtgcgggc caccacggtg aacaaggtgt gcgccagcgg catgaaggcc gtgatgatgg
      421 ccacgcagga catccgcctc ggcgatgccg acgtggtggt ggccgggggc atggagaaca
      481 tgagccgcac gcccttctat ctggagcagg cacgctatgg ctaccgcttc ggcaacgggc
      541 aggtgatcga cggtatcagc aaggacggcc tcacggacgt gtatcaccag acggccatgg
      601 gtgtgagtgc ggacagcacc gccaaggagc atggcatcac ccgcgaggag caggatgcgt
      661 tcgccatgga gagctacacc cgtgcggcca acgcgtggaa agcgggcaaa ttcaaggatg
      721 aagtggtgcc cgttacggtg aagagccgca agggcgacac ggtgttcgcc gaggacgagc
      781 agttcaaggc ggccaagttc gaaaagatac ctgtgcttaa accggtcttc accaaggacg
      841 gcacggttac ggcggccaac gccagcgcca tcaacgacgg cgcggccgcc ctggtgctcc
      901 tgagtgcgga gaaggcgaag gccctcggcc tcaaaccgat cgcgcgggtg ctgggctacg
      961 ccgattccga gcaggccccc gaccgcttca ccacggcgcc cgccctggcc gtaccccgcg
     1021 cggtggagaa ggccgggctg aagatgagcg acatcagcta cttcgaactg aacgaggcct
     1081 tctcggtggt gggcatcgcc aatacccgct tgatgaagct ggatcccgcc aaggtgaatg
     1141 cgaacggcgg ggctgtggcc ctgggccatc ccttgggatg cagcggcgcc cgcatcctcg
     1201 tcaccctgat caacgtgctc aagcagaacg gtgcgaaata cggtgctgcg ggtatctgca
     1261 acggcggtgg tggtgccagc gccatggtga tcgaagcgat ctgagatcgt tccatcggtg
     1321 tttgtagcac gaaacggccg gtggatcggg aacacgcgca acgcgaaccc gtaacccgaa
     1381 actcgtaacc cgaaacgccc tccctgcccc ccatgccctc cgtcatctgc cccctctcca
     1441 tcgtgccggt gcgcaaggaa ccgagcgatc gcagcgagat ggtgacgcaa tggctcttcg
     1501 gcgaaacggc cgaggtgctc gaacgggatg gggcgtggag ccggttgtgt ttcgaccacg
     1561 atgggtacga gggctgggtg gacaacaagc agatcacgcg ctgcctcacc ccgaacgttg
     1621 aacccgaccg tcgggtgatc gatcagacct ctctggtgga tctgggcgac cgacaggtgc
     1681 tcctccccta cggcgccgta ctgcccttcc tgcaggacgg taccattctg tgggacggcc
     1741 gccgtgtacc ggtgaacgcc gttaccaacg tgctgggccg gaacgaccgg gccggtctga
     1801 tcgaaacgta catccacccc ttcctgggtg cgccctatct gtggggtggt cgcacgcccg
     1861 ccggggtcga ttgcagcggg ttcacccagg ccatcttcat gctcatgggc atctacctgc
     1921 ggcgcgatgc ctaccagc
//
LOCUS       DAOQOC010000614         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40618_length_1935_cov_2.594149, whole genome shotgun sequence.
ACCESSION   DAOQOC010000614 DAOQOC010000000
VERSION     DAOQOC010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40618_length_1935_cov_2.594149"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            567..>1935
                     /locus_tag="PL106_09950"
     CDS             567..>1935
                     /locus_tag="PL106_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33431.1"
                     /translation="MNCHNTTSPLRTRWWGRMALMMAMALFGLSATQAQFVIVGTDGS
                     NTNGSGNDPIDDYYNFMRYQVIYTAAELTGAGLPSGATITGLGWSVSQDNGPAFPTYT
                     VRMAHTANANNGSHDGTALTTVFGPASYNPAVTTPGNHSMLTFGTNFVWNGTSNILVD
                     VCTGSSSMGFASPYGGVRGSTGTGGSRYVRCDGCGSQCSVNTNTTNTIKPQIRFNYLG
                     ATPCAGTPAPGSVPATLTACPNGSVVINATGTSTDTGISFQWEEWDGSTWVNAVGGSG
                     ATTTSYTTPALVTAMQYRLRTTCANGGGTDVTNTCNVSISMTPLCYCGGSLPTSNTDD
                     NTGLTRVVFNTIDQSSGGGPAYTDYTAVNTSVDAGANYNLAVQVNTAGNYTVYTKAWF
                     DWDRNGVFTDPGEEYDLGSAVNVANGAPNASPQMVSVPMITSGPVIMRARATYFTAPV
                     PCGNQN"
BASE COUNT          422 a          623 c          535 g          355 t
ORIGIN      
        1 gagcactgtt cttgtacact gcctgtgaac tgtcacgctg agcttgccga agcgccagca
       61 cgtagccgcg tcgggcgcct gcatcgtctt cgcgtagccg cttcggcgca gcaagaccag
      121 agcgctccgg cgtcgacgcg cgaagcgagg agcgcgatcc tttcttgaac ccgccggttg
      181 tgcccttgcg atgcactgcg cgggcacaac gcgcaggcgg ggccgcataa cccacgccgc
      241 tggccaacca tgtgtcctca gccatggatg ctgcgcacgg gatcaaccca ttctgaacgg
      301 catcctcaac tcagggggtg tgctgaagcg acctaagacc cctgttgata aattcatcga
      361 caactattga tttctgatag gcatttcaat aggtttggtc gaagagttcg cagtggtgct
      421 ccttccttga agaccaccgc gacctgaaca tcaaacccct cccccacaaa cgcttggcaa
      481 ccatcaacca cctgtatcac ccaccattga ccaacagccc gttggtcaat gaccatcacc
      541 gacaaccaac caaaacaacc cccaacatga actgtcacaa cacgacttcc cccctgcgca
      601 cgaggtggtg gggacgcatg gccctgatga tggcgatggc cttgttcggc ttatccgcta
      661 cgcaagccca gttcgtcatc gttggcaccg atggctccaa caccaacggc tccgggaacg
      721 acccgataga tgattactac aacttcatgc ggtaccaggt gatctacacc gctgctgaac
      781 tgaccggtgc cgggttgcct tctggagcta ccattaccgg tctggggtgg tccgtgtcgc
      841 aggacaacgg tcccgcgttc cccacctata cggtgcgtat ggcgcatacg gccaatgcga
      901 acaacgggtc gcatgatggc acggcactca cgacggtatt cggcccggcc agctacaatc
      961 cggctgttac cacaccaggc aaccacagta tgttgacatt cggcaccaac ttcgtttgga
     1021 acgggacgag caacattctg gtggatgtat gcacgggcag cagttcgatg gggttcgcaa
     1081 gtccctatgg aggggttcgc gggtcaacag gcacaggggg cagtcgctat gtgcgatgcg
     1141 atggatgtgg ctcacagtgc tcagtgaaca caaacaccac caacacgatc aaaccacaga
     1201 tccgtttcaa ttacctgggc gccaccccgt gtgcgggcac cccggcacct ggcagcgtac
     1261 cagcgacttt gacggcctgc cccaacggat ccgtagtgat caacgctacg ggcaccagta
     1321 ccgatacggg gatcagcttc cagtgggagg aatgggacgg cagcacctgg gtgaacgccg
     1381 tgggcggcag cggagccacc accaccagct acaccactcc cgcgctggtc accgctatgc
     1441 agtaccgcct gcgtaccacc tgcgccaacg gcggtggtac cgatgtgacc aacacctgta
     1501 acgtgagcat cagcatgacc ccgctgtgtt attgcggtgg atcattgccc acttcgaaca
     1561 ctgatgataa caccggcctt acccgcgtgg tgttcaacac catcgaccag agttctgggg
     1621 gcggccctgc ctacacggac tacaccgccg tgaacacttc ggtggacgcc ggagcgaact
     1681 acaacctggc cgtgcaggtg aacaccgcag gcaactacac ggtgtacacc aaggcttggt
     1741 tcgactggga ccggaacggc gtgttcaccg atccgggcga ggagtacgac ttgggcagcg
     1801 cagtgaacgt ggcgaatggc gcacccaacg cttcacctca gatggtctcg gtcccgatga
     1861 tcaccagcgg accggtgatc atgcgtgcac gcgccaccta cttcacagcc cctgtcccct
     1921 gtggcaacca gaact
//
LOCUS       DAOQOC010000615         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40718_length_1932_cov_3.941929, whole genome shotgun sequence.
ACCESSION   DAOQOC010000615 DAOQOC010000000
VERSION     DAOQOC010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40718_length_1932_cov_3.941929"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1027
                     /locus_tag="PL106_09955"
     CDS             227..1027
                     /locus_tag="PL106_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33432.1"
                     /translation="MKNRRIFLLLACSTILFVACRKDTNDNDLDLDYMSASDNARAED
                     AFTDLFNQVEKSMDENGLREACDPVVTFDTIAEPHSMTLDFGTVDCTAQNGRQRRGRI
                     HVTWTGRYRDPGTVITMTPDEYFVNGNQVQGTKTVTNLGPNDEGNLQFHVVVDGSLTA
                     GDGSWTATHQAQRTRTWIQGADTPGILDDVYLITGSGSGTNRNGVNYTVNITSPLRVE
                     LSCPFITAGSVDITPENRPTRTINYGNGSCDGTITITVNGHTFTVTIG"
     gene            1202..>1932
                     /locus_tag="PL106_09960"
     CDS             1202..>1932
                     /locus_tag="PL106_09960"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA33433.1"
                     /translation="MAYFSRVPVRYRTVLITAVVLATLFLAQAYMHHYVYKDLKDMGE
                     FSWRREAPVPYLNFLFWALLCPLVHIIHTRWPFTGRPLWRILLIHLGLGLCIAAFHEL
                     VTSGIYYALLQYIGDFDIRDPKYYTWALHALVPAIFTRTMEYVVLMGLLVALEDARLR
                     RVDREQLLRLRNELHVSQLNALKKQLRPHFLFNTLNTVSALMDEHPADARKVLSRLGR
                     LLRNTLDKTERDTVPLEHELDHIRD"
BASE COUNT          382 a          642 c          556 g          352 t
ORIGIN      
        1 ggggcgatgt gaagttcccg gagatcggca agggctggaa ggaggtgtgg accgaacggc
       61 acgagaagga cgagcggcac gcgcacgcct tcaccttcaa ggtgctccag cgctgagccg
      121 gggccgggta acgcatcggt aaagggatca ttgtgacgga tcctccgtgt acccgatcaa
      181 accctttcgc cgttgaacgg tcagaaaggc actaacgcac acttccatga agaaccggag
      241 aatattcctg ctgctcgcct gtagcaccat tctgttcgtc gcgtgccgca aggacacgaa
      301 cgacaatgac ctcgacctgg actatatgag cgcgagcgac aacgcccgcg cggaagacgc
      361 cttcaccgac ctgttcaatc aggtggagaa gtcgatggat gagaacggct tgcgcgaggc
      421 ctgcgacccg gtggtcacct tcgacaccat cgccgagccc catagcatga ccctcgattt
      481 cggcacggtg gattgcaccg cacagaacgg ccgccagcgc cggggccgca tccatgtgac
      541 ctggaccgga cgctaccgag atccgggtac ggtgatcacc atgaccccgg acgaatactt
      601 cgtgaatggc aaccaggtgc agggcaccaa gaccgtgacg aacctcggtc ccaacgacga
      661 gggcaacctc cagttccacg tggtggtgga cggtagcctc actgctgggg acgggagttg
      721 gaccgccacg caccaagctc agcgcacccg cacctggatc cagggagcgg acacgcccgg
      781 catcctggat gatgtgtacc tgatcaccgg cagcggcagc ggaacgaacc gcaacggcgt
      841 caactacacc gtgaacatca caagcccgct gcgcgtcgag ctgagttgtc ccttcatcac
      901 agcggggtcg gtggacatca ccccggagaa caggcccacc cgcaccatca actacggtaa
      961 tgggtcctgc gacggtacca tcacgatcac cgtgaacggc cacaccttca cggtgacgat
     1021 cggctgaccg gaggctgtac aggcatgcga gggtgcgggg catacttccc gcaccctcgc
     1081 tttttccgga ccaccccgtc cccatcccgg gcgctaaccg ctcgtcccgg tcctggtccc
     1141 ttgtcactgt tcgtcccacg ggcgtcggac cccgggctag cttcgttgct cttcggtgcc
     1201 catggcctac ttcagccgcg tcccggtccg ctaccgcacg gtgctcatca cagcggtggt
     1261 gctcgccacg ctcttcctcg cgcaggccta catgcaccac tatgtgtaca aggacttgaa
     1321 ggacatgggc gagttcagtt ggcggcgtga ggccccggtg ccctatctca atttcctgtt
     1381 ctgggccctg ctatgcccgc tggtccacat catccacacc cgctggccct tcaccggacg
     1441 tccgctttgg cgcatcctgc tcatccacct gggcctgggt ctctgcatcg ccgccttcca
     1501 cgagctggtc acctccggga tctactacgc cctcctgcag tacatcggcg atttcgacat
     1561 acgcgacccg aagtattata catgggcttt gcatgccctg gtgccggcga tcttcacgcg
     1621 caccatggag tacgtggtgc tgatgggcct cctggtggcc ctggaggatg cccgcttgcg
     1681 ccgggttgat cgtgaacaac tcctccgctt gcgcaacgaa ttgcacgttt cgcagctcaa
     1741 cgcgttgaag aaacagctca gaccgcactt cctgttcaat accttgaaca cggtaagcgc
     1801 cctgatggac gagcacccgg ccgatgcccg taaagtgctc agccgcctgg gacgcctgtt
     1861 gcgcaacacc ctggacaaga ctgagcgcga tacggtaccg cttgaacacg aactggacca
     1921 tatccgcgat ta
//
LOCUS       DAOQOC010000616         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40727_length_1932_cov_3.239744, whole genome shotgun sequence.
ACCESSION   DAOQOC010000616 DAOQOC010000000
VERSION     DAOQOC010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40727_length_1932_cov_3.239744"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PL106_09965"
     CDS             <1..1067
                     /locus_tag="PL106_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33434.1"
                     /translation="ATTPRSWPRRPWSAVLVSLLVCAATLCIVHSTLPPFWGVPNLAI
                     KWDALHRDPIPQRALIGSSRIYRQAIPSIIDSVIGQGRTFNAGYAATFPPATYEFLEQ
                     LLREERQLPEEILVELSNYMLFEEANLEDHRYWYYLGPREGWGLVRHALFLPNQPRAE
                     RLGFAARAARATTCSLLGLGLADHLLAATADTDTLLALGPAGDGGLTLETLLRYRPDD
                     EGQLARRATFRADTLAALRRAVGLRHLYERPSTGGVSLVHLSRLTALLDKAHSRGVRL
                     RFVLPPLWIDRGEDLLALVHALPDGAVIDLCHPDSFPQFYRPENMFDAGHLNDRGARL
                     FSMELARALHAPGHNPEFAR"
     gene            1071..>1932
                     /locus_tag="PL106_09970"
     CDS             1071..>1932
                     /locus_tag="PL106_09970"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA33435.1"
                     /translation="MLFNSAEFCLLFFPLVTGLYFLLPKNWRWLLLLLASCWFYMAFV
                     PLFILILAFTIGVDFVAGIAIARSEGPRRKAYLVASIIANVGVLAVFKYWDFLNGNLT
                     ALFAGMGLEYHVPKLHEWTFFGFLPAGMVLPIGLSFHTFQSLSYTIEVYRKHQQAERR
                     FGTFALYVMFYPQLVAGPIERPGNLLNQLNATDGHGIRSDFDHHRVVQGLKQMLWGFF
                     KKLVIADRCAALVDIGYTHPAAQDGGSLLLATLFFAFQIYCDFSGYTDIALGAARVMG
                     FNLMVNFCTPY"
BASE COUNT          311 a          654 c          564 g          403 t
ORIGIN      
        1 atgccaccac gcccaggagt tggccgagac gcccgtggag cgctgttctc gtctctctcc
       61 tggtgtgtgc ggccacgctg tgcatcgttc acagtaccct tccgcctttc tggggtgtgc
      121 ccaacctggc catcaaatgg gatgcgctgc accgggatcc gatccctcaa cgggcgctca
      181 tcggttcgag ccgcatctat cgacaggcga tcccttccat catcgatagc gtgatcggac
      241 agggccggac gttcaatgcc ggttacgccg ccactttccc tccggccacg tacgaattct
      301 tggaacaact gctccgtgaa gaacgccagc ttcctgagga gatcctggtc gaactctcca
      361 actacatgct cttcgaggag gcgaaccttg aagatcatcg ctattggtac tatctgggac
      421 cacgggaagg gtggggcctg gtgcgccacg ccttgttcct cccgaaccag cctcgtgcgg
      481 aacgactggg tttcgccgcg cgcgccgcgc gtgccaccac gtgctccttg ctgggcctcg
      541 gtctggccga tcacctgctg gcggccactg ccgacaccga tacgctgctc gcgctgggac
      601 ccgctggcga tggggggctc acactggaga ccttgctgcg gtacaggccc gatgatgagg
      661 gccaactggc ccgccgcgca acattccggg cggacacgct cgcagcgctg cgccgtgcgg
      721 tcggtctgcg ccacctctac gaacgccctt cgaccggagg tgtgagcctc gtccatctga
      781 gccgcttgac cgcattgctc gacaaggctc acagtcgggg cgtccgattg cgattcgtgc
      841 tgcctccgct ctggatagac cggggagagg acctgcttgc cctcgtgcat gcgctgccgg
      901 acggtgccgt catcgatctg tgccatccgg actcgttccc tcaattctac cgccccgaga
      961 acatgttcga cgctgggcat ctcaacgacc gcggagcaag gctcttcagc atggaactgg
     1021 cccgcgccct gcacgcaccc ggccacaacc cggaattcgc gcgatagacc atgctcttca
     1081 acagcgccga gttctgcctg ctcttcttcc ccctggtcac cgggctgtac ttcctgctgc
     1141 cgaagaactg gcgctggctc ctgttgctgc tggccagctg ctggttctat atggccttcg
     1201 tcccgctctt catcctgatc ctcgccttca ccatcggggt ggacttcgtg gccggcatcg
     1261 ccatagcgcg cagcgaggga ccgcggcgaa aggcatacct cgttgccagt atcatcgcca
     1321 atgtgggggt gctggcggtg ttcaagtact gggacttcct caacggcaac ctcacggccc
     1381 tgttcgccgg gatgggactg gaataccatg tgcccaagtt gcacgagtgg accttcttcg
     1441 gctttctccc ggccggcatg gtgctgccca tcggtctgtc gttccacacc tttcagagct
     1501 tgagctacac catcgaggtc taccgcaagc accaacaggc cgagcgccgc ttcggcacct
     1561 tcgcgttgta cgtgatgttc tacccgcaac tggtggccgg ccccatcgag cggccgggca
     1621 atctgctcaa ccagttgaac gccacggatg ggcacggtat ccggtccgac ttcgaccacc
     1681 accgggtggt ccagggcctc aagcagatgc tctggggctt cttcaagaaa ctcgtgatcg
     1741 cggatcgctg tgccgcgctc gtggacatcg gatacaccca tccggcggca caggatgggg
     1801 gttcgctgct gctggccacg ctcttcttcg cgttccagat ctattgcgac ttcagcggct
     1861 ataccgacat cgcgcttggc gccgcccggg tgatgggctt caacctgatg gtgaacttct
     1921 gcactccgta cc
//
LOCUS       DAOQOC010000617         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40792_length_1930_cov_3.899200, whole genome shotgun sequence.
ACCESSION   DAOQOC010000617 DAOQOC010000000
VERSION     DAOQOC010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40792_length_1930_cov_3.899200"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PL106_09975"
     CDS             <1..699
                     /locus_tag="PL106_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33436.1"
                     /translation="PLMLGGDDLMLVCQAELALPFMCTLCKALDGLQANAGNGFKLTL
                     GIGVIIAKHTVPIHHLHNVVGQLVDSAKQRYRSIPEGQTKYSVIDWGVHSTSWIGDLG
                     DDRQHDWVRGTGKELRLLSQRPVIVNGDEFDSLEGLLKGAEKLKGAPRSQLRFLVEQL
                     PRGRALAELAFAELSAGARKALNDLGITEPWQAGQSDAPRHTPLLDLVELAEIQRLGR
                     TVRAEPTTTQETLA"
     gene            729..>1930
                     /locus_tag="PL106_09980"
     CDS             729..>1930
                     /locus_tag="PL106_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33437.1"
                     /translation="MSTPTAAAPAPGQWVRYKLDAELLSDMHPGSGLGAGGIDAVVAR
                     DRRGRPVIHASHIEGVLRDAARRLHGNEAAGRFFGLAGGQHQRAAFTSLYTDEAIGTH
                     VWRSSAREDFDNRAPKDDTLHTVEYVPKGTRFSGMVELPSSDVALLERLVQEVDALGA
                     GRATGAGRMKLTLTGTTPAPRPMGEATGRLRILLRGLDPLCITSSATPDNLIPSQAYI
                     PGRTLLGALAAWLIKEGATHEASMLTTGDVLVSDALPTSDRPDDLTQVEVLPAPLSLQ
                     SEKPMGRGSKLPWWAAQKGPTQREDALASDGKLKRPESDLFLCRREAAKEWTAYRPVR
                     GIQLRNGRPEPGQPEPSLFAIEQIAEGTHFVAELSGPVKAMRELTRQLAPIIQGTRWL
                     RVGRGGAPV"
BASE COUNT          399 a          611 c          586 g          334 t
ORIGIN      
        1 cccctgatgc tcggcggcga tgacctgatg ctcgtgtgcc aagccgaact cgcgttgccc
       61 ttcatgtgta ccctttgcaa ggccttggac ggtcttcagg ccaacgcggg caacgggttc
      121 aagctgaccc ttggcatcgg tgtgatcatt gccaagcata cggtgcccat acaccacctg
      181 cacaatgtgg tggggcagtt ggtcgactcg gccaagcagc gctatcgcag cattcccgaa
      241 ggccaaacca aatattccgt gatcgactgg ggcgtacaca gcacctcgtg gatcggtgac
      301 ctgggtgacg accgccaaca cgattgggtg aggggcacgg gtaaggagct tcgccttctt
      361 tcccaacggc cggtaattgt gaacggagat gagttcgatt cccttgaagg actgctcaag
      421 ggtgccgaaa aactcaaggg cgcaccgcgc tcacagcttc gctttctggt tgagcaacta
      481 ccacgtgggc gggcgcttgc cgaactggca ttcgccgaat tatccgcagg tgcaaggaaa
      541 gcgctgaacg acctcggtat taccgaacca tggcaagcgg gccagagtga tgccccgaga
      601 cacacgcccc tattggacct tgtggaactg gccgagatcc aacggctggg gcgaacagta
      661 agggccgaac caaccaccac gcaagaaacc cttgcttaag ccataacaga atggacgaac
      721 aagcaagtat gagcacccca acggccgctg cgcccgcccc cgggcagtgg gtgcgctaca
      781 agctcgatgc ggagctcctg tcggacatgc acccgggatc gggcttgggc gcaggcggca
      841 ttgatgccgt ggtggcccgc gaccggcgtg ggcgtcccgt gatccatgcc tcgcacatcg
      901 agggggtgct tcgcgatgcg gcccgcaggc tgcatggaaa cgaggccgca ggccggttct
      961 tcggactcgc gggcggccaa caccaacgcg ctgctttcac ttcgttgtac accgacgaag
     1021 ccatcggcac ccatgtgtgg cgttcctccg cacgggaaga cttcgacaac cgggccccca
     1081 aagatgatac cctgcacacg gtagagtacg tacccaaagg cacccggttc tccggtatgg
     1141 tggaactacc ttccagcgat gtggcgttgc tggaacggct ggtgcaggaa gtggacgcct
     1201 tgggtgctgg ccgtgccacg ggcgcgggcc ggatgaaact taccctgact ggcaccactc
     1261 ctgcaccccg gcccatgggc gaggcaaccg gccggttgag gatcctgctc cgtggcctgg
     1321 accctctttg catcacctct agtgccacac cggacaacct gatccccagc caagcataca
     1381 tccccggccg caccctcctc ggagcgttgg ctgcttggct catcaaggaa ggggccacgc
     1441 atgaggcatc catgcttact acaggtgatg ttctcgtcag cgacgccttg cccacctccg
     1501 accggccgga tgacctgacc caagttgaag tacttcccgc cccgctatcc ctgcaaagcg
     1561 aaaagcccat gggaagaggc agcaagctgc cgtggtgggc ggctcaaaaa gggcctaccc
     1621 aaagagagga tgcattggcc tccgatggaa agctcaagcg tccggaaagc gacctcttcc
     1681 tgtgccgaag ggaagcagca aaggagtgga ccgcctaccg ccccgtacgc ggtatccaat
     1741 tgcgcaacgg gcggccggag ccgggacaac cggaaccctc gctcttcgcc attgaacaaa
     1801 tagcggaggg cacccacttt gtcgcggaac tttccggccc cgtgaaagcc atgcgggaac
     1861 tcacccggca actggctccg atcattcaag gtacccgatg gctgcgtgtg ggccggggtg
     1921 gtgcgcccgt
//
LOCUS       DAOQOC010000618         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40798_length_1930_cov_3.606933, whole genome shotgun sequence.
ACCESSION   DAOQOC010000618 DAOQOC010000000
VERSION     DAOQOC010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40798_length_1930_cov_3.606933"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1528)
                     /gene="radA"
                     /locus_tag="PL106_09985"
     CDS             complement(137..1528)
                     /gene="radA"
                     /locus_tag="PL106_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNA33438.1"
                     /translation="MAKVRSLFVCQNCGASFPQWLGQCGSCKEWNTLVEEVQDRVQEK
                     RSLPDSVRERKPKPVAIAEIAADSGPRMPLGDRELDRVLGGGVVPGSLILMGGDPGIG
                     KSTLMLQTVLRMDGVRTLYVSGEESEQQVKMRADRLEATGHRPPAADGAPTGCYVLTE
                     TNTQNLFKQLEALRPGLVIVDSIQTLHTAALDASPGSVGQVRECTSELMRFAKTTGVP
                     IVLIGHITKDGSIAGPKVLEHMVDCVLQFEGDRDHAFRLLRPLKNRFGSTNELGIYEM
                     RGSGLAVVEDPSRVLLGDRGERPSGVCVAATLEGMRPLLIEVQALVSSAVYGTPQRSA
                     TGFDLRRLNMLLAVLEKRCGFRLAQKDVFLNLAGGFRVEEPAIDLAVVCAVLGSNADI
                     PIPIDTAFCGEVGLTGEIRPVPRTEQRIAEAAKLGFQRIFIPQGTKGLPKDPGIELVA
                     VARVDQVLSHLFG"
     gene            complement(1793..1924)
                     /locus_tag="PL106_09990"
     CDS             complement(1793..1924)
                     /locus_tag="PL106_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33439.1"
                     /translation="MGTTKSYISKIENNLKEVRISTLMRIVELGFGGQLQLSIKLQR"
BASE COUNT          335 a          620 c          622 g          353 t
ORIGIN      
        1 agcgtggtga gcaagtggga gggggagctg ggtcattgac aagtccgcct ccgccgaggc
       61 tgtttcggag gaattggaac acagcgcccg cggaccgccg caacgctcgg agaccttgtc
      121 cggtcaggat acaagcctat ccgaaaagat gtgacagtac ttggtccacg cgggccacag
      181 cgaccaattc gatgccggga tccttgggca agcccttggt gccctgcgga atgaaaatgc
      241 gttggaaccc caatttcgct gcttccgcga tgcgctgttc ggtccgtggc acggggcgta
      301 tctcgccggt gagtcctact tctccgcaga atgcggtatc gatggggatg gggatgtcgg
      361 cgttggagcc gagcacggcg cacaccacgg ccaggtctat ggcgggctct tccacgcgga
      421 aaccacccgc cagattgagg aacacgtcct tctgtgcgag gcggaagccg cagcgtttct
      481 ccagcacggc cagcaacatg ttcaggcggc gcaggtcgaa accggtggca ctgcgctggg
      541 gcgtgccgta caccgccgag ctcaccaggg cctgcacctc gatcagcagt ggccgcatgc
      601 cttccagcgt ggctgctacg cacacaccgc tgggccgctc accgcggtcg cccaacagca
      661 cacggctggg gtcctccacc actgccagcc cgctgccgcg catttcgtag atgcccagtt
      721 cattggtgct gccgaagcgg ttcttcaggg ggcgcagcaa tcggaaggca tggtcgcggt
      781 cgccctcgaa ctgcaacacg caatccacca tgtgctccaa caccttcggc ccggcgatgc
      841 tgccatcctt ggtgatgtgc ccgatgagca cgatgggcac gccggtggtc ttggcgaagc
      901 gcatgagctc gctggtgcat tcgcgcacct ggcccacgct gccggggctg gcgtccagcg
      961 cggcggtgtg cagcgtctgg atactgtcca cgatcaccag tccgggccgc aacgcctcca
     1021 gttgcttgaa gaggttctgg gtgttggtct cggtcaatac gtagcaaccg gttggggctc
     1081 catcggcggc cggtggccgg tggccggtgg cctccaaccg gtcggcgcgc atcttcactt
     1141 gttgttcgct ctcctccccc gagacgtaca gggtgcgtac accgtccatg cgcagaacgg
     1201 tctgcagcat caacgtgctt ttaccgatac ccggatcccc gcccataagg atcaatgatc
     1261 ccggcaccac cccgccgccc agcacgcggt ccagctcgcg gtcgcccaag ggcatgcgtg
     1321 gcccgctgtc cgcggcgatc tcggcgatgg ccaccggctt cggcttgcgc tcgcgcacgc
     1381 tgtcgggcag ggagcgcttc tcctgcacac ggtcctgcac ctcctccacc aaggtgttcc
     1441 attccttgca gctgccgcat tgccccagcc actgcgggaa ggaagcgccg cagttctggc
     1501 agacgaagag ggaacggacc ttggccatgt gtggggagcg cgtgttcagt ggaagggcca
     1561 aaggtgagca aggaaggccg gaagacgcgc tgaacccagg cagtggccac aggcgcggca
     1621 ccaaccgggt agtggcacta ccacttcgct ctttcaggca ggggtgtggt gccgtccagc
     1681 accgagggag cgcaccgctt tcgggtggcc gggcaacagg aaagagcgac cgggtggtcg
     1741 cgcccgatac gggctctctt cttgtacgcg aacacgcgca cgctggcccc ggtcaacgct
     1801 gtagtttgat ggacagttgg agttgcccgc caaaaccgag ttccacgatc cgcatgagcg
     1861 tggaaatgcg gacctctttg aggttgttct cgatcttcga gatgtaggat ttcgtcgttc
     1921 ccaccttctc
//
LOCUS       DAOQOC010000619         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_40995_length_1925_cov_3.102674, whole genome shotgun sequence.
ACCESSION   DAOQOC010000619 DAOQOC010000000
VERSION     DAOQOC010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40995_length_1925_cov_3.102674"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PL106_09995"
     CDS             complement(<1..705)
                     /locus_tag="PL106_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNA33440.1"
                     /translation="MEIPAPATMTPAMEPTGSRPPIHVTRTFLPPQEEYLHWLRKAYA
                     SHHLTNQGPIHQELEETLRVRSDVPYLRLMANGTLALQLAIRALGVQGKVITTPFSYV
                     ATTSAILWEGCEPVFADIDPGTCCIDPAAIEAAITPGVTAILATHVYGIPCDVEAIDA
                     IAKRHGLKVIYDAAHAFDVTYKGQSILQWGDASTLSFHATKLFHTVEGGAVVLHDAAA
                     DERLRLLRSFGHRNDDH"
     gene            complement(721..>1925)
                     /locus_tag="PL106_10000"
     CDS             complement(721..>1925)
                     /locus_tag="PL106_10000"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNA33441.1"
                     /translation="MRSFPGAVRKRLAGLRDRVKGRIDRLREERIVQRCEADLRTMPA
                     RTLAVLLPFPGHLKFVADTLAEVRAKGVTLHCFTDEAFILELRRSLGTDHVYPYRLCP
                     RLRYRVILTPATHIPFKKYKNPGSVVVHLPHSMVSLHTIFPADTFLGFDHVLCCGPHH
                     VREITAIFKHSGQRGQAWETGYEVIDRLGREVGGRSGHRRPCILIAPTWGPSSLLYRF
                     GRDLVDRLTPHYDVILRPHKWKMEEIAGMLDDLRRDHTGNQGFAIDLDADPKPSMEAA
                     DLMISDHSGAAFEFALGCLRPVVFIDGPRKNAHINWREVLDEEGIEVIGRTRIGTVVK
                     DLDEAENTIRSLLADPGSAEQKGRKAREEILFNYGRCAPVAAERLLEILGTPPANATH
                     GGGTARYI"
BASE COUNT          320 a          604 c          639 g          362 t
ORIGIN      
        1 gtggtcgtcg ttccggtggc cgaaactccg caacaggcgc aggcgctcat cggcggccgc
       61 atcgtgcagc accaccgcgc caccttccac cgtgtggaac aatttggtgg catggaagct
      121 cagggtgctg gcatcacccc attgcaggat gctctgcccc ttgtaggtca catcgaacgc
      181 atgggccgcg tcatagatga ccttcaggcc gtgtctcttc gctatggcat cgatcgcctc
      241 cacatcgcag ggtatgccgt acacatgcgt ggcgaggatg gcggtgacgc cgggtgtgat
      301 ggccgcctcg atggccgccg gatcgatgca acaggtgccc gggtcgatgt ccgcgaacac
      361 cggttcgcag ccctcccaga ggatggcact ggtggtggcc acgtagctga acggtgtggt
      421 gatcactttg ccctggacgc ccaaggcgcg aatggccagt tgcagcgcga gggtaccgtt
      481 ggccatcagg cgcaggtagg gtacatccga acggacgcgc aaggtctctt ccagttcctg
      541 gtggatgggg ccctgattgg tgaggtggtg cgaggcatag gcctttcgca gccaatgcag
      601 atactcctct tgcgggggta ggaaggtgcg cgttacatgg atcgggggcc gggatccggt
      661 gggctccatc gcgggcgtca tcgtggcggg ggcggggatc tccatcaggg ttgcgcttcg
      721 tcaaatatag cgggctgttc cgcctccatg ggtcgcgttg gcgggaggtg tccccaggat
      781 ctccagcagg cgttccgcgg ccacgggtgc acaccggccg tagttgaaca ggatctcttc
      841 acgggccttc cttcccttct gctccgcgct ccccggatcg gcgagcaggg accggatcgt
      901 gttttccgct tcgtccaggt ccttcaccac cgttccgatc cgcgtgcgac cgatcacttc
      961 gatgccctcc tcgtccagca cttcacgcca gttgatgtgt gcgttcttgc gcggaccgtc
     1021 gatgaacacc accggacgca ggcacccgag ggcgaactcg aaggccgcac ccgaatggtc
     1081 cgagatcatc aggtcggcgg cctccatcga gggtttgggg tccgcgtcca gatcgatggc
     1141 gaagccttgg ttcccagtgt ggtccctgcg cagatcgtcc agcatgcccg cgatctcctc
     1201 catcttccat ttgtggggcc gcaggatcac atcgtagtgc ggggtgagcc ggtccaccag
     1261 atcccgcccg aaacggtaca gcaggctgct gggtccccac gtgggggcga tcaggatgca
     1321 tgggcggcga tgtccggacc tgccgcccac ctcgcgcccc aagcggtcga tcacttcata
     1381 gcccgtttcc caggcctgtc ccctctggcc gctgtgcttg aagatggccg tgatctcccg
     1441 cacatgatgc ggcccgcaac agagcacgtg gtcgaaaccc aggaaggtgt ccgccgggaa
     1501 gatcgtgtgc aggctcacca tcgaatgcgg aagatgcacc acgacgctac ccgggttttt
     1561 gtacttcttg aacgggatgt gtgtggccgg ggtgaggatg acccggtagc gaaggcgggg
     1621 gcacaaccgg tacgggtaca catgatcggt gccgagcgag cgccgcagct cgagaatgaa
     1681 ggcctcgtcc gtgaaacagt gcagcgttac gcccttggcc cgcacttcgg ccaacgtgtc
     1741 ggccacgaac ttcaggtggc ccgggaaggg caggagcacg gccagggtgc gggcgggcat
     1801 cgtgcgcagg tccgcttcac agcgctgaac gatgcgttcc tcccgcaggc gatcgatgcg
     1861 acctttcacc cggtcgcgca ggccggccag gcgcttccgc accgcaccgg ggaatgacct
     1921 catgg
//
LOCUS       DAOQOC010000620         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41017_length_1924_cov_3.926699, whole genome shotgun sequence.
ACCESSION   DAOQOC010000620 DAOQOC010000000
VERSION     DAOQOC010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41017_length_1924_cov_3.926699"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PL106_10005"
     CDS             <1..1079
                     /locus_tag="PL106_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNA33442.1"
                     /translation="DRQPEFTQIDCEMAFVEQEDILRMFEGLAKHLFQSVLGKEFTAP
                     FPRMTFDEAMRNYGCDKPDLRFGMKFQELNDLAQGKGFKVFDQAELVVGINAEGCANW
                     SRKQTDALIDFVKRPQIGATGIVFLKWTEEGLKSTVDKFYSSEQLQQWAERCGMKQGD
                     LLCIMAGKADRTRKQLNELRLHLGDLCNLRDPWTFTPLWVVDFPLLEQDEESGRWHAM
                     HHPFTAPKPEDAQKLFDQKDLGSVRANAYDLVINGTEIGGGSIRIHERAMQEAMFRVL
                     GFSDEDARYKFGFLMDAFEFGAPPHGGAAFGFDRWVSLFGGRSDIREFIAFPKNNAGR
                     DTMIDAPSRIDAEQLKDLGLATKG"
     gene            complement(973..>1924)
                     /locus_tag="PL106_10010"
     CDS             complement(973..>1924)
                     /locus_tag="PL106_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33443.1"
                     /translation="LLALAGSMAMLMAQAQQDWNRLPGGDVINSTEYLGARLGSSVPL
                     VLKTVPALPIDFYTTDIFRARINPRVQYPLLNTFPNIPADGFTLITPDIGFLGQAPRG
                     PFSRLHLAEGGTTGNAEQFGYRPWQKNGITFTGNDDQGYLGQKYRDGESGYTDMVMQW
                     SDNPGAVRADRLRFLFTSAYTGATSGMNSREGLEGMRLWPTWENRINVGIGDFYAGNL
                     IDPVNIIDPTERLDVLDGRVRIRQLPNDPEANTMDRYIYGTSPSCRVPQTRICGTYLT
                     WEGGSITPSSPGPGPSTVPHRSGWAHRSWCRGRRYSWGRR"
BASE COUNT          378 a          583 c          593 g          370 t
ORIGIN      
        1 ccgaccgcca gccggagttc acccagatcg attgcgaaat ggccttcgtg gaacaggagg
       61 atatcctgcg gatgttcgag ggtctggcca agcacctctt ccaaagcgtg ctgggcaagg
      121 agttcaccgc accctttccc cgcatgacct ttgatgaggc catgcgcaac tacggctgcg
      181 ataaacccga cctgcgtttc gggatgaaat tccaggagtt gaacgacctc gctcaaggga
      241 aaggcttcaa ggtcttcgac caggccgaat tggtggtggg tatcaatgcc gaaggttgcg
      301 ccaactggag ccgcaagcag accgatgcgc tcatcgactt cgtgaagcgc ccgcagatcg
      361 gggccacggg catcgtgttc ctgaagtgga ccgaggaagg gctgaagagc accgtggaca
      421 aattctattc gtcggaacaa ctgcaacagt gggccgaacg ttgcggcatg aaacagggcg
      481 atctcttgtg catcatggcc ggcaaggccg acaggacgcg gaagcaactg aacgaactgc
      541 gcctgcacct gggcgacctg tgcaacctgc gcgatccctg gacattcacg ccgttgtggg
      601 tggtggactt ccccttgctg gagcaggacg aggagagcgg gcggtggcac gccatgcacc
      661 atcccttcac cgcgcccaag cccgaggatg cacagaagct cttcgaccag aaggacctcg
      721 gcagcgtgcg cgccaacgcc tacgacctgg tgatcaatgg gacggagatc ggcgggggca
      781 gcatccgcat ccacgaacgc gccatgcagg aggccatgtt ccgcgtgctg ggattctccg
      841 atgaagatgc ccggtacaaa ttcggtttcc tgatggatgc cttcgagttc ggcgcgcccc
      901 cgcacggcgg cgcggccttc ggcttcgacc gttgggtctc gctcttcggc ggccgcagcg
      961 acatccgcga gttcatcgcc ttccccaaga ataacgccgg ccgcgacacc atgatcgatg
     1021 cgcccagccg gatcgatgcg gaacagttga aggacctggg cctggcgacg aaggggtgat
     1081 ggatcctcct tcccaggtca ggtatgtgcc gcagatcctg gtttgtggga ccctgcagga
     1141 gggggatgtg ccgtaaatgt acctgtccat ggtattcgcc tccggatcat ttggcaactg
     1201 ccggatccgt accctcccgt ccagcacatc caaccgttcg gtggggtcga tgatgttgac
     1261 cggatcgatc aggttgcccg cgtaaaagtc gccgatgccc acgttgatcc ggttctccca
     1321 tgtgggccac aaccgcatgc cttccaggcc ctcgcgcgaa ttcataccgg aggttgcacc
     1381 cgtgtatgcg ctggtgaaca ggaaacgcag ccggtccgct cgtactgcac ccggattgtc
     1441 gctccattgc atcaccatgt cggtgtagcc cgactcccca tcccggtact tctgcccaag
     1501 gtatccttga tcatcattcc ccgtgaaggt gatgccgttc ttctgccacg gtcggtagcc
     1561 gaactgctcg gcattgcctg tggtgcctcc ctcggcaagg tgcaagcggc tgaacgggcc
     1621 ccggggtgct tggcccagga agccgatgtc cggggtgatc agcgtgaaac catccgccgg
     1681 gatgttcggg aaggtgttca acaaggggta ctgcaccctc gggttgatcc gcgcgcggaa
     1741 aatgtccgtg gtgtagaagt cgatgggcag ggccggtacg gtcttcagta ccaacggcac
     1801 ggaactaccc aaccgcgcac ccaggtactc ggtgctattg atcacatcac cgccgggcag
     1861 gcggttccaa tcttgttgtg cttgggccat aagcatggcc atgcttcctg ccaaggcaag
     1921 aagc
//
LOCUS       DAOQOC010000621         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41111_length_1922_cov_3.485270, whole genome shotgun sequence.
ACCESSION   DAOQOC010000621 DAOQOC010000000
VERSION     DAOQOC010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41111_length_1922_cov_3.485270"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PL106_10015"
     CDS             complement(<1..568)
                     /locus_tag="PL106_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA33444.1"
                     /translation="MKTQFAACLTVSVLLASCVPALKMREARKVDRTYYAGATDTTND
                     ANITWRTFFKDPDLHALIDSALGNNQELNIMLQEIEAARAEVRARKGEYLPFVDFGAA
                     AEVDKVGEFTRNGAVEHDLDVKEETKFPEPLPNYMIGARASWELDIWKKLRNAKKAAM
                     MRYLGTVEGKNFMVTHLVAEIANSYYELM"
     gene            complement(596..>1922)
                     /locus_tag="PL106_10020"
     CDS             complement(596..>1922)
                     /locus_tag="PL106_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA33445.1"
                     /translation="RHKDVNEVMEELEHLTKDLGAVIEYFEPPAVPGFGSSGGFSLRM
                     LDRTNGTDYHEFDRVNKDFMAALAKRKELTGLFTFYAANYPQYELIVDNQVAMQKGVS
                     IGKAMENLNILIGSDYQQGFIRFGRYFKVFVQAGPEYRRYPSDLADLFVKNEEGEMVP
                     YSSFMRFEKRLGPNEITRYNMYNSAAIRGLPAAGYTSGDAINAIKEVAKASLPRGYDI
                     AWEGLSFDEAQRGNEAVYIFLIVLIFVYLVLAAQYESFVLPLVVLLSLPVGVLGSFLM
                     LKMMGLANDVYAQIGIIMLIGLLGKNAVLIVEFAVQKQRAGATVREAAIEGARARFRP
                     ILMTSFAFIAGLIPLVVATGAGAIGNRTIGSSALGGMFVGTLLGVLIIPGLYFVFATM
                     IQGRKLISDERDEPVSEQFIRSAEEGSSAKETIRHLNDRLKELLKRKKD"
BASE COUNT          348 a          612 c          567 g          395 t
ORIGIN      
        1 ccatcagttc atagtagctg ttcgcgatct cggccaccag atgggtcacc atgaagttct
       61 tcccctccac cgtgcccaag tagcgcatca tggccgcttt cttggcattg cgcagcttct
      121 tccagatgtc caactcccac gaggcacgcg ccccgatcat gtagttgggc aatggttccg
      181 ggaacttggt ctcctccttc acatccaggt cgtgctccac ggcaccgttc cgcgtgaact
      241 cgcccacctt gtcgacctcg gccgccgcac caaaatccac gaagggcagg tactcccctt
      301 tgcgcgcacg aacctcggcc cgtgccgctt cgatctcctg cagcatgatg ttcagttcct
      361 ggttgttgcc cagggcactg tcgatcagtg cgtgcaggtc cgggtccttg aagaacgttc
      421 gccaagtgat gttggcgtcg ttggtggtgt ccgtggcacc ggcatagtac gtgcggtcca
      481 ccttccgtgc ctccctcatc ttcagggcgg ggacgcagga cgcgagcagc acggagaccg
      541 tcaggcacgc ggcgaactgg gttttcatcg atcggctcat ggctccggtc ctggttcagt
      601 ccttcttgcg tttcaaaagt tccttcagcc ggtcattcag gtggcggatg gtctccttcg
      661 cggagctgcc ctcctcggca ctgcgtatga attgctccga cacgggttcg tcccgctcgt
      721 cgctgatcag cttgcggccc tggatcatgg tggcgaacac gaagtacaat ccggggatga
      781 tgagcacacc gagcagggtg cccacgaaca tgccgcccag ggccgatgaa ccgatcgtgc
      841 ggttgccgat cgcgcccgcg cccgttgcta ccaccagggg gatcaggccc gcgatgaaag
      901 cgaaggaggt catcaggatg gggcggaacc gggcacgggc cccttcgatc gccgcctccc
      961 gcaccgtggc cccggcgcgt tgcttctgca cggcgaactc cacgatcagc accgcgttct
     1021 tccccagcag gccgatgagc atgatgatac cgatctgggc gtacacatca ttggccaggc
     1081 ccatcatctt caacatcagg aatgatccca gcacacccac gggcagggag agcagcacca
     1141 ccagtggcag tacgaagctc tcgtactgcg cggccagtac caggtacacg aagatgagta
     1201 cgatcaggaa gatgtaaacg gcctcgttcc cccgttgcgc ctcgtcgaag gagagccctt
     1261 cccaggcgat atcatacccc cgcgggagag aggctttggc cacctccttg atggcgttga
     1321 tggcgtcacc gctggtgtag ccggcagcgg ggagccccct gatggcagcg ctgttgtaca
     1381 tgttgtagcg cgtgatctcg ttgggcccca gccgtttctc gaagcgcatg aaggaggagt
     1441 acggcaccat ctcgccctcc tcgttcttca cgaacaggtc ggccaggtcc gacgggtatc
     1501 gccggtactc gggacctgcc tggacgaaga ccttgaaata gcgaccgaaa cggataaagc
     1561 cctgctgata gtcgctgccg atgaggatgt tcaggttctc catggccttg ccgatggaca
     1621 cgcccttctg catggccacc tggttgtcca cgatgagctc gtactgcgga tagttggccg
     1681 catagaaggt gaagagtccg gtcagctcct tccgcttggc cagcgcggcc atgaaatcct
     1741 tgttcacccg gtcgaactcg tggtagtccg tgccattggt cctgtcgagc atgcgcaggg
     1801 agaatccacc ggacgaaccg aagcctggta cggccggcgg ctcgaagtat tcgatcacgg
     1861 caccgaggtc cttggtgagg tgttccagct cctccatcac ctcgttcaca tccttgtggc
     1921 gg
//
LOCUS       DAOQOC010000622         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41113_length_1922_cov_3.408677, whole genome shotgun sequence.
ACCESSION   DAOQOC010000622 DAOQOC010000000
VERSION     DAOQOC010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41113_length_1922_cov_3.408677"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PL106_10025"
     CDS             complement(<1..1577)
                     /locus_tag="PL106_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA33446.1"
                     /translation="MIGRQFLSACTALFVVTSSAQVKPPANGPADKARPVIAFTHATV
                     HPAPGEVIADGTVLIQDDRIIATGPKVSIPVGAVIHDLRGMHMWPGIIEPYEPLGPGK
                     KERDASVDRGDRHWNRAIRAGVRADELFVVDRDRQKALRAQGITTFITHRMDGIARGT
                     GCAVLAAGRSPLEDMILPAASAHFSFRKGTSPDDYPSSLMGAIALLRQTLFDAEWYAA
                     QGRLSEVDADLRALAGQRDLPLVFEASERNDVMRIARIAQEHGLVPIVKEAGDAYARL
                     GDVLATKCPLIVPLALPEAYDVEDPFAALEVSLAKLKHWELAPTNAARIAEAGGSFCF
                     TGNGLKEPGAMWEALRHMVRCGLDSSTAIAALTTVPAKFFRLDDRLGVLKPGMLANLI
                     ISSHHLLDPRNTIHETWVAGSRFTQQTVPLEDPRGTFTLNLRSDILKLKVTGEAASPK
                     ATVQRPDDSTRTTTAAITIERSSVSLLFDGGPFGMNGPVRLNGVIHERGGIWDGQGQL
                     PGGDWAAWSAVRQAGKR"
     gene            1743..>1922
                     /locus_tag="PL106_10030"
     CDS             1743..>1922
                     /locus_tag="PL106_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33447.1"
                     /translation="MAEASLLSLFHSILRWLILLSVSVAGFAALIGYLRKAPIIVWER
                     SVAIIAMVLCHVQLVL"
BASE COUNT          309 a          654 c          600 g          359 t
ORIGIN      
        1 tccctcttcc ctgcctgccg cacggcgctc caggcggccc aatcccctcc cggaagctgc
       61 ccttgtccat cccagatgcc accacgttcg tggatgaccc cgttcagcct gaccggcccg
      121 ttcatgccga atgggccgcc gtcgaacaac agcgagaccg atgagcgttc gatggtgatg
      181 gcggccgtgg tggtccgcgt gctgtcgtcg ggtcgttgca cggtggcctt cgggctggcc
      241 gcctcacccg tcaccttgag cttcaggata tcgctgcgca gattgagtgt gaaggtgccg
      301 cgagggtcct ccaacggtac cgtctgttgg gtaaagcgcg acccggccac ccaggtctcg
      361 tggatggtgt tgcgcggatc gagcaggtgg tgcgaactga tgatgaggtt ggccagcatc
      421 ccgggcttca gcacgcccag ccggtcgtcc agccggaaga acttcgcggg cacggtggtc
      481 aaggcggcga tcgccgtgga actgtccagc ccgcagcgga ccatgtgccg cagggcttcc
      541 cacatggccc cgggttcctt caggccgttg ccggtgaagc agaagctgcc acccgcttcc
      601 gcgatgcgcg ccgcgttggt gggggccagt tcccaatgtt tcagtttcgc caggctcacc
      661 tcgagcgcgg cgaacgggtc ctccacatcg tacgcttcgg gcagggccag cgggacgatc
      721 aacgggcatt tcgtggcgag cacgtcgccc agccgtgcgt acgcatcgcc cgcttccttc
      781 acgatgggca ccagcccgtg ttcctgggcg atgcgtgcga tgcgcatcac atcgttgcgc
      841 tccgatgcct cgaacacgag cggaagatcc cgttgccccg ccagggcccg aaggtccgcg
      901 tccacttcgg aaagcctccc ttgcgccgcg taccattccg cgtcgaacag cgtttgccgc
      961 aacagggcga tggcgcccat gagcgaactc gggtagtcat cgggacttgt ccccttcctg
     1021 aaggagaaat gtgccgatgc cgcgggcagg atcatgtctt ccaagggcga ccttccggcg
     1081 gccagcacgg cgcaacccgt accccgcgca atgccgtcca tccggtgggt gatgaacgtg
     1141 gtgataccct gtgcgcgcaa tgctttctgc cggtcccggt ccaccacgaa caattcatcg
     1201 gcacgaacac cggcacgaat ggcccggttc cagtggcgat cgccccgatc gaccgaggca
     1261 tcccgctcct tcttccctgg gcccaagggc tcgtacggct caatgatccc gggccacata
     1321 tgcatccctc ggagatcatg gatcaccgcg ccaacaggta tgctcacttt cgggcccgtc
     1381 gcgatgatgc gatcatcctg gatgagcacg gtgccatcgg caatgacctc cccgggggcg
     1441 ggatgcaccg tggcatgcgt gaaggcgatc accggacggg ccttgtccgc cggtccattg
     1501 gcgggcggct tcacctgggc cgagctcgtc accacgaaca gtgcggtaca cgctgaaagg
     1561 aactggcggc cgatcatgaa gcgcggcgaa tgtacccttc acctccgctc aagatgacat
     1621 ccggcccgct ggtgcggagg agtccggatg gaatgccact ggccatgcgc gtgtccgact
     1681 tccactgacc ccgaagcggg cgggtccacc gcctaccttt ggcgcgctcc aacctcgtgc
     1741 ccatggcgga agccagcctg ctctcccttt tccacagcat cctgcgctgg ctcatcctgc
     1801 tctcggtgag cgtggcgggt ttcgcagcgc tgatcggtta tttgcgcaag gcgcccatca
     1861 tcgtctggga gcgctccgtc gcgatcatcg ccatggtgct ctgccatgtg caactcgtgc
     1921 tg
//
LOCUS       DAOQOC010000623         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41190_length_1920_cov_4.342627, whole genome shotgun sequence.
ACCESSION   DAOQOC010000623 DAOQOC010000000
VERSION     DAOQOC010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41190_length_1920_cov_4.342627"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="PL106_10035"
     CDS             <1..1557
                     /locus_tag="PL106_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33448.1"
                     /translation="TDGYVDRATADLKSYFVQGAWIGAKSALRFITFRGKEVTYQAWA
                     GVDPAILDTNRTYNPYSYANEVDNYDQAHYQLLIDQRIGASTVLNLTLFRVQGKGYFE
                     QYRPDDDLETYAIAPAIINGDTITTSDIVRRRWLDNSLNGANLSADIKLGEHRLVFGG
                     SASDYRGEHFGELIRARYTGDLHIGDRYYDNDAHKTDLNAFAKFTCAVTPRLDVFGDA
                     QVRSVRYAYLGFNNALDNVSQHIGWTFFNPKAGVLWRVHEGGRAYASCAVGNREPNRE
                     DLQQTTPTSRPTNERLLDYELGYERRNGRASAGVNGFFMDYTDQLVNTGELNDVGSAL
                     RTNVPKSYRAGLELMFAIRPWKRLLWRANATLSSNKVMDFTEYVDDWSTGVQRRVEHG
                     TTDIAFSPAVIASSELGYRFWDRPARGSVELTLITKYVGRQYLDNTSSTDRMLHPYLV
                     NDLRANITWLLKGTKGIDLNLTVRNLFSEMYESNGWAYSYFENDRRKAMIGLFPQAPL
                     NVLGGVSVRF"
     gene            1643..>1920
                     /locus_tag="PL106_10040"
     CDS             1643..>1920
                     /locus_tag="PL106_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33449.1"
                     /translation="MRMPYTLFPVAAFTFATMAFGQANQFDSRIDSYVGLRYPCDGGV
                     QPVLRVQNVGSETMTSCDIDVIKNGLSEGTFNWVLGVPAATNQFRQPA"
BASE COUNT          403 a          585 c          578 g          354 t
ORIGIN      
        1 accgatgggt acgtggaccg tgcaacagcc gacctgaaaa gctacttcgt gcagggggcg
       61 tggatcggtg cgaagagcgc attgcgcttc atcaccttcc gcggcaaaga ggtgacatac
      121 caggcttggg ccggtgtgga tccggccatc ctcgacacca accgcaccta caacccgtac
      181 agttatgcca acgaggtgga caactacgac caggcccact accagttgct gatcgatcaa
      241 cgcatcggcg ccagcacggt attgaacctc accttgttcc gggtacaggg gaagggctat
      301 ttcgaacagt accggcccga tgatgacctg gaaacgtacg ccattgcacc ggccattatc
      361 aatggtgata ccatcaccac ctcggacatc gtacgtcgcc gctggctgga caactccctc
      421 aacggcgcca acctcagcgc tgatatcaag ctgggtgagc atcgcctggt gttcggcggc
      481 agcgccagcg actatcgcgg tgagcacttc ggcgaactca tccgtgcgcg gtacaccggc
      541 gatctgcaca tcggggaccg gtactacgac aacgatgcgc acaaaacgga cctgaacgcc
      601 ttcgcgaagt tcacctgcgc cgtgacccct cggctcgatg tgttcggtga tgcgcaggtg
      661 cgcagtgtgc ggtacgcgta cctcggcttc aacaatgcgc tcgacaatgt gtcccagcac
      721 atcggctgga ccttcttcaa cccgaaggcc ggggtgctct ggcgtgtgca tgagggaggc
      781 cgtgcctacg cgtcatgcgc ggtggggaac agggagccca accgcgagga cctgcaacag
      841 accacgccga ccagccgccc caccaacgaa cgcttgctgg actatgaatt gggctacgag
      901 cgccgtaacg gacgtgccag tgccggcgtg aatggttttt tcatggatta caccgaccag
      961 ttggtgaaca ccggcgagct caatgacgtg gggtcggcct tgcgcaccaa cgtgccgaaa
     1021 agctaccggg ccggattgga gctgatgttc gcgatccgac cgtggaagcg gttgctctgg
     1081 agggccaatg ccacgctcag cagcaacaag gtgatggact tcacggaata tgtggatgac
     1141 tggagcaccg gcgtgcagcg ccgcgtggaa cacggtacca cggacatcgc gttctcacca
     1201 gcggtgatcg cgagcagcga gctgggctac cgcttctggg acagacccgc cagggggtcg
     1261 gtggaactga cattgatcac gaagtacgtc gggcgccagt acctcgacaa cacctccagc
     1321 accgaccgga tgctccatcc gtacctggtg aatgatctgc gcgccaatat cacatggctg
     1381 ttgaaaggca cgaagggtat cgacctgaac ctcacggtgc gcaatctttt cagcgaaatg
     1441 tacgagagca acggatgggc gtacagctac ttcgagaacg accgccggaa ggcgatgatc
     1501 ggcctgttcc cccaagcgcc gttgaatgtg ctcggcgggg tgagcgtgcg gttctgaatc
     1561 ctggggtaac caacgttacc gcttttgggt attgcacgat cgaccgggtc atggctttct
     1621 ttgggacatc aaccgaacga ccatgcgcat gccatacacg ctctttcccg ttgccgcgtt
     1681 caccttcgcc accatggcat tcggccaggc caaccaattc gactcccgga tcgattcgta
     1741 cgtcggcctg cgctacccgt gcgatggcgg tgtgcagccg gtgctgcgcg tgcagaacgt
     1801 gggcagcgag accatgacga gttgcgacat cgatgtgatc aagaacggcc tttccgaagg
     1861 cacgttcaac tgggtgctcg gggtgcctgc agccaccaac cagttccgcc agcccgcctt
//
LOCUS       DAOQOC010000624         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41511_length_1913_cov_2.998385, whole genome shotgun sequence.
ACCESSION   DAOQOC010000624 DAOQOC010000000
VERSION     DAOQOC010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41511_length_1913_cov_2.998385"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PL106_10045"
     CDS             <1..1174
                     /locus_tag="PL106_10045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNA33450.1"
                     /translation="VSDALRNGRSGIVLDQERKDMGFRSGLTGRVWVPDVKDRLNRRQ
                     RIGMAQQVFYAFEATEQAMKQAGIDTAWCERNEVGIIFGNDSSALAVTESVDTIRAKK
                     DTTLLGSGAIFQGMNCTVTMNLSTVFKLKGVNFTLSGACASGSHAIGIGYLLIKQGLQ
                     KMVLCGGAQEVNPFAFGSFDGIGAFSVREGDPTGASRPFDKDRDGLVPSGGAAALVIE
                     SLESARARGATILAEITGYGFSSNGAHISDPDLDGQVRALHMALGMAGIPAEKVDYIN
                     AHATSTPVGDRIEAQALHAVFGQRRPWVSSTKSMTGHECWMAGASEVVYSMLMLLDGF
                     LAPNLNLQAPDEDSAKLNLVRETRNETIDTFLSNSFGFGGTNSTLIIQRFQDSQTA"
     gene            1171..1431
                     /locus_tag="PL106_10050"
     CDS             1171..1431
                     /locus_tag="PL106_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNA33451.1"
                     /translation="MSNSTRQEIEARTIKFLSEEFEVDPATIKPDAPLKETLALDSLD
                     YVDLVVVIEENFGFKVNAEDFMKLVTFKDFYDHIEAKTTAQV"
     gene            1469..>1913
                     /locus_tag="PL106_10055"
     CDS             1469..>1913
                     /locus_tag="PL106_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNA33452.1"
                     /translation="MGASWTGQSKGTPLGYRIFILVMRTLGVRAAHGMLYLVVPWYVL
                     TGRTSNRALRGYFRRLRATDPRIKGLSLAGSYRAFGRTIIDKVALRAGLEDRYTWAKT
                     GRQHLVAMLEAGQGGVLISAHLGNWELASHMLHDSPGKISVVLQDG"
BASE COUNT          373 a          593 c          615 g          332 t
ORIGIN      
        1 ggtatccgat gccctgcgga atggccgttc cggcatcgtc ctcgaccagg agcgcaagga
       61 catgggcttc cgcagcgggc tgacgggccg ggtatgggta ccagatgtga aggatcgatt
      121 gaaccggcgc cagcgcatcg gcatggcgca gcaggtgttc tacgcgttcg aggcgaccga
      181 gcaggccatg aagcaggccg gtattgatac cgcatggtgc gaacggaacg aagtgggcat
      241 catcttcggc aacgacagct ccgcgctggc cgtgaccgaa tcggtggaca ccatccgtgc
      301 caagaaggac accaccctgc tgggctccgg cgccatcttc caggggatga actgcacggt
      361 gaccatgaac ctgagcaccg tgttcaaact gaaaggcgtg aacttcaccc tcagcggcgc
      421 ctgtgccagc ggttcgcacg ccatcggcat cggctacctg ctcatcaagc agggcttgca
      481 gaagatggtc ctgtgcggcg gggcgcagga ggtgaacccc ttcgccttcg gcagtttcga
      541 cggcattggc gcgttcagcg ttcgcgaggg cgaccccacc ggggcttcgc gccccttcga
      601 caaggatcgc gatgggctgg tgcccagcgg tggtgcggca gcgctggtga tcgagagcct
      661 cgagagcgcc cgggcgcgcg gggccaccat cctggccgag atcaccgggt acggcttctc
      721 ctccaacggg gcccacatct ccgaccccga cctcgacggc caggtgcggg ccctgcacat
      781 ggccctggga atggcgggca tccccgctga aaaggtggac tacatcaacg cccacgccac
      841 cagcaccccg gttggcgacc gcatcgaggc acaggctttg catgccgtgt tcgggcagcg
      901 caggccatgg gtcagttcca ccaaaagcat gaccggccac gagtgctgga tggccggcgc
      961 cagcgaagtg gtgtactcca tgctgatgct ccttgacgga tttctggcac cgaaccttaa
     1021 tttgcaggcg ccggacgagg acagcgccaa attgaacctg gtccgtgaaa cacggaacga
     1081 gaccattgac accttcctct ccaactcgtt cggcttcggc ggaacgaact ccacactgat
     1141 cattcaacgt ttccaggaca gtcagaccgc atgagcaatt ctacccgtca agagatcgag
     1201 gcccgcacca tcaagttcct cagcgaggag ttcgaggtgg atcccgccac gatcaaaccc
     1261 gatgcaccgt tgaaggagac gctggccttg gacagcttgg attatgtgga cctcgttgtg
     1321 gtgatcgagg agaacttcgg cttcaaggtg aacgccgagg acttcatgaa gctggtgacc
     1381 ttcaaggatt tctacgatca catcgaggcg aagacgaccg cacaggtctg aggcctgtca
     1441 gcgtacacgg cccgcaacac cacccccgat gggcgcttcg tggacaggcc agagcaaggg
     1501 cactccgctc ggataccgca tcttcatcct ggtcatgcgc accctcggcg tgcgtgctgc
     1561 gcatggcatg ctgtacctcg tggtgccctg gtacgtgctc accggccgca cgagcaaccg
     1621 ggccctgcgc ggctacttcc ggcggctgcg ggcaactgac ccccgtatca agggattatc
     1681 gctggccggg tcgtaccgtg cgttcggccg caccatcatc gacaaggtgg ctttgcgggc
     1741 ggggttggaa gatcggtaca cctgggcgaa gacgggtcgc cagcacctgg tcgccatgct
     1801 ggaggccgga cagggtggtg tgctgatcag tgcacacctg ggaaactggg agctggcgag
     1861 ccacatgctg cacgactcgc ccggaaagat cagtgtggtg ttgcaggatg gcg
//
LOCUS       DAOQOC010000625         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41521_length_1913_cov_2.525296, whole genome shotgun sequence.
ACCESSION   DAOQOC010000625 DAOQOC010000000
VERSION     DAOQOC010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41521_length_1913_cov_2.525296"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1913)
                     /locus_tag="PL106_10060"
     CDS             complement(<1..>1913)
                     /locus_tag="PL106_10060"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNA33453.1"
                     /translation="LDYDKTIALVDKYFGKWKRKEVPGFSFAPEDPINAPIQAEVFGP
                     DREWVSLAWRFNGTKSDDPIMLELIAGILSNGKAGLFDLNLLQEQKVLGAQAFSGEQA
                     DYSEFGMRIQPREGQTLEEARDLALGQLEALKRGEFDDWLIEAVVNDNKQQQIRFWNE
                     HNSLRASAMTDAFILRKDWQRMVDLYDRMGRITKQQVMDFAKARFNNNYVCVFKRTGE
                     ASGRHKVTKPKITPIDIKRDTKSAWRLEWEKVLPATMIPEFVDFNTAISRKSLTSGVE
                     LACVKNPSNDLFSLNYIVDMGSLNDRELRVAIDYLDYLGTSKYSPVDLKKELFKLGLD
                     ISVSAGDDRSVVTLSGLDKHLEEGIQLLEHVLSDAQPNKEALPGLIADIRQKRQDQLK
                     SKGGLLYSGLLNMARYGGKNPLNDILTNEQLAEFGPQQLIDRIHGLTGWKHRIFYYGK
                     KGIDEMAPLLTKYHQVPAVLKDCPPERVYPELPTTANTVLFTEYDMVQTEMVLNSKAG
                     LFDAAKLPFASLFNEYFGSGLSSIVFQEIREAKALAYGANASYTSPAKKADAHYVRAF
                     IGTQADKLPDALDAMLKLMNEMPMAEAQFEGAKTAALKVIASTRITKENIYWQWEAAR
                     RRGLDHDVRRDIY"
BASE COUNT          321 a          551 c          594 g          447 t
ORIGIN      
        1 gtagatatcg cgtcgtacgt cgtggtccaa cccgcgtcgc cgcgccgcct cccattgcca
       61 gtagatgttc tccttggtga tccgggtgct ggcgatcact ttcaaagccg ctgttttggc
      121 accttcgaac tgggcttcgg ccatgggcat ctcattcatc aacttgagca tggcatcgag
      181 cgcgtccggc aatttgtcgg cctgggtgcc gatgaaggcc cgaacgtaat gggcatcggc
      241 cttcttggcc gggctggtgt aactggcgtt ggcaccgtag gccagggcct tggcttcgcg
      301 gatctcctgg aagacgatgc tgctgagtcc gctgccgaag tactcgttga agagcgatgc
      361 gaagggcagt ttggccgcat cgaaaagacc cgctttgctg ttgagcacca tctcggtctg
      421 caccatgtcg tactcggtga aaagcacggt gttcgccgtg gtgggcagtt cgggatacac
      481 gcgctcgggc gggcagtcct tcagcacggc gggcacctgg tggtacttcg tgagcagcgg
      541 ggccatttca tcaatccctt tcttcccgta ataaaaaata cggtgcttcc agccggtgag
      601 gccgtggatg cggtcgatga gttgctgtgg tccaaactcg gccagttgct cgttggtcag
      661 gatgtcgttc agcgggttct ttccgccgta tctggccata ttgagcaaac cggaatagag
      721 caggccgccc ttgctcttca attggtcctg ccgtttctgg cggatatcgg cgatcaatcc
      781 cggcagggcc tctttgttgg gctgcgcgtc ggagagcacg tgttccagca actggatgcc
      841 ctcttccaga tgcttgtcaa gcccgctcaa ggtcaccacc gagcggtcat ccccggcgga
      901 gacggagatg tcgagtccca gcttgaagag ttccttcttg aggtcgacag ggctgtactt
      961 ggatgtaccg agataatcga ggtaatcgat ggccacgcgc agttcccgat cgttcaagga
     1021 ccccatgtcg acgatgtagt tcaagctgaa caggtcattg ctcgggttct tcacgcaagc
     1081 caattcgaca ccggaggtca gcgatttgcg gctgatggcg gtattgaagt ccacgaattc
     1141 cgggatcatg gtcgcgggca gtaccttctc ccactcgagt cgccaggcgc tcttggtgtc
     1201 acgcttgatg tcgatggggg tgatcttggg cttggtcacc ttgtggcgac cggaggcctc
     1261 gcccgtccgc ttgaagacgc acacgtagtt gttgttgaag cgcgccttgg cgaagtccat
     1321 cacctgctgt ttggtgatgc ggcccatgcg gtcgtacagg tccaccatgc gttgccagtc
     1381 cttgcgcagg atgaacgcat cggtcatggc actggcccgg aggctgttgt gctcattcca
     1441 gaagcggatc tgttgttgct tattgtcgtt caccacggcc tcgatcagcc aatcgtcgaa
     1501 ctcgccgcgc ttgagggcct ccagttggcc gagggccagg tcgcgcgcct cttccagcgt
     1561 ctgtccttcg cggggctgga tgcgcatacc gaattcgctg tagtccgcct gttcgcccga
     1621 gaaggcctgg gcacccagca ccttctgctc ctgcaacagg ttcaggtcga agaggccggc
     1681 cttgccgttg ctcaggatgc ccgcgatcag ttcgagcatg atggggtcat cgctcttggt
     1741 gccgttgaag cgccaggcca ggctcaccca ttcgcggtcg ggaccgaaga cctcggcctg
     1801 gatcggtgcg ttgatggggt cctcaggtgc gaaggagaag ccgggcacct ccttgcgctt
     1861 ccacttcccg aagtacttgt cgacgagcgc gatcgtcttg tcgtagtcca ggt
//
LOCUS       DAOQOC010000626         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41562_length_1912_cov_2.934303, whole genome shotgun sequence.
ACCESSION   DAOQOC010000626 DAOQOC010000000
VERSION     DAOQOC010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41562_length_1912_cov_2.934303"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PL106_10065"
     CDS             complement(<1..521)
                     /locus_tag="PL106_10065"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNA33454.1"
                     /translation="MTTLDLTSRLTLPVVAAPMFIISGTELVIECCKNGIVGTFPALN
                     GRTSEDFERMLARITGELERHEQTTGRKAAPFGVNIIVHPTNPRIMADLQLCAKYKVP
                     LIITSLGAVKELVSAVHDHGGLVFHDIVKKRHAEKAAEAGVDGLILVAAGAGGHAGTA
                     SPFALVAEIRSFYT"
     gene            complement(615..1415)
                     /locus_tag="PL106_10070"
     CDS             complement(615..1415)
                     /locus_tag="PL106_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33455.1"
                     /translation="MRTPPGQLLALVLPLGIMAQPTEKTISTDSGRVELHYFTSGEIS
                     TKAWMDKDDRWGHSWAYKRSGEVIFDGQTRKVGGHASIRFWYYPSGAVSKVETSDAPD
                     GGIQWYESTTTFDEDGQRTGFQESGQDDNGPITRPTVTRIPPAVVQPVVPETVYEQRL
                     FVNELFVVNPTKWTCAVDIIVDDPSPALRGGHTTIGPRDTLRIGSYSIGERFSPPDEH
                     VRVFLSRKKGKSRWYRLANYRLHEVQVNPEHRRYYRVVDGWITTKRIW"
     gene            1512..1709
                     /locus_tag="PL106_10075"
                     /pseudo
     CDS             1512..1709
                     /locus_tag="PL106_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
BASE COUNT          317 a          628 c          598 g          369 t
ORIGIN      
        1 gtgtagaagc tccggatctc cgcgaccagg gcgaagggac tggccgtgcc cgcatgcccc
       61 ccggccccag cggccaccag gatcaacccg tccacaccgg cctcggcggc cttctcggca
      121 tggcgcttct tcacgatgtc atggaatacc agtccgccgt gatcgtgtac ggcgctcacc
      181 agctctttca ccgcacccag cgacgtgatg atcagtggca ccttgtactt ggcgcacagc
      241 tgcaggtcgg ccatgatgcg cgggttggtc ggatgtacga tgatgttcac cccgaagggc
      301 gcggctttcc gcccggtggt ctgttcatgc cgttccaact caccggtgat ccgcgccaac
      361 atgcgctcga agtcctcgct ggtgcggcca ttgagcgcgg ggaaggtgcc gacgataccg
      421 ttcttgcagc attcgatcac cagctcggtg cccgatatga tgaacatggg cgcagcgacc
      481 acgggaaggg tcagtcgcga agtgaggtcg agggtggtca tgatcgggtg tgcgggaccc
      541 gggcaagata ggtgcgggtc ccgtctacgg gaggcatcct ggtggggcga tccggtccgg
      601 aaggcccttc ccgatcacca gatccgcttg gtggtgatcc atccatccac cacgcggtag
      661 taccgcctgt gctccgggtt cacctgcact tcgtgcaacc gatagttcgc cagcctgtac
      721 catcggctct tgcccttctt gcggctcaag aagacgcgga cgtgctcatc cggtggtgag
      781 aatcgttcgc cgatgctgta gctgccgatc cgcagggtgt cccgcggccc gatggtcgta
      841 tgcccaccgc gcagggcagg tgaggggtcg tccacgatga tgtcgaccgc gcaggtccac
      901 ttggtgggat tcaccacgaa caattcgttc acgaacaggc gctgttcgta cacggtctcc
      961 ggtacaacgg gttgcactac cgcggggggg atcctggtca ccgtgggccg cgtgatcggt
     1021 ccgttatcgt cctgcccgga ttcctggaac cccgtccttt ggccatcctc atcgaaggtg
     1081 gtggtgctct cgtaccattg gatccctcca tcgggcgcat cgctggtttc cactttgctc
     1141 accgcaccgc tggggtagta ccagaaccgg atcgacgcat ggccgccgac cttcctggtc
     1201 tgcccgtcga agatcacctc gccgctccgt ttgtacgccc agctatggcc ccagcgatca
     1261 tccttgtcca tccacgcctt cgtgctgatc tcgccggaag tgaagtagtg cagctccacg
     1321 cgcccactat cggtggagat cgtcttctcc gtgggttgag ccatgatgcc gaggggaagg
     1381 accagtgcca atagctggcc gggaggggtg cgcatgcctc ggtcctgcca aaattcaggc
     1441 caacgatccg cgtgctgtcc ggcaaagcgg gatattcgcc catcccaacc gttcccgtgg
     1501 ccaagaagaa gatcaagaag ctgctcgtcg ccaaccgtgg cgagatcgcc ctgcgcgtga
     1561 tgcgctccgc ccgcgagatg ggcctcagca ccgtggcggt gttcagcgag gccgaccgca
     1621 acgcgccatt cgtgcgtttc gccgacgagg ccgtgtgcat cggcccgcct gccagcaagg
     1681 aaagctacct ggtggtggac aagctggtga actacccgca ctccatccag cagtagcggg
     1741 cgggatggcc tcgaccttcg actccgtccg cgactacatc ggggcgctgg agcgccgcgg
     1801 ccggctgttg cgcctgccgg agatggacca ggaccgctac gaggccactg ccttcgccta
     1861 ccgcctcatc gagcgccttg gcatcgagca ggcgccggcc ttcctcatcg ag
//
LOCUS       DAOQOC010000627         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41566_length_1912_cov_2.745827, whole genome shotgun sequence.
ACCESSION   DAOQOC010000627 DAOQOC010000000
VERSION     DAOQOC010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41566_length_1912_cov_2.745827"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..>1912
                     /locus_tag="PL106_10080"
     CDS             386..>1912
                     /locus_tag="PL106_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33456.1"
                     /translation="MNCRPYSTPPCRTWGWLKSGLLGAAMVLFGTAQAQVSNYAFSQS
                     VGTYTATSGAATQIIAPNIDDGNSAATNIGFSFTYNGSPFTQFVANSNGHIRLGGTAP
                     TGSYTPLSTASNTNAISLLGRDGRSIDGVFVETTGAPGSQVCVIEFRTQQLQYNVTTS
                     RMTGQIRLYEASGVVEMVYGTGIQGATYTPQVGLRGGTGTTDFNNRTTTTDWAATTAG
                     ATSSATCTYSTTSYPASGQTFTWTPPVPCSSVNAGAAVASVTSGCGTVASSTLSLSGA
                     TSGVTGLTYQWYSGPVGGPYLTPLGTASTESVNGLVSTTAYVCTVTCTNGGATATSSE
                     AVITVNTVPTVVASATDPGPFCGTANTDLNATGADTYTWSPATGLSAATGTPVSCTAT
                     STTTYTVTGTVTATGCTGNTTIVVTVNPAPIISSVTANPNPICFNGSSQLDVLATVPA
                     PVSDYSFASTPGTFTSIVGGPGTVQIATASDDSNFGPYPLGFSFVFNGTTFTTAGVNA
                     N"
BASE COUNT          383 a          672 c          510 g          347 t
ORIGIN      
        1 gattcgaggc ccgacaacct cacctggcct cccggaacgg cccgtatggc gcctgttctg
       61 gtgctggatc cgggcgcggg cacggccccg gccgcctgtt ccgaaaagtt atcaacatat
      121 ctgaccttat agcttgcgcc ctacggtcca gccccgtaag cttgccaaac ccataagccg
      181 gaacttccga ccggcacacc ctgttcgacc ggacccgtgt ccaccagctc caacgcaccg
      241 aacgccccgt acacgcccct ccaactcagc cggggtcgca ctaaagggcg cctctccgta
      301 ccttttcatt gggatattat ccagccctga ggatcgtgtc cctcgccccg cgaacaagaa
      361 aacccaaacc caaaaccctc gatccatgaa ctgtcgaccc tactcgacac caccctgccg
      421 aacgtgggga tggctcaagt ccggactgct cggcgccgcg atggtgctgt tcggcacggc
      481 gcaggcgcag gtgtccaact acgccttctc ccaatcagtg ggcacctata ccgctacctc
      541 cggagcagcc acgcagatca tcgccccgaa catcgatgat gggaactcgg ccgcgacgaa
      601 catcgggttc tcgttcacct acaacggaag cccgttcacc caattcgtgg ccaactccaa
      661 tggccacatc cgcctggggg gcactgcacc gaccggcagc tatacgccgc tttccacggc
      721 ctccaatacc aatgcgatct ccctgctcgg gcgtgatgga cgatcgatcg atggggtctt
      781 tgttgagacc acaggcgcac cgggatccca ggtctgcgtg atcgaattcc ggactcaaca
      841 attacagtac aacgtgacca cgagccggat gaccgggcag atccggttgt acgaggcatc
      901 tggcgtggtt gaaatggtgt acggcacggg tatccaaggc gccacctaca ctccgcaggt
      961 cggcctgcgc ggtggtacag gaaccactga tttcaacaat cggacaacaa caacggactg
     1021 ggccgcgacc acggcaggtg caacgagtag tgccacctgc acatactcca ccacatcgta
     1081 ccccgcttcg ggacaaacct tcacatggac gcctccggtg ccatgttcca gcgtcaatgc
     1141 aggagcagcg gtagcctctg tgaccagcgg ttgtggcact gtggcctcat ccaccctttc
     1201 cctttcagga gctacctcgg gtgttacagg cctcacctac cagtggtatt caggcccggt
     1261 cggtggtcca tacctcaccc cgctgggcac ggcttccacc gagtcggtga acggccttgt
     1321 ctccaccacc gcgtatgtct gcacggtgac ctgcaccaac ggaggtgcca cggcgacctc
     1381 cagcgaagcg gtgatcacgg tgaacaccgt gcctacggtc gtcgcatcgg ctacggatcc
     1441 tggcccattc tgtggtacgg cgaacacgga cctgaatgcg accggggccg atacctatac
     1501 ctggagtccg gcgacgggtc ttagcgccgc gaccggtacc cctgtatcct gcacggccac
     1561 cagcaccacc acctacaccg tgacgggtac cgttacggcc actggatgta ccggcaacac
     1621 cacgatcgta gtaaccgtga acccggcccc gatcatttcc tccgtgacgg ccaacccgaa
     1681 ccccatctgc ttcaacggca gcagccagtt ggatgtgttg gctaccgtcc ccgccccggt
     1741 gagcgactac agcttcgcga gcacgccggg caccttcacg tccattgttg gcgggcctgg
     1801 aacggtccag atcgccactg cctcggatga ctcgaatttc ggaccctacc ccttgggctt
     1861 cagcttcgtg ttcaacggca cgaccttcac caccgccggt gtgaacgcga ac
//
LOCUS       DAOQOC010000628         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41740_length_1907_cov_4.295896, whole genome shotgun sequence.
ACCESSION   DAOQOC010000628 DAOQOC010000000
VERSION     DAOQOC010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41740_length_1907_cov_4.295896"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..851)
                     /gene="folE"
                     /locus_tag="PL106_10085"
     CDS             complement(168..851)
                     /gene="folE"
                     /locus_tag="PL106_10085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNA33457.1"
                     /translation="MMRKRKPQLPGIGKRKVSAPNIGPEDEVDGYTRIDVYDDRTIAR
                     IGAHYGDILKSIGEDPKREGLQKTPERVAKALQYLTHGYDLDPAAILRSALFHENYSQ
                     MVLVKDIEVYSLCEHHMLPFFGKAHVAYIPDGRITGLSKIPRVVDAFARRLQVQERLT
                     NEIRDCVQDTLRPLGVAVVMECDHLCMRMRGVQKQNSVTTTSAFTGIFLSDHRSREEF
                     IKLISAKLH"
     gene            complement(848..1264)
                     /locus_tag="PL106_10090"
     CDS             complement(848..1264)
                     /locus_tag="PL106_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNA33458.1"
                     /translation="MSLVHITRRERFSAAHKLSRADWSPERNLEVFGKCSSPNWHGHN
                     YELWVTVKGEPSTDTGFVVDLSELGRIIHDRVIRHVDHKNIDVDVEFMKGRLSSTENL
                     AIAIWEQLSGPISGIGCALHRIRLKETENNEVEYYG"
     gene            1359..>1907
                     /locus_tag="PL106_10095"
     CDS             1359..>1907
                     /locus_tag="PL106_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA33459.1"
                     /translation="MIIVTGAAGFIGSALVGELLRQGWADIVAVDDFSHPDKAPNLEG
                     KTLTARVDRMDFPSWLDANESRVQFIFHLGARTDTTLFDKAVFDELNIRYSQHVWDRC
                     VRYGIPLVYASSAATYGDGTLGYGDTDDKLPYRLKPLNPYGDSKNDFDKWALEQERKP
                     YFWAGLKFFNVYGPNEYHKGRMA"
BASE COUNT          348 a          608 c          556 g          395 t
ORIGIN      
        1 ggaactgact gccctggccg gggaatacgt atgcggtcat gacgatgatc ggggattgtg
       61 ttcggatggg gtgaccagtg ggccctgggg ttggtccgtt cgttctatcg ccgacgaata
      121 tcgtgcgcct cttgtgaaca atgcgcaacg gcccgcgccg ctaccggtca gtgcagcttc
      181 gcgctgatca gcttgatgaa ttcctcgcgc gagcggtggt cgctgaggaa gatgcccgtg
      241 aacgcgctcg tggttgtcac gctgttctgc ttctgcacac cgcgcatgcg catgcacaag
      301 tggtcgcact ccatcaccac ggccacgccc aacgggcgca acgtgtcctg aacgcaatcc
      361 cggatctcgt tcgtaagacg ctcctgcacc tgcagtcgcc tggcgaacgc atcaaccacc
      421 cgggggatct tgctcagccc ggtgattcgc ccatcgggga tgtaggccac atgcgccttc
      481 ccgaagaagg gcagcatgtg gtgctcgcac aagctgtaca cctcgatgtc cttcaccagt
      541 accatctggc tgtagttctc gtggaacaac gcgcttcgca gtatggcggc cgggtccagg
      601 tcataaccat gcgtgaggta ctggagcgcc ttcgccacac gctccggcgt cttctgcaag
      661 ccttcccgct tcggatcctc gcctatgctt ttcaggatat cgccgtaatg cgcaccgatg
      721 cgggcgatgg tcctgtcgtc gtacacatca atgcgcgtgt atccatccac ctcgtcctcg
      781 ggcccgatgt tcggtgccga taccttcctc ttcccgatcc cgggcagttg gggctttctc
      841 ttgcgcatca tccgtagtac tccacctcgt tgttctccgt ctccttcaac cgtatcctgt
      901 gaagggcgca gccgatcccg gagatggggc cgctgagttg ctcccagatc gcgatggcca
      961 ggttctcggt gctgctgagg cggcctttca tgaactccac gtccacgtcg atgttcttgt
     1021 ggtccacatg acggatcacg cgatcatgga tgatcctccc caattctgaa agatccacca
     1081 cgaaaccggt atcggtcgaa ggctccccct tcaccgtgac ccacaactcg tagttgtgcc
     1141 cgtgccagtt cgggctggag cacttgccga agacctccag gttgcgctcg ggactccaat
     1201 ccgcccggct cagtttgtgc gcggcgctga aacgctcgcg acgggtgatg tggacgaggg
     1261 acatgattat tgaagggctg cgaaaataca tgctcagagg cagcaaccgg accacggcaa
     1321 cggcggtttc ccgctcccac ccccgtatgt ttgtccgcat gatcatcgtc accggtgcgg
     1381 cgggcttcat cggcagcgcg cttgtgggag agttgctccg ccagggatgg gccgacatcg
     1441 ttgccgtgga cgacttctca cacccggaca aggccccgaa cctcgaaggc aagaccctca
     1501 ccgcccgggt ggaccgcatg gacttcccct cctggctcga tgccaacgag agccgggtcc
     1561 agttcatctt ccacctcggt gccagaacgg acaccacgct ttttgacaag gccgtcttcg
     1621 atgagctgaa tatccggtac agccagcacg tgtgggaccg ctgcgtgcgg tacggcatcc
     1681 cgttggtgta cgccagcagc gcggccactt acggggatgg caccctcggt tacggggata
     1741 cggacgacaa gctgccctac cggctgaaac cactgaaccc ctacggcgac agcaagaacg
     1801 acttcgacaa gtgggccctg gagcaggagc ggaaaccgta cttctgggcc ggcctcaagt
     1861 tcttcaatgt gtacggcccg aatgaatacc acaaggggcg tatggcc
//
LOCUS       DAOQOC010000629         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_41921_length_1904_cov_2.513250, whole genome shotgun sequence.
ACCESSION   DAOQOC010000629 DAOQOC010000000
VERSION     DAOQOC010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41921_length_1904_cov_2.513250"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PL106_10100"
     CDS             complement(<1..519)
                     /locus_tag="PL106_10100"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNA33460.1"
                     /translation="MQLPAVTDTIHSVSMATFALPPKRPHDPMETFISDISKRPFPVR
                     ERVSAKSIQKSLLSFIRQEHPTFSDSGHLSLNELNTFRRQYISTFLEQEINELPTLQA
                     AVQSASKEDDLLTKQLESEGSTKLTFGQSLADKVATFGGSWTFILFFGGFLLLWIATN
                     AFVLMNKGFDPYP"
     gene            635..>1904
                     /locus_tag="PL106_10105"
     CDS             635..>1904
                     /locus_tag="PL106_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33461.1"
                     /translation="MRKLYVSLCLLLASALPAVAQFNVTFQVDMNTQAVVDDTVSVAG
                     NFQVAAGFASDWTPGITRLTDANADGVYDLTVQLPAGSYEYKFLNGAAWGTDEGVPGA
                     CNVNGNRGMTISGDTTLSLVCFGSCTACPVGVDTVDVTFQVEMRYLTLASMVTIAGDF
                     QNDVPGAGWSDWTPGITELTDPNNDSVYTLTVRLPEGTYAYKYINGTAWGQDESVPGA
                     CAVNNNRELIVTGPGPITVPVHCFASCSACVAPLPAINVTFRVDMSNEIVNSGGVFVA
                     GSFQNPAWVKDTLLMTDVDADGIYEHTEAIVPNEYQFKYYNGPGGDADGETADFLALG
                     CGVSNGLGGFNRLLNIVGRTTDTILPVWEYNSCTTVATGVAEQEGTTFSVYPNPFSST
                     TVIELNRWERSAFDLRVVSVTGQVMMERNGL"
BASE COUNT          385 a          555 c          549 g          415 t
ORIGIN      
        1 gggatagggg tcgaagccct tgttcatcag cacgaaggca ttggtcgcga tccagagcag
       61 caggaagccg ccgaagaaga ggatgaaggt ccacgagccg ccaaaggtcg cgaccttgtc
      121 cgcgagactc tgtccgaagg tcaacttggt cgagccctcg gattccaatt gcttggtgag
      181 gagatcgtct tccttggagg cgctttggac ggcggcttgg agggttggca gctcgttgat
      241 ctcttgctcc aggaaggtgc tgatgtattg tcggcggaag gtgttgagct cgttcagaga
      301 caggtgtccg ctgtcagaaa aggtgggatg ctcctgccgg atgaacgaaa gcaaggattt
      361 ctgaatggac ttggcggata cacgttcgcg caccgggaag ggccgcttgg agatgtcgct
      421 gatgaaggtt tccatgggat catgcggcct tttgggcggc agtgcaaagg tagccattga
      481 aacgctatgg atggtgtcgg tgacagcggg cagttgcatg gaagaatctg ggacttcgat
      541 gtaacttttt cccttcccaa ttgtgaatct tccgtaactt agtgtcatct aaacactttg
      601 ttagtattgg actcaattgt acaaggttaa ggttatgaga aaactctacg tttcactttg
      661 tctattgctc gcttcggcat tgcccgcagt cgcgcaattc aatgtcacct ttcaggtgga
      721 catgaacaca caggccgtgg tggatgacac cgtgtctgtg gccggtaact tccaggtggc
      781 tgcgggtttc gcatctgact ggacacccgg tatcacacgc ctcaccgacg caaatgccga
      841 cggggtctat gacctcacgg ttcagcttcc cgcaggctcc tacgagtaca agttcctcaa
      901 cggcgctgct tggggcaccg acgaaggcgt tcccggtgct tgcaacgtca atggcaaccg
      961 tggcatgacc atcagcggtg acaccaccct gagcctcgtc tgctttggca gctgcaccgc
     1021 ttgccccgtg ggcgtggaca ccgtggacgt gaccttccaa gtggaaatgc gttacctcac
     1081 gctcgcctcc atggtgacca tcgctggtga cttccaaaac gacgtccccg gtgctggttg
     1141 gagcgactgg actcccggca tcacggaact gaccgacccc aacaacgaca gcgtctatac
     1201 cctgacggtc cgcctccccg aaggcaccta cgcttacaag tacatcaacg gtactgcttg
     1261 gggacaggac gaaagtgttc ccggtgcttg cgctgtgaac aacaaccgcg aactgatcgt
     1321 gaccggcccc ggtcccatca cggttcctgt gcactgcttc gcttcttgca gcgcttgcgt
     1381 ggctccgctt cccgcgatca acgtgacctt ccgtgtggac atgagcaatg agatcgtgaa
     1441 ctccggtggc gttttcgtgg ccggctcctt ccaaaaccct gcttgggtga aggataccct
     1501 cctcatgacc gacgtggacg ctgacggcat ctacgagcac actgaggcca tcgtcccgaa
     1561 cgagtaccaa ttcaagtact acaacggccc cggcggcgac gctgacggcg aaactgccga
     1621 cttcctcgcc ctcggctgcg gtgtttccaa cggcctcggc ggcttcaacc gtctcctgaa
     1681 catcgtgggc cgcaccaccg acaccatcct gcctgtgtgg gaatacaact cctgcaccac
     1741 ggtggctacc ggcgtggctg agcaagaagg caccaccttc agcgtgtacc ccaacccctt
     1801 cagcagcacc actgtgatcg agctgaaccg ttgggagcgt agcgcattcg acctccgcgt
     1861 ggtttccgtg accggccaag tcatgatgga gcgcaatggc ctcc
//
LOCUS       DAOQOC010000630         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42001_length_1902_cov_3.029237, whole genome shotgun sequence.
ACCESSION   DAOQOC010000630 DAOQOC010000000
VERSION     DAOQOC010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42001_length_1902_cov_3.029237"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PL106_10110"
     CDS             <1..682
                     /locus_tag="PL106_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33462.1"
                     /translation="VQPQHSPFLYMGQTTAEASRAIGNIIGSTTWEGSQCMVLLDAHA
                     PLLFANNLVHWTRNLPSASAVTLVGNTVDGITTAGMELRNNTIMRTNGTTQPWLVEGV
                     PDSFPFTAMLTNNLVVGGAAFTVPTGTANLFSDTFNTLTMDFASYNANTGQPLPGSLA
                     MDAGDPDVAHLDLDLSRNDAGCWGGSFSHANFQPAPDSGALVGLVTAPRRFGQLTPMS
                     FTATGSDR"
     gene            688..>1902
                     /locus_tag="PL106_10115"
     CDS             688..>1902
                     /locus_tag="PL106_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33463.1"
                     /translation="MRTLHALLAVVLFVALRAQVPVLQQAEYFWDSDPGPGNGIAMSA
                     MDGTFDRAFEQVLAYAIPPSPGLHKLCVRVKGSDNSWGPVFSTIMDVVAMRDARVITA
                     ECFWDADPGPGSGTPMLAFDGNFNSAFEQLSGNLTAPSAGLHRLSIRVRGVDNTWGPV
                     FSTIVDVVNARQVRITSAEYFWDTDPGAGNGQPMIAFDGAFNSVLEQMSATVSLTALS
                     VGPHVLHLRAKGADNSWGPVHRSVVHVDPQPEVTVSFDLKVALQGCMGTAALMNNGLR
                     NAGLVPLAEPYSALGHDLGLSAGATTTSAVLSVMFPPGASVVDWILVEFHPALAPSQI
                     TAAMPLLLRRGGTVAQTDGGYPFLITLPAASYYVVVRHRNHLPVATESPVAISTNGAT
                     VAIDFTAAAVQAF"
BASE COUNT          352 a          608 c          582 g          360 t
ORIGIN      
        1 ggtgcagccg cagcacagcc ccttcctgta catgggccag accaccgccg aggcgagccg
       61 cgcgatcggc aacatcatcg ggagcacgac ctgggaaggc agtcagtgca tggttctgct
      121 ggacgcccat gccccgctgc ttttcgccaa taacctggtg cattggacac ggaacctgcc
      181 ttcggcttcc gcggtcacct tggtgggcaa caccgtggat ggtatcacga cggcaggcat
      241 ggagctgcgg aacaacacca tcatgcgcac caacggcact acgcaaccct ggctggtgga
      301 gggggtgccg gattccttcc cattcaccgc catgctcacc aacaacctgg tggttggagg
      361 tgccgcattc acggtaccaa ctggcacagc caacctgttc tccgacacgt tcaacacgct
      421 gaccatggac ttcgcctcgt ataatgcaaa caccggccag ccactgccgg gttcactggc
      481 gatggatgcg ggcgaccccg atgtcgcaca cctggatctg gacctctcgc gcaacgatgc
      541 gggttgctgg ggcggtagtt tcagccatgc caatttccag ccggcacctg atagtggtgc
      601 gctcgtcggc ctggtcacgg cgccccgccg cttcggccaa ttgacgccca tgtccttcac
      661 cgccaccgga tccgatcgct gaacaacatg agaaccctgc atgccctact tgccgtcgta
      721 ctgttcgttg cgctccgggc ccaagtaccc gtactacagc aggccgagta cttctgggac
      781 tcagaccccg gcccgggcaa tgggatcgcc atgagcgcca tggacggcac ctttgaccgt
      841 gcgttcgaac aggtgttggc ctacgcgatc cccccctcac cagggttgca caaactgtgt
      901 gtccgagtga agggttcgga caatagctgg gggcctgtgt tcagcacgat catggacgtg
      961 gtcgcaatgc gcgacgctcg agtcattact gcagagtgct tttgggatgc ggatccaggg
     1021 ccggggagcg gtacaccgat gcttgcgttc gacggcaact tcaatagcgc ttttgaacag
     1081 ttgagcggga acctgaccgc accctccgct gggctgcacc gcctgagtat ccgcgtgcgc
     1141 ggagtggaca atacctgggg gccggtcttc agcacgatag tggatgtggt gaacgcccgg
     1201 caagtgagga tcaccagtgc cgagtatttc tgggacaccg atcccggagc cggtaacgga
     1261 cagccgatga tcgccttcga tggtgcgttc aacagtgtgt tggaacagat gtccgccacg
     1321 gtgagcttga ccgccttgtc ggtgggaccg cacgtgctcc acctgcgcgc aaagggtgcg
     1381 gacaatagct gggggccggt ccaccggtcc gttgtccatg tggatccaca acccgaggtc
     1441 acggtatcgt tcgatctgaa agtagcgctg caaggatgca tgggaaccgc agcgctgatg
     1501 aacaacggcc tgcggaacgc gggactggtg ccactcgctg aaccgtacag cgcactggga
     1561 cacgacctgg gcctgagcgc cggtgccacc acgacgtccg cggtcctgag cgtgatgttc
     1621 ccgccaggag cttcggtagt ggattggatc ctggtcgagt tccatccggc actggccccc
     1681 tcgcagatca cagcggccat gcctttgctg cttaggcgcg ggggcaccgt ggcgcagacc
     1741 gatggtggat accccttcct catcacattg ccggcggcat cctactacgt ggtggtacgc
     1801 caccgcaacc acctgcctgt ggccaccgag agtcccgtgg ccatctccac gaacggcgcc
     1861 acggtggcca ttgatttcac cgccgcggcc gttcaggcct tt
//
LOCUS       DAOQOC010000631         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42032_length_1901_cov_3.967497, whole genome shotgun sequence.
ACCESSION   DAOQOC010000631 DAOQOC010000000
VERSION     DAOQOC010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42032_length_1901_cov_3.967497"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..704)
                     /locus_tag="PL106_10120"
     CDS             complement(192..704)
                     /locus_tag="PL106_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33464.1"
                     /translation="MERLLFSMDPAIRASLSLLEPLGNAEICGLWNSDHMAGRGIPSL
                     LTAAALSIAPTLGLHSVVSLSASYSTKYLANCGFEFMTNLGREGSFEFPIPGIHSNAM
                     LNTDLCGLIKARPDQRQRILSLRTAPAQWRTESPKGRSLEVEYALMEAIDADQPIYRW
                     LAEERLRQSA"
     gene            complement(941..>1901)
                     /locus_tag="PL106_10125"
     CDS             complement(941..>1901)
                     /locus_tag="PL106_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNA33465.1"
                     /translation="YSAIDGGGLVPVIGLGACLENIRIAASSIGVGLNISTLPLPDAQ
                     ELIAVISKDGSIKNIVPDPLAQWIPQRCTNRKKPIKRAIDDSVLRAMTDAVAEVTRCE
                     MHTVVDAEARAAIAEAIASAELVRVLSPIGHDEFFGHEVRWTTQEAEATRDGIDLATM
                     ELKPSARVAFKVASDPATMDLLRLWNGGSGFKYATRGSVTDSPALCLISTDRNDPGAM
                     LDAGRAMERMWLAATAHNLAVHPVSAPILLAHNVRFGGGKGMNPAERDAVIRTFEEVR
                     TRFKVGDREPMFLLRLCHAPPPTARSLRRSLEEVLHFYQVMHA"
BASE COUNT          395 a          577 c          559 g          370 t
ORIGIN      
        1 cggcagcggg atcgaccagg atctccgcat ttttgccaac cgccacaaaa ccgtccactt
       61 gggtgatcgg gaccacgttc gtggtggcgt gaacgatcga acttgcgccg agcgctccga
      121 gagaaaggat catacgtgac cacatgcgca cgcttttacg cgagcgatgg cttacacgtt
      181 accagatcgc ctcaagcact ctgacgcaac cgctcttccg ccaaccagcg ataaatgggc
      241 tggtccgcat caatagcttc catcagggcg tattccacct ccaacgatcg tccctttggc
      301 gattcggttc tccactgggc cggtgcggtc cgtaaactca ggatacgttg acgttgatca
      361 ggacgtgctt tgatcaagcc acagaggtct gtgttcagca tcgcgttcga atggatgccc
      421 ggtattggga attcaaatga accttcccga ccgagattgg tcataaactc aaaaccgcag
      481 ttcgcaaggt acttcgtgga ataactggcg gaaagactga cgacggaatg aagtcccagc
      541 gtgggggcga tggaaagtgc agcagcggtc agcaacgaag ggatacctcg gccggccatg
      601 tgatcggagt tccaaagacc acagatctcg gcgttgccaa gtggttccaa caagctcagg
      661 ctggcgcgga tcgccgggtc catgctgaac aataaacgtt ccatggccaa tggtgatcca
      721 ggtcgcgcga tgtcaaggcg gacgccacca accactccga agtaggcatg ctcggcaacc
      781 accaccaccg atcggggatc cgtgcaccag gaataatcca tgcccaaggc gagatacacc
      841 ccgatattct caagtacgcg ggcatggccc acaacatatc ggacggtgct ctcccgatcg
      901 atcgccgatc ggaatgcccg gatccgtatc ctctcagtgg tcatgcgtgc atgacctgat
      961 agaagtgcag gacttcctcc agtgacctgc gcaaactcct ggccgtgggt ggtggagcat
     1021 ggcaaagccg caagaggaac atgggctcgc gatcaccaac cttgaagcgt gtgcggactt
     1081 cttcgaacgt ccggatcacg gcatcgcgtt ctgcggggtt catgcctttg ccacccccga
     1141 accggacatt gtgcgcgaga aggatcgggg cggataccgg gtgtacggcc aggttgtgcg
     1201 cggtagcggc cagccacatg cgctccatgg cgcgcccggc atccagcatt gccccggggt
     1261 cattccggtc ggtgctgatc aagcacaggg caggggagtc cgtgaccgat ccgcgcgtag
     1321 cgtacttgaa gccgcttccg ccattccaca accgcagcag atccatggtg gccgggtcgg
     1381 atgcgacctt gaaagccaca cgagcactcg gcttcaactc catggtggcc aggtcgatcc
     1441 catcgcgtgt ggcctccgcc tcctgcgtcg tccatcgcac ttcatggccg aagaactcat
     1501 cgtggccgat gggactcagg acacggacca gttctgcgga tgcgatcgct tcggcgatcg
     1561 ccgcacgggc ctccgcatcc actaccgtgt gcatctcgca acgggtcacc tccgctacgg
     1621 catcggtcat cgcacgcagt acgctgtcgt cgatcgcccg cttgatcggt tttttccggt
     1681 tggtgcagcg ctgggggatc cactgtgcca gggggtcggg cacgatgttc ttgatgcttc
     1741 cgtccttgct gatgacagcg atcaattcct gtgcatcggg aaggggcaac gttgagatgt
     1801 tcagcccgac gccgatggaa cttgcggcta tccggatgtt ctccaggcag gcaccaaggc
     1861 cgatgaccgg tacgagcccg ccaccatcga tcgccgagta g
//
LOCUS       DAOQOC010000632         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42034_length_1901_cov_3.775190, whole genome shotgun sequence.
ACCESSION   DAOQOC010000632 DAOQOC010000000
VERSION     DAOQOC010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42034_length_1901_cov_3.775190"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..1281
                     /locus_tag="PL106_10130"
     CDS             424..1281
                     /locus_tag="PL106_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNA33466.1"
                     /translation="MVSLLLSLPVFLLVYPHLPRWVLPVGHGWRVDHLLALVLIMAAF
                     IVLVRRFQLAVYIMLVAGLGTITVTSLTGRYGFRDLFQDYTVLLSSMRGNTEPLPMLL
                     QEEGPFPGAAEISAHIDYRDPVVRSFAVRAATTWFTDLDVPEEDAVLVQCFSVFKVIN
                     SNWRYVNDAQGVEYFAKASESTALLAGDCDDHAILMAACIKAIGGNVRLVRTTGHIYP
                     ELRVGNAADMERAATLIRERLFPRSARHATLFYHIDPKGDRWINLDYTRDYPGGEVMD
                     EAIRGVMGV"
     gene            complement(1358..>1901)
                     /locus_tag="PL106_10135"
     CDS             complement(1358..>1901)
                     /locus_tag="PL106_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin protein"
                     /protein_id="HNA33467.1"
                     /translation="VDWVLVELRNAATPATLVRTQAALLQSDGDIVGLDNNSTLTLPV
                     PAGTYHVAIRHRNHLGVMTGTAVALSSSPTSLDLTTGLVGTYGTNALKTVGAYRVLRT
                     GNSFRNTNIKYTGSQNDRDPILIRVGSITPNNTANGYFQEDVNLNGQVKYTGSANDRD
                     PILLNVGSIAPNNTVTEQLP"
BASE COUNT          348 a          537 c          612 g          404 t
ORIGIN      
        1 accggagtag gggttcgccg tttccggccc accaatggat gaactatccg cctggacctg
       61 ggcttgcgcc gatccgtatg tcagggcgca ggcgagtagg atatgccacc ggttttttgc
      121 caacggcggc gaatttaccc cgataccccg aactggaacc gcgccagggc gccttccgcc
      181 aacagagggg cgttggcaat ggatgtatcg gtccatatcc acgatggaac gacggatcgc
      241 gccgtcgaac atatttgtga aatgatgcga atggccgatg gtaacggagg cgcccgggtt
      301 gatcgttgtg atccattgtt caatttactt ggcactgtgg tacaagagcg gggcaacggg
      361 agcaggaagg agggaggcgg ccggagcagt tggccggatg cagcgcatgg ggttatcatc
      421 tggttggtat cgcttctgct gtccctgccg gtcttcctcc tggtgtatcc gcaccttcca
      481 cggtgggtgc tcccggtagg gcatggttgg cgggtggatc acctgctggc attggtactg
      541 atcatggccg cgttcatcgt gctggtacgc cggttccaac tggccgtata catcatgctg
      601 gtggcgggcc tcggcacgat cacggtcacc agcctaacag ggcgatatgg attccgtgac
      661 ctcttccagg actacacggt cctcctatcg tcgatgcggg gcaacaccga acccttgccc
      721 atgttgttgc aggaggaagg gcctttcccg ggcgcagcgg agatcagcgc tcatattgac
      781 tacagggatc ccgtggtgcg ttccttcgct gtgcgtgcgg ccaccacctg gttcaccgat
      841 ctggacgtgc ccgaagagga tgccgtgctc gtacaatgct tctccgtgtt caaggtgatc
      901 aattccaatt ggcggtacgt gaatgatgcg cagggagttg aatatttcgc caaggcgagc
      961 gagagtacgg ccctgttggc cggcgattgc gatgatcacg ccatcctgat ggccgcatgc
     1021 atcaaagcca ttggcgggaa cgtccgattg gtgcggacaa cgggacatat ctaccccgag
     1081 ttgcgcgttg gcaacgcggc cgacatggag cgtgcggcca cactcatccg ggagcgactg
     1141 ttcccgcgat cggcccgcca cgccacgttg ttctaccaca tcgatcccaa gggggaccgt
     1201 tggatcaatc tggactatac gcgcgactat ccaggaggcg aagtgatgga tgaggccatt
     1261 cgcggagtga tgggggtgtg atcggggagc agaaaagcag aaggccgtgc aacggaccgg
     1321 ccccggtcag tcgcacggcc ttcccatcac gttccggtca gggcagttgt tccgtcaccg
     1381 tattgttcgg cgcgatgctg ccaacgttca gcaggatggg atcacggtcg ttcgcacttc
     1441 cggtatattt cacctgcccg ttcaggttca cgtcctcctg gaagtacccg ttcgccgtgt
     1501 tgttcggtgt gatgctgccc acccggatga gtatggggtc gcgatcgttc tggctgccag
     1561 tgtacttgat gttggtgttc cggaacgaat tcccggtccg gaggacgcgg tatgcaccga
     1621 cggtcttcaa cgcattggtg ccgtacgtcc cgaccagacc cgtggtgaga tcgagcgagg
     1681 tgggcgagga ggagagcgcc acagccgtcc ccgtcatgac acccaaatgg ttgcgatgac
     1741 ggatggccac atgataggtg ccggccggta cgggcagggt gagcgtgctg ttgttgtcca
     1801 atcccacgat gtccccgtcg ctctggagca aggctgcctg ggtgcgcacc aacgttgctg
     1861 gcgtggctgc gttacgcagt tccaccagca cccagtcaac c
//
LOCUS       DAOQOC010000633         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42083_length_1900_cov_3.750136, whole genome shotgun sequence.
ACCESSION   DAOQOC010000633 DAOQOC010000000
VERSION     DAOQOC010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42083_length_1900_cov_3.750136"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1900
                     /locus_tag="PL106_10140"
     CDS             <1..>1900
                     /locus_tag="PL106_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33468.1"
                     /translation="LMSILFRSLLTCFVLAATLPSLATHNEAGEILVCHLGGLEYEVT
                     IVTHTNPNSQADRPEFVIEWGDGELDTVPRLSDTILTFGSVQVKRNLYVATHTYAAAG
                     VYTLQYIDPNRVAGIMNIEGSVDIPMCVQSQIIVTAALNDCLPRFLNAPIQLACIYQP
                     WIHNPAAYDEDGDSLSYEPVICLGPDGDDPNSLGDPISNYLYPDQYMPGPGNQYSMDP
                     VTGTITWDSPPMQGWYNIAFIVREWRDGAMIGWVERDMLIIVVSPCANEPPELGEMID
                     TCVVAGTPLTFPVSATDPDFGQVITLQAFGGPLVVDQSPAVFTTNPGTSPVSGTFAWN
                     TQCAHVRQQPWQVTFSVYDNYNPSLYDFGAAYVTVVAPAPLNPSATPDGSIMHLAWDP
                     DACSNAIGYRIYRRQQLYGFSPDHCETGVPGYTGYTLISTTTGWGSTTYDDHDLSFGV
                     TYCYMVTAIFADLAESYASVEFCNMLERDVPILTHVSVGATDVIAGIDTVRWSNAWDL
                     DTVQHPGPYHFKLFVGDGFTSASTLLYTSGPSPFLQHPDTSYLHTGINTVNGAHAYRV
                     ELYGANDTFIGSGNTASSVFLVPEPNDEAVTLHAQHSTPWINTLYEYYRETSPDVFTL
                     VGTGTTGTY"
BASE COUNT          373 a          620 c          539 g          368 t
ORIGIN      
        1 gctcatgagc atcctcttcc gctccctcct cacctgcttt gtgctggcgg ccacgctgcc
       61 ctccttggcc acgcacaacg aagcgggcga gatcctggtt tgccacctcg ggggcctgga
      121 atacgaggtc accatcgtta cgcatacgaa cccgaactcc caggccgacc ggccggaatt
      181 cgtgatcgag tggggcgacg gggagttgga taccgtgccc cgcctgtccg atacgatcct
      241 caccttcggc tcggtgcagg tgaagcggaa cctctatgtg gccacacaca cctacgctgc
      301 ggccggggtg tacaccctgc agtacatcga tccgaaccgt gtggcgggca tcatgaacat
      361 tgagggatcg gtggacatcc cgatgtgcgt gcagtcgcag atcatcgtaa cggctgccct
      421 gaacgactgc ctgccgcgct tcctgaacgc accgatccag ttggcatgca tttaccagcc
      481 ctggatccac aatccggcgg cctacgatga ggatggtgac agtctttcgt atgagccggt
      541 gatctgcctc gggccggatg gtgatgaccc gaacagcctt ggggatccca tcagcaacta
      601 cctctaccct gaccagtaca tgccggggcc gggcaatcag tactcgatgg atccggtcac
      661 gggcaccatc acgtgggatt caccccccat gcaaggctgg tacaatatcg ccttcatcgt
      721 gcgcgagtgg cgtgatgggg cgatgatcgg ctgggtggag cgcgatatgc tcatcatcgt
      781 ggtgtccccg tgcgcgaatg aaccaccgga attgggtgaa atgatcgata cctgtgtggt
      841 cgctggaaca ccgctcacct tcccggtctc cgccaccgat ccggacttcg gacaggtgat
      901 cacccttcaa gcattcggag gacccttggt ggtcgaccag agccctgcgg tcttcaccac
      961 caatccgggc accagccctg tttcgggcac gttcgcatgg aatacgcagt gcgcccatgt
     1021 ccgccagcaa ccctggcagg tcacgttcag cgtatacgac aactacaacc cgtcgctcta
     1081 tgatttcggg gccgcgtatg tgaccgtggt agcgccggcc ccactgaacc cttcggccac
     1141 accggacggc agcatcatgc atctggcctg ggacccggac gcgtgttcca acgccattgg
     1201 ataccgcatt tacaggagac aacagttgta cgggttcagc cccgaccact gcgagaccgg
     1261 tgtgcccggc tacaccggtt acaccctcat cagcaccacc acgggctggg gaagcaccac
     1321 gtacgatgac catgacctgt ccttcggggt aacctactgt tacatggtga ccgccatttt
     1381 cgcggacctc gccgagagtt acgccagcgt ggagttctgc aatatgctcg aacgggacgt
     1441 gcccatcctg acacatgtga gcgtgggcgc cacggatgtg atcgccggta tcgataccgt
     1501 tcgttggagc aacgcctggg acctggacac cgtgcagcat ccgggcccgt accacttcaa
     1561 gttgttcgtg ggcgatggtt tcacatcggc gagcaccctg ctgtacacga gcggcccttc
     1621 acccttcctc cagcatccgg acaccagcta cctgcatacg ggcatcaaca cggtgaacgg
     1681 agcacatgcc taccgcgttg aattgtacgg tgcgaacgac actttcatcg gcagtggcaa
     1741 caccgcctcc tcggtcttcc tggtgccgga accgaacgat gaagcggtga ccctccacgc
     1801 gcagcacagc acaccttgga tcaacacgct gtacgaatac taccgggaga cctccccgga
     1861 cgtgttcacc ctcgtgggca ccggtaccac gggcacgtac
//
LOCUS       DAOQOC010000634         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42144_length_1899_cov_3.219089, whole genome shotgun sequence.
ACCESSION   DAOQOC010000634 DAOQOC010000000
VERSION     DAOQOC010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42144_length_1899_cov_3.219089"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PL106_10145"
     CDS             complement(<1..271)
                     /locus_tag="PL106_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNA33469.1"
                     /translation="MIHRNFHIGLALLALVIPCVLPAQKIAFVDSKYILEQMPDYQAA
                     QQELDHHSAQWQKEIDDRWSQIKRMRDAYNAEAILLTDEMKTSRMD"
     gene            complement(336..>1899)
                     /locus_tag="PL106_10150"
     CDS             complement(336..>1899)
                     /locus_tag="PL106_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNA33470.1"
                     /translation="NPSGVDISSLYMDRGYLSFYLDPVERVEGDSIDLEVRIREGRQY
                     RVREVTISGNSKTFDHVVRREIRTLPGDLFDRSEVIRSQQQIVGLGFFDPEKVGVNPT
                     PDPRTGLVDLNYTVEEKPSDRLELSGGYGAGRLLLSLGLSFTNFSLRKITDPKAWSPL
                     PAGDGQTLNLRAQTNGKYYQSYSLSFVEPWLGGRKPNSLSLSVYYTRQTNGRAPGDLT
                     AAGTNAFAELGIFGSSLGFGWRLKWPDDYFFFNLTASFQRYSLNNWATSAGSSALFSF
                     ADGTSDVYAIQARFARRSIQEPFWITQGSETSFSLKATPPFSAFGANRNINYVALEPA
                     QRYKLAEFHKWKFTTQWFTKLTRSKTRHDLVFMARAGFGFLGRYNSRKGDSPFERFYL
                     GGSGLTGFQLDGREIIALRGYDDLSLTPTTGSFFVNKYTAELRFPVSLNPSATIFATT
                     FVEGGNAWNDIKRYDPFRLYRSAGIGLRLFLPMFGPMGLDYAWRFDDVPGAPSMAKSQ
                     FHFTIGIDLGEL"
BASE COUNT          343 a          548 c          646 g          362 t
ORIGIN      
        1 cgtccatgcg cgaggtcttc atctcatcgg taagcaggat ggcctccgcg ttgtacgcgt
       61 cgcgcatgcg tttgatctgc gaccagcggt catcgatctc cttctgccat tgggcgctgt
      121 ggtggtccag ttcctgttgg gcggcctggt agtcgggcat ctgctccagg atgtacttgc
      181 tgtccacgaa cgcgatcttc tgggccggaa gcacgcaggg gatcacgagc gccagaagcg
      241 ccagaccgat gtggaagttc ctgtgtatca tggtgatgac tggcctatgc aggggcgctt
      301 ggcccgaagg tccgcgaaag taggtgatca tcggctcaaa gctccccaag atcaatgccg
      361 atcgtgaaat ggaactggct cttggccatg ctgggcgcac cgggcacgtc gtcgaatcgc
      421 caggcgtaat caagtcccat ggggccgaac atgggcagga acaggcgcag gccgatacct
      481 gcacttcggt acagccggaa gggatcgtac cgcttgatgt cgttccaagc gttgccgccc
      541 tccacgaagg tggtggcgaa gatggtggcc gacgggttca acgacaccgg gaagcgcagc
      601 tcggccgtgt acttgttcac gaagaagctg cccgtggtgg gcgtgagcga gaggtcgtcg
      661 tacccgcgca gggcgatgat ctcgcgtcca tcgagttgga agcccgtgag gccgctgccc
      721 ccgaggtaga agcgttcgaa aggggaatcg cctttgcgcg aattgtacct gccgaggaag
      781 ccgaaaccgg cgcgggccat gaacaccagg tcgtggcggg tcttgctgcg cgtgagcttg
      841 gtgaaccact gggtggtgaa tttccacttg tggaactcgg ccagcttgta acgttgggcc
      901 ggttccagcg ccacgtaatt gatgttcctg ttggcaccga aagccgagaa gggcggtgtg
      961 gccttcaaac tgaaactggt ctcggacccc tgggtgatcc agaagggctc ctggatgctc
     1021 cgccgggcga agcgggcctg aatggcgtac acgtcgctgg tgccgtcggc aaaggagaag
     1081 agcgccgatg agcccgcact ggtggcccaa ttgttcaggc tgtaccgctg gaaactggcc
     1141 gtgaggttga agaagaaata gtcgtcgggc cacttcaggc gccagccgaa gccgagggag
     1201 gaaccgaaga tgccgagttc ggcgaaggca ttggtaccgg ccgcggtgag gtcgcccgga
     1261 gcacggccgt tggtctggcg ggtgtagtac acggacaggc tcagcgagtt gggcttgcgg
     1321 cccccgagcc agggctccac gaaggagagg ctgtagctct ggtagtattt gccgttggtc
     1381 tgcgcgcgca ggttcagggt ctgcccgtca ccggccggca ggggactcca cgccttgggg
     1441 tcggtgatct tgcgcaggct gaagttggtg aaggacaggc caagcgagag cagcaggcgg
     1501 cccgcaccgt aacctccact gagttccaag cggtcgctgg gcttctcctc cacggtgtag
     1561 ttcaggtcca ccaggcctgt gcgcgggtcg ggcgtgggat tcactcccac tttctcgggg
     1621 tcgaagaagc ccaggccgac gatctgctgc tgggagcgga tgacctcgct gcggtcgaac
     1681 aggtcgcccg gcagtgtcct gatctcccgg cgtaccacgt ggtcgaaggt cttggaattg
     1741 ccactgatcg tcacctcgcg cacgcggtac tggcgcccct cccgtatgcg cacctccagg
     1801 tcgatgctgt cgccctccac gcgctccacc gggtccagat agaaactcaa gtagccccgg
     1861 tccatgtaca gcgagctgat gtcaaccccg ctggggttc
//
LOCUS       DAOQOC010000635         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42354_length_1894_cov_4.892877, whole genome shotgun sequence.
ACCESSION   DAOQOC010000635 DAOQOC010000000
VERSION     DAOQOC010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42354_length_1894_cov_4.892877"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1663)
                     /locus_tag="PL106_10155"
     CDS             complement(80..1663)
                     /locus_tag="PL106_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33471.1"
                     /translation="MLTQSYGQDHFQRSFGPMTDTGAFDVVDAVRHPNGSWTMRTNDE
                     GARTILGLEPDGTNDWITRLTSSQFNSNGGAQRIADAGVNGSIVVLSDTMLSMSPTVS
                     RRHFDLIGLGATGNLLWSKRHYFDIPSTLLFPSTPHHVAPTTTGGIYVEGGEPELPAI
                     FKFSSTGVTEWGRSIDGPSPIGNIRDMIPDGNGGCYFLADGLRISDDSSRFVIGRLNA
                     NGALAWCKTARPAQSGRVIEPKNVILRANGDLLLLGLEYGATETWRGILLEMEADGDP
                     VWIMKYAYAGGGSMDLYSVRERSDGRLWMDFQSGGYGFILLNIDGTIDHADRFTYQQV
                     GSNVGVVAWSQTLMHNDELGVVGAYFIDDQGQPGPRTEWLWNLDAMEPDLCGTDPVVV
                     EAYQAQLNDLVFEQGFSVSNLNVGEAPLTVQASAVDLPPSLSSCSIILDVTEPEVEPA
                     MSIVPSIVAEGMQFVVRADGLSTVQISDLSGRKVMFTSSGSNNRVSVHTNGWGSGIHM
                     VCARDERGAIIGIGRVMVQ"
BASE COUNT          385 a          571 c          552 g          386 t
ORIGIN      
        1 gtcgtgatga gaagtgcgtg ccgcatcgtg ccgaaagtat ggtgcaggtg acttgcaggg
       61 cgcaaagcag gtactccgtt cactggacca tcacccggcc gatcccgatg atcgcgccac
      121 gctcgtcacg agcgcacacc atgtggatgc cagatcccca gccgttggtg tgtaccgaaa
      181 cccggttgtt gctcccacta cttgtgaaca tgaccttgcg gccgctcaga tcggagatct
      241 ggacggtgct gaggccatcc gctcgcacca cgaactgcat gccttcagcc actatggagg
      301 gtacgatcga catggcgggc tcaacctccg gttccgtcac gtcgaggatg atggagcacg
      361 aggacaacga gggcgggagg tcaacagccg aggcttgcac agtgagcggg gcttcaccca
      421 cgttcaggtt ggagacggaa aaaccctgtt cgaacaccag gtcgttcagt tgcgcttggt
      481 aggcttcgac caccaccgga tcggtgccac agagatccgg ttccatcgca tccaggttcc
      541 aaagccactc cgtacgcggc ccgggctggc cttggtcatc aatgaagtag gcgcccacaa
      601 ctccgagttc atcattgtgc atcagggtct ggctccaagc gaccacccca acattgcttc
      661 cgacctgttg atacgtgaag cgatcggcgt gatcgatcgt cccgtcgatg ttgagcagga
      721 tgaagccgta tcctcccgat tggaagtcca tccacaggcg gccatcggaa cgctcacgca
      781 cgctgtacag atccatggat ccgcctcctg catacgcgta tttcatgatc cataccggat
      841 ccccgtcggc ctccatttcc aagagaatgc cccgccatgt ttcggtcgca ccgtactcaa
      901 gcccgagcag gaggaggtcg ccgttcgcgc ggagaatgac attcttcggc tcgatcacgc
      961 ggccgctttg cgcaggcctg gcggtcttgc accatgcaag ggcgccgttc gcgttcagtc
     1021 gcccgatgac gaaccggctg ctgtcgtcgc tgatccgcag gccatctgcc aaaaaatagc
     1081 agccgccatt gccatccggg atcatgtccc tgatgttacc gatcgggctc gggccgtcga
     1141 tggaccggcc ccactcggtg accccggtac tgctgaactt gaatatggcg ggcagttccg
     1201 gctcacctcc ttccacatag attcctcctg ttgtggtagg ggccacatgg tggggggtac
     1261 tcgggaacag gagagtggat gggatgtcga aatagtgacg cttggaccaa agaaggtttc
     1321 ccgtggcgcc gagcccgatc aggtcgaagt gacgtcgact caccgtcggg gacatgctga
     1381 gcatcgtgtc actgagtacc acgatcgaac cgttcacacc cgcatcagca attctttgcg
     1441 ctccgccgtt gctattgaat tgcgaagaag tgagccgggt gatccaatca tttgttccgt
     1501 caggctccaa cccgagtatc gtccgtgcgc cttcgtcatt cgtacgcatc gtccatgagc
     1561 cgttcgggtg tcgtaccgca tccaccacat cgaaggcgcc cgtatccgtc atagggccga
     1621 acgagcgttg gaagtgatcc tgaccataag attgggtcag catcgccgaa agtgcggtga
     1681 tcaggacggg tcgatgggga gacatgatgc caatatatcc gcttgacggt ttccaaagcg
     1741 ttggacgatg agggctacct tcgcagccct gtatgccagt gcgatcaagc caagggccta
     1801 cccgtatgac caccgtccaa ctacacgatc tgcggttcga acccttcatc tccgaacagg
     1861 aactgggccg ggccatcgat gtggtggcgg tggg
//
LOCUS       DAOQOC010000636         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42387_length_1894_cov_2.355084, whole genome shotgun sequence.
ACCESSION   DAOQOC010000636 DAOQOC010000000
VERSION     DAOQOC010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42387_length_1894_cov_2.355084"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..612
                     /locus_tag="PL106_10160"
     CDS             187..612
                     /locus_tag="PL106_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33472.1"
                     /translation="MKAPNSLLRRMLPMTAVLLLFASFTPPASAPQLQIGTDLNCNFT
                     VRIHATGGSCGAATEPSAVFCVQISGPTTVQFNLPGGTTVVQRIELYCGLHNCGTTAP
                     LAPVIAVWDCLNGTTTGTCCDEVVGIYGDQPTGGWRIEP"
     gene            619..>1894
                     /locus_tag="PL106_10165"
     CDS             619..>1894
                     /locus_tag="PL106_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33473.1"
                     /translation="MRTTQLTARYRVLLVLLFSWATLPFCAHAQLRNANWLYVNNNFI
                     SFASGTAIASTAPVGGVNSNASLSDPEGNPLVYAFGNTVVNAAFETVSDCPILNMSAE
                     GQGCLLMPVPGDAHGVAFFHIAYGNGMTASPLSFGRINMDLDNGLGGFVGGMTLLTDS
                     ATWKLTAVAHTNGQDYWVLTQLFGTDAIAAYLLDGTGVSAVPVVSHTGDPMTYPLNGT
                     PYEYLPGSLVATSDGRRLAMAGVLPQNVEVFNLEHARIDLLDFDPATGIATHAASIPP
                     PAGLTIDGIEFSPDGSKLYIAITGNGNVSNFCAIHQYDLSVWDEASIIASRTEVLLDT
                     AINFYWGPELQLDLAPDGRIWFHNNYDHWLGVIDSPDEAGPDCAPIRDYLLLPNEGGY
                     GLPNQCKRYHDSELTHTGIPASEEAGTLMTWPN"
BASE COUNT          382 a          598 c          531 g          383 t
ORIGIN      
        1 cgcagcgccc ggcgatgatg gaggaggatc attgagaaag gttgagaggg tgagagacct
       61 gctctcgcac gctcgcactc tcgcacggat gatcgaccgg tcctgttgcc gctccaaatc
      121 agatatcaac ccgcgtatcc gaacgcggca tgaaccatac cttgcgatcc agcaaaacga
      181 acaaccatga aagcccccaa ctcccttcta cggcgcatgc tgccgatgac cgcggttttg
      241 ttgctctttg ccagctttac tccacccgcc tccgcaccac agctacagat cggaacggac
      301 ctgaattgta acttcactgt gcgcatacac gcaacaggag gaagctgcgg ggcagcgacg
      361 gaaccgtccg cggtcttttg cgtgcaaata agcggaccca ccacggtaca attcaacctg
      421 cccggtggca ccacggtggt acagcgcatt gaactttact gtggactcca caactgcggc
      481 accacggcgc cacttgcccc tgttatcgcc gtttgggact gcttgaacgg aacgaccaca
      541 ggcacgtgtt gcgacgaagt ggtcggtatc tatggtgatc agccaacggg tggttggaga
      601 attgaaccct gagctgctat gagaaccacg cagctcacag cccggtacag ggttcttctt
      661 gttctgctgt tcagttgggc gacccttccc ttctgcgccc atgcccagtt gcgcaatgcg
      721 aactggcttt acgtcaacaa caatttcatt tcgttcgcca gcggcaccgc catagcctcg
      781 accgcccctg tcggcggggt caattccaat gcgagcttga gcgacccgga gggcaacccg
      841 ctggtatacg cattcggtaa cacggtggtg aacgcggctt ttgagaccgt gagcgactgc
      901 ccgatcttga acatgagcgc ggagggtcaa ggctgcctgt tgatgcccgt tcctggtgat
      961 gcccatggcg ttgcattctt tcacattgcc tacggcaacg gcatgacggc atcgcccctt
     1021 tccttcggcc gtatcaacat ggacctggac aacggattgg gcggctttgt gggcggcatg
     1081 acgctgctga cggacagcgc cacgtggaag ctcaccgccg tggcgcacac caacgggcag
     1141 gattattggg tactcacgca actgttcggc accgatgcga tcgccgccta cctgctggac
     1201 gggaccggtg tatccgccgt gccggtggtc agccacacgg gcgatcccat gacctatccg
     1261 ctcaatggca ccccgtatga atacctgccc ggttcgctgg tggccacctc ggatggccgc
     1321 cgcctggcca tggcgggggt gcttcctcaa aacgttgagg tcttcaacct ggaacacgcg
     1381 cgcatcgacc tgctcgactt cgaccccgcg acggggatcg ccacacacgc ggcatccatt
     1441 ccgccacctg caggtcttac catcgatggc atcgaattct cgccggacgg gtcgaagctc
     1501 tacatcgcga tcacggggaa cgggaacgtc tcgaatttct gtgcgatcca ccaatacgat
     1561 ctttccgtgt gggacgaggc ctccatcatc gcctcgcgca cggaggtcct cctcgacacc
     1621 gccatcaact tctattgggg tcctgaactg caattggacc ttgctcctga tggtcgcatc
     1681 tggttccaca acaattatga ccattggttg ggcgtgatcg actcgccgga cgaagccggt
     1741 ccggattgtg cacccatcag ggactatctg ctgctgccga acgaaggcgg ctatggtctg
     1801 cccaaccagt gcaaacgcta ccacgacagc gagctgacgc ataccggtat tccggcatcg
     1861 gaagaggcgg gcacgctgat gacctggccc aacc
//
LOCUS       DAOQOC010000637         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42415_length_1893_cov_3.571273, whole genome shotgun sequence.
ACCESSION   DAOQOC010000637 DAOQOC010000000
VERSION     DAOQOC010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42415_length_1893_cov_3.571273"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PL106_10170"
     CDS             <1..321
                     /locus_tag="PL106_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNA33474.1"
                     /translation="NYCLDTLRKNQRHREEDIDERTQEGLPTDTTSEAALLGLRAERL
                     DHVLEQLDAMDRGVLLMKYEDELPVTDMMATLGIGESAVKMRLMRARERAIAKYHELY
                     PDEP"
     gene            318..518
                     /locus_tag="PL106_10175"
     CDS             318..518
                     /locus_tag="PL106_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33475.1"
                     /translation="MSDNPFKIIRPTDQPPESLKKDVMGSVKFVMLILRFVQLFVADL
                     PVSAFDKVRLIDPETGAPKKKN"
     gene            534..1385
                     /locus_tag="PL106_10180"
     CDS             534..1385
                     /locus_tag="PL106_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNA33476.1"
                     /translation="MDLTGLKDTLLRSFETYLGGIIAQLPSIASGLIVLLLGWLVAKL
                     LRAIVKNVTQKAGVDKVAERTGLSQVLAKVGVKKVSVLLAGLAYGLAMLIFIVAAAEV
                     MHMEGLSNAIDRFFGYLPTLATALAIFIGGLWGADKVKSQVSTVMESVGLGGAKMVAG
                     LLGGCIVLFTTITALNVAGVDTTLITSNILVVMAGILLAFGIAYGHAARDVLSNILGS
                     YYGKDRFKPGMRVRVGNDEGVIEKIDSISITIRTHDRLVLLPTRQLITERIEIIAEPE
                     EPGGGVA"
     gene            complement(1447..>1893)
                     /locus_tag="PL106_10185"
     CDS             complement(1447..>1893)
                     /locus_tag="PL106_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33477.1"
                     /translation="QAAGADEAGTASSNMKSITADNDGSQNAAVAKATPPPTPAIVTV
                     NQEQLSNEVAADAAVAEDHDRRANAMDSAITSSGATGHEVTEKELARNMSVAGVTEEQ
                     VRTKEEDMGKRGSRARTVSASKQKVDQGNAASTEASAYVGLLRAAW"
BASE COUNT          349 a          585 c          579 g          380 t
ORIGIN      
        1 aattactgtc tggataccct gcgcaagaac cagcggcacc gcgaggagga tatcgatgaa
       61 cgcacacagg agggcctgcc cacagatacg accagcgaag ccgccctgct gggcttgcgg
      121 gctgaacggc tggaccatgt gctcgaacaa ctggatgcca tggaccgcgg ggtcctcttg
      181 atgaagtacg aggacgaact gcccgtcacc gacatgatgg ccaccctcgg catcggcgag
      241 agtgcggtga agatgcgcct gatgcgcgca cgtgagcggg ccatcgccaa gtaccatgaa
      301 ctttatccgg acgaaccatg agcgacaacc cgttcaagat catccggccc actgatcagc
      361 cgcccgagtc cttgaagaag gatgtgatgg gcagcgtgaa gttcgtgatg ctcatcctgc
      421 gtttcgtaca gctgttcgtc gcggacctcc cggtcagcgc gttcgacaag gtccgcctca
      481 tcgatcccga aacgggcgca cccaaaaaga agaactgatc cacatcctcc cacatggacc
      541 ttacaggact gaaggacacc ctgctccgct cattcgagac ctacctcggt ggcatcatcg
      601 cccaattgcc ttccatcgcg agcggtctca tcgtgctgct cctcggctgg ctcgttgcca
      661 agctgttgcg cgccatcgtg aagaacgtaa cccaaaaagc gggggtggac aaggtggctg
      721 agcggaccgg cctgagccag gtcctggcca aggtgggcgt gaagaaggtg agcgtgctgc
      781 ttgcgggcct tgcctacgga ctggccatgc tcatcttcat cgttgccgcc gccgaggtga
      841 tgcacatgga aggcctgtcg aacgccattg accgcttttt cggatatctg cccacactgg
      901 ccacggcgct ggccatcttc atcggcgggc tctggggtgc ggacaaggtg aagagccagg
      961 tgagcaccgt gatggagagt gtggggctgg gtggtgccaa aatggtggca ggcctgctcg
     1021 gtggctgcat cgtcctgttc accaccatca cggcgctgaa cgtggccggt gtggatacca
     1081 cgctcatcac cagcaatatc cttgtcgtga tggccggtat cttattggcc tttggtatcg
     1141 cctacggtca tgcggcccgc gatgtgctct ccaacatcct cggtagttac tatggaaagg
     1201 accgcttcaa gcccggcatg cgggtgcggg tgggcaacga cgaaggcgtg atcgagaaga
     1261 tcgacagcat ctcgatcacc atccgaacgc acgaccgtct ggtgcttctg cccactcggc
     1321 aactcatcac cgagcgcatc gagatcattg cggagccgga agaaccgggg ggaggggtgg
     1381 cctgagcagg accgggaagg tccgtcggtg atcgcgggtc aaccggcacc cggcttccgg
     1441 tctccgttac caggccgcgc gtaacaggcc cacataggcg ctcgcctccg tggatgctgc
     1501 gttgccctga tccacctttt gcttggaggc cgataccgtt ctcgcccggg atccacgttt
     1561 gcccatgtcc tcctccttgg tacgcacctg ctcctcggtc acgcccgcta ccgacatgtt
     1621 gcgggccagt tctttctccg tgacctcatg gccggtagca ccggaactcg tgatggcact
     1681 gtccatggca ttcgcccggc ggtcgtgatc ctctgcaacg gccgcatcgg ctgccacctc
     1741 attgctcaac tgctcttggt tcaccgtaac gatggccggg gtcgggggag gagtggcttt
     1801 tgcgaccgcg gcgttctgcg atccgtcatt gtccgcagtg atgcttttca tgttggatga
     1861 ggccgtcccg gcttcgtccg caccggctgc ctg
//
LOCUS       DAOQOC010000638         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42452_length_1892_cov_4.074034, whole genome shotgun sequence.
ACCESSION   DAOQOC010000638 DAOQOC010000000
VERSION     DAOQOC010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42452_length_1892_cov_4.074034"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PL106_10190"
     CDS             <1..681
                     /locus_tag="PL106_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33478.1"
                     /translation="VWLAHKLEVQPLMENGEVATKPEYLVMLSSIGLLGTMTLLFMFA
                     HTRCPFFVWGQKRMGVHKAVAIPSASQRPLAMIAFIEMIMIVWTFYIVLLVCYDNDIA
                     GDRHPITWIVAFGSLAWSVYLMGKLLRIRSFDHAVRYAIPTVVIFWNFVEVMGRWNLF
                     KEIWVHPTEHWIENSLILATMVGFTAYYMYKGRKDRQRRVRPEIVDAGVPVRANPQGA
                     PRTASVIQ"
     gene            944..>1892
                     /locus_tag="PL106_10195"
     CDS             944..>1892
                     /locus_tag="PL106_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNA33479.1"
                     /translation="MEPLFPDDPSGELEAMAVELISRSAALSEALHPVTRAAVADLVR
                     PMNIYYSNLIEGHDTHPIDIDRALRNDYSTDKRKRDLQQEALAHIAVSKALRTGELRQ
                     ATGSDPSSEVFIMGVHRAFYEHLPADFLRVVSKEGVEQQVVPGVYRTGEVEVGRHIAP
                     GHEHLPHFMARFASFYDREAPGNRSRIHRVIAMAAAHHRLAWIHPFLDGNGRTVRLCT
                     DAWAMADDLDAGGVWSISRGLARKRDEYRAMLAGADAPRMGDLDGRGNLSNARLVTFC
                     RWFLATAIDQVSFMRDAFQLDGVLRRIDVFVDRMASEGRM"
BASE COUNT          353 a          577 c          579 g          383 t
ORIGIN      
        1 gtgtggctcg cgcataaact ggaggtacag ccgctgatgg agaacggcga ggtggccacc
       61 aagcccgaat acctggtgat gctcagttcc atcggcctgc tgggcaccat gaccctgctg
      121 ttcatgttcg cccacacccg ttgccccttc ttcgtgtggg gccaaaagcg catgggcgta
      181 cacaaggccg tggccatccc cagtgcctcg cagcgccccc tggccatgat cgccttcatc
      241 gagatgatca tgatcgtgtg gaccttctac atcgtactgc tggtgtgcta cgacaacgac
      301 atcgccgggg accggcaccc catcacctgg atcgtggcct tcggttcgct cgcttggagc
      361 gtctacctca tgggcaagct gttgcggatc cgttccttcg accatgcggt gcgctacgcc
      421 atccccacgg tggtgatctt ctggaacttc gtggaggtga tgggccgctg gaacctcttc
      481 aaggagatct gggtacaccc caccgagcac tggatcgaga acagcctgat cctggccacc
      541 atggtgggtt tcaccgccta ctacatgtac aagggacgga aggaccggca gcgtcgggtc
      601 cgtcccgaga tcgttgatgc aggggttcct gtgcgggcga acccgcaggg cgctccccgc
      661 accgcatccg tcatacagta ggtctgggct gttcggtcag ggttgagggc agcgttgagt
      721 gcgccaacgc accggtgttc ggaaatagca tcggaatcgt tatcggaaca ttgcatcctg
      781 atattaacta tattgctgtt tattagcatt ttatatcaaa ttcatccgtc ggaatattgt
      841 tgaccgaccc gctttcgaac ccctatcttt gacgttcgcc cggattgggc gtcagcgacc
      901 aggaatggct tcaccaatcc gcctttatac ggatcctgct gccatggaac cactgttccc
      961 ggatgacccg agcggggagt tggaagccat ggcggtggaa ctgatcagcc gatcggccgc
     1021 gctctccgag gcgctgcatc cggtgacccg cgccgccgtg gcggacctgg tacggcccat
     1081 gaacatctac tacagcaacc tgatcgaggg gcacgatacg cacccgatcg atattgaccg
     1141 cgcgttgcgg aacgactatt ccaccgacaa gcgcaagcgg gacctgcagc aagaagcact
     1201 tgcgcacatc gccgtaagca aggccttgcg caccggagaa ttgcggcagg cgaccggatc
     1261 ggatccatca tccgaggtat tcatcatggg cgtacatcgc gcgttctacg aacatctgcc
     1321 tgcggacttc ctgcgtgtgg tgagcaaaga gggcgttgag cagcaggtgg tgcccggcgt
     1381 ctaccggacc ggagaagtgg aggtgggtcg ccacatcgct cccggccatg aacacctacc
     1441 gcacttcatg gcgcgcttcg ccagcttcta cgaccgggag gcccctggga accgatcgcg
     1501 cattcaccgt gtgatcgcga tggcggccgc gcaccatcgg ctcgcatgga tccatccatt
     1561 cctcgacggg aatggccgta ccgtgcggct ttgcacggat gcatgggcca tggccgacga
     1621 cctggatgcc ggtggagtgt ggtccatttc gcgcgggctg gcgcgcaagc gcgatgaata
     1681 ccgggccatg ctcgcaggtg cggatgcccc gcgcatgggc gacctggatg gacgcgggaa
     1741 cctgagcaat gcccggctcg tgaccttttg ccggtggttc cttgccacgg ccatcgatca
     1801 ggtctcgttc atgcgcgacg cgttccaatt ggatggcgta ctgcgacgga tcgacgtctt
     1861 tgtagaccgc atggccagcg aagggcgcat gc
//
LOCUS       DAOQOC010000639         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42526_length_1890_cov_5.758038, whole genome shotgun sequence.
ACCESSION   DAOQOC010000639 DAOQOC010000000
VERSION     DAOQOC010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42526_length_1890_cov_5.758038"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1890)
                     /locus_tag="PL106_10200"
     CDS             complement(<1..>1890)
                     /locus_tag="PL106_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33480.1"
                     /translation="TAQLSFWQAYRDIGDAGDNTNVEVSTDGGFTWGPVIQYTSTQGA
                     ANAWVNAVLDLSAYAGQADVRIRFRYTATWDWGWAIDNVSVTGTGAALSYAWASTPAY
                     FTSGVQNPTGVTVSPLPTDFTVTASAPNGCSATATVTVILDPTDTDGDSQVDCVDACP
                     NDPNNDQDGDGICGDVDSCPTVFGQIGDPCDDGNCYTTGDVLNGSCVCAGTPVPCDTW
                     TLTIESDANGGEISWIIQEDGGPCVLQTGGPYANNSTNNVSVCVPTGNCFKLTMNDAG
                     GDGIVGGGWKLVDNNGRRILDNEDNGGCFGVTSTHGLAFCNEPASAQTVIGIHCDKTN
                     WLPTDVIIASEDPAVSAQWGIGDQTDDGYTFWFLDPCGSYNRRIFRNHATSGGHGPAN
                     ALRACKLKLSSMVSYPLPQNTLLNVRVRSRVNGVDGTWGPACRFMIDVNSCTLTQLNN
                     DISSPNYSCGVSGKVVGASGNTGKIFATVVTSGGNPATHYRFNFAVPGEGYSRNLVST
                     NAACLLGIWQTAPLLCGTYTYEVRVQASFDGGVTYCPYGTMCQVDITNTTAYCTAPGA
                     MAQQPETRELAESADGFAMYPNPNREGQLFLAMNGIADNVEVVSVDIYDMLGQRATSA
                     TIAVAG"
BASE COUNT          292 a          581 c          637 g          380 t
ORIGIN      
        1 cccgccacag cgatggtggc gctggtggca cgctgcccca gcatgtcgta gatgtccacc
       61 gataccactt ccacgttatc ggcaatgccg ttcatggcca ggaacaactg accctcgcgg
      121 ttggggttgg ggtacatggc gaagccatcc gcgctttcgg ccagttcgcg cgtttcaggc
      181 tgttgggcca tggcgccggg cgcggtgcag tacgcggtgg tgttggtgat gtccacctgg
      241 cacatcgtac cgtaggggca gtaggtcacc ccaccgtcga agctggcctg cacgcgcacc
      301 tcataggtgt atgtgccgca gagcagcggg gccgtctgcc agatgccgag caggcacgcc
      361 gcgttggtgc tcaccaggtt gcggctgtag ccctcgcccg gtaccgcgaa gttgaagcgg
      421 tagtgggtgg ccgggttgcc gccgctggtg accacggtag cgaagatctt gcccgtgttg
      481 ccgctggcgc ccaccacctt gccgctcacg ccgcagctgt agttggggct gctgatgtcg
      541 ttgttgagct gggtgagggt gcaggagttc acatcgatca tgaagcggca ggccggtccc
      601 caggtgccgt ccactccgtt cacacggctg cgcacgcgca cgttgagcag ggtgttctgg
      661 ggcagggggt agctcaccat gctgctgagt ttgagcttgc atgcgcgcag ggcattggcc
      721 ggaccgtggc caccgctggt ggcgtggttg cggaagatgc ggcggttgta gctgccgcag
      781 gggtccagga accagaaggt gtatccatcg tcggtctggt cgccgatgcc ccactgggcg
      841 ctcacggccg gatcctcgct ggcgatgatc acatcagtgg gcagccagtt ggtcttgtcg
      901 caatggatgc cgatcactgt ttgcgcgctg gccggctcgt tgcagaacgc caggccgtgg
      961 gtgctggtga cgccgaagca accaccgtta tcctcgttgt ccaggatgcg gcgaccgttg
     1021 ttgtccacga gcttccagcc gccacccacg atgccatcgc caccggcgtc gttcatggtg
     1081 agcttgaagc agttgcccgt gggcacgcac accgacacat tgttggtgct gttgttggcg
     1141 tagggaccac cggtctgcag cacgcagggg ccgccgtcct cctggatgat ccagctgatc
     1201 tcgccgccat tggcatcgct ctcgatggtg agggtccagg tgtcgcaggg cacaggtgtt
     1261 cccgcgcata cgcacgaacc gttgagtaca tcacccgtgg tgtagcagtt gccgtcgtcg
     1321 cacggatcac cgatctgacc gaagacggtg ggacagctgt ccacgtcgcc gcagatgccg
     1381 tcgccgtcct ggtcgttgtt cgggtcgttc gggcaggcat ccacacagtc cacttgggaa
     1441 tcaccatcgg tgtcggtggg gtccaggatc acggtcaccg ttgccgtggc actgcagccg
     1501 ttgggcgcac ttgcagtaac ggtgaagtcc gttggcaggg gcgataccgt aacaccggtg
     1561 gggttctgca cgccgctcgt gaaataggcc ggcgtggacg cccacgcgta gctgagtgcc
     1621 gcgccagtgc ccgtaacgct cacgttgtcg atggcccaac cccagtccca ggtggcggtg
     1681 tagcggaagc ggatccgcac atcggcctgg ccggcgtatg cgctcaggtc gagcactgcg
     1741 ttcacccacg cattggccgc gccttgcgtg ctggtgtatt ggatcacagg tccccaggtg
     1801 aatcctccgt ccgtactcac ctccacgttg gtgttgtcgc cggcatcgcc gatatcccgg
     1861 taggcctgcc agaagctcaa ttgagccgtg
//
LOCUS       DAOQOC010000640         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42533_length_1890_cov_3.786376, whole genome shotgun sequence.
ACCESSION   DAOQOC010000640 DAOQOC010000000
VERSION     DAOQOC010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42533_length_1890_cov_3.786376"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PL106_10205"
     CDS             <1..1070
                     /locus_tag="PL106_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33481.1"
                     /translation="DHLCDMFSDECELMIPARDFALSFTGGFDGRTLLAAALALGRKD
                     FTTYGFGSPGESDIVLPGMQARMLGLPYRSVLLDDKYVREDALKAALTFMELSGQEGN
                     LGRPHYLHAAELLSKQHPYLVTGNFGSELFRALHTPGEMMTGHLIDAFSPVDDAWRSR
                     LFAAAGPAFAHEAAEVVDTLAAYLAEVADRSPSRRFYRFVFDELLRKYFGPEIMVQSH
                     FLRNRTPFLSLRFVKAVHHTEWAGVHARLFEQDRSRRMRGQKFYAAFLRRASPRLYHM
                     RTNKGYMPVDLDRSWRLPLLVARSLWKRILVAEHPDSNAVGALLNLHRAAILERYGLI
                     DQDDDIGRLSARVAWAACGGK"
     gene            complement(1128..1676)
                     /locus_tag="PL106_10210"
     CDS             complement(1128..1676)
                     /locus_tag="PL106_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNA33482.1"
                     /translation="MAHIKLKNEELPGIIGLMHYRPETAEPLNQLAETLLRGPSTLTP
                     GEREIIASSVSWWNDCHFCHTSHGAAAAAHHGGDLNLIDEIKAGLPDRDLSPKLRALL
                     AIAKHVQQGGKKVTGTDIAAAREAGATDLEIHDTVLIAAAFCMYNRYVDGLGTWRPEP
                     KEAYKEVGEMLAFKGYLEAVPA"
BASE COUNT          312 a          560 c          588 g          430 t
ORIGIN      
        1 tggatcactt gtgcgacatg ttctcggacg agtgtgagct gatgatccca gcacgcgatt
       61 tcgcactttc cttcaccggt ggattcgatg gccgcacgct gctggcagct gcgctcgcac
      121 tgggtcgcaa ggacttcacc acctatggtt tcggatcccc gggtgaaagt gatatcgtcc
      181 tgcccgggat gcaagcccgc atgctggggt tgccgtaccg gtccgttcta cttgatgata
      241 agtacgtgcg cgaagatgcg ttgaaagcgg cactgacctt catggaactc agtggacagg
      301 aaggcaacct gggccgaccg cattatctcc atgcagcgga actgctctcc aagcagcatc
      361 cgtacctggt aacagggaac ttcggcagtg agctcttcag ggccttgcac acgccgggcg
      421 aaatgatgac cggacacctg atcgacgcgt tcagtccggt ggatgatgcc tggcgttcgc
      481 gactgttcgc ggcagcaggt cctgcgttcg cgcatgaggc cgcggaagtg gtcgatacgc
      541 ttgcggccta cctggctgaa gtggccgacc ggagcccctc ccggcgtttt taccgttttg
      601 tgttcgatga actcctgcgc aagtatttcg ggccggagat catggtgcag tcgcattttc
      661 tacggaaccg cacaccgttc ctgtccttgc gcttcgtgaa ggccgtccat cacaccgaat
      721 gggccggtgt gcatgcgcgg ctgttcgagc aggaccggtc gcggcggatg cgcggacaga
      781 aattctacgc ggccttcctt cgccgtgcct ccccgcgcct gtatcacatg cgcacgaaca
      841 agggatacat gccggtggat ctggaccggt cctggcgctt gccacttctg gtagcgcgtt
      901 ccttgtggaa gcgcatcctg gttgcggaac accctgattc gaacgcggtg ggagccctgt
      961 tgaacctgca cagggcggcc attcttgagc gttatggttt gatcgatcag gacgatgata
     1021 tcggccgctt gtcggcgcgg gtcgcctggg ccgcttgtgg cgggaaatga ataagaacgg
     1081 ccctgcggag agtagagggc cgttcgtcgc tcgctcgtgt gtcgtggtca ggccggtact
     1141 gcttccagat accctttgaa agcgagcatt tcacccacct ccttgtaggc ttctttgggc
     1201 tcgggacgcc aggtgcccag cccgtccacg tagcggttgt acatgcagaa cgctgccgcg
     1261 atgagcaccg tgtcatggat ctccaggtcc gtggcaccag cttcccgcgc agcggcgatg
     1321 tccgttcccg tcactttctt gccgccttgt tgcacgtgct tggcgatggc gagcaatgcg
     1381 cgcagcttcg ggctcaggtc ccgatcggga aggcctgcct tgatctcatc gatcaggttg
     1441 aggtcccctc cgtgatgcgc tgcagcggcc gcaccgtggc tcgtgtggca gaagtggcaa
     1501 tcgttccacc agctcacact gctggctatg atctcccgtt cgcccggtgt gagtgtgctc
     1561 ggcccgcgca gcagcgtttc ggccagttgg ttcaagggct cggcggtctc cggtcggtaa
     1621 tgcatcagtc cgatgatacc gggcagttcc tcgttcttca gcttgatatg cgccatggtg
     1681 gttcgaatga gttgttcacc aaaggtccat gctcacgcac cgccaacgcg gtgggtcctg
     1741 cggatcgggt gaaatgaatg gtgaacccgc gatccgtgtt tgtggatgcc cacgcgggct
     1801 ccttcggggt ccacctgtag ggatggtaac atccagccga ccatgtgcct gcgcttcctg
     1861 acctttgtcg ttcttccatg gatagccgcg
//
LOCUS       DAOQOC010000641         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42553_length_1890_cov_2.951499, whole genome shotgun sequence.
ACCESSION   DAOQOC010000641 DAOQOC010000000
VERSION     DAOQOC010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42553_length_1890_cov_2.951499"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..1117
                     /locus_tag="PL106_10215"
     CDS             518..1117
                     /locus_tag="PL106_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNA33483.1"
                     /translation="MSRLPVMESFYTLQGEGLFAGQAAYFIRLGGCDVGCAWCDVKES
                     WDASAHPTRAVNDIVSEASAYPARIAVITGGEPLMHDLSELTASLRSAGFRTHIETSG
                     THPFSGDWHHVCLSPKKFKAALPGAYPRADELKVIVLNRHDLHWAVEQAAGTRPDCVL
                     YMQPEWSKRESAMPLITEHIKAHPRWRVSLQTHKYMNIP"
     gene            1114..>1890
                     /locus_tag="PL106_10220"
     CDS             1114..>1890
                     /locus_tag="PL106_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33484.1"
                     /translation="MRFRLTPIFELAVGSRQQAAILVQVALLALCCLLPPGAFAQPGS
                     YTTTDKRAIKLYESGGDCMRQRKWACAQEDLEKAAKQDPSFIEPRILLAEMYEQQDKA
                     TDAMAAWNEVLAISPKYFKPAALHQAELQFTAGKYDDAEKNYRLYLTLDDDPLRKSRA
                     LLGIEKCAFARVAVQHPVPFEPKNLGPGVNTSDPEYYPCITADGRTLLFTRRVAEPSA
                     QYGMQEDFYISYKGDTAWNRASPVPSVNTTTMNEGAGTLSP"
BASE COUNT          388 a          568 c          558 g          376 t
ORIGIN      
        1 caatgcgcat gcgggcctga gcgatcagga caaggtggag atgcagcagt acatcacccg
       61 ctgctacggc accctcacca cgttcaacgt cctgttcgag gacaaggagg accagttcgt
      121 tgggcagaaa ggcgagtgat caacatcaga cgatttttca acgggtgttg cattttccgc
      181 tggagggtct accttcggca catcgttctt tgctgatcca cctgaagtga cttccatcca
      241 gcccgtccat tcgggcttga tcaacgcgaa gcccacacag gccatctgtt catcggaacc
      301 aacaatttcc atcagggatc gcaccctcgg tggtctacgg cgggcctcaa gccatcttcg
      361 gatggtctcc ttcgcggagc acgaggatta cggaaaccac catcgcgatc ccgcccatgt
      421 gatctcatgg gttccttgaa cgtggccctg tgtgggcttt tttttgcgga tcgcatgggc
      481 ctatgaacgc atgggcacat ctgcattcct ttgccccatg tcccgccttc ccgtcatgga
      541 atccttctac accctgcagg gcgaagggct tttcgcaggt caggcggcgt atttcatccg
      601 gctcggtggt tgcgatgtgg gttgtgcctg gtgtgatgtg aaggaaagtt gggacgcttc
      661 ggcccatccg accagagcgg tgaacgatat cgtgtccgag gcctcggcat accccgcacg
      721 catcgccgtg atcaccggtg gagaacccct gatgcatgac ctctcggaac tcaccgcttc
      781 attgaggtcg gccgggttcc gtacccacat cgagaccagc ggtacgcatc cgttcagcgg
      841 agattggcac catgtgtgct tgagcccgaa gaaattcaag gccgcgcttc ctggggcgta
      901 cccacgggcc gatgaactca aggtaatcgt cctcaacaga catgatctgc attgggcggt
      961 ggaacaggcg gccggtacgc gacccgactg cgtgttgtac atgcagcccg aatggagcaa
     1021 gcgcgaatcg gccatgccgc tgataacgga gcacatcaag gcgcatccgc gttggcgggt
     1081 atcattgcag acgcacaaat acatgaacat cccatgaggt tccgcttgac gccgatcttt
     1141 gagctggcag ttggcagcag gcagcaggca gccattcttg tccaggttgc actgctcgct
     1201 ctctgctgcc tgctgcctcc tggtgctttt gcacagcccg gcagctatac caccaccgac
     1261 aagagggcca tcaagctgta cgagagcggc ggcgattgca tgcgtcaacg caagtgggcg
     1321 tgcgctcagg aggatctgga gaaggcggcg aagcaggacc catcgttcat cgagccgcgt
     1381 atcctgctgg cggagatgta cgagcaacag gacaaggcga ccgatgcgat ggccgcgtgg
     1441 aacgaggtgc tcgccatttc gcccaagtac ttcaagcccg ccgcgttgca ccaggccgaa
     1501 cttcaattca ccgccgggaa gtatgacgac gccgagaaga actaccggct ctacctcacg
     1561 ttggatgatg acccactgcg caagtcccgg gcgttgctgg gcatcgagaa gtgcgctttc
     1621 gcgcgcgtgg ccgtgcagca tcccgtgccg ttcgagccga agaacctggg tccgggggtg
     1681 aacaccagtg atccagaata ctacccgtgc atcacggccg acgggaggac gttgctcttc
     1741 acccgccgcg tggccgaacc ttcggcccag tatggcatgc aggaggactt ctacatcagc
     1801 tacaagggcg atacggcctg gaacagggca tcccctgttc cttcggtgaa cacgaccacg
     1861 atgaatgaag gggcgggcac actcagcccc
//
LOCUS       DAOQOC010000642         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42648_length_1888_cov_3.297327, whole genome shotgun sequence.
ACCESSION   DAOQOC010000642 DAOQOC010000000
VERSION     DAOQOC010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42648_length_1888_cov_3.297327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..328)
                     /gene="rnpB"
                     /locus_tag="PL106_10225"
     ncRNA           complement(8..328)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PL106_10225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(354..944)
                     /locus_tag="PL106_10230"
     CDS             complement(354..944)
                     /locus_tag="PL106_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNA33485.1"
                     /translation="MSERVGIAVGDEVSFLDEEGGGTVLRMVDRGRVVVRTWDGFELE
                     YPVKLVVKREVGSKYTLSDHHAKLVADNDRLHDRIEKHKGRGASDRNGGRANQRVEDP
                     YTMEIDLHLHKLVEDESRLSDDAKLTFQLNYFERMLNSAIKLRKRRLVVIHGVGEGVL
                     REEVRKVLQFYEGCRFHDADPFRYGNGATVIDIVHY"
     gene            925..1728
                     /locus_tag="PL106_10235"
     CDS             925..1728
                     /locus_tag="PL106_10235"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNA33486.1"
                     /translation="MPTRSLTLRIYGLVITACSVLLFAVVPKRAMAQSFAAPEPFGGA
                     QAVKWLIEQEFVMAPEEVAMGTEGAVELAFTVQADGTPKNMRVSRTLGEANDKEALRV
                     ASLIRWFPASVGGSALDKEHTLLVPISAKRYKKAHAKDGPPPAFVALPADDSGMLYPD
                     KGLDSLAAPLIAKGLRGLQAYLAENLRYPEDARRRDIQGKVGVEFTVETSGHTANVRT
                     LDALGAGCDEEAMRLIRSIKWRPAIKDGKRVRSILKLDIVFRLDTNRRP"
BASE COUNT          365 a          629 c          536 g          358 t
ORIGIN      
        1 cctcgcgatg aaggctccat catttatcca ggatgcccct cgcggaacac ctcgatcgat
       61 ctacccgccg gcaggacggg ccgccctgtt gccggcctat ttgatctttc agcccgtggg
      121 gtttaccgaa atggcctcat cactgacgcc aagcgcgggc tcttaccccg cgttttcacc
      181 gttaccccgg caagccgggg cagtttccct ttctgtggca ctttccgtgg cacggccgtc
      241 gccagccttg ccccttcccg ttaggaagca cggtgccctg tgctgcccgg actttcctcc
      301 cccctccttg cgaagagcag cgatggagcg gtgcgcagga tgtcaatgaa ccttcaataa
      361 tgcacgatgt cgatcaccgt ggccccattt ccatagcgga acgggtccgc gtcgtggaag
      421 cggcagccct cgtaaaactg cagcaccttc cgcacctctt cgcgcagtac accctcgccc
      481 accccatgga tgaccaccaa gcgtcgcttc cgcaatttga tcgccgaatt cagcatccgc
      541 tcgaagtagt tcaactggaa ggtcagcttg gcatcgtccg atagccgcga ctcatcctcc
      601 accaacttgt gcaggtgcag gtcgatctcc atggtgtatg gatcctccac ccgctgattc
      661 gcgcggccgc cattgcgatc cgacgcaccc ctgcccttgt gcttctcgat gcgatcgtgc
      721 agccggtcat tgtcggccac cagtttggcg tgatgatcgc tgagggtgta tttcgagcca
      781 acttcgcgct tcacaaccag cttcaccgga tattccaatt cgaagccgtc ccaggtacgc
      841 accaccaccc gcccccgatc caccatgcgc aacaccgtgc caccgccctc ctcgtccaag
      901 aagctgacct catcgccaac ggctatgcct acgcgttcgc tcacgttgcg aatttacggc
      961 ctcgtgatca ccgcatgttc cgtcctgctc ttcgctgtcg tcccaaaacg cgctatggcc
     1021 caaagcttcg cggctccaga accttttggc ggtgcgcagg cggtgaagtg gctaatcgag
     1081 caggagttcg tgatggcgcc ggaggaggtc gccatgggta ccgagggtgc cgtggaactg
     1141 gcattcaccg tccaggctga cggcacaccg aaaaacatgc gtgtgagccg gacgctcgga
     1201 gaggcgaacg acaaagaagc cctgcgcgtg gcctcgctca tccgctggtt ccccgcctcg
     1261 gtgggaggca gcgcgctgga caaggaacac accctgctgg tgcccatcag cgcgaagcgg
     1321 tacaaaaagg cccacgccaa ggatggtcca cccccagcgt tcgttgcgct gcccgccgac
     1381 gactccggca tgctttaccc ggacaaaggt ctggactcac tcgcggctcc gctcatcgcg
     1441 aaagggcttc gtggcctgca ggcgtacctg gctgagaacc tgcgttatcc cgaggatgcg
     1501 cgacggcggg acatccaagg caaggtgggc gtggaattca ccgtggagac cagtggccac
     1561 acggccaacg tccgcacgct ggatgcgctg ggtgcggggt gcgatgagga agcgatgcgc
     1621 ctgatccgct ccatcaaatg gcgtcctgcg ataaaggacg gaaaacgtgt gcgaagcatc
     1681 ctgaaactgg acatcgtgtt ccggctcgat acgaaccggc gaccgtagcg catcttcgcc
     1741 gcatgaagaa acgcggccca tggaccacgc tgaaagagga gacgcgctat gagaccccgt
     1801 ggatccgggt gacgcaccac gacatcctcg accccagcgg acagcagggt atttacggga
     1861 cggttcattt caagaacctc gcgatcgg
//
LOCUS       DAOQOC010000643         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42674_length_1888_cov_2.444626, whole genome shotgun sequence.
ACCESSION   DAOQOC010000643 DAOQOC010000000
VERSION     DAOQOC010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42674_length_1888_cov_2.444626"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..564
                     /locus_tag="PL106_10240"
     CDS             13..564
                     /locus_tag="PL106_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA33487.1"
                     /translation="MVLQLANGDRPSAIAEGLGCDVSTVYRTRQAFLRRGEASLPPRK
                     SPGRPRRVTKQQECELDQALAREPRALGKNFSNWTAPNLKVHLHWTVQPVTVWRYLRR
                     LKWREVVGAKALVGDLDDRHQEALMLLDLMSKLNGTPFVEASAGIENIFKCLRVDGIW
                     RLTHRDAPRATNFALRMKLILNF"
     gene            561..1292
                     /gene="lptB"
                     /locus_tag="PL106_10245"
     CDS             561..1292
                     /gene="lptB"
                     /locus_tag="PL106_10245"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNA33488.1"
                     /translation="MMLRANDLVKRYKGRTVVGGVSVRVEQGEIVGLLGPNGAGKTTT
                     FYMVTGLVKPNSGGVFLDDQDITRLPMYKRAKLRIGYLPQEASVFRKLSVEDNIRAIL
                     EMTDLPKAGREEKLERLLSEFSLGHVRGNMGDVLSGGERRRTEIARALATDPRFILLD
                     EPFAGVDPIAVEDIQGIVSQLKKRNIGVLITDHNVQETLSITDRAYLLFEGRILKQGT
                     AEELAADEQVRKVYLGRNFELRRKA"
     gene            1324..>1888
                     /locus_tag="PL106_10250"
     CDS             1324..>1888
                     /locus_tag="PL106_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNA33489.1"
                     /translation="MAGRVFGLDLLRALAIVLVVLVHCSPWQLGRIDGIGWLFRIDGV
                     DLFFVLSGYLVGGLLLKAADRPDMAWRFRLLDFWQRRWLRTLPDYYVFLIINILLVAA
                     GLAPGMLSHATPAYAVFMQDVIKPLDLFFWESWSLSVEEWFYLLFPIIMIAVVKAFRM
                     PVRKAFLFTALVFIAVPPMVRASYLGQL"
BASE COUNT          327 a          553 c          641 g          367 t
ORIGIN      
        1 cgacgctgtc agattgtgtt gcaattggcc aatggtgatc gcccctcggc gattgccgag
       61 gggctgggct gcgatgtgtc gacggtctac cgtacgcgac aagcgtttct gcgccgaggc
      121 gaagccagtc tgccgcctcg gaaaagtccg gggcgaccac ggcgcgtgac caagcagcaa
      181 gagtgcgagt tggatcaggc gttggcacgt gagccgcgcg ccttgggcaa gaacttttcg
      241 aattggaccg cacccaatct gaaggtgcat ttgcactgga ccgtccagcc ggtcaccgtc
      301 tggcgatacc tgcggcggct gaagtggcgc gaagtggtgg gggccaaggc cctggtgggc
      361 gacctggacg accgacacca ggaggcgttg atgttgctgg acctgatgag caagctgaac
      421 ggcacgccgt tcgtggaggc cagtgcgggc atcgagaaca ttttcaaatg cctgcgtgtg
      481 gacggcatct ggcggttgac gcaccgcgat gcgccccgcg ccacgaactt cgccctgcgc
      541 atgaaactga tcctgaactt ctgatgttgc gggccaacga cctcgtgaag cgctacaagg
      601 gccgcaccgt ggtgggcggg gtgagcgtgc gcgtggagca gggcgagatc gtgggcctcc
      661 tcggtccgaa cggtgccggc aagaccacca ccttctacat ggtcaccggc ctggtcaagc
      721 ccaacagtgg cggcgtgttc ctcgacgacc aggacatcac ccggctgccg atgtacaagc
      781 gggccaagct gcgcatcggg tacctgccgc aggaggccag cgtcttccgg aagttgagcg
      841 tggaggacaa catccgggcg atactggaaa tgacggatct gcccaaggcc gggcgcgagg
      901 agaagctgga gcgtctgctg tccgagttca gtctgggcca tgtgcgcggg aacatgggcg
      961 atgtgctcag cgggggtgaa cgccgtcgca cggagatcgc gcgcgccctg gccacggatc
     1021 cccggttcat cctgctcgat gagcccttcg caggggtcga tcccatcgcg gtggaggaca
     1081 tccagggcat cgtatcgcag ttgaagaagc gcaacatcgg cgtactgatc accgatcaca
     1141 acgtgcagga gacgctcagt atcacggatc gggcctacct gctcttcgag ggcaggatcc
     1201 tcaaacaggg tacggccgag gaactggcgg cggacgaaca ggtgcgtaag gtgtacctcg
     1261 ggcggaattt cgaattgcgg cgcaaggcct gaacgcatgg tgcgtgcggg ggatcgggaa
     1321 cccgtggccg gcagggtatt cgggctggac cttctgcggg cactcgccat cgtgctggtg
     1381 gtgctcgtcc actgctcgcc gtggcagctc ggccggattg acgggatcgg gtggttgttc
     1441 cggatcgatg gggtggacct gttcttcgtt ctgagcggct acctcgtggg cggcctgctg
     1501 ctcaaggccg ccgaccggcc ggacatggcc tggcggttcc gcctcctgga cttctggcag
     1561 cgccgctggt tgcgtacgct tccggattac tacgtgttcc tcataatcaa tatcctcctg
     1621 gtggccgcgg ggctcgcccc gggcatgctc agtcacgcga cacccgccta cgccgtcttc
     1681 atgcaggatg tgatcaagcc gctggacctc tttttctggg agtcatggtc gctgagcgtg
     1741 gaagaatggt tctacctgct ttttccgatc attatgatcg cggtggtcaa ggcgttccgc
     1801 atgccggtac gaaaagcttt cctgttcacc gcgctcgtgt tcatcgctgt tccaccgatg
     1861 gtgcgagcct catacctcgg tcagttgc
//
LOCUS       DAOQOC010000644         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_42795_length_1885_cov_3.494536, whole genome shotgun sequence.
ACCESSION   DAOQOC010000644 DAOQOC010000000
VERSION     DAOQOC010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42795_length_1885_cov_3.494536"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="PL106_10255"
     CDS             complement(<1..1767)
                     /locus_tag="PL106_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33490.1"
                     /translation="MTRTVTLLATTILANTVFAALGGPDQYGYTWKDSNEPDGPVYEW
                     IDITTTGTQLTGFADDNVVGPFILGETFPFYWYTVKKIHIGSNGYLAFTTSGNIAAPF
                     PGIPLAGGTDNYVAGYMTDLNFAGEGNPGQLWVYDDMEQAVISYINVPYWNATAPGFW
                     TGSNTFQFVLDKTDSTITINYQNTLCCSGSNGPVCGIEALNGDFGLLQNSGTYPTSGF
                     SVRFYAPPTPLIDITDAAVEWITDPSNGGRAIPVNGAPFPMHAFLRNTGNQVLDPFTV
                     TNTIYDQANQTITVDAANVGELFPNATEDVYFSNSFVPDAPGTYRSVTTLSGIVGETV
                     ISNNSLTQEIVAYDTTLLYNDVDWAGAGDDGIGISWNGGAAGCGTYIIPPFYPINVSH
                     TTCRITNNIGNVGISMVVYDDDGPDGAPGTLLDSVSVQAMDAQAGDHAYPLTQPVVLD
                     SGGLYVLWYMNGANVSLAQDNVAPFSLRTYEVIQGAWAEYRDREVADFHLGLRMSIIP
                     EPDAAAVSFDQPADGQTLGSSTAVRIMIHNNGNLAVTGIPCGYSFNGGSPVLQTYNGP
                     AIAPGAEALFMFAQPLVPLADVT"
BASE COUNT          372 a          530 c          591 g          392 t
ORIGIN      
        1 cgtcacatcg gccagcggaa ccaagggttg cgcgaacatg aaaagggctt cggcaccggg
       61 cgcgatggct gggccgttgt atgtctgcaa aacgggagag ccgccattga agctgtatcc
      121 acagggaatg cctgtgaccg ctaggttgcc attgttgtgg atcatgatcc gcacggcggt
      181 gctggaaccc aaggtctgcc catcggctgg ttggtcgaag ctgaccgcag cggcgtcggg
      241 ttcggggatg atgctcatgc gcagaccgag gtggaagtcc gcgacctcgc gatcgcgata
      301 ctcggcccaa gccccctgga tcacctcgta ggtacgcaga ctgaaaggcg ccacgttgtc
      361 ctgcgccaag ctcacattgg ccccgttcat gtaccagagc acatatagtc cgccactgtc
      421 gagcacaaca ggctgtgtga gcgggtacgc atggtcaccc gcctgcgcat ccatggcctg
      481 cacagatacg ctgtccaaca gtgtaccggg ggcaccatcg ggaccatcat cgtcatacac
      541 caccatggaa atgcccacgt tcccgatgtt gttggtgatg cggcaggtgg tgtggctcac
      601 gttgatcggg tagaacggcg gaatgatgta cgtaccgcac ccggccgcac cgccgttcca
      661 gctgatgccg ataccatcgt caccagcgcc cgcccagtcc acatcgttgt acaacaaggt
      721 ggtgtcgtac gccacgatct cctgggtgag gctgttattg gaaatgacgg tctcccccac
      781 tatgccgctc aatgtggtca ccgagcgata ggtgccgggc gcatcgggca cgaaggagtt
      841 ggagaagtag acgtcctcgg tggcattggg gaagagttca ccaacgttgg cggcgtccac
      901 cgtgatggtc tggttggcct gatcgtaaat ggtattggtc accgtgaacg gatccagaac
      961 ctggttaccg gtgttgcgca ggaacgcgtg catggggaag ggcgcaccat tcaccggaat
     1021 ggcccggcca ccgttcgacg gatcggtgat ccactccacg gccgcatccg tgatgtcgat
     1081 cagcggtgtc ggcggagcat agaatcgtac gctgaatccg gaagtgggat acgtgcctga
     1141 attctgtagc aggccgaaat cgccgttgag tgcctcgatg ccgcacaccg gaccgttgct
     1201 cccactgcag cacagggtgt tctgatagtt gatggtgatg gtgctatcgg tcttgtccag
     1261 cacgaactgg aaggtgttgc taccggtcca gaaccccgga gctgtcgcgt tccaataggg
     1321 cacgttgatg tacgagatga cggcctgttc catgtcgtcg tatacccaca attggcccgg
     1381 gtttccctct ccagcgaagt tgaggtccgt catgtagccg gccacgtagt tgtccgtacc
     1441 gcctgccagc gggataccgg ggaagggtgc ggcgatgttt ccacttgtgg tgaacgccag
     1501 gtagccgtta ctgccgatat ggatcttctt cactgtgtac caatagaacg ggaaggtttc
     1561 acccaggatg aaggggccca ccacattatc atcggcaaaa ccggtgagtt gcgtgccggt
     1621 ggtggtgata tcgatccatt cgtagaccgg tccatccggc tcgttgctgt ccttccaggt
     1681 atagccgtac tggtctggtc cacccaaggc cgcgaagacc gtgttcgcaa ggatggtcgt
     1741 ggcgagaagg gtaactgttc ttgtcatggt tgccgagttg ttgccgaatg taggcgtatg
     1801 ggcgatccga ccaccgatcg ttcacggccg gaacatcccg agagccctcg atggagccga
     1861 tcaccctcgg ccggtgcgaa gggat
//
LOCUS       DAOQOC010000645         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43156_length_1877_cov_3.243139, whole genome shotgun sequence.
ACCESSION   DAOQOC010000645 DAOQOC010000000
VERSION     DAOQOC010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43156_length_1877_cov_3.243139"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PL106_10260"
     CDS             <1..297
                     /locus_tag="PL106_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33491.1"
                     /translation="IQQVRAWLGWRAELFYFRTHDGSELDLIIAQGLRPKAAIEIKTT
                     NSPVLSKGNHLAFEAVRAPVQLVLTPTAEDHAYTKGIEVCSMNTLWQKLDAALR"
     gene            complement(301..1362)
                     /locus_tag="PL106_10265"
     CDS             complement(301..1362)
                     /locus_tag="PL106_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA33492.1"
                     /translation="MAKKKAPAKTTKPTGPARKGKAATAKERVLITGAAGFLGSHLCD
                     RFLKEGYHVIGMDNLITGRLKNIEHLFKRSDFEFYHHDVSKYVHVPGQLKYILHFASP
                     ASPIDYLKIPIQTLKVSSLGTHNLLGLALQKKARILVASTSEVYGDPQVHPQTEDYWG
                     HVNTVGPRGVYDEAKRFQEAITMAYHTYHGLETRMVRIFNTYGPRMRLNDGRVLPAFI
                     GQALRGEDLTIFGKGNQTRSFCYVDDLIEGIYRLLKSDYSGPVNIGNPAEITIKQFAE
                     EIVKLTGTKQKLVYKPLPQDDPMQRQPDITLAKKILKWQPKVSRAEGLKITYDYFKSL
                     SPKELHEKDHNTFEGYVRK"
     gene            complement(1399..>1877)
                     /locus_tag="PL106_10270"
     CDS             complement(1399..>1877)
                     /locus_tag="PL106_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNA33493.1"
                     /translation="FRIIESVMQVNEDQKTALMPKILKHYNDPIKGKHFAVWGLAFKP
                     DTDDIREAPALYMIDALLAAGATVAAFDPEAMNNVKKLKGDSIRYATDEYDALQGADA
                     LILCTEWSEFRNPDMDRVKGALKRPVIFDGRNLYELETMKEKGFTYVSIGRNVVNG"
BASE COUNT          309 a          538 c          595 g          435 t
ORIGIN      
        1 atccagcaag tgcgcgcatg gctgggctgg cgcgcggaac tgttctattt ccggacccac
       61 gatggctccg agcttgacct gatcatcgcg caaggcttga gaccgaaggc ggccatcgag
      121 atcaagacca ccaattcacc ggtattgagc aaagggaacc acctggcctt cgaggcggtg
      181 cgagctccgg tgcaactggt gctgacgccc acggccgagg accatgccta tacgaaaggg
      241 atcgaggtgt gctcaatgaa caccctctgg cagaagttgg atgccgcgtt gaggtgaatt
      301 ctacttccgc acgtacccct cgaaggtgtt gtggtccttc tcgtggagtt ccttcggaga
      361 aaggctcttg aagtagtcgt acgtgatctt cagcccttcg gcacgcgaca ccttcggttg
      421 ccacttcagt atttttttcg ccagggtgat gtccggctgg cgttgcatcg ggtcgtcctg
      481 cggcagcggc ttgtacacca gcttttgctt ggtgcccgtg agtttcacga tctcctcggc
      541 gaactgtttg atcgtgatct ccgcaggatt gccgatgttc accgggcccg agtagtcgct
      601 cttcaggagg cggtagatgc cctcgatcag atcgtccacg tagcagaagc tgcgggtctg
      661 gttgcccttg ccgaagatgg tgaggtcctc accccgcagg gcctgaccga tgaaggcggg
      721 gagcacacgg ccgtcgttca agcgcatgcg cgggccgtag gtattgaaga tgcgcaccat
      781 gcgtgtttcc agaccgtggt aggtatggta ggccatggtg atggcctcct ggaaacgttt
      841 ggcctcgtcg tacacgccac gcgggcccac ggtgttcaca tggccccagt agtcctccgt
      901 ctgtggatgc acctgcggat caccgtacac ctcgctggtg ctggccacca ggatgcgcgc
      961 tttcttctgc agggcgaggc ccagcaggtt gtgcgtgccc agggagctca ccttcagcgt
     1021 ctggatcggg atcttcaggt agtcgatggg ggaggcgggg cttgcgaagt gcaggatgta
     1081 cttcagttgg ccggggacgt gcacgtattt gctcacgtcg tgatggtaga actcgaaatc
     1141 gctgcgcttg aacaggtgct cgatgttctt caggcgcccg gtgatcaggt tgtccatccc
     1201 gatcacatgg tagccttcct tcaggaatcg atcacagagg tgtgagccga ggaaaccggc
     1261 cgctccggtg atgagtacgc gctccttggc cgtggcggcc ttgcccttcc tggcgggacc
     1321 ggtcggcttc gtggtcttgg cgggtgcttt cttctttgcc atggatcagg tcggcccggt
     1381 ggggtcgacg gtacattgtt atccgttcac cacgttacgg ccgatgctca cgtatgtgaa
     1441 gcccttctct ttcatggtct ccagctcgta caggttgcgg ccgtcaaaga tcaccggtcg
     1501 cttcagcgcg cctttcacgc ggtccatgtc ggggttgcgg aattcgctcc actcggtgca
     1561 gaggatcagg gcatcggcgc cctgcaaggc gtcgtactcg tcggtggcgt agcggatact
     1621 gtcgcccttg agcttcttca cgttgttcat ggcctcgggg tcgaacgcgg ccacggtcgc
     1681 accggcggcg agcagcgcat cgatcatgta cagggcgggc gcctcgcgga tatcgtcggt
     1741 atcgggcttg aaggccaggc cccatacggc gaagtgtttc cccttgatcg ggtcgttgta
     1801 atgcttcagg atcttcggca tgagcgcggt cttctggtcc tcgttcacct gcatcacgct
     1861 ttcgatgatc cggaacc
//
LOCUS       DAOQOC010000646         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43292_length_1874_cov_3.263332, whole genome shotgun sequence.
ACCESSION   DAOQOC010000646 DAOQOC010000000
VERSION     DAOQOC010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43292_length_1874_cov_3.263332"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PL106_10275"
     CDS             complement(<1..445)
                     /locus_tag="PL106_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA33494.1"
                     /translation="MATTTQRIAILSFATFFLTSCNVVRMQARHTKKEFKKAGLAEHT
                     FAGPNGPMHVWSTSFTGKPKLMLVHGVTSSAAMWAGNAVPLSATYDLIIPDLIGHGGS
                     TNTWSGNSVDAQVAHLGAILDSLKVQEAVYVVGNSYGGAMAANLLA"
     gene            complement(472..675)
                     /locus_tag="PL106_10280"
     CDS             complement(472..675)
                     /locus_tag="PL106_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33495.1"
                     /translation="MQATPIGKDCYKIRLAISSKGGKSGGGRVVTYVRLLQGKVYLLT
                     IYDKTDQDSLTTKELLALLKGVE"
     gene            complement(784..1002)
                     /locus_tag="PL106_10285"
     CDS             complement(784..1002)
                     /locus_tag="PL106_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33496.1"
                     /translation="MEQIVLQLKNTRKRRLLLELLGELDFVKVTSVIRDGRKVAFAND
                     LLDALMEAKADARGEKKLTRARDFLKHG"
     gene            complement(1257..>1874)
                     /locus_tag="PL106_10290"
     CDS             complement(1257..>1874)
                     /locus_tag="PL106_10290"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HNA33497.1"
                     /translation="AGNSGIIGEGDVQWMTAASGVLHKEYHEKEFSRTGGTFHMAQLW
                     VNLPAKHKMEAPRYQAIKAGDMPEVQLPDGRSTAHILAGETFGAKGPAKTWSPIIAAV
                     IKLGAGGKADFELPAGFNSTILVVKGDAVINGAAAPVDNLVILANDGTGVEATSANGA
                     MLLVLSGEPLNEPIVAYGPFVMNTVEEIRQAMLDVQAGKFGVLED"
BASE COUNT          332 a          597 c          548 g          397 t
ORIGIN      
        1 gcgccagcag gttggccgcc atggcaccgc cgtaggaatt gcccaccacg tacacggctt
       61 cctgcacttt caagctatcg aggatggctc cgaggtgcgc cacctgcgca tccacgctgt
      121 tgccgctcca agtattggta ctgccgccgt ggccgatgag gtccgggatg atgaggtcgt
      181 aggtggcgct gagcggcacc gcgttgcccg cccacatggc cgcgctggag gtgaccccgt
      241 gtaccaacat cagcttcggc ttgccggtga acgatgtgct ccacacgtgc atggggccgt
      301 tgggtccggc gaacgtgtgc tcggcaagcc cggccttctt gaattccttc ttcgtgtggc
      361 gcgcttgcat cctcaccacg ttgcatgagg taaggaagaa cgtagcgaag gagaggatcg
      421 cgatgcgctg ggtagttgtt gccatgacga gtgatctatc cgtttctcga cctactcaac
      481 gcctttcaac aacgcgagca gttctttggt ggtgaggcta tcctgatcgg tcttgtcgta
      541 gatggtcaac aggtacacct tgccttggag aaggcgcacg taagtgacca cgcgtcctcc
      601 accggacttg ccgcccttgg acgaaatggc cagccggatc ttgtagcagt ccttgccgat
      661 gggtgtggct tgcaccggac aggctccaag gagcctacca attcaagcac atcagcatcc
      721 agcgagcgat gccgcttctt caactccttg aaccgcctct tgaagaaggc cgtgaggatg
      781 acctcagcca tgcttgagga aatcgcgggc gcgcgtgagc ttcttctcgc cacgggcatc
      841 ggccttggcc tccatcaacg catcgagcag atcgttggcg aaggccactt tgcggccgtc
      901 acggatcacg ctggtgacct tcacgaaatc caactcgccc agcagttcca gcagcaggcg
      961 gcgcttgcgc gtattcttga gttgaaggac gatctgttcc atcggttgct gaatacggag
     1021 cgaagttagt gcgccccaac ttccgatcca cgcaactgaa gaaccatcgc cggtcagtgg
     1081 gtggttggtt ccgggaaata atacgtgcgc tcggccagcc ccgccttctt gaattcctcc
     1141 ttcgcgtggc gtgccttatt cggcgatgcg gctcgatgcg atccaccgct cctcgttcac
     1201 ccggaaagtc gctggggccg tgcgtccacg gccccagcgc gttcgcactt gaacgatcag
     1261 tcctccagca cgccgaactt gccagcctgt acatccagca tggcctgccg gatctcctca
     1321 acggtgttca tcacgaaggg tccataggcc acgatgggtt cgttcagcgg ctcgccgctc
     1381 agcaccagca gcatggcgcc gttcgcactg gtcgcttcca caccggtgcc gtcattggcc
     1441 aggatcacca ggttgtccac cggggcggcc gcaccattga tcacggcgtc gcctttcacc
     1501 accaggatgg tactgttgaa cccggcgggc agttcgaagt cggctttgcc accagcaccc
     1561 aacttgatca ccgccgcaat gatcggcgac caggttttcg cgggaccttt ggcaccgaag
     1621 gtctcaccgg ccaggatgtg ggcagtgctc ctgccatcag gcagctgcac ctcgggcatg
     1681 tcgccggcct tgatggcctg gtaacgcggt gcctccatct tgtgcttcgc aggcaggttc
     1741 acccacaact gcgccatgtg gaaggttcca ccggtgcgtg agaattcctt ttcgtggtac
     1801 tccttgtgca gcacaccgct cgcggcggtc atccactgca catcgccctc gccgatgatg
     1861 ccgctgttgc cggc
//
LOCUS       DAOQOC010000647         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43330_length_1873_cov_4.580308, whole genome shotgun sequence.
ACCESSION   DAOQOC010000647 DAOQOC010000000
VERSION     DAOQOC010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43330_length_1873_cov_4.580308"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    179..278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            670..1338
                     /locus_tag="PL106_10295"
     CDS             670..1338
                     /locus_tag="PL106_10295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA33498.1"
                     /translation="MTGLDLLLKYFPDLSPEQRDRFMSLGPLYREWNERVNLISRKDI
                     EHLYERHVLHSLGIAKVLLESSSAPVARTMREGRLQRPWEGTRFIDVGTGGGFPLVPL
                     AVLFPQCIFHGIDGIGKKIMAVQGVIEGLGLQNCTAEQVRSEQHRERYDVIVSRAVTT
                     LPEFIRATKHLVRKGEGRMYYLKGGALADEILPVRNPVRVHDLNRWFPEEFFATKKVV
                     EVEM"
     gene            complement(1394..>1873)
                     /locus_tag="PL106_10300"
     CDS             complement(1394..>1873)
                     /locus_tag="PL106_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA33499.1"
                     /translation="EVLLFGGTIRENIAYGKPGATIQEVEDAARKANAHGFIASFPQG
                     YDTVVGERGIQVSGGQRQRIAIARAVLKDPAILILDEATSALDSESERLVQEALDALL
                     VGRTSIVIAHRLSTIRNADKILVLDKGHVVESGTHDELIADAEGLYHSLSRLQRTEA"
BASE COUNT          332 a          508 c          576 g          357 t
ORIGIN      
        1 gcacaggctt ccagcctgtg tggattgttg caggagcaca ggcttccagc ctgtggaaga
       61 gtgttgcagg agcacaggct tccagcctgt gaaagagtgt tgcaagggca caggtttcca
      121 gcctgtgtgg attgttgcag gagcacaggc tggaagcctg tggaagagtg ttgcaggann
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc acaggcttcc agcctgtgtg
      301 gattgttgca ggagcacagg cttccagcct gtgtggattg ttgcaggagc acaggcttcc
      361 agcctgtgga agagtgttgc aggagcacag gcttccagcc tgtgaaagag tgttgcaagg
      421 gcacaggttt ccagcctgtg tggattgttg caggagcaca ggctggaagc ctgtggaaga
      481 gtgttgcagg agcacaggct tccagcctgt gtggattgtt gcaggagcac aggcttccag
      541 cctgtggaag agtgttgcag gagcacaggt ttccagcctg tgcagaaaaa aatagaagcg
      601 ccaggggcac aggcttccaa cctgtgcgga tgtccttcgc aaaaccgaca acaagaagcg
      661 tattccgcga tgaccggtct ggatctcctc ctcaaatact tcccggacct gtcaccggag
      721 cagcgcgatc gcttcatgtc gctcggtccg ttgtaccggg agtggaacga gcgcgtgaac
      781 ctcatctcgc gcaaggacat cgagcacctc tatgaacggc atgtgctgca ttcgctcggt
      841 atcgcgaagg tgctgctgga aagctcgtcc gcgcctgtgg cgcggacgat gcgggaaggg
      901 cgcttgcagc gcccatggga aggtacacgt ttcatcgacg tgggtacggg cgggggcttc
      961 ccgctcgtgc cgctggccgt gctgttcccg cagtgcatct tccacggcat cgacggcatc
     1021 ggcaagaaga tcatggccgt gcagggcgtc atcgaagggc tcgggcttca gaattgtacc
     1081 gctgaacagg tgcgtagcga gcagcaccgg gagcgttacg atgtcatcgt gagccgcgcc
     1141 gtcaccaccc tgccggagtt catccgcgcc acgaagcacc tggtacggaa aggggagggg
     1201 cgcatgtact acctcaaggg cggcgcgctg gccgatgaga tcctgcccgt gcgcaacccc
     1261 gtacgtgtgc atgacctgaa caggtggttc cccgaggaat tcttcgccac gaaaaaggtg
     1321 gtggaggtgg agatgtagct ccgcacgggc ggtccagtac accggtgcat ccgggcgggc
     1381 cgtcatggcc cgatcatgct tccgtccgct gcaaccggct caagctgtgg tacagcccct
     1441 ccgcatcggc gatcaattcg tcgtgcgtgc cgctttccac cacgtggccc ttgtccagga
     1501 caaggatctt gtcggcattt cggatggtgc tgagccggtg cgcgatcacg atgctcgtgc
     1561 ggcccaccaa caatgcgtcc agcgcctcct gtacgagcct ctcgctctcg ctgtccagtg
     1621 cgctggtggc ctcatccagg atcaggatcg cggggtcctt aagcaccgca cgtgcaatgg
     1681 cgatgcgctg gcgctgccca ccgctcactt gtatgccacg ttccccgacc acggtatcat
     1741 atccttgcgg gaaggaggcg atgaacccgt gcgcattggc tttgcgggcg gcgtcctcca
     1801 cctcttggat cgttgcgccc ggcttgccgt aggcgatgtt ctcgcggatg gtcccaccga
     1861 acaggagcac ttc
//
LOCUS       DAOQOC010000648         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43337_length_1873_cov_3.673267, whole genome shotgun sequence.
ACCESSION   DAOQOC010000648 DAOQOC010000000
VERSION     DAOQOC010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43337_length_1873_cov_3.673267"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1402)
                     /locus_tag="PL106_10305"
     CDS             complement(125..1402)
                     /locus_tag="PL106_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA33500.1"
                     /translation="MIPGEPILIISHTFPPYKGIGGRRWAKFAKELARRGHPVHVVHS
                     AGSEDLMGSLWTDDADRPGITAHPLPQRYPTVLFKRPLTTIGAKVMYRVWSRLLPLIT
                     RGNWLDKSVLWRKQLVDTCTALIRGHNIHHVVVTGAPFGLMAHTCALRGVFPDLKLVA
                     DFRDPWTWGHYYGQGVLSPRRKAEEQRREAEVARVFNKLISPAPDIVEHLRHTYGGDP
                     ARYILLPHAIDPEEMGTGPIARNDNEYRMIYAGSLYGAEEAEAYFEEVLRAFERLRDK
                     DPERSARTFFDLYITGHGTEAYRAKAEARGLGAHIRFHAPLPPKAIFPLIAAADLVVI
                     FIPTPNKDFLGTKFNEIFYLRKPVLHVGAEGRVGRTITERGLGASIRVDEVAYELPRI
                     IRGERTIAIDPDADLSGHLLSHITDRLVREVLA"
     gene            1454..>1873
                     /locus_tag="PL106_10310"
     CDS             1454..>1873
                     /locus_tag="PL106_10310"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA33501.1"
                     /translation="MTHVRRILITGGAGFIGSHLVDRLLAEGGWQVNVLDNFDPFYPR
                     AIKESNIAAHRKHPAYHLVEGDILDDAALDRAFDPAPEVIVHMAAKAGVRPSIIDPIG
                     YHRVNVTGTLKLLERARHLGVKHFVLASSSSVYGTDPD"
BASE COUNT          318 a          587 c          613 g          355 t
ORIGIN      
        1 ccggaggtcc gcaccagaat gggggctttc agctttgacc gtgtgaagga gatcttcagc
       61 tggccggtgg tgacggagcg cttcgtgaag ttgttcgagg acgtggccct gccgcgttga
      121 tcgttcaggc cagcacctcg cgtacgagcc tatcggtgat gtggctgagc agatgcccgc
      181 tcagatccgc atccgggtcg atcgcgatgg tccgctcccc ccggatgatg cgcgggagtt
      241 cgtacgccac ctcgtccaca cggatggatg cgcccagtcc tcgctctgtg atcgtgcgtc
      301 ccacacggcc ctcggcaccc acgtgcagca caggtttccg caagtagaat atctcgttga
      361 atttggtgcc caggaagtcc ttgttgggcg tggggatgaa gatgaccacc aggtcggccg
      421 cggcgatgag cgggaagatc gccttcgggg gcagcggcgc atggaagcgg atgtgagcac
      481 ccagcccccg cgcctcggcc ttcgcccggt acgcctccgt gccgtggccg gtgatgtaca
      541 gatcgaagaa tgttcgtgct gatcgttcgg gatctttatc gcgcagccgc tcgaaggccc
      601 gcaacacctc ctcgaagtac gcttcggcct cttcggcccc gtacaggctg cccgcataga
      661 tcatgcggta ttcgttgtcg ttccgtgcga tgggaccggt ccccatctcc tccggatcga
      721 tcgcatgggg gagcaggata taacgcgcgg gatcccctcc gtacgtatgc cgcaggtgct
      781 caacgatgtc cggcgcgggg gagatgagtt tgttgaagac ccgggccact tccgcctcgc
      841 gccgttgttc ctcggctttc cgacgcgggc tcagcacgcc ttggccatag tagtgccccc
      901 aggtccaggg gtcgcggaag tccgccacca gcttcaggtc cgggaacacc ccacgcaagg
      961 cacaggtatg ggccataagc ccgaatggtg cgccggtgac caccacgtgg tggatgttgt
     1021 gtcctcggat gagcgccgta caggtatcca ccaactgctt gcgccacagt accgacttgt
     1081 ccagccagtt gccccgggtg atcagtggca gcaatcgcga ccacacgcgg tacatcacct
     1141 tcgcgccgat ggtggtgagc ggcctcttga aaagtacggt gggataccgc tgcggcagcg
     1201 ggtgtgccgt gatgccgggg cggtcggcat catccgtcca gagcgagccc atgaggtctt
     1261 cactgccggc gctgtgcacc acatgcaccg ggtgcccacg ccgggccagc tctttggcga
     1321 acttggccca gcgccgcccg ccgatgccct tgtagggcgg aaaggtgtgg ctgatgatga
     1381 ggatcggttc gccgggtatc atgggcgggc gaaagtatcc cgagcggggc cgaggggtac
     1441 attcgcggcc cgcatgaccc atgttcgcag gatactgatc accggggggg ccgggttcat
     1501 cggcagtcac ctggtggacc gcctgctggc cgagggaggc tggcaggtga acgtgctgga
     1561 caacttcgac ccgttctacc cgcgcgccat caaggagtcc aacatcgcgg cccacaggaa
     1621 gcatccggcg taccacctcg tggagggtga tatcctggac gatgccgcac tcgatcgcgc
     1681 ttttgatccc gcaccggagg tgatcgtcca tatggcggcc aaggccggtg tgcggcccag
     1741 catcatcgat cccatcggct accaccgtgt aaatgtgacc ggcacgctca agctgctcga
     1801 gcgtgcgcgc catctcggcg tgaagcattt cgtgctcgcc agcagcagca gtgtgtacgg
     1861 tacggatccc gat
//
LOCUS       DAOQOC010000649         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43378_length_1872_cov_3.533847, whole genome shotgun sequence.
ACCESSION   DAOQOC010000649 DAOQOC010000000
VERSION     DAOQOC010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43378_length_1872_cov_3.533847"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1872
                     /locus_tag="PL106_10315"
     CDS             <1..>1872
                     /locus_tag="PL106_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNA33502.1"
                     /translation="VQAGAAYVPIDPTYPAERIAGMLEDAKPPVVITCKAHQHLFKGT
                     TAKVLLIEDIDLKNGPLFEGPCPAGPDDLLYVLFTSGSTGRPKGVAMHNAPLTNLIQW
                     QLRTSVLKEGDRTLQFAPISFDVSFQEIFATFAQGGTLVLITDEDRLNSTQLLRKIIA
                     EKINRIIVPFVALQYLAEAVERTGEIPSSLKEVFTSGEQLKITPAIINLFKQLPGARF
                     CNQYGPTEGHVVSELELKGDPSTWPALPNIGKAIDNVKLYVLDEQMKPVKKGEEGELY
                     LGGACVAKGYIGRDDLTAERFLADPFVSGGRMYKTGDRAAELPNGEIDYKGRIDGQVK
                     VRGYRIELGEVEVAMEKHPAVEQAVANVREDRPGLKRLIGYYVAKSELSTNELRKHLA
                     SLLPDYMQPSAFVAVKELPRTPSGKIDRKALPAPDVKRPELDVAFAAPQSTVQKTLAH
                     VWADLLGIDRVGIDDNFFDLGGNSLLSIQCVAQLEGHGLKLPIVKLYQHPTIRACAAF
                     LEGDASAVSPAEMAKARVGAKHVSPQWDIAIIGMSGRFPGAANVEELWRNLLAKKNSI
                     STWTAEEIDPSVPAELRNDPDYVKARGVITDADKFDHGFFGVNPKVAALMDPQQRVFL
                     "
BASE COUNT          394 a          598 c          568 g          312 t
ORIGIN      
        1 gtgcaagccg gtgccgccta cgtgcccatc gaccccacct accccgccga gcgcatcgcc
       61 ggcatgttgg aggacgccaa gccgccagtg gtgatcacct gcaaggcgca ccagcacctc
      121 ttcaagggca ccacggccaa ggtgctgctg atcgaggaca tcgacctgaa gaacggacct
      181 ttgtttgaag gcccatgccc cgcaggtccc gacgacctgc tctacgtgct gttcaccagt
      241 ggcagcactg gccgccccaa gggcgtggcc atgcacaatg cgccgctcac caacctgatc
      301 caatggcagc tccgcacctc ggtgttgaag gaaggcgatc gcaccctgca gttcgcgccc
      361 atcagcttcg acgtgagctt ccaggagatc ttcgccacgt tcgcccaagg cggcacattg
      421 gtgctgatca ccgatgagga ccgcctgaac agcacgcaac tcctgcgcaa gatcatcgcc
      481 gagaagatca accgcatcat cgtgcccttc gtggcgctgc aatacttagc cgaagcagtt
      541 gaacgtaccg gcgaaatacc ttcttcattg aaagaggtct tcaccagtgg tgagcagttg
      601 aagatcaccc cggcgatcat caacctcttc aaacaactgc ccggcgcgcg cttctgcaac
      661 cagtacggcc ccaccgaagg ccacgtggtg agcgaactgg aactgaaagg cgacccgtcc
      721 acctggcccg cactgcccaa catcggcaag gccatcgaca acgtgaagct gtacgtgctc
      781 gatgagcaga tgaagcccgt gaagaagggg gaagaaggtg aactctacct cggcggtgcg
      841 tgcgtggcca agggctacat cggtcgcgat gaccttaccg ccgaacgttt cctcgcggat
      901 cctttcgttt ccggcggtcg catgtacaag accggcgacc gcgcggcgga gctgcccaac
      961 ggcgagatcg actacaaagg ccgcatcgac ggccaggtga aggtgcgtgg ctaccgcatt
     1021 gaactgggcg aggtggaagt ggccatggag aagcaccctg ccgtggagca ggccgtggcc
     1081 aacgtgcgcg aggaccgccc aggtctgaag cgcctgatcg gctactacgt ggcgaagagc
     1141 gagctcagta ccaacgagct gcgcaagcac ctcgcctctc tcctgcccga ctacatgcag
     1201 ccttcggcct tcgtggccgt gaaggaactg ccccgcacac ccagcggcaa gatcgaccgt
     1261 aaggcattgc ctgcacccga tgtgaaacga cccgagttgg acgtggcctt cgctgcgcca
     1321 cagagcaccg tacagaaaac gctcgcccat gtgtgggccg atctgctcgg catcgaccgc
     1381 gtgggcatcg acgacaactt cttcgacctc ggcggcaact ccttgctgag catccaatgc
     1441 gtggcgcagt tggaaggaca tggcctgaag ctgccgatcg tgaaattgta ccagcatccc
     1501 acgatcaggg cgtgtgcggc gttcctggag ggcgatgcga gcgccgtgtc ccctgccgag
     1561 atggcgaagg cccgcgtcgg ggcgaaacat gtctcgcccc agtgggatat cgccatcatc
     1621 ggcatgagcg ggcgttttcc tggtgccgcg aacgtggagg aactgtggcg gaacctgctg
     1681 gccaagaaga acagcataag cacctggacc gccgaggaga tcgacccgag cgtgcctgcg
     1741 gaactgcgca atgatccgga ctatgtgaaa gcgcggggtg tgatcaccga tgcggacaag
     1801 ttcgaccacg gcttcttcgg cgtgaacccc aaggtggccg cgctgatgga tccgcagcaa
     1861 cgcgtgttcc tg
//
LOCUS       DAOQOC010000650         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43385_length_1872_cov_2.837644, whole genome shotgun sequence.
ACCESSION   DAOQOC010000650 DAOQOC010000000
VERSION     DAOQOC010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43385_length_1872_cov_2.837644"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1077
                     /locus_tag="PL106_10320"
     CDS             148..1077
                     /locus_tag="PL106_10320"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA33503.1"
                     /translation="MGKNVLITGGAGFIGSALSKQLQEDGHAVAVLDNLSFGRRHLAG
                     VLDDRFFEVDIRAREATRKVLADTRPEIVLHLAAVHFIPYCNQHPVEAADININGTIN
                     VLDAAQAIESVEQVFVASTAAVYPIADGAMDEQHVLGPMDIYGTTKLATEKLASEFHL
                     RTGIPTIVGRFFNAFGPNETNPHLFPEIQRQVLSGARTLKLGNLDPKRDYIHTEDMSR
                     AMSALLSKGVSGYDVFNIGRGIEYSVREIVAAFERQLGEPLTIEVDPARVRKVERQHL
                     LADVRKLKAATGWEPKWDIDQGVATLLTEHVEQ"
     gene            1130..>1872
                     /locus_tag="PL106_10325"
     CDS             1130..>1872
                     /locus_tag="PL106_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA33504.1"
                     /translation="MQRHSRLLAEHLARSGRVELTVLHPHPVGIFDPALGIREVHVPD
                     IDRTKFYLRELWRYSDRTGKELETLGADVILSQGFCVWKGIDRFSHRLIVHPHGLEMF
                     QMLTRKERVLGWPFRAALKHIVRRGAVVISLGGKLTGILHDLAAGSKCRVVVLPNATD
                     VPAAPPAPPPPTGPLKLLFVGRFAFNKGLDVLMAVAERLVKEGLQDRVRFQLAGDGPL
                     LEIYQRTGLPVNVELLGRVDDARLSELYAT"
BASE COUNT          357 a          575 c          600 g          340 t
ORIGIN      
        1 ataagagcat gtgcccggcc cggaagggct gatcgggact tccgaccctc tggtcctcgg
       61 agctgtcaac ggccggtcag ctctccgcga ggatcggacc gttggcgaac caccgtgcgg
      121 tgagcttgcc ctgtactttc gcgcaccatg ggcaagaacg tcctaatcac cggcggtgcc
      181 ggtttcatcg gctcagcgct cagcaagcaa ttgcaggagg atggccatgc ggttgccgtg
      241 ctcgacaacc tgagcttcgg ccgtaggcat ctggccggag tgttggacga tcgcttcttc
      301 gaagtggaca tccgggcgcg cgaggcgacc cgaaaagtgc tggccgatac gcgtccggag
      361 atcgtgctcc acctggccgc tgtccacttc atcccatact gcaaccagca ccccgtggaa
      421 gcggctgaca tcaacatcaa cggtacgatc aatgtgctgg acgccgcgca ggccatcgag
      481 agcgtggagc aggtcttcgt ggcgagcact gcggccgtgt accccattgc cgatggcgcc
      541 atggacgagc agcatgtgct ggggcccatg gacatttacg ggaccaccaa gctggccacc
      601 gagaagctcg ccagtgaatt ccacctgcgc acggggatcc ccaccatcgt gggtcgcttc
      661 ttcaacgcct tcggaccgaa cgaaactaat ccgcatctct ttccggagat ccaacgccag
      721 gtgctcagtg gagcgcgtac gttgaagctg ggcaacctcg atcccaagcg ggattacatc
      781 cacaccgagg acatgagccg cgccatgagc gccctgctca gtaaaggcgt atcgggatac
      841 gatgtgttca atatcgggcg gggcatcgag tacagcgtgc gcgagatcgt ggccgcattc
      901 gaacgtcaac tcggcgagcc gctcaccatc gaggtggacc cggcgcgcgt gcgcaaggtg
      961 gagcgtcagc acctgctggc cgatgtgcgc aagttgaagg ccgcaacagg ctgggagccg
     1021 aagtgggata tcgaccaggg ggtagcgacc ctgctcacgg agcatgtcga acagtaagct
     1081 ccgcatcgcg ctctgcacgg acggcgtcca tccacatgca atgggtggaa tgcaacgtca
     1141 ttcgcggctg ctggccgagc atctggcacg ttcgggccgg gtggaactca ccgtgctgca
     1201 cccgcatccg gtcgggatct tcgatccggc cctgggcata cgtgaggtgc acgtgcccga
     1261 catcgacagg accaaattct acctgcgcga actgtggcgg tacagcgacc ggacagggaa
     1321 ggaactcgag accttggggg ccgatgtgat cctttcgcag ggtttctgcg tgtggaaagg
     1381 tatcgaccgc ttcagccatc ggctgatcgt tcacccccat ggactggaga tgttccagat
     1441 gctcacccgc aaagagcgcg tgctgggctg gcccttccgg gcggccttga aacacatcgt
     1501 tcgacgcggt gcggtggtga tcagcctcgg gggcaaactc acaggcatat tgcacgatct
     1561 ggcggctggg agcaagtgcc gggtggtcgt cctgcccaac gcgaccgatg tccctgccgc
     1621 gccgcccgct ccaccgcccc cgaccgggcc gctcaagctg ctcttcgtgg gccgcttcgc
     1681 cttcaacaag gggctggatg tcctgatggc cgttgccgaa cgactggtga aggaagggtt
     1741 gcaggaccgc gtacgcttcc aactcgctgg cgatggtccc ctgctggaaa tataccagcg
     1801 cacaggtctt ccggtcaacg ttgagttgct gggaagggtg gatgatgcgc ggctcagcga
     1861 actgtatgcg ac
//
LOCUS       DAOQOC010000651         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43394_length_1872_cov_2.543203, whole genome shotgun sequence.
ACCESSION   DAOQOC010000651 DAOQOC010000000
VERSION     DAOQOC010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43394_length_1872_cov_2.543203"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PL106_10330"
     CDS             <1..247
                     /locus_tag="PL106_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNA33505.1"
                     /translation="WIHQNAWFHMGTFDKDFKLSYDLKDRRNGVYAFVIKGDITVNDT
                     ALNERDGLGVWDAAALTIEANSQDAELLLMEVPMQLN"
     gene            492..1574
                     /locus_tag="PL106_10335"
     CDS             492..1574
                     /locus_tag="PL106_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HNA33506.1"
                     /translation="MNLFRNFKSVTRTAYGRGSFGKLGEIIDPQRGAGNGYMVFLVDH
                     YFQNNSAFLARIPALPGDEVLFCSTAKEPSTGQIDALRDDLLARRGLPAGLVGIGGGN
                     VMDVAKALSLMFTNEGSSVQYQGLNLIKKPGIYHCGVPTISGTGAEVSMTAVLTGPVK
                     KLGLKCDWTVFDQIVLDPDLIATVPDDQWFYTGMDTYIHSVEAITGTKKNTFAEAYSE
                     KSLEMCREVYLRLDRKDPKSDELLMVASYMGGLSLTYSEVGVCHALSYGLGKVLEIHH
                     CYANCIAFNQLEDVYGAHVQEFKGMVKERGISLPQGLAKDWSEETISAMAEVAYALPH
                     MWDHAFGPGWQQVLDRERIKGWYRRM"
     gene            complement(1657..>1872)
                     /locus_tag="PL106_10340"
     CDS             complement(1657..>1872)
                     /locus_tag="PL106_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33507.1"
                     /translation="MSLNDIRQAYNLPPGLLSVFNQDLRTRNRGKSCVRFRKLHDEDL
                     ELIDRMVLYVGTIFALEPQGARKVERV"
BASE COUNT          379 a          571 c          571 g          351 t
ORIGIN      
        1 gtggatccac cagaatgctt ggttccacat gggtaccttc gacaaggact tcaaattgag
       61 ctacgacctg aaggacaggc gcaacggggt atacgccttc gtcatcaaag gcgatatcac
      121 cgtcaatgac accgcgttga acgagcgcga tggcctcggc gtgtgggatg ccgcagcact
      181 gaccatcgag gccaacagcc aggacgccga actgctcctg atggaagtgc ccatgcagtt
      241 gaactgacct cgggccaccg gcgtcctgcc tgcgatcatc ctgggccacc ggcatcctgc
      301 cggtgagcat cgacccggaa cgaacaaagc caccggcttc cagtaacctt cccgggtcac
      361 cggcatcctg cccgaggtcc aaactccagg tcatggtccg cacggtacat cttccaccgg
      421 ccggtctttc ctttgtaccg cccatccggc gccccgaccc gccatccact gataccatga
      481 tccactgatc aatgaacctg ttccgcaact tcaagtccgt taccaggaca gcgtacggcc
      541 gtggctcctt cggcaaactc ggcgagatca tcgatccgca acggggcgcg ggcaatgggt
      601 acatggtgtt cctggtggac cactacttcc agaacaacag cgcattcctg gcgcgcatcc
      661 cggcccttcc cggcgacgag gtcctgttct gcagtaccgc caaggagccg tccaccggac
      721 agatcgatgc cctgcgcgat gatttgctgg ctcggcgtgg gctgcctgcc ggcctcgtcg
      781 gtatcggtgg gggcaacgtg atggacgtgg ccaaagcact ttctctgatg ttcacgaatg
      841 aggggagcag cgtgcaatac cagggcctca acctgatcaa gaagcccggt atctaccact
      901 gcggcgtgcc caccattagc ggtaccggtg cggaggtgag catgaccgcc gtcctcaccg
      961 gcccggtgaa aaagctcggg ctcaaatgcg actggaccgt cttcgatcag atcgtgctcg
     1021 accctgacct catcgccacc gtgcccgatg atcagtggtt ctacaccggc atggacacgt
     1081 acatccacag cgtggaggcc atcaccggca ccaagaagaa caccttcgcc gaggcgtaca
     1141 gcgaaaaaag ccttgagatg tgccgggagg tgtacctgag gctggatcgc aaggacccga
     1201 agagcgatga gctgctgatg gtggccagct acatgggtgg gctcagcctg acgtacagcg
     1261 aggtgggcgt ttgccacgcg ttgagctacg gtttgggcaa ggtgctggag atccaccact
     1321 gctacgcgaa ctgcatcgca ttcaatcaac tggaggatgt ctatggtgct catgtgcagg
     1381 agttcaaggg catggtgaag gagcgtggca tctcattgcc ccaggggctg gccaaggatt
     1441 ggagcgaaga gaccatcagc gcgatggccg aggtggccta tgcactaccg cacatgtggg
     1501 atcacgcctt cggacccggt tggcagcagg tgctcgaccg tgaacggatc aaagggtggt
     1561 accgcaggat gtgatcgttc acgggcctgt tcggaacggg gcgcatgccc ggagtgctgc
     1621 agcgggcgcg aggttacctg ggccggcgcc ggtgcgtcat acacgctcga ccttcctggc
     1681 gccctgcggt tccagcgcga atatggtgcc aacgtacagc accatccgat cgatcaattc
     1741 caggtcctca tcgtgcagct tgcggaagcg cacacaactc ttgccccggt tcctggtgcg
     1801 caggtcctgg ttgaaaacgc tgagcaggcc gggtggcagg ttgtatgcct ggcggatgtc
     1861 gttcaggctc at
//
LOCUS       DAOQOC010000652         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43423_length_1871_cov_3.887115, whole genome shotgun sequence.
ACCESSION   DAOQOC010000652 DAOQOC010000000
VERSION     DAOQOC010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43423_length_1871_cov_3.887115"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PL106_10345"
     CDS             <1..457
                     /locus_tag="PL106_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA33508.1"
                     /translation="KLTFAPSRHFTGRAMTNRFSTLWGSFVIEGRSRRLYFGADSGYS
                     PTFKELGERFGSFDLVMLECGAYSEYWPDIHMFPEETVQAAQDLRAAALMPIHWGKFA
                     LGLHPWKESIERVTKKADELGLPLLTPRIGRIITVLDRSSSEKWWEGLK"
     gene            528..1742
                     /gene="dinB"
                     /locus_tag="PL106_10350"
     CDS             528..1742
                     /gene="dinB"
                     /locus_tag="PL106_10350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNA33509.1"
                     /translation="MDRNILHLDLDSFFVSVEVLKHPELKGRPVLIGSDSDRGVVASC
                     SYEARALGIRSAMPMRMAKQLCPEAIILRGNSGAYMKKSDEVTEIIREQVPLFEKSSV
                     DEFYVDFTGTEKFFGAMKLATELRQRISKETGLPNSFGLSINKTVSKVATNQAKPNGQ
                     LQVERGTEKPFLAPKPIEAIPGVGEKTAHLLRSMGVARIHTLQELPIDLMQRLLGEHG
                     PVLWRKANGIDNSPVVAWHERKSISTERTFERDTIDVVKLKGLLTAMAESLAFQLRKG
                     AKLTSCVAVKVRYADFDTHTQQQRIGYTACDHIILPVVHELFRKLYDRRQRIRLIGVR
                     FSHLVGGGHQMDAFTDTQEAMNLYQAMDRIRKRFGDRTVMRASGMEARSIGRMDNPFD
                     GQAPVLLANRRS"
BASE COUNT          376 a          605 c          592 g          298 t
ORIGIN      
        1 gaagctcacc ttcgccccat cgcgccactt cacgggcagg gcgatgacca accgcttcag
       61 caccctgtgg gggtcatttg tgatcgaggg gaggagcagg cgcctctact tcggggccga
      121 cagcggctac tcaccgacgt tcaaggaact cggcgaacgc ttcggatcct tcgatctggt
      181 gatgctggag tgcggtgcct acagcgaata ctggcccgac atccacatgt tccccgagga
      241 aacggtacag gccgcacagg atctgcgggc cgcggcgctc atgcccattc actggggcaa
      301 gttcgctttg ggcctgcatc cgtggaagga gtccatcgag cgggtgacga agaaggcgga
      361 cgaactcggc ctgcccttgc tcaccccgcg catcgggcgc atcatcaccg tgctggaccg
      421 gtcatccagc gagaagtggt gggaagggtt gaagtgagtg cgaggcattc taccttatcg
      481 gccagcggat cgacacggaa atcgtcgatc gcgatcatcc gctcatcatg gaccgcaaca
      541 tcctccacct ggatctggac agcttcttcg tgagcgtgga ggtgctcaag catccggagc
      601 tgaaaggcag gcctgtgctc atcggctccg acagcgatcg cggcgtggtg gccagttgca
      661 gctacgaggc gcgggccttg ggcatccgca gtgccatgcc catgcgcatg gccaagcagt
      721 tgtgccccga ggcgatcatc ctgcgcggca acagcggggc ctacatgaag aagagcgacg
      781 aggtcaccga gatcatccgc gagcaggttc cattgttcga gaaaagcagc gtggatgaat
      841 tctacgtgga cttcacgggc accgagaaat tcttcggggc catgaagctc gccacggaac
      901 tgcgccagcg catcagcaag gaaacggggc tgcccaacag cttcggcctc tcgatcaaca
      961 agacggtgag caaggtggcc accaaccagg ccaagcccaa cggccagttg caggtggagc
     1021 gcggcacgga aaagcccttc ctcgcgccca agccgatcga agcgatcccc ggggtagggg
     1081 agaagaccgc gcacctgttg cgcagcatgg gcgtggcgcg catacacacc ctacaggaac
     1141 tgccgatcga cctgatgcag cggctgctgg gcgagcacgg ccccgtgctc tggcgcaagg
     1201 ccaacggcat cgacaacagc ccggtggtgg cctggcacga acgcaagagc atcagcaccg
     1261 agcgcacctt cgagcgcgac accatcgacg tggtgaagct caaggggctg ctcaccgcca
     1321 tggccgaaag cctcgccttc caactgcgca aaggtgccaa gctcaccagt tgcgtggccg
     1381 tgaaggtgcg ctacgccgac ttcgatacgc acacgcagca gcaacgcatc ggctacacgg
     1441 cctgcgacca catcatccta ccggtcgttc atgaactttt ccgcaagctg tacgatcgcc
     1501 gccagcgcat ccgcctcatc ggtgtgcgct tcagccacct ggtgggcggt ggccaccaga
     1561 tggacgcctt caccgatacg caggaggcca tgaacctcta ccaggccatg gaccggatcc
     1621 gcaagcgctt cggcgaccgc accgtgatgc gcgccagcgg catggaggcc cgcagcatcg
     1681 gccgcatgga caaccccttc gatgggcagg cgccggtgct gctggcgaac aggaggagtt
     1741 gaacaggact caccacatgc ggttctcttg ggcgtgtccc cgctcaagag cagcggggcc
     1801 gggtgctccg ctatagctgc ctcggcccgg cccgcatcca cggcgctgcg gtggcttccg
     1861 cgccctgagg c
//
LOCUS       DAOQOC010000653         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43485_length_1870_cov_3.460606, whole genome shotgun sequence.
ACCESSION   DAOQOC010000653 DAOQOC010000000
VERSION     DAOQOC010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43485_length_1870_cov_3.460606"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PL106_10355"
     CDS             <1..236
                     /locus_tag="PL106_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA33510.1"
                     /translation="QQDAGPTVFSPFKRDSDYFDAKQQFMLNLRVQDIDGLLKKLEAA
                     GVRIDPKRQDEDYGRFAWVYDPEGNKIELWEPK"
     gene            295..585
                     /locus_tag="PL106_10360"
     CDS             295..585
                     /locus_tag="PL106_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33511.1"
                     /translation="MRLLYVSITDPSKEKLVTDILHEIEGVEVRRGPIKAERPARRTT
                     KTPTAKRAKAIKPMTPKEKKFVKGLTRAFKDMNDHIAGKKKLKTLDEFLNEL"
     gene            575..904
                     /locus_tag="PL106_10365"
     CDS             575..904
                     /locus_tag="PL106_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33512.1"
                     /translation="MSYRIVLHGNFEREARPLLKRYRSLAVELSVLRDGLKLDPRQGQ
                     PIGNDCYKIRVAIKSKAKGKSGGARVITCVVAVREEVYLLSIYDKSEQSTLTDRRLKE
                     LLKLVPR"
     gene            943..1503
                     /locus_tag="PL106_10370"
     CDS             943..1503
                     /locus_tag="PL106_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNA33513.1"
                     /translation="MRPLRFSINVTLDGCYDHTVGIPDEGLHRNAEAYIAGTDAMLLG
                     RKTYQLMESAWRMPPSGQWPDWMPLWMQPFARTMDAAKKYLVSSTVDTAEWNTVMVRG
                     DLATFVRQLKQQPGKGIGVGGVQLAHALTEMGLIDEYQIVVHPRIAGRGPTLFAGLSK
                     MVDLKLVGREEFSSGAVALRYEVRKP"
     gene            1658..>1870
                     /locus_tag="PL106_10375"
     CDS             1658..>1870
                     /locus_tag="PL106_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33514.1"
                     /translation="MHPLYASTALAAALFASHPAAHAQWQPVGIPAFSTNMVDYPVLA
                     IGTDDLPYVVFSDWAHTSKLTAMKYSG"
BASE COUNT          418 a          546 c          573 g          333 t
ORIGIN      
        1 ggcagcagga tgccgggccc acggtgttct cgcccttcaa gcgcgacagc gactatttcg
       61 atgccaagca gcaattcatg ctgaacctgc gcgtgcagga catcgatggc ctgttgaaga
      121 agctcgaggc cgccggggtg cgcatcgatc ccaagcgaca ggacgaggac tacggccgct
      181 ttgcgtgggt gtacgatccg gaggggaaca agatcgagtt gtgggagccg aagtaatggg
      241 ttgcatccgc tgaaacgcga tgcaccgcac ctagcgccgt atcttagcct catcatgagg
      301 ctgctgtatg tgagcatcac ggatccgagc aaggaaaaac ttgtcacgga catcctgcac
      361 gagatcgagg gcgtggaggt acgacgcggt cctatcaaag cggaacgacc ggcacggcgg
      421 accaccaaaa cgccaacagc caaacgcgcc aaggccatca agcccatgac accgaaggag
      481 aagaagttcg tgaagggatt gacccgcgcg ttcaaggaca tgaacgatca catcgccggg
      541 aaaaaaaagc tgaagaccct ggacgagttc ttgaatgagc tataggatcg tccttcacgg
      601 taacttcgaa cgtgaagcgc gcccattact gaagcgctat cgttcgctcg ctgtggaact
      661 ctccgttctt cgggacggat tgaagttgga tccgcgccaa ggtcaaccca ttggaaacga
      721 ctgctacaag atccgagtgg ccatcaagtc caaagccaag gggaagtccg gcggtgcgcg
      781 tgtgatcaca tgcgtggtgg ccgtgcgcga agaggtgtac ctgctttcca tctatgacaa
      841 gagcgaacag agcacactga cggataggcg cttgaaggag ctcttgaagc tggtgccgcg
      901 ctgatcggcg actttggaac cgcacgagca ctggcctccc ccatgcgccc cctccgcttc
      961 tccatcaacg tcacgttgga cggctgttac gatcacaccg tgggcatccc ggacgagggc
     1021 ctgcaccgca atgcggaagc gtacatcgcc ggtaccgatg ccatgctgct gggccggaag
     1081 acctatcagt tgatggagag cgcctggcgc atgcctccga gcgggcaatg gcccgactgg
     1141 atgccattgt ggatgcagcc cttcgcgagg acgatggacg cggcgaaaaa atacctcgta
     1201 tccagcaccg tggacaccgc ggagtggaat acggtaatgg tgcggggtga cttggccacc
     1261 ttcgttcggc agctcaagca acagccgggc aagggcatcg gcgtgggcgg tgtgcaacta
     1321 gcacatgctt tgaccgagat gggcctgatc gatgagtacc agatcgtggt gcatccgcgc
     1381 atcgccggtc gtgggcccac cttgttcgcg gggctgagca agatggtgga cctgaagctc
     1441 gtgggccgcg aggagttcag ctcgggggcg gtggcgctgc ggtatgaagt gaggaagccg
     1501 tagtgcgcaa acagttccac gcacagctca agcgatcagg ccgggcccac gtgaaaaacc
     1561 tgccggcctt cccggaaaat acggtgatct acggattcgg cgcgccgatg aaacctcttc
     1621 atttgcctgt ccgatacaag gccacaatca tataagcatg caccctctgt acgcctccac
     1681 tgccctggct gccgcactct tcgccagcca tcccgccgcc catgcccagt ggcaacccgt
     1741 aggcatcccc gccttcagca ccaacatggt ggactacccg gtgctggcca tcggcacgga
     1801 tgatctgccg tacgtggtgt tctccgattg ggcacatacc agcaagctca ccgcgatgaa
     1861 gtacagcggt
//
LOCUS       DAOQOC010000654         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43511_length_1869_cov_4.187431, whole genome shotgun sequence.
ACCESSION   DAOQOC010000654 DAOQOC010000000
VERSION     DAOQOC010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43511_length_1869_cov_4.187431"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1254)
                     /locus_tag="PL106_10380"
     CDS             complement(190..1254)
                     /locus_tag="PL106_10380"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNA33515.1"
                     /translation="MRLSAVLPALVLSCMCVAQVSLTGRVQDARTAEPLAFVPVNEAG
                     TREGTLSDIDGRFALSVPALPTHLRFSYVGYRTLEIEVTTAIPLVVRLEQVSTELRQV
                     TVSGTDNPAHRIIRRAWANRRENDGMKYRAHRYVSYGKTVLDPELDSAVVGDTARLAL
                     LDTSTRKVYGLAERQHLLLIESVTKKSFIPPDKAREEVLAMRVSGLKDPSILTLLAST
                     ATFSIYEPEIRISNKTYLGPLGPTATTKYRFNLEDTLYQGDDSVYVISYRPRAGAKFN
                     ALEGLLYINTDGYAVQNVTAGPSEREGTSIRLQQVHARVGGKWFPEQLNTFIYFDNLQ
                     LQASLTDDTTTTKRTTLNIK"
     gene            complement(1352..1729)
                     /locus_tag="PL106_10385"
     CDS             complement(1352..1729)
                     /locus_tag="PL106_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA33516.1"
                     /translation="MEKILIDTSVFIGMARKQPVAEEAMARTRGAEVLFCDIVLAEIL
                     AGAKNQEEYRRTLAHITRAYRILDITPQACVLFREILATKGANDGVHVSDFMIAATAM
                     AHGCPLLTLNRKHFERIKGLVLV"
BASE COUNT          350 a          555 c          566 g          398 t
ORIGIN      
        1 ttgctaaggg tcggcttgtg tgtcgggcaa aaccaaatgt gccattttgt gcggttggtt
       61 aaaaattgaa ttaaaaaaat gtgcggtggg gaaaataaaa aatacagaag ggtaggcaat
      121 gagtgtcggc tcagttgctg tccagttgaa atatggtctg tatgttggtc acctgtcaaa
      181 attagcgttt cattttatgt ttaaagtcgt ccgtttggtc gttgttgtgt cgtctgttag
      241 gcttgcttgt aactgcaggt tgtcgaaata gatgaaggtg ttcagttgct cggggaacca
      301 tttgcctccc acccgtgcat gcacctgctg caggcggatg ctggtgcctt cgcgctcgga
      361 ggggccggcc gtcacgttct gcacggcgta cccgtcggtg ttgatgtaga gcagaccctc
      421 cagcgcgttg aacttcgctc ccgcacgcgg ccggtagctg atcacgtaca cgctgtcgtc
      481 gccctggtac agcgtgtcct ccagattgaa gcggtacttg gtggtggccg ttggacccaa
      541 tggccccagg tatgttttgt tgcttatgcg gatctccggc tcgtagatgc tgaaggtagc
      601 ggtgctcgcc aggagcgtca ggatggaagg atccttgaga ccgctcacgc gcatggccag
      661 cacctcttcg cgggccttgt ccggtggaat gaagctcttc ttggtcacac tctcgatcag
      721 gagcaggtgc tgccgctcgg cgagaccgta caccttgcgc gtcgaggtgt cgagcagggc
      781 cagccgcgcg gtatcaccca ccacggcgct gtccaactcc ggatcgagca cggtcttgcc
      841 gtagctgacg tagcggtgcg ctcggtactt catgccgtcg ttctcccggc ggttcgccca
      901 cgctcttcgg atgatccggt gcgcggggtt gtcggtgccg ctcaccgtta cctggcgcaa
      961 ctcggtgctc acctgctcca agcgtaccac gagcgggatg gccgtggtca cttcgatctc
     1021 caaggtccgg tatcccacat agctgaatcg caagtgcgtc ggcaacgcgg gaacgctgag
     1081 tgcgaaacgt ccgtcgatgt cgctgagcgt gccctcgcgt gtgcccgcct cgttcaccgg
     1141 cacgaacgcc agcggctccg ccgtgcgcgc atcttgcacg cgacccgtga gcgacacctg
     1201 cgcgacacac atgcaggaga ggaccagagc gggcagaaca gcggacaggc gcatgaacaa
     1261 ggggcttctc ctgtgacggg caacggcgaa gaaaggatac agaccgggta cgtgccggca
     1321 cgatccacat accggaaccg cacagcgacc ctcaaacgag caccagcccc ttgatccgct
     1381 cgaagtgctt gcgattcagg gtcaacagcg ggcagccgtg ggccatggcc gtagcggcga
     1441 tcatgaaatc gctgacatgc acaccatcgt tggccccttt ggtggccagg atctcgcgga
     1501 acagcacaca ggcctgcggt gtgatgtcca ggatccggta tgcacgggtg atatgcgcca
     1561 acgtacgcct gtactcctcc tggttcttcg cgcccgccag gatctcggcg agcacgatat
     1621 cgcagaaaag cacctcggca cccctggtgc gggccatcgc ttcttcggcc acaggctgtt
     1681 tccgggccat gccgatgaaa acgctggtgt cgatcaggat cttttccatg ccttctcccg
     1741 gagaccttcc agggtgacat cacggtccgc ccagatgccc cagaattcca tcagaggctc
     1801 ctctttgcgc gccaactccc gctcgatgaa ctcggaaacg agcgccgaga tgctccgctt
     1861 ccgcctgcg
//
LOCUS       DAOQOC010000655         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43557_length_1868_cov_4.323773, whole genome shotgun sequence.
ACCESSION   DAOQOC010000655 DAOQOC010000000
VERSION     DAOQOC010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43557_length_1868_cov_4.323773"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PL106_10390"
     CDS             <1..587
                     /locus_tag="PL106_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNA33517.1"
                     /translation="MNPNDPSQQLVLAFETAKEWDTWLAKHHATSTGVYIRVGKKGSG
                     IRSITCPEALEVALCYGWIDAIRKSHDANTFLQRYCPRGPRSIWSEINKKKVQQLIKA
                     RLMLPAGLAAVKRAKENGQWDKAYAPASSITVPPDLEAALKKNNKARDFFATLKGSNR
                     YAFLHRLHTAKKAETRARRLEQFIGMLERGETFY"
     gene            599..1033
                     /locus_tag="PL106_10395"
     CDS             599..1033
                     /locus_tag="PL106_10395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNA33518.1"
                     /translation="MNDREVRTTRIIAAPCERVFAAWTDPAQLASWWGPQGFTNTFHR
                     FELKPEGMWEFTMHGPNGMDFHNTCVFKRIEPPGYLEFDHLKEMHFYKAIVNFTGVEG
                     GTRVDWIMRFNTIEELAPIRGFIEKANEENLDRMERQLTKKP"
     gene            1040..1564
                     /locus_tag="PL106_10400"
     CDS             1040..1564
                     /locus_tag="PL106_10400"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA33519.1"
                     /translation="MPYVLLILAVLIVGILIVASTKPNTVHYERSTVINAPPEKIVPH
                     LVDFHKWEAWSPWEKLEPGMKRSYSGAASGVGATYAWEGKKKAGAGTMEILETGISGV
                     KIDLRFIKPWKAECIAQFHFTPQPNGTDVRWSMDGPNTFMGKIFGLFMNMDKLVGKDF
                     EIGLAGLKTVVEKD"
     gene            1589..>1868
                     /locus_tag="PL106_10405"
     CDS             1589..>1868
                     /locus_tag="PL106_10405"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNA33520.1"
                     /translation="METHSAQRTRNNVSTPALDELRITRVFNAPRKLVWQAWTTPAML
                     VHWFGCAAFSTIDAEADVCEGGQWRVVMRAPDGTDYPAYGTYTAVRPID"
BASE COUNT          424 a          581 c          550 g          313 t
ORIGIN      
        1 gaatgaaccc caacgacccc tcccagcaac tcgtccttgc cttcgagacc gccaaggaat
       61 gggacacctg gctggcgaag caccacgcga cctccacggg cgtgtacatc cgcgtgggca
      121 agaagggcag cggcatccgc tccatcacct gccccgaagc cctggaagtg gccctgtgct
      181 acggctggat cgatgccatc cggaaaagcc atgatgccaa cacctttcta cagcgctatt
      241 gcccgcgcgg cccgcgcagc atctggagcg agatcaataa gaagaaggtg cagcagctca
      301 tcaaagccag gctgatgcta ccagcgggtc tcgccgccgt gaagcgcgca aaggaaaatg
      361 ggcaatggga caaggcttat gcgccggcca gcagcatcac cgtgccgccg gacctggaag
      421 ccgcgctgaa gaagaacaac aaggcccgcg acttcttcgc cacactgaaa ggcagcaacc
      481 gctatgcatt tctccatcgc ctgcacacgg ccaagaaggc cgaaacgcgc gcgcggaggt
      541 tggagcagtt catcggcatg ttggaacgcg gcgaaacgtt ctactgaaca acaatgccat
      601 gaacgaccgt gaagtacgca ccacccggat cattgccgcc ccatgcgagc gggtctttgc
      661 ggcatggacc gatcccgcac agctcgcgag ctggtgggga ccgcaaggct tcaccaacac
      721 cttccaccgt ttcgagctga agccggaggg tatgtgggag ttcaccatgc acggcccgaa
      781 cggcatggac ttccacaaca cctgcgtatt caagcgtatc gaaccgcccg gctacctgga
      841 gttcgaccac ttgaaggaaa tgcacttcta caaggcgatc gtgaacttca ccggagtgga
      901 aggcggcacg cgcgtcgatt ggatcatgcg cttcaacacc attgaggaac tggcgccgat
      961 ccgcgggttc attgaaaagg cgaatgaaga gaacctcgat cggatggaac ggcaattgac
     1021 caagaaaccc tgaaacccca tgccttacgt actcctgatc cttgccgtcc tcatcgtcgg
     1081 catcctcatc gtggcttcca cgaaacccaa caccgtccac tacgaacgca gtacagtgat
     1141 caatgctccg ccggagaaga tcgttccgca cctcgtcgat ttccacaaat gggaagcctg
     1201 gagcccgtgg gagaagttgg agccgggcat gaagcggagc tattctggcg cggccagcgg
     1261 cgtgggggct acctacgcct gggaagggaa aaagaaggcc ggtgcgggca ccatggagat
     1321 cttggagacg ggcatcagcg gcgtgaagat cgacctgcgc ttcatcaagc cgtggaaggc
     1381 tgaatgcatc gcgcagttcc atttcacacc gcagcccaac ggcacagacg tgcgctggtc
     1441 gatggacggt cccaacacct ttatgggcaa gatcttcggc ttgttcatga acatggacaa
     1501 gctggtgggc aaggatttcg agatcgggct ggcggggttg aagacggtgg tggagaagga
     1561 ttgatcggcc tccggtacct tcgcgttcat ggaaactcac agcgcccaac gcacacgcaa
     1621 caacgtttcc acccccgccc ttgatgaact ccgcatcacc cgcgtgttca acgcgccgcg
     1681 caagcttgtg tggcaggcat ggaccacccc ggccatgctc gtgcattggt tcggctgcgc
     1741 ggccttcagc accatcgatg ccgaggccga tgtgtgcgag ggcggccaat ggcgcgtggt
     1801 gatgcgcgcc ccggacggca cggactaccc ggcttacggc acctacaccg ccgtgcgccc
     1861 catcgatc
//
LOCUS       DAOQOC010000656         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43586_length_1868_cov_2.293988, whole genome shotgun sequence.
ACCESSION   DAOQOC010000656 DAOQOC010000000
VERSION     DAOQOC010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43586_length_1868_cov_2.293988"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PL106_10410"
     CDS             <1..399
                     /locus_tag="PL106_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA33521.1"
                     /translation="LALEVDDAVPAKLIGDPTRLNQIVMNLAGNAVKFTERGGVTIRA
                     SIAERSIDRCSLVVAVSDTGIGIPEDRLEKIFEEFTQAYSDTTRKYGGTGLGLTISKR
                     LAELQGGSISVTSQRDKGSTFTVAIPYGLG"
     gene            680..1693
                     /locus_tag="PL106_10415"
     CDS             680..1693
                     /locus_tag="PL106_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33522.1"
                     /translation="MRTSPTLVAFLGILPCATLCGQVTLSSGTADPSAGDALTLSTCS
                     WVDPGPAGTNITWAQPDLEVLSTNVSNYVSVASTGLAGTYPNATVAYDGGGGTYLFYR
                     EAATGYEQDGFVLNMYSATCSDRLLLLPYPLSYGNTWSDNATCTVTDGSTSWARTMSV
                     TGTADGWGSIILPAGTVTNVLRVHWVENMVDNQYDPPSTTVFDYYSWYRPETPGPVFS
                     TSNIDVTIFGFSTSDSSSTLLDLSAIGMQEVLDHAIGVRVMPNPVPDRAEVVFGLGAG
                     ERATIELLDVSGRVVRTSTRAIQVSGAQREFIDMVALPAGAYVVRITDGRGAYGTSRV
                     VKR"
BASE COUNT          356 a          584 c          559 g          369 t
ORIGIN      
        1 ctcgcactgg aggtcgatga tgcggtccct gcgaagctga tcggtgatcc gacccgattg
       61 aaccagatcg tgatgaacct tgccggcaac gccgtgaaat tcacggagcg gggaggtgtg
      121 accatccgtg cctccatcgc ggagcggtcc atcgatcgct gttcgctcgt tgtcgcggtc
      181 agcgacacgg gcatcggcat tccggaggac cggctggaaa agatattcga ggagttcacg
      241 caggcataca gcgacaccac acggaagtat ggtggtaccg gcttggggct caccatcagc
      301 aaacggctcg ctgaattgca gggaggaagc atatcagtga ccagccagcg cgacaaaggc
      361 agcacgttca cggtggcgat cccctatggc ctggggtgat accgtgcgcc aggaccacat
      421 tcaacccgat ggttactgag acggccagaa agaaccggtc ggtcccgggt tcaagggtgc
      481 gagggtgcaa gagtcacgac tctcgcaccc tcgcactttc gtaccccatg gttcattctg
      541 atcacgatgg tcgagagacc ggatcttcat caccggctca gtattactgt tcggaacatc
      601 cgatcgtgga tggatccttt ggtcgccgca tatacggctt cggggatggg cgtagttttc
      661 tcaccacaag aacaaccaca tgagaacaag ccctaccctt gtagcctttc ttggcatcct
      721 gccatgtgca accctctgcg gccaggtcac cctcagcagc ggcacggcgg atccctccgc
      781 cggcgatgcg ctcaccctgt ccacatgctc ctgggtggat cccgggcccg ccggcacgaa
      841 catcacctgg gcccagcccg acctggaggt gctctccacc aacgtttcca actacgtctc
      901 ggtggcctcc acggggctgg cgggaaccta tcccaatgca acggtagcgt atgatggggg
      961 gggaggcacg tacctcttct atcgggaggc cgccaccggc tacgagcagg acggtttcgt
     1021 cctgaacatg tattcggcca cgtgttcgga ccggctgctc ctgcttcctt atcccctgag
     1081 ctacggcaac acctggtcgg acaatgcgac atgcaccgtg accgacggat ccacgtcctg
     1141 ggcgcgcacc atgagcgtga ccggtacagc cgatggatgg gggagcatca tccttcccgc
     1201 tggcacggtg acgaacgtgc tgcgtgtgca ttgggtggag aacatggtgg acaaccaata
     1261 tgatccgcct tccaccaccg tcttcgacta ctattcctgg tatcgtcctg agacgcccgg
     1321 tcctgtcttt tccacctcca acatcgatgt gaccatcttc ggcttctcta ccagtgattc
     1381 gagctccacg ctgttggatc tgagcgcgat cggcatgcag gaggtgctcg accatgcgat
     1441 cggggtgcgg gtgatgccca acccggttcc ggatcgtgcg gaggtggtct tcggcctcgg
     1501 tgcgggagag cgtgcgacca tcgagttgct cgacgtttcc ggtcgcgttg tccgcaccag
     1561 cacacgggcc atccaggtgt ccggtgcgca gcgtgagttt atcgacatgg tcgcgcttcc
     1621 ggccggggca tacgtggtgc ggatcaccga tggccgtggt gcctatggaa caagtcgcgt
     1681 ggtgaagcgc tgagcgcccg gcccccttga aacaggagtt tgaccgcaca ggtgcgcggt
     1741 cgcgcgggat cctgcacgca gtgatccgaa accggggtgg gcagaattcc gcatcttcac
     1801 cacccctctt tacacccatg gcattcccca ccgacctcga gatcgcccaa aaggccaccc
     1861 tgcaaccc
//
LOCUS       DAOQOC010000657         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43607_length_1867_cov_3.513245, whole genome shotgun sequence.
ACCESSION   DAOQOC010000657 DAOQOC010000000
VERSION     DAOQOC010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43607_length_1867_cov_3.513245"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="cas5c"
                     /locus_tag="PL106_10420"
     CDS             complement(<1..549)
                     /gene="cas5c"
                     /locus_tag="PL106_10420"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HNA33523.1"
                     /translation="MKNIIEFKVWGRYALFTDPLTRVGGEKCSYHIPTYEALKGVVKS
                     IYWKPTIIWVIDEVRVMKRIRTQTKGMKPVDMSGGNSLAYYTYLADVEYEVRAHFEPN
                     KHRPELMKEDYIEGKHFNIAKRMLDRGGRQDIFLGTRECQGYVEPCTYEDIDKGHYGD
                     AGEVGYGLTFHGFSADVRDLLDS"
     gene            complement(546..>1867)
                     /locus_tag="PL106_10425"
     CDS             complement(546..>1867)
                     /locus_tag="PL106_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33524.1"
                     /translation="DVRRLHRLSKAIIVFDEVQTLPIKTFHLFCNAMNYIVEHCESTV
                     LLCTATQPLLHELDEKKGRLRLGAYNNRSDKDYELMPDVKKLFTDLERVDVLDMRKPN
                     GWTTAEVAELARTCMQDHGSCLVVVNTKAMALALYEQCGGASNNDLFHLSTHMCPAHR
                     KAVLATIRTRLEPGKEKPTLCISTQLIEAGVDVDFGSAIRSHAGLDSIGQASGRCNRN
                     GRHAKRPVYVVNPTDEKVDMLEEIRVGQDDFARFMDDDFKKAPQDFDNNPLSDAAMKR
                     YFTYFFFDRRAAMSYPVGVPTFDRGDSLLNMLAENGMALDAHNRSHDKPYDGLPQSFM
                     SAAKVFRAIDSPTQGVIVPYGDEGSKVISELCAEYDPTKAYELLRRAQQYSVNVFPNV
                     LRPLRDANAVREVQEGTGILFLNDRYYEKNTGLSEVPINPMKPLITP"
BASE COUNT          371 a          520 c          528 g          448 t
ORIGIN      
        1 ggagtcgagc aggtcccgca cgtccgcgga gaaaccgtgg aaggtcaggc cgtaaccgac
       61 ctcaccagca tcgccatagt ggcccttgtc gatgtcttcg tatgtgcatg gctccacata
      121 gccttggcat tcacgggtgc caaggaaaat gtcctgccga ccaccgcgat ccagcatgcg
      181 cttagcgatg ttgaagtgct tcccttcaat gtaatcctcc ttcatcaatt ccggtcggtg
      241 cttgttcggc tcaaagtgtg cccgaacttc atactcaaca tcagcgaggt aggtatagta
      301 ggccaacgaa ttgcctccgc tcatgtcaac gggcttcatt cccttggtct gggtgcgtat
      361 ccgtttcatc actcgcactt catcaatgac ccagatgatg gtgggcttcc agtagatgga
      421 cttcaccacg cctttcaagg cctcgtaggt gggaatgtgg taggagcact tctcgccacc
      481 cacacgggta agcggatcgg tgaagagcgc atagcgcccc cacaccttga actcaatgat
      541 gttcttcatg gggttatcag tggcttcatt gggttgatag ggacttcgct gagcccggtg
      601 ttcttctcgt agtaccggtc gttcaaaaac agaatgcccg tgccttcctg aacctccctc
      661 accgcattcg catcccggag ggggcgcaac acgttcggga acacgttcac ggagtactgt
      721 tgcgcacggc gcaacaactc gtaggccttt gtcgggtcgt attcagcgca gagttcgctg
      781 atcactttgg aaccttcatc gccgtagggc acaatgacac cctgagtggg gctgtcgatg
      841 gctcggaaga ctttggcagc gctcatgaag gattgaggaa gtccatcgta tggtttgtcg
      901 tgactacgat tatgcgcatc aagagccatt ccattctctg cgagcatgtt caatagtgag
      961 tcgccacggt cgaatgttgg tactccgact ggataagaca tcgctgcccg tctatcgaag
     1021 aagaagtagg tgaagtagcg cttcatggcc gcatcgctga gcggattgtt gtcgaagtcc
     1081 tgtggtgcct tcttgaagtc atcgtccatg aagcgcgcga agtcgtcttg tccgaccctg
     1141 atctcttcga gcatatccac tttctcatcg gtggggttca ccacatagac cggtcgcttg
     1201 gcatggcgac cattgcggtt gcagcgcccg gacgcttgcc cgatggagtc caggcccgca
     1261 tgggaacgga tggcactgcc gaagtccaca tccaccccgg cttcgatgag ttgggtgctg
     1321 atgcagagcg tgggcttttc cttgccgggt tccagccggg tgcgtatggt ggcgagtacc
     1381 gccttgcgat gggccggaca catgtgtgtg cttaagtgga aaaggtcgtt gttggacgcc
     1441 ccgccgcatt gctcataaag cgcaagggcc atggccttgg tgttcaccac cacgaggcaa
     1501 cttccatggt cctgcatgca cgtccgagca agctccgcga cttccgccgt ggtccatcca
     1561 ttgggcttac gcatgtctaa cacatccacc cgttcaaggt cggtgaaaag cttcttcaca
     1621 tcgggcatca gttcatagtc cttgtctgac cggttgttat aggctcccag ccgtaagcgg
     1681 cctttcttct catccagttc atgcaacagc ggttgggtgg cggtgcagag gagcacggtg
     1741 ctttcacaat gttccacgat gtaattcatc gcgttgcaga acaggtggaa ggtcttgatg
     1801 gggagcgtct gcacctcgtc gaacacgatg atcgctttgg acaggcggtg caggcgccgc
     1861 acatcgc
//
LOCUS       DAOQOC010000658         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43802_length_1863_cov_4.170354, whole genome shotgun sequence.
ACCESSION   DAOQOC010000658 DAOQOC010000000
VERSION     DAOQOC010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43802_length_1863_cov_4.170354"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /gene="ftsA"
                     /locus_tag="PL106_10430"
     CDS             <1..953
                     /gene="ftsA"
                     /locus_tag="PL106_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNA33525.1"
                     /translation="HKLVMPPGEEIIHVIPQEYIVDHEQGIKDPIGMSGVRMEADFHV
                     ISGQLTAARNINKCVHRAGLEVTELILEPLASADAVLSDEEKEAGVVLVDIGGGTTDI
                     AIFSDHIIRHTAVIPYGGNVITDDIKMGCSILRDQAETLKTRFGSALAAENKENEVVC
                     IPGLRGREPKEISLKNLAHIIQCRMEEILEFVHFEIRNSGLEKQLSAGIVITGGGAQL
                     KHLRQLTEYVTGMSVRIGYPNEHLAKSNVDAVTSPLYATGVGLVMMGLDHMDRSRLRT
                     ASAVPSTGPKVKGHSQPSRVGNFFERVLKGATDLLDDDES"
     gene            1101..>1863
                     /gene="ftsZ"
                     /locus_tag="PL106_10435"
     CDS             1101..>1863
                     /gene="ftsZ"
                     /locus_tag="PL106_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNA33526.1"
                     /translation="MKFQLPTELSSIIKVIGVGGGGSNAVNHMHAQGIPGVDFIICNT
                     DKQALDMSSIPVKLQIGAAHTEGLGAGAKPERGALAAQENIEDIRQLLSSRTKMIFIT
                     AGMGGGTGTGAAPVIARVARDMNILTVGIVTMPFEWEGQRRKRQATAGVEELRQAVDT
                     LLVINNDRLRDLYGNLEVDEAFAHADNVLSTAARGIADIIYTTGKVNVDFEDVRTVMS
                     DSGVAIMGMAEAEGENRAIRAAQEALNSPMLNDSDI"
BASE COUNT          412 a          599 c          572 g          280 t
ORIGIN      
        1 tgcacaagct ggtgatgccg ccgggcgaag agatcatcca tgtgatcccc caggagtaca
       61 tcgtggacca tgagcagggc atcaaggatc cgatcggcat gagcggtgtg cgcatggagg
      121 ccgacttcca tgtgatcagt ggccaactca cggctgcccg caacatcaac aagtgcgtgc
      181 atcgtgcggg gctggaggtg accgagctga tcctggaacc gctggccagc gccgatgcgg
      241 tactgagcga cgaggagaaa gaagcgggcg tggtcctcgt ggacatcggc ggcggcacca
      301 ccgacatcgc cattttcagc gatcacatca tccgccacac cgcggtgatc ccgtacgggg
      361 gcaacgtgat caccgacgac atcaagatgg gctgctccat cctgcgcgat caggccgaaa
      421 ccctcaagac ccgcttcggc agcgcactgg ccgccgagaa caaggagaac gaagtggtct
      481 gcatccccgg actgagagga cgggaaccca aggagatcag cctgaagaac ctcgcgcaca
      541 tcatccagtg ccgcatggag gagatcctgg aattcgtcca tttcgagatc cgcaacagcg
      601 ggctcgaaaa gcagctcagc gcgggcatcg tgatcaccgg tggtggagcg caactcaagc
      661 acctgcggca gctcaccgaa tacgtcacgg gcatgagtgt ccgcatcggt tatcccaacg
      721 agcacctggc caagagcaac gtggatgccg tgaccagtcc gctctacgcc acgggtgtcg
      781 ggctggtgat gatgggactg gaccacatgg accgcagtcg gttgaggacc gcctcggccg
      841 tcccctccac cggacccaag gtgaagggac acagccagcc cagccgggtc ggcaacttct
      901 tcgaacgcgt actcaaaggc gccactgatc tcctggatga cgacgaatcc tgaacggatc
      961 atgcagcgaa cagcggacca tcgatcatga aatgagccgt tcgcgcgacc tgcctgaagc
     1021 ccgaccccaa ccaacccgcc caagccgtga accccgaatc ccctcgacca acgcctccgc
     1081 cgggcctcgg cgccaccagc atgaaattcc aactacccac tgaattgtcc agcatcatca
     1141 aggtgatcgg cgtgggcggt ggtggcagca atgccgtgaa ccacatgcac gcccaaggca
     1201 tccccggcgt ggacttcatc atctgcaaca ccgacaagca ggccctggac atgagctcga
     1261 tcccggtgaa gctgcagatc ggtgctgcgc acacggaagg ccttggtgcg ggtgccaaac
     1321 ccgaacgtgg cgcccttgca gcgcaggaga acatcgagga tatccggcag ctgctgagct
     1381 cccgcaccaa gatgatcttc atcaccgcgg gcatgggggg cggtacgggt acaggcgctg
     1441 ctcccgtgat cgcccgcgtg gcgcgcgata tgaatatcct caccgtgggt atcgtcacca
     1501 tgcccttcga gtgggagggg cagcgccgca agcggcaagc gaccgccggg gtcgaagaat
     1561 tgcgccaggc cgtggacacg ctgctggtga tcaacaacga ccggctgcgc gacctgtacg
     1621 gcaacctgga ggtggacgaa gcattcgccc acgccgacaa cgtcctctcc accgccgcgc
     1681 gcggcatcgc cgacatcatc tacaccaccg ggaaggtcaa cgttgacttc gaggatgtgc
     1741 gcacggtgat gagcgatagc ggggtggcca tcatgggcat ggccgaggcc gaaggtgaga
     1801 accgtgccat ccgcgcggcc caggaagccc tcaactcgcc catgctcaac gacagtgaca
     1861 tcc
//
LOCUS       DAOQOC010000659         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_43953_length_1860_cov_3.015512, whole genome shotgun sequence.
ACCESSION   DAOQOC010000659 DAOQOC010000000
VERSION     DAOQOC010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43953_length_1860_cov_3.015512"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1860
                     /locus_tag="PL106_10440"
     CDS             23..>1860
                     /locus_tag="PL106_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA33527.1"
                     /translation="MEVPEDAPATAERFSEGKGPGEKITSIVHLRSGALVFLDAAQGL
                     RQWDPTAGAFTPFPVKELADQQRATSLFEDGQGALWLGTQDHGALRWKDGRTTTYDQP
                     EGMPGSFIYGFGEDAHGQVWVSTWGHGVVRIEKDGPRLFGISNGLHGRNVRAIARDRE
                     GNMLFATNDEGLDIFKGDRFLTFGEDDGLVEPHVRAVAVTPDGRIWFGTQGGVTVLRP
                     GGTGAMNVATLSAQSGDLTDNNVRALLADPSGTVWIGTEDGGLLELGPDASHPVPHDE
                     ISALLGNNKVTALAPSSRGGLYVGTIGGLIHLMPGKVPTMLGMQEGLSGTDITALFTD
                     AQGNTWAGTASSGISRIPAAGEERAVNVDVGAVISPTSFAQDKEGRIWTGTLSQGVIV
                     IKDDKVDVRYGQDDGLLSNNVRSLASDAEGTVWVGTNLGLNRLRRTGTAKSGPGTNDR
                     VFMSFTGRGGFTGIEALAGAAVRTDDGYLWFGTANGAVRVTTGHWLDQAEPPLIAVRV
                     LKVNLLERDAIDGVDLSHTESDIRIEYGCVSLIDQGAVRYRYMLAGLQEDWQPVTSET
                     SAHYPGLSPGRYVFKVCARNRAGIWSEPVLYHFTVLPPWYRSWWFYG"
BASE COUNT          331 a          565 c          649 g          315 t
ORIGIN      
        1 cggcgacggt gcccgccgga tcatggaggt tcctgaggat gcaccggcca cggccgaacg
       61 gttcagcgag ggcaagggtc ccggagaaaa gatcaccagc atcgtgcatc tccgttccgg
      121 agcgctcgtc tttctggatg ccgcacaggg cttgcgccag tgggatccga cagcgggtgc
      181 gttcacaccg ttccccgtga aggaactggc cgatcagcag cgggccacca gcctcttcga
      241 ggacggccag ggagcgctct ggctgggtac ccaggatcac ggagctttgc gctggaagga
      301 tggccggacc accacgtacg atcagcccga aggcatgccc ggcagcttca tctacgggtt
      361 cggggaagat gcccatggac aggtctgggt gagcacctgg ggtcatggtg tggtgcgcat
      421 cgagaaggac ggtccgcggc tgttcggcat cagcaacggc ctgcacgggc gcaatgtccg
      481 cgcgatcgca cgggaccgag aaggcaacat gctcttcgcc accaacgacg aggggctgga
      541 cattttcaag ggcgatcgtt tcctcacctt cggcgaggac gatggcctgg tggagcccca
      601 tgtgcgggcc gtggccgtga ctccggatgg acggatctgg ttcggtaccc agggcggcgt
      661 caccgtgctg cgaccaggag gcactggagc catgaacgtg gccacgctct cggcgcagag
      721 cggcgacctc accgacaaca atgtgcgcgc cctgctggct gatccttcgg gaacggtatg
      781 gatcggtacg gaggatggag gtctgctgga gttgggacct gatgcttcgc atcccgtgcc
      841 gcatgatgag atcagtgcgc tgctgggcaa caacaaggtg accgcgttgg caccctcgtc
      901 ccggggcgga ctgtacgtgg gcaccatcgg tggcctcatc cacctgatgc cggggaaagt
      961 gccgaccatg ctgggtatgc aggagggcct ctccggcacc gacatcaccg cgctctttac
     1021 cgatgcccag ggcaacacct gggcgggtac ggccagcagc gggatcagca ggatacctgc
     1081 tgccggagag gaacgggccg tcaatgtgga tgtgggggcc gtcatttccc ccacgagctt
     1141 cgcgcaggac aaggaaggcc gcatctggac cggaaccctc agtcagggcg tcatcgtgat
     1201 caaggacgac aaggtggatg tccggtacgg acaggatgac gggctgctct ccaacaacgt
     1261 gcgttcgctg gccagcgatg ccgagggcac cgtgtgggtc ggcaccaacc tcggcctcaa
     1321 tcggttgcgc aggaccggca ctgcgaagtc gggtccgggg acgaacgacc gggtcttcat
     1381 gagcttcacc ggacggggcg ggttcaccgg tatcgaggcg ctcgccgggg cggcggtccg
     1441 cacggacgat ggatacctgt ggttcggtac agcgaacggg gcggtgcggg tgaccaccgg
     1501 ccattggctc gaccaggccg agccgcccct gatcgccgtg cgcgtgctga aagtgaacct
     1561 gctcgagcgc gatgccatcg atggggtgga cctctcgcac accgaaagcg atatccggat
     1621 cgagtacggt tgcgtcagcc tgatcgatca gggtgccgtg cgctaccgct acatgcttgc
     1681 cggcctgcag gaggactggc aaccggtgac gtccgagacc agcgcccact atccgggcct
     1741 gtcgccgggg cggtatgttt tcaaggtctg cgcccgcaat cgcgcgggca tttggagcga
     1801 accggtcctc tatcacttca ccgtgctgcc gccctggtac cggtcatggt ggttctacgg
//
LOCUS       DAOQOC010000660         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44010_length_1859_cov_2.907428, whole genome shotgun sequence.
ACCESSION   DAOQOC010000660 DAOQOC010000000
VERSION     DAOQOC010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44010_length_1859_cov_2.907428"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..465)
                     /locus_tag="PL106_10445"
     CDS             complement(97..465)
                     /locus_tag="PL106_10445"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNA33528.1"
                     /translation="MADHLRTGAAGEQLACQWLERQGFVVLHRNWRHGHDEIDVVARH
                     GRFLVVVEVKTLSSDRWADPEHAVDQRKQARLLRAADALLHTTEEDLELRFDVIAITH
                     QPNGPEILHIPDAFYPTLHE"
     gene            571..1212
                     /locus_tag="PL106_10450"
     CDS             571..1212
                     /locus_tag="PL106_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNA33529.1"
                     /translation="MNNVARSICALLLLLGTGTMATAQSFEGTIEFTKTTGPVVTNYK
                     YFVKGEHIRIEEINARGDAQGIMLVDTRDRTVTALSPDRKLYLDVPNMRLPKEVNVEV
                     KKTGEIKELAGYKCEKWVVKSASEDRNITYWVAADKFDFFMPMLETLNRKDEQAVFFL
                     EIPDAPGVFPMLGVEQKLDGSEVSKLQVTRIAKSVQKPALFEIPADFNKFERN"
     gene            complement(1209..>1859)
                     /locus_tag="PL106_10455"
     CDS             complement(1209..>1859)
                     /locus_tag="PL106_10455"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNA33530.1"
                     /translation="IGFSDVTVLHNALTALGVASLHGQMPFAIGAKSEETREGLRTVL
                     SGKGEWRIAHGEWNEAPFTNDHSHIERLGQCEGILTGGNLSILYSLRGTRYDIDPRGR
                     ILFLEDLDELRYHADRMVQNLKYSGWFKDLAGLIIGGMSDMRDKDPDDPFGMEVEDMI
                     AEVTAPYDYPVCYGFPAGHIPDNRPLVMGAKAKLSVTADGTTLSFEGIGAPGPSAT"
BASE COUNT          360 a          565 c          572 g          362 t
ORIGIN      
        1 ggcccacggc tgccgaagct ctttttcggt cctctggaac cggacggctt gcccgggccg
       61 ccgggtttcc ggccacgggg ccctcgatca ttgcgttcac tcatggagcg tggggtagaa
      121 ggcgtccggg atgtgcagga tctccggtcc gttgggttgg tgggtgatgg cgatcacatc
      181 gaagcgcaat tccaggtctt cctcggtggt atggagcaag gcgtcggcgg cgcgcaacag
      241 tctggcttgt ttgcgctggt ccaccgcatg ctcagggtcg gcccagcgat cactgctgag
      301 ggttttcacc tccaccacca ccaggaaacg gccgtggcgt gccacaacat cgatctcgtc
      361 gtggccgtgc cgccaattgc ggtggagcac cacgaaacct tggcgttcga gccattggca
      421 tgccagttgt tccccggccg ccccggtgcg caggtggtcc gccatcggtt aagaaacccg
      481 gccttgtgtg taacctgagg ggccacatcg ccgttccggg gccgaatttg aggatctttc
      541 ctccgttttc cgaccagaac gaccccgatc atgaacaacg ttgcacgttc catctgcgcc
      601 ttgttgctgc ttcttggcac cggcaccatg gccacggccc aatcctttga gggcaccatc
      661 gagttcacca aaaccaccgg accggtggtc acgaactaca agtacttcgt gaagggtgag
      721 cacatccgca tcgaggagat caacgcccgg ggcgatgccc aggggatcat gctggtggac
      781 acgcgcgacc ggacggtgac cgctttgagc ccggaccgca agctgtacct ggacgttccg
      841 aacatgcgcc tgcccaaaga ggtgaatgtg gaggtgaaga agaccgggga gatcaaggaa
      901 ctggccggtt acaagtgcga gaagtgggtg gtgaagagcg ccagcgagga ccgcaacatc
      961 acgtactggg tggccgcgga caagttcgat ttcttcatgc ccatgctgga gaccctgaat
     1021 cgtaaggacg agcaggcggt gttcttcctg gagatccccg atgccccagg agttttcccc
     1081 atgctgggtg tggaacagaa gctcgacggc agcgaggtga gcaaactgca ggtcacccgg
     1141 atcgccaaga gcgtgcagaa acccgcgctg ttcgagatcc ccgccgactt caacaaattc
     1201 gagcggaact aggtcgcgga cggacctgga gcaccgatcc cctcgaagct cagcgttgta
     1261 ccgtccgcgg taacgctgag ctttgctttt gcacccatga ccagcggtcg attgtcgggg
     1321 atatgccccg ccgggaaccc gtaacaaacg gggtaatcgt acggtgctgt cacctcggcg
     1381 atcatatcct ccacctccat gccgaagggg tcgtcaggat ccttgtcgcg catgtcgctc
     1441 atgcctccga tgatgagccc ggccaggtcc ttgaaccaac cgctgtactt gaggttctgc
     1501 accatgcgat cggcatggta gcgcaactcg tccagatcct ccagaaaaag gatccttccg
     1561 cgcggatcga tatcatagcg ggtgccgcgc agcgagtaca ggatcgaaag gttgccaccg
     1621 gtcaggatgc cctcgcactg gcccaggcgt tcgatgtgtg aatggtcatt cgtgaacggc
     1681 gcctcgttcc attcgccatg cgcgatccgc cattcgccct ttcctgacag gacggtacgc
     1741 aagccttccc gggtttcctc ggacttcgcg ccgatcgcga agggcatttg cccgtggagc
     1801 gaggccacac caagagcggt gagcgcattg tgcagcacgg tcacatcgct gaagccgat
//
LOCUS       DAOQOC010000661         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44070_length_1858_cov_3.002773, whole genome shotgun sequence.
ACCESSION   DAOQOC010000661 DAOQOC010000000
VERSION     DAOQOC010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44070_length_1858_cov_3.002773"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..910
                     /locus_tag="PL106_10460"
     CDS             146..910
                     /locus_tag="PL106_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA33531.1"
                     /translation="MNNGDGLVLPVVVATLLLLFMCGFVVLLLVMNNTRRIRHRAQLA
                     EMRQQLDRAVMDAGRESMYHTLQEIGRELHDNVGQLLSVAQLGLNTTIDSGMVGPALT
                     TARDALEQGTEEVRRMGHDLNVDLWHQRSLVEAIGLEAQRVERVSRIQVDLTVSDTPP
                     VLDPDTSTVLFRVFQVILVNALKHSGATRIGIGISTAKGIGITITDNGRGFDPDQVKA
                     HAGMMNIHQRCALIRWKAECTTSPGNGCTWHLRPTI"
     gene            917..1600
                     /locus_tag="PL106_10465"
     CDS             917..1600
                     /locus_tag="PL106_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA33532.1"
                     /translation="MEHLVALADDHHLMRAGLVNTVNGLGGYRVTLEAGNGQELVSQL
                     ERLDLDGPDAPALAIVDLSMPVMDGYDTIAWLRGHAPRVLPLALTFDGNDEVMMRAVH
                     AGARGFILKSARPAMLKNALDSLILSGWYNSEELVDVRLRNSERRRHEEQERQVVVER
                     ISPREMEFLRLVCDEAEFTYEQIASRMGIQRRSVDNFRIGLFEKFNIKSKTGLVLFAM
                     RWGLINGKS"
BASE COUNT          390 a          528 c          570 g          370 t
ORIGIN      
        1 gtggtgggca tgctccgctt tttgttccag gacagccagg gcgaacaccg tttcctgtac
       61 gagatcatca ccatttcggc catgctacgg tatctgatgg ccgccatttc gtgccatctt
      121 gccgttagat accgtggatg gtccgatgaa caacggtgac ggcctcgttc ttccggtggt
      181 ggtggccacc ttgctgctgc tcttcatgtg cggtttcgtg gtgctgcttc tggtgatgaa
      241 caatacgcgt cggatccgtc accgcgccca actggccgag atgcgccagc aactggaccg
      301 tgccgtgatg gatgccgggc gcgaatccat gtatcacacc ttacaggaga tcggccggga
      361 acttcacgac aatgtgggac aactgctttc cgtggcccaa ctgggtttga acaccacgat
      421 cgacagcggc atggtcggtc ccgcacttac caccgcacgt gatgctttgg aacaaggtac
      481 ggaagaggtg cgtcgcatgg ggcacgacct caacgtggac ctgtggcatc aacgatccct
      541 tgtggaagcg atcggtttag aggctcaacg cgtggagcgt gtttcgcgca ttcaggtgga
      601 tctgaccgtg tcggatacgc cacccgtcct ggatccggat acgagcaccg tgctgttccg
      661 cgtgttccag gtgatccttg tgaacgcact caaacatagc ggtgctaccc gcatcgggat
      721 cgggatcagt accgcaaagg ggatcgggat cacgatcacc gataatggac gtggcttcga
      781 tcctgatcag gtgaaggccc acgcagggat gatgaacatc catcaacgct gtgccctcat
      841 ccgatggaag gcggaatgca ccacctcgcc gggaaacgga tgcacgtggc atcttcgacc
      901 cacgatctga gcatccatgg aacacctcgt cgctcttgcg gatgaccatc accttatgcg
      961 tgctggactg gtcaataccg tgaatgggct gggcggctac agggtgacgc ttgaagccgg
     1021 gaacggtcag gaactggtga gccagctcga acgcctggac ctggacggac cggatgcacc
     1081 tgcactcgcg atcgtggacc tgagcatgcc ggtgatggac ggttacgata ccatcgcctg
     1141 gctgcgcggc catgcgccca gggtgctgcc tctggccctc acattcgatg gcaacgacga
     1201 ggtgatgatg cgcgctgtgc atgcgggggc acgcggcttc atcctgaaaa gtgcgcgccc
     1261 cgcgatgctg aagaacgcac tggacagcct catcctatcc ggatggtata attcagagga
     1321 actcgtggat gttcgcctgc gcaactccga acggcgcagg cacgaggaac aggagcggca
     1381 agtggtcgtt gagcgcatca gcccgcgaga gatggaattc ctgcgattgg tgtgcgatga
     1441 agccgagttc acctatgaac agatcgcatc acgcatggga atacagcgtc gatcggtgga
     1501 caatttccgg atcgggctct tcgagaaatt caacatcaag agcaagaccg gcctggtgtt
     1561 gttcgccatg cgctggggac tgatcaacgg caaaagctag ttgaggccgc aaggccgatc
     1621 caacgggcct ggtcggggtc aatttctttg ccgggcacag ccgataaatg ggcgtaagcc
     1681 gtgtctcctt ggccatgatc cggcgaagaa caggcggaag agaggccgga ggacgcccga
     1741 aagctgatcc cgccccgtct ccaacggaac ggcaccacca acctgtactt ttcgccagaa
     1801 gtagcgacca tgttcacggt gatccacaac ttcgacgttc gtcccgggcg cgaacagg
//
LOCUS       DAOQOC010000662         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44225_length_1855_cov_3.641667, whole genome shotgun sequence.
ACCESSION   DAOQOC010000662 DAOQOC010000000
VERSION     DAOQOC010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44225_length_1855_cov_3.641667"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PL106_10470"
     CDS             <1..643
                     /locus_tag="PL106_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33533.1"
                     /translation="PGLFERFLGDSKLSSILQNLDKTVLISHFDQPLVAHKRIVCAVP
                     PLAEFEKGFELWTSKIVKLAQELTVPITVHCDQATGKAIEKVLARSRHKAPLAIMPFS
                     EWEDFLVLSRSVRESDLFILVAARKGAASYKSVLDDLPSKMEKYFATNNRIVIYPQQF
                     HDGRVPDEYADFTAEPLHIGIDAVQKLGKSIGSIFKKDDAAGAAREQDPDGPL"
     gene            772..1158
                     /locus_tag="PL106_10475"
     CDS             772..1158
                     /locus_tag="PL106_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNA33534.1"
                     /translation="MSYHFSKTISADFDAAIAKVTEALKQEGFGILTEIDMQAALKNK
                     LGVDFRKYKILGACNPPFAHQAVTAEPHIGTMLPCNVIVQEHAPGQVEVSAVDPVASM
                     MAVKNEGLGAIAGEVRDKLKRVVEGL"
     gene            complement(1299..>1855)
                     /locus_tag="PL106_10480"
     CDS             complement(1299..>1855)
                     /locus_tag="PL106_10480"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HNA33535.1"
                     /translation="LYAAFLAGRHELGRGEEEQLGFRLAKRFQLKTLHCVDEWGRFTP
                     NIDSLFNGTDSLGALRFEAYFAADPDSALRRRSTAQVDRTGILGALREANDPAHIERD
                     LGNYLIGLFKYEAVPGDFTGVDFESGRWFNRNLRIFRNIQRIPVTPEDRILVIYGAGH
                     LNVLNLLFRSSPEYRFVGAEELLR"
BASE COUNT          376 a          575 c          565 g          339 t
ORIGIN      
        1 cccgggcctg ttcgagcggt tcctgggcga cagcaaatta tcgagcatcc tacagaatct
       61 cgataagacg gtattgatca gccatttcga ccagccgctg gtggcgcaca aacggatcgt
      121 gtgcgcggtg ccaccgctgg ccgaattcga gaagggcttc gaactctgga cgagcaagat
      181 cgtcaagctg gcccaggaac ttaccgtgcc catcaccgtt cattgcgacc aggccacggg
      241 caaggccatt gagaaggtgc ttgcccggtc aaggcacaaa gccccgcttg cgatcatgcc
      301 tttctccgaa tgggaggact ttctcgtcct ctccaggagc gtgcgcgaat cggacctgtt
      361 catcctggtg gcagcccgca agggcgcggc ctcgtacaag agcgtgctgg acgacctgcc
      421 ctcgaagatg gagaagtact tcgccaccaa caaccggatc gtcatctatc cccagcagtt
      481 ccatgatggc cgcgttccgg acgagtatgc cgacttcacg gcggaaccgt tgcacatcgg
      541 catcgatgcg gtacagaagc tggggaagag catcggtagt attttcaaga aggatgatgc
      601 cgccggggcc gcccgtgagc aggacccgga tgggccgcta tagcgccaac ggggatccca
      661 cagtgcttcc ggcccgatgc tccgatgccg gatcccggtg acattcctta cccgggggaa
      721 gctctacagt gatgactttc gcacccggaa atcgaccgga ccacacctcc catgagctac
      781 catttctcca aaacgatcag cgccgacttc gatgcggcca ttgcaaaagt caccgaggca
      841 ctcaagcagg aaggcttcgg catcctcacc gagatcgaca tgcaggccgc gctgaagaac
      901 aagctgggcg tggatttccg caagtacaag atccttgggg cgtgcaaccc gccctttgcg
      961 caccaggccg taaccgccga gccccacatc ggtaccatgc tgccctgcaa cgtgatcgtt
     1021 caggagcatg cccccggcca agtggaagtc agtgccgtgg accccgtggc cagtatgatg
     1081 gcggtgaaga acgaagggct cggcgccatc gccggtgaag tgcgggacaa actgaagcgg
     1141 gtggtggagg ggctgtgaat tggtgtacgg tgcgccttca cgcttaccgg gaatggaggc
     1201 aactgctgct catgcgcctc agcttgggtt tccctggacc tgcgcgatga tgctggccac
     1261 aagaccggct gttcatggac caggttgtgg ccttccgttc accgcaacaa ttcctccgct
     1321 cccacgaacc ggtattccgg cgagctgcgg aagagcaggt tgagcacgtt caggtgcccc
     1381 gccccataga tcaccaggat cctgtcctcg ggtgtgacgg ggatgcgctg aatgttgcgg
     1441 aagatgcgca ggttgcggtt gaaccagcgg ccgctctcga aatccacacc ggtgaaatcg
     1501 cccggcaccg cctcgtactt gaagagaccg atgaggtagt tgccgaggtc gcgttcgatg
     1561 tgtgcggggt cgttggcctc tcgcaaagcg cccaggatgc ccgtgcgatc cacctgcgcg
     1621 gttgagcgcc tgcgcagcgc actgtccgga tcggcggcga agtaggcttc gaaccgcagc
     1681 gcacccaagc tgtccgtacc gttgaacagg gagtcgatat tcggcgtgaa tcgcccccat
     1741 tcatccacgc agtggagcgt cttcagttgg aagcgcttcg ccagccggaa gccgagctgc
     1801 tcctcctccc cacgccccag ctcatgccgt ccggccagga aggcggcata caacg
//
LOCUS       DAOQOC010000663         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44240_length_1855_cov_2.516667, whole genome shotgun sequence.
ACCESSION   DAOQOC010000663 DAOQOC010000000
VERSION     DAOQOC010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44240_length_1855_cov_2.516667"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PL106_10485"
     CDS             complement(<1..987)
                     /locus_tag="PL106_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA33536.1"
                     /translation="MTDRKLRPESLMMSYGYKPELSQGAVKCPIFQTSTFVFQKAEDG
                     KAFFEVAYGLREQGEKEELGLIYSRLNNPDLEILEDRLGLWENAGDCAVFESGMGAIT
                     TTLLEFLSPGDLLLFSNPLYGGTDHFIKQILTRFGVEVLGFYPWEQDRLEEIVRKSGK
                     ADRLKMVYVETPANPTNILIDIQACGRLAKRFSTADKPVLLAVDNTYMGPLWQHPMEQ
                     GADLVLYSATKYIGGHSDVIAGACLGSKELMARVKGMRSFLGCMAGPWTGWLLLRSLE
                     TLKPRMETQERGAAAVAAFLRDHPKVKQVHYLGFIDEPAQQAIYKNQCLSAGA"
     gene            complement(1254..1571)
                     /locus_tag="PL106_10490"
     CDS             complement(1254..1571)
                     /locus_tag="PL106_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNA33537.1"
                     /translation="MKGDVVVIPFPFSDLSGSKRRPALVVGDWGGTDVMLCQITSQAK
                     RDGLEVALNADDLLSGGLPIASHIRPNKSFTAERSLILYVAGKVNMDKYQQVVRGIVA
                     LIS"
     gene            complement(1568..1720)
                     /locus_tag="PL106_10495"
     CDS             complement(1568..1720)
                     /locus_tag="PL106_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33538.1"
                     /translation="MAWLQQCSMQEKKKILRELLDDTAAITIASEPSLAKDWDDKEED
                     EAWKDL"
BASE COUNT          323 a          597 c          540 g          395 t
ORIGIN      
        1 ggcgcccgca ctgaggcact ggttcttgta gatggcctgc tgcgcgggtt cgtcgatgaa
       61 gcccaggtag tggacctgct tcaccttggg atggtcccgc aggaacgcgg ccacagctgc
      121 ggcaccgcgc tcctgggtct ccatgcgcgg cttcagcgtt tccaggctgc gcagcaggag
      181 ccaaccggtc catgggccgg ccatgcagcc caggaagctg cgcatccctt tcacacgggc
      241 catcagctcc ttgctgccca ggcaggcccc ggcgatcaca tcgctatgac cgccgatgta
      301 cttggtggcc gagtacagta ccaggtcggc gccctgctcc atcgggtgct gccacagcgg
      361 acccatgtac gtgttgtcca cggccagcag caccggtttg tccgcggtgc tgaaccgctt
      421 ggccaggcgg ccgcatgcct ggatgtcgat caggatattg gtaggattcg cgggagtttc
      481 cacgtacacc attttcaagc ggtcggcctt gccgctcttg cgcacgatct cctccaagcg
      541 gtcctgctcc cacgggtaga agcccagcac ctccacgccg aagcgggtga ggatctgctt
      601 gatgaagtgg tccgtgccgc cgtacagcgg gttgctgaac agcagcaggt cgcccggcga
      661 gaggaactcc agcagggtgg tggtgatggc gcccatgccg ctctcgaaca cggcgcaatc
      721 ccccgcgttc tcccacaggc ccaaccggtc ctccaggatc tccaggtcgg ggttgttcag
      781 ccggctgtag atcaggccca gttcctcctt ctcgccctgt tcgcgcagcc cgtaggccac
      841 ctcgaagaag gccttgccat cctcggcctt ctggaaaacg aaggtgctgg tctggaagat
      901 gggacatttc acggcgccct ggctgagctc gggcttgtag ccgtagctca tcatcaggct
      961 ctcggggcgg agcttgcggt cggtcatgca acgaaggtac ccacgctcct atgggaggcg
     1021 ctgacattcg gcacctgcgg gtggttggtc ggctgatccc tcacaccgcc accttgccca
     1081 tgcccgccac agcctcaggc ggtggcgggc ttcttcgcct tcccgttccc attgctcttc
     1141 tccttctcat cgatcggttt gcgcaacacg atcttcccat cgaagatgtc gatcaccatg
     1201 ggggcgggct gtgcttgact tcaatgatcc agcggcttca gacccaaagc ggatcacgag
     1261 atcaatgcca caattccccg aaccacttgc tgatacttgt ccatattgac tttaccagcc
     1321 acataaagga tcaaggaccg ctctgcggtg aagctcttgt tgggccggat atggctcgcg
     1381 atgggaagcc cgccggacag gagatcatcc gcgttcaggg caacctccaa cccgtcccgt
     1441 ttggcttggc tggtgatctg gcacagcatg acatccgtgc caccccagtc accgacaacc
     1501 aatgccggtc tgcgcttgct tccgctcagg tccgagaagg ggaacgggat caccaccaca
     1561 tcgcccttca taggtccttc catgcttcat cctcttcctt gtcgtcccag tccttggcca
     1621 agctgggttc gctggcgatg gtgatcgccg ctgtatcgtc cagcaattcg cggaggatct
     1681 tcttcttttc ctgcatcgag cattgctgca gccacgccat cacttggttg atgttgaccg
     1741 gaaggttgtg ttcggaggcg tgcatggctg gtgtatggat acgaagatag tacagatgag
     1801 acctttgtca gtgatccctg gcgcccgaac aactggccac taacgaacct cggac
//
LOCUS       DAOQOC010000664         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44362_length_1852_cov_3.903728, whole genome shotgun sequence.
ACCESSION   DAOQOC010000664 DAOQOC010000000
VERSION     DAOQOC010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44362_length_1852_cov_3.903728"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PL106_10500"
     CDS             <1..509
                     /locus_tag="PL106_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33539.1"
                     /translation="PHNPCFLGDQLHVSWYEDGYWLWSVADPHNAQLLGWYDMHPEPY
                     SFSYKGAWGVYPYLPSGHVLISEMQHGLFVLDISQALSLGAGMVTGQERLRVRSGNSH
                     IIVDLPKGARITSIADALGRQVPVDIRGTTNGGTIVDTRNFAPGIFTITATDSDRRWS
                     TTFLNILE"
     gene            506..>1852
                     /gene="gatB"
                     /locus_tag="PL106_10505"
     CDS             506..>1852
                     /gene="gatB"
                     /locus_tag="PL106_10505"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNA33540.1"
                     /translation="MSTPWQEKYELVVGLEVHAQLLTESKAYSSEANAYGDRPNTLVG
                     PVTLGLPGTLPVPNTKVIDLAIRLGLACGCDIAPRMYYARKNYFYPDLPKGYQITQDK
                     TPICTGGKITIPDGAGEKDIRITRIHMEEDAGKSIHDVDPFNTLVDLNRAGVPLVEIV
                     SEPDIRSAQEAYDYLVEVRRLVRYLDVCDGNMEEGSLRCDANISIRPKGSPEFGTRTE
                     VKNLNSFRNVARAIEYEAQRQSEVIDGGGVIHMETRTFDAAKGITIGLRSKEMAHDYR
                     YFPEPDIQPLTVSEAKKEAIRRAMPPLPRELREKYVSTLGLSAYDAANLTDDRDTALY
                     YEETLKGTRNAKAAANQVMGPVRGWVNERGLTIREFPISPVRLASLVELIESGKVSHT
                     LAAQKLFPLMLVQDGTAEELATAHGLLTDTREDAIEAALRQAMKKYPDKVDAFRAGN"
BASE COUNT          394 a          589 c          577 g          292 t
ORIGIN      
        1 taccgcacaa cccctgtttc ctgggcgatc aactgcatgt gtcctggtac gaggacggtt
       61 actggctgtg gagcgtggcc gacccgcaca acgcgcaact gctgggctgg tacgatatgc
      121 accccgaacc gtacagtttc agctacaagg gggcatgggg cgtgtacccc tacctgccca
      181 gcggccatgt cctcatcagc gagatgcagc acggcctctt cgtgctcgat atcagccaag
      241 ccttgtcgct cggtgctggg atggtcaccg ggcaggaacg cctgcgtgtg cgctccggga
      301 acagccacat catcgttgac cttcccaagg gcgcacggat caccagcatc gccgatgccc
      361 ttggccgaca agttccggtg gacatccgtg gcaccaccaa cggcggaacc atcgtggata
      421 cgcgcaactt cgcccccggc atctttacca taacagccac cgacagcgac cgtcgctgga
      481 gcaccacttt cctgaacatc ttggaatgag cacgccctgg caggagaaat acgaattggt
      541 ggtgggcctg gaggtgcatg cgcagctgct caccgaaagc aaggcgtaca gcagcgaggc
      601 caacgcgtac ggtgaccgtc cgaacaccct ggtcgggccg gtaacattgg ggctgcccgg
      661 cacacttcca gtgcccaaca ccaaggtgat cgacctggcc atacggctgg gcctggcctg
      721 cgggtgcgac atcgcaccac gcatgtacta tgcccgcaag aactatttct acccggatct
      781 gcccaagggc taccagatca cgcaggacaa gacgcccatc tgcacgggtg ggaagatcac
      841 cataccggac ggtgcggggg agaaggacat ccgcatcacc cgcatccaca tggaagagga
      901 cgccggcaag agcatccacg atgtggaccc tttcaacacc ctggtggacc tgaaccgcgc
      961 cggggtgccc ttggtggaga tcgtgagcga acccgatatc cgctcggcgc aggaggccta
     1021 cgattacctc gtggaagtgc gccggttggt acgttacctc gatgtgtgcg acggcaacat
     1081 ggaagagggc agcctacggt gcgacgccaa tatcagcatc cgcccgaaag gttccccgga
     1141 attcggtacg cgcaccgagg tgaagaacct gaacagcttc cgcaacgtgg cgcgggccat
     1201 cgaatatgag gcgcagcggc agagcgaagt gatcgatggg ggcggcgtca tccacatgga
     1261 gacacgcacg ttcgacgccg ccaagggcat caccatcggg ttgcgcagca aggagatggc
     1321 gcacgactac cggtacttcc ccgagcccga catccagcca ctgacggtga gcgaggcgaa
     1381 gaaggaggcc atccgccggg ccatgccccc gctgccgcgc gagctgcgcg agaagtacgt
     1441 gtccacgctg ggcctcagcg cctacgacgc cgccaacctc acggacgatc gcgataccgc
     1501 gctctattac gaggagacct tgaaaggcac acgcaacgcc aaggccgcgg ccaaccaggt
     1561 gatgggtccg gtacgtggtt gggtgaacga gcgcggcctc accatccggg agttcccgat
     1621 cagtccggtg cgattggcct cgttggtgga actgatcgaa agcggcaagg tgagccacac
     1681 gctcgccgcc cagaagctct tcccgttgat gctggttcag gatggcacgg ccgaggaact
     1741 ggccaccgcc cacggcctgc tcaccgacac gcgggaggat gccatcgagg ccgcgctgcg
     1801 gcaggcgatg aagaaatatc ccgataaagt ggatgcgttc cgtgccggga ac
//
LOCUS       DAOQOC010000665         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44473_length_1850_cov_2.979387, whole genome shotgun sequence.
ACCESSION   DAOQOC010000665 DAOQOC010000000
VERSION     DAOQOC010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44473_length_1850_cov_2.979387"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PL106_10510"
     CDS             <1..252
                     /locus_tag="PL106_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33541.1"
                     /translation="GMTFDTKGNQSQAVDRILARVSELSAADRRAFMKAIRQQERKDD
                     IAWLHKVWGKVSLKDSEITRIVEEVRTEMHASRSPKARR"
     gene            275..646
                     /locus_tag="PL106_10515"
     CDS             275..646
                     /locus_tag="PL106_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNA33542.1"
                     /translation="MIGKRLRELDVLLMEELFQLCLSTRALAEIAEVTRRPKLRKYFT
                     QARAEESIERLGRVAKVLKREPKVIPICRDAKDDFLLALTKAAKADLLVTGDDDLLVL
                     KKYGRTRIVTPAEFLKDHVKA"
     gene            768..>1057
                     /locus_tag="PL106_10520"
     CDS             768..>1057
                     /locus_tag="PL106_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33543.1"
                     /translation="MADRFHRRSIRLKGYDYTQAGAYFVTACTHERAHLFGDVDNGMM
                     APNAMGMVAQRCWDAIPEHMPMVALDEFVVMPNHIHGIIVITDPASSVGNGRG"
     assembly_gap    1058..1067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1172..1375
                     /locus_tag="PL106_10525"
     CDS             1172..1375
                     /locus_tag="PL106_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33544.1"
                     /translation="MQTFKAAVSRQAVKDGLVARGTPIWQRGYIEHIIRNGASHDRIA
                     NYIRNNPANWNAGDFERGEAPAG"
     gene            complement(1451..>1850)
                     /locus_tag="PL106_10530"
     CDS             complement(1451..>1850)
                     /locus_tag="PL106_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNA33545.1"
                     /translation="VVPKFTVIVGNSYGAGNYAMCGKAYDPRLIVGWPSAQVAVMGGD
                     QAAKVLLQIEVAALKGRGEEITKEREEEILGKIRARYEEAMSPYYAAARLWLDAIIDP
                     LETRTWISMGIEAASQAPATREFNMGVLQT"
BASE COUNT          381 a          532 c          573 g          354 t
ORIGIN      
        1 gggatgacct tcgacaccaa aggaaaccag agccaggccg tggatcggat cttggcgcgc
       61 gtcagcgaac tatctgctgc tgataggcgt gccttcatga aggccattcg gcagcaggaa
      121 cgcaaggatg atatcgcgtg gctgcacaag gtctggggca aggtgtcgct gaaggacagc
      181 gagatcacgc gcatcgttga agaagtaagg acggagatgc atgcctcgcg ctcgcccaag
      241 gctcgtcgtt gataccaacg ttttgatcag ttggttgatc ggaaagcggc tgcgcgaact
      301 ggatgtcttg ctgatggagg aactctttca actatgcctc tcaacgcggg ccttggcaga
      361 gatcgccgaa gtgacgcgac ggccgaagtt gcgcaagtac ttcacgcagg ccagggcgga
      421 ggagagcatc gagcgcttgg gccgtgtggc gaaggtgctg aagcgcgaac cgaaggtgat
      481 ccccatctgc cgcgatgcga aggatgactt cctgctcgcg ttgaccaagg ccgcaaaggc
      541 ggatctgctg gtgaccggtg atgatgacct gctcgtgctg aagaagtacg gtcggacgag
      601 aatagtgacg cctgcggagt tcctgaagga tcatgtgaag gcgtagtgga ggttacgcgc
      661 atggtgtacc gcgacgggtt gttgccccac ggcacgccgc cctgagcttg tcgaagggtg
      721 gcaacggtat gggcgggaaa tttcccgccc ggtccgatat tcgtgatatg gccgaccgtt
      781 tccaccggcg atccatccgg ttgaaaggat acgattacac ccaagccggt gcgtatttcg
      841 tgacggcatg tacccacgaa cgtgcgcacc tgttcggtga cgtggacaac gggatgatgg
      901 cgccgaatgc aatgggcatg gtggcgcaac gatgttggga tgccattccg gaacacatgc
      961 ccatggtggc attggatgaa ttcgtcgtga tgccgaacca catccatggg atcatcgtga
     1021 tcacggatcc ggcgtcgtcg gtcgggaatg gcaggggnnn nnnnnnnccc ctgccattcc
     1081 cgggaaaccg acgccgccgg aaatgggcaa cgatcccccg caacgacgac aaccaattgt
     1141 caccatggtc ccaaattcat tgggccacat catgcaaacg ttcaaggccg cggtttcgcg
     1201 gcaggccgtg aaggatggtt tggtcgcgcg tggcacaccg atctggcaac gcgggtatat
     1261 tgaacacatc atccgcaatg gggcgtcgca tgacaggatc gccaattaca tccggaacaa
     1321 cccggcgaat tggaacgccg gggattttga aaggggcgaa gcgcccgccg ggtaacaggg
     1381 gcgagagaat tcccgcccca acgccgagaa ccccgccaat tgtgggaagg tccggttcaa
     1441 ttggtagggg tcacgtctgc aacaccccca tattgaactc tcttgtcgcc ggtgcctggc
     1501 tcgccgcctc gatccccatt gatatccacg tccttgtctc caacggatca atgatcgcat
     1561 ccaaccacaa cctggccgct gcgtaatacg ggctcatcgc ctcctcatag cgtgcccgga
     1621 tcttcccaag gatctcttcc tcccgctctt tggtgatctc ctcgccgcgg cctttcaacg
     1681 ccgccacttc gatctgcagc agcaccttgg cggcctggtc gccgcccatc acggccacct
     1741 gggcgctggg ccagcccacg atgaggcgcg ggtcgtaggc cttgccgcac atggcgtagt
     1801 tgccggcgcc gtagctgttg cccacgatca cggtgaactt gggcaccacg
//
LOCUS       DAOQOC010000666         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44518_length_1849_cov_3.480491, whole genome shotgun sequence.
ACCESSION   DAOQOC010000666 DAOQOC010000000
VERSION     DAOQOC010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44518_length_1849_cov_3.480491"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PL106_10535"
     CDS             complement(<1..960)
                     /locus_tag="PL106_10535"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNA33546.1"
                     /translation="MHWIGIDVSKAKLDVALLNDQGAVLDELEVANTDHGLSGLLERW
                     GEAHGVNKAGCLVCLEPTGHYSNQTLRTLVMLEYPTWLAHPRDIQKSIGLQRGKNDRV
                     DARRIADYARRFVDKAHLFTLKDLELEKVGHLLTRRRQLVKERKRYMVQLTDRNPCLG
                     ELRERFDQFDKQQIGALKDAIKEVEEAIGTELCKVQENARIYELIRSVPGVGLVLAAY
                     LMVKTAAFKRYPTPRALACQAGVVPYEHISGTSVRGRARISHNADKTMKSLLHMAAIG
                     TIRRSGDLQAYYLRKVAEGKPKMVVINALRNKLIHRIYAVVDRG"
     gene            complement(1289..1699)
                     /locus_tag="PL106_10540"
     CDS             complement(1289..1699)
                     /locus_tag="PL106_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNA33547.1"
                     /translation="MYPPELTAPMAAELVASGFEELKTPEQVDKALGQPGTVLCMVNS
                     VCGCAAGAARPGVRAALSGNKRPDKLVTVFAGVDTDAVIRARQHCLPYPPSSPSIALF
                     KDGKLVHFVERHHIEGRSAGMIAEHLNTVFDEFC"
BASE COUNT          350 a          573 c          529 g          397 t
ORIGIN      
        1 accgcggtca accaccgcat agatgcgatg gatgagcttg ttccgcagag cgttgatcac
       61 gaccatcttc ggtttccctt ccgcgacctt cctcagatag tacgcttgca gatcaccgga
      121 ccgtcgtatc gtgccgatag ccgccatgtg cagtaggctc ttcatggttt tgtccgcatt
      181 atgtgatatg cgggctcttc ctctgacgct ggtacctgag atgtgctcgt acggaacgac
      241 cccggcttgg caggccaggg cacggggcgt ggggtacctt ttgaaggcgg cggtcttcac
      301 catcaagtaa gcggccagta caaggccaac gccaggcacc gagcggatga gctcatagat
      361 gcgtgcgttc tcctggactt tgcatagttc ggtcccaata gcctcctcca cttccttgat
      421 cgcatccttc agtgcgccga tctgctgctt atcgaactgg tcgaaccgct cgcgcagctc
      481 gcctaagcac gggttgcggt ccgtcagttg aaccatgtac ctcttgcgct ctttcaccag
      541 ctgcctgcgc cgggtgagga gatgcccgac tttctccaac tccagatcct tcagcgtgaa
      601 caggtgcgct ttgtccacaa agcgccgcgc atagtcggct atccgccggg cgtccaccct
      661 gtcgttcttc ccgcgctgta ggccgatgct cttttggatg tccctgggat gggccagcca
      721 cgtggggtac tccagcatca ccaaggtgcg taaggtctgg ttggagtagt gcccggtcgg
      781 ctccaggcag accaggcagc cagccttgtt cacaccatgc gcctctcccc atcgctccaa
      841 caagcccgag agcccatgat cagtgttagc tacttcgagc tcatcgagca ccgcgccctg
      901 atcgttcagc aaggccacat ccagcttggc tttgctcaca tcaataccta tccaatgcat
      961 gtccaactta gttttgtggt gcaacctgtg gccactgaag ggactgagcg gcagaccttg
     1021 aactcgatga ctctaatggg ccttgatgcc cggtattcca tatgagtctc acggtctgaa
     1081 cagaccaagg ggtccactaa gacggatggg gcttgcaccc cgtcagtagt atcggttcac
     1141 cccaaggtcc agctcagttc tcttcggcgg tcctgtcaag cgccaagttc gttactcagc
     1201 acaggggcaa gtctagagtc agtattcacc cgaaaacagg aagcccggcc atggaccttc
     1261 cacagccggg cattccatgg aggtacgatc agcagaactc gtcgaacacc gtgttgaggt
     1321 gctcggcgat catgcctgca ctgcggccct cgatgtggtg gcgttcaacg aaatgcacca
     1381 gcttgccatc cttgaagagc gcgatgctgg ggctgctggg cgggtagggc aggcagtgct
     1441 ggcgcgcacg gatcaccgca tcggtgtcca ccccggcgaa cacggtcacc agtttgtccg
     1501 gacgcttgtt gccggagagc gcagcgcgca caccggggcg tgccgcaccg gcggcacagc
     1561 cgcaaacgct gttcaccatg cagagcacgg taccgggctg gccaagggcc ttgtccactt
     1621 gttcgggggt cttcagttct tcaaaaccgg aagccaccag ttcggcggcc atcggggcgg
     1681 tgagttcagg agggtacatg atcggttcgg atccacctgg tggtggaacg acaaaggtac
     1741 ggtgccgacg agcggaccac tcggccgaat gatgcatccg atcctcctcg accgcctcca
     1801 cgtctttttc cgtggagacc accacgttga attacgctgg cgcccgctt
//
LOCUS       DAOQOC010000667         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44558_length_1848_cov_3.441718, whole genome shotgun sequence.
ACCESSION   DAOQOC010000667 DAOQOC010000000
VERSION     DAOQOC010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44558_length_1848_cov_3.441718"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PL106_10545"
     CDS             <1..419
                     /locus_tag="PL106_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNA33548.1"
                     /translation="LLKWADRNSMRFSVEARMPFLDHRLVEQVIPMAERWFIHRGMTK
                     QLLREAMRGRLPEAIRTRADKMGFETPEADWFRSPGFRALIQDMLHSTRFAQRGIVDV
                     GKAQRMYADHLAGRSDRSRDIWKLIHLEKWFTHFID"
     gene            419..1333
                     /locus_tag="PL106_10550"
     CDS             419..1333
                     /locus_tag="PL106_10550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA33549.1"
                     /translation="MGTERIAYKLVRWCGLPWLFREVLFRRSVRILTFHDPAPDAFRR
                     VLGYLHRHYGIITLDDHLSGKPLPAKPVVVTLDDGHIGNHALIPVLREFAVRPVVFVC
                     AGLVGTNRRFWFKHAARSGSSEGLKRISDAERLRILAADGFTPLHEYDVPQALGLEQL
                     KELASVADIGAHTLFHPCLHQCTSTVADAEIVGSKHVLEELLGHPVDGFAFPNGDYSS
                     RDAQLVSRAGFRYACTSDHGFNRSGGTFLLKRISVDDSGDIDALSVKASGVWALLRAV
                     MGRQRLHGEQPVPVEHATGTHHESLTNT"
     gene            1330..>1848
                     /locus_tag="PL106_10555"
     CDS             1330..>1848
                     /locus_tag="PL106_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33550.1"
                     /translation="MTVERFSDPAVLRASASYGSFLHFLESREHAGFFQSPSFMEVVK
                     PVPGFAPVLFLAHDSSGQVTGSLLGAYQQEGNGVKGWLSRRMLVQGGPLGDPTAADAL
                     ISALLADAKGKAIFVEFRNSFDTTELRPVFERKGFRYTPHLNFLLPVGSENEALQRLS
                     TARRRGLKTTLGA"
BASE COUNT          327 a          580 c          565 g          376 t
ORIGIN      
        1 acctgctgaa gtgggcggac cgcaactcca tgcggttctc cgtggaagca cgcatgcctt
       61 ttctcgacca tcgcctggtg gagcaggtga tccccatggc ggagcgctgg ttcatccatc
      121 gtggcatgac caagcaacta ttgcgcgagg ccatgcgcgg tcggctcccc gaggccatcc
      181 ggacgagggc ggacaagatg ggcttcgaga cgcccgaggc cgactggttc cgatcacccg
      241 ggttccgcgc cctcatccag gatatgctcc actccacccg cttcgcgcag cggggcatcg
      301 tggacgtggg caaagcgcag cgcatgtacg ccgaccacct ggccggacgc tcggatcgtt
      361 cgcgcgacat ttggaaactg atccacctgg agaagtggtt cacccatttc atcgactgat
      421 gggaacggag aggatcgcct acaaactcgt gcgctggtgc ggtctcccat ggctcttccg
      481 cgaggtgctc tttcgccggt ccgtacgcat cctcaccttc cacgacccgg ccccggacgc
      541 tttccggcgt gttctgggat atctgcatcg ccactacggc atcatcacgc tcgatgacca
      601 tctttcgggt aagcccttgc ccgccaaacc agtggtcgtt acgctggacg acgggcatat
      661 cggtaatcat gcgctcattc ccgtgctgcg tgaattcgct gtacgtccgg tggtcttcgt
      721 atgtgccggc ctggtgggca ccaatcgccg gttctggttc aaacatgccg cacgttcggg
      781 atcatcagaa ggtttgaagc gcatttccga cgcggaacgg ctgcgtatcc tggccgcgga
      841 cggcttcact cccctgcacg agtacgatgt cccccaggcg ttgggtctcg agcaactgaa
      901 ggagctggcg tcggtggcgg acatcggcgc ccacacgctt ttccatccat gcctgcacca
      961 gtgtacctca acggtcgctg atgccgagat cgtcggaagc aaacatgtgc tcgaagagct
     1021 gctcgggcat cccgtggacg gatttgcttt cccgaacggg gactattcct cccgcgatgc
     1081 ccaactggtg tcccgtgccg gtttccgcta cgcatgcacg tcggatcacg gtttcaaccg
     1141 atcgggcggg acatttttgc tcaagcggat cagcgtggat gattcaggtg atatcgatgc
     1201 cttgagcgtg aaggccagcg gtgtgtgggc actgctccgc gccgtcatgg gccggcaacg
     1261 cctgcacggt gagcaaccgg tgcccgtgga acatgcgacg ggaacgcatc acgaaagttt
     1321 gacgaacaca tgacggtgga acgcttctcc gatcctgcgg tgctgcgcgc ctcagcgtcc
     1381 tacggatcgt tcctgcactt cttggaaagc agggaacatg ccggcttctt ccagtccccg
     1441 tcgttcatgg aggtcgtcaa gcccgtgccg ggtttcgcgc ctgtgctttt cctcgcacat
     1501 gacagttccg gacaagtcac cggttcactc ctgggagcct accaacaaga aggtaatggg
     1561 gtgaagggct ggctgtcgag acgcatgctg gtacagggcg gaccgttggg tgatcccacg
     1621 gcggctgatg cgctgatctc agccttgctc gccgatgcga agggcaaggc catcttcgtc
     1681 gagttccgga actcgttcga tacgaccgaa ctacggcccg tgttcgagcg caagggcttc
     1741 cggtacacgc cgcacctgaa cttcctgttg ccggtagggt cggagaacga ggccttgcag
     1801 cgcctcagca ccgcccggcg gcgcggactg aagaccacgc tcggcgca
//
LOCUS       DAOQOC010000668         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_44940_length_1840_cov_3.454342, whole genome shotgun sequence.
ACCESSION   DAOQOC010000668 DAOQOC010000000
VERSION     DAOQOC010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44940_length_1840_cov_3.454342"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..230)
                     /locus_tag="PL106_10560"
     CDS             complement(24..230)
                     /locus_tag="PL106_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33551.1"
                     /translation="MRRSLRIGGRMGLRYAPFCFTEHGVLMLSSVLNSEAAIAVNIQV
                     IRVFTRKRELLANHKDILLAPIWR"
     gene            complement(265..339)
                     /locus_tag="PL106_10565"
                     /pseudo
     CDS             complement(265..339)
                     /locus_tag="PL106_10565"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(449..>1840)
                     /locus_tag="PL106_10570"
     CDS             complement(449..>1840)
                     /locus_tag="PL106_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNA33552.1"
                     /translation="DIMTDDISKPLNESGGAVLEVNAAPGFRMHLDPTGGLGRNVAEP
                     VVDMLFPPGTPSRIPIIAVTGTNGKTTSTRLTAHLMRSVGYKVGMTCSDGVYIMNRLL
                     MTGDCTGPASAQFVLKEPVVDMAVLETARGGLVRSGLGFEHCDVAIVTNVAADHLGLK
                     DIHTLEELAHVKSTVPRSVRRDGYAILNADDELVMAMRKQCDCKIALFSLDENNRLIR
                     QHCKLGGLAAIYENGFVTISKGEWKLRIEKAVNIPITYGGKAVFNIQNVLPAVLAAYV
                     QGVKIEELREALLTFVPVAAQTPGRLNLFQFKNFQVVVDYAHNPHGFEALGKFLSKIP
                     DSPKVGVIAGVGDRRDEDTVNLGRLSAQMFDEIIIRQDRNLRGKTDDEIIALMVKGIK
                     EVDPDKPFIIIKKEEEAIRHAISTAKPGSFLTLCSDVVPDALALVLKLKEEDDKVSFS
                     KEDIPNRNPMLVG"
BASE COUNT          327 a          559 c          553 g          401 t
ORIGIN      
        1 ccgcgcgaga gatttgtgga agtctagcgc cagatggggg ccagcaggat gtccttgtgg
       61 ttggccaaga gttcgcgctt acgggtgaaa acacggatga cctggatgtt gacggcgatg
      121 gccgcctcac tgttcagaac gctggagagc atcagcacgc cgtgctccgt gaagcagaac
      181 ggtgcgtaac gcagacccat gcgacctcca attctcaagc tcctgcgcat tcatttcgaa
      241 catgaagtcc tcagggaagc gctctttatt acgcctgacc gcttccttca gccttttggt
      301 ctccactccg taaagttccg cctgatcacg gtccaacata accttatggc cgcgaacggt
      361 gcggatcctg ccgataacga cttcatcgct caccaccagt atgttcttct tgatcgcgct
      421 catccggggt ctaccgcaac actaatgatc accccaccaa catcgggttc ctgttcggga
      481 tatcctcctt gctgaaggac actttatcat cttcctcctt cagtttcagc acgagggcga
      541 gcgcatcggg caccacgtcg ctgcagagcg taaggaagct gccgggcttg gcggtggaga
      601 tggcgtgacg gatggcctct tcctccttct tgatgatgat gaagggtttg tcgggatcga
      661 cctccttgat gcccttcacc atgagggcga tgatctcgtc gtccgttttg ccgcgaaggt
      721 tgcggtcctg gcggatgatg atctcgtcga acatctgcgc gctgaggcgg ccgaggttca
      781 cggtgtcctc gtcgcggcgg tcgcccacgc cggcgatcac acccactttc ggactgtcgg
      841 ggatcttgct caggaacttc cccagcgcct cgaagccgtg cgggttgtgg gcgtagtcca
      901 ccactacctg gaagttcttg aactggaaca gattcaagcg gcccggtgtc tgtgcggcga
      961 ccggcacgaa ggtcaacaag gcctcgcgca gttcttcgat cttcacgcct tgcacgtacg
     1021 cggcgagcac ggcgggcagc acgttctgga tgttgaagac ggccttgcca ccataggtga
     1081 tggggatgtt caccgccttc tctatgcgca acttccattc gcccttgctg atggtgacga
     1141 agccgttctc gtaaatggca gcgagtccgc cgagcttgca atgctggcgg atcaagcggt
     1201 tgttctcgtc caggctgaag agcgcgatct tgcaatcgca ctgcttgcgc atggccatca
     1261 ccaattcatc atcggcattg aggatggcgt agccgtcgcg gcgtacactg cgtggcacgg
     1321 tgctcttcac atgggcgagt tcctccaacg tgtggatgtc cttcagcccc aggtgatcgg
     1381 cggccacgtt ggtgacaatg gccacatcgc agtgctcgaa gcccaggccg ctgcgcacca
     1441 ggccgccccg agcggtctcc agcacggcca tgtccaccac aggttccttc agcacgaatt
     1501 gcgcgctggc ggggccggtg caatcgccgg tcatcagcaa gcggttcata atgtacacgc
     1561 cgtcgctaca ggtcatgccc actttgtagc ccacgctgcg catgaggtgc gcggtgaggc
     1621 gcgtgctcgt ggtcttgccg ttggtaccgg taaccgcgat gatggggatg cgactgggcg
     1681 tgcccggtgg gaagagcatg tccaccacgg gttcggccac gttgcgaccc aggccgccgg
     1741 tggggtccag gtgcatgcgg aagcccggtg cagcgttcac ctccagcacc gcgccgccgc
     1801 tctcgttcag cggcttgctg atgtcgtcgg tcatgatatc
//
LOCUS       DAOQOC010000669         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45057_length_1838_cov_2.622546, whole genome shotgun sequence.
ACCESSION   DAOQOC010000669 DAOQOC010000000
VERSION     DAOQOC010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45057_length_1838_cov_2.622546"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PL106_10575"
     CDS             complement(<1..672)
                     /locus_tag="PL106_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA33553.1"
                     /translation="MNIRLFKPSVGEEELEMIRQAFDRSWIGLGPMVTRFEEEWAAYS
                     GARAAIGVNSATAALHLAIASFRFPEGSRVLLPALTFAATASAVLYNRLIPVFVDSDP
                     VTLGLDLDDLERKFTKDCVAVIPVHYAGHPVPMDRLMPWARAHGMKVIEDCAHTAGTR
                     YKGRTLGTWGDIGCYSFEEKKLMTTGDGGMMVTDDPDLFKEVKAMRWVGIDKDNWRTA
                     QRYTGT"
     gene            818..>1838
                     /locus_tag="PL106_10580"
     CDS             818..>1838
                     /locus_tag="PL106_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA33554.1"
                     /translation="MSTLQTAPPYVIDPEKERDEIIARYKGLLRSIRGNRTPEHTRMV
                     RKAFNIAVEAHKEQRRKTGEPYIYHPIEVARICAAEIGLGATSVTAALLHDTVEDTDI
                     TLEEVKEMFGQTVADIVDGLTKISGLQFQTDSIQAENFRKVLLTLSRDVRVILIKLAD
                     RLHNMRTLEGMARDKQLKIASETLFLYAPLAHRLGLYNIKSELEDLALKFKEPDVYHD
                     IATRLKKGEAVRKRFISRFTVPIRESLDKEGFTYEIKGRPKSIHSIYNKMLKKNVTFE
                     EVYDVFAIRIIIDTKPELEKADCWRVYSFVTDHYQPSPDRLRDWISSPKANGYESLHT
                     TVMSPG"
BASE COUNT          387 a          565 c          594 g          292 t
ORIGIN      
        1 ggtgccggtg tagcgttggg cggtgcgcca gttgtccttg tcgatgccca cccaacgcat
       61 ggccttcacc tccttgaaca ggtcgggatc gtcggtcacc atcatgccgc catccccggt
      121 ggtcatcaat ttcttctcct cgaagctgta gcagccgata tcgccccagg tgccgagcgt
      181 gcgccccttg taccgcgtac cggcggtgtg cgcgcaatcc tcgatcacct tcatgccgtg
      241 cgcgcgtgcc cagggcatca accgatccat gggcacgggg tgacctgcgt aatggacggg
      301 gatcacggcc acgcagtcct tggtgaattt ccgctccaga tcgtccaggt ccaagcccag
      361 cgtaaccgga tcgctgtcca cgaacacggg gatcaagcgg ttgtacagca cggcggaggc
      421 cgtggcggcg aaggtgagtg cgggcaacag cacccggctc ccctcgggga accggaacga
      481 agcgatcgcc agatgaagcg cggcggtggc ggaattcacg ccgatggccg cccgggcacc
      541 ggagtaagcg gcccattcct cctcgaaccg ggtgaccatg ggccccagtc cgatccacga
      601 tcggtcgaag gcctggcgga tcatctccag ttcctcctcc cccacactcg gcttgaaaag
      661 tcggatgttc atgcgtggat gtgcaggggc aaaagtgggg ggttggcggg aaacagtcgc
      721 gttacaagcc tgatgtaccg ggctggcggg tgtagttttg acgcggtccc ggccgccacg
      781 ggcggtagaa ggaccgccgc acccgaggac cggatccatg agcaccttac agaccgcccc
      841 gccgtatgtg atcgaccccg aaaaggagcg cgatgagatc atcgcccggt acaagggctt
      901 gctgcgcagc atccggggga atcggacacc ggaacacacg cgcatggtgc gcaaggcgtt
      961 caacatcgcg gtggaggcgc acaaggaaca gcggcgcaag acgggcgagc cgtacattta
     1021 tcacccgata gaggtagccc gtatctgcgc ggcggagatc ggtcttggag ccacgagcgt
     1081 gacggcggca ctgctgcacg acacggtgga ggacacggac atcacgttgg aggaggtgaa
     1141 ggagatgttc gggcagacgg tggccgacat cgtggacggc ctcaccaaga tcagcggcct
     1201 tcagttccag accgacagca tccaggcgga gaacttccgc aaggtgctgc tcaccctgag
     1261 ccgcgacgtg cgggtgatcc tgatcaagct ggccgaccgg ctgcacaaca tgcgcacgct
     1321 ggagggcatg gcgcgcgaca agcaattgaa gatcgccagc gaaacgctct tcctgtacgc
     1381 gccgctggca caccgcctgg gcctgtacaa catcaagagc gagctggagg acctggcgct
     1441 gaaattcaag gaaccggacg tctaccacga catcgccacg cggctgaaga agggggaggc
     1501 cgtgcgcaag cggttcatca gccgcttcac ggtgcccatc cgcgagagcc ttgacaagga
     1561 gggcttcaca tacgagatca aggggcggcc caagagcatc cacagcatct acaacaagat
     1621 gctgaagaag aacgtcacct tcgaagaggt ttacgatgtc ttcgccatcc ggatcatcat
     1681 cgatacgaag ccggagttgg agaaggccga ctgctggcgg gtgtactcct tcgtcaccga
     1741 ccactaccaa ccgagcccgg accggttgcg ggactggata agctcgccga aggccaacgg
     1801 atacgagagc ctgcacacca ccgtgatgag cccgggcg
//
LOCUS       DAOQOC010000670         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45062_length_1838_cov_2.403253, whole genome shotgun sequence.
ACCESSION   DAOQOC010000670 DAOQOC010000000
VERSION     DAOQOC010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45062_length_1838_cov_2.403253"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PL106_10585"
     CDS             complement(<1..282)
                     /locus_tag="PL106_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HNA33555.1"
                     /translation="MGQRKKLAADARKEARKTVALASLKDVPTSPRKMRQSADTIRGL
                     DVEKALGILQYSTRHASQPLFKLLRSAIANWEAKNEGQKAGDTKLVVKTI"
     gene            complement(285..554)
                     /gene="rpsS"
                     /locus_tag="PL106_10590"
     CDS             complement(285..554)
                     /gene="rpsS"
                     /locus_tag="PL106_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNA33556.1"
                     /translation="MSRSLKKPPFIHYKLAKRVEDMQKSGKKSVLKTWSRASTISPDF
                     VGLTFAVHNGNKFIPVYVTENMVGHKLGEFAPTRTFRQHSGNNKS"
     gene            complement(599..1435)
                     /gene="rplB"
                     /locus_tag="PL106_10595"
     CDS             complement(599..1435)
                     /gene="rplB"
                     /locus_tag="PL106_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNA33557.1"
                     /translation="MAVRKLNPVTPGTRHKVITDFEGITTNVPEPSLTQGRVKSGGRN
                     NQGKMTMRYIGGGHKQRYRSIDSKRDKHGINGTVKTIEYDPNRSARIALIQYPDGEKR
                     YILAPAGLKVGQTIQNGKGVPPEVGNALPLGEIPLGTLVHNVELQPGKGGSFARSAGT
                     FAQLSAREGRYVTLKMPSGELRLVLTACMATVGTVGNAEHMLQKSGKAGRSRWQGRRP
                     RTRAVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGKKTRHPKSRSNRFILERINAKK
                     GA"
     gene            complement(1452..1745)
                     /gene="rplW"
                     /locus_tag="PL106_10600"
     CDS             complement(1452..1745)
                     /gene="rplW"
                     /locus_tag="PL106_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNA33558.1"
                     /translation="MSRIIIRPLVTEKMTKQTEKEGRYGFVVDRKSNKVEIKNAVEKE
                     YGVTVTGVRTAIVRGKDKTRYTKRNILRGGTSTWKKAFVTLKEGETIDLYSAI"
BASE COUNT          254 a          550 c          589 g          445 t
ORIGIN      
        1 gatggtcttt accaccaatt tggtatcacc ggccttctgc ccttcgttct tggcctccca
       61 gttggcgatg gcgctgcgca ggagtttgaa caggggctgg ctggcgtggc gcgtgctgta
      121 ctggaggatg cccagggcct tctccacatc cagaccccgg atggtatccg cgctctggcg
      181 cattttgcgg gggctggtgg gcacgtcctt caggctggcc agagccacgg tcttccgggc
      241 ctccttgcgt gcgtcagcag cgagtttttt gcgctgtccc atcgtcagga tttgttgttg
      301 ccggagtgtt gacggaaggt gcgggtgggg gcgaattcgc ccagcttgtg gcccaccatg
      361 ttctccgtca cgtacaccgg gatgaacttg ttgccgttat gcaccgcaaa ggtgagtccc
      421 acgaaatcgg gggagatggt gctggcgcgc gaccaggtct tcagcacgga cttcttgccc
      481 gacttctgca tgtcctccac gcgcttggcc agcttgtagt ggatgaacgg tggtttcttg
      541 agtgaacggc tcattggctc tcagttgggg tcgatcacga tcggcccgta cattattctc
      601 aggctccctt cttggcgttg atgcgttcca ggatgaaacg gttgctgcgg ctcttcggat
      661 ggcgcgtctt cttgcctttg gccagcaagc ccttgcggct gcgcgggtgc ccgccggagg
      721 cgcgcccctc accaccgccc atggggtggt ccaccgggtt catcaccacg gcgcgtgtgc
      781 gcggacggcg gccctgccag cggctgcgac cggccttgcc gctcttctgc agcatgtgct
      841 cggcgttgcc cacggtaccc acggtggcca tgcaggcggt aagcaccagg cggagttcgc
      901 cgctgggcat cttgagcgtg acgtaacgac cctcgcgagc gctcagctgg gcgaaggtgc
      961 cggcgctgcg ggcgaagctg ccacccttgc cgggctggag ctccacgtta tggaccagcg
     1021 tgcccaaggg gatctcgccc agcggcaacg cattgcccac ctcgggcggt acgcccttgc
     1081 cgttctggat ggtctgtccc actttcaggc ctgcgggcgc caggatgtag cgcttctcac
     1141 cgtcgggata ctggatcaac gcgatgcggg cactgcggtt cggatcgtac tcgatggtct
     1201 tcaccgtgcc gttgataccg tgcttgtcgc gtttgctatc gatggagcgg tagcgttgtt
     1261 tgtgaccacc gccgatgtag cgcatggtca tttttccctg gttgttgcgc ccaccgctct
     1321 tcacgcggcc ctgggtcagg ctcggctcgg gcacgttggt cgtgatgccc tcgaaatcgg
     1381 tgatcacctt gtggcgggtg ccgggggtga cgggattcag tttgcggact gccatggttc
     1441 caagctcgtt atcagatggc gctgtacaga tcgatggtct ctccttcctt cagcgtaacg
     1501 aaggccttct tccaggtgct ggtgccaccg cgcaggatgt tgcgtttggt gtaacgcgtc
     1561 ttgtccttgc cacgcacgat cgccgtgcgc acgccggtga cggtgacgcc gtactccttt
     1621 tccacggcgt tcttgatctc caccttgttg ctcttgcggt ccaccacgaa gccgtagcga
     1681 ccttccttct cggtctgctt cgtcatcttc tcggtgacga ggggacggat gatgatcctg
     1741 ctcatggctt acttggtgag ggtggactcc agcggggcga tggcatcctt gaggatgatc
     1801 accttgctgg cgttcatgat gtcataggta ttgatatc
//
LOCUS       DAOQOC010000671         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45249_length_1834_cov_4.286678, whole genome shotgun sequence.
ACCESSION   DAOQOC010000671 DAOQOC010000000
VERSION     DAOQOC010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45249_length_1834_cov_4.286678"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PL106_10605"
     CDS             <1..1393
                     /locus_tag="PL106_10605"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA33559.1"
                     /translation="VGHWINLSHCWGPTNEPGLDENCDVDDNVSDTPNTEGWTSCNLN
                     GATCGSPKDNVENYMEYSYCSKMFTNGQKTRMLAALNSNTASRNNLWTANNLALTGTT
                     EDQPLCAAAFLVDQQVVCGGSPVQFTDQSYHNPTNWNWSFPGGSPATSTEENPVVTYD
                     APGSYTVELTVGNGNSSVSTTQQAAVVVLSEPGAPLPWSESFEGMGSTLDPSQWMVEN
                     PGNDQYTFGLSEAAGYNSSHSVRMRNHGSTGGATDELLGPTLDLSGQDAVTLTFRYAF
                     ARRNGDNDDRLRFYVSNNCGDSWQLRKQFYGNSNLATVPDQNGVFTPGNADQWQLGSI
                     TNILPNYLVNNFRFKFAFESDAGNDLWIDDINIMGPVGINEASTMGSGLTVVPNPASD
                     VAFVELDLPRATEARIDLLDMTGRTVSTVRSGAIGAGGHRIELPVRNLQVGCYFVRLQ
                     RDGRTEVARLIVR"
     gene            1530..>1834
                     /locus_tag="PL106_10610"
     CDS             1530..>1834
                     /locus_tag="PL106_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNA33560.1"
                     /translation="MERQVILSPVLGFGAKLKEVPVLFKLRLTSLVVVSAVLGYWMGV
                     PKGGFDLMNALLLTLAGLLVTGASNAFNQVLEVDADSQMKRTAARPLVRGTLSMTEAV
                     "
BASE COUNT          374 a          564 c          558 g          338 t
ORIGIN      
        1 ggtgggccac tggatcaacc tgtcccattg ttggggtccc accaacgaac ccggactcga
       61 tgagaattgc gacgtggacg ataatgtcag cgatacaccc aataccgagg gctggacgag
      121 ttgcaacctg aacggtgcca cgtgcggatc gccgaaggac aatgtggaga actacatgga
      181 gtattcctac tgctccaaga tgttcaccaa cggacaaaag acacgcatgc tggccgcgct
      241 caacagcaac acggcctccc gcaacaactt gtggacagcg aacaacctgg ccctgaccgg
      301 taccaccgag gatcagcccc tgtgcgcggc cgccttcctg gtggaccagc aggtggtgtg
      361 cggtggaagc ccggttcagt tcacggatca gagttaccat aaccccacca actggaattg
      421 gagctttccg ggtggttcgc ccgccacgag taccgaagag aaccccgtgg tcacctatga
      481 tgcgcccggc agctacacgg tggaactcac cgtgggcaac ggcaacagca gtgtgagcac
      541 cacgcaacag gcggccgtgg tggtgctgtc cgagcccggc gcacccttgc cctggtccga
      601 atcgttcgaa ggcatgggat ccaccttgga ccccagccag tggatggttg agaaccccgg
      661 caacgaccag tacaccttcg gattgagtga ggctgccggt tacaacagtt cgcacagcgt
      721 gcgcatgcgc aaccacggca gtaccggagg cgcaacggat gaactgctcg gacccacgct
      781 ggacctcagt ggccaagatg ccgtcacgct caccttccgc tatgccttcg cgcgacgcaa
      841 tggggacaac gacgaccggt tgcggttcta cgtgagcaac aattgcggcg atagctggca
      901 gttgcgcaag cagttctacg gcaacagcaa cctggccacc gtgcccgatc agaacggggt
      961 gttcaccccc ggcaacgcgg atcaatggca gctcggctcc atcacgaaca tcctgcccaa
     1021 ttacctcgtg aacaacttcc ggttcaagtt cgccttcgag agcgatgccg gcaacgatct
     1081 ctggatcgat gacatcaaca tcatgggccc ggtggggatc aatgaggcga gcaccatggg
     1141 ctccggcctc accgtggtcc cgaatcccgc gagcgatgtg gcattcgttg agttggacct
     1201 gccacgggcc acggaggcgc gtatcgacct gcttgacatg accgggcgca cagtgagcac
     1261 cgttcgcagt ggtgcgatcg gtgccggggg ccatcgcatc gaattgcccg tgcggaacct
     1321 gcaggtcgga tgctatttcg tgcgcctcca gcgcgacggt cgcaccgaag tggcccgact
     1381 tatcgtgcgc tgaccgtaca ttgatcgtgt tcggagcccc gccctgcctg ccaggatggg
     1441 gcttcggaat tcaaggaaca tccctgcgcg gttcacttgg accattggat gcagcagcat
     1501 gctgacatcc gtctattttc gcccccgcat tggaacggca ggtcatcctt tctcccgtac
     1561 tgggcttcgg cgccaagctg aaggaagttc cggtgctttt caagctgcga cttacgtcct
     1621 tggtagtggt cagtgccgtg ctgggctact ggatgggagt gcccaagggg ggcttcgatc
     1681 tgatgaacgc gctactgctg accttggccg ggttgctggt gacgggtgcc agcaatgcct
     1741 tcaaccaggt actggaggtg gacgccgaca gccagatgaa gcgcaccgcc gcccgcccct
     1801 tggtgcgtgg cacgctgagc atgaccgaag cggt
//
LOCUS       DAOQOC010000672         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45258_length_1834_cov_3.510961, whole genome shotgun sequence.
ACCESSION   DAOQOC010000672 DAOQOC010000000
VERSION     DAOQOC010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45258_length_1834_cov_3.510961"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>1834
                     /locus_tag="PL106_10615"
     CDS             151..>1834
                     /locus_tag="PL106_10615"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNA33561.1"
                     /translation="MTPQQLISDVFLGECLSASGITYTGATGAIGTFSNGWGIGIPSG
                     IIMTTGSAPMAVGPNAIGSSGTNNSAGGNALLDGLVPGYTTQDASVFTFSFTPETDQV
                     TFSYVFASEEYPEFVCLGFNDVFGFFVSGPGYAPNTNIALVPGVFYPVTIDHVNASGG
                     CGPFFPAFYTGGNTSNTQYDGFTVPLTACINTVPCETYSITLAIADVGDGNYDSAVFL
                     AAQSFSAGVDLQIGAANGGSASSEAECMDQGTFVFTMDQPLDGATTLVYTITQAGSAV
                     YVPPIPITVTIPAGQTSITLPVSALPASLTADASTVTLVLNTADNPGVGCSCTSDTIT
                     ATLYLCDPVQLPVQWLGFDATLINGEQEVLCRWETATEQNNDHFTVERSRDGYGWQDI
                     GTVQAGDPITSPQYYELVDHGPLGGTGWYRVRQTDINGAFGHSEVRVVHRDPRFNLYP
                     NPGNGNFRIIGHEEGTLSIYDAMGRRVSFTLGPDGELPLPGAAVGTASPRVPSTPSTS
                     RRRSTARSIESSGPRSTNATRPADPDAAASPPRTTNAPRCGVVPVTARGRSLA"
BASE COUNT          328 a          580 c          581 g          345 t
ORIGIN      
        1 ggtgcccggt cgtacaacgg gacccccatc cttttcgact gcttcgcgct caccagcgat
       61 ggtggggact gtggaaccgc gaccggctgt gcaccgggcg tggctggcgg tgcgttgccg
      121 gggtcgggat ccgttcagat ccagcaaaca atgacccccc agcaattgat ctccgacgtg
      181 ttcctgggcg aatgcctatc ggcttcgggc atcacgtaca ccggggccac cggagccatc
      241 ggtacatttt ccaacggatg gggcatcggc atcccgtcgg gtatcatcat gaccaccggg
      301 tcggcgccca tggctgttgg gcccaatgcg atcggttcct ccggaacgaa caattccgct
      361 ggcggcaatg cgctgctgga tggcctggtc ccggggtaca ccacgcagga tgcgtcggtc
      421 ttcaccttct cgttcacgcc ggaaacggac caggtgacct tctcgtacgt gttcgcatca
      481 gaggagtatc ccgagttcgt gtgtctgggt ttcaacgacg tcttcggttt tttcgtgagc
      541 gggccgggct atgcgcccaa cacgaacatc gccctggtgc ccggtgtgtt ctatcccgtg
      601 acgatcgacc acgtgaatgc cagcggtgga tgcggcccgt tcttcccggc gttctatacg
      661 ggtgggaaca cgtcaaatac ccaatacgat ggattcaccg tgccgctcac ggcctgcatc
      721 aataccgtac cctgtgagac gtacagcatc accctcgcca tcgcggacgt gggcgacggg
      781 aactacgatt cggcggtgtt cctggcggcc cagagcttct cggcgggcgt tgatctgcag
      841 atcggtgctg ccaacggtgg tagtgcgagc agcgaagcgg aatgcatgga ccagggcacc
      901 ttcgtgttca ccatggacca gccactggac ggagccacca cgttggtgta caccatcacc
      961 caggcgggta gcgcggtcta tgtaccgccg atccccatca ccgtgaccat tcccgcaggg
     1021 cagaccagca tcaccctgcc ggtgagcgca ctgcccgcca gcctgacggc ggatgcgagc
     1081 acggtgacct tggtactgaa caccgcggac aaccccggag ttggctgcag ttgcacgagc
     1141 gacaccatca ccgccaccct ctatctctgc gatccggtcc agcttcccgt gcaatggctc
     1201 ggttttgatg cgacgctgat caatggggaa caggaggtcc tctgtcgttg ggagaccgcg
     1261 acggagcaga ataatgacca tttcacggtg gagcgctccc gcgacgggta tggatggcag
     1321 gatataggta cggtccaggc cggcgacccg atcacctcac cgcagtatta cgaactcgtg
     1381 gatcatggac ccttgggcgg taccggctgg tatcgtgtgc ggcaaacgga catcaacggg
     1441 gcattcgggc actcggaggt gcgcgtcgtt catcgcgatc cgcggttcaa tttgtatccg
     1501 aacccgggga acggcaattt ccggatcatc gggcatgagg agggaacact cagcatatat
     1561 gatgcgatgg gccgacgggt atcttttaca ctgggccctg atggggagct ccccctgccg
     1621 ggtgcggcgg tcggcaccgc atcgccgcgc gttccgtcga cgccgagcac atcgcgacgc
     1681 cgatcgaccg cccgctcgat cgaatcgagc ggcccgagat cgacgaacgc aactcgcccg
     1741 gcggatccgg acgccgccgc gtcgccgccg cgcacgacga acgcgccgag gtgtggagtc
     1801 gttccggtga ctgcgcgcgg ccggtcgctc gcgg
//
LOCUS       DAOQOC010000673         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45282_length_1834_cov_2.356380, whole genome shotgun sequence.
ACCESSION   DAOQOC010000673 DAOQOC010000000
VERSION     DAOQOC010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45282_length_1834_cov_2.356380"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1834
                     /locus_tag="PL106_10620"
     CDS             <1..>1834
                     /locus_tag="PL106_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNA33562.1"
                     /translation="TLLCTLHAQQWNWAADAGGGGNTDFCYGIGKDSQGSAYWVGTVS
                     GSADFGCGTLAPGSTIAGFVAKADANGVCQWVRGITVGFNEAWAYGIAIDAQDRIYVT
                     GSYDGNADFGNGITLNSMGSDDIFLARYDVDGNCIWSRRAGSSGSSDEARGVAVSEDG
                     GVFITGFSGGSTITFDNISIPNAGNARQIMVAHYDSLGTVQWAKASTGTAQGKTARAI
                     TVANDRLFITGQMGYNNASFDGIPLLPAPNTVGMYTLACDLDGNGLWARSYIGGDNEG
                     MGISADTLGNVFVAGRMWGNLYLPDDTLASVSGNDDIMLVALDRDGEHRWAQTTGSAQ
                     RDLAWGVATDGLGNAYVAAQFNSTIDFFGTPLSSLGSEDILISKFDGNGNVVWATRPS
                     GFQRDIPLTIHRQAAAPHELYFGGYFWGAITYGNTTIDDVQNGDAMIVAGIDTTFDVS
                     AIATNVCPGACDGSTEAFVNGSGPFSFQWNIGPATPELDGLCPGMYIVEVTDANGQTI
                     IDTVYVEEHADPGYTVHVANDSLWTTGGIGWAWYVDGNILVSDSSSAIAPQTGNYHAV
                     VTDAFGCTWSTDTVTIVLNVGIDEHALDLLTVRPNPAADRTTILW"
BASE COUNT          371 a          537 c          583 g          343 t
ORIGIN      
        1 accttgctat gtacccttca cgcccaacaa tggaactggg ccgccgatgc gggtggtggc
       61 gggaataccg acttctgtta cggtattggc aaggacagcc agggcagcgc ctattgggtg
      121 ggcacggtga gcggctccgc cgacttcggt tgcggcaccc ttgcaccggg gagcacgatc
      181 gctggtttcg tggcgaaggc ggatgccaat ggcgtgtgcc aatgggtgcg cggcatcacg
      241 gtgggcttca atgaagcatg ggcctacggg atcgccatcg acgcgcagga ccgcatctat
      301 gtcaccggca gctacgatgg caacgccgat ttcggaaatg gcatcactct gaattccatg
      361 ggttcggatg acatcttcct ggcccgctat gatgtggatg gcaactgcat ctggtcacgg
      421 cgggccggga gcagtggctc cagcgatgaa gcacgtggcg tggcggtctc cgaagatggt
      481 ggtgtgttca tcaccggctt cagcggggga agcaccatca ccttcgacaa catcagtatc
      541 ccgaatgctg gcaacgcacg gcagatcatg gtggcacact acgacagcct gggcaccgtg
      601 cagtgggcca aggcgagcac cggaacggcc cagggcaaga ccgcccgcgc gatcaccgtg
      661 gcgaacgatc ggcttttcat cacgggccag atgggataca acaacgcgag tttcgatggg
      721 atcccgctgc ttcccgcacc caacacggtg ggcatgtaca ccttggcctg cgacctcgat
      781 ggcaatggcc tgtgggcgcg cagttacatc ggtggcgaca acgagggcat gggcatcagt
      841 gccgatacgc tcgggaacgt tttcgtggcc ggaaggatgt gggggaacct ctatctgccg
      901 gacgataccc ttgcatcggt aagcggcaat gacgacatca tgctcgtggc cctggaccgc
      961 gatggtgagc accgctgggc gcagaccacc ggttccgcac agcgcgacct ggcatggggg
     1021 gtggcgacgg acggcttggg caacgcgtat gtggccgcac agttcaacag caccatcgac
     1081 tttttcggta caccgctctc atcgctcggc agcgaggaca tcctcatcag caagttcgat
     1141 ggcaatggca acgtggtgtg ggccacgcgc cccagcggct tccagcgcga catccccttg
     1201 accatccatc gccaggcagc agcaccgcat gaactgtatt tcggtggcta tttctggggc
     1261 gccatcacct acggcaacac caccattgat gatgtgcaga acggcgatgc catgatcgtg
     1321 gccggcatcg ataccacctt cgatgtgagt gccatcgcca cgaacgtgtg tccgggagct
     1381 tgcgatggta gcaccgaagc cttcgtgaat ggaagcgggc cgttctcctt ccagtggaac
     1441 atcggaccag caacgcccga gcttgacggc ctctgtccgg gtatgtacat cgttgaagtc
     1501 accgatgcga atggccagac catcatcgac acggtatacg tggaagaaca tgcggatccc
     1561 ggatacacgg tacacgtggc gaacgattcg ctctggacca ccggcggtat agggtgggct
     1621 tggtacgtgg atggcaacat cctggtgagc gacagttctt ctgcgatcgc cccgcagacc
     1681 ggcaactacc acgcggtggt gacggatgcc ttcggatgca cgtggagcac cgatacggtc
     1741 accattgtcc tgaatgtggg cattgatgag cacgcactgg acctcttgac cgtgcgtccg
     1801 aacccggcag cggatcgaac gacgatccta tgga
//
LOCUS       DAOQOC010000674         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45295_length_1833_cov_4.004499, whole genome shotgun sequence.
ACCESSION   DAOQOC010000674 DAOQOC010000000
VERSION     DAOQOC010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45295_length_1833_cov_4.004499"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PL106_10625"
     CDS             <1..646
                     /locus_tag="PL106_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNA33563.1"
                     /translation="PPQDHATVLELMNNLEKFLNDDALMTADPLVKMAIAHHQFESIH
                     PFYDGNGRTGRILNILYLVRHDLLELPILYMSRHIIQNKADYYRLLQQVRVDGNWEPW
                     ILYMLEAVARTSQQTIALIKGIKTLMQAFKQRLRNEQPRLYSQDLLNNLFRHPYTRID
                     FMMRDMDVTRITATRYLNVLVGMGLLQFVKVGRTNFYLNGRLFDLLRGGSISVS"
     gene            766..1038
                     /locus_tag="PL106_10630"
     CDS             766..1038
                     /locus_tag="PL106_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33564.1"
                     /translation="MKRILVSIILSPEGMATIRADQGLQRAEMAFIEKWKAEGILENF
                     YITTDRTGAFLIFNGVDGDRTKELIGGLPYFPYMSRVDYVELDKQF"
     gene            1326..>1833
                     /locus_tag="PL106_10635"
     CDS             1326..>1833
                     /locus_tag="PL106_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNA33565.1"
                     /translation="MAKIDSSDTRIAIFQRKEVRRTLHNNEWWFVIADVVAALTDSAD
                     VTQYVHKLRQRDPELAKGWVQIVHPLIVPTSGGAQKLNCANTEGLFRIIQSIPSPKAE
                     PFKCWLAKVGYERVQEIEDPELASKRTRAIYRAKGYSDDWIEKRMRSIAIRDELTDEW
                     KKRGVREQR"
BASE COUNT          428 a          546 c          497 g          362 t
ORIGIN      
        1 gcctccgcag gaccatgcga ccgtcctgga actgatgaac aacctggaga agttcctgaa
       61 cgacgatgca ctgatgaccg cagacccttt ggtgaagatg gccatcgcgc accaccagtt
      121 cgagagcatc cacccgttct atgacggcaa cgggcgcacg ggccgcatcc tcaacatcct
      181 ttacctggtt cggcacgatc tgctcgaact gccgatcctg tacatgagcc gccatatcat
      241 ccagaacaaa gcggactact atcgcttgtt gcagcaagta cgcgtggatg gcaactggga
      301 gccgtggatc ctttatatgc tggaagccgt cgcccgcact tcacaacaga ccatcgcatt
      361 gatcaagggg atcaagacgt tgatgcaggc gttcaaacag cgtctgcgca acgagcaacc
      421 gagactatac agccaggatc ttttgaacaa cctgttccgc catccttaca ccaggatcga
      481 cttcatgatg cgggatatgg atgtgacgcg gatcacggcg acgcgctact tgaacgtgct
      541 ggtgggcatg ggcctcttgc aattcgtgaa agtgggtcgc accaatttct atttgaacgg
      601 caggctgttc gacttgctcc gtggtggttc gatcagcgtg tcgtaatgaa cttcgacccg
      661 tgtccaccaa actcaacaag acccgagcct cagccgccag catggagggc ctcattccgg
      721 ctgacctccc acgtgcttgc taccattgca tcacattcca tacccatgaa acgcatcctc
      781 gtatccatca tcctttcgcc cgaaggcatg gccaccatcc gtgccgatca aggacttcaa
      841 agagccgaga tggccttcat cgagaagtgg aaggccgagg ggatcctcga gaacttctac
      901 ataaccacgg accgcaccgg ggctttcctc atcttcaacg gcgtggatgg ggatcgtacc
      961 aaggagctga tcgggggcct gccctatttc ccctacatgt ctagggtgga ttacgtggag
     1021 ttggacaagc agttctgatg aatgcccgcg ttttttcacc acagaggcac agaggacacg
     1081 gagaattcag atcacaatac cggaatgccg ggcgaacgaa gtaacagcgt acgctgtgcc
     1141 tctatggtga gttgaggtcg agaatacatt ccgaaagcgg tcgtacgatc caaccctgaa
     1201 ggccggtgcc caaacccaac aagacccgcg ccatcaccgc cagcgtcgat gccttcgttc
     1261 ggtaaccgca tttatcttgt tcaccccgcc tttgacgtat ttgccgtcaa cgccggagca
     1321 cgctcatggc caagatcgat tcttccgata cgcgcattgc catcttccaa cgcaaagagg
     1381 tacggcgcac cttgcacaac aacgagtggt ggttcgttat cgccgatgtg gtagcggccc
     1441 tcacggattc agccgatgtc acccagtacg tccacaagct gcggcagcgt gatccggaac
     1501 tcgccaaagg gtgggtacaa attgtacacc cccttatcgt tcccaccagt ggtggcgcgc
     1561 aaaagctcaa ctgcgcgaac accgaagggc tcttccgcat catccagtcc atcccgagcc
     1621 ccaaggccga gcccttcaag tgctggctgg cgaaagtcgg gtatgaacgg gtgcaggaga
     1681 tcgaggatcc cgagctggcc agcaagcgca cgcgggccat ctacagggcc aagggctata
     1741 gcgacgattg gatcgagaag cgcatgcgct ccatcgccat ccgcgacgag ctgaccgatg
     1801 agtggaagaa gcgcggtgtg agggagcaac gcg
//
LOCUS       DAOQOC010000675         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45374_length_1832_cov_2.930219, whole genome shotgun sequence.
ACCESSION   DAOQOC010000675 DAOQOC010000000
VERSION     DAOQOC010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45374_length_1832_cov_2.930219"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PL106_10640"
     CDS             <1..490
                     /locus_tag="PL106_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33566.1"
                     /translation="RGEAYEAWKKTPQFNLERSLWYRRALPAKYQGGNHVYGIDMKNN
                     SSNSRKSHLFLDIIRFFNEHGKNVWFYEYFTQLHNIEVNENPKSGAMEFGTMNKNQYN
                     DDLVFAIQYAFMSNECEMSQAMEIGTDVEEYRVVTKMFRNPVTLELEERQFKEKVMHV
                     SL"
     gene            487..1086
                     /locus_tag="PL106_10645"
     CDS             487..1086
                     /locus_tag="PL106_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33567.1"
                     /translation="MITDNSEQILMDLVSKNYVVYAPKSIKPIKRDYKELDDYEEFKA
                     LTSPELVFVWWFACQVSPIIDLPEEKRVMVAVDKAFKIKSQAEARKLEYKNMDFPGHI
                     KAAIKRMDRFDPGGRIRALHDDLHLLKQCESVIRKDITKAEMEEAAEWFKAVKAAREM
                     RDSILRKIERGGDGVDEKDNVLSSALEGVSSDFMKSQLA"
     gene            1083..>1832
                     /locus_tag="PL106_10650"
     CDS             1083..>1832
                     /locus_tag="PL106_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33568.1"
                     /translation="MNTAYTAGDTIFWPWLTIGAVTRPLNTTPKKYKDEHYDDRCGRY
                     YLGNILQQYRDYYIREYAANRQLVLNGPSSWGSSEAIRELLGSADNPTGRLALKYAIM
                     QPMHTRLVGVGSGISAAAKAQATTSNVYVRKESAMYQTLLRTIAAQEGPELAGAMAPM
                     GISPDEEKNLSMHENLWTDPYAKTMTDLVTAMGKRQKLEEKLAKIASNVALSGVAAVH
                     VFPNGNYMEAEVCDPGEVGWDTSAIKQDFSDG"
BASE COUNT          434 a          538 c          535 g          325 t
ORIGIN      
        1 caggggcgag gcatacgagg cgtggaagaa gaccccccag ttcaatctgg agcgatcgct
       61 gtggtatcgc cgggcactgc cggccaagta ccaaggaggc aatcatgtct acggtatcga
      121 catgaagaac aactcctcca actcccgcaa gagccacctg ttcttggaca tcatccgctt
      181 cttcaacgag cacggcaaga acgtctggtt ctacgagtat ttcacccagc tccacaacat
      241 cgaggtcaac gagaacccca agagcggggc catggagttc ggcacgatga acaagaacca
      301 gtacaacgac gacttggttt ttgccatcca gtacgccttc atgtccaacg agtgcgagat
      361 gtcgcaggcc atggagatcg gtaccgacgt ggaggagtac cgcgtggtga ccaagatgtt
      421 ccgcaacccc gtgacactgg agcttgagga gcgtcagttc aaggagaaag taatgcacgt
      481 ttccctatga tcaccgacaa cagcgagcag atcctcatgg accttgtatc caagaactat
      541 gtggtctatg ctcccaagag catcaagccc atcaagcgcg actacaagga gcttgatgac
      601 tacgaggagt tcaaggccct cacctctccg gagctggtgt tcgtatggtg gttcgcctgt
      661 caggtgtccc ccatcatcga ccttcctgaa gagaagcgcg tgatggtggc cgtggacaaa
      721 gccttcaaga tcaagagcca agccgaggcc cgcaagctgg agtacaagaa catggacttt
      781 cccggtcaca tcaaggccgc catcaagcgc atggatcgct tcgatccggg tggacgtatc
      841 cgcgcccttc acgacgacct gcatctgctc aagcagtgcg agagcgtgat ccgcaaggac
      901 atcaccaagg ccgagatgga ggaagcggcc gagtggttca aggccgtgaa ggcagcccgc
      961 gagatgcgcg acagcatcct gcggaagatc gagcgtgggg gtgacggtgt ggatgagaag
     1021 gacaacgtgc tatccagcgc cttggagggg gtttcctccg acttcatgaa gtcacaactc
     1081 gcatgaacac cgcatacacc gcaggtgaca ccatcttctg gccgtggctg accatcgggg
     1141 ccgtaacacg cccgttgaac accacgccca agaagtacaa ggacgagcac tacgacgacc
     1201 gctgtggtcg ctactacttg gggaatatcc tccagcagta ccgcgactac tacatccggg
     1261 agtacgccgc taaccgccaa ctggtgctca atggtccttc ctcatgggga tcctccgagg
     1321 ccatccggga actgttggga tcggccgaca accccacggg acgactggcc ctgaagtacg
     1381 ccatcatgca gcccatgcac acccgtttgg tgggtgtggg gtcgggcatc tcggcggcgg
     1441 ccaaggctca agccacgacg tccaacgtgt acgtgcgcaa ggagagcgcc atgtaccaga
     1501 ccctgctcag gaccatcgca gcacaggagg gtccggagtt ggctggggct atggcgccca
     1561 tgggtatctc tcccgacgag gagaagaacc tctccatgca cgagaacctt tggacggacc
     1621 cttacgccaa gacgatgacc gatctggtca ccgccatggg caaacgccag aagctcgaag
     1681 agaagctcgc caagatcgcc tccaatgtgg cgctctccgg agtggccgct gtccatgtgt
     1741 tccccaacgg taactacatg gaggccgagg tgtgcgaccc cggtgaagtg gggtgggata
     1801 cttcagccat caagcaggac ttctccgatg gg
//
LOCUS       DAOQOC010000676         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45469_length_1830_cov_3.090704, whole genome shotgun sequence.
ACCESSION   DAOQOC010000676 DAOQOC010000000
VERSION     DAOQOC010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45469_length_1830_cov_3.090704"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PL106_10655"
     CDS             complement(<1..417)
                     /locus_tag="PL106_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNA33569.1"
                     /translation="MSEKILKALLQLFAIIAKAEGGGISSRRGERTVSRFLLQQFTPE
                     QAAMHIATFDAFVEAFHTSGGTSDKGRKRTSLNSVKVLRICAQVNEELTQRQKVVVLF
                     HLLELIHANGKVEEQEREFVSTVAESFNIARQEFERC"
     gene            complement(578..997)
                     /locus_tag="PL106_10660"
     CDS             complement(578..997)
                     /locus_tag="PL106_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33570.1"
                     /translation="MRKTTRFFLLASGILLGSAASAQCDTIASICERHITAAYIPDGQ
                     FYRALLREDEMAEFDLTLYGGTTYRVAACSGLSDGNLIFSVLDRDRNVLYTNRDQNND
                     PYWDLVVSNTIDVTIEAQLDRSKAASGCAVMLIGFKK"
     gene            complement(1001..>1830)
                     /locus_tag="PL106_10665"
     CDS             complement(1001..>1830)
                     /locus_tag="PL106_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33571.1"
                     /translation="AKDTPAKADTLDTTAQGSGGGLMKVGGELFSIPSPVQTALLIRK
                     VGLPYDKALPMPVTTAANLAGNVQKALALGAYGADLAYLTVHKDGQRALTTLQTVQKL
                     SSDLNLSNAFDQELLDGFKKGLNNEDSLLRFTGKAFRSADQYLKNNKRDDVSALVLAG
                     GWIEGMFLTVSGTTEKTDATLNTRLGEQRETIENLISLLEAGGGTEQPVVSLITGLKD
                     VLAAYQGVTSTYEFRPATTDVAKKTTWINSVSSVTITAEQFKAIAEKVKALRATLTA"
BASE COUNT          324 a          552 c          554 g          400 t
ORIGIN      
        1 gcaacgttcg aattcctggc gtgcgatgtt gaaggactcc gccaccgtgc tcacgaactc
       61 ccgctcctgc tcctccacct tgccgttggc atggatcagc tccaacagat ggaacaggac
      121 cacgaccttc tggcgctgcg tgagttcctc attcacctgc gcgcagatcc gcagcacctt
      181 gacactgttc aagctggtcc gtttgcggcc cttgtcgctc gtgcctccgc tggtgtggaa
      241 tgcctccacg aacgcatcga atgtggcgat gtgcatggcc gcctgctccg gcgtgaactg
      301 ttgcagcagg aatcgcgaaa ccgtgcgttc tcccctgcgg ctggatatac caccaccttc
      361 agccttcgcg atgatcgcga acaactggag cagggctttg aggatcttct cgctcatcta
      421 caggcatgcc ccgtccacgg ctcccttcgt gcatgcacgg gtcgggccgt aaaagagcag
      481 ccgttcttac gacaggaacg agcgaaagtt gccgtgcggc gcgcagggca gcgaatgccc
      541 tggggcctgc cgcgagaagc cggacaatac gttcctccta ctttttgaat ccgatcagca
      601 tcaccgcaca accactcgcg gccttgctgc ggtccaattg cgcttcgatg gtgacgtcga
      661 tggtgttgct caccaccagg tcccagtacg gatcgttgtt ctggtcgcgg ttggtgtaca
      721 acacgttgcg atcacgatcg agtaccgaga agatcagatt gccgtcgctc agcccactgc
      781 acgcggccac acggtacgtg gttccaccat agagggtgag gtcgaattcg gccatttcat
      841 cctcacgcag cagtgcgcgg tagaattggc catcagggat gtacgcggcg gtgatgtgcc
      901 gctcgcagat actcgcgatg gtatcgcact gggccgaagc tgccgagccg agcaggatgc
      961 cgctggcaag caggaaaaac cgggtcgtct tgcgcatggt tcaggcggtg agggttgcgc
     1021 ggagggcctt caccttctcg gcgatggcct tgaactgctc ggcggtgatg gtcacggagg
     1081 aaacactgtt gatccaggtg gtcttcttcg ccacatcggt cgtggccgga cggaattcgt
     1141 acgtgctggt cacgccctgg tacgcggcaa gcacgtcttt caggccggtg atcaagctga
     1201 ccacgggttg ttcggtcccg cctcccgctt cgagcaatga tatgagattc tcgatcgttt
     1261 cgcgttgttc gccgaggcgt gtgttcagcg tcgcatcggt tttctccgtg gtcccgctga
     1321 cggttaggaa catgccctcg atccagccac ccgcgagcac caatgcgctc acatcatcgc
     1381 gcttgttgtt cttcaggtac tggtcggctg aacggaaggc cttgccggta aagcgcagca
     1441 gactgtcctc gttgttcaac cccttcttga acccgtcgag caattcctga tcgaatgcgt
     1501 tggagagatt caggtcggag ctcaacttct gcacggtctg cagggtggtg agggcacgct
     1561 gaccgtcctt gtggacagta aggtaggcca ggtcggctcc gtacgcaccg agtgccaagg
     1621 ccttttgaac gttgccggcc aggttggcag cggtggtcac aggcatcggc aacgccttgt
     1681 cgtagggcag tcccaccttg cggatgagca gggctgtctg cacaggcgat gggatgctga
     1741 agagttcgcc accaaccttc atcaggccac ctccgctgcc ctgcgcggtg gtgtccaatg
     1801 tgtcggcctt cgccggggtg tccttggcac
//
LOCUS       DAOQOC010000677         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45506_length_1829_cov_5.717024, whole genome shotgun sequence.
ACCESSION   DAOQOC010000677 DAOQOC010000000
VERSION     DAOQOC010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45506_length_1829_cov_5.717024"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..>1829)
                     /locus_tag="PL106_10670"
     CDS             complement(158..>1829)
                     /locus_tag="PL106_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33572.1"
                     /translation="QIDNNLQWFISLNALDKTNGYISGKTNVVVYPAVQCQNVTATLD
                     ITGNTDVTPAQVSTSFVGCPPVSLSLDQSHFTCADLGTNTVTLTAVANSVTTTCNATV
                     TVVSADADGDLTLDCFDGCPLDPAKIAPGICGCGNVDTDLDNDLVCDADEDEPNMQLG
                     VVLAPGSQLELRLLPDHFFNDLVSATVITVRWLTTPGVSITGASAAYVDPAYTPAVGA
                     LQYGGTTTDGPYSYATFFTFGNGSLFDEGISWTANTEVPFFRVPYVNNTLSCVTFEVM
                     DDTYQIDNNLQWFISLNALDKTDGYIPGKTSVCADPSSVMITSKVILEGGWQSGPSLM
                     RDDLRVLANFPLAHPYGSAPFNHSGTETTTPSVLAAAGSNAIVDWVLLELRDPLTPAL
                     VLASRAALLQRDGDIVDVNGIDPVTFNGTTPGNYYLAVRHRNHLGIMTTSALAMSPMT
                     PMIDFTLGSTPTYGTDARTTLGPVSAMWCGNVEPDDQLKYTGSTNDRDPILVAIGAIP
                     TATVTDYLPTDVTLDGVVKYTGAGNDRDPILVNIGGSVPTAIRPEQLP"
BASE COUNT          335 a          509 c          611 g          374 t
ORIGIN      
        1 cccaagagta tcattcccat cgtcaggatg ccgatcgagc gtacgtgacg ccagaacgtg
       61 gtgatcgttc caataccatc ccacatcaat tgatgtgttc gatcggtgag tcggagctgg
      121 gccatgggtc agaaggtaag ggttcgggta agaggggtca gggaagttgc tcgggtcgga
      181 tcgcggtcgg gaccgagcca ccgatgttca caaggatcgg gtcccgatca ttgccggcac
      241 ctgtgtactt cacaacgccg tccagtgtca cgtccgtagg gagatagtcg gtcaccgttg
      301 ccgtggggat cgccccgatg gccaccagga tcggatcccg gtcgttggtg gagcctgtat
      361 acttcagttg gtcatccggc tccacgttgc cgcaccacat cgcactcacc ggtcccaagg
      421 tcgtacgggc atcggtgccg tacgttggcg tgctgcccaa ggtgaagtcg atcatcggtg
      481 tcatgggact catggccagt gccgatgtcg tcatgatgcc gaggtggttg cgatgacgaa
      541 cggccaggta gtaattgccc ggggtggtcc cgttgaaggt gaccggatcg atgccgttca
      601 catcaacgat gtcgccgtcg cgctgcagca gcgctgcgcg actggccaga acgagggcag
      661 gtgtcaatgg atcccgaagt tccagcagta cccagtcgac aatggcgttg ctgccggcag
      721 cggcaagcac cgagggtgtg gtggtttcgg tgccgctgtg attgaagggg gcggaaccat
      781 agggatgggc cagcgggaag ttcgcgagca ccctcaggtc gtcccgcatc agcgacggtc
      841 cggattgcca gccaccttcc aggatgacct tggaggtgat catcacggat gaaggatccg
      901 cgcacaccga tgttttgccc ggaatgtacc cgtccgtctt gtccagcgcg ttcaggctga
      961 tgaaccactg caggttgttg tcgatctggt aggtgtcgtc catcacctcg aaggtgacgc
     1021 acgacaaggt gttgttcacg tacggtacgc ggaagaaggg cacctcggtg ttggcggtcc
     1081 agctgatgcc ctcgtcgaag agcgatccgt tgccgaacgt gaagaaggtg gcgtagctgt
     1141 acggtccgtc ggtcgtggtg ccgccgtatt gcagggcgcc cacggcaggc gtgtaggccg
     1201 ggtccacata cgccgctgag gcacctgtga tgctcacgcc gggcgtggtc aaccagcgta
     1261 ccgtgatcac ggtagcgctc accaggtcat tgaagaaatg gtccggcaat aagcgcagtt
     1321 ccagctggct gcctggtgcc agtacgaccc cgagctgcat gttgggctca tcctcgtccg
     1381 catcgcagac caggtcgttg tccaggtcgg tgtccacgtt gccgcacccg cagatgccgg
     1441 gagcgatctt ggccggatcc agcgggcaac catcgaagca gtccagggtg aggtcgccat
     1501 cggcatccgc ggaaacgacg gttaccgtgg cgttgcaggt ggtggtcacg ctgttggcca
     1561 cggccgtgag ggtcacggtg ttcgtaccga ggtcggcgca ggtgaagtgc gactggtcaa
     1621 gtgagaggct tacgggagga caacccacga aactggtgct cacttgtgca ggggtcacat
     1681 ccgtgttgcc ggtgatgtcc agcgtggccg tcacgttctg gcattggacc gcagggtaca
     1741 ccaccacatt ggtcttgccg ctgatgtagc cgttcgtctt gtccagcgcg ttcaggctga
     1801 tgaaccactg caggttgttg tcgatctgg
//
LOCUS       DAOQOC010000678         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45531_length_1829_cov_2.861894, whole genome shotgun sequence.
ACCESSION   DAOQOC010000678 DAOQOC010000000
VERSION     DAOQOC010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45531_length_1829_cov_2.861894"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PL106_10675"
     CDS             complement(<1..207)
                     /locus_tag="PL106_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33573.1"
                     /translation="MKVILFTRSLNAGGTERQMVLLAHGLAARGHEVLVVLLYGGGAL
                     ERTLDRRAVRLATLDKKGRWNVLGP"
     gene            complement(351..1322)
                     /locus_tag="PL106_10680"
                     /pseudo
     CDS             complement(351..1322)
                     /locus_tag="PL106_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
     gene            1428..>1829
                     /locus_tag="PL106_10685"
     CDS             1428..>1829
                     /locus_tag="PL106_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33574.1"
                     /translation="MADTARPQRFAPFWRVLATLGVVAMLFSAQRALFWVLNRDDFPH
                     PPLSAFLGGLRFDASAIAWLFLPWCLAMLIRPATHGAWHRVQLVLFHISTVFCFFLNC
                     TDLEYYKFTLKRSTVDLLGIAGGGSDLAHLAP"
BASE COUNT          276 a          657 c          529 g          367 t
ORIGIN      
        1 cggccctagc acgttccagc gccccttctt gtcgagcgtc gcgagccgaa ccgcacggcg
       61 gtcaagcgtc cgctccagcg caccgccgcc atagaggagc acgaccagca cctcgtgccc
      121 gcgggctgcc aggccatgcg ccagcagcac catctggcgc tcggtcccgc ccgcgttcag
      181 cgaccgcgtg aacaggatca ccttcatgcg gccaagccat agaggcggag cgtctcgtcg
      241 atcatgcgcg cttcgcgacc accgggatcc ggttccagcg gcaatggcga ccgtgccgcc
      301 tgtcctgcat cggacctggc gtacacatcg ccctcctcca cctccttcac ggccaccgga
      361 tccggctcct cgaaggcatg cttcaggtcc tcctcgatac cgtccttcag gcgcgtgcgg
      421 atgctgtcgc gcgaagcgat gctcaggatg ccctgccgca acagccactc ctcgtagttg
      481 gcaacgggat cttttctacc ccattcctca aatagttcct tgggcacgta cttcgttccg
      541 ctggcctcct catgcccgcg catgcggaag gtgatgcact ccaccaggat gggacgcggg
      601 ttctcgcgca cactgtccgc cagcctcgcc agggtgtcat acacctcgag gatgttgttg
      661 cccgccacct gcacgccttc gatgccgtac ccgaccgcct tgtccacgaa gctcttcatg
      721 cggaactgct cgtggctggg cgtgctcagc ccgtatccgt tgttctcgat gacgaagagc
      781 acgggaaggt cccacaccgc cgcaacgttc aggctctcgt ggaagtcgcc ctcgctggtg
      841 gccccatcgc ccgtgaacac gatgacgcac cgatcctcct tcttcagttt gtgcgccagt
      901 gcgatgccat cggcgatgcc catctggggt cccaggtggc tgatcatgcc caccaccttg
      961 tgctcctggt cgccgaagtg gaagctgcgc tcgcggccct tgctgtagcc ggtggccttg
     1021 ccctgccact gggcgaacag cttgcggaac ggcatgccac gcgccgtgaa cacgcccagg
     1081 ttgcggtgca tgggcaggat gtactcgtcg ttgcgcagcg ccatggccgc acctacggcg
     1141 atggcctcct gcccgatgcc gctgaaccat ttgctgatcc tgccttgccg cagcaggctc
     1201 agcatcttct cttcgatgat gcgcgggtat accagcttct cgtaggtgtc gatcaggaag
     1261 gcatcgctat gcgcgccgcg atcgaaggcg agctgtttct gctcggtggg gtcgatgatc
     1321 atggggtgaa cgggcggcaa aggtatcccg tgcgtgcacc gtggaacgca tcggcctcta
     1381 tcctccagaa catcccgacc cggcgttcag taggttcgcg cacccgcatg gccgataccg
     1441 cccgcccgca acgtttcgca ccattctggc gcgtgctggc caccctgggc gttgtggcca
     1501 tgctcttctc agcgcagcgc gcgctgttct gggtgctcaa tcgggacgac tttccccacc
     1561 cgcctctcag cgccttcctg ggcggcctgc gcttcgacgc cagtgccatc gcctggctct
     1621 ttctgccctg gtgtctggcc atgctcatcc gtcccgccac gcacggggcc tggcaccggg
     1681 tgcaactcgt gctcttccac atcagcacgg tcttctgctt cttcctcaac tgtaccgacc
     1741 tggagtacta caagttcacc ttgaagcgca gcacggtcga tctgctcggc atcgccggtg
     1801 gtggcagcga cctggcccac ctggccccg
//
LOCUS       DAOQOC010000679         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45619_length_1827_cov_3.430587, whole genome shotgun sequence.
ACCESSION   DAOQOC010000679 DAOQOC010000000
VERSION     DAOQOC010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45619_length_1827_cov_3.430587"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PL106_10690"
     CDS             complement(<1..1821)
                     /locus_tag="PL106_10690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA33575.1"
                     /translation="MNDELMKEAFDRSQWEIRASHMLAQLNSDPTPADTLAAWKKINV
                     MRDRVMKGEDWATVAAGKGGSDDPSVAKNGGDLGWFSALQMVYPFETAAFNTPKGEIS
                     KPVRTRFGYHIIKVVDKRPARGQVKVAHIMIRSTDQDSPEKQKAAEAKIQEVAGRLTS
                     GELSFSDAALKYSDDESTNTKGGELPMFGTGKMIDEFENVAFGLKNDGEVSPPFRTRY
                     GWHIIKRLEYKAPPSYEEAKADLKAKIARDSRAEITRNKFLTDLKAEYKLKQDPKALS
                     ALYALVDSTIFKKGVTINDTLLRKDVVEGPIMHKGQRYRREINGTMQAGKVINVRSRK
                     HDELSRSENDTVVVRDVHEGWSYDRTKAKALTKPLFSITGKTWTQRDFLDYLEKAQKR
                     ENPVPVQQYVKERFAKYVDDEVLAYEDSQLETKYPEFRMLMKEYRDGILLFELTDQRV
                     WSKAVKDSTGLAAYHEAHKADWMYPDRYELDIYTCKDAGVAGQVRKLYGKGKRGVLLT
                     EAINKPDQNAVVDVESGIFTEEEKPLIKGVTSPGLLADQKLDGGKVVITDLKRVVPPA
                     PKPLNEVRGLVTAAYQDQLEKDWIKELRAKYPVRVEQDVLY"
BASE COUNT          273 a          600 c          513 g          441 t
ORIGIN      
        1 gtacagcacg tcctgttcca cccgtaccgg gtacttcgcg cgcagttcct tgatccaatc
       61 cttctccagc tggtcctggt aggcggcggt caccagcccg cgcacctcgt tcaacggctt
      121 cggggcgggc gggaccacac gtttcaggtc ggtgatgacg accttgcccc catccagttt
      181 ctgatccgcc aggaggcccg ggctcgtaac acctttgatt agcggtttct cctcctcggt
      241 gaagatgccg ctctccacgt ccaccaccgc gttctgatcc ggcttgttga tggcctcggt
      301 aagcaggacg ccacgcttgc ccttgccgta cagcttgcgc acctgccccg ccaccccggc
      361 atccttgcac gtgtaaatgt ccaactcgta gcggtcggga tacatccagt ccgccttgtg
      421 ggcctcgtgg tacgcggcca gcccggtgct gtccttcacc gctttgctcc acactctctg
      481 atcggtgagc tcaaaaagca ggatgccatc ccggtattcc ttcatcagca tgcggaactc
      541 aggatacttc gtctcgagtt ggctgtcctc gtacgccagg acctcatcgt ccacatactt
      601 cgcgaagcgc tccttcacat actgctgcac cggtacgggg ttctcacgct tctgggcctt
      661 ctccagatag tcgaggaagt cgcgctgggt ccaggtcttg ccggtgatgc tgaacagggg
      721 tttcgtgagg gccttggcct tggtccggtc gtagctccag ccttcatgca catcgcgcac
      781 cacgaccgta tcattctcgg acctgctcag ttcatcgtgc ttgcggctgc gcacattgat
      841 gaccttgcca gcctgcatgg taccattgat ctcgcgcctg taccgctggc ctttgtgcat
      901 gatgggacct tcgaccacgt ccttgcgcag cagcgtgtcg ttgatggtga cacccttttt
      961 gaagatggtg ctgtccacca gggcatacaa cgccgagagg gccttggggt cctgcttcag
     1021 cttgtactcg gccttcaggt cggtgaggaa cttgttacgc gtgatctcgg cacggctatc
     1081 acgggcgatc ttggccttga ggtccgcctt ggcctcctcg taggagggtg gggctttgta
     1141 ctccagccgc ttgatgatgt gccatccgta acgcgtgcgg aacggcgggc tcacctcccc
     1201 gtcgttcttg aggccgaagg cgacgttctc gaattcgtcg atcatcttgc cggtaccgaa
     1261 catgggcagc tcaccgccct tggtgttcgt gctctcgtcg tcactgtact tgagtgcggc
     1321 atcgctgaag ctgagttcgc cactggtcag tcgccccgcc acttcctgga tcttcgcctc
     1381 ggcggccttc tgcttctccg ggctgtcctg gtcggtgctg cggatcatga tgtgcgccac
     1441 cttcacctgg ccacgtgccg ggcgcttgtc caccactttg atgatgtggt acccgaagcg
     1501 cgtacgcacg ggcttgctga tctcgccctt cggggtattg aaggccgcgg tctcgaacgg
     1561 gtacaccatt tggagtgcac tgaaccagcc caggtcgcct ccgttcttcg ccacgctcgg
     1621 atcatcgctg ccccctttcc ctgcggcaac ggtcgcccag tcctcccctt tcatcacacg
     1681 atcgcgcatg acattgatct tcttccaggc ggcaagggta tcggccgggg tgggatccga
     1741 gttcaactgc gccaacatgt ggctggcccg gatctcccac tggctgcggt cgaaggcctc
     1801 cttcatcaac tcgtcgttca agttgcg
//
LOCUS       DAOQOC010000680         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45658_length_1826_cov_4.435347, whole genome shotgun sequence.
ACCESSION   DAOQOC010000680 DAOQOC010000000
VERSION     DAOQOC010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45658_length_1826_cov_4.435347"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PL106_10695"
     CDS             complement(<1..656)
                     /locus_tag="PL106_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNA33576.1"
                     /translation="MKQLSIALSIAIALPCIMRAQGVDDARLKELRDRYDALQAQQDS
                     LLGPIEDAKLAIMQRDLAATGLPALAPGDVVTVHPGHSLVWDETHHVPKWTAHIVMPD
                     IMKGNLARIDTFKPDPEVKGHTDLFKEYWNSGYDRGHMVPSADMRWSRKALEATYLYS
                     NIAPQKAEMNREAWADLEDWGRRYVHYAQERVFIVTGPVITPDLPVLSTPTAIQKVTI
                     P"
     gene            1019..1342
                     /locus_tag="PL106_10700"
     CDS             1019..1342
                     /locus_tag="PL106_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33577.1"
                     /translation="MKSNTDIRFFKRMLLCNKCGNTWLTAEVPEKFINELVELRDALR
                     DIKANAGSYLDGSKKSAASSTELRESHSVLQALKIHGHQAWNIPFRSGRSYAHRIQAM
                     RAPWR"
     gene            complement(1320..1649)
                     /locus_tag="PL106_10705"
     CDS             complement(1320..1649)
                     /locus_tag="PL106_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA33578.1"
                     /translation="MKYEVRPLSVFERKARRLIGKYPSLKRELAALGASLEEEPRQGT
                     ALGKDCYKIRLAIVSKGKGKSGGARVITNVVVRDRTVHLLTIYDKSEQADLSAKELDA
                     LIAKVPA"
     gene            complement(1639..>1826)
                     /locus_tag="PL106_10710"
     CDS             complement(1639..>1826)
                     /locus_tag="PL106_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33579.1"
                     /translation="EKSKAGLLVEFLRSLPYVKVKAGAEEGSSLAAELEEAVEELKLV
                     RAGKKKTRTLKAFLDEV"
BASE COUNT          363 a          534 c          531 g          398 t
ORIGIN      
        1 gggatggtca ctttttgtat ggcggtcggg gtgctcagga cgggcaggtc tggcgtgatc
       61 accggtccgg taacgatgaa cacgcgctcc tgggcgtaat gcacatagcg ccgaccccag
      121 tcctcgaggt cggcccaggc ctcgcggttc atttcagctt tctgcggggc gatgttgctg
      181 tacagatagg tggcctcgag cgccttccgg ctccagcgca tgtcggcact cggcaccatg
      241 tgcccgcgat catacccgct gttccagtat tctttaaaaa gatcggtatg gcctttcacc
      301 tccgggtcgg gcttgaaggt gtcgatgcgc gcgaggttgc ccttcattat gtcgggcatc
      361 acgatgtgcg ccgtccactt gggcacgtga tgggtctcgt cccaaacgag ggagtggccg
      421 ggatgcaccg tcaccacgtc acccggggcc agtgcgggca gcccggtggc ggccagatcg
      481 cgttgcatga tggcgagctt ggcgtcctcg atgggaccga gcagggaatc ctgttgtgcc
      541 tggagggcat cgtatcggtc gcggagttcc ttcaaacggg cgtcatccac gccctgtgcc
      601 cgcatgatgc acgggagtgc gatggcgatg gacagggcga tcgatagctg cttcatggcc
      661 gcgcgaatgt aggtcgggtg cttgtagagg gatcaggaac ctgtggtgcc ctgtccgggg
      721 agcacaacca tacacttgcg gccaacttca gcccgatccg gggcggagga atctgtccat
      781 tccacacgta ttcggcccgg acaggaatga tggggccgag gcatgtgtgg tccttgagca
      841 gatacgaata ctcatcttcg gaacactcct cctattcgca gaggaaggtc caaattcatt
      901 cacatcaacg caaatgaacg gaggaacgaa aatatggtgc ccataatgca agaagatcac
      961 agtttgctca gcatgggatc cttcggggct tactggggta tccgggcaac gatcctacat
     1021 gaagagcaat actgatattc gatttttcaa gagaatgctg ctgtgcaata aatgcggcaa
     1081 cacatggttg acagccgaag tgccggaaaa attcattaat gagctggtcg aactgcgaga
     1141 tgctctgcgc gacatcaagg ccaatgctgg atcatatctg gacggatcga aaaagtctgc
     1201 tgcgagttcg accgagcttc gcgagtccca tagcgtgctg caagcgttga agatccatgg
     1261 acatcaggca tggaacatcc cgttcaggag tggacgatca tacgcgcacc gaattcaagc
     1321 tatgcgggca ccttggcgat gagtgcgtcc agctccttgg cgctcaggtc ggcctgttcg
     1381 ctcttgtcgt agatggtgag cagatggacc gtgcggtcac gtaccaccac gttcgtgatc
     1441 acacgagcgc cgccgctctt gcctttcccc ttgctcacga tcgctaacct gatcttgtag
     1501 caatccttgc caagggccgt gccctgcctc ggttcctctt ccaaggatgc accgagcgcc
     1561 gcgagttccc tcttcaggga tggatacttg ccgatcagtc tccgggcctt ccgttcgaaa
     1621 acgctgagcg gcctgacctc atacttcatc gaggaacgcc ttcagcgtac gggtcttctt
     1681 cttaccggcc cgcacgagct tcaattcctc caccgcctct tccaactccg ccgccagcga
     1741 cgacccctct tcggctccgg ccttcacctt cacatacgga agactccgca ggaactccac
     1801 gaggagtccc gccttgctct tctcca
//
LOCUS       DAOQOC010000681         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45786_length_1824_cov_3.182589, whole genome shotgun sequence.
ACCESSION   DAOQOC010000681 DAOQOC010000000
VERSION     DAOQOC010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45786_length_1824_cov_3.182589"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1161
                     /locus_tag="PL106_10715"
     CDS             145..1161
                     /locus_tag="PL106_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33580.1"
                     /translation="MYFTTNSTAFYDTNMSYDNTGRLWIAATLEYSMIVDGDTLEVSG
                     PDQYDHAIIEFAPDGGLLAHTIVPYEDQPARGMRCDHLIPHPDGGVLWMGTYLNGTTL
                     NGTVLDSAGNGSNALIMRLDSLLEMQWHTVFGADSSEAVTVGCIDGSTGKIHLAGIAG
                     NGTVIGPDTFPSDPVWFGFLMTMDSDGMIENGQFYCGSTVPWSGSPYELLCVGGQCHI
                     LGTTGMFELFTDGTLLQNAHYLARFDAEGTTSITTAARSNASFQIIPCPSQGPVDLRL
                     HPSHDGRYNVSVMNALVETVRQEVLFFIAGTAHLDLSGLAGGIYTIHLNTAEGPVVCR
                     TIIE"
     gene            1180..1386
                     /locus_tag="PL106_10720"
     CDS             1180..1386
                     /locus_tag="PL106_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33581.1"
                     /translation="MALRKAFTLSMTPLLLSNMARWSVMRGVDLWVRYGAWIYNDVTS
                     ISSGLQETSGSRKSDVKVEVRVRL"
     gene            complement(1557..>1824)
                     /locus_tag="PL106_10725"
     CDS             complement(1557..>1824)
                     /locus_tag="PL106_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNA33582.1"
                     /translation="EAMDRGIMGKVFVEFVVEKDGHVDSVRVKRGVHALLDAEAVRVV
                     GSMKNWIPGMQAGEVVRSRYVLPISFVMQEKDVQRARKRAKRKG"
BASE COUNT          374 a          569 c          537 g          344 t
ORIGIN      
        1 agcagcggga tgtggcccag gccccggtgg gccgtggtcc ggacccactg aaacacattg
       61 gccatcagcc ccgccaggag gccgaggacc agcgcataac cgatctcctt cgcgcccagc
      121 ggcgatctgg aatggcaaaa gccattgtac ttcacgacca atagcacggc gttctacgat
      181 acgaacatgt cctacgacaa taccggacgg ctatggatcg cggcgacgct ggagtacagc
      241 atgatcgtgg acggtgatac actggaagta tcaggccccg accagtacga ccacgcgatc
      301 atcgaattcg ctccggatgg cggtttgttg gcacacacca tcgtccccta cgaggaccag
      361 cccgcccggg gcatgcgctg cgatcatctg ataccccacc cggacggtgg cgttctatgg
      421 atggggacct atctgaacgg cacaacgctg aacggaaccg tgctggacag cgcgggcaac
      481 gggagtaacg cgctcatcat gcggctcgac agcctgttgg agatgcaatg gcatacggta
      541 ttcggagcgg actccagcga ggctgtgacc gtcgggtgca tagatggatc cactggaaag
      601 atccatcttg caggaatcgc cgggaacgga acggtcatcg gtccggacac ctttccgagc
      661 gaccccgtct ggttcggatt cttgatgacg atggactccg atggaatgat agagaacggg
      721 caattctatt gtggttcgac ggtgccctgg tcaggttcac catacgagct cctgtgcgtt
      781 ggaggccaat gccatattct gggtaccaca gggatgttcg agctcttcac cgatggtacg
      841 ctgctgcaga atgcccacta ccttgctcgc ttcgatgccg aaggcaccac atccatcacc
      901 acggcagccc gctcaaacgc gtcgttccag atcataccct gcccatcgca gggtccggtg
      961 gaccttcggc tccacccatc gcacgatggc cggtacaacg tttctgtgat gaacgcgtta
     1021 gtcgaaacgg tgcggcagga ggtgctgttc ttcatcgccg ggactgcaca cctggacctg
     1081 tccggtcttg ctggtggcat atataccatc cacctcaaca ctgcagaggg cccagtggtg
     1141 tgtcggacga tcatcgaatg acctcctgat catcgcgcaa tggctctccg caaagcgttc
     1201 acattgtcca tgacgccgct tctcctgagt aacatggccc gctggagcgt gatgcgcggc
     1261 gtggacctgt gggtgcgcta cggcgcctgg atctacaatg atgtcaccag catcagcagc
     1321 ggcctgcagg agaccagcgg gagccggaag agcgatgtga aggtggaggt gcgggtgcgg
     1381 ttgtgaggga gtcagccccc gcgccatacg tccggaggag cggaaaggca ggtcacgctg
     1441 agtccgtcaa agcacgagcc cgccggaccg tgacattcac cggacctgga acggaggtac
     1501 atgcgcatgg gcgatccagg gcgcatgccg gacaaggcga tcgacattcg ccgtgctcaa
     1561 cccttgcgct tcgccctctt tctggcccgc tgcacatcct tctcctgcat gacgaaactg
     1621 atgggcagta cataacggct gcgcaccact tcgccggcct gcatgcccgg tatccagttc
     1681 ttcatgctgc ccaccacgcg cacggcttcg gcatcaagca gggcatgtac gccgcgtttc
     1741 acccgcacgg aatccacatg gccgtccttc tccaccacga actcgacgaa gaccttgccc
     1801 atgatgcccc ggtccatcgc ctcc
//
LOCUS       DAOQOC010000682         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45836_length_1823_cov_3.356900, whole genome shotgun sequence.
ACCESSION   DAOQOC010000682 DAOQOC010000000
VERSION     DAOQOC010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45836_length_1823_cov_3.356900"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PL106_10730"
     CDS             <1..1078
                     /locus_tag="PL106_10730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNA33583.1"
                     /translation="DVYVHPSSGAMMAYLTSDRIDPERGVWVDELATFDPVRDKRGRV
                     VALVHASEPLRGIMDTAKARLWRDIGLATVLLTVIAAFLLRSVGRIVEREEAAHGQLR
                     KRHEGITDSIAYAGKIQGALVPDAGVYAEMFDDHFVLNRPKDTVSGDFHWYHRIGEDE
                     CFVAAADCTGHGLPGAMMAAIGCSLLNEIVSTHPHKDPAELLALLNERMVHTLHQSGR
                     RMGAGDGMDVALCRIDRRKRELLFAGAFRPLYWMHEGQISVINGDRKPIGGSQHGDHR
                     KYTVHRVAYHSGDRIYLFSDGYVDQFGGPERKRFMSTRFSQMLAEHQHLPMALQAEVL
                     DRAFLDWKGVNEQVDDVCVLGIAV"
     gene            complement(1138..1341)
                     /locus_tag="PL106_10735"
     CDS             complement(1138..1341)
                     /locus_tag="PL106_10735"
                     /inference="COORDINATES: protein motif:HMM:NF025189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HNA33584.1"
                     /translation="MLTGNYLFAGSDRRARHAALMCSLLGTCKLHGMEPFAYLSDVIA
                     RISDHKANKLYELLPQNWQPLAK"
     gene            complement(1397..>1823)
                     /locus_tag="PL106_10740"
     CDS             complement(1397..>1823)
                     /locus_tag="PL106_10740"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA33585.1"
                     /translation="FHGTLQTDGYAGYEKLAARLERPPLGGGSGGAALACMAHARRYF
                     DKALSSDKVRAEHALRLISELYTIERRCDEKKALPELQHRYRRRRALPLLDDLEKWLK
                     EEIIMIAPKSPLRAGHRLLPIALDAPTCLYRRRPIPDRQ"
BASE COUNT          332 a          560 c          590 g          341 t
ORIGIN      
        1 cgatgtgtac gtccatccct ccagcggggc catgatggcc tatctcacct cggatcgcat
       61 cgatcccgaa cgtggagtat gggtcgatga attggcgacc ttcgatcccg tacgcgacaa
      121 gcgcggacgc gtagtggcgc tggtgcatgc ctccgagccg ttgcggggca tcatggacac
      181 ggccaaggcc cgcctgtggc gcgacatcgg cttggccacg gtcctgctca ccgtgatcgc
      241 ggccttcctg ctgcgtagcg tgggccgcat tgtagagcgc gaagaagcgg cccatggcca
      301 gttgaggaaa cggcacgaag gcatcaccga cagcatcgcc tacgcgggca agatccaagg
      361 cgccctggtg cccgatgccg gtgtctacgc tgagatgttc gacgaccatt tcgtactcaa
      421 ccgcccgaag gacacggtga gtggcgactt ccactggtac catcgcatcg gcgaggacga
      481 gtgcttcgta gccgctgcgg actgcacggg gcatggcctg cctggcgcca tgatggccgc
      541 catcgggtgc tccctgctga acgagatcgt aagcacgcac ccgcacaagg accccgctga
      601 gttgctcgct ctcttgaacg agcggatggt acacaccctc caccagagcg gccggcgcat
      661 gggtgcgggc gacggcatgg atgtggcgct ctgtcgcatc gaccgaagaa agcgcgagtt
      721 gctcttcgca ggggccttcc gcccgctcta ctggatgcac gagggccaga tttccgtgat
      781 caacggcgac cgcaagccca tcggcggctc ccagcacggc gaccaccgga aatacaccgt
      841 acaccgggta gcctaccaca gcggtgaccg catctacctt ttcagcgatg gctacgtgga
      901 ccagttcggc ggacccgagc gcaagcgctt catgagcacg cgcttcagcc agatgctcgc
      961 cgaacatcaa cacctgccca tggccctaca ggccgaagtg ctcgatcggg ccttccttga
     1021 ctggaaaggc gtgaacgagc aggtggacga tgtgtgcgtg ctggggatcg cggtgtgatc
     1081 acccaccgac gttcgtgatc tgtaagcctc cggggaacta ccccttgcag aatcggacta
     1141 cttggctaac ggctgccagt tctgagggag gagctcatag agcttgttgg ccttgtggtc
     1201 gctgatccgc gcgatcacat cgctgaggta ggcgaagggt tccatgccgt gcagcttgca
     1261 ggtgccgagc agggagcaca tgagagcggc atggcgcgcg cgccggtcgc ttccggcgaa
     1321 gaggtagttc cccgttaaca caggacgaag acgcttgcgc ccgatggcca aggagcggat
     1381 ggtgttctct atgcggttat tgtcgatcag gtatcggccg tcggcggtat aggcacgtag
     1441 gcgcgtccag agcgatgggg agtaggcgat ggcctgcccg aagggggctc ttgggcgcga
     1501 tcattatgat ctcctccttc aaccatttct ccaggtcatc gagcaggggc aaggcgcgtc
     1561 gccttcgata gcgatgctgg agttccggca gcgctttctt ttcatcgcag cggcgctcga
     1621 tcgtgtacag ttcactgatc aacctcaggg cgtgttccgc gcggaccttg tcgctgctga
     1681 gggctttgtc gaagtagcgc cgtgcgtggg ccatgcacgc cagtgcagcc ccacccgacc
     1741 ctcccccaag gggagggcgt tccaggcgtg ccgcgagctt ctcgtaacct gcgtagccat
     1801 cggtctgcaa ggtgccatgg aag
//
LOCUS       DAOQOC010000683         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45885_length_1822_cov_3.089983, whole genome shotgun sequence.
ACCESSION   DAOQOC010000683 DAOQOC010000000
VERSION     DAOQOC010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45885_length_1822_cov_3.089983"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PL106_10745"
     CDS             complement(<1..369)
                     /locus_tag="PL106_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33586.1"
                     /translation="MRAAIAIPLVASLLTSCKKEEVMPEVPEIALVSMSTQNVTEFED
                     HVTVRFSYKDGDGDLGRTDPDDYTLWVKDARLENPDGYHIPPLAPEGSEVPIQGELEV
                     ELHALFLLGSSSEEQTHYTIH"
     gene            complement(366..1301)
                     /locus_tag="PL106_10750"
     CDS             complement(366..1301)
                     /locus_tag="PL106_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33587.1"
                     /translation="MRTAIHTTAALLLVLVACRKEENPFAQLEHRSPNPPSEALPHDN
                     FAWLHQRVFRPVCANSGCHDGTFEPEFRSIGSAYNSLVLAPVIANDPGETFTYRVVPG
                     DPAASFLHERLTVFVPNTSGMMPLETDGPDWPENHVQYIDAITSWIQSGAKDMFGNPP
                     TVGDLEPQVTGFLVFPHGSTNGAYPRGEGEGVQPIEVPATNVDLWFSFADDGTPASEL
                     GHNTMRIATSLLGFATVPELPLATDASMNGPDFGGSSTVFTHKGDLDLSGYAPGTLLF
                     VRVYVDDGEHDGPTGIPDDGTGAPMVDYFTLRITA"
     gene            complement(1303..>1822)
                     /locus_tag="PL106_10755"
     CDS             complement(1303..>1822)
                     /locus_tag="PL106_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33588.1"
                     /translation="TIAHPPVHDNGDAQTIGYACEVMKWFKPALTVVNMSAVDGCHAN
                     FTGYLASLHRADHSVGHLWNHIQTQIPEMAGNTAIVIAPECGRNDEPNAVRDQNDWLA
                     YDHSDQNSLRIFGLMAGAGVPSNLVVGGEDNPVGIATDIVPTVAELLGIKSEVMAAGL
                     IDYGSQSLFDRM"
BASE COUNT          306 a          531 c          622 g          363 t
ORIGIN      
        1 gtggatggtg taatgcgtct gctcttccga gctgctgccc agcaggaaga gcgcgtgcaa
       61 ctccacttcg agttcaccct ggatgggcac ttcgctgcct tccggggcga gcggtgggat
      121 gtgatagccg tcggggttct ccaggcgtgc gtccttcacc cagagcgtgt agtcatccgg
      181 atcggtccgc cccaggtcgc catcgccgtc cttgtaactg aagcggacgg tgacatgatc
      241 ctcgaattcg gtgacgttct gcgtgctcat gctcaccagg gcgatctcgg gcacctcggg
      301 catgacctcc tcctttttgc agctcgtgag gagcgatgcg accaatggga tggcgatggc
      361 ggccctcatg cggtgatcct taaggtgaag tagtccacca tgggcgctcc agtgccgtcg
      421 tccgggatcc cggtcggccc gtcgtgctcg ccatcatcca cgtagacacg cacgaacagc
      481 aaggtgcccg gggcgtatcc actgaggtcc aggtcaccct tgtgtgtgaa cacggtactg
      541 gaaccgccga agtccggtcc gttcatgctg gcatcggtgg ccagaggaag ctcgggcacg
      601 gtggcgaagc cgagcaacga ggtggcgata cgcatggtat tgtggccgag ttccgaggcc
      661 ggtgtaccgt catcggcgaa ggagaaccag aggtccacat tggtggcggg cacctcgatc
      721 ggttgaacgc cctcaccttc gccccgcgga tatgcgccgt tcgtggagcc gtgcgggaat
      781 acgagaaaac cggtaacctg tggttccaga tcgcccacgg tcggcgggtt gccgaacatg
      841 tccttcgcac cgctctggat ccagctggtg atggcgtcga tgtactggac atggttctcc
      901 ggccagtcgg gcccgtcggt ttccaagggc atcatgccgc tggtgttggg cacgaagacc
      961 gtcagccgct catggaggaa gctggcagcg ggatcgccgg gtaccacgcg ataggtgaag
     1021 gtctcccccg gatcattggc gatcaccgga gcgagaacca acgagttgta ggcgctgccg
     1081 atgctgcgga attcgggttc gaacgtgcca tcgtggcaac cgctgttggc gcaaaccggg
     1141 cggaagacac gctggtggag ccaggcgaaa ttgtcgtgtg gcagcgcctc gctcggtggg
     1201 ttcggcgagc ggtgctccag ttgcgcgaag gggttctcct ccttcctgca ggccacgagg
     1261 accagcagca acgcggcggt ggtgtggatc gcggtgcgca tgtcacatcc ggtcgaagag
     1321 actttgactg ccgtagtcga tgaggccggc ggccatcacc tcgcttttga tcccgagcag
     1381 ttcggccacg gtgggcacga tgtcggttgc gatgccgacg gggttgtcct cgccaccgac
     1441 cacgaggttc gagggcaccc ccgcaccggc catcaggccg aagatgcgca gggagttctg
     1501 gtcgctgtga tcataagcca gccaatcgtt ctggtcgcgc acggcgttgg gctcgtcgtt
     1561 gcgaccgcat tccggcgcga tgacgatggc ggtgttgccg gccatttcgg ggatctgggt
     1621 ctggatgtgg ttccagagat ggcccacgct atggtcggca cggtgcaaac tggcgaggta
     1681 tccggtgaag ttggcgtggc agccgtccac agcgctcatg ttcaccacgg tgagcgcggg
     1741 cttgaaccat ttcatcactt cgcacgcgta gccgatggtc tgcgcatcac cgttgtcgtg
     1801 aacgggcggg tgtgcgatgg tg
//
LOCUS       DAOQOC010000684         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_45925_length_1821_cov_3.806908, whole genome shotgun sequence.
ACCESSION   DAOQOC010000684 DAOQOC010000000
VERSION     DAOQOC010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45925_length_1821_cov_3.806908"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PL106_10760"
     CDS             <1..1203
                     /locus_tag="PL106_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA33589.1"
                     /translation="KEIEGLLDEAERVKKFIEEKNVPGVEALRIDIDRAKPEMPIVID
                     REKARRLNVSTYQVADAIRTAVFGKEVSTYRVEGDDDDYEINVRLKDAYRYDTQRIMD
                     MRITFRDMLTGQIRQVPISAVAKEEYRTTFSAIKRKDLDRVVQVSSNVIAGYNNNEVV
                     ATIKDEMASGFEASDRFNMRFTGEQEDQAKDQGFLGMAFLVAIFVVFLIIVAQFNSIS
                     YPVIVMSTVVFSTIGVLLGLVVFRMEFVGLMTTLGIISLIGVVVNNAIVLMDFARLLF
                     QRAQRKLGLDENTIIPIEESREALVIAGHTRLRPVLLTAVTAVLGLLPLAIGLNFNFF
                     TLFSELDPHIHMGGDNVVFWGPLSWAVIFGLIFATFLTLIMVPVMLLIVQKIKHRRQW
                     KRSLQAKG"
     gene            1630..>1821
                     /locus_tag="PL106_10765"
     CDS             1630..>1821
                     /locus_tag="PL106_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33590.1"
                     /translation="MSTSIERISRLLPITLRNVYRLIAVEMDKGGTELRLPQYGMLDF
                     LLTHSEAVQTDLANHFAKDK"
BASE COUNT          373 a          563 c          546 g          339 t
ORIGIN      
        1 aaggagatcg aagggctgct ggatgaggcg gaacgcgtga agaagttcat cgaggagaag
       61 aacgttccgg gcgtggaggc cctgcggatc gacatcgacc gcgcgaagcc cgagatgccc
      121 atcgtgatcg atcgcgagaa ggcgcggcgc ctcaatgtga gcacctacca ggtggccgat
      181 gccatccgca ccgcggtatt cggcaaggag gtgagcacct accgcgtgga aggcgacgac
      241 gatgactacg agatcaacgt gcgcctgaag gacgcctacc gctacgacac gcagcggatc
      301 atggacatgc gcatcacctt ccgcgacatg ctcaccggcc agatccgcca ggtgcccatc
      361 agcgccgtgg ccaaagagga gtaccgcaca acgttcagcg ccatcaagcg caaggacctg
      421 gaccgcgtgg tgcaggtgag cagcaacgtg atcgccggtt acaacaacaa cgaagtggtc
      481 gccacgatca aggacgagat ggccagcggc ttcgaggcca gcgaccggtt caacatgcgc
      541 ttcaccggcg aacaggagga ccaggcgaaa gaccagggct tcctgggcat ggcgttcctc
      601 gtggccatct tcgtggtgtt cctcatcatc gtggcccagt tcaacagcat cagctatccg
      661 gtgatcgtga tgagcaccgt ggtcttctcc accatcggcg tgctgctcgg cctggtggtc
      721 ttccgcatgg agttcgtggg cctgatgacc accttgggta tcatttcact catcggggtt
      781 gtggtcaaca acgcgatcgt gctcatggat ttcgcgcgcc tgctcttcca acgggcgcag
      841 cgcaagctcg gcctcgatga gaacacgatc atcccgatcg aggagagccg cgaggccctg
      901 gtgatcgccg ggcacacgcg gttacgcccc gtcctgctca ccgcggtaac cgccgtgctg
      961 ggcctgctgc cgctggcgat cggtctcaac ttcaatttct tcaccctgtt cagcgagctg
     1021 gacccgcaca tccacatggg cggcgacaac gtggtgttct ggggcccgct cagctgggcg
     1081 gtgatcttcg ggctcatctt cgccaccttc cttaccctca tcatggtacc ggtgatgctg
     1141 ctgatcgtgc agaagatcaa gcaccgcagg cagtggaagc gatcgctgca ggcgaaaggg
     1201 tgaggaagcc gcacggtcga ggaacgcctg agcggcctca tgaaagcagg ccccttcgcg
     1261 atgtgcggcc ttcctgtaag gtcttcgtca acttgcgcac gttggttgat gccgcagctt
     1321 ttccttcagc acacccgggc ggtgtacggt tgggtgccga ccacatgcgc gtataccggg
     1381 aacgcattgt gccctgatcc agaagcgatc acccgccatc ccgcccggta agggtctgcg
     1441 gtcgatctcg ggttcaaggg tgcgagagac gtgactctcg caccctcgca cttacgcacc
     1501 ccatggttca ttctgaccac gatgatcgag ggaccggatc ctcatcaccg gcccagtagg
     1561 tgtattcgca aatagttgca taacgaaaca attgatatat ttgcgccgtt aagaacagcg
     1621 atcattgcca tgagcacatc aattgagcgg atctcacgcc tgttgcccat caccctgcgc
     1681 aacgtctacc ggttgatcgc cgtggagatg gacaagggcg gcaccgagct ccgcctgccc
     1741 cagtacggca tgctcgactt cctgctcacc cacagcgaag ccgtccagac ggacctggcc
     1801 aatcacttcg cgaaggacaa g
//
LOCUS       DAOQOC010000685         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46025_length_1820_cov_1.611331, whole genome shotgun sequence.
ACCESSION   DAOQOC010000685 DAOQOC010000000
VERSION     DAOQOC010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46025_length_1820_cov_1.611331"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..615
                     /locus_tag="PL106_10770"
     CDS             121..615
                     /locus_tag="PL106_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA33591.1"
                     /translation="MATRRTIPALAPRKLPTQARAQRTYRAIVEAGARILERHGYEAL
                     TTNRVAELAGVGIASLYEYFPNKHALTAEVVRHVLDGLLADLGASTQLVTSAPPGSPE
                     AAMTAWLGAMYAAVAKRRAVVTVIVQEVPFLWAIPAVDHARTLRSIVGNCRAMAHVCE
                     TPFA"
     gene            complement(540..851)
                     /locus_tag="PL106_10775"
     CDS             complement(540..851)
                     /locus_tag="PL106_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33592.1"
                     /translation="MRAAKMRSRPSHPMFNGARILIAPLDWGLGHASRCIPIIRRLRE
                     LDARPIIGADTGALAVLRDAFPDLQHVRLPGVQVSYAKGVSQTWAMARQFPTMLRSVR
                     A"
     gene            850..1548
                     /gene="trmB"
                     /locus_tag="PL106_10780"
     CDS             850..1548
                     /gene="trmB"
                     /locus_tag="PL106_10780"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNA33593.1"
                     /translation="MGKGKQFKFEENATFAHVVQPPFEQVMSDAFPLKGTWNRDFFRR
                     DAPLILELGCGKGEYTTGLAQLRPGCNYIGVDIKGARIWRGARTAKELGLANVGFLRT
                     HVDHLLKVFGPGEVNGIWLTFSDPQLGKDRKKLTSPLFLERYRQVLAPGGVIHLKTDS
                     PELYHYTLDRVAQYGLPLYEHSADVYGELVPRLAPADQAMLNIRTFYESMWLGEGKKI
                     HYCRFGIHPLLGAG"
BASE COUNT          343 a          574 c          603 g          300 t
ORIGIN      
        1 cggggttcgc gtccgtgacg gatacgctgg cggtcgcgcg cgggaccggc ggcggccggg
       61 gccggttgca tggtacaacc ataatccgag caaaagctcg gttctacttg ggttcggacc
      121 atggccacca gacgcacgat cccggcgctg gctcctcgga agctgcccac gcaggcgcgg
      181 gcgcagcgca cctaccgcgc gatcgtcgag gccggcgctc ggatcctgga gcgccacggc
      241 tacgaggcgc tgaccaccaa ccgcgtcgcc gagctggccg gcgtcggcat cgcgtcgctg
      301 tacgagtact tccccaacaa gcacgcgctg accgccgagg tcgtgcgcca cgtgctcgac
      361 gggctgctcg ccgatctcgg cgccagcacg cagctcgtca ccagcgcgcc gcccggatcg
      421 cccgaggccg cgatgaccgc gtggctcggc gcgatgtacg ccgcggtggc gaagcgccgc
      481 gccgtggtca cggtgatcgt ccaggaggtg ccgttcctgt gggccatccc cgcggtcgat
      541 cacgcgcgca cgctccgcag catcgtcggg aactgccggg ccatggccca cgtctgcgaa
      601 acgcctttcg cgtagctcac ctgcacgccg ggcaggcgca catgctggag gtccgggaat
      661 gcatcgcgca acacggcgag cgcgccggtg tcggcgccga tgatgggccg cgcatccagt
      721 tcgcgcaggc gccggatgat cgggatacaa cgcgaagcgt ggccgagccc ccagtccaaa
      781 ggggcgatga ggatgcgcgc accgttgaac atcggatgcg aaggtcggga gcgcatcttt
      841 gcggccctca tgggcaaagg caaacagttc aagttcgagg agaacgccac gttcgcccac
      901 gttgttcagc cgcccttcga gcaggtgatg agtgatgcct tcccgctgaa aggaacatgg
      961 aaccgcgatt ttttccgtcg tgatgcaccg ttgatcctgg aactgggctg tggcaagggc
     1021 gaatacacca cgggcttggc gcaactacga cctggatgca actacatcgg tgtggatatc
     1081 aagggcgcgc ggatctggcg gggtgcgcgc accgccaaag agctcggcct ggccaacgtg
     1141 ggcttcctgc gcacgcacgt ggatcacttg ctcaaggtct tcggcccggg ggaggtgaat
     1201 gggatctggc tcaccttcag tgatccccag ttgggcaagg accgcaagaa gcttacctcg
     1261 cccttgttcc tggagcgtta tcggcaggtg ctggcacccg gaggagtcat ccacctgaag
     1321 acggacagcc ccgagttgta ccattataca ttggaccgtg tggcgcaata cggcttaccc
     1381 ctgtacgagc acagtgctga tgtgtacggg gaactggtgc cccggctggc cccagccgac
     1441 caggccatgc tgaacatccg taccttctat gagtccatgt ggctggggga ggggaagaag
     1501 atccactatt gccgattcgg gatacaccca ttgctgggag cggggtagaa caggctgtgg
     1561 ctccgttggg gtgaccccga agaacaggcc ccgtggccgt gaccggtcgc gccgggccat
     1621 accgcagaaa aacggtaccc ccgttgcggg gatcgggaaa ccgtataatt tcgccccgct
     1681 ttggcccgag gggttctttg gcgagaacac gtaaggagaa ggcaacaacc aaacacaacg
     1741 tcagtcgaac gcatgaacat ttacgtcgcc aacatcgcgt acagcgcgaa ggaccaggat
     1801 ctccgcgaac ttttcgaagc
//
LOCUS       DAOQOC010000686         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46156_length_1817_cov_3.826901, whole genome shotgun sequence.
ACCESSION   DAOQOC010000686 DAOQOC010000000
VERSION     DAOQOC010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46156_length_1817_cov_3.826901"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PL106_10785"
     CDS             <1..907
                     /locus_tag="PL106_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33594.1"
                     /translation="MCYRASAILCASALFLVSCGGNDAERATKDEPPALLIGGEDTAV
                     SAQQLYQMPTPNELFSIVRELGGGGHKNALSPVQKADRFATLPGRALNFGVYATDMVY
                     ASNFRLTSEVVRYYLTCRDLGDKLGLSSTFDAGILQRLEKNLTHGDSLDVLANDAYYN
                     AYQKLQTEQMGTTLALVLAGGWVESVHLVMAHVDAFDAASPLVARIAEQKASLEQLID
                     MMNTVQADPVTAPVLTRLEAIRDVYDQLAITRTSSPATSPSGRMTLGDAEQITISAEK
                     YQELKNAVAALRDTITKAEDASAKV"
     gene            933..1580
                     /locus_tag="PL106_10790"
     CDS             933..1580
                     /locus_tag="PL106_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33595.1"
                     /translation="MRSHVIIAAISALLALRSAAQDGCAGYHQFNCARATDVRFKING
                     QSKSASVQIGVPTELNIIVYKGQDYRISFCYDTKVIGDHVSARLVEKTRVPKEVTEQV
                     TRKEEIVDKDGNPTGQFKDVVGTEVKRVFEDVRKVLWNNEDHEMTDEVEFTATATKRI
                     VVEVIAPGSTEAKPKRSSKDPLDIGCVGILIEHMPTPNLGFEDQVKRTKLDEQAK"
BASE COUNT          396 a          536 c          567 g          318 t
ORIGIN      
        1 catgtgttac cgagccagtg ccatactctg cgcatccgcg ctatttctcg tgtcctgtgg
       61 cggcaacgac gcggagcggg cgaccaagga cgagccacca gcgttgttga tcggtggcga
      121 ggatacggcc gtgagcgccc agcagctcta tcagatgccc acgcccaatg aactcttcag
      181 catcgtgcgg gaattgggtg gtggcggtca caagaatgcg ctgagcccgg tgcagaaggc
      241 cgaccggttc gccaccttgc cgggtcgcgc gctcaatttc ggcgtgtacg ccacggacat
      301 ggtgtacgcc agcaatttca ggctcacgag cgaggtggtg cggtactacc tcacctgccg
      361 ggatctgggg gacaaattgg gtctttcctc caccttcgat gcgggcattc ttcagcggtt
      421 ggagaagaac ctgacccatg gcgattcgct ggacgtgctg gccaacgacg cgtactacaa
      481 cgcctatcag aagctgcaga ccgagcagat gggcacgacg ttggcgctgg tcctcgcggg
      541 tggctgggtg gagagcgttc acctggtcat ggcacacgtg gacgcgttcg atgccgcaag
      601 cccattggtc gcacggatcg ccgaacagaa agcgagcctg gagcaactca tcgatatgat
      661 gaacaccgtg caggccgatc ccgtaacagc cccggtgctc acgcgcctgg aggccatacg
      721 cgatgtgtac gatcaacttg ccatcacccg caccagcagt cctgccacct cgcccagcgg
      781 ccgtatgacc ttgggcgatg cggaacagat cacgatcagt gctgaaaaat accaggaatt
      841 gaagaacgcc gtggccgctc tccgggacac gatcacgaaa gcggaggatg cttccgccaa
      901 agtttgagga ttcagcgaag aagcccccat tcatgagaag tcacgtcatc atcgccgcca
      961 tttccgccct tctcgcgttg cgcagtgcag cgcaggacgg gtgcgcaggc tatcaccagt
     1021 tcaactgtgc ccgcgctact gacgtccggt tcaagatcaa cgggcagagc aagagcgcca
     1081 gcgtgcagat cggtgtgccc accgaactga acatcatcgt gtacaaggga caggattacc
     1141 gcatttcctt ctgttacgac accaaggtca tcggggacca cgtctccgcc cgtttggtgg
     1201 agaagacacg cgtgccgaaa gaagtgaccg agcaagtgac ccggaaggag gagatcgtgg
     1261 acaaggacgg caaccccacg ggccaattca aggatgtggt gggtaccgag gtcaaacggg
     1321 tgttcgagga tgttcggaag gtgctttgga acaacgagga ccatgaaatg acggatgagg
     1381 tggagttcac ggcaacggcc accaagcgga tcgtggtgga agtgatcgca ccgggctcca
     1441 ccgaggccaa gcccaaacgt tccagcaagg atccgttgga catcggttgc gtaggcatcc
     1501 tgatcgagca catgcccaca ccgaacctgg gtttcgagga ccaggtgaag cggaccaagc
     1561 tcgacgagca ggccaagtag tacacgtgcg gcgcaagtgt tcggcaggtg atccggcgtg
     1621 cgggaacgag cgctcgaccc cgttgggcat aagcggtcta ccttgccccc atgtacgttg
     1681 aagaccggag cgccccggct ccatggaaag cgggcctcgc cgcgctgcgc gagatcctgt
     1741 ccgcaccgcg caccatcgcc atcgtcacac atttcaatcc ggacggtgat gccatgggga
     1801 gcagcctcgg gctatgt
//
LOCUS       DAOQOC010000687         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46175_length_1817_cov_2.469921, whole genome shotgun sequence.
ACCESSION   DAOQOC010000687 DAOQOC010000000
VERSION     DAOQOC010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46175_length_1817_cov_2.469921"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /gene="nosZ"
                     /locus_tag="PL106_10795"
     CDS             complement(<1..1774)
                     /gene="nosZ"
                     /locus_tag="PL106_10795"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNA33596.1"
                     /translation="MKRPVLSALVLLSGAGGLFLLFNTMQGCRPQTAQTVVSGDVASK
                     VYIKPGSHDEFYNFVSGGFSGQMSVYGLPSGRLLRDIPVFSVDPENGWGYSEETKPML
                     MTTHGFIPWDDSHHVEASMTNGVPDGRYMFINGNNTPRVAMIDLKTFRTASIIEIPNS
                     SGNHSSPFITPNSEYLVAGTRFSIPMGTDAQRDVPIETYKENFKGTVSFIKPDTATST
                     MSIAFQITTPAVDFDLAHAGKGPSDGWFFFSCYNTEQAYSLLEVNASQRDKDFIMAVN
                     WKKAEQYAKEGKGKMIDGKHYMNHYDESVHSATSQVVDKVLQLDPKELTDLLYFMPCP
                     KSPHGCDVDPTGEFIVGSGKLAATLPVFSFTKMQKAIADKDFDGDFVGIPVLKYESVL
                     HGEVKKPGLGPLHTEFDNNGFAYSSMFVSSEIVKWNVKSLEVVDRVPTYYSIGHLCVP
                     GGDSKQPWGKYVIAYNKITKDRYLPTGPELTQSAQLFSIDGDKMELLLDFPTTGEPHY
                     AHAVPAELIKDKSTRFFKIEDNKHPYATKGEKEARVVREGNKVHVYMTCIRSHFAPDN
                     IEGVKVGDEVYFHVTNLEQDWDVPH"
BASE COUNT          322 a          507 c          566 g          422 t
ORIGIN      
        1 cgtgcggcac gtcccagtcc tgctccaggt tggtcacatg gaagtacacc tcgtctccca
       61 ccttcacacc ttcgatgttg tccggggcga agtgcgagcg gatacaggtc atgtacacgt
      121 gcaccttgtt gccttcgcgc accacgcggg cttctttctc gcccttggtg gcgtaagggt
      181 gcttgttatc ctcgatcttg aagaaccggg tggatttgtc cttgatcaat tcggcgggca
      241 cggcatgcgc atagtgcggt tcgccggtgg tggggaagtc cagcaacaac tccatcttgt
      301 ccccatcgat gctgaagagc tgtgcgctct gggtgagttc cggtccggtg ggcaggtacc
      361 ggtccttggt gatcttgttg tacgcgatca cgtacttgcc ccagggttgc ttgctgtctc
      421 cgccaggcac gcacaggtgg ccgatggagt agtaggtggg cacgcggtcc accacttcga
      481 gggattttac gttccacttc acgatctcgc tgctcacgaa catggagctg tacgcgaagc
      541 cgttgttgtc gaactcggtg tgcaggggcc cgagaccggg tttcttcacc tcgccgtgca
      601 gcacgctctc atatttcagg acggggatgc cgacgaaatc accatcgaaa tccttgtcgg
      661 cgatcgcctt ttgcatcttg gtgaaggaga acaccggcag cgtggcggcc agtttgccgc
      721 tgcccacgat gaactcgccc gtggggtcca catcgcatcc gtgcgggctc ttcgggcagg
      781 gcatgaagta gagcaggtcg gtgagttctt tgggatccaa ctgcagcacc ttgtccacca
      841 cctggctcgt agccgagtgt acggattcgt cgtaatggtt catgtagtgc ttgccatcga
      901 tcatcttgcc cttgccctcc ttggcgtact gttcagcctt cttccagttc acggccatga
      961 tgaagtcctt gtcgcgctga ctggcgttca cctccagcag gctgtacgcc tgctcggtgt
     1021 tgtaacagct gaagaagaac cagccgtcgc tggggccctt gcccgcgtgc gccagatcga
     1081 aatcaaccgc gggggtcgtg atctggaatg cgatcgacat ggtgctggtg gccgtatccg
     1141 gcttgatgaa actcacggtg cctttgaagt tctccttgta ggtctcgatg ggcacatcgc
     1201 gctgcgcatc ggtacccatg gggatgctga agcgtgtacc tgccaccagg tactcgctgt
     1261 tgggtgtaat gaagggcgaa ctgtggttgc cggaactgtt ggggatctcg atgatgctgg
     1321 ccgtacggaa ggtcttcaga tcgatcatgg ccacccgcgg ggtgttgttg ccgttgatga
     1381 acatgtatct gccgtcggga acgccattgg tcatcgaagc ctccacgtgg tgcgaatcat
     1441 cccagggaat gaacccgtgg gtggtcatga gcatgggctt ggtctcctcg ctgtagcccc
     1501 agccgttctc ggggtcgacg gagaacaccg ggatatcgcg cagcagacgc ccggaaggca
     1561 ggccgtacac ggacatctgc ccgctgaagc caccgctgac gaagttgtag aactcgtcgt
     1621 gtgagccggg tttgatgtac accttggagg ccacatcgcc ggacaccacg gtctgggcgg
     1681 tctgtggtcg acagccctgc atcgtgttga agagcaggaa aaggccgcct gctccactga
     1741 gcaggacgag cgcagaaagg acgggacgtt tcattattca ggtttggtgg ttctgatcag
     1801 agggtgcgca tgaactc
//
LOCUS       DAOQOC010000688         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46220_length_1816_cov_2.723453, whole genome shotgun sequence.
ACCESSION   DAOQOC010000688 DAOQOC010000000
VERSION     DAOQOC010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46220_length_1816_cov_2.723453"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1816
                     /locus_tag="PL106_10800"
     CDS             <1..>1816
                     /locus_tag="PL106_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33597.1"
                     /translation="DPLKTTPGVCGCGVPDTDTDSDGTADCVDGCPNDPNKVAAGQCG
                     CGNPDTDTDGDGTADCNDACPEDPYKIVPGICGCDTPDDDTDSDGLADCIDPCPESPT
                     NDDTDADGTADCFDNCPLDPAKVNPGACGCGNPDLDSDGDTYMDCLDGCPSDPNKIGP
                     GTCGCGVPEIDTDGDGLKDCVDQCPLVVGQPGSPCDDGDPSTENDVLNLSCACTGTPA
                     EQPTTLELTTDNDGSATSWEIVPLGGGPAVCSGSGYPSNSTQTIPCALPDGCWSLRVH
                     DAAMDGMCCMNGIGGYVLRAPNGGRIIDAEDAGIFGATAMENLGFCLPMGNDQLTPSR
                     CDREDYMPSDFIQAVPNPAVQAQYGVGSQTDDGYQFWFFKPNGGYSRRVFITHANSSY
                     IFPSGPDRCSYFRLNSLSTFPLPLNTLLNVRVRSVVNGVYSDFGPACRLKIDTETNCP
                     TTLLVATPGPNMSCGLTGVLLNGSTTLHAVPVSGATRYLFEFSKPGYVRNITSSTSSL
                     VITKWSTWPLEYGNTYNVRVKVSFDNGASYCLFGSSCTMSTASGPQGMAQEPETRGMV
                     EPQASSVLNFWPNPNVTGQLTIELSDVGNGAHTGQVEII"
BASE COUNT          367 a          571 c          549 g          329 t
ORIGIN      
        1 cgatcctttg aagaccactc cgggcgtttg cggctgcggc gttcctgaca ccgataccga
       61 cagcgatggc acagccgact gcgttgacgg ctgtcccaac gacccgaaca aggtagctgc
      121 cggtcaatgc ggttgcggca atccggacac ggacacggac ggcgacggta cggccgattg
      181 caacgatgcc tgcccggagg atccgtacaa gatcgtaccc ggcatctgcg gttgtgacac
      241 cccggatgac gataccgaca gcgacggctt ggcggactgc atcgacccat gcccggaaag
      301 tcccacgaac gatgacaccg acgctgacgg cacggccgat tgcttcgaca attgcccgtt
      361 ggacccggcc aaggtgaacc ctggcgcttg cggttgcggc aaccctgatc tcgattcgga
      421 tggcgatacc tacatggatt gccttgatgg ctgccccagc gatcccaaca agatcgggcc
      481 gggaacctgc ggatgtggtg taccggagat cgataccgac ggagatggcc tgaaggactg
      541 cgtggaccag tgcccgctcg tggttggtca gcccggctca ccctgcgatg atggtgaccc
      601 aagcaccgag aacgacgtgt tgaacttgag ctgcgcttgc accggaaccc cggctgagca
      661 gcctaccacc ctcgagctga ccacggacaa cgatggatcc gcgacctctt gggagatcgt
      721 acccctaggc ggaggaccgg ctgtctgctc gggcagtgga tacccgagca acagtacaca
      781 aacgatcccc tgcgcgcttc cggatggctg ctggtccctg cgggtccacg atgcggcgat
      841 ggacggcatg tgctgcatga atgggatcgg cggatacgtg cttcgtgcgc cgaacggcgg
      901 gcggatcatt gacgctgagg acgcgggcat cttcggtgcc acggccatgg agaacctcgg
      961 attctgcctg cccatgggca acgatcagct cacaccgagc cgttgtgacc gggaggacta
     1021 catgcccagc gacttcatcc aggcggtgcc caacccggct gttcaagcac aatacggcgt
     1081 gggcagccag accgatgatg ggtaccagtt ctggttcttc aagccgaatg gaggctacag
     1141 ccgtagagtg ttcatcaccc atgcgaacag cagctacatc ttccccagcg ggccggatcg
     1201 ttgcagttac ttccgcctga actcgctgtc gaccttcccg ctgcccttga acaccttgct
     1261 caacgttcgc gtgcgcagcg tggtgaacgg cgtgtactcg gacttcggac cggcctgccg
     1321 actgaagatc gacaccgaga cgaattgccc gaccacactg ctcgttgcca ctccgggtcc
     1381 caacatgtcc tgcgggctca ctggggtgtt gctgaacggc tcgaccaccc tgcatgctgt
     1441 accggtatcc ggtgccacgc gctacctctt cgagttcagc aaacccggat acgtgcgaaa
     1501 catcacttcc tccacgtcaa gcctggtcat caccaagtgg agtacctggc cattggagta
     1561 tggcaacacg tacaatgtgc gcgtcaaggt gagcttcgac aatggcgctt cgtactgcct
     1621 cttcggatcg agttgcacca tgagcacggc ttcaggcccg cagggcatgg cccaggagcc
     1681 ggagacccgg ggcatggtgg aaccacaagc gtcgagcgtc ctcaacttct ggccgaaccc
     1741 gaacgtcacc ggacagttga ccattgaatt gagcgatgtg ggtaacggcg cgcacaccgg
     1801 ccaagtggag atcatc
//
LOCUS       DAOQOC010000689         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46331_length_1814_cov_2.689596, whole genome shotgun sequence.
ACCESSION   DAOQOC010000689 DAOQOC010000000
VERSION     DAOQOC010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46331_length_1814_cov_2.689596"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PL106_10805"
     CDS             complement(<1..1019)
                     /locus_tag="PL106_10805"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HNA33598.1"
                     /translation="MAQKIFLSVKVDGTELYAVRSVSISQGIFEHHSFEVVVPGRSID
                     PDPKNATKVMDTIAGWIGKTIVIDWETGSLKAERKGEDQSEFHGIVTNVSVNGQEKDF
                     MTVTISGKSPTILLDGVPNSETYANVGLKDLYNKTIVSAPVNELAAEDKLAFKDKLPF
                     SVQYNETDWNFICRMMHENGEWFYYNGQKIVLGTVKAKTTRITPGHVSSLDFTFGVTR
                     PVGPVRAWDYLKNSEAELKPAEPELSDEYAKKILRSSDDLYPSRKADHVAFPSWPDGD
                     EHHTKKDLLSGRLDKKRQGHANGVFSLVGVCQVGEIQLGSVLSLEGFAHSGEYMVTRV
                     THTCQG"
     gene            complement(1117..>1814)
                     /locus_tag="PL106_10810"
     CDS             complement(1117..>1814)
                     /locus_tag="PL106_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     protein TssC"
                     /protein_id="HNA33599.1"
                     /translation="VMELFQSANLSGGDVYKSNVVMGANWLIGRPKDAAFGEEDHLYV
                     PPAGALAGKIYNTLMSQVTAGKKHGSLNEVDAVAFDLRKSEIAAMEKMGLVPMVNEYG
                     KVMAFSAKTLFNGDNIGLQTYSVVRTFDYISKVLQDFLNRRAFENFNAKTQREIRAQI
                     VKFLDGICGPGKLIEKFKILRFEQDPNQKDRIFLDIHMTPYFPAKNYVFKLDGQKGDD
                     PDSAEWQTEYAQE"
BASE COUNT          291 a          552 c          534 g          437 t
ORIGIN      
        1 ccctggcagg tgtgcgtcac gcgggttacc atgtactcgc ccgagtgcgc gaacccttcg
       61 aggctgagca cgctgcccag ttggatctcg cccacctggc agaccccgac aagggaaaag
      121 accccgttcg cgtgcccctg tcgcttcttg tccagacgcc cgctcagcag gtcctttttg
      181 gtgtggtgct catccccatc cggccacgag gggaaagcca catggtccgc cttgcgcgac
      241 gggtacaggt cgtcagatga tctgagtatt tttttggcgt attcgtcgga gagttccggt
      301 tcggccggtt tcagttcggc ttcgctgttc ttgaggtagt cccaagcgcg cacgggaccc
      361 accggccggg tgaccccgaa ggtgaaatcc aacgaggaaa cgtgcccggg ggtgatccgt
      421 gtggtcttgg ccttcaccgt gcccagcacg atcttctggc cgttgtaata gaaccattcg
      481 ccgttctcgt gcatcatccg gcagatgaag ttccagtccg tttcgttgta ctggacactg
      541 aacggcagct tgtccttgaa ggcgagtttg tcctccgcgg ccagttcgtt caccggtgcg
      601 ctcacgatcg tcttgttgta caggtccttc aggcccacat tggcgtaggt ttccgagttg
      661 ggcacgccgt cgagcaggat ggtggggctc ttgcccgaga tcgtcaccgt catgaagtcc
      721 ttctcctgcc cgttcaccga cacgttcgtc acgatgccat ggaactcgct ctggtcctcg
      781 cctttgcgct cagccttcag gctgccggtt tcccagtcga tcacgatggt cttgccgatc
      841 catcccgcaa tggtgtccat gaccttcgtg gcgttcttcg ggtcgggatc aatggatcgc
      901 ccggggacca ccacctcgaa agagtggtgc tcgaagatgc cttgggatat gctgacgctg
      961 cgcaccgcgt acaactcggt gccgtccacc ttcacgctga gaaagatctt ctgtgccatt
     1021 tgttatgtgg gatatggtat cgttttctgg attcggtagc ggtatgtccc gtgcggtggg
     1081 gggccgccat cgcgcggccc ccgtgttccg cgttgatcac tcttgcgcgt actcggtctg
     1141 ccactcggcg ctgtccgggt cgtcgccttt ctgtccgtcg agtttgaaca cgtagttctt
     1201 cgccgggaag tacggcgtca tatggatgtc caggaagatc cggtccttct ggttggggtc
     1261 ctgctcgaaa cgcaggatct tgaacttctc gatcagcttg ccgggaccgc agatgccgtc
     1321 gaggaacttc acgatctggg cgcggatctc gcgttgtgtc ttggcattga agttctcgaa
     1381 ggcacggcgg ttcaggaagt cctgcagcac tttgctgatg tagtcgaacg tgcgcaccac
     1441 cgaataggtc tgcaaaccga tgttgtcgcc gttgaacagt gtcttggcgc tgaaggccat
     1501 caccttgccg tactcgttca ccatgggtac cagccccatc ttctccatgg ccgctatctc
     1561 gctcttgcgc aggtcgaagg ccacggcatc cacttcgttg agcgaaccgt gcttcttgcc
     1621 cgcggtcacc tgcgacatca aggtgttgta gatcttgccc gccagggcac cagcgggcgg
     1681 aacgtacagg tggtcctcct cgccgaaggc cgcgtccttg ggtcgcccga tgagccagtt
     1741 ggcgcccatc acgacgttgc tcttgtacac atcgcccccg gagaggttgg cgctctggaa
     1801 cagttccatc acat
//
LOCUS       DAOQOC010000690         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46515_length_1810_cov_3.512251, whole genome shotgun sequence.
ACCESSION   DAOQOC010000690 DAOQOC010000000
VERSION     DAOQOC010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46515_length_1810_cov_3.512251"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1810)
                     /locus_tag="PL106_10815"
     CDS             complement(<1..>1810)
                     /locus_tag="PL106_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNA33600.1"
                     /translation="TYKPERDGLFCERIFGPVKDFECHCGKYKRIRYKGIVCDRCGVE
                     VTEKKVRRERMGHIQLTVPVAHIWYFRSLPNKIGYLLGIPTKKLDQIIYYERYVVINA
                     GGAKNKEGVPLQYLDFITEEEYLDILDQVGKDNQYLDDADPNKFIAKMGAEALHDLLK
                     RLDLDSVSYDLRHKASTETSQQRKNEALKRLNVVEAFRDANARRENRPEWMIIKVVPV
                     IPPELRPLVPLDGGRFATSDLNDLYRRVIIRNNRLKRLVEIKAPEVILRNEKRMLQEA
                     VDSLFDNSRKSSAVKSESNRPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPE
                     LKLHECGLPKDMAAELFKPFIIRKLIERGIVKTVKSAKKIVDKKEPVVWDILENVLKG
                     HPVLLNRAPTLHRLGIQAFQPKLIEGKAIQLHPLVTTAFNADFDGDQMAVHVPLGHAA
                     ILEAQLLMLASHNILNPANGAPITVPSQDMVLGLYYITKGRKSEKDKPMKGEGRVFYS
                     PEEIIIAYNEGQLDLHAWIKVRWTDPRSSQSALIETTVGRVLFNEVVPDEVGFVNELL
                     TKKSLRDIIGRVMKECGTAKTAKFLDDIKELGFMSAF"
BASE COUNT          297 a          551 c          553 g          409 t
ORIGIN      
        1 gaaggcgctc atgaagccga gttccttgat atcgtccagg aacttggcgg tcttcgccgt
       61 accgcactcc ttcatcacac ggccgatgat gtcgcgcagg ctcttcttgg tgagcaactc
      121 gttcacgaaa cccacttcgt ccgggaccac ctcgttgaag aggacccggc cgaccgtggt
      181 ctcgatcagt gcgctctggc tgctgcgagg atccgtccag cgcaccttga tccaggcgtg
      241 caggtccagc tgtccctcgt tgtacgcgat gatgatctct tcgggcgagt agaatacccg
      301 gccctcccct ttcatgggct tgtccttctc gctcttccga cccttggtga tgtagtagag
      361 accgagcacc atgtcctggc tgggcaccgt gatcggcgca ccgttggccg ggttcaggat
      421 gttgtggctc gcgagcatca gcaactgggc ctccaggatg gccgcgtggc ccaagggcac
      481 gtgtacggcc atctggtcac catcgaagtc cgcgttgaag gcggtggtca ccagcgggtg
      541 caattggatg gccttgccct cgatgagttt gggctggaac gcctggatac cgaggcggtg
      601 cagcgttggt gcgcggttca ggagcacggg gtgccctttg agcacgttct ccaggatgtc
      661 ccacaccacg ggctccttct tgtccacgat cttcttggcg ctcttcaccg ttttcacgat
      721 cccccgctcg atgagcttgc ggatgatgaa cggtttgaaa agttcggcgg ccatgtcctt
      781 gggaaggccg cactcgtgca gtttcagctc agggcccaca acgatcaccg aacgggcgct
      841 gtaatccaca cgcttaccga gcaggttctg gcggaaccgg ccctgcttgc ccttgagcga
      901 atcgctcagc gacttcaggg gacgattgct ctcgctcttc accgcactgc tcttgcggct
      961 gttgtcgaac aggctgtcca ccgcctcctg cagcatgcgc ttctcgttgc gcaggatcac
     1021 ttcgggcgcc ttgatctcca ccaagcgctt caggcggttg ttccggatga tgacgcggcg
     1081 gtacaggtcg ttcaggtcgc tggtggcgaa acggccgccg tccaagggca ccagggggcg
     1141 cagctcgggc gggatcacag gtaccacctt gatgatcatc cattcggggc ggttctcccg
     1201 tcgcgcattc gcatcacgga aggcctccac cacattgagg cgtttgaggg cctcgttctt
     1261 gcgctgctgg ctggtctcgg tactggcctt gtggcgcagg tcatagctca cgctgtcgag
     1321 gtccaagcgc ttcagcagat catgcagggc ctcggcgccc atcttggcga tgaacttgtt
     1381 ggggtccgca tcgtccaggt actggttgtc cttgcccacc tggtcgagga tatcgagata
     1441 ctcctcttcg gtgatgaaat caaggtactg gaggggaacg ccctccttgt tcttcgcgcc
     1501 tcccgcgttg atcaccacgt agcgctcgta atagatgatc tggtcgagct tcttggtggg
     1561 gatgcccagc aggtagccga tcttgttggg caggctgcgg aagtaccaga tgtgggcaac
     1621 gggcacggtg agttggatgt ggcccatgcg ctcgcggcgc accttcttct cggtcacctc
     1681 cacaccgcag cggtcacaaa cgataccctt gtagcggata cgcttgtact tgccacagtg
     1741 gcactcgaag tccttgacgg gcccgaagat gcgctcgcag aacaggccgt ccctttcggg
     1801 cttgtaggtg
//
LOCUS       DAOQOC010000691         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46522_length_1810_cov_3.003419, whole genome shotgun sequence.
ACCESSION   DAOQOC010000691 DAOQOC010000000
VERSION     DAOQOC010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46522_length_1810_cov_3.003419"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="PL106_10820"
     CDS             complement(<1..1616)
                     /locus_tag="PL106_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33601.1"
                     /translation="MIARTSIALPLFLATLNGIAQHPAAFVENRGQWPKSVTHKADLN
                     GATVWCERGAILIDRFDGPAIRHANDGPDQPDPVVRHHAVRLRFTGANPGPTCEGLGP
                     QHGTYNYFLGNDPGRWARGAHAFSAVLQKDLYPGVDLRVRTEGPSLKYDLILAPEADP
                     SGIRITYEGADGIALMDGRIVIRTSVGDVIEHIPTAYQEIDGEPRTIPCHYTKTGTSI
                     GFLIGAHSADHPVIIDPVLSFSTFSGSTSDNFGYTATYDADGFLYSGSSAFGQGYPTT
                     TGAYQTQHAGGQGLGDGIDVALTKFDTTGQFLIWSTFLGGAGDELPHSMIVNGANELF
                     VYGTTSSLNFPVTNNAYDPSFNGGTATNPTGLGANYVNGSDIFIARLGSNGDALLAST
                     YFGGSQNDGFNGASGLKHNYADEIRGEVLLDANDNVLVASCTASPDLPVTTGAAQMVY
                     GGGPQDGVVLKMDAQLSTLFWSTYFGGSLADAVYSIELDDDDRVFICGGTRSTDLNMP
                     VGAFQPAFQGGITDGFVAVLLPDGSAVEAAT"
BASE COUNT          319 a          527 c          613 g          351 t
ORIGIN      
        1 gtggctgctt caaccgcact gccatccggg aggagcaccg cgacgaagcc atcggtgata
       61 ccgccctgga atgcgggttg gaatgccccg accggcatgt tcaggtccgt actccgggta
      121 ccgccgcaga tgaacacgcg atcatcatcg tccagttcga tcgagtacac ggcatcggcc
      181 agggagccgc cgaagtaggt gctccagaac agggtcgata gttgggcgtc catcttgagg
      241 accacaccgt cctgcggccc tccgccgtaa accatttgtg ccgcacccgt ggtcaccggg
      301 aggtcgggag aggcggtgca actggccacg aggacgttgt cgttggcatc cagcaggacc
      361 tcgccgcgta tctcatcggc gtaattgtgc ttcaatccgc tcgcgccatt gaaaccgtcg
      421 ttctgcgatc ccccgaaata ggtactcgcc agcagggcgt cgccgttcga tcccaaacga
      481 gcgatgaaga tgtcggaccc gttcacgtag ttggcgccca ggccggtggg gttggtggcc
      541 gtgcccccgt tgaagctcgg atcgtacgca ttgttcgtca ccgggaagtt cagtgagctg
      601 gtggtcccgt acacgaagag ttcgttggcc ccgttcacga tcatgctgtg gggcagttca
      661 tcaccggcac cgccgaggaa ggtgctccag atgaggaact ggcccgtggt atcgaacttg
      721 gtgagcgcca catcgatgcc gtcaccaagt ccctgtccac ctgcgtgctg ggtctggtac
      781 gcacccgtgg tggtcggata gccctgcccg aaggccgaac tcccggagta gaggaagccg
      841 tccgcatcgt aagtggccgt gtagccgaag ttgtcgctgg tggacccgct gaaggtgctg
      901 aacgagagca cggggtcgat gatgaccgga tggtcggcac tgtgagctcc gatgaggaaa
      961 ccgatcgacg tacctgtttt cgtatagtgg caggggatgg tgcgcggttc gccatcgatc
     1021 tcctggtagg ccgtgggaat atgttcgatg acatcaccca ccgaggtccg gatgacgatc
     1081 ctgccatcca tgagggcgat gccatccgca ccttcgtagg tgatgcggat gcctgatggg
     1141 tcggcttcgg gcgcgaggat gaggtcgtat ttcaaggatg ggccttccgt gcgcacccgc
     1201 aggtccacac cgggatacag gtctttttgc aacaccgccg agaaggcatg cgcaccgcgc
     1261 gcccaacgtc ccgggtcgtt gccgaggaaa tagttgtacg tgccgtgttg tgggccgaga
     1321 ccctcgcacg tgggaccggg gtttgcaccg gtgaatcgta accgtacggc atggtggcgc
     1381 accacggggt caggctggtc aggaccgtca ttcgcatgcc tgatcgctgg tccgtcgaag
     1441 cggtcgatga ggatggcccc gcgttcgcac cagaccgtgg ctccgttcag gtcggccttg
     1501 tgcgtcacgc tcttcggcca ttgtccacgg ttctcgacga atgccgcggg atgctgcgcg
     1561 atgccgttga gggtcgcgag gaaaagcggg agggcgatgg aggtgcgggc gatcatccca
     1621 gggggagacg ccggaaaact aaggcgcgat gcctgttcag ggcctggtgt acgcctgctc
     1681 gtcgcgcatc accaggcgga agtccacgcg ccggttgagc gcgcggccac gtgatgtggt
     1741 gttcgttgcg atgggagcgt tctcgccgtg gccgatgggg acgagatggt cgctcgcggc
     1801 accaccttga
//
LOCUS       DAOQOC010000692         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46592_length_1809_cov_2.736032, whole genome shotgun sequence.
ACCESSION   DAOQOC010000692 DAOQOC010000000
VERSION     DAOQOC010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46592_length_1809_cov_2.736032"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1809
                     /locus_tag="PL106_10825"
     CDS             <1..>1809
                     /locus_tag="PL106_10825"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33602.1"
                     /translation="LISGLTDEFYTFNSGLNTSVQWELTLGTLQTYGVQVLDGCAGTE
                     IYCSGDALSGNLAVLPNTDYIFRLLGVNQPQFQGTFSLCISTPPAPGCAQAPQPADGG
                     LVCEGSNVELSWGAPPFAGEYDVYLDNVLVATVNSTSYDAGVVSNGAHTWYVVPKNLS
                     GSASGCTVWNFSTGTGSCNCANFCIPTDQTGYGSLITHVVLEDIENTTPDGTLDPAAI
                     TYSLQAATTSLTRNITYPLQVDVDHALDGNGWGGGFTGAWFDWDASGTFDTDEFYPIS
                     TIGYTGTVNVTVPADAALGSIRMRIRSNATYSGGPSFGAGGACGVVLGQNFGETEDYC
                     VTILPLLVSTDPCAPIAILCNDLVWGSTLGEANNIVAGACPFNGAASTGGVNYYSYTA
                     LANEDVTFSTCGTSNFDTRISVFSGSCGDFTCMYGNDDSPGCPGNTSTITLRAVSGET
                     YLIMVSGAGAAEGLYQIGSFCEPYCAPTEANDRCSNAASPTVYLDDDPMALPSTETLG
                     CSYQDLPNACSGSNTVQGVWYTFSTGPDVTSYDLYLASNAENPAYTTPTVDFALYSGT
                     CGSLTEVQCVTNAGGTTALTLAPSTSYRLLVYNQGG"
BASE COUNT          341 a          610 c          544 g          314 t
ORIGIN      
        1 gcttgatcag cggtctgacg gacgagttct acaccttcaa ctcgggcctg aacaccagcg
       61 tgcagtggga actcaccttg ggcacgctgc agacttatgg cgtccaagtg ctggacggct
      121 gtgccggaac ggagatctac tgcagcggcg acgccctctc gggcaacctc gccgtactgc
      181 ccaacacgga ctatatcttc cgtctgttgg gtgttaacca gccccagttc caaggcacct
      241 tcagcctgtg catcagcaca cctccggctc cgggctgcgc ccaagcaccg caacccgccg
      301 acggtggact cgtgtgcgaa ggttcgaacg tggaactgag ttggggcgca ccgcccttcg
      361 ccggtgagta cgacgtgtac ctggacaacg tcctggtagc cacggtgaac agcaccagct
      421 acgacgcggg cgtggtgagc aatggagcac atacctggta cgtggtaccg aagaacctct
      481 cgggatcggc ctcgggctgc accgtgtgga acttcagcac gggcacggga agttgcaact
      541 gcgcgaactt ctgcatcccg acggaccaga cgggttacgg ctcgttgatc acgcacgtgg
      601 tgctggagga cattgagaac accacgcctg acggcaccct cgatccggcc gccatcactt
      661 actcgctgca agcggccacc acgagcctca cgcgcaacat cacctatccg ctccaagtgg
      721 atgtggacca tgcgctggac ggcaacggct ggggcggcgg cttcacgggc gcctggttcg
      781 actgggatgc cagcggcacg ttcgacaccg atgaattcta cccgatctcc acgatcggat
      841 acacgggcac ggtcaacgtg acggtaccgg ccgatgcggc cttgggcagc atccgcatgc
      901 gcatccgcag caacgccacg tactccggcg gtcccagctt cggtgctggt ggtgcctgcg
      961 gcgtggtgct cgggcagaac ttcggtgaga cggaggacta ctgcgtcacc atcctgccgc
     1021 tgctcgtgag cacggatccc tgcgctccga tcgccattct ctgcaacgac ctggtctggg
     1081 gttccaccct gggcgaggcc aacaacatcg tcgccggagc gtgtcccttc aacggcgctg
     1141 ccagcaccgg aggtgtgaac tactactcat acacggccct ggccaacgag gatgtgacct
     1201 tcagcacctg cggtacgtcc aacttcgaca cccgcatctc ggtgttcagc ggctcgtgcg
     1261 gcgacttcac ctgcatgtac gggaacgacg attcgccggg ctgcccgggc aacaccagca
     1321 cgatcacgct gcgtgccgta tcgggtgaaa cctacctgat catggtgagc ggtgccggtg
     1381 cggccgaagg cctgtaccag atcggcagct tctgcgagcc gtactgcgcg cccaccgagg
     1441 ccaacgaccg ctgcagcaac gctgcgagcc ccacggtcta tctggacgat gatccgatgg
     1501 cgctgccctc caccgaaacg ctcggctgct cctaccagga tctgcccaac gcctgctcgg
     1561 gcagcaacac ggtacaaggg gtatggtaca ccttcagcac gggtcccgat gtgacctcgt
     1621 acgacctgta cctggccagc aacgccgaga atccggcata caccacgccc acggtcgact
     1681 tcgcgctgta ttccggtacc tgcggaagct tgaccgaggt gcagtgcgtg accaacgcgg
     1741 gtggcaccac ggcactcacg ctcgccccca gcacctcgta ccgcctgttg gtgtacaacc
     1801 agggcggcc
//
LOCUS       DAOQOC010000693         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46660_length_1808_cov_2.903594, whole genome shotgun sequence.
ACCESSION   DAOQOC010000693 DAOQOC010000000
VERSION     DAOQOC010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46660_length_1808_cov_2.903594"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PL106_10830"
     CDS             complement(<1..573)
                     /locus_tag="PL106_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33603.1"
                     /translation="MRFHFTREPGATVIGTVERGCQEHLSFPLGPSERLELLGDPVIR
                     DPARFPAVALGSDGRVDGSRLFDTAPGHYYWFHWKDDTLECGSSFCGLLPMFFSERNG
                     RLDIASLATGIAASHGRIADDRAWLLERTLFNYPLFDRTPWKGIRRLPAHSKLVVTPE
                     VVKEERFFRVEDHFGDGAGRAAVDMDHLCDM"
     gene            complement(573..>1808)
                     /locus_tag="PL106_10835"
     CDS             complement(573..>1808)
                     /locus_tag="PL106_10835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA33604.1"
                     /translation="SHFRPNLRRGPPWTTTWPPLPNCMPVHSPLVTYYHSVAPELPGA
                     KHWPLSFLTMRMDRFEEELDHIVERGWRTIHLDEWWRMRCGQERSRGNELAITFDDGL
                     LDNWVFAFPLLKERGLKATFFICPELIEPGDHLRPNLEDVWNGRTRMEDLAGRGQLSW
                     GECRALQRSGVVDIQNHTMTHTKHIVSDRLRGVNRGGFEGWYPAMNTCAPDERAYMAK
                     CPDIATYIPIGMPLFEEHSAVVARRKTIRPEFMEAARKLIGEHDLSTPDGRSTHEVRL
                     HALHAAFQARNAVVEGEESFDEHVARVEDEIIGSKRRIEKELDKPVRFLCWPHGGNSP
                     AVHAVARQAGHVATTVGRWVASANERDRIPRTGTGWPLGTTMRRLKFDAKMGAAHGRQ
                     PYRALAGLNAFRHRLFNQG"
BASE COUNT          347 a          576 c          559 g          326 t
ORIGIN      
        1 catgtcgcac aagtgatcca tgtcaacggc cgcgcgaccg gcgccatcgc cgaaatggtc
       61 ctccacacgg aagaatcgct cctccttcac cacctctggc gttaccacga gcttgctgtg
      121 cgcgggaagc cgccggatac ccttccatgg tgtgcggtcg aagagcggat agttgaacaa
      181 ggtccgttcg agcaaccatg cgcgatcgtc cgcgatgcgt ccatggctgg ccgcgatacc
      241 cgtggccaag gatgcgatat ccagccgccc attgcgctcg ctgaagaaca tgggcaggag
      301 tccgcagaat gacgatccac attccaaggt atcatccttc cagtggaacc agtagtagtg
      361 gccaggggcg gtatcgaaaa ggcggctgcc gtccacccgg ccatcggagc cgagcgcgac
      421 agcggggaac cgggccggat cacggatcac cgggtcaccc agcaattcca gccgttcgct
      481 gggacccagc gggaatgaga ggtgctcctg gcatccccgt tccacggtac cgatcacggt
      541 cgcgccgggc tcacgggtga aatggaagcg catcaaccct ggttgaaaag gcggtgcctg
      601 aacgcgttga gaccggccaa cgcgcggtac ggttggcgtc cgtgcgcagc gcccatcttg
      661 gcatcgaatt tcaggcggcg catggtggtt ccgagcggcc atcccgtccc ggtgcgggga
      721 atacgatccc gctcgttggc cgacgcgacc caccggccca ccgtggtggc cacatggccc
      781 gcctgcctgg ccacggcgtg aacagccggg ctgttgccac cgtgcggcca gcagaggaag
      841 cgcacaggct tgtccagttc cttctcgatc ctgcgtttcg acccgatgat ctcgtcctcc
      901 acgcgggcca cgtgctcatc gaacgactcc tcgccttcga ccacggcgtt cctggcctgg
      961 aatgcggcat gcagcgcatg gagccggacc tcatgggtcg aacgaccatc gggcgtggac
     1021 aggtcatgtt caccgatcaa cttccgggcc gcctccatga attcgggacg gatggtcttc
     1081 cggcgtgcga ccacggcaga atgctcctcg aacagcggca tacctatcgg aatgtatgtt
     1141 gcgatatccg ggcactttgc catgtacgcg cgttcgtccg gagcacaggt gttcatggcc
     1201 gggtaccagc cctcgaagcc accccggttc accccacgaa gccgatccga cacgatatgc
     1261 ttggtatgcg tcatcgtgtg gttctggatg tccaccacac cggatcgctg caacgcccgg
     1321 cattcacccc aagagagttg gccacggccc gcgaggtcct ccatgcgtgt acggccgttc
     1381 cacacgtcct caaggttggg ccgcaaatgg tcaccgggct cgatcagttc cggacagatg
     1441 aagaaggtgg ctttgaggcc gcgttccttc aggagcggga aggcgaagac ccaattgtcc
     1501 agcaggccat catcgaaagt gatcgccaat tcgttgccgc gtgagcgttc ctgtccgcag
     1561 cgcatgcgcc accattcatc caggtggatc gtacgccaac cgcgctcaac aatgtggtca
     1621 agttcctcct cgaaacgatc catccgcatg gtgaggaaac tcaaaggcca gtgctttgcg
     1681 cccgggagct cgggggccac cgagtggtag tacgtaacga gcgggctatg cacgggcatg
     1741 cagttcggca atggcggcca ggtggttgtc catggcggac ctctgcgcaa gttggggcga
     1801 aagtgcga
//
LOCUS       DAOQOC010000694         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46893_length_1804_cov_2.361349, whole genome shotgun sequence.
ACCESSION   DAOQOC010000694 DAOQOC010000000
VERSION     DAOQOC010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46893_length_1804_cov_2.361349"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PL106_10840"
     CDS             complement(<1..1455)
                     /locus_tag="PL106_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNA33605.1"
                     /translation="MIRPSLTRTAACLALAATAALSTAQVATFYTTSTDTRTWSPITG
                     GQLIAATTFDDVVYQVMVPSVFFNGTYYTELRVSTNGFITLGGPVPGATNYIPISGSG
                     GYAGAIAPFATNLKNATSGTPEVRWQLVNDEVVVQWLDVARATGGTERFSFQARMNRV
                     TGVISFRYSAVTSLNADATNQPQVGLRGPNNTFATNVKNLMVGTGAETWAAPLSGTAN
                     SSTMRFTATAPAKSPAGNEAFTFTPNCVSPVANTTAVSNCATNNFMVTVHVTSLGTAS
                     GVNIVASPGGTLHSNVGVGDHLCGPFTLGTPVTFSVVNTLNNFCTNVLPVVSPTPTCI
                     THANGTCYDDAPQPVIPDDGCSNGSNLQATIPISGVGTTLGPMPGNAFFQSVELIIAH
                     TFRGDLEVYLTSPTGQKRTMLLHAPTADAEGSNFGNPGSCPSQVLQLRDTGGQPQSSM
                     SPTAPNLSGIYTPVQSLGGFTGNPNGNWVLTICDG"
     gene            complement(1619..>1804)
                     /locus_tag="PL106_10845"
     CDS             complement(1619..>1804)
                     /locus_tag="PL106_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33606.1"
                     /translation="VNTVGVDVMDAVGRVVTTRTLPVQDGVVRSTMSFDETLAPGLYV
                     VNLQAGDRRWTERLVIQ"
BASE COUNT          332 a          524 c          591 g          357 t
ORIGIN      
        1 tccatcgcag atggtgagca cccagttgcc gttggggttg cccgtgaaac cgcccagtga
       61 ttgcaccggg gtgtagatgc ccgagagatt cggtgcggtg gggctcatcg aactctgggg
      121 ctggccaccg gtgtcacgca gttgaaggac ctggctggga cagcttcccg ggttgccgaa
      181 gttgctgccc tcggcgtcgg cggtgggtgc atgcaacagc atggtccgct tctgaccggt
      241 gggagaggtc agatacacct cgaggtcgcc acggaacgtg tgcgcgatga tcagctcgac
      301 ggattggaag aacgcgttgc cgggcatggg ccccagggtg gtacccacgc cactgatggg
      361 gatggtggcc tgcaggttgc tcccgttgga gcagccgtca tccgggatca cgggttgggg
      421 agcgtcatcg taacaagtgc cgttggcatg cgtgatgcag gtgggggtcg gggagacgac
      481 cggcagcacg ttcgtgcaaa agttgttgag cgtgttgacc acgctgaagg tcaccggcgt
      541 tcccaaggtg aagggcccgc acagatgatc gcccaccccg acattgctgt gaagcgttcc
      601 tccgggactg gccacgatgt tcacgccgga ggccgtgccc aagctggtca catggaccgt
      661 caccatgaaa ttgttcgtcg cacaattcga gaccgcggtg gtgttggcca cgggcgatac
      721 gcagttcggg gtgaaagtga acgcttcgtt acccgccggg cttttcgccg gagccgtggc
      781 ggtgaaacgc atggtgctgg aattcgccgt tccgctcagc ggggctgccc aggtctcggc
      841 accggtgccc accatcaggt tcttcacatt cgtggcgaag gtgttgttcg ggccacgaag
      901 acccacctgg ggctggttcg tggcgtccgc gttcaacgag gtcaccgcac tgtaccggaa
      961 actgatgacg ccggtgaccc ggttcatcct ggcttgaaag ctgaaccgtt cggtaccacc
     1021 cgtcgcgcgt gccacatcca accattgcac caccacctca tcgttcacca gctgccagcg
     1081 tacctccggt gtaccgctgg tggcgttctt caggttggtc gcgaatgggg cgatcgcccc
     1141 ggcgtaaccg ccacttccac tgatggggat gtaattggtg gcgcccggta caggaccgcc
     1201 aagcgtgatg aatccgttgg ttgatacccg taattccgta tagtacgtcc cgttgaagaa
     1261 taccgatggt accatcacct ggtacaccac atcgtcgaag gtggtggcgg cgatgagttg
     1321 gccaccggta atggggctcc acgtgcgcgt atcagtgctt gtggtgtaga acgtggcaac
     1381 ttgtgcagtg gacaacgctg ccgttgcggc cagtgccagg catgcggccg ttcgggtaag
     1441 ggatggacgg atcatgggaa ggtgctatcg tgcgcaagtt agggaactca cctgcgccag
     1501 cgaacatatc gccgcgtgtt ggcccggaag ggcacgtggt gatgaagtcc gataaaaata
     1561 aaaggacccc ccgggcatgc ccggggggtc cttgtctcaa gttcctgtga accgtccctt
     1621 attggatcac caaacgctcg gtccagcggc gatcgccggc ctgcaggttc accacataca
     1681 ggcccggggc cagggtctcg tcgaagctca tcgtgctgcg caccacacca tcctgtacgg
     1741 gcagggtacg ggtggtcacc acacggccaa cagcgtccat cacatccacc cctacggtgt
     1801 tcac
//
LOCUS       DAOQOC010000695         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46919_length_1803_cov_3.413043, whole genome shotgun sequence.
ACCESSION   DAOQOC010000695 DAOQOC010000000
VERSION     DAOQOC010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46919_length_1803_cov_3.413043"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..>1803
                     /locus_tag="PL106_10850"
     CDS             289..>1803
                     /locus_tag="PL106_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33607.1"
                     /translation="MYGTQAPFPVTALPQHPLTEENTYHGEIATRSQFVHTDGDCQLV
                     MFGIDGNIYDKDGYLIADVDDGDGNEEDCAQCVEPGVMEMLAIPVPDHCGLYYILSAG
                     HQYGWDSQADDYVVQGVTAVHAAVLDLTRENPYDTDRLGRLVTRDEILDMDYPELYDW
                     METLMNTSMYVASPNGPPDILLQAPQACKSPLVLLRAVRSTALGEDGYWLYASHAGKV
                     VEYRVNSSGIHFVAAIPTYLDPLPIGMTARNYSSDATAIQVDNEVRLALTNGGQPLYA
                     YPDIAGTSPGFGAVVMRFNAQTGALIGSVQGLAPGQDGYPDLVDVNYVAPQAPGPGQD
                     GAAACAWLDNGAKLLVTGREVEGNDWVPIMAVYDMATATWDDILPELGLQGGGADDYV
                     RSRAHRNTAPDGIGDAIYLPHPAGIAAITGVDNGNYAFTAAVPTVAPCIPPVLDLLAN
                     VLYEPRFINAQVAGDNSVEQYNRAECCTFRTTYEGYNGHTFPNIPGTYHWYPDDN"
BASE COUNT          378 a          518 c          541 g          366 t
ORIGIN      
        1 ccgcttgcgt gatccatctg ctttgtaccg cacccatcgc cgttcctcgc gccagcacgg
       61 ccatacctca tttcgggcat ggtggttccg gcaggttctc ctgctttggg tggtgttact
      121 cgacgtactg agccaacaac aacagaccga tgaaaacaca tttcaggctg aaggccgcgc
      181 tgtgcgtggc gctgtgtatc cctctatggg ggggggtatt ttgaatcgct cacgccgcag
      241 gccagggggc aggcggaggc ttgggcgatc gggaatcaac gggtggacat gtacgggacg
      301 caagcgccct tcccggtgac cgccttgccg caacacccat tgaccgagga gaacacctac
      361 cacggcgaga tcgccacacg atcccaattc gtacacaccg atggcgattg ccaattggtg
      421 atgttcggta ttgatgggaa catctacgac aaggatggtt acctgatagc ggatgtggat
      481 gatggtgatg gaaatgagga ggattgcgca cagtgcgtcg aaccgggggt aatggagatg
      541 ctggccattc cagttccgga tcattgcggg ttgtactaca tcttgagtgc cggccatcaa
      601 tacggatggg acagtcaagc ggatgattac gtggttcaag gtgtcactgc ggtgcatgca
      661 gcggtgttgg acctgacccg ggagaacccg tacgatacgg accgactcgg caggcttgtg
      721 acccgtgatg agatccttga catggattac ccggagcttt acgattggat ggagacattg
      781 atgaacacat ccatgtacgt ggcaagtccg aatggtccac cggacatcct gttgcaagct
      841 ccgcaagcat gcaaatcgcc gctggtccta ctccgggctg tgcgtagcac ggccttgggc
      901 gaagatgggt attggctcta tgcctcgcat gcgggcaagg tggtggaata tcgggtcaat
      961 agttcgggta tccattttgt tgccgcaata cccacctatc tggatccact ccccattggc
     1021 atgaccgctc gaaactattc gagcgatgcc accgcgatcc aagtggacaa cgaggtgaga
     1081 ttggcactca ccaacggagg ccaacccctg tacgcgtacc ccgatatcgc tggtacgtcg
     1141 cccggcttcg gtgcggtggt catgcgtttc aatgcgcaaa ccggtgcgct gatcggcagt
     1201 gtgcaaggcc tggcacccgg ccaggatgga taccccgatc tggtggatgt gaattatgtg
     1261 gcgccgcaag caccagggcc cgggcaggat ggtgccgccg cctgcgcctg gttggataat
     1321 ggtgccaaat tgttggtcac gggccgggag gtggaaggga acgactgggt ccccatcatg
     1381 gcggtgtacg acatggccac ggccacctgg gacgatatcc tgccggaatt gggacttcaa
     1441 ggtggagggg cggacgacta cgtgcgcagc cgcgcccacc gcaacaccgc accggatggc
     1501 atcggggacg ctatctacct accgcacccc gcaggtatcg cggccatcac gggtgtggat
     1561 aacggcaact atgcattcac tgccgccgtg cccacagttg caccctgcat accacccgtc
     1621 ctggaccttc tggcgaatgt cttgtacgaa ccccgcttca tcaatgccca ggttgctggc
     1681 gataacagcg tagaacaata caaccgtgcc gagtgttgca cgttccgcac cacctacgaa
     1741 ggttacaacg gccacacctt ccccaacatc cccggcacct accattggta ccccgatgac
     1801 aac
//
LOCUS       DAOQOC010000696         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_46925_length_1803_cov_2.978261, whole genome shotgun sequence.
ACCESSION   DAOQOC010000696 DAOQOC010000000
VERSION     DAOQOC010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46925_length_1803_cov_2.978261"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..391
                     /locus_tag="PL106_10855"
     CDS             98..391
                     /locus_tag="PL106_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33608.1"
                     /translation="MKEWNATRYTSALVIIHPGYLSAMAAATKRTGKKKRADDPTGNT
                     VSEPQAVYTRKAPRRNKPKQPSPQGYNAKRSAGMIPGLATRMKEYLKSMRDER"
     gene            381..749
                     /locus_tag="PL106_10860"
     CDS             381..749
                     /locus_tag="PL106_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA33609.1"
                     /translation="MSGDPLLVDTNILIHHWNGDPRTTRLLDNKLIHISFISEIEVLG
                     FHGYTAHERAKVIADLAFIRIIDMDAGIKAVAIDLCAKDRMKLADALIAATAIRTGIP
                     LVTEDKHFRKLKGMLELVEL"
     gene            1161..>1803
                     /locus_tag="PL106_10865"
     CDS             1161..>1803
                     /locus_tag="PL106_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33610.1"
                     /translation="MIRKNRIGPTAGLINLFNGYWAVLARDTEFQRRFFLPFYHLGNE
                     RSGLWKLHTLPGFQHALTRSNSVKSLGALLAFDAYAELRPDVFERWLNHEHRAEDRML
                     LMAAYFTGQPIPVQLPDHLQELERQIETDTPEAYVARQLRRVQEEEEEEQVLRSAAFK
                     RKIPELYGHRCAITGLQVSSDRPASLIDACHIAPWADSFDDTISNGIALCPNMH"
BASE COUNT          375 a          557 c          532 g          339 t
ORIGIN      
        1 ttctgcttga ccccatgatc gcgggagccg tcatggcctt gagcagcgtg agcgtggtga
       61 gcaatagctt aaggttgagg tgggtgaaac ttggcgtttg aaggaatgga acgcgacccg
      121 gtacacttca gcgctggtca tcattcaccc tggttacctt agtgccatgg ctgctgcaac
      181 caaacggacc gggaagaaaa agcgtgcgga tgaccccaca ggcaacacgg tcagcgagcc
      241 acaagccgtg tatacgcgaa aggctccgcg gcgcaacaag ccaaagcagc caagcccaca
      301 gggctacaac gcgaaacgct cggccggcat gatcccgggc ttggctaccc gcatgaagga
      361 atacctgaag tcgatgcgcg atgagcggtg atcccttgtt ggtggatacc aacatcctga
      421 tccaccactg gaacggtgac ccgcgcacca cgcgcttgct ggacaacaag ctgatccaca
      481 tctccttcat ctcggagatc gaggtcctgg gcttccatgg atacacagcc cacgaacgtg
      541 ccaaggtcat cgcggacctg gcgttcatcc gaatcatcga catggacgcg ggcatcaagg
      601 ccgtggcgat cgacctgtgc gccaaggacc gcatgaagct cgccgatgcc ttgatcgccg
      661 ccacggcaat acgcaccggg atcccgttgg tcaccgagga caagcacttc agaaagttga
      721 aggggatgct ggaattggtg gaactctgaa ctgaagtggg cgaggctggc gtgagggagt
      781 ggcttcctga gcaatgttga aggatgggcg tgcccccttg ccagcgcaca acgccacgct
      841 tggggtcggg ccatccgctg tagccccgtc cgtccgcgcc gcgcgggtgg cgctgcgggg
      901 tcacctcgtg cctcggtgcc tcctcgctgc acacccgcgc aaggcgctgc cggccggggg
      961 ctgccgctgc tgtccctcac gcaaagctgc cccactgctt gcgcttcagc ctctgatgcg
     1021 cacctttcgg gtgcagttcc caaatactcg gcatgccagt gtttcccctt cccgaccatt
     1081 tacggctcaa acgtggagga ggcaatgccc cccacaagcc tatcctgctg ctggctgtgc
     1141 tccgggcctt cgatgatggt ttgatccgca agaatcgtat cgggccgacg gcagggctca
     1201 tcaacctgtt caacggttat tgggcggtat tggcacggga cacggaattc cagcgccgct
     1261 tctttctccc gttctaccat ttgggcaatg agcgctcagg actttggaaa ctgcataccc
     1321 tgccaggatt ccagcatgcg ttgacaaggt cgaactcggt gaagagcctt ggtgcgctcc
     1381 tcgcgttcga tgcatacgct gaactgcgcc cggatgtctt tgagcgatgg ttgaaccatg
     1441 aacaccgggc agaagaccgg atgcttctga tggctgcata cttcacggga caacccattc
     1501 ccgtgcaact gcccgaccac cttcaggaac tcgagcgaca gatcgaaacg gacacccccg
     1561 aagcctatgt cgcccgacaa ctgcggcgtg ttcaggagga agaggaggaa gagcaggtgc
     1621 tgcgcagcgc cgccttcaag cgcaagatcc ccgagttgta cggtcatcgc tgtgcgatca
     1681 cgggattgca ggtaagcagc gaccggccgg ccagcctgat cgatgcctgc cacatcgcac
     1741 cctgggccga ttcattcgac gataccatca gcaacggcat cgccttatgc ccgaacatgc
     1801 acc
//
LOCUS       DAOQOC010000697         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47032_length_1801_cov_3.079611, whole genome shotgun sequence.
ACCESSION   DAOQOC010000697 DAOQOC010000000
VERSION     DAOQOC010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47032_length_1801_cov_3.079611"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PL106_10870"
     CDS             complement(<1..348)
                     /locus_tag="PL106_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA33611.1"
                     /translation="MNISSISINRPVLASVISILIVLFGIVGFTYLGVREFPSVDPPV
                     ITVTTNYQGANADIMESQVTEILEESINGIAGIRSLTSISSDGRSSITVEFNLEADLE
                     AAANDVRDRVSRAV"
     gene            complement(472..1557)
                     /locus_tag="PL106_10875"
     CDS             complement(472..1557)
                     /locus_tag="PL106_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA33612.1"
                     /translation="MTRSMRIIYILPLLLAGCGTGGNDQAQGRGPGGPMQVLVKVLRP
                     EPLSNKVQATGSLLANESVEVRSEVAGRIGSIGFQEGSTVGKGQELVRINDDDLQAQL
                     RKAGLAVQLAQDAEARKKQLLAVQGISQQDYDDARIALQSAEADRDNLQALIAKTVIR
                     APFSGKIGLRQVSQGGYVSPNTLITGLQQVSPIKVDFTVPERFGRDLKAGQRIAFTLE
                     GDTTRHTGEIYAIDPAVDVDSRTIGVRALNANNDGSLRPGSFVRVNLELAHVPDALMI
                     PTEALIPDIQGQKVLLVKGGKAVSARVETGIRTNNEVQLTAGVQAGDTVITTGLLQLR
                     DGMPVKVAPPEKADAMSGSDSTMIDAE"
     gene            complement(1554..>1801)
                     /locus_tag="PL106_10880"
     CDS             complement(1554..>1801)
                     /locus_tag="PL106_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33613.1"
                     /translation="KGYVERCDCIRDRRKYLVKPTPKARPALRAIHKAYAALNDEALA
                     GLKKADRAAFLGQLMMVVSNLHKDAAPVAITNKRVHA"
BASE COUNT          271 a          592 c          568 g          370 t
ORIGIN      
        1 cacggcacgg ctcacgcggt cgcgcacgtc gttggccgcc gcctccaggt ccgcctccag
       61 gttgaactcc acggtgatgc tgctgcggcc atcgctgctg atgctggtga gcgagcggat
      121 gccggcgatg ccgttgatgc tctcctccag gatctcggtg acctggctct ccatgatgtc
      181 cgcgttggcg ccctggtagt tggtggtcac ggtgatcacc ggcgggtcca cgctggggaa
      241 ctcacgcacg ccgaggtagg tgaagcccac gatgccgaac agcacgatga gaatgctgat
      301 caccgaggcc agcacgggcc ggttgatgct gatggaactg atgttcattc agtgaacggt
      361 gatggggaat gggtggtcag ggatcggtga agggtgatca gtgacagggt gccagggtgc
      421 gagagccgtg ccatcgcacc atcgcactct tgcaccctcg catggttccg ctcattcagc
      481 atcgatcatg gtgctgtcgc tcccgctcat cgcatcggcc ttttccgggg gcgcgacttt
      541 caccggcatg ccatcgcgca gttgcagcag accggtggtg atcacggtgt cccccgcctg
      601 cacgccagcg gtcaattgta cttcattatt ggtgcgtatg ccggtctcca cacgtgccga
      661 cacggctttt ccccccttga ccagcagtac cttttgccct tggatgtcgg ggatcagcgc
      721 ctcggtgggg atcatcagcg cgtccggcac gtgcgccagc tccaggttca cgcgaacgaa
      781 ggagccgggc cgcagacttc cgtcgttgtt ggcgttcagg gcccgcacgc cgatggtgcg
      841 cgaatccacg tccacggccg gatcgatcgc gtagatctcg ccggtgtgcc gcgtggtatc
      901 cccctccagc gtgaaggcga tccgctggcc ggccttcagg tcgcgcccga aacgctcggg
      961 cacggtgaag tccaccttga tcgggctcac ctgctgcagg cccgtgatca acgtgttcgg
     1021 cgacacgtag cccccctggc tcacctggcg cagcccgatc ttcccgctga acggtgcgcg
     1081 gatcacggtc ttcgcgatca gtgcctgcag gttgtcgcgg tcggcctcgg cgctctggag
     1141 ggcgatccgt gcatcgtcgt agtcctgctg gctgatgcct tgaacggcca gcaattgctt
     1201 cttccgggcc tcagcatcct gcgccaactg caccgccagc ccggccttgc gcaattgcgc
     1261 ctgcagatcg tcgtcgttga tgcgtaccag ttcctggccc ttgcccacgg tactgccttc
     1321 ctggaagccg atggacccga tgcgtcccgc cacctcgctg cgcacctcca ccgattcgtt
     1381 ggccagcagg ctgcccgtgg cctgcacctt gttggacagg ggctcgggcc gcagtacctt
     1441 caccagcacc tgcatggggc cgccgggacc gcggccctgg gcttggtcgt tgccgccggt
     1501 gccgcacccg gccagcagca ggggaaggat ataaatgatc ctcatcgatc gggtcatgcg
     1561 tgtacgcgtt tgttcgtgat cgccacgggc gcggcgtcct tgtgcagatt gctcaccacc
     1621 atcatcagtt gccccaggaa agcagcgcgg tccgccttct tcagtccagc gagcgcctca
     1681 tcgttcagtg cggcataggc cttgtggatg gctctcaatg ccgggcgtgc cttcggggtc
     1741 ggtttcacga ggtacttgcg acgatcgcgg atgcaatcgc agcgctcaac atagcccttc
     1801 t
//
LOCUS       DAOQOC010000698         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47070_length_1800_cov_3.693983, whole genome shotgun sequence.
ACCESSION   DAOQOC010000698 DAOQOC010000000
VERSION     DAOQOC010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47070_length_1800_cov_3.693983"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PL106_10885"
     CDS             <1..443
                     /locus_tag="PL106_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33614.1"
                     /translation="LLALRAARIQYRDGLIGVDEVMRRMVFNPIYDQINMNSFNYVNA
                     TFDNLLFRYPTQAEFDAGYTMVDGNTPALLFGQSGQNKGDYSGIITSNTEFWEGMVRW
                     CYRTFMGREPSSYETYGVLPGLLADHDLQRVQRAILVTDEYANF"
     gene            446..1000
                     /locus_tag="PL106_10890"
     CDS             446..1000
                     /locus_tag="PL106_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33615.1"
                     /translation="MSMKDPGRTKSIPHSTQHTASGRWQEARGLLLAVSSLLLIASCR
                     KEPIYELNDVGIGPPSANKDQPKTNEQYVSILYANLFQNGLNASEVYGIGQCIESIGD
                     KELAREVIISNFMNKPGVMIPSDSAMRADIDAFVIDTYRRFLVRDPDEAERTWFHNRI
                     EADPNITPEIVYFSFAMSDEYCFY"
     gene            1016..>1800
                     /locus_tag="PL106_10895"
     CDS             1016..>1800
                     /locus_tag="PL106_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNA33616.1"
                     /translation="MDRRKFIKRAGAAAAGTIIAPYILPSGRLFASSGGSGLAQHVVL
                     VMFAGGVRQQESVLQRYLGDSQGEPYEGNILYNLLDGAPPTQKIVYGTGLGGINPIPG
                     ILGQTLGSQGTLFAEMRAVNAGHYGGLNSLLQGTQATTQGLTQKPINPTLFEYLRRHA
                     GVPATKTWFVGNGIGNSIPLLNYSLHPQYGPRYGANFFAPNITFGSLGDQYLGDAKVY
                     HPDDQLAPMYEMKAFLDNSFGHMSEALMDIGNTPEEKEDIKAF"
BASE COUNT          397 a          593 c          499 g          311 t
ORIGIN      
        1 gactcctcgc cctgcgcgcg gcccgcatcc agtaccgcga tggcctgatc ggtgtggacg
       61 aggtgatgcg ccggatggtg ttcaacccga tctatgacca gatcaacatg aacagcttca
      121 actacgtgaa cgccaccttc gacaacctcc tgttccgtta ccccacccag gccgagttcg
      181 acgcgggcta caccatggtg gacggcaaca cccccgccct gctcttcggc cagagcggcc
      241 agaacaaggg cgactactca ggcatcatca ccagcaatac cgaattctgg gaaggcatgg
      301 tgcgctggtg ctaccgcacg ttcatggggc gtgaacccag cagttatgaa acgtacggcg
      361 tcctgcccgg cctcctggcc gatcacgatc tgcagcgggt gcaacgcgcc atactggtca
      421 cggacgaata cgcgaatttc tgaccatgag catgaaagat cccggacgaa ccaagagcat
      481 tccgcacagc acgcagcaca cagcaagcgg caggtggcag gaagcgcgcg gcctgctgct
      541 tgctgtgtcc agcctgctcc tgatcgcctc gtgcagaaaa gaaccgatct acgaattgaa
      601 tgacgtgggt atcgggccgc ccagtgcgaa caaggatcag cccaagacca acgagcagta
      661 cgtgtccatc ctctacgcga acctgttcca gaacgggctg aacgcgagcg aggtgtacgg
      721 tatcggccag tgtatcgaga gcataggcga caaggaactc gcccgcgaag tgatcatcag
      781 caacttcatg aacaaacccg gtgtgatgat cccctccgac tcggccatgc gtgccgatat
      841 cgacgcgttc gtgatcgata cctaccgtcg tttcctggtg cgggaccccg acgaggccga
      901 gcggacctgg ttccacaacc gcatcgaggc cgatcccaac atcaccccgg agatcgtcta
      961 cttctccttc gccatgagcg acgagtactg cttctactga aacatcacca ccaacatgga
     1021 tcggagaaaa ttcatcaaac gcgcaggtgc ggccgctgcg ggcacgatca ttgcccccta
     1081 catcctgccc agtggccgcc tcttcgcgtc gagcggcggt agtggccttg cgcagcacgt
     1141 ggtgctcgtg atgttcgccg gtggtgtgcg ccagcaggaa agcgtgctgc aacgctatct
     1201 cggcgacagc cagggcgaac cctacgaggg caacatcctg tacaacctgc tcgacggcgc
     1261 cccgcccacg caaaagatcg tctacggcac cgggctgggc ggtatcaacc cgatcccggg
     1321 catcctcggt caaacactcg gttcgcaggg tacgctcttc gcggaaatgc gcgccgtgaa
     1381 cgccgggcac tacggtggtc tgaactccct tcttcaaggt acacaggcca ccacccaagg
     1441 cctcacgcaa aaaccgatca accccaccct tttcgagtat ctgcgtcgtc acgccggtgt
     1501 gcccgccacc aagacctggt tcgtgggcaa cggcatcggc aacagcattc ccctgctcaa
     1561 ctacagcctg catccgcagt acgggccccg ttatggcgcc aacttcttcg cacccaacat
     1621 cacgttcggg tcgctgggcg accagtacct gggcgatgcc aaggtgtacc acccggacga
     1681 tcagctcgcg ccgatgtacg agatgaaggc cttcctggac aacagcttcg ggcacatgag
     1741 cgaggcactc atggacatcg gcaacacccc tgaggaaaag gaggacatca aggccttcat
//
LOCUS       DAOQOC010000699         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47208_length_1798_cov_1.952381, whole genome shotgun sequence.
ACCESSION   DAOQOC010000699 DAOQOC010000000
VERSION     DAOQOC010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47208_length_1798_cov_1.952381"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PL106_10900"
     CDS             complement(<1..1027)
                     /locus_tag="PL106_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33617.1"
                     /translation="MALIETAYNPALQDSPVNLYNTTAPGSPYGTTYSATSTLLLQNY
                     IDPSIIRSFPAQFTDIAFLMSLPQAPTPSLEFIWPEMPYLVAPLVAGTGGAGGASPQV
                     YNVTAATAGFVGLNDKVMFPNGQHGVVIAIAGQDITVRSYDAAALPALAAGELLANRG
                     PATADGVSDVMTTFRPQIAQYSNIIEDVGPIAVRFDPKEMKQWERQGTTDYITKTMQA
                     AYERFLVGIHQTAWMSQYGRTTLTNNQTATATAGILQQQANAGVPNQPCTPATFVDVM
                     RQTIFDTALQGSSERVVFATRRVLNIIGTSQKAEKIRYRPEDERWNLDIYEYEFYGHK
                     LILVPMDQ"
     gene            complement(1054..>1798)
                     /locus_tag="PL106_10905"
     CDS             complement(1054..>1798)
                     /locus_tag="PL106_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33618.1"
                     /translation="EALKASLKRVEDSNPALHAAILEELEGRDGLAYISKLPNANILG
                     KKPTDLNDETLLRTYLGDKFTEDEWKAYRTGDYDEIGLTKEIMEAKVKALRPVAEHMH
                     ETRNQEYRSGIQQREEAYRQQREKYNEVLVKSVAKAQEDLFAKLYLNQETIEAFRSGT
                     LSKGLLTDEDGATPHPNMLAALIKAKHFDEAVKRAFEAGKKEGEQRGLKQGVAQLPTP
                     GPGGRVTAPADGQKVDPYAETLAKALNAR"
BASE COUNT          280 a          539 c          587 g          392 t
ORIGIN      
        1 actggtccat cggtacgagg atcagcttgt ggccgtagaa ctcgtactcg tagatgtcga
       61 ggttccagcg ctcgtcctcc ggacggtagc ggatcttctc ggccttctgg ctcgtgccga
      121 tgatgttcag cacgcggcgg gtggcgaaca ccacacgctc gcttgagcct tgcagggccg
      181 tgtcgaagat cgtctgacgc atcacgtcca cgaacgtcgc gggcgtgcag ggctggttgg
      241 gcacaccagc gttggcctgc tgctgcaaga tgcccgcagt ggcggtggcc gtctggttgt
      301 tggtcagggt cgtgcgaccg tactggctca tccaagccgt ctggtggata cccaccagga
      361 accgctcata ggcggcctgc atcgtcttgg tgatgtagtc ggtcgtcccc tgacgctccc
      421 actgcttcat ctccttgggg tcgaagcgca cggcgatcgg acccacatct tcgatgatgt
      481 tactgtactg ggcgatctgc ggacggaagg tggtcatcac gtcgctcacg ccgtcagccg
      541 ttgccggacc gcggttggcg agcagctcac cagcggccag cgccgggagg gcggcggcat
      601 cgtaggagcg caccgtgatg tcctgaccgg cgatggcgat caccacacca tgctggccgt
      661 tggggaacat caccttgtcg ttcaggccca caaagccggc cgtagcagcc gtcacgttgt
      721 agacctgcgg ggaagcacca ccagcgccgc cggtacccgc cacgagaggg gccaccagat
      781 acggcatctc cggccagatg aactccaggc tcggggtcgg ggcttggggc agggacatca
      841 ggaaggcgat gtcggtgaac tgcgccggaa agctccggat gatgctgggg tcgatgtagt
      901 tctggagcag cagggtgctg gtcgcagaat acgtcgtacc gtagggacta ccgggggcgg
      961 tagtgttgta gaggttgacc gggctgtctt gtagcgccgg gttgtatgcg gtctcgatga
     1021 gagccatcgt tcgtttctgt taggggtgcg tgctcatctc gcgttaagag ccttggccag
     1081 tgtttccgcg tacggatcaa ctttctgccc gtcggcagga gccgttacgc gaccacccgg
     1141 accgggggtg gggagctggg caactccctg cttcagaccg cgctgctcac cctccttctt
     1201 gccggcctcg aacgctcgct tcacagcttc gtcgaagtgc ttggccttga tcagggccgc
     1261 aagcatgttg ggatgagggg ttgcgccgtc ctcgtcggtg aggaggccct tggaaagcgt
     1321 gccgctccgg aacgcttcga tcgtctcttg gttcaagtac aacttggcga agagatcctc
     1381 ttgggccttt gccacgctct tgacgagcac ctcgttgtac ttctcgcgct gctggcgata
     1441 tgcctcttcg cgctgctgga taccgctacg atactcctga ttgcgggtct cgtgcatgtg
     1501 ctcggccacc gggcgcaagg ctttcacctt cgcctccatg atctccttgg tcaggccgat
     1561 ctcgtcgtag tcgcccgtgc ggtacgcctt ccactcgtcc tcggtgaact tgtcgccgag
     1621 gtaggtgcgc agcaaggtct cgtcgttcag gtccgtgggc ttcttcccaa ggatgttggc
     1681 gttgggcagc ttggagatgt aggccagccc atcgcgacct tccagttcct ccagaatggc
     1741 ggcgtgcaga gcagggttgc tgtcctccac gcgcttcagg gatgccttca gtgcctca
//
LOCUS       DAOQOC010000700         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47274_length_1796_cov_4.240666, whole genome shotgun sequence.
ACCESSION   DAOQOC010000700 DAOQOC010000000
VERSION     DAOQOC010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47274_length_1796_cov_4.240666"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PL106_10910"
     CDS             <1..230
                     /locus_tag="PL106_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33619.1"
                     /translation="KDTSGPSMNILIKLTSIVALIIAPHINEGDHAAPAEVPTSTSST
                     DRSQNSSDGSVAPGKQEAATDVKTLDLSDRF"
     gene            397..1125
                     /locus_tag="PL106_10915"
     CDS             397..1125
                     /locus_tag="PL106_10915"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNA33620.1"
                     /translation="MAILLFFVAHWYLSLFVQTFYLHRYSAHRMFTMSPFWERFFHVL
                     TWLFQGSSYLNPAIYGMMHRVHHVFADTPEDPHSPKYDGTLFRMMWKTAGWYNGLVTG
                     DKRLDERFTRDLPRWPFLERLAESWTSRIGWMLLYTAFYAVFATAWWQWLLLPLQCVM
                     GPLHGAIINWYAHKYGYVSFPTNDTSKNMWPVEVLMMGEGLHNNHHTHASSANFAKKW
                     WEFDPSWPVIRVLHAIGVIKLRTV"
     gene            1253..>1796
                     /locus_tag="PL106_10920"
     CDS             1253..>1796
                     /locus_tag="PL106_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNA33621.1"
                     /translation="MGDPYAIRYPNDRYFLTFTVVEWLDIFTRLSHRQVLVESLNFCV
                     KNKGLIINAWVIMSNHVHLVARAEEDKHLGHLIRDFKSYTSKAVRDSIIAGPESRQEW
                     LLDKMAFMARKTQRTENFKLWQDGSHAMDLMSDEFLRQKINYIHNNPVRNGLVDLPEH
                     YVFSSARDYFTDQKGLVEIEL"
BASE COUNT          362 a          562 c          499 g          373 t
ORIGIN      
        1 tcaaggacac cagcggtccg tcgatgaaca tcctcatcaa gctcacctcc atcgtggcgt
       61 tgatcatcgc accgcacatc aacgaaggcg accacgcagc gccggcggaa gttcccacga
      121 gcaccagcag caccgaccgc tcacagaaca gcagtgatgg gagcgttgct cctggtaaac
      181 aggaggccgc cacggatgtg aagacactcg atctgagcga ccgtttctga cctttggaat
      241 atccaaggag aggtgcgccg ttggtgcgcc tcttctcttt ttcggcagat gacgtcgatg
      301 gcacgatcgc tatcctgttc gcacgtgatc ctgcggtcct ctgcctgatc cgcgtcatca
      361 gcgttccatc agccccggtc taatttcgcc gccaacatgg ccatcctgct ctttttcgtt
      421 gcgcactggt acctgtcgct cttcgtgcag accttctatc tgcatcgcta cagcgcgcac
      481 cggatgttca ccatgagccc gttctgggag aggttcttcc acgtcctcac ctggctcttc
      541 cagggtagca gctacctgaa tccggcgatc tatggtatga tgcaccgggt gcaccacgtc
      601 tttgccgaca ctcccgagga cccgcactca ccgaaatacg atggtaccct cttccgcatg
      661 atgtggaaga cggcaggctg gtacaacggc ctggtcaccg gtgacaagcg gctcgatgaa
      721 cgcttcacgc gtgacctgcc gcgctggccc ttcctggagc ggctggccga aagctggacc
      781 tcgcgcatcg gctggatgct gctgtacacg gccttctacg cggtcttcgc caccgcatgg
      841 tggcaatggc ttttactccc gctccagtgt gtgatgggac cgctgcacgg cgccatcatc
      901 aactggtacg cgcacaagta cggctacgtg agctttccca ccaatgatac cagcaagaat
      961 atgtggccgg tggaagtgct catgatgggc gaaggcctgc acaacaacca ccacacccat
     1021 gcctccagcg ccaacttcgc gaaaaagtgg tgggaattcg atccctcctg gccggtgatc
     1081 cgagtactgc acgcgatagg ggtcatcaag cttcgtacgg tatgaagggc gctgcatgcg
     1141 cctacctcac ctggtccgcg ccggaggcgc ggatcggcat cgcgtccgcc cgcgcttgta
     1201 gcgcgggcgt gtgaggttcg cgccttgggc gcacttttct catcttccca gcatgggtga
     1261 tccctacgcc atccggtatc ccaacgaccg gtacttcctc acgttcacgg tggttgaatg
     1321 gcttgacatt ttcacgcgct tgtcgcatcg gcaggtcctg gtggaaagcc tgaacttctg
     1381 tgtgaagaac aagggactga tcatcaacgc gtgggtgatc atgagcaacc acgtccattt
     1441 ggtggcccgc gccgaagaag acaagcacct gggccacctc atccgtgact tcaagtccta
     1501 tacctccaag gcggtgcggg acagcattat cgcaggcccg gagagccgtc aggagtggtt
     1561 gctcgataaa atggccttca tggcccgcaa gacacagcgc accgagaact tcaagctctg
     1621 gcaggatggt agccacgcca tggacctcat gtcggacgaa tttctgcgcc agaagatcaa
     1681 ctacatccac aacaaccctg tccgcaacgg tctcgtggac ctgccggaac attatgtgtt
     1741 cagctcggcg cgggactatt tcacggacca gaagggcctg gtggagatcg agctac
//
LOCUS       DAOQOC010000701         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47361_length_1795_cov_2.761494, whole genome shotgun sequence.
ACCESSION   DAOQOC010000701 DAOQOC010000000
VERSION     DAOQOC010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47361_length_1795_cov_2.761494"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PL106_10925"
     CDS             <1..1531
                     /locus_tag="PL106_10925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNA33622.1"
                     /translation="HIEQDLREEAAFVAAHDSLSDAMLRIRWTPMLQSNWVLTAVALA
                     NEAGEEQWVERIGDNWRSGSTIDAQANAQPVIMEWPMTGSPDSARIHIGEPGPDPRRA
                     LWFGQALGEHTSGATWSTGSGPDGSMVLFASMIIRSNQEDRPYRVIRFTISPEVLVRS
                     ITESSQLYGMFHLDPEGRPWKLPDDQRSAVPISRAVDQWSKTLTESPFSFQLGDQRYM
                     AAVQPNTINGIRLYTGGVVEMDPIRARSRGERIVLWSASVLLVVLGGLLAGAYRRGRQ
                     REQRAERRERQSITQERRLAKVISERETLDREVHHRVKNNLQVVSSLLHLQAERVADP
                     GTRNEFTRGKRRIDSMALVHHKLYAQTDLRAIDLRLFMDQIANAVRAMHEPTSRGVSH
                     AVDTGGITANADTAIQLGMILCELLSNAFLHAFPYITGGHIDIEVREAEDGMYRMTVR
                     DNGKGIERQGQDNGAELGLELAEGLAEQIDGRLEITVNAGTRVDVYFRMQGEAPVHGV
                     K"
     gene            complement(1533..>1795)
                     /locus_tag="PL106_10930"
     CDS             complement(1533..>1795)
                     /locus_tag="PL106_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNA33623.1"
                     /translation="SRVLLNEVFEQLARSPQDRVLITGYTDRSGDPGTNLALSERRAR
                     AVRDYLMARGITPDRLLLNYYGDSRSSGRDPMERRVEVEWLR"
BASE COUNT          350 a          553 c          594 g          298 t
ORIGIN      
        1 gcacattgag caggatctgc gcgaggaagc cgccttcgtg gccgcgcacg acagcctgag
       61 cgatgccatg ttgcggatac gctggacccc catgctgcag agcaattggg tgctcaccgc
      121 ggttgccttg gccaacgagg cgggcgagga acagtgggtt gagcggatcg gcgacaactg
      181 gagatcgggc agcaccatcg atgcgcaggc gaacgcccag cccgtgatca tggagtggcc
      241 catgacgggg agtcccgact ccgcgcggat ccacatcgga gagccgggtc cggatcctcg
      301 tcgcgcgtta tggttcggcc aggcacttgg cgagcacacg tcgggtgcga catggagtac
      361 cgggtccgga ccggatgggt ccatggtgct gttcgcttcg atgatcatcc gttcaaacca
      421 ggaggatcgc ccctaccgcg tgatccggtt caccatctct cccgaggtcc tggtccgttc
      481 catcacggaa tcatcccagt tgtacggcat gttccacctg gaccccgaag gcagaccatg
      541 gaagctgccc gatgaccagc gatcggcagt gcccataagc agggccgtgg accagtggtc
      601 caaaacactt acggaatcgc cattctcgtt ccaacttggc gatcagcggt acatggctgc
      661 cgttcagccc aacaccatca acgggatccg cctctacacg ggaggtgtcg tggagatgga
      721 cccgatccgt gcgcgcagtc gcggggagcg catcgtgctg tggtccgcaa gcgtcctgtt
      781 ggtggtgctg ggagggctgc ttgcgggcgc atatcggcgc gggcgccagc gcgagcaacg
      841 ggctgagcgg cgcgaacggc agagcatcac acaggaacgc aggctggcca aggtgatcag
      901 cgagcgggag acactcgacc gcgaagtgca tcatcgggtg aagaacaacc tgcaagtggt
      961 gagcagcctg ttgcacctgc aggccgaacg cgtggccgac ccgggcacgc ggaacgaatt
     1021 cacccggggc aagcgtcgta tcgacagcat ggcgctggtc caccacaaac tgtatgcgca
     1081 aacagacctg cgggccatcg acctgcggct cttcatggac cagatcgcca atgctgtccg
     1141 ggccatgcac gagcccacga gccggggggt cagccacgcc gtggatacgg gagggatcac
     1201 agccaatgcc gacacggcca tccaactcgg tatgatcctc tgcgagctgc tttcgaacgc
     1261 gttcctccac gctttcccgt acatcacagg agggcacatc gacatcgagg tacgcgaggc
     1321 cgaggacggc atgtaccgga tgaccgtgcg tgacaatggc aagggcatcg aacgtcaggg
     1381 ccaggacaat ggcgccgagc tggggctcga actggccgag ggccttgcgg aacagatcga
     1441 tggccgcctg gagatcacgg tgaatgcggg cactcgggtg gatgtctact tccgcatgca
     1501 gggcgaggcg ccggtacacg gcgtcaagta ggctatcgga gccactccac ctctacccgg
     1561 cgctccatgg ggtcacgtcc tgaactgcgg ctgtcgccgt agtagttcag caggagccga
     1621 tccggggtga tgccacgcgc catcagataa tcgcgcacag ccctggcgcg gcgttcgctc
     1681 agggcaaggt tcgttccggg atccccgctg cgatcggtgt atccggtgat gagcacccgg
     1741 tcctgcggtg accgtgcgag ttgttcgaag acctcgttca acagcacccg gctcc
//
LOCUS       DAOQOC010000702         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47410_length_1794_cov_3.181139, whole genome shotgun sequence.
ACCESSION   DAOQOC010000702 DAOQOC010000000
VERSION     DAOQOC010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47410_length_1794_cov_3.181139"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..851
                     /locus_tag="PL106_10935"
     CDS             255..851
                     /locus_tag="PL106_10935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA33624.1"
                     /translation="MRTVRIKARCTILELMVEKDGVLVAEPTGWDQANVTSTLNKANA
                     ILAPARVEFSLVKFATAEFRGSRSDGRADPEADLDALAIQLGSVGFTREEVNIGFIRQ
                     FSGGGINGHSAEAWCYACIHWPFGLPQLGASVLAHELGHLLGLEHVKDQDLLMYKAAR
                     LGTPQTQLTKDEIAIIRAHPHVDHTASYAAIEKDPPRK"
     gene            complement(864..>1794)
                     /locus_tag="PL106_10940"
     CDS             complement(864..>1794)
                     /locus_tag="PL106_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNA33625.1"
                     /translation="KNGQHAHDEMLFIIIHQAYELWFKQVLYEVDSVMGIFKNKEVDD
                     NAGELNVAVHRLQRVKTILDVLVHQWEIIETMTPLDFLDFRDMLRPASGFQSMQFKIL
                     EARLGLRMEARFGKQYYTSQLKPEHKARIEVLESEQTLLELVIAWLERMPFLKPEYWP
                     GEARFFDQLEKLYVGSLVSGEEGNAAMWRKIFVDGSPERHFSPAAARSALFIMLYRDE
                     PIFQQAFRFLDSLLDIDTVMASWRARHISMVHRMIGLRVGTGGSTGKNYLKGAMDSHH
                     IFSEVADLSSFLFERRKLPALPADLKRAMGFRA"
BASE COUNT          341 a          552 c          539 g          362 t
ORIGIN      
        1 ccattggtct acgctgttgc gtaaacccat ttcaggacga gtcagtacgg acgtatccga
       61 actaccggga cggtgatgaa gttccgaagg cttggcttac cacattgctc gtggtgatgg
      121 gtgcgagagt gaggaggtgc gtgagttgta gctctcgcac cctcgcacct tcacgcggtt
      181 caacccatga tcgaccgctt cgttcccacc gtcccagtat cttcgtcctt cggtgtgacc
      241 gacccgaata tcccatgaga acagtacgta tcaaggcgcg ttgcaccatc ctggaactga
      301 tggtggaaaa ggacggggtg ctggtggccg aacctaccgg atgggaccag gcgaacgtga
      361 cctccacgtt gaacaaagcg aacgcgatcc tggcacccgc ccgtgtggag ttctccctgg
      421 tgaagttcgc gacggccgaa ttcaggggca gccgctccga tggacgagca gacccggagg
      481 cggacctcga tgcgctcgcg atccagctcg gttcggtggg cttcacacgg gaggaggtga
      541 acatcggttt catccgtcag ttcagtggtg gcggtatcaa cgggcattcg gccgaggcct
      601 ggtgttacgc ctgtatccat tggcctttcg gcctgccgca attgggagcg agcgtactcg
      661 cgcatgaact cggccatttg ctcggcttgg agcatgtgaa ggaccaggat ctcctgatgt
      721 acaaagcagc gcgcctcggc acgccacaga cccaactcac caaggacgag atcgcgatca
      781 tccgtgcgca tccgcacgtg gaccacacgg cctcgtacgc cgccatcgaa aaggatcctc
      841 cccgaaagtg atccgactgc cgctcaggcc cggaagccca tcgcgcgctt gagatccgcc
      901 ggcagcgcgg gcaacttgcg ccgctcgaag aggaagctgc tcaggtcggc cacctcgctg
      961 aagatgtggt ggctgtccat tgcgcctttc aggtagtttt ttccggtact gccaccggtg
     1021 cccacgcgca ggccgatcat gcggtgtacc atgctgatgt gccgcgcccg ccacgaggcc
     1081 atcaccgtgt cgatgtccag cagcgaatcc aggaagcgga aggcctgctg gaagatcggt
     1141 tcgtcgcgat agagcatgat gaacagggcg ctgcgggcgg ccgcgggcga gaagtggcgc
     1201 tcgggcgagc catcgacgaa gatcttgcgc cacatcgccg cgttgccctc ctctccggac
     1261 acgaggctgc ccacgtacaa tttttccaac tggtcgaaga agcgtgcttc gccgggccag
     1321 tactcgggct tgaggaaggg catgcgttcc agccacgcga tcaccagttc cagcaaggtc
     1381 tgttcgctct ccagcacctc gatccgcgcc ttgtgctccg gcttcaattg gctggtgtag
     1441 tactgcttgc cgaagcgcgc ctccatgcgc aaccccaggc gcgcctccag gatcttgaac
     1501 tgcatgctct ggaagccgct ggccgggcgc agcatgtcac ggaaatccag gaaatcgagc
     1561 ggtgtcatcg tttcgatgat ctcccattgg tgtaccagca catcgaggat ggtcttcaca
     1621 cgctgcaggc ggtgtaccgc cacgttcaac tcgccggcgt tgtcgtccac ctccttgttc
     1681 ttgaagatgc ccatcacgct gtccacttcg tacagcacct gtttgaacca gagctcatac
     1741 gcctggtgga tgatgatgaa cagcatctca tcgtgcgcgt gctggccgtt cttg
//
LOCUS       DAOQOC010000703         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47432_length_1794_cov_2.359402, whole genome shotgun sequence.
ACCESSION   DAOQOC010000703 DAOQOC010000000
VERSION     DAOQOC010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47432_length_1794_cov_2.359402"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PL106_10945"
     CDS             <1..1344
                     /locus_tag="PL106_10945"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNA33626.1"
                     /translation="HGDVAYFDAGLNERQRLWTFYEDEVNTSANLSSAFTHKFKQAGR
                     RLDIGLNYTFHREDEKYAITDQNLTGTGYNNTALIADEHVGDLKADYTKPLAHGRWEI
                     GGKFRWRVIPTRITYTAAIGSPLDQGAAGGATYNELIPALYTNYVYERERYELEAGLR
                     VEAVDLRYAVDPGHNTYTSDGYSYLEPFPNVRYARILNERSRLSISISRRVDRPDEGD
                     LRIFPKYDDPSILRIGNPDLRPQFTWRGELGYKRDLSRGYVYGAAYHRITSNILTRIV
                     TGDGTGTQLYTLAQNAGGGTNSGLEAVIEQEVVKGIRLSVNGNVYRNVIDAFSTISAY
                     PTPTPYTAKRQQTTSGNAKLNAKFRLREGMDLQLTGIWLARDIVPQGSIGERWSVDLG
                     LAWKLPGVKSELSLVATDLLNTMRIPKEVVGDGFRLVSTDYLETRAVRLTWSRIF"
     gene            1374..>1794
                     /locus_tag="PL106_10950"
     CDS             1374..>1794
                     /locus_tag="PL106_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA33627.1"
                     /translation="MKVLLVEDEPELRRSIRMYLQEEGFMVESANEFPPAMEKAQFYS
                     YDAVLVDITLPKGSGLDIVRVLRERDPATGIIIISAKGSLDDKILGLDLGADDYLPKP
                     FPLPELVARLRALIRRKQFAGKQAIELDELRIKPEEKL"
BASE COUNT          403 a          559 c          514 g          318 t
ORIGIN      
        1 cacggcgacg tggcctactt cgatgccggg ttgaacgaac gccagcggct ttggaccttc
       61 tacgaggacg aggtgaacac atcggccaac ctcagcagcg cgttcactca caagttcaag
      121 caagccggaa ggcgtttgga catcggcctc aactacacct tccaccgcga ggatgagaag
      181 tacgccatca ccgatcagaa cctcactggc accggttaca acaacaccgc cttgatcgcc
      241 gatgaacatg tgggcgactt gaaggcggac tacaccaagc cgctcgcgca cggccgttgg
      301 gaaatcggcg ggaaattccg ctggcgcgtg atccctacac ggatcaccta cacagctgcg
      361 atcggttcac cgctagacca aggtgcggcc ggtggtgcta cctacaacga gctgatcccc
      421 gcgctctata ccaactatgt ttatgaacgc gaacgctacg aactcgaagc cgggttgcgc
      481 gtggaagccg tggacctgcg ctacgccgtg gatccgggac acaacaccta caccagcgac
      541 gggtacagct atctggaacc cttccccaac gtgcgctacg ctcgcatcct gaacgaacgc
      601 agccgactgt cgatctcgat ctcccgccgc gtcgaccggc ccgatgaagg tgacctgcgc
      661 atctttccga agtacgatga cccgagcatc ctgcgcatcg gcaatcccga tctgcggcct
      721 caattcactt ggcgcggtga actcggctac aagcgggacc tgtcacgggg ctacgtatac
      781 ggggcggctt atcaccgcat cacgtccaac atcctcacgc gcatcgtaac cggcgacgga
      841 accggaacgc aactctatac cctggcgcag aacgcaggtg gcggcaccaa cagcggcttg
      901 gaggcggtga tcgaacagga agtagtgaaa ggcatacggt tgagcgtgaa cgggaacgtg
      961 taccggaatg tgatcgatgc attctccacc atcagcgctt acccgacgcc aacgccttac
     1021 acggccaagc gacaacagac caccagtggc aacgcgaaac tgaacgccaa gttccgcttg
     1081 cgcgagggca tggacctgca actcacgggt atttggctgg cgcgcgacat cgtgccgcaa
     1141 ggcagcatcg gcgagcgttg gtccgttgat ctaggcctgg cttggaagct gcccggagtg
     1201 aagagtgaat tgagcttggt ggccaccgac ctgctcaaca ccatgcgcat ccccaaggaa
     1261 gtggtaggcg atggcttccg cttggtgagc accgactatc tggaaactcg tgcggtgcgc
     1321 ctcacatggt cacgcatttt ctgaagccgg tatctcggcg atagcttgca cacatgaaag
     1381 tgctcctggt cgaggacgag ccggaactgc ggcgatccat ccgcatgtac ttgcaggaag
     1441 aaggcttcat ggtggaatcc gccaacgaat ttccaccggc gatggagaag gcccagttct
     1501 attcctacga tgcggtgctg gtggacatca ccctacccaa gggcagcggc ttggacatcg
     1561 tgcgcgtgtt gcgcgaacgg gatcccgcaa cagggatcat catcatctcc gccaagggtt
     1621 ccttggacga caagatcctc ggtctggacc tcggtgccga cgactacctg cccaaaccct
     1681 tcccccttcc ggaactcgta gcccgcttgc gcgcgctcat ccgccgcaag cagttcgcgg
     1741 gcaagcaagc catcgaactg gatgaactgc ggataaagcc cgaagagaag ttgg
//
LOCUS       DAOQOC010000704         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47681_length_1789_cov_3.106690, whole genome shotgun sequence.
ACCESSION   DAOQOC010000704 DAOQOC010000000
VERSION     DAOQOC010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47681_length_1789_cov_3.106690"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PL106_10955"
     CDS             complement(<1..194)
                     /locus_tag="PL106_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33628.1"
                     /translation="MDLRHITGKVAALSAEVAAFIRTESLRFTEANVESKGQNNLVSY
                     VDTTAEQRFVAGLSELVPQAG"
     gene            complement(197..1402)
                     /locus_tag="PL106_10960"
     CDS             complement(197..1402)
                     /locus_tag="PL106_10960"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNA33629.1"
                     /translation="MYPTLYHAFLDLFGLDIPALKFLNSFGFFVAVAFMFAHWTLSLE
                     LKRKTQQGLLRATHRKVIVGEGPKPLEMLAQGLMGFVVGWKVLYIILNIDEVTTDPPG
                     FLLSGKGNFIGGLLVGALFAYLYRRERLKSKLDKPEERTIEVPAAQHAGAITLTAALW
                     GFIGAKLFHWLENPRDFLDTLASPNAKDIVTGLTMYGGLILAGLMVMRYFRKNGLAFW
                     PGVDAAAPGVLLAYGIGRIGCQVSGDGDWGIASTLPKPSWLPGWLWSYDYPNNVNAVL
                     GPHLGGYSGVPITEGTCFPGYCTHLVPGVYPTPLYEAIACVLLFGVLWALRERIQVPG
                     VVFCLFLIFDGVERFLIEKIRVNVPWLGTWTQAEVISSLLVVGGLAGIWWFNRNRRLG
                     TANAIKDTE"
     gene            complement(1418..>1789)
                     /locus_tag="PL106_10965"
     CDS             complement(1418..>1789)
                     /locus_tag="PL106_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33630.1"
                     /translation="SERADTTAVRSSDLRKEDVVPMATRRERTQQLRILSTKLQRAHY
                     ERHLDSERPALFEAEENEGRMLGYTDNYLRVSMPYDAALAGLVVPVRLGRINGDGHIE
                     GTAVEAAHISASSHQLLGQVS"
BASE COUNT          330 a          573 c          569 g          317 t
ORIGIN      
        1 cccgcctggg gcacgagttc ggagaggccc gccacgaagc gctgctcggc cgtggtgtcc
       61 acgtagctca ccaggttgtt ctggcctttc gattccacat tggcctcggt gaaccgtaag
      121 ctctcggtgc ggatgaacgc agccacttcc gcactgagcg cggccacctt tccggtgata
      181 tgtcgcaggt ccatggtcat tcggtatctt ttatcgcatt tgcggtgccg agcctccggt
      241 tccggttgaa ccaccagatg cccgccagac cgcccacgac cagcagcgaa ctgatcactt
      301 cggcctgggt ccaggtgccc agccagggca cgttcacccg gatcttctcg atgaggaaac
      361 gttcaacacc gtcgaagatg aggaagaggc agaacacgac gccgggcacc tggatccgct
      421 cgcgcagggc ccataacacc ccgaagagca gcacgcacgc gatcgcttcg taaagcgggg
      481 taggataaac gccaggtacc aagtgggtgc aataacccgg gaagcaggtg ccctcggtta
      541 tcggcacgcc gctgtaccca cccaggtgcg gacccagcac ggcgttcacg ttgttcggat
      601 agtcgtaact ccacaaccag ccgggtagcc aggaaggctt gggcagggtg ctcgcgatgc
      661 cccagtcccc gtcgccgctc acctggcaac cgatgcgccc gatgccgtag gccagcagca
      721 cgcccggcgc ggcggcatcc accccgggcc agaaggccag cccgttcttg cggaagtagc
      781 gcatcaccat gaggccggcg aggatgaggc cgccgtacat ggtcaagccc gtaacaatgt
      841 ccttggcgtt gggggaggca agggtgtcaa ggaagtcgcg cgggttctcc agccagtgga
      901 agagcttggc cccgatgaag ccccacagcg cggccgtgag cgtgatggcc ccggcatgtt
      961 gcgcggctgg cacttcgatc gtgcgttcct cgggcttgtc cagcttgctt ttcaggcgtt
     1021 cccgccggta caggtacgcg aacagcgcgc ccaccagcaa cccgccgatg aagttgccct
     1081 tgccgctcag gaggaagccc ggcgggtctg tcgtcacctc atcgatgttc agtatgatat
     1141 agaggacttt ccagccgacc acgaagccca tgaggccctg ggcgagcatc tccagcggct
     1201 tcgggccttc gccaacgatc actttgcgat gggtggcccg cagcaggcct tgttgcgtct
     1261 tgcgcttgag ttccagcgac agggtccagt gcgcgaacat gaaggccacc gccacgaaga
     1321 agccgaagct gttcaggaac ttcagcgccg ggatgtccag gccgaacagg tcgaggaagg
     1381 cgtggtagag cgtggggtac atgctgggcg cgcccggtca gctgacctgg ccgagaagct
     1441 ggtgactgga tgcggagatg tgagcggcct cgaccgctgt cccttcgatg tgcccatcgc
     1501 cattgatgcg gccgagacgt accgggacca ccagacccgc gagcgcagca tcgtacggca
     1561 tactcacacg caggtaatta tcggtatagc cgagcatccg gccctcgttc tcctcagcct
     1621 cgaacagcgc cggtcgttcg ctgtccagat gccgctcgta gtgcgcgcgc tggagtttgg
     1681 tgcttaaaat ccggagctgt tgcgtgcgtt cgcggcgcgt ggccatgggc accacatcct
     1741 ccttccgcaa gtcggaggaa cgcacggccg tggtgtcggc gcgctcact
//
LOCUS       DAOQOC010000705         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47917_length_1785_cov_3.416763, whole genome shotgun sequence.
ACCESSION   DAOQOC010000705 DAOQOC010000000
VERSION     DAOQOC010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47917_length_1785_cov_3.416763"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PL106_10970"
     CDS             complement(<1..521)
                     /locus_tag="PL106_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA33631.1"
                     /translation="MTGPMYPGWGPEARRGLWSVLGCACLSFLTCDAPAQRSSFNNRE
                     VVPPDGTGHYRILIGGHFHGTSNNRSGYPAATLLANLDTINALAANVFVCTGDLFMDP
                     VNDRPRYVRALFSKLNMPLFNAPGNHDAPAVEDPRTTLLDIPFNVGDSGATRSDRVLI
                     LDTEQDNGSFTGD"
     gene            complement(523..>1785)
                     /locus_tag="PL106_10975"
     CDS             complement(523..>1785)
                     /locus_tag="PL106_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33632.1"
                     /translation="IYYANQLAIRKLLNVPKGFHAYNNGNRGDTLTLALVPIESLPMQ
                     VDSVLLGDGRFAAPMEPVTLPCRRVGNPGSPVEVRFLASSDSAVKVFYHIPGASTGRE
                     LEVFPFKLASEADDAKFLAAMVPNPHEFPFLAVDDSARTITIKPGAWKVDRTVVLPSG
                     YRCVATWPLELDLVNGAGIISHAALAWQGGKDQHLRIFSSDSSSSGVRVISVPSLSSI
                     VHVDFSHLTRRKGKEGRNGDVAFHASPLQMSGCTFRGTARRLIGITMGKAGIAHCSFS
                     GGSDQLEAVAAQVDVTNCLFGQAQDDAVTAEGGALTLKETALRDVAGIAVKGEKGARI
                     TADRLDVARCGVAFEGREGGKLTIANSTVADAGVAAEAKKYEMRYGPVRIELNKVVIT
                     SAGQRFKRGPESIINVDGRPAPVNDTGK"
BASE COUNT          313 a          581 c          558 g          333 t
ORIGIN      
        1 tggtccccgg tgaaggaacc gttgtcctgt tccgtgtcca ggatcaatac ccgatcggac
       61 cgtgttgccc cactatcccc gacgttgaac gggatgtcca acagggtcgt ccggggatcc
      121 tcgaccgccg gggcatcatg gttccccggc gcattgaaca agggcatgtt caacttgctg
      181 aacagggctc gcacgtatcg gggccggtca ttcaccgggt ccatgaacag atcgccggtg
      241 cagacgaaga cgttcgccgc gagcgcattg atggtatcga ggttcgcgag gagcgtggcc
      301 gccgggtacc cgctacggtt gttgctggtc ccgtggaagt gcccgccgat caggatgcgg
      361 taatggcccg taccgtcagg gggcaccacc tcgcggttgt tgaaggacga ccgttgggcc
      421 ggtgcatcgc aggtgaggaa cgagaggcaa gcgcagccga ggacggacca caggcccctc
      481 cgtgcttccg gcccccatcc gggatacatc ggcccggtca tattatttac cggtatcatt
      541 cacaggagcg ggccggccat ccacattgat gatgctctcc ggcccgcgtt tgaaccgctg
      601 ccccgcgctg gtgatcacca ccttgttgag ttcgatacgc accgggccgt agcgcatctc
      661 gtacttcttg gcctcggccg ccacacccgc atcagcgacc gtgctgttgg cgatggtgag
      721 tttgcccccc tcgcgacctt cgaaggccac gccgcaccgg gccacatcca accggtcggc
      781 cgtgatgcgt gcgcctttct cgcccttcac ggcgatgccc gcaacatcgc ggagtgccgt
      841 ctccttcaac gtgagcgcac cgccttccgc agtgacggca tcgtcctgcg cctgaccgaa
      901 caggcagttg gtcacatcca cttgcgcggc aacggcctcc agctggtcgg acccaccgct
      961 gaaggaacaa tgcgcgatgc ccgctttgcc catggtgata ccgatcaacc tgcgggccgt
     1021 gccgcggaac gtgcacccgc tcatttgaag cggtgaagca tggaaagcga catcgccatt
     1081 gcgcccctcc ttgcccttgc ggcgtgtcag atggctgaag tcgacatgga cgatcgagga
     1141 gagcgacggc acggagatca cgcgaacccc gctggaactg ctgtcgctgc tgaagatccg
     1201 gaggtgctga tccttgccac cctgccaggc cagcgccgca tggctgatga tgcccgcccc
     1261 gttcaccagg tcgagttcga gcggccacgt ggccacgcag cggtatccgg agggcagtac
     1321 caccgtgcgg tccactttcc aggcacccgg cttgatggtg atggtgcgcg cactgtcgtc
     1381 cacggccagg aaagggaatt catgcgggtt cggcaccatt gccgcaagga acttcgcgtc
     1441 gtccgcctcg ctggcgagtt tgaacgggaa aacctccagt tcgcgcccgg ttgaagcacc
     1501 cggaatgtgg tagaaaacct tcacggcgct gtcgctgctg gccaagaacc gcacttccac
     1561 gggtgatccc gggttcccga cccttctgca cggcagggtg accggctcca tcggggcagc
     1621 aaagcggcca tcacccagca gcacgctgtc cacctgcatg ggcaggcttt cgatgggcac
     1681 gagcgcaagg gtgagcgtgt cgccccggtt cccgttgttg tacgcatgga accctttcgg
     1741 cacgttgagc agcttgcgta tggccagctg gttggcgtag tagat
//
LOCUS       DAOQOC010000706         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_47986_length_1784_cov_2.451128, whole genome shotgun sequence.
ACCESSION   DAOQOC010000706 DAOQOC010000000
VERSION     DAOQOC010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47986_length_1784_cov_2.451128"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="PL106_10980"
     CDS             complement(<1..1556)
                     /locus_tag="PL106_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNA33633.1"
                     /translation="MNRADRIFKTSEGNMHKGSRIIGAAAVLLSTQLSAQTITFLDEA
                     TRAPVPDVPITCVGTGRTITSMADGRADISSLKGCGRLLVDHLAYKPITFDWAALADT
                     PVVNLSYRLNQLREVVTSGSRFNEARADVPEKIDVLSRREIGFMAQPTAAELLQNTGT
                     VFVQKSQLGGGSPTIRGFQANRVLLVVDGVRMNNAITRSGHTQDLITVDQYALDRVEV
                     VSGPNSVAYGSDALGGTIHLITRSPKFLGRDSIRTAGDAFLRFSTAAMEKTAHAGIEL
                     RGRDLTSFTSITASDFGDLRQGSTRNPFYEDLGRMPFHVVRENGQDVVRTNDDSNVQL
                     GTGYKQIDVLEKLRLRSGDHLIHQLNLQLSTSSNVPRYDRLSAYATDSTGGIIPTYAE
                     WFYGPQQRVLAAYTAEVDKEATWFNSARITPSWQHQEQSRYNRRFQSNKRGINIEKVD
                     VFGCNVDLEKRIGKHEIRYGLNLSHETVNSTAWNENISTGEHSYRTTRYPNGGSTMDN
                     LAAYVNHSME"
BASE COUNT          277 a          568 c          558 g          381 t
ORIGIN      
        1 atctccatgc tgtggttcac gtacgcggcc aggttgtcca tggtggaacc tccattgggg
       61 tagcgtgtgg tccggtagct gtgctcaccg gtgctgatgt tctcgttcca ggccgtgctg
      121 ttcacggtct catggctcaa gttcaacccg tagcggatct cgtgcttgcc gatgcgcttc
      181 tccaggtcca cgttgcagcc gaagacgtcg accttctcga tattgatccc ccgcttgttg
      241 ctctggaagc gacggttgta ccgcgattgc tcctggtgct gccacgaggg tgtgatgcgg
      301 gcgctgttga accaggtcgc ttccttgtcc acctcggcgg tgtacgcggc cagcacccgt
      361 tgctgcgggc cgtagaacca ttccgcgtag gtcggaatga tcccgcccgt gctgtccgtg
      421 gcgtacgcgg agaggcggtc gtagcgcggc acgttcgagg atgtgctcaa ctgcaggttc
      481 agctggtgga tgaggtgatc gccggagcgg agccgcaact tctccagcac atcgatctgt
      541 ttgtatcccg tgccgagctg cacgttgcta tcgtcgttcg tccgcaccac gtcctgacca
      601 ttctcgcgca ccacatggaa cggcatgcgt ccaaggtctt catagaacgg gttccgtgtg
      661 ctgccctgcc gcaggtcgcc gaagtcgctg gcagtgatgc tggtgaagct ggtgaggtcg
      721 cgtccgcgca attcgatacc cgcatgggcc gtcttctcca tggccgcggt gctgaagcgg
      781 aggaacgcat ctccagcggt gcgtatgctg tcgcgcccca agaacttcgg gctgcgggtg
      841 atcaagtgga tggtgccccc caaggcatcg ctcccgtacg ccacggaatt gggtccgctc
      901 accacctcca cgcggtccag cgcatactga tccacggtga tcaggtcctg ggtgtgcccg
      961 cttcgcgtga tggcgttgtt catgcgcaca ccatcgacca ccagcagcac gcggttggcc
     1021 tggaagccac ggatggtggg acttccacca ccgagctggc ttttctgaac gaagacggtg
     1081 cccgtgttct gcagcagttc ggccgcggtc ggttgggcca tgaaaccgat ctcccgtctc
     1141 gaaagcacgt cgatcttctc gggaacatcc gcccgggcct cgttgaagcg gcttccactg
     1201 gtgaccactt cgcgcaactg gttcaagcgg tagctcaggt tcaccaccgg cgtatcggcc
     1261 agtgccgccc agtcgaaggt gatgggcttg taggccaggt gatccaccag gagcctcccg
     1321 cagcccttca agctgctgat gtccgcgcgc ccgtcggcca tggacgtgat ggtccggccc
     1381 gtgccaacgc aggtgatcgg aacatcgggc accggtgcgc gcgtggcctc gtccaggaag
     1441 gtgatggtct gggcactgag ttgtgtactg agcagtaccg cagccgctcc gatgatcctt
     1501 gatcccttgt gcatgttccc ttcgctggtc ttgaagatcc gatcggcccg gttcatggga
     1561 gggggccaac accaccgcac gcgctcgtgc ccgggccctg gtacttcgtt cgactggtcg
     1621 gctttcggct cgcctgcgaa taaacggctc aaccgccgaa ctcccgtacc cgagcggtcg
     1681 cgtccatgtt ctggcaccca agttgactgc ccccgtccgg ccgggcgacc agcacgcgtg
     1741 cgatccgtcc ttcagcgacc gttctcgtgc gacagggcgg atac
//
LOCUS       DAOQOC010000707         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48093_length_1782_cov_3.380428, whole genome shotgun sequence.
ACCESSION   DAOQOC010000707 DAOQOC010000000
VERSION     DAOQOC010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48093_length_1782_cov_3.380428"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="PL106_10985"
     CDS             complement(<1..1762)
                     /locus_tag="PL106_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA33634.1"
                     /translation="MSSHDIEKDLHGPERQFGITTWAVNNRTTVIVLTILICLLGIRA
                     YIVMPKVSFPDVVTPEVFIGTPYNSSSVVDIEKLITKPIEKELNTISGVDEIKSTSVP
                     NFSSIHIKFDYSVTPTEALRKVKDAVDKARGDSDFPTDLPSEPNVFEMNFSELIPVMN
                     INLSGDYPLEQLHEYAKHLEDRIEELPEINKVEIRGVPEREVKVSVDLAAIEMLQLNF
                     DDIANALRSENLTMSGGDILTKDQRRSVRVIGEFTGMEQIRNIVVKNEGQREVRLRDV
                     ADVSFGFKEPESFAREFGKSVVMLDVIKRAGENLLDASDKINAIIQEDHGTVLPKDLT
                     LTITSDQSDQTRVSVDELMNHIVLGVILVVGVLMLFLGLRNAVFVGLAIPMSMFISFT
                     LLNAFGVTLNMMVLFALILALGRLVDDGIVIVENIHRHMSNGEPALKATKLAVGEVTM
                     PIIAATTATVMVFVPLLFWPGMMGEFMKYLPITFMIALASSLFVALVVNPALASKFMK
                     VEEDHMPTGKMWRTVLVLAIAGAVIGVLGASMKSNAVFGIGMLAIFFGLMGIANQKLF
                     APAAHWFQYTWLPRLETRYRR"
BASE COUNT          314 a          528 c          566 g          374 t
ORIGIN      
        1 accgccgata gcgggtttcc aagcggggca gccaggtgta ctggaaccaa tgggccgccg
       61 gggcgaacag cttctggttc gcgatgccca tcaggccgaa gaagatggcg agcatgccga
      121 taccgaaaac ggcattgctt ttcatggacg ctccgagcac gccgatcacc gcacctgcga
      181 tggccagtac cagcaccgtt cgccacatct tcccggtcgg catgtggtcc tcctccacct
      241 tcatgaattt ggaggcgagc gccgggttca cgaccagcgc cacgaagagc gagctggcca
      301 gcgcgatcat gaaggtgatg ggcaggtact tcatgaactc gcccatcata ccgggccaga
      361 agagcagggg gacgaagacc atcacggtgg ccgtggtggc ggcgatgatc ggcatggtca
      421 cttcgcccac ggcgagtttg gtggccttga gcgcgggttc cccgttgctc atgtggcggt
      481 ggatgttctc cacgatcacg atgccgtcgt cgaccagtcg cccgagcgcg aggatcagcg
      541 cgaagagcac catcatgttc agcgtgacac cgaacgcgtt gagcagggtg aaggagatga
      601 acatgctcat ggggatggcg aggcccacga agaccgcgtt ccgcagaccg aggaagagca
      661 tgagcacgcc caccaccagg atcacgccga gcacgatgtg gttcatcagt tcgtccacgc
      721 tcacgcgggt ctggtcgctc tggtcgctgg tgatggtgag ggtgaggtcc ttgggcagca
      781 cagtgccgtg gtcctcctgg atgatggcgt tgatcttgtc gctggcatca agcaggttct
      841 cgccggcccg cttgatcaca tcgagcatca ccacgctctt gccgaactcg cgtgcgaaac
      901 tctcgggctc cttgaagccg aagctgacat cggccacatc gcgcaggcgc acttcgcgct
      961 ggccctcgtt cttcaccacg atgttgcgga tctgctccat gcccgtgaat tccccgatca
     1021 cgcgaacgct gcgccgctgg tccttggtga ggatgtcccc gccgctcatg gtgaggttct
     1081 cgctgcgcag cgcgttggcg atgtcgtcga agttcaactg cagcatctcg atggcggcga
     1141 ggtccacgct caccttcacc tcgcgctcgg gcacgccgcg gatctccacc ttgttgatct
     1201 ccgggagttc ttcgatgcgg tcctccaggt gcttggcgta ctcgtggagc tgctccagcg
     1261 gatagtcgcc gctgaggttg atgttcatca ccggtatcaa ttcggagaag ttcatctcga
     1321 acacgttcgg ttcgctcggc aggtccgtgg ggaagtcgct gtcgccacgc gccttgtcca
     1381 ccgcgtcctt caccttgcgc agggcttcgg tgggcgtaac gctgtagtcg aacttgatgt
     1441 ggatggaact gaagttgggg acgctggtgc tcttgatctc gtcgacgccg gaaatggtgt
     1501 tcagctcctt ctcgatcggc ttggtgatca gtttctcgat gtcgaccacc gagctgctgt
     1561 tgtacggcgt accgatgaac acctcgggcg tgaccacgtc cggaaagctc accttgggca
     1621 tcacgatgta ggcgcggatg ccgaggaggc agatgaggat ggtgagcacg atcacggtgg
     1681 tgcggttgtt caccgcccag gtggtgatgc cgaactggcg ttccggtccg tgcaggtctt
     1741 tctcgatgtc gtgtgagctc atgctggaat gcttttcacc ac
//
LOCUS       DAOQOC010000708         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48105_length_1782_cov_2.767805, whole genome shotgun sequence.
ACCESSION   DAOQOC010000708 DAOQOC010000000
VERSION     DAOQOC010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48105_length_1782_cov_2.767805"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1782
                     /locus_tag="PL106_10990"
     CDS             16..>1782
                     /locus_tag="PL106_10990"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA33635.1"
                     /translation="MTGATTVLTTSNDDSPSGLQNIGFTFLFNGVNYTQWSCTPDGFL
                     KLGTPVATADFSNGISENTNMPKIMPYYDDMATGTTGNVTSVVTGTAPNRILVVQWFV
                     TIPRATGGAANSTFQCWLHETTGAIEFHYGTMGPGAMSASVGIAASTTNYQSVTITGA
                     TVSTVAANDVNGDQPASGTMYSFTNPPPASLLWTPNTFLNFDNIANPMASGVNVASQA
                     YTVTATASNGCTSTGNVTVNTSAPITAASITGTLAFCTGGSTTLTAVPADGGGPFTYL
                     WSPGGETTASIVVTTAGSYSCQVSDACGGSVNTGSVSVVENFLPTVVVDVPSANYCTG
                     NPSIAITASGASTYAWSPAAGLDATNVAAVNANPTSSTTYTVTGTDGNGCQNTATSAI
                     TVLGAIPVINSTTATPPTVCIGGNSQLQVNVPGPGSYCNSNFTSVTYEFLTNVTFGGI
                     NNTSAGNAGGPVDYTAQVASVTAGTSYNLDVTMDPDALDYIYAFFDWNQNGVLNDVGE
                     AFTVVASSNVAGPHTISIPVPANATNGNTRMRVMVDWSNSTPNPCRIANFGEAEDYTV
                     NVSGGNDAYTFSWTPATYLSSTT"
BASE COUNT          370 a          609 c          524 g          279 t
ORIGIN      
        1 ggcagccttg acccgatgac cggcgcaacg acggtgctta caacgagcaa cgacgacagc
       61 ccgagcggct tgcagaacat cggcttcacc ttcctgttca acggggtgaa ctacacgcaa
      121 tggtcctgca cgcccgatgg cttcctgaag ttgggaaccc cggtagcaac ggctgacttc
      181 agcaatggca tttcggagaa taccaatatg ccgaagatca tgccttacta cgacgacatg
      241 gccaccggca ctaccgggaa cgtgacgtcg gtggtaaccg gtacggcccc gaaccggatc
      301 ctggtggtgc agtggttcgt gacgatcccg cgcgccacgg gcggcgcggc gaacagcacc
      361 ttccagtgct ggttgcatga gaccactggt gccattgaat tccattacgg aaccatgggg
      421 cccggtgcca tgtccgcctc agtgggtatc gccgcttcga cgaccaacta ccagagcgta
      481 acgatcacgg gggccacggt tagcacggtg gcagcgaacg atgtgaacgg ggaccaaccg
      541 gcctcgggca cgatgtacag cttcacgaat ccgcccccgg cctccctcct ctggacgccg
      601 aacaccttcc tgaacttcga caacatcgcc aacccgatgg cctccggcgt gaacgtggcc
      661 agccaggcgt acactgtgac cgcgacggcc tccaacggtt gcacgagtac cggcaacgtg
      721 acggtgaaca cctcggcccc catcacggcg gcgagcatca ccggcaccct ggccttctgc
      781 acgggcggca gcaccacctt gacggccgtt ccggccgatg gtggtggccc cttcacctac
      841 ctgtggagcc ccggcgggga gaccacggcc agcatcgttg tgaccaccgc cggcagctat
      901 agctgccagg ttagcgatgc ctgcggcggc agtgtgaaca ccggcagcgt gagcgtggtg
      961 gagaacttcc tgcccaccgt ggtggtggat gttccttccg ccaactactg cacgggcaac
     1021 ccgtcgatcg ccatcacggc aagcggcgcc agcacctatg cctggagccc ggccgcaggc
     1081 ctggatgcca cgaacgtggc cgctgtgaac gccaacccca ccagcagcac cacctacacg
     1141 gtgaccggca cggacggtaa cggctgccag aacacggcca ccagcgcgat caccgtgctg
     1201 ggcgcgatcc ccgtgatcaa cagcaccacg gccaccccgc caaccgtatg catcggtggc
     1261 aactcgcaat tgcaggtgaa cgtcccggga ccgggttcgt attgcaacag caacttcacg
     1321 agcgtgacct acgagttcct cacgaacgtg accttcggcg ggatcaacaa caccagtgcg
     1381 ggcaacgcgg gcggccccgt ggattacacc gcgcaagtgg ccagcgtgac cgccggaacc
     1441 tcctacaacc tggatgtgac gatggatccc gatgcgctcg actacatcta cgccttcttc
     1501 gactggaacc agaacggtgt gttgaacgat gtgggcgaag ccttcaccgt ggttgccagc
     1561 agcaacgtcg caggcccgca cacgatcagc atcccggtgc ccgccaacgc cacgaacggc
     1621 aacacgcgca tgcgtgtgat ggtggactgg agcaactcca cgcccaaccc ctgccgcatc
     1681 gccaactttg gtgaagcgga ggattacacg gtgaacgtga gcggcggcaa cgacgcttac
     1741 acctttagct ggacccctgc cacctacctg agcagcacca cc
//
LOCUS       DAOQOC010000709         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48106_length_1782_cov_2.749855, whole genome shotgun sequence.
ACCESSION   DAOQOC010000709 DAOQOC010000000
VERSION     DAOQOC010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48106_length_1782_cov_2.749855"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PL106_10995"
     CDS             complement(<1..552)
                     /locus_tag="PL106_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA33636.1"
                     /translation="MFLRRKTIEPLSDEDLVERVRSGQRTSLAALWDRYAHLLFGVGM
                     KYLKDTEGAKDLVVEVFASLPELLGKHEVKSFRPWIHTVMRNRCLMLLRKDDPERRVD
                     GSLLGAPDEMNDDAVLHEATLQRLEAAVGELKDAQRTCIRLFYLERNSYQQTAERTGF
                     SVEQVRSHIQNGRRNLRLILERDD"
     gene            complement(615..1337)
                     /locus_tag="PL106_11000"
     CDS             complement(615..1337)
                     /locus_tag="PL106_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33637.1"
                     /translation="MGTRIGITVAVMALSAAASGQGLGSIEGRLLEESGEALPFTSVS
                     ATLGTKVYTATSDPDGWFRLKPLQPGEYEVRVVAMNMDLTKDGILVLPDNITRMGDLV
                     LKNAKDLPAVVVVKERWIAPLIDHDNTGVTKVSHKLFENNPNKVAPVRLIASFAPGVV
                     KAPNGDGLYFRGARAENMCYYVDGMKLGSTLSGVPNEAINSFSVYTGGVPAKYGDVTG
                     GIVAIETKSYFDLYQQHNPGVR"
     gene            complement(1404..>1782)
                     /locus_tag="PL106_11005"
     CDS             complement(1404..>1782)
                     /locus_tag="PL106_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNA33638.1"
                     /translation="VDWDLVQLELREYIQDYLNTDGRVVNSDAPAQQLADANERAVTH
                     AEATTDDERRIIAVIDEYVSPAVAGDGGHIAFRSYKDGVVAVDLRGSCSGCPSSMVTL
                     KGGIENLLKQMVPGVREVVAVEL"
BASE COUNT          290 a          602 c          525 g          365 t
ORIGIN      
        1 gtcatcgcgt tcaaggatga ggcgcaggtt gcgacgcccg ttctggatat ggctgcgcac
       61 ctgctccacg gagaagcccg tgcgttcggc cgtctgttga tagctgttcc gttcgaggta
      121 gaataatcgg atgcaggtcc gctgggcatc cttcagttct cccaccgcgg cttcgaggcg
      181 ttgcagggtg gcctcatgca acacggcatc gtcgttcatc tcatccggag cgccaagcag
      241 cgacccgtcc acgcgccttt cgggatcgtc cttgcgcaac aacatcaagc agcggttgcg
      301 catcaccgtg tggatccatg gcctgaacga cttcacctca tgcttgccca acagttccgg
      361 cagcgatgcg aagacctcga ccacgaggtc cttcgcgcct tcggtatctt tcaagtactt
      421 catgccgaca ccgaagagca agtgcgcgta acggtcccac aacgcggcga gcgaggtccg
      481 ctggccgctc ctcacccgct ccaccaggtc ctcgtcgctc aggggttcta tggtctttcg
      541 ccggaggaac atgctcgtga gggtccgcgg ggctgttgtc agcaacgtgc gctgcgcctc
      601 gcacctggca ccgcctaccg gaccccaggg ttgtgctgct ggtacaggtc gaagtaactc
      661 ttcgtttcga tagcgacgat accgccggtg acatcgccgt atttcgccgg tacgccaccc
      721 gtgtacacgc tgaagctgtt gatggcctcg ttggggacgc cgctcaaggt gctgccgagc
      781 ttcatgccgt ccacgtagta acacatgttc tcggcgcgtg cgccccggaa gtacaggccg
      841 tcgccgttgg gcgccttcac caccccgggc gcgaaggagg cgatcagtcg caccggggcc
      901 accttgttgg ggttgttctc gaagagcttg tgcgagacct tggtgacgcc cgtgttgtcg
      961 tgatcgatca gcggtgcgat ccaccgctcc ttcaccacca ccaccgcggg caggtccttg
     1021 gcgttcttca gcaccagatc ccccatgcgc gtgatgttat ccggcaatac gaggatgccg
     1081 tccttggtga ggtccatgtt catggccacc acccgcacct cgtactcgcc cggctgcagg
     1141 ggcttcagcc tgaaccagcc gtccggatcg ctcgttgccg tgtacacctt ggtgcccagc
     1201 gtggcactca cactggtgaa gggcagcgct tccccgctct cttccaacag gcgcccctcg
     1261 atgctgccga gtccctgccc cgaagcggcg gcgctgagcg ccatgaccgc caccgtgatc
     1321 ccgatccgtg ttcccatgat gttgcggcca ttggtcggcc gttcacacag gagatgcggc
     1381 gctgggccgg aatgcataac cgcctacaac tccacggcca ccacttccct cactccaggc
     1441 accatctgct tgagcaggtt ctcgatgccg cccttgaggg tgaccatgct gctggggcag
     1501 ccgctgcacg aaccccggag gtccacggcc accactccgt ccttgtatga gcggaaggcg
     1561 atgtggccgc catcgcccgc aaccgccggg ctcacgtact cgtcgatcac ggctatgatc
     1621 ctccgttcgt cgtccgtggt ggcctcggca tgggtgacgg cccgctcgtt ggcgtcggcc
     1681 agttgctggg ccggagcgtc ggaattaact acacgtccat cggtattcag gtagtcctgg
     1741 atgtactcgc gcaactccag ttgcaccagg tcccagtcca ct
//
LOCUS       DAOQOC010000710         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48161_length_1781_cov_2.789108, whole genome shotgun sequence.
ACCESSION   DAOQOC010000710 DAOQOC010000000
VERSION     DAOQOC010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48161_length_1781_cov_2.789108"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PL106_11010"
     CDS             complement(<1..850)
                     /locus_tag="PL106_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33639.1"
                     /translation="MVYGLLFSGLLLGSFQEPVVLVTTAPDLASSVAQLDRIRDAGMH
                     VRVVTGPGVFFGTLEENASTDMLMRMGAIIRAEETTDRHGSSIAQDLALDYLAALEEG
                     RFEPATTRGPMDWDGHPDHQLSRSEAEHGGGDREAQTAPDWTCSNSYNSEYMAGTVYV
                     GAFFVESNGVVDTDQYTWTPQAVSDVELQLLDAWSIWSWTAQQHGVTVTAVLELYGPG
                     TSTTLQPYEPVLHPSTEDHLWIGAIMGNLGFSGSGHMAACDAFNSSKRLALNATHAYC
                     SFIAYNP"
     gene            1027..>1781
                     /locus_tag="PL106_11015"
     CDS             1027..>1781
                     /locus_tag="PL106_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNA33640.1"
                     /translation="MLTLDQLDLSVFLTAHGWIQLLTLTVLEIVLGIDNIIVISILSG
                     ELPKERQRGARRMGLALAMITRLLLLLTLNWLTGLKEPLFSLGPIVFDGRSIVLLLGG
                     LYLLYKSAKEIHGTVELVDHREGTHRKTAVFWAVVMQIVLIDIVFSLDSVITAVGMSN
                     EILIMMLAVIIAVLIMLVASDAISTFVNKHASVKVLALAFLFIVGVVLVLDGFAHQWA
                     HDNHIKNYAYGAMAFSVAVEVLKAVGLVGSPLQ"
BASE COUNT          325 a          557 c          533 g          366 t
ORIGIN      
        1 gtgggttgta cgcgatgaag ctgcagtacg catgcgtggc gttcaaggcg agccgcttgg
       61 aggagttgaa cgcgtcgcat gcggccatgt gcccggatcc actgaaaccc aggttgccca
      121 tgatcgcgcc gatccacagg tggtcctccg tactcggatg caggaccggt tcgtagggct
      181 gcagcgtggt gctggtaccg ggcccataca gttccaggac cgcggtcacc gtgacgccgt
      241 gctgctgagc ggtccaactc caaatactcc aagcgtccag gagttgcagt tcaacgtcgc
      301 tcacagcttg tggggtccag gtgtactggt cggtgtccac caccccgttg ctctctacga
      361 aaaaagcgcc gacgtacacg gtaccggcca tgtactcgct gttgtagctg ttggaacagg
      421 tccagtccgg tgcggtttgc gcttcgcgat cgccgcctcc atgttccgct tccgatcggc
      481 tcagctggtg gtcgggatga ccgtcccaat ccatcgggcc cctggtcgtt gctggttcga
      541 accggccttc ctcaagggct gccaggtaat ccaacgccag atcttgcgcg atcgatgatc
      601 cgtggcggtc ggtggtttcc tctgccctga tgatcgcgcc catccgcatc agcatgtccg
      661 tggatgcatt ctcctcaaga gtgccgaaga acacaccggg gcctgtgacc acacgcacgt
      721 gcatgcccgc atcccggatc ctgtccaact gggccacgct gctcgcgagg tccggtgctg
      781 tggtaacgag cacgaccggt tcctggaacg acccgagaag aagccccgag aacaggaggc
      841 catagaccat caggatcgat cgtgaaatga acataccggg gaaaagggca tcgaaagtag
      901 gccgatccag ggatcccgaa ggcccacgcc agggtccttt ttcccctccc cggatccgaa
      961 gtgggaggcc cggatatctt cgccgcccgc aagtcggaac cgcatggccc gaccggcctt
     1021 tcgctgatgc tgacactcga ccagctcgat ctatcggtct tcctcacggc ccacggctgg
     1081 atccaactcc tcaccctcac ggtgctggag atcgtgctgg gcatcgacaa catcatcgtc
     1141 atcagcatcc tcagcggaga actgcccaag gaacggcaac gcggtgcccg tcgcatgggc
     1201 ctggccctgg ccatgatcac ccgcctgctg ctgttgctca ccctcaactg gctcaccggc
     1261 ctcaaggagc cgctcttcag cctgggtccc atcgtcttcg acggccgttc catcgtgctg
     1321 ttactgggcg gcctgtatct gttgtacaag agcgccaagg agatccatgg cacggtggaa
     1381 ctcgtggatc atcgcgaggg cacccatcgg aagaccgccg tgttctgggc cgtggtgatg
     1441 cagatcgtgc tcatcgacat cgttttctcc ctcgattcgg tgatcacggc ggtgggcatg
     1501 agcaacgaga tcctgatcat gatgctggcg gtgatcatcg cggtgctcat catgctggta
     1561 gccagcgatg cgatcagcac ctttgtgaac aagcatgcca gcgtgaaggt gctggccctg
     1621 gccttcctct tcatcgtggg tgtggtgctg gtgctggacg gtttcgcgca ccaatgggca
     1681 cacgacaatc acatcaagaa ctacgcgtac ggtgccatgg ccttcagcgt ggccgtggaa
     1741 gtgctcaaag ccgtcggttt ggtcgggtcc cccttgcaga t
//
LOCUS       DAOQOC010000711         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48186_length_1780_cov_4.565797, whole genome shotgun sequence.
ACCESSION   DAOQOC010000711 DAOQOC010000000
VERSION     DAOQOC010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48186_length_1780_cov_4.565797"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PL106_11020"
     CDS             complement(<1..1257)
                     /locus_tag="PL106_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33641.1"
                     /translation="MNGQATLTNLCLFLLALGSAPARAQFTVGGQVLQRSEFRNGYGR
                     LIDDTTQTPAFFIGHRARIHALYQHEKVRFHVSVQDVRPWGSTEQGKATDDFLSVHEA
                     WGELIFNQQWNLKLGRQELAYDGNRFLGGADWALPGRAHDFALLRYEKGKTKVHAGGG
                     YNVAAESLTDQPYTVPKQYKTAQMLRLEQQWGAFTVSAFLWNNGLEQQQLDSLGEVTK
                     RTMRYSQTIGLPTIKYERKRFMANAYGYFQVGQDRSGNDMQAYDASAWVTYTVPMDTT
                     KGSAFRMTLGAEVLSGTAQDATDKVNRSYAPLYGTAHAFNGYMDHFYVDGRWGNSVGL
                     TDIFLRLRYDIDKRTLLSMNLHEFQAAADVIKNGERLDPRLGNEVDLSVVHLLNDVVA
                     LQFGYSQLFATGTLEHMEGVIDPAALQ"
     gene            complement(1274..1636)
                     /locus_tag="PL106_11025"
     CDS             complement(1274..1636)
                     /locus_tag="PL106_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA33642.1"
                     /translation="MSSKKKDDPPKPYARFKDRFPRIAERYEALGDAVHDHGPLNDRE
                     RALLKLAISGSAVLDSAFKTHVRKARALGIGRAEIEHVALLFLPTRGLPVMMRALQLV
                     DDMYKEDPGPRSGGRSKT"
BASE COUNT          301 a          543 c          557 g          379 t
ORIGIN      
        1 ctgcaaggca gccggatcga tcacgccttc catgtgttcc agtgttccgg tggcgaacaa
       61 ctgggaatag ccgaactgca gtgcgaccac atcgttgagc aggtgtacca cgctcaggtc
      121 gacctcgttg cccaaccggg ggtcgaggcg ttcgccgttc ttgatcacat cggccgcggc
      181 ctggaactcg tgcaggttca tcgagagcaa cgtgcgcttg tcgatgtcgt accgcaaacg
      241 caggaagatg tccgtcagac ccacgctgtt cccccatcga ccgtcaacat agaagtggtc
      301 catgtatcca ttgaaggcgt gagcggtgcc atagagcggg gcgtaggacc ggttcacctt
      361 gtcggtggcg tcctgcgccg tgcctgaaag cacttcagca cccagcgtca tccggaaggc
      421 ggatcccttg gtcgtgtcca tcggtacggt gtaggtgacc cacgcgctgg catcataggc
      481 ctgcatatcg ttgcccgatc ggtcctggcc cacttggaag tagccgtacg cgttggccat
      541 gaaccgcttg cgttcgtact tgatggtggg cagaccgatg gtctgtgagt agcgcatggt
      601 gcgcttggtc acctcgccaa ggctgtcgag ctgttgctgt tccagtccgt tgttccatag
      661 gaaggcgctc acggtgaacg cgccccattg ctgttccagc cgcagcatct gcgcggtctt
      721 gtattgcttg ggaacggtgt aaggctggtc ggtgaggctt tcggcggcca cgttgtaacc
      781 acctccagca tgcaccttgg tctttccctt ctcgtaccgc aggagcgcga agtcgtgcgc
      841 ccgacccggc agcgcccaat ccgcgccgcc cagaaagcgg ttgccgtcgt aggccagttc
      901 ctgccgaccc agcttgaggt tccactgctg gttgaagatc agttcgcccc aggcttcatg
      961 cacggagagg aagtcatccg ttgctttccc ctgctcggtg ctgccccagg gtcgcacgtc
     1021 ctgcacgctc acgtggaagc gcactttctc gtgttgatag agcgcgtgga tgcgcgcgcg
     1081 atggccgatg aagaacgccg gggtctgcgt cgtgtcatcg atcagcctac cgtacccgtt
     1141 gcggaactcg ctgcgctgaa ggacctggcc tccaacggtg aactgggccc gcgccggcgc
     1201 gcttcccaat gcgaggagga acaggcagag gttcgtcagc gtggcttgtc cgttcatggt
     1261 ccgcatgatg ggttcaggtc ttggaccggc ctcccgatcg cgggcccgga tcctccttgt
     1321 acatgtcgtc caccagttga agcgcgcgca tcatcaccgg gagcccgcgg gtgggcagga
     1381 agagcagggc cacatgttcg atctccgcgc ggccgatgcc cagtgcgcgt gccttgcgca
     1441 cgtgggtctt gaacgcgctg tcaagcaccg cgctgccgga aatggccagc ttcagcaatg
     1501 ctcgttcacg gtcgttcagc gggccgtggt catgcacggc gtcgcccaac gcctcgtacc
     1561 gttcggcgat ccggggaaat cgatccttga accgagcata gggcttgggc gggtcatcct
     1621 tcttcttgct gctcatggtg cgtggttttg atcctcccgg atgggtctga tttcaacacg
     1681 ggcaggcaca gcccttcttg ttgtagaagt tccagttcac ccaggtggct gccagcagga
     1741 agaccagcat caggccggtg atccatgtaa gagtacgacg
//
LOCUS       DAOQOC010000712         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48191_length_1780_cov_3.928696, whole genome shotgun sequence.
ACCESSION   DAOQOC010000712 DAOQOC010000000
VERSION     DAOQOC010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48191_length_1780_cov_3.928696"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PL106_11030"
     CDS             <1..311
                     /locus_tag="PL106_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNA33643.1"
                     /translation="YDLVLYVANWPEARRHPWSTLLLARAIENQAYVAGVNRTGMDGK
                     GLHYSGDSVIIDPKGEVIAAVDPSRDGIKTARLDMAALNDFRAKFPVAQDADGFDLLN
                     "
     gene            complement(308..>1780)
                     /locus_tag="PL106_11035"
     CDS             complement(308..>1780)
                     /locus_tag="PL106_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33644.1"
                     /translation="NAFSWSTGANDQQITVTQDGTYSVSVTTPFNCTAADEVNIAFHA
                     LPTDELADATVCAGTPVSLNAGNPGASYQWNNGATTQSIVPDSSGTYSVTIITPEQCA
                     ATYVAIVAILPVVNIDLGPDTTLCSGQVLVLDAGTSGLNYTWNTGATTQTIQATTSGT
                     YSVSASNGACAGQDAISLTFDPSPTDPLQDTIACAGGTITLDAGNPGCTWLWNTGATT
                     PTIDVVSSGTYSVTVTNAMNCSGHFDAEVTFADPPVVDLGPDTVLCEGDVLTLDAGNP
                     GATYQWSNGQDTRTINVTSGGIYSVVVNNGLCTGTDGISTTFNQRPDRLGTHQFFTCL
                     DKEPHYVVISAGNNGSSYEWSTGASSQVILAGAYGWYYVDITNSLDCSVRDSAVVTEF
                     CDPSLYVPNSFTPNGDGVNDIWHADGNNIGYFNLNVFDRGGQVIFHSENFTYGWDGKI
                     DGEDAPPGVYVWRMEYRFVKDTEGKEGMIHSQLGHVTLLR"
BASE COUNT          325 a          517 c          591 g          347 t
ORIGIN      
        1 actacgacct ggtgctgtat gtggccaact ggcccgaagc acggcggcat ccatggagca
       61 cgctgttgct ggcgcgggcc atcgagaacc aggcgtacgt ggcgggcgtc aaccgcaccg
      121 ggatggacgg caagggcctg cactacagtg gcgacagcgt gatcatcgac cccaagggag
      181 aggtgatcgc cgcggtggat ccctcacggg atggtatcaa gaccgcgagg ctggacatgg
      241 ccgcgctcaa tgacttccgg gcgaagttcc cggtggcaca ggacgccgac ggtttcgatc
      301 tgctgaacta acggagcagg gtcacgtgcc cgagttggct gtggatcatg ccctcctttc
      361 cctccgtgtc cttcacgaag cggtattcca tgcgccacac ataaacaccg ggaggcgcgt
      421 cctcgccatc gatcttgccg tcccatccgt acgtgaagtt ctcgctgtgg aagatgacct
      481 ggcccccgcg gtcgaagaca ttgaggttga agtagccgat gttgttgcca tcggcgtgcc
      541 agatgtcatt caccccatca ccgttcggcg tgaatgaatt gggtacatac aaactgggat
      601 cacagaattc cgtgaccacg gcactgtcgc gcacggaaca atcgagggaa ttggtgatgt
      661 cgacataata ccaaccgtac gcgccggcga ggatcacctg cgagctggcc ccggtgctcc
      721 attcgtacga ggagccgttg ttgcccgcgc tgatgaccac gtagtgcggc tccttgtcaa
      781 ggcaggtgaa gaactgatgc gtgccgaggc ggtccggacg ctggttgaac gtggtggaga
      841 tgccatccgt gcccgtgcag aggccgttgt tgaccaccac ggaatagatg ccaccggagg
      901 tgacattgat cgtgcgggtg tcctgtccgt tgctccattg gtaggttgca ccggggttgc
      961 cggcgtcgag agtgagcaca tcaccttcgc acagcacggt atccgggccc aggtccacca
     1021 ccggcggatc ggcgaaggtg acctccgcat cgaagtggcc ggaacagttc atcgcattgg
     1081 tcacggtgac actgtacgtc ccggaggaga ccacgtcgat cgttggcgtg gtggcgccgg
     1141 tgttccagag ccaggtgcaa ccggggttgc ccgcgtccag ggtgatggtt ccgcccgcgc
     1201 acgcgatggt gtcctgcaag gggtccgttg gggaggggtc gaaggtcagc gagatggcat
     1261 cctgccccgc gcacgcacca ttggaagcgg agacgctgta cgtaccgctc gtggtggcct
     1321 ggatggtctg tgtggtggcc cccgtgttcc aggtataatt caagccggac gtgcccgcgt
     1381 ccagcacgag tacctggcca gagcacagcg tagtgtccgg cccaaggtcg atgttgacca
     1441 ccggcaggat cgcgacgatc gcgacgtagg tggcggcgca ctgttcaggg gtgatgatgg
     1501 tcacgctgta ggtgccactg ctgtccggaa cgatggactg ggtggtagcc ccgttgttcc
     1561 actggtagct ggcgcccggg ttgcccgcgt tcaatgacac cggcgtgccg gcacacacgg
     1621 tggcatcagc cagttcatcc gtgggcaggg catggaatgc gatgttcacc tcgtcggccg
     1681 cggtacaatt gaacggtgtg gtcacgctca ccgaataagt gccgtcctgt gtcacggtga
     1741 tctgctggtc gttggccccc gtgctccaac tgaatgcatt
//
LOCUS       DAOQOC010000713         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48313_length_1778_cov_2.953569, whole genome shotgun sequence.
ACCESSION   DAOQOC010000713 DAOQOC010000000
VERSION     DAOQOC010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48313_length_1778_cov_2.953569"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            442..1113
                     /locus_tag="PL106_11040"
     CDS             442..1113
                     /locus_tag="PL106_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33645.1"
                     /translation="MGNRAADDSDQAWAIARALSSPLGVGNALFRLGTTEECEVDTVP
                     YGCWCRSLFDRIGLFDVELLRNQDDEFNGRIIRHGGRIVMLPGVRIRYTARKDLSRLW
                     RMYREYGLFKPLVNRKLGAPATARQFVPPLFVLALVGLPVLALFAPVFAVPWCAMLVL
                     YLSVVALVSARIAFSGGRPRALPWMMAAFACTHLSYGIGYLQGIWRFLIIRRAMPPGD
                     LATNR"
     gene            1110..>1778
                     /locus_tag="PL106_11045"
     CDS             1110..>1778
                     /locus_tag="PL106_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA33646.1"
                     /translation="MSTAMRLLLLSGASSTHTIRWANAFVDRGIGTHLVTQHDPVDPP
                     DPRVHVHRLPHLGGAGYAFNGPRLRALVQEYRIDVINAHYATGYGLLARWRGRVPCVL
                     NVWGSDVYDFPEKSALHRAFLRTNLRAADRIISTSHAMAARTRAICPGLGSVTVVPFG
                     VDTTCFAPASTGRSADAPRVVGTVKVLDEKYGIDTLIDAFARVVRHADRSVRLRIVGD
                     GPQRP"
BASE COUNT          268 a          594 c          579 g          337 t
ORIGIN      
        1 atccacgggc gaacgcggcc gaagtgctgg cccacctgcg cggcgtggcg cttcatcgtc
       61 caacgaacgg agcggtcgcc gcatgagcag gctggtgagc atcgtgatgc cctgccgcaa
      121 cgaggcggat catctggcgc atgccgtgga cgacgcgttc gcccaggaac gcgacggctt
      181 ggaactggag gtgctcctgg ccgtgggacc cagtaccgat ggcaccgagc tgatcgcccg
      241 gaccctcaag gaccgctacg ctgacctgca cgtattgccc aacccgcaag gcgtggtgcc
      301 ttatggactg aatgcggcca tccgcgcggc ccacggggat gtcatcgtgc gcatggatgt
      361 ccattcgcgg tatccggcca actatgtggc cgccctggtg cgagcgctcg accgcaccgg
      421 tgctgacaac gtgggtggtg tgtgggaaac cgtgccgccg atgactcgga ccaggcctgg
      481 gccatcgccc gcgccctgtc gagccccctg ggggtgggca acgcgctgtt ccggttgggc
      541 actaccgagg aatgtgaagt ggatacggtg ccctacggct gctggtgccg ctcactcttc
      601 gatcgtatcg ggctgttcga tgtggaactg ctgcgcaacc aggatgatga gttcaacggg
      661 cggatcatcc gccatggtgg gcgcatcgtt atgctgccgg gggtgcgcat ccggtatacc
      721 gcccggaagg acctgtcccg gctctggcgc atgtatcgcg agtacggcct cttcaaaccg
      781 ctggtcaatc gcaagctggg tgcaccggcc accgcacgcc agttcgtgcc cccccttttc
      841 gtactcgcgc tggtgggcct gcccgtgttg gccctgttcg cgccggtgtt cgccgtgccc
      901 tggtgcgcca tgctcgtact ctacctgtcc gtggtcgccc tcgtttccgc gcgcatcgct
      961 ttcagtggcg ggcgtccgcg cgctctgccc tggatgatgg ccgccttcgc ctgcacacac
     1021 ctgtcctacg ggatcggtta cttgcagggg atctggcgct tcctgatcat ccgacgtgcc
     1081 atgccaccgg gcgatctggc caccaaccga tgagcaccgc catgcgattg ttgctgctct
     1141 ccggtgcgag ttccacacat accatccgct gggcgaacgc gttcgtggac cgggggatcg
     1201 gaacgcatct ggtcacgcag cacgacccgg tggatccgcc cgatccccgc gtccacgtcc
     1261 accgtttgcc gcacctgggc ggtgccggat acgcgttcaa tggaccacgc ctgcgggcat
     1321 tggtacagga ataccgtatc gacgtgatca atgcgcacta cgccacgggg tacggcctcc
     1381 tcgcgcgctg gcgcgggcgg gtgccctgtg tactgaacgt gtggggcagc gatgtgtacg
     1441 acttcccgga gaagagtgcg ctgcaccgtg ccttcctgcg taccaacctg cgcgcagcgg
     1501 accggatcat ctccaccagc cacgccatgg ccgcccgcac gcgtgcgatc tgtccgggcc
     1561 tcggatccgt taccgtggtc ccgttcggtg tggatacgac ctgcttcgca ccggcatcaa
     1621 caggtcggtc ggctgatgcc ccacgcgttg tgggaaccgt taaagtgctc gatgagaagt
     1681 acgggatcga taccctgatc gacgccttcg cccgggtcgt gcggcatgcc gatcgatcgg
     1741 tacgcctgcg catcgtgggc gacgggccgc aacgcccc
//
LOCUS       DAOQOC010000714         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48359_length_1777_cov_3.374564, whole genome shotgun sequence.
ACCESSION   DAOQOC010000714 DAOQOC010000000
VERSION     DAOQOC010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48359_length_1777_cov_3.374564"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PL106_11050"
     CDS             complement(<1..1650)
                     /locus_tag="PL106_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33647.1"
                     /translation="MTIKRSLLLALFGTSLLLNAQDEKVRWKTYTGTEKNQDGGFWYH
                     KFVKGDAGSIVGLRVLTKSPVIGGMNQVSIDRTLVAFQLDGMKEVKADKTKLLWGEGP
                     VSVETIEHFNKQFRVIASKADPETGKLLVIQQVLGPRSLTGKGAELLAEIPFDRLGKT
                     NAYFKPNITVGFTTLVSVDSTKMLLQLTPESTVHSAGCPIYAHLFDKEMKSLWWNKLS
                     PDPGAKSSRIMSTKVDPTGAVWYLIKNVSDPDPKTKGEAGYNWSIYRLDSAGQTTALL
                     DLPGSEFVQDARMDMRLNGTITVAGVYSDAEMDRNVSVGVFQCTVNGAGKEPKFDGFK
                     LYPFAKRIEGKEEKPQVNMIMDRLLLKKDGGTFVIARKSGEETHYVSDLSGKKTPKTE
                     QVDGAIHIFELAADGSQKWYKQLEREMGSANNALGQILPMVYENNLLLLLNDAEGNIE
                     KRKQKLPIAPVDDFKDAILVEYKSDGTDKSKVVLTEPRRLGIVAGSAWQPTAGLVVLL
                     ASLMLLAVAILLIVVTQRFCEVLEVEEPPPVSHYPPPRPAVG"
BASE COUNT          292 a          560 c          512 g          413 t
ORIGIN      
        1 cccgactgcg ggacgtggcg gcggatagtg cgagaccggc ggaggttcct cgacttccag
       61 cacttcgcag aaacgctgtg tcaccacgat cagcagaatc gcaactgcca gcagcatgag
      121 gctggcgagc agcaccacca gaccggccgt gggttgccat gcgctgcccg ccacaatgcc
      181 cagtcggcgc ggttcggtca ggaccacttt gctcttgtcc gtgccgtcgc tcttgtactc
      241 caccagaatg gcatccttga agtcgtccac aggggcgatg ggcagcttct gcttgcgttt
      301 ctcgatgttg ccctccgcat cgttcagcag caagagcagg ttgttctcgt acaccatggg
      361 caggatctgg cccagcgcgt tgttcgcact gcccatttcg cgctccagtt gtttgtacca
      421 cttctggctc ccgtccgccg ccagttcgaa aatatggatg gccccatcca cctgttcggt
      481 cttgggggtc ttctttccgc tgaggtcgct cacatagtgc gtttcctcac cgctcttgcg
      541 ggcgatcacg aaggtgccgc cgtccttctt caagagcaac cggtccatga tcatgttcac
      601 ctggggtttt tcctccttgc cctcgatccg cttcgcgaac gggtacagct tgaacccatc
      661 gaacttgggc tctttgccag ctccgttcac cgtgcattgg aaaacgccaa cggaaacatt
      721 gcggtccatc tcggcatccg aatacacccc cgccacggta atggtgccgt tgagccgcat
      781 gtccatccgc gcatcctgca cgaattcgct accgggcagg tccagcagag ccgtggtctg
      841 gccggcactg tcgagccggt agatggacca gttgtacccc gcttcgccct tggtcttggg
      901 atccgggtcg ctcacgttct tgatcaggta ccataccgcg ccggtcggat ccaccttcgt
      961 gctcatgatc ctggaactct tggcgcctgg atcagggctc agcttgttcc accagagcga
     1021 cttcatctcc ttgtcgaaca ggtgggcata gatcggacaa cccgcgctgt gtaccgtgct
     1081 ctccggggtc agttggagca gcattttggt gctatccacg ctcaccaggg tggtgaagcc
     1141 taccgtgatg ttgggcttga agtacgcatt ggtcttgccc agcctgtcga acgggatctc
     1201 ggcgagcagt tccgcgccct tgcccgtgag gctgcgaggg cccagaacct gttggatcac
     1261 caggagcttg cctgtttccg gatcggcctt gctggcgatc acgcggaact gcttgttgaa
     1321 gtgctcgatg gtttccacgc ttaccgggcc ttccccccac agcagcttgg tcttgtcggc
     1381 cttcacctcc ttcatgccgt ccaattggaa ggccaccagg gtgcggtcga tggacacctg
     1441 gttcatcccg ccgatcacgg gactcttggt gagcacccgc agacccacga tgctgccggc
     1501 gtcgcccttc acgaatttgt ggtaccagaa accgccatcc tggttcttct ccgtgcccgt
     1561 gtatgtcttc cagcgcacct tttcatcctg ggcgttcaac agcagcgagg taccgaaaag
     1621 tgcgagaagc agggagcgtt tgatggtcat tgtcgatcgg atgcggttgt gtgaagcggg
     1681 tgcaaagtag tcctctccga atggatgggg caccgctgtg gtccggatcg gcagtttccg
     1741 tgccagggcc tgccccgggg ttcattcccg gcttgcg
//
LOCUS       DAOQOC010000715         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48473_length_1775_cov_3.061628, whole genome shotgun sequence.
ACCESSION   DAOQOC010000715 DAOQOC010000000
VERSION     DAOQOC010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48473_length_1775_cov_3.061628"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PL106_11055"
     CDS             complement(<1..294)
                     /locus_tag="PL106_11055"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNA33648.1"
                     /translation="MSFTTASSAERVPFGRNALLRVQLVLFLIAWAATFIGTTDRTNW
                     FTENTLTALLIGGLCISYRKFRFSDLSYTLFFIYILLHIYGAMYTYAENPLGYW"
     gene            complement(308..1084)
                     /locus_tag="PL106_11060"
     CDS             complement(308..1084)
                     /locus_tag="PL106_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA33649.1"
                     /translation="MKRLIPLFVASLVMTTVQGQVSMGHRAGLSIASQRSEKEDFLKG
                     RGIGGRPMDDPHALMGVSSALVVEAMIKPYIGVQLELGITQKGRQISEKNFDQGRNYY
                     RLRLNYGEGLLLLKGTVGKGSVKGNLLGGISFGRGLSAVERGKFSVDSLSGEVVDRRD
                     SASIKELEDRFIDFDDVTKGFLAPTELCWVVGAGVSFDIGRSRIFLEGRLNQGLSAVY
                     NGKLNEPDVFNRAILVQAGYLVRLNDRKEKKPVSPDTPSW"
     gene            complement(1130..>1775)
                     /locus_tag="PL106_11065"
     CDS             complement(1130..>1775)
                     /locus_tag="PL106_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA33650.1"
                     /translation="NWTLLCAALIPGLLLGQARQGPKLGLAVASQTAGGFGWAGLPKF
                     GPIVGWSWDLPLTGQAHLLVEPLYVSKGYWTRNSVWNINTYGTFPYLELQALLKLDLS
                     KGKEDGMFLTGGPIYGYALRGRVKEFTAGKKTHDYKAQPYPASQFSVAVGLGTATKRW
                     AYELRAQTSMSPWTPTIRAQNLVISLNLTYRLLSYEERQAKREKKELNEEEQED"
BASE COUNT          322 a          570 c          516 g          367 t
ORIGIN      
        1 ccagtacccg agcgggttct ccgcgtacgt gtacatggcc ccgtagatgt gcagcaggat
       61 gtatatgaaa aacaaggtat aactcaggtc gctgaacctg aacttgcggt acgagatgca
      121 caggccgccg atcaagaggg ccgtgagcgt gttctcggtg aaccagttcg tccggtcggt
      181 ggtgccgatg aaggtggccg cccacgcgat caggaacaac accagttgga cacgcagcag
      241 cgcgttgcgc ccgaacggga cgcgctccgc ggaggaagcg gtggtgaagc tcatgcgcgc
      301 ctggggatca ccacgatggt gtatcgggcg aaacgggctt cttctccttc cggtcgttca
      361 agcgcaccag gtagcctgcc tgcacgagga tggcccggtt gaacacatcg ggctcgttga
      421 gcttcccgtt gtaaacggcg gagagcccct ggttgagccg accctccagg aaaatgcgcg
      481 atcggccgat atcgaagctg acaccggcgc cgaccaccca gcagagttcc gtgggcgcaa
      541 ggaagccctt ggtcacgtcg tcgaagtcga tgaagcggtc ctccaattcc ttgatgctcg
      601 ccgaatcgcg ccggtccacc acttcgcccg agagggagtc cacgctgaac ttgccgcgct
      661 ccacggcgga gaggcccctg ccgaaactga taccacccaa caggttgcct ttcacgctcc
      721 ccttgcccac ggtacctttc agcagcagca gtccttcgcc ataattcagc ctcaatcggt
      781 agtaattgcg cccctggtcg aagttcttct cggagatctg gcgacccttc tgggtgatgc
      841 ccaactccag ttgcacgccg atgtacggct tgatcatcgc ctcgaccacc agcgccgaac
      901 tgacgcccat gagcgcatgc gggtcgtcca tgggacggcc cccgatgccc cggcccttga
      961 ggaaatcctc cttttcggac cgctggctgg cgatgctcag gcctgcacgg tggcccatgg
     1021 aaacttgtcc ttgcaccgtg gtcatcacca aggaggccac gaacagcggg atgagtcgtt
     1081 tcatgttggt tgggatcgaa ccggtcgaag atagggtttc accggtattt taatcttctt
     1141 gttcctcctc gttcagctcc ttcttttcgc gcttcgcctg gcgctcctca taactcagca
     1201 gcctgtatgt caggttgagg ctgatgacca ggttctgcgc gcgtatcgtc ggggtccatg
     1261 ggctcatgct cgtctgtgcc cgtaactcgt acgcccatcg cttggttgcc gtgcccaggc
     1321 ccaccgccac actgaactgg ctggccgggt acggttgcgc cttgtaatca tgggtcttct
     1381 ttcccgctgt gaattccttc acccgccccc gcaacgcgta gccgtagatc ggccctcccg
     1441 taaggaacat cccgtcctcc ttccccttgc tcaggtcaag tttcaacagg gcctgcaact
     1501 ccagatacgg gaaggtgccg taggtgttga tattccacac ggaattccgg gtccagtacc
     1561 ctttgctcac gtacaggggc tccaccagca ggtgcgcctg cccggtgagt ggcaggtccc
     1621 agctccaacc tacgatgggt ccgaacttgg gcaggcccgc ccatccgaaa ccaccggcgg
     1681 tctggctggc cacggccagg cccagtttag gtccttgtcg cgcctgtccc agtagcaggc
     1741 ccggtatcag tgcggcgcag aggagcgtcc agttc
//
LOCUS       DAOQOC010000716         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48694_length_1771_cov_3.302448, whole genome shotgun sequence.
ACCESSION   DAOQOC010000716 DAOQOC010000000
VERSION     DAOQOC010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48694_length_1771_cov_3.302448"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..>1771
                     /locus_tag="PL106_11070"
     CDS             137..>1771
                     /locus_tag="PL106_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNA33651.1"
                     /translation="MKGSLSVVLLASFTLAVRAQQHYGNEWIDHDQRYWAFWVGTYPD
                     GSAFEGIWRIDSVTLADAGFPIGSTDARAIQVFGRERQVAIWFPGDSDGVFNAADHIE
                     FYVPPNDAWLDSALWDDPAHINNPYLSSIGDSIQYFVTIGDPQQSLRAIDQDPGDWAA
                     LPNAEPWYWADVFELPWPGQFTYKRGRRDYWGSTTSWMGDGEGFSFFDMVNDGDDDPN
                     IVNLITPFPWSDPSAPPMKLELAVMGANAAGNLQYIDHKLMVTAGVAPNQASVDSSWI
                     AEKTIKLGLEIPAGGYVSPITPVELNIVHDLHQTVSPQLDQYYPDRQALAYVKATYPH
                     AIGAGTFPAQFYRMHFDNDGQDIRLDFTMPGEPVVWVFGDQVRRVNVEDANGGWNCRL
                     PFTQGSATTDAVLTVKAAARPVTGFYPVNGTGYFTDHLAVSADSALIIVTHPALMNGT
                     LQYAQYRETNTHNRFSTVVANVLELYQQYGGGILRHPVAIRRFVKQLADNATYGPQGL
                     FLVGKATQAAKLANGDLSGYRSISPQDSVVRKMCLVP"
BASE COUNT          344 a          544 c          556 g          327 t
ORIGIN      
        1 aacccaacgt gggtgtcggc gtgaagatca agagcgtggt ggtggactac gcgctcacca
       61 acatcggcag caccggggtg gccttgtact ccaacgtctt ctcgctgcgc ttcgacctgt
      121 tcaagcacaa gggcacatga agggatcatt gtcggtggtg ctgctcgcgt ccttcacgct
      181 cgccgtgcgg gcgcaacagc actacggcaa cgagtggatc gatcatgatc agcggtactg
      241 ggcgttctgg gtgggcacat atcccgatgg ttccgctttc gaggggatct ggcggatcga
      301 cagcgtgacc cttgccgatg cgggcttccc gatcggttcc acggatgcga gggccatcca
      361 ggtcttcggc cgggaacgcc aggtggccat ctggttcccg ggagattcgg acggggtgtt
      421 caacgcggcg gatcacatcg agttctatgt gccgcccaac gacgcgtggc tggacagtgc
      481 cctgtgggac gacccggcgc acatcaacaa cccgtacctg tcctccatcg gcgacagcat
      541 acagtatttc gtcaccatcg gcgatccgca gcaaagcctg cgagcgatcg atcaggaccc
      601 cggcgattgg gcggcacttc ccaatgccga accttggtac tgggccgatg ttttcgaact
      661 gccgtggccc ggacaattca cctacaaacg ggggcgccgt gactactggg gctccactac
      721 gagttggatg ggcgatggcg aaggcttctc cttttttgac atggtgaacg acggggatga
      781 tgatccgaac atcgtcaact tgatcacgcc cttcccatgg tccgatccct cggcaccgcc
      841 catgaaactg gagttggccg taatgggtgc caacgcggcc gggaatctcc agtacatcga
      901 ccacaaactg atggtcaccg cgggggtggc cccgaaccag gcctccgtgg acagttcgtg
      961 gatcgctgag aagaccatca agctgggcct tgagataccg gcaggcggtt acgtcagccc
     1021 gatcacacca gtggagttga acatcgtgca tgacctgcat cagacggtga gtcctcagct
     1081 cgatcagtac tatcccgacc ggcaggccct ggcatacgtc aaagccacct atccgcacgc
     1141 catcggggcc ggcacgttcc ccgcgcagtt ctaccgcatg cacttcgaca acgatgggca
     1201 ggacatccgc ctggatttca ccatgccggg cgaaccggtg gtctgggtgt tcggggatca
     1261 ggtgcgtcgg gtgaacgtgg aggacgcgaa cggggggtgg aattgccggc ttccgttcac
     1321 acaggggagc gcgaccaccg atgcggtcct cacggtgaag gccgccgcgc gcccggtcac
     1381 gggattttac ccggtgaacg gtaccggcta cttcaccgat cacctggccg tgagcgcgga
     1441 cagtgccttg atcatcgtga cgcatcccgc gctgatgaac ggaacgttgc agtacgccca
     1501 ataccgggag accaacacgc acaaccgttt cagcacggtg gtggccaatg tgctcgaatt
     1561 gtaccagcag tacgggggag gcatcctgcg gcatccggtg gccatccgcc ggttcgtgaa
     1621 gcaactggcc gacaacgcca cgtacggacc ccaggggctc ttcctggtgg gcaaagccac
     1681 acaggccgcc aagctcgcca atggcgacct gtccggttac cgcagcatca gcccgcagga
     1741 ttcggtggtg cggaagatgt gcctggtccc g
//
LOCUS       DAOQOC010000717         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_48695_length_1771_cov_3.300699, whole genome shotgun sequence.
ACCESSION   DAOQOC010000717 DAOQOC010000000
VERSION     DAOQOC010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48695_length_1771_cov_3.300699"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1571)
                     /locus_tag="PL106_11075"
     CDS             complement(183..1571)
                     /locus_tag="PL106_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNA33652.1"
                     /translation="MRSTLLLLVLCLANASYTQSIQFQQAVTGFSSPVDIAHCGDERL
                     FVVERPGRIRIVRNGAINPVPFLDITGIVNDGGGEQGLLGLAFHPQYAANGWFYVFYV
                     GGTGNGFTAVARYNVSGNPDVADPNSALVMWSLAQPYANHNGGDIDFGPDGKLYFSTG
                     DGGDGGDPGNRAQDLGLHFGKILRVTPTSGGYSIPANNPFVGQQGALPEIWAYGLRNP
                     WRFGFDRQTGDLWIGDVGQGAWEEVDKWPVGNNSGPNFGWRCYEGGAAYNTAGCQPQS
                     SYVGPVAVGAHGSSQGPNGGEWCSVIGGRVYRGNKYPALVGKYLYTDYCHGRIHWIDA
                     ADPSSQGTFTGSGIYGFSAIAEDRCGELWAVNVNNGRLYRIVDTDVCPEDVNRDGIVK
                     YTGTNNDRDPVLIAVGATSPNNPAPCACCLADVTGDGVVKYTGNGNDRDPIILGVGST
                     SPNNTVACPDGQ"
BASE COUNT          322 a          558 c          545 g          346 t
ORIGIN      
        1 ccgtgcatgc gggagatggc gtacgtatga gtgctgaact ggcctccggg cgattcgcct
       61 ccggccgttg ggcactgatc gatggtgcgg atcagcagtc aggtccgttg ttcctgaccc
      121 tgatcgacaa agagaccggc gaacgctggt atgccggcgt gctggagccg tgaccggacc
      181 aattactgac cgtcagggca ggccacggta ttgttgggtg acgtacttcc cacgcccagg
      241 atgatcgggt cgcgatcgtt cccgttgccc gtgtacttga ccacgccatc gcccgttaca
      301 tcggccaggc agcaagcgca aggggccggg ttgttcgggc tggtggcccc caccgcgatg
      361 agcaccgggt cgcggtcgtt gttggttcca gtatatttta cgataccgtc ccggttcaca
      421 tcctccgggc agacgtccgt gtcgacgatc cggtacagcc ggccattgtt cacgttgacg
      481 gcccaaagct ccccacatcg gtcctcggcg atggccgaaa aaccgtagat gccgcttcct
      541 gtgaacgtcc cctgggacga gggatcagcg gcatcgatcc aatggatcct tccatggcag
      601 tagtccgtgt acaggtactt gccgaccagt gcggggtatt tgttcccgcg atagacccgg
      661 cccccgatca ccgagcacca ttcgcccccg ttcgggccct ggctgctccc gtgcgcaccc
      721 acggcaacgg gacccacata ggatgactgg ggctgacagc ccgcggtgtt gtacgccgca
      781 ccgccctcat aacagcgcca accgaaattg ggcccgctat tgttccctac gggccacttg
      841 tccacttctt cccatgcgcc ttgtcctaca tcaccgatcc acagatcccc tgtctggcga
      901 tcgaagccga accgccaggg attgcggagc ccgtatgccc agatctcggg cagtgcgcct
      961 tgctggccta cgaacgggtt gttcgccggg atcgagtatc cgccggatgt gggtgttacg
     1021 cgcaggatct tgccgaaatg gaggcccagg tcctgtgccc ggtttccagg atcgcctccg
     1081 tcgccgccat caccggttga aaagtacaat ttaccatccg ggccgaaatc aatatcacca
     1141 ccgttgtggt tggcgtacgg ttgggcgagc gaccacatca ccagcgcgct gttcggatcg
     1201 gccacatcgg ggttgccgct cacgttgtag cgggccacgg ccgtgaagcc attgccggtg
     1261 ccgcccacgt agaagacata gaaccatccg ttcgccgcgt actggggatg gaatgccaat
     1321 ccgagcaggc cctgctcccc gccgccgtcg ttcacgatgc ctgtgatgtc gaggaagggg
     1381 acggggttga tcgcaccgtt ccgcacgatg cggatgcgcc cagggcgttc aacgacgaac
     1441 aggcgctcat caccgcaatg ggcgatgtcc accggcgatg agaagccggt gaccgcttgt
     1501 tggaattgga tggactgggt gtacgaggcg ttcgccaggc acagcacgag gaggagcagt
     1561 gtggagcgca tgcagcaagt ttgtatcaat gggcatgcgg gtggggacat tactcggcat
     1621 atcccggatg ttccagggat caacgcgccg atccgctcga cgggaacgat cgcgttcctc
     1681 ccgaccttcg taacctcgcg catcgttgat caccatggag accccggcga tccgcatcgg
     1741 aacacacgga ctgtccgtgg actacctcgt g
//
LOCUS       DAOQOC010000718         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_49091_length_1765_cov_2.341520, whole genome shotgun sequence.
ACCESSION   DAOQOC010000718 DAOQOC010000000
VERSION     DAOQOC010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49091_length_1765_cov_2.341520"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PL106_11080"
     CDS             <1..1102
                     /locus_tag="PL106_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33653.1"
                     /translation="GGLSPDNQLKLAAMMYEGAGSSDHYPGPLTVGGDATTTSGMMAV
                     YDHVWSVTRAEIDLHLAYFNCLNDPNCDLNAEFPDGYTIPPSILDWPAINTAVGYDHF
                     QAPFIDFNSNFDQYEPQAGDAPCILGDQALYFVFNDKGGAHLLSGAQPIGIEVQAMAF
                     AFTGDDPALEQTVFVHYHIINRGTQTLTNTYLGLFNDFDLGCGDDDLVGSDPARNLVY
                     VINGDDDDGDCSGVIGYGTQPPAFGMVVLKGPYLDPNAVDDPVVSTLPAWNGGGFDDQ
                     TIDNERFGITSSMHIYRDGDQCCTAPTSGSSNQFNNYLRGIWKDGVPFSYGGTGHSTD
                     PDAVDCAFSFPGNSDPLGVGTAGPPGRTRHRP"
     gene            1102..1590
                     /locus_tag="PL106_11085"
     CDS             1102..1590
                     /locus_tag="PL106_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33654.1"
                     /translation="MDRRMLTSSGPFTLVPGMHQDLVVAYVFARASSGGALASLDALR
                     TRVDSVTTFAHTLPLFDIPQDQFQGGCDLGLPDAVVHVNDPEIFEPFHVPASNEVTLM
                     APNSVSTGTIRLLDATGRVVVSQQLVPGRNAIDIAGLANGVYICDVLAGREHFTTRLV
                     KQ"
BASE COUNT          329 a          544 c          510 g          382 t
ORIGIN      
        1 gggcgggctg tcgccggaca accaattgaa gttggccgcc atgatgtatg aaggcgcggg
       61 ttcatccgat cactacccgg gtcctttgac cgtgggtggc gacgcaacca ccacctcggg
      121 catgatggcc gtgtacgatc atgtctggtc cgtgacacgg gcggagatcg atctgcacct
      181 cgcctacttc aattgcctca acgatcccaa ctgtgatctc aatgctgaat tcccggatgg
      241 ctataccatt ccaccgagca tcctggattg gcccgccatc aatacggcag ttggttacga
      301 tcattttcag gctcccttca tcgacttcaa ttccaatttc gaccaatatg agcctcaggc
      361 gggcgatgcc ccctgcatcc ttggcgatca ggctttgtac ttcgtgttca acgataaagg
      421 aggagcgcat ctcctcagtg gtgcccagcc gatcggcatc gaggtgcagg ccatggcttt
      481 cgctttcacg ggagatgatc ctgccttgga acagacggtc ttcgtccatt accacatcat
      541 aaaccgcggc acgcaaacgc ttacgaacac ctacctcggt ctgttcaatg acttcgacct
      601 gggttgcggc gatgatgacc tggtgggctc cgatcccgcc cgcaatttgg tctatgtgat
      661 caatggcgac gacgatgacg gcgactgctc aggcgtgatc gggtacggca ctcaaccccc
      721 cgccttcggt atggtcgtcc tgaagggccc ttaccttgat cccaatgccg tggatgaccc
      781 ggtggtgtcc acgcttccgg catggaacgg tggtggattc gacgatcaaa cgatcgacaa
      841 tgaacggttc ggtatcacaa gctccatgca catctaccgc gatggcgacc agtgctgcac
      901 agcgcctacc tcaggttcga gcaaccagtt caacaactac cttaggggga tctggaagga
      961 tggcgtgccg ttctcgtacg gtggaaccgg tcatagcacg gaccctgatg ccgtggattg
     1021 tgcgttcagc tttccgggga acagcgaccc cctgggcgtt ggaacggcgg gcccgcctgg
     1081 tcggacacgc catcgtcctt gatggaccgc aggatgctca ccagttccgg gcccttcacc
     1141 ttggtgccgg gcatgcacca ggacctggtc gtggcctacg tgttcgcgcg cgcttcctcg
     1201 ggcggcgcac ttgccagctt ggacgccttg cgtacacgcg tggactcggt gaccaccttc
     1261 gcacacaccc tgccgttgtt cgacatccct caggatcagt tccaaggcgg ttgcgacctc
     1321 gggctgcccg atgcggtggt ccatgtgaat gatcccgaaa tctttgaacc attccatgtt
     1381 ccggcatcga acgaggtgac cctgatggcc ccgaactcag tgagcacggg caccatccgt
     1441 ttgctggatg cgaccggccg ggtggtggtc agtcaacaac tggtgcccgg gcggaacgcg
     1501 atcgatattg cagggctcgc gaacggtgtg tacatctgcg atgtgctcgc tggccgcgag
     1561 catttcacta cgcgcttggt gaaacagtga tccctgccgt gccgggagcg gaccggatca
     1621 tgcgccgctc catgtgttca atagggcagg agggcagccc tcgcccgtgc gcctgctctt
     1681 tggatcagcc agatccttcc attgatcggg gcgaacggtc atcgcgcatc cgcccctatg
     1741 tccgcccctg ctgccagaag tcata
//
LOCUS       DAOQOC010000719         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_49127_length_1764_cov_3.372147, whole genome shotgun sequence.
ACCESSION   DAOQOC010000719 DAOQOC010000000
VERSION     DAOQOC010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49127_length_1764_cov_3.372147"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PL106_11090"
     CDS             complement(<1..1414)
                     /locus_tag="PL106_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33655.1"
                     /translation="MMQGLLSATAATILLSSTAVGQERATCLYAEPFSGPDMPAGWAV
                     TPAQVALLDQNGDTTGGFTGPWIIGNAEQANAAGYFPVPDEPHGNTFAMANDDAPPCD
                     CAMSDVSLVSPVYDLSTVPAPAVSYRVYHDGLPTNGRAWLDASTDGTVWTTLEEIPAV
                     HNAWQHRLVDLGAFAPGSVQLRFRYDDNGNWSSGMAVDDMCIFTRVADDIALVDAWLG
                     DRTVSPFITDVRSLGYSRIPVEQQEPLLLSARVRNNGLNVATDITVETTITPENGTPV
                     TVGPVVVNALAPLHDTLVTWNTGFVADVAGHVNISLSIFAANADEEPSDNDASLTYEV
                     TAPDVGNHAMALDNDQPSVALGDDGFSVGCRYELNEGQATIHGVSTRFGPGTALGARV
                     RVVLMNGELNPLHVSSMHTITEEDLQLSFNGGTVYLPLDSVLDVDGPQDVIALVHSPP
                     DSGAVRIMAGGNSPVGANWSI"
     gene            complement(1414..>1764)
                     /locus_tag="PL106_11095"
     CDS             complement(1414..>1764)
                     /locus_tag="PL106_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HNA33656.1"
                     /translation="FPVAETLMVEPTESEAKAELDRFIEAMIGIRQEIAALEQGKADK
                     LDNVLKNAPHTSEEVCGNTWDHAYPREQAAFPANVNREWKYWPVVSRVDNAYGDRNLI
                     CVCPPIEEYMTAEA"
BASE COUNT          292 a          541 c          588 g          343 t
ORIGIN      
        1 ggatcgacca gttggcgccg accggtgagt tgccgccagc catgatgcgg acagcaccgc
       61 tgtccggcgg gctgtgtacc agtgcgatca cgtcttgtgg cccgtccaca tccagtacgc
      121 tgtccagcgg caggtacacc gtgcctccgt tgaagctgag ttgaaggtcc tcctcggtaa
      181 tggtgtgcat ggaggaaacg tgcagcgggt tgagttcgcc gttcatgagc accacgcgca
      241 cacgcgcccc gagggccgtt cccggtccga atcgcgtgct cacaccgtgg atggtcgctt
      301 gaccttcgtt caactcgtaa cggcagccca ccgagaagcc atcgtcaccc agtgccacgg
      361 aaggctggtc gttgtccagg gccatggcgt ggttgcccac atcgggtgcg gtcacctcgt
      421 aggtcagcga agcgtcgttg tccgatggtt cctcatcggc gttcgcggcg aagatgctca
      481 agctgatgtt cacgtgcccg gcaacgtcgg ccacgaaacc ggtgttccac gtgaccaacg
      541 tgtcgtgcag cggtgcgagc gcgttcacaa ctaccgggcc aacagtaacg ggtgtgccgt
      601 tctccggggt gatcgtcgtt tccacggtga tgtcggtggc cacgttcagc ccgttgttcc
      661 gcacccgggc ggagagcagg agcggctcct gctgttccac ggggatacgg ctgtagccca
      721 atgaacggac atcggtgatg aaggggctca cggtacgatc gccgagccag gcatccacca
      781 gcgcgatgtc atccgcaacg cgggtgaaga tgcacatgtc gtccacggcc atgcccgaag
      841 accagttgcc attgtcgtcg taccggaagc ggagttgcac ggatcccggt gcgaaggcgc
      901 cgaggtcgac caggcggtgc tgccaggcgt tatgcacggc ggggatctcc tccagggtgg
      961 tccataccgt tccgtcggta ctcgcatcaa gccaggcacg tccattggtc ggcaggccgt
     1021 cgtggtacac gcggtagctc acggcggggg cgggcacggt gctcaggtca tatacgggcg
     1081 atacgagcga cacgtcgctc atggcgcaat cgcacggtgg cgcgtcgtcg ttggccatgg
     1141 cgaaggtgtt cccgtgcggc tcatcgggta cgggaaaata cccggcagca ttggcctgct
     1201 cggcgttccc gatgatccac ggtccggtga agccgccggt ggtgtcgccg ttctggtcga
     1261 gcagggccac ttgtgcgggc gtaacggccc agcctgcggg catgtcgggg ccgctgaacg
     1321 gttcggcgta caggcaggtc gcacgctcct gcccaacggc ggtggaagag agaagaatgg
     1381 tcgcggcggt cgcggaaagc aggccctgca tcatcacgcc tccgcggtca tgtactcctc
     1441 gatgggcggg cagacacaga tcaggttgcg gtcgccgtag gcattgtcca cgcggctcac
     1501 cacaggccag tatttccact cccggttcac attcgccggg aaggcggcct gctcacgcgg
     1561 ataggcatgg tcccacgtgt tcccgcatac ttcttcgctg gtatgcgggg cgttcttcag
     1621 cacattgtcc agtttgtcgg ccttgccttg ttcgagtgcg gcgatctcct ggcggatgcc
     1681 gatcatcgcc tcgatgaaac ggtccagttc ggccttggct tcgctctcgg tgggttccac
     1741 catgagcgtt tcggccacgg ggaa
//
LOCUS       DAOQOC010000720         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_49176_length_1763_cov_3.635246, whole genome shotgun sequence.
ACCESSION   DAOQOC010000720 DAOQOC010000000
VERSION     DAOQOC010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49176_length_1763_cov_3.635246"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PL106_11100"
     CDS             <1..1100
                     /locus_tag="PL106_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33657.1"
                     /translation="LRETMIPTIHMIPFKHILFGAVVATAAPALAQQPRIVVQHAGSV
                     QVFSDLGEAITAAQNNAELYLSGGSFVLPGGLALSKTLHFIGAGVHTDSTAASGPTIL
                     STGTSANFRLNSSASGSSFSYITFNVPGNGICFGLGTSELDQTVESVEFHRCVFWQTV
                     SLGVVAPCGSTSSFTECIFHSTLYGCNATTQVTRCIFDYQAGTGAEISAFQGGGLTML
                     NCVCLGTRIGDSGASVVMNSVFTRTSAPFWQSSGMTMTNNLLVSPDLTSNMGSFTESG
                     NILGVPEGTIFMGESDTDYQFSDNLHLQSTCPGVGAGTDGTDMGIYGTDSPYKDGAMP
                     HTPYFRKVNIAPGTDANGSLPVSVRVAAQPN"
BASE COUNT          366 a          570 c          500 g          327 t
ORIGIN      
        1 cccttcgtga aacaatgata ccgaccatcc acatgatccc cttcaagcac atcctttttg
       61 gcgccgtggt ggccaccgcc gcccctgcgc ttgcccagca gccacggatc gtggtgcaac
      121 atgccggcag cgtgcaggtg ttctccgatc tgggcgaggc catcaccgcc gcgcagaaca
      181 acgccgaact ctacctgagc ggagggagct tcgttctgcc cggtgggctc gccttgagca
      241 agaccctgca tttcatcgga gcgggcgtgc ataccgacag cactgccgcc agcgggccca
      301 ccatcctgag caccggaacg agtgccaact tccgcctgaa ctcctcggcc agcggcagtt
      361 cgttcagcta catcaccttc aacgtgccgg gcaatggtat ctgcttcggc ttaggcacca
      421 gtgagctgga ccagaccgtg gaaagtgtcg aattccatcg ctgtgtgttc tggcagaccg
      481 tgtcactcgg ggttgttgct ccctgtggct ccacctcatc cttcacggag tgcatcttcc
      541 acagcacgtt gtacggctgc aacgcgacca cgcaggtcac acgctgcatc ttcgactacc
      601 aggccggtac cggagccgag atcagcgcgt tccaaggcgg tgggctcacc atgctcaact
      661 gtgtgtgcct gggcacccgg atcggcgata gtggtgcatc cgtggtgatg aacagcgtgt
      721 tcacgagaac aagcgccccg ttctggcaga gcagcggcat gacgatgacc aacaacctcc
      781 tggtttctcc tgatctcacc agcaacatgg gcagcttcac ggaaagcggt aacatccttg
      841 gcgtaccgga ggggaccatt ttcatgggcg agagcgatac cgactaccag ttcagcgaca
      901 acttgcacct ccaaagcacc tgtccgggtg ttggtgcggg aaccgatggc accgacatgg
      961 ggatctatgg cacggacagt ccgtacaagg atggtgcgat gccgcacacg ccctacttcc
     1021 gcaaggtgaa catagccccg ggtaccgatg cgaacgggag cctgccggta agtgttcgtg
     1081 tggcggcaca acccaactga tcggcgtgcg ggccgtggag cgggcatccc ccaggaatgg
     1141 ggatcgcacc aaggtcctgc acgaccgatg agaacaggag tatggcgttt tgccaggctc
     1201 aacgtgatcc tggggatatg agtattcatc agcactgcgc catcactccc catgcactgt
     1261 aacgctgagt gagcctgtcc tgacgaagga aggaccggag cgctacaaca ctcaccacca
     1321 atggcaccct gagcctgtcg actgcgccta tgcgtagccg ctacggcgaa gcgaggagcg
     1381 ctaccccgat gccatgcacg ctcccatgca ctgtcacgct gagtctgccg aagcgccagc
     1441 actccccacc actgtcacgc tgagcctgcc gaagcgctac gcacaagcgc acaccaagca
     1501 tgttcatgtg agtatcgcgc ttcgacgcgc tcagcgtgac agttcaaagg cagtgtacac
     1561 gtacagtgtt cctgaacatt ctttacccga atgtcccgct gagcgagcct gtcctgacga
     1621 aggaaggacc gaagtgctac cacactccac caatgtcacg ctgagcctgt cgactgcgcc
     1681 tatgcgtagc cgctacggcg aagcgtggag cgctaccccg atgccatgca cgctcccatg
     1741 cactgtcacg ctgagcctgc cga
//
LOCUS       DAOQOC010000721         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_49353_length_1760_cov_3.775953, whole genome shotgun sequence.
ACCESSION   DAOQOC010000721 DAOQOC010000000
VERSION     DAOQOC010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49353_length_1760_cov_3.775953"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PL106_11105"
     CDS             complement(<1..350)
                     /locus_tag="PL106_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA33658.1"
                     /translation="MMKRSLTGLLLLTGLCVLAQKQPAAPAPPVDPVLMTVDGQPVTR
                     GEFEAIYKKNNKDAPVTKEALDEYLDLFINYKLKVRAAEELGMDTVGKFKTELDGYRK
                     QLARPYLIDRNLND"
     gene            572..>1760
                     /locus_tag="PL106_11110"
     CDS             572..>1760
                     /locus_tag="PL106_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNA33659.1"
                     /translation="MASPLFRRKPIGTDTDTPHDGNSGLKRTLGVRDLTFMGVAAVIG
                     AGIFSTVGSASYEGGPGVVFLFIITAVTCGFTALCYAEFAGRVPVSGSAYTYSYVTFG
                     EIVAWIIGWALILEYAIGNVVVANSWSSYFNNILLAFGIHLPTWLATDPTAARDAYES
                     AVQSGADPGTLAWVTAPVIAGQRLIMNVPACLITVLVTALVYVGITESKRMANGMVLL
                     KLAILAAVALVGLFHIKGANYVPFLPNGMSGVLKGVSAVFFAYIGFDAISTTAEECKD
                     PQHDMPRAMIWSLILCTVIYCVTALVLTGMVNYSEFKGVSDPLAFVFERIDMPTFGFI
                     VSISAVVAATSVLLVFQLGQPRIWMSMSRDGLLPPRFGHLHRRFRTPAFATIITGILV
                     GIPA"
BASE COUNT          312 a          539 c          538 g          371 t
ORIGIN      
        1 tcgtcgttca agttgcgatc gatgaggtag ggccgcgcca attgcttgcg atagccgtcc
       61 agttccgtct tgaatttgcc cacggtgtcc atgccgagtt cctcggccgc gcgaactttc
      121 agtttgtaat tgatgaagag gtcgagatac tcatcgagcg cctccttcgt gaccggcgcg
      181 tccttgttgt tcttcttgta gatcgcctcg aattcgccac gcgtcaccgg ctggccatca
      241 acggtcatca ggacggggtc cactggaggg gcgggagcgg caggctgttt ctgggccagc
      301 acgcacagac cggtaaggag caacaggccg gtaagcgaac gcttcatcat gggggggtac
      361 tttctgaaag ggcgtcaaat gtaaccggat cactatcccg agcttcggga ccgacccgtg
      421 ggcgatggcg ttaaaaagat caaggccacg gagcggtgca acgccgacct gcggttccgg
      481 tcgatgggac cgggcgctgc cccaaacacc gtcggcccgt tgagctcaac atccgtgcgt
      541 tccgtatcat cccttcagct acacttgtcg catggcctcg cccctcttcc ggcgcaaacc
      601 catcggaacc gatacggata cgccgcacga cggcaattcg gggttgaaac gcaccctcgg
      661 cgttcgcgac ctcaccttca tgggtgtggc ggcggtgatc ggcgcgggca tcttcagcac
      721 ggtgggctcc gcctcgtacg aaggcggccc gggagtggtc ttcctgttca tcatcaccgc
      781 cgttacctgt ggcttcaccg cgttgtgcta tgcggaattc gcgggccgcg tgcccgttag
      841 cggtagtgcg tacacctaca gttatgtcac tttcggggag atcgtggcct ggatcatcgg
      901 ctgggcgctc attctggaat acgccatcgg caacgtggtg gtggccaaca gttggagcag
      961 ctacttcaac aacatccttc tcgccttcgg catccatctg ccgacctggc tcgccaccga
     1021 ccccaccgct gcgcgcgatg cctacgaatc ggctgtgcaa agcggcgcgg atccgggtac
     1081 gctcgcatgg gtaacagcgc ccgtgatcgc gggtcagcgt ttgatcatga acgtgcccgc
     1141 ctgcctgatc accgtattgg tgacggcact ggtatatgtg gggatcaccg agagcaagcg
     1201 catggccaat ggcatggtac tgctcaaact ggcgatcctg gcggccgtgg cgctggtggg
     1261 cttgttccac atcaaggggg ccaactacgt accgttcctg cccaacggca tgtcgggcgt
     1321 attgaagggg gtgagcgcgg tgttcttcgc gtacatcggt ttcgatgcga tcagtaccac
     1381 ggccgaggaa tgcaaggatc cacagcacga tatgccgcgg gccatgatct ggtcgctcat
     1441 cctctgtacg gtgatctatt gcgtgaccgc gctcgtgctc acaggcatgg tgaactacag
     1501 cgagttcaag ggagtaagcg accccctggc gttcgtgttc gaacggatcg acatgccgac
     1561 cttcgggttc atcgtgagca tcagcgcggt ggtggcggca acgagcgtgc tgctcgtctt
     1621 tcaactgggc cagccacgga tctggatgag catgagccgc gacggactgc tccccccgcg
     1681 cttcggccat ctgcaccgcc gtttccgcac accggcattc gccaccatca tcaccggcat
     1741 ccttgttggc atccccgcgt
//
LOCUS       DAOQOC010000722         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_49567_length_1756_cov_3.490888, whole genome shotgun sequence.
ACCESSION   DAOQOC010000722 DAOQOC010000000
VERSION     DAOQOC010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49567_length_1756_cov_3.490888"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PL106_11115"
     CDS             complement(<1..497)
                     /locus_tag="PL106_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33660.1"
                     /translation="MSNDTLLHEALSRCLGKRLTFAAFRAPGMPITIWAQREPGVETV
                     DGRLLWELNDVFLVAPFELDPAHIAMIRSDVDLSFGEFGSDISALFECRGDERPPMPV
                     EGEGTGQKAYTDAVVAAKAGIAAGRMTKVVLSRTRVARMPEADLIPLFLRACQERPRA
                     FVALVR"
     gene            complement(494..913)
                     /locus_tag="PL106_11120"
     CDS             complement(494..913)
                     /locus_tag="PL106_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016361618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNA33661.1"
                     /translation="MFDEVQLARVNGMFKNTMMETLGIVFVASGPDEVAATMPVEPRT
                     HQPMGMLHGGATVALAEALGSAASYMLIDGDRKAVLGIEVNANHLRTVRSGLVKASGR
                     LMHRGRTMHVWDIRVADERERLVAVCRHTVMIIDRET"
     gene            1010..1708
                     /locus_tag="PL106_11125"
     CDS             1010..1708
                     /locus_tag="PL106_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA33662.1"
                     /translation="MNGEPKRLLLVEDEETLRSTLRLNFELDGYDVTTAVRGSEALER
                     IRGARFDGIVMDVMLPDVDGFTICETMRLEGNSTPVLFLTARTLPQDRIRALRSGDDH
                     LGKPFELEELRLRVQKLVSRGAEHGAVVSPSKRSFGGNSVDFDSYEVSGQRGLHKSLT
                     QREIHLLRLLMDHANEVVSREEILQKVWGYNVFPTTRTIDNFIVAFRKYFEPDPRAPR
                     HFHSIRGVGYKFTP"
BASE COUNT          320 a          586 c          527 g          323 t
ORIGIN      
        1 cgcaccagtg caacgaatgc gcgcggccgc tcctggcagg cacgcaggaa gagcggtatc
       61 aggtccgctt ccggcatacg cgccacccgc gtacgcgaaa gcaccacctt ggtcatccga
      121 cccgccgcga tccccgcttt cgccgcgacc acggcatccg tataggcctt ctgtcccgtt
      181 ccttcccctt ccaccggcat cggtgggcgt tcatcgcccc ggcactcgaa caacgcgctg
      241 atatccgaac cgaactcgcc gaaagaaaga tccacgtcgg accggatcat cgcgatatgc
      301 gcgggatcca gctcgaaggg tgccaccagg aacacatcat tcaactccca caggagccgg
      361 ccgtccaccg tttcaacgcc gggctcccgc tgggcccaga tggtgatggg catacccgga
      421 gcccggaagg ccgcgaaggt gagccgcttc ccgaggcagc gcgatagcgc ttcgtgcaac
      481 agggtatcat tgctcatgtt tcgcggtcga tgatcatgac cgtgtgccgg cacaccgcca
      541 ccaaccgctc ccgctcgtcg gccacgcgga tgtcccacac gtgcatcgtg cggccgcgat
      601 gcatcagtcg gccggaggcc ttcaccaggc cgctgcgcac ggtccgcaga tggttggcat
      661 tcacctcgat accgagtacc gccttccggt cgccatcaat gagcatgtag ctggcagcgc
      721 ttccgagcgc ttcggccagg gccacggtgg ccccgccatg cagcatgccc atgggctggt
      781 gcgtccgcgg ctccaccggc atggtggccg ccacttcatc cgggcccgaa gcgacgaaga
      841 cgatgccgag cgtctccatc atcgtgttct tgaacatgcc gttcacgcgg gccagttgta
      901 cttcatcgaa catcgtgcaa aagtaccctt cgtatcccga tcatgcctca tgcggccctg
      961 atgtcggacc actgctagta tggtatggcg tgcgatcttc gctgcccgga tgaacggcga
     1021 gcccaagcga ctgctgctgg tggaggacga ggagaccctg cgttccaccc tgcgcctgaa
     1081 cttcgaactg gacggttacg atgtcaccac cgccgtgcgg ggcagcgaag ctttggaacg
     1141 catccggggc gcgcgtttcg atgggatcgt gatggatgtg atgctgcccg atgtggacgg
     1201 cttcaccatc tgcgagacca tgcgcctgga ggggaactcg accccggtgc tcttcctcac
     1261 ggcacgcacc ctgccgcagg atcggatccg cgcactgcga tccggggacg accacttggg
     1321 caaaccattc gaactggagg aactgcgctt gcgggtgcaa aaattggtct cccggggtgc
     1381 ggagcacggg gcggtggtca gtccgtccaa gcggagcttc gggggcaact cggtcgattt
     1441 cgactcctat gaggtgagtg gtcaacgcgg tctgcacaaa tcattgacac agcgggagat
     1501 acacctgctt cgcctcctga tggaccacgc caacgaggtt gtgagtcgcg aggagatcct
     1561 gcagaaagta tggggctata acgtctttcc gaccacccgc acgatcgaca acttcatcgt
     1621 ggctttccgg aagtatttcg agccggatcc ccgcgccccg cgccacttcc actccatccg
     1681 gggggtcggc tacaagttca cgccctgatt ttttttccgg ccgggcggca accctgccgg
     1741 aactcgcgcg tcttgg
//
LOCUS       DAOQOC010000723         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_49976_length_1749_cov_3.642267, whole genome shotgun sequence.
ACCESSION   DAOQOC010000723 DAOQOC010000000
VERSION     DAOQOC010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49976_length_1749_cov_3.642267"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PL106_11130"
     CDS             complement(<1..1118)
                     /locus_tag="PL106_11130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNA33663.1"
                     /translation="MKWIIRLLTVCSIIGPLRLAAQWEQVGESGNAYAIALQGSLVFS
                     GLQNDSVYVSADQGDTWSTANTGITNATCWWLESIDGTLYNGTQGGPSFRSTDNGATW
                     VDIGMNGARGFTKHNDTLYVCRWYSGSVHWSVDEGDTWNATEALSGSGGLWPMLSYHG
                     YLFLGGQGGGVYRIQHSTDAWQQMNNGLTGLNIYTFATIGDRLYLGGTNGVWYSDDDG
                     TTWTATGFTGQITYALHTVGSQLYAGGDFGIRFSSDSGATWTLINDGLSAALTARLVD
                     DGTYLYAGVYNGGGVNRRLLSEITSDVHEIVGERTRPSLFPVPARDRVQLTWPGHVPV
                     QYRVRDLSGSLAATGAVGDGSIGVAGLPAGAYFLEVSDR"
     gene            complement(1222..>1749)
                     /locus_tag="PL106_11135"
     CDS             complement(1222..>1749)
                     /locus_tag="PL106_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33664.1"
                     /translation="GMVSAIAAMLLAGWLADRVGKLRVSTMYLALMAAGWLMMGLAPQ
                     LWPHSGYLPAFIYGSKCVVTFYQVATLALAMSLCWARVAATQFTLYMVCNNVGVAAGA
                     ALLGPLRSSLSWSGLFLLFAAALVTLLLAWQFMRLARHKTALERLEQHHAEQVTGSLA
                     SEGSLVPDLGMPGTM"
BASE COUNT          365 a          585 c          512 g          287 t
ORIGIN      
        1 cgatcggaca cctccaggaa gtaggcccct gccggaagtc ccgctacacc gatggaccca
       61 tcacccaccg cccccgttgc ggccaaactg ccggacaggt cgcggacccg gtactgaacc
      121 ggcacatgcc cgggccaggt gagctgcacc cgatcgcggg cagggaccgg gaagagcgag
      181 gggcgcgtgc gctctccaac gatctcatgg acgtcgctcg tgatctccga caacaaccgc
      241 cgattcacgc caccaccgtt gtacacccca gcgtagagat aggtgccatc atccaccagc
      301 cgcgcggtca gcgccgcact caggccatcg ttgatcaggg tccaggtggc accgctgtca
      361 gaagagaacc gaatgccgaa atcaccgccc gcgtagagtt ggcttcccac ggtgtgcagc
      421 gcgtaggtga tctgtccggt gaagcccgtt gccgtccagg tggtgccatc gtcatcagag
      481 taccagaccc cattggtgcc acccagatag agccggtcgc caatggtggc gaaggtgtag
      541 atgttcagac ccgtgaggcc gttgttcatc tgctgccagg catcggtgct gtgctggatg
      601 cggtacacac caccaccttg gccacctaag aagaggtagc catggtacga tagcatcggc
      661 cacagtccac cgctgccgct cagcgcctcg gtggcgttcc acgtgtcacc ttcatccacc
      721 gaccaatgca ccgaaccgga ataccagcgg catacataca gcgtgtcgtt gtgcttggtg
      781 aaaccccgcg cgccgttcat gccgatgtcc acccaagtgg cgccattgtc cgtgctgcgg
      841 aaagacgggc caccctgcgt gccgttgtac aaggtgccat ctatggactc gagccaccag
      901 caggttgcat tggtgatgcc ggtgttggcc gtggaccacg tgtcgccctg gtcggcggaa
      961 acgtaaacac tgtcattctg caagccggag aagacgaggc tgccctgcag tgcgatggcg
     1021 taggcgttac cggattcacc gacttgttcc cactgggccg ccaggcgcaa ggggccgatg
     1081 atgctgcaga ccgtcaacag gcgtatgatc catttcatgc gtgatccgtt tcagacaaag
     1141 taatcgtaaa accaacaaca attgatcgcg cgaccggtca atgcctcgat ggaacggcca
     1201 ccacatccgt agcaccggtc atcacatcgt ccccggcatc cccaggtccg gaaccaggct
     1261 gccctccgaa gccaagctgc ccgtgacctg ttccgcgtgg tgctgttcca ggcgctccaa
     1321 tgcggtcttg tgcctggcca agcgcatgaa ctgccaggcc aacaggagtg tcaccaatgc
     1381 tgcggcgaac aagaggaaca gcccggacca actgagcgag gaacgcaacg gccccagcaa
     1441 tgccgcgccc gccgcaacgc ccacgttgtt gcacaccatg tacagcgtga actgggtggc
     1501 ggccacgcgc gcccagcaca ggctcatggc caaggccaag gtggccacct ggtagaaggt
     1561 caccacgcac ttcgacccgt agatgaacgc cgggaggtag ccactgtggg gccagagctg
     1621 cggcgcaagg cccatcatca gccagcccgc cgccatcaag gccaggtaca tggtgcttac
     1681 acgcaacttg cccacgcggt ccgccaacca gcctgccaac agcatcgccg cgatggcgct
     1741 gaccatgcc
//
LOCUS       DAOQOC010000724         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50272_length_1744_cov_3.113085, whole genome shotgun sequence.
ACCESSION   DAOQOC010000724 DAOQOC010000000
VERSION     DAOQOC010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50272_length_1744_cov_3.113085"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PL106_11140"
     CDS             complement(<1..166)
                     /locus_tag="PL106_11140"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HNA33665.1"
                     /translation="MTRIIECVPNISEGRDRAKIDAIAAAAETVEGVRLLDVDPGKAT
                     NRTVITFVGGP"
     gene            complement(163..954)
                     /locus_tag="PL106_11145"
     CDS             complement(163..954)
                     /locus_tag="PL106_11145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA33666.1"
                     /translation="MESPNAAEKPSLDPIGRSPLSEFLNGFGIFLLVLLVFMGAQSYF
                     LVRHAAGSSPEFAGRGLTMGLLNDPAFQERLTDLTDDGDTVSLVSLWSGLLGLVTLLG
                     FCWWWKRERIINFLGLRPPALKPLAAWIGLFVLVITAIELITLLLPEFDNSFMENVLA
                     SARNKPLLFMGAAIMPAIFEEFFLRGLLYGSLRHVLDKHTAIAIASGVFTIIHVQYPW
                     YLLALYILPLAVLLGYARANTGSIWTAVVLHLINNTVSLALPQLP"
     gene            complement(917..>1744)
                     /locus_tag="PL106_11150"
     CDS             complement(917..>1744)
                     /locus_tag="PL106_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA33667.1"
                     /translation="VEIKSGYGLSFESELKMLRVARRLGEALPLQVRTTLLAAHALPE
                     EYKHDRAGYLCLITDELLPAVADEKLADFIDVFCETNYFTVAEMERVLEAGARFGLPG
                     KVHVNQFTSLGAIRAAVKHGALSVDHLEVMEEADLFALDGAATIPTLLPSCSFFLRIP
                     YAPARAIIDRGCAVALATDHNPGSTPSGNMNLVVSLACIQLRMLPAEAINASTLNAAA
                     AMGLAHELGSITVGKRASLIITRPVPSLAYLPYSFGSDHIDTVIIDGKPERSGEALA"
BASE COUNT          345 a          518 c          588 g          293 t
ORIGIN      
        1 cgggcccgcc cacgaaggtg atcacggtcc ggttggtggc cttgcccgga tccacgtcca
       61 gcaggcggac gccttcaacg gtttccgccg cggcggcgat ggcgtcgatc ttggcacggt
      121 cgcgcccttc gctgatgttg ggcacgcatt cgatgatccg ggtcatggga gttggggcag
      181 cgcgaggctg acggtattgt tgatgaggtg aaggaccacg gcggtccaga tagaaccggt
      241 gttcgctcgg gcatagccca gcaggaccgc cagcggcaga atgtacaggg ccagcaggta
      301 ccaggggtac tgcacgtgga tgatggtgaa gacgcccgag gcgatggcga tggccgtgtg
      361 cttgtccagc acatggcgca aggagccata gagcaggccg cgcaggaaga attcctcgaa
      421 gatggcgggc atgatggccg cacccatgaa cagcagtggc ttgttgcggg cgcttgccag
      481 cacattctcc atgaacgagt tgtcgaactc aggcaacagc agggtgatga gctcgatcgc
      541 ggtgatgacc aggacgaaca ggccgatcca cgcggccaat ggtttcagcg cgggtgggcg
      601 caaaccgagg aagttgatga tgcgctcgcg tttccaccac cagcagaagc ccagcaaggt
      661 gaccagcccc agcaggccgg accacaagga caccaggctc acggtatcgc catcgtccgt
      721 gaggtcggtg agcctttcct ggaaagcagg atcattcaac aaccccatcg tcaggccgcg
      781 cccagcgaat tccggcgaac tccctgccgc atgccgcacc aggaagtacg actgcgcgcc
      841 catgaatacc agcaggacca gcaggaagat gccgaagccg ttgaggaact cggacagggg
      901 cgatcggccg atggggtcaa gcgagggctt ctccgctgcg ttcgggcttt ccatcgatga
      961 tgacggtgtc gatatggtcg ctgccgaagc tgtagggcag gtaggccagc gaaggtactg
     1021 gccgggtgat gatcaagctg gcccgtttgc ccaccgtgat gctgcccagc tcgtgggcca
     1081 gccccatggc ggctgcggca ttgagtgtgg aggcattgat ggcctcggcg ggcagcatgc
     1141 gcaattggat gcaagccagc gagaccacca ggttcatgtt gccgctgggt gtggagccgg
     1201 ggttatgatc cgtggccaat gctaccgcac aaccacgatc aatgatcgca cgggccggtg
     1261 cgtaggggat gcgcaggaag aaggagcagg aggggagaag cgtgggaatg gtcgcggcgc
     1321 cgtccagcgc gaacaggtca gcctcctcca tcacctccag gtggtccacc gaaagcgcgc
     1381 catgtttgac cgctgcccgg atggcgccca ggctggtgaa ctggttcacg tggaccttgc
     1441 ccggcagccc gaagcgtgcc ccggcctcca gcacacgctc catctcggcc acggtgaagt
     1501 agttcgtttc gcagaagacg tcgatgaagt ccgccagttt ttcgtcagcc acggcaggca
     1561 gcaactcatc ggtgatcagg cacaggtagc ccgcgcgatc gtgcttgtac tcctcgggca
     1621 gcgcgtgtgc ggccagcagc gtggtgcgca cctgtaacgg caacgcttcg ccgaggcgcc
     1681 gggccacgcg cagcatcttc aactcactct cgaaggagag cccgtagccg cttttgatct
     1741 ccac
//
LOCUS       DAOQOC010000725         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50352_length_1743_cov_2.593602, whole genome shotgun sequence.
ACCESSION   DAOQOC010000725 DAOQOC010000000
VERSION     DAOQOC010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50352_length_1743_cov_2.593602"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PL106_11155"
     CDS             complement(<1..658)
                     /locus_tag="PL106_11155"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33668.1"
                     /translation="MEHPLPPRPHKRSTGLLGERRSARPEGNQDLATVLRSGQRSFLS
                     VRSGINQVLTLEPLTTVKDLIIVGDGPAAEEIFRYCQDQTVRGYRFRGLFNDRPVEGL
                     LGPQRLGDLEAAKIFAVQNRIDIVYCALPGSQKEAITELLEYCERNTIRFRVIPSVDS
                     FIPVVQAKNLEFHGSVPVSKLRNEPLDKRWNRALKRTFDIVFSLGVIVFVLSWLIPLL
                     A"
     gene            complement(807..1571)
                     /locus_tag="PL106_11160"
     CDS             complement(807..1571)
                     /locus_tag="PL106_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNA33669.1"
                     /translation="MTTPLPKKHVLGVGLSTGSFAQHIKLFAEYGAARRSAYVCCVNA
                     HMCVEAHNDPAFAAVVNGADFATADGMPMLNQLNRRHQLGQERVAGNDIMPALMAEAA
                     AQGLGIYLYGGKQEVLDLIVARAAKDWPTLRISGIESPPYRPLSNAEMEDAAERINAS
                     AAHIVLVSLGCPKQEKWMAAMKGKVNAIMLGMGGAFLLYAGVDSRAPKWMRDLSLEWV
                     YRLALEPGRLWKRYLVTNTTFLWLSLTGESRKRQSV"
BASE COUNT          323 a          530 c          545 g          345 t
ORIGIN      
        1 aggcgaggag cggaatgagc cagctgagta cgaagacgat cacgcccagg gagaaaacga
       61 tgtcgaaggt gcgcttcaag gcacggttcc agcgcttgtc cagcggctcg ttccgcaatt
      121 tgctcaccgg cacactgcca tggaactcca ggttcttggc ctgtaccacc ggtatgaagc
      181 tgtccacact ggggatcacc cggaaccgga tggtgttgcg ttcgcaatac tccagcaatt
      241 cggtgatcgc ttccttctgg ctgccgggca gggcacagta cacgatgtcg atccggttct
      301 gcacggcaaa gatcttcgcc gcttcgaggt cacccaagcg ctgcggaccc aacaagccct
      361 ccaccggccg gtcgttgaac agcccacgga agcggtatcc acgcacggtc tgatcctggc
      421 agtagcggaa gatctcctcg gctgctgggc catcccccac gatgatgaga tcctttaccg
      481 tggtcagcgg ttccagggta agcacctggt tgatgccact gcggaccgac aggaaggagc
      541 gctggccgga gcgcaacacc gtggccagat cctgattgcc ttcaggccgg gcactgcgtc
      601 gctcgccaag cagaccagtg gaacgtttgt gcggacgagg gggtagtgga tgctccacgc
      661 gaagttctga ggattgcggc ccggctgaac gagtgcgtca aatgtagatg gccgtccagg
      721 gggatgtcaa ctttgtcgat aagaatctgc gcttcgtcga ttggtcgggt tagcttgaag
      781 ttttcagacg atggatcagg tcatggtcac accgactggc gttttcggga ttctccggtg
      841 agcgataacc acaggaaggt cgtattggtc acgaggtagc gcttccaaag tcgcccgggt
      901 tccaaggcga gccggtaaac ccattcgagc gagaggtccc gcatccactt cggggcccgg
      961 ctgtccaccc cagcgtacag caggaaagcc ccgcccatgc ccagcatgat cgcgttgacc
     1021 ttgcccttca tggcggccat ccacttttcc tgtttggggc agcccagcga aacgaggacg
     1081 atgtgcgctg cgctggcgtt gatgcgctcg gccgcatcct ccatttcggc attgctgagc
     1141 gggcggtacg gtggcgattc gatcccgctg atgcgcagcg tgggccagtc cttggcggca
     1201 cgcgcaacaa tgaggtccag cacttcctgt ttaccgccat acaggtagat gcccagccct
     1261 tgcgcagcag cttcggccat cagggcgggc atgatgtcgt tgccggcaac acgctcctgc
     1321 cccaactggt gccgccggtt gagctggttg agcatgggca tgccgtcggc cgttgcgaag
     1381 tcggcaccgt tcaccacggc cgcgaaggcg ggatcgttgt gcgcctccac gcacatgtgg
     1441 gcgttcacgc aacagacata cgccgatcgc ctcgccgcac cgtattccgc gaacagcttg
     1501 atgtgctgcg cgaacgatcc tgtggacagg cccaccccga gcacgtgttt cttgggaagc
     1561 ggcgtggtca tgtggtgtgt cccggtcgca ggcgcgggtc tatcaagtca ggtgccacga
     1621 agcgctcact gccgtcctcg taccagcgga tggtctcgtc aacgccctgt tccagcgtgt
     1681 atggtgcttc gcccagcacg gcgatggtgc ttcgacgtgg ccccataccc atggtgatga
     1741 cag
//
LOCUS       DAOQOC010000726         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50394_length_1742_cov_3.114997, whole genome shotgun sequence.
ACCESSION   DAOQOC010000726 DAOQOC010000000
VERSION     DAOQOC010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50394_length_1742_cov_3.114997"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PL106_11165"
     CDS             complement(<1..1090)
                     /locus_tag="PL106_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33670.1"
                     /translation="MIRSLINYTVGFGACLGDPVRRRLLMAHHTRPVPHPADRKEALR
                     AAVGYLIRAQDQGPDKGMGSYHLVHGWSPSYPETTGYIIPTLLAAADHLQWDEPRTRA
                     IQAADWLLSIQHADGGWQGGRIGEGRDPVVFNTAQVIRGLLAVHDATGRAEFLSACTR
                     AGEWILGSQSRDGAWRKENFLGSARVYDTYVSAPILRLHRITGEERYKKAAMMNLEWV
                     LAQQWANGWFSNADNTTKHNDRPITHTIAYTIDGLIECAAHVRHDVMMDRATRTANVF
                     LGRFVQDGWLNGRYDGQWRGSESLITTGCAQLAICWAWLHRITGEERYADGLRRMTEL
                     LMAMQQVNLLGPGAAHGALTGSYPLWGRY"
     gene            complement(1087..>1742)
                     /locus_tag="PL106_11170"
     CDS             complement(1087..>1742)
                     /locus_tag="PL106_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNA33671.1"
                     /translation="TTNDAPPTADRPHVVQCGDVMYDNSLHFAAIAKERSSILRDHGL
                     MPGGFALATVHRDNNTDDPARLNSIFRTLLLLHERHGLDIVLPVHPRTRKMMDALLAP
                     DVKDSLGQSPRFLIVPPAGFLDMIALESNARLVITDSGGVQKEAYFFGKPGVVLRPET
                     EWVELVREGQAVLADADPERITVGFENFLQNGIPEVASLFGDGHAAERICDELLRTW"
BASE COUNT          291 a          563 c          565 g          323 t
ORIGIN      
        1 cataccgccc ccacaacgga taggagccgg tcaaggcacc gtgggctgcg ccagggccca
       61 gaaggttgac ctgttgcatc gccatgagga gctcagtcat gcgccggagg ccatccgcat
      121 agcgctcctc gccggtgatg cggtggagcc aggcccagca gatggccagt tgtgcgcatc
      181 cggtggtgat caggctttcg ctgccccgcc attgcccatc gtaccggcca ttcaaccaac
      241 cgtcctgcac gaagcggccc aggaagacgt tcgcggtgcg cgttgcacgg tccatcatca
      301 cgtcatgccg gacgtgcgcg gcgcattcga tcagcccgtc gatggtgtag gcgatcgtat
      361 gcgtgatggg ccggtcgttg tgcttcgtgg tattgtcggc gttgctgaac cagccgttgg
      421 cccactgttg cgccagcacc cactccaggt tcatcatggc cgccttcttg taccgttcct
      481 caccggtgat gcggtggagc cgcaggatgg gtgcgctcac gtatgtgtcg tacacacggg
      541 cgctgcccag gaaattctcc ttgcgccagg ctccgtcccg cgattgggaa ccgaggatcc
      601 attccccggc acgcgtgcat gccgacaaga actccgcccg tccggtcgca tcatgcacgg
      661 cgagcagccc gcggatcact tgtgcggtat tgaagaccac cgggtcccgg ccctcaccga
      721 tgcggccccc ctgccagccg ccgtccgcat gctggatgga gagcaaccag tccgccgctt
      781 ggatcgctcg ggtacgtggc tcgtcccatt gcagatgatc cgccgccgcg aggagcgtcg
      841 ggatgatgta gcctgtggtt tccggatagg acgggctcca gccgtgtacg aggtgatagc
      901 tgcccatgcc cttgtccgga ccctggtcct gggcgcggat cagatagccg acggctgcgc
      961 gcagtgcttc cttccggtcc gctggatgcg gcacaggcct cgtgtggtgc gccatcagca
     1021 gtcgccgccg gaccgggtcg cccaggcagg cgccgaagcc aacggtataa ttgatcaggg
     1081 agcggatcac caggtccgga ggagttcgtc gcagatccgt tcagcggcat ggccatcgcc
     1141 gaacaacgag gccacttccg ggatcccgtt ctgcaggaag ttctcgaagc ccacggtaat
     1201 gcgctccgga tccgcatcgg ccagcacggc ctgcccttcg cgcacgagtt ccacccattc
     1261 ggtctcgggc cgtagcacca ccccgggttt cccgaagaaa taggcctctt tctgcacacc
     1321 accgctgtcg gtgatcacca ggcgcgcgtt gctttccagg gcgatcatgt ccaggaagcc
     1381 ggccggagga acgatcagga accgcgggct ctggcccagt gagtccttca catcgggagc
     1441 gagcagggcg tccatcattt tccgggtacg cggatgcacg ggcagtacga tgtccagccc
     1501 atggcgttcg tgcaacagga gcagggtgcg gaagatgctg ttgagccgag cggggtcatc
     1561 ggtattatta tcgcggtgaa cggtggccag ggcgaatcct cccggcatga gcccatggtc
     1621 ccgcagaatg gacgaacgct ccttcgcgat ggccgcgaag tgcaggctgt tgtcgtacat
     1681 cacatcgccg cactgcacca cgtgcggccg gtcggcggtg ggcggggcat cgttcgttgt
     1741 gg
//
LOCUS       DAOQOC010000727         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50506_length_1740_cov_3.219585, whole genome shotgun sequence.
ACCESSION   DAOQOC010000727 DAOQOC010000000
VERSION     DAOQOC010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50506_length_1740_cov_3.219585"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PL106_11175"
     CDS             complement(<1..654)
                     /locus_tag="PL106_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNA33672.1"
                     /translation="MATSAIHVFAHWSGMDAPQHMGELRVARSRGKEVFVFSYDQVWL
                     KGGGRFALDPALRLFTGPQYVQEDQPNFGLFLDTAPDRWGRMLMQRREALIARKQQRP
                     VRALAESDYLLGVHDRSRMGALRFKLDEQGAFLADDSDKPIPPMARLRELEAACLAIE
                     TGAGRDQEKWLNLLLAPGSSLGGARPKASVIDPSGALWIAKFPSRSDDLDKGAWEWVT
                     "
     gene            complement(654..1004)
                     /locus_tag="PL106_11180"
     CDS             complement(654..1004)
                     /locus_tag="PL106_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA33673.1"
                     /translation="MPHLLPSLARHLDVLGANIRLARLRRHLSTVQVAERAGIARQTL
                     YHVERGDPAVSLLNYALVLHVLGLEKDLAGIAADDVLGRKLQDQALTTPKRAPRQRGQ
                     AHPPPRMHAKPGNR"
     gene            1206..>1740
                     /locus_tag="PL106_11185"
     CDS             1206..>1740
                     /locus_tag="PL106_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNA33674.1"
                     /translation="MSLTDEIARRRTFAIISHPDAGKTTLTEKILLFSGQIQLAGAVR
                     SRKTNRSTVSDWMAMERERGISVTTSVMSFEYPHPDGEGRPPAQVNLLDTPGHADFGE
                     DTYRVLTAVDGALMVIDGAKGVEDRTEKLIEICRLRDTPVITFVNKLDRECMDPLELL
                     DDIESKLRMACVPMTWPI"
BASE COUNT          318 a          541 c          592 g          289 t
ORIGIN      
        1 ggtcacccac tcccatgcgc ccttgtcaag gtcatcgctg cggctcggga acttggcgat
       61 ccacaaggca ccactcgggt cgatcacact ggccttgggc cgtgctccgc caagtgatga
      121 accgggcgcg aggagcaggt tcaaccactt ctcctgatcg cgaccggcac ctgtttcaat
      181 ggcgaggcac gcggcttcca gttcgcgcag gcgtgccatg ggcgggatgg gtttgtcact
      241 gtcatccgcc aggaaggcgc cctgctcatc caacttgaaa cgcaaggccc ccatgcgcga
      301 ccggtcatgc accccgagca agtagtcgct ttcggccagg gctcgcacgg gcctttgctg
      361 ctttcgtgcg atcaatgctt cacggcgctg cataagcatg cggccccagc ggtccggggc
      421 ggtgtccagg aacaggccga agttgggctg gtcttcctgg acatactgcg ggccggtgaa
      481 gagccgcagc gcgggatcca gtgcgaagcg cccaccgcct ttcagccaga cctgatcata
      541 ggagaagacg aacacttcct taccgcgcga acgggccacg cgtaactcac ccatgtgctg
      601 gggtgcatcc atgcccgacc aatgggcgaa gacatgtatg gcggatgtgg ccatcagcgg
      661 ttcccgggtt tggcgtgcat gcgtggtggc gggtgtgcct gaccgcgctg acggggcgcg
      721 cgtttgggcg tggtgagcgc ctggtcctgg agtttccggc cgagcacatc atctgccgca
      781 attcccgcca ggtccttttc caggccgagc acatggagca ccaaggcata gttgagcaga
      841 ctcaccgccg ggtcgccacg ctccacgtgg tagagggttt gtcgggcgat gcccgcgcgt
      901 tcggccacct gcacggtgct caggtgtcgg cgcagccggg ccaagcggat gttggcaccc
      961 agaacgtcga gatggcgggc cagggagggc agcaggtggg gcatggcgga acgaatgtgc
     1021 gctaaagtag gcaatataca gactatttgt acagcataac gtacattcgg tgaaaatgat
     1081 cacctggtca caacagcctc ccctcggatc ggtcagggcg cgggtgcgtg gagcaacagg
     1141 caaggcacgt tgcctggggg cctgtacatt ccccccgcca tagcaccagc cagggcggga
     1201 cccccatgtc cctcaccgac gagatcgcgc gccgccgcac cttcgccatc atcagccacc
     1261 ccgacgcggg caagaccacg ctcacggaga agatcctgct cttctcgggg cagatccagc
     1321 tcgccggggc ggtgcgatcg cgcaagacca accgctcgac cgtgtcggac tggatggcga
     1381 tggagcggga gcgcgggatc tcggtgacga cgtcggtgat gagcttcgag tacccgcacc
     1441 cggacgggga ggggcggccg cctgcgcagg tgaacctgct cgatacgcca ggtcacgcgg
     1501 acttcggcga ggacacctac cgggtgctca cggcggtcga cggggcgctg atggtgatcg
     1561 acggggcgaa gggggtggag gaccggacgg agaagctgat cgagatctgc cggctccgcg
     1621 acacgccggt gatcaccttc gtgaacaagc tcgaccgcga gtgcatggac ccgctggagc
     1681 tgctggacga catcgagagc aagctgcgga tggcgtgcgt gccgatgacg tggccgatcg
//
LOCUS       DAOQOC010000728         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50602_length_1738_cov_3.453951, whole genome shotgun sequence.
ACCESSION   DAOQOC010000728 DAOQOC010000000
VERSION     DAOQOC010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50602_length_1738_cov_3.453951"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PL106_11190"
     CDS             <1..1029
                     /locus_tag="PL106_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA33675.1"
                     /translation="LLMKLTAGGTDLEGWRMLPRIYAACLVAMAVIFFLFTTNRRPEG
                     DARTMRRMLEPLRTVRVWRFGLYYFLVFGCFVAFAQWLVPYYVNVYAVSLVTAGVFAS
                     MFSFPSGVIRALGGWLSDRWGARKVMYWVLGSSVAICLLLVVPRMEVTTPGKGVMSTL
                     NGRVTYVSDTLVQVNGTAFAVRQEPPSEMHGMHERRIWPTKRTWQNPVVQVGDEVRRK
                     QLLAQGVTRIHFSAHMMVFTVLVILVGIVWGIGKAAVYKHIPEYFPDQVGVVGGMVGV
                     LGGLGGFLGPILFGYLLEATGLWTSSWLFLLLVSLACLLWMNHVVNLIQRRAAPEVNK
                     TMEVLRIV"
     gene            1034..>1738
                     /locus_tag="PL106_11195"
     CDS             1034..>1738
                     /locus_tag="PL106_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA33676.1"
                     /translation="MATWIENWNPEDNTFWREKGERVAWRTLWVTTAALTLSFATWFM
                     MSAIVTKLPGIGFTFTTDQLFWLAAMPGLAGGTLRIVHTFLIPLYGTRHTVTFSTLIK
                     LIPVIGIGLAVMDPTTPFWVFMVLALTAGFGGGDFSSYMPSINLFFPRRLKGTALGIQ
                     AGIGNFGVSLAQFMTPLMLGVAMVGAPQVFNKVDPISKAVLGTSEIHLQSAAFWYVPL
                     ILIVAVPGVIAFVFGYF"
BASE COUNT          264 a          549 c          558 g          367 t
ORIGIN      
        1 ttgctgatga aactgaccgc cggcggaacg gacctggagg gctggcgcat gctgccccgc
       61 atctatgccg cctgtctggt agcaatggcc gtgatcttct tcctcttcac cacgaaccgg
      121 agaccggagg gtgatgcgcg caccatgcgc cggatgctgg agcccctgcg cacggtgcgc
      181 gtgtggcgct tcggcctgta ctacttcctg gtgttcggct gtttcgtggc ctttgcgcaa
      241 tggctggtac cttattatgt gaacgtgtac gcggtctcac tggtcacggc cggggtgttc
      301 gcttccatgt tcagcttccc ctccggggtg atccgcgccc tgggcggctg gctgagcgac
      361 cgctggggcg cgcgtaaagt gatgtactgg gtgcttggct cctccgtggc gatctgcttg
      421 ctgctggtgg tgccgcgcat ggaagtgacc acgcccggca agggcgtgat gagtacgttg
      481 aacggcaggg tgacctatgt ctccgacacg ctggtgcagg tgaacggaac agccttcgcg
      541 gtgcggcagg aaccaccctc ggagatgcac ggcatgcatg aacgccgcat ctggccgacg
      601 aagcgcacct ggcagaaccc ggtggtgcag gtgggcgatg aggttcgtcg caagcagttg
      661 ctcgcacaag gtgtcacgcg catccatttc tccgcccaca tgatggtctt caccgtgctg
      721 gtgatcctgg tgggcattgt ctggggcatc ggcaaggccg ccgtgtacaa gcacatcccc
      781 gagtacttcc ccgaccaggt gggcgtggtg ggcggcatgg tgggcgtgct cggcgggctg
      841 ggcggtttcc tcggccccat ccttttcgga tatctgctgg aggcgacggg gctctggacc
      901 agctcctggc tcttcctgtt gctcgtctcc ctggcgtgcc tgctttggat gaaccacgtg
      961 gtgaacctga tccagcgtcg cgccgccccg gaagtgaaca aaacaatgga ggtcctcagg
     1021 atcgtctgaa cccatggcca cctggatcga gaactggaac cccgaggaca ataccttctg
     1081 gcgtgagaag ggcgaacgcg ttgcctggcg cacactgtgg gtgaccaccg cagcgctcac
     1141 cctttccttc gctacctggt tcatgatgag cgccatcgtg accaagctgc cggggatcgg
     1201 cttcaccttc accaccgacc agctcttctg gctggcggcc atgcccgggc tggccggcgg
     1261 cacgctgcgc atcgtgcata cgttcctgat cccgctctac ggcacgcgcc acaccgtcac
     1321 cttctccacg ctcatcaagc tcatcccggt gatcggcatc ggactggccg tgatggaccc
     1381 gacgacgccc ttctgggtgt tcatggtgct ggccctcacc gctggcttcg gcggtggcga
     1441 cttcagcagt tacatgccca gcatcaacct gttcttcccg cggcggctga agggaacggc
     1501 cctgggcatc caggccggca tcggcaattt cggtgtgagc ctcgcgcagt tcatgacgcc
     1561 cctgatgctt ggcgtggcca tggtgggcgc gccgcaggtc ttcaacaagg tcgatccgat
     1621 ctccaaggcg gtgctgggca cctcggagat ccacctgcag agcgccgcct tctggtacgt
     1681 accactgatc ctgatcgtgg ccgtgccggg cgtcatcgcg ttcgtgttcg gctacttt
//
LOCUS       DAOQOC010000729         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50786_length_1735_cov_3.107738, whole genome shotgun sequence.
ACCESSION   DAOQOC010000729 DAOQOC010000000
VERSION     DAOQOC010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50786_length_1735_cov_3.107738"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PL106_11200"
     CDS             complement(<1..532)
                     /locus_tag="PL106_11200"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA33677.1"
                     /translation="MNKQPDTRRIPTLLALLPALLLGLRLCAQDPVFTQFSSNPLHLN
                     PAFTGDHCWRVVMNSRTQWSQLSGRYQTSAMSVDHGFRNWGVGIQAMYDGGAAYTFRD
                     LTISGLLSYRTRLGRSLWTSMGFRFNYQQRVIDPNKLQFRDQIETHDGMGFIYRSGED
                     IFTTRLNGTDLGAGLLL"
     gene            816..>1735
                     /locus_tag="PL106_11205"
     CDS             816..>1735
                     /locus_tag="PL106_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33678.1"
                     /translation="MGTTLLRRFGSLLVLLVLTTAASGQCGITVEAGAPFCAGESVSL
                     GVDAGPAGTTYAWSVPGCPGCLQGANTATPVFTAPPPSDGTDFTVNLTVTEPGDTPCV
                     ATSVQIPMEVTPFANISVNGYSATAFNGLMVFADCTDPASVDFAVQDACQQCTGSALS
                     LSMNAGAYSAVGTGWSQNVTVAGDMQTLTYVVNNGACPDTGFFGLFVGNTQAPPGSFT
                     LDQGQVTTICSDGSLSFGLLPGLGVVGTTYTVYNGESTTPYGQYQYPPPNAIDFVYGS
                     SSCGNSTPFITCPGGQACLASPVGSYSIAC"
BASE COUNT          318 a          522 c          535 g          360 t
ORIGIN      
        1 aaagcagtaa gccggcccca aggtcggtgc cgttcaagcg cgtggtgaag atgtcctcgc
       61 ccgatcggta gatgaagccc atcccatcgt gcgtttcgat ctgatcgcgg aattgcagct
      121 tgttcgggtc gataacgcgc tgctggtagt tgaagcggaa gcccatgctg gtccacaggc
      181 tgcggcccaa tcgggtccgg tacgagagca ggccactgat cgtcaggtcc cggaaggtgt
      241 acgcggcccc accatcgtac atggcctgaa tgcccacgcc ccaattgcgg aagccatggt
      301 ccacactcat ggccgaggtc tggtagcgac cggacagctg gctccattgt gtacggctgt
      361 tcattacaac cctccagcaa tggtcgcccg tgaatgccgg gttcaaatgc agcgggttgc
      421 tgctgaactg cgtgaaaacg gggtcctggg cgcagagccg cagaccgagg agcagtgcgg
      481 ggagcagtgc gaggagcgtg gggatgcgtc gtgtatccgg ttgtttgttc acatgacccg
      541 ttgtggtccg ggcccgatcc ccgtacgcgc gtggaactga ataccgacaa acagtgatct
      601 tccaaccgcc ttccgtggaa ttccgctcgt tgtgcatcct gacaaccgcg aagatcggga
      661 gcggtagcat acttcaaatg gtttttcgcc aaattgccga cctttccatc gacaacgtgc
      721 cgttcgagac cgggatcatc ccacctgacc accacctggc acctcctggt cgttcgtcgc
      781 catcatccgt cccgggtcat cgaccaaggc tgttgatggg aacgacactg ctacggcgct
      841 ttggatccct gctggtcctg ttggtcctga ccacggcggc atccggccaa tgcgggatca
      901 cagtggaggc gggtgcgccg ttctgtgcgg gcgagtccgt gtccttgggt gtggatgcgg
      961 gtcctgcggg gaccacgtat gcatggtcgg tccccggatg cccgggatgc ttgcagggcg
     1021 ccaataccgc gaccccggtg ttcaccgccc caccgcccag tgatggcacc gacttcaccg
     1081 tgaacctcac ggtcactgaa cccggggata caccatgcgt tgccacatcg gtccagatcc
     1141 ccatggaggt cactccgttc gccaatatca gtgtgaacgg ttattccgcc acggcgttca
     1201 acggcctcat ggttttcgcc gactgtacgg atcccgcctc ggtggatttc gcggtacagg
     1261 atgcatgcca acaatgcacc ggcagtgcgc tttcgttgag tatgaacgcc ggtgcgtaca
     1321 gcgcggtggg aacaggctgg tcccagaacg tcacggtcgc gggtgatatg cagacgctca
     1381 cgtacgtggt gaacaacggc gcatgcccag atactggatt tttcggattg ttcgtgggga
     1441 atacgcaggc tccgcccggg tccttcacat tggaccaggg acaggtgacc acgatctgca
     1501 gtgatgggtc cctctcgttc gggctgttgc ccgggcttgg tgtggtggga acgacctata
     1561 cggtgtacaa cggggagtcg accacaccgt atggacagta ccagtatcca ccacccaacg
     1621 cgatcgactt cgtgtacggt tcatcgagct gcggcaattc aacgccgttc atcacctgtc
     1681 cgggcgggca ggcctgtctc gcctcgccgg tcggcagcta ttccatcgcg tgttg
//
LOCUS       DAOQOC010000730         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50798_length_1735_cov_2.650595, whole genome shotgun sequence.
ACCESSION   DAOQOC010000730 DAOQOC010000000
VERSION     DAOQOC010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50798_length_1735_cov_2.650595"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PL106_11210"
     CDS             <1..271
                     /locus_tag="PL106_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HNA33679.1"
                     /translation="VGYLVGTRTGAFTYNLTHHKGIALLLMLIGLVVEMANMLANVQS
                     GYFLAGLILYGHSALDRMLGFGLKFGDHFQHTHLGWIGGSRKTDN"
     gene            351..914
                     /locus_tag="PL106_11215"
     CDS             351..914
                     /locus_tag="PL106_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNA33680.1"
                     /translation="MRILLLDNYDSFTWNLHHLLAPYASVDVFLNDGITVNEAARYDR
                     IVLSPGPGLPREAGILMDLLRALMPTHPILGVCLGMQAIVETCGGSLYNQTRVMHGME
                     VLCFPEEPHDPLFSGLSSPFNVGLYHSWAADPTTLPADLRTTARSAEGVIMAVRHHQF
                     NTCGVQFHPESVMTPMGGRVIANWMRS"
     gene            1008..>1735
                     /locus_tag="PL106_11220"
     CDS             1008..>1735
                     /locus_tag="PL106_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33681.1"
                     /translation="MILPITLVCMATVVNGQSMLQRALNNDTMPNLVPNPGFELVKRQ
                     QCAWTQEARKFGEEVMIAWNSPTETTPDHFSLKADPDCWSHPAKKSKGRATPHSGDAM
                     SGIKIYGKGNTPTYWHEYLQIELPEALEAGRLYVCEFWTLRAVFSNVASNNMGMYLSP
                     VAVKTRDCLPLYFPAQVREEDVIRNGWHKVSGVIEAKGDERFVLIGNFCTDQLTRNEK
                     MPEGERGAYYFIEVFPASREERKLR"
BASE COUNT          346 a          571 c          507 g          311 t
ORIGIN      
        1 ggtcggttac ctggtcggga cacgtaccgg tgcgttcacg tacaacctca cacatcacaa
       61 aggcatcgcc ctcctgttga tgctcatcgg tcttgtcgtc gagatggcga acatgctcgc
      121 caacgtgcag agcgggtatt tcctggccgg cctgatcctg tacggacatt cggccctgga
      181 ccggatgctc ggcttcggac tgaaattcgg tgatcacttc cagcacacgc acctgggctg
      241 gatcggcgga tcccgaaaaa ccgacaattg agtatcatcg ccaccggtcg tgctccggaa
      301 tgcacggacc tccccggcac cttggaccat catgcagctc cgatcccgcg atgcgtatcc
      361 tgctcctgga caattacgat tcattcacgt ggaacctgca ccacctgctg gcgccgtacg
      421 cttcagtgga cgtgttcctg aatgacggga tcacggtgaa cgaggcagcg cggtacgatc
      481 ggatcgtgct gtcgcccggc ccgggcctgc cgcgtgaagc ggggatcctg atggatctgc
      541 tgcgcgcgct catgcccacc catcccattc tcggggtctg cctcggcatg caggcgatcg
      601 tcgagacctg cggtggttcg ctgtacaatc agacccgtgt gatgcacggc atggaggtcc
      661 tttgtttccc ggaggaacca cacgacccgc tcttctccgg tctgtcctcg ccattcaacg
      721 tgggcctgta ccacagttgg gccgcggacc cgacaaccct tcccgcggac ctccgcacca
      781 ccgcgcgcag cgccgagggc gtgatcatgg ccgtgcggca ccaccaattc aatacctgcg
      841 gggtacaatt ccaccccgaa agcgtgatga cgccgatggg cgggcgcgtc atcgccaact
      901 ggatgcggag ctgaccggtc gctccatcca cgatcggctt gttgatcctt gcactgaagt
      961 gccgggagaa ccgcgaccgg cgcaggttat ttctcggcat gatccgaatg atccttccca
     1021 tcacactcgt gtgcatggcc accgtcgtga acgggcaaag catgctccag cgcgccctga
     1081 acaacgacac catgcccaac ctggtgccca acccgggctt cgaactggtg aagcggcaac
     1141 aatgcgcgtg gacgcaggag gcacgcaagt tcggtgaaga ggtgatgatc gcatggaatt
     1201 cgcccaccga gaccacaccc gaccacttca gcctgaaggc cgatcccgac tgctggagcc
     1261 atcccgccaa gaagtccaaa ggacgcgcga ccccgcacag cggcgatgcc atgtccggca
     1321 tcaagatcta cggcaaaggc aacaccccca cctactggca cgagtacctc cagatcgaat
     1381 tgcccgaggc gctcgaagca ggccgcttgt acgtgtgcga attctggacc ctgcgcgcgg
     1441 tcttcagcaa cgtggcctcg aacaacatgg gcatgtacct gagcccggtg gccgtgaaga
     1501 cgcgcgactg cctgccgctc tacttccccg cacaggtgcg cgaagaagac gtgatccgca
     1561 atggctggca caaggtgagc ggggtgatcg aggcgaaggg cgatgaacgt ttcgtgctca
     1621 tcggcaactt ctgcaccgac caactcacac ggaacgagaa gatgcccgag ggcgagcgcg
     1681 gcgcctacta cttcatcgag gtcttccccg cctcgcgcga ggaacgcaag ctgcg
//
LOCUS       DAOQOC010000731         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50846_length_1734_cov_3.066706, whole genome shotgun sequence.
ACCESSION   DAOQOC010000731 DAOQOC010000000
VERSION     DAOQOC010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50846_length_1734_cov_3.066706"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PL106_11225"
     CDS             <1..710
                     /locus_tag="PL106_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33682.1"
                     /translation="GTGVPLGVLPAGTGNDLYTLDCICAGLLIDCAGVPGGTTLPGSP
                     CDDLNPLSIGDVYNAQCQCAGILPNDCAGVPGGPAQPGTSCDDGNADTGNDVYNAFCT
                     CVGLLIDCQGTPGGLALPGTPCDDGNTNTINDAYDLGCACVGDINTEVSTAGTGPDSF
                     SVQPNPSNGSFAFVNPTQVPTQVTVLDATGRKVLGMRNVTARTTWMDIPGLGAGVYYL
                     HASASDGQQVIKVIIEH"
     gene            complement(785..1186)
                     /locus_tag="PL106_11230"
     CDS             complement(785..1186)
                     /locus_tag="PL106_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33683.1"
                     /translation="MEKRTKDKPRTLKTPPRTAEYTMHVEEKDGKEVLVCTVGKAVLL
                     QDLRGIDDLYVMLKKHGDRMDLGGADEQKPAKDGTVEAWGRSEKNPVKGWYGLKKGLR
                     GRFGVYLPPLMEALGMCELSHEAKGNKMRAK"
     gene            complement(1236..>1734)
                     /locus_tag="PL106_11235"
     CDS             complement(1236..>1734)
                     /locus_tag="PL106_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33684.1"
                     /translation="NLLPPLLIMTPAATFAYNASTTRTTFLRETTVSATIKADEAIIW
                     ALLTNASDHPRWNSTIASLQGTFAPGEKIVLKSTLEAKRSFKLKVKEFQSAKKLVWGD
                     GKVQRTFTLKNNSDGTVTFTMTERIGGLMFPMHAKYIPSFDDSFNTFAADLKKQIDPI
                     QNKAL"
BASE COUNT          292 a          526 c          533 g          383 t
ORIGIN      
        1 cgggcaccgg ggtaccgctg ggcgtgctgc cagcgggtac cggcaacgat ctctacacct
       61 tggactgcat ctgcgccggc cttctgatcg attgcgcggg tgttcctggc ggaaccacat
      121 tgcccggctc gccatgcgac gacctcaatc cgctcagcat cggggacgtt tacaatgccc
      181 aatgccagtg cgcgggtatt ctcccgaacg actgcgccgg tgtgcccggt ggccctgcac
      241 agcccggtac ttcctgcgat gatggcaatg cggataccgg caacgatgtc tacaatgcct
      301 tctgcacctg cgttggcctc ctcattgact gccaaggcac gcctggcggg ctggctttgc
      361 ccggcacacc ttgcgatgac gggaatacca acacgatcaa cgacgcgtac gacctcggct
      421 gtgcatgcgt tggcgacatc aatacggagg tcagcaccgc aggaacggga ccggacagct
      481 tcagcgtaca gccgaacccg agcaacggaa gctttgcttt cgtgaatccg acgcaggtac
      541 cgacgcaagt gaccgtgctt gatgcgaccg gtcggaaggt gctcggcatg cgcaacgtca
      601 cggcacgcac gacctggatg gatatcccgg gccttggcgc cggtgtttac tacctgcacg
      661 cttcggccag cgatggtcag caggtgatca aggtgatcat cgagcactga caggccgctt
      721 cacgaacgaa cggccccgag cgcatcgggg ccgttcgtcg ttcagcgcac tcggcatcca
      781 ttcatcactt cgcccgcatc ttgttcccct tcgcctcgtg cgagagctcg cacatgccca
      841 gcgcttccat caacggcggc aggtacaccc cgaagcgacc tcgcagtcct ttcttcaagc
      901 cgtaccaacc tttcacgggg ttcttctcgc tgcggcccca tgcttccaca gtgccatcct
      961 tcgcgggctt ctgctcgtcg gcgcctccga ggtccatccg atcgccgtgc ttcttcagca
     1021 tcacgtacag gtcgtcgatg ccccggaggt cctggagcag aacggccttt cctacggtgc
     1081 acacgagcac ctccttgcca tccttctctt ccacgtgcat ggtgtactct gccgtgcgag
     1141 ggggcgtctt cagggtccgg ggcttgtcct tggttcgctt ttccattgca gtggtcgtgt
     1201 gttgttgggg cgggatatgt cccgaccgtc tgggatcaga gggctttgtt ctggatcgga
     1261 tcaatttgct tcttcaggtc ggctgcgaag gtgttgaagc tatcatcgaa cgatgggatg
     1321 tatttggcat gcatcgggaa catgaggccg ccgatgcgct cggtcatggt gaaggtcacc
     1381 gtgccgtcgc tgttgttctt caaagtgaag gtgcgctgga ctttgccgtc gccccacacc
     1441 agcttcttcg ccgactggaa ttccttcacc ttcaacttga agctgcgctt ggcttcgagg
     1501 gtgctcttca gtacgatctt ctcgcccggg gcgaaggtgc cttgaaggga ggcgatggtg
     1561 ctgttccaac gcggatggtc gctggcgtta gtgagcagcg cccagatgat ggcctcatcg
     1621 gctttgatgg tggcgcttac cgtggtctcc cggaggaagg tggtcctcgt ggtggaagca
     1681 ttataggcga aagtggcggc gggggtcatg atcagaaggg gggggaggag gttc
//
LOCUS       DAOQOC010000732         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_50887_length_1733_cov_3.598927, whole genome shotgun sequence.
ACCESSION   DAOQOC010000732 DAOQOC010000000
VERSION     DAOQOC010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50887_length_1733_cov_3.598927"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..904
                     /locus_tag="PL106_11240"
     CDS             191..904
                     /locus_tag="PL106_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33685.1"
                     /translation="MRRTILLLMLVAATALARAQDVTVHGQVQGGDGERTFYDLMIVN
                     RRLRTGNFGNVDGSFTVSALRNDTLLIGAGGYVTRTVPLSEYTDAELAALKVTLRPWE
                     IQLKPVAVLPERTLKEIQADIDKLGYDEKDYRTAGVNALQSPITFLYQEFSRRERSKR
                     MVAELRNEDAKRALLRELLYKYVEYDIINLSNDSFDDFIDFCAVPDEVIKGLSQYDFL
                     MYVKKKYELYTSLGPTRRH"
     gene            904..>1733
                     /locus_tag="PL106_11245"
     CDS             904..>1733
                     /locus_tag="PL106_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33686.1"
                     /translation="MRRSIAILCVVLDALMVRAQTDVDTIVNVYQLGEVVVHGGAQGL
                     QLEGFFRQVMEDTTFYHAFLNTKYHAHAVESELRVRDKKSRETAGLYRKGRMVRTGPM
                     AELVLDSVREIGKLRDRNGGIRFLTAEMYDDVFFPKGQWRASNRIAAREQEISRASRF
                     DKYKSELKKFMFNPGQEIASVPFIGDKLDIFDPDMAPLYDYSVGSGFRDGRPCWLFTA
                     VARDQVGGRPADEDDTVIKRMLTWFDQGTMNVLAREYRMRSSGILLDFDLLARIDEDT
                     R"
BASE COUNT          356 a          483 c          580 g          314 t
ORIGIN      
        1 ggacagaacc cggcttacag gtgcgcaggt acttccacga agggtcttcc gttgatcgga
       61 gggcccttcg ccattctggt acggtcgata gcgacgcggg ccggaaccaa gccatcgccc
      121 ggatgccggt ccatgtgaac ggctgtgaag tggcgcggtc cttggaaagg ggcgggctac
      181 tttcggcccg atgagaagaa cgatcctgct gttgatgctc gttgccgcca ccgcgctggc
      241 acgggcacag gacgtcacgg tgcatggcca ggtgcagggc ggcgatggtg aacggacgtt
      301 ctatgacctg atgatcgtga acagacggtt gcgcacgggc aacttcggca acgtggacgg
      361 cagcttcacg gtgagcgccc tgcgcaatga cacgttgctc atcggcgcgg gtggttatgt
      421 gacgcggacc gtaccgctga gcgagtatac cgacgccgaa ctcgcggcat tgaaggtgac
      481 cttgcggcca tgggagatcc aactgaagcc cgtggccgta ctgcccgaac gcacgttgaa
      541 ggagatccag gccgacattg acaagctggg ctacgatgaa aaggactacc gcacggccgg
      601 tgtgaacgct ttacagagcc cgatcacctt cctgtaccag gagttcagcc gtcgggaacg
      661 cagcaagcgc atggtggccg agttgcgcaa tgaggatgcc aagcgcgccc tgctccgtga
      721 gttgttgtac aagtacgtgg agtacgacat catcaacctg agcaacgaca gctttgacga
      781 cttcatcgat ttctgtgcgg tgcccgatga ggtgatcaag gggctttcgc agtacgactt
      841 cctcatgtac gtgaagaaga agtacgaact gtacaccagc ttgggcccca cccgcaggca
      901 ctgatgcgcc gctcgatcgc gatcctgtgc gtggtgctgg atgccctcat ggtccgggcg
      961 caaacggacg tcgacaccat cgtgaacgtg taccagctgg gcgaggtggt ggtgcatggc
     1021 ggagcgcagg gactccagtt ggaaggcttc ttccggcagg tgatggagga caccacgttc
     1081 taccatgcat tcctgaacac caagtatcat gcgcacgccg tggagagcga actgcgcgtg
     1141 cgcgacaaga aaagccggga gaccgcaggc ctctaccgga aggggcgtat ggtgcgcacc
     1201 gggcccatgg ccgaattggt gctggacagc gttcgcgaga tcggcaagtt gcgggatcgg
     1261 aacggcggga tccgcttcct cacggccgag atgtacgatg atgtgttctt ccccaagggg
     1321 caatggcgag cgagcaaccg catcgctgcg cgcgagcagg agatcagccg ggccagccgg
     1381 ttcgacaagt acaagagcga actgaagaag ttcatgttca acccgggcca ggagatcgcg
     1441 agcgtgccct tcatcgggga caagctggac attttcgatc cggacatggc gcccctgtac
     1501 gactacagcg tgggcagtgg gttccgtgat ggtcggccgt gctggctctt caccgccgtg
     1561 gccagggatc aggtgggcgg tcggcctgcg gacgaggatg atacggtcat caaacgcatg
     1621 ctcacctggt tcgaccaggg gacgatgaac gtacttgcac gtgagtatcg gatgcggagc
     1681 agtggcatcc tgctcgattt cgacctcctg gcgcggatcg acgaggacac gcg
//
LOCUS       DAOQOC010000733         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51021_length_1731_cov_2.706444, whole genome shotgun sequence.
ACCESSION   DAOQOC010000733 DAOQOC010000000
VERSION     DAOQOC010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51021_length_1731_cov_2.706444"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PL106_11250"
     CDS             complement(<1..675)
                     /locus_tag="PL106_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33687.1"
                     /translation="MKHLLTLVALLVATTLLAQPMMPATTRADLEQLRGMAKASPGPD
                     ALNRAAQGRFPVSMVHGRCTVGLLGRVQGTFDPSLLTDPHVLIGSRIGNILSFRVDAH
                     HLDALDQIPGLEYAELAGRVVPQLNKAVASIHADSVQRGIWLPQTYTGRDVLIGITDW
                     GFDYSHPMFYDTTLTTSRVRAAWDQWRQAGPAPAGFSYGTELATPEALANAVSDTVNV
                     YGMATHG"
     gene            741..>1731
                     /locus_tag="PL106_11255"
     CDS             741..>1731
                     /locus_tag="PL106_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNA33688.1"
                     /translation="MSLTKSLVLLLAAPILAGGVCKPTMLAGPAKLPDIRLPEGFSIA
                     LFAENVPSVRAMTYSPDGIVYAGSNSAGAIYALPDRDRDGRADTVFTIAEGLKMPVGV
                     AWHDGDLYFSAIHLIAKLPDPDHHLADPPAPVTVFDGFPKETHHGWKFIGIGPDQKLY
                     VPVGAPCNICLSEDSVFASITRMNLDGTGLEVVAHGVRNTVGFDWDPRDSSLWFTDNG
                     RDWMGDDAPSCELDHVTAVGQHFGYPFCHQGDTLDPEFGKGKNCADYVKPVVKLGAHV
                     APLGMRFYRGDMFPEKYRGAVFIAEHGSWNRSSPVGYRVSVAFPRADGTVDHEV"
BASE COUNT          310 a          539 c          563 g          319 t
ORIGIN      
        1 gccgtgcgtc gccataccgt agacgttcac cgtgtcggac accgcattgg cgagcgcttc
       61 aggggttgcg agctcggtac cgtagctgaa gcccgccggt gccggacccg cctgccgcca
      121 ttggtcccag gccgcgcgca cgcgcgaagt ggtgagggtg gtgtcgtaaa acatcggatg
      181 gctgtagtcg aagccccagt cggtgatccc gatgagcaca tcgcggcccg tgtacgtctg
      241 tgggagccag atgccccgct gcacactgtc ggcatggatg gaggccaccg ccttgttcag
      301 ctgcggtacc acgcgaccgg ccagctctgc gtactccaat ccggggatct gatccagcgc
      361 gtccagatgg tgcgcatcca ccctgaatga aaggatgttc ccgatacggg atccgatcag
      421 cacatgggga tccgtcagca gcgatgggtc gaaggtgcct tgcacgcggc ccagcagccc
      481 tacggtacag cgcccatgca ccatggacac ggggaaccgg ccttgggccg cgcgattcag
      541 tgcgtcgggt ccgggtgaag ccttggccat gccccgcagt tgttcgagat cggcacgcgt
      601 ggtagcgggc atcattggct gtgcgaggag cgtagtggca acaagcaggg ccaccaatgt
      661 gagaagatgc ttcatgcggg caaggttgga caccacgaag taagggcatg cggagcgatc
      721 gcatccgata ccttggcccc atgtctctca ccaagtcact cgttctcctg ctggccgcgc
      781 cgatcctggc gggcggagtt tgcaaaccca ccatgctcgc tggcccggcc aaattgccgg
      841 acatccggct gcctgaagga ttcagcatcg cgctcttcgc ggagaacgta ccctcggtgc
      901 gggccatgac ctacagtccg gatgggatcg tttatgccgg ctcgaacagt gccggcgcga
      961 tctatgccct tccggacagg gaccgtgatg gccgggccga tacggtgttc accattgcgg
     1021 agggcctcaa gatgccggtg ggggtggcct ggcacgacgg cgacctctac ttctccgcca
     1081 tccacttgat cgccaagctg cccgatccgg accaccacct ggccgaccct cctgcccctg
     1141 tgaccgtgtt cgatggattt cccaaggaga cgcatcacgg gtggaaattc atcgggatcg
     1201 gtccggacca gaagctgtat gtgccggtgg gggcgccctg caacatctgc ctgagtgagg
     1261 atagcgtgtt cgccagtatc acacgtatga acctggatgg gaccgggctg gaggtcgttg
     1321 cgcacggcgt acgcaacacc gtcggctttg attgggaccc gcgtgattct tcactctggt
     1381 tcaccgacaa tggccgcgac tggatgggcg acgacgcgcc cagttgcgag ctggaccacg
     1441 tgacagccgt tggtcagcac ttcggctacc ccttctgcca ccagggcgat acgctcgacc
     1501 ccgaattcgg caagggaaag aactgcgccg attatgtgaa gccggtggtc aagctcggcg
     1561 cccacgtcgc accgctcggc atgcggttct atcgcgggga tatgttcccg gaaaagtacc
     1621 gcggtgcggt gttcatcgcc gagcacggca gttggaaccg cagcagcccg gtcggctacc
     1681 gggtgtcggt ggccttcccc agagcggatg gcaccgtgga tcacgaggtc t
//
LOCUS       DAOQOC010000734         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51049_length_1730_cov_4.419104, whole genome shotgun sequence.
ACCESSION   DAOQOC010000734 DAOQOC010000000
VERSION     DAOQOC010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51049_length_1730_cov_4.419104"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="PL106_11260"
     CDS             <1..1361
                     /locus_tag="PL106_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33689.1"
                     /translation="PDSFKYELNKVVVRCDTCTTVIGGNCNGCFLSLDQAGPFGGACT
                     TNGDHWRFEHSSGTWSVLAHAAINTTWLYDPGTGSTATVDTIMAVDVYGMPDSVKVIT
                     LSTGDTLLLSREHGVLGWPGENGTLLQIGEQTTGSGVRIPTLSEFITYQPGDVAQYTH
                     GSWGIGMSAMTGEYWTEKLTFLDRIDHADSIEFQVARITLDRVTYEVGFGQSQTVEYT
                     TSDTATWVASSAHLPFFRTIGAYPGQEIRDRTFHLEFENELIITAEHGFDPEGHHIIR
                     ATCYPFSVDPPWWNTQSLFMDADTVATGILAMDTWNTVDSFEPAVVYQEGPGLVTYQA
                     GFFETSESYRLDGAVIGGDTIGELTPDDVLLGVPMQGQQALAPFVFPNPAQDLLMVRT
                     PGTSTSMCRILDTSGKQVRMEMMDQGDLHTINVSALPAGLYVMEVRSSSRVARERFII
                     AR"
     gene            1486..>1730
                     /locus_tag="PL106_11265"
     CDS             1486..>1730
                     /locus_tag="PL106_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33690.1"
                     /translation="MIHSLPSKAITAIALSMLIALNACEPGAGSGVPDATPSHHTTQQ
                     VVVPDSIMNYLIDSAVQDFRTHRPPTAIDVRNARIGYL"
BASE COUNT          363 a          512 c          510 g          345 t
ORIGIN      
        1 gaccggatag tttcaagtat gagttgaaca aagtggtcgt gcgttgtgat acgtgtacca
       61 cggtgatcgg cgggaactgc aacggatgct tcctatcgct cgatcaagcc gggccgttcg
      121 gcggcgcgtg cactacgaac ggggaccact ggcggttcga gcattccagc ggaacgtggt
      181 cggtccttgc gcatgcggcg atcaacacca cttggctgta cgatccgggc acgggttcca
      241 cagcgacggt ggatacgatc atggctgtgg acgtgtacgg catgccggat tcagtgaaag
      301 tgatcaccct gtcgaccgga gataccctgc tgttgtcacg ggagcatggc gtactgggtt
      361 ggcccgggga gaacgggaca ttgctccaga tcggcgaaca gaccacgggt agcggtgtac
      421 gaatcccgac cctgtccgag ttcatcacct atcagcccgg cgacgtggcc cagtacacgc
      481 atggctcctg gggcatcggt atgtcggcga tgaccggtga gtactggacc gaaaagttga
      541 ccttcctgga ccgcatcgac catgcggaca gcatcgaatt ccaagtggcc cggatcacat
      601 tggaccgcgt gacgtacgag gtcggcttcg ggcagtccca aacggtggaa tacaccactt
      661 ccgacacggc gacctgggtc gcatcgagcg cccatctccc gttcttcaga accatcggtg
      721 cgtatccggg acaggagatc cgtgatcgaa cgttccatct cgaattcgag aacgagctga
      781 tcatcacggc cgaacatggt tttgatcctg aaggccatca catcatacgt gctacgtgct
      841 atccgttctc ggtggatccc ccatggtgga acacacaaag cctcttcatg gatgcggaca
      901 cggtggcaac aggcatcctg gccatggaca cgtggaacac ggtggattca ttcgagcctg
      961 cggtggtgta ccaggaaggg cccggactgg tgacgtacca ggcgggcttt tttgaaacga
     1021 gcgagtccta tcgtttggac ggtgcggtca ttggcggcga cacgatcggc gagctcaccc
     1081 cggatgacgt actgctcggt gttccaatgc aagggcaaca ggcacttgca ccgttcgtat
     1141 tcccgaatcc cgcgcaggat ctgctcatgg tacggacccc cgggacatcc acctcgatgt
     1201 gccgcatcct ggacacatcg ggaaaacagg tacggatgga gatgatggat caaggtgatc
     1261 tgcacacgat caatgtgagt gcgctacccg ctggcctgta tgtgatggag gtgagatctt
     1321 cttcacgggt ggcacgcgaa cgcttcatca tcgcgcgatg agaacactcc tgctctccat
     1381 cgtactcatg tgcggctttg cgagtcttac ccagaactgg gccctgctga acccggcttg
     1441 atagtccatg cacagcgaca aaggcagggg cacgaccttc gcagcatgat ccacagcctc
     1501 ccttcgaaag ccatcacggc catcgccttg tccatgctca ttgcactcaa cgcatgcgaa
     1561 cctggagccg gatccggggt accggatgcg accccatcgc accacacaac gcagcaggtg
     1621 gtcgttcccg attccatcat gaactacctg atcgactctg ccgtgcagga cttccggacc
     1681 catcggccgc ccacggccat cgacgttcgc aacgcccgga tcggttacct
//
LOCUS       DAOQOC010000735         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51058_length_1730_cov_3.622090, whole genome shotgun sequence.
ACCESSION   DAOQOC010000735 DAOQOC010000000
VERSION     DAOQOC010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51058_length_1730_cov_3.622090"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PL106_11270"
     CDS             complement(<1..838)
                     /locus_tag="PL106_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNA33691.1"
                     /translation="MPKVTIDNIEIDVPEGTTILQAARMIGERNKADRYVVPPAMCYY
                     TDLKTSGGYCRTCIVKVTKGSDKDPRPIPKPVASCRTAVMDGMVVANTTDEDLIASRN
                     AVVEFLLINHPLDCPICDQAGECHLQDLSYENGKESCRYQFERRTFDLIDIGDKIKLH
                     MTRCILCYRCVKVADQLTDGRVHGVINRGDVAEISTYISNVVDNDFSGNMIDVCPVGA
                     LTDRTFRFASRVWFTKPMDAHRDCDKCSGKVVVWYKGEEILRVTGRKDQWGEVEEFIC
                     NTC"
     gene            complement(933..>1730)
                     /locus_tag="PL106_11275"
     CDS             complement(933..>1730)
                     /locus_tag="PL106_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33692.1"
                     /translation="IWRVKLEGGDNKQLRMGVDIAAGITRKLSLKAGLGGWKGMLVWL
                     PEMMDPQAANKLLKILEEPEPKTAFLLVGHENERILPTILSRTQLVKVPAMNAGEIGD
                     ALSERYPDLAPEEKGRAAARSEGDLLEASAMVEKSEVDLFVFFRDWLRASYGDRIAEV
                     VEIAETFSKMGREKQKSFVRYALYIIRQSVLEWQAVPQLVRANDEEMEFVLKFSRMLN
                     AHNIEGIRQELEAAHGHLERNANTKVLLLDLSYRVGELLRTKVGSPV"
BASE COUNT          280 a          588 c          500 g          362 t
ORIGIN      
        1 ggcaggtgtt gcagatgaat tcctccacct cgccccactg gtccttgcgg ccggtgacgc
       61 gcaggatctc ctcgcctttg taccacacca ccaccttgcc gctgcatttg tcgcagtcgc
      121 ggtgggcgtc catcggcttg gtgaaccaca cgcggcttgc gaaacggaac gtgcggtcgg
      181 tgagcgcgcc caccgggcac acatcgatca tgttcccgct gaagtcgttg tccaccacgt
      241 tgctgatgta cgtgctgatc tcggccacat ccccgcgatt gatcaccccg tgtacacgcc
      301 cgtcggtgag ctggtcggcc accttcacgc agcggtagca caggatgcac cgcgtcatgt
      361 ggagcttgat cttgtccccg atgtcgatca ggtcgaaggt gcggcgctcg aactggtagc
      421 ggcacgattc cttgccgttc tcgtaactga ggtcctgcag gtggcactcg ccggcctggt
      481 cgcagatcgg gcaatccagc gggtggttga tcagcaggaa ctccaccacg gcattgcgcg
      541 aggcgatcag gtcctcatcg gtggtgttgg ccaccaccat cccgtccatc acggcagtgc
      601 ggcaactggc caccggcttg ggtatgggcc gggggtcctt gtcgctgcct ttggtcacct
      661 tcacgatgca cgtgcgacag tatccgccac tggtcttcag atcggtgtag tagcacatgg
      721 ccggcggcac cacatagcgg tcggccttgt tgcgctcgcc gatcatgcgc gcggcctgca
      781 ggatcgtggt cccttccggt acgtcgatct cgatgttgtc gatggtgacc ttgggcatgc
      841 gtgctcgccg aagaagtgcg gaagtgggtt ccggcgcgct gcaaagtaaa gaggcgggaa
      901 ccaacggcac acgcccgacc agaagtggga tctcagaccg ggctgcccac cttcgtccgc
      961 agcaactccc ctaccctgta actcaggtcc agcaacagca ctttggtatt ggcgttgcgc
     1021 tccaggtggc cgtgcgccgc ttccagctcc tgccgaatgc cctcgatgtt gtgcgcgttc
     1081 agcatccggc tgaacttcaa cacgaattcc atctcttcgt cgttggcccg cacgagttgc
     1141 ggcacggcct gccattccag gacgctctgc cggatgatgt acagcgcgta gcgcacgaag
     1201 gatttctgtt tctcccggcc cattttgctg aaggtctccg cgatctccac tacctcggcg
     1261 atccggtcgc catagctcgc ccgcaaccag tcgcggaaga acacgaagag gtcgacctca
     1321 ctcttctcaa ccatggccga ggcttccagc aggtcgccct cgctgcgggc cgctgctctc
     1381 cccttctcct cgggagcaag gtccgggtag cgttcgctga gggcgtcccc gatctcccct
     1441 gcattcatgg cgggcacctt caccaactgc gtgcgactga ggatggtggg caggatgcgc
     1501 tcgttctcgt ggcccaccag caggaaggcc gtcttcggct cgggctcttc caggatcttc
     1561 agcagcttgt tcgccgcttg cggatccatc atctccggca accagaccag catccccttc
     1621 cagccgccca ggcccgcctt caagctcaac ttgcgcgtga tccccgccgc gatatccacg
     1681 cccatgcgca gttgcttgtt gtcccccccc tccaacttca cccgccagat
//
LOCUS       DAOQOC010000736         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51062_length_1730_cov_3.188060, whole genome shotgun sequence.
ACCESSION   DAOQOC010000736 DAOQOC010000000
VERSION     DAOQOC010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51062_length_1730_cov_3.188060"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..658
                     /locus_tag="PL106_11280"
     CDS             281..658
                     /locus_tag="PL106_11280"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNA33693.1"
                     /translation="MDPYHLPATDKTPTVRFIPAEGLLEIAGCSIHENADRFFRPLLE
                     RVAAYAREPMPETLVRITLSYFNSSSAKYMLDLLKLLDDVHAGGLGKVAVEWYFAQGD
                     LDMQEAGQDYRGLLDMPVRLVEQ"
     gene            complement(815..>1730)
                     /locus_tag="PL106_11285"
     CDS             complement(815..>1730)
                     /locus_tag="PL106_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33694.1"
                     /translation="ASDVFSMDVIQPRSGAIPIDAPMPSGVVYKVQIGAFRQDIPAQL
                     FSDMTPVTGESVANGLTRYTAGMFTTPEGAVKAAETVRQRGYRDAFVVAYQDGRRVSL
                     GQAMRAGRPTTAQVTGTISANTTASRPGTTVRIEATGTSGIVGAQGGTVAMTDSARAA
                     EQAVLANYPASADEVLAQFAPTADATAYYNVPGAAPARQVETVKGLFFTVQVGVYSKP
                     TALDKLFNITPLNSERTENAKIRYTTGVYVDMDKARSRKDEAVGLGVKDAFITAYLNG
                     KRIPMRDARALINKFGTAVFADPGVITR"
BASE COUNT          328 a          533 c          543 g          326 t
ORIGIN      
        1 aaatcggtgg gttgctcgta cacatgcaca acatcgatgc aggcgcccgt gagccgggcg
       61 atcttggcag ccacacggag ggcatcggtg gcgcaatccg agaagtcgta cggaacaagg
      121 atgttcttga tggccatgtg gtggacgccc gccaagtagc ggacgcggag ttgggtggtc
      181 aggtccgatc tcagggctga aattaacgaa cggccggtca ccgaccaagc attcgtcata
      241 attaatgccg gaggaatcct cctactttgc ccccacgacc atggacccgt accacctccc
      301 tgctacggac aagaccccca ccgtgcgctt cattccggcc gagggcctgc tggagatcgc
      361 ggggtgcagc atccacgaga atgccgaccg gtttttccga ccgttgctcg aacgggtagc
      421 ggcatacgcc cgcgaaccga tgccggaaac gctcgtgagg atcaccctga gttatttcaa
      481 cagcagcagc gccaagtaca tgctcgatct gctgaagcta ttggacgacg tccacgcagg
      541 cggcttgggc aaagtggccg tggaatggta cttcgcccaa ggggacctgg acatgcagga
      601 ggccgggcag gattatcgcg gcctgctcga tatgccggtt cgtctggttg agcaataagc
      661 gcagcacacg ggaaggggtg tcggcgtgac caaacaagca ttcggactcc tgccctactt
      721 cgttcactga gccaccgtcc gttggtgctg cgggtcttgc acaccaccca cccgatgttc
      781 caacacggcg cacagtggaa ggtgcagggc accttcaacg cgtgatcacg cccggatcgg
      841 cgaagacggc cgtaccgaac ttgttgatca gggcacgcgc atcgcgcatg gggatccgtt
      901 taccgttgag gtaggcggtg atgaaagcat ccttcacacc cagtcccacg gcctcatcct
      961 tgcgcgaacg ggccttgtcc atgtccacat acacgccggt ggtgtagcgg atcttggcgt
     1021 tctcggtgcg ctcgctgttg agcggagtga tattgaagag cttgtccagt gcggtaggct
     1081 tgctgtacac gcccacctgc accgtgaaga agagtccctt cacggtttcc acctgtcgcg
     1141 cgggtgcggc acccggaaca ttgtagtagg ccgtcgcatc cgccgttggc gcgaattgcg
     1201 ccagtacctc atcggcggaa gccggatagt tggccagcac ggcctgctca gccgcacgtg
     1261 cgctgtcggt catggccacg gtcccaccct gtgcgccgac gatgcccgat gtgcccgtcg
     1321 cctcgatccg gaccgttgtt cccggacggc tcgccgtggt attcgccgag atagtcccgg
     1381 tgacctgtgc ggtggtggga cgcccagcac gcatggcttg accgagggag accctgcgcc
     1441 catcctgata ggccaccaca aaggcatcgc ggtagccgcg ttggcgaacg gtctccgcgg
     1501 ccttgaccgc cccctcgggc gtggtgaaca tgcccgccgt gtagcgcgtg aggccgttgg
     1561 caacgctttc tccggtcacc ggggtcatgt cgctgaacag ctgtgcggga atgtcctgcc
     1621 tgaacgcacc gatctgcact ttgtacacca cacccgaagg catgggcgca tcaatgggga
     1681 tggcaccgga gcgcggttgg atcacgtcca tggagaatac gtccgaagcg
//
LOCUS       DAOQOC010000737         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51102_length_1729_cov_4.457587, whole genome shotgun sequence.
ACCESSION   DAOQOC010000737 DAOQOC010000000
VERSION     DAOQOC010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51102_length_1729_cov_4.457587"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PL106_11290"
     CDS             <1..1316
                     /locus_tag="PL106_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNA33695.1"
                     /translation="PYFSAMRFQHVLLLLGLPALLLPACKKDEGGPPPSVRITTPGDG
                     FDLNVPDTLHVEADVSGDDGVDMVSFMISDANGIAIMAPVIVVPGSNPAHLSIDLPVT
                     SEQLTGGEYTLTVRAVSGELSTKAYVHFDIVPAPLRTRQVLVLVRPDPGTVAIHVIDS
                     TNTVSMASQLSMDLAGAVVSSTTQRYMLAGSTQGPLMALAPSAAQTLWEIPNNGGGAT
                     WFTALDLCTDGRCYAGTADSRLRGYNTLTGALERTLQIGGGRRVRHSCIVGDRIVTAE
                     SDALGAAWMTASYQSMSGVPIAEHPLALKVVGMYLRDDEQVLFFGDQNGMGTVRDHNV
                     EGGGGWNPYQWNAAITAVERVDAGTFIVALDNGSVERFTYPSSGSVPIAQVDGVNDLA
                     YEPVSGLVYAGTGSDVLAINPQTGQVVMSIAIGAPVAYVLPLLNR"
     gene            complement(1419..>1729)
                     /locus_tag="PL106_11295"
     CDS             complement(1419..>1729)
                     /locus_tag="PL106_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNA33696.1"
                     /translation="NTWYSVISPESCSSILWRSWDFKEKAAEALKLTSQDMLKSKLID
                     GIIPEPVGGAHADVDKATSLVKAEVKKHLDKLVKIDPDKLVAKRVEKFCSMGVTVKVK
                     "
BASE COUNT          310 a          539 c          553 g          327 t
ORIGIN      
        1 gaccttactt ttcggcaatg cgttttcaac acgtgctgtt gctgctgggc ctcccggctc
       61 ttctccttcc ggcatgcaag aaagacgagg gtgggccacc gccatcggtg cggatcacca
      121 cccccggcga cgggttcgac ctcaatgtgc ccgatacgct gcacgtggaa gcggacgtga
      181 gcggggacga tggggtggac atggtttcct tcatgatcag cgatgcgaac gggatcgcga
      241 tcatggcgcc cgtgatcgtg gtgcccggat cgaacccggc ccatttgagc atcgatctgc
      301 cggtcacctc agaacaactg accggcggtg agtacacatt gaccgtacga gccgtttccg
      361 gggagctcag tacaaaggcc tatgtacact tcgatatcgt acccgccccg ttgcgaacaa
      421 ggcaggtgct ggtcctcgtg cgtcccgatc caggaaccgt agcgatccac gtgatcgaca
      481 gcaccaacac ggtctcgatg gccagccagt tgagcatgga cctggcgggc gccgtggtca
      541 gcagcaccac acagcgctac atgctggccg gtagcaccca agggccgttg atggccctgg
      601 caccgtcggc cgcgcagacg ctctgggaga tcccgaacaa cggaggtggg gccacgtggt
      661 tcaccgcact ggacctgtgt accgacgggc gttgttacgc gggcacggcg gacagccggt
      721 tgcgcggtta caacaccctc acgggggcgc tggagcgcac cctgcagatc gggggtgggc
      781 gccgggtgcg gcactcatgc atcgtgggcg atcggatcgt gaccgccgag tcggatgccc
      841 tcggcgccgc atggatgacc gcaagctacc agtccatgtc gggggtgccc attgcggaac
      901 atccgctcgc gctgaaggtg gtgggcatgt acctccgcga cgatgaacag gtattgttct
      961 tcggcgacca gaacggcatg ggcaccgtgc gcgaccacaa cgttgaagga ggcggcggtt
     1021 ggaaccccta ccaatggaat gcggccatca ccgccgtgga acgtgtggat gcgggcacct
     1081 tcatcgtagc cctcgacaac gggtccgtgg agcgttttac atacccttcc tccggttctg
     1141 tccccattgc gcaggtggat ggcgtgaacg acctggccta cgaacccgtg agtggtctgg
     1201 tgtacgcggg aaccggtagc gatgtgctgg ccatcaaccc gcagaccggt caagtggtga
     1261 tgtccatcgc gatcggcgcc ccggtggcgt acgtgctgcc gttgctgaac cgctgagcgt
     1321 acgaacggga cgcacgaccc gctcctgcca gccccccttg cacgggccat cacccgtggg
     1381 aacggggtgc ataacgtgcg gtcggcggga cggcagacct atttgacctt caccgttacc
     1441 cccatggagc agaacttctc cacgcgcttg gccaccagtt tatcgggatc gatcttcacc
     1501 agcttgtcca ggtgcttctt cacctcggcc ttcaccagcg acgtggcctt gtccacatcg
     1561 gcatgggcgc cgcccacggg ttcggggatg atgccatcga tcagcttgct cttcagcatg
     1621 tcctgcgagg tgagtttgag cgcctcggcc gccttctcct tgaaatccca gctgcgccag
     1681 aggatggagg agcaggattc gggggagatc acactgtacc aggtgttct
//
LOCUS       DAOQOC010000738         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51330_length_1726_cov_2.916816, whole genome shotgun sequence.
ACCESSION   DAOQOC010000738 DAOQOC010000000
VERSION     DAOQOC010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51330_length_1726_cov_2.916816"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PL106_11300"
     CDS             <1..1164
                     /locus_tag="PL106_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNA33697.1"
                     /translation="VWDYVINFSHLLVPFPNWKVLQPNIQLFRDNGVPMVFEQGLSSP
                     GGEMPEFRCYLLAKLLWNPDVNIDSVRTHFMNAYYGDAGAYIDKYTRLLEEELDKSGK
                     ALTLYEHPQAHKEGYLSPANLAKYKMLFNSAEAAVMDEDPIYQQRVEAARLPIMYAEL
                     EVARAMPGTPNGLFEMDPAGPRRAKPDYIALLDTFTVRAKRHGNLLLHETRLTPDEYR
                     AQFSLYLKEGAVTHLAARKAIQFTTPPDPKYTGGSPNALVDGFRASTNYQFAWQGWWG
                     KDLVATIDLGKPTQVASVEMGFLEDQASWIFLPSIVSVETSTDGQHWTSFGKRDVELK
                     KQVGAPTIQTVRIEGTGTMQRFVRITAKNLGALPAFHGSKGECWLFCDEIVVR"
     gene            complement(1265..>1726)
                     /locus_tag="PL106_11305"
     CDS             complement(1265..>1726)
                     /locus_tag="PL106_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNA33698.1"
                     /translation="RQVASPPPARQYGRDPDHGMTDGLLGTTTYHDGLWQGWWGVDPT
                     ITIDLDSARIINEVRVRCLQQVGVWIVLPRTVEFYISDDGVKWQAFSTATHTIPVEGA
                     GPLVHDFITHPQTAVRARYVRAVLRNSGKLPAWHLGAGGDSWVFADEVIVR"
BASE COUNT          346 a          545 c          531 g          304 t
ORIGIN      
        1 gtgtgggact acgtgatcaa cttctcgcac ctgctcgtgc ccttccccaa ctggaaggtg
       61 ttgcaaccca acatccagct cttccgcgac aacggcgttc cgatggtctt cgagcaaggg
      121 ctctcttcgc ccggtggcga gatgccggag ttccgttgct acctgctggc gaagctgctc
      181 tggaaccctg atgtgaacat cgactccgtg cggacgcact tcatgaacgc gtactatggt
      241 gatgccggtg cgtacatcga caagtacacg cgtttgctgg aggaagagct tgacaagtcg
      301 ggcaaggcgc tcacgctcta cgaacatcca caggcacaca aagaaggcta cctcagcccg
      361 gcgaacctcg ccaagtacaa gatgctcttc aacagtgccg aggctgcggt gatggacgag
      421 gacccgatct atcagcaacg tgtggaggcc gcacggcttc ccatcatgta cgccgaactg
      481 gaggtggcgc gggccatgcc tggcacgcct aacgggctct tcgagatgga ccccgccggg
      541 ccgcgacgtg ccaaacccga ctacatcgca ctgctggaca cattcacggt gcgcgccaag
      601 cgacacggca acctgctgct gcatgaaaca cgactcaccc ccgatgagta tcgggcgcag
      661 ttcagtctct atttgaaaga gggcgccgtg acccatctcg ccgctcggaa ggcgatccag
      721 ttcaccacac cacccgatcc caagtatacc ggcggttccc ccaacgcatt ggtggacggc
      781 ttccgcgcca gcaccaacta ccagttcgca tggcagggct ggtggggcaa ggatctggtg
      841 gcgacgatcg acctgggaaa gccgacccag gttgcgtcgg ttgaaatggg tttcctggag
      901 gaccaggcca gctggatctt cctgccttcg atcgtttcgg tggaaacgtc aacggacggc
      961 caacactgga cctccttcgg aaagcgggat gtggaattga agaagcaggt gggagcgccg
     1021 accatccaga ccgtacggat cgaaggcact ggcacgatgc agcgtttcgt gcgcatcacg
     1081 gcgaagaacc tcggcgcgct gccagccttc cacggcagca agggcgagtg ctggttgttc
     1141 tgcgatgaga tcgtggtgag gtagcccatt caccacagag gcacagagca cacccctcac
     1201 cccaacccct ctcccaagga gaggggcacc tctgtgcctc tgtggtgaag cattgacgcg
     1261 gggatcaccg cacgatcacc tcatcggcga agacccacga atcgccgcca gcgcccaggt
     1321 gccaggcggg cagcttgccg ctgttgcgca gcacggcccg cacgtagcgc gcacgcaccg
     1381 ccgtctgcgg atgcgtgatg aagtcatgca ccagcggtcc ggcaccttcc acggggatgg
     1441 tgtgcgttgc ggtggagaac gcctgccatt tcacgccatc gtcggagatg tagaactcca
     1501 cggtgcgcgg cagcacgatc cacacgccca cttgttgcag gcaacgaaca cgcacttcgt
     1561 tgataatgcg cgcgctgtcc agatcgatgg tgatggtggg atccacgccc caccagccct
     1621 gccacaggcc atcgtggtag gtggtggtgc ccagcaggcc gtcggtcatg ccgtgatcgg
     1681 ggtcgcggcc gtattgccgt gcgggcggtg gcgaggcgac ctgtcg
//
LOCUS       DAOQOC010000739         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51337_length_1726_cov_2.704967, whole genome shotgun sequence.
ACCESSION   DAOQOC010000739 DAOQOC010000000
VERSION     DAOQOC010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51337_length_1726_cov_2.704967"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PL106_11310"
     CDS             <1..884
                     /locus_tag="PL106_11310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNA33699.1"
                     /translation="CRVVFGEADGLPALVVDKFNDVLVIQTLALGMDRFKPLIVEALR
                     EVTGVSKLYERNDVPVREKEGLEQRTGFLSEPFDTGLTIIENDLALRVDIAHGQKTGH
                     FLDQRQNHAAIARISKGARVLDCFTHTGGFALHAAMYGARDVLGLDISEGAVALAKRN
                     AEANGLADRCRFITANVFDFLTEASKRGDQWDVVVLDPPSFAKSRGALEGAVRGYKEI
                     NLRAMKCLPKGGFLVTCSCSQHMTPDLFRAMVADAARDAHRTLRELYSGTQPPDHPIC
                     WSIPETLYLKCLVLQVE"
     gene            1000..>1726
                     /locus_tag="PL106_11315"
     CDS             1000..>1726
                     /locus_tag="PL106_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HNA33700.1"
                     /translation="MGRSDSFHDALVKAPMLRLSLPFIGGLVLARAFQPAWPWPGIAA
                     TVLLVLWFVQSGSVRRFGQRWQRGALLYPGLLLFGMGWQVLRQPERMDTLARQRMTTV
                     VWRMRVTEAISTGRSTMRLWARVDAAADSAGVRAAHGGVLLTLVSDSGLALPGTGDEL
                     LVHARIDTIDRVPDPGGFDGRAWAASFGIRHSCFAGRGQWRVHRSATGFASFFEGARR
                     RVSAWLEASSLSAQSAAVPGGPDT"
BASE COUNT          281 a          538 c          584 g          323 t
ORIGIN      
        1 gttgtcgcgt ggtgttcggc gaggccgatg gtctcccggc actcgtagtg gacaagttca
       61 acgacgtgct cgtgatccag accctcgccc tgggcatgga ccgtttcaag ccgctcatcg
      121 tggaagcgct gcgcgaagtg accggtgtga gcaagctgta cgaacgcaac gacgtgcccg
      181 tgcgcgaaaa ggaagggctg gagcagcgca cgggattcct gagcgagccg ttcgatacgg
      241 ggctcacgat catcgagaac gacctggccc tgcgggtcga tatcgcccac gggcagaaga
      301 ccggccattt cctggatcag cggcagaacc acgccgccat cgcgcgcatc agcaagggcg
      361 cacgcgtgct ggactgcttc acgcataccg gagggttcgc actgcacgcg gcgatgtacg
      421 gcgctcgtga tgtgctgggg ctcgacatca gcgagggcgc cgtggcgctg gccaagcgca
      481 acgccgaagc gaacgggctg gccgaccgct gccgcttcat caccgccaac gtgttcgact
      541 tcctcaccga ggcaagcaaa cgtggcgacc agtgggacgt ggtggtgctc gatccgccgt
      601 cattcgccaa gagccggggc gccctggagg gagctgttcg cggctacaag gagatcaatc
      661 tgcgggccat gaagtgcctg cccaaaggcg gctttctcgt cacgtgctcc tgctcgcagc
      721 acatgacgcc ggacctgttc cgtgccatgg tggccgatgc tgcccgcgat gcgcaccgca
      781 cactgcgcga actgtattcc ggcacccagc caccggacca cccgatctgc tggtccatac
      841 ctgaaacatt gtatctgaaa tgcctcgtgc tgcaggttga gtgagggcgg ccatggagcg
      901 gtgacccttc gtcaactatc ttcatcgcgt ccctcggcgg tcccgcttca agcactccgc
      961 cattcgagga cagggtgatc ttctggaacc gtgaaacgga tggggcggag cgactccttc
     1021 cacgatgcgc tggtcaaggc gccgatgctt cggctctcct tgccgttcat tggtgggctc
     1081 gtactggcgc gcgctttcca accagcatgg ccgtggcccg ggatagcagc cacggtcctt
     1141 ttggtactct ggttcgtaca gtccggcagc gtacgcaggt tcggccaacg atggcagcgt
     1201 ggcgcgctgc tttatccggg actcttgctg ttcggcatgg gctggcaggt attgcggcag
     1261 ccggaacgga tggacacact cgcacggcaa cgcatgacca ccgtggtctg gcgcatgcgg
     1321 gtgaccgagg cgatctcgac cggacggtcc acgatgcggc tctgggcccg ggtggatgcc
     1381 gcggcggaca gtgccggggt gcgcgctgca cacggcggcg tattgctcac cctggtatcg
     1441 gacagtggcc tcgccttgcc gggcaccggc gatgaactgc tcgtgcatgc ccggatcgat
     1501 accattgatc gcgtacctga tcccggtggg ttcgatggtc gtgcatgggc cgcatcgttc
     1561 ggtatccgtc acagttgctt tgcagggcgg ggccagtgga gggtgcatcg gtcggccact
     1621 ggtttcgcgt cgttcttcga aggtgcgcgc cgccgtgtat cggcctggtt ggaggcctcg
     1681 tcgctcagcg cgcagagcgc cgcggttccg ggcgggccgg acacgg
//
LOCUS       DAOQOC010000740         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51389_length_1725_cov_3.125749, whole genome shotgun sequence.
ACCESSION   DAOQOC010000740 DAOQOC010000000
VERSION     DAOQOC010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51389_length_1725_cov_3.125749"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..583
                     /locus_tag="PL106_11320"
     CDS             95..583
                     /locus_tag="PL106_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HNA33701.1"
                     /translation="MKGFHVMDDGSAVHLLDHVRELIAAQPDVEVLVGSDSQNRSGST
                     IYTTAVVLRYRRNGAHVLYCREDRERVRDLWTRLWGEVERSLDVAQALVEGGVPVRSI
                     DMDLNSDPRFGSNKLHNAAVGHVRALGYVAHTKPEMLIATWAANVLCQRWGRKHEAPT
                     GL"
     gene            complement(744..>1725)
                     /locus_tag="PL106_11325"
     CDS             complement(744..>1725)
                     /locus_tag="PL106_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNA33702.1"
                     /translation="HGPRRDRGTQGDAEGRAAGEAGDAAPSTPIGVNLPNADWIRAEH
                     GSKSVSLGNITDAYEQSGGKSALEVFCNDPEEVERAIAQGGLAGELHTALHEVIGHAS
                     GQLEPGVEGTDVTLKNYASTLEEGRADLVALYFLMDPKLVELGVMPSLETGKAEYDSY
                     LRNGLLAQLRRIKPGNSIEEAHMRNRQWVSAWVVERGLKNGAVARNERNGDIYYDILD
                     YEKMRSLFGELLREVQRIKSQGDYEAGKALVETYGVKVDPKVHANVLARAARITVPPY
                     AGFIQPRLVPVMNASGQITDVRVEYPGNFLEQMLEYDRDHSFLPEQILVR"
BASE COUNT          290 a          559 c          527 g          349 t
ORIGIN      
        1 gacgacgtgt ggcgaatcgg gatcggcgag gaactcgtcc atccactgat cggtagcgga
       61 acacaatacg cttcccgtta ccttgtggca ttcgatgaag ggttttcacg tgatggacga
      121 cggttcggcc gtgcatctgc tggaccatgt ccgggagctc atcgctgcgc aacccgatgt
      181 ggaggtgctc gtgggcagcg acagccagaa ccggagcggc agcaccattt acaccaccgc
      241 cgtggtcctt cgctatcgac gcaacggggc ccatgtgctc tattgccgcg aggatcgcga
      301 acgggtccgc gatctgtgga cgcgcctctg gggcgaggtg gaacgcagct tggacgtggc
      361 gcaagcactg gttgagggtg gagtgcccgt gcggagcatc gacatggacc tgaacagcga
      421 cccccgcttc ggatcgaaca aactgcacaa tgccgccgtg ggacatgtac gcgctctcgg
      481 ctatgtggca cacaccaaac cggagatgct cattgccact tgggcagcga atgtgctgtg
      541 ccagcggtgg ggccgcaaac acgaagcccc caccggtctg tgacctaggc aggggcgacg
      601 tgactcttca ggttcgacct atgaactact tggacagggg ttggaacgct cgaccggggg
      661 atgcgggttg cgcccaagga tcggctattc gccaagtggt ggtatttgcc cgatgaaccg
      721 cttccggatc ccgccgaaca ctcctaccgg accaggatct gttccgggag gaagctgtgg
      781 tcgcggtcgt actccagcat ctgttccagg aagttcccgg gatattccac acgcacatcg
      841 gtgatctggc cggatgcgtt catcaccggt accaagcgcg gttggatgaa tcccgcgtac
      901 gggggcactg tgatgcgggc cgcgcgagcc agcacattgg catgcacctt cgggtccacc
      961 ttcacaccgt aggtctccac cagtgccttg ccggcctcgt agtcgccctg gctcttgatc
     1021 cgttgcacct cgcgcagcaa ctcgccgaag agcgagcgca tcttctcata atccaggatg
     1081 tcgtaataga tgtcgccgtt gcgttcgttg cgcgccacag caccattctt caggccacgt
     1141 tccaccaccc aagcgctcac ccattgccgg ttgcgcatgt gggcctcttc gatgctgttg
     1201 cccggcttga tgcgtcggag ttgcgcgagc agcccgttgc gcaggtacga atcgtactcg
     1261 gccttgcccg tctccaggct cggcatcaca ccgagttcca ccaacttggg atccatcagg
     1321 aagtacaggg ccaccaggtc ggcacgaccc tcttccaagg tgctcgcgta gttcttgagt
     1381 gtaacgtccg tgccttcgac gcccggctcg agttgcccgc tggcatggcc gatcacttcg
     1441 tgcagtgccg tgtgcagctc gcccgcgagc ccgccctgcg ctatggcccg ttccacctct
     1501 tcggggtcgt tgcagaagac ctcgagcgcg ctcttccctc cgctttgctc gtatgcatcg
     1561 gtgatattcc ccaagctcac gctcttgctg ccgtgctccg ccctgatcca atcggcgttg
     1621 ggcaggttca caccgatggg cgtgctgggc gccgcatcgc ccgcttcacc ggcggcgcgc
     1681 ccctcggcgt cgccctgcgt ccctcgatcg cgccggggtc catgg
//
LOCUS       DAOQOC010000741         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51566_length_1722_cov_3.082184, whole genome shotgun sequence.
ACCESSION   DAOQOC010000741 DAOQOC010000000
VERSION     DAOQOC010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51566_length_1722_cov_3.082184"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PL106_11330"
     CDS             <1..587
                     /locus_tag="PL106_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33703.1"
                     /translation="SLMGTGLDPATTDVFIASVLDAAPTYELQLDGQPWFAHVTCDVS
                     DTSNIYAIDFWLVRERSGMRRKWSILASSFDYEKESPLLFIGSKNHEVNFSQLLGKDI
                     LMEHGVAPYLGPPGVRHLFALQEGLKSMVWTIDAVTQVRMHLLQIPGWFAEVEYRPPP
                     ARDGWVTSGWNIVELRPMSDADKTMYIWHEILGL"
     gene            568..>1722
                     /locus_tag="PL106_11335"
     CDS             568..>1722
                     /locus_tag="PL106_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33704.1"
                     /translation="MRSSAFERLMPAWERPGRMLLLMLLGSVGIACIGAATPDSLTIK
                     DKVSINKRAIEKVDLYVNVLNAITRLDPGVPEDMDQLMEDVRPQTDRANDDRIFSGPN
                     VIIEDNIVPSNKAGSVGMDREVAVYISDFFVNFRSDGSPEPVIMRLLDQGEPKRSPDG
                     RVITQMLYEVTWKGTNRNDPVPYAASKRVVLFEARYKEGRGWSAYIGAENFYDEAQGF
                     VSFQLDRQVKDAKASGSQLSGELLEYEEAAKRAAAEAERERAERKRSFDQAIANGDEQ
                     LKKGEFDTARDLYNAAKTIDPASFVTVIIKMREVEKARLAFEQKQRKDFDTEVKKAEQ
                     LYGIKEYQRSLESFQAADRIIPGDPNVAWRMDSLNARIRAKADRESAYNTG"
BASE COUNT          377 a          463 c          572 g          310 t
ORIGIN      
        1 gatcgttgat gggcaccggt ctcgatccgg ccaccacgga tgtcttcatc gcatcggtgt
       61 tggatgccgc cccgacctat gagttgcaat tggatggcca gccctggttc gcccatgtca
      121 cctgcgatgt gagcgacacc tcgaacattt acgcgatcga cttctggctg gtgcgtgagc
      181 ggagcggcat gcgcaggaaa tggtccatcc tggcatcatc gttcgactac gagaaggaga
      241 gtcccttgct gttcatcggc tcgaagaacc acgaggttaa tttttcccag ttgctgggga
      301 aggatatcct gatggagcac ggggtggcgc cgtacctggg ccctccgggg gtccgacatt
      361 tgttcgcctt gcaggaaggg ctcaagagca tggtgtggac gatcgacgcg gtgacccagg
      421 tgcgcatgca cctgctccag atccccggct ggttcgccga ggtggagtac cgaccgcctc
      481 ccgcacgcga tggatgggtc acatcgggtt ggaacatcgt ggaactgcgc cccatgagcg
      541 atgcggacaa aaccatgtac atctggcatg agatcctcgg cctttgagcg gctgatgccg
      601 gcatgggagc gtccggggcg gatgctcctg ttgatgctcc tgggatcggt gggcatcgca
      661 tgtatcgggg ccgcgacccc ggacagcctt accatcaagg acaaggtgtc catcaacaaa
      721 cgcgcgatcg agaaggtgga cctgtacgtg aatgtcctca acgcgatcac ccggctggac
      781 cccggtgtgc ccgaggatat ggaccaattg atggaggatg tgcggccaca gaccgatcgg
      841 gccaacgatg atcgcatctt ctccggcccc aatgtgatca tcgaggacaa catcgttcca
      901 tcgaacaaag cggggtccgt tggtatggat cgggaggtgg ccgtgtacat cagcgatttc
      961 ttcgtgaatt tccgcagcga tgggagtccc gagccggtga tcatgcgctt gctggaccaa
     1021 ggggagccga agaggtcccc ggatggccgg gtgatcaccc agatgctgta cgaggtgacc
     1081 tggaaaggga ccaacaggaa cgatccggta ccgtatgcag cgagcaagcg cgtggtcctg
     1141 ttcgaggcac ggtacaaaga ggggcgcggc tggagcgcat acatcggagc ggagaatttt
     1201 tacgacgagg cacagggctt cgtgagcttc caactggaca ggcaggtgaa ggacgccaag
     1261 gccagcggga gccagttgtc cggtgagctg ctggagtatg aggaagcggc caaacgtgct
     1321 gcggccgagg ctgagcggga gcgcgctgaa cgaaagcgtt ccttcgatca agcgatcgcg
     1381 aacggggatg aacaactcaa gaaaggggag ttcgataccg cacgggacct gtacaacgcg
     1441 gccaagacga tcgaccccgc ctccttcgtg acggtgatca tcaaaatgag ggaggtggag
     1501 aaggcgcggc tggcgttcga acagaagcaa cggaaggatt tcgatacgga agtgaagaag
     1561 gccgaacaac tgtacgggat caaggagtac cagcgatcgc tggagagttt ccaagcggcc
     1621 gacaggatca ttccgggcga ccccaacgtg gcctggcgca tggattcatt gaacgcccgt
     1681 atccgggcca aggccgatcg ggagagcgca tacaacaccg gc
//
LOCUS       DAOQOC010000742         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51616_length_1721_cov_3.522809, whole genome shotgun sequence.
ACCESSION   DAOQOC010000742 DAOQOC010000000
VERSION     DAOQOC010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51616_length_1721_cov_3.522809"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..1644)
                     /locus_tag="PL106_11340"
     CDS             complement(334..1644)
                     /locus_tag="PL106_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNA33705.1"
                     /translation="MRTLYRLLGITALWVTMMDPASAQGPAPALVGYWHNWDDASAPY
                     LELDQIDPRYNVIEVAFAEPAPGTTYNMQFTPSGIPQATFIDQVAAHQASGHQVLISI
                     GGANATVHLGSDAERDQFVTSMMNILSTYGFDGIDIDLEGASMSITGGTIAAPADEPV
                     IRLIEALNGIMSQFRTMTGEKLFLTIAPETAYVQGGQSAFGGIWGAYLPVIHALRDSL
                     DILQVQLYNSGSMFGLDGGIYTQGTADFIVSQTEAVIQGFSTGGGEFPGLPAEKVAVA
                     LPACSAAAGGGYTDTATVRLAMEYLLGQGPQPGAYTLQVPGGYPALRGLMTWSINWDA
                     VPTCDGADGFAENFERIFGDTPTGIVDTSRPGSRVYYDPDTDLLWCTACGAVVLYDQL
                     GRRILFDRRNSSGTTLDLSSLNDGVYLVVEDVQGHAKAHRFVKY"
BASE COUNT          331 a          549 c          511 g          330 t
ORIGIN      
        1 tagccgctcc ggcgatgcga ggagcactac aacaggccgg tatcctttcc gaatacacga
       61 tcctcagcac gttcagaccg cgggaagtgc gctgagcacg agagtaaggc gcttcggcgc
      121 gctcagcgtg acagtgggga caagagtgtc caggagcaca gttctgtcac tcccaatgaa
      181 ctgtcacgcg gagcctgccg actgcgccta tgcatagccg ctccggcgat gcgaggagca
      241 ctacaacagg ccggtatcgt tcctgagtac ccgatcccca gcacattcag atcggtctga
      301 acatttcttt gcgcggatcg gtccgggcaa tgctcagtac ttcacgaacc gatgcgcctt
      361 cgcatggcct tgaacatcct cgaccaccag ataaaccccg tcattcaaac tgctcaggtc
      421 gagcgtagta cctgaactgt tccgccgatc gaagaggatc cggcgcccca actggtcata
      481 caggaccacc gcgccgcacg ccgtacacca gagcagatcg gtgtcgggat cgtagtacac
      541 gcgtgatccc gggcggctgg tatcgacgat ccccgtgggt gtatccccga aaatgcgctc
      601 gaaattctcg gcgaagccat cagccccatc gcaagtcgga accgcgtccc aattgatgct
      661 ccaggtcatc aggccgcgca acgcgggata gccgccgggg acctgcaacg tgtacgcgcc
      721 gggttgcggc ccctggccca acaggtattc catggcgagg cgaacggtag ctgtatccgt
      781 atagccgcca ccggcagcgg cgctgcatgc cggaagcgcc acggccacct tttccgccgg
      841 caaaccgggg aactcccctc ctcccgtact gaaaccctgg atcaccgctt cggtctggct
      901 cacgatgaaa tcagccgttc cctgggtata aataccccca tcgaggccga acatgctgcc
      961 gctgttgtac aactgtacct gcaggatgtc cagcgaatcg cgcagcgcat ggatcaccgg
     1021 caggtaggcg ccccagatgc ccccgaaggc actctgcccg ccctgcacgt acgcggtttc
     1081 cggtgcgatg gtcaggaaga gtttctcccc cgtcatcgtc cggaattgcg acatgatccc
     1141 gttcaacgct tcgatcaggc ggatcaccgg ctcgtcagca ggtgccgcga tggtcccgcc
     1201 cgtgatggac atggatgcgc cttccaggtc aatatcgata ccgtcgaacc cgtatgtgct
     1261 gaggatgttc atcatgctgg tgacgaactg gtcgcgctcg gcatcggaac cgaggtggac
     1321 cgtggcgttg gccccaccga tgctgatgag cacttggtgg ccgctggcct gatgcgctgc
     1381 gacctggtcg atgaaggtgg cttgcggtat tccgctgggc gtgaattgca tgttgtacgt
     1441 ggtgcccggt gccggttcgg cgaaggccac ctcgatcacg ttatagcgcg ggtcgatctg
     1501 gtccaactcc aggtacgggg ccgaggcatc gtcccagttg tgccaatagc ccaccagcgc
     1561 gggtgcgggt ccttgggccg acgcggggtc catcatggtc acccacaacg cggtgatgcc
     1621 caatagcctg tacaaggttc gcatggggcc caatgtagaa ggcgctcctt tcgcgtcgct
     1681 cgtcccgctt caattgatcg ccgcatgcct tgtagtgatc g
//
LOCUS       DAOQOC010000743         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51749_length_1719_cov_3.038462, whole genome shotgun sequence.
ACCESSION   DAOQOC010000743 DAOQOC010000000
VERSION     DAOQOC010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51749_length_1719_cov_3.038462"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PL106_11345"
     CDS             <1..260
                     /locus_tag="PL106_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNA33706.1"
                     /translation="LRMAELDAITDELARFDRLAELNVLGQCMNVIKLDHVQKRRYKE
                     RLPMIHGWIFDVRTGHLKDLGPGMEKEFMAIRKVYDLKPTV"
     gene            386..742
                     /locus_tag="PL106_11350"
     CDS             386..742
                     /locus_tag="PL106_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33707.1"
                     /translation="MSDQGPDLSQLERLCRGDRNKMAAFIDQYLSDSPALFRMLEAAC
                     DSGDARALANAAHDLRPHVHFMGAKVLLDLLVTIDRDARQDPPVVRADLVKALLPMVS
                     PLDKALRAVIHFDPGP"
     gene            820..1467
                     /locus_tag="PL106_11355"
     CDS             820..1467
                     /locus_tag="PL106_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA33708.1"
                     /translation="MIRLAIADDQPLFRRAFVLLLRDMPDVQVMFDSANGEELLTGLK
                     GNEVDIVLLDLEMPVMDGVEALRRIREEHTKVKVIVLSTHDDERSIVRMMELGANGYV
                     LKTAEPDEVETAIRSVKESGYFFSDLVSRVMLQGLVKKESVKPVFNDVDPLSERELEV
                     LRAICQEMTTTEIAGKLFLSPRTVEFHRNNILLKTGARNVAGLVVYAMTRGIYAP"
     gene            1470..>1719
                     /locus_tag="PL106_11360"
     CDS             1470..>1719
                     /locus_tag="PL106_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA33709.1"
                     /translation="MRWPQLVLFCCFTALGARAQWDTLMLPLRAITIDEGLSQGMVNT
                     ILQDRYGFMWFGTKDGLNRYDGYTFTVFRHDPNDSTSIS"
BASE COUNT          330 a          506 c          547 g          336 t
ORIGIN      
        1 gcctgcgcat ggccgaactc gacgccatca ccgatgagct tgcacgcttc gaccggctgg
       61 cggagctgaa cgtgctgggg cagtgcatga acgtgatcaa gctggaccac gtgcagaagc
      121 gccggtacaa ggagcggctg cccatgatcc acggctggat cttcgatgtg cgcaccggcc
      181 atctgaagga cctgggaccg ggcatggaga aggagttcat ggccatccgc aaggtgtacg
      241 acctgaagcc gacggtgtga tctcgctcgg atccagcgcc tccataaccg gtggcagatg
      301 atcgccgaag cgacctcgca gtcctttctt cacgttgaag catccccgtt cgcagggttc
      361 aattcggggt tggtatttta ggcgtgtgag cgaccaagga cctgaccttt cccagttgga
      421 gcggctgtgc aggggcgatc ggaacaagat ggccgccttc atcgatcaat acctgtccga
      481 cagccccgcg ctcttccgca tgcttgaagc ggcctgcgat tccggggatg cgagggcgct
      541 ggccaatgct gcgcacgacc tgcgtcccca tgtgcacttc atgggtgcca aggtgctgtt
      601 ggacctgttg gtgaccattg accgggatgc gcgacaggac ccgcccgtgg tgcgtgccga
      661 tctcgttaag gcgctgctcc ccatggtgtc cccgttggac aaggccttgc gcgctgtgat
      721 ccacttcgac ccgggcccat gaacggttcg cgcagaaccg gtgtaaatac ggggaagcga
      781 ccccggggat cgtcggaact ttcggccgtc cgaacatcca tgatccgcct cgccattgcc
      841 gacgatcagc cgctcttccg ccgtgccttc gtgctcttgc tgcgcgacat gcccgatgtg
      901 caggtgatgt tcgacagtgc caacggcgag gagctgctca ccggactgaa gggcaatgag
      961 gtggatatcg tgctactcga tctggagatg cccgtgatgg acggggttga ggcgctgcgc
     1021 cggatacgtg aagagcacac gaaggtgaag gtgatcgtgc tgagcacgca tgatgatgag
     1081 cgttcgatcg tgcgcatgat ggagctcggt gcgaacggct atgtgctgaa gaccgctgag
     1141 cctgatgagg tggagaccgc catccgttcg gtgaaggaga gcggctactt cttcagcgac
     1201 ctggtgagca gggtgatgct gcagggcctg gtgaagaagg agagcgtgaa gccggtgttc
     1261 aatgacgtgg atccgctgtc ggaacgggag ctcgaagtgc tccgcgccat ctgccaggaa
     1321 atgaccacca cggagatcgc cggcaagctc ttcctgagcc cacgtaccgt ggagttccac
     1381 cgcaacaaca tcctgctgaa gaccggcgca cgcaacgtgg ccggactcgt cgtgtatgcg
     1441 atgaccagag gcatctacgc tccctgatca tgcgttggcc tcagcttgtg ctgttctgct
     1501 gcttcaccgc cctcggtgcc cgggcgcaat gggatacgtt gatgctcccg ctccgcgcca
     1561 tcaccattga cgaaggcctt tcccaaggca tggtgaacac gatcctccag gacaggtacg
     1621 gattcatgtg gttcggcacc aaggatgggc tgaaccgtta cgacggttac acgttcaccg
     1681 tcttccgcca cgatccgaac gacagcacca gcatcagcg
//
LOCUS       DAOQOC010000744         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51753_length_1719_cov_2.932692, whole genome shotgun sequence.
ACCESSION   DAOQOC010000744 DAOQOC010000000
VERSION     DAOQOC010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51753_length_1719_cov_2.932692"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PL106_11365"
     CDS             <1..1231
                     /locus_tag="PL106_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33710.1"
                     /translation="TATDACGSSSASIDIIVGEASGSAGADMDACLGVPATLSATGGT
                     TYLWSPAASLDDPTLPTPLATPSDTTSYVVVITSGDGCETQDTLVVNVVFGPPEPTVE
                     DTAVCLGAGVQLTAYGGSSYQWAPGPGITDPTSASPMVSPTEDMYYIVFITNVCGTTD
                     DSAFVDVQQVIADAWPDTLVCPGEPVTLFASGGLAYLWAPSTALSNADSAVTVATPDT
                     PTTYTVTASNALGCEGSAMVTISHFPQPQVHASNDVRIDFGHSTVLTASGTGVLLWTP
                     GLSLSCDTCATTIASPEHTTVYTVQLTDANGCKAADQVTVFVNGNLYVPNTFTPNGDG
                     INDGFFAYATEISEFRLLVFNRWGEQIYRTNKLGPVWDGTYNGVESPIDTYVWRVDLE
                     ELSGNKRTVYGHVNLVR"
     gene            complement(1235..>1719)
                     /locus_tag="PL106_11370"
     CDS             complement(1235..>1719)
                     /locus_tag="PL106_11370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA33711.1"
                     /translation="DMLGRERMDKYWLPWLVGMPAETALAITSMIFSGLFQKLPKLRV
                     AFAHGGGAFPATVARIQHGFEMRPDLCAVDNNVGPKDHLGRFWFDSLVHDPAMLQYLV
                     DLVGADKVALGSDYPFPLGELTPGELIRGMPWDDARKEMVLSGAALQWLGMEKERFLR
                     "
BASE COUNT          325 a          593 c          511 g          290 t
ORIGIN      
        1 gaccgccacc gatgcatgcg gcagcagcag cgccagcatc gacatcatcg tgggggaggc
       61 ttccggttcc gcgggtgccg acatggacgc gtgcctgggc gtaccggcca cgctttcagc
      121 caccggtggc accacctacc tctggagccc ggcggcatcc ttggacgacc cgaccttgcc
      181 caccccgctg gccaccccct cggacaccac ctcgtacgtg gtcgtcatca cctcgggtga
      241 cggttgcgaa acacaggaca ccctggtggt caacgtcgtc ttcggaccgc ctgagcccac
      301 tgtggaggat accgccgttt gcctgggagc gggtgtgcaa ctgaccgcat acgggggctc
      361 cagctaccaa tgggcccccg ggccgggcat caccgacccc acatccgcct caccgatggt
      421 atcccccacc gaggacatgt attatatcgt gttcatcacc aatgtgtgcg gcaccactga
      481 tgacagcgcc ttcgtggacg tgcaacaggt gatcgccgac gcatggcctg atacgctcgt
      541 atgccccggc gagccggtga ccctcttcgc cagcggcgga ctcgcgtacc tctgggcccc
      601 ctccacggcg ctcagcaatg ccgattccgc ggtgaccgtg gccacgccgg acacgccgac
      661 cacgtacacg gtcacggcga gcaatgcgct gggctgcgaa ggcagcgcga tggtcacgat
      721 ctcgcatttc ccccaacccc aggtacacgc gagcaacgat gtgcgcatcg acttcggcca
      781 tagcaccgtg ctcaccgcca gcgggaccgg agtactgctg tggaccccgg ggctctccct
      841 ctcgtgcgac acctgcgcca ccaccatcgc atcaccggag cacaccaccg tgtacaccgt
      901 tcaactcacc gatgcgaacg gctgcaaggc ggcggaccag gtcacggtct tcgtgaacgg
      961 caacctgtac gtgcccaaca ccttcacgcc gaacggggac ggcatcaacg acggcttctt
     1021 cgcctacgct actgagatat cggaattccg gttgctcgtt ttcaatcgct ggggcgaaca
     1081 gatctaccgc accaacaagc tgggcccggt atgggacggc acgtacaacg gggttgaatc
     1141 acccattgat acctacgtct ggcgcgtgga cctggaggaa ttgagcggca acaagcgcac
     1201 ggtgtacggg catgtgaacc tggtgcggta gggctcaccg caagaaccgc tccttctcca
     1261 tcccgagcca ttggagcgcc gcgccgctca ggaccatctc cttgcgtgca tcgtcccacg
     1321 gcatgcccct gatgagctca cccggggtca actctcccaa ggggaaggga taatcggaac
     1381 cgagagccac cttgtcggcg cccacgaggt cgacaaggta ctggagcatg gccgggtcgt
     1441 gtaccaatga atcgaaccag aagcggccga ggtgatcctt cggcccgacg ttgttgtcca
     1501 ccgcgcagag gtcgggccgc atctcgaaac cgtgttggat gcgggccacc gtagcgggaa
     1561 aggctcctcc gccatgggcg aaggccacgc gcagcttcgg tagtttttga aacagcccgc
     1621 tgaagatcat gctggtgatg gccagtgccg tttcggccgg catgcccacc agccagggca
     1681 gccagtactt gtccatgcgc tcgcggccca acatgtccc
//
LOCUS       DAOQOC010000745         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51843_length_1717_cov_4.327316, whole genome shotgun sequence.
ACCESSION   DAOQOC010000745 DAOQOC010000000
VERSION     DAOQOC010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51843_length_1717_cov_4.327316"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PL106_11375"
     CDS             complement(<1..351)
                     /locus_tag="PL106_11375"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNA33712.1"
                     /translation="MSMFRNDKPAEAPFNTPEPVNNGKINSIMEGTSIEGEIKSDSNI
                     RIDGRVKGTITVRGRLIVGTSGVVEGDVTCQSSDIEGTVNGKITCQDLLSLKATAKLA
                     GDINTKKLAIEPGAI"
     gene            complement(526..>1717)
                     /locus_tag="PL106_11380"
     CDS             complement(526..>1717)
                     /locus_tag="PL106_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33713.1"
                     /translation="AEFKKKWGNRPNEDDWRRKDKSGSALAEEEPEEGTTEDGKDGEP
                     AWKDPETWTKDLPMDETALAASDARVCEAMYIAGTIYKEKLKDTDAAIESFEVLNGRF
                     DECRYTPESFYQLYRIYLAKEKSGSFFSLDDKGSAYYAGIIMERWPDSEFARLVSDPN
                     QLMADDQRKGREESDYAEVYKNYREGHYMPVIATCNFVIAETPNNHLLPKYHLLKALA
                     VGGMREREAFRTALLEVTSKYPGSDEAKAAQDILAQLDRSGGTSSGGAPPEVVAAPQY
                     TAGDGPHFVVVLYPNSAGPIAPQKTRISNFNQANFKSKPVSMEATVLDTTYQAIKLGP
                     FPNKADAMGYYNLFMATPDLAGLNDAGYPAFAISTANFAIFYKDKGVDAYTAFFSENY
                     LQGQ"
BASE COUNT          270 a          519 c          524 g          404 t
ORIGIN      
        1 aatggcgccc ggctcaatgg ccagtttttt cgtgttgatg tcgcccgcga gcttggccgt
       61 agccttcagg ctgagcaggt cctggcaggt gatcttgccg ttcacggtgc cttcgatgtc
      121 gctgctttga caggtgacat ccccttccac caccccgctg gtgcccacga tcagtcggcc
      181 acgcacggtg atcgtgcctt tcacgcggcc atcgatgcgg atgttgctgt cgctcttgat
      241 ctcgccctcg atggaggtgc cctccatgat gctgttgatc ttgccgttgt tcactggttc
      301 cggggtgttg aatggcgctt cggctggctt gtcgttgcgg aacatgctca tggtggcgtc
      361 aggttatcgg gagctgcaat gtcgctgcga aggtaagagc ctcagcgact tgtacatgtg
      421 cggatcatgg tcgaccaggc gtaccccgac gacagggtcc gggcactggt cgaatggact
      481 ccacaaaaag ggccgggggc gtggccggat ggcgtgcggg atcggctatt gcccctgcaa
      541 gtagttctcc gagaagaacg ccgtgtaggc gtccaccccc ttgtccttat agaagatcgc
      601 gaagttggcc gtactgatgg cgaaggccgg ataccccgca tcattcaatc cggccagatc
      661 gggcgtggcc atgaacaggt tgtagtagcc catggcatcg gccttgttgg ggaagggtcc
      721 gagtttgatc gcctggtacg tggtatccag caccgtagcc tccatgctca cgggcttgct
      781 cttgaagttg gcctggttga agttgctgat cctggtcttt tggggggcga tggggcccgc
      841 cgagttcggg tatagcacca ccacgaagtg cggcccatcg ccggcggtat actgcggggc
      901 ggccaccacc tccggcggag ccccaccgct ggaagtgccg ccggacctgt ccagttgggc
      961 aaggatatcc tgtgcggcct tggcctcgtc gctacctgga tacttggatg tcacctccag
     1021 cagggccgtt ctgaacgctt cgcgctcccg catgccacca acggccaagg ccttcagtag
     1081 atggtacttg ggaagcaggt ggttgttcgg tgtttcagcg atcacgaagt tgcaggtggc
     1141 gatcacgggc atatagtggc cctcgcggta gttcttgtat acctccgcgt aatcggattc
     1201 ttccctgccc ttgcgctgat catcggccat caactggttg gggtcgctca ccaggcgcgc
     1261 gaattcgctg tcgggccatc gttccatgat gatccccgcg tagtacgccg agcccttgtc
     1321 gtccaggctg aagaaggaac cgctcttctc ctttgccaag tagatccggt acaactggta
     1381 gaagctctcg ggcgtgtatc ggcactcgtc gaaccggcca ttgagcactt cgaaactttc
     1441 gatggcggca tcggtgtcct tgagcttctc cttgtagatg gtccccgcga tgtacatggc
     1501 ctcgcacaca cgggcatcgc tggcagccaa agccgtctcg tccatgggca ggtccttggt
     1561 ccaggtctcc ggatccttcc aggcgggctc gccatccttg ccatcctccg ttgtgccctc
     1621 ttcgggctct tcctcggcca gggccgaacc actcttgtcc ttgcggcgcc aatcgtcctc
     1681 gttgggccgg ttgccccact tcttcttgaa ctcggcc
//
LOCUS       DAOQOC010000746         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51858_length_1717_cov_3.236462, whole genome shotgun sequence.
ACCESSION   DAOQOC010000746 DAOQOC010000000
VERSION     DAOQOC010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51858_length_1717_cov_3.236462"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PL106_11385"
     CDS             <1..689
                     /locus_tag="PL106_11385"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNA33714.1"
                     /translation="ATVQITERGLDVLKQKPERIDVHFLKRFPEFMEFQSAKRNDADS
                     SEELAVPESTHTPEEVLDEAYEQIRSALGKEVLNKIQALSWMDFERLVVQLLVRMGYG
                     GSVKDAGRALAKSGDEGIDGTIKEDKLGLDVIYIQAKKWQTGNTVGRPEIQKFVGALA
                     GQGAKKGIFITTSNFSKEARDYAPRNETKIVLIDGEELTQLMIDHDLGVTPQRTYTVK
                     RIDSDFFGEE"
     gene            861..1616
                     /locus_tag="PL106_11390"
     CDS             861..1616
                     /locus_tag="PL106_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33715.1"
                     /translation="MCSRISSILLVLSLLLPSTNSAQALVLIPPTKDLFAMQVVGTGE
                     ERYINLEPDSATGNAPLDAQCAALFWHHMYLFENYANTAAYVWELVPLLPDTMLIQAR
                     IDSAFTADTAFTQLYDRVLAKEPVADLPIDSALKIAAHFFYLHSDKGKPVVHICVGIN
                     KVKMLSSEAAHPYHAAFCYQTIWGLEDHFKLLDKVKAPYSKEFKKKPPTDARILEVEQ
                     MVYTGMAQLPELRQALIDTYERTKAFLNFRLVY"
BASE COUNT          380 a          534 c          477 g          326 t
ORIGIN      
        1 gggccactgt gcagatcacc gaacgtgggc ttgatgtact gaagcagaag ccggaacgca
       61 ttgatgtcca cttcctgaag cgcttccctg agttcatgga attccagtcc gcgaaacgca
      121 atgatgccga ctcctccgaa gaactcgctg ttcccgaaag cacacacacg cccgaagagg
      181 tcttggacga ggcgtatgag cagatccgat ccgcgcttgg caaggaggtg ttgaacaaga
      241 tccaagcact ttcttggatg gactttgaac ggctcgtggt gcaattgctg gtcagaatgg
      301 gttatggtgg ttcggtgaag gatgcgggac gcgccttggc caaaagtggc gacgagggca
      361 tcgatggcac catcaaggag gataagctcg gcctcgatgt catttacatc caggccaaga
      421 aatggcagac cggtaacacc gtgggccgcc cggagatcca gaagttcgtt ggcgcattgg
      481 ctgggcaagg tgccaagaag ggcatcttca tcaccacctc caacttcagc aaggaagctc
      541 gggattacgc cccgcgcaac gagaccaaga tcgtgctgat cgatggcgag gaactcacgc
      601 aactgatgat cgaccacgac ctcggtgtca cgccgcaacg gacctacacc gtcaagcgca
      661 tcgacagcga cttcttcggg gaggagtagt gcgggctttc tgaaggctcc ttcggcgttt
      721 cagctttctc cggtcgcctc cctgcccatc ggtagccggg catgctgcac gtggccacat
      781 agccgcgcct tcagcaggct gccgctgcgc cccctcacgc ggagcacgct acattcaccc
      841 acaggtcgat cctcatccgc atgtgctcgc gcatttcatc catcctgctt gtattaagcc
      901 tgctcctgcc aagcacgaac agcgcacaag cactggtcct gatcccaccc accaaggatc
      961 tgttcgccat gcaggtcgtt ggcaccggcg aggagcgcta catcaacctg gagccggaca
     1021 gcgctacagg caacgctccc ttggatgcgc aatgcgccgc attgttctgg caccacatgt
     1081 acctgttcga gaactatgcg aacaccgctg cgtatgtctg ggagctggtg cctctactgc
     1141 cggataccat gttgatacaa gcccggatcg attccgcctt caccgccgac acggcgttca
     1201 cacagctgta cgaccgggtc ctggccaaag agccggtggc cgacctgccg atcgacagcg
     1261 ccttgaagat cgctgcgcac ttcttctacc tgcacagcga caaagggaag ccggtggtac
     1321 acatctgcgt ggggatcaac aaggtgaaga tgctttcgtc cgaagcagcg catccgtacc
     1381 acgcagcttt ctgctaccag accatctggg gcctggagga ccacttcaaa ctcttggaca
     1441 aggtgaaggc cccgtactcc aaggagttca agaaaaagcc gcccacggat gcacgcatcc
     1501 ttgaagtgga gcaaatggtt tacacgggca tggcccaact acccgagctc cggcaagcct
     1561 tgatcgacac ctacgagcgg accaaggcgt tcctgaactt ccggttggtg tactgatcgc
     1621 tactccacca ccacccgtac cacggcgcca tccgccgtgc gcaagaggta caggcccggt
     1681 tccacaccac tcaaaccgac cgcgttcgtt ccggggt
//
LOCUS       DAOQOC010000747         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51872_length_1717_cov_2.642599, whole genome shotgun sequence.
ACCESSION   DAOQOC010000747 DAOQOC010000000
VERSION     DAOQOC010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51872_length_1717_cov_2.642599"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PL106_11395"
     CDS             complement(<1..256)
                     /locus_tag="PL106_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33716.1"
                     /translation="MHTTYDLGDGTFLMAAQNNNYNREGIRLYLYHPKADSSAEILAW
                     SKPGYDSETMLPTFFTTGDRANGLVILANMGERQSWGQEVF"
     gene            complement(427..1140)
                     /locus_tag="PL106_11400"
     CDS             complement(427..1140)
                     /locus_tag="PL106_11400"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNA33717.1"
                     /translation="MRWTAAIATMALLAGCGTDRTPVIGTGDPQTRTIPVSRLSGIQV
                     SGPIDVEVTAGDTQHVEVTAQRELIDLVKLKVDNGLWRITTGRPFITDKPFTVHLTVP
                     GLNTIIVDGSGNVTSEPVFNSGKTHLEVNGSGGIAIDTLYEKLAEAWITGSGSIRLLG
                     ECDRLDARLSGSGDLMGRELCVKEADVELSGSGTMRVDVLDTLRARISGSGDLHVRGT
                     PFIAERDLTGSGTIEMISE"
     gene            complement(1144..>1717)
                     /locus_tag="PL106_11405"
     CDS             complement(1144..>1717)
                     /locus_tag="PL106_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33718.1"
                     /translation="VTSDTCTSQDMVTVSVEEGSEAGFSIRLEPNCDDIRAFLTDLST
                     GASQWLWDFGDGTTSTEQFPQHHFAYGSDIVVTLTVTDGSGCENSITQTYPATTFSEL
                     ISYEVPNIFTPNGDGKNDVFKMNTEVILGACLDMKVFNRWGQKVFESDGGNVEWGGRN
                     FAGEQCVTGTYFWTITIKDMAFSGSVYLNR"
BASE COUNT          291 a          576 c          490 g          360 t
ORIGIN      
        1 agaagacctc ctggccccaa ctctgtcgct cgcccatgtt cgccaggatc accagcccgt
       61 tggccctgtc accggtggtg aagaaggtgg ggagcatcgt ctcactgtca tagcccggct
      121 ttgaccaggc gaggatctcc gcgctgctgt ccgccttcgg atggtacagg tagagccgga
      181 taccttcccg gttgtagttg ttgttctgtg ccgccatcag gaaggtcccg tcccccaggt
      241 cgtacgtggt atgcaccagc taggtgtcgt tgcgcacgtt gtcggccagg atcggaaggt
      301 caagcactct ccaagcagtt cccggtgttc cagtatcaat ggacgagggc ggcacatccg
      361 gtgatgagcg gggagccccg ctgcaagccg ctaacagcac gacggtcgcg agggaaggca
      421 ttaccttcat tcggatatca tttcaattgt tccggatccc gtcaggtcgc gctccgcgat
      481 gaagggggtt ccccgtacgt gcaggtcgcc gctgccgctg atccgggccc gcagcgtgtc
      541 cagcacatcc acgcgcatgg tcccggagcc cgaaagttcc acatccgctt ccttgacgca
      601 cagctcccgg cccatcaggt cgccgctccc cgaaagccgg gcatcgagcc ggtcgcactc
      661 gcccagcagc ctgatggaac cgctccccgt gatccaggcc tccgccagct tctcgtacaa
      721 cgtatcgatg gcgatcccgc cactgccgtt cacttccagg tgcgttttcc cgctgttgaa
      781 cacgggctcg ctcgtcacat tcccggatcc gtccacgatg atcgtgttca gcccgggcac
      841 ggtgaggtgg accgtgaacg gcttgtcggt gatgaacggc cttccggtcg tgatccgcca
      901 cagcccgttg tccaccttca gtttcaccag gtcgatcaac tcgcgctgag ccgtcacctc
      961 aacgtgctgg gtatcgcccg ccgtgacctc cacatcgatc gggccgctca cctggatgcc
     1021 gctcaaccgg cttaccggta tcgttcgcgt ctgcggatcc cctgtgccga tcacgggtgt
     1081 tcgatccgtg ccgcaacccg ccagcaacgc catggtcgct atcgccgcgg tccaccgcat
     1141 agctcaccgg ttcaggtaca ccgaaccact gaaggccatg tccttgatcg tgatcgtcca
     1201 gaagtaggtg ccggtcacgc actgttcacc cgcgaagttg cggccgcccc attccacatt
     1261 gccgccgtcg ctctcgaaca ccttctggcc ccagcggttg aacaccttca tgtccaggca
     1321 cgcgcccagg atcacctccg tattcatctt gaacacgtcg ttcttgccat cgccgttcgg
     1381 ggtgaagatg ttcggtacct cgtagctgat cagctcgctg aaggtggtgg ccgggtaggt
     1441 ctgcgtgata ctgttctcac accccgagcc gtccgtcacg gtgagtgtca caacgatgtc
     1501 cgaaccatac gcgaagtggt gctgcgggaa ttgttccgtg ctcgtggtgc catcgccgaa
     1561 gtcccacagc cattgcgatg cgcctgtgct caggtcggtg aggaaggcgc ggatgtcatc
     1621 gcagttgggt tccaaacgaa tgctgaaccc ggcctcggaa ccctcttcca cgctcacggt
     1681 caccatgtcc tgactggtgc aggtgtcact ggtcacc
//
LOCUS       DAOQOC010000748         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51939_length_1716_cov_2.956653, whole genome shotgun sequence.
ACCESSION   DAOQOC010000748 DAOQOC010000000
VERSION     DAOQOC010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51939_length_1716_cov_2.956653"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1444
                     /locus_tag="PL106_11410"
     CDS             185..1444
                     /locus_tag="PL106_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA33719.1"
                     /translation="MRLLVTIALTLLRAKLRQSIVAAGGVTFSIAMFVALLGFMNGLN
                     ELLDGLILNRTPHVRLFNELHISARQPVERVGTQGDTVAHNFIRSLKPKDDLPRIRNA
                     EAIMDALGKDRRVLSVTPRLVAQVLFNVGTTDLTGQVFGVDVMKEVRYYMFQDYLVAG
                     DPYDIATGNNTIVLGKGLADLMAARIGDLVQVTTASGERTMLRVAGFFQSGIAEYDKV
                     QSYANIKTVQKLLRRPPSYYTDINVKLHDLNKAPALAKELATRFRVQATDIQTANAQF
                     ETGTDVRTMISYAVGIVLLIVAGFGIYNILNMIIYEKLDTIAILKATGFSGRDVRRIF
                     LLLSMIIGIVGATGGLVLGITLQHIIDNVPFNTAALPTVDTFPVDFSARYYFIAVSFA
                     LATTWVAGFFPARKAAHVDPVEIIRGK"
     gene            1441..>1716
                     /locus_tag="PL106_11415"
     CDS             1441..>1716
                     /locus_tag="PL106_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA33720.1"
                     /translation="MNTTTPVLHVEKVNRSFHDPVDLHVLKDVDLRVERGEFVSITGK
                     SGCGKSTLLYILSTMDTDYSGELYIDGEAAHQASKERLARIRNEKIGF"
BASE COUNT          337 a          572 c          519 g          288 t
ORIGIN      
        1 cgtgctgcgc accaagccgc aggccctgct ggtaccggcc tcctacatcg tcgatggcgc
       61 ttcggttctg aacggcgacg gcgaaaaaat accggtgaag ctcggtgcgc gggatatgga
      121 gcaggtggag gtgctcagcg gcatcgacgc caacacccgc atctacaaac cctgaagcgc
      181 agccatgcgc ctgctggtca ccatcgccct caccttgctg cgcgccaagc tgcgccagag
      241 catcgtggcg gccggcggcg tcaccttcag catcgccatg ttcgtggccc tgctgggctt
      301 catgaacggc ctgaacgagc tgctggacgg cctgatcctg aaccgcaccc cccacgtgcg
      361 cctcttcaac gaactgcaca tctcggcccg gcagcccgtg gaacgcgtcg ggacgcaagg
      421 cgatacggtc gcgcacaact tcatccgatc gctcaagccc aaggacgatc tgccgcgcat
      481 ccgtaacgcc gaggccatca tggatgcatt ggggaaggac cggcgggtgc tttcggtaac
      541 gccgcggctg gtggcccagg tgctgttcaa cgtgggcacc accgacctta ccggtcaggt
      601 gttcggcgtg gacgtgatga aggaggtgcg gtattatatg ttccaggact acctggtggc
      661 gggcgacccg tacgacatcg ccacgggcaa caacaccatc gtgctgggca agggcctggc
      721 cgatctgatg gcggcgcgca tcggcgacct cgtgcaagtg accaccgcca gcggcgagcg
      781 gaccatgctg cgcgtggcgg gcttcttcca gagcggcatc gccgagtacg acaaggtgca
      841 gagctacgcc aacatcaaga ccgtgcagaa actgctgcga cgcccgccca gttattatac
      901 cgacatcaac gtgaagctgc acgacctgaa caaagccccc gccctggcga aggaacttgc
      961 cacgcgcttc cgggtgcagg ccaccgacat ccagacggcc aacgcccagt tcgagaccgg
     1021 taccgacgtg cgcaccatga tcagctacgc cgtgggcatc gtactgctca tcgtggccgg
     1081 cttcggcatc tacaacatcc tcaacatgat catctacgag aagctggaca ccatcgccat
     1141 cctgaaggcc accggcttca gcgggcgcga cgtgcgtcgg atcttcctgc tgctgagcat
     1201 gatcatcggc atcgtgggcg ccaccggcgg cctggtcctg ggcatcaccc tgcagcacat
     1261 catcgataac gtacccttca acaccgccgc tttgcccacc gtggacacct tcccggtgga
     1321 cttctcggcg cgctactact tcatcgccgt cagcttcgcg ctggccacca cctgggtcgc
     1381 gggtttcttc cccgcgcgca aggccgcgca cgtggatccg gtggagatca tccgaggcaa
     1441 atgaacacga ccaccccggt gctgcacgtg gagaaggtga acagatcctt ccacgacccc
     1501 gtggacctgc acgtgctgaa ggacgtggac ctgcgcgtgg agcgcggcga gttcgtgagc
     1561 atcaccggta agagcggctg cggcaagagc accctgcttt acatcctcag taccatggac
     1621 acggactact ccggggaatt gtacatcgat ggggaggcgg cgcaccaggc cagtaaggaa
     1681 cgtctcgcgc gcatccgcaa cgagaagatc ggcttc
//
LOCUS       DAOQOC010000749         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_51953_length_1716_cov_2.534618, whole genome shotgun sequence.
ACCESSION   DAOQOC010000749 DAOQOC010000000
VERSION     DAOQOC010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51953_length_1716_cov_2.534618"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PL106_11420"
     CDS             <1..593
                     /locus_tag="PL106_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33721.1"
                     /translation="YFLLIGVLLMGLGGCKKDFHPGDTIEVVDTVSAVHARTFDTIQG
                     FGIVPIGTQSAGWDPANTLLVLPIPISLNTGDSLIATVQVLLQRNDRDPSDPDYDCER
                     DSLSVAFGTFLRATQGVAPPSALEPVQRWRPGATDGEWIKPFTLITRIRADSDMPAGA
                     VIQVFVKVWPEVGCTPFAGATCHVLSRSLALTVVHP"
     gene            590..>1716
                     /locus_tag="PL106_11425"
     CDS             590..>1716
                     /locus_tag="PL106_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33722.1"
                     /translation="MSLARFTRFPNERGLGHALLVLAGLVQGPATIRANDIEVTNVTV
                     ETIGDSVSRVEFDITWANSWRTINAPGNRDAAWIFVKYLNAVTHTWEHAYLANGGHTT
                     PFGVIDHLNGREVPGAAFDPVANRSVGVFLHRFGNGSGPFTAQGVRLVWDRGPYGIGQ
                     ADIDSVKVFAIEMVYIPQGAYYLGSGGSELGHFYTYPDESSPYQVTSEAEIPIAAVPG
                     ALYYPDTLTDPNPNYHWGDQLGPIEVGFPKGFAPFYMMKHEVAQRDFVDFLNCLTRVQ
                     QSRHTGHPNNNALLAPGVTTVNNTYVMCNSGTSLGRQKIRCDEVVDPDAPVVFYCDGD
                     GDGVGNEVDDGQWVACNYLHWRDPAAYLDWCGLRPMTELEFV"
BASE COUNT          326 a          516 c          530 g          344 t
ORIGIN      
        1 cctattttct gttgatcggt gttctgctga tgggtcttgg tggctgtaag aaggattttc
       61 atccgggaga caccatcgag gtggttgata cggtgtcggc cgtccatgcc agaacgttcg
      121 ataccatcca gggattcgga atagtgccga taggtaccca atcagcagga tgggatccag
      181 ccaataccct gctcgttctt cccatcccca tttctctcaa caccggggac agcctgatcg
      241 ccaccgtcca ggtcctgctc cagcgcaacg accgggatcc atcagaccct gattacgatt
      301 gtgaacggga ttcgctcagt gtggcattcg ggacgttcct gcgtgcgaca caaggcgtgg
      361 ctcccccatc agccctggag cccgtgcaac gctggcgtcc gggggccacc gatggtgagt
      421 ggatcaagcc cttcaccctg atcaccagga tccgtgccga cagcgacatg ccggcaggcg
      481 ccgtgataca ggtcttcgtc aaggtatggc ccgaggtggg ttgcacgccc ttcgccggag
      541 ccacctgcca tgtgctcagc cgatcccttg ctttgaccgt ggtccatcca tgagcctggc
      601 ccgattcact cgctttccca acgaacgtgg cctcggccat gcgttattgg tgctggcagg
      661 gttggttcaa ggccccgcca cgatccgggc gaacgacatc gaggtcacga atgtcaccgt
      721 ggagaccatc ggcgatagcg tcagccgggt ggagttcgac atcacttggg cgaacagctg
      781 gcgcaccatc aatgcaccag gcaaccggga tgcggcgtgg atattcgtca aatacctgaa
      841 tgcggttacg catacatggg agcatgcgta cctggccaat ggtggtcaca cgacaccctt
      901 cggtgtgatc gatcatctga acgggaggga ggtcccgggc gcggcgttcg accccgttgc
      961 caaccggagc gtcggtgtgt tcctgcaccg cttcgggaac gggtccggac ccttcacggc
     1021 ccagggggtc aggctggtct gggaccgcgg cccgtacggc atcggccagg ccgacatcga
     1081 ttccgtgaag gtcttcgcga tagaaatggt gtatataccg caaggagcgt attatctcgg
     1141 cagtgggggc tcggagctcg ggcacttcta cacctatccc gacgagtcga gcccctacca
     1201 ggtgacaagt gaagcggaga tcccgatcgc tgccgttcca ggcgccctgt actacccgga
     1261 cacgctgacg gatccgaacc ccaactacca ctggggcgat cagctcggac ccattgaggt
     1321 gggcttcccc aaagggttcg caccgttcta catgatgaag cacgaggtgg cccagcgcga
     1381 cttcgtggat tttctgaatt gcctcacgcg tgtgcagcag tcgcgccaca cggggcaccc
     1441 gaacaacaat gctttgctcg ccccgggtgt taccacggtc aacaacacct acgtgatgtg
     1501 caattccggc acatccttgg ggcggcagaa gatccgctgc gatgaggtcg tcgatccgga
     1561 tgcaccggtg gtcttctatt gcgacgggga cggtgacggg gtgggcaacg aagtggatga
     1621 cgggcagtgg gtggcgtgca actacctgca ctggcgcgat ccggccgcct atctggactg
     1681 gtgcgggttg cggcccatga ccgaactgga attcgt
//
LOCUS       DAOQOC010000750         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52000_length_1715_cov_3.309639, whole genome shotgun sequence.
ACCESSION   DAOQOC010000750 DAOQOC010000000
VERSION     DAOQOC010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52000_length_1715_cov_3.309639"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1715
                     /locus_tag="PL106_11430"
     CDS             25..>1715
                     /locus_tag="PL106_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNA33723.1"
                     /translation="MTRNEDKHPKPGAVEQAVLAAVRKSGHAGITSQQLALQLGYKEK
                     GQRYLVYDALEALQEKGQVKTGKKGRYQSTKQRDEAEGTIDIIASGAGYVRLGAEPGV
                     KAEKSTEDVFIHGSDVGMALHGDKVLIRVGGGRGNRLEGKVLKVVERRRTEFVGVIHK
                     HQGRTILVSDDQKVQRPFFIPPGDLNGATEGDKAIIGLGEWKDHRDLPRGFVKRILGR
                     AGEHNVEMHAILAEFGLPLEFPESVIQASEEIADGCTPEEIKKRRDVRSIPTITIDPD
                     DAKDLDDALSLRKLDNGNWEVGVHIADVSHYVKPGTVLDMEAAARATSVYLVDRVVPM
                     LPEKLSNNLCSLHADTDKLSFSAIFELDEKARIKNEWFGRTVMRSHHRFAYQGAQQII
                     DGKAPEDAPFRHEVLTMHKLAQVLRKDRMANGALEIGGNEVKFKLDEKDRPIEVYEKV
                     MGSANWLIEEFMLLANKRVAAWVGTLKKGGVHPFVYRIHDTPDPEKVDQLRSLAKSFG
                     YTLQATSEEDLPHAINRLLQEIKGKEEENIIKQITIRSMAKAIYSTDNIGHYGLG"
BASE COUNT          402 a          499 c          577 g          237 t
ORIGIN      
        1 ccataggcga cccgagcggc gaacgtgacc cggaacgaag acaaacatcc caagcccggc
       61 gcggtggaac aggccgtgct ggcggccgtc cgcaagagcg ggcacgcggg gatcaccagc
      121 cagcagttgg ccctgcaact gggctacaag gaaaaggggc agcggtacct cgtgtacgac
      181 gcactggagg ccctgcaaga aaaaggacaa gtgaagacag gaaagaaagg gcgctaccag
      241 agcaccaaac aacgcgatga ggccgagggc accatcgata tcatcgccag cggcgcgggc
      301 tacgtgcggc tgggagcgga acccggcgtg aaggccgaga aaagcacgga ggacgtcttc
      361 atccacggca gcgatgtggg catggcactg catggcgaca aggtgctcat ccgcgtgggc
      421 ggtgggcgcg gcaaccggct ggaaggcaag gtcctcaagg tggtcgaacg ccgacgcacc
      481 gaattcgtgg gcgtgatcca caagcaccag ggccgcacga tcctggtgag cgacgaccag
      541 aaggtgcagc ggcccttctt catcccgcca ggcgacttga acggtgcgac cgaaggcgac
      601 aaggccatca tcggccttgg ggagtggaag gaccaccgcg acctgccgcg cggtttcgtg
      661 aagcggatcc tggggcgggc cggcgagcac aacgtggaga tgcacgccat cctcgcggag
      721 ttcggtctgc cgctggaatt tcccgagagc gtcattcaag cgagcgagga gatcgccgac
      781 ggttgcacac cggaagagat caaaaagcgc cgcgatgtgc gcagcatccc caccatcacc
      841 atcgacccgg acgatgcgaa ggacctggat gacgcgctga gcctgcgcaa gctcgacaat
      901 ggcaattggg aggtgggcgt ccacatcgcc gatgtgagcc actacgtgaa acccggtacc
      961 gtactcgata tggaagccgc cgcgcgtgcg acgagcgtgt acctcgtgga ccgcgtggta
     1021 cccatgctgc cggagaagct gagcaataac ctctgctcac tccatgcgga cacggacaag
     1081 ctcagtttca gcgcgatctt cgaattggac gagaaggcgc ggatcaagaa cgagtggttc
     1141 ggccgcacgg tgatgcgctc gcaccaccgc ttcgcctatc agggcgcaca acagatcatc
     1201 gacggcaagg caccggagga tgcgccgttc cgccacgagg tgctcaccat gcacaagctg
     1261 gcgcaagtgc tccgcaagga ccgcatggcc aacggtgcgc tggagatcgg tggcaacgag
     1321 gtgaagttca aactcgacga gaaggaccgg ccgatagagg tgtacgagaa ggtgatgggc
     1381 tcggcgaact ggctcatcga agagttcatg ctgctggcga acaagcgggt ggccgcctgg
     1441 gtcggaacgt tgaagaaagg tggcgtgcat cccttcgtgt accgcatcca cgacacgccg
     1501 gatccggaga aagtggacca attgcgttcg ctggcgaaga gcttcgggta caccctgcag
     1561 gccaccagcg aggaggacct gccgcacgcg atcaaccggt tgttgcagga gatcaagggg
     1621 aaggaggagg agaacatcat caagcagatc accatccggt cgatggcgaa ggccatctac
     1681 agcacggaca acatcggcca ctacggcctg ggctt
//
LOCUS       DAOQOC010000751         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52032_length_1715_cov_2.103012, whole genome shotgun sequence.
ACCESSION   DAOQOC010000751 DAOQOC010000000
VERSION     DAOQOC010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52032_length_1715_cov_2.103012"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PL106_11435"
     CDS             complement(<1..713)
                     /locus_tag="PL106_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA33724.1"
                     /translation="MLKVMCGAACTEPPDIFGPIRERMLTCENIVKRYSTHTALDGVS
                     MDVPAGKVFGLLGPNGAGKTTLIRIITRITGPDSGRVLLDGHAMTDADVARMGYLPEE
                     RGLYKKMKVGEQALYLARLKGMDKADASKKLKDWFERWDLAGWWNKKVEELSKGMQQK
                     AQLIATLIHDPQVIIIDEPFSSLDPVNTQLVKDILKQQRDAGKTIIMCTHQMNQVEQL
                     CDKIVLVDKGEVLLYGGLQ"
     gene            complement(933..>1715)
                     /locus_tag="PL106_11440"
     CDS             complement(933..>1715)
                     /locus_tag="PL106_11440"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNA33725.1"
                     /translation="YHDYLHHAILQHSRMAKVLFVAFGLFMLAPVSIWERSHNYHHAH
                     NSKLYTSSIGSYPIVTKEKYLSSSAAERTLYMFVRHPVTIGMGYIFMFMYGMCIRSLM
                     SNKDRHWDSIVALVFHFGIGALIWWLAGFTGFFFGFLLPFVLVLALGSYLFYAQHNFP
                     GVTFTDKDGWTYADAALDSSSHMKMSPWMRWFLANIGYHHIHHLNPRIPFYRLPETYA
                     AIPELRNAKTTSLWPWDVVRCLRLKVWDPVQQRMIGRREIYA"
BASE COUNT          313 a          494 c          545 g          363 t
ORIGIN      
        1 tgctgcagac cgccgtaaag cagcacttcg cccttgtcga ccagcacgat cttatcgcag
       61 agctgctcca cctggttcat ctggtgggtg cacatgatga tcgttttgcc ggcgtcgcgc
      121 tgttgtttga ggatgtcttt gaccaattgg gtgttgaccg ggtcgagcga gctaaagggc
      181 tcatcgatga taatcacttg cgggtcgtgg atcagcgtgg caatcagttg ggctttctgc
      241 tgcatgccct tgctcaactc ttccaccttc ttgttccacc agcccgccaa gtcccagcgc
      301 tcaaaccagt ccttcagttt tttggaggca tcagccttgt ccatgccctt cagccgggcc
      361 aggtacagcg cctgttcgcc cactttcatc ttcttgtaga ggccgcgttc ctcgggcagg
      421 tagcccatgc gcgcgacatc ggcgtcggtc atcgcgtgtc catccagcag cacgcgtcct
      481 gaatccgggc cggtgatgcg ggtgatgatg cggatgagtg tggtcttgcc cgcaccgttg
      541 ggccccagca ggccgaagac cttgccagcg ggtacgtcca tgctcacgcc atccagcgcg
      601 gtatgggtgc tgtatcgttt gacgatgttc tcgcaggtga gcatgcgttc acggatcggg
      661 ccgaagatat ccggcggctc cgtgcaggct gcgccgcaca tgaccttcag caaccgttca
      721 accgggcatt gggtcgcctc tggttggtgg cctctggcta ctggcttttg gccacaggcc
      781 acgggccaca ggccacgggc tacaggccac aggctactgg ctactggctc ttcatcgcca
      841 cacggccaaa tcggcttgct gtaccacctt ggccaagcgt gatggacgtt gcatagtgct
      901 caagggccag tagccagagg cgcattgagc actcaagcgt agatctcgcg acgaccgatc
      961 atgcgctgct gcacgggatc ccacaccttc aggcgcaggc accgcaccac atcccacggc
     1021 cacagcgagg tggtcttcgc gttccgcagt tcgggaatgg ccgcataggt ctcgggcagg
     1081 cggtagaagg ggatgcgcgg attgaggtgg tggatgtggt ggtagccgat gttggccagg
     1141 aaccagcgca tccaggggct catcttcatg tggctgctgc tgtccagcgc ggcgtcggcg
     1201 taggtccatc catccttgtc ggtgaaggtg acgcccggga aattgtgttg cgcgtaaaag
     1261 agataactgc ccaaggccag caccagcacg aacggaagca ggaagccgaa gaagaagccc
     1321 gtgaacccgg cgagccacca gatgagcgca ccgatcccga agtggaagac cagcgccacg
     1381 atgctgtccc agtggcggtc cttgttgctc atgagtgagc ggatgcacat gccgtacatg
     1441 aacatgaaga tgtagcccat gccgatggtg accgggtggc gcacgaacat gtacagggtg
     1501 cgttcggcgg cgctgctgct gaggtatttt tccttggtga cgatggggta agacccgatg
     1561 ctgctggtgt acagcttgct gttgtgcgcg tggtggtagt tgtggctgcg ctcccagatg
     1621 ctcaccggtg ccagcatgaa gaggccgaag gccacgaaca gcaccttggc catgcggctg
     1681 tgctgcagga tggcgtggtg caggtaatcg tggta
//
LOCUS       DAOQOC010000752         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52229_length_1711_cov_3.629227, whole genome shotgun sequence.
ACCESSION   DAOQOC010000752 DAOQOC010000000
VERSION     DAOQOC010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52229_length_1711_cov_3.629227"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PL106_11445"
     CDS             complement(<1..1431)
                     /locus_tag="PL106_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33726.1"
                     /translation="MKNQILFRSPLLPTLAMAMAAAAHGQIAISPNAATVPNTHAMLD
                     VSDVTRGLLAPRFAWANRPVPGAPENSLLYYQTDSSTTTQEGVGYYYWDNTIPAWVHL
                     SMGPGWRIGGNAGTNPATNFIGTTNNQDMAIRTNGLERVRLRASPAGQVQVGTTAAAN
                     ERFEVNGAMRVYTANPPATASDATPVPGAIRYNSTSGAHDGYVDNPVAVGPLTQYQGW
                     YQLENAFKTRVKQKYATVPNLACRYPASTFVWNDPSALVGVVDPAGATVGTWLEFNGP
                     NPDVGTSATLETPYSTFWEDGRHQYLYRSADLEALGICPNTDVRGIAFRAAGTPAAIG
                     MRNIKVRMKNELAPAMTGVITAGLVDCANPNPYPAAPFVGVNGWNKHMFNVVNWQWNG
                     VGNNMLVEYCFDNQDWTYNSSVYFEATAYNAMFGLYCDACGHPSGAQTCYYAGPCNGA
                     NNAPSGGSQLTPGILCTGWGWNGFAGG"
     gene            complement(1445..>1711)
                     /locus_tag="PL106_11450"
     CDS             complement(1445..>1711)
                     /locus_tag="PL106_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33727.1"
                     /translation="ANWNTERGFSLGDLANQLWTTAETQALYVTELHDRLNVLEVLSN
                     DRPLDADEAAGAKGAIARMTTRTDGEKALLRHQIDQRTVHTDQR"
BASE COUNT          263 a          560 c          544 g          344 t
ORIGIN      
        1 accaccggcg aaaccgttcc agccccatcc cgtgcagaga atacccggcg tgagctggct
       61 gcccccactg ggcgcattgt tcgcgccgtt gcacggcccc gcatagtagc aggtttgcgc
      121 cccgctcggg tgtccgcacg catcgcaata caggccgaac atggcgttgt acgccgtggc
      181 ctcgaaatac accgatgagt tgtaggtcca gtcctggttg tcgaaacagt actccaccag
      241 catgttgttg cccacaccgt tccactgcca gttcaccacg ttgaacatgt gcttgttcca
      301 cccgttgaca cccacgaagg gggcggcggg gtacggattg gggttggcac aatccaccag
      361 cccggcggtg atcacccccg tcatggccgg tgccagctcg ttcttcatac gcaccttgat
      421 gttgcgcatg ccgatcgcgg ccggggtgcc agcggcgcgg aacgcgatgc cccgtacatc
      481 ggtattcggg cagataccca aggcctccag atcggcgctc ctgtacaggt actggtgccg
      541 cccatcctcc cagaacgtgc tgtacggcgt ttccagggtg gcgctcgtgc caacgtccgg
      601 gttcgggcca ttgaactcca gccatgtgcc caccgtggcg ccagccggat ccaccacgcc
      661 caccaacgca ctgggatcgt tccaaacgaa ggtgctcgcg gggtatcggc acgccaaatt
      721 gggcaccgtg gcgtacttct gcttcacccg cgtcttgaag gcattctcca attggtacca
      781 gccctggtac tgcgtgaggg gacccactgc gaccgggttg tccacatacc cgtcgtgcgc
      841 gccgctggtg ctgttgtacc ggatggcacc cggtacgggc gtggcatcgc tcgctgtggc
      901 cggcgggttc gccgtgtaaa cgcgcatggc cccgttcact tcgaaccgtt cgttggccgc
      961 ggccgtggtg cccacctgca cttgacccgc agggctcgcc cgcaggcgca cccgctccaa
     1021 cccgttggtc cggatggcca tatcctggtt gttggtggtg ccgatgaagt tggtggccgg
     1081 attggtgccc gcattgccgc cgatgcgcca gccggggccc atggacagat gcacccacgc
     1141 cggaatggtg ttgtcccagt aataataccc cacgccttcc tgggtggtgg tgctgctgtc
     1201 cgtctggtag tacaacaggc tgttctccgg ggcgcccggc accggccggt tggcccaggc
     1261 gaagcgcggg gccaatagac cgcgggtcac atcgctcacg tccaacatcg cgtgcgtgtt
     1321 gggaacggtg gccgcgttgg ggctgatcgc gatctggccg tgcgctgccg cggccatggc
     1381 catggccagt gtcgggagca gcgggctgcg gaagaggatc tggttcttca tgggatcagc
     1441 gggttcagcg ttggtccgtg tgtacggtcc gttgatcgat ctggtggcgc aacagggcct
     1501 tctccccgtc ggtgcgcgtt gtcatgcggg cgatggcgcc tttcgctccg gccgcttcat
     1561 ccgcatccag cggtcggtcg ttgctcaata cctccagcac attcaaccgg tcgtgcagtt
     1621 cggtcacgta caaggcctgc gtttcggccg tggtccacaa ctggttggcc aggtcgccca
     1681 aggagaagcc ccgttcggtg ttccaattgg c
//
LOCUS       DAOQOC010000753         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52234_length_1711_cov_3.439614, whole genome shotgun sequence.
ACCESSION   DAOQOC010000753 DAOQOC010000000
VERSION     DAOQOC010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52234_length_1711_cov_3.439614"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PL106_11455"
     CDS             <1..196
                     /locus_tag="PL106_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNA33728.1"
                     /translation="RQFTLATDVTALALTKIDGTAKGGVAIGISDQFSIPIKYLGVGE
                     GMDHLQVFDKRAFVEALFKG"
     gene            1216..>1711
                     /locus_tag="PL106_11460"
     CDS             1216..>1711
                     /locus_tag="PL106_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNA33729.1"
                     /translation="MANTVLHKAATRGGADHGWLKAKHTFSFANYYDPQRMHFGVLRV
                     LNDDRIAAGMGFGTHPHDNMEIITIPLSGTVAHKDSMGSSGTISPGEVQVMSAGTGVT
                     HSEFNHLQDEELRLLQIWLFPNKRGVTPRYDQMSLDVKDRRNSLQQILSPRADDAGVW
                     IHQNA"
BASE COUNT          364 a          518 c          509 g          320 t
ORIGIN      
        1 ccgacagttc accttagcta ccgacgtcac cgcattggcg ctcaccaaga tcgatgggac
       61 cgccaagggc ggcgtggcca tcggcatcag cgaccagttc agcatcccca tcaagtacct
      121 cggggtgggt gagggtatgg accatctgca ggtgttcgac aagcgcgcgt tcgtggaagc
      181 gctcttcaag ggctgatcgg ccccgcgcct ttccgtgccc tgacaggtgt gcgggccgag
      241 caggaaacat cgtgcagggc atgaaggata atgtccggga gggtggtcgc tggagtgtgg
      301 cggtcgaacg tatgtatggc catacataca tcaggctatg atagcacttg gcgaagggcg
      361 gatccgtacg gatcaggctc gggggaccgg ctttccgcaa gtcggtagca gaacgtatgt
      421 atggccatgc atacgtcggg gtataagagc acctgggtgc actgggactc gcacggctca
      481 tgctcgagag cccggagcac agtagacatg tgcagaacat atgtatggcc atacatacgt
      541 caagctgtgg tagtacctga caaaggatgg ccccgtacgg atcgttctcg agaacccagt
      601 tatctcctgg ccctagagaa cgtatgtatg gccatacata cgtcaaggac aagttgaaat
      661 gatgcgttgt tcagcgacgc gtggatgaca caccggatcc tttccatgac cctccccatc
      721 aggatctccg aacatgctcc gtaaagagcc aagtaccgcg ctcgcgcgtc tgatccctgg
      781 tggcatcggc catatattca gctcagatcc acggtgagag ccgggatccg cgaccggata
      841 cccgaccatg tggggggatg ggagatcccg agtgcgaata gggcggatga ccggaaggct
      901 ccgggtggtc cacaacccac aggacctggg tgtcgtcgct tctcgatggc ttccctgaaa
      961 accggtatgg ccatccatcg gccgcctggt tttcttgtcc tggttcaatg cttggccccc
     1021 tgcggtcgcg gacctttgtg ccatcaacgt caacgcacca tggatcgcaa agacttcctc
     1081 cggaaaggcc tgctgggcac gggtatgctg gctgccagcc ccttgctcgc cgacctggtg
     1141 gagaacggga tcgatgaact gcgcccgctc gacgttgtcg gcttcaacca cttgccctcc
     1201 ccaacacccc gcatcatggc aaacaccgtc ttgcacaagg ccgccaccag gggcggtgcc
     1261 gatcacggct ggctcaaggc caagcacacc ttcagcttcg ccaattacta cgacccccaa
     1321 cgcatgcact tcggtgtact gcgcgtgctc aacgacgacc gcatcgccgc cggcatgggc
     1381 ttcggtacgc acccgcacga caacatggag atcatcacca tcccgctcag cggtaccgtg
     1441 gcccacaagg acagcatggg cagcagtggt accatcagtc ccggcgaggt gcaggtgatg
     1501 agcgccggca cgggcgttac gcacagcgag ttcaatcacc tgcaggatga ggaactgcgg
     1561 ctgctgcaga tctggctgtt ccccaacaag cgtggagtga cgccccgcta cgatcagatg
     1621 agcttagatg taaaggaccg ccgcaacagc ctccaacaga tcctctcacc cagggccgac
     1681 gacgcgggtg tgtggatcca ccagaatgct t
//
LOCUS       DAOQOC010000754         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52309_length_1710_cov_3.157100, whole genome shotgun sequence.
ACCESSION   DAOQOC010000754 DAOQOC010000000
VERSION     DAOQOC010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52309_length_1710_cov_3.157100"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..385
                     /locus_tag="PL106_11465"
     CDS             38..385
                     /locus_tag="PL106_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HNA33730.1"
                     /translation="MPPTAYHVYVIELSRRVFTEDRKFREANPQFNGVLECLYVGMTS
                     RTPEERFQQHRTGHRNAKGHKLSSNIVQKYGRYLRPSLYRDIGPLSRAEALEVEKGLT
                     LELRRKGYAVWSN"
     gene            complement(685..>1710)
                     /locus_tag="PL106_11470"
     CDS             complement(685..>1710)
                     /locus_tag="PL106_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33731.1"
                     /translation="GAFFSGKLWIESATATVRSMELVCDRCTRHPFLPMGPGDELRDL
                     SIHYHQTFSTWESRTVVNTVALEYAYTYHTGTASARLAERDPAFRNDWKFRTKSILHL
                     YAPGEKFILPLFRYDAGQMDYRKVLSMPYDSVFWTNAPSLVRTDRQQRDETLFAQEGL
                     LLGTREMDPMDTGRSGFFESNYAFWSADKRIRLKNMLDSAAAAPRPATAESATATADQ
                     LNLVVQLYLNVDRTPEGYRSFSATVFDGFNSWCHLPDKQRSDVLLNIYFDLCEVERRR
                     MQVALEAPGLSLDRIRAIHAEAETAMERNTSAFLKDTHYGADQQAMKHWNDLVRRELG
                     VDNFKIF"
BASE COUNT          334 a          519 c          519 g          338 t
ORIGIN      
        1 tgttcctttc gaccgcgaaa ccctcttttg cggtgcgatg ccgccgaccg cctaccacgt
       61 atacgtgatc gaactctcca ggcgtgtctt caccgaggac cgcaagttcc gcgaggcgaa
      121 cccgcagttc aacggggtgc tcgaatgcct gtatgtcggt atgacgagca ggacacccga
      181 ggaacgtttc cagcaacaca ggaccgggca tcgcaacgcg aaggggcaca agctctcctc
      241 caacatcgtg cagaaatacg gtcgctacct gcgtccgagc ctgtaccggg acatcggacc
      301 gttgagtcgc gcggaagcgc tcgaggtgga gaaaggactc acgcttgaac ttcggcggaa
      361 gggctacgcc gtctggtcca actgaggtgc gcccggccgc agcgcgaggc tgcacacatg
      421 tgaccggcgc tcaggtgttt cccgagtgcc atggctcctc tccgcctccg gcgatacctg
      481 gaaattatcg tgaaaggatc atgcacaagt ctcggcggaa gcctgacggc cggaggccgg
      541 actgcgcgcg gcactcggga aacacccgag cgcgggaact gtgcataccc gagcgcgggt
      601 gggtgtcacc ctgagcttcc tcgctgcttc agggtcccgg atccacttga acgcacagcg
      661 cggttgcgga gagcctgcgg gagatcaaaa gatcttgaag ttgtccacac cgagttccct
      721 gcgcacaagg tcgttccagt gcttcatggc ctgctggtcc gccccgtagt gcgtgtcctt
      781 cagaaaggcg ctcgtattgc gttccatcgc tgtttcggcc tcggcatgga tggcgcggat
      841 ccgatccaac gaaaggccgg gtgcttccag tgcgacctgc atgcgccttc gctccacctc
      901 gcagaggtcg aagtagatgt tcaggagcac gtccgaccgt tgcttgtccg gcaggtggca
      961 ccaggagttg aagccatcga agactgtggc gctgaagctg cgataccctt ccggggtgcg
     1021 gtccacattg agataaagct gcaccaccaa attcagttgg tcggccgtgg ccgtggcact
     1081 ttccgcagtt gcggggcgtg gagcggctgc ggcactgtcc aacatgttct tcaggcgaat
     1141 gcgtttgtca gctgaccaga acgcgtaatt gctctcgaag aagccgctgc ggcccgtatc
     1201 catgggatcc atttcgcgtg tgcccaacag caggccctcc tgcgcgaaca acgtttcatc
     1261 ccgttgctgc cgatcggttc gcaccaggct tggggcattg gtccagaaca cgctgtcgta
     1321 cggcatggaa agcactttgc ggtagtccat ctgcccggca tcgtagcgga acaagggcag
     1381 gatgaacttt tccccggggg catagagatg caagatgctc ttggtccgga atttccagtc
     1441 gttccggaaa gccggatccc gctctgcaag ccgcgctgat gccgtcccgg tatgataggt
     1501 gtatgcatac tccagtgcca ccgtgttcac caccgtacga ctttcccatg tggaaaaggt
     1561 ctggtgatag tgaatggaga gatcgcgcag ctcgtccccg ggccccatgg gcagaaacgg
     1621 atggcgcgtg catcggtcac aaaccaactc catgcttcgc accgtggccg tggccgattc
     1681 gatccagagt ttcccgctga aaaaagcccc
//
LOCUS       DAOQOC010000755         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52319_length_1710_cov_2.735952, whole genome shotgun sequence.
ACCESSION   DAOQOC010000755 DAOQOC010000000
VERSION     DAOQOC010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52319_length_1710_cov_2.735952"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="PL106_11475"
     CDS             complement(<1..1498)
                     /locus_tag="PL106_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNA33732.1"
                     /translation="MNSRINEALVLGLTLLSVYHAKGQIVPPGHGPGWALAFGGVNQH
                     ANTLANPITIGPAITIEAWINAGAFNYGEIYALCDDPNSACNGMDYKFLDVRVNPSGQ
                     IEIGLSPTAWSSRRIYLISSVAIPPNTWTHVAGTMDLGTNSGAIYINGVSVPLTSNNI
                     GSGTGSFTGSPAIGALQVSGATTGVQYPFTGKIDELRIWNTIIGQTSIRDYMCRNNLT
                     GHPDLANLQALYHVEESGPNTATVWDHIGNHHATLTNVSVNDAYGLSEAALGDTSSHT
                     YSGPNLGLGTPTESFYISSNSNAGKHIYHVDPPGANVDGTVPSGLDAAGGGCFGVFLA
                     EAPSAFYLVNYSYTGNPELDGTAAEPGSVLLRRDGPLGTPWTQQAPQWLNQTVNVIDL
                     QGTWQRHMQWAGAYTPVSCDNWTLTIDTDDNGNETTWQIADATTLSVIASGGPYPDNS
                     SNTETICVPPGGCYRLTVNDSGNDGITDGGWKLLDNNGRRILDNVDNGG"
BASE COUNT          297 a          483 c          534 g          396 t
ORIGIN      
        1 agccaccgtt gtccacattg tccaggatgc ggcggccgtt gttgtccagg agtttccagc
       61 caccatcggt gatcccgtcg ttcccactgt cgttcacggt aagccggtaa cagcctcccg
      121 gtggcacgca gatggtctcc gtgttcgagc tgttgtccgg gtagggccca ccgctggcga
      181 tcacactgag tgtggtggcg tcggcgatct gccaggtggt ttcgttgccg ttgtcgtcgg
      241 tgtcgatcgt caaggtccaa ttgtcgcagg acacgggtgt gtacgctcct gcccattgca
      301 tgtggcgttg ccaggtgcct tgcaggtcga tcacattcac cgtttggttc agccattgag
      361 gagcctgctg cgtccacggc gttcccagtg gtccatcgcg tcgcagcagt accgaaccgg
      421 gttccgcggc ggttccgtcc aactccgggt tgccggtgta gctgtagttc acaagataga
      481 atgccgatgg tgcttcagcg aggaagacac cgaagcaccc gccacccgcc gcatccaaac
      541 cgctgggcac agtgccatcc acgttggcgc cggggggatc gacatgatag atgtgtttgc
      601 ccgcattgga gttggagctg atatagaagc tttccgtggg tgtaccgagc ccgaggttcg
      661 gcccgctgta cgtgtgggaa ctggtgtcgc ccaaggccgc ctcgctcaga ccgtacgcat
      721 cattcacgct cacgttggtg agtgtcgcgt ggtggttgcc gatatggtcc cataccgttg
      781 cggtgttcgg acccgattcc tccacatggt acagggcctg caggttggcg aggtccgggt
      841 ggcccgtgag gttgttgcgg cacatatagt cgcggatgct cgtttgaccg attatggtat
      901 tccagatgcg tagctcatcg atcttgcctg tgaagggata ctgcacgccc gtcgtagcac
      961 cgctcacctg caaggccccg atcgccgggc ttccggtgaa actgcccgtg cccgaaccta
     1021 tgttgttcga ggtcagcggg acggatacac cattgatgta gatggcaccg gaattagtac
     1081 ccagatccat ggttcccgca acgtgcgtcc aggtgttggg ggggatggcc acggaactga
     1141 tgaggtagat gcgcctgctg gaccacgcag tgggcgataa ccctatctcg atctgtccgg
     1201 acgggttcac acgcacgtca agaaatttgt aatccatacc gttgcatgcc gagttgggat
     1261 cgtcgcacag ggcatatatc tccccatagt tgaacgctcc cgcgttgatc catgcttcga
     1321 tggtgatcgc cggccctatc gtgatcgggt tggccagtgt gttcgcatgc tggttcacac
     1381 caccgaacgc cagggcccag cccgggccat gaccaggtgg cacgatctga cctttcgcgt
     1441 ggtatactga cagtaaggtc agaccgagca cgagcgcttc attgatcctt gaattcatgg
     1501 tccaggggtt ctttgtgttc gtttcgttcg gtaccgggtc gcctcctcat ggttggatcg
     1561 cacgaagcga tcgcctgtac cagcggaaag cgggtagggg cagccctcgg gtcggcccat
     1621 gatggtgcgt tggcgtggtc cggttaaaag tgctgcatca accccttttg ctgacccggt
     1681 tgaaatacaa gtgggttgat ggcaatgcgg
//
LOCUS       DAOQOC010000756         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52384_length_1709_cov_3.226119, whole genome shotgun sequence.
ACCESSION   DAOQOC010000756 DAOQOC010000000
VERSION     DAOQOC010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52384_length_1709_cov_3.226119"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1369
                     /gene="rfbB"
                     /locus_tag="PL106_11480"
     CDS             278..1369
                     /gene="rfbB"
                     /locus_tag="PL106_11480"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNA33733.1"
                     /translation="MQRNILITGGAGFIGSHVVRRFITNYPQYRIINLDALTYAGNLE
                     NLRDIENAPNYSFVKCDIRDAEGLKKVFADHRPDGVIHLAAESHVDRSITDPMAFVTT
                     NVIGTVTLLNAAREAWSHAAKTGSGNGAKGAFEGKRFYHVSTDEVYGSLHDDSLFLET
                     TPYDPQSPYSASKAASDHFVRAYGNTYKLPFVVSNCSNNYGSHHFPEKLIPLMINNIR
                     NRKPLPVYGKGENVRDWLWVEDHASAIDIIFHKGKNCETYNIGGHNEWKNIDLVHLLC
                     SIMDRKLGRPEGESAKLITYVTDRAGHDLRYAIDASKIERDLGWKPSITFEVGLERTV
                     DWYLANSEWLDHVTSGAYQLYYAAHYTGR"
     gene            1428..>1709
                     /locus_tag="PL106_11485"
     CDS             1428..>1709
                     /locus_tag="PL106_11485"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33734.1"
                     /translation="MGFLGKFFGSRQPDLGPADLSVLRCDVHSHFIPGIDDGAQTLEQ
                     SVELISAMHDLGYTKVITTPHVMADGYRNTPEIILEGLEEQARQVVHSGQ"
BASE COUNT          391 a          541 c          480 g          297 t
ORIGIN      
        1 accaaggagg tgaaaggacc gtacgatgcg atcatcgtgg ccgtgaacca cgccgaatac
       61 gccgccaaga acgaagcctg gttcaggagc atgctcacac cgaagggcgt gctggtggac
      121 ctcaagggca tcttccgcaa gcaggtgaca gcgttgaact actggagttt gtaatgaagt
      181 tcaccacaga ggcacagagg acacagagct acattatgga gcgcatcctg gccttactcc
      241 gtgccctctg tgcctctgtg gtgaagatga acagagcatg cagcgcaata tcttgatcac
      301 cggcggcgcc ggcttcatcg gcagccacgt ggtgcgccgc ttcatcacga attatccgca
      361 gtaccgcatc atcaacttgg atgccctcac ctacgcgggc aacctggaga atctgcggga
      421 catcgagaac gcacccaact attccttcgt caagtgcgac atccgcgatg cggaaggatt
      481 gaagaaggtc ttcgccgatc accgccccga tggtgtgatc cacctcgccg ccgaaagcca
      541 cgtggaccgc agcatcaccg atcccatggc cttcgtcacc acgaacgtga tcggaacagt
      601 gacgctgttg aacgctgcgc gcgaagcttg gtcgcacgcc gccaaaaccg gctccggcaa
      661 cggtgcgaaa ggcgccttcg aaggcaagcg cttctatcat gtgagcacgg acgaggtgta
      721 cggctcattg cacgacgaca gcctcttcct ggagaccaca ccctacgacc cgcaaagccc
      781 gtacagcgcc agcaaagccg ccagcgacca cttcgttcgc gcctacggca acacgtacaa
      841 gctgccgttc gtggtgagca actgcagcaa caactacggc agtcatcact tccccgagaa
      901 gttgatcccg ctcatgatca acaacatccg caaccgcaag ccattgcctg tctacggcaa
      961 gggcgagaac gtgcgcgact ggttgtgggt ggaggaccac gcatccgcca tcgacattat
     1021 ctttcataag ggaaagaact gcgagaccta taacatcggc ggccacaacg agtggaagaa
     1081 catcgatctg gtgcatctgc tctgttccat catggaccgc aagctgggcc ggccggaggg
     1141 cgaaagcgcc aagctcatca cctacgttac ggaccgcgcc gggcacgacc tgcggtacgc
     1201 gatcgacgca tccaagatcg aacgcgacct gggctggaag ccgagcatca ccttcgaggt
     1261 gggcctggag cgcacggtgg attggtatct cgccaactcc gagtggctgg accacgtgac
     1321 ttccggcgcc taccagctgt actacgccgc gcattacacc gggcgctgaa aaatcctgaa
     1381 cacgggcgca tcgctcagcg ttcttcgcgt cctttgcggt accaaccatg ggcttcctcg
     1441 gcaaattctt cggttcacgc cagcccgacc tcggcccggc ggacctgagc gtattgcgct
     1501 gcgacgtcca cagccacttc attcccggca ttgatgatgg cgcgcagacc ctggagcaga
     1561 gcgtcgaact catcagcgcg atgcatgacc tgggttacac caaggtgatc accacgcccc
     1621 acgtgatggc cgacgggtat cgcaataccc cggagatcat cctcgaaggg ctggaagaac
     1681 aggcccggca ggtcgtgcat tccggccaa
//
LOCUS       DAOQOC010000757         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52578_length_1706_cov_2.729255, whole genome shotgun sequence.
ACCESSION   DAOQOC010000757 DAOQOC010000000
VERSION     DAOQOC010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52578_length_1706_cov_2.729255"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1706
                     /locus_tag="PL106_11490"
     CDS             15..>1706
                     /locus_tag="PL106_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33735.1"
                     /translation="MRIVVGGFAPRTLSLEEARIMGVVFLDQPTGELNEVVVEPWPRE
                     RDRHALASTAVLDSTLTADFERSSLRGSAQWAPGVQWDERGHGGSPRLSIRGSMARSV
                     YGTRGVKAYWGPFPITLADGSTPIELLDPLLVGSLDIGRSVGNPNYGSAPSGLVLAGG
                     PFREGPGIDAMVEATGGSFGYYRLGALGRTNKNGTSLTVGVVHQRNTGYRQQESSARD
                     QVFLTAAYRYKRTSTRVFATWQNAGWDLPGSVDEQTMMSAPRTANPYSILVDAHIEKQ
                     QLMTGVSNELRLGGITVRTGVHGQMIDKTNPYGTSPLNCGYKEETARALGARLTIGAD
                     RLFTLPLAWNIGLEALSERDHLRELYYTNGIKGDIKLNGDTRISNLNAFATTVARLGR
                     TTTLHAGVGLERTGYDHTDEMRRTRAERETSPVVLPYSGVEQDIGQGYRLRLRYAESI
                     SRATVWELLGADGTLSDQLRGERVREWELGADNAGSGGPVRADVSLYHRLVKDMITQR
                     PGYNDGPAIYDNADQASIMGMELLVNGPVITRERARMEVLGTCTVSASDLRNNGQS"
BASE COUNT          330 a          525 c          587 g          264 t
ORIGIN      
        1 ggccttgcga ctccatgcgg atcgttgtgg gcgggttcgc cccacgaacg ctcagcctcg
       61 aagaggcccg gatcatgggc gtggtgttcc tggaccagcc gacaggcgaa ttgaatgagg
      121 tggtggtgga gccgtggcca cgcgagcgcg accggcatgc cctggcctcc accgccgttc
      181 tggacagcac gcttaccgcg gactttgagc gctcctcact tcgtgggtcg gcccaatggg
      241 caccgggagt gcaatgggac gagcgtgggc atggcggcag cccccgcctg agcatccggg
      301 gcagcatggc ccgttcggtg tacggcaccc gcggagtgaa ggcgtattgg ggtccgttcc
      361 ccatcaccct ggcggacggc agcacgccca tcgaactgtt ggatcccctg cttgtgggat
      421 ccctggacat cgggcgcagc gtagggaacc cgaattatgg aagtgccccc agtggcctgg
      481 tgctggctgg cggaccgttc cgcgagggcc cgggcatcga tgccatggtg gaggcgacgg
      541 ggggctcgtt cgggtattat cgactggggg ccttgggtcg caccaacaag aacggcacaa
      601 gtctcacggt cggcgtggta caccagcgca acaccggata ccgccagcag gaatcctccg
      661 cacgcgacca ggtcttcctg accgcggcat acaggtacaa gcgcacctcc acacgggtat
      721 tcgccacctg gcagaacgcg ggctgggacc tgccgggcag cgtggacgag cagaccatga
      781 tgagcgcacc tcgcaccgcg aacccgtatt cgatcctggt ggatgcgcac atcgaaaagc
      841 aacagctcat gaccggtgtg tccaacgagt tgaggctggg cggcatcacc gtacgcacgg
      901 gcgtgcatgg ccagatgatc gacaagacga acccgtacgg caccagcccc ctgaactgcg
      961 ggtacaaaga ggagaccgcc cgtgcgctgg gggcccggct caccatcggc gccgaccggc
     1021 tcttcacgct tcccctggcc tggaacatcg gcctggaagc gttgagcgag cgtgaccact
     1081 tgcgcgagtt atactatacg aacgggatca agggagacat caagctgaac ggcgacacgc
     1141 ggatctccaa cctgaacgcc ttcgccacca cggtcgcccg gctggggcgg accaccacct
     1201 tgcatgcagg cgtgggtctg gagcgcaccg gctacgacca tacggatgag atgcggcgta
     1261 cacgcgccga acgggagaca tctccggtcg tgctgcccta cagcggggtg gaacaggaca
     1321 tcggtcaggg ttatcggctg cggctgcgct acgccgaaag catcagccgg gccaccgtgt
     1381 gggaactgct gggggccgat ggaacgctgt cggaccagct gcgtggtgag cgggtacgcg
     1441 aatgggaact cggtgcggac aatgcgggga gcggcggtcc ggtgcgtgcg gatgtgagcc
     1501 tttaccaccg cctggtgaag gacatgatca cccagcgacc cgggtacaac gatggtcccg
     1561 ccatctacga caacgcggac caggcgagca tcatgggcat ggaacttctg gtgaacggcc
     1621 cggtgatcac gcgcgaacgg gcacggatgg aagtgttggg gacatgcacg gtcagcgcaa
     1681 gcgatctgcg gaacaacggc caaagt
//
LOCUS       DAOQOC010000758         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_52882_length_1701_cov_3.755164, whole genome shotgun sequence.
ACCESSION   DAOQOC010000758 DAOQOC010000000
VERSION     DAOQOC010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52882_length_1701_cov_3.755164"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PL106_11495"
     CDS             complement(<1..1406)
                     /locus_tag="PL106_11495"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA33736.1"
                     /translation="MIFLCRSGVEGQDPRFQHLTTEDGLSDNAVTCVYEDRGGSIWVG
                     TENGLNRFDGSSVQIIPGTAMHITAVTGDGDGTIWAATKDKGLLRVDPDLGSIDRFHQ
                     GDTTGTSLCCDLLTAIHDLNDTTLLLGAREHALLFFDKRTFTCTYWQDSLDLSPHRAS
                     PDPVHDQGWCHALLPLNDTLLWIGFLHNGQSLLVDRRSLRTSERLSIRRAGSESQTSA
                     ALFGDTLFSGGWQNDIDAVRSRSGFNRSIHAPGSFMRLPVADEVNVLLRWTDHELLAG
                     TRGNGLFLIDIRSGGMRRLLHMRGDANSLADDRIRALYRDRQGTLWVGTANGLDRFVP
                     RIWSMKRTDLFNDPRLDHPDLFFHRAEPLGEHGARIFSSGGFFLVEDEAQVRHVPVEY
                     RGRELQPTFLGPLAGTRRLLGTEYGIVEWDEALGHISGVRAPRVEKGTTYPVGGMYQV
                     HGLSFVDIHGHRVLLAAA"
BASE COUNT          360 a          533 c          505 g          303 t
ORIGIN      
        1 aaggcggcgg ccaaaagcac cctgtgcccg tgtatgtcca cgaaggaaag accatgcacc
       61 tgatacatgc ctccgacggg ataggtggtt cccttctcca ccctcggggc acggactccg
      121 gagatgtggc cgagcgcctc atcccactcc acgataccat actcggtgcc gaggagtcgc
      181 cgcgttccgg ccagaggacc caggaaggtc ggctgcagtt cgcggcccct atattcgacc
      241 gggacgtggc gcacctgcgc ctcatcctcc acgaggaaga atcctccgga cgagaagatc
      301 ctggcaccgt gctcgccgag aggttcggcc cggtggaaga agaggtcggg atgatccagt
      361 cgtgggtcgt tgaagaggtc ggtccgcttc atggaccaaa tgcgggggac gaaccggtca
      421 aggccattgg cggtgcccac ccagagcgtg ccctgccggt cacggtacaa ggccctgata
      481 cggtcatcgg ccaacgagtt cgcgtccccg cgcatgtgca agagccggcg cattccaccc
      541 gaacgtatgt cgatgaggaa cagcccattc ccacgtgtac cggccaggag ttcatggtcg
      601 gtccaccgca agagcacatt gacctcgtcc gccacgggca gtcgcatgaa ggacccagga
      661 gcatggatcg acctgttgaa gcctgagcgt gaccgcaccg catcgatgtc gttctgccaa
      721 ccaccgctga agagcgtatc gccgaaaagc gccgcggagg tctgggattc cgaacccgcc
      781 cttcggatac tcaaccgctc gctggtacgc aaggaacgcc ggtccaccag gagtgactgt
      841 ccgttgtgca gaaagccgat ccaaagcagg gtatcattca acggcaaaag cgcatggcac
      901 cagccttgat catggaccgg atccgggctt gcccggtgcg gtgacaggtc gagactgtcc
      961 tgccagtacg tgcacgtgaa tgtccgcttg tcgaagaaca ggagcgcatg ctcccgcgcg
     1021 ccaagaagaa gggtggtgtc gttgaggtcg tggatggctg tgagaagatc gcagcagagc
     1081 gacgtcccgg tggtgtctcc ctggtggaac ctgtcgatcg atccgaggtc gggatccacg
     1141 cgcaacaacc ccttatcctt ggtggccgcc cagatcgtgc catccccatc gcctgtaacg
     1201 gcagtgatgt gcatcgcggt accgggtatg atctgaacgc tgcttccatc gaatcggttc
     1261 agcccgttct ctgtaccgac ccagatggat cctccgcggt cctcatacac acaggtgacg
     1321 gcattgtcac tgaggccatc ctccgtggtg aggtgttgaa agcggggatc ctggccctcc
     1381 acgcccgaac ggcacaggaa gatcattacc aaaacacata actccctcct gcgcatgacc
     1441 gcaaaatacc gcgatggaac gatccgcagc gatcccgatc gtacgacatg tccgtttgca
     1501 acggaaccaa ccatgcgaac ggatcgtccg gaatgtcatc accgtcagaa aaacactcgc
     1561 cccccacctt gcggcagggg gcgagcgtct tgtatcctat cagcttactg gatcaccagg
     1621 cgctgtgtgc gaagtgtttc gccagtgcgc acattgacga ggtacagccc aggggccaga
     1681 tcccgctcga agctcatgct c
//
LOCUS       DAOQOC010000759         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53013_length_1699_cov_4.125912, whole genome shotgun sequence.
ACCESSION   DAOQOC010000759 DAOQOC010000000
VERSION     DAOQOC010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53013_length_1699_cov_4.125912"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PL106_11500"
     CDS             complement(<1..857)
                     /locus_tag="PL106_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33737.1"
                     /translation="MSSLPASPPLRQHYNAARLRTHTWDQLKLAAMDLDEGRGEHGPV
                     AAILHDLFAIELYWAWPGRETVRHMQDMLRAREYHGLSLAVNHIVRLLSSGAFRNDVP
                     SMLTPQPVQHDMLIEEEEKTRPNYFEVLFVDEFDEVEEAALKQKLLKCRDKNDPFVYD
                     VVVVRTVQDALIALLFNHNIQAMVTRFGVPYPGDNHKGVLREFTRAIDLLQPDGISRA
                     STGPWLGRICRWFRPEVDRYCITDTPNDGLDDDILTTFKRIFYRTEDLTELHLTLMRG
                     TQDKYDTPF"
     gene            972..>1699
                     /locus_tag="PL106_11505"
     CDS             972..>1699
                     /locus_tag="PL106_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33738.1"
                     /translation="MRAAPPTAAGDAADADWHRSFPWAALSLVLGLLYRGLALGQCAN
                     DNTLYTTVTPPCPGTGTVVCMYGGEYALVNVVMGNTYTFSTCDFASFDTEITLYNNSG
                     GAALGYNDDGCAPSSIVSWTATFTGQLRVLVDAWPCGSMASCADLTITCAAAGGCPAG
                     EVADCNGNCVPTSWLGDGLCDDGVFTYNGVPIYLNCASLNFDNGDCGGGGCAAGEIPD
                     CNGNCAPASWVGDGTCDNGARSYNG"
BASE COUNT          303 a          479 c          569 g          348 t
ORIGIN      
        1 aagaaggggg tgtcgtattt gtcctgggtg ccgcgcatca gcgtgaggtg cagctcggta
       61 aggtcctcgg tacggtagaa aatgcgtttg aatgtggtca gtatgtcatc atccagccca
      121 tcgttgggcg tatccgtgat gcagtagcgg tccacctcgg gccggaacca acggcagatg
      181 cgcccgagcc acgggccggt gctcgccctg ctgatcccat cgggttggag gaggtcgatg
      241 gcgcgggtga attcgcgcaa cacgcccttg tggttatcgc ccggataggg tacgccgaag
      301 cgcgtgacca tggcctgtat gttgtggttg aacaacaagg cgatcaatgc gtcctggacg
      361 gtgcgcacca cgaccacgtc gtacacgaac gggtcatttt tatctcggca tttcagtaat
      421 ttctgtttga gggcggcctc ttccacctcg tcgaattcat ccacgaagag cacctcgaaa
      481 taattgggcc gcgtcttctc ctcctcctcg atgagcatgt catgttgcac gggctgcgga
      541 gtgagcatgg agggcacgtc gttccggaac gcaccgctgc tgagcagacg cacgatatgg
      601 ttcaccgcga ggctcaggcc gtggtactcc ctggcacgca acatgtcctg catgtgcctc
      661 accgtctcgc gaccgggcca ggcccagtac aattcaatgg cgaacaggtc atggaggatg
      721 gccgccaccg gaccatgttc accgcggcct tcgtcgaggt ccatcgcggc cagcttgagc
      781 tggtcccatg tatgggtgcg caggcgggcg gcgttgtagt gctggcgcaa cgggggggag
      841 gcgggcaggg aggacatgca ggatgatcgg tatgcgggaa agttaggaga gcttgggctc
      901 atcgtgtggg gccgaccggg gtttgatggc cacttgcacg aatggtgggt tttcctattc
      961 ttgtggatac catgcgagcg gctcctccaa cagcggcagg cgatgcagcg gatgctgact
     1021 ggcatcgatc gttcccatgg gcagccctgt ccctggtcct cgggctgctg taccgggggc
     1081 tggcccttgg ccaatgtgcg aacgacaata cgctgtacac cacggtgaca ccaccttgcc
     1141 cgggtacggg tacggtggtc tgcatgtacg gcggcgagta cgcgctcgtg aatgtggtga
     1201 tgggcaacac gtacacgttc agcacgtgcg acttcgcatc cttcgacacg gagatcacgc
     1261 tgtacaacaa cagcgggggt gcggcactgg gctacaacga cgatggttgc gccccctcct
     1321 cgatcgtgag ctggacagcc acattcaccg ggcaattgcg cgtgctggtg gacgcctggc
     1381 cgtgcggctc gatggcctct tgtgccgacc tgacgatcac atgtgcggcg gccggtggct
     1441 gccctgccgg cgaagtggcc gactgcaatg gcaactgtgt gccaacgagc tggttgggcg
     1501 acgggctctg cgatgacggc gttttcacgt acaacggggt gcccatctac ctcaactgcg
     1561 catcgctgaa ttttgacaat ggcgactgtg gtggtggagg ttgtgccgct ggcgagatcc
     1621 ccgattgcaa cggcaactgc gcccccgcga gctgggtggg ggacggtact tgcgacaatg
     1681 gtgcccggtc gtacaacgg
//
LOCUS       DAOQOC010000760         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53145_length_1697_cov_3.702801, whole genome shotgun sequence.
ACCESSION   DAOQOC010000760 DAOQOC010000000
VERSION     DAOQOC010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53145_length_1697_cov_3.702801"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PL106_11510"
     CDS             <1..504
                     /locus_tag="PL106_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA33739.1"
                     /translation="LYIIIKPFIRRERMAEDHLHDAVPDLHPPKAVPVRRVCVTVDFS
                     AMDQRSIDAAIAQGGIGAEYVLIHVTESAVARYSGTLSHDRETQGDARNLTRYVQHLQ
                     GLGYRATGRTGQGHAVSTIAAIVNSSDCDLLVMGAHGHRGLKDMLFGATVNAVRHRVK
                     VPVLIVR"
     gene            620..1066
                     /gene="smpB"
                     /locus_tag="PL106_11515"
     CDS             620..1066
                     /gene="smpB"
                     /locus_tag="PL106_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNA33740.1"
                     /translation="MPAAMPEVKNKRAAFEYFFLEQYECGLLLTGSEVKSLRNGDASI
                     AEAFCTFVGNELVVRNMTIQPFEKAKNYGHEPKRDRKLLLHRTELRKLQRKLKDQGVT
                     IVPVRVFFSEKGLAKLDIALAKGKKTYDKRDSIKERDVKRDMAREG"
     gene            1197..>1697
                     /locus_tag="PL106_11520"
     CDS             1197..>1697
                     /locus_tag="PL106_11520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA33741.1"
                     /translation="MTWRERVAESAQQCVPGGMQERIALGILLVLALVLRCWGFPHIP
                     YTHDEISALVRVDFPTLGDAISKGVWNIDTHPPGTHALLWCWTHVFGSSEGAVKLPFI
                     LMSVAALFLLYRFAHAWAGAGTAVIVTALLATLQYTVMYGQIARPYAMGLFTTALLAD
                     NMTRYLG"
BASE COUNT          350 a          529 c          527 g          291 t
ORIGIN      
        1 ttgtacatca tcatcaagcc cttcatccga cgcgaacgga tggccgagga tcacctgcat
       61 gatgccgtac cggacctgca tccgcccaaa gccgtacccg ttcggcgcgt ttgcgtcacg
      121 gtcgatttca gcgcgatgga ccagcgcagc attgatgccg ccatcgcgca gggggggatc
      181 ggagcggagt acgtgctgat ccatgtgacg gaatcggccg ttgcgcggta ctccggaacg
      241 ctgagccacg accgcgaaac acaaggcgat gcccggaacc tcacgcgtta tgtgcagcac
      301 ctgcaggggc tgggttaccg cgccacgggc cgcaccggtc agggccacgc cgtcagcacc
      361 atcgccgcga tcgtgaacag cagcgactgc gatctgctgg taatgggtgc gcacggccac
      421 cgcggcctga aagacatgct cttcggcgcc acggtgaatg cggtgcgcca cagggtgaag
      481 gtgccagtgc tgatcgtgcg ctgagagggt gatccagggc gatcgcaggg gccgccaccc
      541 ggtccatcgg aacagccacc ctgaacgttg taccggtcgc ctctgccctg ctcatccgca
      601 cattcatcat cttcgcccca tgcccgccgc catgcccgag gtgaagaaca aacgtgccgc
      661 gttcgagtac ttctttctcg agcagtacga atgcgggttg ctgctcaccg gtagcgaagt
      721 gaagagtctg cgcaatggcg atgccagcat cgccgaggcg ttctgcacct tcgtgggtaa
      781 cgaactggtg gtgcggaaca tgaccattca acccttcgag aaggcgaaga actacgggca
      841 cgagcccaag cgcgaccgga agctgctgct gcaccgtacc gagttgagga aattgcaacg
      901 caagctgaag gatcaagggg tgaccatcgt tccggtacgc gtgtttttca gtgagaaagg
      961 gctcgccaaa ctggacatcg cactggccaa gggcaagaaa acgtacgaca agcgcgacag
     1021 catcaaggaa cgcgacgtga aacgcgacat ggcacgggag ggatgatgcc atcgaccgac
     1081 atgccgacac gcgcacgtgg aactccggga gggaacctct cccctacgct ccatgaactg
     1141 gcggcagcac cttcgaaacc cctacgaaag gaaacatatc catgggatcc ggaaccatga
     1201 cctggcgtga acgtgttgcg gaaagtgcgc agcagtgcgt acccggcggc atgcaggagc
     1261 gcatcgccct gggcatactg ctggtgttgg ctctggtact gcggtgctgg ggattcccgc
     1321 acatccccta tacgcacgac gagatcagcg cattggtccg cgtggacttc cccaccctgg
     1381 gcgatgccat ctccaaaggc gtgtggaaca tcgacacgca ccctcccgga acgcacgcct
     1441 tgctgtggtg ctggacgcac gtcttcgggt ccagtgaagg agcggtcaag ctgcccttca
     1501 tcctgatgag cgtggccgcg ttgttcctgc tgtaccggtt cgcgcacgcg tgggccggtg
     1561 ccggtacggc ggtgatcgtg accgccctgc tggccaccct gcaatacaca gtgatgtacg
     1621 gacagatcgc ccggccgtac gccatgggcc tgttcaccac ggccctgctg gccgacaaca
     1681 tgaccagata cctggga
//
LOCUS       DAOQOC010000761         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53206_length_1696_cov_3.060938, whole genome shotgun sequence.
ACCESSION   DAOQOC010000761 DAOQOC010000000
VERSION     DAOQOC010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53206_length_1696_cov_3.060938"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1696)
                     /locus_tag="PL106_11525"
     CDS             complement(<1..>1696)
                     /locus_tag="PL106_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA33742.1"
                     /translation="QGASSAFTDGRIGYAYLGGPYLQLLYKANGWGTDQVNRVFGHET
                     AHIFHAFDEYAGSGTSNCARTFNGTQNANFQGAQCNGMAPCVMVGNTWTGSGATRQWD
                     LCAHTPAHLGWNSLLVPPTPKKPLNDTVLTTQPVMFKWQRNGAPPSAYGYLKVFDRSS
                     GDLVQCVYSGQNDSLPLNLVNGSYEWTISQGNTNDASGWAGVLGAAGQFKVNAPLNAS
                     FTRAPAVVCAGSYVDFTNTSTGAPVSWSWDFPGGQPAAYDGPDPPPVLYMIPGYKSVS
                     LTVSDGSTEHTFTQNNAVTVTGGQPLPFVQDMNGGGFPPPGWSATTTGQGLAWSGDAV
                     PGCGAQTSAFVNGWSYHGPAATARLSTPRIDLTQATLPYLKFRYSYAPKSPAATEMLQ
                     VYGHDCGQVNTATFFNRAGAALATNGGGFVSGQPWMPTTCEVWHEVALPVDALVGRIS
                     QFWFNLFSIGGQNVFVDDVTVFDGVRLPVRVLLQGPWDEEEQLMDDGLRGAGLVPDAE
                     PFTAAGYVFPDAGIGQMAAPGVLAVTGPQAIVDWVVLELRDAADPAQVLFVRPALLL"
BASE COUNT          314 a          544 c          526 g          312 t
ORIGIN      
        1 gtagcagcag tgccgggcgc acgaaaagca cttgggcggg gtcggccgcg tcgcgcaatt
       61 ccagcaccac ccaatcgacg atggcttgcg gtcctgtaac ggcaagtacc ccaggggcgg
      121 ccatctgacc gatccccgcg tcgggaaaaa cataccccgc cgccgtgaac ggctcggcat
      181 ccggcaccag acccgcaccg cgcaacccat cgtccatcag ttgctcttcc tcgtcccacg
      241 gcccttgtag cagcacgcgg accgggaggc gcacaccgtc gaagaccgtg acgtcatcca
      301 cgaaaacatt ctgcccgccg atactgaaca aattgaacca gaactggctg atgcgcccga
      361 ccagcgcgtc aaccggcagg gccacctcat gccagacttc gcatgtcgtt ggcatccagg
      421 gttgccctga tacgaacccg ccgccgttgg tggccaatgc ggcgcctgcc cggttgaaga
      481 aggtcgcggt gttgacctga ccgcagtcat ggccgtaaac ctggagcatt tcggtggcgg
      541 ccgggctctt gggcgcatag ctgtaccgga acttcaggta gggcagcgtg gcttgggtca
      601 ggtcgatccg aggcgtgctg agacgggcgg tggcggccgg cccatggtag gaccatccgt
      661 tcacgaacgc actggtctgc gccccacatc ccggaaccgc atcgccggac caggccagtc
      721 cttgccccgt ggtggtagcc gaccagccag gcggtgggaa gccgccaccg ttcatatcct
      781 gcacgaaggg aagtggctgg cctcccgtga cggtaaccgc gttgttctgg gtgaaggtgt
      841 gctcggtgga gccatcggaa acggtaagtg aaacactctt gtatccgggg atcatataga
      901 gcactggcgg tgggtcgggg ccatcgtacg ccgctggctg ccctcccggg aagtcccacg
      961 accacgacac gggtgctccc gtactggtgt tggtgaagtc gacatacgac ccggcgcaga
     1021 ccaccgcagg ggcccgtgtg aatgaggcgt tgagcggtgc gttcactttg aactgccccg
     1081 ctgcgcccag gacacctgcc caaccactgg catcattcgt gttgccctga ctgatggtcc
     1141 attcgtaact gccattcacc aggttgagcg gtagcgaatc attctgcccg gagtacacgc
     1201 attgcaccag atccccactg ctccgatcga agaccttgag gtagccgtac gcactgggcg
     1261 gggcaccgtt gcgttgccac ttgaacatga cgggctgggt ggtcagcacc gtgtcgttga
     1321 gcggtttttt tggtgtgggc gggaccaaca gcgaattcca tcccagatgc gcgggcgtat
     1381 gcgcgcacag atcccattgc cgcgtggcgc cactgcccgt ccacgtattc cctaccatca
     1441 cacaaggagc catcccgttg cattgggcgc cttggaagtt cgcgttctgc gtcccattga
     1501 acgtccgcgc acaattggat gtgccgctcc cggcatactc atcgaacgca tggaaaatgt
     1561 gcgcggtctc gtgcccgaac acgcggttca cctggtcggt gccccatccg ttggccttgt
     1621 acagcaactg gaggtacgga ccgcccaggt acgcgtatcc gatacgcccg tcggtgaagg
     1681 cggatgaggc gccttg
//
LOCUS       DAOQOC010000762         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53270_length_1695_cov_3.295732, whole genome shotgun sequence.
ACCESSION   DAOQOC010000762 DAOQOC010000000
VERSION     DAOQOC010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53270_length_1695_cov_3.295732"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PL106_11530"
     CDS             <1..190
                     /locus_tag="PL106_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33743.1"
                     /translation="DELRHDIKQHENHLEKFAMDHPVASDHVLFTDHQGLRDRIHTFE
                     KLYHELKVEFMRWLADRM"
     gene            complement(245..1084)
                     /locus_tag="PL106_11535"
     CDS             complement(245..1084)
                     /locus_tag="PL106_11535"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNA33744.1"
                     /translation="MKALRLALRALIQAAPFKGRYRLADKAGAALAGDLHEVFGLNGV
                     GVGIDHAVLTHRLMYYGLYEENVMNFLRRDLRPGDVVFDPGANIGYFAAVCLGLVGSK
                     GHVYSFEPSNAAHAHILKNNPSPPANWTLEHAALTDHSGEMTFYDTPRVMTRGFACLE
                     GTYEPKDRIPHQVKVFSLDDYCAARNINTIAFLKLDIEGSELKALQGAARMLASDSVR
                     TVLVETTLLDHTVEMTRTIDDLLRGYGYRSHRARRNGVPDPINVMEHLGLREDILWTR
                     TER"
     gene            complement(1138..>1695)
                     /locus_tag="PL106_11540"
     CDS             complement(1138..>1695)
                     /locus_tag="PL106_11540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA33745.1"
                     /translation="EDHILDVGGYPHTWTSAPQDVALIECLNLQVYPWEEEKDHPGHR
                     IRIVEGNGCDLKYADNSYPIVFSNSVIEHVGDFEAQQAFAREVRRVGRKLWIQTPAYG
                     CPIEPHYLTPFVHWYPKWFRRRTARYFTVWGLLEKPTKAEIDDMVDHTRLLTKAEMRT
                     LFPDCEIRTERLLGFIPKSYIAIRR"
BASE COUNT          305 a          525 c          525 g          340 t
ORIGIN      
        1 cgatgagctg cgacacgata tcaagcagca cgagaaccac ctggagaagt tcgccatgga
       61 ccacccggtg gcctccgatc atgttctctt caccgaccac cagggcctac gcgaccgcat
      121 acataccttc gagaaactct accacgaatt gaaggtggag ttcatgcgct ggttggcgga
      181 tcggatgtga gacaggtccg ctggacaagt tcgagggtgc gatcgatcgc gcccttgggc
      241 atgttcaccg ttcggtcctg gtccagagta tgtcctcgcg cagcccgagg tgttccatca
      301 cgttgatggg atccggcacg ccgttgcgcc gtgcccgatg ggaacggtag ccgtatccac
      361 gcagcaggtc atcgatcgtg cgtgtcatct ccaccgtgtg gtccagcagg gtggtctcca
      421 ccagcacggt gcgaaccgag tccgaggcca gcatccgtgc cgctccttgt agggccttca
      481 gctcgctccc ttcaatgtcc agtttcagga atgcaatggt atttatgtta cgtgcagcgc
      541 agtaatcatc cagcgagaac accttcacct ggtgcgggat ccgatccttc ggttcgtagg
      601 tcccttcgag gcaggcgaag cctcgggtca tcacccgggg cgtgtcgtag aaggtcatct
      661 cgccgctgtg atccgtgagg gccgcatgct cgagcgtcca gttggcggga ggggatggat
      721 tgttcttcag gatgtgcgca tgcgccgcgt tgctcggctc gaaggagtac acatgcccct
      781 tgctgcccac caacccgaga cacaccgcag cgaagtagcc gatgttcgct ccgggatcga
      841 agaccacgtc gcccggccgc agatcgcgac gcaggaaatt catcacgttc tcctcgtaga
      901 ggccgtagta catcaaccgg tgcgtgagca ccgcgtgatc gatccccacg cccacgccat
      961 tcaacccgaa cacttcgtgc aggtcgccgg ccagcgcggc ccccgcttta tcggccaacc
     1021 gataccggcc tttgaagggc gcggcctgga tcagtgcgcg gagcgcgagg cggagtgcct
     1081 tcataacggg tggcgaatct acccgctgga gcccggttct cgcgtgccgg gctccggtca
     1141 acgccgaatg gcgatgtagc tcttgggaat gaagccgagc aagcgttcgg tgcggatctc
     1201 gcagtccggg aacagggtgc gcatctcggc cttggtgagc agacgtgtgt ggtccaccat
     1261 gtcatcgatc tcggccttgg tgggcttctc cagcagcccc cataccgtaa agtaccgtgc
     1321 tgtacgcctg cggaaccatt tggggtacca atgcacgaag ggggtgaggt agtgcggctc
     1381 gatcgggcat ccgtacgcgg gcgtttggat ccacagtttc cggcccacac ggcggacctc
     1441 gcgcgcgaag gcttgttggg cctcaaagtc gcccacgtgc tcgatcaccg agttgctgaa
     1501 gacgatgggg tagctgttgt ccgcgtactt caggtcgcag ccattgccct cgacgatccg
     1561 gatgcggtgg cccggatggt ccttctcctc ctcccacggg tacacctgca ggttgaggca
     1621 ttcgatcaag gccacgtcct gcggtgcact cgtccaggtg tgcgggtagc cgcccacatc
     1681 cagaatgtgg tcctc
//
LOCUS       DAOQOC010000763         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53335_length_1694_cov_3.425869, whole genome shotgun sequence.
ACCESSION   DAOQOC010000763 DAOQOC010000000
VERSION     DAOQOC010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53335_length_1694_cov_3.425869"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..961)
                     /locus_tag="PL106_11545"
     CDS             complement(200..961)
                     /locus_tag="PL106_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33746.1"
                     /translation="MTPNADGANVIRDFSGVTLLLNAGTTTFMEPGATPYAADYPTSN
                     VAQEVVLPGGTSYSYFNLTTVPCDMLGEGIGGSDPTVYTDPKTPLQFPLSYPNYFIDY
                     YAIGGTDYGVSRAYMGNGAVILPTRTFNDVLKMASTSGAIDFFRSNPVSHLVKIDKDG
                     TVLVFESLASSFAQAATPAVLEVFPQPATDAVRVSGLTSSGTGQLFDIHGRSIERGNH
                     IPGVLDLDLAPLPSGPYTLWINAGTGRRSLRIIRQ"
     gene            1309..1524
                     /locus_tag="PL106_11550"
     CDS             1309..1524
                     /locus_tag="PL106_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33747.1"
                     /translation="MSELLQHLAFTFAQQVDLLGGFGRSGEAAGIRRAIPVLIGDPPM
                     AMRLTATTTSSGRESFSKYPVAPAHKA"
BASE COUNT          313 a          479 c          531 g          371 t
ORIGIN      
        1 gttcattgtc gatatatcca ttttgcccgc ggtcgcgatg cgcatgcgtt cgggttcatc
       61 gcataactgc ccccggagtt cccggtaggc aataccattt tcatgtgaaa tacccccgac
      121 cacctgttga tggaagaagg ggcacatgca cacctatcgg atgcgcatgt gcccctgcct
      181 atacagttcg agcgggccct tattgtcgaa tgatcctcag gctgcggcgg ccggtcccgg
      241 cgttgatcca caacgtgtat ggtccgctgg gcagcggggc gaggtccaga tcaagtacgc
      301 ccgggatgtg gttcccccgt tcgatcgatc ggccatgaat gtcgaatagc tgccctgtgc
      361 ccgacgaggt caggccggaa acgcgcaccg catctgtggc cggttgcggg aagacctcca
      421 gaacggccgg cgttgctgct tgtgcaaagc tcgatgccag tgattcgaac acgagcaccg
      481 tgccgtcctt atcgattttg accaggtgtg ataccgggtt gctgcggaag aagtcgatgg
      541 cgccgctggt gctcgccatt ttcaggacgt cgttgaacgt tcttgttggc aggatcaccg
      601 cgccattgcc catgtacgcg cgggagacgc cgtagtccgt tccgccaata gcgtaatagt
      661 cgatgaagta gttcgggtag gacaagggga actgcagcgg tgtctttgga tcggtgtaga
      721 cagtgggatc cgatccaccg attccttctc ccagcatgtc gcacggtacg gtggtgaggt
      781 tgaagtaaga gtagctcgtt ccgccgggca gaacgacctc ttgggccacg ttgctggtcg
      841 gatagtcagc ggcatacggt gtggcgcccg gctccatgaa cgttgtggtg ccggcgttca
      901 acaacaaggt cacgccgctg aagtcccgga tgacgttggc tccgtcggca ttgggggtca
      961 ctggtaccgg gatccgttac caagtgaacg gcgcaggagg cgcccaggag ctgcacattg
     1021 ccatgctcga tagcgggttg tgcagaggtg atcagaggcg cggaaagaag aaggagtgcg
     1081 gaatgtcgtt ccatgcgttg cggctgttgg gcaaggtccc atgaccttgg ggctttcgtc
     1141 aatgccactt tcatgtgggc atgtccaccg cgctccacgg ggatagagct tgttcagcgc
     1201 atcggtgcgc gagttcacct gcgatttgtc gtacacgttg cagatgtggg cgcgcaccgt
     1261 ttcgatgctc agccgcagcc gctcggcgat ctccttgtag cgataccctt gagcgaactg
     1321 ctccaacacc tggcgttcac gttcgcgcag caggtcgatc tccttggcgg ttttgggcgg
     1381 agcggtgaag cggccggaat acggcgtgcg atccctgtgc tcatcggtga tcctcccatg
     1441 gcgatgcgct taacggccac caccacctct tctggccggg aatccttcag caagtatccc
     1501 gtggcacccg cgcacaaggc gtgaacaggc tgtcgtcatc gtcctgtacc gtgcatacaa
     1561 gcacttgtgc cttgggccag cgggtcatca cccgtcacgt gcattatacc atttacgcat
     1621 tcaaggccgg ccaagagccg cgctgtgatc ttttcggagg acgatacgcg aaacaggttg
     1681 cgtagcgggg ccta
//
LOCUS       DAOQOC010000764         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53341_length_1694_cov_3.248322, whole genome shotgun sequence.
ACCESSION   DAOQOC010000764 DAOQOC010000000
VERSION     DAOQOC010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53341_length_1694_cov_3.248322"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1370)
                     /locus_tag="PL106_11555"
     CDS             complement(57..1370)
                     /locus_tag="PL106_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA33748.1"
                     /translation="MDLTEMIAALEQALALSPDNAPLRLQLAALCMNADRWSDAEMHY
                     KQVLINNPQDQKAKLGLARLFQRTDRLSAAIVIYDELYDRGVRDPELLLAYSRALVRD
                     GSDNRAQQVYMELVAKAPNMADDELDSLFRLSGREVGDIEDGDDNDALDRLLIKPDVK
                     FADVGGMDGVKKEIALKIIMPFRQPDLYKAYNKKAGGGILLYGPPGCGKTFIAKATAG
                     EIDARFVSVGISDVLDMWIGNSEKNLAGIFGAARENTPCVLFFDEIDALGANRGHMKH
                     SGGRHLINQFLSELDGTGSTNEGILIMGATNAPWHMDPAFRRPGRFDRIIFVPPPDQE
                     GRKRILDLLLRGMPADDVDTASIAKVTDGFSGADLKGLIDACVEDKLADAFSSGKPEP
                     ITTRDMRKAAGRLRPTTREWFNSAKNYALYANDGGLYDEILKYMK"
BASE COUNT          285 a          521 c          539 g          349 t
ORIGIN      
        1 gcgggcatgc cgcaacaaca gcagggcccg gcgcaggtgc gcgtcctcca tcacactcac
       61 ttcatgtatt tgagtatctc gtcgtacagc ccgccatcgt tggcgtacag ggcgtagttc
      121 ttcgcgctgt tgaaccattc acgggtcgtc ggcctcagcc tgcccgcggc cttcctcatg
      181 tcccgggtgg tgatcggttc gggcttgcca ctgctgaacg cgtcggcaag cttgtcctcc
      241 acgcacgcgt cgatcaggcc cttcaggtcg gcaccgctga agccgtccgt gaccttcgcg
      301 atggaagcgg tatcgacatc gtcggcgggc atgccgcgca gcagcagatc gaggatgcgc
      361 ttgcgccctt cctgatcagg tggcggtacg aagatgatgc ggtcgaagcg gcccgggcgg
      421 cggaaggcgg gatccatgtg ccagggcgca ttggtggcac ccatgatcag gatcccctcg
      481 ttggtgctgc ccgtgccatc gagctcgctg aggaactggt tgatcaggtg ccgaccaccg
      541 ctgtgcttca tgtggccgcg gttggcgccc agcgcgtcga tctcgtcgaa gaagagcacg
      601 cagggcgtgt tctcacgcgc cgcaccgaag atgcccgcga ggttcttctc actgttgccg
      661 atccacatgt ccagcacgtc gctgatgccc acgctcacga accgcgcatc gatctcgccc
      721 gctgtggcct tggcgatgaa ggtcttgccg catcccggcg gtccgtacag caggatgccg
      781 ccaccggcct tcttgttgta cgccttgtac aggtcgggct gcctgaaggg catgatgatc
      841 ttcagggcga tctccttctt cacgccatcc atgccgccca catcggcgaa cttcacatcg
      901 ggtttgatga gcaggcggtc cagcgcgtca ttgtcgtcgc catcctcaat atccccgacc
      961 tcgcgaccgc tcaggcggaa gaggctgtcc aactcatcat cggccatgtt cggtgctttg
     1021 gccaccagtt ccatgtacac ctgttgggcc cggttgtccg atccatcgcg taccagggcg
     1081 cgactgtacg cgagcaacaa ttccgggtcg cgcactccac ggtcgtacag ttcatcatag
     1141 atcacgatgg cggcactcaa gcggtcggta cgctggaaca gccgggccag gccgagtttg
     1201 gccttttggt cctgcgggtt gttgatgagc acctgcttgt agtgcatttc ggcatccgac
     1261 cagcgatcgg cgttcatgca cagggcggcc agttgcaagc gtaggggcgc attgtccggg
     1321 ctgagggcga gtgcctgctc caatgcggcg atcatctcgg taaggtccat ggtgccgaaa
     1381 agtagggcgg cgcgggacct gcatgatccg ctggactcct ttcaagcccg atcggtgtgt
     1441 cgaaatggcc ccgacaaacc tgcgcaacgg aacgaggggc gaatttcatg gtgaccagta
     1501 cgatcgcact gggtgcgtgt gcaaggctgt gcggtcctgg tccttgttca gttcgttgga
     1561 tcgggcccac cgggtcggct taaacttgcc gggaacaagt caaggatcgg gaagagggca
     1621 tgaaacggac catgggcgca catccgtgca agggagcgcc gctcagtggt cttgccgggc
     1681 tgggcttcgt ggtc
//
LOCUS       DAOQOC010000765         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53464_length_1692_cov_4.314600, whole genome shotgun sequence.
ACCESSION   DAOQOC010000765 DAOQOC010000000
VERSION     DAOQOC010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53464_length_1692_cov_4.314600"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PL106_11560"
     CDS             <1..255
                     /locus_tag="PL106_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33749.1"
                     /translation="PVAGTTAPGLMTYPSPSSGLITLEGTDGAPGLLRVQDITGRLLH
                     EERCTEWPCTIDLSAAPRGTHLVTFVRNDGMTRRTRIVLR"
     gene            complement(389..1333)
                     /locus_tag="PL106_11565"
     CDS             complement(389..1333)
                     /locus_tag="PL106_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33750.1"
                     /translation="MKNPIIPIAATVVALLTISACKKDEANGGILPPVGNSPLRDLFT
                     DHITQETQTFTVDATQGGTVTGANGTRVTFPPNGFRHANGTVVSGTVQVKLLEALKIG
                     DMIWINTQTVGDDNGTDRLLRSGGELRVTATQNDNELQLGPNGMSVMVPTENPDPLMA
                     LFTANAPRDSGMVWTPVDSSNLTVVSDTSLNTNFYWFTTDSLQWINCDYFYNYPATTS
                     LSATLPDGQPEDSTMVWIAFPSENAVMGLNYLTEQDLFTSWQVVPVGMQATVVGLYRN
                     GSQYYSAFSTVSITDMMSVPLTFSPTTLDQFQQDIDGI"
     gene            complement(1406..>1692)
                     /locus_tag="PL106_11570"
     CDS             complement(1406..>1692)
                     /locus_tag="PL106_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNA33751.1"
                     /translation="RQDPTMINTVFSLAAITYGPLLGLFAFGLFTRWSVQGAAVPLVC
                     ICAALGTYQLREHSAALFGGYHMGFETLILNGLLTFGGLWLVSRSPQDEV"
BASE COUNT          337 a          486 c          527 g          342 t
ORIGIN      
        1 ccggtggccg gaacgacagc accggggttg atgacgtatc cctccccctc cagtggcctc
       61 atcaccctgg aaggcaccga cggggcgccc gggctcctgc gggtgcagga catcacaggc
      121 aggctgctgc atgaggaacg atgcacggag tggccctgca cgatcgatct ttcagctgcc
      181 ccccgaggca ctcacttggt cacgtttgtt cgcaacgacg gtatgacccg acggacgcgt
      241 atcgtgctgc gttgagtttt cggaccgatg gagaggggat ctgacccggg accagaggaa
      301 gcaggcttac cgaccttctg gcgccaaatg gtcctgggtc agatagcccg cttcccgatc
      361 cttggaaccc agcaccgggc ccctgcactc aaatgccgtc gatgtcctgt tggaactggt
      421 ccaaggtggt aggtgagaag gtgagcggta cggacatcat atcggtgatg ctcaccgtgc
      481 tgaaggcgga gtagtattgt gacccgttgc gatagaggcc gaccacagtg gcctgcatgc
      541 ccaccggaac cacctgccag gaagtgaaaa gatcctgctc cgtgaggtag ttcaagccca
      601 tcaccgcgtt ctccgatggg aaggcgatcc agaccatcgt actgtcctct ggttgtccat
      661 caggaagggt cgccgagagg ctcgtggtcg cgggatagtt gtagaaatag tcgcagttga
      721 tccactgaag gctatccgtg gtgaaccagt agaagttggt gttcaacgag gtatccgaaa
      781 caaccgtcag gttggacgaa tccaccggcg tccacaccat gccactgtca cgcggagcgt
      841 tggccgtgaa cagggccatc aaagggtcgg ggttctcggt cggtaccatt acggacatgc
      901 cgttggggcc gagttggagt tcgttgtcgt tctgcgtggc cgttacccgg agttcaccac
      961 cactgcgaag aagcctgtcc gtgccgttat catcacccac ggtctgggta ttgatccaga
     1021 tcatgtcacc gatcttgagc gcctcgagga gcttcacctg caccgtgccg ctcaccacgg
     1081 taccgttcgc atggcggaat ccgttcggtg ggaaggtgac acgtgtgccg ttggcccctg
     1141 tcacggtgcc gccttgcgtg gcgtccacgg tgaatgtctg tgtttcctgt gtgatgtggt
     1201 cagtgaagag gtcgcgcagg gggctgttgc caaccggcgg caggatcccg ccgttggcct
     1261 cgtccttttt gcatgcgctg atggtgagca gtgcaaccac ggtggctgcg atggggatga
     1321 tggggttctt catgggttga tccgttgttc gtacccccaa agaccacaag acccatcaga
     1381 acgctgccca ggcaacgttg catcgctata cctcgtcttg gggacttctt gaaaccagcc
     1441 acagcccacc gaaggtgagc agtccattca agatgagggt ttcgaaaccc atgtgatagc
     1501 cgccgaagag cgccgcgctg tgttcgcgca attgataggt gcccaaggca gcgcagatgc
     1561 acaccagcgg tacagcagca ccttggaccg accaccgggt gaagagtccg aacgcgaaca
     1621 gccccagcaa cggcccatac gtgatggcgg ccagactgaa caccgtattg atcatggtgg
     1681 gatcctggcg gt
//
LOCUS       DAOQOC010000766         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53478_length_1692_cov_3.252291, whole genome shotgun sequence.
ACCESSION   DAOQOC010000766 DAOQOC010000000
VERSION     DAOQOC010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53478_length_1692_cov_3.252291"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PL106_11575"
     CDS             <1..872
                     /locus_tag="PL106_11575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA33752.1"
                     /translation="TEPNPQWPPRADVVWQQDDRYGLTKTGHMLASDEMTRVNLDFVD
                     DVCYAGLQHLEESIRDGKPAGLRELGPWKTVYEGLAHLPAAVRKSWFEFDNHYSDRSY
                     PAALPIVFRERPGTIMDVGANNGKWSLACLQHDPQVRMIAVDLPGQLRDLERNLTAAG
                     MSHRVDLRPTDMLDPTSTLPSGADVIWMSQFLDCFGEDEVVSILRKAHTAMAPHSALY
                     IMETFINRQRFEAGAFSLAATSLYFTAMANGNSRMYRAEHFEPLVEQAGLRITERIDD
                     LGLGHSLLRCVRP"
     gene            complement(873..>1692)
                     /locus_tag="PL106_11580"
     CDS             complement(873..>1692)
                     /locus_tag="PL106_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="all-trans-retinol 13,14-reductase"
                     /protein_id="HNA33753.1"
                     /translation="GAVIHARKRVTGLDMDGNGVRAVRTADGDVFSCRWCIANIHPAM
                     LLEMIPPGHIRPAYRTRIRSLDNTVSTFILHLAMKPGSFPYLNRNIYKFLVPDVWGAV
                     DAADAAPSAYMFSTPATSRGGEHADAITMMTYMRYSACAPWAGTHRTVAEPGERGPAY
                     EDFKHAHQERLLDVLEERFPGLRRGVTGVHSTTPLTFRDFIGTPDGNMYGIRKDSSSP
                     LRTFLSPRTKVPNLLLTGQNLNLHGLLGVTVGSVSTCAELVGKSHLLGRIHAAN"
BASE COUNT          322 a          506 c          574 g          290 t
ORIGIN      
        1 tgaccgagcc gaatccgcag tggccgccaa gggcggatgt ggtatggcaa caggatgatc
       61 gctacgggct caccaagacc ggtcacatgc tggcctcgga cgagatgacc cgggtgaacc
      121 tggatttcgt ggatgacgtg tgctatgcgg gacttcagca cctggaggag tccatccgcg
      181 atgggaagcc ggccggactg cgcgaactgg gtccctggaa aacggtatat gaagggctgg
      241 cccaccttcc cgcagcggtg cgcaagagct ggttcgagtt cgacaaccac tactcggacc
      301 gctcctatcc ggcggcattg cccatcgtgt tccgcgagcg gcccggcacc atcatggacg
      361 tgggcgccaa caacgggaag tggagcctgg cctgccttca acacgatccg caggtacgga
      421 tgatcgccgt ggacctgcca gggcaactcc gcgacctgga acgcaacctc accgcggcgg
      481 gcatgtccca tcgcgtggac ctgcgcccca cggacatgct cgatccaacg agcacgctgc
      541 cttccggtgc cgacgtgatc tggatgagcc agttcctgga ttgtttcggg gaggacgaag
      601 tggtgagcat cctgcggaaa gcgcatacgg ccatggcacc gcacagtgcg ctctacatca
      661 tggaaacctt catcaatcgg caacgcttcg aggcgggagc gttctccctt gcggccacct
      721 cgctctattt cacggccatg gccaacggca acagccggat gtacagggcc gagcatttcg
      781 agccgttggt ggaacaggcc gggctgcgca tcaccgagcg catcgacgac ctggggctgg
      841 ggcacagcct gctgcgctgc gtccggccgt gatcagttcg cggcgtggat gcgccccaac
      901 aaatggctct ttcccacgag ttcggcgcag gttgataccg aaccaacggt aacacccagc
      961 aggccgtgca ggttgaggtt ctgccctgtg agaagcagat tcggcacttt ggtgcgcggt
     1021 gagaggaagg tgcgcagggg cgatgaactg tccttgcgga tgccgtacat attgccgtcg
     1081 ggcgtaccga tgaaatcgcg gaaggtgagc ggtgtggtgc tgtgaacacc ggtcacaccg
     1141 cgccgcaggc cggggaaacg ctcctccagt acgtcgagca accgctcctg gtgcgcgtgt
     1201 ttgaagtcct cgtacgccgg cccgcgctca ccgggctcgg caacggtccg atgcgtgccg
     1261 gcccacgggg cgcatgcgga atagcgcatg tacgtcatca tggtgatggc atcggcatgt
     1321 tcgccaccgc gcgacgtggc cggggtggag aacatgtagg ccgagggcgc ggcatcggcg
     1381 gcatccacgg ctccccacac atcgggcacc aggaatttgt agatgttgcg attgaggtag
     1441 ggaaaggagc ccggcttcat ggccaggtga aggatgaagg tggacacggt gttgtccagc
     1501 gagcggatac gggtgcggta agcgggacgg atgtggcccg gagggatcat ttccagcaac
     1561 atggccgggt ggatgttggc gatgcaccag cggcaggaga agacatcccc atcggccgtg
     1621 cgtaccgcgc gcacgccgtt gccatccatg tccagaccgg tgacgcgctt gcgcgcgtgg
     1681 atcacggcac cg
//
LOCUS       DAOQOC010000767         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53484_length_1692_cov_3.122175, whole genome shotgun sequence.
ACCESSION   DAOQOC010000767 DAOQOC010000000
VERSION     DAOQOC010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53484_length_1692_cov_3.122175"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PL106_11585"
     CDS             <1..347
                     /locus_tag="PL106_11585"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNA33754.1"
                     /translation="NGAVQERDFFQDVMDVVRQIPKGRVTSYGAIARYIGAARSARIV
                     GYCMNNSHASLPPVPAHRVVNSSGLLTGKHHFATPTRMENLLKKEGVKVKDDQVVDFK
                     KKFWDPLRELDL"
     gene            406..834
                     /locus_tag="PL106_11590"
     CDS             406..834
                     /locus_tag="PL106_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33755.1"
                     /translation="MPHHHMITIRNDGTVRIELDLEHGKVTSTWSHSIRSEEYRLALW
                     HIHDLVRDHHLVLWLSDTRAMGAILHADEKWSIEVFMPEVIKLGLRRVAVVRSEDYFA
                     HTATARMVDATAVVAPFKVELFADAGDAEDWLLKEQAAFV"
     gene            886..>1692
                     /locus_tag="PL106_11595"
     CDS             886..>1692
                     /locus_tag="PL106_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNA33756.1"
                     /translation="MPITEAAIETALARIIEPDLKKDIVELDLVREVVVDENTVHVTV
                     EVSNPALHSRKRMEEAVSFQLKKELGNDVQVRVTVNALSGDRANVRKVLPKVKHIIAI
                     ASGKGGVGKSTITANLAAGLAQRGFSVGLVDADIYGPSMPLMFDVLHERPNVKKEPPS
                     PGASAAEGKNLIVPVESHGVKLLSIGFFADPSQAIAWRGPMASKALDQLFKDADWGEL
                     DVMLVDLPPGTGDIHLSLVQAIPLTGAIIVSTPQPVAVADARKGVGFFQLP"
BASE COUNT          344 a          519 c          528 g          301 t
ORIGIN      
        1 tgaacggtgc cgtccaggag cgcgacttct tccaggatgt gatggacgtg gtacgtcaga
       61 taccgaaagg gcgggtgacg agctacgggg ccatcgcccg gtacatcggt gccgcgcgtt
      121 ctgcgcgcat cgtgggctat tgcatgaaca acagccatgc ttcgttgcct cccgtaccgg
      181 cgcatcgcgt ggtgaacagc agcggcctgc tcaccggcaa gcaccacttc gccacgccga
      241 cacgcatgga gaacctgctg aagaaggaag gcgtgaaggt gaaggacgac caggtggtgg
      301 acttcaagaa gaagttctgg gatcccttga gggaactgga cctgtgaccg gaccggcagg
      361 ccgaccatac ccctatggat catcctgttg ccgaactttg aggtgatgcc ccaccaccac
      421 atgatcacca tccggaacga tgggaccgtt cgcatcgaac tcgacctcga gcatggcaag
      481 gtcacaagta catggtccca ctccatccgg agcgaagagt accggcttgc gctctggcac
      541 atccacgacc ttgtccgtga tcatcatctg gtgctctggt tgtcggacac gcgcgccatg
      601 ggcgccattc tccatgccga tgagaagtgg tccatcgagg tcttcatgcc ggaggtcatc
      661 aagctcggct tgcgacgcgt ggccgtggtg cgtagcgagg actatttcgc gcatacggca
      721 acggcccgca tggttgatgc cacggccgtg gtcgcaccct tcaaggtgga actcttcgct
      781 gatgccggtg atgccgaaga ctggctgctc aaggaacagg ccgcattcgt ttaaggttca
      841 ttccccaggt cgcgcaggac ctaatttcgc ggcgccatac gaaccatgcc gatcactgaa
      901 gccgccatcg agaccgcgct cgcgcgcatc atcgaacctg acctgaagaa agacatcgtg
      961 gaactcgacc tcgtgcgcga ggtggtcgtg gatgagaaca ccgttcacgt gaccgtggag
     1021 gtgagcaacc ccgccctgca cagccgcaag cgcatggagg aggcagtgag cttccaactg
     1081 aagaaggaac tgggcaacga tgtgcaggtg cgtgtcacgg tgaatgcctt gagtggcgac
     1141 cgcgccaacg tgcgcaaagt gctgcccaag gtgaaacaca tcatcgccat cgccagcggc
     1201 aaaggcggcg tgggcaagag cacgatcacc gccaacctcg cggcagggct ggcccaacgt
     1261 ggtttcagcg tggggctggt ggacgccgac atctacggcc ccagcatgcc gctgatgttc
     1321 gatgtgctgc acgaacgccc gaacgtgaag aaggagccgc cttcgcctgg agcttcggcg
     1381 gccgagggaa agaatctcat cgtcccggtc gagagccatg gcgtgaagct gttgagcatc
     1441 ggcttcttcg ccgatccgtc ccaagccatc gcctggcggg gccccatggc gagcaaggcg
     1501 ctggaccagt tgttcaagga cgccgactgg ggcgaactcg acgtgatgct cgtggacctt
     1561 cccccgggca ccggcgacat ccacctctcc ttggtccagg ccattcctct gacgggagcc
     1621 atcatcgtga gcaccccgca acccgtggcc gtggccgatg cgcgcaaggg cgtcggattc
     1681 ttccaattgc cg
//
LOCUS       DAOQOC010000768         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53498_length_1692_cov_2.734270, whole genome shotgun sequence.
ACCESSION   DAOQOC010000768 DAOQOC010000000
VERSION     DAOQOC010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53498_length_1692_cov_2.734270"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PL106_11600"
     CDS             complement(<1..1488)
                     /locus_tag="PL106_11600"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNA33757.1"
                     /translation="MGKWIKRIFLGIGILLLLVLLAAVLIPILFKDRIEAAMKEQVNA
                     SLNATVDWGEWDLSILSSFPNASFSISDVKVCNRAPFEGICLARIGELQATVGVMSLF
                     GDRILIKRVAIIRPDVHAKVLPDGRANWDIALADSAATETPADTSATLFNVKLSEYSI
                     TDGHLIYDDASLPMLMDLAGLDHTGKGDFTQDLFVLSTNTHADSATVVFDGIKYLKNV
                     TADIKADLDMDMPNMKFTFKENEATINQLVLGFDGWVAMPTDDIDMDLKWDLKKTDFG
                     TLLSLVPAEFASDLKGVDMTGKAAFNGYVKGTYNDNTMPGFGLHIGVDNGRFKYPDLP
                     ASCENIFVDCGIVSPQGKDLDGMVVDLKRFALKLAGNPVEARMLLKTPMSDPDVDAVL
                     KADVDLANVKKVVPLGKDELNGALKADVTMVGRMSDVEAQGYEKFKAEGTLQLKGMAY
                     RSDSLPYGIGISELLFRFSPKFLELAKYDGTVGGSDLHASGRIDNY"
     gene            complement(1530..>1692)
                     /locus_tag="PL106_11605"
     CDS             complement(1530..>1692)
                     /locus_tag="PL106_11605"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNA33758.1"
                     /translation="SVPVKGRGRHDPCVVPRAVPIVEAMTCLVLADHLLRHRTSRVGP
                     PVVIGAVEG"
BASE COUNT          300 a          501 c          540 g          351 t
ORIGIN      
        1 gtagttgtcg atgcggccac tggcatgcag gtcgctgccg cccacggtac cgtcgtactt
       61 cgccagttcc aggaacttcg ggctgaagcg gaagaggagc tcgctgatcc cgataccgta
      121 cggcagggag tcgctgcggt aggccatgcc cttcagttgc aaggtgccct cggccttgaa
      181 tttctcgtag ccctgggcct ccacgtcgct catgcggccg accatggtca catcggcctt
      241 caatgcgccg ttcaactcgt ccttgccaag gggcaccacc ttcttcacat tggccaggtc
      301 cacatcggcc ttcagcacgg cgtccacatc gggatcgctc atgggggtct tcagcagcat
      361 gcgggcctcc accgggttgc cggccagctt cagggcgaat cgcttcaggt ccaccaccat
      421 gccgtcgagg tccttgccct ggggagaaac gataccacaa tcaacaaaaa tgttctcgca
      481 gctcgcgggc aggtcggggt acttgaaccg gccgttgtcc acaccgatgt ggaggccgaa
      541 accgggcatg gtgttgtcgt tgtaggttcc cttcacgtag ccgttgaaag cggccttgcc
      601 ggtcatgtcc acgcctttga gatcgctggc gaactccgcg ggcaccaggg agagaagcgt
      661 gccgaagtcc gtcttcttca gatcccactt gaggtccatg tcgatatcgt cggtgggcat
      721 ggccacccaa ccatcgaagc cgagaacgag ctggttgatg gtggcctcgt tctccttgaa
      781 ggtgaacttc atgttgggca tgtccatgtc caggtcggcc ttgatgtcgg cggtgacgtt
      841 cttgaggtat ttgatcccgt cgaagaccac ggtggcactg tcggcgtgcg tattggtgct
      901 cagcacgaag aggtcctggg tgaagtcgcc cttcccggtg tggtccagtc ccgcgaggtc
      961 catgagcatg ggcagggagg catcgtcgta gatcagatgg ccgtcggtga tggagtactc
     1021 gctcaatttc acgttgaaga gcgtggcgga ggtgtcggcc ggggtttccg tcgccgcact
     1081 atcggccagg gcgatgtccc agttggcgcg gccatcgggc agcaccttgg catgcacatc
     1141 cggccggatg atggccacgc gcttgatgag gatgcggtcg ccgaagaggc tcatcacgcc
     1201 cacggtggcc tgcaactcgc cgatgcgggc caggcagatg ccttcgaagg gtgcgcggtt
     1261 gcacaccttc acatcggaga tggagaagct cgcattggga aaactgctga ggatggacag
     1321 gtcccactcg ccccagtcca ccgtggcgtt aaggctggca ttcacctgtt ccttcatcgc
     1381 tgcttcgatc cggtccttga aaaggatggg gatgagcacc gcggccagga ggaccagcag
     1441 aagaagaata ccgatgccga ggaagatccg tttgatccac ttgcccatga tgcgctgttg
     1501 ttgttgtccg ttctggttgt tcgttccggc tatccttcca cggccccgat gacgacgggc
     1561 gggcccaccc tgctggtgcg atgcctgagg agatgatcgg cgagtaccag gcaggtcatg
     1621 gcttccacga tggggaccgc gcgcggcacc acgcaagggt cgtggcgacc ccggcctttg
     1681 acgggtaccg ac
//
LOCUS       DAOQOC010000769         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53537_length_1691_cov_3.946210, whole genome shotgun sequence.
ACCESSION   DAOQOC010000769 DAOQOC010000000
VERSION     DAOQOC010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53537_length_1691_cov_3.946210"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PL106_11610"
     CDS             <1..756
                     /locus_tag="PL106_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA33759.1"
                     /translation="SMPLAVLIDEGSASASEIVAGAIQDNDRGIIVGRRSFGKGLVQE
                     HMDLPDHSAVRITTARYYTPSGRSIQRPYGEGIDYDDDLSERFDHGEFLSPDSIHVDS
                     SLRYTTLNGRTVFGGGGIMPDLFVPADTAERSAYLTELLFTGAMNQFAFELAEQERER
                     LEAMGSPDRFNREFALGPATLDRFQTEAEKLGVRRDPNGFARSSRLIATRIKALVARN
                     IWKEPGFYRIALGDDGIYKAARTALDQATTEKR"
     gene            complement(822..1079)
                     /locus_tag="PL106_11615"
     CDS             complement(822..1079)
                     /locus_tag="PL106_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33760.1"
                     /translation="MTKKFALLATVAALFVACASGPTEEELKAKAQATEDSIKAAAQK
                     IEDDAKAAQAKLDSAAKAMADTVSTTVTGAVDAAKQAVTGK"
     gene            1206..>1691
                     /locus_tag="PL106_11620"
     CDS             1206..>1691
                     /locus_tag="PL106_11620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNA33761.1"
                     /translation="MPFQLPPLPYASNALEPYIDARTMEIHHGKHHNAYVTNLNKAIE
                     GTPLDGKSIEEILRGLDMKNAAVRNNGGGHFNHSLFWTVMGPNAGGAPSGELAEAIGS
                     AFGGFDAFKEKFAAAGATRFGSGWAWLCAHKGGKLEVCSTPNQDNPLMPDTGCGGTPI
                     LG"
BASE COUNT          327 a          541 c          506 g          317 t
ORIGIN      
        1 agcatgccgc tcgctgtact gatcgacgag ggttcggcca gcgccagcga gatcgtggcg
       61 ggcgccatcc aggacaacga tcgcggcatc atcgttggcc ggcgctcgtt cggcaaggga
      121 ctggtgcagg aacacatgga cctgcccgac cacagcgcgg tgcggatcac tacagcaagg
      181 tattataccc cgagcgggcg atccatccaa cggccctacg gcgaggggat cgactacgac
      241 gacgacctga gtgaacgctt tgaccatggc gagttcctca gcccggacag catccatgtc
      301 gactccagcc tgcgctacac cacgctcaat ggtcggaccg tcttcggtgg tggtggtatc
      361 atgcccgacc tcttcgtacc cgctgacacg gccgagcgtt ccgcctacct caccgaactg
      421 ctcttcaccg gcgccatgaa ccagttcgcc ttcgaattgg ccgaacagga acgcgaacga
      481 ctggaggcga tgggatcccc cgatcggttc aaccgggaat tcgcattggg cccagcaaca
      541 ctcgaccggt tccagaccga ggcggagaaa ctcggggtac gacgtgaccc caacggcttc
      601 gcacgttcga gccgactgat cgccacgcgg atcaaggcac tggtcgcccg gaacatctgg
      661 aaagagccag gcttctatcg gatcgccctc ggggacgatg ggatctacaa agcggcccgt
      721 acggcactgg atcaagccac cacggagaaa cgctgatcaa ccggcctgaa aagcgaaggg
      781 gccttgcggc cccttgctga tcgtgttctt tggaacggca attacttgcc ggtcacagcc
      841 tgcttcgcag cgtcaacagc accggtcacg gtggtgctca ccgtgtcggc catcgccttg
      901 gcggcgctgt ccaacttggc ctgggcggct ttcgcgtcgt cctcgatctt ctgggcggca
      961 gccttgatgc tgtcctccgt ggcctgggcc ttcgccttca actcctcttc cgtcgggccg
     1021 ctggcgcagg ccacgaacag tgctgccaca gtggcgagca gtgcgaactt cttggtcatt
     1081 ttacgtgcgt tttttggtga aagtgggcgc gaaagtagca tattccccca atggccgcac
     1141 acctccctcc actatgaggc tatctttcgc cgcgtcgcct ctttttggcg ccaaaaccga
     1201 ccgaaatgcc cttccaactc cctcccctgc cctacgcgtc gaacgcgttg gagccctata
     1261 tcgatgcccg caccatggag atccaccacg gcaagcatca taacgcatac gtgaccaacc
     1321 tgaacaaggc gattgaagga acacccttgg acggcaagtc cattgaggag atcttgcgcg
     1381 gactggacat gaagaatgct gccgtgcgga acaacggggg aggccatttc aaccacagcc
     1441 tcttctggac cgtgatgggc ccgaatgcag gaggggcgcc gtccggcgag ctcgccgaag
     1501 cgatcggttc cgcttttggt ggcttcgatg cgttcaagga gaagttcgcc gctgcgggcg
     1561 ccacccgttt tggtagcggc tgggcctggc tctgtgcgca caagggtggg aagctggagg
     1621 tctgctccac cccgaaccag gacaatccat tgatgcccga cacgggctgc gggggtacgc
     1681 ccatcctggg g
//
LOCUS       DAOQOC010000770         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_53571_length_1691_cov_2.536675, whole genome shotgun sequence.
ACCESSION   DAOQOC010000770 DAOQOC010000000
VERSION     DAOQOC010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53571_length_1691_cov_2.536675"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PL106_11625"
     CDS             <1..1329
                     /locus_tag="PL106_11625"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNA33762.1"
                     /translation="WYDRDLDEHVPREYLREIYGVSRSMSDAYDGIGPKYMRSLNYHA
                     AHDIGHALQDLAVVGCTSFAAKGGRTDDGHLLVGRNFDFWMGDDFSREKLVTFIRPTE
                     GIPHVLVGWGGFMGAVSGMNLEGLTVTINAARSALPFGARAPISIVTRDILQHAATID
                     EAVAIAKKHEVFVSESILVASARDGRAVIIEKAPDGMGVFDPGGDLVTCANHYQSERF
                     ANSAANQANIRESDSMARFRRMDQLVDSAGTIDPVKAAAILRDRYGMNGAPIGLGNPM
                     AINQMIAHHGVIMRPADRRIWVSAWPYQMGAFVCYDLRDVFARCAMDSISGPIHDPSR
                     TIAADPYLSTAAFAEQQWWQEQRTRIALAGMTGKTIAFTPAEEARFLAAAPDSYITRI
                     ALGDLHRLRGGMAEAADHYRAALALPLPSLQEHTRLEERLRECDQTTTAP"
     gene            1326..>1691
                     /locus_tag="PL106_11630"
     CDS             1326..>1691
                     /locus_tag="PL106_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33763.1"
                     /translation="MTSIRTPNAAEALERSLAHVDDLRSRSPWYRERLAKVGPITSWD
                     DFRNLPFTTKRELSEHHDRFRCMPHNAIAEHVSTSGTTGRAVSFVLSGADVDRLARNE
                     RYALGLAGITASDTVMITTT"
BASE COUNT          312 a          572 c          536 g          271 t
ORIGIN      
        1 tggtacgacc gcgacctgga cgagcacgtg ccacgggaat acctccgcga gatctatggt
       61 gtgagccgct caatgagcga tgcgtacgac ggcatcggcc ccaagtacat gcgatcgctg
      121 aactaccacg cggcgcatga catcggtcac gccctgcagg acctggcggt ggtgggctgc
      181 acctcgttcg cggccaaggg tgggcgtacc gatgacgggc acttgctcgt ggggcgcaat
      241 ttcgatttct ggatgggcga tgatttctcc cgggaaaagc tggtcacctt catccggccc
      301 acggagggca tcccgcacgt cctggtgggc tggggcggtt tcatgggcgc ggtgagcggc
      361 atgaacctcg aggggctcac cgtcaccatc aacgccgcgc gcagcgcgct tccgttcggg
      421 gcacgtgcac cgatctcgat cgtcactcgc gacatcctgc agcacgcggc caccatcgat
      481 gaggccgtgg ccatcgccaa aaaacacgag gtcttcgtga gtgagagcat cctggtggcc
      541 agtgcgcgtg atgggcgggc tgtcatcatc gagaaggcac cggacggcat gggcgtgttc
      601 gaccccggtg gtgacctggt gacctgcgcc aatcattacc agagcgagcg cttcgccaac
      661 agcgcggcca accaggccaa catccgcgag agcgacagca tggcgcgctt ccgccggatg
      721 gaccagctcg tcgattccgc cggcacgatc gacccggtaa aagccgccgc catactgcgc
      781 gaccggtacg gcatgaacgg tgcgcccatc ggcctgggca acccgatggc catcaaccag
      841 atgatcgcgc accacggcgt gatcatgcgc cccgccgacc ggcgcatctg ggtgagcgcc
      901 tggccgtacc agatgggcgc cttcgtgtgt tacgacctcc gcgatgtctt cgcccggtgc
      961 gccatggaca gtatcagcgg tcccatccat gatccttcac gaacgatagc tgcggacccg
     1021 tacctgtcca cggctgcgtt cgctgagcag cagtggtggc aggagcagcg cacgcgcatc
     1081 gcactggccg gcatgaccgg taagaccatc gcgttcaccc cggccgaaga ggcgcgcttc
     1141 ctggccgccg ctccggacag ctacatcacg cggattgcgt tgggcgatct gcaccggctg
     1201 cggggtggga tggcggaagc cgccgatcac taccgggccg cattggcatt gccactgccc
     1261 tccctgcaag agcatacacg gttggaggag cgcctgcgcg aatgcgacca gaccaccacc
     1321 gcaccatgac ctccatccgc accccgaacg ccgcagaggc actggaacga tcgctcgcgc
     1381 acgtggacga cctgcgctcg cgttccccat ggtaccgtga acggctggcg aaggtcggcc
     1441 cgatcacctc ctgggatgac ttccggaacc tgcccttcac cacgaagcgc gagttgagcg
     1501 aacaccacga ccggttccgc tgcatgccgc acaacgccat cgccgagcac gtctccacca
     1561 gtggaactac aggacgagca gtatcttttg tgctgagcgg cgccgacgtg gaccggctgg
     1621 cccgcaacga gcgctacgcg ctgggtctgg cgggcatcac ggcatcggac acggtcatga
     1681 tcaccaccac c
//
LOCUS       DAOQOC010000771         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54006_length_1685_cov_2.834356, whole genome shotgun sequence.
ACCESSION   DAOQOC010000771 DAOQOC010000000
VERSION     DAOQOC010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54006_length_1685_cov_2.834356"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1685)
                     /locus_tag="PL106_11635"
     CDS             complement(<1..>1685)
                     /locus_tag="PL106_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33764.1"
                     /translation="QSFCSVFTSATLAGSTVTFPGQGTWTVVSGAGVFSDEHDPATTV
                     TGAVIGENIYAWTVDNGPCSDPITSDQVSIFVYDANNPDADAGPGQELCTPSESTTMQ
                     GSTVTFPAQGTWTLVSGAGVFVDPNSPLAVVTGLAIGDNVFEWTVNNGPCANGTTTDQ
                     VTITVFDAGSANADAGPDQDLCSTSPNTVMAGSVVNYPAEGTWTLIDGAGTIADVNDP
                     ASAITGLAVGENIFQWTVSNGPCENANTMDQVSIFVYDEDNAAADAGPDQDRCTPATT
                     TGLAGSPVTFPATGTWTVVSGPGMVVNANDPASAVVDLGVGVNVLVWTVDNGPCANGL
                     TTDTVRINLFDDANPVADAGPDQDLCTPASSAQLAGSALIIPAQGTWTILQGTGTFSD
                     VNDPQATINGLSVGETIIRWSVDNGPCANGLTADTLSIFLYDDGQAAADAGPDQQLCT
                     PTTSTNLSGSAVTFPGTGTWSLLNGQGAIADPNDPNTLVTDLAVGENVFQWVVTNGPC
                     SEPLTTDEVSIFVFDHEAPIADAGPDKQICTPIDSTVLSGSALVFPATGTWTI"
BASE COUNT          299 a          528 c          526 g          332 t
ORIGIN      
        1 gatcgtccag gtgcccgtgg ccgggaaaac gagcgcgctg cccgaaagga cggtgctgtc
       61 gatcggcgtg cagatctgct tgtcggggcc agcgtcggca atgggggctt cgtgatcgaa
      121 cacgaagatg ctcacttcat cggtggtcaa aggctccgaa caggggccat tggtcaccac
      181 ccattggaac acgttctcac ccaccgcgag gtccgttacc aaagtattag gatcattcgg
      241 gtcggcaatc gcaccctggc cgttcagcag gctccacgta ccggtcccgg ggaaggtgac
      301 cgcactgccc gagaggttgg tcgatgtggt cggggtacag agctgctgat ccggaccggc
      361 atccgccgcc gcctgcccat catcgtacag gaaaatgctc aaggtatccg cggtgaggcc
      421 attggcgcat gggccgttgt cgaccgacca cctgatgatc gtctcaccca ccgaaagccc
      481 attgatggtg gcttgtggat cgttcacgtc agagaaggta ccggtgccct gcaggatggt
      541 ccacgtgcct tgcgccggta tgatcaatgc gctgcccgcc agttgcgccg aggatgccgg
      601 ggtgcacagg tcctggtcgg gtcccgcatc ggcaacgggg ttcgcatcat cgaagaggtt
      661 gatccgtacg gtatccgtgg tcagcccgtt cgcgcacgga ccgttgtcca cggtccaaac
      721 gaggacattc acgccaaccc cgaggtcaac gaccgctgat gccggatcgt tcgcattgac
      781 caccatcccg gggcccgata cgaccgtcca ggtacctgtt gccgggaacg tgaccgggct
      841 tcccgcgagc ccagtggttg ttgcgggagt gcagcgatcc tggtccggtc cggcatcggc
      901 ggccgcattg tcctcgtcgt acacgaagat gctgacctgg tccatggtat tcgcgttctc
      961 acacgggcca ttgctcaccg tccattggaa gatgttctcc ccgacggcca gtccggtgat
     1021 ggccgaggcc ggatcgttga catccgcgat cgtgcctgct ccgtcgatga gtgtccacgt
     1081 gccctcggct ggatagttga caacactgcc ggccatgacg gtgttcgggc tcgtggagca
     1141 cagatcctga tcgggacccg catccgcatt ggcgctgccc gcatcgaaga ccgtgatcgt
     1201 cacctggtcc gtggtggtac cgttggcaca tggtccgttg ttcacggtcc attcgaacac
     1261 gttgtccccg atggccaaac cggtcactac cgcgagcgga ctgttgggat ccacgaacac
     1321 accagcgccg ctcaccaggg tccacgtgcc ttgtgccggg aaggtcaccg tgcttccctg
     1381 catggtggtc gattccgacg gtgtacacaa ctcctggccc ggtcccgcat cggcatccgg
     1441 gttgttcgcg tcataaacga agatgctcac ctgatcgctc gtgatcgggt cggaacaggg
     1501 gccattgtcc accgtccatg cgtagatgtt ctcgccgatg acggcgcctg ttaccgtggt
     1561 ggccggatcg tgctcatcgg agaagacccc ggcgcccgat accacggtcc aagtaccctg
     1621 ccccgggaaa gtgaccgtgc ttcccgcgag ggtggcactg gtgaataccg aacagaacga
     1681 ctggt
//
LOCUS       DAOQOC010000772         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54327_length_1680_cov_3.780923, whole genome shotgun sequence.
ACCESSION   DAOQOC010000772 DAOQOC010000000
VERSION     DAOQOC010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54327_length_1680_cov_3.780923"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1313
                     /locus_tag="PL106_11640"
     CDS             309..1313
                     /locus_tag="PL106_11640"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33765.1"
                     /translation="MIIRTSFALAFLTGPVLGIIAQPHSAINQKDAQGRKQGPWQRTW
                     ADSDQIRYAGQFKDDKPVGNFSYFSTAGKLESILAWYPDGKAAHAKHYHPDGKLMAEG
                     RYIGEQKDSTWNYYDVNGALRSTEHWKNGKYHGEQEAYFANGKVAERCVWKDGERNGE
                     CQQFFENGQVRTSTTYVNGEANGPMRVLAEDGKPEIEGQYAKDLRSGLWKHYNTDGSI
                     LMQTLYEADKVVKEKKENGLFRTFYPDDQLMKEETYKHGKRHGTFTEYHDNGRWTMRP
                     TKVGPDGAEKNDTERVLEGQTKKREGQYKNDLQEGEWKEWDEKGKLIGTTVYKAGVAQ
                     "
     gene            1327..>1680
                     /locus_tag="PL106_11645"
     CDS             1327..>1680
                     /locus_tag="PL106_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNA33766.1"
                     /translation="MSRNGRILVAMSGGVDSSVAALLLHEQGHEVVGVTMKTWDYAEG
                     GAGKRITGCCDLDSINDARELAVTLGFQHIILDIREEFGSSIIGNFTDEYMAGRTPNP
                     CVLCNEKLKFGSLLAR"
BASE COUNT          411 a          498 c          539 g          232 t
ORIGIN      
        1 gtgaaggccg atacctccga attgcgacga agttccgtga cgagttcggc actgaaccgc
       61 gcgatgcccc cacggtacgg gggaaaggcg gagaggaagg cgaagcgcat ccggaacggc
      121 gaaggcctgc gatggctttc gttgcggaca aagatgcggg agcccggcag accttttgca
      181 caggatcatc cggtcgcgca agcgccacga ggatccctcg cacatcgcga acaacggcga
      241 atgcgcgtcc acagcacgaa tggccctctt cctcccgcgt tcacaaggta cctttgcctc
      301 caacctgcat gatcatccgc acctcattcg cgctggcctt cctcaccggt cctgtcctgg
      361 gcattatcgc acagccgcac tccgccatca accagaagga cgcgcagggc cgcaaacaag
      421 gcccatggca gcgcacatgg gcggacagtg accagatccg ctacgcgggc cagttcaagg
      481 atgacaagcc cgtgggcaac ttcagctact tcagcactgc gggcaaactc gagagcatcc
      541 tggcctggta ccccgatggc aaagccgcgc acgccaagca ctaccatccg gatggcaagt
      601 tgatggccga aggaagatat atcggtgaac agaaggacag cacctggaac tactacgacg
      661 tcaacggggc tctgcgctcc accgagcact ggaagaatgg caagtaccac ggcgagcagg
      721 aggcctactt cgccaacggc aaggtggccg agcgttgtgt atggaaggat ggtgaacgca
      781 acggcgagtg ccagcaattc ttcgagaatg gacaggtacg caccagcacg acctacgtga
      841 acggagaggc caacggtccc atgcgcgttc tcgccgagga cggcaagccg gagatcgagg
      901 gccagtacgc caaggacctg cgcagcggcc tctggaagca ctacaatacc gacggcagca
      961 tcctgatgca gacgctctac gaagcggaca aggtggtgaa ggagaagaag gagaacgggc
     1021 tgttccgcac cttctatccg gacgaccagc tgatgaagga ggagacgtac aaacacggca
     1081 agcgccacgg gaccttcacc gagtaccacg acaacggccg ctggacgatg cgccccacga
     1141 aggtgggacc ggacggtgcc gagaagaatg ataccgaacg cgtgctggag gggcagacca
     1201 aaaagcgcga gggccagtac aagaatgatc tgcaggaagg tgagtggaag gaatgggacg
     1261 aaaaaggcaa gctgatcggc accacggtgt acaaggccgg ggtcgcccaa tgacaaggac
     1321 aaccccatga gccggaacgg tcgcatcctg gtggccatga gcgggggcgt ggacagctcc
     1381 gtggccgcgc tgctgttgca cgagcaaggc cacgaggtgg tgggcgtcac catgaagacc
     1441 tgggactatg ccgagggcgg agcgggcaag cgcatcacgg gctgctgcga cctggacagc
     1501 atcaacgacg cgcgcgaact ggcggtgacc ctgggattcc agcacatcat cctggacatc
     1561 agggaggaat tcggcagcag catcatcggg aacttcaccg atgagtacat ggcgggccgc
     1621 acacccaacc cgtgcgtgct gtgcaacgag aagctgaagt tcggcagcct gctcgcccgc
//
LOCUS       DAOQOC010000773         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54338_length_1680_cov_3.257846, whole genome shotgun sequence.
ACCESSION   DAOQOC010000773 DAOQOC010000000
VERSION     DAOQOC010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54338_length_1680_cov_3.257846"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PL106_11650"
     CDS             <1..600
                     /locus_tag="PL106_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNA33767.1"
                     /translation="KDRRMRKAQAKEILYHEGDELRTLPLIVHGKVRTFKMNTDGKEL
                     VTGLYGPGDLLGYMGLLESGRATETAEMLEAGETALVPREDLLALLYRDRDVSVRFIK
                     LLARDVKEKEMRLLQFAYSTVRQRVAQALLHVHGRYATGDGGLGVSISRDDLAAIVGT
                     ATESLIRCLTGMKDEGLIDTHGRDIRIKDKVKLQKLAQG"
     gene            655..>1680
                     /gene="hemW"
                     /locus_tag="PL106_11655"
     CDS             655..>1680
                     /gene="hemW"
                     /locus_tag="PL106_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNA33768.1"
                     /translation="MPGIYLHIPFCRRACTYCDFHFSTSTRSRAAVLDAMRIEMDTRA
                     QEVSGAVGSIYFGGGTPSLLDPARIMDLMLQVRTLFLVNSDAEVTMEANPDDIDPDRL
                     DAWKAAGITRISLGTQSFRDDRLAWMGRAHTAQQALRGIDLIARSGFHSWTVDLIYGL
                     PDMSLQEWDEQLTIALDHGMPHLSAYCLTVEARTALAHQVKKRLVTMPDDTAQAGQFD
                     HLVARMEQAGLIPYEISNFGREGHIAVHNTNYWKGVPYIGIGPSAHSFDGSRRRWNVA
                     NNAVYLKAVPEGTPYFEEEMLTTAQRVNERLLTGLRTIWGVDLRTLGDDIRTRERATL
                     DKYMDQGD"
BASE COUNT          340 a          528 c          522 g          290 t
ORIGIN      
        1 aaagaccgcc gcatgcgcaa ggcccaggcc aaggagatcc tttaccacga aggcgacgag
       61 ttgcgtaccc tgccgttgat cgtgcatggc aaggtgcgca cgttcaaaat gaataccgac
      121 ggcaaggaac tggtcacagg gctgtacggc ccgggcgacc tgctgggcta catgggcctg
      181 ttggaaagcg gccgcgccac cgaaacggcc gaaatgctcg aggcgggtga aacagcgctc
      241 gtgccacgtg aggaccttct cgctttattg taccgggacc gcgatgtgag cgtgcgcttc
      301 atcaaactcc tcgcccgcga tgtgaaggag aaggagatgc gcctcctgca gttcgcgtac
      361 agcaccgtgc gccagcgcgt ggcccaggcc ctgctgcatg tgcatggccg gtatgccacc
      421 ggtgatggcg gcctcggggt cagcatcagt cgcgacgacc tcgcggccat tgtgggcaca
      481 gccacggaat cgctcatccg atgccttacc ggcatgaagg atgagggcct gatcgatacg
      541 cacgggcgcg acatccgcat caaggacaag gtgaagctcc agaagctggc gcagggctga
      601 gggcctttca cggtgtggcg gaacgggggt gtacggcgta cacttggtgc gcccatgccc
      661 ggcatttacc tccatatccc cttctgtcgg cgagcttgca cgtattgcga cttccatttc
      721 agtacttcaa cacggagccg cgcggccgtg ctcgatgcca tgcgcatcga aatggatacg
      781 cgcgcccagg aagtctccgg cgccgtgggc agcatctact tcgggggcgg tacacccagt
      841 ctgctcgatc cggcacgcat catggatctc atgctgcagg tccgtaccct gttcctggtg
      901 aacagcgacg cggaggtgac gatggaggcc aatccggacg atatcgatcc ggatcggctc
      961 gacgcgtgga aagccgccgg gatcacgcgc atcagcctcg gcacgcagag cttccgcgac
     1021 gaccggttgg cctggatggg tcgcgcacac accgctcaac aagccttgcg gggcatcgat
     1081 ctcatcgcac ggtccggttt ccattcctgg acggtcgacc tcatctacgg gcttccggac
     1141 atgtcgctcc aagaatggga cgagcaactc accatcgcat tggaccatgg catgccgcac
     1201 ctcagcgcgt actgcctcac cgtggaggca cggacagcgc tggcccacca ggtgaagaag
     1261 cgcctcgtga ccatgccgga tgatacggca caggccgggc agttcgatca cctcgtggca
     1321 cgcatggagc aagcgggcct cattccgtac gagatcagca atttcggtcg tgagggacat
     1381 atcgccgttc acaacaccaa ctactggaaa ggcgtgccgt atatcgggat cggccccagc
     1441 gcgcactcgt ttgatgggtc ccgacgccgt tggaacgtgg ccaacaacgc cgtgtatttg
     1501 aaagccgttc cggagggcac gccctatttc gaagaagaaa tgctcaccac cgcccaacgc
     1561 gtgaacgaac gcctgctcac cggcctgcgc accatctggg gcgtggacct gcggaccttg
     1621 ggcgacgaca tccgcacccg cgaacgtgcg accctggaca agtacatgga tcaaggcgac
//
LOCUS       DAOQOC010000774         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54353_length_1680_cov_2.753231, whole genome shotgun sequence.
ACCESSION   DAOQOC010000774 DAOQOC010000000
VERSION     DAOQOC010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54353_length_1680_cov_2.753231"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PL106_11660"
     CDS             <1..807
                     /locus_tag="PL106_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33769.1"
                     /translation="WPRGMPDCAGRDDRLNVLYCKRNATGNGVISRLRPKRDNVLKRS
                     RTMLENEHDLSYPCVIQHNGQILVLPGSSAGRLDLYRVDEANEALEFVRTILDEPLCA
                     PTLFQHHGRWWLFGTSPSKPDTHLHIFHSPDIEGPWLPHTGNPVKMDVRSARPAGTPF
                     IHQGVLYRPAADDSEGPGHSVALMRILELSPTSFREERVRTIGPIKGSAWSHGTGMLN
                     PVDGMTLVAGRRPMPPVRGSKGRKQRSSSSGKHRDRSPDRDQELEDEDDR"
     gene            804..>1680
                     /locus_tag="PL106_11665"
     CDS             804..>1680
                     /locus_tag="PL106_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33770.1"
                     /translation="MMLKHATGTVLARLFITGMNLLVMMLAGHALGAEGLGTIGLIVL
                     GITLVMLPANLIGGGAMVYLVPRVPLGRLLPPAYVCAMVSAAGSYLVLRKVPLVPDGF
                     EGHVCTLACIQAVYSAHFGVLLGRERIAAHNAINAIHSVVLLSAFALLLRFRNGSDAH
                     AYVYACYAAFTLSAVLSSAAMGRSVPVKLTNRHDVPRMLLRQGFWVQGANGLQLMNYR
                     LAYWIVEHTLGRAALGMYSVGTQLAESAWIAPRSLGMVLVSRVSNLEEVARQRDLTLL
                     TAKAAVGVALGVILVL"
BASE COUNT          309 a          531 c          540 g          300 t
ORIGIN      
        1 tggccgcgcg gtatgcccga ctgtgccggg agagacgacc ggttgaacgt gctctattgc
       61 aagcggaacg cgaccggcaa tggcgtcatc agccgattga ggcccaagcg cgacaatgtg
      121 ctcaagcgct cgcgcaccat gctggagaac gagcacgacc tgtcgtaccc gtgcgtgatc
      181 cagcacaacg ggcagatcct cgtactgccc ggaagttcgg ccggtcggct cgatctgtac
      241 cgcgttgacg aggcgaacga agcattggaa ttcgtccgga cgatattgga cgaaccgctg
      301 tgcgcaccta ccctgttcca gcaccatggc cgatggtggc tcttcggaac gtcgcccagc
      361 aagccggaca cccatttgca cattttccat tcaccggaca ttgaagggcc gtggctgcct
      421 cacaccggca atcccgtaaa aatggatgta cgcagcgccc gaccggccgg aaccccgttc
      481 atccatcaag gagtattgta cagaccggcc gctgacgaca gcgaagggcc cggccacagc
      541 gtggccctca tgcgcatcct ggaactcagc cccaccagct tccgagaaga acgggtacgc
      601 accattggcc cgatcaaagg ctcggcctgg tcccacggca ccggtatgtt gaaccccgtg
      661 gacggaatga cgctggtggc cggccgtcgg cccatgcctc cggttcgtgg cagcaagggc
      721 aggaagcaac ggtcaagctc cagcgggaaa caccgcgacc gatcaccgga tcgcgaccag
      781 gaactggaag acgaggacga tcgatgatgc tgaagcacgc caccggcacc gtgctcgcgc
      841 ggctgttcat caccggcatg aacctcttgg tgatgatgct ggcgggccat gcgctgggag
      901 ccgaaggact cggtaccatc gggctcatcg tactgggcat caccctcgtg atgctgccgg
      961 ccaacctgat cggtggtggc gcgatggtat acctggtccc ccgcgtgccc ttggggcgcc
     1021 tgcttccacc ggcatacgtg tgcgcaatgg tgagcgcggc gggatcgtat ctcgttttgc
     1081 gcaaggtccc cctggttccg gacggctttg aagggcacgt gtgcacgctc gcgtgcatcc
     1141 aggccgtgta cagtgcgcac ttcggcgtac tgctgggcag ggagcggatc gctgcgcaca
     1201 atgcgatcaa tgccatccac agcgtggtcc tgctgtccgc cttcgccctg ttgctcaggt
     1261 tccggaacgg gtcggacgcg catgcctatg tgtacgcctg ctacgccgcg ttcacgctca
     1321 gtgccgtgtt gagctccgcg gccatgggcc ggtcagttcc ggtgaagctc accaaccggc
     1381 acgatgtacc gcgcatgctg ctccgtcagg gtttctgggt gcaaggagcc aacgggttgc
     1441 aactcatgaa ctaccggctg gcatactgga tcgtggagca cacactgggc cgcgccgcct
     1501 tgggcatgta cagcgtgggc acccagttgg cggaaagcgc ctggatcgcc ccacgaagcc
     1561 tcggcatggt gctggtgagt cgcgtgagca atctggagga agtggcgcgt cagcgcgacc
     1621 tcaccctgct cacagcgaaa gcggcggtgg gcgtggcgct cggggtcatt ctggtgctcg
//
LOCUS       DAOQOC010000775         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54405_length_1679_cov_3.304187, whole genome shotgun sequence.
ACCESSION   DAOQOC010000775 DAOQOC010000000
VERSION     DAOQOC010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54405_length_1679_cov_3.304187"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1679)
                     /locus_tag="PL106_11670"
     CDS             complement(<1..>1679)
                     /locus_tag="PL106_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNA33771.1"
                     /translation="GTAPPIVPNLTITPASCSNGCDGTAGVAPTGGTGPFTYEWEPGA
                     PPGDGTAAVTGLCPGDWTVLITDTSIGCDTLVSFTIDAPLALDVQLITTDATCSGSCD
                     GTGSITINGGTPGYVIVWSPGAIIGQGTPSATQLCAGAYTVTVSDAAGCDTTIQFTIG
                     GPDPIGANAVITDITCNGACDGAIILAPTGGTPGYTYAWSPTGGNGPGISSLCAGTWT
                     ATITDAAGCDTTFSVGITEPQPLEAELIFTDAGCVPGCDGTASVVATGGTPDLVFDWS
                     GTPTGDGTPDVSGLCGGAWNVVVSDQNGCDTTIAFTIGMPPPIDPALVVTDATCANTC
                     DGSATVAPVGGPFAYDWEPGTINGDGSNAVADLCAGAYTVLITDPQSGCDTLVPFVVG
                     SAPALDVQLTATDLTCNGGCDGTATLAINGGTPGFVITWGPGIIIGQGTPNATQLCAG
                     AYTVTVSDAAGCDTTIQFTINGTVPIEPNITATNVTCNGYCNGTITASPSGGIPGYTY
                     VWSPPPTVGDSTASVSVLCAGIWTVTITDAAGCDTTVSTGITEPDPLEVTF"
BASE COUNT          283 a          541 c          550 g          305 t
ORIGIN      
        1 cgaaggtcac ctccaagggg tccggttcgg tgatccccgt ggataccgtg gtgtcgcagc
       61 cggccgcatc ggtgatcgtt accgtccaga tgcccgcgca gagcaccgag acgctggccg
      121 tgctgtcgcc cacggtgggc gggggcgacc atacatacgt gtatccgggt atgcctccgg
      181 atgggctggc cgtgatggtg ccattgcaat agccgttgca cgtgacgttc gtggccgtga
      241 tattcggctc gatcggtacc gtgccgttga tggtgaactg gatggtggta tcgcaacccg
      301 ccgcatcgga tacggtaacg gtatacgcgc ccgcgcacag ttgcgtagcg ttcggtgttc
      361 cctggccgat gatgatcccc ggtccccaag tgatcacgaa gcccggggtt ccaccgttga
      421 tggcgagcgt tgcggtacca tcgcatccgc cgttgcaggt gaggtcggta gcggtcaact
      481 gcacgtccag tgcgggggcc gatccgacga cgaacggcac cagggtatcg cagccgctct
      541 gggggtcggt gatgagtacg gtgtacgccc ccgcgcacag gtccgctacc gcgttcgagc
      601 catccccgtt gatcgttccc ggctcccaat cgtaggcgaa ggggccgcct accggcgcca
      661 cggtggccga tccgtcacag gtattcgcgc aggtggcatc cgttaccacg agtgcgggat
      721 cgatcggggg aggcatgccg atggtgaagg cgatcgtggt gtcgcaaccg ttctggtcgc
      781 tcacgaccac gttccaagcc cctccacaaa gcccggaaac gtccggtgta ccgtcccccg
      841 tcggcgtacc cgaccaatcg aagacaagat ccggtgtgcc tccggtggcc accacactcg
      901 cggtgccatc gcaacccggc acacaacccg catccgtgaa gatgagctcc gcctcgaggg
      961 gctgaggctc cgtgatcccg accgaaaatg tggtatcgca cccggccgca tcggtgatgg
     1021 tggccgtcca ggtccctgca cagagcgacg agatccccgg tccattccct ccggtcggcg
     1081 accaggcata ggtgtagccg ggggtccccc ccgtgggcgc caggatgatg gcgccatcgc
     1141 aggctccgtt gcatgtgata tcggtgatga cggcgttcgc tccgatcgga tccgggcccc
     1201 cgatggtgaa ctggatggtc gtgtcgcagc ccgccgcatc agatacggta acggtataag
     1261 cacccgcgca cagttgcgta gcgctcggtg tcccctggcc gatgatcgca ccaggtgacc
     1321 agacgatgac atagccgggt gtgccgccat tgatcgtgat cgagcccgtc ccatcgcacg
     1381 atccgctgca tgtcgcatcc gtggtgatca gttgcacatc aagcgccagc ggtgcgtcga
     1441 tggtgaatga taccaatgtg tcacagccga tcgaggtgtc cgtgatgagt accgtccagt
     1501 cgcccggaca gagccctgta acggccgccg tgccatcgcc cggcggcgcg cccggctccc
     1561 actcgtacgt gaacgggccc gtcccacccg taggagcaac gcctgccgtg ccatcgcatc
     1621 cgttcgagca tgaggccggt gtgatggtga ggttcggaac gatcggcggc gcggttccg
//
LOCUS       DAOQOC010000776         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54758_length_1674_cov_3.348981, whole genome shotgun sequence.
ACCESSION   DAOQOC010000776 DAOQOC010000000
VERSION     DAOQOC010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54758_length_1674_cov_3.348981"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PL106_11675"
     CDS             complement(<1..766)
                     /locus_tag="PL106_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA33772.1"
                     /translation="MSTGSSTSGKAFDARLFARLMTFVRPYRKLFWSCFALTILLSGI
                     TVVRPVLMGHMIDGPAAHGDRPGLVVLLSIVVGLLVLETVLGFVQAFWTARLGLNVTF
                     DLRQKLYEKILNFKLRWYDRTPVGTLVTRVVSDIETVEDIFSQGLLMIMGDILKLVVV
                     VVVMFAVNWELALLSMTPIPVLLWATNIFKNSIAKSFQDVRTQVARLNAFTQEHIQGM
                     AITQLFAREEQEQDRFTAINREHRAAHHSAQAAELRP"
     gene            complement(763..1527)
                     /gene="truA"
                     /locus_tag="PL106_11680"
     CDS             complement(763..1527)
                     /gene="truA"
                     /locus_tag="PL106_11680"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNA33773.1"
                     /translation="MEILPRYFLEIAFDGTPYRGWQIQPDAPSVQAEVQRALQLALHR
                     HQVIVVGCGRTDTGVHASSFYLHFDGPSDHTLNERFLQSLNAILPDSIAVKRIITVPD
                     DAHARFSATERGYVYKVHRWKDPFLHDRSHLLKPELDVASMNEACACLIGQQDFSSFQ
                     RTGSDNRTSICEVRHARWEPTDVGYQFTIRADRFLRNMVRAITGLSFRIGRGLHPAAY
                     MQEALEARDRAKAGRSAPACGLYLAHVSYPFIPSVA"
BASE COUNT          310 a          510 c          551 g          303 t
ORIGIN      
        1 ccggccgaag ttccgctgct tgggcggagt gatgcgccgc acggtgctcg cggttgatgg
       61 ccgtgaagcg atcctgctcc tgttcttccc gcgcgaacaa ctgcgtgatg gccatgcctt
      121 ggatgtgctc ctgggtgaag gcgttgagcc gtgccacctg cgtgcgcacg tcctggaagc
      181 tcttggctat gctgttcttg aagatgttgg tggcccacaa cagcacgggg atcggtgtca
      241 tgctcaacaa ggccagttcc cagttcacgg cgaacatcac gaccaccacc accaccagct
      301 tgaggatgtc gcccatgatc atgagcagcc cctgcgagaa gatgtcctcc accgtctcga
      361 tgtcgctgac cacccgtgtg accagcgtgc ccaccggggt gcggtcgtac cagcgcaact
      421 tgaagttgag gatcttttcg tagagcttct gccgcaggtc gaaggtgacg ttgaggccca
      481 gacgcgccgt ccagaacgcc tgcacgaagc ccagcaccgt ttcgagcacc agcagcccca
      541 caacgatgga cagcagtacg accaggccgg ggcgatcgcc gtgcgcggcc gggccgtcga
      601 tcatgtggcc cataagcacc ggccgcacca cggtgatgcc gctgagcagg atggtgagcg
      661 cgaagcacga ccagaaaagt ttccggtagg gacgcacgaa ggtcatcaac cgggcgaaga
      721 ggcgcgcgtc gaaggccttg cccgaagtgc tgctgcccgt actcatgcca ccgaagggat
      781 gaaggggtat gacacgtgcg ccaggtagag cccgcaggcc ggggcgctcc ggcccgcttt
      841 cgcccggtcg cgcgcttcga gcgcctcctg catgtaggcg gccgggtgca atccgcgccc
      901 gatgcggaag ctgagtcctg tgatggcccg gaccatgttc cgcaggaagc ggtccgcccg
      961 gatggtgaat tgatacccga catccgtggg ctcccaccgc gcatggcgca cctcgcagat
     1021 ggacgtgcgg ttgtcgctgc ccgtgcgctg gaagctggag aaatcctgct ggccgatgag
     1081 acacgcgcag gcctcgttca tggacgccac atccagctca ggcttcagca ggtgtgaacg
     1141 atcgtgcagg aacgggtctt tccagcgatg taccttgtac acatagccgc gctcggtggc
     1201 gctgaagcgg gcgtgggcat cgtcgggcac ggtgatgatg cgcttcaccg cgatgctgtc
     1261 cggcaggatc gcgttcaggc tttgcagaaa gcgctcgttc aaagtgtggt ccgatggccc
     1321 gtcgaagtgc aagtagaagc tgctggcatg cacgcccgtg tccgtacgac cgcagcccac
     1381 cacgatcacc tgatgccggt gcagggccag ttgcaaggcg cgctgcacct cggcctgcac
     1441 gctgggcgcg tcgggctgga tctgccagcc gcgatagggc gtgccgtcga aggcgatctc
     1501 gaggaagtag cgcgggagga tctccactcg ttcatgtgct catggccact ccgataattc
     1561 agaatgccca tggcggggcg cgaaagtaga ccggggggaa agcgcacagg ttgaacacct
     1621 tgacccgtcc taatctgggt ttacaggttt agggttgtgt ccgaagatag gttt
//
LOCUS       DAOQOC010000777         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54811_length_1673_cov_3.993820, whole genome shotgun sequence.
ACCESSION   DAOQOC010000777 DAOQOC010000000
VERSION     DAOQOC010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54811_length_1673_cov_3.993820"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PL106_11685"
     CDS             complement(<1..288)
                     /locus_tag="PL106_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33774.1"
                     /translation="MHRVALLSRSVWLLVIWAAGMLCSAQDRGSLTAEFARMSAKERA
                     RIAAKETDEAAKDAAFQAVMARAEQAFREGRYDDALHGYEEARVMRPYNVHP"
     gene            490..1356
                     /locus_tag="PL106_11690"
     CDS             490..1356
                     /locus_tag="PL106_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33775.1"
                     /translation="MKKTLFTALCGLTLLWSCQNDPTQSEQYKHLEHEHDSVSAQSGG
                     KDTTINNLLSAFNNVSENLRAIKEKQGVLGKPLDGQVETTPDMEKRIAADLYIIDSLL
                     SDNKKLIERMRRNASANKSKINELEKTLQTTIANMELTIAEKDSEIAGLKEQLTSTNA
                     SLASMITMYQDKEQLATAQRAELNKAYYCVGTGKELKDNGVVTKEGGFVGIGKSTKLN
                     TQGMNMEYFKQVDITQVLEIPVNAKKAKLITSHPANSYKMEGPDGRVDKLTIVDPNAF
                     WSVSKYLVVQAD"
     gene            complement(1424..>1673)
                     /locus_tag="PL106_11695"
     CDS             complement(1424..>1673)
                     /locus_tag="PL106_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33776.1"
                     /translation="KMAVVKQDRFNQAIASMKDHLEEAQYVKLETMRKNGELAGLCSA
                     DCSAKAGKSGCCAGKSGSAKSTTGAKSEDMHAPKPAVQ"
BASE COUNT          373 a          510 c          494 g          296 t
ORIGIN      
        1 gggatgcacg ttgtacggcc gcatgacccg ggcttcttcg tagccgtgga gcgcatcgtc
       61 gtatcgtcct tcgcggaacg cctgttcggc acgtgccatc accgcttgga aagccgcatc
      121 cttggccgcc tcgtcggtct ccttggcggc gatgcgcgcg cgttctttgg cgctcatcct
      181 ggcgaattcc gccgtgagac tgcccctgtc ctgggcgctg cacaacatcc cagcggccca
      241 tatcacgagc aaccagaccg accgggagag gagggcgacg cggtgcatcg ggcgaacgta
      301 catggaaccg gctggtgcgg ccccttctac gccaagtacc ggctcggcgt tcatctgccc
      361 gtgcggatcc cgttcgtcct cgtcgagcgg catcgaccct tcgacgaacc agccggggcg
      421 tccggataag gtccaatacc gccacctttg cacccggcgt tactttgaac gcatcaaacc
      481 cccaagttca tgaaaaagac attgttcacc gctttatgcg gcctcaccct gctctggtcg
      541 tgccagaacg accccaccca gagcgagcag tacaagcatc tggaacacga gcacgattcg
      601 gtcagcgccc agagcggcgg gaaggacacc acgatcaaca acctgctgtc ggccttcaac
      661 aatgtgagtg agaatctccg tgccatcaag gagaagcagg gcgtgttggg caaacctctc
      721 gatggccagg tggagaccac cccggacatg gaaaaacgga tcgctgcgga cctgtacatc
      781 atcgattcgt tgctgagcga caacaagaaa ctgatcgagc gcatgcgccg gaatgccagc
      841 gcgaacaaga gcaagatcaa cgagttggag aagaccctgc agaccacgat cgccaatatg
      901 gaactgacca tcgcggagaa agacagcgag atcgcggggt tgaaggagca gctcaccagc
      961 accaatgctt cgctggccag tatgatcacc atgtaccagg acaaggagca actggccacc
     1021 gcccagcgcg ccgaactgaa caaggcgtac tactgcgtgg gtacgggcaa ggagctcaag
     1081 gacaacggcg tggtgaccaa ggaaggcggt ttcgtaggga tcggcaagag cacgaagctg
     1141 aacacccagg gcatgaacat ggaatacttc aagcaggtgg acatcacgca ggtgctggag
     1201 atcccggtga acgccaagaa ggccaagctg atcaccagcc atccggccaa cagttacaag
     1261 atggaaggcc ccgatggccg cgtggacaaa ctgacgatcg ttgacccgaa cgccttctgg
     1321 agcgtgagca agtaccttgt ggtgcaagcc gactgaggtc gacatcacta aagaaaaagc
     1381 cccggcggat ccgccggggc ttttgtttgg tgcgttcgat gccttactgt acagcaggct
     1441 tcggggcatg catgtcctcg gacttggcac cggtggtgct tttggccgag ccggacttgc
     1501 cagcgcagca accggacttg cccgccttgg cactgcagtc ggccgaacag aggcctgcca
     1561 gttcaccgtt cttgcgcatg gtctcgagct tcacatactg cgcctcctcc aggtggtctt
     1621 tcatcgaggc gatcgcctga ttgaaacggt cctgcttcac cacggccatc ttg
//
LOCUS       DAOQOC010000778         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54816_length_1673_cov_3.586527, whole genome shotgun sequence.
ACCESSION   DAOQOC010000778 DAOQOC010000000
VERSION     DAOQOC010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54816_length_1673_cov_3.586527"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PL106_11700"
     CDS             <1..365
                     /locus_tag="PL106_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNA33777.1"
                     /translation="GTLAHFVKSLFGPEVTIRMRPSYFPFTEPSAEVDMSCTVCGGKG
                     CNLCKHSGWVEIMGCGMVDPAVLTNCGIDPGEYSGFAFGMGVERIAQLIYRVPDLRLY
                     WENDVRFLEQFSDGAFRA"
     gene            378..1652
                     /locus_tag="PL106_11705"
     CDS             378..1652
                     /locus_tag="PL106_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNA33778.1"
                     /translation="MTSGPLRTEVLIVGAGPGGCAAALSLARQNVPAILVDKASFPRD
                     KTCGDALSGKVMRALDRLAPGVGQGLRTDTRQLPSWGVTFTAPGGRSLRVPFSRETGM
                     EAAPGAIMPRLQFDALLVDEVRKAPGITLREGITLSTFERTPSGWLAMSADNGTRIEA
                     RLIIAADGANSRFAKHVAGLPMEPRHHCAGVRAYFHGVQGLDPLGFIELIFLRELLPG
                     YLWIFPLPGGRANVGLGLRSDVAARRKADLKALLRDLIATHPSLKYRFAHARMEGTIQ
                     GMGLPLASKRRALSGPGYLLVGDAGHLIDPFTGEGISHAMISGMHAGDVAAEALVRGT
                     GPPAAADLADYDGRVWRRLGKELAISARLQQLANMPWLFDLVVDRATRNPVLADTISS
                     MFNDLDLRARLRSPAFYARLLFGRSTPAARPD"
BASE COUNT          272 a          574 c          547 g          280 t
ORIGIN      
        1 aaggcaccct ggcccacttc gtgaaaagcc tgttcggccc ggaggtcacc atccgcatgc
       61 ggcccagtta cttccccttc accgagccga gcgccgaagt ggacatgagt tgcaccgtgt
      121 gcggcggcaa gggctgcaac ctgtgcaaac acagcggctg ggtggagatc atgggctgcg
      181 gcatggtgga ccccgccgtg ctcacgaact gcggcatcga ccccggggag tacagcggct
      241 tcgccttcgg catgggcgtg gagcgcatcg cgcagctcat ctaccgcgtg cccgacctgc
      301 ggctctactg ggaaaatgat gtccgtttcc tggaacaatt ctcggacggg gcgttccggg
      361 cctgacgggc acaggacatg accagcgggc cgctgcgcac cgaagtgctc atcgttggtg
      421 ccggccccgg tggctgcgcc gccgccctgt ccctggccag gcagaacgtt cccgcgatcc
      481 tggtggacaa ggcctccttt ccgcgcgaca agacctgcgg cgacgcgctc agcggcaaag
      541 tgatgcgcgc cttggaccgc ttggcacccg gtgtgggtca ggggctccgt accgacaccc
      601 gccagctacc gagctgggga gtcaccttca cggcccccgg cgggcggtcg ctgcgggtgc
      661 ccttcagtcg cgagaccggt atggaagccg ctccaggggc catcatgcca cgcctgcagt
      721 tcgatgcgtt gctggtggac gaagtgcgaa aagctcccgg catcaccctc cgtgaaggga
      781 tcacgctgag cacgtttgaa cgcaccccct ccggctggct cgccatgagc gcggacaacg
      841 ggacccggat cgaagcacgg ctgatcatcg cggccgatgg ggccaactcg cgcttcgcga
      901 agcacgtggc cggcctgcct atggagccgc gccaccactg tgccggcgta cgcgcctact
      961 tccacggtgt gcagggcctg gatccgttgg gtttcatcga actcatcttc ctgcgcgaat
     1021 tgctgcccgg ctatctctgg atcttcccgc tgcccggcgg ccgggccaat gtgggcctcg
     1081 gcctgcgcag tgatgtggcc gcgcgacgca aggccgacct gaaggcgctg ttgcgcgacc
     1141 tgatcgccac gcacccgtcg ttgaagtacc gcttcgccca tgcacgcatg gaagggacca
     1201 tccagggcat ggggctgccg ttggccagca agcgccgcgc tctcagcggc ccgggctatc
     1261 ttctcgtggg cgatgccggg cacctcatcg accccttcac cggcgaaggc atcagtcatg
     1321 cgatgatcag cggcatgcac gcgggcgatg tggccgccga agcgctcgta cgcggtacgg
     1381 ggccgcccgc cgcggcagac ctggccgact atgatgggcg ggtctggcga cgactcggca
     1441 aagaactggc catcagtgcg cgcctgcaac aactggccaa tatgccctgg ttgttcgatc
     1501 tggtggtcga tcgtgcgaca cgcaaccccg tactggcgga cacgatcagc agcatgttca
     1561 acgacctgga cctgcgcgcg cgcctgcgaa gcccggcatt ttacgccagg ctcctgttcg
     1621 ggcgcagcac acccgcggcc cggcccgatt aggccgtcct caacatccga tcg
//
LOCUS       DAOQOC010000779         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54953_length_1671_cov_3.763614, whole genome shotgun sequence.
ACCESSION   DAOQOC010000779 DAOQOC010000000
VERSION     DAOQOC010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54953_length_1671_cov_3.763614"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PL106_11710"
     CDS             complement(<1..739)
                     /locus_tag="PL106_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33779.1"
                     /translation="MAQLPATVDIMLANTGTPDSLEVRLRANGAPFSELVTEVTFTLA
                     WPSTSTATIGGRTVPCFDALPFAPSPMVTDGDWHYVTHHAIALQLLDEVCPSNTWPAD
                     TWVPVMRIKVDGLVGCVPFAIVNDAFTAANNRDFFVSLNGIEAPGVILSGPVDVGNCG
                     GLPDCLGVPGGPALPGTTCDDGDVCTSTDTWGADCVCAGTFVDTDGDGSCDAQDGCPA
                     DPLKVEPGICGCGTADTDTDADGTADCN"
     gene            complement(1236..>1671)
                     /locus_tag="PL106_11715"
     CDS             complement(1236..>1671)
                     /locus_tag="PL106_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33780.1"
                     /translation="LAFLGGTPQPGGTWTGPGGSPHSSTFIAGTDPYGLYTYTIAANG
                     PCAASQSVLAVLCETGIAEGAARGALRWLGADGAGLQTFDGPAMRNAAIDVLDAAGRS
                     VMHRTTMTLTTRWQLATDMLVPGSYTLVVRHAEGTAFVRFAR"
BASE COUNT          342 a          513 c          523 g          293 t
ORIGIN      
        1 cgttgcaatc ggccgtgcca tcggcatcgg tgtcggtgtc cgccgtaccg catccacaaa
       61 tgcccggttc aaccttcagg ggatccgcag ggcagccgtc ctgtgcatca caactgccat
      121 ccccgtcggt atccacaaat gtcccggcac acacacaatc agccccccaa gtatcggtag
      181 acgtgcagac atcaccatca tcacaggtcg ttccggggag ggccggaccg cccggcacgc
      241 cgaggcaatc ggggaggccg ccgcaattgc ccacatccac gggcccgctt aagatgaccc
      301 cgggcgcttc aataccattc agcgaaacga aaaagtcgcg attgttggca gcggtgaagg
      361 catcgttcac gatagcgaag ggtacgcagc cgaccaaccc atcgaccttg atccgcataa
      421 cgggaaccca tgtatcggcg ggccatgtgt tcgacggaca gacctcatcg agcagttgga
      481 gggcaatggc gtggtgggtg acgtagtgcc agtcgccatc ggtgaccatg ggagaaggcg
      541 cgaatggcag cgcatcaaaa caggggaccg ttcgcccccc tatggtggcg gtggaagtgg
      601 acggccatgc caacgtgaag gtgacctcag tgaccagttc gctgaagggc gcaccgttgg
      661 cacgcaacct tacttcaagg ctgtccggcg taccggtgtt ggcgagcatg atgtccaccg
      721 tagccgggag ttgggccatt gtgcacgtgg ccaacaaggg gaccgcgagc accaaggatc
      781 gcttggcatc gcgagggtat gaacgatcag ggcgcatggg atattgaacg cttgtacgcg
      841 ggaacagaga tagtcgttcg ctcgctcgtt gtacatgacg atccgacaca accaaccgcg
      901 gcagcgatat ggacacctgg agccctgctg aacgggcctc tcaggtgcag aaaaatggag
      961 cggggaacac cggaaggcgc tccccgctcc cattcggccg agccgtggcc cgatcggatg
     1021 tcctgagggc ttcgtttccc gttggggcgt tgctggcgca cgccttgtat gagccgctgt
     1081 tcctcttacc aggtgtcctc ccttgccttg cggcgagggg cgacgcgact cacgggttgg
     1141 gtctctgctc taaggtgagg cgaacataac cggaccctga aaaaaggcgt ccgttgaact
     1201 ttcaacaaat tgcgaacagg ccgatcacct ctgcttcacc gagcgaaccg gacgaatgcg
     1261 gtcccctcgg catgacgcac caccagtgtg tagctccccg gaacgagcat gtccgtggcg
     1321 agttgccagc gggtggtcag ggtcatcgtt gtacgatgca tgacggaacg ccctgcggca
     1381 tccagcacat cgatcgcggc gttccgcatg gccgggccat caaaggtctg cagaccggcc
     1441 ccgtcggcac ccagccaacg caatgcacct cttgccgcgc cctcggcgat ccccgtttcg
     1501 cagagtacgg ccagcacgga ttgggaagcc gcgcatggcc cgttggcggc gatcgtgtac
     1561 gtgtacaggc cgtacggatc agtgcccgca atgaatgtac ttgaatgcgg ggatccacca
     1621 gggcctgtcc acgtgcctcc gggttgtggg gtcccaccga ggaaggcaag c
//
LOCUS       DAOQOC010000780         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_54970_length_1671_cov_3.063738, whole genome shotgun sequence.
ACCESSION   DAOQOC010000780 DAOQOC010000000
VERSION     DAOQOC010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54970_length_1671_cov_3.063738"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1671
                     /locus_tag="PL106_11720"
     CDS             <1..>1671
                     /locus_tag="PL106_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33781.1"
                     /translation="STEWLACSYHDAPTSCSATGAVQGVWFTFTTTASTTDYTMYLLD
                     NSDNPAYTAPTLSATLYGGACSGVGADTEIQCIPDAGGTTLLTLATSTTYRLLVYNQG
                     GLAEGSFGLLITRPGVNDAGITAVAQPTAGVCDQKFQPEVTLTNLGQNAITSATITSY
                     IDATEVGTYAWSGTLAPGASEVVILPITSTPLGLHTYTASVTMVNGVPDEQLANSASS
                     SNYDASGQSVQVVISLDNDPGQTTWVLFDAFFFPVGSGGPYAGGDANTTVTEHICMPT
                     TYGNCFYFFLFDAAGDGISGGKWSLQDVNNKMILMDDGAYTNQSPSTTPGYSGYFAHE
                     FCLPLGPSPIQADECDVYNNYLNNKVYTTPVFGVMFYQFEFADPNAGWIRRISLPRNW
                     VKFGEMMSNPLVNGVTYYCRARADQGAAGFSDDHFGAGCELGLAPSQPVCTELISTPG
                     STFSCGATRVFGGSDKVWAQPVPYATQYRFRFTGNSFDPDGPGPMAPVNGIATRIVTQ
                     ASYALHLSWYTYALVAGNTYDVTVEAKVGGVWGGVCGGTCQVTISGNN"
BASE COUNT          323 a          539 c          496 g          313 t
ORIGIN      
        1 cttcaacaga gtggctggcc tgttcctatc acgatgcacc cacgagttgc tcggcgaccg
       61 gagcggtgca aggtgtgtgg ttcaccttca ccaccacggc gagcaccacc gactacacca
      121 tgtatctctt ggacaacagc gacaacccgg cgtacacggc tcccaccctc agcgcgacct
      181 tgtacggcgg ggcctgttca ggtgtcggtg cggataccga gatccagtgt attcccgacg
      241 ctggaggcac caccttgctc accttggcca ccagtaccac ataccgcctg ctggtgtaca
      301 accagggcgg cctggccgag ggctcctttg gtctgctgat cacacgaccg ggtgtcaacg
      361 atgcgggcat cacggcggtt gctcaaccga ccgcgggggt ctgcgatcag aagttccagc
      421 ctgaggtcac cttgaccaac ctcgggcaga acgcgatcac cagtgccacc atcacctcct
      481 acatcgatgc gaccgaagtc ggcacctacg catggagcgg tacgctggcg ccgggtgcga
      541 gtgaggtcgt gatccttccg atcacttcca caccgttggg actgcacacg tacacggcga
      601 gcgtgaccat ggtgaacggc gtaccggatg aacaattggc caacagcgca tcgagcagca
      661 attacgacgc cagcggccag agcgtgcagg tggtgatcag cttggacaat gatccgggtc
      721 aaacgacatg ggtcctgttc gacgcgttct tcttcccggt gggttccggc ggtccgtatg
      781 ccggtggtga tgccaacacc acggtgaccg agcacatctg catgcccacc acctacggca
      841 actgcttcta cttctttctg ttcgacgccg ccggtgacgg gatatcgggt ggaaagtggt
      901 cgttgcagga cgttaacaac aaaatgatcc tcatggacga tggggcgtac acgaaccaga
      961 gcccctcgac gaccccgggc tactcgggtt acttcgcgca cgagttctgc ctgcccctgg
     1021 gcccctcgcc catccaggcc gacgaatgcg acgtgtacaa caactacctg aacaacaagg
     1081 tgtacaccac cccggtgttc ggtgtgatgt tctaccagtt cgagttcgcc gatcccaacg
     1141 cgggatggat ccgccggatc agcctgcccc gtaactgggt gaagttcggt gagatgatga
     1201 gcaacccgct ggtgaatggc gtgacctact actgccgcgc ccgggccgat caaggagctg
     1261 ccggcttctc ggacgaccac ttcggtgctg gttgcgaatt gggcctggcc ccctcgcagc
     1321 ccgtatgcac cgaactgatc agcacgccgg gcagcacgtt ctcttgtggt gcgacgcgcg
     1381 tcttcggtgg cagcgacaag gtgtgggcgc agcccgtgcc gtacgccacc caataccgct
     1441 tccgcttcac gggcaacagc ttcgaccccg atggacctgg tccgatggcc cctgtgaacg
     1501 gcatcgccac ccgcatcgtc acccaggcga gctacgcgct gcacctgagc tggtatacct
     1561 atgcactggt agccggcaac acctacgacg ttacggtgga agccaaggtc ggcggtgtat
     1621 ggggcggtgt ttgcggtggc acctgccagg tgaccatctc gggcaacaac c
//
LOCUS       DAOQOC010000781         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_55168_length_1668_cov_3.474892, whole genome shotgun sequence.
ACCESSION   DAOQOC010000781 DAOQOC010000000
VERSION     DAOQOC010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55168_length_1668_cov_3.474892"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1127)
                     /locus_tag="PL106_11725"
     CDS             complement(189..1127)
                     /locus_tag="PL106_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA33782.1"
                     /translation="MGTLTERFLEHLAYERRSSALTVKAYGQDLSQFTSFLKDAVSLQ
                     HPEQANDKAIRAWMMSLMEQGTGPRSVQRKLSSLRAFYRFARMVGEVAADPTTLIDPP
                     KTPKRLPEFVAERPMEQLFRTVEWPEGFEGLRDRLMLELLYGTGMRLAELLGLTPADL
                     DPRRRTLRVLGKRNKERILPMAPPLVELLRNYMDARGRLTGAPGPHDPLLLDPDGEPV
                     PRRTVQRRVSHYLRAVTPQRKRSPHVLRHTFATHLLNNGADLNAVKELLGHASLAATQ
                     VYTHNTVEKLRKAHQQAHPRGGAIPSTNEHPDGSGL"
     gene            complement(1385..1576)
                     /gene="rpsU"
                     /locus_tag="PL106_11730"
     CDS             complement(1385..1576)
                     /gene="rpsU"
                     /locus_tag="PL106_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNA33783.1"
                     /translation="MLIIPVKEGEPIDKALKKFKKKFERTGTLRALRKRQSFTKPSVK
                     RRQQMLRAVYKLETYGGEQ"
BASE COUNT          310 a          489 c          518 g          351 t
ORIGIN      
        1 gcgcgagcgt caccgtctgt ccattgttca tgcgcaccgt cgtgctcaag ccgcaggaag
       61 acgtcgcccc cgatgatgtt atcgtggaat tgtgaaagtt tggcaagctt ctcgttgatg
      121 aaggtgacca gtttgctgtc agcgtcgaaa tggaccgatc tcacgttcac gttcatggtt
      181 tcccgttttt aaagccccga tccatcgggg tgttcgttgg ttgatgggat ggcgccacca
      241 cgcgggtggg cctgttgatg cgctttgcga agtttctcca cggtattgtg cgtgtacacc
      301 tgcgtggcgg ccaggctggc atgtcccagc aactccttca ccgcattgag gtcagcgcca
      361 ttgttcagca ggtgggtagc gaaggtgtgc cgaagcacgt gaggactgcg tttgcgttgc
      421 ggggtgaccg ccctaagata atgcgacacg cggcgttgta cggtacggcg cggcacgggt
      481 tcaccatccg ggtcaagaag cagcgggtcg tgggggcccg gggccccggt caggcgtcct
      541 cgcgcgtcca tgtagttgcg caggagttcg accagtggag gtgccatggg caggatgcgc
      601 tccttgtttc gtttgcccag cacccggagg gtccgtcgcc gaggatcgag atcagccggg
      661 gtaagaccaa gcaattcagc caagcgcatt ccggtgccgt acaacagttc cagcatcaaa
      721 cggtcgcgca ggccttcaaa gccttcgggc cattccaccg ttcggaacaa ctgctccatg
      781 ggtcgctccg caacaaattc gggcagtcgc ttcggggttt tgggcggatc gataagggtg
      841 gtgggatccg cagcaacctc acccaccatg cgggcaaagc ggtagaaagc ccgcagcgaa
      901 ctgagtttac gctgcacgct ccgcgggccg gtaccctgct ccatcaagct catcatccag
      961 gcccgaatgg ccttgtcgtt ggcttgctcc ggatgctgca ggcttacggc atctttcaaa
     1021 aaggacgtga actgcgagag gtcctgccca tacgccttga ccgtgagggc gctactgcgc
     1081 ctttcgtagg ccaggtgctc cagaaagcgt tcggtcaatg ttcccatgcg gcaccgatca
     1141 gttattgggc caacgaacgg acggcatcag cggatgttcg cggccctggc cacgtccccc
     1201 cgcccacacc cacgtaccgg atccaccttg cacagcaggc ttcataccca tgacaacgcc
     1261 ccccaaggcc ggatcatgtg gtgggggaac acaaaaaagg cgaaaagacc cgcaggtcct
     1321 ttcgcctatt gacgatcgtt gtggacaacg acgtgcgcac agcggggcgc cgggaacgtc
     1381 gcctctactg ttcgccgccg taggtctcca gcttatacac cgcgcgcagc atctgctggc
     1441 ggcgtttcac gctgggcttg gtgaacgact ggcgcttgcg cagggcgcgc agggtgccgg
     1501 tgcgctcgaa tttcttcttg aacttcttga gcgccttgtc gatgggctca ccttctttga
     1561 cagggatgat cagcatgcgg tcttgctaag ggggcgcgaa tataggcacg gaaagcatcg
     1621 gtgcaaatcc ggcgaggacg aacgtcaggt cttctgcttc ttcttgcg
//
LOCUS       DAOQOC010000782         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_55276_length_1666_cov_3.972688, whole genome shotgun sequence.
ACCESSION   DAOQOC010000782 DAOQOC010000000
VERSION     DAOQOC010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55276_length_1666_cov_3.972688"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..870
                     /locus_tag="PL106_11735"
     CDS             274..870
                     /locus_tag="PL106_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNA33784.1"
                     /translation="MIGTGELHELLAMHDPYVRPGKVEELAASFTRRTYRKGEVIISG
                     KGTCTTCVFLAEGFVHAFRTVRGEVQTMWFGEKGDLLTSYHTLFKNEPGDETVVALTD
                     CEVYSIDMVEFKRLVATDLEVAAIYIKVLEAGYSFWENRFLIRIQLAAEERYAEWLTR
                     TKHLAQHIPLGILARYLGIDQATLSRIRAKGGNKGSEG"
     gene            973..>1666
                     /locus_tag="PL106_11740"
     CDS             973..>1666
                     /locus_tag="PL106_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33785.1"
                     /translation="MYMRAPVVLAMILLSARTVSAQPPCTSGPPSASDNCPTATPIGL
                     NGVYCDNTCNFTANNASWFGSSGVFCGSGVNAQTIENNGFYRFQATASSVSLTFCVAP
                     GCTQRNSWIGASGIQVLVFNFPTPTGLGTCGSGSINGYYCLKQLSGMDCGSCGLPKGC
                     KTTTVSGLTSGKHYYIMIDGFEGDCCGFTIQATTNITLPVELISFTGEATRMGNDLRW
                     QTASERNNSHFDI"
BASE COUNT          345 a          490 c          495 g          336 t
ORIGIN      
        1 ccggtggtac ttcgtccatc cgttgctcaa ggcgaccagg gccggcttgc cgatggcctt
       61 tcgcagcttc accgggatcg agcgatgacc ggatgcccgt acgcgcccgc gagtgggaaa
      121 ggatgcgttc cccgatccgg ggataacttg gcgcgatgcc tctttccagc ggaccatgcg
      181 tttaagcgat ccgttcttga agggaccagg ggtcggttct accttttgac gctcacaagc
      241 catgatggaa caaatgaacc acgactggcc gcgatgatcg gaaccgggga attgcatgaa
      301 ctcctggcca tgcacgatcc atacgtgcgg ccgggcaagg tggaggaact cgccgcatcc
      361 ttcaccaggc gtacctatcg caaaggagag gtgatcattt ccggcaaggg gacatgcacc
      421 acgtgtgtgt tccttgccga gggttttgtc catgcctttc gtaccgtgcg gggcgaagtg
      481 cagaccatgt ggttcggcga gaagggcgat ctgctcacat cctaccacac cttgttcaag
      541 aacgaaccgg gggacgagac cgtggtggcc ctcaccgatt gcgaagtgta cagcatcgac
      601 atggtggagt tcaagcggct cgtagctacg gatctcgagg tggccgccat ctatatcaag
      661 gtgttggaag cgggctattc cttttgggag aaccgtttct tgatccggat ccaactggcg
      721 gcggaagaac gctacgccga atggttgaca cgcaccaaac acctggcgca acacattcca
      781 ctgggcatcc tggcgcgtta cctcgggatc gaccaggcca ccttgagcag gatacgggcc
      841 aagggaggga acaagggatc ggaagggtga tgggagcgca acaacgtcca tgtcgggccg
      901 tgtcgctttt tgacctgtgt caaaagatct gaatgggaac ggcgatattt ttccgcaact
      961 ccccattgga ccatgtacat gcgcgcgccg gtagtccttg cgatgatcct gctcagtgct
     1021 cgtacagtat cggctcaacc gccctgcaca tcgggtccgc cttcggccag tgacaattgc
     1081 cccacggcca cgccgatcgg cctgaacggt gtgtactgcg acaacacctg caacttcacg
     1141 gcgaacaacg cctcgtggtt cggatcgagc ggtgtcttct gcgggtccgg tgtgaatgcg
     1201 cagaccatcg agaacaacgg cttctaccgc tttcaggcca cggcctcttc ggtgagcctc
     1261 accttctgcg tagcgccggg ctgtacacaa cgcaacagtt ggatcggtgc gtcgggcata
     1321 caggtcttgg tcttcaattt tcccaccccc accggtctgg gcacctgcgg ttcgggcagc
     1381 atcaacggct attactgtct gaaacagttg agcggtatgg actgcggatc ctgcggcttg
     1441 cccaagggat gcaagaccac cacggtatcg gggctcacca gcgggaagca ctactacatc
     1501 atgatcgatg gcttcgaggg cgattgctgc ggattcacca tccaggcgac caccaacatc
     1561 acgctgccgg tggagttgat ctccttcacc ggagaagcca cccgcatggg caacgacctt
     1621 cgctggcaga ccgcatcgga gcggaacaac agccacttcg acatcg
//
LOCUS       DAOQOC010000783         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_55292_length_1666_cov_3.270639, whole genome shotgun sequence.
ACCESSION   DAOQOC010000783 DAOQOC010000000
VERSION     DAOQOC010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55292_length_1666_cov_3.270639"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..798
                     /locus_tag="PL106_11745"
     CDS             304..798
                     /locus_tag="PL106_11745"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNA33786.1"
                     /translation="MTQGQTIAAELTGLQAATRTLLERTPTDKLTWKPHEKSMTLGRL
                     AMHVAELPHWIVKSFELPEFDFIAAGFTPNVPTDHAQIMAMMADIWPKALAYLDKATD
                     ADLAQVWTMRRGDHVIWSMPRADLARLHIKHMVHHRGQLSVYLRLLDVPIPELFGPTA
                     DTRK"
     gene            801..1325
                     /locus_tag="PL106_11750"
     CDS             801..1325
                     /locus_tag="PL106_11750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNA33787.1"
                     /translation="MVRTVLAINGSTRASSSNGHYLHAIERLVGDRAAFVRSPSISDL
                     PHFSPDLDHEDPPASVVHFRQLVLASDAVLICTPEYAMGVVGSLKNALDWLVSSSSLS
                     GKPVMLVTASLSGEKGHAALLETLTVIEARLRPETNVLIPFAKTKVTSDGVITDDPAL
                     QAIQNGLDVLLASR"
     gene            complement(1347..>1666)
                     /locus_tag="PL106_11755"
     CDS             complement(1347..>1666)
                     /locus_tag="PL106_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin reductase"
                     /protein_id="HNA33788.1"
                     /translation="IAILRQMRVDGTLMGNSLFFFNKTRADLFMEEEFRSMLGDAFHP
                     MLSNEEAPGYLHGRVGAAFLSAHIKDFNQPFYTCGPEGFVKAVKSSLLELGAGESMID
                     VQF"
BASE COUNT          338 a          528 c          483 g          317 t
ORIGIN      
        1 ctgtatacgt ccggatcgtc gcgaacatgg agcatggcgc catacggcaa agggatctac
       61 ttcctctgtg ccgagttgaa cggtgtgagg cgcgtattcc gcttcgtggt gaaccgctga
      121 ccattcgcgg caacttcggt gatacagctt cgtgccgagc aggtgcatgc tcgatccgct
      181 gcgcaacatt ggtccggacg tgtgaagtgt catgcaagta catcccaagg gctcgacggc
      241 tgaccggcct gtcggaacgt tgcacagaac ccgcaatttc gcaccccgac caatactgga
      301 agaatgaccc aaggacaaac catcgcagca gagctcacag gactacaggc cgcaacccgc
      361 accctcctcg aacgcacccc caccgacaag ctcacatgga agccgcatga gaagtcgatg
      421 acactgggtc ggttggccat gcacgtggcc gaactgccgc attggatcgt gaaatcgttc
      481 gagctgcccg agtttgattt catcgcggct ggcttcaccc cgaacgtgcc gaccgatcac
      541 gcgcagatca tggcgatgat ggccgacatc tggcccaaag ccctcgccta tctggacaag
      601 gccaccgatg ccgatctcgc ccaggtgtgg accatgcgcc gtggtgacca tgtgatctgg
      661 agcatgcccc gtgcggatct ggcccggctc cacatcaagc acatggtgca tcaccgtggc
      721 cagttgagcg tgtacctgcg cctgctcgat gtgccgatcc ccgagctctt cgggcccacg
      781 gccgatacgc ggaagtgacc atggtccgga ccgtcctggc gatcaacgga agcacccgcg
      841 cctcgtcatc gaacggccat tacctccatg ccatcgagcg cctggtcggt gaccgcgcgg
      901 cattcgtgcg ttctccgtcc atctccgacc ttccgcactt cagcccggac ctggaccacg
      961 aagatcctcc ggcctcggta gtgcatttcc gtcaactcgt cctggccagc gatgccgtcc
     1021 tgatctgcac cccggaatat gccatgggtg tggtgggcag tttgaagaac gcgctcgact
     1081 ggttggtttc gtccagcagc ttgagcggca aacccgtcat gctcgtgacc gcctcattga
     1141 gcggcgagaa aggacatgcg gcgctgttgg agaccttgac cgtgatcgag gcccggctac
     1201 gaccggagac caacgtgttg atcccctttg cgaagaccaa ggtgacctcg gatggcgtga
     1261 tcaccgatga tccggcattg caggcgatcc aaaacgggtt ggacgtactg ctggccagtc
     1321 gatgaacgac ctgccacgca gcgccttcag aactgcacgt cgatcatgga ctccccggcg
     1381 ccgagttcca gcaatgagga cttcaccgcc ttcacgaaac cttcggggcc acaggtgtag
     1441 aacggctggt tgaaatcctt gatgtgtgcg ctcaggaaag cggcacccac acgcccgtgc
     1501 aggtagccgg gggcctcttc gttgctgagc atcggatgga acgcatcgcc cagcatggag
     1561 cggaactcct cctccatgaa caggtccgcc ctggtcttgt tgaagaagaa gagcgaattg
     1621 cccatcaatg tgccgtccac ccgcatttgg cgcagaatgg cgatga
//
LOCUS       DAOQOC010000784         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_55617_length_1662_cov_3.029247, whole genome shotgun sequence.
ACCESSION   DAOQOC010000784 DAOQOC010000000
VERSION     DAOQOC010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55617_length_1662_cov_3.029247"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1662)
                     /locus_tag="PL106_11760"
     CDS             complement(<1..>1662)
                     /locus_tag="PL106_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33789.1"
                     /translation="LTARAHDARNEENELIDRIGRIKLEESAARGGKKDELVRKRLEL
                     EAQLEALHQENERAVAKAGTAEQESASARGQAALARKLETGNTVADPFPEAERSRLAQ
                     QIASTGPRIGAIALDERFDGTSGIAASELERRTFDWGVPVTLDVDGGGGDILALDGGI
                     NTTGGDQGTITADKTTSGSKDTNTSNTPTGTNTGQASTGEQPNGQITAAAQQASGDQG
                     TTTADKAASGSTGTNASDTSAGTTTGQASTGEQPNGQATAQGADGRQGTATRDASTTG
                     NPEVASASVRAAGDTAMAPEARAGNNNIRVSDAGGRAAVDGAAGTSGVSNGRDPLVQG
                     SPSTDSGAPQATREGSHAISRAPDDVATTRGAPAGGVPDASPNGTATGAGNGREEGAL
                     IDGTASAPNTPTPEEIAFVQANELAELKQMRGAERDRQRRDSLDTRISELEQAMRAGS
                     RTDEAGELVGQQSGQQGSTGTGTGSTSGAEPTAPVSSDISSGAPERAAIAQRPALIFD
                     PGMSEGELVARSRVCGHVTMVSGRCPDGVGEIAVSEADVDNFGLV"
BASE COUNT          236 a          571 c          509 g          346 t
ORIGIN      
        1 tcacgagacc gaagttgtcg acgtcggcct cgctgaccgc gatctcgccc accccgtcgg
       61 gacagcggcc cgacaccatg gtcacgtggc cgcacacccg gctgcgcgcc accagctcgc
      121 cctcgctcat tcccggatcg aaaatgaggg cgggacgctg tgcgatcgcg gcacgctctg
      181 gtgcgccgct cgagatgtcc gaggaaacag gtgcggtggg ctccgcaccg ctcgttgaac
      241 ccgtaccggt cccggtggat ccctgctggc cgctttgctg cccgacaagc tcacctgcct
      301 catcagttcg ggagccggca cgcatcgcct gctccaattc cgagatgcgg gtatccagac
      361 tgtcgcggcg ttggcgatcc cgttcggcac cgcgcatctg cttcagttcg gccagttcgt
      421 tggcctgcac gaacgcgatc tcctctggcg tgggcgtgtt gggggccgaa gcggtcccat
      481 cgatcaacgc gccctcctcc cggccattcc ccgccccggt cgcagtgcca ttgggtgaag
      541 catcggggac accaccagca ggtgctccgc gcgtggtggc cacatcatcc ggtgctcggg
      601 aaatggcatg ggatccctca cgcgtagcct gcggggcccc ggaatccgtc gagggtgaac
      661 cctgcaccag tgggtcacgg ccattcgaca cgcccgaagt accggccgcg ccatcaacag
      721 cggcacgccc accggcatca gataccctga tattgttatt acctgcccgc gcttccggcg
      781 ccatggccgt atcacccgcg gcccgcaccg aagccgaggc tacttccggg ttgccggtag
      841 ttgacgcatc gcgggtcgct gtgccctggc gcccatccgc tccttgtgcc gttgcttgcc
      901 cgttcggttg ttcaccagtg gatgcctggc cggtggttgt acctgcggat gtatcggacg
      961 cgttggtgcc cgttgaacca ctcgccgcct tgtccgcagt ggtggtgccc tgatcaccgc
     1021 ttgcctgttg cgccgcagcc gtgatctgcc cattcggttg ctcaccagtg gatgcttggc
     1081 cggtgtttgt acctgtcggt gtgttggacg tgttggtgtc ctttgaacca ctggtcgtct
     1141 tgtccgcagt gatggtgccc tgatcaccgc ctgtcgtgtt gatgccacca tcaagagcaa
     1201 ggatgtcccc accaccaccg tcaacatcca aggtgaccgg caccccccaa tcgaaggtgc
     1261 gccgctcgag ttcgctagct gcgatacccg aagtaccatc gaagcgctcg tccaacgcga
     1321 tcgcaccgat gcgtggtccc gtgctggcga tctgttgtgc gagccgggac cgttcggcct
     1381 cagggaaggg atcagctacc gtgttcccgg tttcgagttt cctggccagg gcggcctgac
     1441 ctcgggcact cgccgattcc tgctccgccg tgcccgcttt ggccaccgca cgctcattct
     1501 cctggtgcaa cgcttcgagc tgcgcttcca gttcaagccg tttgcggacg agttcgtcct
     1561 tcttgccgcc ccgtgctgcg gactcctcga gtttgatgcg tccgatgcga tcgatgagtt
     1621 cgttctcctc gttacgggca tcgtgggcac gagcggtgag gg
//
LOCUS       DAOQOC010000785         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_55812_length_1659_cov_3.332294, whole genome shotgun sequence.
ACCESSION   DAOQOC010000785 DAOQOC010000000
VERSION     DAOQOC010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55812_length_1659_cov_3.332294"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PL106_11765"
     CDS             complement(<1..1636)
                     /locus_tag="PL106_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33790.1"
                     /translation="MFCLTDGPQAPDQVSGIFSTDDPANLIVDPNSGVIDVGASQPGT
                     YQVTQTVTSGACITIHTVTVELIAPPAVSTGSYGPTCTNGTVVALGGSPAGGAWSGNG
                     VIGGTFDPAIAGVGPHTVTYTVSNAACTASATTTIAVNEAPSASISYPGSPFCSNDGN
                     QGITFTGTPGGSFSSTAGLSLNSSTGEVDPAASGAGTWTVTYAIPATGGCAAFSTSCS
                     ITINAAPGASFSYNGPFCNNTGTQMPTFTGTTGGIFSAPAGLVLNSTTGQVDPGATTS
                     SASGTFYTVTYSIAAQGGCASFSTTANVMITQSPWAGTDGSLTICSNAAPATLFPLLG
                     GTPAGSGAWTNPVNAPHSGTYDPSVDGSGNYTYTVTGGPGCADDLSTALVTETPATSN
                     TTAVTACDSYLWPETGLTYTANGTYTDLSGCHTEVLQLTLNFPGTACDDGDAETGNDT
                     YDGTCNCIGLLIDCAGVPGGTATLDNCGQCVGGNTGNTACVQDCNGDWGGTAIIDNCG
                     QCVGGNTGNTACAQDCNGDWGGTATIDNCGNCVGGNT"
BASE COUNT          287 a          491 c          549 g          332 t
ORIGIN      
        1 cggtgttgcc gcccacacag ttcccgcagt tgtcgatcgt tgcggtgcca ccccagtcgc
       61 cgttgcagtc ctgagcgcaa gccgtattgc ccgtgttgcc acctacgcac tgaccgcagt
      121 tgtcgatgat cgccgtgccg ccccaatcgc cgttgcagtc ctggacgcaa gccgtgttgc
      181 ccgtgttgcc gcccacgcac tgaccacagt tgtccagggt cgcggtgccg ccgggaacgc
      241 cggcgcagtc gatcaagagg ccgatgcaat tacaggtgcc atcgtaggta tcatttccgg
      301 tttcggcgtc gccatcatcg caggcggtgc ccgggaaatt cagggtgagt tgaagcactt
      361 cggtatggca tccactcagg tcggtgtagg tgccgttggc ggtgtaggtc agccccgtct
      421 cgggccatag gtagctgtca caagccgtga ccgcggtcgt gttggatgtt gcgggtgttt
      481 ccgtcacaag ggcagtggag aggtcgtcag cgcagcccgg tcctccggtc acggtatagg
      541 tatagttgcc ggatccgtcc accgaggggt cgtacgtgcc tgaatgcggg gcattgaccg
      601 ggttcgtcca ggcgccacta ccggctggag taccgccaag gagggggaac aatgtcgcag
      661 gggccgcatt gctgcatatc gtgagtgatc cgtcagtccc cgcccacggc gactgggtga
      721 tcatcacatt ggccgttgtg ctgaacgacg cacaaccgcc ttgcgccgcg atactgtacg
      781 tcacggtgta aaaggtgccc gacgcggatg atgtggtcgc tccaggatcc acctggccgg
      841 tcgtgctgtt caataccaat ccagcgggtg cgctgaagat cccaccggtg gttccggtga
      901 aagtgggcat ctgtgtgccg gtgttgttgc agaacggccc gttgtaactg aacgaagcgc
      961 ccggtgcggc gttgatggtg atggaacagg acgtgctgaa cgcagcacaa ccacccgtgg
     1021 caggtatcgc gtacgtcacc gtccaggtgc ctgcaccgct ggcagctggg tccacttccc
     1081 cggtgctgct gttcaagctg aggccagcgg tcgaactgaa ggagcctcct ggcgttccgg
     1141 tgaaggtgat gccctggttg ccgtcgttac tgcagaatgg ggatcctgga tagctgatcg
     1201 aagcacttgg agcctcgttc acggcaatgg tggtggtggc actggccgtg caagcggcat
     1261 tgctcacggt gtaggtgacc gtatgcgggc cgacacctgc gatcgcgggg tcgaatgttc
     1321 caccgatcac cccgttgcca ctccacgcac caccggcagg cgagcctccg agggcgacaa
     1381 cggtgccgtt cgtgcaggtg ggaccatagc tgccggtgct cacggcaggg ggggcgatga
     1441 gttccacggt cacggtatgg atcgtgatac atgcgcccga agtgaccgtc tgcgtcacct
     1501 gataggtccc gggctgtgaa gcgcctacgt cgatcacccc gctgttcggg tccacgatca
     1561 ggttcgcggg atcatccgtg gagaagatcc cggaaacctg gtcaggcgct tgggggccat
     1621 ccgtgaggca gaacacccct gatgcataac tgaactccg
//
LOCUS       DAOQOC010000786         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56297_length_1652_cov_3.138384, whole genome shotgun sequence.
ACCESSION   DAOQOC010000786 DAOQOC010000000
VERSION     DAOQOC010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56297_length_1652_cov_3.138384"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PL106_11770"
     CDS             <1..415
                     /locus_tag="PL106_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HNA33791.1"
                     /translation="VHEFAHYSTFQKFRRHQPHGPEWKAEYRRLMRPFLSSAVFPADV
                     LHALEHHLADAPSSSCTDHGLMRTLRRYDPDPRPFLEELPASTVFRFQQKIFVKGERL
                     RKRYRCKCLNDRRIYHIDPTVEVQLEQTTPVRRAS"
     gene            466..1551
                     /locus_tag="PL106_11775"
     CDS             466..1551
                     /locus_tag="PL106_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNA33792.1"
                     /translation="MDPKHTYCVIMAGGIGSRFWPMSRTACPKQFLDFLGLGRTLIQQ
                     TWDRFLPICPPENILVVTNERYASMVKEQLPDMADANILTEPLRKNTAPCVAYANHVI
                     SKRDANASIIVASSDHLVLKDAEFQGVIRLALQQAGTMDCIVTLGIRPNRPDTGYGYI
                     QYGGEAGIPGVQRVISFREKPDHATAEKFVASGDFLWNSGIFIATAKGLHHAFAEHLP
                     AMELRFAQGAASYGTPAERAFIAEAYSTCESISIDYGVMEKAKNVFVVPGDFGWSDLG
                     TWGSLYTHVPKDAHGNAAIGALVRLHDCTNTMVHVQDDRLVVLQGLDDHIVVSTKDAL
                     LVVRKHDEQKIKQFVEQAGQEFGEKYV"
BASE COUNT          340 a          538 c          491 g          283 t
ORIGIN      
        1 tgtgcatgag ttcgcacatt acagcacctt ccagaaattc cgtcgccacc agccgcacgg
       61 ccccgaatgg aaggctgagt accgtcgcct catgcgcccc ttcctgagca gcgcggtctt
      121 cccggccgac gtgctccacg cgctggaaca ccacctcgcc gacgcgccca gcagcagttg
      181 caccgaccat gggctcatgc gcacactgcg ccgttacgac ccggatcccc ggcccttcct
      241 ggaggaactg cccgcatcca ccgtcttccg cttccagcaa aagatcttcg tgaagggcga
      301 gcgcctgcgg aaacgctacc ggtgcaaatg tttgaacgac aggcggatct accacataga
      361 ccccaccgtg gaggtgcagt tggaacagac caccccggtg cggcgggcgt cctgagtcgg
      421 cggcccgtgg ccgtatcgta aaaaagcgac cttcgcgccc cgatcatgga ccccaagcat
      481 acttactgcg tgatcatggc cggcggcatc ggcagccgct tctggcccat gagccgcacg
      541 gcctgcccca agcagttcct cgattttctc ggcctcggtc gtaccctgat ccaacagacc
      601 tgggatcgtt ttcttccgat ctgcccgccg gagaacatcc tcgtggtcac caacgaacgg
      661 tacgcgtcca tggtgaagga gcagctgccg gacatggccg atgccaacat cctgaccgaa
      721 ccgctgcgca agaacaccgc gccgtgcgtg gcctacgcca accacgtgat cagcaagcgc
      781 gatgcgaacg ccagcatcat cgtggcgtca agcgaccacc tggtgctgaa ggatgctgag
      841 ttccaaggcg tgatccgcct cgcgctgcag caagccggaa cgatggattg catcgtcacc
      901 ctcggcatcc ggcccaaccg gcccgatacg ggttatggat acatccagta cggcggcgaa
      961 gcgggtatcc cgggtgtgca acgcgtgatc agcttccgcg aaaaaccgga ccatgccaca
     1021 gcggagaagt tcgtggccag tggcgatttc ctctggaaca gcgggatctt catcgcaaca
     1081 gccaagggac tgcaccacgc cttcgcagag cacctgcccg ccatggaact gcgcttcgcc
     1141 caaggtgcgg ccagctacgg tacacccgcc gaacgtgctt tcatcgccga agcctactcc
     1201 acctgtgaaa gcatcagcat cgactacggt gtgatggaga aggccaagaa tgtgttcgtg
     1261 gtgcccggcg atttcggctg gagcgacctg ggcacatggg gcagcttgta cacgcacgta
     1321 cccaaggacg cccatggcaa cgcggctatt ggagcgctgg tgcgattgca tgattgtacc
     1381 aacaccatgg tacatgtgca ggacgaccgg cttgtggtgc tgcagggcct cgacgaccat
     1441 atcgtggtga gcaccaagga cgccctgctc gtggtgcgca agcacgatga acagaagatc
     1501 aagcaattcg tggagcaggc cggccaggag ttcggggaga agtacgtgta aagtgcacgg
     1561 atccgggcat cctgaccatg tgtgagcctg gcgcgggacc ctgcgccgcc gggcaattgc
     1621 cgctggtgtg tgacaggatc catgaccggc cc
//
LOCUS       DAOQOC010000787         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56505_length_1649_cov_3.483061, whole genome shotgun sequence.
ACCESSION   DAOQOC010000787 DAOQOC010000000
VERSION     DAOQOC010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56505_length_1649_cov_3.483061"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PL106_11780"
     CDS             <1..523
                     /locus_tag="PL106_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA33793.1"
                     /translation="LTPGVIGMGTAFKGMGLPTGTFTRADFLTHRPDRLFDVVCSFGF
                     IEHFTNWEEVLLKHAALVAPNGLIVLETPNFKGPVQGIFHRWLDAENLARHNQDAMDP
                     PRWAELLRMEGFEILEVGWFGRFTFWSDSKPGGTSQRIGRILLRWLTPLFERMGQRSR
                     TFSPYCGLVARKR"
     gene            520..1398
                     /locus_tag="PL106_11785"
     CDS             520..1398
                     /locus_tag="PL106_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HNA33794.1"
                     /translation="MTMAQGPPVITRLMGGLGNQMFQYATGLALSRRLGAPLLIDRTF
                     LDARPAGMNWTARELELDVFDLRIEAADPSLIRDLRRQRRPVAIRRQTWFRERDKRYD
                     PQFARLRAPVLLDGYWQSELYFGNIASELREQVFHQAGEPSQENLELLHLGASMSTAS
                     VHVRCGDYLMDPAAAAYHGRPTRAYYEAAAAELFEKHGVRHFFIFSDEPAKARTFVHL
                     PGGMTFVTHNTGRAAHWDLWLMRQCRYHIIANSSFSWWGAWLNPSPTKVVIAPNTWFA
                     GDPRPHDIVPSTWLRR"
     gene            1395..>1649
                     /locus_tag="PL106_11790"
     CDS             1395..>1649
                     /locus_tag="PL106_11790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA33795.1"
                     /translation="MRIAHCVESYLPAIGGMVEVVRQLSERMVRGGHEVTVLTSSHPD
                     RHDHTINGVRIREFAVSGNSVRGVRGEGSSYVDALLTGDFD"
BASE COUNT          301 a          535 c          497 g          316 t
ORIGIN      
        1 cctcactccc ggtgtcatcg gcatgggcac cgcattcaaa gggatgggct tgcccacggg
       61 cacattcacg cgcgcggatt tcctgacgca ccggccggac cggctcttcg atgtggtctg
      121 ttcgttcggc ttcatcgagc acttcaccaa ctgggaggag gtgctgctca aacacgccgc
      181 actggtcgcc ccgaacgggc ttatcgtact ggagaccccc aacttcaaag ggccggtcca
      241 gggcatcttc catcggtggc tcgatgcgga gaacctggca cgccacaacc aggacgcgat
      301 ggatccaccg cgatgggccg aactgctccg gatggaagga ttcgagatcc tggaggtcgg
      361 ctggttcggt cgtttcacct tctggagcga ctcgaagccc ggtggcacca gccagcgcat
      421 cggtcgcata ctgctccgtt ggctcacacc cttgttcgag cggatgggac aacgatcgcg
      481 caccttctcc ccctattgcg gcctggtggc ccgcaagcga tgaccatggc ccaaggaccg
      541 ccggtaatca cccgcttgat gggcggcctg ggcaaccaga tgttccagta cgccacgggg
      601 cttgcgctct cccgaagact cggtgctccg ctcctcatcg atcgcacctt tctcgatgcc
      661 aggcccgccg gtatgaactg gaccgcacga gaactcgagc ttgacgtgtt cgacctccgg
      721 atcgaagcgg ccgacccgtc actcatccgg gatctgaggc ggcaacgccg acccgtggcc
      781 atacgccgac agacctggtt ccgcgagcgc gacaaacgct acgacccgca attcgcgcga
      841 cttcgggctc ctgtactgtt ggacgggtac tggcagtccg aactgtattt cggtaacatc
      901 gcgagcgaac tgcgcgaaca ggtgttccac caagccgggg aaccttcgca ggagaacctg
      961 gaactcctcc acctgggcgc ctccatgtca acagccagtg ttcacgtgcg ttgcggagac
     1021 tacctgatgg accctgccgc cgcggcttat cacggccggc ccaccagggc gtactatgaa
     1081 gcggcagcag cggagttgtt cgagaagcat ggcgttcgcc actttttcat tttctcggat
     1141 gagccggcga aggcacgaac cttcgtccat ctgcccggcg ggatgacctt tgtgacccac
     1201 aataccggtc gtgccgccca ttgggacctc tggctcatgc gccaatgccg gtatcatatc
     1261 atcgccaaca gcagtttcag ctggtggggg gcctggctga acccgtcgcc gaccaaagtg
     1321 gtcatcgccc cgaatacctg gttcgcgggt gatcctcgtc cacacgacat cgttccatca
     1381 acctggctgc gccgatgagg atcgcccatt gcgttgagag ctacctgccc gcgatcggcg
     1441 gcatggtgga ggtggtacgt caactcagcg agcgcatggt gcgcggcggg cacgaagtga
     1501 cggtcctgac ctcctcgcat ccggacaggc atgatcacac catcaacggg gtacgtatcc
     1561 gcgagttcgc ggtcagcggc aattccgtgc ggggagtacg tggcgagggg tcgtcctacg
     1621 ttgacgcttt gctcacgggc gacttcgat
//
LOCUS       DAOQOC010000788         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56511_length_1649_cov_3.174404, whole genome shotgun sequence.
ACCESSION   DAOQOC010000788 DAOQOC010000000
VERSION     DAOQOC010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56511_length_1649_cov_3.174404"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PL106_11795"
     CDS             <1..357
                     /locus_tag="PL106_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33796.1"
                     /translation="VNEWDAGLATALVLARVRSAVTSSHRVSLWASILRGSLIFTKQY
                     LAGEITAAGMVAMASLVFMVVVLWSFLPGLRPWINPSPVLFVAVRRVVPRGSWETWDV
                     RAARAITARPLMTASR"
     gene            394..1533
                     /locus_tag="PL106_11800"
     CDS             394..1533
                     /locus_tag="PL106_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33797.1"
                     /translation="MFPRAAFLCPVILYLTYNDQPSGVYWSQVTDVVEHMNSLGGPEV
                     RLVALVSGRNFLRTRQAIKAHSPSSLVLPMVPAMRRWRMNTTLLAWVCRVLRPSGIIC
                     RGPFATWMALRMRDRGLTERVCFDGRGAYAAEWEEYRIIDNDALIAQVRPLEQEAVGA
                     SDFRLSVSNALVAHWRERYGYAGDAHVVIPCTLGMDVRSAPIPNDRDAPVRLVYSGST
                     AGWQSFDLLKGTLTRVLDAQPDVQVLFLSKRDVNNSTLEAAYPGRVQVKWLDHAQVSA
                     ALAQCDLGIMVRERTITNQVASPTKFAEYLASGLRIITNPGLGDFSELVAREDLGIVV
                     APDGPMPPLRATSGAERERLHRYALTHFTKEAHDASYRTVLNALA"
BASE COUNT          265 a          504 c          545 g          335 t
ORIGIN      
        1 gtcaacgaat gggatgcggg gcttgccacg gctctggttc ttgcaagggt gcgatccgcc
       61 gtgacctcgt cacacagggt ctcgttgtgg gcctcaattt tacgtggttc gctcattttc
      121 accaaacagt acctcgctgg ggagatcact gctgcgggca tggtggccat ggcaagcctc
      181 gtgttcatgg tcgttgtgct gtggtcgttc ttgcccggcc tgcggccgtg gatcaacccg
      241 tcaccggtgc tctttgtagc tgtgcgtcgt gtcgttccgc gcggatcgtg ggagacctgg
      301 gacgtgcgcg ccgcacgtgc gatcaccgcc cgacccctca tgacggccag ccgatgacca
      361 acgtgttccg gaggtcgcca agtcgatccc tgcatgttcc cccgtgctgc attcctttgc
      421 ccggtgatcc tctatctcac gtacaacgac cagcccagcg gcgtctattg gagccaggtg
      481 accgatgtgg tggagcacat gaattcgctg ggtggccccg aggtgcgact ggtggccctg
      541 gtgagtgggc ggaatttttt gaggacacgt caggcgatca aggcccacag cccatcgtcc
      601 ctggtactgc ccatggtgcc tgccatgcgg cgttggcgga tgaatacgac cctgctcgcg
      661 tgggtgtgcc gagtgctgcg gccctccggg atcatctgcc gcggtccttt cgccacgtgg
      721 atggctttgc gcatgcgcga tcgtgggtta acggaacgcg tgtgcttcga tggccgcggg
      781 gcctatgcgg cggagtggga ggagtaccgc atcattgaca acgatgcgct gatcgcccag
      841 gtccggccgt tggaacagga ggcggtgggt gcgagcgact tccggctttc cgtcagcaat
      901 gcgctggtgg cccactggcg ggagcgctat ggatacgcgg gtgatgccca cgtggtgatc
      961 ccctgcacct tgggaatgga tgtgcgatcc gctccgatac cgaacgatcg ggatgccccc
     1021 gtgcgactgg tgtacagcgg cagcaccgcc ggttggcagt cgttcgatct gttgaagggg
     1081 acgctcaccc gggtcctgga tgcccagcct gatgtacaag tgctgttcct gagcaagcgg
     1141 gacgtgaaca acagcacgtt ggaagccgcc tatccgggtc gtgtgcaggt gaagtggctg
     1201 gatcacgcac aggtttccgc agcgctcgcg cagtgcgatc tcggcatcat ggtacgcgag
     1261 cggacgatca ccaatcaggt ggccagtcct acgaagttcg ccgagtacct ggccagtggt
     1321 ctgcgcatca tcaccaaccc gggcctcggg gacttcagcg agctggtggc cagggaggat
     1381 ctcggcatcg tggtggcgcc cgatggaccg atgcccccgc ttcgggccac cagcggagcc
     1441 gagcgggaac ggctacaccg gtacgccttg acgcatttca ccaaagaggc gcacgatgcc
     1501 agctaccgaa cggtgttgaa cgccttggcc tgagccgtcc ttgttcccca tggccctccc
     1561 gccgcgttca tgctcgtcag gaactccggc ccgcagcaac gctggtcgca tgaccggtat
     1621 cgacgcggtg agccgggttc accccatac
//
LOCUS       DAOQOC010000789         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56692_length_1647_cov_1.660176, whole genome shotgun sequence.
ACCESSION   DAOQOC010000789 DAOQOC010000000
VERSION     DAOQOC010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56692_length_1647_cov_1.660176"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="fahA"
                     /locus_tag="PL106_11805"
     CDS             complement(<1..1189)
                     /gene="fahA"
                     /locus_tag="PL106_11805"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNA33798.1"
                     /translation="MNIPANSTELRSWIPVPAKSDFPVQNIPFGVGGADGSDSNCLTR
                     IGDSAIDLAILAEQGLIHAPEHLGGKGAFTGELDALLMDGPQVIRDLRQRLSELLRDD
                     ATLNGMHRDAIARAIIPVNELDMELPALIGDYTDFYSSRQHAYNVGVMFRDPANALMP
                     NWLHLPVGYHGRSSSIVVSGTPVRRPMGQFKPAPDQAPVFGPSRQLDLELEVGFLTYE
                     GPELGSRISVEEAEDHIFGLVLFNDWSARDIQAWEYVPLGPFLGKNFASSMSPWVVTL
                     DALEPFRVQGPVQEPQPLPYLRSSGARTFDIRLEVGLTPAGGSETIICRSNFKHLYWS
                     MAQQLAHHTVNGCNVRAGDLMASGTISGDTPDSFGSMLELAWKGTRPIKLNDGSERKF
                     LQDG"
     gene            1292..>1647
                     /locus_tag="PL106_11810"
     CDS             1292..>1647
                     /locus_tag="PL106_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33799.1"
                     /translation="MANGPPKWLIPAILLAHFGLLASYTFPSRWIPDRLRYWSITYAR
                     PLFHQGWDLFAPDPPRCSCEVQAGIGESWRALDASGDNYLRTRMVRNVAAYLNGGSPF
                     PDTLTVPPVMEEALLGL"
BASE COUNT          277 a          529 c          522 g          319 t
ORIGIN      
        1 ccccgtcctg caggaatttc cgttcgctac cgtcgttgag tttgatgggc ctggtgcctt
       61 tccacgccag ttccagcatg ctgccgaagc tgtccggcgt gtcgccgctg atcgtgccac
      121 tggccatcag atcccccgcg cgcacgttgc agccgttcac cgtgtggtgc gcgagttgct
      181 gcgccatgct ccagtacagg tgcttgaagt tgctccggca aatgatcgtt tcacttccac
      241 ctgcgggcgt aaggcccact tccaaccgga tatcgaacgt gcgagcaccg ctggaacgca
      301 ggtagggcag gggttgcggt tcttgcacgg ggccttgcac gcggaaaggt tccagtgcat
      361 ccagcgtgac cacccatggc gacatggagg aggcgaagtt cttgcccagg aacgggccca
      421 gcggcacata ttcccacgcc tggatgtcgc gcgcgctcca atcgttgaag agcaccagcc
      481 cgaagatgtg gtcctcggcc tcctcgacgc tgatgcgact gcccagttcg gggccctcgt
      541 acgtgaggaa gcccacctcc aattcgagat ccagctgccg cgatgggccg aacaccggag
      601 cctggtccgg agcgggtttg aactggccca tgggacggcg cacgggcgtg ccactgacca
      661 cgatgctgct gctgcggcca tgatagccca ccggcaggtg cagccagttg ggcatcagtg
      721 cgttcgccgg gtcgcggaac atcacgccca cgttgtaggc gtgttggcgg ctgctgtaga
      781 aatcggtgta gtcgccgatg agcgcgggca attccatgtc cagctcgttc accggaatga
      841 tggcccgcgc gatcgcatcg cgatgcatac cgttgagcgt cgcatcgtcg cgcaacagtt
      901 ctgaaagacg ttgccgcagg tcgcggatca cctgcgggcc atccatcaac agggcgtcga
      961 gctcgcccgt gaaggcgcct ttgccgccca ggtgctcggg tgcgtggatc agtccctgct
     1021 ccgccaggat cgcgaggtcg atcgcgctgt cgccgatccg cgtgaggcag ttactgtcgc
     1081 tgccatcggc accacccacg ccgaatggga tgttctggac aggaaagtcg ctcttggccg
     1141 gcacggggat ccagctgcgc agttcggtgg agttggcggg tatgttcatg cgatggggcc
     1201 acgaggatac gggcggccgc aaaggtgcga tcggcagcgg atcaatggtc cgggttgtca
     1261 ccggaacgat cgcccgctac cttggccgct catggccaac ggaccaccca agtggctgat
     1321 cccagcgatc ctgctcgcgc atttcggcct tttggcgtcc tacacgttcc catcccgctg
     1381 gataccggac cggctgcgtt actggtccat cacgtacgca cgaccgctgt tccatcaggg
     1441 ttgggacctt ttcgccccgg atcccccacg ctgctcatgt gaagtgcagg caggtatcgg
     1501 cgagagctgg cgcgcactgg acgcatccgg cgacaactac ctgcgaacgc gtatggtccg
     1561 caacgttgcg gcctacctga acggaggttc ccctttcccg gacacgctta ccgtaccacc
     1621 ggtcatggag gaggccctgc tcggtct
//
LOCUS       DAOQOC010000790         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56729_length_1646_cov_3.067253, whole genome shotgun sequence.
ACCESSION   DAOQOC010000790 DAOQOC010000000
VERSION     DAOQOC010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56729_length_1646_cov_3.067253"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..>1646
                     /gene="asnB"
                     /locus_tag="PL106_11815"
     CDS             134..>1646
                     /gene="asnB"
                     /locus_tag="PL106_11815"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNA33800.1"
                     /translation="MGTAWCRVGRQGDNMCGIAGSYQIDLARPATGERIHAALRRIAH
                     RGPDDVGVYEQGRAILGQRRLSIFDTSSAGHQPFTDAGGHFTIVFNGEVFNFRELRAG
                     LEAQGHTFRSQSDTEVVLRLYSLKGEAFLHDLNGFFALAIHDERTDTLFLARDRYGVK
                     PLLWSEADGRFLFASELRALLAMGAAREPDALSAQQYFAYHYIPAPWTVFKGTHKLLP
                     GHCLHVGPGGVRTERWYDLVAEVKKIPKSTDPKKALFGLMDDAVRLRLVADVPVGAFL
                     SGGVDSSIVSALAVRHHEHLHTFSIGFADDPQFDESRYAEEVARHIGSEHHTFRLTRD
                     DLAENYTRLLAATDEPFADSSALPSFMLCERTRKHVTVAVSGDGADEVFGGYTKHQAE
                     LRWCRPGAAERAVRLLAPLWRSLPRSRGGPWSNRFRQYDRFARLATMSPARRFLEMAA
                     FTTHEEADALVAAKADHAAMEARDTRFTEAFQRMADMNGVLLADVQSTLPNDML"
BASE COUNT          287 a          538 c          552 g          269 t
ORIGIN      
        1 agaccacggg ccacgagatc atcaagaccg tgcatccgca ccccaccatg agcgaggcca
       61 tcatggaggc ggccgctgcg gcgtacggcg aggtgatcca tctgtagggg cgacgatccc
      121 gtcgccccta cagatgggca ccgcctggtg ccgcgtggga cgacagggcg acaacatgtg
      181 cggcatagcc ggatcatatc agatcgacct tgcacgtccc gccacgggcg aacgcatcca
      241 cgcggccctg cgccgcatcg cgcaccgggg tcccgatgac gtgggcgtgt acgagcaggg
      301 ccgtgcgatc ctgggccagc gtcgcctgag cattttcgac accagcagcg ccgggcacca
      361 gccgttcacc gatgcgggcg ggcacttcac catcgttttc aacggcgagg tcttcaactt
      421 ccgcgaactg cgggccggtt tggaggccca gggccacacc ttccgcagcc aaagcgatac
      481 cgaggtggtg ctgcggttgt acagcctgaa gggggaggca ttcctgcacg acctcaacgg
      541 cttcttcgcc ttggccattc atgatgagcg gacagatacc ctgttcctgg cccgtgaccg
      601 ctacggagtg aaaccgctgc tgtggagcga agcggacggc cgcttcctgt tcgccagcga
      661 gctgcgggcc ctgctggcca tgggtgcggc ccgcgaaccg gacgcgctga gtgcgcagca
      721 atacttcgcc taccactaca tccccgcgcc gtggacggtg ttcaagggca cgcacaaact
      781 gctgcccggg cattgcctgc atgtgggacc gggcggtgtg cgcacggagc gctggtacga
      841 cctggtggcc gaggtgaaaa agatcccgaa gagcaccgac ccgaagaagg cgttgttcgg
      901 gctgatggac gacgcggtgc gcctgcgact ggtggccgac gtgccggtgg gcgccttcct
      961 gagcgggggc gtggacagca gcatcgtcag tgccttggcc gtacggcacc acgagcacct
     1021 gcacaccttc agcatcggct tcgcggacga tccgcagttc gatgagagcc gctacgccga
     1081 ggaggtggca cggcacatcg gcagcgagca tcacaccttc cggctcacgc gcgacgatct
     1141 ggccgagaat tatacccgtt tactggcggc caccgatgag ccgttcgccg acagcagcgc
     1201 cttgccctcg ttcatgctgt gtgagcgcac gcgcaagcac gtcaccgtgg ccgtcagtgg
     1261 cgatggtgcc gacgaggtgt tcgggggcta caccaagcac caggccgagc tgcgctggtg
     1321 ccggccagga gcggccgagc gggccgtgcg gttgttggcc ccgttgtggc gcagcttgcc
     1381 acggtcgcgt ggcgggccat ggtccaaccg cttccgccag tacgatcggt tcgcgcgttt
     1441 ggccaccatg tcaccggccc gtcggttcct ggagatggcc gccttcacca cgcatgaaga
     1501 agccgatgcg ttggtggcag ccaaggccga tcacgcggct atggaagcac gtgatacgcg
     1561 gttcaccgag gcatttcagc gcatggccga catgaacggc gtgctcctgg ccgatgtgca
     1621 gagcaccctg cccaacgaca tgctgc
//
LOCUS       DAOQOC010000791         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56741_length_1646_cov_2.634821, whole genome shotgun sequence.
ACCESSION   DAOQOC010000791 DAOQOC010000000
VERSION     DAOQOC010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56741_length_1646_cov_2.634821"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PL106_11820"
     CDS             <1..389
                     /locus_tag="PL106_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33801.1"
                     /translation="YTDSLMFTLYRENERMADITPARHKNGFILELDVNSYYTVFVEK
                     NGYRSKSMTFDTHLPEGRMKYKAYKCTINLEAMEKFEHSDPWYLDFPSALVRWDEQKK
                     AFTHSTDYLADIQTKMALLGAQIDPK"
     gene            595..987
                     /locus_tag="PL106_11825"
     CDS             595..987
                     /locus_tag="PL106_11825"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33802.1"
                     /translation="MRQLPLNLPVVGLFLLLTGCGPHRHVASSHPNGKPEVVIYLKGK
                     GEEAEKVMEKVYYPNGQLEYVGHFENGVEQGEWTYYYESGIKKFVEQWDKGLEHGIHY
                     DYSPDGRVYRELHYDHGKLMQEIDRSKQ"
     gene            1057..>1646
                     /locus_tag="PL106_11830"
     CDS             1057..>1646
                     /locus_tag="PL106_11830"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA33803.1"
                     /translation="MSRRIPVTIGALFVGGLLRSQSLYFPPVQTNQPWETVDPGSLGW
                     CMDQLPPLLDLLEENHSKAFIVLKDGRIAIEQYFGMFTQDSVWYWASAGKSLTSVLVG
                     IAQQEGLLDIQAASSTYLGTGWTNCTPAQEAAITVRHQLSMTTGLDDGVPDTDCTDPA
                     CLQYLADPGTRWSYYNAPYTRLDGVISGASGQSIND"
BASE COUNT          369 a          491 c          484 g          302 t
ORIGIN      
        1 gctacaccga cagtctgatg ttcacgctgt acagggagaa tgagcgcatg gctgacatca
       61 cgccggccag gcacaagaac ggcttcatcc tggaattgga tgtgaacagc tattacacgg
      121 tgttcgtcga aaaaaacggc taccgctcca agtccatgac cttcgacacg cacctgcccg
      181 agggccgtat gaaatacaag gcgtacaagt gcacgatcaa cctggaggcc atggagaagt
      241 tcgagcactc ggacccctgg tatcttgatt tcccgagcgc ccttgtgcgt tgggacgaac
      301 agaagaaagc gttcacgcat agcacggact acctggccga catccagacc aagatggcgc
      361 tgctgggtgc acagatcgac cccaaatgac acggcgcctc cggtagctgc caccggaagg
      421 gccagaacag aacggacacc cggggtgtcc gttctgtttt tccgggcacc tattcagccc
      481 cgctccccac ctgccccaca ggcgccgcga tagcgcttcc gatcgcacgg atccgcggcc
      541 gcatcgatgg tcaacctctc gctgttccat gcatcgcact tacattcgac cgcgatgcgg
      601 cagctccctc tcaacctccc ggtggtcggc ttgttcctgc tcttgacggg gtgtggcccc
      661 catcgccatg tggccagcag tcatcccaac ggcaaaccgg aggtggtcat ctatctgaaa
      721 ggcaaaggcg aggaggcgga aaaagtgatg gagaaggtgt attatcccaa cggccagctg
      781 gaatacgtgg ggcacttcga gaacggggtg gaacagggcg aatggaccta ctactacgag
      841 agcggcatca agaaattcgt ggaacagtgg gacaagggcc tggagcacgg gatccattac
      901 gattactccc ccgatgggcg cgtgtaccgg gaactgcatt atgatcacgg caaattgatg
      961 caggagatcg accggtcgaa acagtgaccg ggccgctctg cgcatgatgt tcccttgccc
     1021 gatcggacca aagggcggaa cccgggtcca acgcacatga gcagaaggat cccagtcacc
     1081 atcggtgcct tgttcgttgg cggtttgctc cgctcacagt ccctgtattt cccaccggtt
     1141 cagaccaacc aaccttggga gaccgttgac cccggatcac tcggatggtg catggaccag
     1201 ctgcccccgc tgctggacct cctggaagag aaccatagca aggccttcat cgtgttgaag
     1261 gatggtcgca tcgcgatcga gcagtatttc ggcatgttca cgcaggacag cgtgtggtac
     1321 tgggccagcg caggcaagtc gctcacctcc gtgctggtgg gcatcgcgca gcaggaaggc
     1381 ttgttggaca tccaagcggc gagcagcaca tatctgggca ccggctggac caactgcaca
     1441 ccagcgcaag aagcggcgat cacggtacgc catcaactgt ccatgacgac cggactggac
     1501 gacggcgtgc ccgatacgga ttgcactgac cccgcgtgcc tgcagtatct ggcggatccc
     1561 ggaacgcgct ggagctatta taacgcgcca tacacccggt tggatggtgt gatcagcggc
     1621 gccagtggtc agtcgatcaa cgattt
//
LOCUS       DAOQOC010000792         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56790_length_1645_cov_3.472327, whole genome shotgun sequence.
ACCESSION   DAOQOC010000792 DAOQOC010000000
VERSION     DAOQOC010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56790_length_1645_cov_3.472327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PL106_11835"
     CDS             <1..1224
                     /locus_tag="PL106_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNA33804.1"
                     /translation="DVQRRDPFADFFWGYRQAQPQTQIQRGAGSGVIMSDDGYIVTNN
                     HVVEGADRIMVHLNDKRQFQATVIGRDPSTDIALIKIDAHDMPTLSYGNSDEVRVGQW
                     VLAVGNPMNLTSTVTAGIVSAKARSINILQYDPSRDVFPIESFIQTDAAVNPGNSGGA
                     LVDANGSLIGINSAIASTTGAYTGYSFAIPANIVRKVAADLMEFGSVQRAYLGVSIAD
                     IGPDQAKEIGLDRPKGVYVKGLSQGGAAENAGMKTGDVIVKVGNITVDNVPQLQEQVG
                     KFRPGDAVAVTVVRDGREQVMDMTLRGREGNTSVEAGAKAASRSVLGAEMVPATAEEL
                     KALHLESGVKVVNIIGGKLRSTGIREGFIITRIDREWMREPKDVLRALDEKRGGVLIE
                     GVYPNGTKAYYGLGV"
     gene            1402..>1645
                     /locus_tag="PL106_11840"
     CDS             1402..>1645
                     /locus_tag="PL106_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HNA33805.1"
                     /translation="MRQLKITKSITNRESQSLDKYLQEIGKEGLINAEMEVELARRIK
                     QGDNTALERLVKANLRFVVSVAKQYQNQGLSLPDLIN"
BASE COUNT          375 a          479 c          515 g          276 t
ORIGIN      
        1 gatgtgcaac gtcgcgatcc tttcgccgac ttcttctggg gttaccgtca ggcacagccg
       61 caaacccaga tccagcgcgg tgcgggtagc ggcgtgatca tgagcgacga cggctacatc
      121 gtgaccaaca accacgtggt ggaaggtgcg gaccgcatca tggtccattt gaatgacaag
      181 cgacagttcc aggccaccgt gatcggtcgc gaccccagta cggacatcgc cctgatcaaa
      241 attgatgcgc acgatatgcc gacgctctca tacggcaata gcgatgaggt gcgggtggga
      301 caatgggtgc tggccgtggg caaccccatg aaccttacca gcacggtgac cgccgggatc
      361 gtgagcgcca aagcgcgcag catcaacatc ctgcagtacg accccagccg cgatgtgttc
      421 ccgatcgaga gctttatcca gaccgatgcg gcggtgaatc ccgggaacag cgggggtgct
      481 ctggtggacg ccaatggcag ccttatcggc atcaatagcg ccatcgccag caccaccgga
      541 gcgtatacgg gctattcctt cgccataccc gccaatatcg tgcgcaaggt ggccgccgac
      601 ctgatggagt tcggcagtgt gcaacgggct tatctcgggg tgagtatcgc tgacatcggc
      661 ccggaccaag ccaaggagat cgggctggac cgacccaaag gcgtgtacgt caagggcttg
      721 agccaggggg gtgctgccga aaatgcgggg atgaagaccg gtgacgtgat cgtgaaagtg
      781 ggcaacatca ccgtggacaa tgtgccgcag ttgcaggaac aagtgggcaa gttccggccc
      841 ggcgatgccg tggctgtaac cgtggtgcgc gatggccgcg aacaggtgat ggacatgacg
      901 ctgcggggcc gcgaagggaa cacctcggtg gaggccggcg caaaggctgc ttccagaagc
      961 gtactgggcg ccgaaatggt tccggccaca gccgaggaac tcaaggccct gcacttggaa
     1021 agtggggtga aagtggtgaa tatcatcggc ggaaagctcc ggagcaccgg tatccgcgaa
     1081 ggattcatca tcactcggat cgatcgtgaa tggatgcgcg aaccgaagga tgttcttcgt
     1141 gccctcgatg agaaacgtgg tggtgtactc atcgaagggg tttaccccaa cggcacgaag
     1201 gcctactacg ggctcggggt ctgaaaagcc ccgtccagcg cgtattccgc tccagggtgc
     1261 gccccccgat cggccggctt gttcggtcag accggggggc cgtaccttgg ccgccggatc
     1321 agcaggtgac cacctcgccc gctggcccac cctaccaccg atccatacac accaaatacc
     1381 ctgccgcccg tgacaagcag gatgcgccaa ctgaagatca ccaagtcgat caccaaccgg
     1441 gaaagtcaat cactggacaa atacctgcag gagatcggta aagaaggact gatcaatgcg
     1501 gaaatggagg ttgaactggc gcgccgcatc aagcaaggcg acaacaccgc gctggagcga
     1561 ctcgtgaagg ccaatctgcg tttcgtggtt tccgtggcca aacaatacca gaatcaaggt
     1621 ctcagcctcc ccgacctgat caacg
//
LOCUS       DAOQOC010000793         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56817_length_1645_cov_2.558491, whole genome shotgun sequence.
ACCESSION   DAOQOC010000793 DAOQOC010000000
VERSION     DAOQOC010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56817_length_1645_cov_2.558491"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1645)
                     /locus_tag="PL106_11845"
     CDS             complement(<1..>1645)
                     /locus_tag="PL106_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33806.1"
                     /translation="LGNDHVSDVKVDESGAIYITGEFSGDADFTDSVFTSSGALDCFV
                     AKMTPEGQITWFVRGGGLGIDRGIRLAFGPDNTLAVVGEFQGVAEFQGTSITSQAYTP
                     DMFCMVLDRATGALHWMRHGGGTLGSDRPYGVTVSASGQVTMAGEFKGTATWDGSILV
                     STPDQDTGEPTMDVVVISYSDNGTLLWAKQGVADREDRALGVVNDDAGNIYVAGQFSD
                     TITFDATHLNAMYNATFLLKLDGTGNEQWFRRIGGGIYGHLRDMLAVPGGGLLLLGDL
                     QGTMLFMDDAPDIIAGEASYNYYLLQVDAAGQLAAQGQIGSSNGLSARGLDLRNDTIS
                     VIGQFNCRFTALGDHYGGSGLFMAAGEEDIFLARHRYNDLGFIEARHFGGSGRKLPGR
                     VASLPNGDVLACGSYERSIAFATTTANVVSGDDFIGYVPTPTPPNPSGCPDLANKTFR
                     AQDAMGLMDGFIARAYVRDSVVFDWWRQLPGACDHPATWSMCVGQAAFGQPCPDTFTF
                     CGPGTIGATLPFIPAFGNGNSIGPLVTAVWTSGDSTLSIS"
BASE COUNT          300 a          520 c          537 g          288 t
ORIGIN      
        1 cactgatact cagcgtggag tcgccgcttg tccacacggc tgtcacgagc ggaccgatgg
       61 agtttccgtt cccgaaagcg ggtatgaatg ggagtgtggc accgatggtg cccgggccac
      121 agaatgtgaa cgtatccggg cagggctggc cgaatgcggc ctggccgacg cacatgctcc
      181 aggtggcggg gtggtcacag gcaccgggca actggcgcca ccagtcgaag accacactgt
      241 cccgtacata ggcacgggcg atgaaaccgt ccatcaggcc cattgcatcc tgggctcgga
      301 aggtcttgtt ggccagatcc gggcagccgg aagggttcgg gggtgtgggc gtgggcacat
      361 acccgatgaa gtcgtcaccg gacacaacat tcgccgtggt ggtggcgaag gcgatactgc
      421 gttcatagga accgcaggcg agcacgtcgc cattggggag ggaggccacg cgcccgggca
      481 gcttgcgacc cgagcccccg aagtgccggg cctcgatgaa gccgaggtca ttgtaccgat
      541 gacgggcaag gaagatgtcc tcctcccctg ccgccatgaa gagccctgag ccgccgtagt
      601 ggtcacccag tgcggtaaag cggcaattga actggccgat caccgagatg gtgtcgttgc
      661 gcaggtcgag gccacgcgca ctgaggccgt tggaggagcc gatctggccc tgcgcggcga
      721 gctgcccggc cgcatccacc tggaggaggt agtaattgta ggatgcctcc ccagcgatga
      781 tgtccggcgc gtcgtccatg aagagcatgg tgccctgcag gtcgcccagc aggagcagac
      841 ctccgcccgg cacggccagc atgtcgcgca ggtgaccgta tatgcccccg ccgatgcgcc
      901 ggaaccattg ttcgttgcca gtgccgtcca gcttcagcag gaaggtggcg ttgtacatgg
      961 cgttcaggtg ggtggcatcg aaggtgatgg tatcgctgaa ctgtccggcc acgtagatgt
     1021 ttcccgcatc gtcattcacc acgccgagcg cccggtcctc ccggtcggcc acaccctgct
     1081 tcgcccagag caacgtaccg ttatcactgt agcttatgac aaccacgtcc atggtgggct
     1141 cgcccgtgtc ctggtcgggg gtgctcacga ggatgctacc atcccaggtg gccgtgccct
     1201 tgaattcacc ggccatggtc acctgcccac tggcagaaac ggtgacgccg tacggcctgt
     1261 cggatcccag tgtcccgccg ccgtgcctca tccagtgcag cgcgccggtg gcacggtcca
     1321 acaccatgca gaacatgtcg ggcgtgtagg cctgggaagt gatcgatgtg ccttggaatt
     1381 cggccacgcc ctggaactct cctaccacgg ccagggtatt gtccggaccg aaggccaggc
     1441 ggatgccccg gtcgatgccg agcccgccac cccggacgaa ccaggtgatc tggccctcgg
     1501 gcgtcatctt cgccacaaag caatcgagcg cgccactgga ggtgaagacc gaatccgtga
     1561 agtcggcgtc gccgctgaat tcgccggtga tgtagatggc accgctctcg tccaccttca
     1621 catcgctcac gtggtcgttg cccag
//
LOCUS       DAOQOC010000794         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56862_length_1644_cov_3.365009, whole genome shotgun sequence.
ACCESSION   DAOQOC010000794 DAOQOC010000000
VERSION     DAOQOC010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56862_length_1644_cov_3.365009"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PL106_11850"
     CDS             complement(<1..574)
                     /locus_tag="PL106_11850"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 domain-containing protein"
                     /protein_id="HNA33807.1"
                     /translation="MGALLNEAYDRYARPDFITDDPIQVPRAFARREDAEVIGFLTAS
                     IAWGLRRTIIANAWKLVRLMDERPHDFVMNASTSELEHLGAFVHRTFNGTDLRHFILA
                     SRHLYHGYGSLEAAFLEDGTFGDARQGITRFRERFFAIAHASRTRKHVADPGRGSHAK
                     RINMFLRWMVRPDDRGVDLGLWDRIAPADLM"
     gene            complement(606..1199)
                     /locus_tag="PL106_11855"
     CDS             complement(606..1199)
                     /locus_tag="PL106_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33808.1"
                     /translation="MVMRFAGALLYLTALSAIGQESTYKGVPVAEPLLVRGSVAISPG
                     FMLQHPVTNIYVVGKLEVFTDKRISLRGEGLWYIDSQQRTPVLQQNSQLAFGPFYHFT
                     SKRLDLCIGFEPGVAFSRPVRDTTFLEPAPLRAIPNMALCAGLTYSIWDYFQFFVDAR
                     YVHARYTGPYSNGLPLDEVLLGAGLGWQVPVKRLIKK"
     gene            complement(1193..>1644)
                     /locus_tag="PL106_11860"
     CDS             complement(1193..>1644)
                     /locus_tag="PL106_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33809.1"
                     /translation="RVRISERVNTDWFYDYFTGPSGSLAHREDQHIGWSVLFYLRAHT
                     QERQLLQPYVVAGHCFDYTKQRSNRDPSNFAERWSSAVQAGVGNHFNVTDRADLSLVC
                     QYMWHLGTDIHAEEHAGQVHFHKEKGANLEGHLLIHVSFNYKLFDAW"
BASE COUNT          309 a          515 c          518 g          302 t
ORIGIN      
        1 ccatcaggtc ggccggggct atgcgatccc acagcccaag gtccacaccc cggtcgtccg
       61 ggcggaccat ccaccggagg aacatgttga tgcgcttcgc atggctgccg cgcccggggt
      121 cggccacgtg cttgcgtgtg cgcgacgcgt gcgcgatcgc gaaaaaacgc tccctgaaac
      181 gcgtgatccc ctgccttgcg tcaccgaatg tgccgtcctc caggaaggcc gcttccagac
      241 ttccgtaccc atggtacaga tggcgcgagg ccaggatgaa gtgccgcagg tccgttccgt
      301 tgaacgtgcg atgcacgaag gcgcccaggt gttccagttc gctggtcgag gcgttcatca
      361 cgaagtcatg cggccgctcg tccatcagcc ggaccagctt ccatgcattg gcgatgatcg
      421 ttcggcgcag gccccaggcg atgctggccg tgaggaagcc gatcacttcc gcatcctcac
      481 gacgagcgaa ggccctcggc acctggatcg gatcgtcggt gatgaaatcg ggccgcgcgt
      541 accggtcgta cgcctcgttg agcagggctc ccacatcttc ggatggagcg cgcttcaccg
      601 gcggatcact tcttgatcaa ccgtttcacc ggcacctgcc agccgaggcc ggcacccagc
      661 agcacctcat ccaagggcag gccattgctg tacggaccgg tgtacctggc atgcacgtac
      721 cgcgcatcaa cgaagaactg gaagtagtcc cagatggagt acgtgagtcc ggcgcacaag
      781 gccatgttcg ggatggcccg tagtggcgcg ggttcgagga aggtggtgtc ccgcaccggt
      841 cgcgagaacg ccacacccgg ctcgaaaccg atacacagat ccagccgctt actggtgaaa
      901 tggtagaatg ggccgaaggc gagctggctg ttctgttgca gtaccggcgt gcgttgctgg
      961 ctgtcgatgt accacagccc ttccccacga agactgatgc gcttgtcggt gaagacctcg
     1021 agcttgccga ccacgtaaat gttggtgacc gggtgctgga gcatgaagcc ggggctgatg
     1081 gccactgaac cgcgcacgag caggggctcc gccacaggga ctcccttgta tgtgctctcc
     1141 tgcccgatcg ccgaaagggc ggtcaggtac aacagggcac cagcgaaccg catcaccatg
     1201 cgtcgaagag cttgtagttg aaactcacat ggatcaagag gtgcccttcc aggttggcgc
     1261 ctttttcctt gtggaagtgg acctgtccag cgtgttcctc ggcatggatg tccgtaccga
     1321 ggtgccacat gtactggcac accaaggaaa gatccgcgcg atcggtgacg ttgaagtggt
     1381 tgcccacgcc cgcttgcacc gcgctgctcc atcgctcggc gaaattcgag gggtcccggt
     1441 tcgaccgctg cttggtgtag tcgaagcaat ggcccgcgac cacgtaaggc tgcaggagtt
     1501 gccgctcctg tgtgtgggca cgcaggtaga acagcacgct ccacccgatg tgctggtcct
     1561 cccggtgcgc gagcgaaccc gacggacccg tgaagtagtc gtagaaccag tcggtgttca
     1621 cccgctcgct gatgcgcacg cgca
//
LOCUS       DAOQOC010000795         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56918_length_1643_cov_5.195214, whole genome shotgun sequence.
ACCESSION   DAOQOC010000795 DAOQOC010000000
VERSION     DAOQOC010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56918_length_1643_cov_5.195214"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PL106_11865"
     CDS             complement(<1..1489)
                     /locus_tag="PL106_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNA33810.1"
                     /translation="MSDAIKHECGLAFIRLRKPLRHYQQKYGTAFHGINKLFLLMEKQ
                     HNRGQDGAGVGVIKLDPKPGTNYIDRIRSTDKQAIQKVFRDIHRGIQEVVEKDPAIAS
                     DVDRLKTQAPFLGEVLLGHLRYATYGSGELENCHPRRRLSNWITRNLVLAGNFNMTNV
                     DELFDLLVGIGQCPKEKSDTMTVLEKIGHYLDVENDRLRQIHAPLGETDAQLTRLIGE
                     QLDVAGILRMSAQDFDGGYAFAGLIGHGDAFVLRDPAGIRPCFWYADEEFVVAASERP
                     AIQTAFAVRTEQVHELRPGHALIVRKDGTFSEEQVRVPVEKRACSFERIYFSRGSDRD
                     VYRERLALGRNTVPAILEAVDNDMRNTVFSFIPNTAEVACYGMLKGLEDELNRTKERM
                     IIDLGPAPEPGKLREILSMRPRMEKVAIKDAKLRTFITADNARDELVAHVYDITYGSV
                     RPGVDNLVVIDDSIVRGTTLRQSILRMLDRLEPKRIVVVSSAPQIR"
BASE COUNT          270 a          549 c          488 g          336 t
ORIGIN      
        1 acctgatctg cggcgcactg ctgaccacca cgatgcgttt gggctccagc cggtccagca
       61 tgcgcaggat gctctgccgc agtgtggtcc cacgaacgat gctgtcatcg atcaccacca
      121 ggttgtccac cccgggccgt acgctcccgt acgtgatgtc gtacacgtgc gccaccagct
      181 cgtcacgggc gttgtcggcg gtgatgaagg tgcgcagttt cgcatccttg atggcgacct
      241 tctccatgcg cggacgcatg gagaggatct cgcgcagctt gccgggctcc ggtgcgggac
      301 ccagatcgat gatcatccgc tccttggtcc ggttcagctc gtcctccaga cccttgagca
      361 tgccgtagca ggccacctcg gcggtgttgg gaatgaagct gaataccgtg ttccgcatat
      421 cgttgtccac cgcttcgagg atggccggga ccgtgttccg ccccagcgcg aggcgctcgc
      481 gatacacatc ccggtcgcta ccgcgactga aatagatacg ttcgaaactg catgcccgtt
      541 tctccacggg cacccgcacc tgttcctcgc tgaacgtacc gtccttgcga acgatgaggg
      601 cgtggccggg ccgcagctcg tgcacctgct cggtcctcac cgcgaaggcc gtctggatgg
      661 cggggcgttc gctggccgcc accacgaact cctcatcggc gtaccagaag catggacgga
      721 tccccgctgg atcgcgcaat acgaaggcgt cgccgtggcc gatgagcccg gcgaaggcgt
      781 aacccccgtc gaaatcctga gcgctcatgc gcaggatacc cgccacgtcc agctgttcgc
      841 cgatgaggcg cgtgagttgc gcatcggttt cgcccagcgg tgcatggatc tgccgtagcc
      901 ggtcgttctc cacatccagg tagtggccga tcttctccag caccgtcatg gtgtcgctct
      961 tctccttggg acattggccg atgcccacga gcaggtcgaa gagctcgtcc acattggtca
     1021 tgttgaaatt gcccgcgagc accaggttgc gcgtgatcca gttgctcagg cgccgtcgtg
     1081 ggtggcaatt ctccaactca ccactaccgt acgtggcgta gcgcaaatgg cccagcagca
     1141 cctcgccaag gaacggggcc tgcgtcttca accgatcgac atcgctggcg atcgcagggt
     1201 ccttctcaac aacctcctgg atgcccctgt gtatatcccg gaagaccttc tggatggcct
     1261 gcttgtccgt gctgcggatg cgatcgatgt agttggtccc cggtttcggg tccagcttga
     1321 tcacgcccac ccccgcgcca tcctgcccgc ggttgtgctg cttctccatc agcaggaaca
     1381 gcttgttgat gccgtggaag gcggtgccgt acttctgctg atagtggcgc agcggtttgc
     1441 gcaagcggat gaaggccagt ccgcattcgt gtttgatggc atcgctcatt tctgacccat
     1501 cgcaacatgc ttgccgatgc ggtgggccgc caaattacgc ccggggcccg cacggcccag
     1561 gaacagcttc ctggcggctc ctccaggtca gggccgatcc cgttcctcgc ccagggcaac
     1621 gccaagcccc catccccggt ctt
//
LOCUS       DAOQOC010000796         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_56934_length_1643_cov_3.647355, whole genome shotgun sequence.
ACCESSION   DAOQOC010000796 DAOQOC010000000
VERSION     DAOQOC010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56934_length_1643_cov_3.647355"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1643)
                     /locus_tag="PL106_11870"
     CDS             complement(<1..>1643)
                     /locus_tag="PL106_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HNA33811.1"
                     /translation="SPDGSTRTFDAQGKGTSFSDGVGIIVLKRLGDAVRDRDHIYATI
                     KGAALNNDGSDKASFTAPSVRGQAEVIAMAQADAGVSPEQITYVEAHGTATPLGDPIE
                     VEALTLAFGGKKDGQHCSIGSIKSNIGHLTAAAGAAGVIKTALALQQEKIPASIGFEK
                     PNPAIDFANSPFRVAQDNTAWPRTGTPRMAGVSSFGVGGTNAHVIMAEPPVMAASSAS
                     RSKQLLMLSAKTKTSLDAMTDNLRAWLEAHPEASLADAAYTLQIGRRHFKHRRLIVGG
                     SPGEVIEAIANKDANLIGTRELHEAAPGVVFMFPGQGSQYVNMGRDLCASEPVFKQHF
                     DRCCELFSKEFGTDLKAIIFPKAGEEEKAAEQLKQTIYTQASLFTMHYSLAKLWMHWG
                     ITPDAMMGHSIGEFAAACLAGVFSLEDAVKLVANRGRMMQELPGGSMLSVRAAEEDVA
                     KKLPSGCSIAANNGPQLCVASGPHEAIAKLQADLEKDGITCKLLVTSHAFHSPMMDAM
                     VAPYRKVVESVKPSAPRIPIVSTVTAEWLKDDEATSPKY"
BASE COUNT          269 a          517 c          503 g          354 t
ORIGIN      
        1 caatacttcg gtgaagtggc ttcatcgtcc ttcagccatt cggccgtgac ggtgctcacg
       61 atcgggatgc gcggtgcgct cggcttcacg ctctccacca ccttcctgta cggcgccacc
      121 atggcgtcca tcatcgggct gtggaaggcg tggctggtca cgagcaactt gcaggtgatg
      181 ccgtccttct ccaaatcggc ctgcagcttg gcgatcgcct catgcggacc gctggccacg
      241 cacaactgcg gaccgttgtt cgccgcgatg ctgcaaccac tgggaagttt cttggccaca
      301 tcctcttccg ccgcgcgcac gctgagcatg ctgccaccgg gcagttcctg catcatgcgg
      361 ccgcggttgg ccaccagctt cacggcatct tccaacgaga acacaccggc caggcaggct
      421 gcggcgaatt caccgatgct atggcccatc atcgcatccg gtgtgatgcc ccaatgcatc
      481 cacagcttcg caaggctgta gtgcatggtg aacagcgagg cctgcgtgta gatggtctgc
      541 ttcagttgct cggccgcctt ttcctcttca ccggctttcg ggaagatgat ggccttgagg
      601 tcggtaccga actccttgct gaagagctcg cagcatcggt cgaaatgttg tttgaagacc
      661 ggctcactgg cacagaggtc gcggcccatg ttcacgtatt gcgagccttg tccggggaac
      721 atgaacacca cgcccggagc ggcctcgtgc agttcacgcg tgccgatcag attggcgtcc
      781 ttgttggcga tggcctcgat cacttcgcca gggctgcctc ccacgatcag gcggcgatgc
      841 ttgaagtgcc gacgaccgat ctgcagcgtg taggccgcat cggcaagcga tgcttctgga
      901 tgcgcttcga gccatgcgcg gaggttgtcc gtcatggcat cgaggctggt cttggtcttc
      961 gcactcagca tcagcaattg tttgctgcgc gatgcgctgg aagccgccat caccggaggc
     1021 tctgccatga tcacgtgcgc gttggtgccg cccacgccga agctgcttac gcccgccatg
     1081 cgtggcgtac cggtacgcgg ccaggccgta ttgtcctggg ctacgcggaa gggcgagttc
     1141 gcgaagtcta tggcggggtt cggcttctcg aaaccgatgc tcgcggggat cttttcctgt
     1201 tgaagggcta aagcggtctt tatcacgcca gccgcaccgg ccgcggccgt caggtgaccg
     1261 atgttcgatt tgatggagcc gatcgaacag tgctgaccat ccttctttcc gccgaaggct
     1321 aatgtcaacg cctccacctc aatgggatca ccgagcggcg tggcagtgcc gtgcgcttcc
     1381 acgtacgtga tctgctcggg tgaaacaccc gcatcggcct gcgccatggc aatcacctcg
     1441 gcctgaccac gcacgctggg tgcggtaaag ctggccttgt cactgccgtc gttgttcaac
     1501 gcggcgcctt tgatcgtggc gtagatgtgg tccctgtcgc ggaccgcgtc gccgaggcgc
     1561 ttcaggacga tgatgccgac accatcgctg aagctagtgc ccttgccttg tgcatcgaag
     1621 gtgcgcgtgc tgccgtcggg gct
//
LOCUS       DAOQOC010000797         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_57031_length_1642_cov_3.081916, whole genome shotgun sequence.
ACCESSION   DAOQOC010000797 DAOQOC010000000
VERSION     DAOQOC010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57031_length_1642_cov_3.081916"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..494)
                     /locus_tag="PL106_11875"
     CDS             complement(15..494)
                     /locus_tag="PL106_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNA33812.1"
                     /translation="METTADISKRTLVLTRRLNAPRELVFAAWTDPKHLVRWWGPKDF
                     TLPHCEQDFRVGGAYRFCMRAPDCSDHWVRGEYTHIDPPRRLVFTWLREDNEGDIWCD
                     TVVCITLKEQGGATLLTLEQTTFATVAHCEEHAVGWGECLDRLVRFTEQHGSSTSPL"
     gene            complement(494..955)
                     /locus_tag="PL106_11880"
     CDS             complement(494..955)
                     /locus_tag="PL106_11880"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNA33813.1"
                     /translation="MITTPEVITTHETTTAVIPLLIPCQDMGKYMDPAIQEVIKAITG
                     QGIAIAGPMFSYHKRRPSDSFDFEIGFPVSKAIKAEGRVINGKLPAVKVVRSVYQGPY
                     DNLGQAWGALQKWVREQGYGETGRFWESYLNNPDEVKDPKDYLTELNWIIG"
     gene            complement(1044..>1642)
                     /locus_tag="PL106_11885"
     CDS             complement(1044..>1642)
                     /locus_tag="PL106_11885"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA33814.1"
                     /translation="GTTLMTITARYDSWKTRDEVLASPMEGGLEFSYQELDALLAKKI
                     ASGSKTSSSRTGRKAKPGNALFSGVSEPELVDAYMKTLKHPLKPVAEQLRKVILAADK
                     SIGEGIYWNAPCFYFTGKMEPFDPKTYKRYIVGFNFFKQDTLRLIFLRGADVEKQGGL
                     LTGDYKDGRRLALFSSMADVKKHEKELKRIVKQLIKTM"
BASE COUNT          284 a          503 c          495 g          360 t
ORIGIN      
        1 ctggtcctgg atcgtcatag cggtgatgtg gatgatccgt gttgctcagt gaaacgcacg
       61 aggcggtcca ggcattcgcc ccaaccgacg gcatgttcct cgcaatgcgc cacggtggcg
      121 aaggtggtct gctccaaggt gagcaaggtg gccccgcctt gttccttcag cgtgatgcac
      181 accacggtat cgcaccagat gtcgccctcg ttgtcctcgc gcagccaagt gaagaccagc
      241 cgacgcggcg ggtcgatgtg cgtgtactcg ccgcgcaccc aatggtcgct gcaatccggt
      301 gcgcgcatgc agaagcggta tgcaccgccc acgcggaagt cctgttcgca atgcggcagg
      361 gtgaaatcct tcgggcccca ccagcgtacc aaatgcttgg gatcggtcca tgcggcaaag
      421 accagctcgc gcggggcgtt gagcctgcgg gtgagcacca gggtacgctt cgagatgtcc
      481 gcggtcgttt ccatcagccg atgatccagt tcaactccgt caagtaatcc ttcgggtcct
      541 tcacctcatc gggattgttc aggtagctct cccagaagcg gccggtttcg ccatagcctt
      601 gctcacgcac ccacttctgc aacgcgcccc aagcctgccc caggttgtcg tacggccctt
      661 ggtacacgct gcgcacgact ttcacagccg gcagcttgcc gttgatcacc cgcccttccg
      721 ccttgatcgc cttcgatacc ggaaatccga tctcgaagtc gaagctgtcg ctcgggcgcc
      781 gcttgtggta gctgaacatc ggcccggcga tggcgatgcc ttggccggtg atggccttga
      841 tgacctcctg gatcgcgggg tccatgtatt tgcccatgtc ctggcagggg atcaaaagcg
      901 ggatcacggc agttgtggtc tcatgggtgg taatgacttc gggtgtggtg atcatgggtt
      961 cgatggttgt cgtcggggcg ggaatgctga gcgaagtcga agcacccgcc cttactgagt
     1021 ttggggcgcg agatctcgcg cccttacatg gtcttgatga gttgcttcac gatccgcttc
     1081 agttccttct catgcttctt cacgtcggcc atgctgctga agagcgcgag ccgacgacca
     1141 tccttatagt cgccggtgag caggccgccc tgcttctcca catctgcgcc gcgcaggaag
     1201 atgaggcgta gcgtgtcctg cttgaagaag ttgaagccga cgatgtagcg cttgtaggtc
     1261 ttcgggtcga agggctccat ctttccggtg aagtagaagc acggcgcatt ccagtagatg
     1321 ccctcgccga tcgacttgtc cgcggccagg atcaccttgc gcaactgttc cgcaacgggt
     1381 ttgagcggat gcttcaatgt cttcatgtac gcatccacca gctccggctc ggagacgccg
     1441 gagaacaaag cgttccctgg cttggccttc ctgccggtgc gggacgaaga cgtcttggag
     1501 cctgacgcga tcttcttggc caggagcgca tccaactcct ggtaactgaa ctccagtccg
     1561 ccttccatcg gcgatgcgag cacctcgtcg cgggtcttcc acgagtcgta ccgcgccgtg
     1621 atcgtcatca aggtggtgcc tt
//
LOCUS       DAOQOC010000798         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_57242_length_1639_cov_3.374369, whole genome shotgun sequence.
ACCESSION   DAOQOC010000798 DAOQOC010000000
VERSION     DAOQOC010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57242_length_1639_cov_3.374369"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1639)
                     /locus_tag="PL106_11890"
     CDS             complement(<1..>1639)
                     /locus_tag="PL106_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33815.1"
                     /translation="KSNTESTDMGRIDVDVQKLQTLPALLGEVDILKTIQYLPGVKSN
                     GEGNSGFYVRGGGPDQNLILLDEATVYNASHLFGFFSVFNADAVKNLELIKGGMPANY
                     GGRASSVLDITMKEGNDRGFHGRGGIGLIASRLTLEGPIVKERGSFIVSGRRTYVDVL
                     AKPLINPESTFAGSGYYFYDLNAKANYRLGPKDRVYLSGYFGRDVFDLKGSLAGSPSF
                     RIPWGNATVSTRWNHVFGPKLFMNATAVFSDYRFSFSGGQDQFAFKLSSGIKDIGLKV
                     DLTQYPALRHQLKYGMQYTRHIYTPSTVEVESGDTDFDIDTPSKLHAHEAAVYALDEY
                     DITEDLRVNVGLRFSWFAHIGPFHEYLLGTDGRPDGAVDHGAGKIVKTYSGLEPRLSA
                     RYRIDENSSLKASFNRNMQYVHLASYSSVGLPTDVWIPSGQNVKPQIAMQYATGYFRD
                     LADHTYEASVEVYYKDLFNQIEFAEGATPDEGANTNYDAMLVFGKGWSYGAEFFLKRR
                     LGKLTGWAGYPWSRTMRLSPDVNHGRAFPSRWDRRHDG"
BASE COUNT          276 a          506 c          513 g          344 t
ORIGIN      
        1 ccgtcgtgcc ggcggtccca tctgctgggg aacgcgcggc cgtggttcac atcgggggag
       61 agccgcatgg tcctgctcca ggggtagccc gcccagccgg taagcttccc caaacggcgc
      121 ttcaggaaga actccgcgcc atagctccag cctttgccga agaccagcat cgcgtcgtag
      181 ttggtgttgg cgccttcgtc gggtgtggcc ccttcggcga actcgatctg gttgaacagg
      241 tccttgtaat agacctccac gctggcctcg taggtgtggt cggccaggtc gcggaaatac
      301 ccggtggcat actgcatcgc gatctgcggc ttcacgttct ggccactcgg gatccacacg
      361 tcggtgggca gccccacgct gctgtaactg gccagatgca cgtactgcat gttgcggttg
      421 aagctggcct tcaagctgct gttctcgtcg atccggtagc gggcggaaag gcgtggctcc
      481 agtccgctgt atgtcttcac gatcttgcca gcaccatgat ccaccgcccc gtcgggcctc
      541 ccatcggttc cgagcaggta ctcgtggaag ggcccgatat gggcgaacca cgagaagcgc
      601 aagccgacgt tcacgcgcag atcctcggtg atgtcgtact catccagcgc gtacacggcg
      661 gcttcatgag catggagctt gctgggggtg tcgatatcaa aatccgtatc accgctctcg
      721 acctccaccg tgctgggcgt atagatgtgg cgggtgtact gcatgccgta cttcagttgg
      781 tgccggagcg ccgggtactg ggtgaggtcc accttcaagc cgatgtcctt gatgccgctg
      841 ctcagcttga acgcgaactg gtcctgtcca ccggagaaac tgaagcgata gtcgctgaag
      901 acagcggtgg cgttcatgaa gagtttgggc ccgaaaacgt ggttccagcg ggtgctcacc
      961 gtggcgttgc cccaggggat ccggaagctc ggactgccag ccaggctgcc cttcaggtcg
     1021 aacacgtccc ggccgaagta accgctgagg tacacgcggt ccttcggtcc cagacggtag
     1081 ttggccttgg cgttgaggtc atagaaataa tacccgcttc ccgcgaaggt gctctccggg
     1141 ttgatgaggg gcttggccag cacgtccacg tatgtgcggc gaccactgac gatgaagctg
     1201 ccgcgctcct tgacgatggg cccctccagg gtgaggcggc tggcgatgag accgatgccg
     1261 cctcggccgt ggaagccccg gtcgttgccc tccttcatgg tgatgtccag cacgctgctg
     1321 gcgcgtcctc cgtagttggc gggcatgcca cccttgatca gttcaaggtt cttgaccgcg
     1381 tcggcgttga acacgctgaa gaaaccgaag aggtggctgg cgttgtacac cgtggcctcg
     1441 tccagcagga tcaggttctg gtccggtcca cctccccgca catagaagcc gctgttgccc
     1501 tctccgttgc tcttcacccc gggcaggtac tggatggtct tcaggatatc cacctcgccc
     1561 aggagcgcag gaagcgtctg cagtttctgc acatccacat cgatgcgccc catgtcggta
     1621 ctctcggtgt tgctcttgc
//
LOCUS       DAOQOC010000799         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_57835_length_1631_cov_3.036802, whole genome shotgun sequence.
ACCESSION   DAOQOC010000799 DAOQOC010000000
VERSION     DAOQOC010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57835_length_1631_cov_3.036802"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1631)
                     /locus_tag="PL106_11895"
     CDS             complement(<1..>1631)
                     /locus_tag="PL106_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HNA33816.1"
                     /translation="AQNDASNPGRLRVTGSTPNIGHGALNVRGVDAQGNRWFLCGTDT
                     ISINDPNSSQQFQCPNQQQTAKQLILQRVYHKNGAAMTFYERFAGTMTYHPTHGHNHV
                     DDWATFTLRIQTADPDPRNWPIVGEGAKVGFCLMDYYSCANSSANGHCRTSQEYQGGS
                     PLNQTSQFPNYGHGGGSYNCSQISQGISSGYTDVYSESLDGMWINIPPGTCNGNYWIV
                     MEVDPHNNFLEENDGNNWTAVPFTLTQQVPGGGHFAQISADGPTSFCEGGSVQLTASA
                     GTSHLWSTGATTQSITVTTPGDYSCTVTGNCGTDATPVATVEVANTPQPQGTGATIMG
                     SGSAQISATGTDVHWFDQSVGGSEMGSGNIFQTPVINTTTQYWAENRAGVAGLSGNVG
                     KTDNTGGGGNSSVEQYLTFDALAAFTLKSVKVYAQGAGNRTFQVLSNGGGLIAQTTVN
                     VPNGESRVNLDLAVPQGAGMRLYVTSTMRNLYRSNGGVSFPYTIADLVSITGSSAGAN
                     YYYYAYDWEVEGQSLDCISQRTQVTATVISGVQVN"
BASE COUNT          270 a          491 c          511 g          359 t
ORIGIN      
        1 gttcacctgt acgccgctga tgaccgtggc ggtcacctgg gtgcgctggc tgatgcaatc
       61 caacgactgg ccttccactt cccagtcgta tgcatagtag taataattgg ccccggcgct
      121 cgaccccgtg atggacacga ggtccgcaat ggtgtacggg aagctgacgc caccgttgct
      181 ccggtacaaa ttccgcatgg tgctggtgac atagagacgc atgccggcac cttggggtac
      241 cgcaagatcc aggttcacgc gtgactcgcc gttcggcacg ttgaccgtgg tctgggcgat
      301 gagtccaccg ccgttgctga gcacttggaa ggtgcgattg ccagcgccct gcgcatacac
      361 cttcacactc ttcagggtga aggcggccaa cgcatcgaac gtgaggtatt gctccacgct
      421 gctgttccca ccaccaccgg tattgtccgt tttacccacg ttccccgaca agcctgccac
      481 cccggccctg ttctcggccc agtactgcgt ggtcgtattg atcaccggtg tctggaatat
      541 atttccactg cccatctccg accctccgac cgattgatcg aaccagtgca catcggtgcc
      601 cgtagcgctg atctgcgcgg acccggagcc catgatggtg gcgccggtgc cctgcggttg
      661 tggtgtgttc gccacttcca cggtggccac cggggtggcg tccgtaccgc aattgcccgt
      721 cacggtgcat gaataatcgc ccggggtggt caccgtgatg ctctgggtgg tggcacccgt
      781 gctccaaagg tgcgaggtcc ccgcgctggc cgtgagttgc acgctcccac cttcgcaaaa
      841 ggaggtaggc ccatcggccg agatctgcgc gaaatggcca ccgccgggca cttgctgggt
      901 gagcgtgaag gggacggcgg tccagttgtt gccatcgttc tcctccagga agttgttgtg
      961 aggatccact tccatcacga tccagtagtt gccgttgcac gtgcccgggg gaatgttgat
     1021 ccacatgcca tccaaggatt cgctgtacac atcggtgtaa cccgaggaaa tgccctggct
     1081 gatctggctg cagttatagc tgccgccccc atgcccgtag ttggggaact ggctggtctg
     1141 gttcaacggg ctgccgcctt ggtactcctg tgatgtgcga cagtggccgt tggcgctgct
     1201 gttggcgcag ctgtagtaat ccatcaggca gaagccgacc ttggcacctt caccaacaat
     1261 gggccagttg cgcggatcgg ggtccgccgt ttggatgcgc aaggtgaagg tggcccaatc
     1321 gtccacgtgg ttatgcccgt gggtgggatg gtaggtcatg gtgccggcga accgctcata
     1381 aaaggtcatg gccgccccgt tcttgtggta cacccgttgc aggatcaatt gcttggccgt
     1441 ctgctgctgg ttggggcatt ggaactgctg gctgctgttg gggtcgttga tggagatggt
     1501 atccgtgccg cacaggaacc agcgattccc ctgggcgtcc acaccgcgga cgttgagcgc
     1561 tccatggccg atgttcggcg tggatccggt gacgcgcagc cgccccggat tgctggcatc
     1621 gttctgcgca t
//
LOCUS       DAOQOC010000800         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_57892_length_1630_cov_4.458413, whole genome shotgun sequence.
ACCESSION   DAOQOC010000800 DAOQOC010000000
VERSION     DAOQOC010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57892_length_1630_cov_4.458413"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PL106_11900"
     CDS             <1..657
                     /locus_tag="PL106_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNA33817.1"
                     /translation="VLWGLEQKNLARTRFPVGHYRKTEIREMAAKSGFGRLAQKSESY
                     EICFIPDNDYRGFLKRKMPASFAKLAHGKLVDTDGTVLGEHDGYPFFTIGQRKGLGIA
                     TGTPLYVTDIRPDTNTVVLGGKHDLLKQTAWVREPNFQKIPALIGEMEAITRIRYKDK
                     GTPSTISMANGADGRTGQHVKVEFHAPVGGIAPGQSAVFYEGNDLLGGGFIDREPAHP
                     "
     gene            654..1334
                     /locus_tag="PL106_11905"
     CDS             654..1334
                     /locus_tag="PL106_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33818.1"
                     /translation="MNHRPHFLLLLAAVLTLVACREDEPAEADIGHAYFPVNIGHWVE
                     YHVDSIRQHSDNNAPPDTVVYSFDIREELVEQLTDNEGRPVQRILRTTRDGNGNWIPK
                     DVWWQAREQVRGERTEENMRRVKLYFPPRTGTRWNTNARNTDDAFEIGYTSVDQPYSV
                     NGLNFENTVTVEGTYPPNPFITRNYRERWAKGVGLIEHEVDSINDQHSLGYNRFYVRY
                     TITAYGDQ"
     gene            1334..>1630
                     /locus_tag="PL106_11910"
     CDS             1334..>1630
                     /locus_tag="PL106_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33819.1"
                     /translation="MIPRGILLCILLPVLVTTSAQSPPTTCLIRFNNKSGTPFAPDAP
                     EAFLSPRAIDRRAIQGIPVDSTDLPVDPVYITTVLAQGDVQLVNRSKWFNAITIR"
BASE COUNT          356 a          550 c          468 g          256 t
ORIGIN      
        1 gtgctgtggg gcctggaaca gaagaacctc gcccgcacgc gattccccgt ggggcactac
       61 cgaaaaacag agatacggga aatggccgcc aagagcggct tcgggcgtct ggcccagaag
      121 agcgagagct acgagatctg cttcatcccc gacaacgact accggggctt cctcaaacgg
      181 aagatgcccg cctccttcgc gaagctggcc catggcaagc tcgtggacac ggacggcacc
      241 gtgctgggcg agcacgacgg ttatcccttc ttcaccatcg gccagcgcaa gggcctgggc
      301 atcgccaccg gtaccccact gtacgttacc gatatacgtc cggacaccaa caccgtggtc
      361 ctgggcggca agcacgacct gctgaaacag accgcctggg tgcgcgaacc caacttccag
      421 aagatccccg ccctcatcgg cgaaatggag gcgatcacca ggatccggta caaggacaag
      481 ggcaccccca gcacgatcag tatggccaac ggggccgacg gccggaccgg tcaacacgtg
      541 aaagttgaat tccatgctcc cgtaggaggc atcgcaccgg ggcagagtgc cgtcttttac
      601 gaagggaacg acctgctcgg tggcggtttc atcgacaggg agcccgcgca cccatgaacc
      661 accgccccca cttcctcctg ctgctcgccg cagtgctcac actcgtggca tgccgggagg
      721 acgaacccgc cgaagcggac atcggccatg cctacttccc cgtgaacatc gggcactggg
      781 tggagtacca tgtggacagc atccggcagc actcggacaa caacgccccg cctgacacgg
      841 tggtctacag tttcgatatc cgtgaagagc tggtggaaca gctgaccgac aacgaaggcc
      901 gccccgtgca acgcatcctc cgcaccaccc gcgatggcaa cggcaactgg atccccaagg
      961 acgtgtggtg gcaggcgcgc gagcaggtcc ggggcgaacg taccgaggag aacatgcgcc
     1021 gggtgaaact ctatttcccc ccgcgcacgg gcacccgatg gaacaccaat gcgcgcaata
     1081 ccgacgatgc gttcgagatc ggctacacgt ctgtggatca gccgtacagc gtgaacggcc
     1141 tcaacttcga gaacacggtg acggtcgagg gcacctaccc gcccaatccg ttcatcaccc
     1201 ggaattaccg ggagcgctgg gccaagggcg tcggtttgat cgagcacgag gtggacagca
     1261 tcaatgacca gcactccctc gggtacaacc ggttctatgt ccgctatacg atcaccgcat
     1321 acggcgatca ataatgatac cacgcggcat cctgctgtgc atcctcctcc ctgtgctggt
     1381 gacgaccagt gcacagagcc ctcccaccac ttgtctcatc cgcttcaaca acaagagcgg
     1441 cacgcccttc gctcccgatg ctcccgaagc gttcctgtcg ccccgcgcaa ttgaccgccg
     1501 tgccatccag ggcatcccgg tggactcgac ggacctgccc gtggatccgg tgtacatcac
     1561 cacggtactg gcacaagggg atgtgcaact cgtgaaccgg agcaagtggt tcaacgccat
     1621 caccatccgc
//
LOCUS       DAOQOC010000801         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_58414_length_1623_cov_4.183036, whole genome shotgun sequence.
ACCESSION   DAOQOC010000801 DAOQOC010000000
VERSION     DAOQOC010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58414_length_1623_cov_4.183036"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PL106_11915"
     CDS             complement(<1..393)
                     /locus_tag="PL106_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33820.1"
                     /translation="MRSVIAFIVFFMTDGIACSQNLVPNGGFEELNDCPNNGGQLQKT
                     THWFRGGVSSPDLFNICDGMDTCGVPRNIFGYQFPASGDGYCGMWAFSNASLPGYGTH
                     EIIGVRLSLPLTPGQTYWASFRISWTSSF"
     gene            855..>1623
                     /gene="ribF"
                     /locus_tag="PL106_11920"
     CDS             855..>1623
                     /gene="ribF"
                     /locus_tag="PL106_11920"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNA33821.1"
                     /translation="MQVHTDLSTFVNVERPVLTTGTFDGVHLGHQTILRRLMETARRE
                     GGQSVLFTFHPHPRMVLFPHDNELKLLNTRKEQNELLEAAGLDHLIVVPFSRSFSRMH
                     ALDFVRDTLVDQLRIHAMVIGYDHRFGRNREGDIHLLRQLGEAYDFGVEEIPAHEIDH
                     VKVSSTKVREALLRGEAKLAHDLLGYAYPLGGVVVKGDQRGRTIGFPTANVGGIDPGK
                     LLPADGVYAVEVDLRDGVYKGMLNIGLRPTVEGRDRTV"
BASE COUNT          337 a          468 c          481 g          337 t
ORIGIN      
        1 aaaagatgag gtccaactta tcctgaaaga agcccaatac gtctggcctg gggtcaaggg
       61 caaggacaat cggaccccga tgatctcatg ggtgccatat ccgggcaatg aagcgttcga
      121 aaaggcccac attccacaat atccatctcc actggcgggg aactgatagc cgaagatgtt
      181 gcgcggtaca ccgcaggtgt ccatgccatc gcaaatgttg aacaggtctg ggcttgaaac
      241 accgcctcgg aaccaatgag tggtcttttg caactggcct ccattattcg ggcaatcgtt
      301 caattcttcg aaaccgccat tgggtacgag gttctggctg catgcgatgc cgtctgtcat
      361 gaagaagaca atgaaggcga tgacggatct catggtacgg tgggaggtca gggggggtag
      421 gtgaacggtt ctatcctgag tttcatgggt aggggttgag gccgccgttg gtcaccaggt
      481 tctgcgaccg tgcggttcct cttggtgcta acaggatggc cgaggtcgag atcgcgagat
      541 tctcccactt ccatctgcgc tgcttcatat gaccaaggtt caatgtaccg gttcgggatc
      601 gttccgagca ctcacccgaa aagtatgcct tctactctac gtgattgtgc ttcggcagta
      661 acaaataccg catttgaccg catttgtcgc aagtgttcgc gcccaagacc ttgtccaacg
      721 ctcaggcgcg gccacttgtg gccacacaca cgcctgacaa cgaccgtcgt tgtccaaagc
      781 ttacgccgaa tatcctgtag aagaagcacg tccccgccca tctttggcct ccccgccttg
      841 gcggaatgaa gcacatgcag gtccacaccg atctctccac cttcgtgaac gttgagcgcc
      901 cagtgctcac caccggtacc ttcgatggtg tacacctcgg acaccagacc attctgcggc
      961 ggctgatgga gacggcccgg cgcgagggag gtcagagcgt gctcttcacc ttccatccgc
     1021 acccgcgcat ggtgctcttt ccgcacgaca atgagctcaa gttgttgaac acgcgcaagg
     1081 aacagaatga attgctggag gccgctggtc ttgatcacct gatagtagtg ccattcagcc
     1141 gcagcttcag ccgcatgcac gccttggatt tcgtgcgtga cacgctggtg gaccagttgc
     1201 gcatccacgc catggtcatc ggctacgatc atcgcttcgg ccgcaaccgc gagggcgaca
     1261 tccacctgtt gcggcaactg ggcgaggcgt acgacttcgg ggtggaggag atccccgccc
     1321 acgagatcga tcacgtcaag gtgagcagca ccaaggtgcg cgaggcgctt ttgcgtggtg
     1381 aggcgaaact ggcgcatgac ctgctcggct acgcgtaccc gctcggcggt gtggtggtga
     1441 aaggcgatca gcgcgggcgc accatcggtt ttccaacggc caacgtcggc gggatcgatc
     1501 ctggcaaatt gcttcccgct gatggggtgt acgccgtgga ggtggatctg cgcgacggcg
     1561 tgtacaaggg aatgctcaac atcggcctgc gccccacggt ggagggccgc gaccgcaccg
     1621 tgg
//
LOCUS       DAOQOC010000802         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_58744_length_1619_cov_3.077366, whole genome shotgun sequence.
ACCESSION   DAOQOC010000802 DAOQOC010000000
VERSION     DAOQOC010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58744_length_1619_cov_3.077366"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PL106_11925"
     CDS             complement(<1..261)
                     /locus_tag="PL106_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNA33822.1"
                     /translation="MSRTRTATEAEITLDELKEEILRDYALCVTSREASLIGRKEVLG
                     GKAKFGIFGDGKELAQVCMAKQFRNGDWRSGYYRDMTFMFAIG"
     gene            complement(377..1045)
                     /locus_tag="PL106_11930"
     CDS             complement(377..1045)
                     /locus_tag="PL106_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA33823.1"
                     /translation="MSEYRVKILNTEFITPDVKRLTVEKPKGYVFTPGQATDVAIDAD
                     GLRDQWRPFTFTSLTTSRHLEFIIKIYRDHDGVTKRIGLLHEGDGLILQEPFGTIEYR
                     GPGFFIAGGAGVTPFVAILRDLHHKNAIRGNTLLLTNRMAVDVIMDEEFTKMLGKNFL
                     KIFTRQNVLGFHERRIDRDLLITLVQDFDQHFYVCGPEGFVKDINALLVDLGASAEQL
                     VFER"
     gene            1199..>1619
                     /locus_tag="PL106_11935"
     CDS             1199..>1619
                     /locus_tag="PL106_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA33824.1"
                     /translation="MSQGEPSRLKVYLAFAAIYIIWGSTYYGIKEALTGFPPFMLGGL
                     RFAVAGTLLFAWCAITRTPVFPVGGLWKALLTGFLLLFLGNGAVVWAQQTIPSSVVAI
                     TIAVAPLTFTLFDRPNWGGNFRSPATLSGVLAGVIGVW"
BASE COUNT          265 a          520 c          501 g          333 t
ORIGIN      
        1 gccgatggcg aacatgaagg tcatgtcccg gtagtagccg ctgcgccagt cgccattgcg
       61 gaactgcttg gccatgcaca cctgtgcgag ttccttgccg tcgccgaaga tcccgaactt
      121 ggccttgccc cccagcacct ctttacgccc gatgagggac gcctcgcggc tggtgacgca
      181 caaggcgtag tcccgcagga tctcctcctt cagttcgtcc agcgtgatct cggcttcggt
      241 cgcggttcgg gttcgggaca tcagtcttgg gcttccgcgc gaaaataggc cgctcgtgcc
      301 tttggtgctt cgctgcgacc ggtccctgcg tctttggatc cccggcgaac gggaccacat
      361 cgcgcatggg ccgcgttcag cgctcgaaca cgagctgctc cgcagaggcc cccagatcca
      421 ccaggagcgc gttgatgtct ttcacgaaac cctccggacc gcagacgtag aagtgctgat
      481 cgaaatcctg caccagcgtg atcaacaggt cgcgatcgat ccggcgttca tggaagccga
      541 ggacgttctg ccgcgtgaag atcttcagga agttcttccc gagcatcttg gtgaactcct
      601 cgtccatgat cacatccacc gccatgcggt tggtgagcag cagcgtgttg ccgcggatcg
      661 cgttcttgtg gtggaggtcg cgcaggatgg ccacgaaggg tgttaccccg gccccaccgg
      721 cgatgaagaa gcccgggcca cggtattcaa tggtgccgaa cggttcctgc aggatcaggc
      781 catcgccttc gtgcagcagc ccgatccgct tggtcacacc gtcgtgatca cggtagatct
      841 tgatgatgaa ttcgaggtgg cgcgacgtgg tgagcgaggt gaacgtgaag ggccgccatt
      901 gatcgcgcag accgtccgca tctatggcca catccgtggc ctggccgggg gtgaacacgt
      961 agcccttggg cttctccacc gtcaaccgtt tcacatccgg ggtgatgaac tcggtgttca
     1021 ggatcttgac gcgatactcg ctcatgcgcc aaagatcgct ctaccggcac gttccctgga
     1081 tccgcgttcc ccggacccgc tccggtcggc gcgtccggtc gatcgcggcg accaccgcac
     1141 aaccacttcg gatccccgat gcggaggtcg gtacgaacat tgaacatctt tgcgccccgt
     1201 gagccagggt gaaccctccc gcctgaaggt gtacctggcc ttcgccgcca tttacatcat
     1261 ctggggaagt acgtattacg gcatcaagga ggcgctcacg ggattcccgc cattcatgct
     1321 cggcggcctg cgtttcgccg tcgcgggcac cctcctgttc gcgtggtgcg cgatcacgcg
     1381 cacgccggtg ttccccgtgg gcggcctgtg gaaggccctg ctcacgggct tcctcctgct
     1441 gttcctgggc aacggggccg tggtgtgggc gcaacaaacg ataccgagtt ccgtggtggc
     1501 catcaccatt gccgtggcgc cactcacctt caccctgttc gatcggccga attggggcgg
     1561 gaatttccgc agcccggcca ccttgtcggg tgtgctggcg ggggtgatcg gcgtatggt
//
LOCUS       DAOQOC010000803         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_58754_length_1619_cov_2.585678, whole genome shotgun sequence.
ACCESSION   DAOQOC010000803 DAOQOC010000000
VERSION     DAOQOC010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58754_length_1619_cov_2.585678"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PL106_11940"
     CDS             complement(<1..1521)
                     /locus_tag="PL106_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33825.1"
                     /translation="MQYPSRSLLPLAPFLLPFFLYAQPALIRRLQSNLVPVSLGRLDG
                     QWHLTGYLGENSGWAGVHTLLTEDLAPTGIAKTWPLNAYHEAMSTHKLDNGRWLSFGR
                     SNLPFADRYLYFVEHDAAGTVHAAHKWVDTTSTLTTLNYAHAYNADSSKLLTYFQNLQ
                     VKGFVCFDRNGELLWSRRLMSGNLIMWSLQTASDGGWYGFGRMSFNPDGSDPILVKLD
                     TDGNWLWSHRYRIPYSDETALFARPTTDGGLLLTNTSIIDTLEEDQPYYATLLRTNGD
                     GELLWHRVIGRADGPGRYQGGCAIERPDGSVLWAGSAKDSIADVFINGSMFLLRLDAN
                     GSTISANNYAHTGGGNADNVKFLNSEPPILTARVSDGDSYAMELIVFSDNGLPPCVDA
                     PLTYGSYDWPITDTSFIQYTTPFTLVRDTLILSDPLITITTELWCIATEVPGSTAMDA
                     DIHVNIANGQLVLEAGERPLRQVTVHDATGRLLLRRDQFNGIGRIQLPMPEIVSGLL"
BASE COUNT          297 a          434 c          567 g          321 t
ORIGIN      
        1 tagaagtccg ctgacgattt ccggcattgg cagctggatg cgtccgatcc cattgaactg
       61 gtccctgcgc aggagcagcc gaccggtggc gtcatgcacg gtgacttgtc tcagcggccg
      121 ttctccggct tcaagcacca actgaccgtt ggcgatgttg acgtggatgt ccgcatccat
      181 tgcagtggaa ccggggactt cggtagcgat gcaccagagt tccgtagtga tggtgatgag
      241 cgggtcgctg aggatgagcg tatcgcgcac cagcgtgaag ggcgtggtat actggatgaa
      301 actcgtgtcg gtgatgggcc agtcgtagct gccgtaggtg agcggggcat ccacgcacgg
      361 cggcagacca ttgtcgctga acacgatgag ttccatggcg tagctatcac cgtcagagac
      421 gcgggccgtc aagatgggcg gctcactgtt caggaacttc acattgtcgg cgttgccacc
      481 gcctgtgtgc gcatagttgt tggcgctgat ggtgctcccg ttcgcatcga ggcgcagcag
      541 gaacatgctg ccgttgatga agacatcggc gatgctgtct ttcgctgagc cggcccagag
      601 cacggagcca tcggggcgtt cgatggcaca gccgccctgg tagcggccgg ggccgtcggc
      661 ccggccgatc acgcggtgcc acagaagttc gccatcgccg ttcgtgcgca ggagcgtggc
      721 gtaatagggt tggtcttcct cgagcgtgtc gatgatgctg gtgttcgtga gcagaaggcc
      781 gccgtccgtg gtggggcgtg cgaagagcgc tgtttcgtcc gagtatggga tgcggtagcg
      841 gtgcgaccac aaccagttgc cgtcagtgtc cagcttgaca aggatcgggt ctgagccatc
      901 cgggttgaag ctcatgcgtc cgaacccgta ccagccgccg tcactggcgg tctgcaggga
      961 ccacatgatc agattgccgc tcatgagccg gcgcgaccag agcagttcgc cgttgcggtc
     1021 gaagcagaca aagcccttca cctgcaggtt ctggaagtag gtgagcagct tggagctgtc
     1081 cgcgttgtac gcgtgcgcgt agttgagggt ggtgagcgtg ctggtggtgt ccacccattt
     1141 gtgcgctgca tgcacagtgc cggccgcgtc atgctccacg aaatagaggt agcggtcggc
     1201 gaaggggagg ttgctgcggc cgaaggagag ccaacgaccg ttgtcgagct tgtgcgtgct
     1261 cattgcttcg tggtaggcgt tcagcggcca cgtcttcgcg atgccagtgg gcgcgagatc
     1321 ctccgtgaga agcgtatgaa caccggccca accactgttc tcgcccaggt agccggtcaa
     1381 atgccattgg ccatcgagac ggccgagcga gaccgggacc aaattggact gcaagcgtcg
     1441 gataagcgcg ggttgagcgt acaggaagaa gggcaggaga aaaggtgcca gtggaaggag
     1501 ggaacgcgat gggtactgca ttgaccggat ctgtctgctg aaagacgaag gcgtccgatg
     1561 gtggttgccg ttgcggatct cttccacgag gcggccgacg gtggcggtgg cctcgatca
//
LOCUS       DAOQOC010000804         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_58831_length_1618_cov_2.653231, whole genome shotgun sequence.
ACCESSION   DAOQOC010000804 DAOQOC010000000
VERSION     DAOQOC010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58831_length_1618_cov_2.653231"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..569
                     /locus_tag="PL106_11945"
     CDS             204..569
                     /locus_tag="PL106_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33826.1"
                     /translation="MATTSSCWTSYNPGGIGLDVLRELGSLDKHGNVIIISARDPLDD
                     NLTKPFHLAEFNASTKASGKVRPDPSVGDFKALFELRESAGTAGTCSSRSQPTQKDPG
                     GPGVLLWSKKTSVSVRAEA"
     gene            complement(547..>1618)
                     /locus_tag="PL106_11950"
     CDS             complement(547..>1618)
                     /locus_tag="PL106_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33827.1"
                     /translation="TACDDGNSSTGNDTWTMACACEGQLIDCDGVIGGPALPGLPCDD
                     GDPNTGNDVLDGACDCTGLLLDCEGVPGGANTIGSTCDDGNANTSGDVYNTACICAGI
                     LPNDCEGVAGGTAQPGTACDDGDVNTGNDVYGSDCLCVGQLIDCEGTIGGPALPGTAC
                     DDGVACTVNDVRGTDCACAGTPLTIGAVTGATTVIGNTSNAFVITPIANATSYSWTLP
                     SGWTTSDNGAFVLLAEANNTPGPVELCVTVMVGACELTSCIIVTVDLNTGVATNDATS
                     TDLVTVQPNPSNGLFQVSATNISALNIEVRDGLGRSVLIPMVLNTSHASVLDLSDLAP
                     GAYYLMASSADRQQVVKLLRER"
BASE COUNT          308 a          492 c          509 g          309 t
ORIGIN      
        1 gtggcggagg ggctggactc acaacggcat gcgtcatgcc caattccttc ccgtttctcc
       61 ccgggagttt cgccgaatgc agatcctggt gatggaggat ggatcccagc ttgccgaggt
      121 gatcacgcat tcgttgacgc gggagcaatt caccgtgaag attgccgtca agatgggcga
      181 ggctttgtgc aagacggccg accatggcta cgacctcatc ctgttggaca tcctacaacc
      241 cagggggtat cggcttggac gtgttgcgcg aactgggatc cctggacaaa cacggcaacg
      301 tgatcatcat ctccgccagg gatccgttgg acgacaacct caccaagccc ttccatctcg
      361 ccgagttcaa cgcgagcacc aaggccagcg gcaaggttcg ccccgatcca tcggtgggcg
      421 acttcaaagc gttgttcgag ttgcgcgaaa gcgccgggac agcggggacg tgcagctcaa
      481 gatcgcagcc aacacagaag gaccccggag ggcccggggt ccttctgtgg tcgaagaaga
      541 caagcgtcag cgttcgcgca gaagcttgac cacttgttgc cggtcagctg atgaggccat
      601 caggtagtac gcgcccggtg ccaggtccga gaggtcgagc acggaggcat gtgaagtgtt
      661 cagcaccatc gggatcaata cggaacggcc caagccatcc cgcacctcga tgttgagcgc
      721 cgatatgttg gtcgcgctta cctggaacag cccgttcgat gggttgggtt gcacggttac
      781 cagatcggtg gaagtcgcgt cattcgttgc gacacccgta ttgagatcca cggtcacgat
      841 gatgcagctg gtgagttcgc aggcaccgac catcaccgtc acgcagagtt ccacgggccc
      901 cggtgtgttg ttggcttccg cgaggagcac gaaggcccca ttgtcgcttg tggtccatcc
      961 ggagggaagc gtccaggagt agctggtggc attggcgatg ggcgtgatca cgaaagcatt
     1021 ggaggtattc ccgatcaccg tggtggcacc ggtcaccgcg ccgatggtga ggggtgttcc
     1081 agcgcaggcg caatcagtgc cgcgcacatc gttcaccgtg caggccacgc cgtcatcaca
     1141 agcggtacct ggcaatgcgg gtccaccgat ggtgccttcg caatcgatca gctgaccgac
     1201 gcacaggcaa tcgctcccat atacatcatt gccggtattc acatccccat cgtcgcaggc
     1261 ggtgccgggc tgtgctgttc cgcccgccac accttcgcaa tcgttcggca ggatgcccgc
     1321 gcagatgcac gcggtattgt acacgtctcc ggaggtgttc gcattgccat cgtcgcaggt
     1381 gctgccgatg gtgttcgctc cgccgggaac gccttcgcag tcgagcagca ggcccgtgca
     1441 gtcgcaagcg ccgtcgagca catcattgcc cgtgttcgga tcgccatcat cacagggcag
     1501 gccgggcagc gcggggccgc cgatcacgcc gtcgcaatcg atcagctggc cttcgcacgc
     1561 gcaagccatg gtccaggtgt cgttgccggt gctcgagttg ccatcatcgc aggccgtg
//
LOCUS       DAOQOC010000805         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_58977_length_1616_cov_3.016656, whole genome shotgun sequence.
ACCESSION   DAOQOC010000805 DAOQOC010000000
VERSION     DAOQOC010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58977_length_1616_cov_3.016656"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PL106_11955"
     CDS             <1..762
                     /locus_tag="PL106_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNA33828.1"
                     /translation="GSILSRFADAHIVDISHQITPFDNMHAAFTLRNAWAEFPVGTIH
                     IIGVNSEADGHTPHLVVRHMGHYFIGADNGIFSLLFDGKPNEAFELTIMSDPGHTAFP
                     IKSVFVKAACHLARGGAPETIGRKVTSTREQLSFRPSVLADAIKGVVIHIDSYGNVVT
                     NITRAHFNELVKAHTFRITFGRTAHDITTISNSYGDVPSGERVAFFGATGFLEIAVNK
                     GVTGGGGGAAQLLGLRVADPVRVELTGMLRNAANG"
     gene            759..1064
                     /locus_tag="PL106_11960"
     CDS             759..1064
                     /locus_tag="PL106_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNA33829.1"
                     /translation="MIVRLVKMTFRPDEVEAFQELFEGWRHRIIAFPGCRRLELLHGT
                     DDPRVFFTYSEWDGPEALDAYRASDVFADVWPVVKGLFAARAEAWTTVREHAMDSVR"
     gene            1124..>1616
                     /locus_tag="PL106_11965"
     CDS             1124..>1616
                     /locus_tag="PL106_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA33830.1"
                     /translation="MRTSLLPFVAATSLSVAVRAQHPYDVALAAANAGLLDSAMASVQ
                     RAIVHDPADARAYKLRGDLHQRKDEVDEALDDYNRSEQLDPSNARLYISRSALRISKG
                     MLKTALKDCEMAIELAPDDPDGYYNRACLLYLGDNVEGARKDAERAVKLDPQHADALY
                     LAGV"
BASE COUNT          298 a          522 c          506 g          290 t
ORIGIN      
        1 ggttcgatcc tgagccggtt cgccgatgcg cacatcgtgg acatcagcca tcagatcacg
       61 cccttcgaca acatgcacgc ggccttcacc ctgcgtaatg cctgggccga gttccccgtg
      121 ggcaccattc acatcatcgg ggtgaacagc gaggccgatg gacatacgcc gcacctcgtg
      181 gtgcgccaca tgggccacta tttcatcggt gcggacaacg ggatcttctc cctgctcttc
      241 gatggcaagc ccaacgaggc cttcgagttg accatcatga gcgacccggg ccatacggcc
      301 ttccccatca agagcgtgtt cgtgaaggcc gcctgccacc tggcgcgcgg aggcgcaccg
      361 gagaccatcg gccgcaaggt caccagcacg cgcgagcaac tcagcttccg cccttcggtg
      421 ctcgccgatg cgatcaaggg agtggtgatc cacatcgatt catacggaaa tgtggtgacc
      481 aatatcaccc gcgcccactt caacgaactg gtgaaagcgc acaccttccg gatcacgttc
      541 ggtcgcaccg cgcacgacat caccaccatc agcaactcgt acggcgatgt gccctccggt
      601 gagcgcgtgg cctttttcgg tgccacgggc ttcctggaga tcgctgtgaa caagggcgtg
      661 accggcggag gtggcggcgc ggcccagctc ctgggactgc gcgtggccga tccggtgcgg
      721 gtggaactta ccggcatgct ccgcaacgcg gccaacggat gatcgtccgc ttggtgaaga
      781 tgaccttccg cccggacgag gtggaggctt tccaagagtt gttcgagggc tggcgtcacc
      841 gcatcatcgc gttcccgggt tgccgccgct tggaactgct gcatggtacg gatgatccgc
      901 gtgtcttctt cacatacagc gaatgggacg gccccgaagc actggatgcc tatagggcat
      961 ccgacgtttt cgccgacgtt tggcctgtgg tgaaaggact tttcgccgcg cgtgccgaag
     1021 cctggaccac cgtgcgcgag cacgccatgg acagcgttcg ctgaggggga tcatctttgt
     1081 gcgtccgctc ggaggcccct ccgtgcttcg acgtgacttg cgaatgagaa catccctcct
     1141 gcccttcgtc gccgcaacgt cgctgagtgt ggccgtgcgt gcccagcatc cgtacgatgt
     1201 cgccctggcc gcggccaacg ccgggctgct ggacagtgcc atggccagtg tgcagcgtgc
     1261 cattgtccat gaccccgccg atgcccgcgc gtacaaattg agaggcgatc tgcaccagcg
     1321 gaaggacgag gtggatgagg cgctggacga ctacaacagg agcgagcaac tggaccccag
     1381 caacgcgcgc ctgtacatca gccgctcggc cctgcgcatc agcaagggca tgctgaagac
     1441 cgcgctgaag gattgcgaaa tggcgatcga actggcgccg gacgaccccg atggctatta
     1501 caaccgcgcc tgcctgctgt acctgggcga caatgtggag ggcgcgcgca aggacgccga
     1561 acgtgccgtg aaactggatc cccagcacgc ggacgcgctc tacctggcgg gtgttg
//
LOCUS       DAOQOC010000806         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_58994_length_1616_cov_2.647021, whole genome shotgun sequence.
ACCESSION   DAOQOC010000806 DAOQOC010000000
VERSION     DAOQOC010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58994_length_1616_cov_2.647021"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1488
                     /locus_tag="PL106_11970"
     CDS             100..1488
                     /locus_tag="PL106_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNA33831.1"
                     /translation="MHFPRSIKRDLSDLVSPIGFATGLTALFALTANAQTGVAIGTGS
                     PTAHASAMLDVQSTNKGMLVPRMTSAQRTAIASPAAGLLVFDTTLGQFHYYTGGSWQA
                     IAPGSGFAWSVTGNAGINPDTQFIGTTDDQPIIMKQNGQRIASLKWDNIAFGTNSLQA
                     TTTGTFNFAMGNDVGTALTTGNHNVMLGDQAMREADPDAGFNTVVGCNAGKLITGNWN
                     TVMGSEAGHYAGSKNVAVGTLAGYSGDAGNTCLGYDAGPDVAGASNCVFVGNGSTSST
                     LDLTNSIAIGYNRTVIANNQVRIGNTSMTSIGGQVGWSTLSDARTKKNVNANVHGLDF
                     ILKLRPVTYNYDMAATIALNNEGQRTDPKTGKTEAIEPTANDQQARDAQGRVTYTGFI
                     AQEVEQAAKEVGYDFSGVDAPKNADDLYSLRYAEFTVPLVKAVQEQQAMIDAQQQLIA
                     DLQRRLQQLEAR"
BASE COUNT          384 a          497 c          462 g          273 t
ORIGIN      
        1 attgtagcga ccagttgtcg ggccataccg ccagatatcg gaagtggctg tgtacagcgc
       61 atcgggccag ggacttttgg cccaccaaac acacaacaca tgcacttccc ccgttcgatc
      121 aagcgcgacc tgtcggacct cgtgtccccg atcggcttcg ccaccggcct cacagcgctc
      181 ttcgccctta cggccaatgc ccagaccgga gtagccatag gaacaggcag ccccaccgca
      241 catgccagcg ccatgctcga tgtgcaaagc accaacaaag gcatgctggt gccgcgcatg
      301 accagtgcac aacgcacggc gatcgcttca cctgcggcag gattgctcgt cttcgacacc
      361 acgcttggac aattccacta ttacaccggc ggttcctggc aggccatcgc gccgggcagc
      421 ggatttgcct ggagcgtcac gggaaacgct ggtatcaacc ccgacacaca gttcataggt
      481 accacggacg accagccgat catcatgaag cagaatggtc aacggatcgc gagcttgaag
      541 tgggacaaca tcgcctttgg caccaactcg cttcaggcga ccacgaccgg tacgttcaac
      601 tttgcgatgg gcaacgatgt cggcaccgct ctgaccactg ggaaccacaa tgtgatgctg
      661 ggcgatcagg ccatgaggga agcggatccc gatgccggtt tcaatacggt cgtgggttgt
      721 aatgccggca agttgatcac tggaaattgg aatacagtaa tgggttcgga ggccgggcac
      781 tatgcgggct ccaaaaacgt agctgtgggg accctggcgg gttattccgg tgacgctggg
      841 aatacatgtt taggctatga tgccggtccg gatgtcgctg gagcttcgaa ctgtgtcttc
      901 gtgggaaatg gttcaacgag ttccacgttg gatctgacca actccatcgc catcggctac
      961 aaccgcacgg tcatcgccaa taaccaagtg cgcatcggca acacgtccat gaccagcatc
     1021 ggtgggcagg tgggctggag cacactgagc gatgcacgca ccaagaagaa cgtgaacgcg
     1081 aacgtgcatg gcctggactt catcctgaag ctgcgaccgg tgacctacaa ctacgacatg
     1141 gcggccacca tcgcattgaa caacgaaggc cagcgcacgg acccgaagac cggcaagacg
     1201 gaggcgatcg aaccgaccgc gaacgatcaa caggcacgcg atgcgcaagg ccgcgtcacc
     1261 tacaccggat tcatcgcaca ggaggtggag caggccgcga aggaagtggg ctacgatttc
     1321 agtggtgtgg acgcaccgaa gaacgcggac gacctctact ccttgcgcta tgcggaattc
     1381 acggtaccgc tggtgaaggc cgtgcaggag caacaggcca tgatcgacgc acagcaacaa
     1441 ctgatcgccg acctgcagcg ccgcctgcaa caactcgaag cacgatgaag aacgcacttc
     1501 tccttgcggc cgtgctgctc accgcgaccg gtcactcaca ggacatcaac ctcggcaccg
     1561 catccatccg catcaacggg ggcaccaacc tcaagacggc agggatcatc accatg
//
LOCUS       DAOQOC010000807         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59002_length_1616_cov_2.408712, whole genome shotgun sequence.
ACCESSION   DAOQOC010000807 DAOQOC010000000
VERSION     DAOQOC010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59002_length_1616_cov_2.408712"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1616)
                     /locus_tag="PL106_11975"
     CDS             complement(<1..>1616)
                     /locus_tag="PL106_11975"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA33832.1"
                     /translation="SFHVSAPLGPLLVTVPADGIICPADSALIFTTTPGTGQVWYGPF
                     GPVPSGSPQLWSGVPGEYYLSMTDTFGCSVISDPALLTGYSTPYLNLIPDNVLCLGEE
                     SAIIQVMTTAAGTIVWAPPLSGSAQTQTVTQPGTYSVSSTACGITTQLSVTVVASSVS
                     ADVLEVGPISLCDGDSITLHAAPGQAVYIWQPGNILADSITVSTAGTYHLQVLDGNGC
                     ADTSDAVIVDLHAISETLVAIGDTICEGETAIITASGSGAMIWFADADTQQVLFSGSS
                     ITLDGIVQDTVFYVQQTDGPCVSGVQAVPVHVDPSPPPVDIDAPGTVCGDGQVIIGLI
                     APPNVSAQWATPGGAFTGTSVTIDDFTPDDAGMYTAIPIIGGCAGVPVSAFIAFTAPV
                     PIDLGPDTTYCIGGYTVLNIPAWYTDPQWSTGATGFTVQVGSDGLITVQAVDSNGCLV
                     LDEVLLSGVDCEPRIPNVISPNGDGSNDGVTITGSSGFALHFRLWNRWGQPVWEATGE
                     DIHWNGRHTNGEALPDGVYYYELLRTGREGP"
BASE COUNT          298 a          482 c          542 g          294 t
ORIGIN      
        1 ggcccctccc tgcccgtgcg caaaagctcg taataataaa ctccatcggg tagcgcttcg
       61 ccgttggtgt gccggccgtt ccaatggatg tcctctcctg tcgcttccca cacgggctgt
      121 ccccagcggt tccacaagcg gaagtgtagc gcgaagccgc tggagccggt gatggtcaca
      181 ccgtcgttgc tgccatcgcc gttgggtgag atcacgttgg gaatgcgtgg ttcgcaatcc
      241 acgccactga ggagcacctc gtccagcaca aggcagccat tgctgtccac ggcctgaacg
      301 gtgatgagcc catcgcttcc cacctgcacc gtgaaacccg tggcgcccgt ggaccattgc
      361 gggtccgtgt accaagcggg gatattgagc accgtgtaac cgccgatgca gtaagtggtg
      421 tcgggcccca gatcgatggg tactggcgcg gtgaaggcga tgaaggcgga cacgggtaca
      481 ccggcgcagc caccgataat gggtattgcg gtgtacatgc ccgcgtcatc gggcgtgaag
      541 tcgtcgatcg tgacggaagt acctgtgaag gcacctcccg gcgtggccca ctgcgcactg
      601 acattcgggg gtgcaataag cccgatgatc acctgtccat cgccacacac cgtgccgggt
      661 gcatcgatat cgacgggcgg cggtgatgga tccacgtgta cgggtacggc ctgaacaccg
      721 ctgacgcacg ggccatcggt ctgctgcaca tagaaaacgg tgtcctgcac aatgccgtcg
      781 agcgtaatgg acgagccgga gaaaaggacc tgttgggtat cggcatcggc gaaccagatc
      841 atggcaccgc tgccgctggc ggtgatgatg gccgtctccc cttcacagat ggtatcgccg
      901 atggcaacga gcgtttcact gatggcgtga agatccacga tgaccgcatc gctcgtgtcg
      961 gcacagccat tgccatcaag cacctgcaaa tggtaggttc cggccgtgct cacggtgatg
     1021 ctgtcggcga ggatgttacc gggttgccag atgtaaacgg cctgcccggg cgcggcgtgc
     1081 aacgtgatgc tatcgccatc gcacaggctg atgggaccga cctccagcac atcggcactc
     1141 acgctgctgg ccaccacggt cacgctcaac tgggtggtga taccgcaggc ggtgctgctc
     1201 acgctgtagg tgccgggctg cgtcacggtc tgcgtctgtg cacttccgct gaggggcggc
     1261 gcccacacga tggtgccggc ggcggtggtc atcacctgga tgatggcgct ctcctcgccg
     1321 aggcagagca cgttgtcggg gatcaagttg aggtagggcg tgctgtaccc ggtgagcagt
     1381 gccgggtcgc tgatgacgct gcaaccgaaa gtgtccgtca tgctcaggta gtattcgccg
     1441 ggcacgccgc tccagagttg gggactgccg ctgggcaccg gcccaaaggg gccgtaccac
     1501 acctggcctg tgccgggcgt ggtggtgaag atgagggcgc tgtccgcggg acagatgatg
     1561 ccgtcggcgg gcaccgtgac aagcagcgga ccgaggggcg cggaaacatg gaagga
//
LOCUS       DAOQOC010000808         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59028_length_1615_cov_4.758974, whole genome shotgun sequence.
ACCESSION   DAOQOC010000808 DAOQOC010000000
VERSION     DAOQOC010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59028_length_1615_cov_4.758974"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1615
                     /gene="scpA"
                     /locus_tag="PL106_11980"
     CDS             <1..>1615
                     /gene="scpA"
                     /locus_tag="PL106_11980"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNA33833.1"
                     /translation="SNAFYRRNLAAGQMGLSVAFDLATHRGYDADHPRVKGDVGKAGV
                     SISSVEDMNLLFDQIPLDKMSVSMTMNGAVLPIMAFFIVAAEEQGVSAEQLQGTIQND
                     ILKEFMVRNTYIYPPGPSMRIVGDIFSYCAKKMPKFNSISISGYHMHEAGAPADLELA
                     YTLADGIEYVRTGMAAGMQVDEFAGRLSFFWGIGMDLFMEVAKMRAGRLLWAKLLNEI
                     GAKDKKSLALRTHCQTSGWSLTAQDPYNNIARTTVEALAAALGGTQSLHTNSLDEAIA
                     LPTDFSAKIARDTQLYLQRNSGITKWIDPLGGSYYVESLTHELVHKAWERIMEVEELG
                     GMAKAIESGLPKMRIEEAAAKRQARIDTGKDKIIGVNAYEVDEETPIELLEVDNAKVR
                     EQQVERLRRVKAARHEGKVKEALEALTSAARSAGLSDVLSPMPNAQGIGHKTSDISDG
                     PARGNLLELAVNAARARATLGEISDALEQAFGRYAAVPRTISGIYSAEAMQDPEMKEA
                     MRLADEFAKQEGRRPRILVAKMGQDGHDRG"
BASE COUNT          364 a          482 c          524 g          245 t
ORIGIN      
        1 gagcaacgcc ttctaccgcc gcaacctcgc ggccgggcag atgggcctca gtgtggcctt
       61 cgacctggcc acccaccgcg ggtatgacgc ggaccatccg cgcgtgaagg gcgacgtagg
      121 caaggcgggc gtcagcatca gcagcgtgga ggacatgaac ctcctgttcg accagatccc
      181 gctggacaag atgagcgtga gcatgaccat gaacggcgcc gtgctgccca tcatggcctt
      241 cttcatcgtg gcggcggaag aacaaggtgt aagcgcggaa cagttgcagg gcaccatcca
      301 gaacgacatc ctcaaggagt tcatggtgcg caacacctac atctatccgc cgggcccgag
      361 catgcgcatc gtgggcgaca tcttcagcta ctgcgcgaag aagatgccca agttcaacag
      421 catcagcatc agtggctacc acatgcacga ggccggtgcg cccgccgacc tggagctggc
      481 ctacacgctg gccgatggca tcgagtacgt gcgcacgggc atggccgccg gcatgcaggt
      541 ggatgaattc gccgggcggc tcagcttctt ctggggcatc ggcatggacc tcttcatgga
      601 ggtggccaaa atgcgcgccg ggcgcctgct gtgggccaaa ttgctcaacg agatcggtgc
      661 gaaggacaaa aagagcctgg ccctgcgcac ccactgccag accagcggct ggagcctcac
      721 cgcgcaggat ccctacaaca acatcgcgcg caccaccgtg gaagcgctgg ccgcagccct
      781 gggcggcacg caatcgctgc acaccaactc gctggacgag gccatcgcgt tgcccacgga
      841 cttcagcgcc aagatcgcgc gcgacacaca gctgtacttg cagaggaaca gcggcatcac
      901 caagtggatc gacccgctgg gcggtagtta ttatgtggaa agcctcacgc acgaactcgt
      961 acacaaggcc tgggagcgca tcatggaagt ggaagaactc ggcggcatgg ccaaggcgat
     1021 cgaaagcggc ctgcccaaga tgcgcatcga ggaagccgct gcgaagcgac aagcccgcat
     1081 cgataccggc aaggacaaga tcatcggtgt gaacgcctac gaggtggatg aggagacccc
     1141 gatcgagttg ctggaagtgg acaatgcgaa ggtgcgcgaa cagcaggtgg aacggttgag
     1201 gagggtgaag gcggcgcggc acgaggggaa agtgaaagaa gcgctggaag cattgacgag
     1261 tgctgcgcgg agcgcagggc tgtccgatgt cttaagtcct atgccaaatg ctcagggcat
     1321 tggacataag acatcggaca tcagcgatgg acctgcgcgc gggaacctcc tcgaactcgc
     1381 cgtcaacgcc gcccgtgccc gtgcaacctt gggggagatc agcgacgcct tggaacaagc
     1441 ttttggccgc tatgctgccg tgccacgtac gatcagtggt atttattctg cggaagccat
     1501 gcaagatccg gagatgaagg aggccatgcg ccttgctgat gaattcgcca agcaggaagg
     1561 acgccgcccg cgcatcctgg tggccaagat gggccaggac ggccacgacc gcggc
//
LOCUS       DAOQOC010000809         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59041_length_1615_cov_3.595513, whole genome shotgun sequence.
ACCESSION   DAOQOC010000809 DAOQOC010000000
VERSION     DAOQOC010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59041_length_1615_cov_3.595513"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PL106_11985"
     CDS             <1..406
                     /locus_tag="PL106_11985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA33834.1"
                     /translation="DAMTECAITFGDLDPMGIVWHGNYFRFMEAGREAFGRMHGLDAM
                     RIHTMGYFTPIVRSVIEHKAPLAYGDSVTVRTWMEPSPAAKVILHYEMRQKAGGHVVA
                     TGSTVQVFLDLDRKLVLDLPEFYRNWKAANGI"
     gene            417..1517
                     /locus_tag="PL106_11990"
     CDS             417..1517
                     /locus_tag="PL106_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNA33835.1"
                     /translation="MNIQLGAAHCLSALGEGVAASFTRMRTGGTAITREEDPNGTLSH
                     VGRIPDEPEDQDRLYRIMDRCLNASMARLMRTPDPQRTQWILASTKGDIGALDRGHPE
                     GAVLPMLAAHVKKRWNTADEPWVVSNACASGTTAIALGGQLVRSGRADHVVVIGIDIL
                     ARFVLEGFRSLHALSAGPCRPFDASRDGTSLGEACATVVLTNDQDLFDAPLGSLLGSG
                     SAHDANHISGPSRTAEGLLRAVQQAMRTSGTTAGDISVINAHATGTPYNDAMEAIAFD
                     RCGLGQVPMSGYKGWFGHTLGAAGVLETVLATHALEQGLALRNEGHTVVDPTLRANVL
                     DRDTPVGGNILLKTSSGFGGCNAALIIRAWKN"
BASE COUNT          307 a          498 c          542 g          268 t
ORIGIN      
        1 ggacgcgatg acggaatgcg ccatcacctt cggcgacctg gacccgatgg gcatcgtgtg
       61 gcatggcaat tacttccggt tcatggaagc ggggcgcgag gcgttcggac ggatgcatgg
      121 gctcgatgcg atgaggatcc acaccatggg atatttcacg ccgatcgtac gttctgtgat
      181 cgagcacaag gccccgctcg cgtacggcga cagcgttacg gtgcgcacct ggatggagcc
      241 ctctcccgcc gccaaggtga tcctgcacta cgagatgcgc cagaaggccg gaggccatgt
      301 ggtggccacg ggcagcacgg tgcaggtgtt cctggacctg gaccgcaagc tggtgctgga
      361 cctgccggaa ttctaccgaa actggaaagc ggcgaacggt atctgagacg gtggtcatga
      421 acatccagct cggcgccgca cactgccttt ccgccctggg tgagggagtg gccgcatcat
      481 tcacccggat gcgaacggga ggcacggcca tcacgcgtga ggaggatccg aacgggaccc
      541 tgtcccatgt gggccgcata ccggatgaac ccgaggatca ggatcgtttg taccgcatca
      601 tggatcgatg cctgaatgcg agtatggccc ggctgatgcg aaccccggac ccgcagcgca
      661 cgcagtggat cctggcaagc acgaaaggcg acatcggcgc cttggaccgg ggccaccccg
      721 aaggagcggt attgcccatg ctggccgccc acgtgaaaaa acgctggaac acggctgacg
      781 aaccctgggt ggtctcgaac gcctgcgcat cgggcacaac ggccatcgcc ctcggcggtc
      841 aacttgtccg ttcgggtcgc gccgatcatg tggtggtcat cggcatcgac attcttgcac
      901 gcttcgtgct ggaaggcttc cgatcgctcc atgcgctgag tgcgggaccg tgcaggccat
      961 ttgatgcctc gcgcgacggc accagcctgg gcgaggcttg cgccaccgtg gtgctcacga
     1021 atgaccagga tctgtttgat gcaccactgg gctcactgct gggcagcggt agtgcgcatg
     1081 atgccaatca catttccggt ccatcgcgca ccgccgaggg gctgctgcgc gccgtgcaac
     1141 aggccatgcg taccagcgga acgaccgctg gggacatcag cgtgatcaac gcgcacgcca
     1201 cgggcacacc gtacaacgac gcgatggagg ccatcgcctt cgaccggtgt ggactcgggc
     1261 aggtaccgat gagcgggtac aagggctggt tcggccatac gttgggtgcg gcgggcgtgc
     1321 tggagacggt gctcgccacc cacgcactgg aacaagggct ggccctgcgc aacgaggggc
     1381 acacggtggt ggatccgacc ttgcgggcga acgtgctgga ccgcgacacg cccgtcggcg
     1441 ggaacatcct gctgaaaaca tcctccggct tcggtggctg caacgccgcc ctcatcatcc
     1501 gcgcatggaa gaactgagca tcctgcgcac cgcacgttat cgcaacggcc gcttcgaggt
     1561 ggaaggaaca gcgtgcaccc cgggtacggg ggatgtggac gctgatctgg caacg
//
LOCUS       DAOQOC010000810         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59354_length_1611_cov_3.080334, whole genome shotgun sequence.
ACCESSION   DAOQOC010000810 DAOQOC010000000
VERSION     DAOQOC010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59354_length_1611_cov_3.080334"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PL106_11995"
     CDS             complement(<1..544)
                     /locus_tag="PL106_11995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA33836.1"
                     /translation="MRTEGREGLDVKAAGDGWVSRIKVGPWGYGKALYIDHPNGHTTV
                     YAHLSEFTDAIADTLLARQYRARSFEVDLEIGRGVLPVKQNQVVAYSGNTGGSGGPHL
                     HFEVRRTSDQHALDPERYGMDVPDDVAPIFSGLRIDALDSSARVQPYPSGAVGFVVAA
                     TGSGYALKDGTVPEAIGTVGF"
     gene            543..788
                     /locus_tag="PL106_12000"
     CDS             543..788
                     /locus_tag="PL106_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33837.1"
                     /translation="MSNPLWKWSLRNSMKLPERERGMLSGEANCGSLGGDCPIEDLID
                     KKLAIKKQHVHFKTVDPIFDAAIGARQEGMLQTKGRW"
     gene            complement(1288..>1611)
                     /gene="paaJ"
                     /locus_tag="PL106_12005"
     CDS             complement(1288..>1611)
                     /gene="paaJ"
                     /locus_tag="PL106_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNA33838.1"
                     /translation="MDVMAADIKQVLREAGITGVEVKQVLSPAWTTDWITEEGRNKLK
                     AYGIAPPPHTADIRALKGCAPVVECPRCGSGNTAMLSAFGSTACKALWKCNDCLEPFD
                     QFKCL"
BASE COUNT          326 a          473 c          485 g          327 t
ORIGIN      
        1 agaatcccac tgtgccgatc gcttcgggca ctgtgccgtc cttcagggca taaccgctgc
       61 cggtggcggc caccacgaag cccactgctc cgctgggata gggctgcacc cgtgccgagc
      121 tgtccaacgc gtcgatgcgc agtccgctga agatcggggc cacatcatct gggacgtcca
      181 tcccgtagcg ctcaggatcc agggcatgct gatcactggt gcgccgcacc tcgaagtgaa
      241 gatgcggacc accgctgcct ccggtgttac cactgtacgc caccacctgg ttctgtttca
      301 cgggtagcac acctctgccg atctccaggt ccacttcgaa ggagcgcgcc cggtactggc
      361 gggccagcag ggtatcggca atggcgtcgg tgaactcgct caggtgagcg tacacggtgg
      421 tatggccgtt gggatggtcg atgtagaggg ccttgccata gccccaaggg cccactttga
      481 tccggctgac ccaaccgtct ccggcggcct tcacgtccag gccttcccgg ccttcggtgc
      541 gcatgtccaa tccgctgtgg aaatggtcgc tgcgcaattc catgaagttg cccgaaaggg
      601 agagggggat gctcagcgga gaagcaaact gcggttcctt ggggggtgat tgtccaattg
      661 aagatctgat tgataaaaag ctggctatca agaaacaaca tgtgcatttc aaaaccgttg
      721 atcccatatt cgatgccgca ataggagcga ggcaggaagg catgctgcaa accaaaggcc
      781 gatggtagcc ccgggcgggc tcgcgcacgg ggtatgcgtt aacagggagg ggttgaagaa
      841 ggggggcgga aacatgcttg cgtggctagc caagcaaatg gagtgcttat ttagaaggat
      901 gcttggctgg ccacgcaagc atgttgtgct caaggaaccc tgactgatcc gcttcaaagg
      961 cacaccgaaa ttcacggaaa ggcgtgtcct tggcggtttg taggagaatt gttgcatcca
     1021 ctggatgctt ggttggccag ccatgcaaat ggagtgctta tttagaatca tgcttggctg
     1081 gccacgcaag gatttcctct gcatccacca tgccttcgtg cccataacga ggccccactc
     1141 ggcgaactgc acgttgatct ccgggctcga cctcctcccg gaggactcct gaatactcta
     1201 cgcatcgcag cggatcaccc agaggcgcgc tgaacaagcg gtgttgaacg gagatgatcg
     1261 gaccagtagg ccgagccatt cacccggtca cagacatttg aactggtcga agggctccaa
     1321 gcagtcgttg catttccata gggccttgca cgcagtgctg ccgaaggcgg aaagcatggc
     1381 cgtgtttccg gagccgcaac gcgggcattc caccacggga gcgcagcctt tcaacgcacg
     1441 gatgtcggcc gtatggggcg gcggcgctat cccgtaggcc tttagtttgt tgcgaccttc
     1501 ctcggtgatc cagtccgtgg tccacgccgg gctcagcacc tgtttcacct ccactccggt
     1561 gatccctgcc tcacgcagga cctgcttgat gtcggccgcc atcacatcca t
//
LOCUS       DAOQOC010000811         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59641_length_1607_cov_3.722938, whole genome shotgun sequence.
ACCESSION   DAOQOC010000811 DAOQOC010000000
VERSION     DAOQOC010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59641_length_1607_cov_3.722938"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PL106_12010"
     CDS             complement(<1..1197)
                     /locus_tag="PL106_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33839.1"
                     /translation="MSTSYRLLLLTGTLVCAHGLLAQLSFGGSALGMDPRGCPLPAAP
                     VIDMPAVDVPALLAEDAHRAATGIKGPWRFGLEESTDISSTGAGVWITHGDLRIWRVQ
                     VHCPEALGIGIIFSTFRVPEGGHVFLYNANGQVLGGFTAASNPGHTVLGVQPMQGSTI
                     TIEYQEPVRVEGQGLLTVGTVIHVYRGTPWAPQSKDDRDFGSSGPCNVNTICPEGDLW
                     RPEIRSVARIFAGGGFCTGTLLNNCANDSIPYFLTANHCLGGGSPDTWVFLFNWDSPV
                     CDPTENGPMDQTVSGSTQLAVNPGSDMLFLRLNSQPPSEFNVTYSGWDATGTPPDSSV
                     CIHHPAGDIKKITHDNNTATQANIDVGNGPADCWNTANYESGTTEPGSSGSALWNQDH
                     RIIGQLY"
     gene            complement(1332..>1607)
                     /locus_tag="PL106_12015"
     CDS             complement(1332..>1607)
                     /locus_tag="PL106_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33840.1"
                     /translation="SVDGTTGSIELPFDRYYLEEGDGPKTEKLLAPDWTSGTPSAPLP
                     SHAVVRILDGEAVITDLVVGDRSIHEWLKDPPPEPTDPASDPVPEEP"
BASE COUNT          289 a          481 c          522 g          315 t
ORIGIN      
        1 gtacaactgc ccgatgatgc ggtggtcctg gttccagagg gcgctcccgc tgctgccggg
       61 ttcggtggtc ccactctcgt agttggcggt gttccagcaa tcggccgggc cgttgcccac
      121 atcgatgttg gcctgggtgg cggtgttgtt gtcgtgcgtg atcttcttga tgtcccctgc
      181 cggatggtgg atgcagaccg aactgtcagg aggggtgccg gtggcgtccc agccgctgta
      241 ggtcacattg aattccgaag gcggttggct gttcagacgc aggaagagca tgtcgctgcc
      301 tgggttcacc gccagttggg tggatccgct cacggtctgg tccatgggtc cgttctccgt
      361 ggggtcgcaa acggggctgt cccagttgaa caggaatacc cacgtgtcgg gcgatccccc
      421 ccccaggcag tggttggccg taaggaagta cgggatgctg tcgttcgcgc agttgttcag
      481 caaggttccg gtacagaatc cgccaccagc gaagatgcgg gccaccgagc ggatctcggg
      541 tctccaaaga tccccttccg ggcagatcgt gttcacgttg caagggccac tgctgccgaa
      601 gtcgcgatca tccttgctct gcggggccca aggcgtaccg cgatacacat ggatcacggt
      661 gcccacggtc agtaggccct gcccttcaac acgaacgggc tcctggtact cgatcgtgat
      721 cgtactccct tgcattggtt gcacgcccag aacggtatgg ccggggttgc tggcagcggt
      781 gaagcccccc agcacctggc cgttcgcgtt gtacaggaaa acgtgcccgc cttccggaac
      841 gcggaacgtg gagaaaatga tgccgatgcc caaggcctcc gggcaatgca cctgtacacg
      901 ccagatccga aggtcgccgt gcgtgatcca cacacccgca ccggtcgatg agatgtcagt
      961 ggattcttcc agcccgaagc gccaaggccc tttgataccg gtggctgcgc gatgtgcatc
     1021 ctcggccagc agagcaggta catccacggc aggcatgtcg atcacgggag cggccggtaa
     1081 cgggcagcca cgcggatcca tgccgagcgc actacccccg aatgagagct gggccaacag
     1141 cccgtgggca cacacgagag ttcctgttaa gagcaggaga cgatacgaag tggacatggt
     1201 tggttcggtc tggtgaacag acgcaacaca cggaagccgc gttgccgggt tgaaagtagg
     1261 gggagttggc gaccggtatc gggaggggcc cggacccgac ctgctccttt tatgtggata
     1321 tccttgctgg ctcacggctc ctccggcact ggatcggagg ccgggtcggt gggctcaggt
     1381 ggtggatcct tcagccattc gtgtatggag cggtcgccca ccaccaggtc ggtgatcacc
     1441 gcttcaccat cgaggatacg caccaccgca tggctgggca agggtgcgga gggcgtgcca
     1501 ctggtccagt cgggcgccaa gagcttctcg gtcttcggcc catcgccctc ctccaagtag
     1561 tacctgtcga atggaagctc gatcgaacct gtcgttccat caacact
//
LOCUS       DAOQOC010000812         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59774_length_1605_cov_4.317419, whole genome shotgun sequence.
ACCESSION   DAOQOC010000812 DAOQOC010000000
VERSION     DAOQOC010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59774_length_1605_cov_4.317419"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PL106_12020"
     CDS             complement(<1..226)
                     /locus_tag="PL106_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA33841.1"
                     /translation="MAALAARFVNSTNRHVFLTGKAGTGKTTFLHKLAASTHKRYVIL
                     APTGIAALNAKGVTIHSQFLLPFGSFLPEKQ"
     gene            533..1312
                     /locus_tag="PL106_12025"
     CDS             533..1312
                     /locus_tag="PL106_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33842.1"
                     /translation="MKHRIALLLCIGIGLSVRAQLTAGEVPDGQIAYDPGIDLELSMP
                     NSVDSATVDIDCDDSFDMEVRLVRGQPELDAPNMAILRMIDDDLELCDGGQAPRYYIA
                     GEQLGCTGAYSWQSDLEYVLGSWGGFFAIEPYSVDSSYLAFQRDGHVGWIQLSFDMES
                     METVYLRVHRVLSICGATGLEEPYIALPVLFPNPTIEDEAFIDPGSGGVRSVEVLDAV
                     GRIVARYGTVRTIPAPRLPGSYLVRVTGADGRMTVLRWMRR"
BASE COUNT          295 a          442 c          533 g          335 t
ORIGIN      
        1 gctgcttctc gggaagaaat gagccgaagg ggaggaggaa ctgggagtgg atggtgacgc
       61 ccttcgcgtt gagcgcggcg atgcccgtgg gggccaggat gacgtaccgc ttgtgcgtgc
      121 tggccgccag cttgtgcagg aaggtggtct tgccggtgcc ggccttgccc gtgaggaaga
      181 cgtggcgatt cgtgctgttg acgaagcgcg cggccagtgc ggccatttcg gtctcaacgt
      241 gcgcgtcgga cgtggaggtt ccagggttca cttcgcgaag tacggaagtg ttctcatcaa
      301 gcggaaaggg catttttcat ggatctacgg tacggctccg gtggtcactg acgcgaaaag
      361 cgtcacccgg cgatggcaat gtagcaccga tgggagagga ggaggtgacc cgaacggggg
      421 tcaagcgtgg gcctgcgagg gtcgcgatgt tcccgtgagc aaaatgtggt cattcaggca
      481 gcggatcgac cggaagcacc gtcatgtagt tggttaccga acaaccgtta cgatgaagca
      541 ccgtatcgca ctcctgctct gcatagggat cggtctttcc gtgcgagccc aactcaccgc
      601 cggcgaagta cccgatggcc agatcgccta cgaccccggg atcgatctgg agctgagcat
      661 gcccaatagt gtggacagcg ccacggtcga tatcgattgt gatgactcgt tcgacatgga
      721 ggtccggttg gtgcgcggcc aacccgaact ggacgcaccg aacatggcca tcctccggat
      781 gatcgatgac gatcttgaac tgtgcgacgg tgggcaggcg ccacgctact acatcgccgg
      841 ggagcaattg ggttgcacgg gagcgtactc gtggcagagt gatctggaat acgtgctcgg
      901 ctcgtggggc ggcttcttcg caattgagcc atactccgtt gacagttcgt acctggcctt
      961 ccaacgcgat ggtcatgtcg gttggatcca gctttcattt gatatggaga gcatggaaac
     1021 cgtgtacctg cgggtgcatc gggtgctctc catctgcggg gccaccggcc tggaggagcc
     1081 gtacattgcg ttgccggtat tattccccaa cccgacgatc gaggacgaag cctttattga
     1141 tcctggttcc ggtggagtgc gttcggtgga ggtactcgat gccgtgggca ggatcgtagc
     1201 ccgctatggc acggtccgaa cgatccctgc gccaaggttg ccaggcagct acctggtccg
     1261 cgttaccgga gcggacggcc gtatgaccgt tcttcgctgg atgcgccggt gaggaagggt
     1321 catgccggtc gtcggttcct gctcggccta tgataatggg aaccggtatt catttcgcgg
     1381 gcttccggaa tgctttgttg catggccaat tccacggagt tgcccacgca cctcgtcaat
     1441 acggcaccgc caggcccacc ccgacgccca tgttgctgtt gtagtgcgtg cgcacggcca
     1501 agatcttgtc gatcattgcg cagggtggtc gtcctacggg ctggaaagga gccttgaccc
     1561 ccgcgatcag atcttcagca aacgggccga tcggcggtcc actcc
//
LOCUS       DAOQOC010000813         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_59879_length_1604_cov_2.975468, whole genome shotgun sequence.
ACCESSION   DAOQOC010000813 DAOQOC010000000
VERSION     DAOQOC010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59879_length_1604_cov_2.975468"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..491)
                     /locus_tag="PL106_12030"
     CDS             complement(78..491)
                     /locus_tag="PL106_12030"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNA33843.1"
                     /translation="MNKLREKKEEPGEAPKKKAAGKPARLPRALISVLNGSFLTKESV
                     LSNMPFILFCAGLGLAYIAYGYWTERTVRELDRTNGELKEMRSEYLTVRSHLEQAQQQ
                     SHVAGDISALGLKESRTPPMRITVEKDQLETPAVE"
     gene            complement(488..1399)
                     /gene="rsmH"
                     /locus_tag="PL106_12035"
     CDS             complement(488..1399)
                     /gene="rsmH"
                     /locus_tag="PL106_12035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNA33844.1"
                     /translation="MTTGSEYHDPVLLQDCVDALRIRKGGIHVDVTFGGGGHSRAILE
                     KLDPSGSLVAFDRDRDAWRNAPKDARFTLVRADFRWLKNHLRFLNKLPIDGLLADLGV
                     SSHQFDTGARGFSFRFDGPLDMRMDHRAKLSAADVVNSYDHARLAELLRTRGEVDNAM
                     RVAKAITSARSTKRITTTHQLLAALDPVTPKKDANSFRARVFQALRITVNDELGALEA
                     LLKQSADVIRPGGRLVVISYHSLEDRLVKNWMRTGDLGGEEKKDIYGNRTRPFDPDSN
                     KATQPTEEEMNRNPRARSARMRAATRT"
     gene            complement(1383..>1604)
                     /locus_tag="PL106_12040"
     CDS             complement(1383..>1604)
                     /locus_tag="PL106_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNA33845.1"
                     /translation="ATPVSPDPTGRMLLPYALMKDARMGNDLKLIGADDRIEVWDAKA
                     YADMRKDETVDMSSLSESVMGALGNDDGK"
BASE COUNT          234 a          510 c          519 g          341 t
ORIGIN      
        1 ggcgatcgcg agggcgaagg ccacgaggca gccttcgatc agcgctgcgc ggaggcggag
       61 gaccttgtcg gggggcatca ttccacagcg ggcgtttcca gctggtcctt ttccacggtg
      121 atgcgcatgg gcggcgtgcg gctctcctta aggccgagcg cactgatgtc gcccgccaca
      181 tggctctgtt gctgcgcctg ttccaggtgg ctgcgcacgg tgaggtactc gctgcgcatc
      241 tccttcaatt cgccgttggt gcggtccagt tcacgtaccg tacgctccgt ccagtagcca
      301 taggcgatgt aggccaggcc cagccccgcg cagaagagga tgaagggcat gttgctcaat
      361 accgattcct tggtgaggaa actgccgttg agcacgctga tgagggcgcg gggcaggcgt
      421 gcgggcttgc cggcggcctt ttttttcggg gcctcccccg gctcctcctt cttttcgcgt
      481 agtttgttca cgtccttgtg gctgctctca tgcgtgcgct gcgcgcacgc gggttccggt
      541 tcatctcctc ctcggtgggc tgtgtcgctt tgttgctgtc ggggtcgaac gggcgggtac
      601 gattgccgta gatgtccttc ttctcctctc cgccgaggtc gccggtgcgc atccagttct
      661 tcaccaggcg gtcctccagg ctgtggtagc tgatcaccac cagccttcca ccgggccgga
      721 tcacatcggc gctctgcttg agcaacgctt ccagcgcacc cagctcatcg ttcaccgtga
      781 tccgcagcgc ttggaacacc cgggcacgga aggagttggc gtccttcttc ggggtcaccg
      841 ggtccagcgc ggcgagcaac tggtgcgtgg tggtgatgcg cttcgttgac ctggcgctgg
      901 tgatcgcctt ggcaacacgc atcgcattgt ccacttcgcc ccgggtgcgg agcagctcgg
      961 cgagccgtgc gtggtcgtag ctgttcacca cgtccgccgc gctcagcttg gccctgtggt
     1021 ccatgcgcat gtcgagcgga ccatcgaagc ggaagctgaa tcctcgcgcg ccggtatcga
     1081 actggtggct gctcacgccg aggtcggcca gcagtccatc gatcggcagc ttgttcagga
     1141 agcgcaggtg gttcttcagc cagcggaagt cggcacggac gagcgtgaag cgggcgtcct
     1201 tcggcgcgtt gcgccatgcg tccctgtcac gatcgaaggc gaccagcgaa ccgctgggat
     1261 ccagcttttc gagtatcgcc ctgctgtggc ccccaccgcc gaaggtgaca tccacgtgga
     1321 tgccgccctt gcggatcctc aaagcatcga cacagtcttg taaaagaacg ggatcgtggt
     1381 actcacttcc cgtcgtcatt gcccagtgcg cccatgacgg attcggacag ggagctcata
     1441 tccacggtct cgtccttgcg catgtcggcg taggccttgg cgtcccagac ctcgatccgg
     1501 tcgtccgcgc cgatcagctt gaggtcgttg cccatgcggg catccttcat caaggcgtac
     1561 ggcaacagca tgcgtccggt agggtccggc gacacgggcg tggc
//
LOCUS       DAOQOC010000814         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60046_length_1602_cov_2.744021, whole genome shotgun sequence.
ACCESSION   DAOQOC010000814 DAOQOC010000000
VERSION     DAOQOC010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60046_length_1602_cov_2.744021"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..>1602
                     /locus_tag="PL106_12045"
     CDS             199..>1602
                     /locus_tag="PL106_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33846.1"
                     /translation="MQHAPQRPAGNVFTWNGNQEAPDHTPTAAGITDQTAAQSGKDWL
                     MDHTTVTDLEGNVTGIVGVGYSFPQNWSYTNGCVVGSIGGFPDPLLQETHARRLGSVR
                     QVVCYYNASGILQWCKFLGAGVAHGVIVDREGYIVVTGQGYNQYPFPETPGLASLFNP
                     APGYTQPMDGFPCPSEDQMTLAKLDLSGNVVWSHFYNTQDDLNVASTERCQGQSLVET
                     NAGGVTGYRIVGWQRDVDNLGIQINQRPCLFQVDGNGLIQFKHRYPMIPGADSSPSSN
                     DQMRAMYISRCPFSVGERFAVTGYRTVDVTGIGTRTSAWLMYMDDAIGTVLDAEWTKD
                     VFTDNAYFGADLAFNNNANRVAVTMDGNTPCVAWPVMLNFQRRGSAGAPLVNADITAS
                     SANREATARLYKFNGTGGAPMWTVNPDLGPVRALDLFYSVKQRNSGNLVVASTKLSPG
                     FSIDNPLDYADMPTDVQN"
BASE COUNT          353 a          474 c          483 g          292 t
ORIGIN      
        1 ataaaaccgg aagtacaatg agcgatcaac ggacaagtgg accagggggt gggcctgccc
       61 tgctcaaagg agcgctaaag ggcgccggaa gccgcgccag atgggcgaaa acgaaagtgg
      121 gggggtaatt tccttgcgct cgcgatgaac gcgcctgagc gaagtgcagg gcaaacggga
      181 gcggtacttt ggcagaccgt gcaacacgct ccccaacgcc cggccggaaa tgtcttcaca
      241 tggaacggga atcaggaggc cccggaccat acccctaccg cagcagggat aacggatcag
      301 accgcagccc aaagcggtaa ggattggctg atggaccata ccacggtgac cgaccttgaa
      361 ggcaacgtga cgggcattgt cggggtagga tactctttcc cacagaattg gagctacacg
      421 aatggctgtg tggtgggttc tatcggcggc tttcccgatc ctttgttgca agaaacgcac
      481 gcccgacgct taggctcggt gcgccaagtg gtctgttact acaatgcgtc ggggatcctc
      541 cagtggtgca agttcctggg cgcgggcgtg gcccatggcg tgatcgtgga ccgcgaggga
      601 tacatcgtcg tcactgggca gggctacaac cagtatccct tcccggaaac acccgggctc
      661 gccagcctgt tcaatcccgc accgggctac acccagccca tggatggttt cccttgcccc
      721 agcgaggacc aaatgaccct ggccaagctg gatctttccg ggaatgtggt ctggagccat
      781 ttttacaaca cgcaagatga tctgaacgtg gcctccaccg aacgctgcca gggccagagc
      841 ctggtggaga ccaacgccgg tggcgtcacc ggatatcgga tcgtgggttg gcagagagat
      901 gtggacaacc tgggtattca gatcaaccag cgcccctgcc tgttccaagt ggacggcaac
      961 ggactgatcc agttcaagca ccgctatccc atgatacctg gtgccgacag cagcccatcg
     1021 agcaatgacc agatgcgggc catgtacatc agtcgctgcc ccttcagtgt cggggaacgt
     1081 ttcgcggtaa cgggttatcg caccgtggac gtaacgggga tcggtacgcg tacgagcgct
     1141 tggttgatgt acatggacga cgctatcggt accgttctgg atgccgagtg gaccaaggat
     1201 gtcttcaccg ataacgccta cttcggcgcc gatctcgcct tcaacaacaa cgccaaccgg
     1261 gtggccgtga ccatggacgg caacacacct tgcgtcgcat ggccggtgat gctcaatttc
     1321 cagagaagag gaagtgcagg tgctccgctc gtgaacgctg acatcactgc ttcctccgcg
     1381 aatcgcgagg ccacggcacg gctgtacaaa ttcaatggca cgggaggcgc ccccatgtgg
     1441 acggtgaacc ctgacctggg tcccgtgcgc gccttggacc tgttctactc cgtgaagcag
     1501 cgcaactccg gcaacctcgt tgtggccagt acaaaactct ccccggggtt cagcattgac
     1561 aaccccctgg actatgccga catgcccaca gacgtgcaga ac
//
LOCUS       DAOQOC010000815         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60111_length_1601_cov_3.038163, whole genome shotgun sequence.
ACCESSION   DAOQOC010000815 DAOQOC010000000
VERSION     DAOQOC010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60111_length_1601_cov_3.038163"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PL106_12050"
     CDS             complement(<1..1583)
                     /locus_tag="PL106_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNA33847.1"
                     /translation="MATGMAEHDRYAIDFIEGVRWIKQNLPGALTSGGVSNVSFSFRG
                     NEPVREAIHTAFLYHAIKAGLDMGIVNAGQIGVYDDIPKDLLEHVEDVLLARRPDATE
                     RMVAFAEQFKGAPSADVIAAQAAWREGTVEERLKHALVHGITDFIEVDTEEARVKYVD
                     PVIVIESPLMAGMSVVGDLFGSGKMFLPQVVKSARVMKRAVAYLEPFLEDKKAGQVMG
                     SMKADAKKVLLATVKGDVHDIGKNIVGVILACNGWQVIDLGVMVQSATILKTAREMKV
                     DIIGLSGLITPSLDEMVHVAKEMEREGFETPLLIGGATTSRVHTAVKIAPHYSKPVVH
                     VLDASRSVPVVSNLLSDNERARFEVEVKEEYEKVRTQYEGHQRDKEYVPLAEAREKKF
                     AIDFAAEPPVAPKSTGIFTYRNFPLADLVPYIDWTPFFMSWELAGKFPKILEDAVVGQ
                     QATQLYNDARTMLDRMVKEQWVQAHGVVGIWHANSDAQDDMEVYGDAARTKVIGRFHT
                     MRQQSKKAPGVPYIALSDF"
BASE COUNT          259 a          529 c          473 g          340 t
ORIGIN      
        1 atgaagtccg agagggcgat gtagggcacg ccgggtgcct tcttgctctg ctgccgcatg
       61 gtgtggaaac ggccgatcac tttggtgcgt gccgcgtcgc cgtacacctc catatcgtcc
      121 tgcgcatcgc tgttcgcgtg ccagatgccc accacgccgt gcgcttgcac ccactgctcc
      181 ttcaccatgc ggtccagcat ggtgcgtgca tcgttgtaca actgcgtggc ctgctggccc
      241 accacggcat cctccaggat cttcgggaac ttgcccgcca gctcccagct catgaagaag
      301 ggcgtccagt cgatgtaggg caccaggtcg gcgagcggga agttgcggta ggtgaaaatg
      361 ccggtgctct tgggcgctac cggtggttcg gcagcgaagt cgatcgcgaa cttcttctcg
      421 cgcgcctcgg ccagcggtac atattccttg tcgcgctggt ggccttcata ctgcgtacgc
      481 accttctcgt attcttcctt cacttccacc tcgaaacgcg cacgttcgtt gtcgctgagc
      541 aagttgctca ccacgggcac gctgcgcgaa gcatcgagca catgcaccac gggcttgctg
      601 tagtgcggcg cgatcttcac ggcggtgtgt acgcgactgg tggtggcacc accgatcagc
      661 agcggcgtct cgaaaccttc gcgctccatc tccttcgcca cgtgtaccat ctcatcgagc
      721 gaaggcgtga tcaagccgct gaggccgatg atgtccacct tcatctcgcg ggcggtcttc
      781 aggatggtgg cgctctgcac catcacgccg aggtcgatca cctgccagcc gttgcaggcg
      841 aggatcacgc ccacgatgtt cttgccgatg tcatgcacgt cgcctttcac cgtagcgagc
      901 agcaccttct ttgcatcggc cttcatgctg cccatgacct gtccggcctt cttgtcctcc
      961 aggaagggtt cgaggtaggc cacggcgcgc ttcatcacgc gggcgctctt caccacttgg
     1021 ggcaggaaca tcttgcctga tccgaacaga tcgcccacca cgctcatgcc ggccatcagc
     1081 ggactttcga tcacgatcac gggatccacg tacttcacac gggcctcttc ggtatccacc
     1141 tcgatgaagt cggtgatgcc atgcaccagc gcgtgcttca ggcgttcctc cacggtgcct
     1201 tcgcgccatg cggcttgtgc cgcgatcacg tccgcactcg gcgcaccctt gaactgctcg
     1261 gcaaaggcca ccatacgttc ggtggcatcg gggcgacgcg ccagcagcac atcctccaca
     1321 tgctccagca ggtccttcgg gatgtcgtcg tatacaccga tctgcccggc gttgacgatg
     1381 cccatgtcga gccccgcttt gatggcgtgg tagaggaagg ccgtgtggat ggcctcacgc
     1441 accggctcgt tgccgcggaa gctgaagctg acgttgctca cgccgccgct ggtcaaggcg
     1501 ccgggcaggt tctgcttgat ccagcgcacg ccttcgatga agtcgatggc gtagcgatcg
     1561 tgctcggcca tgccggtggc cacggtgagg atgttcgcgt c
//
LOCUS       DAOQOC010000816         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60265_length_1599_cov_5.340026, whole genome shotgun sequence.
ACCESSION   DAOQOC010000816 DAOQOC010000000
VERSION     DAOQOC010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60265_length_1599_cov_5.340026"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PL106_12055"
     CDS             <1..945
                     /locus_tag="PL106_12055"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33848.1"
                     /translation="LRSFLLAHGPLSEEALIAAIDREEPMDPWHLTQVLIANSKLSGP
                     IWAELELTGALSPFFYQMVRDHQEDPSIRQILMDELALRAYEKDTEQRLLVLALQEDS
                     TYTGKLDTLLAILSTDTLGMGRTAAYQLALAHGRSTEAAMLATDLVGDARFEQLLDLG
                     SLQQGLGADWSQAGQSELSSLQSMAATGKQLGRGAAWGILYALGATDSLPTGILPMEY
                     RTARVDEANADPTGRPVVGAYPNPAKDRLMITWPAEADGGALEVTDAQGRVVLTQSLA
                     GHTAFLELDVRAWADGLYLARVLRDDRVVGETKCAIVR"
     gene            942..>1599
                     /locus_tag="PL106_12060"
     CDS             942..>1599
                     /locus_tag="PL106_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33849.1"
                     /translation="MRRAWLFAFSILAGSIAPCQDTFIVQFDSVQAGHDASGRSVMET
                     DSGLIVFAWQHSDDGTGRMRSAVYELAADGTFVSRYEIGSGDSTTSGFGNFDPVERLV
                     DGGFISTITNHQGYSYAIALARFNANGDLIGSTPVISSPPEDSTYIFSRQLRRTSDGG
                     FVFCGFVDPPDDLAKASLVKMDSAGVVEWQQVFTASGQLYDAISVAPYVDGGYVLAGY
                     R"
BASE COUNT          296 a          469 c          514 g          320 t
ORIGIN      
        1 ttgcgcagct tcctgctggc ccatggccca ctgagcgagg aggcccttat cgcggccatc
       61 gatcgggagg aacccatgga cccctggcac ctcacccagg tgctcatcgc caacagcaaa
      121 ctctcgggcc ccatctgggc cgaactggag ctaaccggag cgctgtcgcc cttcttctac
      181 cagatggtgc gcgatcacca ggaggacccc agcatccgcc agatcctgat ggacgagttg
      241 gccctgcggg cctacgagaa agacacggag cagcggttgt tggtgctggc tttgcaggaa
      301 gacagcacct acaccggcaa gttggatacc ctgctggcca tcctcagcac ggataccttg
      361 ggcatgggcc gaacagcggc ctaccaactg gcgctggccc atgggcgcag caccgaggcc
      421 gccatgctgg ccaccgacct ggtcggcgat gcgcgttttg agcaattgct cgaccttggt
      481 agcttgcaac agggccttgg tgccgactgg tcgcaggccg ggcagagcga actatcgtct
      541 ctgcagtcca tggccgccac cggcaagcaa ctggggcgtg gtgcagcttg gggcatcctg
      601 tacgccttgg gcgccaccga cagcctgccc accggcatac tgcccatgga gtaccgcacg
      661 gcacgggtgg atgaggcgaa tgccgacccg accgggcgac ccgtggtggg ggcttacccc
      721 aatccggcca aggaccgact gatgatcacc tggcccgctg aggcggacgg tggcgccttg
      781 gaggtgaccg atgcccaggg ccgcgtagtg ctgacacaaa gccttgcggg acatacggcc
      841 ttcctggaat tggatgtgcg cgcgtgggcc gatggcttgt acctggcgcg tgtgttgcgc
      901 gatgaccgcg tggtgggcga gaccaaatgc gcgatcgtgc gatgaggcgc gcctggttgt
      961 tcgcgttttc gatcctggca gggagcatcg ctccctgcca ggataccttc atcgtgcagt
     1021 tcgatagcgt acaggcgggt catgatgcca gtgggcgatc ggtgatggag actgatagtg
     1081 ggctcatcgt gttcgcatgg caacattccg atgatggtac gggccgcatg cggagtgcgg
     1141 tctacgaact tgctgcggat ggcacgttcg tgagtcgata cgagatcggt tcaggggact
     1201 cgaccacttc cggcttcggg aatttcgatc ctgtcgaacg gctcgtcgat ggaggattca
     1261 tctccactat cacgaatcac caaggctatt cctatgcgat cgcattggca cggttcaatg
     1321 ccaacggaga tctgatcgga agtaccccgg tgatctcatc acctccagag gattcgacct
     1381 acatattctc acgtcaattg cgaaggacct cggatggtgg tttcgttttc tgcgggttcg
     1441 ttgatccgcc tgacgacctt gccaaggcat cattggtgaa aatggacagc gcaggtgtgg
     1501 ttgagtggca acaggtcttt actgcgagtg ggcagttata cgatgccatc agcgtggcgc
     1561 cctacgtgga cggcggctat gtactggccg gctaccgct
//
LOCUS       DAOQOC010000817         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60605_length_1595_cov_3.671429, whole genome shotgun sequence.
ACCESSION   DAOQOC010000817 DAOQOC010000000
VERSION     DAOQOC010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60605_length_1595_cov_3.671429"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PL106_12065"
     CDS             complement(<1..1061)
                     /locus_tag="PL106_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNA33850.1"
                     /translation="MRKLLILPVLCVSAVLLAQQPKINGPGADHVVGDLLIGLQKGSD
                     VQAVMRDLLRAPDAPPGLVVDRVVSLPANIWLLKHDPDFPGARALEMVRRHPAVVAAQ
                     FNHLVHERITPNDSQFSQQWHHVDPQDNDIDSDLAWNITTGGNTALGDTIVVCVVEGF
                     DRSHSDLNANAWVNRAEIDGNGIDDDANGYVDDVFGWNPSAGNDNSLFSGSHGTSCAG
                     MVGAKGNNANGVSGANWAVKMMPVRIGSLTEANVIASYSYALAQRQRWNDTNGLEGAF
                     VVATSSSWGIDQADPDNYPLWCGFYDTLGEAGILNCGATTNSNLNVDVVGDMPTACSS
                     PYMISVTATNSSDQRTFSG"
     gene            complement(1094..>1595)
                     /locus_tag="PL106_12070"
     CDS             complement(1094..>1595)
                     /locus_tag="PL106_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33851.1"
                     /translation="GTVLRSRTALLQRDGDVVSIDGVSPVQFDLPASSYHIAVRHRNH
                     LGVMTGAAVALSNVPTSIDLTSPAFGTYGSDARKAIGAVRALWAGNAFADGTLKYTGG
                     GNDRDPILLAVGSIAPNNSIAGYRTEDVNLDGIVKYTGNGNDRDPILVNLGSTTPNNS
                     RPEQLP"
BASE COUNT          277 a          492 c          498 g          328 t
ORIGIN      
        1 tatccgctga aggtgcgctg gtcgctgctg ttggtggcgg tgacgctgat catgtacggg
       61 ctgctgcagg ccgtgggcat atcgcccacc acatccacgt tcaggttgct gttggtggtg
      121 gcgccgcagt tgaggatgcc cgcttcgccc aaggtatcat aaaagccgca ccagagcggg
      181 tagttgtccg ggtccgcctg atcgatcccc caactgctgc tggtggccac cacgaacgcg
      241 ccttccaaac cgttggtatc attccagcgt tggcgctggg caagggcata ggaataactg
      301 gcgatcacgt tggcctccgt gaggctgccg atccgcacgg gcatcatttt tacggcccaa
      361 ttcgccccgg aaacgccgtt cgcgttgttc cccttcgctc ctaccatgcc ggcgcaactc
      421 gttccgtggc ttccgctgaa aagcgagttg tcgttgccgg ccgagggatt ccatccgaag
      481 acatcgtcca cgtagccgtt cgcatcatcg tcgatcccgt tcccgtcgat ctccgcacga
      541 ttcacccacg cgttggcgtt caggtcgctg tgactccggt cgaacccctc caccacgcac
      601 accacgatgg tgtcgcccaa ggccgtgttg ccccctgtgg tgatgttcca ggccaggtcg
      661 ctgtcgatgt cgttgtcctg cgggtccaca tgatgccact gctggctgaa ctggctgtcg
      721 ttcggtgtga tccgctcatg taccaggtga ttgaactgag cagctacgac ggcaggatgt
      781 ctgcgcacca tttccaatgc gcgggctccc ggaaagtccg ggtcgtgctt caataaccag
      841 atgttcgctg gcaggctcac cacccgatcc accaccaggc cggggggcgc atccggtgcg
      901 cgaagcaggt cgcgcatcac ggcctgcaca tcacttccct tctgcaggcc gatgagcagg
      961 tcgccgacca cgtgatcggc cccgggaccg ttgatcttgg gctgctgtgc caggagaaca
     1021 gcggagacgc agagtacagg aaggatgagc agcttgcgca tggtcgtggt gttcagtcgg
     1081 gtgggtatgg ccctcagggc agttgttcgg ggcgcgaatt gttcggggtg gtacttccca
     1141 ggttcaccag gatgggatcg cgatcattac cgttccccgt gtatttcacg ataccatcca
     1201 ggttcacatc ctcggtccgg tatccggcga tcgagttgtt cggtgcgata ctgccaacgg
     1261 ccagcaggat cggatcgcgg tcgttgccgc caccggtgta cttcaacgtg ccgtcggcaa
     1321 acgcattacc ggcccagagc gcccgtacag caccgatcgc cttgcgcgca tccgagccgt
     1381 acgtaccgaa ggccgggctg gtgaggtcga tggaggtggg cacattggag agtgcgaccg
     1441 cagcgccggt catcacaccc aggtggttgc gatgccgtac cgcgatgtgg taactggagg
     1501 caggaagatc gaactgtacc ggtgatacac catcgatgga gaccacatcg ccgtcgcgtt
     1561 gcaacagggc cgtgcgcgag cggagcacgg tcccg
//
LOCUS       DAOQOC010000818         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60828_length_1592_cov_3.522446, whole genome shotgun sequence.
ACCESSION   DAOQOC010000818 DAOQOC010000000
VERSION     DAOQOC010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60828_length_1592_cov_3.522446"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1592
                     /gene="porU"
                     /locus_tag="PL106_12075"
     CDS             <1..>1592
                     /gene="porU"
                     /locus_tag="PL106_12075"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNA33852.1"
                     /translation="IPSYQSYDSWVPSSCFTTDDYFCLLDDNEGEANGDIVDVGVGRL
                     PISSLSQAREVVDKILNYDRLMLNSVQGNSCANGSDGGAADWRNWTVFVSDDQTGDGC
                     DNHIHMNNSNLLANLVESLRPCLNVNKIYLDAYMQVSTPGGQRYPEAQEVLRSRVQKG
                     ALVVNYVGHGGEVGWAHERLLDVETILGWTNFERLPLFVTATCEFTRWDDPARTSAGE
                     YVMLNPHGGGIALMTTTRIAYSGANQNLAMDFYTHVFQRQDEQGHAMRLGDIYRRTKV
                     DVAGEGNYRNFALLGDPSIRLAMTRQSARITAITDTLGNPLDTLMARATVRITGEVTD
                     TSGQVLTDFNGTVLPTVFDKLTALSTLDNDHCAGPYNYTLRKNIIYRGRATVTNGQFT
                     TTFVVPQDINYAVGPGRISIYAESLSDNACGYDNSPLVGASDPNATADANGPVIELYM
                     NDETFVPGGITDEKPLLLARLHDDNGINTLGSSIGHDLTATIDQSTENAIVLNDVYEA
                     DKDTYKSGTVRYRLSNLAEGSH"
BASE COUNT          332 a          511 c          492 g          257 t
ORIGIN      
        1 ggatcccgtc ctatcaatcg tacgactcgt gggtcccttc ctcttgcttc accaccgacg
       61 attatttctg cctgctggac gacaatgagg gcgaagccaa cggggacatc gtggatgtgg
      121 gcgtgggccg cctgccgatc agcagccttt cgcaggcgcg cgaggtggtg gacaagatcc
      181 tgaactacga ccggctgatg ctcaactcgg tgcagggcaa ctcgtgcgcg aacgggtccg
      241 atgggggagc ggcggactgg cggaactgga ccgtgttcgt gagcgatgac cagaccgggg
      301 acggatgcga caaccacatc cacatgaaca acagcaacct gctggcgaac ctggtggagt
      361 ccctccggcc ctgcctcaac gtcaataaaa tataccttga cgcgtacatg caggtgagca
      421 cgcccggagg gcagcggtat cccgaagcac aggaggtgct gcggtcacgc gtgcagaagg
      481 gcgcgctggt ggtgaattac gtgggccacg gtggcgaggt gggatgggcg cacgaacgcc
      541 tgctggacgt ggagacgatc ctgggatgga ccaacttcga gcggttgccc ttgttcgtta
      601 ccgccacgtg cgaattcacc cgctgggacg atccggcgcg aacatcggcc ggcgagtacg
      661 tgatgctcaa tccccatggg ggtggtatcg ccctgatgac cactacgcgg atcgcgtaca
      721 gcggcgccaa ccagaatctc gccatggact tctatacgca cgtgttccag cgccaggatg
      781 aacaggggca cgccatgcgg ctgggcgaca tctaccgccg gacgaaggtg gacgtggccg
      841 gagagggcaa ctaccgcaat ttcgccctgc tcggcgaccc gtcgatccgc ctggccatga
      901 cccgccagag cgcccggatc accgccatca ccgacacctt gggcaacccc ctggataccc
      961 tgatggcccg ggccaccgtg cgcatcaccg gggaagtgac ggacacctcc gggcaggtgc
     1021 tcacggattt caatggcacc gtactgccca cggttttcga caagttgacg gcgttgagca
     1081 ccttggacaa tgatcactgc gccgggccgt acaactacac cctccgcaag aacatcatct
     1141 accgcggccg tgccaccgtg accaacgggc agttcaccac caccttcgtg gtaccgcagg
     1201 atatcaacta tgcggtcggg ccgggccgga tcagcatcta tgccgagagc ttgtcggaca
     1261 atgcctgcgg gtacgacaac tcaccactgg tgggcgcctc ggaccccaac gccacggccg
     1321 acgccaacgg gccggtgatc gagttgtaca tgaatgacga gaccttcgtt cccggcggca
     1381 tcaccgacga gaagcccttg ctcctggctc ggttgcacga cgacaacggc atcaacaccc
     1441 tgggcagcag catcggccac gatctgaccg ccacgatcga ccagagcacg gagaacgcca
     1501 tcgtgctcaa cgacgtgtac gaggcggaca aggacaccta caagagcggc actgtgcgct
     1561 accggctcag caacctcgcc gaaggatccc ac
//
LOCUS       DAOQOC010000819         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60838_length_1592_cov_3.205595, whole genome shotgun sequence.
ACCESSION   DAOQOC010000819 DAOQOC010000000
VERSION     DAOQOC010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60838_length_1592_cov_3.205595"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PL106_12080"
     CDS             complement(<1..731)
                     /locus_tag="PL106_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNA33853.1"
                     /translation="MTILGASETRAQDPQFSQFYAAPLYLNPALTGNTFQDRLILNYR
                     KQWLGIAPGFETYALSYDHNNPKLHSGFGGMVMRDVAGTSGLSFTKAAFSYSYEARID
                     HKRAVRFGTSLGYTLRQLGQGQLIFADQILREDPNSLEGDRLRMNTSYFDAGFGAMYF
                     SEAFWIGLSLNHVNRPQQSLYLDGDVRLPVRTSIHTGYRIATDGQKLRRSRQYVTIAA
                     HYKAQDKWDQLDVGAYFDHEKLTVGL"
     gene            complement(848..>1592)
                     /locus_tag="PL106_12085"
     CDS             complement(848..>1592)
                     /locus_tag="PL106_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA33854.1"
                     /translation="QFPDSLTTQNVTWLWNFGDGTTSTAPAPVHTYAPGSYTVSLQVA
                     TPAGCTANAQNTSSVTAYQGPTASFGASTYQTDMDAPTITFTSTSAGAINGYAWEFGD
                     GDTSNTDNPVHTYAAVGNYPVQLVVTDVNGCSDTANTGINVIPVYDIDIPTAFTPDPN
                     GNNNGVFDPNDMSNDVFYPIARFVKEFQMRVWNRWGELVFESNEVNKGWNGFYRGRLS
                     PQDVYVYHAKIRFVDDRVAERNGDITLLR"
BASE COUNT          272 a          462 c          511 g          347 t
ORIGIN      
        1 aggcctacgg tcagcttttc atggtcgaaa taggccccca catccagctg gtcccacttg
       61 tcctgtgcct tgtagtgcgc ggcgatcgtt acatattgcc ggcttcgacg cagcttctgg
      121 ccgtccgtgg cgatccggta gcccgtatgg atgctcgtac ggaccggcag gcgtacatca
      181 ccatcgagat acagcgactg ctgtggccgg ttcacatggt tcaaactcag gccgatccag
      241 aaggcctcac tgaagtacat ggcgccgaat cccgcgtcga agtagctcgt attcatgcgc
      301 aggcggtcgc cttcgaggct gttcgggtcc tcgcggagga tctggtcggc gaagatcaac
      361 tggccctgac cgagttggcg caaggtgtag cccaggctgg tgccgaagcg taccgcgcgt
      421 ttgtgatcga tacgggcctc gtagctgtag ctgaaggccg ctttggtgaa ggacaagcca
      481 ctggtgccgg ccacatcgcg catcaccatg cccccgaagc cgctgtggag cttggggttg
      541 ttatggtcat aactgagcgc ataggtctcg aagccgggtg cgatacccag ccattgcttc
      601 ctatagttga ggatcaaccg gtcctggaag gtgttgcccg tgagtgcggg attcaagtac
      661 aggggtgcgg cgtagaattg cgagaattgc gggtcctgcg cgcgggtctc cgacgctccg
      721 aggatggtca tgaacaacgc ggcgcaggcc atcgcgatcc gggagctgcc ctccgcgcgg
      781 cgccaccggc gtgatccgtg ccggagcccg caggccgtat gttccacgtt gatcctcatc
      841 cgatccatca gcgcaacagg gtgatatctc cgttccgctc ggccacacgg tcatccacga
      901 agcggatctt cgcgtggtac acgtacacgt cctgagggct caagcgaccg cgatagaagc
      961 cgttccagcc cttgttcacc tcgttgctct caaagacgag ttccccccaa cggttccata
     1021 cgcgcatttg gaattccttc acgaatcgcg cgatggggta gaacacgtcg ttgctcatgt
     1081 cgttcgggtc gaacacaccg ttgttgttcc cgttcgggtc cggggtgaac gccgtgggga
     1141 tgtcgatgtc gtagaccggg atcacattga tgccggtgtt ggccgtgtcc gagcagccgt
     1201 tcacgtccgt aaccaccagt tgcacggggt agttgcccac cgcagcatag gtgtgtaccg
     1261 ggttgtcggt gttgctggtg tccccatcgc cgaattccca cgcatatccg ttgatcgcgc
     1321 ccgcactggt gctggtgaat gtgatggtgg gggcatccat gtccgtttgg taggtgctcg
     1381 cgccgaacga ggctgtcgga ccttgatacg ccgtcacgct ggaagtgttc tgtgcgttcg
     1441 cggtacaacc cgcgggggtg gccacctgca ggctgaccgt gtagctgccc ggggcatacg
     1501 tgtgtacagg tgcgggagcc gtgcttgtgg tgccgtcccc gaagttccaa agccaggtga
     1561 cgttctgggt ggtcaggctg tcggggaatt gt
//
LOCUS       DAOQOC010000820         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_60913_length_1591_cov_3.957031, whole genome shotgun sequence.
ACCESSION   DAOQOC010000820 DAOQOC010000000
VERSION     DAOQOC010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60913_length_1591_cov_3.957031"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PL106_12090"
     CDS             complement(<1..726)
                     /locus_tag="PL106_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HNA33855.1"
                     /translation="MAFFGLFGKKKEAKEEAAEKEALDSGLQRSREGFLGRIARAVAG
                     KSQVDDEVLDQLEETLVQSDVGVETTLRIIGRIQERVKRDKYVGTAELNGILRDEIAA
                     LMKDPAPVDPAIKPYVIMVVGVNGVGKTTTIGKLAHAFKKEGRSVLLGAADTFRAAAV
                     DQLRIWSERVGVPLVQQGMGADPAAVAYDTVESAKARGAEVVIIDTAGRLHNKTSLMN
                     ELTKVRNVMRKVVPDAPHEVLLVL"
     gene            complement(815..973)
                     /locus_tag="PL106_12095"
     CDS             complement(815..973)
                     /locus_tag="PL106_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HNA33856.1"
                     /translation="MAKKVVATLKKADAKVFVKVIRMVKNDRTGGYAFAEQIMSQEDA
                     DKALSGKK"
     gene            complement(1018..1200)
                     /gene="rpmG"
                     /locus_tag="PL106_12100"
     CDS             complement(1018..1200)
                     /gene="rpmG"
                     /locus_tag="PL106_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNA33857.1"
                     /translation="MAKKGNRVQVIMECTEHKASGQPGTSRYVTTKNRKNTNERLELK
                     KYNPILRRMTVHKEIK"
     gene            complement(1251..1490)
                     /gene="rpmB"
                     /locus_tag="PL106_12105"
     CDS             complement(1251..1490)
                     /gene="rpmB"
                     /locus_tag="PL106_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNA33858.1"
                     /translation="MSRICQITGKKVMGGHSVSHSNVKTKRTFAPNLQDRKYYLEAEK
                     RWVTLRVSVNGMRTINKIGLAEALKRAQAKGFYKA"
BASE COUNT          254 a          494 c          471 g          372 t
ORIGIN      
        1 caggacgagc aggacctcgt ggggcgcatc gggcaccact ttgcgcatca cattgcgcac
       61 cttggtcaac tcgttcatca ggctggtctt gttgtgcagg cggccggcgg tatcgataat
      121 gaccacctcg gcacccctgg ccttggcgct ctccacagtg tcgtaggcca cggcagcggg
      181 gtcggcgccc atgccttgct gcaccagcgg aacgccgacc cgttcgctcc agatgcgcaa
      241 ctggtccacg gcggcggccc ggaacgtatc ggccgcaccg agcagcacgc tcctgccctc
      301 cttcttgaac gcgtgggcca gcttgccgat ggtggtcgtc ttgcccacac cattcacgcc
      361 aaccaccatg atcacgtacg gcttgatggc ggggtcaacg ggtgctggat ccttcatcaa
      421 cgccgcgatc tcgtcgcgca ggatgccgtt gagttcggcc gtgcccacgt acttgtcgcg
      481 cttcacccgc tcctggatac ggccgatgat gcgcaacgtg gtctccacgc ccacatcgct
      541 ctgcaccagt gtttcctcca actggtccag tacctcatcg tccacctggc tcttgccggc
      601 cacggcgcgg gcgatgcggc ccaagaagcc ttcgcgcgag cgctgcagcc cactgtcgag
      661 cgcttctttc tcggccgctt cctccttggc ctctttcttc ttgccgaaca gtccgaagaa
      721 tgccatgggc tacgggaatt cgggatgtac cggggtcaag gcgtgcgaaa ggcttccctc
      781 ctcgcggcgg aaagccttca cgacagggat atgatcactt cttgccggag agggccttat
      841 cggcatcttc ctgcgacatg atctgctcgg cgaacgcgta accacccgtg cggtcgttct
      901 tcaccatgcg gatcaccttg acgaacacct tggcgtccgc tttcttcagg gtagcaacta
      961 ccttctttgc catgacttat gcgcttaata gctggttgtt gtgcgcggag gcgcggctta
     1021 cttgatctcc ttgtgaacgg tcatacggcg caggatggga ttgtacttct tcaactccaa
     1081 acgctcgttg gtgttcttgc ggttcttggt ggtgacgtag cggctggtgc cgggctggcc
     1141 ggacgccttg tgctcggtac actccatgat cacctgtacg cggttgcctt tcttggccat
     1201 tgttcaatcg ccgtagcccg gcgaaggcgt tgttcagcgt gttgcggggt tcaggccttg
     1261 tagaagcctt tggcttgggc ccgcttgagg gcttcggcca ggccgatctt gttgatggtg
     1321 cgcatgccgt tcacactcac acgcagggtc acccagcgct tttcggcttc caggtaatac
     1381 ttgcgatcct gcagattggg cgcgaatgtg cgcttggtct tcacgttgga gtggctcaca
     1441 ctgtggccac ccatcacctt tttgccggtg atctgacaga tacgggacat ggctttccgg
     1501 attcggggcg cgaatatgcg gaaaaattcc ggtcggttca tccaggaaga aatcatcatg
     1561 cgcaggggtg cgggtgcgtg gccgactgta c
//
LOCUS       DAOQOC010000821         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61089_length_1589_cov_4.488266, whole genome shotgun sequence.
ACCESSION   DAOQOC010000821 DAOQOC010000000
VERSION     DAOQOC010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61089_length_1589_cov_4.488266"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PL106_12110"
     CDS             complement(<1..525)
                     /locus_tag="PL106_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNA33859.1"
                     /translation="MASRNPAAVAVRVPDPRTAPRIDQVGIEERVARIKARSIKTGAK
                     TRGMKLALSMIDLTTLEGADTPNKVRQLCYKAMHLHDTLPDLPTVAAVCVYPSLVKVA
                     KQALGNSGVKVASVSTAFPSGQAPRNVKVADTRFAVAEGADEIDMVISRGRFHSGDHQ
                     FVFDEIAAIKEACGP"
     gene            complement(550..1149)
                     /gene="nadD"
                     /locus_tag="PL106_12115"
     CDS             complement(550..1149)
                     /gene="nadD"
                     /locus_tag="PL106_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNA33860.1"
                     /translation="MNIGCLFGSFDPPHAGHLAIARHMLKAAKLDQVWLVVTPQNPFK
                     QDRVLSPEHQRLAMARLAVQGEAGIVASGFELDLPKPNYTADTLAFMRERWPHHDFSL
                     IIGSDNLAGLHTWKEPHTILDNHRVLVYPRPGIEGHMAVSDLLYHPSVTLVNDAPLMD
                     ISSTGVRVRLRNWKPVEGLIHPDVGLYIRQNSLYTGKTD"
     gene            complement(1146..>1589)
                     /locus_tag="PL106_12120"
     CDS             complement(1146..>1589)
                     /locus_tag="PL106_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNA33861.1"
                     /translation="KRAHEVDGQDYWFISAGEFQRRIEADEFVEWEEVYPGQYYGTLK
                     HELHRIWKDGRHPIFDVDVVGGLDLKEIFGPNALAMFIAPPSIPALEERLRQRGTESP
                     ESLAKRVAKAGHELTYADRFDTVVVNDDRDRACIEAYQHVYDFLT"
BASE COUNT          254 a          503 c          513 g          319 t
ORIGIN      
        1 ggggccacag gcctccttga tggcggcgat ctcgtcgaac acgaactgat gatcgccgct
       61 gtggaaacgc cctcggctga tcaccatgtc gatctcgtcg gcaccttcag ccaccgcgaa
      121 gcgggtatcc gccaccttca cgttgcgagg ggcctggccg ctgggaaagg cggtggaaac
      181 ggaagccact ttcacgccgc tgttgcccaa ggcttgtttg gccaccttca caagggaggg
      241 atagacgcac actgcggcca ccgtgggcag gtccggcagg gtatcgtgca ggtgcatggc
      301 cttgtagcag agctgacgca ccttgttcgg cgtgtcggcc ccctcgagcg tggtgaggtc
      361 gatcatgctc agggcgagct tcatgccccg ggtcttcgcc ccggtcttga tgctgcgggc
      421 tttgatccgg gccacacgct cctcgatgcc cacctgatcg atcctgggtg cggttcgcgg
      481 gtccggtacg cggacggcca ccgctgcggg gttccttgat gccatcccgc caaaggtatg
      541 cggggttggt cagtccgtct tgcccgtgta caggctgttc tgccggatgt acaggcccac
      601 atcggggtgg atcaggccct ccaccggttt ccagttccgc agccgcacac gcacccccgt
      661 gctggaaata tccatgagcg gtgcatcgtt caccagggtg accgacggat ggtacagcag
      721 gtcactgacg gccatgtgac cttcgatacc tggacgtggg tacaccagta cccggtggtt
      781 gtcgaggatc gtgtgcggct ccttccaggt gtgcagaccc gcgaggttgt cgctgccgat
      841 gatgaggctg aaatcgtggt gcggccagcg ttcgcgcatg aacgccagcg tgtcggcggt
      901 atagttgggc ttgggcaggt ccagttcgaa cccgctggcc acgatgcccg cctcgccctg
      961 cacggccaag cgggccatgg ccagccgctg atgctcgggg ctcagtacgc ggtcctgttt
     1021 gaacgggttc tgaggcgtga cgaccagcca cacctgatcg agcttggccg ccttcagcat
     1081 gtggcgcgcg atggccagat ggcccgcatg cgggggatcg aaggatccga agaggcagcc
     1141 gatgttcatg tcaggaaatc gtacacgtgt tgatacgcct cgatgcacgc gcggtcacga
     1201 tcgtcgttga ccaccacggt gtcgaaccgg tccgcatacg tgagttcgtg cccggctttc
     1261 gccacgcgtt tggccaggct ttccggcgat tccgtgccgc gctggcgcag ccgctcctcc
     1321 agagcgggta tcgatggcgg tgcgatgaac atggccaggg cgttgggccc gaagatctcc
     1381 ttcaggtcca gtccccccac cacgtccaca tcgaagatcg ggtgccggcc atctttccag
     1441 atgcgatgga gctcgtgctt cagggtgccg tagtactggc cggggtacac ctcctcccat
     1501 tccacgaatt cgtccgcttc gatccgccgc tggaattccc cggcgctgat gaaccagtag
     1561 tcctgcccgt ccacctcatg ggcgcgttt
//
LOCUS       DAOQOC010000822         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61304_length_1586_cov_5.626388, whole genome shotgun sequence.
ACCESSION   DAOQOC010000822 DAOQOC010000000
VERSION     DAOQOC010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61304_length_1586_cov_5.626388"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PL106_12125"
     CDS             complement(<1..795)
                     /locus_tag="PL106_12125"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNA33862.1"
                     /translation="MSTLQETIKIFGGTASKYLAESIAAACGERVGNVVLNRFSDGEF
                     QPSFEETVRGDVVYIVQSTFPPSDNLFELLMMVDAAKRASAKRIVAVMPYFGFARQDR
                     KDKPRVSIGAKLVANMLTAAGVDRIMTMDLHADQIQGFFEVPVDHLFASSIFVPYIKS
                     LNLPNLLMVAPDTGGTKRANAYGKHLGVDIAICYKQRKVANQIEKMTVIGEVKGKDVM
                     LVDDMCDTAGTLTKAADMMLEQGANSVRAICTHPVLSGEACDRIERS"
     gene            936..>1586
                     /locus_tag="PL106_12130"
     CDS             936..>1586
                     /locus_tag="PL106_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33863.1"
                     /translation="MEQFSENSWLLSTALKNTAMILRFCFSVSSIAIATSLAQGQMVP
                     PAQGQTLWDYHTGFMTEPGVSEFSEEEGSALSLHNKMMMQWAPRLFPSGDLHGITEAV
                     RQQMTRMLTHTRSGGGIVSNWSPLGPMDMPNYQNTGIPNSRNGTGQIHVITFDPGYGT
                     GNSTVYCGSSYGGMFKSTNGGDSWTSMTDHAFPFTPVSDIVVLDQDPQTLIVGTGGA"
BASE COUNT          307 a          471 c          477 g          331 t
ORIGIN      
        1 gctgcgctcg atccgatcgc acgcctcgcc gctgagcacg ggatgcgtgc agatggcccg
       61 gacgctgttg gctccctgct ccagcatcat gtcggcggcc ttggtaaggg tgccggccgt
      121 gtcgcacatg tcgtccacga gcatcacgtc cttgcccttc acctcaccga tcaccgtcat
      181 cttctcgatc tggttggcca cctttcgctg cttgtagcag atggcgatgt ccaccccgag
      241 gtgcttgccg taggcgttcg cgcgcttggt accgcccgtg tccggtgcta ccatcagaag
      301 gttcggcagg ttcaggctct tgatgtacgg aacgaagatg ctgctggcga acagatggtc
      361 caccggcacc tcgaagaagc cctggatctg gtccgcgtgc aggtccatgg tcatgatgcg
      421 gtccacgccg gcggcggtga gcatgttggc caccagcttc gcaccgatgc tcacccgggg
      481 cttgtccttg cggtcctgcc gggcgaagcc gaagtagggc atcaccgcca cgatccgctt
      541 ggcgctcgcc cgcttggcgg cgtccaccat catcagcagt tcgaacaggt tgtcgctggg
      601 cgggaatgtg ctctgcacga tgtacaccac atcgccccgc accgtctcct cgaaactggg
      661 ctggaactcc ccgtcgctga agcggttgag caccacattg cccacgcgtt caccacaggc
      721 cgcagcgatg ctttcggcca agtacttcga ggcggtacca ccgaagatct tgatggtttc
      781 ctggagggtg gacatggggt gattcgcgta ccagatcgcc tcggccccgg cagggcctcg
      841 cgaacgggcc gcgaatgtag cgatcctggg tggttttcaa cacctgttgg tggaggggtt
      901 gtggcgggac cagtcagcgg ggagagtcac agaatatgga acagttctcg gagaattcgt
      961 ggctactttc aacggcacta aagaacactg ccatgatcct tcgattttgc ttttccgtgt
     1021 ccagtatcgc cattgccacg tcgctggcgc aaggccaaat ggtaccaccg gcacaaggtc
     1081 aaaccttatg ggactatcac accggattca tgaccgagcc gggggtgtcc gaattctcag
     1141 aggaggaggg cagcgccttg agcctccaca acaaaatgat gatgcaatgg gctcctcggt
     1201 tgttcccaag tggagacctc catggcatca ctgaggcggt ccgccagcaa atgacccgca
     1261 tgttgaccca tacgcgtagc ggaggaggca tcgtatccaa ctggagtcca ttgggtccga
     1321 tggatatgcc gaactatcag aacactggta ttccgaatag cagaaatgga acagggcaga
     1381 tccatgtgat cacgttcgac cctgggtatg ggaccggcaa cagcacggtc tattgcgggt
     1441 ccagctatgg tggcatgttc aagagcacca atgggggcga ttcttggacg tcgatgaccg
     1501 atcatgcatt cccattcaca cccgtatcgg atatcgttgt gctcgaccag gacccgcaga
     1561 cactgatcgt tggtaccggc ggggct
//
LOCUS       DAOQOC010000823         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61409_length_1585_cov_3.016993, whole genome shotgun sequence.
ACCESSION   DAOQOC010000823 DAOQOC010000000
VERSION     DAOQOC010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61409_length_1585_cov_3.016993"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PL106_12135"
     CDS             complement(<1..396)
                     /locus_tag="PL106_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33864.1"
                     /translation="MIWRTEQATGHGPRATGHGPQASDRSSLRPAVWGLLLSLVPLSC
                     HVHYGTSGGEVPAGAKTISVALFDARAPLCTPQSAQTFTETLRDLLQAQTPLNLTREE
                     GDLQYEGAIIAFDVQPVSIQANETAALNRL"
     gene            complement(380..1582)
                     /locus_tag="PL106_12140"
     CDS             complement(380..1582)
                     /locus_tag="PL106_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNA33865.1"
                     /translation="MLLKRAAEQQVAPTDLSVLITGESGSGKEVMPKIVHEYSPRKHG
                     PFIAVNCGAIPEGTIDSELFGHEKGSFTGANEARKGYFEVADKGTIFLDEVGELPLGT
                     QVRLLRVLETGEFIRVGSSRAQKTSVRVVAATNVNMWQAVQKGNFREDLYYRLSAIPI
                     FLPPLRERVNDIHLLFRKFAVDSAERHRIKEPITLTAEAQDLLTNYRWPGNIRQLRNI
                     VEQMNVIEKDQRIINADKLRWYLPTVDAPLLPALVRKETGTDGTQGPNERELLYSVIG
                     RLQQDVHALKEHLGALMDGGTVPAPHPTAGLGDARSVLRDEFIVPQQGHAHAPVRIER
                     APVGEEETRDVEHTEVQESLSLVDKEKELIQKALKKHRGRRKAAADELGISERTLYRK
                     INEYDLED"
BASE COUNT          235 a          491 c          513 g          346 t
ORIGIN      
        1 gaggcggttg agcgcggcgg tctcgttcgc ttgaatgctc acgggctgca catcgaaagc
       61 gatgatggcc ccttcatact gcaggtcgcc ttcttcgcgt gtgaggttca gcggtgtctg
      121 ggcctgcagg aggtcacgca gcgtttcggt gaaggtctgc gcgctttgtg gtgtgcataa
      181 aggggcccgt gcatcgaaaa gggccacgct gatggtcttc gcccctgcgg gcacttcgcc
      241 accgctggta ccgtagtgga cgtggcagga gaggggcacg agggatagca gcaggcccca
      301 gaccgcaggc cgcaggcttg accggtctga agcctgtggc ccgtggcctg tggcccgcgg
      361 cccgtgaccc gtggcctgct cagtcctcca gatcatactc gttgatcttc cggtacagcg
      421 tgcgctcgct gatgcccagt tcgtcggcgg cggctttacg acggccccgg tgcttcttca
      481 gcgctttctg gatcagctcc ttctccttgt ccaccaggct gagcgattcc tgaacttcgg
      541 tatgctccac atcgcgtgtc tcctcttcgc ccacaggagc gcgctcgatg cgtaccgggg
      601 cgtgcgcgtg gccctgttgc ggcacgatga actcatcgcg cagcacggaa cgcgcgtccc
      661 ccagaccagc ggtcgggtgc ggtgccggta ctgtcccgcc atccatcaag gcgccgaggt
      721 gctccttcaa tgcgtgcaca tcctgttgca gcctaccgat caccgagtag agcaattcgc
      781 gctcgttcgg accttgcgtc ccgtcggtac cggtttcctt gcggaccaat gctggcagca
      841 agggcgcatc cacggtgggc aggtaccagc gtagtttgtc ggcgttgatg atgcgctggt
      901 ccttttcgat cacgttcatc tgctccacga tgttgcggag ctgtcgtatg ttacctggcc
      961 atcggtagtt tgtgagcagg tcctgcgcct cggcggtcag ggtgatgggc tccttgatgc
     1021 ggtggcgctc ggcgctgtcc acggcgaact tgcggaagag caggtggatg tcgttcaccc
     1081 gttcgcgcag cggaggcagg aagatgggga tcgcgctgag gcgataataa agatcctctc
     1141 ggaagttgcc tttttgtacg gcctgccaca tgttcacgtt ggtggcggcc accacgcgca
     1201 cgctggtctt ctgggcgcgg ctgctgccca cgcggatgaa ctccccggtt tcaagtacac
     1261 gcagcaggcg cacctgggtg cccaagggca gttcacccac ctcgtccagg aagatggtgc
     1321 ccttgtcggc cacctcgaag tagcccttgc gggcttcgtt ggcgccggtg aacgatccct
     1381 tctcgtggcc gaagagttcg ctgtcgatgg tgccctcggg gatcgcacca cagttcaccg
     1441 cgatgaatgg cccatgcttg cgcgggctgt attcatgcac gatcttcggc atcacttcct
     1501 tgccgctgcc gctctcgccg gttatgagca cgctgaggtc ggtgggcgcc acctgttgtt
     1561 cagcggcgcg cttcagcagc aggcc
//
LOCUS       DAOQOC010000824         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61656_length_1582_cov_3.809430, whole genome shotgun sequence.
ACCESSION   DAOQOC010000824 DAOQOC010000000
VERSION     DAOQOC010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61656_length_1582_cov_3.809430"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1582)
                     /gene="uvrC"
                     /locus_tag="PL106_12145"
     CDS             complement(<1..>1582)
                     /gene="uvrC"
                     /locus_tag="PL106_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNA33866.1"
                     /translation="VVPSEFEALLLENTLIKEHQPRYNILLRDDKSYPLIRIRNEHFP
                     RVEGMRNPVEDGSEYYGPFASGLGMNTMLRLVHGLYKLRTCNYDLSPANIAKGKFKRC
                     LEYHIGNCKAPCEGLRSEEEYNADMAEVRQLVKGRMSGLMRGMKAQMLRHAEALEFEE
                     AEELRVKMERLERYHAKSVVVNMDIGDVDVFGIAGDTAVLCVNYLRVVEGAVVHGITI
                     ELKRRLEESEQDLLQLAIAELRQRYRSTAPEAVVPFEPGIEVPGLTFTVPQRGDRRKL
                     LEMCERNARFHFLEKQKQERLTDPQAATDRLLEQMKQDLHLAELPRHIECFDNSNTQG
                     TDPVSACVVFKDGKPSRKDYRHFNIRTVEGPDDFASMEEALERRYSRLLTQGDPLPQL
                     VVVDGGKGQLHAAVNVFDRLGLRGRVALVGIAKRLEELYFPDDPVPLHIDKRSSTLRT
                     IQLMRDEAHRFGITHHRARRGKRIVRTALEDIPGIGPETAKKLLVRFGSMKGIAEAPY
                     EEVVREVGEKKAALVRAGE"
BASE COUNT          259 a          528 c          467 g          328 t
ORIGIN      
        1 cctcccctgc gcgcaccagc gccgccttct tctcccccac ctcgcgcacc acctcctcgt
       61 acggcgcttc ggcgatgccc ttcatgctgc cgaaacgcac caggagcttc ttcgccgtct
      121 ccggcccgat gccggggata tcctcaagcg ctgtgcgaac gatgcgcttg ccccgtcgcg
      181 cgcggtgatg cgtgatgccg aaccggtgcg cctcgtcgcg catcagctgg atggtgcgca
      241 aggtgctgct gcgcttgtcg atgtgcaggg gaacgggatc atcggggaag tacaattcct
      301 ccaggcgttt cgcgatgccc accagcgcca ccctgccacg caggcccagc cggtcgaaca
      361 cgttcacggc ggcgtgcaac tggcctttgc ctccgtccac caccacgagt tgcggcaggg
      421 gatcgccctg ggtgagcaga cgtgaatagc ggcgttccag cgcctcctcc atgctggcga
      481 agtcatcggg accctccacc gttcgtatat tgaaatgccg gtagtctttc ctgctgggct
      541 tgccatcctt gaacaccacg catgcgctca ccggatcggt accctgggtg ttgctgttgt
      601 cgaagcactc gatgtggcgg ggaagctccg ccaggtggag gtcctgcttc atctgttcca
      661 gcaggcggtc ggtcgcggcc tgcggatcgg tgaggcgctc ctgtttctgc ttctccagga
      721 agtggaaacg cgcgttgcgt tcgcacatct ccagcaattt cctgcggtca ccccgctgtg
      781 gcacggtgaa ggtgagtccg ggcacctcga tgccgggctc gaaagggaca acggcctcag
      841 gcgccgtgct ccggtaccgc tgacgcagct cggcgatggc gagttgcagc agatcctgct
      901 cgctttcctc cagccgacgc ttgagttcga tggtgatgcc gtgaacgacg gccccttcca
      961 ccacgcgcaa gtagttcaca caaaggaccg cggtatcacc ggcgatgccg aacacgtcca
     1021 catcgccgat gtccatgttc accaccacgc tcttcgcatg gtagcgttca aggcgttcca
     1081 tcttgacgcg caactcctcg gcctcctcga actccagcgc ctccgcgtgg cggagcatct
     1141 gtgctttcat gccgcgcatg agaccactca tacggccttt caccaattgg cgcacctcgg
     1201 ccatgtccgc gttgtactcc tcctcggatc gcaatccctc gcagggggct ttgcagttgc
     1261 cgatgtggta ctcgagacag cgcttgaact tccccttcgc gatgttggcc ggggacaggt
     1321 cgtagttgca ggtgcgcaac ttgtacaggc catgcaccag cctgagcatc gtgttcatgc
     1381 ccaagcccga tgcgaagggg ccgtagtact cactaccgtc ctccaccggg ttgcgcatgc
     1441 cctccacgcg ggggaaatgc tcattgcgaa tacggatcag cgggtagctc ttgtcgtcgc
     1501 gcaacaggat attgtagcgg ggctgatgtt ccttgatgag cgtgttctcc agcagcaggg
     1561 cttcgaactc gctgggcacc ac
//
LOCUS       DAOQOC010000825         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61659_length_1582_cov_3.447282, whole genome shotgun sequence.
ACCESSION   DAOQOC010000825 DAOQOC010000000
VERSION     DAOQOC010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61659_length_1582_cov_3.447282"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1582)
                     /locus_tag="PL106_12150"
     CDS             complement(<1..>1582)
                     /locus_tag="PL106_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HNA33867.1"
                     /translation="QTREQHIRRDKATSNICTAQALLAVMAGMYAVYHGPDGLKRIAR
                     NVHAHTRNVAEAARALGYSVANSGYFDTITLSGVQDVKKVRAAAEKRGINFRYDGNNV
                     GIALDETVRAGDVVDVVASLAEAIGVKAPAAIGNGATMCVPAGLQRTSAFLTHPIFNT
                     YHTELELQRYIKRLENKDFSLMHGMIPLGSCTMKLNAASTLIPLSWPEWAGLHPFAPV
                     DQAQGYQDLIRQLSDALAKITGFTAVSLQPNSGAQGEYTGLLVIRAYHEAQGDHQRNV
                     ALIPSSAHGTNPASAAMVGMNIVVVKADAEGHVDMADLKAKAEQYKDTLSCLMITYPS
                     THGVYEETIQEITGTIHANGGLVYMDGANMNAQVGLTSPGGIGADVCHLNLHKTFAIP
                     HGGGGPGMGPICVNDKLKPFLPGHPVITTGGEQAVHAVSAAPWGSSLILLISYGYIKM
                     LGSDGLTDATRYAILNANYLRAGLEKHYPILYTGTEGMVAHEMILDCRDFKRAAGIDV
                     SDIAKRLMDYGFHAPTVS"
BASE COUNT          240 a          492 c          525 g          325 t
ORIGIN      
        1 aaactcacgg tgggcgcatg gaagccatag tccatcaggc gcttcgcgat gtcgctcacg
       61 tcgatgccgg ccgcccgttt gaaatcgcgg caatcgagga tcatttcatg cgccaccatg
      121 ccttcggtgc ccgtgtacag gatggggtag tgcttctcca gtccggcgcg caggtagttg
      181 gcgttgagga tggcgtaccg cgtggcgtcc gtcagtccat cgctgccgag cattttgatg
      241 taaccgtagc tgatgagcag gatgagcgaa ctgccccagg gcgcggcgct cacggcatgc
      301 acggcctgct caccaccggt ggtgatcacc ggatggccgg gcaggaaggg cttgagcttg
      361 tcgttcacgc agatggggcc catgcctggg ccgccgccac cgtgcgggat ggcgaaggtc
      421 ttgtgcaggt tgaggtggca cacgtccgct ccgatcccgc ccggactggt caggcccacc
      481 tgcgcgttca tgttcgcgcc gtccatgtac accaggccgc cgttggcgtg gatggtgccg
      541 gtgatctcct ggatcgtctc ctcatacacg ccgtgggtgc tcgggtaggt gatcatcagg
      601 cagctcagcg tgtccttgta ctgttcggcc ttggctttca ggtcggccat gtccacatgc
      661 ccttccgcat cggccttcac caccacgatg ttcatgccca ccatggccgc gctggcgggg
      721 ttggtgccgt gcgcgctgct ggggatgagc gcgacgttgc gctggtggtc cccctgcgcc
      781 tcgtggtagg cgcgtatgac cagaagtccg gtatattcac cctgggcacc gctgttcggt
      841 tgcaggctca cggccgtgaa gccggtgatc ttcgccagcg catcgctgag ctgacggatg
      901 agatcctggt acccctgggc ctgatccacc ggagcgaagg gatggagccc ggcccactcg
      961 ggccacgaca gggggatcag ggtgctcgcc gcgttcagct tcatggtgca cgaacccagt
     1021 ggtatcatgc cgtgcatcag ggagaaatcc ttgttctcga gccgtttgat gtaccgctgc
     1081 aactccagtt cggtgtggta cgtgttgaag atcgggtgcg tgaggaaggc gctggtgcgc
     1141 tgcagaccgg cgggtacgca catggtcgcc ccgttgccga tcgcggcggg ggccttcacg
     1201 ccgatcgcct cggccagcga tgcgaccaca tcgaccacat caccggcacg cacggtctcg
     1261 tcgagggcga tgcccacgtt gttcccgtcg taacggaaat tgataccgcg cttctccgcg
     1321 gctgcccgta ccttcttcac atcctgcacg ccggagagcg tgatcgtgtc gaaatagcca
     1381 ctgttggcca cggaatatcc caacgccctg gcggcttctg ccacgttgcg ggtgtgggca
     1441 tgcacgttgc gggcgatgcg cttgagcccg tcgggcccgt ggtacaccgc gtacatgccg
     1501 gccatcacgg ccaacagggc ctgcgccgtg cagatgttgc tggtggcctt gtcgcggcgg
     1561 atgtgctgct cgcgtgtttg ca
//
LOCUS       DAOQOC010000826         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61888_length_1579_cov_3.799869, whole genome shotgun sequence.
ACCESSION   DAOQOC010000826 DAOQOC010000000
VERSION     DAOQOC010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61888_length_1579_cov_3.799869"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PL106_12155"
     CDS             complement(<1..1113)
                     /locus_tag="PL106_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNA33868.1"
                     /translation="MMEVEVDMPILAPFQDEWVRDGSRFRVIEGATGTGKTYVFEPEL
                     FAEAHKEDANGYEFWWCSPTIAQARAVYENVKRSIEDAGLSTLYRCSDTLREIHTPGG
                     GVLCYRTAEHPDHLFGIRNVKHIIVDEFTRCRIALWPALLSVANKTGARITFIGNYQG
                     DDTEWHAWAKKMATTPKFRYWKTPAGEAVKAGIMAQEDFNMARETLPDPIFKALYLCE
                     GSSDPNLLVQRSAVADLWTNSHVIEGPPALTCDIALHGSDRFVVMAWRGMVMVEVCVW
                     TKRSATEVEDIIRQKAKEHGAGLSQISYDADGLGTYLRGYLKGGVPYTGGSAPVPEKG
                     KKMSYQRLRDQCHFLTADTINARGIWLKDDAHREELE"
     gene            complement(1110..1307)
                     /locus_tag="PL106_12160"
     CDS             complement(1110..1307)
                     /locus_tag="PL106_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33869.1"
                     /translation="MITKELALMEHSDNGGRVVKGSEQIEYYLKLGWYIIDNPRNRLL
                     PHRVVVRPDVQPEKKRRKRGE"
     gene            complement(1324..>1579)
                     /locus_tag="PL106_12165"
     CDS             complement(1324..>1579)
                     /locus_tag="PL106_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33870.1"
                     /translation="ILRKSVGLPPVNGGDAIPAVSERRMALCRACPVFNASTSSCGTL
                     ILDALTPSVVDVDGVPVQPCGCFLPLKVKLRRAICPAKKW"
BASE COUNT          268 a          507 c          470 g          334 t
ORIGIN      
        1 ctccaactcc tcgcggtgcg catcgtcctt cagccatatt cctcgcgcgt tgatcgtgtc
       61 ggcggtaagg aagtggcatt gatcgcgcag ccgttgatag ctcatcttct ttcccttttc
      121 cggcacgggt gctgacccgc ccgtgtaggg gacccccccc ttgaggtagc cgcgaaggta
      181 ggtgcccagg ccgtccgcat cgtagcttat ctgtgaaagg ccagcgccgt gttccttggc
      241 cttctggcga atgatatcct ccacctccgt agcggatctc ttcgtccata cgcacacctc
      301 gaccatcacc atgccgcgcc atgccatcac cacgaagcgg tcggagccgt gaagggcgat
      361 gtcgcacgtc agcgccgggg ggccttcgat cacatggctg ttcgtccaca ggtcagccac
      421 cgcgctgcgt tgaaccagaa ggttcgggtc ggatgaccct tcgcagaggt agagggcttt
      481 gaagatcgga tcgggcagcg tttcgcgcgc catgttgaag tcctcctgcg ccatgatccc
      541 ggccttgacc gcttcccctg ctggcgtctt ccagtacctg aatttcgggg tcgtggccat
      601 cttcttcgcc cacgcgtgcc attcggtatc gtccccctga tagttgccga tgaaggtgat
      661 gcgtgcgccg gtcttgttcg ccacagacag cagcgcaggc catagggcga tgcggcagcg
      721 cgtgaactcg tcaacgatta tgtgcttcac gttgcggata ccgaacaggt gatcggggtg
      781 ctccgccgtc ctgtagcaca ggacaccgcc gccaggcgtg tggatctccc tcaaggtatc
      841 gctgcaccgg tacagcgtcg aaaggcccgc atcctcaatg gaccgcttca cgttctcgta
      901 gaccgcgcgc gcctgtgcga tggtcggtga gcaccaccag aattcgtaac cgttggcgtc
      961 ctccttgtgc gcctccgcga atagctccgg ctcgaacacg taggtcttgc ccgttccggt
     1021 agctccttcg atcacgcgga agcgtgatcc gtcccttacc cattcatcct ggaatggcgc
     1081 aaggatcggc atatcgacct ccacctccat cattcaccgc gcttgcggcg cttcttctcg
     1141 ggttgcacat ccgggcgcac caccacgcgg tgcggcaata ggcggttgcg ggggttgtcg
     1201 attatgtacc acccgagttt aaggtagtat tcgatctgct cgctgccctt gaccacacgt
     1261 ccgccgttgt cggagtgctc catcagcgcc agctccttcg ttatcatcct ggggtacgcc
     1321 atgtcaccac tttttggccg ggcagatggc ccgacggagt ttcactttca agggcaggaa
     1381 gcagccgcac ggctgcacgg gtacgccgtc cacatccacc accgatggcg tgagggcgtc
     1441 aaggatcagc gtgccgcagg aagatgtgga cgcgttgaag acggggcagg cgcggcataa
     1501 ggccatgcgc cgctcggaca cggcggggat ggcatcgcca ccatttaccg ggggcaggcc
     1561 gacagacttg cgcaggatg
//
LOCUS       DAOQOC010000827         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61899_length_1579_cov_3.398950, whole genome shotgun sequence.
ACCESSION   DAOQOC010000827 DAOQOC010000000
VERSION     DAOQOC010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61899_length_1579_cov_3.398950"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..1207
                     /gene="thyX"
                     /locus_tag="PL106_12170"
     CDS             509..1207
                     /gene="thyX"
                     /locus_tag="PL106_12170"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNA33871.1"
                     /translation="MDANRSEGRKVNFLPDGKVTLVDTMGSDLTVVNAARVSFNKHVD
                     TVGEADRGLIRFLIENRHGTPFEHVAFTFHVHCPIFVAREWQRHRIGSFSEVSGRYSE
                     LEPVFWLPEQLRRQVGKAGAYTFEDITDGFVHAEGRNDISFAYENAWASYQDLIEMGV
                     AKEVARAVLPVGTFTQFYWSVNLRSLFNFLSLRNAPQAQREIRVLAQQVEDIVSAHMP
                     ETMSTWRTAGGTAV"
     gene            1204..1539
                     /locus_tag="PL106_12175"
     CDS             1204..1539
                     /locus_tag="PL106_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33872.1"
                     /translation="MSAIFQHGEGSVSDADQRWQDALARARRQDVLAEVGRERSRQEQ
                     LKAKGKFTYTCADAEMTDGQRLAVLVEEVGEVARAMNDGKGLREELIQVAAVAVAWVE
                     AIDTAEGAG"
BASE COUNT          315 a          483 c          517 g          264 t
ORIGIN      
        1 cccagaacaa gctcttccac gtcttcggcg tctacgaagg agaaagcatt ggctgagcac
       61 aagcccaact gcctgcactg ctggtgtcgc cttttcgagt ccaagcggat gtgctgcgag
      121 tgcggcgcgg tggcaccgag gacggaggac cgatgagcga ctggtgcgag gcggacgtgc
      181 tattgcacgg taatgccgtg cccgtcttcg tctttggcgt gggtacctat aaagacaccg
      241 gatgcgagca tcacccggcc tgcctctcgt gtccgttcga aaagtgcgtc ctcgaaggtg
      301 gtgcagcgcc accacttgcc gtggaagtat cacctccgcc cgtgtgtcgc tactgcgggg
      361 tggagtttgt gcgacagaag actaaccacc gcccggccaa ctattgcgcc gaccaccagg
      421 gccaaccgtt ccggcaagag gttcgcgccc gctacaacgc aacacgaaag cgccagaagc
      481 gagaaaggga aaagaagatg gtggcaacat ggacgccaat cgatccgagg gccgcaaagt
      541 gaacttcctg cccgacggca aggtcaccct ggtggacacg atgggctcgg accttacggt
      601 cgtgaacgcc gctcgcgtgt ccttcaataa gcacgtcgat acggtgggcg aagccgaccg
      661 tggcctcatc cggttcctca tcgagaaccg ccacggcaca cccttcgagc atgtggcctt
      721 cacgttccac gtccactgcc cgatcttcgt tgcccgtgag tggcaacgac accgcatcgg
      781 ctcgttcagc gaggtgagcg gacgctactc cgaactggag cccgtcttct ggctgccaga
      841 gcaattgcgc cgccaggtcg gcaaggccgg ggcctacacc ttcgaggaca ttacagacgg
      901 attcgtgcat gccgagggtc ggaatgacat ttccttcgcc tacgagaatg cgtgggcttc
      961 ctaccaggat ctcatcgaga tgggcgtagc caaggaggtt gcccgggcgg tcctgccggt
     1021 cggcaccttc acgcaattct actggtccgt gaaccttcgc tccctcttca acttcctctc
     1081 cctgcggaac gccccgcagg cgcagcggga gatccgcgtc ctcgcccagc aggttgagga
     1141 catcgtgagc gcacacatgc ccgagaccat gagcacctgg cgcactgccg ggggtaccgc
     1201 cgtatgagcg ccatctttca gcatggggag ggcagcgtga gcgatgcaga tcagagatgg
     1261 caggatgccc tcgcgagagc gcgaaggcag gacgtgcttg cggaagtagg tcgcgagcgc
     1321 agccgccaag aacagctcaa ggcgaagggg aagttcacgt acacctgcgc cgatgcggag
     1381 atgacggacg gccagcggct cgcggtactc gtggaggaag tgggcgaggt ggcacgggcc
     1441 atgaacgacg gcaagggcct gcgcgaggag ctgatccagg tcgcggcggt ggccgtggcg
     1501 tgggtcgagg ccatcgacac ggcggagggt gcgggatgat ctgcccgaac agcggccacg
     1561 caacgtaccg gacgcgcgt
//
LOCUS       DAOQOC010000828         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61902_length_1579_cov_3.298556, whole genome shotgun sequence.
ACCESSION   DAOQOC010000828 DAOQOC010000000
VERSION     DAOQOC010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61902_length_1579_cov_3.298556"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="mutS"
                     /locus_tag="PL106_12180"
     CDS             complement(<1..1422)
                     /gene="mutS"
                     /locus_tag="PL106_12180"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNA33873.1"
                     /translation="MEGPSRPAGSRGPPVARIHPRELLHLGRSIEAAERTRQDLLTAD
                     ERLARMAGDWAPPQKLARAISDHIEPEAPVNIAKGGVLKPGVDSELDDLRHVTSHAKD
                     LLMGIQQREADRTGITSLKVGYNNVFGYYLEVRHTHREKVPADWTRKQTLTGAERYIT
                     AELKELEERILSAEERIGSVEQRLFTGLVEQVRAATPDLQATAAALAALDVLTSFAEN
                     AVLWRYARPELTNDPRLEVREGRHPVIEQQLPPGEPYVTNDLLLDPQDRQLVVITGPN
                     MSGKSALLRQTALHVLMAQCGSFIPARSAVIGITDRVFTRVGASDDLSTGESTFMVEM
                     NETASILHNLTDRSLVLLDEIGRGTSTYDGISIAWAIAEYLHEHPGRPKTLFATHYHE
                     LNEMAGSFPRIHNANVSVREVQGKVLFLRKLVPGGTDRSFGIHVARMAGMPAPVIDRA
                     QKVLHHLELSHAGDLGEADGTPLS"
BASE COUNT          228 a          529 c          524 g          298 t
ORIGIN      
        1 cgagaggggt gtgccatccg cctcgcccag gtcaccggca tgggaaagct ccaggtggtg
       61 gagcaccttt tgggcgcggt ctatcacggg cgcgggcatg ccggccatcc gggccacatg
      121 gatgccgaaa ctgcgatcgg ttccacctgg caccagtttg cgcaggaaaa gcactttgcc
      181 ctgcacttcg cgtaccgaca cgttcgcgtt gtggatgcgc gggaagctgc cggccatctc
      241 gttcaactcg tggtaatgcg ttgcgaaaag tgtttttggg cgaccgggat gctcatgcag
      301 gtactccgcg atggcccagg cgatgctgat gccatcgtag gtgctggtgc cgcgcccgat
      361 ctcgtccagc aacaccaggc tgcggtcggt caggttgtgc aggatgctcg ccgtctcgtt
      421 catctccacc atgaacgtgc tttctccggt ggagaggtcg tcggaagcgc ccacacgggt
      481 gaacacgcga tcggtgatgc cgatcacggc gctgcgagcg gggatgaagc tgccgcactg
      541 ggccatcagc acgtgcaggg cggtctggcg cagcaaggcg ctcttcccgc tcatgttggg
      601 gccggtgatc accaccagtt gccgatcctg aggatccagt agcaggtcgt tcgtgacgta
      661 cggctcgccc gggggcaatt gctgttcgat caccgggtgg cggccctcgc gcacctccaa
      721 ccgcggatca ttggtgagtt cggggcgggc ataacgccac aacaccgcgt tctcggcgaa
      781 actggtcagc acatccagcg cggccagtgc ggcggccgtg gcctgcaggt cgggggtggc
      841 cgcgcgcacc tgctccacca gtccggtgaa gagccgctgt tccaccgagc cgatccgctc
      901 ctcggcgctc aggatccgtt cctccagttc cttcagctcc gccgtgatgt accgttcggc
      961 cccggtgagc gtctgcttgc gtgtccagtc ggcgggtact ttttcgcggt gcgtatggcg
     1021 cacttccaag tagtagccga acacgttgtt gtaccccacc ttcagcgagg tgatgccggt
     1081 gcgatccgcc tcccgctgct ggatgcccat gagcaggtcc ttggcatgcg aggtgacatg
     1141 ccgcaagtcg tccagttcac tgtccacgcc gggtttcagc acgccaccct tggcgatgtt
     1201 caccggggcc tccggttcga tgtggtcgct gatcgcgcgt gccagcttct ggggcggagc
     1261 ccagtccccg gccatacggg ccaggcgttc gtcggcggtc agcaggtcct gccgggtgcg
     1321 ttcggccgcc tcgatggagc gccccagatg cagcagttcg cgcgggtgga tccgcgcgac
     1381 cggtggcccg cggctcccgg caggtcgtga cggtccctcc aacgcgcttg agtcgctccc
     1441 gcacccggcc cgcgccctgc atcgctgcga agcggggtgc tgacgcaccg tgttctgacc
     1501 ctcgcacccc cccacccccg gcttggtccg cgcagtcgtc cgctgccggc ccgcgaaacc
     1561 cgggacagga gtgccaggc
//
LOCUS       DAOQOC010000829         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61907_length_1579_cov_3.159449, whole genome shotgun sequence.
ACCESSION   DAOQOC010000829 DAOQOC010000000
VERSION     DAOQOC010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61907_length_1579_cov_3.159449"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PL106_12185"
     CDS             <1..1229
                     /locus_tag="PL106_12185"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNA33874.1"
                     /translation="YDADLGKLYFSSDFLPGFGGYDNFMSRDSAGSFTAPLNFMYPLN
                     SPANDLYPTFDLKTRTGYFTSNRVGSLAKKGATCCNDIYRYHYPALRLAQRDSTGTVR
                     AQDSLPGEAPTVAEKRITSLREKLPIRLYFHNDEPGPRSRDTLTALTYEATWRAYRAL
                     RPDYHRAWQGDTTAIEAFFRDNVDAGLARLNEFTTLLRQALDEGQRIELRVRGFASPL
                     AESDYNARLSLRRISSLVNQLRTTLGGALRPFLESGALRLVKSPFGEDRSATGVSDRL
                     DDLQGSVYSVGAALERRIEIEQVMDAGAGAQSLSAPLTLDMGPLLQGMERTDTIHVRN
                     DTGTPVTLHGAQLDCDCFRVQVPDEPIAPGTIVGIHVTFTGRLRPGHVSREAIIPTSG
                     GPAILRLTITGTVIPH"
     gene            1233..>1579
                     /locus_tag="PL106_12190"
     CDS             1233..>1579
                     /locus_tag="PL106_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNA33875.1"
                     /translation="MKLLLLSNSTMPGEPYLGWPREHIRAFLGEPKRIAFVPFAAVTY
                     PLDEYARITTEAFAQLGHACFSLHSETDKVKALSAADAVAVGGGNSFQLLRTLYSAEL
                     IRALRVKVMGGMP"
BASE COUNT          306 a          538 c          469 g          266 t
ORIGIN      
        1 actatgacgc cgacctggga aaattgtact tcagcagcga cttcctgccc ggtttcggtg
       61 gctacgacaa cttcatgagc cgggacagtg cgggcagttt caccgcgccg ctgaacttca
      121 tgtacccctt gaacagtccg gcgaacgacc tctaccccac attcgacctg aagacccgga
      181 cagggtactt caccagcaac cgcgtcggct cactggcaaa aaagggggcc acctgctgca
      241 atgatatcta ccggtatcat tatccagcgc ttcggctcgc tcagcgtgac agcacaggga
      301 cggtgagagc tcaggacagc cttcccggtg aagcgcccac cgtggccgag aagcgcatca
      361 cctcactgcg ggaaaagctg cccatccgcc tgtacttcca caacgacgag cccgggcctc
      421 gctcccggga cacgctcacc gcgctcacct acgaggccac ctggcgggcc tacagagcgc
      481 tgcgccccga ttaccatcgc gcctggcagg gcgacaccac cgccattgag gccttcttcc
      541 gcgataacgt ggacgcgggc ctcgcccgtt tgaacgaatt cacaaccctg ctgcggcagg
      601 cactggacga agggcaacgc atagaactcc gggtgcgcgg ttttgccagc ccgctggctg
      661 aaagcgacta caacgcccgg ctctcgctgc ggcgtatcag cagcctggtg aaccaattac
      721 gcacgacact gggcggggcg ctccgaccgt tcctggaaag cggggccctc cgcctggtga
      781 agtcgccctt cggcgaggac cgcagcgcaa caggtgtgag cgatcgcctg gacgacctgc
      841 aaggctccgt gtacagcgtg ggtgctgcgc tggaacgccg catcgagatc gagcaggtga
      901 tggatgccgg tgcaggagca cagtccttga gcgcaccatt gacactggac atggggcccc
      961 tgttgcaggg tatggaacga acggatacga tacacgtgag gaacgatacc ggtacgcctg
     1021 tcacgctcca cggcgcacag ctcgattgcg actgcttccg ggtacaggtg cccgacgaac
     1081 cgatcgcccc cggtacaatt gtcggtatcc acgtgacctt caccggtcgc ctgcgccccg
     1141 ggcacgtgag ccgcgaggcg atcatcccca ccagcggtgg accggctatc ttgcggctga
     1201 cgatcaccgg caccgtgata ccgcattgaa caatgaagct cctcctgctt tccaactcca
     1261 ccatgccggg cgagccctac ctcggctggc cgcgcgaaca catccgcgct ttcctgggtg
     1321 aaccgaaacg catcgccttc gtgcccttcg ccgccgtgac ctatccgctg gacgagtacg
     1381 cgcgcatcac cacggaggcc ttcgcccaac tggggcacgc gtgcttcagc ctgcattccg
     1441 aaacggacaa ggtgaaggcg ctctccgcgg ccgacgccgt ggccgtgggc ggcggcaact
     1501 ccttccagct gctgcgaacg ctctacagcg ctgaactgat acgtgccctc cgggtgaagg
     1561 tgatgggcgg catgccgtt
//
LOCUS       DAOQOC010000830         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_61911_length_1579_cov_3.031496, whole genome shotgun sequence.
ACCESSION   DAOQOC010000830 DAOQOC010000000
VERSION     DAOQOC010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61911_length_1579_cov_3.031496"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1215
                     /locus_tag="PL106_12195"
     CDS             109..1215
                     /locus_tag="PL106_12195"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNA33876.1"
                     /translation="MLHAPRYGRRRSTAPGRDATHAVRDAFQRSYFHPEVHSVRMQQL
                     IDAFPQQLKEALAIGRSAKLSAPSGPYTNVVVTGLGGSGIGGRIAAQVVAAEANCPIE
                     VYSNYYLPAYVNHNSLVIACSYSGNTEETIASMEQALAKGARVCVVTSGGAMLEMAQK
                     RGLDHIVIPGGNPPRTMLAYSLTQQFFVLHHFGIIGDGFEGAIKAAAAMLEEDKAKIQ
                     SAAKDLAEKLFGKRLVIYSEASTEAVSIRFRQQVNENSKELCWHHAIPEMNHNELVGW
                     AGGKDDIAVVIFRHKTDHERSQIRMGINKEVFCRHTPHIHDVWSQGDTTIARQLYLIN
                     LGDWVSFYWAEKKGVDPSEIAVINMLKGKLAEVK"
     assembly_gap    1225..1250
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1295..>1579
                     /locus_tag="PL106_12200"
     CDS             1295..>1579
                     /locus_tag="PL106_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase"
                     /protein_id="HNA33877.1"
                     /translation="MRSVHFRDLGLIDYAEAWDMQTRLFQATIDRKLANRNLPTEDQQ
                     ATEDHLLFCEHPHVYTLGKSGMQSHLLLNEEGLRAKSVQFFPIDRGGDITY"
BASE COUNT          340 a          502 c          443 g          268 t
ORIGIN      
        1 ttctttgacc aacgtcagca cttgccgatc gcctgccctc tcggccccga acagacctca
       61 cgtataccgc tcccagaccg gccattaagg gccagggcct tccggcgcat gctgcacgcc
      121 ccccggtacg gccgcaggcg cagcacggct cccggccgtg acgcaaccca tgccgttcgc
      181 gatgcgttcc aacgctccta ttttcacccc gaagtccatt ctgtacgcat gcaacagctc
      241 attgatgcct ttccgcagca actgaaagaa gccctggcga tcggccgctc ggccaaactg
      301 agcgcaccaa gcgggcccta caccaacgtg gtggtcaccg ggctcggcgg cagcggcatc
      361 ggtggtcgta tcgccgctca agtggtggcc gctgaggcca actgccccat tgaggtgtac
      421 agcaactatt acctcccggc gtatgtgaac cataatagct tggtgatcgc ctgcagctac
      481 agcggcaaca cggaggagac catcgcttcc atggaacagg cgcttgccaa aggcgcgcgg
      541 gtctgcgtgg tcaccagtgg tggagcgatg ctggaaatgg cccagaagcg cgggctggat
      601 cacatcgtga tccccggcgg caacccgccc cgcaccatgc tggcgtattc gctcacgcaa
      661 caattcttcg tgctccatca cttcggcatc atcggcgacg ggttcgaagg cgcgatcaag
      721 gccgccgccg ccatgctcga ggaggacaag gcaaagatcc agtccgccgc caaggacctt
      781 gcggagaagc tcttcggcaa gcgcctggtg atctacagtg aggccagcac cgaagccgtg
      841 agcatccgct tccgccaaca agtgaacgag aacagcaagg aactctgctg gcaccacgcc
      901 attccggaaa tgaaccacaa cgaactcgtg ggctgggccg gcggaaagga cgacatcgcc
      961 gtggtgatct tccggcacaa aacggaccat gaacgctcgc agatccgcat ggggatcaac
     1021 aaggaggtgt tctgccgcca taccccgcac atccacgatg tgtggagcca gggcgatacc
     1081 accatcgcgc gtcagttgta cctgatcaac ctgggcgact gggtgagttt ctactgggcc
     1141 gaaaagaagg gcgtggatcc cagcgagatc gctgtgatca acatgctgaa gggaaagctc
     1201 gccgaagtaa agtgacgagt ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn ggaggcagca
     1261 ctcgccactc accactcgcc acttactacg cccgatgcgc tccgttcatt tccgcgacct
     1321 tggcctcatc gactatgctg aagcgtggga tatgcagacg cgcctgttcc aggcgacaat
     1381 tgaccgtaag ctggccaacc ggaacctgcc caccgaagat cagcaggcga cggaagacca
     1441 tctgctcttc tgtgagcacc cccacgtata caccctgggc aagagcggca tgcaaagtca
     1501 cctgcttctg aacgaggaag gtcttcgcgc taaaagcgtt cagttctttc ccatcgaccg
     1561 gggtggcgac atcacgtac
//
LOCUS       DAOQOC010000831         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62058_length_1577_cov_3.771353, whole genome shotgun sequence.
ACCESSION   DAOQOC010000831 DAOQOC010000000
VERSION     DAOQOC010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62058_length_1577_cov_3.771353"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1577)
                     /locus_tag="PL106_12205"
     CDS             complement(<1..>1577)
                     /locus_tag="PL106_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33878.1"
                     /translation="CVSLYGGGYFESLTPQAKGQAEAFPFAPDWTDMHDPAGSLAVTT
                     LPQPGGENTYEDQQATRSQMVRTDGNGCVLFFAVDGRLYDHAGYLIADARGDVHGDED
                     CQQCLEPGVMEMISIPVFGHCGLYYIFSARSGAEPGWSHDWPTRVQVALLDLNATNPW
                     HPDRKGALVYMYDGFPEDTFGITFDVDNQAADISSTYVRWLGWSFKGVSNTPMLRAID
                     PTGSGEMYWLYVIGTQAVVQYKVDASGVHLLADGMYQEHYQPVHSAWAGMNDKPYYRD
                     AAVTVNANNEVLLAMTDDELQGWSSGSFSSGHGPVLVLRFNATTGTFMGAEEIPYGSG
                     GLPDFGNDNGGSPPPPGPGLTGGPGGIAWVDSGTKLLIQGEAVVNNAWAHRVGIFDLA
                     TDQWTDLTSTLNVQDVQDHIYARISGNVESGNLAAYYLPHTAGVSRIGGLPNAPSWTA
                     SAPTSDPVALLPFEGYPDDDLYHPYFTNAQVVQDDAYLDGLNDPTCCAVYTAYTGVSG
                     HTFPNIPGTYHWYPDDN"
BASE COUNT          310 a          472 c          477 g          318 t
ORIGIN      
        1 gttgtcatcg gggtaccaat ggtaggtgcc ggggatgttg gggaaggtgt ggccggaaac
       61 acccgtgtat gcggtgtaca ccgcgcaaca ggtcgggtcg ttcaggccgt cgaggtacgc
      121 atcatcctgc accacctgtg cattggtgaa gtagggatgg tacaggtcat catccggata
      181 cccctcgaac ggaaggagcg ccactgggtc cgaggtgggt gcacttgccg tccagctcgg
      241 tgcgttggga aggccgccga tgcgcgagac tccggccgtg tgcggcagat agtatgccgc
      301 gaggttcccc gactcgacat tgccgctgat acgggcatag atatgatcct gaacgtcctg
      361 tacgttcaag gtcgaggtca ggtccgtcca ctggtccgtg gccaggtcga agatgcccac
      421 gcggtgcgcc caggcgttgt tcaccacagc ctccccttgg atgaggagtt tggtgccgct
      481 atccacccac gcaatgcccc cggggcctcc ggtaagtcct ggtcctgggg gtgggggcga
      541 tcccccgttg tcgttgccga agtcgggcag tccaccggaa ccgtacggga tctcctccgc
      601 ccccatgaac gttccggtgg tcgcattgaa ccgaaggacg agcaccgggc catggccgct
      661 gctgaagctg cccgaactcc agccctgaag ttcgtcgtcg gtcatggcca acagcacttc
      721 gttattggcg ttcaccgtaa cagcagcgtc gcggtagtac ggcttgtcat tcatgccggc
      781 ccaggcgctg tgcacaggtt ggtagtgttc ctggtacatg ccatcggcca gtagatgcac
      841 gccactggca tccactttgt attgtacaac ggcctgtgtc ccgatcacgt atagccagta
      901 catttcaccg ctccccgtag ggtcaatggc ccgtagcatg ggtgtgttgg aaacaccttt
      961 gaaggaccag cccagccaac gaacataagt ggacgaaata tcggccgctt ggttgtcaac
     1021 atcgaacgtg atgccgaagg tgtcctctgg aaagccatcg tacatgtaga ccagagcacc
     1081 cttgcgatcg ggatgccaag gattggtcgc attgaggtcc aagagcgcca cttgtacccg
     1141 cgtgggccaa tcgtgcgacc agcccggctc agcaccgctg cgggccgaga agatgtaata
     1201 tagaccgcag tggccgaata ctggaatgga tatcatctcc atcaccccgg gctccaagca
     1261 ttgttgacag tcttcatcac catggacatc accacgtgcg tcggcgatca agtagcctgc
     1321 atgatcgtac aagcgcccgt ccacagcgaa gaagagcacg catccattcc cgtccgtgcg
     1381 caccatttgc gaacgggtgg cttgctggtc ttcgtaggta ttctcccctc ctggttgtgg
     1441 caaggtcgtt acagcgagag accccgccgg gtcgtgcatg tcggtccagt cgggcgcgaa
     1501 aggaaacgcc tccgcctgcc ccttggcctg cggcgtgagc gattcaaaat accccccccc
     1561 atagagggat acacagc
//
LOCUS       DAOQOC010000832         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62061_length_1577_cov_3.610381, whole genome shotgun sequence.
ACCESSION   DAOQOC010000832 DAOQOC010000000
VERSION     DAOQOC010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62061_length_1577_cov_3.610381"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PL106_12210"
     CDS             complement(<1..439)
                     /locus_tag="PL106_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA33879.1"
                     /translation="MSTKEQRGPKAVLVITYWSSADALVRTYTLPYVRMMLRAMPPGS
                     RVYLVTLEKPGPTCQRIEEDGIIHLPFTYHALGLAGAAMIAGLTWRLTRLVRRYRIDT
                     VHAWCTPAGMVGYIVSVLTGRPLVIDSFEPHAEAMVENGTWRRS"
     gene            complement(436..>1577)
                     /locus_tag="PL106_12215"
     CDS             complement(436..>1577)
                     /locus_tag="PL106_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33880.1"
                     /translation="QYIYFDSRTWMVIRLLDPLVTIAFRSYLVTTVMLASAAYMGVWR
                     CFRMFVGYFPSQTGQLAMAFLFVPSVIFWGSGVGKDTFTFSGTCFFVYAVDQWFFKRN
                     RSFGTVLGGLLSASLVIYIKPYIFMALVPSMLLWVLYARISDIRNTLIKFVVVPFAFL
                     FLVAGTIAILSGLGDKLGKFSLDNAVSTAMVTNSDMTRNADYSANYFDIGPMDGTWSG
                     LLAKAPKAIFAALFRPRLDEARNPVMLISGLENAWLLWLAFSCLLRARIVLLPRFIFG
                     NPIVLMCLAFSVVFGFVIGISTPNFGALVRFKIPMLPFFITGLIVINYIVRLDRYYRE
                     RNRRLDLASLRQGTAHLRPEDDLPTKGRQRKRPRPLMPEPIAEAG"
BASE COUNT          354 a          458 c          494 g          271 t
ORIGIN      
        1 cggatcttct ccaggtgccg ttctccacca tcgcttcggc atggggttcg aaactatcga
       61 tcaccagcgg cctgccggtg agcacggaca cgatgtaccc gaccatgccg gccggcgtac
      121 accaggcatg caccgtatcg atgcgatacc gacggaccag gcgcgtcaat cgccaggtga
      181 gaccggcgat cattgcagct ccggccaggc ccaaggcgtg ataggtgaac ggcaggtgaa
      241 tgatcccatc ctcttcgata cgctggcagg tcggccccgg cttctccaag gtgaccaggt
      301 acacccggct tccagggggc atggcgcgaa gcatcatccg gacatacggc agggtatagg
      361 tccgcaccag cgcatcagca ctggaccagt aggtgatcac caggacggcc ttggggccgc
      421 gttgctcttt tgtgctcatc cggcttccgc gatcggctcc ggcatcaacg ggcgtggccg
      481 cttgcgctga cgccccttgg tgggcaggtc atcctcagga cgaaggtgag cggtgccttg
      541 ccgaagggag gccaggtcca accttctgtt gcgctcgcga tagtaccggt ccaggcgtac
      601 gatataattg atcacgatga ggccggtgat gaagaagggc agcatgggga tcttgaaccg
      661 tacgagagcc ccgaagttgg gcgtcgagat gccgatgacg aagccgaata ccacggagaa
      721 agcgaggcac atgagcacga tcgggttgcc gaagatgaat cgcgggagca gcacgatgcg
      781 cgcgcgcaag aggcagctga acgccaacca caacaaccag gcgttctcca atccgctgat
      841 gagcatcacg gggttacgag cctcatccag ccttggccgg aacaacgccg cgaagatggc
      901 cttcggagcc ttggcgagca gaccgctcca tgtcccgtcc attggtccga tgtcgaaata
      961 attggcgctg taatcggcat tgcgtgtcat atccgaattg gtgaccatgg ccgtggatac
     1021 cgcgttgtcc agggagaact tgcccagttt gtcgcccagg ccactgagta tggcgatggt
     1081 accggccacc aggaaaagga acgcgaaagg gaccaccacg aatttgatca gggtgttacg
     1141 gatgtccgag atgcgcgcat agagcaccca gaggagcatg ctggggacca gggccatgaa
     1201 gatgtacggc ttgatgtaga tcacgagtga tgcgctgagc agtccgccga gaacagtacc
     1261 aaaactcctg ttgcgtttga agaaccattg gtccacggcg tacacgaaga agcacgtacc
     1321 actgaaggtg aaggtgtcct tgcccacgcc gctcccccag aagatcaccg atggcacgaa
     1381 caggaaggcc atcgcgagct ggcccgtttg gctggggaaa tagcccacga acatgcggaa
     1441 gcaccgccat acccccatgt aggccgcgct cgccagcatc accgtggtca caaggtagct
     1501 gcggaaggcg atcgtgacca gcgggtccaa gaggcggatc accatccacg tgcgcgagtc
     1561 gaagtagatg tactggt
//
LOCUS       DAOQOC010000833         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62097_length_1577_cov_2.671485, whole genome shotgun sequence.
ACCESSION   DAOQOC010000833 DAOQOC010000000
VERSION     DAOQOC010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62097_length_1577_cov_2.671485"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1577
                     /locus_tag="PL106_12220"
     CDS             <1..>1577
                     /locus_tag="PL106_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33881.1"
                     /translation="VPVVTLTGSPLLGAATGEWTLVSGTGVIADPTSPMTEVTGVTVG
                     VSVFAWSVFNGPCANPNTTDQVTITVNDGSAQPASAGVDQELCSTSSGTSLDGNAPVA
                     PATGTWTQLQGPPATITAPTDPNTTVTSLQPGVNSFVWTIDNGACGNSTDIVTIEVFD
                     VNIPAVSAGPDQDMCTPMTSATLAGSGLIVPATGTWTVLTGPGTVVTPGSPGSTVQNL
                     GIGLNTLQWTVDNGPCPTGGSDLVNITVYDAATAGAVAGPDQELCTPAIGTTLAGNTP
                     AAPATGTWTVIQGAAQLVDPTNPTTAVTGLVVGTSVLVWTVGNGPCGMTTDTVRIVMF
                     DGTMPDADAGADQEFCTPFTQTDLDATAPISPATGTWSVVQGTGVFSDPNDPHAVVTG
                     AGTGENIYRWTVYNGPCANGNTNDLVSVLVYDGNNPVANAGPDQAICLPVTSTVLAGS
                     SVTFPATGTWTLVSGTGLLANVNDPNTGVTGLTVGVNVFVWTVDNGPCANGVTTDTVR
                     VLIYDQAMPTADAGPDQS"
BASE COUNT          307 a          512 c          502 g          256 t
ORIGIN      
        1 gttcccgtgg tcaccctcac gggcagcccg cttcttggtg cggcgaccgg tgaatggacc
       61 cttgtttccg gtacaggcgt cattgccgat ccgacctcac cgatgaccga ggtcaccggg
      121 gtcaccgtgg gggtcagtgt cttcgcctgg agcgtcttca atggcccttg tgcgaacccg
      181 aacacgaccg accaggtgac catcacggtg aacgatggca gcgcccaacc ggccagtgcg
      241 ggcgtcgatc aggaactgtg cagcacgagc tcgggcacca gcctcgatgg caatgctcct
      301 gtggccccgg ccaccgggac ctggacgcaa ctgcaggggc cacccgccac catcacggcg
      361 cccacggatc ccaatacaac ggtaaccagc ctgcagcccg gggtgaacag tttcgtctgg
      421 acgatcgata acggtgcgtg cggcaacagc accgatatcg tgaccatcga agtgttcgac
      481 gtgaacatcc ccgcggtttc ggcgggcccc gaccaggata tgtgcacccc catgaccagt
      541 gccactctgg cgggcagcgg cctgatcgta ccggccaccg gaacatggac cgtgctcacc
      601 gggccaggaa ccgtagttac ccctggctct ccgggcagca cggtgcagaa cctgggcatc
      661 gggttgaata cgctccaatg gacggtggac aacggccctt gtcccacagg tggatccgac
      721 ctcgtcaata tcacggtgta tgatgcggcc accgcggggg cggtcgcggg ccctgatcag
      781 gaactctgca cacctgccat aggcacgacc ctcgcgggga atacgccggc agcacctgcc
      841 acgggcacgt ggaccgttat ccagggcgca gcgcagctgg tggacccgac gaacccgacc
      901 actgcggtca ccggccttgt cgtgggcacg tccgtactgg tatggacagt gggcaacgga
      961 ccatgcggca tgaccacgga taccgtgcgg atcgtgatgt tcgatggaac catgcccgat
     1021 gctgatgcgg gcgccgacca ggagttctgc acacccttca cacaaacgga cctggatgct
     1081 acggcgccga tatccccagc aacgggtact tggagcgtgg tgcaggggac aggcgtgttc
     1141 agcgacccga acgaccccca tgccgtggta acaggggccg gcaccggaga aaatatctac
     1201 cgatggacgg tgtacaacgg accttgcgcg aacgggaaca cgaacgatct ggtgagcgtt
     1261 ctggtgtacg acgggaacaa tcccgtggcc aacgcgggcc ccgaccaggc catctgcctg
     1321 cccgtgacgt caacggttct cgcggggagt tccgtgacct tcccggcaac gggcacatgg
     1381 acactggtga gcggtaccgg cctgctggcc aacgtgaacg atcccaatac cggtgtgacc
     1441 ggcttgaccg tgggtgtgaa cgtgttcgtc tggacggtgg acaacggtcc ctgtgccaat
     1501 ggggtcacga ccgacaccgt gcgcgtactg atctacgacc aggccatgcc aacggccgat
     1561 gcgggccctg accagtc
//
LOCUS       DAOQOC010000834         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62316_length_1574_cov_3.967742, whole genome shotgun sequence.
ACCESSION   DAOQOC010000834 DAOQOC010000000
VERSION     DAOQOC010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62316_length_1574_cov_3.967742"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..431
                     /locus_tag="PL106_12225"
     CDS             162..431
                     /locus_tag="PL106_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33882.1"
                     /translation="MANELPPAVRAALWSLDTGRIDLDRHRQRIVTNILNLGTHEAVT
                     WLFKTYPRERIAEVVAYPRPGEWDRRSLNFWALVFDVEPRMTSRF"
     gene            484..1056
                     /locus_tag="PL106_12230"
     CDS             484..1056
                     /locus_tag="PL106_12230"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA33883.1"
                     /translation="MAICRDEGFYLAGGTALALQIGHRDSVDFDFFTAQVIDTARLFD
                     VLQAAFQAMPLVKVQDERNSLTVIADGAIKMSFMRYPYLLLRPLVRSEHLDLADITDI
                     GCMKLSAITGRGTLKDYVDLYFILRRITLNELLILCAQKLPQLDRALVLKALVFFDDL
                     DPEPIRYVKDHAVPFDEVKKLLRAQAKKAY"
     gene            complement(1156..>1574)
                     /locus_tag="PL106_12235"
     CDS             complement(1156..>1574)
                     /locus_tag="PL106_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33884.1"
                     /translation="SGTDLVTTGTGDFQWFLDGSPISGATDTLYTPIANGDYTVVLTV
                     SPDCIFTSPVYTLLNVGVEEFTVTGLYLITNPVNDVLRIVNDGATTPCAIVDAGGRTV
                     MQEQLFTGANNVDLSSLRPGLYLLRTAVGAVRFVKE"
BASE COUNT          318 a          478 c          469 g          309 t
ORIGIN      
        1 cacgaaacgc agctcgccat tcatcctgcg tagcgcgagg tacccgccca gagggagtaa
       61 cgaggcgcac gcgaccactc ttcgctgcaa gtatgcacgg agggaacgga gggcgcagga
      121 gcagaaacgg aatggtgatg cgcgacgtac cttgccgggg catggcaaat gaacttccgc
      181 ccgctgtacg cgccgcattg tggtcgttgg ataccggccg catcgacctt gaccggcatc
      241 ggcagcgcat cgtcacgaac attttgaacc tgggcaccca cgaggccgtc acctggctgt
      301 tcaagaccta tccgcgcgag cgtatcgccg aggtggtggc ctatccccgg cccggtgaat
      361 gggaccgacg ctccttgaac ttctgggcgc tggtgttcga tgtggaaccc cgaatgacca
      421 gccgtttctg aagccatgtg ctggtccatc ctcgatgagc agcgccgagc catcttgcca
      481 cgcttggcca tttgccgaga cgagggtttc tatctggccg gcggtaccgc gctcgcgcta
      541 cagatcggcc atcgcgacag tgtcgacttc gactttttca ccgcgcaggt gatcgacacg
      601 gcccgcttgt tcgatgtgct tcaagccgca ttccaggcca tgccgctggt gaaagtgcaa
      661 gacgagcgga acagcttgac ggtgatcgcg gacggcgcca tcaagatgag cttcatgcgc
      721 tatccctatc tgttgctccg tcctttggtc cgatcggaac atctcgacct cgcggatatc
      781 accgacatcg gttgcatgaa gctctctgcc atcaccggcc gtggcacgct gaaggactac
      841 gtggacctgt atttcatact gcgcaggatc acgctcaacg aattgctgat cctgtgcgcg
      901 cagaagctgc cgcagctgga ccgcgcgctg gtgttgaaag cgctcgtctt tttcgatgac
      961 ctggaccccg aaccgatccg ttatgtgaaa gaccatgcgg tcccgttcga cgaggtgaag
     1021 aagctgttgc gcgctcaggc gaagaaggcg tattgaagct cgcatccggg acaaagcgtt
     1081 cggtcaaggc catgcggttg aatgtgaaag ccgcgacctc cttccggaag tcgcggcctt
     1141 cgctcaataa ggatatcact ccttcacgaa acgtaccgca cccacggccg tgcgcaacag
     1201 atacaggcct ggccgcaagg acgaaagatc cacgttattc gcacccgtga acagttgctc
     1261 ttgcatcacc gtgcgcccgc ccgcatccac gatcgcgcat ggggtggtgg caccatcgtt
     1321 cacgatgcgc agcacatcgt tcaccgggtt ggtgatcaag tacagaccgg tcacggtgaa
     1381 ctcctccacg cccacattca gcagcgtgta caccggtgag gtgaagatgc agtccggtga
     1441 aacggtgagc actaccgtgt agtcgccgtt agcgatcggt gtgtagagcg tatccgtggc
     1501 accgctgatg ggcgaaccat cgaggaacca ctggaagtcg cccgtgcccg tggtaacgag
     1561 gtccgtgccg gact
//
LOCUS       DAOQOC010000835         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62317_length_1574_cov_3.894009, whole genome shotgun sequence.
ACCESSION   DAOQOC010000835 DAOQOC010000000
VERSION     DAOQOC010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62317_length_1574_cov_3.894009"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PL106_12240"
     CDS             complement(<1..352)
                     /locus_tag="PL106_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33885.1"
                     /translation="MSCGCGHNDCGCETVKGRRGPRGLQGPAGTSGQNAQLLYGLGVP
                     LTNPPVTTAPAQWMDTGDGGHYWMWSIAGQNWVDTGVQVQGDDGAPGTNGTNGINAFT
                     TTTAPFTMPNEGTFV"
     gene            complement(362..1006)
                     /locus_tag="PL106_12245"
     CDS             complement(362..1006)
                     /locus_tag="PL106_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33886.1"
                     /translation="MAASFEINACLKETCDGLAYVDSTGFFSVSNPWGYNAPQDSVPT
                     LTTEGVFGYTSYTLSIWPALQGGYDSDSDPLLVVDLLTRPHTIDPETGRVTWSFSWAD
                     LGLTDQVARSGWWFIRLDPVLWEGDVQAYDYSTDQSFALTGHVTKLVDDAMIAALRKH
                     GFKCGGCKCGGTPIDELYQRYRIIRDFAPCNGMDDQFTDGIDWLYSILPLCGCK"
     gene            1153..1425
                     /locus_tag="PL106_12250"
     CDS             1153..1425
                     /locus_tag="PL106_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33887.1"
                     /translation="MAVILSFDPQTKEVHMLDPDTGGSTPVSVTTASPRELTDTQILV
                     LDILRMMIVGHALSSIPEGDERRATATRILQEQDLSWESLVNFASK"
BASE COUNT          290 a          503 c          472 g          309 t
ORIGIN      
        1 ccacgaacgt gccctcattg ggcatcgtga agggtgccgt ggtcgtggtg aatgcgttga
       61 tcccgttggt gccgttggtg ccgggcgcgc cgtcatcacc ctgcacctgc accccggtgt
      121 ccacccaatt ctgcccggca atggaccaca tccagtaatg gccgccgtcg ccggtgtcca
      181 tccattgggc gggggccgtc gttacgggcg ggttggtcag cggcacgccc aggccgtaca
      241 gcagttgtgc gttctgcccc gacgtgccag cggggccctg caagccgcgc ggaccgcgac
      301 ggcccttcac cgtttcgcac ccgcagtcgt tatgtccgca tccgcaactc atggggtctg
      361 gtcatttaca cccgcaaagg gggaggattg agtacagcca gtcgatgcca tcggtgaact
      421 ggtcgtccat gccgttgcat ggcgcgaagt cccggatgat gcggtaccgc tggtacaact
      481 cgtcgattgg cgtaccaccg cacttgcagc ccccgcactt gaagccgtgc ttgcgcaggg
      541 cggcgatcat cgcgtcgtcc accagcttgg tcacgtgacc cgtcagggcg aaactctggt
      601 cggtggagta gtcgtatgcc tgcacgtcgc cctcccacag cacggggtca agccggatga
      661 accaccatcc cgagcgggcc acctgatcag tgaggccgag gtccgcccac gagaaggacc
      721 acgttacgcg gccggtctcc gggtcgatgg tgtgtggccg cgtcagcagg tccaccacca
      781 gcagcggatc actatcgctg tcatatcccc cttgcaatgc tggccagatg ctcagagtgt
      841 agctcgtgta gccgaacacc ccttcggtgg tcagggtagg cacgctgtcc tgtggcgcgt
      901 tatagcccca ggggttgctc actgagaaga agcccgtgga atcaacgtat gcgaggccgt
      961 cgcaggtctc ctttaggcag gcgtttatct cgaaggaggc ggccatcggc ggtgtttgtc
     1021 gggccgaagg tagtgcctat ttagaattgt tctaaataga gccaccatat tgcatttgcg
     1081 atacttggcg ttgatctttg gcgccaggat cgtgaaggta tcgtaatgcg atagcaccgc
     1141 tacattcgcc gcatggccgt catcctgtcc ttcgatccac aaaccaagga ggtccacatg
     1201 ctggatccgg acaccggagg atcaaccccc gtaagcgtga caacggcatc cccgcgcgaa
     1261 ctcaccgaca cccagatcct cgtgctcgat atcctgcgca tgatgatcgt gggccacgcc
     1321 ctcagcagca tccccgaggg ggatgagcgg cgcgccaccg ccacgcgcat cctccaagag
     1381 caggacctgt cgtgggaaag cctcgtgaac ttcgccagta agtagaattt ctgtgcgctc
     1441 accaacggcc atacggaccc tgcttgttgc ttgcggcaca agttgaacgg aacgaactgg
     1501 cgtcgaaggc cctgcggcaa cgcaaacggg actgaccctc actccacccc cttgtgctgc
     1561 ctcgcctttt cagc
//
LOCUS       DAOQOC010000836         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62387_length_1573_cov_4.623847, whole genome shotgun sequence.
ACCESSION   DAOQOC010000836 DAOQOC010000000
VERSION     DAOQOC010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62387_length_1573_cov_4.623847"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PL106_12255"
     CDS             <1..755
                     /locus_tag="PL106_12255"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNA33888.1"
                     /translation="MHALKSAYLHVASGMARNAVATGSELTGPMMRAGNFEQETERYR
                     ELEQDPIIGFEKEFLRWMLSDGAAAVLLEAAPRGPFSLRLEWIESRSFANELDVCMYS
                     GGDKDAEGRFQGWKNYVPQDLASRSIFTMKQDVKLLSRNIVPIGVRYLREVFERRKLD
                     PARVDHMLVHYSSMFFRDKLHDEMVGQGIGIPLDKWFVNLPQVGNVGSASIFLQLRDL
                     LAGGLVKKGQHVLLMVPESARFSYAFALLTAV"
     gene            826..1227
                     /locus_tag="PL106_12260"
     CDS             826..1227
                     /locus_tag="PL106_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33889.1"
                     /translation="MKKEEVPQDDANMLEGKFRVVKYALNEKGEYEKVPSLGWEPENT
                     ALEQAWDLIHEKVEAARQAVITGKLSPLAYHMEKNMMDAGLLGKHMGLPTRKVKRHLE
                     PTGFATLDAATLQRYADALQLTVAELQHVPR"
     gene            1224..>1573
                     /locus_tag="PL106_12265"
     CDS             1224..>1573
                     /locus_tag="PL106_12265"
                     /inference="COORDINATES: protein motif:HMM:NF025757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNA33890.1"
                     /translation="MSNSVVIPFEHQQSAHCETGVLSNLMRFHGMRVSEPMAFGIGSG
                     LFYSHMPFIKLNGIPVTSYRILPGHIFKRFSRNMGVEMRRIKFRDPREAMDELDRTLD
                     KGLPAGMLTSVFYLP"
BASE COUNT          340 a          482 c          472 g          279 t
ORIGIN      
        1 gtatgcatgc cctgaaatcc gcgtacctcc atgtggccag cggtatggca cgcaacgccg
       61 tggccacggg cagcgagctc accggtccca tgatgcgcgc gggcaacttc gagcaggaga
      121 ccgaacgcta tcgcgaactg gagcaggacc cgatcatcgg tttcgagaag gaattccttc
      181 gctggatgct ttctgatggt gccgccgcag tgctattgga agctgctcca cgcggcccgt
      241 ttagcctgcg cctggaatgg atcgagagcc ggtcattcgc gaacgaactc gacgtgtgca
      301 tgtactccgg aggcgacaag gacgccgaag gccggttcca aggatggaaa aactacgtac
      361 cccaggacct ggcctcccgg tccatcttca ccatgaagca ggacgtgaaa ctgctcagcc
      421 gcaacatcgt accgatcggc gtgcgctatt tgcgtgaggt cttcgagcgg cgcaagctgg
      481 acccggcacg cgtcgatcac atgctggtgc attattcctc catgttcttc cgcgataagc
      541 tgcacgacga aatggtgggg cagggcatcg gcataccgtt ggacaagtgg ttcgtgaacc
      601 tgccacaggt gggcaacgtg ggctcagcct ccattttcct gcaactgcgc gacctgctgg
      661 ccgggggcct cgtgaagaag ggccagcacg tgctgctcat ggtgccggag agcgcacggt
      721 tcagctacgc cttcgcgctg ctgacggccg tatgagaccc gctgccacgg ctatcttcgc
      781 gcttctaaaa taacctcggg cttcccgggg ccgcgctgca ccaacatgaa aaaggaggag
      841 gtccctcagg acgacgccaa catgctggaa ggcaagttcc gcgtggtgaa atacgccctg
      901 aacgaaaaag gcgaatacga aaaggtaccc agtttgggct gggagcccga gaacacggca
      961 ttggaacagg cctgggacct gatccacgaa aaggtggagg ccgcccgcca ggcggtgatc
     1021 accgggaaac tcagcccatt ggcctaccac atggagaaga acatgatgga tgcggggcta
     1081 ctgggcaaac acatgggcct gcccacccgc aaagtgaagc gccacctgga gccaacgggg
     1141 ttcgccacat tggatgccgc cacgttgcaa cgctacgccg atgccttgca actgacggtg
     1201 gccgaactcc agcacgtgcc gcgatgagca acagcgtggt catccccttc gaacaccagc
     1261 agagcgcaca ctgcgagacc ggcgtgctca gcaacctcat gcgcttccat ggaatgcgcg
     1321 tgtcggaacc catggccttc ggtatcggtt cagggctctt ctacagccac atgccgttca
     1381 tcaagctcaa tggcataccg gtaaccagtt atcgcatact gccgggccac atcttcaagc
     1441 gcttctcgcg caacatgggc gtggaaatgc gccggatcaa attccgcgat ccccgggagg
     1501 ccatggatga gctggaccgt acactggaca agggcctgcc cgccggcatg ctcaccagtg
     1561 tgttctacct gcc
//
LOCUS       DAOQOC010000837         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62560_length_1571_cov_3.332454, whole genome shotgun sequence.
ACCESSION   DAOQOC010000837 DAOQOC010000000
VERSION     DAOQOC010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62560_length_1571_cov_3.332454"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PL106_12270"
     CDS             complement(<1..1262)
                     /locus_tag="PL106_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNA33891.1"
                     /translation="MRILELKAMRGPNYWSVKRHHLIVMRLDIEDLEEKPTDRIPGFY
                     ERIQALLPSMQSHRCSEDHEGGFWERVRRGTWMGHVIEHIALEIQTLAGMDTGFGRTR
                     STRDHGVYNVVFSYIEEPVGMFAAKAAVRIAQALIDGTPYDLEADIQQMREIREESRL
                     GPSTGSIVAEAVSRGIPYLRLNGQSLVQLGHGIHQKRIRATITSKTSNIGVEIACDKE
                     ETKQLLESYEIPVPKGRVVRTEEGLEAAIESVGYPCVIKPIGGNHGRGATIGIKNMEE
                     AKAALAKAKEISRSVIVERYITGLDHRLLVINHRFICAAKRTPACVVGDGKSTIAQLA
                     DEVNKDPRRGFGHENVLTRIDIDDHTLKLLALKGLTPDSVPKKDETVYLKATANLSTG
                     GTADDVTEVVHPFNVFLAERISRIIDLD"
BASE COUNT          263 a          467 c          509 g          332 t
ORIGIN      
        1 atgtccaggt cgatgatgcg gctgatgcgc tcggcgagga agacgttgaa ggggtgtacg
       61 acttccgtca cgtcgtcggc ggtgccgcct gtgctgaggt tggcggtggc cttcaggtac
      121 acggtctcgt ccttcttggg cacgctgtcg ggcgtgagac ctttcagagc gagcaacttc
      181 aacgtgtgat cgtcgatgtc gatgcgcgtg agcacgttct cgtgcccgaa gccgcggcgc
      241 gggtccttgt tcacctcgtc ggcaagctgt gcgatcgtgc ttttcccatc acccactacg
      301 caggcgggag tacgtttcgc ggcacagatg aagcggtggt tgatcacgag cagccggtgg
      361 tccaacccgg tgatgtagcg ctccacgatc acgctgcggc tgatctcctt ggccttggcc
      421 agcgccgcct tggcctcttc catgttcttg atgccgatgg tggcaccgcg cccgtggtta
      481 ccgccgatgg gcttgatcac gcaggggtag cccacacttt cgatcgctgc ctccaggcct
      541 tcttcggtgc gcaccacgcg gcctttgggc acggggatct cgtagctctc cagcagctgc
      601 ttggtctcct ccttgtcgca ggcgatctcc acaccgatat tgctggtctt gctggtgatg
      661 gtggcacgga tgcgcttctg gtggatgccg tggccgagtt gaaccaggct ctgtccattc
      721 aagcgcaggt atgggatccc gcgtgaaacc gcttcagcca cgatgctgcc ggtgctggga
      781 ccgaggcgcg attcctcccg gatctcgcgc atctgttgga tatccgcctc caggtcgtac
      841 ggggtaccgt cgatcagcgc ttgcgcgatg cgcacggcgg ccttcgccgc gaacatgcct
      901 accggttctt cgatgtagct gaagacgaca ttgtacaccc catggtcgcg cgtgctgcgg
      961 gtgcggccga aacccgtgtc catgccggcc agggtctgga tctccaaggc gatgtgctcg
     1021 atcacgtgac ccatccatgt accacgccgc acccgctccc agaagccgcc ctcgtggtcc
     1081 tcgctgcatc gatggctctg catgctgggc agcaaggcct ggatacgctc ataaaaaccg
     1141 gggatcctgt cggtgggctt ctcctccagg tcctcgatat cgaggcgcat gacgatgagg
     1201 tgatgccgtt tgacggacca gtagttgggg ccacgcatgg ccttgagttc gaggatgcgc
     1261 atgggtatgg ccgtgacctg cggcgaagga tgatggtaag agggcttgga ggtccacctt
     1321 ggcacaagtg cttcgatggg cgaggcggaa gatagcgggg tgttcggatg tccggatgct
     1381 tccatggaca gaaaaacggc gggagacctg cccgaccatg gaaaaccctg ttgatgacaa
     1441 ggggtcctga acgggtatgg tcccgtgcgc ccccctactt tcgctcggct tccctgaaaa
     1501 ggggacacgg ggcggggggt aaacatcagg catgacaccc aaaggcaaac tcatcgccat
     1561 cggcggcaac g
//
LOCUS       DAOQOC010000838         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62653_length_1570_cov_3.051485, whole genome shotgun sequence.
ACCESSION   DAOQOC010000838 DAOQOC010000000
VERSION     DAOQOC010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62653_length_1570_cov_3.051485"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PL106_12275"
     CDS             complement(<1..790)
                     /locus_tag="PL106_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA33892.1"
                     /translation="MARILIPSDFSDNAKRAAEYAVSVVGPDHRFIVFNALSYVGLSD
                     PMVASVAVEVYGETEEQVLRWAEEFRSITGAKNVTGRVDSGSVATVVRNIARNEGADL
                     VVMGRSGAGGASLFGSNTTDVIRNTAVPVLAVPVNLPLKSPLRILLANDHNEVDEGNL
                     GMLRTIALYSSAEVLITHIAREKNAGRGDGNRAIFDRALKGVALTFQDHPGEDVPSAL
                     AEAAATHNADMIAVLHRHRDFLDRMFNPSVARKLALESVLPLLVL"
     gene            1275..>1570
                     /locus_tag="PL106_12280"
     CDS             1275..>1570
                     /locus_tag="PL106_12280"
                     /inference="COORDINATES: protein motif:HMM:NF017778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33893.1"
                     /translation="MRRGIRPGPMSASSMADPAPIVTIRELVRELLRQRDPADHGAVL
                     TRYAIPGQDLETYFRWNTRHYTRTCVIRNDAFELLVVCYEPGQRTSIHDYDSELA"
BASE COUNT          325 a          511 c          460 g          274 t
ORIGIN      
        1 caagcacgag cagtggaagg acactttcca aggcgagctt gcgtgccaca ctcggattga
       61 acatgcggtc caggaaatcc cgatgacgat gcagcaccgc gatcatgtcg gcgttatgcg
      121 tggccgcggc ttcggcaagg gccgagggca catcctcacc agggtgatcc tggaaagtga
      181 gtgccacacc cttcagtgcc cggtcgaaaa tggcgcggtt cccatcaccc cggccagcat
      241 ttttctcccg cgcgatgtgc gtaatgagca cctcggcgga agagtacagc gcgatggtcc
      301 gcaacatacc gaggttgccc tcatcaacct cgttatgatc attggcaagc aggatccgca
      361 aagggctctt cagcggcagg ttcacgggaa ccgccaatac gggcacggcc gtattccgga
      421 tcacatcggt cgtattgctg ccgaacaagg aggcgccgcc cgcaccactc cggcccatca
      481 ccaccagatc ggcaccctcg ttgcgcgcga tattgcgcac cacggtggct acggaccccg
      541 agtccacacg tcccgtcacg ttcttcgccc cggtgatgga ccggaattcc tccgcccagc
      601 gcagcacctg ttcttcggtt tcgccgtaca cctccacggc cacactggcc accatgggat
      661 cggaaaggcc cacataagaa agcgcattga agacgatgaa ccggtgatcc ggccccacta
      721 cgctcaccgc gtattccgcg gcgcgtttcg cattgtcact gaaatcgctg gggatgagga
      781 tgcgtgccat ggcaggaatg atcgtagaag ccacaaggta ggccggatcc ggctttgtcg
      841 atcctgacaa tggtcaacag gcccgggtcc gttcggtcaa gcagcgtggt caggatcccc
      901 gaacccggcg tatcaatgcc cgaaggcaag ccatatcccg agcggaacaa ttggccgatc
      961 accccgggat ccgaggggcc cgtgatcaca cgatgggcca tcgaagcgct ggtcatcccg
     1021 cattcccatc ggtggtgacg acggccaatg ggcccgggcg caccgatgac cggacgcgct
     1081 cggtgaggag caacagcatg cttcatcaag gtgctcgcgc gggcaatggg gccggggcct
     1141 gccatactgc ggtgatccca catgatgata ccggtgcgaa gtgggaggta tatcatccac
     1201 gtgcatgaca tccgtcatcc gcaccacatc cggcagcggg taggtttgct cgccggaaca
     1261 cctcgaacgc acccatgcga cgcggcatcc gcccaggtcc catgtccgca tcctcgatgg
     1321 ccgacccggc ccccatcgtc accatccgcg agttggtgcg tgagttactg cgtcagcgtg
     1381 atcccgctga tcatggcgcg gtactcacgc gatacgccat ccccggtcag gacctggaaa
     1441 cctacttccg ctggaacacg cgccattaca cgcgcacgtg cgtgatacgc aacgacgcgt
     1501 tcgagttgct ggtggtatgt tacgaacccg ggcagcgtac gagcatccac gactacgaca
     1561 gtgaactggc
//
LOCUS       DAOQOC010000839         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62709_length_1569_cov_4.558785, whole genome shotgun sequence.
ACCESSION   DAOQOC010000839 DAOQOC010000000
VERSION     DAOQOC010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62709_length_1569_cov_4.558785"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PL106_12285"
     CDS             <1..544
                     /locus_tag="PL106_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA33894.1"
                     /translation="TGNLPVVHSEIEALAAHLPVMRLNMEVPVALTHALLPDLQRGGR
                     AYILNTSSMMAYHAVASMALYSASKAFVLRWSRSLRLELKGTGISVTTLCPGSVITGF
                     TEQAGMQAMDDLARRFGSPPEPVAKAAVSAMLAGKAEVVPGVLNKLTHALQHLVPMRV
                     IESAASRIYINRLSRRKAGG"
     gene            complement(510..1142)
                     /locus_tag="PL106_12290"
     CDS             complement(510..1142)
                     /locus_tag="PL106_12290"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNA33895.1"
                     /translation="MRRAWRDAWHDPAFRRTFWSVLLVALPLVIAQRPFFGLVGARAG
                     THPWDPLIAGSVPMDVSPWVFVVLYASILFTVARALPLPRLLLRGICAYVLLLAMRMV
                     AMWLVALEAPVDAIPLVDPVTGLFYPDDTPFLKDLFFSGHTATLALMVPLSRGRIARL
                     TMMVCTALVAALVLLQHVHWTIDVFAAPVFTGLAWWASGLSLRLFGVKDD"
     gene            complement(1142..>1569)
                     /locus_tag="PL106_12295"
     CDS             complement(1142..>1569)
                     /locus_tag="PL106_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33896.1"
                     /translation="PRIALPAGNYFVSIRHRNHLGTMTATAVPVANGMQPIDLSDPAT
                     AVWGADARRIQGNKALLWCGDADQDHRVRYTGNGNDRDTILLTVGSTTPNNSVPGYLP
                     QDTNLNGHVRYTGNSNDRDAILLNVGAGTPSNQRVEQIP"
BASE COUNT          312 a          488 c          505 g          264 t
ORIGIN      
        1 caccggcaac ttgcccgtgg ttcattccga gattgaggcc ttggcagccc atctgcctgt
       61 gatgcgcctg aacatggagg tgccggtggc actcacccac gcgctcctgc ccgatctgca
      121 acgaggcggg cgggcgtaca tcctgaacac cagcagcatg atggcgtacc acgcagtggc
      181 gagcatggcc ctctactccg cgagcaaggc attcgtcctt cgctggagcc gctcgctgcg
      241 cctggagttg aaaggtaccg gaatatcggt taccaccttg tgcccgggct ccgtgatcac
      301 ggggttcacc gaacaggccg gcatgcaggc catggacgac ctggcacggc gcttcggttc
      361 acctccggaa ccggtggcca aggccgctgt atccgccatg ctcgccggaa aagccgaggt
      421 ggtgcccggt gtactgaaca agttgaccca tgcgctccag catctggtgc ccatgcgtgt
      481 gatcgagtcc gcagcgagcc gtatctacat caatcgtctt tcacgccgaa aagccggagg
      541 ctgagcccac tggcccacca agccaggccc gtaaagacgg gtgccgcgaa cacatcgatc
      601 gtccagtgaa catgctgcag caacaccagt gcggccacca gggcggtgca taccatcatc
      661 gtgagccggg cgatccgccc gcgtgagagc ggcaccatga gggcgagtgt ggccgtatga
      721 ccactgaaaa aaagatcctt caggaacggg gtgtcatccg gatagaacaa gcctgtcacc
      781 gggtcgacga gcggaatggc atccaccggt gcttcgagcg cgaccagcca catggccacc
      841 atgcgcattg ccaacagaag gacataggcg catatgccac gcaacaacag ccgcggcagg
      901 ggcagggcgc gagccactgt gaacaggatg gacgcgtaga gcaccacgaa cacccaaggc
      961 gagacatcca tcggtacgga accggcgatc aacggatccc aagggtgtgt ccctgccctt
     1021 gcaccgacca gaccgaagaa tgggcgttgt gcgatcacca gaggaagcgc gaccaggagc
     1081 acggaccaga aggtcctcct gaaggccggg tcgtgccatg catcgcgcca ggctcgccgc
     1141 atcaggggat ctgctccacg cgctggttac ttggcgtgcc ggccccgacg ttcagaagga
     1201 tggcgtcccg gtcgttggag ttgcccgtgt agcgcacatg cccattgagg ttggtatcct
     1261 gtggcaggta gccgggaacc gaattgttgg gcgtggtgct gcccacggtc agcaggatgg
     1321 tatcgcggtc gttgccgtta ccggtataac gcacgcggtg gtcctgatcg gcatccccgc
     1381 accaaagcag ggccttgttg ccttggatcc tccgggcatc agccccccat acggccgttg
     1441 cgggatcgct gaggtctatg ggctgcatgc cgttggcaac gggtacggcg gtggccgtca
     1501 tggtccccag atgattgcgg tgccggatgc tcacgaagta gttgcccgcg ggcagggcga
     1561 tacgcggcg
//
LOCUS       DAOQOC010000840         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62879_length_1567_cov_3.642857, whole genome shotgun sequence.
ACCESSION   DAOQOC010000840 DAOQOC010000000
VERSION     DAOQOC010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62879_length_1567_cov_3.642857"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1029)
                     /gene="murB"
                     /locus_tag="PL106_12300"
     CDS             complement(13..1029)
                     /gene="murB"
                     /locus_tag="PL106_12300"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNA33897.1"
                     /translation="MPIERNADLKPYHTFGVSARAAALAGFASEAELRALLNEPTLLG
                     LDRLILGGGSNLLFTRDWPGVVLRNDVPGIEVLSEDDERVTVRAGAGTVWHDLVLHTV
                     AQGWGGLENMSLIPGRVGAGPMQNIGAYGTELKDHFDHLEALRISDGEVIRFSKTECR
                     FGYRESFFKREGRDRFVIMRVAFTLNKRPQVNVGYGSIKSELEKRGIASPTIRDVSDA
                     VIAIRSSKLPDFRVLGNAGSFFKNPTVPATEADRIKRILPDLVTFPGEHGTVKLAAGQ
                     LIEKAGWKGFRENGYGVHKDQALVLVNHGGATGGQIFDLSTRVLESVRERFGVELERE
                     VNIF"
     gene            1074..>1567
                     /locus_tag="PL106_12305"
     CDS             1074..>1567
                     /locus_tag="PL106_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA33898.1"
                     /translation="MAPPHQRAIVSVTNDLVTDNRVHRTSMALVGLGYDVLLVGRILP
                     GSAPLERPYRTKRMRLLFNTGPLFYAEYNLRLFILLLFARADLLLANDLDTLLANHLA
                     RRGRKLVYDTHEFYTEVPELVGRPMVRRVWLAIERWIFPKLRTVITVNDSIAKAYRDR
                     YGNTV"
BASE COUNT          276 a          522 c          479 g          290 t
ORIGIN      
        1 gcgatccgag gctcaaaaaa tgttcacctc gcgttccaat tccacaccga accgttcgcg
       61 cacgctctcc agaacgcgtg tgctgaggtc gaagatctgg ccgcccgtgg ctccaccatg
      121 gttcaccagc accagcgcct ggtccttgtg tacgccgtaa ccattctcgc ggaaaccctt
      181 ccagcccgcc ttctcgatca gttggccggc cgccagcttc acggtgccgt gctccccggg
      241 gaaggtcacc agatcgggaa ggatcctttt gatccgatcg gcctcggttg cgggcacggt
      301 ggggttcttg aagaagctgc ccgcgttgcc cagcacgcgg aagtcgggca gtttgctgct
      361 gcggatggcg atcaccgcat cgctcacatc gcggatggtg ggggaggcga tgccccgttt
      421 ttccaactcg ctcttgatgc tgccgtagcc cacgttcacc tgtgggcgct tgttcagggt
      481 gaaggccacg cgcatgatca cgaagcggtc gcggccctcg cgtttgaaga agctctcgcg
      541 atagccgaac cggcactcgg tcttgctgaa gcggatcacc tcaccatcgc tgatgcgcaa
      601 cgcttcgaga tggtcgaaat gatccttgag ttccgtgccg tacgccccga tgttctgcat
      661 gggccccgcg cccacccggc cggggatcaa cgacatgttc tccagaccac cccagccttg
      721 ggccaccgtg tgcaacacca ggtcgtgcca cacggtaccg gctcctgcgc gcacggtcac
      781 gcgctcatcg tcctcggaga gcacttctat acccggcacg tcattgcgca gcaccacgcc
      841 tggccaatcg cgcgtgaaga ggaggttgct gccaccaccc aggatcaggc gatcaaggcc
      901 gagcagcgtc ggctcgttca gcaaggcgcg gagttcggcc tccgaggcga atccggccag
      961 ggcagctgca cgggcggata ccccgaaggt gtggtaaggt ttgaggtcgg cgttgcgttc
     1021 gatcggcata tcggggcgca agtttactgg tgggcgggcc gttacttcgc cgcatggccc
     1081 cgccgcatca acgcgccatt gtcagtgtca ccaacgatct ggtgacggac aaccgcgtac
     1141 accgcacgag catggccctg gtcggactgg gatacgatgt gctgctcgtg ggccggatcc
     1201 ttcccggcag cgcaccgctg gaaaggccct accgcacgaa gcggatgcgc ttgctcttca
     1261 acacagggcc gctcttctac gccgagtaca acctgcggct cttcattcta ctgctgttcg
     1321 cccgcgcgga cctgctgctg gcgaacgacc tggacacgct gctggccaac cacctggccc
     1381 gccgtggacg caagctcgtg tacgacaccc acgaattcta caccgaggtg cccgaactgg
     1441 tggggcggcc catggtacgc cgggtctggc tggccatcga gcggtggatc ttcccgaagc
     1501 tgaggaccgt gatcacggtg aacgacagca tcgccaaggc ctaccgcgac cggtacggga
     1561 acaccgt
//
LOCUS       DAOQOC010000841         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_62962_length_1566_cov_3.694904, whole genome shotgun sequence.
ACCESSION   DAOQOC010000841 DAOQOC010000000
VERSION     DAOQOC010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62962_length_1566_cov_3.694904"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PL106_12310"
     CDS             <1..492
                     /locus_tag="PL106_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNA33899.1"
                     /translation="HSAIGLDNVLHYYAHENTDAELRLLQAMLDHTDWPGNLPLPYIA
                     FGDQPLVERLGHGNHTGITMTSGGFYGPQGRQLRAVPSVDGLNDAFSSFRFPGPVDDQ
                     PLRITNYEMETSALYGLGGLLGHRTATICAVVANRLRKEYSKDHHAAIGRMIDQVLER
                     LVS"
     gene            635..>1566
                     /locus_tag="PL106_12315"
     CDS             635..>1566
                     /locus_tag="PL106_12315"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNA33900.1"
                     /translation="MNGPWITQVLPELVREGLITADQAERIRARYAADPQRSGNRMLL
                     VFAILGSLLVGLGIILIIAHNWDDLGRGTRTAIAFVPVVLGQGLVLYGIMRAPEVAAW
                     REGPAVLLASALCACVSLIAQIHHIGGSLEGYLLTCAVLILPLLYVPGSFCAALGYLA
                     MITWYAWIVRFEGFSTGERPWWFVPLLLAAVPFYLREARRNGTGAAFLWLSFFFALST
                     GLGSQLFYTDWTPAHVLGLAALAAAFTLVPWSHAGRELRTWPWVLIGGATMLLIMCVF
                     SFRPVWEEFDMKDRADWPLIGVYIAIGTVAYVLA"
BASE COUNT          252 a          508 c          476 g          330 t
ORIGIN      
        1 cacagcgcca tcgggcttga caacgtgctg cactattacg cccacgagaa caccgatgcc
       61 gagttgcgct tgttgcaggc catgctcgac cacaccgact ggcccggcaa cctgcccctg
      121 ccctacatcg cgttcggcga ccagccgctc gtggagcgcc tggggcacgg gaatcatacc
      181 gggatcacca tgacctccgg cggcttctat ggcccgcagg gacgccagct gcgcgcggtt
      241 cccagtgtgg acgggctcaa cgatgcattc agttccttcc gcttccccgg ccccgtcgac
      301 gatcaaccgt tgcggatcac caactacgag atggagacca gcgccttgta cggtctcggt
      361 gggcttttgg ggcatcgcac cgccaccatt tgcgccgtgg tggccaaccg cctgcggaag
      421 gagtactcca aggatcacca tgcggccatc ggccgcatga tcgatcaggt cctggaacgg
      481 ctggtcagct gagcctgcgg aacgcgagaa tgccagggcg accatcatcc tccttccggt
      541 tcggctcctt ttcacccgat cgtgcagcaa cggaaacgga cgtcaggatc cttgtcatca
      601 tcccatctga acctgatccg gtatcttcag cccgatgaac ggcccgtgga tcacccaggt
      661 gttgccggag cttgtgaggg agggcctgat caccgccgat caggccgaac gcatccgcgc
      721 gcgttatgca gcggatcctc aacggagcgg caaccgcatg ttgctcgtct tcgccattct
      781 tggcagcctg ctcgtgggct tggggatcat cctgatcatc gcgcacaatt gggatgatct
      841 tggtcggggc acgcgtacgg ccatcgcttt cgtcccggta gtgctggggc aaggactcgt
      901 cctgtacggg atcatgcgcg caccggaggt ggccgcatgg cgcgaaggcc ccgcggtgct
      961 gctcgccagt gccctttgcg catgtgtttc gctcatcgca cagatccatc acatcggagg
     1021 atcgctggag ggctacctcc tcacatgtgc cgtgctgatc cttccactgc tctacgttcc
     1081 gggatcattt tgtgcagcac tcggctacct ggccatgatc acctggtacg cgtggatcgt
     1141 acgcttcgag ggcttcagca cgggtgaacg accgtggtgg ttcgtcccct tgctgctggc
     1201 ggccgtaccg ttctacctgc gcgaagcacg gcgcaacggc accggtgccg cgttcctgtg
     1261 gctcagcttc ttcttcgcgt tgagtaccgg cctcggcagc cagctgttct acacggattg
     1321 gacaccggca cacgtcctcg gactcgctgc gcttgcggct gccttcaccc tggtgccgtg
     1381 gtcgcatgcg ggcagggagt tgcgtacctg gccttgggta ctgatcggtg gtgctaccat
     1441 gctgctcatc atgtgcgtct tcagcttccg acccgtatgg gaggagtttg atatgaagga
     1501 ccgcgccgac tggccgctga tcggggtgta catcgccatc gggaccgtcg catacgtgct
     1561 cgcgtt
//
LOCUS       DAOQOC010000842         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_63189_length_1563_cov_4.597480, whole genome shotgun sequence.
ACCESSION   DAOQOC010000842 DAOQOC010000000
VERSION     DAOQOC010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63189_length_1563_cov_4.597480"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PL106_12320"
     CDS             <1..544
                     /locus_tag="PL106_12320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNA33901.1"
                     /translation="VAKWRWYTNLFIVFLVSGLWHGANWTYVIWGAMHGAYLVLALLF
                     APFWEQVVGLTGLTRTPRLKRGLNVLATLILVTAAWVFFRASEVGDALPVYERLFSTP
                     WSLADLLMLRDGVNKGYFEATLFVALCFVLIDPLYDAWIKGELAAPSGWKGHLSFAAL
                     AVAILIFGYFGSTSFIYFQF"
     gene            544..1491
                     /locus_tag="PL106_12325"
     CDS             544..1491
                     /locus_tag="PL106_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33902.1"
                     /translation="MKRFVAQGLKFLLVVVVAYVVVFFALTWLRILGKPVIYRTNDYY
                     HLKGGNAWEQHNDWAKGARYDLVVIGSSHAYRGYDPRVFRERGYRMFNLGSSAQTPLS
                     TYAILEGLVPRERAPLVVIDVYEDAFVQAGIESVSELTRDMVDDRTAISLAADFRDLR
                     GLNMITLRMLDKGSPPSFADPTYVGDGAAIRRDSARSGVKYPVRGPLRIDPRQLERFI
                     DCLDLCDQRGIQVVLSSHPYPPQADRAQHTAFVAMVDSVIATRPEDRRPRRFDLVDAP
                     GFDGHDHFYDHTHLNQAGARAFTGMLIDSLEVKGILQRR"
BASE COUNT          272 a          483 c          495 g          313 t
ORIGIN      
        1 cgtggccaaa tggcgctggt acaccaacct gttcatcgta ttcctggtga gcggcttgtg
       61 gcacggggcg aactggacct acgtgatctg gggggccatg cacggtgcct acctggtgct
      121 ggccctgcta ttcgcgccat tttgggaaca agtcgttggc ttgaccgggc tcacgcgaac
      181 accgcggctg aaacgcgggc tgaatgtcct ggccaccctg atcctcgtga cggccgcctg
      241 ggtctttttc cgggccagcg aagtgggcga tgccctaccg gtgtatgaac gcttgttcag
      301 cacaccctgg tcgctggccg acctgctgat gttgcgtgat ggcgtgaaca aggggtactt
      361 cgaggccacc ttgttcgttg ccctgtgctt tgtgctcatc gatccgctgt acgatgcctg
      421 gatcaagggc gagctggccg ctccgagcgg ctggaaaggc catctcagtt tcgccgcact
      481 ggcggtggcc atccttatat tcgggtattt cggctccacc agtttcatct acttccagtt
      541 ctgatgaagc gcttcgttgc acagggcctg aagttcctcc ttgtcgtggt ggttgcctac
      601 gtcgtggtct tcttcgcgct cacgtggttg cgcatcctcg ggaaacccgt catctaccgc
      661 acgaacgatt actaccacct gaagggtggc aacgcctggg agcagcacaa cgactgggcg
      721 aaaggagcac gatacgacct ggtggtgatc ggatcctcgc acgcctaccg gggctacgat
      781 ccgcgtgtgt tccgcgagcg gggataccgc atgttcaacc tgggcagcag cgcacagaca
      841 ccattgagca catacgccat cctggaaggc ctggtacccc gggagcgcgc accgctggtg
      901 gtcatcgatg tgtacgagga tgccttcgtc caggccggga tcgaaagtgt gagcgaactg
      961 acgcgggaca tggtcgacga ccgcacggcg atcagcctcg ctgcggactt ccgcgacctg
     1021 cgtgggctga acatgatcac cttgcgcatg ctggacaagg gatcaccgcc ctcgttcgcc
     1081 gatccgacgt acgtggggga tggtgcggcc atccggcggg actccgcacg gtcaggggtg
     1141 aaatatccgg tccgtggccc cttgcggatc gatccccgtc aactcgagcg cttcatcgat
     1201 tgcctggacc tgtgcgatca acggggcatc caggtggtgc tcagctccca cccttatcca
     1261 ccgcaagccg atcgggcgca acacacggcc ttcgtggcca tggtcgacag tgtgatcgcc
     1321 acgcgaccag aggatcggcg gccgcgtcgc ttcgacctcg ttgacgcgcc cgggttcgat
     1381 ggccacgacc acttttacga ccacacgcac ctgaaccagg ccggggcgcg cgccttcacc
     1441 ggcatgttga tcgactcgct ggaggtgaag gggatattgc agcgccggtg agcgtgtgga
     1501 tctgaacggc aatgggaccg gtccacccgg taccggcaag cggccaagac ccggacgaac
     1561 cgg
//
LOCUS       DAOQOC010000843         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_63208_length_1563_cov_3.519894, whole genome shotgun sequence.
ACCESSION   DAOQOC010000843 DAOQOC010000000
VERSION     DAOQOC010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63208_length_1563_cov_3.519894"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PL106_12330"
     CDS             complement(<1..1170)
                     /locus_tag="PL106_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA33903.1"
                     /translation="MRPRMLLVLMVLVARAACGQYPLTRTIAVRDGQQGVQVSCMAQD
                     SLGLLWLGGPRGLFRTDGDRTDAALRTDNDAVTAVCGTDSGVLAALASGTMIRCEGLH
                     CDTLWTDTTLRSTPVRTLALAPDHGIWVGTHGGGAMLRLRGETYRLGAANGLSSDHVN
                     ALCLAGSDRMAIATDHGVVLMDLEGRRIAELGEDQGAPDNLVLDVCRDPDGRVWAGTD
                     RGGVFSFSSDGAVSVMEKIPAPGTVHQLAATIGRVWMGVEGQGLLVRDRTDGQTWYTT
                     SDPEEGSRVRMTDLLRDRDGAVWWCDGGAVLRRADPYVLVHTSHEGHDLRHVSAVATI
                     QGDRMAFAVRNTVLLRSAKIDEGQCMRTVVVPIDTATTITSLHADARRNLWAGTFG"
     gene            complement(1167..>1563)
                     /locus_tag="PL106_12335"
     CDS             complement(1167..>1563)
                     /locus_tag="PL106_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33904.1"
                     /translation="IGAQNFAVRIPMRAFKGFNSPLQQEHFYENYVRSGDFPNALFEG
                     RIIEEVDLARPGTYRVRAKGRLTFHGVIQERIIPCALAVDAHGIRVTGSFDIALADHN
                     IRIPRVVHQKLAPVTRVEVDLRFEAPAQR"
BASE COUNT          289 a          530 c          475 g          269 t
ORIGIN      
        1 cccgaatgtc cccgcccaca ggttccggcg cgcgtcggca tgcaaggagg tgatggtggt
       61 ggcggtatcg atcggcacga caacggtccg catgcactgc ccctcatcga ttttggcgga
      121 tcgcaggagc acggtgttcc gcaccgcgaa agccatgcga tcgccctgga tcgtggccac
      181 tgccgaaaca tgccggagat cgtgtccttc atgggaggtg tgtaccagca catacgggtc
      241 tgcacggcgg agcacggcac ccccatcaca ccaccacacc gcaccgtccc gatcacgaag
      301 caggtccgtc atgcgtacgc gcgacccctc ctccggatcg gaggtcgtgt accaggtttg
      361 tccatcggtc cggtcacgaa ccagcaggcc ctgcccttcc acccccatcc acacacggcc
      421 gatggtagcg gcgagctgat ggaccgtacc gggtgccggg atcttctcca ttacactgac
      481 cgcaccatcg gatgagaagc tgaacactcc cccgcgatcg gtaccggccc atacgcgtcc
      541 gtcaggatca cggcacacat cgagcaccag gttgtccggc gcgccctgat cctcgcccaa
      601 ttccgcgatc cggcggcctt cgaggtccat gaggacgacc ccatgatccg tggcgatcgc
      661 catacggtcg ctaccggcca ggcagagcgc attcacatgg tcgctgctca atccgttggc
      721 cgcacccagc cgatacgttt ctccccgaag ccggagcatg gcgcccccgc cgtgcgtgcc
      781 gacccagatc ccgtggtccg gtgccaaggc cagggtgcgc accggggtgg accgcagggt
      841 cgtatccgtc cagagcgtat cgcagtgcag gccttcgcac cggatcatgg tgccgctggc
      901 cagtgccgcc agtacgccac tgtccgttcc acataccgct gttacggcat cattgtcagt
      961 gcgcaatgcg gcatcggtgc ggtcaccatc ggtacggaaa aggccgcgcg ggccaccaag
     1021 ccagagcagg ccgaggctgt cctgggccat acagctgacc tgtacgccct gctggccatc
     1081 ccttacggca atggtccgcg tgagcggata ctgaccacac gccgcccggg cgaccagcac
     1141 catcagcaca agaagcatcc ggggcctcat cgctgggccg gagcttcgaa gcgcaggtcc
     1201 acttccacac gcgttaccgg tgccagcttt tgatggacca cgcgcgggat gcggatgttg
     1261 tgatcggcca gcgctatatc gaacgaaccg gtgacgcgga tgccatgggc gtccactgcg
     1321 agggcacagg ggatgatgcg ctcctggatc acaccgtgaa aggtgagacg gcctttggca
     1381 cgtacgcggt aggttcccgg tcgtgccaag tccacttctt caatgatgcg gccctcgaaa
     1441 agcgcattgg ggaagtcgcc gctcctcaca tagttctcgt agaaatgttc ctgctggagc
     1501 gggctgttga accccttgaa ggcccgcatc gggatgcgca cggcgaagtt ctgcgcaccg
     1561 atg
//
LOCUS       DAOQOC010000844         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_63277_length_1562_cov_5.425348, whole genome shotgun sequence.
ACCESSION   DAOQOC010000844 DAOQOC010000000
VERSION     DAOQOC010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63277_length_1562_cov_5.425348"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PL106_12340"
     CDS             complement(<1..767)
                     /locus_tag="PL106_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA33905.1"
                     /translation="MLIQYASDLHLEFSENRAWLKAHPMEPAAEVLLLAGDVLPLHQA
                     YGAPGLFKRLTQGFWEVIWLPGNHEYYGGRIEDKDLDTDIRLFRNIRIVNNRSVLYPG
                     VRILCTTLWTAIAQMNEGALRHNMNDYHAIRHGSGLLMPAHTTRFHAHCLAWLKSELT
                     KPFDGCTIVATHHVPTLQHYPPEYVGSALNQAFAVELEELIAGSGALAWIYGHHHRNI
                     PAFMIGGTRMFTNQVGYVAQNEQIGFDPAAVIGPGMHR"
     gene            complement(795..1160)
                     /locus_tag="PL106_12345"
     CDS             complement(795..1160)
                     /locus_tag="PL106_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33906.1"
                     /translation="MLKANFGDHVNPMGLFCSVTMPIKCPYCGTENVRECRTKELRGM
                     FLYEHWKVGDDVLADFPKELHCYTDCDCVESWESGTFPNGQEREGHRNWYVKVKLANG
                     VITGEYEVFKEVEDEDEYG"
     gene            1174..>1562
                     /locus_tag="PL106_12350"
     CDS             1174..>1562
                     /locus_tag="PL106_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33907.1"
                     /translation="MSQSRPFSKLKKQVEALFVPGLDLRVDCFVHAHRTQRSEVRVPR
                     YTLKLGEETIWHFPGDLPLKRETPHVWPYMVDISGLLRAYLDTPVDALLSHRFEQEQV
                     DLFHQGCREDGQHILSFGLELTPVLIAA"
BASE COUNT          322 a          470 c          427 g          343 t
ORIGIN      
        1 cgatgcattc ccggtccgat cacggccgcc ggatcgaagc cgatctgctc gttctgtgcc
       61 acatagccga cctggttggt gaacatgcgc gtgcccccga tcatgaatgc cgggatgttg
      121 cggtggtgat ggccgtagat ccacgccaac gcaccactac ccgcgatcag ctcctccagt
      181 tccaccgcaa aagcttgatt gagcgcactg cccacgtact ccgggggata gtgctgcaac
      241 gtgggaacat ggtgcgtggc cacgatggtg catccgtcga aaggtttagt gagttcgctc
      301 ttcagccacg caaggcaatg cgcatggaat cgcgtggtgt gcgcaggcat caacaatccg
      361 ctgccatggc ggatggcgtg gtagtcgttc atgttgtggc gcaaggcccc ttcgttcatc
      421 tgcgcaatgg ccgtccacaa cgtggtacac aagatgcgca caccgggata aagtaccgac
      481 cgattgttca cgatgcggat attccggaac agccggatat cggtatccag gtccttgtcc
      541 tcgatccgcc ctccgtagta ttcgtggttg ccgggcaacc agatcacctc ccagaagcct
      601 tgggtcaagc gcttgaacag tccgggagca ccatacgctt ggtgcaaggg cagcacatcg
      661 ccggccagga gcagcacttc tgcggccggt tccatgggat gtgccttcag ccatgcccgg
      721 ttctcggaga attccaaatg caggtcgctg gcgtactgga taagcatgtt cagcgaaacc
      781 gttcaaatag atcactagcc gtactcatct tcgtcctcca cctccttgaa cacctcgtac
      841 tccccggtga tcacgccatt ggccaacttc accttcacat accaattgcg gtggccttcc
      901 ctctcctggc cattgggaaa tgtcccgctt tcccaacttt ccacacagtc gcagtccgtg
      961 tagcaatgga gttccttggg gaagtccgcc aaaacatcat ccccgacctt ccaatgctca
     1021 tataggaaca tcccgcgcaa ctccttggtt cggcattccc gcacattttc ggtgccgcag
     1081 tatgggcatt ttatgggcat ggtaacggaa cagaagagac ccatggggtt gacgtgatcg
     1141 ccgaagttag ccttcagcat agcttcgcaa cacatgtcgc aatcgcgccc attctcgaag
     1201 ttgaagaagc aggtggaagc tttgttcgtt ccaggcctgg acctacgggt ggattgcttc
     1261 gtgcatgcgc atcgcacaca gcgcagcgag gtgcgggtgc cacgctacac gctgaaactt
     1321 ggcgaagaaa cgatctggca tttccccggc gacctcccgc taaaacggga aacgccacat
     1381 gtgtggccgt acatggtgga tatcagtggg ctgttgcgtg cgtacctgga cacccctgtc
     1441 gatgcccttc tctcccatcg tttcgagcag gaacaggtgg acctcttcca tcaagggtgc
     1501 cgggaggatg gacagcatat cctctccttt ggcttggaac tgacacccgt gctgatcgcg
     1561 gc
//
LOCUS       DAOQOC010000845         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_63495_length_1560_cov_3.095017, whole genome shotgun sequence.
ACCESSION   DAOQOC010000845 DAOQOC010000000
VERSION     DAOQOC010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63495_length_1560_cov_3.095017"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PL106_12355"
     CDS             complement(<1..474)
                     /locus_tag="PL106_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNA33908.1"
                     /translation="MATTRDPFRLGVFLLAGTLVLAVGLYLLGSKRNLFSRTIAVQAY
                     FQHVGGLRPGNNVRYMGINVGTVERIELTGDTAVRVVLAIRNDARAHIRANAVASVGT
                     DGLMGNRLVNLAPGDGTGGELKEGTVLPSSVPLDTELMLRTLDRTNENLATITEEV"
     gene            complement(477..1250)
                     /locus_tag="PL106_12360"
     CDS             complement(477..1250)
                     /locus_tag="PL106_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA33909.1"
                     /translation="MADPYPPGQPNAAAERVIRIQDLHLSFGSNHVIRGFSLDLHRGE
                     NVMVLGRSGSGKSVLIKCIVRLLRPDSGRIEVLGQDMLALDQDALDHLRVKIGFLFQS
                     SALYDSMTVQENLEFPLRRHWIATTPGEADALVGEVLAAVGLSETRDLYPSELSGGMK
                     RRIGLARTLILKPEIVLYDEPTTGLDPITGKEIVELILDLQRRYNTSSIIISHDLHVA
                     KLAANRIAVLHGGRNYLEGTYEELRRSEDELMKRFFVFM"
     gene            complement(1250..>1560)
                     /locus_tag="PL106_12365"
     CDS             complement(1250..>1560)
                     /locus_tag="PL106_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA33910.1"
                     /translation="GDALLVEMARRIGVNLRDTVSWELFYRQVFDSLEYSDLIPAFIK
                     SFFFGLAIGVVGCYKGFTTKKGTEGVGRSAHSAVVVASLLIFILDLLVVQITDLLGFT
                     "
BASE COUNT          287 a          490 c          482 g          301 t
ORIGIN      
        1 cacctcttca gtgatggtgg ccaggttctc gttggtccgg tccaaggtgc gcagcatcag
       61 ctccgtatcc aaagggacac tggagggcaa tacggttcct tccttcaatt cccctccggt
      121 gccatcgccg ggggcaaggt tcaccaagcg gttgcccatc aggccatcgg tgcccacgga
      181 agccaccgca ttggcccgga tgtgcgcacg ggcatcgttg cggatggcca gcaccacccg
      241 cacggcggtg tcccccgtca gttcaatgcg ctccacggtg cctacgttga tgcccatgta
      301 gcgcacgttg ttgcctggcc gcaagccgcc cacatgttgg aagtaggctt gtaccgcaat
      361 ggtgcggctg aagaggttgc gcttgctgcc cagcaggtaa agaccgaccg ccaacaccaa
      421 ggtgcccgcc agcaggaaca cgcccaagcg gaatggatct cttgttgtgg ccatggtcac
      481 atgaagacaa agaaacgttt catcagttca tcttccgatc ggcgcaattc ctcgtaggtg
      541 ccttccaggt agttgcggcc cccatgcagt accgcgatgc ggttcgcggc cagcttggcc
      601 acatgcaggt cgtgcgagat gatgatgctg ctggtgttgt agcggcgctg cagatcgagg
      661 atcagctcca cgatctcctt gccggtgatg gggtccagac cggtggtggg ctcgtcgtac
      721 agcacgatct ccggcttcag tatcaaggtc cttgccagcc cgatacgccg cttcattccg
      781 ccggacagct cgctggggta gaggtctcgg gtttcggaga ggcccaccgc cgcaagcact
      841 tcccccacca aggcgtcggc ctcgccgggt gtagtggcta tccagtggcg gcgcaagggg
      901 aactccaggt tttcctgcac cgtcatgctg tcgtacaggg cgctgctctg gaacaggaag
      961 ccgatcttca cccgcaggtg gtccagggca tcctggtcca atgccagcat gtcctgccca
     1021 agcacctcga tgcggccgct atcgggcctc agcaggcgca cgatgcactt gatgagcacg
     1081 cttttcccgc tgccgctgcg gcccagtacc atcacgttct cgccgcggtg caggtccagg
     1141 ctgaagccgc ggatcacatg gttgctgccg aaggacaggt gcaggtcctg gatgcggatc
     1201 acgcgctccg ctgccgcgtt gggctggccg ggtgggtatg gatctgccat caggtaaagc
     1261 ctaacaggtc cgtgatctgc accacgagca ggtccaggat gaagatgagc aatgaggcca
     1321 cgaccacggc actatgcgcg ctgcggccca cgccctcggt gcctttcttg gtggtgaagc
     1381 ccttgtagca gcccaccacg ccaatggcca ggccgaagaa gaagctcttg atgaaggcgg
     1441 ggatgagatc gctgtactcg agcgaatcga acacttggcg gtagaagagc tcccagctca
     1501 cggtatcgcg caggttcacg ccgatccgtc gtgccatctc gaccagcagg gcgtcgccca
//
LOCUS       DAOQOC010000846         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_63725_length_1557_cov_4.301598, whole genome shotgun sequence.
ACCESSION   DAOQOC010000846 DAOQOC010000000
VERSION     DAOQOC010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63725_length_1557_cov_4.301598"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PL106_12370"
     CDS             complement(<1..167)
                     /locus_tag="PL106_12370"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNA33911.1"
                     /translation="MWTENDNALRRDFTFRDFSEAFAFMTRVALLAEAMNHHPEWSNV
                     WNRVSITLRTHS"
     gene            complement(201..>1557)
                     /locus_tag="PL106_12375"
     CDS             complement(201..>1557)
                     /locus_tag="PL106_12375"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNA33912.1"
                     /translation="QVMNFSDPLRIRMRRASLDVIDAAMLASDKTRLQYANKQKGISN
                     AWKKWIGEVRGLKELDAVEEKRAFEAEFRKRDAQNGTARYTAALDSIDALYKVLPPYI
                     TARDLISELLGTGPEILRFANGFSTLVNDYDTLIAKDALQAELERLRKAAEGFYKEYD
                     PAVDRDIFEAQFRIYRSSMSPGLSAPEVVALGDEQRTPTGPYVDDLYERSIFDDQARM
                     TSLLKNFTKKARKRLAADPAFTLVQALLSTHATKVQPALADLNERLAVDMRRYMEGQI
                     ALFPERTFWPDANSTLRLSYGKVEGSIPRDGVEYTAFTTLDGAVAKYVPGDAEFDLPP
                     RLLELHRNKDYGRYGTDTGMPVCFTSSLHTTGGNSGSPVLNGRGELIGINFDRTWEST
                     MSDIRFDPDKCRNISVDIRYVLFTIDKLCGAQRLVDEMKLVRHVNAPMPMHLIPLPIH
                     R"
BASE COUNT          281 a          478 c          474 g          324 t
ORIGIN      
        1 gaatgcgtgc gcaaggtgat gctgacccgg ttccagacgt tgctccactc gggatggtgg
       61 ttcatggcct cggccaggag cgccacccgc gtcatgaagg cgaaggcctc gctgaaatcg
      121 cggaaggtga agtcgcgccg cagcgcgtta tcgttctcgg tccacatggt atacgcccat
      181 gccgggcgac ggcatccggt tcaacgatgt atgggaagtg ggatcaaatg catcggcatc
      241 ggcgcattga catgccgcac gagcttcatc tcatcaacga gccgttgcgc gccgcagagc
      301 ttgtcgatgg tgaagagcac ataacggatg tccacgctga tgttgcggca cttgtcggga
      361 tcgaagcgga tatcgctcat ggtgctttcc caggtacggt cgaaattgat cccgatgagt
      421 tctccgcgcc cattgagcac agggctgccg ctgttgccac ccgtggtgtg cagcgatgag
      481 gtgaagcaca cgggcatgcc ggtatcggtg ccgtaacgtc cgtagtcctt gttccggtgc
      541 agttccaaca ggcgtggtgg caggtcgaac tcggcatcac cgggcacata cttggccacg
      601 gcaccgtcca atgtggtgaa ggccgtgtat tccacaccat cgcgcgggat gctgccttcc
      661 accttgccgt agctgagccg caaggtgctg ttggcatcag gccagaaggt gcgctcagga
      721 aagagcgcga tctgcccttc catgtaccgg cgcatgtcca ccgccagccg ctcgttcagg
      781 tccgccagcg cgggttgcac cttggtggcg tgcgtggaca acagggcctg cacgagcgta
      841 aaggccggat ccgccgcgag ccgcttgcgt gctttcttcg tgaagttctt cagcaacgag
      901 gtcatcctgg cctgatcgtc gaagatggac cgctcgtaga ggtcgtccac gtacggtccg
      961 gtcggggtac gttgctcgtc gccgagcgcc accacttccg gtgccgacag ccccgggctc
     1021 atgctgctcc gatagatcct gaactgcgcc tcgaaaatgt cgcggtccac cgcagggtcg
     1081 tactccttgt aaaagccctc ggcagccttg cgcaatcgtt ccaactcggc ctgcagcgca
     1141 tccttggcga tcagcgtatc gtagtcgttc acgagggtgg aaaaaccatt ggcgaatcgc
     1201 aggatctccg gtcctgtgcc caacaactcc gatatcaggt cacgcgcggt gatgtatggc
     1261 ggaagcacct tgtacaacgc atcaatggaa tccaatgcgg ccgtgtaccg ggccgtcccg
     1321 ttctgcgcat cccgcttgcg gaactcggcc tcgaaggcac gcttctcctc caccgcatcc
     1381 agttccttga ggccccttac ctcaccgatc cacttcttcc acgcgttgct gatgcccttc
     1441 tgtttgttgg cgtactgcaa gcgggtcttg tcgctggcca gcatggccgc atcgatcaca
     1501 tcgagactgg cccggcgcat gcggatgcgg agtgggtcgg agaagttcat cacctgg
//
LOCUS       DAOQOC010000847         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64362_length_1550_cov_4.494983, whole genome shotgun sequence.
ACCESSION   DAOQOC010000847 DAOQOC010000000
VERSION     DAOQOC010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64362_length_1550_cov_4.494983"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1550
                     /locus_tag="PL106_12380"
     CDS             <1..>1550
                     /locus_tag="PL106_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33913.1"
                     /translation="PEPGTSGTLNVCSTAPLQDLFASLGGAPEMGGTWTSPGPIPHSG
                     QFNPASDPPGVYTYTIAGQAPCTDRSATVTVTVQAALSPGTSGDTTVCSNGTSINLKN
                     ILGTSATGTWAGPSAIGGNNLYSPVSMTPGTYTYTVAATGVCPAVNASVEVMETVAPV
                     AGGDGSYTVCSSGANVDLFTKLTGTPDVGGTWTAPGNVAFPTGVYVPGAPSNPPGTYT
                     YTVAGAFPCANDIATVTITQVPARNAGTNGTLTTCSNSAVSAMFGSLGGGPEVGGTWY
                     KPVPPGGVLVGGLYNPANATHPQGIYTYVLLGTAPCPNDTATVSVTEIQAPWAGNDNN
                     PFTLCNTSAPVNLITALGGSPNGGGSWLNPVNAPFPTGLFNPASGTPGLYKYIVPGTA
                     PCANDTGFVTVVVNQQPNAGISGDSTVCSNAANVPLLSVLGGTPGGGGTWSPSIGGGT
                     YDPGTGAPTTYTYTITALSPCVNVSAVATMVEVPAPDPGVSDAFQVCSNAVPFYLFPL
                     LDGSPDVGG"
BASE COUNT          296 a          512 c          481 g          261 t
ORIGIN      
        1 ccagagccgg gaacatccgg cacgctcaac gtgtgttcca cggctccact acaggatctg
       61 ttcgcttcgt tgggtggcgc tccggaaatg ggcggtacat ggacaagccc gggcccgatc
      121 ccgcacagcg gccaattcaa cccggcatcc gatcctccgg gcgtgtacac atataccatc
      181 gcgggccaag caccttgcac cgaccggtca gccacggtga ccgtcaccgt acaagccgcg
      241 ttgagccccg gaaccagcgg cgataccacg gtgtgttcca acggcacctc catcaatttg
      301 aagaacatcc tgggcacttc ggccacagga acctgggcgg gtcccagcgc gatcggcggg
      361 aacaacctgt acagcccggt ctcaatgaca cccggcacat atacgtacac cgtggccgcc
      421 accggggttt gtcccgctgt gaacgcctcg gtggaagtga tggagacggt ggccccggtc
      481 gcagggggtg acgggagcta cacggtttgc agtagcggtg cgaatgtgga cctcttcacg
      541 aaactcaccg gtacgcccga tgtgggcggt acctggaccg cgcccggcaa cgtggccttc
      601 ccgaccgggg tgtacgtgcc gggagcgcct tccaacccac cgggcacgta cacgtacacg
      661 gtcgcaggcg cattcccctg tgccaacgac atcgccacgg tgacgatcac ccaggtgcct
      721 gcaaggaatg ccggaaccaa cggtaccttg accacgtgca gcaacagcgc cgtgagcgcc
      781 atgttcggat cgctcggagg cggccccgag gtcggcggga cctggtacaa gccggtccct
      841 cccggtgggg tcctggtcgg cgggctgtac aaccccgcca acgccacgca tccacaaggc
      901 atatatacgt acgttcttct cggtacggcc ccatgcccca acgacacggc cacggtatcg
      961 gtaacggaga tccaggcgcc atgggccggc aatgacaaca acccgttcac cttatgcaac
     1021 accagcgcac cggtgaattt gatcaccgcg ctcggtgggt cgcccaatgg aggcggttcg
     1081 tggctcaacc ccgtgaacgc gccgttccct acgggcttgt tcaacccggc atcgggtaca
     1141 ccgggcctgt ataaatacat cgttcctggt acggcaccct gcgcaaacga cacgggcttc
     1201 gtcacggtgg tggtgaatca gcagcccaac gccggtatca gcggggacag caccgtgtgc
     1261 agcaatgcgg cgaacgtgcc gctcttgagc gtgctgggcg gaacgccggg cggtggtggt
     1321 acgtggagcc catcgatcgg tggcggcacc tacgacccgg gaacgggagc gcccaccacg
     1381 tacacgtaca cgatcacggc gttatcccct tgtgtgaacg tttctgccgt ggctaccatg
     1441 gtggaggtac ccgcaccgga ccccggcgtg agtgatgcat tccaggtgtg cagtaatgcg
     1501 gtcccgttct atctgttccc gctgctcgat ggaagccccg atgtgggcgg
//
LOCUS       DAOQOC010000848         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64366_length_1550_cov_4.236120, whole genome shotgun sequence.
ACCESSION   DAOQOC010000848 DAOQOC010000000
VERSION     DAOQOC010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64366_length_1550_cov_4.236120"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(579..>1550)
                     /locus_tag="PL106_12385"
     CDS             complement(579..>1550)
                     /locus_tag="PL106_12385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA33914.1"
                     /translation="PESIVIVGAGVIGCEFATIFSNFGRTRVHMIDRADRILPFEDAD
                     VSGTITRELERKGVIIHHGARLERMEAMPHGVEYTISGQDGRNTVTTELALLSVGRVP
                     NTDGLGLEHTGVERDTASGRIVVNDTRTSVPHIHVVGDATGSHMLVNLGELEGRHAVE
                     LLCGKAERPISYDNVSTIMFLDPEVAGVGLNEQECVRQGIAHRTARIDYACVARAIAM
                     RRTRGFFKLIVTDDDHMNVLGMRAVGEHASSAIQSVALMMRTGLGVRELADLTHPHPS
                     ITEGLQECARMLLGTSIFKSPVFGDKMYLGTWRPGTGTKDGAKVLAA"
BASE COUNT          276 a          470 c          491 g          313 t
ORIGIN      
        1 aagccattgt acctcctgca gtaaaatata ctcgggtctg atcgcgcgtt ccgcatgcgg
       61 aatgaagaat tctccgcact acttcaccgc gcaggtcgtt ccgtagcacc tgctccgcag
      121 gtcgtcctgc agaaggatcg gaacgtatca tcggaccacc ccttatcgtg tattggtata
      181 ataccccttc gcaatccagc atcgtccgat cgaaccatga aatcccatgc tggatccgac
      241 gaagcgcttc gacgggctca gcgtgacata ttggatggcc ccttactgcg aaatggtttg
      301 accccttcac tagacctccg tcccaattgc ccggaggaat gaataagacg tgtcacggtg
      361 agcctgccga accggtccct gcttcgccgt agcggctacg caaaggcgcg gtcgacggac
      421 tcaccgtgac acgctgatgg aagtacgacg gaggtttact tcctcctccc ggtcggatct
      481 gtggacaggg tgcgcttgag gaggagggtt gaagcgggag ccttggcctt ggcacacctt
      541 tgcttccgag catatcccat tcccggagca acaggctctc aggccgcaag gaccttggcg
      601 ccatccttgg tgccggtgcc gggcctccat gtacccaggt acattttgtc gccgaagacg
      661 gggctcttga agatggaggt gcccaacagc atgcgggcgc attcctgcaa cccctctgtg
      721 atgctggggt gcgggtgcgt gaggtcggcc agttcgcgca cgcccagtcc ggtgcgcatc
      781 atgagcgcca cggattggat ggcgctgctg gcgtgctcgc ccacggcgcg catgccgagc
      841 acgttcatgt gatcgtcgtc agtgacgatg agtttgaaga agccgcgcgt gcgacgcatg
      901 gcgatggcgc gggccacgca ggcgtagtcg atgcgcgcgg tacggtgggc gatgccctgg
      961 cgtacgcatt cctgttcgtt cagccccacg ccggccactt cggggtccag gaacatgatg
     1021 gtactgacat tgtcgtagct gatggggcgc tcggccttgc cgcagaggag ttccaccgcg
     1081 tgccggcctt cgagttcgcc gaggttcacc agcatgtgcg agcctgtcgc atcgcccacc
     1141 acgtggatgt gcggtacgct ggtgcgggta tcgttcacaa cgatccggcc gctggcggta
     1201 tcgcgctcca caccggtgtg ctccagtccc aggccatcgg tgttgggcac acgaccaacg
     1261 gaaaggaggg ccagctcggt ggtcacggtg ttacgtccgt cctggccgga aatggtgtac
     1321 tccacaccgt gcggcatggc ttccatccgc tcgagacggg cgccgtggtg gatgatgaca
     1381 cctttgcgtt cgagttcgcg ggtgatggtg ccactgacgt cggcatcctc aaagggcagg
     1441 atccggtcgg cacggtcgat catgtggaca cgggtgcgcc cgaagttgct gaagatggtg
     1501 gcgaactcgc acccgatcac gccggcgccc acgatcacga tgctctcggg
//
LOCUS       DAOQOC010000849         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64473_length_1549_cov_3.221553, whole genome shotgun sequence.
ACCESSION   DAOQOC010000849 DAOQOC010000000
VERSION     DAOQOC010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64473_length_1549_cov_3.221553"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1549
                     /locus_tag="PL106_12390"
     CDS             <1..>1549
                     /locus_tag="PL106_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA33915.1"
                     /translation="VAPRYGSLTRNGEGEVVGGSVMMLKGANSAQVVQAIEARIPQVQ
                     KALPEGLMIEPYLVRSKLVDRAIGTVEKNLIEGALIVVFVLVLFLGNWRAGLIVASVI
                     PLCMLFAIILMNAFGVSGNLMSLGAIDFGLIVDGAVIIVEATLHHLHAHFKGRTLSRR
                     EMNDAVYESASKIRTSAAFGEIIILIVYIPILTLVGIEGKMFRPMAITVSFAIIGALL
                     LSLTYVPMMSALFLPRNTAHTRNFSDRMMDRFQKWYDPIIAFVLRNRAKTVGVAVALF
                     AGAVLLFARMGGEFIPTLEEGDFAFHSFLPQGASLSQSVANNAKVEKILLGFPEVEQV
                     VGRTGSAEIPTDPMPLEATDLMIILKEKDQWTTARTREALQDTMLKALRVVPGVFFEA
                     TQPIQMRFNELMTGIRQDVAVKIYGENLDTLAAIAQQMAGIISEVEGAGEPQVEKVAG
                     LQQITVTFDRARVAQYGLNIRELNRTVRTAFAGEVSGAIYEDERRFDLVVRLAKQQRQ
                     SIEDVRTL"
BASE COUNT          290 a          500 c          502 g          257 t
ORIGIN      
        1 gtggcgccgc gctacggttc gctcacccgc aacggcgaag gcgaagtggt gggcggctcc
       61 gtgatgatgc tgaaaggcgc gaacagcgcg caggtggtgc aggcgatcga ggcgcgcatc
      121 ccacaggtac agaaggcgct gcccgagggc ctcatgatcg aaccctacct ggtgcgcagt
      181 aagctggtgg accgtgcgat cggcacggtg gagaagaacc tgatcgaggg cgccttgatc
      241 gtggtcttcg tgctggtgct cttcctgggc aactggcgcg cgggcctcat cgtggcatcg
      301 gtgatcccgc tgtgcatgct tttcgccatc atcctgatga acgccttcgg cgtgagcggc
      361 aacctgatga gcctcggtgc catcgacttc ggcctgatcg tggacggcgc ggtgatcatc
      421 gtggaagcca cgctgcacca cctgcacgcg cacttcaaag ggcgcaccct ctcgcgccgg
      481 gagatgaacg atgccgtgta cgagagcgcc agcaagatca ggaccagcgc ggccttcggc
      541 gagatcatca tcctcatcgt gtacatcccc atcctcacgc tggtgggcat cgaaggcaag
      601 atgttccggc ccatggccat caccgtgagc ttcgcgatca tcggcgcgct gctgctctcg
      661 ctcacatacg tgccgatgat gagcgcgctc ttcctgccgc gcaacaccgc acacacccgc
      721 aacttcagcg accgcatgat ggaccggttc cagaagtggt acgacccgat catcgccttc
      781 gtgttgcgca accgcgcgaa gaccgtgggt gtggcggtgg ccctcttcgc cggggcagtg
      841 ctcctcttcg cgcgcatggg cggcgagttc atccccaccc tggaagaagg cgacttcgcc
      901 ttccacagct tcctgccgca gggcgcctcc ttgagccaga gcgtggccaa caacgccaag
      961 gtggagaaga tcctgctggg cttccccgag gtggagcagg tggtgggccg caccggcagc
     1021 gccgagatcc ccaccgaccc catgccgctg gaggccaccg acctgatgat catcctcaag
     1081 gaaaaggatc aatggaccac ggcgcgtacg cgcgaggccc tgcaggacac catgctgaag
     1141 gccttgcgcg tggtgcccgg cgtgttcttc gaggccacac aacccatcca gatgcgcttc
     1201 aacgaactga tgaccggcat acgccaggac gtggcggtga agatctacgg cgagaacctc
     1261 gacacgctcg ccgcgatcgc acagcaaatg gcaggtatca tttcagaagt ggagggcgcg
     1321 ggcgaacccc aggtggagaa ggtcgcgggc ctgcagcaga tcaccgtcac cttcgaccgg
     1381 gcgcgcgtgg cgcagtacgg cctcaacatc cgcgagctga accgcacggt gcgcacggct
     1441 ttcgcgggcg aggtgagcgg cgccatttac gaggacgaac gccgcttcga cctggtggtg
     1501 cgcctggcga agcagcagcg ccagagcatc gaggatgtac gcacgctct
//
LOCUS       DAOQOC010000850         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64621_length_1548_cov_2.053583, whole genome shotgun sequence.
ACCESSION   DAOQOC010000850 DAOQOC010000000
VERSION     DAOQOC010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64621_length_1548_cov_2.053583"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..285
                     /locus_tag="PL106_12395"
     CDS             76..285
                     /locus_tag="PL106_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA33916.1"
                     /translation="MPAEPWVSVEDVARHLGVSHDTVYRWIEGKGLPAHKVGRLWKFK
                     LADVDEWVRSGGASDDAASRGDEPA"
     gene            complement(422..>1548)
                     /locus_tag="PL106_12400"
     CDS             complement(422..>1548)
                     /locus_tag="PL106_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33917.1"
                     /translation="TGTMQQLFDMYNAMANEIEADPALAPNIHLGCCGFTFNFLLNAF
                     ATQFMAAAEQADTRMDFIGYHPYSIGTPSGYDPGRTVIAAALRDLYRPNAELVNTEWG
                     LLGGVDREQWADLDYGLAKVRSMIGMLDEGVEIATQAVLTDIFDDTSSTCCPGVVQLW
                     PQVAIKNSTRPLIAMNMVRTATHRVGASSTDSAVVAGWTPGMDTLWVVATADTGIAPV
                     QVQLSIADLPWASARIERFELTETTIDVWNGPTLVEDDVQTDVWGDTLVHTVDQGSGR
                     LIIWRVTVAARVADASGFPSGASSTASTEPRRSGSRASAHSSARRGRPSGSCSHSSRK
                     MRNTGTAGSIAYRFTRLFSVCLRAAAVRLPSTFSSTTTRA"
BASE COUNT          260 a          529 c          496 g          263 t
ORIGIN      
        1 tgaggcgatc cacgtccgca tcaatccgac gtgataccct cccgctccgc acggcagtcc
       61 agccttggag tggtcatgcc cgcagaaccc tgggtcagcg tcgaggacgt ggcgcgccat
      121 ctcggcgtct cgcacgacac cgtgtaccgc tggatcgagg ggaaggggct gcccgctcac
      181 aaggtcggcc ggctgtggaa gttcaagctg gccgacgtgg acgagtgggt tcgcagcggc
      241 ggcgcgagcg acgacgcagc gtcgagggga gacgagcccg catgaaccag gcgaccacca
      301 gccaccgcgc gcggctcggc ttcctcgacg gggagcgcgg ggcgcacagc cacgcgacgc
      361 tgatggtgcg cgacctcgac gcgatcttcg gtgcgaccgc gcccgccgcc acgccggccg
      421 actacgcgcg ggtcgtcgtg gacgagaacg tgctgggcaa gcgcacggcc gccgctcgca
      481 agcacacgct gaacaacctc gtgaacctgt acgcgatgga cccggccgtg ccggtgttcc
      541 gcatcttccg ggagctgtgg gagcaggagc cggagggccg ccccctcctc gcgctgctgt
      601 gcgcgctcgc ccgtgacccg ctcctccggg gctcggtcga ggccgtgctg gacgcgccgc
      661 tcgggaagcc gctggcgtcc gccaccctcg ccgcgacggt gacccgccag atgatcagtc
      721 ttccgctgcc ctggtccacg gtgtgcacca gggtatcgcc ccaaacatcc gtctgtacat
      781 cgtcctccac cagggtcggc ccgttccaca catcgatggt cgtttcggtg agctcgaagc
      841 gttcgatccg tgcactggcc caaggcaggt ccgcgatgct gagttgcacc tgcaccggtg
      901 cgataccggt atccgcagtg gctaccaccc acagggtatc catgcccggc gtccaaccgg
      961 ccaccacggc gctatcggtg gatgaagcac cgacccgatg cgtcgcggtc cgcaccatgt
     1021 tcatggcgat caacggcctg gtgctgttct tgatggccac ctgcggccat aactgcacca
     1081 cgccggggca gcacgtgctg gaggtatcgt cgaaaatgtc cgtcagcacc gcttgggtcg
     1141 ctatttcaac gccttcatcc agcatgccga tcatggatcg caccttggcc aggccgtagt
     1201 ccagatcggc ccattgttcc cgatccaccc caccgagcag gccccattcg gtgttcacca
     1261 actccgcatt cgggcggtac aggtcccgca atgcagcggc gatgaccgtt cgcccgggat
     1321 catatccgga gggtgtcccg atggagtacg ggtggtatcc gatgaaatcc atgcgcgtat
     1381 ccgcctgttc ggccgctgcc atgaactgcg tagcgaaggc attgagcaag aagttgaagg
     1441 tgaatccgca acatccgaga tggatgttcg gcgcaagggc cggatccgcc tcgatctcgt
     1501 tcgccatggc attgtacatg tcgaagagct gctgcatggt gcccgtcc
//
LOCUS       DAOQOC010000851         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64651_length_1547_cov_4.367962, whole genome shotgun sequence.
ACCESSION   DAOQOC010000851 DAOQOC010000000
VERSION     DAOQOC010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64651_length_1547_cov_4.367962"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PL106_12405"
     CDS             complement(<1..736)
                     /locus_tag="PL106_12405"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNA33918.1"
                     /translation="MVSGTEQGGRRRKWRRRLIIGGVLVAMLLLVVAFLLPWLLKRYI
                     EEHSEEWIHRKVRIERIILNPFTFTYAVSGVTVHEPGSDQVFVSWDNIRVKVDLWKAF
                     RRNDWHFHHVRIVRPYVRIAQHKDRFNFSDLLELGGSEPKDSATSAPVRFSLEDIRLA
                     DGRVVYAADLLKEPAGITDLDAACTRITGESTRMDFDLSFALAQGGALKGGFMIDTEH
                     AVYAIDAGLTGFALPQLLPYLQDLMHT"
     gene            complement(822..1202)
                     /gene="ssb"
                     /locus_tag="PL106_12410"
     CDS             complement(822..1202)
                     /gene="ssb"
                     /locus_tag="PL106_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016361980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNA33919.1"
                     /translation="MNKISNTVRLIGNCGFDPEVRELAKGRKVARISVATHETYTNTE
                     GDRVTDTQWHTVVAWGQTAAALERLVRKGTGIALEGRLVHRSYQAKDGVKRFVTEIVV
                     SDFRLVGAKREAPQPEPAQLRIAA"
BASE COUNT          264 a          486 c          485 g          312 t
ORIGIN      
        1 tggtatgcat aagatcctgc aggtacggga gcagttgcgg cagggcgaac ccggtcaggc
       61 cggcatcgat ggcgtagacc gcatgttcgg tatcgatcat gaacccgccc ttcagcgcac
      121 ccccttgggc caaggcgaac gaaaggtcga aatccatgcg ggtgctttcg ccggtgatcc
      181 gggtgcaggc cgcgtcaagg tccgttatcc cggcgggttc cttcagcagg tcggcggcgt
      241 acaccactcg gccatcggca agccggatgt cctccaggct gaaccgtacc ggggcgctgg
      301 tcgcggaatc cttgggctcg ctaccgccca gttccaacag gtcgctgaag ttgaaccggt
      361 ccttgtgctg cgcgatgcgc acgtacggcc ttacgatgcg cacgtggtgg aagtgccagt
      421 cgttgcgtcg gaaggccttc cacagatcaa ccttcacccg gatgttgtcc cagctaacga
      481 agacctgatc gctgccgggc tcatgcaccg tgaccccgct taccgcgtac gtgaacgtga
      541 acgggttgag gatgatgcgc tcgatgcgca ccttgcgatg gatccactcc tcgctgtgct
      601 cttcaatgta gcgtttgagc agccagggca acaggaaggc gaccacgagc aacagcatgg
      661 ccaccagcac gcctccgatg atcaggcggc gacgccattt ccgccttctc cctccttgtt
      721 ccgtgccgct caccatgcgg acaaaggtga tgggggcgga tggaaaacaa agcggccccc
      781 accgcaaggc gaaggccgct ttcctgatgg gtaccgacca ctcaagccgc gatgcgcagt
      841 tgtgcaggct ccggctgtgg tgcttcccgc ttggcgccca ccaggcggaa gtcgctcacc
      901 acgatctcgg taacgaagcg cttcacgccg tccttggcct ggtacgagcg gtggaccaac
      961 cgaccttcca aggcgatacc cgtgcctttc cgcaccaagc gttccagtgc ggccgccgtc
     1021 tggccccaag ccaccacggt gtgccattgc gtgtccgtca cccggtcccc ctcggtgttg
     1081 gtgtaggtct cgtgggtggc caccgagatg cgggccactt tgcgcccttt ggccaattcg
     1141 cgtacttcag ggtcgaagcc gcagttgccg atgagccgca cggtgttgct gatcttgttc
     1201 atgttcgtag gtgttgggtt gtgttggctt gttcggatcc ggcattggtg tcgaattccg
     1261 tcggcaaacc ttcacctccc ccttgcccgg atccggtcgg cattgcactt ataggcggat
     1321 gcaggcgttt gttcaacggg tattccaggt ggcctccact tgccgatccc ggtatgggcg
     1381 ctactttcag gccgggtaac ggggtcaggc agcgcagaac ggggttcccg gcgtcttgcc
     1441 tcccggaaag gcccttgacc attgcgtgag ctgaccgaac gcgagctggt ggaaggctgc
     1501 cgaggcgggg accgccgtac acaggaactc ctgtacgggc ggtactc
//
LOCUS       DAOQOC010000852         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64657_length_1547_cov_3.808311, whole genome shotgun sequence.
ACCESSION   DAOQOC010000852 DAOQOC010000000
VERSION     DAOQOC010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64657_length_1547_cov_3.808311"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..938
                     /locus_tag="PL106_12415"
     CDS             150..938
                     /locus_tag="PL106_12415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNA33920.1"
                     /translation="MILILACMFAVVFALLVGMNRRLLVEARARSEQVQVAKRDLEAT
                     NRELRETMLSKEEKEVMIKEIHHRVKNNLQIVKSLIRFQQDQVSDERTRELFNECITR
                     VSAMALVHEQTYLTKDMANIPVDRYLDHLVRDLVHAYSVNVSLRMDINITVKTLSVDA
                     LVPLGLLINEVISNSFKYAFKGRDSGTITVHLHGDETDGLHMLIGDDGVGLKSRDGFH
                     RPNSLGMDLIHTLAGQLEGEMRLLDGPGTRYELVSTRQMRRKVA"
     gene            complement(1062..>1547)
                     /locus_tag="PL106_12420"
     CDS             complement(1062..>1547)
                     /locus_tag="PL106_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33921.1"
                     /translation="MKGVLVRLLKAGKQEAEKITRADGSYEFTLDRGWRYSVWYSKQD
                     RITKHIDVNTEGIPAYPDVPFYEMDVQMTMVEWINDFDFAIFDQALGEASFKQSVRNM
                     SWDLDYTEKMRPTLAKTMDEYEKTYRGYYKRKSARQPPTKRVAVVDSAGTVPVREEDR
                     K"
BASE COUNT          305 a          483 c          462 g          297 t
ORIGIN      
        1 cacgatctgc ccgcgcgcca cggcgagcga aagcccgcgc aaggcctgca cggccttgtt
       61 gtagacgacc tcgatgttgt tgatctcgag gatcggggcg ggcggcgcgc ccgtcatgcc
      121 ggcgcggccg ccgctgggac gaggcgcaga tgatcctgat cctggcgtgc atgttcgccg
      181 tggtcttcgc actgctggtg ggcatgaacc gtcgtctgct ggtggaggcc cgtgcgcgca
      241 gcgaacaagt gcaggttgcc aagcgcgacc tggaggccac caaccgcgaa ctgcgggaga
      301 cgatgctcag caaagaggag aaggaggtga tgatcaaaga gatccatcat cgggtgaaga
      361 acaacctcca gatcgtgaag agtttgatcc gatttcaaca ggatcaagta tcggacgagc
      421 gcacccgcga actcttcaac gaatgcatca cccgggtgag cgctatggcc ctggtgcacg
      481 agcagaccta cctgaccaag gacatggcca acattccggt cgaccgctac ctggaccatc
      541 tcgttcgcga tctggtgcat gcgtactcgg tgaacgtttc cctgcggatg gacatcaata
      601 tcaccgtgaa gaccctgagt gtggacgcat tggtgccact gggattgctc atcaatgagg
      661 tgatcagcaa cagcttcaag tacgcgttca aagggcgcga cagtgggacg atcaccgtcc
      721 acctgcacgg cgacgagacc gatggcctgc acatgctcat cggcgacgat ggcgtgggcc
      781 tgaagagccg cgacgggttc caccggccca acagcttggg catggacctg atccacacac
      841 tggccggcca actcgagggc gagatgcgcc tgctcgacgg tcccggcacc cggtacgaac
      901 tggtgagcac gcgccaaatg cggcgcaagg tggcgtgatc gtccgggacg accgctccaa
      961 aaaccagctt accgtatcaa gaaagtccga ggtcccgagc gcgcctcagc acggatcgcc
     1021 atgtgatcgg agcctcccct gccaaggctc gtcctggtcg ttcacttcct gtcctcttcc
     1081 cgcacgggaa cggtccccgc tgaatccacc acggccacgc gctttgtggg cggttgtcgg
     1141 gcggacttcc gcttgtaata gccgcggtag gtcttctcgt actcgtccat ggtcttggcc
     1201 agtgtggggc gcatcttctc ggtgtagtcc agatcccaac tcatgttccg cacgctctgt
     1261 ttgaacgatg cctcgcccag ggcctgatcg aagatcgcga aatcaaagtc gttgatccac
     1321 tccaccatgg tcatctgcac atccatctcg tagaacggca catcggggta cgccggtatg
     1381 ccctcggtgt tcacatcgat gtgtttcgtg atccggtcct gcttgctgta ccagacggag
     1441 taccgccagc cgcggtccag ggtgaactca taactgccat cagctcgagt gatcttctcg
     1501 gcctcctgct tgcccgcctt cagcaggcgc accagaacgc ccttcat
//
LOCUS       DAOQOC010000853         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64697_length_1547_cov_2.444370, whole genome shotgun sequence.
ACCESSION   DAOQOC010000853 DAOQOC010000000
VERSION     DAOQOC010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64697_length_1547_cov_2.444370"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1288)
                     /locus_tag="PL106_12425"
     CDS             complement(50..1288)
                     /locus_tag="PL106_12425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNA33922.1"
                     /translation="MLSGVDKTFSPADVARIFEMLFMSAGEGLVVVDHSGSMLMHNPR
                     LHTMFGYDAGDLIGRPIELLMPDAARDRHQHHRDNYNKHPVRRSMGIGLELWGKRKDG
                     TTFPVEVSLNHFEVDGVRYVMGLVTDVTLRRTAEDELHRTNQELERRVEERTLQLQAA
                     ERSVREALVKERELHALKSRFVAMASHEFRTPLSTIMSSTDLIARYVDGNERVDKHVT
                     RIRGKVREMTSMLNEFLSLERIEQGLVNCQPTHFDIVHLGIELIEELRTLTGDGQTIT
                     YDHEGSDRNVTLDRQMLGNVIVNLMTNAVKYSPQGKPITMRTVIKDGRLRVTVHDQGI
                     GVPEDDHQHLFERFFRGSNVTTIQGTGLGLNLVKRYLDIMQGGIGFSSVPGNTVFTVD
                     LPQHLPVVEGPGHVPAQTTI"
     gene            complement(1314..>1547)
                     /locus_tag="PL106_12430"
     CDS             complement(1314..>1547)
                     /locus_tag="PL106_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33923.1"
                     /translation="EERFAIGSNEKLQLVQETRLRPGMLGAIMRESSIHRFTNPGPGR
                     AVTLNLYAPPMSKWRVYDEGTGLSRVRPAGHIA"
BASE COUNT          268 a          476 c          458 g          345 t
ORIGIN      
        1 tctcccgcat gtccgcatca tcctctatca acagtatggt tttcatggtc tatatggtgg
       61 tttgggccgg aacatgcccc ggtccttcta cgaccggcag gtgctgaggc aggtccacgg
      121 tgaagacggt attcccgggc acactgctga acccgatccc gccctgcatg atgtccaggt
      181 agcgtttgac caggttcagt ccgagtccgg taccctggat ggtggtcaca ttgcttccac
      241 ggaagaagcg ttcgaagagg tgctggtgat cgtcctcggg gacaccgatg ccctggtcgt
      301 gaacggtgac ccgtagtcga ccgtccttga taacggtgcg catggtgatg ggcttaccct
      361 gtggtgagta tttcacggcg ttggtcatca ggttcacgat gacgttgccc agcatctggc
      421 ggtcaagggt cacattgcga tcgcttccct catgatcgta ggtgatggtc tgcccatcgc
      481 cggtaagggt acgcagctcc tcgatcaatt cgatgcccag atgcacgata tcaaagtggg
      541 tgggttgaca gttcaccagt ccctgttcga tccgctccaa tgaaaggaat tcgttgagca
      601 tggaggtcat ttcccgcacc ttgccgcgta tgcgggtcac gtgcttgtcc acgcgctcgt
      661 taccatcgac gtaccgcgcg atgaggtcgg tactgctcat gatggtgctg agcggtgtgc
      721 ggaattcatg gctggccatg gcaacgaacc ggcttttgag cgcatgcagt tcgcgttcct
      781 tcaccagggc ttcccgtacg ctgcgttcgg ccgcctgcag ctgcaaggtg cgctcctcca
      841 ccctgcgctc cagctcctgg ttggtccgat gcaactcatc ttcggccgtg cgccgtagcg
      901 tcacgtccgt taccagcccc atcacgtacc ggacgccgtc cacctcgaag tggttgaggc
      961 tcacctccac cgggaacgtg gtgccgtctt tccgcttacc ccacagctcc agcccgatac
     1021 ccatgctgcg gcgcaccgga tgcttgttgt aattgtcgcg atggtgctga tgccggtcgc
     1081 gtgcggcgtc gggcatcagc aactcgatcg gtcggccgat caggtcgccc gcgtcgtacc
     1141 cgaacatcgt atgcagccgg gggttgtgca tgagcatgga tccggaatgg tccactacca
     1201 cgagtccttc cccggcgctc atgaagagca tttcgaaaat gcgcgccacg tcggcgggcg
     1261 agaaggtctt atcgacaccg ctcagcatgt ggccaaagta ccactacgca cggttaagcg
     1321 atatggccgg ccggtcgcac ccgcgacaag cccgtccctt cgtcgtacac ccgccacttg
     1381 ctcatgggtg gagcgtacag attgagcgtt accgcccgcc cgggccccgg gttggtgaag
     1441 cggtgaatgg aactctcccg catgatcgcg ccaagcatgc ccggtcgcag ccgggtttcc
     1501 tgtaccagtt ggagcttttc gttggaaccg atcgcgaagc gctcctc
//
LOCUS       DAOQOC010000854         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64849_length_1545_cov_2.977852, whole genome shotgun sequence.
ACCESSION   DAOQOC010000854 DAOQOC010000000
VERSION     DAOQOC010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64849_length_1545_cov_2.977852"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /gene="atpF"
                     /locus_tag="PL106_12435"
     CDS             <1..502
                     /gene="atpF"
                     /locus_tag="PL106_12435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNA33924.1"
                     /translation="MNLVTPDIGLLFWMCLSFGIVVFLLSKFAWKPILGAVKTREQSI
                     ADALNEAKKMRDDMAKMSASNEEVMRQARAERDTLLKEARDIRDKEIAEAKAKAKAEA
                     DAMLGRAREDIRNEKNAAIVELRNQVADLSVQVAERILKEKLGDAAAQKALVDKVMTD
                     IDLRKS"
     gene            499..1059
                     /gene="atpH"
                     /locus_tag="PL106_12440"
     CDS             499..1059
                     /gene="atpH"
                     /locus_tag="PL106_12440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNA33925.1"
                     /translation="MNIAPVAYRYARSLMELALERGQLKAVQEDMRLVAGTCAASREL
                     QLLLKSPVIKADKKDRILDAVFAGKIGTITSSFMGILVRKGREAMLPAIAEAFEEIRR
                     EHDGVVTCHVTSAAPLDEEARTRAKDLAAKKYPGKSITLSEKVDQDLIGGVIVRIGDE
                     QYDGSVSRRLNDLRRKFSENPYIPEI"
     gene            1104..>1545
                     /locus_tag="PL106_12445"
     CDS             1104..>1545
                     /locus_tag="PL106_12445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988938.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNA33926.1"
                     /translation="MAQVQPAEVTAILKQELAGFRSEAELEEVGTVLQVGDGIARIYG
                     LKGVQSGELIEFNSGLRGIVLNLEEDNVGAVLLGSGIGVKEGDTVKRTQRIASIRVGE
                     QMVGRVVNTLGEPIDGKGPIGGELFEMPLERKAPGVIYRQPVKEP"
BASE COUNT          329 a          473 c          514 g          229 t
ORIGIN      
        1 catgaacctc gtcacccccg acatcggcct cctcttctgg atgtgcctgt ctttcggcat
       61 cgtggtgttc ttgctgagca agttcgcctg gaagcccatc ctcggcgccg tgaaaacccg
      121 tgaacagagc atcgccgatg cgctgaacga ggccaagaaa atgcgcgatg acatggccaa
      181 gatgtcggcc agcaacgagg aggtcatgcg ccaggcgcgt gccgaacgcg ataccctcct
      241 gaaggaagcg cgtgacatcc gcgacaagga gatcgcggag gccaaggcca aggccaaggc
      301 cgaggccgat gccatgctgg gtcgtgcccg tgaggacatc cgcaacgaga agaatgccgc
      361 catcgtggaa ctgcgcaatc aggtggccga cctgagtgtg caggtggccg agcgcatcct
      421 gaaggagaag ctcggtgacg cggccgcaca aaaggccctc gtggacaagg tgatgaccga
      481 catcgacctg cgcaagtcat gaatatcgcc ccggtcgcct accgctatgc ccgctcgctg
      541 atggagctcg ccctggagag gggtcagctg aaggccgtgc aggaggatat gcgcctggtg
      601 gccggtacct gtgcggccag ccgcgagctg cagctcctgc tgaaaagccc ggtgatcaag
      661 gccgacaaga aggacaggat cctcgatgcg gtcttcgccg ggaagatcgg cacgatcacc
      721 agcagcttca tgggcatcct ggtgcgcaag ggtcgcgagg ccatgctgcc agcgatcgcc
      781 gaagcctttg aggagatccg ccgcgaacac gacggagtgg tcacctgcca cgtgaccagt
      841 gcagccccgc tcgatgagga ggcccgcacc cgagcgaaag accttgcggc caaaaaatat
      901 cccggaaagt ccatcaccct ctcggagaag gtggaccagg acctgatcgg cggcgtgatc
      961 gtccgcatcg gtgacgagca gtacgacggc agcgtgagcc gccggttgaa cgacctgcgc
     1021 cgcaaattct ccgagaaccc gtacatcccc gagatctaac gcattccaga cccctcttcc
     1081 gccgaggcta cggcgggtga aacatggccc aagtgcaacc cgccgaagtg acggcgatcc
     1141 tgaaacaaga actcgccggt ttccgctcag aggccgaact cgaagaggtc ggtaccgtgc
     1201 tgcaagtagg cgacggcatc gcccgcatct acggcctgaa gggcgtgcag agcggtgagc
     1261 tgatcgagtt caacagcggc ctgcgcggca tcgtgctgaa cctggaggag gacaacgtgg
     1321 gcgccgtgct gctcggttcg ggcatcggcg tgaaggaggg tgatacggtg aagcgcacgc
     1381 agcgcatcgc cagcatccga gtgggcgagc agatggtggg ccgcgtggtg aacacgctgg
     1441 gcgaacccat cgacggcaag ggccccatcg gcggcgagct gttcgagatg ccgctggagc
     1501 gcaaggcccc gggcgtgatc taccgccagc cggtgaagga gccgc
//
LOCUS       DAOQOC010000855         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64855_length_1545_cov_2.873826, whole genome shotgun sequence.
ACCESSION   DAOQOC010000855 DAOQOC010000000
VERSION     DAOQOC010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64855_length_1545_cov_2.873826"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PL106_12450"
     CDS             complement(<1..1514)
                     /locus_tag="PL106_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNA33927.1"
                     /translation="MERLADHQLDLVEERRAGDHLGQLRSGSYADHSKLASGSWYRFS
                     VYDDGVYKLSWTFLRDLGINMDDLSSDRINVYGNHAGLLPYKGSHIPANDLLANAIEV
                     VDGGDGVFGPNDYILFYASGAQDWVWNATDARFEHTKNTYTDSACYFIGLDIEDPERI
                     MSTVLSTEPATDQVTTFADRQTIDRDLVNLLKSGRTWVGDTYNTLTPTYNYNFSVPNL
                     RSTEPWCVKIDVLSRTIQPGLSTWNMTVSGQPFSFQDSNITGHYAGPYARQTVKTFCG
                     TTAANNLQVNVAFDGADDISSVGWMNFLELNCRRDLRMVGDQLRFRDPVSMGAGHVGE
                     FTVDQASSVTRIWDITVPWSASSVPYTDNGAQKVFRVATDTLREFIAFKNSGFLTPVA
                     VGPVPNQDLHAVAEADMVIVSPELFLGQAQRLADRRTEEGLSVVLVTPQQIYNEFSSG
                     QRDATAIKRFMKMLYDRAGMDPELMTRYLLLFGDGSYNNISIAGSNQNWIPSYQS"
BASE COUNT          285 a          482 c          466 g          312 t
ORIGIN      
        1 gattgatagg acgggatcca gttctggttg ctgccggcga tactgatatt gttgtacgac
       61 ccgtcgccga acaacaggag gtagcgcgtc atcagttccg ggtccatgcc cgcgcgatcg
      121 tacagcatct tcataaagcg cttgatcgcc gtagcatcgc gctggccgct gctgaactcg
      181 ttgtagatct gctggggcgt caccagcacc accgacaggc cctcctcggt ccgacgatcg
      241 gccagccgtt gcgcctggcc aaggaacagt tcgggggata cgatcaccat gtcggcctcg
      301 gcaacggcgt gcaggtcctg gttggggacg ggtcccaccg ctaccggtgt cagaaagccg
      361 ctgttcttga acgcgatgaa ctcccgcaac gtgtcggtgg ccacgcgaaa gaccttctgc
      421 gcaccattgt ccgtgtacgg aacactgctg gccgaccacg gcaccgtgat gtcccagatg
      481 cgggtcacgc tcgaggcctg gtccaccgtg aattcgccca catggcccgc acccatgctc
      541 accggatcgc ggaatcgcaa ctggtcaccg accatgcgca ggtcgcgtcg gcagttcagc
      601 tccaaaaagt tcatccagcc gaccgaactg atgtcgtccg ccccatcgaa cgcgacgttc
      661 acctgcaggt tgttggccgc cgtggtgcca cagaaggtct tcaccgtctg ccgggcgtag
      721 ggacccgcat aatgacctgt aatgttactg tcctggaagg agaagggctg cccgctcacc
      781 gtcatgttcc acgtgctcag gccaggttgg atcgtccggc tcagcacgtc gatcttcacg
      841 caccagggtt cggtgctgcg caggttgggc acactgaagt tgtagttgta cgtcggggtc
      901 aacgtgttgt acgtatcgcc cacccaggtc cggccactct tcagcaggtt caccagatcg
      961 cggtcgatcg tctgccggtc agcaaaggtg gtcacttgat ccgtggccgg ctccgtgctc
     1021 aacacggtgc tcatgatccg ttcggggtcc tcgatgtcga gcccgatgaa gtagcacgcg
     1081 gagtccgtgt aggtgttctt ggtatgctcg aagcgcgcat ccgtcgcgtt ccacacccag
     1141 tcctgggcac cggaggcata gaaaaggatg tagtcgttcg gtccgaagac accgtccccg
     1201 ccatccacca cttcgatcgc attggccagc aggtcattgg ccgggatgtg gctgcccttg
     1261 taaggaagca ggcccgcgtg gttgccgtac acattgatgc ggtccgatga caggtcatcc
     1321 atattgatcc ccaggtcccg caggaaggtc caggagagtt tgtacacccc gtcgtcgtac
     1381 accgaaaagc ggtaccagga tccgctggcg agcttggaat gatcggcata actgccactg
     1441 cgcaactgcc ccaggtggtc gcccgcgcga cgctcctcca ccaggtccaa ttggtgatcg
     1501 gccagccgtt ccatagcccc ggtgatcggg ttgcgtcggt agggg
//
LOCUS       DAOQOC010000856         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_64902_length_1544_cov_5.042982, whole genome shotgun sequence.
ACCESSION   DAOQOC010000856 DAOQOC010000000
VERSION     DAOQOC010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64902_length_1544_cov_5.042982"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>1544)
                     /locus_tag="PL106_12455"
     CDS             complement(156..>1544)
                     /locus_tag="PL106_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA33928.1"
                     /translation="MFRADPRASTRSRNYYEPDIHVPSFTVKWRPSSRTQLRWTTSAI
                     LGARYSTLFDRPATIADAIDSTTLRYAARQVDIDYFHSFTSELRARHDWTLNGRTSTW
                     AAGAQLMRNDLNRRQMGKGTTGSEYDLTLTVPSWRRDLHYRTENIAFFAESRWSMTDR
                     LAVVTGARLELGSTELDGRTTYYPGDELPTTIAHRFPLFGASAEYRFNGTTNAYAGWS
                     QAYRPVVLKDIIPASVFESTDKDLKDVTGHNAEAGVRGTWKALRWDVSVFQLVQRDRL
                     GTLASEDSAGNVLVRRTNTGDSRARGVECFVQADIRIAPHTAVSLFTSTAWMDARYLD
                     AMIRSGNGNKDISGNKVECAPEWTNRSGITLRYHNVRISALFSHVSETFADALNTREP
                     NASGTVGLVPAYSLLDLNVTWMLNSRLEFRANLNNVLDERYFTKRPQFYPGPGIWPSD
                     GRGWSVSVALRV"
BASE COUNT          276 a          475 c          506 g          287 t
ORIGIN      
        1 actccacact ttcgggaccg gcgtactggg catggcccgt acatgcgacg agcaggggga
       61 gaaagaggat ctggcgcata gtgaatggtc gcgcaagcta tccaaggctt tggatcggca
      121 tgcacggaag cggagcccac gtgcaatacg cctcttcaaa cccgcaacgc cacactcacc
      181 gaccatcccc gaccgtcgct gggccagata ccggggccgg gatagaactg tggccgcttg
      241 gtgaagtacc gctcatccag gacgttgttc aggttggcgc ggaactccag gcggctgttc
      301 agcatccatg tgacgttcag gtccagcaga ctgtacgcgg gcaccaggcc cacggtaccg
      361 gacgcgttgg gctcacgcgt attcagcgcg tccgcgaagg tctctgatac atggctgaac
      421 agcgcactga tccgcacgtt gtggtaccgc aacgtgatgc cgctccggtt cgtccactcc
      481 ggggcgcatt ccaccttgtt gccgctgatg tccttgttgc cattgcctga ccttatcatc
      541 gcatccaggt agcgggcatc catccacgcg gtggaggtga agagcgagac agcggtatgc
      601 ggtgcgatcc ggatatcggc ctgcacgaag cactccacgc ctcgcgcgcg cgaatcgccg
      661 gtgttggtgc gccttacgag gacgttgccc gcgctgtcct cgctggccag agtgccaagg
      721 cggtcgcgct ggacgagctg gaacacgctc acgtcccagc ggagggcttt ccatgtgccg
      781 cgcacgcccg cctcggcatt gtggccggtg acatccttca ggtccttgtc cgtcgactcg
      841 aacaccgagg ccggtataat atccttgagc accacaggtc ggtacgcctg cgaccagccg
      901 gcataggcgt tcgttgtccc gttgaaccgg tattccgccg aggcgccgaa cagcgggaac
      961 cggtgcgcga tggtggtggg cagctcgtcc ccggggtaat aggtggtgcg gccgtcgagc
     1021 tccgtggagc ccagttccaa gcgcgcaccg gtgaccacgg cgagccgatc ggtcatgctc
     1081 caccggctct cggcgaaaaa ggcgatgttc tccgtgcggt agtgcaggtc gcgcctccag
     1141 ctcggcacgg tgagggtcag gtcgtattcc gaaccggtcg tgcccttgcc catctggcga
     1201 cggttgaggt cgttgcgcat caactgcgcg ccagccgccc aagtgctcgt tcgaccgttg
     1261 agcgtccagt catgccgcgc acgcaactcg ctggtgaagc tgtggaagta gtcgatatcc
     1321 acctggcgcg ccgcgtaccg gagcgttgta ctgtcgatgg catcggcaat ggtggcgggc
     1381 cggtcgaaga gcgtgctgta gcgtgcacca aggatggccg aggtggtcca acgcaactgc
     1441 gtgcgggatg atggccgcca cttcacggtg aaggacggca catggatgtc aggttcataa
     1501 taattgcgcg aacgggtact ggcacgcggg tcggcacgga acat
//
LOCUS       DAOQOC010000857         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65020_length_1543_cov_3.742608, whole genome shotgun sequence.
ACCESSION   DAOQOC010000857 DAOQOC010000000
VERSION     DAOQOC010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65020_length_1543_cov_3.742608"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PL106_12460"
     CDS             <1..632
                     /locus_tag="PL106_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA33929.1"
                     /translation="IAGLKLHLQTLQRHDLPDEQREALTRHVRTEVDRLHGLTEKILL
                     ATKLDDPHMALAPAETDVAALLRAVKVAAEAGYARGHSLRMNAPERFVTRTDADALRS
                     VTVNLLENACKYAPTGSMIDIELENIGPHWELRVSDEGPGIPEADRTTVFRKFKRVGN
                     EETRHAKGTGLGLYITHRLTRALGGHIGYRPRPGGGSIFAASFPLRGNK"
     gene            619..>1543
                     /gene="gpmI"
                     /locus_tag="PL106_12465"
     CDS             619..>1543
                     /gene="gpmI"
                     /locus_tag="PL106_12465"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNA33930.1"
                     /translation="METSKTDVLPHKAALIILDGWGIGAGDSTDAIAQARTPFISGLL
                     ATAPHAKLLTHGPNVGLPEGQMGNSEVGHLNIGAGRVVYQELVRIDRAVNDGELERNT
                     VLREALEIAQAPGKRLHLMGLVGHGGVHAMQRHLVALCGIVGRAGVKEAFLHAFTDGR
                     DTDPKSALGFIKELERHIADTPVRIASVCGRYWAMDRDKRWERVKRAYDLLVHGTGER
                     APDAVAAIERSYANSITDEFVEPCAITGADGAPRATIRPGDVVLCFNFRTDRCREITQ
                     ALTQRPFPDQGMIPLPLHYVTMTEYDSTYKDV"
BASE COUNT          315 a          489 c          493 g          246 t
ORIGIN      
        1 ccattgccgg cttgaaattg catctgcaga ccttgcagcg ccacgacctg cctgatgagc
       61 agcgtgaagc actcacaagg cacgtacgaa cggaggtgga tcggctgcac ggcctcaccg
      121 aaaagatcct gctggccacc aagctggacg atccgcacat ggccttggca ccagcggaga
      181 cggatgtggc cgcattgctt cgcgcggtga aggtcgccgc ggaggccgga tacgcgcgcg
      241 ggcattcgct ccggatgaac gcaccggaaa ggtttgtgac ccgtaccgac gccgatgcct
      301 tgcgttcagt gaccgtgaac ctgttggaga acgcgtgcaa atacgcaccg acgggcagca
      361 tgatcgatat cgagctggag aacatcggac cacattggga attgcgggtg agcgacgagg
      421 gccccggcat cccggaggcc gaccgcacga ccgtattccg caaattcaaa cgggtcggca
      481 acgaagaaac acgccatgcg aaaggcaccg gacttggcct ctacatcact catcggttga
      541 cacgtgcgct gggtggacat atcggatacc ggccacgccc cggtggcggg tctattttcg
      601 cggcctcatt cccgctccgt ggaaacaagt aagaccgacg tcctgccgca caaggccgca
      661 ttgatcatcc tggatggttg gggcatcggg gccggcgaca gcaccgatgc gatcgcccaa
      721 gcccggaccc cgttcatcag cggcctgctc gccacggccc cgcacgccaa actgctcacg
      781 cacggcccca acgtaggcct gcccgagggt cagatgggca acagcgaggt gggtcacctc
      841 aacatcggcg cgggccgtgt ggtgtatcag gagctggtcc gcatcgaccg tgcggtgaat
      901 gatggcgaac tggagcggaa caccgtgttg cgggaggccc tggagattgc gcaagcaccc
      961 gggaagcggc tgcacctcat ggggcttgtc ggccacggtg gtgtgcatgc catgcaacgg
     1021 cacttggtgg cactctgcgg catcgtcggt cgagcgggcg tgaaagaggc cttcctccat
     1081 gctttcaccg atgggcgcga caccgacccg aagagcgcat tgggcttcat caaagagttg
     1141 gaacgccaca tcgctgatac gcccgtgcgc atcgccagcg tgtgcggccg ctactgggcc
     1201 atggaccgcg acaagcgttg ggagcgcgtg aaacgcgcgt acgacctgct ggtacatgga
     1261 acgggagaac gggcgcccga tgccgtggcc gccattgagc gcagctacgc gaacagcatc
     1321 accgacgagt tcgtggagcc atgcgcgatc accggggccg atggcgcgcc acgggccacg
     1381 atccgtccgg gtgacgtggt cctctgcttc aacttccgca ccgaccgctg ccgcgagatc
     1441 acgcaggcgc tcacccaacg tccattccct gaccagggaa tgatcccgct accgctgcat
     1501 tacgtcacca tgacggaata cgacagcacg tacaaggacg tac
//
LOCUS       DAOQOC010000858         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65021_length_1543_cov_3.719758, whole genome shotgun sequence.
ACCESSION   DAOQOC010000858 DAOQOC010000000
VERSION     DAOQOC010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65021_length_1543_cov_3.719758"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..471)
                     /locus_tag="PL106_12470"
     CDS             complement(127..471)
                     /locus_tag="PL106_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33931.1"
                     /translation="MRALLLLAALALLFPGCYYDNEEDLYGPSCDASVFTYSGQIEPI
                     IAANCQLPACHGINGENGELVTYEQVKAVVDDGTFRQAVIVEKRMPDGGALTNCELTL
                     IENWLNAGAPNN"
     gene            complement(480..1196)
                     /locus_tag="PL106_12475"
     CDS             complement(480..1196)
                     /locus_tag="PL106_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33932.1"
                     /translation="MLPFLMVIGILFNSCKHEPLVPPQAPAGLNGGGGVEPEPVDTCD
                     PNIIWFEQEVLPIFLTHCTMSGCHGDVSPNDADMVFTSHAGLNSDPNMISDIWEAINE
                     DPDDNDHMPPLVDDLNIPLDQLTPEEIAIIGAWIDQGANNNSCDGGCDMTAITYSGTI
                     LPIVQDRCQGSCHAGNNPDGGLDFTTWTDLNAVAADGTLALAIQHQAGAEPMPQSGPA
                     LSQCRIDQFLAWIQDGAPNN"
     gene            complement(1327..>1543)
                     /locus_tag="PL106_12480"
     CDS             complement(1327..>1543)
                     /locus_tag="PL106_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33933.1"
                     /translation="DFAMSFRSLDHLTFLRPKDRRRLLSTDDEHLTGLVMELNRLPHY
                     NNRLDAIQLVAAILRGRRRTGTAGQGH"
BASE COUNT          295 a          425 c          501 g          322 t
ORIGIN      
        1 gctcacggtc agttcggcgt tctgtccctg ggccgatgcc accggtcgtt ggtacatgcg
       61 gtagcccacg aatgcaccaa tggccgcaat ggccacgacc gcgaggagga gataacgtct
      121 catggttcag ttgttcgggg cacccgcgtt cagccagttc tcgatcaggg tgagttcgca
      181 attggtcagg gctccgccat cgggcatgcg tttctccacg atcacggcct ggcggaatgt
      241 cccatcgtca accacggctt tcacctgctc gtacgtgacc agctcaccgt tctcgccgtt
      301 gatgccatgg caggccggta attgacagtt ggcagcgatg atgggctcga tctgcccgct
      361 gtacgtgaac acgctggcat cgcacgatgg gccatacagg tcctcctcgt tgtcatagta
      421 acaacccggg aagagcagtg cgagtgcggc aaggaggagt aaagcacgca tctggggtat
      481 caattgttcg gggctccgtc ctggatccat gcgaggaatt ggtctatccg gcactggctc
      541 agggccggcc cgctctgtgg catgggctcg gcccctgcct ggtgctggat ggccagggcg
      601 agggtaccat cagcggccac cgcgttcagg tcggtccagg tggtgaagtc caagccgcca
      661 tcggggttgt tgccggcatg gcaactgccc tggcagcggt cctgcacaat gggaaggatc
      721 gtgcccgaat aggtgatggc ggtcatgtcg cagccaccat cgcaggagtt gttgttcgca
      781 ccctgatcga tccaggcacc aatgatagcg atctcctccg gggtcagctg gtccaaaggg
      841 atgttcaggt catccaccaa gggcggcata tggtcgttat cgtccgggtc ctcgttgatg
      901 gcttcccaaa tatcagagat catattcgga tcgctgttga gaccggcatg gctcgtgaac
      961 accatgtcgg cgtcgttggg ggacacatcg ccatggcagc cgctcatggt gcagtgggtg
     1021 aggaagatcg gcagcacctc ctgctcgaac cagatgatgt tcggatcgca ggtgtccacc
     1081 ggttcgggtt ccactcctcc cccaccgttc agacctgccg gcgcttgcgg cgggacaagg
     1141 ggttcgtgct tgcaggagtt gaacaggatg ccgatcacca tgaggaacgg caacattcca
     1201 gtgaacaagc ggtatctcat tggaggggtg ggtcccgggt gatgcggagc ggtgcgaaaa
     1261 tgcatcgtgg aaggggtttg gaaagacgac ttccggacgg gccgggaaat gatggggatg
     1321 agtcggtcaa tgtccttgac cggccgtacc ggtccgtcgc cttcctcgaa gtatggcggc
     1381 taccagttgg atggcatcca gtcggttgtt gtaatgagga agtcggttca gttccatcac
     1441 cagaccggtc aggtgctcat catcggtgct caacaaacga cggcgatcct tggggcgcag
     1501 gaaggtgagg tgatccagtg aacggaagct catcgcgaag tcc
//
LOCUS       DAOQOC010000859         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65053_length_1543_cov_2.731183, whole genome shotgun sequence.
ACCESSION   DAOQOC010000859 DAOQOC010000000
VERSION     DAOQOC010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65053_length_1543_cov_2.731183"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PL106_12485"
     CDS             <1..178
                     /locus_tag="PL106_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNA33934.1"
                     /translation="PGGLAFEEATYLLSRLAASGRRIIGFDLVEVTPGRNDEWDANVG
                     ARLLWHLCGVMANP"
     gene            257..1069
                     /locus_tag="PL106_12490"
     CDS             257..1069
                     /locus_tag="PL106_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA33935.1"
                     /translation="MEILGYLCAALMGLALGMIGGGGSILTVPILVYLFGVDPVHAAA
                     YSLFIVGLTALVGGLGHLRMGQVDQRSVFAFGLPSIFGVAFTRRFIVPALPDPLLQVG
                     AWRIGLDPVLLLLFAVLMLLAARQMLRAAEPADGAPSDVPANGGGLALRGLLTGVATS
                     LVGAGGGFLIVPALMAFARLPMKKAVGTSLTIIALNGLIGAASDPHMAQHPDWTLILV
                     VAGIAIAGILVGSVLGHRVPNERLKPAFGWTLLIVGTSILLRELWGLTHGAT"
     gene            1138..>1543
                     /locus_tag="PL106_12495"
     CDS             1138..>1543
                     /locus_tag="PL106_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA33936.1"
                     /translation="MNVEQIYTGCLAQGAYYIESDGEAAIIDPLRETAPYIERAKAHG
                     TRIKYVLETHFHADFVSGHLDLARKTGATIVYGPNATTAFDAHIAKDGEELKLGKVTI
                     QVIHTPGHTMESTCYLLIGIDGKPHSLFTGDTL"
BASE COUNT          257 a          531 c          483 g          272 t
ORIGIN      
        1 acccggcggc ctcgccttcg aggaggccac gtacttgctg tcgcggctgg cggccagtgg
       61 gaggcgcatc atcggtttcg acctggtgga ggtgacgccc ggcaggaacg atgagtggga
      121 tgcgaacgtg ggcgcccgac tgctctggca cctctgcggt gtgatggcca atccctgagc
      181 caccaccacg catgcttgca tcacggccct ccggccaccc accggtacgt tcgtccatcg
      241 cggaacacgg ctgaacatgg agatcctcgg atacctctgt gccgcactca tgggcctggc
      301 cctgggcatg atcggcggtg gcggaagcat cctgaccgta cccatcctgg tatatctttt
      361 cggggtggat ccggtgcatg ccgcggcgta ttcgttgttc atcgtgggcc tcaccgcgct
      421 ggtgggcggc cttgggcacc tgcggatggg ccaggtggac caacggtccg tgttcgcctt
      481 cggcctgccc agcatattcg gcgtggcctt tacccggcgc ttcatcgttc ctgccctgcc
      541 cgacccattg ttgcaggtgg gcgcttggcg gatcgggctg gacccggtgc tgctcctgct
      601 cttcgccgtg ctcatgctgc tggccgcgcg gcaaatgctg cgcgcggcgg agcccgccga
      661 tggagcccct tccgatgttc cagccaacgg cggaggtctc gcgctgcgcg gcctgctcac
      721 gggggtcgcc acgagtctgg tgggtgcggg cgggggcttc ctcatcgttc ccgcgctcat
      781 ggcgttcgca cgcttgccca tgaaaaaagc cgtgggcacc tcgctcacca tcatcgccct
      841 gaacggcctg atcggcgcgg ccagcgaccc gcacatggca cagcaccccg actggaccct
      901 gatcctggtg gtcgctggca tcgccatcgc gggcatcctg gtgggcagcg tgctggggca
      961 ccgcgtcccg aacgagcggc tgaagcccgc cttcggttgg accctgttga tcgtcggcac
     1021 gtccattctc ctgcgcgaac tttggggcct gacccacggt gccacatgat cccgaacagg
     1081 acgtccgcac ccgtcagcac cgaatataat accgaaaaca tgaacgccac cggcaccatg
     1141 aacgtcgaac agatctacac cggatgcctg gcacagggcg cctactacat cgagagcgac
     1201 ggcgaggccg ccatcatcga tccgctgcgc gagacggcgc cctacatcga gcgggccaag
     1261 gcacacggca cccggatcaa atacgtgctg gagacacatt tccacgccga cttcgtcagc
     1321 ggtcacctgg acctcgcccg caagaccggg gccacgatcg tctacggccc taacgccacc
     1381 acggccttcg atgcgcacat cgcgaaggac ggcgaggaat tgaagctggg caaggtgacg
     1441 atccaggtca tccacacccc cgggcacacc atggaaagca cctgctacct gttgatcggt
     1501 atcgacggca agccccacag cctgttcacg ggcgacacgc tct
//
LOCUS       DAOQOC010000860         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65094_length_1542_cov_4.527236, whole genome shotgun sequence.
ACCESSION   DAOQOC010000860 DAOQOC010000000
VERSION     DAOQOC010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65094_length_1542_cov_4.527236"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PL106_12500"
     CDS             complement(<1..288)
                     /locus_tag="PL106_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33937.1"
                     /translation="MAAAGGAGVGFGWEVTTGLAAAFVGAGAAFLTGAGLTATAFLGA
                     GAGFFATGLGAGFLGAGFAAFLATGFTTFFDAGFLAGVAFFTGLTAFFEAGL"
     gene            419..829
                     /locus_tag="PL106_12505"
     CDS             419..829
                     /locus_tag="PL106_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNA33938.1"
                     /translation="MVKVQVSTLESSGKDRYSDHELEEFRQIINVKLDEARKDYELLK
                     QTLANTDNNGTEDTSPSFKMIEDGSETLSREETAQLASRQEKFIKHLEDALLRIRNKT
                     YGICRVTGRLISKERLRLVPHATLSIEAKQQMSN"
     gene            855..>1542
                     /locus_tag="PL106_12510"
     CDS             855..>1542
                     /locus_tag="PL106_12510"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNA33939.1"
                     /translation="MKRALLVIVLVLLTDQVLKVWVKTHFFLGEYIPMFGDNSSWAYL
                     QFVENKGMAFGLEFGGEAGKLLLTLFRMVAVVAIGWVLRWMVREKQSGTRIISLALIF
                     AGALGNIIDSAFYGLIFSASDPVTRNVAVIFPEGGGYAPFLHGAVVDMFYFPLWQGRF
                     PDWMPVWGGEPFEFFRPVFNIADAAISVGVALMILFQRKDKPDQPEPLRDQPGTPAHP
                     QEAQADPEHPN"
BASE COUNT          354 a          509 c          448 g          231 t
ORIGIN      
        1 caaaccggct tcgaagaagg ccgtcaagcc cgtaaaaaag gccactccag ccaaaaagcc
       61 ggcatcgaaa aaggtagtga agcccgtggc caaaaaagct gcgaagcccg cccccaaaaa
      121 accggctccc aagccggtgg cgaaaaagcc cgcaccagca cctaaaaaag cggtcgccgt
      181 caagccagca ccggtcaaga aagccgcccc ggcccccacg aaggccgcag ctaagcctgt
      241 ggtgacctcc cagccgaagc caacgcctgc tcctccggcg gcggccaaga aggccgcagc
      301 ggccaagtcg gcatccgccc cgaaaaagtc cgagaagcac gtgaaagaag agaaaagcgc
      361 cgccccgaag cccctcgttc agcagacctc cgccccggtg atgcccaaag ctaccccgat
      421 ggtgaaggtg caggtaagca cgctcgagag ttccggcaag gatcggtaca gcgatcatga
      481 attggaggag ttccgtcaga tcatcaacgt gaaattggat gaggcccgca aggattacga
      541 actgctgaag cagaccctgg ccaacacgga caacaacggc acggaggaca ccagcccatc
      601 cttcaagatg atcgaggacg gcagcgagac cctgagccgt gaggagaccg cccaactggc
      661 atcccgtcag gaaaagttca tcaagcacct ggaggacgcg ctcctgcgta tccgcaacaa
      721 gacctacggc atctgccgcg tcaccggtcg cctgatcagc aaggaacgcc tgcgcctggt
      781 accgcacgcc accctcagca tcgaggccaa acagcagatg agcaactgag cggagcgcca
      841 cgttccgcac cggtgtgaag cgcgccctgc tcgtcatcgt acttgtcctg ctgaccgacc
      901 aggtcctgaa ggtctgggtg aaaacgcatt tcttcctggg cgaatacata ccaatgttcg
      961 gggataacag ttcctgggcc tacctgcagt tcgtcgagaa caagggcatg gccttcggat
     1021 tggagttcgg gggcgaggcc ggcaaactcc tgctcacgct cttccgcatg gtggccgtgg
     1081 tggccatcgg ctgggtgctc cgctggatgg tgcgcgagaa gcagagcggc acgcgtatca
     1141 tcagcctcgc gctcatcttc gccggagcgc tgggcaacat catcgacagc gccttctacg
     1201 gcctcatttt cagcgcgagc gatcccgtga cgcgcaacgt ggcggtaatc ttccccgagg
     1261 gggggggata tgcgcctttc ctgcacggcg ccgtggtgga catgttctac ttccccctct
     1321 ggcaagggcg ctttcccgac tggatgcccg tctggggcgg agaacccttc gaattcttcc
     1381 ggccggtctt caacatcgcc gatgccgcca tcagtgtggg cgttgcgctc atgatccttt
     1441 tccagcgcaa ggacaaaccc gaccagcccg aaccgttgcg cgaccaaccg gggaccccgg
     1501 cccacccgca ggaagcccaa gccgacccgg agcacccgaa cg
//
LOCUS       DAOQOC010000861         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65099_length_1542_cov_3.927371, whole genome shotgun sequence.
ACCESSION   DAOQOC010000861 DAOQOC010000000
VERSION     DAOQOC010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65099_length_1542_cov_3.927371"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1444)
                     /locus_tag="PL106_12515"
     CDS             complement(152..1444)
                     /locus_tag="PL106_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA33940.1"
                     /translation="MRTAITFLAAALVLPLTAQLLTAEQAVQVALEHNHGIRLAKLDA
                     RTSELMNNIGNAGMLPTVDANGVYSLDNSSTKQTFFSGEVREASNANSRVLDGALALN
                     WTVFDGLAMFAAKDRLEALELIGKTGLRQRMESTVYDVLTNYYLAVQLNSAVAVQQEG
                     LHTSRERLTIARTAHGIGSGSGLQVVQARLDLSADSAALLGLMQQRTIAFNRLNTLLG
                     RAADTPAEVGTTIPAPEDLDLATVQKDARAGNCAVQQARQQQLLADVSVKELRGALFP
                     RIDLFGNYGYTKSTSAVGFLKSNQALGPDYGVRVNVPLFRGLQANRALEVAKITREQA
                     SVSTEQAQLQLEQQVLDGWSDYSTARQRVLLEETDLAGIRTQVGVALESYRIGVITSV
                     ELRDVQQGLIAAENRLLLAQYEAKSAELRLKWLAGRLL"
BASE COUNT          239 a          480 c          508 g          315 t
ORIGIN      
        1 tgcccatgtg caggtggggt tttggtggtt ttccgtctta ggacgaagat cgggtgagcc
       61 ggtcacatcg cgggcgaagg taaaatccgg caccggcaaa gcgatacggt atgggatcat
      121 gcacatgctc caggtgcagc gtgtccgtgg ctcacagcag tcgtccggcc agccacttca
      181 gccgcagttc cgccgacttg gcctcatact gcgccagcag caggcggttc tccgcggcga
      241 taaggccctg ttgcacatcg cggagctcca ccgatgtgat cacgcctatg cggtagcttt
      301 cgagcgccac gcccacctgc gtgcggatac ccgcgaggtc ggtctcctcc aacagcaccc
      361 gctgccgggc cgtggagtag tccgaccagc catcgagcac ctgttgttcc aactggagtt
      421 gtgcctgttc ggtgctgacg ctggcttgtt cacgggtgat cttcgccact tccagggcgc
      481 ggttggcctg caggccgcgg aacagcggca cattcacgcg aacgccgtag tccggtccaa
      541 gtgcttggtt gcttttcagg aagcccaccg cactggtgct tttggtgtag ccgtagttgc
      601 cgaagaggtc gatgcgcgga aagagcgccc cgcgcaattc cttcacgctc acatcggcca
      661 gcagttgttg ttgccgggct tgctggaccg cacagttgcc cgctcgggcg tccttctgca
      721 cggtggcgag gtcgagatcc tccggggcgg ggatcgtggt ccccacttcc gccggggtgt
      781 cggcagcccg gcccagcagc gtgttcaacc ggttgaaggc gatggtccgt tgttgcatca
      841 ggccgagcag tgcggcactg tcggcactga gatccagtcg ggcctgcacc acctgcaacc
      901 cgctgcccga cccgatgcca tgggcggttc gcgcgatggt gagtcgttcg cgcgaggtgt
      961 gcagcccttc ttgctgcacg gccacggcgc tgttcagttg cacggccagg tagtaattcg
     1021 tcagcacatc gtacacggtg ctctccatcc gctggcgcag tccggtcttg ccgatcagtt
     1081 ccagggcctc cagccggtcc ttcgcggcga acatggccag accgtcgaac acggtccaat
     1141 tcagggccag cgcaccatcc agcacgcggc tgttggcgtt ggaggcctcg cgcacctcgc
     1201 cgctgaagaa ggtctgcttg gtgctgctgt tgtcgaggct gtatacgccg ttggcatcaa
     1261 ccgttggcag catgccggcg ttgccgatgt tgttcatcaa ttcactggtg cgcgcgtcca
     1321 gtttcgccag ccggatgccg tggttgtgtt ccagcgcgac ctgcacggcc tgctccgccg
     1381 tgaggagctg ggcggtcaag ggcagcacca aggcggctgc gaggaaggtg atggcggtcc
     1441 tcatacgtca agcgatggtg ttggaggagg cggttccccg ggcgtctctt ccttgggcga
     1501 gtcggaaccg ctggccatgt agctgtacag ggcgggcaca ac
//
LOCUS       DAOQOC010000862         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65142_length_1542_cov_2.316745, whole genome shotgun sequence.
ACCESSION   DAOQOC010000862 DAOQOC010000000
VERSION     DAOQOC010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65142_length_1542_cov_2.316745"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PL106_12520"
     CDS             <1..814
                     /locus_tag="PL106_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNA33941.1"
                     /translation="VTLIDIAQASLDKGLGTIGKNLDRQVSKGAITEEQKAATLKNIA
                     TSTNLATGVKQAELVVEAATENVDLKLNIFRDLDAHAPANAILASNTSSISITRIATV
                     TKRPQQVIGMHFMNPVPVMKLVEVIRGYDTTDAVTRTVTDLSTAIGKIPVTVNDYAGF
                     IANRILMPMINESIEALFQGVGGVAEIDAIMKLGMAHPMGPLQLADFIGLDTCLAILR
                     VLHEGFGNPKYAPCALLVQMVTAGKLGVKSGEGFYVYTPGSKDLVVAPSFAR"
     gene            811..1329
                     /locus_tag="PL106_12525"
     CDS             811..1329
                     /locus_tag="PL106_12525"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA33942.1"
                     /translation="MNIRPAGLADIPLIRSIAHATWPMCYAGIITPEQIAYMLGRMYS
                     TKVLDDQLMNGHHFLLSEHHGTATGFAGFEHGYRPARTRLHKLYVLPDAQGTGAGRAL
                     LEAVFGAAVSAGDQGIELNVNKRNPAVGFYRKNGFSIERDEVLDIGSGFVMDDHVMLR
                     PLVGCPAAAVDP"
BASE COUNT          325 a          494 c          466 g          257 t
ORIGIN      
        1 cgttacactc atcgatatcg cccaggccag tcttgacaaa ggtctgggca ccatcggcaa
       61 gaacctggac cgccaggtgt ccaaaggcgc gatcaccgaa gagcagaaag ccgccacctt
      121 gaagaacatc gccaccagca ccaacctggc cacaggggtg aaacaagccg aattggtggt
      181 ggaagcggcc accgagaacg tggacctcaa gctcaacatc ttccgcgatc tggacgccca
      241 tgcccccgcg aacgcgatcc tggccagcaa caccagcagc atcagcatca cgcggatcgc
      301 cacggtaacc aagcggcccc agcaggtgat cggcatgcac ttcatgaatc ctgtcccggt
      361 gatgaaactg gtggaggtga tccgcggata tgataccacc gatgcggtta cacgtacggt
      421 gacggacctg agcaccgcga tcggcaagat ccccgttacg gtgaacgact acgcgggttt
      481 catcgccaac cggatcctga tgcccatgat caacgagagc atcgaggcgc tgttccaagg
      541 cgtgggcggt gtggccgaga tcgacgcgat catgaagctg ggcatggcgc acccgatggg
      601 cccgctgcaa ctggccgact tcatcgggtt ggacacctgt ctggccatcc tgcgcgtgct
      661 gcacgagggc ttcggcaacc ccaaatacgc gccctgtgcc cttttggtgc agatggtgac
      721 ggccggcaaa ctgggggtga agagcggcga ggggttctac gtgtacaccc cgggcagcaa
      781 ggacctggtg gttgccccgt cgttcgcccg gtgaacatcc gacccgcggg tcttgccgac
      841 atcccgctga tccggtccat cgcgcacgcc acatggccga tgtgctacgc gggcatcatc
      901 acgccagagc agatcgcgta catgttgggg cgtatgtaca gcacgaaggt gttggacgac
      961 cagttgatga atggccatca tttcctcctt tcggagcacc acggcacagc gaccggcttc
     1021 gcgggtttcg agcacggata ccgaccggcg cgcacgaggc ttcacaagct gtacgttctt
     1081 cccgatgcgc agggcaccgg ggcgggccgg gcgctgctcg aagccgtatt tggcgcggcc
     1141 gttagcgcag gcgaccaggg gatcgaactg aatgtgaaca agcggaaccc ggcggtcggc
     1201 ttctaccgaa aaaacggttt ctcgatcgaa cgggacgaag tgctcgacat cgggagcggc
     1261 ttcgtaatgg acgaccacgt gatgttgagg ccattggtcg gttgtccggc agcagccgtt
     1321 gatccgtgat ccgggcagcc gacagcagcc tctggtcatc cgcggacttc agcccgcacg
     1381 cacacatccg cacccccacc cttactggca ctctcaccgc accgaagcct gcgctcctcc
     1441 ctcccccgcg aaaagttcgg ccgccaatcg ggcatcgtga ccgtacacat cccggcggaa
     1501 agagacgatc ccatcgcgat cgacccaagc ggtgaagtaa cc
//
LOCUS       DAOQOC010000863         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65356_length_1539_cov_5.167116, whole genome shotgun sequence.
ACCESSION   DAOQOC010000863 DAOQOC010000000
VERSION     DAOQOC010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65356_length_1539_cov_5.167116"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PL106_12530"
     CDS             complement(<1..552)
                     /locus_tag="PL106_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA33943.1"
                     /translation="MRTQLRSFLFLASLAGATAVSAQIAVGGRVGANYAIESMRIQPD
                     PKDPPTNPKGLGMVFGAYVEIPFSDMVGLRPELGFSFRRIKMEATQNKDYNNEQVIMN
                     TDQGQQQGALTAKEEQKSVTDQRLQYFQINVPIMITPAEGFRFMVGPSFNFLMGGKQT
                     TDDTYTLKGTFTPNGGQQQQVNVD"
     gene            complement(702..>1539)
                     /locus_tag="PL106_12535"
     CDS             complement(702..>1539)
                     /locus_tag="PL106_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNA33944.1"
                     /translation="SGKLTFDPVHDTLTNDKGEQVKLAEPTGWELPPKGFAVEDAGYQ
                     APAADGSKVNVVVKPDSQRLQLLEPFPAWNGKNITGAVLLIKAKGKCTTDHISMAGPW
                     LRYRGHLDNISNNTLIGAINAFNGEADKVKNQLTGEYGAVPATQRAYKAAGVTSIVVG
                     DQNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLALTFSNEADYEKIQ
                     EDDRIDFTDLTAFAPGKPLTMVLNHKDGSKDTIQVNHTYNDQQIEWFKAGSALNLIRS
                     QQ"
BASE COUNT          228 a          452 c          502 g          357 t
ORIGIN      
        1 gtcgacgttc acctgctgct gctggccgcc gttgggtgtg aaggtgcctt tcagggtgta
       61 cgtgtcatcg gtggtctgct tgccgcccat gaggaagttg aacgaggggc ccaccatgaa
      121 gcggaaaccc tcggccggcg tgatcatgat cggcacgttg atctggaagt actgcaggcg
      181 ctggtccgtg accgacttct gttcctcttt cgcggtgagt gccccctgtt gctggccctg
      241 atccgtgttc atgatcactt gttcattgtt gtagtccttg ttctgcgtgg cctccatctt
      301 gatgcggcgg aaggagaaac ccagctccgg tcgcagaccg accatgtccg agaacgggat
      361 ctccacatag gcgccgaaga ccatgcccag ccccttgggg ttggtgggcg gatccttcgg
      421 gtcgggttgt atgcgcatgg attcgatggc atagttggcg cccacccggc caccaacggc
      481 gatctgggcg gaaactgcgg tagctcctgc cagggaggcg aggaacagga aggaacgtag
      541 ctgtgttcgc atgatggtcg ttcagttcag atactggcaa acctaacgat cctcggctga
      601 accgggcgca cgcctcccca taattcggga gagcgacggt tcgaccgtcc gggtgcgctc
      661 ggtcaccggt acgctaccca gccgacccgt ccggccccgt actactgctg cgatcggatc
      721 agattgagcg cactgcccgc cttgaaccac tcgatctgct ggtcgttgta ggtgtggttc
      781 acctggatgg tgtccttcga cccgtccttg tggttgagca ccatagtgag cggcttgccg
      841 ggcgcgaagg ccgtgaggtc cgtgaagtcg atgcggtcgt cctcctggat cttttcgtag
      901 tcggcctcgt tgctgaaggt gagggcgagc atcccttgct tcttcaggtt ggtctcgtgg
      961 atgcgggcga agctcttcac gagcacggcg gccacaccca agtggcgcgg ttgcatggcg
     1021 gcgtgttcgc ggctgctgcc ttcgccgtag ttctggtcgc ccaccacgat gctggtgacc
     1081 ccggcggcct tgtacgcgcg ttgcgttgcg ggcacggcgc cgtattcacc cgtcagctgg
     1141 ttcttcacct tgtcggcctc gccgttgaag gcattgatgg caccgatgag cgtgttgttg
     1201 ctgatgttgt ccaagtggcc ccggtagcgc aaccaagggc cggccatgct gatgtggtcg
     1261 gtggtacact tccctttggc tttgatgagc agcacggcac cggtgatgtt cttgccgttc
     1321 catgcgggga agggctcgag cagttgcaga cgctggctgt cgggcttcac caccacgttc
     1381 accttgctgc cgtcggcggc gggcgcctgg tagccggcgt cttccacggc gaagcctttg
     1441 gggggcagtt cccagcctgt cggttcggcc agcttcacct gctcaccctt gtcgttggtc
     1501 aacgtgtcgt gtaccggatc gaaggtaagt ttgccgctg
//
LOCUS       DAOQOC010000864         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65409_length_1539_cov_2.676550, whole genome shotgun sequence.
ACCESSION   DAOQOC010000864 DAOQOC010000000
VERSION     DAOQOC010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65409_length_1539_cov_2.676550"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PL106_12540"
     CDS             <1..912
                     /locus_tag="PL106_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNA33945.1"
                     /translation="ASKGNEKIGSTLKGIGPTYMDKTGRNGFRVGDLERSDRDNRYAA
                     LVKKHEHLLSLYSTTIAWKEAEKEWQDAASRLRDLRIVDSEHWLDERLRAGDRVLAEG
                     AQGTLLDIDFGTYPFVTSSSTISAGACTGLGIGPGSIGKVIGIFKAYSTRVGSGPFPT
                     ELMDATGEHIRQAGREFGSTTGRPRRCGWLDLPALRYAVMINGVTELAMMKADVLCGM
                     DRVKLCNSYRVNGIVTDRVPYDLSTGVEPVYETMPGWGDISSELPPALNAYVEHIERA
                     VGVPVRIVSTGPDRAQTLLREGVGAAH"
     gene            924..>1539
                     /locus_tag="PL106_12545"
     CDS             924..>1539
                     /locus_tag="PL106_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33946.1"
                     /translation="MTRRSNRSGADVPPFVPHTLTFLSPEDRNALEDALRKTSPISIR
                     KNPRKFGGPEGTAIPWCDTGLYLSERPSFTLDPLLHAGAYYVQEASSMLLEQAWLRTG
                     LNNENILALDLCAAPGGKSTHLASLLSDGSLLVCNEVMPGRQAALMENLWKQGRLNTM
                     ITGGRAQDLAAMEATFDLILVDAPCSGEGMFRKDPFARQQWNEGL"
BASE COUNT          309 a          486 c          489 g          255 t
ORIGIN      
        1 gcctcgaaag ggaacgagaa gatcggcagt acgctcaagg gcatcggacc gacgtacatg
       61 gacaagaccg gccgcaatgg tttccgcgtg ggtgatctgg aacgttccga tcgggataac
      121 cgctatgcgg cgctggtgaa gaaacacgag catctgctca gcctgtacag caccaccatc
      181 gcatggaagg aggccgaaaa ggagtggcag gacgccgcat cgcgcttgcg tgatctccgg
      241 atcgtggaca gtgaacattg gctcgatgaa cgcctgcggg ccggggacag agtactggcc
      301 gaaggggccc agggcacgtt gctcgatatc gatttcggca cctatccttt cgtcaccagc
      361 agcagcacga tcagcgcagg ggcatgtacg ggcctgggca tcgggccggg cagcatcggc
      421 aaggtcatcg gcatcttcaa agcatacagt acccgcgtgg gcagcggccc cttccccacc
      481 gaactcatgg atgccaccgg cgagcacatc cgccaggccg gccgcgaatt cggcagtacc
      541 accggccggc cgcgccgttg cggttggctg gacctgcccg ccctgcgcta cgccgtgatg
      601 atcaacgggg tgaccgaact ggccatgatg aaagccgacg tactgtgcgg catggaccgg
      661 gtgaagctgt gcaacagcta ccgcgtgaac ggtatcgtaa cggaccgggt cccgtacgac
      721 ctgagcaccg gcgtggagcc cgtttacgag accatgcccg gctgggggga catcagcagc
      781 gaacttcccc ctgccctcaa tgcctatgtg gagcacatcg agcgggcggt gggtgttcct
      841 gttcgcatcg tgagcaccgg acctgatcgc gcgcagacgc ttcttcgcga aggcgtagga
      901 gccgcgcact gaccggacac cccgtgacga ggcggagcaa ccggagcgga gcggacgtcc
      961 cgcccttcgt tccgcacacg ctcaccttcc tttcgcccga ggatcggaac gccttggagg
     1021 atgcacttcg caaaacaagt ccgataagca tccggaagaa tccgcgcaaa ttcggtggcc
     1081 ccgaggggac ggctattccg tggtgcgaca cgggccttta cctctccgag cgcccctcct
     1141 tcacgctgga cccccttctg cacgccggtg cgtactatgt tcaggaagca tcgagcatgc
     1201 tgctcgaaca ggcctggctc cgcaccggtc tgaacaacga gaacatcctg gcgctggacc
     1261 tctgtgccgc accgggcgga aaatccaccc atctcgcttc gctgctcagc gatggctcgc
     1321 tcctggtgtg caacgaagtg atgcccggcc gccaggcggc actgatggag aacctgtgga
     1381 agcagggccg cctgaacacc atgatcaccg gaggacgggc gcaggatctc gcggcgatgg
     1441 aagccacgtt cgatctgatc ctggtggatg ctccgtgctc cggtgagggc atgttccgaa
     1501 aagacccctt cgcccggcaa caatggaacg aaggtcttg
//
LOCUS       DAOQOC010000865         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65415_length_1539_cov_2.553908, whole genome shotgun sequence.
ACCESSION   DAOQOC010000865 DAOQOC010000000
VERSION     DAOQOC010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65415_length_1539_cov_2.553908"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PL106_12550"
     CDS             complement(<1..687)
                     /locus_tag="PL106_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA33947.1"
                     /translation="MHTLLPFLLAMVAAVVLLHMLAGKLRVAYPIILVVAGLVVSFIP
                     GLPRMRVDPDLIFFLVLPPLLFEAAWSISWKELWRWRRVITSFAFLVVFFTAMAVAVV
                     TSQVIPGFTLALGFLLGGIVSPPDAVSVGAVTRFVRIPRTTKALLEGESLLNDASALI
                     IFRFALIAVGTGQFIWQQAAVDFAWMVAGGVGTGLLLGWFFVQLHRRLPTEAASDIAL
                     TLIEPYFMYWA"
     gene            complement(754..1101)
                     /locus_tag="PL106_12555"
     CDS             complement(754..1101)
                     /locus_tag="PL106_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA33948.1"
                     /translation="MQTTALDHFYLQQPEPVKGCYLALRDIILAQSAHITPEWKYGCP
                     FFYYKGKMFCYLWFHKEHQQPYLAIMKGKSISDPLLLAEGRVNIRIMLFDPEKDLPVR
                     SIKRVLQKALELF"
     gene            complement(1264..>1539)
                     /locus_tag="PL106_12560"
     CDS             complement(1264..>1539)
                     /locus_tag="PL106_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33949.1"
                     /translation="LFVVLVPFSLIPEMLTVGHWAFWLSIPISTLIGLVFIIMELVGD
                     YNENPYMGTPNGMPMLSMCRSIEIDLRQMLGEQELPKPIAPVNGVLM"
BASE COUNT          328 a          464 c          471 g          276 t
ORIGIN      
        1 ggcccagtac atgaagtagg gttcgatcaa cgtgagcgcg atgtccgatg ccgcctccgt
       61 gggcagccgc cggtgcagct gcacgaagaa ccagcccagc agcaggccgg tgcccacgcc
      121 gcccgccacc atccaggcga agtccacagc ggcctgctgc cagatgaact ggccggtgcc
      181 cacggcgatc agcgcgaagc ggaagatgat cagcgcggag gcgtcgttca gcaggctctc
      241 cccttccagc agcgccttgg tggtgcgcgg gatcctcacg aagcgcgtta cggcgcccac
      301 gctcaccgca tcgggcgggc tcacgatgcc gcccagcagg aaacccagcg ccagcgtgaa
      361 gccggggatc acctggctgg taaccaccgc cacggccatc gcggtgaaga agaccaccag
      421 gaaggcgaag ctggtgatca cgcgccgcca gcgccacagc tccttccacg agatggacca
      481 ggccgcctcg aacagcagcg gcggcagcac caggaagaag atcaggtccg gatccacccg
      541 catgcgcggc agcccgggga tgaagctcac caccagccca gccaccacca gtatgatggg
      601 atacgccacg cgcagcttgc ccgccagcat gtgcaacagc accacggcgg ccaccatggc
      661 gagcaggaag ggaagcaatg tgtgcatggg tttgctgtgg atcgatcgga actgatcgcg
      721 agtatcaata tggtcagtgg tctcgacgcg tgcctaaaat aattccagcg ctttttgcag
      781 aacgcgcttg atgctccgaa caggcagatc cttttcggga tcgaacaaca tgatccggat
      841 gttcaccctg ccttcggcca gcaacaaggg atcgcttatg gacttgcctt tcatgatggc
      901 gaggtagggt tgttggtgct ccttgtggaa ccacaggtag cagaacatct tccccttgta
      961 gtagaagaac ggacaaccgt atttccactc gggcgtgatg tgtgcgctct gcgccaggat
     1021 gatgtcccgc aaggccaggt agcagccttt caccggttcg ggttgctgga ggtagaaatg
     1081 atccagcgcc gtggtttgca tcgttgcgtt aagctggctc tactgtgccg acgacgaaga
     1141 tccggttcgg tcagtcgcga tgcgctttgt aaagaggtga ggaagtgagg acgttcacgg
     1201 ctatcacttc ctctcttcct cacctcctca cggatcggtg tgatcgaccg cctcgtacgt
     1261 ccttcacatc aacacaccgt tcaccggtgc aatgggcttg ggcagctctt gttcgccgag
     1321 catttggcgc aggtcgatct cgatgctgcg gcacatcgag agcatcggca taccgttcgg
     1381 ggtgcccatg tacgggttct cgttgtagtc gcccaccaat tccatgatga tgaagaccag
     1441 cccgatgagg gtggaaatgg gaatggacaa ccagaaggcc caatggccca cggtgagcat
     1501 ctcggggatc aggctgaaag gcaccaggac cacgaagag
//
LOCUS       DAOQOC010000866         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65471_length_1538_cov_3.828726, whole genome shotgun sequence.
ACCESSION   DAOQOC010000866 DAOQOC010000000
VERSION     DAOQOC010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65471_length_1538_cov_3.828726"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>1538
                     /locus_tag="PL106_12565"
     CDS             22..>1538
                     /locus_tag="PL106_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33950.1"
                     /translation="MYDHVLGGTEQLAGLTVMGTGTSLFMRMTSDLSVSCASGSMTSW
                     VWTVDCLDCETATATAGDVAIDCDAQSFTVPVNLMDMGSADTVLITNDAGLPPVEVTA
                     AGSYVAGPFLAGVPVALGVMNAENVLCAAPLGTFLGTVCATPVDCEAPLQEHTYCYGE
                     YDTEQWLYQSSGASPLGIVFSAGTIENAAYDHLTIYDGPDQAAPVLYDHVLNSTEQLA
                     GLTVAGTGNVLYMRMTSDYSVSCSSGAMTPWVWTVGCISCTDPQATFGTVADCPHNRY
                     NVDVNVTGLGSGDDVLITDSWSGDTLSGIGLGNTVIGPIPMGVGVQVSVINGSNPFCS
                     TVSEQLALPAAACVITACDLLQVEHCYADADTVWFIYTSGESVPVTIFFNSGQLLPGD
                     QIRVFNGLTIDAQLVYAGNFGGDISGYSLTSNNPDNALLVQVVSDGTGSCATGEASPA
                     LHWSVGCGFTGLDLAGGTAVGLFPNPSTGMVQVMVPWPGIEVVRAEVFDPAGRKV"
BASE COUNT          269 a          456 c          511 g          302 t
ORIGIN      
        1 accgatcagg cggcgccgat attgtacgac cacgtgcttg gtggtaccga acaattggca
       61 ggcctcacgg tgatgggcac aggcacgtcc ttgttcatgc gcatgacctc cgacctcagc
      121 gtatcgtgtg ccagtggtag tatgacatcc tgggtgtgga cggtggactg cctggattgc
      181 gaaacggcca cggccacggc cggggatgtg gcgatcgatt gcgacgcgca gagcttcacg
      241 gtcccggtca acctcatgga tatgggttcc gcggacacgg tgctgatcac caacgatgcg
      301 ggtcttccac cagtggaggt gacggctgcg ggctcgtacg tggcaggtcc gttcctcgcg
      361 ggcgtccccg ttgcccttgg ggtaatgaac gccgagaacg tcctttgtgc cgcgccgctg
      421 ggtaccttcc tgggcaccgt gtgcgccacg cccgtggatt gtgaggcccc gctgcaggaa
      481 catacatact gctacggtga atacgatacc gaacaatggc tctaccagag ttcgggcgca
      541 tcaccgctgg gaatcgtgtt ctccgcgggc accatcgaga acgcggcgta cgaccatctg
      601 accatctacg acggccccga tcaggccgcc ccggtgttgt acgatcacgt cctcaacagc
      661 accgaacaac tggccgggct caccgtggca ggcacgggca acgtgctcta catgcggatg
      721 acctcggact acagtgtgtc ctgctccagt ggggccatga cgccctgggt gtggacggtg
      781 ggttgcatca gttgcacgga tccgcaggcc acgttcggaa cggtcgccga ttgtccgcac
      841 aaccggtaca acgttgatgt gaacgtgacc ggcctgggtt caggggacga cgtgctcatc
      901 accgactcat ggagcgggga cacgctcagc ggcatcggct tgggcaacac ggtcatcggt
      961 cccattccca tgggcgtcgg ggtgcaggtg tcggtgatca atggatccaa tccgttctgc
     1021 agtacggtga gtgagcagct cgcattgccc gctgcggcct gtgtgatcac ggcttgcgac
     1081 ctgctgcagg tggagcattg ttacgccgat gcggacacgg tctggttcat ctacacgagt
     1141 ggcgagagcg tcccggtgac catcttcttc aacagtgggc aactgctgcc cggggaccag
     1201 atccgcgtct tcaacggact gaccatcgac gcccaactgg tgtacgcggg gaatttcggt
     1261 ggcgacattt cggggtattc cctcacgagc aacaacccgg acaatgcgtt gctggttcag
     1321 gtcgtgagcg acggaacggg ttcttgcgcc acgggggagg cgtcccccgc actgcactgg
     1381 tcggtcggct gtggattcac cgggctggac ctggcgggtg gaacggccgt tgggctcttc
     1441 ccgaatccca gcaccggtat ggtccaggtc atggtgccat ggccgggcat cgaggtggtg
     1501 cgggccgagg tgttcgaccc ggccggtcga aaggtgca
//
LOCUS       DAOQOC010000867         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_65591_length_1537_cov_2.618084, whole genome shotgun sequence.
ACCESSION   DAOQOC010000867 DAOQOC010000000
VERSION     DAOQOC010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65591_length_1537_cov_2.618084"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PL106_12570"
     CDS             <1..780
                     /locus_tag="PL106_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33951.1"
                     /translation="GTIGDIGVEVMSGCSGTSILCATGTTEYMVDVEPDMGYVVRVFS
                     NNDLGLGGTFDICVSIPTGACDAGAPVTLEGDTSVVICVGEGPIVLADDPAGEGSVFV
                     VTDPSGTIVLFADSSQVEVDGLSSGTYQVWSVAFAGELGGAVPGQPLSGLTTTGDCLD
                     VSDASLEVVIEVCTAVHEQMGTPLVRMDNDHALIWQGTSQHIDLRLHDTRGALVFHQL
                     LSAVQGQVLLNTSQIGLCPGIYAVCVGSGDTTTCCNRVFVH"
     gene            945..>1537
                     /locus_tag="PL106_12575"
     CDS             945..>1537
                     /locus_tag="PL106_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33952.1"
                     /translation="MRTHSALLLLTAGALYLAPDRAAAQPANDLCTNTTIQALSVPGT
                     VTVTGDNTGGLDNDGLGWEAVWEAFTLTSCADVTVDFCGSDPAYVEGDWFMLLYRDCP
                     PLTEFWNNGQEQWTCPDQNLTMYFDGLDPGTYYYPVYAGGGNVGPYTINFKATACQDT
                     VNQAPDAGDDVFTMDQDSTLEASVADNDSDVDNDSTEL"
BASE COUNT          294 a          454 c          489 g          300 t
ORIGIN      
        1 ggcaccatcg gcgatatcgg ggtggaagtg atgagcggct gctcgggcac gtccattctc
       61 tgcgccacgg ggaccaccga gtacatggtg gacgtggagc cggacatggg ctacgtggtg
      121 cgggtcttca gcaacaacga cctcggcctc ggcggcacgt tcgacatctg tgtgagcatt
      181 ccgaccggtg catgtgatgc gggggccccg gtgaccttgg agggggacac gtccgtggtc
      241 atctgcgtgg gcgaggggcc gatcgtgctc gcggatgatc cggctggcga aggctcggtc
      301 ttcgtggtga ctgatccctc gggcaccatc gttctattcg ctgacagcag ccaggtcgag
      361 gtggatggac tttcgtccgg gacataccag gtctggagcg ttgcgttcgc gggtgagctt
      421 ggtggtgcag tgccaggcca gcccttgtca gggctgacga ccacaggcga ctgcctggat
      481 gtgagtgatg cgtcgttgga ggtggtcatc gaggtgtgta ccgccgtcca tgaacagatg
      541 ggaaccccgc ttgtgcgcat ggacaatgac catgcactga tctggcaagg gacttcgcag
      601 catatcgacc ttcgtctgca cgatacccgt ggggcgctcg tgttccacca gctcctgtcg
      661 gccgtgcagg gtcaagtctt gctcaacacg tcgcagatcg gcctgtgccc gggcatctat
      721 gcggtgtgcg tgggatcggg tgacacgacc acctgctgca accgggtttt cgtccactga
      781 aaggagcgct acatgggccg aacacggcca cgtggataaa tgcggcggtt catggggtgt
      841 ccagcacgct cgggcatcca actttgcagt gctctttccg aacgtcggga ccaggtgcac
      901 ccacaccagc gttccgaacc gaacacaaca gaacaacctt gtccatgaga acacattccg
      961 cactcctcct gcttaccgct ggagccctgt atctggcacc ggaccgtgct gctgcccagc
     1021 cggccaacga tctctgcacc aatacgacca tacaagccct ctccgttccg ggcacggtta
     1081 cggtgaccgg ggacaacacg ggcggtttgg acaatgacgg tctcggttgg gaagccgtat
     1141 gggaggcctt cacgctcacc tcgtgcgccg atgtgacggt cgacttctgc ggcagtgatc
     1201 cagcctacgt ggaaggggat tggttcatgc tcctgtatcg ggattgccct cctttgaccg
     1261 aattctggaa caacggccaa gagcaatgga cctgcccgga ccagaacctg acgatgtatt
     1321 tcgacggact cgacccaggc acctattact atccggtgta cgccgggggc ggcaacgtgg
     1381 gaccctacac gatcaacttc aaagccaccg cgtgccagga caccgtgaac caggctccgg
     1441 atgccgggga tgatgtgttc accatggacc aggacagcac gctcgaagca tcggtcgctg
     1501 acaacgacag tgatgtggac aacgacagca ccgagct
//
LOCUS       DAOQOC010000868         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66037_length_1532_cov_2.002708, whole genome shotgun sequence.
ACCESSION   DAOQOC010000868 DAOQOC010000000
VERSION     DAOQOC010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66037_length_1532_cov_2.002708"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1190
                     /locus_tag="PL106_12580"
     CDS             75..1190
                     /locus_tag="PL106_12580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA33953.1"
                     /translation="MSGKARISILDGFRAVAIISVMLYHYFSRWIPPNVDVSLYPYGT
                     RYDYFKYGYLGVEFFFVISGFVIFFTLERTKTWSVFWQKRMIRLLPSMVIASFLTLAF
                     FSLFDRSGLFPASHDPKNLIPSVSFVHPGIFNHLFNLDLTYITGSYWSLWPEIQFYML
                     SSVIFFLDKKRFLMNFMIISIVLIALDRFILNAGGALGSSMGPPGFYTEFISNGFNLI
                     TYLPFFCLGILFYRISSDRIRGTRTSPFIAVCCTVIALHVVLSGDVFAVRMIYSVIIC
                     TFLVFIYFPRYLGPLGRTPMSEVGESSYFLYLIHENLGVFMIYALGRHIWPDSFLMPA
                     IVIASLSWLSIVYTRTVDMPVNNWLKTRLIGVRKDQG"
     gene            complement(1293..>1532)
                     /locus_tag="PL106_12585"
     CDS             complement(1293..>1532)
                     /locus_tag="PL106_12585"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNA33954.1"
                     /translation="GGLQEETTVLGVPCLTLRPNTERPVTITLGSNELVPLEMDAVHA
                     AITRIEQGTFKKGKIPPLWDGHATERIIEVLERVL"
BASE COUNT          288 a          450 c          407 g          387 t
ORIGIN      
        1 cccacctcgg ccagtcgtgc gaaccatcgc tgtacgacaa cccttcctcg tggccgacct
       61 gagcgaacga accgatgtcc ggtaaagcaa ggatctccat tctggacggg ttcagggcgg
      121 tggcgatcat ctcggtgatg ttgtaccatt acttctccag atggataccg cccaatgttg
      181 atgtatcctt atatccctac gggaccagat acgactattt caagtatggt tacctcggcg
      241 tggagttctt cttcgtcatc agtggcttcg tcatcttctt cacgctcgag cgaacgaaga
      301 cctggtccgt gttctggcag aagcggatga tccgattgct cccatccatg gtgatcgcat
      361 cgtttttgac gctggccttc ttctcgctgt tcgacaggag cggcctgttc cccgccagcc
      421 atgacccgaa gaacctcata cccagcgtgt cgttcgtgca tccggggata ttcaaccact
      481 tgttcaacct cgatctcacg tacatcacgg gctcgtactg gagtctctgg cccgagatcc
      541 agttctacat gctctccagt gtcatttttt ttctcgacaa gaagcggttc ctgatgaatt
      601 tcatgatcat ctccatcgtc ctgatcgcat tggaccgctt catcctcaac gcagggggcg
      661 ccttggggtc atccatgggg ccaccgggct tttacacgga gttcatctcc aacgggttca
      721 acctgatcac gtacctgcca ttcttctgcc tgggcatcct cttctaccgg atatcatcgg
      781 acaggatcag gggcaccagg acctccccgt tcatagccgt gtgttgcacc gtgatcgcgc
      841 tccatgtcgt actctccggc gacgtgttcg cggtcaggat gatctattcc gttataatat
      901 gtacgttcct ggtattcatt tattttccac ggtacctcgg tcctcttggg cgaacaccga
      961 tgagcgaggt cggggagtcg tcctatttcc tgtacttgat acatgagaac ctgggtgttt
     1021 tcatgatcta tgcactgggc cgccacatct ggcccgactc cttcctcatg ccggcgatcg
     1081 tgatcgcatc cctttcgtgg ctcagtattg tgtataccag gacggtggac atgcccgtga
     1141 acaattggct gaagacccgg ctcatcggcg tccggaagga tcaaggatga ccatgcatgg
     1201 atccgcgcag cttgaagacg cggaccggat tgtaagcttc accgaacttc ggccgggtga
     1261 aggtggagat cagatggatc ttcccagccg gatcacagca cgcgctccag aacttcaatg
     1321 atgcgctccg tagcgtgccc gtcccacagg ggcgggatct ttcccttctt gaaagtgcct
     1381 tgttcgatcc gggtgatggc agcgtgcaca gcgtccattt ccagtggaac gagctcgttg
     1441 ctgcccaggg tgatggtcac cggacgttcg gtattggggc gcagcgtgag gcagggcacg
     1501 ccgagcacgg tggtctcctc ctgcaagcca cc
//
LOCUS       DAOQOC010000869         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66200_length_1529_cov_4.307327, whole genome shotgun sequence.
ACCESSION   DAOQOC010000869 DAOQOC010000000
VERSION     DAOQOC010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66200_length_1529_cov_4.307327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PL106_12590"
     CDS             <1..310
                     /locus_tag="PL106_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNA33955.1"
                     /translation="EKMIRLSGKEPVRAGEQPGPGRIAIAITGLRPGEKRFEELLASA
                     ENTLPTHHPRILIGRVRDVDATFVRHGVDALIAAAGRGDREGCLVELRKLVPEYAPQA
                     "
     gene            371..778
                     /locus_tag="PL106_12595"
     CDS             371..778
                     /locus_tag="PL106_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33956.1"
                     /translation="MTRDKNLETAKLQKEIGDHIGVGPDSLVFNFEETGRRKRVKLFL
                     ITINPRHDQSFLFHSTEGSDRMDALREMLEYVRTYKERSSSFTVQWSARGQGTLQTSY
                     FRAKNVLEALDKLFYDRDPNSITVFSVIMNPIT"
     gene            856..1284
                     /locus_tag="PL106_12600"
     CDS             856..1284
                     /locus_tag="PL106_12600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA33957.1"
                     /translation="MVTTSIQIRFNDMDLAGHAHNGVYLTWFELGRMAFFRTFIEREH
                     DWKKQGMILARNEVDHRAPVHLNDQAEVDCWCDRIGNTSFDLRYRITVLKDGQRQLRA
                     EGRSVMVCFDYTTNSPIPVPAAWRTALARMMDTDKKTART"
     gene            1281..>1529
                     /locus_tag="PL106_12605"
     CDS             1281..>1529
                     /locus_tag="PL106_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HNA33958.1"
                     /translation="MKTTIKVLPAALLIIGLSACEGQQVLGTLEAIQNAGATTLSNDE
                     VVSGLKEALRTGTERSVSIGSALDGFNKNNLIRIPFPQD"
BASE COUNT          339 a          483 c          467 g          240 t
ORIGIN      
        1 cgagaagatg atccgcctga gcggcaagga gccggtgcgg gcgggcgagc aacctggccc
       61 ggggcggatc gccattgcca tcacggggct gcggcccggt gaaaaacgct tcgaggaact
      121 gctcgcttcg gccgagaaca cactgcccac gcaccatccg cgcatcctta ttggtcgcgt
      181 ccgtgacgtg gacgcgacgt tcgtgcggca cggcgtggat gcgctcatcg ccgctgccgg
      241 acggggcgac cgcgaagggt gcctggtcga gctgcgcaag ctggttcccg agtacgcacc
      301 gcaagcttga gggtcctgaa cgccgttgcg tcaacggcct accttcgccg cgatcaggta
      361 atgcaccaac atgacccgcg acaagaacct cgagacggcc aagttgcaga aggagatcgg
      421 cgaccacatc ggcgtggggc ccgacagcct ggtgttcaac ttcgaggaga cggggcgacg
      481 caagcgtgtg aagctcttcc tcatcaccat caatccgcgc cacgaccaga gcttcctctt
      541 ccacagcacc gagggcagcg accgcatgga cgccctgcgt gaaatgctgg aatatgtacg
      601 tacatacaag gagcgatcga gcagcttcac cgttcagtgg agcgcccgcg ggcaaggcac
      661 gctgcagacg tcgtacttcc gtgccaagaa cgtgctggag gccctcgaca agctttttta
      721 tgatcgcgac ccgaacagca tcacggtgtt ctctgtgatc atgaacccga tcacctgagc
      781 gcaggagcgg gccgcaagaa gctggcggtc ttggaacccc gggcgaacac ggacacatca
      841 aacacacgcg cacgcatggt caccacatcc atccagatcc gcttcaacga catggatctg
      901 gcgggacacg cgcacaacgg cgtgtacctc acctggttcg aattggggcg gatggcgttc
      961 ttccgcacgt tcatcgagcg ggagcacgac tggaagaaac agggcatgat cctggcacgc
     1021 aacgaggtgg accatcgcgc accggtgcat ctgaacgatc aggctgaagt ggactgttgg
     1081 tgcgaccgga tcggcaacac gagcttcgac ctgagataca ggataaccgt gctcaaggac
     1141 gggcaacggc aactgcgcgc cgaaggccgc agcgtgatgg tgtgcttcga ctacaccacc
     1201 aattccccca ttcccgtacc ggccgcctgg cgcaccgccc tggcacgaat gatggatacc
     1261 gacaagaaaa cagcccgaac atgaagacga ccatcaaggt actgcccgcc gcactcctga
     1321 tcatcggctt gagcgcctgc gaggggcagc aagtactcgg cacgctcgaa gccatccaga
     1381 acgcgggggc gaccacgctg agcaacgatg aagtggtgag cggattgaag gaggccttgc
     1441 gtaccggcac cgaacgcagt gtttccatcg gctccgcgct cgacggcttc aacaagaaca
     1501 acctgatccg tatccccttc ccgcaggac
//
LOCUS       DAOQOC010000870         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66243_length_1529_cov_2.823609, whole genome shotgun sequence.
ACCESSION   DAOQOC010000870 DAOQOC010000000
VERSION     DAOQOC010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66243_length_1529_cov_2.823609"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /gene="rplF"
                     /locus_tag="PL106_12610"
     CDS             complement(<1..253)
                     /gene="rplF"
                     /locus_tag="PL106_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNA33959.1"
                     /translation="MSRIGKAPIPVPNGVQVNVSDKNVVTVKGPKGTLTQTIDPAIKV
                     KTEGAELVFERPTDLKHHRAIHGLYRALVANMVKGVSEGF"
     gene            complement(269..667)
                     /gene="rpsH"
                     /locus_tag="PL106_12615"
     CDS             complement(269..667)
                     /gene="rpsH"
                     /locus_tag="PL106_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNA33960.1"
                     /translation="MTTDPIADYLTRVRNAILARKKVVEVPASGLKKDITRILYDKGY
                     ILSWKVEDDGKQGIIKIALKYDRSTRRNAIRELTRVSSPGLRQYAHSGELPRVLNGLG
                     VAIISTSKGVLTDKEARTLGVGGEVICYVS"
     gene            complement(711..980)
                     /gene="rpsN"
                     /locus_tag="PL106_12620"
     CDS             complement(711..980)
                     /gene="rpsN"
                     /locus_tag="PL106_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNA33961.1"
                     /translation="MAKESMKARERKRAKMVEKFAAKRAALKKAGEWDKLQQLPANSS
                     RVRKHNRCGISGRPKGYIRLFGISRNMFRQMALEGKIPGVTKSSW"
     gene            complement(990..>1529)
                     /gene="rplE"
                     /locus_tag="PL106_12625"
     CDS             complement(990..>1529)
                     /gene="rplE"
                     /locus_tag="PL106_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNA33962.1"
                     /translation="SPRLKTTYKEKVVPALRKEFNYKSSMQVPRLMKIAVNQGLGNAV
                     GDKKIVENAIGEMTMITGQKAVGTVSKKDISNFKLRKGMPIGARVTLRGDKMYEFLDR
                     LIAVALPRTRDFRGVAAKGFDGRGNFTFGVKEQIIFPEIDIDKTPRIQGMDITFVTSA
                     KTDQEAKALLKEFGFPFTK"
BASE COUNT          215 a          442 c          500 g          372 t
ORIGIN      
        1 cgaaaccctc gctcacgcct ttcaccatgt tggccacgag cgcacggtac aggccgtgaa
       61 tggcgcggtg gtgtttcagg tcggtggggc gttcgaacac gagttcggca ccttcggtct
      121 ttaccttgat ggcgggatcg atggtctggg tgagcgtacc cttgggacct ttcacggtca
      181 ccacgttctt gtcgctcacg ttcacctgca cgccgttggg tacagggatg ggggcttttc
      241 cgatgcggct catggtggtc ggtcgctgtt agctcacgta gcagatcact tcaccgccca
      301 cgcccagggt acgtgcctcc ttatcggtga gcacaccctt gctggtgctg atgatggcca
      361 cgccgaggcc gttgagcacg cgggggagct ccccgctgtg ggcgtactga cgcaggccgg
      421 gggagctcac gcgggtgagt tcgcggatgg cgttgcggcg cgtgctgcgg tcgtatttga
      481 gggcgatctt gatgatgccc tgcttgccgt cgtcctccac tttccacgaa aggatgtagc
      541 ccttgtcata gaggatgcgg gtgatgtcct tcttgaggcc gctggcgggg acctccacca
      601 ctttcttacg cgccaggatg gcgttgcgaa cgcgggtgag gtaatcggcg ataggatcgg
      661 tcgtcatgtt ctttactgtt gatgatgagg ggtcgacgtg aacgtcgccc ttaccagctg
      721 ctcttggtta cgcccgggat ctttccctcc agggccattt ggcggaacat gttccggctg
      781 atgccgaaga ggcggatata gcctttcgga cggccgctga tgccgcagcg gttgtgcttg
      841 cgcacgcggc tgctgttggc gggtagctgc tgcagcttgt cccactcgcc ggccttcttc
      901 agcgcggcgc gcttggcggc gaatttctcc accattttgg cacgcttccg ctcgcgggcc
      961 ttcatgcttt ccttggccat ctctcgtctt tatttggtga aggggaatcc gaattctttc
     1021 agcagggcct tggcttcctg gtccgtcttg gcgctggtca cgaaggtgat gtccatgccc
     1081 tggatgcgcg gggtcttgtc gatgtcgatc tccgggaaga tgatctgctc cttcacgccg
     1141 aaggtgaagt tgccccggcc atcgaaccct ttggcggcca caccgcggaa gtcgcgggta
     1201 cggggcaggg ccacggcgat caggcggtcg aggaactcgt acatcttgtc gccgcgcagt
     1261 gtgacgcggg caccgatggg catgcccttg cgcagtttga agttgctgat gtccttcttg
     1321 gagaccgtgc ccacggcctt ctggccggtg atcatggtca tctcgccgat cgcgttctcc
     1381 acgatcttct tgtcgcccac ggcgttgccg aggccctggt tcacggcgat cttcatcagg
     1441 cggggcacct gcatggagct cttgtagttg aactccttgc ggagggccgg aacgaccttc
     1501 tccttgtagg tggttttgag gcgggggct
//
LOCUS       DAOQOC010000871         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66327_length_1528_cov_2.670061, whole genome shotgun sequence.
ACCESSION   DAOQOC010000871 DAOQOC010000000
VERSION     DAOQOC010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66327_length_1528_cov_2.670061"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PL106_12630"
     CDS             <1..327
                     /locus_tag="PL106_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNA33963.1"
                     /translation="MLWQHGWPKLTGFSERMDHFGDPIGLGSTVSLGLITFAETACAL
                     LVALGLWTRVSTLPLIIGMAVAAFMSNGDGPFGQQELPVFYLVAFLAILLAGSGRFSL
                     DRLKFS"
     gene            complement(335..1105)
                     /locus_tag="PL106_12635"
     CDS             complement(335..1105)
                     /locus_tag="PL106_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA33964.1"
                     /translation="MTTTSARVLVVEDESIIRMDIENALKRAGHAVVASCASGEEAIE
                     LCAEHKPEVVLMDIALNGPLSGIDAAHVIHETAGSAVIFLTANSDVATVNRARTAMPY
                     GYVIKPFRPVDLSVAIEMAVHNHVQDKRVRRERDDLKMRLMGEGDQSALFITVKGRQQ
                     RILCRDIRFIQALKDYVGIQVQGRRHVVYSTMASMEQRLPKDKFLRVHRSYIVHVDAI
                     KAIEDHQIVMEDGEEKIPIGGLYLTTVREKLGLDGSLT"
     gene            complement(1259..1384)
                     /locus_tag="PL106_12640"
     CDS             complement(1259..1384)
                     /locus_tag="PL106_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33965.1"
                     /translation="MYRHNHSARYYWINGEAPVFSVGGARKRAKVKTGALAPLHT"
BASE COUNT          247 a          472 c          462 g          347 t
ORIGIN      
        1 atgctgtggc agcatggctg gcccaagctc acgggcttca gcgagcggat ggaccatttc
       61 ggtgatccca tcggattggg atcaaccgtc tcgttggggc tcatcacctt cgccgaaacg
      121 gcctgtgccc tgttggtggc gctcggcttg tggacccggg tctccaccct gccgctcatc
      181 atcggtatgg ccgtggccgc gttcatgtcg aatggcgacg ggcccttcgg ccagcaggag
      241 ctccccgtgt tctacctggt ggccttcttg gccatcctgc tcgccgggag cgggcgcttc
      301 agcctcgatc gcttgaagtt cagctgatcc ggggtcacgt gagcgagccg tcgagaccga
      361 gcttctcgcg taccgtggtc aggtagagcc ctccgatcgg gatcttctcc tcgccgtcct
      421 ccatcacgat ctggtggtcc tcgatggcct tgatcgcgtc cacgtgaacg atgtatgagc
      481 gatgcacgcg caggaacttg tccttgggca ggcgttgttc catgctggcc atggtgctgt
      541 acaccacgtg gcgcctgcct tgcacctgga tgcccacgta gtccttcagc gcttggatga
      601 accggatatc gcggcaaaga atacgttgct ggcggccctt cacggtgatg aacaaggcgc
      661 tctgatcgcc ttcgcccatc aggcgcatct tcaggtcgtc ccgctccctc ctgacccgct
      721 tgtcctgcac atggttatga accgccatct cgatggccac gctcagatcc accggccgga
      781 acggtttgat cacgtaaccg tacggcatcg cagtgcgtgc gcggttcacc gtggccacgt
      841 cggagttggc cgtcaggaag atcaccgcgc ttcctgcggt ctcatggatc acgtgcgccg
      901 cgtcgatacc ggagagtgga ccgttcaggg cgatgtccat cagtaccact tcgggcttgt
      961 gttcggcaca aagctcgatg gcctcctccc cactggcgca tgaggccacc acggcgtgtc
     1021 cggcccgctt cagggcattc tcgatgtcca tgcggatgat ggattcatcc tccaccacca
     1081 gcaccctcgc actggtcgtt gtcacgggtt gtttttcctg taccatggtg catcggttta
     1141 cgcatcaacg atacgttaga acccattcgg acgatccgac ggatcgacga atgctggata
     1201 tcggaggatg gaaatgggtt gtaagccgat ctctgcggtc ggtggccgtc cttcaggttc
     1261 acgtgtgcaa aggtgcgagg gctccggttt tgaccttcgc acgcttgcgc gctccgccca
     1321 cggagaagac gggtgcttct ccgttgatcc agtagtagcg ggcggaatgg ttgtgccggt
     1381 acatgcggtg aatggatcat cgtccatcca gccaacgcag gatcgtctgg aggagcagct
     1441 cacgttgggc ggggtcggcc cggtcgctca tctcatggtg tttcacgttc gctaatggaa
     1501 ccaccacgat cccgtaccgg gcttgttc
//
LOCUS       DAOQOC010000872         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66718_length_1523_cov_4.219346, whole genome shotgun sequence.
ACCESSION   DAOQOC010000872 DAOQOC010000000
VERSION     DAOQOC010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66718_length_1523_cov_4.219346"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PL106_12645"
     CDS             complement(<1..1365)
                     /locus_tag="PL106_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNA33966.1"
                     /translation="MSTSAKHPKGLYVLFFTEMWERFGYYLMLGIFSLYMIDGWESGG
                     MGFSALKKSDIYGTYLGLVYLTPFIGGLLADRVLGFRRSILGGGLLMASGYLTLAIHN
                     ETTFYVGLLLIILGNGMFKPNISTLVGNLYNDDRYRANKDAGYNIFYMGINIGAFVCN
                     FVAAYMQIKYGWGYAFAAAGIGMLIGVAIFIGGNKHIKEADVMKPMQPGDMGTGRILL
                     STIAPMFLFGVLGYLIPGNLLGTDTNDAFIFGCIPVIAFFIYLYVKANEDDKRPIGAL
                     LAVFGCLVIFWAVFHQNGDALTVFAKEHTDREMSPAIAAIADPINMAETVTNQGADTD
                     ARSAGYFHSLAPEKVPAEGESMKLYSTQLYQSINPGWVILLTPLLVAFWAFMRSRGKE
                     PTTPTKIAIGLTITALSALVMVGAVMATNNLESKASSLWLIASYGVVTIGELCLSPMG
                     LSLVS"
BASE COUNT          298 a          444 c          484 g          297 t
ORIGIN      
        1 gctcaccagc gaaaggccca tcgggctcag gcacaactcg ccgatggtga ccacaccgta
       61 ggaggcgatc aaccacaagc tgctcgcctt gctctccagg ttgttggtgg ccattaccgc
      121 gcccaccatc accagggccg aaagtgcggt gatggtgagg ccgatggcga tcttggtggg
      181 tgtggtgggc tccttgcccc ggctgcgcat gaaggcccag aaggccacca gcaagggggt
      241 gagcaggatc acccagccgg gattgatgct ctggtacaac tgggtggagt acagcttcat
      301 gctctccccc tcggccggta ccttctccgg tgcgaggctg tggaaatacc ccgcgctccg
      361 ggcatcggtg tccgcgccct ggttcgtcac cgtttcggcc atgttgatcg ggtcggcgat
      421 ggcggcgatg gccggggaca tttctcggtc ggtatgttcc ttggcgaaca cggtgagggc
      481 gtcgccgttc tggtggaaca ccgcccagaa gatcaccagg cagccgaaga ccgccagcag
      541 cgcgccgatg ggccgcttgt catcctcgtt ggccttcacg tacaggtaga tgaagaaggc
      601 gatcaccggg atgcagccga atatgaaggc atcgttggtg tcggtgccca gcaggttacc
      661 gggaatgagg tagcccagaa cgccgaaaag gaacatgggg gcgatggtgc tcagcaggat
      721 gcggcccgta cccatgtcgc ccggttgcat gggcttcatc acatcggcct ccttgatgtg
      781 cttgttgccg ccgatgaaga tggccacacc gatgagcatg ccgatgcccg ccgctgcgaa
      841 ggcgtagccc cagccatact tgatctgcat gtaggctgcc acgaagttgc agacgaaggc
      901 accgatgttg atgcccatgt agaagatgtt gtagcccgca tccttgttcg cgcggtagcg
      961 gtcgtcgttg tacaggttgc ccacgagtgt gctgatgttg ggcttgaaca tgccattgcc
     1021 caggatgatg agcagcagcc ccacgtagaa ggtcgtttcg ttgtggatgg ccagcgtgag
     1081 gtagcccgaa gccatcagca ggccaccgcc gaggatgctc ctgcggaagc ccagcacccg
     1141 gtcggccagc aagcccccga tgaagggtgt gaggtagacc agtcccaggt aagtgccgta
     1201 gatatcgctc ttcttcaacg cgctgaaacc catgccaccg ctttcccagc cgtcgatcat
     1261 gtacagcgag aagatgccga gcatgaggta gtagccgaag cgctcccaca tctcggtgaa
     1321 gaaaaggacg tacaggccct tggggtgctt ggcgctggtg ctcatcggtc gatcggtccc
     1381 gcttggtacg tgagggtcat tcagttgcgg ctcacttggt gcatcaacgc accccgtgga
     1441 acagcttgtt gatcagcggg ctcaatacca tcaggaagag accgatgccc acggtcacgt
     1501 acccgatgtt gctgtacaca tcc
//
LOCUS       DAOQOC010000873         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66840_length_1522_cov_3.162236, whole genome shotgun sequence.
ACCESSION   DAOQOC010000873 DAOQOC010000000
VERSION     DAOQOC010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66840_length_1522_cov_3.162236"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PL106_12650"
     CDS             complement(<1..692)
                     /locus_tag="PL106_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33967.1"
                     /translation="MAKNAPIITRRRLLLGGVVAAAVAMAIALFGPGSSRGLYGASPF
                     GKPTDRDLDAIRRDTLRVLVVRNHLVYEHAPGVESGMEYELLKRLAHELNVPIKAVPV
                     ARPDSLLPLLQRGVGDVISANLGQRNPVARWTISSLPYRYVAPVFTTLRPDPYLGLNT
                     DLSVAPDTAWVSVWSTFAPRDLRFPGNDGKADLEKRTVFTDTSRFGDQAVINVALGHI
                     RAAIVSDGSAAF"
     gene            complement(765..>1522)
                     /locus_tag="PL106_12655"
     CDS             complement(765..>1522)
                     /locus_tag="PL106_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33968.1"
                     /translation="ATYSPGVSVSARVFLQGPYNSTDGLMADGLRTAGLIPNTEPFTA
                     LGFTHVNGGGGETLGAGVTSTSGADAVVDWVLLELRDAGSPAQVLATRSALVQRDGDI
                     TATDGVSPVVFGVGAGDYHVAVRHRNHLGAMTATAITLDEAPTPIDFTSVATGTFGTD
                     ARIAIGAVRALWTGNATGDATVKYTGGSNDRDPILLAVGSTTPNGSAAGYLRTDTNLD
                     GIVKYTGSANDRDIILTNTGSTTPNAVRAEQLP"
BASE COUNT          265 a          481 c          502 g          274 t
ORIGIN      
        1 aagaaggccg cactcccgtc cgatacgatg gctgcccgga tgtgcccgag cgccacgttg
       61 atgaccgcct ggtcgccgaa gcgcgaggtg tcggtgaaga ccgtgcgctt ctcgaggtcc
      121 gccttcccat cattgccggg gaagcggaga tcgcgtggtg cgaaggtgga ccacacactc
      181 acccaggccg tgtcgggagc tacggaaaga tcggtattca ggccgagata gggatcgggg
      241 cgtaacgtgg tgaataccgg ggccacatag cggtacggca atgaactgat cgtccagcgg
      301 gcgaccgggt tgcgttgtcc gaggttggcg gatatcacat cgcccacgcc ccgctgcaac
      361 aacggcagca agctgtccgg ccgggccaca ggaacagcct tgatgggtac gttcagttca
      421 tgggccagcc gtttgagcag ttcgtactcc atgcccgatt ccacaccggg cgcatgttcg
      481 tacaccagat gattccgcac cacgagtaca cgcaaggtgt cgcggcggat ggcatccagg
      541 tcgcggtcgg tcggtttgcc gaaaggcgag gctccataca ggccacgtga ggaccctggt
      601 ccgaacaagg cgatggccat cgccaccgcg gccgccacca cacctccgag aagcaggcgc
      661 ctacgcgtga tgatgggtgc gtttttcgcc atgcgtccgg ctcacggtgc gatatggttc
      721 aatactacga ccaacggtcc ttatcccggt cgcaccagct gttcctacgg tagttgttcc
      781 gcacgaacgg cgttcggggt ggtgcttccc gtattggtga ggatgatgtc gcggtcgttg
      841 gcgctgccgg tgtacttcac gatcccgtcc aggttggtgt cggtgcgcag gtaccccgca
      901 gcactgccgt tcggcgttgt gctgcccaca gccagaagga tcgggtcgcg atcgttgctg
      961 ccgccggtgt acttcaccgt cgcatcaccc gtggcgttcc ccgtccatag cgcgcgcacc
     1021 gcgccgatcg cgatccgtgc atccgtcccg aacgtgccgg tggccacgct ggtgaagtcg
     1081 atgggggtgg gtgcctcgtc aagcgtgatc gcggtggccg tcatggcgcc aaggtggttg
     1141 cggtgccgaa cggccacatg atagtccccc gcacccacgc cgaacaccac gggcgaaacc
     1201 ccatcggtgg cggtgatatc gccatcgcgc tgcaccagag cgctgcgcgt ggccagtacc
     1261 tgggctggtg aaccggcatc ccgtagttcc agcaggaccc aatcaacgac cgcatcggca
     1321 ccggaggtcg aggtgacgcc ggctccgagg gtctctcctc ctcccccgtt cacatgggtg
     1381 aagccgagtg cggtgaaggg ctcggtgttc gggatcagcc cggccgtgcg caggccgtcg
     1441 gccatcagac catcggtgct gttgtacggc ccttgcagga acacgcgtgc ggaaacactc
     1501 acaccgggcg agtaggtggc ac
//
LOCUS       DAOQOC010000874         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_66957_length_1521_cov_2.696453, whole genome shotgun sequence.
ACCESSION   DAOQOC010000874 DAOQOC010000000
VERSION     DAOQOC010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66957_length_1521_cov_2.696453"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PL106_12660"
     CDS             <1..220
                     /locus_tag="PL106_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33969.1"
                     /translation="AQLIARGKEAQWRPCAIKAAPLKDATNWMEQYRQFWEARFDRLD
                     EYLKELQAQEKPARATAASSSKKHGKRK"
     gene            204..677
                     /locus_tag="PL106_12665"
     CDS             204..677
                     /locus_tag="PL106_12665"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA33970.1"
                     /translation="MENASKIQEGAVGDREIIVTRIVHAPRERVWKAFATAEALAHWW
                     GPNGFTITTSAFEFREGGEWVFVMHGPDGRDYPNRIRFMRLVKPSRMEHEHGGDGDRV
                     VFDAVITLDDLGDTTRVTLHSTFRIASERDLVVKEYGAIEGGKQTLARLDEYLKQ"
     gene            719..1219
                     /locus_tag="PL106_12670"
     CDS             719..1219
                     /locus_tag="PL106_12670"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNA33971.1"
                     /translation="MTTPAIFNFIVKKEEKSITVERSFNAPLAAVWSAWTEADILCKW
                     WAPKPYACVIQSLDFRVGGSWRYYMQGPEGDCHYCSFDYTAVKPLTSFTGIDGFCDEH
                     GTPIDAMPRMNWENNFNAQDGSTVVRVQIRFNSEDDLEKITAMGFKEGFTQGLDQLDE
                     LLAIRK"
     gene            1300..>1521
                     /locus_tag="PL106_12675"
     CDS             1300..>1521
                     /locus_tag="PL106_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNA33972.1"
                     /translation="MSTATRITISALVNKPVAHVWKVWTEPEHITQWNAASDDWHCPK
                     ASNDLRTGGTFSSTMAARDDSFSFDFGGVF"
BASE COUNT          362 a          469 c          448 g          242 t
ORIGIN      
        1 tgcacagttg atcgcccggg gaaaggaggc gcaatggcgg ccgtgcgcca tcaaggctgc
       61 gccactgaag gacgctacca actggatgga gcagtaccgc cagttctggg aggcgcgctt
      121 cgatcggctg gatgagtacc tgaaggaact gcaggcacag gagaaaccgg cacgggccac
      181 agccgcgtct tcaagcaaga aacatggaaa acgcaagtaa gatccaggaa ggtgcggtgg
      241 gcgatcgcga gatcattgtc acccgcatag tacacgcccc gcgcgagcgg gtgtggaaag
      301 cctttgccac ggccgaagcc ctggcccatt ggtgggggcc gaacggcttc accatcacca
      361 cctcggcgtt cgagttccgc gagggcggtg agtgggtctt tgtgatgcac ggcccggacg
      421 gacgcgacta tccgaaccgg atccgcttca tgcgcctcgt gaagccctcg cgcatggagc
      481 acgagcacgg cggcgatggc gaccgcgtgg tcttcgatgc ggtgatcacc ctcgacgact
      541 tgggcgacac gacccgcgta acgctgcact ccaccttccg catcgcctca gaacgtgatc
      601 tggtggtgaa ggaatacggc gccatcgaag gcggcaagca aaccctcgca cgcctggacg
      661 aatacctcaa gcagtaacat ccggaaactt cccttcccaa caaacaaagc aagcaatcat
      721 gacaaccccg gccatcttca acttcatcgt caagaaggag gaaaagtcca tcacggtgga
      781 acgctccttc aacgcaccct tggcagcggt atggtccgca tggacagaag ccgatatcct
      841 gtgcaagtgg tgggcaccga agccgtacgc atgcgtgatc cagtcgcttg acttccgtgt
      901 gggcggaagc tggcgctact acatgcaggg cccggaaggc gattgccact attgcagctt
      961 cgactacacg gccgtgaaac ccttgacctc cttcaccggg atcgatggct tctgcgatga
     1021 gcacggtacg cccatcgacg cgatgcctcg gatgaactgg gagaacaact tcaacgcaca
     1081 agatggcagc acggtggtgc gcgtgcagat ccgcttcaac agcgaggatg atctggagaa
     1141 gatcacggcc atgggcttca aggaaggttt cacgcagggg ctggaccaac tggatgagct
     1201 gctcgcgatc aggaaataag ggcgcgagat cacgcgctcc aaaaactaaa aacaagtaag
     1261 ggcgggaaat ttcccgcccc gacaaacaac cccgacccca tgtcaaccgc aacccgcatc
     1321 accatctccg cccttgtgaa caaacccgtg gcccacgtct ggaaagtctg gacggagccc
     1381 gaacacatca cgcaatggaa cgccgccagt gacgactggc actgccccaa ggccagcaac
     1441 gacctgcgca caggcggcac cttcagcagc accatggccg cacgcgacga cagctttagc
     1501 ttcgacttcg gcggcgtgtt c
//
LOCUS       DAOQOC010000875         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67092_length_1519_cov_4.002049, whole genome shotgun sequence.
ACCESSION   DAOQOC010000875 DAOQOC010000000
VERSION     DAOQOC010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67092_length_1519_cov_4.002049"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1078
                     /locus_tag="PL106_12680"
     CDS             437..1078
                     /locus_tag="PL106_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNA33973.1"
                     /translation="MAAPKPGPSRIFENRFLEALTRTHIAIPLSLFWTAGAITLWYSI
                     ARLDVSALTAVLLFLTGALFFTLIEYLMHRFLYHIPAATVGRRKFQYTIHGVHHDHPR
                     DKQRLAMPPLVSVVLAVLFIALFRLVMGEYGYAFGGGFLFGYSTYLAAHYAIHMFKPP
                     KNFLSILWKHHNLHHYVGDDGAFGVSSPFWDHVFGTMPPDPKRRAAERTPGLL"
     gene            1159..>1519
                     /locus_tag="PL106_12685"
     CDS             1159..>1519
                     /locus_tag="PL106_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33974.1"
                     /translation="MAWQRSAIFTGHKAGVYALCPGTEPWSFLSAGGDGIVSRWDRRT
                     NDHGEQVAGSPKALWSMHFDRGRNMLFLGDSEGGLRTAHPGQGEARHLVAHARGIFSM
                     VPLGNDRIVCAGGDGSIS"
BASE COUNT          269 a          506 c          454 g          290 t
ORIGIN      
        1 caacggccca acggcccaac ggcccaacgg cccaacggcc caacggccca acggcgccct
       61 ttgcccgccg aatggcggac caacgcgcaa gccaagcaag aaggctagtt gaagttgcgc
      121 gttcatgttg gaacgaatgt aacggaattt attgggtgtg atagcagaag tggaaaagtc
      181 gtggcgcagg gtccgcgaag agccgaaggt tccggttggc gagaccctgc gggagggagc
      241 gtcccgccat ggaccctgtt gacgggtttt cagaacaagc tttttgccgg ttttccgata
      301 ccggacccgt cgctcgtcag ggcgacgtcc ggatgatgcc gatccaccgc ggtcgcagcc
      361 tctcgtgcca tgggacgtgg ccgtgatcat gttctttcct gatgaccgtg taatttcgcc
      421 cctgtaaccc gttgtgatgg ccgctccgaa accaggaccc agcagaattt tcgagaaccg
      481 cttcctcgaa gcgctgacca ggacgcatat cgcgatcccc ctttcccttt tctggaccgc
      541 cggggccatc acgctctggt attccatcgc ccgcttggac gtgtccgcat tgaccgccgt
      601 gctgctcttc ctgacaggcg cgctattctt cacgctgatc gagtacctga tgcaccgctt
      661 cctgtaccat attccagcag cgacagtggg ccgcagaaag ttccagtaca ccatccacgg
      721 ggttcaccac gaccacccgc gcgacaagca gcgcctggcc atgcctccgc tggtcagtgt
      781 ggtgctcgcc gtgctcttca tcgccctgtt ccgtctggtg atgggggagt acggctacgc
      841 tttcggcggt gggttccttt tcggctacag cacctacctg gcggcccact acgccatcca
      901 catgttcaag ccgcccaaga atttcctgtc catcctgtgg aagcaccaca acctgcacca
      961 ttacgtgggg gatgacggtg ccttcggtgt gagcagcccg ttctgggacc atgtcttcgg
     1021 caccatgccg cccgacccca agcgcagggc cgccgagcgg acaccgggac tgttgtaacg
     1081 gccctcgctc ccaccagcgc gcatgggccg cgcgtgaagc gatcgttctc cccgctcgca
     1141 gctttccacc cgcacaccat ggcctggcaa cgctccgcga tcttcaccgg acacaaggca
     1201 ggggtgtacg cgctctgccc cggtacggaa ccgtggagct tcctgagcgc gggtggcgac
     1261 ggcatcgttt cgcgctggga ccggcgcaca aacgatcatg gcgaacaggt agctggatcg
     1321 ccaaaggccc tctggtccat gcatttcgat cgcgggcgga acatgctttt cctgggcgac
     1381 agcgaaggcg gcctgcgcac ggcccatccc ggtcagggcg aggcgcgaca cctggtggcc
     1441 cacgcgcgcg gcatcttcag catggtgccc ctgggcaacg accgcatcgt gtgcgcgggt
     1501 ggcgatggct ccatcagcg
//
LOCUS       DAOQOC010000876         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67174_length_1518_cov_3.663705, whole genome shotgun sequence.
ACCESSION   DAOQOC010000876 DAOQOC010000000
VERSION     DAOQOC010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67174_length_1518_cov_3.663705"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PL106_12690"
     CDS             <1..987
                     /locus_tag="PL106_12690"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33975.1"
                     /translation="HGTLPTTANEADHGPQAIGANVVFYNTENLFDAEDDPHTNDNDF
                     LPEGELRWTGERYAHKLEQIAKAIGMNGPHAPCLIGLAEVENRRVLEDLSRTAGLAQG
                     AYAIVHKDSDDPRGIDVALLVSEHFGRVKAQEFLPVPLRSGVTRPILHAVVETSGNQR
                     IHVFVNHWPSRRAGEGETRGKRMAAAQVLRSALDEVFDSGSEPYVIVMGDFNDGPSDA
                     SISRVLEGGGKSLDAGKDLVNLVALDQEDPLGSISHGGTWEYFDQIMVSRALLAARGT
                     GHVRAISAASVKDPRLIFDHPRYGAQPDRTFSGTRYHRDGYSDHLPVVLRME"
     gene            complement(1073..>1518)
                     /locus_tag="PL106_12695"
     CDS             complement(1073..>1518)
                     /locus_tag="PL106_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNA33976.1"
                     /translation="EPVYNGRNAAAAAVAAHECGHAVQHATAYRWLTMRSKLVPAVQL
                     SSTVMPWVIMGGIFMVNTFPQLLLAGIVLFAMTTLFSFITLPVEYDASNRALAWIKNN
                     GIVTTSEYAMSADALKWAARTYVISAIGSLATLLYYLSIYMGRRN"
BASE COUNT          300 a          463 c          496 g          259 t
ORIGIN      
        1 cacggcacgc ttccgaccac ggcgaacgag gcggaccatg gaccacaggc gatcggtgcc
       61 aacgttgtgt tctacaacac ggagaacctt tttgatgcgg aagatgaccc gcataccaac
      121 gacaacgatt ttctaccgga aggcgagttg cgttggaccg gggaacgcta cgcgcacaag
      181 ctggaacaga tcgccaaggc catcgggatg aatggaccgc acgcgccctg cctcatcggc
      241 ctggccgaag tggagaaccg tcgagtcctg gaggacttgt cgcgaacggc tgggctggcc
      301 cagggggcat atgcgatcgt ccacaaggat tcggacgatc cgcgcggcat cgatgtggcc
      361 ctgctggtct ccgaacactt cgggcgggtg aaggcccagg aatttctccc ggtcccactg
      421 aggtccggtg tgacccgacc gatcctgcac gccgtggtgg aaacaagcgg gaaccagcgc
      481 atccacgtct tcgtgaacca ctggcccagc cgcagagcag gggaggggga aacccgtggg
      541 aaacgcatgg cggcggccca agtgctccgc agcgcactgg atgaggtgtt cgacagcggg
      601 tccgaaccgt acgtgatcgt catgggcgat ttcaacgatg ggccttccga tgcgagtatc
      661 agcagggtgc tggagggtgg cgggaagtcg ctggatgccg ggaaagacct cgtgaacctg
      721 gtcgctttgg accaggagga tcccttgggc agcatcagcc atggtggcac gtgggagtac
      781 ttcgaccaga tcatggtgag ccgtgccttg ttggccgcac gcggcaccgg ccatgtccgc
      841 gctatctccg cagcttcggt gaaggatccc cggctgatct tcgaccaccc caggtatggt
      901 gcccagcccg atcgcacctt cagcggcacc aggtaccacc gcgacggcta cagcgatcac
      961 ctgccggtgg tgctgcgaat ggaatgatcc gccagcgacg ggatccctct acggtctgaa
     1021 gaggaaagcc cggtgagcgc cgggctctcc gaggtgctga tcggctgcga gatcagttgc
     1081 ggcgacccat gtagatcgaa aggtagtaca gcaacgtggc cagcgagccg atcgccgaga
     1141 tcacgtacgt gcgcgcggcc cacttcaacg catcggcgct catggcatac tcgctcgtgg
     1201 tcacaatgcc gttgttcttg atccaggcca atgcccggtt gctggcatcg tactccaccg
     1261 gcaaggtgat gaagctgaac agcgtggtca tggcgaaaag aactatcccg gccagcagca
     1321 gttggggaaa ggtgttgacc atgaagatac cgcccatgat cacccacggc atcaccgtgc
     1381 tgctcaactg caccgccgga accagcttgc tccgcatggt gagccagcgg taggccgtgg
     1441 cgtgctgcac cgcgtgcccg cactcgtgtg ctgccacggc ggcggcggcg gcgttgcgcc
     1501 cgttgtacac cggctcgc
//
LOCUS       DAOQOC010000877         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67222_length_1518_cov_2.283664, whole genome shotgun sequence.
ACCESSION   DAOQOC010000877 DAOQOC010000000
VERSION     DAOQOC010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67222_length_1518_cov_2.283664"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..>1518)
                     /locus_tag="PL106_12700"
     CDS             complement(103..>1518)
                     /locus_tag="PL106_12700"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33977.1"
                     /translation="SPHLQRIEERLTVDGVPCDVVSFTSGTAVATAVEVPPYRPFNSL
                     SDPTGTALVHAVHTVVLNAQLDTMSGCPALDTGEGIQGFLFLPFPGDTNDLAFFHNHA
                     TNSGCELSFGRIDLAQDNGLGAFVGGMTLLNNWTASKLTAVAHANGQDYWVLTQILGT
                     DEIAAYLLSATGVDSVPVISHTGVPMIAQFDGTEAVQDRGAMVASYTGDRLAMAGLVS
                     SNVEPYTLDHARIEVLHFDNATGSATHAASIPPPTGNTINGIEFSPDGSKLYFTSKSA
                     LELPITSTLHQYDLSVQDEAAIIASHTVIFTEEVGSFDVQDPFGQLALAPDRRIWFIR
                     WPGSPWLGVIESPNEAGAACTPVMDQLLLPHNAGAGLPSQCKRYHDSELTHTGIPASE
                     EAGTLMTWPNPAQTLLNINAPEPGCTSQLYDATGRLVLSTMHGPRRTATVDVAGLPAG
                     PYLVRCTDALGNGWTSTMLKE"
BASE COUNT          279 a          448 c          491 g          300 t
ORIGIN      
        1 ctcaccctct caacctttct caatgatcct cctccatcat cgccgggcgc tgcgtcagct
       61 cgtggtagca aagtacatcg tcggcgaagc aaggttcgac ctttactcct tcaacatcgt
      121 gctcgtccag ccgttgccca gcgcatcggt acaacgaacc aggtagggcc ctgcgggtag
      181 tcccgccacg tcaacggtcg ccgtacggcg cggcccgtgc atggtggaaa gcaccagacg
      241 gccggtggca tcatacagtt gtgatgtgca gcccggttcc ggcgcattga tgttcagcaa
      301 ggtctgtgcg gggttgggcc aggtcatcag cgtgcccgcc tcttccgatg ccggaatacc
      361 ggtatgggtc agctcgctgt cgtggtagcg tttgcattgg ctgggcaatc cggctccggc
      421 gttgtgcgga agaagcagct gatccatgac cggtgtgcag gccgcccccg cttcgttggg
      481 cgactcgatc acgccgagcc atggtgaacc cggccaacgg atgaaccaga tccgccgatc
      541 cggggccaag gccagttgcc cgaatggatc ctggacgtca aagctgccga cctcctcggt
      601 gaagatcacg gtgtgcgatg cgatgatggc ggcctcgtcc tgaacggaca ggtcgtattg
      661 atgcagggtg ctggtgatcg gtaattccag ggccgacttg ctcgtgaagt agagctttga
      721 accgtcggga gagaactcga tgccgttgat ggtgttgcca gttggtggag ggatggaggc
      781 cgcatgggtg gcgctgcccg ttgcgttgtc gaaatggagg acctctatgc gggcatgatc
      841 gagggtatac ggctccacgt tgctggaaac caggcccgcc atggccagcc ggtcgcccgt
      901 gtacgaggcc accatcgcgc cacgatcctg cacagcttcg gtgccatcga actgtgcgat
      961 catcggtaca ccggtatgac tgatcaccgg cacactgtcc acgccagtgg cgctcaacag
     1021 ataggcggcg atctcatcgg tgcccaggat ctgcgtgagc acccagtaat cctgcccatt
     1081 ggcgtgcgcc acggcggtga gtttgctggc ggtccagttg ttcagcagcg tcatgccgcc
     1141 gacgaacgcg ccaagcccgt tgtcctgcgc caggtcgatg cgcccgaacg agagttcgca
     1201 tccggaattg gtcgcgtggt tatggaagaa ggccaggtcg ttcgtatcac ctggaaacgg
     1261 taaaaaaaga aagccttgga tgccttcgcc ggtatcgagg gccgggcatc cgctcatggt
     1321 atccagctgg gcattcagca ccacggtgtg cacggcatgg acaagcgcgg tcccggtggg
     1381 atcgctcaaa ctgttgaagg gccggtaggg gggtacctca accgctgtgg ccactgcggt
     1441 gccgctggtg aacgagacca catcgcaggg gacgccgtcg accgtcagtc gttcctcgat
     1501 ccgttgcaga tgcggcga
//
LOCUS       DAOQOC010000878         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67276_length_1517_cov_3.451436, whole genome shotgun sequence.
ACCESSION   DAOQOC010000878 DAOQOC010000000
VERSION     DAOQOC010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67276_length_1517_cov_3.451436"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PL106_12705"
     CDS             complement(<1..1489)
                     /locus_tag="PL106_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33978.1"
                     /translation="MRDKRPFIIAQGTVFTIAPDKITSESVGITLKLDNDSIHHPSVS
                     LRFRKDKKQLTCIKNNEGLSMAPFWDSYHQLDMQFEELRWKQGDPLVEIGNLQGSAQT
                     KTVYESYNYFKELRYKAMLGIDAVHPLSRLSDYQKVAGDVFQAGDFAKYTKLQKPQVI
                     PMMFDMAVKRYIDYDPESETVRILPRLKEHTQASARRMDYDALQFNSDVADGVNGTIN
                     LLNNDLVLKGVARIILSDSQDVRLFPSERTVTVKKDRDFSFGGKITAGRLNFFGKEYY
                     FHYAPFTIDLLNVDSVSFYADSFEPEANGDRPLVRVKNVLEKVTGTLEIDAPSNKSGI
                     QQVMREGRKEPRYPEYPKFNSTKESYVFYDRGDIQKGAYKRDKFYYKSDPFTIDSLDN
                     FTNAGLTFTGTLMSGGIFPDIREPITLQHDYALGFVRPTGQGGLPLYGKKAKFANTLK
                     LDHAGLHGEGDFTYLTTIATSKDLYFTPDTTFGVADTLYNGAATSP"
BASE COUNT          242 a          426 c          493 g          356 t
ORIGIN      
        1 tggggctggt ggccgcgccg ttgtacaggg tatccgctac gccgaacgtg gtatcaggtg
       61 tgaaatagag gtctttgctc gtagcgatgg tggtgaggta ggtgaaatcc ccctccccgt
      121 gcaggccagc gtggtcgagc ttgagggtgt tggcgaactt ggccttcttg ccgtacaggg
      181 gcaggccacc ctgaccggtc gggcgcacga agcccagggc gtaatcgtgt tggagggtga
      241 tgggctcccg gatgtcgggg aagatcccgc cgctcatgag cgtgccggtg aaggtgagcc
      301 ccgcattggt gaagttgtcc agcgagtcga tggtgaacgg gtcgctcttg tagtagaatt
      361 tgtcccgctt gtaggcgcct ttctggatgt cgccccggtc gtagaacacg taactctcct
      421 tcgtgctgtt gaacttgggg tactccgggt agcgcggttc cttgcggcct tcgcgcatca
      481 cctgctgtat gccgctcttg ttgctcgggg catcgatctc cagggtgccg gtcaccttct
      541 ccagcacgtt cttcacacgc accagggggc ggtccccgtt ggcctcgggc tcgaagctgt
      601 cggcatagaa actcacgctg tccacgttga gcaggtcgat ggtgaagggg gcgtaatgga
      661 agtagtattc cttgccgaag aagttgagcc gccccgccgt gatcttgccg ccgaagctga
      721 agtcgcggtc cttcttcacc gttacggtgc gctcactggg gaagagccgc acgtcctgcg
      781 aatcgctcag tatgatgcgt gccacgccct tgagcacgag gtcgttgttc agcaggttga
      841 tcgtcccgtt cacgccgtcg gccacatcgc tgttgaattg cagggcgtcg tagtccatcc
      901 gtcgggcact ggcctgggtg tgctccttca agcgcggcag gatccgtacg gtctcggatt
      961 ccggatcgta atcgatgtac cgcttcaccg ccatgtcgaa catcatgggg atcacctggg
     1021 gcttctgcag cttggtgtac ttggcgaagt cgcccgcttg gaacacgtca ccggccacct
     1081 tctggtagtc gctcaaccgg ctgagcggat ggaccgcatc gatgccgagc atggccttgt
     1141 accggagctc cttgaagtag ttgtagctct cgtagaccgt cttggtctgg gcactcccct
     1201 gcagattgcc gatctccacc agcggatcgc cctgcttcca gcgcaattcc tcgaactgca
     1261 tgtccagctg gtggtagctg tcccagaaag gtgccatgct gaggccctcg ttgttcttga
     1321 tgcaggtgag ttgcttcttg tccttgcgga aacgcaggct gacgctgggg tggtggatgg
     1381 aatcgttatc gagtttcaag gtgatgccca cgctctcgct ggtgatcttg tccggagcga
     1441 tggtgaacac cgtgccctgc gcgatgatga atggccgctt gtcccgcatg aaggtgaggt
     1501 aggcgggggc ttcgcgc
//
LOCUS       DAOQOC010000879         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67356_length_1516_cov_4.922656, whole genome shotgun sequence.
ACCESSION   DAOQOC010000879 DAOQOC010000000
VERSION     DAOQOC010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67356_length_1516_cov_4.922656"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1214
                     /locus_tag="PL106_12710"
     CDS             228..1214
                     /locus_tag="PL106_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNA33979.1"
                     /translation="MRHHDGPGVDHAEVIRSALFGVAVGDALGVPVEFTNRARLSKDP
                     VTDMIGHGTYDLPAGTWSDDSSLTFCLAEALTQEFDLDTIGRNFVKWYHEGYWTPYGN
                     VFDIGITTREAINRIARGAKAGSAGGTDENANGNGSLMRILPLVFHLANRPVDERFRI
                     TQQVSSITHGHIRSAIACFYYLEFAKRIMEGEDKFGIHRDLGTEVLDHLTARSTDPME
                     IAVFDRLLKGDIHHLDQDRIESGGYVMHTLEASIWCLLTTDNFKEAVLKAVNLGRDTD
                     TTGAVTGGLAGLLYGMKNIPENWLRKLARYDDIQDLVARLAVKLPGHQRTGR"
     gene            1294..>1516
                     /locus_tag="PL106_12715"
     CDS             1294..>1516
                     /locus_tag="PL106_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNA33980.1"
                     /translation="MPLARYHTRGLLEAGCDEAGRGCLAGPVVAAAVILPEKARLPGL
                     NDSKQLTEAQREKLRPLIEKQAIAWAVAHA"
BASE COUNT          326 a          412 c          491 g          287 t
ORIGIN      
        1 caaaagcttg tgcgatctgg agagaaagga cggctacttc atttacagaa tgggcgacct
       61 tcgtttccgg atatctcctt ccgctcgagg tgtctgggac cgggcggctc tttggttgtc
      121 gatgcgtaga ttcattgtca gagtgaggga caagaagaac cctccggaat ggatcgatgg
      181 gcctgcaggg agcgcacgcg ggtacatcga tccaatgatg ctgggcaatg aggcaccatg
      241 atggaccggg cgttgatcac gccgaagtga tcaggtccgc acttttcgga gttgcggtgg
      301 gcgatgcgct gggtgttccg gtcgaattca cgaacagggc aaggctgagc aaggaccctg
      361 taacggatat gatcggtcac gggacgtacg acctgcccgc agggacctgg tcggacgaca
      421 gttcgctgac cttctgtctc gcggaagcat tgacgcagga attcgacctc gacacgatcg
      481 gacggaattt cgtgaaatgg tatcatgagg gctattggac accgtacgga aatgtcttcg
      541 atatcggtat cacgacacga gaggccatca accggatcgc aagaggggcg aaagcgggat
      601 cggcaggcgg cacggacgag aacgcgaatg ggaacggttc gttgatgcgg atcctgccgt
      661 tggtgttcca tcttgccaat aggccggtcg acgaacgctt ccggatcacc caacaggtct
      721 cgtcgatcac ccacgggcac atccggtccg cgatcgcgtg cttctactac ctcgaattcg
      781 cgaagcggat catggagggc gaggacaagt tcgggatcca cagggatctg ggaaccgagg
      841 tgcttgacca cttgacggca cgctccacgg atcccatgga gatcgccgtg ttcgaccggt
      901 tgttgaaagg cgacatccac cacctcgacc aggaccggat cgaaagcggc ggctatgtga
      961 tgcatacgct ggaggccagt atctggtgtt tgctgaccac cgacaatttc aaggaagccg
     1021 tcctgaaggc ggtcaatctg ggcagggaca cggacacgac aggtgcggtc accggcggcc
     1081 ttgccggcct gctgtacggc atgaagaaca ttcccgaaaa ctggttacga aaattggccc
     1141 gatatgacga catccaggac ctggtggcac gactggcggt caaacttccc ggccaccaac
     1201 gaaccggccg gtaactgcgc gggtatgtgg tcatgaggag gtcggatacg tgctcgagcg
     1261 gtggagctct ggccacctct atctttccgc accatgccac tcgcccgtta ccacacccga
     1321 ggtctgctgg aagcgggatg cgatgaggcg ggtcgtggat gcctggcggg cccggtggtg
     1381 gccgctgcgg tgatcctgcc ggagaaggcg cgtctgccgg gcctgaacga ctccaagcag
     1441 ctcaccgaag cacagcgcga gaaacttcgc ccgttgatcg agaagcaagc gatcgcttgg
     1501 gcggtggcac atgcct
//
LOCUS       DAOQOC010000880         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67387_length_1516_cov_3.176591, whole genome shotgun sequence.
ACCESSION   DAOQOC010000880 DAOQOC010000000
VERSION     DAOQOC010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67387_length_1516_cov_3.176591"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PL106_12720"
     CDS             <1..423
                     /locus_tag="PL106_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33981.1"
                     /translation="DRKGLVKWDHPRKDGRYRKGKVKCPFKNMKSETVKADVSLYLTG
                     DANGLIEWRMGSGYSREWNGIGLASSFWSEVDSLRYEELGSCHQDGPGVNEQTTYKLN
                     LPGKQPLWVEESSSCGTMGCDFSLTFSLVPFERRLPCD"
     gene            529..963
                     /locus_tag="PL106_12725"
     CDS             529..963
                     /locus_tag="PL106_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33982.1"
                     /translation="MKTLTIAFCIAQVLSNTLTAQVFHPMTDEAIAARIAEEASAELG
                     LANESLYRLECRIASRNEGMAGTGAALVMYAPARAHLVVAFDLVSVKTGSVIRTGEAH
                     VNGRNSMDEAIEEAVERISMRNDLMPTGPGTPGPVNVIARTH"
     gene            1048..1182
                     /locus_tag="PL106_12730"
     CDS             1048..1182
                     /locus_tag="PL106_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33983.1"
                     /translation="MEQSPQSHGSRLLGWVAAIAFAATVGCYFLVFFTVQVGDAEPET
                     "
BASE COUNT          333 a          445 c          441 g          297 t
ORIGIN      
        1 gaccgcaaag gacttgtgaa atgggatcat ccacgcaagg atggccgtta tcgcaagggc
       61 aaggtaaagt gcccattcaa gaacatgaag tccgaaacgg tgaaggccga tgtgtcgctc
      121 tacttgaccg gagatgcgaa cggactgatc gaatggcgca tgggctcagg gtacagccgc
      181 gaatggaacg ggatcggact tgcctcttcc ttttggtcgg aggtggacag ccttcgctat
      241 gaagaattag gatcttgcca ccaggatggt ccgggggtga acgaacagac cacctataag
      301 ctgaacctgc ccggaaaaca accgctatgg gtggaggaga gcagttcgtg cggcaccatg
      361 ggctgtgact tcagtctcac gttcagcctg gtcccgttcg agcgtaggtt gccgtgtgat
      421 tgacctcctt tccaccgcat tccacccctt ccccgccgct ttcctccgca cactggcatt
      481 cgcatatggg accctgttgt ttcgcctcat caacgaaaca caacagccat gaaaaccctg
      541 accatcgcct tctgcatcgc gcaagtactg tccaacacat tgaccgctca agtcttccat
      601 cccatgaccg atgaggccat cgccgcacgg atcgcagagg aggcaagcgc cgaactcggt
      661 cttgccaacg aaagcttgta ccgactggag tgccgcatcg catcgcgtaa cgaaggcatg
      721 gccggaacgg gcgctgcgct ggtgatgtac gctccagcca gggcgcacct ggtggtggcc
      781 tttgacctgg tttcggtgaa gaccgggtcg gttatccgca ccggcgaggc gcacgtgaac
      841 ggcaggaaca gcatggatga ggcgatcgag gaagcggtgg agcggatctc catgcggaac
      901 gacctgatgc ctaccggtcc tggtacaccc ggaccggtga atgtgatcgc acggacccac
      961 tgatcttgca ctggtaacac ttcattgccg aagggtaacc attccagtgt gaccccaacc
     1021 gtggaacgtt gaaccgtccc tcgaacaatg gaacagtcgc cacagagcca tggatcacgg
     1081 ctgctcggat gggtggccgc catcgctttc gcggccacgg tcgggtgcta cttcctggtg
     1141 ttcttcacgg tgcaggtggg cgatgccgag ccggagacct aaaaaggtat cgacctcgtg
     1201 ctccgtacca aagcccagca ctatagccgc tccttcctgc gtgaacacgg atgaaatgtg
     1261 cctgacaacg aaacgaattc tgaaccacct cccgacaaca attcggcgga acggtcaggt
     1321 cggcatgtgc acgtgaagca acaggaacgg gcggtatccg cggctcagcg tgttccgttc
     1381 cgcagcggta gcgtgtggca tcggcgagct gatccatgct gtgctactga taaccggtgg
     1441 aagggacccg ccctgaactc ccctcaccag caccgcattc gtggttgccg atactttcac
     1501 ggtgacacct gtattc
//
LOCUS       DAOQOC010000881         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67568_length_1514_cov_3.056888, whole genome shotgun sequence.
ACCESSION   DAOQOC010000881 DAOQOC010000000
VERSION     DAOQOC010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67568_length_1514_cov_3.056888"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1164)
                     /locus_tag="PL106_12735"
     CDS             complement(160..1164)
                     /locus_tag="PL106_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA33984.1"
                     /translation="MRDEPHIRDVLHGSARTLVLLGCGLLAVLAHGQYLGGQGRGTVQ
                     SGCEPSDCLDVPGGSAMPGTSCDDGDACTIADVYMASCQCAGTPSPDSDGDLICDILD
                     ICPNDPFNDQDADGICGDVDNCPTVPGLIGDTCNDGNCFTLEDVITPDCVCTGTGTGE
                     EGSILGNTILADGLSYWFSTTDVVGAQYTWSILPNAVGWQLNAQGAVASVVAPSSNAE
                     VELCVSATLNDTICVVQACTPLFVMDVGSMPMPTLEHGITIRPNPSDGRFELRRSTQM
                     SGPVLFEVLDAMGRSVIPTTVMSSTGTLIDLGPASPGVYLVKIRDSEGAHVLRAAKSR
                     "
     gene            complement(1193..>1514)
                     /locus_tag="PL106_12740"
     CDS             complement(1193..>1514)
                     /locus_tag="PL106_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33985.1"
                     /translation="AQAGAGYYGVLDLGGNVGELAVTIGFPNGRAFDGSLGDGELDSL
                     GLADEPTWPVTGYFSGVGVLGGAWILDNTYMRLSDRHTASHFTYTSRSGVNGGRGVRD
                     APVE"
BASE COUNT          295 a          509 c          429 g          281 t
ORIGIN      
        1 ggcaggtatc tgaacgcgtc cgatctgaat ttgaacggaa cgcccaagga ggatccactg
       61 cttccgtccg gcgggcccaa actgagcgcg atctccttcg ccgtgggtgt gatgttcagg
      121 ccggatccgg tcgccagaaa gcggttctga ccagcgaagc taccggctct ttgctgcccg
      181 gaggacatgg gcaccttcgc tgtcccggat cttcacgagg tatacacccg gtgatgcagg
      241 acccagatcg atcagggtac ccgtgctgct catgaccgtt gtggggatca ccgatcgacc
      301 catggcgtcc aacacctcga aaagaaccgg tccactcatt tgcgtggagc gtcgtagttc
      361 gaatcgtcca tcgctcgggt tcggacgaat ggtgatgccg tgctccaacg tcggcatggg
      421 catggatccc acgtccatca cgaacagggg tgtgcatgct tgtaccacac agatcgtatc
      481 attcagcgtg gccgaaacac acagttccac ttcagcattg ctggagggcg cgaccacgga
      541 cgcgacggca ccttgtgcgt tcagctgcca gcccacggca ttgggcagga tggaccacgt
      601 gtactgcgca ccgaccacat cggtggtgct gaaccagtaa ctcagcccgt cggccaggat
      661 cgtgttcccc aggatgctgc cctcctcgcc cgtgcccgtt ccggtacata cgcagtccgg
      721 cgtgatcaca tcctcaaggg tgaagcagtt gccatcgttg caggtgtccc cgatcaggcc
      781 cggtaccgtt ggacagttgt ccacatcgcc gcagatgcca tcggcatcct gatcgttgaa
      841 ggggtcgttg gggcagatat ccaggatgtc gcagatcaga tccccatcgc tgtccgggct
      901 gggtgttccg gcacactggc aactcgccat gtacacatcc gcgatcgtac acgcgtcccc
      961 atcatcgcac gaagtccccg gcatggccga tccgcccggc acatcaaggc aatccgaagg
     1021 ctcgcaaccg ctctggaccg tgcccctgcc ctggccaccc aggtactgcc catgtgccag
     1081 gaccgccagc aggccgcacc ccagcagcac aagggtccgg gccgatccgt ggagaacatc
     1141 gcgtatgtgg ggttcgtccc tcatggcttc gatcgatggc gtcctatccc actcattcca
     1201 ccggtgcatc ccgcacaccc cgaccaccat tcactcccga tcgggaggta taggtgaagt
     1261 gggatgcggt atggcggtcg gaaaggcgca tatacgtgtt atcgagtatc caagccccac
     1321 caagcacccc gaccccgctg aagtatcccg tgaccggcca tgtgggctca tccgcgaggc
     1381 ccagactgtc cagttccccg tcccccaacg acccatcgaa cgcacggcca ttggggaagc
     1441 ctatggtcac agccagttcg cccacgttgc cgcccaggtc caggaccccg taataacccg
     1501 cacccgcctg cgca
//
LOCUS       DAOQOC010000882         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67632_length_1513_cov_3.839506, whole genome shotgun sequence.
ACCESSION   DAOQOC010000882 DAOQOC010000000
VERSION     DAOQOC010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67632_length_1513_cov_3.839506"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PL106_12745"
     CDS             complement(<1..1171)
                     /locus_tag="PL106_12745"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNA33986.1"
                     /translation="MALYNLFIFASIRDRSYLIYVMYIAVICLAQLAVLGIGQSYLWP
                     DNVWLASRSSIVLVLGAMTLGMEFARRFIGTSQVVPRLHRLVPIFYLAVFANVVLYFA
                     GDPWTGYIMAQALGGWSAIYLLIMVITAMRKNSRQAGYFLLAWGFFLFGVVVFVMRDA
                     GILPYTSLTTYAMPIGSAVEGVLLSFALADRINIMRKEKELSQAEALSAAQENARITR
                     DQNILLEMKVSERTEQLQHSLDQLKRAQSKLVEAERMSSLGQLTAGIAHEINNPVNFI
                     RSNIAPLKRDMDDIFQVLVSYRSHDVPPHVAELERSLGLDETIDEVRHILDSIEEGAD
                     RTSEIVRGLRSFSRLDEDTLKYASVDQGIRGTLNLLGPKLKKGINVELDLHASQTV"
BASE COUNT          323 a          437 c          436 g          317 t
ORIGIN      
        1 cgacggtctg cgacgcgtgc aggtccagtt ccacgttgat ccccttcttc agtttgggcc
       61 ctaacaggtt cagcgtaccg cgaatgccct gatccacgct cgcgtacttg agcgtatcct
      121 catccaagcg gctgaaagac cgcaatccgc gaacgatctc gctggtacga tcggcccctt
      181 cttcgatgct gtccagtatg tgccgcactt catcgatggt ctcatccagg ccgagggaac
      241 gctcaagctc cgccacatgg ggtggtacat cgtgcgatcg gtaggagacc agtacttgga
      301 agatgtcgtc catatcgcgc ttgagcgggg cgatgttgct gcggatgaag ttgaccgggt
      361 tgttgatctc gtgcgcgatg cccgcggtga gttggcccag ggacgacatg cgctcggcct
      421 ccaccagctt gctctgggcg cgtttgagct ggtccaggct gtgctgcagc tgctcggtgc
      481 gttcgctcac cttcatctcc agcaagatgt tctgatcccg cgtgatgcgc gcattctctt
      541 gtgcggcgga gagcgcctcg gcttgggaga gttccttctc cttgcgcatg atgttgatcc
      601 ggtcggccag cgcgaaggag aggagcacgc cttccaccgc cgagccgatg ggcatggcgt
      661 aggtggtaag gctcgtgtac gggagaattc cggcatcgcg cataacgaag accaccacac
      721 cgaaaagaaa gaagccccag gccagcagga agtatccggc ttggcgagag ttcttgcgca
      781 tggcggttat taccatgatg agcagataga tcgccgacca gccaccgagt gcttgcgcca
      841 tgatatatcc ggtccacgga tcaccggcga agtacaacac aacgttcgcg aacacggcaa
      901 gataaaatat cggcacaagc ctatgaagcc ttggtacgac ttgcgaagta ccgatgaatc
      961 gccgggcgaa ttccattccg agggtcatgg ccccgaggac gagcactatg gaagaccggg
     1021 atgccagcca cacattgtcc ggccacaggt atgattgtcc aataccgagt acggccagct
     1081 gcgccaggca gatcacagcg atgtacatga cgtagatgag gtaactgcgg tcgcggatcg
     1141 aagcgaagat gaacaggttg tacagtgcca tcacgatcat gatgcccgta taccccccca
     1201 gccaaaaatt ccgggttgaa cgtgcctggg tttgcgcaga ctgcccagaa acgtacatgg
     1261 gcaaatggat ctgcttgaat ccatgcaccc gtatgaggat ggtggccgtg ctcccgggtt
     1321 ccaccggcac cgagaacgcg aactctggat catttcctac ccgctcttgc ctggaccggg
     1381 attgcccggt ccgacccaaa agccgggtgg ctccttcttc caacaggtac acatcgagtt
     1441 cgtcgatctc cgcatgactt attgaaagaa cgataccctt ctgatatgtc ccatttttga
     1501 tcgcgaacct gat
//
LOCUS       DAOQOC010000883         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67635_length_1513_cov_3.715364, whole genome shotgun sequence.
ACCESSION   DAOQOC010000883 DAOQOC010000000
VERSION     DAOQOC010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67635_length_1513_cov_3.715364"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PL106_12750"
     CDS             complement(<1..643)
                     /locus_tag="PL106_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33987.1"
                     /translation="MRPSRHIALLFALFATIALFAQPAVLVEANKFYQAGDLKSARAI
                     LDGAVKDPVIGGGPEAWVLRGFVYKDLYKAHPGDAGADALRDEALNSLYRCTVLDTAE
                     QFAKSSVQAYDYLCKTQYNDAARALNELDDVRALSLYAAYKSNVLRTDPGHDLSGRDV
                     EFNNALATVYTKRFNADREDTAWFNKATTLYHGVLAIDPDNYGANYNLATLYYN"
     gene            complement(640..1188)
                     /locus_tag="PL106_12755"
     CDS             complement(640..1188)
                     /locus_tag="PL106_12755"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNA33988.1"
                     /translation="MPSWLQKGRVLKVLLLVPLLFAATDPREADKDTTAIVQASYIYN
                     IAKLVDWKDPGMKSGTFVISVLGSSNLYQELVKKYAQKSIGKQAIEVRKLPRSAEVGT
                     CHVLFVARDELALLPAIRKTIADRNTLLVTDYPDALEDGSVVNFVVVDNVVKYEISLP
                     NARKHRLEVGSTLVQLAYVAQQ"
     gene            1217..>1513
                     /locus_tag="PL106_12760"
     CDS             1217..>1513
                     /locus_tag="PL106_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33989.1"
                     /translation="MRTGQGQSRSVRMVGIFIALLICPQPSVRSQTCDTTTLRAIERE
                     AVRAPMGYTWLEWSTRSIGHRLTGSANGRRAEESADSLFRVAGMRHVAFSPFQAT"
BASE COUNT          291 a          463 c          442 g          317 t
ORIGIN      
        1 gattgtagta caacgtcgcc aggttgtaat tggccccgta gttgtccggg tcaatggcca
       61 atacaccatg gtagagggtt gtcgccttgt tgaaccaggc cgtatcctcc cgatccgcat
      121 tgaagcgctt cgtgtacacc gtggcgagcg cgttgttgaa ttcgacgtcg cgaccggaga
      181 gatcatgccc gggatccgtt cgcaggacat tcgacttgta cgccgcatac aaggacagtg
      241 cacggacatc gtccagctcg ttgagcgcgc gcgcagcgtc gttgtactgg gtcttgcaca
      301 gataatcgta cgcttgcaca ctgctcttgg cgaactgctc cgcggtatca agcaccgtac
      361 atctgtagag gctgttcaag gcctcatcgc gcaacgcatc ggcgccggca tcgccaggat
      421 gcgccttgta caggtccttg tacacgaacc cgcgcagtac ccaggcttcc gggccgcccc
      481 cgatgactgg atccttcaca gcgccgtcca gaatggcgcg cgcgctcttg aggtcgccgg
      541 cctgatagaa cttgttggcc tccacaagca cggccggttg tgcgaacagc gcgatggtcg
      601 cgaacaatgc gaagagcaga gcgatatgcc gcgatggtct cattgttgcg ccacgtaggc
      661 cagttggacg agagtgctgc ccacctcaag ccggtgtttc cgggcgttcg gcagacttat
      721 ctcatatttc acaacattat ccaccaccac gaagttcacc accgacccgt cctccagggc
      781 gtccggatag tcggtgacca gcagggtgtt gcggtcggcg atggttttac ggatcgccgg
      841 cagcaaggcc agttcgtcgc gcgccacgaa cagcacatga caggtgccga cctcagcact
      901 ccggggcaat ttccgaacct cgatcgcctg cttgccgatg gacttctggg cgtatttctt
      961 caccagttct tggtacaggt tgctgcttcc cagcacgctg atcacgaagg tgccgctctt
     1021 catgcccgga tccttccaat ccaccagttt ggctatgttg taaatgtagc tcgcctgcac
     1081 gatggccgtg gtgtccttgt ccgcctctcg tggatcggtg gccgcgaaga gcaacggcac
     1141 cagaaggagc accttcagca cgcgcccttt ctgcagccat gaaggcatag ttttggcgcg
     1201 gaacatatcg atcggcatgc gtacgggtca gggacaaagt agaagcgtgc ggatggtcgg
     1261 cattttcatc gcactgctca tctgtccaca gcccagtgtt cgttcgcaga catgcgacac
     1321 caccacgtta cgcgccatcg agcgggaagc cgtgcgggcg ccgatgggat acacatggct
     1381 ggaatggagc acgcgctcca tcggccaccg ccttacgggt tcagccaacg gcaggcgtgc
     1441 cgaagagagc gctgatagcc tgttccgcgt ggcgggtatg cgccatgtcg cgttctcccc
     1501 gttccaagca acc
//
LOCUS       DAOQOC010000884         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67693_length_1513_cov_2.054184, whole genome shotgun sequence.
ACCESSION   DAOQOC010000884 DAOQOC010000000
VERSION     DAOQOC010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67693_length_1513_cov_2.054184"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PL106_12765"
     CDS             <1..663
                     /locus_tag="PL106_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33990.1"
                     /translation="RGVDRFVQPRDTTGPAEREDPPPQVEIIPPAVVTPAPVAAEHPA
                     LMQQSRSGRTLMYFFDLKLVHPKELYRADPVLELANGSVDARFRDARAKAEAGPPDGQ
                     IPYDRYFRSAMGKFAQGDRQGCLEDMFVVLEDYPNDVNALFYAGLCCYDLGLFARAAT
                     LLDRARSHEVDTFVEEAEWYHALARERTSGRAEAMPLFRAVVDRNGFYAARAREHLAA
                     DH"
     gene            complement(738..1007)
                     /gene="rpmA"
                     /locus_tag="PL106_12770"
     CDS             complement(738..1007)
                     /gene="rpmA"
                     /locus_tag="PL106_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNA33991.1"
                     /translation="MAHKKGEGSTQNGRESHSKRLGVKIYGGSKAVAGNIIVRQRGTQ
                     HHPGLNVGIGVDHTLYALVDGTVQFRRKRDDKSYVSVIPAEAAKN"
     gene            complement(1064..1489)
                     /gene="rplU"
                     /locus_tag="PL106_12775"
     CDS             complement(1064..1489)
                     /gene="rplU"
                     /locus_tag="PL106_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNA33992.1"
                     /translation="MVFEAEEGKHVELGNVLLVSNGSTVSVGTPVVDGARIAAKVLSH
                     GKGDKVLVFKKKRRKGYQKLNGHRQYLTELWIESILGKGEKFDASKSTAPQPKAVVAE
                     KPKAAKVKTVAPAAEKKAPAKKAAPAKKAAPKKKAAPKK"
BASE COUNT          227 a          485 c          467 g          334 t
ORIGIN      
        1 cgtggcgttg accgctttgt tcagccacgg gacaccactg gtccggccga gcgggaggat
       61 cctcctccgc aggtggagat cataccgccg gccgttgtaa cgccagcacc cgtggccgcg
      121 gagcatcccg cgttgatgca gcagtcccgg tcggggcgca cgctcatgta cttcttcgac
      181 ctgaaattgg tacacccgaa ggaactctac cgcgccgacc ccgtacttga actcgcgaac
      241 ggttccgtgg atgcgcgttt ccgtgatgcc cgggccaagg cagaggcagg gccacccgat
      301 ggccagatcc catacgaccg gtatttcagg tccgcgatgg gcaaattcgc gcagggcgac
      361 aggcagggct gcttggagga catgttcgtc gtactcgagg attacccgaa cgatgtgaac
      421 gcgctcttct atgcggggct ctgctgctac gatctgggcc tgttcgcgcg tgccgccaca
      481 ttgctcgatc gggcacgatc gcacgaggtg gacaccttcg ttgaagaggc cgaatggtac
      541 catgcgctgg cccgggagcg cacctcgggt cgcgcggaag cgatgccgtt gttccgcgcc
      601 gtggttgatc gcaacggctt ctatgcggca cgggccaggg aacacctcgc agcggaccat
      661 tgagcggcac cgcgggcctc ttccaaaaga aagccccgca gccttggccg cagggcttgc
      721 tgttcacgtt cggaagctca gttcttcgcg gcttccgcgg ggatcacgct cacgtagctc
      781 ttgtcgtcgc gtttgcggcg gaattgtacc gtgccatcca ccagggcgta cagcgtgtgg
      841 tccacaccga tgcccacatt gaggcccggg tgatgctggg tgccacgctg gcgcacgatg
      901 atgttgccgg ccacggcctt gctgccacca tagatcttca cgcccaagcg cttcgagtgc
      961 gactcgcggc cgttctgtgt actgccttca cctttcttgt gtgccatggc ttatggggtc
     1021 tgttttggat caccggtctg ctgctcccgg tggggccgat gagttacttt ttgggggccg
     1081 cctttttctt gggagcggct tttttcgccg gggcggcctt cttggcggga gcctttttct
     1141 cggcggcggg agcgaccgtc ttcaccttgg ccgctttcgg cttttcggct accacggcct
     1201 tgggttgcgg agccgtgctc ttgctggcat cgaacttctc gcccttgccc aagatgctct
     1261 cgatccacag ttccgtgagg tactgccggt gaccgttgag cttctggtag cctttccggc
     1321 gtttcttctt gaaaaccaga accttatcgc ccttgccgtg gctgaggacc ttggccgcga
     1381 tccgggcgcc gtccaccaca ggggtaccaa cgctcacggt ggacccgttg ctcacgagca
     1441 gcacgttgcc gagttcaaca tgcttgcctt cctcggcctc aaacaccatc cgcctccggt
     1501 tctacccgcg cgc
//
LOCUS       DAOQOC010000885         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_67935_length_1510_cov_3.919588, whole genome shotgun sequence.
ACCESSION   DAOQOC010000885 DAOQOC010000000
VERSION     DAOQOC010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67935_length_1510_cov_3.919588"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PL106_12780"
     CDS             complement(<1..808)
                     /locus_tag="PL106_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNA33993.1"
                     /translation="MISIHLRGPNGMERREGLAALALAAAGPERTSVVWIDLIAPDET
                     ELKDTEAFIGSELMTPKDAAEIESSSRFYEDANEIQLNAHFLQSAEKSWRPEPVTFVL
                     HGDVLVTRRTGALRSFTELERRRKNSSVTWGAHETFAALFDLRIDLDADIVEQMGREI
                     TQLNRDVNLNKAVGKELLLRIDEKLDEAMLLRANIVDKQRTLAAVLKSERFPHAYRSN
                     IRTLLRDIASLLEHIAFGFERLEFLQNTALGLLNIEQNKVIKIFTVATVVF"
     gene            960..1238
                     /locus_tag="PL106_12785"
     CDS             960..1238
                     /locus_tag="PL106_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33994.1"
                     /translation="MIWILEDSGHSPVRIIDVTLFLTNMKDDLDIQNRLWKEYFEQGP
                     SSRTPSGINGRPTPSLLAGRRSSSEVAAPRFIIRRVVMAPGGRTATSS"
     gene            complement(1225..>1510)
                     /locus_tag="PL106_12790"
     CDS             complement(1225..>1510)
                     /locus_tag="PL106_12790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNA33995.1"
                     /translation="PSALPDRISFALIDGDLYESTRHVLPHVYERMAPGAIGMIAVYY
                     DACVFPRKGIHPGYRSPGVKRATDEFFADKPEKVSLLYANEYSNGYFRKK"
BASE COUNT          300 a          436 c          480 g          294 t
ORIGIN      
        1 tgaagaccac cgtggccacg gtgaagatct tgatcacctt gttctgctcg atgttgagca
       61 gaccgagggc ggtgttctgc aggaactcca agcgttcgaa accgaaggcg atgtgctcca
      121 gcagtgaagc gatatcgcgc aggagcgtac ggatgttgct gcggtacgca tgggggaaac
      181 gttcgctctt cagcacagcg gccagggtac gctgcttgtc cacgatgttg gcacgcagca
      241 acatggcctc gtcgagcttc tcatcgatgc gcaggagcaa ttctttgcca accgccttgt
      301 tcaggttcac atccctgttc agttgggtga tctcgcgccc catctgttcc acgatgtcgg
      361 catccaggtc gatgcgcagg tcgaagagcg cagcgaaggt ctcgtgggcg ccccaggtga
      421 ccgaggagtt cttcctgcgg cgttccagct cggtgaaact acggagcgca cccgtgcggc
      481 gcgtcacgag cacatcacca tgcaacacga aggtgaccgg ttcgggacgc cagctcttct
      541 cggcgctctg gaggaaatgc gcgttcaact ggatctcgtt cgcgtcctcg tagaaccggg
      601 aggaactctc gatctccgca gcatccttgg gggtcatcaa ctcgctgccg atgaaggcct
      661 cggtgtcctt cagttcggtc tcgtccgggg cgatcaggtc gatccacacc acagaagtgc
      721 gctcggggcc tgcagcggca agggccaggg cggcaagacc ttcgcggcgc tccatgccgt
      781 tgggtccgcg caggtggatg ctgatcatgc gggggcgagt ctacgggagg agcacatggt
      841 tccatccgat cactcaacgt ggcgaaggaa caggtatacg tgcagaactg atgaaatacc
      901 gaccgtgtcc gagcgaacca tgcccgatcg ggacgcgggt acaccgcaag ttccagaatg
      961 tgatatggat cctggaggac agtgggcaca gcccggtgcg gatcatcgac gtcacgctgt
     1021 tcctcaccaa catgaaggat gatcttgaca tccagaaccg gttgtggaag gagtacttcg
     1081 agcagggccc ttcttcccgc accccatcgg ggatcaatgg ccgacccacc ccatcgctgc
     1141 tggctggaag gcgatcgtcc agtgaagtgg ccgcaccacg cttcatcatt cgaagggtgg
     1201 tgatggcacc aggcgggcga acagctactt cttcctgaaa tagccgttcg aatactcgtt
     1261 cgcgtagagc aaggatacct tttcgggttt gtcggcgaag aactcgtcgg tggcacgctt
     1321 cacgccgggc gagcgatagc cgggatggat gcccttgcgc gggaacacgc acgcgtcata
     1381 gtacacggcg atcatgccga tggcgccggg cgccatgcgt tcgtacacgt gcggcagcac
     1441 atggcgcgtg gattcgtaca ggtcaccgtc gatgagtgcg aagctgatcc ggtcaggcag
     1501 ggccgatggc
//
LOCUS       DAOQOC010000886         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_68288_length_1506_cov_3.922123, whole genome shotgun sequence.
ACCESSION   DAOQOC010000886 DAOQOC010000000
VERSION     DAOQOC010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68288_length_1506_cov_3.922123"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..912)
                     /locus_tag="PL106_12795"
     CDS             complement(4..912)
                     /locus_tag="PL106_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNA33996.1"
                     /translation="MKYTTLTCLTGLLLFTACEKNKDRSDASGTFEATETIVSAEVNG
                     KVMAFTAEEGDQVKAGQVLGYIDSTQLYLQKLQLQQNRRAVLAGQPNVATQLEALEKE
                     LDHALSDRGRVANLVKGDVASKKQLDDADTRIAVLRARIAAQRNELGTRNTALYEQGR
                     TIEVQLRSVEDQLGKCRIESPLDGTVLTTYVNPNEMTAAGRPLFKVADLGTMRLKAYL
                     TGDQFDNVKLGQAVRVLVDSGDGGTKEYPGEVEWISDRAEFTPKTIQTRDERSNLVHA
                     IKVRVKNDGLIKIGMYGEVILENQGS"
     gene            complement(929..>1506)
                     /locus_tag="PL106_12800"
     CDS             complement(929..>1506)
                     /locus_tag="PL106_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA33997.1"
                     /translation="RPAVREDFASAPDQRPELGTFTAQRELATAQYQLGKTSLMPKVG
                     LLGTGLLFAPALGFGTSEISSLALAGISLSWNTSGLYRQANDRKLHELHLARIANEES
                     SFRFANGLDLRRDDQEVRKARAILAADEGIVALRTRIADAYQLKYDNGLCAMNDLIDA
                     MSEVDEARSDKALHEIQLLLAMQHYATDNGN"
BASE COUNT          229 a          478 c          449 g          350 t
ORIGIN      
        1 cattcatgag ccctggtttt cgaggataac ttcgccgtac atgccgatct tgatcaggcc
       61 atcgttcttc acgcgcacct tgatggcatg gacgaggttg ctccgctcgt cccgtgtttg
      121 gatcgtcttg ggcgtgaact ccgcgcggtc gctgatccat tccacctcgc ccggatactc
      181 cttggtgcca ccatcgccgc tgtccaccag cacccgcacg gcctggccga gcttcacgtt
      241 gtcgaactgg tcgccggtga ggtaggcctt gaggcgcatc gtccccaggt cggccacctt
      301 gaacaacgga cggcctgccg cggtcatttc attcgggttc acataggtgg tcagcacggt
      361 gccgtccaat gggctttcga tccggcattt gccgagctgg tcctccacgg aacggagctg
      421 cacctcgatc gttcttccct gttcgtagag cgcggtgttc cgcgtgccca gttcgttgcg
      481 ttgtgcggcg atccgtgcgc gcagtaccgc gatgcgcgta tcggcgtcgt caagttgttt
      541 cttcgatgcc acatccccct tcaccaggtt cgctacacgc cccctgtcgc tgagtgcatg
      601 gtccaattcc ttctccagcg cctcgagctg ggtggcgacg ttcggctggc cggcaagcac
      661 cgccctgcgg ttctgttgca actggagttt ttggaggtac agttgcgtgc tgtcgatgta
      721 gcccagcact tgtccggcct tcacctggtc gccttcctcg gcggtgaagg ccataacctt
      781 cccgttcacc tcggcggaga cgatcgtttc cgtggcctcg aaggtgcccg acgcatcgct
      841 ccggtccttg ttcttttcac aagccgtgaa aagcagcaaa ccggtgaggc aggtgagtgt
      901 ggtgtacttc atggtgggtt gttggcgtct agttcccatt gtcggtggca tagtgctgca
      961 tggccagcag caattggatc tcgtgcaggg ccttgtcgct gcgcgcctca tccacttcgc
     1021 tcatggcgtc gatcaggtcg ttcatggcac acagcccgtt atcatacttg agctgatagg
     1081 cgtcagcgat ccttgtccgg agcgccacga tcccctcatc ggccgccagg atcgcacggg
     1141 ccttccgcac ctcctggtcg tcccgccgca ggtcgagccc gttggcgaat cggaaactgc
     1201 tctcctcgtt ggcgatccgc gccaagtgca gctcatgcaa cttgcggtcg ttggcctgcc
     1261 gatacagccc cgaggtgttc catgaaaggc tgatcccggc cagcgccaag gatgaaattt
     1321 cggaagtgcc gaagcccagc gctggtgcaa agaggaggcc cgtgccgagc agtccgacct
     1381 tgggcatgag cgaggttttt cccaactggt attgcgccgt cgccagttcg cgctgcgctg
     1441 tgaacgtgcc cagttccggc cgttggtccg gtgcggaagc aaagtcctcg cgtaccgcag
     1501 ggcgtt
//
LOCUS       DAOQOC010000887         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_68394_length_1505_cov_2.820000, whole genome shotgun sequence.
ACCESSION   DAOQOC010000887 DAOQOC010000000
VERSION     DAOQOC010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68394_length_1505_cov_2.820000"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PL106_12805"
     CDS             <1..291
                     /locus_tag="PL106_12805"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNA33998.1"
                     /translation="PSLTRDLLLQSFGIGSQLGMLAYSRSHESEADKMGLVFMAMAGY
                     DPRGAPEFWKRMAAQGGPKPPELLSTHPNDEKRVADLEAYMPEAMKYYKPRP"
     gene            380..456
                     /locus_tag="PL106_12810"
     tRNA            380..456
                     /locus_tag="PL106_12810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:414..416,aa:Arg,seq:acg)
     gene            609..>1505
                     /gene="ychF"
                     /locus_tag="PL106_12815"
     CDS             609..>1505
                     /gene="ychF"
                     /locus_tag="PL106_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNA33999.1"
                     /translation="MKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGTITV
                     PDTRLNKLADLVKPQRIVPTTVEIVDIAGLVKGASKGEGLGNQFLANIRECDAILHVL
                     RCFEDPNVVHVDGSVDPVRDKEVIDIELQLKDLETVEARIKKVEKQAQIGEKEAKRRY
                     DLLTRIRTALLKGESARAVITPSDDPALLNEFQLLTSKPVMYVCNVDEASAVKGNAFV
                     EKVKAAVAGENAEVIFVTAAIEAEIASLETPEEREMFLTDMGLEEPGVNKLIHAAYHL
                     LKLQTYFTAGVKEVRAWTIHQGD"
BASE COUNT          321 a          454 c          477 g          253 t
ORIGIN      
        1 ccaagcctga cgcgcgacct gctgctgcaa tccttcggca tcggcagcca actcggtatg
       61 ctggcgtaca gccgcagtca cgagagcgag gccgacaaga tgggtctggt cttcatggcc
      121 atggccggct acgatccacg cggagccccc gagttctgga agcgcatggc cgcacaaggc
      181 ggtcccaagc cgcccgaatt gctgagcaca caccccaacg acgagaagcg tgtggccgat
      241 ctggaagcgt acatgcccga ggccatgaag tactacaagc cgcgtccttg accgaccaca
      301 gacctggttc gccggatcgg aggtgaaagg aagtgcgtca ttcaaaggag gatgactaca
      361 ttcgcgccca ctttttcagg gacccgtagc tcaattggat agagcatctg actacggatc
      421 agaaggtttg gggttcgact ccctacgggt ccgccaagcg gaaggctctc cacctcgggg
      481 ggcctttcgc gtttcaaggg gtagctggca ttgccgcccg gcaagagacg gctattttcg
      541 cggccctttt ggcccgacct cgcctcgcca tgttggtgag cgctgccggg cgtttgaaca
      601 ccatgggatt gaaatgcggt atcgtcggcc tgcccaacgt gggcaagagc accctgttca
      661 actgcctcag caacgccaag gcgcaagcgg cgaacttccc gttctgcacc attgagccca
      721 acgtgggcac catcaccgtg cctgataccc ggctgaacaa actggccgac ctggtgaagc
      781 cccagcgcat cgtgcccacc accgtggaga tcgtcgacat cgcgggcctg gtgaagggcg
      841 ccagcaaggg cgagggcctg ggcaaccaat tcctggccaa catccgcgag tgcgatgcca
      901 tcctgcatgt actgcgctgc ttcgaggacc ccaacgtggt gcatgtggac ggtagcgtgg
      961 atcccgtgcg cgacaaggag gtgatcgata tcgaactcca actgaaagac ctggagaccg
     1021 tggaggcccg gatcaagaag gtggagaaac aggcccagat cggcgagaag gaggccaagc
     1081 ggcgctacga cctgctgacc cggatccgga ccgcattgct gaaaggcgaa agcgcacgtg
     1141 cggtgatcac cccctccgac gacccggcct tgctgaatga gttccaactg ctcacctcca
     1201 agccggtgat gtacgtgtgc aacgtggacg aggcgagcgc ggtgaaaggc aatgcgttcg
     1261 tggagaaggt gaaggccgcc gtggccggcg agaacgcgga ggtcatcttc gttaccgccg
     1321 cgatcgaggc ggagatcgcc agcttggaga ccccggagga gcgcgagatg ttcctgaccg
     1381 atatgggcct ggaagagcct ggcgtgaaca agctgatcca cgccgcctat cacctgttga
     1441 agctgcagac ctatttcacc gccggggtga aggaggtgcg tgcatggacc atccaccaag
     1501 gcgat
//
LOCUS       DAOQOC010000888         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_68561_length_1503_cov_3.038674, whole genome shotgun sequence.
ACCESSION   DAOQOC010000888 DAOQOC010000000
VERSION     DAOQOC010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68561_length_1503_cov_3.038674"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PL106_12820"
     CDS             complement(<1..996)
                     /locus_tag="PL106_12820"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNA34000.1"
                     /translation="MFKVLFDIGHPGHVHLFKHLARLLMVDGAQVLFTSRVKEHEMAL
                     IAHEGFDHVSFGPHYRSLTGKLWGLLRYDLAMLRTGLRFRPDLIVGHGTIYGAHASPL
                     LRAKCLAIEDTGNMEQVRLWRPFVDAILTPKVLNKDLGPKQVRYDSYHELAYLHPEFY
                     MPDPGILDWLKVAPDEPYAILRFVSWNATHDVGLKGLSVDEKVELVRRLSGRMKVFIT
                     SERGVPSELEPYVMKIPPERFHHALRFARIVITEGTTTGAEAAVLGVPVVFVSTYRAA
                     HLHEMEGFGLVANTTNGPDAFSAIERMLLLDPHEYRERAAKFIATKVNATRFYHRF"
     gene            complement(1002..>1503)
                     /locus_tag="PL106_12825"
     CDS             complement(1002..>1503)
                     /locus_tag="PL106_12825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA34001.1"
                     /translation="WGLAANGDEVREFYRILHGLYRSKVRKPLPPLELFLQLHAHPDG
                     RLFVVRHEGRVIGGCAAPVYRDRMIHYWYVCGDNSAKGIQASVLATWASIAHAAQQGI
                     GTLDFMGAGKPGQGYGVHEFKARFGGIEVEHGRYETVLEPALYRMGAAGLKVYQHIRP
                     LFHKDR"
BASE COUNT          314 a          472 c          439 g          278 t
ORIGIN      
        1 gaaccggtgg tagaaacgag tagcgttcac cttcgtcgct atgaacttcg cagcgcgttc
       61 acggtactca tgcggatcca gaagcagcat tcgctcgatc gctgagaacg catccggtcc
      121 gttggtggtg ttggccacca ggccgaagcc ctccatttcg tgcaaatgtg cggcgcgata
      181 ggtgctcacg aaaaccacgg gcacgccaag tacagcggcc tcggccccgg tggtggtccc
      241 ttcggtgatc acgatacgtg cgaaccgcaa cgcatggtgg aaccgctccg gtggtatctt
      301 catcacatag ggctccagct cggacggaac accgcgttcg gaggtgatga aaaccttcat
      361 acgcccggat aaccgacgca ccagctccac cttttcatcc acggacaagc ctttcagtcc
      421 aacgtcgtgg gtagcattcc acgaaacgaa ccgcaggatg gcgtacggct catccggcgc
      481 caccttcaac cagtccagta tcccgggatc gggcatgtag aactccggat ggaggtaggc
      541 cagttcatga taactgtcgt aacgcacctg cttcgggccg aggtccttgt tcagcacctt
      601 gggggtcagg atcgcatcca caaagggtcg ccagaggcgt acctgttcca tgttgccggt
      661 gtcctcgatg gccaggcact tcgcacggag cagtggcgac gcgtgcgctc cgtaaatcgt
      721 accgtggcct acgatcagat ccggacggaa gcgcagtccg gtgcgcagca tggccaggtc
      781 gtagcgcagg agcccccaca gctttcccgt cagcgaccgg tagtgcgggc cgaaactcac
      841 atggtcaaag ccctcgtgcg cgatgagcgc catctcgtgc tccttcaccc gcgaggtgaa
      901 cagcacctgg gcaccatcga ccatgaggag gcgcgccaga tgtttgaaga ggtggacatg
      961 cccagggtgg ccgatatcga acaatacctt gaacatggtg ctcaacggtc cttgtgaaaa
     1021 agcggccgga tatgttggta caccttcaaa ccggctgcac ccatgcgata gagggcgggt
     1081 tcgagcaccg tttcatatcg cccgtgctcc acctcgatcc ccccgaaacg cgccttgaac
     1141 tcgtgtacac catagccctg gccgggtttc cccgcaccca tgaagtcgag tgtaccgata
     1201 ccctgctgtg cagcatgggc gatggacgcc caggtagcga gcacactggc ctgaatgccc
     1261 ttcgcgctgt tgtcgccgca gacataccag taatggatca tacggtcgcg gtatacgggg
     1321 gctgcgcaac caccgatcac ccgcccctcg tggcgcacca cgaacaggcg accgtccgga
     1381 tgcgcgtgca gttggaggaa aagctccagg ggtggcaagg gcttgcgcac tttggaacgg
     1441 tacagtccat gcaggatccg gtagaattca cgcacctcat ccccgttcgc ggccaacccc
     1501 cac
//
LOCUS       DAOQOC010000889         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_68918_length_1499_cov_4.351108, whole genome shotgun sequence.
ACCESSION   DAOQOC010000889 DAOQOC010000000
VERSION     DAOQOC010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68918_length_1499_cov_4.351108"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PL106_12830"
     CDS             complement(<1..1422)
                     /locus_tag="PL106_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34002.1"
                     /translation="MLLDGTVTGTQANCAPANVQWSITPGTYTPVVLPNSADISVQFQ
                     EPGTYQVQMSVTTPACGDSVWTDVICVESPPDPSFSLVLASDCAPTNATVAFDTPNTE
                     DCPDPMEFLWSVSAFGNNPCQDTGTFAISDPDGPVPGISFSGPGTYLVDLDVEDPGCP
                     VQSAQQTVTVHGPPEPLAFTVDQDTICEGDPVVVSGTVQNCDQGSPAINWTIENGTPA
                     SFTGANPGPISFSGTTPGQPNTITVTATNGCPGTVSQDIDIWVVPASPPIDPQVDVLQ
                     ICPGDPVEVTFTASPGVTYGWAITGTALSGSNVSSPFTITGTDGLLPDDYTIQITSDN
                     AGCGAADASVNFQVQQAESLLIIGPEAVCEGAEVNLTVTGATNPQWSINGVQGGTGNS
                     MTDVPAGTTTYAVTSAGVPGSCPGQAELVVSTIAYPVPGFDLPGTPCAGSPATLDNTT
                     AGAFDWEWLVDGNGVPNNTVEDLV"
BASE COUNT          283 a          435 c          490 g          291 t
ORIGIN      
        1 caccagatcc tccacggtgt tgttcggcac accgttgcca tccacgagcc attcccaatc
       61 aaaggccccg gccgtggtgt tgtccaaggt tgcgggcgat cccgcgcaag gggtaccagg
      121 gaggtcgaaa ccgggtacgg gataggcgat ggtactcacc acaagctctg cctggccggg
      181 acacgagccc ggtaccccgg cagatgtgac cgcgtacgtc gtggtaccgg caggcacatc
      241 ggtcatgctg ttccccgtgc ccccctgaac accgttgatg ctccactgcg ggttggtggc
      301 tcccgttacg gtaaggttga cctcagcgcc ctcacaaacg gcctcgggcc cgatgatcag
      361 caggctttca gcttgttgca cctggaaatt gacgctggcg tcggccgcac cacaaccggc
      421 attgtccgag gtgatctgga tcgtatagtc atccggaagc aagccgtccg tacccgtgat
      481 ggtgaacggg ctgctgacgt tgctcccgga aagtgcggtg ccggtgatcg cccatccata
      541 ggtgacaccc ggcgaagcgg tgaaggtcac ttcgaccggg tctcccgggc agatctgcaa
      601 cacatccact tgtgggtcga tcggaggcga ggcgggcacg acccagatgt cgatatcctg
      661 actgaccgtg ccggggcatc cattggtagc ggtaacggtg atggtattgg gttgcccggg
      721 tgtggtacca ctgaaggaga tggggcccgg gttcgcaccg gtgaaactgg ccggagttcc
      781 gttctcgatg gtccaattga tggcggggct tccttgatcg caattctgga ccgtaccgct
      841 cacgaccacc ggatccccct cgcagatcgt gtcctggtcc acggtgaagg ccaacggctc
      901 cggaggtcca tggacggtga ccgtttgttg ggcgctctgg accggacagc cggggtcctc
      961 cacatccagg tccaccagat atgtacctgg gccggagaac gaaataccgg gcaccggccc
     1021 atccgggtcg gaaatggcga aggttccggt atcctggcag gggttgttgc cgaaggccga
     1081 aacactccag agaaattcca tcgggtccgg gcaatcctcc gtgttcggcg tgtcgaaggc
     1141 gacggtcgcg ttggtgggtg cgcagtcgga ggccagcaca aggctgaagg atggatccgg
     1201 agggctttcc acgcagatca catcggtcca taccgaatca ccgcatgccg gtgttgtcac
     1261 ggacatctgc acctgatacg tacctggttc ctggaattgg accgagatat ccgctgaatt
     1321 gggaagcacc acaggggtgt aggtaccggg cgttatggac cattgcacat tggccggcgc
     1381 acagttggcc tgggtaccgg tcacggttcc atcgaggagc acgttggtat cctcgcaaac
     1441 cggatcctga tgggtgaaga atgcgctggg ggctcccagg acggtgacgg aagtggtgt
//
LOCUS       DAOQOC010000890         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_68927_length_1499_cov_3.528393, whole genome shotgun sequence.
ACCESSION   DAOQOC010000890 DAOQOC010000000
VERSION     DAOQOC010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68927_length_1499_cov_3.528393"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PL106_12835"
     CDS             complement(<1..771)
                     /locus_tag="PL106_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNA34003.1"
                     /translation="MLFSTSVAHGQDDKALYGTGEKVFKQNCASCHKPTEKMTGPPIK
                     GAKEKWASTGGDIYAWVKNSQAYLKANPGDQHAHDLLKEYGGSVMTPNAISDEEIDAV
                     LYYADNFVGKAKAPEAAGGATAEAEGSDNQTSWQWLIIVVLLLLIVVLSLSGVKNQLQ
                     NALNEREGKPPVPVRSVWGNIWHWAVTHRSWALIFGFLFVCWLMVLQWDYLWNIGVYG
                     GEEVPHYKPEQPIAFNHSLHAGKMAINCQYCHSGAEKSK"
     gene            1061..>1499
                     /locus_tag="PL106_12840"
     CDS             1061..>1499
                     /locus_tag="PL106_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34004.1"
                     /translation="MIRCLLFLLAGPATVQTVHAQVDPRLFVPHHQQVEANAPDTGGI
                     TLHEPEGVTELMKTYAATKQPLKGYRVQIFLGERDKAEETRRAFLVRHPDVPAYLSYL
                     APNFRVRVGDQRTKLEGEKLRDHLATEYPGLYVVPDAIEMPRLG"
BASE COUNT          292 a          458 c          442 g          307 t
ORIGIN      
        1 tttgctcttc tccgcaccgc tatggcaata ctggcagttg atggccattt tgccggcgtg
       61 cagggaatgg ttgaaggcga tcggttgctc gggtttgtag tgcggcacct cctctccgcc
      121 gtacacaccg atgttccata ggtaatccca ctgcagcacc atgagccagc agacgaagag
      181 gaaaccgaag atcagggccc aactccggtg cgtcacggcc cagtgccaga tgttgcccca
      241 cacgctgcga acgggtaccg ggggcttgcc ctcgcgctcg ttcagtgcgt tctgcagctg
      301 gttcttcacc ccgctgagcg acagtacaac gatcagcagc agcagcacca cgatgatcaa
      361 ccactgccag gacgtctggt tgtccgatcc ttcagcctct gctgtggcac caccagcggc
      421 ttccggtgcc ttggccttgc ctacgaagtt gtcggcgtag tacaggacgg catcgatctc
      481 ctcgtcgctg atggcattgg gcgtcatcac gctgccgcca tactccttca agaggtcatg
      541 ggcatgctga tcacccgggt tggccttcag gtacgcctgg ctgttcttca cccaagcata
      601 gatgtcgcct ccggtactgg cccatttctc cttggcccct ttgatcgggg gaccggtcat
      661 cttctcggtg ggcttgtggc agctggcgca gttctgcttg aaaaccttct cccctgttcc
      721 atacagcgcc ttgtcgtcct gaccgtgcgc cacggaggtc gaaaacagca acagaacggc
      781 gaaacacgcg ttgaggagcc cggtggaaac ggctgaaagc cttgatggaa gcggcattcc
      841 gggaaatttg ggcgctcacc gcaacgagcg agcgtggcgg caaaaataga agccggtgtt
      901 tatggtggac ggacaacagt aaattgtcat gcccaggtca tctatttaga atgattccaa
      961 agatgtccgg gtccgagata tgcgcttcgg gaatgcgtga tgaacacgtt tccgggacga
     1021 tcgggtgtga gcaccgtcct acctttggcc tttacggctc atgatccgat gtctcctgtt
     1081 ccttttggcc ggcccggcaa cggtccagac ggtccatgca caagtcgatc cccggctatt
     1141 cgtgccgcac caccagcagg tggaggccaa cgcgcccgat accggtggga tcaccctgca
     1201 cgaaccggaa ggggtgaccg aactgatgaa gacctacgcg gcgaccaaac aacccctgaa
     1261 gggctaccgc gtgcagatct tcttgggtga acgggacaag gcggaggaaa cacgtcgcgc
     1321 ttttcttgtg cgccacccag acgtgccggc ctacctcagc tacctggccc ccaacttccg
     1381 cgttcgcgtg ggcgatcagc gcaccaagct cgagggcgag aagctgcgcg accacctggc
     1441 caccgagtac cccggcctat atgtggtgcc ggacgcgatc gagatgccgc ggttggggg
//
LOCUS       DAOQOC010000891         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_69207_length_1496_cov_4.114504, whole genome shotgun sequence.
ACCESSION   DAOQOC010000891 DAOQOC010000000
VERSION     DAOQOC010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69207_length_1496_cov_4.114504"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PL106_12845"
     CDS             <1..858
                     /locus_tag="PL106_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34005.1"
                     /translation="PWNVAIPEVAKDIITHQGAGLAGALRPARMRKLERRYCGPADVL
                     TAVSKTFLANAMMITGHRDGHVIHGGSVRARITRTTDPQGKFILAHTGQLYPEQPWPL
                     FFDAITRLQQEAPDLAAQLVVRFVGATSTDLSTMHLLERAERGSGLIERMPTVTPDEA
                     IRLQTEADALLQLALTGRKGYLPVKFLEYLGAGKPIILLSAEEDEMESALTSTRTGAI
                     VRDARQLVAHLIAHLEAHRTGHSLKYAPDERALSAFDYATNMDRWADLLGSALRTRTD
                     RATEPTSVR"
     gene            928..>1496
                     /locus_tag="PL106_12850"
     CDS             928..>1496
                     /locus_tag="PL106_12850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA34006.1"
                     /translation="MSTSTNRPLHVMHIFATSGSVPYLTWFAQHAQRDGDVRLSFLVM
                     YHERPAMMDEMPAMGFDCHWIRYDPRQRKRGLLKALPWLWWHMMRARPDVVHGHLFDD
                     TLPGMIAAWFARIRLRIYTRQDSGYHWNYAPRWVFLDRLVARFAHKAVACSNDVRAHM
                     IGKERVDPAKVAMVHHGIPPARYTVRDEAT"
BASE COUNT          289 a          480 c          454 g          273 t
ORIGIN      
        1 ccctggaacg tggccattcc cgaggtggcg aaggacatca tcacccacca aggcgcaggg
       61 ctggcggggg cgttgcgccc cgcgcggatg cgcaagctcg aaagacgtta ctgcggaccc
      121 gccgatgtgc tcaccgccgt gagcaagacc tttctcgcca atgccatgat gatcacgggc
      181 catcgggatg gtcatgtgat ccacggtgga tccgtccgag cgcgaataac acgaacaacg
      241 gaccctcagg gaaaattcat cctggcccac accggtcaac tgtatccgga gcaaccatgg
      301 ccgctcttct tcgatgcgat cacccggctg cagcaagagg ccccggacct ggccgcacaa
      361 ttggttgtcc gctttgtggg agctaccagt acggacctct cgaccatgca cctgctcgaa
      421 cgggcggaaa gaggatcagg gctgatcgaa cgcatgccca cggtgacgcc cgatgaggcc
      481 atccggctgc aaacggaggc ggatgccctg ttgcaattgg cgctcaccgg gcgtaagggc
      541 tatctccccg tgaagttcct cgaatacctc ggcgcaggaa aaccgatcat cctcctttcc
      601 gctgaagagg acgagatgga gagcgcattg acgtccacac gcaccggcgc gatcgtcaga
      661 gatgcccggc aactggtggc gcacctcatc gcacacttgg aggcgcaccg caccgggcat
      721 agcctgaagt acgcaccgga tgaacgtgcc ttatcagcct tcgactacgc caccaacatg
      781 gaccggtggg ccgacctgct gggatcggca ctccgcacga ggaccgaccg ggccactgag
      841 ccaacttcgg tccgttgatc gaacggcagc tgttttcccc tgttgatacg ctggacccgt
      901 tcctttgtgg cccggctcct tccttccgtg tccacgtcca ccaaccgtcc gctgcatgtg
      961 atgcacatct tcgccacgag cggttcggtg ccctacctca catggttcgc gcaacacgca
     1021 caacgcgacg gggatgtgcg tctttccttc ctggtgatgt accacgagcg accggccatg
     1081 atggacgaga tgcccgcgat gggtttcgac tgccattgga tccggtacga tccacggcag
     1141 cgcaaacgcg gactgctgaa ggccttgccc tggctgtggt ggcacatgat gcgtgcacgg
     1201 ccggatgtgg tgcacggcca tctgttcgac gacacacttc cgggcatgat cgccgcctgg
     1261 ttcgcgcgga tcaggttgcg catctatacc cggcaggatt cgggctacca ctggaactat
     1321 gctccgcgct gggtgttcct ggaccggctc gtggcgcgtt tcgcccacaa ggccgtggcg
     1381 tgttcaaatg atgtgcgcgc gcacatgatc gggaaggagc gcgtggatcc ggccaaggtg
     1441 gcgatggtcc atcacggaat accgcccgcc aggtacacgg tacgcgatga agccac
//
LOCUS       DAOQOC010000892         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_69757_length_1491_cov_2.212396, whole genome shotgun sequence.
ACCESSION   DAOQOC010000892 DAOQOC010000000
VERSION     DAOQOC010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69757_length_1491_cov_2.212396"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(581..1255)
                     /locus_tag="PL106_12855"
     CDS             complement(581..1255)
                     /locus_tag="PL106_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNA34007.1"
                     /translation="MCQAAYDADEPILVDAEDSWIQPAIDELVERMMERFNRERAIVF
                     NTTQHYRHDRLEFLRTARAKAEKGGYHYGVKLVRGAYMEKERARAAEKGYPDPIQPDK
                     AASDRDYDAAVRFCIDHIDRISVCVGTHNEASSLLTARLMAGRGLDKKDKRIWFAQLL
                     GMSDHISYNMAAAGYNVVKYVPYGPVRKVMPYLIRRAIENTSARGQTGRELGLILTEI
                     RRRRKQ"
     gene            1277..>1491
                     /locus_tag="PL106_12860"
     CDS             1277..>1491
                     /locus_tag="PL106_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA34008.1"
                     /translation="MDFADLEQFALRLLWDATARKPTPLAQSWRARLDLVLVDEYQDI
                     NGAQDRILACVSREGLAANRFLVGDVK"
BASE COUNT          250 a          472 c          475 g          294 t
ORIGIN      
        1 acgcccaacg ggaagaacgt cacccggttc gcccgttcga aggaactgag ggtctacgcg
       61 ccggcacgcc cgccggcatt cacccgcagg tgctcattgt agcacaatgc cccgtcgaac
      121 aggtcgatat ggtcccccgc actgtcgtta tctcggacaa atatgtcgtt aaaatagaga
      181 ataacgcaac gaccacccgg ttcctcggca cccggaacag gggcctcacc gcatctgcag
      241 ctccttttct ccgcgctgtg cacacgtccg ggtccgggga agtccgccag ccccaagcca
      301 caccgcccgg ggccacgctc atgcgcacgg cgcactggtt ggtggcaccg cccttacagg
      361 gtgcactgcc cgtgaggtac gcgtcgtagc agtgcttcag gaagaggcac gaagggatgc
      421 tcaccggttc ctggaccagg acggcatatc gatcgatgag gtcggccatg atcgatcgct
      481 tgtcacatcg caaaggggat cagcggcccg ctgaagggca atccataccg cgtgatcgtc
      541 ggtttttatc gatcggttgg acgtgggggc gggcctgcgg tcactgcttc cggcggcgcc
      601 ggatctccgt caggatcaat cccagttccc tgcctgtctg cccacgggca ctcgtgttct
      661 cgatggctcg ccggatcagg tagggcatca ccttgcgtac cggaccatag ggaacgtatt
      721 tgaccacgtt gtacccggcc gcggccatgt tgtagctgat gtggtcgctc atcccgagca
      781 actgggcgaa ccagatccgc ttgtccttct tgtccaggcc gcgtccggcc atgagccggg
      841 ccgtgagcaa cgagctcgct tcattgtgcg tgcccacgca tacggagatc cggtcgatgt
      901 gatcgatgca gaagcggacc gcggcatcat aatcgcgatc actggcggcc ttgtcgggct
      961 ggatggggtc ggggtatccc ttttccgctg cccgcgcacg ttctttctcc atgtatgcgc
     1021 cgcgcaccag tttcacaccg tagtggtacc cgcctttttc ggctttggcg cgcgcggtgc
     1081 gcaggaactc cagacgatcg tgccggtagt gctgggtggt gttgaacacg atggcgcgtt
     1141 cccggttgaa gcgttccatc atgcgctcca ccaactcgtc tatcgcgggc tggatccagc
     1201 tgtcctcggc atccaccagg atgggctcat cggcatcgta tgcggcctgg cacaggcgaa
     1261 gcgggcgctt ggcgtcgtgg atttcgcgga cctcgagcag ttcgcgctgc ggctgctgtg
     1321 ggacgcgacc gcgcggaagc ccacgccgct ggcgcagtcg tggcgcgcgc ggctcgacct
     1381 cgtgctggtg gacgaatacc aggacatcaa cggtgcgcag gaccggatcc tggcgtgcgt
     1441 cagtcgcgag ggtttggcgg cgaaccggtt tctcgtcggc gacgtgaagc a
//
LOCUS       DAOQOC010000893         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_69787_length_1490_cov_3.597213, whole genome shotgun sequence.
ACCESSION   DAOQOC010000893 DAOQOC010000000
VERSION     DAOQOC010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69787_length_1490_cov_3.597213"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PL106_12865"
     CDS             complement(<1..191)
                     /locus_tag="PL106_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34009.1"
                     /translation="MAVRPTLSVAGSLMLTIVTSAQNPLAIPPLIEQDTFQLVLDEGT
                     KEFYPGVVTDTYGISAPFLG"
     gene            complement(870..>1490)
                     /locus_tag="PL106_12870"
     CDS             complement(870..>1490)
                     /locus_tag="PL106_12870"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNA34010.1"
                     /translation="FITFLAGLLLVAYGLIRALRSRKPDLWMANGGMIAALGFIGFSR
                     WPEAAIPAGMDQQSAAFMGAWWGGWLAAVWASYQLVWSLRALTWPSVQGTLTQSEAVF
                     MGINNSTTIGPRGSTKHWWKVAYTYSVDGVNYSGTNKALDTDDQDSNFSGVSKKVAEH
                     PPGSTIRVYHHPRDPQLACIDRGWFNGRWLAPAMYAVVLFWVAGLE"
BASE COUNT          280 a          443 c          468 g          299 t
ORIGIN      
        1 cccaggaagg gcgcgctgat gccgtatgta tccgtcacca caccggggta gaattccttc
       61 gtgccctcgt ccagtaccag ttggaaggtg tcctgctcga tcaagggcgg gatggccagc
      121 gggttctggg cggaggtaac gatggtcagc atcagggagc ctgcgacgga taaagtgggg
      181 cgtactgcca tgggcgtggt ctttgcgggc aagctgtgct tttcggaaag agcggaacat
      241 gaccttcatc aggtggtgcc tgccgcaatg atcgtaggcc atgttgatcc tgcacacgtc
      301 cggatgggtc gctcaggacg atcgaccggc atgcccgtcg aagtcgtgga tcggccagtt
      361 cccggatccg aaccctgatc tgcgaggatg cgtgccgcga cttcggtccg ggtggtccag
      421 cgatgcaaat acttcgtggc agaggcagca cgacgtggcc tggtcattta ttccgctcac
      481 gtatcgacgt cagttgctat tggaagcgga agggtgggta tttgatcgcc gttcgcttcg
      541 atcacgtgac cgctgcacac ttcttcgcct catcgcccgg ggggcatcat gcccaactgc
      601 tgcatcgtct tcatttgtct ttgctgtgct atcggccgcc atcatggcca tgtggtcggc
      661 catgatcttg tcgtgctccg ggttggaccc accgccgcag gccgttgaca tggtcaaagg
      721 gaaggcgaga agaacgttct atttcatgtt gtcgtgtgtt ggcgaaaagt gactgaacgc
      781 aacacaccgg taccgatgat acaagcgacc ggatcgcgag cgatggtccg gcaggatcga
      841 tcccgggcgg aaggatcctc agcctgtgct cattcaaggc cagcgaccca gaacagcacc
      901 acagcgtaca tcgctggtgc cagccatctc ccattgaacc agccccgatc gatgcaggcg
      961 agctgcggat cacgtggatg gtggtacacc ctgatcgtcg agcccggagg atgctccgcg
     1021 acctttttgc tcacgccgct gaagttgctg tcctggtcgt cggtatccag ggccttgttg
     1081 gtgcctgagt agttcacgcc gtcgacagag tacgtgtagg ccaccttcca ccagtgcttg
     1141 gtgcttccgc gcggtccgat ggtggtggag ttgttgatgc ccatgaacac cgcctcgctc
     1201 tgggtgagcg tgccttggac cgacggccac gtcaatgcgc gcagcgacca gaccagttgg
     1261 tacgaggccc acacggccgc gagccagcca ccccaccatg cacccatgaa ggcagcgctc
     1321 tgctggtcca tgccggcagg gatcgccgcc tcaggccaac gactgaaccc gatgaacccg
     1381 agggccgcga tcatgccgcc gttcgccatc cacaggtccg gcttgcggga ccgcagcgct
     1441 cggatcaggc cataggccac cagaagaagg cccgcgagaa aggtgatgaa
//
LOCUS       DAOQOC010000894         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_69938_length_1489_cov_1.835425, whole genome shotgun sequence.
ACCESSION   DAOQOC010000894 DAOQOC010000000
VERSION     DAOQOC010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69938_length_1489_cov_1.835425"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PL106_12875"
     CDS             complement(<1..348)
                     /locus_tag="PL106_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34011.1"
                     /translation="MFPSRPLLLIGTAISCASADCATISVTYNFAPQEAQVAIDHAAS
                     IWEGILVSPVPIKAMVTWIPMNGSVLGITFPNGRKDFTGAPVPQTWYATALANSIAGQ
                     ELNPGENDIEIYLD"
     gene            complement(365..1339)
                     /locus_tag="PL106_12880"
     CDS             complement(365..1339)
                     /locus_tag="PL106_12880"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNA34012.1"
                     /translation="MKPARIALLTSGGDAPGMNAAIRAVVRTAAFHGIPVSGVMDGFD
                     GLVKAHFIELGPRNVRNIIQRGGTMLRSARSEAFRTVEGRARAAANLKQAGIDALICI
                     GGGGTQSGAHQLVTEQGVRLIGIASTIDNDLGGSDRTIGFDTACNTALEAIDRLRDTA
                     SSYGRIFFVEVMGRDTGFIAMHTGIAAGAEYVMVPERREHVDGLVRVLENRSKGKGSS
                     IVVVAEGDQEGGAFKLAEVVKARCPQFDIRVSVLGHLQRGGNPTVTDRVMASRLGAAA
                     VDGLIRGEDHRVVGIVNGKVCFTPHAEAIAAKKTIEPDLFRLLPMLAI"
BASE COUNT          248 a          454 c          505 g          282 t
ORIGIN      
        1 atcgaggtag atctcgatgt cgttctcccc cgggttcaat tcctgtccgg cgatcgaatt
       61 ggccagggcc gttgcatacc aagtttgtgg tactggtgcg ccggtgaagt cctttcggcc
      121 gttcgggaag gtgatgccca gcacggagcc gttcatgggg atccaggtga ccatcgcctt
      181 gatgggcacg ggtgatacga ggatgccctc ccagatcgag gccgcatggt cgatggcgac
      241 ctgagcctcc tgtggggcga aattgtacgt gaccgaaatg gtcgcgcagt cggccgatgc
      301 gcaggagatg gcggtgccga tcagcaggag gggacgggac gggaacatgc gatggcggtc
      361 cgggtcagat ggcgagcatg ggaagcaggc ggaagaggtc aggctcgatc gtcttcttcg
      421 cggcgatcgc ctccgcatgc ggggtgaaac ataccttccc gttgacgatg ccgaccacgc
      481 gatgatcctc accgcggatg agcccgtcaa cggccgcggc gccgaggcgc gaggccatca
      541 cacggtcggt caccgtgggg ttgccgccgc gctgcaggtg gcccagcacg ctcacacgga
      601 tatcgaattg cgggcagcgc gccttcacca cttcggcgag tttgaacgca ccaccctcct
      661 ggtcgccctc ggccaccacc acgatggaac tgcccttccc tttggagcgg ttctcgagga
      721 cgcgcacaag cccgtccaca tgctctctgc gctcgggaac catgacgtat tccgcgccgg
      781 cagcgatacc ggtgtgcatg gcgatgaagc cggtatcgcg ccccatcact tccacgaaaa
      841 agatacgtcc gtagctgctg gcggtgtcgc gcaaccggtc gatcgcctcc agggcggtgt
      901 tgcaggccgt gtcgaagccg atcgtgcggt cgctgccgcc caggtcattg tcgatggtgc
      961 tggcgatacc gatcagtcga acaccctgtt ccgtgaccag ttgatgcgca ccgctttgtg
     1021 ttccgccgcc accgatgcag atgagggcat cgatcccggc ctgcttgagg ttggccgccg
     1081 ctctggcacg accttccacg gtcctgaacg cttcgctgcg tgcactgcgc agcatggtgc
     1141 cgccgcgctg gatgatgttc cgcacattcc tcggaccgag ttcgatgaaa tgggccttca
     1201 cgaggccgtc gaagccgtcc atcacgccgg agaccgggat gccgtggaag gccgcggtgc
     1261 gcaccactgc gcggatggcc gcattcatgc cgggggcatc gccaccggag gtgaggagtg
     1321 cgatccgggc aggcttcatg cgtgtatgct ttcagggggt gctgtgggag gtccgatccc
     1381 gatgtccgga tatggcgcgg agcgtacgcg gcttgacctg cctgacggtg acgtaccagt
     1441 tggggtcggt ggtcaacgtg gccgacgcgt gtacgttact ggccggtga
//
LOCUS       DAOQOC010000895         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_69992_length_1488_cov_2.877181, whole genome shotgun sequence.
ACCESSION   DAOQOC010000895 DAOQOC010000000
VERSION     DAOQOC010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69992_length_1488_cov_2.877181"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..1122)
                     /locus_tag="PL106_12885"
     CDS             complement(346..1122)
                     /locus_tag="PL106_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNA34013.1"
                     /translation="MKHIRTIITDDEPAAREHLVRLLAQDQELEVVAECRNGQEAVDA
                     VRKHRPDLLLLDIQMPQMNGLETVRAIPKDQLPFVVFVTAFDHYAIKAFELCAVDYLL
                     KPYDDERFFAALDKAKRHIALRENDRLSGRFMDIMREHLHARSEHVESFTIRDKGREH
                     RVQVADLVYLRAEGNYLWLQLKERHFLHRLTMNAVESELDPGRFLRIHRSYIVNLAHV
                     RSSRYSGNNEFIFTMANGERIVSGRSYKETIARALQEQTV"
     gene            complement(1128..>1488)
                     /locus_tag="PL106_12890"
     CDS             complement(1128..>1488)
                     /locus_tag="PL106_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA34014.1"
                     /translation="LHVSWEVPTALLGIQVPSMVLQPLVENSIKHGPDATTDRVEITV
                     RGERNGGHLTLRVEDNGKGCPDTERAMTNGGIGLRNVRERMKLLYGEHGALTIASPEG
                     RGFVASVSLPLVTELNN"
BASE COUNT          251 a          447 c          477 g          313 t
ORIGIN      
        1 catcaggaaa gtgtatgatc cgtgttcttc cgatgtggat gccgcagcag ttcatgggtt
       61 ccaagctcgt gtccgcggtc cgcgtggtcg ggtacgaccc gtgccatacc cgatcggacc
      121 gggatccgtc gatgcgcgat cgacgatggg acgtcgccct gagcatgcct cagggcacgt
      181 cagttgcgga aacgaccttg ccggtacccc accggacgaa aaacccgtgt tgcgccttga
      241 tccgcaccgg gtaggcgcat gccaatgccc cggcgaggag aagggcgact gttcggcata
      301 ccgcggacgg tcgtttgttc gatcccttct tcatggtctg tattttcaaa cggtctgttc
      361 ctgaagtgcg cgtgcgatcg tttccttgta gctgcgtccg ctaacgatgc gctcgccgtt
      421 ggccatggtg aagatgaact cgttgttgcc gctgtaccgg ctgctgcgca cgtgcgccag
      481 gttcacgatg taggaacgat ggatccgcag gaaccggccg ggatcaagct cactctctac
      541 cgcgttcatg gtaaggcggt gcaggaagtg acgctccttc aactggagcc agaggtaatt
      601 gccttcggcg cgcaagtaca cgaggtcggc cacctgcacc cggtgttcac ggcccttgtc
      661 gcggatggtg aagctctcca catgttcgct gcgggcatgc aggtgctcgc gcatgatgtc
      721 catgaaacgg cccgagagac ggtcgttctc gcgcagggcg atgtggcgtt tggccttgtc
      781 gagtgcggcg aagaaacgct cgtcgtcgta cggcttcagc aggtagtcca ccgcgcagag
      841 ctcgaacgcc ttgatggcgt aatgatcgaa agcggtgacg aagaccacga acggcaattg
      901 gtccttgggt atggcacgca cggtctccag cccgttcatc tggggcatct ggatatccag
      961 gaggaggaga tcgggccgat gcttccggac cgcgtccacg gcctcctggc cgttgcggca
     1021 ctcggccacc acttccagtt cctggtcctg tgcgagcagc cgcacgaggt gctcgcgcgc
     1081 ggcgggctcg tcgtcggtga tgatggtgcg gatatgtttc atcgggatca attgttcagt
     1141 tcggttacca gtggcagcga cacggaggct acgaagccgc gtccctcggg tgatgcgatg
     1201 gtgagtgcgc cgtgttcacc gtagagcagt ttcatccgtt cccgcacatt gcgcaatccg
     1261 atgccgccgt tggtcatggc ccgctccgta tcggggcaac ccttgccgtt gtcctccaca
     1321 cgcagcgtga gatggccgcc gttgcgttcg ccgcgcacgg tgatctccac acggtcggtg
     1381 gtggcatcgg gcccgtgctt gatgctgttc tccaccaacg gctgcaggac catgctgggc
     1441 acctggatcc ccagcagcgc ggtgggcacc tcccagctca catgcaag
//
LOCUS       DAOQOC010000896         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70258_length_1485_cov_3.743357, whole genome shotgun sequence.
ACCESSION   DAOQOC010000896 DAOQOC010000000
VERSION     DAOQOC010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70258_length_1485_cov_3.743357"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1485)
                     /locus_tag="PL106_12895"
     CDS             complement(<1..>1485)
                     /locus_tag="PL106_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNA34015.1"
                     /translation="ADKPTYGVGDNCVLTIPSGGTGRALVSLESGSRVLETRWVDLKE
                     KETRFEFPVTAEMAPNVYAHVMLVQPHERTRDNDRPMRLYGVIPIPVEDKATHVTPVI
                     AMAQEVRTDVPFNVEVAETDGKAMTYTLAIVDEGLLDLTRFKTPDPWDHFYAREALGV
                     RTWDLYDMVIGAFGQQMAHVLALGGSDDVAPDAAAKANRFKPVVRYVGPFSLAKGKKA
                     HHSFTISNYVGSVRVMVVATDGQRAYGNAEKTVPVRKPLMLLATLPRVAGPGETIDLP
                     VTVFAMDPKVKNVTLDLVTNDLFTVENGTQRQLSFNSMGDQVSVFRVRTKDRIGKGTV
                     KVTATGAGDQAVHHIELDVRQPNQPQTDMREAVVDAGRSWEDIPAPNGLPGTNSAYLE
                     VSTIPALDLGRRLQYLVSYPHGCIEQTTSAAFPQLFLADVIVTDSRTAGEMRGNVEAG
                     LRRLRSFQRPGGGFSYWPGGDDVNDWSTIWAGHFLVEAERAGFV"
BASE COUNT          238 a          501 c          455 g          291 t
ORIGIN      
        1 atacgaagcc cgcccgttcc gcctcgacca ggaagtgccc ggcccagatc gtgctccagt
       61 cgttcacatc gtccccgccg ggccagtagc tgaatccgcc gcccggtcgc tggaacgacc
      121 tgagtcggcg cagcccggcc tccacgttcc cgcgcatctc cccggctgtg cggctatcgg
      181 ttacgatcac gtcggcgagg aacaattgcg ggaaggccgc actggtggtc tgttcgatgc
      241 agccatgcgg gtagctcacc aggtactgga gcctgcgccc gaggtccagt gctggaatgg
      301 tgctcacttc caggtaggcc gagttcgttc cggggagccc gttaggtgcc gggatgtcct
      361 cccacgacct gccggcatcc accacggcct cacgcatgtc ggtctggggc tggttgggtt
      421 gtcgaacgtc caactcgata tggtggacgg cctgatcgcc ggcccccgtg gccgtcactt
      481 tgaccgtgcc cttgccgatg cggtccttgg tgcgcacccg gaacacgctc acctggtcgc
      541 ccatgctgtt gaagctgagc tgccgttgcg tcccgttctc cacggtgaag aggtcgttcg
      601 tcaccagatc caacgtcacg ttcttcacct tggggtccat ggcgaagacg gtgacgggca
      661 ggtcgatcgt ttcgcccggg ccagccacgc gtggcagggt ggccagcaac atcaacggct
      721 tgcgtaccgg gacggtcttc tccgcattcc cgtatgcccg ctggccatcg gtggccacca
      781 ccatcacccg cacgctcccc acatagttgc tgatggtgaa gctgtggtgg gctttcttgc
      841 ctttggccag cgagaagggg cccacgtagc gcaccacggg cttgaagcgg ttggctttgg
      901 ccgccgcgtc gggtgcgaca tcatcgctgc cgccgagcgc cagaacatgc gccatctgct
      961 gcccgaaggc accgatcacc atatcataaa gatcccatgt ccgcaccccg agcgcttcac
     1021 gcgcgtagaa atgatcccac gggtcgggag ttttaaaccg ggtcaggtcc agcaggccct
     1081 cgtccacgat cgcaagggtg tacgtcatgg ccttcccgtc ggtctcagcc acctccacgt
     1141 tgaacggtac atcggtgcgc acctcctggg ccatggcgat caccggcgtc acatgcgtgg
     1201 ccttgtcctc cacgggaatg gggatcacac cataaaggcg catgggccgg tcgttgtcgc
     1261 gtgtccgttc atgcggttgc acgagcatca catgggcgta cacgttgggc gccatctccg
     1321 ccgtaacggg gaactcgaac cgtgtttcct tctccttcag gtccacccac cgggtctcca
     1381 ggacacggct gccggactca aggctcacca gtgcgcggcc ggtccccccg gaagggatgg
     1441 tgagcacgca gttatcgccc accccgtatg ttggcttgtc ggcgt
//
LOCUS       DAOQOC010000897         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70295_length_1485_cov_2.751748, whole genome shotgun sequence.
ACCESSION   DAOQOC010000897 DAOQOC010000000
VERSION     DAOQOC010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70295_length_1485_cov_2.751748"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PL106_12900"
     CDS             complement(<1..323)
                     /locus_tag="PL106_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34016.1"
                     /translation="MNAMLPPRTFLLILTFCAACGGGNEVDISTIKNESAHEVAEQVE
                     HEAEQANEALDSLPSEVADSIRTEQKERQRIVQEMLKRSPYAAMSDAALDSMRNAWFR
                     AYVRS"
     gene            complement(320..>1485)
                     /locus_tag="PL106_12905"
     CDS             complement(320..>1485)
                     /locus_tag="PL106_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34017.1"
                     /translation="NLVADLSGRLDAPETYTDGVIIGLPLLDDLGLDTADLRGRKLLV
                     STGLFTFPLRVVAITRTLPERAKLCCDHALLRALEDHGTEGSIATDDVDEVTVLLSTD
                     EKELDANAGKWEQQLRGIVREARDIRIASASDMATHRVKAVITLERALTFEDGPGYFQ
                     EKLNEAEGDLAALEPRILLRTRFKGPDPPGAAPEDRPGREPFTDLTVTFRGDRLDGID
                     AFQRDIFQQQQVELDLDRIESKKNFAVVTKLGASLIGALVLFAALAITLFLYNTLKNH
                     LERIQMNIGTFLAFGIEERFLRNGYTRIVLELVLRTAALAMGTLLVMQGVLALMRGTG
                     AGTSGLLAHVDVLGNAWPYLALLLILATCFLIARAQLRRFLSRPPGDLIYART"
BASE COUNT          266 a          459 c          471 g          289 t
ORIGIN      
        1 catgaacgga cgtaagcccg gaaccaggcg ttccgcatgg agtcgagtgc cgcatcgctc
       61 attgcggcgt acggggatct tttgagcatc tcctggacga tccgttgccg ttccttctgc
      121 tcggtgcgta tgctgtcggc cacttcggag gggaggctgt caagggcctc attggcctgc
      181 tcggcctcgt gttccacttg ttcggccacc tcatgcgccg attcgttctt gatcgtgctg
      241 atgtccacct cgttgccacc accacaggct gcgcagaagg tgaggatcaa gaggaatgtc
      301 cgtggaggaa gcatggcatt catgtgcgtg catagatgag gtcccctggc gggcgggaca
      361 ggaaccgccg caattgtgcg cgggcgatca ggaagcaggt ggccaggatc aacaacaagg
      421 cgaggtaggg ccacgcgttg cccaacacgt ccacatgggc gagcagaccg gaagttccag
      481 cgccggtgcc gcgcatgagc gccagcacgc cttgcatcac cagcagcgtg cccatggcca
      541 gcgcggccgt gcgcaagacc agttccagta cgatccgggt gtacccgttc cggaggaagc
      601 gttcttcgat accgaaggcc aggaaggtgc cgatgttcat ctggatgcgt tccaggtggt
      661 tcttcaacgt gttgtaaagg aacagcgtga tggccagtgc ggcgaacagg accagggcac
      721 cgatgagcga tgcccccagt ttggtcacca ccgcgaagtt ctttttcgat tcgatgcgat
      781 ccaggtccag ttccacctgc tgctgctgga agatgtcgcg ctggaacgcg tcgatgccgt
      841 ccaaccggtc accacggaag gtgaccgtga ggtcggtgaa cggctcgcgt cccggccggt
      901 cctcgggcgc ggcaccgggg ggatcgggtc ccttgaagcg cgtgcgcagc aggatacgtg
      961 gctccagtgc cgccagatcg ccttcggcct cgttcaactt ctcctggaag tagccgggcc
     1021 catcctcgaa ggtgagcgca cgctccaagg tgatcacggc cttcacgcgg tgggtggcca
     1081 tgtccgatgc cgacgcgata cgtatatccc tggcctctcg tactattccg cgtagttgtt
     1141 gttcccattt ccccgcgttc gcatccaact ctttctcatc ggtgctcaac agcacggtca
     1201 cctcgtccac atcgtccgtg gcgatcgatc cttcggtccc gtgatcctcc aaggcccgca
     1261 gcagggcatg gtcacaacag agcttggccc gctcgggcag ggtccgcgtt atggcgacaa
     1321 cgcgcaaggg gaaggtgaac aggccggtgc tcaccagcag cttccgcccg cgcagatcgg
     1381 cggtatcgag cccgaggtcg tccagcaggg gcaggccgat gatcacacca tcggtgtatg
     1441 tttcaggcgc gtccagacgc ccactgaggt cggccaccag gttgc
//
LOCUS       DAOQOC010000898         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70350_length_1484_cov_3.825052, whole genome shotgun sequence.
ACCESSION   DAOQOC010000898 DAOQOC010000000
VERSION     DAOQOC010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70350_length_1484_cov_3.825052"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            474..938
                     /locus_tag="PL106_12910"
     CDS             474..938
                     /locus_tag="PL106_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34018.1"
                     /translation="MNTVKVKICCISSLEEAQLATAHGAYALGLVGHMPSGPGVIDDA
                     LITDIARAVAPPIRTFLLTSETSAEAVIAHHRRVRTTTVQIVDALEERQYDLLRRELP
                     GVELVQVIHVLDEASVREAVEVSQHVDAILLDSGNPNLAVKELGGTGRAHDW"
     gene            939..1124
                     /locus_tag="PL106_12915"
     CDS             939..1124
                     /locus_tag="PL106_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34019.1"
                     /translation="MSKAIREAINVPLFLAGGLNAGNVQRAVDTVQPFGVDLCSSVRT
                     DGRLDERKLASFFSALS"
     gene            complement(1147..>1484)
                     /locus_tag="PL106_12920"
     CDS             complement(1147..>1484)
                     /locus_tag="PL106_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HNA34020.1"
                     /translation="RTREGHGGNNPEDNLFSQINTKNGRHVGHIGIQYTLPMLFVLDT
                     RIDTDGKLRAQLMREDLPLTPHLRLDLMANTDLEYMAALRYVIDKSWAVRTHYDSDMG
                     FGVGVAVTY"
BASE COUNT          286 a          435 c          481 g          282 t
ORIGIN      
        1 gaactgctcc agagcgcgac cgcgatcaac gccaagctgc tcgcgcagcc caacacgatc
       61 atgcgtttgc ggcgggcagg gcgaggggcg atcgcgcggt ccatgtacgt gtggatccca
      121 aatttggatc aacccgtccc cctcaagagg atgaatgtgc ggggtggaag aaggtgtgac
      181 gaatggtgtg cttcatacgt tagcagcagc accagcccat gtcgctgtcg tagtgcgaga
      241 aggccgcctt gccctctgtc gccttgcacg tactcagtag gtggttaata tacggtttga
      301 ccggcgatca accatggtgt gtcgaagggt gcaagagtgc gaggctcgaa cccttgcact
      361 ctcgcacttt tgaacccatg gatcgaccgc ttccttatca ccgtctgagt agcacccgtt
      421 gaagaacgag tgtgctacct ttcacgaacg atcatccatt gaaccacgag cgcatgaaca
      481 cggtgaaggt gaagatctgc tgcatcagca gcttggaaga ggcgcaactg gcgaccgcgc
      541 acggtgccta tgccctggga ctggtcggcc acatgccaag cggacccgga gtgatcgacg
      601 atgccttgat caccgacatc gcacgtgccg tggcaccgcc catacgcacc ttcctgctca
      661 cgagcgagac cagtgcggaa gccgttatcg cgcaccatcg gcgggtgcgc accaccaccg
      721 tacagatcgt ggatgcgttg gaagagcggc aatacgacct tctgcgccgc gagctgcccg
      781 gcgtggaact ggtacaggtg atccatgtgc tggacgaggc ctccgtgcgc gaagcggtgg
      841 aagtttccca acacgtggat gcgatcctgc tggacagcgg caacccgaac ctggcggtga
      901 aggagctcgg cggcacgggc cgagcgcacg actggtagtt gagcaaagcc atccgcgaag
      961 ccatcaacgt tccgctcttc ctcgccggcg gactgaacgc gggaaatgtg cagcgcgccg
     1021 tggacacggt gcagcccttt ggtgtggacc tgtgcagcag cgtgcggacc gatggaaggt
     1081 tggatgagcg gaagctcgcg tcgttcttca gcgcgctgtc gtagtgctgg agaagcaggg
     1141 ttgccttcaa tacgtcaccg ccacgcccac cccaaagccc atgtcgctgt cgtagtgcgt
     1201 acgcacggcc cacgacttgt cgatcacgta gcgcagggcg gccatgtatt ccaggtcggt
     1261 gttggccatc aggtccaggc ggaggtgcgg ggtgagcggc aggtcctcgc gcatgagctg
     1321 ggcgcgcagc ttgccgtcgg tatcaattcg ggtgtccagc acgaagagca tgggcagcgt
     1381 gtactggatg ccgatgtggc ccacatgccg cccgttcttg gtgttgatct gggagaagag
     1441 gttgtcctcc gggttgtttc caccgtggcc ttcgcgcgtg cgcc
//
LOCUS       DAOQOC010000899         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70514_length_1483_cov_2.243697, whole genome shotgun sequence.
ACCESSION   DAOQOC010000899 DAOQOC010000000
VERSION     DAOQOC010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70514_length_1483_cov_2.243697"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..489
                     /locus_tag="PL106_12925"
     CDS             64..489
                     /locus_tag="PL106_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNA34021.1"
                     /translation="MATRSFEDALAWQRAMDLNVAIDKALAESKNWGFKDQLFRTALS
                     ICNNLAEGYEMPTTPHQLKYLWIAKGSCNEVCSMLILARRRGYFPDEVIDHMMGLQDE
                     IGRLIRTYINHKSKGWGNIPGAMVFLGLWFNLTTPWFLR"
     gene            complement(566..>1483)
                     /gene="metK"
                     /locus_tag="PL106_12930"
     CDS             complement(566..>1483)
                     /gene="metK"
                     /locus_tag="PL106_12930"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNA34022.1"
                     /translation="GAGDQGMMFGYACRDTDNYMPLPLELSHLLLRELAVIRREKNSP
                     MPYLRPDAKSQVTIEYNDDHTPKRTDAIVVSTQHDDFDKEAVMLAKIKKDVIGILIPR
                     VMKKLPKRVQALFGKDIAYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGA
                     FSGKDPSKVDRSAAYAARHVAKHLVAAGVCDEVLVQVAYAIGVAKPVGFYVNTYGTAK
                     VNMTDGQIAKKIGALPEFDMRPYFIEKRFALRTPIYSETASYGHMGRESKVVAKSFNN
                     AGQKTTVKVKLFPWEELNAVGAVKKAFEL"
BASE COUNT          279 a          433 c          438 g          333 t
ORIGIN      
        1 gtggggtcga taggtctgga gggtccgaga catttaacat tgtcatgacc aacaaacaac
       61 ctgatggcta cccgcagttt tgaggacgcg ctcgcatggc agcgcgctat ggatttgaac
      121 gttgcgattg acaaggcact tgcggagagc aagaattggg gtttcaagga ccagctattt
      181 cgtactgcac tttccatttg caacaacctg gcggagggtt atgaaatgcc gaccaccccg
      241 caccagctga agtacttgtg gatcgccaag ggttcgtgca acgaagtgtg ctcaatgttg
      301 atccttgcga gacgcagagg atacttcccc gatgaggtga tcgatcacat gatgggattg
      361 caagatgaga tcggacgcct gatccgcacg tatatcaacc acaagtcgaa aggctggggg
      421 aatatacccg gagctatggt gttcttgggg ctatggttca acctgaccac accttggttc
      481 ctccggtagg cgagagacaa gggacgagag acgaggctgc ctgatggaag cagcctcgtc
      541 tctcgtctca gagcactcgt ctctcctaca gctcgaacgc cttcttcaca gcgcccaccg
      601 cattcagttc ctcccagggg aagagcttca ccttcacggt agttttctgc ccggcattgt
      661 tgaaggactt ggcgaccact ttgctctcgc gccccatgtg gccgtagctg gcggtctcgc
      721 tgtagatcgg tgtgcgcagg gcgaagcgct tctcgatgaa gtaggggcgc atgtcgaact
      781 ccggcagtgc gccgatcttc ttcgcgatct ggccgtcggt catgttgacc ttggcggtgc
      841 cgtacgtgtt cacgtagaaa ccgaccggct tcgccacgcc gatggcgtac gccacctgca
      901 caagtacttc atcgcacacc cctgcggcca ccaggtgctt ggccacgtga cgtgccgcgt
      961 acgccgcgct gcgatccacc ttgctcgggt ccttgccact gaatgcacca ccaccgtgcg
     1021 cgcctttgcc accgtacgtg tccacgatga tcttgcgacc cgtgaggccg gtatcgccgt
     1081 gcggaccgcc gatcacgaat ttgcccgtgg ggttgatgtg ataagcgatg tccttgccga
     1141 aaagcgcttg tacacgcttg ggcagtttct tcatcacgcg cgggatcagg atgccgatga
     1201 cgtccttctt gatcttcgcg agcatcaccg cttccttgtc gaagtcgtcg tgctgggtgc
     1261 tcaccacgat cgcgtcggtg cgcttgggcg tgtggtcgtc gttgtactcg atggtcacct
     1321 ggctcttcgc atccgggcgc aggtacggca tcgggctgtt cttctcgcgg cggatcacgg
     1381 cgagttcacg cagcagcaga tggctcaact ccagcggcaa cggcatgtag ttgtccgtat
     1441 cgcggcaggc gtaaccgaac atcatgccct ggtcgccggc gcc
//
LOCUS       DAOQOC010000900         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70567_length_1482_cov_3.138052, whole genome shotgun sequence.
ACCESSION   DAOQOC010000900 DAOQOC010000000
VERSION     DAOQOC010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70567_length_1482_cov_3.138052"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PL106_12935"
     CDS             complement(<1..1166)
                     /locus_tag="PL106_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34023.1"
                     /translation="MRIAEVTDARTLRDWTKLPWRIYVNDPNWIPHLKQDVEKVFDPE
                     KNKLLGELEGHRGAISRWVLYADDGSTIGRIAAFVNPKTAFTEKDQPTGGCGFFECID
                     DQRAADLLFNTARDWLKARGMEAMDGPVNLGDRNMFWGLQIMNFTDPPLYGTNYNPAY
                     YIGLFERYGFGLWFKQHFFKRSMSVPAQPIFYRKYKQLAEDPRFRMHDGRGRTVQQLA
                     RDFCDVYNAAWVDHENHKAMEPSAALKIVKAMKPVMDPRLLIFIRYDDKAVAMYISLP
                     ELNEIFKHVNGDLNWIGKLKFLWHKWRGTVQNMTGMVFGVAKEWQGKGLEGAMIVYAD
                     KHLVSNKLYKDTVLTWIGDFNPRMLKVCQNLGTENYRDLATYRYLFDRTRTFER"
BASE COUNT          249 a          462 c          438 g          333 t
ORIGIN      
        1 atccgctcaa aggtgcgcgt ccggtcgaaa agataccggt aggtggccag gtcgcggtag
       61 ttctccgtgc cgaggttctg gcacaccttc agcatgcgcg ggttgaaatc gccgatccag
      121 gtgagcaccg tgtccttgta cagtttgttg ctcaccagat gcttgtcggc gtacacgatc
      181 atggcccctt ccaggccctt gccctgccat tccttggcca cgccgaacac catgccggtc
      241 atgttctgca cggtgccgcg ccatttgtgc cacaggaatt tcagcttccc gatccagttg
      301 aggtcgccgt tcacatgctt gaagatctcg ttcaactccg gcaggctgat gtacatggcc
      361 acggccttgt cgtcgtaccg gatgaagatg agcaggcgcg ggtccatcac cggcttcatg
      421 gccttcacga tcttcagggc cgcggacggt tccatggcct tgtggttctc gtggtccacc
      481 caggccgcgt tgtacacgtc gcagaagtcc ctggccagtt gctgcaccgt gcgcccacgg
      541 ccatcgtgca tcctgaatcg cgggtcctcg gccagttgct tgtacttgcg gtagaagatc
      601 ggttgggccg gtaccgacat gctccgcttg aagaaatgct gtttgaacca gagcccgaag
      661 ccgtaccgct cgaagagccc gatgtagtac gccgggttgt aattggtacc gtacagcggt
      721 ggatccgtga agttcatgat ctgcaacccc cagaacatgt tccggtcgcc caggttcacg
      781 ggcccgtcca tggcctccat gccccgtgct ttcagccagt cgcgcgccgt gttgaacagc
      841 aggtccgccg ctcgctggtc gtcgatgcat tcgaaaaaac cgcagccgcc ggtgggctga
      901 tccttctccg tgaacgcggt cttcgggttc acgaatgcgg cgatgcggcc gatggtgctg
      961 ccgtcatcgg cgtacagcac ccaccgggag atcgcgcccc gatgcccctc gagttcgccc
     1021 agtagtttgt tcttttccgg gtcgaagacc ttctccacat cctgtttcag gtgagggatc
     1081 caattggggt cgttcacgta gatgcgccaa ggcagtttcg tccagtcgcg caacgtacgg
     1141 gcgtcggtca cttcggcgat cctcatgggg cgaagatggc tcttcctttt tttgttaccg
     1201 cacggcaggc agaacgcggc agccagcggg ccgtaagcgg ctggccgcac attccacatg
     1261 aggggtgccc tgatgagcgg ccgctgcccg tcgtctgctg cacttttcaa ttcccttgct
     1321 ttgccaacgg aatatcacga ccccggatca tgcccgtctt ccttgtcggt ttcatgtgca
     1381 gtggaaaaag ccgtatcggc cgcgaactgg ccgggcgcat gggctggcgc ttcctggata
     1441 tcgatcggga gatcgagcgg cgcatcggcc ccatcacgcc tt
//
LOCUS       DAOQOC010000901         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70733_length_1481_cov_1.746844, whole genome shotgun sequence.
ACCESSION   DAOQOC010000901 DAOQOC010000000
VERSION     DAOQOC010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70733_length_1481_cov_1.746844"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="PL106_12940"
     CDS             complement(<1..1465)
                     /locus_tag="PL106_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34024.1"
                     /translation="MWGGFVLYRNSAEGFRTIDLTVAGYPAPRRGPTPTRNDGHCLLE
                     PVRLDDDGQLLCWCRTTNEFDLVHLRLNGDRAEVLGRYSAVAGAPLRGLDLRSTPGTP
                     GTYVLQRTPDGVQVLLVDREGHATSLGRHAALPSDMKWGSLAMPITERTWLLGTAVRS
                     WSPGTDPLLADTLFDLAARYPEASFMVNDALRDRLGHIWLATDFGLFKLTLQPDRFQR
                     LLHEPQLKQAFGKRIRGLVVQHERVYVNTDMEGYFVLDARTGAVLRSDTARVVRLALV
                     PDGHGGVWRAQARTIVHEDADGRVLRTVNSAGRLVWSLVSAPDGSVFAGSDRGLFRVA
                     QADSVDRIIHAQHSMFDDARIVHLSRAHNGRILACTDAGLFELDANGEVLDRWWTGAD
                     RAKDAAHVLPTDDIRHVYVDSAGLHWLATGTRGLLRWDRRTGELREIGPREGLPSASI
                     HAVYPDEAGWLWLPSNGGLVRYRPSNGQVTVITTANGI"
BASE COUNT          258 a          507 c          482 g          234 t
ORIGIN      
        1 cgatgccgtt cgctgtggtg attaccgtga cctgtccgtt gctcggccgg tagcgcacca
       61 ggccaccgtt gctcggcagc cagagccagc cggcctcatc cggatacacc gcgtggatgc
      121 tcgcggaggg aagtccttcg cgcggaccga tctcgcgcag ttcgcccgtg cggcggtccc
      181 aacgcagcag cccgcgcgtg cctgtggcca gccaatgcag gccggcgctg tccacgtaca
      241 cgtggcggat gtcgtccgtg gggagcacgt gcgcggcatc cttcgcgcgg tcggcaccgg
      301 tccaccagcg gtccagcact tcgccgttcg cgtcaagctc aaagagcccg gcatcggtgc
      361 aggcgaggat ccgtccgttg tgcgcacgcg atagatgaac gatgcgcgcg tcgtcgaaca
      421 tgctgtgttg cgcatggatg atgcgatcga cactgtccgc ctgcgccaca cggaacaggc
      481 cgcgatcact tccggcgaac acgctgccat cgggtgccga caccaagctc cacaccaacc
      541 gcccggcgct gttcaccgtg cggagcacac gtccgtccgc atcctcatgc acgatcgtgc
      601 gcgcctgtgc gcgccacaca ccgccgtgtc catccggcac caacgccaag cgcaccacgc
      661 gcgcggtgtc gctgcgcagc acggcaccgg tgcgggcgtc cagcacgaag tagccttcca
      721 tgtccgtgtt cacgtacacg cgttcgtgct gcaccaccag accgcgtata cgtttaccga
      781 aggcctgttt caactgcggc tcatgcaaca gccgctggaa gcggtccggt tgcagggtga
      841 gcttgaagag cccgaaatcc gtggccagcc agatgtggcc gagacggtcg cgcaaagcgt
      901 cgttcaccat gaacgaggct tccgggtacc gcgcagcgag gtcaaagagc gtatcagcca
      961 gcaacggatc ggtgccgggc gaccagctgc gcacggcggt gcccagcaac caggtacgct
     1021 ccgtgatcgg catggccaaa ctgccccact tcatgtcgga aggcaaagcg gcatgccggc
     1081 ccaaactggt cgcatggcct tcgcggtcca ccaacagcac ctgcacgccg tccggtgtgc
     1141 gttgcaatac gtaggtgccc ggtgtaccgg gcgtggaacg aagatccagt ccgcgaagcg
     1201 gagcgccggc caccgcgctg tagcggccga gcacctcggc acggtctccg ttcaaccgca
     1261 ggtgcaccag atcgaactcg ttggtggtgc ggcaccagca caagagctgt ccatcgtcgt
     1321 ccaggcgcac gggttccagc aggcaatggc catcgttgcg cgtgggcgtg ggtccgcggc
     1381 gcggtgcggg atagccggcc accgtgaggt cgatggtgcg gaagccttcc gcgctgttgc
     1441 ggtacagcac gaagccgccc cacacgccga gcgcgatggt g
//
LOCUS       DAOQOC010000902         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_70799_length_1480_cov_2.610526, whole genome shotgun sequence.
ACCESSION   DAOQOC010000902 DAOQOC010000000
VERSION     DAOQOC010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70799_length_1480_cov_2.610526"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1480)
                     /locus_tag="PL106_12945"
     CDS             complement(<1..>1480)
                     /locus_tag="PL106_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34025.1"
                     /translation="GTGVLCNDLCTGAETVTCGSVVSATTVGSTIDGTPSQTSGGSTT
                     VQYGVWYKYIGDDQQVTVSTCGGPIGDTRLHVYRGSCGTLIGVAGNDDAGASCSTYAL
                     QSTVTFNAFIGQTYYIAVERYSGTGTFQLTTTCGPLCTPVVANDACVDAQTVLMQANC
                     DPVGGNLSCATTTAGANPSCAGSSFASFPDAYYTFVATGPDAYINLTFSNPGLKFVLY
                     NGGCGSTQMQCSSPVSGVPTLVTGLVPGNTYTLRVFQLLADAGTFSLCIQKLDVSDDP
                     CTPVALTCGDTRFGRTVGRLNNIPTGACPFNGAASTGGVNFFSYTAAEDGDVTFSTCG
                     STDFNTRLSVFTGACGNLACTVMNDDAAGCPNNSSTTTLRATTGQTYLIMVHGSGAAE
                     GNYQIGVFCEPWCAPSEGNDRCAAASPVTIWHQDDPLALPSTEWLACSYHDAPTSCSA
                     TGAVQGVWFTFTTTASTTDYTLYLLDNSDNPAYTAPTLSATLY"
BASE COUNT          267 a          436 c          489 g          288 t
ORIGIN      
        1 atacagggtt gcgctgagcg tgggagcggt gtaggccggg ttgtcgctgt tgtccaagag
       61 atacagggtg tagtcggtgg tgctcgccgt ggtggtgaag gtgaaccaca cgccttgcac
      121 cgctccggtc gccgagcaac tcgttggtgc atcgtgatag gaacaggcca gccactctgt
      181 tgaaggaagg gcgagcggat cgtcctgatg ccatatcgtt acgggagatg ctgctgcaca
      241 gcggtcgttc ccttccgacg gagcgcacca gggctcgcag aacacgccaa tctggtagtt
      301 gccctcggcg gcacccgagc cgtgcaccat gatgaggtag gtctggcccg tggtggcccg
      361 caatgtggtg gtgctggaat tgttcgggca tccggccgca tcatcgttca tcaccgtgca
      421 ggcgaggttc ccgcaagcac ccgtgaatac cgataggcgt gtattgaagt ccgtggatcc
      481 gcaggtactg aaggtcacgt cgccatcctc ggccgcggtg tacgagaaga agttcacacc
      541 gccggtggag gccgctccgt tgaacggaca ggcgcccgta gggatgttgt tcaatcgtcc
      601 cacggtacgt ccgaaacggg tgtccccgca ggtcaatgcc accggtgtgc aggggtcgtc
      661 gctcacatcc agcttctgta tgcacaggct gaacgtgccg gcatcggcca gcagttggaa
      721 cacgcgcagg gtgtaggtgt tgcccggcac gaggcccgta accagcgtag gcacgcccga
      781 gacagggctg gagcactgca tctgcgtgct gccgcaccca ccgttgtaca gtacgaattt
      841 gagtccggga ttgctgaagg tgaggttgat gtacgcgtca ggcccggtgg ccacgaaggt
      901 gtagtacgca tcggggaagc tggcgaaact gcttcccgcg caactcgggt tggcacccgc
      961 cgttgtcgtg gcgcaactga ggttgccgcc caccggatcg cagttggcct gcatcaggac
     1021 cgtttgcgca tccacacagg cgtcattggc caccaccggg gtacacaacg gcccgcaggt
     1081 cgtggtgagt tggaaggtcc cggttcccga gtatcgttcc accgcgatgt agtacgtctg
     1141 accgatgaag gcattgaagg tcaccgtgga ttgcagggcg taggtggagc aactcgcgcc
     1201 cgcatcatcg ttgccggcca cgccgatcaa tgttccgcag gagccgcggt acacgtggag
     1261 ccgcgtgtcc ccgatcggac caccacacgt gctcaccgtc acttgttggt catccccgat
     1321 gtacttgtac catacgccgt actggacggt ggtgctaccg ccggaggtct ggctcggcgt
     1381 gccatcaatg gtggatccaa cggtggtggc actgaccaca ctgccacagg tgaccgtctc
     1441 ggccccggtg cataggtcgt tgcacagcac gcctgtgcct
//
LOCUS       DAOQOC010000903         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71023_length_1478_cov_2.146873, whole genome shotgun sequence.
ACCESSION   DAOQOC010000903 DAOQOC010000000
VERSION     DAOQOC010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71023_length_1478_cov_2.146873"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PL106_12950"
     CDS             <1..1185
                     /locus_tag="PL106_12950"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA34026.1"
                     /translation="VTAGLPSNTVRAIAFGPDGDTWVGTDWGLCRYDGSTWVLYQEGT
                     SGLPENDIRALAFDPQGRLWIGLFSQGLVVKDGEDWHQYMPGTSPMPSEQVHNIVFDP
                     VGNGWVCTTNGLARTDLDEWRIYNNTDTSYAGLMLPGVNIKDIVVREDGLACIGTLNA
                     GFVYLTDTAVLVYNSFQNQLPDNTALGVAVDSDGDRWTACPYGGLVRFSGPYNNGLFF
                     QFVTEFTGIPSNSLSDIVIDAADRKIIATQNAGLTILAADNNTWTTYNTGNSGLPDND
                     LICVELAPDGAIWTGTSEGGVARFDPVAAMDDDRLLPTPQVFPNPFDQDVTVHWSVHD
                     RMFWSLNDASGRSLDQGFHLTGDPFHLSLGHLQPGVYTLSLTAGGGNSSMRLIRVPVG
                     RN"
     gene            1269..>1478
                     /locus_tag="PL106_12955"
     CDS             1269..>1478
                     /locus_tag="PL106_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34027.1"
                     /translation="MDRSSAWRILGICTGLAVAPLLNGQCTTSNGTGCQCEEAGQTNC
                     DLLPDITISWYALQNYSGGPNEYAQN"
BASE COUNT          305 a          439 c          432 g          302 t
ORIGIN      
        1 gtcaccgccg ggctgcccag caacaccgtg cgtgccatcg ccttcggccc tgatggcgat
       61 acctgggtgg ggactgattg gggactatgc cgctatgacg gatccacgtg ggtgctctac
      121 caggagggca ccagcggctt gcccgagaac gatatccgtg cgctcgcctt cgacccgcaa
      181 ggaaggctgt ggatcgggct cttttcccaa ggactggtgg tgaaggacgg ggaggactgg
      241 caccagtaca tgcccggaac cagtcccatg ccatccgaac aggtacacaa catcgtattc
      301 gacccggtcg gcaacgggtg ggtctgcacc accaacgggc tggcccgtac cgacctggat
      361 gaatggcgca tctacaacaa caccgatacc agttatgcgg ggctcatgtt gcccggggtg
      421 aacatcaagg acattgttgt gcgtgaggac ggcctcgctt gcatcggtac gctcaatgcc
      481 ggcttcgtct acttgacaga taccgctgtt ctggtttaca actcattcca gaaccagttg
      541 ccggacaaca cggcactggg ggttgcggtc gatagcgatg gcgaccgatg gacagcgtgc
      601 ccatacgggg gcctcgtgcg gttctcaggg ccgtacaaca acgggctttt cttccagttc
      661 gtcaccgaat tcaccgggat cccgtcaaac tcgctcagcg atatcgtcat cgatgcggcc
      721 gaccgcaaga tcatcgctac ccagaatgcc ggactcacga tcctggctgc cgacaataac
      781 acatggacca cgtacaacac cggcaattcc ggccttccgg ataatgacct gatatgtgtg
      841 gaattggctc cggatggtgc tatttggaca gggaccagcg aaggaggcgt ggcgcgattc
      901 gatccggtgg ccgccatgga cgatgaccgg ttgctgccca cacctcaggt attcccgaac
      961 cccttcgacc aggatgtgac ggtccactgg tccgtacatg accggatgtt ctggagcttg
     1021 aacgatgctt cagggcgctc gctcgatcag gggtttcact tgactggcga tccgttccac
     1081 ctgtcacttg gtcacctcca gcccggggtg tacaccctga gcctaacggc aggggggggc
     1141 aacagctcaa tgcggctgat cagggtaccc gtgggacgga attgaggcat aacttgacca
     1201 ttgtcattgc ggtttctaaa tttggatcgc tcaactcggc aactcaactg accgggtaca
     1261 aacttcacat ggatcgatct tccgcatggc ggatccttgg gatctgcacg ggcttagctg
     1321 ttgctcccct tctgaacggg cagtgtacaa cctccaacgg taccggctgc cagtgcgagg
     1381 aagcgggcca gaccaattgc gatctgctgc ccgatatcac catcagctgg tacgctttgc
     1441 agaactattc cggcggcccc aacgagtatg cgcagaac
//
LOCUS       DAOQOC010000904         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71064_length_1477_cov_4.154712, whole genome shotgun sequence.
ACCESSION   DAOQOC010000904 DAOQOC010000000
VERSION     DAOQOC010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71064_length_1477_cov_4.154712"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PL106_12960"
     CDS             complement(<1..387)
                     /locus_tag="PL106_12960"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNA34028.1"
                     /translation="MNAFQSLLLAVLLTAFAPLEEKPDVPALYRACGLVGALNEEVFT
                     TALIKAEGFGPLPDVLAVVDMTQPSTAKRLFVVDLKHRKLLLRTWVAHGQGSGGDRCV
                     RTSNDDGSHCTSKGLLRVGQHVISPKH"
     gene            complement(425..>1477)
                     /locus_tag="PL106_12965"
     CDS             complement(425..>1477)
                     /locus_tag="PL106_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNA34029.1"
                     /translation="SDSVKLSHPEHIIVTDTLHYTTTTGVADFFGPTRIIQGATLMFC
                     ERGSYDTRADRGLFTRAGRILDGAQELRGDSLLFDGRAGEGRGWGHVTMIDTSSNLLV
                     RGQQGLHLQHADSSYITGRAELVMLMGEDSLFLHADTLSATTDSSRGQRVLARRNVRF
                     FKNDMQGVCDTMTWADRDSLITLAGSPYLWSGKDQISGRLITIALRNGHAHRLHVLRD
                     ALLANSVDSLPEDADPTAAYFDQVTGTTITGWFAHDKLVRIEAEGNSRTVSFAKEKDE
                     SGTERVTTMNRADCSRIVVRLDSGEVSSISFLVKPDAVMYPIGQAPVEEMRMKGFTWN
                     AAARPVDRISIFAPEE"
BASE COUNT          258 a          451 c          484 g          284 t
ORIGIN      
        1 gtgcttaggg ctgatgacat gctgaccgac ccgcaggaga cctttgctgg tacaatgcga
       61 gccatcgtcg ttgctggtgc gcacgcagcg atccccacca cttccctgcc cgtgcgccac
      121 ccaggtgcgc aaaagcagct tgcggtgctt caggtccact acgaacagcc gcttggcggt
      181 gctgggctgt gtcatgtcca caacggcgag cacatcaggc agggggccga agccctccgc
      241 cttgatcagt gcggtggtga agacctcttc gttcagcgcg ccaacaaggc cgcacgcgcg
      301 gtacaaggcg ggcacatcgg gcttctcctc caatggcgcg aatgccgtga ggagtacggc
      361 cagaaggagg gactggaaag cgttcatccg gtcggttcta cggaagaacg tgggaacggg
      421 tcgttcattc ttcgggggcg aagatgctga tcctgtcgac agggcgggcg gccgcgttcc
      481 aggtgaagcc cttcatgcgc atctcttcca ccggcgcctg gccgatgggg tacatcaccg
      541 catcgggttt caccaggaag gagatgctgc tgacctcacc gctatcgagt cgcaccacga
      601 tgcggctgca gtcggcccgg ttcatggtgg tcacgcgttc cgtgccgctc tcatccttct
      661 ccttggcgaa ggagacggtg cggctgttgc cctcggcctc gatacgcacc agcttgtcgt
      721 gggcgaacca accggtgatg gtggtaccgg tcacctggtc gaaatacgct gcggtggggt
      781 ccgcgtcctc cggcagactg tccacgctgt tggccagcaa ggcatcccgc agcacgtgca
      841 ggcgatgggc atggccgttg cgcagcgcga tggtgatgag gcgaccactg atctggtcct
      901 taccgctcca caggtagggt gagccggcca aagtgatcag gctgtcccga tcggcccatg
      961 tcatggtatc gcatacgccc tgcatgtcgt tcttgaaaaa gcgcacgttg cgtcgggcga
     1021 gcacccgttg gcccctggag ctgtcggtgg tcgcggagag cgtatcggca tggaggaaga
     1081 gggagtcctc ccccatcagc atcaccagtt cggcgcggcc ggtgatgtac gagctgtcgg
     1141 cgtgttggag atgcaggcct tgctggccgc ggacaagcag gttgctgctg gtgtcgatca
     1201 tggtcacatg gccccagccg cggccttccc ctgcccgtcc atcgaacagg aggctgtcgc
     1261 cccggagttc ctgcgcgccg tcgaggatgc ggcccgcacg cgtgaacagg ccgcgatcgg
     1321 cacgggtatc atagctgccc cgctcgcaga acatcagggt ggctccctgg atgatccgcg
     1381 tgggcccgaa gaaatcggcc accccggtcg tggtcgtata atgcagcgtg tccgtaacaa
     1441 taatatgttc aggatgggat aatttcacac tgtcgct
//
LOCUS       DAOQOC010000905         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71075_length_1477_cov_3.692686, whole genome shotgun sequence.
ACCESSION   DAOQOC010000905 DAOQOC010000000
VERSION     DAOQOC010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71075_length_1477_cov_3.692686"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PL106_12970"
     CDS             complement(<1..1371)
                     /locus_tag="PL106_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNA34030.1"
                     /translation="MSGLLTLQQIRDFKGTYPKQLFYLFGTEMWERFCFYGLRGMLVV
                     FMATELAMPEKQANLQYGAIQAFVYAFTFLGGVFADKILGFQKSLFWGALLMIVGGFV
                     IAAAPKELFYIGICFNIIGTGFFKPNISTMVGQLYNDGDHRRDAGFGLFYMGINIGAF
                     LGGLLMVWVGKSYSWRAAFALVGVVMIISLINFAFRRKTFGPIGLSPLHPDMPANKRK
                     LYEWGTYIGSLLVIPLILVMVTNTAYTDMFMYIVGPLTLVYLAWEMRDFSKAENMKLA
                     AALIFIFFSIFFWAFFEQSGGSLSLFALNNLHPSLLGITVDTNSVNNSANSLFVIAFS
                     ALAGLFWLWLSKRKAEPNSVVKFGFGFLFLAAGFYIFKSTLGFADANGITSTEVFTFA
                     WFVITFGELCLSPIGLSLMTKLSPVRIQGLMMGMWFLASAYGQYAAGLLGAGMSAENP
                     DATNYDK"
BASE COUNT          330 a          409 c          476 g          262 t
ORIGIN      
        1 cttgtcgtag ttggtagcgt cggggttttc ggcgctcatg cccgcgccca agagaccggc
       61 ggcgtattgg ccgtatgcgc tggccaagaa ccacatcccc atcatcaggc cctggatgcg
      121 cacgggggag agcttggtca tcagggagag gccgatgggc gagaggcaga gttccccgaa
      181 ggtgatcacg aaccaggcga aggtgaagac ctcggtgctg gtgatgccgt tcgcatcggc
      241 gaagcccaag gtgctcttga agatgtagaa gcccgcggcc aggaagagga aaccgaagcc
      301 gaacttcacc acgctgttgg gctcggcctt gcgcttgctg agccacaacc agaacaggcc
      361 agccagggcg ctgaaggcga tcacgaacag tgagttggcc gagttgttca cactgttggt
      421 gtccaccgtg ataccgagga gcgaggggtg caggttgttc agggcgaaga ggctcaggct
      481 gcccccgctc tgctcgaaga aggcccagaa aaagatgctg aagaagataa agatgagtgc
      541 ggcggcgagc ttcatgttct cggccttgct gaagtcgcgc atctcccacg ccaggtacac
      601 cagtgtgagt ggcccaacga tgtacatgaa catgtccgtg taagcggtgt tggtcaccat
      661 caccaggatc agggggatca ccaggagcga gccgatgtag gtgccccatt cgtacagctt
      721 gcgcttgttg gcgggcatgt ccgggtgcaa ggggctgagg ccgatggggc cgaaggtctt
      781 ccgacggaag gcgaagttga tcaggctgat gatcatcacc acgcccacca gggcgaaggc
      841 cgcacgccag gagtaggatt tgcccaccca caccatcagc aggccaccca ggaaggcgcc
      901 gatgttgata cccatgtaga agagcccgaa acccgcatcg cgccggtgat cgccgtcgtt
      961 gtacaactgg ccgaccatgg tgctgatgtt cggtttgaag aaacccgttc cgatgatgtt
     1021 gaagcagatg ccgatgtaga acagctcctt gggcgccgca gcgatcacga agccacccac
     1081 gatcatcagc aaggcccccc agaacagcga cttctggaat ccgaggatct tatcggcgaa
     1141 tacgccgccc agaaaggtga aggcgtacac gaaggcctgg atggccccgt actgcaggtt
     1201 cgcctgcttc tccggcatcg ccagttcggt agccatgaac accaccagca tgccccgcag
     1261 gccatagaag cagaagcgct cccacatctc cgtgccgaag aggtagaaga gctgcttggg
     1321 gtaagtgccc ttgaaatcgc ggatctgttg cagggtgaga aggccgctca tgggtgcgtg
     1381 atgatgtggt ggtgatcctg gtgctgacag cgcggatcag tggatgttct tctcgcgcat
     1441 cacggcattc aaccagcgca gcaggaagaa gagcatg
//
LOCUS       DAOQOC010000906         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71098_length_1477_cov_3.063994, whole genome shotgun sequence.
ACCESSION   DAOQOC010000906 DAOQOC010000000
VERSION     DAOQOC010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71098_length_1477_cov_3.063994"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PL106_12975"
     CDS             complement(<1..267)
                     /locus_tag="PL106_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34031.1"
                     /translation="METAVGSSWKQRGTGILAFVIVLLLMPLGHALMVLLEMLGTAVF
                     ASALIVGFLGCVMLWWGVARPMRDLYATLLGAIGGVLTWTGWVEF"
     gene            complement(461..637)
                     /locus_tag="PL106_12980"
     CDS             complement(461..637)
                     /locus_tag="PL106_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34032.1"
                     /translation="MELLREPKEVDFYVEDRPLTDDEKRLLKEAIAKNKAKAKRAKSK
                     RAATSRRTAPASKR"
     gene            complement(641..1159)
                     /locus_tag="PL106_12985"
     CDS             complement(641..1159)
                     /locus_tag="PL106_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34033.1"
                     /translation="MSNEKRRIGVVIDKLTNSIELVATGDVFNTIITRATTADKRLIK
                     RKEWLFDWHREIANTKREVYRLMTENNADIIQGLICLEDKGDHLYMHLIESAAFNRGR
                     RKTFLGVPGNLIAYACKLAFDRGHDGFVAFTAKTALVEHYRKELGATVIHGTLMHLDS
                     LAARNLVQRYKL"
     gene            complement(1156..>1477)
                     /locus_tag="PL106_12990"
     CDS             complement(1156..>1477)
                     /locus_tag="PL106_12990"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNA34034.1"
                     /translation="CSFVPRLYDYHPQAIPAPYNHSNIDSDEVLYYVAGDFMSRNDIG
                     PGHISLHPAGIPHGPHPGAMERSIGKKATDELAVMVDTFKPLMVTEEAMKIDDGKYWK
                     SWLE"
BASE COUNT          264 a          462 c          426 g          325 t
ORIGIN      
        1 gaactcgacc cagccggtcc aggtgagcac cccgccgatg gcgcccagca gcgtggcgta
       61 caggtcgcgc atgggccgtg ccacgcccca ccagagcatc acgcagccca ggaagcccac
      121 gatgagcgca ctggcgaaca cggcggttcc cagcatttcc agcagcacca tcaaggcatg
      181 cccaaggggc atcaacaaca gcacgatcac gaaggccagg atccctgtgc cccgttgttt
      241 ccacgaactt cccacggcgg tctccatcgt gggtgcaaag tgacactacg gtgacaaagc
      301 gggcaatacc acgatcaggt catttcgggg gtgggagccg ctgatcggat caacttcact
      361 ccacccagct cttccggtac ttcccgtcgt cgatcctcat cgcttcttcg gtcaccatca
      421 gcggcttgaa ggtgtccacc atcacggcga gctcgtcggt ctagcgcttt gaagcaggcg
      481 ctgttctgcg agaagtagct gctcgcttgg acttcgcacg tttcgccttc gctttgttct
      541 tggcgatggc ttctttcagc aaccgcttct catcatccgt gagcggacgg tcctctacat
      601 aaaagtccac ctctttcggt tcgcgcagca gttccatggt tcaaagcttg tatcgctgca
      661 ccaagttacg cgctgcaagg ctgtccaggt gcatcagtgt gccatggatc acggtcgccc
      721 ccagttcctt ccggtagtgt tcgacaagtg cggtcttggc cgtgaacgcc acgaagccat
      781 cgtgcccgcg gtcgaaggcg agcttgcaag cgtaagcgat gaggttgccc ggcacgccaa
      841 ggaaggtctt gcgccgacca cgattgaagg cagcgctctc gatcaggtgc atgtacaggt
      901 ggtcaccctt gtcctccagg caaatgagac cttggatgat gtccgcgttg ttctcggtca
      961 tcagccggta tacttcacgc tttgtgttcg cgatctcccg atgccagtcg aacagccatt
     1021 ccttccgctt gatcaatcgt ttgtccgctg tcgtggcccg cgtgatgatg gtgttgaaca
     1081 catcacctgt ggcaacaagc tcgatggagt tcgtgagctt gtcgatgacg accccgatgc
     1141 gtcgcttctc gttgctcact ccaaccagct cttccagtac ttcccgtcgt cgatcttcat
     1201 cgcttcctcg gtcaccatca gcggcttgaa agtgtccacc atcacggcga gctcgtcggt
     1261 ggccttcttg ccgatgctgc gctccatggc gcccgggtgc ggaccgtgcg ggatgccggc
     1321 ggggtgcaag ctgatgtggc ccggaccgat gtcgttgcgg ctcatgaaat cgccggccac
     1381 gtagtagagc acctcgtcgc tgtcgatgtt gctgtggttg tacggcgcgg ggatggcctg
     1441 cgggtggtag tcgtacaggc gcggcacgaa gctgcac
//
LOCUS       DAOQOC010000907         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71114_length_1477_cov_2.580872, whole genome shotgun sequence.
ACCESSION   DAOQOC010000907 DAOQOC010000000
VERSION     DAOQOC010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71114_length_1477_cov_2.580872"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..>1477)
                     /locus_tag="PL106_12995"
     CDS             complement(424..>1477)
                     /locus_tag="PL106_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34035.1"
                     /translation="DGSLDTSYGTSGISLCNPTTGADYGLGLQLMDDGSTLQFGYSTT
                     NVSLVVKRTPAGQLDAGFGTNGVLTIPALVSEMNINGGTMINDGSILLHGLGVWPAWS
                     ALIVKLTTDPSAGLFADLGPDTGFCPGSTVVLDAGSPGSTYQWNMSFQNLGTTQTITA
                     SSGGAYNVTITDAQGCTDRDTINVTAWPAPLTPQIFGDAFELFTSALPEIQWYLNGEP
                     IPGANEEIWIPQQNGTYTVTETAPNGCSSTSAPFDVLNVGIVEPGHSNLAIQVAPVPI
                     SEASVVTFRTTRSEKASMHLHDATGRVVLTVFADRYMPAGMQRLTLDKLPDLAPGKYF
                     LSLTSNSGRYTVPVVR"
BASE COUNT          264 a          397 c          499 g          317 t
ORIGIN      
        1 aggtgtgggt cacggtattg ttgggggtag tgctgccgac cgtggtgagg atgggatcgc
       61 ggtcgttgcc gctgccggtg tattcgatct ggccgttgaa agtggcatcg cccgcccaga
      121 gcactcgagc ggggaaggcg ccggtgatgc tcgtacggcc aggtccgtga gcgatgttcc
      181 cggttcctgg acggcgagca ggaggaaaag gaggatcaga acgtggcgcg ttttcatgtg
      241 gacgttgcgc ttgggttgcg tgaacaaagg cgtatatcca acaggtaccc tttcttgagc
      301 aggctggaca ataccgggta tcctggttcc gatccgttgt atggatgtga tgcgaggcgc
      361 cgtcaacccc ccgcgctcat cgcagaggtt gacggttttc atctctgctc gggccgctcg
      421 gtctcaacgc accacaggca cggtataacg tcccgaattg gaggtaaggc tcaggaagta
      481 cttgcccggc gccaggtccg gcagcttgtc gagcgtcagg cgctgcatac cggctggcat
      541 gtatcggtcg gcgaagaccg tgagcacgac acgtccggtg gcatcgtgca ggtgcatgga
      601 ggccttttcc gatcgtgtgg tccggaacgt caccaccgag gcctcggaaa tgggaaccgg
      661 tgcgacctgg atcgccaggt tggaatgtcc cggttcgacg atgcccacgt tcagcacgtc
      721 gaacggtgct gatgtgctgc tgcatccgtt gggagcggtt tccgttaccg tgtacgtgcc
      781 gttctgttgt gggatccaga tctcctcgtt cgctcctggt atcggctcgc cattgaggta
      841 ccactggatc tccggtaagg ccgaggtgaa gagttcgaaa gcatcgccga agatctgggg
      901 agtgagcggc gcgggccaag cggtcacgtt gatggtgtcg cgatccgtgc aaccttgtgc
      961 atcggtgatg gtaacgttgt acgcgccgcc cgaactcgcg gtgatggtct gcgtggtgcc
     1021 gaggttctgg aagctcatgt tccattggta ggtgctgccg ggggaacccg cgtcgagcac
     1081 cacggtgcta cctgggcaaa agcccgtatc aggacccagg tcggcgaaga ggccagcgct
     1141 cgggtcggtg gtgagtttta cgatcagggc cgaccatgcg ggccacacgc caaggccatg
     1201 cagcagaatg ctgccatcgt ttatcatggt gccgccattg atattcattt cgctcaccag
     1261 cgcggggatg gtcaacacac cgttcgttcc gaaaccggca tcgagctgcc cggccggtgt
     1321 gcgcttgacc acgagtgaaa cattggtggt ggagtagccg aattgcaagg tggatccatc
     1381 gtccatcagt tgaagtccta agccgtagtc tgcgccggtg gtcgggttgc acaggctgat
     1441 gccacttgtg ccgtacgatg tatcgagcga accgtcc
//
LOCUS       DAOQOC010000908         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71246_length_1475_cov_4.165493, whole genome shotgun sequence.
ACCESSION   DAOQOC010000908 DAOQOC010000000
VERSION     DAOQOC010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71246_length_1475_cov_4.165493"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PL106_13000"
     CDS             complement(<1..649)
                     /locus_tag="PL106_13000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA34036.1"
                     /translation="MKTLSIVIPVYNGAGSIERLTHELARLNVDGGVELVLVNDCSPD
                     DSWEVIQRLVKERPMPIVAVDLARNFGEHNAVMAGYAASSGKFIINIDDDFQNPPGEV
                     VKLLGYAQAHPEFDVVYTKYARKRHGFFRNLGSRFNDRVANFLLDKPKGLYLSSFRCV
                     NRFLCDGILEYTGPYPYIDGIILERTQRIGTLEVMHAPRKEGRSGYTLRKLIRRDL"
     gene            complement(646..1392)
                     /locus_tag="PL106_13005"
     CDS             complement(646..1392)
                     /locus_tag="PL106_13005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA34037.1"
                     /translation="MSRTGMESVEYTQMELVEETMWWYQGLHANILDALGKFAPDAKN
                     LLDAGCGTGGMLKTIRERFPSISLHGTDISEQACIAARAKSGADVLLGSVDALHYPDR
                     SFDALISLDVLGYDLDVDAAVNGFFRVLRTGGYAILNLAAYQWMLSYHDKAVGQSHRY
                     TRSAAVGLFRKHGFTVHFAGYWNTILFPLMVAQRKLMRSSGASDVRPFHPLVNNAFKT
                     CLAVERAFIRRGVALPFGGSLLIVVQRPPQ"
BASE COUNT          296 a          451 c          445 g          283 t
ORIGIN      
        1 tgaggtcgcg gcggatcagc ttgcggagcg tgtagccgct gcggccctct ttgcgcggtg
       61 cgtgcatcac ctccaacgta ccgatgcgct gcgtacgctc cagaatgata ccgtctatgt
      121 acggataagg cccggtatac tccaggatgc cgtcgcacag gaagcggttc acgcagcgga
      181 aactggacag gtagagcccc ttgggcttgt ccaggaggaa gttcgccacg cgatcgttga
      241 aacggctgcc caggttgcgg aagaaaccgt gccgcttgcg tgcgtacttc gtgtagacca
      301 cgtcgaactc aggatgcgcc tgcgcgtacc ccaggagttt gaccacctcg cccggtggat
      361 tctggaaatc atcatcgata ttgatgatga acttgccgct gctcgcagcg tagcccgcca
      421 tcacggcgtt gtgctccccg aaattgcgcg cgagatccac ggccacgatg ggcatgggcc
      481 gctccttcac caatcgctgg atgacctccc agctgtcgtc agggctgcag tcattcacaa
      541 gcaccagttc cacgccgccg tccacgttca gccgggccag ttcgtgtgtg agccgctcga
      601 tgctgcccgc accattgtac acggggatca cgatggagag tgtcttcact gcggggggcg
      661 ttgtacgacg atcagcaaag atccaccgaa aggcaaggct actccgcggc ggatgaaggc
      721 gcgttccacg gccaggcagg ttttgaacgc gttgttcacc aggggatgga acggacgcac
      781 atcactggcc ccggaagagc gcatcagctt gcgttgcgcg accatgagcg ggaacaggat
      841 cgtgttccag taaccggcga aatggaccgt gaacccatgc ttcctgaaaa gtccgacggc
      901 tgcggatcgc gtatagcggt gagattgccc gaccgccttg tcatggtagg acagcatcca
      961 ctggtaggcc gccaggttca ggatggcgta gcctccggtc cggagcacac ggaagaaacc
     1021 gttgacggcc gcgtccacat ccagatcata gcccagtaca tccaaggaga tcaacgcatc
     1081 gaaggaccgg tcaggatagt gcagcgcatc cacggagccg agcagcacat cggcgccgga
     1141 cttggcacgt gccgcgatgc aggcctgctc ggaaatatcg gttccatgca aggagatgga
     1201 cgggaaccgc tcgcgtatgg tcttcaacat gccgccggtt ccacaaccag cgtcgagcag
     1261 gtttttcgca tcgggtgcga acttccccaa cgcatcaagg atgttggcgt gcagtccttg
     1321 gtaccaccac atcgtctcct cgacgagttc catctgcgtg tactccacgc tctccatgcc
     1381 ggtcctgctc attccccgcg cttgtaagcc ttgtaggcgt tgcggtcgcg catgtagtcc
     1441 tcctcggcgt acggcaggtc gcagaacacc gcgat
//
LOCUS       DAOQOC010000909         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71272_length_1475_cov_2.923944, whole genome shotgun sequence.
ACCESSION   DAOQOC010000909 DAOQOC010000000
VERSION     DAOQOC010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71272_length_1475_cov_2.923944"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..715
                     /locus_tag="PL106_13010"
     CDS             2..715
                     /locus_tag="PL106_13010"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNA34038.1"
                     /translation="MTRLSVNVNKLATLRNARGGNNPDVVAWARAIERYGAQGITVHP
                     RPDERHIRRSDVRALSKAITTEFNIEGYPSEDFIALVLEVRPAQVTLVPDPPGVLTSN
                     AGWDAREHKMFLKEVLARFKREGIRTSVFMGTDPEQLSHAADTGTDRVELYTEPYAAN
                     YHKDRASAVAPFANAAEKARQLGLGLNAGHDLDLHNLAFFNANVPGLQEVSIGHALVC
                     DALMYGMENTVGLYLRELR"
     gene            712..>1475
                     /locus_tag="PL106_13015"
     CDS             712..>1475
                     /locus_tag="PL106_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA34039.1"
                     /translation="MSVDLFHRRAGQGTPIVILHGLFGSSDNWASIGRELEKDHDVLL
                     VDQRDHGRSPHTNEVNYGLMADDVQRLVTGQGLQNIVLVGHSMGGKTAMWFAHRWPGL
                     VKHLVSVDMGPREYPISNQAPIIEALLTSDLDRKTSRKEVDEHLARYVPEPGVRQFLL
                     KSLYWETPEKLAWRMNVPILARDIHHILGAAPPELIRVPALFIRGGQSDYITRGDVPA
                     IKEQFPNSRIETVEFAGHWVHAQAPTEVMDLIRSVA"
BASE COUNT          303 a          468 c          469 g          235 t
ORIGIN      
        1 gatgacccgc ctgagcgtta acgtgaacaa gttggccacc ttgcgcaacg cgcgcggcgg
       61 caacaacccc gatgtggtgg cttgggcacg ggccatcgag cggtacggcg cgcagggcat
      121 cacggtacac ccccggcccg atgaacgcca catccgacgg tccgatgtac gcgccctgag
      181 caaggcgatc accacggagt tcaacataga gggatacccc agcgaggact tcatcgcctt
      241 ggtactggag gttcggcccg cgcaggtgac cctggtgccg gatccgcccg gtgtgctcac
      301 cagcaacgcg ggctgggatg cacgcgaaca caagatgttc ctgaaggaag tgctggcccg
      361 cttcaagcgt gaaggcatcc gtaccagcgt gttcatgggt accgaccccg agcagctcag
      421 ccatgcggcc gataccggca ccgatcgcgt tgaactctac accgagccat acgccgccaa
      481 ctaccacaag gaccgcgcat cggcagtggc gcctttcgcc aatgcagcgg aaaaggcgcg
      541 gcaactcggc ctgggcctca acgccggaca tgatctcgac ctgcacaacc tggcgttctt
      601 caacgcgaac gtgcccgggc tgcaagaagt gagcatcggc cacgcgctgg tgtgcgacgc
      661 gctgatgtac ggcatggaga acacggtggg gctgtatttg cgggaattgc gatgagtgtc
      721 gatctcttcc atcgccgcgc cggacagggc acaccgatcg tgatcctgca cggcctcttc
      781 ggcagcagcg acaactgggc cagcatcggc cgggaactcg aaaaagacca cgacgtgctg
      841 ctggtggatc aacgggatca tggccgttca cctcacacca acgaagtgaa ctacggattg
      901 atggccgacg atgtgcaacg gttggtcacg ggccagggcc tgcagaacat cgtgctggtg
      961 ggccacagca tgggtggcaa aacggcgatg tggttcgcac accgctggcc ggggctggtg
     1021 aagcacctgg tgagcgtgga catgggtccg cgcgagtatc ccatcagcaa ccaggcacct
     1081 atcatcgagg cgttgctgac gagtgacctg gaccgcaaga ccagccgcaa ggaggtggat
     1141 gagcacctgg cgcgctatgt gcccgagccg ggcgtgcgcc agttcctgct caaaagcctc
     1201 tactgggaga cgcctgaaaa actggcctgg cgtatgaacg tgccgatcct ggcacgcgac
     1261 atccaccaca tcctcggcgc cgccccgccc gaactgatcc gggtgcccgc cctcttcatc
     1321 cggggtggcc agagcgatta catcacccgg ggcgatgtac cggcgatcaa ggaacaattc
     1381 ccgaacagcc gcatcgagac cgtggagttc gccggccact gggtgcatgc gcaggcccct
     1441 accgaggtga tggacctgat ccgttccgtg gcatg
//
LOCUS       DAOQOC010000910         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71367_length_1474_cov_3.250176, whole genome shotgun sequence.
ACCESSION   DAOQOC010000910 DAOQOC010000000
VERSION     DAOQOC010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71367_length_1474_cov_3.250176"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1474)
                     /locus_tag="PL106_13020"
     CDS             complement(<1..>1474)
                     /locus_tag="PL106_13020"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA34040.1"
                     /translation="QDLFDALPHEPELERYGALLRSTGQGRVIAGFSGEPGVPLDSLS
                     ISADRFTERLLHAHPDLIASAFTRPDPDVFEDAVASIHAQLPVYADAQVLARVARMDS
                     LAVDSAIGAVRNRLASFSGELELNNVLADPFGLSTPLIGRLMASGRMAGITLLNGQLF
                     TPDSTLAIVVITLRSEDHRVLDTLNTAIAQACAPGVAGAVFGSVPMAAVNARRITTDA
                     TNTSLLALVLIVAILIWWYRSWRIPILFTIPPAIGFVLGWGALAWSRPGISSLSLGAG
                     AALLGIAFDYCFHFFTHLRHRRDIAATLREISAPMLLGCTTTVLAFAALSFTGSRILA
                     DLGIVAVCMLIGAALTVLLVLPHFVAVPLPVEAEHAPPRAPGKRSLWGLAFVVVATCA
                     LVPFASHVEFDGDPERISWIPDDMRALRDRLEGEKDRLVPVLVEGHASSADEARVLLE
                     RATAHVRHAGHDSLVPLMPTDLHPSGSGVTERMARWDAVFG"
BASE COUNT          293 a          470 c          503 g          208 t
ORIGIN      
        1 ctccgaacac ggcatcccag cgggccatac gttcggtcac gccggatccc gaaggatgca
       61 ggtcggtagg catcagcggt accaggctgt cgtgtccggc atgccgcaca tgggcggtgg
      121 cccgctccaa cagcacgcga gcctcatcag cggatgacgc atggccttcc acgagcacgg
      181 gcaccaggcg gtccttctcc ccttcgagcc gatcgcgcag cgcgcgcatg tcgtccggga
      241 tccagctgat gcgttcggga tcaccatcga attccacgtg cgaggcgaac ggaacgagtg
      301 cgcaggtggc cacgaccacg aaggcaaggc cccacaggct gcgcttgccg ggggcccttg
      361 gcggcgcgtg ttccgcttcc acgggcaacg gcacggccac aaaatgcggt aataccagta
      421 atacggtaag tgcggcgccg atgagcatgc acacggccac gatgccgaga tcggccagga
      481 tccggctccc ggtgaaggac aaggccgcga aagcgagcac ggtggtggtg cagcccagga
      541 gcatgggtgc cgagatctcg cgcagggtgg ccgcgatatc ccgccgatgg cgcaggtgcg
      601 tgaagaagtg gaagcagtag tcgaacgcga tgccgagcaa ggcggcaccc gcacccagtg
      661 aaagcgagga gataccgggg cgggaccatg ccagggcgcc ccagcccaga acgaaaccga
      721 tggccggtgg aatggtgaag aggatcggta tgcgccagct gcgataccac cagataagta
      781 tagccacgat gagcacaagt gcgaggaggc tcgtgttggt ggcgtcggtg gtgatgcgtc
      841 gcgcgttcac cgctgccatg ggcactgaac cgaacacagc gcccgcgacc ccgggcgcac
      901 aagcctgcgc gatcgcggtg ttcaacgtat cgagcacccg gtggtcctca ctgcgcaggg
      961 tgatcaccac gatggccaat gtgctgtccg gtgtgaagag ctgcccgttc agcagggtga
     1021 tcccggccat gcgcccggag gccatcaacc gaccgatcag cggcgtggac aggccgaagg
     1081 ggtcggccag gacgttgttc agttccagct cgccgctgaa ggaggccagg cggttccgca
     1141 ccgcaccgat cgcgctgtcc acggccaggc tgtccatccg ggccacgcgg gccaacacct
     1201 gcgcatcggc gtacacgggc agttgcgcat ggatcgaagc gaccgcgtct tcgaacacat
     1261 cgggatcggg gcgggtaaaa gcgctggcga tcaggtccgg atgtgcgtgc agcaaccgct
     1321 cggtgaaccg atcggcactg atcgagaggc tgtccagcgg aacacccggc tccccgctga
     1381 aaccggcgat cacgcgcccc tgcccggtac tgcgcagcag cgccccgtag cgctccaatt
     1441 ccggctcgtg gggaagcgca tcaaaaaggt cctg
//
LOCUS       DAOQOC010000911         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71479_length_1473_cov_2.827927, whole genome shotgun sequence.
ACCESSION   DAOQOC010000911 DAOQOC010000000
VERSION     DAOQOC010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71479_length_1473_cov_2.827927"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PL106_13025"
     CDS             <1..216
                     /locus_tag="PL106_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNA34041.1"
                     /translation="NLCPADRKDQPVLGMEIIRDMVKDDDEWEDGTILDPENGKVYDC
                     KLWLEDGKLMVRGYVAFFYRTQEWVRE"
     gene            511..1311
                     /locus_tag="PL106_13030"
     CDS             511..1311
                     /locus_tag="PL106_13030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNA34042.1"
                     /translation="MNVTKALFHLFEHAYRLGWNRAVHVIRLKRRGPGGLQTVDAPWL
                     GGKVYVRPSTADMATIDQFLIGPYMPATTGAPPRNVVDCGANIGLATRYLKQLFPQAR
                     IIAIEPDGENFELLRKNIEHLQGCEAVQAAVWPMDGFVELERDGLRHSAFRTRSTTGG
                     GDSIEALSIPRIMERFGLDRIGLLKIDIEGAEMELFRDPDLGWLERVDRIAIELHDLF
                     KPGCGDAFFKALSRSAWTYRFYGEVVYCERITTTGATPSPLAGVATAS"
BASE COUNT          299 a          433 c          452 g          289 t
ORIGIN      
        1 aacctctgcc cggccgaccg taaggaccag cccgtgctcg gcatggagat catccgtgat
       61 atggtcaagg atgatgacga gtgggaggac ggcaccatcc tggaccccga gaacggcaag
      121 gtgtacgact gcaaattgtg gttggaggac ggcaagttga tggtgcgcgg gtatgtggcg
      181 ttcttctacc ggacgcagga gtgggtgagg gagtgatcta tcgcaagagg gtcacgtgcc
      241 ccggacggtg aatcatgcgt ccgctctgac attggtccgc ataccgcagg acatagacgt
      301 aggtaccatc aggaacaggc ttatccttgt aggtaccatc ccaggccctt tcggtctccg
      361 tgctattgaa gatgcgttca ccccatcgat tgaacacgga cagctcgtat cgactatcct
      421 ccgcaccgaa cggacccacc acccaactcg cgatgcatgc ggaacccgca ccggtcatcc
      481 gggcattccg aaggttagtt tcgcgcctcc atgaacgtca caaaagcgct cttccacctg
      541 ttcgaacatg cttaccgtct cggttggaac cgggccgttc atgtgatccg gctgaagcgg
      601 cggggtccag gagggctaca gacagtggac gcgccctggt tgggtgggaa ggtgtacgtg
      661 cgcccgtcca ctgcggacat ggccaccatc gaccagttcc tgatcggtcc gtacatgccc
      721 gcaacaacgg gtgcgccgcc caggaacgtg gtggattgcg gggccaacat cggtctggcc
      781 acccggtacc tgaagcagct ctttccacaa gcacggatca tcgccatcga accggatggg
      841 gagaatttcg aactgctgcg gaagaacatc gaacacctgc aaggctgcga ggcggtccag
      901 gccgccgtgt ggcccatgga cggtttcgtg gaactcgagc gcgatggcct ccggcattcg
      961 gcattccgga ccagatcaac gaccggtggc ggcgacagta ttgaagcgct ctccataccc
     1021 cggatcatgg agcgattcgg cttggaccgg atcgggctgc tgaagatcga catcgaaggt
     1081 gccgaaatgg aactcttccg cgatcccgac cttggttggc tggaacgcgt ggatcgtatt
     1141 gctatcgagt tgcacgatct gttcaaaccc ggctgcgggg atgccttctt caaagccttg
     1201 agtcgttcgg cctggaccta cagattctac ggcgaagtgg tctattgcga gcggatcacc
     1261 acaacgggag caaccccttc acccttggcc ggtgtggcga cggcctcctg atccggaact
     1321 tgccgatgga ccgtgacggg aacggatcga cgcacgggag gcctgtcatc ctttattgct
     1381 acccgaggcg ttccacattc atcgaacgcg acgtgtcggg gcttgccgcc tcatttgaag
     1441 tgcgcacgaa tgaactgcgc gcggacccgg cct
//
LOCUS       DAOQOC010000912         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71866_length_1469_cov_3.402405, whole genome shotgun sequence.
ACCESSION   DAOQOC010000912 DAOQOC010000000
VERSION     DAOQOC010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71866_length_1469_cov_3.402405"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PL106_13035"
     CDS             complement(<1..598)
                     /locus_tag="PL106_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34043.1"
                     /translation="MSETADKTVRLSKVAREFNQALHTIVEFLASKGHKVDANPNAKI
                     GSVEYDLLNAQFGTDKAEKEASKQIVQARHDRESVVMASGGHKEAPVERRTPAPAPAP
                     EPVAEAPPAPTPTAPAPAETPVAATPVTATPAGPEVIKARAEKVDGPKTLGKIDLDAQ
                     KRTSKKTSAQREAKVEPEPAPPPAAPAGESIAPPAAVPP"
     gene            complement(709..>1469)
                     /gene="nusA"
                     /locus_tag="PL106_13040"
     CDS             complement(709..>1469)
                     /gene="nusA"
                     /locus_tag="PL106_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNA34044.1"
                     /translation="NELIMPKDQQIKTDFFKKGDNVRAVVWKVEMYNNSPRVILSRTA
                     PEFLEKLFEQEVPEVADGLITIKRIVREPGERAKVAVESYDDRIDPVGACVGMKGSRI
                     HGIVRELRNENIDVINWTANEPLLIQRALSPAKIGNIKLDEPNKRAEVYMKPDQVALA
                     IGKGGHNIKLAGRLTGYDIDVYRDSDEVTDDVDLDEFSDEIETWIIDELKKVGLDTAR
                     SVLDLGREELVKRTDLEEETIDDVLRVLRTELAK"
BASE COUNT          189 a          485 c          437 g          358 t
ORIGIN      
        1 gcggcggtac tgctgccgga ggagcaatgc tctcaccagc aggtgctgca ggaggtggcg
       61 cgggttccgg ctcgaccttc gcctcgcgct gcgcggacgt tttcttcgat gtgcgcttct
      121 gcgcatccag gtcgatcttg ccaagggtct tcggaccgtc gactttttcc gcccgggctt
      181 tgatcacctc ggggccggca ggcgttgcgg tcaccggtgt tgccgcaacg ggtgtttctg
      241 cgggcgccgg tgcagttggg gtgggtgcag gcggcgcctc ggccaccggt tctggtgctg
      301 gtgcaggggc gggagtgcga cgttcaacgg gagcttcctt gtgcccaccg ctggccatca
      361 ccacgctctc gcggtcgtgc cgggcctgaa cgatctgttt gctcgcctct ttctcggcct
      421 tgtcggtgcc gaactgcgca ttgagcaggt cgtactccac cgacccgatc ttggcgttcg
      481 ggttggcatc caccttgtgg cccttggagg ccaggaactc cacaatggtg tggagcgcct
      541 ggttgaattc ccgcgccact ttgctcagtc gcaccgtctt gtcagccgtt tcactcatac
      601 ggtcccttgg cgagccaagg ctccttggtt tttgttcctc ttcaacttcc ggtggcgtcg
      661 ggccgccacg taccatccaa ccatgtccgt tgccgtatcc accgatcctt atttggccaa
      721 ttccgttctc aatacccgaa gcacatcgtc gatggtctcc tcctccaggt ccgtacgctt
      781 caccagttcc tcgcggccca gatccagcac gctgcgggcg gtgtccagcc ccaccttctt
      841 cagttcatcg atgatccagg tctcgatctc gtcgctgaat tcgtccaggt ccacgtcatc
      901 ggtcacctcg tcgctgtcgc ggtacacgtc gatgtcgtat cctgtgaggc ggccggccag
      961 cttgatgttg tgcccgccct tgccgatggc cagcgctacc tggtcgggtt tcatgtacac
     1021 ttcggcgcgc ttgttcggct catccagctt gatgttgccg atcttggccg gactgagggc
     1081 ccgctggatg agcaggggct cgttcgcggt ccagttgatc acgtcgatgt tctcgttgcg
     1141 cagctcgcgt acgatgccgt ggatgcggga tcctttcatg cccacgcacg cgcccaccgg
     1201 atcgatgcgg tcgtcgtagc tctccaccgc caccttggcg cgctcgcccg gctcgcgcac
     1261 gatgcgcttg atggtgatca ggccgtcggc cacttccggc acctcctgct cgaacagctt
     1321 ctccaggaat tccggtgcgg tgcggctcag gatcacccgc gggctgttgt tgtacatctc
     1381 caccttccac accaccgcgc gcacgttgtc gcccttcttg aagaagtccg tcttgatctg
     1441 ctggtccttg ggcatgatca attcgttgc
//
LOCUS       DAOQOC010000913         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_71871_length_1469_cov_3.268034, whole genome shotgun sequence.
ACCESSION   DAOQOC010000913 DAOQOC010000000
VERSION     DAOQOC010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71871_length_1469_cov_3.268034"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PL106_13045"
     CDS             <1..260
                     /locus_tag="PL106_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA34045.1"
                     /translation="SDVIIPNVFSPDGDGHNDALEFENAQYFNNNHLRVYNRWGQVVY
                     ESTSYKNNWRPRDVPEGTYYFVFAMQDGRKWAGHVTLLRTN"
     gene            388..>1469
                     /locus_tag="PL106_13050"
     CDS             388..>1469
                     /locus_tag="PL106_13050"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNA34046.1"
                     /translation="MRIILLPFTAALAIACQGQVLLSDSLLQTLSAGELGGQGIPSPQ
                     YGVSIHKLVYATTDAFGAATAASGALVLPNAPWCDLPLFAYDHGTVTGRNEVPSRLSY
                     EILVGKYMGSSGFVSVLPDYLGLGDSPGMHPYIHAVTEATATIDMLRAAREYCSVHGI
                     GLNGEVFLAGYSQGGHAAMATHRMIQEQFPDEFNVVASAPCSGPYDCSGVQASVITAD
                     ESYPAPYYLPYILLAYKDIYPGLPPAMADIFVTPYDTLLPPLFDGTHSSGDIDAVMPD
                     TPNLVVQPALLAAYMSDPDHPFRMALRDNDVYDWAPAAPMRLNYCSGDHHVFYMNSIV
                     ARDTMLANGATGVTATDLGPGTEHQS"
BASE COUNT          284 a          483 c          432 g          270 t
ORIGIN      
        1 cgagcgacgt gatcattccg aacgtgttca gccccgatgg cgacgggcat aacgatgcct
       61 tggagttcga gaacgcgcag tacttcaaca acaaccattt gcgggtgtac aaccgttggg
      121 gacaggtggt gtacgagagc accagctaca agaacaactg gcgcccacgc gatgtaccgg
      181 agggcacgta ctacttcgtg ttcgccatgc aggatgggcg caagtgggcg gggcacgtca
      241 ccttgctgcg cacgaactga tcgaactgtc acgaggtatc caggcgcagg agcaggaatt
      301 gatcctgcgg ggcctccgga ccgtttttcc attccgacag ggagcatagt ttcggcacgc
      361 ccggccccat gagcccggcc agcccgcatg cgcatcatcc tgctcccatt cactgccgcg
      421 ttggccattg catgccaagg acaggtattg ctctccgact ccctccttca aacgctctca
      481 gcgggtgagt tgggcggcca gggcatacca tcgccccagt acggcgtgag catccacaaa
      541 ctggtgtacg cgacaaccga tgccttcggg gcggccaccg ccgccagtgg tgcactggtg
      601 ctgcccaacg ccccatggtg cgatctgccg cttttcgcat acgaccatgg aacggtgacc
      661 ggacggaacg aagtaccctc ccgcctgagc tacgagatcc tggtgggcaa gtacatgggt
      721 tcctccggct tcgtcagcgt gctgcccgat tacctgggcc tgggcgattc acccggcatg
      781 cacccataca tccacgcggt caccgaggcc acagccacca tcgatatgct gcgggccgcc
      841 cgtgaatatt gctcggtaca cggtatcggg ctcaatggag aagtgttcct cgcgggttac
      901 tcgcagggcg ggcacgcggc catggccacg catcggatga tccaggagca gttcccggac
      961 gaattcaacg tggtggccag cgcaccgtgc agtggaccgt acgactgcag cggggtgcaa
     1021 gcctcggtga tcacggcgga tgagtcctac ccggccccct actacctgcc gtacatcctg
     1081 cttgcctaca aggatattta tcccggcctg cccccggcca tggccgacat cttcgtgaca
     1141 ccgtacgaca ccctgctccc accgctcttc gatggcacgc actcctccgg cgatatcgat
     1201 gcggtgatgc ccgacacgcc gaacctggtg gtacaacccg cgttgctggc ggcttacatg
     1261 agcgacccgg accatccgtt ccgcatggca ctgcgcgaca acgatgtgta cgactgggct
     1321 cctgcggctc ccatgcgcct gaactactgc agcggggacc atcacgtgtt ctacatgaac
     1381 tccattgtgg cacgcgatac gatgctggcc aacggagcaa ccggtgtaac ggccacggat
     1441 ctcggtccgg gcaccgagca ccagtcctg
//
LOCUS       DAOQOC010000914         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_72241_length_1466_cov_2.588944, whole genome shotgun sequence.
ACCESSION   DAOQOC010000914 DAOQOC010000000
VERSION     DAOQOC010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72241_length_1466_cov_2.588944"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="PL106_13055"
     CDS             complement(<1..1420)
                     /locus_tag="PL106_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA34047.1"
                     /translation="MRTEAEFYSAICAVRLSNDDAGQRLQAFLEEHPESFRTGAVNLE
                     LFRNWFNQKEWKEALAFAMKVDDLGLSQSGRDEFHFKRGYAYFQEEQVDPALAEFSKV
                     KDGSSAYAPSATYYTAHINYTRKNYTAALEGFEKLQSDEAFARTVPYYIAQIKFLQGD
                     YNALLEYTKPLLAEGGSVKSKGDINRLAGEAYYRTGQFKEAQPYLEKSVQGTGVERGD
                     RYIAGYVFYKNGDCKKALAQFNLVASPGNQPVEDSLAQLAAYHMADCYLSLNEKNYAR
                     NAFKRAYDIGVDQRVTEDALFNYAKLAYELSFDPYNEAIIALRDYLKAYPNSGRHDEA
                     QQFLMNVYLKTRNYEAALQALDAIRDKDPRLQEVYQELAFNRGVELYEGHKYAEAVPF
                     FQKALVFPVDKEVNARAHYWEGECRYAMGEYGEALKKYDDIRNSSGAYATDLYEEASY
                     SQGYCYFKLKEYAEAATSLRRYT"
BASE COUNT          227 a          459 c          441 g          339 t
ORIGIN      
        1 cggtgtaccg ccgcaacgag gtggcggcct cggcgtattc cttcaacttg aagtagcaat
       61 agccctggct gtagctggcc tcttcgtaca ggtccgtggc gtacgcgcca ctgctgttgc
      121 ggatgtcgtc gtatttcttc agcgcctccc cgtattcgcc catggcgtac cggcactccc
      181 cttcccagta gtgcgccctc gcattcacct ccttgtccac cgggaacacc aacgccttct
      241 ggaagaacgg taccgcttcg gcgtacttgt gcccttcgta cagctcaacg ccgcggttga
      301 aggccagctc ctggtatact tcctgcaagc ggggatcctt gtcgcggatg gcgtccagag
      361 cctgcaaggc cgcctcgtag ttcctcgttt tcaggtacac gttcatcagg aactgctgcg
      421 cctcgtcgtg gcggccggaa ttgggatagg ctttcaggta gtcgcgcagg gcgatgatgg
      481 cctcgttgta cggatcgaag ctcagctcgt acgcgagctt ggcgtagttg aagagcgcat
      541 cctcggtgac gcgctggtcc acaccgatgt cgtaggcccg cttgaaggcg ttgcgtgcgt
      601 agttcttctc gttcaggctg aggtagcagt cggccatgtg gtacgccgcc aactgggcca
      661 ggctgtcctc caccggctgg ttcccgggac tggccaccag gttgaactgt gccagggctt
      721 tcttgcagtc gccgttcttg tagaagacgt agcccgcgat gtagcgatcg ccgcgctcca
      781 caccggtgcc ttgcacactc ttctccagat agggctgcgc ttccttgaac tggccggtcc
      841 ggtagtacgc ttcgcccgcc agccggttga tgtcaccctt cgacttcacg ctgccgccct
      901 cggccagcag tggcttcgtg tattcgagga gcgcattgta atcgccctgc aggaatttga
      961 tctgcgcgat gtagtacggc accgttctgg cgaaagcctc gtcgctctgc agcttctcga
     1021 atccttccag cgcggccgtg tagttcttgc gcgtatagtt gatgtgggcg gtgtagtagg
     1081 tggccgatgg tgcgtaggcg ctgctgccat ccttcacctt gctgaactcg gccagggccg
     1141 gatccacctg ctcctcctgg aagtacgcat agcccctttt gaaatggaac tcgtcgcggc
     1201 cgctctgcga aagcccgagg tcgtccacct tcatggcgaa ggcgagcgct tccttccact
     1261 ccttctggtt gaaccagttg cggaacaatt cgaggttgac ggccccggta cggaagctct
     1321 ccggatgctc ctccaggaag gcttgcagcc gctgtccggc atcgtcgttc gagaggcgta
     1381 cggcacagat ggcgctgtag aactcggctt cggtgcgcac cggatcgtgc ggatcgcgga
     1441 tgcggtcgcg cacccgttcc atctcg
//
LOCUS       DAOQOC010000915         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_72319_length_1465_cov_3.239716, whole genome shotgun sequence.
ACCESSION   DAOQOC010000915 DAOQOC010000000
VERSION     DAOQOC010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72319_length_1465_cov_3.239716"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PL106_13060"
     CDS             <1..1052
                     /locus_tag="PL106_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34048.1"
                     /translation="LGRIRAKVPQVMSAKYYRVIAPQFNPSGLPLDPHETVFLEADAE
                     CYQELRLFWILTRIANLGTVGRQATDSFAFYLAEDDIPFMLIVNHVGSDPDHPISGWS
                     IHIPRKLLWAEAMSDELLVFMLLHEMGHALQVPESELNADWWAMNIGLPGFYGQERAA
                     SLRDGLADSFKEYCRSVYTSSTYAHSSCTRDSVDFYPNLECRLRGIRGPDWIGEDGDR
                     IQSYTDLDECWSQGIMCGPKGDNKNVVGTCCTDKPCPECPPSRSTNNVLPGVVRITDI
                     LDSTLDSLSFISHLGKLGICSIKPELCAMSDTQRRTWLSDNIPSFDRRERRIEKLLME
                     SVIRTRYLNERILSK"
     gene            1199..>1465
                     /locus_tag="PL106_13065"
     CDS             1199..>1465
                     /locus_tag="PL106_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34049.1"
                     /translation="MSAELARSTNSRDRDLGILALSAGLFRKDDLLGCTRALDSLDRS
                     LPSADPFLRATGMRIRSMLMTQVGSPSKGVGAAEAGLAIVRGKGW"
BASE COUNT          313 a          420 c          408 g          324 t
ORIGIN      
        1 acctgggccg tatccgcgca aaagtgcctc aagtcatgtc agccaagtac taccgcgtca
       61 tagctccgca attcaaccct tccggccttc cgctcgatcc gcatgaaacc gtatttctcg
      121 aagccgatgc tgagtgttac caggaactcc ggctcttctg gatactgacg cgtatcgcca
      181 acctcgggac cgttggacgg caggcgacgg actctttcgc tttctatctc gcggaggacg
      241 acatcccctt catgttgatc gtgaaccatg tcggatccga tcctgaccac ccgatcagtg
      301 gctggtccat ccacatcccg agaaaactgc tgtgggctga agccatgagc gatgagttgc
      361 tcgttttcat gttgctccat gaaatgggcc atgcgctcca ggtgcccgaa tcggagctta
      421 atgcggattg gtgggccatg aacatcgggc tgcccggatt ctacggtcag gagcgagcgg
      481 ccagcttgcg cgatggcttg gcagactcct tcaaagaata ttgcaggtct gtttatacaa
      541 gttcaaccta cgcacattcg agttgcacaa gggactccgt ggacttctac cccaacttgg
      601 aatgccgctt gcgcgggata cgcggccccg actggatcgg ggaggacggt gataggatac
      661 agtcctacac ggatctcgat gaatgttggt cacaaggtat catgtgcggt ccaaaaggtg
      721 acaacaagaa tgtggtagga acctgctgca ccgacaaacc gtgccctgaa tgcccaccct
      781 cccgctccac gaacaatgtg ttgcctgggg tggtccgcat aacggatata ctcgacagta
      841 cccttgattc gctgtccttc atttcacacc tggggaagtt gggcatatgt tcgataaaac
      901 cggagttgtg cgccatgagc gacactcaac gacgaacctg gctgtccgat aacatcccat
      961 cgttcgatcg gcgcgagcgg aggatcgaga aactcttgat ggagagcgtg atccgcacga
     1021 gatacctcaa cgaacggatc ctgagcaagt gaagacctgt tgggtcggat ccatttggct
     1081 tgcggcatcc atcgggatgt tttcgagcct accgttgcgg tcccaacacc cggattggcc
     1141 ggcgtacagt tcagttagcg cggcttttga ccggaatgaa cccggtatcc tgctggactt
     1201 gagcgccgaa ctggcccgtt caacgaacag cagggatcgt gatctcggca tccttgccct
     1261 gtccgccggg ttattcagaa aagacgacct cttaggctgc actcgggcac tggacagcct
     1321 tgaccggtcg ctaccatcgg ccgatccctt ccttcgcgcc accggcatgc gcatccgatc
     1381 catgctgatg acgcaggttg gcagtccttc caagggggtc ggtgcagctg aagcaggctt
     1441 agcaatcgtt cgtggtaaag gctgg
//
LOCUS       DAOQOC010000916         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_72867_length_1460_cov_3.044128, whole genome shotgun sequence.
ACCESSION   DAOQOC010000916 DAOQOC010000000
VERSION     DAOQOC010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72867_length_1460_cov_3.044128"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PL106_13070"
     CDS             complement(<1..634)
                     /locus_tag="PL106_13070"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNA34050.1"
                     /translation="MSIAPALEERIARFVPITLAEMDRVKLQARVDTKYVFGEHDMPA
                     ILDTLMTEYRLLELNGVRGANYTSIYLDTPERAHYLAHHNGRTFRHKVRFRSYVGSDL
                     VFLEVKRKTGRGDTDKVRIRVNAIGDGLTPEQAAFVARTTRRNEALEPVLRNDFHRLT
                     FVHRTRQERLTIDGGLCYSAGDRRVELTGIRIAELKSSGSALGSPFTALMR"
     gene            763..1269
                     /gene="cdd"
                     /locus_tag="PL106_13075"
     CDS             763..1269
                     /gene="cdd"
                     /locus_tag="PL106_13075"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNA34051.1"
                     /translation="MVNHRKIALHYSHHSSWKSLPKEDRELLELAAKVARNAYAPYSK
                     FRVGAALRMDDGSTVTGSNQENASFPAGTCAERTALHAAMTAKPKGAVESMAIVVPQV
                     RGQKPVTPCGICRQALVEQERRQEGPIRLLMGITRGPVIEMFTVDALLPMSFDSGHLP
                     GARRKKRS"
BASE COUNT          270 a          447 c          483 g          260 t
ORIGIN      
        1 agcgcatcag ggcggtgaag ggcgagccga gcgcactgcc cgaactcttg agttcggcga
       61 tgcggatgcc ggtgagttcc acccggcggt caccggccga gtagcacagg ccaccatcaa
      121 tggtaaggcg ttcctgccgc gtgcgatgga cgaaggtgag gcggtggaaa tcgttgcgga
      181 gtacgggctc cagggcttcg ttcctgcggg tggtccgcgc cacgaaagcc gcctgttccg
      241 gggtgagccc gtcgccgatc gcgttcaccc ggatgcgcac tttgtcggta tcgccccggc
      301 cggtcttgcg cttcacttcc aagaagacca ggtcgctgcc cacgtacgac cggaaacgga
      361 ccttgtggcg gaaggtccgt ccgttgtgat gggccaggta gtgcgcccgc tccggtgtat
      421 ccaggtagat ggaggtatag ttcgcgccgc gcaccccatt caattcaagc agacggtatt
      481 cggtcatcaa cgtgtccagg atcgccggca tgtcatgctc gccaaagacg tacttggtgt
      541 ccacccgggc ttgcagcttc acccggtcca tctcggccaa ggtgatcggt acgaagcggg
      601 cgatgcgttc ctccaatgcg ggggcgatgc tcatgacaag gtggcgaatt taggcgggtg
      661 gcgcacgcga ccacagcttg ggagcgtggc gctcctttga tcgtcggcat gcggtgggtt
      721 cgcagcgcgt ccgcagtaca ttcggcgacc tttacgagca ggatggtcaa tcaccgcaag
      781 atcgcgctcc attactcgca tcattcgtcg tggaagtcgc tccccaagga ggaccgggaa
      841 ttgcttgaac tcgcagcgaa agtggcccgg aacgcttacg cgccctattc caagttccgg
      901 gtgggcgcgg cactccggat ggacgatggg agtacggtga ccggcagcaa ccaagagaac
      961 gcatcattcc cggcgggtac ctgtgccgag cgcacagcct tgcatgcggc catgacagcg
     1021 aagccgaagg gggccgtgga aagcatggcg atcgtggtgc cccaggtgcg cggccagaaa
     1081 ccggtgacgc cgtgcgggat ctgtcgccag gccttggtgg aacaggagcg caggcaggag
     1141 gggccgatcc gcctgctcat gggcatcacg cgcggtccgg tgatcgagat gttcacggtg
     1201 gacgccctgc tgcccatgtc cttcgacagt gggcacttgc ccggggcgcg ccggaagaag
     1261 cgttcgtaag gccaggacct acggagcccc atccggggta cttttggccc atacgcgatg
     1321 agcaacccta cgaccgcaac gaagaaaaag gccgcggcac cacctgccct cggcaaggag
     1381 acccacctgc gctggttcaa ggacatgatc ctgatgcgcc gctttgaccc cgtccgcctc
     1441 atcatcatcg ccatgctgtc
//
LOCUS       DAOQOC010000917         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_72897_length_1460_cov_2.474021, whole genome shotgun sequence.
ACCESSION   DAOQOC010000917 DAOQOC010000000
VERSION     DAOQOC010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72897_length_1460_cov_2.474021"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PL106_13080"
     CDS             <1..554
                     /locus_tag="PL106_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNA34052.1"
                     /translation="AGMNLGEGAAYLILEREDDAARAGRTPLALLAGWCNSNDAHHQT
                     ATSPDGAGPYRAMAGALRMAGATANDVDHINAHGTGTDNNDGTELAAMERLFPVMPPF
                     TSTKGSTGHTLGAAGALEAVIAIICMREGIIPASPNPIVPMAGHRSGPVTTTLRRPVR
                     NVLSSSFGFGGNDTALYLTSPRP"
     gene            551..1393
                     /locus_tag="PL106_13085"
     CDS             551..1393
                     /locus_tag="PL106_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNA34053.1"
                     /translation="MIVRATSRSGITDGAITFNMPAGADRRMARIQRAACAAAYGAMS
                     KAGITRVDGIFSATTLGCLEDTERFLAEMGAQQGSLLSPLPFMRSTHNTMAGQLALLL
                     KAQGPNLTFSQELFGLHAALIHARLHLREHPGDAVLAFAADEHTPLLSWIAGQLGSEA
                     IPGEDAEAFVLRANGGAGIARIERILLGEAKAGAAWWTDLCGSIPGSIDHTIVVNDLP
                     GGDGAPRTLDLDEAPMDGPMGTRTARVLAWVLGRMQEGAINGRVLLADRGTDQQGGIL
                     VAPC"
BASE COUNT          255 a          476 c          492 g          237 t
ORIGIN      
        1 gtgcgggcat gaacctgggc gagggcgcgg cctatttgat ccttgaacgc gaggatgatg
       61 cggcacgggc gggcagaaca ccgctggcgt tgctggccgg atggtgcaac agcaacgacg
      121 cgcaccacca gacggccact tcgcccgatg gcgcgggacc gtaccgggcc atggccggcg
      181 cgctgcgaat ggcgggtgcc acggcgaacg atgtggacca catcaacgcg cacggtaccg
      241 gcacggacaa caacgatggg accgagctgg cggccatgga gcggctcttc ccggtgatgc
      301 cgcccttcac cagtaccaaa ggatccaccg gtcataccct gggcgccgcc ggggcgttgg
      361 aggcggtgat cgcgatcatc tgcatgcggg agggtatcat tccggcatcg cccaacccga
      421 tcgtgccgat ggcaggacac cgctccgggc cggtcaccac caccttgcga cggccggtgc
      481 gcaatgtgct gtccagttcc ttcgggttcg gcggcaacga cacggcgttg tacctcacct
      541 cccctcggcc atgatcgtca gggccacctc acggagcggg atcacggatg gtgcgatcac
      601 cttcaacatg cccgcagggg ctgaccggcg catggcacgc atccaacgtg cggcatgcgc
      661 ggccgcgtac ggcgccatgt ccaaggcggg catcacccgg gtagatggta ttttttccgc
      721 tacgacactg ggctgcctgg aggacaccga acgcttcctg gcggagatgg gggcccagca
      781 gggatcgctg ctctcgcccc tgcccttcat gcgctccacc cacaacacca tggccgggca
      841 gctggccttg ctgttgaagg cacaggggcc gaatctcacc ttctcgcagg agttgttcgg
      901 cttgcatgcc gccctgatcc acgcccgtct gcacctgcgg gaacatcccg gcgatgccgt
      961 gctggccttc gccgccgacg agcacacgcc actgctgtcc tggatcgcgg gacaactcgg
     1021 gagcgaggcc atccccgggg aggatgccga ggccttcgta ctgagggcga acggtggggc
     1081 cgggatcgcc cggatagaac ggatcctgct cggtgaagcg aaggcgggcg cggcatggtg
     1141 gaccgacctc tgtggatcga tacccggaag tattgatcac accatcgtgg tgaacgacct
     1201 gccgggaggt gatggcgcac cccgtacact ggaccttgac gaagccccga tggacggccc
     1261 catgggcacg cgtaccgcgc gggtgctggc gtgggtgctg gggcgcatgc aggaaggcgc
     1321 gataaacggc cgtgtgttgc tggccgatcg cggtacggac caacaggggg gcatcctcgt
     1381 agccccatgc tgacccaccg ccgcacgtgg ccgctcttcg ccatcgcaaa tttaaagtgg
     1441 tgatcgatta ttctcactcg
//
LOCUS       DAOQOC010000918         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73010_length_1459_cov_1.844017, whole genome shotgun sequence.
ACCESSION   DAOQOC010000918 DAOQOC010000000
VERSION     DAOQOC010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73010_length_1459_cov_1.844017"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..793)
                     /locus_tag="PL106_13090"
     CDS             complement(155..793)
                     /locus_tag="PL106_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34054.1"
                     /translation="MLQKCRLPHLITAVLCLEVAIYLWAVWTSTLDSGNFFAITPEFI
                     FDKCARNAGRVSSGINLLILLLVGHKGLSAIFRNGSSRIELLVLITLFSINHLIHLFF
                     VIQNFAHHGMDLSIGENLHGFLTFFLIVIVPVVLWMSHEFGPALYVLIIFHLLNISYF
                     TMETFLSKVKPGKPAYHEQLGMAITLLACVYVMHSVVRDYKTHLRAVGVPRR"
     gene            complement(881..>1459)
                     /locus_tag="PL106_13095"
     CDS             complement(881..>1459)
                     /locus_tag="PL106_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNA34055.1"
                     /translation="VPSGRRRMAPSVPNLRPMPNLEHLPALLPNGHVQAVIEIPAGTS
                     DKRQYDIATNTFPVDLRNGIPRRIKFLPYPANYGFIPGTRMDKAQGGDGDALDIFVLC
                     AGVASGTVLEVEPIGIIELLDAGERDDKVIALPVDPALRTVEASDILGLPQAARDILV
                     AWLLNYDPEDGAKLVGVRDKAEAMAAIRKWVE"
BASE COUNT          313 a          416 c          450 g          280 t
ORIGIN      
        1 atgggccgca tccgccaggc actggaaaag gaaacagggg cggagttgcg ggggtgagcg
       61 ggcgtggtgt tggcgccggt cgttgccacc gcgccgactt cttgaaatgt tcaggaccgc
      121 tgcccgtgac cggggtctgg aatgctgacc gggatcaccg tcttggaacc ccaacggcac
      181 gaagatgggt tttgtagtca cggaccacac tgtgcatcac atagacgcac gcaaggagag
      241 tgatcgccat gcccagctgc tcgtggtatg cgggctttcc cggctttacc ttggagagga
      301 aggtctccat ggtgaagtag ctgatgttga gcagatgaaa gatgataagt acgtagagcg
      361 ctggaccgaa ctcgtgggac atccacagaa ccaccgggac gatcacgatc aaaaagaatg
      421 tcaggaagcc atgtagattc tccccaatgg aaagatccat cccatgatgg gcgaaattct
      481 gaatgacaaa gaacagatgg atcaaatggt tgatggagaa cagcgttatg aggaccaaca
      541 actcgattct tgagctgcca ttccgaaaga tcgcggacaa gcccttgtga ccgaccaaca
      601 agaggatcaa tagattgatg cctgaggaca cacggcctgc gttgcgagca catttgtcaa
      661 agatgaactc cggggttatc gcgaagaaat tcccgctgtc cagcgttgac gtccataccg
      721 cccagaggta gatcgcgact tccaagcaga gcacggccgt gatcaagtgc ggtagccggc
      781 atttctgcaa catgatgatt ggcattgacg cacaatacca acgaaacggg ctgccgtgta
      841 ttgccctaac gcgtcctgct ggatccgagc cctgccaggc ttattccacc catttcctga
      901 tcgcggccat ggcctcggcc ttgtccctca cgcccaccag cttggccccg tcctccgggt
      961 cgtagttcaa cagccaggcc accaggatgt cgcgtgcggc ttgcggcagg ccgaggatgt
     1021 cgctggcctc cacggtgcgc agggccggat ccacgggcag ggcgatcacc ttgtcgtccc
     1081 gttcgcccgc gtccaacagc tcgatgatgc cgatgggctc cacctccagc acggtgccac
     1141 tggccacgcc ggcgcaaagc acgaagatgt ccagcgcgtc cccatcgcca ccttgggcct
     1201 tgtccatccg ggtgccgggt atgaaaccgt agttggcggg atacggcagg aacttgatgc
     1261 ggcgcggtat gccgttccgc aggtcgaccg gaaaggtgtt cgtggcgata tcgtactggc
     1321 gcttgtcgct ggtaccagct ggtatctcga tcacggcttg cacgtgaccg ttgggcagca
     1381 gggcaggtag gtgttccaag ttgggcatgg ggcgcaagtt cggcacgctg ggtgccatgc
     1441 gccgccgccc ggacggcac
//
LOCUS       DAOQOC010000919         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73463_length_1454_cov_2.760543, whole genome shotgun sequence.
ACCESSION   DAOQOC010000919 DAOQOC010000000
VERSION     DAOQOC010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73463_length_1454_cov_2.760543"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /gene="mnmG"
                     /locus_tag="PL106_13100"
     CDS             complement(<1..1386)
                     /gene="mnmG"
                     /locus_tag="PL106_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNA34056.1"
                     /translation="MGLHEQYDLVVVGAGHAGCEAAAAAANLGSRVLLVTMNMGVIGQ
                     MSCNPAMGGVAKGQIVREIDALGGYSGIVSDRSMVQFRMLNRSKGPAMWSPRTQNDRD
                     LFAREWRRMLERTPNVHFLQDTVVRVEVDDRTNEVRGVITGIGVRIPCRAVVLTSGTF
                     MNGVMHIGEKQFGGGRAGEKASHGLTEQLVELGFEAGRMKTGTPPRVDGRSIDYTLLE
                     EQPGDEQPGKFSYLPSTAPPLRQLSCWITWTNADVHDTLRSGFDRSPLFNGRIRGRGP
                     RYCPSIEDKIDRFAEKDRHQIFVEPEGWDTVETYINGFSTSLPEEVQLAALRLLPGFQ
                     HARMFRPGYAVEYDYFPPTQLRPTMETKLVSGLYFAGQINGTTGYEEAACQGLMAGIN
                     AHQQLTDKAPFILGRDEAYIGVLIDDLITKGTEEPYRMFTSRAEFRILLRQDNADLRL
                     TERSHNIGLATD"
BASE COUNT          237 a          457 c          459 g          301 t
ORIGIN      
        1 atcggtggcc aggcctatgt tgtgcgaccg ttccgtcaac cgcaggtccg cattgtcttg
       61 acggagcagg atgcggaatt ccgctcggct cgtgaacatg cgataaggct cctcggttcc
      121 cttggtgatc aggtcgtcga taaggacccc gatataggcc tcatcacgac ccaggatgaa
      181 cggggcttta tcagttagct gctggtgcgc gttgatcccg gccatcaagc cctggcaggc
      241 ggcctcttcg tatcctgtag tgccattgat ctgcccggcg aaatagagcc cggaaaccaa
      301 cttggtctcc atggttggcc tgagctgggt aggagggaag tagtcgtatt ccaccgcata
      361 tccggggcgg aacatgcgtg cgtgctggaa tccggggagc aggcgcaggg ccgccagttg
      421 cacctcttcg ggcaggcttg tgctgaatcc gttgatatag gtctcgacgg tgtcccagcc
      481 ctcaggctcc acgaagatct ggtgacgatc cttctcggca aagcgatcga tcttgtcctc
      541 gatgctgggg cagtaccgcg gtccgcgtcc tcggatccgc ccgttgaata ggggactgcg
      601 gtcgaagccg ctgcgaagcg tgtcatgcac atcggcattg gtccacgtga tccagcagct
      661 gagttgtcgc aggggtggtg ctgtgctggg caagtagctg aacttgcccg gctgctcgtc
      721 ccctggctgt tcttccagca aggtgtagtc gatactgcgg ccatccacgc ggggtggcgt
      781 gccggtcttc atgcggcctg cctcaaagcc cagttccacc agttgttcag tcaggccgtg
      841 gctggctttc tccccggccc ggccaccccc gaactgtttc tccccgatgt gcatcacccc
      901 gttcatgaag gtgccgctgg tgagcactac agcccggcac ggtatgcgga caccgatccc
      961 tgtgatcaca ccgcgcactt cgttggttcg atcgtccacc tccacgcgca ccacggtgtc
     1021 ctgcaggaaa tgaacgttgg gtgtgcgctc caacatgcgg cgccattcgc gggcgaagag
     1081 gtcgcggtcg ttctgggtgc gcgggctcca catggcggga cccttgctgc ggttgagcat
     1141 gcggaactgc accatgctcc gatcgctcac aatgccgctg tagcccccga gggcatcgat
     1201 ctcgcgcacg atctggccct tggccactcc ccccatggcc gggttgcagc tcatctggcc
     1261 gatgaccccc atgttcatgg tgacgagcag cacgcgactg cccaggttgg ccgcagccgc
     1321 ggccgcctcg cagcctgcgt gcccggcacc gacaacgacc aggtcgtatt gctcgtgaag
     1381 gcccatcggt gatccgcgcc gcgcgccggg caaaggtact gggggtcggg ggctgtcgca
     1441 tgaggtgttc acag
//
LOCUS       DAOQOC010000920         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73472_length_1454_cov_2.596855, whole genome shotgun sequence.
ACCESSION   DAOQOC010000920 DAOQOC010000000
VERSION     DAOQOC010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73472_length_1454_cov_2.596855"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PL106_13105"
     CDS             <1..502
                     /locus_tag="PL106_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34057.1"
                     /translation="DGYGMLELPWGTVPGVLAIRELDTTYMSPGNDTVPHVRWMRRFV
                     RPGWPVDIAHIWTFAPGDSLGYSGPHGGGYLSEEVFQAIAEAEEGTSLSLSWDPVTDR
                     LIVALPPGTQGEVFIVELSGRVVFQSGWWGPLIATDHLASGIYVVRFFDHTSRRVRAE
                     RFVKYR"
     gene            499..1389
                     /locus_tag="PL106_13110"
     CDS             499..1389
                     /locus_tag="PL106_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34058.1"
                     /translation="MMMESDRDTPSNIRHPVLQWLIYGHLWLVLAVVAQILWTGLFLH
                     EAPELWRYTTAAALGTFAGYATMRLARVNSPEAGRYLNLIWYKEQRKLITALVGVAGS
                     AAFLLMWPLWRSLWHWLLPVVAMALLYLTPFTSGTGRGFGLRSIPFLKVLLIPGLWVI
                     VCSAVPMRLDLFDHAPGAILAFACMRLPLVMALTVVFDIRDSLTDDPALRTMPQVFGI
                     RGAKVIASVLLLCSALYEVIFLRTLGYPNAVWSLLVAYVVALGLTLRARPVRDPIYYA
                     LYVDGVMILIPLCAWIGTLV"
BASE COUNT          212 a          463 c          465 g          314 t
ORIGIN      
        1 ggatggctac ggaatgctgg aactgccgtg gggaacggtt cccggcgtgc tcgccatccg
       61 ggaactcgat acgacgtaca tgtcaccagg taacgatacg gtcccacatg tgcgctggat
      121 gaggcgtttt gtacggcccg gctggccggt ggacatcgcg cacatctgga ccttcgcacc
      181 gggcgacagc ctgggatatt cgggccccca tggcgggggc tacctgtccg aggaagtgtt
      241 ccaagcgatt gccgaggcgg aggaggggac gagcctgtcc ctgtcatggg atccggtcac
      301 ggaccggttg atcgtggcgc tgccgcccgg gacccagggt gaggtgttta ttgttgagct
      361 atctggccga gtggtgttcc aaagcggatg gtggggtccg ttgatcgcaa cggaccacct
      421 cgccagcggc atttatgtgg tgcgcttctt cgatcacacc tcacggcgag tccgggcaga
      481 acgattcgtg aaataccgat gatgatggag tcggaccggg acaccccgtc gaacatccgc
      541 cacccggtac tccagtggct gatctacggt cacctctggt tggtgctggc cgtggtggcc
      601 cagatcctgt ggacaggcct cttcctgcac gaggcgccgg agctgtggcg ctacaccacg
      661 gccgccgccc tcggcacctt cgcgggctat gccaccatgc gcctggcacg cgtgaacagc
      721 cccgaggccg gtcgctacct gaacttgatc tggtacaagg agcagcgcaa gctcatcacc
      781 gcgctcgtgg gcgttgccgg gtcggccgcg ttcctcctga tgtggccgct gtggcgctca
      841 ctctggcatt ggctgctgcc cgtggtggcc atggcgctcc tgtacctcac gcctttcaca
      901 tcgggcacag gtcggggctt cggcctgcga tccattccct tcctgaaggt gctgttgatc
      961 cccggccttt gggtgatcgt gtgcagtgcc gtaccgatgc gtttggatct tttcgatcac
     1021 gccccggggg ccatcctcgc cttcgcgtgc atgcgactgc cactggtcat ggcgctcacc
     1081 gtggtcttcg acatccgcga ttcactcacc gacgaccctg cgctacgcac catgccccag
     1141 gtcttcggca tacgcggtgc aaaggtcatc gcatccgtcc tgctcctgtg ttccgcactg
     1201 tacgaggtga tcttcctgcg caccttgggc tatccgaacg ccgtatggag cctgttggtg
     1261 gcctacgttg tggcgctcgg gctcaccctg cgtgccaggc ccgtgcgcga tccgatctac
     1321 tatgccctgt acgtggacgg ggtgatgatc ctgatccccc tgtgcgcgtg gatcggcacg
     1381 ttggtgtgag tggtgcaccg gtgccagcgt cccttgttcc acgtgtggca ccgcgtccac
     1441 cggcctgcta cttt
//
LOCUS       DAOQOC010000921         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73529_length_1453_cov_3.751073, whole genome shotgun sequence.
ACCESSION   DAOQOC010000921 DAOQOC010000000
VERSION     DAOQOC010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73529_length_1453_cov_3.751073"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1453
                     /locus_tag="PL106_13115"
     CDS             129..>1453
                     /locus_tag="PL106_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNA34059.1"
                     /translation="MASRRSRPTDDAKPAKLTKVALRKFFRVFRYMRGQRGLFAVGMV
                     CLLLTSLLSAAFPFLIGKLIDARAGTGFWQAPVNDLGNVHSILKLLLLVFAAQAFFGF
                     WRIWTFGSVTENALTALRRDTYAHVLKLPMAFFAQRRVGELNSRISADVTLLQDTFTT
                     VLAEFLRQFVVISVCIVALTYLSSELMITMLATLPVVMLVAVFFGRYIRKLSKQVQDR
                     IAESNVIVEETLQGIQSVKAFSNEGHETARYGASVMAARALALKSLRWRGGFVSFIIF
                     CMFGVVVFVVYRAALLKDAGLMSVGDITSFVAFSIMIGASIAGLPEQVTSLLKAVGAT
                     ERLMDLHDEAAEAVSLGPKPAPLLLQGRIAFDRVAFHYASRPDVPVLRDVSFAAEPGE
                     RIALVGPSGAGKSTLASLVLRFYDPEEGAVLIDGKDARAYDLTALRDRMA"
BASE COUNT          247 a          462 c          465 g          279 t
ORIGIN      
        1 cacctgtgac ggcagaccag ttccaaccgg aggtattcgg cgtgaccttg ccgagcaacg
       61 acaatttttg tgattgaacc aacggtggct agaataagca tcctgcacta acccccacct
      121 ttggggccat ggcctcccgc cgctcccgcc ccaccgacga cgcgaaaccg gcaaagctga
      181 ccaaggtcgc cctgcgcaag ttcttccggg tgttccgcta catgcgcggg cagcgcgggc
      241 tctttgccgt gggcatggtg tgcctgctgc tcaccagcct gttgagtgcg gccttcccct
      301 tcctcatcgg caagctgatc gacgccaggg ccggcacggg gttctggcag gcaccagtga
      361 acgacctggg caatgtgcac agcatcctca agctgctgct gctggtcttc gccgcgcagg
      421 ccttcttcgg cttctggcgc atctggacat tcggttccgt taccgagaac gcactgaccg
      481 ctctgcgccg cgatacctac gcgcacgtat tgaagctgcc catggccttc ttcgcgcagc
      541 gccgggtggg tgaattgaac agccgcatca gtgccgatgt caccttgttg caggacacgt
      601 tcaccacggt actggccgaa ttcctgcggc aattcgtggt gatcagcgtg tgcatcgtgg
      661 cgctcaccta cctgagcagc gagctgatga tcaccatgct ggccaccctg ccggtggtga
      721 tgctggtagc ggtcttcttc gggcgctaca tccgcaagct gagcaagcag gtgcaggacc
      781 gcatcgccga gagcaacgtg atcgtggagg agaccttgca gggcatccag agcgtgaagg
      841 ccttcagcaa cgagggacac gagaccgcgc ggtacggcgc gagcgtgatg gccgctcggg
      901 cactggcctt gaagagcctg cgttggcggg gcggcttcgt cagcttcatc atcttctgca
      961 tgttcggcgt ggtggtcttc gtggtgtacc gcgcggccct gctgaaggac gccggtctga
     1021 tgagcgtggg cgacatcacc agcttcgtgg ccttcagcat catgatcggc gccagtatcg
     1081 caggcctacc cgaacaggtc acctccttgt tgaaggccgt gggggccacc gaacggttga
     1141 tggacctgca cgacgaagcg gccgaagcgg tgagcctggg tccgaagccg gcaccactcc
     1201 tgttgcaggg ccgcatcgcg ttcgatcggg tggccttcca ctacgcttcg cggcccgatg
     1261 tacccgttct acgggatgta tcgttcgcgg ccgaacccgg ggaacgcatc gccctggtgg
     1321 ggcccagcgg cgcgggcaag agcaccctgg cctcgctggt actgcgcttc tacgatccgg
     1381 aggaaggtgc ggtgctcatc gacgggaagg acgcgcgcgc atacgacctg acggccttgc
     1441 gcgaccgcat ggc
//
LOCUS       DAOQOC010000922         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73572_length_1453_cov_2.665951, whole genome shotgun sequence.
ACCESSION   DAOQOC010000922 DAOQOC010000000
VERSION     DAOQOC010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73572_length_1453_cov_2.665951"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1386
                     /locus_tag="PL106_13120"
     CDS             151..1386
                     /locus_tag="PL106_13120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA34060.1"
                     /translation="MSGLLRPFLWGAVPLSTLLLAVMPVLPTRAMVLVIVAWLVLIVL
                     RAFVDPVPITRAQLRWCGFLALPFLLMLLDNLRAPDLAEGWKHTERSAALLLFPVGFL
                     LLGAPSTAPFRRAMTDVFSAAAMALALYANIGITISGIPASTGGGFAYDCRAAFTAIT
                     SLHPPYAAYFLLCAALFQLDRVLCGATYRAWRIAAILLLFTSSALLASRMPLVAFLVA
                     ALCIVALRLHRGRALVLAGTILLGGVLTALALPGARQRIMESFDATAIPATQAEVNSS
                     NIRRAIAHCTHEAIGAHWLVGTGQARAQATLDTCYQQFGIPILLDGSYGTHNQPLHWW
                     LCFGIAGLLLFVLYFSVLLRSAWHVRDHAHFAFLVLLLLCMLTENLLARQWGVVLFAS
                     FNALFVAGRLETNTRPGST"
BASE COUNT          203 a          545 c          415 g          290 t
ORIGIN      
        1 ccatgaggaa cgaacccggg caccggatcc tgtgcgactt cgcgcctcgg tcgccccctg
       61 tttcaacgat cggttcgcac tgcgtatcca cgccatcgtg aacgaggttc tgagcgagcg
      121 atcaatgccc agaaccactg ccgacgcgcc atgagcggcc tccttcgacc cttcctctgg
      181 ggtgccgtcc cactgtccac cctgctgctc gccgtgatgc ctgtgctgcc aacgcgcgcc
      241 atggtcctgg tgatcgtggc ctggctggtg ctgatcgtac tccgggcctt cgtggatccc
      301 gtgcccatca cacgtgccca actccgctgg tgcggcttcc tcgcgctacc cttcctgctc
      361 atgctgctgg acaacctgcg tgcgcccgac ctggccgaag gctggaaaca taccgaacgc
      421 agcgccgccc tcctgctctt tcccgtgggc ttcctgctgc ttggcgcgcc atcaaccgcc
      481 cccttccgcc gcgcgatgac cgatgtgttc agcgcggcgg cgatggcact ggccctctac
      541 gcgaacatcg ggatcaccat cagcggcatc cccgccagca ccggcggcgg cttcgcgtac
      601 gactgccgcg cggccttcac tgcgatcacc tcgctccatc cgccctacgc cgcttacttc
      661 ctgctctgcg ccgcactctt ccagttggac cgcgtgctgt gtggagcaac ataccgtgcc
      721 tggcggatcg ccgcgatcct cctgctcttc acctcatcgg ccctgctggc ctcgcgtatg
      781 ccgctcgtcg ccttcctggt ggccgccctg tgcatcgtgg cactacgcct gcaccgtgga
      841 agggcgttgg tcctcgcggg caccatcctc ctcggcggcg tgctcaccgc gctggctctg
      901 cctggcgcac ggcagcgcat catggagtcc ttcgatgcca ccgcgatccc agccacgcaa
      961 gcggaggtca attcctccaa catccgcagg gccatcgccc attgcaccca cgaggcgatc
     1021 ggtgcgcact ggctcgtggg caccggccag gcccgggcac aggccacgct cgatacgtgc
     1081 taccagcagt tcgggatccc gatcctgctc gatgggagct acggcaccca caaccagccc
     1141 ctccattggt ggctctgctt cggtatcgcg gggctgctgc tcttcgttct ttacttcagc
     1201 gtgctgctgc ggtccgcctg gcatgtgcgc gatcacgccc acttcgcctt cctcgtcctc
     1261 ctgctgttgt gcatgctcac cgagaacctc ctggcacggc aatggggcgt ggtgctcttc
     1321 gccagcttca acgcgctgtt cgtggcggga cggttggaga cgaacacgcg accgggatcc
     1381 acgtagcact accgtttcac gaagcgtgcc atcgccaagc gcataccgtc gcgggccagc
     1441 atggtaacgg cat
//
LOCUS       DAOQOC010000923         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73715_length_1451_cov_5.105301, whole genome shotgun sequence.
ACCESSION   DAOQOC010000923 DAOQOC010000000
VERSION     DAOQOC010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73715_length_1451_cov_5.105301"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PL106_13125"
     CDS             complement(<1..205)
                     /locus_tag="PL106_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34061.1"
                     /translation="MRPVAVLLFIVFVLAVLALIGRIFPSDGIRIGAVTLEFPDALAR
                     MASPADKKVDISAIIALKVDSAGL"
     gene            complement(202..>1451)
                     /locus_tag="PL106_13130"
     CDS             complement(202..>1451)
                     /locus_tag="PL106_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA34062.1"
                     /translation="VPLDTALSGERLRGLTDGMSAFQDDAVADAILLFGGPRRSDTEL
                     MLGLATPWLPLIADELREAGLPDDLKYVPLALSAMNPCATNTHGEAGPWMLSYPVAVR
                     YGLVINDTIDQRHDMVRATTAAVRYMKHLYSSHPPDQALLAILCGPANLARAKIRSGG
                     AATLAALYPHADAAERDRVPAFMALRYLVHYANDLGIRPTRLDPPAIDTLRTGPSVRR
                     TVFTTLLGVDPRAFAELNPVIVGHHLPASTLIRVPQGAAGRYISMADSIAHASAPVID
                     TLPDAPILLNHTVRSGEYLGGIAEDHGVSVAELMEWNGLESDLLHEGMVLRVRVPATV
                     AGTLEHDRPDGTTAKDLSARSTTAPEGTHAGRPRVYTVRRGDTLSAIAKRYKGVSARD
                     IMRYNGIGETIRAGQRIKIPLPR"
BASE COUNT          263 a          463 c          474 g          251 t
ORIGIN      
        1 aaaggcctgc gctgtccacc ttcaaggcaa tgatcgctga gatgtccacc ttcttgtccg
       61 ccggcgaggc catccgggcg agggcgtccg ggaactccag cgtcacggcc ccgatccgga
      121 taccatcgct cgggaagatc ctgccgatga gggccagaac ggcgagcacg aacacaatga
      181 acagcaggac ggcgaccggc ctcatctggg gagggggatc ttgatccgtt ggccggcccg
      241 gatcgtttca ccgatgccgt tgtaccgcat gatgtcgcgc gcgctcacgc ccttgtaccg
      301 cttggcgatc gcactgagcg tgtcaccgcg tcgcaccgtg tacacacgag gccgaccggc
      361 atgcgtgcct tccggggcgg tggtgctgcg tgcggacagg tctttggccg tggtgccatc
      421 cggccggtcg tgctccaggg tgccggccac cgttgccggt acgcgaacac gcaacaccat
      481 gccttcatgc aagaggtcgc tttcaaggcc gttccactcc atcaattcgg caacgctcac
      541 accatgatcc tcggcgatgc ctccaagata ttcacctgaa cgaacagtat ggttcaaaag
      601 gatcggcgca tcgggaaggg tgtcgatcac gggggcggaa gcatgggcaa tgctgtccgc
      661 catgctgatg taccgcccag cggccccttg gggcacacgt atcaacgtac tggctggcag
      721 gtgatgtcca acgatcaccg gattcaattc ggcaaaggcc cgtggatcca caccgagcag
      781 cgtggtgaag accgtgcgtc ggaccgaagg gccggtgcgc agcgtatcga tcgcgggcgg
      841 atccaggcgc gtggggcgga tccccaggtc gttggcataa tgcacgaggt agcgcagggc
      901 catgaaggcg ggcacccggt cgcgctcagc ggcatccgcg tgcgggtaca gggccgccaa
      961 ggtggccgcg ccaccgctcc ggatcttcgc acgggccagg ttggccggtc cgcacaggat
     1021 ggccagcaag gcctggtccg gagggtgtga gctgtacaga tgcttcatgt agcggaccgc
     1081 ggccgttgtg gcccgcacca tgtcgtgccg ttgatcgatg gtatcattta tgacaagccc
     1141 gtaccgcacg gccactggat agctcagcat ccagggcccc gcctccccat gcgtattcgt
     1201 ggcgcacggg ttcatggcac tcagggccag tggtacgtat ttgaggtcgt cgggcaagcc
     1261 cgcttcacgc agctcatccg cgatcagggg caaccatggg gtggccaggc ccagcatcaa
     1321 ttcggtgtcg ctgcggcgcg gcccgccgaa caacaggatc gcatcggcca ccgcgtcgtc
     1381 ctgaaaggcg gacatcccat ctgtaagtcc tcgcagccgt tcgccactga gagcggtgtc
     1441 cagcggcacc c
//
LOCUS       DAOQOC010000924         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_73881_length_1450_cov_2.639427, whole genome shotgun sequence.
ACCESSION   DAOQOC010000924 DAOQOC010000000
VERSION     DAOQOC010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73881_length_1450_cov_2.639427"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PL106_13135"
     CDS             complement(<1..570)
                     /locus_tag="PL106_13135"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNA34063.1"
                     /translation="MRHLLFLLAVLPLATPARAQASGQVAPAAIGWYNVENLYDTIDS
                     PDTDDAEWLPTAPKNWTGERYRIKLAHLARVIGEMGQKIHPDGLACIGLCEIENATVL
                     EDLVHTPPIAARGYQYVHHDGPDRRGVDVAFLYDPKRFHLLNHTTHPLHDPQDSLFRT
                     REQLVVTGVMDGDTISCIMAHWPSRRGGEK"
     gene            complement(757..>1450)
                     /locus_tag="PL106_13140"
     CDS             complement(757..>1450)
                     /locus_tag="PL106_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34064.1"
                     /translation="DGPDPFRGMNRIILLVISTTIPTICGAQWDSIDYAPIIRDTSLV
                     VYDSGFTFREGVYFGFEDFRDNRPGVALKDLLDEEKRPVDDLRQSDGKLYHTDSSGTA
                     RPIDMDRIWGFCERNTVYVRIGNGFNRIGLMGSIAHIVYDQTYSSWGGYYGMYGMGPT
                     TTTVQEQRLLDMASGDFLPVNATGIAQAISTDPELLAKYEALPRKERNATETVYRFMR
                     LFNERHELRFPR"
BASE COUNT          249 a          414 c          497 g          290 t
ORIGIN      
        1 cttttcgcca ccacgccggc tgggccagtg ggccatgatg cagctgatgg tgtcgccatc
       61 catcacgccc gtcaccacga gttgttcacg ggtgcggaag aggctgtcct gtggatcgtg
      121 caatggatgc gtggtgtggt tcagcaggtg gaatcgcttc gggtcgtaca ggaaggcgac
      181 gtccacaccg cgccggtccg gaccgtcatg atgcacgtac tgataaccgc gcgcggcgat
      241 gggcggggtg tgaaccaggt cctccagcac ggtggcgttc tcgatctcgc acaggccgat
      301 acatgccagt ccatcgggat gtattttttg ccccatttcg ccgatcacgc gtgccaggtg
      361 cgccagcttg atgcggtagc gctcgccggt ccagttcttg ggtgccgtgg gcagccactc
      421 ggcatcgtcg gtgtccgggg agtcgatggt gtcgtacagg ttctccacgt tgtaccagcc
      481 gatagcggcc ggagccacct ggccggaggc ttgcgcacgt gcgggcgttg cgagaggaag
      541 gacagcaagg agaaagagga ggtggcgcat ggtggggtgg tgcgatcctg gacgttgtgg
      601 gaccgatggg atccgcttgc acagcggcgg cgaaagtagg tgccgtggtc cggcctgcac
      661 gaaccgggcc gaacggagga atcgcggaag ttcctgagcg gtgggcttcc gatccagcgg
      721 tcccgtggtt cagggatgac ccggatggag ccacgcttat cgcgggaaac gcagctcatg
      781 ccgttcgttg aacaggcgca tgaatcggta cacggtctcc gtagcgttgc gctccttcct
      841 cgggagcgct tcgtacttgg ccaacaactc gggatcggtg gaaatggcct gcgcgatgcc
      901 cgtcgcgttc accggcagga agtcgccgga agccatgtcg agcaggcgct gctcctgcac
      961 ggtggtggtg gtgggcccca tgccgtacat tccgtagtag ccgccccaac tgctgtaggt
     1021 ctggtcgtag acgatgtggg cgatggaccc catgaggccg atgcgattga agccgttgcc
     1081 gatgcgcacg tacaccgtgt tgcgctcgca gaagccccag atacggtcca tgtcgatggg
     1141 gcgggcggta ccgctgctgt cggtgtggta caatttaccg tcgctctgcc gcaggtcgtc
     1201 caccggtctt ttctcctcgt ccaagaggtc cttcagtgca acgccgggcc ggttgtcgcg
     1261 gaagtcctcg aacccgaagt acacgccttc gcggaaggtg aagccgctat catataccac
     1321 gagcgaggta tcgcgtatga tcggtgcgta gtcgatgctg tcccactggg cgccacatat
     1381 cgtcgggatc gtcgtggata tcacgagcag gatgatgcgg ttcatgccac ggaacggatc
     1441 aggtccatca
//
LOCUS       DAOQOC010000925         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74078_length_1448_cov_2.649677, whole genome shotgun sequence.
ACCESSION   DAOQOC010000925 DAOQOC010000000
VERSION     DAOQOC010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74078_length_1448_cov_2.649677"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PL106_13145"
     CDS             complement(<1..442)
                     /locus_tag="PL106_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34065.1"
                     /translation="MEKKHLKAGALLLAAAAVVSGCNGLGKMAKYASTITYTVDPSPL
                     IVKGDTVHVNINGNFPAKYFSKKALAELTPTIEGIGDQMLLATPENKVPEAYTPASAT
                     ALKMSGFQGEKAVGNYTVIPYETGKSFTYSDKVAYKPEMEHSALM"
     gene            complement(534..1226)
                     /locus_tag="PL106_13150"
     CDS             complement(534..1226)
                     /locus_tag="PL106_13150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNA34066.1"
                     /translation="MSVNVPEATHKLGGNSGDDAPVSYGYIAVEGLIGAGKTTLAKRL
                     ATRWSGRTVLEEFEDNPFLPRFYEDQHRYAFSVELSFLAQRYHQLKRVGERSLFEPIT
                     VADYSIGKSLVFASVTLPPDERALFTDLYTIMYAGLPRPDLLVYLHLPIERVRQRIRL
                     RGRSYEQAIPEVYLERLQETYLDHLQKSTGQRVLALDIGEYDLLHDAEAYERFLNLVD
                     KPRAPGFSVERL"
     gene            complement(1207..>1448)
                     /locus_tag="PL106_13155"
     CDS             complement(1207..>1448)
                     /locus_tag="PL106_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNA34067.1"
                     /translation="TQLGRTRTPGPRYTARTIDIDILLIGDLVFTGPALTVPHPRMHE
                     REFALAPAADVAPTWEHPVLRRTVLNLLNDVRQRA"
BASE COUNT          255 a          438 c          460 g          295 t
ORIGIN      
        1 gcatcagggc gctatgctcc atttcgggct tgtacgccac cttgtcgctg tacgtgaagc
       61 tcttgcccgt ctcgtagggg atcacggtgt agttgcccac ggccttctca ccttggaagc
      121 ccgacatttt caacgcggtg gcgctggccg gggtgtacgc ctcaggtacc ttgttctcag
      181 gcgtggccag cagcatctgg tcgccgatac cctcgatggt cggggtcaat tccgccaagg
      241 ccttcttgga gaaatacttg gccgggaaat tcccgttgat gttcacgtga accgtgtcac
      301 ccttcacgat cagcgggctg gggtccactg tgtaggtgat ggtcgacgcg tatttggcca
      361 ttttaccaag gccgttgcaa ccgctgacca cggccgccgc cgcgagcaac aatgcaccgg
      421 ccttgagatg cttcttttcc atgcgttttg ggggttctgg ccgcgaaagt aaccgtcagg
      481 tttccaaggg gcggcgcatc ccgacgaaca cttgtccaca gccgacgtgg ggatcataac
      541 cgctcgacgg aaaagcccgg agcacgtggt ttgtccacca aattcaggaa gcgttcatac
      601 gcctcggcat cgtgcagcag gtcgtactcg ccgatatcca gcgccagtac ccgctggccg
      661 gtcgatttct ggaggtggtc caggtaggtc tcctgcaagc gctccaggta cacctcgggg
      721 atggcttgtt cataactccg gccacgtaac cggatccgtt ggcgcacccg ctcgatcggc
      781 aagtgcaggt acaccagcaa gtcgggcctg ggcagccctg cgtacatgat cgtgtacagg
      841 tcggtgaaca acgcccgctc gtccggcggc agggtgacac tcgcgaatac cagggacttc
      901 ccgatggaat agtcggctac cgtgatcggc tcgaacaggc ttcgttcacc cacgcgtttg
      961 agctggtggt agcgctgtgc cagaaaggac agctccacgg agaaggcgta gcgatgctga
     1021 tcctcataaa agcgcggcag gaacgggttg tcctcgaatt cctccagcac ggtccgtccg
     1081 ctccagcggg tggccaaacg cttggccagg gtggtcttgc ccgcgccgat caagccttcc
     1141 acggcgatgt acccgtagct gaccggggca tcatccccgc tgttgccgcc gagcttgtgg
     1201 gtcgcttcag gcacgttgac ggacatcgtt caacaggttg agcacggtgc gacggagtac
     1261 ggggtgctcc caggtggggg ccacgtcggc tgctggggcc agggcgaact cccgctcgtg
     1321 catgcgcggg tgcggtaccg tgagcgccgg tccggtgaac accaggtccc cgatcagcag
     1381 gatgtcgatg tcgatcgtgc gggccgtgta gcgaggaccc ggcgtacgcg tacgaccgag
     1441 ctgcgttt
//
LOCUS       DAOQOC010000926         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74136_length_1447_cov_3.469109, whole genome shotgun sequence.
ACCESSION   DAOQOC010000926 DAOQOC010000000
VERSION     DAOQOC010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74136_length_1447_cov_3.469109"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PL106_13160"
     CDS             complement(<1..507)
                     /locus_tag="PL106_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34068.1"
                     /translation="MRLLNAFLAFGIVSANNAQLINGSFEDNGTFSLQGWEWVCVDPQ
                     GTQDVPIGGGIWSAWKQPGHAKGCFPSLLYQRITDVQYGIPYQLSGWVKCPQTDLQFC
                     LGASIAFGVINNGNIIDAGFTTSNDTGWTFLTTSHVFDPGIGDTAIVILSAGFTGGPM
                     NPLPAGFVT"
     gene            complement(512..1297)
                     /locus_tag="PL106_13165"
     CDS             complement(512..1297)
                     /locus_tag="PL106_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34069.1"
                     /translation="MRTSLVLLFALVYTLTRAQLVNGSFENEDGWDLTGWVSTCNNAF
                     FGIGHPDHGNWSTSVPHGETNGPCYASDLVQLVPMIQDGSTWTLDGWLHNMLTGFSDP
                     YIGMGMGIKHEDGWWEYFTSAQMNTGNWTHLTVTNTFTIAPGDTAFVVLSGGIVSGNG
                     SPAWAEFDGMVLTDLSTSIASHGANTIACFPNPATDRLWVDRSERPQRVSVIDATGRE
                     HQIERLQHNGRTLEVDVSSIPSGPYVLLMRSGTGVRSVRFIKA"
     gene            1358..>1447
                     /locus_tag="PL106_13170"
     CDS             1358..>1447
                     /locus_tag="PL106_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNA34070.1"
                     /translation="MEHLLTLDALISLVTLATLEIVLGIDNVIF"
BASE COUNT          280 a          432 c          435 g          300 t
ORIGIN      
        1 ggtcacgaac ccggcgggca aggggttcat gggaccgccc gtgaagcctg cgctgaggat
       61 cacgatggcc gtgtcgccga tgcccggatc gaagacgtgg ctcgtggtga ggaaggtcca
      121 gcccgtgtca ttgctggtgg tgaagcctgc atcgatgatg ttcccgttgt tgatgacgcc
      181 aaaggcgata ctggcaccca ggcagaactg gaggtcggtc tgcgggcatt tcacccagcc
      241 gctcaactgg taagggatgc cgtactgcac atcagttatg cgctggtaca ggagggacgg
      301 gaagcagccc ttcgcgtgcc cgggttgttt ccacgcggac cagatcccac caccgatcgg
      361 gacatcctgc gtgccttgtg gatcaacgca tacccattcc cagccttgca agctgaaggt
      421 gccgttgtcc tcgaagctgc cgttgatcag ttgggcgttg ttcgccgaaa cgataccgaa
      481 cgccaggaat gcgttgagga gcctcatgtg gtcatgcttt gatgaaacgg acggaccgaa
      541 caccggtgcc cgatctcatg agcagaacgt acggacccga cggtatgctg ctcacgtcca
      601 cttccaaggt ccggccattg tgttgcaacc tttcaatctg atgttctcga ccggtcgcat
      661 cgatcaccga cacccgctgc ggacgctctg agcgatccac ccacaaacga tcggtcgccg
      721 ggttcgggaa gcaggctatc gtgttggccc cgtgggaggc gatgctggtg gacagatcgg
      781 tgagtaccat gccgtcgaac tcggcccagg ccggtgatcc attcccgctc acgataccgc
      841 cgctcaggac gacgaatgcc gtgtcacccg gggcgatggt gaaggtgttc gtgacggtca
      901 agtgcgtcca gttgccagtg ttcatctgcg cactggtgaa atactcccac cagccgtcct
      961 cgtgtttgat gcccataccc atcccgatat acgggtctga gaacccggtg agcatgttgt
     1021 gcagccaccc gtccagcgtc caggtgctgc cgtcctggat cattggtacg agttgcacga
     1081 ggtcgctggc gtagcaaggg ccgttcgttt ccccgtgcgg caccgaagtg gaccagttgc
     1141 cgtggtccgg atgcccgatc ccgaaaaagg cgttgttgca agtggagacc cagccggtga
     1201 ggtcccagcc gtcctcgttc tcgaagctgc cgttcaccag ttgggcacgg gtcagcgtgt
     1261 aaacaagggc aaacaaaaga acgagcgaag ttctcatcgg catcagtttc gtcgaaggta
     1321 gtcgcccaaa tcgtttcttc gctgccgctt tttccacatg gaacacctgc tcacgctcga
     1381 cgcgctcatc agcctcgtta cactggccac cctggagatc gtcctgggca tcgacaacgt
     1441 catcttt
//
LOCUS       DAOQOC010000927         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74342_length_1445_cov_3.848201, whole genome shotgun sequence.
ACCESSION   DAOQOC010000927 DAOQOC010000000
VERSION     DAOQOC010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74342_length_1445_cov_3.848201"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..393
                     /locus_tag="PL106_13175"
     CDS             226..393
                     /locus_tag="PL106_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34071.1"
                     /translation="MAEEIINQGIDEGDKISIGLNKEKSDVAIKVTKGKKKIGKADDT
                     KELPPASEPGV"
     gene            complement(513..>1445)
                     /locus_tag="PL106_13180"
     CDS             complement(513..>1445)
                     /locus_tag="PL106_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNA34072.1"
                     /translation="NHSMEISPKLIISEGLRFTRVGLTSTFADNDDFGFLNGSYEQRN
                     GALTWRAGAVYMPGKGWRFSLLASSGFRAPNVDDIGKVFDSAQGDVIVPNPDLRPEYT
                     TNFEGSISRTINDRSSIEVNGFYTLYTNAITVGPYTVHGADSIDYDGVLSRVAALQNT
                     TEAYLYGASAQFIGHFNDHFTLRSGFTYTYARIRTDSTDQPLDHIPPVFGQAGLEYQA
                     KRLRLSGYVVFNGWKRLKDYNTTAGSEDNLKASTPQGSPAWYTLNVRGSFAFNAHLGL
                     QLALENITDMNYRTFGSGVSAPGRNFMASVQVRF"
BASE COUNT          273 a          436 c          459 g          277 t
ORIGIN      
        1 caatgctgcg aagggatctt tctcgagttt ggcctcacgt tccaccaatt ccaccaccag
       61 cgctgaaatg ctcttcttcc ggcggcggct caaggcggcg gcagtgcgct tgaagcggag
      121 cggaacggac aacgtgagtt tggtagtcat gtgatcacgt atagggctgt gaagttacgt
      181 gatgaggccc ctggcccgca aagacccaca tctacgcgca gggcgatggc cgaggagatc
      241 atcaaccagg gcatcgatga gggcgacaag atctccatcg ggctcaacaa ggagaagagc
      301 gacgtggcga tcaaggtgac caagggcaag aagaagatcg gcaaggcgga cgataccaag
      361 gaactgccac cggcgagcga gccgggggtg taggcgtttt ccagcccacc tgcgaaggcc
      421 gcctcagcaa gaggcggccc tattccttgc gtgatgcgcc cccgcgaaac accgtcaggc
      481 caccgtggag caccgcttcc tcattccccg cttcagaacc gcacctgcac gctcgccatg
      541 aagttccgac ctggcgcgct caccccgctc ccgaacgtcc ggtagttcat gtcggtgatg
      601 ttctccaacg ccagctgcag gccgaggtgc gcgttgaacg cgaagctgcc ccgcacgttc
      661 agcgtgtacc aggccggcga tccctgcggg gtgctcgctt tcaggttgtc ctcgctgccg
      721 gcggtggtgt tgtagtcctt cagccgcttc catccgttga acaccacgta gcccgaaaga
      781 cgcaggcgct tggcctggta ttccagaccg gcctgtccga aaaccggcgg aatgtggtcc
      841 aacggctgat ccgtgctgtc ggtgcggatc ctcgcgtagg tgtatgtgaa tccgctgcgc
      901 agggtgaaat ggtcgttgaa atggccgatg aattgcgcgc tggccccata gaggtacgcc
      961 tccgtggtgt tctgcagggc cgccacccgg ctcagcaccc catcgtaatc gatactgtcg
     1021 gcgccgtgga ccgtgtaggg ccccacagtg atggcattgg tgtacagcgt gtagaaaccg
     1081 ttgacctcaa tggaggagcg gtcgttgatg gtcctgctga tcgatccctc gaaattggtg
     1141 gtgtattcgg gtcgcaagtc ggggttgggc acgatcacat cgccctgggc gctgtcgaat
     1201 accttgccga tgtcgtccac gttgggtgcg cggaagcctg aactcgccag caggctgaag
     1261 cgccagccct tccccggcat gtacaccgcg cccgcccgcc aggtcagcgc gccgttccgc
     1321 tgttcgtagg atccgttgag gaagccaaag tcatcgttgt ccgcgaaggt ggaggtgagc
     1381 ccgacccggg tgaagcgcag gccctcggag atgatcagct tcggcgagat ctccatgctg
     1441 tggtt
//
LOCUS       DAOQOC010000928         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74354_length_1445_cov_3.176259, whole genome shotgun sequence.
ACCESSION   DAOQOC010000928 DAOQOC010000000
VERSION     DAOQOC010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74354_length_1445_cov_3.176259"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..873)
                     /locus_tag="PL106_13185"
     CDS             complement(31..873)
                     /locus_tag="PL106_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNA34073.1"
                     /translation="MEGGALVVNGELVPAGRPVIAVDNRAYRYGDGLFESIRVVNGQP
                     CFIGAHWARLTEALKVLRINCPDGLNERTLKAHIERLVVECGHASARIRLTVYRDAPG
                     HYRPEGNNSGYTIALTPLSSANYILNHEGLVTDIWPEMRKQVNRLSSLKTLNGQLYVM
                     AGLWCMDRGLDDCLLQNDKGNIIESGSGNVFIVSNGVLYTPPLSDGCIGGVMRMQVIN
                     LAVTNGIKVYETSLNPQNLLAADELIMTNAVRGPQWAAAYRTKRYTHRMALHLVDLLV
                     KSIA"
     gene            complement(873..1370)
                     /locus_tag="PL106_13190"
     CDS             complement(873..1370)
                     /locus_tag="PL106_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNA34074.1"
                     /translation="MRTRRAVFLDRDGVVVRERGEHTWRVDDLEMLPTVAEAVKAVRA
                     QGNLAVIITNQSGIALGLYTHADVERAHAWIGACLAEEGTQLDAVYYCPHHPSQGRCL
                     CRKPGSLMLERAMARYGIDPGASVMIGDRDRDVEAATAVGVRGVLVEANSPLLDVLRT
                     RTSLI"
BASE COUNT          285 a          495 c          384 g          281 t
ORIGIN      
        1 ggccaatttt ccggacgacc cttcgttgaa ttaggcgatg ctcttcacaa gcagatccac
       61 cagatgcagg gccatgcggt gcgtataacg cttggtgcgg tatgctgctg cccattgcgg
      121 cccgcgcact gcattggtca tgatcaattc atcggccgcc agcaggttct gtggattcaa
      181 tgaggtctcg tacaccttga tcccgttggt caccgccaga ttgatcacct gcatgcgcat
      241 caccccaccg atgcaaccat cgctgagcgg gggcgtgtag agcacaccat tgctcacgat
      301 gaacacgttg cccgagccgc tctcgatgat gttccccttg tcgttctgca gcaggcagtc
      361 gtccagaccg cgatccatgc accacaggcc ggccatcacg tacaactggc cattcagcgt
      421 cttcaacgaa ctgagacgat tcacctgctt gcgcatctcg ggccagatat ccgtgaccag
      481 tccctcgtga ttcaggatat aattggccga agacaacggc gtgagcgcga tcgtataacc
      541 actgttattg ccttcggggc ggtagtgtcc cggcgcatcc ctgtacaccg tgagccgtat
      601 gcgcgcactg gcatgcccgc attccaccac gaggcgttcg atgtgcgcct tcagcgttcg
      661 ctcgttcagc ccgtccggac agttgatgcg caacaccttc aacgcttcgg tgaggcgcgc
      721 ccagtgcgct ccgatgaaac acggttggcc attcaccacg cggatgctct cgaaaagccc
      781 atcaccgtac cgataggcgc ggttgtccac cgcgatcacc ggtcggcccg cagggaccag
      841 ttcaccgttc accaccaacg cccctccttc catcagatca gggatgtccg ggtccgcagt
      901 acatcgagaa gtggcgaatt ggcttccacc agcacaccac gaacgcccac cgccgtggcg
      961 gcctccacgt cgcgatcacg atcgcctatc atcaccgaag caccgggatc gatcccgtac
     1021 cgcgccatgg cccgctccaa catcagcgag cccggcttac ggcagaggca acgaccttgc
     1081 gagggatgat gcgggcagta gtacacggca tccagttgtg tcccctcttc cgccaggcat
     1141 gcaccgatcc atgcgtgcgc ccgctccaca tcggcgtgcg tgtacaaacc gagtgcgata
     1201 ccgctctggt tcgtgatgat cacggccagg ttcccttggg cccgtacagc tttcaccgcc
     1261 tcagccacgg tgggcagcat ctccaggtca tccacgcgcc aggtgtgctc gccacgctcg
     1321 cgcaccacca cgccatcgcg atccagaaaa accgcacgcc tcgtcctcat gcgaaaggtc
     1381 ctgttacggc ctgcaggaat cctccaagca caggcgggtg catcatcacc gtgaacacga
     1441 tgcct
//
LOCUS       DAOQOC010000929         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74364_length_1445_cov_2.880576, whole genome shotgun sequence.
ACCESSION   DAOQOC010000929 DAOQOC010000000
VERSION     DAOQOC010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74364_length_1445_cov_2.880576"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1445)
                     /locus_tag="PL106_13195"
     CDS             complement(<1..>1445)
                     /locus_tag="PL106_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34075.1"
                     /translation="NVNDYYGRFSVSAPDLTTWLGSCGNTRNGYDPNASNFALDAQLY
                     GLDEFTGSSCSGTRSPAVTVRNAGTTTLTSFQLSWTVNGGAGGGQSWNGSLVSGATVT
                     INLPQITLGDGPSLLSVSVGTPNGGTDQNPANDTSTSSTMFGTSMVTLQLNLDRYGAE
                     TTWRIMQGQEIVASGGPYTTQGSNGVYPQSPINVCLPDGCFELQVLDSYGDGICCNYG
                     NGNFSLTGPDGTLVTGDGQFTTQVTYPFCITSTINVSVKAMLEGPYDNVTGQMRDDLR
                     QAGIIPATEPYTALGYTHVNGGGGETVAPAVLAVTGNNAIVDWVVVELRTPGAPYTVL
                     ATRSALLQRDGDVTGTDGVSPVAFVLGNGDYQVALRHRNHLGVMTATAVALSSTAATV
                     DFRSVATGTWGTNARKTIGSTRALWAGNARLDGQVKYTGSNNDRDPILIKVGNTAPNG
                     SVNGYFPEDVNLNGSVKYTGNANDRDPILVN"
BASE COUNT          249 a          449 c          441 g          306 t
ORIGIN      
        1 gttcacaagg atggggtccc tgtcgttggc gttgcccgtg tacttcacac taccgttcag
       61 gttcacgtct tcagggaagt aaccgttcac cgaaccgttc ggcgcggtgt ttcccacctt
      121 gatcaggatg ggatcgcgat cattgttgct cccggtgtac ttcacctggc cgtcgagccg
      181 ggcattgccc gcccagagtg cccttgtgga gccgatcgtc ttccgggcgt tcgtgcccca
      241 ggtgcctgtg gccacgctgc ggaagtccac cgtggccgcg gtcgatgata gggccactgc
      301 cgtcgccgtc atcacaccga ggtggttgcg gtgtcgcaaa gccacctggt agtcgccgtt
      361 gcccaggacg aatgccacgg gcgaaacgcc atcggtcccg gtcacatccc catcacgctg
      421 gagcagggcg ctccgtgtgg ccagcaccgt gtacggggcc cctggcgtgc gaagttccac
      481 cacaacccaa tcaacaatgg cattgttccc ggtcacggcc aggacggccg gcgctaccgt
      541 ttcacccccg ccaccattca catgcgtata cccgagcgcc gtgtacggct ccgtggcggg
      601 aatgataccc gcttggcgca ggtcgtcgcg catctggccg gtaacgttat cgtacggccc
      661 ttcgagcatc gccttcacgg acacgttgat cgtgctggtg atgcagaaag gatacgtgac
      721 ttgtgtggtg aactgaccgt ccccggtgac cagcgtgcca tccggaccgg tcagggaaaa
      781 attcccgttg ccgtagttgc agcatatccc gtcgccatag ctgtcgagca cctgcaattc
      841 aaagcaacca tcgggcaggc acacgttgat gggcgattgc ggatacaccc cattgctgcc
      901 ctgggtggtg taaggaccgc cgctggccac gatctcctgg ccctgcatga tgcgccatgt
      961 ggtctccgcg ccgtaccggt ccaaattgag ttgaagggtc accatcgaag tgccgaacat
     1021 ggtgcttgag gtggacgtat cgttggccgg gttctggtcg gttccgccat tcggtgtgcc
     1081 cacggaaaca ctcaggaggc tggggccatc gcccaatgtg atctgtggca ggttgatggt
     1141 cacggtggca ccactgacga gacttccgtt ccagctctgg ccacctcctg ccccaccgtt
     1201 caccgtccac gaaagctgga atgaagtgag tgtggtggtg cccgcattgc gtaccgtgac
     1261 ggcgggtgat cgggtccctg aacaactgct gcccgtgaac tcgtcgagcc cgtacaattg
     1321 ggcgtccagc gcgaagttgg acgcattggg atcatatccg ttccgcgtgt tgccgcagga
     1381 tcccagccat gtggtcaggt ccggtgcgct gacactgaat ctcccgtagt agtcgttcac
     1441 gttgt
//
LOCUS       DAOQOC010000930         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74554_length_1443_cov_3.345821, whole genome shotgun sequence.
ACCESSION   DAOQOC010000930 DAOQOC010000000
VERSION     DAOQOC010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74554_length_1443_cov_3.345821"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            456..>1443
                     /locus_tag="PL106_13200"
     CDS             456..>1443
                     /locus_tag="PL106_13200"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNA34076.1"
                     /translation="MVTGTETAVRGTVTSTGATGYKTGVEGSASGGSGTVRGVAGIAT
                     SGGINIGTVGLASGGSIAQGTIGAATDADSNIGTDGYAVGGNLAIGARGVADGDNTAT
                     WTVGVWGESSQALSGFPSWAGYFNGPVFGTGGLLTPSDENLKSNIEDITDGLEKTLQI
                     EAKTFQYDTAGHPSMHLPGGEQSGFMAQQLESVLPHAVMNVSHPPVLDSLGNVVIEEV
                     QFKAIRTETVVPYLIGAIQQLNQKYEDLLAQVEGCCGNAGMQPQGQDRSLDIASENLV
                     RQERLLITPNPFTDHATIRYFVAAPAKVSLQVNDNAGQSIGTIREEQAMAGAF"
BASE COUNT          309 a          390 c          466 g          278 t
ORIGIN      
        1 cccaagcgct ccggttcatc tataccaacc cgtcccccgg taccaacgtg gcggccgggc
       61 tgaacggctt ggagatcgcc cgtgtgctcc ctgaccaatc cgggaatgag ggctatttcg
      121 gcatcgggga tttcgcgagt gtcacagagc aacctctgga gcgtttggat gtgctgaacg
      181 gtcgcctgcg gctgcgcgtg ttgcctgatc ccagtggcga agctgacggt acgttcaagt
      241 tgatcgtagt ggacgccagt tcaacaccga gccccgagcg gggtgtggta aaatgggtgg
      301 atccggccac atttggagaa tgtgcatccg gatggcactt ggtaggcaac aacacctgca
      361 cggcgtggga tggaaatttg tgcgttccgc agtccgagga tcttgttggt gtgggcacga
      421 acacgccatt ggcgaagttg gacgtgttga aggatgtggt cacaggaacg gagacagctg
      481 ttcgtggcac ggtgacttcc accggagcga ccggatacaa aacaggggtg gaaggcagcg
      541 cgtccggagg atccggtact gtgcggggcg ttgcaggaat tgctaccagc ggcgggatca
      601 acatagggac tgtaggcctc gcgagcggag gctcgatcgc ccagggcacg ataggggctg
      661 cgactgatgc cgacagcaat atcgggaccg atggctacgc ggtcggggga aacctggcta
      721 tcggtgcgcg cggggttgcc gatggcgata acaccgccac atggacggtg ggcgtgtggg
      781 gcgagtcgtc acaagcgctt tcgggcttcc cctcatgggc cgggtatttc aacgggcctg
      841 tcttcgggac cggcggcttg ctgactccga gcgacgagaa tttgaaatcc aatatcgagg
      901 acatcaccga cggcctcgag aaaaccctgc agatagaagc gaagactttc cagtacgata
      961 cggcgggtca tccatccatg catcttccag gtggtgaaca atccggcttc atggcccagc
     1021 aactggaatc tgtcctgcca catgccgtca tgaatgtctc tcacccgccc gtgttggaca
     1081 gcttaggcaa tgtggtgatc gaagaggtac agttcaaggc gatccggacc gaaaccgttg
     1141 tgccgtacct gataggtgcg atccagcaat tgaaccagaa atacgaggat ctgctggccc
     1201 aggtagaggg atgctgcggc aacgcgggca tgcagcccca aggtcaggat cgatcattgg
     1261 acatcgcatc ggagaacctt gtccgacagg aacgattgct gatcaccccc aatccgttca
     1321 ccgaccatgc cacgattcgg tacttcgtgg ctgcgccggc caaagtgagc ctacaagtga
     1381 acgacaacgc tggccagtct atcggcacta tacgcgagga gcaggccatg gctggtgcgt
     1441 tca
//
LOCUS       DAOQOC010000931         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74697_length_1442_cov_3.077145, whole genome shotgun sequence.
ACCESSION   DAOQOC010000931 DAOQOC010000000
VERSION     DAOQOC010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74697_length_1442_cov_3.077145"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PL106_13205"
     CDS             complement(<1..467)
                     /locus_tag="PL106_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34077.1"
                     /translation="MRLVWLGGMALLFAGPVLAQRTDLRLRVSVRCWEKDDDSTQFAV
                     SAVDTVPGSTPISTDCSVKGKCKLTLPLDHVYRVEMNAPGHVPQHVIMDLREPGIRQR
                     KWGYDTRFRVDLMPRIDSVDYSICERPLAKAYFNKRENQFVWDERYTHELEPY"
     gene            526..750
                     /locus_tag="PL106_13210"
     CDS             526..750
                     /locus_tag="PL106_13210"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNA34078.1"
                     /translation="MDHTLQVIIEHDKEGYYAFVPELPGCHTQGDSLDEVMKNIKEAV
                     DLYMGTLRPAELKRLTKKRVYTTSMPVSLG"
     gene            743..940
                     /locus_tag="PL106_13215"
     CDS             743..940
                     /locus_tag="PL106_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNA34079.1"
                     /translation="MAKQPVLTAKDAERLLKKAGFAWVRTAGGHRIYMRGNDRMVLPW
                     HVGRALHPKIARQVLDAIEEP"
     gene            937..>1442
                     /locus_tag="PL106_13220"
     CDS             937..>1442
                     /locus_tag="PL106_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNA34080.1"
                     /translation="MKISDITTLLESWAPPALQEDYDNSGLQVGTPDQEVRAALIALD
                     CTEEVVAEAVRRGCDLVIAHHPVIFRGIKSLTGRTGVERTLLAAIRHNVAIYAIHTNL
                     DNVISGVNAEIAARLGLQDLRVLDPKPHQLRKLVVFVPHAHAEAVRSALFGAGAGRIG
                     AYDECSYN"
BASE COUNT          279 a          440 c          457 g          266 t
ORIGIN      
        1 tagtaaggct ccagttcgtg ggtgtaccgc tcgtcccaca cgaactggtt ctcccgtttg
       61 ttgaaatacg ctttggcgag cggccgctcg cagatgctgt aatccacgga gtcgatgcgc
      121 ggcatcagat ctacgcgaaa tcgggtatcg tagccccact tgcgctggcg gatccccggc
      181 tctctcaggt ccatgatcac gtgctggggc acatggccgg gggcgttcat ttccacacgg
      241 tacacgtgat ccagcggcag ggtgagcttg cacttgccct tcaccgagca gtccgtggaa
      301 atgggcgtac tgccgggcac ggtgtccacg gcgctaacgg cgaattgcgt ggagtcgtcg
      361 tccttctccc aacaacgcac gctcacacgg aggcggagat ccgtgcgttg agccagcacc
      421 ggacctgcga acaagagcgc cattccccca agccacacaa gccgcatgcg ccgaagatcg
      481 ggagaagcgg ggtataggca tcctgaagta cctttgacca tcgccatgga tcataccctg
      541 caggtcatca tcgagcacga caaggagggc tattatgcct tcgttccgga attgccgggc
      601 tgccatacac agggagactc gctggatgag gtgatgaaga acatcaagga ggccgtggac
      661 ctgtacatgg gcacgttgcg acccgctgaa ttgaagcggt tgaccaagaa gcgcgtgtat
      721 accacctcga tgcctgtgag ccttggctaa gcagccggta cttaccgcca aggatgccga
      781 acgcctgttg aagaaggcgg ggttcgcttg ggtgcgcacc gccggtgggc accggattta
      841 catgcggggg aatgatcgca tggtgctgcc gtggcatgtt ggccgcgccc ttcacccgaa
      901 gatcgcccgg caggtattgg acgcgatcga ggagccatga agatcagcga catcaccacc
      961 ctcctcgaat cctgggcccc acctgccctg caggaggact atgacaacag cggcctgcag
     1021 gtcggcaccc cggatcagga ggtgagggcc gccctcatcg cgctggattg caccgaggag
     1081 gtggtggctg aagctgtgcg ccggggctgc gacctggtga tcgcccacca tcccgtcatc
     1141 ttccggggta tcaaaagcct gacggggcgc acgggcgtgg agcgcaccct gctcgcggcg
     1201 atccggcaca atgtggccat ctacgccatc cacaccaacc tggacaatgt gatctccggg
     1261 gtgaacgcgg agatcgccgc gcgcctcggc ctacaggacc tgcgggtgct cgatcccaag
     1321 ccgcaccagt tgcgcaagct ggtggtcttt gttccacatg cccatgccga ggctgtgcgc
     1381 agcgcccttt tcggtgcggg tgcgggccgc atcggggcgt acgacgagtg cagctataac
     1441 tt
//
LOCUS       DAOQOC010000932         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_74727_length_1442_cov_2.100937, whole genome shotgun sequence.
ACCESSION   DAOQOC010000932 DAOQOC010000000
VERSION     DAOQOC010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74727_length_1442_cov_2.100937"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..480
                     /locus_tag="PL106_13225"
     CDS             103..480
                     /locus_tag="PL106_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34081.1"
                     /translation="MRNTLTVRAGENFHIVLWLAKDVAWLQDWKVLGISMFLPTFLMA
                     IWIAWRLRSDRGELLHALAVVCWIAANGVWMIGEFWYADRIRHLAVPFFVAGLLCVGW
                     YYLVLLPLRIRRAGSGPHQGGLR"
     gene            580..>1442
                     /locus_tag="PL106_13230"
     CDS             580..>1442
                     /locus_tag="PL106_13230"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34082.1"
                     /translation="MPATFSFVERRVWRVLRITLWWLAAGLIFAVWESNTLEDHGHDP
                     ERLATMDHQLQRFLLIGFIGANVYIFLLRRHLRRMAYLTASGFMIALVAMILVLATVL
                     DAGNAGGTFADAIRARLISWRFVGEFLLWGILAIATMSLVRLNDQFGTGARSFLLGRY
                     FKPRQELRIFMFLDMRSSTTIAEEIGDTRYFQLLNEVYADITDPVTNHAGDIYQYVGD
                     EISVSWPLRQGVRGQRCLKCFFAIREKLRKRAAHYHQRYGFSPVFKAGLHYGQVTTGE
                     IGLVKRQTIYSG"
BASE COUNT          245 a          424 c          460 g          313 t
ORIGIN      
        1 ttggaagggg ggcgcggcga cgatacgtat cgattccaag acaccgccac cggtccacct
       61 ttggcacctt cgcgacccgt gaacaaatgc gatcatcatg ccatgagaaa taccttgacc
      121 gttcgtgctg gcgagaattt ccacatcgtg ctctggttgg cgaaggacgt ggcctggctg
      181 caggactgga aagtgctggg catctcgatg ttcctgccca cctttctgat ggcgatctgg
      241 atcgcttggc gactgcgcag tgatcgcggt gagttgctgc atgccctcgc cgtagtgtgt
      301 tggatcgcgg ctaacggggt gtggatgatc ggcgagttct ggtacgcgga caggatccgc
      361 cacctggccg taccgttctt cgttgccggg ctgctgtgcg tgggctggta ctatctggtg
      421 ctcctgcccc tgcggatccg gcgggcgggg tccggtcctc accagggtgg cctgcggtga
      481 atgggcttgg gtcgcacttt atgcgcacat ggctgatgtg gaggaaggag agtcggatga
      541 cctgttctga tcacaggccg gatagcttcg cggctcgcca tgcccgctac cttctccttc
      601 gtggaacgcc gcgtctggcg cgtattgcgc atcaccctgt ggtggctggc cgcagggctc
      661 attttcgcgg tgtgggaaag caacacgctg gaggatcacg gccacgatcc cgaacggctg
      721 gccacgatgg accaccagct ccaacgtttc ctcttgatcg gtttcatcgg tgcgaacgtg
      781 tacatattcc tgctccgtcg ccacttgcgc cgcatggcct acctgaccgc ctcggggttc
      841 atgatcgcct tggtggccat gatcctcgtg ctcgcgaccg tgctcgatgc cggaaacgcg
      901 ggcggcacct tcgcggatgc gatccgggcc cggttgatca gctggcggtt cgttggggag
      961 ttcctgcttt ggggcattct ggccatcgct accatgtccc tggtaagact gaacgaccaa
     1021 ttcggcaccg gagcacgcag cttccttctg ggccgctatt tcaagccacg ccaggagttg
     1081 cggatcttca tgttcctgga catgcgcagc agcacgacca tcgctgagga gatcggcgac
     1141 acgcgctatt tccaattgct caacgaggtg tacgccgaca tcacggatcc cgtgaccaac
     1201 catgcggggg atatttacca gtacgtcggg gatgagatca gcgtgagctg gccgttgcgc
     1261 cagggggtgc gcgggcagcg gtgcctgaag tgcttcttcg cgatccgcga gaaactgcgc
     1321 aaacgtgccg cgcattacca ccaacgctac ggattctccc ccgtgttcaa ggccggcctg
     1381 cactatgggc aggtgaccac gggagagatc ggtctggtga aacgccagac catatacagt
     1441 gg
//
LOCUS       DAOQOC010000933         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_75115_length_1438_cov_2.832972, whole genome shotgun sequence.
ACCESSION   DAOQOC010000933 DAOQOC010000000
VERSION     DAOQOC010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75115_length_1438_cov_2.832972"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PL106_13235"
     CDS             complement(<1..601)
                     /locus_tag="PL106_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA34083.1"
                     /translation="MPSLILITGATSGFGEAMARRFAADNWRVIITGRRTERLEALRT
                     ELDGLHGTGTVHALRFDVRDRAAVEHAIASLPEEWRPIDVLVNNAGLAAGFAPIQHGH
                     PEDQDIMIDTNVKGLLHVSRAVIPGMIERKRGHIINIGSLAGKEVYPNGNVYCATKHA
                     VDALTKGMRQDLLPYGIKVTQIAPGLAETEFSIVRFHGDE"
     gene            complement(823..>1438)
                     /locus_tag="PL106_13240"
     CDS             complement(823..>1438)
                     /locus_tag="PL106_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNA34084.1"
                     /translation="FIVARRLMYWQVYLHKTVVACEMMLVETLRRAKTLGLADVPLFG
                     SPALLRFLRTRHDRASFNDPAVLTDFLKLDDHDIMGAVKVWCDHPDKVLARLAADLTQ
                     RHNLHIRLRDARWTDEEVQALRTHVSNTLGITPEESAHFVLTGRIVNNAYDPAKDRIE
                     LLFKDGTVRDIAQASDNLGIAALARPVEKWYLAWPRGVNGVVPG"
BASE COUNT          250 a          447 c          465 g          276 t
ORIGIN      
        1 cctcatcgcc gtggaagcgt acgatgctga actccgtttc ggccaggccc ggggcgatct
       61 gcgtcacctt gatcccgtac ggcagcaggt cctgtcgcat gcccttggtg agcgcgtcca
      121 ccgcatgttt ggtggcgcaa tacacgttgc cgttgggata cacctccttg cccgcgaggc
      181 tgccgatgtt gatgatgtgg ccccgcttcc tttcgatcat accggggatc accgcccggc
      241 tcacgtggag cagtcccttc acgttggtat cgatcataat gtcttgatct tcgggatggc
      301 cgtgctggat cggcgcgaag cccgcggcga gccccgcgtt gttcacgagg acatcgatcg
      361 ggcgccactc ctccggcaac gaggcgatcg cgtgttccac ggcggccctg tcgcgcacat
      421 cgaaccgcag ggcgtgtacg gtgcccgtac cgtgcaagcc gtccagttcc gttcgcaacg
      481 cttccaatcg ttcggtacgg cgaccggtga tgatgacacg ccagttatca gcggcgaagc
      541 gacgtgccat ggcttcgccg aagccgcttg tggcacccgt gatcaggatg agggaaggca
      601 tggggcaaag gaaggagcct tcctgcccat cccgtcacat ttcagaatgg acgttcacag
      661 cctgtccgca caccgttccg gcgatggaag accgcgcatg tgggctggag gatcaagcgg
      721 ttcaggtgtg ttccggctgg tggatgccaa ctggatgatc gacatcgcgc ttcgacgtcg
      781 gttgtctaag tgcgcacgat cccgtgcatg cgcacgcagc gatcaacctg gaacaacgcc
      841 attgaccccc ctgggccagg ccaggtacca tttctcaacc ggccgggcga gcgcagcgat
      901 accgaggttg tcgctggcct gggcgatgtc gcgcacggta ccatccttga acagcagttc
      961 gatgcggtcc ttggcggggt cgtatgcgtt gttcacgatg cgaccggtaa gcacgaagtg
     1021 ggccgactcc tccggggtga tccccaacgt gttcgacacg tgcgtccgca gggcctgcac
     1081 ctcctcatcg gtccaacggg catcgcgcaa gcggatgtgc aggttgtggc gctgggtgag
     1141 atcggcggcc aggcgggcca ataccttgtc cgggtgatcg caccagacct tcacggcacc
     1201 catgatgtcg tgatcgtcca gcttgaggaa gtccgtcagc acggccgggt cgttgaagct
     1261 cgcgcgatcg tgccgtgtgc gcaggaagcg aagcagggcc ggggagccga agagcggcac
     1321 atcggccagg ccgagggttt tggcgcgtcg gagcgtttcc accagcatca tctcgcaggc
     1381 caccacggtc ttgtgcaggt acacctgcca gtacatgagc cggcgcgcca cgatgaac
//
LOCUS       DAOQOC010000934         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_75313_length_1436_cov_4.184649, whole genome shotgun sequence.
ACCESSION   DAOQOC010000934 DAOQOC010000000
VERSION     DAOQOC010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75313_length_1436_cov_4.184649"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PL106_13245"
     CDS             complement(<1..646)
                     /locus_tag="PL106_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA34085.1"
                     /translation="MFKNFIQRPVLAIVISILTVFMGMLAIEQLPTSQFPEIAPTTVN
                     VFIAYPGASADVLTKSCVVQLETAINGVQGMRYIASDATSAGEATIRIIFEPGTDPNM
                     AVVRVKTRVDQVMPNLPPLVQREGVIITPVQPSMLMYVNLYGDAKDADERFLYNYAYT
                     QLIPEIQRIPGVAQAQILGTRVYAMRIWLKPDRMRAYNISAEEAMEAMKEQSLIA"
     gene            complement(767..>1436)
                     /locus_tag="PL106_13250"
     CDS             complement(767..>1436)
                     /locus_tag="PL106_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA34086.1"
                     /translation="KAELAAAQAHLSFTEIHASFNGIVGRFHVRKGSLLDEGELLTEL
                     SDNSSMWVYFNVPEAEYLAYRRNEQAGNGTKVRLMLADTEIFDQVGEITAIESDFNNR
                     TGNIAFRATFANPKGVLRHGQTGNILMESRLDHVLLIPQKATFEVLDHRYVFLIDKND
                     TLRQTRIETSGELQHIFVVNKGLTKDDHFLLEGLRKVKDRQKVEPVLVPPDSVIKHLE
                     LYAE"
BASE COUNT          261 a          425 c          431 g          319 t
ORIGIN      
        1 gtgcgatgag gctctgctcc ttcatcgctt ccatggcctc ctcggccgag atattgtagg
       61 cccgcatgcg gtccggtttc aaccagatgc gcatggcgta cacccgggta ccgaggatct
      121 gcgcctgtgc caccccgggg atccgctgga tctcggggat caactgcgtg tacgcgtagt
      181 tgtacaggaa gcgctcgtcc gcatccttgg catcgccgta caggttcacg tacatgagca
      241 tgctgggctg cacgggagtg atgatcaccc cttcgcgctg caccaggggc ggcaggttcg
      301 gcatcacctg gtccacgcgc gtcttcacgc ggaccaccgc catgttcggg tcggtgcccg
      361 gttcgaagat gatgcggatg gtggcttcac cggcactggt ggcgtccgag gcgatgtacc
      421 gcatgccctg cacaccgttg atcgccgttt ccagttgcac cacgcatgat tttgtgagca
      481 catcggcgct cgctccggga taggcgatga acacgttcac cgtggtgggc gcgatctccg
      541 ggaattgtga ggtgggcaac tgttcgatcg ccagcatccc catgaacacc gtgaggatcg
      601 agatgacgat ggccaggacc ggccgttgga tgaagttctt gaacatggat cgaaaagttt
      661 gggatcactt caccacagag gcacagaggg cacggaggaa gagtttgaaa ccggctatgt
      721 atctctccgt gtcctctgtg cctctgtggt gaagtactca gaaggttcac tcggcgtaca
      781 actccaggtg cttgatcacc gaatcgggcg ggacgaggac cggctccact ttctgcctgt
      841 ccttcacctt ccgcaaaccc tccaacagga agtggtcgtc cttggtgagg cccttgttca
      901 ccacgaaaat gtgctggagt tcgccactgg tctcgatgcg tgtctgccga agggtgtcgt
      961 tcttgtctat caggaagaca tacctgtgat ccagtacctc gaaagtggcc ttctgcggga
     1021 tcaacaggac atgatccaga cgggactcca tgaggatgtt gcccgtttgc ccatgccgga
     1081 gcacgccttt cggattggcg aaggtggccc ggaaggcgat gttgcccgtt ctgttgttga
     1141 agtcgctctc gatcgcggtg atctccccga cctggtcgaa gatctccgtg tcggccagca
     1201 tcagccgcac cttcgtgccg ttcccggcct gctcgttccg ccgataggcc agatactccg
     1261 cttcaggtac attgaagtac acccacatgc tgctgttgtc cgagagttca gtgagcagct
     1321 cgccctcgtc caacaaactg cctttccgga cgtggaagcg gcccacgatc ccgttgaagg
     1381 acgcatggat ctccgtgaaa ctcaggtgcg cttgggccgc ggccagttcg gccttg
//
LOCUS       DAOQOC010000935         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_75400_length_1436_cov_2.020999, whole genome shotgun sequence.
ACCESSION   DAOQOC010000935 DAOQOC010000000
VERSION     DAOQOC010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75400_length_1436_cov_2.020999"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PL106_13255"
     CDS             <1..507
                     /locus_tag="PL106_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34087.1"
                     /translation="TDRIGASSREMIDQMNDIVWSVDPKNDDAGALSERLRAFASDLL
                     AARGIALDFRADEGVNERKLSAEQRRNVFLISKELLHNTVKYADARQVNIMLATAGRS
                     LELTIGDDGKGFDPDNTDSYDGNGLPNMRARAAAVGAEFTIDSAPGKGTRARIVLAHQ
                     VFTPRSGD"
     gene            565..1209
                     /locus_tag="PL106_13260"
     CDS             565..1209
                     /locus_tag="PL106_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA34088.1"
                     /translation="MRTIHVALFDDSKQIRDSLGTLLSTTPGIGLCGCYPDAVNVTAK
                     VAACKPDVVLMDIDMPGVNGIDAVRTLHAERPELPIMMLTTFADEERIYDSLRAGAVG
                     YLLKNNDPGAVLDAIQEVHAGGAPMTPSIARKVMLHFQRPEATTELPDVGLSPREKEV
                     LTALVDGLSYKMIAERLGISFETVRSHIKKIYEKLHVHNNAEAVAKTLKGRLLG"
     gene            complement(1214..>1436)
                     /locus_tag="PL106_13265"
     CDS             complement(1214..>1436)
                     /locus_tag="PL106_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34089.1"
                     /translation="LSDGMPKHMALRRAKLDHLDGAGDELARPSYWAGLVVGDTAPIE
                     GLSGTGHVLWWVLGGLGAAGLILWWWRKR"
BASE COUNT          289 a          440 c          470 g          237 t
ORIGIN      
        1 accgatcgga tcggggcgtc ctcccgtgag atgatcgatc agatgaacga catcgtctgg
       61 agcgtggatc cgaagaacga tgatgccggg gcactgagcg aacgcctgcg ggcgttcgcc
      121 agtgacctgc tggccgcgcg cggcatcgcg ctcgatttcc gtgcggacga gggtgtcaat
      181 gagcgcaagc tttccgctga acagcgcagg aacgtttttc tgatcagcaa ggagctgttg
      241 cacaacacgg tgaagtatgc tgacgcacgc caggtgaaca tcatgcttgc gaccgcagga
      301 cgttcattgg agttgacgat cggtgatgat ggaaagggct tcgatccgga caacaccgac
      361 agctacgacg gcaatggcct gccgaacatg cgcgcgcggg cggccgcggt cggagcggag
      421 ttcaccatcg acagtgcgcc cggcaaggga acgcgcgcgc gcatcgtgct cgcgcatcag
      481 gtgttcaccc cccgttctgg ggattgatgc ggccaggacc gcgggtagtt ttgccggacc
      541 tcccgacccc gcactcgcca ggccatgcgc accatccacg tcgcgctgtt cgatgacagc
      601 aagcagatcc gcgattcgct gggcacgctg ctgagcacca ctccgggtat cggtctttgc
      661 ggctgctacc cggatgcggt gaacgtaacg gcgaaggtcg cggcgtgcaa accggacgtg
      721 gtgctcatgg acatcgacat gcctggcgtg aacgggatcg atgcggtgcg caccctgcac
      781 gcggagcggc cggagctgcc gatcatgatg ctcaccacct tcgccgatga ggaacgcata
      841 tacgactcat tacgggcggg agccgtgggc tacctgctga agaacaacga tccgggtgcg
      901 gtgctcgacg ccatccagga ggtgcacgct ggtggcgcac ccatgacgcc gagcatcgca
      961 cgcaaagtga tgctgcattt ccaaaggcct gaagcgacca cggaactgcc cgacgtcggc
     1021 ctgagcccgc gcgagaagga agtactcacc gcgctggtcg acgggttgag ctacaagatg
     1081 atcgccgagc ggttggggat cagtttcgag acggtgcgca gccacatcaa gaagatctac
     1141 gagaagttgc acgtgcacaa caatgctgag gccgttgcga agaccttgaa aggacggttg
     1201 ctgggttgag cgatcatcgc ttccgccacc accagaggat cagcccggcg gcccccagcc
     1261 caccgagcac ccaccacagg acatggcccg ttccactcag gccttcgatg ggtgccgtat
     1321 cgcccaccac caggccggcc caatacgagg ggcgcgcgag ttcatcaccg gcgccatcga
     1381 ggtggtcgag cttggcgcgg cgcagggcca tgtgtttcgg catcccatcg gacagg
//
LOCUS       DAOQOC010000936         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_75538_length_1434_cov_3.012328, whole genome shotgun sequence.
ACCESSION   DAOQOC010000936 DAOQOC010000000
VERSION     DAOQOC010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75538_length_1434_cov_3.012328"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..904
                     /locus_tag="PL106_13270"
     CDS             278..904
                     /locus_tag="PL106_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34090.1"
                     /translation="MPSTLQRKIAILKNAAVAGKVHAFEEFESNVPYMYLDVAGQVTV
                     GIGHAILSEEAACQLPFKSADGTPASREVIVAAFKKIHSGRQAQREKAKPASSFAGLT
                     DLILAETDIYRLAQQDIASHEHLLRGKFPDYDRYPAAVQEALLDMIFSMGLNKLMTGY
                     PRFVAAVRAQDWATAAAHSGRRQVQGSRNLAIAGKLRSAAGNGATAAR"
     gene            1182..>1434
                     /gene="tssD"
                     /locus_tag="PL106_13275"
     CDS             1182..>1434
                     /gene="tssD"
                     /locus_tag="PL106_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HNA34091.1"
                     /translation="MAFRADLSMNGKTYRLLHCSYALSRDVDPTGRPASEVKGGTISF
                     EIESTEDTSIWDLMIAQFKSVDGTITFKKRDEDAKMKELK"
BASE COUNT          328 a          437 c          404 g          265 t
ORIGIN      
        1 caatgactct tgcgaccatg accttccaaa tataaacgcc tgaacggagc atggaaccgt
       61 gcttcgcgat cgtcgttcct tgaaccacca ctgctgttcc cttgtgcgat cctgctgatg
      121 cttgacccgg aacgtccttc atgccgcatc cgatcacctt tggttccaga tccttcgacc
      181 cggaccttcc gatcatcggt aaggagtagg ctccggccct ttgaacgatg cagtgtatcc
      241 gtgcatttgc ggatcccgcc caacaaacac cagcatcatg ccttccacgc tccaacgcaa
      301 gatcgcgatc ctcaagaacg ccgcagttgc ggggaaagtc catgccttcg aggaattcga
      361 atcgaatgtg ccctacatgt acctggatgt cgcaggccag gtgaccgtgg gcatcggcca
      421 tgcgatattg agcgaggagg cggcctgtca gttgccgttc aaaagcgcgg acgggacacc
      481 ggcatcgcga gaggtgatcg tggcagcatt caaaaagata cactccgggc gtcaagccca
      541 acgcgagaag gccaagcccg cctcttcctt cgccggactg accgatctga tcctcgccga
      601 gactgacatc taccgcttgg cgcagcagga catcgcgtcg cacgaacacc tgctacgcgg
      661 aaaattcccg gactacgaca ggtatcccgc agcggttcaa gaagccttgc tggacatgat
      721 cttcagcatg ggcttgaaca agctgatgac gggctacccc aggttcgtgg cggccgtgcg
      781 tgcgcaggat tgggccactg cggccgccca ttccggtcga aggcaggtac aaggatcacg
      841 caacctggcg atcgcgggca agttgcgaag tgctgcgggc aacggtgcga cggccgcacg
      901 atgaatggcc atggtccact tcttgggcaa ggctccggac gtgcttactg ttttcaccgg
      961 tcgggtgcat catgcaagcc acacggtgcg gggtcgcaag tcgcatgcga accgtgcccg
     1021 ccggaagatc gccgttcgtt gaacacgctg aacggttgaa cggtcatccc ggtacgttca
     1081 tttgatcccg accccggcca ccggatgaac ccgcatcttt gatccatacg gtacgaacag
     1141 tacacaaaga acggacctga aaaccaataa acccaacgaa catggcattc cgagcagacc
     1201 tgagcatgaa tggcaaaacc taccgcctgc tgcactgcag ctacgcgctg agccgcgatg
     1261 tggaccctac cggccgcccc gcgagcgagg tgaaaggcgg cacgatctcc tttgagatcg
     1321 agagcaccga ggacaccagt atctgggacc tgatgatcgc ccagttcaag agcgtggatg
     1381 gcaccatcac cttcaagaag cgcgacgagg acgccaagat gaaggagttg aagt
//
LOCUS       DAOQOC010000937         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_75808_length_1431_cov_5.774709, whole genome shotgun sequence.
ACCESSION   DAOQOC010000937 DAOQOC010000000
VERSION     DAOQOC010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75808_length_1431_cov_5.774709"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PL106_13280"
     CDS             <1..932
                     /locus_tag="PL106_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNA34092.1"
                     /translation="KNTSDPIELMMKAQVVNSTGEAWEKVDLSLSSGNPTLGGNMPTM
                     SAWVLQQPYTLRTTSTRRKGRSMFEAQAAPPAMADGAELKETESMRYEDIDAKLELEN
                     TVVYNTTTVEFAIDAPFTVPADGLAHTVAVRTHKVPATYKHYGTPKLDKDAFLYARTT
                     GWEDLNLIPGQANVFFEGTYVGQSYLNVQSTSDTLNVSLGRDKGVVVERVRRKSTNDK
                     AIIGGKRTINVGFDISVRNTKSSAIDIEVRDQHPLSPRSEIEVHLEEKGDAVVNEQTG
                     QLTWNIRVEPKSTKKLGFAYSVKHPKDMPVVVE"
     gene            959..>1431
                     /locus_tag="PL106_13285"
     CDS             959..>1431
                     /locus_tag="PL106_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34093.1"
                     /translation="MKSGSIAMGIGPCLVPVLLSAQTVDSVLLEEQSFTHLPFVYSVY
                     SSVIDRNGDHYLYTTGLEYGLRGYDIEDPTVPGEVFAFQPSSFNGLKPTNLFQQDQFL
                     FVSLGAFHGATQNAGMATVDISDPANPAILDQWDSAVFTTGTAIVRVQDGYAYLG"
BASE COUNT          336 a          429 c          423 g          243 t
ORIGIN      
        1 cgaagaatac gagcgacccc atcgagctga tgatgaaagc gcaagtggtg aacagcacgg
       61 gcgaggcctg ggagaaagtg gacctgtccc tcagcagtgg caaccccacg ctcggtggca
      121 acatgcccac gatgagcgcc tgggtgctgc aacaacccta caccttgcgc accacatcca
      181 cccgcaggaa aggcaggtcc atgttcgaag cccaggccgc tcccccggcc atggccgatg
      241 gtgccgaact gaaggaaacg gaatcgatgc gctatgagga cattgacgcc aaactagaat
      301 tggagaacac cgtggtgtac aacactacca ccgtggagtt cgccattgat gcgcccttca
      361 ccgtgcccgc cgatggcctg gcccacaccg tggccgtgag gacgcacaag gtgcccgcga
      421 cgtacaagca ctacgggacg ccaaaactcg ataaggacgc cttcctctat gcgcgcacca
      481 ccggatggga ggacctgaac ctgatccccg gacaggcgaa tgttttcttc gaagggacct
      541 atgtcgggca aagttacttg aacgtgcaaa gcacgtccga cacgctcaat gtgtccctgg
      601 gccgcgacaa gggcgtggtg gtggagcgtg tccgccggaa gagcaccaac gacaaggcca
      661 ttatcggcgg caaacgcacg atcaacgtgg gcttcgacat cagcgtgcgc aacaccaaga
      721 gcagcgcgat cgacatcgag gtgcgcgacc agcacccgct gagcccgcgc agcgagatcg
      781 aggtccatct ggaggaaaag ggtgacgccg tggtgaacga gcagaccggc cagctcacgt
      841 ggaacatccg cgtggagccg aagagcacca agaagctggg attcgcttac agtgtgaagc
      901 acccgaagga catgccggtg gtggtggagt aaccggaaca ggatactacc tttcggtcat
      961 gaagtccgga agtatcgcga tggggatcgg gccttgcctg gtccccgtcc tgctttccgc
     1021 acaaaccgtt gacagtgttc ttctggagga acagagcttc acccacctgc ccttcgtgta
     1081 ttcggtctac tcgtcggtga tcgatcggaa cggggaccac tacctgtaca ccacgggact
     1141 ggagtacggc ctgcgcggtt acgacatcga agacccgaca gttcccggtg aggtgttcgc
     1201 attccaacca tcatcgttca acgggctcaa accaacaaac ctgttccaac aggatcaatt
     1261 cctgttcgtg agcctcggtg cgttccatgg tgccacccag aatgccggta tggccaccgt
     1321 ggatatcagc gatccggcga accccgcaat actcgatcaa tgggacagtg ccgtattcac
     1381 aacgggcacg gcgatcgtgc gtgtgcagga tggatacgcc tatctcggca t
//
LOCUS       DAOQOC010000938         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76063_length_1429_cov_3.147016, whole genome shotgun sequence.
ACCESSION   DAOQOC010000938 DAOQOC010000000
VERSION     DAOQOC010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76063_length_1429_cov_3.147016"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..>1429)
                     /gene="yidC"
                     /locus_tag="PL106_13290"
     CDS             complement(33..>1429)
                     /gene="yidC"
                     /locus_tag="PL106_13290"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNA34094.1"
                     /translation="YTGDLHFTAEKLGTTGLRLKASTDDPSRYLAITYQLDSIGWLMR
                     MNTEVVGLQNEVDPRNMVFQWDVLGRSHEKHLPTEQRKCTVFYKYMTEDRSYLSETGD
                     EHLKLEGRTNWVAFKQDFFTLALIRPDGFRGNGSEIGVAMRNDGLHTKAYSAKLFFDE
                     APAAHASLPMTLYLGPNHFNTLRRTEIPQFDRIIDLGWGIFGWVNRFIVIPIFNFLGK
                     FNLSYGIIILVLTVAIKLLLLPIAYRNQKSSARMRALKPELDALGKKFPATEDMMKKQ
                     QAQMELYRKAGVSPMAGCVPMLLQLPILYAMFQFFPASIELRQQPFLWADDLSSYDSI
                     ATLPFTIPLGYGDHVSLFTILMCASTVVYTMITSKQMPQQQGMPNMKLMMYLFPVMML
                     FFMNSLPAGLSLYYLLANIISILQMTVLGRFFINEDKLRAELVTNMSKPRKKSKWQQR
                     IEEMQKQQQAARKR"
BASE COUNT          247 a          419 c          452 g          311 t
ORIGIN      
        1 cagggcgttg cctcctgctc ctgctgtccg acctacctct tccgcgccgc ctgctgctgc
       61 ttctgcatct cctcgatgcg ctgctgccac ttgctcttct tgcgcggctt gctcatgttg
      121 gtcaccagtt cggcgcgcag cttgtcctcg ttgatgaaga agcggcccag cacggtcatc
      181 tgcaggatgc tgatgatgtt ggccagcagg tagtacaggc tgaggcccgc cggcaggctg
      241 ttcatgaaga agagcatcat caccgggaac aggtacatca tcagcttcat gttgggcatg
      301 ccctgttgtt gcggcatctg cttgctggtg atcatggtgt acaccacggt gctggcgcac
      361 atcaggatgg tgaacaggct cacgtggtcg ccgtacccca gcgggatggt gaagggcagg
      421 gtggcgatgc tgtcgtagct gctcaggtcg tccgcccaca ggaagggctg ctggcgcaat
      481 tcgatgctgg ccgggaagaa ctggaacatg gcatagagga tgggcagctg caacagcatg
      541 ggaacgcagc cggccatggg gctcacaccg gccttgcggt acaattccat ctgcgcctgc
      601 tgcttcttca tcatgtcctc cgtggccggg aacttcttgc ccagcgcgtc cagctccggt
      661 ttcagggccc gcatgcgcgc gctgctcttc tggttgcggt aggcgatggg caacaacaag
      721 agcttgatcg ccacggtgag caccaggatg atgatgccgt aactcaggtt gaatttcccc
      781 aggaagttga agatcgggat gacgatgaaa cggttcaccc agccgaagat gccccacccc
      841 aggtcgatga tccggtcgaa ttgcgggatc tccgtgcgcc gcagcgtgtt gaaatggttg
      901 gggcccaggt acagggtcat gggcaggctg gcgtgggcgg cgggcgcctc gtcgaagaag
      961 agcttggcac tgtacgcctt ggtgtgcagg ccgtcgttgc gcatggccac gccgatctca
     1021 cttccattgc ctcggaagcc gtccgggcgg atcagggcca gggtgaagaa gtcctgcttg
     1081 aaggccaccc agttggtacg tccctccagc ttcaaatgct cgtcgcccgt ctcgctcaga
     1141 tagctgcggt cctcggtcat gtatttgtag aagaccgtgc atttgcgctg ttccgtaggc
     1201 aggtgtttct cgtggctgcg ccccagcacg tcccattgga agaccatgtt ccgcgggtcg
     1261 acctcgttct gcaaccccac gacctcggtg ttcatccgca tcaaccagcc gatactgtcc
     1321 aattggtagg tgatcgcgag gtagcgggag gggtcgtccg tggatgcctt cagccgcaga
     1381 ccggtggtgc ccagcttctc ggccgtgaaa tgcaggtcac cggtgtaca
//
LOCUS       DAOQOC010000939         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76148_length_1428_cov_3.809905, whole genome shotgun sequence.
ACCESSION   DAOQOC010000939 DAOQOC010000000
VERSION     DAOQOC010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76148_length_1428_cov_3.809905"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PL106_13295"
     CDS             <1..1329
                     /locus_tag="PL106_13295"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA34095.1"
                     /translation="AHCLVAATCIFGMAGAQHITGSLPPGSATDGLIHLRISMGSSTR
                     ALDSTTIRPDGHFAFQPRTFQTGFYDLVINDSDRVDIILVPGEKNVELAFNGTPLQRH
                     LSVVRSDENKRLWEYKLVSKESQAIQAAATADRHGLQVNDFARMRELDSISARAVQLK
                     QAHLQGIINGFPNSYLAKVIRADQGLDDARDKNPLAVMKVFDFSDPSLVHSSIYDKAV
                     MTFLRNIHASSEEQFISASDSLIGYASRDPDCRAYMIEHLIDLFSTYGPELPLDHIID
                     HYVVSTEAIAMMDPALRAKVTEIRKVSVGAEAPDLDLPVVDGPPIPLYAVVKEHRYTV
                     LFFYGSTCDHCHKEIPVLKEVWNAFRPKGVQVLGIALDPDSTEFRKTIDEMELPWPAY
                     SEFNGWGSKLAKALRVRATPWFYVLDRNMRIVAKPVDATALGLWLNEHVK"
BASE COUNT          288 a          463 c          403 g          274 t
ORIGIN      
        1 gcccactgcc tcgtcgccgc gacctgcatc ttcggcatgg ccggggcgca gcacatcacc
       61 ggctccctgc ctccggggtc cgccaccgat ggccttattc acctccgcat ctccatgggc
      121 tcttccacgc gggccttgga ttccaccacc atccggcctg atgggcattt cgcattccaa
      181 ccacgcacgt tccaaacggg tttctacgac ctggtcatca acgattcgga tcgtgtggac
      241 atcatccttg tccccgggga gaagaacgtg gaactggcct tcaacggaac gccgctgcaa
      301 cggcaccttt ccgtcgtacg atccgacgag aacaagcggc tgtgggagta caagctcgta
      361 tccaaggagt cccaggcgat acaggccgcg gctaccgccg atcggcacgg tctccaggtg
      421 aatgacttcg cgcgcatgcg cgaactcgac agcatttcgg cccgtgccgt ccaactgaag
      481 caggcccatc tgcagggcat catcaacggg ttcccgaaca gctacctcgc caaggtgatc
      541 cgtgccgatc aagggctcga tgatgcccgc gacaagaacc ccctcgccgt gatgaaggtc
      601 ttcgacttca gcgacccttc gctggtacac agctcgatct atgacaaggc tgtgatgacc
      661 ttcctgcgga acatccatgc cagcagcgag gaacagttca tttccgccag cgattcactc
      721 atcgggtacg cgagccgtga tccggattgc cgggcgtaca tgatcgaaca tctcatcgat
      781 ctgttctcca cctatggacc ggagttgccc ttggaccata tcatcgatca ctacgtggtg
      841 tccaccgaag cgatcgcgat gatggatccc gcactccgcg cgaaggtgac cgagatccgc
      901 aaggtgtcgg tcggggccga agcgccggac cttgatcttc ccgtagtgga cgggccgccg
      961 atcccgcttt acgccgtggt gaaggagcat cgctacaccg tgcttttctt ctacgggagc
     1021 acctgcgatc attgccacaa ggaaatacct gtactcaagg aggtatggaa cgcgttccgc
     1081 ccgaagggcg tccaggtcct cggtatcgcc cttgacccgg acagcacgga gttccggaag
     1141 acgatcgacg agatggaact gccctggccg gcctatagcg agttcaatgg ctggggctcc
     1201 aagctggcca aggcattgcg cgtgcgggcc acgccctggt tctatgtgtt ggaccggaac
     1261 atgcgcatcg tggccaaacc cgtggacgcc acagcgctcg ggctatggtt gaacgaacat
     1321 gtgaagtagt tgaggagcgc agaaaccctg tgaggcctct ggtccggcgg ccgatccgcg
     1381 ggataagctc accctaccga tcacaacccc aatacccacc ccttgtac
//
LOCUS       DAOQOC010000940         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76377_length_1426_cov_2.881838, whole genome shotgun sequence.
ACCESSION   DAOQOC010000940 DAOQOC010000000
VERSION     DAOQOC010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76377_length_1426_cov_2.881838"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PL106_13300"
     CDS             complement(<1..278)
                     /locus_tag="PL106_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34096.1"
                     /translation="MLRSMTGFGRSEGTVAGKKVTVEIRSLNSKQLDLFIKLPPVYRE
                     KDAELRQWAADGLVRGKVELVITSEPVPGAERRRFNEALILEYYQELHR"
     gene            364..>1426
                     /locus_tag="PL106_13305"
     CDS             364..>1426
                     /locus_tag="PL106_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA34097.1"
                     /translation="MRFGTKAIHAGVEPDPSTGAIMTPIFQTSTYVQAAPGDHKGYEY
                     SRTHNPTRTALQKALAALENGKHGLCFASGMASIDAVIKLLAPGDEVVSTNDLYGGTY
                     RLFTRIFEGFGVKFRFVDMTDPAHVAKAITARTKLVWVETPTNPMLNIIDIAAIGALA
                     KKAGAWLGVDNTFASPYLQTPLDLGADIVMHSVTKYLGGHSDVVMGALVVKDDVLHER
                     LAFIQNSCGATPGPQDCFLVLRGLKTLHLRVQRHCENGAVIAEFLAAHPKVDKLYWPG
                     LPTHRNHDVAKKQMRGFGGMISFTLKGDRMDDATRVLASTKLFSLAESLGGVESLLGH
                     PASMTHASIPREERLKSGVA"
BASE COUNT          275 a          463 c          440 g          248 t
ORIGIN      
        1 cgatgcagtt cctggtagta ttcaaggatg agcgcctcgt tgaagcgacg ccgctccgcc
       61 cccggtacgg gctcgctggt gatcaccagc tccaccttgc cccgcaccag tccgtcggcg
      121 gcccactggc gcagttcggc gtccttctcg cggtacaccg gcggcagttt gatgaacagg
      181 tccagttgtt tgctgttgag cgagcggatc tccaccgtca ccttcttccc ggcaacggtc
      241 ccctccgagc ggccgaagcc ggtcatcgaa cgaagcatcg cgggcgaaag tagtcggcag
      301 gcgttgcccg caaacgtgat gcggcttccc gctaccttcg cccgccttca acgccagaat
      361 accatgcgct tcggtacaaa agcgatccac gccggtgtgg aacccgatcc gtccacgggg
      421 gccatcatga cgcccatctt ccaaacgagc acgtacgtgc aggccgcacc gggcgaccat
      481 aaaggctacg agtacagccg cacacacaac cccacgcgca cggcgctgca gaaagcgctg
      541 gccgccttgg agaacgggaa gcacggcctg tgcttcgcca gcggtatggc ctcgatcgat
      601 gcggtgatca aattgcttgc acccggtgac gaggtggtga gcaccaacga cctgtacggt
      661 ggcacctacc ggctcttcac aaggatcttc gaaggcttcg gtgtgaagtt ccgcttcgtg
      721 gacatgaccg acccggcaca tgtggccaag gccatcacgg ccaggaccaa gctggtctgg
      781 gtggaaacgc ccaccaaccc gatgctcaac atcatcgaca tcgcggccat cggcgccttg
      841 gccaagaagg cgggcgcatg gctcggggtg gacaacacct tcgcttcacc atatctgcaa
      901 acgcccctgg acctgggtgc ggacatcgtg atgcacagtg tgaccaagta tctcggcggt
      961 catagcgacg tggtcatggg tgcgctggtg gtgaaggacg atgtactgca tgagcgcctg
     1021 gccttcatcc agaacagctg cggcgccacg cccgggccgc aggactgctt cctggtgctg
     1081 cgcgggctga agaccttgca tctgcgtgtg cagcgccact gcgagaacgg tgcggtcatc
     1141 gcggagttcc tggccgcgca ccccaaggtg gacaagttgt actggcccgg cttgcccacg
     1201 caccgcaacc acgatgtggc caagaaacag atgcgcggct tcggcggcat gatcagcttc
     1261 accctcaagg gcgaccgcat ggacgatgcc acacgggtgc tcgccagtac caagttgttc
     1321 tcgttggccg agagcctcgg aggggtggaa tcgctgctgg gccacccggc cagcatgacg
     1381 cacgccagca ttcctcgcga agagcgtctg aaaagtggtg tggccg
//
LOCUS       DAOQOC010000941         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76393_length_1426_cov_2.582057, whole genome shotgun sequence.
ACCESSION   DAOQOC010000941 DAOQOC010000000
VERSION     DAOQOC010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76393_length_1426_cov_2.582057"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PL106_13310"
     CDS             complement(<1..925)
                     /locus_tag="PL106_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNA34098.1"
                     /translation="MKQILSALLVVASTSCAIGQVPSYVPTNGLVGWWPFNGNANDES
                     GNANHGTPYGPILTTDRNNVADNAYAFNRSAVQTIDVSQSPTLNGCPALTISLWTILN
                     SYAANGQAGYNHFVNKSPGGSDYQYALANNTTGLYFYYGSGGAYFQTSTLPSLNAWHH
                     LALTYTYDGTPTNSWCRFYVDGACTDSFPTTTVLQPTTHPLRFGSFPGGTYNTVDGKV
                     DDLGIWNRMLTANEVSGLFSETPVSPCISSTPVSFIGLNTSYTINDGPSTLVGTPSGG
                     VFIGLGVSGNSFDPAVAGIGIHSITYTYLDGT"
     gene            1030..1383
                     /locus_tag="PL106_13315"
     CDS             1030..1383
                     /locus_tag="PL106_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA34099.1"
                     /translation="MEKKLGQKIRMVRELKGFSQEYMAHALGLSQRGYSKLERDETKL
                     DWERINQISQVFEMDPMDLVSFDDNLVFHNCTQSGKANTIYNQIPEKLMAQYEERIKH
                     LEGEVAFLRSELARR"
BASE COUNT          305 a          379 c          437 g          305 t
ORIGIN      
        1 tggtgccatc gaggtaggtg tacgtgatgc tgtggatgcc gataccggcc accgcagggt
       61 cgaagctatt gccgctcact cccaggccga tgaacacacc gccggaaggc gttccgacca
      121 aggtggacgg gccgtcatta atggtgtacg aggtgttcaa cccgatgaag ctgacggggg
      181 tgctactaat gcagggcgac actggagtct ctgagaagag cccacttact tcgttcgcag
      241 tcaacatacg gttccagatc cctaggtcat ccaccttacc atccaccgtg ttgtacgtgc
      301 cccctggaaa ggacccgaag cgcaacggat gcgtcgtagg ctgaaggacg gtggtggtgg
      361 ggaaactgtc cgtgcaagca ccgtccacat agaaccggca ccaggaattg gtcggtgtac
      421 catcgtaggt ataggtgagc gccagatggt gccatgcgtt cagcgagggc agcgtgcttg
      481 tctggaagta ggcgccacca ctgccgtagt agaagtatag gccggtggta ttattggcta
      541 gggcgtattg gtagtctgat cccccggggc tcttgttcac gaagtgattg tacccggcct
      601 ggccgtttgc tgcgtagctg ttcaggattg tccataagga gatggtcaga gccggacacc
      661 cattcagcgt gggtgattgc gacacgtcga tggtttggac cgcgctgcgg ttgaacgcat
      721 aggcgttgtc cgctacgtta ttgcgatctg tagtgagtat tgggccataa ggcgtgccgt
      781 gattggcgtt cccgctttcg tcatttgcat tcccattgaa tggccaccaa ccaacaagtc
      841 cgttggtggg cacatagctc ggcacctggc cgatcgcgca ggaggtgctt gcgactacaa
      901 gaagtgcgga tagaatctgt ttcatcgtcg tggtcggttc ggtgcgaaca aagctcaaca
      961 gtgcgcttgt tcgccgctca cccgattcgt tctattcgcc ttatgtttgg ttctaaatcc
     1021 ggacgacgca tggagaagaa gcttggccag aagatccgca tggtgcgcga gttgaagggc
     1081 ttctcgcagg agtacatggc ccatgcactc ggcctgagcc agcggggcta cagcaagctc
     1141 gagcgggacg agaccaaact ggactgggaa cggatcaacc agatctcgca ggtcttcgag
     1201 atggatccca tggacctcgt ctccttcgac gacaacctgg tcttccacaa ctgcacgcag
     1261 tcgggtaaag cgaacacgat ctacaaccag atcccggaga agctgatggc gcaatacgag
     1321 gagcgcatca agcacctgga gggcgaagtg gccttcctgc ggagcgaact tgcgaggcgg
     1381 tgagaacttc tcggctccaa ctttgctcaa taacccccgc ggacat
//
LOCUS       DAOQOC010000942         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76403_length_1426_cov_2.401167, whole genome shotgun sequence.
ACCESSION   DAOQOC010000942 DAOQOC010000000
VERSION     DAOQOC010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76403_length_1426_cov_2.401167"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1015)
                     /locus_tag="PL106_13320"
     CDS             complement(98..1015)
                     /locus_tag="PL106_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA34100.1"
                     /translation="MEIVVPRIDITGPELLNHQRWVNKYLEDMNRRLPPGGTFMVQVD
                     TAAGRKERILARWPRPLNTLIYTVDYLTHRVWPKLPGLRGLYFAMTKGRDRVMSEMEV
                     IGRLYACGFRLLETGHAGDGRVSMLVEKTGEPAFDPHATYGPLIRLRRVGKGGKMITV
                     YKFRTMSPYSEYIQAYIHERNGYDGEGGYVDDTRITTAGAFMRRYWLDELPMVWNLIK
                     GDLKLFGVRPVSRPYFNMFPPAFQEYRKQFLPGLIPPIVVDRPDTFEDIWLSEKKYLD
                     AYAKAPLRTDLRYCWKAIWNIVVRRVRSS"
     gene            complement(991..>1426)
                     /locus_tag="PL106_13325"
     CDS             complement(991..>1426)
                     /locus_tag="PL106_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34101.1"
                     /translation="RNLERLRKVVPVTSICMHGSPRSKFDNKELWVRRDYRDLGILGE
                     PYLDVDFQRVAYYTDTGGRWDGGRYSVRDHAPVMPADPYSTTNDMISALRHGNFPRTA
                     MLTFHPQRWTDSPALWLRQRVAQTAKNYAKLGVIRWRSLSRA"
BASE COUNT          241 a          465 c          436 g          284 t
ORIGIN      
        1 tggccggtct ggctgtagtg gatgacgagg acccgcttca tgacacctgg gaacggccaa
       61 atatcggctg agcaccgcgt gcggccttct tcgaggatca ggaactgcgc acccgccgca
      121 ccacgatgtt ccagatcgcc ttccagcagt accgcagatc ggtgcgcagc ggtgccttcg
      181 cataggcgtc caggtatttt ttctcgctca accagatgtc ctcgaaggtg tcgggccggt
      241 ccaccacgat cggggggatg agcccgggca ggaactgttt gcggtattcc tggaacgccg
      301 gcgggaacat gttgaagtaa gggcggctca ccggtcgcac cccgaagagc ttcaggtcgc
      361 ccttgatcag gttccacacc atgggcaact cgtccagcca gtagcgccgc atgaaggccc
      421 cggccgttgt gatccgggtg tcgtccacat agcccccttc accgtcgtac ccgttgcgct
      481 cgtggatgta cgcctggatg tactcggaat acggcgacat cgtccggaac ttgtacacgg
      541 tgatcatttt gccccccttg cccacgcgcc gcagcctgat caacggtccg taggtggcat
      601 gcgggtcgaa ggcgggttca ccggtctttt ccaccagcat gctcacgcgg ccatcgccgg
      661 cgtgtccggt ctccagcagc ctgaacccgc aggcgtacaa gcggccgatg acctccattt
      721 ccgacatcac ccgatcgcgc cccttggtca tggcgaagta caggccgcgc aatcccggca
      781 gcttgggcca cacgcgatgg gtcaggtaat cgaccgtgta gatgagcgtg ttcagcggtc
      841 ggggccagcg ggccaagatg cgctccttcc gcccggcggc ggtgtccacc tgcaccatga
      901 atgtcccgcc cggcggcagc ctccggttca tgtcctccag gtacttgttc acccaccgct
      961 ggtggttcaa cagttccggc ccggtaatgt ctatgcgcgg gacaacgatc tccatcggat
     1021 cacaccgagt ttcgcgtagt ttttagcggt ctgtgccacg cgctgccgca accagagcgc
     1081 cggagagtcc gtccaccgtt gtggatggaa ggtgagcatg gccgtgcgcg ggaagttccc
     1141 gtggcgcaag gcgctgatca tgtcgttcgt ggtgctgtac gggtcggccg gcatcacggg
     1201 cgcgtggtcg cgcacgctgt atcgcccacc gtcccacctt ccgccggtgt ccgtgtaata
     1261 ggccacccgt tggaagtcca catccaggta cggttcgccc aggatcccca ggtcccgata
     1321 gtccctgcgc acccacaact ccttgttgtc gaatttggat cgcggactgc cgtgcatgca
     1381 gatgctggtg accgggacca ccttgcgcag acgttccaag ttgcgc
//
LOCUS       DAOQOC010000943         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76441_length_1425_cov_9.567153, whole genome shotgun sequence.
ACCESSION   DAOQOC010000943 DAOQOC010000000
VERSION     DAOQOC010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76441_length_1425_cov_9.567153"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1425
                     /locus_tag="PL106_13330"
     CDS             <1..>1425
                     /locus_tag="PL106_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34102.1"
                     /translation="GHTFPNIPGTYHWYPDDNPFGNCGNVLFTGDLIIPPDVTLFIHN
                     MTLRFAPEARMIVRAKGYVSAIACYLTNEDCLGPRWPGIRVEGDPNDPLQSEILQGRL
                     QLSHTTVDDAVVGVWCSSDDGPGHTGGIVRTYWSTFSNCIVGARIEQYHRIINEVEVV
                     NKCSFNSTSFDVTLSWPDLQELPQAQIHLFDVNGVAVNRCDFSNVYPVEFMEPHQRGV
                     GILSFDASYRCKGFGDYANNRFRRLAVGALNIWNDPLFANQVDGMHFDRNLVGLYDMG
                     CLYSMVTNNRFTTMANTEPVSFLSLGMYIDQSAGYLVERNIFEDVDLSLPHEVHSVGI
                     WFHGDQPVENRIYDNAFYGLTLGNVAEGVHHHTTGTVPPVMTGLQWLCNLYEGGFVDQ
                     FLLSPDGSIKQQQGVNNPLATAGNVFVGQKDCQTQYEPVVFADHVDEYVIDNVVVPYV
                     VTYHYYAHDQSPDCRPECVEDENN"
BASE COUNT          299 a          444 c          393 g          289 t
ORIGIN      
        1 cggccacacc ttccccaaca tccccggcac ctaccattgg taccccgatg acaacccctt
       61 tggcaattgc ggcaatgtgc tcttcaccgg tgatctgatc atcccaccgg atgtcactct
      121 gttcattcac aacatgaccc tgcgcttcgc acccgaggcc cgcatgatcg taagggccaa
      181 aggatacgtc tcggccatag catgttacct gaccaacgag gattgcctgg gtccgcgttg
      241 gcccgggatc cgggtagagg gggatccgaa cgacccgcta cagagcgaga tcctccaagg
      301 ccgcctacag ttgtcccata ccacggtgga tgatgccgtg gtgggcgtgt ggtgcagcag
      361 cgacgatggg ccaggccata ccgggggcat tgtgcgcacc tactggtcca ccttcagcaa
      421 ttgcatcgtt ggtgcacgga tcgaacaata ccatcggatc atcaatgagg tcgaagtggt
      481 gaacaaatgc tccttcaaca gcaccagctt cgatgtcaca ctgtcctggc ccgatctgca
      541 ggagttgccc caagcacaga tccacctctt tgatgtgaac ggcgtggccg tgaaccgttg
      601 cgacttctcc aacgtgtatc ctgtggagtt tatggagccg caccaacgcg gcgtgggcat
      661 cctctccttc gatgcctcct accgctgcaa gggtttcggc gactatgcca acaaccggtt
      721 ccgccgactg gccgtggggg ccctgaacat ctggaacgat ccgctcttcg ccaaccaggt
      781 ggacggcatg cacttcgacc gtaacttggt gggcctctac gacatgggct gcctctacag
      841 catggtcacc aacaaccggt tcaccaccat ggccaacaca gagcctgtca gcttcctgag
      901 cctgggcatg tacatcgatc agagcgcggg ctacctcgtg gagcgcaaca tcttcgagga
      961 tgtcgatctc tcactgcccc acgaggtgca cagtgtgggc atctggttcc atggtgacca
     1021 acccgtagag aaccggatct acgacaacgc cttttacgga cttacgttgg gcaacgtggc
     1081 cgagggcgtg catcaccata ccacggggac ggtaccccca gttatgaccg ggctgcaatg
     1141 gctttgcaac ctgtatgagg gcggtttcgt cgatcagttc ctgctctccc cggacggctc
     1201 catcaagcag cagcaaggcg tgaacaatcc gcttgccacc gctggcaacg tcttcgtggg
     1261 ccagaaggat tgccagaccc agtatgagcc ggtggtcttc gcggaccatg tcgatgagta
     1321 tgtgatcgac aatgtggtgg taccctacgt ggtcacctac cactactacg cccacgacca
     1381 aagccccgat tgccgaccgg agtgcgtgga ggatgagaac aacat
//
LOCUS       DAOQOC010000944         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76554_length_1424_cov_3.596056, whole genome shotgun sequence.
ACCESSION   DAOQOC010000944 DAOQOC010000000
VERSION     DAOQOC010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76554_length_1424_cov_3.596056"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PL106_13335"
     CDS             <1..259
                     /locus_tag="PL106_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA34103.1"
                     /translation="PPILILDEATSALDTESERLVQEALVKMLEGRTSLVIAHRLSTI
                     QHCDEICVMGQGRIIERGTHDQLYAAGGQYRKLCDMQAFGQ"
     gene            complement(318..>1424)
                     /locus_tag="PL106_13340"
     CDS             complement(318..>1424)
                     /locus_tag="PL106_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNA34104.1"
                     /translation="VDTLSYRDSLWHYHKMLHGAVLMMEPGTGAVRAWIGGNDHRYLP
                     YDQVRARRSVASTIKPAVYAAAIERGLQPCDYFSNEKKTYAAFDDWAPDNFGHDTTDS
                     QVAMWYALARSMNRPTVDLYFQTGTDTLRRTFAALGLPTTDVDKPAVALGASSVALET
                     LVPAYGAFANRGKRLPPRLITRITDAQGYVIHEAKAPKAVQALKESTAAAIAVMLQRA
                     VNEGTGTALRTRYGLSGPWAGKTGTAQDYSDAWFVAFTPGLVIGTHVGGFDPSVHFQS
                     AHGTGGALALPIAGQVLQRIERTPDLRGKYVRSFDWADAYAIDLDCDPRRERSPFGEW
                     VKDVFRPKGSRADSLPAAPKKDKGFFDRLFNRED"
BASE COUNT          227 a          454 c          483 g          260 t
ORIGIN      
        1 cccgccgatc ctgatcctgg acgaggccac cagcgcgctg gacaccgaga gcgaacgcct
       61 ggtgcaggag gccctggtca agatgctcga aggtcgtacg agccttgtga tcgcacaccg
      121 gctgagcacg atccagcatt gcgacgagat ctgcgtgatg ggccagggcc ggatcatcga
      181 gcgtggcacg catgatcagc tgtacgcagc gggcgggcag taccgcaagc tgtgcgacat
      241 gcaggcgttc gggcagtgat ccatacggaa cggttcgtgc ggtgcccccc agcggtcggc
      301 gatcgcgtcc gttgggatca gtcttcccgg ttgaacaaac ggtcgaagaa tcccttgtcc
      361 ttcttcggtg cggccggcaa gctgtccgct cgggagcctt tcggccggaa cacatccttc
      421 acccattcac cgaagggtga acgttcccgc cgtgggtcgc aatccaggtc gatcgcatac
      481 gcatcggccc agtcgaacga tcgcacgtac ttcccgcgca ggtcgggcgt ccgttcgatc
      541 cgttgcagca cctgcccggc gataggcagg gcaagtgcgc ctccggtacc atgggcactt
      601 tgaaaatgta ctgacgggtc gaaaccgccc acatgggtgc cgatcaccag gcccggcgtg
      661 aaggccacga accaggcatc gctgtagtcc tgtgcggtgc cggtcttgcc cgcccaggga
      721 cccgagagcc cgtatcgtgt gcgcagggcg gtgccggtgc cctcgttcac cgcccgctgg
      781 agcatgaccg cgatcgcggc cgccgtggac tccttgaggg cctgcacggc cttgggggcc
      841 ttggcctcgt ggatcacata gccctgcgca tcggtgatcc gggtgatcaa ccggggtggt
      901 agccgcttgc cgcggttggc gaaggcccca tacgccggga ccagcgtttc cagcgccacg
      961 ctggacgcac cgagggccac agcgggtttg tcgacatcgg tggtggggag accgagtgcg
     1021 gcgaaggtgc gcctcagtgt atccgtaccg gtctggaagt ataggtctac cgtgggccgg
     1081 ttcatgctgc gggccagggc gtaccacatg gccacctggc tgtccgtggt gtcgtgcccg
     1141 aagttgtccg gggcccaatc gtcgaatgcg gcgtaggtct tcttctcgtt gctgaagtag
     1201 tcgcagggct gcaggccccg ttcgatcgcg gccgcgtaca cggccggctt gatggtgctc
     1261 gccacggagc gtcgggcccg cacctgatcg tagggcaggt agcggtggtc gttcccgccg
     1321 atccacgcgc gcacggctcc ggtcccgggt tccatcatga gcacggcgcc gtgcagcatc
     1381 ttgtggtagt gccacaggct gtcccggtag ctcagtgtat ccac
//
LOCUS       DAOQOC010000945         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76669_length_1423_cov_3.618421, whole genome shotgun sequence.
ACCESSION   DAOQOC010000945 DAOQOC010000000
VERSION     DAOQOC010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76669_length_1423_cov_3.618421"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PL106_13345"
     CDS             complement(<1..376)
                     /locus_tag="PL106_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNA34105.1"
                     /translation="MTDKNAVTVFEEKQVRTLWDGEQEKWYFSIIDVIAALTGNERPR
                     KYWNDLKTKLAKEGSQLSDKIGQLKLRSSDGKWYATDVADTEQLFRLIQSIPSPKAEP
                     FKLWLAQVASERLDEMADPEITI"
     gene            complement(519..674)
                     /locus_tag="PL106_13350"
     CDS             complement(519..674)
                     /locus_tag="PL106_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34106.1"
                     /translation="MRSYTFLSVLRAVQERPRCYRVYENSDKLKITGTAVKNLGEAKR
                     ALESVVA"
     gene            complement(695..1042)
                     /locus_tag="PL106_13355"
     CDS             complement(695..1042)
                     /locus_tag="PL106_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA34107.1"
                     /translation="MDTSAMVDFVKGVDAVAAALTGKEAQISIITEIEFLSWPGLSEE
                     RIPEARAFLNEYASNGIGDFIRDYSAWLKRTYKLKLPDAVIAATAKNLNVPLLTRDKG
                     FNKIAHLIDVRLI"
     gene            complement(1053..1280)
                     /locus_tag="PL106_13360"
     CDS             complement(1053..1280)
                     /locus_tag="PL106_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34108.1"
                     /translation="MPKKNKPVNAGTARVRPAPKRKARAIKKVAAAPRAKRGKHEAPP
                     QRLFDAMKWFGAFPELAGPTLKVQRQLRDEW"
BASE COUNT          239 a          439 c          403 g          342 t
ORIGIN      
        1 cgatggtgat ctccgggtcg gccatctcgt cgagccgttc cgaagccact tgcgccagcc
       61 acagtttgaa gggttcggcc ttgggagagg ggatggactg gatcagacgg aagagttgtt
      121 cggtgtcggc cacgtctgtg gcgtaccact tgccgtcaga ggacctcaat ttcagttgtc
      181 cgattttatc ggacaactgg ctgccttcct tggccagctt ggttttcagg tcattccagt
      241 acttgcgcgg gcgctcgttg ccggtgaggg ccgcgatcac atcgatgatg gagaaatacc
      301 acttctcctg ctcaccgtcc cacagggtgc gcacctgctt ctcctcgaag accgtgacgg
      361 cgttcttgtc ggtcatgata gcggcaaggt aggctggttg gagcttgatc gatcactcat
      421 gaccgatcgt gcgttttcag tggtccggga tttccggaca actggatgga gccattccgg
      481 ggagcctaac ccccaccacc cctttcaccc gccaaagcct aagcgacaac ggactccagc
      541 gcccttttcg cctccccgag gttcttcacc gccgtcccgg tgatcttcag cttgtcgctg
      601 ttttcgtaca ccctgtagca cctgggcctt tcctgcactg cgcgcagcac ggacaggaag
      661 gtgtagctcc gcaaggttgc gttcctgctg tccgtcagat caaccgcacg tcgatcaggt
      721 gtgcgatctt gttgaagcct ttgtcgcgcg tcagcaaggg gacattcaag ttcttggcgg
      781 tggccgcgat cacggcatcg ggcagtttga gcttgtaggt gcgcttgagc caggcggagt
      841 agtcgcggat gaaatccccg ataccgttcg aggcatattc attgaggaag gcacgtgctt
      901 cggggatgcg ctcctccgaa aggccgggcc aggacaggaa ttcgatctcg gtgatgatgg
      961 agatctgtgc ctccttgcct gtcaatgcgg ctgcaacggc atccacccct ttcacgaagt
     1021 ctaccatcgc actggtatcc acgagaacgc gctcaccact catcgcgcag ttgccgttgc
     1081 accttcagcg ttggtcctgc cagttccggg aatgcaccga accacttcat cgcatcgaac
     1141 aacctttgtg gcggtgcttc gtgcttgccc cgctttgccc gtggtgccgc cgcaaccttc
     1201 ttgatcgctc gcgccttccg tttgggtgcc ggtcgcacac gcgcggtacc tgcgttcacc
     1261 ggcttgttct tcttgggcat ggtgccacga aggtagttca ggacagggcc ttcattccat
     1321 ctcgctctcc aacaactcag gccgctttgc ccgaagccaa cgggatcccg gccagttcct
     1381 tgcccttgcc cttcagctct tcttccaggt cgaagtcgtt ctg
//
LOCUS       DAOQOC010000946         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76676_length_1423_cov_3.415205, whole genome shotgun sequence.
ACCESSION   DAOQOC010000946 DAOQOC010000000
VERSION     DAOQOC010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76676_length_1423_cov_3.415205"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>1423)
                     /gene="pnp"
                     /locus_tag="PL106_13365"
     CDS             complement(152..>1423)
                     /gene="pnp"
                     /locus_tag="PL106_13365"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNA34109.1"
                     /translation="EVDVLPGTHGSAVFTRGETQAINTVTLGSKMDEQTVDTAMVKRS
                     DAFMLHYNFPSFSTGEVKPIRGPGRREIGHGNLALRALKPVVPNSPENPYTVRLSCDI
                     LESNGSSSMATVCSGTLALMDAGVRIKAPVSGIAMGMISDPDGKYAILSDILGDEDHL
                     GDMDFKVTGTEKGITATQMDMKVDGLSYEVLAQALEQARAGRLHILAEMLKTMDKPRA
                     DYKEHAPRIISIEIPKETIGPVIGPGGRVIQEIQAETNTTIVLEEKDGVGHVQIMSEN
                     KAGIDAALARVRAIAFPPQAEVGQTYHGKVKTIMPYGAFVEIFPGTDGLLHVSELEWR
                     RIDRVEDVLKEGDMIDFQVVGKDPRTGKLKLSRRVLLPKPEGWVDREPVMEGERRDRG
                     DRPRRDDRPRRDAGGYRDRGPRRDDRGPDNN"
BASE COUNT          205 a          441 c          493 g          284 t
ORIGIN      
        1 ggttcaacgc accggtgtgc ccagcggtta cggcggacgt ggagataggc ggcggcgcac
       61 acgatggatc cggatgcgcg gatgccggaa cggacagggc cgctcccctt gcggagagcg
      121 gccctgcgtt acggtcaggt ccggcgcccg atcagttgtt gtccggacca cgatcatccc
      181 gacgcgggcc acgatcgcgg tagccgccgg catcgcggcg ggggcgatca tcccgacggg
      241 ggcggtcgcc acggtcacgg cgttcgcctt ccatcacggg ttcgcggtcc acccaacctt
      301 cgggtttggg cagcagcaca cggcggctga gtttgagctt gccggtgcgg gggtccttgc
      361 ccaccacctg gaagtcgatc atgtcgccct ccttgagcac atcctccacg cggtcgatgc
      421 ggcgccactc gagttcgctc acgtgcagca ggccatcggt gccggggaag atctccacga
      481 aggcgccata gggcatgatg gtcttcacct tgccgtggta ggtctggccc acttcggcct
      541 ggggcgggaa ggcgatggcg cgtacacggg cgagggcggc gtcgataccg gccttgttct
      601 cgctcatgat ctgcacatgg cccacgccgt ccttctcctc caatacgatg gtggtgttgg
      661 tctcggcctg gatctcctgg ataacgcgtc cgccggggcc gatcaccggg ccaatggtct
      721 ccttggggat ctcgatgctg atgatacgtg gcgcatgctc cttgtagtcg gcgcgtggct
      781 tgtccatcgt tttcagcatc tcggccagga tgtgcaggcg gcctgcgcgg gcctgttcga
      841 gggcttgggc gagcacctcg tagctcaggc cgtccacctt catgtccatc tgggtggcgg
      901 tgatgccttt ctcggtgccg gtcaccttga aatccatgtc gcccaaatga tcctcgtcgc
      961 cgaggatgtc actgaggatg gcgtacttgc cgtcggggtc gctgatcatg cccatggcga
     1021 taccgctcac gggtgccttg atccggaccc ccgcatccat cagggcgagc gtaccggagc
     1081 acaccgtggc catcgaactg gagccgttgc tctcgaggat gtcgcagctg aggcgcacgg
     1141 tataggggtt ctcaggcgag ttgggtacca cgggtttgag cgcgcggagc gcgaggttgc
     1201 cgtggccgat ctcccgacgt ccggggccgc ggatgggttt cacctcgccg gtgctgaagc
     1261 tggggaagtt gtagtgcagc atgaaggcat cgctgcgctt gaccatggcg gtgtccacgg
     1321 tctgctcatc catcttggag ccgagggtca cggtgttgat cgcttgggtc tcgccgcggg
     1381 tgaagacggc gctgccgtgg gtgccgggca gcacgtccac ctc
//
LOCUS       DAOQOC010000947         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76848_length_1422_cov_1.958303, whole genome shotgun sequence.
ACCESSION   DAOQOC010000947 DAOQOC010000000
VERSION     DAOQOC010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76848_length_1422_cov_1.958303"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PL106_13370"
     CDS             complement(<1..577)
                     /locus_tag="PL106_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34110.1"
                     /translation="MKPPRHLHQALLLCAALLSANTHAQRARYWTGGSGNWSDPAHWS
                     LVPGGPGGAGAPRASENALFRGTEGTISVHVADDARCHDLLMDAGQGTVILEGDPSAS
                     LTLGGELRLTGAVDWRFTGPVVCSADQGTIALGTNGIPVSSDFILDGSGTWELRSALV
                     LTEDADLVLKHGALNLNDGVLRTGGLRTEGRA"
     gene            complement(574..>1422)
                     /locus_tag="PL106_13375"
     CDS             complement(574..>1422)
                     /locus_tag="PL106_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNA34111.1"
                     /translation="QFSVNPAVAGSKDCLDVRLGFRKQWLGFPGSPSTGWATLHGAIR
                     NKKKPFVKNRHGIGAFIESDKAGAYGYSHLYLAYAYHIQMAQDYMISMGFFAGIKQFK
                     LDLGELDPLMPGDPLLANRASVMVWPEVWPGLWLYSKNGWAGLSMNQVLSNRVKDFGE
                     NTHLTRHFLLSAGRRLRINRTTGLVGSTLLKYSPRSPIALDINVMLEYKRKLGLGISY
                     RNQDAIAFMVKVPFLKYFALGYSYDLTTSRLKRKSTGSHEVILAIYPCGSPDPVRDIV
                     RCPVFE"
BASE COUNT          274 a          454 c          432 g          262 t
ORIGIN      
        1 gggcacggcc ttcggtgcgc agtccacccg tacgcagaac accgtcgttc aggttgagcg
       61 cgccatgctt caggaccagg tccgcatcct ccgtcagtac cagcgcgctg cgcaattccc
      121 acgttccgct cccatcgaga ataaaatcgc tggatacagg tataccgttc gttcccaacg
      181 cgatggtgcc ctggtccgcg gagcacacca ccgggccggt gaaccgccag tcgaccgcac
      241 ccgtgaggcg caattcgccc ccgagcgtga gggatgccga tgggtcgccc tcgagaatga
      301 cggttccctg accggcatcc atcagcagat cgtggcaccg cgcatcatcc gccacatgca
      361 cggagatggt cccttccgtg ccgcggaaca acgcattttc cgatgcgcgg ggtgcgccgg
      421 caccacccgg accgccgggc acgagcgacc aatgggccgg atcgctccag ttgccgctgc
      481 cgcccgtcca gtacctggca cgctgcgcat gggtgttcgc ggagaggagc gcggcgcaga
      541 gcaggagcgc ctggtggagg tggcgtggtg gcttcattcg aacacggggc atcggacgat
      601 gtccctgacc ggatcgggac tgccgcacgg ataaatggcg aggataactt cgtgcgaacc
      661 agtacttttc cgtttcaggc ggctggtggt caggtcgtaa ctgtatccca gggcgaaata
      721 cttcaggaag ggcaccttca ccatgaaggc gatggcgtcc tggttgcggt acgagatgcc
      781 gaggcccagc ttccgcttgt actcgagcat cacattgatg tccaaggcga tgggcgaccg
      841 cggcgaatac ttcaacagcg tgctgcccac caggcccgtg gtgcggttga tccgcaaccg
      901 gcgaccggca ctcaacagga aatgacgggt gaggtgcgtg ttctccccga aatccttcac
      961 cctgttgctc agcacctggt tcatcgaaag acccgcccaa ccgttcttgc tgtacaacca
     1021 gagccctggc catacctccg gccataccat cacgctcgcc cggttggcca gcaagggatc
     1081 cccgggcatc agcgggtcca gctccccgag gtcgagtttg aattgcttga tcccggcgaa
     1141 aaaacccatg ctgatcatgt agtcctgtgc catctggatg tgataggcat aggccaagta
     1201 aagatgcgaa tagccgtatg cccccgcctt gtcgctctcg atgaaggcac cgatgccgtg
     1261 gcggttcttg acgaacggct tcttcttgtt ccggatagcc ccgtgcagtg tggcccaacc
     1321 cgtggaaggg gaaccgggaa atccgagcca ttgcttgcgg aagccgaggc gtacgtccag
     1381 gcaatccttg ctgccggcaa ccgccgggtt cacactgaac tg
//
LOCUS       DAOQOC010000948         1421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_76940_length_1421_cov_2.629575, whole genome shotgun sequence.
ACCESSION   DAOQOC010000948 DAOQOC010000000
VERSION     DAOQOC010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76940_length_1421_cov_2.629575"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PL106_13380"
     CDS             <1..540
                     /locus_tag="PL106_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34112.1"
                     /translation="ADQCRANFTSRADTAFIAQLDAMYRRDQITRDGSAEMLRNDSLN
                     FEELITRCDAHGFPSAVEIGPSIGVVHLLLWHHRGPEYPDSPQWQRILPFIHTAMDAG
                     RLDPAFLCMFDDLNDKDHGRPMRYGALLGYYRNMPEEVFLVDPVTLTMNRASVGWGPI
                     ADFADVVGVDPRLIRYAVK"
     gene            complement(653..>1421)
                     /locus_tag="PL106_13385"
     CDS             complement(653..>1421)
                     /locus_tag="PL106_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34113.1"
                     /translation="LYLTDPGQTGNTGTLGATVFLGGPINWGTNLMSDALRSGGLIPT
                     TEPYTAMGYALENAGATVSASVLSTTGNNAPVDWVVVELKNATGGYPTVARRACLVRR
                     NGTVITPDGNTVITFTTTTTVGKHLVISHRNHLAVMSGAPIATNGQVIDFSTVAATTL
                     YGTNAMQVNGSRRALWPGRVNSDVMVKYTGGGNDRDPLLTLIGSGTPNASVPGYRRED
                     LNMDGAVRYTGSGNDRDLVLGTVGSTAPGATRTQQVP"
BASE COUNT          266 a          454 c          432 g          269 t
ORIGIN      
        1 gcagaccaat gcagggcgaa cttcacctcc cgagcggaca ccgcattcat cgcacaactt
       61 gatgcgatgt accgccggga ccagatcaca cgcgatggaa gcgcggagat gttgcggaac
      121 gacagcctga acttcgagga actcatcacc cggtgcgacg cacatggttt tccgagcgcg
      181 gtcgagatcg gtccttccat cggcgtggta catctgctgc tctggcatca ccgtggcccc
      241 gaatacccgg acagcccgca atggcagcgg atcctgccct ttatccacac ggccatggat
      301 gccggtcgtt tggatcccgc tttcctctgc atgttcgacg acctgaacga caaggaccac
      361 ggccgcccca tgcgatacgg tgccttgctg ggatactacc gcaacatgcc cgaagaggtg
      421 ttcctggtgg atccggtcac cttaacgatg aaccgggcct cggtgggctg gggaccgatc
      481 gcggacttcg cggacgtggt gggcgtggat ccacggctga tccgttatgc cgtcaaatag
      541 taccatctgg tcattcaata ccagccaccc cacctcccgc aagcaggaag aaacacttcc
      601 tggataggac atgcaccacg cgacgaacgg gtgctggctc gttccaggcg gttcagggca
      661 cctgttgtgt gcgcgtggca ccgggcgctg tcgaacccac cgtgcccaat acaaggtcgc
      721 ggtcgttgcc gctgcccgtg tatcgcaccg caccatccat gttcagatcc tcccggcgat
      781 atccgggtac cgatgcgtta ggcgtaccgg aaccgatcaa ggtaagcaac ggatcacgat
      841 cgttgcctcc gccggtatac ttcaccatga catcggaatt gacgcggccg ggccatagcg
      901 cgcggcgcga tccgttcact tgcatggcat tggtcccgta cagcgtggtg gcggccaccg
      961 tggagaagtc gatcacctgg ccgttagtgg cgatgggcgc acccgacatc accgcgaggt
     1021 ggttgcggtg ggagatcacc aagtgcttgc ccaccgtggt agtagtagtg aacgtgatca
     1081 ccgtgttccc atccggggtg atcaccgtgc cgttgcgccg caccaggcag gcacgacggg
     1141 caaccgtggg atatccgccc gtggcattct tcagttccac caccacccaa tccaccgggg
     1201 cgttgttgcc cgtggtgctc agtacgctcg cgctcaccgt ggccccggca ttctccaagg
     1261 catagcccat ggcggtatac ggctccgtgg tggggatcaa gccgcccgag cgcagcgcgt
     1321 cgctcatcag gttggtgccc caattgatgg gaccaccgag gaataccgtt gccccaaggg
     1381 tgcccgtgtt ccccgtctgg ccggggtcgg tcaggtagag g
//
LOCUS       DAOQOC010000949         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77149_length_1419_cov_2.763930, whole genome shotgun sequence.
ACCESSION   DAOQOC010000949 DAOQOC010000000
VERSION     DAOQOC010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77149_length_1419_cov_2.763930"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1419)
                     /locus_tag="PL106_13390"
     CDS             complement(<1..>1419)
                     /locus_tag="PL106_13390"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNA34114.1"
                     /translation="NLIRTGSIGDSVGLAQMEKWVKPFDGGYRITSVTDADGKKLPYT
                     INKTMMRVDLPKPLAPGATFSFKVKWWNWINDRSKWGGRSGYEYFPDEDNYLFTIAQF
                     YPRMAAYMDHTGWQHKQFLGQGEFTLDFGDYTLSITTPGDHIVAATGEWANASAVLTP
                     VQQKRLALARTSDEPVMIVTPEEALDNEKERSIGTKTWVFKSRNVRDVAFASSRKFIW
                     DAMNQPVGTNNVLCMSYYPKEGNPLWERYSTKVVAHTIRTYSKFTAEYIYPVAISVHT
                     DRIGMEYPMICFNGGRPEKDGTYSERTKYGMIGVIIHEVGHNFFPMIINSDERQWTWM
                     DEGLNTFVQYLTEQEWDKDYPSWRGKPRNIVDYMKGDPDAAPDKVKARIEPIMTNSES
                     ITQFGNNAYGKPATALNILRETVMGRELFDHAFKTYCDRWKFKHPTPADFFRTMEDAS
                     AVDLDWFWRGWFYTTDHVDVELA"
BASE COUNT          227 a          419 c          437 g          336 t
ORIGIN      
        1 gccagctcga cgtccacgtg gtcggtggtg tagaaccagc cgcgccagaa ccagtcgagg
       61 tccacggcgc tggcatcttc catggtgcgg aagaagtcgg ccggggtggg gtgcttgaat
      121 ttccagcggt cgcaataggt cttgaaggcg tggtcgaaga gttcacgccc catcaccgtt
      181 tcgcgcagga tgttcaacgc cgtggccggc ttgccgtacg cgttgttgcc gaactgggtg
      241 atgctctcgc tgttggtcat gatcggctcg atgcgggcct tcaccttgtc cggtgcggca
      301 tcggggtcgc ctttcatgta gtcgacgatg ttgcggggct tgccacgcca gctggggtag
      361 tccttgtccc actcctgctc ggtgaggtat tggacgaagg tgttgaggcc ctcatccatc
      421 catgtccact gccgctcgtc ggagttgatg atcatgggga agaagttgtg gcccacctcg
      481 tggatgatca caccgatcat gccgtacttg gtgcgttcgc tgtacgtgcc gtccttctcg
      541 ggccgcccgc cgttgaagca gatcatgggg tactccatgc cgatgcggtc cgtatgcacg
      601 ctgatggcca ccgggtagat gtactccgcc gtgaacttgc tgtaggtcct gatcgtgtgc
      661 gccaccacct tggtgctgta ccgttcccag agcgggtttc cctcctttgg gtaatagctc
      721 atgcagagca cgttgttcgt ccccaccggt tggttcatcg catcccaaat gaacttccgg
      781 ctgctggcga aggccacgtc gcgcacgttc ctgctcttga acacccaggt cttcgtaccg
      841 atgctccgtt ccttttcgtt gtccaatgct tcctccggcg ttacgatcat caccggttcg
      901 tcgctggtac gggccagggc caggcgcttc tgctgaacgg gagtgagcac cgctgatgca
      961 ttcgcccatt cgccggtggc ggccacgata tgatcaccgg gtgtggtgat gctgagcgtg
     1021 tagtcgccga aatcaagggt gaactcgccc tgccccagga actgcttgtg ctgccatccg
     1081 gtatggtcca tgtaggcggc catgcgcggg tagaactggg ctatggtgaa gaggtagttg
     1141 tcctcatcag gaaaatactc gtagccgctg cgaccgcccc acttcgaacg atcattgatc
     1201 cagttccacc acttcacctt gaagctgaag gtggcgccgg gcgcgagggg cttgggcagg
     1261 tccacgcgca tcatcgtttt gttgatggtg tacgggagtt ttttcccgtc cgcatccgtc
     1321 acggaagtga tcctatagcc gccgtcgaac ggcttcaccc acttctccat ctgggccagg
     1381 cccacgctgt cgccgatgga gcccgtgcgg atgaggttc
//
LOCUS       DAOQOC010000950         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77162_length_1419_cov_2.384897, whole genome shotgun sequence.
ACCESSION   DAOQOC010000950 DAOQOC010000000
VERSION     DAOQOC010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77162_length_1419_cov_2.384897"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PL106_13395"
     CDS             complement(<1..228)
                     /locus_tag="PL106_13395"
                     /inference="COORDINATES: protein motif:HMM:NF020291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HNA34115.1"
                     /translation="MEHREALEGLLKRAHNRLPIVRGSRNTDLYKLAADFNNYGIPKE
                     EALNACARFVDLSGPDPFTAEEIRATVESAYK"
     gene            complement(436..>1419)
                     /locus_tag="PL106_13400"
     CDS             complement(436..>1419)
                     /locus_tag="PL106_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNA34116.1"
                     /translation="QEERDRTKRQEGHEAPKVRPVWRPHLVGDITMEALVGKLECSPR
                     GLGVHRDELAGWWGSMDQYRKGSDRENWLTLHNGSTVDALRRTAGEVRVPLPFVSVCG
                     TVQPAKLHTLGKGQDGFQPRILFAYPEKQTKPYFSDASIGSEWPLSYTTVLDRLLALP
                     LNVENNRPAPRMLEFSETARVAFSAWVNLNTDRANAAPSDGIRDIYPKLETYAARIAL
                     VLELLDHAATGSAVSEVISGKAMKGAIDLVEYFATTALKVHFHLNEEDAVDRLPDLRK
                     RVYEALPDQFRTGEGQEIADKFGMPQRTFKRFIGNENLFRKEAHGVVTKLR"
BASE COUNT          235 a          426 c          439 g          319 t
ORIGIN      
        1 tttgtaggcg ctttccacgg tggcccgtat ctcttcggcc gtgaaggggt cggggccgga
       61 caggtccacg aaacgggcgc atgcgttcag ggcttcctct ttcgggatgc catagttgtt
      121 gaagtcggcg gccaacttgt acaggtcagt gttgcgcgat ccccgaacga tcgggaggcg
      181 gttgtgcgcc cgtttcagca gcccttcgag ggcttcgcgg tgttccatac gtgtggcaca
      241 attggcactt ttggcacttt gcccgtataa cggggctttc cggcggaatt gcgtggcact
      301 ttggtggcac aattggcact ttgcccggcc ggttggcgcc cccattgtgc caattgtgcc
      361 accaaagtgc cacgctttgg cacgctgaaa cccgcgccca gcctacaaag tgccaaaagt
      421 gccaattgtg ccacgtcaac ggagcttggt cactaccccg tgggcctctt ttcggaacag
      481 gttctcgttc ccgatgaagc gcttgaaggt gcgctggggc atgccgaatt tgtccgcgat
      541 ctcttggcct tcccctgtac ggaattggtc gggcagcgct tcatagaccc gtttccgaag
      601 gtccggcaag cggtccaccg catcctcttc gttcaggtgg aagtggacct tcagcgcggt
      661 ggtggcgaag tattccacca gatcaatggc gcctttcatg gccttaccgc tgatgacttc
      721 ggacaccgcg ctaccggttg ccgcatggtc caacagttca aggaccaagg caatgcgggc
      781 cgcataggtt tccagcttgg ggtagatgtc ccgtatccca tcgctggggg ctgcgttggc
      841 gcggtccgtg ttaaggttca cccatgcgct gaaagccacc cgtgcggtct cggaaaattc
      901 cagcatgcgg ggagccggtc ggttgttctc cacgttcagg ggcagggcca gcaaccggtc
      961 cagcactgtg gtataactca acggccattc ggagccgatg gaggcatcgg agaaataggg
     1021 ctttgtttgc ttttcggggt atgcgaagag gatccggggc tggaagccgt cctgcccctt
     1081 gcccaaggtg tggagcttgg cgggttgtac cgtaccgcaa acgctcacga agggtagggg
     1141 cacccgtact tcgcctgccg tgcgacggag ggcatccacc gtgctaccgt tgtgcagggt
     1201 aagccaattt tcgcggtcgc ttcccttgcg gtactggtcc atggatcccc accacccggc
     1261 cagttcgtcc cgatggacgc ccagcccgcg cggggagcat tccagcttgc ccaccaaggc
     1321 ttccatcgta atgtccccga ccaaatgtgg ccgccatacg gggcgcacct tcggggcttc
     1381 gtgcccttcc tgccgcttgg tacggtcgcg ctcttcttg
//
LOCUS       DAOQOC010000951         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77302_length_1418_cov_2.305943, whole genome shotgun sequence.
ACCESSION   DAOQOC010000951 DAOQOC010000000
VERSION     DAOQOC010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77302_length_1418_cov_2.305943"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PL106_13405"
     CDS             <1..765
                     /locus_tag="PL106_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34117.1"
                     /translation="ATPSGQQDLVSVALHEMGHGLGFVGLSEKEGATGSLGLLLASDF
                     APLTTTFPWPELDSLPGIFDRFLVHPQNGPLDLMENPGPTLGSAMSSNQLRFNGPLTM
                     IANDGEAPRIYSPSNFALGSSCVHLNEATYPVGNANELMTPFNSAGHANHWPGPLCLV
                     MLRDIGWNLAPDVGMVEPCARSPLLELWPNPVQSELSISGIIAGANVVIRDVQGRTVM
                     VLQGGSSIDVSGLAPGPYLVSTVAGGLAKHGRLIKR"
     gene            complement(755..1228)
                     /locus_tag="PL106_13410"
     CDS             complement(755..1228)
                     /locus_tag="PL106_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA34118.1"
                     /translation="MGPIRTFFTAFAERDWTLMGACYHDGARFNDRIFHQLEAREVRA
                     MWRLRLNGVEDLRLAFLVLEENAGSGRVRWNAWYTTNGGRRVHDRVTSTFAFKDGLIL
                     RQDDQFDFWRWSRQVLGLKGLLLGWTTWLRDEVRKGAMVRIRKSPELAGPQGTTF"
     gene            1227..>1418
                     /locus_tag="PL106_13415"
     CDS             1227..>1418
                     /locus_tag="PL106_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34119.1"
                     /translation="MVRMLPSCTASPGKCDGGRRIHIAPGHAGGLFPTFAPMSRHHYT
                     LLLEWTGNAGTGTDHYRSYK"
BASE COUNT          273 a          477 c          405 g          263 t
ORIGIN      
        1 gccacaccat ccggccagca ggacctggtg agcgtcgcac tgcacgagat ggggcatggt
       61 ctcggtttcg tggggctcag cgagaaggag ggagccaccg gctccctggg gctgctgctc
      121 gcctcggact tcgcaccgct gaccaccaca ttcccttggc cggaactcga ttcgctgccc
      181 gggatcttcg accgttttct cgttcacccg cagaacggtc cgttggacct gatggagaat
      241 cccggtccaa cgcttggatc cgcaatgtcg agcaaccaac tgcggttcaa cggtccactg
      301 acgatgatcg ccaacgacgg cgaggcccca aggatatact ccccttccaa cttcgcactg
      361 ggcagcagct gtgtccactt gaatgaagcc acctatcccg tgggcaacgc gaacgaactg
      421 atgacgccgt tcaattccgc cggtcatgcc aaccactggc cggggccact gtgcctggtc
      481 atgctgcggg atatcggctg gaacctcgct ccggatgtag gcatggtcga accttgcgca
      541 cgttcgcctc tgcttgagct gtggccgaac cccgtacaga gcgagctctc catcagcggc
      601 atcatcgccg gggccaacgt ggtgatccgt gacgttcagg gccgcaccgt gatggtcctg
      661 caagggggat catccatcga cgtctccggg ctggctcccg gcccctacct cgtatccacg
      721 gtggcaggag gcctggcgaa acatggacgc ttgatcaaaa ggtagttccc tgtggcccgg
      781 ccagctcggg agacttccgg atgcgcacca tcgctccttt gcgcacctcg tcgcgcaacc
      841 aggtggtcca gcccagcaga agccctttga ggcccagtac ctggcggctc caacgccaga
      901 agtcaaattg atcatcctgc cgcaggatca gtccgtcctt gaacgcgaag gtggacgtta
      961 cacggtcgtg aacccgtctg cccccgttcg tggtgtacca tgcattccaa cggacacgac
     1021 cactgcccgc gttctcttcc agaacaagaa aggccagccg caggtcctcg accccgttca
     1081 ggcgcaacct ccacatcgca cgcacctccc tggcctccaa ttggtggaag atccgatcat
     1141 tgaagcgcgc accatcgtgg tagcacgcac ccatcaacgt ccagtcacgc tcggcgaagg
     1201 cggtgaaaaa tgtacggatg gggcccatgg tgcggatgct cccctcttgt acggcaagcc
     1261 cgggaaaatg tgacggtggt cgtcggatcc atatcgctcc cggccatgct ggcggcctct
     1321 tcccgacctt cgcccccatg tcaaggcatc actacacgct cctgctcgaa tggacgggga
     1381 acgcgggcac cggcacggat cattaccgca gctacaag
//
LOCUS       DAOQOC010000952         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77361_length_1417_cov_3.556535, whole genome shotgun sequence.
ACCESSION   DAOQOC010000952 DAOQOC010000000
VERSION     DAOQOC010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77361_length_1417_cov_3.556535"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1417
                     /locus_tag="PL106_13420"
     CDS             21..>1417
                     /locus_tag="PL106_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNA34120.1"
                     /translation="MGIADMDGDGLDDLILLQQSKTLSIDYQNADGSFTSYAYGNVSN
                     SSQWGMAVGDVDNDGHKDLVSGGNGDGVHFVEISGRGVYTPVTNLNNGSMFMQCMNMM
                     DMNNDGWLDVFGCHDNAAPRTWLNNGSGSLTYNAWINYATSPSSDMSGNYGSTWTDFD
                     NDGDVDLYITKCRQGVNNSSDPRRWNRLFVNDGSNNYTDQALAYGVQNREQSWSSDFA
                     DIDNDGDLDLITTNHSTTIQLFINDGTGHYTNATSGSGLEYSNFFLQTKCADLDNDTY
                     IDLLVTEGNYYFHNNGNGTFTRITNLLPHPSQSYTLHSFAMGDMNHDGAIDIYASYGT
                     GYVDPSGSRDDELYLNNGNSNHWIDFELEGTESNRDAVGARVTLYGAWGVQMREVRAG
                     ESYGIVNSFNCHFGLGTYTEVDSAIVRWPSGTTDRYYNLAADQWVVVNEGWTTRAKMS
                     LVAALAGPYVQGTGL"
BASE COUNT          334 a          406 c          441 g          236 t
ORIGIN      
        1 ccgcacgttc gggtggttgt gtgggcatcg cggacatgga cggtgatggc ctggatgact
       61 tgatcctcct gcaacagagc aagaccttga gcatcgatta ccagaacgcc gacggcagct
      121 tcacgtcata cgcgtacggc aacgtttcca atagcagcca gtggggcatg gccgtgggcg
      181 atgtggacaa cgacgggcac aaggacctcg tgagcggagg caacggggac ggggtccact
      241 tcgtggagat cagcggccgt ggggtgtaca cgcccgtcac caacctgaac aacggaagca
      301 tgttcatgca gtgcatgaac atgatggaca tgaacaatga tggctggctt gacgtgttcg
      361 gatgccacga caacgccgcc ccgcgcacgt ggttgaacaa cggcagtggt tcgctcacct
      421 acaacgcctg gatcaactac gccacctcgc ccagcagcga catgagcggc aactacggca
      481 gcacctggac ggacttcgac aacgatgggg atgtggacct gtacatcacg aagtgcaggc
      541 agggcgtgaa caattcgagc gatccgcgcc gatggaaccg gttgttcgtg aatgacggga
      601 gcaacaatta taccgaccag gccctggcgt acggtgttca gaaccgggag cagagctgga
      661 gcagtgactt cgccgacatc gacaacgacg gggacctgga cctgatcacc acgaaccaca
      721 gcaccacaat ccagttgttc atcaatgacg ggaccgggca ctacaccaat gccaccagcg
      781 ggagcggcct ggagtacagc aactttttcc tgcagacgaa gtgtgccgac ctggacaacg
      841 acacgtacat cgatctgctg gtgacagagg gcaattacta cttccacaac aacgggaacg
      901 gcaccttcac ccggatcacc aacctgctcc cgcacccgtc gcagagctat acactccaca
      961 gcttcgccat gggcgacatg aaccacgacg gggccatcga catctacgcc agttatggaa
     1021 cgggttatgt ggacccgagc ggatcacggg acgatgaatt gtacctgaac aacggcaact
     1081 caaaccattg gatcgacttc gagctggaag ggacggagag caaccgcgat gcggtgggtg
     1141 cgcgggtgac cctgtacggc gcatggggcg tgcagatgcg cgaggtgcgg gccggggaga
     1201 gctacggcat cgtgaacagt ttcaactgcc acttcggtct gggtacgtac actgaggtgg
     1261 acagtgccat tgtgcgttgg cccagcggca ccacggaccg ctactacaac ctggcggccg
     1321 atcaatgggt ggtggtgaac gaaggttgga ccacccgggc gaaaatgagc ctggtggccg
     1381 cactggccgg gccctacgtc caaggtaccg gtctcat
//
LOCUS       DAOQOC010000953         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77368_length_1417_cov_3.345081, whole genome shotgun sequence.
ACCESSION   DAOQOC010000953 DAOQOC010000000
VERSION     DAOQOC010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77368_length_1417_cov_3.345081"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1417
                     /locus_tag="PL106_13425"
     CDS             <1..>1417
                     /locus_tag="PL106_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA34121.1"
                     /translation="LLVSSFAIRAQVPIAIPPALDLDTFHLVVDEHVHQFYPGVNTNT
                     YGVGVPYLGPTLIFHTGDTARIRVQNDLEQVTSMHWHGLRVPAEFDGGPPREIAPGDH
                     WDVKYQVKNPAGTYWYHPHPHELTAEQASLGVAGFILVQDDAEAALDLPRTYGVDDFP
                     IVVQDRRFQPNGDFVFGPFGDSVLVNGTPHPFLECPAQVVRLRLLNGSNARVYALGFE
                     SDEPFSVIAGDGGLLDAPVTTNRLPLSNGERAEILLDLTGMEGDSLLLMSYGSELPAT
                     VPGSSYILWESSALNGIDFPVLRIRVGSPTIDPVTTFPASLVPVDAYDEADATNTRTK
                     VLSGTGMVGMGMFYINGGMFDMDVINDTIMLGATEIWEIQNNSNMAHPMHIHGGSCYV
                     LDRGGDEPPAWEQGAKDVVLVDAGETVRVIMKFEDLTDGWPFMYHCHNLMHEDNMMML
                     QYIVVDPSTAIPAGKSTEGVTV"
BASE COUNT          279 a          433 c          424 g          281 t
ORIGIN      
        1 gctcctggtc tcctctttcg cgatccgtgc ccaggttccg atcgccattc ctccggcact
       61 ggacctggac acgttccacc tggtcgtgga cgagcatgtc caccagttct accccggggt
      121 gaacaccaat acctacggcg tgggtgttcc gtacctcgga cccacactca ttttccatac
      181 cggggacacc gcgcggatcc gcgttcagaa tgatctggaa caggtcacca gcatgcactg
      241 gcatgggttg cgcgtacccg ctgaattcga tggcgggcca cctcgcgaga tcgctcccgg
      301 cgatcactgg gacgtgaagt accaagtgaa gaatccggca ggtacctatt ggtatcatcc
      361 gcacccgcac gaactcaccg ccgaacaagc cagtcttggc gtggcgggct tcattctcgt
      421 gcaggacgat gcggaggccg cactcgacct gccgcgcacg tacggggtcg atgatttccc
      481 gatcgtggtc caggatcgca ggttccaacc caacggcgat ttcgttttcg gccccttcgg
      541 tgattcggtg ctggtcaatg gcacgccgca tcctttcctg gaatgcccgg cccaagtggt
      601 gcgcctacgc ctgctgaacg gatcgaatgc gcgcgtttac gccctgggat tcgagagcga
      661 tgagccgttc tccgttatcg ccggagatgg cggcctgctc gatgcgccgg tgaccacgaa
      721 caggttgcca ttgagcaacg gcgaacgcgc cgagatcctc ctggacctca ccgggatgga
      781 aggcgacagc ctgttgctga tgagttacgg gtcggaactt cccgccacgg tgcccggcag
      841 ttcatatatc ctctgggaaa gcagtgcgtt gaacgggatc gacttccccg tgctgcgcat
      901 ccgcgtggga tcaccaacga tcgacccggt caccacattc ccggcctctc tcgtcccggt
      961 tgatgcctac gacgaggccg acgccaccaa cacgcgcacc aaggtgctca gcggtaccgg
     1021 tatggtgggc atgggtatgt tctacatcaa tggtggaatg ttcgatatgg acgtgatcaa
     1081 tgatacgatc atgctgggtg caacggagat ctgggagatc cagaacaact ccaacatggc
     1141 ccaccccatg cacatccacg gtggatcgtg ctacgtgctg gaccgcggcg gtgatgagcc
     1201 ccccgcgtgg gaacaaggag ccaaggacgt ggtgctcgtt gatgcgggcg aaacggtgcg
     1261 ggtgatcatg aaattcgagg accttaccga tggctggcca ttcatgtacc attgccacaa
     1321 cctgatgcac gaggacaaca tgatgatgtt gcagtacatc gtggtggatc cgagcaccgc
     1381 catcccggca gggaaatcga cggagggcgt caccgtg
//
LOCUS       DAOQOC010000954         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77373_length_1417_cov_3.234949, whole genome shotgun sequence.
ACCESSION   DAOQOC010000954 DAOQOC010000000
VERSION     DAOQOC010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77373_length_1417_cov_3.234949"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PL106_13430"
     CDS             complement(<1..511)
                     /locus_tag="PL106_13430"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNA34122.1"
                     /translation="MRDLFRFLLRQRDNLLFLLLLVLGLSMLIRGNMHQRAEWVSTSN
                     AVTGRVFAFRSGIMAYTGLRESNIALNEALARERERSHAVIERRDTGAVRMDTIRHLR
                     YRYRTAMVINSTTHKAKNYITLDKGTLDGVERNMGVIGTNGIVGVVREASAHFASVIS
                     VLSEDHVASA"
     gene            complement(593..>1417)
                     /locus_tag="PL106_13435"
     CDS             complement(593..>1417)
                     /locus_tag="PL106_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNA34123.1"
                     /translation="PLRDGVIADFQAAEEMIKGMIRMINPGRRLFTPNLRMVICIPSG
                     ITEVEKRAVKDSSEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDIGGGTSEIAV
                     IALGGIVCDKNIRVAGDEFTQDIEEYMRRQHNILVGERTAELIKIEVGSAMTELDNPP
                     PDYSVRGRDLMTGIPKEITVTYSEIAQALDKSISKIEEAILSALESTPPELSADIYKT
                     GIYLAGGGALLRGLDKRISVKTKLPVHVSEDPLRAVARGTGIALKNIDRFQFLMRE"
BASE COUNT          226 a          440 c          452 g          299 t
ORIGIN      
        1 gtgcgctggc cacatggtcc tcgctcagta cgctgatcac cgatgcgaag tgcgcactgg
       61 cctcgcgcac cacgccaacg atgccgttgg tgccgatcac gcccatgttg cgttccaccc
      121 cgtccagcgt ccccttgtcg agggtgatgt agttcttggc cttgtgggtg gtgctgttga
      181 tcaccattgc ggtacgataa cgatacctta ggtgtcggat cgtatccatc cgtaccgcac
      241 cggtgtcgcg tcgttcgatc acggcatggg aacgctcgcg ctcacgggcg agtgcctcat
      301 tgagggcgat gttgctctcg cgcagaccgg tataggccat gataccgctg cgaaaggcga
      361 aaacgcggcc ggtcacggca ttgctggtgc tcacccattc ggcacgctgg tgcatgttgc
      421 ccctgatcag catggagagg cccaggacca gcaggagcag gaacagcagg ttgtcgcgct
      481 gacggaggag gaagcggaag aggtcgcgca tctcgtgagg gttgcgagag gcgagtggcg
      541 gatggcgagt ggcctggtgc tgaagcgccc gccacccgcc atccaccgct catcactccc
      601 gcatcaggaa ctggaagcgg tcgatgttct tcagggcgat gccggtacca cgcgccacgg
      661 cccgcaacgg gtcctcgctc acgtgtacgg gaagcttggt cttcacgctg atgcgcttgt
      721 ccagtccgcg caacagggca ccgccacccg ccagatagat accggtcttg tagatgtcgg
      781 cactcagttc cgggggtgtg ctctcgagcg cgctgaggat ggcctcctcg atcttgctga
      841 tcgatttgtc cagtgcctgg gcgatctcgc tgtaggtgac ggtgatctcc ttggggatgc
      901 cggtcatcag gtcgcggccg cgaacactgt agtccggcgg cgggttgtcg agctcggtca
      961 tggcgctgcc cacctcgatc ttgatcaact cggccgtgcg ttcgcccacc aggatgttgt
     1021 gctggcgccg catgtactcc tcgatgtcct gggtgaattc atcccctgcc acgcgtatgt
     1081 tcttgtcgca cacgataccg ccgagggcga tcaccgcgat ctcgctggtt ccgccgccga
     1141 tgtcgatgat catgttcccc atgggttcct ccacatcgat accgatgccg atcgcggcgg
     1201 ccatgggctc gtggatcagg tacacctcct tggccccggc gtgctcgctg ctgtccttca
     1261 ccgcgcgttt ctccacctcg gtgatgccgc tggggatgca gatcaccatg cgcaggttgg
     1321 gtgtgaagag gcgacggccg gggttgatca tgcggatcat gcccttgatc atctcctcgg
     1381 ccgcttggaa atcggcgatc acaccgtcgc gcagggg
//
LOCUS       DAOQOC010000955         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77422_length_1417_cov_2.302496, whole genome shotgun sequence.
ACCESSION   DAOQOC010000955 DAOQOC010000000
VERSION     DAOQOC010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77422_length_1417_cov_2.302496"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PL106_13440"
     CDS             complement(<1..487)
                     /locus_tag="PL106_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34124.1"
                     /translation="MAEIVRMPKLSDTMTEGVVAKWSKKVGDKVKSGDILAEIETDKA
                     TMEFESYTDGVLLHIGVEQGKAAPVDSVLAVLGAAGEDITALLAEAAKTMPAPVAAPS
                     PKKVEVPKAAPPAAPVPVAPAASTKSVPVAVVANGAAPAHAAGDKRVKASPLAKHLAE
                     DV"
     gene            complement(548..>1417)
                     /gene="pdhA"
                     /locus_tag="PL106_13445"
     CDS             complement(548..>1417)
                     /gene="pdhA"
                     /locus_tag="PL106_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNA34125.1"
                     /translation="LYIGQEAVLAGMITALRPTDRIITGYRDHAHPLVLGESPGRVMA
                     ELYARATGTSKGKGGSMHYFDKERNLFGGHGIVGGQIGLGAGIAFADKYRGEDHATLC
                     MFGDGASRQGILHETFNMAMTWKLPVIFIIENNNYAMGTSVERTSNVHDLSTLGESYD
                     MPGVVVDGMSCEAVHGAIVDACAHVRAGKGPILLDIRTYRYRGHSMSDPQKYRTKEEV
                     EEYKKQDPIENVRSVLLEKKWATAEELEAMDTAAKKEVEEAVKFAEESPSPATEELWN
                     DVYKEPDYPFIKD"
BASE COUNT          194 a          490 c          428 g          305 t
ORIGIN      
        1 gcacgtcctc ggcgaggtgc ttcgcgagcg ggctggcttt cacgcgtttg tcgcccgcgg
       61 catgcgccgg ggccgcgccg ttggccacca ccgcaaccgg aacggacttg gtggatgccg
      121 ccggtgcaac gggtacagga gcggcaggag gtgcggcttt gggtacctcc accttcttcg
      181 gggaaggcgc cgctacaggg gcgggcatgg tcttcgcggc ttcggccagc aacgccgtga
      241 tgtcctcccc ggctgcgccc agtacggcca gcacactgtc caccggtgcg gccttgccct
      301 gttccacgcc gatgtgcaac agcaccccat cggtgtagct ctcgaactcc atggtggcct
      361 tgtccgtctc gatctcggcc aggatgtccc cgctcttcac cttgtcgccc acttttttgg
      421 accacttcgc caccacgccc tcggtcatgg tgtcgctcag cttcggcata cgtacgatct
      481 cggccatcgt gtgtctgttg ttctgttcca ggggtacagc ctgccgcaac gccctgatgg
      541 ttggtcctca gtccttgatg aaggggtagt ccggctcctt gtacacgtcg ttccacagtt
      601 cctcggtggc cggtgaggga ctctcctccg cgaacttcac cgcttcctcc acctctttct
      661 tcgccgcggt gtccatggcc tccagctcct cggcggtggc ccacttcttc tccagcagca
      721 cggatcgcac gttctcgatg gggtcctgct tcttgtactc ctcgacctcc tccttggtgc
      781 ggtatttctg cgggtcgctc atgctgtgcc cacgataccg gtatgtgcgt atgtccagca
      841 ggatgggccc cttgcccgca cgcacgtggg cgcaggcatc gacgatggca ccgtgcacgg
      901 cctcgcagct catcccgtcc accaccacgc cgggcatatc gtagctctcc cccaaggtgc
      961 tgaggtcatg cacgttgctg gtgcgctcca cgctggtgcc catggcgtag ttgttgttct
     1021 cgatgatgaa gatcaccgga agcttccacg tcatggccat gttgaaggtc tcgtgcagga
     1081 tgccctggcg cgaggccccg tcgccgaaca tgcacaacgt ggcgtggtcc tcgccgcggt
     1141 acttgtcggc gaaggcgata ccggcaccca ggccgatctg cccgcccacg atgccgtggc
     1201 caccgaacag gttgcgctcc ttgtcgaagt agtgcatgct gccgcccttg cctttgctgg
     1261 tgccggtggc ccgcgcgtac agttcggcca tcacgcgccc gggcgactcg cccaacacga
     1321 gcggatgcgc atgatcccgg tatccggtga tgatccggtc cgtcggtcgg agggccgtga
     1381 tcatgcccgc cagcacggcc tcctgaccga tgtacag
//
LOCUS       DAOQOC010000956         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77596_length_1415_cov_3.430882, whole genome shotgun sequence.
ACCESSION   DAOQOC010000956 DAOQOC010000000
VERSION     DAOQOC010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77596_length_1415_cov_3.430882"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..347
                     /locus_tag="PL106_13450"
     CDS             96..347
                     /locus_tag="PL106_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34126.1"
                     /translation="MQHPIPGRHCCGTTLRPNGARGSGSSSFSVLAQVAQQYHFADPR
                     EPTINWLHAGIHTSNTIHAFNIGRSIQGPDEGSSADDPR"
     gene            complement(440..>1415)
                     /gene="groEL"
                     /locus_tag="PL106_13455"
     CDS             complement(440..>1415)
                     /gene="groEL"
                     /locus_tag="PL106_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNA34127.1"
                     /translation="IYDKKISTMKELLPVLEKAAQAGKPLLIISEDVDGEALATLVVN
                     KIRGALKVAAVKAPGFGDRRKAMLEDIAILTGGTVISEERGFKLENADLSMLGKAEKI
                     NIDKDNTTIVNGAGKKADITGRVNQIKAQIETTTSDYDKEKLQERLAKLAGGVAVLYI
                     GAATEVEMKEKKDRVDDALHATRAAVEEGIVPGGGVAYIRAQKALEKMEGANNDETTG
                     MGIVRRALEEPLRQIVANAGLEGSIVVQKVREGKADYGFNARTEEYENLLAAGVIDPT
                     KVTRVALENAASIASMLLTTECVISEEKEEKAAGGHAHPGMGGGMDMM"
BASE COUNT          212 a          470 c          418 g          315 t
ORIGIN      
        1 ggtgcctcca ttgcgggcaa atgttgtccg gtgttccaac cgccgcaata ccggaaaacc
       61 atgaactggt gggcaagcga tgatgacacg gtcacatgca acacccgata cccgggaggc
      121 attgctgcgg gactactctc cggcccaacg gagcgcgcgg ttccggctcc agcagcttca
      181 gcgtactcgc gcaagtggcc cagcagtacc attttgccga tccacgcgag cccaccatca
      241 actggctcca tgcgggcatc cataccagta ataccatcca cgcattcaat atcggccgat
      301 ccatacaggg tccggatgaa ggttcatcgg cggatgatcc ccggtgaact ccttcttctc
      361 ctccccctga acgggacaag ccccggactt ccgcagtgct gcggtagccg gggcttgttc
      421 tggtcggttg ttcggacgct tacatcatgt ccatgccgcc acccatgccg gggtgcgcat
      481 gtccaccagc ggccttctct tccttctcct cgctgatcac gcactcggtg gtgagcagca
      541 tgctggcgat ggaagcggcg ttctccaggg caacgcgggt caccttggtg gggtcgatca
      601 caccggcggc gagcaggttc tcgtactcct cggtgcgggc gttgaagccg tagtcggcct
      661 tgccctcacg caccttctgc accacgatgc tgccttcgag gccggcgttg gccacgatct
      721 ggcgcagggg ctcttcgagc gcacggcgca cgatgcccat gcccgtggtc tcgtcgttgt
      781 tggcgccctc cattttctcc agcgctttct gcgcgcggat gtaggccaca ccaccgccgg
      841 gcacgatgcc ctcttctacc gcagcgcggg tagcgtgcag cgcgtcgtcc acgcggtcct
      901 tcttctcctt catttccacc tcagtggcgg caccgatgta gagtactgcg acgccaccgg
      961 ccagcttggc caggcgctcc tgcagcttct ccttgtcgta gtcgctggtg gtggtctcga
     1021 tctgggcctt gatctggttc acacgaccgg tgatgtcggc cttcttgccg gcaccgttca
     1081 cgatggtggt gttgtccttg tcgatgttga tcttttcggc cttgcccagc atgctcaggt
     1141 ccgcgttctc cagtttgaag ccgcgctcct cgctgatcac ggtgccaccg gtgaggatgg
     1201 cgatgtcctc cagcatggcc ttgcggcggt cgccgaagcc cggggccttc acggcggcca
     1261 ctttcagggc gccacggatc ttgttcacca ccagggtggc gagcgcttca ccgtccacgt
     1321 cctcgctgat gatcagcagg ggcttgccgg cctgggcggc tttttcgagc acggggagca
     1381 actccttcat ggtgctgatc ttcttgtcgt agatc
//
LOCUS       DAOQOC010000957         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77722_length_1414_cov_2.822664, whole genome shotgun sequence.
ACCESSION   DAOQOC010000957 DAOQOC010000000
VERSION     DAOQOC010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77722_length_1414_cov_2.822664"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PL106_13460"
     CDS             <1..294
                     /locus_tag="PL106_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNA34128.1"
                     /translation="ANQGENRLADSRDVKRIHDTELAVITDEEKRFDRLVELNVAEQV
                     YALSRTAVIREAWEQGRKIHVHGWVYGLSDGLIKDLKVTRTRYELEIGGVDLR"
     gene            358..>1414
                     /locus_tag="PL106_13465"
     CDS             358..>1414
                     /locus_tag="PL106_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA34129.1"
                     /translation="MSNATFTAPAPKNEPVLSYAPNSPERAALQAEVDRRMRTRIDAP
                     MWIAGHAITTKDLRKMSPPHKHAHNLGSAHFGEAKHVRAAIDAAMKAKRAWENTPFAD
                     RAAIFLKAADLIAGPYRQQINAATMLGQGKNCFQAEIDSACELIDFLRFNVTYAEQIY
                     RDQPVSSPGVWNRLEYRALEGFVFALTPFNFTAIAGNLPSSCALMGNTVVWKCADTQC
                     YSAQVLMEVFMAAGLPAGVINLIHVEGPVAGDVIFKHKDFAGIHFTGSTKVFRHIWQE
                     IGNNLPMYRSYPRIVGETGGKDFVIAHPSADPKVVATALSRGAFEYQGQKCSAASRAY
                     IPANLWPDVKKELLADVN"
BASE COUNT          295 a          437 c          453 g          229 t
ORIGIN      
        1 gccaaccaag gtgagaaccg ccttgcggat agccgcgacg tcaagcggat ccacgatacg
       61 gagctggcgg tcatcactga tgaggagaag cgctttgacc ggttggtgga gctgaatgtg
      121 gccgaacagg tctatgccct gagccggaca gcggtcatac gggaagcgtg ggagcagggg
      181 cgcaagatcc atgtacatgg ctgggtatac ggcctgagtg atggcctgat caaggacctg
      241 aaggtgacgc gtacccggta cgaactggag atcggggggg tggacctgcg ctgaaccgac
      301 cgggtgcatg cttcatgggg gctggtatct tcgccgtcac ttcccgaccc gatccccatg
      361 agcaacgcga ccttcacagc tccagcgccg aagaacgaac ccgtcctcag ttacgccccg
      421 aactcacccg aaagggccgc cttgcaagcc gaggtggatc gccggatgcg cacgcgcatt
      481 gatgcgccca tgtggatcgc cgggcatgcg atcacgacca aggacctgcg caagatgagt
      541 ccgccgcaca agcacgcgca caaccttggt tccgcgcatt tcggcgaggc caagcacgtg
      601 cgggccgcca ttgatgcggc gatgaaggcc aagcgggcat gggagaacac gccattcgcc
      661 gaccgcgcag cgatcttcct gaaggcagcg gacctgatcg cgggcccgta ccgccagcag
      721 atcaacgcgg ccaccatgct gggccaggga aagaactgtt tccaggccga gatcgacagc
      781 gcgtgtgaac tcatcgactt cctgcgcttc aacgtgacgt acgccgagca gatctaccgc
      841 gatcagcctg tcagttcacc gggcgtgtgg aaccggctgg aataccgcgc gctggaaggc
      901 ttcgtcttcg cgctcacgcc gttcaatttc accgccatcg ccggtaacct gcccagcagc
      961 tgtgccttga tgggcaacac cgtggtgtgg aaatgcgccg acacgcaatg ctacagcgcg
     1021 caagtgctca tggaggtctt catggcggcc gggctgcccg ccggcgtgat caacctgatc
     1081 catgtggaag gcccggtggc cggggatgtc atcttcaagc acaaggactt cgcggggatc
     1141 catttcaccg gaagtaccaa ggtattccgc cacatctggc aggagatcgg caacaacttg
     1201 ccgatgtacc gcagctaccc gcgcatcgtg ggcgaaacgg gcggcaagga cttcgtgatc
     1261 gcgcacccga gcgccgaccc caaggtggtg gccacggcac tgagccgcgg ggccttcgaa
     1321 taccagggcc agaagtgcag tgccgccagc cgcgcgtaca tccccgccaa cctgtggccc
     1381 gatgtgaaga aagagttgct ggccgatgtg aacg
//
LOCUS       DAOQOC010000958         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77808_length_1413_cov_3.555965, whole genome shotgun sequence.
ACCESSION   DAOQOC010000958 DAOQOC010000000
VERSION     DAOQOC010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77808_length_1413_cov_3.555965"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PL106_13470"
     CDS             complement(<1..464)
                     /locus_tag="PL106_13470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNA34130.1"
                     /translation="MDNAFIATNLDWDHRTPTNGQRFVNRVLRKLGMHVRLSPAPSDM
                     ANVEARMNLFHLLEQTVAFGIPGDVVDLGCNAGDSSVVMQTVIQGLAPEKQVHAFDSF
                     EGLPELTENDRGDGVYSKGHMAAPIDQFKGKFEALGLTLPHIHKGWFEQTVPS"
     gene            634..>1413
                     /locus_tag="PL106_13475"
     CDS             634..>1413
                     /locus_tag="PL106_13475"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HNA34131.1"
                     /translation="MDYISPLDDDTLPGIERRRDVTPQELLEEYVMKSRPVVLTDTVK
                     DWPAMKKWTPQYFKERYGAIKHEVKGKVYTIAEQVELMMHSTMEHPAPYPYNLNVDHV
                     FPELMEDMRPPTVFDRVDRLLSPMMPKLLAKGTIKHELFLSGKGSYFPILHVDVQHLH
                     TQITQVYGSKVFFLYAPDQTPYMYPKPEVPLYSAVDNIFKPDLEKYPLFAKAKCHKLT
                     LHAGDTVFFPTGWWHTTLSPEPSISYGRALLDRTNWNGYLKD"
BASE COUNT          321 a          428 c          395 g          269 t
ORIGIN      
        1 gatggcacgg tctgctcgaa ccagcccttg tggatgtgtg gcaaggtcag ccccagcgcc
       61 tcgaacttcc ccttgaactg gtcgatgggc gcggccatgt ggcctttgga gtacaccccg
      121 tccccccgat cgttctcggt gagttcgggc agtccctcga agctatcgaa ggcatgcacc
      181 tgcttctctg gcgccaggcc ctgtatcacg gtctgcatca ccaccgagct gtccccggca
      241 ttgcagccca gatccaccac atcaccggga ataccgaagg ccacggtctg ctccagcaag
      301 tgaaaaagat tcatgcgcgc ctccacgttg gccatgtccg aaggtgccgg cgacaggcga
      361 acatgcatgc ccagtttccg gagcacgcgg ttcacgaaac gctggccatt cgtgggggtc
      421 cggtggtccc aatcgaggtt ggtggcaata aaagcgttgt ccattgtggt ggatagaggt
      481 ccttggaacg gtagaacgat cccaaagatg cgatcggacg acaagtagcg agctgcgatc
      541 aggatcggac gttaacaacc cgtgttgggt aacttacctt cgtgggtgcc cgttcgagtt
      601 cgttctacac ggaaatcctt aaaccccgga tcgatggact acatctcccc cctcgatgac
      661 gatacactgc caggcataga acgtcgcagg gacgtcacgc cgcaggaact gctggaggaa
      721 tatgtgatga agagcagacc ggtggtgctc acggacaccg tgaaggactg gccggcgatg
      781 aagaaatgga caccgcagta tttcaaggaa cggtacggag cgataaagca cgaggtgaag
      841 ggcaaggtgt acaccatcgc cgagcaagtg gaattgatga tgcattccac gatggaacat
      901 cccgccccgt acccgtacaa cctgaatgtt gatcatgttt tcccggaatt gatggaggat
      961 atgcgtccac ccacggtgtt cgacagggtg gacaggctgt tgagccccat gatgccgaag
     1021 ctcctcgcca aggggaccat caagcacgaa ctcttcctca gcggaaaagg atcctacttc
     1081 cccatcctgc acgtcgacgt gcagcatctc cacacccaga tcacacaggt gtacggcagc
     1141 aaggtgttct tcctgtacgc accggaccag acgccctata tgtaccccaa accggaggtg
     1201 ccgctgtatt ccgcggtgga caacattttc aagcccgatc tggaaaaata cccgctcttc
     1261 gccaaggcca agtgccacaa gctgaccctt cacgccgggg acacggtctt cttccccacg
     1321 ggctggtggc acaccacgct cagccccgaa cccagcatct cctatggccg ggccttgctg
     1381 gaccgcacca actggaacgg ctacctgaag gac
//
LOCUS       DAOQOC010000959         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_77853_length_1413_cov_2.680412, whole genome shotgun sequence.
ACCESSION   DAOQOC010000959 DAOQOC010000000
VERSION     DAOQOC010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77853_length_1413_cov_2.680412"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PL106_13480"
     CDS             complement(<1..186)
                     /locus_tag="PL106_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34132.1"
                     /translation="MKKPGILVLIDWYAPGFKAGGPVRSMVNMIDHLCDQVAFHVVTT
                     DTDYTGTSPYPGITSDAW"
     gene            complement(183..1307)
                     /locus_tag="PL106_13485"
     CDS             complement(183..1307)
                     /locus_tag="PL106_13485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA34133.1"
                     /translation="MSSAPHPTIDQIPGPLDQPARFLFLYTELAPYVIACLRALAERG
                     AEVHVVRWPVNREAPFRMEVDERIHLYERNTMGTDGLLQLAERIKPAVVLASGWVDKG
                     YLAVCRTLRTAGVPTVMCSDTAWRGSARQWLAVGAGRFWIRSAFSHAWVTGTAQADYA
                     RKLGFADSDIRTGFYSADTDLFAPLATSLGAQRALAWPHRFLCTARYIPTKGHQYLCD
                     AFAELCDADEAEDWELWIVGTGELHEQVMRSPSGRHPRIRHLGFIQAEALPAIMADCG
                     VFILPSTYEPWGVVVQENACAGFPLLLSDAVGAAERFLHEGENGHLFRAGDRSSIKQA
                     MLRMVHANDDRLRAMGLRSASIGISWNPDQWAAVAMGLIR"
BASE COUNT          267 a          448 c          444 g          254 t
ORIGIN      
        1 ccatgcgtcc gatgtgatgc ccgggtacgg ggaagtcccc gtgtagtccg tgtccgtggt
       61 cacgacgtgg aatgcgacct gatcacacag gtgatcgatc atgttcacca tgctccggac
      121 aggcccacca gctttgaacc ccggtgcgta ccagtcgatg aggacgagga tcccgggctt
      181 cttcatcgga taagtcccat tgcgacagcg gcccattggt ccgggttcca agagatgccg
      241 atggacgcgc tgcgcaagcc catggcgcgc aaccggtcgt cgttcgcatg caccatgcgc
      301 agcatggcct gcttgatcga cgaccggtcg ccggcccgga acagatgtcc gttctcccct
      361 tcgtgcagga atcgctcggc cgcgccaacg gcatcgctga gcaggagcgg gaacccggcg
      421 caggcgttct cctgcaccac cacgccccac ggttcgtaag tgctgggcag gatgaagacg
      481 ccacaatcgg ccatgatcgc tggcagcgcc tccgcttgaa tgaagccgag atgacggatg
      541 cgcggatgac ggccgctcgg ggaacgcatc acctgctcgt ggagttcacc cgtgccgacg
      601 atccagagtt cccagtcctc cgcttcatcc gcatcgcaca gttcggcgaa cgcatcgcag
      661 aggtattgat gccccttggt ggggatgtat cgtgccgtac acaggaagcg gtgcggccat
      721 gccagcgcac gttgtgcacc gaggctggtc gccagcggtg cgaagagatc cgtgtcggcg
      781 gaatagaagc cggtacggat gtcgctatcg gcgaaaccca gcttgcgcgc gtagtcggct
      841 tgggccgtgc cggtgaccca tgcgtgcgag aaggcactgc gtatccagaa gcgcccggca
      901 cccacggcca gccattgtcg ggcactgcca cgccatgccg tatcgctgca catcaccgtg
      961 ggcacaccgg ccgtgcgcaa cgtacggcac acggcgaggt agcccttgtc cacccagcca
     1021 ctggccagca caacggcagg cttgatccgc tccgcgagtt gcaagagccc atctgtcccc
     1081 attgtgttgc gttcgtacaa atggatgcgt tcgtccactt ccatccggaa aggagcttca
     1141 cggttcaccg gccaacgcac cacgtgtact tcggcaccac gttcagccaa tgcccgcaag
     1201 cacgcgatca cgtacggtgc cagttcagtg tacaggaaca ggaagcgcgc aggctggtcc
     1261 aatggccctg ggatctgatc gatggtcggg tgcggggccg aactcatggc tggccaccgc
     1321 gaatggaggt cagtagcgcc tcgaaggtgt tgaagcggaa gtccttgaac aaggcgtcca
     1381 ttttgcgttc cgtggtgtgc agccccgtgt agg
//
LOCUS       DAOQOC010000960         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78053_length_1411_cov_3.072271, whole genome shotgun sequence.
ACCESSION   DAOQOC010000960 DAOQOC010000000
VERSION     DAOQOC010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78053_length_1411_cov_3.072271"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PL106_13490"
     CDS             complement(<1..268)
                     /locus_tag="PL106_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNA34134.1"
                     /translation="MIAPRTEGRKFIGIDFTKDPLPTGHFERCSFARCSFTRADIAAR
                     TFLDCAFETCELSMARITRTSIRECAFRHCKLLGVSFDHGQTNLF"
     gene            437..>1411
                     /locus_tag="PL106_13495"
     CDS             437..>1411
                     /locus_tag="PL106_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34135.1"
                     /translation="MWWRWLTQRDTWVFIAIACCLRLPLFGLLLREHGLHSAWYGWGA
                     ETGDTPGYFGPMDAYLAGEGYRPDFRMPGYGLPYLLFRLFTTPQGAGSLIIVLQAVLG
                     LASVVVLARCARLLGAPRWAQYACCAVLCLSGRVVIHDVYWFTESLCTSSVIIGTHGW
                     LAYRRSGVRSALLWSGVWLAWAVFLRPVQVVWLLFLAVSILWIGRPGARVRAVAALLL
                     LAPFLLADLWWVRRNAIMHGRFEPLSRGVVMPELASSPMYPLMRFLQATGGNFYHWDP
                     GADIRWFNMREGPYGQQGPRLDRDVVMPGFALCERITEDSLHAWAAEMS"
BASE COUNT          202 a          420 c          497 g          292 t
ORIGIN      
        1 agaacaggtt ggtctgtccg tgatcaaagg aaacgccgag cagcttgcaa tgcctgaacg
       61 cgcattcgcg aatgctcgtc cgggtgatcc gggccatgct caattcacag gtctcgaagg
      121 cgcagtccaa aaaggtccgt gccgcgatgt cagcacgcgt gaagctgcaa cgtgcgaagc
      181 tgcagcgctc gaaatggccg gtcggcaatg gatccttggt gaagtcgatg ccgatgaact
      241 tgcggccttc ggtgcggggg gcgatcatat ccgcggacga acctacacca acgcacttga
      301 cgaaatgggc cgaatggagg atgcgcaggt gctccggtcg cggcgggtgc caggatcccc
      361 ggaccgcgta cccgcttcgg ggtcgggtgg ccgacacccc gtgtgtggtc ccgggttccg
      421 atcgatcttc gcgcgcatgt ggtggcgatg gctcacgcaa cgcgacacgt gggtgtttat
      481 cgcgatcgcc tgctgcctgc ggctcccgct cttcggcctt ctgctgcgcg aacatggcct
      541 gcacagcgcg tggtacggat ggggtgcgga gaccggcgat acgcccgggt acttcggacc
      601 catggacgcg tacctggcag gagagggata ccggcccgac ttccggatgc ccggttatgg
      661 gctgccgtat ctgctcttcc gcctcttcac gacaccccag ggggccggct cgttgatcat
      721 cgttctgcag gccgtgctgg gccttgcgag cgtagtggtg ttggcacggt gcgcccggtt
      781 gctcggtgca ccccggtggg cgcagtacgc ctgctgtgcg gtgctgtgcc tgtccggccg
      841 ggtggtgatc cacgatgtat actggttcac ggagagttta tgtacaagct cggtcatcat
      901 cggcacacac ggctggctgg cgtaccggcg gagcggcgtg cgatccgcac tcttgtggtc
      961 gggtgtgtgg ctcgcctggg ccgtgttcct ccggcctgtt caggtcgtct ggctgttgtt
     1021 ccttgcggtg tccatcctgt ggatcggccg gcccggtgcg cgagtgaggg ctgtggcggc
     1081 cctgctgctg ttggccccct tcctgctcgc cgatctctgg tgggtccgtc gcaacgcgat
     1141 catgcatggc cggttcgagc ctttgagccg gggggtggtg atgcccgagc tcgcgtcctc
     1201 gccgatgtat ccgctcatgc ggttcctgca ggcgaccggt gggaatttct accactggga
     1261 ccccggtgcg gacatccgct ggttcaacat gcgcgaaggt ccgtacgggc agcaaggacc
     1321 ccggctggat cgggatgtgg tgatgccggg gttcgcgctt tgcgagcgca tcaccgagga
     1381 cagtttgcac gcatgggcgg cggagatgtc g
//
LOCUS       DAOQOC010000961         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78274_length_1409_cov_3.766617, whole genome shotgun sequence.
ACCESSION   DAOQOC010000961 DAOQOC010000000
VERSION     DAOQOC010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78274_length_1409_cov_3.766617"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PL106_13500"
     CDS             complement(<1..1382)
                     /locus_tag="PL106_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34136.1"
                     /translation="MYSHHANAFRVEENDLFPATAPLAEAEGVVVGYTQAYNDQVYKN
                     TSHGLYYGFVAEDHCVDMNDPTGTGLAFLCNVNLQNQEEDFRVRSTFPDSPPNHSIKM
                     FQGTTSVSAGNEFTPQQNGSSPYYNYHNAAQVLPITYFWEVPPGDLNTGAYNAPWVQR
                     NANVSTVAHGCPTRIICGGTILQVKSFLDPLIDQERLAYLNLKYVFESLLDGGDFEEL
                     KQTIMESWPQDAWELRNALMERSPYLSGQILKEAGLRNILPHAMYLEICLANPEATQR
                     NGFIRWVQYEMPNPLPEYMVAQIVASWDQRTWRTSLESALGWHKGEYQRLNDQVIGAM
                     LNDTIPQPADSILVRWQMNPSLRARYGEVSTLLELERYTEAEALLNGLADNYRMHATD
                     IQERDDMLALIATVRNAQAADRSIMELDGAEVSILESIAARQPSIGATYARNILCFGY
                     GICTSPITGET"
BASE COUNT          261 a          414 c          417 g          317 t
ORIGIN      
        1 gtctcacccg tgatgggtga tgtgcagatg ccgtaaccga aacataggat gttgcgggca
       61 taggtcgcac cgatggaagg ttgtcgggca gcgatggact ccaagatgga cacttctgct
      121 ccatccagtt ccatgatgct gcggtcagcg gcctgtgcgt tccgcaccgt cgcgatcagg
      181 gccagcatgt cgtccctctc ctgaatatcc gtcgcgtgca tccggtaatt gtccgccaac
      241 ccgttcaaca gagcctccgc ttcggtgtag cgttccagtt ccaacagggt gctcacttcg
      301 ccataacgcg cccgcaagct ggggttcatt tgccagcgta ccaggatgct gtcggccggt
      361 tggggtatgg tgtcattcag catcgccccg atcacctggt cgttcaggcg ttggtactcg
      421 cccttgtgcc aacccagggc cgattctaag ctggtgcgcc aggtcctctg gtcccaggag
      481 gccacgatct gggccaccat gtactccgga agcgggttgg gcatctcata ctgcacccac
      541 cggatgaaac cgttgcgctg ggttgcttcc gggttggcca gacaaatctc cagatacatg
      601 gcgtggggca ggatgttcct caacccggcc tccttcagga tctgcccaga cagatagggg
      661 ctgcgctcca tcagggcatt gcgcaattcc catgcgtcct gcggccagct ctccatgatg
      721 gtctgcttca actcctcgaa atcgccacca tccagcaggc tctcgaacac gtacttcagg
      781 ttcaggtagg ccaggcgctc ctggtcgatc aaaggatcga ggaatgactt cacctgtagg
      841 atggtgccac cacagatgat ccgggtgggg cagccatggg caacggtcga gacgttcgcg
      901 ttgcgctgca cccaaggcgc attgtacgcg ccggtgttca gatcacccgg tggcacctcc
      961 cagaaatagg tgatgggcag tacctgagcc gcattgtggt aattgtagta gggcgaactg
     1021 ccgttctgtt gtggggtgaa ctcgttgcct gctgacacac ttgtggtgcc ctggaacatc
     1081 ttgatgctgt ggttcggtgg agaatcgggg aacgttgacc ttaccctgaa gtcctcctct
     1141 tggttctgga ggttcacatt gcagaggaaa gccaatccgg ttcccgtggg gtcgttcatg
     1201 tccacgcaat ggtcctccgc cacgaagccg taatacaggc catgcgaggt gttcttgtac
     1261 acttggtcat tgtaggcttg cgtatacccc accaccacgc cttcggcctc agcaagcggt
     1321 gcagtggcgg gaaatagatc gttctcctcc acgcggaagg cgttcgcatg gtggctgtac
     1381 acagcgcggt gtcgcccatc gaactcggc
//
LOCUS       DAOQOC010000962         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78288_length_1409_cov_3.324963, whole genome shotgun sequence.
ACCESSION   DAOQOC010000962 DAOQOC010000000
VERSION     DAOQOC010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78288_length_1409_cov_3.324963"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PL106_13505"
     CDS             complement(<1..523)
                     /locus_tag="PL106_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34137.1"
                     /translation="MRVLPDSVPAIAGFTKAIGTVPDVNGLSGEFAPGAPPVRASPTA
                     PKVGGWTLWVVVLIASVLLAVGVLLLARPFRHLVERTGALLDAVLDRRTDDGVRMRNL
                     TGAVWNETTALLGLVVAALIVLVIAAVPLMLLGGAASPGAFLGAPFKGWNILAMLAGT
                     TLPFVVVSRRDRGS"
     gene            complement(451..>1409)
                     /locus_tag="PL106_13510"
     CDS             complement(451..>1409)
                     /locus_tag="PL106_13510"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNA34138.1"
                     /translation="LFADGSYAAKLDMTPNLFRMDREGRIKWVNHELVFHHAMHADAD
                     GFLWTCASDLPLGPGGPMVGRTLYSPSTGRLAFQEDHIVKVDPADGRIVFKRGVAGIL
                     IDNGQQGRVFGGGLVDPIHLNDVVPLMSDGPYWHRNDLLLSVRNRSMILLYRPSTDSL
                     LRVIDGPFFAQHDVNVLSPTRISLFSNRQVFEDQYAATNLLWVPRERGLPIDSITWSQ
                     VFEYDLDSGRFHTVEDEALHRMRARTGTQGMHEILPDGSLYLELPGMRGYVVIREGRV
                     VLNKVFGAPMRVGCTNAPGCACSRTAYRRSPVSPRPSEPCRM"
BASE COUNT          278 a          470 c          449 g          212 t
ORIGIN      
        1 ccgaaccccg gtcccggcgc gacaccacaa cgaacgggag cgtggtgccg gcgagcatgg
       61 ccaggatgtt ccatcccttg aacggagcgc ccaggaacgc gccgggtgaa gcggcaccac
      121 ccagcagcat caacggcacg gcggcgatca caaggacgat gagcgcggca acgacgaggc
      181 cgagcagtgc cgttgtctcg ttccacaccg cgccggtgag gttccgcatc ctgacgccat
      241 cgtcggtgcg gcggtcgagc acggcatcga gcaacgcacc cgtccgttcc acgaggtggc
      301 ggaagggacg ggcgagcagc agcacgccca cggccagcag gaccgaggcg atcagtacca
      361 cgacccataa cgtccaaccg cccaccttcg gagcggtggg gctggcccgc acgggagggg
      421 cacctggagc gaactcgccg gaaagtccat tcacatccgg cacggttccg atggccttgg
      481 tgaaaccggc gatcgccggt acgctgtccg ggagcacgcg caaccaggtg catttgtgca
      541 cccgaccctc atcggcgcac cgaagacctt gttcaggacc acccggccct cgcggatcac
      601 cacgtatccg cgcatgcccg gcagctccag gtacagggag ccatcgggca ggatctcatg
      661 catgccctgt gttcccgtgc gtgccctcat cctgtgcagc gcttcgtcct caacggtatg
      721 gaagcggccg ctatcgaggt cgtactcgaa cacctgcgac caggtgatgc tgtcgatcgg
      781 cagtccccgc tcccgcggca cccagagcag gttggtggcc gcgtactggt cctcgaagac
      841 ctgccggttg ctgaaaaggg aaatgcgcgt gggcgaaagc acgttcacat catgctgggc
      901 gaagaacggc ccatcgatca cgcggagcag gctgtcggtg gacggtcgat agagcaggat
      961 catcgagcgg ttgcgcacac tgagcaacag gtcattccgg tgccagtacg ggccatcgct
     1021 catcagcggc accacatcgt tcagatggat cgggtccaca aggccgcccc cgaacacgcg
     1081 tccctgttgg ccattgtcga tcaggatccc cgccacgccc cgtttgaaca cgatgcggcc
     1141 atcggcggga tccaccttca cgatatggtc ctcctggaag gcgagcctgc cggtggacgg
     1201 cgagtacagc gtacggccga ccattggacc gccgggcccc aaggggaggt ccgaagcgca
     1261 cgtccacagg aagccatcgg catccgcgtg catcgcgtgg tggaagacca actcgtggtt
     1321 cacccacttg atgcgcccct cccgatccat acggaagagg ttgggcgtca tgtccaattt
     1381 cgccgcatag gagccatcag cgaagaggt
//
LOCUS       DAOQOC010000963         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78294_length_1409_cov_3.094535, whole genome shotgun sequence.
ACCESSION   DAOQOC010000963 DAOQOC010000000
VERSION     DAOQOC010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78294_length_1409_cov_3.094535"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..453)
                     /locus_tag="PL106_13515"
     CDS             complement(73..453)
                     /locus_tag="PL106_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34139.1"
                     /translation="MRGTLNISHSASAETDDNETHSLTHAATFAVNYVSESDHNVTTF
                     ADTFPLDAVNNGSIAAILLVNLGDDNALVSFQNSGFLHMVIPPGRFVEVHRFSTNMKA
                     AFSGFAIAAENTSTTVRAIVAWTA"
     gene            complement(466..882)
                     /locus_tag="PL106_13520"
     CDS             complement(466..882)
                     /locus_tag="PL106_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34140.1"
                     /translation="MGSITHSVTWAWSVNGARTSRTFTYTSDTVTRVTRIDLNIPEDD
                     TFNLGSMPNTLIIACNGAANILVTDKANDAPVFAGTNPATTVIHSDATNNVGIDAALD
                     PPNVDIEGLAVIMQTVHASGPNTGGVTILAVWEAAS"
     gene            complement(884..1330)
                     /locus_tag="PL106_13525"
     CDS             complement(884..1330)
                     /locus_tag="PL106_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34141.1"
                     /translation="MTARDIATDAFNDLLSAISEAYSDRGKLASGKTLRGTRVLVRSS
                     SDLSATAELLAPHQWKYVGNGRGPGRRPPIAPIKAWVEARGLNVNAWAVANNIAKFGS
                     FDFRRKRTNIYLDEVKAFEEQKLPGVKERHADLLREKVRNIVNNRN"
BASE COUNT          234 a          401 c          453 g          321 t
ORIGIN      
        1 ggtgccgggc gcggattgaa acgcccgtag gccgaagaaa ggggcgtcgg ggattgcgtt
       61 atatgcagca tcctatgcgg tccatgcgac gatcgcgcgc accgtggtcg aagtgttctc
      121 cgcagcgatg gcgaaacccg agaaggccgc cttcatgttc gtgctgaaac gatgcacctc
      181 cacgaacctg ccgggcggga tcaccatgtg gaggaagccg ctgttctgga atgataccag
      241 cgcgttgtca tccccgaggt tcaccagcag gatcgcggcg atgctcccgt tattcacggc
      301 atcgagcggg aaggtatccg cgaaggtcgt tacgttgtga tcactttcgc taacgtagtt
      361 gaccgcgaag gtcgcggcgt gcgtcagcga atgggtttcg ttgtcatccg tctcagccga
      421 tgcgctgtgc gaaatgttca gtgtgcctct catggtgtgt acttgttagc tggcggcctc
      481 ccatacggcg aggatcgtca ccccgccagt gttggggccg gatgcgtgta cggtttgcat
      541 gataacggcg aggccctcga tatccacgtt cgggggatcc agcgccgcat cgatgcccac
      601 gttgttcgtg gcgtcggaat ggatcaccgt cgttgcgggg ttggtgcccg cgaacacggg
      661 cgcgtcgttc gccttgtccg tcaccaggat gtttgccgcg ccattgcagg cgatgatgag
      721 cgtgttcggc atcgatccaa ggttgaaggt gtcatcctcg gggatgttga ggtcgatgcg
      781 tgtgacccgc gtaacggtgt ccgaggtgta ggtgaaggtg cgcgaggtgc gcgcgccatt
      841 cacggaccac gcccacgtca ctgagtgagt aatggatccc atgtcagttt ctgttgttca
      901 cgatgttacg taccttttca cggagtagat ccgcgtgccg ctccttcacg ccgggtagct
      961 tctgctcctc gaacgccttc acctcgtcaa ggtagatgtt cgtgcgcttg cggcggaagt
     1021 cgaaggatcc gaacttggcg atgttgttcg ccacggccca cgcgttgacg ttcaggccgc
     1081 gtgcctccac ccacgctttg atcggtgcga tcggtggacg ccttccgggt cctcgtccgt
     1141 ttccaacgta cttccattga tgcggtgcca ggagttcggc cgttgcggaa aggtcgctcg
     1201 atgaacgcac aaggacgcga gtgccgcgca gggtcttacc gctggccagc ttgccccggt
     1261 cgctgtatgc ctcgctgatc gctgatagca ggtcgttgaa ggcgtcggtg gcgatgtcgc
     1321 gtgcggtcat agcccccact tattgcgaag atagccctgc acccgcttcc gctccagtgc
     1381 cgagtgcgcg gcattgtata tgatcatct
//
LOCUS       DAOQOC010000964         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78431_length_1408_cov_2.856615, whole genome shotgun sequence.
ACCESSION   DAOQOC010000964 DAOQOC010000000
VERSION     DAOQOC010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78431_length_1408_cov_2.856615"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..306
                     /locus_tag="PL106_13530"
     CDS             148..306
                     /locus_tag="PL106_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HNA34142.1"
                     /translation="MKEQGYIDGDFYFTPEGYVVFTEQYLLRRGYCCGNQCRHCPYGH
                     EAVKRVHG"
     gene            375..>1408
                     /locus_tag="PL106_13535"
     CDS             375..>1408
                     /locus_tag="PL106_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA34143.1"
                     /translation="MSAKNIIKDLVRESQAAIGTLPLIARDVRLPKAEFKVLTVTGIR
                     RCGKSSLLRMDMAAELAAGTARERMFFINLEDERLDAETATLTAIPEAYQELYPGIAL
                     DQVRMYLDEIQIMPRWEQFVVRMSEMEKAVVRISGSNSKLLSREIATTLRGRTLNTPL
                     LPFSLAERLRLRGLVLDPYLPRERARLKAEVRDHLLWGGFPEVITLPRPDKRPQLDEY
                     FNVLLFRDLIERHGILQPVVVKHFLKRMLASATKPVSVSKVDQMLRSAGIRTTKALLY
                     DWLDHALDTHLLIRCDRFGASATERLTSTTRFFCTDNGLLTAVTGSFKDEWGKLLENA
                     TAVEAVRQGWG"
BASE COUNT          270 a          436 c          435 g          267 t
ORIGIN      
        1 gaaactaccg gggttcccgg gggttgttca cgatccattc acatcgcttc gcaccggtcg
       61 ttgtgaataa caacggcgga tcggcccatt tttgtgcccg tggaaaggcc tccgctaccc
      121 cacccggacg acccgcgcgg tcgccgcctg aaggaacagg gctacatcga cggcgacttc
      181 tatttcacgc cggaagggta tgtggtcttc accgagcaat acctgttgcg tcggggctac
      241 tgctgcggta accagtgcag gcactgtccg tacgggcatg aggcggtgaa gagggtacat
      301 gggtaaattc gtgtctgcta tcgatctgat atttgcaaat tcgtggttgt tatcaataca
      361 tttggcaggc cgatatgagc gcgaagaaca tcatcaagga tctggtccgg gagagtcagg
      421 cggcgatcgg gacccttccg ctcatcgccc gtgacgttcg gctcccgaag gcggaattca
      481 aggtcctcac ggtgacgggg atccggcgtt gcggcaagag cagccttctg cgcatggaca
      541 tggccgcgga attggcggcc ggcacggcgc gtgaacggat gttcttcatc aacctggagg
      601 atgaacggct cgatgccgag acagcaacac tcacggccat ccccgaagcc tatcaggaac
      661 tgtatcccgg catcgcgctc gaccaggtgc gcatgtacct cgatgagata cagatcatgc
      721 cccgctggga gcagttcgtg gtgcgcatga gcgagatgga gaaggccgtg gtgcgcatat
      781 caggcagcaa ctccaagctc ttgagccgcg agatcgccac gacgctccgc ggccgcacgc
      841 tcaatacgcc gctgctcccc ttctcgctgg ccgagcggct ccggttgcgt ggcctcgtgc
      901 tggacccgta cctcccccgg gaacgtgcgc ggctgaaggc ggaggtgcgc gaccatttgt
      961 tgtggggcgg ctttcccgaa gtgatcacgt tgccccggcc ggacaagcgg ccgcaattgg
     1021 atgagtattt caacgtgctg ctcttccgcg acctgatcga acgccatggc atcctgcagc
     1081 cggtggtggt caagcacttc ctgaaacgca tgctcgccag cgccaccaag ccggtgtcgg
     1141 tgagcaaggt ggaccagatg ctccgttcag cgggtatacg caccaccaag gccctgctct
     1201 acgactggct cgaccatgcg ctcgacacgc acctgctgat ccgctgcgat cgcttcggcg
     1261 cctcggccac cgaacgcctc acgagcacca cacgcttctt ctgcacggac aacggcctgc
     1321 tcaccgccgt caccggttcc ttcaaggacg aatggggtaa actgctggag aacgccacgg
     1381 ccgtggaagc cgtacgccag ggctgggg
//
LOCUS       DAOQOC010000965         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78899_length_1403_cov_4.691395, whole genome shotgun sequence.
ACCESSION   DAOQOC010000965 DAOQOC010000000
VERSION     DAOQOC010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78899_length_1403_cov_4.691395"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PL106_13540"
     CDS             <1..1327
                     /locus_tag="PL106_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA34144.1"
                     /translation="YPRSIGYLYQATAIDPTCAKCFLERGISQLSMDSVDAARVSLQE
                     AQRLKLSVEQGRRASNMAVDWFKRAESEAHEKGNPVLAVRYYAGATVLSPDSLRWLAR
                     RALNLMEIPRWEEALGIWDEYITRVDGDYWGRINRARCLLNMERYAEVDAEYDKVERN
                     DLKGVYTKENNLVGGEACFRKGDLTCAEARLMKALRKDGNDYRVLSMLSECVYARKDY
                     DQAEKYAKDAVSNFEKQAKATGADQGDPRVWYRLGLIQQANGKFKESLQSFDKAKYWG
                     QDRDEIFKGKGRSYQLLGDLPQAVDQYSELLRANEDPQSRILRAECYVKLGQYIEAER
                     DLLAVQEKHPEVAARPEFLAQLAFAHVLTGAKREAGEAITSGKLADPDYVMLLLADVA
                     YKWKFDRHEEAVTQLSDLVRKGAADEKKLKELPVLKDLVSSPMWKNRGK"
BASE COUNT          319 a          378 c          467 g          239 t
ORIGIN      
        1 ttatccaagg tcgatcgggt acctgtacca agcgaccgcc atcgacccca cctgtgcgaa
       61 atgtttcctg gaaagaggga taagccagct gagcatggac agtgtggacg cggcccgggt
      121 atcgctacag gaggcccagc gcctcaagct gtccgtggaa cagggcaggc gcgccagcaa
      181 catggccgtc gactggttca aacgggccga gagcgaagcg cacgagaaag gcaatccggt
      241 gctcgccgtg cgctactatg ccggagccac ggtgctgagt cccgattcgc tccgctggct
      301 ggcgcgcaga gccttgaatc tgatggagat cccgcggtgg gaggaggcac tcggcatctg
      361 ggatgaatac atcacccggg tcgatgggga ctattggggc cgtatcaacc gcgcccggtg
      421 cctgctgaat atggaacggt acgcggaggt ggacgcggag tacgacaagg tggaacgcaa
      481 cgatctgaag ggggtgtaca ccaaggagaa caatctggtg ggcggagaag catgcttccg
      541 taaaggcgac ctcacgtgtg cggaggcccg gctcatgaaa gccctgcgca aggacgggaa
      601 cgactaccgt gtgttgtcca tgctttccga gtgcgtgtac gcgcggaagg attacgacca
      661 ggccgagaag tatgcgaagg atgccgtgtc caacttcgag aaacaggcga aggccaccgg
      721 tgccgatcag ggcgatccgc gggtctggta ccgcttgggc ctgatccaac aggccaacgg
      781 gaagttcaag gaaagcctgc agagcttcga caaggcgaaa tactgggggc aggatcggga
      841 tgagatcttc aaggggaaag gccgttccta ccaattattg ggcgatctgc cccaagctgt
      901 tgatcagtac agcgaacttc ttcgtgccaa cgaggatccc cagtcccgga tcctcagggc
      961 ggaatgctac gtcaagctcg gccagtacat cgaagcggag cgggatctgc tcgctgtaca
     1021 ggagaagcat cctgaggtgg ccgcacgacc ggagttcctt gcgcaattgg cgttcgcgca
     1081 cgtgctcacg ggtgccaagc gcgaagcggg ggaggcgatc acgagcggca agttggccga
     1141 ccccgactat gtgatgctcc tgctggccga tgtggcctac aaatggaagt tcgaccggca
     1201 cgaggaggcg gtcacacaac tgtccgacct ggtgcgcaaa ggcgccgctg atgagaagaa
     1261 gctgaaagaa ctacccgtat tgaaggacct cgtatcatcc cccatgtgga agaaccgggg
     1321 caagtagtca tcggtagcca catcgaggcc atgtgcaggc ggcgatgatg tcgttcatgg
     1381 atcgtatcac gtgcgcttga acg
//
LOCUS       DAOQOC010000966         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78904_length_1403_cov_4.251484, whole genome shotgun sequence.
ACCESSION   DAOQOC010000966 DAOQOC010000000
VERSION     DAOQOC010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78904_length_1403_cov_4.251484"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PL106_13545"
     CDS             complement(<1..281)
                     /locus_tag="PL106_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglyceryl transferase"
                     /protein_id="HNA34145.1"
                     /translation="MANEGVGWMDRLAKRWGVSPSRVTIILVVFACTGFTVVYLKRPL
                     IDLVAGDGEKPLWFSILYYVLILPVYNVLLLVYGAILGQFRFFWEFEKR"
     gene            complement(323..1270)
                     /locus_tag="PL106_13550"
     CDS             complement(323..1270)
                     /locus_tag="PL106_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNA34146.1"
                     /translation="MSAKHTPRNLLLLIGGLLLLWWLGGLISPAAKQRSFRSSLLDLD
                     TNLIRSFIITPAPVKQLPPFLFQRIGGTWRIHWVNDSADADPAPIHDMLRSFSHMRVV
                     RYVGQVDDIGGHYALNDSLADHVRIQLAEREVDLLVGRVTDTQPPLTIVRLPGDESVY
                     AIDGPLGRYADQTFGDWLPKYLVTGDPANWARLTFNFPTHVGYVMERRNGRWTIDGTE
                     LDPERTERYLGALANAKGLSAVDPSDTLDAVPMYRLMVDDTTRDQPVIVVVFSSKGRF
                     IVRSTLNPTTVMPFDEQNEVMRMFRPPGAFMPRQEADVP"
BASE COUNT          282 a          438 c          431 g          252 t
ORIGIN      
        1 aaacgcttct cgaactccca gaagaaccgg aactggccga ggatcgcgcc gtacacgagc
       61 agcagcacgt tgtacacggg gaggatcagc acgtaataga ggatcgagaa ccacaggggc
      121 ttctcaccat cgcccgccac caggtcgatc agggggcgct tgaggtacac gaccgtgaaa
      181 cccgtgcatg cgaagaccac caggatgatc gtcactcgcg aaggacttac accccagcgc
      241 ttcgccaagc ggtccatcca acccacccct tcgttcgcca tggcgcgaat atcggagcca
      301 cggcttgttg taccgaactc cgttatggca catcggcctc ctgccttggc atgaacgcac
      361 caggtggcct gaacatccgc atcacctcgt tctgctcatc gaacggcatc accgtggtcg
      421 ggttcaaggt gctgcggacg atgaagcggc ccttgctgga gaagaccacc acgatcaccg
      481 gctggtcgcg tgtggtgtcg tccaccatca gcctgtacat gggcacggca tccagcgtgt
      541 ccgaaggatc caccgcactg agtcccttgg cgttggcgag cgctccgagg tacctctccg
      601 ttcgctccgg gtcgagttcg gtcccgtcga tggtccagcg gccgttcctt cgctccatca
      661 cgtatccgac atgggtgggg aagttgaacg tgagccgggc ccagttggca ggatctcccg
      721 ttaccagata cttgggcagc caatccccga aggtctgatc ggcgtacctt cccaatggac
      781 catcgatggc gtacacgctc tcatccccag gcagacgaac gatggtcaag ggcggttgcg
      841 tatcggtcac ccgaccgacg agcaggtcca cctcccgttc ggccaactgg atgcgcacgt
      901 ggtcggccag gctgtcgttg agcgcgtaat gcccaccgat atcgtccacc tgccctacgt
      961 accgcaccac gcgcatatgg ctgaaggagc gaagcatgtc gtggatcggt gccggatccg
     1021 catcggccga atcgttcacc cagtggatgc gccaggtgcc gccgatgcgc tggaacagga
     1081 acgggggcaa ttgtttgacc ggtgcggggg tgatgatgaa tgatcggatc aggttcgtgt
     1141 ccagatcaag aagtgaactg cggaatgagc gctgtttcgc cgcaggactg atcagcccac
     1201 ccagccacca cagcaggagc aacccgccga tcagcagcag gaggttccgg ggcgtgtgct
     1261 tagcggacat gttcaagggc catgcgtctt gtgcgctgtg cgcgacgcca ggcgcggcgt
     1321 gcgatgcctg cggataccac aagcagcacg ggcagcagga ggtccagcca cttcaacagg
     1381 gtgcgcatgg cgtcgctgag ttg
//
LOCUS       DAOQOC010000967         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78934_length_1403_cov_3.019288, whole genome shotgun sequence.
ACCESSION   DAOQOC010000967 DAOQOC010000000
VERSION     DAOQOC010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78934_length_1403_cov_3.019288"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PL106_13555"
     CDS             complement(<1..1389)
                     /locus_tag="PL106_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNA34147.1"
                     /translation="MTGATAGHTFTSTASIAGTYITVRAGVYNGAVVAAGTTPLNWTA
                     AAGGSYFIHYNTNAACGTASTCMTTTVQNTSPIPGCTNTSAYPTAFVAPAPGVTYTIT
                     TCQYQQEYNQMTGATAGHTFTSTASIAGTYITVRAGVYNGTVVASGTTPLNWTATAGG
                     TYFLHYNTNSSCGTATTCMTTTVQNTTTNDLCANAIGVGTLPYTSPLVSNAAATDDAP
                     TTTCDGPYKNMWWAVTGVCGTMTAYTCGSSFDTEIAVFTGSCGSFTQVACNDDATAGP
                     CASTLQSYVTWNSTAGTTYYITAGSYGALSSTGNIQLNVTGGDITPPSITCPATQSAC
                     GATMPDYTSLASASDNCTANGSISITQSPTPGSALGLGNTTVTLTATDAANNQGQCTF
                     TVSQPDSDGDNTADCVDGCPSDPAKTAPGVCGCGVADVDADGDGVYTCQGDLCDNDPL
                     KTTPGICGCGVAD"
BASE COUNT          237 a          398 c          445 g          323 t
ORIGIN      
        1 atcggcaaca ccgcaaccac aaatgcccgg tgtggttttc aaaggatcgt tatcgcagag
       61 gtcgccctgg caggtgtata cgccgtcacc atcggcatcc acatcagcaa cgccgcaccc
      121 gcatacaccg ggggcggttt ttgcaggatc gctcggacag ccatccacgc aatccgctgt
      181 attgtccccg tccgagtcgg gctggctaac cgtgaaggtg cattgccctt gattgttggc
      241 ggcatcggtc gcggtgaggg tcacggtcgt gttccccagg cccaaggcac tacccggcgt
      301 tggtgattgc gttatactga tcgatccgtt cgcggtgcaa ttgtcactcg cgcttgccaa
      361 tgaggtgtag tcgggcatgg tagccccaca tgcgctctgt gttgcaggac aggtgatgga
      421 cgggggcgtg atatcgccac cggtgacatt caattggatg ttgccggtgg agctgagtgc
      481 accataactg cccgccgtga tgtaataggt cgttcctgcg gtactgttcc aggtcacata
      541 actctgtagc gtacttgcgc aggggccggc agtggcgtca tcgttgcagg ccacctgtgt
      601 gaatgagcca caggaccctg tgaataccgc gatctccgtg tcgaacgaac tcccgcaggt
      661 gtacgcggtc atcgtgccgc ataccccggt caccgcccac cacatgttct tgtacgggcc
      721 gtcgcacgtg gtcgtcggtg cgtcatccgt tgctgcggcg ttcgatacca aggggcttgt
      781 atagggcagc gtgcccactc caatagcgtt cgcgcagagg tcgttggtcg tggtgttctg
      841 gaccgtggtg gtcatgcacg tggttgccgt tccacatgag gagttcgtgt tgtagtggag
      901 gaaatacgtg cctcctgcgg tggccgtcca gttcaacggt gttgtgcctg acgccaccac
      961 cgtgccgttg tatacacccg cccgcaccgt gatgtacgtg cccgcgatgc tcgccgtgga
     1021 ggtgaaggtg tggcccgctg ttgcacccgt catctggttg tactcctgct ggtattggca
     1081 cgtggtgatg gtgtacgtca caccgggagc aggcgcgaca aaggcggtcg gataggcaga
     1141 agtattggtg catccgggaa tcggagatgt gttctgcacc gtggtcgtca tacaagtgga
     1201 cgctgttccg caggccgcat tcgtgttgta atggatgaag tagctgcccc ctgcggcggc
     1261 cgtccagttc aatggtgttg tgcctgctgc caccacggca ccgttgtaca cacccgcacg
     1321 cacggtgatg tatgtacccg cgatgctggc cgtggaggtg aaggtgtggc ccgctgttgc
     1381 acccgtcatc tggttgtact cct
//
LOCUS       DAOQOC010000968         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_78971_length_1403_cov_2.227003, whole genome shotgun sequence.
ACCESSION   DAOQOC010000968 DAOQOC010000000
VERSION     DAOQOC010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78971_length_1403_cov_2.227003"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PL106_13560"
     CDS             <1..378
                     /locus_tag="PL106_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34148.1"
                     /translation="GWKVAWVHGADIHQMEPRMGDVTEGYGSARGRARLALPDLYDRL
                     GWATFQGIDGPVETRADVQRWYNTHVADFANLRTVTGNRLNRSAIRRMAKADRLKRKA
                     ELREALRNRISPLTGKPVLVPKR"
     gene            complement(430..756)
                     /locus_tag="PL106_13565"
     CDS             complement(430..756)
                     /locus_tag="PL106_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34149.1"
                     /translation="MFVTFPGGLTANVPQQTVQTGAFGTAAYPPTLENGSLMRPISSG
                     SANPYRTKTFGELVVFLDNGNVAGTATFDSQAAATTALENIGIALEAEDAAIILYADG
                     TYATAS"
     gene            complement(845..>1403)
                     /locus_tag="PL106_13570"
     CDS             complement(845..>1403)
                     /locus_tag="PL106_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34150.1"
                     /translation="GTTNAIEVIEVPALVKGTQGARGFSVQPLRDAGRTSVVYRPSHG
                     IGAYAGFTSDTAYAATMYKATGDGTVSASGTSFVIPSGKTFRVQAFRATMMVQSTSAV
                     VTRVQVGLLINPAGAVTTASSLALTMRLTGQATAGLTDHEDITIPEGFDIVGDGTLQI
                     GVYLLANFASGSGPTIQFNLVGYEF"
BASE COUNT          279 a          418 c          442 g          264 t
ORIGIN      
        1 gggtggaagg tggcgtgggt ccatggggcg gatatccacc agatggaacc ccgcatgggc
       61 gatgtcaccg agggctatgg cagcgcgcgg gggagggccc gccttgcgtt gccggacctg
      121 tacgaccgtt tagggtgggc caccttccaa ggcatcgacg gccccgtgga aacgcgcgcg
      181 gacgtgcagc ggtggtacaa cacccatgtg gcggacttcg ccaacctgcg aacggtcacg
      241 ggcaatagat tgaaccggtc cgccatacgc cgcatggcca aggccgaccg cttgaagcgc
      301 aaagccgagt tgcgcgaagc cctgcggaac cggataagcc cgcttacagg aaagccggta
      361 ttggtaccaa agagataggg cgcccattgg acaccctatc ttcaggagca ctggacaaac
      421 aaaatgcggt caggatgcgg tggcgtaggt gccatccgcg tagaggatga tcgccgcgtc
      481 ctcggcttcg agggcgatgc cgatgttctc caatgcggtc gtggcggcgg cctggctgtc
      541 gaaggtggct gttcccgcca cgttgccgtt gtcgaggaac acgaccagct caccaaaggt
      601 cttggtacgg taggggttgg cggagcccga ggagatcggg cgcatcagcg agccgttctc
      661 cagggtagga gggtaggcgg ccgttccgaa agcgccggtc tggacggtct gttgcgggac
      721 gttggcggtg aggccgccgg ggaaggtcac gaacatggct gcggtgttgt tggtttgcct
      781 gtggggtcaa atgtaatgcg atcggggatg aagtgatata gcagcgctat cacatcattc
      841 gatgtcaaaa ttcgtaaccc acaaggttga actggatcgt cggccccgat cctgacgcaa
      901 agttcgccaa cagataaacc cctatctgca aggtcccatc cccgactata tcaaacccct
      961 cgggtatcgt tatatcctca tggtccgtca agcccgccgt ggcctgcccc gtcaaccgca
     1021 tggtcagagc gagcgacgag gccgtcgtga ccgccccagc cgggtttatc agaagcccca
     1081 cctgtacccg agtaacaacc gctgacgtac tctgcaccat catcgtggct cggaacgcct
     1141 gcactcggaa tgtcttgccg cttgggatca cgaacgatgt tccagaagct gacaccgtac
     1201 cgtcaccagt ggccttatac atcgtggccg cgtatgctgt gtccgatgtg aagccagcat
     1261 aagcgccgat gccgtggctg ggcctataca ccacgctcgt cctcccggca tcccttagtg
     1321 gttgcacact gaatccgcgc gccccctgcg ttcctttgac cagggcaggc acctcgatca
     1381 cctcgatggc attcgtggta ccc
//
LOCUS       DAOQOC010000969         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_79093_length_1402_cov_2.376392, whole genome shotgun sequence.
ACCESSION   DAOQOC010000969 DAOQOC010000000
VERSION     DAOQOC010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79093_length_1402_cov_2.376392"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PL106_13575"
     CDS             complement(<1..211)
                     /locus_tag="PL106_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNA34151.1"
                     /translation="MSWFNSFFTQEIAIDLGTANTLIIHNDKVVVDEPSIVAVDRTSG
                     RVIAIGRAAQQMHGKTHENIKTVRPL"
     gene            complement(222..>1402)
                     /gene="purH"
                     /locus_tag="PL106_13580"
     CDS             complement(222..>1402)
                     /gene="purH"
                     /locus_tag="PL106_13580"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009415862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNA34152.1"
                     /translation="ESGIIEKIDIGGISLIRAGAKNHADVTIVASKAQYPELFRILRE
                     QNGVTSLVQRKTLAAQAFDVSSRYDGAIHDWFLGDAGDQLLLPVPVHSLRYGENPHQS
                     ARFHGDLAELFEQHNGKELSYNNLLDLDAAIALIDDLAAIKGPGEAFAILKHNSACGA
                     AIRPTLRTAWDAALAGDPVSAFGGVLIATAPIDRATAEAMDSIFFEIVCASGYSPEAL
                     EILSRKKNRIILTRRGVKRPALNYRSALNGVLEQTPDNVVNDAASMRTITEKMPSAQD
                     LDDLVFANVLVKHTKSNAIVLAKDRQLLASGTGQTSRVDALEQAIAKAAKFNFNLEGA
                     VMASDAFFPFPDCVTIAARAGIKAIVQPGGSIKDQDSINAADANGLAMVLTGDRHFKH
                     "
BASE COUNT          243 a          416 c          444 g          299 t
ORIGIN      
        1 gcaggggccg cacggtcttg atgttctcgt gcgttttgcc atgcatctgc tgcgccgcgc
       61 ggccgatcgc gatcacgcgc ccgctggtgc ggtccaccgc cacgatgctg ggctcgtcca
      121 ccaccacctt gtcgttgtgg atgatgagcg tgttggccgt gccgaggtcg atggcgatct
      181 cctgggtgaa gaaactgttg aaccagctca tgcgtgggta ttcaatgttt gaagtggcga
      241 tcgcccgtta gcaccatggc gaggccgttg gcgtccgctg cgttgatgct gtcctggtcc
      301 ttgatgctgc cgcccggctg aacgatggcc ttgatgccgg cacgggcggc gatggtcacg
      361 caatccggga aggggaagaa cgcatcgctc gccatcaccg cgccctcgag gttgaaattg
      421 aacttcgcag ccttggcgat cgcctgttcc aaggcatcca cccggctggt ctgtcccgta
      481 ccactggcca gcaactgccg gtccttggcc aggacgatgg cgttgctttt cgtgtgtttc
      541 accagcacat tggcgaaaac gagatcatcc agatcctgtg ctgaaggcat tttttccgtg
      601 atcgtacgca tgctggcagc gtcgttcacc acgttgtccg gggtctgctc cagcaccccg
      661 ttgagggcgg agcggtagtt cagggcggga cgttttacgc cacggcgggt caaaatgatg
      721 cggttcttct tcctggaaag gatctccaag gcctcgggcg agtacccgga agcacaaacg
      781 atctcgaaaa aaatggagtc catggcttcc gccgtggccc tgtcgatggg tgcggtggcg
      841 atgagcacac cgccgaaggc gctcaccgga tcaccggcca aagccgcgtc ccatgcggtc
      901 ctcagtgtgg gccggatggc cgcgccgcag gcgctgttgt gtttcaggat ggcgaaggcc
      961 tctccaggtc ctttgatggc cgcgagatcg tcgatcaagg cgatcgccgc atccaggtcc
     1021 aagaggttgt tgtagctgag ttccttgccg ttgtgctgct cgaagagctc ggccagatcg
     1081 ccgtggaacc gggccgattg gtgcgggttc tcaccgtagc gcagggaatg tacgggcacc
     1141 ggcaggagca attgatcccc tgcatcgccg aggaaccagt cgtggatggc accatcatag
     1201 cgagaactca catcgaaagc ctgggcggcc agtgtcttgc gttggaccag gctggtgaca
     1261 ccgttctgtt cacgaaggat cctgaacagc tccgggtact gggcctttga cgccacgatg
     1321 gtcacgtccg catggttctt cgccccggcc cggatgagcg aaataccacc gatatcgatc
     1381 ttctcgatga tcccgctctc gc
//
LOCUS       DAOQOC010000970         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_79376_length_1399_cov_6.479167, whole genome shotgun sequence.
ACCESSION   DAOQOC010000970 DAOQOC010000000
VERSION     DAOQOC010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79376_length_1399_cov_6.479167"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PL106_13585"
     CDS             <1..785
                     /locus_tag="PL106_13585"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34153.1"
                     /translation="NGISEWLSALVLSDSSVLVVGDKGVNFGNSSAIISRYTSDGDLL
                     WTDDVVTGIGGPSYINRLTDAFQRPDGTIVVAGQYEWQGTGFFGFVQAYSMEGDSLWR
                     RAFRYMTDSVYSYPAIWDLEPTSDGGMILTGAATIMPGYSEHLWLVKTDSLGCVVPGC
                     QNVGVQEFTIDLQEHLDVSPNPAHDRVNVLLTLPETGEIEGQAQVLLLDATGRTVLEQ
                     PMQRNLNQLRTTVEVSALPAGMFYLHLCDAQRWLAGSKVVVE"
BASE COUNT          303 a          368 c          420 g          308 t
ORIGIN      
        1 agaacgggat atccgaatgg ctctcggccc tggtcttgtc cgactcgagc gtgctggttg
       61 ttggtgataa aggcgtcaac ttcgggaaca gctctgctat catctcgcgc tatacatcgg
      121 atggtgatct cttgtggacc gacgatgttg tcacgggcat cgggggaccg tcctacatca
      181 accggctcac ggatgcgttc cagcgacctg atggtacgat cgtggtggcc gggcagtatg
      241 aatggcaggg caccgggttt ttcggcttcg ttcaggcgta cagcatggaa ggtgattccc
      301 tgtggcggcg ggcgttcaga tacatgacgg atagcgtgta cagctatccg gccatttggg
      361 acctggagcc cacgagcgat ggagggatga tccttactgg cgcggccacc atcatgcccg
      421 gctattcaga gcatttgtgg ctggtaaaga ccgacagcct gggctgcgtg gtaccgggct
      481 gccagaatgt gggcgtacag gagttcacca tcgatctgca ggaacatctg gacgtgagcc
      541 ccaaccccgc ccatgaccgg gtgaacgtgc tgcttacact acccgaaacg ggtgagatcg
      601 aaggccaggc acaggtattg ttgttggatg ccaccggccg aacggtgttg gaacaaccca
      661 tgcaacggaa cttgaaccaa ctacgcacca ctgtggaggt gagcgccttg ccagcaggga
      721 tgttctactt gcacctgtgc gatgcccagc ggtggctggc ggggagcaaa gttgttgtag
      781 agtgaaaaaa atgccattcc acgtcaccct gagcggagcc gaagggtggt ggtggaatag
      841 cgctattgca tagggcccaa gacctgtcac gctgagcttg acgaagcgct accatgatca
      901 tgtgcatgtg tgtatagcgc ttcggcaggc tcagcgtgac agttcaggag gatcctcaag
      961 acagtgtcca agcgatagcg tagcattgct tcaacactcc catgcattgt cagggtgaga
     1021 actcccatgc attgtcacgg tgagaactcc catgttttgt cacgctgagc ctgccgaagc
     1081 gctaccatga tcacgtgcat gtgtgtatag cgcttcggcg cgctcagcgt gacagttcag
     1141 gaagatcctc aaggcagtgt ccaagcgata gcgtagcatt gcttcaacac tcccatgcat
     1201 tgtcagggtg agaactccca tgcattgtca cggtgagaac tcccatgttt tgtcacgctg
     1261 agcctgccga agcgctacca tgatcacgtg catgtgtgta tagcgcttcg gcgcgctcag
     1321 cgtgacagtt caggaagatc ctcaaggcag tgtccaagcg atagcgtagc attgcttcaa
     1381 cactcccatg cattgtcag
//
LOCUS       DAOQOC010000971         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_79429_length_1399_cov_2.810268, whole genome shotgun sequence.
ACCESSION   DAOQOC010000971 DAOQOC010000000
VERSION     DAOQOC010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79429_length_1399_cov_2.810268"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PL106_13590"
     CDS             complement(<1..568)
                     /locus_tag="PL106_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA34154.1"
                     /translation="MSSNDGHVKTSTEGGIATITFHHPKSNSLPGHVLRGMAQAIGDA
                     GKRSDVRVIILRSEGDKAFCAGASFDELVAIDNKEKGLEFFSGFAHVINAIRKAPVLV
                     IGRIHSHCVGGGVGLACAVDIAYAHESASARLSELAVGIGPFVVGPAVERKVGTGTFG
                     LLSATPAMRRSAAWCEQRGMYAEVFTTVE"
     gene            610..>1399
                     /locus_tag="PL106_13595"
     CDS             610..>1399
                     /locus_tag="PL106_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA34155.1"
                     /translation="MPDRRSFIQQSAALLGGLALHTNVEAAFPGLAAGTSPRPATLHD
                     PGDEEAFWQQVRQAYAASPTLINLNNGGVSPSPRATAEALDHYTRMCNEAPSYYMWRI
                     LDQDREPLRMNLAALAGTSPEELAICRNTTEALNTIIFGLPLQKDDEVVVSTCDYPNM
                     ANAWKQRSLRDGVKLVYADPVLPSADEDAIVRAYTDRFTARTKLVHVTHVINWNGQIM
                     PVRRIADEAHKRGIEVLVDGAHAFAVLDHKITDTGCDYWGTSLHK"
BASE COUNT          266 a          487 c          402 g          244 t
ORIGIN      
        1 cttcaaccgt ggtgaatacc tccgcgtaca tcccgcgctg ctcgcaccaa gcggcgctgc
       61 ggcgcatggc gggcgtggca ctgagcaacc cgaaagtccc cgtgcccact ttgcgctcaa
      121 cggcgggccc caccacaaag gggcctatcc ccacggcgag ttcgctcaag cgggcgctcg
      181 cgctttcgtg ggcgtaggcg atgtccaccg cacaggccag gcccacaccc ccgcccacgc
      241 aatggctgtg gatccttccg atcaccaaca ccggcgcttt gcgaatggcg ttgatcacgt
      301 gcgcgaaacc gctgaagaac tccagtccct tctccttgtt gtctatcgcc accaactcat
      361 cgaaactggc accggcacag aacgccttgt ccccctcgct gcgcagaatg atcacccgga
      421 catcggatcg cttgcccgcg tccccgatcg cctgggccat cccgcgcagc acgtggcccg
      481 gtagactgtt gctcttgggg tgatggaagg tgatcgtggc gatgccgcct tcggtgctgg
      541 tcttcacgtg accgtcgttg ctgctcatgc gtgcaaagga atggtgtagc cgcatagctt
      601 cgggccatca tgcccgatcg ccgctccttc atccaacaga gtgccgccct gctgggtggt
      661 ctcgctttgc acacgaatgt ggaagccgcc ttccccggcc tcgccgcagg cacctcacct
      721 cgtccggcca cgctgcacga ccctggcgac gaagaggcct tctggcaaca ggtgcgtcag
      781 gcctatgccg ccagtcccac gctgatcaac ctgaacaatg gcggcgtgag cccttcgcca
      841 cgcgccacag cggaagcact ggatcattac acacgcatgt gcaacgaggc ccccagctac
      901 tacatgtgga ggatcctcga tcaggaccgc gaacccctgc gcatgaacct ggccgcgttg
      961 gcggggacct cgcccgagga actcgccatc tgccgcaaca ccaccgaggc cctcaacacc
     1021 ataattttcg gacttccgtt acagaaggac gacgaggtgg tcgtgagcac ctgtgactat
     1081 cccaacatgg ccaacgcgtg gaagcagcgt tccttgcgcg acggcgtgaa gctcgtgtac
     1141 gccgatcccg tactgccaag cgccgatgag gatgccatcg tgcgagcgta caccgatcgc
     1201 ttcaccgcgc ggaccaagct ggtgcatgtg acccacgtga tcaattggaa cggccagatc
     1261 atgcccgtgc gcaggatcgc ggacgaagcg cacaagcgcg gcatcgaagt gctcgtggac
     1321 ggggcccacg ccttcgccgt gctcgaccat aagatcaccg ataccgggtg cgattactgg
     1381 ggcaccagcc tgcacaagt
//
LOCUS       DAOQOC010000972         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_79449_length_1399_cov_2.414435, whole genome shotgun sequence.
ACCESSION   DAOQOC010000972 DAOQOC010000000
VERSION     DAOQOC010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79449_length_1399_cov_2.414435"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1399
                     /locus_tag="PL106_13600"
     CDS             40..>1399
                     /locus_tag="PL106_13600"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNA34156.1"
                     /translation="MTRAHFIRNALLIVVALVLVNMVGQRFKFRVDLTADQRYSLSPA
                     TLSLLNELPEAVTITAWFTEKMPPDLAVARQDFKDLLVEYANRSNGNVVFEFIDPNSS
                     EPLIKKAQEAGIQPLLATTRTQDRMENLQVYMGCTVQMGERSTVIPAIQQGSALEWAV
                     SSAIKEVSALEKPLVGIVQMHGEASMGELNQLVQNLNVTYSVQATAIYDQVPINDGFA
                     ALVFVGPRDSIPPMQLDRLDDFLAKGKGVVIAYDPVRSDLGSSPVATVVRTNLEPWLE
                     RHGLRVDPFIVVDQKCNQVQVMQQVGSMSRPVAINFPYFPVIDNFADHPVTQGVDVVT
                     FQFCAPITPVDPDSSLRYSPILVTSAQSGTLPSPHVIDIRKQWAPADFLAGPQTVGAE
                     VSGRFGNGAEQGRLILFTNGGFCVSGSSERPVQLPPGNLDVVVNAVDRVSGQNALAGL
                     RGK"
BASE COUNT          287 a          422 c          444 g          246 t
ORIGIN      
        1 gaactgaacc tgggcaaacg cctgatcaac gggtgagcga tgacacgggc acatttcatc
       61 cgcaacgcct tgctcatcgt ggtggcactg gtgctggtga acatggtggg ccagcgcttc
      121 aaattccggg tcgatctcac ggccgatcag cgctactcgc tgagccccgc aacgctcagc
      181 ctgctgaatg agctgccgga ggcggtcacc attacggcct ggttcaccga aaaaatgccg
      241 cccgacctgg ccgtggcccg gcaggacttc aaggacctgc tggtggaata cgcgaaccgc
      301 agcaacggca acgtcgtctt cgagttcatc gaccccaaca gctccgaacc gctgatcaaa
      361 aaagcgcaag aagccggtat tcagccactg ctggccacca cgcgcaccca ggaccgcatg
      421 gagaacctgc aggtgtacat gggctgtacg gtgcaaatgg gcgaacgcag cacggtgatc
      481 cccgccatcc aacagggatc cgccctggaa tgggccgtca gcagcgctat caaggaagtg
      541 agcgcgctgg agaagccact ggtgggcatc gttcagatgc atggcgaagc gagtatgggc
      601 gagctgaacc agctggtgca gaacctgaac gtgacgtaca gcgtgcaagc cacggccatc
      661 tacgatcagg tccccatcaa cgatgggttc gccgcgttgg tgttcgttgg accgcgcgac
      721 agcatacctc ccatgcagtt ggaccggctg gatgatttcc tggccaaggg caagggcgtc
      781 gtgatcgcgt acgatcccgt gcggagcgac ctgggatcct ccccggtggc aacggtcgtt
      841 cgtaccaact tggaaccctg gttggagcgg catggcctgc gggtggatcc gttcatcgtg
      901 gtggaccaga aatgcaacca ggtccaggtg atgcagcagg tgggcagcat gagcagaccg
      961 gtggccatca acttcccgta cttcccggtg atcgacaact tcgcggatca tccggtaacg
     1021 cagggcgttg atgtggtgac cttccaattc tgcgcaccga tcaccccggt cgatcccgac
     1081 agttcgctga ggtactcacc gatcctggtc accagcgcac agagcggcac gctgccctcg
     1141 ccgcatgtga tcgacatccg caagcagtgg gccccggcgg acttcctcgc cggaccacag
     1201 accgttggtg ccgaggtgag cgggcgcttc ggcaacggag cggagcaggg gcgattgatc
     1261 ctgttcacca atggcggctt ctgtgtgagc ggaagttccg aaaggccggt acaattgccg
     1321 cccggcaacc tcgatgtcgt ggtgaatgcc gtggaccggg tgagcggaca gaacgccttg
     1381 gccggtcttc gtggcaagg
//
LOCUS       DAOQOC010000973         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_80012_length_1394_cov_3.471994, whole genome shotgun sequence.
ACCESSION   DAOQOC010000973 DAOQOC010000000
VERSION     DAOQOC010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80012_length_1394_cov_3.471994"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PL106_13605"
     CDS             complement(<1..310)
                     /locus_tag="PL106_13605"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HNA34157.1"
                     /translation="MKELVCGIRPVEEALNSDVHVEKLMIRRDAGGAAIQNLQRLARE
                     REVPWQPVPLERLDRLTNAAHQGVIAWISAVATQDLDEVVQQAYDRGEDPLLIALDNV
                     T"
     gene            complement(360..>1394)
                     /locus_tag="PL106_13610"
     CDS             complement(360..>1394)
                     /locus_tag="PL106_13610"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNA34158.1"
                     /translation="ARVIIKYEAYPTIAGGTLLDSASTFVKDVSDTPKEDKDLIGSLD
                     MRRGDQRSFMLKVTALDLNRDTETTVFIPVETGEPGHRQGFLPMSERGIPLFDDNVPA
                     GMRVRILCEQYAGRTLVAERIKAPGKLPTPVFAPAGEKPIVEVDDTYSVTVGEDGSFP
                     FTADSTGLHYFRTDTSATEGYTLSTFNSTYPTVRTASDMVPPLRYITSLQEWNRMLTA
                     DDPRAEVEKFWTDAAGDRDRARAAIDAYYGRVESANRWFTSWTEGWRTDRGLVHIIFG
                     TPSTIHKGSTGEVWIYGEETNLMSLTFTFDKREGPFSDNDLTLRRDPNFKGAWYRNVE
                     AWRNGRVFQN"
BASE COUNT          208 a          449 c          435 g          302 t
ORIGIN      
        1 cggtcacgtt gtccaacgcg atcagcaacg ggtcttcgcc ccgatcgtag gcctgttgca
       61 ccacttcgtc aaggtcctgc gtggccaccg cgctgatcca tgcgatcacg ccctggtgcg
      121 cggcgttcgt cagccggtcc agccgctcca ggggtaccgg ctgccagggc acttcgcgtt
      181 cgcgggccaa gcgttgcagg ttctggatgg cggcgcctcc ggcatcgcgg cggatcatca
      241 gcttctctac atgcacatcg ctgttgaggg cttcttccac gggccggatc ccgcaaacga
      301 gctccttcat cgaagccggg ctggggcgcc cggccccgct gtgctgttgt ccccgcatgt
      361 cagttctgga agacacgacc gttgcgccac gcctccacgt tcctgtacca cgcccccttg
      421 aagttcgggt cacggcgcaa cgtgaggtcg ttgtcgctga atggtccttc gcgcttgtcg
      481 aaggtgaatg tgaggctcat caggttggtc tcctcaccat agatccagac ctcgccggta
      541 ctgcccttgt gtatggtgct cggcgtgccg aaaatgatgt ggaccagccc gcggtcggtg
      601 cgccagcctt cggtccatga ggtgaaccac cggttcgcgc tctccacccg gccgtaatac
      661 gcatcgatcg cggcacgtgc ccggtcacgg tcgccggcgg catcggtcca gaatttctcc
      721 acttcggccc gcggatcatc cgcagtgagc atcctgttcc attcctgcag gcttgttatg
      781 taccgtaatg gaggtaccat gtccgacgcg gtgcgcaccg tggggtaggt gctgttgaac
      841 gtggacagtg tgtatccttc ggtggccgag gtgtccgtgc ggaaataatg cagcccggtg
      901 ctgtcggcgg tgaaggggaa ggagccgtcc tcccccacgg tcacactgta cgtgtcgtcc
      961 acttccacga tcggtttctc gccggcgggt gcgaagaccg gggtcggcag cttgcccggt
     1021 gctttgatcc gctccgcgac cagggtgcga cccgcgtact gttcacagag gatccgtaca
     1081 cgcatgcccg ccggcacgtt gtcgtcgaaa agcgggatgc cccgctcgct catcggcagg
     1141 aagccttggc ggtggccggg ttctccggtc tctacaggaa tgaataccgt ggtttcggtg
     1201 tcgcggttca ggtccagggc ggttaccttg agcatgaacg atcgctggtc cccacgtcgc
     1261 atgtccaggc tgccgatcag gtccttgtcc tccttcggcg tgtcgctcac gtccttcacg
     1321 aaggtgctcg cactgtccag cagcgtgcca cccgcgatgg tgggatatgc ctcgtatttg
     1381 atgatgaccc tcgc
//
LOCUS       DAOQOC010000974         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_80245_length_1392_cov_3.489155, whole genome shotgun sequence.
ACCESSION   DAOQOC010000974 DAOQOC010000000
VERSION     DAOQOC010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80245_length_1392_cov_3.489155"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PL106_13615"
     CDS             complement(<1..761)
                     /locus_tag="PL106_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNA34159.1"
                     /translation="MTSREIRQKFLDHFARHGHTIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKDLFLGNAPIMHQRIADTQKCLRVSGKHNDLEEVGIDTYHHTMFEMLGNWSFGDYFK
                     KEAIQWAWELLVDEYKINKDDIYVTYFGGDEKDKLPADSETRDLWKRFMPEERILPFS
                     RKDNFWEMGDTGPCGPCTEIHVDVRTPEEKKVKSGRDLVNNDHPQVIEIWNNVFMQFE
                     RKADGSLVELPAQHVDTGMGFERLCMVLQGKRSNY"
     gene            867..>1392
                     /locus_tag="PL106_13620"
     CDS             867..>1392
                     /locus_tag="PL106_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HNA34160.1"
                     /translation="MAELKYRFNPDTLNFERIRLTLWQRVKRTGLALLPGLLLGVIVI
                     VVTFQIVDSPKEASLKRENEQLAVQYELMNKRLAEIEDVLADVQRRDDNVYRVIFEAD
                     PIPGTMRKAGVGGVRRYKDLEGYASSGLVMATRERLDRIAKQLYVQSVSLDEVAGLAI
                     RKQEMLAGIPSIQPI"
BASE COUNT          245 a          407 c          455 g          285 t
ORIGIN      
        1 tcgtagttgc tgcgcttgcc ctgcagcacc atgcacaggc gctcgaagcc catgccggtg
       61 tccacgtgct gcgccggcag ttccacgagc gagccgtcgg ccttgcgctc gaactgcatg
      121 aacacgttgt tccagatctc gatcacctgc gggtggtcgt tgttcaccag gtcgcgcccg
      181 ctcttcacct tcttctcctc cggcgtgcgc acgtccacat ggatctcggt gcaggggccg
      241 caaggtccgg tatcgcccat ttcccagaag ttgtccttgc gggagaaggg caggatgcgc
      301 tcctcgggca tgaaccgctt ccacaggtcg cgcgtctcgc tatcggcggg cagtttgtcc
      361 ttctcatcac caccgaaata ggtgacgtag atgtcgtcct tgtttatttt gtactcgtcc
      421 accagcagtt cccaggccca ctggatcgcc tccttcttga agtaatcgcc gaagctccag
      481 ttgccgagca tctcgaacat cgtatggtgg tacgtatcga tgccgacctc ctccaggtcg
      541 ttgtgcttgc ccgaaacgcg gaggcacttc tgcgtgtcgg cgatgcgctg gtgcatgatg
      601 ggcgcgttgc cgaggaagag gtccttgaac tggttcatgc ccgcgttggt gaacatgagc
      661 gtggggtcgt ccttgatcac catgggtgcg ctgggcacga tggtgtggcc gtggcgggcg
      721 aaatggtcga ggaacttctg gcggatctcc cgggaggtca tggtatctgg aaaaggagcg
      781 cgaaggtaga tgagtgtcgc ggcccctggg ggctgatgat cggcgcgtca ggtacggatc
      841 ctaccttcgt gcccggtcgc cgcaacatgg ccgaactcaa ataccggttc aatcccgaca
      901 cgctcaactt cgagcgcatc cggctcacgc tgtggcagcg cgtgaaacga acgggccttg
      961 cgttgttgcc tgggctgctc ctgggggtca tcgtcatcgt ggtcaccttc cagatcgtcg
     1021 attcgcccaa ggaggcatcg ctcaagcgcg agaacgagca gctcgccgtt cagtacgaac
     1081 tgatgaacaa gcggctggcg gagatcgagg atgtgctggc cgatgtgcaa aggcgggacg
     1141 acaacgtgta ccgggtgatc ttcgaagcgg atcccatacc cggcacgatg cggaaggcgg
     1201 gagtgggcgg ggtgcgtcgg tacaaggacc tggagggcta tgccagcagc ggcctggtga
     1261 tggccacgcg cgagcggctg gaccggatcg ccaaacagtt gtacgtgcag agcgtgtcgc
     1321 tcgatgaggt ggccggcctg gcgatccgca agcaggagat gctggcgggc atcccgagca
     1381 tacagcccat cc
//
LOCUS       DAOQOC010000975         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_80254_length_1392_cov_3.195961, whole genome shotgun sequence.
ACCESSION   DAOQOC010000975 DAOQOC010000000
VERSION     DAOQOC010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80254_length_1392_cov_3.195961"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PL106_13625"
     CDS             complement(<1..645)
                     /locus_tag="PL106_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34161.1"
                     /translation="MSHEQNTTDMDLKNLLAIALLMPFAANAQTDSTRTDTTHKRTYV
                     ALSVSKDGAHTYAGDGDSDGIDTFRLELKHRIIRIISTRRGTDSTGADTLEDRLRKAR
                     RERRNLFTYWAGIEFGFNSFIAPDGRIGDGPQAAPFLLNNLRGRWVAINIMEQKYEFG
                     SHHAGLFWGLGLEATNYHLSENVRLAYNSDSTWAVPVDDPRYTKNKLRQLGVRLP"
     gene            complement(768..1124)
                     /locus_tag="PL106_13630"
     CDS             complement(768..1124)
                     /locus_tag="PL106_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34162.1"
                     /translation="MKTTFTALALALATTGAIAATPFPGAPSPTTSMERELDRQLDKF
                     VTYPLLERTHLMDGEVLVSFVIDTEGRVKVISANSENTALCEYVLRMLAKVDIGDNPD
                     GLWKTTHVRFSFHPEA"
BASE COUNT          236 a          379 c          489 g          288 t
ORIGIN      
        1 cggcaggcgc acgcccagct gccggagttt gttcttggta tagcgcggat catccaccgg
       61 cacagcccag gtgctgtcgc tgttgtaggc cagtcgcacg ttctcgctca ggtggtagtt
      121 ggtggcctcc aggccgagac cccagaacag gcccgcgtgg tggctgccga actcgtactt
      181 ctgttccatg atgttgatgg ccacccagcg gccgcgcagg ttgttcagca ggaaaggtgc
      241 ggcttgcggc ccatcgccga tacggccatc gggagcgatg aaactgttga acccgaactc
      301 gatgccggcc cagtaggtga acaagttgcg acgctcgcgg cgggccttgc gcaaccggtc
      361 ctccaaagtg tcagcgccgg tgctgtccgt tccacgtcgg gtggagatga tgcggatgat
      421 ccggtgtttc aattccagac ggaaggtatc gatgccatcg ctgtcgccat cacccgcata
      481 ggtgtgcgct ccgtccttgc tcacgctgag cgcgacgtac gtccgcttgt gcgtggtgtc
      541 cgtgcgtgtg ctgtcggtct gggcgttcgc cgcgaagggc atcagcaggg cgatcgcgag
      601 gaggttcttc aggtccatgt ccgttgtgtt ctgttcatgg gacatggacg gtgggcggtt
      661 tgttacagcg ataggcggaa tgttgccggg gtacgaacgc agggatcccg gccgatcctc
      721 gcgaaggagg gtgcggccgg gatccatcgt cggataccga agagcgatca tgcctccgga
      781 tggaaggaga agcgcacgtg cgtggtcttc cacaatccat cggggttgtc gccgatgtcc
      841 accttggcca gcatgcgcag cacgtactcg cacagggcgg tgttctcgct gttggccgag
      901 atcaccttca cgcggccttc ggtatcgatc acgaagctca cgagcacttc gccgtccatg
      961 aggtgtgtgc gttccagcag cgggtaggtg acgaatttgt cgagctgccg gtcgagttcg
     1021 cgctccatgg aggtggtggg ggagggtgcg ccggggaacg gcgtggcggc gatggcaccg
     1081 gtggtggcca gtgcgagggc gagggcggtg aaggtggttt tcatgacatg aaggatcttt
     1141 tgttgtgcga cccgtttcct gttgtcggtt gttcagccgt aagacgccac cgggccctcg
     1201 gatgttaccg ttcacgggtc gctcttcgat gagcggtcaa cggccgggga tgagcggtaa
     1261 gccttgcggg ccgggcgcca agcaaaggcg gttccggcca gtgtgaagct gaaggaaccg
     1321 gaagaaatac caagaaacgt gaaaacccgg tgacggatgg ttcagttcgt ggtgctcgca
     1381 gcgatggcca gt
//
LOCUS       DAOQOC010000976         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_80392_length_1391_cov_2.805389, whole genome shotgun sequence.
ACCESSION   DAOQOC010000976 DAOQOC010000000
VERSION     DAOQOC010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80392_length_1391_cov_2.805389"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1391
                     /locus_tag="PL106_13635"
     CDS             <1..>1391
                     /locus_tag="PL106_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34163.1"
                     /translation="AYDMIIGEDTLHGSEAGFVAKHDAEGDYVWSVNCVAPTGTMSVS
                     AMTLDTANDVLIIAGGHSRTSMIGNFQLPDSGAYLAWISVNGVCLNAQNIGSPRTGVS
                     ALALHDEDICIAGVTSDGQSLQIGGQSIQPGSFIARFHTDGTPVWAKNMILGFSSQNV
                     RYFPSKILSSGSDLLVFGTAMQQPEAGTFDMDTVHVNVNAGGGCALISMDSETGSAQW
                     IRLLGYGPTGGWSPTIKNVIDVDDHGNAYVTLTVSGDSVHFAPDTVVHVPSGHSLPCL
                     VKYTPAGELSTFRTFGLDAGFSALSKEESGTLLMMASFLDSSSVDVCSSQDGTWLVRL
                     DTSGNCLGSVPVALNTPPWQDDASGLSIYPTPDGIYLSAATPYSPQFSTITFAGESVT
                     SHGWEDVLLAKCALSLGVGTKSMEDTEQLLIYANPNNGSFRIQVPDALVHERQLLLSI
                     HDGSGRMVYQAPL"
BASE COUNT          301 a          370 c          385 g          335 t
ORIGIN      
        1 aggcctatga catgatcatt ggtgaagata cactccatgg ttccgaggcc ggttttgttg
       61 ccaagcatga tgccgaaggc gattacgtat ggtcagtcaa ttgcgtggca cccacaggca
      121 ccatgagcgt ttccgccatg accttggaca cggcgaatga tgtactcatc attgccgggg
      181 ggcattcgcg aacatcaatg atcggaaatt tccaattacc ggacagcggc gcctatctgg
      241 cttggatcag tgtgaacggc gtatgcctga atgcccaaaa tattgggtca ccccggaccg
      301 gcgtgagcgc cttggcactt catgatgagg atatctgcat tgctggggta acaagtgatg
      361 gacagtcctt gcaaataggt ggccaatcca tccagcccgg gtctttcatc gcgcggttcc
      421 acactgatgg taccccggtg tgggctaaga atatgatcct tggcttttca tcacaaaatg
      481 tccgctactt cccgtcgaaa attctatcat ccggttccga ccttttggta ttcggaacgg
      541 ctatgcagca gcccgaagct ggaacatttg atatggacac ggtgcatgta aacgtcaatg
      601 caggtggagg atgtgcattg atttccatgg atagtgaaac aggatccgct caatggatcc
      661 gattgctggg gtatggccca actggtggct ggtccccaac gataaagaat gtgattgatg
      721 ttgatgacca cggaaacgcc tatgtgacgt taaccgtctc tggggattcc gttcatttcg
      781 cccctgacac cgtggtacac gtaccatcag gccattccct gccctgcttg gtcaagtata
      841 ctccggcagg cgagctgtct accttccgaa cattcgggtt ggacgctggt ttcagcgcgc
      901 ttagtaagga ggaaagtggc acgctgctga tgatggccag tttcttggat tcctcctcag
      961 tggatgtttg ttcctcccag gatggcacgt ggctggttcg cttggatacc tcgggcaatt
     1021 gtcttgggtc cgtaccagtg gctttgaaca cgcccccttg gcaggatgat gcctcgggtc
     1081 tcagcatcta tccaacaccg gacggcatct acctcagtgc ggccacacca tattccccgc
     1141 agttcagcac catcaccttc gccggtgaga gcgtaacgag ccatggctgg gaggacgtgt
     1201 tactggccaa gtgcgcattg agcttgggcg tgggcaccaa gagcatggag gataccgaac
     1261 agttgctcat ttacgccaac cccaacaacg gtagcttccg catccaagtg ccggatgcct
     1321 tggtgcatga acggcaattg ctgctgtcca tccacgatgg cagtggccgc atggtgtacc
     1381 aggcaccact g
//
LOCUS       DAOQOC010000977         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_80740_length_1388_cov_3.772693, whole genome shotgun sequence.
ACCESSION   DAOQOC010000977 DAOQOC010000000
VERSION     DAOQOC010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80740_length_1388_cov_3.772693"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..524
                     /locus_tag="PL106_13640"
     CDS             72..524
                     /locus_tag="PL106_13640"
                     /inference="COORDINATES: protein motif:HMM:NF016961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNA34164.1"
                     /translation="MKYKTYLLQLFDPSGGNIVAQAGGIAIATVASFVMPVKGYLFLI
                     GGLVMVDLYTGWRKSKKETGAKLNSAGLGKTLEKSGLYLLLMLVSRGVDKTYGLDGTL
                     SLSWFVGGLVTGREVLSIFENADAVLGTNFSHRIRDVWQRITGSKPVE"
     gene            524..961
                     /locus_tag="PL106_13645"
     CDS             524..961
                     /locus_tag="PL106_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34165.1"
                     /translation="MLDEKSVELLLRCDVRLANIITMASDVSDVRFRVTETARTVENQ
                     REYYRKGVSKVNPDAYTELASLYKAAKHIVGPGMPKSRAVDIAIVGKEPYHVASLSYV
                     AGVVATIAKSKGTPVRWGGDFDRDGVIVEAGTFQDLPHFELDL"
     gene            968..1300
                     /locus_tag="PL106_13650"
     CDS             968..1300
                     /locus_tag="PL106_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34166.1"
                     /translation="MRAIQYLNLALAAVLVLAIAYNFLRGWNAGVDDKAYDRMWRAER
                     ERDSVAREAYRWRTEAEGYERRADSLAALVDSAKAVLPPMAEVREAKVKALKYAPLNA
                     VIDTLAKE"
BASE COUNT          274 a          324 c          522 g          268 t
ORIGIN      
        1 tgtcaatcca ccgtgggggc gaatatagtg catgtatcgg aattggatag cggtgctatg
       61 tttgcggggt gatgaaatac aagacctatc tcttgcagtt gttcgacccg agcgggggga
      121 acatcgtggc ccaagctggt ggcatagcca tcgccaccgt ggcatccttc gtgatgccgg
      181 tgaagggcta cctgttcctc atcggggggt tggtgatggt ggacctttac acgggctggc
      241 ggaagtccaa gaaggagaca ggggcgaagc tgaacagcgc gggtctgggc aagacccttg
      301 agaagtccgg actctacctg ctgcttatgc tcgtgtcgcg aggggtggac aagacctacg
      361 ggctggacgg tacgctgtcc ttgtcgtggt tcgtcggtgg gcttgttacc ggacgtgagg
      421 tattgagcat cttcgagaac gcggacgcgg tgctggggac gaacttcagt catcggatac
      481 gcgacgtgtg gcagcgcatc accggcagca agcccgtgga gtgatgctgg acgagaagag
      541 cgtggagttg ctgttgcgct gcgacgtgcg gctggcgaac atcatcacca tggccagcga
      601 cgtgagcgat gtgcggttcc gtgtgacgga gacggcgcgc acggtggaga atcagcgtga
      661 atactaccgg aagggggtga gcaaggtgaa cccggacgcc tatacggaac tggcgtcttt
      721 gtacaaggcg gcgaagcaca ttgtagggcc tgggatgccc aagagccgtg cggtggacat
      781 cgcgatcgtt gggaaggagc cttaccacgt ggcgagtttg agttatgtgg ctggggtggt
      841 cgcgacgatc gccaagagca aggggacgcc ggtgaggtgg ggaggggact ttgaccggga
      901 cggcgtgatc gtggaggctg ggacgttcca ggacctgccg cacttcgagt tggatctttg
      961 atataccatg agggccatac aatacttgaa ccttgcgctg gcggcagtgc tcgtgctggc
     1021 catcgcctac aactttctgc ggggctggaa tgccggggtg gatgacaagg cgtacgaccg
     1081 gatgtggcgt gcggagcgtg agcgggacag cgtggcgcgg gaggcgtacc ggtggcggac
     1141 agaggcggaa ggatacgaac ggagggcgga cagccttgcg gcgctggtgg acagcgcgaa
     1201 ggcggtgctg cccccgatgg cggaggtgag ggaggcgaag gtgaaggcgc tgaagtacgc
     1261 gccattgaac gcggtgatcg acacgctggc caaggaatga ggctgattgc tgccatagct
     1321 gtgatgctgt gctttgatgt acaaggccag gtggacagct atggcagcaa tcagcctcat
     1381 tccttggc
//
LOCUS       DAOQOC010000978         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81017_length_1386_cov_2.560481, whole genome shotgun sequence.
ACCESSION   DAOQOC010000978 DAOQOC010000000
VERSION     DAOQOC010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81017_length_1386_cov_2.560481"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PL106_13655"
     CDS             complement(<1..1105)
                     /locus_tag="PL106_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34167.1"
                     /translation="MIELLVLDSLEGTPLEAVSVAWTDGTRAMAQLTDPHGRCAIAWP
                     GSGPLELRLARAGYRPRSMGLDPDDARGRRPFTVYLAALELPAVEVVRKPEVVFERPD
                     LHAADLLINDDGIWVLAYEHPRLLRAEVDAGKEILRGVRLVLLDTNFREWASCPLPED
                     VLGLRRDLRNTALVEGVSRAFAIHRAGDHLELEPFGLEDLRSKVLPWTDSIGGRILGN
                     NSDGVMPAFDHLAYDPVNDTTRVVCTVMDTFMMALFRSEYRQLRGPEKVLAMNLASEL
                     GVDEETVAGYMSGFQHNIWFKPVYAPLFVAGDTLLVFDHASARLRKFTRGLVETASVP
                     LPYLAEQGGRYRPMRIIQDRADGKLFALFQHNGI"
BASE COUNT          279 a          462 c          417 g          228 t
ORIGIN      
        1 ggatgccatt gtgctggaaa agggcgaaca gctttccgtc cgcccgatcc tgaatgatac
       61 gcatcggccg gtaacgccct ccctgttcag cgaggtaggg caagggcacg ctggcggtct
      121 ccacaagtcc acgcgtgaac ttgcggaggc gggcggaggc atgatcgaaa acgagcaagg
      181 tgtccccggc cacgaaaagc ggtgcgtaca ccggcttgaa ccagatgttg tgctggaacc
      241 cgctcatgta tcccgccacg gtctcctcat ccacgccgag ttccgatgcg aggttcatgg
      301 ccagtacctt ctcggggccg cgaagttgcc ggtactcgct gcggaaaagc gccatcatga
      361 aggtatccat cacggtgcag accacacggg tcgtatcgtt cacggggtcg taggccaggt
      421 gatcgaaagc gggcatcacc ccgtcggaat tgttgcccag gatacggccg ccgatgctgt
      481 ccgtccacgg gagcaccttg gagcgcaggt cctccaggcc gaagggttcc agttccaggt
      541 gatcgcccgc acgatgtatg gcgaaggcgc gcgaaacacc ttcaacaagg gcggtattcc
      601 gcagatcgcg gcgcaggccc agcacatcct cgggcaatgg gcagctggcc cattcccgga
      661 aattggtatc gagcagcacc aggcgcacac cgcgcaggat ctccttgccc gcatcgacct
      721 cagcgcggag caggcgcgga tgctcgtagg ccagcaccca gatcccgtca tcattgatca
      781 acaggtcggc cgcgtgcaga tcggggcgtt caaagaccac ttcgggcttg cgcaccacct
      841 ccacggcagg cagttccagt gccgcgagat acacggtgaa tggccgccta ccgcgcgcat
      901 catccggatc caggcccatg gaccttggcc ggtaacccgc acgggcgagc cgcaactcca
      961 acggaccact tcccggccaa gctatcgcac agcgaccatg tggatcggta agctgtgcca
     1021 tggcgcgtgt accatcggtc cacgccacac tcacggcctc cagcggcgtg ccctccaagc
     1081 tgtccagcac gagcagttcg atcacgctcg tctgagccga aaggggaagc accgtcaggc
     1141 aaagccccca tgccgcatac cgcaaccgca tcactggccg ttcctacttt tacgatgcca
     1201 agatggggcg gatcgcacac gcccgcctac caccacggat gcaccaggca caggagaacg
     1261 atctgtatgg caccttgggc gttccgcgct cggcggatgc ggtcacgatc aagcgtgcgt
     1321 tccgcgcgca tgccaaacgc tgtcatccgg acgtggaccc ctcgcccatg gcgggcgtcc
     1381 gtttca
//
LOCUS       DAOQOC010000979         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81341_length_1383_cov_3.165663, whole genome shotgun sequence.
ACCESSION   DAOQOC010000979 DAOQOC010000000
VERSION     DAOQOC010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81341_length_1383_cov_3.165663"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PL106_13660"
     CDS             <1..539
                     /locus_tag="PL106_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNA34168.1"
                     /translation="GENGVHRLVRISPFDSNARRHTSFVSVYVYPLVDESIEIDINPA
                     DITWDTFRSGGAGGQNVNKVETGVRLRHAPSGIIIENTETRSQLDNKNKALQLLKSQL
                     YEMELERRRAKRSEIEAGKKKIEWGSQIRNYVLQPYKLVKDVRTEHETSNVDDVLNGY
                     IDEFLKAYLMMHGQSQKV"
     gene            936..>1383
                     /locus_tag="PL106_13665"
     CDS             936..>1383
                     /locus_tag="PL106_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNA34169.1"
                     /translation="MDHRIEKDTMGEVKVPADKYWGAQTERSRNNFRIGPPASMPREI
                     VHAFAYLKKAAALTNLELGKLPKEKCDLIGRVCDEILEGKLDEQFPLVIWQTGSGTQS
                     NMNVNEVVAYRAHVLQGGKLTDEQKVLNPNDDVNKSQSSNDTYPTAM"
BASE COUNT          349 a          395 c          443 g          196 t
ORIGIN      
        1 agggcgagaa cggtgtgcac cgcctggtgc gcatcagccc cttcgacagc aacgcgcggc
       61 ggcacacgag cttcgtgagc gtgtacgtgt acccgctggt ggacgagagc atcgagatcg
      121 acatcaatcc ggccgacatc acctgggaca ccttccgcag cggcggcgcc ggcgggcaga
      181 acgtgaacaa ggtggagacc ggtgtgcgcc tgcgccacgc acccagcggt atcattatcg
      241 agaacaccga gacccgcagc cagttggaca acaagaacaa ggcgctgcaa ctgctgaagt
      301 cgcagctcta tgagatggaa ttggaacgcc gccgtgccaa acgcagtgag atcgaagcgg
      361 gcaagaagaa gatcgagtgg ggcagccaga tccgcaacta tgtgctgcag ccctacaagc
      421 tggtgaagga cgtgcgcacc gagcacgaga cgagcaacgt ggatgatgtg ctgaacggat
      481 acatcgatga gttcctgaag gcttatctga tgatgcacgg acagagccag aaggtgtgaa
      541 tgagggagcg atgccggact ggaccatcta ccacaacgcc gagtgcagca agagcaactg
      601 tgcccttgac ctgctccgcg cgagcggagt ggaaccccgc gttgtgaact acctgagcga
      661 aacaccttcg atggaggacc tgtcccgggt gctggctgca ctcggcatcc ctgccgaaca
      721 actcgtgcgc aagagcgagg ccctgtacaa ggagaagtac gcgggtcttc agatgaagga
      781 gaacgaatgg atacgggtga tgcacgagca ccccgagctg atcgagcgcc ccattgtact
      841 gaaggggcga caaggccgtg atcggacggc ccgtggagaa cgtacaggaa ctcatcaacc
      901 aatagcaagg gcgcgggatc tagcacccaa caggaatgga ccaccggatc gagaaggaca
      961 cgatgggcga ggtgaaggta cccgccgaca agtattgggg cgcacagacc gagcgcagtc
     1021 gcaacaactt caggatcggg ccgccggcga gcatgccgcg tgagatcgtg catgccttcg
     1081 cctacctgaa gaaggccgct gccctcacca acctggaact gggcaagctg cccaaggaga
     1141 agtgcgacct catcggacgc gtgtgcgacg agatcctcga aggcaagctc gatgaacagt
     1201 tcccgctggt gatctggcag accggcagtg gcacccagag caacatgaac gtgaatgagg
     1261 tggtggccta tcgcgcgcac gtgctgcagg gcggcaaact caccgatgag cagaaggtgc
     1321 tcaacccgaa cgacgatgtg aacaagagcc agagcagcaa cgatacctac cccaccgcca
     1381 tgc
//
LOCUS       DAOQOC010000980         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81448_length_1382_cov_3.474755, whole genome shotgun sequence.
ACCESSION   DAOQOC010000980 DAOQOC010000000
VERSION     DAOQOC010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81448_length_1382_cov_3.474755"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1330)
                     /locus_tag="PL106_13670"
     CDS             complement(386..1330)
                     /locus_tag="PL106_13670"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNA34170.1"
                     /translation="MSIDMLPHALMRSDLRLRDLEAVYRGKVRDVYHLRDGRTILVAT
                     DRISAFDVVLPRGIPHKGQVLTQVSWHMLETTRHTAPNWAIASPDPCVVVGHRATTVK
                     VEMVVRGYLSGHAWRQYCEGHRVLCGAEMPDGLRESDPFPQPIITPSTKADEGHDEDI
                     TPQAILERGLCTREEWATMSRYALALFAEGTRIANERGLILVDTKYEFGRLPNGDIIL
                     IDEIHTPDSSRYFYRDGYAERQDRGEPQQQLSKEFVRQWLMSNGFMGQEGQVMPEMTD
                     TYVKSVSDRYIELYERITGRGFVPADTSDIAARIQAAL"
BASE COUNT          237 a          427 c          435 g          283 t
ORIGIN      
        1 ctccggcgac ctctgatgac cctcatgaga agtacgcaga tgtgtccagg ttcaacccgg
       61 cggccagagg tcgaagaacc tcggcactcg ggaaacaccc gagcgccgat tgaggtatct
      121 ttatgaactg tcacgctgag cgcgccgaag cgctatacgc cacagaacgg tccagactcg
      181 atctgttgat ccaatgcgcc acaactgatt ggtgagcgct tcggcaggct cagcgtgaca
      241 atgactggga gcgtgcatgg cattggaata gcgcttcggt ccttccttcg ccaagaaagg
      301 atctctcagc gtgaccgtgt atggggagcg cgtcgaggcg caataccctt tcaggcacgt
      361 tacgatcgcg tcaccccagg tacccttaca acgctgcttg gatcctggca gcaatgtcgc
      421 tggtgtccgc cggtacgaaa ccgcgcccgg tgatgcgctc gtacagttcg atgtaacggt
      481 cgctcacgct cttcacatag gtgtcggtca tctcgggcat gacctggccc tcctgcccca
      541 tgaagccgtt gctcatgagc cactggcgta cgaattcctt gctcagttgc tgttgcggct
      601 cgccccgatc ctgccgttcg gcatagccgt cccggtagaa atagcgcgaa ctgtccggcg
      661 tgtggatctc gtcgatcagg atgatgtcgc cattcggcag gcggccgaat tcgtacttgg
      721 tgtccaccag gatcaatccg cgctcgttgg cgatgcgcgt gccttcggcg aagagggcga
      781 gtgcgtaccg gctcatggtg gcccactcct cccgggtgca aagtccgcgc tccaggatgg
      841 cctgcggggt gatgtcctcg tcgtgcccct cgtcggcctt ggtgctgggg gtgatgatgg
      901 gctgcggaaa gggatcgctc tcgcgcaggc catcgggcat ttcggctccg cagagcacgc
      961 ggtggccttc gcaatactgc cgccaggcat gaccgctgag gtaaccgcgc accaccatct
     1021 ccaccttcac ggtggtggca cggtgaccta ccaccacgca gggatcgggc gaggcgatgg
     1081 cccagttggg cgctgtgtgt cgggtggttt ccagcatgtg ccagctcacc tgggtgagca
     1141 cctggccctt gtgcggaatg ccgcgcggta gcaccacgtc gaatgcgctg atccggtcgg
     1201 tggccaccag gatggtgcgg ccatcgcgca ggtggtacac gtcgcgcacc ttgccccggt
     1261 atacggcctc caggtcgcgc aaccgcaggt cgctgcgcat gagcgcgtgg ggaagcatgt
     1321 cgatgctcat ttatcggtat tgtcggtccc gccttcgatg tccttgaagg ccgatagcac
     1381 cc
//
LOCUS       DAOQOC010000981         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81483_length_1382_cov_2.811605, whole genome shotgun sequence.
ACCESSION   DAOQOC010000981 DAOQOC010000000
VERSION     DAOQOC010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81483_length_1382_cov_2.811605"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PL106_13675"
     CDS             complement(<1..1162)
                     /locus_tag="PL106_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34171.1"
                     /translation="MRCSFLVYPALLLVFLHTETCLAQRPFTQANGDCTGAIPILDSV
                     YVCEQAVRGFGNVLEIKENPADQKEWFEREHNTTWYTFRAPVKTIITMDIIPMNIEDD
                     IDFLIFEGAVPGICDKIQNKQVVPLRTNISRNDKMNGSICGLRKDAPDKYVRSGIGSS
                     FSQGLDVEAGQLFYLVIDYQDRPLDGYTIRFHYDPPPPPPPAPDEKQQELSIRITDAK
                     TGAPLEADLAIEGMVFDSVVEAKGRSAYDLKMDPYRNIKIGCIRPGYMFYSQKVKGTT
                     APVVEVQVKLIPIEAGEHVVLEDIHFVGNDVKVMRQSEGSLLLLVRFMLANPRVKVEV
                     QGHVNGPTYKNTKEFIELSTGRARTVYDFLMLNDVEPDRVTYVGLGNSQMLYP"
     gene            complement(1196..>1382)
                     /locus_tag="PL106_13680"
     CDS             complement(1196..>1382)
                     /locus_tag="PL106_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNA34172.1"
                     /translation="EGLAMWPAAAVSGLYFAHPRSKYFGIGRVAKDQIEDLARRKGQE
                     TAWMERWLGSNLGYDPA"
BASE COUNT          249 a          430 c          407 g          296 t
ORIGIN      
        1 cgggatacag catctgactg ttgcccaggc ccacgtaggt cacgcggtcg ggctccacat
       61 cgttgagcat gaggaagtcg tacaccgtgc gggcacgtcc ggtgctcagc tcgatgaact
      121 ccttggtgtt cttgtacgtg ggcccgttca catggccttg gacctccact ttcacccgcg
      181 ggttggccag catgaaccgc accagaagca gcaatgaacc ttcgctctgg cgcatcacct
      241 tcacatcatt gcccacgaag tggatgtcct ccagcaccac gtgctcacca gcttcgatcg
      301 ggatcagctt cacctggacc tcaacgaccg gcgcggtggt ccccttcacc ttctgcgagt
      361 agaacatgta accgggcctg atgcagccga tcttgatatt ccggtacggg tccatcttca
      421 gatcgtatgc gctgcggccc ttggcctcca cgaccgagtc gaaaaccatg ccctcgatcg
      481 ccaagtcggc ctccaatggt gcacctgtct tcgcgtcggt gatgcggatg ctcagttcct
      541 gctgcttctc gtccggcgct ggtggtggag gtgggggcgg gtcgtaatgg aaacggatcg
      601 tgtagccgtc cagggggcgg tcctgatagt cgatgacgag gtaaaacaac tgcccggcct
      661 ccacatcaag tccctggctg aaagacgagc cgatacctga tcgcacgtat ttgtccgggg
      721 catccttccg caggccgcag atgctgccgt tcatcttgtc gttccgactg atgttggtgc
      781 gcagggggac cacttgcttg ttctggatct tgtcgcagat gcctggcacg gcaccctcga
      841 agatcaggaa gtcgatatca tcctcgatgt tcatcgggat gatgtccatg gtgatgatgg
      901 tcttcaccgg cgcccggaag gtgtaccagg tggtgttgtg ctcacgctcg aaccattcct
      961 tctggtcggc cggattctcc ttgatctcga gcacattgcc gaagccacgg accgcctgtt
     1021 cgcatacgta caccgagtcg aggatcggta tggcacccgt acagtcgcca ttcgcttgcg
     1081 tgaacggacg ttgtgcaagg caggtctccg tgtgcaggaa taccaggagc agcgcgggat
     1141 atacgaggaa ggagcacctc acgggaagag catggacact tggcccgagc cccttctacg
     1201 caggatcgta ccccaggttg ctgcccaacc accgttccat ccacgccgtc tcctggccct
     1261 tgcgccgcgc caggtcctcg atctggtcct tggccacgcg gccgatgccg aagtacttgc
     1321 tcctggggtg ggcgaagtag aggccgctca ccgccgcagc gggccacatg gccaggcctt
     1381 cg
//
LOCUS       DAOQOC010000982         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81812_length_1379_cov_3.666918, whole genome shotgun sequence.
ACCESSION   DAOQOC010000982 DAOQOC010000000
VERSION     DAOQOC010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81812_length_1379_cov_3.666918"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1379
                     /locus_tag="PL106_13685"
     CDS             <1..>1379
                     /locus_tag="PL106_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34173.1"
                     /translation="TLRLAAIGSTLLTLKAMALVPLPVPLAPPAAPPGENIITCGAPP
                     LDETYCYGPSDSQSWLYTSSDGTTSLALIFSAGQIESDNWDHLTIYDGPDNSYPILFT
                     HAGVFSTQDLTGVLAISTGPNIFMQMSSDASVSCSTGSFTSWAWQVGCLDCVQPQATF
                     AVDLDCALGIFNVVVDVTAMGSDPSIDITNTGGAPVVTATATGSYTVGPFAQGTTVTV
                     VLENELNSLCNVSSGPLTNYPCPIISCGPDQYSYCYGNSETFIQIFQGNNGFPVRLQF
                     NSGGIYQFDGDLLTIYDGLNDQAPIIYQGTGTNGDLSGMFFTSSNPDFALTLKLTSTQ
                     FTSCSDGIALPWDYVVGCLDCVPPTATAGQVTTDCDGQIFSISVEISDLGTDPQVEIA
                     NDQGYPPTVVSAPGSYTAGPFPVGVPITLTVVNDANALCNVDLGVFENAFCPLIIPCA
                     NPALDQSYC"
BASE COUNT          262 a          445 c          383 g          289 t
ORIGIN      
        1 tacgttacgc ttggccgcga taggctccac cctgctcacc ctcaaggcca tggccctggt
       61 gcccttgccc gttccgctgg caccaccggc agcgcccccg ggggagaaca tcatcacctg
      121 tggtgcgcca ccgctggatg agacctattg ctatggcccc agcgattcgc agagctggct
      181 gtacacgagt tctgatggga ccacctcact ggcgcttatt ttcagtgcag gccagatcga
      241 aagcgacaac tgggaccacc tcacgatcta cgatggtccc gacaactcat acccgatcct
      301 gttcacacac gcgggtgtct tcagcacgca ggacctcaca ggtgtgctgg ccatcagcac
      361 cggcccgaac atcttcatgc agatgagttc ggatgccagc gtgtcgtgct ccacgggcag
      421 tttcacttcc tgggcatggc aggtgggctg tctggactgc gtacagccac aggccacgtt
      481 cgctgtcgat ctggactgcg cacttgggat cttcaatgtg gtggtggatg ttaccgccat
      541 gggatccgat ccgtcgatcg acatcaccaa cacgggcggt gcgccggtgg taacggccac
      601 cgcaacgggc tcctacaccg tgggtccctt cgcacaagga accacggtaa cggtggtcct
      661 ggagaatgaa ctcaactccc tgtgcaacgt gagctcgggc ccgctgacca actacccctg
      721 tccgatcatc agttgtggtc cggatcagta ttcatactgc tacggaaatt ccgagacctt
      781 catccagatc ttccagggga acaacgggtt cccggtgcgc ctccagttca acagcggtgg
      841 catctaccag ttcgatgggg acctgctcac catttacgat ggcttgaatg atcaagcccc
      901 gatcatctat cagggaacgg gcaccaatgg tgacctcagc gggatgttct tcacctcctc
      961 caaccccgat ttcgcactca cgctcaaact gacctccacc cagttcacca gttgttccga
     1021 tggcatcgcg ctgccgtggg attacgtggt gggctgcctc gattgtgtgc cgcccacggc
     1081 cacggccggc caggtgacca ccgattgcga cggacagatc ttctcgatct ccgtggagat
     1141 ctccgacctc gggaccgatc cacaagtgga gatcgcgaac gatcagggct atccgcctac
     1201 cgtggtgagt gcgccgggat cgtacaccgc aggtcctttc ccggtggggg ttccgatcac
     1261 cctcaccgtg gtgaacgatg ccaacgcgct ctgcaacgtg gatctggggg tgttcgagaa
     1321 cgccttctgc ccgttgatca tcccttgtgc caacccggcc ctggatcaga gttactgct
//
LOCUS       DAOQOC010000983         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81908_length_1378_cov_7.204082, whole genome shotgun sequence.
ACCESSION   DAOQOC010000983 DAOQOC010000000
VERSION     DAOQOC010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81908_length_1378_cov_7.204082"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1378
                     /locus_tag="PL106_13690"
     CDS             <1..>1378
                     /locus_tag="PL106_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34174.1"
                     /translation="ATVTVTQNDPVNAGSDGAITVCADGATLDLFPLLTGAESGGTWT
                     DPNNAASTSTYTPGTSVPGVYTYSLTALAPCTNDAATVTVTQNDPVNAGSDGAVTLCV
                     SSPVTDLFAVLGGTPDAGGSWTAPDGTAHTGAFTPGLDASGAYTYSLSAIPPCTNASA
                     TVTVAVVDLPDPGSSGAITLCLSGTTADLFGMLGGTPDAGGLWTAPDGTSHPSTVDPS
                     ADQAGVYTYTIDVPPPCVSVSSTVTLSLQTPPDAGLNGTSLLCVTSPPIDLFLELAGT
                     PEAGGTWTAQDGSTSDGTFTPGTSTPGIYTYTVPGIAPCPLAVSNVEVTVTSIPDAGS
                     PGSATLCMVGPTQELFDLLGGTPDQGGNWTAPDGVSHNGTFDPNMDPPGVYVYTLNAP
                     LPCPTVSSSVTLTVNEPPDPGVDGSLLLCISSPPSDLFNSLGGAPDMNGSWSDPGGNT
                     FSGTFDPGM"
BASE COUNT          270 a          468 c          384 g          256 t
ORIGIN      
        1 tgccacggtc accgtcaccc agaacgatcc cgtgaatgcg ggaagcgatg gtgccatcac
       61 cgtatgtgcc gatggcgcga ccctggacct cttccccctg ctcactggcg cggaaagcgg
      121 cggaacgtgg accgacccca acaacgcagc gagcacctcc acctacactc cgggcaccag
      181 cgttccgggg gtgtacacct attccctcac cgccctcgcg ccctgcacga atgacgctgc
      241 cacggtcacc gtcacccaga acgatcccgt gaatgcggga agcgatggtg ccgtaacgct
      301 ttgtgtctcg agtccagtca ccgacttgtt cgccgtattg ggcggcacgc ctgatgctgg
      361 tggctcatgg acagctcccg atggtaccgc ccacaccggc gcgttcacgc ccgggctcga
      421 tgcgtcgggc gcctacacct attcattgag cgcgatccca ccttgtacca atgcaagtgc
      481 caccgtgacg gtagccgttg tggacctgcc cgaccctggc tcaagcggag ccattaccct
      541 atgtctctca ggcacaaccg cggatctgtt cggcatgctg ggtggtaccc cggacgctgg
      601 cggcctttgg actgcgcccg atgggacctc gcacccgagc accgtggatc cttccgcgga
      661 tcaagccggt gtgtacacat acaccattga tgtgcccccg ccctgtgtga gcgtgagcag
      721 tacggtaaca ctatcacttc aaactccacc ggatgccggt ttgaacggta cgtcgctcct
      781 ctgcgtgacc agtcccccga tcgacctctt cctcgaattg gcaggaacac cggaggcggg
      841 tggcacctgg accgcacaag acggaagcac atccgatggc acattcacac ccggaacgag
      901 tacgcccgga atatatacat atactgttcc tggtatagcg ccctgtccgt tggccgtttc
      961 caacgtggaa gttacggtaa cctcgatccc tgacgccgga agtcctggat ccgcgacctt
     1021 gtgcatggtg ggaccgacac aggagctttt cgacctgctc ggcggcacac cggaccaggg
     1081 cggcaattgg accgcacccg atggcgtcag ccacaacgga acattcgacc cgaacatgga
     1141 tccaccgggt gtgtacgtct acacgctcaa cgcacccctg ccctgcccta cggtcagcag
     1201 ttcggtgaca ttgaccgtga acgaaccgcc cgatccaggt gtcgatggat ccctcctgct
     1261 ctgtatctca agcccaccga gcgacctgtt caactccctc ggtggcgcgc cggacatgaa
     1321 cggtagttgg agcgatccgg gaggaaacac tttcagcgga accttcgacc caggaatg
//
LOCUS       DAOQOC010000984         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81931_length_1378_cov_3.405896, whole genome shotgun sequence.
ACCESSION   DAOQOC010000984 DAOQOC010000000
VERSION     DAOQOC010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81931_length_1378_cov_3.405896"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /gene="ric"
                     /locus_tag="PL106_13695"
     CDS             <1..882
                     /gene="ric"
                     /locus_tag="PL106_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNA34175.1"
                     /translation="RYHALKKGESLVIHNDHDPKPLYYQMQGEMGDVFTWEYLEQGPA
                     WWRVKITKRPDGTGAETLAEIVTKDFHKAEVLKKYGLDFCCNGHLTMREACAAKGLDV
                     VRIEQELREADKVLTAPALPYNDWTIDFLADFIVNTHHAFLRKNLPEIGKYSAKVAQV
                     HGAAHPELHEVNGLVEEVIAEFTAHLPEEEKNMFQYIKRMVTARNAGQPRPASTMGRF
                     QDHLESLVNEHVSVGGKLDRINELTDGYTLPGDACASYTLLFRLLRELEDDTHVHIHL
                     ENNILFPKTAELERSFN"
     gene            1002..>1378
                     /locus_tag="PL106_13700"
     CDS             1002..>1378
                     /locus_tag="PL106_13700"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNA34176.1"
                     /translation="MTITSDTKIAALLRESPDALEAIISLSPKYNKLRNPLLRKLMAS
                     RATIGMASRIGGRTPEDFFRVLEPLGFVVDRSASTGGEEPRTPRPAFMERLSPEQVQD
                     LDVRPILNAGKDPFNLILGKVQA"
BASE COUNT          288 a          457 c          413 g          220 t
ORIGIN      
        1 cgctaccatg cgctgaagaa gggcgaaagc ctcgtcatcc acaacgacca cgaccccaaa
       61 ccgctctact accagatgca gggcgagatg ggcgacgtgt tcacctggga atacctggaa
      121 caaggcccgg catggtggcg tgtgaagatc accaagcgac ccgacggcac cggtgcggag
      181 accctggcgg agatcgtcac caaggacttc cacaaggccg aggtgctcaa aaaatacgga
      241 ctcgacttct gctgcaacgg ccacctcacc atgcgcgagg cctgcgcggc caaagggctg
      301 gacgtggtgc ggatcgagca ggagctgcgc gaagcggaca aggtgctgac cgccccggcc
      361 ctgccgtaca acgactggac catcgacttc ctcgcggact tcatcgtgaa cacgcaccac
      421 gccttcctgc gcaagaacct tccggagatc gggaagtatt ctgccaaggt ggcacaggtc
      481 catggtgccg cgcatcctga actgcacgag gtgaacgggc tggtggaaga ggtgatcgcg
      541 gagttcaccg cccacttgcc ggaggaggag aagaacatgt tccagtacat caagcggatg
      601 gtgacggccc ggaatgccgg ccagccgcgg ccagccagca ccatgggccg cttccaggat
      661 cacttggagt ccttggtgaa cgagcatgtt tccgtgggcg gcaagctcga ccggatcaat
      721 gagctgaccg atggctacac gctgccgggt gacgcttgcg ccagttacac cctgctcttc
      781 cgcctgttgc gcgaattgga ggatgacacg catgtgcaca tccacctcga gaacaacatc
      841 ctctttccca agacggcgga gctggagcgt tcgttcaatt gagcgggcaa gggggcgaag
      901 gggcgagggt ccacccagca cgctctctca ccctcgcacc ttcgcaccct cttaccctcg
      961 caccctccca tccgcgccca ctcgtacccg ggatctgacc catgaccatc acctccgaca
     1021 cgaagatcgc agccctcctg cgcgaaagcc cggacgcgct ggaggccatc atcagcctgt
     1081 cgccgaagta caacaagctg cgcaatccgt tgctgcggaa gctgatggcc tcgcgcgcca
     1141 ccatcggcat ggccagcagg atcggcgggc gcaccccgga agacttcttc cgtgtgctgg
     1201 aaccgctcgg cttcgttgtg gacaggtccg cctccaccgg tggtgaggag ccccgcacgc
     1261 ccagaccggc attcatggaa cggctctcgc cggaacaggt gcaggacctc gatgtccggc
     1321 ccatcctcaa cgcgggcaag gacccgttca acctgatcct gggcaaggtg caagcctt
//
LOCUS       DAOQOC010000985         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_81955_length_1378_cov_2.748299, whole genome shotgun sequence.
ACCESSION   DAOQOC010000985 DAOQOC010000000
VERSION     DAOQOC010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81955_length_1378_cov_2.748299"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PL106_13705"
     CDS             <1..272
                     /locus_tag="PL106_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA34177.1"
                     /translation="QKQRVAIARALVSGPKIILADEPTGALDSTTSKEVMELLKHVNR
                     DMGMTVVIVTHERAVAAASQRVIYLRDGMIENDRLDPATLLADVR"
     gene            262..>1378
                     /locus_tag="PL106_13710"
     CDS             262..>1378
                     /locus_tag="PL106_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA34178.1"
                     /translation="MFDRENWGEILQSIGKNKLRTVLTGFAVFWGIFMLILLLGLGNG
                     LRNGFAYNFRNTAENSISIWGGETSKPWNGLPANRNIQLTEEDVEALRHTIPGLEHIA
                     GNYRVWRGNSQLQYGNNYGSYNIHGIINEQQLLEKQTVAQGRFINEVDEQLKRKTIVI
                     ADDARKELFKDEDPMGKWVKVNGVPFQVVGLYASENEGRQGMMQRNPVFIPLSAAQQV
                     FNAQRDVDEIMFSFSDASLAGAAMAEQRTRHTLARMHNFDPTDDRAIWLDNNVENVST
                     FNGIFTGISGFIWFIGIGSLVAGIVGIGNIMLIVVKDRTREIGVRKAMGATPANVIGQ
                     ILMEAIFVTAVFGWFGLALGVLLLEGLASVVPGSEFFR"
BASE COUNT          300 a          422 c          426 g          230 t
ORIGIN      
        1 gccagaagca acgggtggcc atcgcgcgtg cattggtgag cggcccgaag atcatcctgg
       61 ccgacgagcc cacgggcgcg ctggacagca ccaccagcaa ggaagtgatg gaactgctca
      121 aacacgtgaa ccgcgacatg ggcatgacgg tggtgatcgt gacgcatgaa cgtgccgtgg
      181 ccgccgccag ccagcgtgtg atctacctgc gcgacggtat gatcgagaac gaccgtctgg
      241 accccgcaac cctcctggcc gatgttcgat agggagaatt ggggggagat cctccagagc
      301 atcggcaaga acaaattgcg caccgtgctc acgggcttcg ccgtgttctg gggcatcttc
      361 atgctcatcc tgctgctcgg gctgggcaat ggattgcgca atggattcgc gtacaatttc
      421 cgcaacacgg ccgagaacag catcagcatc tggggtggcg aaacgagcaa accctggaac
      481 ggcctgcccg ccaaccgcaa catccaactg accgaagagg atgtggaggc gctgcgccac
      541 accatacccg gcctggaaca tatcgctggc aattaccgcg tgtggcgcgg caacagccag
      601 ctccagtacg gcaacaacta cggcagctac aacatccacg gcatcatcaa cgagcaacag
      661 ttgctcgaga aacagaccgt ggcgcagggg cgcttcatca acgaggtgga cgaacagctc
      721 aaacgcaaga ccatcgtgat cgccgatgat gcccggaaag agctcttcaa ggacgaggac
      781 ccgatgggca agtgggtgaa ggtgaacggc gtgcccttcc aggtggtggg tctatacgcc
      841 tcggagaatg agggccgcca gggcatgatg caacgcaacc ccgtattcat acccctgagc
      901 gccgcgcaac aggtgttcaa cgcgcagcgc gatgtggacg agatcatgtt cagcttcagc
      961 gatgccagcc tggccggcgc cgccatggcc gagcagcgca cccgccacac cctggcccgc
     1021 atgcacaatt tcgaccccac cgacgacagg gccatctggc tggacaacaa cgtggagaat
     1081 gtatccacat tcaacggtat attcaccggc atctccgggt tcatctggtt catcggcatc
     1141 ggcagtctgg tggccggcat cgtgggcatc ggcaacatca tgctcatcgt ggtgaaggat
     1201 cgcacacgcg agatcggcgt gcgcaaggcc atgggcgcca cgccggccaa tgtcatcggc
     1261 cagatcctga tggaggccat tttcgtcacc gccgtgttcg gctggttcgg cctggccttg
     1321 ggcgtgctcc tgctggaagg tctggccagc gtggtgcccg gcagcgagtt cttccgca
//
LOCUS       DAOQOC010000986         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_82285_length_1375_cov_4.540909, whole genome shotgun sequence.
ACCESSION   DAOQOC010000986 DAOQOC010000000
VERSION     DAOQOC010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82285_length_1375_cov_4.540909"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1375)
                     /locus_tag="PL106_13715"
     CDS             complement(<1..>1375)
                     /locus_tag="PL106_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HNA34179.1"
                     /translation="PFFSALKEYSRKPMGVFHAMPISRGNSVFRSRWIQDLGDFYGRN
                     LFLAETSATTGGLDSLLQPTGPLKKAQELASRAFGSQRTYFATNGTSTTNKIVVQALV
                     EPGDIVLIDRDCHKSHHYGMVLSGAYPVYLHSYPLPRYSMYGAVPLEHIRENLLALKR
                     GGRLSRVKMLLLTNSTFDGVVYNTERVMEQVLAIKPDIVFLWDEAWWAFARFSYVLRQ
                     RTAMRTAEKLARKYRTPEYRAEYEAHIKSLRPGEEPRLPDPDKVRIRVYATQSTHKTL
                     TSFRQGSMIHIWDEDFTKKSETNFHEAYMIHTSTSANYQILASMDVGRRQVEFEAYEL
                     VEKAIEMGLLLRRTIRTNERLRKWFDIITIGELVPGEYRQSGLDSYYRTGSGWNEIER
                     AWTEDEFAVDPTKINLYTGKTGIDGDTFKNQYLMDKHQIQVNKTSRNSVLFMTNIGTT
                     RGSLAYLT"
BASE COUNT          221 a          393 c          443 g          318 t
ORIGIN      
        1 ggtgagatag gccagcgaac cgcgtgtggt cccgatattg gtcatgaaga gcaccgagtt
       61 gcgcgaggtc ttgttcacct ggatctggtg cttgtccatc aggtactggt tcttgaacgt
      121 gtccccgtcg atgccggtct tgccggtgta caggttgatc ttggtgggat ccactgcgaa
      181 ttcgtcctcg gtccaggccc gttcgatctc gttccagccg gagccggtgc ggtagtagct
      241 gtccagtccg ctctgccggt attcaccggg taccagttcg ccgatggtga tgatgtcgaa
      301 ccacttgcgc aaccgctcgt tggtccggat cgtccggcgc aacagcaggc ccatctcgat
      361 ggctttttca accagttcgt acgcctcgaa ctccacctgg cgccgtccca cgtccatgct
      421 ggccaggatc tggtagttcg cgctggtgct ggtatggatc atgtacgcct cgtggaagtt
      481 ggtctcgctc ttcttggtga aatcctcgtc ccagatgtgg atcatgctgc cctggcggaa
      541 cgaggtgagc gtcttatgtg tgctttgggt ggcgtacacc cggatgcgca ccttgtccgg
      601 atcgggcagg cgcggctcct ctccgggtcg cagacttttg atgtgggcct cgtactcggc
      661 ccggtattcc ggggtacggt acttgcgggc gagtttctcg gccgtgcgca tggccgtgcg
      721 ctggcgtaac acgtaactga agcgagcgaa tgcccaccag gcctcgtccc acaggaaaac
      781 gatatccggc ttgatcgcca gtacctgctc catcacacgc tcggtattgt ataccacgcc
      841 atcgaaggtg ctgttggtga gcaggagcat cttcacccgg ctcagacgac ctcctctttt
      901 gagggccagg aggttttccc ggatgtgctc cagcggtacg gcgccgtaca tgctgtaacg
      961 cggcagcgga tagctgtgca ggtacacggg ataggcgccg ctgagcacca tgccgtagtg
     1021 atggctcttg tggcagtcgc gatcgatgag tacgatgtcg ccgggctcca ccagggcctg
     1081 caccacgatc ttgttcgtgg tgctggtccc gttggtggcg aaataggtgc gttggctgcc
     1141 gaatgcgcgc gaggcgagtt cctgtgcctt cttgagcggc ccggtcggtt ggaggagcga
     1201 gtcgagcccg cccgtggttg cgctcgtctc ggccaggaag aggttgcggc cgtaaaaatc
     1261 cccaagatct tgtatccacc ggctcctgaa cacgctgttt ccacgtgaga tgggcatggc
     1321 gtggaagacg cccatgggtt tgcggctgta ctccttcagc gcggagaaga agggg
//
LOCUS       DAOQOC010000987         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_82293_length_1375_cov_3.985606, whole genome shotgun sequence.
ACCESSION   DAOQOC010000987 DAOQOC010000000
VERSION     DAOQOC010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82293_length_1375_cov_3.985606"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PL106_13720"
     CDS             complement(<1..948)
                     /locus_tag="PL106_13720"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34180.1"
                     /translation="MRTNKTRMLLPAALVLVTATAQAQNITGYRYWFNDDVASAVTVD
                     VTPTPELDAALSLNSAALPAGHHLATIQFRDADGHWGAPWTEHFTQKGGTVNAIEYWF
                     DDNAGDATTANMTPGTAPLYSTPLDATALPVGFHSVTVRTMDASGERGVPYTVPFARN
                     GGMITGYEYWLDEAIADRTTGSIGPAGVVDLVAGLPVPTTDGTHLFTIRFRDEDGGWS
                     VPLSSTFNYFVGMDEIPGLSNYLVFPNPAVDVLSIRLDAARAGTLDLSILDATGRRVG
                     GLNKWSVLGTAQHNLDISTLARGSYQLLIAEDTHQVRVPF"
     gene            complement(952..>1375)
                     /locus_tag="PL106_13725"
     CDS             complement(952..>1375)
                     /locus_tag="PL106_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34181.1"
                     /translation="RLQNASNCIVQNCVFTYTGAPLWQVSGVQISNCLVGGASMFSNS
                     GASSETNNIFNVAPGTFFVSEADDWYHYYDDIHLSPASGGVGMGNDGTDIGIFGTHAP
                     YKIGAMPYNPHFQQGAIDPATNWNGELPVQIRTAAQTH"
BASE COUNT          235 a          420 c          454 g          266 t
ORIGIN      
        1 gaaggggacc cggacctgat gcgtgtcctc cgcgatgagg agctgatagc tgccgcgtgc
       61 aagggtggag atgtccaagt tgtgctgagc tgtgcccagg acgctccatt tgttgagccc
      121 gcccaccctg cggccggtgg catccagtat gctcagatca agggtacccg cgcgcgccgc
      181 atccagccgg atgctgagca catcaaccgc cgggttgggg aacacgaggt agttgctcaa
      241 acccgggatc tcatccatgc caacgaagta gttgaaggtg ctgctcaggg gcacgctcca
      301 gccgccgtcc tcgtcgcgga aacggatggt gaacaggtgt gtgccatcgg tggtgggcac
      361 gggcaggccg gccaccaggt ccaccacacc cgccgggccg atgcttcccg tggtacgatc
      421 ggcgatcgcc tcgtctaacc aatactcata gccggtgatc atgccaccgt tgcgggcaaa
      481 gggtaccgtg tacggcacgc cgcgttcgcc cgatgcgtcc atcgtgcgta ccgtcaccga
      541 gtgaaagccc accggcaatg ccgtcgcgtc cagcggggtg gaatacagcg gtgcggtacc
      601 gggcgtcata ttggccgtgg tggcatcccc cgcgttatca tcgaaccagt actcgatggc
      661 gttcaccgtg ccgccttttt gcgtgaagtg ctccgtccag ggcgcgcccc aatggccgtc
      721 cgcatcgcgg aactggatgg tggccaggtg atggccagcg ggcagcgctg cgctgttcag
      781 cgagagtgcg gcatcgagct ccggcgtcgg ggtcacgtcc accgtgacgg ccgaggccac
      841 atcatcattg aaccaatacc gataaccggt gatgttctgg gcctgcgccg tggccgttac
      901 cagcacaagg gcggcgggca acagcatccg tgtcttgttg gtccgcatgg ctcagtgggt
      961 ttgtgcggcc gtacggatct ggacgggcag ctcgccgttc caattggtgg cgggatcgat
     1021 cgccccctgc tggaagtgcg ggttgtaggg catggcgccg atcttgtacg gcgcgtgcgt
     1081 tccgaagatg ccgatatccg tgccgtcgtt gcccatgccc actccaccgc tcgccgggct
     1141 caggtggatg tcgtcatagt aatggtacca gtcgtcggcc tcgctcacga agaaggtccc
     1201 gggtgccacg ttgaagatgt tgttcgtttc cgaactcgct ccggagttgc tgaacatgct
     1261 ggcgcctccc accaggcagt tggagatctg tacgccggac acctgccaca gcggtgcgcc
     1321 ggtgtaggtg aacacacaat tctggacgat gcagttggac gcgttctgca gtcgg
//
LOCUS       DAOQOC010000988         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_82554_length_1373_cov_3.974962, whole genome shotgun sequence.
ACCESSION   DAOQOC010000988 DAOQOC010000000
VERSION     DAOQOC010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82554_length_1373_cov_3.974962"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>1373)
                     /gene="rpoB"
                     /locus_tag="PL106_13730"
     CDS             complement(156..>1373)
                     /gene="rpoB"
                     /locus_tag="PL106_13730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791264.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNA34182.1"
                     /translation="GEKAGDVKDASMKAPPSTKGFVIDKKLFSRAIKDKKSKGDEKDV
                     LERIDREEEKELADLKDTLINKMMTLLEGQTSAGVDNKYKEEQVKKGVKFSAKVLQAV
                     DYITVDPTEWTTDKRTNELVRQLIHNYSIRYNDISGAYKRKKFQVSIGDELPAGIVKL
                     AKVYIAAKRKLTVGDKMAGRHGNKGIVAKIVRDADMPYLDDGTPVDIVLNPLGVPSRM
                     NLGQIYETVLGWAGLRLGRKYATPIFDGATIDEINTETDEAGIPRFGKAYLTDGGTGE
                     RFDQPATVGVIYMLKLAHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAFGAANILQEILTVKSDDVMGRAKAYEAIVKGEPLPTAGIPESFNVLLHELRGLAL
                     NVSLESETEKIQL"
BASE COUNT          195 a          418 c          423 g          337 t
ORIGIN      
        1 cgggcttgta ggtgcggtag ttgatcgttt cgggcttgat cacctccccg tggctccgct
       61 ccaggatctg ctccgggctg gccaggctga tgacgatctt atcgaactgc gaggtgagct
      121 tcacctcctt cttcatgatg gtcatggtgt atggctcaga gttggatttt ctcggtttcg
      181 gactccaggc tcacgttcag cgcgaggccg cgcagttcgt ggagcagcac gttgaatgac
      241 tcggggatgc cggccgtggg cagcggctca cctttcacga tggcctcgta cgccttagca
      301 cggcccatca cgtcgtcgct cttcacggtg aggatctctt gcaggatatt ggcggcaccg
      361 aaggcctcca gcgcccacac ttccatttca ccgaagcgct gaccaccgaa ctgggcctta
      421 ccgcccaggg gttgctgcgt gatgaggctg taggggccga tggagcgcgc gtgcatcttg
      481 tcgtccacca tgtgggcgag cttcagcatg tagatcacgc ccacggtggc gggctgatcg
      541 aaacgctcgc ccgtaccacc gtcggtgagg taggccttgc cgaatcgggg gatgccggcc
      601 tcatcggtct cggtgttgat ctcgtcgatg gtggcaccgt cgaagatcgg ggtggcgtac
      661 ttgcggccga gtctgaggcc ggcccatccg agcacggtct cgtagatctg gccaaggttc
      721 atgcggctcg gcacgcccag ggggttcagt acgatgtcca ccggtgtacc gtcatccagg
      781 tatggcatgt cggcatcgcg cacgatcttg gccacgatgc ccttgttgcc gtggcgaccg
      841 gccatcttgt cgcccacggt gagcttgcgc ttcgcggcga tgtaaacctt ggccagcttc
      901 acgataccgg cgggcagttc gtcgccgatg ctcacctgga attttttgcg cttgtaggcg
      961 ccgctgatgt cgttgtagcg gatggagtag ttgtggatca actggcgtac cagttcattg
     1021 gtacgcttgt ccgtggtcca ctccgtgggg tccacggtga tgtagtcaac ggcctggagc
     1081 accttggcgc tgaacttcac gcccttcttc acctgctcct ccttgtactt gttgtccacg
     1141 cccgcgctgg tctgcccctc cagcagggtc atcatcttgt tgatgagggt gtccttcagg
     1201 tcggcgagtt ccttctcctc ctcgcggtcg atgcgctcga gcacgtcctt ctcatcaccc
     1261 ttgctcttct tgtctttgat cgcccggctg aagagtttct tgtcgatcac gaagcccttg
     1321 gtgctggggg gcgccttcat gctggcgtcc ttcacgtcgc cggccttctc gcc
//
LOCUS       DAOQOC010000989         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_82606_length_1373_cov_2.657056, whole genome shotgun sequence.
ACCESSION   DAOQOC010000989 DAOQOC010000000
VERSION     DAOQOC010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82606_length_1373_cov_2.657056"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PL106_13735"
     CDS             complement(<1..259)
                     /locus_tag="PL106_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34183.1"
                     /translation="MTQKATIRQRWRRFIYFFPFQLFVLHLKKNPLLLLLWLVLFGYI
                     AQVMGVKYGVPYLFLVPEYFGRTSFWSFGILGFAAGGFFTAF"
     gene            complement(318..>1373)
                     /locus_tag="PL106_13740"
     CDS             complement(318..>1373)
                     /locus_tag="PL106_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNA34184.1"
                     /translation="AIAFDIREAGRKVKQADGSEKQVPGKLKTVKGIGWFIEEYGVAQ
                     LSLNLTDLDVTPMHIAFDEACKSAQERGIRVTGSELVGLVPKQALLDAADFFLRRQQR
                     SLGLPEREKIKIAVKSLGLDDLAPFDPQKRVIEYQLANAADSRLAHMDLERFSEETAR
                     ETPAPGGGSVAAYVGALGAALGTMVANLSAHKRGWDERWEEFSTWAEKGEAFRRDLLR
                     LVDEDTRAYDGILAAMQLPKENEAQKAARKDAIREATKGAILVPLRTLELCVESMSVM
                     KAMCEKGLKASVSDAGVGALCARSGAIAAYLNVRINCVGLDDEAWKKDVLAKADRLKA
                     EAERMEAEVMAITLAKT"
BASE COUNT          228 a          469 c          402 g          274 t
ORIGIN      
        1 tgaaggccgt gaagaagccg cctgccgcga atcccaggat gccgaaactc cagaaactgg
       61 tacgaccgaa atactcaggg acgaggaaga gatagggcac gccgtatttg acgcccatca
      121 cctgcgcgat gtatccgaag agcaccagcc acagcaacaa cagcagtggg ttcttcttca
      181 ggtgaagcac gaacaattgg aaggggaaga agtagatgaa ccttctccac cgctgccgga
      241 tcgtggcctt ctgcgtcatc gcgcacaagg tatacgtgcg agggcctgcc ggtgttgcct
      301 ctctacactt accgggatca ggtcttggcc agggtgatgg ccatcacctc ggcctccatc
      361 cgttccgctt cggccttcag ccggtcggcc ttggccagta cgtccttctt ccaggcctca
      421 tcatccagcc ccacgcagtt gatccgcacg ttcaggtagg ccgcgatggc tccggaccgt
      481 gcgcacaggg cacccacacc cgcatcgctc acgctggcct tcaacccctt ctcgcacatg
      541 gctttcatca ccgacatgct ctccacgcac agttccagcg tgcgcaacgg caccagaatg
      601 gcacccttgg tggcctcgcg gatggcgtcc ttgcgcgcgg ctttctgcgc ttcgttctcc
      661 ttgggcagtt gcatggccgc caggatgccg tcgtaggcgc gtgtgtcctc gtccaccagg
      721 cgcaacaggt cgcgacggaa cgcctcgccc ttttcggccc aggtgctgaa ttcctcccag
      781 cgctcgtccc agccacgctt gtgggcactg aggttggcca ccatggtgcc caatgcggca
      841 ccgagcgcgc ctacgtacgc cgctacgctt ccgccaccgg gggcgggggt ttcccgggcg
      901 gtctcctcgc tgaagcgctc caggtccatg tgcgccagtc ggctgtccgc tgcgttggcc
      961 agctggtact cgatcacgcg cttctgcggg tcgaaaggag cgaggtcgtc cagtccgagg
     1021 gacttcaccg cgatcttgat cttctcccgt tcgggcaggc ccaggctgcg ctgctgccgc
     1081 cgcaggaaga agtcggccgc gtccaacaag gcctgtttgg gcaccaggcc caccagttcg
     1141 ctaccggtaa cgcggatgcc gcgctcctgc gcgctcttgc acgcctcgtc gaaggcgatg
     1201 tgcatcggtg tcacgtcgag atcggtcagg ttgagcgaga gctgcgccac cccgtactcc
     1261 tcgatgaacc acccgatgcc cttcaccgtt ttcagcttgc ccggcacttg tttctcgctg
     1321 ccatcggcct gcttcacttt tcggcctgct tcgcggatgt cgaaggcgat ggc
//
LOCUS       DAOQOC010000990         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_82833_length_1371_cov_3.066869, whole genome shotgun sequence.
ACCESSION   DAOQOC010000990 DAOQOC010000000
VERSION     DAOQOC010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82833_length_1371_cov_3.066869"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>1371)
                     /locus_tag="PL106_13745"
     CDS             complement(31..>1371)
                     /locus_tag="PL106_13745"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNA34185.1"
                     /translation="VDGSYQAWWTPSTYGPFSVTMSAAANTGNVTDTTVSVTVSNTIA
                     DQTVATFDGDVIDMGTIGSQWFYGTYTLPQSVGAYEQIIAHFDVTCPSVPGGCDDWDR
                     LAWIQFKAPNGQWMELIRYITPYGRACSHTVDVTDFASLLQGNIEVRMFIDTYGTGGW
                     KLDLDLEYKAGTPTFPYTTVQEVWQGSFPFGDPANLQPMDTVSLTPSWNASACALRLV
                     TTGHGWGDNNSGNAAEFYHAQHELQVDDAFAYQQDLWTNCSPNPDGCQPQSGTWQYDR
                     AGWCPGTIPQPGHYDLTAYMSAAPFDLAYIFQPSYQDLCHPNNPDCISGTTCPDCGAG
                     YNPFYRVSGYFISKGTQPISIGMAEQNANDAGTLWVYPNPADDRFQVVATGMEGPGRV
                     RVIAATGAEVLQWRFNHANTLAARSFSSEALNAGVYTVVVESTSARQVGRLVVR"
BASE COUNT          223 a          408 c          459 g          281 t
ORIGIN      
        1 ccatgagtgc tcgttccctg gtatcacacc tcaccgcacc accaaccggc ccacttgccg
       61 tgcgctggtg ctttccacca ccacggtgta cacaccggcg ttgagcgctt cactgctgaa
      121 gctgcgcgcg gccaatgtat tcgcatggtt gaagcgccac tgcagcactt cggcacccgt
      181 ggcggcgatc acgcgcacgc ggcccggccc ctccatgccg gtggccacca cctggaagcg
      241 gtcgtcagca gggttcggat acacccacaa ggtgcccgca tcgttggcgt tctgttccgc
      301 catgccgatg ctgatcggtt gtgtaccctt ggagatgaaa tatccgctga cgcgatagaa
      361 cgggttgtag cccgcgccgc aatcggggca ggtggtaccg ctgatgcaat ccgggttgtt
      421 cgggtggcag aggtcctggt acgaaggctg gaagatgtac gccagatcga acggcgcggc
      481 ggacatgtat gcggtgaggt cgtagtggcc tggctgcggg atggttccgg ggcaccagcc
      541 ggcgcggtcg tactgccagg ttccgctctg cggctggcaa ccgtccggat tcgggctgca
      601 gttggtccac aggtcctgct ggtaggcgaa ggcatcgtcc acctgcagtt catgctgtgc
      661 gtggtagaat tccgcagcat tgccgctgtt gttgtcgccc cagccgtgcc ccgtggtgac
      721 gagccgcaat gcgcacgcgc tggcgttcca cgagggtgtg agcgacaccg tgtccatggg
      781 ttgcaggttg gcggggtcgc cgaaggggaa ggagccctgc cacacttcct gcaccgtggt
      841 gtaggggaag gtgggtgtgc cggccttgta ttccaggtct agatcgagtt tccaaccgcc
      901 ggtgccgtag gtgtcgatga acatgcgtac ttcgatgttg ccttgcagta acgaggcgaa
      961 gtcggtgaca tccacggtgt gcgagcatgc gcggccgtac ggcgtgatgt agcggatcag
     1021 ttccatccat tggccgttgg gcgccttgaa ctggatccac gccaagcggt cccaatcgtc
     1081 gcagccgccg ggtaccgagg ggcaggtcac atcgaagtgt gcgatgatct gctcatatgc
     1141 acccaccgat tgcggcagcg tgtaggtgcc gtagaaccat tgcgagccga tggtgcccat
     1201 gtcgatcacg tccccatcga aggtggccac cgtctggtcg gcgatggtgt tgctcacggt
     1261 gacgctcacc gtggtgtcgg tcacgttgcc ggtgttggct gcggcgctca tcgttacgct
     1321 gaaaggaccg taggtgctgg gcgtccacca ggcctggtac gatccgtcca c
//
LOCUS       DAOQOC010000991         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_82916_length_1370_cov_4.157414, whole genome shotgun sequence.
ACCESSION   DAOQOC010000991 DAOQOC010000000
VERSION     DAOQOC010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82916_length_1370_cov_4.157414"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1195
                     /locus_tag="PL106_13750"
     CDS             17..1195
                     /locus_tag="PL106_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNA34186.1"
                     /translation="MALSPQEQALAVGTRGRNLFIGIPKETSAQERRVPLTPASVAVL
                     VGRGNDVTVERGVGNAVQFQDSDYTEAGAVLAETAEEVFKGDIILKVAPPSPAEIELM
                     RPKQLLISALQMTTQPKDTLRRMMEKKVTAVAWDFIRDREGIYPIIRAMGEIAGNASI
                     QIAAEYLSTDKGGPGLMLGGISGVAPAEVVVIGAGTVGEFAVRAASGLGASVKVFDKS
                     IYRLRRLQTDLDQRIWTSVIQPDELRRALQQADVAIGALRPEHGRTPMVVNEDMVREM
                     KNGSVIVDVSIDRGGCFETSRPTDLSDPVFKKFGVLHYCVPNIASRVPRTASYALTNI
                     FAPLLLGMGDVGGFEELIKRDLGLRHGVYLYNGALTSEVLSEAFRLPYKDLDILLAAF
                     "
BASE COUNT          270 a          387 c          464 g          249 t
ORIGIN      
        1 acaactcgcg cgtgaagtgg ccctgtcccc gcaggaacag gcgctcgccg tgggcacgcg
       61 tggaaggaac ctgttcatcg gcatccccaa ggagacctcg gcgcaggaac gtcgtgtgcc
      121 actgacgccc gcgagtgtgg ccgtgctggt gggcaggggc aatgacgtga cggtggagcg
      181 tggcgtgggc aatgcggttc aattccagga cagtgattac acggaggcgg gtgcggtact
      241 ggctgagacc gccgaagagg tcttcaaggg ggacatcata ttgaaagtgg caccgccttc
      301 accggcggag atcgagctga tgcgccccaa gcaactcctg atcagtgcgc tgcagatgac
      361 cacgcagccc aaggataccc tgcgacggat gatggagaag aaggtgaccg ccgtggcatg
      421 ggatttcatc agggaccgtg agggcatcta tccgatcatc cgtgccatgg gcgagatcgc
      481 gggcaacgcg agcatccaga tcgcggcgga gtatctcagc accgataaag gagggcccgg
      541 cctgatgctc ggcggtatca gtggcgtggc tcccgccgag gtggtggtga tcggtgcggg
      601 cacggtgggc gagttcgccg tgcgggcggc ctcgggcctt ggtgccagcg tgaaggtgtt
      661 cgacaagagc atctatcggt tgcgacgact gcagaccgat ctggaccagc gcatctggac
      721 cagtgtgatc cagcccgatg aattgcgaag ggccctgcaa caggccgatg tggccatcgg
      781 ggcgttgaga cccgaacatg gccgcacccc catggtggtg aacgaggaca tggtgcgcga
      841 gatgaagaac ggcagcgtga tcgttgatgt cagcatcgat cgcggcggtt gtttcgaaac
      901 gagccgcccg accgacctca gcgatccggt cttcaagaaa ttcggggtcc tgcactattg
      961 tgtgcccaac atcgccagtc gggttccacg taccgccagt tacgcactca ccaacatctt
     1021 cgctccattg ctcctgggca tgggcgatgt gggcggtttc gaggaactca tcaaacgtga
     1081 cctgggcctg cgccatggcg tgtacctgta caatggggcc ctgacgagtg aggtgttgag
     1141 cgaggcgttc cggcttccgt acaaggacct ggacattctt ctggccgcat tctgacgggg
     1201 catcgacatg acattcggaa gacgcttgcg cctgttcatg ctcggactcg gactggggac
     1261 catcgtttcg ttcatgatct tcgggaagag ctgtaccaac acggcatggg cgcctgaggc
     1321 ccgggtgaag ctgcggatga agagcaccct cacccgagcg acccccgcag
//
LOCUS       DAOQOC010000992         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_83144_length_1368_cov_4.209444, whole genome shotgun sequence.
ACCESSION   DAOQOC010000992 DAOQOC010000000
VERSION     DAOQOC010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83144_length_1368_cov_4.209444"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PL106_13755"
     CDS             <1..328
                     /locus_tag="PL106_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNA34187.1"
                     /translation="QAAEQLAERGIRAEVINVHTIKPLDEAAVLASVRKTGCAVTCEE
                     HNRYGGLGDTIAQVLVRHAHVPQEYVAVNDSFGESGTPDQLLKKYGLDTPDIVKAVER
                     VMARKA"
     gene            429..980
                     /locus_tag="PL106_13760"
     CDS             429..980
                     /locus_tag="PL106_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA34188.1"
                     /translation="MSADLSDEELLALFAQEDRRHYAFNLIVRKHQQRLYSFVRRMVT
                     DPDETQDVLQNTFIKAWNGLGNFRGDAQLYSWLYRIAHNESISHLARMRRGLFTSDER
                     VFDRLTTTLDNSEHFSGDAIQLKLQKAIMRLPAKQRAVFNMRYFDEMKYEDMSQVTGT
                     SVGALKSSYHIAVKKIEEWVTGE"
     gene            1027..>1368
                     /locus_tag="PL106_13765"
     CDS             1027..>1368
                     /locus_tag="PL106_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34189.1"
                     /translation="MERSDEMDELKHLAPTLHGLKRTDPFTVPDGFFERFPHQVQAAI
                     VERQRSSAPAWTWWMRAAIALPVVALAVTGLWMLRTQQPDATAQPVAVTPLTDRELDA
                     LDDAEVLAAFDL"
BASE COUNT          295 a          427 c          416 g          230 t
ORIGIN      
        1 gcaagcagcg gagcagctgg ccgagcgcgg catccgggcc gaggtgatca atgtacacac
       61 gatcaaacct ctcgatgaag cggccgtctt agcctctgtc cggaaaacgg gatgcgcggt
      121 cacctgcgaa gaacacaatc ggtacggcgg cctgggcgat accatcgctc aggtactggt
      181 gcgccatgcc catgttccgc aggagtacgt ggccgtgaac gacagcttcg gcgagagcgg
      241 aacaccggac cagttgctga agaagtacgg actggacacg cccgatatcg tgaaagctgt
      301 ggagcgcgtg atggcgcgga aggcctgaag ttgaattctg agtggcgagt ggtgagtggc
      361 gagtggccgc tgtggataca gttctgccac tcgcctctcg cctctcgcct ctcgccacct
      421 acggtccgat gagcgcagat ctttccgacg aggaactctt ggccctgttc gctcaggagg
      481 atcgccgaca ctacgccttc aacctgatcg tgcgcaagca ccagcagcgg ctgtacagtt
      541 tcgtgcggcg catggtgacc gatccggatg aaacgcagga cgtgctccag aacaccttca
      601 tcaaagcatg gaacggcctg ggcaacttcc gcggcgatgc ccaattgtac agttggctct
      661 accgtatcgc ccacaacgag agcatcagtc acctggcccg catgcggcgt gggctgttca
      721 ccagcgatga acgggtcttc gatcggctca ccaccaccct ggacaacagc gagcacttca
      781 gcggcgacgc catccagctc aaactgcaaa aggccatcat gcgcctgccg gccaagcagc
      841 gcgcggtatt caacatgcgg tacttcgacg agatgaaata cgaggacatg agccaggtga
      901 ccggcaccag cgtgggcgcg ctgaagagca gctaccacat cgccgtgaag aagatcgagg
      961 agtgggtcac cggagaatga tggatcaaac cttcgcgcac acgaacggtc aatagagcaa
     1021 gaagccatgg aacggtccga cgaaatggac gaactgaagc acctcgcgcc cacgttgcac
     1081 gggctgaaac gaacggaccc gttcaccgtg ccggacggtt tcttcgagcg ctttccgcac
     1141 caggtacaag cggccatcgt cgagcgccag cgttcgtccg ctcccgcgtg gacgtggtgg
     1201 atgcgcgcgg ccatcgccct gccggtggtc gccttggccg ttaccggtct ttggatgctt
     1261 cgcacgcaac aacccgacgc aaccgctcaa cccgtagcgg tgacacccct gaccgaccgc
     1321 gaactcgacg cgctggacga tgcggaggtc ctcgctgcct tcgacctc
//
LOCUS       DAOQOC010000993         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_83427_length_1366_cov_2.726926, whole genome shotgun sequence.
ACCESSION   DAOQOC010000993 DAOQOC010000000
VERSION     DAOQOC010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83427_length_1366_cov_2.726926"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..746
                     /locus_tag="PL106_13770"
     CDS             192..746
                     /locus_tag="PL106_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34190.1"
                     /translation="MNYEECFKRAMGGNEDTPYNYQRELACREQGDSAEDQGLTSAAA
                     CRSQLIDIPTGLGKTAGVVLAWLWNRVVLQRPDWPRRLVYCLPMRTLVEQTGSEVKGW
                     IGNVLKHSGDLAERTVAGLKWLSDPSPIVLKGATLDPSFMHLGEGPNGPSWLARTTAQ
                     RDHLGPFSLACLLRAADARASRTA"
     gene            750..>1366
                     /locus_tag="PL106_13775"
     CDS             750..>1366
                     /locus_tag="PL106_13775"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNA34191.1"
                     /translation="MDYKTLLDGIAQAHQHTLAGVARTVNRALVVRNWLIGAHLVEFE
                     QHGDDRAAYGTGLLKRVAKDLANAGVPGCGVRMLERMRTCYLTYPQVASAIPSPLVTK
                     SLKLLDASASRNSSPPVTISGATSERLPTPLGHELVLGLSWTHLIDLLGIDDPWKRAF
                     YENECIKGGWSKRQLQRQIGSLLYERTGLSTDKQAVIERARQQAM"
BASE COUNT          267 a          424 c          426 g          249 t
ORIGIN      
        1 gttggggcta cttcgccgtc atgggcttca tggccgtgct ggccgtgggg atgctcgtgt
       61 ggttcaggag aaagaggtgg ttgtaggggg tcggccaagc tcgcgcgttt cccacagcct
      121 ttgaacactg ggtccgaacc gctactttgt gaaccgctga tcgccagaaa taccggcgat
      181 cggaacggac gatgaactac gaggaatgct tcaagcgggc gatgggcggc aatgaggaca
      241 cgccatacaa ctaccagcgc gaactggcct gcagggaaca gggcgacagc gctgaggacc
      301 aagggctgac cagcgccgct gcgtgccgct cccaactcat cgacatcccc accggcctgg
      361 gcaagaccgc tggcgtggtg ctggcttggc tgtggaaccg cgtggtgctg caacgtcccg
      421 attggcccag aaggcttgtg tactgcctgc cgatgcggac gttggtggag cagacgggaa
      481 gcgaagtgaa ggggtggatc ggtaatgtgc tgaagcattc cggtgatctt gctgaacgaa
      541 ctgttgccgg tctcaagtgg ttgagcgatc cctccccaat tgtgctcaaa ggagccaccc
      601 tcgacccttc cttcatgcac ctgggcgaag ggccgaacgg cccctcatgg cttgcccgca
      661 ccacggcgca gcgcgaccac ttgggaccct tcagccttgc ttgtctcctc cgcgccgccg
      721 acgcacgagc ttcacgaacg gcctgaagca tggattacaa gacgctcctc gacggcatcg
      781 cacaagcgca ccagcacacc cttgcaggtg ttgcccgcac cgtgaaccgg gcactggtgg
      841 tgcgcaactg gctcatcggc gcacatctgg tggagttcga gcagcacggc gacgaccgcg
      901 ccgcatacgg taccgggctg ctcaagcgcg tggccaagga cctggctaac gccggtgtgc
      961 ccggttgcgg ggtgcgcatg ctggaacgca tgcgcacctg ctatctcacc tatccccagg
     1021 tagcttcggc gattccgtca ccactggtga cgaaatcgct gaaactactg gatgccagcg
     1081 cctcccggaa ttcgtcacca ccggtgacga tttccggggc cacatccgaa cgcctgccaa
     1141 caccgctcgg ccatgaactg gtcttgggcc tttcatggac gcacctgatc gacctgctcg
     1201 gcatcgatga cccttggaag cgcgccttct atgagaacga gtgcatcaag ggcggatggt
     1261 ccaagcgcca gttgcaacgt cagatcggtt cgctgcttta tgaacgcacc ggactttcca
     1321 ccgacaagca ggcggtgatc gaacgcgcac gacagcaggc catgga
//
LOCUS       DAOQOC010000994         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_83507_length_1365_cov_4.193893, whole genome shotgun sequence.
ACCESSION   DAOQOC010000994 DAOQOC010000000
VERSION     DAOQOC010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83507_length_1365_cov_4.193893"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PL106_13780"
     CDS             <1..674
                     /locus_tag="PL106_13780"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNA34192.1"
                     /translation="AASLLSALHTGADGTEMDVRLTLDGKLVAFHDLDLSVSTTGTGA
                     IADLTWAQVAQARYLQPLYGDHGIVDIDHFLSHADVPSGAILYFDVKPDPGAQDPALY
                     RERMADAMAALFTRHDLLARSFVECRDIGIAVAVQARLPSARLLSYSPSFDEALGLAV
                     QHDLFGVSHFGDQVSADQVRTAHEQGKWVALVAERRNEQRSTGEKGPEIIISDELKHL
                     ADLLE"
     gene            complement(685..1191)
                     /locus_tag="PL106_13785"
     CDS             complement(685..1191)
                     /locus_tag="PL106_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNA34193.1"
                     /translation="MNLAALINAARASVVKRWLLNAMLDRMIPFNRPHGFRVEPMLKG
                     GIKVHVPYRRINRNHIKGIHACALATAAEYCCGLALLEHLDPSRFRLIMKTLHMEYHY
                     QARTRAFAVFDPGATAIAEQVSGPLRTQEAVLYSGLVELHDVKNNHLATATITWQVKA
                     WDKVRTKG"
BASE COUNT          242 a          437 c          439 g          247 t
ORIGIN      
        1 ccgcagccag cctgctgtcc gcgctgcaca cgggcgccga cggcaccgag atggacgtgc
       61 ggctcacgtt ggacggcaag ctcgtggcct tccacgacct ggacctgtcc gtgagcacaa
      121 caggtaccgg ggcgatcgcc gacctcacgt gggcccaggt ggcccaggca cgttacctgc
      181 aaccgctgta cggtgaccat ggcatcgtgg acatcgacca tttcctgtcg catgccgatg
      241 taccgtccgg ggcgatcctc tatttcgatg tgaaaccgga ccccggtgcc caggacccgg
      301 ccctgtaccg cgagcgcatg gccgacgcca tggccgccct gttcacccgg cacgacctcc
      361 tcgcgcgcag cttcgttgaa tgccgggata tcggtatcgc cgtcgctgtt caggcacgcc
      421 tgccctccgc acggctcctg tcctattcac catcgttcga cgaggctctc gggctggccg
      481 tgcagcatga tctgttcggc gtatcgcatt tcggcgacca ggtgagcgcc gaccaggtgc
      541 gcacggcgca tgaacagggc aagtgggtgg ccttggtggc cgagcgccgc aacgagcaga
      601 ggagtacggg cgagaaaggt ccggagatca tcatcagtga tgagctgaag cacttggcgg
      661 acctgctgga gtgacggcac ccgctcagcc tttcgtgcgc accttgtccc aggccttcac
      721 ctgccaggtg atcgtggccg tggccaggtg gttgttcttc acatcatgca attccaccag
      781 accggaataa agaacggcct cctgcgtgcg cagcggtccg ctgacctgtt cggcgatggc
      841 ggtggccccg gggtcgaaca ccgcgaaagc gcgggtgcgg gcctggtaat ggtactccat
      901 gtgcagggtc ttcatgatca gccggaacct cgaggggtcc aggtgttcca gcagggccag
      961 cccgcagcaa tactcggccg cggtggccag ggcacaggcg tggatcccct tgatgtggtt
     1021 gcgattgatc cgccggtacg gaacatgcac cttgatgcca cccttcagca tcggctccac
     1081 ccggaaccca tgcggcctgt tgaaggggat catccggtcg agcatggcat tgagcagcca
     1141 gcgtttcacc accgaggccc gggcggcatt gatcagggcc gcgaggttca tggagtgatg
     1201 cgcgagtgga acatgggatc acatccggta cgggaccggg catgggctca attgcatagc
     1261 aggcctattg atcaacacgg ggacaagcat cctgcgatgc ggccagcacg cgtgcgatat
     1321 cgggccggaa gtaccgatcg ctcttgagca ccttgccatc ctccc
//
LOCUS       DAOQOC010000995         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_83518_length_1365_cov_3.787023, whole genome shotgun sequence.
ACCESSION   DAOQOC010000995 DAOQOC010000000
VERSION     DAOQOC010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83518_length_1365_cov_3.787023"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..446)
                     /locus_tag="PL106_13790"
     CDS             complement(186..446)
                     /locus_tag="PL106_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNA34194.1"
                     /translation="MKSGIHPESYRIVVFKDMSNEHMFLGRSCANTKDTIKWEDGNEY
                     PLVKLDISNTSHPYYTGKMMLIDTAGRVDKFNKRHARHTAAK"
     gene            942..>1365
                     /locus_tag="PL106_13795"
     CDS             942..>1365
                     /locus_tag="PL106_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34195.1"
                     /translation="MHIRTVLLTASIVILATACRKDEVFTDDPGAQLTLTVDTVLFDT
                     IFTTVGSVTKRFTVHNDNDNAVRVDIALEGGTPSPFRINVDGASGVTFNDVEILGHDS
                     IFIFVEATLGENNANDPFIFEDHILLNTNGNEQSVLLLA"
BASE COUNT          291 a          407 c          354 g          313 t
ORIGIN      
        1 cgtcccggta actctggaac agttccttcg agcagagcag gcacgtgcct atcatagcga
       61 cccggtcctt ctatgttctc aagcgatctg atgcataacc gctgttaagc tccgaccttc
      121 cggaaatgac gaagcccttc accacctgac ggcgaggaag ggcttcggta aggtgcggac
      181 gcgatctact tggccgcggt atggcgcgcg tggcgcttgt tgaatttgtc cactcgacca
      241 gcggtgtcga tcagcatcat cttgccggtg tagtacgggt ggctggtatt gctgatgtcc
      301 agcttcacca aggggtactc attgccgtcc tcccacttga tggtgtcctt ggtgttcgcg
      361 caactcctgc caaggaacat gtgctcgttg ctcatgtcct tgaaaacaac gatgcggtag
      421 ctctcgggat ggatgcctga tttcatgatc gttttcgctt ggtcggatgg ccggttcttg
      481 aggttctcct tcgccgagcg tacggcgaac gggccgcgaa tgtaatgcgt tcgggggaag
      541 aatggttcac gagttccata tcgggacctg acggagaagt tgcttggtca agcacgtacc
      601 attctaaata agcccgccca ttacatggct ggccaagcaa gtattgatga cagcgtagcc
      661 acagcgctat tttcctcgtt ttcaggggat acctggcact atctccccga aacttcagac
      721 gaaaattata tagtctgagc acaaaatgct tgtttggtca gccaagcagg attctgaata
      781 agcggctcca atggggtccc atagtcgacc tcacaacccc tgaaacgaac aacgcatttg
      841 ccccttggcc gaacgctcca ttaccatcgc cctgcgggac catgcaatga tcgcctgcct
      901 caacacgtac tccgcctatc catctttctt tgcggccctg catgcacatc cggacagttc
      961 tcctcaccgc ctcgatcgtg atcctggcaa ccgcttgccg caaggacgaa gtgttcaccg
     1021 atgaccccgg ggcccagctc acgctcaccg tggacaccgt cctgttcgat accatcttca
     1081 ccacggtcgg atcggtgacc aaacgcttca ccgtacataa cgacaacgac aacgcggtac
     1141 gggtggacat cgccttggaa ggaggcaccc cctccccttt ccggatcaat gtggacggcg
     1201 cttcaggcgt cacattcaac gacgtggaga tccttgggca cgacagcata ttcattttcg
     1261 tggaggccac cctcggcgag aacaacgcga acgacccctt catcttcgag gaccacatcc
     1321 tgctgaacac gaacggcaat gagcagagcg tgttgttgct cgcct
//
LOCUS       DAOQOC010000996         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_83622_length_1364_cov_5.943468, whole genome shotgun sequence.
ACCESSION   DAOQOC010000996 DAOQOC010000000
VERSION     DAOQOC010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83622_length_1364_cov_5.943468"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..>1364)
                     /locus_tag="PL106_13800"
     CDS             complement(414..>1364)
                     /locus_tag="PL106_13800"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34196.1"
                     /translation="SGDDVQRFLGAWFLLGVWTGVLEEFLFGRRFRALAIPLQFLGKV
                     LAVNMLTLVFIIIAFLLGSEQDLPLAQDAPFSPKQMMLMPQLYRLMLRVVVVSSIAIL
                     IVQVEEFMGRRMFLGFLLGRFERPVAEERIMLSIDLVGSTALNERLGDLLYFRFLNTV
                     HSLMTDAVLRNEAEIHKYIGDEVIFTWTMRKGVDHHNCLDLYFDIMARIAANESMLMQ
                     EFGVVPKFRGALHGGRVITAQTGHIKRTIDFSGDVMNTLNRMLTVAKDLKADLVVSAE
                     LLARMPGATARFAFGEERTMPVKGRKREVRVHVVERVKAV"
BASE COUNT          289 a          404 c          432 g          239 t
ORIGIN      
        1 aggctcagcg tgacagagca tgggcgcttc ggcaggctca gcgtgacaag tggagagtag
       61 ggaagcgatt cggcaggctc agcgtgacag agcatgggcg cttcggcagg ctcagcgtga
      121 caagtggagg agaataggga atcgcttcgg tccttccttc gtcaggacag gcgcactcag
      181 tgtgacacgg gtggggagct tgcatggcct cgcgggatcg tactgaactg tcacgcggag
      241 cgtggctacg gaacgatcgg gtctgagaat tcatacgaaa tatgcttcat cccacttgcg
      301 agaagggcaa gcaggacctg tactgccaag gcggtatcgg accaggctta tttgcgagat
      361 ggtattccct gcggttccgt gcccgagcag gaccaaccag tgccccaaaa ccttcacacg
      421 gccttcacgc gctccaccac gtgcacgcgc acctcgcgct tgcggccctt cacgggcatg
      481 gtgcgttcct ccccgaaagc gaaccgtgcg gtggccccgg gcatgcgggc caggagttcg
      541 gcactcacca ccagatcggc cttgaggtcc ttggccacgg tgagcatgcg gttgagggtg
      601 ttcatcacat cgccgctgaa atcgatggtg cgcttgatgt ggccggtctg ggcggtgatc
      661 acgcggccgc cgtgcagcgc gccgcggaac ttgggcacca ccccgaactc ctgcatcagc
      721 atgctttcgt tagcggcgat acgcgccatg atgtcgaagt agaggtccag gcagttgtgg
      781 tgatcgaccc ccttgcgcat ggtccaagtg aagatcacct cgtcgccgat gtacttgtgg
      841 atctcggcct cgttgcgcag aacggcatcg gtcatgagcg aatggaccgt gttcaggaaa
      901 cggaagtaca gcaggtcgcc gagccgttcg ttcagggcgg tgctgcccac cagatcgatg
      961 ctgagcatga tgcgctcctc ggccacgggc cgttcgaaac gaccgagcag gaaaccgagg
     1021 aacatgcggc ggcccatgaa ctcctccacc tgcacgatca agatggcaat ggacgacacc
     1081 accaccacgc gcagcatcag ccggtagagc tggggcatca gcatcatctg tttcggactg
     1141 aatggagcgt cctgagcgag gggaagatcc tgttcgctcc cgagcaagaa tgcgatgatg
     1201 ataaaaacca gggtgagcat attcaccgcg aggaccttgc ccaggaactg caggggaatg
     1261 gccagggcac ggaagcgtcg gccgaatagg aactcctcca gcacgccggt ccacaccccc
     1321 agcaggaacc acgcgcccaa gaaccgttgc acatcgtcgc cact
//
LOCUS       DAOQOC010000997         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_83678_length_1364_cov_2.802903, whole genome shotgun sequence.
ACCESSION   DAOQOC010000997 DAOQOC010000000
VERSION     DAOQOC010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83678_length_1364_cov_2.802903"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1364)
                     /locus_tag="PL106_13805"
     CDS             complement(<1..>1364)
                     /locus_tag="PL106_13805"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA34197.1"
                     /translation="GYQTYINQFSGSPFIAEAIIARDELAFQEAHAQNTADAYRRFLD
                     MHPDAAQVELATAHMQEATFREAVPNGTLVEYEGFIADHPGSPMVADAYRGIYRSVAP
                     ERTPEQLYAFIRRYPKNPYVDEAWRAICEDRTKDLSAASITAFLKDYPDYPFVQELSA
                     LYETAGLVLHPFRRNGHWGYIDTLGVERIKAVYDFAEPFHGQQAQVGRDGSVGTVSKA
                     GKEVVPVEYEDVLAFSEGLATVERDGRAGVVDRSGRTIVGLAYDEIGEYSGGLAFAAK
                     DGEFGYLDPDGDVAIPFTFDDAQTFHDGFAVVARNDVFGAIDVSGRIVVPFEYEWIER
                     FGAPLVRARRNGKMGLINEKGEEKLVAEHGYVGPFSQGLALVVDKGRCGYVDTTGAFV
                     IPQRYEMPEGTPPAWGDFQYGLARAMSGGKAGIIDKGGNWVVQPVWADIGPPEARVLT
                     LKKK"
BASE COUNT          260 a          456 c          377 g          271 t
ORIGIN      
        1 tcttcttctt gagagtcagg acccgcgctt ccggtgggcc gatgtcggcc cagacgggct
       61 gcaccaccca attcccgccc ttatcaatga taccggcctt cccacccgac atcgcacggg
      121 ccaggccata ctggaaatca ccccaggccg gcggcgtccc ttcgggcatc tcgtaccgtt
      181 gtggaatgac gaatgcgccg gtcgtgtcca catagccgca cctgcccttg tccaccacca
      241 gtgcgagacc ctggctgaat ggaccgacat acccgtgctc ggccacgagt ttctcctcac
      301 ccttttcatt gatcaggccc attttgccgt tccggcgggc gcgcaccaga ggtgcaccaa
      361 agcgctcgat ccattcatac tcgaacggca caacgatccg acctgaaaca tcgatcgccc
      421 cgaagacatc gttccgggcc accacggcga aaccatcgtg gaaggtctgt gcatcatcga
      481 aggtgaacgg gatggccaca tcaccatcgg gatcgaggta gccgaactcg ccatccttgg
      541 ctgcgaatgc caatcctccg ctgtactccc cgatctcatc atatgccagg ccgacgatcg
      601 ttctccccga gcggtccacg accccggcgc gtccgtcccg ttccaccgtg gccaaccctt
      661 cactgaaggc gaggacatcc tcgtactcca ccggcaccac ctccttaccc gccttgctca
      721 ccgtacccac cgatccatca cggcccacct gcgcctgttg gccatgaaag ggttccgcga
      781 aatcatagac ggccttgatc cgttccacac ccagcgtatc aatataaccc cagtgtccat
      841 tccggcggaa tgggtggagc accaggccgg ccgtctcgta caatgcactc aactcttgaa
      901 cgaagggata gtccgggtaa tccttcaaaa aggcggtgat gctggctgcg gagaggtcct
      961 tggtgcgatc ctcgcagatg gcgcgccacg cctcatccac gtacgggttc ttcggatagc
     1021 ggcggatgaa tgcgtacaac tgctccgggg tgcgttcagg cgcgactgaa cgatagatgc
     1081 ccctgtaggc atcggccacc atggggcttc cgggatggtc cgcgatgaat ccttcgtact
     1141 cgacgagagt gccgttggga accgcttcgc ggaacgttgc ctcctgcatg tgcgcggtgg
     1201 cgagttccac ctgggccgca tcggggtgca tgtccagaaa gcggcggtag gcatccgccg
     1261 tgttctgcgc gtgtgcctcc tggaaggcga gctcatcccg ggcgatgatg gcctcggcta
     1321 tgaatgggct ccctgaaaat tggttgatat aggtctggta ccct
//
LOCUS       DAOQOC010000998         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_84045_length_1361_cov_3.351455, whole genome shotgun sequence.
ACCESSION   DAOQOC010000998 DAOQOC010000000
VERSION     DAOQOC010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84045_length_1361_cov_3.351455"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..736)
                     /locus_tag="PL106_13810"
     CDS             complement(119..736)
                     /locus_tag="PL106_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNA34198.1"
                     /translation="MVPPLLSIGQSSTSASYHDWTLLESRDGIVSYRGDTKVDGAVPT
                     KVELTIVAEPADVVAAITDFDGYKDWIPYCTSSEVLRTVNADECYAYYFISPPMVADR
                     DVVVHSVTIRAINGSYEVVIADTQDMLKEGPEGVIRMPHFLSHYWISVDGSGVTHITH
                     INETTLGGSIPEFLTSWSSHGQPLSTMQALKERILKHKTSALHQR"
BASE COUNT          265 a          396 c          399 g          301 t
ORIGIN      
        1 tcctcgggtc gcgcaggcat gatcacggcg gcgttcaggc tttcacccgt gaccgcccga
       61 ggctattgtc cggcacggct caaccggtgc atgccgctca cgctgcccga acaggcggtc
      121 agcgttggtg gagcgcagag gtcttgtgct tcaggatccg ctccttcaag gcctgcatgg
      181 tactgagcgg ttgcccgtgg ctggaccaac tggtgaggaa ctcgggaatg ctcccaccca
      241 gggtcgtttc gttgatgtgg gtgatgtgcg taacgccgga cccatccacc gagatccagt
      301 aatggctgag gaagtgcggc atgcggatga caccctccgg cccctccttc aacatgtcct
      361 gcgtatcggc gatcacgact tcgtaggatc cattgatcgc cctgatcgtg acgctatgga
      421 ccaccacatc ccggtcggcc accatgggcg gtgaaatgaa atagtacgcg tagcattcgt
      481 cagcgttcac cgttcgcaaa acctcgctgg acgtgcaata cggtatccag tccttgtacc
      541 cgtcaaaatc ggtgatcgcc gccaccacat ccgcgggttc cgccacgatc gtcaattcca
      601 ctttcgtggg aacagcccca tcaaccttgg tatcaccgcg ataggacacg atcccgtcac
      661 gactttccag gagtgtccaa tcgtggtacg atgcgctcgt gctggattgt ccgatggaca
      721 agagcggagg gaccatcgca aatgcggcca gagctccgga caacgatcga acgaacatca
      781 ggtggccggg ccgttggttc cgcatggcat cacgtcgtgt gtagtggtcg gcgtggagcg
      841 ggcctgccgg atcgggggat ggagccgggg ggttcctgga tcattcgcaa tgtggtggcc
      901 tttgttcgcg catgatcaaa gttatgcatc gttccattcc cgaccgttct ggcacaaaag
      961 gcccatgggt atttggttac ttcattcgac ctccgtcgta attcctattg aatgtcacgg
     1021 tgagcccgtc gaaccggttc ggcaggctca ccgtgacaag tctttttcat tcccctgagc
     1081 aattgggacg gaggtctatt gcaataatac tgaggccatg gggaagatcc ggccccaccg
     1141 tacctgggat acgctctcgg ccggatcgat ccgacggaag cgatcagcca gtttcttgaa
     1201 cgttccaaac gccattgtta cggctttgcg atccgatcag cggcaggcta tcacgcgggt
     1261 atgttttccc ttcccgcaag gtgatggtga catcctgatc gaaggggctg taaagcttgt
     1321 ccaccagtgc actgatgccg ttgagggtgc tgagcaccac c
//
LOCUS       DAOQOC010000999         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_84147_length_1360_cov_4.988506, whole genome shotgun sequence.
ACCESSION   DAOQOC010000999 DAOQOC010000000
VERSION     DAOQOC010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84147_length_1360_cov_4.988506"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PL106_13815"
     CDS             <1..298
                     /locus_tag="PL106_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNA34199.1"
                     /translation="NWQTAAQHQFYHGLALLGLAALEGKLATRIVSLVRIFFVLGVLC
                     FSGSLYLLATREMLGTDFLTRVLGPITPLGGLLFIAGWVVLLVGALHGLKRPGH"
     gene            603..>1360
                     /locus_tag="PL106_13820"
     CDS             603..>1360
                     /locus_tag="PL106_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNA34200.1"
                     /translation="MNEFGTKPANADLSNIGLKHPGDVYWNLEPAELIEHAVQNGEGI
                     FASSGALAVNTGEFTGRSPKDKFCVEDAKTKGTVWWGDINMPISAENFDRLHGKLTAY
                     LTGRDVYVKDAYACADPTYKLNLRVVAELPYSALFSSNMFLRPTAAELGSFTPEWHII
                     CAPGFMADGPADGIRQHNFAAIDFTRKMILIGGTGYTGEIKKGIFTVLNYVLPQERKV
                     LSMHCSANIGKSGDTAVFFGLSGTGKTTLSADPDR"
BASE COUNT          276 a          393 c          409 g          282 t
ORIGIN      
        1 caactggcag accgcagcgc agcaccaatt ttaccatggt ctggctttgc tgggactcgc
       61 cgccttggag gggaagctcg ctacgcggat cgtctccctg gtgcgtatct tcttcgtgct
      121 cggagtcctc tgcttcagtg gctccttgta cctgctggcc acgcgggaga tgttgggtac
      181 cgacttcctc acgcgcgtgc tggggcccat tacgccgctc ggaggcttgc tctttattgc
      241 gggctgggtg gtcctgctgg taggtgccct gcatgggctc aaacgcccag gtcattgaat
      301 ttgcttgatc aggcaagcag agcgacgggt cttcccagtg gcacaacgga tcctgctcgc
      361 ggaagcgatc agctatgaga taggtgcgtt cattcggctg ataatcaggt tatttatagc
      421 attcatttga tcttgaacgg taagctggaa gaatcgtagg agcttggtcg gctgtttggt
      481 tgtacattca ggaaagcacg gcgccacggc actatttcgg ttgatattcc tgtcgatcat
      541 catccatccc gccccaccct gtcccgctaa attcgcgggc gaaaaccaag aacaagcaat
      601 tcatgaacga gttcggaacg aaaccggcga acgctgacct gtccaatatc ggtctgaagc
      661 atccgggcga tgtgtactgg aacctcgagc ctgccgagtt gatcgagcat gcagtacaga
      721 acggggaggg cattttcgcc agcagcgggg cgctcgctgt gaacacgggc gaattcaccg
      781 gccggagccc caaggacaag ttctgtgttg aggacgcgaa gacgaagggt accgtgtggt
      841 ggggggatat caacatgccg atcagcgcgg aaaatttcga ccgcctgcat ggcaaactca
      901 cggcctacct cactggccgg gacgtgtatg tgaaggacgc gtacgcatgc gcggacccca
      961 cctacaagct gaacctgcgt gtagtggcgg agttgccgta cagcgcattg ttctccagca
     1021 acatgttcct gcgcccgacc gccgcggaac tggggtcctt cacccctgag tggcacatca
     1081 tctgtgcccc gggcttcatg gccgatggtc cggccgatgg catccgccag cacaatttcg
     1141 cagccatcga cttcacgcgg aagatgatcc tgatcggggg caccgggtac accggcgaga
     1201 tcaaaaaagg catcttcacc gtgctgaact acgtgctgcc gcaggagcgc aaggtgctca
     1261 gcatgcactg ctcggccaac atcggcaaga gcggcgacac ggcggtcttc ttcgggctga
     1321 gcggcaccgg caagaccacg ctcagcgccg atcccgatcg
//
LOCUS       DAOQOC010001000         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_84152_length_1360_cov_4.493487, whole genome shotgun sequence.
ACCESSION   DAOQOC010001000 DAOQOC010000000
VERSION     DAOQOC010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84152_length_1360_cov_4.493487"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            680..>1360
                     /locus_tag="PL106_13825"
     CDS             680..>1360
                     /locus_tag="PL106_13825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA34201.1"
                     /translation="MILQNWWFTTLLGTEEPIQPPLRKDIRADVLIVGAGAAGISAAL
                     HFVGKGLKVVVLERNILGGSTSGKSAGFLTPDSELELSQLIRRFGVHGARDLWSVPTK
                     GIERMRSTITDHGIDCDLQRQDSLFLGIGKSGWADIQDEMRARTALAFDQRLYDAPAL
                     AKIIGSTGYSGAVRYAETFGVNALRYCQGVKKILVGNGIEVHEASEVLSIEDHCARTH
                     LGSVTADQI"
BASE COUNT          273 a          398 c          441 g          248 t
ORIGIN      
        1 cagtgaacca tgtgcttgaa ttccgggacg cgttacaggc cgtacatccg gtatcgcaac
       61 tcacctgcga ggcgcagggc aggttcaacc attcgtaggt gatcgattgg gccgaagccg
      121 ccaagggcag gaccaacaag gggaggagcg gccatttcat tcgggccgcg aagttgcaca
      181 tcatgtggct accttcacct gatgatcgac agcagttgtg aacgatccgc cgatgcgcgg
      241 atttgtggat ataaaaggcc ttgccaacct caccagcgcc cgttcgcatc cgacggatcc
      301 cgcacgcccc gactgcccgg cggcggtttc atgcggtccg gtaacgaagt tccttgaagg
      361 cctgttccat cgtgcgggcc accaccacac gggtgccgtg gccctcgtgc aacagatccc
      421 ctgccgggga tgaccgccgt gcggacatcg gatccgtgat cggaggcaag gatcaggtgt
      481 tcggcgggat ggttcggggg catggatggc acccggaagc tggacccctc gtgttaaagg
      541 ttgacaacga cctgcaccat gaacgggcat gggggtggcc gggcccgtgt tcaagccgga
      601 atccacggtg atgcggggag ccgcggccta attttcgggg cggcatatcg ccttgaccgt
      661 acgctgatac catgataaaa tgatccttca gaattggtgg ttcacgaccc tgctcggaac
      721 tgaggaaccg atccaacccc cgctgcggaa agacatccgg gctgacgtgt tgatcgtcgg
      781 tgcgggagcg gcggggatct cggccgccct tcacttcgtg ggtaaagggc tgaaggtggt
      841 ggtgctcgaa cggaacatcc tcggcgggag cacatccggg aaaagcgccg gtttcctgac
      901 gccggacagc gagctcgaat tgtcccagct catccgccgg ttcggtgtgc acggtgcccg
      961 cgacctctgg agcgtgccca ccaaggggat cgaaaggatg cggtccacca tcaccgacca
     1021 tgggatcgat tgcgacctgc aacgccagga cagcctgttc ctcgggatcg gtaaaagtgg
     1081 atgggcagac atccaggacg agatgcgggc aaggaccgca ttggccttcg accagcggtt
     1141 gtacgacgcg cccgccctgg cgaaaatcat cggctccacc ggttattccg gtgcggtgcg
     1201 ctatgcggag accttcgggg tgaacgcgct gcgttactgc cagggcgtca aaaagatcct
     1261 ggtcgggaat ggcatcgagg tccacgaagc ctcggaagtg ctgtccatcg aagatcattg
     1321 tgccagaacc catctgggct cggtgaccgc cgaccagatc
//
LOCUS       DAOQOC010001001         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_84567_length_1357_cov_3.375576, whole genome shotgun sequence.
ACCESSION   DAOQOC010001001 DAOQOC010000000
VERSION     DAOQOC010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84567_length_1357_cov_3.375576"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PL106_13830"
     CDS             <1..194
                     /locus_tag="PL106_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA34202.1"
                     /translation="WFDDDFKVRVAKLDKEKPVRLYCAAGGRSEEAREVMREMGFKDV
                     KDLDGGISAWKKAGAPLVK"
     gene            complement(235..903)
                     /locus_tag="PL106_13835"
     CDS             complement(235..903)
                     /locus_tag="PL106_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNA34203.1"
                     /translation="MDEQRPLAGVDYLAGMFADEDLRRAFERDATQRNFVAGTEIMRP
                     GDTITHIPIVKRGSLRILAQDGEGRERYLYHILPGETCATSLTCCVSKRVSSVSAVVE
                     EDAELLMIPVRYVDEWMVYPEWRKYVNEIQAQRFQELLEAFEVVAFRKLDEQLWNYLV
                     KRSQATGENTLRLTHQEIADELVSPREVITRLLQQLRARGLVTLGRGHIELHPEAMDA
                     KSRG"
     gene            complement(907..1284)
                     /locus_tag="PL106_13840"
     CDS             complement(907..1284)
                     /locus_tag="PL106_13840"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNA34204.1"
                     /translation="MIRRNTALHSILHLKCPYCHEGAFFVSHPYDLSRAGDIHDKCPE
                     CGGRFNIEPGFYYGAMYVSYAIGVAACVTVWVASLVLAPDLPLRVLIMLIAGTMLLGG
                     PLFYALSKIIWAHLFLRKGSERH"
BASE COUNT          273 a          417 c          413 g          254 t
ORIGIN      
        1 attggttcga cgacgatttc aaggtgcgtg tggcgaagct ggacaaggag aagccggttc
       61 ggctgtactg cgccgcgggc ggccggagcg aagaggcgcg cgaagtgatg cgcgagatgg
      121 gcttcaagga cgtgaaggac cttgacggtg gcatctccgc ttggaagaaa gccggtgccc
      181 cgttggtgaa gtaaccggtt gccgcggagg ataacggtac cccatggtcc catctcaccc
      241 ccggctcttc gcgtccatcg cctccggatg caattcgata tggccgcgcc ccagcgtcac
      301 cagcccccgg gcgcgcaact gctgcaacag gcgggtgatg acctcgcgcg gtgagaccag
      361 ttcatcggcg atctcctggt gggtgaggcg cagggtattc tcccctgtgg cctgcgaacg
      421 tttcaccaga tagttccaga gctgctcgtc gagtttgcgg aaagcgacca cttcaaaggc
      481 ctcgagcagt tcctggaaac gctgggcttg tatctcgttc acgtacttgc gccattcagg
      541 gtacaccatc cactcatcca cgtaacgcac ggggatcatc aacaattcgg cgtcctcttc
      601 caccacggcg cttacactgc tcaccctttt gctcacacag caggtgagcg aggtggcgca
      661 tgtctcgccg gggagtatgt gatagaggta acgctcgcgc ccctccccgt cctgggcaag
      721 aatgcgcaaa ctgcctctct tcacgatggg gatgtgggtg atcgtgtcac ccggccgcat
      781 gatctccgtt ccggcgacaa aattccgctg cgtcgcatcg cgctcgaacg cgcgccggag
      841 atcctcatcg gcgaacatgc ccgcaaggta gtccactccg gcgagcggcc gctgttcgtc
      901 caacgttcaa tgccgttcac tccccttccg caggaacaag tgcgcccaaa tgatcttgct
      961 caatgcgtag aaaagtggtc ctccgagcag catggtgccc gcgatcagca tgatgagcac
     1021 gcggagtgga agatcggggg ccaggaccag cgaggccacc cacacagtga cgcacgccgc
     1081 cacgccgatc gcgtatgaca cgtacatggc accataatag aaacccggct cgatattgaa
     1141 ccggccacca cattccgggc acttatcgtg gatgtcgccg gcccgggaca ggtcgtacgg
     1201 atgcgacacg aagaacgccc cttcgtggca gtatgggcat ttgaggtgga gaatgctgtg
     1261 caacgcggta ttcctgcgga tcatggggcg aagatcgctc accgctcagc ggagaggtgg
     1321 ggcgaacaat tcctggaccg ccctatccgc gctcaag
//
LOCUS       DAOQOC010001002         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_84692_length_1356_cov_3.811683, whole genome shotgun sequence.
ACCESSION   DAOQOC010001002 DAOQOC010000000
VERSION     DAOQOC010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84692_length_1356_cov_3.811683"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PL106_13845"
     CDS             complement(<1..710)
                     /locus_tag="PL106_13845"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNA34205.1"
                     /translation="MQPNNGELKPLDHWIRVCKEHAFDLQFTRARKWKEEHADGVVVG
                     YMPVYFPREVIHAAGGLPVGIFGGGDRKQIIKGDAYYQSYICHIPRSIIDLVLDNHTD
                     HFDGYIFPSICDVLRNLSGIFKLNKLGKFVKYMDFPQNFLPEVGGVFYQQEMQHVMHY
                     IKEINGKEVTPEALNRSIALYNTNRRLIARIYDIRQDHPWRMTLEELYYVVRAGTVMP
                     VEEHNAILEQVCTHLERET"
BASE COUNT          259 a          408 c          419 g          270 t
ORIGIN      
        1 gtttcacgtt ccaggtgggt gcatacctgc tccaggatgg cattgtgctc ttccaccggc
       61 atcaccgtgc cggcgcgcac cacgtagtac agttcctcca gggtcatgcg ccaagggtga
      121 tcctgacgta tatcatagat ccgggcgatc aggcgccggt tggtattgta cagcgcgatg
      181 gagcggttca gggcctcggg cgtcacctcc ttcccgttga tctccttgat gtagtgcatc
      241 acgtgctgca tctcctgctg gtagaacacg ccgcccacct cgggcaggaa gttctgcggg
      301 aagtccatgt acttcacgaa cttgcccagc ttgttcagct tgaagatgcc cgagaggttg
      361 cgcagcacgt cgcagatgct cgggaagatg tagccgtcga agtggtcggt gtggttgtcc
      421 agcaccaggt cgatgatgct gcgcgggatg tggcagatgt acgactggta gtaggcgtcg
      481 cccttgatga tctgcttgcg gtcgcccccg ccgaagatgc ccaccggcag gccgccggcg
      541 gcgtggatca cctcccgggg gaagtacacg ggcatgtagc ccaccaccac gccgtccgca
      601 tgttcctcct tccatttccg cgcgcgggtg aactgcaggt cgaaggcgtg ctccttgcag
      661 acgcggatcc agtggtccag gggtttcagt tctccgttgt tcggttgcat ggtgaagggg
      721 attcgggatc caacatcgga tagctccgcc cgccgggtcc catggccatg ggcgcgcgca
      781 caggcccatc gaagcgggcg cacttcaccc ctgcccgcag cggcaccgtt tgcggcctgt
      841 aagctgaccg gacatgcatg atcgatgttg gatctgagcg aagcaatcag atcgggggga
      901 tccggaaaat gatttatatc ataaaaagca cgagttgctg gcacacgccc ggtagccgat
      961 cggacgcggc cgccgacccc ccccgtgcgc gcaagcgggc ttctgtccaa agcttcagca
     1021 gccggttatt cggcaccgcc ccgctcgaaa cggagcaaga tcccggatcc tcttgatgta
     1081 cagcgctgga cctatgtgat gctcgtcgtt cgtccaggcc cggacaagaa gttcagaggc
     1141 cgatcgaaca tgtcagcagg ggccattcca tccgcgccgg gctcatcagg aacagcatca
     1201 cggcaatggg agggaacaca attggtacag gccccaagag cctcaactct tcgggcagtg
     1261 acattggact gtgatgcgac ctgctgtcga gcatgtgccc ggcaggtgat ggggcagcac
     1321 gatcggactt cagccgtata ccgatcagta caacca
//
LOCUS       DAOQOC010001003         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_84719_length_1356_cov_3.079939, whole genome shotgun sequence.
ACCESSION   DAOQOC010001003 DAOQOC010000000
VERSION     DAOQOC010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84719_length_1356_cov_3.079939"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..610)
                     /locus_tag="PL106_13850"
     CDS             complement(116..610)
                     /locus_tag="PL106_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34206.1"
                     /translation="MTDMTMDAPKRPTLLTVLCILSFIGGILGLWDGYKNAFTDAPQK
                     ALEEAKAQVQESMDQLGADNPMAGMMEGMIATAEKGVEHAKTLGYSNLAFSLISLIGV
                     WMMWNLKKTGFWLYLLASIGGLAVMFSVLGGGGLFANITSILAVVITAVFIILYGVNL
                     KHMR"
     gene            841..>1356
                     /locus_tag="PL106_13855"
     CDS             841..>1356
                     /locus_tag="PL106_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA34207.1"
                     /translation="MSYDVIVLGSGPGGYVAAIRASQLGLKTAIVEKEALGGICLNWG
                     CIPTKALLKSAQVFEYINHARDYGINVGAGAPDMKAIVQRSRDVAKGMSNGVQFLMKK
                     NKIDVIMGTGLLQPGRKIEVTGADGKTQVVEGKHIIIATGARSRALPNLPQDGKKIIG
                     YREAMVLPEQPK"
BASE COUNT          299 a          397 c          425 g          235 t
ORIGIN      
        1 atcgtactgg aattttttga tgcccgtgat gccggcagat ccacctgcga tggaagggat
       61 gcggcaaaat gggagtggcg atcccgctta cagcccgtga cgaggtacgg gacgttcagc
      121 gcatgtgctt cagattcacc ccgtagagga tgatgaacac cgcggtgatg accaccgcaa
      181 gaatggaggt gatgttggcg aacagtcccc caccaccaag tacggagaac atcacggcca
      241 gaccaccgat agaggccaga aggtacagcc agaagccggt cttcttcagg ttccacatca
      301 tccaaacgcc gatcaagctg atcagcgaga aggccaagtt ggagtagccc aaggtcttcg
      361 cgtgttccac acccttctcg gccgtggcga tcatgccttc catcatgccg gccatgggat
      421 tgtcggctcc gagctggtcc atggattcct gcacttgggc cttcgcctct tccagtgcct
      481 tctgcggagc atcggtgaaa gcgttcttgt agccgtccca cagtccgaga atgcctccga
      541 tgaagctgag gatgcagagc acggtgagca gggtggggcg cttgggcgcg tccatggtca
      601 tgtcggtcat gtggtaggtg tttggatggt cgaagctaac cgtccgttcc atacggccaa
      661 acacgttctg ggacttggag gatgaacgac cggagccgct ccgttggaga attcgtggca
      721 ccgaccgctt tacggggcga aggcggatcg caccaagaac gaagctgagc gggatcgcca
      781 caccgagcgg agccgcggcc taccttcgcg gcccatccgg aaaatacgga cgcacccaac
      841 atgagctacg acgttatcgt tctcggcagc ggccccggcg gctacgtggc cgccatccgt
      901 gccagccaac tcggtctgaa gaccgccatc gttgaaaagg aagccttggg cggcatctgc
      961 ctcaattggg gctgcatccc caccaaagca ctgctcaaga gcgcgcaggt gttcgaatac
     1021 atcaaccacg ccagggacta cggcatcaac gtgggtgcgg gtgcgcccga catgaaggcg
     1081 atcgtgcagc gcagccgcga tgtggccaag ggcatgagca acggcgtgca gttcctcatg
     1141 aagaagaaca agatcgacgt gatcatgggc acggggctgt tgcagcccgg tcgcaagatc
     1201 gaggtcaccg gcgccgacgg gaagacgcaa gtggtggaag gcaagcacat cattatcgcc
     1261 acgggcgcgc gcagccgggc actgcccaac ctgccgcagg acggcaagaa gatcatcggc
     1321 tatcgcgagg ccatggtgct gcccgagcag cccaag
//
LOCUS       DAOQOC010001004         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_85249_length_1352_cov_4.175790, whole genome shotgun sequence.
ACCESSION   DAOQOC010001004 DAOQOC010000000
VERSION     DAOQOC010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85249_length_1352_cov_4.175790"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..756
                     /locus_tag="PL106_13860"
     CDS             148..756
                     /locus_tag="PL106_13860"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNA34208.1"
                     /translation="MVITEQINLFIAEQAEWQRKPLARLRQLIHTADPNVEETWKWNC
                     PHFDHAGIMIGLNAFKEHIAVWFHKGALIKDPKKLFEPLAKGEEKGMRTYKLHEGDAI
                     NEAAFTDLVKQAVALNEKGTKLTDAKPARKALETPEDLEQVLHKDPTAWANWESFSTS
                     HKREYIEWVTDAKQEETRKRRIAQALEKIREGESQNEKYKVH"
     gene            852..>1352
                     /locus_tag="PL106_13865"
     CDS             852..>1352
                     /locus_tag="PL106_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA34209.1"
                     /translation="MDIRIENLTKRYGPQVAVDDISFRVGAGEVLGFLGPNGAGKSTT
                     MKMICGLIAPNAGSIAVGGKEVAEEPDAVRGMIGYLPESNPLYDDMPVLDLLKFAARI
                     QKVPEDKVLPRIREMVNACGLGPEKHKLVGELSKGYRQRVGLAQAMVHDPQVLILDEP
                     TTGLDPN"
BASE COUNT          314 a          400 c          436 g          202 t
ORIGIN      
        1 gccattacct tgagcgggga ggcgaagaag tgaactgacg cccggcgcgg atcatgggga
       61 tgatcgatcc gaaggtcatt cgcaccatat tcgccccgag aagtccgttg tccggaaggg
      121 ccgggccgaa gtcaactccc cttgccaatg gtcatcacgg agcagatcaa cctcttcatc
      181 gccgagcagg ccgaatggca gcgcaaaccg ctggcccggc tgcgtcagct catccacacg
      241 gccgatccca acgtggagga aacctggaaa tggaactgcc cgcacttcga ccacgcgggc
      301 atcatgatcg ggctcaacgc gttcaaggag cacatcgcag tatggttcca caagggcgca
      361 ttgatcaagg accccaagaa gctcttcgag ccactggcga aaggcgagga aaagggcatg
      421 cgcacgtaca agctgcacga gggtgatgcg atcaacgagg ccgcgttcac cgatctggtg
      481 aagcaggccg tggccctgaa cgagaaaggc accaaactca ccgatgccaa gccggcccgc
      541 aaggcgctgg aaactcccga ggacctggag caggtgctgc acaaggatcc caccgcatgg
      601 gccaactggg agagcttttc caccagccac aagcgcgagt acatcgaatg ggtcaccgat
      661 gccaagcagg aggagacgcg caagcgccgg atagcccagg ccctggagaa gatccgcgaa
      721 ggtgagagcc agaacgagaa gtacaaagta cattgatgag aggtgagagg cgagaggcga
      781 gtggggagtg accctgtgag gtcagcatgc tgcctctcgc ctctcacctc tcgcctctca
      841 cctctctctc catggacatc cggatcgaga acctgaccaa gcggtacggc cctcaggtgg
      901 cggtagacga tatcagtttc cgggtaggtg cgggcgaggt gctgggtttc ctgggtccga
      961 acggtgcggg caagagcacc accatgaaga tgatctgcgg gctgatcgcc ccgaacgctg
     1021 gcagcatcgc cgttggcgga aaggaggtgg ccgaggaacc cgatgccgtg cgcggcatga
     1081 tcggctacct gccggagagc aacccgttgt acgacgacat gccggtgctc gacctgttga
     1141 aattcgcggc tcggatccag aaggtacccg aggacaaggt ccttccacgg atccgggaaa
     1201 tggtgaacgc ctgcggcctg ggtcccgaga aacacaaact cgtgggcgag ctgagcaaag
     1261 gctaccggca acgcgtgggg ctggcgcagg ccatggtgca tgacccgcag gtcctgatcc
     1321 tggacgagcc gaccaccggc ctggacccga ac
//
LOCUS       DAOQOC010001005         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_85485_length_1351_cov_2.192130, whole genome shotgun sequence.
ACCESSION   DAOQOC010001005 DAOQOC010000000
VERSION     DAOQOC010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85485_length_1351_cov_2.192130"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PL106_13870"
     CDS             complement(<1..245)
                     /locus_tag="PL106_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNA34210.1"
                     /translation="MIPFSPPRIDDATIAAVTAVLRSGWITTGPNVKEFEKRLADYCQ
                     VPRVIAMNSWTNAAELVLRWYGIGPGDEVIVPSYTYCA"
     gene            403..1245
                     /locus_tag="PL106_13875"
     CDS             403..1245
                     /locus_tag="PL106_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA34211.1"
                     /translation="MALHRIVIEPGTADRHYWRDLWTYRGLFWFLAWRDILVRYKQTV
                     IGIAWSVLRPLLTLGAMALVGWLFNSHVPEGVPRLLLVAAATLPWTFFSTAFSEASNS
                     LIANSNLLTKVYFPRLIVPASTLLVCLIDLLISFAILLVLLAVYRYVPDFRILFLPLF
                     TLLAIVTALGSGLLIAAINVKYRDFRYVVPFMVQFGLYVSPIAFTSTDIYSNERIPMA
                     VRTLYSMNPLVGVIDGFRWCILGGTSPIHMPGFILSVGISLLLLLLGVRYFRRMERSF
                     ADVI"
BASE COUNT          231 a          448 c          386 g          286 t
ORIGIN      
        1 gcgcaatacg tgtacgacgg tacgatcacc tcatcgcccg ggccgatccc gtaccagcgc
       61 aggacgagtt cggccgcatt ggtccaactg ttcatggcga tcacgcgagg cacctggcag
      121 tagtcggcca aacgcttctc gaactccttc acattggggc cggtggtgat ccagccggag
      181 cggagcaccg cggtcaccgc cgcgatggtc gcatcatcaa ttcggggcgg ggaaaagggg
      241 atcatgcgct gttgaacgcg cgaagatcgt tcctcgcgta caacgacggc atgtcgaaga
      301 cacagaagtc cctctgtgcc actcccggaa cggtcttcca gccgcacagg gaccgcggtc
      361 ggcccgtctt cgcggctgct gggtagcttc gcgccgcgac cgatggccct gcaccgcatc
      421 gttattgaac cgggaacagc cgaccgccac tattggcgcg atctgtggac ctaccgtgga
      481 ctcttctggt tcctggcctg gcgcgacatc ctggtacggt acaaacaaac ggtgatcggt
      541 atcgcatgga gcgtgctgcg tcccctgctc accttgggcg ccatggccct ggtgggctgg
      601 ttgttcaaca gccacgtgcc cgaaggagtg ccccgcctcc tcctggtggc cgcggccacc
      661 ttgccgtgga ccttcttcag cacggctttc agcgaagcct ccaacagcct gatcgcgaac
      721 agcaacctgc tcaccaaggt gtatttcccc cggctcatcg tgccggccag caccttgctc
      781 gtctgcctga tcgacctgct gatctccttc gccatcctgc tcgtgctcct ggcggtgtac
      841 cgttacgtac cggacttcag gatcctcttc ctgccgctct tcaccctgct ggccatcgtc
      901 acggcattgg gcagcggatt gctcatcgcc gcgatcaatg tgaagtaccg cgacttcagg
      961 tacgtcgtgc ctttcatggt gcaattcggg ctgtacgtct cccccatcgc cttcaccagc
     1021 acagatatct actctaatga aaggattccg atggccgtcc ggacgttgta ttcaatgaat
     1081 ccgctcgttg gcgtgatcga cgggttccgc tggtgcatcc tgggcggcac ctcccccatc
     1141 cacatgcccg gcttcatcct gtcggtcggc ataagcctgt tgctcctcct gttgggcgtg
     1201 cgttacttcc gtcgcatgga acgctccttc gccgacgtga tctgaaccat gggcgaaccg
     1261 atcatccgcg tggaagggct cagcaagagt tacgtgctgc gacatcagca gcgcgaacgg
     1321 tacaccgcac tgcgcgatgt gatcgcggcg a
//
LOCUS       DAOQOC010001006         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_85560_length_1350_cov_3.091892, whole genome shotgun sequence.
ACCESSION   DAOQOC010001006 DAOQOC010000000
VERSION     DAOQOC010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85560_length_1350_cov_3.091892"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PL106_13880"
     CDS             complement(<1..1183)
                     /locus_tag="PL106_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34212.1"
                     /translation="MERTLLLWMMALNGIIAQAQAPFANVYDAPSDTTSPWYTGGRVA
                     LRPDSGFTVLGHAGSNSPDRKIIYVDRIDADGAPLQHDRYHFAQDTVYLYNCFTRSDA
                     GSLLIGTVRDRDLFMMHVADDGTCVSALIYDDPDPLVISSRWHRAVPMPDDSLLLVGS
                     ASGAGGSKGLIAKCDTNGMIASGTYLQINNAATAFLGAEALANGDVLAWGGVNGPMGG
                     SNVARIAPDGTVIWAERVREIPSSGMQISGAVELSDGTLLLGMSYVPAGQTTMHPALL
                     ALASDGSLIDWKFYPDMISGVYGAIPANNDGVLMVGSGPGALRFLSVDATGAIATAGE
                     LNATMSQQAFIADATGAPVISGLTPVLPRRPAMIRGTGAFAACGVVPLTSTVQSLTPI
                     IA"
BASE COUNT          241 a          421 c          463 g          225 t
ORIGIN      
        1 cggcgatgat gggggtgagg gattgcacgg tggaggtgag gggcaccacg ccgcatgcgg
       61 cgaacgcacc ggtgccgcgg atcatggcag ggcgtcgcgg cagcacgggc gtgagaccgg
      121 agatcaccgg ggcgccggtc gcatcggcga tgaaggcctg ttgggacatc gtcgcgttca
      181 gctctcccgc tgtcgcgatg gcgccggtcg cgtccacgga aaggaagcgc aaggcacccg
      241 gcccggatcc gaccatcagc acaccatcgt tgttcgccgg aatggccccg tatacgccgg
      301 agatcatatc gggatagaac ttccaatcga tcaggcttcc atcggacgcc aaggcgagca
      361 acgcagggtg catggtggtc tggcccgctg gtacatagga catgccgagc agcagcgtac
      421 cgtcgctgag ctccaccgca ccgctgatct gcatgccgct ggatgggatc tccctcaccc
      481 gttccgccca gatcaccgtg ccgtcaggtg cgatgcgggc gacgttggaa ccacccattg
      541 ggccgttgac gcccccccac gccagcacat cgccgttcgc aagggcctcg gcgccaagga
      601 acgctgtggc cgcgttgttg atctgcaggt aggtgccact ggcgatcatc ccattcgtgt
      661 cgcacttcgc gatcagaccc ttggaaccgc cggcccccga tgcactaccg acgagcagca
      721 gggaatcgtc gggcatgggt acggccctgt gccacctgct cgagatgacc agcgggtcgg
      781 ggtcgtcgta gatcaacgcc gacacgcatg tgccatcatc ggccacgtgc atcatgaaca
      841 ggtcgcggtc gcgaacggtc ccgatgagga gggagcccgc gtcggagcgc gtgaagcaat
      901 tgtagaggta caccgtgtct tgggcgaagt ggtaccggtc atgctgcaag ggcgcaccat
      961 cggcgtcgat ccggtccacg tagatgatct tccggtcggg gctgttcgag ccggcgtgac
     1021 caaggaccgt gaagccgctg tccggtcgga gggcgaccct gccgccggtg taccatggag
     1081 aggtcgtatc ggacggtgca tcgtagacgt tggcgaaggg ggcctgggcc tgggcgatga
     1141 tcccgttcag ggccatcatc cagagaagga gggtgcgttc catggggctg gtcttgctgt
     1201 atgacgagcg atgcacgcct gggtggcctg ctcgccgcac gacggagttc cgctcaaggt
     1261 agcggttcca tcagcccgcc acgttcaccc cgatcagccg gccgatgccg aaggtgatcg
     1321 ccgcggccgc aagtccgaag aacacctgtc
//
LOCUS       DAOQOC010001007         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_85561_length_1350_cov_3.064865, whole genome shotgun sequence.
ACCESSION   DAOQOC010001007 DAOQOC010000000
VERSION     DAOQOC010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85561_length_1350_cov_3.064865"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PL106_13885"
     CDS             complement(<1..1131)
                     /locus_tag="PL106_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34213.1"
                     /translation="MNCLQLHAWAQAEGCNDPLLILDISPPTPLVSVLNLTTNSPILI
                     GDPSQAETLSLTVNNPGTDPVDGMVLSVALPDCWTMSTPAPGWTVTPGQPDETWSYAL
                     PVLSPGQSPTFYLPLQVGSTCPSGAIQIQTWIHHATMGSCASCNDPLDEAQVVVTIND
                     DHGCEITATASTTYSCAPPEDNDCAVLPIQEDEPLYEDSAERFPALTMSFNGITGDFI
                     FENGWGIFNEYEPGKWQLIGELSETSEPDHLIWIGLFLLGPFTDPQDLPDVPMPIEVG
                     GFGDPGTWTYYVLDDQAPNRILDIGTQPPSQYLITQSGTLGFQHGLGANTHNSHQGLF
                     GWLHVNGQDVALSIELDCERDLPNAECSGTATIELTEPPGPVI"
BASE COUNT          278 a          376 c          412 g          284 t
ORIGIN      
        1 tatgactggt cccggtggtt ctgtcaactc tatggtagca gtacccgaac actccgcatt
       61 cggcagatca cgttcacagt ccagctcgat cgagagcgca acatcttgtc cgttcacatg
      121 cagccatccg aagagaccct gatgcgagtt gtgcgtgttc gcacccaggc cgtgctggaa
      181 gcccaaggta ccggactggg tgatgaggta ttgagaaggt ggttgcgtcc caatgtccag
      241 aatacggttc ggtgcctggt cgtccaacac atagtacgtc catgtcccgg ggtctccgaa
      301 accgccgacc tcgatcggca tcggcacgtc aggcaggtct tgagggtccg tgaacgggcc
      361 gagtaaaaag agcccgatcc agataagatg atcaggttcg gaggtttcgc tcagctctcc
      421 gatgagttgc cacttacccg gttcgtactc gttgaagata ccccatccat tttcgaagat
      481 gaagtctccg gtgatgccat tgaacgacat cgtcagcgcc gggaagcgct ccgcactgtc
      541 ttcatagagc ggttcatctt cttggatggg cagcactgca caatcattgt cctcgggtgg
      601 ggcgcaagag tatgtggtgc tggcggttgc ggtgatctcg catccgtggt cgtcattgat
      661 ggtgaccacc acctgcgcct cgtccaaagg gtcgttgcag gaggcacagc tacccatggt
      721 ggcatggtgt atccaagttt gaatttggat ggcaccgctt ggacaggtac tgccaacctg
      781 cagcggcaag taaaaggtcg gactctgacc gggtgacaac acaggcagcg cgtaactcca
      841 cgtctcatcc ggttgccccg gtgtcaccgt ccagccgggg gctggtgtgc tcatggtcca
      901 acagtccgga agggccaccg agagcaccat tccgtcgacc ggatccgtgc ccggattgtt
      961 gaccgtgaga ctcaacgtct cagcctgtga tggatcaccg atgaggatcg gcgagtttgt
     1021 ggtcaggttc agaacagaaa caagcggcgt gggcggcgag atgtccagga tgagcagtgg
     1081 atcgttacag ccttcagcct gcgcccatgc atgcaactgg aggcagttca catcgcaggt
     1141 cggagttgtg aagtgcgctt tgacccgtag tcggctatcg gattcggcgc attggccgct
     1201 gagcgcaccg cacaaggccg aggacatgcc cgggatggtg ttcaccggaa tttcccagac
     1261 ccagcaatcc gctgaaggcg agcccgggtc ttgtgacacc ggcggacagg atcccgggac
     1321 gagatcaccg atggccatcg cgttcaattg
//
LOCUS       DAOQOC010001008         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_85660_length_1349_cov_3.389490, whole genome shotgun sequence.
ACCESSION   DAOQOC010001008 DAOQOC010000000
VERSION     DAOQOC010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85660_length_1349_cov_3.389490"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PL106_13890"
     CDS             complement(<1..1341)
                     /locus_tag="PL106_13890"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34214.1"
                     /translation="MTIPPNTPPGTGYRIRVNSTQPAYTGSDNGFDITIDGAAPSAVI
                     SADGPLEFCGGGSVTLNADPVAGTYQWQLDGTDIPDANNAAYDAVAAGVYALVVTNAC
                     GADTSDAITVTVGDPPVYTFDQPAYLSCDGVPVTITSVDGSGQSDLTYAWYLNNALLA
                     GEDDPSLLVGEAGSYSLTVTNTVTGCSYTADPVEVSIDQVATPVIQADGPAEFCSGDS
                     VGLSVDAVPGVSYQWSVDGTTIDGATATTIIVQGSGAYTVTATSNGCVSGSSDPMTIL
                     VNQTPAIPVINALDGTSYCEGGSTVLDAGTNTDITYQWILNDTAIVDGTNAQWTAVEP
                     GAYTVVVTADGGCMATSEVITITENALPAQPVIMQDGDQLTTSGEGGFQWYFEGQPIS
                     GATDSTYMPTENGAYNVAFTDANGCTSLSDAYAWVSTRINISVGQGIALWPNPSH"
BASE COUNT          238 a          404 c          456 g          251 t
ORIGIN      
        1 atgcgagggg ttcggccaga gcgcgatgcc ctggcccacg gatatgttga tccgggtact
       61 gacccaggcg taggcgtcgc tcaggctggt gcaaccgttg gcatcggtga aagccacgtt
      121 gtacgcaccg ttctccgtgg gcatgtacgt gctgtccgtg gcgccgctga tgggctggcc
      181 ctcgaagtac cactggaagc caccttcacc cgaagtcgtt aattgatccc catcctgcat
      241 gatcaccggc tgggcgggca atgcgttctc cgttatggtg atgacctccg aagtggccat
      301 gcaaccgccg tcggccgtga cgaccaccgt gtacgcgccg ggttccacgg ctgtccattg
      361 cgcgttggtc ccgtcaacaa tggccgtatc gttcaggatc cactggtagg tgatgtcggt
      421 gttcgtgccc gcatcgagca cggtggatcc gccttcgcag taggaggtgc cgtccagtgc
      481 gttgatcacg gggatggccg gggtctggtt gaccaggatg gtcatcggat ccgaggatcc
      541 cgacacgcaa ccgttgctgg tggctgtcac ggtatacgct ccggacccct gcacgatgat
      601 cgtggtggcg gtggcgccat cgatggtggt accgtccacc gaccactggt agctgacacc
      661 gggaaccgca tccacgctca gcccgacgga atcacccgag cagaattcgg cggggccatc
      721 ggcctggatc acgggagtgg ccacctggtc gatggagact tctaccgggt cagcggtata
      781 ggagcacccg gtcacggtgt tcgttacggt aagcgaataa ctgccggcct cgcccacgag
      841 cagcgaagga tcatcctcgc cggccagcaa tgcgttgttc aggtaccagg cgtacgtcag
      901 gtcactctgt ccggagccgt ccaccgaggt gatggtcacg ggaacgccgt cgcaggagag
      961 gtaggcgggc tggtcgaagg tgtacaccgg cggatcgccg acggtcaccg tgatggcatc
     1021 agaggtatcg gcaccgcagg cattggtgac cacgagcgcg tacacaccgg cggcgacggc
     1081 atcgtacgcg gcattgttcg catcagggat atcggtgcca tcgagctgcc actggtaggt
     1141 gccggccacc ggatcagcgt tgagcgtcac ggacccacca ccacagaatt ccagcgggcc
     1201 atcagcggag atcacggccg agggggccgc gccgtctatg gtgatgtcga agccgttgtc
     1261 cgaaccggtg taagccggct gggtcgagtt cacgcggatg cggtaaccgg tgcccggcgg
     1321 cgtgttcggc ggaatggtca cggtcatgc
//
LOCUS       DAOQOC010001009         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_85902_length_1347_cov_4.026316, whole genome shotgun sequence.
ACCESSION   DAOQOC010001009 DAOQOC010000000
VERSION     DAOQOC010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85902_length_1347_cov_4.026316"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PL106_13895"
     CDS             complement(<1..241)
                     /locus_tag="PL106_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34215.1"
                     /translation="MIPTNDERLRQLLLGQQQLMEEVEGILAEEQRKDDHLSAAVLSS
                     RGDHINHILRPDPDRIFSEKDIQRLCVAYKLRFLDA"
     gene            complement(281..664)
                     /locus_tag="PL106_13900"
     CDS             complement(281..664)
                     /locus_tag="PL106_13900"
                     /inference="COORDINATES: protein motif:HMM:NF020550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HNA34216.1"
                     /translation="MRFLHVKFPGKKKEIVEVAISHQARVKFMTAREFKLYSNSRTHT
                     FFGGTFKKGTVRFVVPFDSVWTVVVEKGTASAPVDVTARCSVLPPDRDALSTVALDAP
                     AHVRAIQANDQQQVEALSEDDQGQG"
     gene            complement(727..>1347)
                     /locus_tag="PL106_13905"
     CDS             complement(727..>1347)
                     /locus_tag="PL106_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA34217.1"
                     /translation="LERVPALPFLRGIELRKFMLRQLSITEIFKNDRLAKSDLVVMGS
                     HGARGFRGVVGSNTQKVVRIAPMPVLVIKHQIDDFTVNDLVYASNFTETDIMKFDAFL
                     PVLEIFDPKVHLVKVNTPKAFERSEDTYKAIDRFLQRHELRKFTSTIYNDLSIEEGIL
                     NFARGIDADMIAMATHGRTGFFQVVNGSLTEDIVNHTTFPVLSVKL"
BASE COUNT          252 a          384 c          407 g          304 t
ORIGIN      
        1 cggcatcgag gaatcgcaac ttgtacgcca cacacagccg ctggatatcc ttctcactga
       61 agatgcggtc gggatcgggg cgaaggatgt ggttgatgtg gtcgccccgg gaggaaagga
      121 cggccgcgct gagatgatca tccttgcgtt gctcctctgc gaggatcccc tccacctcct
      181 ccatcaattg ctgctggccc agaagaagtt ggcgcagccg ctcgtcgttg gtggggatca
      241 tggttctgtg gaacaacggc cctttcaggc gatccttgtg tcaaccttgg ccttggtcgt
      301 cctcgctgag cgcttcgacc tgctgctggt cgttggcctg gatggcacgt acgtgcgccg
      361 gtgcatcgag ggccacggtg ctgagcgcat cgcggtccgg cggcaacacg ctgcaacggg
      421 ccgtcacatc cacaggtgct gaagcggttc ctttctccac caccaccgtc cacacactgt
      481 cgaacgggac tacgaatcgt acggtacctt ttttgaaggt gccaccgaag aaggtgtgcg
      541 tacgggagtt gctgtagagt ttgaactcgc gggcggtcat gaacttcaca cgggcctgat
      601 ggctgatcgc gacctcgacg atctccttct tcttgcccgg gaatttcaca tgcaggaatc
      661 tcatgcggcg tgccttggta tcggggctaa tttagaggga cggatcgatc ggatcgcggt
      721 tggaggtcaa agtttcacac tgagcaccgg gaatgtggtg tggttcacga tatcctcggt
      781 gaggctgccg ttcaccacct ggaagaagcc cgtgcggccg tgtgtggcca tggcgatcat
      841 atcggcatcg atgccccggg cgaaattgag gatgccctct tcgatgctca ggtcattgta
      901 gatggtgctg gtgaacttgc gcaattcgtg ccgctgcagg aaccggtcga tggccttgta
      961 ggtgtcctcg ctccgctcga acgctttcgg ggtgttcact ttcacgagat gcaccttggg
     1021 gtcgaagatc tccagaacgg gcaggaacgc gtcgaacttc atgatatcgg tctccgtgaa
     1081 gttgctggcg tacaccaggt cgttcaccgt gaagtcatcg atctggtgct tgatcaccag
     1141 gacgggcatg ggcgcgatgc gaaccacttt ttgggtattg ctgcccacca cccccctgaa
     1201 accgcgggcg ccatggcttc ccatcaccac cagatccgac ttcgccaagc gatcgttctt
     1261 gaagatctcc gtgatcgaga gttgcctcag catgaatttc ctcaattcga taccgcgcag
     1321 gaacggcaag gcgggcacac gctccaa
//
LOCUS       DAOQOC010001010         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_86409_length_1343_cov_4.785714, whole genome shotgun sequence.
ACCESSION   DAOQOC010001010 DAOQOC010000000
VERSION     DAOQOC010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86409_length_1343_cov_4.785714"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..>1343
                     /locus_tag="PL106_13910"
     CDS             207..>1343
                     /locus_tag="PL106_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNA34218.1"
                     /translation="MKKKDRKIFVLDTSVILYDHSAISCFQEHDVAIPIQVLEELDTF
                     KKGNDTINFEAREFIRHLDGIAKGDLLTDWTPLNGSTTGKFKVVAKHDLNGVDATKVF
                     QDGKIDHQILNAALTLQRQNKDRKVVLVTKDVALRLKAKSLSITSEDYLTGKVRDVRN
                     AMYNGRTVLDEVDETLIDTLFEQGHMPVGEMGLEKPAGNHFFILKHKKKSILSKYDPR
                     TGLVNRVEKRSVFGIGPKNAEQTLAMAALLDPEIKLVTMQGAAGTGKTLLALACALEQ
                     RRDYRQIYVTRPVVPLSNKDIGYLPGDIQSKLDPYMQPLWDNLKLIKGSFEKHDSTPK
                     RIDEMLENEKIAIAPLAYIRGRSLSNIFFIVDEAQNLTPLEVKTV"
BASE COUNT          323 a          405 c          388 g          227 t
ORIGIN      
        1 ctagtcatgg agggacatgg ctgatccgcc tgcgctggtg ctcgagcggt tgaaaaggcg
       61 cgcgatcgca ggatcgcgag tgatagcgcg gttcttgccc gttccgagcg cacgatcaaa
      121 agatcgatcg ttcaaaagtg cgtgcgtcgg ggctggcgct tggatccaca tgtccacact
      181 tttgggcaac actgttcctt gatcggatga agaaaaaaga ccggaagatc ttcgtgctgg
      241 atacatcggt catcctgtac gaccactcgg ccatctcctg cttccaggag cacgacgtgg
      301 ccatccccat acaagtgctg gaggaactcg acacgttcaa gaaagggaat gataccatca
      361 acttcgaggc gcgcgagttc atccgccacc tcgatggcat cgccaaaggc gacctgctca
      421 cggactggac cccgttgaac ggctccacaa cgggcaagtt caaggtggtg gccaagcacg
      481 acctcaatgg cgtggacgcc accaaggtgt tccaggatgg caagatcgat caccagatcc
      541 tgaacgccgc cctgacgctg caacgacaga acaaggacag gaaggtcgtg ctggtgacca
      601 aggacgtggc cctgcgcctg aaggccaaaa gcctcagcat caccagcgag gactacctca
      661 ccggcaaggt gcgcgatgta cgcaacgcca tgtacaacgg ccgcacagtg ctcgatgagg
      721 tcgatgaaac gctcatcgat acgcttttcg agcagggaca tatgccggtc ggggaaatgg
      781 ggctggagaa gccggccggt aaccacttct tcatccttaa acacaagaag aagagcatcc
      841 tcagcaagta cgacccgcgc accggcctgg tgaatcgcgt ggagaaacgt tccgttttcg
      901 gcatcggtcc gaagaacgcc gaacagaccc tggccatggc cgccctgctc gatccggaga
      961 tcaagctggt gaccatgcag ggggcggccg gtacgggcaa gaccctgctg gcactggcct
     1021 gtgccctgga gcaacgccgc gactaccggc agatctatgt tacccgaccc gtggtaccgc
     1081 tgagcaacaa ggacatcggc tacctgcccg gcgatatcca gagcaagctg gatccctaca
     1141 tgcaaccact ctgggacaac ctcaaactca tcaagggttc gttcgagaag cacgacagca
     1201 cgcccaagcg catcgacgag atgctcgaga acgagaagat cgccatcgca ccgctggcct
     1261 acatccgtgg ccgcagcctg agcaacatct tcttcatcgt tgacgaggcc cagaacctca
     1321 cgccgctgga ggtgaagacc gtg
//
LOCUS       DAOQOC010001011         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_86619_length_1342_cov_2.450660, whole genome shotgun sequence.
ACCESSION   DAOQOC010001011 DAOQOC010000000
VERSION     DAOQOC010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86619_length_1342_cov_2.450660"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PL106_13915"
     CDS             <1..250
                     /locus_tag="PL106_13915"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNA34219.1"
                     /translation="MKRHLEWGGESGLTTMPRRELFRLAARYSLIEDPQRWFEYHEGR
                     NLTSHTYDEANARKVVALLKGFVKDTAYLLNTLKEHHA"
     gene            243..533
                     /locus_tag="PL106_13920"
     CDS             243..533
                     /locus_tag="PL106_13920"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNA34220.1"
                     /translation="MLDLPADQLALVRAILARHVPHAKVWAFGSRVQGTAERFSDLDL
                     AIEDTEELSLRTLGNLKHDFIESDLPISVDILDMRTVKQPFKGIVVRQRVAL"
     gene            complement(594..>1342)
                     /locus_tag="PL106_13925"
     CDS             complement(594..>1342)
                     /locus_tag="PL106_13925"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34221.1"
                     /translation="EIEGQKARIEGLLLNILPKSISDELNEKGRATARMHPEVTVMFT
                     DMKGFTRVAEKMTPEELVRELDECFIQIDQIIGRYGIEKIKTIGDSYMCASGIPQADP
                     WHAHKMILAAIEVREMMARWCRQREALGKEPWMLRIGIHTGPVVAGVVGQRKFAYDIW
                     GDTVNTASRMESSGATGELNISGRTYEVVKDDFVCEYRGKVEAKSKGQIDMYFVRRID
                     PELSADANGVWAAPGYLRSLGLTGMTGTLG"
BASE COUNT          238 a          433 c          401 g          270 t
ORIGIN      
        1 gatgaagcgg catttggaat ggggcggtga aagcggtctg accacgatgc cccggcgtga
       61 actgttccgc ctggccgcac gctattcgct cattgaggac ccacaacgct ggttcgaata
      121 ccacgaaggc cggaacctca cgagccacac gtacgatgag gccaacgcac gcaaggtggt
      181 agccttgctg aagggcttcg tgaaggacac agcctacctg ctcaacacat tgaaggaaca
      241 tcatgcttga cctgcccgct gaccagctcg ccttggtgcg cgccatcctg gcccggcatg
      301 taccccacgc caaggtgtgg gccttcggca gccgggttca aggcaccgcg gaacgcttca
      361 gcgatctgga cctcgccatc gaggacacgg aggaactttc cctacgcacc ttggggaacc
      421 tgaagcacga cttcatcgag agtgacctgc ccatcagcgt ggacatcctg gacatgcgta
      481 cggtgaagca gcccttcaag ggcatcgtgg tgcggcagcg agtggcgttg tgagtgtgga
      541 ggaccgcgtc gtgggcggtc cgaggaatta aggcaaggcc agaaggctcc cgtctacccc
      601 agcgtcccgg tcatgccggt cagtcccagc gatcgcaggt agccgggtgc ggcccacaca
      661 ccgttggcat cggcgctcag ttcggggtcg atcctgcgaa cgaagtacat atcgatctgc
      721 cccttgctct tggcttccac cttaccgcgg tactcgcaca cgaaatcgtc cttgaccacc
      781 tcgtacgtgc ggccgctgat gttcaactcc ccggtcgccc cgctgctctc catgcggctg
      841 gcggtgttca ccgtgtcgcc ccagatatca taggcgaact tgcgctggcc caccaccccg
      901 gccaccacgg gcccggtgtg gatcccgatc cgcaacatcc agggttcctt gcccagggct
      961 tcgcgctggc ggcaccagcg ggccatcatc tcgcgcacct cgatggcggc gaggatcatt
     1021 ttgtgcgcgt gccaggggtc ggcctgggga atgccactgg cgcacatgta gctgtcgccg
     1081 atggtcttga tcttctcgat gccgtatcga ccgatgatct ggtcgatctg gatgaagcac
     1141 tcgtccagtt cgcgcacgag ttcctccggg gtcatcttct cggccacgcg ggtgaaaccc
     1201 ttcatatcgg tgaacatcac ggtgacctcg gggtgcatgc gcgcggtggc ccttcccttc
     1261 tcgttgagtt cgtcgctgat actcttgggc aggatattga gcagcagtcc ctcaatgcgc
     1321 gctttctgcc cttcgatctc ct
//
LOCUS       DAOQOC010001012         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_86699_length_1341_cov_3.437792, whole genome shotgun sequence.
ACCESSION   DAOQOC010001012 DAOQOC010000000
VERSION     DAOQOC010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86699_length_1341_cov_3.437792"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PL106_13930"
     CDS             complement(<1..709)
                     /locus_tag="PL106_13930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA34222.1"
                     /translation="MHIESKGTDPFEQRLKDSLGQFEVPYNSADWTQLENALDGNKRG
                     WWGTRARVSAVIAGLALLAGTAWYTVNRPEVPESMTAEAQRSGSTTNIEPMGPEGEKP
                     ATVSETGSGHLAAVEPEGDLASSKADNADQQAGKGVKNSPEEPQRTAVASTSASSRST
                     QAAPFSTKEAPSPSSDAAFHSSVAEGCPGTSVEFKVANMPADGIYLWNFGDGNFSNKA
                     NPEHTFTKPGTYQVMLSM"
     gene            complement(713..>1341)
                     /locus_tag="PL106_13935"
     CDS             complement(713..>1341)
                     /locus_tag="PL106_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA34223.1"
                     /translation="LATLDHPARLGGTWQGVNSSEGLTGIIDGCIAGERRCQQRVYEL
                     YYGKMMTVCLRYTKNTDQAKDILQDGFIKVFRSMEKFNRDGSFEGWIRRIMVNTAIDY
                     FRRAKNAYLLLGEERSIEDFGDQDEEDTLVDDAEDELLDLRPADVINAMQKLTPAYRT
                     VFNLYVFEEMTHKEISEMLGINIGTSKSNFAKAKHNLKKLLKKEHKLP"
BASE COUNT          221 a          394 c          390 g          336 t
ORIGIN      
        1 tcatcgagag catcacctgg tacgtacccg gtttggtgaa ggtgtgttcc ggattggcct
       61 tgttgctgaa gttgccatcg ccgaagttcc acaggtagat cccgtcggcg ggcatattgg
      121 ccactttgaa ttcgacggag gtaccagggc agccttcggc cacgctggaa tggaaagcgg
      181 catcgctgga tggggagggt gcttccttgg tgctgaaagg ggcagcctgc gtggaacgcg
      241 agcttgcgga agtggacgca acggccgttc tctgcggctc ttccggtgaa ttcttcaccc
      301 ctttgccggc ttgttgatcg gcgttatcgg ctttggagga ggccaggtcg ccttcgggct
      361 caaccgcagc aaggtgtccc gagcctgttt cagacacggt tgcgggtttt tcgccttcag
      421 ggcccatcgg ctcgatgttg gtcgtgcttc cgctccgttg cgcttccgcg gtcatgcttt
      481 cgggaacctc cggtcggttc acggtgtacc acgcggtccc ggccagcagt gccagaccgg
      541 cgatcaccgc cgatacgcgt gcacgggtgc cccaccaacc tcgcttgttg ccgtccaacg
      601 cgttttccaa ctgggtccag tcagcgctgt tgtacggcac ttcgaactgg ccgagactgt
      661 ccttcagtcg ctgctcgaac ggatccgtgc ctttgctttc aatatgcata tctcaaggga
      721 gtttgtgctc ctttttaagc agtttcttca agttgtgttt cgctttggcg aaattgctct
      781 tgctcgttcc tatgttgata cccagcatct cgctgatctc cttgtgggtc atctcttcga
      841 agacatacaa gttgaacacg gtcctgtacg cgggcgtgag cttctgcatc gcattgatca
      901 cgtcggcggg ccgaaggtcc agcagttcgt cctccgcatc gtccaccagg gtgtcctcct
      961 cgtcctgatc gccgaaatcc tcgatgctgc gttcctcgcc caagagcagg taggcgttct
     1021 tggcacggcg gaagtaatcg atggccgtgt tcaccatgat ccggcggatc caaccctcga
     1081 agctgccgtc gcgattgaac ttctccatgg accggaacac cttgatgaag ccgtcctgca
     1141 ggatgtcctt ggcctgatcc gtgttcttcg tgtaccgaag acaaacggtc atcattttgc
     1201 cgtaatacag ctcgtatacc cgctgttggc agcgacgctc tccggcgatg cagccgtcga
     1261 tgatccccgt aagcccttcg gaggagttca cgccctgcca tgttccgcct agacgggcag
     1321 ggtggtcgag ggttgccaat t
//
LOCUS       DAOQOC010001013         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_86718_length_1341_cov_2.976672, whole genome shotgun sequence.
ACCESSION   DAOQOC010001013 DAOQOC010000000
VERSION     DAOQOC010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86718_length_1341_cov_2.976672"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PL106_13940"
     CDS             complement(<1..617)
                     /locus_tag="PL106_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNA34224.1"
                     /translation="MPNDMDPADIRIVVIGVGAIGSALLPRLLRMPFRVVTLVDGDRV
                     EARNLERQELYAPVDVGSPKVQVAGSWARNAPVGPRVEVHDTFLDDRNAESIIAVHDI
                     VADCTDDAHARRLIDRVCGDLGITLVSGAVHAGQGQVIVLHDEGPGEDISLEYLFPGR
                     ISMDQDGCDMRHVPLRTIDESAKRMALRIRQVLDHGPVVNGRIDL"
     gene            complement(710..>1341)
                     /locus_tag="PL106_13945"
     CDS             complement(710..>1341)
                     /locus_tag="PL106_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34225.1"
                     /translation="KVYADGAGTRTIQLRDMSDNSTVAEGTFNIPDGESRVDLNFTVP
                     AAGNYGLRYSSQNPGCWRDGQDGSIPYPFALGSLGSITGTNVTGGNALAYYYYFYDWE
                     VQSPLAVCESARTEVVVEVGPQGINDGTAQGFSVYPVPTDAALTIDFGTIQGEVDLDL
                     LDVTGRTVIAQHRTVNGPGQLDLGGLARGEYSLRVRHTGGQLVRRVVVR"
BASE COUNT          245 a          444 c          408 g          244 t
ORIGIN      
        1 aacaggtcga tccggccgtt cactacgggc ccgtgatcca gtacttgccg gatgcgtaaa
       61 gccatgcgtt tggcggactc gtcgatcgtc cgcaagggga catggcgcat atcgcacccg
      121 tcctgatcca tgctgatcct tcccgggaac aggtattcga gggagatgtc ctccccgggt
      181 ccttcgtcgt gcaggacgat cacctggccc tgccccgcat ggaccgcccc actcaccagg
      241 gtgatgccca ggtcaccgca aacgcgatcg atgagccgtc gagcatgggc atcgtccgtg
      301 caatcggcca cgatgtcgtg tacagcgatg atggactccg cgttccggtc atccagaaag
      361 gtgtcatgta cttctacccg tggacccact ggagcattgc gcgcccacga ccccgcgacc
      421 tgcactttgg gcgatccgac atccacaggg gcgtacaact cctgccgttc caggttacgc
      481 gcctccaccc ggtcaccatc caccagggta actacgcgga agggcatacg gagcagcctc
      541 ggcaacagtg ccgaaccgat ggcaccgaca ccgatcacga caatgcggat gtccgcaggg
      601 tccatgtcgt tgggcatcgc ggggccaagt tacctcgagc cgtcgttccg aacgggaacg
      661 gccccggccc gaagagttcg gaccggggcc gtaccgtggc catccgtgct cagcggacca
      721 caacgcggcg gaccaactgg ccccccgtat gccgtacccg caggctgtac tcaccacgag
      781 caagaccacc cagatcgagc tggcccggtc cgttcacggt acggtgttga gcgatcaccg
      841 tgcggccggt cacatccagc agatccaggt ccacttcgcc ttggatggtg ccgaagtcga
      901 tggtcagcgc ggcgtctgtg gggacggggt acaccgagaa gccctgtgcc gtgccgtcgt
      961 tgatcccttg cggacctact tccacgacaa cttccgtacg cgcgctttca cacaccgcga
     1021 gcgggctctg cacctcccag tcgtagaagt aatagtagta cgccagcgcg ttgcctcccg
     1081 tgacgttggt accggtgatg gagccgagtg agccaagtgc gaagggatag gggatgctgc
     1141 cgtcctgtcc atcgcgccag cagcccgggt tctggctgga gtagcgcagg ccgtagttgc
     1201 ccgccgctgg tacggtgaag ttgaggtcca cgcggctttc cccatcgggg atgttgaacg
     1261 tgccttccgc caccgtggag ttgtcgctca tgtcgcgcaa ctggatggta cgcgtgccgg
     1321 caccatcggc atacaccttc a
//
LOCUS       DAOQOC010001014         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_86967_length_1339_cov_4.382399, whole genome shotgun sequence.
ACCESSION   DAOQOC010001014 DAOQOC010000000
VERSION     DAOQOC010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86967_length_1339_cov_4.382399"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..>1339
                     /locus_tag="PL106_13950"
     CDS             275..>1339
                     /locus_tag="PL106_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34226.1"
                     /translation="MLRKVDAGWWLAIGFILACCAYEYGRVFGSRPFSHHLWRQTSCL
                     SMTYNLYMGEGGLLEPWFHNFFGDHGTSGRSAGEFTGLYWFVAWIWKLTGPREDVYRA
                     VMLLLHFAGTWSLFQALRKLLSHGGWAALSALFFFGTPVMAYYSVGFLTEVPAFDLVL
                     VGVWAMVRHYPLVRASDVLVACLFFALAGLLKITALMAPLTLFIWLLIEWLCDSSRVG
                     RAVLFPNKPVSLMAFLAAFGIVLAWTLHARNYNNTHGVFFSNQSILPIWTLPADRIVY
                     YWECGRDLLVYQLFDTPGWYVLLGMLVYVVLSWKAALRMLGVPLLFLVIGVLLYSILW
                     WEALDGHDYYYIIPMIVPVVL"
BASE COUNT          214 a          391 c          409 g          325 t
ORIGIN      
        1 gcacggtagc cgccgtggag cggtacatgg ggatcaaccc cgccatgggc atccgatctg
       61 gcgcattcac tgctgaaccc ggggcggcag gttgtcattg ttgaccctcg gaaggaattg
      121 aagaaaaact gcccgacgaa gtacaatact tccgcacgga aggagccgga ttccgtcacc
      181 cccccgatct gaccgcatga accaccggtc cccgaagaaa atgccgatgg acgcggttgg
      241 tggtttgagg cttcattccc ggaggttcct gcgcatgttg cgcaaggtcg atgccggttg
      301 gtggcttgcg atcggcttca tcctcgcgtg ctgcgcttat gagtacggaa gggttttcgg
      361 atcacgtcct ttttcgcacc acctctggcg ccagaccagt tgcttgtcga tgacctacaa
      421 cctgtacatg ggtgagggcg gattgctgga gccctggttc cataattttt tcggcgacca
      481 cgggacatcc gggcgcagtg ccggggagtt caccgggctg tattggttcg tggcctggat
      541 ctggaagctc acaggacccc gagaggacgt gtaccgcgcc gtgatgctcc tgctgcattt
      601 cgcgggtacc tggtccctgt tccaagcact ccggaaactc ctgagccacg gcggatgggc
      661 cgcgttgtcg gcactattct tctttggcac ccccgtgatg gcctactatt ccgtgggttt
      721 ccttaccgag gtgccggcat tcgatctggt actggtgggc gtatgggcca tggtgcggca
      781 ctacccgttg gtgcgcgcca gcgatgtgct ggtcgcctgc ctgttcttcg cgctggccgg
      841 tctgctgaag atcaccgcgt tgatggcgcc tctaaccctg ttcatctggc tgctcatcga
      901 gtggttgtgc gactcctcga gagtggggcg tgcggtgctt ttccccaata agccggtaag
      961 cctgatggcc ttcctggcgg ccttcggcat cgtgctggca tggacccttc atgcccggaa
     1021 ctacaacaac acacatggcg tgttcttttc gaaccagtcc atcctgccga tctggaccct
     1081 gcccgcggac cggatcgtgt actactggga atgcggccgt gacctcctcg tatatcaact
     1141 cttcgacaca ccggggtggt atgtgctgtt gggcatgctc gtctatgtag tgctgagttg
     1201 gaaggcggcc ctgcgcatgc tcggcgtacc gctcctgttc ctggtcatcg gcgttctgct
     1261 gtactccatt ctgtggtggg aggcgctcga tggccatgac tattattaca tcatcccgat
     1321 gatcgtgccg gtcgtgttg
//
LOCUS       DAOQOC010001015         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87289_length_1337_cov_2.996880, whole genome shotgun sequence.
ACCESSION   DAOQOC010001015 DAOQOC010000000
VERSION     DAOQOC010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87289_length_1337_cov_2.996880"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PL106_13955"
     CDS             complement(<1..610)
                     /locus_tag="PL106_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34227.1"
                     /translation="MNANATGSATGLSWTNAFTDLQVALSMVIPGDEIWVAAGQYRPT
                     TGTSRTSSFQLRNGVDLYGGFVGTETLLEERDLEANITVLNGDIGEPGTTTDNSYRVV
                     TANNINTHIVLDGFRIMNGYSAAGNGYNGGGLNVQNALGGNVHVRNCLFVNNYSGSYG
                     GGIYLAAAVVTLEDCTFNNNSAGTGGDGGAIYNGNNNGGFSIL"
     gene            1033..>1337
                     /locus_tag="PL106_13960"
     CDS             1033..>1337
                     /locus_tag="PL106_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34228.1"
                     /translation="MAYRPVAVVAQSGHELGPGRESAVVGAGFALHGFSLWHHQRNKD
                     RPLPPLDLARVPAFDRISTTQWSLRAHRTSNILFGVVATAAFTTAIIAQRDDRPEVPA
                     "
BASE COUNT          246 a          418 c          413 g          260 t
ORIGIN      
        1 caaggatcga gaagccacca ttgttgttgc cgttgtagat ggctccccca tcgccgccgg
       61 ttcctgcgga gttgttgttg aacgtgcaat cctcgagggt cacgaccgcc gcagcgaggt
      121 agatgccccc gccgtatgac ccggaatagt tgttgacgaa caggcaattg cgcacgtgca
      181 cgttgccgcc caacgcgttc tgcacgttca gcccgccacc gttgtaaccg ttgcccgccg
      241 cgctgtaccc gttcatgatc cggaaaccgt cgagcacgat gtgcgtgttg atgttgttcg
      301 ccgtgaccac ccggtatgaa ttgtccgtgg tcgttcccgg ctcgccgatg tcgccgttca
      361 gcacagtgat gttcgcctcg aggtcacgct cctcaaggag ggtctccgtc ccgacgaagc
      421 cgccgtacag atccacgcca ttgcgcaact ggaacgaact ggtacggctg gtgcccgtgg
      481 tgggacggta ttgccccgca gccacccaga tctcgtcccc ggggatcacc atggacagcg
      541 ctacctgcag gtcagtgaag gcgttcgtcc aactcaaccc ggttgcggag ccggtcgcgt
      601 tcgcattcac gtgataacgt gttgcatatg cgctgctcgc aagggagaac aaggtcaaaa
      661 gcaagcacgc gcggacaaga gcactggtca tggccgaatg aggagttggg caagagttgc
      721 cgcgaaagta ccgcgcacat ccatggcgat cgggctgcgt ggtgcgtgat gcacgatgcg
      781 gagacctgaa aagacgcgac cttggcggtc gatcggatgt gtaccgtaca gttccatgcg
      841 cctgctggtc cggccgttcg tcgccccgaa ccgccgggcc tgtcccaagg ggatgcgcgt
      901 tctggtcccg catcagttgc acaagaacgc agcggagaac cgatggatag ttccgagcgg
      961 tcatgggcaa attggtcggc gttcctgaag gtcgcatacc gggggatcat gcttcaagtc
     1021 ctggtacttc tgatggctta tcgtccggtg gcggttgtag cacagtccgg tcacgagctc
     1081 ggccccgggc gcgaatcggc cgttgttggc gcgggcttcg ccctgcacgg gttcagccta
     1141 tggcaccacc agcggaacaa ggatcggccg ctgccaccgc tcgacctggc ccgggttccc
     1201 gccttcgacc ggatcagtac gacgcagtgg agcctgcggg cccaccgaac cagcaacatc
     1261 ctgttcggtg ttgtcgccac cgccgctttc actaccgcca tcatcgccca acgcgatgat
     1321 aggccagaag taccggc
//
LOCUS       DAOQOC010001016         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87400_length_1336_cov_3.519906, whole genome shotgun sequence.
ACCESSION   DAOQOC010001016 DAOQOC010000000
VERSION     DAOQOC010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87400_length_1336_cov_3.519906"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PL106_13965"
     CDS             <1..545
                     /locus_tag="PL106_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNA34229.1"
                     /translation="YPVIDADPEKVVGVVTLKDLFTHLEDPEPALGRILRDPPWLPEG
                     LSAYEALRRFKRTGQRYGLVTDEFGEAQGLVTLNDILEALVGDVGEFHGGEFALTRRE
                     DGSWLVDGHYPLHELLVRLGQPGLARDVEADTIGGLFLYQTGRIPVTGEKLRWMIFDL
                     EVVDMDGVRIDKLLVSPVKD"
     gene            747..>1336
                     /locus_tag="PL106_13970"
     CDS             747..>1336
                     /locus_tag="PL106_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34230.1"
                     /translation="MDIRRYAAFLLLLLASTIVRATHIGGGEIYWDHLGGNQYRITLI
                     VYRDCAGVTLDNGYNLDITSPCGTMQLWVDNQGTTEVSQLCGAQLPNSTCNGGTLPGI
                     QRRVYTGTVNLPPCDSWTISWTEIYRNNAIVNLQTPGSQEVYIEGVLNNAAGPANDSP
                     LFSNTTAVPYVCLGYPVSYSYGAYDPEADSLSYEFI"
BASE COUNT          255 a          411 c          430 g          240 t
ORIGIN      
        1 tgtaccccgt gatcgatgcg gatccggaga aggtggtcgg tgtggtgacc ctgaaggacc
       61 tgttcaccca tctcgaagac ccggagccgg cactggggcg catcctgcgc gacccgccct
      121 ggcttcccga ggggttgagc gcgtacgagg cgctgcggcg attcaagcgg accggtcaac
      181 gctacggtct ggtgaccgat gagttcgggg aggcgcaggg gctggtcacc ttgaatgaca
      241 tcctggaggc gctcgtgggc gacgttggcg agttccatgg cggggaattc gcgttgaccc
      301 ggcgcgagga cgggtcctgg ttggtcgatg gtcattaccc cctgcacgag ctgctcgtac
      361 ggctcgggca gcccggtctt gcccgtgatg tggaagcgga caccatcggc ggcctcttcc
      421 tgtatcaaac cggccggatc cccgtaacag gggaaaaatt gcgctggatg atcttcgacc
      481 tcgaggtcgt ggacatggac ggcgtacgga tagacaagct gctggtgagc ccggtcaagg
      541 actgaggcac ggcccgaacg ggtgctgacg tcccccgcgg cgttccgtca attccccatc
      601 ctgcggcacc tcgaacaagc tatctgtcga gtggatgtaa ccctcggggc ttctttcctg
      661 tttcatacgt cgagaacacc cgccatcccg gtgcgccgac aggacgaaag tccccggaac
      721 cgtgaggcgg gagaacaccc gggccgatgg acatccgacg ttacgcagcc ttcctgctcc
      781 tcctcctggc cagcaccatc gttcgcgcca cccacatcgg tggtggagag atctactggg
      841 accacctcgg tggaaaccaa taccggataa cgctgattgt ttaccgcgat tgcgccgggg
      901 tgaccctgga caacgggtac aacctggaca tcaccagccc gtgcggcacc atgcagttgt
      961 gggtggacaa ccagggaacg accgaggtct cgcagctctg tggtgcgcaa ctgcccaaca
     1021 gcacatgcaa cggcgggacc ctgcccggga tacagcggcg cgtgtacacc gggacggtta
     1081 acctcccgcc ctgcgacagc tggacgatca gctggaccga gatctaccgg aacaatgcca
     1141 tcgtgaatct gcaaaccccg ggatcgcagg aggtgtacat cgaaggggtg ctgaacaatg
     1201 cggccggccc ggcgaacgac tcgccgctgt tctccaatac aacggctgtt ccttacgtgt
     1261 gtctggggta cccggtgagc tacagctatg gcgcgtacga ccccgaggcg gactcgttga
     1321 gctacgagtt catcga
//
LOCUS       DAOQOC010001017         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87528_length_1335_cov_3.837500, whole genome shotgun sequence.
ACCESSION   DAOQOC010001017 DAOQOC010000000
VERSION     DAOQOC010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87528_length_1335_cov_3.837500"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PL106_13975"
     CDS             complement(<1..560)
                     /locus_tag="PL106_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNA34231.1"
                     /translation="MAFCVLTAHATCAQDVHFSQFFNAPLTLNPALTGAITSDQRAAL
                     IHRSQWQSLGAPFRTFAFSYDAPLLRGRMNGRYLGVGATAYSDKAGKSGYGDTQGGIS
                     LAYGLRTGDAGILAAGIQCAYGQRSAVLDGLRWDAQYDGSGYDPSRPTGESLPNTSTS
                     FIDLSSGVAWQHRDGEDIQWSAGIAA"
     gene            complement(617..>1335)
                     /locus_tag="PL106_13980"
     CDS             complement(617..>1335)
                     /locus_tag="PL106_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA34232.1"
                     /translation="GEPGHVTGTVAEPGTYSFTWNGDAGTEEVPYPAPAGELITVHVE
                     NEGTGCGVDTLLRIPGWPALTALFSPNPNEPCVPFEQSLITFIDLSNNAIGGTWTIGS
                     MTTPYVPGENPDYDHGQAGSYPVQLVVYNIGGCTDSFALEVCIAPSTAIFIPDIFSPN
                     GDGNNDRLFVRGGGIASMEFRVFDRWGGMVFHCSDNATGWDGTTGNGPAPSGVYVYTF
                     SATMLSGKTLEMNGDLTLVR"
BASE COUNT          228 a          405 c          461 g          241 t
ORIGIN      
        1 aaggcggcga tgcccgcgct ccactggatg tcctccccgt cccggtgctg ccaagcgacc
       61 ccggaggaca ggtcgatgaa tgaggtcgaa gtgttgggga ggctctcgcc agtgggccgc
      121 gaggggtcgt acccgctgcc gtcgtactgg gcatcccagc gcagaccatc gagtaccgca
      181 ctgcgctggc cgtaggcgca ctgtatgccg gcggcaagga tgcccgcatc accggtgcgc
      241 aggccgtacg ccagactgat gccgccttgt gtatcgccgt agccgctctt gccggccttg
      301 tcgctgtacg cggtcgcacc gacgccgagg tagcgcccat tcatccggcc gcgcagcagc
      361 ggagcgtcgt agctgaaggc gaacgtgcgg aacggggcac caaggctctg ccattgggag
      421 cggtggatca gggcggcccg ctgatcggat gtgatcgccc cggtgagcgc ggggttcaac
      481 gtgagcggag cgttgaagaa ctgggagaag tggacatcct gggcacaggt cgcatgggct
      541 gtgagcacgc agaaggccat tgcgggtacg tgccgtcgac cacggcacag ggacagtgat
      601 agggcacgag gtgcgttcat cggacgagtg tcagatcacc gttcatctcc agggttttgc
      661 cgctgagcat ggtggctgag aacgtgtaca cgtatacccc ggaaggcgcc ggtccgttac
      721 ccgttgtacc gtcccagccg gtagcgttat cgctgcaatg gaagaccatt ccgccccagc
      781 ggtcgaacac acggaactcc atgctggcga tgccgccgcc acgcacgaac agccgatcgt
      841 tgttgccgtc gccgttgggg ctgaagatgt cggggatgaa gatcgccgtg ctcggggcga
      901 tgcacacttc cagggcgaac gaatcggtgc atccgccgat gttgtacacg accagttgca
      961 ccggatacga gccggcctgg ccatggtcat agtccgggtt ctcgccgggg acgtacggtg
     1021 tggtcatgct cccgatggtc caggtgccgc cgatggcgtt gttgctcagg tcgatgaagg
     1081 tgatcaggct ctgctcgaag ggcacgcagg gctcgttggg gttcggactg aacagggcgg
     1141 tgagtgcggg ccagcccggg atccgcaaca gcgtgtccac gccgcagccg gtgccttcgt
     1201 tctccacgtg tacggtgatc aattcgcccg caggagcggg atagggcacc tcctccgtac
     1261 ccgcatcacc gttccatgtg aagctgtagg tgccgggttc ggccaccgtt cctgtcacat
     1321 gcccgggttc gccat
//
LOCUS       DAOQOC010001018         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87539_length_1335_cov_3.492188, whole genome shotgun sequence.
ACCESSION   DAOQOC010001018 DAOQOC010000000
VERSION     DAOQOC010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87539_length_1335_cov_3.492188"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PL106_13985"
     CDS             complement(<1..253)
                     /locus_tag="PL106_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34233.1"
                     /translation="MSLPPAQCPRTGRVLRCSCLAPARIASGSGVCSFVAASSPSALL
                     GRCFGKLPLRPLTRCDRSCDPQHRPPDNLRLGNQGRLARG"
     gene            386..>1335
                     /locus_tag="PL106_13990"
     CDS             386..>1335
                     /locus_tag="PL106_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA34234.1"
                     /translation="MWITRYAVKNSPFTLVIFLMIVVVGISTLLGMPRAEDPEMNAPI
                     YPIVAVYPGTSPEDMEKLVVDPIEKRVAELDDIKRVYATIKDGVAVIRVDALYGVDVD
                     RKHQELVQEINALRAELPADIHSMEVQKATPSSVNVLQVALVSENAPRRALKEQAEHL
                     QEALEALPMLKKVEVNGLPGATVRIDLQLEKLARLRIPLAQVVGAVQSEMAHIPGGSV
                     DAGTKALNVKTSGSVVDAADLARIVVATADGRNTLLRDVAQVTDAYHEDRHITRLNGH
                     RSVVVNAALKSGMNISTAQQAYLPVLEKFRAQLPENIALE"
BASE COUNT          267 a          398 c          481 g          189 t
ORIGIN      
        1 ccccacgggc aagccgtccc tggttgccca gccggaggtt gtcgggtgga cgatgttgcg
       61 gatcgcagga acgatcgcac cgcgtgagcg ggcgcagcgg cagcttgccg aagcagcggc
      121 ccagcagcgc actcggcgag gaggctgcga cgaaggagca gaccccggag ccggatgcga
      181 tgcgggccgg agcgaggcaa ctacagcgga gcacgcggcc cgtgcgtggg cattgcgcgg
      241 ggggaaggga cacgccccaa taccgaatac ggtgaatggc gaacgggagt tggtgaatgg
      301 tacgccacgc ttgtcccatt cgctattcac catccaccat tcgccgatca cccattccca
      361 ttcaccgcat tagcgcattc gacccatgtg gatcacccga tacgccgtta agaacagtcc
      421 gttcacgctg gtcatcttct tgatgatcgt ggtggtgggc atcagcacgc tgctgggcat
      481 gccgcgcgcg gaggacccgg agatgaacgc gccgatctat ccgatcgtgg cggtttaccc
      541 gggcaccagc cccgaggaca tggagaagct ggtggtggac ccgatcgaga agcgcgtggc
      601 cgagctggac gacatcaagc gggtgtatgc caccatcaag gacggcgtgg cggtgatccg
      661 cgtggatgcg ctttacggtg tggacgtgga ccgcaagcac caggagctgg tgcaggagat
      721 caacgcgctg cgcgccgagc tgcccgccga catccacagc atggaggtgc agaaggccac
      781 gcccagcagc gtgaacgtgc tgcaggtggc gctggtgagc gagaacgccc cgcgccgtgc
      841 cctgaaggaa caggctgagc acctgcagga ggcgctggaa gccctgccga tgctgaagaa
      901 ggtggaggtg aacggactgc ccggcgcaac ggtacggatc gacctgcagt tggagaagct
      961 ggcgcggttg cggatcccgc tggcccaagt ggtcggcgcg gtgcagagcg agatggccca
     1021 catacccggc ggcagcgtgg atgcaggcac gaaggcgctg aacgtgaaga ccagcggcag
     1081 cgtggtggat gcggccgatc tggcacgcat cgtggtggcc acggccgatg gccgcaacac
     1141 cctgctgcgc gatgtggcgc aggtgaccga cgcgtaccac gaggacaggc acatcacgcg
     1201 gctgaacggc caccgcagcg tggtggtgaa tgcggcgctg aagagcggca tgaacatcag
     1261 cacggcgcag caggcatacc tgccggtgct ggagaagttc cgcgcccagt tgccggagaa
     1321 catcgcactg gagca
//
LOCUS       DAOQOC010001019         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87554_length_1335_cov_3.221875, whole genome shotgun sequence.
ACCESSION   DAOQOC010001019 DAOQOC010000000
VERSION     DAOQOC010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87554_length_1335_cov_3.221875"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..724
                     /locus_tag="PL106_13995"
     CDS             197..724
                     /locus_tag="PL106_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34235.1"
                     /translation="MFRSPQHPLRISALTAILVCASITVRAEDPPASRSRIHAPGAVE
                     LTGWLHVEAFDFNDASVLIIVNGERRTVPVSRSGRIDVDLPAEAEAVLRFEHPGHLSK
                     EVLVDTRHAQVGSPAAARHVSFAVILEESRLMGGQVYAGPVANFGFDKDGGCVDVKHT
                     RSLVVGRAVNAPMVF"
     gene            complement(1097..>1335)
                     /gene="tssD"
                     /locus_tag="PL106_14000"
     CDS             complement(1097..>1335)
                     /gene="tssD"
                     /locus_tag="PL106_14000"
                     /inference="COORDINATES: protein motif:HMM:NF037603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HNA34236.1"
                     /translation="YILENKVAPTCKLVLRGGKEDSAVKTIEFTDCYVHDMTESFSSV
                     GGQPATIQFSITCAKMNEDGAKVENEWDALAQVR"
BASE COUNT          240 a          435 c          382 g          278 t
ORIGIN      
        1 ctccaggggg gcaataaagc gacgtcccga tccctccaag cctgttcgcg tgccgagcgg
       61 gaagaagtgg tcttcttagt tcgccgggtc atttcgggcc cttgaccgga tttccagcca
      121 tgcggtcgaa tggaccgcgg atcggggcaa catcaacagc acgacaccgt tccaagcact
      181 gatcacaacc tgcaacatgt tccgatcacc acaacatcct ctgcgcatca gcgcgctcac
      241 cgccatcctt gtctgtgcat ccattacggt ccgtgccgag gatccgcccg caagccgctc
      301 ccgcatccac gcgccgggtg cggtggaatt gaccggctgg ctccacgtgg aagcgttcga
      361 cttcaacgat gcctcggtgc tcatcatcgt gaatggtgaa cgccgaacgg tcccggtttc
      421 ccgttcaggc cggatcgatg tcgatctgcc tgctgaggca gaagccgttc tgcgttttga
      481 acatcccggc cacctgagca aggaggtgct cgtggacaca cgtcatgcgc aagtgggctc
      541 gccagccgct gcccgtcacg tcagcttcgc cgtgatcctg gaggaatcgc gcctgatggg
      601 cggccaggtg tacgccggcc ccgtggccaa cttcggcttt gacaaggacg gtggctgcgt
      661 ggatgtgaaa catacacgtt cgctcgtagt gggccgcgcg gtcaacgcgc ccatggtgtt
      721 ctgatcggct tccgaccgtg ccagccccgg acttgcgggt cgctatcagc ggctcacagg
      781 atccggggct gcgtgcgttc tcgctggtcc atcctccagc ccgatcgttc ggcacatcca
      841 tcgcgcggca tcaataggac tccgtccatc tgcccgtagg cgacccaaag acacatcacg
      901 gtgaaaccgc acgaaccgtc tcgatgggtt cgctgtgaca tgccgaggat agaggacggc
      961 ggtccattgg cgctgaaggt gccctgcccc aaagcagaac cccaccttcc ctcggtcggg
     1021 cggtggggtt cactcgcgtg gtcctgtagc tccctgtgac gaggtctgac aacgcccttg
     1081 ggtccttaac tgcggctcat ctgacctggg ccagcgcatc ccactcattc tccaccttgg
     1141 cgccatcttc gttcatcttc gcacaggtga tgctgaattg gatggtagcc ggctgaccgc
     1201 ctaccgagct gaacgattcg gtcatgtcat gcacgtaaca atcggtgaac tcgatcgttt
     1261 tcactgcgga atcctccttg ccgccccgta gtaccaactt gcaggtagga gccaccttgt
     1321 tctccagaat gtact
//
LOCUS       DAOQOC010001020         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87561_length_1335_cov_3.106250, whole genome shotgun sequence.
ACCESSION   DAOQOC010001020 DAOQOC010000000
VERSION     DAOQOC010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87561_length_1335_cov_3.106250"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PL106_14005"
     CDS             <1..653
                     /locus_tag="PL106_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34237.1"
                     /translation="GDHVVELCYEKPWAPWLMALSMVSFFAVLFVLAFALRGSARTLA
                     LLGSASLMGLVAWSLLVHRPGYDDALRQHRLAERCAPSPSDLEHFKLAGTPLVLAQPE
                     HLYTPAYRTSSDSLLDMDHRALMVDLAFRPHGRCSGKLILQRSYRGRTTDYEAVPFIS
                     PDSSCTRPCTFTLVRDLRELRYRGEELSIYAWNLGTDTITITDLQVRTSPRAVWEE"
     gene            702..774
                     /locus_tag="PL106_14010"
     tRNA            702..774
                     /locus_tag="PL106_14010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:735..737,aa:Gly,seq:gcc)
     gene            850..934
                     /locus_tag="PL106_14015"
     tRNA            850..934
                     /locus_tag="PL106_14015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:883..885,aa:Leu,seq:taa)
     gene            938..>1335
                     /locus_tag="PL106_14020"
     CDS             938..>1335
                     /locus_tag="PL106_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNA34238.1"
                     /translation="MVGIRTRIAPTPSGYLHVGNGLNFLLAHAIARESGGTVLLRIDD
                     LDAERTRPAYVEDIFESLHWLGITWDEGPRDAVDFARNWSQTGRMGPMHNMLERLRDM
                     GVLFACICSRKELAGCTCRDQGLSFDATGTT"
BASE COUNT          233 a          418 c          413 g          271 t
ORIGIN      
        1 gtggcgacca cgtggtggag ttgtgctatg aaaagccttg ggcaccctgg ctgatggccc
       61 tctccatggt cagcttcttt gcggtgctct tcgtgctggc cttcgcgctg cgtgggtcgg
      121 cacgaaccct ggccctcttg ggttcggctt cgctgatggg cctggtggcg tggtcattgc
      181 tggtgcatcg gccgggatac gatgacgcac tgcgccaaca ccggctggcg gaacgctgcg
      241 ccccttcccc aagtgatctc gaacacttca agctcgctgg aaccccactg gtcctcgccc
      301 agcctgagca tctgtacaca ccagcgtatc ggacctcatc cgacagcctg ctggatatgg
      361 atcatcgtgc gctcatggtg gatcttgcgt tccgaccgca tggccgctgc tcgggcaagc
      421 tcatcctgca acggtcctac aggggccgaa caaccgacta tgaggccgtg cccttcatct
      481 cacccgacag cagctgcacg cgcccctgca cattcacctt ggtgcgcgac ctgcgcgaac
      541 tacgctaccg cggcgaggag ttgtcgatct atgcgtggaa cctcggaacg gacacgatca
      601 cgatcacgga tctgcaggtg cgcaccagcc cacgggccgt atgggaagaa tgatggctgg
      661 tatcgcggat tcgatccaca cagctatatt tgcgccccat tgcgagagta gctcagttgg
      721 tagagcacga ccttgccaag gtcggggtcg cgggttcgag tcccgtttct cgctcaaaga
      781 cgcctccgaa agggggcgtc ggtattttcg gccttcccgc ccgccttggc caaggcttcg
      841 gcgggcgaag cccaggtggt gaaatggtat acacgaggga cttaaaatcc cttggtccgt
      901 aagggccttg cgggttcaag tcccgccctg ggcacccatg gtgggcattc gaacgcgcat
      961 cgcgcccacg cccagcggct acctgcacgt gggtaatggg ctcaattttc tgctggccca
     1021 tgccattgcc cgggaaagcg gtggtaccgt gctcttgcgt atcgacgacc tggatgccga
     1081 acgcacccga ccggcgtacg tggaggacat cttcgagtcg ttgcattggc tcgggatcac
     1141 ctgggacgaa ggcccacgcg atgccgtgga cttcgcgagg aactggtcac agaccgggcg
     1201 catggggccc atgcacaaca tgctggaacg gctccgggac atgggcgtcc tgttcgcgtg
     1261 catctgctcg cgcaaggaac tggccggttg tacgtgtcgc gatcaaggcc tttcattcga
     1321 tgccacgggc accac
//
LOCUS       DAOQOC010001021         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87834_length_1333_cov_3.464006, whole genome shotgun sequence.
ACCESSION   DAOQOC010001021 DAOQOC010000000
VERSION     DAOQOC010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87834_length_1333_cov_3.464006"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PL106_14025"
     CDS             complement(<1..742)
                     /locus_tag="PL106_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNA34239.1"
                     /translation="MSDNEIHALINLIDDPDDAVYTQVRDRIISLGAPAVAALERAWE
                     TDDLGDLFRNRIEDILHTIHLDVTYQRLKTWAAGETQDLLEGALIVARYRYPELDEHR
                     VRSKLAALRQDIWLELNDELTPFEKVRVFNHVFFQAHGFKGNKRNYHAPQNSYINEVL
                     DSKQGNPLSLSIIYQVIASELGLPLRGVNLPNHFVLAWTDTGGVSPGLLGHPGVLFYV
                     NTFSQGDILGKNEILGFLISLADGDTSVA"
     gene            990..>1333
                     /locus_tag="PL106_14030"
     CDS             990..>1333
                     /locus_tag="PL106_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HNA34240.1"
                     /translation="MGKPAARIGDMHVCPMVTPGTPPIPHVGGPVTGPGCPTVMIGGM
                     PAATMGDMCTCVGPPDSIILGSTGVFIGGKPAARMGDMCAHGGSIVVGMPTVMIGEVS
                     PGLQAKLLRFLE"
BASE COUNT          235 a          386 c          449 g          263 t
ORIGIN      
        1 acgcaacgct ggtgtcgccg tcggccaggg aaatcaggaa tccaaggatc tcgttcttgc
       61 cgagaatgtc gccctggctg aatgtgttca cgtagaacag tacgccggga tggcccaaca
      121 ggccgggact cactccgccg gtgtccgtcc aggccagcac gaaatggttg ggcaggttca
      181 ctccacgcag gggaaggccg agctcgctgg cgatcacctg gtaaataatg gacagggaga
      241 gcggattgcc ctgcttgctg tccagcacct cgttgatgta cgagttctgc ggcgcgtggt
      301 agttgcgctt gttgcccttg aatccgtggg cctggaagaa gacatggttg aagacgcgta
      361 ccttctcgaa gggagtgagc tcatcgttca actccagcca gatgtcctgc cgcagggccg
      421 ccagtttgct gcgcacgcgg tgttcgtcca actcggggta gcggtagcgg gccacgatca
      481 gggcaccttc cagcaggtcc tgtgtctccc cggcggccca cgtcttcaag cgctggtagg
      541 tgacgtccag gtggatcgta tgcaggatgt cctcgatgcg gttgcggaac aggtcgccca
      601 gatcgtcggt ctcccacgcg cgctccaagg cggccacggc cggtgctccc agtgaaatga
      661 tgcgatcgcg cacctgggtg tacacggcgt cgtccgggtc gtcgatcagg ttgatcagcg
      721 cgtggatctc gttgtcgctc atcgggctgc tcagggcgaa tgtagccagg cgactgggcg
      781 gggcacaagg aggccagtcg gttttccacc gctcagatgt ggatcgcggg gtgggttgac
      841 caagggggat ccttagcaag gctcgtgctt tgttctgccg ggcatggcaa ccggtgccca
      901 tacctgtccc gcttgaaagt tgtgaacaga ccatggatcc aggtaccatt cgggccggga
      961 tcgactacat tagcaccaat actcttggca tgggcaaacc agcagcacgt atcggcgata
     1021 tgcatgtgtg cccgatggtg acaccgggta caccgcccat accgcatgtg ggcgggccag
     1081 tgaccgggcc gggctgcccc acggtgatga tcggcggcat gcccgccgct accatgggcg
     1141 acatgtgtac ctgcgtgggc ccgccggaca gcatcatcct gggctcaact ggcgtgttca
     1201 tcggtggcaa gccggcggca cgcatgggcg atatgtgcgc gcatgggggc tcgatcgtgg
     1261 tgggtatgcc tacggtgatg atcggggagg tgagcccggg tctccaggcc aagctcctcc
     1321 gcttcctcga gga
//
LOCUS       DAOQOC010001022         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_87983_length_1332_cov_3.069695, whole genome shotgun sequence.
ACCESSION   DAOQOC010001022 DAOQOC010000000
VERSION     DAOQOC010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87983_length_1332_cov_3.069695"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PL106_14035"
     CDS             <1..183
                     /locus_tag="PL106_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA34241.1"
                     /translation="WLFYYRAMKEGNVSVVATIDKASIVITLLLSFWILKEPFTWRLA
                     AGATLILAGLVVLIWK"
     gene            complement(197..943)
                     /locus_tag="PL106_14040"
     CDS             complement(197..943)
                     /locus_tag="PL106_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34242.1"
                     /translation="MKKLPEITLLFWCLKICATTLGETAGDLLSMTLNIGYAASSIVL
                     FGAFLIALIAQLSVKHYVPPIYWAVILATSTVGTTLSDYMDRTLGLGYATGSLILASL
                     LGCTLLIWRKVEGSLSVTDIRARRNELFYWAAILCSNTLGTALGDYLADSSGLGFTGG
                     AVLIASILLVDVLAHYFTRIPSVFLFWIAFVLTRPFGATFGDMLTKSQEKGGLDLGTQ
                     GSSLVLFTLLVVLVIVAWRRTHTAPSTIAT"
     gene            complement(1024..>1332)
                     /locus_tag="PL106_14045"
     CDS             complement(1024..>1332)
                     /locus_tag="PL106_14045"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNA34243.1"
                     /translation="NFTQGGTKWSAKYAADAKWLETEHAVKLDELPPAVRTAIVTSYA
                     DHKVEVAEVAESPQGTTYEVDLEKGEHSMEVVFAADGKVLKSVVEEEDEKDEGPDEKD
                     "
BASE COUNT          258 a          395 c          408 g          271 t
ORIGIN      
        1 tggctgttct actaccgcgc aatgaaggaa ggcaacgtga gcgtggtggc cacgatcgac
       61 aaggcgagca tcgtgatcac gctgctgctg tcgttctgga tcctaaaaga gcccttcacg
      121 tggcggctgg cggcaggtgc cacgctgatc ctggcgggtt tggtggtgct gatctggaaa
      181 tagcacagcg tgcacttcat gttgcaatgg tcgaaggtgc cgtatgggtc ctccgccaag
      241 cgacgatcac cagaaccact agtagcgtga acagcaccag cgatgacccc tgcgttccca
      301 gatccagtcc gcctttctct tgcgacttgg tgagcatatc gccgaaggtg gcaccgaacg
      361 ggcgggtgag cacgaaggcg atccagaaga ggaagacgct cggtatgcgc gtgaagtagt
      421 gcgccaacac atcaacgagc aggatgctgg cgatcaacac cgctccgccc gtgaagccca
      481 ggccggaact gtcagcgagg taatcgccca aggcggtgcc caacgtgttg gagcagagga
      541 tggcggccca atagaacagt tcgttgcggc gcgcgcggat gtcggttacg gacaaggatc
      601 cttccacttt gcgccagatg agcagcgtac atcctaacag cgatgcgagg atgaggctgc
      661 cggtggcgta gcccaagccc aaggtccgat ccatgtaatc cgatagcgtg gtgcccaccg
      721 tgctcgtggc caggatcacg gcccaataga tgggcggcac gtagtgcttc accgatagtt
      781 gagcgatgag cgcgatgagg aacgcaccga acaggacgat gctgctcgcg gcgtagccga
      841 tgttcagcgt catgctcaag aggtccccgg ccgtttcacc caaggtggtg gcgcagatct
      901 tcaaacacca gaacagcagg gtgatctccg gaagcttctt catggtggtg gtgttgccga
      961 gcgaagaggt ggttgaaatt ggcgcgggcc ggaactttcg ccccggcccg ctggaccgat
     1021 cgatcagtcc ttctcgtccg gtccttcgtc cttctcatcc tcttcctcca ccacgctctt
     1081 caacaccttg ccatcggcgg cgaacaccac ttccatgctg tgctcgcctt tctccaggtc
     1141 cacttcgtag gtggtgcctt gcggactttc ggccacctcg gctacttcca ccttgtggtc
     1201 cgcatagctg gtgacgatgg cggtgcgcac ggccggcggc agttcatcca gcttcacggc
     1261 gtgctcggtt tccagccact tggcatcggc agcgtacttc gccgaccatt tcgtgcctcc
     1321 ttgggtgaag tt
//
LOCUS       DAOQOC010001023         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_88119_length_1331_cov_3.032132, whole genome shotgun sequence.
ACCESSION   DAOQOC010001023 DAOQOC010000000
VERSION     DAOQOC010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88119_length_1331_cov_3.032132"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..404)
                     /locus_tag="PL106_14050"
     CDS             complement(204..404)
                     /locus_tag="PL106_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34244.1"
                     /translation="MIHAEGITCVPVWMPVPPGTTTFILVFEPLPDECAVFSLVEEIP
                     EPGGFHVPSIPRNGRDLYDVDV"
     gene            complement(715..>1331)
                     /locus_tag="PL106_14055"
     CDS             complement(715..>1331)
                     /locus_tag="PL106_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34245.1"
                     /translation="KAMFTNTLKTLVSRIGQPLSFLSLKRLLVVSFIALAGTVDIVAQ
                     VHVKGYYRKDGTYVRPHYRSSPDGNPYNNWSYPGNTNPYTGKVATGNPSTYLNNYYHR
                     NGRYYSHRTATLFNTNFRKLERHRSYRITGHSGEQIGMLRYHSGNHFMIVDGSNDCVG
                     WVRMRGPNSYTIVDASGYRVRSKGGGGGIAIGFAALALLVVAIR"
BASE COUNT          293 a          349 c          378 g          311 t
ORIGIN      
        1 tctcctgcga ggagggctta gcctgttgag ccttgaagaa gcggtcaaat atgcccattc
       61 tcgatgtggt caagctgatg tggtcgaggc ttccaaacga tgcgaatgta gtgctatcac
      121 cgtggagggg ctgtcggtct tggaacaggc aaagccgcac atggatttga tcgaatgctc
      181 cttgagatca tatcccaagc gccttataca tccacgtcgt acaagtcacg tccattgcgc
      241 ggaatgcttg gcacatggaa gccgccgggt tcggggatct cttccaccaa gctgaagaca
      301 gcgcattcat caggcaaggg ctcgaagacc agtatgaacg tagtagtacc gggcgggaca
      361 ggcatccata ccggaacaca agtgatgccc tccgcatgta tcatggagct gcgatggcca
      421 gttcgctgac acaccaagta ggtgctgggc catatacgtg cgctggtgcc ccagacggta
      481 cgaatgtggc aacgtatggt gacctgcccg cgctcatcga cctgtgccaa cagttcttca
      541 cggatggcag ggtctaccgg ctccgttttc gtcggaatgg gaaggacttc tgtttcgacg
      601 atcgtgtgag cgtacggggt gatcattggc atgtgtcttc ttgaggttcc gaacaaatgc
      661 ccccgcaggt gacatcatat tggggggcat ctatacgaac caaggagtga gtgtttatcg
      721 aattgcgaca accagcagag ccaaggcagc aaatccgatg gctattccac cgccgccccc
      781 ttttgaacga acacggtagc ctgatgcatc cacgatcgtg tagctattcg ggccgcgcat
      841 cctcacccag ccaacacaat cattactgcc gtccactatc atgaagtggt tcccgctatg
      901 gtatctcaac attccgatct gctcccccga gtgcccggtt atgcgatatg aacggtgcct
      961 ctcaagcttg cggaaattgg tattgaacag ggtcgctgta cggtgagaat agtagcggcc
     1021 atttcgatgg taatagttgt tcaggtatgt gctggggttc ccggttgcca ccttgcctgt
     1081 ataggggttc gtgttccccg gatagctcca gttgttgtag gggttgccat ccggtgacga
     1141 ccggtagtga ggccggacgt aagtgccatc tttccgatag tagcctttga catgtacctg
     1201 agcgacgatg tctaccgtcc ccgccaaggc gatgaaggac accaccagca gccgtttcaa
     1261 gcttagaaag gacaagggct gtccgatccg tgacaccaag gttttgagcg tgttcgtgaa
     1321 catggctttc g
//
LOCUS       DAOQOC010001024         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_88371_length_1329_cov_4.364207, whole genome shotgun sequence.
ACCESSION   DAOQOC010001024 DAOQOC010000000
VERSION     DAOQOC010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88371_length_1329_cov_4.364207"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PL106_14060"
     CDS             <1..656
                     /locus_tag="PL106_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34246.1"
                     /translation="CNYAAVWFRGKGTFTITGIEVDDCPTVFKATQFLDPSDAGHWLL
                     TPGAKLTANGVKVETGVTGSAALRKGTMLFDGIGWCRSTISGVDYASANIYPELLCRI
                     EPFPTGFTSTSSYLKVDGLLIFNFVRTLMHHVQTGNNKKWFVDKGEYTTKFNSSTGGF
                     VYNSANGGTLSVDSGNVVAMKLVPYGNRQKWITLPLGVQQWDDTVNPGTPVYDNPAS"
     gene            656..955
                     /locus_tag="PL106_14065"
     CDS             656..955
                     /locus_tag="PL106_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34247.1"
                     /translation="MSEKTPTGPKGGAAPDMAAEYSIRDEAMRQYYMGVLIDYAAPYD
                     PEYNHDQMRAALITHYQGILDYASEPYDTENDYAAESHNVDYVSYLMRLVNGLGS"
     gene            958..>1329
                     /locus_tag="PL106_14070"
     CDS             958..>1329
                     /locus_tag="PL106_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34248.1"
                     /translation="MAISRNIQEAQAFGYSRCAVASTMCAVEAEIYGDKECGHDCRAM
                     FYWWGVDVLSRTLTEDCDCDDKCVTEAFACTVIRLLNPGCIECGCGSPDEIPTSCDIV
                     ADRSVYQALDASEQNTIPHVAG"
BASE COUNT          278 a          400 c          424 g          227 t
ORIGIN      
        1 actgcaacta cgcggcggta tggttccgtg gcaagggcac gttcaccatc acgggtatcg
       61 aggtggatga ctgccccacg gtgttcaagg caacccagtt cttggacccg tctgatgcgg
      121 ggcactggct gttgacgcct ggcgccaagc tcacggcgaa cggcgttaag gtggaaacgg
      181 gcgttaccgg gagcgctgca ttgcgcaagg gcaccatgct cttcgacggc atcggctggt
      241 gtcgcagcac gatatccggg gtggactacg cctcggccaa catctaccct gagttgctgt
      301 gccggataga gcccttcccc acgggcttca ccagcaccag cagctacctg aaggtggatg
      361 gtctgctcat cttcaacttc gtgcggaccc tcatgcacca cgtgcagact ggcaacaaca
      421 agaagtggtt cgtggacaag ggggaataca ccaccaagtt caactccagc accgggggct
      481 tcgtgtacaa cagcgccaac ggtggcaccc tgagcgtgga cagcggcaac gttgtggcca
      541 tgaagctggt gccctatggc aacaggcaga agtggatcac cctgccgctg ggtgtgcagc
      601 agtgggatga cacggtgaac cccggtactc cggtatacga caaccctgca agctgatgtc
      661 agagaagacc cccacaggcc ccaagggagg tgccgcgccg gacatggcgg cggaatactc
      721 cattcgcgat gaggccatgc gccagtacta catgggtgtg ttgatagact acgcggcgcc
      781 gtacgacccc gagtacaacc atgaccagat gcgcgcggcc ctgatcaccc attaccaggg
      841 catcctggat tacgcatcgg agccctacga cacggagaac gactatgcgg cggagagcca
      901 caacgtggac tacgtgagct acctgatgcg cctggtgaac ggattgggtt cgtgaacatg
      961 gccatatcgc gcaacataca ggaggcgcag gcgttcgggt actcccggtg cgccgtcgcg
     1021 tccaccatgt gcgcggtgga agcggagatc tacggcgaca aggaatgcgg ccacgactgc
     1081 cgcgccatgt tctactggtg gggcgtggac gtactgagcc gcacgctcac cgaggattgc
     1141 gactgcgatg acaagtgcgt gaccgaggcg ttcgcctgca cggtgatccg tctgctgaac
     1201 cccggctgca tcgaatgcgg ctgcggctcg ccggatgaga tacccacatc gtgcgacatc
     1261 gtggcggacc gcagcgtgta ccaggcgctg gacgcctcgg agcagaacac cataccgcac
     1321 gttgccggg
//
LOCUS       DAOQOC010001025         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_88487_length_1329_cov_1.867347, whole genome shotgun sequence.
ACCESSION   DAOQOC010001025 DAOQOC010000000
VERSION     DAOQOC010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88487_length_1329_cov_1.867347"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PL106_14075"
     CDS             complement(<1..1270)
                     /locus_tag="PL106_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA34249.1"
                     /translation="MFLLKNLFRYLGMYFMAPIRTGVVRDLRNELYGKVLVLPLSYFS
                     NERKGDIMARMTNDVQEIEWSIMQSLEVIFREPLTVILFLTTMVLMSPQLSLFVFVLL
                     PIAGILIGQIGKSLRRTSGKSQEQMGNLLSNIEETLGGLRIIRAFTAEEFSFEKFREN
                     NNRFRKLQKRLYRKRDLSSPLSEFLGAVVLVIVMYFGGRLVLSADATLDPSVFIAFIA
                     IFSQLIPPAKAFTEAYSNVQRGLASAERINKILDAEVTIRNRPGAVAIESFKDRIAYR
                     DVSFAYHQGGTGWVLKHISLEVPRGKTIALVGQSGSGKTTLVDLLPRFYELEQGSITI
                     DGQDIRSLTLESLRGLMGIVNQESILFNDTVFNNIAFGMPDATLEDVTRAATIAHAHE
                     FIAHLPEGYQTNIGDRGGKLSGGQRQRIAIA"
BASE COUNT          286 a          379 c          381 g          283 t
ORIGIN      
        1 gggcgatcgc gatacgctgc cgctggccac cgctgagctt tcctccgcga tcaccgatat
       61 tggtctggta accttccggc aaatgcgcta tgaactcgtg ggcatgtgca atggtggcgg
      121 cacgcgtgac gtcttcgagc gtggcatccg gcatgccaaa ggcgatgttg ttgaataccg
      181 tgtcgttgaa caggatgctc tcctggttca caatgcccat cagtccgcgg aggctctcga
      241 gcgtgagtga acggatgtcc tgtccgtcga tggtgatact tccctgctcc aactcataga
      301 agcgcggcag cagatcgacc agcgtggtct tgccggatcc gctttgtccg accagcgcga
      361 tcgttttgcc gcgcggcacc tccaggctga tgtgcttcaa cacccagccg gtaccgccct
      421 ggtgataggc gaaactcaca tcccggtagg cgatgcggtc cttgaaggac tcgatggcaa
      481 cggcacccgg acggttacgg atcgtgactt ccgcgtcgag gatcttgttg atgcgttcgg
      541 ccgaggcgag tcctcgttgc acgttgctat aagcttccgt aaatgccttg gctggcggga
      601 tcaattggga aaagatggcg ataaacgcga tgaacacact cggatcaagt gtggcgtcag
      661 cactcaacac cagtcgaccg ccgaagtaca tgacaatgac caatacgacc gcgccgagga
      721 actccgacaa gggagaactc aggtcgcgtt tccggtagag gcgcttctgt aacttccgga
      781 agcggttgtt gttctcgcgg aacttttcga agctaaactc ctccgcggta aacgcacgga
      841 tgatgcgcaa tccgcccagc gtctcttcga tattgctcag caggttgccc atctgctcct
      901 gcgatttgcc cgatgtccgc cgcaggcttt tcccgatctg accgatgagg atgccggcga
      961 tgggtaataa cacaaaaaca aagagcgata gttgtggact catcagcacc atggtggtga
     1021 ggaagaggat aacggtaagg ggctcgcgga agatcacctc caaggactgc atgatgctcc
     1081 attcgatctc ctgcacgtcg ttggtcatgc gcgccatgat gtcgcctttt cgttcgttgg
     1141 agaaatacga gagcggcagt accaggactt tcccatacag ctcgttgcgc agatcgcgca
     1201 ccacgccggt acggatcggc gccatgaagt acatgccgag gtaacggaac aagttcttca
     1261 acaggaacat caccacgacc agtcccgaga tcacactcag ggcttcgagc ttaccgtgct
     1321 cggccacat
//
LOCUS       DAOQOC010001026         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_88927_length_1325_cov_4.695276, whole genome shotgun sequence.
ACCESSION   DAOQOC010001026 DAOQOC010000000
VERSION     DAOQOC010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88927_length_1325_cov_4.695276"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PL106_14080"
     CDS             <1..600
                     /locus_tag="PL106_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34250.1"
                     /translation="FPSPEIRTRYATDKMGVLMRMDSVFEVWDWQPWPDHAHIFTEAV
                     ISDSLGWTQISGQFNADSAYSFLYLGNFFGDDQTEAILVDPDGNTDISYYYVDDVCLS
                     TDPLDCDIHIGSSEVPILPDIRAWIGQGGVIHLDGLSAQVPIHAMVYDSSGRTVLSWT
                     GGLDNGILSGIGFPSPGTYFLRLIMERSARTFRLIAPNQ"
     gene            711..866
                     /locus_tag="PL106_14085"
     CDS             711..866
                     /locus_tag="PL106_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34251.1"
                     /translation="MKTDASTDGVVIGNLGTPPSPTIGLLVDGNNLFTPTGEVFRTNA
                     PSHTDTY"
     gene            870..>1325
                     /locus_tag="PL106_14090"
     CDS             870..>1325
                     /locus_tag="PL106_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34252.1"
                     /translation="MFHTGTEFGTLYHLDTQGDFNIHATRGYLYLHTTNKLRVRVAPN
                     LAGQTVNNYTGLDLSGFTGIGMFNGPPVHTPFSLLHLDHKGALDGGYRPWMYSGTTCT
                     HFNGWMYTGLKAEDADRVHAVLNWGGNTASVPPVTPQALRFIYTDPSPGT"
BASE COUNT          308 a          390 c          334 g          293 t
ORIGIN      
        1 tttccttcgc cggagatccg taccaggtat gccaccgaca agatgggggt tctaatgcgc
       61 atggactccg tttttgaggt ttgggattgg cagccatggc ccgaccatgc acacatcttc
      121 actgaagctg tcatatcgga ttcactgggc tggacccaga tatcggggca attcaacgcg
      181 gattcagcat attcgttttt gtacctgggt aatttcttcg gtgacgatca gacagaagcg
      241 atccttgtcg atccggacgg gaatacagat atcagctact actacgttga cgatgtatgt
      301 ctgtcgaccg atcccttgga ttgcgacatc catatcggtt ccagtgaagt cccgattctt
      361 ccggacatca gggcatggat aggccaaggt ggagtgatcc acctcgacgg cttaagcgca
      421 caagtcccaa ttcatgcaat ggtctatgat agctcgggac gaacggtttt gtcctggacc
      481 ggggggctcg ataacggcat cttgtcggga ataggatttc catccccagg cacctacttc
      541 ttacgcttaa taatggagcg gtctgcgcga acatttcgtt tgatcgcccc caaccagtaa
      601 caacacctcc acccatgcgg cacacaacaa ggatcctact attactcaca ctcttcacga
      661 tcgtccgcca ggggcattct caagcatgga caacgttggg gttgaactac ttgaagactg
      721 acgccagcac ggacggggtg gtcattggca accttggcac accgccttca cccactattg
      781 gtcttttggt agatggcaac aacctcttca cccccacggg cgaggtattc cgtacgaatg
      841 cacccagcca taccgacacc tactgacgta tgttccacac cgggaccgag ttcggtaccc
      901 tataccactt ggacacgcag ggcgatttca acatccacgc caccagaggc tacctgtacc
      961 tgcacaccac caacaaattg cgcgtgcgcg tggcaccaaa ccttgccggg cagaccgtga
     1021 acaactacac gggtctggac ctgagtggat tcacaggcat cgggatgttc aatggcccac
     1081 ccgttcatac gccgttctcc ctgttgcacc tggaccacaa aggcgccctc gacggtggat
     1141 accggccctg gatgtacagc ggaacaacat gcacccattt caatggatgg atgtacacag
     1201 gactcaaagc tgaagatgcc gaccgcgtcc atgcggtgtt gaactggggt ggcaatacag
     1261 cgagcgtgcc accagtgacg cctcaagcgc tccggttcat ctataccgat ccgtcccctg
     1321 gaacc
//
LOCUS       DAOQOC010001027         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_88944_length_1325_cov_3.570079, whole genome shotgun sequence.
ACCESSION   DAOQOC010001027 DAOQOC010000000
VERSION     DAOQOC010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88944_length_1325_cov_3.570079"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PL106_14095"
     CDS             complement(<1..1007)
                     /locus_tag="PL106_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNA34253.1"
                     /translation="MAKRTRKEDGEPVLLYRTEDGTLRMDVRMDGDTVWLSQEQMAGL
                     FEKDKSTISRHIRNVIAEGELEAVSVIALFATTAADGKTYQVEHYNLDMIISVGYRVN
                     SKRGTQFRIWATRQLREYLIKGFVLDDRRFKEHSALDRYFDELVERIRDIRTSERQFY
                     RKVTDIYATSIDYEPDAEATRKFYATVQNKFHFAITGHTAAELIGGRASSEQPNMGLT
                     NWPGDRILPRDVTIAKNYLNEDELKQLNNIVEQYLAFAESQALRKKPMRMLDWIDKLH
                     GFLTLNEREVLEGLGHLSKDEADDHALREYSKYREANKLGPDHESDFDKAVKRIGKGK
                     P"
     gene            1121..>1325
                     /locus_tag="PL106_14100"
     CDS             1121..>1325
                     /locus_tag="PL106_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34254.1"
                     /translation="MTLPDAGKGAVVEGVEGDCYLGSMAIDIEIDKLTNSIENVRTGE
                     VFDTAVMRITAKDRRLKKMNWRFN"
BASE COUNT          246 a          402 c          357 g          320 t
ORIGIN      
        1 cagggcttgc ctttgccgat gcgcttcacc gccttgtcga aatcgctctc atggtccggt
       61 cccagcttgt ttgcctcacg gtacttgctg tactcgcgca gcgcatggtc atccgcctcg
      121 tccttgctca aatggccaag cccctccagc acttcccgtt cattcagcgt aagaaagcca
      181 tgcagtttat cgatccagtc cagcatgcgc atcggcttct tgcgcaatgc ctgtgattcg
      241 gcaaatgcca ggtactgttc cacgatgttg ttcaactgct tcaattcgtc ctcgttcaag
      301 tagttcttgg cgatcgtcac gtcgcgcgga agaatgcggt cgcccggcca attggtcaag
      361 cccatgttcg gttgttccga gctggccctt ccaccgatca attccgctgc cgtatgtccg
      421 gtgatggcga agtggaactt gttctgcacc gttgcataga acttccgggt agcctccgca
      481 tccggctcat agtcgatgct ggtggcataa atgtccgtca ccttccggta gaactgccgc
      541 tcgctggtgc ggatgtcgcg gatgcgttcg accaattcat cgaagtagcg gtccagtgcg
      601 ctgtgctcct tgaagcggcg gtcgtcgagg acgaagccct tgatcaggta ttcccgtagc
      661 tgccgcgtgg cccagatgcg gaactgggtg ccgcgcttgc tgttcacacg atacccgacc
      721 gagatgatca tgtcgagatt gtaatgttcc acctggtagg ttttaccatc cgcggcagtt
      781 gttgcaaaaa gtgcaataac tgaaacggcc tccaattcac cttcggcgat cacgttcctg
      841 atgtgcctgc tgatagtgga cttgtccttc tcgaatagcc cggccatctg ctcctgtgag
      901 agccaaaccg tatccccgtc catccggaca tccatgcgca acgtgccatc ctccgtacgg
      961 tacagcagca ccggctcccc gtcctccttc cttgttcgtt tggccatgac tttcaggctg
     1021 acgtacttcc cgccaattgc gttcgggcaa gatagcagca gtcgatcacc gcccgaacac
     1081 ctccctccgg tgccgatcca agtactccgg cttcgggccg gtgaccctgc ccgacgctgg
     1141 caagggcgcg gtcgtggagg gcgttgaggg tgattgctat cttggctcaa tggccattga
     1201 catcgagatc gacaagctca cgaactccat cgagaacgtg cgcaccggcg aggtgttcga
     1261 tacggcggtg atgaggatca cagccaagga tcgccgcttg aagaagatga attggcgttt
     1321 caact
//
LOCUS       DAOQOC010001028         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_89378_length_1322_cov_3.004736, whole genome shotgun sequence.
ACCESSION   DAOQOC010001028 DAOQOC010000000
VERSION     DAOQOC010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89378_length_1322_cov_3.004736"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PL106_14105"
     CDS             <1..401
                     /locus_tag="PL106_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNA34255.1"
                     /translation="DPAILGRHPFPGPGLGIRILGEVTAEKVALLQEVDAIWIEGLRE
                     DGLYDQVWQAGAILLPVQSVGVMGDERTYENAVALRAVTSTDGMTADWCDLPHSTLAR
                     ISNNIINRVKGVNRVVYDISSKPPATIEWE"
     gene            398..>1322
                     /locus_tag="PL106_14110"
     CDS             398..>1322
                     /locus_tag="PL106_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA34256.1"
                     /translation="MISRTVLLLAWVLLFASGCALAQDIRTVDGRKHLVHTVMAGQTL
                     YAISRHYAVPVEAITRANPGAAQGLSIGDTLLIPVEAQTRKELKTAPSLAGDELAHTV
                     AKRETLFGIARKYGISEADLLGRNPAARGGLQVGMVLFIPVTKVAGVPPVQTEPAGDH
                     KGTQHLVMPGETLFALAQRYGVPVEAISAANDGLEQGLKAGTYVRIPAKVAEPEPAPV
                     PPAPERPVGGLARVAMLLPFNLAKNDSTVARGNGTKGMYPATDAAVQFYAGALIALDS
                     LKEQGLNVDLSVFEVDDEPEGWRKVQGDPGLQ"
BASE COUNT          248 a          386 c          469 g          219 t
ORIGIN      
        1 tggatcccgc cattctcggg cggcatccct tcccggggcc cggcctgggg atccgtatcc
       61 tgggtgaagt gaccgccgaa aaggtggcct tgctgcaaga ggtggatgcc atctggatcg
      121 agggcttgcg cgaggacggg ctctatgatc aggtgtggca ggccggggcc attctgcttc
      181 cggtccagag cgtgggtgtg atgggggacg agcgcacgta tgagaacgcc gttgccttgc
      241 gggcggtcac cagtaccgac ggtatgaccg ccgactggtg cgacctgccc cacagcaccc
      301 tggcacggat ctcgaacaac atcatcaatc gggtgaaagg cgtgaaccgc gtggtgtacg
      361 acatcagcag caagccgccg gccaccatcg aatgggaatg atcagccgaa cggtcctgct
      421 tctggcttgg gtgctgctct tcgcgagcgg ttgtgcgctg gcacaggaca tccgtacggt
      481 ggatggtcgc aagcacctgg tgcataccgt gatggcgggg cagaccctgt atgcgatcag
      541 caggcactat gccgtaccgg tggaggcgat cacgcgggcg aaccccggtg cagcgcaggg
      601 cctcagcatt ggcgatacgc tgttgatccc cgtggaagcc cagacacgca aggagttgaa
      661 gaccgccccg tccttggctg gcgatgaact ggcccatacc gtggccaaaa gggagacgct
      721 cttcggcatt gcccgcaaat acgggatcag tgaagcggat ctgttggggc gcaacccggc
      781 ggcgcgtggt ggcctgcaag tgggcatggt ccttttcatc ccggtgacca aggtggccgg
      841 ggttcccccg gtgcagaccg aaccggccgg tgaccacaag ggaacccagc acctggtgat
      901 gccgggcgaa acgctgttcg cgctggccca gcgttacggc gtgccggtgg aagcgatctc
      961 cgcggccaac gatggcctgg aacaagggtt gaaggcgggc acgtacgtgc gcatcccggc
     1021 gaaggtggcc gagcccgaac cggcacccgt gccacccgca cccgaacggc cggtgggtgg
     1081 cctggcgcgc gtggccatgt tgctgccgtt caatctggcg aagaacgaca gtacggtagc
     1141 gcgggggaac gggaccaagg gcatgtatcc tgctaccgat gcggccgtac aattctatgc
     1201 cggcgccctg atcgcactgg attcgctgaa ggaacaggga ctgaacgtgg acctcagcgt
     1261 gttcgaggtg gacgatgagc cggaaggatg gaggaaggtg cagggggacc ccgggctcca
     1321 ga
//
LOCUS       DAOQOC010001029         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_89496_length_1321_cov_4.203791, whole genome shotgun sequence.
ACCESSION   DAOQOC010001029 DAOQOC010000000
VERSION     DAOQOC010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89496_length_1321_cov_4.203791"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PL106_14115"
     CDS             <1..1109
                     /locus_tag="PL106_14115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA34257.1"
                     /translation="DYTDREVFARLTVIETDGPTFSQSLDPQSIPDAIAYVPVSNTSW
                     SLINNECLTTTGFLGNDTTACSAIMLDASPSEATAYQWQDGSTNATFTATGPGTYWVI
                     ASSDCCISSDTVVVTGGAPPTALFEVQIDPCLFDATFNNMSIHADQFEWQFGDGTTSS
                     EVSPVNTYDSTGSYTVSLIASNACGNDTSSLTIGIGALGMIALQGPALICDGEQASYS
                     VTVHGVGMDDVTWSTGDNTSSILLSLPVDQLLSVAVTGSDGCLYAAALNVQVIGLAGE
                     AAAYVPNVFTPNGDGLNETLAPVLSDPETLIELLIFNRWGQEIFTTTSVQQPWNGKVE
                     GGPVPDGTYIYIVRWKDRCTGTRKEKYGHVTLLR"
     gene            complement(1116..>1321)
                     /locus_tag="PL106_14120"
     CDS             complement(1116..>1321)
                     /locus_tag="PL106_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34258.1"
                     /translation="RPILAFGPQDGDVARVLEGHVLVDRREVHPDPLMIAKLFERTGG
                     GGVNADFSRSAGAGRMATILAGA"
BASE COUNT          266 a          429 c          373 g          253 t
ORIGIN      
        1 gcgactacac cgaccgcgaa gttttcgccc gtctcaccgt catcgagacc gatggcccca
       61 ccttcagcca gtcgttggat ccgcagtcca tacccgatgc catcgcgtat gtgccagtga
      121 gcaacacgtc atggagcctt atcaacaacg agtgccttac cacaaccggg ttcctgggca
      181 acgacaccac agcctgttcc gccatcatgc tcgacgcctc gccctccgag gccaccgcgt
      241 accagtggca ggacggatca acgaacgcga cctttaccgc gacaggcccc ggtacgtact
      301 gggtgatcgc ctcgagcgac tgctgcatct ccagcgatac cgtggtcgtg accggtgggg
      361 cgccaccaac ggccttgttc gaggtgcaga tagacccttg cctgttcgat gcgaccttca
      421 acaacatgtc catccatgcg gaccagttcg agtggcaatt cggagatggc acgaccagtt
      481 ctgaagtgtc tcccgtgaac acctacgact ccacgggcag ctacaccgtg tccctcatcg
      541 cctccaatgc gtgcggcaat gatacctctt ccctcaccat cgggatcggt gccttgggca
      601 tgatcgccct gcaaggtcct gcattgatct gcgatggcga gcaggcgagt tattcggtga
      661 ctgttcacgg tgttggcatg gatgatgtga catggtcgac gggagataac acctcgtcga
      721 tcctgctgtc cctcccggtc gatcaactcc tgagtgtggc cgtgacaggt agcgatggct
      781 gcctgtacgc ggccgcgttg aacgtgcagg tgatcggtct ggccggtgaa gcggcggcct
      841 acgtcccgaa cgttttcacg ccgaatgggg atgggttgaa cgagactctc gcaccggtat
      901 tgagcgaccc ggagacactg atcgaactgc tcattttcaa tcgctggggg caggagatct
      961 tcacaaccac atccgtacag caaccgtgga acgggaaagt ggaaggcggg cccgtaccgg
     1021 acggcaccta catctacatc gtgcgttgga aagaccggtg taccggcacg cggaaagaga
     1081 aatacggtca cgtgacgctg ctccgttgag ccggttcacg caccggccag gatcgtggcc
     1141 atccttccgg ctcccgccga tcgcgaaaaa tcggcattca cccctccccc gccggtccgt
     1201 tcgaagagct tcgcgatcat caaagggtcc ggatgcacct cccggcgatc gaccaacacg
     1261 tgtccttcca gcacccgcgc cacatcacca tcctgcggcc cgaaagccag gatgggcctg
     1321 c
//
LOCUS       DAOQOC010001030         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_89951_length_1318_cov_4.053048, whole genome shotgun sequence.
ACCESSION   DAOQOC010001030 DAOQOC010000000
VERSION     DAOQOC010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89951_length_1318_cov_4.053048"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>1318)
                     /locus_tag="PL106_14125"
     CDS             complement(131..>1318)
                     /locus_tag="PL106_14125"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNA34259.1"
                     /translation="RIIYPANDPGMLRPALAALNAAATSPHAVHVLHYGDSQLEGDRI
                     TSYLRNKFQLRWGGGGMGLLPIIDVGPSFSVDREPTENWERFSVMEHHDPPLGHQRYG
                     ALWSFCRFTPVVADSLINDSVLQEGRIVLRKVGRAYGRSQRYTRCRVFFAHNHRPVTL
                     SVGADDAVPENREFSAGDMLRIAEFNVNAHASLDVTFTGSDSPEVYGFSLESTSGVVV
                     DNLAARGAGGTELRNVEKTLLKAMYTALDVELLILQFGGNAVPHIESAQDAEEYGAWL
                     GSQIVTWRTLCGGVPVILIGPSDMSIKDGTGWTTRPWLEEVNAALKKHALANGAAYFD
                     LFAAMGGRNSMVSWVEADPPLAAPDYTHFSPQGASRMAELFCEALFNDLDGLNEHVAS
                     HAD"
BASE COUNT          235 a          428 c          411 g          244 t
ORIGIN      
        1 ccgcgacagg tcgggcaagg cgttgtccgg cacgatcagc gggtcgctgg atgattgggc
       61 caccatgatc ccagtgcaca acagggtgat gcacgacacg atgagccttg gtaccggcgt
      121 cgcggaaggt tcagtccgca tgggatgcaa catgttcgtt caatccgtcc agatcgttga
      181 agagcgcttc gcagaacagc tccgccatcc ggctggcgcc ctgcgggctg aagtgcgtgt
      241 agtcgggtgc ggccaacggc ggatcggcct ccacccagct caccatggag tttcttccgc
      301 ccatggccgc gaacagatcg aagtacgctg cgccgttggc cagagcgtgt ttcttcagcg
      361 cggcgttcac ttcttccagc catgggcggg tggtccatcc cgtaccgtcc ttgatgctca
      421 tgtcgctggg accgatcagg atcacgggaa caccgccgca gagcgttcgc caggtgacga
      481 tctgcgaacc cagccaggcc ccgtactcct cggcgtcctg cgcgctttcg atatgcggca
      541 cggcattccc gccgaattgc aggatgagca gttccacgtc caaggcggtg tacatggcct
      601 tcaacagcgt cttttccacg ttgcgcagct cggtgcctcc ggccccacgg gccgcgaggt
      661 tgtccaccac cacgccgctc gtgctctcaa ggctgaagcc gtacacttcc ggactgtcgc
      721 ttccggtgaa ggtcacatcc aaggacgcgt gtgcgttgac gttgaactcg gcgatacgca
      781 gcatatcgcc cgcgctgaac tccctgtttt ccggtacagc atcatcggcc ccgacgctca
      841 gcgtcaccgg ccggtggttg tgcgcgaaga agacacggca tcgcgtgtac cgctgggagc
      901 gcccgtaggc acgccccacc ttgcgaagca cgatgcgccc ctcctgcaac acgctgtcgt
      961 tgatcaggct atcggccact accggtgtga agcggcagaa cgaccagagc gccccgtacc
     1021 gctgatgacc gagcgggggg tcatgatgtt ccatcacact gaaccgctcc cagttctccg
     1081 tcggttcgcg gtccacgctg aaggaggggc ccacgtcaat gatggggagc aggcccatgc
     1141 caccaccgcc ccaacgcaac tggaacttgt tgcgcaggta gctggtgatg cggtcgccct
     1201 cgagctggga gtcgccgtaa tgcagcacat gcacggcatg cggcgatgtg gcggcggcat
     1261 tgagcgcggc cagtgcgggc cgcaacatgc ccgggtcatt ggcgggataa atgatcct
//
LOCUS       DAOQOC010001031         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_90113_length_1317_cov_3.353407, whole genome shotgun sequence.
ACCESSION   DAOQOC010001031 DAOQOC010000000
VERSION     DAOQOC010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90113_length_1317_cov_3.353407"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PL106_14130"
     CDS             complement(<1..268)
                     /locus_tag="PL106_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HNA34260.1"
                     /translation="MRRCLQLARNGTGAVAPNPLVGAVLTRGERILAEGWHRACGGPH
                     AEVECLRAFGDGPVPADAVMYVNLEPCAHHGRTPPCIDLLIARGV"
     gene            329..1192
                     /gene="prmC"
                     /locus_tag="PL106_14135"
     CDS             329..1192
                     /gene="prmC"
                     /locus_tag="PL106_14135"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNA34261.1"
                     /translation="MDVRVATNTVRAVVDLYRRDLAEMYPEGEVRAMVRAVFQDRLGW
                     GPVELMSRHDSALSESEILRVHLPLDRLRRGEPLQYVLGHTLFHGLHLEVGPSVLIPR
                     PETEELVDLIIRSCRVPPRCIIDIGTGSGCIALALKQRFPTALVIGIDVSDGALAVAS
                     ANAARNGLDVEWEQLDVLQEGIRLPAADLIVSNPPYVPLTELDTLAPNVRDHEPHLAL
                     FVDDADPLRFHRAIARHALNALDPDGALWFEGHHLNVLAGAEMLRELGFPRTTTAMDL
                     GGLPRFIHARR"
BASE COUNT          233 a          415 c          428 g          241 t
ORIGIN      
        1 ccacaccacg agcgatgagc agatcgatgc acggcggtgt tcgaccatga tgtgcgcagg
       61 gttcgaggtt cacgtacatg acggcatcgg ccgggacggg cccatcgccg aatgcacgca
      121 ggcattcgac ctcggcatgc ggaccaccgc acgcgcggtg ccagccctcg gccaggatcc
      181 gctcgccccg cgtgagtacc gcgcccacca acggattcgg agccaccgca ccggtgccgt
      241 tccgcgccag ttgaaggcag cgccgcatga tctcttcctg gaacacggcc gcgaaggaaa
      301 ggcatccgct cctcgttact ttgcggccat ggacgtgcgg gtcgcaacga acacggtgcg
      361 cgcggtggtc gatctgtacc gacgcgacct ggcggagatg tacccggaag gggaggtgcg
      421 cgccatggtc cgtgccgtat tccaggaccg gttgggatgg gggccggtgg aactgatgag
      481 ccgccacgac agtgccctca gcgagagtga gatcctgcgt gtgcaccttc cgctggaccg
      541 ccttcgtcgg ggcgaaccgc ttcaatacgt actcggccat acattatttc atgggctcca
      601 cctggaagtc gggccttcgg tcctcattcc acgaccggag acggaagaac tggtggacct
      661 catcatccgc tcgtgccggg tgccaccgcg atgcatcatc gacatcggta cgggcagtgg
      721 gtgcatcgca ctggcactga agcaacgctt cccgacggcg ctggtcatcg gtatcgatgt
      781 gagcgacggt gccctggcgg tggccagtgc caatgcggcg cgcaacggac tggatgttga
      841 atgggaacaa cttgatgtgc tgcaagaggg gatcaggctc cccgcggccg atctgatcgt
      901 gagcaacccg ccgtacgttc cgctcaccga actggacacc ctcgccccga atgtgcgcga
      961 ccatgaaccg cacctcgcgc ttttcgtgga cgatgcggat ccactgcgct tccaccgggc
     1021 gatcgcgcgt catgcgctca atgccctgga cccggacggt gcgctctggt tcgaggggca
     1081 tcacctgaat gtgctggcag gtgcggagat gctgcgcgaa ctcggcttcc caaggacgac
     1141 cacggccatg gatctgggcg gtctgccgcg tttcatccac gcgcgccgat gagggtgttg
     1201 ctggtcgtat gcctctgttc tggtatcata catggactgt gggcccaggt gggcgatacc
     1261 acgcgtgcgc tcgatgtgcg ggacaagccc aaattcatcc tcaattttga tgcgcgg
//
LOCUS       DAOQOC010001032         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_90136_length_1317_cov_2.908875, whole genome shotgun sequence.
ACCESSION   DAOQOC010001032 DAOQOC010000000
VERSION     DAOQOC010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90136_length_1317_cov_2.908875"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..414)
                     /locus_tag="PL106_14140"
     CDS             complement(82..414)
                     /locus_tag="PL106_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNA34262.1"
                     /translation="MNVENTKAQMRKGVLEYCILSLLSGEDLYPGDIITRLKEAKMIV
                     VEGTLYPLLTRLKDAGFLTYRWEESRSGPPRKYYQLTPVGRKFLKELDDTWDELAAAV
                     KKTTRNNP"
     gene            complement(792..>1317)
                     /locus_tag="PL106_14145"
     CDS             complement(792..>1317)
                     /locus_tag="PL106_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34263.1"
                     /translation="GVTAHTASMASNPNMSPEKARAYIDTVMGYMNPRIMAALQLDQP
                     CDLMIPDCLGVPGGTALPGTPCDDSDPNTLDDTWSEGCDCVGIPDAIAENQGLSASVT
                     LMPNPVHDQVTITSSKARILGYELYDATGRRVRTANVNADRFTLPRTGLQAGAYFIIL
                     RFEQGNVTRKLALD"
BASE COUNT          212 a          376 c          446 g          283 t
ORIGIN      
        1 agaccgtgcc ggcgatgttg gcggtgactg tctttttcat ggttttccgg ggttggtgcg
       61 tggtcgatca cgcccttgcg atcagggatt gttgcgggtg gtttttttca cggcggcggc
      121 cagttcgtcc caggtgtcgt ccagttcctt caggaatttc cgaccaacag gggtgagctg
      181 gtagtatttg cgcggtgggc cgctgcggct ttcctcccag cggtacgtga ggaaacccgc
      241 atccttcagc cgggtgagca gcgggtaaag ggtgccttcc accacgatca tcttcgcctc
      301 tttcaacctg gtgatgatgt cgcccgggta cagatcctcg ccgctcagca gcgagaggat
      361 acagtattcg agcacgccct tgcgcatctg cgctttggtg ttctccacgt tcaggttcat
      421 gcttctggcc ggttatgggt ctcccgtggt actatgacga cgcaaagtaa atgcttttgc
      481 ttggtactat gcaagacaaa gtaccttcca ttggatgaac ggacgcggga cagggatgag
      541 cggcaggggt gcccgtcgta cacccatcat ggaagagaac ggaccttgtt ccgatgtggg
      601 cttcgcgcag gcgccgtgga tacggacgga cctttgttga ggagccgctg gacgtgccgg
      661 gcctgtgcat gggtcggcat ggtgcgccat gagcaccgat cgcgcatgga cgtgggcgcc
      721 cgaacggacc gggaccaaag acagagcccc caccgccggg cggtgagggc tccagtgtga
      781 agctgttgcg cttaatccag tgccaacttg cgcgtcacgt tcccctgctc gaaccgcagg
      841 atgatgaagt aggcgccggc ttgcaggccg gtgcgaggca gggtgaagcg gtccgcgttc
      901 acgttggccg tgcgcacgcg gcggccggtg gcgtcgtaca actcatagcc caggatacgg
      961 gccttgctgc tggtgatggt cacctggtcg tgtaccgggt tgggcatcag ggtcacgctg
     1021 gccgaaaggc cctggttctc cgcgatcgca tcggggatgc ccacgcagtc gcatccctcg
     1081 ctccaagtgt cgtccagcgt gttcgggtcg ctgtcgtcgc aaggggtgcc gggaagcgcg
     1141 gtgccgccgg gcacacccaa gcagtccggt atcatcaggt cgcagggctg atccagttgc
     1201 agagcggcca tgatccgcgg attcatgtag cccatgaccg tgtcgatgta cgcgcgggcc
     1261 ttctcggggc tcatgttcgg gttgctcgcc atggaggccg tgtgcgccgt aacgccg
//
LOCUS       DAOQOC010001033         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_90158_length_1317_cov_2.624406, whole genome shotgun sequence.
ACCESSION   DAOQOC010001033 DAOQOC010000000
VERSION     DAOQOC010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90158_length_1317_cov_2.624406"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PL106_14150"
     CDS             <1..792
                     /locus_tag="PL106_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34264.1"
                     /translation="ALTADRLSMQHARWTWAATLLLILPARTLWTANLDHTLAWPEQA
                     VPERFMRQTLGSGDAPGHARLVGGQHQLALVWPMNAYWQGLPVPPMQVVGFPKGPHDL
                     RIVDERYLREALPGYHAIDSAKGPGLWLLERDTPLSMTLAITLVSPARSTRDEFDELA
                     HIPDTLLHAHAVQLVVDVPLSLIPSAPDLRLVLEVNDREGNKLFYDAMGPLALRPNWR
                     GEPLHWTWRLPALPGASRAVLYFHNPGKVMLARGIAKVAVSTIRE"
     gene            831..>1317
                     /locus_tag="PL106_14155"
     CDS             831..>1317
                     /locus_tag="PL106_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNA34265.1"
                     /translation="MALDKNQMAKRIARELKDGWYVNLGIGIPTLVANHIPQGINVTL
                     QSENGILGMGPFPWEGEEDADLINAGKQTVTLLPGSSIFDSATSFAMIRAQKVQLTVL
                     GAMEVADNGDIANWKIPGKMVKGMGGAMDLVASAENIIVCMLHTNKAGETSVSNASEV
                     IL"
BASE COUNT          247 a          429 c          412 g          229 t
ORIGIN      
        1 gcactgaccg cggatcgcct atccatgcaa catgcgcgat ggacctgggc cgccacgctg
       61 ctattgatcc tgcccgcccg taccctgtgg acggcgaacc tggaccacac cctggcctgg
      121 ccggagcagg ccgtgcccga acgcttcatg cggcagacgt tggggagtgg cgatgccccc
      181 ggacacgccc gacttgtcgg cggacagcac caactggccc tggtatggcc gatgaacgct
      241 tactggcagg ggcttccggt acctcccatg caggtcgtcg gttttcccaa aggtccgcac
      301 gatctgcgga tcgtggatga gcgctacctg cgcgaagccc tgccaggtta ccacgccatc
      361 gacagcgcca agggtccggg gctctggttg ttggaacgcg acaccccgct ctccatgacc
      421 ctggccatca cgctggtttc gcccgcgcgg agcacgcgcg acgaattcga cgaactggcg
      481 cacatacccg acacgctgct ccacgcccat gccgtccagt tggtagtgga tgttccgttg
      541 agcttgatcc catcggcacc cgatctgcga ctggtgctgg aggtgaacga ccgtgagggc
      601 aacaaactct tctacgatgc catgggtccg ctggccctgc gccccaactg gcgcggcgag
      661 ccgcttcatt ggacctggcg cctgcccgca cttcccggtg ccagccgggc ggtgctctac
      721 ttccacaacc ccgggaaggt catgctcgca cgcggcatcg ccaaggtggc cgtgagcact
      781 atccgggaat agatgcgcat cttcgctcgc gatatccatt ccccttttcc atggcattgg
      841 acaagaacca gatggcgaag cgcatcgcgc gcgaattgaa ggacggctgg tacgtgaatc
      901 tcggtatcgg catccccacc ttggtggcca accacatccc gcagggcatc aacgtgacgc
      961 tgcagagcga gaacgggatc ctcggcatgg gcccgttccc gtgggagggc gaggaggatg
     1021 cggacctgat caatgccggc aagcagaccg tgacgctgct gcccggcagc agcatcttcg
     1081 acagtgccac cagctttgcc atgatccggg cccagaaggt gcagctcacc gtgctggggg
     1141 ccatggaggt ggcggacaat ggcgacatcg ccaactggaa gatccccggg aagatggtga
     1201 agggcatggg cggggccatg gacctggtgg cgagcgcgga gaacatcatc gtgtgcatgc
     1261 tgcacaccaa caaggccggc gagacatccg tttccaacgc ttcagaagtt attctcg
//
LOCUS       DAOQOC010001034         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_90267_length_1316_cov_4.088025, whole genome shotgun sequence.
ACCESSION   DAOQOC010001034 DAOQOC010000000
VERSION     DAOQOC010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90267_length_1316_cov_4.088025"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PL106_14160"
     CDS             <1..535
                     /locus_tag="PL106_14160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNA34266.1"
                     /translation="QADIAALTTVDPDPINANISGKVLPNVGFGIMLHSPRYYVGLSA
                     PKLLENTIGTDGGVVTNREFRHFFLQAGYVLDLSRDLKFKPSIMARAVQGAPISMDLN
                     ANFLLRDKVWFGAMYRLGNGAGVMAQYNFNEQLRAGYAFDLTTTRIGAYNAGTHEIML
                     SYDFRFDRGRTISPRYF"
     gene            535..>1316
                     /locus_tag="PL106_14165"
     CDS             535..>1316
                     /locus_tag="PL106_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA34267.1"
                     /translation="MGRLLRHILLLLLALSPVVPVHAQEGGSPEAHTRIADRHYQRMA
                     YALAAAEYRTAADLGATNEHVAKRLADCYMKLGDTERAETWYAQVVRYLNVQPIDYYN
                     YAQALKGNGKYAEAETWMDKYLDTARPEGVAPHSNISDFARKFTRGMDRFTVRSVSIN
                     TAYCDMATAWDGSDAVIFSSSRNESTVIKRQAAWNGQPFLDLFTAQRQPSGDLVDAQP
                     LRGGLNGPLHEGPVALDASGGTLWYTRNSAVRGRNGTVRLSI"
BASE COUNT          255 a          417 c          413 g          231 t
ORIGIN      
        1 ccaggccgac atcgcggcgc tcaccacggt ggaccccgac cccatcaacg ccaacatcag
       61 cgggaaggtg ttgcccaacg tgggcttcgg tatcatgttg cactccccgc ggtactacgt
      121 gggcctcagc gcacccaaac tgctggagaa cacgatcggc acggacggtg gcgtggtaac
      181 gaaccgtgaa ttccgccatt tcttccttca agccggctac gtcctcgacc tgagccgcga
      241 tctgaagttc aagccgtcca tcatggcacg agcggttcag ggggccccga tctccatgga
      301 cctgaacgcg aatttcctgc tgcgcgacaa ggtctggttc ggggccatgt accgcttggg
      361 caatggagcc ggggtgatgg cgcagtacaa tttcaacgag cagttgcgcg ccggatacgc
      421 cttcgacctc accactacgc gcatcggcgc gtacaacgcg ggcacgcacg agatcatgct
      481 ctcctacgac ttccgcttcg accggggccg caccatttcg ccgcgctatt tctgatgggc
      541 aggcttctcc ggcatatcct cctcctgctc ctggcgctgt cgcccgttgt ccccgtccat
      601 gcgcaggagg gtggaagccc cgaagcgcat acgcgcatcg cggatcgcca ctaccagagg
      661 atggcgtacg ccctggccgc ggccgagtac agaacagcgg cggacctggg agcgaccaat
      721 gagcatgtgg cgaagcgact ggcggattgc tacatgaaat tgggggacac ggagcgggcc
      781 gagacctggt acgcgcaggt ggtgcgctac ctgaacgtgc agccgatcga ttactacaat
      841 tatgcgcagg cattgaaagg gaacggcaaa tacgccgagg cggagacctg gatggacaag
      901 tacctggaca ccgcgcggcc cgaaggtgtc gcgccccaca gcaacatcag cgatttcgca
      961 cggaagttca cgcgtggcat ggaccgtttc acggtgcgtt ccgtgtccat caacacggcg
     1021 tactgcgaca tggccacggc gtgggatgga tcggacgccg tgatcttcag cagcagccgg
     1081 aacgagtcca ccgtgatcaa gcgccaggcg gcatggaacg gtcagccttt tctggatctt
     1141 ttcacggcgc agcggcagcc ttccggcgac ctggtggatg cgcaaccgct gcgcggcgga
     1201 ttgaacggcc cgctgcacga gggcccggta gctctcgacg cgtccggtgg cacattgtgg
     1261 tacacgcgca acagtgctgt ccgcgggcgc aatggaacgg tccgtctcag catctt
//
LOCUS       DAOQOC010001035         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_90813_length_1313_cov_1.856121, whole genome shotgun sequence.
ACCESSION   DAOQOC010001035 DAOQOC010000000
VERSION     DAOQOC010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90813_length_1313_cov_1.856121"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PL106_14170"
     CDS             <1..421
                     /locus_tag="PL106_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34268.1"
                     /translation="LERVFLAVPWSRRFERQVRVLGAFGGQRLTVVATLSGLRYAVFT
                     LQFAWLLHTVGAVPWSTACARVPVIFLVTTLIPTTALTELGVRGSVTDTLVSGGDPVG
                     VVVSSILIWAVNIALPALVGSLILLVARIRTESPDAG"
     gene            418..>1313
                     /locus_tag="PL106_14175"
     CDS             418..>1313
                     /locus_tag="PL106_14175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA34269.1"
                     /translation="MMTTALVILAFGLAVLFATMVRGWCEDVARALMDAPDAATGTSA
                     TVAHIVPARDAEDTIVPLLQDLYEQGRPGHPPSVIVVDDGSSDGTADKVRAMIPRWSG
                     LRLLPCDGQGKKAAITTGVNATDAGWIVLTDADARCGPDRTRLLLACAERTRADMVLL
                     PVATDGQGVLGAIQSNEQAALMMCAMATTLQGRPLLANGANMAFRREAFLELGGYNGD
                     RFASGDDVFLVQRMMRAGKRIAALPHPDAVVRTQAEPTFSGAVQQRLRWAGKMRGVSW
                     SAKLLPAFFLLYPWGLLALTVG"
BASE COUNT          185 a          427 c          449 g          252 t
ORIGIN      
        1 cttggagcgg gtcttcctgg ctgttccctg gtcccgccgg ttcgaacggc aggtccgcgt
       61 gctcggtgcg ttcggtggcc agcgactcac ggtggtggcc acgctgagcg ggctgcgcta
      121 tgccgtcttc accctccagt tcgcctggtt gctgcacacg gtgggcgccg tgccctggtc
      181 cacggcatgc gcccgggtac ccgtgatctt cctggtcacc acgctcatcc ccaccaccgc
      241 cctcaccgaa ctgggcgtgc gcggttcggt tacggacacg ctcgtcagcg gcggcgatcc
      301 cgtgggtgtg gtcgtgtcct cgatcctgat ctgggccgtg aacatcgcgc tgcccgccct
      361 tgtcggcagc ctcatcctgc tcgtggcccg gatccgcacc gaaagcccgg acgccggatg
      421 atgaccacgg ccctggtgat cctggcattc ggcctggccg tgctcttcgc caccatggtc
      481 cgtggctggt gcgaggatgt tgcacgcgcc ttgatggatg cgccggatgc cgcgaccggc
      541 acttcggcca ccgtggccca cattgtgccc gcacgcgatg cggaggatac cattgtaccg
      601 ttgttgcagg atctgtacga acagggacgt cccggacatc cgccgagcgt gatcgtggtg
      661 gatgatggca gcagcgatgg cacggcggac aaggtgcgcg ccatgatccc gcgctggtcg
      721 gggttgaggc tcctgccttg cgatggccaa gggaagaagg cggcgatcac caccggcgtg
      781 aacgcgaccg atgccggttg gatcgtgctc accgacgccg atgcgcgctg cggtcccgat
      841 cggacgcgcc tgctgctcgc ctgtgcggag cgtacccggg ccgacatggt cctgctgccc
      901 gtggccacgg atggacaggg agtgcttggc gccattcagt cgaatgaaca ggccgccctg
      961 atgatgtgcg cgatggccac caccctgcaa gggcgtcccc tgctcgccaa tggcgcgaac
     1021 atggcctttc ggcgcgaggc gttccttgaa ctgggcggat acaacggtga tcgttttgcc
     1081 agcggcgacg atgtgttcct ggtgcaacgg atgatgcggg cgggcaaacg catcgcggca
     1141 ttgccgcatc cggatgcggt ggtgcgtacg caggccgagc caacgttcag cggggctgtt
     1201 caacaacgtc tgcgctgggc gggcaagatg cgcggcgtgt cctggagcgc caagctgctg
     1261 cccgctttct tcctgcttta cccgtgggga ctgctcgcac taacggtcgg tat
//
LOCUS       DAOQOC010001036         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_91002_length_1311_cov_3.796178, whole genome shotgun sequence.
ACCESSION   DAOQOC010001036 DAOQOC010000000
VERSION     DAOQOC010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91002_length_1311_cov_3.796178"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PL106_14180"
     CDS             <1..961
                     /locus_tag="PL106_14180"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNA34270.1"
                     /translation="PQLDIFSAGNGENLLAEWTGTVHPERKVIICGHYDSMPAGPVAS
                     PAADDDGSGTVAVLEAMRVLTQHSFENSIVFAFWDMEEQGKLGSINYANGIDMDETPV
                     VGVVNLDAIAYDGDGDGLMRVHTKNIANSIALKDTAVLVNQTYTGLQLPMGINLPGAT
                     YSDHASFWSRGIGAILIIEDFDDDANPHYHTTTDLPEFIDEPYFHGLARLGIGTTAVL
                     ARPVPEDPMEVTATSGKDPGLSVYPNPMEGTVTVRIPPGPGLTGLDLYDAMGRHVLDL
                     TPGAVSGNSRLYQVNASALAPGTYVLRSVRDGRVTSLPVVRTP"
     gene            complement(1016..>1311)
                     /locus_tag="PL106_14185"
     CDS             complement(1016..>1311)
                     /locus_tag="PL106_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34271.1"
                     /translation="HGMIIVQEGTGVHEAVADPGAITLFPVPSSGRLNVSLEGKNYRM
                     AQIIGADGRTIDERSIQPGVLNGIDISKLPNGRYMLRLMGDNGNIILRPFVKE"
BASE COUNT          257 a          395 c          421 g          238 t
ORIGIN      
        1 gccccagctc gacatcttca gtgccggcaa tggcgagaac ctgttggccg aatggaccgg
       61 gaccgtacac cctgaacgga aggtgatcat ttgcggccac tacgattcga tgcctgccgg
      121 accggtagcc tccccggccg ccgacgatga cggtagcggc acggtcgctg tgctcgaggc
      181 catgcgggta ctgacgcagc acagctttga gaactccatc gtattcgcct tctgggacat
      241 ggaggaacag ggtaagctgg gcagtatcaa ctacgcgaac ggtatcgaca tggacgagac
      301 acccgtggtg ggggtggtga acctggacgc catcgcctat gacggggatg gggatggcct
      361 gatgcgcgtg catacgaaga acatcgccaa cagcatcgcg ctgaaggaca ccgccgtgct
      421 ggtgaaccag acctacaccg gcctccagtt gcccatgggg atcaacctgc ccggggcaac
      481 gtacagcgat catgcctcct tctggagccg aggcatcggc gcgatcctga tcatcgagga
      541 tttcgatgat gatgcgaacc cgcattacca caccaccacg gacctgccgg aattcatcga
      601 tgaaccgtat ttccacgggc ttgcgcggct cggcatagga accacggcgg tattggcccg
      661 accggtcccc gaggatccca tggaggtgac agcaaccagt ggaaaagatc ccggactgtc
      721 cgtgtatccg aacccgatgg agggtactgt cacggtgcgc ataccaccgg ggcccggtct
      781 gacgggcttg gacctgtatg acgcgatggg tcggcatgtg ctggacctca caccaggggc
      841 ggtgagcggg aacagtcgac tgtaccaggt caatgcgagc gcgcttgctc cgggaacgta
      901 tgtgcttcgg tccgtccgcg atggccgggt aacgagcctg ccggtggtgc gtaccccgtg
      961 aaacggaaca gcccccgccc ggggcggagg ccgttgccgg gcggagccgt caatgctatt
     1021 ccttcacgaa tggacgcagg atgatgttcc cgttgtcgcc catgagccgg agcatgtacc
     1081 gtccgttcgg gagttttgaa atatcgatcc cgttcaacac gccgggctgg atgctgcgct
     1141 cgtcaatggt ccggccatcg gcaccgatga tctgtgccat ccggtaattt tttccctcga
     1201 ggctgacgtt cagacggccc gaagagggga ccgggaagag ggtgatggca cccgggtcgg
     1261 ccacggcctc atgcaccccg gtgccctcct gcacgatgat catgccgtgc t
//
LOCUS       DAOQOC010001037         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_91173_length_1310_cov_3.065339, whole genome shotgun sequence.
ACCESSION   DAOQOC010001037 DAOQOC010000000
VERSION     DAOQOC010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91173_length_1310_cov_3.065339"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PL106_14190"
     CDS             <1..882
                     /locus_tag="PL106_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34272.1"
                     /translation="LLPGLLQWSVPSIAAYSNFVVEAVFLVPTTTQIGATVPAIASIT
                     LLDPDAQPDNDQYSAVRNVTSSFDPNDKLCVTSSRLSATSYYLDADSYVDYTIRFQNT
                     GTGPAYNVHLLDTITPLLDLTSFTILAASHDYTASLGDARDLRFDFPNIMLPDSGSNM
                     AGSQGFIAFRLKPVESIELGDELVNAADIFFDFNEPVRTNDAVLVVDHFSGVEEPDAA
                     ALAVYPNPVTDLLHINLTGDVVRMDVISVDGRMVLSQRAISGANQFDTRALTPGAYSL
                     RVLSASGTVRHARFVKR"
     gene            927..>1310
                     /locus_tag="PL106_14195"
     CDS             927..>1310
                     /locus_tag="PL106_14195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNA34273.1"
                     /translation="MPKRFLVIQTAFLGDAVLATALLEKLHAFFPDAAVDLVVRKGND
                     GLFDGHPFLRKVFVWDKRTNKTRNLFKLIGALREERYDHVINCQRFFSTGLMTVLARA
                     DEKIGYDKNPLSFLVSRKLKHVIGDG"
BASE COUNT          257 a          429 c          342 g          282 t
ORIGIN      
        1 ctgcttcccg gacttttgca atggtccgta ccttccatcg ccgcatactc caactttgtc
       61 gtagaggccg tgtttctggt accgaccacc acccagatcg gagccactgt tcctgctatc
      121 gcctccatca ccctgctcga tccggatgcg caacccgaca atgatcagta ctccgcggtg
      181 cggaatgtca cctcctcttt tgaccccaat gacaaactct gtgtgaccag ttcccgcctc
      241 agcgccacca gctactacct cgatgcggac tcatacgtgg actacaccat ccgcttccag
      301 aacaccggta ccggacctgc atacaacgta cacttgctgg atacgatcac cccgctgctg
      361 gacctcactt cgttcaccat tctggccgca tcccacgact acaccgcatc gctcggcgat
      421 gcacgtgatc tgcggttcga cttcccgaac atcatgttgc ccgatagcgg tagcaacatg
      481 gccggcagcc aaggtttcat cgccttccgc ctgaaaccgg tggagtccat cgagttgggc
      541 gatgaactgg tgaacgccgc ggacatcttc ttcgacttca atgagccggt gcgcaccaac
      601 gatgccgtgc ttgtggtcga ccatttctcc ggcgtcgagg agcctgacgc agccgcgctc
      661 gccgtatacc cgaaccccgt gacggacctg ctgcacatca acctgacagg cgacgtggtc
      721 cgcatggacg tgatctcggt ggatgggcgt atggtcctta gccagcgcgc gatctccggg
      781 gcgaaccagt ttgatacgcg cgcgctcaca cccggcgcat actccctccg ggttctgtcc
      841 gcgagcggca ccgtgcgaca cgcgcgcttc gtgaaacgct gagtctccat cggctccagc
      901 taccttgggc accgaagatc tcttcggtgc ccaagcgctt tctggtcata caaaccgcct
      961 tcctcggtga tgccgtgctc gcgacagcat tgctggaaaa actgcatgcc ttcttcccgg
     1021 atgccgcggt cgacctggtg gtccgtaagg gcaatgacgg actgttcgat ggtcatcctt
     1081 tccttcggaa ggtcttcgtt tgggacaaac gcaccaacaa aacgcgcaac cttttcaagc
     1141 tgatcggcgc gttgcgcgaa gagcgctatg atcatgtgat caactgccag cgcttcttca
     1201 gcacgggcct gatgacggta ctcgcacgtg cagatgagaa gatcggctac gacaagaacc
     1261 cgctgagctt cctggtctcg cgaaagctga aacatgtgat cggtgatgga
//
LOCUS       DAOQOC010001038         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_91313_length_1309_cov_3.223285, whole genome shotgun sequence.
ACCESSION   DAOQOC010001038 DAOQOC010000000
VERSION     DAOQOC010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91313_length_1309_cov_3.223285"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PL106_14200"
     CDS             <1..718
                     /locus_tag="PL106_14200"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNA34274.1"
                     /translation="DGLEGFSYCLIKFDGVTNAALGDPKGYGRIEYAYHLMAVACGIN
                     MMPSTLLEERGRAHFLTQRFDRVQHAKTGETVRLHMATLCGVAHMDFNDPVRYSYEEA
                     FAVMRLLGLAYPDAVEFFRRMCFNVVARNCDDHTKNTSFLMDPQGEWHLSPAYDITFA
                     YNPANLWLKQHQMSVCGKRSDITRDDLLAVAKEMNIKKADAIIDEVVAGVKKWKTFAK
                     KAEMDVRQTEVIKGMLRTRI"
     gene            785..>1309
                     /locus_tag="PL106_14205"
     CDS             785..>1309
                     /locus_tag="PL106_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNA34275.1"
                     /translation="MRSRPTVDHPLVIMLSACLGGVGCGVDGGTNGDHASLRSWLTRP
                     EVRLVMFCPEDFSFGTPRMTPDNHGGNGFDVLDGKARSVAEDGTDWTTGMVKAALAMR
                     DHALREQVDLAILMDISGACGSTVTYLGPRFAKHKLYQQGPGVAAAALIRAGIPVISQ
                     RDDRSLRRDANLARR"
BASE COUNT          247 a          406 c          430 g          226 t
ORIGIN      
        1 cgacgggctg gagggctttt cctactgcct gatcaagttc gacggtgtga ccaatgcggc
       61 cttgggcgat ccgaagggtt acgggcgcat cgagtacgcc taccacctga tggccgtggc
      121 ctgtggtatc aatatgatgc ccagcaccct gctggaggaa cggggccgcg cgcacttcct
      181 cacgcaacgc ttcgaccgcg tgcaacatgc gaagaccggc gaaacggtgc gcctgcacat
      241 ggccacgctc tgcggggtgg cccacatgga cttcaatgac ccggttcgct acagctacga
      301 ggaggccttc gcggtgatgc gcctgctggg cctggcctac ccagatgccg tggagttctt
      361 ccgccggatg tgcttcaacg tggtggcccg caattgcgac gaccacacca agaacacctc
      421 cttcctgatg gacccgcaag gcgagtggca cctcagtccc gcctacgaca tcaccttcgc
      481 gtacaacccc gcgaacctct ggctgaagca gcaccagatg agcgtgtgcg gcaagcgttc
      541 ggacatcacc cgcgacgacc tgctggccgt ggccaaggag atgaatatca agaaggccga
      601 tgcgatcatc gatgaagtgg tggccggggt gaagaagtgg aagaccttcg cgaagaaggc
      661 ggagatggac gtgaggcaga ccgaggtgat caaggggatg ctgcgcacgc ggatctgaaa
      721 gcgaagagca caactacctt gcggcatgga gcggcccgct caccttcccc cttcggactt
      781 tttcgtgcgc tcgcggccca cggtggacca cccgctggtg atcatgctga gcgcttgctt
      841 gggcggagtg ggctgcggtg ttgatggtgg caccaatggt gatcacgcat cgctccgtag
      901 ctggctgacc cggcccgagg tgcgcttggt gatgttctgc cccgaggact tttccttcgg
      961 aacgccgcgc atgacgcccg acaaccatgg cggcaacggc tttgatgtgc tggacggcaa
     1021 ggcacggtcc gtggccgagg acggcaccga ctggaccacc ggcatggtga aggccgccct
     1081 cgccatgcgc gaccatgcct tgcgtgaaca ggtggacctg gcgatcctga tggacatcag
     1141 cggggcctgc ggcagcacgg tgacctacct gggcccacgc ttcgcgaagc acaagctgta
     1201 ccaacaaggc ccgggcgtgg cggctgcagc gttgatccgt gcgggcatcc cggtgatctc
     1261 gcagcgcgac gatcgctcgc tgcgacgaga cgcgaacctt gcgcgacga
//
LOCUS       DAOQOC010001039         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_91586_length_1307_cov_3.227636, whole genome shotgun sequence.
ACCESSION   DAOQOC010001039 DAOQOC010000000
VERSION     DAOQOC010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91586_length_1307_cov_3.227636"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PL106_14210"
     CDS             complement(<1..984)
                     /locus_tag="PL106_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34276.1"
                     /translation="MNGRTTPQTFHIPVMGLGYTIDTPLKVAHLGISSVVSIIEDELV
                     ERMREYHCRQSGETYEPIPKPGPDHRARRVTAYLDLLQRLVDRNVERVRATPITPDSE
                     SARYFELLPGDSFLYQRYKKMLDMGPGPERDGIDAELRDAMVPGSIDVNIMAKIDPVN
                     YDRDGNPLPPELCDAMASFRGFANSSLHSSLVLSAGYNPRLYAYIPEFPDFLPNEQGQ
                     LRKRLILKVSDLRSAQVQGRLLAKKGVWVSEFRIESGLNCGGHAFATDGLLMGPILDS
                     FRAEREALAMELWDACNTVLAASGKPVFTERPVQRITAQGGIGTAAEDRFLR"
     gene            complement(1097..>1307)
                     /locus_tag="PL106_14215"
     CDS             complement(1097..>1307)
                     /locus_tag="PL106_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34277.1"
                     /translation="PMVYVGVHVVYDVKGDPADEQAVLNVVSRSQNEICGVSAMLKCS
                     MPVTWELVYNGRVVHVQQGSIASPA"
BASE COUNT          229 a          390 c          424 g          264 t
ORIGIN      
        1 gcgcaagaac cggtcctcgg ccgcggtacc gatgccgcct tgtgccgtga tgcgttggac
       61 cggacgctcc gtgaagaccg gcttgcccga tgccgccaga acggtattgc acgcgtccca
      121 aagctccatg gccagcgcct cgcgttccgc gcggaacgag tcgaggatgg gacccatgag
      181 cagcccgtcg gtggcgaatg cgtggccgcc gcagttcaag ccggattcga tgcggaattc
      241 cgatacccag accccctttt tggccagcag gcggccctgt acctgcgctg aacgcaggtc
      301 gctcaccttg aggatgaggc gctttcgcaa ctggccctgc tcgttgggca ggaaatcggg
      361 gaattcaggg atgtaggcgt acaaccgcgg gttgtagccg gcgctgagca ccagggaact
      421 gtgcagggag ctgttcgcga atccccggaa ggaggccatg gcatcgcaga gttccggggg
      481 caaggggttc ccgtcccggt cgtagttcac cgggtcgatc ttggccatga tgttcacatc
      541 aatggaaccc ggtaccatcg catcgcgcag ttccgcatca atgccgtccc gttccggccc
      601 gggccccatg tccagcattt tcttgtaccg ctggtacagg aaggaatcgc ccggcaacag
      661 ttcgaagtat cgcgcacttt ccgaatccgg ggtgatggga gtggcccgca cccgttcgac
      721 gttccggtca accaagcgtt gaaggaggtc caggtacgcg gttacccgcc gggcacgatg
      781 gtcggggccg ggcttgggga tcggctcata ggtctcaccg ctttgtcggc agtggtactc
      841 acgcatccgc tcgaccagtt catcctcgat gatggagaca acggagctga tgcccagatg
      901 cgctaccttg agcggggtat cgatcgtata gcccagtccc atcacgggga tgtggaacgt
      961 ctgtggcgtt gttcgaccgt tcatggttcg tggcgatggg cctttgttga tgcgttcccg
     1021 atgatgatac cctgtgatcg ggaggtgatc ggtggttgca tggtcacgcc tcggaacggt
     1081 gtcggggtct ccccgttcag gcgggtgagg cgatggaacc ctgttgcacg tggacgacgc
     1141 gcccattgta taccagctcc caggtcacgg gcatgctgca cttgagcatg gcgctcacgc
     1201 cgcagatctc gttctgggag cgtgatacca cgttgagtac ggcctgttca tcggcggggt
     1261 cgcccttgac gtcgtacacc acatgcacac ccacatagac catgggc
//
LOCUS       DAOQOC010001040         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_91764_length_1306_cov_2.605116, whole genome shotgun sequence.
ACCESSION   DAOQOC010001040 DAOQOC010000000
VERSION     DAOQOC010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91764_length_1306_cov_2.605116"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1306
                     /gene="ppsA"
                     /locus_tag="PL106_14220"
     CDS             <1..>1306
                     /gene="ppsA"
                     /locus_tag="PL106_14220"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNA34278.1"
                     /translation="LFIVQARPETIHSRKATDRVVEYRLDAASMSGGASPEVIAKGVA
                     IGDKVGAGKVRILFSLDGRGGGTDGKDFQQGDVLVTDMTDPDWEPIMKKASAIITNKG
                     GRTCHAAIVAREMGVPAIVGCGNATDLLDTGMEITASCCEGDTGVIYKGIVPFTREET
                     MLNDLPEVRTPIMLNVASPDLAFKFAALPNAGVGLAREEFLINNYIQAHPLALLQHKE
                     LGDVALSGKISYLINGYEDEETFFVKRLSYGIAKIAAAFHPNKVIVRFSDFKSNEYKN
                     LLGGEHFEPDEENPMIGWRGASRYYSDAYKEAFGLECKALRRVREKMGLKNVVVMVPF
                     CRTVEELKKVMAVMKEYGPERGKEGLELYLMAEIPSNIILAEEFAQYIDGFSIGSNDL
                     TQLTLGLDRDSALVSHIYDERNEAVKGMIRSLISSAKRTGTKV"
BASE COUNT          278 a          417 c          412 g          199 t
ORIGIN      
        1 agctcttcat cgtgcaggcc cggcccgaga cgatccacag ccgcaaagcc accgatcgcg
       61 tggtggagta ccggctggac gccgcatcca tgtccggagg cgcatcgccc gaggtgatcg
      121 ccaagggtgt ggccatcggc gacaaggtgg gtgctggcaa ggtgcgcatc ctcttcagcc
      181 tggacgggcg gggtggcggt accgacggca aggacttcca acagggtgac gtgctggtga
      241 ccgacatgac cgatcccgat tgggagccga tcatgaagaa ggccagcgca atcatcacca
      301 acaagggcgg ccgcacctgc catgccgcca tcgtggcccg cgaaatgggc gtgccggcca
      361 tcgtggggtg cggcaacgca accgacctgc tcgataccgg gatggagata acggccagct
      421 gctgcgaggg cgataccggc gtgatctaca aggggatcgt ccccttcacg cgcgaggaga
      481 ccatgctgaa cgacctgccc gaggtgcgca cgccgatcat gctcaacgtg gccagccccg
      541 acctggcctt caagttcgcc gccctgccga atgccggtgt gggcctggcc cgcgaggaat
      601 tcctcatcaa caactacatc caggcccacc ccctggccct cctgcagcac aaggaactgg
      661 gcgatgtggc cctcagcggc aagatcagct acctgatcaa cgggtacgag gacgaggaga
      721 ccttcttcgt caagcgcctc agctacggca tcgccaagat cgccgccgcc ttccacccca
      781 acaaggtgat cgtgcgcttc agcgatttca agagcaacga gtacaagaac ctcctgggcg
      841 gcgagcattt cgaacccgat gaggagaacc ccatgatcgg ctggcgcggc gcatcgcgct
      901 actacagcga tgcgtacaag gaggccttcg ggctggagtg caaggccctg cgccgcgtgc
      961 gcgagaagat ggggctgaag aacgtggtgg tgatggtgcc cttctgccga acggtggagg
     1021 aactcaaaaa ggtaatggcg gtgatgaagg aatacggtcc ggagcgcggc aaggaaggcc
     1081 tcgaactcta cctgatggcc gagatcccca gcaacatcat cctggccgag gagttcgccc
     1141 agtacatcga cggcttctcc atcggcagca acgaccttac ccagctcacc ctcgggctcg
     1201 atcgcgacag cgcgctcgtg agccacatct acgacgagcg caacgaggcc gtgaagggca
     1261 tgatccgttc gctcatcagc tcggccaaac gcaccggcac caaggt
//
LOCUS       DAOQOC010001041         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_91927_length_1305_cov_2.423200, whole genome shotgun sequence.
ACCESSION   DAOQOC010001041 DAOQOC010000000
VERSION     DAOQOC010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91927_length_1305_cov_2.423200"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PL106_14225"
     CDS             <1..750
                     /locus_tag="PL106_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNA34279.1"
                     /translation="LHPFLVVASAGTTDTGTVDPIDAIADVAAKHGLWYHVDAAYGGF
                     FILTSKKNLFKGIERADSLIIDPHKGLFLPYGVGAVLIKDSAAVLHSHYYTANYMQDG
                     HNEELLKSPANLSPELTKHFRGLRVWLPLQLHGIAPFKACLEEKLLLVQYFRMKLGTL
                     GFHTGPEPDLSVSYFWYPFASRADELNKRLMDEIHKDGAVFLSSSVINGRFVIRMAIL
                     AFRTKKETIDEAVEMIQRCLDRVVQATGDAA"
     gene            747..1271
                     /locus_tag="PL106_14230"
     CDS             747..1271
                     /locus_tag="PL106_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA34280.1"
                     /translation="MKRLIILSDMWGAAKSDWPGHYIGKLRGQFEIAYYDSRTLANIS
                     DHIQNEEALHDLFVNGGADRAVVELLKLETEAEFILGFSIGGWIAWKACLAGLNVQDL
                     VAVSATRLRHETRRPSARISLFYGSLDPFIPGDAWFVAMGVRPILYRGESHGMYEQER
                     IASDVCGHLVEQRR"
BASE COUNT          268 a          376 c          410 g          251 t
ORIGIN      
        1 ctgcatccct tcctggtcgt ggccagcgcc ggcaccacgg ataccggcac cgtggatccg
       61 atcgacgcga tcgccgatgt ggccgcgaag catggcctct ggtaccacgt ggacgccgct
      121 tacggcggtt tcttcatcct cacatcgaaa aaaaacctgt tcaaagggat cgaacgcgcg
      181 gattcactga tcatcgatcc gcacaagggc ctcttcctgc cgtatggcgt gggcgccgtg
      241 ctgatcaagg acagcgcggc ggtgctccat tcccactact acacggcgaa ctacatgcag
      301 gacgggcaca acgaggagtt gctgaaaagc ccggccaacc tatctccgga attgaccaaa
      361 cacttccgcg gtctgagggt atggctgccg ctgcaattgc acggcatcgc gcctttcaag
      421 gcctgcctgg aggagaaact cctgctggtg cagtatttca ggatgaagtt ggggacgctc
      481 ggtttccaca caggacccga acccgacctc tccgtcagct atttctggta ccccttcgct
      541 tcgcgcgcgg atgaactgaa caaacggctg atggacgaga tccacaagga cggcgcggtc
      601 ttcctgagtt cctcggtgat caacgggcgc ttcgtgatcc gcatggccat ccttgcgttc
      661 cgaacaaaga aggagaccat cgatgaggca gtggagatga tccagcgctg cctggaccgt
      721 gtggtgcaag ccacaggcga tgcggcatga aaaggctgat catcctctcg gacatgtggg
      781 gcgcggcgaa gtcggattgg cccggtcatt acatcggtaa gttgaggggg caattcgaga
      841 tcgcgtacta cgacagcaga acgctggcga acatctcgga ccacattcag aacgaggaag
      901 ccctgcacga cctgttcgtg aacggcggtg cggatcgggc ggtagtggaa ctgttgaagc
      961 ttgaaacgga ggccgaattc atcctcggct tcagcatcgg tgggtggatc gcgtggaagg
     1021 catgcctggc ggggctgaac gtgcaggacc tcgtcgcggt atcggccacc cgcctgcgcc
     1081 acgagacccg cagaccatcg gcgcggatca gcttgttcta tggcagcttg gatcctttca
     1141 tccccggcga tgcctggttc gtggccatgg gtgtgaggcc cattctgtac aggggagaaa
     1201 gccacgggat gtacgagcaa gagcggatcg cttcggacgt ttgcgggcac ctcgttgagc
     1261 agcggcgata ggtcgcgaca tcccagtggg caccattcgt gggag
//
LOCUS       DAOQOC010001042         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92142_length_1303_cov_4.980769, whole genome shotgun sequence.
ACCESSION   DAOQOC010001042 DAOQOC010000000
VERSION     DAOQOC010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92142_length_1303_cov_4.980769"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..649
                     /locus_tag="PL106_14235"
     CDS             8..649
                     /locus_tag="PL106_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNA34281.1"
                     /translation="MRNTISRTTGLTLSALVLFTVTTASAQTQINPQLGLTYQNLTGK
                     LVQGQDFKGSVGYTLGADLRFGNRLFFQPGVFLSRNRTISSYDGGNVIESESKLITTN
                     LKLRALVGYRIIDSYQFDLRLVAGPSYDVLMSVDGKDNNGIAWNKGDFSTGSFNVEAG
                     LGFDMGMFTLAPMASFGLNRAFSDAASVKDIDSRYITYGLSIGVNFGDDDKND"
     gene            complement(714..>1303)
                     /locus_tag="PL106_14240"
     CDS             complement(714..>1303)
                     /locus_tag="PL106_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34282.1"
                     /translation="VLACWSMSGAIGDTSWIQPGDQPLCSMHETGDNVVPYGTQEVSV
                     VGIPTGLIASGSGDMYTRMVNIGVPDCFRSYDQADHVGYLNYDNEGAMNYLSGFLANV
                     VCGAEDVCSDLPTALAELEMRDLIPVPNPVATSFFFNTDERVDVRVLDLAGRVLLSQK
                     AAPGRVLIDVSTLPSGIYVVRASGRSVRTARIIKD"
BASE COUNT          284 a          410 c          376 g          233 t
ORIGIN      
        1 cacgatcatg agaaacacca tctctcgcac gaccggactc acactgagcg cgctcgtgct
       61 tttcacggtc accacggcaa gcgcacagac gcagatcaac ccgcaactgg gcctcacgta
      121 ccagaacctg accggcaagt tggtacaggg ccaggacttc aaagggtccg tcgggtatac
      181 gctgggcgcc gacctgcgct tcggcaaccg gctcttcttc cagccgggcg tcttcctgag
      241 ccgcaatcgc acgatcagca gctacgacgg tggcaatgtg atcgagagtg aaagcaaatt
      301 gatcaccacc aacctgaagt tgcgggccct ggtgggctac cggatcatcg acagctacca
      361 gttcgacctg cggctggtcg cgggccccag ctacgatgtg ctgatgagcg tggacggcaa
      421 ggacaacaac ggcatcgcct ggaacaaggg cgacttcagc accggctcgt tcaacgtgga
      481 ggccggtctc ggcttcgaca tgggcatgtt cacgctggcc cccatggcga gcttcggcct
      541 caaccgggca ttcagcgatg cggcgagcgt gaaggacatc gacagcaggt acatcaccta
      601 tggcctcagc atcggcgtca atttcggcga cgatgacaag aacgactgag gatcccttta
      661 acaggaaggc cctcccggcg ttcgggaggg ccttcctgtt atcaagtaca gcttcagtcc
      721 ttgatgatgc gggcggtccg cacggaacgt ccggaagcgc gcacgacgta tatcccggat
      781 ggcaacgtgg atacatcgat caggacccgg cccggtgcgg ccttctggct gagcagtaca
      841 cggccagcaa gatccagcac acgcacatcc acccgctcgt ccgtgttgaa gaagaatgag
      901 gtcgcaacag gattcggaac cgggatcaga tcccgcattt ccaactcggc aagtgcggta
      961 ggcaggtccg agcagacatc ttccgcgcca cagaccacat tggccaggaa tccggagaga
     1021 taattcatcg cgccttcgtt atcgtagttg aggtagccca cgtgatccgc ctggtcgtaa
     1081 ctgcggaaac aatcgggcac cccgatgttc accatgcgcg tgtacatatc accgcttccc
     1141 gaagcgatca ggccagtggg gatcccgacc acgctcacct cctgcgtacc gtacggcaca
     1201 acattgtcac cggtctcgtg catgctgcac aggggctgat ccccgggttg gatccagctg
     1261 gtgtccccga tggcaccgct catgctccag caggccagta cat
//
LOCUS       DAOQOC010001043         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92195_length_1303_cov_2.989583, whole genome shotgun sequence.
ACCESSION   DAOQOC010001043 DAOQOC010000000
VERSION     DAOQOC010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92195_length_1303_cov_2.989583"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PL106_14245"
     CDS             <1..697
                     /locus_tag="PL106_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNA34283.1"
                     /translation="ELKPSSDDAGLLAAVLEVLRLLAPDLAAQATIVEGRLAGVDALI
                     ATGSDNTARYFEHYFGHLPRIVRKNRTSVAVLDGSETEEELLALGEDLFRYFGLGCRN
                     VGKLHIPHDFDLDRLFKAIFKWGHLAKHNKWANNYDYHKAIWLMDRVPLLENGFVLLK
                     EDAGLHSPLGSIYYERYTDRSHVTTSLAKFESKLQCVVGHGHLAFGHAQCPGLQDYAD
                     GVDTMRFLVELGR"
     gene            complement(825..>1303)
                     /locus_tag="PL106_14250"
     CDS             complement(825..>1303)
                     /locus_tag="PL106_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34284.1"
                     /translation="FDQITIANYWSFDIRNDPAFLLTNKFVDATGATQVRTIQAGSTF
                     KNGIIHGSNGNEFKLDLDESTDPQFMFSYFLFRTDQPTNDASHFDQATTYRNLDPGFV
                     DGPAGDLHLKANSSARGKADPVVSLFLDLDGEPRPNTTFGPEDNFKPDLGCYEYRE"
BASE COUNT          252 a          353 c          420 g          278 t
ORIGIN      
        1 cgaattgaaa cccagcagcg atgatgccgg tcttcttgcg gcagtgctcg aggtgttgcg
       61 tttgctggca cccgatctgg ccgcccaggc cacgatcgtc gagggccggt tggcgggggt
      121 ggatgcgttg atcgctacgg gcagcgacaa taccgcacgg tacttcgagc actacttcgg
      181 gcatttgccg cgcatcgtcc ggaagaatcg caccagtgtg gcggtacttg acggttccga
      241 aacggaggag gaattgctgg cgttggggga ggacctgttc cgctacttcg gcctcggttg
      301 ccgcaacgtg ggcaaactcc acattccgca cgacttcgat ctggaccggc tcttcaaggc
      361 cattttcaag tggggtcacc ttgccaagca caacaaatgg gcgaacaact acgactacca
      421 taaggccatc tggcttatgg accgggttcc gctcctcgag aatggctttg tgctgcttaa
      481 ggaggatgcg ggcctgcaca gcccgttggg atcgatctac tacgagcggt acaccgatcg
      541 ctcgcacgtg accacttcgc tggccaaatt cgaaagcaag ttgcaatgcg tggtgggaca
      601 tggccatctg gctttcgggc acgcgcaatg cccgggcctt caggactacg cggatggggt
      661 ggacacgatg cgcttcctgg tcgagttggg tcgttgaacc ccgctccatc ggttcactga
      721 ggcacaagga cgccgaggat gctcagggaa acccgcacgc acgaatgtga tgaggtacga
      781 gctgcggagt attcgttggc agcgtatcgg atcgtgaaag ggggctattc gcggtactcg
      841 taacacccga ggtccggttt gaagttgtcc tcaggtccga aggtggtgtt cgggcgcggc
      901 tcaccatcca gatccaggaa caggcttacc accgggtccg ctttgccccg tgcgctcgaa
      961 ttcgccttca ggtggagatc accggccggg ccatccacga agccgggatc caagttccgg
     1021 tacgtggtgg cctggtcgaa atgcgaggca tcgttggtgg gctggtcggt gcggaacagg
     1081 aaataactga acatgaactg cggatccgtg ctctcgtcca ggtcgagctt gaactcgttg
     1141 ccgttggagc cgtgaataat accattcttg aaggtgctcc ccgcctggat ggtgcgtacc
     1201 tgcgtggcgc cggtcgcatc cacgaatttg ttcgtgagca gaaaggcggg atcgttgcgg
     1261 atgtcgaagc tccagtagtt ggcgatggtg atctggtcaa att
//
LOCUS       DAOQOC010001044         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92365_length_1302_cov_2.752205, whole genome shotgun sequence.
ACCESSION   DAOQOC010001044 DAOQOC010000000
VERSION     DAOQOC010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92365_length_1302_cov_2.752205"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PL106_14255"
     CDS             complement(<1..900)
                     /locus_tag="PL106_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34285.1"
                     /translation="MRWFIVAIGSLLFLVVVIIVLLLYPVVQTWIAGIVGRKLSDDLG
                     ITVRIERVELRPFALNRLHGVYIADLRGDTLIAVDELRIRGLKLDTEAHTVKARQVEL
                     YDARFKLAKAPGDVHSNLTNLLEKLASSDTTTAPTPWTVDCAAFDIQRFHFSFNDANE
                     VREPFGVDFDHVDVNTADVRGTDLRVAGDSIMAFLDRVRLHERSGLVVDELSGHTLVS
                     PRGIIIDGMRLCTPGSWLHGDLKFTTDSWLDYNDFINKVRMRLALQESRLNFEDIAWF
                     APDLEGIDLRIGVSGNYRGTVSEL"
     gene            1030..>1302
                     /locus_tag="PL106_14260"
     CDS             1030..>1302
                     /locus_tag="PL106_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNA34286.1"
                     /translation="MTRSAPLILGIESSCDETAAAVMAGGVVRSNVVASQEVHAKWGG
                     VVPELASRAHQEHIVPVVREALEQAGVRKEDLDGIAVTRGPGLIGAL"
BASE COUNT          248 a          413 c          404 g          237 t
ORIGIN      
        1 caattcgctc accgtaccac ggtagttccc actgacgccg atccgcaggt cgatgccctc
       61 caggtccggc gcgaaccagg cgatatcctc gaagttcagc cgggactcct gaagtgcgag
      121 gcgcatgcgc accttgttga tgaagtcgtt gtagtcgagc cagctgtcgg tcgtgaactt
      181 caggtcgccg tggagccagg accctggcgt gcagaggcgc atgccatcga tgatgatgcc
      241 gcgtggactc accaacgtgt ggccgctgag ttcatccacc accagaccgc tgcgttcgtg
      301 caaccgcacc cggtccagaa aggccatgat cgaatccccg gccacacgaa ggtccgttcc
      361 ccggacatcg gccgtgttca catctacatg atcgaagtcc acgccgaacg gttcacgtac
      421 ttcgttcgca tcattgaagc tgaagtggaa ccgttggata tcgaacgcgg cgcagtccac
      481 ggtccatggg gtcggggcgg tggtggtatc cgagctggcc agtttctcca ggaggttggt
      541 caggttgctg tggacatcgc cgggggcctt cgcgagtttg aatcgtgcgt cgtacagctc
      601 cacctgccgg gccttcactg tatgggcctc agtatcgagt ttcagcccgc ggatgcgcaa
      661 ctcgtccacc gcgatcagtg tatcgccacg cagatcggcg atgtacacgc catgaagccg
      721 gttcagcgcg aagggccgca attcgacgcg ctcaatgcgc acggtgatgc ccaggtcatc
      781 gctcaacttg cggcctacga tgccggcgat ccacgtttgc accaccggat acaggaggag
      841 cacgatgatg accaccacca ggaacaacag ggagccgatg gccacgatga accatcgcac
      901 gatgcgtttc cagcgcttcc gtatgggctt ctcccctgcc agcttcctgt agtttcggcc
      961 cgcgtaccca gccaactatc ggaccaacgt gcaaaggtac ccggcgccct catgcgggcg
     1021 cccatgacca tgacccgctc cgcccccctc atcctcggga tcgaaagctc gtgcgacgaa
     1081 acggccgcgg ccgtgatggc cggaggcgtg gtgcgaagca acgttgtggc ctcgcaggag
     1141 gtgcatgcca agtggggtgg tgtggtacct gaactggcca gccgcgcaca ccaggaacac
     1201 atcgtgcccg tggtgcgcga ggcacttgaa caagcgggcg tgcgcaagga ggacctcgat
     1261 ggtatcgccg taacgcgcgg gccggggctg atcggggccc tg
//
LOCUS       DAOQOC010001045         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92374_length_1302_cov_2.690457, whole genome shotgun sequence.
ACCESSION   DAOQOC010001045 DAOQOC010000000
VERSION     DAOQOC010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92374_length_1302_cov_2.690457"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PL106_14265"
     CDS             complement(<1..918)
                     /locus_tag="PL106_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34287.1"
                     /translation="MTIDWKKLLPAGAAVALFIALTLAYFSPVMEGKRLIQGDIRNHA
                     GMAQDIVEHRAAYGEEPLWAGSMFSGMPAYQISVIWSGSALHGLDRLFHGFLPRPADF
                     LFLYLIGMFIGLRCMRVDPWLSIVGAIACAFSSYFFVILEAGHNSKANAIGYMPMVLG
                     ALYMLLRGNKLLGAALFALFLSLLVYVNHVQVAYYLGMLLLLFGLAEAWRAISEKRIP
                     DFAQRAGLALVGVALALLTNAGMLWSTYEYGKFTTRGPSELTITPEGDSAHANMTGGL
                     DRDYVTNWSYGKQESFTLIVPDAKGGASGS"
     gene            complement(929..1165)
                     /locus_tag="PL106_14270"
     CDS             complement(929..1165)
                     /locus_tag="PL106_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34288.1"
                     /translation="MFLLSANGTLRTILVLLIVWLLLRAWVRSRQPVQGRPPGTHWSA
                     PDNRAKGEVRIERTESAPHARQGPVEDADFEEIK"
BASE COUNT          239 a          397 c          439 g          227 t
ORIGIN      
        1 cgaaccactg gcacctccct tggcatcggg cacgatgagt gtgaagctct cctgtttgcc
       61 gtagctccaa ttggtcacgt agtcgcgatc cagacctccg gtcatgttgg catgggcgct
      121 gtcgccttcc ggggtgatcg tgagctcgct gggtccccgc gtggtgaact tgccgtactc
      181 gtatgtgctc cagagcatgc ccgcgttggt gagcagggcc agggccacgc caaccagggc
      241 cagtcccgcg cgctgcgcga agtcagggat ccgtttctcg ctgatcgcgc gccaggcctc
      301 ggccagtccg aagagcagca gcagcatgcc caggtagtag gccacttgca cgtggttgac
      361 gtacacgagc agcgagagaa agagtgcgaa gagtgccgct ccgaggagtt tgttcccgcg
      421 cagcagcatg tagagcgcgc cgagcaccat gggcatatag ccgatggcgt tggccttact
      481 gttgtggccg gcctccagga tcacgaagaa gtagctgctg aatgcgcagg cgatcgcgcc
      541 cacgatgctc agccagggat caacgcgcat acatcggagg ccgatgaaca taccgatgag
      601 gtagaggaag aggaagtcgg ccggtcgcgg aaggaagccg tggaagaggc gatcgagccc
      661 atgcagcgcg ctgcccgacc agatcacact gatctggtag gccggcatgc cgctgaacat
      721 gctgccggcc cagagcggct cctcgccgta cgccgcgcga tgttccacga tgtcctgtgc
      781 catgcccgcg tggttgcgga tatcgccttg gatcagccgc ttgccttcca tcaccggact
      841 gaaataggcc agggtgagcg cgatgaacag ggcaacggcc gcgcccgcgg gcagcagctt
      901 cttccagtcg atggtcatac gggaatggtt acttgatctc ttcgaagtcg gcatcctcca
      961 cggggccctg tcgcgcatgc ggcgcacttt cggtgcgttc gatgcgtacc tcgcctttcg
     1021 cgcggttgtc cggcgcgctc cagtgggtac cgggcggtcg gccctgcacc ggttggcggc
     1081 tgcgcaccca ggcacgcagg agcagccaca cgatcaggag gaccaggatc gtacgcaggg
     1141 ttccgttggc gctcagcagg aacatggcgg gcgaaggtag gcgcgtgatg aaaagcacca
     1201 tttccacgcg tccttgtggg gagccagccg gaacggggtg ctcccgggcg ccaggcggag
     1261 tacccggtga acagggagca cgcttgggat catgacccgc ac
//
LOCUS       DAOQOC010001046         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92428_length_1302_cov_2.056937, whole genome shotgun sequence.
ACCESSION   DAOQOC010001046 DAOQOC010000000
VERSION     DAOQOC010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92428_length_1302_cov_2.056937"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1188
                     /locus_tag="PL106_14275"
     CDS             19..1188
                     /locus_tag="PL106_14275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNA34289.1"
                     /translation="MHLSPLVTSLVEPATLLMARRSRELKAQGRDIIDLSLGEPDHDP
                     PTFALAAAHEALNGPWHKYPPVNGHLDVRQAIARKFKRDNGLEYTADQIVVSTGAKQS
                     LINVVMALVGPGDEVVIPAPYWVTYSEQVRLAGGTPVIVTSTLEENWKPPVERIAAAI
                     TPRTRLLMFSSPCNPSGSVLTLEEMEALAAVVRKHPELYVVSDEIYEHIVFSGTHRSF
                     AALPGMMERTVTVNGLSKAFALTGWRMGYIGAPLWIAQAATKIQGQFTSGANSIAQRV
                     ALACVEADPAQLGGMRADFLRRRDLLVEALKAVPGWRCNVPQGAFYVLPDASASLRKL
                     GLKTSQDLCMHLLEHAGVGVVEGDAFGATGTVRISYATSDDKLLAAVDRIAQALV"
BASE COUNT          221 a          450 c          430 g          201 t
ORIGIN      
        1 acgcgcaggc ccccacgcat gcacctttcc ccgctggtca catccctggt tgaacccgca
       61 acgctcctca tggcccgccg cagccgcgag ctgaaggccc agggccgcga catcatcgat
      121 ctgagcctgg gcgaaccgga ccacgacccg cccaccttcg ccctggccgc cgcgcatgag
      181 gccctcaacg gcccctggca caaatacccg ccggtgaacg gccacttgga cgtgcggcag
      241 gccatcgccc gcaagttcaa gcgcgacaac gggctggagt acaccgctga tcagatcgtg
      301 gtgagcactg gcgccaagca gtcgctcatc aacgtggtga tggccctggt gggccccggc
      361 gatgaggtgg tgatccccgc gccgtactgg gtcacttaca gcgagcaggt gcgcctggcc
      421 gggggcacgc ccgtcatcgt taccagcacc ctggaggaga actggaagcc gccggtggag
      481 cgcatcgccg cggccatcac cccgcgcacg cggctgctga tgttcagctc gccctgcaac
      541 ccgagcggct cggtgctcac gttggaagag atggaggcgc tggcggccgt ggtacgcaag
      601 cacccggagt tgtacgtggt gagcgacgag atctacgagc acatcgtgtt cagtggcacg
      661 caccgctcct tcgcggcgtt gccgggcatg atggaacgca ccgtcacggt gaacggactg
      721 agcaaggcct tcgccctcac gggctggcgc atgggctaca tcggtgcgcc attgtggatc
      781 gcgcaggccg ctaccaagat ccaaggacag ttcaccagcg gggccaacag catcgcccag
      841 cgggtggcac tcgcgtgcgt ggaggccgat cccgcacaac tcggcggcat gcgggccgac
      901 ttcctgcgcc gccgcgacct gcttgtggaa gccttgaagg ccgtgcccgg ctggcgctgc
      961 aacgtgccgc agggagcctt ctacgtgctt cccgacgcca gtgccagcct gaggaagctc
     1021 gggttgaaaa catcccagga cctctgtatg cacctgctgg agcacgcggg cgtgggcgtg
     1081 gtggagggcg atgccttcgg ggccaccggc accgtgcgca tcagttatgc caccagcgac
     1141 gacaagttgc tcgccgccgt ggaccgcatc gcccaagccc tggtctgatc cgtgagcacc
     1201 cccatcgacg cccgcaccat cgccgaaggc ttcggccgca ccacggcatt ggtggtgggc
     1261 gatgtgatga tggatcggtc gctgcgcggt tccgcggacc gt
//
LOCUS       DAOQOC010001047         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92496_length_1301_cov_3.520064, whole genome shotgun sequence.
ACCESSION   DAOQOC010001047 DAOQOC010000000
VERSION     DAOQOC010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92496_length_1301_cov_3.520064"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..>1301
                     /locus_tag="PL106_14280"
     CDS             183..>1301
                     /locus_tag="PL106_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HNA34290.1"
                     /translation="MINLIKAKTPSATKDTLVVLKDPTQLRGLELERNDRQYLVEQLN
                     GDANVATCDISGRLVLVHRIREGRQTAQLERARCAGNEMVQKLILAKRDEAQLISLQN
                     DTAITLALAEGAVLGAYEFRKYKTSGKNGSLKKLSILAPELSSKALGELVDVSEAVLT
                     ARDLVNEPNSFLTAVQLGKELWTLSRNAGFKLQTFDKSRIEALGMGGLLAVNKGSIDP
                     PTFNVLEWKPKNAVNRKPILLVGKGVVYDTGGLSLKPTPNSMDSMKCDMAGAAAVACA
                     ISAVAKQELPVHVIGLIPATDNRPGGNAYAPGDVIRMHSGLTVENLNSDAEGRIILAD
                     ALSYGEQFDAELVLTIATLTGAAMRAIGTYGTVTMGTAD"
BASE COUNT          279 a          404 c          409 g          209 t
ORIGIN      
        1 gaggatgctc atgagcagcg ggccgaagtt cgcacattcc cgggccgcac cgacgccgaa
       61 cgaggaacgg taggacgctt ggggccggtg gtggcccggg gagcatgagg cctgcgaccc
      121 ggtttctatc ttgccccgct tccgcactgc ttccattggc cccaagagcg gatcccgccc
      181 ccatgatcaa cctgataaaa gcgaaaaccc cgagtgcgac caaggacaca ctggtcgtgc
      241 tgaaggatcc gacccaactg cgcgggctcg aactggagcg gaacgaccgc cagtacctgg
      301 tggagcaact caatggcgat gccaacgtgg ccacgtgtga catcagcgga aggttggtgc
      361 tcgtgcatcg catccgggag ggtcggcaga ccgcccaact cgaaagggcc cgttgtgcgg
      421 gcaacgagat ggtccagaaa ctgatcctgg cgaagcgcga tgaggcccag ttgatcagcc
      481 tgcagaatga taccgcgatc acactcgccc ttgcggaagg ggccgtgctc ggggcgtacg
      541 agttccgcaa gtacaagacc tccggcaaga acggttcgtt gaaaaaactg tccatcctgg
      601 ctccggaact gagcagcaag gcactgggcg aattggtgga cgtgagcgaa gccgtgctca
      661 ccgcacgcga cctggtgaat gaacccaaca gcttcctcac cgccgtgcag ctcggcaagg
      721 aactatggac cttgtcaagg aacgcgggct tcaaactgca gaccttcgac aagagtcgta
      781 tcgaggcgct gggcatgggc ggtcttctgg ccgtgaacaa gggcagtatc gatcctccca
      841 ccttcaatgt gctggaatgg aagcccaaga acgcggtgaa caggaagccc atcctgctcg
      901 tgggcaaggg cgtggtatat gataccggcg gccttagcct gaagcccacg cccaacagca
      961 tggattccat gaagtgtgac atggccgggg ccgcagcggt ggcttgtgcg atctcggccg
     1021 tggccaagca ggaacttcct gtccacgtca tcggcctgat ccccgccacc gacaaccggc
     1081 ccggtgggaa cgcctacgcc cccggtgacg tgatccgcat gcacagcggg ctcaccgtgg
     1141 agaacctgaa cagcgatgcc gaaggacgga tcatccttgc ggacgcgctc agctatggcg
     1201 agcagttcga tgccgaactc gtcctcacca tcgccacgct caccggggcc gccatgcgcg
     1261 cgatcggcac ctacggcacc gtgaccatgg gtacggccga t
//
LOCUS       DAOQOC010001048         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92695_length_1300_cov_3.046586, whole genome shotgun sequence.
ACCESSION   DAOQOC010001048 DAOQOC010000000
VERSION     DAOQOC010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92695_length_1300_cov_3.046586"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PL106_14285"
     CDS             <1..627
                     /locus_tag="PL106_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34291.1"
                     /translation="GRTILFVSHNMTAIASLCSRVIWLHDGRVRMDGPTSDVVRAYLG
                     TYASHSNERRWVVGQDAPGNDLVRLTGVSIEPDATTDGEELLDVNTSFKIRIHYHNSG
                     ITDDDMNVTIQLYNAEDIMVFTSGWRQFGPHTGRVASGAGSVWCAIPAQLLNTGNHRI
                     VVNFFRNGKMMFQVEETIGFEVHEGERTGSWYGRLQGVVRPRLQWGTN"
     gene            722..>1300
                     /locus_tag="PL106_14290"
     CDS             722..>1300
                     /locus_tag="PL106_14290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA34292.1"
                     /translation="MEHEPIEHVKDPESITYWEEYYRANPHPFNPSAFAMEMAKRMGT
                     AGPVIELGCGNGRDAVFLGAQSKGPVVAIDQCEEETRRLNAEHGNKNLQFVAGDFSRY
                     TPTTAPRYIYSRWTMHAVDEVTEDRTLDWVAAALVPGGEFMVEARSIRDDLYGKGESL
                     GGHAFFTDHYRRFMDPERFAQKLRNRGLEIISL"
BASE COUNT          274 a          394 c          425 g          207 t
ORIGIN      
        1 ggccgcacca tcctattcgt cagccacaac atgacggcca tcgcttcgtt gtgctcgcgc
       61 gtgatctggc tgcacgacgg ccgtgtgcgc atggacggcc ccacaagcga tgtggtgcgc
      121 gcctacctgg gaacgtacgc cagccacagc aacgagcgcc gttgggtggt gggacaggac
      181 gcacccggca acgacctggt ccggctcacc ggcgtgagca tcgaaccgga cgcgaccacc
      241 gatggggaag aactgctcga cgtgaacaca tcgttcaaga tccgtattca ttaccacaat
      301 agcgggatca cggacgacga catgaacgtg acgatccagc tgtacaacgc ggaggacatc
      361 atggtcttca ccagcggctg gcggcaattc ggtccgcaca cggggcgggt ggcctcggga
      421 gcgggcagcg tgtggtgcgc catccctgcc caactcctga atacgggaaa ccaccgcatc
      481 gtggtgaatt tcttccggaa cgggaagatg atgttccaag tggaagagac gatcggattc
      541 gaggtgcatg aaggggagcg gaccggctcc tggtacggcc gcctccaggg cgtcgtgcgg
      601 ccccggttgc agtggggcac gaactgaggt gggagcgccc ccggtccgta ctttcgcagg
      661 ctccggccgt cccggccgcg ggcggatcat ccatacgaac ggaacacggg cgcaagagca
      721 catggaacac gaaccgatcg aacacgtgaa ggaccccgag agcatcactt actgggaaga
      781 gtattaccgc gcgaatccgc atccgttcaa ccccagtgcc ttcgccatgg aaatggccaa
      841 gcggatggga acagccggtc cagtgatcga actggggtgc ggcaacggtc gcgatgcggt
      901 tttcctgggt gcgcagtcca aaggcccggt ggtcgccatc gaccaatgcg aagaagagac
      961 ccgccgactc aacgccgaac acggcaacaa gaacctgcaa ttcgtggcag gcgacttctc
     1021 gcggtacacg cccaccacgg ccccgcgcta catctactcg cgctggacca tgcatgctgt
     1081 ggatgaggtg accgaggacc gcaccctgga ctgggtcgca gcggcattgg tgccgggcgg
     1141 cgagttcatg gtggaggcgc gcagcatccg cgacgacctg tacggcaagg gagagtcgct
     1201 gggcgggcat gcgttcttca cggaccacta tcggcggttc atggaccccg aacgtttcgc
     1261 acagaagctg cggaacaggg gattggagat catctcattg
//
LOCUS       DAOQOC010001049         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_92844_length_1299_cov_3.183280, whole genome shotgun sequence.
ACCESSION   DAOQOC010001049 DAOQOC010000000
VERSION     DAOQOC010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92844_length_1299_cov_3.183280"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..953)
                     /locus_tag="PL106_14295"
     CDS             complement(27..953)
                     /locus_tag="PL106_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNA34293.1"
                     /translation="MPIDNKEIQALSQLEFKARQVVEGFLAGLHKSPFHGFSVEFAEH
                     RIYNPGESTRHIDWKLYARTDKLFVKRYEEETNLRCQLVIDASSSMYYPQLKPGDSEG
                     LNKIRFSVYAAAALTQLMRKQRDAVGLTVFTDEVKVAEQARSNAVHLKHVMIHLENLL
                     AANAPAQGQKTSIVDALHDISNRAPRRSLVVILSDMLDGQDQAAMFDALQHLRFNKHD
                     VILFHVTDRRKELELELEDRPYTFVDLETGEQVKAHAAEIRDEYRTAVADHWHQLKLK
                     CGQYRIDLVEADINKGFSPILLEFLLKRARLY"
     gene            complement(1063..>1299)
                     /locus_tag="PL106_14300"
     CDS             complement(1063..>1299)
                     /locus_tag="PL106_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA34294.1"
                     /translation="QQRFKGPVSSVVEEIAKEYEGKAVVGKLNVDNNPQIATKYGIRS
                     IPTILFLKNGEVVDRSVGAVPKAALATKLQGQLA"
BASE COUNT          202 a          389 c          418 g          290 t
ORIGIN      
        1 ctcgcctctc tcctcacgcc gttccgtcag tacagccgcg cccttttcag caggaattcc
       61 agcaggatgg gggagaagcc cttgttgatg tcggcctcca cgaggtcgat gcggtactgg
      121 ccgcacttga gtttgagctg gtgccaatgg tcggccacgg cggtgcgata ctcatcgcgg
      181 atctcggcgg catgggcctt cacctgctca ccggtttcca ggtccacgaa ggtgtacggg
      241 cggtcctcca gttcgagttc cagttccttg cggcggtcgg tcacgtggaa gaggatcaca
      301 tcgtgtttgt tgaagcgcag gtgctggagg gcgtcgaaca tcgcggcctg gtcctgaccg
      361 tcgagcatgt cgctcaggat caccaccagc gagcggcgcg gcgcgcggtt actgatgtcg
      421 tgcagcgcgt ccacgatgga ggtcttctgg ccttgggcgg gagcgttcgc cgcgagcagg
      481 ttctccaggt ggatcattac atgcttcaga tgcacggcgt tgcttcgggc ctgctcggcc
      541 accttcactt catcagtgaa cacggtgagg cccacggcat cgcgctgctt gcgcatgagc
      601 tgggtgagcg cggcagcggc gtatacgctg aagcggatct tgttcagccc ctcggaatcg
      661 cccggcttca gctgcgggta gtacatactg ctgctggcgt cgatcaccaa ctggcaacgc
      721 aggttggtct cctcctcgta gcgcttcacg aacaacttgt ccgtgcgggc gtacagcttc
      781 cagtcgatgt ggcgcgtgct ttcgccgggg ttgtagatgc ggtgctcggc gaactccacg
      841 ctgaagccgt ggaaggggct tttgtgcagg ccggcaagga agccctcaac cacctggcgg
      901 gccttgaatt ccaactggct cagggcttgg atctccttgt tgtcgatggg catgttggaa
      961 gctgtcacgc tgagcccgcc gaagcgtcac tcatgctgct gcaaagaacg acaagccccc
     1021 cggttcgccg gagggcctgt tcgatcttgt tgtcagaatc gcctacgcga gctgcccttg
     1081 caatttggtg gccagggcgg ccttgggcac ggcgcccacg ctgcggtcca ccacttcgcc
     1141 gttcttcaga aagaggatgg tggggatgct gcggatgccg tatttggtgg cgatctgcgg
     1201 gttgttgtcc acgttgagct tgcccacaac agccttgcct tcgtactcct tggcgatctc
     1261 ttcgacgacg ctgctcaccg ggcccttgaa gcgctgctg
//
LOCUS       DAOQOC010001050         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_93266_length_1296_cov_3.130540, whole genome shotgun sequence.
ACCESSION   DAOQOC010001050 DAOQOC010000000
VERSION     DAOQOC010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93266_length_1296_cov_3.130540"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PL106_14305"
     CDS             complement(<1..971)
                     /locus_tag="PL106_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNA34295.1"
                     /translation="MKILVLRFSSIGDIVLTSPVLRCVKRQLPGAELHVATKRIFMDL
                     VRYSPYVDKAHDLGDDLGELIHRLKAERFDAVVDLHHNLRTWRVKRALGVPATSFAKL
                     NLEKWMLVNLRRDRMPRKHIVDRYMDAVAHLGVKNDNEGLDLFIHPDREVMTDELPES
                     HRAGFVALCVGGAHATKRLPVHKLVDLAGRIPGPITIIGGPDDKAVARAIANAVGGRA
                     WDATGRYDLLGSASLIRQARSVIAHDSGAMHIAAAFKKNTVSIWGSTVPLLGMGPYIP
                     TRPDRAHIMEVAGLDCRPCSKIGFEHCPKGHFRCMENQDTARIAEIAT"
     gene            1165..>1296
                     /locus_tag="PL106_14310"
     CDS             1165..>1296
                     /locus_tag="PL106_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNA34296.1"
                     /translation="MFIDTHAHLYHKQFDVDREEMLARAVDAGVGRFYLPNIDLESVA
                     "
BASE COUNT          245 a          416 c          394 g          241 t
ORIGIN      
        1 gtggcgatct ccgcgatgcg cgcggtatcc tggttctcca tgcagcgaaa atgccccttg
       61 ggacaatgct cgaaaccgat cttggaacag ggccggcaat cgaggccggc cacctccatg
      121 atatgcgcac gatccgggcg ggtcgggatg tacgggccca tgccgaggag cggcacggtg
      181 ctgccccaga tgctcacggt attttttttg aacgccgctg cgatgtgcat ggccccgctg
      241 tcatgcgcga tgacgctccg ggcctggcgg atgagcgatg cgctaccgag caggtcatac
      301 cggcccgtgg cgtcccaggc cctgccgccc accgcgttgg cgatcgcgcg ggcgacagct
      361 ttgtcatccg gtccaccgat gatggtgatg gggccgggga tacgcccggc gagatcgacc
      421 agtttatgga ccggcaaccg tttcgtggcg tgggccccgc cgacgcacag tgccacgaag
      481 ccagcgcggt ggctttccgg cagttcgtcc gtcattacct cacgatcggg atgaatgaac
      541 aggtccagcc cctcgttgtc gttcttcacc cccaggtgcg ccaccgcgtc catgtagcga
      601 tcaacgatgt gcttgcgggg catgcgatca cgccgcaggt tcaccagcat ccacttctcc
      661 agattcagtt tcgcgaagct ggtggcgggc acgcccagcg cgcgtttcac cctccaggta
      721 cgcaggttgt ggtgcaggtc gacaaccgca tcgaaacgct cagccttcaa gcgatggatg
      781 agttcgccga ggtcatcacc gagatcatgg gccttgtcca cgtacggact gtaacgcacc
      841 aggtccatga agatgcgctt ggtggccaca tgcagctccg ccccgggaag ctgacgcttc
      901 acacaacgca acacgggact cgtcagcacg atatccccga tggacgaaaa gcgcagaacg
      961 aggatcttca tgccggcatg ggaccaagtg gacctgtgga tcgggactcc acagcacggg
     1021 atggccgcgc ccaaaagtag ccgctgatgt ccccggtcaa cgatatccca tcgccgatat
     1081 tcaagccctc atgagcggac gcgcatccgg tcgcatgtgc ccctgctcac atcttcactg
     1141 gtcatcccgg taccttcgcc agcgatgttc atcgacacgc acgcgcacct ctatcacaag
     1201 cagttcgacg tggatcgcga agagatgctc gcacgggcgg tggacgcggg tgtgggccgc
     1261 ttctacctgc ctaacatcga cctggaaagt gttgcg
//
LOCUS       DAOQOC010001051         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_93530_length_1294_cov_3.699758, whole genome shotgun sequence.
ACCESSION   DAOQOC010001051 DAOQOC010000000
VERSION     DAOQOC010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93530_length_1294_cov_3.699758"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1294
                     /locus_tag="PL106_14315"
     CDS             <1..>1294
                     /locus_tag="PL106_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNA34297.1"
                     /translation="GRRGSNAQEPSAQVDLFGTTMDADGNVALTEFSTIDTVKHRYLL
                     EDTNEKMYMLAAQLKKQPRFCFDTETTGTDERTAELVGLAFSFKANEGYYVPVPADRQ
                     EAQRIVDIFKPVLENEAIGKVAQNAKYDIRVLARYGVELKGPLFDTMVAHYLLKPDQQ
                     KHGMDDMAEAYLNYRPVSITALIGEKGRGKVQKSMREVDPELVKEYAAEDADITWQLA
                     QRFGPLLEQDEVTALFNEVEMPLVHVLADMETEGIRIDIPALKQFSEELGTDLLRLQE
                     EIHAASGVPFNIDSPKQLGDVLFETLKLGGDSVKKTAKTGQYQTSEDILQGLSNAHPV
                     VPLILDYRSLRKLKGTYVDTLPEAADPVTHRVHTSYLQTVAATGRLASNDPNLQNIPI
                     RTEKGREIRKAFVPRDANYQLLSADYSQIELRIIAHMSG"
BASE COUNT          293 a          400 c          403 g          198 t
ORIGIN      
        1 cggtcggcgc gggtccaacg cgcaggaacc gtccgcgcaa gtggacctgt tcggcaccac
       61 catggatgcg gacggcaacg tggcgttgac ggagttctcc accatcgaca cggtgaagca
      121 ccgctacctg ctggaggaca ccaacgagaa gatgtacatg ctcgcggccc aactgaagaa
      181 acagccgcgt ttctgtttcg ataccgagac cacgggaacc gatgagcgta cggcggagct
      241 ggtgggattg gcgttcagct tcaaggcgaa cgagggctac tacgtgcccg tgccggccga
      301 ccgccaagag gcccaacgca tcgtggacat cttcaaaccc gtgctggaga acgaggccat
      361 cggcaaggtg gcgcagaacg ccaagtacga catccgcgtg ctggcgcggt acggcgtgga
      421 gttgaaggga cccctcttcg ataccatggt ggcgcactac ctgctgaagc ccgaccagca
      481 gaagcacggc atggacgaca tggccgaggc ttacctgaac taccgccctg tgagcatcac
      541 cgcactcatc ggcgagaagg ggcgcggcaa ggtgcagaag tccatgcgcg aggtggaccc
      601 ggaactggtg aaggagtacg ccgccgagga tgccgacatc acctggcagc tcgcccaacg
      661 attcgggccg ctgttggagc aggatgaagt gaccgcgctc ttcaacgaag tggagatgcc
      721 gctggtgcat gtgctcgccg acatggagac cgaaggcatc cgcatcgaca tccccgcgct
      781 gaaacagttc agcgaagagt tgggcacgga cctcctccgc ctccaggagg agatccatgc
      841 ggccagcggt gtgcccttca acatcgacag ccccaagcaa ctgggcgacg tgctctttga
      901 aacgctgaaa ctcggtggcg acagtgtgaa gaagaccgcg aagacgggcc agtaccagac
      961 cagtgaggac atcctccagg gtttgagcaa cgcgcacccg gtggtaccgc tcatactgga
     1021 ctatcgcagt ctgcgcaaat tgaaggggac ctatgtggat acgcttccgg aagccgctga
     1081 ccctgtcacg catcgggtgc atacaagcta cctgcagacc gtggcggcca ccgggcgcct
     1141 ggccagcaac gacccgaacc tccagaacat ccccatccgc accgagaaag gccgcgagat
     1201 ccgcaaggcc ttcgttccgc gcgatgcgaa ctaccaactg ctcagtgccg actactcgca
     1261 gatcgaattg cggatcatcg cgcacatgag tggc
//
LOCUS       DAOQOC010001052         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_93737_length_1293_cov_2.836834, whole genome shotgun sequence.
ACCESSION   DAOQOC010001052 DAOQOC010000000
VERSION     DAOQOC010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93737_length_1293_cov_2.836834"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PL106_14320"
     CDS             <1..858
                     /locus_tag="PL106_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA34298.1"
                     /translation="LIVYTEGRTSPREDTYATGEGRFEQGRLVVLVDEGSASASEIVT
                     GAMQDWDRALVVGRRSFGKGLVQRPVMLPDGSAVRLTVSRYYTPSGRCIQKSYADGKE
                     AYQQEKRDRLKHGELTSADSIHTADTVRYYTVNKRVVYGGGGIMPDVFVPIDTSLSSD
                     YFGQLVRKGVLNTYALTYVDAHRDEIQRAHRNAGSFTRSFVITRPIMDGLVAYAEREG
                     VEYEPKGLEKARQLIELRLKALIARDMWDTSAYWQTINGDNPLDRSFQRALEVLADDT
                     FQRLGLAQH"
     gene            979..>1293
                     /locus_tag="PL106_14325"
     CDS             979..>1293
                     /locus_tag="PL106_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34299.1"
                     /translation="MKDTIKIALLAIIAGTLIIQTLGGSKEGSSGTPAVAANQMASAT
                     QVAAPPTKTTFDPTVPPSPANAPVPTGPSTSVQFPVMAHDFGKVKQNSENTYVFKFTN
                     TGK"
BASE COUNT          276 a          424 c          387 g          206 t
ORIGIN      
        1 ctcatcgtgt acaccgaagg gcgtacctcg ccccgggagg acacgtatgc caccggcgaa
       61 gggcgttttg aacaaggacg cctggtggtg ctggtggacg agggcagtgc cagtgccagt
      121 gagatcgtca ccggagccat gcaggactgg gaccgcgcac tggtggtggg ccgccggtcc
      181 ttcggcaaag ggctggtgca gcgcccggtg atgttgcccg atggcagtgc cgttcgcctc
      241 accgtgagcc ggtactacac ccccagcggt cgctgcatcc agaaatccta cgccgatggc
      301 aaggaagcgt accagcagga gaagcgcgac cgcctcaaac acggcgaact cacctcggcc
      361 gacagcatcc ataccgccga taccgtacgc tattacacgg tgaacaaacg ggtggtctac
      421 ggtggtggag gcatcatgcc cgacgtgttc gtacccattg atacctcgct cagttcggac
      481 tatttcggcc agctcgttcg caagggcgtg ctcaacacct acgcgctcac gtacgtggat
      541 gcgcaccgcg acgagatcca gcgcgcccac cggaacgcgg gttcgttcac ccgctccttc
      601 gtcattaccc gaccgatcat ggacgggctg gtggcgtacg cggagaggga aggcgttgag
      661 tacgaaccga aggggctgga aaaagcccgc caattgatcg aactgcggtt gaaagcgttg
      721 atcgcgcggg atatgtggga cacctcggcc tactggcaga ccatcaacgg cgacaatccc
      781 ctggaccgca gtttccagcg ggcgctcgag gtgcttgccg atgacacctt ccagcgactt
      841 ggcctggcgc aacactgagg tcttgttgat cgggtgttaa tggtccgggc gcagcgcctg
      901 cccaccacct attttcgcgg cccatccacc aagcgcgccc cctgaaacgg gggaagaacg
      961 acgaacacga acgaacacat gaaagacacc atcaagatcg ccctgctggc catcatcgcc
     1021 ggcacgctca tcatccagac cctcggcggt agcaaggaag ggtcctcggg aacaccagcg
     1081 gtagccgcga accagatggc ctccgcaacc caggtggcgg caccgccaac caagaccacc
     1141 ttcgacccta ccgtgccccc cagcccggcc aatgcgcccg tacccaccgg gcccagcacc
     1201 agcgtgcagt tccccgtgat ggcgcacgat ttcggcaagg tgaagcagaa ctctgagaac
     1261 acctacgtct tcaagttcac caacacgggc aag
//
LOCUS       DAOQOC010001053         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94173_length_1290_cov_2.676923, whole genome shotgun sequence.
ACCESSION   DAOQOC010001053 DAOQOC010000000
VERSION     DAOQOC010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94173_length_1290_cov_2.676923"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PL106_14330"
     CDS             <1..729
                     /locus_tag="PL106_14330"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA34300.1"
                     /translation="VPYIGPSMETIRIDTPQNVVIEHHVATIVGRGLASMLDWTVLAG
                     WTILVYQIIDGLDISFGSFPQWFWILIFGIPWTFYNLLFELFMDGKSIGKRALDLKVA
                     RLDGGQPGLGHYLMRWMLRPIDALAFVGAVVILFNGKGQRLGDIAAGTCVVSMKRRYQ
                     LSDTLLADIPEGYVPVHPEVLRMGDAHARLIQRVLRSTSQRRAKTIQAMTDRVKSELG
                     ITTELHAEELLRSVLMDYVYLTGR"
     gene            complement(1082..>1290)
                     /locus_tag="PL106_14335"
     CDS             complement(1082..>1290)
                     /locus_tag="PL106_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34301.1"
                     /translation="FTGIYFMPRNFTLFLDPMDGVPVEALRAAASEWVAANKVRLALV
                     LITWFVLLKAYAVVAIARHREVGQ"
BASE COUNT          240 a          387 c          409 g          254 t
ORIGIN      
        1 gtcccgtaca ttggccccag catggaaacg atccggatcg acacaccgca gaacgtggtg
       61 atcgagcatc acgtggccac catcgtgggc cgcggactgg cctccatgct ggattggacc
      121 gtgctggccg gatggacgat cctggtgtac cagatcatcg atgggctgga catctccttc
      181 ggatcctttc cgcagtggtt ctggatcctg atcttcggga tcccgtggac cttctacaac
      241 ctgctgttcg agctgttcat ggatggcaag agcatcggca agcgcgcgct ggacctgaag
      301 gtggcgcggc tcgatggcgg ccaacccgga ctgggccact acctcatgcg ctggatgctc
      361 cggcccatcg atgcgttggc cttcgttggg gcggtggtga tcctgttcaa cggcaagggg
      421 cagcgcttgg gcgatatcgc tgcgggcacc tgcgtggtct cgatgaagcg tcggtatcaa
      481 ttgagcgaca cattgttggc cgacatcccc gagggctacg tcccggtcca tccggaggtg
      541 ctccgcatgg gcgatgcgca cgcacgcctg atccagcggg tcctgcggag caccagccaa
      601 cgcagggcga agacgatcca ggcgatgacc gatcgggtga agagcgagct cgggatcacc
      661 actgaactgc acgccgagga gctacttcgc agcgtgttga tggactacgt gtacctcact
      721 gggaggtagg ggagggcgcg gctcctgcaa ccggggccgg tgccgcactg atccttgatg
      781 tatggtatgc ggttatctgc catatcctca ttcttggtcc ggcggatgaa cggatcaacc
      841 tgtcagacga tgaggaccat cagcacctgt caagtgcatc tgccagctta tccatgctgc
      901 cgcaccaccc gccgatgtgg ctttcgcgcg ggtcttcgct gtccggacca ggttgcctga
      961 attccagtcg agtgcgcttg tcattcttgt acaggtccac ggtcacctgg ggccggtgat
     1021 cgaggttgca aaatgcacga gcttggagcc ttgcgcgaga tgcgggcctt cacccaccaa
     1081 gctattgccc cacctcccgg tgccgggcaa tggcgacaac ggcgtacgct ttcaacagca
     1141 cgaaccaggt gatcaacacc agcgcgaggc gcaccttgtt cgcagccacc cattctgagg
     1201 ctgcagcacg caatgcctcc acgggcacgc catccatggg gtccaggaag agggtgaagt
     1261 tgcgcggcat gaagtagatg cccgtgaagg
//
LOCUS       DAOQOC010001054         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94424_length_1288_cov_4.984590, whole genome shotgun sequence.
ACCESSION   DAOQOC010001054 DAOQOC010000000
VERSION     DAOQOC010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94424_length_1288_cov_4.984590"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1288
                     /locus_tag="PL106_14340"
     CDS             <1..>1288
                     /locus_tag="PL106_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34302.1"
                     /translation="FKPIKVEGHLMMDGGLYNNFPSDVMYEDFMPDYIIGSNVAFNGP
                     PPSEDDLMSQLRAIMVEKTDYSVPCDPGLIILPRTATTLFDFTTSRQAVKDGYDAAME
                     RMLEIKANIVRRVPPDEVRARRAAFRARLPHAVFGRIIVEGLKPNQTRYCERILDTER
                     TPVSAEDLKSRYFRLYADQNVSDLFPRSTWVPERGDHDLNILVKREKRVEARFGGLIS
                     TRPVNTGMLGIRYKFFGHTSAFLDGAGWFGKFYTGGQLKLRMDLRTAAPFYLEPSITI
                     HRWDWFRGFNDFFRETRPAYIVSRELWGGMSAGMGMGNKGLLRVDARYARTRDEYYQT
                     QEFGNRDTADVTDFNFGSGGLVLERNSLNRKQHPNAGELLRVDIRYVSGDEHTIPGST
                     TADRAPSHKRHDWFTAQVTLDKYFFSRKKVRFGIMA"
BASE COUNT          272 a          393 c          410 g          213 t
ORIGIN      
        1 acttcaagcc gatcaaggtg gaagggcacc tgatgatgga cgggggcctg tacaacaact
       61 tcccgagcga tgtgatgtac gaggacttca tgcccgacta catcatcggg agcaatgtgg
      121 ccttcaacgg accaccgccc agcgaggacg acctgatgag ccagttgcgc gcgatcatgg
      181 tggaaaagac ggactacagc gttccctgcg accccggcct gatcatcctg ccccgcacgg
      241 ccaccaccct gttcgatttc accacctcga ggcaggcggt gaaggacggg tacgacgccg
      301 ccatggaacg catgctggag atcaaggcga acatcgtgag gcgcgtaccg cccgacgagg
      361 tacgtgcgcg aagggccgcg ttcagggcga ggctgcccca tgcagtgttc ggccgcatca
      421 tcgtggaggg gctgaaaccg aaccagaccc gttattgcga acgcatcctg gacaccgagc
      481 gcacccctgt gagcgcggaa gatctgaagt cgcgctactt ccgcctgtac gcggaccaga
      541 acgtgagcga cctcttccca cgttcgacat gggtgccgga gcgcggtgat cacgacctga
      601 acatcctggt caagcgcgag aaacgggtgg aggcgcgttt cggcgggctc atcagcacgc
      661 gcccagtgaa cacgggcatg cttggcatca ggtacaaatt tttcgggcac acgagcgcct
      721 ttctcgatgg ggccggatgg ttcggcaagt tctatacggg agggcagctc aagctgcgca
      781 tggaccttcg cacggccgcc ccgttctacc tggagccgtc catcacgatc caccggtggg
      841 actggttccg cggattcaac gacttcttcc gggagacgcg gccggcctac atcgtgagcc
      901 gggagttgtg gggcggaatg agcgcgggca tgggcatggg caacaagggg ctgctccggg
      961 tggatgcccg gtacgcacgc acgcgcgacg agtactacca gacacaggaa ttcggtaacc
     1021 gggacacggc ggacgtcacg gacttcaatt tcggcagtgg cggtctggtg ctcgaacgca
     1081 acagcctgaa ccgcaaacag catcccaacg ccggcgaact gttacgggtg gacatccgct
     1141 acgtgagcgg cgacgagcat acgataccgg gatccacgac cgcggaccgc gcgccctcgc
     1201 acaaacgcca cgactggttc accgcccagg tcacattgga caagtatttt ttcagtcgca
     1261 agaaggtgcg cttcggcatc atggctga
//
LOCUS       DAOQOC010001055         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94443_length_1288_cov_3.816707, whole genome shotgun sequence.
ACCESSION   DAOQOC010001055 DAOQOC010000000
VERSION     DAOQOC010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94443_length_1288_cov_3.816707"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PL106_14345"
     CDS             <1..313
                     /locus_tag="PL106_14345"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNA34303.1"
                     /translation="STASGLSEWFCDDVDIHGDLFTFRWKGEGQTAQCLGRKHGEMIR
                     FQWVEDDDEGSWFELRIRIDQMTNETCLIVTDHAWPGDIETSKALWDSQVHTLQRVLG
                     A"
     gene            497..>1288
                     /locus_tag="PL106_14350"
     CDS             497..>1288
                     /locus_tag="PL106_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNA34304.1"
                     /translation="MKKLHLMMVRAYLGPLLITFIIVLFILNMQFLWKYVDDLMGKGL
                     PWITVAQLLFYAAASAVPLAIPLAVLLSSIMTMGGLGERSELVPMRSAGLGLFRIMLP
                     LTMTALLIAALSFWFSNNALPLAELKFRSLLWDVTHKKPALNLRPGVFFNGIDGFTIR
                     ISGKDEESGELRDVLIYDHRSAFQSDRTVVRAERGRMKRSTDGNYLILELENGHVYDE
                     QAAIGGQRGDRPLLRGRFARDELRLDLSGLALKRTDEDLFKDNYRM"
BASE COUNT          249 a          389 c          406 g          244 t
ORIGIN      
        1 gagcacggcc agcggcctga gcgaatggtt ctgcgacgat gtggacatcc acggggacct
       61 gttcaccttc cgttggaagg gcgaaggcca gacggcgcaa tgtcttggac gcaagcacgg
      121 cgagatgatc cgtttccagt gggtggagga tgatgacgag ggctcctggt tcgaattgcg
      181 gatccgcata gaccagatga ccaacgagac ctgcctgatc gtgacggacc acgcttggcc
      241 aggcgacatc gagacgagca aagcgctttg ggactcgcag gtacacacgt tgcaacgtgt
      301 gctgggggca tagggtcgta cgatcgccgg gccgagcagg gtacgagtgc gcattgtgcc
      361 ttcatgatca tcagaagtcc ggcacaccct ccttgcgagg tgtgtcgttc aaggagatcg
      421 ctgcacatgc cgcacaaaag gtccggtctt tgaggcgcgg tagtttcgcg gccgcgcagc
      481 cgttcccggg atcaccatga agaaattgca cctgatgatg gttcgggctt acctgggtcc
      541 gctcctcatc acgttcatca tcgtcctgtt catcctgaac atgcagttcc tgtggaagta
      601 cgtggacgac ctgatgggca aaggacttcc ctggatcacg gtggcccaat tgctgttcta
      661 cgcggcggca agcgccgtac cgctggccat cccgttggcc gtgctgctct cgtccatcat
      721 gaccatgggt ggcctgggcg agcgcagcga actggtgccc atgcgcagcg cgggcctcgg
      781 gctcttccgg atcatgctgc ccctcacgat gaccgccctg ctgatcgcgg ccctctcctt
      841 ctggttcagc aacaacgcgc tgccattggc cgagttgaag ttccgcagcc tgctatggga
      901 cgtcacccac aagaaaccgg ccctcaacct gcgccccggc gtcttcttca atgggatcga
      961 cggcttcacg atccggatct cgggcaagga cgaggagagc ggcgaactgc gcgacgtgct
     1021 gatctacgac catcgcagcg ccttccagag cgaccgcacg gtggttcgcg ccgagcgcgg
     1081 ccgcatgaaa cgcagcaccg acggcaacta cctgatactg gaactggaga atgggcacgt
     1141 atacgacgag caggcggcga tcggaggcca gaggggcgat cgtcccttgc tgcgtggccg
     1201 cttcgcgcgc gatgagctcc gccttgacct gagcggattg gcgctcaaac gcaccgacga
     1261 ggatcttttc aaggacaatt accgcatg
//
LOCUS       DAOQOC010001056         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94505_length_1288_cov_2.592863, whole genome shotgun sequence.
ACCESSION   DAOQOC010001056 DAOQOC010000000
VERSION     DAOQOC010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94505_length_1288_cov_2.592863"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PL106_14355"
     CDS             <1..1162
                     /locus_tag="PL106_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34305.1"
                     /translation="TTGHCTRLTMNNTSTYLPQSGGDINPAPRGALALLGDPALRMMN
                     LAMPANLVVTNNGGNASFNWTAASGSPAGYHIFRFGSDGLPVQVNTTLITGTSFNSTQ
                     PFVSGAEYMVRAAKLETTASGSWWNLSLGAQATAPTGANVLANVAMLLEGPYDPFSGM
                     MNDQLRVAGLIPLTEPYTALGLSQAAGGGGETTTPAVLNVTGSNAVVDWVRVELRSSG
                     SPGTIVATRQGLVQRDGDVTAVDGISALSFNAAPGNYHVAVRHRNHLGAMTASPVALS
                     GTATPLNFKIPGLGTWGTNAQKLIGGARVLWAGDATSNGQVKYTGAGNDRDPILTIVG
                     SNSPNGIVNGYSTRDVNLNGQVKYTGSGNDRDPILLNVGSASPNATRIGQLP"
BASE COUNT          260 a          418 c          416 g          194 t
ORIGIN      
        1 gaccacaggc cactgtaccc ggctcacgat gaacaacacg agcacgtatc tcccgcagag
       61 cggcggcgat atcaacccgg ccccacgcgg agccttggcc ctgcttggcg atccggcact
      121 gcggatgatg aacctggcca tgcccgcgaa cctggtggtc acgaacaacg gggggaacgc
      181 atccttcaac tggaccgccg ccagcggctc cccggcgggg taccacattt tcaggttcgg
      241 gtccgacgga ctgcccgtgc aagtgaacac caccctgatc acgggaacga gcttcaacag
      301 cacgcaacca ttcgtgtccg gggcggagta catggtgcgc gcggcgaaac tggagaccac
      361 cgcgagcgga tcgtggtgga acctctcact cggtgcgcaa gccacggcac ccaccggcgc
      421 caacgtgctg gcgaatgtgg ccatgctgct ggagggcccc tatgacccct tcagtgggat
      481 gatgaacgac cagctacgcg tagcggggct gatcccattg accgaaccat acacggcact
      541 gggcctctcc caggccgcag gcggcggcgg agaaacgacg acacccgccg tgttgaacgt
      601 aacgggttcc aacgcggtgg tggattgggt gcgcgtggaa ctgcgatcaa gcggatcgcc
      661 cggtaccatc gttgcgacca ggcaggggct ggtgcagcgc gacggcgacg taacggccgt
      721 ggacggcatt tccgcactgt ccttcaatgc ggctcccggc aactatcacg tggccgtgcg
      781 acatcgcaat cacctcgggg cgatgaccgc gagccccgtg gccttgagcg gcacggccac
      841 ccccttgaac ttcaagatcc cgggcctggg cacctgggga acgaacgccc agaagctcat
      901 cggcggcgcg cgggtcctgt gggcgggcga tgccacatcg aacgggcagg tgaaatacac
      961 gggcgccggt aatgaccgcg atccgatcct gacgatcgtg ggcagcaatt cccccaatgg
     1021 cattgtgaac ggctacagca cccgcgacgt caacctgaac ggccaggtga aatacacggg
     1081 cagtgggaac gaccgcgacc ccatcctgct gaacgtgggg agtgcctcgc cgaacgctac
     1141 gcgcatcggg cagcttccct gaacggggaa ccggaggatc cggtgcgtgc ggcacggttc
     1201 ccggcatcct tcgaataatt attccaatta gtcggcagat gaacgcggat cacctggggt
     1261 gccccgaccg ctgagaacgg atgctgcg
//
LOCUS       DAOQOC010001057         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94752_length_1286_cov_4.088546, whole genome shotgun sequence.
ACCESSION   DAOQOC010001057 DAOQOC010000000
VERSION     DAOQOC010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94752_length_1286_cov_4.088546"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1286
                     /locus_tag="PL106_14360"
     CDS             <1..>1286
                     /locus_tag="PL106_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNA34306.1"
                     /translation="VALGGDVNLTWAVPPDPDNSFQFYVVYGSPVQAGPYTALDSIFV
                     YGQSTWTHPGAGADTGPRYYYLTTVFTDTPLSESAPGDTLGTLFLQVTQSTPLGSAVL
                     DWTLLHEPPLSTTGTGQMIQMEHPLGTWTQIGLVDALTDGYQQVITICSDTLTYRIAV
                     LDQLGCVSYSNLDGAMFADVTAPAPPIMTAVSVDTATGQSVIDWAPSPDTDTEGYLVI
                     PWYNGVPNAFLAATVAGQNNTVYTWPGSSPGSQPEQYSVAAFDSCLTGDPPEPNTSAS
                     NQPHTTIHTTTSYDRCAATCTVSWSPYIGWPVDHYEVYAGFNGGTPALIATVPDPTLT
                     YQHSGLVPFATYRYVVKAVGDSTSMRSLSNQVAQVTDYPALPQSNYLRTVTVTGPDHI
                     TIVDSVDQAASVRKYRLERTNNGGPWEEVATQPGSLS"
BASE COUNT          238 a          415 c          386 g          247 t
ORIGIN      
        1 gtcgccctgg gaggtgatgt gaacctcacg tgggccgtac cacccgatcc ggacaactcc
       61 ttccagttct atgtggtgta cggaagtccc gtgcaagccg ggccatacac ggctctcgat
      121 tccattttcg tgtatggcca gtccacctgg acgcatcccg gtgccggtgc cgataccgga
      181 cctcgttatt attacctgac cacggtgttc accgatacgc cgttgagtga atccgctccc
      241 ggcgatacgc tcggtaccct tttcctgcag gttacccaga gcactccgct gggcagcgcc
      301 gtgctcgact ggaccttgct gcacgagccg ccgttgtcca ccaccgggac cggccagatg
      361 atccagatgg aacacccgct gggcacgtgg acgcagatcg gcctggtgga tgcactgacc
      421 gatggctacc agcaggtgat caccatttgt tcggacacgc tcacgtaccg gatagccgta
      481 ctggatcagt tgggttgcgt gagttacagc aacttggatg gtgccatgtt cgccgatgtg
      541 accgcaccgg cgccacccat catgacggcc gtgagtgtgg ataccgccac cggccagagc
      601 gtgatcgatt gggcgccgag cccggatacg gacacggagg gctacctggt gatcccttgg
      661 tacaatgggg tgcccaatgc attcctggcc gccacggtcg ccggacagaa taataccgtg
      721 tacacatggc cgggcagttc gcccgggtcg cagccggagc agtattccgt tgccgcgttc
      781 gattcctgcc tcaccggtga tccgcctgag cccaatacga gtgcttcgaa ccagccgcac
      841 accaccatcc acaccaccac cagctacgat cgctgcgccg ccacgtgtac cgtgagttgg
      901 tcgccgtaca tcggctggcc ggtggatcac tacgaggtgt atgcgggttt caatggggga
      961 accccggccc tgatcgccac ggtgccggat ccgacgctga cctaccagca ttccggactg
     1021 gtcccgttcg ccacctatcg ctatgtggtg aaggcggtgg gcgattcgac ctccatgcgc
     1081 tccctgagca accaggtggc gcaggtgacg gactaccccg cgctgccgca aagcaattac
     1141 ctgcgcaccg tgacggtgac cgggccggac cacatcacca tcgtcgacag cgtggaccag
     1201 gcggccagtg tgcgtaaata ccgtttggaa cgtacgaata atggtgggcc gtgggaagag
     1261 gtggccacgc aacccggttc cctgtc
//
LOCUS       DAOQOC010001058         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94757_length_1286_cov_3.731925, whole genome shotgun sequence.
ACCESSION   DAOQOC010001058 DAOQOC010000000
VERSION     DAOQOC010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94757_length_1286_cov_3.731925"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1286
                     /locus_tag="PL106_14365"
     CDS             <1..>1286
                     /locus_tag="PL106_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34307.1"
                     /translation="SSYLWSTLATSPSITISTACTYWVTVSQNGCSISDSIEVTVGPV
                     PLVDLGNDTTLCAQATLMLSAVVPGSTYHWTTGNVTSSIMINVAGTYGVTVNLGGCTA
                     YDEVTVDYVPTLTLELGPDVTLCPEDELQLNASFPGGTTVWSTNTVGSSITVDDPGIY
                     WATITVSSCSVSDSIIIDEVPLPSLDLGPDNTVCEGDAVAFDITVPGASYLWDDGSTG
                     PVRSVSVAGIYWANVMLGGCLTTDTFDLSVSPIPMVDIGPDTTVCQGALLLLDATSSG
                     AAYLWQDGSTGATFNVTGSGSYTVQVSVGTCTVEDAVNVIVNPVPIVDIGPDLSLCEG
                     EETVLDATIPGATYVWQDGSTGPSYTVTAAGTVSVDVELNGCVGTDAATISYIPLPVV
                     DLGPDTGICSGSVLQLNAMQPAGSYLWNDGNTSATVS"
BASE COUNT          235 a          403 c          390 g          258 t
ORIGIN      
        1 agcagttacc tctggagcac gctggcgacc tcccccagca tcacgatctc gacagcgtgc
       61 acctactggg tgaccgtttc gcagaacggc tgttccatct cggactccat cgaggtgacg
      121 gtagggcctg tacccctggt ggatctcggg aacgacacca ccctctgcgc acaagccacc
      181 ctgatgctga gcgcagttgt acctggatcg acctatcatt ggaccacggg caatgtcacg
      241 tcatcgatca tgatcaacgt ggccgggacg tacggggtta ccgtgaactt gggaggttgc
      301 acggcctacg acgaggttac cgtcgactac gtccccaccc tgacgcttga actggggccc
      361 gatgtcacct tgtgccccga ggacgagctg caactgaacg cgagtttccc gggaggcact
      421 acggtgtgga gcaccaacac ggtcggttcg tccatcaccg ttgatgatcc gggcatctac
      481 tgggccacca tcacggtgag ttcctgttcc gtcagcgact cgatcatcat cgacgaggta
      541 cccttgccat cgctcgatct gggcccggac aacacggtat gtgaaggtga cgcggtggcc
      601 ttcgatatca ccgtacccgg agcgagttac ctctgggacg atggcagcac cggtcccgtg
      661 cgttcggtga gcgttgccgg catctattgg gccaacgtga tgctcggtgg ctgcctcacg
      721 accgacacgt tcgacctgtc cgtttcgccc atcccgatgg tggatatcgg tcccgacacc
      781 acggtatgcc agggcgcgct gttgctcttg gacgccacaa gttccggggc tgcgtacctc
      841 tggcaggatg gcagcaccgg cgccaccttc aacgtgaccg gttcgggcag ttacaccgtg
      901 caggtctcgg tgggcacgtg taccgtggag gatgcggtga acgtgatcgt gaaccccgta
      961 ccgatcgtgg atatcggccc ggacctgtcg ctctgtgaag gtgaagaaac cgtgctggat
     1021 gccaccatcc ccggtgccac ctatgtctgg caggacggaa gtaccggtcc ctcgtacacg
     1081 gtgacggccg cgggcacggt cagtgtcgac gtggaactga acggctgtgt cggcaccgac
     1141 gctgccacca tttcctacat cccgttgcct gtggtggacc tgggccccga taccggcatc
     1201 tgttccggca gcgtgcttca gttgaacgcc atgcaacccg ctggcagcta cctgtggaac
     1261 gacgggaata ccagtgcgac agtatc
//
LOCUS       DAOQOC010001059         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_94943_length_1285_cov_2.947154, whole genome shotgun sequence.
ACCESSION   DAOQOC010001059 DAOQOC010000000
VERSION     DAOQOC010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94943_length_1285_cov_2.947154"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /gene="gatC"
                     /locus_tag="PL106_14370"
     CDS             <1..269
                     /gene="gatC"
                     /locus_tag="PL106_14370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNA34308.1"
                     /translation="IAELARLDVSDPAERERLLTDMQRVLDFVEKLNEVDTTGVEPLI
                     HMSEEHDVLREDVALPGLSKQETLMNAPVKDSDYFKVPKVVDKG"
     gene            469..>1285
                     /locus_tag="PL106_14375"
     CDS             469..>1285
                     /locus_tag="PL106_14375"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNA34309.1"
                     /translation="MSITTTLRGMMTRVALLASLFGPGNMLSAQELRTVPMPGCGFDE
                     GHQQRMRLDPLYRQRVEAFNAAARHAAPGEDRGALTYTVPVVVHVMDIGTAATAISDE
                     QIRAGIERLNENWRKVAGTQGDGTGVDMGIQFALAVRDPQGNCTSGITRRDMTFSATY
                     VANGVMHNGAVGMPDAELKSYDNWDPTRYYNIWLVGEIDNGGPVAGYAYFASSHGSSI
                     DGTILLAGYMPGSNVLSHELGHAFNLYHTFEGDANGTTCPPNGDCTSDGDHVCD"
BASE COUNT          261 a          379 c          424 g          221 t
ORIGIN      
        1 gtatcgccga actggcccgt ttggacgtga gcgaccctgc tgaacgcgag cgactgttga
       61 cggacatgca gcgcgttctc gatttcgttg agaagctcaa tgaggtggat accacgggcg
      121 tggaaccatt gatccacatg agcgaagagc acgatgtgct gcgcgaggat gtcgcactcc
      181 ccggcctgag caagcaggag accttgatga acgcgcccgt gaaggacagc gactacttca
      241 aggtgcccaa ggtggtggac aagggctgag ccctcgatcg ctgttttcgg gagggcggac
      301 cggatcgcgc accggacggg tgggtgaaca ccctttttat gaacagtcgg gcaaacaggt
      361 gtttctactg gtatcggtgt aacggcggac cacctacatt gcgtgtcctt ttgtaccctg
      421 aacgaaggga ccttcccgaa ccacgaacct gttaccccat cccttacgat gagtatcacc
      481 acgaccctgc gcggcatgat gacgcgcgtt gcgctgctcg cctccctgtt cggccccggc
      541 aacatgctct cggcccagga actccggacg gtgcccatgc ccggctgcgg attcgatgaa
      601 ggccaccagc aacggatgcg actggatccg ctgtaccggc agcgtgtgga ggcgttcaac
      661 gcggccgccc ggcatgccgc acccggcgag gacaggggtg cgctcacgta cacggtgccg
      721 gtggtggtgc acgtgatgga catcggcacg gcggccacgg cgatcagcga cgagcagatc
      781 agggcgggga tcgaacgcct caacgagaac tggcgcaagg tcgcgggaac acaaggcgac
      841 ggcacgggtg tggacatggg tatccaattc gcgctggccg tgcgcgaccc gcaggggaat
      901 tgcacatcgg gcatcacccg gcgcgacatg acgttcagcg cgacgtatgt ggccaacggc
      961 gtgatgcaca atggtgcggt gggcatgccg gacgcggaac tgaagtccta cgacaactgg
     1021 gatccgacgc ggtactacaa catctggctg gtgggcgaga tcgacaacgg tggaccggtg
     1081 gcgggctatg cgtacttcgc cagcagccat ggatcctcga tcgacggtac gatcctgctt
     1141 gccggctaca tgcccggaag caatgtgttg tcgcacgaat tgggacacgc gttcaacctc
     1201 taccacacct tcgaggggga cgcgaacggg acgacctgtc cgccgaacgg ggactgcacc
     1261 tccgatggcg atcatgtgtg cgaca
//
LOCUS       DAOQOC010001060         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_95082_length_1284_cov_3.286412, whole genome shotgun sequence.
ACCESSION   DAOQOC010001060 DAOQOC010000000
VERSION     DAOQOC010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95082_length_1284_cov_3.286412"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PL106_14380"
     CDS             complement(<1..813)
                     /locus_tag="PL106_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34310.1"
                     /translation="MDDADRLTPERSPNESVRMLSLFRQNRPLVLLMLPLLLALLWPG
                     ASPVEAPLTHGPPGMPAHQAFRLLLASANWTLPVLACMLVAGLAVQLNFTVNSSGLFE
                     ERNHVPALLLPLLLALYPQGLVPDPALSGMPFILWALRRLWSSVGQQRILSPLADAGL
                     LIGLAACFHLPYAFMVVVIWSTLAITRPLQWREYLVPLLGLVVILFLLWGLMHLFFPG
                     VWDPLGSVRPAQRPAGPQSPVPHWLRGVVLWALAGILLVSAAISFAAGYARGI"
     gene            846..1028
                     /locus_tag="PL106_14385"
     CDS             846..1028
                     /locus_tag="PL106_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34311.1"
                     /translation="MQDFFFAIGHFCQKTFDWFLVSLGWITPVVFAIVLAIGAAYWMM
                     SQARYTRKAIERNERI"
BASE COUNT          252 a          432 c          403 g          197 t
ORIGIN      
        1 gataccacgt gcgtagcctg ccgcgaagga gatggccgca ctcaccaaca ggatcccggc
       61 aagggcccac agtacgaccc cgcgcaacca gtggggcacg ggcgactgcg gaccggcggg
      121 tcgttgggcg ggccgcacgg accccagtgg gtcccatacg ccggggaaaa agagatgcat
      181 cagtccccaa agcaggaaga ggatcacgac caggcccagc aggggaacca ggtattcacg
      241 ccactgcagc ggccgggtga tcgccagggt ggaccagatg accaccacca tgaacgcgta
      301 gggcaggtgg aagcaggcgg ccaggccgat gagcaggccc gcatcggcga gcggactgag
      361 gatccgctgc tggcccaccg aagaccacag ccgacgcagc gcccagagaa tgaagggcat
      421 gcccgacaag gccgggtcgg gaaccagccc ttgcgggtac aaggccagga gcaacggcag
      481 caacaacgcg ggcacatggt tccgttcctc gaagagcccg ctgctgttca ccgtgaagtt
      541 caattgcacg gcgagtccgg ccaccaacat gcaggcgagc acaggcaggg tccagttcgc
      601 actggccaac agcagccgga atgcctgatg cgccggcatg ccgggggggc cgtgcgtgag
      661 cggcgcttcc accggtgatg cccccggcca cagcaacgcg agcaggaggg gcagcatcag
      721 cagcaccagc gggcgattct ggcggaacag gctcagcatg cgcacacttt cgttcgggga
      781 ccgctccggg gtgagccggt ccgcatcgtc catctatatt cgcccccgct caagtacaca
      841 gccacatgca ggacttcttt ttcgccatcg gccatttctg ccagaagacc ttcgattggt
      901 tcctcgtttc gctgggctgg atcaccccgg tggtttttgc cattgtcctg gccattggcg
      961 cagcgtactg gatgatgtcg caggcgcggt acacgcgcaa ggccatcgag cgcaacgagc
     1021 gcatctgatc ccggtccgtc attcctcacc agcattcggg agcggcttcc gttgagcccg
     1081 gctcctgcgt ccaggccacg ccaccaacac gctctcacgc gctcgtcgtc acgttcttcg
     1141 gggcctgact tttcaccagg tccttcccga tgtcggtgcg tacggtgcgg ccctcgaagt
     1201 cgatcatcac cacgttccgg tacgtattgg ccaccgcctg ctccacatcc ttgccgaagc
     1261 ccgacacggc gagcacccgt ccac
//
LOCUS       DAOQOC010001061         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_95083_length_1284_cov_3.272579, whole genome shotgun sequence.
ACCESSION   DAOQOC010001061 DAOQOC010000000
VERSION     DAOQOC010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95083_length_1284_cov_3.272579"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="coaE"
                     /locus_tag="PL106_14390"
     CDS             <1..621
                     /gene="coaE"
                     /locus_tag="PL106_14390"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNA34312.1"
                     /translation="GPLARERMRPMFKVGITGGIGSGKSTVARVFGVLGIPVFDADSE
                     SKRLLVEDADVKGAVIAAFGATIYPGGRLDRAALAAAVFNNPEALGRLNAIAHPAVRK
                     RLAHWTDEQRSPYVLVESALLVDTGWYRSMDHLIVVSAPEAERVRRVMARDLTTEDQV
                     RARMRNQVGEEERLAVADSIIRNDGTTLVIPQVLALHDALLARAFL"
     gene            706..984
                     /locus_tag="PL106_14395"
     CDS             706..984
                     /locus_tag="PL106_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34313.1"
                     /translation="MTNSRHLPLSSVTLVFGVLSIPLAFVRHLCVPATIMALLAVLFS
                     MWGRWMVEKGRTFDDRGLRRSALGLKLALVGGVCGVLMWVLWATGALL"
     gene            1042..>1284
                     /locus_tag="PL106_14400"
     CDS             1042..>1284
                     /locus_tag="PL106_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNA34314.1"
                     /translation="MQALTLVEGRAFALTLQRLCHQLIEDHGDLSDTALIGLQPRGVH
                     FSRRLKQELERDLPGTVITYGELDITFHRDDFRHRSV"
BASE COUNT          212 a          414 c          408 g          250 t
ORIGIN      
        1 ggcccattag ctcgtgaacg catgcggccc atgttcaagg tcgggatcac cggaggcatc
       61 ggcagtggca aaagcaccgt ggcgcgcgtg ttcggcgtgc tgggcatccc tgtctttgat
      121 gccgatagcg agagcaaacg cctgctggtc gaggacgccg acgtgaaggg ggcggtgatc
      181 gcggcattcg gtgcgaccat atatccgggt gggcggttgg atcgggcggc cctggccgca
      241 gccgtgttca acaaccccga agcactcggc cggttgaatg ccatcgcgca tcccgccgtg
      301 cgcaagcgcc tggcacattg gaccgatgaa cagcgctcgc cgtacgtcct ggtggaatcc
      361 gcactgctgg tggataccgg ctggtaccgc agcatggacc acctcatcgt tgtatcagca
      421 cccgaggccg aacgggtgcg ccgtgtgatg gcccgtgatc tcaccaccga agaccaggtg
      481 cgcgcccgca tgcgcaacca ggtgggggag gaggaacgcc tcgcggtggc cgacagcatc
      541 atccgcaacg atggcaccac gctggtgatc ccacaggtgc tcgcgttgca cgatgcgctg
      601 ctggcccggg ctttcctgtg agatcatgga gcagggaacc ggtccgcggg ttttcaggcc
      661 cagttccccc ggcctcaccc accttcgcac ccgtgccttt tgatcgtgac gaactcccgc
      721 caccttccac tttcctcggt cacgctcgtt ttcggggtgt tgagtatccc gctggcgttc
      781 gtgcggcacc tgtgcgtgcc cgccaccatc atggccctgc tggccgtcct cttcagcatg
      841 tggggcaggt ggatggtgga aaagggccgc accttcgacg acaggggtct gcggcgttcc
      901 gcgctcggac tgaagcttgc cctggtgggc ggtgtatgcg gcgtgctcat gtgggtgctt
      961 tgggccacgg gcgcactgct ctgaccctcg caagtacgcc atcggtcgtg gatcaaggga
     1021 tccaaggtat tttcgccacc gatgcaggcg ctcacccttg ttgaaggccg ggccttcgcg
     1081 ctgaccctcc agcgtctctg tcatcaattg atcgaagatc acggggacct ttcggatacc
     1141 gcccttatcg ggctccaacc gcgcggcgtc catttcagcc gcaggctgaa gcaggaactc
     1201 gaacgcgacc ttccaggaac ggtcatcacc tacggtgaac tggacatcac cttccatcgc
     1261 gacgacttcc ggcatcgctc cgtg
//
LOCUS       DAOQOC010001062         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_95219_length_1283_cov_3.694625, whole genome shotgun sequence.
ACCESSION   DAOQOC010001062 DAOQOC010000000
VERSION     DAOQOC010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95219_length_1283_cov_3.694625"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PL106_14405"
     CDS             <1..294
                     /locus_tag="PL106_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34315.1"
                     /translation="GGIYVYGGLTDFQCQKDFRMTWTPEEKAYTLRAKVKQGFVNYCY
                     AYLPPDSDVPDLTTIEGSHFQTENDYLVLVYVKDYALHCDRLVGVRFVNSRRG"
     gene            complement(325..>1283)
                     /locus_tag="PL106_14410"
     CDS             complement(325..>1283)
                     /locus_tag="PL106_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34316.1"
                     /translation="YDPGGDRYFGSCTDDNSIRQRAQDGTVSAFVVDAGGAPYGIELM
                     GDTLFANVGGSVRGFRTSDGSMVSDLDLGASFLNGLTTDGHFLYTTDFSNKTILKVDP
                     VAGTFSTLVSNTVQTPNGIVFDPALNRLWVACWGGNARIKSYDRESGAELSSYTTSLG
                     NIDGITLDCNGDILVASWSPGRITRFEPSFTAPAGTVVGTGLSSPADIDYDDVHHVVC
                     VPNSGNNTVVLPEVTDCSTSVGGNGTYRTINAYPNPADGRVRVDLPVSGPEPFLLMGP
                     TGLLVGSGTLLPGGLLDVSTLPVGTYVFDFTGLMRYVRVVKK"
BASE COUNT          269 a          392 c          389 g          233 t
ORIGIN      
        1 ggcggcatct acgtgtacgg tggactgacg gacttccaat gccagaagga cttccgcatg
       61 acttggacac ccgaggagaa agcatacact ttacgggcca aggtgaagca aggcttcgtg
      121 aactactgct acgcgtacct cccaccggat tccgacgtac ccgatctgac caccatcgag
      181 ggctcgcact tccagacgga gaacgactac ctggtactgg tgtacgtgaa ggattatgcg
      241 cttcactgcg accggttggt gggcgtgcgc ttcgtgaaca gcagaagagg ttgaccaacc
      301 gtgtgaaaag gagcagggaa ctcctcactt cttcaccacc cgcacgtacc gcatcagtcc
      361 ggtaaaatcg aaaacatacg taccaactgg gagtgtgctc acatcgagca gtcccccggg
      421 aagcagggtt ccgctgccca ccagcaagcc ggtgggcccc atcagcagga agggttcagg
      481 gccggatacg ggcaggtcca cacgcacacg accgtcggcg ggattcgggt aggcgttgat
      541 cgtccggtag gttccattgc cacccaccga tgtggagcaa tccgtcacct cgggcagcac
      601 cacggtattg ttgcccgaat tgggcacgca caccacatga tgaacatcgt cataatcgat
      661 gtcggcgggg ctgcttaggc ccgtgcccac cacggtcccg gccggtgcgg tgaaggaagg
      721 ctcaaagcgc gtgatacggc cgggcgacca cgaggccacg aggatatcgc cgttgcaatc
      781 gagggtgatc ccatcgatgt tgcccaggct ggtggtatac gagctcaact ccgcaccgct
      841 ctcccggtcg tagctcttga tgcgcgcatt gccgccccag caggccaccc acaggcggtt
      901 gagtgcgggg tcgaatacga tgccgttggg ggtctgcacc gtattgctca ccagcgtgct
      961 gaaggtgcct gccactggat ccaccttgag gatggtcttg ttgctgaaat cggtcgtata
     1021 aaggaaatgc ccatcggtgg tgagcccgtt gaggaaggag gccccgaggt ccagatcgga
     1081 taccatgctg ccatcgctcg tgcggaagcc acgcaccgag ccgcccacgt tggcgaaaag
     1141 cgtgtcgccc atgagttcga tgccgtacgg tgcaccgccc gcatccacca cgaaagccga
     1201 caccgtgccg tcctgcgccc gctggcggat gctgttgtca tcggtgcagc tcccgaaata
     1261 gcgatcaccc ccgggatcgt act
//
LOCUS       DAOQOC010001063         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_95263_length_1283_cov_2.942182, whole genome shotgun sequence.
ACCESSION   DAOQOC010001063 DAOQOC010000000
VERSION     DAOQOC010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95263_length_1283_cov_2.942182"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..827)
                     /locus_tag="PL106_14415"
     CDS             complement(195..827)
                     /locus_tag="PL106_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34317.1"
                     /translation="MNKLLCALAGLCCLTASAQRDPQRPLVQFSGVVVTGDSLLPVPF
                     AQIATVGSYRGTVSDVFGYFSFVAQVGDTLGFAAVGFKRDWFVVPDTVKESKYSMIHV
                     MHPDTILLDPVMVTPWPSKEQFAQAFLNLRLTEDAYQTALRHLNSAEAIQRMENFPSD
                     PGLSQQMGTAMANTKLYQQGGMPTISLLNPIAWAQFMQAWRSGKFKSKKQ"
     gene            903..>1283
                     /locus_tag="PL106_14420"
     CDS             903..>1283
                     /locus_tag="PL106_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNA34318.1"
                     /translation="MERAARAAKLVLYLVALCVAFAVDMQDKVLNGSRPRLLAAGTAL
                     PSRYTAIVPGCRVNGDDPSACLEERLQKALELYRNGHVQRLLLSGDHGATGYDEVNAM
                     RTWLLDHGVAADHVFLDHAGFDTWD"
BASE COUNT          220 a          380 c          425 g          258 t
ORIGIN      
        1 ggcaacgcct gtgccaaagt tattcggagc ctgcgatccg tggcgtggaa tgcgtgcaga
       61 gccgtggggt tcgatgacct ttcaactgca ccggggactg gagcaaggca ccggtcgcgg
      121 ggggcgtacg gcccttggtc gtgttccaag ctcatcggtc acgctcgatc ccgggccggg
      181 ccgccaccag gccgctactg ctttttgctc ttgaacttcc cgcttcgcca cgcctgcatg
      241 aactgcgccc aggcgatcgg gttcaacagg ctgatcgtcg gcatacctcc ttgttgatat
      301 aatttcgtat tcgccatggc cgtgcccatc tgctggctca atccggggtc gctcgggaag
      361 ttctccatgc gctggatggc ctcggcgctg ttcaggtgcc gcaacgccgt ctggtacgcg
      421 tcctcggtga gccggaggtt caggaaggcc tgtgcgaact gttccttgct gggccacggg
      481 gtcaccatca cgggatccag caggatggtg tccggatgca tcacgtggat catggagtat
      541 ttcgattctt tcaccgtatc gggcaccacg aaccagtcgc gcttgaaacc cacggcggcg
      601 aagcccagcg tgtcgcccac ctgggccacg aagctgaagt acccgaatac gtcgctcacc
      661 gttccccggt agctgcctac cgtggcgatc tgcgcgaagg gcaccggcag caggctgtcg
      721 cccgtgacca ctacgccgct gaattgcacc aagggccgtt gtgggtcccg ttgcgctgag
      781 gcggtaaggc agcagagacc ggccagggcg cagaggagct tgttcatcgg gacgaaggta
      841 gggccgcacc tgcacagagc atgccatcgg cagagccggg tatgcgtgta gtttggcccc
      901 cgatggaacg tgcggccagg gcagcgaagc tcgtgttgta cctggtggcc ctgtgtgtgg
      961 cctttgccgt ggatatgcag gacaaggtgc tgaatggatc gcgaccgcga ctgttggcag
     1021 cgggcactgc gctgccaagt cggtacaccg ccatcgtgcc cggttgtcgg gtgaacggtg
     1081 atgatccgtc ggcctgcctg gaagaacgac tgcaaaaggc gctcgaactt tatcggaacg
     1141 gccatgtgca acggctgctc ctgagtggcg atcacggcgc caccggatat gatgaggtga
     1201 acgccatgcg cacctggttg ctggatcatg gcgtggccgc cgatcatgtg ttcctggacc
     1261 acgcgggttt tgacacgtgg gat
//
LOCUS       DAOQOC010001064         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_95390_length_1282_cov_3.074980, whole genome shotgun sequence.
ACCESSION   DAOQOC010001064 DAOQOC010000000
VERSION     DAOQOC010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95390_length_1282_cov_3.074980"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PL106_14425"
     CDS             <1..527
                     /locus_tag="PL106_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA34319.1"
                     /translation="FDLEAEHIINVTDEESALLEQAFKDIIAEHARFSHEKDHVLRNL
                     IHILLLRIREIHRPHAHHIKESASRASELTDRFKHLVEKNFIEHRQVAWYADKLHITP
                     KHLADVVKQTIARTPRQVIADMLMLEAKVLLGSTDHTISQIAQQLHFDDQAHFAHFIK
                     QRSGQSPTELRTKL"
     gene            730..1206
                     /locus_tag="PL106_14430"
     CDS             730..1206
                     /locus_tag="PL106_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNA34320.1"
                     /translation="MNRNLISGLLFLGAGSAIALTCAFTNPKTSNSMIDTMTKSEAKS
                     FEKPDEVRTFPKGRLELINIGGATVGRGIFEPGWRWSTSVMPIAKTKSCEAPHFQYHV
                     AGTLHVVMDDGTEYDLKPGDVSILPSGHDAWTVGNEAVVVVDFQGMVDYASGVHKH"
BASE COUNT          292 a          422 c          332 g          236 t
ORIGIN      
        1 tcttcgatct ggaagccgaa cacatcatca atgtcacgga tgaggagagc gcactgctcg
       61 aacaggcgtt caaggacatc atcgccgagc atgcgcgctt cagccacgag aaggaccatg
      121 tgctgcgcaa cctcatccac atcctgctgc tccggatccg cgagatccac cggccgcacg
      181 cacatcacat caaggaaagc gcatcgcgcg ccagcgagct caccgatcgt ttcaagcatc
      241 tggtcgagaa gaacttcatc gagcatcgcc aagtggcctg gtatgcggac aaacttcata
      301 tcacgcccaa acatctggcc gatgtggtga agcagaccat cgctcgtaca ccccgacagg
      361 tgatcgcgga catgctcatg ctcgaggcga aggtgctgct cggctccacg gaccatacca
      421 tttcgcagat cgctcagcaa ctccatttcg acgaccaggc ccacttcgcg catttcatca
      481 agcagcgatc aggccagtcg cccactgagc tccgtacgaa gctctaacgc acaatatcag
      541 cacaccccct ccccggtggt aatcctcgcc attcggatcc cgacatccgg atcccgacat
      601 ccggatcctg gacctggata gcgcggacac ggatcaccct cttgttcgca tccgtacaag
      661 ttctaccgtg ttccatccaa ggatgtggcc ttggcgtgcc gacacttttg ccccatcaaa
      721 ccacggcaca tgaatagaaa cctgatctcc ggcctcctgt tcctcggtgc aggatcggct
      781 attgccctca cctgcgcgtt cacaaacccc aaaacctcca attccatgat cgacacgatg
      841 accaagagcg aagccaagag cttcgagaag cccgatgagg tgcgcacctt ccccaaaggc
      901 cgcctcgaac tgatcaacat cggcggtgcc accgtgggcc gcggcatctt cgagcccggc
      961 tggcgctgga gcacgagcgt gatgcccatc gccaagacga agagctgcga ggcgccacac
     1021 ttccagtacc acgtggccgg taccttgcac gtggtgatgg acgatggcac cgaatacgac
     1081 ctgaaacccg gtgatgtgtc catcctcccc agtggccacg atgcctggac cgttggcaac
     1141 gaagcggtgg tggtcgtgga tttccagggc atggtggatt acgcttccgg ggtccacaag
     1201 cattgagcgc cgacacccgc aacagcgaat gcccgggcat tcgctgtatg aacagggggt
     1261 atcacaattt gtgatacccc ct
//
LOCUS       DAOQOC010001065         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_95690_length_1280_cov_3.896327, whole genome shotgun sequence.
ACCESSION   DAOQOC010001065 DAOQOC010000000
VERSION     DAOQOC010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95690_length_1280_cov_3.896327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PL106_14435"
     CDS             complement(<1..1112)
                     /locus_tag="PL106_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34321.1"
                     /translation="MNASWTRLWQGMDPRFGGCEEGAECRVSARKFVWGDPPLTPFGS
                     ARANFALRTYLLDKSPYLSTEALQEMVARNSMPQAMQVEVMIANPDATRRDGFIQWVE
                     LNAPVPFPTYMLAQVEASWDERTYRTTLEEQMGEHHANMTQAANALLQYYAKDTVGEP
                     IDSLRWVWQQVRTPAARYAEALTYMAQGNYPSANEVVEDIPEEHDLKAPEMVERQRML
                     DLIAFLQGVQLDGRTEAELDSVEVDALEALVADAHDRPAVWAQNLLCYAYDRCRSWPT
                     GGDGMDWRALSAGQNEQPTFEPLGLRINPNPAETWVALNYNLPKAAPTGELRLLDVTG
                     RLLWSRKLADTYGQVVVDTRTWSTGSYTVELRQGERT"
BASE COUNT          227 a          423 c          366 g          264 t
ORIGIN      
        1 gtacgctctc cttggcgcaa ctccaccgta tagctaccgg tgctccatgt acgcgtgtcc
       61 accactactt gcccgtaggt gtcggccagc ttcctgctcc acaacaagcg gccggtaacg
      121 tccaacaacc gcagttcgcc ggtcggcgct gctttcggca aattgtagtt caacgcaacc
      181 caggtctccg ccgggttggg gttgatgcgc aaccccaaag gttcgaacgt cggctgttca
      241 ttttgccccg cgctcaatgc gcgccaatcc atgccgtcgc ctccggtggg ccaagaccgg
      301 cagcggtcgt acgcatagca cagcaggttc tgtgcccaca cggcaggccg gtcgtgcgca
      361 tcggcaacaa gtgcttccag ggcatccacc tccacgctat ccaattcggc ttcggtacgg
      421 ccatccaact gcacgccctg caaaaaggcg atcaggtcca gcatgcgctg gcgttccacc
      481 atttccgggg ccttcaggtc gtgctcctcg gggatgtcct ccaccacctc gttcgccgag
      541 gggtaattcc cctgggccat gtacgtgagc gcctcggcgt agcgtgcggc cggcgtgcgc
      601 acctgctgcc acacccagcg caaactgtcg atgggttcgc ccaccgtatc cttggcgtag
      661 tactgcagca atgcgttggc ggcctgggtc atgttggcgt ggtgctcgcc catctgttcc
      721 tccaaggtgg tgcgataggt gcgctcgtcc caactcgctt ccacctgggc cagcatgtac
      781 gtggggaagg gtacgggcgc gttcaattcc acccattgta tgaagccatc cctccgggtg
      841 gcatcggggt tggcgatcat cacctccacc tgcatggcct ggggcatgga gttgcgggcc
      901 accatctcct gcagggcctc ggtgctcagg tacgggctct tgtccagcag gtaggtgcgc
      961 agggcaaagt tcgctctagc gctcccgaaa ggagtgagag gcggatctcc ccatacgaat
     1021 ttgcgagcgc taacgcgaca ttcagcgcct tcctcacacc ctccgaaacg gggatccatc
     1081 ccctgccaca gccttgtcca tgatgcattc atgtggtgtt ggctttccct tgggacggcc
     1141 aagggccaac aacgctaccg aacattttca accagcgcaa atcgatctga ggaacggtat
     1201 gtcaggatcc agcaacgcac aaacacgaag ggcgcggtcc tgctggccgc gcccttcgcg
     1261 tattccgtgt cctgatcagt
//
LOCUS       DAOQOC010001066         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96015_length_1278_cov_4.309894, whole genome shotgun sequence.
ACCESSION   DAOQOC010001066 DAOQOC010000000
VERSION     DAOQOC010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96015_length_1278_cov_4.309894"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PL106_14440"
     CDS             complement(<1..246)
                     /locus_tag="PL106_14440"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNA34322.1"
                     /translation="MSIDRHDSLLALTRMRTAIAQVRKHVEGCTMEQFMANKRTSDAV
                     LMQLSVLGEEVRRVDPDLLAKYPYPWHEVRAQRNVIAH"
     gene            complement(236..838)
                     /locus_tag="PL106_14445"
     CDS             complement(236..838)
                     /locus_tag="PL106_14445"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA34323.1"
                     /translation="MATAQPQPFPALLRSHRETAGKTLREVAEKLKVDVSLISKWERG
                     ERKPTRTQVNALAKYLKTDSKTLLTAWLRDAVVYAVGDDELALDAIKAAEAQVVYQHF
                     AKQDRNTIVRKLKAGLARFPKVRRAWLFGSFARKDDKPGSDIDLAIEVQDPFSYFDLA
                     DVQYQLEQLVGRKVDVGFMDTFRPHVRARIEPDLRLIHEH"
     gene            complement(884..>1278)
                     /locus_tag="PL106_14450"
     CDS             complement(884..>1278)
                     /locus_tag="PL106_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNA34324.1"
                     /translation="VVSRGGSLLLNVAPGPDGQLDSTAYVRLKEVGAWLKVNGEAVYG
                     TSALAVDPNRSGVIGATTGKDGSTYLFCEPGKQVAWPWGGGARPKATGLANGERLRPI
                     LREGIIYIAAPASANNRGVSVIKLEPQP"
BASE COUNT          216 a          410 c          389 g          263 t
ORIGIN      
        1 gtgggcgatc acattgcgtt gggcacgcac ctcatgccac ggatacgggt acttggccag
       61 caggtcgggg tccacccggc ggacctcctc gccaagcacg ctcagttgca tgagtaccgc
      121 gtcgctggtg cgtttgttgg ccatgaattg ttccatggta caaccttcca cgtgcttgcg
      181 tacttgagca atggccgtgc gcatccgcgt caatgccagg aggctgtcgt ggcggtcaat
      241 gctcatggat cagtcgcagg tcaggctcga tgcgtgcgcg cacatggggg cggaaggtgt
      301 ccatgaagcc cacgtccact ttgcggccca ccaattgctc cagttggtat tgcacatcgg
      361 ccaggtcgaa atagctgaag gggtcctgca cctcgatggc cagatcgatg tcgctgccgg
      421 gtttgtcgtc cttgcgggca aaggagccga agagccatgc cctgcgcacc ttggggaagc
      481 gcgccaggcc cgctttcagc ttgcgcacga tggtgttgcg gtcctgcttg gcgaagtgct
      541 ggtacaccac ctgcgcttcc gccgccttga tcgcatccag cgccagttca tcatcgccca
      601 cggcgtacac cacggcatcg cgcagccatg cggtaagtaa ggtcttgcta tcggttttca
      661 ggtatttggc caaagcgttg acctgcgtgc gggtcggctt gcgctcaccg cgctcccatt
      721 tgctgatcag gctcacatcc accttgagct tttcagccac ttcgcgcaag gtcttgcctg
      781 cggtttcacg atgcgaacgc aacagtgccg ggaagggttg gggctgggct gtcgccatgt
      841 tggacagata ttgtccaaag ataagggcaa ggggcgaagg gcgctacggc tgcggctcga
      901 gcttgatcac gctcacgccc cggttgttgg cagaggccgg tgccgcgatg tatatgatcc
      961 cttcgcgcag gatcgggcgg agccgttcgc cattggccaa cccggtggcc tttggccgtg
     1021 cgccccctcc ccacggccac gccacttgct tgccgggttc gcagaacaaa taggtggaac
     1081 catccttgcc ggtggtagcg ccgatcaccc cggagcggtt ggggtccacc gccaacgcac
     1141 tcgtcccgta caccgcctcc ccgttcacct tcagccaagc ccccacttcc ttcaagcgca
     1201 catacgccgt ggagtcgagt tgcccatccg gccccggcgc cacgttgagc aggagacttc
     1261 caccgcgcga cacgactt
//
LOCUS       DAOQOC010001067         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96058_length_1278_cov_3.234669, whole genome shotgun sequence.
ACCESSION   DAOQOC010001067 DAOQOC010000000
VERSION     DAOQOC010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96058_length_1278_cov_3.234669"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PL106_14455"
     CDS             <1..969
                     /locus_tag="PL106_14455"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA34325.1"
                     /translation="TDARFASYNNLPQGTYTFQVRASHNGYDWGPPAGFEFHVAPPFW
                     LRWWFFVCCAAIIGLVLFTVMRYRALRRERHEKTRQLMLRSRMLQLEQQALNANMNRH
                     FIFNALNSIQFYINRQDRPAANRYLTSFAKLIRKNLDASRTDTTTLAEELERLDLYLV
                     LEHMRFKDRFVYELRVDPAVDTSRIELPAMMLQPYVENSIWHGILPMERQGLVLIHVE
                     PGLPGHVRITIQDDGIGVESSRDRKATEPSDHISRGIEITKGRADVLRRLDLADIRIT
                     GPEQLHDPNGAPCGTRVTIELPVAVGTIRTNGVLEISQDQLTFGRP"
     gene            complement(1049..>1278)
                     /locus_tag="PL106_14460"
     CDS             complement(1049..>1278)
                     /locus_tag="PL106_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34326.1"
                     /translation="SCHAIGHSGDSRHPMAPPFRYLSQNYRVNELEEAFAEGVLVGHP
                     DMPEFTLSAEDVDALLSYIQSVQERRSAKRQ"
BASE COUNT          243 a          410 c          366 g          259 t
ORIGIN      
        1 accgatgcgc gtttcgcctc gtacaacaac ctgccgcagg gcacctacac cttccaggtg
       61 cgagcctccc ataatggcta cgattggggc ccccccgccg gttttgagtt ccatgtggcc
      121 ccgccattct ggctccgctg gtggttcttc gtttgctgtg cggccatcat aggactggtg
      181 ctgttcaccg tgatgcgcta ccgtgccttg cgccgtgagc gacacgagaa aacacgccag
      241 ttgatgctgc gctcgcgcat gttgcaattg gagcagcagg ccctcaacgc caatatgaac
      301 cgccacttca tcttcaatgc gctcaacagc atccagttct acatcaaccg gcaggaccgc
      361 cctgcggcca accgctacct caccagcttc gccaaactga tccgcaaaaa cttggacgcc
      421 agccgcacgg acaccaccac cttggccgag gaacttgagc gactggacct ttacctcgtg
      481 ctcgaacaca tgcggttcaa ggaccgcttc gtgtacgaat tgcgggtcga cccggccgtg
      541 gacaccagcc gcatcgaatt gcccgccatg atgctgcagc cttatgtgga gaacagcatc
      601 tggcacggca ttctgcccat ggagcggcag gggctggtgc tcatccatgt ggaacctggc
      661 ctgcccggtc atgtgcgcat caccatccag gatgatggga tcggggtgga aagcagccgt
      721 gaccgcaaag ccaccgaacc ctcggatcac atcagccgcg gcatcgagat caccaagggg
      781 cgggccgatg tgctgcgtcg attggacctg gccgatatcc gcatcaccgg ccccgaacag
      841 ctccatgatc cgaatggtgc gccctgcggc acccgggtga cgatcgaact gcccgtggcc
      901 gtcggaacca tccgcaccaa cggtgttttg gaaatttcgc aggatcagct tacctttggg
      961 cgtccatgaa cgcatccgct caaacgggtc tcttccggta ttcgctggtg gtcgcggtcc
     1021 ttgccgtgat cttcgcggtg cttgcgtttc actgacgttt ggcgctgcgg cgttcttgca
     1081 cgctctggat atagctcagt agcgcgtcga catcttcggc ggagagcgtg aattcaggca
     1141 tgtcgggatg gcccaccagc acgccttccg cgaacgcttc ctcaagctcg ttgacgcgat
     1201 aattctgtga caaatagcga aacggcggcg ccatcggatg gcggctgtcg ccggagtgcc
     1261 cgatggcatg gcaactgg
//
LOCUS       DAOQOC010001068         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96061_length_1278_cov_3.186427, whole genome shotgun sequence.
ACCESSION   DAOQOC010001068 DAOQOC010000000
VERSION     DAOQOC010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96061_length_1278_cov_3.186427"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..466
                     /locus_tag="PL106_14465"
     CDS             98..466
                     /locus_tag="PL106_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34327.1"
                     /translation="MGSPISYSTHGPTLAYRTFGHGPRSVLAFHGFGRTHNILLASGK
                     PPYLWGPDKPDMKGISVIHDETKKKRYVQIDLDLIGRKRGEIDDYLDLLLIEARRDEP
                     VIAFEEVEKRLKKPRIRTKR"
     gene            469..723
                     /locus_tag="PL106_14470"
     CDS             469..723
                     /locus_tag="PL106_14470"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA34328.1"
                     /translation="MYQVSFKRSAERELNRLREPLRTRVMHAIGSLRADPRPHGSIKL
                     KGSSNAYRIRVGDHRVLYTVDDMVRIVLIQQVGDRKDVYR"
     gene            749..>1278
                     /locus_tag="PL106_14475"
     CDS             749..>1278
                     /locus_tag="PL106_14475"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA34329.1"
                     /translation="MDMAHYFTHGITLAYRIYGHGPLPVLAFHGFGRTGADFAVLEHE
                     LGDRCTIHAFDLHFHGESPGYPERTNKPFTPMELAGYFTAFMDHIGAQKVLLLGYSLG
                     GRLALNLLEQVPERIQRAFLVAPDGLKTRPWYRALATSSMGRWLYGRFVKDPTVAHAI
                     IHALHRTRLMSDKMHH"
BASE COUNT          257 a          422 c          363 g          236 t
ORIGIN      
        1 ttgataacca caacgacgcc atggaggcaa cggacccgct gggttcttga tcttcgtggc
       61 gctaccgccg cgctcattgt gccgttgcgg tggaagaatg ggctcaccga tctcctattc
      121 cacccacggc cctaccctcg cctaccggac cttcggccat gggccgcgat cggtgctggc
      181 cttccatggc ttcgggcgca cgcacaacat actcctggcc tccggcaagc cgccctacct
      241 ttggggtcca gacaagccgg acatgaaagg catcagcgtc atccacgacg aaacgaagaa
      301 gaaaaggtac gtgcagatcg acctggacct gatcggcagg aaacgtggcg agatcgatga
      361 ctatctcgat ctgctgctga tcgaagcccg cagggatgag cctgtcatcg cgttcgagga
      421 agtggagaaa cggctgaaga agccacgtat ccgcaccaag cgttgacgtt gtatcaggtc
      481 tccttcaaac gttccgccga acgggaactg aacaggctcc gcgaaccgct ccgtacccgg
      541 gtcatgcatg ccatcggttc cctgcgcgcc gacccgcgcc cgcacgggtc catcaagctg
      601 aaaggctcct cgaacgcata ccggatccgc gtgggcgatc accgggtgct gtataccgtc
      661 gatgacatgg tgcgcatcgt actgatccaa caggtcgggg accgaaaaga cgtgtatcgg
      721 tagcggcgtt ccggccatac gtaccctgat ggatatggcc cattacttca cccacggcat
      781 caccttggcc taccgcatct acggccacgg accgctgccg gtgctggctt tccacggctt
      841 cggtcgcacg ggcgccgact tcgccgttct ggaacacgaa ctgggcgacc gctgcaccat
      901 tcatgccttc gacctgcact tccatggcga gagccccggc tatccggagc gcaccaacaa
      961 gcccttcacg ccgatggagc tggccgggta cttcaccgcc ttcatggacc atatcggggc
     1021 acaaaaggta ctcttgctgg gctacagctt gggcgggcgc ctcgcattga accttttgga
     1081 gcaggtgccg gaacgcattc aacgagcctt cctggtcgcg cccgacggtc tgaaaacccg
     1141 gccctggtac cgcgcactgg ccacctccag catgggacgc tggctgtatg gtcgcttcgt
     1201 gaaagatccc acggtggcgc acgccatcat ccatgcactg caccgcaccc ggctgatgag
     1261 cgacaagatg caccactt
//
LOCUS       DAOQOC010001069         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96106_length_1278_cov_2.409648, whole genome shotgun sequence.
ACCESSION   DAOQOC010001069 DAOQOC010000000
VERSION     DAOQOC010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96106_length_1278_cov_2.409648"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PL106_14480"
     CDS             <1..1121
                     /locus_tag="PL106_14480"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA34330.1"
                     /translation="DYDLLLRLTERIVQGDKRRSIGVGRQSDRIARVPRVLYHWRIHA
                     GSAARGEDVKPYAYDAAKRALTEALARRGEPAEVSFLYGFRGYDIRFTSPLKGKVSVV
                     IPTKDKADILATCLRSLFNLTDHPDFEVIVVSNNSTERALFDLMREMEAAHGERFRWF
                     EHNVPFNFSGLMNEGTRQATGDHILFLNNDTEIIHADWMRSMHAWSQRPSIGAVGVKL
                     LYHNDTIQHGGVVIGLGGVAGHVFTGTHKDGPGYFNYVNTINNYSAVTAACMMVERKK
                     LEAIGGWEELFTVEYNDVDLCLRLREKGWNNVYLPHVSLYHYESLTRGHPHMTSASYE
                     RHLREVSLFKERWKGYVEDDPCYNPALSRGRHDWHFEL"
BASE COUNT          265 a          376 c          399 g          238 t
ORIGIN      
        1 aggactacga tctgctcctg cgcttgaccg aacgcatcgt acagggggac aagaggcgtt
       61 cgatcggtgt tgggcgacag tccgatcgga tcgcgcgtgt gccgcgcgtg ctttaccact
      121 ggcgtatcca tgcgggcagc gcggcgcgtg gtgaggacgt gaaaccgtac gcgtacgacg
      181 cggcgaagcg cgcactcacg gaagcccttg cacgacgcgg ggagccggcc gaagtatctt
      241 tcctgtacgg cttccgcgga tacgatatcc ggttcacatc gccgttgaag ggcaaggtga
      301 gcgtggtgat ccccacgaag gacaaggccg acattctcgc gacctgcctg cgatcgctgt
      361 tcaacctcac cgatcatccg gacttcgagg tcatcgtggt gagcaacaac agcaccgaac
      421 gtgcgctctt cgacctgatg agggagatgg aagccgcgca cggtgagcgc ttccgctggt
      481 tcgagcacaa tgtcccgttc aatttcagcg gcctgatgaa cgagggcaca cgacaggcca
      541 cgggtgatca catcctcttc ctgaacaacg acacggagat catccacgcc gattggatgc
      601 gctctatgca tgcgtggagc caacgtcctt ccattggtgc ggtgggcgta aagcttcttt
      661 accacaacga caccatccag cacggcggtg tggtcatcgg actgggcggc gtggcgggac
      721 atgtcttcac cggcacgcac aaggacggcc ccggttattt caactacgtg aacaccatca
      781 acaactacag cgcggtcaca gccgcttgca tgatggtgga acggaagaag ctcgaggcga
      841 tcggtggttg ggaggagctc ttcacggtgg aatacaacga tgtggaccta tgcctccgct
      901 tgcgggagaa gggatggaac aacgtttatc tcccgcacgt gtcattgtac cattatgaga
      961 gtctcaccag aggacacccg cacatgacga gtgcgagcta cgaacgtcac ctgcgtgagg
     1021 tcagcctctt caaagagcgc tggaagggat acgtggagga cgatccgtgc tacaatccgg
     1081 ccctgagccg ggggcggcac gactggcatt tcgagctctg acgatcacag gggatcggtc
     1141 agcttgtcct ccagcaggtc atccgccgca ccgctgtgcg gctgccaggc cagcgcgctg
     1201 cgcaggtttg gcagttcatc gtcaagccgc tggagccaat cgacctgatt ggccccctgc
     1261 aaatgctcgg cggcgcgc
//
LOCUS       DAOQOC010001070         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96118_length_1278_cov_2.224857, whole genome shotgun sequence.
ACCESSION   DAOQOC010001070 DAOQOC010000000
VERSION     DAOQOC010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96118_length_1278_cov_2.224857"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PL106_14485"
     CDS             complement(<1..632)
                     /locus_tag="PL106_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34331.1"
                     /translation="MRTTRPERRPVRLVPQRTSIVLAIILSGASLSAQTTFTMSNGSG
                     QACQGVLFDSGGQGASGYQNGEDYTFTLCPDVPGNVIYLTFSNFALDESGPQNSWDNL
                     SIYDGDNTSATYMGSYTGNDLQNLIVSGTVFNVTGCLTLVFQSNSTGTGVWAAGFSCT
                     VPCQNPLAVASMSEPVPALVCQGESVSFDGSASYARPGYNIVQYLWDFDDG"
     gene            complement(847..1218)
                     /locus_tag="PL106_14490"
     CDS             complement(847..1218)
                     /locus_tag="PL106_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34332.1"
                     /translation="MSTIRPFALITAVALLTSCAKNELVAPGLPTASTSSATQGNGPH
                     LSTDELTPDLGASDANPGNGPQTGLLIDGDVIPDLGNDDSSSGNNNPTASGTIVVDLG
                     NSDTPNGTSHSGGTPHSPDTE"
BASE COUNT          230 a          344 c          446 g          258 t
ORIGIN      
        1 ccatcatcga agtcccagag gtattgcacg atgttgtacc ccggccgggc gtaggaagcg
       61 ctgccgtcaa agctcaccga ttcgccttgg cagacgaggg ctggaacggg ctcgctcatc
      121 gaggccacgg ccagtggatt ctggcagggg acggtgcagg agaacccggc cgcccataca
      181 ccggtgcccg tgctgttgga ctggaacacc aaggtgaggc agcccgtgac attgaacacc
      241 gtcccgctca cgattaggtt ctgcaggtcg ttccccgtgt aggaacccat gtacgtggca
      301 ctggtgttgt ccccgtcgta aatggacagg ttgtcccagg agttttgcgg tccgctttcg
      361 tccaatgcga aattcgagaa ggtcaggtag atcacgttac ccggcacatc ggggcagagg
      421 gtaaaggtgt agtcctcgcc gttctggtaa ccgctcgccc cttgtccgcc cgaatcgaaa
      481 agcaccccct ggcatgcctg gccactgcca ttggacattg tgaacgtggt ctgagcggaa
      541 agcgacgcac cggacaggat gatcgcgagg acgatgctcg tgcgttgcgg tacaagccgg
      601 actggtcggc gttcaggtcg ggttgtcctc atatcgtcca gatagaaggg tggggaggtt
      661 cagcgaagat agggggcgtc cccttgtgga cgcgaggatg acgcatggac cggggcgcag
      721 gttcgctgga aggaaaaaga cttgtgaacc gcggtgggat cccgaggaaa tgaacgcccc
      781 ccgtcgtggg acggagggcg tgctgggcaa gcaggaagaa ctggaagtcc cggccttgaa
      841 gcggatctac tccgtatccg ggctgtgcgg ggtaccgccg ctgtgggagg tgccgttcgg
      901 ggtgtcgctg ttgcccaggt ccaccacgat ggtcccgctc gcggtcgggt tgttgttgcc
      961 cgaggatgag tcgtcatttc ccaggtccgg gatcacgtcg ccgtcgatca gtaggccggt
     1021 ttgcggcccg ttgcccgggt tggcatcgct cgcgcccagg tccggggtca attcatcggt
     1081 gctcagatgg gggccgttgc cctgcgtggc actggaggtg gaagccgttg gcaggccggg
     1141 tgccaccagt tcgttcttgg cgcaggaagt aaggagcgca acggcggtga tcagggcgaa
     1201 aggtcggatg gtgctcatgg tatcaggtat tttttttgat cgggttcgtg atgttgacgc
     1261 tgcgaaccaa gcctgtcg
//
LOCUS       DAOQOC010001071         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96429_length_1276_cov_2.221130, whole genome shotgun sequence.
ACCESSION   DAOQOC010001071 DAOQOC010000000
VERSION     DAOQOC010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96429_length_1276_cov_2.221130"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PL106_14495"
     CDS             complement(<1..480)
                     /locus_tag="PL106_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA34333.1"
                     /translation="MELAKRYDPSTTESKWYQYWLDNGYFRSVPDGREPYTVVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMQGKNACWVPGTDHASIATEAKVVKLLAEQGIKK
                     SAIGREEFLKHAYAWKDKYGGVILEQLKKLGASCDWDRTRFTMEPKLNEAVLDVFIDL
                     "
     gene            complement(620..>1276)
                     /locus_tag="PL106_14500"
     CDS             complement(620..>1276)
                     /locus_tag="PL106_14500"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNA34334.1"
                     /translation="KADGRKTWLKDLAKKQVVFLSDKLKEDKLPDWINKYVSHKKRRI
                     GPVECKLLADHLGSDLGKLTNEVEKLCIVTEENGTITSALIERNVGISKDHNIFELQN
                     AIGVRDHLKAQTIAHHLANDRDHKLFLTVGLLSSYFSKIAIVHTKRGLSTGDLAAALK
                     VPPFFLKDYTTAARNYPVDQVRRIQHLLREADLNSKGVGNTSAEDGELLRELLFRMMN
                     "
BASE COUNT          195 a          385 c          396 g          300 t
ORIGIN      
        1 caggtcaatg aacacgtcga ggacggcctc gttcagcttg ggttccatgg tgaagcgcgt
       61 acgatcccaa tcgcagcttg cgcccagctt cttcagctgc tccaggatca ccccgccgta
      121 cttgtccttc caggcgtagg catgcttgag gaactcctcg cgtccgatcg cgctcttctt
      181 gatgccctgt tcggccagca gcttcaccac cttcgcttcg gtagcgatac tcgcatgatc
      241 ggtacccggc acccagcagg cgttcttgcc ttgcatgcgg gcgcggcgca ccagcacatc
      301 ctggatcgtg ttgttgagca tgtgccccat gtgcagcacg ccggtcacgt tgggcggcgg
      361 gatcaccacc gtgtagggct cacggccatc gggcacgctg cggaagtagc cgttgtcgag
      421 ccagtactgg taccacttgc tttccgtggt ggaggggtcg tagcgcttgg cgagttccat
      481 tgcatccggc gaagtcttgg cgaaggcgga tggggttcca ccttccgatc gtcttcgggg
      541 ccgcgaaagt accgggatag gccacgcgga gcgcagtgtg accatgacca aggccaaggc
      601 accgaccggt cgccagcgct cagttcatca tccggaacag gagttcgcgc agcagctcgc
      661 cgtcttcggc gctggtgttg cccaccccct tgctgttgag gtcggcttcg cgcagcaggt
      721 gctggatcct ccttacctgg tccaccgggt agttgcgcgc cgcggtggtg tagtccttca
      781 ggaagaaggg aggcaccttc agcgcggcag cgaggtcgcc cgtggaaagc ccccgtttcg
      841 tgtgaacaat ggcgatcttg ctgaagtagc tgctgagcag gcccacggtg aggaagagct
      901 tgtggtccct gtcgttggcc aggtggtgcg cgatggtctg tgccttcagg tgatcgcgca
      961 caccgatggc gttctgcagt tcgaagatgt tgtggtcctt gctgatgccc acgttgcgct
     1021 cgatgagcgc ggaggtgatc gtgccgttct cttccgttac gatgcagagc ttttccacct
     1081 cgttggtgag tttgcccagg tcgctgccca agtggtcggc caggagtttg cactccaccg
     1141 gcccgatcct ccgtttcttg tggctgacgt atttgttgat ccagtccggt aacttatcct
     1201 ccttcagctt gtcgctcagg aagaccactt gttttttggc gaggtccttc agccaggttt
     1261 ttcgcccgtc cgcctt
//
LOCUS       DAOQOC010001072         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96772_length_1273_cov_4.151888, whole genome shotgun sequence.
ACCESSION   DAOQOC010001072 DAOQOC010000000
VERSION     DAOQOC010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96772_length_1273_cov_4.151888"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PL106_14505"
     CDS             complement(<1..462)
                     /locus_tag="PL106_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34335.1"
                     /translation="MRQLLTASIVLLATLATAQTKQAGARSSDQRRLEFTIKGLSKDT
                     IYLAGYYGNKLLYNDTAVADAKGKVVFAKPRGYKAGVYAVVIPGPRYFEFLVNEPVVQ
                     MSSDTLDLGGALTVQKSPENQVFLDYIRFLNDKRKEADAMGKQRDAAEDPIR"
     gene            641..1006
                     /locus_tag="PL106_14510"
     CDS             641..1006
                     /locus_tag="PL106_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34336.1"
                     /translation="MQRTLATLTFCAATLFATAQSREIKVVDTDAMRVSATKHTELVH
                     KTVTLDADQRAKVQEVYMNYERQLDGLNQRFEIGGLSKEEREAEMAPQWASLERMVDE
                     QLATILTPEQLGKWQEARR"
BASE COUNT          244 a          388 c          396 g          245 t
ORIGIN      
        1 gcggatcggg tcttcggcgg catcgcgctg cttgcccatg gcatcggcct ccttgcgctt
       61 gtcgttcagg aaacggatgt agtccaggaa tacctggttc tcggggctct tctgcacggt
      121 gagcgccccg ccgaggtcca gggtgtccga gctcatctgc accaccggtt cgttcaccag
      181 gaattcgaag tagcgcggac cggggatcac cacggcatat acgcccgcct tgtagccccg
      241 gggcttggcg aagaccacct tgcccttggc atcggccacc gccgtgtcgt tgtacaacaa
      301 cttgttccca tagtaccccg ccagatagat cgtgtccttc gataggcctt tgatggtgaa
      361 ttccaagcgc cgctggtcgc tcgatcgggc acctgcctgt ttcgtttggg ccgtggccag
      421 ggtggcgagg agtacgatac tggccgtgag gagttgtcgc atgtggatca ggtaagccgg
      481 atcggcggcc aaaagtagcc gtgggcatac cccgtgccgg aaggcattaa caggctgtta
      541 agaagctgca tgccaacagg tcacagtgcg aagcgggcaa ccaggtcatg gtccgctcgt
      601 ctccaggttg tctatctttc gcccacaagc ccaacggacg atgcaacgca ccctcgccac
      661 tctcacgttc tgcgccgcca ctttgttcgc caccgcgcag agccgcgaaa tcaaagtggt
      721 cgacacggac gccatgcgcg tcagtgccac caaacacacc gaactggtgc ataagaccgt
      781 taccttggat gccgatcaac gggccaaggt gcaagaggtc tacatgaact acgaacgcca
      841 gctggacggc ctgaaccagc gattcgagat cggtgggctc agcaaggagg agcgcgaagc
      901 ggagatggct ccgcagtggg cgagcctcga acggatggtg gatgagcaac tggccacgat
      961 cctgaccccc gagcaattgg gcaagtggca ggaagcccgc cgctaacgcg gttcattcga
     1021 ccctgaacgg acgtcccgca tccgtgccgc tatctggtgg caggatccgg gacgttcgct
     1081 ttccaatacc ccggtcaggg gatcggctca ctgcgcctgg gcggtcctga attcgctggt
     1141 ggtgtggaac tcgatcgccg ggttgttgcg ccgctccagg tccagcatgt aggccgaggg
     1201 ggccatgaac accggcagtc cgtccttgtc ctggaggatc tgcaggccct tcacccggat
     1261 gaattcctga agg
//
LOCUS       DAOQOC010001073         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96882_length_1273_cov_2.077997, whole genome shotgun sequence.
ACCESSION   DAOQOC010001073 DAOQOC010000000
VERSION     DAOQOC010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96882_length_1273_cov_2.077997"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1273
                     /locus_tag="PL106_14515"
     CDS             <1..>1273
                     /locus_tag="PL106_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNA34337.1"
                     /translation="DRGGFSVHLLCVERGREHRVTVPIDVPWTNKELHVDLMSFRDKV
                     LPGAREEWRLRITGNKNARVAAQLLAAMYDASLDHFVPNSWNMDIWPRHYARQGWNAG
                     APFGTSSGQLAWWDRGLPAGSVHAPPVLNTFGFDGPLYGSHAFGYLQDAAGGPMQKSR
                     VLATMAPSPGAMEASSDAEDGDHMDEESREPEAHDAPATEVKPGAPSTTRTDFRETAF
                     FFPDLMTDKDGAVVLRFTMPEALTRWKLMGLAHTADLKLATFTKEAISQKPLMVVPNL
                     PRFLREGDRITLTAKINAIEAPVNGTSKLELFDPFTNRSISASFQLKEDTRTFSAAPG
                     QSASVAWDIAVPAGVGLVSVRITASGNGFSDGEERPLPVLTDKLLVTESLPLWNNGKG
                     TRTFTLDKLKNNTSTSLRTQALKLEYTPSPAW"
BASE COUNT          259 a          422 c          386 g          206 t
ORIGIN      
        1 gaccgcggcg gcttcagcgt tcacctgctg tgcgtggagc gcggtcgcga gcatcgggtg
       61 accgtaccga tcgatgtgcc atggaccaac aaggaactgc acgtggacct gatgtccttc
      121 cgcgacaagg tgctgcccgg ggcccgcgag gaatggcgcc tgcgcattac cgggaacaag
      181 aacgcgcggg tggcggccca actcctcgcc gccatgtacg atgcatcact cgatcatttc
      241 gtacctaatt cctggaatat ggacatctgg ccgcgccact atgcacgcca ggggtggaac
      301 gcaggggccc ccttcggcac cagcagtggc cagctcgcct ggtgggaccg tggtctgccc
      361 gccggatccg tgcatgcccc acccgtgctg aacaccttcg gcttcgacgg gccactgtac
      421 ggatcgcatg cgttcggata cctgcaagac gccgccggtg ggccaatgca gaaaagccgg
      481 gtcctggcca cgatggcgcc ttcccccggc gctatggaag caagcagcga tgcggaggac
      541 ggggaccaca tggatgagga gtcccgcgaa cctgaggccc atgacgcgcc agcaaccgag
      601 gtcaaacctg gtgcgccgtc caccacccgg accgacttcc gcgagacagc cttcttcttc
      661 cccgatctga tgaccgacaa ggatggtgcg gtggtcctgc gcttcaccat gcccgaggcc
      721 ctcacacggt ggaagctgat ggggctggcc cacaccgcgg acctgaagct cgcgactttc
      781 accaaggagg ccatcagcca gaagccgttg atggtggtgc ccaacctgcc ccgattcctg
      841 cgcgaaggcg accgcatcac gctcaccgcg aagatcaacg cgatcgaagc gccggtcaat
      901 ggcacctcga aactggaatt gttcgatccg ttcaccaaca ggtcgatcag tgcatcattc
      961 caactgaagg aggacacccg cacgttctcc gccgcccccg ggcaaagcgc cagcgtggct
     1021 tgggacatcg ccgttccagc aggcgttggc ctggtgagcg tacggatcac cgccagcggc
     1081 aatggcttca gcgatgggga ggagagaccg ttacccgtgc tcactgataa actgctcgtc
     1141 actgagagtc tgccgctctg gaacaatggc aagggcacgc gcaccttcac actggacaag
     1201 ctgaagaaca ataccagcac atcgttgcgc acacaggcac tcaagctgga gtacacgccc
     1261 agccccgcct ggt
//
LOCUS       DAOQOC010001074         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_96960_length_1272_cov_3.151191, whole genome shotgun sequence.
ACCESSION   DAOQOC010001074 DAOQOC010000000
VERSION     DAOQOC010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96960_length_1272_cov_3.151191"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1272)
                     /locus_tag="PL106_14520"
     CDS             complement(<1..>1272)
                     /locus_tag="PL106_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34338.1"
                     /translation="SATVDVQVSTYPDPGTDATLTLCAVDAPSDLFSILGGSPDPGGV
                     WTAPDGSPHEALFDPTTDTPGTYTYTIAVPAPCSSVSATVTIAVQQPPSAGSDGSLSL
                     CISGASQLLSSGLNGAPDSGGTWTDPLGNTTDATFIPGTDAPGNYTYTVAGQDPCPST
                     SANVTITVNDEPDAGLDGAIVLCVDGGPEDLFSHLGGSPDAGGQWNAPDGTLHNGNFD
                     PAIDIEGVYSYTITAPPPCSSASSSVIVAVENGPDPGTDGSIMLCISDAPVLLFNTLG
                     GTPDGNGTWTSPIGDPFDGIFDPATDPAGSYTYTLSGTPPCQMVQSIVEVSVNAPADA
                     GTTGTITACANASSMDLFAHLGGDPDLNGVWTGPDGGPHSGSLDPQVDLNGTYTYSLT
                     SQAPCPGDASVVVVTIIAPPDPGTDADLTLCT"
BASE COUNT          250 a          370 c          414 g          238 t
ORIGIN      
        1 ggtacagagc gtcaggtcgg cgtccgtgcc cggatccggt ggagcaatga tcgttacgac
       61 cactacggaa gcatcgccgg ggcatggtgc ctggctcgtc aggctgtagg tgtaggtccc
      121 attgaggtcc acctgcggat ccaacgaacc gctgtgcggg ccaccatcgg gtccggtcca
      181 tacgccgttc aggtccggat ccccaccgag atgcgcgaat agatccattg acgaggcatt
      241 cgcgcaagcg gtgatcgtgc cagtcgtgcc cgcgtcggcc ggggcattaa cggaaacttc
      301 cacgatggat tggaccatct ggcagggcgg tgtaccggaa agagtatatg tatatgagcc
      361 cgcggggtcc gttgccggat cgaagatgcc gtcaaacgga tcgccgatcg gcgatgtcca
      421 tgtgccgttc ccatccggtg tgccacccaa ggtgttgaaa agcagcaccg gcgcatcgga
      481 gatgcacagc atgatggagc catccgttcc gggatccggc ccattctcca ccgccacgat
      541 caccgaactg ctcgcacttg aacagggtgg gggagcggtg atcgtatagg agtacacgcc
      601 ttcgatgtca atcgcagggt cgaagttgcc gttgtgcaag gtgccgtcag gtgcgttcca
      661 ttggccgccc gcgtcggggg aaccacccag atgactgaag aggtcctcgg gcccaccatc
      721 aacacagaga acgatcgctc catccaggcc tgcatccggt tcatcgttca cggtaatggt
      781 gacattggcg ctggtgctcg gacaaggatc ctggccggca acggtatagg tgtagttgcc
      841 cggtgcatcg gtgccgggaa tgaaggtggc atccgtggtg tttcccagcg gatcggtcca
      901 cgtgccaccg ctatcaggcg caccgttcaa accgctcgag agcaactggg aagcgccact
      961 gatgcacaac gaaagcgaac cgtcgctccc ggccgaaggt ggctgctgga cagcaatggt
     1021 taccgttgcg ctcacactgg agcatggtgc gggcactgcg atggtatagg tgtatgtacc
     1081 tggtgtatcc gttgtcggat cgaacagggc ctcatgcggt gaaccatcag gtgcggtcca
     1141 gacacccccc ggatccgggc tgcctcccag gatggagaag aggtccgaag gtgcgtcgac
     1201 agcgcagagc gtgagggtcg catccgtccc tggatcggga taggtgctca cctgcacgtc
     1261 cacggtcgcg ct
//
LOCUS       DAOQOC010001075         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_97098_length_1271_cov_3.577303, whole genome shotgun sequence.
ACCESSION   DAOQOC010001075 DAOQOC010000000
VERSION     DAOQOC010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97098_length_1271_cov_3.577303"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PL106_14525"
     CDS             <1..285
                     /locus_tag="PL106_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34339.1"
                     /translation="HHWGRHVSPWRLLLVWPWRGPVELGVSVLLMALLIGAAVPNHLL
                     GVGPGMAWWGAHRVLGMVWLTFVLAGFTTFAWFAFFGMFSRRAWRDHRFN"
     gene            complement(319..543)
                     /locus_tag="PL106_14530"
     CDS             complement(319..543)
                     /locus_tag="PL106_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34340.1"
                     /translation="MATNTLLGYYKDILDRISFADGPTFRKELRKAFRQLLPEERTEL
                     KRWFRTQCVCRMDQQEMKALLIAMERYDRR"
BASE COUNT          215 a          390 c          390 g          276 t
ORIGIN      
        1 catcactggg gacgccatgt ctcaccctgg cgtctgctcc tggtgtggcc gtggcgtggt
       61 ccggtcgaac tcggcgtctc cgttctcctg atggcgctcc tcatcggtgc ggccgtgccc
      121 aaccacctgt tgggcgtggg acctgggatg gcctggtggg gcgctcaccg ggtgctgggc
      181 atggtctggc tcacattcgt gctcgccggc ttcaccacct tcgcatggtt cgccttcttc
      241 ggtatgttca gccgacgggc ctggcgcgat caccggttca actgatcggg gatccccgtt
      301 ccggtgcatc ggatcggatc aacgccgatc gtaccgctcc atggcgatca acaacgcctt
      361 catttcctgc tggtccatcc ggcacacgca ttgggtccgg aaccaacgct tgagttcggt
      421 ccgttcctcg gggagaagtt gcctgaacgc tttgcgcaat tccttgcgga aggtcgggcc
      481 gtccgcgaag ctgatccggt ccaggatgtc cttgtaatat ccgagcaggg tgttggtagc
      541 catgagtgta tgaagaggtg cgtcgctttc aacgcctgtg catccggaat gttccatctt
      601 tcctgaatac atggccagtt gcacgatcca tgccgcgatc cgctcatccg ccctcgtttt
      661 tggaccaagc ttgaaccagt ttcgccgcta ctgcaagaac ttgagggagc ggtgacgtca
      721 gctgcgtacg ccgctacggc atccgataac gaacaacccc gaacccgacc acaacgtgga
      781 attccgcgct gacaccgaaa gaaacgacaa gagcgggcgt cgcctgtatc gcagctacgg
      841 ccgcgatcgc gatgaggacc atggcggccg acccgacaac gcagcggaac gtggtccgat
      901 ccgtgatcca gggcatcggg gacatctccg ttcgggacct ggccttcgtc cgggtcaagc
      961 gctccgacgg tgccatcact tacccgagtt gcgtggaggg tgccgtattg cgggaacaga
     1021 ggtcctggac ccgttgccaa gtggctttga tcgggagttt caaccgctcg ggccgcgaac
     1081 tctcgtgcct gggccgcgcc gggcgtgcgg agtatcggcc cttggaaggg tgcccgacga
     1141 gcactgttca tggcgctgtt ttataccttg cccgtcaggg ggaaaatact accgatcaca
     1201 tgaatgatca caactttcca ggtctgcgct cgggcgatgt gttccctgcg gtgggtgttc
     1261 tccaacgact g
//
LOCUS       DAOQOC010001076         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_97251_length_1270_cov_3.827984, whole genome shotgun sequence.
ACCESSION   DAOQOC010001076 DAOQOC010000000
VERSION     DAOQOC010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97251_length_1270_cov_3.827984"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1270)
                     /locus_tag="PL106_14535"
     CDS             complement(<1..>1270)
                     /locus_tag="PL106_14535"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA34341.1"
                     /translation="FPIAIVNADQNTYCEGAEALFTQVGEGIIDSCQWVFSDDPADTI
                     TTVFPLIQTAHLFPLVDVPTQFVVTCLVYNPCGVATDVDTVTIVPSNITIATNTDPLI
                     GCAPLTVTFQNNTASDTSAAWYLPLPFGMVVADTVVVTFDQPGVYQVIARSYACGFDE
                     DTITVTVLEAPVPVMNAAPDPFCSGTPITFTSTAPGTNVLGWLFGDGDSSALAQPSHL
                     FDGPGNYMVTLEVGWALNQCTGSITTPVPVSASPTGGFTFGPPICAPDTVCFDATAQS
                     TDSYTWDFGNSSTEEDPCDLFTAPGVFPVSLLLANADGCTLLLDTVLVVDTLPQAVFS
                     ALVLDPCHSPAPVTFANNSTHATGYLWDFGTLGASTATTPALSIPQPGSWPVQLTVSG
                     TPGIPACIAVVDTTLVVHPEPLAVAQVLTDP"
BASE COUNT          271 a          341 c          437 g          221 t
ORIGIN      
        1 ggatcggtga gcacttgagc caccgcgagt ggttcgggat gaaccacgag ggtcgtgtcc
       61 acaacggcga tgcaggcagg aatgcccggt gtgcctgaaa cggtgagctg cacgggccaa
      121 ctgccgggtt gcgggatgct caatgcgggc gttgttgccg tggatgcgcc aagggtcccg
      181 aaatcccaga ggtagcctgt ggcatgggtc gagttgttcg cgaaggtgac cggggcagga
      241 gaatggcagg gatccaggac caatgcggag aaaactgctt gcggcagggt atccaccacc
      301 agcaccgtat ccagcaggag cgtgcaacca tcggcgttcg cgagaagcag ggacacgggg
      361 aacacccccg gagcggtgaa caggtcgcac ggatcctctt ccgtggagct gttcccgaag
      421 tcccatgtgt aactgtcggt cgattgggcc gtcgcatcga agcagacggt gtccggcgcg
      481 cagatcggcg gtccgaaggt gaagccgccc gttggtgaag cggacaccgg tacgggagtg
      541 gtgatggagc cggtacattg attcagtgcc cagccgacct ccagggtcac catgtagttg
      601 cccggtccat cgaacaaatg cgaaggttgg gccaaggcac tggaatcccc atcgccgaaa
      661 agccaaccga gcacgttggt gcccggagcg gtactcgtga aggtgatcgg cgtaccggag
      721 cagaacggat cgggggcggc attcatcacc ggtacgggcg cctcgagtac ggtaaccgtg
      781 atggtatcct catcgaatcc gcaggcatac gagcgcgcga tcacctggta cacaccgggc
      841 tgatcgaagg tgaccaccac ggtatccgcc acgaccatgc cgaagggcag tggcagatac
      901 catgctgcgg atgtgtccga agcggtattg ttctggaagg tcacggtgag gggtgcgcaa
      961 ccgataagcg gatcggtgtt cgtggcaatg gtgatgttcg acggcacgat ggtgacggtg
     1021 tccacatccg ttgccacgcc gcacgggttg tacacgaggc aggtcaccac gaattgtgtc
     1081 ggaacatcca ccaaggggaa aagatgagcg gtctggatga gcggaaaaac cgtggtgatg
     1141 gtatccgcag ggtcgtcgct gaacacccac tggcagctgt cgatgatccc ctctcccacc
     1201 tgggtgaaga gggcctccgc tccttcgcaa taggtattct gatcggcatt gacgatggcg
     1261 atggggaacg
//
LOCUS       DAOQOC010001077         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_97252_length_1270_cov_3.818930, whole genome shotgun sequence.
ACCESSION   DAOQOC010001077 DAOQOC010000000
VERSION     DAOQOC010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97252_length_1270_cov_3.818930"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..>1270
                     /locus_tag="PL106_14540"
     CDS             173..>1270
                     /locus_tag="PL106_14540"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNA34342.1"
                     /translation="MRSFLVTTVAALALAATASAQPYRFGCHYFRNASYHPHRPQLIG
                     VREGILDVIARSDTFDILHYDIALDITDVGGHTIKAATGVTYTALVADAPLRLDLIDA
                     LTVDSVTSSGGPLPFTHADSLLTIVLGPTAIGDTGTITVHYHGIPGYDTDWGGFRYQN
                     GYIYNLGIGLSTIPPNFGKVWYPCFDSFVERATYAYHVKSAGTYRAHCQGQFLGETQL
                     GGDTVVRSFALDHAIPTHISAIAAANYADSNWTHTGAYGDIPVRLTAKPAALAGMVSK
                     FASLGDAIDCYEFWYGPYPYDRVGYALTTDGALEIAENIAYPDFMPEQSLFDNRGLFG
                     HELGHHWWGDNVTPRIHNHMWLKEGPAEYSGH"
BASE COUNT          246 a          441 c          373 g          210 t
ORIGIN      
        1 catcacagcg aagggcagca aggccaggat ccaggcgaac tgagcgggga ccgggcggag
       61 gcccgtacca agtggccgat cccgcggatc acggtgatcg gggtccggcg aagcgccccg
      121 gggccgtggg tgtgcggctt cgttacattc ggcgcccacg cagcttcgac acatgcgatc
      181 attcctcgtg accacggtag cggccctcgc actggccgcc accgccagcg cccagcccta
      241 ccgcttcggt tgccactact tccgcaacgc atcgtaccat ccgcatcggc cacaactgat
      301 cggcgtccgc gaaggcatcc tcgacgtgat cgcgcgcagc gacaccttcg atatcctgca
      361 ctacgacatc gcactggaca tcaccgatgt gggcggacac acgatcaagg cggcgaccgg
      421 cgtgacatat accgcactgg tggccgatgc gcccctccga ctggacctga tcgatgcgct
      481 caccgtggat tcggtcacct catccggcgg tccactgccc tttacccatg cggacagcct
      541 gctcacgatc gtcctgggcc ccacggccat cggggatacg ggcaccataa cggtacacta
      601 tcacggcata cccggatatg ataccgattg gggcggcttc cgataccaga acgggtacat
      661 ttacaacctc ggcatcggtc taagcaccat cccgcccaac ttcggcaagg tctggtaccc
      721 gtgcttcgac agctttgttg aacgtgccac ctatgcatac cacgtgaaaa gcgcaggcac
      781 ctaccgggcg cactgtcagg gccagttcct gggtgagacc caactcggcg gcgatacggt
      841 ggtgcgttcc ttcgccctgg accacgcgat ccccacgcac atctcggcca tagcggcggc
      901 caactacgcg gacagtaact ggacgcacac cggcgcgtac ggcgacattc ccgtgcgcct
      961 cacggcgaag cccgcggccc tcgccggcat ggtgagcaag ttcgccagcc tgggcgatgc
     1021 catcgactgc tacgaattct ggtacggccc gtacccgtac gaccgtgtgg gctacgcgct
     1081 caccaccgac ggtgcgttgg agatcgccga gaacatcgcc tatccggact tcatgcccga
     1141 gcaatcgctg ttcgacaacc gcggtctgtt cggccacgaa ctgggccacc attggtgggg
     1201 cgacaacgtg accccgcgca tccacaatca catgtggctg aaggaagggc cggccgagta
     1261 cagcgggcat
//
LOCUS       DAOQOC010001078         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_97271_length_1270_cov_3.302058, whole genome shotgun sequence.
ACCESSION   DAOQOC010001078 DAOQOC010000000
VERSION     DAOQOC010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97271_length_1270_cov_3.302058"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PL106_14545"
     CDS             <1..481
                     /locus_tag="PL106_14545"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNA34343.1"
                     /translation="IRLLSGVIDRTRQYTLQYMEHLQDVDPHHEFVCEGKRLNTLFWL
                     TAHLTATENGLLLLATGGPFERFSWAKHYGPGSTGLPKDQCPPYKEVRATMDAVHERA
                     MTHLPTLEDIVLERPNNTGFLRFGPLMRDVLIQAIRHENYHTGQIGWLCKLYGRKTV"
     gene            609..1226
                     /locus_tag="PL106_14550"
     CDS             609..1226
                     /locus_tag="PL106_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34344.1"
                     /translation="MPAPGSLVLPNGGLTPPALELLHAVTGVPMHLLERARIRPARDN
                     WIRVPWYRYHRGGAMTVGRTIWFTRKWYDAAGYGDGSPLATWNWLAHLAHETGHLPQA
                     ERFGRHILGKARYIMAFAAQYAQRALMLRSDPHDGAPLEFEADHGRWVLLQLVGDRPL
                     AHPLLHALHMRDAQAVRAQCHQCASRIADLSQRYAEHKRKVLLHT"
BASE COUNT          256 a          431 c          366 g          217 t
ORIGIN      
        1 gatccgtctc ctttccggtg tgatcgaccg gacccggcaa tacacgctcc agtacatgga
       61 gcacctgcag gacgtggacc cgcaccacga attcgtctgc gaaggcaagc gattgaacac
      121 cttgttctgg ctgaccgcgc acctgaccgc gaccgagaac gggctgttgt tgctggccac
      181 gggcggaccc ttcgagaggt tcagctgggc caagcactac ggccctggat ccaccggcct
      241 gcccaaggat caatgcccgc cctacaagga ggtacgcgct accatggacg cggtacacga
      301 gcgggccatg acccatctgc cgacattgga ggatatcgta ctggagaggc caaacaatac
      361 cgggttcttg cgattcggcc ccttgatgcg cgatgtgctc attcaagcca tccggcacga
      421 gaactaccat acgggccaga tcggatggct gtgcaaactg tacgggcgga aaaccgtatg
      481 atcccgcaga gcggaccagc ttcaacggac ttgtgacggc ctgacgacct gatccctgat
      541 gatcggccct gggcgcttcg ttcacgccgg gacatctgca agacaccagc ctcgatcgac
      601 cacctcaaat gcccgcccca ggatcgctgg tactgcccaa cggagggttg accccgcctg
      661 cactcgaatt gctgcacgcg gtaacgggcg tccccatgca cctgctcgaa cgtgcccgga
      721 tccggcccgc acgcgacaat tggatccgcg taccatggta tcgttaccat cggggcgggg
      781 cgatgaccgt tggacggacg atctggttca cccgcaaatg gtacgatgcg gccgggtatg
      841 gcgatggctc accactggcc acctggaact ggctggcaca tctggcccat gaaacaggtc
      901 accttccgca agctgaacgg ttcggtcggc acatccttgg caaagcccgt tacatcatgg
      961 ccttcgccgc acagtacgcc caacgcgcgc tcatgctgcg ttccgatccg cacgacgggg
     1021 caccactgga gttcgaagcc gaccacggcc gctgggtgct gctgcaactg gtcggcgacc
     1081 ggcccctcgc ccacccgctc ctgcacgcac tgcacatgcg cgatgcacaa gcggtacgtg
     1141 cgcagtgcca ccaatgcgca tcacgcatcg ctgacctgtc gcaacgatac gcggagcaca
     1201 agcgcaaggt cctcctgcac acctgatcca ctcacggtcg atcaaggctc gccgccggtg
     1261 accccgtgat
//
LOCUS       DAOQOC010001079         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_97593_length_1268_cov_2.804617, whole genome shotgun sequence.
ACCESSION   DAOQOC010001079 DAOQOC010000000
VERSION     DAOQOC010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97593_length_1268_cov_2.804617"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1268
                     /locus_tag="PL106_14555"
     CDS             94..>1268
                     /locus_tag="PL106_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA34345.1"
                     /translation="MHRLLAALLLLRVLLAHGQDASLVPDLRATLATATNDTLRADAL
                     ARICFNLIRSEPDSARLAGEQAMTLAKRIGNQRALGDAHNNLGWLAAEQGQLDRADSL
                     LRIALGIFQKIGVPAYTSVTLSNLGWLAEKRGDSVGALKRFQEALTLSEAAKDTATVS
                     ILLYSIGTSYRKIKDHEQAVLYLERSQAYERLLHRPGKEAHCLVALANTLKERGDTLG
                     AIDRYHEAIAIYKRIPDHLGWGIAEENLGDLFQLRSPRTALEHYGIALAKYDSLESTI
                     DQAYVLQRIGKARLELGDVVGAGSSLNRGLELSSSAGDPALTMEYEMALARLAAKNGD
                     AQHTMSHFDRYLVLKDSIQGADTQRELARLRTEFETERKEKDNIILRAENSEQAARIR
                     "
BASE COUNT          265 a          397 c          389 g          217 t
ORIGIN      
        1 tagaatgccg ccctgtcacg gcggaggtcg cgggttcgag tcccgtccgg tccgctggaa
       61 aaggtcacct tcgggtggcc ttttggcttt tccatgcaca ggctccttgc cgcacttctc
      121 ctgcttcggg tcctgctggc gcacggccag gatgcctcgc tggtgcccga ccttcgcgca
      181 acgctggcca cggccaccaa cgataccctg cgggccgatg cgttggccag gatctgcttc
      241 aacctgatac gctccgaacc ggacagcgcc cgattggccg gtgaacaggc catgaccttg
      301 gccaagcgca tcggcaatca gcgggcgctg ggcgatgccc acaacaacct gggctggctg
      361 gccgccgaac aaggccaatt ggaccgggcg gattccctcc tacggatcgc acttggcatc
      421 ttccagaaga tcggagtgcc ggcctacacc agtgtcaccc tcagcaacct gggctggctg
      481 gccgaaaaac gcggggacag cgtgggcgcc ctcaagcgat tccaagaggc actgaccttg
      541 agcgaagcgg cgaaggacac ggccactgta agcatcttgt tgtactcgat cggcacgtcc
      601 taccgcaaga tcaaggatca cgagcaggcc gtgctgtacc ttgaacgatc gcaggcctac
      661 gagcgcctcc tccaccgtcc gggaaaagag gcccattgcc tggtggccct cgccaatacg
      721 ctgaaggagc gcggggacac gttgggcgcg atcgaccggt accatgaggc gatcgccatc
      781 tacaagcgca ttccggacca cctcggctgg ggcatcgcgg aagagaacct cggtgatctg
      841 ttccaactcc gttcaccaag gacagcactg gaacattacg ggatcgctct tgccaagtac
      901 gacagtctgg agagcacgat cgatcaggcg tacgtgcttc agcggatcgg caaggcgcgg
      961 ctggaactcg gcgatgttgt tggcgccggt tcaagcttga accggggctt agaattgagt
     1021 tcgtccgcag gcgatccggc actcaccatg gagtacgaga tggcattggc ccggctcgca
     1081 gccaaaaatg gcgatgcgca gcacacgatg tcgcatttcg accgctacct cgtgctgaag
     1141 gacagcatcc agggtgctga tacgcaacgg gaactcgcac gtttgagaac ggagttcgag
     1201 accgaacgca aggagaagga caacatcatc ctgcgcgccg agaacagcga acaggccgca
     1261 cgcatccg
//
LOCUS       DAOQOC010001080         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_98032_length_1265_cov_4.651240, whole genome shotgun sequence.
ACCESSION   DAOQOC010001080 DAOQOC010000000
VERSION     DAOQOC010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98032_length_1265_cov_4.651240"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PL106_14560"
     CDS             <1..1014
                     /locus_tag="PL106_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34346.1"
                     /translation="PLLHLLNFLYNDAVPTSPEIIQGYIAALESARKRIGLGVEAGDA
                     LSVAMATNEIDHTLRRMQDDLDATHRAVLAEVARYKADRTSVSVRERYLRILTLMDQY
                     VHPLVEIIRVDGLLESTLMETDMVLRAAREQGVYVELGMVERNERQIRVMRRRAIHTL
                     NESRKELQPLYDVLRRSSAIAHGATLALGRLRQMKLEDWVNMYVTPTTNARIEVPPTE
                     EVLRRIVDEVVNHPPIPPPVLNTTENGDTPPDYVRLLWLNELPNLLSEELPVKDVTGW
                     LTGNFPEKGTSDALLGLSKLLFDPKLDVEFKGGKMKQYRTRDGVLEASAIAINKAGPG
                     ANT"
     gene            1053..>1265
                     /locus_tag="PL106_14565"
     CDS             1053..>1265
                     /locus_tag="PL106_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34347.1"
                     /translation="MEPNTTTPLPQLKDIFDKLRQGAYITHEQGTLHAALAEHYEDYK
                     HYFEPLGLNLVRHPRDFFFFHTEAAEG"
BASE COUNT          265 a          431 c          375 g          194 t
ORIGIN      
        1 cccctgctgc acctgctcaa cttcctgtac aacgacgcgg tgcccaccag cccggagatc
       61 atccagggct acatcgccgc attggagagc gcccgcaaac ggatcggtct cggcgtggag
      121 gccggcgacg cgctcagcgt ggccatggcc accaacgaga tcgaccacac cctgcgccgc
      181 atgcaggacg atctggacgc cacccaccgc gccgtgctgg ctgaagtggc ccgctacaag
      241 gccgaccgta ccagcgtgag tgtgcgcgag cgctacctgc gcatcctcac cctgatggac
      301 cagtacgtgc atccgctggt ggagatcatc cgcgtggacg gcctgctgga aagcaccctg
      361 atggagaccg acatggtgct gcgcgccgcg cgcgaacagg gcgtgtacgt ggaactcggc
      421 atggtggaac gcaacgaacg gcagatccgc gtgatgcgcc gccgtgcgat ccacacattg
      481 aacgagagcc gcaaggaatt gcagcccttg tacgatgtgc tgcgccgttc gagcgccatc
      541 gcccatggtg ccacgctcgc cttgggccgc ctgcgccaga tgaagctgga ggattgggtg
      601 aacatgtacg tgacgcccac caccaatgcg cgtatcgaag ttccgcccac cgaggaggtg
      661 ctccgccgga tcgtcgacga ggtggtgaac cacccgccga tcccgccgcc ggtgctgaac
      721 accacggaga acggcgacac cccgcccgac tatgtgcgcc tgctctggct gaacgaactg
      781 ccgaacctgc tgagcgaaga actaccggtg aaggatgtca ccggatggct cacgggcaac
      841 ttccccgaaa aagggacttc cgatgcgctg ctcggcctga gcaaattgct cttcgacccg
      901 aagctcgatg tggaattcaa gggcgggaag atgaaacaat accgtacccg cgacggggta
      961 ctcgaggcgt ccgccatcgc catcaacaag gccggacccg gcgcgaacac ataatgatac
     1021 cacacttacg caacgctccg gcgtgctgag ccatggaacc gaacacgacc actccgctac
     1081 cgcaactgaa ggatatcttc gacaagctcc gccagggcgc ctacatcacc cacgagcagg
     1141 gcaccctgca cgcggcgctc gccgagcact acgaggatta caagcactat ttcgagccgc
     1201 tgggcctgaa tctcgtgcgc catccccgcg acttcttctt cttccatacc gaggccgccg
     1261 agggc
//
LOCUS       DAOQOC010001081         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_98174_length_1265_cov_1.452066, whole genome shotgun sequence.
ACCESSION   DAOQOC010001081 DAOQOC010000000
VERSION     DAOQOC010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98174_length_1265_cov_1.452066"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PL106_14570"
     CDS             complement(<1..241)
                     /locus_tag="PL106_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34348.1"
                     /translation="MSTPLRTPWYASIVTRITLVFAALLVGTVVLIAWISWQASRAEV
                     ISQARRAMDHTLDVAIDRLRGEERTLYGQRSGPTRC"
     gene            complement(349..1176)
                     /locus_tag="PL106_14575"
     CDS             complement(349..1176)
                     /locus_tag="PL106_14575"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNA34349.1"
                     /translation="MKDLSTGLRGFSRAFGFALRNGMWWLFLVPVLLWLLFAGGIVWA
                     SAGVVDLVSGWLSTWDIAVPVDDRGGLVGLWDDVKSFFSSAREVIVLVVVKLALWFLF
                     GLVGKYIVLILLSPLLAYASERTEEILTGRIYPFRAGQFLKDVGRGILMALRNGGLEL
                     LINVLLWSITLFVAILAPVTVVLLWLVSSWFYGFSMFDYVYERHRLGIRASARAAWER
                     PGMVLANGMLFNLLMNPPFLHWILGPLLSVFCFSVIPVAASIGAVLAWHEAETRGPR"
BASE COUNT          275 a          402 c          412 g          176 t
ORIGIN      
        1 tgcacctggt tggccccgag cgctggccgt acaaggtgcg ctcctcgcca cgcaaccggt
       61 cgatggccac atccagcgta tggtccatcg ctcgccgcgc ctgactgatc acttcggcac
      121 ggctggcctg ccagctgatc caggcgatca gcaccaccgt gcccaccaac aacgcggcga
      181 acaccagggt gatgcgcgtg acaatgctcg cgtaccaagg ggtgcggagc ggtgtgctca
      241 tgatgctgcg gccagggtga cgaaggtatc ggggaccgtg ccgatcagga gaccatcgta
      301 cccgtgactc ctgtccacca ggttgcacgg cgcattaccc ggatcaggtc atcgcggccc
      361 gcgggtttcc gcctcgtgcc atgccagcac cgccccgatg ctcgcggcca cggggatcac
      421 gctgaagcag aataccgaga gcagggggcc gaggatccag tgcaggaagg gcgggttcat
      481 cagcaggttg aagagcatgc cgttggccag caccatcccc ggtcgttccc aagccgcacg
      541 tgcgctggcg cggatgccca agcggtggcg ttcatacacg taatcgaaca tgctgaagcc
      601 gtagaaccag gacgagacca gccacaagag caccacggtt accggggcaa ggatcgcaac
      661 gaacaaggtg atgctccaga gcagcacgtt gatcagcagt tccaagccgc cgttgcgcag
      721 ggccatgagg atgccacgcc caacgtcctt caggaattgt cccgcgcgaa agggatagat
      781 ccggccggtg aggatctcct cggtgcgctc actggcatat gcgagcagcg gtgaaagcag
      841 gatcagtacg atgtatttgc ccaccagccc gaacaggaac cagagggcca gcttcaccac
      901 cacgagtacg atcacttcgc gtgccgagga gaagaagctc ttcacatcgt cccaaagtcc
      961 caccaggcct ccgcggtcgt ccaccggcac cgcaatgtcc caggtggaga gccagccgct
     1021 gaccaggtcc accactccgg cgctggccca cacgatgccg cccgcgaaga gcagccacag
     1081 caggacgggc accaggaaga gccaccacat gccgttgcgc agtgcgaatc cgaacgctcg
     1141 ggagaagccg cgcaggccgg tggagagatc cttcatggcg gaaaggaacg gagttgtgcg
     1201 atccaggtgg caggggcagg agcaggggca ggagcaggag caggggcagg agcaggggca
     1261 ggagc
//
LOCUS       DAOQOC010001082         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_98232_length_1264_cov_3.261373, whole genome shotgun sequence.
ACCESSION   DAOQOC010001082 DAOQOC010000000
VERSION     DAOQOC010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98232_length_1264_cov_3.261373"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PL106_14580"
     CDS             <1..288
                     /locus_tag="PL106_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34350.1"
                     /translation="DSIRSLGFMDVAVREWRTEGDSTYQFRTSVAPRTAVHGDSGAFT
                     FTFSGDSVQVYDDLRGGSEVMYPSGRVGYRSNHGHWRLVIDGEERMSGEGV"
     gene            500..>1264
                     /locus_tag="PL106_14585"
     CDS             500..>1264
                     /locus_tag="PL106_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34351.1"
                     /translation="MDTRIMWGRPLNRLVGVAFVCWALGQAQEVRASHLVGGDLGYQY
                     LGETFPGSGLFRYNLKMRMYLNCGPSSNWPQMIDWLQGAPGLNVGVYEEDPLNPGAAK
                     VRYTVAVVTMTSYSVITPDIPDSCVLGDGQCVEESLFEGEVVVPASTGGYHLYFQGYS
                     RNDAILNLFDPGNAGFGIYSFIPPTAIVNSSPVFSGVPVPFICVTESTSFSSAAEDAD
                     GDSLVFTFEKPYDSQEDIAGIVQPPLVLDWPIAPVVY"
BASE COUNT          235 a          367 c          424 g          238 t
ORIGIN      
        1 gactcgatcc ggagcctcgg tttcatggac gtggccgtgc gtgaatggcg cacggagggt
       61 gacagcacct accagttccg tacgagcgtg gcgccgcgca cagccgtgca tggcgacagt
      121 ggcgcgttca cattcacctt cagcggggac agcgtgcagg tgtacgacga cctgcgcggc
      181 ggttcggaag tgatgtatcc gagcgggcgt gttggctacc gttccaacca tggccattgg
      241 cggctggtga tcgacggcga ggaacggatg agcggggaag gcgtgtgagc acgcagtcac
      301 ccagagccag atcccttggc atgggcacgt atcacctcag cagctccacc ggaccagccg
      361 cgacctgagg acagggttct cggaaccagg tgcgtgtgcg caaggtgtcg aacggaagga
      421 gatgcttggc cgggacaacg gcgaccggag gaccagcgtc ctgctatggg gcaaccacca
      481 atacctatat tgacgggcca tggacaccag gatcatgtgg ggccgaccgc tgaaccgcct
      541 cgtgggcgtt gcattcgtct gctgggccct gggacaggca caagaggtgc gggcctcgca
      601 tctggtgggt ggcgatctgg ggtatcaata cctgggcgag accttcccgg gcagcggcct
      661 gttccgttac aacctgaaga tgcggatgta cctgaactgc ggccccagct ccaactggcc
      721 ccagatgatc gattggttgc agggggctcc gggattgaac gtcggggtgt acgaggagga
      781 tccattgaac ccgggtgcgg ccaaggtcag atatacggtc gccgtggtaa cgatgacctc
      841 gtacagcgtg atcacacccg acattccgga cagttgcgtc ctgggcgacg ggcagtgtgt
      901 ggaggagagc ctgttcgaag gcgaagtggt ggtaccggcg agcacgggtg gctaccacct
      961 gtacttccag gggtattcgc gcaacgacgc gatcctgaac ctgttcgacc ccgggaacgc
     1021 gggcttcggt atctattcct tcattccacc gaccgccatc gtgaactcct cccccgtctt
     1081 ctccggtgtt ccggttccct tcatctgtgt gaccgagagc acctcgttct ccagtgctgc
     1141 cgaggatgcc gacggggact cgctggtgtt caccttcgaa aagccgtacg attcgcagga
     1201 ggatatcgca gggatcgtac agccaccgct ggtcctggac tggccgatcg caccggtggt
     1261 gtac
//
LOCUS       DAOQOC010001083         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_98506_length_1263_cov_1.766556, whole genome shotgun sequence.
ACCESSION   DAOQOC010001083 DAOQOC010000000
VERSION     DAOQOC010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98506_length_1263_cov_1.766556"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..>1263)
                     /locus_tag="PL106_14590"
     CDS             complement(302..>1263)
                     /locus_tag="PL106_14590"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34352.1"
                     /translation="EFDHDGPGFSPSQQVIQAQVCPAVPKIALVFDHTHMHGHFLREF
                     RAVPQGPHGDLSRHRGAIASNHDKGLSHWKVHTFHGIHLLNILPETTAAELKKHGSAQ
                     ARSYADCTVLFSDFQGFTVLSERYEATGIVTELDRFFRAFDRITDKYGTEKIKTIGDA
                     YMCASGVPEPSPTHALDAVLMALEMLNVVDHINAQRISEGDPAWPIRIGLNSGPLIAG
                     VVGEKKFAYDIWGDTVNLASRMESNGAAGRVNVTGPVMRQVDAYVISEPRGKVNIKGK
                     GEVESWFIDRLRPEHSADERGRTANAALMDLRKGLRIRHTISQ"
BASE COUNT          202 a          364 c          418 g          279 t
ORIGIN      
        1 ggttgtacag ggtcgtgttc atggtcggtc gtcctttctt ggcatgaaga cgttggcttg
       61 ggcgaatagt ggcctggctc gcttgtgaaa ttgtggtaaa gcgccgttgc gcaggcgctc
      121 atggcccgac ggccgcttgg ggcgggccgt tgctaatacc atttcgcgat aggaggtcgt
      181 ccactgatgt gctgctgatt attcggaggg cgatacgtgg gagtcgttgc gtagtggggt
      241 ctacggaacg acccgcttgg agaagccatg cggaaaatga gcaagcaggc tggacgatgt
      301 tctattgcga gatggtatga cggatccgca agcccttccg caggtccatc aacgcggcgt
      361 tcgccgtgcg cccgcgttca tcggcgctgt gttcaggccg aagccggtcg atgaaccagc
      421 tttccacctc gcctttgccc ttgatgttca ccttgccgcg tggctcgctg atcacgtagg
      481 catccacctg gcgcatcacg ggtcccgtca cattcacacg gcctgccgcg ccgttgcttt
      541 ccatgcggct ggccaggttc acggtgtcgc cccagatgtc gtacgcgaat ttcttctcgc
      601 ccaccacgcc ggcgatcagc gggccgctgt tcagcccgat gcggatgggc cacgccggat
      661 cgccctcgct gatgcgttgc gcattgatgt ggtccaccac gttgagcatc tccagggcca
      721 tgagcaccgc atccagggcg tgtgtggggc tgggttcggg aacaccgctg gcgcacatgt
      781 acgcatcgcc gatggtcttg atcttctccg tgccgtactt gtccgtgatg cggtcgaagg
      841 cccggaagaa gcggtccagt tcggtgacga tacctgttgc ctcgtatcgt tcgctcagca
      901 cggtgaagcc ctggaagtcg ctgaagagca cggtgcagtc cgcgtagctc cgcgcctgtg
      961 ccgagccgtg cttcttcagt tccgcggccg tggtctcggg caggatgttc aacaggtgta
     1021 tcccgtggaa ggtgtggacc ttccagtggg agagtccctt gtcgtggttg gacgctatcg
     1081 caccgcggtg ccgagagagg tcaccgtgcg ggccttgtgg aacggcacgg aactcacgaa
     1141 gaaagtgccc gtgcatgtga gtgtggtcga agaccagggc gatcttcggt accgctgggc
     1201 aaacctgcgc ttggatcacc tgctgactcg gggagaatcc cgggccgtcg tggtcgaact
     1261 cgg
//
LOCUS       DAOQOC010001084         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_98542_length_1262_cov_3.957746, whole genome shotgun sequence.
ACCESSION   DAOQOC010001084 DAOQOC010000000
VERSION     DAOQOC010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98542_length_1262_cov_3.957746"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PL106_14595"
     CDS             complement(<1..1096)
                     /locus_tag="PL106_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34353.1"
                     /translation="MKHVVLLITTLLIALHGNAQQRGAWTRLTAPAKPMHLAAHHPDR
                     GNAPANDDCANAEAITVTTDCASPVSGNNAEATQDGGIPSCDNTGTWLDVWYTFNSGA
                     ADTVTITLTPGAGMTDRVLAVYDACGGDELLCAIAPPGPVALDVEPGMDYWIRVHSNT
                     DYGVGGDFTLCVTVGNTTPAPPVNDLCSGAIEQALSIGGTITFNGDNTGATDSEGLGY
                     TSAWEAFTLAACADVHLSYCGSTPAWDGFWILLYSSCPPSTAVEVSSYDSTSCGDGNF
                     TLCFPQLPAGTYYFPVVAGPIGPYTLVVSAAACGSDQAPNDECDGAIPLTAQPTCDPT
                     YFTNPCATQSLAAVTCGSFTGDAGDDVWYSF"
BASE COUNT          239 a          362 c          420 g          241 t
ORIGIN      
        1 tgaagctgta ccatacgtca tccccggcat cgccagtgaa cgagccgcag gtgacagcag
       61 ccaggctctg cgtggcgcac gggttcgtga agtaggtcgg gtcgcaggtc ggttgtgcgg
      121 tcaacggaat ggccccgtcg cattcatcgt tcggcgcttg atcggagcca cacgcggcgg
      181 ccgagacgac cagggtataa gggccgatcg gtccggcaac gacggggaaa tagtatgtgc
      241 ccgcgggtaa ttggggaaag cacagcgtga agttgccatc gccacaggac gtgctgtcat
      301 aagagctcac ctccaccgcc gttgagggcg ggcaactcga gtacaagagg atccagaaac
      361 cgtcccaggc aggcgtgctc ccgcaatagc tgaggtgcac atccgcgcaa gcggccagtg
      421 taaaagcctc ccaagcggac gtatagccca atccttccga atccgttgct cccgtattat
      481 ccccgttgaa tgtgatcgtt ccaccgatgg agagggcctg ttcgatcgcg ccggagcaga
      541 ggtcgttaac cggaggtgct ggtgtggtgt tcccgaccgt tacgcacaag gtgaaatccc
      601 cgcctacgcc atagtcggta ttggaatgta cgcggatcca atagtccata ccgggctcca
      661 catccaaggc gacggggccg ggtggagcga tagcgcagag gagttcatcg ccgccgcagg
      721 catcgtatac ggccagcacg cggtcggtca ttccagcgcc gggcgtcagg gtgatcgtta
      781 ccgtatccgc tgcgccactg ttgaacgtgt accacacgtc caaccaagtg ccggtgttat
      841 cgcacgaggg aatgccaccg tcctgggtcg cttccgcgtt attcccgctc accggcgaag
      901 cgcaatccgt cgtgacggtg atcgcttcgg cgttggcaca atcatcgttc gcgggtgcgt
      961 tgccgcggtc ggggtggtgc gccgcgaggt gcatcggctt ggcgggtgcg gttagacgcg
     1021 tccaggcgcc ccgttgctga gcgttcccgt gcagcgcgat gagcagggta gtgatgagga
     1081 gtacgacgtg tttcatgggg tcggcgatgt tcggtacttg gatgtggcaa acgttcagcg
     1141 atccggatgg tggcgaaacc ccatgaatgg ggggctcacc gcttcacgaa gcggtgcgcc
     1201 gagtgttcgt tcccgttggt gatccggaac aggtacgtcc cgggtacgag cgatcgcaca
     1261 tc
//
LOCUS       DAOQOC010001085         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_98675_length_1262_cov_1.729080, whole genome shotgun sequence.
ACCESSION   DAOQOC010001085 DAOQOC010000000
VERSION     DAOQOC010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98675_length_1262_cov_1.729080"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PL106_14600"
     CDS             <1..191
                     /locus_tag="PL106_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNA34354.1"
                     /translation="PSVVFTLLSLIIAIIPFTRFMHFLVAPIDYLWRGYQLVIWNWGR
                     RAIRSSGSYYPGMRSKNN"
     gene            259..963
                     /locus_tag="PL106_14605"
     CDS             259..963
                     /locus_tag="PL106_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNA34355.1"
                     /translation="MKIIDTRTGKRVGQEEQAIKKKLKEEDPIKRNVEKETGQAELSP
                     MEPPGAYEANVPEAADAPVRHPLLVQYMREHEKAREVLDRFEKAMVDYHQLGFKLNDA
                     INDCLREFFTFYEETLFDHNQREERQLFPLLHERLIASGEHSESKPPTTAVDMMEDDH
                     VKFIQLSALTFNLLGLAPRLPDERSRGFVFQTAFNNARELVELIRLHIYREDHVVFPL
                     AQKLISNKELDAMMHP"
     gene            978..>1262
                     /locus_tag="PL106_14610"
     CDS             978..>1262
                     /locus_tag="PL106_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNA34356.1"
                     /translation="MTHTELIDRFGRRHDYLRISLTERCNLRCVYCMPAEGIMLRPRE
                     QFMRAEEVIVMARMFTDLGVKKIRLTGGEPLVRKDAAQIITELGRLPVELA"
BASE COUNT          268 a          374 c          387 g          233 t
ORIGIN      
        1 tcccgtccgt tgttttcacc ttattgtcct taatcatcgc catcatcccc ttcacccgct
       61 tcatgcactt cctggtggcg ccgatcgact acctgtggag gggttaccag ctggtgatct
      121 ggaattgggg ccggcgggcc atccgcagct caggcagcta ctatcccggc atgcggtcga
      181 agaacaattg aggagcgata gcgcaacccg gccctgcggc cggaggggag gtcggaggcg
      241 ctgctttaac ttcgtgccat gaagatcatc gatacgagaa cggggaaacg ggtgggccag
      301 gaggagcagg ccatcaagaa gaagctcaag gaggaggacc cgatcaagcg caacgtggag
      361 aaggaaacgg gtcaggccga actttcgccc atggagccgc ccggagccta cgaggccaac
      421 gtacccgaag cggccgatgc gccggtgagg catccgttgt tggtgcagta catgcgcgag
      481 catgagaagg cccgcgaggt cttggaccgt ttcgagaagg ccatggtgga ctatcaccaa
      541 ctgggcttca aactgaacga cgcgatcaac gactgcttgc gggagttctt cacgttctat
      601 gaggaaaccc tgttcgacca caaccaacgt gaggaacggc agttgttccc cctgttgcac
      661 gaacgcctga tcgcatctgg cgagcacagt gagagcaaac cgcccaccac ggccgtggac
      721 atgatggagg acgaccatgt gaagttcatc cagctcagtg cgctcacctt caacctgctc
      781 ggtctggcgc cacgcctgcc ggacgaacgt tcgcgcggct tcgtgttcca gaccgccttc
      841 aacaatgcgc gggaacttgt ggaactgatc cgcttgcaca tctaccgcga ggaccatgtg
      901 gtgttcccgc tggcgcagaa gctgatcagc aacaaggaac tggatgcgat gatgcatccc
      961 tgacctgccc ggccatcgtg acgcacacgg agctcatcga tcgtttcggc cgcagacacg
     1021 actatttgcg gatctcgctc accgagcgct gcaacctgcg ttgcgtctac tgtatgcccg
     1081 ccgaaggcat catgctgcgg cctcgagagc agttcatgcg tgcggaggaa gtgatcgtca
     1141 tggccaggat gttcacggac ctgggtgtga agaagatccg actcaccggc ggcgaacccc
     1201 tggtgcggaa ggacgcggct cagatcatca cggaactggg ccgcctgccc gtggaactgg
     1261 cc
//
LOCUS       DAOQOC010001086         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_99055_length_1259_cov_3.729236, whole genome shotgun sequence.
ACCESSION   DAOQOC010001086 DAOQOC010000000
VERSION     DAOQOC010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99055_length_1259_cov_3.729236"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PL106_14615"
     CDS             complement(<1..699)
                     /locus_tag="PL106_14615"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HNA34357.1"
                     /translation="MTSTGEGPTAWFQDENMAVQYENAAYGLRQNFVVFTPPVGDGAL
                     EVMLDVRGTLLPSVDPDGLGLTFKDDAGLNALTYRDLRCWDALQRPLQAQMILSGAAG
                     EHRLALRVNDREAVYPITIDPVSTSPNTLLTGPVLNQQYGLSVCTAGDLNGDGRSDVV
                     IGAPYTLVGGGGNTGRVYVHYGTNSGITTVPSLVLNGPQAGGLYGSAVSTAGDVNGDG
                     FSDLLIGARSYESNV"
BASE COUNT          235 a          382 c          399 g          243 t
ORIGIN      
        1 cacgttgctc tcgtacgacc gtgcgccgat gagcaggtcg ctgaatccgt ccccgttcac
       61 atcacccgcg gtgctgaccg ccgagccgta caggccacct gcctgagggc cgttgaggac
      121 gaggcttggc accgtggtga tgccgctgtt ggtgccatag tggacgtaca cccggccggt
      181 attcccacct cctccaacaa gggtgtacgg cgcgccgatg accacatcac tacgaccatc
      241 gccgttgagg tcgcccgccg tgcatacgga gagaccgtac tgctggttga gcacggggcc
      301 tgtgaggagt gtgttgggtg aggtggatac cggatcgatg gtgatcggat acacggcctc
      361 gcggtcgttc acccgaaggg ccaggcggtg ttcacctgcg gcaccggaga ggatcatttg
      421 agcctgtaac gggcgttgaa gcgcatccca acaacggagg tcccggtagg tgagcgcatt
      481 caggccagca tcatccttga aggtcaatcc gagtccgtcg gggtcaacgc tgggcagcaa
      541 cgttccgcgc acatccagca tcacctcgag tgctccatcc cctacgggag gcgtgaacac
      601 gacgaagttc tgtcttagcc cgtacgctgc gttctcgtat tgcacggcca tgttctcgtc
      661 ctggaaccaa gcggtagggc cttcaccagt gctcgtcaaa catgtccaag gctcggctgc
      721 tcctccatgg cgcccgacgg cggaaacggc aaaacgtacc tgcacctggt catcatctct
      781 gaatagcgcg aaaccctgtt ccgtgatcac ggcatggtat ccaccgggaa tatggtcagc
      841 cagcaacgcc gaccggcctg ttgcgggtcg aagatcccat gcgcccgtac ctgggggtgt
      901 ggttccgaaa gacaggctgg cggaaaagaa caagaaggca ctcaccagtc ggctgctgcg
      961 actggcccga ccacctgttc ggttggcgcg agcgtacggt ttcatacggg gggctggagc
     1021 aggcccaagg agcatgcgca agttatggaa cgacttttaa cgcggatacc ttcgccgggt
     1081 tgctttccgg tcacatgcac agctcgggct ccatcaggcg accaggtctc acgtgggtct
     1141 gctggatcgg agtattagtt ggcgcccatg ggcagtcggt ggctccagcg gacagtacac
     1201 ttgagcctgt cccggtggcc cgccgttggg gtttggccgc aggcagcgct gccgtgatc
//
LOCUS       DAOQOC010001087         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_99086_length_1259_cov_2.950997, whole genome shotgun sequence.
ACCESSION   DAOQOC010001087 DAOQOC010000000
VERSION     DAOQOC010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99086_length_1259_cov_2.950997"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PL106_14620"
     CDS             complement(<1..1186)
                     /locus_tag="PL106_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34358.1"
                     /translation="MRQLIALGDSMLWVVTEAGLYRYDVSRGAFATEQGPAPGRGPHG
                     MIDAASALHWNEAARTLWAGTHKGLAAWDARTDTWTDHRNGRGPLWRDSTGTDAPVVH
                     DSALYFLRNKPYTLFAYDLRAHELRAQPDVEASPNHFTLRCQAFDADGRHWLATWTHR
                     LFVREPDGAWQELTADAVNLISSRTGCMLLTRTGEQWLGTDKGIVVLRRSATAFRSLA
                     HDLGTAHISAMRLWGTDTLLVGTAGDGVRICSMKTGSCTGLRSDHTSSVDESEWRANS
                     IQGFSSDADGTVLVCTSNGLLELDPRALTLRPAAVLMDAIKRGRARAYTVAERTEGAL
                     WLGTWSSGLWRVDPGTGAGELVDTAEGRWGKLPGRLILCWLNGRDGTVWLGLNDGGGL
                     ARM"
BASE COUNT          209 a          453 c          385 g          212 t
ORIGIN      
        1 gcatgcgcgc gagtcctcct ccgtcgttca acccgagcca caccgtgcca tcgcgcccat
       61 tcagccagca caggatcaag cgcccgggca gcttgcccca tcggccttcg gccgtatcga
      121 ccagctcgcc tgcgccagtg cccggatcca ctcgccacag tccgctcgac caggtgccca
      181 gccaaagcgc gccttccgtt cgctccgcaa cggtgtacgc ccgtgcccgc ccccgcttga
      241 tcgcgtccat caacacggcc gcggggcgta gcgttagcgc gcgcgggtcc aattccaaca
      301 ggccattgct cgtacacacc agcactgttc cgtcggcatc gctgctgaac ccctgtatgg
      361 aattcgccct ccactcggac tcgtccaccg aggacgtgtg atcgcttcgt agcccggtac
      421 agctaccggt cttcatggaa catatccgca ccccatctcc cgccgtaccg accaacaggg
      481 tgtccgttcc ccacagccgc atggccgaga tgtgcgccgt gccgagatcg tgcgcgagcg
      541 atcggaaagc cgttgcactg cgccggagca ccacgatgcc cttgtccgtg ccgagccact
      601 gctcgccggt gcgcgtcagc agcatgcagc cggtgcggga agagatcagg ttcacggcat
      661 cggccgtcaa ctcctgccac gccccgtccg gctcgcgcac aaaaaggcga tgcgtccagg
      721 tggccagcca atggcgcccg tccgcatcga acgcctggca tcgcagcgtg aagtgattgg
      781 gactcgcctc gacatccggt tgcgcgcgga gttcgtgagc gcgcagatcg taggcgaaca
      841 gcgtgtaggg cttgttgcgc aggaaataca gcgcgctgtc gtgtaccacc ggcgcatcgg
      901 tgccggtgga atcgcgccac agcggacccc tgccgttgcg atggtcggtc catgtatcgg
      961 tgcgcgcgtc ccatgccgcg aggcctttgt gcgtgccggc ccagagcgtc cgtgccgctt
     1021 cgttccagtg caaggccgag gcggcatcga tcatgccgtg cggcccacgg cctggggccg
     1081 ggccctgctc cgtggcgaat gctccgcgcg atacgtcata gcggtacagg cccgcctcgg
     1141 taacgaccca gagcatcgaa tccccgagcg cgatcaactg gcgcatacga ttggcctggc
     1201 gatcgagcgc accggttgcc gtaacccgcg tattgaagaa ggcacccgtg cgtggatcg
//
LOCUS       DAOQOC010001088         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_99128_length_1259_cov_2.378738, whole genome shotgun sequence.
ACCESSION   DAOQOC010001088 DAOQOC010000000
VERSION     DAOQOC010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99128_length_1259_cov_2.378738"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PL106_14625"
     CDS             complement(<1..1005)
                     /locus_tag="PL106_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34359.1"
                     /translation="MPALLKGPLLTERSARRWGWAIITVLVLVVHWPLSTFTCAMGGG
                     DAFDCWLPWRHFMTLALQNGELPLWNPLQQMGYPVHADLQGPMWYPEAIAIGGTIGHS
                     VITLQVLYLFYVIIGGVGLMRLCNTVHGDARVGVIAGIAYALSGNMVAHQMHFYAIIS
                     AAWMPWFLAAQLRLLRAPAWRRSLEAALFLMLLLTGGNHTFTIMSAYLLLPLLLLHAM
                     RAWHHGRARALRQLLGHQALFVGSTLVMACGTFHALIEVSPYLGRMHGLPYLLVSQEP
                     FTVEALSSLLAPVVISGKNPDLGTDPAMANGYMGVIILALALVSVLRHRSAEENVFAV
                     F"
     gene            complement(1010..>1259)
                     /locus_tag="PL106_14630"
     CDS             complement(1010..>1259)
                     /locus_tag="PL106_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34360.1"
                     /translation="DGTPLSDRPADSGPRKVARADTTALDREPQLSIFQLDDPALEGI
                     RAQLERIDIDTLTPVEALLKLNEIKRMAGVRPAKLKKA"
BASE COUNT          251 a          395 c          407 g          206 t
ORIGIN      
        1 gaacacggcg aacacgttct cctcagcgga ccggtgccga agcaccgata cgagcgcaag
       61 agcaaggatg atcacgccca tgtacccgtt cgccatggcc gggtccgtgc ccaggtccgg
      121 gttcttgcca ctgatgacca ccggggccag caaggaggag agtgcctcca cggtgaaagg
      181 ttcttgcgaa acgagaaggt acggcagtcc gtgcatccgt cccagatagg gcgatacctc
      241 gatcaaggca tggaatgttc cgcaggccat caccagcgtg gacccgacga agagggcctg
      301 gtgtcccaga agctgtcgca gtgcgcgtgc ccgaccgtgg tgccaggccc tcatcgcatg
      361 gagcaacagg agcggtaaca gcagataggc cgacatgatc gtgaaggtgt gattgcctcc
      421 ggtgagcaga agcatcagga acagtgcggc ctccaacgat cgcctccagg caggggcgcg
      481 cagaagccgg agctgtgcgg ccaggaacca gggcatccag gcggcgctga tgatcgcgta
      541 gaagtgcatc tggtgcgcca ccatgttccc gctcaaggcg tacgcgatgc ccgcgatcac
      601 gcccacgcgc gcatcaccgt gtaccgtgtt gcacaagcgc atgaggccga cccccccgat
      661 gatcacatag aacaagtaga gcacttgcaa ggtgatcaca ctgtggccga tggtgccacc
      721 gatcgcgatc gcctccgggt accacatcgg cccctgcaga tcggcatgca cgggatagcc
      781 catctgttgc agggggttcc aaaggggcag ctcgccgttc tgcagggcga gcgtcatgaa
      841 atgccgccac gggagccagc agtcgaatgc atcgcctcct cccatggcgc aggtgaaggt
      901 gctcaatggc caatgcacca cgagcacgag gaccgtgatg atggcccagc cccatcggcg
      961 tgcggaccgt tccgtgagca gcgggccctt cagcaaggcg ggcatgtgct caggccttct
     1021 tgagcttcgc agggcgcacc ccggccatcc gcttgatctc gttcaacttc agcaaggcct
     1081 ctaccggcgt cagggtatcg atgtcgatgc gctccagttg tgcacggatg ccctccaggg
     1141 cagggtcgtc caactggaag atgctcaatt gcggttcgcg gtccagtgcg gtggtatcgg
     1201 cccgggccac cttgcgcggg ccgctgtccg caggccggtc cgagaggggt gtgccatcc
//
LOCUS       DAOQOC010001089         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_99631_length_1256_cov_2.205662, whole genome shotgun sequence.
ACCESSION   DAOQOC010001089 DAOQOC010000000
VERSION     DAOQOC010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99631_length_1256_cov_2.205662"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PL106_14635"
     CDS             complement(<1..395)
                     /locus_tag="PL106_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34361.1"
                     /translation="MSAAKSSPGMPAAAARLFIRREYAAIKGQRSSVFWALYAILLCS
                     LLAIVFGKAALDHLHERMEDPFTTTISIPVQNGRYQDRYAHIKAYLDSCAATGAFGVE
                     ASSGDRTASWRVFSPSARQDLFVYVRSFS"
     gene            complement(392..1126)
                     /locus_tag="PL106_14640"
     CDS             complement(392..1126)
                     /locus_tag="PL106_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA34362.1"
                     /translation="MVPAFRLRDLVLGYGGRAVLESGVLDIPPGKVTCVIGPSGTGKS
                     TLLETIGLMSDTFLSTTGPEAAFAIEGEDLDPRTLWATGRERLAAIRRERFSFIFQST
                     NLMPNFTIGENIRVAAHGTGADGALEDRLAVLLDAMRLPRNVLDKPAHAVSGGQKQRI
                     AFIRALVKDFSILLADEPTGNLDPDNSVALFSVLKAHLAERGRTAVIVTHHLDLAETF
                     GDLVLAIEPRGEGPAAITVRKRPVAS"
BASE COUNT          229 a          393 c          418 g          216 t
ORIGIN      
        1 aaggagaagc tgcgcacata gacgaacagg tcctgccggg cgctgggcga gaacacccgc
       61 cagctcgccg tacggtcgcc ggaactggcc tcgaccccga aggcccccgt ggccgcacaa
      121 ctgtccagat aggccttgat gtgcgcgtac cggtcctgat agcggccgtt ctgtacggga
      181 atggagatgg tggtggtgaa ggggtcctcc atgcgctcat gcaaatggtc caacgcggcc
      241 ttgccgaata cgatggcgag cagtgaacaa agcaggatgg cgtacaaggc ccagaaaacg
      301 gatgaacgct gacccttgat ggccgcatac tcacgccgga tgaaaaggcg cgcggcggcc
      361 gcgggcatcc ccggggagct cttggccgcg ctcatgatgc gacagggcgt ttgcgcacgg
      421 tgatcgcagc cggtccttcg ccacgaggtt cgatggcgag caccaggtcg ccgaaggtct
      481 cggccaggtc cagatggtgc gttacgatca ccgcggtccg gccgcgctcc gccagatgcg
      541 ctttcagcac ggagaacagg gctactgagt tgtcgggatc aaggttgccc gtgggctcat
      601 cggccagcag gatggagaag tcctttacca gggcacggat gaaggcgatg cgctgcttct
      661 gtccacccga caccgcatgc gcgggtttgt ccagcacgtt ccggggcagg cgcatcgcat
      721 ccaacagcac ggccagccgg tcctccagcg ccccatcggc cccggtgcca tgcgccgcca
      781 cccggatgtt ctcgccgatg gtgaagttgg gcatcaggtt ggtgctctgg aaaatgaagc
      841 tgaaccgctc ccggcggatg gctgccaggc gctcgcgacc ggtggcccac agggtgcgcg
      901 ggtccaggtc ctccccctcg atagcgaagg ccgcttcggg gccggtcgtg gacaggaagg
      961 tgtcggacat caggccgatg gtctccagca aggtgctctt gcccgtgccc gagggaccga
     1021 tcacgcaggt gacttttccg ggcgggatgt ccaggacccc gctttccagc acggcccggc
     1081 ccccatatcc cagcacgaga tcgcgcaagc ggaaggccgg taccatggct caattgaaca
     1141 cgtactcgat gggcacccag tagaacttgt tgtgcccctc gggtgcttcg tagtagtatt
     1201 tctggtcgcg cagggaggtg agttccttca cgctctgctg caccagctgc tggtac
//
LOCUS       DAOQOC010001090         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_99803_length_1255_cov_2.184167, whole genome shotgun sequence.
ACCESSION   DAOQOC010001090 DAOQOC010000000
VERSION     DAOQOC010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99803_length_1255_cov_2.184167"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>1255)
                     /locus_tag="PL106_14645"
     CDS             complement(164..>1255)
                     /locus_tag="PL106_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA34363.1"
                     /translation="NNYLGLANHPENRKVDAEAAAAWGMAYPMGARMMSGQTKYHEQL
                     ENELSAFMGKEDTFLLNYGYQGCMSAIEALLGRHDVLVYDSECHACMIDGARLHMGKR
                     FVFQHNDMASLDKQLENAAKLTKETGGGILVMTEGVFGMAGDQGKLKEIVERKAKYNF
                     RLFVDDAHGFGMMGREGRGTADAQGVQDGVDVYFGTFAKAMATIGAFVSGEEDVIMYL
                     RYNMRSQTFAKSLPMPVVIGALKRLEMIRTRPEFSQKLWEITRALQSGLREAGLDIGV
                     TNSAVTPVMMHGSIPEATNVVLDLRERHGIFCSIVVYPVVPKDTILLRLIPTAVHSLD
                     DVKRTIESFKDVKQKLEAGAYKAEKVATI"
BASE COUNT          200 a          419 c          375 g          261 t
ORIGIN      
        1 cgtatcggga cccgtgtgtc ccggtccagc cccaggacaa gtccattttc aagcaattcc
       61 ccctcattca gtccggtcag ttctccggca ctcgttcgcc cgcgcgatac aggaatagcg
      121 ggagcagcgc gacaggccgg gaatgcaggg gtacgaagcg acctcagatc gtggccactt
      181 tctccgcttt gtacgcgccg gcctccagct tctgcttcac atccttgaag ctctcgatcg
      241 tgcgcttcac atcgtccagc gagtggacgg ccgtggggat caggcgcagc aggatggtgt
      301 ctttgggtac tacggggtac accacgatgc tgcagaagat gccatggcgc tcgcgcaagt
      361 ccagcaccac gttggtggcc tcgggtatgc tgccgtgcat catcaccggg gtcacggcgc
      421 tgttggtcac gccgatgtcc agcccggctt cgcgcaggcc gctctgcagg gcgcgggtga
      481 tctcccagag cttctggctg aactccggcc gcgtgcggat catctccagg cgcttgagtg
      541 caccgatcac cacgggcatg ggcaggctct tggcgaaggt ctggctgcgc atgttgtacc
      601 gcaggtacat gatcacatcc tcctcgccgc tcacgaacgc accaatggtg gccatggcct
      661 tggcgaaggt gccgaagtac acgtccacac cgtcctgcac gccttgtgcg tcggccgtgc
      721 cgcggccctc gcgccccatc atgccgaagc cgtgggcatc gtccacgaag agccggaagt
      781 tgtacttcgc cttgcgctcc acgatctcct tgagcttgcc ctggtcaccg gccatgccga
      841 acacgccctc ggtcatcacc aggatgcctc caccggtctc cttggtcagt ttcgcagcgt
      901 tctccaactg cttgtccaag ctggccatgt cgttgtgctg gaacacgaag cgcttgccca
      961 tgtgcagccg ggcgccatcg atcatgcagg cgtggcactc gctgtcgtac accagcacgt
     1021 catgccgacc cagaagggct tcgatcgcac tcatgcagcc ctggtagccg tagttcagca
     1081 ggaaggtgtc ctccttcccc atgaaggcgc tcagctcatt ttcgagctgt tcgtggtact
     1141 tcgtctggcc gctcatcatg cgggccccca tggggtaggc catgccccac gcggcggcgg
     1201 cttcggcgtc caccttgcgg ttctccgggt ggttggccag gcccaggtag ttgtt
//
LOCUS       DAOQOC010001091         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_100042_length_1253_cov_3.740401, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001091 DAOQOC010000000
VERSION     DAOQOC010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100042_length_1253_cov_3.740401"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PL106_14650"
     CDS             <1..805
                     /locus_tag="PL106_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNA34364.1"
                     /translation="NIRRRGEQFTEGELLARAGDRLDPPLIGLLATAGLEEIEVIFKP
                     WVAIVRTGGEFIDDDASKEGRIYSSNDRMLLAALHKELCETDDEGAFTAGDDDAELEI
                     AIRQASAADVVITTGGVSVGDHDRVETVLRKMGARIVFHGVAQKPGKPLLIALLEGKP
                     VFGLPGNPRAVMILYWEYVLPFLRAMQGAPDPWPRTERLPLEHAVDVKGGRAEFRAAR
                     IERGRVRLLVDEGSHMLRSLTAADALVYLPADRRAWHMNDEVEVHYLPR"
     gene            802..>1253
                     /locus_tag="PL106_14655"
     CDS             802..>1253
                     /locus_tag="PL106_14655"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNA34365.1"
                     /translation="MNDRQWTGVVLAGGKSSRMGRDKALIEVDGITLLDRAIDRLRPY
                     TREVIVIADPLKYAPAHARVLPDDRPGLGPLGGIATALRHARYVRLLVLACDLPNIND
                     RLLIQLKNGLVGSVDAVVPRHPDGIEPLAAAYHRHTIECFQGRIADGNL"
BASE COUNT          219 a          389 c          412 g          233 t
ORIGIN      
        1 caacatccga cgtcgtggcg agcagttcac cgagggcgag ttgctggccc gtgccggcga
       61 tcggcttgat cccccgctga tcggattatt ggccaccgcc ggactcgagg agatcgaggt
      121 cattttcaaa ccatgggtgg cgatcgtacg caccggtggc gagttcatcg atgacgatgc
      181 ttccaaggaa ggcaggatct acagcagcaa cgatcggatg ctcctggccg cactgcacaa
      241 ggaactgtgc gagaccgatg acgaaggcgc cttcacggcc ggtgatgatg acgccgaact
      301 ggagatcgcc atcaggcagg ccagcgccgc ggatgttgtg atcaccaccg gtggtgtttc
      361 cgttggcgac catgatcggg tggaaaccgt tctgcggaag atgggggcgc ggatcgtctt
      421 ccacggcgtg gcccagaagc cgggcaagcc gctgctcatc gcactgctcg aggggaaacc
      481 cgttttcggg ctccccggga acccgcgtgc tgtaatgatc ctttattggg agtatgtcct
      541 gccttttctc cgggccatgc aaggggcgcc ggacccatgg ccccgtaccg aacgactgcc
      601 gctggaacat gccgttgacg tgaagggcgg tcgtgctgag ttccgtgccg cacgtattga
      661 acgtggccgt gtgcgcctcc tggtggatga ggggtcccac atgttgcgct ccctcaccgc
      721 tgccgatgcc ctcgtctatc tgccggccga tcgtcgcgcg tggcacatga acgacgaagt
      781 ggaggtccac tacctgccgc gatgaacgac cgccagtgga ccggcgtggt gctcgcgggc
      841 gggaagagtt cgcgcatggg ccgcgataag gcactgatcg aagtggacgg gatcacgctg
      901 ctggatcgtg ccatcgaccg gttgcgcccg tacacccggg aggtcatcgt gatcgctgat
      961 ccgctcaaat acgcaccggc tcatgcccgc gtcctgcccg acgaccggcc cggcctcggt
     1021 ccactggggg ggatcgccac cgctctgcgc catgcgcgct atgtgcgctt gctggtgctg
     1081 gcctgcgacc tgccgaacat caacgaccgg ctgctcattc agctgaagaa cggacttgtt
     1141 ggttcggtgg atgccgtggt gcccaggcac ccggatggta tcgaaccact cgccgcggcc
     1201 taccacaggc acaccatcga gtgcttccag ggcaggatcg cggatggcaa cct
//
LOCUS       DAOQOC010001092         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_100064_length_1253_cov_3.106010, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001092 DAOQOC010000000
VERSION     DAOQOC010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100064_length_1253_cov_3.106010"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..573
                     /gene="ssb"
                     /locus_tag="PL106_14660"
     CDS             136..573
                     /gene="ssb"
                     /locus_tag="PL106_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNA34366.1"
                     /translation="MSGVNKVILIGNLGSDPEIRHLQNDISVANFRIATSETFKDRTT
                     GEKREQTEWHSIVAWRGLANIAEKYLRKGSKVYVEGKLRTRKWEKDGIERYTTEIMAD
                     EMTLLDRPANAPPTAAQPVQRAAGSPEPVTAPAGETESDDLPF"
     gene            604..>1253
                     /locus_tag="PL106_14665"
     CDS             604..>1253
                     /locus_tag="PL106_14665"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HNA34367.1"
                     /translation="MDGPPLPILVAGPPDLAAMIAIAVILVLLVVSACMSASEVALFS
                     LTPTQLRDLKERGGSSGQRVLDLLAKPRRLLATILVVNNFALVGITILSSIVIRGLFD
                     LDHMADYLRFVLQVVLVTFVMLLLGEVVPKVYATGNAMRVAQGMSGPLMTLRTLASPI
                     NEALIRSTSFIEKRYRRRGTQNVGVDALGHALELTKDASTTAGEQKILRGIVKFGA"
BASE COUNT          258 a          385 c          396 g          214 t
ORIGIN      
        1 ccacgctgtc ggccgagggg atcctgttgg gggcggttgg aagaacgggc tggccaaggc
       61 gggtgggcgg tggaaaaacc atcggccagg tccaaggccg tcgccgttat cttcgcaacc
      121 ccaacccatc cagcaatgag cggagtgaac aaagtgatcc tgatcggcaa cctgggttcc
      181 gacccggaga tcaggcatct gcagaacgat atcagcgtgg cgaatttccg catcgccacg
      241 agcgaaacgt tcaaggaccg cacgacaggt gagaagcgtg aacagacgga atggcacagc
      301 atcgtggcat ggcgcggcct ggcgaacatc gccgaaaaat accttcgcaa agggagcaag
      361 gtgtatgtgg aaggcaagct ccggacgcgc aaatgggaaa aggacggcat cgagcggtac
      421 accaccgaga tcatggccga tgagatgacc ttgctggatc gcccggccaa cgcacctcca
      481 accgctgcac agccggtaca acgggcggca gggtctcccg aaccggtgac agctccggca
      541 ggcgaaacag agtcggacga cctgccgttc tgagccgggt ccctttctcc gatcccgcac
      601 ccgttggacg gccctcccct tcccatattg gtcgcagggc ctcccgacct ggcggccatg
      661 atcgccattg cggtcattct ggtactgctc gtggtatcgg cgtgcatgag tgccagcgaa
      721 gtggccctgt tctcgctcac ccccacccaa ctgcgcgacc tcaaggagcg cggcggatcc
      781 agcggtcagc gtgtgctgga ccttctggcg aagccccgcc gactcctggc gaccatcctg
      841 gtggtgaaca acttcgcact tgtggggatc acgatcctgt cctccattgt gatcaggggc
      901 ctgttcgacc tggaccacat ggcggactac ctgcgcttcg tcctccaggt cgtcctggtc
      961 accttcgtca tgctgctgct gggcgaagtg gtgcccaagg tgtacgccac gggcaacgcc
     1021 atgcgcgtgg cccagggcat gagcgggccg ctgatgacgt tgcggaccct tgcgagcccg
     1081 atcaatgagg cgcttatccg gagcacgtcg ttcattgaga aacgatatcg caggagaggt
     1141 acacagaacg tcggagtcga tgcgctgggc catgcgctcg aactcaccaa ggacgccagt
     1201 accacggcgg gagaacagaa gatcctgcgc ggtatcgtga agttcggcgc cat
//
LOCUS       DAOQOC010001093         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_100842_length_1248_cov_5.154233, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001093 DAOQOC010000000
VERSION     DAOQOC010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100842_length_1248_cov_5.154233"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PL106_14670"
     CDS             complement(<1..438)
                     /locus_tag="PL106_14670"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNA34368.1"
                     /translation="MFTRVFKGPDEKIKVGTHRLEALGDGLFAIVMTLMVLELRIPEW
                     EGEVTNARVWHYLAELAPSFFAFALSFIILGIFWFAHRLVHLFIGNSNRRLMWLSMLF
                     YLSISLIPFSAAMLGRYPENQLVEVIYGVNMVAACQFLYWLWNY"
     gene            complement(558..>1248)
                     /locus_tag="PL106_14675"
     CDS             complement(558..>1248)
                     /locus_tag="PL106_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34369.1"
                     /translation="VILRAGAFESGLFAQVHARTDVTKGDFLALFRTRAIALRYLACI
                     AIGLPVWFAVGVLINKSHVIGPAIGVRGVVHTGTSVMWGYIGLSVGDLFSGLLSQWMR
                     SRRKVILLYLAFQTLCVLVLFFQGPMSLEHFYFMCLMIGISTGFWALFVTVASEQFGT
                     NLRSTVTSTVPNFVRGAVIPITLSYVALRGPWGETAAVLITGGVCIGLSLIATLHVRE
                     TFEDDMDFLEG"
BASE COUNT          279 a          369 c          390 g          210 t
ORIGIN      
        1 gtagttccac agccagtaga ggaattggca tgccgccacc atgttcacac cgtagatcac
       61 ctccacgagc tgattctccg gatagcggcc cagcatggcc gcactgaagg ggatcaggct
      121 gatggagagg tagaagagca tgctgagcca catgagcctg cggttgctgt tcccgatgaa
      181 caggtgaacg agccggtggg cgaaccagaa aataccgaga atgataaagc tgagtgcgaa
      241 tgcgaagaag ctcggtgcca attcagccag gtagtgccat acccgtgcat tggtcacctc
      301 accctcccac tccgggatcc gcagttccag caccatcagc gtcatcacga tggcgaacag
      361 gccatcgccc aaggcttcca ggcggtgggt gcccaccttg atcttctcgt cgggaccttt
      421 gaacacgcgt gtgaacatga tcggtggtac gcgaaggtgc gggatcttcc ggtattatga
      481 acgagccctt atcgggcgcc gatcgttggt ccgtgttacc gacgatcgat acatggccca
      541 agggtgcgcc aagttgctca accttccaga aaatccatgt catcctcgaa ggtctcgcgc
      601 acatggagcg tggcgatcag ggacaggccg atgcacacgc ctccggtaat gagcactgcg
      661 gcggtttcgc cccatgggcc gcgcagggcc acgtacgaga gggtgatcgg gatcacggca
      721 ccgcgcacga aattgggcac ggtgctggtg acggtgctac gcaggttggt gccgaactgc
      781 tcgctggcca cggtgacaaa gagtgcccag aagccggtac tgatgccgat catcaggcac
      841 atgaagtaga aatgctccag gctcatcggg ccttggaaga agaggaccag cacacagagg
      901 gtctggaagg ccaggtagag caggatcacc ttgcggcggc tccgcatcca ctggctcaac
      961 aggccactga agaggtcgcc cacgctcagc ccgatatagc cccacatcac gctggtgcct
     1021 gtatgcacca caccccgcac accgatcgcc ggaccgatca cgtggctctt gttgatgagc
     1081 acgcccaccg cgaaccagac gggcaggccg atggcgatgc aggccaggta acgcagtgcg
     1141 atggcacggg tgcggaagag ggcgaggaag tcacccttgg tcacatcggt gcgcgcatgc
     1201 acctgggcga agaggccgct ttcgaacgcg ccggcgcgca ggatcacg
//
LOCUS       DAOQOC010001094         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101003_length_1247_cov_4.168624, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001094 DAOQOC010000000
VERSION     DAOQOC010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101003_length_1247_cov_4.168624"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PL106_14680"
     CDS             complement(<1..384)
                     /locus_tag="PL106_14680"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNA34370.1"
                     /translation="MYEAPKSLRPVIIWLLTGCAMIACMVAIGGITRLTESGLSITEW
                     KPVTGALPPLSEVAWQAEFEKYKKIPEYQLLNQGMDLAGFKRIFFWEWLHRNWGRLMG
                     LVFMVPFLVFWRRGLLKGWLMRRALW"
     gene            complement(581..877)
                     /locus_tag="PL106_14685"
     CDS             complement(581..877)
                     /locus_tag="PL106_14685"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA34371.1"
                     /translation="MKYGLRILPSVTKEARRIHVYREKNQCKGAGGRFMEALGRFYDD
                     ILANPYGHTVRKAPFRHAMLHWLKYRVVYKVEGPVVSVVQVRHTSRKPSKRYGP"
     gene            complement(874..1119)
                     /locus_tag="PL106_14690"
     CDS             complement(874..1119)
                     /locus_tag="PL106_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34372.1"
                     /translation="MDIALKKLDLMQRLMLIWDEAALRRVAKVIEKESPSVVEDEDIT
                     DEEYAAFQEELAKCERGELKFHTREESMRMIREGSKE"
BASE COUNT          256 a          402 c          329 g          260 t
ORIGIN      
        1 ccataaggcc ctccgcatca accagccctt cagcagtccg cgccgccaga acaccaggaa
       61 gggcaccatg aacacgagcc ccatcaaccg gccccagttc cggtgcaacc actcccagaa
      121 gaagatccgc ttgaagccgg ccaggtccat gccctggttc aacaattgat attcaggtat
      181 ttttttgtac ttctcgaact cggcctgcca cgcgacctcg ctcagcggag gcaaagcacc
      241 cgtcaccggc ttccactccg tgatgctgag cccgctctcg gtaagcctgg tgatgccacc
      301 gatggccacc atgcaggcga tcatcgcgca gccggtcagc agccagatga taactggtcg
      361 cagggacttc ggggcttcgt acatgggggc gcgaaactac cgaagcaaga acgggttgat
      421 gtacaccgcc gcccggcaga tccatgcatc cttcttcgtt actgacaata tggcgaacca
      481 gttcaaggac cgcatcaagc accaggaaac cctgcgtgat cgacttgtac tgatcactga
      541 gtggcgagag gagcagaaca agtcggctcc gaccatctgc tcatggcccg taccgcttgc
      601 tgggcttgcg gctggtgtgg cgcacctgta ccaccgagac gaccggacct tccactttgt
      661 agaccacgcg gtatttcaac cagtgcagca tcgcatggcg gaacggggcc ttgcggaccg
      721 tgtggccgta cgggttggcc aagatgtcgt cataaaatcg gcccaaggct tccatgaacc
      781 ggccgcccgc ccccttgcat tggttctttt cccggtacac gtggatccgc cgcgcttcct
      841 tcgtcacact cggaagaatg cgcaagccat acttcattcc ttgctgcctt cacggatcat
      901 gcgcatggat tcctcacggg tgtggaattt gagttcgccc cgttcgcact tggcaagctc
      961 ctcctggaac gccgcgtact cctcgtcggt gatgtcctcg tcctccacaa cagatggtga
     1021 ttccttctcg atgaccttgg ccacgcgccg cagggccgct tcatcccata tcagcatcag
     1081 gcgctgcatc aggtccagct tcttcagtgc gatatccatg gctgcgggtg tttcgtgggg
     1141 taaaaatagc ctgttggggc acgcagccca cgttccagaa aaaccctacg gcccgtaccg
     1201 cttcccaggt tttcggctgg tgtgtatcac acgataaatg aacacat
//
LOCUS       DAOQOC010001095         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101112_length_1247_cov_2.346477, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001095 DAOQOC010000000
VERSION     DAOQOC010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101112_length_1247_cov_2.346477"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PL106_14695"
     CDS             <1..481
                     /locus_tag="PL106_14695"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNA34373.1"
                     /translation="LITGIGFDYRPNDHFSAFVSPATMKLVIVNDPYLWQGITDPETR
                     LYGVKNGNTTEFELGGYIRAQYQTEISKNFTFLTRGDLFCNYLRNPQNIDVTWETLWT
                     LKVNKWFGATLNTLLLYDHDISVPKVRTDESTGAKVSYMGKGTQFKEVLGIGLTFNL"
     gene            837..>1247
                     /locus_tag="PL106_14700"
     CDS             837..>1247
                     /locus_tag="PL106_14700"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNA34374.1"
                     /translation="MAITPYTGPFTDTELRHLLRRTLFGCSNADLDHFAGQTLQQVVD
                     ALLTFSNDTTPPVKTYWLLNGNTPDPTLIDANVPFGNTWINTPRQTADEPTVSGKRIE
                     SLVAWRTGLLAQQDRTLREKLTLFWNNLVPTEAST"
BASE COUNT          281 a          430 c          327 g          209 t
ORIGIN      
        1 cctgatcaca ggtatcggat tcgactatag gcccaacgat catttctcgg cgttcgtctc
       61 ccccgccacc atgaagctcg tgatcgtgaa cgacccctac ctctggcagg ggatcaccga
      121 tccggagacc cgactgtacg gcgtgaagaa cggcaacacc accgagttcg agctcggggg
      181 ttacatacgg gcgcagtacc agacggagat cagcaagaac ttcaccttcc tcacccgggg
      241 cgacctgttc tgcaactacc tccgcaaccc gcagaacatc gatgtgacct gggaaacatt
      301 gtggaccctg aaggtgaaca agtggttcgg cgcgacactg aacaccttgc tgctgtacga
      361 ccatgacatc agcgtgccca aggtcaggac cgacgagagc accggcgcaa aagtgtccta
      421 catgggcaaa ggcacccagt tcaaggaggt gctcggcatc ggcctcacct tcaacctctg
      481 atcgcacccg atcatcgcac gtaacgagcc ccggcatgcc ggggctcgtt gcatttccgg
      541 cacagcccga tggatggtca tgcggccctc ctcactgcct tctggtggac caaagtgccg
      601 gcccgcatcc gcgccgaaat gccgtacgtg ggaaaatatt ccgccgtgat cgaaccggca
      661 cacctttcaa cggtcctacc cctgaaacaa cgcacccttg ggacggacaa ggccttcggt
      721 cagccggaca cggcttcgcc agcgataccc ggcgtccact gaccaaggtt gaaaacacat
      781 gaacctcctg ggcgaagcga tcggattttg ccgaccttcg tcacacaacg accggcatgg
      841 cgatcacccc ttacaccggc cccttcaccg ataccgagct gcggcacctc ctgcgccgca
      901 ccctgttcgg ttgcagcaac gccgatctcg accacttcgc aggtcaaacg ctgcagcagg
      961 tggtggacgc gttgctcacc ttcagcaacg ataccacacc gcccgtcaag acctattggc
     1021 tcctgaacgg caacacgccc gaccccactt tgatcgacgc gaacgtgccc ttcgggaaca
     1081 cgtggataaa tacgccgcgc cagacggcag acgagcccac cgtgagcggt aaacgcatcg
     1141 aaagcctcgt ggcgtggcgc accggcctgc tcgcacaaca ggaccggaca ctgcgcgaaa
     1201 aactcaccct tttctggaac aacctcgtgc ccaccgaggc gtccacg
//
LOCUS       DAOQOC010001096         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101284_length_1246_cov_2.214945, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001096 DAOQOC010000000
VERSION     DAOQOC010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101284_length_1246_cov_2.214945"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PL106_14705"
     CDS             <1..280
                     /locus_tag="PL106_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34375.1"
                     /translation="GQTVSRAKRQLVHDVWLSYRLIEPGLSQVATHARTHVIPIHLFF
                     GRYDRIIRPAFGGRLRDLAPEQVTMEELPFGHTLITPELARAMEERLQ"
     gene            complement(284..>1246)
                     /locus_tag="PL106_14710"
     CDS             complement(284..>1246)
                     /locus_tag="PL106_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34376.1"
                     /translation="FTAGLAWYTYGIDTVPGTNDQYLPGIPSGTDTRQEHTIDASGRM
                     GTTSFYYAANYKDRLYIGAALGITGTRYERYTTHEERPLDDAVALRTVHFKEDLLTTG
                     NGFDLKVGAIGRVGSHVRLGAAFHTPMWLLLNDSYSYTMTTTFTGSSDPLQEVSPDGT
                     FSYRVTTPWSATLSAVYQAGKYGLVSVDYTYSDPRNARLRASRSLLDQYDYGTENEAI
                     KASFRPMHTVRAGTEWRAGSWYFRVGGAWRPDPYAKADTRHGTGYRQYGGGVGYRVTH
                     WSFDLGGTWGLRNGKYYTYSPILVNPVEARYTDLRMLFTVAWRP"
BASE COUNT          225 a          414 c          374 g          233 t
ORIGIN      
        1 cgggcagacc gtcagccgcg ccaaacgcca gctcgtacat gacgtgtggc tgagttaccg
       61 actgatcgag cccggattgt cgcaggtggc cacccacgcc cgaacgcatg tcatccccat
      121 ccacctgttc ttcggcaggt acgaccggat cattcgaccg gcctttggtg gccgactgcg
      181 cgatctggca ccggaacagg tgacgatgga agagctgccc ttcggccaca cgctcatcac
      241 accggaactg gcgcgcgcga tggaagaacg attgcaatga ccctcacggc cgccacgcca
      301 ccgtgaacag catgcgcaga tcggtgtagc gcgcctccac agggttcacc aagatggggg
      361 aatacgtgta gtattttccg ttgcgcaggc cccacgtgcc gcccaggtcg aagctccagt
      421 gcgtgacgcg ataccccacg cccccgccgt actggcggta gccggtgcca tgacgcgtgt
      481 ccgccttcgc gtacggatcg ggccgccagg caccgcccac gcggaagtac cagctgcccg
      541 cgcgccactc cgtgcctgcc cgtaccgtgt gcatgggccg gaaactggcc ttgatcgcct
      601 cgttctccgt cccataatca tactggtcca ataaactccg gctggcccgc aggcgtgcgt
      661 tgcgcggatc gctgtacgtg taatccacgc tcaccagccc gtacttgccg gcctggtaca
      721 cggcgctcag cgttgcgctc caaggggtgg taacacggta gctgaaggtg ccgtccgggg
      781 atacctcctg taacggatcg ctactgcccg tgaacgtcgt ggtcatcgta tagctgtacg
      841 aatcgttcaa caacaaccac atgggcgtgt ggaacgccgc tcccaggcgc acatgcgagc
      901 ccacccgtcc gatggccccc accttcaggt cgaaaccgtt gcccgtggtg agcagatcct
      961 ccttgaaatg taccgttcgc agtgcaaccg catcgtcaag cggacgttcc tcatgcgtgg
     1021 tgtagcgttc gtaccgcgtg cccgtgatac ccagtgcggc gccgatgtac aaccggtcct
     1081 tgtagttggc cgcatagtag aagctggtgg tgcccatgcg gccggaggca tcgatcgtgt
     1141 gctcctggcg ggtatccgtg ccggatggga tcccgggcag atactgatcg ttggttccgg
     1201 gtaccgtatc gatcccgtac gtgtaccacg ccagaccggc cgtgaa
//
LOCUS       DAOQOC010001097         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101706_length_1243_cov_4.254209, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001097 DAOQOC010000000
VERSION     DAOQOC010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101706_length_1243_cov_4.254209"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PL106_14715"
     CDS             <1..570
                     /locus_tag="PL106_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNA34377.1"
                     /translation="LEDEIGGGDNLMVVKNNYFWAGENGKKELIANGEQILVKRMKGV
                     EEKFGLRFADLEVSWWNGSEDREMDVKVIVDVLAAEGPALPGPRMQLLAQSVMANILA
                     KNKAERFRLLKHDPYANALQVKYAYAVTCHKAQGGQWKSVFVDQGYVTEEMIDREYVR
                     WLYTAVTRASEKLYLLNFHPRFFGEGDPG"
     gene            complement(571..>1243)
                     /locus_tag="PL106_14720"
     CDS             complement(571..>1243)
                     /locus_tag="PL106_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNA34378.1"
                     /translation="VHIWYAKLDDIRPRHAEFDRLLQPEEQERAQRFRFDTDRERYVL
                     GHGLLRVLLGRYLTQEPASIRMARGRFGKPYVEGSDLHFSFSDTKDAILVAFATGAEI
                     GADIETMHRKVDHEGVSGHFFTGPEIGAITAAGPNAKRRFLEFWTRKEAVLKASGTGI
                     MDDLHSLRVDQDINELTIRHPEFVVMAAPVYHVHTWQVGADHIVSMASPQQRWEVKAW
                     NAIEV"
BASE COUNT          232 a          353 c          410 g          248 t
ORIGIN      
        1 ctggaggacg agataggagg gggcgataac ctgatggtgg tgaagaacaa ctacttctgg
       61 gcgggcgaga acgggaagaa ggaactgatc gccaacggtg agcagatcct ggtgaaacgc
      121 atgaaaggtg tggaggagaa attcggcctg cgcttcgctg atctggaggt gagctggtgg
      181 aacggctcgg aggatcgcga gatggacgtg aaggtgatcg tggatgtgct cgccgcagaa
      241 ggccctgcct tgcccggccc gcgcatgcag ctgctcgctc agagtgtgat ggcgaacatc
      301 ctcgccaaga acaaggccga acgctttcgg ctgttgaagc acgatccgta tgccaacgcc
      361 ctgcaggtga agtacgccta cgccgttacc tgccacaaag cgcagggcgg ccagtggaag
      421 agcgtcttcg tggatcaggg atacgtgact gaggagatga tcgatcggga gtatgtgcgc
      481 tggctgtaca cggcggtcac ccgggccagc gagaaattat acctgttgaa cttccatccg
      541 aggttcttcg gggaggggga tcctgggtga tcagacctcg atcgcgttcc atgccttcac
      601 ctcccacctt tgctgcgggc tggccatact cacaatgtgg tccgctccca cttgccaggt
      661 atgtacgtgg tacactggcg ctgccatgac cacgaattcc gggtgcctga tggtgagttc
      721 gttgatgtcc tggtccaccc gcaggctgtg cagatcgtcc atgatcccgg tgccgctggc
      781 cttcagtacg gcctccttgc gcgtccagaa ctccaggaag cgccgtttgg cgttgggccc
      841 ggcagcggtg atcgccccga tctcgggccc ggtgaagaag tgcccgctca caccttcgtg
      901 gtccaccttc cggtgcatcg tctcgatgtc cgcgccgatc tccgcaccgg tggcgaaggc
      961 gaccagtatc gcatccttcg tgtcgctgaa gctgaagtgc aggtcagagc cctccacata
     1021 cggtttgccg aagcggccgc gcgccatgcg gatggatgcg ggttcctggg tcagatacct
     1081 gccaagaagt acgcgcagca ggccgtggcc cagcacgtag cgctcgcggt cggtgtcgaa
     1141 acggaagcgc tgtgctcgtt cctgttcctc gggttggagc agcctgtcga attccgcatg
     1201 gcgcgggcgg atgtcgtcca gcttggcgta ccagatatgt acc
//
LOCUS       DAOQOC010001098         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101709_length_1243_cov_4.170034, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001098 DAOQOC010000000
VERSION     DAOQOC010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101709_length_1243_cov_4.170034"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1243
                     /locus_tag="PL106_14725"
     CDS             <1..>1243
                     /locus_tag="PL106_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNA34379.1"
                     /translation="PSPAWYAVQALPYLMEYPHECAEQTFSRYYANTLAAHIVNKRPR
                     IKAVFERWKHAGPDAFASALEKNTELKGVLLEETPWVLNARSERESKERIALLFDLQR
                     MGAEQATALKKLREMQLPNGAWPWWSGMRESRYITQHIVAGFGHLEKLDAVDLRPDGM
                     SQQMVKDAVRWLDGEVERAYRDLVKHTKKEELDRYVPGHSDIHFLYARSFFGRWTIDG
                     ATRTAVDFYLKRLKATWLQQGLQDQAMAALAMHRLGEPEVAQLIMRSLSERATRSEEL
                     GMFWKGFVSGFDWWSFPTETHALLIEAFNDVSGDTASVNALRTHLLRLKQTTDWKTTK
                     ATADACYALLLNGDNWLVDTGSPVITVGGTPISADQAEAGTGTFEQVFAPATVKPAMA
                     EVTITSNTVHPGWGALHWQYLE"
BASE COUNT          257 a          392 c          403 g          191 t
ORIGIN      
        1 cccagccccg cctggtacgc cgtgcaggca ctgccctacc tgatggagta cccgcacgaa
       61 tgcgccgagc agaccttcag ccggtattac gcgaacacgc tcgcggccca catcgtgaac
      121 aagcgcccac ggatcaaagc ggtcttcgag cgttggaagc atgcgggtcc cgacgcgttc
      181 gccagtgcgc tcgaaaagaa taccgaactc aaaggcgtgc tgctggagga gacaccctgg
      241 gtactgaacg cgcgcagcga acgggagagc aaggaacgca tcgccctgct gttcgacctg
      301 caacgcatgg gtgccgagca ggccaccgca ttgaagaagc tgcgcgaaat gcaactgccc
      361 aacggcgcct ggccgtggtg gagcggcatg cgcgagagcc gctacatcac tcagcacatc
      421 gtggccggat tcgggcactt ggagaagctg gacgcggtgg atcttcgacc cgacggtatg
      481 tcgcaacaga tggtgaagga tgctgtgcgt tggctggacg gcgaggtgga acgtgcgtac
      541 cgggatctgg tgaagcacac gaagaaggag gagctggacc ggtacgtgcc gggccattcg
      601 gacatccact tcctctacgc gcgcagcttc ttcggccgct ggacgatcga tggggccacc
      661 cgcaccgctg ttgacttcta cctgaagcgc ctgaaggcca cctggctgca acaaggactg
      721 caggaccagg ccatggcggc attggcgatg catcggctgg gtgaacccga agtggcccaa
      781 ctgatcatgc gttccctgag cgaacgggcc acccgcagcg aggagctcgg catgttctgg
      841 aaaggcttcg tcagcggctt cgactggtgg agcttcccca ccgagacgca tgcgctcctg
      901 atcgaggcct tcaacgacgt gtccggtgat acggccagcg tgaacgccct gcgcacacac
      961 ctgctccgcc tgaaacagac caccgactgg aagaccacca aggccacggc cgacgcatgc
     1021 tacgccttgc tcttgaacgg ggacaactgg ttggtggata ccggatcgcc ggtgatcacc
     1081 gtgggcggca cacccatcag tgccgaccag gccgaggccg gtacgggcac cttcgagcag
     1141 gtgttcgcgc cggcaacggt gaaacccgcc atggccgagg tgacgatcac gagcaacacg
     1201 gtccaccccg gctggggggc gctgcactgg cagtacctgg aac
//
LOCUS       DAOQOC010001099         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101744_length_1243_cov_3.069865, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001099 DAOQOC010000000
VERSION     DAOQOC010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101744_length_1243_cov_3.069865"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..960
                     /locus_tag="PL106_14730"
     CDS             121..960
                     /locus_tag="PL106_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34380.1"
                     /translation="MNFFLDDMRYLIHPGEWERNALNSAWDNSIEHRYDTRGGQGVVS
                     IKVRNSSIGSAHRFAQATLTASGSDRIGPLSLRTRLIAQFGTGDTPRESQLYLAGASP
                     EEMMENKYTRSVGFVPYDQGGYGSAITHFQAGGGLGLRGYAGYVAPESMDSTLGGQFL
                     VYRGNTGAAFNAELDLDGLVRLRPKATREWLHLDVYLFGDIGSMGYRRVTDGGSQQLR
                     FAYPRADAGAGLALTIKKFGPLDGIKPLTIRFDAPLFVSSLPNNEQDHFAFRYVIGIE
                     RAF"
     gene            965..>1243
                     /locus_tag="PL106_14735"
     CDS             965..>1243
                     /locus_tag="PL106_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34381.1"
                     /translation="MLNRGSSDQDVPYSGRWAVHRMLRHLLPAACFLLLPGTHAAQSI
                     FGRWVSVDDNTGKRRSIVEISERDGKAYGRIAYLFEEEKRTKKCNLCPA"
BASE COUNT          260 a          385 c          375 g          223 t
ORIGIN      
        1 aaaaaataaa ctcagtacag tttccgtgta tatatatccc aaaaatagat ctttgttcct
       61 atctttaaaa aaaaagcagt acaaaattat atttcatcta tcaatgataa aattaactcg
      121 atgaacttct tcttggacga catgcgctac ctgatccatc cgggcgaatg ggaacggaac
      181 gcgctgaaca gcgcgtggga caacagcatc gagcaccggt atgatacccg cggcggacag
      241 ggcgtggtat cgatcaaggt gcgcaacagt tccatcggtt cggcgcaccg cttcgcgcag
      301 gccacgctca cggcatccgg ctcggaccgc atcggcccgc tgagcctgcg cacacgcttg
      361 atcgcccaat tcggcacggg cgacaccccg cgcgagagcc agttgtacct ggccggggcc
      421 tcacccgagg agatgatgga gaacaagtac acgcgcagcg tcggtttcgt gccttacgac
      481 cagggcgggt atggcagtgc catcacgcac ttccaggcgg gcggtggcct gggcctgcga
      541 ggctacgcgg gctacgtggc ccccgaatcg atggacagca cgctcggagg ccagttcctc
      601 gtgtaccgcg gcaacacggg cgccgcattc aacgccgagc tcgacctgga cgggctggtg
      661 cggctgcggc ccaaggccac gcgcgagtgg ctccacttgg acgtgtatct tttcggcgac
      721 atcggcagca tgggttaccg gcgggtcacc gacgggggtt cccagcagct ccgcttcgcc
      781 tatccgcgag ccgatgccgg ggcgggcctg gccctgacca tcaagaaatt cggcccgttg
      841 gacggtatca aaccactcac catccgcttc gatgcgccgc tcttcgtgag cagcctgccg
      901 aacaacgagc aggaccactt cgcgttccgc tatgtcatcg gcatcgaacg tgcgttctga
      961 tcgcatgttg aacaggggat ccagcgatca ggatgtaccg tacagcgggc ggtgggcggt
     1021 acaccgcatg ctgcggcacc tgctgcccgc agcctgtttc ctgctcctgc cgggcaccca
     1081 tgccgcgcag agcattttcg ggcgctgggt atccgtggac gacaacaccg gcaagcggcg
     1141 cagcatcgtg gagatcagtg aacgcgacgg caaggcctac gggcggatcg cgtacctctt
     1201 cgaggaggag aagcgcacga agaaatgcaa cctctgcccg gcc
//
LOCUS       DAOQOC010001100         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_101878_length_1242_cov_3.316765, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001100 DAOQOC010000000
VERSION     DAOQOC010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101878_length_1242_cov_3.316765"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..>1242
                     /locus_tag="PL106_14740"
     CDS             75..>1242
                     /locus_tag="PL106_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34382.1"
                     /translation="MRRIHTLTLSLLLVTGLQAQIQIGQNEMPHAGDELHRTRATPDQ
                     GIDYASTGPAHEWNFVDLSAANEEVKDYVTVGSTNFVYSLAFADIFFNPNRANHAVSG
                     TDIPFNQLLPIENPWTFLYHSATVYRKVGYGVELSGIPVPIIFNSPDVIYELPLNFGN
                     TSSSSSGYELNLPSLAYYGYSQERSNEVDGWGTIATPAGTFEALRVKSTIAGHDTIAV
                     DTLGVGFNIDRPLVHEYKWLAQGIRVPVLQINTTEIFGNEVVTGIWYHDQPHTIFVED
                     PLASTLCPGSTFTLYYGITGSYNEGGFFIPANHFTAELSDANGDFSAPTTIGDVTANL
                     AGGITVTIPPNTPPGTGYRIRVNSTQPAYTGSDNGFDITIDGAAPSAVISADGPL"
BASE COUNT          242 a          442 c          340 g          218 t
ORIGIN      
        1 gtgatgaaga cccgccggcg cattgactac ggcgcccgca gcgtggaact tcgtgccgtc
       61 aaccataccc gaacatgcgc cggatccata ccctaacact ctccctcctt ctcgttaccg
      121 gcctccaggc ccagatccag atcggccaga acgagatgcc ccatgcgggt gatgaactgc
      181 atcgcacccg cgccacgccc gaccagggca tcgactacgc cagcacaggc cccgcgcacg
      241 agtggaactt cgtggacctg tccgccgcca acgaggaggt gaaggactac gtcaccgtcg
      301 gctccaccaa cttcgtgtac tcgctggcct tcgccgacat cttcttcaac cccaaccggg
      361 ccaaccacgc ggtatccggc acggacatcc cgttcaacca gctgctgccc atcgagaacc
      421 cgtggacctt cctgtaccac agcgccaccg tgtaccggaa agtgggttac ggtgtggaac
      481 tttccggcat cccggtgccc atcatcttca actcgccgga tgtgatctac gagttgccgc
      541 tgaacttcgg gaacacgagc tcctccagct cgggttacga gttgaacctc ccttcgttgg
      601 cgtattatgg ctacagccag gaacggagca acgaggtgga cggatggggc accatcgcca
      661 ccccggcagg caccttcgag gccctgcgcg tgaagtccac catcgccggg cacgatacca
      721 tcgccgtgga caccttgggg gtgggattca acatcgaccg tccgttggtg catgagtaca
      781 aatggctggc ccagggcatc cgcgtgcccg tgctgcagat caacaccacc gagatcttcg
      841 gcaacgaggt cgtcacgggg atctggtacc acgaccagcc gcacaccatt ttcgtggaag
      901 acccgctggc gagcacgctc tgccccggct ccacgttcac cttgtattac gggatcaccg
      961 ggagttataa tgagggcggg ttcttcatcc cggccaacca tttcaccgct gagctctccg
     1021 acgcgaacgg cgatttcagc gctccgacga cgatcggcga tgtgaccgcc aacctcgctg
     1081 gcggcatcac ggtgaccatt ccgcccaaca cgccaccggg caccggctac cgcatccgcg
     1141 tgaactcgac ccagccggcc tatacgggct cggacaacgg cttcgacatc accatcgacg
     1201 gcgctgcccc ctcggccgtg atctccgctg atggcccctt gg
//
LOCUS       DAOQOC010001101         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_102088_length_1241_cov_2.827993, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001101 DAOQOC010000000
VERSION     DAOQOC010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102088_length_1241_cov_2.827993"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PL106_14745"
     CDS             <1..213
                     /locus_tag="PL106_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34383.1"
                     /translation="VGVKKFQYDIWGDTVNTASRMESSGEVGQVNISEATYALVKDEG
                     SFTFTPRGKVQAKGKGEMEMYFVHQT"
     gene            210..>1241
                     /locus_tag="PL106_14750"
     CDS             210..>1241
                     /locus_tag="PL106_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34384.1"
                     /translation="MKRVDLLLLACLLYLTTAHAQLARSVSLEKELARHRKEDAAKAY
                     LMAELAKTVSTIDPDRAFALSEQAVALAQRLKDDKALAAAWVSQRWALYYQGFYQRCD
                     SVNKLAAAAAKRTGDARSWLLAYGAPHYISGSKDSAVCAKMAMRMRRALQIADSLGEG
                     ELAGEVLLWHGMKPWGRPNARALEEARVRYRSAGDSTGVGMCLFCIRSFEPYSTDRAL
                     NQRCIEQATALFDLDGNTLWSLWGHSELSGMATEQADYKTAVQEGLEAFRRAEQLGME
                     REMLGASNALASAYRDIGDVRAALDLDLRGLELVSELDEWLAVRNQLGRTYRLFGMPD
                     SAAFHLRSSL"
BASE COUNT          255 a          349 c          401 g          236 t
ORIGIN      
        1 gtgggcgtga agaaattcca gtacgatatc tggggcgata cggtgaacac ggcatcacgg
       61 atggaatcct caggcgaagt gggacaggtg aatatcagcg aggcgacgta cgcgctggtg
      121 aaggatgaag gctccttcac cttcacgccg cgcggaaaag tgcaggcaaa ggggaaggga
      181 gaaatggaga tgtactttgt tcaccagaca tgaaaagggt cgatctgctt ctgttggcgt
      241 gcctgctgta cctgaccact gcacacgctc aactggcgcg ttcggtcagc ctggagaaag
      301 agttggccag gcaccgcaag gaagatgcgg cgaaagcgta cttaatggcg gagcttgcaa
      361 agacggtttc aacgatcgat cctgatcggg cctttgcact ttcagagcaa gccgtggcgc
      421 tcgcacagcg attgaaggat gacaaggccc ttgccgcggc ctgggtgagc caacgctggg
      481 cgctctacta ccaaggcttt tatcagcgat gcgattccgt gaacaaactg gctgccgccg
      541 ccgcgaagcg cactggcgac gcccgctcct ggttgctcgc ctacggcgcc ccgcactaca
      601 tcagcggctc gaaggacagc gccgtgtgcg cgaagatggc catgcgcatg cgtcgtgccc
      661 tgcagatcgc cgactcgctg ggggaaggcg aactggccgg cgaagtgctc ttgtggcacg
      721 gcatgaaacc ttgggggaga ccgaatgccc gtgctctcga ggaagccagg gtgcgctacc
      781 gctccgccgg cgactcaacc ggcgtgggca tgtgcctgtt ctgcatccgc tccttcgaac
      841 cctactccac ggatagggcg ctgaaccagc gctgcatcga gcaggcaacc gcattattcg
      901 accttgatgg caacacctta tggtctcttt gggggcattc tgagctgagt ggcatggcga
      961 ctgaacaggc cgattacaag accgccgttc aggaagggct tgaagcgttc cggcgggctg
     1021 aacaactagg tatggaacgc gagatgctcg gcgcaagcaa tgccttggcc tccgcatacc
     1081 gggatatagg tgatgtgcgt gccgccctcg acctggatct gcgcggcctg gaactcgtat
     1141 cggagctcga tgagtggttg gcggtgcgga accagctggg ccggacatac cgtttgttcg
     1201 gcatgccgga tagtgccgcc ttccacttgc gatcctccct g
//
LOCUS       DAOQOC010001102         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_102540_length_1239_cov_1.448480, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001102 DAOQOC010000000
VERSION     DAOQOC010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102540_length_1239_cov_1.448480"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PL106_14755"
     CDS             <1..598
                     /locus_tag="PL106_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34385.1"
                     /translation="PTAGTGSLLFLDQNDPNTDMREAYKLVAWVGCPMLYPGYSFKDP
                     KEIPTNATIKLRVNQPIRSRGGTTDYPIFTWSTDDLAAEAGVTAVAQNSLLNNVRVVP
                     NPYYGFSTYERSQLQTIVQITNLPRKCTIKIYNLSGTLIRTYMKDSDNPSQNWDLKNA
                     SGTPVASGPYIIHVDGGDLGETIVKFMAIMPELDLNAF"
     gene            618..>1239
                     /locus_tag="PL106_14760"
     CDS             618..>1239
                     /locus_tag="PL106_14760"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNA34386.1"
                     /translation="MKKILILGLALCAAVGQTFAGNDQRAGQAGATQLLVNTWGRSSG
                     YNGINIGSASGIESIITNPAGLAMTRRTELVFAHTRYLVGSDISVNSFGISQALKRAG
                     VIGMYVTAFDLGTFIRTTEANPEGELGTFRPTFMNLGLTYGKKFTDHISVGATIKLVT
                     ESINNANASGIAFDAGVQYRTNLGKDSLHNDKLKIGITLRNVGSTMR"
BASE COUNT          288 a          385 c          320 g          246 t
ORIGIN      
        1 gcctaccgca ggaactggtt cgctcctctt cctcgatcaa aacgacccca acaccgacat
       61 gcgcgaggct tacaagctgg tcgcatgggt gggttgcccg atgctctacc ccggctactc
      121 cttcaaggat cccaaggaaa tccccaccaa cgcgaccatc aagttgcgtg tgaaccagcc
      181 catccgttcg cggggaggca ccacggatta cccgatcttt acttggagca ccgacgacct
      241 cgctgctgaa gctggcgtga ccgcagtggc tcagaactcc ctgctgaaca atgtccgtgt
      301 tgtgccgaac ccctactacg gcttcagcac ctacgagcgt tcgcagttgc agaccatcgt
      361 tcagatcacc aacctgcccc gcaagtgtac catcaagatc tacaacttga gcggtaccct
      421 gatcaggacc tacatgaagg attccgacaa ccccagccaa aactgggatt tgaagaacgc
      481 cagcggtacg cctgttgctt cgggtccgta catcatccac gtggacggtg gcgatctggg
      541 tgaaaccatc gtgaaattca tggctatcat gccggaactg gatttgaatg cgttctaaca
      601 ttcaacggat aagagagatg aagaaaattc taattcttgg attggcactc tgcgccgctg
      661 tgggacaaac cttcgctggc aatgaccagc gcgcaggcca ggccggagcc acccagctct
      721 tggtgaatac ttggggccgc tccagtggct acaacggtat caacatcggg agtgcctccg
      781 gtatcgagag cattatcacc aaccccgctg gcttggcaat gacacgccgg acggaactgg
      841 tgtttgctca cacccgttac ctggtgggct cagacatctc cgtgaacagc ttcggtatct
      901 cccaagccct caagcgggcc ggtgtgatcg gtatgtatgt cacggcgttt gacctgggca
      961 ccttcatccg tacgaccgag gccaaccctg aaggggaact gggcacattc cgcccgacct
     1021 tcatgaacct cggcctcacc tacggcaaga agttcaccga ccacatctct gtgggcgcga
     1081 ccatcaagct ggtgaccgaa agcatcaaca acgcgaatgc aagcggcatc gccttcgacg
     1141 caggtgtgca ataccgcacc aacctgggca aggactccct gcacaatgac aagttgaaga
     1201 tcggtatcac cctgcgcaac gtgggttcca ccatgcgct
//
LOCUS       DAOQOC010001103         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_103176_length_1235_cov_2.242373, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001103 DAOQOC010000000
VERSION     DAOQOC010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103176_length_1235_cov_2.242373"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PL106_14765"
     CDS             <1..448
                     /locus_tag="PL106_14765"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNA34387.1"
                     /translation="NRTNTLLLAAGLFIVLTSEKCNKTPEAAAGGTPAAAASIFGSRW
                     NLATLAGAAVRMPEGKENPYLSIAEDLSVSGFGGCNRLVGQAKVEGSAVSFPGLGSTK
                     MYCHDTQETESSFLKALNATNTFKLDGDKLVLLDKATELATLIKGK"
     gene            551..901
                     /locus_tag="PL106_14770"
     CDS             551..901
                     /locus_tag="PL106_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34388.1"
                     /translation="MEHRRQRLATWATYLRRQLYFLLAAVVLIGLSLTIGTLGYMFFA
                     NYGAADAFLNASMILSGMGPLGALPGDDAKWFASAYALFSGIALPTIVAVMLAPFVHR
                     ILHKLHLDDADRND"
     gene            complement(1008..>1235)
                     /locus_tag="PL106_14775"
     CDS             complement(1008..>1235)
                     /locus_tag="PL106_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34389.1"
                     /translation="PFNENTVIRFYVPPTAKSATIDVNAADGSTWRSFNALTGNGQLV
                     IGAGSMSEGTYTYSLVVDGERIDTRTMVITR"
BASE COUNT          229 a          398 c          367 g          241 t
ORIGIN      
        1 gaaccgtacg aacaccctcc tgctggccgc tggcctcttc atcgtgctca cctcggagaa
       61 atgcaacaag acccctgaag cggctgccgg tgggactcct gcagcggcag cctccatctt
      121 cggaagtcgc tggaacttgg ccacgctggc cggagcggcc gttcgtatgc ccgagggcaa
      181 ggagaacccc tacctgtcca tcgccgagga cctgagcgtg tccggctttg gcggttgcaa
      241 caggctggtg ggccaggcga aggtggaagg cagcgccgta tcgttccccg gcctgggtag
      301 caccaaaatg tactgccacg acacccagga gacggagtcc tcattcctga aggccctgaa
      361 cgccaccaac accttcaagc tcgatggcga caagctggtg ctgctggaca aggccacgga
      421 gttggccacg ctgatcaagg ggaagtgatc gtgcctgaac atcggcaaat aatttcaccg
      481 ccccttcagg atccggcccc tccatatcgg tggggatccg agcaaataga gagttgagta
      541 tcacgatcgg atggagcacc gtcgccaacg cttggccact tgggccacct acctgcggcg
      601 gcagttgtac tttctgctcg ctgcggtcgt gctgatcggc ctcagcctca ccatcgggac
      661 cctgggatac atgttcttcg ccaactacgg cgcagccgat gcattcctga acgcgagcat
      721 gatcctcagc ggcatgggcc ctttgggcgc tctgcccggc gacgatgcca aatggttcgc
      781 ttcggcctac gccctgttca gtggcatcgc gctgcccacc atcgttgcgg tgatgctggc
      841 ccctttcgtt caccggatcc tgcacaagct gcatctggac gacgcggacc ggaacgactg
      901 atacaactcg gggccttccg ggacgcccgg gtgttcaaat gccgggtccc gagcacggac
      961 aggggccgcc ttggtgaaag gcgacccctg ttcttcgaat gtcctgctca gcgcgtgatc
     1021 accatcgtgc gcgtgtcgat acgctcacca tcgaccacga gactgtacgt gtatgtgcct
     1081 tcgctcatgc taccagcccc gatcaccaac tggccgttcc ccgtcaatgc gttgaacgat
     1141 ctccaggtac tgccgtccgc ggcgttcaca tcgatcgtgg ccgacttggc cgtgggcggc
     1201 acgtagaagc ggatcacggt gttctcgttg aaggg
//
LOCUS       DAOQOC010001104         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_103474_length_1233_cov_3.156197, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001104 DAOQOC010000000
VERSION     DAOQOC010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103474_length_1233_cov_3.156197"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PL106_14780"
     CDS             <1..725
                     /locus_tag="PL106_14780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HNA34390.1"
                     /translation="IGDCDGYVNVVDGEGVLFSIDQGFTWSNTNVLTGLCAGPVTVLM
                     QDANGCLASATTTIASPPPVTAGFTPTPDTMNVTATTVAFLNQSGNATQFVWDFGGLG
                     TSTEGDPAFTFPDALGGVYTVCLTAMDANGCLDSVCLPIVVLDELQVSVPNAFTPNGD
                     DINEVFVPVLNVAASAEAYTFMIFDRWGLLLFSTEQAGQGWDGTYGGQPVPNDVYVWK
                     VRCHDRVTGKDRDLIGHVTVIR"
     gene            734..1138
                     /locus_tag="PL106_14785"
     CDS             734..1138
                     /locus_tag="PL106_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34391.1"
                     /translation="MLPSRSFVFALSCSPLLAFAQGVQLPQGPPYGCACGFVGVDPEP
                     RTTIEALILRRDTAGIFSYLRDTSAVHQAYGAEAVIRLSRAGMAIPKHVLVIADDLRK
                     SDRMVYVCSGCIYWMEEMRVALRDSMPVNNIR"
BASE COUNT          228 a          384 c          375 g          246 t
ORIGIN      
        1 gtatcggcga ttgcgacggc tatgtgaacg tggtcgatgg cgaaggcgtg ctcttcagca
       61 tcgatcaggg cttcacatgg tccaacacca acgtactgac cgggctgtgc gccggtccgg
      121 ttaccgtgct gatgcaggat gccaacggct gtttggcgag tgccactacc accatcgcga
      181 gcccaccgcc cgtaaccgca ggattcacgc caaccccgga caccatgaac gtgaccgcaa
      241 ccaccgtggc cttcctcaac cagagcggca acgccaccca attcgtttgg gacttcggcg
      301 gcctgggcac cagcaccgaa ggtgaccccg ctttcacctt ccctgatgcg ctcggcggtg
      361 tttacacggt gtgcctcacg gccatggatg ccaacggctg tctggacagc gtttgcctcc
      421 cgatcgtggt cctggacgaa ctgcaggtct ccgtgccgaa cgcgttcacc ccgaacggcg
      481 acgatatcaa cgaggtcttc gtgcccgtgc tcaacgtggc cgcgagcgct gaggcctaca
      541 cattcatgat ctttgaccgc tggggcctgc tgctcttcag caccgaacaa gccgggcagg
      601 gctgggatgg gacctatggc ggtcaacccg taccgaacga tgtgtacgtt tggaaggtgc
      661 gctgccacga ccgcgtgacg gggaaagacc gggatctcat cgggcatgtg acggtgatcc
      721 ggtagcttcg tgcgtgctcc cctcccgttc attcgtcttc gctctctcgt gttcgccctt
      781 gctggccttc gcgcagggcg ttcagttgcc ccaggggcca ccatatggct gtgcctgtgg
      841 ttttgtcggg gtggatcccg aaccgcgcac aacgatcgaa gcgctgatcc tccggaggga
      901 taccgcaggc atcttcagct atttgcgcga cacctccgcc gtgcatcaag catatggtgc
      961 cgaagcggtg atccgactga gcagggcagg catggccatc ccaaagcacg tgttggtgat
     1021 cgctgacgac ctccggaaat ccgatcgcat ggtgtacgtg tgcagcggtt gcatctactg
     1081 gatggaagaa atgcgggtcg cccttcgcga cagtatgccg gtgaacaaca tcaggtagcc
     1141 gatcgaggtg ctcggaaatg tgtgcgccca gcatgccacg catggatcac atcacaccct
     1201 gcgttggtag cgaagcgggc ccctgcgagg taa
//
LOCUS       DAOQOC010001105         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_103640_length_1232_cov_3.496177, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001105 DAOQOC010000000
VERSION     DAOQOC010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103640_length_1232_cov_3.496177"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PL106_14790"
     CDS             complement(<1..993)
                     /locus_tag="PL106_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34392.1"
                     /translation="MQALNTPSQHLKRNAVLVLLLGLWGSCTAQGTWDIIAPGPVLAD
                     ACGECQRVLQAKPKEVQLGLYTDENNDIWFVITDAAWLDKLFTGPNDGLAVDVIPSSY
                     YDCAEGPPANTSFHKGALLKPVYRSEIRAQTHVTPTGSAMVKAGHLPAAIVNGGPFEL
                     SLILLKDRNVCHTNSFFDLDSYRWDLLNMGLYMDTLSYGEQFDTTRHHSAAAIIKRKA
                     LHFTIPFERGKSTYSPADLRPLYDSLRLTDFTITRITIEAYSSVEGPEDRNIALQEQR
                     AQSIVDALQSYQDPSIERSVKASENWVEFLRDVRLTSYAELALMDRAQVKARLRD"
BASE COUNT          219 a          327 c          407 g          279 t
ORIGIN      
        1 gtcgcggagc cgtgctttca cctgcgcgcg gtccatcagg gcgagttcgg catacgaggt
       61 gagtcgcaca tcgcgcagga attccaccca gttctcactg gccttcacgc ttcgttcgat
      121 gctaggatcc tggtagctct gcaacgcgtc cacgatgctt tgtgcacgct gctcctgcaa
      181 ggcgatgttg cggtcctcgg gcccctcaac gctgctgtac gcttctatgg tgatgcgggt
      241 gatggtgaag tccgtgagcc gcaatgaatc gtacagtgga cgcaggtcgg cgggtgagta
      301 tgtgcttttg ccgcgttcga agggaatggt gaaatgcagc gccttgcgtt tgatgatggc
      361 agcggcgctg tggtgtcgtg tggtatcgaa ttgctccccg tagctgagcg tgtccatgta
      421 caggcccatg ttcaggaggt cccagcggta gctgtccagg tcgaagaagc tgttcgtgtg
      481 gcaaacgttc cggtccttca gcaggatgag cgagagttcg aacggcccgc cattcacgat
      541 cgctgcgggc agatgcccgg ctttcaccat ggccgatccg gtgggggtta cgtgcgtttg
      601 ggcgcggatc tccgagcggt acacgggctt gagcagggcg cccttgtgga agctggtgtt
      661 ggcgggaggg ccttcggcgc agtcgtagta cgaactgggt atcacatcca cggcaagtcc
      721 gtcgttcggt cccgtgaaga gtttgtccag ccaagcggca tcggtgatca cgaaccagat
      781 atcgttgttc tcatcggtat aaaggccgag ctgaacctct ttgggcttgg cctgcaatac
      841 gcgctggcat tcgccgcatg catcggccag caccggtccg ggcgcgatga tgtcccatgt
      901 gccttgtgcc gtgcacgatc cccagagccc aaggagcagg acgagtacgg cgttcctttt
      961 caggtgttgg gaaggcgtgt tcagcgcttg catcgttcat ggaatgcgga agaggtacat
     1021 gacggagcgc aagaagagaa gggaccacta cggtctactc agacggagat gaagaagcgg
     1081 tgtcgaggtg gtccttgtgt tgaagggtga atgagtgcaa gagtgcgagc gacgtggttt
     1141 tcgcgctcgc accctttctc tcgacctccg tcgtaattcc tattgaatgt cacggtgagc
     1201 ccgtcgaccg cgcctttgcg tagccgctac gg
//
LOCUS       DAOQOC010001106         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_103663_length_1232_cov_2.936279, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001106 DAOQOC010000000
VERSION     DAOQOC010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103663_length_1232_cov_2.936279"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PL106_14795"
     CDS             <1..757
                     /locus_tag="PL106_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNA34393.1"
                     /translation="RSFADLCTGPVLVHERVKHHFQGRPNVSTYSLGPESPARAENIR
                     VEDGAYHFDLVSDGSELRTLPLGMPGRHNVENAIAASTLALRAGVPTSRLREALASFK
                     GVSRRFELRAKTNGSVFIDDYAHHPAELEACIRSAKELYPGRKVTGIFQPHLFTRTRD
                     LAAGFAKALALLDELILLEIYPAREQPIPGITSHWLLDQVPMMNKAVCTKQELPSVLR
                     ARNIEILLALGAGDIDRLVPGIAALIEAIAKAR"
     gene            754..>1232
                     /locus_tag="PL106_14800"
     CDS             754..>1232
                     /locus_tag="PL106_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34394.1"
                     /translation="MKEKLQRLWRPARIILGAVTAVALLSFSAHNTNTTPVIELDVEL
                     VAPDGACFIDENAVREQVLNSTDAVIGAPIGMVDLAAIEAGLRRIGCVAHADVFHTLD
                     GTLHVKVRQREPIVRVINADGSGFYIDSEGWTMPLSDAHSARVLVVTGAVTEPFAALP
                     "
BASE COUNT          229 a          422 c          383 g          198 t
ORIGIN      
        1 ccgctccttc gcggacctgt gcaccggccc cgtactggta cacgaacggg tgaagcacca
       61 cttccagggc aggcccaacg tgagcaccta ttccttgggg cctgagagcc cggcgcgggc
      121 ggagaacatc cgggtggagg acggggccta tcacttcgac ctggtctccg acggatcgga
      181 actgcgcacc ctccccttgg gcatgcccgg ccgccacaac gtggagaacg ccatcgccgc
      241 cagcacgctc gcgttgcgtg caggggtgcc cacaagccgc ttgcgcgagg ccctggcctc
      301 cttcaagggg gtcagccgac gatttgaatt gcgcgcgaag accaacggat ccgtgttcat
      361 cgatgactat gcccatcacc cggccgaact ggaagcctgc atccgcagtg cgaaggagct
      421 ttatcccggg aggaaggtca cgggcatctt ccagccgcac ctcttcacgc gtacacgcga
      481 cctggcggct ggattcgcga aggccctggc cctgctggac gagctgatcc tcctggagat
      541 ctaccccgcc cgcgaacaac cgatccccgg catcaccagc cactggctcc tggatcaggt
      601 gcccatgatg aacaaggccg tgtgtacaaa gcaggagctg ccctccgtcc tccgtgcgcg
      661 gaacattgaa atcttgctcg cgcttggcgc gggcgatatc gaccgcctcg ttccgggcat
      721 cgccgcactg atcgaagcga tcgcaaaagc ccgatgaaag agaagctcca acgcctgtgg
      781 cggcccgccc ggatcatcct gggcgcggtg accgccgtgg cattgctctc cttcagtgcg
      841 cacaacacga acaccacgcc ggtaatagaa ctggacgtgg aactggtcgc acccgacggt
      901 gcatgcttca tcgacgagaa cgcggtgcgc gaacaggtat tgaattcgac cgacgccgtg
      961 atcggcgcac ccatcgggat ggtggacctg gccgccatcg aggccggact gcgccggatc
     1021 gggtgcgtgg cccatgccga cgtgttccac accctggacg gcacgctgca cgtgaaggtg
     1081 cgccagcgcg agcccatcgt ccgggtgatc aacgccgacg gcagcgggtt ctacatcgac
     1141 agcgaaggct ggaccatgcc gttgagcgat gcgcacagcg cccgcgtact cgtggtgacc
     1201 ggtgccgtga ccgagccctt cgccgcactc cc
//
LOCUS       DAOQOC010001107         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_103760_length_1232_cov_1.885302, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001107 DAOQOC010000000
VERSION     DAOQOC010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103760_length_1232_cov_1.885302"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PL106_14805"
     CDS             <1..482
                     /locus_tag="PL106_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34395.1"
                     /translation="SSPAGKAVPPGLGTSLTVTPATFSGRTNYQWRLTTTNVAGANYN
                     NSGTNRTLTITSMQATNAGPYVVVVNDGFNALSLTSSPAATLTLALNQIIGSTLSGST
                     LNLAFPSEVGPQYVVEYKNALTNGVWIGLSTNTGTGSPIVVPVNLADPEKYFRLRMQ"
     gene            554..>1232
                     /locus_tag="PL106_14810"
     CDS             554..>1232
                     /locus_tag="PL106_14810"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNA34396.1"
                     /translation="MKCTSSQYRHSRDRHFRFGGFTLIELLVVIAIIAILAAMLLPAL
                     GRAKFKAHGISCMSNTKQITMAWMMWSGDNNDELLGARAWMGGDVGNPSSLDFVDANG
                     DLAKSPLNSYVGGNIKIYKCPGDPRVSKIALGPKWPAGTPASRSVCMNNYIAATDTWT
                     PDYLYFQKSSHLTRPGPVNTFVITDEGPTINDAYFATDMDTYDPNNMPGKRTTDAPAS
                     YHNKAGSF"
BASE COUNT          269 a          384 c          334 g          245 t
ORIGIN      
        1 tcagttcgcc agccggcaag gccgtgccgc cggggcttgg cacgtcactt accgttacgc
       61 cggccacctt ctccggtcgg accaattatc aatggcggct cacgaccacc aacgtcgcgg
      121 gcgccaacta caacaactcc ggcacgaatc gaacgctcac catcacgagt atgcaggcca
      181 cgaacgccgg cccgtatgtc gttgtggtca acgacggctt caacgcgctg tcgttgacga
      241 gttcaccggc ggcaacgctc acgctggcgc tcaaccagat cattggcagt acgctgagcg
      301 gctcgacgtt gaacctggcc ttcccctcgg aagtcggccc tcaatacgtc gttgagtata
      361 agaacgcatt gaccaatgga gtgtggatcg ggctcagcac caacacgggt accggctcgc
      421 ccatcgtcgt tccggtaaat ctcgctgatc ccgagaagta cttccgtctc cgcatgcaat
      481 agtggttgga gttggttagg ggaggcatcc gctcggacgg atgcctcctc gtcatgtcaa
      541 ccaacggcga tctatgaaat gcacgagttc ccagtaccgc cattcgcgag atcggcactt
      601 tcgcttcggt ggcttcacgc tgatcgaatt gctcgtcgtc atcgccatca tcgccatcct
      661 ggcggccatg ctgctgcctg ccctggggcg ggcgaagttc aaggcccacg gcatcagttg
      721 catgagcaac accaagcaga tcaccatggc ttggatgatg tggagcgggg ataataacga
      781 cgaactcctc ggtgcacgcg catggatggg aggagacgtg ggtaatcctt ccagcttgga
      841 tttcgtggat gcaaatggtg acctggccaa gagtccattg aattcctacg tgggtggcaa
      901 cattaagatc tacaagtgtc ctggcgaccc gcgtgtgagc aaaatcgcac tcgggccaaa
      961 gtggcctgct ggcactccag cttcccgaag cgtttgcatg aacaattaca tcgcggcaac
     1021 cgacacttgg acccctgatt atttgtactt ccagaagtcc agccacctca cccggccggg
     1081 ccccgtcaat acctttgtca tcaccgacga aggtccgacg atcaacgacg cctatttcgc
     1141 caccgacatg gacacgtacg atccgaataa catgcccggc aagcgaacca ccgatgcgcc
     1201 ggccagctat cacaacaagg caggcagctt ct
//
LOCUS       DAOQOC010001108         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104178_length_1229_cov_4.049404, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001108 DAOQOC010000000
VERSION     DAOQOC010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104178_length_1229_cov_4.049404"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PL106_14815"
     CDS             complement(<1..458)
                     /locus_tag="PL106_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HNA34397.1"
                     /translation="MRIPPISRHLAVLGFIGTLVPVASGQCDRWQQRIRCELTTDLDV
                     RTHRFTGTEKLSYTNNSPDTLRQLFFHLYFNAFKPGSEMDERSRAIVDPDPRVGDRIA
                     LLGPQEVGDLHCTNMLQDGKAVGSEELGTILRVKLARPVLPGKSTTLTFN"
     gene            complement(547..1020)
                     /locus_tag="PL106_14820"
     CDS             complement(547..1020)
                     /locus_tag="PL106_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA34398.1"
                     /translation="MFTHQPLPASVLHVDDSAKERAAFMAAFGSAFDLRSAPDANGAI
                     GILDRGGIHVLVVAQDLPEMKGAAFLGLVRQKFPQVRCMLTMSRPDPQLIVQALNHGG
                     VRHIINQPWDVAEVRKAVAAAVADHQAELDRTAFTARLIESNRQLEFALRQHLLS"
BASE COUNT          249 a          349 c          384 g          247 t
ORIGIN      
        1 aaattgaagg tgagcgtcgt gctcttcccg ggaagtacgg gccgggccag tttcacccgc
       61 aggatcgttc cgagctcctc cgaccccacc gctttgccgt cctgcagcat gttcgtgcaa
      121 tgcagatcgc ccacttcctg cggtccgagc agggcgatgc ggtcccctac ccgcgggtcg
      181 ggatccacga tggcgcgtga gcgctcatcc atctcactgc ccggcttgaa cgcgttgaag
      241 tagaggtgga agaacagctg acggagggta tccgggctgt tgttcgtgta gctgagcttc
      301 tcggtaccgg taaagcggtg cgtgcgcaca tccaggtcgg tggtgagttc gcagcggatg
      361 cgttgctgcc agcgatcgca ctggccggat gccacaggga caagggttcc gatgaagccg
      421 agaacggcaa ggtgcctgga aatgggagga atgcgcatgg gcggcgaata tcccgctctc
      481 cgcacgacct tttgcaaaga aggagacggg aagttgcgtc ctggatgctc cgggctgaat
      541 gtcgggtcag gacaacaggt gctgacgcag ggcgaactcc agttggcggt tgctctcgat
      601 cagtctcgcg gtaaaggccg tgcggtccaa ttcggcctga tgatcggcca ctgcggcagc
      661 cacagcctta cgcacctcgg ccacgtccca aggctggttg atgatgtgac ggaccccacc
      721 gtgattgagc gcttgtacga tcaactgcgg atcgggacgg gacatggtca gcatgcaacg
      781 gacctgtggg aacttctggc gcaccagacc aaggaaggcc gcgcccttca tctcgggcaa
      841 gtcctgtgca accacgagta catggatacc tccgcggtcc agtatcccga tcgccccgtt
      901 ggcatcaggg gccgatcgca gatcgaacgc ggagccgaac gcggccatga aagctgcacg
      961 ttccttggcc gaatcatcca catgaagtac cgaagcgggg agcggctgat gagtgaacat
     1021 ggtggctgga acgcgaactg ttagtatggg gttccgcaga aggaaaaaat gtgaaaaact
     1081 tgttcatatg ttgttcataa ctttttcgct gttagattcg cccccgctcc tcagtttgtt
     1141 catcgaaaaa ctcccctcga tccatgcttg gacttgatga acgtgcgtgc gttaccgcag
     1201 accggcggat gctccaccgc ccggcattt
//
LOCUS       DAOQOC010001109         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104188_length_1229_cov_3.532368, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001109 DAOQOC010000000
VERSION     DAOQOC010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104188_length_1229_cov_3.532368"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PL106_14825"
     CDS             complement(<1..468)
                     /locus_tag="PL106_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34399.1"
                     /translation="MTSTHLLIGIVSLAMLSWLRARREPGSAGPWIGVMLGLALATEL
                     SAAVLRNLLHAPNTSLYNVYVGLEFILFMRIATLSAPVGRAWTIMSMVVGLGALAGSW
                     WVHRDETFLLTEGILIVSTITAVWSIRVLWYLAQQEDRPLWQRPLFWFFTGSMV"
     gene            complement(486..>1229)
                     /locus_tag="PL106_14830"
     CDS             complement(486..>1229)
                     /locus_tag="PL106_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34400.1"
                     /translation="TAKELSCVEGEFGTHVHWGTSSTHVPLDTQVIRFTVESYVALRD
                     ARTCKGDSLLGLMVHYGADTSAKAKRVSLAFSMVCLAQPDTIGQSGYRIHEPLYAIDA
                     NAKLAMANTTMGAWRSWMGKRFQDSVVVDTKDDSVFDQRYQGLDAHMIFRCSDIDTLI
                     AHNQLAPTDHLELVPIAEPVSWDAHNRGEDFSMHLCLVAQMAGQRLISDSANTKTFEK
                     RGSDLGSACPPTCVKAAFPDSGVAVRPGC"
BASE COUNT          255 a          371 c          367 g          236 t
ORIGIN      
        1 gaccatggag ccggtaaaga accagaacag cggacgttgc cacagcgggc gatcctcctg
       61 ctgtgccagg taccatagca cgcggatcga ccatacagca gtgatcgtgg aaacgatcag
      121 gatgccctcg gtgagaagga acgtctcgtc gcggtgaacc caccaactgc ctgccagggc
      181 ccccagtccg acaaccatgc tcataatggt ccaggcacgg ccgacaggtg cgctgagtgt
      241 ggcgatgcgc atgaacagaa tgaactccag acctacatac acgttgtaca acgaggtatt
      301 gggcgcatgg aggagattgc gtagaaccgc ggccgacagt tctgtcgcca gggccaggcc
      361 gagcatcacg ccgatccacg gtccggcgct acccggttct cgtcgggcac gcagccagga
      421 caacatcgcg agcgatacga tcccgataag caggtgggtg gaggtcatgc acttccgcgg
      481 ggtgttcagc agccggggcg tacggcaacc ccgctatccg gaaatgctgc tttcacacag
      541 gttggcggac acgccgagcc cagatcactc ccgcgcttct cgaaggtctt ggtgttcgca
      601 gaatcggaga tcagccgttg gccggccatc tgtgccacca ggcacaggtg catgctgaag
      661 tcctctcccc ggttgtgcgc gtcccagctg accggttcgg ctatcggaac cagttcaaga
      721 tgatcagtgg gggccaattg gttgtgcgcg atcagggtgt cgatatcgga acagcggaag
      781 atcatgtgtg cgtcgagccc ttgatagcgc tgatcgaata cactatcatc cttcgtgtcc
      841 accactacac tgtcctgaaa gcgtttgccc atccaggacc gccaggcgcc catggtggta
      901 ttggccatgg ccagtttggc attggcatct atcgcgtaca agggctcgtg tatcctgtag
      961 ccgctctggc caatggtgtc cggttgggcg aggcagacca tgctgaaagc cagcgaaacg
     1021 cgcttcgcct tggcgctcgt gtcagctccg taatgcacca tcagtcccaa caggctatca
     1081 cccttgcagg tccgcgcatc gcgcagggcc acgtaactct ccaccgtgaa gcggatcacc
     1141 tgtgtatcca gcggcacatg tgtggatgag gtaccccagt gcacatgggt gccgaattcc
     1201 ccttccacac agctgagttc cttggcggt
//
LOCUS       DAOQOC010001110         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104233_length_1229_cov_2.545145, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001110 DAOQOC010000000
VERSION     DAOQOC010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104233_length_1229_cov_2.545145"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..>1229)
                     /locus_tag="PL106_14835"
     CDS             complement(28..>1229)
                     /locus_tag="PL106_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34401.1"
                     /translation="KEQVALGGCGVDFGPVYYEGMAVTRTSAIVFFRGHDPKERQGLM
                     FARKYKLPSLEPEGPAIVVAKYPFPNGRNMVSSEYRFKIYRSADDSKFLVTSLLNFPE
                     KKVHEYGCWAFNSNLEPLWDHMLHYDQPKGHRIEQSSVVITESGVAYIAFNPLSLELK
                     PDKGVPSDPAPELQPFIVRLDAQGTKQRALKLQAGHSTFGMKLVLKGDGLVAGGFECA
                     NLKKGNADAVYIWELKPDLEEKAPAVSVALNNPEEHKPRITFMVPTADGFAFAGRTLE
                     QLMLVRVNEQLVKTGEVTTPFGDIWWTMQFGNSGGNAVVTFYDYPKNIENLNTGKGLR
                     NSVGYLMEPGYCILDANGKLKCGQMVTDTDAVRDILNFRTETFRLGVYADRIHGSKPG
                     IIVTEWR"
BASE COUNT          238 a          356 c          333 g          302 t
ORIGIN      
        1 caggatgctt agctcagttt gaggtgatca tcgccattcg gtcacaatga tccctggttt
       61 gcttccatgt atgcggtccg cgtaaacacc caagcggaat gtctcggtcc tgaagttcag
      121 gatatcgcga acagcatccg tgtcagtgac catctggccg cacttgagct ttccgttcgc
      181 atcaaggatg cagtaaccgg gctccatgag gtaaccaacg gagttgcgca atcccttgcc
      241 cgtgttgagg ttctcgatgt tcttcgggta gtcatagaac gttacgaccg cattgccgcc
      301 cgagttgccg aactgcatgg tccaccagat atcgccgaag ggggtcgtca cttcgccggt
      361 tttcacgagt tgctcgttca cacgaaccag catcagctgt tccaaggtgc gccccgcgaa
      421 cgcgaaacca tccgctgtgg gtaccatgaa ggttattcgc ggcttgtgtt cctcgggatt
      481 gttcagggct acgcttacgg ccggtgcctt ttcctccaga tcgggcttga gctcccagat
      541 atacacggcg tcggcgttcc ctttcttcag gttcgcacat tcaaaccccc ctgcgaccag
      601 tccatcaccc ttcagaacca gcttcatgcc gaaggtgcta tgtccggcct gcagcttcag
      661 ggcgcgctgc ttggtgcctt gcgcgtccag ccgcacaatg aatggctgca gttcaggcgc
      721 tgggtcgctg ggtactcctt tatctggttt caactctaag gagagtggat tgaatgcgat
      781 gtacgcaaca ccgctttcgg tgatgacaac ggagctttgt tcgatcctgt gccctttcgg
      841 ctggtcgtag tgcagcatgt ggtcccatag gggttccagg ttcgaattga aggcccagca
      901 accgtactca tgcactttct tctccgggaa gttaagaagt gatgtaacca ggaacttgct
      961 gtcatcggcg cttcggtaga tcttgaaacg gtattccgag ctcaccatgt tcctgccgtt
     1021 cggaaatggg tacttggcca ccacgatcgc cggtccctct ggctccaagg aagggagctt
     1081 gtacttgcgg gcgaacatca gtccttggcg ctccttggga tcatgtccac gaaagaagac
     1141 gatggcgctc gttcgtgtca ccgccatgcc ttcatagtaa accggaccga agtccacgcc
     1201 acaaccgccc agggccacct gttccttgg
//
LOCUS       DAOQOC010001111         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104498_length_1227_cov_3.863481, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001111 DAOQOC010000000
VERSION     DAOQOC010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104498_length_1227_cov_3.863481"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PL106_14840"
     CDS             complement(<1..378)
                     /locus_tag="PL106_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNA34402.1"
                     /translation="MSKIKFGTDGWRAIIADDFTVDNVARVSVAVAKWVKQNFPKDPA
                     IVVGHDCRFAGELFAETCTKVFVHAGIKVHLAKGFVSTPMVSLGAFNLKAGMGVILTA
                     SHNPPSYNGYKLKGIYGGPLIPEH"
     gene            complement(456..>1227)
                     /gene="mltG"
                     /locus_tag="PL106_14845"
     CDS             complement(456..>1227)
                     /gene="mltG"
                     /locus_tag="PL106_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNA34403.1"
                     /translation="RVRSGTSPNALIDLLRSGEQEPVRITFNNIRTLPEFAGKVAGYL
                     EADSAALIQAIRDPSTIERLGLKPETMISLFIPDTYEMYWTDAPAEVLARMAREYKHF
                     WNVERRAKAKEIGLDQGEVATLASIVQAETAKSDEAPKIAGVYLNRLRINMPLQADPT
                     LKFALGIDSLNRVLDRDKEVDSPYNTYANTGLPPGPINMPEARYIDAVLDAAKHDYLY
                     FCARESLDGYSNFARTYEQHLVNARRYQRALNQRGIYR"
BASE COUNT          216 a          390 c          353 g          268 t
ORIGIN      
        1 gtgctcgggg atcaagggtc caccatagat gcccttcagc ttgtacccgt tatagctcgg
       61 cggattgtgg ctggcggtca ggatcacgcc catgcccgcc ttcaggttga acgcacccaa
      121 ggaaaccatc ggggtgctga cgaacccctt ggccaagtgg accttgatgc ccgcatgcac
      181 gaacaccttg gtgcaggtct cggcgaacag ctcgcccgcg aaacggcaat cgtgacccac
      241 cacgatggcg gggtccttcg ggaagttctg cttcacccac ttggccacgg ccacgctcac
      301 ccgcgccaca ttatccacgg tgaaatcgtc cgcgatgatc gcacgccagc cgtccgtgcc
      361 gaatttgatc ttgctcatcc tgcttgtttt gaacggccgc gaaagtagtc cccccccttg
      421 gccgatggtc aagtacggac cgcgatgatc aacgatcagc ggtagatacc tcgctggttc
      481 aacgcccgct gataccgccg ggcattcacc aggtgctgct cgtatgttct ggcgaagttg
      541 ctgtacccgt ccaacgactc ccgcgcgcag aagtacaaat agtcgtgctt cgccgcatcc
      601 agtacggcgt cgatgtatcg tgcttcgggc atgttgatcg ggccgggtgg tagcccggta
      661 ttcgcgtacg tgttgtaggg cgaatccacc tctttgtccc ggtcaagaac gcggttcagg
      721 ctgtcgatgc cgagcgcgaa cttcaaggtg ggatcggcct gtaacggcat gttgatcctc
      781 aagcggttga ggtacacacc cgcgatcttc ggtgcctcat ccgacttcgc cgtttcggcc
      841 tgcacgatac tcgccagggt ggccacctcg ccctggtcca gcccgatctc cttcgccttg
      901 gcccgccttt ccacattcca gaaatgtttg tactcgcgcg ccatgcgggc cagtacttcg
      961 gccggtgcat ccgtccagta catctcgtac gtatcgggga tgaacaggga gatcatggtc
     1021 tccggcttga gacccagccg ctcgatggtt gatggatcac ggatcgcttg gatcaatgcg
     1081 gccgagtccg cctccaggta tcccgccact ttcccggcga actcaggcag cgtccggatg
     1141 ttgttgaagg tgatccgtac gggctcctgt tcgccgctgc gcaacaggtc gatcaatgcg
     1201 ttcgggctgg tgccgcttct cacacgg
//
LOCUS       DAOQOC010001112         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104549_length_1227_cov_2.748294, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001112 DAOQOC010000000
VERSION     DAOQOC010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104549_length_1227_cov_2.748294"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PL106_14850"
     CDS             <1..1220
                     /locus_tag="PL106_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34404.1"
                     /translation="NLRYMWRFHESSDDMDPGSIIWTDDKPESTPLFHQFNTPGTINV
                     DLIAFDKNTYRVSDIATQRVVVKPNDPNSVHLRFWVSDSYTGRGLLPCPSTPPSAIAS
                     KYWNTDQITNPAPTGFVVYAKVDGSEVWSTDIALIDEWRHVDVDITNLIDVSLPLQQH
                     SLEIGLKAVSNVDGAEVRGVEIQLDDVSINGANGWNTLRNPGFEEEVASSFYWARFEE
                     CFTCRPSPWPECKGTPLPGFPAVTYVNPVTPTLSSMGSIRSGAYSLMMYVRAMEGWRL
                     KSGNTTTVLNYTPGMYYPPGRYKAVSQSFKYGTTYKSVVSDPSSDLIDYDAELIPNPT
                     DNSGTMIQLSALEGDPTPTYVTVIDATGRVIEQATFVGDTYKLAPIQSNGLYLVRLSR
                     GSGTLVKRLMVKH"
BASE COUNT          291 a          340 c          336 g          260 t
ORIGIN      
        1 gtaacctgcg gtacatgtgg cgcttccacg aaagttcgga cgacatggat ccagggtcga
       61 tcatttggac cgatgacaaa cccgaaagta cgccgctatt ccaccaattc aatacacctg
      121 gaacgatcaa cgtggacctc atcgccttcg acaagaacac ctaccgggtg tcggacatcg
      181 ccacgcagcg ggtggtggta aaacccaatg accccaactc cgtccatctg cggttctggg
      241 tttcggatag ctacactggg cgtggcctgc tcccttgccc aagtacacca ccaagtgcca
      301 tcgcgtccaa gtactggaac accgaccaga tcacaaatcc cgctcccaca gggttcgtgg
      361 tgtacgcgaa ggtggacgga agcgaagtat ggagcaccga catcgccttg atcgacgaat
      421 ggcgccacgt ggatgtggat atcacaaatc tcatcgatgt gagcctaccg ctacagcaac
      481 attcattgga aattggattg aaggctgttt ccaatgtgga tggcgccgag gtacgtggtg
      541 ttgagatcca actcgacgat gtgtccatca acggtgcgaa tgggtggaac acattgcgaa
      601 accccgggtt tgaagaggag gtagcgtcgt ctttctactg ggcccgtttt gaagagtgct
      661 tcacctgtcg gccttcccca tggcctgaat gcaagggaac accgttgccc ggatttcctg
      721 cggtcaccta tgtgaatccg gtcaccccga cgctctcctc catggggagc atccgcagtg
      781 gtgcgtacag cttgatgatg tatgtgaggg ccatggaagg ctggaggttg aagtccggca
      841 acacgacgac ggtcctgaac tacacgcctg gcatgtacta tcccccaggg cgttacaagg
      901 ctgtctccca gagcttcaag tatggcacca catacaaatc ggtcgtttcg gatccatcat
      961 ctgacctgat cgactacgat gccgagttga tcccgaatcc gaccgacaac tctggcacca
     1021 tgatccaatt gtcggcactt gagggtgacc cgacacccac ttatgtgacg gtgatcgatg
     1081 caactggccg ggtgattgaa caggccacat tcgtagggga cacgtacaaa cttgcaccga
     1141 tccaatcgaa tggtctttac ctggtgcgct tgagcagagg gtcaggaaca ctggtgaagc
     1201 gtctgatggt gaagcactga acacacg
//
LOCUS       DAOQOC010001113         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104721_length_1226_cov_2.736123, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001113 DAOQOC010000000
VERSION     DAOQOC010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104721_length_1226_cov_2.736123"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1226
                     /locus_tag="PL106_14855"
     CDS             <1..>1226
                     /locus_tag="PL106_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34405.1"
                     /translation="RIEYIESATTFEALNKQQLTVHQEHAMLASVHAVATVFSDHIRH
                     RVQGFPLPYYYGLPGTGKDQLIKSCQNLFGRPIPALRLPAVNTSVAQVNTFAELRSIP
                     LYLTEWMPTLKPETHAFIMGIWDGEGRKRGEKVMAGRSRFSTEDVPIHCTASLSGNYY
                     PNTMEQLLDRLLVSEMPKLTNITMDMREAYRDLNRMNEAGFSYLLADIVTHREEFTRT
                     WYAEHYTKAHDMVGVALGDQYVSERQRTNMAILLGTLLFFQKKLAWAFTPDAFCGYLV
                     RNALKQHSRRLSGDDVSNFWTCFIAAAGARQLVRDNHFSLDLSMNLVAFYWTDVFAVY
                     MEQHRRVFGAPGQRDILNKLKQHECFVRGRNGKDVHEAYRIGDRKSTAYVFDATTTGT
                     DLIGLLDTRTESVQDA"
BASE COUNT          266 a          380 c          370 g          210 t
ORIGIN      
        1 ggcgcatcga gtacatcgaa agcgccacca cgttcgaggc cctgaacaag cagcagctca
       61 ccgtacacca ggaacatgcg atgctggcca gtgtgcatgc cgtggccacc gtgttcagcg
      121 accatatccg gcaccgggtg cagggctttc cgctgccgta ttactacgga ctaccgggca
      181 ccggcaagga ccagctcatc aagtcgtgtc agaacctgtt cgggcgaccg atcccggccc
      241 tgcgcctgcc cgccgtgaac accagcgtgg cccaggttaa caccttcgct gagcttcgca
      301 gcatacccct gtacctcacg gagtggatgc ccaccttgaa gccggaaacc catgccttca
      361 tcatgggcat ctgggacggt gaaggcagga aacgggggga gaaagtcatg gccggccgtt
      421 cgcgcttcag tacggaggac gtgcccatcc actgcaccgc cagcctgagc ggcaactact
      481 atccgaacac gatggagcaa ctgctcgatc gcctattggt gagcgagatg ccgaagctca
      541 ccaacatcac gatggacatg cgggaggcat acagggactt gaaccgcatg aacgaagccg
      601 ggttcagcta cctgctggcc gacatcgtga cgcaccggga ggaattcacc aggacatggt
      661 acgcggagca ctacaccaag gcccacgaca tggtgggcgt ggcgctgggc gatcaatatg
      721 tgagcgaacg gcagcggacg aacatggcca tcctgctcgg taccctgctc ttcttccaga
      781 agaagctggc gtgggccttt accccggatg ccttttgcgg ctacctggtg cggaacgcgc
      841 tgaagcagca cagccgccgg ttgagcggcg acgatgtgag caacttctgg acctgcttca
      901 tcgccgctgc cggtgcccgc cagctcgtgc gcgacaatca tttcagcctg gacctatcca
      961 tgaacctcgt ggcgttctac tggaccgacg tgttcgccgt ctacatggag cagcaccgcc
     1021 gcgtattcgg cgcaccaggt cagcgggaca tcctcaacaa gctgaagcag cacgagtgct
     1081 tcgtgcgtgg gcgaaacggt aaggatgtgc atgaggcgta ccgcataggc gaccggaaaa
     1141 gcacggccta cgtgttcgat gcgacgacaa cgggcacgga cctcataggc ctgctcgata
     1201 cgcgcacaga gagtgttcaa gatgct
//
LOCUS       DAOQOC010001114         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_104994_length_1224_cov_4.676647, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001114 DAOQOC010000000
VERSION     DAOQOC010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104994_length_1224_cov_4.676647"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..732)
                     /locus_tag="PL106_14860"
     CDS             complement(190..732)
                     /locus_tag="PL106_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNA34406.1"
                     /translation="MEPLPEHTIQFSGLKDGQHRFAFELGDAFFAATGMEEYELGGQV
                     QATVIMDKSDHLLVTTIHVEGHANMPCDHCNAPLRQPVKGDQRQIFQLSGEAAIDDEE
                     LVGLSADAHEVNLTHYLFECISLSLPIRHVHPEGQCDPEAEAALGRVQVDEQPDGTSA
                     HEAQADPRWDALKTLKDREN"
     gene            complement(1032..>1224)
                     /locus_tag="PL106_14865"
     CDS             complement(1032..>1224)
                     /locus_tag="PL106_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34407.1"
                     /translation="LRIQLGAYAENASSDPMRASERRAQALAGHLLQNGIAMERVAAT
                     GLGAAPDPDLVDAVPITVQ"
BASE COUNT          198 a          370 c          405 g          251 t
ORIGIN      
        1 tcgccgacct ggtgcgcgcg atggcgcatg tgcaactcgc ccgagtggct gtcggtgctc
       61 agggtaggga cgcccgcttt cttggtgctg cggcggctgg ccgtgcgggt cttcgaaaat
      121 cggtgcttcg gatttggcat cgtcgtacgt gttcggtccc atcagggcca ggaccatgtt
      181 ttcgcttcct taattctcgc ggtccttcag tgttttcaag gcgtcccaac gtggatcggc
      241 ctgtgcctca tgcgcgctgg tgccatcggg ttgttcgtcc acttgtacac ggcccaaggc
      301 ggcctccgct tcgggatcgc actggccttc gggatgcaca tggcggatgg gcaggctcag
      361 gctgatgcat tcgaagaggt agtgcgtcag gttcacctcg tgcgcatcgg cgctcaggcc
      421 caccagctcc tcgtcgtcga tggcggcctc cccggagagt tggaagatct gccgctgatc
      481 gcccttcacc ggctgccgca agggtgcgtt gcagtggtcg cagggcatgt tggcgtggcc
      541 ttccacatgg atggtggtca ccagcaaatg gtcgctcttg tccatgatca ccgtggcctg
      601 tacctggccc cccagctcat attcctccat gccggtggcc gcgaagaacg cgtcgcccag
      661 ttcgaacgcg aaccggtgct ggccatcttt cagtccggag aactggatgg tatgctcggg
      721 caggggctcc atgtggactt gcttccccga ggtacgggga aagggcggca aaagtagccc
      781 aaatcggctt atccacagcg gtggaaaacg aggccaaggg tcatcggtgg cacacgcgga
      841 cgcgggaact ggaggaacgc tgacccatga gcacgtgcag tttgcctcgg ttgcgctacg
      901 gggatcgatc caaacccgcg ttcaacaggc ctgacccttt cgtccaaggg catggcaacg
      961 ccggtcacgg tcagcccgcc gaactggtcc ggcgggctga ctttgatatt cggggaactt
     1021 gagccggggg gctattgaac tgtgatcgga accgcgtcca cgaggtccgg atcaggggcg
     1081 gccccaaggc ctgtcgcggc aacgcgctcc atggcgatgc cgttctgcaa aaggtggccg
     1141 gccaacgctt gcgcacggcg ctcgctggcc cgcatggggt cggagcttgc attttccgcg
     1201 taagcgccca gttggatgcg caac
//
LOCUS       DAOQOC010001115         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105004_length_1224_cov_3.591959, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001115 DAOQOC010000000
VERSION     DAOQOC010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105004_length_1224_cov_3.591959"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..689)
                     /locus_tag="PL106_14870"
     CDS             complement(255..689)
                     /locus_tag="PL106_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34408.1"
                     /translation="MLLDTPHVLQLRKDPHVTVQADIAEGSISLAWNGFVPSGAYRRI
                     LDDALSNVCLHKLRSWASDFRYMNAILRQDERWTAEHWFPRVAAAGLQRMAIVMSDDL
                     FNRMAMERVMAEVTPQLPFAVAYFDDPEQARAWLQDRNVERL"
     gene            complement(818..>1224)
                     /locus_tag="PL106_14875"
     CDS             complement(818..>1224)
                     /locus_tag="PL106_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA34409.1"
                     /translation="ARAVDDFSKAIKQNPNYSFAFNNRGWSRFKLGDTNGAIKDIDLA
                     RSKKPDNPYIYRNLGIIALEKKDVRGACTHFQHALELGFTALHGDEVERLVKEHCPNA
                     LPPSGVRENAPSGTSVDPGTRSRTRSRSNAPE"
BASE COUNT          257 a          353 c          348 g          266 t
ORIGIN      
        1 gaaagcttgc atttgcttct cgttcatctg gacggccttg cccacggact ccaccgtggt
       61 cttgccttcg gtggcgctcg cgtggccatg gccggcacag ttttgtgccc atgtggtagt
      121 ggccatgcag gcgaaggcaa ggccagcgat caaattcaac gctttcatca ggttgtgttg
      181 tttgggttgc gggtgcgtgt ctatgctcgt tggggaccaa agtaacaaag aggaccccgt
      241 agaaccacac gtattcacaa cctttccaca ttccggtcct gcagccaagc gcgggcctgc
      301 tccggatcgt cgaaataggc cactgcgaat ggcagctgag gcgtcacttc ggccatcaca
      361 cgttccatgg ccatccggtt gaagagatca tcgctcatca cgatggccat ccgctgcagg
      421 ccggcagcag ccacccgtgg gaaccaatgc tcggccgtcc agcgttcgtc ctgccgcagt
      481 atggcgttca tgtaccggaa gtccgatgcc caggatcgca gtttatgcaa acaaacattg
      541 ctcaaagcat cgtccaggat acggcgatag gccccactgg gcacgaaacc gttccatgcc
      601 aacgagatgg aaccctcggc gatgtcagcc tgaacggtaa catgggggtc cttccggagc
      661 tgaaggacat gaggtgtatc aaggagcatg gtgcaaagat ggcgataagc cagaacccga
      721 atatggacca accttggatt ttcaacgacg aaccggcttc cgatcccgac catcgcggaa
      781 tatgccacct cgacagaacc gggttttgga cctcccgcta ctccggcgcg ttggaacgcg
      841 accgtgttct ggagcgggtc cccggatcca ccgaagttcc ggaaggagcg ttctcccgaa
      901 ccccagaagg cggaagtgcg ttgggacaat gctccttcac caagcgttcc acttcatcgc
      961 cgtgcagagc ggtgaacccc agttccaatg catgctggaa atgggtgcat gcccctcgaa
     1021 catccttctt ttcaagcgct ataattccta aatttcgata gatataagga ttgtcgggct
     1081 ttttggaccg ggccagatcg atgtccttga tcgctccgtt cgtgtcgccg agcttgaacc
     1141 ggctccaacc gcggttgttg aaggcgaagc tgtagttggg gttctgcttg atggccttgc
     1201 tgaaatcgtc gacggcccgg gcgt
//
LOCUS       DAOQOC010001116         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105055_length_1224_cov_2.726262, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001116 DAOQOC010000000
VERSION     DAOQOC010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105055_length_1224_cov_2.726262"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1223)
                     /gene="fmt"
                     /locus_tag="PL106_14880"
     CDS             complement(249..1223)
                     /gene="fmt"
                     /locus_tag="PL106_14880"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNA34410.1"
                     /translation="MHPPQNMPQRIVFMGTPPFATASLAALVEAGFDVAAVVTAPDRP
                     AGRGQQLRMSAVKEYALAHPVLRDRILQPEKLKDPDFHARLDATGASLYVVVAFRMLP
                     EVVWKRPVLGSINLHASLLPDYRGAAPINWAVINGETRTGVTTFFIRQEIDTGDVIGR
                     EETSIGPDETAGEVHDRLMAIGARLVVRTVGNILAGRASPVPQSDSPALHTAPKLTPD
                     NCRIGWSGSARNVHDHIRGLSPWPGAWSKLIIGDRPEQHFKILNTRVVANAGNGQAGR
                     VEWIDERFIIHCGSGTIEALELQAEGKKRMAAASFMRGLQGEPALRFI"
BASE COUNT          231 a          381 c          365 g          247 t
ORIGIN      
        1 agtcggccgg aaagccgctg atatcaaggg tttcagaata tactccatga gccgttcgta
       61 cgaagtgttg ggccaacggc ttggtggccg ttccacacct ccgcgatcga tcatgcgtac
      121 gcgcaccgca ttccatccaa cgttcatcgc ctgtgtcgca cggccggacg actgaacgtc
      181 gaaccgttca gcggttgcgg tgaggagtgt tggctgcttg ttccgtgtgg cacacccgtg
      241 atcgtcgatc aaatgaaccg aagcgcaggt tcaccttgta gtccgcgcat gaacgaagcg
      301 gcagccatcc gcttcttccc ttcggcctgc aactccagtg cttcgatcgt tccactgccg
      361 cagtggatga tgaatcgttc gtcgatccat tctacgcggc ctgcttggcc attgccagcg
      421 ttcgcgacca cccgggtgtt gagtatcttg aaatgttgtt cgggccgatc accgatgatc
      481 aacttggacc atgcaccggg ccatgggctg agccctcgga tgtgatcatg cacgttcctg
      541 gcggatccgc tccatccgat ccggcaattg tccggggtca gtttcggcgc cgtgtggagc
      601 gctggcgagt ccgactgggg tacgggtgat gcacggcctg caagaatgtt gcccacggtt
      661 ctcaccacca accgggcgcc gatggccatc aatcggtcat gcacctcacc ggcggtctca
      721 tccggaccga tagaagtctc ctcgcgaccg atcacatcgc cggtatcgat ctcctggcgg
      781 atgaagaagg tggtgacccc ggtccgggtc tccccattga tcaccgccca gttgatcgga
      841 gcagctccgc gataatcggg cagcaaggag gcgtgcaggt tgatcgatcc gagcaccggc
      901 cttttccaca ccacctccgg cagcatccgg aaggcgacca ccacatacaa cgatgcaccc
      961 gtcgcatcga gacgcgcgtg gaagtcggga tctttcaatt tttcgggttg aaggatgcga
     1021 tcgcgcagaa ccgggtgggc cagcgcgtac tccttcacgg cactcatgcg caactgctgc
     1081 ccacgtccgg cgggcctgtc cggcgcggtg accacggccg ccacatcaaa acccgcctca
     1141 acgagcgcgg cgagcgatgc cgtggcgaac ggaggcgttc ccatgaagac gatacgctgt
     1201 ggcatgtttt gaggaggatg cacg
//
LOCUS       DAOQOC010001117         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105391_length_1222_cov_3.055698, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001117 DAOQOC010000000
VERSION     DAOQOC010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105391_length_1222_cov_3.055698"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PL106_14885"
     CDS             complement(<1..1190)
                     /locus_tag="PL106_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNA34411.1"
                     /translation="MNQSTYQIVPGDQVLFKRGGEYRGEIIWGVSGTAGSPVTYGAYG
                     TGNDPIISGAKLVTNWTIHSGNIYKAYVGMQVDYVYIGGSRATLARTPNTGWYRNSNG
                     QNNTMTSANITQSSGYFTGARCILRSTASSVDTLRITNHSGSTLTFGNNPTNSSMGQD
                     DWGFFVENKLNLLDAAGEWFYDKSNGYLYLWAPGSANPNSLTVEASVYESGVNCYWQR
                     HYCTIQNLYFRYQRYAGVLNASADHVTVTGCTMEDVYYGIRSYGSYDNYNNNIIRRTY
                     ASGCLMIDNNSVFENNTLSDIAMILGMGENSWGYFGIRGIGQSNTIRNNSFTNVGYIA
                     IVADGNQLIEKNRVSNCSVALNDGGAIAFDNANGMIIQDNILGDAIGGLDGSSTVLPH
                     YQHLG"
BASE COUNT          210 a          345 c          381 g          286 t
ORIGIN      
        1 ccgaggtgct ggtagtgggg cagcaccgtg ctcgatccgt ccagtccgcc gatggcatca
       61 ccgaggatgt tgtcctggat gatcatcccg ttggcgttgt cgaaggcgat ggccccaccg
      121 tcgttgagcg ccacgctgca gttgctgacc ctgttcttct cgatcagctg gttgccatcg
      181 gccacgatgg cgatgtagcc cacattggtg aagctgttgt tgcggatcgt gttgctctgg
      241 ccgatacccc ggatgccgaa gtaaccccag gaattctcgc ccatgcccag gatcatggcg
      301 atgtccgaga gcgtgttgtt ctcgaacacc gaattgttgt cgatcatcag gcagccactg
      361 gcgtaggtgc ggcggatgat gttgttgttg taattgtcat agctcccgta gctgcggata
      421 ccgtagtaca catcttccat ggtgcaaccc gtgaccgtta cgtggtccgc cgaggcgttg
      481 agcacaccgg cataacgctg ataccggaag tacaggttct ggatggtgca gtaatggcgc
      541 tgccagtagc aattgacccc gctctcgtac accgaggcct ccacggtgag gctgttggga
      601 ttggcgctgc ccggtgccca gaggtagagg tagccgttgc tcttgtcgta gaaccactcg
      661 ccggcggcat cgagcaaatt caatttgttc tctacgaaga aaccccagtc gtcctgcccc
      721 atgctgctgt tggtggggtt gtttccgaag gtgagggtac tgccgctgtg gttggtgata
      781 cgcagcgtgt ccacactgct ggccgtgctg cgcaggatgc aacgggcacc ggtgaaatag
      841 ccgctggact gcgtgatgtt ggcactggtc atcgtgttgt tctgaccatt gctgttgcgg
      901 taccagcccg tgttgggcgt gcgggccagg gtggcccggg atccgccgat gtacacgtag
      961 tccacttgca tgcccacgta ggccttatag atgttgcccg agtggatggt ccaattggtg
     1021 accaacttgg caccactgat gatgggatca ttgcccgtac cgtacgcgcc ataggtcacc
     1081 ggggatcctg cggtgccgga tacgccccag atgatctctc cgcggtactc ccctccccgt
     1141 ttgaagagga cctggtcacc cggcacgatc tgataggtgc tctggttcac gcgatcgatc
     1201 gtacgccacg gcgtgctctg gg
//
LOCUS       DAOQOC010001118         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105486_length_1222_cov_1.982862, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001118 DAOQOC010000000
VERSION     DAOQOC010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105486_length_1222_cov_1.982862"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1222)
                     /locus_tag="PL106_14890"
     CDS             complement(<1..>1222)
                     /locus_tag="PL106_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34412.1"
                     /translation="TGCSTQNITWLHGGTSGLFNLEYSTDNGAPWTAIVSNYNGGAGP
                     SSSYSWQVPNIGSVQALVRITDAGDPLKTDVSNSVFTMTQTTNIVLLTPNGNENWVTG
                     TTQAITYSVGGGVTTVRLEYSTNGGSTWTTIVTSTSGGVYNWTIPNTPSATCLVRATD
                     TGNSCNSDVSNANFVIVSQVTVNVPNGGEVWQATVGNQGISYNMDNVPVTMNTGNFYD
                     PGGLASNYTYTGATLTKTFTPDIPTNKLRVVFNSFSTYNGADQFRIYNGPSTASPLIG
                     TYTGSTIPPAFTSTHSSGALTFTWYTGSGNVSSGWDAVISSVGTPTQAVNWSIVGTSQ
                     YFNLDYSTNAGTSWIRILTNYPSSTGNFAWSVPNTPTTQARFRVMDYNNNAILDQSNA
                     NFTISPYVTVTAPNG"
BASE COUNT          217 a          318 c          401 g          286 t
ORIGIN      
        1 ccattgggtg ccgtcactgt gacgtaaggc gagatggtga agttggcgtt gctctggtcc
       61 aggatggcgt tgttgttgta atccatcacc cggaaacgcg cctgcgtggt gggcgtattc
      121 gggacggacc aagcaaaatt gccagtgcta gagggatagt tggtcaggat gcggatccag
      181 cttgtccctg cattggtgct gtaatcgagg ttgaagtact gggaggtgcc tacaatgctc
      241 cagttcacgg cctgcgtcgg agtccctaca ctgctgatca cggcatccca ccccgacgag
      301 acgttgcccg aacccgtgta ccaggtaaag gtcaatgctc cactgctgtg cgtggatgtg
      361 aatgctggtg ggatggtgga gcctgtatag gtaccgatca ggggagatgc agtgctcgga
      421 ccattgtaga tcctgaactg atccgcccca ttgtaggtgc tgaacgaatt gaagaccacc
      481 cggagcttgt tggtggggat gtccggggta aaggttttgg tcaacgtggc acctgtgtaa
      541 gtatagttgc ttgcgagacc tcccggatca tagaagttgc cggtattcat cgtcaccgga
      601 acgttgtcca tgttgtagga gatgccttgg ttgcccacgg tcgcctgcca gacttcgccg
      661 ccgttgggga cgttgacggt cacttgcgac acgatcacga agttggcatt gctcacgtcg
      721 ctgttgcagg agttacctgt gtccgtcgca cgcacgaggc aggttgcgct cggggtgttg
      781 gggatggtcc agttatagac cccgccgctc gtgctcgtga cgatggtggt ccaggtggag
      841 ccgccgttgg tgctgtattc caggcgcacc gtggtcacgc cgccgcccac gctgtaggtg
      901 atggcttgtg tggtgccggt cacccagttc tcgttgccgt tcggtgtcag cagcacgatg
      961 ttcgtggtct gggtcatggt gaacacggaa ttgctcacgt cggtcttcag cgggtcgccc
     1021 gcatccgtga tccgcaccag ggcctgcacc gaaccgatgt tcggaacctg ccaagaatag
     1081 gaggagctgg ggcctgcgcc gccattgtaa ttgctgacga tcgcggtcca gggcgcgccg
     1141 ttgtccgtgc tgtattcgag gttgaacaag ccggaggtgc cgccatggag ccaagtgata
     1201 ttctgggtgc tacaacccgt cc
//
LOCUS       DAOQOC010001119         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105587_length_1221_cov_3.068611, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001119 DAOQOC010000000
VERSION     DAOQOC010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105587_length_1221_cov_3.068611"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PL106_14895"
     CDS             <1..708
                     /locus_tag="PL106_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNA34413.1"
                     /translation="GRIEHFVGRKMMAMDGLGGETVVELVRTGLIHDVADLYDLKKEQ
                     LLALGKGWGEKSAAQVIEGIAASKAVPFEQVLFALGIRHVGETVAKKIARAVGSIDRL
                     MTMPKEELLSLDEVGDVIAQSITDFFAAAGNRRIVERLRLAGLRFQVEPSQQHVAGDK
                     LKGLTFVVSGVFSGYDRDGIKEAIERNGGKVSGSISKKTSFVLAGADMGPAKRQKAEE
                     LGVKIIGEEEFDRMIGA"
     gene            705..>1221
                     /locus_tag="PL106_14900"
     CDS             705..>1221
                     /locus_tag="PL106_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34414.1"
                     /translation="MKLFDRIKEWLGLRRLLKETMPDRRPVPKNLARCVKVGIVYLAT
                     DEKAHLDVRNYVKKLKEDLGLVKIQCIGYFDGKELPFHLHAKLNFDAFCQKDLNWYRI
                     PSGNVVDNFIAEEHDVLLDLTLDDHLPLRYIVAKSRARFKVGRFSEGNKRFLDMMIDT
                     AGARSLPQFIAN"
BASE COUNT          263 a          335 c          423 g          200 t
ORIGIN      
        1 gggcgcatcg agcactttgt cggccgcaag atgatggcca tggacgggct gggtggcgag
       61 accgtggtgg agctggtgcg caccggactc atccacgacg tggccgacct gtacgacctg
      121 aagaaggaac aactcctcgc actgggcaag ggctggggcg aaaagagcgc cgcgcaggtg
      181 atcgaaggga tcgcggcgag caaggccgta cctttcgagc aggtgctctt tgccctgggc
      241 atacggcacg tgggcgagac cgtggccaag aagatcgcgc gggccgtggg ctccatcgac
      301 cggctgatga ccatgccgaa ggaggaactg ctctcactgg acgaagtggg cgacgtgatc
      361 gcgcagagca tcaccgactt tttcgccgca gcgggcaacc gacggatcgt ggaacggttg
      421 cgcctggcgg gcctgcggtt ccaagtggaa ccgtcgcagc agcatgtcgc aggcgacaag
      481 ctcaagggcc tgaccttcgt ggtgagcggg gtgttcagcg gctacgatag ggacgggatc
      541 aaggaggcca tcgagcggaa cgggggcaag gtgagcggat cgatcagcaa gaagacgagc
      601 tttgtgctcg ccggggccga catgggcccc gccaaacgac aaaaggccga ggaactcggc
      661 gtgaagatca tcggagagga ggaatttgat cgtatgatcg gggcgtgaaa ctgttcgacc
      721 gcatcaagga atggctcggc ctgcgtcgcc tgctgaagga gacgatgccc gaccggcgcc
      781 ccgtgcccaa gaacctggcg cgctgtgtga aggtgggcat cgtatatctg gcgacggacg
      841 agaaggcgca cctcgatgtg cggaactacg tgaagaaact gaaggaggac ctgggcctgg
      901 tgaagatcca gtgcatcggt tatttcgacg gcaaggaatt gccgttccac ctgcacgcca
      961 agctgaactt cgatgccttc tgccagaagg acctcaattg gtaccggatc ccgagcggta
     1021 atgtggtgga caatttcatc gccgaggagc acgatgtgct cctggacctg acgctggatg
     1081 accatctgcc gctgcggtac atcgtggcca agagccgggc acgcttcaaa gtggggcgct
     1141 tttccgaagg caacaagcgc ttcctggaca tgatgatcga cacggccggc gcacgcagcc
     1201 tgccgcagtt catcgccaac a
//
LOCUS       DAOQOC010001120         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105674_length_1221_cov_2.212693, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001120 DAOQOC010000000
VERSION     DAOQOC010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105674_length_1221_cov_2.212693"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PL106_14905"
     CDS             complement(<1..554)
                     /locus_tag="PL106_14905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA34415.1"
                     /translation="MEHTLQALTPGTSHAWYRALYTEHRGAFVQWAARQYGVREEEAR
                     DAFQEAVIVLYRKAQGDDLASLNCSLRTYLFAVGRNQLLSRMRVLRKQEDLHEEFHGI
                     PDEALATNGMERQEKEATAELVRQRLAELKPDDRRVLELYYLEGKDMDAVAEALGLKN
                     RNVAKKKKHFALQRLIALVRSAKML"
     gene            627..>1221
                     /locus_tag="PL106_14910"
     CDS             627..>1221
                     /locus_tag="PL106_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNA34416.1"
                     /translation="MNRSALILPLLMALAPARAQQVFTNQIATSGLPSAQGNVAAIYD
                     HDNDGLLDLVWASGNPAQRSLMRNNGDLTFTDISAVSGFTDPSGSINPVDIDNNGWTD
                     IICMDWAANTITLWLNNGGYFATQLIQLQVDLHHGFSLIDLDLDGAVDILSSVASGAQ
                     IHLMVGHNDVCVMGPEASFQNWTTIAGPFQVPQGAVMI"
BASE COUNT          235 a          381 c          359 g          246 t
ORIGIN      
        1 agcattttgg ccgagcgcac gagggcgatc aggcgttgca gagcgaagtg cttcttcttc
       61 ttggccacgt tgcggttctt caggcccaag gcctcggcca cggcgtccat gtccttgccc
      121 tcgaggtagt acagttccag tacgcggcgg tcgtccggct tcagctcggc gaggcgctga
      181 cgcacgagtt cggcggtggc ttccttttcc tggcgctcca tgccattggt agcaagcgcc
      241 tcgtccggga taccatggaa ctcctcgtgc aggtcctcct gtttgcgcag cacacgcatg
      301 cgcgagagca gctggttgcg ccccacggcg aagagatagg tgcgcaacga gcagttcagc
      361 gatgccaggt cgtcgccttg tgctttgcgg tacagcacga tcaccgcttc ctggaaggca
      421 tcacgggctt cctcttcccg cacaccgtat tgccgcgcgg cccactgcac aaaggcgccg
      481 cggtgctcgg tgtagagcgc gcggtaccag gcgtgcgatg ttcccggagt gagggcctgg
      541 agggtgtgct ccatgggtgg gcaaggtagg gggtggtaac ctaccgcacg gctgttctac
      601 taaaaggtaa cctatgcccc gcacccatga accgctccgc cctcattctg cctctgctca
      661 tggccctcgc gcccgcacgc gctcaacagg tcttcaccaa ccaaatagct accagcggcc
      721 ttccctccgc ccaaggtaac gtggcagcca tctacgacca tgacaacgac gggctgttgg
      781 atctggtctg ggcttccgga aacccggcac aacgcagcct gatgcggaac aacggcgatc
      841 tgacctttac ggatatttcc gccgtgtcag gattcacaga ccccagcggc agtataaatc
      901 ctgtggacat cgacaacaac ggatggaccg acatcatctg catggactgg gcggccaata
      961 cgatcacgtt gtggttgaac aacggtggct acttcgccac gcaattgata caactccaag
     1021 tggatttaca ccacggattc tcgctcattg acctcgacct ggacggtgcc gtggatatcc
     1081 tgagctcggt agcgagcggc gcccagatcc atctgatggt cgggcataac gacgtgtgcg
     1141 ttatgggacc cgaagcctcc ttccagaact ggacgaccat cgcggggccc tttcaagtgc
     1201 cgcaaggagc agtgatgata c
//
LOCUS       DAOQOC010001121         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105778_length_1220_cov_3.218884, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001121 DAOQOC010000000
VERSION     DAOQOC010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105778_length_1220_cov_3.218884"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PL106_14915"
     CDS             complement(<1..473)
                     /locus_tag="PL106_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNA34417.1"
                     /translation="MEQRKTSWAEPYKIKMVELIAMTSREQREAALVEAGYNTFLLPS
                     EKVYIDLLTDSGTSAMSDRQWAGMMMGDEAYAGSRNFYHLEKAIQDTYGYKYIVPTHQ
                     GRGAENILSQIMIKKGQFVPGNMYFTTTRLHQELAGGTFVDIIIDEAHDPNNTFA"
BASE COUNT          211 a          364 c          383 g          262 t
ORIGIN      
        1 aaggcgaagg tgttgttggg gtcgtgggcc tcgtcgatga tgatatccac gaaggtgcca
       61 ccggccagct cctggtgcag gcgtgtggtg gtgaagtaca tgttgccggg cacgaactgc
      121 cccttcttga tcatgatctg cgagaggatg ttctccgccc cgcggccctg gtgggtgggc
      181 acgatgtact tgtagccgta cgtgtcctgg atggccttct ccaagtggta gaagttgcgg
      241 ctgccggcgt atgcttcatc ccccatcatc atgccggccc actgccgatc gctcatggcg
      301 ctggtgccac tgtcggtgag caggtcgatg tacacctttt cggagggtag caggaaggtg
      361 ttgtatccgg cctccaccaa tgcggcctcg cgctgttcgc gtgaggtcat ggcgatcagc
      421 tccaccatct ttatcttgta tggttcggcc caggatgtct tgcgttgttc catgtggggt
      481 tgatccgttc gcgttccgcg ttcaacggga gtgcgaaggt ccgggaggct ctgcccttga
      541 ggactgacgc gcgtcatgtg ggggccttgc caccgcggaa tgcccctcgc cacatggcgg
      601 ttccaacccg gcttctttac atttccatta cactggccgt tctggtcgat cgtgccgcag
      661 cggctccgat cggcaagccc ccacggatcc ggtgcatgcc gacgcgatca ggtaggccga
      721 acaggtgtac cgggatgccg atcccgggac caccccgggc catcgcgtca tggtaaggtt
      781 cgctgaaggc tatccaacga acggtctgct ggggatcgcc cgctgggatc cacgtgaggg
      841 cccatggcca tgaaattcga acggtacgcc aagtatggcg aagcacacgg agcattgcac
      901 gacttgctgt tccccttgat ccgcagggtc aagcacatcc cggataaccc ctcccgatcg
      961 cacctggaca gcttgccgca acatggccgc caatccgatc aacagttcgg ctgatcgagc
     1021 ggtgggatcc acaccgcctg tgtggggatc cgcacgacag acgggggtgg tggccctgtc
     1081 cgggatgcga tcttgatgga tatcatactt cgtaaccacg tgctcagggc aacttcggcg
     1141 cacggcccgg ggacacgtac tgatgccatc gcatcgtaga gcatgggttt ccgcccgcca
     1201 tgcatgagtg acagacgtac
//
LOCUS       DAOQOC010001122         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105904_length_1219_cov_3.923540, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001122 DAOQOC010000000
VERSION     DAOQOC010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105904_length_1219_cov_3.923540"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PL106_14920"
     CDS             <1..505
                     /locus_tag="PL106_14920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA34418.1"
                     /translation="WIRVTHHDILDPSGQQGIYGTIHFKNLAIGAIPLDDDLNTWIVG
                     QYRYPIEAYSWEIPEGGGDRNIAPLASAQRELREEAGIEAAHWQEILRMDLSNSASDE
                     HAILYVARGLTLHPPRPDHDEELELRKLPFEELYQMVLRGDVRDSLTVAGVMRVKLML
                     LDGSLQK"
     gene            complement(496..>1219)
                     /locus_tag="PL106_14925"
     CDS             complement(496..>1219)
                     /locus_tag="PL106_14925"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNA34419.1"
                     /translation="LIQLDHAWYSRYERSAFHFFDDGEEWMAMDKVGHAYSTYTVGMW
                     GHGLMRWCRFPRKTSVWVGGSVGLVYLTAVEVLDGTSSAWGFSGWDMVANAAGTGLFI
                     GQELGWGEQRIRMKYSAHLTSYAVQRPELLGEKLPERILKDYNGCTFWLSVNPNAFGW
                     KGFPAWLNLAGGYGAEGMLGSRDPGAWRQFYFAPDIDLTRIRVKSRFLRTVFFALNAV
                     KFPLPAIEFGDNGKVRVHALYF"
BASE COUNT          258 a          409 c          351 g          201 t
ORIGIN      
        1 gtggatccgg gtgacgcacc acgacatcct cgaccccagc ggacagcagg gtatctacgg
       61 aacgatccat ttcaagaacc tcgcgatcgg ggcgataccg ctggacgacg acctgaacac
      121 ctggatcgtg ggccaatacc gctatcccat cgaagcgtac agctgggaga tccccgaagg
      181 gggcggagat cgcaacatcg ctccgctcgc aagcgcgcaa cgggaactga gggaggaggc
      241 gggtatcgaa gcggcacact ggcaggagat cctgcgcatg gacctgagca acagcgccag
      301 cgatgaacac gccatcctct acgtggcccg cggactcacc ctccacccgc cccggcccga
      361 ccacgacgag gagctggaac tgcggaaact gcctttcgag gagctttatc agatggtgct
      421 gcggggcgat gtgcgcgaca gcctgacggt ggccggtgtg atgcgggtga aactcatgct
      481 gctggacggc tcgcttcaga aatagagggc atgcacccgc accttgccgt tgtcgccgaa
      541 ctcgatcgcg ggcaggggaa acttcactgc attcagcgcg aagaacaccg tgcgcaggaa
      601 gcggctcttg acccggatcc gcgtcaggtc gatatccggc gcgaaataga actggcgcca
      661 ggcgcccgga tcgcgtgaac ccagcatgcc ctcggcgccg tatccgccag ccaggttgag
      721 ccaggccgga aagcccttcc aaccgaatgc gttgggattc acgctcaacc agaaggtgca
      781 accattgtag tccttcagga tgcgctccgg caacttttca cccagcaact cggggcgttg
      841 caccgcatat gatgtcaggt gcgcggagta tttcatccgg atgcgctgct caccccaacc
      901 gagttcctgc ccgatgaaca agcccgtacc cgctgcgttg gccaccatgt cccaaccgct
      961 gaaaccccac gcactgctgg tgccgtccag cacttccacc gccgtcaggt acaccagtcc
     1021 cacactaccg cccacccata ccgatgtctt cctcgggaag cggcaccagc gcatgagccc
     1081 atgcccccac atgccgaccg tgtatgtcga gtacgcgtgc cccaccttgt ccatggccat
     1141 ccactcctcc ccgtcatcga aaaaatggaa tgcgctccgt tcgtagcggc tgtaccaggc
     1201 atggtccaac tggatgagc
//
LOCUS       DAOQOC010001123         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_105934_length_1219_cov_3.283505, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001123 DAOQOC010000000
VERSION     DAOQOC010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105934_length_1219_cov_3.283505"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1013
                     /locus_tag="PL106_14930"
     CDS             138..1013
                     /locus_tag="PL106_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA34420.1"
                     /translation="MGALSYYLALPFIYGIALLPFPLLYGVSDGLYLLLYKLIGYRTK
                     VVRENLRKSFPEKSVAELRSIERSFYRWFCDLVVETLKTLTITPAQVKERVAVEGEEV
                     LKKYFDAGRSVILVMGHWGNWELGGARFSQLGVHQLYVIYHPLQNARFDKLVEHMRTR
                     LGNRLYAMNDTLKGMLRDRGQVTATAFIADQTPPPDRAYWTTFLHQDTPVFFGTEKIA
                     GKLGYPVIYTGIERVRRGYYLMRFEELVADPKGLPEGAISELHTRRLERDIQRQPAFW
                     LWTHRRWKHRRPSGV"
     gene            1025..>1219
                     /locus_tag="PL106_14935"
     CDS             1025..>1219
                     /locus_tag="PL106_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34421.1"
                     /translation="MLQGKDLILATRPFANEVRWKSWWHLFTTLVVLGGGYAMVFVAD
                     SWWVKVGGSILVALTLGRFFV"
BASE COUNT          221 a          368 c          395 g          235 t
ORIGIN      
        1 gaaatattat cagacaggtg tcctcaggca cttctttttc aaggaggtcg gactttgcat
       61 gcgttgtctg cgggattgac gcgctcggcc gtacagcccc accctggcgg agcacagggc
      121 accgatcttc gccccgcatg ggcgccctca gctactacct ggcccttccg ttcatctacg
      181 gcatcgcact cctgcccttc cccttgctgt atggggtgag cgacgggttg tacctgctgc
      241 tgtacaagct gatcggctac cgcacgaagg tggtgcggga gaacctgcgc aagagcttcc
      301 cggagaagag cgttgcggag ttgcgaagca tcgagcgctc cttctaccgc tggttctgcg
      361 atctggtggt ggaaacgctg aagaccctca cgatcacccc ggcccaggtg aaggaacgcg
      421 tggccgtgga aggcgaagag gtgctgaaga aatacttcga tgcggggcgg agcgtgatcc
      481 tggtgatggg ccactggggc aattgggaac tgggcggcgc acgcttcagt cagctcggag
      541 tgcatcaact gtacgtgatc taccacccgc tccagaacgc tcggttcgat aagctggtgg
      601 aacacatgcg cacgcggctg ggcaaccggc tctacgccat gaacgatacg ttgaagggca
      661 tgctgcggga ccgggggcag gtaacggcca ccgccttcat cgccgaccag accccgcccc
      721 ctgaccgggc gtattggacc acctttctgc accaggacac cccggtgttc ttcggcacgg
      781 agaagatcgc gggcaaactg ggttatccgg tcatttacac cggcattgag cgggtgcggc
      841 gcgggtacta cctgatgcgc tttgaggaac tggtggccga tcccaaaggc ctgcccgagg
      901 gcgcgatcag cgagctgcac acccggcggc tggaacgcga catccagcgc caaccggcgt
      961 tctggctctg gacccaccgc cggtggaaac atcgacgtcc ttccggtgtg tagattcgcc
     1021 gccgatgctc cagggaaagg acctcattct cgccacgcgg cccttcgcca acgaagtgcg
     1081 ctggaaaagc tggtggcacc tgttcaccac gctggtagtg ctcgggggcg gctacgccat
     1141 ggtcttcgtg gccgatagtt ggtgggtgaa ggtgggcggc agcatcttgg tggccctcac
     1201 gctggggcgc ttcttcgtg
//
LOCUS       DAOQOC010001124         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106104_length_1218_cov_4.061049, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001124 DAOQOC010000000
VERSION     DAOQOC010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106104_length_1218_cov_4.061049"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /gene="tsaB"
                     /locus_tag="PL106_14940"
     CDS             <1..764
                     /gene="tsaB"
                     /locus_tag="PL106_14940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNA34422.1"
                     /translation="VQLFAGAWIARYPARAAHLLLIVALILAFDTATKHCAVVLARDG
                     AIVAQRASDEERFSHAEKLNVFIEEVLIEAALTMKDVEAVGVGIGPGSYTGLRIGLSA
                     AKGLCFALDVPLLALPTLDVLGAELLATGMAVNDTDALHPMIDARRMEVFTGVLDAYG
                     HPLGHTTPVILDDAWRNDLSTGQKHIVFGDGADKAAALWEQWIHITHVAGVRPSVRGL
                     AARSLKAFQRKEYAELARLVPQYGKEANVTRPGAK"
     gene            957..>1218
                     /locus_tag="PL106_14945"
     CDS             957..>1218
                     /locus_tag="PL106_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA34423.1"
                     /translation="MIERQLSSALNARMDDRKAIVVLGPRQTGKTTLIETLLKGRESV
                     LVLNCDDPEVRGRLDRITLAGWRDLLGASKVVFIDEAQRIPDI"
BASE COUNT          234 a          349 c          396 g          239 t
ORIGIN      
        1 aagtacaatt atttgctggc gcgtggatcg ctcgatatcc tgcaagggct gcccatctcc
       61 ttctgatcgt ggccctgatc ctcgcgttcg ataccgccac gaagcactgt gccgtggtgc
      121 tggcgcgcga tggcgccatc gttgcccaac gcgcatcgga tgaggaacgt ttcagccatg
      181 ctgaaaagct gaacgtgttc attgaggagg tgctgatcga agcagctctt accatgaagg
      241 acgtggaggc ggtgggcgtt ggcatcggcc caggctcgta cacgggcctg cggatcggcc
      301 tctcggccgc gaaaggcctc tgtttcgccc tggatgtgcc tttgctcgct ttgcccacgc
      361 tggatgtgct cggcgcggaa ctgctcgcga ccgggatggc cgtgaatgat accgatgccc
      421 tccatcccat gatcgatgcg cgcaggatgg aggtcttcac cggggttctg gacgcttacg
      481 gtcatccgct gggccacacg acccccgtga tcctggacga cgcttggcgc aacgacctgt
      541 ccaccgggca gaagcacatc gtattcggcg atggggcgga caaagcggcc gcgctttggg
      601 aacagtggat ccacatcacg catgtggcgg gggtgagacc ttcggtgcgt ggccttgcgg
      661 cccggtcgct caaggcattc caacgcaagg agtacgcaga actcgcacga ctggtaccgc
      721 aatacgggaa ggaggcgaat gtgacccggc ccggcgcgaa gtgagcacat gttcgtgggt
      781 gctggtgccc gggcttgcag cgcaggtgtg tgggaagagc ggttacaccg ctcacattcc
      841 gtgggtgccc ccaacgccgt tcccgtaggt gacgcccttc ccgcaagccc tgaacaagct
      901 tgatccatgt atgtggtctc cgaaaaaagc atatattcgg agaccacaat cgccaaatga
      961 ttgaacgaca actctcttcg gccttgaacg cacgcatgga tgaccgcaag gccattgtgg
     1021 tattggggcc acggcagacc gggaagacca ccctgatcga gacgttgttg aagggtcggg
     1081 aaagcgtgct cgttctgaat tgcgatgatc cggaggtgcg cggcaggctg gacaggatca
     1141 cccttgcggg ctggcgggat ctgctcgggg cgagcaaagt cgtgttcatc gacgaggcgc
     1201 agcgtatacc ggacatcg
//
LOCUS       DAOQOC010001125         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106275_length_1217_cov_3.856282, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001125 DAOQOC010000000
VERSION     DAOQOC010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106275_length_1217_cov_3.856282"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..976)
                     /locus_tag="PL106_14950"
     CDS             complement(266..976)
                     /locus_tag="PL106_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34424.1"
                     /translation="MDDGYLVPWILRVHAIAGGIALVVAPLAMALPKGGTWHRRWGKA
                     YFYSMVVVCITAVVLGIAHYQRSFWLALVGVFSFHLVASGYRSLYLKKLHEGLKPQPT
                     DLWLHGIAGTVNGGLLIWGLAHLLMGQRDTKAMLFTVFGAIGSYMVVIQLHRFYKRKH
                     DKREWFYGHISGMLGAYIATLSAFSAVNLEMIRPTWLKWLWPTLVGVPLIILWTGHYR
                     RRFAKGARVRDMAEVRIR"
BASE COUNT          262 a          377 c          374 g          204 t
ORIGIN      
        1 ggctcagcca gacctgcagg ccgttatcga gcgtgtagat ccgggtcccc atcgggtcgc
       61 cgggtacgct cacataggtg tacgtcttgg cggctgatcg cgtcggggcg atccgggtct
      121 gggcaacggc ggaaaaagca cacgtggccg cgaggagaag gaaggattgc ttcatggggt
      181 gaccgataag ggggcgtgaa gatgcgaagg aagcaccgca ccacatcacc gaccagcccc
      241 tgggccgttg gccccacgtc ccgcctcagc ggatcctcac ttcggccatg tcgcgcaccc
      301 gggccccctt ggcgaaacgc cgacggtaat ggccggtcca aaggatgatc aacggaactc
      361 cgacgagggt aggccacagc catttcaacc acgtgggacg gatcatctcc agattcaccg
      421 cactgaacgc gctgagcgtg gcgatgtacg cgcccagcat gccgctgata tgcccgtaga
      481 accactcccg tttatcgtgc ttgcgtttgt agaaccgatg caactggatg accaccatgt
      541 agctgccgat ggcgccgaac acggtgaaca gcatggcttt ggtatcccgc tgccccatca
      601 gcaaatgggc caggccccag atgaggagac caccgttcac ggtgccagct atgccgtgca
      661 accagaggtc cgtaggctga ggcttcaggc cctcgtgcag ctttttcagg tagagcgacc
      721 ggtaaccgct ggccaccagg tggaaactga acacgcccac gagcgccagc cagaaactgc
      781 gctgatagtg ggcaatgccc agcacaacag cggtgataca gaccacgacc atgctgtaga
      841 agtaggcttt gccccagcgc ctgtgccaag tgccgccctt gggcaaggcc attgccagtg
      901 gggccaccac cagcgcgatg ccgcccgcta tggcatgcac acgaagtatc cacggaacaa
      961 ggtacccatc atccatgcgc gcgaacatag gggaatgcgc cttttcggat ccatgcatgt
     1021 ggtcaaacga ccgttgaagc atgacgaagc ggatcctggg cctggcacac gtgcgttgtc
     1081 aggggcgatc gggtagcgcg ctgcccgggc tattaccgtg tcatcgcggt gcgatccgga
     1141 cgtaaggcga cgtactccga tcaaaaatgg cgttcccgtt cgcatccaca tcccaagcgc
     1201 ggtcgagcag ccagagg
//
LOCUS       DAOQOC010001126         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106343_length_1217_cov_2.703959, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001126 DAOQOC010000000
VERSION     DAOQOC010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106343_length_1217_cov_2.703959"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PL106_14955"
     CDS             <1..873
                     /locus_tag="PL106_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34425.1"
                     /translation="GLPTTVRFDDPDAIWHVADTRPQGGPQDPSFIATTTTRYFYAGD
                     TTIAGETWRRMWAQATTDPPPTALFEGLVRQVNDLVLFMDTLATLDTLYDFGIQIGDS
                     VRYTMQGLADLYLNVVSLDSLLIQDVYHKVIQFDTSDALQTLESFVSGRWIEGVGSIH
                     GPLAPIRVEDLEDWYFMDSTRTICYRQDGPVLWQHPDFPECTVNILLGTAATAKPEVR
                     ILPNPASHLIRVQGLPRGNWAWRVIDARGATELEGRTSGTDPCAIDVRSLTTGIHVLW
                     ISAPVPIHLRFVKE"
     gene            complement(881..>1217)
                     /locus_tag="PL106_14960"
     CDS             complement(881..>1217)
                     /locus_tag="PL106_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNA34426.1"
                     /translation="KAAYIGASARQMQAVFNYEQTVLNAYVDVVNQLSNIQNLHHSYD
                     LRKQQVDALSSSITISNNLFRSARADYMEVLLTQRDALESKFELVETQKQRMNAMVNM
                     YQELGGGWR"
BASE COUNT          226 a          382 c          356 g          253 t
ORIGIN      
        1 ggcttgccga ccaccgtgcg cttcgatgat ccggatgcca tttggcacgt ggccgatacc
       61 cgaccgcaag gcggtccgca ggacccgtcg ttcatcgcca caacgaccac acggtatttt
      121 tatgcgggcg atacgacgat cgccggagag acctggcgcc gcatgtgggc gcaggccacc
      181 acggacccgc cgccaacagc acttttcgaa ggacttgtca ggcaggtgaa cgacctcgtc
      241 ctgttcatgg ataccctggc cacgctggat accctctacg acttcggcat ccagatcggg
      301 gactccgtcc ggtataccat gcagggcttg gctgatctct acctcaatgt ggtttccctg
      361 gacagcctgt tgatccagga tgtgtaccac aaggtgatcc aattcgatac cagcgatgcg
      421 ttgcagacac tggagagttt cgtctcgggc cgatggatcg agggcgtggg cagcatccac
      481 ggaccgttgg cccccatccg ggtcgaggat ctggaggact ggtatttcat ggactccacg
      541 cgtaccatct gctaccgcca ggatggacct gtgctctggc aacaccccga cttccccgaa
      601 tgcaccgtga acatcctgct cgggacagcg gccacggcga agccggaggt gcggatcctc
      661 ccgaaccccg cttcgcacct gatccgggtg caaggcctgc cacgcggcaa ctgggcctgg
      721 cgtgtgatcg atgcgcgcgg tgcaacggag cttgaaggcc ggacctcagg tactgacccc
      781 tgcgcgatcg atgtgcgctc cctgacaaca ggtattcatg tcctgtggat atcggcaccc
      841 gtgcccatcc acctgcgctt cgtcaaggaa taacgccgac ctaccgccag cctccaccca
      901 gctcctggta catattcacc atggcgttca tgcgctgttt ctgggtctcc accagctcga
      961 acttcgattc cagcgcatcg cgttgtgtga gcagtacttc catgtagtcg gcgcgggccg
     1021 agcggaacag gttgttggag atggtgatgg aactggagag cgcatccacc tgttgcttgc
     1081 gcaggtcgta gctgtgatgc agattctgga tgttgctcaa ctggttcacc acgtcgacat
     1141 acgcgttcaa gacggtctgt tcgtagttga atacggcctg catctggcgc gcgctcgcac
     1201 cgatgtacgc cgccttg
//
LOCUS       DAOQOC010001127         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106471_length_1216_cov_3.102498, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001127 DAOQOC010000000
VERSION     DAOQOC010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106471_length_1216_cov_3.102498"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1216
                     /locus_tag="PL106_14965"
     CDS             <1..>1216
                     /locus_tag="PL106_14965"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA34427.1"
                     /translation="HWDKSTGAITRTHHLPGLPDTKVGDMMYMGMDRQGVLWGCSWDH
                     GGFRFDPRDGTSSWFHVHDNRPGALPFGYIRDALDMPDGTIWLANLQGIAIHDPDAPQ
                     VRVIDANKGIELGNERSLGIGTIAVAGDSVLWAATDPPSFVRIDTRSGKVRRGSIENE
                     AGDRLWIRDPLVLRDGVMVNTTDGIWRIDDRLIRATRVELRDDSGHVLPHRLHSWLTT
                     DAAGLVWSAFGDQVYRFDPRTNTARTFSKGTGHSGWHGDGALSCGLGTSDGRFWVGIG
                     SAGLAVYDPQRDSWEEFSADVDAGRLRSTWITAMAESPDGSLWLATNASGLVKYDPRS
                     RVYTTYDQRHGFRAISLTSVQFDRMGRVWVGSSEGAMCFDPRTERAVRVDARSDQPFD
                     DLSFGSCTSESGV"
BASE COUNT          221 a          358 c          394 g          243 t
ORIGIN      
        1 tgcattggga caagtccacc ggagcgatca cgcgcacgca tcacctgcct ggccttcccg
       61 acaccaaagt gggcgacatg atgtacatgg gcatggaccg ccaaggtgtg ctttggggtt
      121 gttcatggga ccatggcggc ttccgcttcg acccgcgtga tgggacctcc tcctggttcc
      181 atgtgcacga caaccgacca ggcgcgcttc cgttcggtta catccgcgat gcgctcgaca
      241 tgcccgatgg caccatatgg ctggccaacc ttcagggcat tgccatacac gatcctgacg
      301 cccctcaagt gcgcgtgatc gatgcgaaca aagggatcga attgggcaac gagcgctcgc
      361 tgggcatcgg caccatcgcg gtggcgggtg atagtgtgct gtgggccgcc acggatccgc
      421 cctcgttcgt acggatcgac acgcgctccg gaaaagtgcg acgtggaagc atcgagaacg
      481 aagccggcga tcggctctgg atacgtgatc cgttggtgct gcgtgatggc gttatggtga
      541 acaccacgga cgggatctgg cggatcgatg atcgcctgat acgcgcaacg cgtgtggaat
      601 tgcgcgacga tagtggccat gtgcttccac atcgtctgca ctcctggctg accaccgatg
      661 ccgcaggact ggtgtggtcc gccttcggcg atcaggtgta ccgtttcgat ccgcgaacga
      721 acaccgcgcg cacgttcagc aaaggcaccg gccattccgg ttggcatggg gatggtgcgc
      781 tctcgtgcgg tctgggaaca tcggacgggc gcttctgggt tgggatcgga tcagcagggc
      841 ttgcggtcta cgatccacag cgcgatagct gggaagagtt cagcgccgat gtggatgccg
      901 gtcgattacg ttccacctgg atcaccgcca tggccgaaag cccggatggt agtttgtggt
      961 tggccaccaa cgcatcgggc ctggtgaagt acgatccgcg ctcgcgcgtg tacactactt
     1021 acgatcagcg gcacggcttc cgagcgatca gcctcacctc cgtgcagttc gatcgcatgg
     1081 gtcgtgtgtg ggtgggatcc tccgaagggg ccatgtgttt cgatccacgc accgaacgcg
     1141 ctgttcgcgt ggacgcgcgt tcggaccaac cgttcgacga cctgagcttc ggatcatgca
     1201 cgtcggaaag cggcgt
//
LOCUS       DAOQOC010001128         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106487_length_1216_cov_2.927649, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001128 DAOQOC010000000
VERSION     DAOQOC010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106487_length_1216_cov_2.927649"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..>1216)
                     /locus_tag="PL106_14970"
     CDS             complement(21..>1216)
                     /locus_tag="PL106_14970"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNA34428.1"
                     /translation="SRGPKKSFGNRGPRRDGGPGEGPRGPRGPRKEGDGPWRERSERP
                     RGGSGPRRDGPPRDDRPPRDRDRPAKRHGSDDRGERPAKPYRGSGERPAKPRAEKPWK
                     RQSRDERTFKEGDPLTRRPFKKGERSNRFSLPNPANEGLIRLNKYLATAGVGSRREAD
                     ELITTGAVTVNGKVVTELGTKIKPTDTVHFGGQKLSTEQKRYVLLNKPKDTITTTDDP
                     RERHTVMDLIAKAATERLYPVGRLDRNTTGVLLLTNDGDLAKRLTHPSHGAEKIYHVT
                     LDKAVHAAHLKELVEGVHLDDGPAHADEVSYVSAAATRREVGIKIHMGRNRIVRRMFE
                     ALGYEVVKLDRVMFAGLTKKDLQRGHWRHLSEKEVTWLKQKKAPAATEGDKPRGRLRR
                     SHGHE"
BASE COUNT          140 a          397 c          406 g          273 t
ORIGIN      
        1 cgttcgtggt gcagtgatcc ctactcgtgc ccgtggctgc ggcgcagccg tccgcgcggc
       61 ttgtcgcctt ccgtcgctgc cggggccttt ttctgcttca gccaggtgac ctccttttcg
      121 gacaggtggc gccaatgccc acgctgcagg tccttcttgg tcaggccggc gaacatcacc
      181 cggtccagct tcaccacttc gtagcccagg gcctcgaaca tgcggcgcac gatgcggttg
      241 cggcccatgt ggatcttgat gcccacttcg cggcgcgtgg cggcggcgct cacatagctc
      301 acctcgtcgg catgtgcggg gccgtcgtcc agatgaacac cttccaccag ttccttcagg
      361 tgcgccgcat gcacggcctt gtccagcgtg acgtggtata ttttttccgc gccgtggctc
      421 gggtgggtga gacgcttggc caggtcgcca tcgttggtga gcagcagcac gccggtggtg
      481 ttgcggtcca ggcggcccac ggggtacagg cgctcggtgg cggccttggc gatcaggtcc
      541 atcacggtat ggcgttcgcg cggatcgtcg gtggtggtga tggtgtcctt gggcttgttg
      601 agcagtacgt accgtttctg ttcggtactg agtttctgcc cgccgaaatg gaccgtgtcc
      661 gtgggtttga tcttggtgcc cagctcggtc accaccttgc cgttcaccgt gaccgctccg
      721 gtggtgatga gttcatcggc ctcacggcgg ctgcccaccc cggctgtcgc caggtacttg
      781 ttcaagcgga tcaatccctc gttggcggga ttgggcaggc tgaagcggtt gctgcgttcg
      841 ccttttttga aaggcctacg ggtcagcggg tcgccctcct tgaaggtgcg ctcgtcccgg
      901 ctctggcgct tccagggctt ttcggcgcgc ggcttcgcag gccgttcgcc acttcccctg
      961 tatggctttg cgggtcgttc gccgcggtca tccgacccgt ggcgtttggc gggacggtcg
     1021 cgatcgcgcg gtggtcgatc atcgcgcggc gggccatcac gacgcgggcc gctgccccca
     1081 cgaggtcttt cggagcgttc acgccaaggg ccatcgcctt ccttgcgcgg gccacgcggc
     1141 ccacggggcc cctcgccggg tccaccatca cggcgcgggc cacgattgcc gaagcttttt
     1201 ttcggtccgc gggaac
//
LOCUS       DAOQOC010001129         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106666_length_1215_cov_2.950862, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001129 DAOQOC010000000
VERSION     DAOQOC010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106666_length_1215_cov_2.950862"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PL106_14975"
     CDS             complement(<1..515)
                     /locus_tag="PL106_14975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34429.1"
                     /translation="MSAQAEPDPTTLRVLPWLVAVGLFMEHLDISILNTAIPVIAEDM
                     GITALSLKAVLTSYTLALAIFIPVSGWVADRYGTRRVFRTAVAVFTIGSLLCGLSTNL
                     PMLIASRFIQGVGGSMMVPVGRIALIRAYPRSRMLAMMNYVVIPALIAPLVGPFIGGS
                     IVHLFPWRTIFL"
     gene            complement(868..>1215)
                     /locus_tag="PL106_14980"
     CDS             complement(868..>1215)
                     /locus_tag="PL106_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34430.1"
                     /translation="KTPDIEYRNGVFHIVWSEGAAQQVRYRTATIQETTGMVSHGTPR
                     VSVHPDPAMDRITISGGPWTETTVLDAQGRPAGTLAPSGQCLQVASLAPGPYLLLLRA
                     VDGRMAFARFVKQ"
BASE COUNT          234 a          358 c          413 g          210 t
ORIGIN      
        1 agaaagatgg tgcgccaggg gaagaggtgg acgatggagc cgccgatgaa cggacccacc
       61 agcggggcga tcagcgcggg gatcaccacg tagttcatca tggccagcat gcgcgatcgc
      121 gggtaggcac ggatcaacgc gatgcggccc acgggcacca tcatgctgcc gcccacgccc
      181 tgtatgaagc gcgaggcgat gagcatgggc aggttggtgc tcagtccgca caacaatgaa
      241 ccgatcgtga acaccgccac cgccgttcgg aacacgcggc gcgtgccgta ccgatcggcc
      301 acccagccgc tcaccggaat gaagatggcc agcgccaggg tgtaactggt gagcaccgcc
      361 ttcaagctca atgccgtgat gcccatgtcc tcggcgatca cggggatggc cgtgttcagg
      421 atgctgatgt ccaggtgctc catgaagagc cccaccgcca ccagccaggg caatacccgg
      481 agggtggtag gatccggttc ggcttgtgcg gacacggtgc gaaggtcgta cgtctttcga
      541 tggtggcgcg atatatgggg tcgttaacgg ctttccgcgc gccgtccgga tgagctacgt
      601 tcctgatgga cggtcacggg cagggaaggg ctggtcggcc catgcggagg ggttatccag
      661 gtctgcacgg tacaactccg atgaccgggg aacagagcgc aggagcgtcc caaggcctcc
      721 gggtggacat gcggaacgtg cgcaacaagt tcgctgtgga tggcgatgcg tacactatgg
      781 acaacggtag ggagggagga ccggtccagc ggtgcggagc acttccacca ccaagatgtg
      841 ctccggagta gaggctcccg cagggactca ctgcttcacg aagcgggcaa aggccattct
      901 gccgtccacc gcgcgcaaca acagcagata cggaccaggt gccagggacg ctacttggag
      961 gcattggccg gaaggtgcca gtgtacctgc cggtcgtccc tgcgcatcaa gcacggtggt
     1021 ttcggtccag ggtcctccgc tgatggtgat acggtccatt gctgggtcgg gatgaaccga
     1081 tacccggggc gtcccgtggc tgaccatgcc ggtggtctct tggatggtgg ccgtgcggta
     1141 ccgcacctgc tgtgccgcgc cctcgctcca tacgatgtgg aacaccccgt tcctgtactc
     1201 gatatccggt gtttt
//
LOCUS       DAOQOC010001130         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_106690_length_1215_cov_2.672414, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001130 DAOQOC010000000
VERSION     DAOQOC010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106690_length_1215_cov_2.672414"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="PL106_14985"
     CDS             complement(<1..>1215)
                     /locus_tag="PL106_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34431.1"
                     /translation="CVDQPVYYNFGVNEPDGNTLVYSLISGQYFNGTNEQPLVYQPGF
                     SGAFPVPGITVDPITGQIAFTPSAIGNYVVVMEVAEYDGLGNLIGTVMRDITFVVIAC
                     TGNVPQTQGVVNNTAGILTSPSSIEVCDGQAFCVDVQFTDADPGTILQVVSQATALLP
                     GATFNVLGTNPATAHICWTGDVNYSPVNVLVQADDGNCPIENTASLAINIISVAGGGP
                     PPSPGTNGSVQICPGANAFFLVSVLGGTPDVNGFWTGPDGAAHNSEFNPLLDPPGVYT
                     YTTGNACAFATATATISFAAAPDAGTDGTLTVCSDAAPSDLFSSLGGTPSMSGSWTMP
                     GGASFSGTYDPTVNTPGAYVYTVVGGGGCPGASATVTVTEITAPEAGTDGAATFCTNG
                     GAQNLIGFLGGTP"
BASE COUNT          230 a          384 c          375 g          226 t
ORIGIN      
        1 ggcgttccgc ccaggaagcc gatcaggttc tgtgctcctc cattcgtgca gaaggtggcc
       61 gcaccatcgg taccggcctc cggcgccgtg atctcggtca cggtgaccgt ggcgctcgcc
      121 ccaggacaac ctcctccgcc gacgaccgtg tacacgtagg cacccggggt attcaccgtg
      181 ggatcgtaag taccactgaa tgatgcacca ccaggcatgg tccaggagcc ggacatcgaa
      241 ggcgttcccc ccaggctgct gaacaggtcg ctcggcgccg catcggaaca caccgtcaac
      301 gtcccgtccg tgccggcatc gggtgctgcg gcgaacgaga tggtggccgt ggccgtggcg
      361 aaggcgcacg cgttccccgt ggtataggtg tacacaccgg gcggatccag caagggattg
      421 aactcggaat tgtgcgctgc tccgtcgggt ccggtccaga agccattgac atcaggggtg
      481 ccgcccagaa cgctcaccag gaaaaaggca tttgcgcccg gacagatctg taccgagccg
      541 ttcgttcccg gcgatggcgg cgggccgcca cccgccacgg aaatgatgtt gatggcgagt
      601 gatgcggtat tctcgatcgg gcagttccca tcatcggcct gtaccagcac gttcaccggt
      661 gaatagttca catccccggt ccagcatata tgcgccgtgg cagggttggt gcccagcaca
      721 ttgaatgtgg cgcccggaag cagggccgtg gcctgggaca ccacttgcaa tatggtaccg
      781 gggtccgcat ccgtgaattg aacgtccaca cagaaggcct gcccgtcgca tacctcgata
      841 ctgctcgggc tcgtgaggat gcccgccgtg ttgttcacaa caccttgcgt ttggggcaca
      901 ttgccggtac acgcgatgac cacgaacgtg atgtcccgca tcaccgtgcc gatcaggttc
      961 cccaaaccgt cgtactcggc cacctccatc accaccacgt agttgccgat ggccgagggg
     1021 gtgaaagcga tctgccccgt gatggggtcc accgtgatcc cgggtacggg aaaggctcct
     1081 gagaagcccg gttggtacac gagcggttgc tcgttggtcc cgttgaaata ctgaccggag
     1141 atcagtgagt acaccagtgt attgccgtcc ggctcattca ccccgaagtt gtagtacacc
     1201 ggctggtcca cgcag
//
LOCUS       DAOQOC010001131         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_107017_length_1213_cov_3.454231, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001131 DAOQOC010000000
VERSION     DAOQOC010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107017_length_1213_cov_3.454231"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..>1213)
                     /gene="atpB"
                     /locus_tag="PL106_14990"
     CDS             complement(58..>1213)
                     /gene="atpB"
                     /locus_tag="PL106_14990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNA34432.1"
                     /translation="SFPKSLRAALVLASALFVSTGRGQHQGVQEPAGNEDAVAEEVLH
                     DVSGDDLEVESQPNGSFKAGELIMEHIGDSHDWHLWGHTHLPLPIILKVGDGWNWELF
                     SSSRFMDEHHHPASYRGKHGEYRLVGKNIVGTCGVDCVLKVTDLSITKNVATLFFCVA
                     LMLLVFVGIARGYRKRGVAAPQGPASLLEPIILFVRDDIAKSSIGEKHYQRFTPYLLT
                     LFFFIWFLNLIGLIPIFPFGANLTGNIVTPLVLAAATFLIVTFVANSHYWRHIVAMPG
                     IPPFVLIILTPIEIMGHFIRPIVLVIRLFANIMAGHIVLLVFFCLIFIFSKNGANPVG
                     GWATSPFVLAFTVFINCLELLVGALQAYVFTLLTSIYIGTAVAGGHDDHH"
BASE COUNT          261 a          328 c          402 g          222 t
ORIGIN      
        1 ggaggatgtt caaagtggtc atcgaagtgg gaagttgaag gttcgggggt tgttgtctta
       61 atgatggtcg tcgtgcccac ccgccacagc cgtgccgata taaatgctgg tgagcagggt
      121 gaagacgtat gcctgcaggg cccccaccag gagctccaag cagttgatga ataccgtgaa
      181 ggccagcacg aaggggctgg tggcccagcc gcccacgggg ttcgcaccgt tcttgctgaa
      241 gatgaagatc aagcagaaga acaccagcag cacgatgtgg ccggccatga tgttggcgaa
      301 gagtcggatc acgagaacga tggggcggat gaagtgcccc atgatctcga tcggggtgag
      361 gatgatgagc acgaatggcg ggatgccggg catcgccacg atgtggcgcc aatagtggct
      421 gttcgctacg aaggtgacga tcaggaaggt ggctgccgcc agtaccagcg gggtaacgat
      481 gttgcccgtg aggttggcac cgaaggggaa aatggggatg agaccgatga ggttgaggaa
      541 ccagatgaag aagaacaggg tgagcaggta cggtgtgaag cgctggtagt gcttctcgcc
      601 gatggagctc ttggcaatat catcccgaac aaagagtata atgggctcca gcaggctggc
      661 gggtccctgc ggggcggcca caccgcgctt gcggtacccg cgtgcgatac cgacgaaaac
      721 caggagcatc aacgccacgc agaagaagag cgtggccacg ttcttcgtga tgctcaggtc
      781 ggtgaccttg agtacacagt cgacgccgca ggtccccacg atattcttac ctactaatcg
      841 gtattcgcca tgcttccccc ggtacgatgc ggggtggtgg tgctcatcca tgaagcggct
      901 gctcgagaaa agctcccagt tccacccatc gcccaccttc aggatgatgg gcaggggcag
      961 gtgggtgtgc ccccagaggt gccagtcgtg gctgtcgccg atgtgctcca tgatgagctc
     1021 accggccttg aatgatccgt tcggctggct ctccacctcc aggtcgtcac ccgaaacgtc
     1081 gtgaaggacc tcctcggcga ccgcatcctc gttcccggca ggctcctgca caccttgatg
     1141 ctgcccacgg cctgtgctca cgaacagcgc ggatgcgagc accagcgcgg cgcggagcga
     1201 ttttgggaac gac
//
LOCUS       DAOQOC010001132         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_107024_length_1213_cov_3.297064, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001132 DAOQOC010000000
VERSION     DAOQOC010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107024_length_1213_cov_3.297064"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PL106_14995"
     CDS             complement(<1..1049)
                     /locus_tag="PL106_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34433.1"
                     /translation="MNLRRTALFILLLAAQAHGQDRALDSLRSALDTTRQDTTQLRTL
                     VRFSQIASLSSPDTVYARFMARLIGKLEQDPAASVRAVAEEAKFNWLIWKGQMRRRVS
                     DYRGAVRMFEEAGRLAEARADTARLLDYEMELGDLYGRINDTITGLRHGYRAMEYAIA
                     LGDSALAARILFGVARFDTSRVRLLSSLEYVDAYAKKARWENNAGYYAARGWMLQAHG
                     EHDQAIANLERADSLLSDPDSRMIALYAWFLGEAYLSVGRSDDAIASFRRALSAAERH
                     GINGWHAGCALALGSELKRTGDQAGAEAAWLDALAHARLGNFPDHKLTALGHLKDLYT
                     DQHRYREALETTAQWL"
BASE COUNT          224 a          372 c          378 g          239 t
ORIGIN      
        1 agccattgtg ccgtggtctc caaagcctct cgatagcggt gttgatccgt gtacaggtct
       61 ttcaagtggc ccagcgcggt gagcttgtgg tccgggaaat tcccgaggcg cgcgtgcgcc
      121 aaggcatcca accatgccgc ttcggccccg gcttggtcac ctgttctctt cagttcggaa
      181 cccaatgcca aggcgcagcc tgcgtgccat ccattgatcc catggcgttc agcagcggag
      241 agcgcacgcc ggaacgatgc aatggcgtca tccgatcgac caacgctcag atatgcttcg
      301 ccaaggaacc aggcgtacag ggcgatcatc cggctatcgg gatccgacaa caggctatcg
      361 gcccgttcca ggttggcgat ggcctggtca tgttcaccgt gagcttgcag catccatcca
      421 cgagcggcgt agtagccagc gttgttctcc catcgggcct tcttcgcgta cgcatcgacg
      481 tattccagag atgaaaggag gcggacgcgt gaagtatcga agcgggccac accgaaaagg
      541 atccgcgccg ccagcgcgct atcgcccaac gcaatggcgt attccatcgc gcgatagcca
      601 tgtcgcaagc cggtgatcgt atcgttgatc cgcccgtaga ggtcacccag ttccatctcg
      661 tagtccagga ggcgagcggt gtcggcccgc gcttcggcca gtcgaccagc ctcttcgaac
      721 atgcgcacgg cgccgcgata gtccgataca cgccgccgca tctggccctt ccagatgagc
      781 caattgaact tcgcttcctc cgcgaccgcc cttaccgagg cagccggatc ctgttccagc
      841 ttgccgatca acctggccat gaaccgggcg tacaccgtgt cggggctgga gagggaggcg
      901 atctgcgaga acctcaccag ggtgcgaagc tgcgtggtgt cctgcctggt ggtgtcgagc
      961 gcgctcctga gggagtcgag cgcacggtcc tggccgtggg cttgcgcggc caagagcaat
     1021 atgaaaaggg ccgtgcgacg gaggttcatg taccagtaga tgtcggtgcg ctgtcggacg
     1081 ttcccgatga cctctgcacg aagtacatct ccatctcccc cttgcccttg gcctgcactt
     1141 ttccgcgcgg ggtgaatgcg aatgcgggtg aatgggagtt ggtgaatggt gaatgggccg
     1201 ttgcgggcga atg
//
LOCUS       DAOQOC010001133         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_108112_length_1207_cov_3.231771, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001133 DAOQOC010000000
VERSION     DAOQOC010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108112_length_1207_cov_3.231771"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1207)
                     /locus_tag="PL106_15000"
     CDS             complement(2..>1207)
                     /locus_tag="PL106_15000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNA34434.1"
                     /translation="LSIPVFFLLIGAELLWSAWTHRKLYTFPDLIANIGCGIGSQVVG
                     AFTKAAIFGVYLWTYDHLRVFTLPANVLIWVITFLLVDLGYYWFHRLSHEVNFLWAAH
                     IVHHQSEEYNLSVALRQSWWQGLFSFWFYLPIAVLGVHPVVILTVGALNTLYQFWIHT
                     RAIGRMGWLEHVFNTPSHHRVHHGSDPKYLDRNHAGSLIIWDKLFGTFQPEEEEPVYG
                     ITTPLRSWDPVWANFHYWADLVALARRCTALGDKVRVFLKPPGWRPADLGGRERPKEH
                     DRTNYLKYSTDAGANARRYVLVQFFLLLAVTSLFLFKQDSLPIWGAWVLAGIIVLWVM
                     GLGLVLEGRRGALTLEAVRVLLFTGAGIAVWMSVLGWSWWCAVIIFLGAASMIHLTAL
                     CGEGEVAGE"
BASE COUNT          254 a          401 c          359 g          193 t
ORIGIN      
        1 ctcactcccc ggccacctct ccttcaccgc acagggccgt aagatggatc atgctggccg
       61 cgcccaggaa gatgatgacc gcacaccacc acgaccagcc gagcacgctc atccacaccg
      121 cgatacccgc cccggtgaag agcagcacac gcaccgcctc cagcgtcaag gccccgcgcc
      181 gcccttcgag caccaacccc aggcccatca cccagagcac gatgatcccg gccagtaccc
      241 acgcgcccca gatgggcagg ctgtcctgct tgaagaggaa caaggatgtg acggccagca
      301 ggaggaaaaa ctgcaccagg acatagcgcc gcgcgttcgc acctgcatcc gtactgtact
      361 tgaggtagtt ggtgcgatcg tgctccttcg gtcgttcgcg accgcccaga tccgctggtc
      421 gccagccggg gggcttcagg aagacgcgca ccttgtcgcc cagggccgtg caacggcggg
      481 ccagcgcaac aaggtccgcc cagtagtgga agttggccca caccgggtcc cagctcctca
      541 acggggtggt gatgccgtat accggttcct cctcctcggg ctggaacgtt ccgaagagtt
      601 tgtcccagat gatgagcgag cccgcgtggt tgcggtccag gtatttcgga tcgctgccgt
      661 gatgcacccg atggtggctc ggcgtgttga agacatgctc cagccagccc atccgcccga
      721 tggccctggt gtggatccag aactggtaca gcgtattgag cgcgcccacc gtgaggatga
      781 ccacaggatg tacgcccagc accgcgatgg gcaggtagaa ccagaagctg aacagcccct
      841 gccaccagct ctgccgcaat gccacgctca aattgtactc ttcgctctgg tgatgtacga
      901 tgtgcgcggc ccacaggaag ttcacctcgt ggctcagccg atggaaccaa tagtagccca
      961 ggtccaccag caggaaggtg atcacccaga tcagcacatt ggccggcagg gtgaacacgc
     1021 gcagatggtc gtacgtccac aggtaaacac cgaagatggc cgccttggtg aaggctccta
     1081 ccacttgcga gccgatgccg cacccgatgt tggcgatcag atccgggaag gtgtacaact
     1141 tgcggtgcgt ccatgcgctc cagagcaact ccgcgccgat gagcaggaag aagaccggga
     1201 tcgagag
//
LOCUS       DAOQOC010001134         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_108174_length_1207_cov_2.513889, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001134 DAOQOC010000000
VERSION     DAOQOC010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108174_length_1207_cov_2.513889"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..381)
                     /locus_tag="PL106_15005"
     CDS             complement(82..381)
                     /locus_tag="PL106_15005"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA34435.1"
                     /translation="MELKFTQYAIAHLVETVELTFSGATMKKREEVMDRVLEATRRLL
                     EWPNAGQVETWMEGREHIYRRLVVGNFKVIYRVDGETIYITDIFDARQNPGRMKG"
     gene            complement(387..617)
                     /locus_tag="PL106_15010"
     CDS             complement(387..617)
                     /locus_tag="PL106_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34436.1"
                     /translation="MTAIQLKDHLIELIQKEGDLNLLKLLNKLLDRSTSESAFRVMLA
                     EGADRSEADIKAGAVLSLEQAKAEAKAALRKK"
     gene            complement(650..>1207)
                     /locus_tag="PL106_15015"
     CDS             complement(650..>1207)
                     /locus_tag="PL106_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNA34437.1"
                     /translation="SVVDKPIKFIGTGEKMEALDEFHPDRMAGRILGMGDVVSLVERA
                     QEQFDEKAARELNKKIAKDQFGFDDFLEQIGQIKKMGNMKDLMGMIPGVGKAIKNIDI
                     PDDAFKGIEAIIQSMTPVERKNPSVINGSRRTRLAKGSGRGIEEVNKLMKQFEEMRKM
                     MRMMGDKTKMQNMMRQMQQMQGMRR"
BASE COUNT          195 a          400 c          287 g          325 t
ORIGIN      
        1 atcatccgca tcatcttccg catctcctcg aactgcttca tcagtttgtt cacttcctcg
       61 atgcctctgc tcgagaggag atcatccctt catccttcca gggttctgtc gggcgtcaaa
      121 gatgtcagtg atgtagatcg tctcgccatc cacccggtag atcactttga aattgccgac
      181 cacaagacgg cggtatatat gctcgcgccc ttccatccat gtctccactt gtcctgcgtt
      241 cggccattcg agcagtcgcc gcgtcgcttc cagcaccctg tccatcacct cttcgcgttt
      301 cttcatagtc gccccactaa aggtcagttc cacggtctcc accaagtgcg cgatggcgta
      361 ttgggtgaac ttcagttcca ctgttgctac ttcttccgaa gtgccgcttt cgcctcggcc
      421 ttggcctgct caagggaaag taccgctccc gccttgatat ccgcttcgga gcgatcggct
      481 ccttcggcca gcatcacccg aaaagcgctc tcgctggtgg atcggtccag cagtttgttc
      541 agcaacttca gcaggttgag gtccccttcc ttctggatga gctcgatcaa gtgatccttc
      601 aattgtatgg cggtcattgg gcgttgctga tgaacaaagc tatggcgaat cacctcctca
      661 tcccttgcat ctgctgcatc tgccgcatca tgttctgcat cttggtcttg tcccccatca
      721 tccgcatcat cttccgcatc tcctcgaact gcttcatcag tttgttcacc tcctcgatgc
      781 cgcgaccact gcccttcgcg aggcgtgtgc ggcggctgcc gttgataact gacgggttct
      841 tgcgctccac gggtgtcatg ctctggatga tcgcctcgat acccttgaag gcgtcatcgg
      901 gtatgtcgat gttcttgatg gccttgccca cgccggggat catgcccatg aggtccttca
      961 tgttccccat tttcttgatc tgcccgatct gctccaggaa atcgtcgaag ccgaactggt
     1021 ccttcgcgat cttcttgttc agctcgcgtg cggccttctc gtcgaactgc tcctgcgcgc
     1081 gctccaccag cgagaccaca tcgcccatgc cgaggatgcg ccccgccatg cggtccgggt
     1141 ggaactcgtc cagcgcctcc attttctcgc ccgtgccgat gaacttgatc ggcttgtcca
     1201 ccacgct
//
LOCUS       DAOQOC010001135         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_108283_length_1206_cov_3.708949, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001135 DAOQOC010000000
VERSION     DAOQOC010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108283_length_1206_cov_3.708949"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..>1206)
                     /locus_tag="PL106_15020"
     CDS             complement(568..>1206)
                     /locus_tag="PL106_15020"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNA34438.1"
                     /translation="VAAINAEIDRLIRDKVVDFNFTGRKIMFAFHHSELLRKYVHDRR
                     ILDVMDFLLQKRVKVFQSINFLTGSEQHAHSDSIHMTTHPLGYMTAAWIALEPTTADN
                     GPLIYHVGSHELPYLLNGSYDHGGNRFTIGEDAYARYEEAVDRELASGTFEAREFHAR
                     PGDVLLWHANLIHGGKKMNTPDASRKSMVIHCFADDVLCYHELTQRAAMMEE"
BASE COUNT          227 a          336 c          383 g          260 t
ORIGIN      
        1 tgcaaaagcc gtcccgcgca gaaaggagga gatcgtggtt cccatcgtga ctcattcaat
       61 gcgccagcca ttctgcccgt cccaatcgat gccgacctcg gtttcgcaac aggttcctgt
      121 ggttgttcca ttcgcgcagt cccaatgggc tatgaggggg acgagaggcg ggttacttat
      181 gcaatcataa taaccgcagt acaggtccat tttctgaacg accgttacac cagctgggag
      241 tgtgagcgat acagttccgg gtccgttcag gggcacacag cctgtgtaca agggctctga
      301 ggatgcgccg caactaccac cggaggcgtc gtagacgata gtgaagtcac aatccaactc
      361 cgtcccgata gtggggtgag gtggtggtgg atcgttgttg gaggcggaga ggcagagcac
      421 cgcggcgaga aacagaacgt gccggagaga ggaggtgatc ttttttcatg gttgcgtggt
      481 tgtaataacg aaggtacgca tccgaacgat ccgatgtgag ggttgatttc tatctggctc
      541 gcttcgcgat caacgccatc cacagcctca ctcctccatc atcgccgccc gctgcgtcag
      601 ctcgtggtag cacagcacat catcggcgaa acagtggatc accatgctct tccgtgaagc
      661 atcgggcgtg ttcatcttct tgccgccgtg gatgaggttg gcgtgccaga ggagcacgtc
      721 gccgggccgc gcatggaact cacgtgcctc gaacgtgccg ctggccagct cgcggtccac
      781 ggcctcctcg tagcgtgcat aagcgtcctc gccgatcgtg aagcggttgc cgccatggtc
      841 ataactcccg ttgagcaggt aggggagttc gtggctgccc acgtggtaga tgagcggccc
      901 gttgtcggcg gtggtgggct ccagggcgat ccacgcggcg gtcatgtagc ccagcgggtg
      961 cgtggtcatg tggatgctgt cgctgtgcgc gtgctgttcg ctgccggtga ggaagttgat
     1021 gctctggaac accttcacgc gcttctggag caggaagtcc atcacgtcga ggatccgccg
     1081 gtcgtgcacg tacttccgga gcagttcgct gtggtggaag gcgaacatga tcttcctccc
     1141 ggtgaagttg aagtccacca ccttgtcgcg gatcagccgg tcgatctccg cattgatggc
     1201 cgctac
//
LOCUS       DAOQOC010001136         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_108385_length_1206_cov_2.015639, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001136 DAOQOC010000000
VERSION     DAOQOC010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108385_length_1206_cov_2.015639"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PL106_15025"
     CDS             <1..928
                     /locus_tag="PL106_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34439.1"
                     /translation="NSAALQFLAAHASGTTVQQSIGLGQYSWCLPRATLTLRKTSVGG
                     TGTFGFALTNTTQTTGTITTTAAGTPQQVDGDTSASGTQAYLVNTIGTAITINENSLP
                     SGWSLTGATCTNAAGSTVGSLSGSTYTIPAGNVTNGEAFTCTFTNTRSPTLQLAKAWG
                     PNSSAGDSATIGATTGGSANTSPFSTPGGTNANSGAAVNIQVGNTITLPAETGTNIGN
                     YNTVLSCTANGGATANALSGTNGQVSNTLLIGAGDAGKAIVCTYTNTRRSTTFRLAKA
                     WGANSVAGKVASLGATTGLTNNTALFTSTAIS"
BASE COUNT          262 a          357 c          375 g          212 t
ORIGIN      
        1 caacagcgcc gctcttcaat tccttgcagc gcatgcctcg ggaacgacgg ttcagcaatc
       61 cattggtctt ggccagtatt cgtggtgtct cccgcgtgcc acgttgaccc tgcgcaagac
      121 atccgtggga ggaaccggta cgttcggttt tgccttgaca aataccacac agaccacggg
      181 cactatcacc accacggccg ccggtacacc gcaacaggtg gatggcgaca cttcggcgag
      241 cggaacccag gcatatcttg taaacaccat tggtacggcg atcaccataa acgagaacag
      301 cctgccttcg ggctggagcc tgaccggggc gacctgtacc aatgccgccg ggtctaccgt
      361 gggaagtctg tccggctcaa cctataccat ccctgcaggc aatgtgacta acggcgaggc
      421 gtttacctgt accttcacca ataccagatc acccacgctg cagttggcga aggcctgggg
      481 cccgaacagt tcggccggtg acagcgccac gattggcgcg acaacgggcg gcagtgcaaa
      541 tacctctccg ttcagcactc cgggtggcac aaacgccaac agcggcgcgg cagtcaacat
      601 ccaggtcggc aatacgatca cgctcccggc ggaaacgggg acgaacatcg gcaactacaa
      661 taccgttttg agctgtacgg ccaacggcgg cgcgacagcg aacgccctgt cgggcacaaa
      721 cggtcaggtg tcgaatacgt tgctgatcgg ggccggggat gcggggaagg cgattgtctg
      781 tacctatacc aacacgcgcc ggagcaccac cttccggttg gcgaaggcat ggggcgcgaa
      841 cagcgtcgcc ggcaaggtgg cgagcctggg ggcgacgacg gggctgacga acaacacggc
      901 gctgttcacc agcacggcga tctcttgacc aagcagatcg tagatgcgga gcgacaccgg
      961 tccggacgac ggaatcgaga agcggatggt ggtggaggga ttgaacgggt tcggatagtt
     1021 ctgttccagc gtcatcgccg aggggacggc agcgaacgag cccagtgttg cattcgtgag
     1081 gatccgttcc ttcagtttct ggatgtgcgc atacgcggtg tcgatgcgcc acgcggcgat
     1141 cttcccttcg gagatcttcc tggtgacggt ggaaacgata taaccggtga gggaccaatt
     1201 cgcata
//
LOCUS       DAOQOC010001137         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_108660_length_1204_cov_3.436031, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001137 DAOQOC010000000
VERSION     DAOQOC010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108660_length_1204_cov_3.436031"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PL106_15030"
     CDS             complement(<1..797)
                     /locus_tag="PL106_15030"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNA34440.1"
                     /translation="MSNILLIGTGRMAHNLGHAIRKAGHTIISITGRDPMKLAAIGRY
                     LDAPTVRWDHAWPEADITVIATSDDTIGTVSARVPSTGRIVIHTAGTVDLDVLTPHEN
                     RGVLWPVQTLSHGAPTDLGDIPLIVDGSTPPALDAVLKLARSISGSVLQLSLEQRKRM
                     HLAAAITSNLPVFLVREAQRILRKENLPPDLLMPLWRNTAANVASIGPEHALTGPARR
                     GDVRSIRGHLALLEGEPDLRRAYALISTMIMKAYGHPADGLENVQGDT"
     gene            complement(939..>1204)
                     /locus_tag="PL106_15035"
     CDS             complement(939..>1204)
                     /locus_tag="PL106_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34441.1"
                     /translation="KTGSHLAKETTGEIGLVKRQTIYSGDVVNTVARIQSTCNVHGVD
                     NLVSKDLLDVLLPPAGSWSVRPIGEVPLKGKRNAVMLYTVEPK"
BASE COUNT          185 a          368 c          391 g          260 t
ORIGIN      
        1 gtatctcctt gtacgttctc gaggccatcg gcagggtggc cgtacgcctt catgatcatg
       61 gtgctgatca gcgcgtaggc gcggcgaagg tcgggttcgc cctccaataa cgcgagatga
      121 ccgcggatgg aacgtacatc gccacgccgg gcggggccgg tgagcgcatg ctccggcccg
      181 atgctggcca cgttggcggc cgtgttgcgc cacagcggca tcaacaggtc cggtggcagg
      241 ttctccttgc gcaggatgcg ctgggcctcg cgcaccagga acacgggcag gttgctggtg
      301 atcgcggcgg ccaggtgcat ccgtttccgt tgttccagcg aaagctgaag cacgctgcca
      361 ctgatggacc gggccagttt gagcaccgcg tccagtgctg gtggcgtact cccgtccacg
      421 atgagcggta tatcgcccag gtccgtaggt gcgccatggc tcagggtctg caccggccag
      481 agcactcccc ggttctcatg cggggtcagt acgtcgaggt cgaccgtgcc tgcggtgtgg
      541 atcacgatcc ttccggtgga cggtacgcgt gcgctcaccg tgcctatggt gtcgtcgctc
      601 gtggcgatca ccgtgatgtc cgcctcgggc catgcgtggt cccatcgtac ggtgggcgcg
      661 tccagatatc gtccgatcgc cgccagcttc atggggtcgc ggccagtgat gctgatgatc
      721 gtatgcccgg ccttgcggat cgcgtggccg aggttgtggg ccatgcggcc cgttccgatg
      781 aggaggatgt tcgacatggt cccgtcagtg cggcatgttc aattggtggg cggcaaagat
      841 cgcgacaggg ccgcgatacg atctgaaccg gtccatcttg ggcgtgcggt ccgtcggtcc
      901 ggactggaca ggaggaccca ttcgtccctc cgcgttcgct acttcggttc cacggtgtac
      961 agcatcaccg cgttccgttt gcctttcaag ggcacttcgc cgatcgggcg aacgctccat
     1021 gaacccgctg gcggtaacaa cacgtccagc agatccttgc tgaccagatt gtccaccccg
     1081 tggacattgc acgttgattg gatgcgtgcg accgtgttca ccacatcgcc actgtatatg
     1141 gtctggcgtt tcaccagacc gatctctccc gtcgtttctt ttgcaagatg gctaccagtt
     1201 ttgt
//
LOCUS       DAOQOC010001138         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_108704_length_1204_cov_2.724978, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001138 DAOQOC010000000
VERSION     DAOQOC010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108704_length_1204_cov_2.724978"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PL106_15040"
     CDS             complement(<1..221)
                     /locus_tag="PL106_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA34442.1"
                     /translation="MKKVLVTGGAGFIGSHTCVELAAAGFEPIIVDDLRNSEERAIDG
                     IARIVGRKPAVHRIDCCDEAAMERMFQGEG"
     gene            492..>1204
                     /locus_tag="PL106_15045"
     CDS             492..>1204
                     /locus_tag="PL106_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34443.1"
                     /translation="MSHHRPTPSTLAIAAVMAFFALITWRWSWNQGPPIIRSDAEGYY
                     GYLRAVFINHDLGHERLNSTYLHGTPDGTLNKYFAGEAVMLTPFFLAAHGWAHLSGDV
                     PDGLSTPYLRAVGLAGLTYALLGLLAIRAFLRRSGIAEAAIAAVLLAIGFGTQLVQYA
                     SIQPGWSHVYSFCAVSVFLLLTERLSRSMSIRRCVFWGLSLGLVVLLRPVNGLVVLAL
                     PVALGTGAWPFLRTFFGRR"
BASE COUNT          182 a          390 c          375 g          257 t
ORIGIN      
        1 ccctccccct ggaacatgcg ctccatggcg gcctcgtcgc agcagtcgat gcgatgaacg
       61 gcaggcttcc ggcccacgat gcgtgcgatg ccatcaatgg cgcgctcctc gctgttgcgc
      121 aggtcgtcca caatgatggg ctcgaagccc gcagcggcca gttccacgca ggtatgcgat
      181 ccgatgaatc cggcaccacc ggttacgaga accttcttca ttgtggcggt gaaattggcg
      241 agaaaggggc tgatagcatc tcgtaataat gcatagcccg acctgattgc cctttgttcc
      301 cactacttca tcgaggctcg cgttgcgtag ctcgggtcgt gctacgccat agctggctcc
      361 gtgcttcgcg cgccgacgcg ctacgcaacg cgatccccgg ctcgtcctgc gaaaaattgg
      421 cggcgtatcc cttggaccat cccatactgc gaaatggtat aacgtcgtcc ctcttcgcac
      481 attcgcgcca gatgtcccat cacaggccga ccccgtccac gctggccatt gccgcggtga
      541 tggccttctt cgcactcatc acatggcgtt ggagttggaa ccagggcccg ccgatcatcc
      601 gcagcgatgc ggagggctat tacgggtact tgcgggcggt gttcatcaac catgacctcg
      661 ggcacgaacg cctgaacagc acgtacctgc acgggacccc ggatggcacg ctgaacaagt
      721 acttcgctgg ggaggcggtc atgctgaccc ctttcttcct cgcggcgcac gggtgggccc
      781 acctgtcggg tgatgtcccg gacggactgt cgaccccgta cctgcgcgcc gtcggtctgg
      841 cggggctgac ctatgcgctg ctcggcctgc tcgccatccg ggcgtttctc cggagatcgg
      901 gaattgcgga ggcggcgatc gccgccgtgc tgctcgccat cggctttggc acgcaactcg
      961 tccagtacgc gagcatccaa cccggttggt cgcacgtgta ctcgttctgc gcggtgagcg
     1021 tgttcctgct gctcaccgag cgactctccc gttccatgag catccggcga tgcgtgttct
     1081 gggggttgtc cttggggttg gtggtgctcc tgcgcccggt gaacggtctg gtggtgctgg
     1141 ccttgccggt ggcactggga acaggtgcgt ggccgttcct gcgcacgttt ttcggccgtc
     1201 ggtg
//
LOCUS       DAOQOC010001139         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_109279_length_1201_cov_3.627400, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001139 DAOQOC010000000
VERSION     DAOQOC010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109279_length_1201_cov_3.627400"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PL106_15050"
     CDS             <1..602
                     /locus_tag="PL106_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNA34444.1"
                     /translation="NLYLIPTAEVPITNLYRDVTVEEGRLPIRNVGYTPCFRREAGSY
                     GAHVRGLNRVHQFDKVEIVRIEKPENSYAALEGMVEHVKGLLRDLELPYRQLLLCGGD
                     MGFASAMTYDMEVYSAAQQRWLEVSSISCFETYQSNRLKLKFKEDKKTRLLHTLNGSA
                     LALARIVAALLENNQTPDGIRIPDALRSYTGFDMIRKQP"
     gene            599..>1201
                     /locus_tag="PL106_15055"
     CDS             599..>1201
                     /locus_tag="PL106_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34445.1"
                     /translation="MSRTGTKNIPVRGYEGASVRLFCPRTLAPSHPRTLALRLPQFLF
                     VVLALLSVPSFAQGTDQQLAEQYFDQGDFERAALYFDKLYKERPSPITYEYLFKCRTA
                     LGQHEEAAKLAKEQYRRGNDPVFLVDLGQATRLMGDTAKAVEHFNKAMKGLKDDVNSV
                     RTLARAFEKAGEPDRALSVYERGKRTIKDGGWSFDYEIAQL"
BASE COUNT          251 a          395 c          362 g          193 t
ORIGIN      
        1 acaacctcta tttgatcccc acggccgaag tgcccatcac caacctgtac cgcgacgtca
       61 ccgtcgagga agggcgcctg cccatcagga acgtggggta tacgccctgt ttccgccgcg
      121 aggccggcag ctacggtgcg catgtgcgcg gtctcaaccg cgtgcatcag ttcgacaagg
      181 tggagatcgt gcgcatcgag aaaccggaga acagttatgc ggcgctggaa ggcatggtgg
      241 agcacgtgaa gggcctgcta cgcgacctgg aactccccta ccgccagttg ctgctatgcg
      301 gcggcgacat gggcttcgcc agcgccatga cctacgacat ggaggtgtac agcgcagcgc
      361 aacagcgctg gttggaggtg agttccatca gttgcttcga gacctaccag agcaaccgcc
      421 tgaagctgaa gttcaaggag gacaagaaga cccgcctgct gcacacgctc aatggcagcg
      481 cactcgcttt ggcacggatc gtggcggccc tgctggagaa caaccagacg cccgacggca
      541 tccgaattcc cgatgccctg cgctcgtaca cgggcttcga tatgatccgc aagcagccat
      601 gagcagaacg gggacgaaaa acatcccggt gcgagggtac gagggtgcga gcgtgcgact
      661 cttctgccct cgcacgctcg caccctcgca ccctcgcacc ctcgcactcc gcctccccca
      721 attcctcttc gtcgtcctcg ccctcctctc cgtcccgtcc ttcgcgcagg ggaccgacca
      781 acaactcgcc gaacagtatt tcgaccaggg ggatttcgag cgtgcggccc tctacttcga
      841 caagctgtac aaggagcggc catcccccat cacgtacgag tatcttttca agtgcaggac
      901 cgcgctgggg cagcacgagg aggccgccaa actcgccaag gaacagtacc gccgcggcaa
      961 cgacccggtc ttcctggtgg acctgggcca ggcgaccaga ctgatgggcg ataccgccaa
     1021 ggccgtggaa cacttcaaca aggcgatgaa gggcctgaag gacgacgtga acagcgtgcg
     1081 caccctggcc cgcgccttcg agaaggccgg tgagccggac cgggccctca gcgtgtatga
     1141 gcgcggcaaa cgcacgatca aggacggtgg ctggtcgttc gactacgaga tagcccagct
     1201 g
//
LOCUS       DAOQOC010001140         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_109720_length_1199_cov_2.931818, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001140 DAOQOC010000000
VERSION     DAOQOC010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109720_length_1199_cov_2.931818"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PL106_15060"
     CDS             complement(<1..408)
                     /locus_tag="PL106_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34446.1"
                     /translation="MFTVQELDELIDAGLDATGSQRYLFDQDKKPAINSAISRAITAG
                     GWALANRKGSEEMLWELKEDKIFQSNFFGGIAINDALPALGHTVWNVLAVYAEPEVSP
                     PITVVSGQPNQTIVYPNNVALGSKFPVQRMTAEQ"
     gene            complement(411..>1199)
                     /locus_tag="PL106_15065"
     CDS             complement(411..>1199)
                     /locus_tag="PL106_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34447.1"
                     /translation="APSTKPTDMSMVPMDQVAALVQKLVQEQLAQVPQAPAQGNAGIT
                     PETLKALMQEVRSEAIGEDGLVPEGYVPPEDYIETQKFFVPFARYYVYGKEVGPVHER
                     LPYNMKFLRFEPKFAYYTKENGVMHHKHIAILEVSSRKVYEYITGETLDGKKVGTPLP
                     EYGRVIFKDAGAAAASEQTAFAQMVQKHYTLLARKPLHELVKQYQGMGGATSSAWGIS
                     DYAWAIASQTAEREMENIHAGAKRAQAERDAARLLLKQEGVAVG"
BASE COUNT          208 a          366 c          370 g          255 t
ORIGIN      
        1 ctgctcggcc gtcatgcgct gcacggggaa cttgcttcca agggcaacgt tgttcgggta
       61 cacgatggtc tggttgggct gccccgacac cactgtgatg ggcggggata cctctggttc
      121 ggcgtacacc gccagtacgt tccacaccgt gtggcccagc gccggaagag cgtcgttgat
      181 ggcaatgccg ccgaagaagt tggactggaa gatcttgtcc tccttcaact cccacagcat
      241 ctcctcggag cccttgcgat tggccaaggc ccagcccccg gcggtaatgg cgcgagagat
      301 ggccgagttg atcgcgggct tcttgtcttg gtcaaagagg tagcgttgcg acccggttgc
      361 gtcaagcccc gcgtcgatca actcatcaag ctcttggacc gtgaacatga tcagccaaca
      421 gctacgcctt cttgtttgag cagcagacga gcagcgtcac gttcggcttg ggcgcgtttg
      481 gcaccagcgt ggatgttctc catctcgcgc tcggccgtct gggaagcgat ggcccatgcg
      541 tagtcggaga tgccccacgc cgaggatgtc gcgccaccca tgccctgata ctgcttcacc
      601 agctcgtgca ggggtttgcg ggccagcagc gtgtagtgct tctgcaccat ctgagcgaaa
      661 gctgtctgct cggaagcagc agcagcgcca gcatccttga agatcacgcg accatactcc
      721 gggagaggcg tgccgacctt cttgccgtcg agggtctcgc cggtgatgta ctcgtacacc
      781 ttgcggctgg acacctccag tatagcgatg tgcttgtggt gcatcacacc gttctccttg
      841 gtgtagtagg cgaacttcgg ctcgaagcgc aggaacttca tgttgtaggg caggcgctca
      901 tgcaccgggc ctacttcctt gccgtacacg tagtagcggg cgaagggcac gaagaacttc
      961 tgggtctcga tgtagtcctc cgggggcaca tagccctccg gcaccagccc atcctcacca
     1021 atggcttccg agcgcacctc ctgcatcagg gccttgaggg tctcgggggt gatcccggcg
     1081 ttaccctgtg cgggtgcttg gggtacctga gccaactgct cctgtacgag cttctggacc
     1141 aaggccgcca cctgatccat gggcaccatg ctcatgtcgg tcggcttggt tgaaggggc
//
LOCUS       DAOQOC010001141         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_110429_length_1195_cov_3.744737, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001141 DAOQOC010000000
VERSION     DAOQOC010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110429_length_1195_cov_3.744737"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..>1195
                     /locus_tag="PL106_15070"
     CDS             91..>1195
                     /locus_tag="PL106_15070"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNA34448.1"
                     /translation="MRKTFWTVSIVLSSLIAMAAWADQRWLWAYAVVGPLILLGMYNM
                     VQSRHTILRNFPVLGYARYFFEFISPEIQQYFIERHTDGRPFSRQQRSLAYQRAKNVM
                     DTVPFGTQLDINAANYEGIRHSIYPKAHPGEPPRIRIGGAFCTRPYEASLLNISAMSF
                     GALSDRAVLALNTGAKKGGFYHNTGEGGLSDHHLQAGGDLVWQIGTGYFGCRDKDGRF
                     DAELFRSKVAAHPQVRMIELKLSQGAKPGHGGVLPAVKNTPEIARIRHLEPHTTVISP
                     PGHSSFSDATGLLRFIGTLRELSGGLPVGFKLCIGRTEEFTGICEKMVETGMRPDFIT
                     VDGAEGGTGASPLEFTDSVGMPIEPALMFVRRTL"
BASE COUNT          224 a          360 c          377 g          234 t
ORIGIN      
        1 ggtccggcac cgggacatca acgggccatg cgagcggatc aaagtggtca ccacatgcgc
       61 ttcctggcgc ttgtggttat cttcatcccc atgcgtaaga ccttttggac cgtcagcatc
      121 gtgctcagtt cgttgatcgc catggcggca tgggccgatc aacgctggct gtgggcatac
      181 gcggtggtag gtccgctgat cctgttgggc atgtacaaca tggtgcagtc ccgccatacc
      241 atcctgcgga acttccctgt gctggggtat gcacggtatt ttttcgagtt catttcaccg
      301 gagatccagc agtacttcat cgagcggcac acggacgggc gccccttcag ccgccagcaa
      361 cgatcgttgg cctaccagcg tgccaagaac gtgatggaca ccgtcccctt cggtacgcag
      421 ctggacatca acgcggcgaa ctatgaaggc atccgccatt cgatctaccc caaggcgcat
      481 cccggagaac cgccccggat caggatcggc ggggccttct gcaccaggcc gtacgaggca
      541 tcgctcctga acatctcggc catgagcttc ggtgcgctga gtgatcgtgc cgtactggcc
      601 ttgaacacgg gtgcgaagaa gggcggcttc taccacaaca ccggtgaggg aggactgagt
      661 gatcaccatc tgcaggccgg cggtgacctg gtatggcaga tcggtacggg ctacttcggt
      721 tgccgcgaca aggacggtcg tttcgatgcg gaactgttcc gcagcaaggt tgcagcacat
      781 ccacaagtgc gcatgatcga gctgaagctg tcccaagggg ccaagcctgg tcatggcggg
      841 gtgttgcccg ctgtgaagaa cacgccggag atcgcccgca tccgccatct ggaaccgcac
      901 accacggtga tctcgccgcc gggccacagt tccttcagtg atgccacggg cctgttgcgt
      961 ttcatcggta cgttgcgcga gctgagcggt gggttgcccg tgggcttcaa gctgtgcatc
     1021 ggccgcacgg aagagttcac ggggatctgc gaaaagatgg tggaaacggg tatgcgcccg
     1081 gacttcatca ccgtcgacgg cgcggagggc ggcacgggtg cctccccact ggaattcacc
     1141 gacagtgtgg gcatgccgat cgaaccggcg ctgatgttcg ttcgccgcac cctgg
//
LOCUS       DAOQOC010001142         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_110511_length_1195_cov_2.394737, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001142 DAOQOC010000000
VERSION     DAOQOC010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110511_length_1195_cov_2.394737"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="PL106_15075"
     CDS             complement(<1..>1195)
                     /locus_tag="PL106_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34449.1"
                     /translation="MARLGPDYDVRPFTYGTEAKEGIDISQHDGRTDIDRLFRAVYDR
                     FAGPDLGAVTIDGDGIYNTGRDPRLSAERLGVPVYTIALGDTTVRPDLVLRDVEHNRI
                     TYLGNEFPVSARVEARHFGGRATRVSILRDGKELVGKDLAVSADPMVVDIPFLIKADA
                     PGTMRLTVRVAVRDGESNVANNERTIHIDVLDDRQRILLLALAPHPDVAALRQSLEGL
                     EGYRTEVAYAESFQGAPEAYDLIVLHQLPSSKATIQPVLHRIVEKRIPTLTIIGQNTD
                     LGQLNALETGLSITNGQRIMNDAQAAVNTDFALFTLDPEEVRAYERFPPLQVPIGTYT
                     AGRSAETLLFQKVGVVRTGYPLMVIDVRGERPAATICGEGLWRWRLADQQMYGGTERS
                     DQLVRK"
BASE COUNT          217 a          370 c          378 g          230 t
ORIGIN      
        1 cttgcggacc agctgatcgg accgttccgt tcctccgtac atctgctgat cggccagccg
       61 ccagcgccac agtccttcgc cacagatggt ggccgcaggc cgttcgccgc gcacatcgat
      121 caccatgagc gggtatccgg tgcgcacaac acccactttc tggaagagca gggtctcggc
      181 agaacgcccc gcagtgtacg tgccgatcgg gacctgcaag ggcgggaaac gttcatatgc
      241 gcgcacttcc tccggatcga gcgtgaacaa cgcgaaatcg gtattcacgg ccgcttgggc
      301 atcgttcatg atccgctgtc cattggtgat ggataggccg gtttccaagg cgttcaactg
      361 gcccaggtcg gtgttctgcc cgatgatggt cagcgtgggg atccgcttct ccacgatacg
      421 gtgcaacacg ggttggatgg tcgccttgct ggagggaagt tgatgaagca cgatgaggtc
      481 gtacgcttca ggggcccctt gaaagctctc ggcatacgcc acctctgtgc ggtaaccctc
      541 cagcccctcg aggctctggc gcaatgccgc cacgtcgggg tgcggtgcca gggccagcaa
      601 caggatgcgt tgccggtcgt ccagcacatc gatgtggatg gtgcgctcgt tgttggccac
      661 attggactcc ccatcgcgca cggccacgcg cacggtgagc cgcatggttc cgggtgcatc
      721 ggccttgatc aggaagggaa tatccacgac catgggatcg gcggatacgg ccaggtcctt
      781 gcccaccagt tctttcccat cgcgcaggat ggaaacgcgt gtggcacgac cgccgaaatg
      841 acgggcctcc acgcgggctg aaacagggaa ctcattcccg aggtaggtga tccggttgtg
      901 ttccacatcc cgcaagacca gatcgggccg taccgtggta tcgccgagtg cgatggtgta
      961 cacgggcacg ccgaggcgtt ccgcggacag gcgagggtcg cggcccgtgt tgtagatgcc
     1021 atcgccatcg atggtcaccg ccccgaggtc gggcccagcg aagcggtcgt acaccgcacg
     1081 gaagagccga tctatatcgg ttcggccatc gtgttgcgaa atatctatcc cttccttcgc
     1141 ttcggtaccg tacgtgaagg gccgcacatc atagtcgggg cccagcctgg ccatg
//
LOCUS       DAOQOC010001143         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_110545_length_1195_cov_2.107018, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001143 DAOQOC010000000
VERSION     DAOQOC010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110545_length_1195_cov_2.107018"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PL106_15080"
     CDS             <1..294
                     /locus_tag="PL106_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34450.1"
                     /translation="QWARDLEKSAERELDEAKAKIRELERKARQAGTMDEQRAAEQEI
                     AQWEKRKRDLRQRIFQVEDEIAQKRDRLIQALVQKMQQSSQTETLFTIRWRVV"
     gene            353..>1195
                     /locus_tag="PL106_15085"
     CDS             353..>1195
                     /locus_tag="PL106_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34451.1"
                     /translation="MKYELTIHDAAEPNGTIDMVRLARIAQGITKVADGALQLRLRGL
                     SFGPGRPSKDKLALENAMRITLTGMRSGSTVLDIECDTFEETLPNIQLDAFRAEGQTD
                     LKKTTPMGLFIETYHIALDNDGRKDLLDKNLLKELREFRKAFRGPDERMTIANRGSVK
                     KLELDKHAFERIHDLEVAIPEPSLTVVNGHLDMIRHSKALIEIKPGKGKPIEGFLGKD
                     LTMEDVQRFFGRAVTVKGLLHVRGDGKRLVEVQRLFEPTAGDAYFSRNAASETVVQQL
                     QRQVL"
BASE COUNT          289 a          373 c          351 g          182 t
ORIGIN      
        1 caatgggccc gcgacctgga gaagagcgcc gagcgcgaac tggacgaggc caaggccaag
       61 atccgcgagc tggagcgcaa ggcccgccag gccggtacca tggatgagca gcgcgccgcc
      121 gagcaggaga tcgcccagtg ggagaaacgc aaacgcgacc tccgccagcg catcttccag
      181 gtggaggacg agatcgccca aaaacgcgac cgcctcattc aagccctggt gcagaaaatg
      241 cagcagtcca gccagaccga aaccctcttc accattcggt ggagggtggt gtaggccaaa
      301 acgctgtccg taactttgaa cgaccttaaa gaccacgacc accgggccga acatgaaata
      361 cgaactcacc atacatgatg cggccgaacc caacggcacc atcgatatgg tgcggttggc
      421 gcgcattgcc cagggcatca ccaaagtggc cgatggcgct ttgcagctcc gtttgcgcgg
      481 actcagtttc gggcccggcc gtccatccaa ggacaaactc gccttggaaa atgccatgcg
      541 catcaccttg accggtatgc gttccggaag caccgtgctc gatatcgagt gcgacacttt
      601 cgaagagaca ctgcccaaca tccaactgga tgccttccgc gccgaagggc agaccgacct
      661 gaagaagacc acgcccatgg gcctcttcat cgagacctac cacatcgcat tggacaacga
      721 tgggcgcaag gacctgctgg acaagaacct cctgaaggaa ttgcgtgaat tccgaaaggc
      781 attccgtggg cccgatgagc gaatgaccat cgccaaccgg ggcagcgtga agaaactgga
      841 actggacaag cacgccttcg agcggatcca tgacctggag gtggccatcc ccgagccatc
      901 ccttaccgtg gtcaatggcc acttggacat gatccggcac agcaaggccc tgatcgagat
      961 aaaacccgga aaaggcaagc ccatcgaggg cttcctgggc aaggacctca ccatggagga
     1021 tgtgcagcgc ttcttcggcc gcgccgtcac cgtgaagggc ctgctgcatg tgcggggtga
     1081 tggcaaacgc ctggtggaag tgcagcgcct gttcgagccc accgccggtg atgcctactt
     1141 cagccgcaat gccgccagcg aaaccgtggt gcaacagctg caacggcaag tgctc
//
LOCUS       DAOQOC010001144         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_111429_length_1190_cov_3.487225, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001144 DAOQOC010000000
VERSION     DAOQOC010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111429_length_1190_cov_3.487225"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1190
                     /locus_tag="PL106_15090"
     CDS             <1..>1190
                     /locus_tag="PL106_15090"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNA34452.1"
                     /translation="NNLIDKYNQDVQQLRTNELNVKIATAPIIALRRTVQTERDQIVQ
                     SAQALVHQTQSELGALQSRISVLQGKLYVLPQQDAQRRIATKSYELTEGIHNYLMEKS
                     YEAQIAVNSDQVDKSVVDSARPDPPSPIAPDKKVVLGGAFAIGFIIPLLLLMLMDLVN
                     DKIGGLDELKRLTAIPILATIPSSKRKRITPDEPKSLLAESFRTARINLQYLNANAPR
                     QVVGMTSSTSGEGKTFCSINLATVITLSGKRTLLIDADMRRPRVHEYLELPDGPGLST
                     LLIGECTVEQAIRRSGIQGLDVITAGPIPPNPLELVESPRMAELFAKARERYDHIVVD
                     ASPMGLVSEFKIIMGHVDVILYVVRQGHTRRPMLRNVNELYHDGKLPHANMILNDVKA
                     GEGY"
BASE COUNT          274 a          381 c          346 g          189 t
ORIGIN      
        1 gaacaacctg atcgacaagt acaaccagga tgtgcagcag ctccggacga atgaactcaa
       61 tgtgaagatc gccaccgcac ccatcatcgc cctgcgccgc accgtgcaga ccgagcggga
      121 ccagatcgtg cagagcgcac aagccctggt gcatcagacc cagagcgaac tcggcgcctt
      181 gcagtcgcgc atcagcgtgc tccaaggcaa gctctacgtg ctcccccagc aggatgccca
      241 gcgccggatc gcgaccaaga gctacgaact caccgagggc atacacaact acctgatgga
      301 gaaatcctac gaggcgcaga tcgcggtgaa cagcgaccag gtggacaaaa gcgtggtgga
      361 cagtgcgcgc ccggacccac cctcccccat tgcgcctgac aagaaggtgg tactgggcgg
      421 tgccttcgcc atcggcttca tcatcccctt gctgctgctc atgctgatgg acctcgtgaa
      481 cgacaagatc ggtgggctgg acgaactgaa gcgattgacc gcgatcccta tcctggccac
      541 cattccgagc agcaagcgca agcgcatcac cccggacgaa ccgaaaagcc tgctggcgga
      601 gagtttccgc accgcccgca tcaacctgca atatctgaac gccaatgccc cgcggcaggt
      661 ggtgggcatg accagcagca ccagtgggga aggcaagacc ttctgctcga tcaacctggc
      721 cacggtgatc accctcagcg gcaaacgcac cttgctgatc gatgccgaca tgcgtcgtcc
      781 gcgtgtacac gagtacctcg agttgcccga tggtccgggc ctgagcacct tgctgatcgg
      841 ggaatgcacc gtggaacagg ccatccggcg cagcggcata caggggctgg atgtgatcac
      901 cgccgggccc atcccgccga acccgctgga actggtggaa tccccgcgca tggccgaact
      961 cttcgcaaaa gcccgtgaac ggtacgatca tatcgtggtg gacgcctcgc ccatgggact
     1021 ggtgagcgag ttcaagatca tcatgggtca tgtggatgtc atcctgtacg tggtacgcca
     1081 gggccacacg cgccgcccca tgttgcgcaa cgtgaacgaa ttgtaccacg acggcaaact
     1141 gccgcatgcg aatatgatac tcaacgacgt gaaggcaggt gaaggatacg
//
LOCUS       DAOQOC010001145         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_111860_length_1188_cov_2.558694, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001145 DAOQOC010000000
VERSION     DAOQOC010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111860_length_1188_cov_2.558694"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PL106_15095"
     CDS             <1..442
                     /locus_tag="PL106_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin protein"
                     /protein_id="HNA34453.1"
                     /translation="NGESPLGFCTTAGTYRVAVRHRNHLGVMTGQGIVLGNTPTGIDL
                     TSAATGTYGTNARKASGSVRLLWAGNSTGDVQVKYTGPSNDRDPILLAVGSTTPNASV
                     SGYLRTDVNMDGVAKYTGSGNDRDPILLNVGNTTPNATRQEQLP"
     gene            502..>1188
                     /locus_tag="PL106_15100"
     CDS             502..>1188
                     /locus_tag="PL106_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl carboxypeptidase II"
                     /protein_id="HNA34454.1"
                     /translation="MKHTSFAILPIALAVACGAPEQKAEPTVDTATANPLAAKSTLVF
                     QAPLFNLIKDSDYEPAIEAGMKKQLEEVRVIVENKEAPSFENTIVAMEKSGQDLTRAA
                     KIFFGLTGSATNDTLQAVKARLAPKLTAHGDAITMNDALFQRVKAVHDGLATDSMDPV
                     RTRLVERYFTRFKRAGALLDAKGKEELMKLNEEEANLNTRFQDNILKARSAAAVLVDK
                     KEDLDGLDEEA"
BASE COUNT          270 a          382 c          357 g          179 t
ORIGIN      
        1 caatggcgaa agtccgctgg gcttctgcac gacggccgga acctatcggg tggccgtgcg
       61 gcaccgcaac catttgggcg tgatgacggg ccagggtatc gttctgggca acacacccac
      121 gggtatcgac ctcacttccg cggccacggg gacatacggc accaacgcgc gcaaggcgag
      181 cggatccgta cgcctgctct gggcgggaaa cagtaccggt gatgtgcagg tgaaatacac
      241 cgggcccagc aacgatcgcg accccatcct gctggccgtg ggcagcacca cgcccaacgc
      301 cagtgtgagc ggctatctgc gcaccgatgt gaacatggat ggtgtggcca agtacaccgg
      361 cagcggcaac gaccgcgacc ccatcctctt gaatgtgggc aacaccacac ccaacgccac
      421 ccggcaggag cagcttcctt gaagcgacga acagttcagg agttcacgat cgttgagtga
      481 cggccgtgca tctttgcggc gatgaaacac acgtctttcg ccatccttcc gatcgccctc
      541 gccgtggcct gtggcgcacc ggagcaaaaa gccgaaccca ccgtggacac cgccaccgca
      601 aaccctcttg ccgccaagag caccctcgtg ttccaagcgc ccctgttcaa cctgatcaag
      661 gactccgact acgagcccgc cattgaagcg ggaatgaaga agcaactgga ggaggtccgc
      721 gtcattgtcg agaacaagga ggcaccgagc ttcgagaaca ccatcgtggc gatggagaag
      781 agcgggcagg atctgacccg tgcggcgaag atcttcttcg gcctcaccgg cagcgccacc
      841 aacgataccc tccaggccgt gaaggcccgc ctggccccga aactcacagc ccacggcgat
      901 gccatcacca tgaacgacgc gctcttccag cgggtgaaag ccgtgcatga tggccttgcc
      961 accgacagca tggatccagt gcggacccgc ttggtcgagc gctatttcac ccgcttcaaa
     1021 cgcgccggag ccctgctcga tgcgaaaggg aaggaggaac tgatgaagct gaacgaggag
     1081 gaggccaatt tgaacaccag attccaagat aatatcctca aggcgcggtc ggcggccgct
     1141 gtgctggtgg acaagaagga ggacctcgac ggcctggacg aggaagcc
//
LOCUS       DAOQOC010001146         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_112092_length_1187_cov_2.348940, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001146 DAOQOC010000000
VERSION     DAOQOC010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112092_length_1187_cov_2.348940"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PL106_15105"
     CDS             complement(<1..1146)
                     /locus_tag="PL106_15105"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNA34455.1"
                     /translation="MMTQGRTIDNQVRHTAGRLALLAGCAAVLGWMGVPDLAMAQDAA
                     AQAPTAYRDIPYVGSRNLVWIIAQLHLLLAGFVLGVPIFAWLCEVVGWKSGDKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIGFYPKLMAYLTDIFFPSFLVYCGLFLVETAT
                     LYLYWYGWDAMSEGNGKKFHIFLGFLLNVFAFFIMIVPNSWATFQASPVVIAEGTDFE
                     RAWAATWNPTWWPVNIHRIIANVVLGGYICGAYAGIRYLSAKSTEERYHYDWMGYVGN
                     FIGVFGLLPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWMGITHRIPGSEGQYRKPIMAMLIILLMCLAVWMTPHSLVASLEEAQ"
BASE COUNT          311 a          345 c          319 g          212 t
ORIGIN      
        1 ctgggcctct tccaagctgg cgaccagaga gtgcggagtc atccacacgg caagacacat
       61 cagcaggata atcaacatcg ccataattgg cttgcgatac tgcccctctg accctggtat
      121 tcgatgggta atacccatcc agaaatagta gttggatcca aggaagagaa cgccaatcaa
      181 catggcttgc aggatgaaca accaggaaag gaatccgccc atgagcgtga tgcccatctg
      241 ctggttgtat tggtagattt cgcgcatcaa ccaatagccg gcgaaaggca agggcaacaa
      301 accaaagacg ccgataaagt taccaacgta gcccatccaa tcgtagtgat agcgctcttc
      361 cgtgctcttg gcggacaggt agcgaatacc cgcgtacgct ccgcagatgt accctcccaa
      421 gacaacgttc gcgatgatgc ggtgaatgtt caccggccac caggttggat tccaggtggc
      481 agcccaggca cgctcgaaat cggtgccttc agcgatcacc accggactcg cctggaacgt
      541 ggcccacgag ttgggaacaa tcatgatgaa gaaagcgaac acgttgagaa ggaagcccaa
      601 gaagatatga aactttttgc cattcccctc ggacatcgcg tcccaaccgt accaatacag
      661 gtacagggtg gcagtctcga cgaggaagag tccgcaatag accaggaacg aggggaaaaa
      721 gatgtccgtc aaatacgcca tcagcttcgg atagaagccg atcaagagga acagcaggat
      781 gccaccgaac aaggccgtgg tggcatagga cgatgtgagc agcttggtga actctttcgc
      841 caacttatcg taccgcttgt caccggactt ccagccaacc acctcgcaca accacgcgaa
      901 gatcgggacg cccagcacga atccggcgag aagaaggtgc aactgcgcaa tgatccacac
      961 gagatttcgg cttccaacat aggggatgtc ccgataggcg gtcggcgctt gagcagccgc
     1021 atcctgggcc atggccaagt ccggaacgcc catccagccg agcactgcgg cgcatccggc
     1081 cagcaaggcg agacgccctg ccgtgtggcg cacctgatta tcgatagtcc gcccttgggt
     1141 catcatggtt gtcacctcgt cgtgcgccat catgtcactt actcccc
//
LOCUS       DAOQOC010001147         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_112388_length_1185_cov_4.980531, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001147 DAOQOC010000000
VERSION     DAOQOC010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112388_length_1185_cov_4.980531"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..549)
                     /locus_tag="PL106_15110"
     CDS             complement(202..549)
                     /locus_tag="PL106_15110"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNA34456.1"
                     /translation="MPFRIKRIYDRPTRTDGYRVLVDRLWPRGIRKDDARLNEWNKVL
                     PPSNALRKDYHDGRLDFRTFSAAYRKELQDHPEELDRMHELAEGRTVTLLFASSNAKE
                     NHAIVLLDVLQGK"
     gene            806..>1185
                     /locus_tag="PL106_15115"
     CDS             806..>1185
                     /locus_tag="PL106_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNA34457.1"
                     /translation="MKKTSAGPSHTTFTKEEAVARAMVYFKGDELAATTWVNKYALRN
                     ADGELLEDTPTSMHRRLAREFARIEAKYAPLTRAKRALLSTYGRDREVLDEDRIFKLF
                     DGFRDVVPQGSVMASLGDPYRLAS"
BASE COUNT          233 a          367 c          358 g          227 t
ORIGIN      
        1 cgaaccggtg cgctcggcga tctccaccag gctcatccgg cgtttgttcc gaccttccaa
       61 cgcgatgagc accgcagccc ggatggcgta ttggcaggcg cgggagaaca tcgtggcgaa
      121 aatagcggtt ggacgcccgt ggcccgatgt cgcgttgtca aacttggctc cgggccgttc
      181 aggaaccgcg gaacatggac ctcacttccc ctgcaggaca tccagtagca cgatcgcgtg
      241 gttctccttc gcattcgacg aagcaaatag gagcgtcacg gtgcgccctt cggcgagttc
      301 atgcatacgg tccagttcct cgggatggtc ctgcaattcc ttccggtacg cggcactgaa
      361 ggtgcggaag tccagtcgcc catcatggta gtccttgcgc aaggcattcg atggtggcag
      421 caccttgttc cactcattca gccgggcatc gtccttgcgg atcccacgtg gccaaaggcg
      481 atccaccagg acccggtaac catcggtgcg cgtcggtcgg tcgtagatgc gttttatgcg
      541 gaagggcatg gtgggacaaa ggtatcgtcc gagcacggcg tttgagcgca tccatgtcct
      601 gctgctctct gaaaggcccg tccaccaacg gggacagcgc gtttctcatg ttccgcgcca
      661 tgatccatca cgggttgtcc gatcgttcac aacgcggtgg agatcgtacc atgaagatga
      721 cggtcgtcag tgttcgcgga aggcagcttt gccccccacg atcaagcaga acatgtcaac
      781 aaccatcacc ctacagcccc ccgccttgaa gaagacttcc gccggaccct ctcacaccac
      841 cttcaccaag gaggaggcgg tagcgcgggc catggtatac ttcaagggtg atgaactggc
      901 ggccaccaca tgggtgaaca aatacgcgct gcgcaatgcc gacggggagt tgctggagga
      961 cacgccgacc agcatgcatc gccgcctggc acgtgagttc gcgcggatcg aggcgaagta
     1021 cgccccactg accagggcga aacgcgccct gctgagcacc tatggccgcg accgggaggt
     1081 gcttgacgag gatcgcatct tcaaactgtt cgatggcttc cgcgatgtgg tgccgcaggg
     1141 cagcgtgatg gcctcgctgg gcgatcccta ccgcctggca tcatt
//
LOCUS       DAOQOC010001148         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_112434_length_1185_cov_3.037168, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001148 DAOQOC010000000
VERSION     DAOQOC010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112434_length_1185_cov_3.037168"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..804)
                     /locus_tag="PL106_15120"
     CDS             complement(103..804)
                     /locus_tag="PL106_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA34458.1"
                     /translation="MRMDSLAAEAYILNRLRTGLPVHRSYHSFDHTLDVFRTASRIAA
                     AEGVAGEDLELLKAAALFHDSGFLVSDVEHERFSCGITREVLPGFGYSPKQVERICVM
                     IMATKIPQDPHNALSRILCDADLDYLGRPDFFSIGATLFEEMKHYGVLRTEREWNELQ
                     VRFLRKHHYFTQYSKSLREPVKLVHLDQVHVAERQPGARQRHRRSQDRRHEQLLAGVE
                     RGVGVRPDVGDGRVA"
     gene            867..>1185
                     /locus_tag="PL106_15125"
     CDS             867..>1185
                     /locus_tag="PL106_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34459.1"
                     /translation="MRTKDPGIGRHLNPMILIGVLGLYVMLQLGWWSWSLVQRDREVR
                     TLQEQLLAEGVAPGVPAHPPERVLWMVVGEGGVFAILMLFALWVIMKVLRHEMELARQ
                     QRDF"
BASE COUNT          218 a          377 c          362 g          228 t
ORIGIN      
        1 gtcttcgaca agcgctatgg cagcgtgacg gcggggaacg cctcccccct caccgatggc
       61 gccgccgccg tcctcctcat gagcgaggag cgcgccaagg cgttaggcta cccgcccatc
      121 gcctacatcc ggtcgtacgc ctacgccgcg ctcgaccccg gcgagcagct gctcatggcg
      181 ccggtcctgg ctgcgccggt ggcgctggcg cgcgccgggt tgacgctccg cgacatggac
      241 ctggtcgaga tgcacgagtt tcactggctc tcgcaaagac ttgctgtact gggtgaaata
      301 gtggtgcttg cgcaggaaac ggacctgcag ttcgttccat tcgcgctcgg tacgcagcac
      361 gccgtagtgc ttcatctcct cgaacaaggt ggctccgata ctgaagaaat ccggccgccc
      421 caggtagtcc aggtccgcat cacaaaggat acgcgagagc gcattgtgcg gatcctgcgg
      481 gatcttggtg gccatgatca tcacacagat ccgttccact tgcttcgggg aatagccgaa
      541 gccgggaagg acctctcggg tgatcccgca actgaaccgc tcatgctcaa catcggacac
      601 caggaaaccc gagtcatgga ataacgccgc ggctttcagc agttcaaggt cctcccccgc
      661 cacaccttcc gcagcagcga tccggctggc cgttctgaac acatcaaggg tatggtcgaa
      721 gctgtgatag ctccgatgga cgggcagacc cgtccgcaat ctgttcagga tatatgcttc
      781 ggccgcgagg gagtccattc gcatggggag cgcggcaata tagccgcctg tacgttcgga
      841 ctccccatct ttcaccccaa gtgggtatgc gcaccaagga ccctggcatt ggccgtcacc
      901 tgaaccccat gatcctgatc ggggtattgg gcctgtacgt catgctgcaa ctgggctggt
      961 ggtcctggtc actcgtgcaa cgcgatcgtg aagtgcgcac cctgcaggaa caactgctcg
     1021 ccgagggcgt cgcaccgggg gtacccgcgc atccgcccga gcgggttttg tggatggtgg
     1081 tcggcgaagg tggtgtgttc gcgatcctga tgctcttcgc cctctgggtc atcatgaaag
     1141 tcctgcggca cgagatggaa ctggcgcgcc agcaacgcga cttcc
//
LOCUS       DAOQOC010001149         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_112441_length_1185_cov_2.896460, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001149 DAOQOC010000000
VERSION     DAOQOC010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112441_length_1185_cov_2.896460"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PL106_15130"
     CDS             complement(<1..1035)
                     /locus_tag="PL106_15130"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNA34460.1"
                     /translation="MCGLLYSTASNAQLLGGEVHGNFSMDGQYYNADEVINAQKPPAT
                     WGLNSWGNINYTTSNGVFKAGMRFEAYEPSLLGYPAGQPYKGSGIGYRYATFNKYGLE
                     VTVGNFYEQFGQGLSFRSYEERYLGVDNAMDGVRLKFTPDTGIVLKGFIGQQRFGFEN
                     GASKLSAGQGIVRGFDAEVSLVEAWPRFFPKWGEKGHNLVLGGSFVSKFQSDKNSQLV
                     LPENVGVWAGRLNYTNAKWNVYAEYAHKINDPNLSNGFIYKDGQALLMNATYSTKGLG
                     ISAGAHSYDNMVFQSDRAAPNLFNLNINFLPPLAKQHTYNLPATLYPYATQPNGEVGF
                     AGEVFYKFKKG"
BASE COUNT          221 a          339 c          383 g          242 t
ORIGIN      
        1 gcccttcttg aacttgtaga acacctcgcc ggcgaagccc acctcgccgt tgggctgggt
       61 ggcgtagggg tacagggtgg cgggcaggtt gtaggtgtgc tgcttggcca gcgggggcag
      121 gaagttgatg ttgaggttga agaggttcgg agcggcgcgg tcgctctgga acaccatgtt
      181 gtcgtaggag tgcgcgccgg cggagatgcc aaggcccttg gtgctgtagg tggcgttcat
      241 cagcagcgcc tggccgtcct tgtagatgaa cccgttgctg aggttggggt cgttgatctt
      301 gtgcgcgtac tcggcgtaca cgttccactt ggcattggta taattcagtc gaccggccca
      361 tacgcccaca ttctcgggca ggaccaactg gctgttcttg tcgctctgga acttgctcac
      421 gaagctgccg ccgagcacga ggttgtgtcc cttctccccc cacttgggaa agaaacgggg
      481 ccaggcctcc accaaggaga cctcggcgtc gaagccgcgc acgatgccct ggccggccga
      541 gagtttggaa gccccgttct cgaagccgaa ccgttgctgc ccgatgaagc ctttgagcac
      601 gatccccgtg tccggcgtga atttcaaacg gacaccgtcc atggcattgt ccactccgag
      661 gtagcgctcc tcgtaactgc ggaaggaaag accctggccg aactgctcat agaaattgcc
      721 cacggtgacc tccagaccgt acttgttgaa ggtggcatag cggtagccga taccgctgcc
      781 cttgtagggc tgtccagcag gataacccag caaggaaggc tcgtacgcct cgaaccgcat
      841 gcccgccttg aacaccccgt tggaggtggt gtaattgatg ttgccccatg agttcagacc
      901 ccaggtggcc ggtggtttct gcgcgttgat cacctcgtcc gcgttataat actggccgtc
      961 catgctgaaa ttgccatgga cctcgccacc gaggagttgg gcgttggagg cggtactgta
     1021 cagaaggccg cacaagggca ggcatacccg ggcggcaaat ggatctcgaa gggtcatcgg
     1081 aggccgtggc gtggaagggg cgtgaaagta cggagggccg tggcatacga ccacggccct
     1141 ccgacaagga tgctggggat cctacttgcc cgccagttcc ttcac
//
LOCUS       DAOQOC010001150         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_113026_length_1182_cov_3.278616, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001150 DAOQOC010000000
VERSION     DAOQOC010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113026_length_1182_cov_3.278616"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1182)
                     /locus_tag="PL106_15135"
     CDS             complement(<1..>1182)
                     /locus_tag="PL106_15135"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNA34461.1"
                     /translation="YMGQGAWLLSSAGPELGERNPFYAIVPQWFLLSSVAIATLAAVV
                     ACQAVISGSFTLINEAITLNFWPRVRVKFPSEIRGQIYIPSVNWMLFVGCMGVMLYFR
                     QSSAMEAVYGFFIVVAMLMTSTLMFGYLRFAKRWPLFAVGAVLFVFFSMEIANFIANA
                     VKIIHQPLLLPSAMGLLFVMFIWYRARKITNRFLHFVPLTKYAQALRDLSDDKEIPKF
                     ATHLVYLTKADRATNVEKQVIDSILARKVKRADIYWFVHVNYTDEPYRMEYRVKELID
                     DKVIRVDFDLGFRVQARINMLFRRVLEEMESDHDLEFSSKYESIKKGDFHTDICYILL
                     DRVLSVENEFGLADDVVLDSYFALKKLALTDREAFGLDPNMTVVEKVPMIIQQRREVP
                     L"
BASE COUNT          246 a          358 c          347 g          231 t
ORIGIN      
        1 gcaacgggac ctcgcgccgt tgttggatga tcatcggcac cttttccacc acggtcatgt
       61 tggggtccag gccgaaggcc tccctgtcgg taagggccag cttcttcaat gcgaagtagg
      121 aatcgagcac cacatcatca gccaggccga attcattctc cacgctgagc acgcggtcca
      181 gcaggatgta acagatgtcc gtgtggaagt cgcccttctt gatgctctcg tacttgctgc
      241 tgaactccag gtcgtggtcg ctctccatct cctccagcac ccggcggaag agcatgttga
      301 tgcgcgcctg cacacggaat cccaggtcga agtcgacacg gatcaccttg tcgtcgatca
      361 gctccttcac gcggtattcc atccggtacg gttcgtcggt gtagttcacg tgtacgaacc
      421 agtagatgtc cgcacgcttc accttccggg caagaatgct gtcgatcacc tgtttctcca
      481 cgttggtcgc gcggtcggcc ttggtgaggt acaccaggtg cgtggcgaac ttggggatct
      541 ccttgtcgtc actgaggtca cgcagtgcct gggcgtactt ggtaagcggt acaaaatgaa
      601 ggaagcggtt ggtgatcttc cgggcgcggt accagatgaa catcacgaac agcaggccca
      661 tcgctgacgg cagcagcagc ggctggtgga tgatcttcac cgcattggcg atgaagttcg
      721 cgatctccat gctgaagaac acgaacagta ccgcacccac ggcgaagagc ggccagcgtt
      781 tcgcgaagcg caggtacccg aacatcaggg tactggtcat cagcatggcc accacgatga
      841 agaagccgta cacggcttcc atcgcgctgc tctgccggaa gtacagcatc acgcccatgc
      901 agcccacgaa aagcatccaa ttgacgctcg ggatatagat ctgcccgcgg atctcgctgg
      961 ggaacttcac tctcacccgg ggccagaagt tgagtgtgat cgcctcgttg atcagggtga
     1021 acgaaccact gatcaccgcc tggcaggcga ccaccgcggc cagggtggcg atcgccacgc
     1081 tcgacagcag gaaccattgg ggcacgatgg catagaaggg gttgcgctcg cccagctccg
     1141 gaccggcgga ggacagcagc cacgcaccct gccccatgta gt
//
LOCUS       DAOQOC010001151         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_113229_length_1181_cov_2.947602, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001151 DAOQOC010000000
VERSION     DAOQOC010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113229_length_1181_cov_2.947602"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PL106_15140"
     CDS             <1..998
                     /locus_tag="PL106_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34462.1"
                     /translation="INDANERRYMLEALSCVHEVHVGSGSGQMDFVPEFLHVKPDFLV
                     VNADGHAEAKAALCEQHGVRYVVLERTPHEGLKARSTTDLRATCTIPFRIDLAGGWLD
                     QPYVAKHHGGSVLTISIEPTHAFNDRSGMSSSTRKKAIELWGTHLPDGDREKHARLLF
                     ASENPPGTVEVSGSQDSIGIVYPGLNRLHYDGNYWPERIESVQDEDVLHFIEEHLQLV
                     PLEPRAPGFSVLEGTHIEAAGAKALADAADACWSAVLAKDAVAFGAAFRRSFEAQVAM
                     FPHMVDEDIRAQIARHADDALGWKLSGAGGGGYLILVRKETLPGTLRITIRRSGW"
     gene            1001..>1181
                     /locus_tag="PL106_15145"
     CDS             1001..>1181
                     /locus_tag="PL106_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HNA34463.1"
                     /translation="MGHTSQRTAIVLVTSLFFLWALAHNLNPILIPHLRKALQLNDAR
                     SALVDSAFFIAYFVMA"
BASE COUNT          225 a          393 c          380 g          183 t
ORIGIN      
        1 cgatcaacga cgcgaacgag cgccggtaca tgctggaggc actgtcctgc gtgcatgaag
       61 tgcatgtggg ctcgggcagc ggacagatgg atttcgtacc ggaattcctg cacgtgaagc
      121 ccgacttcct ggtggtgaac gccgacggcc atgccgaggc caaggccgcc ctgtgcgaac
      181 agcacggcgt gcgctacgtg gtgctggaac gcacgccgca cgaaggcttg aaggcacgca
      241 gcaccacgga cctgcgcgcc acatgcacca tcccgttccg catcgacctg gccggcggtt
      301 ggctggacca gccctatgtg gccaagcacc acggcggcag tgtgctcacc atcagcatcg
      361 aacccacgca cgccttcaac gaccgcagcg gcatgagcag cagcacccgc aagaaggcca
      421 tcgagctctg gggaacgcac ctgcccgacg gcgaccgcga gaagcacgcc cgcctgctct
      481 tcgccagcga gaacccgccc ggcacggtgg aagtcagcgg ctcgcaggac agcatcggca
      541 tcgtgtaccc ggggctgaac cgtctgcact acgacggcaa ctactggccg gagcgcattg
      601 aaagcgtaca ggatgaggac gtgctgcact tcattgagga gcacctgcaa ctggtgccac
      661 tggaaccgcg tgcaccgggc ttcagcgtgc tggagggcac gcacatcgaa gccgccggtg
      721 ccaaagcctt ggccgatgcc gccgacgcct gttggagcgc cgtgctggca aaggatgcgg
      781 tggccttcgg cgcggcgttc cgccgctcgt tcgaggcgca ggtggccatg ttcccccaca
      841 tggtggacga ggacatacgc gcgcagatcg cacggcacgc ggacgatgca ctgggatgga
      901 agctgagcgg cgcgggcggc ggtggttatt tgatcctggt gcgcaaggag accctgcccg
      961 gaacgttgcg catcaccatc agaagaagcg gctggtgacc atgggccaca cctcgcagcg
     1021 cacggccatc gtgctcgtca cgagcctctt cttcctgtgg gcactggcgc acaacctcaa
     1081 ccccatcctc atcccgcatc tgcgcaaggc gctccagttg aacgatgcgc gcagtgcctt
     1141 ggtggacagc gccttcttca tcgcctactt cgtgatggcc c
//
LOCUS       DAOQOC010001152         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_113315_length_1181_cov_2.091474, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001152 DAOQOC010000000
VERSION     DAOQOC010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113315_length_1181_cov_2.091474"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1181
                     /locus_tag="PL106_15150"
     CDS             <1..>1181
                     /locus_tag="PL106_15150"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34464.1"
                     /translation="DVQPGAGTVLVNNDAQYLLRYDSSFTYVAHTSEGFGEQRLAVDA
                     DGNLYMMARSNVGGDLWALKYSVSGQSIGQVYQTALTSLGNLRMADIVADGQGGALGS
                     YSNNCSPTSFYRFFKMNVSGLVDFNLSLYSGIDCTNPAAKGFDVRGGTFVVGSFNNSY
                     TVDFDPGTGAMVLPTGNDKGAVALYNWCSSSPFDPFGIDVVSTAWCVGDTLTLAADAF
                     GDASGYTWDAGAWSIAAQSGDMVQVIADNAGPVTVSLAAVNACGSSAPVTTQLTASNA
                     TADLPLDAIECFSFTGVLDPGPCAGCTYVWQPGGETTATLPVDITETTTFSVTATQGN
                     CSVSDSTTITIENCTGIAAAGTDGVVVGPVPVVRGQEVVVSGIAPGSLLRLSTLDGRY
                     V"
BASE COUNT          214 a          366 c          374 g          227 t
ORIGIN      
        1 cgatgtgcag cccggcgccg gcacggtact cgtgaacaac gatgcacaat acctgctgcg
       61 ctacgacagt tccttcacct acgtggcgca cacctcggaa ggcttcggtg agcagcgcct
      121 ggcggtggac gcggacggta acctctacat gatggcgcgg tcgaatgttg gtggcgactt
      181 gtgggcgttg aagtacagcg tctccggaca gtccatcggt caggtgtacc agaccgctct
      241 gaccagcttg gggaacttgc gcatggcgga tatcgtggcc gatggccaag gcggcgcatt
      301 gggttcatac agcaacaact gttcgcccac cagcttctac cgcttcttca agatgaacgt
      361 gagcggtctg gttgacttca atctatccct gtacagcggc atcgattgca ccaaccccgc
      421 tgccaaaggc ttcgatgtgc gcggcggcac cttcgtagtg ggttccttca acaacagtta
      481 cacggttgat ttcgatccgg gtaccggcgc catggtcttg cccacgggca acgacaaggg
      541 cgcagtggcc ctttacaact ggtgcagctc ctcgccgttc gaccccttcg gtatcgatgt
      601 ggtgagcacg gcgtggtgcg tgggcgatac actgaccttg gccgccgatg ccttcggcga
      661 tgcgagcggc tacacctggg atgccggtgc atggtccatc gccgcgcagt cgggcgacat
      721 ggtgcaggtg atcgccgaca acgccggtcc ggtgaccgtt tcactggcgg cggtcaacgc
      781 atgtggcagc agcgcgccag tgaccacaca actcaccgca agcaacgcca ccgccgatct
      841 gccgctcgat gcgatcgagt gcttcagctt caccggcgta ctggatcccg gcccttgcgc
      901 gggctgcacc tacgtgtggc aaccaggcgg ggagaccacc gccacgctcc ctgtggacat
      961 cacggagacc accactttct cggtaacggc cacgcaaggc aattgcagcg tgagcgacag
     1021 caccaccatc accatcgaga actgcacggg cattgctgct gcgggcacgg acggtgtggt
     1081 ggtaggtccc gtgcctgtgg tgcgtgggca ggaggtggtg gtgagcggga tcgcgccagg
     1141 aagcctactt cgtctttcca ccctggatgg ccgctacgtg g
//
LOCUS       DAOQOC010001153         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_113678_length_1179_cov_2.483986, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001153 DAOQOC010000000
VERSION     DAOQOC010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113678_length_1179_cov_2.483986"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /gene="dnaN"
                     /locus_tag="PL106_15155"
     CDS             complement(<1..895)
                     /gene="dnaN"
                     /locus_tag="PL106_15155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNA34465.1"
                     /translation="MKFIVSSSALLKQLQSLQGVLASSNTLPILDNFLFEIDGKQLTV
                     TASDLETTITATMPVEAKDKGVVAIPARLLLDTLKAFPEQPLTFSVDGKHYGIEISSD
                     QGTYKMTGHNGAEFPKSPVIEGGSSFSLPAGTLATAINKTIFATGNDDLRPVMSGIFF
                     ELAEDGIRFVATDAHKLVRYTRTDIKVPGTASFIVPKKPLNLLKNSLASTNAEVTVAY
                     NENNASFAFDNTVLVCRLIDGKYPNYEAVIPKTNPNKLTIDRQTFLNSVRRVAIFSNK
                     TTHQVRMNIAGSQLAVSAEDLD"
BASE COUNT          199 a          325 c          391 g          264 t
ORIGIN      
        1 aatcgaggtc ctcggcgctc accgcgagtt ggcttcccgc gatgttcata cgcacctggt
       61 gcgtggtctt gttgctgaag atggccacgc ggcgcacgct gttcaagaag gtctgccggt
      121 ctattgtgag cttgttcgga ttcgtcttcg ggatcaccgc ctcgtaattg gggtatttcc
      181 cgtcgatcag gcggcacacc agcacggtat tgtcgaaggc gaaggaggcg ttgttctcgt
      241 tgtaggccac ggtcacctcg gcgttggtgc tggccaagga gtttttcagc aggttcagtg
      301 gcttcttggg cacgatgaag ctggcggtac cgggcacttt gatgtcggtg cgggtgtaac
      361 gcaccagctt gtgtgcatcg gtggccacga agcgtatgcc gtcctcggcc agttcgaaga
      421 agatgccgct catcaccgga cgcaggtcgt cgttgccggt ggcgaaaatg gtcttgttga
      481 tggccgtggc gagtgttcct gcaggcaggg agaagctgct gccaccctcg atcaccgggc
      541 tcttggggaa ttcggcgccg ttgtggccgg tcatcttgta ggttccctga tcgctgctga
      601 tctcgatgcc ataatgtttg ccgtccacgg agaaggtgag gggctgctcg gggaacgcct
      661 tcagcgtgtc cagcagtaga cgggcgggaa tggccacgac gcccttgtcc ttcgcctcca
      721 cgggcatggt ggcggtgatg gtcgtctcca ggtcgctggc ggtcaccgtg agctgcttgc
      781 cgtcgatctc gaacaggaaa ttatcgagga tgggcagtgt attgctgctg gcgagcaccc
      841 cttggagcga ctgcaactgc ttcaacaggg ctgagctgga aacgatgaat ttcattcgga
      901 aaggttgggt tgatgcccac cgcgcgcagc acggtgttcg ttcaggcggc gcgaatttac
      961 cgggggggct tcccttggaa cggccctttt ataaacaagg acgcgggttt ttcaacgatc
     1021 cgcggtcgat ggcccgcgcg cggcggacgt catggccttt ggcgcacctg ccccaagtaa
     1081 ggcaccatgc ggtcgaacca gtagcgctgc accaccacca gggccgtgtc gtccttcacc
     1141 gagagcatgc ggtccacgtc cacggtcaag ggctcgaag
//
LOCUS       DAOQOC010001154         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_114247_length_1176_cov_3.845674, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001154 DAOQOC010000000
VERSION     DAOQOC010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114247_length_1176_cov_3.845674"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..>1176
                     /locus_tag="PL106_15160"
     CDS             125..>1176
                     /locus_tag="PL106_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA34466.1"
                     /translation="MRTTFFLLLPAILFSACGTGQPTGRSITAHVAGGAGRTLYLEKF
                     SANQPTRIDSVKLDGDGKGTLRPPVMPLDFYAVSMGENDRLILILDSTESVAFEAAAD
                     HLSDPESVTGSAHSMLMHDLFKEMKTYSTERQELVGRINADRNDSAAIASIERLNTDF
                     YTRCRDFVTKHTGSPAVLAAVQNLNIQNDLALFQQVRDSLGKTIPRSEYYVAFRDQVS
                     RMEQQLAAMKAQEEQMARLDNLIPVGSEAPDFAQPAPDGKNLSLSSLRGKVVLIDFWA
                     SWCKPCRMENPNVKRVYERFKGKGFEILGVSLDKDKNAWTGAIAQDGLPWKHVSDLAF
                     WNNAAAQQYGVSSIPYT"
BASE COUNT          269 a          376 c          361 g          170 t
ORIGIN      
        1 acaaagggct gctcggcctg ttcatgggcg aagtgatgaa agcgacccag ggcaaagccg
       61 acccgcgcaa ggcgaacgaa ctcgcacaaa agatcctcca ggagggcaac acatgatgag
      121 cacgatgaga acgaccttct tccttctact ccccgcgatc ctgttctccg cctgcggtac
      181 gggacagccg accgggcgca gcatcaccgc gcacgtggcc ggcggggccg ggcgaacgct
      241 ctatctggag aagttcagcg ccaaccagcc cacacgcatc gattcggtga aactcgacgg
      301 agatgggaag ggcacgctgc ggccacccgt gatgccgctc gacttctacg ccgtgagcat
      361 gggcgagaac gaccggctga tcctgatcct ggattccacg gagagtgtgg ccttcgaggc
      421 cgcggccgac cacttgagcg atcccgaaag cgtgacgggc agcgcgcaca gcatgctcat
      481 gcacgacctc ttcaaggaga tgaagaccta cagcaccgag cggcaggagc tggtgggccg
      541 catcaacgcg gaccgcaacg acagtgcggc catcgccagc atcgagcggc tgaacaccga
      601 tttctacacg cgctgccgcg atttcgtcac gaagcacacc ggtagcccgg ccgtcctggc
      661 cgccgtgcag aacctgaaca tccagaacga cctggcgctg ttccagcagg tgcgcgattc
      721 actgggaaaa acaattccca ggagcgagta ctatgttgcc ttccgcgacc aggtaagccg
      781 catggagcag cagctggcgg ccatgaaggc ccaggaggaa cagatggcac ggttggacaa
      841 cctgatcccc gtgggaagcg aagccccgga cttcgcccag cccgcacccg acggcaagaa
      901 cctctccctt tccagcctgc gcggcaaggt ggtgctcatc gacttctggg ccagctggtg
      961 caaaccctgc cgcatggaga accccaatgt gaaacgggtg tacgagcggt tcaaaggcaa
     1021 aggcttcgag atcctcggcg tgagcctgga caaggacaag aacgcatgga caggggcgat
     1081 cgcccaggac ggcctgccgt ggaagcacgt gagcgacctc gccttctgga acaacgcggc
     1141 cgcccaacaa tatggcgtaa gcagtatccc gtacac
//
LOCUS       DAOQOC010001155         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_114696_length_1174_cov_2.953530, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001155 DAOQOC010000000
VERSION     DAOQOC010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114696_length_1174_cov_2.953530"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PL106_15165"
     CDS             complement(<1..315)
                     /locus_tag="PL106_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA34467.1"
                     /translation="MHDHTNRLIHESSPYLRQHAHNPVDWYPWGEEAFAKAKAENKLV
                     LVSIGYSSCHWCHVMERESFENDSIAQLMNERYVCIKVDREERPDVDQVYMTAVQLMT
                     GRG"
     gene            497..1003
                     /locus_tag="PL106_15170"
     CDS             497..1003
                     /locus_tag="PL106_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34468.1"
                     /translation="MDEDDRQWPSWLMVTGIFGMLVAFWWTSEAVLVTYWTVGRLFCA
                     FAFSGNLLYGTTVRRLLGMERGYWFMFNLLFIGPVLFCLFFGLNGLFTHDTRTYIQRF
                     RIHPGPKAYWIEHGELPRAMLVEPGDTAVITHPIGMEDEHIRVLRLSHGLFGFEVMGW
                     GKPVYVVR"
BASE COUNT          180 a          378 c          367 g          249 t
ORIGIN      
        1 accacgcccg gtcatcagtt gcaccgctgt catgtacacc tggtccacat cgggccgctc
       61 ctcccggtcc accttgatgc acacgtaccg ctcgttcatc agttgcgcga tgctgtcgtt
      121 ctcgaagctc tcgcgctcca tcacgtggca ccagtggcag ctgctgtatc cgatgctcac
      181 cagcaccagc ttgttctccg ctttcgcctt ggcgaaggcc tcctcgcccc atgggtacca
      241 gtccaccggg ttgtgcgcat gctgccgcag gtaggggctg ctctcgtgga tgaggcggtt
      301 ggtatgatcg tgcatggtgc tgtccgcgcc ctggccgctg ttcccgttct tcgtggtgtc
      361 gcatccgggc acaagcaatg tgccaacgag gatcgcggac ccgcacagtt tggccaccaa
      421 cgccatgggc ccccaaagga acgttgaacg cgtacggccg cgtacttggc gacaactcgc
      481 gggccaacaa ccccacgtgg acgaggatga ccgccagtgg ccctcctggc tgatggtgac
      541 ggggatcttc ggcatgctgg tggccttctg gtggacgagc gaggccgtgc tggtgacgta
      601 ctggacggtg ggccgcttgt tctgtgcctt cgccttcagc ggcaacttgc tgtatggcac
      661 aacggtgcgg cgcctcctcg gcatggaacg cggctactgg ttcatgttca acctgctctt
      721 catcggaccg gtcctgttct gcctcttctt cgggctgaac ggcctgttca cgcacgatac
      781 gcggacatac atccagcgct tccgcatcca tccgggcccc aaagcctact ggatcgagca
      841 cggcgagttg ccccgcgcaa tgctggtcga gccgggtgat acggcagtga tcacgcatcc
      901 catcgggatg gaggatgaac atatccgcgt gctgcgcctc tcgcacgggc tgttcggctt
      961 cgaggtgatg gggtggggga agccggtgta cgtggtgcgt tgatggggct acctgcccag
     1021 gtccagttcg tgctccgcct cgaatgggat ctttttgcgg aggaggagtg cccgtgccgt
     1081 tacggtgcca ctggccttca cccggacctt gcctccgaag gccgcactga gcaaggcccc
     1141 catcgcgggc aggccacctc cgttgaagga tgcg
//
LOCUS       DAOQOC010001156         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_114777_length_1174_cov_2.187668, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001156 DAOQOC010000000
VERSION     DAOQOC010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114777_length_1174_cov_2.187668"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1174
                     /locus_tag="PL106_15175"
     CDS             <1..>1174
                     /locus_tag="PL106_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNA34469.1"
                     /translation="GMMIDPHVLREHWLPVLVLCVVTLIGQPLSSMSGAVLAGQPLKT
                     AVRAGMSLSQIGEFSFIIATLGLTLNVTSDLLYPVAVAVSAITTFTTPYMIKAGEPMH
                     ALIARLLPGRWLAGIAYYSDQSGQVRSISDWNKLLRAYLLNIALHTVIAVAIIALCAR
                     VVAPWTDGQGSFPGSGQLTGLITLVALGPSLWAIAVRRIQPVAYRNLWLNRRQLRGPL
                     VALELMRVLTGLLLIGLVVGIFFPSSWAMLAVPLVVIIALVIFRQRLQRFYQRVEKHF
                     LLNLNQRTDRARRQVPALAPWDMHPATMRVAEGSAAGGRTLAELALRERYGVNIARID
                     RDDRTINLPGANDRILPGDELLVIGTDEQLVRMDHALAQAPIAPASGHLTDGHIELL"
BASE COUNT          193 a          372 c          380 g          229 t
ORIGIN      
        1 gggcatgatg atcgacccgc acgtgctgcg cgaacactgg ctgccggtgc tggtactgtg
       61 cgtggtcacc ctgatcgggc agccgctcag cagcatgagc ggggcggtac tggccggcca
      121 acccttgaag acggcggtac gcgccggcat gagcctttcg cagatcgggg aattctcctt
      181 catcatcgcc acgctggggc tcaccctcaa cgtgacgagc gacctgttgt accccgtggc
      241 ggtggcggta tcggccatca ccacgttcac cacaccttac atgatcaagg cgggtgagcc
      301 gatgcatgca ttgatcgcac ggctgctgcc ggggagatgg ctggcgggga tcgcgtatta
      361 ttcggaccag agcggacagg tgcgttccat cagcgactgg aacaaactgt tgcgcgccta
      421 tctgctgaac atagcgttgc acacggtgat cgccgtggcc atcatcgcgc tctgcgcgcg
      481 ggtggtggcg ccttggaccg atgggcaggg atctttcccc ggcagcggcc aacttaccgg
      541 cctcattacc ctggtggccc tggggccgtc attgtgggcc atcgctgtgc ggcgcatcca
      601 gcccgttgcc taccggaacc tctggctcaa ccggcgccag ttgcgcgggc cgctggtcgc
      661 gctggaactg atgcgggtgc tcacaggatt gctgctcata ggcttggtgg tcggtatctt
      721 tttcccgtcc agctgggcga tgctggccgt gcccctggtg gtgatcatcg cactggtgat
      781 cttccgccag cggttgcagc ggttctatca acgcgtggag aagcacttcc tgctgaactt
      841 gaaccagcga acggaccgtg cgcgtcggca ggtgcccgcc ttggccccat gggacatgca
      901 cccggccacc atgcgggtgg ccgaaggctc ggctgcgggt ggccgtaccc tggctgaatt
      961 ggcattacgt gagcgttacg gggtgaacat cgcccgcatc gatcgggacg accgaacgat
     1021 caacctgccc ggtgccaacg atcggatcct tcccggggat gaactgctgg tgatcggcac
     1081 cgatgagcag cttgtacgca tggaccatgc ccttgcacag gcaccgatcg cacccgcatc
     1141 cggccacctc accgatggcc acatcgagct gctc
//
LOCUS       DAOQOC010001157         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_114929_length_1173_cov_2.838998, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001157 DAOQOC010000000
VERSION     DAOQOC010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114929_length_1173_cov_2.838998"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1173)
                     /locus_tag="PL106_15180"
     CDS             complement(<1..>1173)
                     /locus_tag="PL106_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNA34470.1"
                     /translation="RDIIVRANDEGEKIRLGDIADVTLGSEFFDIYSTMDGMPAASIV
                     LKQTMGSNASEVIAKVKAKLKDLSVMMPPSIKYRVSYDVSTFLDASTEQVLHTLRDAF
                     ILVALVVFIFLGDWRSTLIPIIAVPVSMIGTFFVMQMFSLSINLITLFALVLSIGIVV
                     DNAIVVVEAVHAKMEQEHLSPYNAVKVVMGEIGGAIIAITLMMVSVFIPIAFMTGPVG
                     VFYRQFSITMAGGIIISAVVALTLTPVLCAMMLKPHVAGHKKKNLLDRFIAAFNRGFE
                     RMTGRYVAILDRVVARRVLTFGSLAVFCAGIYISGQVLPAGFIPNEDQGTIYAIIQTP
                     PGSTLETTNKVAHNLQHICEEIPEIESVSSLAGYEIMTEGRGSNAGTCLINLKPWHD"
BASE COUNT          237 a          356 c          357 g          223 t
ORIGIN      
        1 gtcgtgccag ggcttcaggt tgatcaggca ggtacccgca ttggagcccc gaccctcggt
       61 catgatctcg tagccggcca gggaagaaac cgattcgatc tcggggatct cctcgcagat
      121 gtgctgcagg ttgtgggcca ccttgttggt cgtttccaac gttgatcccg gcggggtctg
      181 gatgatggcg tagatcgttc cctggtcctc gttcgggatg aaacccgcgg gcagcacctg
      241 gccggagatg tagatgcccg cgcagaacac ggcgagcgag ccgaaggtga gcacccggcg
      301 cgccaccacc cggtccagga tcgccacata gcgaccggtc atgcgttcga agccccggtt
      361 gaatgcggcg atgaagcggt ccagaaggtt cttcttctta tggcccgcca cgtgcggctt
      421 cagcatcatg gcacagagca cgggcgtgag cgtgagcgcg accacggcgc tgatgatgat
      481 gccgcccgcc atggtgatgg agaactgccg gtagaacacc cccaccggcc ccgtcatgaa
      541 ggcgatgggg atgaacacgg ataccatcat cagggtaatg gcgatgatcg caccgccgat
      601 ctcgcccatc accaccttca ccgcgttgta cggcgacagg tgctcctgct ccatcttggc
      661 atgcaccgcc tccaccacca cgatcgcgtt gtccaccacg atgccgatgg acagcaccag
      721 cgcgaaaagc gtgatcagat tgatcgagag gctgaacatc tgcatcacga agaaggtgcc
      781 gatcatggac accggaacgg cgatgatcgg gatcagcgtg gaccgccagt cgcccaggaa
      841 gatgaacaca acgagcgcca ccaggatgaa cgcatcgcga agcgtatgca gcacctgttc
      901 ggtggaagcg tccaggaagg tggatacatc gtagctcacc cggtatttga tgctgggtgg
      961 catcatcacc gagaggtcct tgagtttcgc cttcaccttg gcgatcactt cgctggcatt
     1021 gctgcccatg gtctgtttca gcacgatgga ggccgcgggc atgccgtcca tggtggagta
     1081 gatgtcgaag aactcgctgc cgagcgtcac gtccgctata tcgcccaacc ggatcttttc
     1141 cccctcatcg ttcgcgcgta cgatgatgtc cct
//
LOCUS       DAOQOC010001158         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_115313_length_1171_cov_3.139785, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001158 DAOQOC010000000
VERSION     DAOQOC010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115313_length_1171_cov_3.139785"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PL106_15185"
     CDS             <1..371
                     /locus_tag="PL106_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNA34471.1"
                     /translation="NFSAGSHTFTLTEAGTIWYVCTPHADMGMKGRIIVSTNTGVNDQ
                     LATSISLAPNPAMDVVRFTGVPGTARADLYDTKGALVLSHPVASGMLEVSSVLPGSYV
                     CVVRSTDGTLLLRTPLEIRR"
     gene            complement(383..646)
                     /locus_tag="PL106_15190"
     CDS             complement(383..646)
                     /locus_tag="PL106_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34472.1"
                     /translation="MRSLLFAAFTLLFLAWSNDLVAQGCAMCKATVQSGQEQSGVFGG
                     EQAVGEGLNAGILFLMTVPYVLLFLFLRKRIVGFLKEFASAKG"
     gene            complement(721..>1171)
                     /locus_tag="PL106_15195"
     CDS             complement(721..>1171)
                     /locus_tag="PL106_15195"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNA34473.1"
                     /translation="LNRVQVPAPAGLDPHVFARINAVINSAVSLLLIAGLFTAKAGNW
                     RMHRRVMLTAMVLSICFLISYLLHHLFAGETRFGGAGTIKIVYYVILATHILLAGLSL
                     PFILLTAYRALRADHPRHRQLARRVWPVWFYVSVSGVVVYLLISPYY"
BASE COUNT          244 a          365 c          365 g          197 t
ORIGIN      
        1 acaacttcag cgcgggcagc cacaccttca cactcaccga ggccggcacc atctggtacg
       61 tctgcacacc ccatgccgat atgggcatga agggacgcat catcgtgagc accaataccg
      121 gggttaacga tcagttggca acctccatct cgctggcacc gaaccctgcc atggatgtgg
      181 tacggttcac gggtgtgcca ggcaccgcgc gggccgacct gtacgacacg aagggtgcgc
      241 tggtgctttc ccacccggtg gcgagcggca tgctggaggt ttcctccgtg cttccgggga
      301 gctatgtatg cgtcgtacgc tccactgacg gcaccttgct gctgcgtacc ccgcttgaaa
      361 tacggcgctg aatcaaggct cctcagccct tggccgaggc gaactccttg aggaagccca
      421 cgatgcgctt gcgcaggaag aggaagagca gcacgtaggg aacggtcatc aggaagagga
      481 tcccggcatt gagcccttcg cccaccgctt gttcaccgcc gaacacgcca ctctgctcct
      541 gtccgctctg cacggtggcc ttgcacatgg cacagccttg tgctacaagg tcgtttgacc
      601 atgcgagaaa gagcagcgta aaagcagcga acagcaggct gcgcatcctg tacgatgtgc
      661 gcactaccgc ccgtggcctg ctatcccgct ttcgatgcct caacacggca cgaaagtacg
      721 tcagtagtat gggctgatca gcaggtacac caccaccccc gaaacgctca cgtagaacca
      781 cacgggccac acgcgcctgg ccaactggcg gtgccggggg tgatcggcgc ggagcgcgcg
      841 gtaggccgtg aggaggatga agggcagcga gagaccggcc agcaggatgt gcgtggccag
      901 gatcacgtag tagacgatct tgatggtgcc cgcgcctccg aaacgggtct ctcccgcgaa
      961 gaggtgatgc aggagatagg agatcaggaa acagatggag agcaccatgg cggtgagcat
     1021 cacccggcgg tgcatgcgcc aattgccggc cttcgcggtg aacaaccctg cgatcagcag
     1081 caggctcacc gcgctattga tcacggcgtt gatccgtgcg aatacatgcg gatccaggcc
     1141 tgcgggcgcg ggcacctgca cgcggttcag c
//
LOCUS       DAOQOC010001159         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_115515_length_1170_cov_3.098655, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001159 DAOQOC010000000
VERSION     DAOQOC010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115515_length_1170_cov_3.098655"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1126
                     /locus_tag="PL106_15200"
     CDS             92..1126
                     /locus_tag="PL106_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA34474.1"
                     /translation="MDLLITIGQLILGLSILVALHEFGHFLPAKLFGMRVEKFFLFFD
                     WPRHLWQKKVGDTTYGLGMLPFGGYVKIAGMIDESMDREQKERMERPPEPWEFRAKPV
                     WQRLIVMVGGVTVNVLLGFAIYSMVLYTWGSERMPLTNLTWGVTPNLIMEEQGFQSGD
                     RIVAVPGEHVETLEDVSLAILIRGARTVTVERNGARKDITLTDDIAEKILDTKDKQLF
                     AERFPFVVDSVMAGSGAQDAGGFRPGDRVMAVNGQPAAFFSDFRIALKGKEGRQVDVT
                     VRREGHLVVVVGDLGEEDDLLVGGPQRATIGDLEAQHIGVELDHAVHVVRMRGVVVDL
                     DGDVVPAVAL"
BASE COUNT          223 a          350 c          387 g          210 t
ORIGIN      
        1 cgttcatacc gcatccttcg ttggacgacc tggagcgcac cgacgccgaa gcgcgccgca
       61 ccgcccaaca cctgatcccc gcacccgcca catggatctc ctgatcacca tcggccagct
      121 catcctgggc ctctccatcc tggtggccct gcacgagttc ggccacttcc tgcccgcgaa
      181 gctcttcggc atgcgcgtgg agaagttctt cctcttcttc gactggccgc gccacctctg
      241 gcagaagaag gtgggcgaca cgacatacgg ccttggtatg ctcccctttg gcgggtatgt
      301 gaagatcgcg ggcatgatcg acgagagcat ggaccgcgag cagaaggagc gcatggagcg
      361 gccacccgaa ccttgggagt tccgcgccaa acccgtatgg caacgcctga tcgtcatggt
      421 gggcggtgtc accgtgaacg tgctgctcgg cttcgccatc tacagcatgg tgctgtacac
      481 ctgggggagc gagcgcatgc cacttaccaa cctcacctgg ggcgtcaccc ccaacctgat
      541 catggaggaa cagggcttcc agagcggcga ccgcatcgtg gccgtgccgg gagagcatgt
      601 ggagaccttg gaggacgtgt cgttggccat cctgatccgg ggcgcacgca ccgtgaccgt
      661 ggagcgcaac ggcgcccgga aggacatcac gcttacggac gatatcgctg aaaaaatact
      721 cgatacaaag gataagcaac tgttcgccga gcgctttccc ttcgtggtgg acagtgtgat
      781 ggcgggcagc ggtgcccagg atgccggcgg attccgcccg ggggatcgcg tgatggcggt
      841 gaacggacag cccgccgcct tcttcagcga cttccggatc gcgctcaagg gcaaggaggg
      901 tcggcaggtg gatgtgaccg tgcgacgtga aggccacctg gtggtggtgg ttggcgatct
      961 gggagaggaa gatgatctcc tggtcggtgg gccccagcgg gccacgatcg gtgacctcga
     1021 agcccagcac atcggagtag aactggatca tgcggtccac gtcgtaagga tgcgcggggt
     1081 ggtagtagac ctcgatggcg acgtcgttcc agcggtcgcg ctttaaggcg tagcgcgccg
     1141 agatgaacga atagaagttg aggatcttgg
//
LOCUS       DAOQOC010001160         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_115568_length_1170_cov_2.493274, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001160 DAOQOC010000000
VERSION     DAOQOC010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115568_length_1170_cov_2.493274"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1170)
                     /locus_tag="PL106_15205"
     CDS             complement(<1..>1170)
                     /locus_tag="PL106_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA34475.1"
                     /translation="GGLCRFDGHRFTTFTIGEGTGLAKGINCIQEDQQGVLWFGSRGH
                     GVFRYDGKDFTIMPVIDPEGRDLLLDMVLANDGSLWASTDHGLARYDGRMFRHVIPMN
                     GDSLDDVISMVADADGSLWLGRENGGITRCAIDHGRMMTTDIQLLPGEPVRVAQMING
                     RGGTLWIGTTTHGALLFNPREQGPSRVRRLSPAQGLPSDEVLCVVMGRHGDVWLGSRG
                     AGLTHYHGEALSTFRGIRPISMAEDPQGILWLGTTGGLARFDGQGFSEQRTGSGTPGW
                     VYSVSIDPLGRAGFGRNMIDPRVNGISWFDGARYHVTSVPDDRKFPDIFWTLHDHHGH
                     LWVGGRRGVERYADGQRTSYTTGQGLGSDLILSLAEDGHGSIWAGTDGGGVSRID"
BASE COUNT          225 a          396 c          334 g          215 t
ORIGIN      
        1 ctgtcgatgc gcgaaacgcc cccgccgtcc gtacctgccc aaatggatcc gtgcccgtcc
       61 tcggcgagtg agaggatcag gtcgctcccg agtccctgac ccgtggtgta actggtgcgc
      121 tggccatcgg cataacgttc cacgccccgt cgccccccga cccaaaggtg accgtggtgg
      181 tcatgaagcg tccagaagat gtcggggaat ttccggtcat cgggtacgct cgtcacgtgg
      241 taacgcgcac catcgaacca gctgatcccg ttcacacggg ggtcgatcat gttcctgccg
      301 aaacctgcac gtcccagggg atcgatactt accgagtaga cccaccccgg tgtaccggac
      361 ccggtgcgtt gttcactgaa gccttgccca tcaaaacgcg ccagcccgcc cgtcgtgccc
      421 agccacaata tgccttgcgg gtcctcggcc atgctgatcg gtctgatgcc gcggaaggtg
      481 gacagggctt caccgtggta gtgggtgagc ccggcacccc gtgatccgag ccagacatcg
      541 ccgtgcctgc ccatcaccac gcacaatacc tcgtccgatg gcaaaccctg cgcgggggac
      601 aatcggcgca cccgtgaagg accctgctcc cgtgggttga acaacagcgc accgtgcgtc
      661 gtggtcccga tccagagtgt gcctcctcgg ccgttgatca tttgagcgac acgaacaggt
      721 tcgccgggca ggagttggat atcggtggtc atcatgcgac catggtcgat cgcacaacgc
      781 gtgatgcctc cgttctccct cccgagccac agggatccat cggcgtcggc caccatggag
      841 atcacatcat ccagtgaatc tccattcatc gggatcacat gtcggaacat acggccatcg
      901 taccgcgcga gcccgtgatc ggtcgacgcc cagagcgagc cgtcattcgc caggaccatg
      961 tcgagcagga gatccctgcc ctcaggatcg atcaccggca tgatcgtgaa gtccttgccg
     1021 tcgtaccgga acacgccgtg gccacgggac ccgaaccata acacgccctg ctgatcctcc
     1081 tggatgcagt tgatgccttt ggccagacct gttccttcac cgatcgtgaa cgtggtgaaa
     1141 cgatgcccgt cgaagcggca aagtccaccc
//
LOCUS       DAOQOC010001161         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_115952_length_1168_cov_2.981132, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001161 DAOQOC010000000
VERSION     DAOQOC010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115952_length_1168_cov_2.981132"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PL106_15210"
     CDS             <1..416
                     /locus_tag="PL106_15210"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNA34476.1"
                     /translation="GQPAALDLRIDTLGHDAGGIDARIKVKLAQAPGSFTHALTLYLL
                     ESHIIDWQIDGNANPPDIPNYEHNHVLRSALNGTWGQNLDLTGLTTGDSLMVDLNDLA
                     VPSNVLDINNCYVLAYVYRTDTYEVLQVIQEPLHP"
     gene            579..794
                     /locus_tag="PL106_15215"
     CDS             579..794
                     /locus_tag="PL106_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34477.1"
                     /translation="MMDQGRKMMELSKQVLQKVSFDSRLFKKELLKARRWLGQHDQLL
                     LKAWCLATFGHQYKDIILEVFQKSMRS"
     gene            complement(926..>1168)
                     /locus_tag="PL106_15220"
     CDS             complement(926..>1168)
                     /locus_tag="PL106_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNA34478.1"
                     /translation="GRFEGWAFGCDICQQVCPWNRFSTPHNEPEFTPSAKLLDLTRDE
                     WHGMTEVLFEELFHGSAVKRTKFAGLKRNLGFLLTE"
BASE COUNT          236 a          391 c          337 g          204 t
ORIGIN      
        1 tcggtcagcc cgccgcgctg gacctgcgca tcgacaccct tggccacgac gccggcggca
       61 tcgatgcgcg catcaaggtg aagctggccc aggcaccggg ttcgttcacc cacgccctta
      121 cgctctacct gctcgaaagc cacatcatcg actggcagat cgacggcaac gccaacccgc
      181 ccgatatccc gaactacgag cacaaccacg tgctgcgcag cgccctcaac ggtacgtggg
      241 ggcagaacct ggacctcacc ggcctgacca ccggcgactc cctgatggtg gacctgaacg
      301 acctggccgt gccgtccaac gtgctggata tcaacaactg ctacgtgctg gcctatgtgt
      361 accgcacgga cacctacgag gtgctccagg tgatccagga gcccctgcac ccctaagagc
      421 cccatacgga tcggcacccg gcccacgttc catcccttca aggcggtgga tcgtgaaaaa
      481 acgtgaccac cggatcagcg ccgccgaggt tcggccgtat cttcagcctc gccttttctg
      541 acccccccgg ccgcccgggg ctaaaccgac gagatgtgat gatggaccag ggacggaaga
      601 tgatggaact gagcaagcaa gtgctccaga aggtgagctt cgacagccgc ctcttcaaga
      661 aggaactcct caaagcccgc cgatggttgg gccagcatga tcaactgctc ctgaaggcct
      721 ggtgcctggc cacgttcggg caccagtaca aggacatcat cctggaggtg ttccagaaga
      781 gcatgcggag ctgagcggcg gtgcgttgag ggttgtcggg cttcggtagt cggctgatgg
      841 ttgtctgttg gcaggcttcc gtttgatcgc tcctaccatc cagccggaca acaggcaacg
      901 gacaaccgat cactgacccg ctgaactact ccgtcagcag gaaacccagg ttccgcttca
      961 accccgcgaa cttcgtgcgc ttcacggcgc tgccgtggaa gagctcctcg aagagcactt
     1021 ccgtcatgcc gtgccattcg tcgcgcgtca agtccaacag cttcgccgat ggggtgaatt
     1081 ccggctcgtt gtgcggcgtg ctgaagcggt tccacgggca cacctgctgg cagatgtcac
     1141 aaccgaacgc ccagccttcg aagcggcc
//
LOCUS       DAOQOC010001162         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_115975_length_1168_cov_2.668464, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001162 DAOQOC010000000
VERSION     DAOQOC010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115975_length_1168_cov_2.668464"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PL106_15225"
     CDS             <1..1150
                     /locus_tag="PL106_15225"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34479.1"
                     /translation="PKPGDPDRIVMFHNSAEPFQSILSYAYVDPELENGSGSFTSGMS
                     PLTGEAARKLTAVAHANGQDYWVLSQLWGTDAIAAYSLDANGVDTMPVISHTGVPLSM
                     SVDGIEQALGWGSLVVSPAGDRLALVASLPNAIEPYDLEHAFVEILAFDPATGIAVHV
                     ATIPPPEGITIDGLEFSPDGSKLYGSTTSYVGSVHYDIHQYDLSILEEISIVASDTVV
                     FTGTFVGNFYDPDLQLALAPDGRIWFHTGMFSPWLGVIDAPNEAGMACMPIGEYLLLP
                     NNAGGGLPNQCKRYHDSDLNTGTTPEPVMGGLTVWPNPVRDVLYVNAPVEGSTSFLYD
                     ATGRLVRSGSAGQRRTAIMDVEGLPAGPYLLRCADEEGHSWLSKVVKE"
BASE COUNT          226 a          327 c          361 g          254 t
ORIGIN      
        1 gccaaaaccc ggtgatccgg atcgaattgt catgttccac aatagtgctg aaccattcca
       61 gtccatcctg tcatatgcgt acgtggatcc ggaattggag aacgggtcgg gatccttcac
      121 cagcgggatg agccctttga ccggagaggc cgctcgcaaa ctcacggccg tggcgcacgc
      181 caacgggcag gactattggg tgctttccca attgtggggc acggatgcga tcgctgccta
      241 ttcgcttgac gcgaatggcg tagataccat gccagtgatc agtcataccg gtgtgccgtt
      301 gtccatgtcg gtggacggta tagaacaagc gctcggctgg ggctctttgg tggtttcccc
      361 tgctggcgat agacttgcat tggtcgcgtc actgccgaac gccattgagc cttatgacct
      421 cgaacatgcg ttcgtggaga tcttggcttt cgatccggca acaggcatcg ccgttcatgt
      481 tgcgaccatc cccccacccg aaggcataac catcgatggt ctggagtttt caccggacgg
      541 tagcaaattg tacggatcaa ccacgtcata tgtgggcagc gtccattacg atatacatca
      601 gtacgacctg tcgattttgg aggagatcag catcgtggct tcggatactg tggtcttcac
      661 aggaaccttt gtgggcaact tttatgatcc ggatcttcaa ctggccttgg cgccggacgg
      721 gcgtatctgg ttccataccg ggatgttctc tccgtggctg ggcgtcattg acgcgccgaa
      781 tgaagccggc atggcctgca tgcccattgg ggagtacctg ctgctaccca acaacgccgg
      841 tggtgggctc cccaaccagt gcaaacgcta ccatgacagc gatctgaata ccggaacaac
      901 cccggagccc gtgatgggcg ggttgacggt ctggcccaac cccgtgcgcg acgtgttgta
      961 cgtgaacgca ccggtcgagg gcagcacatc gttcttgtat gatgccaccg ggcgcttggt
     1021 ccggtccggg tccgccgggc agcgccgaac ggcgatcatg gatgtggagg ggcttccggc
     1081 cgggccgtac ctgctgcgct gtgcggacga ggaaggccat agctggttga gcaaggtagt
     1141 gaaggaatag cgctgcccgc cgggaggc
//
LOCUS       DAOQOC010001163         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_116194_length_1167_cov_2.525180, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001163 DAOQOC010000000
VERSION     DAOQOC010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116194_length_1167_cov_2.525180"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PL106_15230"
     CDS             complement(<1..1139)
                     /locus_tag="PL106_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34480.1"
                     /translation="MALNLLGFITGVGSFMAIALLYFFRTNLDLYKMLGPDVERQGSI
                     QPMEDIMPMRPMPRQRQELRKATRWLRREQRTRKWTVETYLVRPWRLALARSSAHYDK
                     EILRSVLWQNHINGSIFSVVLVITFIALGAFSQAPLFAIPAGASVFMLFTIALMLLSA
                     LHSWLKGWTIAALLGIVLLLNVVSLHTDAFLADSQAVGLDYAGPAARYDRATIHAMAT
                     DTATARADADHFKGTLEQWLANNQALPNANGKPKLILVNTSGGGSRAMVWTFRCLQVA
                     DSLLGGDLMRRTALITGSSGGLIGATYYRQVEAAARKGGPQRTNDRSHLDALGSDILN
                     PLGFSFVTNDMFLRYRRVHDGTRSYTLDRGHAFDQRLNELTGGLL"
BASE COUNT          211 a          349 c          379 g          228 t
ORIGIN      
        1 tccaagagtc ctccggtcaa ttcattcagt cgttggtcga aggcgtggcc gcggtccagg
       61 gtgtagctgc gtgtaccatc gtgtacgcgc cggtatcgca ggaacatgtc gtttgtgacg
      121 aagctgaatc cgagcgggtt caggatgtcg ctgcccaagg cgtcaaggtg cgatcggtca
      181 tttgtccgtt gcggcccacc cttgcgcgct gcggcctcca cctgccggta ataggtggcg
      241 ccgatgagac cgccactgct gccggtgatc aatgccgtgc gccgcatgag gtcgcctccc
      301 agcagactat cggccacttg caggcagcgg aaggtccaca ccatggcgcg cgaacctccg
      361 ccgctggtgt tcaccaggat caatttgggt ttcccgttgg cgttgggcaa tgcctggttg
      421 ttcgccaacc attgctccag cgtgcctttg aaatgatccg catcggcccg agcggtggcg
      481 gtgtccgtgg ccattgcgtg aatggtggcc cgatcgtacc gggcggccgg tccggcatag
      541 tccagcccaa cggcctgcga atcggccagg aaagcatccg tgtgcaggct cacgacgttg
      601 aggagaagga cgatgcccag caacgccgcg atggtccatc ccttcagcca gctgtgcagc
      661 gcgctcaaca acatgagggc gatcgtgaac agcatgaata cgctggcccc ggcgggtatg
      721 gcgaagagcg gtgcctggct gaaggcgccg agggcgatga aggtgatcac caggaccacg
      781 ctgaaaatac tgccgttgat gtggttctgc caaagcaccg agcgcaggat ctccttgtcg
      841 tagtgcgcgc tgctgcgtgc cagggccagg cgccatgggc gcaccaggta tgtttccacc
      901 gtccacttcc tggtccgttg ctcacgacgc aaccagcggg tggccttgcg caattcctgc
      961 cgttgacggg gcatgggacg catcggcatg atgtcctcca tgggctgtat ggatccctgt
     1021 cgctcgacgt cgggcccaag catcttgtag agatccaggt tcgttcggaa gaagtagagc
     1081 agggcgatgg ccatgaacga tccgaccccg gtgatgaagc ccaacaggtt gagcgccatc
     1141 gcgcccacgg ggatcagctc cttgtgg
//
LOCUS       DAOQOC010001164         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_116733_length_1164_cov_3.429216, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001164 DAOQOC010000000
VERSION     DAOQOC010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116733_length_1164_cov_3.429216"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PL106_15235"
     CDS             complement(<1..1071)
                     /locus_tag="PL106_15235"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNA34481.1"
                     /translation="MKLLKDILYRVRIEQVIGSTNAAIEHIAFDSRDVRPLTAFVAIK
                     GGASDGHAFISKALDLGATAIICETMPEERKDGVTYVRVADSRRALATLADNFFDHPS
                     RDLRLTGITGTNGKTSVATLLFRLFRALGHKCGLISTVEITIGNTVLPSTHTTPDALN
                     LNALLRRMVDEGVTHCFMEVSSHSVMQERITDLEFDVAVFTNLTHDHLDYHGTFDAYL
                     NAKKRFFDLLGDGATALVNADDPNGAVMLQNTRASKRSYAVRNVADHRARIVENAITG
                     LHLNIDGHDVYTRLVGEFNASNLIAVYASALLLGKEPMDVLTALSGLEPPRGRFQIIR
                     ASGMPPRAQGRTTTGGDAPDADP"
BASE COUNT          189 a          338 c          410 g          227 t
ORIGIN      
        1 tggatcggca tccggggcat cgccacccgt ggtggtgcgg ccttgtgccc ggggcggcat
       61 gcccgaggcc ctgatgatct ggaatcgacc acggggcggc tccaggccgc tcagcgcggt
      121 aaggacatcc atgggctcct tgcccagcag cagggcgctg gcgtacaccg cgatcaggtt
      181 gctggcgttg aactcgccca cgagccgcgt gtaaacgtcg tgcccgtcga tgttgaggtg
      241 caacccggta atggcgttct caacgatacg ggcacggtga tcggccacgt tgcgtacggc
      301 gtagctgcgc ttgctggcac gggtgttctg gagcatcacc gcgccattcg gatcatcggc
      361 gttcaccagc gctgtggccc catcgccgag caggtcgaag aagcgtttct tggcgttcag
      421 gtaggcgtcg aacgtgccgt ggtaatcgag gtggtcgtgg gtgaggttgg tgaacacggc
      481 cacatcgaac tccaggtcgg tgatacgctc ctgcatcacg ctgtggctgc tcacctccat
      541 gaagcagtgc gtcacgccct catcgaccat gcgccggagc agggcgttca ggttcaacgc
      601 atccggtgtg gtgtgggtgg agggcagtac cgtgttgccg atggtgatct ccaccgtgct
      661 gatgagcccg catttgtgcc ccaacgcgcg gaagaggcgg aagagcaggg tggccacgct
      721 ggtcttgccg ttggtgcccg tgatgccggt gagtcgcagg tcgcgcgagg ggtgatcgaa
      781 gaagttgtcg gccagggtgg ccagtgcccg acggctgtcg gccacgcgca cgtaggtgac
      841 accatccttg cgttcctcgg gcatggtttc gcagatgatg gcagtggccc ccagatccag
      901 tgccttggaa atgaaggcgt ggccgtcgct tgccccgccc ttgatggcga cgaaggcggt
      961 gagcggacgg acatcgcgac tatcgaaggc gatgtgctcg atggcggcgt tggtgctgcc
     1021 gatcacctgt tcgatccgga cgcggtagag gatatccttc agcagcttca tgcgagctcg
     1081 atggtgatgg cggatccttt cacgaggcgc gatccgggca tgatcgactg ccgccggacc
     1141 atgccggtac cgctgatgcg cacg
//
LOCUS       DAOQOC010001165         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_117219_length_1162_cov_2.664860, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001165 DAOQOC010000000
VERSION     DAOQOC010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117219_length_1162_cov_2.664860"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1162)
                     /locus_tag="PL106_15240"
     CDS             complement(<1..>1162)
                     /locus_tag="PL106_15240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNA34482.1"
                     /translation="GISRAEALRQAHAPRDQHLLDTARHRLKFEELFFIQLGLLKQKV
                     LMARSVKGAVFGTVGDLFNTFYTKHIPFEPTGAQKRVVKEIRKDMGSGRQMNRLVQGD
                     VGSGKTLVALLSMLIALDNGYQCALMAPTEILAQQHFNTLTRMLEGMPLTVRLLTGGA
                     KSAERKALLTALRTGHIHILIGTHALLEERVVFQRLGLAVIDEQHRFGVAQRARLWAK
                     AELPPHVLVMTATPIPRTLAMTLYGDLDVSIIDELPPGRKPVRTVHRYDSSRSAVFGF
                     MEQELRKGRQVYVVYPLIEESVKGDPATNPAKDLKDLTDGYESLSRRFPLPKYAIGMV
                     HGRMEPADKDFEMARFKKGETDILVSTTVIEVGVDVPNASVMVIESAERFGLS"
BASE COUNT          196 a          375 c          360 g          231 t
ORIGIN      
        1 gtgaaagacc gaatcgctcc gcgctctcga tcaccatcac gctggcgttc ggcacgtcca
       61 cccctacctc gatcaccgtg gtgctcacca ggatgtccgt ctcccccttc ttgaagcgcg
      121 ccatctcgaa gtccttgtcg gccggctcca tacggccatg taccatgcca atggcatatt
      181 tcggcaacgg gaaacgacgg ctcaaactct cgtatccgtc ggtcaggtcc ttcaggtcct
      241 tggccgggtt ggtggccgga tcgcccttga cgctctcctc gatcaagggg tacaccacgt
      301 acacctgccg ccctttccgg agttcctgct ccatgaagcc gaacacggcg ctgcgcgacg
      361 agtcgtaacg atgaacggtc cgcacgggtt tgcggcccgg tgggagctca tcaatgatac
      421 ttacatccag gtcgccatac agcgtcatgg ccagtgtgcg cgggatgggc gttgcggtca
      481 tcaccaggac gtgcggcggc agttcggcct tggcccagag cctggcccgt tgcgccaccc
      541 cgaaacggtg ctgctcatcg atcaccgcca ggcccagccg ctggaacacc acacgctctt
      601 ccagcaatgc gtgggtgccg atcaggatgt ggatgtgccc ggtgcgcaac gcggtgagca
      661 gggccttgcg ctcggcggac ttcgcaccgc ccgtgagcag ccgcaccgtg aggggcatgc
      721 cctcgagcat gcgtgtcagg gtgttgaaat gctgctgtgc gaggatctcg gtgggtgcca
      781 tcaacgcgca ctggtagccg ttgtccaggg cgatgagcat gctgagaagg gccacgaggg
      841 tcttgccgct gcccacgtcg ccctgcacca ggcgattcat ctggcggccg ctgcccatgt
      901 ccttgcggat ctccttcacc acccgcttct gggcacccgt gggctcgaag gggatgtgct
      961 tcgtatagaa ggtgttgaac aggtcgccca ccgttccgaa gacggctccc ttcacgctcc
     1021 gggccatcag gaccttctgc ttcagcaggc ccagttggat aaaaaaaagt tcctcgaatt
     1081 tgagtcggtg acgcgcggtg tccagcaggt gctgatcgcg tggcgcgtgg gcctggcgga
     1141 gtgcttcggc acgggagatt cc
//
LOCUS       DAOQOC010001166         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_117258_length_1162_cov_2.324300, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001166 DAOQOC010000000
VERSION     DAOQOC010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117258_length_1162_cov_2.324300"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PL106_15245"
     CDS             complement(<1..371)
                     /locus_tag="PL106_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34483.1"
                     /translation="MPTTGEIDLEADYAITWDMGPASDGIQTIGDEFTVGTDAARVLA
                     ARAKYPYWIAYCTCDSAGLPPANRVPTPGAEQWTDAQILACSSFTAAWNDAVLKTSGA
                     IAGGVTFSNYPFSRANIIAPHG"
     gene            complement(374..>1162)
                     /locus_tag="PL106_15250"
     CDS             complement(374..>1162)
                     /locus_tag="PL106_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34484.1"
                     /translation="QGIQGNPGHTPVLTSSTAPPSGGTDGDWHIQQKNSGLWQVWARA
                     SGVWTALPSGTLQSGRILGSGATSPNLLPGLDPNANDLYFTLIGTNATFWQYTGATWV
                     AIVTWSTAGGGGGADTLSSAAANSPTGPSGQYMGTQVWNLQRSIQIVPGSTSYNVTGG
                     TFAADLGIAYHIVTLGPAFSTGGTINMSHTGLVSGIRGRWTFEITNPHAVWCGIGYAA
                     GKWSKNPGVTEPVSIDAGETLAISCEVINGRLNIVDVQKNYTNI"
BASE COUNT          218 a          351 c          369 g          224 t
ORIGIN      
        1 ccgtgcggag cgatgatgtt ggcgcggctg aacgggtagt tgctgaaggt caccccgcca
       61 gcgatggctc ccgatgtctt gagcacagca tcattccacg ccgccgtgaa ggatgaacac
      121 gccaggatct gggcatccgt ccactgttct gcgccgggtg tcggtacgcg gttggccggg
      181 ggtaacccag ccgaatcaca ggtacagtag gcgatccagt agggatactt ggcacgggcg
      241 gcaagcacac gggcggcatc agtacccacc gtgaactcat cgccgatggt ctgaatgccg
      301 tcgctggccg gccccatgtc ccatgtgatg gcgtaatcgg cctccagatc aatttcccca
      361 gtggtaggca tggtcagatg ttggtgtagt tcttctgcac gtccacgata ttgaggcgtc
      421 cgttgataac ctcgcatgag atagcgaggg tctctcctgc gtcaatcgaa acaggctcag
      481 tcacgccggg gttcttactc cacttgccag cggcgtaccc aatgccgcac cacacagcgt
      541 gcgggttggt gatctcgaag gtccaccgcc cacggatgcc cgagacgagg cccgtatggc
      601 tcatgttgat ggtaccgccc gtggagaacg ccggcccgag ggtcacgatg tgataggcaa
      661 tacccagatc agcagcgaag gttccgcccg ttacgttgta cgaagtggag ccgggaacga
      721 tctggatgga gcgttgcagg ttccacacct gcgtccccat gtactgaccc gaaggtccag
      781 tggggctatt ggcggcagca ctggagagcg tatctgcgcc gcccccacca ccggccgtgc
      841 tccacgtcac aatggccacc catgtagccc cggtgtactg ccagaacgtg gcgttcgttc
      901 cgatcagagt gaagtacagg tcattggcgt tggggtccag acccggcagc aggttcgggc
      961 ttgttgcacc ggaaccaagg atccttccgc tctgaagcgt tccgctcggc agggcggtcc
     1021 acacgcccga ggcgcgtgcc catacctgcc acagcccgga gttcttctgc tgaatatgcc
     1081 agtctccatc agtaccaccc gagggagggg cggtgctgga ggtgagcacg ggggtgtggc
     1141 cggggttgcc ctgaataccc tg
//
LOCUS       DAOQOC010001167         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_117990_length_1158_cov_4.388033, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001167 DAOQOC010000000
VERSION     DAOQOC010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117990_length_1158_cov_4.388033"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PL106_15255"
     CDS             complement(<1..241)
                     /locus_tag="PL106_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNA34485.1"
                     /translation="MDFLALDFETATSQPDSPCELGIAVVRGDVVREVRNWLIKPRCW
                     PHFSPWNVAVHGITARDVADAPGWEEVWEEASALIE"
     gene            331..>1158
                     /locus_tag="PL106_15260"
     CDS             331..>1158
                     /locus_tag="PL106_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNA34486.1"
                     /translation="MTHRILFLCALLSAGTVSAQPPTSSLIPLPVRVVPADGAFTFDV
                     ASTIALDQRELTDEGEFLRDGIRSLLGFAPMLLPAPKGRTVRLSILQRDSIPAEGYTL
                     AITPDRIDIIGADEAGVFHGITSLLNMMRQGDNASPGIACRTITDLPRFAWRGMHLDV
                     CRHFFPVAFVKRYIDLLARYKMNRFHWHLTDDQGWRIEIKKYPKLTQVGAWRNGSQYG
                     PYNQHTFDSTRYGGFYTQEQIRDVVAYAQARHITVVPEIEMPGHALAALAAYPELGCT
                     "
BASE COUNT          222 a          401 c          332 g          203 t
ORIGIN      
        1 cctcgatcag tgccgatgcc tcctcccaga cctcctccca tccgggggca tcggccacgt
       61 cccgggccgt gatgccatgt accgccacat tccatgggct gaaatgcggc cagcaacgcg
      121 gcttgatgag ccaattgcgc acctcgcgca ccacgtcgcc ccgtaccacg gcaatgccca
      181 actcgcacgg gctgtcgggt tgcgaagtgg cggtctcgaa gtcgagggcg aggaagtcca
      241 tgacggggta caaggtagcg cggatacgat ccgtgcaaca ggaggaaccc accatgcgat
      301 ggatccccgg acatcgcatc tttgacgggc atgacccatc gtattctttt cctctgcgcc
      361 ctcttgtcgg ccgggaccgt gagtgcgcag ccacccacaa gctcgttgat ccccttgccc
      421 gttcgggtgg tgccagccga cggcgcgttc acattcgatg ttgcatccac catcgcgttg
      481 gatcagcgcg aactcacgga cgagggcgaa ttcctgcgcg atggcatccg gtccctgctt
      541 ggcttcgcac ccatgctgct gccggcgccg aaaggcagga ccgtccggtt gtccatcctc
      601 cagcgcgaca gcatacctgc cgaagggtac acactggcga tcacccccga ccgtatcgac
      661 atcatcgggg ccgatgaggc aggcgtcttc cacggcatca cctccctgct caacatgatg
      721 cgccagggcg acaatgcatc gcccggcatc gcctgtcgca ccatcaccga tctcccgcgc
      781 ttcgcgtggc gtggcatgca cctggacgtg tgccgccact tcttccccgt ggcattcgtg
      841 aagagataca tcgacctgct cgcacgctac aagatgaacc gcttccactg gcacctgacg
      901 gacgatcagg gctggcgcat cgagataaaa aaatacccga agctcacgca ggtgggcgcc
      961 tggcggaacg gcagccagta cggaccgtac aaccagcaca ccttcgacag tacccgctat
     1021 ggtggtttct acacgcaaga acagatccgc gatgtggtgg cctacgccca ggcccggcac
     1081 atcacggtgg ttccggagat cgagatgcct gggcacgcac tggccgccct ggccgcctac
     1141 cccgaactcg gatgcacg
//
LOCUS       DAOQOC010001168         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_118301_length_1157_cov_2.704174, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001168 DAOQOC010000000
VERSION     DAOQOC010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118301_length_1157_cov_2.704174"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..>1157)
                     /locus_tag="PL106_15265"
     CDS             complement(6..>1157)
                     /locus_tag="PL106_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HNA34487.1"
                     /translation="LTSLLLICGTVLAQAGSLDASFNPTDPSGGASHGLNGGCQALVV
                     QPDGGILVGGSFTMVNGVERPHIARLNPNGSLDPSFAPGYGANDNVTAIALQSDGKIL
                     IGGAFTYYNGIWCNHLVRLHADGSVDSSFNTGLGFDGNVYALLVQPDNKILVGGDYFH
                     LDNQVAQGLVRLETNGARDTGFDAGGVPIVYALALRPNGRIIVGGNFSFVQGVERRCI
                     AALLPSGQLDPSFAPFGWNFSPVLGLDIQPDGRVLAVGMMPGCIARLELNGDMDPLLG
                     SGTGGFTGGPGVRVFGVDVDDQGRITCVGFFGAYNGVSRKGMTRLNPDGTLDESFSIG
                     EGFNAEVYALAVQPDGRYMVGGWFQEVDGTPRNRIARIMDQEVSTIGIV"
BASE COUNT          235 a          369 c          358 g          195 t
ORIGIN      
        1 gtgtgctaca cgataccgat ggtgctcacc tcctggtcca tgatgcgtgc gatgcggttg
       61 cgcggtgtgc catccacctc ctggaaccat ccgcccacca tgtagcggcc atcgggctga
      121 acggccagcg cgtacacctc ggcattgaag ccttcgccga tcgagaagct ctcgtccaaa
      181 gtcccatcgg ggttcagccg ggtcatgcct ttgcgcgaga cgccgttgta cgccccgaag
      241 aagcccacac aggtgatccg gccctggtcg tccacatcca caccgaacac acgcacgccg
      301 gggccgcccg taaagccacc tgtaccacta ccgagcaaag gatccatgtc gccattcagt
      361 tcgagtcggg cgatgcagcc gggcatcatg cccacggcca gtacgcgacc atcgggttgg
      421 atgtcgagac cgagcacggg gctgaagttc cagccgaagg gggcgaacga tgggtccaat
      481 tggccgctgg gcaacagggc cgcgatgcaa cgacgttcca cgccctgcac aaagctgaag
      541 ttgccaccta cgatgatccg gccgttgggg cgcaaggcca gggcgtacac gatgggcaca
      601 ccaccggcat cgaagcccgt atcgcgtgcg ccgttcgttt ccaagcgcac cagaccctgt
      661 gccacctggt tgtcgagatg gaaataatcg ccgcccacca ggatcttgtt gtcgggctgt
      721 accagcaagg cgtacacgtt gccatcgaag ccgagcccgg tgttgaagct gctgtccacc
      781 gagccgtcgg catggaggcg taccaagtgg ttgcaccaga tcccattgta ataagtgaac
      841 gcaccaccga tcaggatctt gccatcggat tgcaaggcga tcgccgtcac gttgtcattg
      901 gccccatagc ccggcgcgaa ggagggatcc aagctgccgt tggggttgag gcgggcgata
      961 tggggccgct ccacgccatt gaccatggtg aagctacccc ccaccaggat ccccccatcg
     1021 ggctgaacca caagggcctg gcagccgccg ttcaggccgt gggatgcgcc gccgctgggg
     1081 tcggttgggt tgaagctggc atcgagcgaa cccgcttggg cgagcacagt gccacaaata
     1141 aggagaagag acgtgag
//
LOCUS       DAOQOC010001169         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_118494_length_1156_cov_3.233424, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001169 DAOQOC010000000
VERSION     DAOQOC010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118494_length_1156_cov_3.233424"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PL106_15270"
     CDS             complement(<1..579)
                     /locus_tag="PL106_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34488.1"
                     /translation="MKLLNRSLLHVSMALLLVLALWAVAFFFILRGAVRHSIDEGLDD
                     QVEVITHRLKSDSSLLYVHELGLNGFAIAPAAAKEKERYTDTMLFVPSEGEVERVRVR
                     TGTFEHQGGHYRIQVYTSTVEEDDLVETMAWALIALYVLLLLTIVLVNNVVLRRLWQP
                     FQAMLAQLKSFRLGTSRELPPVRSRTSEFNELY"
     gene            complement(576..>1156)
                     /locus_tag="PL106_15275"
     CDS             complement(576..>1156)
                     /locus_tag="PL106_15275"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNA34489.1"
                     /translation="QVRFTDTISTLGATFTELGFQYNLGKHFAIGSDLRYYAMPLAHD
                     RARVAVNLHYKWRKNSFPLSVHYRMRFQHTTEPGTGKASSYLRNKLGLGYNLSHIVDP
                     LVEFESYFRFNGKNEFRRNRFTIGLEWRISKRIDLVTYYRIQNEFNKKEPERERIYAV
                     LLAYDLDLRRPQANPGPGKADIGARRIDARAR"
BASE COUNT          230 a          327 c          351 g          248 t
ORIGIN      
        1 gtacagctcg ttgaactcac tggtgcggct gcgcacggga ggtagttcgc gcgaggtgcc
       61 gaggcggaag gacttcagct gcgcgagcat ggcctggaag ggctgccaca gcctgcggag
      121 caccacgttg ttcaccagca cgatagtgag cagcagcaac acgtacaggg cgatgagcgc
      181 ccaggccatg gtctccacca ggtcatcctc ttccacggtg ctcgtataca cttggatgcg
      241 gtagtgacca ccttggtgct cgaaggtgcc ggtgcgcacc ctcacacgct ccacctcgcc
      301 ttcgctgggc acgaagagca tggtgtccgt gtagcgttcc ttctccttgg ctgcggcggg
      361 tgcgatggcg aagccattca ggccgagctc gtgtacatac aacaaggagc tgtcggactt
      421 caggcggtgg gtgatcacct ctacctggtc atccaaccct tcgtcgatgc tgtggcgcac
      481 ggccccgcgc aggatgaaga agaaggccac ggcccagagc gccagtacca gcagcagggc
      541 catggagacg tgcaggaggg aacggttgag cagtttcatc tcgccctcgc atcaattcgc
      601 cgtgcgccga tatctgcttt tccgggtccg gggttcgcct gcggcctgcg caggtccaga
      661 tcgtaagcaa ggagcactgc atagatccgt tcccgttcag gttccttttt gttgaactcg
      721 ttctggatcc ggtagtacgt gaccaggtcg atccgcttgg agatccgcca ttcaagtccg
      781 atggtgaagc ggttcctcct gaattcgttc ttgccattga acctgaaata ggactcgaac
      841 tccaccaagg ggtccacgat gtgggacagg ttgtatccca ggccgagctt gttcctcaaa
      901 taggaagatg cttttcccgt gcccggctca gtggtgtgct ggaatcgcat cctgtaatgt
      961 actgaaagcg ggaaactatt cttgcgccat ttatagtgca ggttcaccgc gacccttgcg
     1021 cgatcatgag caaggggcat ggcgtaatac cgaaggtcag atccaatggc gaagtgcttt
     1081 cccaggttat actggaaccc gagttccgtg aacgtggcac cgagcgtgga aatggtatcg
     1141 gtgaagcgca cctgct
//
LOCUS       DAOQOC010001170         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119103_length_1153_cov_3.653916, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001170 DAOQOC010000000
VERSION     DAOQOC010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119103_length_1153_cov_3.653916"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..>1153)
                     /locus_tag="PL106_15280"
     CDS             complement(472..>1153)
                     /locus_tag="PL106_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34490.1"
                     /translation="WGDYQSDPLGSAWASLGGGRSQVSGVVVTNPDFFRSWTLSSITD
                     PLPVELTAYNAACDGGKVKVTWTTASEIGTDRYVIEKSANAMEWAPIGEVAAAGNSTS
                     QITYTYYDEDVRGMAYYRLSQVDVNEQVNVLPVMVAGCDATNTEINNAWDDGALLNVV
                     VSSTDAYVYDLVLTDMQGRTILNAGSQAINPGYTTLRVNKNGIATGLYMVMLQNSQDV
                     MTRRVYLH"
BASE COUNT          235 a          347 c          328 g          243 t
ORIGIN      
        1 caacaagccg gtatcgtttc tgaatacatg ctcctcagcg ctttccagcc gttctgaaca
       61 cttctttgat cggaattgag gggagaaggg cagccacccg ataatgatac agatgtcctc
      121 gtccgtcaag gcctgcagcg cgggtcccac caggatccgg gacgagcgct gaatacagtg
      181 gtatggcgct tcggcgggct cagcgtgaca gttcaaaata gatcagcgtg agcgatcagt
      241 attatagcgc gctcctcgct tcgccgtagc ggctacgcag aggcgcagtc ggcaggctca
      301 gcgtagcagt tcaaatagat cagtgtgacc gttcggtacg atccttcagg aagcgttatc
      361 ccgaccaggt tgaagcgagg aacgggttga gcccgttaac cattcgtacg gtattaaaaa
      421 cggaagccct gccgttgagg caagggcttc caatgcgatc ctgatccgaa ctcagtgcag
      481 atacacccga cgcgtcatca cgtcctggga gttctgcagc atcaccatgt acaggccggt
      541 ggcgatgccg ttcttgttca cccgcaaggt ggtgtaaccc ggattgatgg cctgcgaacc
      601 cgcgttcagg atcgttcggc cctgcatgtc ggtgagcacg aggtcgtaca cgtacgcatc
      661 ggtggaactg accaccacat tgagcagtgc gccatcgtcc caagcattgt tgatctcggt
      721 attggtggca tcgcagcccg cgaccattac cggcagcacg ttcacctgct cgttcacatc
      781 cacctggctc aagcggtaat aggccatgcc gcgcacgtcc tcgtcgtagt aggtataagt
      841 gatctggctg gtgctgttgc ccgcagcggc cacttcgccg atcggtgccc attccatggc
      901 gttcgcactc ttctcgatca cgtagcgatc ggtgccgatc tccgatgcgg tggtccaggt
      961 caccttcacc ttgcccccat cgcaggccgc attgtatgcg gtcaactcaa ccggcagggg
     1021 atccgtgatg gatgagaggg tccacgaacg gaagaaatcc gggttcgtta ccaccacacc
     1081 gctcacctgg gagcgtcctc cacccaggct tgcccaagcg ctgcccagcg gatccgactg
     1141 gtagtcgccc cat
//
LOCUS       DAOQOC010001171         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119134_length_1153_cov_3.082878, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001171 DAOQOC010000000
VERSION     DAOQOC010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119134_length_1153_cov_3.082878"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..607)
                     /locus_tag="PL106_15285"
     CDS             complement(65..607)
                     /locus_tag="PL106_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA34491.1"
                     /translation="MNNRAFIKLPFLLTALLLTRPTHAQQMTNGTLEQRIARMEDRMA
                     IKHVVDVFSNLADVKQIDKQVLLFTDDAVVTSYAAGTLVSELKGRAQLLATFSGFLAG
                     FDVVYHQNGQQTIDELTDSTAKATSYCHVILVGMQDGKRMKTTMHTIYSDQFVKRDGV
                     WLIAKRRSDFVWREAVEVGE"
     gene            complement(604..>1153)
                     /locus_tag="PL106_15290"
     CDS             complement(604..>1153)
                     /locus_tag="PL106_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA34492.1"
                     /translation="EGKVGAFGLSEVAPDTIRKAHAVQPVAALQSEYSMLERVMEHGI
                     LDLCAELGIAFVPWGPLCRGMLTGRFGADYVPEPGLRRAGVPYFTPEALAANLRVVDL
                     ARQWATRKQATPGQVALAWLLAQAPHIIPIPGTTNEAHLLENIGALDVAFTGPELEEL
                     RATLQAIPTIGCRTPETLQQDQ"
BASE COUNT          188 a          350 c          378 g          237 t
ORIGIN      
        1 atggtgcggt catcatctgt gtccatctgt gtgcatccgt ggttgaatcg aattatgaaa
       61 aaggtcattc acccacctcc accgcttcgc gccacacgaa atccgaccgg cgcttggcga
      121 tgagccacac cccgtcgcgc ttcacgaact gatcgctgta gatcgtgtgc atggtggtct
      181 tcatgcgctt gccgtcctgc atgcccacca ggatcacatg gcagtacgag gtggccttcg
      241 ccgtgctgtc cgtgagttca tcgatggtct gctggccgtt ctggtggtac accacatcaa
      301 agcccgcgag gaagccgctg aaggtggcca gcaattgcgc ccggcctttc agttccgata
      361 ccagggtgcc tgcggcatac gaggtgacca cggcatcatc cgtgaagagc aacacctgct
      421 tgtcgatctg cttcacatcg gcgaggttgg agaagacgtc caccacgtgc ttgatcgcca
      481 tgcggtcttc cattcgggcg atgcgttgtt ccaaggttcc gttcgtcatc tgttgggcgt
      541 gtgttggtcg ggtgaggagg agggcggtga gcaggaaggg cagcttgatg aatgcgcggt
      601 tgttcattgg tcctgttgta aggtttcagg ggtgcggcag ccgatggtgg ggatcgcttg
      661 cagggtagcg cgcaactcct ccagttccgg tccggtgaac gcaacgtcca gtgccccgat
      721 gttctccagc aggtgtgctt cgttggtggt gccggggatc gggatgatgt ggggggcctg
      781 tgccagcaac cacgccaacg cgacctggcc cggcgtggcc tgcttgcgcg tggcccattg
      841 ccgcgccagg tccaccacgc gcaggttggc cgccagcgct tccggggtga agtagggcac
      901 acccgcccgg cgcagtccgg gttcgggcac gtagtccgcg ccgaagcggc cggtgagcat
      961 gccccggcac agcggacccc agggcacgaa ggcgatgccc agttccgcgc acaggtccag
     1021 gatgccgtgc tccatcacgc gctccagcat ggagtattcg ctttgcagcg cggccaccgg
     1081 ctgtaccgcg tgggctttac gaatggtgtc cggcgccacc tccgagaggc cgaaggcgcc
     1141 caccttgcct tcg
//
LOCUS       DAOQOC010001172         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119197_length_1153_cov_2.492714, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001172 DAOQOC010000000
VERSION     DAOQOC010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119197_length_1153_cov_2.492714"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PL106_15295"
     CDS             complement(<1..402)
                     /locus_tag="PL106_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNA34493.1"
                     /translation="MRPRLAYVNDSIGKVPPQAVDIEQALVGACLLGGGALRNVSGII
                     EAHQLYHDPTRKVWEVMIELDREGAAIDILTVTQRAAAKGILDEIGGAFFIAQLASRV
                     SSGANAEYHARVAQEKYIQRQLITLGHNLAAL"
     gene            complement(399..1115)
                     /locus_tag="PL106_15300"
     CDS             complement(399..1115)
                     /locus_tag="PL106_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34494.1"
                     /translation="MAKRFLDTSIFRDPSVRAMKAPYKVLYLYLLCECDHAGVWICEM
                     DVAALRLGMKLGGRDEVLAELAGAVIDLGGGAWYLVDFVRSQYGTLNPANRVHASVMD
                     RLQGLGMDPLNLPENKPLTSPLQGCKDKDKDKDNSEKKEVRRTAIAVPRATTPDEFRA
                     QCAAVAAEHPDLLPPSLRKGFLEYWTERDAQGRMRLQAEKHFEIPRRMNTWRTNAEKR
                     GEIPVPAPERKTGWLTPDGK"
BASE COUNT          195 a          363 c          342 g          253 t
ORIGIN      
        1 cagcgcggcc aggttgtgcc caagggttat cagttgccgt tggatgtact tctcttgggc
       61 gacacgggcg tggtactccg cgttggcgcc gctgctcacg cggctggcga gttgggcgat
      121 gaaaaaagcg ccgccgatct cgtcaaggat gcccttggcg gcggcgcgtt gtgtcaccgt
      181 gaggatgtct atcgcggcgc cttcgcggtc cagctctatc atgacctccc acaccttgcg
      241 ggtggggtcg tgatagagct ggtgggcctc gatgatcccg gacacgttgc gcagggcgcc
      301 cccgccaagc aggcaggccc caacaagcgc ctgctcgatg tccacggcct gcggcggcac
      361 cttgccgatg ctgtcgttga cgtatgcgag ccttggcctc atttgccgtc aggggttagc
      421 cacccggtct tgcgctcggg agcgggtacg gggatctcgc cgcgcttctc ggcgttggtc
      481 cgccaggtgt tcattcggcg ggggatctcg aagtgcttct cggcctggag gcgcatgcgg
      541 ccctgcgcat cgcgctcggt ccagtactcg aggaatccct ttcgcagcga tggcggcagc
      601 aggtcagggt gctcggcagc caccgccgcg cattgggccc tgaactcgtc cggcgttgtc
      661 gcgcgcggaa ctgcgatagc agttcttctt acttctttct tctctgagtt atccttatcc
      721 ttatccttat ccttacaccc ttgtaagggg cttgtaaggg gcttattttc gggaaggttc
      781 aaaggatcca ttcccaaacc ttgaagcctg tccatcacac ttgcatggac cctgttcgcg
      841 gggttcagtg tcccgtattg gctgcgcaca aagtccacca ggtaccacgc accacctccc
      901 aagtcgatca ccgctccggc caattccgcc agcacttcgt cacggccacc cagcttcatt
      961 ccaagcctca gcgccgctac gtccatctca cagatccaca cccccgcatg gtcgcactca
     1021 cataggagat acaaatacag gactttgtaa ggggctttca tggcccttac ggaagggtca
     1081 cggaagatgc tggtatcaag gaagcgcttg gccatagcag aaagggacgg cccccatgtt
     1141 ctccgttgac agg
//
LOCUS       DAOQOC010001173         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119884_length_1150_cov_2.118721, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001173 DAOQOC010000000
VERSION     DAOQOC010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119884_length_1150_cov_2.118721"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PL106_15305"
     CDS             complement(<1..354)
                     /locus_tag="PL106_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNA34495.1"
                     /translation="MIVQNSGSSLAQLSDEQPVLLLFLRQFGCSFCREAISDISKRRR
                     RLEAQGVRIVLVHMAQEIETAEKFFRKYKLYPIDHIQDPEKRFYQAFGLGRGTPVQLF
                     GLMNWIRGFQAGVIEG"
     gene            complement(412..>1150)
                     /locus_tag="PL106_15310"
     CDS             complement(412..>1150)
                     /locus_tag="PL106_15310"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNA34496.1"
                     /translation="LTAGSDIVVITAGIPRKPGMSRDDLISTNAKIVVSVTQNILKYT
                     KNPIIIVVSNPLDIMTYACWKASGLSDKRVLGMAGVLDTARYRAFLAEELDVSPKDIQ
                     AMLMGGHGDTMVPLPRYTTVSGIPVTEMIKKDKLDAIIERTKFGGGELVNLMGTSAWY
                     APGAAAAQMAVSILKNENRIYPCCVRMNGQYKLKDIFVGAPVKLGKKGIQEIIKLRLK
                     KDEMELLHKSADAVTEIVSALDKMNLI"
BASE COUNT          232 a          302 c          332 g          284 t
ORIGIN      
        1 gccttcgatc acgccggcct gaaaaccgcg tatccagttc atcaggccga acagttggac
       61 tggcgtaccg cgtcccaggc cgaaggcctg gtagaagcgt ttttccgggt cctggatatg
      121 atcgatcggg taaagtttgt atttacgaaa gaatttttct gctgtttcaa tctcctgcgc
      181 catatgcacg agcacgatcc ggacgccttg cgcctccagt cggcggcggc gtttggagat
      241 gtcggaaatg gcttcccggc aaaacgagca accgaattgg cgcaggaaca ggagcagcac
      301 cggttgttcg tcgctaagtt gcgccaggga gctgccactg ttctgaacga tcatggcttc
      361 ccaggtagag ggagccaggg tattgcgaag ggtttccgcc atagaatttt tttagatcaa
      421 attcatcttg tccagggcgc tcacaatttc cgtcacggca tccgcagatt tgtgcaacag
      481 ctccatttcg tcttttttga gtcgcaattt gataatttcc tggatacctt ttttcccgag
      541 tttgacggga gcgcccacaa aaatgtcttt caacttgtac tggccgttca tccgaacaca
      601 gcaagggtag atccggtttt catttttcag gatagaaacc gccatttgtg cagcagcagc
      661 gccgggcgca taccaggcgg atgtgcccat gaggttgacc aattcgccgc cgccaaattt
      721 ggtgcgttcg atgatggcat ccagtttatc tttcttgatc atctccgtga ccggtatgcc
      781 gcttacggtg gtgtagcggg gcaagggcac catggtatct ccgtgaccgc ccatgagcat
      841 agcctggatg tctttgggtg acacgtccag ttcttcggcc aggaaagcgc ggtagcgggc
      901 agtatccagc acgccagcca tgcccagcac gcgcttgtca gagaggccgg aggccttcca
      961 gcaggcgtag gtcatgatat cgagggggtt gctgaccacg atgatgatgg ggttcttcgt
     1021 gtacttgagg atgttctggg tgaccgacac cacgatcttc gcgttggtgc tgatgaggtc
     1081 gtcgcggctc atgccgggct tgcgcgggat gcctgctgtg atgaccacaa tatccgaacc
     1141 tgccgtcagg
//
LOCUS       DAOQOC010001174         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119898_length_1150_cov_1.947945, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001174 DAOQOC010000000
VERSION     DAOQOC010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119898_length_1150_cov_1.947945"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PL106_15315"
     CDS             <1..331
                     /locus_tag="PL106_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34497.1"
                     /translation="TDAMDTVAGFPCHKAVAIFDAIDQPEVELWYTDAIRMDKPNWYG
                     PYSEIPGVLLRYEMVQHGMRMRLNAVSVKPGPVDPAVFAPKADHDRVAPDVLHKELSD
                     VLSTFTN"
     gene            468..>1150
                     /locus_tag="PL106_15320"
     CDS             468..>1150
                     /locus_tag="PL106_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNA34498.1"
                     /translation="MAKPNTLREPELDEPVDHEAPTPKERKPRASGTRRKKDAEPGRF
                     DRLKAFLADERTHKAGGLLLVLMAIYLLVAFTSYLITWRIDQDLMARPWGEIFSTDTH
                     VENWLGKLGALVGHQFIYKWFGIAAFVVVLWSFLAGVRTVLGSWLLPRNRTFRWSLMT
                     MFWVPALLGFIFAHGELMFLGGGIGHAINAQLTSWLGRFGAGALILFSAASFMVYTFN
                     PSFAVLNGLL"
BASE COUNT          212 a          363 c          354 g          221 t
ORIGIN      
        1 caccgacgcc atggataccg tggccgggtt cccgtgccac aaggcggtgg ccattttcga
       61 cgcgatcgac cagcccgagg tggaactctg gtacaccgat gccatccgca tggacaaacc
      121 gaactggtac gggccgtaca gcgagatccc cggtgttctg ttgcggtacg agatggtaca
      181 gcacggcatg cgcatgcggc tgaacgccgt atcggtgaaa cccggacccg tggacccggc
      241 tgtattcgct cccaaggccg accatgaccg ggttgcaccc gacgtgctcc acaaggaact
      301 ttccgatgtg ttgagcacgt tcacgaactg agcgatcgga ccggcatacg gccgccccgc
      361 acagcggctt gttcataata gcggcccaac acggcacgtt ccctcctgtg ttgcccggaa
      421 tttcgctggg tactccgtcc cccgggacga aaaaccccga aatcgccttg gccaagccga
      481 atacgctccg cgaaccggaa ctggatgagc ccgttgacca cgaagcaccc acacccaagg
      541 agcggaagcc gcgtgcgagc ggcacacgtc ggaagaagga tgccgaaccc ggtcgattcg
      601 accggttgaa ggcgttcctg gccgatgaac gcacgcacaa ggccggagga cttttgttgg
      661 tactgatggc gatctacctg ctggtggcct tcacgagcta cctgatcacc tggcggatcg
      721 atcaggacct gatggcgcga ccgtggggcg agatcttcag caccgacacg cacgtggaga
      781 actggctggg caagctcggc gcactggtag gccatcaatt catctacaaa tggttcggca
      841 tcgcagcgtt cgtagtggtg ctctggagct tcctcgcggg cgtgcgcacc gtactgggca
      901 gctggctgct gccgcgcaac cggaccttcc gatggtcgct catgaccatg ttctgggtgc
      961 ccgcgttgct gggtttcatc ttcgcccatg gcgagctcat gttcctcggt ggcggcatcg
     1021 gccacgccat caacgcgcag ttgaccagtt ggctggggcg cttcggcgcg ggcgcgctga
     1081 tcctgttcag tgcggcgtcc ttcatggtgt acacgttcaa tccttccttc gcggtcctca
     1141 acgggttgct
//
LOCUS       DAOQOC010001175         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119901_length_1150_cov_1.920548, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001175 DAOQOC010000000
VERSION     DAOQOC010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119901_length_1150_cov_1.920548"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..285
                     /locus_tag="PL106_15325"
     CDS             52..285
                     /locus_tag="PL106_15325"
                     /inference="COORDINATES: protein motif:HMM:NF020588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HNA34499.1"
                     /translation="MRGCGGSKADRGALPADPNGDGGTDDGAAIKYQTTQMAQELVQL
                     VVPTAVFPTFTDAQRSRMLNVGGFIELVRSRQA"
     gene            complement(496..990)
                     /locus_tag="PL106_15330"
     CDS             complement(496..990)
                     /locus_tag="PL106_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34500.1"
                     /translation="MRIVVTDANIFFDLIGIGALAFFFQLEHEVHTTVLVIDECDPAD
                     LAQLQAYIDNGKLQVRAFNEDEMKVVETTGRRKGLRPADRSILLHALEMKGWVLSGDK
                     DVRNECGEMKLECHGMLWCLRELLRNGHYDKSGCLSALEQLEAINKWLPGKDVEKMRK
                     ELGG"
BASE COUNT          240 a          343 c          333 g          234 t
ORIGIN      
        1 agccacctat ctcgaaaccg gtgaagcacc ggacgggttc ttgatcgagg agtgcgagga
       61 tgcggaggaa gcaaggcgga tcgcggagca ctaccagcgg atcctaacgg cgatggtgga
      121 acagatgatg gagcagcgat aaagtaccag accacgcaga tggcccaaga gctcgtacaa
      181 ttggtggtgc ccaccgcggt attccccacc ttcaccgacg cgcagcgcag ccgaatgttg
      241 aacgtaggtg gcttcatcga actggtccgc agcaggcagg cgtgatggcc aagcgtacgg
      301 gcgtcttcac caaggccaaa tgctccgaag tggtgtcgaa gatccggggc aagggcacgg
      361 aagccgtgca gttcatcctg agcggctcca tggaagagtc ctgcgcgatc gcgccaacta
      421 ggcatggggc gtggtcgatg agagcggaca gcgtgatcag tcggtgcgcg tgtgtatgga
      481 acgcagcgtt gtggcctatc cacccagttc cttcctcatc ttctccacat cctttccggg
      541 cagccacttg ttgatcgcct ccagctgttc gagcgcggac aagcaaccgg acttgtcgta
      601 gtgcccattg cgcagcaact cgcgcaggca ccacagcatg ccgtgacact ccagcttcat
      661 ttctccgcat tcattccgaa catccttgtc accgctcagc acccagccct tcatttccaa
      721 tgcatgcaat agaatgctcc tgtcggccgg tctcaatccc ttgcggcgac ccgttgtctc
      781 caccaccttc atttcatcct cgttgaaggc gcgtacctga agcttgccgt tgtcaatgta
      841 tgcctgtagc tgggccaggt cggccgggtc gcactcgtcg atgacaagca ccgtggtgtg
      901 tacctcgtgc tccagctgaa agaagaaggc gagcgccccg atgccgatca ggtcgaagaa
      961 gatgttggca tcggtgacga cgatcctcat cgctggtccg actccaggat atagcggcgg
     1021 aacaagccca tcttcatgtt catgaggctt gcagcttgac cgagcgagag cgcttcctgg
     1081 gccagggcat gcaacagcat ttgcttgaaa cgcgccgtgc gttccttgta cacgaactca
     1141 ccgggttctt
//
LOCUS       DAOQOC010001176         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_119994_length_1149_cov_3.771481, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001176 DAOQOC010000000
VERSION     DAOQOC010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119994_length_1149_cov_3.771481"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..>1149)
                     /locus_tag="PL106_15335"
     CDS             complement(197..>1149)
                     /locus_tag="PL106_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34501.1"
                     /translation="LTQVYGTDAIAAYRLHAGGLDAVPVISHTGVPMTVVIDGIEQAQ
                     NEGIMAASRAGDRLAMAGIVSQSIEAHDLAHSRFEILHFDNAAGIATHLASIPLPVGS
                     TIDGIEFSPDGTKLYFTSTAAQQIPFTFTIHQYNVTAGDEQAIIASHTEVLSLEGMSN
                     SYTPDIQLALAPDGRIWFDHWTDSPWLGVIDAPNEAGMACTPIGEYLLLPNNAGVGLP
                     NQCKRYHDSDLNTGAAPEPVMGGLTAWPNPVYDELYINAPRMGCTSWLYDATGRVVRS
                     ASSGPRRTTIIGVAGLPSGPYLLRCTDGQGKSWTSTVLKE"
BASE COUNT          220 a          334 c          348 g          247 t
ORIGIN      
        1 gatcgttcgt gtttcatagc aaaggtaatg caccgatcgc acgttctgcc gcatctattg
       61 catatcggag gttcgaatgc agaatgtcaa tgttttggat gcgagtgcga atgcagatag
      121 cacctgtgtt cggaaagtcg tgtgctcatg ccttatcctt cggacctcgc gtagccaagc
      181 cttccaccgc gttgaactac tctttcaaca ccgtgctcgt ccagctcttg ccctgcccat
      241 cggtacagcg cagtaggtag ggcccggagg gaagtcctgc cacaccgatg atcgttgttc
      301 tgcgtggccc ggaagaagct gaccgcacca cgcggccggt cgcatcatac agccacgacg
      361 tgcagcccat acgtggcgcg ttgatgtaca actcatcgta cacgggattg ggccaagcgg
      421 tcaacccgcc catcacgggc tccggggccg ctcccgtatt caggtcgctg tcgtggtagc
      481 gtttgcactg gttgggcagc ccaacgccgg cattgttggg cagtagcagg tattccccaa
      541 tgggcgtgca ggccatgcct gcttcattcg gagcatcgat cacccccagc cacggggaat
      601 ccgtccagtg gtcgaaccag atgcgaccat ccggtgcgag cgccaactgg atatcggggg
      661 tataagagtt gctcatgcct tccaggctca gcacctcggt atgtgaagca atgatggcct
      721 gctcgtcccc tgccgtcacg ttgtactggt ggatggtgaa tgtgaacggt atctgctgtg
      781 cggccgtgct cgtgaagtag agtttcgttc catcaggcga gaactcgatg ccatcgatcg
      841 tggaaccgac cggtagcggt atggatgcca gatgggtcgc gatgcccgcc gcattgtcaa
      901 agtggaggat ctcgaagcgg gagtgagcga gatcgtgggc ctcgatgctc tgggaaacga
      961 tccccgccat ggccaggcgg tcccctgcgc gggatgccgc catgatccct tcgttctggg
     1021 cttgttcgat accatcaatg accacggtca tcggtacgcc ggtatgactg atcaccggta
     1081 cggcatccaa cccgccggcg tgcagccgat aggcggcgat ggcatcggtg ccgtacacct
     1141 gggtaagca
//
LOCUS       DAOQOC010001177         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_120354_length_1148_cov_2.175663, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001177 DAOQOC010000000
VERSION     DAOQOC010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120354_length_1148_cov_2.175663"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1148)
                     /locus_tag="PL106_15340"
     CDS             complement(<1..>1148)
                     /locus_tag="PL106_15340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA34502.1"
                     /translation="KRDKLFAALNILGLAIGITACFLIYIYVQDELSFDAHHAKADRI
                     YRVQAHYTFGDTQDDFGITPFPVVEALLKEYPDIEQGVSLFQLGQTTLTYQGKPYIAQ
                     DGYNADTSTFRVFDFTFTHGGPDALDEPDNIVIMQEMAVRMFGADDPIGKLVERNGRT
                     LKVAGVIDGKADNTHIPMGVFMSRLGMPPQAKEQLGQSWGNNSCFNYLVLAPGVDAQA
                     FQGKMDAFVTKYIIPQWGGDQFQGSIRFNLEPLREVHFNNDLIYDTPKKGNKAYVTLF
                     AIVAVLILAIACINYINMSTADATRRAKEVALRKVSGAQRGQLVAQFIGGSVLIAVIG
                     IAVALGLLWLALPAFNGITGKEIGMGYLLRGSFAGVVLLIVLAIGVLAG"
BASE COUNT          205 a          368 c          349 g          226 t
ORIGIN      
        1 cccgccagca cgccgatcgc cagcacgatc agcagcacca cccctgcaaa gcttccgcgc
       61 agcaggtagc ccatgccgat ctccttgccg gtgatcccgt tgaacgccgg cagcgccagc
      121 caaagcaggc ccaaggccac cgcgatgccg atcaccgcga tcagcacgct gccgccgatg
      181 aactgtgcca ccagttgccc gcgctgcgca ccactcacct tccgcaaggc cacctccttc
      241 gcgcggcgtg tggcatcggc cgtactcatg ttgatgtagt tgatgcaggc gatggccagg
      301 atgagcaccg ccacgatggc gaagagcgtg acgtaggcct tgttgccctt cttcggcgtg
      361 tcgtagatga ggtcgttgtt gaagtggact tcgcgcagcg gctccaggtt gaagcggatg
      421 ctcccttgga actggtcgcc accccattgc ggaatgatgt acttggtgac gaaggcgtcc
      481 atcttgcctt ggaaggcctg cgcatccacg cccggggcca gcaccaggta gttgaagcag
      541 ctgttgttgc cccagctctg ccccagctgc tccttcgcct gcggcggcat gcccaggcgg
      601 ctcatgaaca cccccatggg gatgtgcgtg ttgtcggcct tgccatcgat cacgcccgcc
      661 actttcagcg tgcgtccgtt gcgttccacg agcttgccga tcggatcgtc cgcaccgaac
      721 atgcgcacgg ccatctcctg catgatcacg atgttgtccg gctcatccag cgcatcgggc
      781 ccgccgtgcg tgaaggtgaa gtcgaagacc cggaaggtgc tcgtatccgc gttgtagccg
      841 tcctgcgcga tgtacggctt gccctgatag gtcaaggtgg tctgccccaa ttggaagagc
      901 gacacgcctt gttcgatgtc aggatactcc ttcaacaagg cctccacgac ggggaatggc
      961 gtgatgccga aatcgtcctg cgtatcgccg aaggtgtagt gggcctgcac ccggtatatg
     1021 cggtccgcct tcgcatggtg ggcatcgaag ctcagctcgt cctgcacata gatgtagatg
     1081 aggaaacagg ccgtgatacc gatggccagg ccgaggatgt tgagcgcggc gaagagcttg
     1141 tcgcgctt
//
LOCUS       DAOQOC010001178         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_120452_length_1147_cov_3.910256, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001178 DAOQOC010000000
VERSION     DAOQOC010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120452_length_1147_cov_3.910256"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PL106_15345"
     CDS             <1..1042
                     /locus_tag="PL106_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNA34503.1"
                     /translation="DFSVFTDLLRRAALDPTVTRISITLYRVARASLVCEALLKAVRA
                     GKEVTAFVEVQARFDEGNNLFWGEALEQAGARVIYGREGLKVHCKLCLIERREGRSAT
                     AYCWLGTGNFNERTARLYGDMGLLTANAAIARDVRNVFAHLHDQREPLRSKQLMVAPQ
                     GLRSGLDRLIDREIANALSGQEASILLKMNSLEDRPLIRKLYDASRSGVRIRLIVRGI
                     CCLVPGIPGLSENIEVISIVDRFLEHARAYVFHNSGDPLVFLSSADLMERNMDRRVEV
                     AFPVIDPELRMEVIDFLELQWRDQAKARTIDAAQRNHYRDPHWSGPQAIRSQLAFYEQ
                     LAASAKKKRSAK"
BASE COUNT          219 a          384 c          348 g          196 t
ORIGIN      
        1 cgatttcagc gtattcacgg acctgctccg gcgcgccgcc cttgacccca ccgtcacccg
       61 catctccatc accctgtacc gggtggcccg cgcatcactg gtgtgcgagg ccttgttgaa
      121 agccgtgcgc gcgggcaagg aggtcaccgc gttcgtggaa gtgcaggcgc gtttcgacga
      181 ggggaacaac ctcttctggg gcgaagcgct ggaacaggcc ggggcccgcg tgatctacgg
      241 ccgcgaaggc ctgaaggtcc attgcaaact ctgcctcatc gaacggcgcg agggacgatc
      301 ggcaacagcc tactgctggc tcggtaccgg caacttcaac gagaggaccg caaggctgta
      361 cggcgacatg ggcctgctga ccgccaacgc ggccatcgcc cgtgacgtgc gcaatgtgtt
      421 cgcccacctg cacgaccagc gcgaacccct gaggtcgaaa caactgatgg tcgccccaca
      481 gggcctgcgc agtgggctgg accgcctgat cgaccgcgag atcgcgaacg ccctgtccgg
      541 ccaggaagcc agcatcctcc tgaaaatgaa cagcctggag gaccgcccgt tgatccgcaa
      601 attgtatgac gccagccgct ccggggtgcg catccgcctg atcgtgcgcg gcatctgctg
      661 ccttgtgccc ggcatacccg ggctcagcga gaatatcgag gtcatcagta tcgttgaccg
      721 cttcctggaa catgcccggg cctatgtctt ccataacagc ggggatccgt tggtcttcct
      781 gtcctcggcc gacctgatgg agcgcaacat ggaccgacgt gttgaggtgg ccttcccggt
      841 gatcgatccc gaactgcgga tggaggtgat cgacttcctg gaacttcaat ggcgcgacca
      901 ggccaaggca cgtacgatcg atgcggcaca acgcaaccac tatcgcgatc cgcactggtc
      961 cggtccgcag gccattcgct cgcaactggc gttctacgaa caactggcgg cctccgcgaa
     1021 aaagaagcgc tccgccaagt gaccttcccg caccccgtgc gggacgcact ggccagctcc
     1081 catgcgaaag gcggcccttg cgaaccgcct tccttcgtgg agaataccgg agtcgaaccg
     1141 gtgacct
//
LOCUS       DAOQOC010001179         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_120460_length_1147_cov_3.636447, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001179 DAOQOC010000000
VERSION     DAOQOC010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120460_length_1147_cov_3.636447"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="PL106_15350"
     CDS             complement(<1..>1147)
                     /locus_tag="PL106_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNA34504.1"
                     /translation="GSAGSITIEVDNDAGGPNGYVAMLTVAAPGNYGPYGPYATGSPV
                     NVRLVHNVNALCNLNFNGVVGNCDGPGGLCAYSSSGATNILDNQTVTNTITVPPNGGQ
                     TLTDLNVYVNITHTYTSDLRLTLQSPSGTSVALINTGVCGSNDNMTVEFDQQAASTIG
                     TVCPMTGIFAIPSASLASFNGQAIQGTWTLSVQDVAGGDEGTINSWCLIPALSAPAVQ
                     VSPKVFLEGPYSTANGLMSDAMRSAALVPNAQPYTGLGYGFTGSPGAGGTLGGGVLAV
                     TGSNAIVDWVIVELRSTATPTTVVASCAALVQRDGDVVALDGTSPVSIAVASGAYHVA
                     IRHRNHLGVMTQNGVALSNTPSTVDFTSVATSTYGTNARKSMAGAFPA"
BASE COUNT          201 a          363 c          358 g          225 t
ORIGIN      
        1 gtgcggggaa tgccccggcc atgctcttgc gcgcgttggt gccgtatgtg ctcgtggcca
       61 ccgaggtgaa gtccaccgtg gagggcgtat tgctcagtgc cactccgttc tgcgtcatca
      121 cgcccagatg attgcggtgg cggatggcca cgtgataagc gccggaggct accgcaatgc
      181 ttaccgggga tgtaccgtcc agcgctacca catcaccatc gcgttgcacc agtgcggcac
      241 aactggccac caccgtggtc ggcgtggccg tactgcgcag ttctacgatg acccagtcca
      301 caatggcgtt gctgcccgtt accgccagca cccccccgcc caacgttcca cccgcgcccg
      361 gagagccggt gaaaccgtac ccgagaccgg tgtagggctg ggcattcggt accagcgcag
      421 cgcttcgcat cgcgtcgctc atgaggccgt tggccgtact atagggacct tccaggaaca
      481 ccttgggcga tacctgcaca gcgggagcgg acagtgcggg gatcaagcac caactgttga
      541 tcgtgccctc atcaccgcca gccacatcct gaacactcag cgtccatgta ccttggatcg
      601 cctgtccgtt gaacgacgcc aacgacgccg acgggatggc gaaaatgccg gtcatcggac
      661 ataccgtacc gatcgtggac gcggcttgtt gatcgaactc cacggtcatg ttgtcgttgg
      721 aaccgcacac acccgtgttg atgagcgcca cggacgttcc tgatggactc tgcagggtga
      781 gccggaggtc cgatgtgtag gtgtgcgtga tgttcacgta aacgttcagg tcggtaaggg
      841 tctgtccgcc gttgggcggc acggtgatgg tgttggtcac cgtctggttg tccaggatgt
      901 tcgtggcacc ggagctgctg tacgcgcaca gtccgcccgg accatcgcaa ttgcccacca
      961 cgccgttgaa gttcaggttg cagagcgcgt tcacgttgtg tacgagccgc acgttcaccg
     1021 gcgaaccggt cgcataaggc ccgtaaggtc cgtagttgcc cggtgccgcc acggtcaaca
     1081 tggccacgta cccgttgggg ccgccggcgt cgttgtcaac ctcgatcgtg atgctgccgg
     1141 cactgcc
//
LOCUS       DAOQOC010001180         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_120472_length_1147_cov_3.311355, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001180 DAOQOC010000000
VERSION     DAOQOC010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120472_length_1147_cov_3.311355"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PL106_15355"
     CDS             complement(<1..480)
                     /locus_tag="PL106_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNA34505.1"
                     /translation="MNTGTNDISTWPVRLGIRSGETILLMADLTRMAWNTRRSGKRFR
                     AADLLDAFADATGPDGTLLVPTYNYDLDDGAAFDVRRTRSISGALANAAIDHPRFLRT
                     PHPLHSFAVCGTGTEELIHTAERSSFGPRSPFAFLLAHKASLIVIDLPLNDALTFAHF
                     "
     gene            complement(477..953)
                     /locus_tag="PL106_15360"
     CDS             complement(477..953)
                     /locus_tag="PL106_15360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA34506.1"
                     /translation="MSNFTVRAVQRADDPALAKVPALFGTMHAEMAEQGMGQRLAPDG
                     GRTWLDGVIGGLERFGRIAVAEWDGEVIGFAHAAVKLAPEHLGGGRIGHVTHLYVTPA
                     HRRSGIARALAASLHEWLRTRSVLSIELQVVHRNEAGLAFWRSLGYAPELLQMRKH"
     gene            complement(950..>1147)
                     /locus_tag="PL106_15365"
     CDS             complement(950..>1147)
                     /locus_tag="PL106_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNA34507.1"
                     /translation="RLGLTPDDLTDDLDLVRSGILDSLSFVDLITDLEGATGKQVDLE
                     RALGHRKATTVAGVISMFVDA"
BASE COUNT          218 a          357 c          363 g          209 t
ORIGIN      
        1 aaagtgcgcg aaggtgagcg catcattcag cgggagatcg atgacgatca gggaggcctt
       61 gtgcgcgagc aggaaggcga agggggatcg cggaccgaag ctgctccgct ccgcagtgtg
      121 gatcaattct tccgttcccg tgccgcacac cgcgaatgaa tgcaacgggt gcggtgtccg
      181 gaggaagcgt ggatggtcga tggccgcatt cgccagtgcc ccgctgatgg aacgcgtgcg
      241 ccggacatcg aacgcagcgc catcgtccag gtcataatta tatgtgggaa cgagcagtgt
      301 tccatccggt cctgtcgcat cggcgaacgc atcaagcagg tcagcggcgc ggaaacgctt
      361 gccagaccgt cgtgtgttcc aggccatacg tgtcaggtcg gccatcaaca ggatcgtttc
      421 cccggacctg atgcccaggc gtaccggcca ggtgctgata tcgttcgtgc cggtgttcaa
      481 tgcttgcgca tttgcaacag ttccggcgca tagccgagcg accgccagaa cgcgagcccg
      541 gcctcgttgc gatggaccac ctgcagttca atgctcaaca cgcttctggt gcgcagccat
      601 tcatgcagcg aagcggcgag agcccgtgcg atgcccgaac gccgatgtgc aggggtgacg
      661 tacaggtgcg tgacatgccc gatacgcccg cctcccaagt gttccggcgc gagcttcacg
      721 gcggcgtgcg cgaatccgat gacctcccca tcccattccg ccacggcgat ccgtccgaag
      781 cgctcgagtc ctccgatcac accatcaagc caggtgcggc caccatcggg ggccaggcgt
      841 tggcccatgc cctgttccgc catttccgca tgcatcgttc cgaagagggc cggaaccttg
      901 gcgagagccg ggtcatcggc gcgttgaaca gcccgtacgg tgaagttgct catgcgtcga
      961 cgaacatgga gatgacacct gccaccgtgg tggccttcct gtgccccagt gcgcgctcaa
     1021 gatcgacctg ttttccggta gcaccttcca ggtcggtgat cagatcgacg aagctcaaag
     1081 agtccagaat accgctgcgt accaggtcca ggtcgtccgt gaggtcgtcc ggtgtgaggc
     1141 cgagccg
//
LOCUS       DAOQOC010001181         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_120525_length_1147_cov_2.620879, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001181 DAOQOC010000000
VERSION     DAOQOC010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120525_length_1147_cov_2.620879"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PL106_15370"
     CDS             <1..1026
                     /locus_tag="PL106_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA34508.1"
                     /translation="MSASESERVGFLHMHQGGQYVILAQGHDKLGRSYMANLKRILIF
                     RGLILLLIIFISGWLFAGRFLKPISDIVGQANLITYRNMDQRLRGTGTDDEIGRLIST
                     FNSMLDRLEVSFNAQKKFVSNASHELRNPLAAIKGHVDVALQKDRDNEDYREVLRGVS
                     MDIARMRTLTDHLLELANSDEGLAQHFKEVRIDDLLWNAREEMLRQHPDYTVTVHFGH
                     LMDSDRGITCKGDPDLLGTAFMNLMDNACKFSEDRAVEVRITAENGSIRLLFIDNGIG
                     IAESDLRHVYDPFFRGGNTGDFHGHGIGLSLVQRIIKLHGGRTRIRSKVSEGTTVELT
                     LPNLSGA"
BASE COUNT          252 a          335 c          363 g          197 t
ORIGIN      
        1 atgtcggcct ccgaaagcga gcgcgtcggg ttcctccaca tgcatcaggg agggcaatac
       61 gtgatcctgg cccagggcca tgacaagctg ggcaggagct acatggcgaa cctgaagcgg
      121 atcctgatct tccgtggact gatcctcctt ctgatcatct tcatcagcgg gtggctgttc
      181 gccggtcgtt tcctgaaacc gatctcggac atcgtcggac aggcgaacct gatcacctac
      241 aggaacatgg atcagcgatt gcggggtacc ggtacggacg atgagatcgg ccgactgatc
      301 tcgaccttca acagcatgct cgaccggctg gaggtgtcct tcaacgcgca gaagaagttc
      361 gtatcgaacg cctcgcacga gctgcgcaat ccgctggccg cgatcaaagg ccatgtcgat
      421 gtggcactgc aaaaggaccg tgacaacgag gactaccggg aggtactgag aggagtgtcg
      481 atggacatcg cccggatgcg gacccttacc gaccacttgc ttgaactggc gaacagcgat
      541 gaaggactgg cccagcactt caaggaagtt cgcatcgacg acctgctctg gaacgcacgg
      601 gaggagatgt tgcgccagca tcctgactac acggtgacgg tgcacttcgg tcacctgatg
      661 gacagcgatc gcggcatcac ctgcaagggc gatcccgacc tgctgggcac cgcgttcatg
      721 aacctcatgg acaacgcctg caagttctcg gaggacaggg cggtggaagt gaggatcacc
      781 gcggagaacg ggtccatccg gctcctgttc attgacaacg ggatcgggat cgcggaatcc
      841 gacctcaggc acgtgtacga cccgttcttc cgcggaggca acacgggaga cttccacggc
      901 cacgggatcg gcctttccct ggtacagcgg atcatcaagc tgcacggggg gcgcacccgg
      961 atccggagca aggtgagcga aggcacaacg gtcgagctga cgctgccgaa cctgagcggg
     1021 gcatgaggtg gccgaacacg cgaggggtga ggaaaggcca cgtcatcaag accatcgcga
     1081 ctcttgtgcc ccggatagct tgggcgcatg tccgcccgga acggatactg cccgataagg
     1141 cacctgt
//
LOCUS       DAOQOC010001182         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_120898_length_1145_cov_3.883486, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001182 DAOQOC010000000
VERSION     DAOQOC010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120898_length_1145_cov_3.883486"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PL106_15375"
     CDS             <1..594
                     /locus_tag="PL106_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNA34509.1"
                     /translation="KGKNGFTVRAEDQFGNATEIVFGIRREAPVVVATPPKPDPTKST
                     EKPADKPTVSSATGITWVVLIDNSDYKNMPAPQGQANDRGDIQRSFSKYNVQRTITKR
                     NLTKSQMERLFNIELRDLVRTNKVNTILVGYTGHGRTMGGRTYWVPVDGKKDDIYSCY
                     NYGSLKSLLENYSATVSNTLVVSKAAGTDPSFYELTR"
     gene            646..>1145
                     /locus_tag="PL106_15380"
     CDS             646..>1145
                     /locus_tag="PL106_15380"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA34510.1"
                     /translation="MRILPAILFLSLAAPIFGQQYYFENVSVMQGLPASKVYCVLQDS
                     SGLVWLGTESGLASYDGNSITAFGPHDGMAPGGVRSLFLDAEQRLWCGHLGGGVSIGH
                     GRRFRMLTLADGPIKGDVTSIVRDPQGAMWIGAFGDGARRIKEVPEDAPATAERFSEG
                     KGPGEK"
BASE COUNT          229 a          364 c          363 g          189 t
ORIGIN      
        1 aagggcaaga acggcttcac cgtgcgggcc gaggaccagt tcggcaatgc caccgagatc
       61 gtgttcggca tccgccggga ggctcccgtg gtcgtggcga ccccgccgaa gccggatccc
      121 acgaagtcga ccgaaaaacc ggccgacaaa ccgaccgtga gcagtgccac cggcatcaca
      181 tgggtggtgc tcatcgataa ttccgactac aagaacatgc ccgcgccaca gggccaggcc
      241 aacgatcgtg gcgacatcca gcgttcgttc agcaagtaca acgtgcagcg caccatcacc
      301 aaacggaacc tgaccaagag ccagatggag cgtctcttca acatcgagtt gcgcgacctg
      361 gtgcgcacca acaaggtgaa caccatcctc gtgggctaca cgggccatgg acgcaccatg
      421 ggtggccgca cctactgggt gcccgtggac gggaagaagg acgacatcta cagctgttac
      481 aactacggct cgctgaagag ccttctggag aactacagcg caacggtgag caatacgctc
      541 gtggtgagca aggcggccgg taccgatccc agcttctacg aactgacgcg gtaacagggc
      601 ccgcgctgcg gcattccgtc gcagccgcta ctttgggggc cctccatgcg catcctcccc
      661 gccatcctct tcctgtccct cgccgccccg atcttcgggc agcagtatta tttcgagaac
      721 gtgtccgtga tgcagggcct gcccgcgagc aaggtctatt gcgtgctgca ggacagttcg
      781 ggcctggtgt ggctgggtac cgaatcgggg ctggccagct acgatggcaa ttccatcacg
      841 gccttcggcc cccatgatgg catggccccg gggggcgtgc gcagcctctt tctggacgcg
      901 gagcaacgcc tgtggtgcgg gcatctgggc ggtggggtca gcatcggtca cgggcgcagg
      961 ttccgcatgc tcaccctggc cgatggaccc atcaagggcg atgtcacctc catcgtgcgg
     1021 gatccccaag gcgccatgtg gatcggagcc ttcggcgacg gtgcccgccg gatcaaggag
     1081 gttcctgagg atgcaccggc cacggctgaa cggttcagcg agggcaaggg tcccggagaa
     1141 aagat
//
LOCUS       DAOQOC010001183         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_121163_length_1144_cov_2.977043, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001183 DAOQOC010000000
VERSION     DAOQOC010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121163_length_1144_cov_2.977043"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PL106_15385"
     CDS             complement(<1..1074)
                     /locus_tag="PL106_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34511.1"
                     /translation="MLLDPVTTTPKLGEEATVTGKAMSYAGVPLDGAQVRWKVLREAR
                     MPWWCSGYWRNAIAWGQPTEVANGTATTDASGAFTIRFTAQADQAITREADPVFQYTV
                     ETSVTDINGETQESGTRLSVGYRSIDIDLQVGDAIDRTSTDSLRIEVRNLNGQPVDVP
                     MNITIARLNAPPKPLRDRLWERPDRMTLTREQHSVRFPDDVYAQENDPLTWPKETVVH
                     ERSDWRSRGKSLPIGSITGWDVGMYLIEVTAKDKDDRPVSVRKAVVVYDPEVQHTGFV
                     NDAFHVEAVRSRVEPGGKAVLLLSSALPEARVLMEVERNGTIAVRRWFTLNKGQQRVD
                     LEVLEGVDDLPRVPAEPAVHRPGD"
BASE COUNT          166 a          378 c          366 g          234 t
ORIGIN      
        1 gtcgccgggg cgatgaaccg ctggctccgc tgggacgcgc ggaagatcgt cgacaccctc
       61 gagcacttcg agatccacgc gttgctggcc cttgttcagg gtgaaccagc ggcgaacggc
      121 gatcgttccg ttgcgctcca cctccatcag cacccgggcc tcgggcagcg cgctgctcaa
      181 cagcagcacc gcctttccgc cgggttccac gcgcgaccgc acggcttcga catggaacgc
      241 atcgttcacg aagccggtgt gctgcacctc gggatcgtac acgaccaccg ccttgcgcac
      301 cgagaccggc cggtcgtcct tgtccttggc ggtcacctcg atcaggtaca tgccgacatc
      361 ccatccggtg atggacccga tcgggaggga ctttccacgt gaacgccaat cggaacgttc
      421 atgcaccacg gtctccttgg gccaggtgag cgggtcgttc tcttgggcgt acacgtcgtc
      481 cggaaagcgg acgctgtgct gttcccgggt gagcgtcatc cggtcgggac gctcccagag
      541 gcggtcgcgc aagggcttcg gtggggcgtt gaggcgcgcg atggtgatgt tcatcggcac
      601 atccaccggt tgaccgttca ggttcctcac ttcgatgcgc agactgtccg tgctcgtccg
      661 gtcgatcgcg tcgcctacct gcaggtcgat atcgatactg cggtagccaa cgctcagccg
      721 cgtgccgctc tcctgggttt cgccgttgat gtccgtgacg ctggtctcca ccgtgtactg
      781 gaagaccggg tcggcctccc gggtgatggc ctggtccgcc tgtgcggtga accgtatcgt
      841 gaaggccccg gaggcatcgg ttgtcgctgt gccgttcgcg acctccgtgg gttgtcccca
      901 cgcgatggcg ttgcgccagt atccgctgca ccaccacggc atgcgggctt cgcgcagcac
      961 tttccaacgc acttgggcac catcgagggg aacgccggcg tagctcatgg ccttgccggt
     1021 aacggtggcc tcctccccga gcttcggtgt ggtggtaacc gggtcgagga gcacttcgaa
     1081 ggtcggccgc ttgtactctt caacacgtat gcttctgctg ccgtgcgcct cgctgatggt
     1141 catt
//
LOCUS       DAOQOC010001184         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_121202_length_1144_cov_2.535354, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001184 DAOQOC010000000
VERSION     DAOQOC010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121202_length_1144_cov_2.535354"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PL106_15390"
     CDS             complement(<1..528)
                     /locus_tag="PL106_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34512.1"
                     /translation="MRTLPSIQPWVALLVAGLCAHACRSQVTRHFFRWPLSNGVELNF
                     NSQPNCTAEVVPFLFISHHVAGSDGTTGDLAFIAELELYDRDRTVLPDASPPCGLICG
                     ALPHPGNDSLWYLFGIAGDDTLIYSVFNRQLRNGAGDLTDVRNVPICTFPGSVKVTAL
                     SSDNGAEYRLVIHVNN"
     gene            complement(525..926)
                     /locus_tag="PL106_15395"
     CDS             complement(525..926)
                     /locus_tag="PL106_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34513.1"
                     /translation="MIGSMLANHAHAQPTFSVQNHANHDLHRIYLYCIRPDYSAVTYV
                     YGPIPNDQQTYTYQCPEAEHIKALKLYCSPWDVIVDWECPTYCEFINWPNATDAYPDP
                     CDNGEKYYFRFGISGEPNCIDNGFLGISSNP"
BASE COUNT          242 a          308 c          349 g          245 t
ORIGIN      
        1 attgttcacg tggatcacca agcgatactc ggccccgtta tcggacgaaa gagccgtgac
       61 cttcaccgag ccgggaaagg tgcagatggg cacattccgt acatcggtga ggtcgcccgc
      121 gccgttgcga agctggcggt tgaagaccga atagatcagc gtatcgtccc cggcgatgcc
      181 gaacagatac cagagactgt cgttccccgg atggggaagc gcaccgcaga tcaacccgca
      241 cggtggcgac gcgtcgggga gtacggttcg atcccggtca tagagttcaa gttccgcgat
      301 gaaggccagg tctccggtgg tgccatcact ccccgccaca tggtgggata taaagaggaa
      361 cggcaccact tcggccgtgc agttcggctg gctgttgaag ttcaattcga cgccgttgga
      421 gagcggccag cggaagaaat gccgtgttac ttgcgagcga cacgcatggg cacagagacc
      481 tgcgaccagg agtgcgaccc acggctgtat ggagggtaac gttctcatgg gttggaactg
      541 atgccaagaa agccgttatc gatgcaattg ggctcgcctg atatcccgaa gcggaagtag
      601 tacttctctc cattgtcgca tggatccgga taggcatccg ttgcgttagg ccaattaatg
      661 aattcgcagt aggtcgggca ctcccagtcc acgatcacgt cccatgggct gcaatagagc
      721 ttcagggcct tgatgtgctc ggcttcgggg cattggtacg tataggtctg ctggtcattc
      781 gggatcggcc cgtacacgta cgtcacagcg ctatagtccg gtcggatgca atacaggtag
      841 atccggtgca gatcgtggtt cgcgtggttc tgaacgctga acgtcggctg ggcatgcgca
      901 tggttcgcca acatcgaacc gatcatgatg cacatggcga caagggcgcc gtgcagggca
      961 tcatggaaga gccttgtccg cagagccttg tccgcagagc cttgtccgaa ggtttttcat
     1021 gagatcagga gttttcggtg aactgtaaat gtacacgttt gtttccaacg tgcatacctg
     1081 gtgtaaaact cttgtccact gggccgcgcc tgccagccag cgatgcccgc tattcccctt
     1141 gcat
//
LOCUS       DAOQOC010001185         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_122070_length_1140_cov_2.990783, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001185 DAOQOC010000000
VERSION     DAOQOC010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122070_length_1140_cov_2.990783"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="pgk"
                     /locus_tag="PL106_15400"
     CDS             complement(<1..701)
                     /gene="pgk"
                     /locus_tag="PL106_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNA34514.1"
                     /translation="MLTMDSYNFKGKKALVRVDLNVPQDANGKVTDDTRARAIVRTVK
                     KITNDGGSAILMSHLGRPKGKVNPAMSLRPIAEHLSGLLGQPVLFATDCVGPDAKAKA
                     AALKPGEVLVLENLRFHPEEEAGDEAFSKALGELGDVYVNDAFGTAHRAHASTTIIAK
                     FFPKDKLFGHLMQAEIDSVGKVLGAPQRPMTAIVGGSKVSSKIDVLKNLIGKCDELII
                     GGGMANTFVKAQGGQT"
     gene            814..>1140
                     /locus_tag="PL106_15405"
     CDS             814..>1140
                     /locus_tag="PL106_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNA34515.1"
                     /translation="MQFAHIVGHAALKATLIGNIREGRVPHAQFFLGPRGSGTLPLAL
                     AYARYLLCAEKDTVDSCGRCPSCQMVAKLAHPDLHLTFPIFLSEKIKHCDTFIAEWRE
                     AVLMEPY"
BASE COUNT          179 a          347 c          367 g          247 t
ORIGIN      
        1 gtttgccctc cttgggcttt aacgaaggtg ttggccattc caccgccgat gatgagttca
       61 tcgcatttgc cgatcaggtt cttcagcacg tcgatcttgc tgctcacctt gctgccgccc
      121 acgatggcgg tcatggggcg ctgaggtgcg cccagcacct tgcccacgct gtcgatctcg
      181 gcctgcatca ggtggccgaa gagcttgtcc ttggggaaga acttcgcgat gatggtggtg
      241 ctggcgtgtg cgcggtgcgc ggtgccgaag gcatcgttca cgtacacatc gcccagctcg
      301 cccagtgcct tgctgaaggc ctcatcgccc gcttcttctt cggggtggaa gcgcaggttc
      361 tccagcacga gcacttcgcc gggtttcaag gcagcggctt tcgctttcgc gtccggcccc
      421 acgcagtcgg tggcgaagag cacgggttgg ccgagcagtc cactgaggtg ctccgctatc
      481 ggacgcaggc tcatggccgg gttcaccttg cccttcgggc ggccgaggtg gctcatgagg
      541 atggcactgc caccgtcgtt ggtgatcttt ttcaccgtgc gaacgatggc gcgggcgcgg
      601 gtatcgtccg tcaccttgcc gttcgcgtcc tgcggcacat tgaggtccac gcgcaccagc
      661 gcttttttgc ccttgaagtt gtaagagtcc atggtcagca tgcggcgaaa gtaggatcac
      721 ttttcgggcc aaagccacag gtcgcaagcc tccagcttgt ggcctgtggc ctgaaacgcg
      781 cgggggcatt gtgccggaga gtacacttgc agcgtgcaat tcgcccacat cgtcggccat
      841 gccgcactga aggccacgct catcggcaac atccgtgagg gccgggtgcc gcatgcgcag
      901 ttcttcctgg ggccgcgggg cagtggaaca ctcccgctcg cgttggccta tgcgcgttac
      961 ctcttgtgtg cggagaagga cacggttgac agttgcggcc gatgcccgag ttgccagatg
     1021 gtggccaagc tggcgcatcc ggacctccat ctcacgttcc cgatcttcct gagtgagaag
     1081 atcaagcact gcgacacgtt cattgcggag tggcgggagg ccgtgctgat ggagccgtac
//
LOCUS       DAOQOC010001186         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_122706_length_1137_cov_3.533272, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001186 DAOQOC010000000
VERSION     DAOQOC010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122706_length_1137_cov_3.533272"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..>1137)
                     /locus_tag="PL106_15410"
     CDS             complement(418..>1137)
                     /locus_tag="PL106_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34516.1"
                     /translation="IAIEKTGEESAILQIIGDEALYGPDAIVEPYAEEQGMAPSKGPS
                     APELHGFFVTVNCWFWEPVPWFFSDRWYAYHSPWYWGYYPPWWNTWAPYDWFIWHGWW
                     GYHHHWYRPWGSCRVGSAQALYLPRRAQSAIVRERYRDVRARYEKNPARLQKGPADGL
                     QKERHGKDAVRERPVTPDRPQRAKAPEARPSKEPGKNGVARPGKEPRQPEARPARRPA
                     KARPAPAPRPTPRPSPRPRGR"
BASE COUNT          203 a          353 c          366 g          215 t
ORIGIN      
        1 tggcgctcct cacttcgccg gagcggctac gcataggcgc agtcggcggg cagcgtgaca
       61 gtggggacat gagtgtccaa gagcactgtt cttgaacact cccgatgagc tgtcacgctg
      121 agcctgccga agcgctgcaa caagccggta tcgtttctga atacgcgatc ctcagcacgt
      181 tcaggccggt ctgaacacta ttttgctcgg aaatggggga ttgggcgagt cacccgatca
      241 aatacggatg tgcccggtca tcaaggcttg cagcgtacga tcgggttgga actcgcggcg
      301 atcactgaat gcaggaagga tgggacgctt tcctgaagaa ggggtagtac ggaaagcgga
      361 aggcccccgg tgatcacgac caccgggagc ctcctgttat ccccgtatgt ggaggaccta
      421 tctgccgcgt ggccgtggcg aaggacgtgg ggtcggccgc ggcgcgggtg cgggtcgcgc
      481 tttagctggc ctgcgcgcgg gcctggcctc cggctggcgt ggttccttgc cgggccgggc
      541 cactccgttc ttaccgggtt ccttgctggg gcgggcttcc ggagccttcg cccgttgagg
      601 gcgatccggg gtcaccggcc gctcgcgtac cgcatctttc ccatgccgct ctttctgcaa
      661 gccgtcagcc ggtcccttct gcaggcgggc ggggttcttc tcatagcgcg cacgcacatc
      721 ccggtaccgc tcccgaacga tcgcactctg cgcccgacgg ggcaggtaca acgcttgtgc
      781 ggagcccaca cgacaactac cccagggccg gtaccagtgg tgatggtagc cccaccagcc
      841 gtgccagatg aaccagtcat acggggccca tgtgttccac cacggcggat agtagcccca
      901 ataccaaggt gagtgatagg cgtaccagcg gtcgctgaag aaccagggca ccggctccca
      961 gaaccagcag ttcaccgtga cgaagaagcc atgcaattca ggagccgaag gacctttgga
     1021 aggggccatg ccctgttcct cggcgtatgg ctccacgatc gcatccggtc cgtacagggc
     1081 ctcatcgccg atgatctgga ggatggcact ctcctcgccg gtcttttcaa tggcgat
//
LOCUS       DAOQOC010001187         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_122925_length_1136_cov_3.939870, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001187 DAOQOC010000000
VERSION     DAOQOC010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122925_length_1136_cov_3.939870"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PL106_15415"
     CDS             complement(<1..484)
                     /locus_tag="PL106_15415"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNA34517.1"
                     /translation="MRYPPLSAATYCEHRARYRQAMEKGGLAIFHSNDIMPTSADGTM
                     PFKQASDIFYLSGIDQEETVLLLFPGALDPKDREILFVRETSELIAIWEGAKFTQQEA
                     SALSGIATVLWTGSYEATIKRLVPQCDHLYLNSNEHLRQGNEVETREERKNKELRAQY
                     P"
     gene            693..>1136
                     /locus_tag="PL106_15420"
     CDS             693..>1136
                     /locus_tag="PL106_15420"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNA34518.1"
                     /translation="MSIPSYTIAQLPPAQAVETPAVLRKAAEAHRYLAELKGVAGTIP
                     NERILVDTLSLQEAKDSSAIENIITTDDEVYRSDYLARTFTTPAAKEVHRYAEALKLG
                     FEEVRRSGGIGLNTILRVQAKLEDNTAGLRKLPGTVLKNERTQEVV"
BASE COUNT          219 a          314 c          375 g          228 t
ORIGIN      
        1 aaggatactg cgcccgcagc tccttgttct tgcgctcctc gcgcgtctcc acctcgttgc
       61 cctggcgcag gtgctcgttg ctgttcaggt acaggtgatc gcactgcggc accaagcgct
      121 tgatggtcgc ttcgtagctg cccgtccaga gcaccgtggc gatgccgctc agcgcgctcg
      181 cttcctgctg cgtgaacttg gcgccctccc agatcgcgat cagttcgctg gtctcgcgca
      241 cgaagaggat ctcgcggtcc ttcgggtcca acgcaccggg gaagagcagc aggaccgtct
      301 cctcctggtc gatgccgctg aggtaaaaga tgtcgctggc ctgcttgaac ggcatggtgc
      361 catcggcgct ggtgggcatg atgtcgttgc tgtggaagat ggcgaggccg cctttctcca
      421 tggcttggcg gtagcgggcg cggtgttcgc agtaggtggc ggcggaaagg ggggggtagc
      481 gcatggtgat ccgccgaagc ctcggcgtag gcgattgggg aaagatgaag tgcgaaagta
      541 ggagggtgga aggcggcgaa agctgcttgg ctttacgagc gctgaccggc ctcggccggg
      601 tcgtttgtgg cttttcaaac gaactataca tattctaaca atatgtagag gaagacgcga
      661 cattctttac atatttgcat ggacctgtat agatgagcat cccttcctat accatagctc
      721 aactacctcc ggcccaggcg gtggagaccc cggccgtgct gcggaaggcc gccgaggcac
      781 accgttatct ggctgagttg aagggagttg ctggtaccat accgaacgag cggatcctgg
      841 tggataccct ctccctgcaa gaggccaagg acagttcggc catcgagaac atcatcacca
      901 ccgacgacga ggtgtaccga agcgattacc tcgctaggac ctttaccaca cctgcggcca
      961 aggaggtgca ccgttatgct gaggccttga agctcggctt cgaagaggtg cgcaggtcgg
     1021 gcggaatcgg actgaacacg atcctgcggg tgcaggcgaa gttggaggat aacacggccg
     1081 gtcttcggaa gctgccgggc acggtgctga agaacgagcg cacccaggaa gtggtg
//
LOCUS       DAOQOC010001188         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_122955_length_1136_cov_3.152636, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001188 DAOQOC010000000
VERSION     DAOQOC010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122955_length_1136_cov_3.152636"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..878
                     /locus_tag="PL106_15425"
     CDS             228..878
                     /locus_tag="PL106_15425"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNA34519.1"
                     /translation="MMLARLLSWFWPVRMRTEEGTLGTVELTWEQGRLVVNSANANQS
                     FGSLHRVWQEAFAKAGVATERPLTTLLLGYGGGSAVHILQKELGLETAITGVDMDPLM
                     LRIAREFFPPPHPERVRMVQADAFTYVREAKETFDLVVVDLFRDLDFTEGVESPEFLR
                     DLKHILTPGGTLLLNTIAHDERSTARSARSGRELRLLFNHVEEHPCQEHNRVFIAW"
BASE COUNT          228 a          320 c          361 g          227 t
ORIGIN      
        1 tgaagagcat gagcaccaag gccgcacccg tggtgaccaa accgtacttc agcagcttgg
       61 ccagcttgta ccgggtgatg gccgagaaat agagcatggt gcaggtggca ggagacgatc
      121 gcaggatgct tacagacgcc tgcgccccct cgcaatggac acgcacgctc cgccttctgc
      181 tgtgatcaaa atggccgcga aactacggtc atggcatctt tgggcccatg atgctggcgc
      241 ggttgctcag ttggttctgg ccggtgcgga tgcgtacgga ggaaggaaca ttgggcacgg
      301 tggagctcac atgggaacag ggtcgcttgg tcgtgaacag tgccaacgcc aaccagagtt
      361 tcggtagctt gcacagggta tggcaggagg ccttcgccaa agccggtgtt gccacagaac
      421 gcccgctcac cacgctgcta ctgggctacg gagggggaag tgcggtgcat atcctccaga
      481 aggaactcgg actggagacg gcgatcaccg gagtggatat ggatccgttg atgttgagga
      541 tcgcgcggga gttctttcct ccgccccatc ctgaacgggt gcgcatggtg caagccgatg
      601 cattcacgta cgtaagggag gccaaggaaa cttttgacct ggtggtggtg gacctgttcc
      661 gtgacctgga cttcaccgaa ggcgtcgaaa gcccggaatt cctccgcgac ctgaagcata
      721 tcttgacgcc tggtggcacg ctcttgctga acacgatcgc tcatgacgaa cggagtaccg
      781 cacgcagcgc acgttcgggc cgggaactac gtctattgtt caaccatgtg gaagagcacc
      841 cctgtcagga gcacaatcgg gtgttcattg cttggtgaat cgactggcag gcagccgctt
      901 gcgtgcgagg gtgccgtggt ccgaaatttg tcgaacgccg ttttccggaa cgcattgacc
      961 agaacgaggc cccttccctt gcttgtcgcc ctgctgggtg cggtgttcgc cttgtcagga
     1021 tcggcgcaac ccgtctgttc catcaacttg ggcgccgatg tgaccatttg tgcaggggag
     1081 tccaccacca tcaccgcgcc ggccggctac ccgaactacc tgtggagcac aggggc
//
LOCUS       DAOQOC010001189         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_123217_length_1135_cov_2.660185, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001189 DAOQOC010000000
VERSION     DAOQOC010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123217_length_1135_cov_2.660185"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /locus_tag="PL106_15430"
     CDS             complement(<1..>1135)
                     /locus_tag="PL106_15430"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNA34520.1"
                     /translation="IAREQELLSSMKVDADDRASATNLDKHEHELAAFIEDLRKDHPG
                     YFALRYQEPVIGLAELRAHLVTPQRDLLLYAFAGEHLYMLAVRSDTALLVRTSRKDIA
                     EKTRALGTSIRQNDQGSFVRLSRALYDQVFRPVADLLHNEELLIVPDGDLQALNFEVL
                     LFEDPGSDRPDRHMLIRKHAIAYLLSATTSVQFADLHRTRSNKVLAFAPGFSDEMKQA
                     YIEGYGDRSDVDRNFLGYVRQPFALMAAEELGRTLSARVMTGTDASEKHFRGLATGYG
                     ILHMGTHAELNSNSPMYSRFVLGKDGAGEDAVGDGYLHAYEIYELDLSAQLAVLTACE
                     TGIGTAEAGEGARSLGYSFAYAGCPSLITSLWSIDEKTSSDIIT"
BASE COUNT          198 a          326 c          389 g          222 t
ORIGIN      
        1 gggtgatgat gtcggaactc gttttttcgt cgatgctcca caacgaggtg atcaggctgg
       61 gacaaccggc gtacgcgaaa ctgtagccca gcgatcgcgc accctcgccc gcttcggcgg
      121 tgccgatacc ggtttcgcag gcggtgagca cggcgagttg tgcggacagg tccagctcgt
      181 agatctcata tgcgtggagg taaccgtccc ccacggcatc ctcgcccgcg ccatccttgc
      241 cgaggacgaa gcgggagtac atcggcgagt tgctgttcaa ttcggcgtgg gtacccatgt
      301 ggagaatgcc gtaaccggtc gccaggccgc ggaaatgctt ctcgctggca tcggtgccgg
      361 tcatcacgcg ggctgaaagc gtgcggccga gttcttccgc ggccatcagg gcgaagggtt
      421 gtcgcacata accgaggaag ttccggtcca cgtcggaccg atcaccgtag ccttcgatat
      481 aagcctgttt catctcatca ctgaaccccg gggcgaacgc gaggaccttg ttcgaccgtg
      541 tccggtggag gtccgcgaat tgtacggagg tggtggcact caggaggtag gcgatggcgt
      601 gcttccggat gagcatgtgg cgatcgggcc ggtcggatcc cggatcctcg aacaggagta
      661 cctcgaagtt gagggcttgc aggtcgccat cgggcacgat caggagttcc tcgttgtgca
      721 gcaggtccgc aacgggccgg aatacctgat cgtacagggc gcgggacagc cggacgaagg
      781 atccctggtc gttctgccgg atggaggtgc ccagcgcccg ggtcttctcg gcgatgtcct
      841 tccgggatgt gcgaacgagc agggcggtat cgctgcgcac cgccagcatg tacaggtgtt
      901 cgcccgcgaa agcgtaaagc agaaggtcgc gttgcggcgt gaccaggtgt gcgcgcagct
      961 cggccagacc gatgaccggt tcctggtagc gcagggcgaa atagcccgga tggtccttcc
     1021 gcaggtcctc gatgaacgcc gcgagttcgt gttcgtgctt gtcgaggttc gtggcggatg
     1081 cgcggtcatc ggcatccacc ttcatgctgg agagcagttc ctgttcgcgc gcgat
//
LOCUS       DAOQOC010001190         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_123229_length_1135_cov_2.535185, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001190 DAOQOC010000000
VERSION     DAOQOC010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123229_length_1135_cov_2.535185"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1135
                     /locus_tag="PL106_15435"
     CDS             <1..>1135
                     /locus_tag="PL106_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34521.1"
                     /translation="IMCATGLFLVTVEQRKGYLIVLGIAAACYLPNLPLFFAQLGWKG
                     LDEWLAPPGPDWILKYAWWIGHCSMLLCGALGLLLVLSGAMRIKHRSISRPVWAIVLL
                     WGLLPLIIGYAYSVLRAPVLQYSVVLFSFPYLVIGALAGLRHVKHTYALGIAGALAIF
                     SVLTLVTDRKHYQLFYQSKYEEIVRGTVEAERSGSLAIVHIPTEVLNFYRDRWNIGEN
                     AAPAVNLHLRPASTLDSALGSTTAKTVFYGQTSQAQPENIAKVQMRFPFMVERHDRVE
                     GQLFRFAARPVEQPIDDMQGTSTRTPEALEGKGWEVDAKIPVMTDTTASAYGGVRPKR
                     WDFTGHEYGAVYDGPVYELAKGPNDVIEVRADLHSLDPACDMSL"
BASE COUNT          219 a          343 c          350 g          223 t
ORIGIN      
        1 gatcatgtgc gccacgggac tgttcctggt aacggtggag caacgcaaag gctacctgat
       61 cgtactgggc atcgcggccg catgctacct gccgaacctg ccgctcttct tcgcgcaact
      121 cggctggaaa ggtctggacg agtggctcgc acctccaggg ccggattgga tcctgaagta
      181 cgcctggtgg atcggccatt gttccatgct gctctgcgga gcgctcggcc tgttgctggt
      241 cctctcaggt gccatgcgga tcaagcaccg ctccatctcg cgcccggtat gggcgatcgt
      301 tctgttgtgg ggtctgctgc cgttgatcat cggctacgcg tattccgtgt tgcgagcacc
      361 cgtgctccaa tacagcgtgg tgctcttctc attcccctat ctggtcatcg gggcactggc
      421 cgggttgagg cacgtgaaac acacctacgc cctgggtatc gcaggagcac tggccatttt
      481 ctccgttctc accttggtta cggaccgcaa gcactaccag ttgttctacc agagcaagta
      541 cgaggagatc gtacgcggga ccgtggaagc ggaacggtcc ggttcgctgg ccatcgtgca
      601 tatccccacg gaggtattga acttctaccg tgatcgttgg aacatcggag agaatgcggc
      661 ccctgcggtg aacctgcacc tccgtccagc ctcgacattg gacagcgcat tggggagcac
      721 cacagcgaaa accgtgttct acggacagac ctcccaggcc cagccggaga acatcgccaa
      781 ggtccagatg cgtttcccct tcatggtgga gcgccatgac cgggtggaag gacaattgtt
      841 ccgcttcgcg gcacgtcccg tggaacaacc gatcgacgac atgcaaggga cttccacccg
      901 gacacccgag gcactggaag gcaagggctg ggaggtggat gcgaagatcc ccgtgatgac
      961 ggataccacg gcaagcgctt acggcggtgt gcgacccaag cgctgggact tcacgggaca
     1021 tgaatacgga gcggtgtacg acgggccggt ctatgaactg gccaaaggcc cgaacgacgt
     1081 gatcgaggtg cgcgcggacc tccattccct ggacccagcg tgcgacatga gcctg
//
LOCUS       DAOQOC010001191         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_123379_length_1134_cov_3.828545, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001191 DAOQOC010000000
VERSION     DAOQOC010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123379_length_1134_cov_3.828545"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PL106_15440"
     CDS             complement(<1..374)
                     /locus_tag="PL106_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNA34522.1"
                     /translation="MNKPLDPETNVLRTLLVDAPPEVIWKALTDPDITRLYTSTDISL
                     GLKAGQPVQWFSREEDKTRKLVAKGNVLALVPNVRLRYTNYVVSGGLPDEPGSHTTVD
                     LHLVPEADGRTRVELWEGDFAGL"
     gene            complement(520..>1134)
                     /locus_tag="PL106_15445"
     CDS             complement(520..>1134)
                     /locus_tag="PL106_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNA34523.1"
                     /translation="PVDLARRVMDGSGHVLISGMGDFEFAHREKVLLEDDEYFFDQHR
                     FEQWQEVAGTQVVKLDHSGGGRGKFGTVGAVALDQQGHLAAATSTGGMTNKRWQRIGD
                     SPIIGAGTYANDSTCAVSCTGNGESFIRAVAAHDVSALMEYKGLALAEAVREVVHRKL
                     PLLDGEGGLIAIDRNGHIVLDFNCEGMYRGHCGADGAVVTAIFR"
BASE COUNT          196 a          371 c          347 g          220 t
ORIGIN      
        1 agacctgcga agtcgccttc ccaaagttcc acccgggtac gaccatccgc ttcgggtaca
       61 aggtggagat ccaccgtggt gtggcttccg ggctcgtcgg gcaagcctcc gctcaccaca
      121 tagttggtgt accgcaagcg cacattggga accaaggcaa gcacattgcc cttggccacc
      181 agctttcggg tcttgtcctc ctcgcggctg aaccattgga cgggttgtcc ggccttgagg
      241 cccaaggaga tgtccgtgct ggtatagagg cgggtgatat cgggatcggt cagtgctttc
      301 cagatgacct caggcggcgc gtccaccagc agggtgcgca gcacattggt ctcggggtcc
      361 agtggtttgt tcatagcgtg ggtggcaggt caggatccgt gccgctttat agacgctgcc
      421 ggaatgacgg agcgtggccc gcccgtgtta aagggagtga atggcccgag gaacggcagg
      481 tcccgcacga cgaacggcgc gttctgcggg cccgggcggt caacggaaga tcgccgtgac
      541 gaccgcacca tcggccccgc aatgcccgcg atacatgcct tcgcaattga aatccaggac
      601 aatgtgaccg ttgcggtcga tggcgatcag cccaccctcc ccgtcgagca acggcagttt
      661 gcgatgcacc acctcgcgca ccgcctccgc cagtgcaagg cccttgtatt ccatgagagc
      721 gctcacatcg tgcgcggcca cggcccggat gaagctttcg ccgttgcctg tgcaactgac
      781 cgcgcatgtg ctgtcgttgg cgtaggtacc ggcaccgatg atggggctgt cgccgatgcg
      841 ctgccagcgt ttgttcgtca tgccgcccgt gctggtggcc gccgcgagat ggccctgctg
      901 gtccagcgcc accgcaccca ccgtgccgaa cttgcccctg ccaccaccac tgtggtccag
      961 cttcacgacc tgcgtaccgg ccacttcctg ccactgctcg aagcggtgct ggtcgaaaaa
     1021 atactcatcg tcctcaagta gcactttctc gcgatgggcg aattcgaagt cccccatgcc
     1081 gctgatgagc acgtgcccgc tgccgtccat cacccttctg gcgaggtcca cggg
//
LOCUS       DAOQOC010001192         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_123777_length_1133_cov_1.860853, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001192 DAOQOC010000000
VERSION     DAOQOC010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123777_length_1133_cov_1.860853"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1133)
                     /locus_tag="PL106_15450"
     CDS             complement(<1..>1133)
                     /locus_tag="PL106_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA34524.1"
                     /translation="IAVTNVTGGDGDYDYAWTFEGAPYGTTPTVNVPAANPTVWYVAT
                     VTDGCGSTATDSVEVGTVPLEPIVIESPDRTVICLGDTTTLEVLSVTGGNGVYTYTWT
                     NAQQQTISQTTSVDVPVPSDAVYTITVEDQCGYSGSEQVNTLIPHPAPFILRLNNDTV
                     LCAGDSLTLWAQVSGGSGYYTVQWEGLDHSDPLLAVSPPVNANYRVYIFDQCGEVITD
                     DVNVGVEQAVADIRETNQGQDDWLFEPATWPTFCRSYRWDLGDGTTSRDRIVTHSYLD
                     LEEHWVKLEVVSFTGCTAVDSTLIRPPGQLFFPNAFTPDGDGINETFGPATRYIESFE
                     MSIFDRWGHLVYHSEDINKPWDGKTGNGKIVQGVYVYRYKAAG"
BASE COUNT          202 a          349 c          346 g          236 t
ORIGIN      
        1 ggcctgccgc tttgtaacgg taaacataaa caccctgaac gatcttgccg tttccggtct
       61 tcccgtccca tggtttgttg atgtcttcgg agtggtacac caagtggccc cagcggtcga
      121 agatgctcat ttcgaacgac tcgatatagc gcgtggccgg accgaatgtc tcgttgatgc
      181 catccccatc gggggtgaag gcgttgggga agaagagttg gcccggagga cggatcaagg
      241 tgctgtcaac ggccgtgcat ccggtaaagc tcaccacttc aagcttcacc caatgttcct
      301 ccaggtccag gtagctgtgc gtcacgatcc ggtcacggct ggtggtgcca tcgcccagat
      361 cccaccgata gctgcggcag aaggtgggcc atgtggccgg ttcgaacagc cagtcgtcct
      421 ggccctggtt cgtttcgcgg atgtcggcca cggcctgttc cacgccgaca ttcacgtcgt
      481 cggtgatcac ttcgccgcac tggtcgaaga tgtacacccg gtagttcgcg ttcacgggcg
      541 ggctcaccgc gagaagcggg tcgctgtggt ccaggccctc ccactgcacg gtgtagtatc
      601 ccgagccacc gctcacctgc gcccacaagg tcaggctatc gcccgcgcac agcacggtat
      661 cgttgttcaa ccggaggatg aagggggccg ggtgcgggat cagcgtgttg acctgttcac
      721 taccggaata tccgcattgg tcctccaccg tgatggtgta cacggcatcc gagggaacag
      781 gcacgtccac actggtggtc tggctgatgg tctgttgctg ggcattggtc catgtgtagg
      841 tgtacacccc attgccgccg gtcacgctca atacttccag ggtggtggta tcgcccaggc
      901 agatcacggt acgatcaggc gactcgatca cgatcggctc cagtggcacg gtcccgacct
      961 ccaccgaatc cgtggccgtg gagccgcacc catcggtcac cgtggccacg taccacacgg
     1021 tggggttggc tgccggcaca ttcacggtag gcgtggtacc gtacggcgct ccttcgaacg
     1081 tccatgcgta gtcgtagtcg ccatcgccac cggtcacatt ggtcacggcg ata
//
LOCUS       DAOQOC010001193         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_124085_length_1131_cov_3.552045, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001193 DAOQOC010000000
VERSION     DAOQOC010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124085_length_1131_cov_3.552045"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PL106_15455"
     CDS             complement(<1..861)
                     /locus_tag="PL106_15455"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNA34525.1"
                     /translation="MRKHLLRHPTAVIALVCSTGVHAHEGMWLPTLLQAIEGNMRTEG
                     MMISAEDIYSLNNGSIKDAVVLFGGGCTAEVVSTQGLILTNHHCGFGSIHRHSTLEHD
                     YLKDGFWAATLADELPNPGLSATFIVRMEDVTDRVLAGVKPGMTESERTAAIDLATAE
                     VAVEAKSEDGYSAVVKPFNYGNNWYLIVSETYRDIRLVGAPPGSIGNFGGDTDNWMWP
                     RHTGDFSVFRIYAGKDNRPAAYAADNVPFKPRRSLTISLDGVKEGDFAMIFGFPGSTQ
                     RYLASPAVAQV"
BASE COUNT          208 a          356 c          339 g          228 t
ORIGIN      
        1 cacctgggcc accgccggac tggccaggta gcgttgggta ctgccaggga atccgaagat
       61 catggcgaag tccccctctt tcaccccatc caaactgatc gtgaggctcc gccggggttt
      121 gaagggcacg ttgtcggcgg cgtaggcggc ggggcggttg tccttgcccg cgtaaatgcg
      181 gaacacgctg aagtccccgg tatggcgcgg ccacatccag ttgtcggtat ccccaccgaa
      241 gttgccgatg gagccggggg gcgcacccac cagtcgtata tctcggtatg tttcactcac
      301 gatcaagtac caattgttgc cgtagttgaa cggcttgacc accgcgctgt acccatcttc
      361 acttttggcc tccacggcca cttccgccgt tgccaggtcg atcgccgccg tacgttcgga
      421 ttcagtcatg ccgggtttca ccccggccag cacacggtcc gtcacatcct ccatgcgcac
      481 gatgaaggtg gccgatagcc cggggttcgg cagttcatca gccagggttg ccgcccagaa
      541 cccgtccttc aggtagtcgt gctccagcgt gctgtgccga tggatcgaac cgaatccaca
      601 atggtggttg gtgaggatca gaccctgcgt actcaccact tcggccgtgc aaccaccgcc
      661 aaagagcacc accgcgtcct tgatgctccc attgttcagg ctgtagatgt cttcggcgct
      721 gatcatcatg ccctcggtac gcatgttccc ttcgatggcc tgcaacaagg tgggtagcca
      781 catgccttcg tgggcatgca cgccagtgga acagacaagg gcaatgacag cggtagggtg
      841 gcgtagcagg tgcttcctca tggggcttgt tcgtcggaca aagatgggcg cgtcgcccca
      901 atagccggaa ccaggcgttg ggacaccccg acctgcgagc ggctgttcgg gctcatccgg
      961 accggatcct tcgggtcctt gagcggtact ggccgggtga tcctaacgcg acctgcggac
     1021 atctcagaaa tcccccgtca tcagccatgc gagcaggacg gtgatcagca cgacgaggaa
     1081 cctattcggg ttcggataca ggggcctctt gtgtgccgat cgcaccacac g
//
LOCUS       DAOQOC010001194         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_124126_length_1131_cov_2.993494, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001194 DAOQOC010000000
VERSION     DAOQOC010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124126_length_1131_cov_2.993494"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PL106_15460"
     CDS             <1..337
                     /locus_tag="PL106_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34526.1"
                     /translation="IDTLRKGQYAFAKGNVIIGMEETDSRPLDLGIVPDPADDHIRVR
                     GRYEGAATLFFDVLDMDGGLVQRTTVAVANTFDRNVPVATLPAGSYLLRAHDGKGGLI
                     GTGRFSVMH"
     gene            617..>1131
                     /locus_tag="PL106_15465"
     CDS             617..>1131
                     /locus_tag="PL106_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34527.1"
                     /translation="MKKTHTPIYRRWSTVISAAALSTIAFGDPAELPAHSMYDMAPPV
                     QLISFTVTQDQQFVRLDWVTASEWNNVKFVLERSTDGEAFGTIGIVLGAGNSSQMITY
                     KWLDRTPPDGVVQYRLQQIDGDGQSTYSPVATIDMGTGPDPVVSPNPIAADQFTLNNV
                     AIGSQLEIRTM"
BASE COUNT          229 a          358 c          336 g          208 t
ORIGIN      
        1 gatcgatacc ctgcggaaag gccagtacgc gttcgcgaaa ggcaacgtca tcatcggtat
       61 ggaggaaacc gacagcagac ccctggatct cgggatcgtt ccagatccgg ccgatgatca
      121 catccgtgtt cgtggccggt acgagggggc ggcaacgctc ttcttcgatg tcctcgacat
      181 ggatggcggc ctggtgcaac gcaccaccgt ggcggtggcg aacacgttcg accgcaacgt
      241 tcccgtggcc acacttccgg cgggcagcta cctgttgcgc gctcatgatg gcaagggtgg
      301 cctgatcggt accggccgct tcagcgtgat gcactgagat cccgggcgat cgacaggtat
      361 ccgatcaccc agtatgggta tgaccatacg ggtcgaccgg tgatgaggcg acagggaaga
      421 acggtcgatc aaccgggcat ggccaggtac aggagcggac ggatgtgaag gtgtccaaag
      481 atccctgcat ctctctggcc tcgttgaact cccctgcccg ggtgcggcta tcgctcgccg
      541 accgggcaag ggacgtcctg cccttcctgt cttggagggt aaccttggtt gcccgcgatc
      601 cgtaggagcc gaagacatga agaaaacgca tacccccatc taccggcgtt ggagcaccgt
      661 gatcagcgcg gccgctctct ccaccatcgc gttcggcgat cccgctgaac tccctgcaca
      721 ctccatgtac gatatggcgc cgccggtcca gttgatcagc ttcaccgtca cccaggatca
      781 acagttcgtc cgcttggatt gggtgaccgc ctccgaatgg aacaacgtca aattcgtact
      841 ggagcgctcc accgatggtg aggccttcgg aacgatcggc atcgtgctgg gtgcggggaa
      901 cagcagccag atgatcacct acaagtggct cgatcgcaca cctcccgatg gcgtagtgca
      961 ataccgcctc caacagatcg atggcgatgg ccagtccacc tactcgcccg tggccacgat
     1021 cgacatgggc acggggccgg atcccgtggt ctcccccaat cccatcgccg ctgaccagtt
     1081 caccttgaac aacgtggcca ttggcagcca actggagatc cgcaccatga a
//
LOCUS       DAOQOC010001195         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_124635_length_1129_cov_2.237430, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001195 DAOQOC010000000
VERSION     DAOQOC010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124635_length_1129_cov_2.237430"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PL106_15470"
     CDS             complement(<1..349)
                     /locus_tag="PL106_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34528.1"
                     /translation="MKKTVLFAAAIFTVSAATAQTGEITSAKGENWLSQDGDWGLTFD
                     ATPLLNYAGNLFNGTAGNGGVALNNIFASNNGSNVIIAGKKLMDANTAYRGRVRIGFG
                     SLKGTELVPTADPN"
     gene            complement(566..>1129)
                     /locus_tag="PL106_15475"
     CDS             complement(566..>1129)
                     /locus_tag="PL106_15475"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNA34529.1"
                     /translation="RAMGAVPRHLFIDDSAFLSLAYADQAFPIGCGQTISQPTTVAVQ
                     SQLLRVEPGMKVLEIGTGSGYQTAVLCELGAKVFSIERHKPLFETTKQRLVRLGYKAT
                     LMHGDGYKGAPLHGPFDRIIVTCGAPEVPEALLGQLKLGGILVIPVGPDGRQKMWSWS
                     KIGADVFDRQEHGSFSFVPMLAHKVRG"
BASE COUNT          229 a          325 c          325 g          250 t
ORIGIN      
        1 cgttgggatc agcggtagga acgagttccg tacccttcag gctgccgaag ccgatgcgca
       61 cacgaccgcg gtaggcggtg ttggcgtcca tgagcttctt gccggcgatg atcacattgg
      121 acccgttgtt gctggcgaag atgttgttca gggccacacc gccgttgccc gcggtgccgt
      181 tgaacaggtt gccagcgtag ttgagcagag gggtggcgtc gaatgtgagg ccccaatcgc
      241 catcctggga aagccagttt tcacctttcg cgctggtgat ctcacccgtt tgggcggtcg
      301 cagccgatac ggtgaagatc gctgcggcga acagtacagt cttcttcatg tttaacgtgt
      361 tggtttgacg tgtttttgcg tgtttagcgt ttgctcgaac gcggagcgaa agtatccgcg
      421 tgttgatacc gggttttgaa cagcaccacc acatattcac aacgaattgt ggactgtgca
      481 ccagtttggc cttggagagt cgttaccgac agtgtgtcca tccttggagg gcggcaaatg
      541 taattggtcg atcggaattc cgcactcatc ccctcacttt atgggcaagc atgggtacaa
      601 aactgaacga cccatgctcc tgacgatcaa agacatcagc cccaattttg gaccaggacc
      661 acattttctg acgaccatcc ggcccgaccg ggatcaccag gatacccccc agcttcaact
      721 ggccaagcaa tgcctccgga acctctggcg ctccgcaggt tacaatgatc cgatcgaagg
      781 gaccatgcag gggagcacct ttgtatccat cgccgtgcat cagggtggcc ttgtagccca
      841 accggaccag tcgctgcttg gtggtctcga acaacggctt gtgccgttca atgctgaaga
      901 ccttcgcccc cagttcgcac agcacggcgg tctggtaccc gctaccggtg cctatctcca
      961 gcaccttcat gcccggctcc acccgaagta actggctttg gacagccacc gtggtgggtt
     1021 gcgaaatggt ttggccgcaa ccaatgggaa acgcctggtc ggcgtacgcc agggacaaga
     1081 acgcgctgtc gtcgatgaac agatgacgcg gtacggctcc cattgcacg
//
LOCUS       DAOQOC010001196         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_125089_length_1127_cov_2.549440, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001196 DAOQOC010000000
VERSION     DAOQOC010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125089_length_1127_cov_2.549440"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..559)
                     /locus_tag="PL106_15480"
     CDS             complement(29..559)
                     /locus_tag="PL106_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34530.1"
                     /translation="MAGKKQDLLDHAFAALLSDDDAKVLAAIAYVQERGDARAIMPLL
                     HALARTSDQHRQQKIHELLYEVKVKDAAAELVKALDEPELRGVRQTVIATFWNAGLDA
                     APHTEKLVACAVEGTAEECFECLTVLENQEVLPEKAVLQGIKLVNAAIASNTDEYKGV
                     MLGSLLVELKARVGKD"
     gene            complement(611..895)
                     /locus_tag="PL106_15485"
     CDS             complement(611..895)
                     /locus_tag="PL106_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34531.1"
                     /translation="MSGTTLARQLRGLHRTVLMLETELRHGRVDEELIAGIDAQMERG
                     IATAHGCEGLRALVDALRESTLTPRAELLSDTIRGCGKLKDAIQGVLEQL"
BASE COUNT          186 a          368 c          349 g          224 t
ORIGIN      
        1 ccggatgatc cactgaacag ggtagcattc aatccttgcc cacccgcgcc ttcagctcca
       61 ccagcaggct cccgagcatc acgcccttgt actcgtcggt attggaagcg atggcggcat
      121 tcacgagctt gatcccctgg agcacggcct tctcgggcag cacctcctgg ttctccagca
      181 cggtgaggca ctcgaagcac tcctcggcgg tgccttccac ggcgcaggcc accagttttt
      241 cggtatgtgg tgcggcgtcc agacctgcgt tccagaaggt ggcgatcacc gtttggcgca
      301 ccccgcgcag ctccggctcg tccagggcct tcaccagttc ggcggcggcg tccttcacct
      361 tcacctcgta cagcagctcg tggatcttct gctggcggtg ctggtcgctg gtccgggcca
      421 gggcgtgcag caggggcatg atcgcgcggg catcgccacg ttcctgcacg taagcgatgg
      481 cggccagcac cttggcgtcg tcgtcgctca aaagcgcggc aaaggcgtgg tccagcaggt
      541 cttgtttctt tccggccatg ggcccgtgaa ggtagggggc tgcgtcaagg tcagcattgg
      601 agcggcccaa ctacagctgt tccaagaccc cttggatcgc gtccttcagt ttgccacagc
      661 cccggatggt atcgctcagc aattccgcgc gcggggtgag ggtgctctcg cgcagcgcat
      721 ccaccagcgc acgcaggcct tcgcaaccgt gagctgtagc gatcccccgc tccatctgtg
      781 cgtcgatccc ggcgatcaat tcctcgtcca cccggccgtg acgcaactcg gtctccagca
      841 tcagcacggt ccggtggagg ccgcgtaact ggcgggcgag ggtagtaccg gacatggggc
      901 gaatatcggg gtgtgcatcg tttctgatcg atgaccgtcg tcgttcctgc ttttccgacc
      961 tggatccgtc gatcactaca aggcatgcgg cgatcaatcg aagcgtgaag agcgacgcga
     1021 aaggagcgcc ttctacattg ggccccatgc gaacattgta caggctattg ggcatcgccg
     1081 cgttgtgggt gaccatgatg gaccccgcgt cggcccaagg acccgcg
//
LOCUS       DAOQOC010001197         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_125106_length_1127_cov_2.411381, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001197 DAOQOC010000000
VERSION     DAOQOC010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125106_length_1127_cov_2.411381"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PL106_15490"
     CDS             <1..302
                     /locus_tag="PL106_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34532.1"
                     /translation="LGHWNAEEWFTKNPPVIRPEFVEHTLDDIEAVLNVIEKQNGLGP
                     WVIGGGPSPMGGAEDLLSCLQTQKDVWDEQDRKREEEREKYRAEREKRRAMEAKP"
     gene            322..1056
                     /locus_tag="PL106_15495"
     CDS             322..1056
                     /locus_tag="PL106_15495"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNA34533.1"
                     /translation="MATRKRLRRIVEEVYHIPYYGGAEKRFGGYLNEIRAFLNEQRFP
                     NEKTYLTECDRIAKLVEDIGSFMFNEDEGKHHNKVQLAMWHLTRRILAENPKIFVVHG
                     RNVDVRNAVTSVLGKLKLDFEVLEDRNNSGATVVEKFLECAEECDFAIVLMTADDEGG
                     LRGETQRSLRARQNVILELGYFIRHVGRKNIIIMHEDGVHIENPTDLSGIVHIHYDRS
                     GGWKRSVLKDMEAAGIYIHPPYARTV"
BASE COUNT          278 a          284 c          358 g          207 t
ORIGIN      
        1 atctcggtca ctggaacgct gaggagtggt tcacaaagaa cccgccagtg atacggcctg
       61 agttcgtgga gcacaccttg gatgacattg aggccgtgct caacgtgatc gaaaagcaga
      121 acggacttgg gccgtgggtc attggtggtg gaccatcgcc catgggcgga gccgaagatc
      181 tgctgtcctg cttgcaaacc cagaaggatg tctgggacga acaggatcgc aagcgcgagg
      241 aagaacggga gaagtaccgc gctgaacgtg agaagcgaag ggcaatggag gccaagccat
      301 agagatcctt cactacatca catggccacg aggaaacgcc tgcgcaggat cgttgaggaa
      361 gtctatcaca tcccgtacta cggcggtgcg gagaagcgct ttgggggtta tctcaacgag
      421 atccgtgcgt tcctgaacga gcagcggttc ccgaatgaaa agacgtacct gaccgaatgt
      481 gatcgcatcg ccaagctggt ggaggacatc gggagcttca tgttcaacga agacgagggc
      541 aaacatcaca acaaggtgca gcttgccatg tggcacttga cacgccgcat tctcgctgag
      601 aacccgaaga tcttcgtggt acatggccgc aacgtggacg tgcggaacgc ggtgacctcg
      661 gtacttggaa aattgaagct ggactttgag gttctcgaag accgcaacaa ctcaggggcc
      721 acggtcgtgg agaagttctt ggaatgtgcg gaagagtgcg acttcgccat tgtgctcatg
      781 acagcggatg acgaaggcgg tctgaggggc gaaacgcagc gctcactacg ggcacgccag
      841 aacgtgatcc ttgaactcgg ctacttcatc aggcatgtcg ggcgcaagaa catcatcatc
      901 atgcacgagg acggcgttca tattgagaac ccgaccgacc tgagcgggat cgtgcatatc
      961 cattacgatc gaagcggcgg atggaagcgg agcgtgctga aggacatgga agcggctggc
     1021 atctacatcc acccaccgta cgcccgtacc gtttaggcgt ggcgtgcttg ggatgcgtcc
     1081 cgacttgccg ctgacatgcg gcagaacgtc atggagttgt agggaag
//
LOCUS       DAOQOC010001198         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_125265_length_1126_cov_3.335201, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001198 DAOQOC010000000
VERSION     DAOQOC010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125265_length_1126_cov_3.335201"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PL106_15500"
     CDS             complement(<1..718)
                     /locus_tag="PL106_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34534.1"
                     /translation="MSTEVPPLVPATRHVAERTTYSVLFAIAFSHLLNDTIQSLIPAI
                     YPVVKGSFNLSFAQVGLITLTFQLTASILQPLVGHWTDRKPQPFALVGGMVFTLFGLL
                     ALAWAPSFGTILLAVGLVGVGSSIFHPEASRMAYVASGGKRALAQSVFQLGGNSGSAL
                     GPLLAAAIIVPFGRTHTAWFSVLPLIAMVVLWQVGRWYLRMIAIRAHRQARRPAEQPV
                     RLPRRRVIGIVGVLLLLIFSK"
     gene            complement(761..>1126)
                     /locus_tag="PL106_15505"
     CDS             complement(761..>1126)
                     /locus_tag="PL106_15505"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNA34535.1"
                     /translation="NAACEALKAAGAKRALVLPVGGAFHSPLMEPARAELATAIAYTP
                     IVDPRCPVYQNVDARPHSDPARIKANLIAQLTAPVRWTRIMTNMLADGATKAVEVGPG
                     NVLQGLFKKVNKDLETASA"
BASE COUNT          219 a          365 c          359 g          183 t
ORIGIN      
        1 gtttcgagaa gatcagcaac aggaggaccc ccacgatccc gatgacccgg cgacggggca
       61 agcgcacggg ttgttccgcc ggtcgccggg cctgtcggtg cgcgcggatg gcgatcatgc
      121 gcaggtacca gcgccccacc tgccagagca ccaccatggc gatcaacggc agtacactga
      181 accacgccgt gtgcgttctt ccgaacggaa cgatgatcgc cgccgccaac aacggaccca
      241 gggccgagcc cgagttgccg cccagctgga acactgattg cgccaaggcc cgcttgccgc
      301 cagaggccac atacgccatg cgcgatgcct ccggatggaa gatggacgac cccacaccca
      361 ccagccccac cgccagaagg atggtgccga aactcggcgc ccacgcaagg gccagcagtc
      421 cgaacagggt gaagaccatc ccgcctacga gggcgaaagg ctgcggcttg cgatcggtcc
      481 aatgccctac caacggttga aggatggacg ccgtgagctg gaaggtgagc gtgatcagac
      541 ccacctgcgc gaaactcagg ttgaacgatc ccttaaccac gggatagatc gctggaatga
      601 gcgactggat cgtgtcgttc agcaggtgcg agaaggcgat agcgaaaagc acggagtacg
      661 tcgtgcgctc agccacatga cgcgtggcgg ggaccagggg agggacctcg gtgctcatgc
      721 tccgttgtcc tgcgctgctc ttgatgggca caggtccgat ctacgccgac gccgtttcca
      781 ggtccttgtt caccttcttg aacaaccctt gcaacacgtt gccgggtccc acttccaccg
      841 ccttggtcgc accatcggcc agcatgttcg tcatgatccg cgtccagcgt acgggcgcgg
      901 tgagctgtgc gatcaggttg gccttgatgc gcgcgggatc gctgtgcggc cgggcgtcca
      961 cgttctggta taccggacaa cgaggatcaa cgatgggcgt gtacgcgatg gccgtggcca
     1021 gctcggcccg tgcgggttcc atcagcggac tgtggaaggc cccgcccacc ggcagtacca
     1081 gtgcacgctt ggcccctgcg gctttgagcg cttcacaggc ggcatt
//
LOCUS       DAOQOC010001199         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_125421_length_1126_cov_1.881419, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001199 DAOQOC010000000
VERSION     DAOQOC010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125421_length_1126_cov_1.881419"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PL106_15510"
     CDS             complement(<1..159)
                     /locus_tag="PL106_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNA34536.1"
                     /translation="MKVFKFGGASVKDAQGVRNVARVLEAFKQDDLLIVVSAMGKTTN
                     ALEEVVKAY"
     gene            complement(285..>1126)
                     /locus_tag="PL106_15515"
     CDS             complement(285..>1126)
                     /locus_tag="PL106_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNA34537.1"
                     /translation="LSNAEQMGEGWSDWMAMMMTMRPGDAATTSRGMGTFVRDQANDG
                     IGIRPAPYSTDPAINNYTYAATNDDAISEPHGIGFIWATMLWDLNWAFIDTYGVDADL
                     YNGTGGNNMAIQLMMDGLKLQPCSPGFVDGRDAILRADTLNYGGANACLIWNAFAHRG
                     LGYSADQGDPYSRYDQTEAFDLPPGCSVGINTVSARRPVPVLVPNPANDHVTMSLDRA
                     PDQTAILRIHDGAGRMVRSLPWPAGTVRMELDLGDLAPACYTIGVDAGGYPVRARLVV
                     SGR"
BASE COUNT          187 a          376 c          335 g          228 t
ORIGIN      
        1 gtaggccttc accacctctt ccagcgcatt ggtggttttg cccatggcgc tcaccacgat
       61 gaggaggtca tcctgtttga acgcctccag cacgcgcgcc acattgcgca cgccttgtgc
      121 atccttcacg ctggcccccc cgaacttgaa cactttcatt tcctctggtt gaattcgcgg
      181 cgtgaatatc cgtcagcgcg ttcatgtcgt gatccggtca gggcggaccg gtgatcctcg
      241 aacactttgt gtgtccggaa cctccgcatg gatccgttcc gcgcttatcg cccgctcact
      301 accaggcgtg cacgtaccgg atagcctccc gcatccactc cgatcgtata gcacgccggc
      361 gccagatcgc ccaagtcgag ttccatgcgc acggtgcccg caggccacgg caacgaccgc
      421 accatccgac ccgcaccgtc gtggatgcgc aggatggccg tctggtcagg cgcccgatcc
      481 aggctcatgg tcacgtggtc gttcgccggg ttcggcacca ggacgggtac cggcctcctg
      541 gccgatacgg tattgatacc tacgctgcat ccgggcggca ggtcgaaggc ctcggtctgg
      601 tcgtaccgcg aatacggatc gccctgatcg gcgctgtacc cgagaccccg atgggcgaat
      661 gcgttccaga tcaggcaggc gttggcgcca ccgtagttca acgtgtcagc acggaggatg
      721 gcatcccggc catcgacgaa gcccggcgag cacggttgca gcttcaagcc gtccatcatc
      781 agctggatgg ccatgttgtt gccgcccgtt ccgttgtaca gatcggcatc cacaccgtac
      841 gtatcgatga aagcccagtt caggtcccag agcatggtgg cccagatgaa gccgataccg
      901 tgcggctccg aaatggcgtc gtcgttggtg gccgcgtagg tataattgtt gatcgccggg
      961 tccgtggaat acggggctgg ccggatgccg atgccgtcgt tcgcctggtc ccggacgaag
     1021 gtgcccatgc cgcggctcgt ggtggccgca tctcccggtc gcatggtcat catcatggcc
     1081 atccagtcgc tccagccctc gcccatctgc tcggcgttcg acaggc
//
LOCUS       DAOQOC010001200         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_125512_length_1125_cov_3.310280, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001200 DAOQOC010000000
VERSION     DAOQOC010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125512_length_1125_cov_3.310280"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PL106_15520"
     CDS             <1..327
                     /locus_tag="PL106_15520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA34538.1"
                     /translation="IFCFPSWFEAESFGLVLVEAMMFAKPVVTTIWRGIPSVAQNGVN
                     GYTVAVRDPAATADRLLRLAHDPALRQRMGLEGRRIFEERFTLERFHRNMEDALARCA
                     NTIEKE"
     gene            324..>1125
                     /locus_tag="PL106_15525"
     CDS             324..>1125
                     /locus_tag="PL106_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA34539.1"
                     /translation="MIANARLLITGGSGFIGTNTVAHFLAQGTEVLNLDWNPPLDPVH
                     RTYWKECDILDEGRMRDIFQQFKPTHVIHLAARTDTDIQDDIRAYEQNHTGTENVLRV
                     VKASPSIERLIVTSTQFVCEAGYQPKNDQDFKPFTLYGETKRRCELATRAAGLTCAWT
                     IIRPTTIWGPYSLRYRDVMFKVMRKGMYFHPGGRKTIRSYGYVGNVVWQIDRMLTLPK
                     EDMNGRVFYVGDQPIDLRVWVEAVSRRLTGKPVRYIPGPVVRAIALCGD"
BASE COUNT          232 a          346 c          354 g          193 t
ORIGIN      
        1 atcttctgct tcccgagctg gttcgaagcg gagagcttcg ggctcgtgct ggtggaggcc
       61 atgatgttcg ccaagcccgt ggtcaccacc atctggcggg gcatcccatc cgtggcccag
      121 aacggtgtga acggatacac cgtggccgtg cgcgacccgg ccgccacggc cgaccggctg
      181 ctgcgccttg cgcacgaccc cgccctgcgg caacgcatgg gccttgaagg gcgacgcatc
      241 tttgaagaac gattcacgtt ggaacggttc caccggaaca tggaggatgc gctcgcccgg
      301 tgcgcgaata ccatcgaaaa ggaatgatag cgaacgcgcg gctgctcatc acgggaggat
      361 ccggattcat cggcaccaac acggtggcgc atttcctggc acaagggacc gaggtgctca
      421 acctggactg gaacccgccc ctggacccgg tgcaccggac gtactggaaa gaatgcgaca
      481 tcctggatga gggtcgcatg cgggacatct tccagcagtt caagcccacg cacgtgatcc
      541 atctcgcggc acggaccgat accgacatcc aggacgacat cagggcgtac gagcagaacc
      601 ataccggcac ggagaacgtg ctgcgcgtgg tgaaggcctc accttccatc gaacggctca
      661 tcgttaccag cacccagttc gtgtgcgaag cgggttacca gcccaagaac gatcaggact
      721 tcaagccgtt cacgttgtac ggcgaaacga agcgccgctg cgagttggcc actcgggccg
      781 ccggactcac gtgcgcttgg acgatcatcc gtcccacaac gatctgggga ccgtacagtc
      841 tacggtaccg cgatgtgatg ttcaaggtga tgcgcaaagg gatgtacttc cacccgggcg
      901 ggcgcaaaac catccgatca tacgggtatg taggcaacgt ggtctggcag atcgaccgca
      961 tgctcacgct gcccaaggag gacatgaacg gtcgggtgtt ctacgtgggc gaccaaccga
     1021 tcgatctgcg ggtgtgggtt gaagccgtat cgcggcgcct caccggaaaa ccggtacgct
     1081 acatccccgg tccggtggtg cgtgcgatcg ccttgtgcgg cgatg
//
LOCUS       DAOQOC010001201         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_125950_length_1123_cov_5.620787, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001201 DAOQOC010000000
VERSION     DAOQOC010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125950_length_1123_cov_5.620787"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PL106_15530"
     CDS             complement(<1..288)
                     /locus_tag="PL106_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA34540.1"
                     /translation="MSTSILWADDEIDLLKPHVLFLEQKGYTVDTVNNGLDAVDLVGK
                     REFDIVFLDENMPGLSGLETLERIKGMAPRTPVIMITKSEEERIMEEAIGSK"
     gene            425..>1123
                     /locus_tag="PL106_15535"
     CDS             425..>1123
                     /locus_tag="PL106_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA34541.1"
                     /translation="MSTPGKILNDPIYGFITVPHPVVQRLIDHAWFQRLRYIKQLGLG
                     HLVYPGALHTRFHHALGAMHLMSEAVATLRAKGHSITDEEALGAHVAILLHDIGHGPF
                     SHALEHSIVEGMDHEAISALVMDALNAEFDGALATGIRIFRDQHPKRFLHQLVSSQLD
                     VDRIDYLNRDSFYTGVSEGVIGGERIIKMLQVVNDRLVVEEKAIYSIEKFIVARRLMY
                     WQVYLHKTVVACEMM"
BASE COUNT          205 a          353 c          339 g          226 t
ORIGIN      
        1 cttgctgccg atggcctcct ccatgatgcg ttcctcctcg ctcttcgtga tcatgatcac
       61 cggggtgcgc ggggccatgc ccttgatgcg ctccagcgtt tcgagcccgc tgaggcccgg
      121 catgttctcg tccaggaaca cgatgtcgaa ttcgcgtttg cccaccaggt ccacggcgtc
      181 aagaccattg ttcaccgtat ccacggtgta gcccttctgc tccaggaaga ggacgtgggg
      241 tttcaggagg tcgatctcgt catcggccca gaggatgctc gtgctcatgc gttggttcgg
      301 ttttcccttc ggaacgtgac gggaaagccg tctattttcg gtgcgtgaaa ttaggccgat
      361 tccacccgct gcgcccgatc attgggcgaa agccctggaa gccgcgccag gactgacgat
      421 ccgcgtgtcc actcccggca agatcctcaa cgaccccatc tacgggttca tcaccgtgcc
      481 ccacccggtg gtgcagcggt tgatcgacca tgcgtggttc cagcggctcc gctacatcaa
      541 gcagttgggg ctgggccacc tggtgtaccc cggcgcgctg cacacccgct tccaccacgc
      601 gctgggcgcc atgcacctga tgagcgaggc cgtggccacc ctgcgcgcca agggccacag
      661 catcaccgat gaggaggcct tgggcgcgca tgtggccatc ctgctgcacg acatcggcca
      721 cggaccgttc agtcacgcac tggaacacag catagtggaa ggcatggacc acgaggccat
      781 cagtgccctg gtgatggatg ccctcaacgc tgaattcgat ggtgcgttgg ccacgggcat
      841 ccgcatcttc cgcgatcagc accccaagcg cttcctgcat cagctcgtga gcagccagct
      901 tgatgtggac cgtatcgact acctgaaccg cgacagtttt tataccgggg tgagcgaggg
      961 tgtcatcggc ggtgaacgca tcatcaagat gctgcaggtg gtgaacgacc gcctcgtggt
     1021 ggaggaaaag gccatctaca gcatcgagaa attcatcgtg gcgcgccgcc tgatgtactg
     1081 gcaggtgtac ctgcacaaga ccgtggtggc ctgcgagatg atg
//
LOCUS       DAOQOC010001202         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_126107_length_1123_cov_2.093633, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001202 DAOQOC010000000
VERSION     DAOQOC010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126107_length_1123_cov_2.093633"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PL106_15540"
     CDS             complement(<1..695)
                     /locus_tag="PL106_15540"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305034.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNA34542.1"
                     /translation="MSDQLTKRAQDHAQWYNDLVLKADLAEHSDVRGCMVIKPHGYAI
                     WEKMQRALDGMFKDTGHVNAYFPLFIPKSYLAKEASHVEGFAKECAVVTHYRLKSDPE
                     HGVVVDPEAKLEEELIVRPTSETIIWNTYRGWIKSYRDLPLLINQWANVVRWEMRTRL
                     FLRTAEFLWQEGHTAHATKAEAVEETERMLDVYARFAEEWLAMPVIKGVKTASERFAG
                     AEETYCIEAMMQDG"
     gene            787..>1123
                     /locus_tag="PL106_15545"
     CDS             787..>1123
                     /locus_tag="PL106_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34543.1"
                     /translation="MRTTIRLFSAALLLGACGTVRETAQVRDDVYDIPDRSVVASAHG
                     GGSTSDVGTAQASADEDYYNEQDASQYAQGSYYDRTYNDPYWSRNRFGFGYGGGYGGM
                     YGGSSFGYGY"
BASE COUNT          193 a          350 c          338 g          242 t
ORIGIN      
        1 ccgtcctgca tcatcgcttc gatgcaatag gtctcctcgg ccccggcgaa gcgctcactg
       61 gcggtcttga cccccttgat caccggcatg gccagccact cctcggcgaa gcgggcgtac
      121 acatccagca tacgctcggt ctcctccacg gcctcggcct tggtagcgtg cgccgtatgc
      181 ccttcctgcc acaggaattc ggccgtgcgc aggaacaggc gcgtccgcat ctcccatcgc
      241 accacgttgg cccattggtt gatcagcaag ggcaggtccc tgtagctctt gatccagccg
      301 cggtacgtgt tccagatgat ggtctcgctc gtggggcgca cgatgagttc ctcctccagt
      361 ttcgcttccg ggtccaccac cacgccgtgc tcgggatcgc ttttcagccg gtaatgcgtt
      421 accaccgcgc attccttggc gaagccctcc acgtggctgg cttccttggc caggtagctc
      481 ttggggatga agagcgggaa gtacgcgttc acgtgcccgg tgtccttgaa catgccgtcc
      541 agcgcacgct gcattttttc ccagatagcg taaccgtggg gcttgatcac catgcagccg
      601 cgcacgtcgc tgtgctcggc cagatcggcc ttcaggacca ggtcgttgta ccattgcgca
      661 tggtcttgtg cacgttttgt cagctgatcg ctcattcggc ctgttcttgg tattagcttt
      721 gatgcaagaa cgcgcaagga tacggctaag ccccggccct ccggggggat caacaaccga
      781 gcgaacatga gaaccacgat caggcttttt tccgcggccc tgttgttggg cgcctgtggc
      841 accgtgcgag agacggcaca ggtgcgggat gatgtgtacg acattccgga ccggagcgtg
      901 gtggcctcgg cgcatggagg cggttccacc tctgacgttg gcaccgcaca ggcctcggcg
      961 gacgaggact actacaacga gcaggacgca tcgcagtacg cacagggcag ttattacgac
     1021 cgcacgtaca acgatccgta ctggagcagg aaccgattcg gtttcggata cggtggcggc
     1081 tatggtggta tgtacggtgg ctcatccttc ggctacgggt aca
//
LOCUS       DAOQOC010001203         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_126110_length_1123_cov_2.058989, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001203 DAOQOC010000000
VERSION     DAOQOC010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126110_length_1123_cov_2.058989"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1123
                     /locus_tag="PL106_15550"
     CDS             25..>1123
                     /locus_tag="PL106_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA34544.1"
                     /translation="MANFLWSMRHEPGITAEDRLVAVTTLSFDIAVLELMLPLYNGAE
                     VILADRDTAMDGERLSALLSESRATMMQATPATWRVLVDAGWRGTPGFRALCGGEGLP
                     ATLAKAILERSTELWNMYGPTETTIWSTCTRVEHPERGIHIGRPIANTTVWILDAHDQ
                     PTPSGVPGEICIGGEGVALGYLDRPELTAERFVTLPINGVDTLLYRTGDRGRWRNDGT
                     LEHLGRLDFQVKVRGYRIELGEIEARCSEAAGVGRCVVITREDQPGDVRLATYLVLSE
                     PARYDQAALRKHLGATLPQYMVPQHIVVLDELPLLPNGKIDRKALPKPESDQRATRTV
                     QEHQGPRDAIEQTLTSIWSTALGVDKISINDD"
BASE COUNT          216 a          345 c          382 g          180 t
ORIGIN      
        1 ggtgttcgcc ttccgcaccg cgctgtggcc aacttcctct ggtccatgcg gcacgaaccg
       61 ggcatcacgg ccgaggaccg cttggtcgcg gtgaccacgt tgtcgttcga catcgcggtg
      121 ttggaactga tgcttccact gtacaacggg gcggaagtga tccttgcgga cagggatacg
      181 gcgatggacg gggaacgcct gtccgcgttg ctgtccgaga gcagagccac catgatgcag
      241 gccacaccgg ccacctggcg ggtgttggtg gatgcgggtt ggcgcggcac gcccggcttc
      301 cgtgcgctgt gcggtggcga ggggctgccc gcaacgctgg ccaaggcgat cctggaacgc
      361 agcaccgaac tgtggaacat gtacgggccg accgagacga cgatctggtc cacgtgcacg
      421 cgcgtagagc atccggaacg gggcatccat atcgggcgac cgatcgcgaa caccacggtc
      481 tggatactgg acgcccatga ccagcccact ccttcgggtg taccggggga gatctgcatc
      541 ggcggcgaag gcgtggccct cggctacctg gatcgtccgg agctgaccgc tgaacgattc
      601 gtgacgttgc cgatcaacgg cgtggacacc ctgctctacc gcacgggcga ccggggccgt
      661 tggcgcaacg acggaacact ggagcatctg gggcggctgg acttccaagt gaaggtgcgc
      721 ggctaccgga tcgagttggg cgagatcgag gcgcgctgca gcgaagcggc cggggtgggg
      781 cgttgcgtgg tgatcacgcg cgaggaccag cccggagacg tacggctcgc aacgtacctg
      841 gtgctgagcg aaccggcgcg atatgatcag gctgccctcc gcaaacacct cggtgccaca
      901 ctgccgcaat acatggtacc gcagcacatc gtggtactcg atgaattgcc actgcttccg
      961 aacggcaaga tcgatcgcaa ggcactgccc aaacccgaga gcgaccagcg cgcaacgcga
     1021 acggtacagg agcatcaggg gccacgcgat gccatcgagc agaccttgac ctccatctgg
     1081 agcaccgcgc tcggggtcga taagatcagc attaacgatg att
//
LOCUS       DAOQOC010001204         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_126210_length_1122_cov_3.595127, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001204 DAOQOC010000000
VERSION     DAOQOC010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126210_length_1122_cov_3.595127"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1122
                     /locus_tag="PL106_15555"
     CDS             89..>1122
                     /locus_tag="PL106_15555"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNA34545.1"
                     /translation="MTHLPHTMPALLLTTTLVTACVTSAPVAGNGPDYWSPQELVCED
                     RVYSPTIHTVQFFKKGFELAPPVIELGAQDPLVLRFDDLQPNPEQLTYSIVHCDAQWR
                     PSDLMTNQYITGSPTDYVDPAEQSRMTLQPFIEYTLELPNEFMRPAVAGNYLLKVYRN
                     NDPDDLVLTRRFLVFEQRLDVQASIQASRDVERRDIAQQLDLTIRHPGITIRDPFSDI
                     SVTVLQNMRWDDARTGFKPKFLRDNELVYDHPKEGLFLGGSEWRTYDLKNTRYVTNEI
                     KVIAHSPDGLLEVFLLPDVKRNIRVYVQEPDLNGKLFVRNDQMDGDPLGADYTWVNFT
                     LPLDAPVASGG"
BASE COUNT          233 a          384 c          308 g          197 t
ORIGIN      
        1 agtccgccct cggcggactt ttcattccac gtggtccgtt cggtatgaac ggcaccgtat
       61 tggcccggat cccccgctga tcaagccgat gacgcacctc ccccacacca tgcccgccct
      121 gctcctcacc accaccctgg tcacggcctg cgtcacctcc gcgccagtgg cgggcaacgg
      181 ccccgactac tggtctccac aggaactggt atgcgaggac cgggtgtaca gccccaccat
      241 acatacggta caatttttca agaaaggctt cgaactggcg ccaccggtga tcgaactggg
      301 cgcgcaggac ccgctggtac tgcgtttcga tgatctgcaa cccaaccccg aacagctcac
      361 ctattccatt gtccattgcg acgcgcaatg gcggcccagc gacctgatga ccaaccagta
      421 catcacgggc tcgcccaccg actatgtgga ccctgccgag cagagccgta tgaccctgca
      481 gcccttcatc gaatacacgt tggaacttcc caacgaattc atgcgtccgg ccgtggcggg
      541 caactacctg ctgaaggtgt accgcaacaa cgacccggac gacctggtgc tcacccggcg
      601 cttcctcgtc ttcgagcagc ggctcgacgt acaggccagc atacaggcca gccgcgatgt
      661 ggagcgccgc gacatcgccc agcaactgga tctcacgatc cgacacccgg gtatcacgat
      721 acgtgatccg ttcagcgata tcagtgtaac cgtgctccag aacatgcggt gggacgatgc
      781 gcgcaccggc ttcaagccga aattcctacg ggacaatgaa ctggtgtacg accaccccaa
      841 ggagggcctc ttcctgggcg gcagcgagtg gcgcacttac gacctgaaga acacgcggta
      901 cgtgaccaac gagatcaagg tgatcgccca ctcgcccgac gggctgctcg aggtcttcct
      961 gttgcccgat gtgaaacgga acatccgggt gtacgtgcag gagccggacc tcaacggcaa
     1021 gcttttcgta cgcaacgacc agatggacgg ggatcccctc ggagccgact acacctgggt
     1081 caatttcacc ctgccgcttg atgccccggt ggccagtggc gg
//
LOCUS       DAOQOC010001205         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_126216_length_1122_cov_3.470478, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001205 DAOQOC010000000
VERSION     DAOQOC010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126216_length_1122_cov_3.470478"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PL106_15560"
     CDS             complement(<1..603)
                     /locus_tag="PL106_15560"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNA34546.1"
                     /translation="MKQILAPMAVLSLPLVSNAQDLPALSPHAEVEQIIGLTDVEVEY
                     SRPSARGRAIFGDLVPYDEIWRTGANKSTKITFSGSVDFGGKPVKAGSYSLFAIPHEG
                     VWELILNSNPELNGAFDRKEAEDVLRTKAEVQYGEFVETFTIGFANLGQDKADLELAW
                     EKQRVRITISADATAQGLVNIKEALAKSDAGYSAYNRAASF"
     gene            complement(861..>1122)
                     /locus_tag="PL106_15565"
     CDS             complement(861..>1122)
                     /locus_tag="PL106_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNA34547.1"
                     /translation="IKQYVKLFEMDKVKVSFDKKVLDFIVEKAIEYKLGARGLRSICE
                     AIMTDAMYEIPGAAEKPSELRVTLTYARDKFERSRVGQLKVA"
BASE COUNT          219 a          343 c          321 g          239 t
ORIGIN      
        1 gaacgaggcc gcgcgattgt acgcactgta ccccgcatcg ctcttggcca gcgcttcctt
       61 gatgttcacc agtccttgtg ccgtggcatc cgcactgatg gtgatgcgca cgcgttgctt
      121 ctcccatgcg agttcgagat cggccttgtc ctgacccagg ttggcgaagc cgatggtgaa
      181 ggtctccacg aactcaccgt actggacttc cgctttcgta cggagcacat cctcggcctc
      241 cttgcggtcg aaggccccgt tgagttccgg gttgctgttc aggatcagtt cccatacacc
      301 ctcgtgcggg atggcgaaga gcgagtaact tccggctttg acaggctttc caccgaaatc
      361 cacggacccg ctgaaggtga tcttcgtgct cttgttggct ccggtgcgcc agatctcgtc
      421 gtaggggacc agatcgccga agatggcacg ccccctggcg gatgggcgcg agtattcaac
      481 ctccacatcg gtaagaccga tgatctgctc cacctcggcg tggggactga gcgcgggaag
      541 gtcctgtgcg ttggagacca gaggaaggga aagcaccgcc atgggcgcga ggatctgttt
      601 catgatgcgt tgtgtaaggg gcgacaaagc tagcccatcc tgcttcaccg gatccgggag
      661 tgccggccat gatcaacggg tgtgccgtgt atcatcctcc tcgggatccc catccggaca
      721 tcgctcctgt acgccgtccg acaccctggc caaccaggtc cgatcacgcg aaggaacatc
      781 aaccctgaac aagcatgggc cgcacacgat caacaatgat ccaactggcc aacgatgcgc
      841 acgcatcctg acgggaaggc ttatgccact ttcaactgcc caacgcggct gcgctcgaat
      901 ttgtcgcgtg cataggtcag tgtaacgcgc aactcagaag gtttctcggc ggcgcccgga
      961 atctcgtaca tcgcgtcggt catgatggcc tcacaaatgg agcgcagacc acgtgcgccg
     1021 agtttgtact cgatggcctt ctccacgatg aagtcgagca ccttcttgtc gaaggagacc
     1081 ttcaccttgt ccatctcgaa gagcttcacg tactgtttga tg
//
LOCUS       DAOQOC010001206         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_126468_length_1121_cov_3.294559, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001206 DAOQOC010000000
VERSION     DAOQOC010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126468_length_1121_cov_3.294559"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PL106_15570"
     CDS             complement(<1..592)
                     /locus_tag="PL106_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA34548.1"
                     /translation="MDNTINRRDFIKHTTTVTVGMGLGAGALLAACGSGGAGDAAQAD
                     AAPAAGTDNSAPAAMAKRMLGALEVTELGLGCMNIAWAYGPSTGKDASIALVRKAYEE
                     GIRFFDTAEIYGPFISEQIVGEALEPYRNEVVIASKIGFDIDFETGEQHGLTSRPEHI
                     RLAVEQMLKRLRTDRIDLLYQHRVDPQVPIEEVAGAM"
     gene            889..>1121
                     /locus_tag="PL106_15575"
     CDS             889..>1121
                     /locus_tag="PL106_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34549.1"
                     /translation="MLTPYHAKYFAHELTRLGGSGVERLGRSLFDASVQLQPHQIDAA
                     LFALKSPLEQGVMLADEVGLGKTIEAGLVICQL"
BASE COUNT          176 a          370 c          351 g          224 t
ORIGIN      
        1 ccatggcccc ggccacctcc tcgatcggca cctgcgggtc cacgcggtgc tggtagagca
       61 ggtcgatgcg atcggtgcgc agccgcttga gcatctgctc cacggcaagg cggatgtgct
      121 ccggcctgct ggtcaagccg tgctgttcgc ccgtctcgaa gtcgatatcg aaaccgatct
      181 tggaggcgat gaccacttca ttgcggtagg gttccagggc ctcgcccacg atctgctcac
      241 tgatgaacgg cccgtagatc tcggccgtat cgaagaagcg gatgccttcc tcataggcct
      301 tgcgcaccag cgcgatggaa gcatccttgc cggtggacgg cccgtaggcc cacgcgatgt
      361 tcatgcagcc caggcccagc tccgtcactt ccaatgcgcc gagcatgcgc ttggccatag
      421 cggccggggc gctgttgtcc gtaccagcgg ccggtgcggc atccgcctgt gcagcgtcgc
      481 ccgcgccccc gcttccgcag gcggccagca acgcgcccgc gcccaggccc atgcccacgg
      541 tcaccgtggt ggtgtgcttg atgaagtccc ttcggttgat cgtgttgtcc atggtcagtg
      601 gggttcggtg aggaggtttg cgctatgatc ctgcgcacgt ccatcctgga cggccacaca
      661 aaattccacc gcacggccct gctgccgcta tcgcgatccc tacgaagact tgcgaaattc
      721 aaacagcccg gaactgcttc ggggctcatc cgatcccgct tgatcggtag tgggcttgat
      781 ggagcgggct tggtcaagat ccgcgatggc gggcgggcgt gtcgtggaca acgggcggct
      841 gctccgtgtt cctcacgcat ttcagggtcg gccgtatttt cagccccgat gctcacccct
      901 taccacgcca agtacttcgc gcatgaactc acccgcctcg gcggctctgg cgtagagcgt
      961 ttgggccgtt cgctgttcga cgcctcggtg cagttgcaac cacaccagat cgacgccgcc
     1021 ctcttcgcgc tgaagtcccc cctggagcaa ggggtgatgc tggccgatga agtgggcttg
     1081 ggcaagacca tcgaggccgg actggtgatc tgccagcttt g
//
LOCUS       DAOQOC010001207         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_127401_length_1117_cov_3.758004, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001207 DAOQOC010000000
VERSION     DAOQOC010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127401_length_1117_cov_3.758004"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..838)
                     /locus_tag="PL106_15580"
     CDS             complement(74..838)
                     /locus_tag="PL106_15580"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNA34550.1"
                     /translation="MEGGTRLVVDIGNSRMKAGLFRHGRLTRTSTMPLGDVAAIGAFV
                     ASDRPEVVVVGSVASPDPAFANALHAWAPVVEVNGASEGPIRSAYRTRDTLGVDRWAN
                     AVAASLLFPGRPTLAISLGTCLTYDLVSADGEHLGGIISPGFRMRARAMGEFTARLMV
                     VDPPDTPALLGTDTEACLASGTHHGLLAELAHTISGFRHKQPGLAVVLTGGDALRFAR
                     ALESGIFAHPFLTLLGLHALSLPARLASGTSSGPGR"
     gene            910..982
                     /locus_tag="PL106_15585"
     tRNA            910..982
                     /locus_tag="PL106_15585"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:943..945,aa:Phe,seq:gaa)
BASE COUNT          214 a          351 c          377 g          175 t
ORIGIN      
        1 gccggtgttc agcagatcac ccaggccgaa ggcgctgtat ggggagccgg tgctggcgac
       61 ctgcgcctgc gactcaacgg ccggggccag aagaagtacc ggaagccagg cgggcaggga
      121 gcgaaagtgc atgcaggccg aggagggtca gaaaaggatg cgcaaagatg ccgctttcca
      181 aggcccgggc gaagcgcagg gcatcgccgc cggtgagcac cacggccagg ccgggctgct
      241 tgtgccgaaa tccgctgatc gtatgtgcca attccgcgag gagcccatgg tgcgtacccg
      301 aagccaaaca cgcctcggta tcggtaccca atagagcggg tgtgtccggt gggtccacca
      361 ccatgagccg cgcggtgaat tcgcccatgg cacgcgcccg catgcggaag cccggtgaaa
      421 tgatgccgcc gaggtgctcc ccatcggcgg aaacgaggtc gtaggtaagg caggtgccca
      481 aggaaatggc cagggtcggc ctaccgggga acaggaggga ggcagccact gcattggccc
      541 agcggtccac acccagggta tcgcgcgtgc ggtaggcact gcggataggg ccctccgatg
      601 caccgttcac ctccaccacg ggagcccatg catgcaatgc gttcgcgaag gcgggatcag
      661 gtgatgccac actgcccacc accaccacct cgggacggtc cgacgcaacg aaggcaccga
      721 tggcagcgac atcgccgagg ggcatggtgc tggtccgcgt caggcgccca tgacggaaca
      781 gccctgcttt catccgggaa tttccgatgt ccactacgag tcgcgtgccg ccttccatcc
      841 gctgtctata ttcgcggccc gtttcgccgc cgctccgcct tcgggcgggg aacgtgcaaa
      901 gatggtactg gtgccttagc tcagctggta gagcaacgga ttgaaaatcc gtgtgtcccc
      961 agttcgattc tgggaggcac cacgaaaaaa gaccccggcg aaggccgggg tctttgcttt
     1021 ccggggtcca gggatcagcc gttggccgcg cgtccgtgcg cctccagcca ggcgatcacg
     1081 ccgttcaggt gctgctgctt cgccggttcg cggagct
//
LOCUS       DAOQOC010001208         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_127459_length_1117_cov_2.845574, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001208 DAOQOC010000000
VERSION     DAOQOC010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127459_length_1117_cov_2.845574"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..>1117
                     /locus_tag="PL106_15590"
     CDS             152..>1117
                     /locus_tag="PL106_15590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNA34551.1"
                     /translation="MFRRPLILAFLLTIVHTCARAQERMIQAGIFRDRQLTQVIVRGT
                     SSPIEVLIDGKPAGQVGSKDAVRISVQGDRLSASSLDRHFPHGRRITFRSTGRGLLRV
                     GTGKPVIERDYPGSITISTANGALLVVNDVALDDYVAGVVQSEAGNHKGVEYYKLQAV
                     SCRTYAITTARRHINEGFNLCDRTHCQVYKGACRLDSIKKAVRATKNLVVVDADIRPI
                     HATFHSNCGGETTNAEDVWSKPESYLVSALDTFCLHEPHAQWRTDMPRTAWLTYLKDS
                     FGLDTMNAATVEAVTSFDPRCRSLYLDGAHPSISLLKVRADLGPAF"
BASE COUNT          215 a          380 c          336 g          186 t
ORIGIN      
        1 ggccataccg cgaacgcatg atcgatgatc tccgcctgct gcgtgaaaag ctcggtggtc
       61 caggcgcttc ggcacgtacg gcggaactcc tgtggaaaag tctgaagcga cgtgcctgac
      121 cactgcgcct cgccccggta ggttcgcgcc cgtgttccgg cgtcccctca tcctcgcgtt
      181 cctgctgacc atcgtgcata cctgcgcacg cgcacaggaa cgcatgatcc aggccggcat
      241 ctttcgcgac cgccagctca cccaggtgat cgtgcgcggc accagcagcc ccatcgaggt
      301 actgatcgac ggcaagcccg cggggcaggt gggatccaag gatgccgtgc gcatcagcgt
      361 acaaggcgac cggctctcgg cgagttcact ggaccgccac ttcccccatg gacgacggat
      421 caccttccgt tcaacaggcc ggggactgct gcgcgtgggc accggcaaac ccgtgatcga
      481 acgcgactat ccgggcagca tcacgatcag taccgccaac ggtgccctgc tcgtggtgaa
      541 cgatgtcgca ctggacgatt acgtggccgg ggtggtgcag agcgaagcgg gcaaccacaa
      601 aggcgttgag tattacaagt tgcaggccgt cagttgccgc acctatgcga taacgaccgc
      661 gcggcgccac atcaacgagg gtttcaacct ttgcgaccgc acgcattgcc aggtgtacaa
      721 gggcgcctgt cggctggaca gcatcaagaa agccgtgcgc gccacgaaga acctcgtggt
      781 ggtggacgcg gacatccggc ccatccacgc caccttccac agcaactgcg gcggggagac
      841 caccaacgcg gaggatgtct ggagcaagcc cgaatcctac ctggtgagcg cgctggacac
      901 cttctgcctg catgagcccc atgcccaatg gcgcaccgac atgccccgga ccgcatggct
      961 cacctacctc aaggacagct tcgggctgga caccatgaac gccgcgaccg tggaggcggt
     1021 cacttcgttc gacccgcgat gccgatccct gtacctcgat ggtgcgcacc cgtccatttc
     1081 attgttgaag gtgcgtgccg acctcggccc cgccttc
//
LOCUS       DAOQOC010001209         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_127609_length_1116_cov_4.575872, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001209 DAOQOC010000000
VERSION     DAOQOC010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127609_length_1116_cov_4.575872"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /gene="sufC"
                     /locus_tag="PL106_15595"
     CDS             <1..255
                     /gene="sufC"
                     /locus_tag="PL106_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096285.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNA34552.1"
                     /translation="KLGILDETDSGLDIDALRIVAGGVNKLRNADNAFIVVTHYQRLL
                     DHIVPDFVHVMADGRIIKSGPKELALELEERGYDWVKEHA"
     gene            255..>1116
                     /locus_tag="PL106_15600"
     CDS             255..>1116
                     /locus_tag="PL106_15600"
                     /inference="COORDINATES: protein motif:HMM:NF040245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNA34553.1"
                     /translation="MSTATISDPKANLLPLLEGSSWPGSAEALAQAGVLPIPTSRTEA
                     WKYTRVGRLFDQPFAKANGHATVVLPDRLPFAATRVVFVNGHFRADLSDELKVQRGLV
                     IDSLMRHLSHGPVQENYGKLAPTDDRLFTAMNTAAPTDGMILLVTRGVKVERPIHLIH
                     LTTAERQLVQPRDLFMLHDGADAEVIIEHIAVDAPGALINSVRECVVGKGARLTTHKL
                     QREGHRTTAGGPVSINFDGVRVGSRGHFSSSTTTLDGALVRNDVNVVLAGPGAHAELN
                     GVYLLNGTAHC"
BASE COUNT          225 a          336 c          366 g          189 t
ORIGIN      
        1 aagctcggca tcctggacga gaccgacagc ggcctggaca tcgacgcgct gcgcatcgtg
       61 gccggcggcg tgaacaagct gcgcaacgcc gacaacgcgt tcatcgtggt aacgcactac
      121 caacgattgc tggaccacat cgtgccggac ttcgtgcatg tgatggccga tggacgcatc
      181 atcaaaagcg gtccgaagga actggcgttg gaactggaag aacggggcta cgactgggtg
      241 aaggagcacg cgtgatgagc actgctacca tttccgatcc gaaggcgaac ctgctgcccc
      301 tgcttgaagg ctcgtcgtgg cccggatcgg ccgaagcact ggcgcaagct ggcgtactgc
      361 ccattccgac cagcaggacc gaggcgtgga agtacacccg cgtgggaaga ctgttcgatc
      421 agccgtttgc gaaggcgaat ggccatgcga cggtggtgct tccggaccgg cttccattcg
      481 cggccacgcg cgtggtcttc gtgaacggcc acttccgggc cgacctcagc gatgaactga
      541 aggtccagcg tggcctggtg atcgacagct tgatgcgcca cctctcgcat ggtcctgtgc
      601 aggagaacta cggcaagctg gcccccactg acgatcgcct cttcacggcc atgaacaccg
      661 ccgcacccac ggatggcatg atcctgctgg tgacccgggg tgtgaaagtg gagaggccca
      721 tccacctcat ccacctcacc actgcggagc gccagctggt gcaaccccgt gacctgttca
      781 tgctccatga tggtgcggat gccgaggtga tcatcgaaca catcgcggtg gatgcgccgg
      841 gagcactcat caacagcgta cgcgagtgcg tggtgggcaa gggcgcacga ctgacgacac
      901 ataagcttca gcgcgaggga caccgcacga cagcgggagg tcctgtgagc atcaactttg
      961 acggggtgcg cgtgggcagc agggggcact tcagttcgag caccacgacg cttgatggcg
     1021 cgttggtccg gaacgatgtg aacgtggtgc tggccgggcc cggagcgcac gccgaactga
     1081 acggtgtata cctgttgaac ggtaccgcgc attgcg
//
LOCUS       DAOQOC010001210         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_127796_length_1116_cov_1.820924, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001210 DAOQOC010000000
VERSION     DAOQOC010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127796_length_1116_cov_1.820924"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PL106_15605"
     CDS             <1..670
                     /locus_tag="PL106_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNA34554.1"
                     /translation="AAVDEGLLELKPNTSWNVLEAMLQEAPKEIRQQIADNLHWIRSA
                     QQNKLVVGSQARILYADAEGRIRIAEAFNQAIKAGAISAPIVLGRDHHDVSGTDSPYR
                     ETSNIRDGSRFTADMAVQNFVGDAFRGATWISLHNGGGVGWGEVINGGFGMVLDGSTD
                     SDRRLKAMLHWDVNNGIARRAWARNPNAVWSIEQEMKRTPGLQVTLPNEAEEGLIERL
                     VGEV"
     gene            900..>1116
                     /locus_tag="PL106_15610"
     CDS             900..>1116
                     /locus_tag="PL106_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNA34555.1"
                     /translation="MTTAHEKTAIRATWFSIIGNTGLAAIKGLAGSFGNSYALIADAI
                     ESTTDILSSYLVLFGLKYANRPADKNHP"
BASE COUNT          246 a          351 c          362 g          157 t
ORIGIN      
        1 cgcagcggtg gacgaaggtc tcctggagct caagcccaac accagctgga atgtgctgga
       61 ggccatgctg caggaggcgc cgaaggagat ccgccagcag atcgccgaca acctgcactg
      121 gatccgcagc gcgcagcaga acaagctggt ggtgggcagc caggcgcgca tcctctatgc
      181 cgatgccgaa ggccgcatcc gcatcgccga agccttcaac caggccatca aggcgggcgc
      241 catcagcgcg cccatcgtgc tggggcgcga ccaccacgac gtgagcggca ccgacagccc
      301 ataccgagaa acaagtaaca tccgcgatgg cagccgcttt accgccgaca tggccgtgca
      361 gaacttcgtg ggcgacgcct tccgtggtgc gacgtggatc agcttgcaca acggcggcgg
      421 cgtgggctgg ggcgaagtga tcaacggcgg cttcggcatg gtgctcgacg gcagcaccga
      481 cagcgaccgc cgcctgaagg ccatgctgca ctgggacgtg aacaacggca tcgcccgccg
      541 cgcctgggcc cggaacccga acgccgtgtg gagcatcgag caggagatga agcgcacgcc
      601 ggggctacag gtgaccttgc cgaatgaggc ggaggagggg ttgatcgagc gactcgtggg
      661 tgaggtgtga tccaccggtc atcctcgccc gatgtccaga accgccatcc ctatggaacg
      721 ccaggatcgg atcttttcga gcttcgcaag cttcgtggcg accacgaaca acgaaccttg
      781 tacctgctcc acttcccgta ggacaagacc ctgttcatga tcccggtgga accgggaggc
      841 acgcaggggg cccgaaccgt gcaggaagga gcagccggat gaaaagggca gagaggacca
      901 tgacaacagc acacgagaag accgcgatca gggccacctg gttcagcatc ataggcaaca
      961 cgggactggc ggcgatcaaa ggcctcgcgg gatccttcgg gaactcctac gcgctgatcg
     1021 cggacgcgat cgaatcgacc acggacatac tttcctccta cctggtattg ttcgggctga
     1081 agtacgcgaa ccgaccggcg gacaagaacc acccgt
//
LOCUS       DAOQOC010001211         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_127948_length_1115_cov_2.526415, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001211 DAOQOC010000000
VERSION     DAOQOC010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127948_length_1115_cov_2.526415"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1112)
                     /locus_tag="PL106_15615"
     CDS             complement(78..1112)
                     /locus_tag="PL106_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNA34556.1"
                     /translation="MNTVRAPRRGRWMKFATMLALMAVVYALVLREDAGAWGFYGHKR
                     INRMACFTLPPAMFGFYKRHIDFISDHAPDPDRRRYAVPEEAARHYIDIDHYVKPGED
                     PFSVVPRTWDMAVAKFGEDTLQAYGIVPWHIPVVYGRLVRAFQRGDVDRILYYSAELG
                     HYVGDAHVPLHTTENYNGQLTNQHGIHAFWESRIPELSAELNYDHLVGRATYIDDPLG
                     IAWDAVRASHAEVDSVLGIERALNERFPEDLKYVFEDRGRGVMRFQSQEFTRAYEDAM
                     GDMVERRMNASIITLGCFWYSAWVDAGQPDLDRYEQKDVSDSLKMILAAEDEVIKMAK
                     QSLGRPEPNE"
BASE COUNT          199 a          361 c          329 g          226 t
ORIGIN      
        1 aactgcgctt gtgaggcgct gcatcaggta tttacgagcc agggcgatgc caaagcgggg
       61 cgatgcgagc atcgccctta ctcgttcggc tccggtcttc caaggctctg cttcgccatc
      121 ttgatcacct catcttccgc tgcaaggatc atcttcaggc tgtcgctcac gtccttctgc
      181 tcgtaccggt ccagatcggg ctgtccggca tccacccagg ccgagtacca gaaacagccc
      241 agggtgatga tgctggcgtt catccgccgc tctaccatgt cccccatggc gtcctcatac
      301 gccctggtga actcctgaga ctggaaacgc atgacgcccc tcccccggtc ctcaaaaacg
      361 tacttgaggt cttcagggaa gcgttcgttc agggcacgtt cgatccccag cacactgtcc
      421 acctcagcat ggctggcccg taccgcgtcc cacgcgatcc ccaggggatc gtcgatatag
      481 gtggcacgac ccacgaggtg gtcgtagttg agttcggcgc tcaattcggg gatccggctc
      541 tcccagaacg cgtggatacc atgctggttg gtgagttggc cgttgtaatt ctccgtggtg
      601 tgcaggggca cgtgcgcatc gcccacataa tggcccagtt cggcgctgta gtacaggatc
      661 cggtccacat cgccccgctg gaaggcgcgc accagcctgc cgtacaccac ggggatgtgc
      721 cagggcacga tgccgtaagc ttgcaaggtg tcctcgccga acttggccac cgccatgtcc
      781 caggtgcgtg gtaccacgga aaagggatcc tctccgggtt ttacataatg gtcgatgtcg
      841 atgtaatggc gcgcggcctc ctcgggaacg gcgtaccgtc ggcggtcggg gtcgggggcg
      901 tgatcgctga tgaagtcgat gtggcgcttg tagaacccga acatcgcggg cggcagcgtg
      961 aagcacgcca tccggttgat gcgcttgtgc ccgtagaaac cccaggcacc ggcatcctca
     1021 cgcagcacca gcgcatacac caccgccatc agcgcgagca tggtcgcgaa cttcatccac
     1081 cggccccgtc gtggtgctct taccgtgttc atcgc
//
LOCUS       DAOQOC010001212         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_128345_length_1113_cov_3.782609, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001212 DAOQOC010000000
VERSION     DAOQOC010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128345_length_1113_cov_3.782609"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..>1113
                     /locus_tag="PL106_15620"
     CDS             156..>1113
                     /locus_tag="PL106_15620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA34557.1"
                     /translation="MRVAVVHDWLSGPGGAEKVTRELVRLFHADIFALVDLLNDADRA
                     WILGGQRARTTFIQNLPFARAHFRWYLPLFPKAIGSLDLSGYDLILSSSYAVAKGVRK
                     RPGQLHVCYIHTPMRYAWVDQDGYLREHGMHSWKAWIIRRVMRRMRAWDTANSAHVDR
                     FIANSANVRERVRRIYGREADVVLPPVDPNVFTLYNGPREGYLAVSRLVPYKRIDRIV
                     AAFRDLPEQRLTIVGAGPEAQRLREMATPNVTFTGFLPQARLVERMQRARALICTANE
                     DLGLTVLEAQACGTPVIALRQGGYLETVNEEHGGLLFSSDSPT"
BASE COUNT          203 a          369 c          341 g          200 t
ORIGIN      
        1 tacacccgca gcatgatgcg cgccatggtc accgtgaacc aaccgcacat gcgtgctgaa
       61 caagtggccc gaggccatga acgcgcccgt gccttctctg aagaacgcac catggccgcc
      121 ctgcgtgagg tgctggggcc cttgatccag gggtgatgcg ggtggccgtt gtgcacgatt
      181 ggctgagcgg acccggtggt gccgagaagg tgacgcgcga gctggtccga ctcttccatg
      241 ccgatatctt cgctctggtg gacctgttga acgatgcgga ccgagcgtgg atcctcgggg
      301 gccaacgggc acgcaccacc ttcattcaga accttccctt cgccagagcc cacttccgct
      361 ggtacctgcc cttgttcccc aaggcgatcg ggtccctcga cctgagcgga tacgatctga
      421 tcctcagttc aagctatgcc gttgccaaag gagtgcgcaa gcgacccggc cagctccatg
      481 tctgctacat ccatacgccc atgcgttatg cctgggtgga tcaggacggt tatctgcgcg
      541 agcatggcat gcactcctgg aaagcatgga tcattcgccg ggtgatgcgc cgcatgcgcg
      601 cctgggacac cgccaacagt gcgcatgtgg accgcttcat cgccaacagc gccaacgtgc
      661 gcgagcgcgt acggcgcatc tacggtcgcg aggccgatgt ggtgctcccg cccgtggacc
      721 cgaacgtgtt cacgttgtac aacggcccgc gtgagggtta cctcgcggtt tcacggctgg
      781 taccgtacaa gcgcatcgat cgcatcgtcg cggccttccg cgacctgccg gaacaacggc
      841 tcaccatcgt gggtgcaggg cccgaagcgc aacgcctgcg cgaaatggcc acgcccaacg
      901 tgaccttcac cgggttccta ccgcaagctc ggctcgtgga acgcatgcaa cgtgcccgcg
      961 cattgatctg caccgccaac gaggacctcg gcctcaccgt gctggaagcc caggcctgtg
     1021 gcacgcccgt gatcgccctg cgccagggag gatacctgga aacggtgaat gaggaacacg
     1081 gtggacttct attctcctcg gacagcccga cgg
//
LOCUS       DAOQOC010001213         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_128553_length_1113_cov_1.535917, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001213 DAOQOC010000000
VERSION     DAOQOC010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128553_length_1113_cov_1.535917"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PL106_15625"
     CDS             complement(<1..307)
                     /locus_tag="PL106_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNA34558.1"
                     /translation="MSPSLLDFLRHIEHECAYLVQLTEGRDRYALLEDPTNSRAVVRS
                     LEVIGEAVKKLPAELRAKYPQVEWADIAGMRDVLIHNYFGIDYDIVWGVLRKEIPELH
                     "
     gene            complement(288..602)
                     /locus_tag="PL106_15630"
     CDS             complement(288..602)
                     /locus_tag="PL106_15630"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNA34559.1"
                     /translation="MASVNTKRELLRLLHENTVGIRAFGVMRLGLFGSFARDAAGPAS
                     DVDLLVEFAPEQKTLKNLVGLARHLEDLLGRKVELVTPASLNRFIGKHIVQEVEYVAL
                     AA"
     gene            complement(788..>1113)
                     /locus_tag="PL106_15635"
     CDS             complement(788..>1113)
                     /locus_tag="PL106_15635"
                     /inference="COORDINATES: protein motif:HMM:NF037830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34560.1"
                     /translation="VSIFSIREQFGLGAADEEWIPKVGKENGLAITHDINITRTKHQR
                     ELCEKHGVGLIIIRPPSKKHGLPYWEQVQLLVKYWLDILKVATRKSGHYHYELKPKSG
                     LKEMG"
BASE COUNT          210 a          361 c          300 g          242 t
ORIGIN      
        1 ggtgcagttc cgggatctcc ttccgcagca cgccccatac gatatcgtag tcgatcccga
       61 agtagttgtg gatgagcaca tcgcgcatgc ccgcgatgtc ggcccattcc acctggggat
      121 atttcgcgcg caactcggca ggcagcttct tcaccgcctc gccgatcacc tccaggctgc
      181 gcacgacggc ccggctgttc gtggggtcct ccaacagagc gtaacggtcg cggccctctg
      241 tcaactgcac gagataagcg cactcgtgct cgatgtgccg cagaaagtca agcagcgagg
      301 gcgacatact ccacctcttg tacgatgtgc ttgccgatga agcggttcaa cgacgcgggg
      361 gtgaccaact ccaccttgcg gccgagcagg tcctccaaat gacgtgcgag acctaccagg
      421 ttcttcagtg tcttttgctc aggtgcgaat tccaccagca ggtccacatc gctcgcgggt
      481 ccggcggcat cgcgggcgaa ggagccgaac aggcccaacc gcatcacgcc gaaggcgcgg
      541 atcccgaccg tgttctcgtg taagagccgc agcagttcac gtttggtgtt taccgatgcc
      601 atgccacgaa tgtacgcagc aagacttcag ggctcactgg cccggtctac tcctcttccg
      661 atcccgactc atcccccatc ctgtactatg tcgtagttga ccacaaagtc cggctcctct
      721 tccgttaggc cgtagtgcgc cgccagcaac gcgtcgatct cgtcaaggag gggcttgcgc
      781 aatggtatta ccccatctcc ttcaaccccg acttgggctt cagttcatag tggtaatgcc
      841 cgctcttgcg tgttgcgacc ttcaagatgt ccagccagta tttcaccaac agttgcacct
      901 gttcccagta ggggagccca tgcttcttgg atggtggccg gataatgatc aggcccacgc
      961 catgcttctc gcacaattcc cgctggtgct tggtccgcgt aatgttgatg tcgtgcgtaa
     1021 tggccaagcc gttctccttg cccactttgg ggatccactc ctcatccgcc gcacccaggc
     1081 cgaactgttc cctgatcgag aagatcgaaa cgg
//
LOCUS       DAOQOC010001214         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_128702_length_1112_cov_2.222327, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001214 DAOQOC010000000
VERSION     DAOQOC010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128702_length_1112_cov_2.222327"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PL106_15640"
     CDS             complement(<1..775)
                     /locus_tag="PL106_15640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA34561.1"
                     /translation="MPRVLRIINRFNLGGPTHNAAYLTRYLPGDFETLLVGGGQEASE
                     EGSHHILDSLGVQATILPEMQREVAPWRDRGAYRRIKRLIRDFKPDIVHTHAAKAGAV
                     GRMAASELGVKAIVHTFHGHVFHSYFGTVRTAMYKNIERFLARRSSRIIAISEKQKHE
                     LVGEHRITTEDKVSVIPLGFDLSRFQQDQATKRALFRKVYGVADDEIAVGIVGRLVPV
                     KNHDLFLAALKQVRDRSGRKVRAFVVGDGEERERLEALTR"
     gene            complement(913..>1112)
                     /locus_tag="PL106_15645"
     CDS             complement(913..>1112)
                     /locus_tag="PL106_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34562.1"
                     /translation="EAQRIMKRHFNVERIDLLNHTVFPFNQLLSGPSLALARRLSRRV
                     ENAAWLRWLSSDLLIRAVERA"
BASE COUNT          192 a          379 c          326 g          215 t
ORIGIN      
        1 ggcgggtgag tgcttccagc cgctcccgct cttccccgtc gcccaccacg aaggcccgca
       61 ccttgcgacc cgagcgatcg cgcacctgct tcaatgccgc caggaacagg tcatggttct
      121 tcacgggtac caagcggccc acgataccca ccgcgatctc gtcgtccgcc acaccgtaca
      181 ccttgcggaa gagcgcgcgt ttggtggcct ggtcctgctg gaatcggctg agatcgaaac
      241 ccagcgggat cacactcacc ttgtcctcgg tggtgatcct gtgctcgccc accagttcgt
      301 gcttctgctt ctcgctgatg gcgatgatgc ggctgcttcg ccgggccagg aagcgctcga
      361 tgttcttgta catcgccgtg cgcaccgtac cgaagtagct gtggaagacg tgcccgtgga
      421 aggtgtgtac gatcgctttc acccccagtt cgctcgccgc catccggccc acggcccccg
      481 ccttcgcggc atgcgtatgt acgatgtccg gcttgaagtc cctgatcagc cgcttgatcc
      541 gccggtaagc tccccgatcg cgccaaggcg ccacctcccg ctgcatctcg ggcagaatgg
      601 tggcctgcac gcccaacgaa tcgaggatgt ggtggctgcc ctcctcgctc gcctcctggc
      661 caccacctac caacaaggtc tcgaaatcac ccggcaggta gcgtgtcaaa taggccgcat
      721 tgtgcgtggg gccacccagg ttgaaccgat tgatgatgcg aaggacgcgg ggcatggaag
      781 ttcggggcga atgtatcggg atgccacttc gcgtggcggc ctctcctcag cagttgtaca
      841 cgctggaccg tagacgcaca ggctgcgttc ggttgccttc ggaggcctga acagcagtat
      901 cgctgtggcc gctcaggcgc gctccaccgc cctgatcagg agatcgctgc tcaaccagcg
      961 caaccacgcc gcgttctcga ccctgcggga caaccgccgg gccagcgcca gggacggccc
     1021 actcaacaat tggttgaatg ggaagaccgt gtggttcaac agatcgatcc gctccacgtt
     1081 gaaatgccgc ttcatgatcc gttgcgcttc ac
//
LOCUS       DAOQOC010001215         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_128867_length_1111_cov_2.591856, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001215 DAOQOC010000000
VERSION     DAOQOC010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128867_length_1111_cov_2.591856"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PL106_15650"
     CDS             <1..266
                     /locus_tag="PL106_15650"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753995.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNA34563.1"
                     /translation="RQADILVVAAGRPEMITADMVKDGAVVVDVGIHRVPDTSRKSGF
                     RLIGDVKFDEVAPKCAWITPVPGGVGPMTITSLLLNTLKAAQR"
     gene            288..>1111
                     /locus_tag="PL106_15655"
     CDS             288..>1111
                     /locus_tag="PL106_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNA34564.1"
                     /translation="MDPRRIRIADITYELPPERIAQNPLADRDAAKLLVYRDGRITDD
                     RFGSLAAHLPAGSLLVLNDTRVVKARIIFHRRTGARIECMILDPEDHGPMEAALHAIG
                     RSRWWCMVGNAARWDPGETLWTGEGPGALRASRSGTKDGTHLVEFAWDDGAPFADKLE
                     IFGHVPLPPYMKRPDDADDPIRYNTVFAEHDGSVAAPTASLHFTPVLLRSLTARGMGT
                     AHVTLHVGAGTFLPVKSGTMDGHHMHTEQVRIPRATVEMLRDRTGRSPIVPVGTTAL"
BASE COUNT          191 a          362 c          375 g          183 t
ORIGIN      
        1 cccgccaggc cgacatcctg gtggtggccg ctggccgtcc ggaaatgatc accgccgaca
       61 tggtgaagga cggcgccgtg gtggtggacg tggggatcca ccgggtgccc gatacctcgc
      121 gcaagagcgg cttccgcctg atcggcgacg tgaagttcga cgaggtggcc ccgaaatgcg
      181 catggatcac gcctgtaccc ggtggtgtgg gtcccatgac gatcacctcc ctgctgctga
      241 acacgctgaa ggccgcgcag cgctgaggag cgcccttttc gcagccgatg gacccacgcc
      301 ggatccgcat cgcggacatc acgtacgaac tgccgcccga gcgtatcgcc cagaatccgc
      361 ttgccgatcg ggatgcggcg aagttgctgg tgtaccgtga tggccgcatc accgacgatc
      421 gtttcggatc attggccgcg cacctgccgg cgggcagctt gttggtgctg aacgacacgc
      481 gggtggtgaa ggcacgtatc atcttccacc gcaggaccgg tgcgcgcatc gagtgcatga
      541 tactggaccc ggaggatcat gggccgatgg aagcggcatt gcatgcgatc ggcaggtcgc
      601 gttggtggtg catggtgggc aacgctgcgc gctgggaccc tggggagacc ctctggacgg
      661 gggaggggcc cggcgcactc cgggcatcgc gaagcggtac gaaagacggc acccacctgg
      721 tggagttcgc ctgggacgat ggtgcgccct tcgccgacaa gcttgagatc ttcgggcacg
      781 taccgctgcc gccgtacatg aagcgccccg acgatgccga cgatcccatc cgttacaata
      841 ccgtgttcgc ggaacacgac gggtcggtgg ccgcacctac ggcgagcctg cacttcacgc
      901 cagtcctgct gcgttcgctc accgccaggg ggatgggcac cgcacacgtc accctgcatg
      961 tgggcgcggg cacgttcctg cccgtgaagt ccgggaccat ggacggccac cacatgcaca
     1021 ccgagcaggt ccgcattccc cgtgcaacgg tagaaatgct gcgcgatcgc acgggccgct
     1081 ctcccattgt acccgtgggc accacggcgc t
//
LOCUS       DAOQOC010001216         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_129313_length_1109_cov_2.905123, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001216 DAOQOC010000000
VERSION     DAOQOC010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129313_length_1109_cov_2.905123"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PL106_15660"
     CDS             <1..290
                     /locus_tag="PL106_15660"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNA34565.1"
                     /translation="ACLPYTQPKGSSDDFDARVEQFADRTTTIDVRHLEMPLPMLTIL
                     DALEHLPEGHALYVDHKRIPVFLLPELEERGFEYRVKEIADGDVKLFIFKP"
     gene            291..>1109
                     /locus_tag="PL106_15665"
     CDS             291..>1109
                     /locus_tag="PL106_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34566.1"
                     /translation="MTGSPLGGNVARTTSHKVVIPFYAYAAASFLAATVLLLLSADAF
                     TGHWFQPHILAITHLMALGWATMIILGAGHQLVPVLIENDLYSNTLAHASFLLTGTGI
                     PLLVYGFHVFDLGWPAQVGGSLVLGGLLAFISNIAISIAKSKRENVHAVFVFTATIWL
                     LLTVSLGLALLFNFTSSLLPRDSLHYLSLHAHLGVVGWFLLLVLGVGSRLIPMFLISK
                     YNEPRVLWAVFVLVNTALVLYGLFFLFGFAPVLNFIPAALVLAGLLLFVNYCRKA"
BASE COUNT          162 a          382 c          321 g          244 t
ORIGIN      
        1 aagcctgcct gccgtatacc cagccgaagg gatcaagtga tgactttgac gcccgggtgg
       61 aacagttcgc ggaccgcacc accaccattg atgtgcgcca cctggagatg cccctgccca
      121 tgctcaccat cctggacgcg ctggagcacc tgcccgaggg ccatgccctg tacgtggacc
      181 acaagcggat ccccgtgttc ctgctgcccg aactggagga gcgcgggttc gagtaccgcg
      241 tgaaggagat cgccgacggc gacgtgaagc tcttcatctt caaaccctga atgaccggat
      301 caccactggg cggcaacgtg gcgcgcacca cttcccacaa ggtggtgatc ccgttctacg
      361 cgtatgcggc cgcatccttc ctggcggcca ccgtcctgct cctgctctcg gccgatgcgt
      421 tcaccgggca ctggttccag ccgcacatcc tggcgatcac gcacctgatg gccctgggtt
      481 gggccactat gatcatcctc ggtgccggcc accagctggt gccggtgctc atcgagaacg
      541 acctgtacag caacacgctg gcccacgcct ccttcctcct caccggcacc gggatcccct
      601 tgctcgtgta tggtttccat gtgttcgacc tgggctggcc cgcgcaggtt ggcggatcgc
      661 tggtgctggg tggcctgctg gccttcattt ccaacatcgc catcagcatc gccaagagca
      721 agcgcgagaa cgtgcatgcc gtcttcgtct tcacggccac catctggctg ctgctcaccg
      781 tgtcgctcgg gttggcgctg ctgttcaact tcacctcttc cctgctcccg cgcgattcgc
      841 tccactacct gagcttgcac gcccatttgg gcgtggtggg ctggttcctg ctgctggtgc
      901 tgggcgtggg ctcgcggctc atccccatgt tcctcatttc caagtacaat gaaccacgtg
      961 tgctttgggc ggtgttcgtc ctggtgaaca cggcgctcgt gctgtacggg ctgttcttcc
     1021 tgttcggctt cgcgccggtg ctcaacttca ttcccgccgc gctggtgctg gccgggctgc
     1081 tgctcttcgt gaactactgc cgcaaggcc
//
LOCUS       DAOQOC010001217         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_130053_length_1106_cov_2.866794, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001217 DAOQOC010000000
VERSION     DAOQOC010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130053_length_1106_cov_2.866794"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PL106_15670"
     CDS             complement(<1..207)
                     /locus_tag="PL106_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNA34567.1"
                     /translation="MSERILILDHGSQYTQLIARRVRELNVYCEIHPWDKAATFAADP
                     SIKGVILSGSPFSVHDANAPDTDIR"
     gene            402..>1106
                     /locus_tag="PL106_15675"
     CDS             402..>1106
                     /locus_tag="PL106_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34568.1"
                     /translation="MARYISMLALLGLAYSSSGQLVVWAEEFENGCTQNCPATGYAGP
                     NGNWTAMPTGANGACANIWFVSCQENGNAVGACGSGCGTNETLHIGNNAAGCTSPNSC
                     FFCPSGDCGAAYDASCPPGLCSFCCSCQSSQTATRAVSPVIDLTGLSAITLRFKYMEG
                     GAGSSDNALLDYFDGTAWSQLVDLPKTPTAGCGGQGIWTAYSIALPASANNNPGVRIG
                     FRWVNNDDGSGTDPSVA"
BASE COUNT          218 a          326 c          350 g          212 t
ORIGIN      
        1 tctgatatcg gtatcgggtg cgttggcatc atgcacgctg aacggcgagc cgctgaggat
       61 cacccctttg atggacggat cggccgcgaa cgtggcggct ttgtcccatg gatggatctc
      121 gcagtaaacg ttcaactccc gcacgcgccg cgcgataagc tgcgtgtact gcgagccgtg
      181 atcgaggatg aggatgcgct cggacaaggg gatgtgcgat gtggaaagtg tgcgaaagta
      241 tcccggttcg gtgatcgact gaacgacggc cgtggttcgg ccaccgacga ggatcgaccg
      301 ttcccgatcc atcctctctg gaaccaccgg agatagagcg tgcggttgaa aacgccctgt
      361 tgaaccaacc ggaaaattgc tcaacttcgt ggtgatccac catggcacgc tacatctcca
      421 tgctcgcgct gcttggcttg gcgtactcct ccagtggaca actggtggta tgggccgagg
      481 agttcgagaa tggatgcacc cagaactgtc cggccaccgg gtatgccgga cccaatggga
      541 actggactgc catgcctacc ggcgccaatg gagcgtgcgc caacatctgg ttcgtgagtt
      601 gccaggagaa cgggaacgct gtgggggctt gcggtagcgg ttgcggtacc aacgagacct
      661 tgcacatcgg taacaatgcc gctggatgca cctcgcccaa cagttgcttc ttctgcccct
      721 ccggcgattg tggtgcggcc tacgacgcca gttgcccgcc cggactctgt tccttctgtt
      781 gcagttgcca gagcagccag accgccacac gggcggtgtc accggtgatc gacctcaccg
      841 ggctctccgc gatcacgttg cgattcaaat acatggaagg cggagcgggg tcgagtgaca
      901 acgcgctact ggactatttc gatggtacag cctggagcca actggtggac ctgcccaaga
      961 caccgaccgc cgggtgcggt ggacaaggca tctggaccgc gtacagcatc gccctgccgg
     1021 cctcggcgaa caacaacccg ggcgttcgta tcggtttccg ctgggtgaac aacgacgacg
     1081 gtagcggcac ggatcccagc gtggca
//
LOCUS       DAOQOC010001218         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_130130_length_1106_cov_2.129401, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001218 DAOQOC010000000
VERSION     DAOQOC010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130130_length_1106_cov_2.129401"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1106)
                     /locus_tag="PL106_15680"
     CDS             complement(<1..>1106)
                     /locus_tag="PL106_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34569.1"
                     /translation="TTYDEFAIGFSLEPEKVKDRSVDLELPRAFWAMPRVTVQWVAGT
                     GAYDQTFEVNILFLGQTASDRTADTATGTHSDMAIAAQRCLERFIDLYVNGDTLFMGE
                     VVNIERIGAGTLSPIIDEDTTQLTGVNLRIDVTVRASECAAEYFDGIPSETADCDAAT
                     VTNSDDSYMHTVASGATLELPDVTHTDSDGSPVTLPGMEPFVATPCGPGDDAHVLINQ
                     KPVIDLGPGETWDLHVEYPDGTQVGSWNAGAERWEIPACPACDDATAVVKNTAGTTLD
                     TEAIPSGGTEDIIIGDVVITIEDTASTVINVANAPSGEPYTVVAPDAKYQLKDSAGVD
                     IGSPGLIVSNGNANITAPDATYQLKDSAGTNIGGP"
BASE COUNT          176 a          391 c          304 g          235 t
ORIGIN      
        1 ggcccaccga tgttcgtccc ggcgctgtcc ttcagttggt acgtcgcatc tggggcggtg
       61 atgttcgcat tgccgttgct gacgatcaga cccggcgatc cgatatccac ccccgcgctg
      121 tccttcagtt ggtatttcgc atccggcgcg accaccgtgt acggctcacc gctcggggcg
      181 ttcgccacgt tgatcacggt agatgccgtg tcctctatcg tgatcaccac gtccccgatg
      241 atgatgtcct ccgtgccacc gctcggtatg gcctcggtat ccaatgtcgt tccggcggta
      301 tttttcacca ccgccgtcgc atcgtcacag gcggggcatg ccgggatctc ccaccgctca
      361 gccccggcgt tccacgaccc cacctgcgtg ccgtcagggt actccacatg cagatcccac
      421 gtctcccccg gcccgaggtc gatcaccggc ttctgattga tcagcacgtg cgcatcatcg
      481 ccgggtccgc acggcgtggc cacgaacggc tccatccccg gaagtgtcac cggtgatccg
      541 tcgctgtccg tgtgcgtaac atcaggcagt tccagcgttg cgcccgacgc tacggtgtgc
      601 atgtagctgt cgtcgctgtt ggtgacggtg gccgcatcgc agtccgccgt ttcgctcgga
      661 atgccgtcga agtattccgc cgcacattcc gaagcccgca ccgtgacatc tatccgaagg
      721 ttcacccccg tgagttgcgt ggtatcctca tcgatgatgg gcgaaagcgt acccgcaccg
      781 atcctttcga tgttcaccac ctctcccatg aacagggtat ccccgttcac gtaaaggtcg
      841 atgaaccgtt caaggcaccg ctgcgcagcg atcgccatgt cgctatgcgt tcccgttgcc
      901 gtgtccgccg tccggtcgct ggccgtctgc ccaaggaaca ggatgttaac ctcaaaggtc
      961 tgatcatacg cccccgtgcc cgccacccac tgcaccgtca cgcgcggcat ggcccagaac
     1021 gcgcgcggca actccaggtc cacgctgcgg tccttcacct tctccggctc caagctgaag
     1081 ccgatggcga actcatcata ggtggt
//
LOCUS       DAOQOC010001219         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_130273_length_1105_cov_3.372381, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001219 DAOQOC010000000
VERSION     DAOQOC010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130273_length_1105_cov_3.372381"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PL106_15685"
     CDS             complement(<1..623)
                     /locus_tag="PL106_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA34570.1"
                     /translation="MAHLPPLIADLGLILSVAAVTTIISKVVKQPVVLGYIVAGLLVG
                     PHVNLVPSVMDEKNLVTWSEIGVIFLLFSLGLEFSFRKLMKVGGTAVVTTMVTVSFMM
                     GAGFLTGRALGWAMMDSLFLGGILSMSSTTIIIRAFDELGLKTASFAKVVLGVLVVED
                     LVAILLLVLLSTVAVSREFSGGALLFEMGKLGFFLVLWFVVGIFLIPS"
     gene            787..>1105
                     /locus_tag="PL106_15690"
     CDS             787..>1105
                     /locus_tag="PL106_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34571.1"
                     /translation="MRSFFLCAAYALSLFCGAQTLVPDTLTPAIVESVRPDIARLPAV
                     QDTWIMSGRKTEAIDVDARDAALTEKYARQIFAKVPGIFVYDMDGTGNQLNIAARGLD
                     PHRS"
BASE COUNT          246 a          364 c          315 g          180 t
ORIGIN      
        1 gatgggatca aaaaaatgcc caccacgaac cacaggacca ggaagaagcc cagcttgccc
       61 atctcgaaga gcaatgcgcc accggagaac tcccggctca ccgccaccgt gctcagcagc
      121 accagcagca ggatggccac caggtcctcc accaccagca cgcccagcac caccttggcg
      181 aaggaggcgg tcttcaaacc cagctcatca aaagcccgga tgatgatggt ggtggaactc
      241 atcgagagga tccccccgag gaagaggctg tccatcatgg cccagcccag ggcccggccg
      301 gtaaggaacc ccgcacccat cataaaagat actgttacca tggtggtcac cacggccgtg
      361 ccgcccacct tcatcaattt gcggaaactg aactcaaggc ccaggctgaa cagcaggaag
      421 atcacaccga tctcgctcca ggtgaccagg ttcttctcgt ccatgacgga cggcaccagg
      481 ttcacatgcg gccccaccag caggcccgcc acgatgtagc ccagtaccac gggttgtttc
      541 accaccttgg atatgatggt ggtgaccgcg gccaccgaaa gaatgagccc caggtcggcg
      601 atgagcggag ggaggtgcgc catgcgtcgg tcgttgaacc gtgtttgcgg caaggtaggc
      661 atcgcgtatc ggatgtcccg atccgttgtg tcacacgcat cgtacaccgt gatccgcatg
      721 cccgatggca cggtccgcac aacggatcgg ctcccgaagg gccaacggat agtttggctc
      781 gccaccatgc gatccttctt tctgtgcgcg gcgtacgcgc tatccctctt ttgtggcgcg
      841 caaacgctcg tacccgatac gctcaccccg gccatcgtgg aaagtgtgcg cccggacatc
      901 gcacggctgc ccgccgtgca ggacacttgg atcatgagcg ggcgcaagac cgaagcgatc
      961 gacgtggacg cgcgcgatgc cgcactcacg gagaaatatg cgcgacagat cttcgccaag
     1021 gtgcccggca ttttcgtgta cgacatggat ggcaccggca atcagctcaa catcgccgcg
     1081 cggggcctgg atccacaccg tagct
//
LOCUS       DAOQOC010001220         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_130727_length_1103_cov_3.183206, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001220 DAOQOC010000000
VERSION     DAOQOC010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130727_length_1103_cov_3.183206"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1103
                     /locus_tag="PL106_15695"
     CDS             <1..>1103
                     /locus_tag="PL106_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34572.1"
                     /translation="LDAFDGEEETVRATLVALSQQVEREAMDDERHTVFDRLLVASAG
                     SPLRWLVNAIDRATGDPHLRHVLWEGCRPGIVITPHRSPLSRTFCQGPDQPIYYFQNG
                     TRGVNGGPLVILGELEPDLVLGTEQRGELLTAARGVLIGHQRETDPVTCCEHRAIAHH
                     RLDQGIGISLLPLPPGRRTALDAYVGYVAYVNRVPVAYGGAWLFPGRTKVGINVFPAF
                     RGGPSALLFARILRCYAQRYAVDAFEAENYQLGHGNGDGIRSGAYWFYHRLGFRSQHP
                     RLAAIAAREAERMRADPGYRTPARVLRKLAAEPMLLRLREKDVPHVEPLDVAERALHY
                     LAKVTKGDRHAARERIALRVARRLGAGSMKRWS"
BASE COUNT          188 a          334 c          373 g          208 t
ORIGIN      
        1 gctcgatgcg ttcgacgggg aggaggagac cgtgcgcgct accctggtcg cactttcgca
       61 acaggtggaa cgcgaggcga tggatgatga acgccacacc gtgttcgacc gcttgctcgt
      121 ggcctcggcc ggctcaccgt tgaggtggtt ggtgaatgcc atcgaccgtg cgaccgggga
      181 tccgcatctg cgccacgtgc tctgggaggg atgccgaccg ggtatcgtca tcacccccca
      241 tcggtcgccg ctctcacgca cgttctgtca gggaccggac caaccgatat attacttcca
      301 gaacggtacg cgtggcgtga acggaggacc attggtgatc ctcggggagc tcgaaccgga
      361 cctggttctc ggaacggagc aacggggtga actgctcacc gccgcacgtg gtgtcctcat
      421 cgggcaccag cgcgagaccg atccggtcac ctgctgcgag cacagggcga tcgcgcacca
      481 tcggctcgat caggggatcg gcatctccct gctcccgttg ccacccggcc gccgtacggc
      541 gctcgatgcg tacgtgggct atgtggcgta tgtgaacagg gtgccggtgg cctatggcgg
      601 agcctggttg ttcccgggac ggaccaaagt gggtatcaat gtcttccccg ctttccgtgg
      661 cggaccgtca gccctgctgt tcgcacggat cctgcgctgc tacgcacagc ggtatgctgt
      721 ggatgctttt gaggcggaga actatcagct gggacatggc aacggcgatg gtatccgctc
      781 aggggcctat tggttctacc atcgtctggg tttccgcagc cagcatccac gacttgccgc
      841 gatcgcagcg agagaagcgg aacggatgcg tgcggatccg gggtatcgaa ctccggcccg
      901 ggtgttgcgc aaactcgccg ccgagccgat gctgttgcgg ctccgcgaga aggacgtgcc
      961 gcatgttgaa ccattggacg ttgcggaacg cgcgctgcat taccttgcca aggtgacgaa
     1021 aggagaccgg cacgccgcgc gcgaacggat cgcattgcgg gtcgcgcgca gactcggtgc
     1081 gggttccatg aagcgttggt ccg
//
LOCUS       DAOQOC010001221         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_131298_length_1101_cov_2.208413, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001221 DAOQOC010000000
VERSION     DAOQOC010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131298_length_1101_cov_2.208413"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PL106_15700"
     CDS             complement(<1..926)
                     /locus_tag="PL106_15700"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNA34573.1"
                     /translation="MKRQHPDMSVQELCRSLGVSRQAHYRQAHRSERVLQGLTEVVEL
                     VRGIRATQPKLGGGKLYTILAPRIAALPVAFGRDRFFELLSREGLTLRKRKRRVRTTM
                     SRHGLPVYHDLVKGRQVNAPGQVWVADITYWPVREGFYFIFLITDACSRKIIGHQVAR
                     GMSGEHALAALRMAQNNSPHDLNGIIHHSDRGKQYCFGQYVRQLMQAGMQISMTESSD
                     PRDNAVAERVNGTLKNELLAHHNVHDIDQARQLLDDAVHIYNTQRPHLSCDLQYPEQA
                     HRLDHGLNRRWKHRIYKGTVNLLQDEPGVVNSP"
BASE COUNT          191 a          343 c          311 g          256 t
ORIGIN      
        1 ggtgagttta caacgcccgg ttcatcctga agcaggttga cagtaccctt atagatgcgg
       61 tgtttccaac ggcggttcag gccatggtcc aaccggtgtg cctgttccgg gtactgcaga
      121 tcacaactca ggtgcggccg ttgggtattg tagatgtgaa cggcatcatc caggagctgt
      181 cgtgcctggt cgatgtcatg cacgttgtgg tgggctagca gctcgttctt cagggtgccg
      241 ttcacacgtt cggctacggc attgtccctg ggatccgagg actcggtcat gctgatctgc
      301 atgcccgcct gcatgagctg acgcacgtac tgcccaaagc aatactgctt gcctcggtca
      361 ctgtgatgga tgatgccgtt caagtcgtgc ggactgttat tctgcgccat gcgcagcgca
      421 gccagtgcgt gttcaccgct cataccccgg gccacttgat ggccaatgat cttgcgtgag
      481 cacgcatcgg tgatcaggaa gatgaagtag aacccctctc gcacaggcca gtaggtgatg
      541 tcggccaccc atacctgtcc aggcgcgttc acctgccggc ctttgaccag gtcatggtac
      601 acgggtaatc cgtgacggct catggtggtg cgaacgcgcc ttttgcgctt gcgcagcgtc
      661 agtccttcgc ggctgagcaa ctcaaagaag cggtccctgc cgaacgctac gggaagcgct
      721 gctatccgcg gggccagtat cgtgtacaac ttgccacctc ccagcttggg ttgggtggcc
      781 cttatgccac gcaccagttc cacgacctcc gtcagtccct gcagaacacg ctcactgcga
      841 tgagcctgac ggtagtgtgc ctgcctgctc acaccaaggg atcgacacag ctcctgcacg
      901 ctcatatccg gatgctgtct cttcatctcc cggatcactt ggtgccggac ttttttcgga
      961 tcgccacctt cagctccttc tcggccaggt cgatcatcat ggaatacgcc tcggccttca
     1021 gctcagcggc gcgcaggtcc ttgcgcaatc gttccacttc ctggcgcagg cgttcggcct
     1081 cttccgcctc gccttcaacg g
//
LOCUS       DAOQOC010001222         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_131433_length_1100_cov_3.332057, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001222 DAOQOC010000000
VERSION     DAOQOC010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131433_length_1100_cov_3.332057"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PL106_15705"
     CDS             <1..517
                     /locus_tag="PL106_15705"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA34574.1"
                     /translation="ICSGLSVATTNESSGPAGLNYSWDMGDGTVLSGPDATHLYAGQG
                     SYTITLTAVDTVCLGSDMYTFEVTVTPGAPPAELFVVPNVFSPNGDGKNETFFPLPEV
                     SGSVVSLKVFNRWGQKLYETGNTFRPWNGKADLTDPVPAGVYYYVLDYRVPCSGQVLE
                     GERKGLVQLLR"
     gene            556..>1100
                     /locus_tag="PL106_15710"
     CDS             556..>1100
                     /locus_tag="PL106_15710"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA34575.1"
                     /translation="MLLLLWSLCGQVVAQADTSVRVDTTTWMDSTRHRLIPIALYHLP
                     TDTVHVGPLAIISHGWNRNLPDTYLKYSYLAERLAAHGYLVVSIQHELPGDEPLAMGG
                     DLFALRAPNRERGAATIAFVIDAVKHRWPTVGQDRTILIGHSNGGDMSVLFAERHPEA
                     ITELITLDNRRVPLPRMSRPR"
BASE COUNT          213 a          350 c          334 g          203 t
ORIGIN      
        1 catctgttcg gggctgagtg tggccaccac caacgagagt tcagggccgg ccggtctcaa
       61 ttacagctgg gacatgggcg atggcaccgt gctctccggc ccggacgcca cccacctcta
      121 cgctggccag ggcagctata ccatcacgct gacggccgtg gataccgtat gcctcggcag
      181 cgacatgtac accttcgagg tgacggtgac cccgggcgca ccacccgccg aactcttcgt
      241 cgtgcccaac gtgttcagtc ccaacggcga tgggaagaac gagaccttct ttccccttcc
      301 tgaagtgagc gggtccgtgg tgtccttgaa agtcttcaac cggtggggcc agaaactgta
      361 cgaaacaggg aacacgttca gaccgtggaa cggcaaagcg gacctcaccg atcccgtgcc
      421 ggcaggtgtg tactactacg tactggacta tcgcgtaccc tgttcgggcc aagtgctcga
      481 aggcgagcgg aagggtctgg tccaattgct gcggtgagca cgcgcatgcg atcgccatcc
      541 gggtgctctt ggatcgtgct tctgctgctc tggtccctct gcgggcaagt ggtcgcacaa
      601 gcggatacgt ccgtacgcgt ggataccacc acgtggatgg acagcacgcg ccatcgcctg
      661 atccccatcg cgctctatca cctgcccacc gacaccgtac acgtcggtcc actggccatc
      721 atcagccacg gctggaacag gaacctgccc gacacctatc tgaaatacag ctatctcgcc
      781 gagcggctcg cggcgcatgg atacctcgtg gtaagtatcc aacacgaatt gcctggcgat
      841 gaaccgttgg ccatgggggg tgatctgttc gccttgcgcg cgccgaaccg ggaacgggga
      901 gccgcgacca tcgccttcgt gatcgatgcg gtgaagcacc gctggcccac ggtgggccag
      961 gatcggacca tcctgatcgg ccattccaac ggtggggaca tgagcgtgtt gttcgcggag
     1021 cggcaccctg aagcgataac cgaattgatc accttggaca atcgtcgggt gccactgccc
     1081 cggatgagcc gaccgcggat
//
LOCUS       DAOQOC010001223         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_131728_length_1099_cov_2.709770, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001223 DAOQOC010000000
VERSION     DAOQOC010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131728_length_1099_cov_2.709770"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PL106_15715"
     CDS             complement(<1..423)
                     /locus_tag="PL106_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA34576.1"
                     /translation="MIKIAVTGPESSGKTTLVEALAHHYGCVYTQEYARVYLEEYGPD
                     YMEEDLVRIASGQLMVEAEAQELATDKGTPLILCDTDMLTVRIWSEEKFGRAADLLKK
                     LTRRVRYQHWLLCRPDIPWAPDPLRENPHDRDRLFTVYE"
     gene            686..>1099
                     /locus_tag="PL106_15720"
     CDS             686..>1099
                     /locus_tag="PL106_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34577.1"
                     /translation="MERSLLSISAITIAIATNAQLAPTTEARLTDHLLEVNAQWRVMD
                     AAPAGGERVVHFTNEAERIATHLHMVAAHARTRTPEGLSAAALAERAVLLEKLDAYAD
                     RGIFPQNHVLPYRNPIFIDPHGTACAVGQLMVESGN"
BASE COUNT          204 a          391 c          298 g          206 t
ORIGIN      
        1 ctcgtacacc gtgaagagcc ggtcgcgatc gtgcgggttc tcgcgcaaag gatccggcgc
       61 ccacggtatg tcaggccggc acagcaacca gtgctggtaa cgcaccctgc gcgtcagctt
      121 cttcaacagg tccgccgcac ggccgaactt ctcctcgctc cagatccgca ctgtgagcat
      181 gtccgtatcg cacaggatca gtggggtccc tttatctgtc gccagttctt gcgcttcggc
      241 ttccaccatc agctgcccgc tcgcgatccg caccaggtcc tcttccatat agtccggtcc
      301 atactcctcc aggtacacac gtgcatactc ctgtgtgtac acgcatccgt agtggtgcgc
      361 cagcgcctcc accaacgtgg tcttgccgct gctctccggg cccgtaacgg cgatcttgat
      421 cacgcccgaa aggtagcgac gtcaacgctc tgacaccccc gtacacgacc cggcacgatc
      481 gatcccgctc agcacattga gggtgtgacc ttcattcttc cgcgcacccc ggtattccac
      541 ggtcggtgga gaagaagccg ttgctccttg gttggagtgg atgagggtca aagcggtgtg
      601 cttggtcacc gatccattcg ttcatcccac ctcaggcagc gcgggaggga tcccctgcgt
      661 ctactccatg aaaacgacct tacacatgga acgaagcctc ctctccatct ccgcgatcac
      721 gatcgcgatc gccacgaacg cacaactcgc ccccaccacg gaggcgcgcc tgaccgatca
      781 cctgctggaa gtgaacgccc agtggcgcgt gatggacgct gcacccgccg gcggggaacg
      841 cgtcgttcac ttcacgaacg aggcggaacg catcgccacg catctgcaca tggtggccgc
      901 ccatgcacgc acccgcaccc ccgagggcct ttcggccgca gccctcgccg aacgggccgt
      961 gctgctggag aaactcgatg cctatgcgga tcgcgggatc ttcccgcaga accatgtgct
     1021 gccctaccgc aaccccatct tcatcgatcc acacggcacc gcatgcgccg ttggccagct
     1081 gatggtggaa agcggtaac
//
LOCUS       DAOQOC010001224         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_132182_length_1097_cov_3.615163, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001224 DAOQOC010000000
VERSION     DAOQOC010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132182_length_1097_cov_3.615163"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PL106_15725"
     CDS             complement(<1..504)
                     /locus_tag="PL106_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNA34578.1"
                     /translation="MKLTRVPRSGMAMLVAMLCPGLLRAQDTTDRYDEVLERIDALSA
                     LPWLKNDPFTTDVRALNKHGFAPTDTPKWAPEVLRQRLADLDARTPFSLVWNAPVESY
                     IAMYTVRKREQAARMLGLAQLYFPVFEEALDRYNLPQELKYLAVVESALYPGARSRAA
                     AVGLWQFM"
     gene            complement(806..>1097)
                     /locus_tag="PL106_15730"
     CDS             complement(806..>1097)
                     /locus_tag="PL106_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34579.1"
                     /translation="TGRRIRLGDELLVTIRGVDMDKRTVDLGIAEGRTAPEDPRGYTG
                     NNTFAAVARPSDTRGQWDETHPSNADERAGRKRAQAQARERQRRSLGRGKRR"
BASE COUNT          192 a          340 c          351 g          214 t
ORIGIN      
        1 catgaactgc cagagtccca cggcggcggc acgcgagcgg gcgcccggat acagcgcgct
       61 ttccaccacg gccagatatt tcaattcctg tggcaggttg taccggtcca gggcctcttc
      121 gaaaacgggg aagtacaact gggccagacc gagcatacgt gcggcttgct cccgcttgcg
      181 cacggtgtac atggcgatgt acgattccac cggcgcgttc cacacgaggc tgaacggtgt
      241 gcgcgcgtcc agatcggcca ggcgttggcg cagcacttca ggggcccatt tcggcgtgtc
      301 cgtgggcgcg aatccatgtt tgttgagtgc gcggacgtcc gttgtaaaag ggtcattctt
      361 cagccacgga agggcgctca gggcatcgat ccgttccagc acttcgtcgt accggtccgt
      421 ggtgtcctgg gcgcggagaa gacctgggca cagcatagct acaagcatgg ccatgcccga
      481 ccggggaacg cgggtcagtt tcatgaggac gctaaaagta gccttcttac gcacgccgga
      541 ccgcatggtt ctaaggcatg atgcacgatc aggtgtgaac gggaccatcg gtgaaaagca
      601 cgatcgcccc gacgggaaca cattccggcg cgatgtggga tcaggtggag aacgaccggg
      661 aggtccggat cggtacctca tcgacgggct tttcgagggc tgaggatggc aggtcttgct
      721 ctccgtacga cctgatcatg cggtggatcg cgaggagcaa ctttccgcga tgcggacgtt
      781 ccgggtagcg ggagagcacg ccacgctacc tccgttttcc gcgccccaag cttcttcgtt
      841 gccgctctcg ggcttgtgcc tgtgcccgct tccggccggc ccgttcatcg gcgttcgaag
      901 gatgtgtctc gtcccattgt ccacgcgtat ccgaaggcct ggcgacagcc gcgaacgtgt
      961 tgttgcccgt gtacccgcgc ggatcctccg gggccgtacg cccttcggcg atcccgagat
     1021 ccacggtgcg cttgtccatg tccaccccac ggatcgtcac cagcagttca tcgccaagtc
     1081 ggatgcgccg cccggta
//
LOCUS       DAOQOC010001225         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_132191_length_1097_cov_3.411708, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001225 DAOQOC010000000
VERSION     DAOQOC010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132191_length_1097_cov_3.411708"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..899)
                     /locus_tag="PL106_15735"
     CDS             complement(318..899)
                     /locus_tag="PL106_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNA34580.1"
                     /translation="MLGLKLATDPRWAELVDQDIGAILSDHAWCEQKAASNAISLIVR
                     YPEHLDMVKELTRIAQEEITHFGMVVERIEARGLILEHERKDHYVNQLLTFVRKDGSR
                     EERLVDRLLFSAMIEARSCERFKMLSERISDPDLRAFYRDLMVSEAGHYTTFIGFARQ
                     FAPGLDVEARWQAFLAFEAELIASYGKKPTMHG"
     gene            complement(905..>1097)
                     /locus_tag="PL106_15740"
     CDS             complement(905..>1097)
                     /locus_tag="PL106_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34581.1"
                     /translation="EVGAFWNVQLIDALGRTVHTTVWPIGLRQLVVPVGDLPAGSYVV
                     RCQALDGYGNATTPLVIAR"
BASE COUNT          203 a          343 c          339 g          212 t
ORIGIN      
        1 atggcaacgg ggtagtcttt cggcaggctc agcgcgacac cactttgggg atctttatga
       61 actgtcacgc tgagcgtgcc gaagcgctat acaccacaga acggtccaga cgcgacccgc
      121 tgaaaacagt gcggcaattg cttggtggtg cttcgaggtg ctcagcgcga cagcacctgg
      181 gagatcgaac cgcattggaa tagcgcttcg gtaggctcag cgtgacattg cagcggagcg
      241 ttcatggtac cggggtagcg attcgatcct tccttcctca ggacacgctc actcagcgtg
      301 atcgtgcgcc ctccctccta cccgtgcatc gtcggcttct taccgtagct ggcgatcaat
      361 tccgcttcga atgccaggaa ggcctgccag cgtgcttcca catcgagccc cggtgcgaac
      421 tggcgcgcga agccgatgaa cgtggtgtag tgcccggcct cggagaccat cagatcgcgg
      481 tagaatgcac gcaggtcggg atcgctgatg cgctcgctga gcatcttgaa ccgctcgcag
      541 ctgcgcgcct cgatcatggc gctgaaaagc aagcggtcca ccaagcgctc ctcgcgcgag
      601 ccgtccttgc gcacgaaggt gagcagttgg ttcacgtagt ggtccttgcg ctcgtgttcc
      661 agtatcaggc cacgcgcctc gatgcgttcc accaccatgc cgaagtgggt gatctcctcc
      721 tgcgcgatcc gcgtgagttc cttcaccatg tcgaggtgct ccggataacg cacgatgagc
      781 gagatggcgt tgctggcggc cttctgctcg caccacgcat ggtcgctgag gatggccccg
      841 atgtcctggt ccaccagttc ggcccagcgc gggtcggtgg cgagtttgag gccgagcatg
      901 ccggtcagcg ggcgatcacc agcggtgtgg tggcgtttcc gtaaccatcc agcgcttggc
      961 aacgcaccac gtacgaaccg gcgggcaggt cgcctaccgg gaccacgagt tgccgcaggc
     1021 caatgggcca aaccgtcgta tggacggtgc ggcccaacgc atcgatcagt tggacattcc
     1081 aaaaagcacc gacctcc
//
LOCUS       DAOQOC010001226         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_133026_length_1094_cov_2.794995, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001226 DAOQOC010000000
VERSION     DAOQOC010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133026_length_1094_cov_2.794995"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PL106_15745"
     CDS             complement(<1..330)
                     /locus_tag="PL106_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34582.1"
                     /translation="MSIGSTDSGMSARSIGALLLRYWYLFLIGPALAIAWAYWHLRVT
                     VPMYEVKAQMLIKEEKSYGEDLLFAELGIGKKVKNLENEVLVMLSSPLMADVVRQKNM
                     QWRYFRQD"
     gene            complement(327..>1094)
                     /locus_tag="PL106_15750"
     CDS             complement(327..>1094)
                     /locus_tag="PL106_15750"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNA34583.1"
                     /translation="WLPLLLPLLFSCGSVKLKQMVMFDDVRSEPLTIDTLPQLRIAPL
                     DIINIQVTSNDPSTVLAFQQQRLTMANSMNAGSGESALGVQEGYRVDEQGEIHMPFIG
                     AVRAQGRTLNELRQEISTRLDKYILNTSVQCRFMNFRVTLLGEVNRPSTYTVPSERVT
                     ILEALGMAGDFTAYAERNNVLVIRERDGVRSTARVNVQDREIFRSPWFYLNPGDIVYV
                     RPQKARQYATRGDLVERYGVLLVSLLSVAATVIITTR"
BASE COUNT          200 a          356 c          328 g          210 t
ORIGIN      
        1 atcctgccgg aagtagcgcc actgcatgtt cttctggcga accacgtcgg ccatcaaggg
       61 cgaggacaac atcaccagca cctcgttctc cagattcttc accttcttgc cgatccccag
      121 ttcggcgaag agcaggtcct ccccgtagct cttctcctcc ttgatgagca tctgggcctt
      181 cacctcgtac atgggcacgg tgacacgcag atgccagtag gcccacgcga tcgccaatgc
      241 gggaccgatg aggaacagat accagtaacg caatagaagt gcgccgatcg atcgcgcact
      301 catcccgctg tccgttgaac cgatgctcat cgcgtggtga tgatcacggt ggccgccacg
      361 ctcagcagcg agaccagcag caccccgtac cgctccacca ggtcgccccg ggtggcgtat
      421 tgccgggcct tctgtggccg cacgtacacg atgtcgccgg gattcaggta gaaccagggc
      481 gaccggaaga tctcccggtc ctgcacgttc acccgggcgg tggagcgcac gccatcccgc
      541 tcgcggatca ccagtacgtt gttccgctcg gcgtaggccg tgaaatcgcc ggccatgccc
      601 agggcctcca ggatggtgac gcgttcgctg ggcacggtat aggtgctcgg ccggttcacc
      661 tcacccagca aggtgaccct gaagttcatg aagcggcatt gcaccgaggt gttcaaaata
      721 tacttgtcca gccgggtgct gatctcctgc cgcagctcgt tcagcgtgcg gccctgggcc
      781 cgtaccgccc cgatgaacgg catgtggatc tctccctgct cgtccacacg gtacccttcc
      841 tgcaccccca aggcgctctc gcccgagccc gcgttcatgc tgttggccat ggtgaggcgt
      901 tgctgctgga aggcgagcac cgtgctgggg tcgttgctgg tgacctggat gttgatgatg
      961 tcgagcggcg cgatccgtaa ctggggcagt gtatcgatgg tcaggggctc ggaacgcaca
     1021 tcgtcgaaca tcaccatctg cttcagcttc acgctgccac agctgaaaag cagggggagc
     1081 aggaggggca gcca
//
LOCUS       DAOQOC010001227         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_133775_length_1091_cov_3.126448, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001227 DAOQOC010000000
VERSION     DAOQOC010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133775_length_1091_cov_3.126448"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PL106_15755"
     CDS             complement(<1..1036)
                     /locus_tag="PL106_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34584.1"
                     /translation="MRTLLPTLLLTLIIQATHAQSIAGNLHEGTGRHALGYGNVDIFR
                     GDALIASVLTDRYGNFNVRLDTGYYKCVVHYDGYESETRMVRVKADEKVDFAVIEAKD
                     GSKRRPASGSAVTRSSSDASERATYASPRRELKYVSTTAASTVVSADAPSPSDGESTR
                     IPLADGGVIEKKAGHGVLTAGEVNDFAKWTQWQDIAGNTLSALKDLWAMAPSGRYTLD
                     LQNKSGLPIADAVVHLKKKDGTELYSARTDNTGKAELWAALDPMAPLPKERLFMEVEY
                     RGRTTRVDNVKPFERAVNRMVLDVPCGPSDLVDVAFVVDATGSMQDELDFLTAEMNDI
                     IFRSKRINDKL"
BASE COUNT          177 a          338 c          333 g          243 t
ORIGIN      
        1 tcagcttgtc gttgatgcgt ttggagcgga agatgatgtc gttcatctcg gccgtgagga
       61 agtcgagttc gtcctgcatg gagccggtgg catccaccac gaaagccaca tccaccaggt
      121 cgctggggcc gcacggcacg tccagcacca tccgattcac tgcgcgctca aaaggtttca
      181 cgttgtcgac gcgggtggtt cgaccccggt attcgacctc catgaacaag cgctccttcg
      241 gaagtggggc catcgggtcc aatgcagccc agagttccgc cttgcccgtg ttgtcggtac
      301 gcgcgctgta cagctcggtg ccatcctttt tcttcagatg aaccacggca tccgcgatcg
      361 gcagcccgct cttgttctgc aggtccaggg tgtaacgacc gctgggtgcc atggcccaca
      421 ggtctttcaa tgcgctcagc gtattcccgg cgatgtcctg ccactgcgtc cacttcgcga
      481 agtcgttcac ttcacccgct gtgagcaccc cgtgtccggc tttcttttcg atcacccccc
      541 catcggccaa cggtatccga gtggactcac cgtccgatgg agagggtgcg tcggcgctga
      601 ccacggtgga agcggccgtt gtcgatacgt acttcagctc gcgtcgtggt gacgcatagg
      661 tcgcacgttc gctcgcatcg gacgaggacc tggtgacagc cgaaccggat gctggtctgc
      721 gctttgatcc gtccttggcc tcgatcaccg cgaagtcgac cttttcatcg gccttcaccc
      781 gcaccatccg ggtctcgctc tcgtacccgt cgtagtggac cacacacttg tagtaccccg
      841 tgtccagccg cacgttgaag ttcccgtacc ggtccgtgag caccgaggcg atcagtgcgt
      901 cgccacggaa gatgtccacg ttgccatatc cgagggcgtg gcgtccggtg ccttcgtgca
      961 ggttgcctgc gatggattgc gcgtgtgtgg cctgaatgat cagcgtcagc aggagggtgg
     1021 gaagcagtgt tcgcatgagg gtgggttcgg gtgtgatgtg tctgcataca cgccggaccc
     1081 gaggaggttc g
//
LOCUS       DAOQOC010001228         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_133920_length_1091_cov_1.879344, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001228 DAOQOC010000000
VERSION     DAOQOC010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133920_length_1091_cov_1.879344"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /gene="dnaX"
                     /locus_tag="PL106_15760"
     CDS             complement(<1..>1091)
                     /gene="dnaX"
                     /locus_tag="PL106_15760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNA34585.1"
                     /translation="EHVTGTLKNAIRNNHLASAFLFTGPRGVGKTTCARILARTINCE
                     SLGTDLVPCGKCAPCVTFDEGHSLNIFELDAASNNSVDDIRNLIQQVQIAPQVGSKKV
                     YIIDEVHMLSPAAFNAFLKTLEEPPSYAIFILATTEKHKILPTILSRCQVFDFRRITV
                     TDIAKQLGTIAEKEGIQVEEQALHTIAQKADGGMRDALSIFDQLVSFAGERLTYQDVV
                     KNLNVLDHEHYFAITDHLLRNDTAAALVAYNEVLFQGFDGHLFVAGLARHFRDLLVSQ
                     DARTIPLLEVSSDVAGRYATQAQAVSRDLLVRGLERLGQIDTQYKNSKEPRLLVELVL
                     IQLSAPVGQGAQDRPAASLEKKKPDVSTV"
BASE COUNT          191 a          322 c          342 g          236 t
ORIGIN      
        1 gacggtgctt acgtcaggct tttttttttc caggctggcc gcaggccggt cctgcgcacc
       61 ctggccgacc ggggcggaca actggatcag caccaactcc accaacaagc ggggctcctt
      121 gctgttcttg tattgggtat cgatctgccc caagcgttcg agaccacgca cgagaaggtc
      181 gcgggacacg gcttgcgctt gcgtggcgta ccgaccagcc acatccgaac tcacttccaa
      241 caggggaatg gtccgcgcat cctgtgaaac aagcaggtcg cggaaatgcc tggccaggcc
      301 cgctacgaag aggtgcccgt cgaagccctg gaacagtact tcgttgtagg ccaccagtgc
      361 cgcggccgta tcattcctca gcaggtgatc ggtgatggcg aagtagtgct catggtccag
      421 cacgttcaga tttttcacca cgtcctggta tgtgagccgc tcgcccgcga agctcaccag
      481 ttgatcgaag atgctgagtg cgtcgcgcat gccgccgtcc gccttctgcg caatggtgtg
      541 cagggcctgc tcttcgacct ggatgccctc tttttcggcg atcgtgccca attgcttggc
      601 gatgtccgtc accgtgatgc gcctgaagtc gaacacttgg caacggctca ggatggtggg
      661 caggatcttg tgcttctccg tggtggccag gatgaagatg gcgtaggagg gaggctcctc
      721 cagcgtcttc aggaaggcat tgaaggctgc cgggctcagc atatgcacct cgtcgatgat
      781 gtacaccttc ttgctgccca cctgtggagc gatctgtacc tgctggatca ggttgcggat
      841 gtcgtccacg ctgttgttgc tcgcagcgtc cagctcgaag atgttcaggc tgtggccttc
      901 gtcgaaggtg acgcagggag cgcatttccc gcagggcacc agatcggtac ccaagctctc
      961 gcagttgatc gtgcgcgcca gaatgcgggc gcaggtggtc ttgcccacgc cgcgcggacc
     1021 ggtgaacagg aaggccgagg ccaggtggtt gttccgaatg gcgttcttga gcgtgcccgt
     1081 gacatgctcc t
//
LOCUS       DAOQOC010001229         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_133992_length_1090_cov_4.765217, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001229 DAOQOC010000000
VERSION     DAOQOC010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133992_length_1090_cov_4.765217"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..>1090
                     /locus_tag="PL106_15765"
     CDS             327..>1090
                     /locus_tag="PL106_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34586.1"
                     /translation="MKHTIALVCFVLLCGHVAAQQVQTFNVVRQNLVQGPALGLSVKE
                     VPEGYLVFQVQRGLSGTPQDPFVSLFDRQGELVWEHDYVVPRYGFLGYPDPVCRTPDG
                     GFASGVSVFGWTSDIDSLFLWRFDAQGDTVFTRFLMADSTMTIRKCIRTMNGDFVFTG
                     LHHPPVEQYLLRTDSMGNIKHYAGFANFDGYGGMGLAEDPSGNFYLTGFQDNIAHRGS
                     LIKCDSVGNLLWTRLYEMENGISEWLSALVLSDSSVL"
BASE COUNT          211 a          305 c          331 g          243 t
ORIGIN      
        1 ccgtggaggt gagcgccttg ccagcaggga tgtactactt gcacctgcgc gatgcgaagc
       61 ggtggttggc ggggagcaag ctggtggtgg agtgagagca gccgttcctc aaatgatcct
      121 gccggaagtc ggagacttcc acgagctttg agcatggatg cacagcgcac aagtggcccg
      181 cccgtcagcg cacatagccc cccgcgcgga acacttgcgc gaattctgga acttgacctg
      241 tcccgcttgg ccaatggcgt gtaccaattg accgtgcctg gaacggtcga agaggaacgc
      301 atcatcgtta cgcattgacc atccccatga agcatacgat cgcgctggtc tgctttgtcc
      361 tgctgtgcgg ccacgtggcc gcgcagcagg tgcagacctt caatgtggtg cggcagaacc
      421 tggtccaagg acctgcactg gggctttctg tgaaggaggt gccggaagga tacctggtct
      481 ttcaggtaca acgtggattg tctggaacgc cgcaggaccc ctttgtcagc ctgttcgacc
      541 ggcagggcga acttgtctgg gaacacgatt atgtggtacc ccggtacgga ttcctcggtt
      601 atcccgaccc cgtttgccgc acgcctgatg gcggcttcgc ttcgggcgtg tccgtgttcg
      661 gctggacatc cgatatcgac agcctctttc tctggcgctt cgatgcacag ggcgatacgg
      721 tcttcacacg tttcctgatg gccgatagca ccatgaccat ccgcaaatgc atccggacga
      781 tgaacgggga cttcgtgttc accggtctgc atcatcctcc tgttgagcaa tatctgttgc
      841 gcacggacag catgggcaac atcaagcact acgctggctt cgccaacttt gatggatatg
      901 gaggaatggg gctggccgaa gacccatcag gcaatttcta ccttaccggt tttcaggata
      961 atattgctca ccggggcagt ctgatcaagt gcgacagcgt gggtaacctg ttgtggacgc
     1021 ggctttacga gatggagaac gggatatccg aatggctctc ggccctggtc ttgtccgact
     1081 cgagcgtgct
//
LOCUS       DAOQOC010001230         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134017_length_1090_cov_3.685990, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001230 DAOQOC010000000
VERSION     DAOQOC010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134017_length_1090_cov_3.685990"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..>1090
                     /locus_tag="PL106_15770"
     CDS             57..>1090
                     /locus_tag="PL106_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34587.1"
                     /translation="MDIRDLDYDPVNEQIVVTGYYSAPQQFPIDTLWGGCLEESYFGF
                     VVCFDTEGNTLWAKQITGGQISIMETSIASDGSVHVFGISRYGSATFQESPILSTPQG
                     SFHAVYSSTGMLNSAQHLSGSAVDVYGGAVCADGSLVLCGWIKQAGSILGEPFAPSEG
                     DTAAFVLKSDTSGSNIDWIDVLPSTASASTGKCIILPNGPIIIAGGFQDDVIVQSDTL
                     QSPAGISNGFITAIQLDGEIRWVRQLESTGVGGYASALDYDGADQLYLSTGYTGEMRL
                     GGTIELPSVAQRNGLIARIDTLGNWQAACVMGGTYRGNFVSIGPDGLFFSCGYDSTWT
                     MGTTTVPYPVG"
BASE COUNT          227 a          291 c          318 g          254 t
ORIGIN      
        1 ccggcaacct tctttgggct cgccacatac tgagcacttg ttggtacagc gatctcgtgg
       61 atatacgcga cttggactat gatccggtaa atgagcagat cgtggtgacg ggatattaca
      121 gcgcaccgca acagttcccg atcgacacat tgtggggggg ctgtttggag gagtcgtact
      181 tcggcttcgt ggtctgcttc gatacggagg ggaacactct atgggcgaag cagatcaccg
      241 gaggccagat ctccataatg gagacatcca ttgcgagtga cggatccgtg catgtgttcg
      301 gtatttcgcg atacggaagc gccacattcc aagaatcacc tatcctgagc accccgcaag
      361 gcagtttcca tgcggtgtac tcctccacgg gaatgctgaa tagtgcgcaa cacctgagcg
      421 gctcggccgt tgatgtgtac ggaggcgccg tttgtgcgga cggtagtttg gtgctctgtg
      481 gctggataaa gcaggccggt agcatactcg gcgagccatt cgcacccagc gagggtgata
      541 cagccgcgtt tgttttgaag agcgacacct cgggaagcaa cattgattgg atagatgtac
      601 ttccttcaac agcttcggcc tccactggga agtgcatcat cctgcccaat ggaccgatca
      661 tcattgccgg cggattccaa gacgatgtca ttgtgcagtc cgacacgctt caatcccctg
      721 cgggcatctc caacggattc attaccgcaa ttcaactcga tggcgagatc cgttgggtgc
      781 gacagctgga gtctaccgga gttgggggtt acgcatccgc ccttgattat gatggtgcgg
      841 accaattgta tctttccaca gggtacactg gtgaaatgcg gttgggcggc accattgagc
      901 ttccctcggt cgcccaacgg aatgggttga tcgcacgcat cgacaccctc gggaattggc
      961 aggccgcgtg cgtcatggga ggcacctacc gtggtaactt cgtgtccatt ggcccagatg
     1021 gtctcttctt ctcgtgtgga tatgactcca cttggaccat ggggaccacc accgtaccgt
     1081 atccagtagg
//
LOCUS       DAOQOC010001231         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134019_length_1090_cov_3.639614, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001231 DAOQOC010000000
VERSION     DAOQOC010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134019_length_1090_cov_3.639614"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PL106_15775"
     CDS             complement(<1..812)
                     /locus_tag="PL106_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNA34588.1"
                     /translation="MPEPTTTVLRSETIDTSVLLKAWELMCSAKAMTELYEEHFKFTS
                     KYVHATSRGHEAIQLALGLQLKPQDFVSPYYRDDSILLGIGMRPYELMLQLMAKRDDP
                     FSGGRSYYCHPSLKREGMPRIPHQSSATGMQGIPATGIALGLWYKERAGIPHDMNGGD
                     GGAPVVVCSFGDASITEGEVAEAFQMAVLKSLPIIYLVQDNEWDISASADEFRKGDAT
                     AYAKGFPGLETRTIDGTDFFESYATLNEVIGLVRKERRPFLVHAKVPLLNHHT"
     gene            911..>1090
                     /locus_tag="PL106_15780"
     CDS             911..>1090
                     /locus_tag="PL106_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNA34589.1"
                     /translation="MSRTSHELDHRIVGDDMQCVEITLDPQETVLAEAGAFMMMDEGI
                     EMKTIFGSGNGEEQSF"
BASE COUNT          207 a          324 c          342 g          217 t
ORIGIN      
        1 gtatggtgat tcagcaaagg caccttggca tggacgagga acgggcggcg ctctttgcgc
       61 accaggccga tcacctcatt cagcgtggca tagctctcga agaaatccgt cccatcaatg
      121 gtgcgtgtct ccagtcccgg gaagcctttg gcgtatgcgg tggcgtcgcc cttgcggaac
      181 tcgtcggcac tggcgctgat gtcccattcg ttgtcctgca ccaggtagat gatgggcagg
      241 cttttcagca cggccatttg gaacgcctcc gccacctcac cctcggtgat cgatgcatcg
      301 ccgaaggagc acacgaccac gggagcaccg ccatcgccac cgttcatgtc gtgcggaatg
      361 cccgcgcgct ccttgtacca caggcccaat gcgataccgg tggcggggat cccctgcatg
      421 ccggtggcac tgctctggtg ggggatccgt ggcatgccct cgcgcttgag actcggatgg
      481 cagtaatagc tgcgaccgcc actgaacggg tcgtcgcgct tagccatcaa ctggagcatc
      541 agctcgtacg gacgcatgcc gatgcccagc aggatgctgt cgtcccggta gtagggcgat
      601 acgaagtcct ggggtttcag ctggagtccg agcgcgagtt ggatggcctc gtgcccacgg
      661 ctggtggcat gcacgtactt gctggtgaac ttgaagtgct cctcgtagag ttcggtcatc
      721 gccttggccg agcacatcaa ctcccatgcc ttcagcagca cggaggtatc gatcgtttcg
      781 gacctcagga cggtggtggt cggttctggc atcgctgttc tacaggaacg gccaaattag
      841 ctgttggccc gctgcgcgtc cgaaagacct ccggatacct ttggcccggc aacagagccg
      901 cacaaccacc atgagccgca cttcgcacga attggaccac cgcatcgtag gtgatgacat
      961 gcaatgcgtg gagatcacgc tggacccgca ggagaccgta ctggccgagg ccggggcctt
     1021 catgatgatg gatgagggca tcgagatgaa gaccattttc ggcagtggca acggggagga
     1081 gcagagcttc
//
LOCUS       DAOQOC010001232         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134235_length_1089_cov_4.143133, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001232 DAOQOC010000000
VERSION     DAOQOC010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134235_length_1089_cov_4.143133"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PL106_15785"
     CDS             <1..346
                     /locus_tag="PL106_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34590.1"
                     /translation="KENGTAVFNGLVDDIGIAVDGQVIIGLFENESLPGISLVPNPAN
                     EQVRLNFNAMKGTAVVTITDLAGQVYSSATLNEVYQRSYTMDTRSLAQGAYLVSVVKG
                     TTKYQQRLVVTH"
     gene            501..>1089
                     /locus_tag="PL106_15790"
     CDS             501..>1089
                     /locus_tag="PL106_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34591.1"
                     /translation="MKNLYTAIITGTALLLATAGQAQHTAHGKLAHRLLSVGQHGPQL
                     QGEQRGGGPTNDDCGSVTAENLNIGGSVNFTGSTAGATDVGDYEAGSQLDGEAPSVWH
                     AFTISDCADVTVSYCGTDPVFGNAWIVLATSCPASALVFNNNFDQTTCSDGNLTIRYL
                     GLAAGTYYMPVMLDTAVANGPYTISGDVWSVGMEGS"
BASE COUNT          235 a          314 c          327 g          213 t
ORIGIN      
        1 caaggagaat ggcacggccg tgttcaatgg tctggtggat gatatcggga tcgctgtgga
       61 tggacaggtg atcatcggcc tcttcgagaa cgagagcctg cccggcatca gcttggtgcc
      121 caaccccgcg aacgagcagg tccgcctgaa cttcaacgcg atgaaaggga cagccgttgt
      181 gaccatcacg gacctggccg gccaagtgta ttccagcgca acgctgaatg aggtgtacca
      241 gcgctcctac acgatggaca cccgttcgtt ggcccaaggt gcgtacttgg tctcggtggt
      301 gaaaggaaca acgaagtacc aacagcgcct ggtggtgaca cactgatcac cccgggattg
      361 ccgaaggaga agccgtcctc aggggcggct tcttttttgg ccaccggatc ggaggtctct
      421 tgtcaaaaac tcccggccat tccatttgga ggatcgggtt gttccgtcat ctttggcgtc
      481 ctgacacaac caaccaacac atgaagaacc tgtacacggc gatcatcacc ggaacggccc
      541 tgctgcttgc cacggctggc caagcacaac acaccgcaca tggcaagctt gctcatcggc
      601 tcctctccgt aggacaacat ggccctcagc ttcaaggtga acaacgcggc ggtgggccta
      661 cgaatgacga ttgcggctcg gtgacagcgg agaacctgaa tattgggggc agtgtcaact
      721 tcaccggcag caccgcaggg gccaccgatg taggggacta cgaggccgga tcccagttgg
      781 atggcgaagc accttcggtt tggcatgcat tcacgatcag tgattgtgcc gatgtcacgg
      841 tgtcctattg tggcacggat ccggtcttcg gtaacgcgtg gatcgttctg gccaccagct
      901 gcccggccag cgctctggtc ttcaacaaca atttcgacca gacaacctgt tccgatggca
      961 atctgaccat cagatatctc gggctcgcgg caggcacgta ctacatgccc gtgatgctcg
     1021 atacggccgt agcgaacggg ccgtacacga tcagcggcga cgtgtggagt gtcggcatgg
     1081 agggcagcg
//
LOCUS       DAOQOC010001233         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134266_length_1089_cov_3.301741, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001233 DAOQOC010000000
VERSION     DAOQOC010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134266_length_1089_cov_3.301741"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PL106_15795"
     CDS             <1..660
                     /locus_tag="PL106_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNA34592.1"
                     /translation="SLRLNVAAQNASVLWSDGSTNDSILVVGSATITVTLALDGCLAS
                     DQVNVVFMPVVDDLDLGPDATICLGDELVLDAGTPLATYSWNTGEGGPTITVTRPGIY
                     HVVLTGPCINAADTVAITEGNCAPLVNVPNAFTPDGDGTNEVFAPVVSGTVRAWELRI
                     FDRWGEELFNSNEPHKGWDGTFNGTPVQDGVYVWQLGYKAVSDDGVKQEKLTGSVTLL
                     R"
     gene            complement(663..>1089)
                     /locus_tag="PL106_15800"
     CDS             complement(663..>1089)
                     /locus_tag="PL106_15800"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNA34593.1"
                     /translation="LTLKKKNRWILADHGGRALGKSDHDAVREFRSGFATVRTGDRYG
                     LVDSTGALVLPVKYPFLGDVSKGLLIAADPESGLLGIISTSGTVRVPFAYDAIEFVDD
                     PLALLGRGEGERITDMRLAYMRISDGRYIWKEEGFDAEH"
BASE COUNT          217 a          331 c          347 g          194 t
ORIGIN      
        1 agtctccggc tgaacgtggc ggcccagaac gcgtcggtgc tgtggagcga cggcagcacc
       61 aacgattcca tcctcgttgt cggcagcgcg accatcaccg tcacacttgc actggatggc
      121 tgcctggcca gtgatcaggt gaacgtggta ttcatgcccg tagtggatga tctggacctg
      181 ggaccggacg ccacgatctg ccttggggat gaactggtgc tggatgcggg aacgcctctg
      241 gcgacctatt catggaacac cggcgagggt ggtccgacga tcaccgtgac acgtcccggc
      301 atctaccatg ttgtgctcac cggcccatgc atcaacgccg cggacaccgt cgcgatcacg
      361 gaggggaact gcgccccgtt ggtgaacgtg ccgaacgcgt tcacaccgga tggtgacggc
      421 acgaacgagg tctttgcgcc ggtggtcagc ggaactgtac gcgcatggga gctgaggatc
      481 ttcgatcgct ggggcgagga actgttcaac agcaacgagc cccacaaggg ctgggatggc
      541 acgttcaacg gcacgcccgt gcaggacggg gtgtacgtgt ggcaactggg gtacaaggcg
      601 gtgagcgacg atggggtgaa gcaggagaaa ctcaccggca gcgtgaccct gctgcgttag
      661 gatcagtgtt cagcgtcgaa cccctcctcc ttccagatat atcgaccatc ggatatgcgc
      721 atgtacgcca gccgcatatc cgtgatgcgt tcgccttcgc cacggccgag gagcgccagt
      781 ggatcatcca cgaattcgat cgcgtcataa gcgaacggca cacgaacggt acccgaggtg
      841 ctgatgatcc cgagaagtcc gctttccggg tcggcggcga tgagcagacc cttgctcaca
      901 tcgcccagga acgggtactt gacgggaagt accaaggcac cggtactgtc caccaacccg
      961 tagcgatcgc cggtccggac cgtggcgaac ccgctacgga actcccgcac agcatcgtgg
     1021 tccgacttgc ccagcgcacg tcccccatgg tcggccagga tccagcggtt cttcttcttg
     1081 agagtcagg
//
LOCUS       DAOQOC010001234         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134468_length_1089_cov_1.470019, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001234 DAOQOC010000000
VERSION     DAOQOC010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134468_length_1089_cov_1.470019"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PL106_15805"
     CDS             complement(<1..295)
                     /locus_tag="PL106_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34594.1"
                     /translation="MSKYLLIPLLLGLLAACRKEPVMPPEPQIALVSLDRDSVVEFAD
                     RVVVRFSYQDGDGDLGQPDPDAYTLWVKDARLAQADGYHIPPLAPEGEEVPIQG"
     gene            complement(292..>1089)
                     /locus_tag="PL106_15810"
     CDS             complement(292..>1089)
                     /locus_tag="PL106_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34595.1"
                     /translation="AWLQQRVFGPTCANSGCHDGTFEPDFRSLGSSYSSLVYHPVIAN
                     DPEESFSYRVLPGDVAQSFLYERLTSFVPNTSGIMPVETDGPDWELNEAQYIAAIAAW
                     IQAGAPDAFGRLPGLGNMEPQVTGFQVFPPGNTTTPFPRVDAPGVQPIEVPAGLVDLR
                     FAFSDDSTVASAFTYNKVKVATSVLGFATVPELALTVGAGITAQDFTGAPTTFTHAAT
                     IDLNAYASGTELFVRVYVNDGDHDQPTEVPDDGTGPPMVDHFTLRIQ"
BASE COUNT          186 a          349 c          370 g          184 t
ORIGIN      
        1 cgccctggat gggcacttcc tcgccttcgg gcgctagcgg tggtatgtgg tagccatccg
       61 cttgcgccag tcgcgcatcc tttacccaca gcgtgtaggc atcggggtcc ggctgaccca
      121 ggtcaccgtc accatcctga taactgaagc gcacgaccac gcgatcggcg aactccacca
      181 ccgaatcacg gtccaaggag accagggcga tctgcggttc cggcggcatc accggttcct
      241 tccggcaggc cgccagcagg ccaagcagca aggggatcag caggtacttg ctcattggat
      301 gcgcagggtg aagtggtcga ccatcggcgg accggtcccg tcatccggca cctccgtggg
      361 ctggtcatgg tcgccatcgt tcacatagac ccgcacgaaa agctcggtcc cgctcgcgta
      421 ggcgttcagg tcgatcgtag ccgcatgcgt gaacgtggtg ggcgctccgg tgaagtcctg
      481 cgctgtgatg cccgcgccca ccgtgagggc gagctcgggc accgtggcaa agcccagcac
      541 gctggtggcc accttcacct tgttgtaggt gaaggcactt gctaccgtgg agtcgtccga
      601 gaacgcgaag cgcaggtcca ccaggccggc aggcacttcg atgggctgta cgccgggcgc
      661 atccacccgg gggaagggtg tggtcgtgtt gcccggaggg aatacctgga acccggtgac
      721 ctgcggctcc atgttgccga ggcccggcaa ccggccgaaa gcatcgggcg caccggcctg
      781 gatccaggct gcgatggccg cgatgtactg cgcctcgttc agctcccagt ccggcccgtc
      841 ggtctccacc ggcatgatgc cgctggtgtt gggcacgaag ctggtgagcc gctcgtagag
      901 gaaggactgc gcgacatccc cgggcaacac gcggtagctg aagctctcct ccggatcgtt
      961 ggcgatcacg ggatggtaca cgagcgagct gtaggaggag ccgaggctgc ggaagtccgg
     1021 ttcgaaggtg ccgtcgtggc agccgctgtt cgcacaggtg ggcccgaaca cgcgctgctg
     1081 caaccaggc
//
LOCUS       DAOQOC010001235         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134547_length_1088_cov_3.030010, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001235 DAOQOC010000000
VERSION     DAOQOC010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134547_length_1088_cov_3.030010"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PL106_15815"
     CDS             complement(<1..1082)
                     /locus_tag="PL106_15815"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNA34596.1"
                     /translation="MRVEFVYEPGQYSIRGGIVDVFSYGSDKPYRIELYGDTVESVRR
                     FDPQDQLTVEFLAEAVIVPDLQDEDTARQQTFFAQLPEDTVLWLRDLQAIGDAATKQL
                     KLLGEAYEKLPEREKHPAPNELLATDSELIKGSLGFRKVFWGSSVHHRGTEDTENTST
                     ATAQRRNGNATQDSKAQVAVNFQHRPQPAFAKEFKVLAGDLHNKRNAGYTNLIACNSA
                     KQSERLHAIFTDMEGGDAALGNSSFTPLMLDLHEGFIDPELKLALYTDHQIFERYHRY
                     RLKEGFRKNSQALTLKELTQLKPGDLVVHIDHGIGVFSGLETIDAGGSKQEAIRLKYR
                     DNDILYVGIHSLHRVSKYSGEEGGKAP"
BASE COUNT          155 a          337 c          346 g          250 t
ORIGIN      
        1 ggtgccttgc cgccctcctc gccgctgtac ttgctcacgc ggtgcaggct gtggatgccc
       61 acgtagagga tgtcgttgtc gcggtacttc aggcggatgg cctcctgctt gctgccgccg
      121 gcatcgatgg tctccaggcc gctgaacacc ccgatgccgt ggtcgatgtg caccaccagg
      181 tcgccgggct tcagctgcgt gagctccttc agcgtgagcg cttgcgagtt cttgcggaag
      241 ccctccttca gccggtaacg gtggtagcgc tcgaagatct ggtggtcggt gtagagcgcc
      301 agcttcagct cggggtcgat gaagccctcg tggaggtcga gcattagcgg cgtgaagctg
      361 ctgttgccca acgcggcatc gccgccttcc atgtcggtga agatggcgtg cagccgctcg
      421 ctctgcttgg cgctgttgca ggcgatcagg ttggtgtagc cggcgttgcg cttgttgtgc
      481 aggtcgcccg cgagcacctt gaattccttg gcgaaggcgg gttgtgggcg gtgttggaag
      541 ttcactgcca cctgcgcctt gctgtcttgc gttgcgttcc cgttgcgtcg ttgcgccgtt
      601 gcggttgatg tattctccgt gtcctctgtg cctctgtggt gaaccgagct cccccagaac
      661 accttcctga accccaatga ccctttgatc aactcactgt ccgtggcgag cagttcgttg
      721 ggcgcaggat gcttctcgcg ttccggtagt ttctcgtacg cctcacccaa caacttcaac
      781 tgcttcgtcg ccgcatcgcc gatggcctgg aggtcgcgga gccaaagcac ggtgtcctcg
      841 ggcagttggg cgaagaaggt ctgctggcgc gcggtgtcct catcctgcag gtcgggtacg
      901 atcaccgctt cagcgaggaa ctccacggtc agttggtcct gtggatcgaa gcggcgcacg
      961 ctttccacgg tatccccgta gagctcgatg cggtaaggct tgtcgctgcc gtagctgaac
     1021 acgtccacga taccgccgcg gatgctgtac tgccccggct cgtacacgaa ctccacgcgc
     1081 acgaagcc
//
LOCUS       DAOQOC010001236         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_134744_length_1087_cov_4.255814, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001236 DAOQOC010000000
VERSION     DAOQOC010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134744_length_1087_cov_4.255814"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1087)
                     /locus_tag="PL106_15820"
     CDS             complement(<1..>1087)
                     /locus_tag="PL106_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34597.1"
                     /translation="TWFGDGYATISHTRDGTLWGNVNEVTFRITPHHGVPDGIDTIGT
                     IDFSNAREQFGALTVVEDTVRDKLYGIYENGIVEVDRASGRLEYLIQDSNTLNWLQAL
                     NPVIDRKGMMWLATFRGLYRFDPVRRQLTSIGTSDPDLGPLISTLKWSMFDRTGTLWL
                     GTAGYGVLKYDPRIERFNNQASGSVRALAMTNDGQVLISHYNSYLTVFDPGPGRYSTR
                     IKRVDQRWPAIGAELPTQYAEMTVQDRDGVYWSYLTYGILGRYDPKAGTFELVRPARE
                     PGEPEDGFLFPLHIGTDSALWCGGNQGLWRIDTRRKACVVHPWPTAAVNNPYPFTTAI
                     HQGSDGVVWVGTVKGLLRFDPHADTWRI"
BASE COUNT          201 a          336 c          327 g          223 t
ORIGIN      
        1 gatccgccat gtgtccgcat ggggatcgaa acggagcagg cccttcaccg tgcccaccca
       61 gaccacaccg tccgaacctt gatggatcgc tgtggtgaag gggtacggat tgttcaccgc
      121 tgctgtgggc cagggatgca ccacgcatgc cttcctgcgc gtgtcgatcc gccagaggcc
      181 ttggttgcct ccgcaccaca gcgcgctgtc cgtaccgatg tgcaacggga agaggaaacc
      241 atcctccggt tcgccaggtt cacgcgccgg tctcaccagc tcgaatgtgc ccgccttcgg
      301 gtcataccgg ccgaggatac cgtacgtaag gtaggaccag tacacgccgt cgcgatcctg
      361 cacggtcatc tccgcatact gcgtcggaag ttccgcaccg atcgcgggcc agcgctgatc
      421 aacgcgtttg atccgcgtgc tgtaccgccc gggtccggga tcgaagaccg tcaggtaact
      481 gttgtagtgc gagatgagta cctggccatc attcgtcatg gccagagcgc gtacacttcc
      541 gctcgcctgg ttgttgaagc gttcgatccg gggatcgtac ttgagtacgc cgtagccggc
      601 ggtgcccaac cagagcgtgc ccgtgcggtc gaacattgac cacttcaacg tgctgatcaa
      661 cggtccgagg tccggatcgg atgtgccgat ggacgtgagt tggcgtcgga cgggatcaaa
      721 tcggtagagt ccacggaacg tcgccaacca catcatgccc ttcctgtcga tcaccggatt
      781 gagcgcctgc agccagttca gggtgttgct gtcctggatc agatactcga gtcggccgct
      841 ggcccggtcc acttcaacga taccgttctc atagatgccg tacaatttgt cgcgcacggt
      901 atcctccacc accgtgagcg caccgaactg ttccctggca ttggagaagt cgatggtgcc
      961 gatggtgtcg atcccgtccg gtacaccatg atgaggggtg atccggaacg tgacctcgtt
     1021 gacgttgccc cagagcgtac catcgcgtgt gtggctgatg gtggcatagc catcgccgaa
     1081 ccacgtt
//
LOCUS       DAOQOC010001237         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_135168_length_1086_cov_2.534433, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001237 DAOQOC010000000
VERSION     DAOQOC010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135168_length_1086_cov_2.534433"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PL106_15825"
     CDS             complement(<1..852)
                     /locus_tag="PL106_15825"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNA34598.1"
                     /translation="MSKIKVAKPVVELDGDEMTRIIWKQIKDQLILPYVDVPIEYYDL
                     GIEYRDRTEDKVTVDAANAIKKHNVGIKCATITPDEARVKEFGLKQMWKSPNGTIRNI
                     LNGTVFREPIVIKNIPRLVPGWTQPIVIGRHAFGDQYRATDAVIKGKGKLTLTFQPAD
                     GSPATSWNVYDFEGDGVALSMYNTDESIEGFARSCFNLALQKKWPLYFSSKNTILKKY
                     DGRFKDIFQGIYEKDYAAQFKELGITYEHRLIDDMVASALKWSGGFIWACKNYDGDVQ
                     SDIVAQGF"
BASE COUNT          179 a          316 c          334 g          257 t
ORIGIN      
        1 gaaaccctgc gccacgatgt cgctctgcac gtcgccatcg tagttcttgc aggcccagat
       61 gaagccgccg ctccacttca gggcgctggc caccatgtcg tcgatcagcc ggtgctcgta
      121 ggtgataccg agttccttga attgggccgc gtagtccttc tcgtagatac cctggaagat
      181 gtccttgaat cgcccgtcgt acttcttcag gatggtattc ttgctgctga agtacagggg
      241 ccatttcttc tgcaacgcca ggttgaagca cgagcgcgcg aagccctcga tgctctcgtc
      301 ggtgttgtac atgctcaggg ccacaccgtc gccctcgaag tcatacacat tccaactggt
      361 ggcgggcgat ccatcggcgg gctggaaggt gagtgtgagc ttgcccttgc ccttgatcac
      421 cgcatcggtg gcgcggtact gatcgccgaa ggcatggcgg ccgatgacga tgggctgcgt
      481 ccaacccggt accagccgcg ggatgttctt gatcacgatg ggctcgcgga agacggtccc
      541 gttgaggatg ttgcggatgg tgccgttggg gctcttccac atctgcttca ggccgaattc
      601 cttcacccgt gcctcgtccg gcgtgatggt ggcgcacttg atgcccacgt tgtgcttctt
      661 gatggcgttc gccgcgtcca ccgtcacctt gtcctcggta cggtcgcggt attcgatgcc
      721 gagatcgtag tattcgatgg gcacgtccac gtacgggagg atcagctggt ccttgatctg
      781 tttccagatg atgcgcgtca tctcatcgcc atcgagttcc actacgggct tcgctacctt
      841 gatcttggac atgcgttgaa cgccgtagcc cggcgtaggc gtttagggct tgggttgggt
      901 gggcgaaagt atcgcaaggc ccgtctgttg gcccgaacga tcctgatacc atttgcgcga
      961 tcaaggccga ccaggagccg cgccgtgatc tcccgcttgc gggaaggcca agcggatcgg
     1021 ctggtattga atgcatggat gctacaactt ccggctggaa gtcatcgttg gcccggacct
     1081 ggcttg
//
LOCUS       DAOQOC010001238         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_135340_length_1085_cov_3.685437, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001238 DAOQOC010000000
VERSION     DAOQOC010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135340_length_1085_cov_3.685437"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PL106_15830"
     CDS             complement(<1..374)
                     /locus_tag="PL106_15830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA34599.1"
                     /translation="MLQRTLYSVTLAGTMLACGAAPGTGNEPTASAPKPVTITEQPTE
                     PQKPVQATDNGEGLFAIINTTKGVIKLKLEYQKVPMTVANFVALAQGKQHNTAKPDGT
                     PYFNGIKFHRVIADFMIQGGDPT"
     gene            complement(446..970)
                     /locus_tag="PL106_15835"
     CDS             complement(446..970)
                     /locus_tag="PL106_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34600.1"
                     /translation="MRLIPIAALFTLAAADPCCAQTINITGETVDGRTITLPQVSAGK
                     HCIIAVAASQKAEPMLEAWLEPVYNRLVLKSGLMVSDVDCDLWLVPVFTGMNKVAYGP
                     MMERLKAEADPDIAQRVVFVKDDNTALLDALGMKNLDEPYFFVLGPDGARKHTEHGAY
                     NVDKLDALEEALEK"
BASE COUNT          174 a          298 c          365 g          248 t
ORIGIN      
        1 gtggggtcgc cgccctggat catgaagtcg gcgatcaccc ggtggaattt gatcccgttg
       61 aaatagggcg tgccatcggg cttggccgtg ttgtgctgct tgccctgggc caacgccacg
      121 aagttggcca ccgtcatggg cactttctga tactcgagtt tgagtttgat aacacccttg
      181 gtggtgttga tgatcgcgaa gaggccttcg ccgttgtcgg tggcttgcac gggtttctgg
      241 ggttcggtgg gttgttcggt gatggtaacc ggcttggggg ccgaggcggt gggctcgttg
      301 ccggtgcccg gtgcggcgcc acaggcgagc atggtgcccg caagcgttac ggagtacagg
      361 gtgcgttgca gcatttctgg ggttgttggt cgttgttggg tgttcgggtg gaacagggcg
      421 cgaatgtatc cgctgggccc ctgtatcatt tttccaaggc ttcttcgagc gcgtcgagtt
      481 tgtccacgtt ataggcgccg tgctcggtgt gttttcgtgc cccatcgggg ccgagcacga
      541 agaaataggg ctcgtccagg ttcttcattc cgagcgcatc gagcaacgcg gtattgtcgt
      601 ccttcacgaa caccacgcgc tgggcgatgt cggggtcagc ctcagctttc aaccgctcca
      661 tcatgggccc gtacgccacc ttgttcatac cggtgaacac cggaacgagc cagaggtcgc
      721 agtccacatc gctcaccatg aggccgctct tgagcaccaa gcggttgtac acgggttcca
      781 gccaggcctc cagcatgggc tcggccttct ggctggcggc cacggcaatg atgcagtgct
      841 tgcccgccga gacttgcggc aaggtgatgg tacgcccatc aaccgtttca cccgtgatgt
      901 tgatggtctg cgcgcagcaa ggatcggctg cggccagggt gaacagggcg gcgatgggga
      961 tcaggcgcat gggcgtgatg gaacggatgg gcgccactgc caagtatgat gccacgttcg
     1021 gaaccacccg gatcgagttc aaggttgttg gctcccgagc acgttccggt tcttgcccac
     1081 gtact
//
LOCUS       DAOQOC010001239         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_135576_length_1084_cov_4.015549, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001239 DAOQOC010000000
VERSION     DAOQOC010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135576_length_1084_cov_4.015549"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PL106_15840"
     CDS             complement(<1..1073)
                     /locus_tag="PL106_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34601.1"
                     /translation="MRLNAGDCASYWHANLVPNVYELDDYQVRTPTDIIGQHIHLVKF
                     DVTSSDGSANGFNYEDGTLSPMEVQERIHAITANGCSNFECPQAEVHPFFGAGPDHNG
                     DGVGDWVGARTTVQRWYADAMLNSSWDNGLGTVFTHDHYGPSTHQQVGLYATVLIEPT
                     GAQYRDPESGKMMGTRHDGGPTSWRADIINIYDSTGRDMSHREFYFEFADFQHAYLKG
                     GGDRTMVDNGDGVSIPSYADFPNAINPSFRVQPPPGYEASLVYFPPICPDGSPRPCPE
                     AISGDDPGTYVVNYRNEPIAERVYDPATKKQAAGQAGDLAYAFSSLITRAIPELNLPF
                     NAYPPLTQGVDPSDPATPLLRVY"
BASE COUNT          215 a          283 c          358 g          228 t
ORIGIN      
        1 atgtacacgc gcaggagcgg tgtggccgga tcacttggat cgactccctg cgtgagaggc
       61 ggataggcat tgaagggcag gttgagttcc gggatggcgc gggtgatcag cgacgagaac
      121 gcataagcca gatcaccggc ctgcccagcc gcctgtttct ttgtagccgg gtcgtatacg
      181 cgctcggcga tgggttcatt gcgatagttg actacatacg tgccaggatc atcgcctgaa
      241 atggcttcag ggcacgggcg cggtgaaccg tcagggcaga tgggtgggaa gtaaaccagc
      301 gatgcttcat accccggcgg tggctggacc ctgaacgagg gattgatcgc gttcgggaag
      361 tcggcataag agggtatgga tactccatcg ccattgtcca ccatggtgcg atctccgccg
      421 cccttgaggt aggcgtgctg gaagtctgcg aattcaaagt agaactcgcg gtggctcata
      481 tcccggccgg tcgagtcata gatgttgata atatccgcgc gccaggaagt ggggccgcca
      541 tcatggcgag tgcccatcat cttgccgctt tcagggtcgc ggtattgggc gccggtgggt
      601 tcgatcaaca ctgtggcgta aaggccgact tgttggtgag tggaaggacc atagtggtcg
      661 tgcgtgaaga ccgtacccag gccgttatcc caggaagagt tcaacatcgc atcggcatac
      721 cagcgttgca ctgtggtgcg cgcaccgacc cagtcaccga cgccgtcgcc gttatgatcc
      781 ggccctgccc caaagaaggg atggacttca gcctgcggac attcgaaatt gctgcatccg
      841 ttcgcggtga tggcatggat gcgctcctgt acttccatcg ggctgagtgt gccgtcttcg
      901 tagttaaagc cgttcgcgga accgtcggag gaggtcacat cgaacttgac gaggtgaata
      961 tgctggccga tgatgtcggt gggggtgcgg acctgataat catccaactc gtatacgttc
     1021 gggacgaggt tggcgtgcca atagctggcg cagtccccgg cattgaggcg catgaccagc
     1081 ggct
//
LOCUS       DAOQOC010001240         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_136939_length_1079_cov_3.612305, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001240 DAOQOC010000000
VERSION     DAOQOC010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136939_length_1079_cov_3.612305"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="PL106_15845"
     CDS             complement(<1..>1079)
                     /locus_tag="PL106_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HNA34602.1"
                     /translation="NITVGVVYARASSGGAWASVLALRKADDKAQALFDNCFRILDGP
                     DAPDLDIEELDRELILYLTNPNGSNNNVNYPEDYAELDPTIPEDASDRYYHFQGYQVY
                     QVRDGDVGVADVGNEDLVRLLGVYDLDDGVDRLINWIYDQDMGVPVATEMVNAPDTGV
                     VHSIKVTEDLFATGDKRLVNYKTYYYMALAYGYNNFQPYNPDPNQQSGQAYPFKAGRK
                     SASGAIRSWSGIPHPPASELGGTIQNAQYGDQFAITRLEGQGNGGLAVAITRETEDAI
                     VASPDNRMQEVKYLAGFGPVKVKVVDPLRVPDAHFELYLKDTTSLPQLGSSFNNQYTR
                     MNDAWWTLVRINPDGSTQTVDADRS"
BASE COUNT          187 a          323 c          342 g          227 t
ORIGIN      
        1 gctccgatcg gcgtccacgg tttgggtgga tccatcgggg ttgatgcgca ccagtgtcca
       61 ccatgcgtcg ttcatccggg tgtactggtt gttgaacgag gagccgagct ggggcaagct
      121 ggtggtatcc ttcaggtaga gttcgaagtg ggcatcgggc acgcgcagcg gatcgaccac
      181 tttcaccttg accgggccaa agcctgcgag gtacttcacc tcctgcatgc ggttgtccgg
      241 actggcgacg atggcatcct cggtttcgcg ggtgatggcc acagcaagac caccgttgcc
      301 ctgaccttcc aggcgggtga tggcgaactg atcgccgtac tgcgcattct gaatggtacc
      361 gcccaattca ctggccgggg gatgcgggat gccgctccac gagcggatgg cgcccgaagc
      421 cgacttgcgg cccgccttga agggatacgc ctggccgctc tgctgattgg ggtcgggatt
      481 gtatggctgg aaattgttgt agccgtaagc gagcgccatg tagtagtacg tcttgtagtt
      541 caccaggcgc ttgtcgcccg tggcaaaaag atcctcggtc accttgatgg aatgcaccac
      601 cccggtgtcg ggtgcgttca ccatctccgt ggccacgggc acgcccatgt cctgatcgta
      661 gatccagttg atcaggcggt ccacgccatc atccaggtcg tacacgccca gcaggcggac
      721 caggtcctcg ttgcccacat cggccacgcc cacatcacca tcgcgcacct gatagacctg
      781 atagccctgg aagtggtaat agcggtcgct ggcatcctcg gggatggtgg gatccagttc
      841 ggcgtaatcc tccggatagt tcacgttgtt gttgctgccg ttcgggttgg tcaggtacag
      901 gatcagctcg cggtccagct cctcaatgtc gaggtcgggt gcatcgggac cgtccaggat
      961 gcggaagcag ttgtcgaaca gggcctgggc cttgtcgtcg gccttgcgca gcgccagcac
     1021 actggcccaa gcaccaccgc tgctcgcccg tgcgtacacc acaccaacgg tgatgttgt
//
LOCUS       DAOQOC010001241         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_137044_length_1079_cov_2.379883, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001241 DAOQOC010000000
VERSION     DAOQOC010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137044_length_1079_cov_2.379883"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PL106_15850"
     CDS             <1..511
                     /locus_tag="PL106_15850"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34603.1"
                     /translation="IMAHWPSRRGGEKRSLPKRALAAQLGRHLVDSLLALNKDARIIY
                     MGDLNDDPVDPSVRRFFGSTDDKARVSNATFFNPMQPLYEKGIGSLAWQDSWNLFDQF
                     MLSPALVTSKKGYQYYGVRVFNEPYLAQKEGNFKGYPFRIYVGDTFTNGFSDHFPVFI
                     ILAKQVPKP"
     gene            604..1020
                     /locus_tag="PL106_15855"
     CDS             604..1020
                     /locus_tag="PL106_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34604.1"
                     /translation="MRHLLLPLLVLHSITATAQTVDSLHIFRSIPAGRYTTSSAETTA
                     WKLAHEGASFVFLGPAELDGVNHELGSQQPEAHNHTTLPGLSHIGFVYYGKRAHAFCL
                     SEETGLLVDLTARRQVRMTDTLTRMKLKAALLALGL"
BASE COUNT          201 a          380 c          313 g          185 t
ORIGIN      
        1 catcatggcg cactggccca gccgacgcgg gggcgagaag cgcagcctgc ccaagcgcgc
       61 gctcgccgct caactgggcc gtcatctggt cgattcgctc ctcgccctga acaaggacgc
      121 ccggatcatt tacatgggcg acctgaacga cgaccccgtg gacccgagtg tgcgtcgctt
      181 cttcggctcc accgacgaca aggcccgggt gagcaacgcc acgttcttca atcccatgca
      241 gccgctctac gaaaaaggca tcggctcgct ggcctggcag gactcgtgga acctcttcga
      301 ccagttcatg ctctccccgg cgctcgtcac cagtaaaaaa ggataccaat attatggtgt
      361 tcgcgtcttc aacgaaccgt acctggccca gaaggaaggc aacttcaagg gctatccgtt
      421 ccgcatctat gtgggggaca cgttcaccaa cggcttcagc gaccacttcc cggtgttcat
      481 catcctggcg aagcaggtgc ccaagccctg atggggtcca gcatgtggcg gaggtgcgag
      541 ggcacgaagc tgcgggaacg gccgggccgc cactctcccg ccccccggca tccgttctcc
      601 ccgttgcgcc acctcctcct tccgctcctc gtcctccaca gcatcactgc caccgcgcaa
      661 acggtggaca gtctgcacat cttccgttcg atccctgcgg gacggtatac cacctccagc
      721 gccgagacca ccgcgtggaa actggcacat gagggagcct cgttcgtctt cttgggccct
      781 gccgagctgg atggcgtgaa ccacgaactg ggatcccagc agccggaagc gcacaaccac
      841 accacgttgc ccgggctttc gcacatcggc ttcgtgtact acggcaagcg ggcgcacgca
      901 ttctgcctgt cggaagaaac cggcctgctg gtggacctca ccgcgcggcg gcaagtgcgc
      961 atgacggaca ccctgacgcg catgaagctg aaggccgcac tgctggcgct ggggctgtag
     1021 ggacgggccc acttcgcggg taccacgccc cgaagccacc ggtccttcca cgccgggat
//
LOCUS       DAOQOC010001242         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_137482_length_1077_cov_4.529354, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001242 DAOQOC010000000
VERSION     DAOQOC010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137482_length_1077_cov_4.529354"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PL106_15860"
     CDS             complement(<1..355)
                     /locus_tag="PL106_15860"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNA34605.1"
                     /translation="MRYALIATTLFLCLAFVPKKDKSRERYVYDNEQDFTAQEIQRLD
                     TLFRGHEARTGNEIVLVTTASYLGWPTIFEFAGTVGDSLRVGKRGRNNGVIIAFSKER
                     REVFIATGLGTEQVMS"
     gene            complement(415..>1077)
                     /locus_tag="PL106_15865"
     CDS             complement(415..>1077)
                     /locus_tag="PL106_15865"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNA34606.1"
                     /translation="EAFGKLGVKDIDVLDIRDRKDVRPEMIKRLSRADGVMMSGGNQL
                     RLTTIFGGTAFLQLMKRRYEEENGFVIAGTSAGAMCMSSTMIYQGHSASGLIKGGVKM
                     TTGAGLIQDVIIDSHFVERGRFSRLTQAVASNPNSIGIGLGEDTGVVITGSDMLETIG
                     SGQVMIFDGHDITYSDYADVEEGEPFSIEGMRVHIISRGHSYSVKERMFTAVKVHGLS
                     AK"
BASE COUNT          191 a          355 c          299 g          232 t
ORIGIN      
        1 cggacatcac ttgttcggta cccagaccag tggcaatgaa aacttcgcgc cgctctttgc
       61 tgaaggcgat gatcaccccg ttgttccgcc cacgcttgcc cacgcgaagc gaatcgccca
      121 cggtaccagc gaactcgaaa atggtgggcc agccgaggta gctcgccgtg gtcaccagta
      181 cgatctcgtt ccccgtgcgc gcttcgtggc cccggaacaa ggtgtccagt cgctggatct
      241 cttgagccgt gaagtcctgc tcgttatcgt aaacgtaacg ctcccgactc ttgtccttct
      301 tggggacgaa agccaggcac aagaagaggg tggtggcgat cagggcgtaa cgcatgggac
      361 caagttagga agtgccagca cgcgttcaaa gttccctgcc cggcgggacg ggtcttactt
      421 cgccgaaagt ccgtggacct tcaccgcggt gaacatgcgt tccttcacgc tgtaactgtg
      481 gccacgactg atgatatgca cccgcatgcc ttcgatgctg aacggctcgc cttcctccac
      541 gtccgcgtag tcgctgtatg tgatgtcgtg tccgtcgaag atcatcacct ggccgctgcc
      601 gatggtctcg agcatgtccg accccgtgat caccaccccg gtgtcctcgc cgagtccgat
      661 gccgatgctg ttggggttgc tggccaccgc ctgggtcaat cggctgaacc ggccgcgctc
      721 cacgaaatgg ctgtcgatga tcacatcctg gatcaggccc gcgccagtgg tcatcttcac
      781 ccctcctttg atcagaccac tggcgctgtg gccctggtag atcatggtgc tgctcatgca
      841 catggccccg gcgctggtgc ccgcgatcac gaatccgttc tcctcctcgt aacgccgctt
      901 catcagttgc aggaaggccg taccgccgaa gatggtggtg agccgcaact ggttgccgcc
      961 gctcatcatc acgccgtccg cacggctcaa ccgtttgatc atctcgggcc gcacgtcctt
     1021 gcgatcccgt atgtccagta catcgatgtc ctttacgccc agcttcccaa aggcctc
//
LOCUS       DAOQOC010001243         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_137791_length_1076_cov_4.226249, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001243 DAOQOC010000000
VERSION     DAOQOC010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137791_length_1076_cov_4.226249"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1076)
                     /locus_tag="PL106_15870"
     CDS             complement(162..>1076)
                     /locus_tag="PL106_15870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA34607.1"
                     /translation="KSMLEQGEAGPYLNAALIRARMRDPDGNVAAGLAEPLMKNTSAP
                     VERLAAFESAFDVYPERSTARIELLQQVFMPPYDPGLVAAASERLINEEPGTISVVLK
                     DAMLAPVRNALHPIRDLETRQLLDAVVARRDGMDTPVHASPPFNHPIDSVRLAHITTG
                     TRYRIVTSHGSVVVELEPEAAPGSCVAFDSLVSAGYYNGKFFHRVVPDFVAQGGCPRG
                     DGYGGMPWSLRTEVGLQGFTTGAVGLASAGKDTESCQFFIMLADAPHLDGRYTRFAHV
                     VSGMDVAWKLRVGDGIQQVERIGAVTAP"
BASE COUNT          202 a          353 c          307 g          214 t
ORIGIN      
        1 cgatacgaac cggatcttcg gctatgatcg ggactggctg atcgccttct ccgcgaacaa
       61 gcccgacttc actggcctct gcgtctcgtt ggatctgcgt ggcgggtctc caacactgaa
      121 cacatccctg atccgcgacg gcattgaacg gccggagtgg ctcacggtgc ggtgaccgcg
      181 ccgatccgtt ccacctgttg gatcccatcc cccacccgca gcttccacgc cacgtccatt
      241 ccgctcacca cgtgcgcgaa gcgcgtatac cgaccatcga gatgcggtgc atcggcgagc
      301 atgatgaaga actggcaact ctcggtatct tttccagccg aggccagtcc gaccgccccg
      361 gtggtgaagc cttgcaagcc gacctccgtg cgcaggctcc agggcatgcc cccgtagcca
      421 tcgccacgcg ggcagccgcc ttgcgcaacg aaatcgggca ccacgcggtg gaagaacttg
      481 ccattgtagt accctgcgct caccaacgag tcgaaagcga cgcaactgcc gggcgctgct
      541 tccggttcca actcaactac cacactccca tgtgaagtga cgatccgata ccttgttccg
      601 gttgtaatat gcgcaagacg tacgctgtcg atcgggtggt tgaacggtgg cgaagcatgc
      661 accggtgtat ccatgccatc acgcctggca acaaccgcat caaggagctg tcgtgtttcc
      721 aggtcgcgga tcggatgaag tgcgttgcgc acaggcgcga gcatcgcatc cttcaataca
      781 acggaaatgg tacctggttc ctcgttgatc agacgttcgc tcgcggctgc gacaagtccg
      841 gggtcgtacg ggggcatgaa tacctgttgg agcagttcga tccgggcggt actcctttcc
      901 ggatacacgt cgaatgcgct ctcgaatgcc gccagccgct ccacgggcgc ggacgtgttc
      961 ttcatcaacg gttccgcgag gcccgccgcc acgttcccat cgggatcccg catgcgggcc
     1021 ctgatcagcg cagcattgag gtaaggtccg gcctcgccct gttctagcat gctttt
//
LOCUS       DAOQOC010001244         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138151_length_1075_cov_2.807843, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001244 DAOQOC010000000
VERSION     DAOQOC010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138151_length_1075_cov_2.807843"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1057
                     /locus_tag="PL106_15875"
     CDS             167..1057
                     /locus_tag="PL106_15875"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNA34608.1"
                     /translation="MKHWLHAFRLRTLPLAVSSILVGSALASFHGAFHWPVLAFALLT
                     AVLLQILSNLANDLGDHQHGTDNVERIGPQRAVQSGAIAPDIMKRGMLVCGSLAFLSG
                     CALITVALGFTWGTLIFLLLGLLAIGAAVKYTFGKDPYGYAGYGDASVFLFFGIVGVC
                     GTFCLHTRVFFPAVLLPAVAFGLLSTGVLNVNNMRDIANDAASGKRTLVVRMGGANAR
                     VYHTLLVLGGIAGLVGFAVFTRDRSTLLPLLAAPVFLWHLRQVWTNTEPRLLDPQLKV
                     LALGTFLTALLFAAGLIFAA"
BASE COUNT          164 a          335 c          337 g          239 t
ORIGIN      
        1 attcgcccgc aacggcccat tcaccattca ccaactccca ttcacccgca ttcgtattca
       61 ccccgcgcgg caaggtgcag gccaagggca agggggaact ggagatgtat ttcgtggagc
      121 gcaaggcttg aagacggtcg tgcgcgttgg tgttcctttg cccggcatga agcactggct
      181 ccacgccttc cgcctgcgca cacttccgct cgcggtgagc agcattctgg ttggaagtgc
      241 actcgcctct ttccacggtg cgttccattg gcccgtgctg gcgtttgccc tgctgaccgc
      301 ggtgttgttg cagatcctca gcaacctggc caacgacctg ggcgaccacc agcacggtac
      361 ggacaacgtg gagcgcatcg gcccgcaacg tgcggtgcag agcggggcga tcgccccgga
      421 catcatgaag cgcggcatgc tggtgtgcgg atccttggcc ttcctgagtg gctgcgcatt
      481 gatcacggtg gcgctcggct tcacatgggg caccctgatc ttcctgctgc tcggtctgct
      541 cgccatcggt gccgcggtga agtacacctt cgggaaggat ccgtacggct acgccgggta
      601 tggcgacgcg agtgtgttcc tgtttttcgg tattgtcgga gtatgtggaa ccttctgcct
      661 ccacacccgc gtgttcttcc cggccgtgct gctgccggcg gtggccttcg ggctgttgag
      721 cacgggtgtg ctcaacgtga acaacatgcg cgacatcgcc aacgatgcgg ccagtggaaa
      781 gcgcacgctg gtggtgcgca tgggcggtgc caacgccagg gtgtaccaca ccttgctcgt
      841 gctgggtggg atcgccggac ttgtcggctt cgcggtgttc acccgggatc ggtcaacctt
      901 gctgcctttg cttgccgcac cggtttttct ctggcatctt cgccaggtct ggacgaacac
      961 ggaaccccgg ttgttggatc cccagttgaa agtactggcc ttgggcacct tcctcaccgc
     1021 gctgctgttc gccgcaggtc tcatcttcgc ggcatgatgc aggcagcttg gaaag
//
LOCUS       DAOQOC010001245         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138362_length_1074_cov_3.785083, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001245 DAOQOC010000000
VERSION     DAOQOC010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138362_length_1074_cov_3.785083"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PL106_15880"
     CDS             <1..985
                     /locus_tag="PL106_15880"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNA34609.1"
                     /translation="EPTTDTWTQKAPLPGGARQYASAFTIGDTGYVLCGWDNVNEIDY
                     NDLWAYDPATDTWEQKASLPAPPRYAGVAFSIDGIGYYGTGAGNAIYYNDFHAYDPVT
                     DTWMQKADFPGNARRWAVGFAVDGRGYIGTGESSGVRYRDFHAYDPLTDTWQQRADLG
                     STNRRNGYAFMVDGHAYAGGGQDFTQNWNDFHRYDVGTDSWVPVSAFPLNACKNGASF
                     STGNAAFIVGGNGQLPVLDEIWRFLPDITTGSIEHRFNTHLLFPNPVNDRLWIKAPEK
                     ARYVIHDPCGATVAAGQVGTSIDMDRLPEGLYTLIVEGGEERSVERFMIAR"
BASE COUNT          236 a          308 c          327 g          203 t
ORIGIN      
        1 cgaacccacc acggacacct ggacacagaa ggccccgctg cccggtggtg cgcgccagta
       61 cgccagcgcc ttcaccatcg gcgataccgg atatgtactg tgcggttggg ataacgtgaa
      121 cgagatcgac tacaacgacc tgtgggcgta cgaccctgct accgacactt gggaacaaaa
      181 ggcctccctg ccggcaccac cgcggtatgc gggcgtggcg ttcagtatcg atggcatcgg
      241 ttactacggc acaggtgcgg gtaatgccat ctactacaac gacttccacg cctacgatcc
      301 cgtcacggac acttggatgc agaaagccga ctttcccggc aatgcacggc gatgggccgt
      361 gggctttgct gtggatggca ggggctacat cggcacaggc gaaagcagcg gtgtgcgata
      421 ccgcgacttc catgcctatg atcccttgac cgatacctgg caacaacgcg ccgacctcgg
      481 cagtaccaac cggcgcaatg gctacgcctt catggtggat ggccacgcgt atgctggcgg
      541 cggccaggat ttcacgcaga actggaacga cttccaccga tacgatgtag gtaccgatag
      601 ctgggtgccc gtaagcgcct tcccgctgaa tgcctgcaag aacggagcga gtttcagtac
      661 gggcaatgcc gccttcattg tgggcggtaa cggacaattg cccgtgttgg acgagatctg
      721 gcgttttctg ccggacataa caacaggatc gatcgaacac cgcttcaata cacacttgct
      781 cttccccaac ccggtgaacg accggttgtg gatcaaggca ccggagaagg cacggtatgt
      841 gatccatgat ccttgcggag caacagtagc tgcgggccag gtcggcacca gcattgatat
      901 ggaccgtctt ccggaaggcc tttatacgct catcgttgaa ggaggtgagg aacggtccgt
      961 ggagcgtttc atgatcgcgc gctgatagca gggccttcaa cgccgcagct ttactgaggc
     1021 ggtgacgaag atccggtcgc tgcggtagac aacggtgtgg tgaagaagtg agga
//
LOCUS       DAOQOC010001246         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138370_length_1074_cov_3.557409, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001246 DAOQOC010000000
VERSION     DAOQOC010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138370_length_1074_cov_3.557409"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PL106_15885"
     CDS             complement(<1..753)
                     /locus_tag="PL106_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA34610.1"
                     /translation="MAHGLLKGKRGIISGALNEQSIAWKVAELAHAEGATIVLTNAPV
                     AMRMGEIDKLAEAVKAPIIGADATKDEDLERLFAEGQQKLGGKVDFLLHSIGMSPNVR
                     KGKAYEDLNYDWFKQTLDISAMSLHRMVQAAFKQDAMAEWGSIVALSYIAAQRVFPGY
                     GDMADAKALLESIARSWGYYYGQKNKVRVNTVSQSPTPTTAGSGIKGFGGFYGFAQEM
                     SPLGNAPAVDCANYCITLFSDLSRYVTMQNLFH"
BASE COUNT          184 a          319 c          329 g          242 t
ORIGIN      
        1 gtggaagagg ttctgcatgg tcacgtaacg gctcagatca ctgaacaagg tgatgcagta
       61 gttcgcgcag tccaccgcag gagcgttgcc cagcgggctc atctcctgag cgaaaccgta
      121 gaaaccgccg aatcccttga tgccgctgcc ggccgtggtg ggtgtggggc tctggctcac
      181 ggtattcacg cgcaccttgt tcttctggcc gtagtagtag ccccaactgc gcgcgatact
      241 ttcgagcaac gccttcgcat cggccatgtc gccatatccg gggaacacgc gttgggcggc
      301 gatgtagctg agggccacga tgctgcccca ttccgccatg gcatcctgct tgaaggcggc
      361 ctggaccatg cggtgcaggc tcatggcgct gatgtcgagc gtctgcttga accagtcgta
      421 gttgaggtcc tcgtatgcct tgcctttgcg cacgttgggg ctcatgccga tgctgtgcag
      481 caggaaatcg accttgccac cgagcttctg ctggccttcg gcgaagagcc tttcgagatc
      541 ctcgtcctta gtggcatccg cgccgatgat gggcgccttc accgcctcgg ccagtttgtc
      601 gatctcgccc atgcgcatgg ccacgggagc gttggtgagc acgatcgtcg cgccttcggc
      661 atgggccagt tcggccacct tccaggcgat gctctgctcg ttcaacgcgc cgctgatgat
      721 accgcgcttg cctttcaaca gtccgtgggc catgttcgat cggttatggg ccgaagatag
      781 aaggcgcttc ggatgcggtt atggagccgg tgagaaagaa aaggacggtt caggggtgca
      841 ataccatcta cacattcaat atcgcccgat ccatatccca gcatccgctt tgtccatttg
      901 atgagggagg cctgttccga acgcgatccg cccctgaagg tggaaacgct cgacctgaac
      961 ggcatcgcgg accggttcaa agccgtggtg ctgcgtgatg gcgatccgct gtggtgatca
     1021 gcgccccttg agcagttcgc gagcgttctc ctccgcggct ttgctggtct tttt
//
LOCUS       DAOQOC010001247         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138401_length_1074_cov_3.099117, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001247 DAOQOC010000000
VERSION     DAOQOC010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138401_length_1074_cov_3.099117"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>1074
                     /locus_tag="PL106_15890"
     CDS             34..>1074
                     /locus_tag="PL106_15890"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA34611.1"
                     /translation="MHGTLHGELIIVLALAVGILLLFRRLKLPNVLGFIIAGLLAGPH
                     ALGWVAEVETVETLAELGVIFLLFVIGMEFSLKKLASISATVFIGGSLQAVLTTALTA
                     GIGRLLGLAMPTAVFTGFLVTLSSTAIVLRILQEQGRTDQPFGRITAAILIFQDIIVV
                     PMMLVTPMLAGQSSNIGHDLLMLVLKMLLLVVAVYLLGRFGVPRLLRAVSKGRNKELF
                     IITIVVLCFSAAWATSALGLSLALGAFFAGLIISETDQVYHATGIVQPFHELFMSFFF
                     VSIGMLVDPALFAHAPFTIIGLALAVAVVKTLAAFLAIWVLRHPVATALKCGFALFQI
                     GEFAFVLAITGLD"
BASE COUNT          159 a          334 c          345 g          236 t
ORIGIN      
        1 gtagcggtcc ggcgcgtatt caaggattga gcgatgcacg ggaccctcca tggtgagctg
       61 atcattgtcc tggcactggc cgtggggatc cttctgctct tccgccgatt gaaactgccg
      121 aacgtactgg ggttcatcat cgccggcctg ctggcggggc ctcatgcgct gggctgggtg
      181 gccgaggtgg agaccgtgga gaccctggcc gagctgggcg tgatcttcct gcttttcgtg
      241 atcggcatgg aattcagcct gaagaaactg gcgagcatca gtgccacggt gttcatcggt
      301 ggctcactgc aggccgtgct caccacggcg ctcacggcgg ggatcggacg gctgctcggc
      361 ctggccatgc caaccgccgt gttcacgggc ttcctggtca cgctcagcag cacggccatc
      421 gtattgcgca tcctgcagga gcagggccgg acggaccaac cattcggccg catcaccgct
      481 gccatcctta tttttcaaga tattatcgtg gtacccatga tgctcgtgac cccgatgctg
      541 gccgggcaga gcagcaacat cgggcacgac ctgttgatgc tggtgctgaa gatgctcctg
      601 ctggtggtcg cggtgtacct gctgggccgt ttcggagtgc cgcgcctgtt gcgtgcggtg
      661 agcaaagggc gcaacaagga actgttcatc atcaccatcg tggtgctgtg cttcagtgcg
      721 gcctgggcca cgagcgcgtt gggcctctcg ctggcgttgg gcgccttctt cgcgggcctc
      781 atcatcagcg aaacggacca ggtgtaccat gccacaggga tcgttcagcc cttccatgaa
      841 ctgttcatga gcttcttctt cgtgagcatc ggcatgctcg tggatcccgc gctgttcgcg
      901 cacgcgccgt tcaccatcat cggcctggcc ctggccgtgg ccgtggtgaa gacgctggcg
      961 gccttcctgg ccatctgggt gctgcgccat ccggtggcca ccgcgctcaa gtgcggcttc
     1021 gccctcttcc agatcggcga gttcgccttt gtgctcgcca tcaccgggct ggac
//
LOCUS       DAOQOC010001248         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138481_length_1074_cov_2.388616, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001248 DAOQOC010000000
VERSION     DAOQOC010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138481_length_1074_cov_2.388616"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PL106_15895"
     CDS             <1..1029
                     /locus_tag="PL106_15895"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNA34612.1"
                     /translation="DIPANMPPMPIPPDNPLTEEGIALGRFLFYEERLSGDNTMSCAQ
                     CHAPAHAFTDGNAVSVGIDGIAGNRSSMPLINLGYAQSFFWDGRVATLEEQILNPVRN
                     PIEMHEIWPDVVSKLQADTAYVKLFWDAFGTTTVDSLLAAKAVAQFLRTLISANSPWD
                     KWRRFEGTIPLDAQIGYDLFKMEGGPVGMPFQLPGMDSTIIGQGGADCFHCHVEAAGL
                     FTDEQFHNNGLDSEPFADLGRGGFNNDPFDNGKFKTPTLRNIMLSAPYMHDGRFATIE
                     EVLDHYNDGGHPSSTVDPFMKFTDPDLTLELDATKRAQILAFLNTLTDTEFIENPAFA
                     DPGPPHWP"
BASE COUNT          216 a          344 c          317 g          197 t
ORIGIN      
        1 gacatcccgg ccaacatgcc gccgatgccc ataccacctg acaatccgct cacggaggaa
       61 ggcattgccc tgggccgatt cctgttctat gaggaacgcc tcagcgggga caacaccatg
      121 agttgcgcgc aatgccacgc tccggcccat gcgttcaccg atggcaatgc ggtcagcgtg
      181 gggatcgatg gcatcgctgg taaccggtcc tccatgccgc tcatcaacct cggctatgcg
      241 cagagcttct tctgggacgg ccgcgtggcg accttggagg agcagatcct caatccggtc
      301 cggaatccca tcgagatgca cgagatctgg cccgatgtcg tatccaaact gcaggccgat
      361 accgcgtacg tcaagttgtt ctgggatgcg ttcggaacga ccaccgtgga ctccttgctc
      421 gccgcgaaag cggtcgctca gttcctgcgt acactgatca gcgcgaacag tccctgggac
      481 aagtggcggc gcttcgaggg caccattccg ctggatgccc agatcggcta cgatctgttc
      541 aagatggaag gcggtccggt gggcatgcca ttccagttgc ccggtatgga ttcaacgatc
      601 atcgggcagg gaggcgcgga ttgtttccac tgccacgtgg aggcggccgg gctgttcacc
      661 gatgagcagt tccacaacaa cgggctggac agcgaaccct tcgctgacct gggccgcggc
      721 ggcttcaaca acgatccctt cgacaacggg aagttcaaaa cgcccacgct gcgcaacatc
      781 atgctttccg caccctacat gcacgatggc cgtttcgcca ccatcgagga ggtgctggat
      841 cactacaacg acggcgggca tccgagttcc acggtggatc ccttcatgaa attcaccgac
      901 ccggacctga ccctggagtt ggacgccacg aagcgcgcgc agatcctggc cttcctgaat
      961 acgctcacgg acacggagtt catcgagaac cccgcgttcg ccgatcccgg accgccgcat
     1021 tggccgtagg aacgggaccg gcccggtcca tgtgtgcgtt ccggaacagg taag
//
LOCUS       DAOQOC010001249         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138613_length_1073_cov_4.166012, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001249 DAOQOC010000000
VERSION     DAOQOC010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138613_length_1073_cov_4.166012"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PL106_15900"
     CDS             complement(<1..778)
                     /locus_tag="PL106_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA34613.1"
                     /translation="MRACVFSVDIEDWFHILDLPSTPKLEEWPALQSDVVRNTRRMYA
                     TFRKHDVRCTLFFLGWVAERWPDLVKEAVDDGHEIASHGYAHELVYTITEQRFSDDIR
                     KAREIIERAGGKVVKGYRCPGFSVTDATPWFFDRVREAGYLYDSSVWPGARGHGGLPG
                     AKATPHIITTGHGDLVEFPISMARTLGRPMYFFGGGYLRFFLYALIRSKVKQVLAEDR
                     PVAVGRTEAGGSPGAGIGHPIRLGGNVAGVVVVEVSPCPEA"
     gene            complement(884..>1073)
                     /locus_tag="PL106_15905"
     CDS             complement(884..>1073)
                     /locus_tag="PL106_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34614.1"
                     /translation="YGAIPKITLLDMKGFFDKEIKGRPYTYCVIGKESGMDMKALERL
                     GPVRKLTKKELFGYDEER"
BASE COUNT          192 a          359 c          314 g          208 t
ORIGIN      
        1 gggcttccgg acagggcgac acctcgacga cgacgacgcc ggccacgttc ccccccagcc
       61 ggatcggatg tccgatgccg gccccggggg aaccgcccgc ctcggtgcgc ccgacggcca
      121 cgggccggtc ctcggcgagc acctgcttca ccttgctccg aatgagcgcg tacaggaaga
      181 aacgcaggta gccaccgccg aagaagtaca tgggtcgccc cagtgtacgc gccatgctga
      241 tggggaactc caccaggtcg ccatgcccgg tggtgatgat atgcggcgtg gctttcgcac
      301 cgggcagacc accatgtcca cgtgctcctg gccaaacact gctgtcgtag aggtatcccg
      361 cctcgcgcac ccggtcgaag aaccagggcg tggcatcggt cacgctgaaa ccaggacacc
      421 ggtagccttt cacgaccttc cctcccgctc gctcgatgat ctccctggcc ttgcggatat
      481 cgtcggagaa ccgttgttcg gtgatcgtgt acaccagttc gtgcgcgtag ccatggctgg
      541 cgatctcgtg gccatcgtcc acggcttctt tcaccaggtc gggccagcgt tcggccaccc
      601 aacccaggaa aaagagtgta cacctcacat cgtgcttccg gaaggtcgcg tacatccggc
      661 gcgtgttgcg caccacatcg ctctgaagcg cgggccactc ctccaacttc ggggtgctgg
      721 gcaggtcaag gatgtggaac cagtcctcga tgtcgacgga gaagacgcag gctctcatgt
      781 ggatggcgag tggcgagcgc tgagtggcga gagacgagtg gcatgatgcg ggatgccact
      841 cgtctctcgc cactcgtcgc tcagcactcc ttgcaaggat cgttcacctc tcctcatcat
      901 acccgaagag ttccttcttc gtcagcttcc gcacgggtcc cagcctctcc aaggccttca
      961 tgtccatgcc gctttccttg ccgatgacgc aataggtata gggccttccc ttgatctcct
     1021 tgtcgaagaa acccttcatg tccagcaggg tgatcttcgg gatcgccccg tag
//
LOCUS       DAOQOC010001250         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_138754_length_1073_cov_2.317289, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001250 DAOQOC010000000
VERSION     DAOQOC010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138754_length_1073_cov_2.317289"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PL106_15910"
     CDS             <1..199
                     /locus_tag="PL106_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34615.1"
                     /translation="VPERLRWIAELNPLYHFIALVRSILVEGAMPTTGEWGTVVFLAT
                     VAMAVGLLVHRWLRKGYVANY"
     gene            complement(213..>1073)
                     /locus_tag="PL106_15915"
     CDS             complement(213..>1073)
                     /locus_tag="PL106_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34616.1"
                     /translation="ALRRKWPVPGSIGLALLFLMSQGTNVFYYAFRSPAYSHIGSWVL
                     VCLALWCWAHGVSGRDGRWRGALFRIACAWLILIRPLDLLLVLALYALVLADEPFTRR
                     PRWFLGQIVIGIALALPQMFYWQAAYGDPIVFSYQNEGFTNWRTPHLLATLLAPKNGL
                     LPYAPAFLLLPVAVLAAWHRERTRTIIVLFMFLLYWYAVSSWWDPAFGCSFGQRSFVQ
                     TVPLLAMITWCAVHHWWPRRPAIVLLLLAPFLAAAFINNMLAHLYDMCYWSTTWDPTQ
                     WLGNIRQVFN"
BASE COUNT          245 a          290 c          367 g          171 t
ORIGIN      
        1 ggttcccgaa cgcttgcgtt ggatcgccga gctgaatcca ttgtaccatt ttatcgcgct
       61 ggtgcgctcc atcctcgtcg agggcgccat gccgaccacg ggcgaatggg gcacggtggt
      121 gtttcttgca acggtggcca tggccgttgg gctgctcgtg caccgttggc tgcgcaaggg
      181 ctacgtggcg aattactaag gcgggtgagg attcagttga acacctgccg gatattcccg
      241 agccactgtg tcggatccca tgtggtagac cagtagcaca tgtcgtacag gtgtgcgagc
      301 atgttgttga tgaatgctgc ggccaaaaag ggagcgagca ggagaagcac gatcgcgggg
      361 cgtcgcggcc accagtgatg cacggcgcac caggtgatca tggcgagcag cggaacggtc
      421 tgcacgaagg accgctggcc gaagctgcac ccgaaggcgg ggtcccacca cgatgagacg
      481 gcgtaccagt atagtaggaa catgaaaaga acaatgatcg tcctggttcg ctcgcggtgc
      541 cacgcggcaa gaaccgcgac aggcaggagt agaaaggcgg gagcgtatgg cagcaaaccg
      601 ttcttcggcg caagtaaagt ggcgagcaag tgaggagtcc tccagttggt gaacccttcg
      661 ttctggtatg aaaagacgat cggatcccca taagcggcct gccagtagaa catctggggc
      721 agggccaagg cgataccgat gacgatctgc cccaggaacc aacgcgggcg gcgggtgaaa
      781 ggttcatcgg cgaggaccag ggcgtacagc gccagtacca gaaggaggtc cagtgggcga
      841 atgaggatga gccatgcaca ggcgatgcgg aaaagtgcgc cgcgccaccg gccatcacgg
      901 ccagatacgc cgtgggccca gcaccagagc gccaggcaga ccaacaccca cgaacctatg
      961 tgcgagtatg ccggggaacg gaaggcatag tagaagacgt tggtgccctg gctcatgagg
     1021 aagagcagcg ccaggccgat gctgccgggc acgggccatt ttcgccgcaa ggc
//
LOCUS       DAOQOC010001251         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139062_length_1072_cov_1.997050, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001251 DAOQOC010000000
VERSION     DAOQOC010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139062_length_1072_cov_1.997050"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PL106_15920"
     CDS             complement(<1..360)
                     /locus_tag="PL106_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34617.1"
                     /translation="MPLTPYHIGPHPSDDTIIWKYFKRPYFESLINDSALFFRRVIDF
                     VTDDPNEGTIPQRDQQRDRLLALSRPHPTISSLVSAVTVANGIDGWGMRHATVINCWT
                     MRPTESRMMWIAYAGIGD"
     gene            complement(676..1008)
                     /locus_tag="PL106_15925"
     CDS             complement(676..1008)
                     /locus_tag="PL106_15925"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNA34618.1"
                     /translation="MGRAVHPRTGALLRDDGEPVPGGGGAGAIGAAYLRGLSTHDQYI
                     QLSSLPEDVRRQVLDFIDFLMLRPDRTRKEPAKQNGKKPISGLLKGKIRIADDFDAPL
                     DDFKEYME"
BASE COUNT          191 a          316 c          311 g          254 t
ORIGIN      
        1 gtcaccgatt cctgcgtatg cgatccacat catgcgggat tccgtcggcc gcatggtcca
       61 acagttgatc actgtggcat gccgcatccc ccatccatcg attccgttcg ccacagtaac
      121 ggccgacacc agtgaactga tcgttgggtg cggccgggat agtgcgagca accggtcccg
      181 ttgctggtcc ctttgcggga tcgtgccttc attggggtca tccgttacaa aatcgatcac
      241 gcgcctgaag aacagtgcgc tgtcgttgat caggctttcg aaatacggcc ttttgaaata
      301 cttccagatg atggtatcat ccgatgggtg gggcccgata tggtatggtg taaggggcat
      361 gtgaggtggc attgaaggcg acaaacgtag cctcgtattc gctgcatgtg ttcccgcgct
      421 ttttcgtccg ccgcacctgg cccgtccgcg cttgcagccc tatttgtcgc gaatgttccg
      481 cgcgccttcc ccatagcggc ggaatagcgc atgcgggcca cttgtgacct tctgaatgag
      541 ccccgtgaat gccgggcgcg gtttcagacc gcgatgatcc ccgtccggca tgtgcgggtc
      601 acaagtggtt gcgccggagc gctggctcct gtcttgggcg cgaacacttg cgacaataga
      661 ggtcgagcag gatcctcatt ccatgtactc tttgaagtcg tcgagcggcg cgtcaaagtc
      721 atcggcgatg cggatctttc cctttagcag cccggagatg ggcttcttgc cgttctgctt
      781 cgcaggttcc ttccgtgtgc ggtccggtcg tagcatcagg aaatcgatga agtccaatac
      841 ttggcgtcgc acatcctccg gaagagagga cagttggatg tactggtcat gcgtgctcag
      901 gccacgaaga taagctgcac ctatcgcgcc cgccccccca cctcccggta ccggttctcc
      961 atcgtctcgt agtaaggctc cagttcgtgg gtgtaccgct cgtcccacac gaactggttc
     1021 tccttcttgg tgaagcgcgc gaagccgagc ggcttatcgc agatcgcgta at
//
LOCUS       DAOQOC010001252         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139169_length_1071_cov_4.230315, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001252 DAOQOC010000000
VERSION     DAOQOC010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139169_length_1071_cov_4.230315"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PL106_15930"
     CDS             <1..576
                     /locus_tag="PL106_15930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34619.1"
                     /translation="TALWTADGVPTIQESSYIPTPRIVASDSGAVIGIMDMTGPHKFC
                     AQRVHNDGSMAWAAPVSFSTGPQPFYDDRTELSDGSGGVVAFWETATGDLYGARIYRN
                     GLLYDDVDVAEHSSIHTIKAYPNPATDRVTLSVGNERVIRAEVVDATGRMHPVERDGA
                     SLNVHALAPGIHHVRLVCASGRVLRCSFIKW"
     gene            852..>1071
                     /locus_tag="PL106_15935"
     CDS             852..>1071
                     /locus_tag="PL106_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34620.1"
                     /translation="MLSPRSILGIGTAALLFPTLLHAQTTAPNSRWWTDISDRQVSTT
                     GERRIVPDVYRTVRINGTSLNALLEGARI"
BASE COUNT          207 a          359 c          320 g          185 t
ORIGIN      
        1 actgcgctat ggaccgccga tggtgtgccg accatccagg agagcagcta catccccacc
       61 ccgcgcatcg tggccagcga cagcggcgcg gtcatcggca tcatggacat gaccggcccg
      121 cacaagttct gcgcgcaacg cgtacacaac gacggcagta tggcctgggc ggccccggtc
      181 tccttcagca ccggaccgca accgttctac gatgatcgca ccgaactctc cgatggtagc
      241 ggtggcgtgg tggccttctg ggaaacagcg accggtgatc tctatggtgc gcgcatctac
      301 cggaacgggt tgttgtatga cgacgttgac gtggccgagc acagcagcat ccacacgatc
      361 aaggcctatc cgaacccggc caccgatcgg gtcacgttga gcgtgggcaa cgagcgcgtg
      421 atccgggccg aggtcgtcga tgcgacaggg cgaatgcatc cggttgaacg cgatggcgcg
      481 agcctcaacg tgcatgcatt ggcaccaggc atccaccatg tacgactggt ctgcgcttca
      541 ggccgtgtgt tgcggtgctc gttcatcaag tggtgaacga ccggtggaac ggtgacttga
      601 gccgatcgcc gctcaccatc gttccagcac gggtgcaaca ggcctccgtc cggggcgacc
      661 taccgcatca aggccagcta tccgcacaac ggtccggtgg ccttcagatc gaaggtccga
      721 gttcgtgcgg tgcatgcgcc gcgcggcgat cgaccctcaa cagcgcatcc ctcgacctcg
      781 ctccgtagga cgccacggca cgcccggcga attgcattgc ttcaaaccgg tcgatccccg
      841 taagatcgcg catgctttca cccaggtcca tcctcggcat cggcactgcc gcactgctct
      901 tccctacgtt gctacacgcc caaaccaccg cgccgaacag ccgatggtgg acggacatca
      961 gcgaccgtca agtgtccacg accggagagc gccggatcgt gcccgacgtc taccggactg
     1021 ttcgcatcaa cggaacatcg ctgaacgcgc tactcgaagg tgctcgtatc g
//
LOCUS       DAOQOC010001253         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139179_length_1071_cov_3.848425, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001253 DAOQOC010000000
VERSION     DAOQOC010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139179_length_1071_cov_3.848425"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PL106_15940"
     CDS             <1..883
                     /locus_tag="PL106_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34621.1"
                     /translation="PGFLSAGNYRMVYVDRMDRLERLHAQVMVRAGSSLKLWIGTERT
                     QRINTMGYRYVEQLGDGLSRYSDAFLYGALTLDLRWAYREKLARLQDRELTLETKYPV
                     LYANVSRAVKGLWGGQLDTWRASVLVEKNFRIRPLGRLSVQLLGGMADPRAPMPFLFN
                     LRGTYSTRDLLLAGDNAFETMRPNEFLADRYATLHLKYVLKWPLYRLKWSRPVPALVT
                     GLGLGALDRPQDHGGLPFSPMSGLYTESGIRLDGLLILNGLMHFGVGAYYRYGEHALP
                     TATDNWVFKLTAGLRLQ"
BASE COUNT          177 a          355 c          340 g          199 t
ORIGIN      
        1 acccggcttc ctatcggccg gtaactaccg catggtgtat gtggaccgca tggaccgcct
       61 cgagcggctc cacgcccagg tgatggtgcg cgccggaagc tccctgaagt tgtggatcgg
      121 caccgaacgc acgcagcgga tcaacaccat ggggtaccgg tacgtagagc aattgggcga
      181 tggcctcagt cgttactcgg acgccttcct gtacggtgcg ctcacactgg acctgcgctg
      241 ggcctaccgc gagaaactgg cgcgcctgca ggatcgcgag ctcacgctcg aaacgaagta
      301 ccccgtgctg tacgcgaacg tttcgcgcgc cgtgaaaggc ctgtggggcg gccagttgga
      361 cacgtggcgt gccagcgtcc tggtcgagaa gaacttccgc attcgcccgt tggggcgctt
      421 gagcgtgcag ttgctgggcg gcatggccga cccgcgcgca cccatgccct tcctcttcaa
      481 cctgcggggc acgtacagca cgcgcgatct gctgctggcc ggcgacaacg ccttcgagac
      541 catgcgcccc aacgaattcc tggcggaccg gtacgccacg ttgcacctga agtacgtgct
      601 caaatggccg ctataccggc tgaaatggtc gcgcccggtg cccgcgctcg tcaccggcct
      661 ggggttgggt gcgctggatc gaccgcagga tcatggcgga ctgcccttca gtcccatgtc
      721 cggcctctac accgaaagcg gtatccggct cgatggcctc ctcatcctca acggcctgat
      781 gcacttcggc gtgggtgcgt actaccgcta cggcgagcac gccctgccca cggccacgga
      841 caattgggtc ttcaaattga cggcggggct acggttacag tgacctgact ttccccgcac
      901 cgcatttcca ccgccaatgc gcaccttcgc ggcgaccttg caccagcggg gtcctgctgg
      961 atccgcatgt gggagtggtt ggcgagccgt gtactgcgca accgcacggc gatcctgatc
     1021 ggggtgctgg tggtcacggt tttcatgggc tacatggcca ccaccgtggg c
//
LOCUS       DAOQOC010001254         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139244_length_1071_cov_2.758858, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001254 DAOQOC010000000
VERSION     DAOQOC010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139244_length_1071_cov_2.758858"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PL106_15945"
     CDS             <1..264
                     /locus_tag="PL106_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34622.1"
                     /translation="MIFAVYGWGYLNGDWTEYAPDGHITLKGRYKNGKRDGPWAFREE
                     GIFGHYKEGLKSGKWKYYENGRVVRIEHFRKGVSGGGKRSLMK"
     gene            358..>1071
                     /locus_tag="PL106_15950"
     CDS             358..>1071
                     /locus_tag="PL106_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNA34623.1"
                     /translation="MTRSFVCGCALLTGWIGSLSNTEAQTNPATVGKLEALLYQIDQM
                     YVDSVDQNKLVETAITSMLEELDPHSIYIPKEELSEVNEPLKGNFEGVGIQFNLVRDT
                     IYVVDAIAGGPSERLGIRSGDRIIGIDGENVAGAGFKNKDVMNRLRGKKGTKVDVVIL
                     RTGSTDPLDFTITRDKIPIFSVEASYMATPTIGYIKVSRFSATTMKEFREKLSSLQKQ
                     GMKDLILDLQGNGGGYLRTA"
BASE COUNT          249 a          307 c          320 g          195 t
ORIGIN      
        1 atgatcttcg ccgtctatgg ctggggctac ctgaacggcg actggaccga atacgccccg
       61 gacggacaca tcaccttgaa gggccgctac aagaacggca agcgcgatgg tccttgggct
      121 ttccgcgagg agggcatctt cggccattac aaggaagggc tgaagagcgg caagtggaaa
      181 tactacgaga acggccgggt ggtgcgcatc gagcacttcc gcaagggcgt ctccggtggc
      241 ggcaagcgct cgctgatgaa atgagcgaac atgcgcgggg cgtcctgcgt taacacgctt
      301 ttggcagcgc tcccccgggc caacactact ttcacggcat ccagaactcc tttcctaatg
      361 accaggtctt tcgtttgtgg ttgtgcattg ctcaccggat ggatcggttc actgtccaat
      421 accgaagccc agaccaaccc cgctactgta ggcaaactgg aagcgctgct gtaccagatc
      481 gaccagatgt acgtggacag cgtggaccag aacaaactgg tggagaccgc catcaccagc
      541 atgctcgaag agttggatcc gcacagcatc tacatcccga aggaggagtt gtccgaggtg
      601 aatgaaccgc tcaagggcaa ctttgaaggc gtcggcatcc agttcaacct cgttcgcgac
      661 accatttacg tggtggatgc cattgctggc ggacccagcg agcgcctggg catccgcagc
      721 ggcgaccgaa tcatcggcat cgatggcgag aacgtggccg gtgcaggttt caagaacaag
      781 gacgtgatga accgcctgcg cggcaagaag gggaccaagg tggatgtggt catcctgcgc
      841 accggatcga ccgacccgtt ggatttcacc atcacacgcg acaagatccc cattttcagt
      901 gtggaagcca gctacatggc cacgcccacg atcgggtaca tcaaggtgag ccgtttcagt
      961 gccaccacca tgaaggaatt ccgcgaaaag ctcagttccc tgcagaagca aggcatgaag
     1021 gacctgatcc tcgacctgca gggcaatggc ggtggatacc tgcgtacggc g
//
LOCUS       DAOQOC010001255         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139254_length_1071_cov_2.696850, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001255 DAOQOC010000000
VERSION     DAOQOC010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139254_length_1071_cov_2.696850"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PL106_15955"
     CDS             <1..454
                     /locus_tag="PL106_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNA34624.1"
                     /translation="PTTGLDPNQIVEIRALIKQLGQAKTVIFSTHILGEVEAVCDRIL
                     IINHGRIVADSPPGALRTQSQGERIIFARIADGVRDEVLAALATLPQVAAVAPVNGTP
                     DAYELRGNAGADPAREVFHLCVARSWTLTELRPVETRLEDIFRDLTLN"
     gene            464..>1071
                     /locus_tag="PL106_15960"
     CDS             464..>1071
                     /locus_tag="PL106_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNA34625.1"
                     /translation="MRTVLLIARKELASFFDSLMAYLILVAFLGTSGFFTWWFGTDVF
                     MRGLTDLSTFFNVAYWTLFFFTPAITMRTIADERRTGTLDLLLTKAVSDRQVVVGKYL
                     ACVALIALALLCTLPYWITLAFIGKVDHGAVACGYLALLCMSSAYLALGVFASSFTNN
                     QIVAFLLGLSMVALFHMAFSTMASNFTGITGETLHQLGTQAH"
BASE COUNT          185 a          359 c          317 g          210 t
ORIGIN      
        1 gccgaccacc ggcctggacc cgaaccagat cgtggagatc cgcgcgctca tcaagcaact
       61 cgggcaggcc aagacggtga tcttcagcac gcacatcctg ggcgaagtgg aggccgtgtg
      121 cgaccgcatc ctgatcatca accatgggcg catcgtagcg gacagcccgc ccggtgcctt
      181 gcgcacgcag agccagggcg agcggatcat cttcgcgcgg atagccgatg gggtgcgcga
      241 tgaggtgctg gcggcacttg ccaccctgcc ccaagtggcc gctgtcgcac ccgtcaacgg
      301 cacccctgat gcgtatgaat tgcgcggcaa cgccggagcc gatcctgccc gagaggtatt
      361 ccacttgtgc gtggccagga gttggaccct taccgagttg cgccccgtgg aaacgcgact
      421 ggaggacatc ttccgggacc tcactttgaa ctgacggaag gagatgagga ccgtcctgct
      481 gatcgcccgc aaggaactcg cgtcgttctt cgactcgctg atggcctacc tcatcctggt
      541 ggcgttcctg ggcaccagcg gctttttcac ctggtggttc ggtaccgatg tgttcatgcg
      601 cggactgacc gatctgagca cattcttcaa tgtggcgtat tggacgctgt tcttcttcac
      661 ccccgccatc accatgcgta ccatcgccga cgaacgcagg acaggcacgc tggacctgct
      721 gctcaccaag gccgtgagcg accggcaggt ggtggtgggc aaatacctgg cctgcgtggc
      781 gttgatcgca ctcgccctgc tctgcaccct cccctactgg atcaccctcg cattcatcgg
      841 caaggtggac cacggtgccg tggcctgcgg ctacctggca ttgctctgca tgagttcggc
      901 gtacctcgcc ttgggcgtct tcgccagcag cttcaccaac aaccagatcg tggccttcct
      961 gctggggctg tccatggtgg ctctcttcca catggccttc tccaccatgg cctccaactt
     1021 cacgggcatc acgggcgaaa cgctgcacca gctcggcacg caggcccact t
//
LOCUS       DAOQOC010001256         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139353_length_1071_cov_2.023622, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001256 DAOQOC010000000
VERSION     DAOQOC010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139353_length_1071_cov_2.023622"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PL106_15965"
     CDS             complement(<1..328)
                     /locus_tag="PL106_15965"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNA34626.1"
                     /translation="MKIKTILVSQPEPTDEKNPYAELARKYNLKIEFRPFIKIEAVPG
                     QEFRQERINILDHSAIVLTSRNAVDHFFRMCKELRLTVPESMKYFCVSESVAYYVQKY
                     IVYRKRK"
     gene            complement(340..>1071)
                     /locus_tag="PL106_15970"
     CDS             complement(340..>1071)
                     /locus_tag="PL106_15970"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNA34627.1"
                     /translation="VHNGVGDGPVVSRTMEEVLRAADPLRAGWVAALVLALVAMLGWI
                     NLVSPKKWRLLGDAFRSLRLGRQTMRDDVDLQDRTLVVLLVMATASLGLFAHQFSVLL
                     LGAAADAGHWAKCFGLVAGVLVALFLVNRATALLARADAGLTEHLYNTLLLLIVLGVA
                     LLPLLAVVAWPHQPAWRMPALWIALGLAALLMVWRWVRAAAIGLAEGVPLRYIFIYLC
                     ALEILPVVILGQRLIRSVALASPTP"
BASE COUNT          211 a          358 c          348 g          154 t
ORIGIN      
        1 ccttgcgctt gcggtacacg atgtacttct gcacgtagta ggccacgctc tcgctaacgc
       61 agaaatactt catcgactcc ggcaccgtca ggcggagctc cttgcacatg cggaagaaat
      121 ggtccaccgc attgcggctg gtgagcacga tggccgagtg gtccaggatg ttgatgcgct
      181 cctgccggaa ctcctggccg ggcaccgcct cgatcttgat gaacggacgg aactcgatct
      241 tcaggttgta tttccgggcc agttcagcgt acggattctt ctcgtccgtc ggctccggct
      301 gggaaacgag gatcgtcttg atcttcaagg ccgctcggct taaggggtgg gggaagcaag
      361 cgcaacgctg cggatcaagc gctggcccag gatgacaact ggcagaattt caagggcgca
      421 aaggtagatg aaaatgtagc gcaaggggac cccctcggcc agtccgatgg ccgccgcccg
      481 cacccagcgc cacaccatga ggagggccgc caggccgagg gcgatccaca gggcgggcat
      541 gcgccaggcg ggctgatgcg gccaggccac cacggccagc aggggcagca gggcgacgcc
      601 cagcacgatg agcagcagca gcgtattgta caggtgctcg gtgagcccgg catccgccct
      661 ggccagcaag gcggtggcgc ggttgaccag gaagagggcg accaacacgc cggccacgag
      721 gccgaagcat ttggcccaat gccccgcatc ggccgccgca cccagcagca gcacgctgaa
      781 ctggtgtgcg aagagcccga ggctggccgt ggccatcacc agcagcacca ccagggtgcg
      841 gtcctgcagg tccacatcgt cgcgcatggt ctggcggccc aggcgcaggg aacggaacgc
      901 atcgcccagc aggcgccact tcttgggcga caccaggttg atccagccca gcatggccac
      961 caaggccagc accagcgcgg ccacccaccc cgcccgcagg ggatccgcgg ccctgagcac
     1021 ctcctccatg gtgcgcgaaa ctaccgggcc atcgcccacc ccgttgtgca c
//
LOCUS       DAOQOC010001257         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139411_length_1071_cov_1.593504, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001257 DAOQOC010000000
VERSION     DAOQOC010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139411_length_1071_cov_1.593504"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PL106_15975"
     CDS             complement(<1..302)
                     /locus_tag="PL106_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34628.1"
                     /translation="MTLPKKYEPCLCEVQYLHERRWEVLYHHARKGWVFADGLGLTTT
                     ASVIQWMAIADITPSEARWPVWNTGRFAAHWLLWKRHKQERRESYKPTGERIALMQ"
     gene            complement(299..793)
                     /locus_tag="PL106_15980"
     CDS             complement(299..793)
                     /locus_tag="PL106_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34629.1"
                     /translation="MIQELKRRIHDDIAWNQERSQTSVYDRERAYHAEQVQKLLLVLE
                     TLDTIPEPTPPGPSPEKPMSLKEMIGYMGKRPGITTPMLMPRMPTKEEAALALACHLM
                     DTGSGDWDEETVMRRKEWRKELEKVREREFRIATSIKATMHLKVREYHKMYGVSIGNH
                     NRVV"
     gene            complement(790..1041)
                     /locus_tag="PL106_15985"
     CDS             complement(790..1041)
                     /locus_tag="PL106_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34630.1"
                     /translation="MKAKIEATLSPSNTISAEVGDIFWVKGQMGRYLVIVTITENGYQ
                     LTSLDDGARWSGECVDLDEVNGQLLASEARPANVKIVNA"
BASE COUNT          184 a          334 c          283 g          270 t
ORIGIN      
        1 atctgcatca gggcgatgcg ctccccggtg ggtttgtagc tctcgcgacg ctcctgctta
       61 tggcgtttcc acagcagcca gtgcgcagcg aatcgtcctg tattccacac cggccatcgc
      121 gcttcacttg gagtgatgtc ggcaatggcc atccactgga tcaccgaggc ggtggtggtc
      181 agccccagcc catcggcgaa cacccatccc tttctggcgt ggtggtagag aacctcccat
      241 cggcgctcgt gtaggtactg tacctcgcac aagcatggct cgtatttctt ggggagggtc
      301 atactactct gttgtggtta ccgatcgaca ctccgtacat cttgtgatac tcccgcacct
      361 tcaggtgcat ggtcgccttg atcgaggtgg cgatgcggaa ctcccgttcc ctcaccttct
      421 ccaactcctt ccgccactcc ttccggcgca ttaccgtttc ttcatcccaa tcccccgacc
      481 ctgtatccat cagatgacag gccagcgcca gtgccgcctc ttccttggtc ggcatccgtg
      541 gcatcagcat cggcgtggtg atgccaggac gtttccccat gtatcctatc atctccttga
      601 gcgacatggg tttctccggc gatgggccgg gaggggtggg ttcggggatg gtgtcgagcg
      661 tctccagcac aaggagcagc ttctgcacct gctcggcatg ataggctctc tctctgtcat
      721 acacggaggt ctgggaccgc tcttgattcc acgcgatgtc gtcgtggata cggcgcttga
      781 gttcctgtat catgcgttga cgatcttgac gttggcgggc cgggcctcgg aggcaagaag
      841 ctgtccattc acctcatcaa ggtcaacgca ctcaccggac cacctcgctc catcgtcaag
      901 cgacgtaagc tgatagccgt tctctgttat tgtcacgatc accagatacc tgcccatctg
      961 gcccttgacc cagaaaatat cgcctacctc agcagatatg gtgttgctcg gtgacagtgt
     1021 tgcttcgatc tttgctttca tcgcttgctc cattcaaggg gttcaatgat c
//
LOCUS       DAOQOC010001258         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139617_length_1070_cov_2.156650, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001258 DAOQOC010000000
VERSION     DAOQOC010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139617_length_1070_cov_2.156650"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PL106_15990"
     CDS             complement(<1..521)
                     /locus_tag="PL106_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNA34631.1"
                     /translation="MSCCTRWLLCAVTGFLWSHCAAQWTFTGNTTPTWQEAIARYAEL
                     DSLHTGAQLQVMGYDDNGLPIHLFVVSDGSGFSPDSIRASGKNILWITNGIHPGEPDG
                     VDASLLLARALLESDQLMGLTVHTAVCIVPMYNVSGALQRNGTLRVNQNGPEAYGFRG
                     NARNLDLNRDFMK"
     gene            789..>1070
                     /locus_tag="PL106_15995"
     CDS             789..>1070
                     /locus_tag="PL106_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34632.1"
                     /translation="MKKLLMLMVLAAMTATVQAQDTGKRTTQERAHARSERMAKELGL
                     SPEQQAKVEAIDLKYAERSQALRAEREAKEDGARKDGQGRAIRDAREVEM"
BASE COUNT          225 a          307 c          348 g          190 t
ORIGIN      
        1 attttcatga agtcacgatt gaggtccagg ttgcgtgcat tgcctcggaa gccatacgcc
       61 tcggggccgt tctggttcac ccgcagggtg ccgttgcgtt gcagcgcacc gctcacgttg
      121 tacatgggta cgatgcacac ggcggtgtgg acggtgagcc ccatcagttg gtcgctctcc
      181 agcagcgccc gtgccagcaa gaggctcgca tccacgccgt cgggttcgcc gggatggatg
      241 ccgttggtga tccacaggat gttcttcccg cttgcacgga tgctgtcggg ggagaagccg
      301 ctgccatcgc tcaccacgaa gaggtggatg gggagtccgt tgtcgtcgta ccccatcacc
      361 tgcaattggg cgccggtgtg caggctgtcg agctctgcgt agcgcgcgat ggcctcttgc
      421 caggtgggtg tggtgtttcc agtgaaggtc cattgagcgg cacaatgcga ccagaggaaa
      481 ccagtgacgg cacacaggag ccatcgtgtg caacaggaca tcgggtcagg catatgtgaa
      541 tttcggtatg atgatccgaa gcacctccat cgctcggccc ggaggaaggg gggcctcgat
      601 gcggaactgc gacagcgtca ccaatgtcgg tccccgggtg ttccgcgttc ccatcacggc
      661 aaaggaacag cgggacaacc tcaggacatc atcggaccgg ctcctcaatg atcgttaacg
      721 gatcgaggtc cgttgaacct tgtggcccga tgcttgtcac aacaccaccg aacccaacgt
      781 gaacgaacat gaagaagcta ttgatgctga tggtgctggc cgctatgacc gccaccgtcc
      841 aagcccagga caccggcaag aggaccactc aggaaagggc tcatgcgcgc tcggaacgca
      901 tggccaagga actcggcctg agtccggagc aacaagcgaa agtggaagcc atcgacctga
      961 agtacgccga gcggtcgcaa gccttgcgtg ccgaacgcga agccaaggag gatggggcac
     1021 ggaaggatgg ccaaggccgc gcgatccgcg atgcgcgcga ggtggaaatg
//
LOCUS       DAOQOC010001259         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139802_length_1069_cov_3.071992, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001259 DAOQOC010000000
VERSION     DAOQOC010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139802_length_1069_cov_3.071992"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PL106_16000"
     CDS             complement(<1..879)
                     /locus_tag="PL106_16000"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNA34633.1"
                     /translation="MLKRLWRKLLYSRANRSLISWAQRIRFPGSGGFSVYHIARFFFL
                     ALAEGRLTTRASAIAFKLFLAFFPIIILLLTLIPYIPVADFQEKLLHNFQAMLPPAVF
                     DFIHGLLHDLVVRKHGGLLSVSFITGLYFASNGLDAILMGFRGSYHIERWHSPLMQRL
                     LSLGLLLALTVMMVVAMALLTFSNHVIRMLPDLGIRLGDLEHFGLFTAKWSITVVLIT
                     ACIGLLYSAGDPGARRFRIFTPGAVLAVILTLLLSQALAFFFARITDYNALYGSLGAI
                     LAVQLWLYFNMLVLLIG"
     gene            complement(886..>1069)
                     /locus_tag="PL106_16005"
     CDS             complement(886..>1069)
                     /locus_tag="PL106_16005"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363851.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNA34634.1"
                     /translation="GLDDLRTAVDMIDRACETEASGGITLATARAVADTGVEFLSVGA
                     LTHSAGSLDLSLKAMN"
BASE COUNT          247 a          307 c          349 g          166 t
ORIGIN      
        1 gccgatcaac aggaccagca tgttgaaata cagccagagt tgcacggcaa gaatggcgcc
       61 gagcgatccg tacaacgcgt tgtaatcggt gatacgggcg aaaaagaagg ccaacgcctg
      121 ggacagcaac aacgtgagga tcaccgcgag gaccgcacct ggggtgaaga tccggaatcg
      181 gcgtgctccg gggtcgcccg cgctgtacaa caggccgatg catgcggtga tcagcaccac
      241 cgtgatgctc cacttcgccg tgaagagacc gaaatgctcc agatccccca agcggatacc
      301 cagatcgggc agcatgcgga tcacgtggtt gctgaaggtg agcagggcca tggccaccac
      361 catcatcacc gtgagggcca gcagcagtcc caggctcaac agccgttgca tgaggggcga
      421 atgccagcgc tcgatgtggt aactgccgcg gaaacccatc aggatcgcgt ccagtccgtt
      481 gctggcgaag tacaggccgg tgatgaagct cacgctgagc aacccgccgt gcttgcgtac
      541 caccaggtcg tgcaacagac catggatgaa atcgaacacc gccggtggga gcatcgcctg
      601 gaagttgtgc aagagcttct cctggaagtc ggccacgggt atgtacggga taagcgtgag
      661 cagcaggatg atgatcggaa aaaaggccag gaagagcttg aaggcgatgg ccgatgcgcg
      721 tgtggtgaga cggccttcgg caagggccag gaagaagaaa cgggcgatgt ggtacacgct
      781 gaagccaccc gagcccggga accggatgcg ctgcgcccac gaaatgaggc tgcggttggc
      841 gcgcgagtac aggagctttc gccagagccg tttcagcatg agggttcagt tcatggcctt
      901 cagactcagg tccaggctgc ccgcactgtg cgtgagcgcg cctacactga ggaactcaac
      961 gccggtatcg gccacagcac gcgccgtggc cagggtgatg cctccgctgg cctcggtctc
     1021 gcaggcccgg tcgatcatat caacggccgt acgaagatcg tccagtccg
//
LOCUS       DAOQOC010001260         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139805_length_1069_cov_2.962525, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001260 DAOQOC010000000
VERSION     DAOQOC010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139805_length_1069_cov_2.962525"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PL106_16010"
     CDS             complement(<1..196)
                     /locus_tag="PL106_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA34635.1"
                     /translation="MSVQMTFRNLLALPVFLALAGLRAQAPVRPLVEVNTTQGRMVWA
                     LYNETPLHRDNFLRLVQEHYY"
     gene            276..665
                     /locus_tag="PL106_16015"
     CDS             276..665
                     /locus_tag="PL106_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34636.1"
                     /translation="MIRKVFLFALAAVILVPFTSSCNKEKDTLAIITVLDTAGHPVAG
                     AYVKLFANLAHQPQGAGSMDRLTREEMTDSRGHARFDYTQQYKQGQAGFAVLDIVSFK
                     DSAVGEGIIKILEEEVNEETVKLTPVN"
     gene            complement(728..>1069)
                     /locus_tag="PL106_16020"
     CDS             complement(728..>1069)
                     /locus_tag="PL106_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34637.1"
                     /translation="QFDKRWRDLMKRMETRFQGPVDLNGLIFLIGVQELGQHARDFKK
                     DEKVQLMHIGICVVLLPFGYYAELGRDADGWPHFERVKDLPPLNAQEQERLMKEAVLN
                     YFGEAQVTTHA"
BASE COUNT          198 a          318 c          345 g          208 t
ORIGIN      
        1 cgtagtaatg ctcctgtacc agcctcagga agttgtcgcg gtgaaggggc gtttcgttgt
       61 acagcgccca caccatgcgg ccctgggtgg tgttcacctc caccagcggg cgcaccggag
      121 cctgggcccg aagcccggcc agggccagga agacaggcaa cgccaggagg ttccggaagg
      181 tcatctgtac ggacatggcc cgatgctgct atttttggct gcctcttacc aaggtccgcc
      241 gcgttgagcg gccgaaatta acccgaccaa cccccatgat ccgtaaggtg ttcttgttcg
      301 cgctggccgc ggtcatcctg gtgccgttca cctcgtcctg caacaaggag aaggatacgc
      361 tggccatcat taccgttctg gatacggccg ggcatcccgt ggccggggcc tatgtgaagc
      421 ttttcgccaa cctggcccac caaccgcagg gggcgggatc catggaccgc ctgacgcgtg
      481 aggaaatgac cgattcgcga ggtcatgccc gtttcgacta cacccagcag tacaagcagg
      541 ggcaggcggg gttcgcggtg ctcgatatcg tgagcttcaa ggacagcgcg gtgggcgagg
      601 ggatcatcaa gatcctggaa gaggaggtga acgaggagac cgtgaagttg accccggtga
      661 actgagcggg ccggagggcg cccaaggagt ggtgtctgcc gggtgaaggc gcgcggcccc
      721 caagggttca ggcgtgcgtg gtcacctgtg cttcgccgaa gtagttcagc acggcctcct
      781 tcatcaggcg ttcctgttcc tgggcattca gtggcggcag gtctttcaca cgttcgaaat
      841 gcggccatcc gtcggcatcg cggccgagtt ccgcatagta gccgaagggc agcaggacca
      901 cgcagatgcc gatgtgcatc agctgcacct tctcgtcttt tttgaaatcg cgggcgtgct
      961 ggccgagttc ctgcaccccg atcaggaaaa tgagtccgtt caggtccacc ggtccctgga
     1021 agcgggtttc catccgcttc atcagatcgc gccagcgctt gtcgaattg
//
LOCUS       DAOQOC010001261         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139885_length_1069_cov_2.266272, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001261 DAOQOC010000000
VERSION     DAOQOC010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139885_length_1069_cov_2.266272"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..>1069)
                     /locus_tag="PL106_16025"
     CDS             complement(95..>1069)
                     /locus_tag="PL106_16025"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNA34638.1"
                     /translation="VVAGRGTFAIGISALTFLKIDPSPRSAAMASANVSTMGDAYSTF
                     TNPAGLADVDKFSIAASNTFWPAGINYAFFSAIKPTRVGFFGLSAASLTTGKMERRTE
                     FQPDGTGQYFSASNTAIGLTYSQRLTDYFSYGVTLKYVNEQLAEYMAHTGVVDLGFLY
                     RTDWKDLSFAVAMMAFGTNSKLNGSYQPDLLNSKPVTLGSYPAPTLFKLGVSMVPWKR
                     DDMSLTTIVQLNHPNDNAENIRIGLEYEYKKLLYLRAGYKINVDDEVYPTAGVGIRAH
                     IGKHPLHIDYAVDPTRYLGWIHRVGLSFGFNKLDREKKNEEAAPAPAN"
BASE COUNT          204 a          298 c          343 g          224 t
ORIGIN      
        1 gctcgggtcc agttttttcc cgaagaagcc ttcacatcca gcgctcagca ggacgagggc
       61 ggccaccatc aacagggagc caaggcccag gcgatcagtt ggcaggtgca ggtgctgctt
      121 cttcattttt cttctcccga tccagtttat tgaagccaaa ggaaaggccg acccggtgta
      181 tccagcccaa gtagcgggtc ggatccactg cgtagtcgat gtgcaaggga tgcttgccga
      241 tatgcgcccg gatgcctacc ccagcggtgg gatagacctc gtcgtccaca ttgatcttgt
      301 agccggcccg caggtagagc agcttcttgt attcgtactc caacccgatg cggatgttct
      361 cagcattgtc attggggtgg ttcagctgca cgatggtggt gaggctcatg tcatcacgct
      421 tccacggcac catcgacaca cccagtttga agagggtggg cgcaggatag ctgcccaagg
      481 tcacaggctt gctgttcagc aggtcgggtt ggtagctgcc attgagcttg gagttcgtgc
      541 caaatgccat catggccaca gcaaagctca ggtccttcca gtccgtccgg tacaggaagc
      601 ccaggtccac gactccggta tgggccatgt actctgcgag ctgctcgttc acgtatttca
      661 gtgtgacgcc gtagctgaag tagtcggtca gccgctgcga ataggtcagg ccgatcgcgg
      721 tattgctggc actgaagtat tggccggtgc cgtcgggctg gaactcggtg cggcgctcca
      781 tcttgcccgt ggtgagcgag gcagcgctca ggccgaagaa gccaacgcgg gtaggcttga
      841 tcgcggagaa gaaggcgtaa ttgatcccgg cgggccagaa ggtgttcgag gcagcgatgc
      901 tgaacttgtc cacgtcggcc agtcctgccg ggttggtgaa ggtgctgtag gcatcgccca
      961 tggtggacac gttcgcactg gccatggcgg cggagcgcgg gctgggatcg atcttcagga
     1021 aggtcagcgc ggagatccct atggcaaagg tgcctcgccc ggccaccac
//
LOCUS       DAOQOC010001262         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_139914_length_1069_cov_2.052268, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001262 DAOQOC010000000
VERSION     DAOQOC010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139914_length_1069_cov_2.052268"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="PL106_16030"
     CDS             complement(<1..>1069)
                     /locus_tag="PL106_16030"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA34639.1"
                     /translation="QVDQKAISAYCVDKIELAARGKIDPIVGRDREVRMMTEILGRRS
                     KPNVIIVGEPGVGKTALVEGFALNIQRGDVPERLKGSRLFELDLGSLVAGASYKGEVE
                     DRIKKVMNAIKAHDKAILFIDEIHALMDDARGLSGCADLLKPELARGELTVIGATTRD
                     EHRTFLEPESAFMRRFELITVDEPNDRVCKQMLRTVVPIYEKHHALQVDEKALEESIR
                     LSRRYLKERRLPDAAIDLIDRTLASIRMMNDTSEGDITRFRTRLDEVLAEDEALQLED
                     LRHLPVLEKRDIQERGAEMVAEGWPEDDLIRNQTGGSTGAPIRFFLCRDRKCSRAAAT
                     LRHNRWAGWREGDSAAVIWGAP"
BASE COUNT          159 a          361 c          333 g          216 t
ORIGIN      
        1 ggggcgcccc agatcaccgc cgccgagtcg ccctcccgcc acccggccca ccggttgtgg
       61 cgcagggtgg ctgccgcccg tgaacacttc cggtcgcggc agaggaagaa gcggatcggg
      121 gcgcccgtcg agccgcccgt ctggttgcgg atcaggtcgt cttcgggcca gccctccgcc
      181 accatctccg ccccgcgctc ctggatgtcg cgcttctcca ggaccggcag gtgccgcagg
      241 tcctccagct gcaacgcctc gtcctcggcc agcacctcat ccagccgtgt ccggaagcgc
      301 gtgatgtccc cctcggaggt atcgttcatc atgcggatgg aggccagggt acggtcgatc
      361 aggtcaatgg ccgcatcggg cagcctgcgt tctttcaggt agcggcgcga gagccggatg
      421 gattcctcca gggccttctc gtccacttgc aaggcgtggt gcttctcgta aatgggtacc
      481 acggtccgca gcatctgctt gcacacgcgg tcgttgggct cgtcgacggt gatcaactcg
      541 aagcggcgca tgaaggcgct ctcgggttcc aggaaggtgc gatgctcatc gcgtgtggtg
      601 gccccgatca ccgtgagttc accacgagca agctcgggct tcaacaggtc ggcacagccg
      661 ctcagtcccc gggcatcgtc cataagtgca tggatctcgt caatgaacag gatggccttg
      721 tcgtgggcct tgatggcgtt catcactttt ttgatccggt cctccacttc gcccttgtag
      781 ctggctccgg caaccagcga gcccaggtcc agttcgaaca ggcgcgagcc tttcaaccgc
      841 tcgggtacgt cgccccgctg gatgttcagg gcgaagcctt ccaccagggc cgttttgccc
      901 acgccgggct cgcccacgat gatcacgttg ggtttgctgc gccggcccag gatctcggtc
      961 atcatgcgca cttcgcggtc gcgcccaacg atggggtcga tctttcctcg tgcagcgagt
     1021 tcgatcttgt ccacgcagta ggcgctgatg gccttctggt ccacttgac
//
LOCUS       DAOQOC010001263         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_140012_length_1068_cov_4.451135, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001263 DAOQOC010000000
VERSION     DAOQOC010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140012_length_1068_cov_4.451135"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="PL106_16035"
     CDS             complement(<1..102)
                     /locus_tag="PL106_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA34640.1"
                     /translation="MALEFTDSNFEELVLKSDKPVLVDFWAEWCGPCR"
     gene            complement(648..977)
                     /locus_tag="PL106_16040"
     CDS             complement(648..977)
                     /locus_tag="PL106_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34641.1"
                     /translation="MSFEVHTIRPFERQLKRLVKAYPSLRQEYALLIRSLEEQPDQGT
                     PLGAGCYKVRVSIASKGKGKSGGARVITHLAVRERRVWLLSIYDKSEVDSVPLAYVKA
                     LLGHIPP"
BASE COUNT          195 a          307 c          304 g          262 t
ORIGIN      
        1 gcggcagggg ccgcaccatt ccgcccagaa gtccaccagg acgggcttgt cgcttttaag
       61 gaccagttct tcgaagttgc tgtcggtgaa ttcgagtgcc atgattctgg ccgaccttgg
      121 aatgcgggag tcggcacccg ctcttggttt atgatcgcgt ccttgctggt tgcaaagttg
      181 gccttatcgg gctgcgaagt cccttgaagt ggttcaactt ttcaaccgga acttggacgg
      241 ctttcgcgcc acgatgggca aggtccgggc caggggaggg gaactgacag ttgggcagtt
      301 atggacgctt ctaagggatc atgttccgaa gggccaccaa ttcgtcgcgt gagatgctct
      361 tctgctcgga cttgtcgtac atggtcaata caggagtagc tcaccccagc ttcacctcca
      421 ccacctgtcc tccccaagcg ggatccgccg atcccccggg tggtccatca atccttcggt
      481 cccagccacc ttggcgccct tcgcgttcag ttccacgttc acgttctacg ctgcgctgaa
      541 aagcggaacg ttccacttgc acttgccggg gtattctttg aggagcgaag cgaacgcgcg
      601 ccccaggtcc tcgtgtacgt gctcggttgc gatcggaagg cgtggcttca ggggggaata
      661 tggcctaaca gtgccttcac ataggcgaga ggtacgctat ccacttcgga cttgtcatag
      721 atgctcagca accataccct acgttcccgg acagcaaggt gggtgatcac tcgcgcgccg
      781 ccgcttttgc cttttccttt tgatgcgatg gacacacgga ccttgtaaca tccggctcca
      841 agcggggttc cttggtcggg ttgttcttcg agagatcgga tcagcaaggc gtattcctgg
      901 cgtagagaag ggtatgcctt gaccagccgc ttcaactgcc gttcgaatgg cctgatggtg
      961 tggacttcaa agctcattca acagctcctt tgcaggtctg ccgcgctttt ttccagactt
     1021 gatgagtttc atttcctcca cggcctcctt gaggtcggca aggaacct
//
LOCUS       DAOQOC010001264         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_140023_length_1068_cov_4.035538, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001264 DAOQOC010000000
VERSION     DAOQOC010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140023_length_1068_cov_4.035538"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="PL106_16045"
     CDS             complement(<1..>1068)
                     /locus_tag="PL106_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNA34642.1"
                     /translation="AFACCSSIGIRAQQPGITRYDLDDGLPQSMVNHVLQDRDGFIWL
                     GTGDGLARFDGLRMLVYEHDPRDSSSLSHNAIWGLAEADEEHLWVATRTGLDLLDRRS
                     GTFEHVATGAGVNGCWIPVKSTGDEAIFYSPLSRDLLRINSGRTSRIPSGHLDSYAFD
                     VNKEGDLCLYTTDSIIVLPLDDRPGTAIKLDRTGDVSAIIEVEGKWLLLTDRGGWLVH
                     RDGTHEDPPAELRNYLQQSRGLKYAARDPHGRLWIAISNVGVVQLRDDLSIERYYSLM
                     DANEGSLSVTRIAFDRQGNAWVGTDGKGVIKIAPQRIKFQRCMAGMGLSWEPPSWFVR
                     GFAQWDAQRVLVAFHQGGLAL"
BASE COUNT          222 a          343 c          307 g          196 t
ORIGIN      
        1 aaaagagcca ggccaccttg gtggaaggcc accagcaccc gctgcgcatc ccattgcgcg
       61 aatccacgca cgaaccagga ggggggctcc caggacagtc ccatgcctgc catgcaccgc
      121 tggaacttga tgcgttgcgg tgcgatcttg atcactcctt tcccatcggt acccacccag
      181 gcattgccct gccgatcaaa agcgatccgg gtgaccgaca atgagccttc gttggcatcc
      241 atcaacgaat aataccgttc gatggagagg tcatcacgca gctgcacgac accgacgttg
      301 ctgatggcga tccacaaccg gccatgtgga tcgcgggcgg catacttcag tcccctggac
      361 tgttgcaggt agttcctcag ttccgctggc ggatcctcgt gcgtgccatc ccgatggaca
      421 agccagccgc cacggtcggt gaggagcagc cacttccctt ccacctcgat gatcgcggac
      481 acgtcacccg tccgatcgag cttgatggcg gtccccggcc tgtcgtccag cggcagaacg
      541 atgatcgaat ccgtcgtata gaggcagagg tcgccttcct tgttcacatc gaatgcatac
      601 gaatcgagat gcccgctggg gatacgtgat gtccgcccgc tattaatgcg caagagatcg
      661 cgtgaaagag gtgaatagaa gatcgcttca tcaccggtgc tcttgaccgg gatccagcat
      721 ccattcacac cggcgccggt ggcgacgtgc tcgaacgtgc cggatcggcg atccagcagg
      781 tcaagccccg tgcgtgtggc cacccagagg tgttcctcat cggcttcggc cagaccccaa
      841 atggcgttgt gcgaaagact ggaactgtcg cgcggatcat gctcgtacac caacatgcgc
      901 aggccgtcga accgcgccag tccatcaccc gtaccgagcc agatgaaccc atcacggtcc
      961 tgcagcacat ggttcaccat gctctgcggc aggccgtcgt ccaggtcgta acgggtgata
     1021 ccgggttgct gggcccggat cccgatcgag gaacagcagg cgaaagcc
//
LOCUS       DAOQOC010001265         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_140810_length_1066_cov_1.306627, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001265 DAOQOC010000000
VERSION     DAOQOC010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140810_length_1066_cov_1.306627"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PL106_16050"
     CDS             <1..558
                     /locus_tag="PL106_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34643.1"
                     /translation="GMAVRIDLTRAISLETGISQITRRFDVAFSNDTNGYAVSDKVRL
                     VGYEIPVLAMVYIRLGRDTWMNNALGVSADMYPSDAVRTQDFSRVYMARKNWISPGIV
                     ANIGFEYRSPKSGWFYLGATYHRPFGNIARAEYTWYDRSAFGTTVFAGIAGSYLTADL
                     RYYFHNDPDKSRRRKADTRNRGGDQ"
     gene            639..>1066
                     /locus_tag="PL106_16055"
     CDS             639..>1066
                     /locus_tag="PL106_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34644.1"
                     /translation="MNRLYTPFLLLPFLPAIVRAQGDDCASALAVVPGAYSADGPSAG
                     GGAGQDGALNADWYQWTASEQVYITVQSCGSGVDTRLHVYTGACGELELVASDDDGCP
                     EGASGGSLVANLLVDAGATYYFEWDDAHSTAGFDWSFQPHA"
BASE COUNT          200 a          333 c          323 g          210 t
ORIGIN      
        1 ggcatggccg tccgcatcga cctcaccagg gccatctccc ttgaaacagg tatcagtcag
       61 atcacccgcc ggttcgatgt ggccttctcc aacgacacca atggatatgc cgtcagcgac
      121 aaggtccgac tggtgggcta cgagatcccc gtgctcgcca tggtgtacat ccgcctcggt
      181 cgggacacgt ggatgaacaa cgcgctcggc gtgagcgccg acatgtatcc gagtgatgcc
      241 gtgcgcacgc aggatttttc gagggtgtac atggcccgga agaactggat aagccccggt
      301 atcgtggcca acatcggttt cgagtaccgc tcccccaaga gcggatggtt ctacctgggg
      361 gccacctacc accgtccctt cggcaacatc gcacgtgccg agtacacgtg gtacgaccgg
      421 tcagcgttcg gcacgaccgt tttcgcggga attgccggca gttacctcac cgcggatctg
      481 cgctactact tccacaacga cccggacaag tcccgacgca ggaaagccga tacacgcaac
      541 aggggcggcg accaataggc cgtacgcgac cccgttcgtg ttcggtgggc tgttccgatc
      601 acccggcccg gatatattcg gagcccatcc catcttcaat gaaccgcctg tacacaccat
      661 tcctcctgct cccgttcctt cccgccatcg tccgtgcgca aggcgatgat tgtgcttccg
      721 cattggccgt ggtcccgggc gcatacagcg cggatggacc ttcggccgga ggtggtgccg
      781 gccaggacgg tgcgctcaac gccgattggt atcaatggac ggcttcggaa caggtgtaca
      841 tcaccgtgca aagttgcggg tcaggggttg atacacgctt gcacgtgtac acgggggcct
      901 gcggcgaact tgaactggtg gccagcgacg atgatggctg cccggagggt gcatcaggtg
      961 ggagccttgt ggccaacctg cttgtggatg cgggtgccac gtattacttc gagtgggacg
     1021 atgcgcactc cacagcgggc tttgattggt ccttccaacc gcatgc
//
LOCUS       DAOQOC010001266         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_140859_length_1065_cov_3.399010, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001266 DAOQOC010000000
VERSION     DAOQOC010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140859_length_1065_cov_3.399010"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PL106_16060"
     CDS             complement(<1..291)
                     /locus_tag="PL106_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNA34645.1"
                     /translation="MSHSGPQDTIDLKAIFARMVGKWWLFAISIAIALAAGVAYIKTT
                     PKSYRVKAVLRMSEGRRSSFGGQKEEFLRGASYLKTDAELEDEITMIRSVSNM"
     gene            complement(291..>1065)
                     /locus_tag="PL106_16065"
     CDS             complement(291..>1065)
                     /locus_tag="PL106_16065"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNA34646.1"
                     /translation="MKFITPFLAIVVVATLSSCVGRRSVNYLQDPALSNTAKLFPNSR
                     FQYRVQVNDVLSIRVLGLDDATGRLFNIDQNNNNALSDAAIYVNGFSVDKTGHVQLPS
                     VGKLKVQGLTLSEAEELVQRRINDIFTNATVILKLVSFRVSVLGSVNAPGSYLIYNNQ
                     ITLLDALARAGGPQEYADKHHVTLMRQSDQGTQAVYVDLSRADVASSEYYYLLPNDVV
                     YVPSLKARPQRMNLELLGILFSAISAAAIVVTVVQNSNK"
BASE COUNT          187 a          322 c          325 g          231 t
ORIGIN      
        1 catgttgctc accgaacgga tcatggtgat ctcatcctcc agctccgcat cggtcttcag
       61 gtagctcgca ccgcgcaaga attcctcctt ctgcccgccg aagctcgacc tccgcccttc
      121 gctcatgcgc agcacggcct tcacacggta cgacttgggc gtggtcttga tgtacgccac
      181 gcccgccgcc agggcaatgg cgatgctgat ggcgaacagc caccacttgc cgaccatccg
      241 tgcgaagatc gccttcagat cgatggtatc ctgcggtccg gaatggctca ttacttgttc
      301 gagttctgca ccacagtgac cacgatcgcc gcggcgctga tggccgagaa gaggatgccg
      361 agaagctcca ggttcatgcg ctgcggtcgt gctttcaggc tgggcacgta taccacgtcg
      421 ttgggcagga ggtagtaata ttcgctgctg gcgacgtccg cacggctcag gtcgacgtac
      481 accgcctggg tgccctggtc gctctggcgc atcagggtaa cgtggtgctt gtcggcgtac
      541 tcctgcggcc cacccgcccg cgccagtgcg tccaatagcg tgatctggtt gttgtagatg
      601 aggtagctgc cgggtgcgtt caccgagccg agcacgctca cgcggaaact caccagtttg
      661 aggatcacgg tggcgttggt gaagatgtcg ttgatcctgc gctgcaccag ttcctcggct
      721 tcactcaagg tgaggccctg caccttgagc ttgcccacac tgggcagttg cacatgcccg
      781 gtcttgtcca cgctgaaccc gttgacgtat atggccgcgt cgctcaaggc gttgttgttg
      841 ttctggtcga tgttgaacag ccgtccggtg gcgtcgtcca gccccagtac acggatgctg
      901 agtacatcgt tcacctggac acggtattgg aaccgtgagt tggggaacag cttggccgtg
      961 ttgctcaatg ccggatcctg caggtagttg acgctgcggc ggcccacaca ggaactcagg
     1021 gtggccacga cgacaatggc caggaacggg gtgatgaact tcatg
//
LOCUS       DAOQOC010001267         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_141187_length_1064_cov_2.947473, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001267 DAOQOC010000000
VERSION     DAOQOC010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141187_length_1064_cov_2.947473"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PL106_16070"
     CDS             <1..188
                     /locus_tag="PL106_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34647.1"
                     /translation="VQDTGLEDLCHVHDDPGRMRLSIQACMAQHADGALVEKRMAVLE
                     QRFCNRKNAAAILKAMF"
     gene            complement(305..997)
                     /locus_tag="PL106_16075"
     CDS             complement(305..997)
                     /locus_tag="PL106_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA34648.1"
                     /translation="MTTDGPIIELNAARIFQRENLILDDVSFSVRKGEFIYLIGRTGS
                     GKSSLMRTLYGDLPLTQGEGHVCGYDLRKLKRREVPYLRRKLGIVFQDFQLLSDRNAN
                     ANLVFVMESTGWKDRKEIDARVKEVLEQVGLGNKGYKMPHELSGGEQQRVSIARALVN
                     RPELILADEPTGNLDPETTGEILDLLYTISQFGSSVIMATHDYTHMKKLNARVVRCEG
                     GKLLEKTAEVAG"
BASE COUNT          179 a          338 c          328 g          219 t
ORIGIN      
        1 tggtgcagga cacgggactg gaggacctgt gccatgttca tgacgacccc gggcgcatgc
       61 gactgagcat acaggcctgc atggcccagc atgccgatgg cgccctggtg gagaagcgca
      121 tggccgtgct ggagcagcgg ttctgcaaca ggaagaacgc tgctgcgatc ctgaaagcca
      181 tgttctgata gggcggatgt ccgccaggat gacgggcctg cgccgggatc ccgcagtgat
      241 acctgcgaat gtcacggtga gcctgccgaa ccgtttcgcc ggtccggtcc gggatcatcg
      301 tggcctaccc cgccacctcc gccgtcttct ccagcagctt tccgccttcg cagcgcacca
      361 cgcgcgcgtt caacttcttc atgtgcgtat agtcgtgcgt ggccatgatc acactgctgc
      421 cgaactggct gatggtgtac agcaggtcca ggatctctcc cgtggtttcg ggatccaggt
      481 tgcccgtggg ctcgtcggcc aggatcagtt ccggacggtt caccagtgct cgggcgatgc
      541 tcacgcgctg ttgttccccg ccgctcagct cgtgcggcat cttgtagccc ttgttgccga
      601 ggcccacctg ctccagcacc tccttcacgc gggcatcgat ctccttgcgg tccttccaac
      661 ccgtgctttc catcacgaag acgaggttcg cgttggcgtt gcggtcgctc aacaactgga
      721 aatcctggaa gacgatgccc agcttgcggc gcaggtaggg cacttccctg cgtttgagct
      781 tgcgcaggtc gtagccgcac acatgccctt cgccctgcgt cagcggcaga tcgccgtaca
      841 acgtgcgcat caagctgctc ttgccgctgc cggttcgacc gatgaggtag atgaactcgc
      901 ccttgcgcac actgaacgac acatcatcca ggatcaggtt ctcgcgttgg aagatgcgag
      961 ccgcgttcaa ttcgatgatc gggccgtcgg tggtcatgat cgcgtgatgc ggcggtattg
     1021 gaccggcgaa aatagcggcg gctcagcggc gcatacggcc aacg
//
LOCUS       DAOQOC010001268         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_142051_length_1061_cov_2.768390, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001268 DAOQOC010000000
VERSION     DAOQOC010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142051_length_1061_cov_2.768390"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PL106_16080"
     CDS             complement(<1..662)
                     /locus_tag="PL106_16080"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNA34649.1"
                     /translation="MKFSHLHVHTQFSLLDGAAAIPALFKKAVADGQPAMAITDHGNM
                     FGAFKFVAEAGNHKASAGSAGHDGLAVKPIVGCEFYVVEDRHRKQFTREEKDKRFHQL
                     MLAKNAQGYKNLSKLCSLGYIDGFYSKYPRIDKELILQYHEGLIATTCCLGASVPQAI
                     MRAGVNGDLSKAETEFKWWLDLFGEDYYIELQRHGLPEQDQVNTVLVDWARKYNVPII
                     AS"
BASE COUNT          198 a          297 c          340 g          226 t
ORIGIN      
        1 ttgctggcga tgatgggcac gttgtacttg cgcgcccagt ccaccagcac ggtgttcacc
       61 tggtcctgct cgggcagtcc gtggcgttgc agttcgatgt agtagtcctc gccgaagaga
      121 tcgagccacc atttgaactc ggtctcggcc ttggacagat caccgttcac gcctgcgcgc
      181 atgatcgcct gcggtacgct ggcacccagg cagcacgtgg tggcgatgag tccctcgtgg
      241 tattgcagga tcagttcctt gtcaatacgc gggtacttgc tgtagaagcc gtcgatatag
      301 ccgagcgagc agagcttgct gaggttcttg tagccctgcg cgttcttggc cagcatcagc
      361 tggtggaagc gcttgtcctt ctcctcgcgc gtgaattgct tgcggtgcct gtcctccacc
      421 acgtagaact cgcagcccac gatgggcttc accgcaagtc catcatgtcc ggctgaacct
      481 gccgaagcct tgtggttgcc ggcctcggcc acgaatttga acgcaccgaa catgttgccg
      541 tgatcggtga tcgccatggc gggctggcca tcggcgacgg ctttcttgaa cagcgcgggg
      601 atggcggcgg cgccatcgag cagggagaac tgagtatgta cgtggagatg gctgaacttc
      661 attggaacgg tgtggatccc gccttcagcg ggacaggcag tggaggtggg agaacttcgt
      721 catcgcgatg gggaggcgaa gctaccggag gtagggggag gaggaggtca tcagttattc
      781 acatgatcca tcctgaacct ggtctgcatc cgcatcacgc tcattgatgg tatcacgatc
      841 cgtgatgcaa tccttgaccc acggccagcg ggccctgaga ggagcagaac ggtgtggttg
      901 agttgtttgg tggtatcact gtctgtgata ccatcgccgc atatggtccg ccgggcacca
      961 gcccggccca ccagcaaata cgccgggctg gtgcccggcg gaccatactt taggagcatg
     1021 gccggaaagg agaacaatag ctacggcacg ctacgcgctg g
//
LOCUS       DAOQOC010001269         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_142421_length_1060_cov_2.366169, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001269 DAOQOC010000000
VERSION     DAOQOC010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142421_length_1060_cov_2.366169"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..>1060
                     /locus_tag="PL106_16085"
     CDS             189..>1060
                     /locus_tag="PL106_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34650.1"
                     /translation="MLLFAVLGAIVLLRRDRVRAVASLVYWVVNTYIIASWWIWYYGS
                     GWGARVYIDHYPVLFVPLAHMLSYWHGTHRRMWRATTIGLAATCLFTMAQFYQYNHNL
                     LDVEGMDARKYAYAFLRFDDAHRDRLGGMYRVPPYHPDGLDTLMHEKWDAEGSWTHWD
                     ARRIRMSGAPSATHAVACDLVDAFGPSFTLEAHEFPAGRELYLAIGFERYVEHMDDTR
                     DVLCVAVTEDTAGTQSFYSSFRMEPLPPGGEHVWEHLEYRVPYPATHGTEDLKFYFWN
                     QKSNGRFRLDDLDVT"
BASE COUNT          179 a          308 c          352 g          221 t
ORIGIN      
        1 gtcggtgggc tgtgaccgcc gctgcgatcg cgttcggcgg ggtggtcctc atccagagcg
       61 cgttgtggta cgcgcaggtc gggcatttca tcgcgtacgg gtacaaaggg gagggattcc
      121 attgggatcg gccggcgctg ttccaagtgc tcattgggtt ccgccgtggg ctgttcctct
      181 ggactcccgt gctccttttc gcggtgctcg gggcgatcgt actcttacga agggatcgtg
      241 ttcgggcggt ggcctccctg gtgtactggg tggtgaacac gtacatcatc gcgagctggt
      301 ggatctggta ttacggcagc ggctggggcg cgcgcgtgta catcgaccac tatccggtgc
      361 tcttcgtacc cctcgcgcac atgctctcgt actggcatgg aacgcatcgc aggatgtggc
      421 gcgcaaccac catcggcctc gccgcgacct gcctcttcac gatggcgcaa ttctaccagt
      481 acaaccacaa cctgctcgat gtggaaggca tggacgcacg gaagtacgcg tacgcgttcc
      541 tgcgcttcga cgatgcccat cgcgaccggc tcggtggcat gtaccgtgta ccgccctacc
      601 atcccgatgg gctggataca ctgatgcacg agaagtggga tgcggaaggt tcgtggacgc
      661 attgggatgc ccggcgcatc cgcatgtcgg gcgcaccatc ggccacgcat gccgtggcat
      721 gcgatctcgt ggacgcgttc ggaccctcgt tcacgttgga agcgcacgag ttcccggccg
      781 gtcgtgaatt gtacctggcg atcggattcg aacggtacgt ggagcatatg gacgacacac
      841 gcgatgtgct gtgtgtggcg gtgaccgagg atacggccgg aacgcaatcg ttctattcat
      901 cattccggat ggagccgctc cccccgggcg gggagcacgt gtgggagcac ctggagtacc
      961 gcgtgcccta tccggcaacg catgggacgg aggacctgaa attctacttc tggaaccaga
     1021 agagcaatgg gcggttccgg cttgatgacc tggacgttac
//
LOCUS       DAOQOC010001270         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_143862_length_1055_cov_2.329000, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001270 DAOQOC010000000
VERSION     DAOQOC010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143862_length_1055_cov_2.329000"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PL106_16090"
     CDS             <1..698
                     /locus_tag="PL106_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34651.1"
                     /translation="LELLRGRKRTWLLVLPPTIALLFFLKFYVLLSLVPALGAYAWCA
                     RTGARQPLLKSGIAFTTFLVLGLNSDLFYPNFRMLEVLYVKQRDFIGMATAAHSDSFV
                     DVTPLEPNLRSFISQAPHALYMTFLSPLTAWRTGVLGTLSAVENLWLLILPLLAFRWR
                     RPQAQVDKPLLFFCLGFCILLALVIGWTTPVIGAIVRYRVPLLPFLIMACLCIADPAR
                     VPWPSWMRIPSDQ"
     gene            725..>1055
                     /locus_tag="PL106_16095"
     CDS             725..>1055
                     /locus_tag="PL106_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34652.1"
                     /translation="MERTVIRNMDEHGRMPARKSGLMRTRVHGHIAILLAFALSSCAF
                     KNKDADLVIHNARIISLDPEGHEYQAMAIKDGRIVELGPERLILNGYSAKESYDAVDV
                     AMTEQLIA"
BASE COUNT          185 a          359 c          293 g          218 t
ORIGIN      
        1 tccttgagct gcttcgtggg cggaagcgca cgtggctgct cgtgctcccg cccaccatcg
       61 cgctgctgtt cttcctgaag ttctacgtgc tgctcagcct tgtgcccgcc ctgggcgcgt
      121 atgcctggtg cgcgcggacc ggagcccgcc agcccctgtt gaagtccggc atcgcgttca
      181 ccacattcct tgttctcggt ctgaacagcg acctattcta tccgaatttc aggatgctcg
      241 aagtgttgta cgtgaagcaa cgcgacttca tcggcatggc caccgccgcg cattccgaca
      301 gcttcgtgga cgtcaccccg ttggagccga acctccggag cttcatcagc caggcaccgc
      361 acgcgctgta catgaccttc ctctcgcccc tcaccgcatg gcgcaccggg gtgctgggca
      421 cattgagcgc cgtggagaac ctgtggctcc tgatccttcc cctgctcgcc ttccgctggc
      481 gcagacctca ggcgcaggtg gacaagccgt tgctcttctt ctgcctcggc ttctgcatcc
      541 tgctggccct ggtgatcggc tggaccacac ccgtgatcgg cgcgatcgtg cggtaccgcg
      601 tccccctctt gcccttcctg atcatggcct gcctgtgcat cgctgatcct gcgcgcgtgc
      661 catggccatc ttggatgagg ataccatcgg atcagtaaca ccatcaaaaa taccaccctt
      721 gggaatggaa cgcacagtga tccggaacat ggacgaacat gggcgcatgc ccgcacggaa
      781 aagtgggctc atgcgcacac gtgtgcatgg tcacatcgcc atcctcctgg ctttcgccct
      841 ctcctcgtgc gccttcaaga acaaggatgc cgacctggtg atccacaatg cccgcatcat
      901 atcgctcgat cccgaagggc acgaatacca ggccatggcg atcaaagatg gacgcatcgt
      961 ggaactcggg cccgagcgtc tcatcctgaa cggatactcc gccaaggaga gctacgacgc
     1021 ggtcgacgtg gcgatgaccg agcagctgat cgccg
//
LOCUS       DAOQOC010001271         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_143863_length_1055_cov_2.306000, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001271 DAOQOC010000000
VERSION     DAOQOC010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143863_length_1055_cov_2.306000"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PL106_16100"
     CDS             complement(<1..361)
                     /locus_tag="PL106_16100"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNA34653.1"
                     /translation="MKRYTEATFLIEPLDPWRDLLIAELGELGYDSFEETSNGVNAYI
                     SADRFDRAALHQLDIARQPHVRISHTVHEVEPRNWNAEWERSFQPVEVGTEVRIRADF
                     HPFVPGSKHDIVITPRMA"
     gene            complement(371..>1055)
                     /gene="tpiA"
                     /locus_tag="PL106_16105"
     CDS             complement(371..>1055)
                     /gene="tpiA"
                     /locus_tag="PL106_16105"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNA34654.1"
                     /translation="KDMPAGPAVVIAPAFPFLGIAVERTRGSRIAVAAQNCHQAAQGA
                     YTGEVSVPMLKSLGVSHCIVGHSERRQYFGETDAAVGEKIGALLEHGITPIYCCGERK
                     EEREGGRHFDVVGTQLKAALGGLAPDRIGRIIVAYEPVWAIGTGLTATAQQAQDMHAH
                     IRSLLGTHGDVVAREVPILYGGSCNPKNAEELFACPDVNGGLIGGAALIAEQFLDLVR
                     IAQLVKHLN"
BASE COUNT          177 a          344 c          338 g          196 t
ORIGIN      
        1 aggccatgcg cggggtgatg acgatatcgt gcttggatcc gggtacgaat gggtggaagt
       61 cggcccggat gcgcacctcg gtgcccacct ccacgggctg gaaactccgc tcccattcgg
      121 cgttccaatt gcggggctcc acctcatgca cggtgtggct gatgcggacg tgcggttggc
      181 gggcgatatc caactggtgc agtgcggccc tgtcgaaccg gtccgcggat atgtaagcat
      241 tgacaccgtt ggaggtctcc tcaaagctgt cgtaccccag ctcccccaat tcggcgatca
      301 gcaggtcgcg ccacggatcc aacggctcga tcaggaaggt ggcctccgtg tagcgcttca
      361 tggccgagga tcagttgagg tgtttcacga gctgcgcgat gcgaacaagg tcgaggaatt
      421 gctccgcgat gagcgcggca ccgccgatga ggccgccgtt cacatcgggg caagcgaaca
      481 gttcttccgc gttcttcggg ttgcacgatc cgccatagag gatcggcact tcgcgggcca
      541 cgacatcccc gtgcgtgccc aagagcgagc ggatgtgcgc gtgcatgtcc tgcgcctgtt
      601 gtgcggtcgc ggtgaggcct gtaccgatgg cccacacggg ttcataggcc acgatgatgc
      661 gaccgatgcg atcgggagcg agcccgccga gcgctgcctt gagttgcgtg ccgaccacgt
      721 cgaaatggcg acctccttcc cgctcctcct tgcgttcgcc gcagcaatag atgggcgtga
      781 tcccgtgttc cagcagcgca ccgatcttct cgcccacggc cgcatccgtt tcaccgaaat
      841 actgccgacg ctcgctgtgg ccaacgatgc agtgcgatac gccgaggctc ttcagcatgg
      901 gcacgctcac ctcgccggta tacgccccct gcgccgcctg gtggcagttc tgggccgcca
      961 cggcgatccg cgatccacgc gtgcgctcaa ccgcgatccc gaggaatggg aatgcaggcg
     1021 cgatcaccac ggccgggccc gcaggcatgt ccttc
//
LOCUS       DAOQOC010001272         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_144057_length_1054_cov_3.194194, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001272 DAOQOC010000000
VERSION     DAOQOC010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144057_length_1054_cov_3.194194"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1054
                     /locus_tag="PL106_16110"
     CDS             <1..>1054
                     /locus_tag="PL106_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34655.1"
                     /translation="PFPVGTPVTLTLVNDSNSLCNVNLGVLENAFCPIIIDCADSVIY
                     EDSYCYQLSDFHHWLYTSTSGAPLVIQFLQGTIESASWDHLSIYDGSDNTGTLLWTNG
                     FLTTNLTDTTVVSIGDSIYMEMSSDPSISCSSGSMTEWIWNVYCLDCTPPTATFEMVP
                     DCIHNGFSVDVNVTDLGQNTTDLLIRNTFNGDTLTGVHTGSYTVGPFPLGATGNVVVQ
                     SVNTPLCKQVSPAYFLAPDSCVTLACDAVGQEYCYSNADTAWFVYASGTSDPITVTFE
                     YGQLLVNDYIQVYNGLDTSAQIVYMGNQGGQIGGLSISSSNPANALTVLVISSQAGSC
                     STGQASPALYWSVSCGL"
BASE COUNT          215 a          323 c          289 g          227 t
ORIGIN      
        1 cccttccccg ttggcacacc ggtcacgctc accttggtga acgactccaa ctccctgtgc
       61 aacgtgaacc tcggtgtcct ggagaatgca ttctgcccga tcatcatcga ttgcgcggac
      121 tccgtgatct acgaggacag ctactgctat caactcagcg atttccacca ctggctgtac
      181 accagcacga gcggcgcgcc actggtgatc cagttcctgc aaggaaccat cgagagtgcc
      241 tcgtgggatc atctgtccat ctacgatgga tcggacaaca cgggaaccct gctctggacg
      301 aacggtttct taacgaccaa cctcacggat accaccgtgg tcagcattgg cgactcgatc
      361 tacatggaaa tgagttctga cccgagcatc tcttgttcca gcggtagcat gaccgagtgg
      421 atctggaacg tgtactgcct ggactgcacc ccgcccacgg ccaccttcga aatggtgccc
      481 gattgcatcc acaatggatt ctcggttgat gtgaatgtga ccgacctcgg ccagaacacc
      541 acggatctcc tcatccgcaa taccttcaat ggcgacaccc ttacaggtgt tcataccggg
      601 tcgtacacgg ttggaccctt ccccttgggc gcaacgggga atgtggtcgt gcagagcgtg
      661 aacaccccct tgtgcaaaca ggtgagccct gcgtatttct tggcgcccga tagctgtgtc
      721 accctcgcat gcgatgccgt gggccaggag tattgctact ccaatgcgga taccgcatgg
      781 ttcgtgtacg ccagtggtac ctcggacccc atcacggtca ccttcgagta cgggcagctt
      841 ctggtcaacg actacatcca agtgtacaac gggttggata ccagtgcgca gatcgtgtac
      901 atgggcaacc agggtgggca gatcggaggt ctgtccattt cgagcagcaa tccggccaac
      961 gcccttaccg tgctggtgat cagcagccag gcgggctcct gttctaccgg gcaggcctcg
     1021 ccggcgctgt actggagcgt gagttgcgga ttgg
//
LOCUS       DAOQOC010001273         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_144140_length_1054_cov_2.406406, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001273 DAOQOC010000000
VERSION     DAOQOC010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144140_length_1054_cov_2.406406"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1054
                     /locus_tag="PL106_16115"
     CDS             <1..>1054
                     /locus_tag="PL106_16115"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNA34656.1"
                     /translation="CMIDSTELIWSVLDSGTAEVNGSLTFNADGTFTYAPDPGYAGEV
                     SFTYTVCDTVPACDTALVTITITMPTPTPENDDCADMSADTLEIDDTLIFTGDNTGAT
                     IDGDYEPGSPMEADSLPSVWHVFATEECADVIVSYCGTAPAFQNVWTLLTTGCPGGAN
                     IVPADSVNGEDCGDGNTWIAFYDLPAGTYYLPVKLDTGSASPAVGPYSIQVSASACEV
                     PPAPPVNDTCTGAVVLNVNLDCQPVFGTVQDATESLPAILCNELEGWANDDVWYSFVA
                     TNPHQTISVTGSEEFDPVVELFEGTCDNMVSLACSDTSVLGGTEAIELFDMVEGTNYL
                     VRLYDYGEGYPLDPTFS"
BASE COUNT          213 a          323 c          318 g          200 t
ORIGIN      
        1 tgcatgatcg acagcaccga gctgatctgg tccgtattgg acagcggcac agccgaagtg
       61 aatggctcgc tcacgttcaa tgcggacggc acgttcactt acgcaccgga ccccggatat
      121 gcgggtgaag tttcgttcac ctataccgtg tgcgacaccg tgccggcttg cgacacggcc
      181 ctggtgacca tcacgatcac gatgccgacc ccgaccccgg agaacgacga ctgtgccgac
      241 atgtctgccg acaccttgga gatcgacgat acgctcatct tcacgggcga taacacggga
      301 gccacgatcg acggtgatta cgaacccggc tcgccgatgg aagcggacag cctgcccagc
      361 gtatggcatg tgttcgccac ggaggagtgt gccgatgtga tcgtatccta ctgcggaacc
      421 gcgccggcct tccagaacgt gtggactttg ctgaccacgg gttgcccagg cggtgcgaac
      481 atcgtaccgg ctgattcggt gaacggcgag gattgtggtg atggcaatac gtggatcgcg
      541 ttctacgacc tgcccgccgg cacctattac ctgcccgtga aactggacac cggttcggcc
      601 agccccgcag tgggcccata cagtatacag gtgagcgctt cggcctgcga ggtaccgccc
      661 gcgccgcccg tgaacgacac gtgtaccggt gccgtggtat tgaacgtgaa cctggactgc
      721 caaccggtgt tcggcaccgt tcaggatgcc acagagtcgc tgcccgccat cctctgcaac
      781 gagttagagg gctgggccaa cgacgatgta tggtacagct tcgtggccac caatccgcac
      841 cagaccatca gtgtgaccgg aagtgaggaa ttcgacccgg ttgtggaact cttcgagggc
      901 acgtgcgaca acatggtgag cctcgcctgc tcggatacct cggttctggg aggcaccgaa
      961 gcgatcgaac tgttcgacat ggtggaaggc accaactacc tcgtccgttt gtacgattac
     1021 ggcgagggtt atccgctcga ccccaccttc agca
//
LOCUS       DAOQOC010001274         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_144288_length_1053_cov_4.900802, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001274 DAOQOC010000000
VERSION     DAOQOC010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144288_length_1053_cov_4.900802"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="rpsL"
                     /locus_tag="PL106_16120"
     CDS             <1..362
                     /gene="rpsL"
                     /locus_tag="PL106_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNA34657.1"
                     /translation="KGRTAPTYKSKSVALTECPQRRGVCTKVYTTTPKKPNSALRKVA
                     KVRLTNKYEVIAYIGGEGHNLQEHSIVLVRGGRVKDLPGVKYHIVRGVLDTAGVEGRG
                     QRRSKYGTKKKKAAAKK"
     gene            411..875
                     /gene="rpsG"
                     /locus_tag="PL106_16125"
     CDS             411..875
                     /gene="rpsG"
                     /locus_tag="PL106_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNA34658.1"
                     /translation="MRKKAVKHTTLPDPRFGDTQVTKFVNNLMYDGKKNKSFDIFYDA
                     IDIVSEKTGEEGLEIFRKAITNVTPQVEVRSRRVGGANFQIPQPVREGRKLSLAMKWL
                     IGYSRGRNERSMAQKLANEIMAAAKEEGAAFKKKEEVHKMAEANKAFSHFRF"
     gene            900..>1053
                     /locus_tag="PL106_16130"
     CDS             900..>1053
                     /locus_tag="PL106_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNA34659.1"
                     /translation="MARDLKYTRNIGIMAHIDAGKTTTSERILYYTGLTHKIGEVHDG
                     AATMDWM"
BASE COUNT          270 a          320 c          321 g          142 t
ORIGIN      
        1 gcaagggccg taccgccccg acctacaaga gcaagtcggt ggccctcacc gagtgcccgc
       61 aacgtcgcgg tgtgtgtacc aaggtgtaca ccaccacgcc gaagaagccg aactcggccc
      121 tccgcaaagt ggccaaggtg cgcctcacca acaagtacga ggtgatcgcc tacatcggtg
      181 gcgaggggca caacctgcag gagcacagca tcgtgctggt gcgcggtggc agggtgaagg
      241 acctccccgg ggtgaagtat catatcgtgc gcggagtgct ggataccgcg ggtgttgaag
      301 gtcgtggcca gcgtcgcagc aagtacggca ccaagaagaa gaaggccgcc gccaagaagt
      361 aaggcgaagg tcatccaacg atagtccgac agcaagacaa cgaacgagca atgcgcaaga
      421 aggcagtcaa gcacaccacc cttcccgacc cacggttcgg ggacacgcag gtgaccaagt
      481 tcgtgaacaa cctgatgtac gacggcaaga agaacaagtc gttcgacatc ttctacgacg
      541 ccatcgatat cgtgagcgag aagacgggcg aagagggcct ggagatcttc cgtaaggcga
      601 tcacgaacgt tactccgcaa gtggaggtgc gcagccgccg cgtgggcggg gccaacttcc
      661 agatcccgca gcccgtgcgc gagggtcgca agctgagcct ggccatgaaa tggctgatcg
      721 gctacagccg cggccgcaac gagcgcagca tggcccagaa gctcgccaac gagatcatgg
      781 ccgccgccaa ggaagagggc gccgcattca agaagaaaga agaagtccac aaaatggccg
      841 aagcgaacaa ggccttcagc cacttccgct tctaacctca gccaacgaga gcacgccaca
      901 tggcccgcga cctcaaatac acgcgcaaca tcggcatcat ggcgcacatc gatgccggca
      961 agaccaccac gtccgagcgc atcctgtact acaccggctt gacccacaag atcggtgagg
     1021 tgcatgatgg tgccgctacc atggactgga tgg
//
LOCUS       DAOQOC010001275         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_144813_length_1052_cov_1.464393, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001275 DAOQOC010000000
VERSION     DAOQOC010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144813_length_1052_cov_1.464393"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PL106_16135"
     CDS             complement(<1..900)
                     /locus_tag="PL106_16135"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNA34660.1"
                     /translation="MKVIDHLRQAAAASKPRTLLSFEILPPLKGKDIRSIYAGIDPLL
                     EFKPSFINVTYHREEVVYKERGHGLLQKIRIRKRPGTVGICATIKAKYNIDTVPHLIC
                     GGFSKEDTEDALIELNFLGIDNVLALRGDAIKNEPHFIPERDGHAHAEDLILQISALN
                     QGKYLYEEEQEAAATDLCIGAACYPEKHAEALNLTTDIAYLKRKVDAGADYLVTQMFF
                     DNRKYFRFVELCREAGIQVPIIPGLKPITNLKHIAFIPKTFAIDLPEDMARELVKCRT
                     DAEVKEVGVAWSIAQARELMQAGA"
BASE COUNT          161 a          307 c          362 g          222 t
ORIGIN      
        1 ggcgccggcc tgcatgagct cgcgggcctg tgcgatgctc caggccacac cgacctcctt
       61 cacctccgca tccgtgcggc acttcaccag ttcgcgcgcc atgtcctcgg gcagatcgat
      121 ggcgaaggtc ttcgggatga aggcgatgtg cttgaggttc gtgatcggtt tcagtccggg
      181 gatgatgggg acctggatcc cggcctccct gcacagctcc acgaaccgga agtacttccg
      241 gttgtcgaag aacatctggg tcacgaggta gtccgcaccg gcgtccacct tgcgctttag
      301 gtacgcgata tcggtggtga ggttcagtgc ctccgcgtgc ttctcggggt agcatgccgc
      361 gccgatgcag aggtcggtgg ccgcggcctc ctgctcctcc tcgtacaggt acttgccctg
      421 gttcagtgcg gagatctgca ggatcaggtc ctcggcatgg gcgtggccat cgcgctccgg
      481 gatgaagtgc ggttcgttct tgatggcatc tccgcgcagg gcgagcacgt tgtcgatccc
      541 caggaagttc agctcgatca acgcgtcctc ggtgtcctcc ttgctgaagc cgccgcagat
      601 caggtgcggc accgtgtcga tgttgtactt ggccttgatg gtggcgcaga tgcccacggt
      661 gccgggccgc ttccgtatgc ggatcttctg cagcaggcca tgaccgcgct ccttgtacac
      721 cacttcctcg cggtgatagg tcacgttgat gaagctgggc ttgaactcca gcaacgggtc
      781 gatgccggcg tagatggagc ggatgtcctt gcctttgagc ggcggcagga tctcgaagga
      841 caacagggtt cgcggcttgc ttgcggcggc ggcctggcgg agatggtcga tgaccttcat
      901 cgggtgggat cggagggcgg cgaagatacc ggggtgcgta ttgaagcacg aacgccccgg
      961 ttgccggggc gttcgcggaa gagggagggg atgctcagcg cagcagcgtc acgtggccgc
     1021 tgtagtcctt gccgttgggc agtttcagca cg
//
LOCUS       DAOQOC010001276         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_144901_length_1051_cov_3.385542, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001276 DAOQOC010000000
VERSION     DAOQOC010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144901_length_1051_cov_3.385542"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1051)
                     /locus_tag="PL106_16140"
     CDS             complement(<1..>1051)
                     /locus_tag="PL106_16140"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNA34661.1"
                     /translation="AKLAAYEYENYNKVEFDLNNITEEFKKKKLFKDLEFIFEYVDTT
                     TRKPSLPIFMTETVSDVYYRQEPRTRREIIKGNRVSGVENESITQFMGDMYQNVNIYE
                     NYLELFGKNFTSPIADGGKAHYDYLLVDSNWVGRNWCYLIRFKPKHRQQLCFTGEMWI
                     NDTSFAVRRIEGGIEPGTNVNFVQELRVRQEYEEVAREVWMLTRDELLVDLNPVRDTG
                     EVSKRPLQGFYGRRTATYRDFVINKPRDDAFYRGMSEVVVDTDPLSLGADWWDTHRHE
                     QLSKRENDIYHMVDTMRTVPRFKTYLDIITTVVSGYYVTGPVEIGPYFNIFSFNPVEG
                     PRFRLGARTSNAFSRR"
BASE COUNT          181 a          335 c          293 g          242 t
ORIGIN      
        1 cgcctgctga aggcgttgct ggtgcgcgca cccagccgga agcgcgggcc ttccaccggg
       61 ttgaaactga agatgttgaa gtacgggccg atctccaccg gccccgtgac gtagtagccc
      121 gagaccaccg ttgtgatgat gtccagatac gtcttgaagc gcggcaccgt cctcatcgtg
      181 tccaccatgt ggtagatgtc gttctcgcgc ttgctcaact gctcgtgccg gtgcgtgtcc
      241 caccagtcgg cacccaggct caacgggtcc gtatcaacga ccacttcgct catacccctg
      301 taaaaggcgt catcacgcgg cttgttgatc acgaagtccc ggtatgttgc ggtacgacgg
      361 ccgtaaaagc cctggagggg ccgcttgctc acctctcccg tgtcccgtac ggggttcagg
      421 tccaccagca gctcgtcgcg cgtgagcatc cacacctcac gggccacctc ctcgtactcc
      481 tgccgcaccc gcaactcctg aacgaagttg acgttggtgc ccggctcgat gccgccctcg
      541 atgcgccgca ccgcgaaact ggtatcgttg atccacatct cgccggtgaa gcacaactgc
      601 tgccggtgtt tcggcttgaa tcggatgagg tagcaccagt tccggcccac ccaattgctg
      661 tccaccagca gataatcgta gtgcgccttg ccgccgtcgg cgatcgggct tgtgaaattc
      721 ttgccgaaca gctccagata gttctcgtag atgttcacgt tctggtacat gtcgcccatg
      781 aactgggtga tgctctcgtt ctccaccccg ctcacacgat tgcccttgat gatctcgcgc
      841 ctggtgcggg gctcctgccg gtagtacacg tcgctcaccg tctcggtcat gaagatgggc
      901 agcgagggct tgcgcgtggt ggtgtccacg tactcgaaga tgaattccag gtccttgaag
      961 agcttcttct tcttgaattc ctcggtgatg ttgttcaggt cgaactccac cttgttgtag
     1021 ttctcgtact cgtacgcggc cagcttggca c
//
LOCUS       DAOQOC010001277         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_145828_length_1048_cov_2.397784, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001277 DAOQOC010000000
VERSION     DAOQOC010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145828_length_1048_cov_2.397784"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PL106_16145"
     CDS             complement(<1..194)
                     /locus_tag="PL106_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34662.1"
                     /translation="MRGRNETLFLKAQLGYTKEFGIRYRFPYISRRQRWGLAFGGSRT
                     HQDEITAGTRGNKREFITLHG"
     assembly_gap    221..242
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<243..648)
                     /locus_tag="PL106_16150"
     CDS             complement(<243..648)
                     /locus_tag="PL106_16150"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNA34663.1"
                     /translation="MRFFRILVLLAAIGAAHVAKPQPDPVWNGPYTIHGVVIEGTRAT
                     KDGVILRELTLAEGDTVASAEQLNAMIERSRQNVFNLGLFHSVEIMPLYLSSTDVFLT
                     ITVVERWAWRPSPIFRYSDPNFNTWWLTRELRR"
     gene            complement(705..>1048)
                     /locus_tag="PL106_16155"
     CDS             complement(705..>1048)
                     /locus_tag="PL106_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34664.1"
                     /translation="VNEHTALLRLSEVTNVREPGSVLVLEVNAIDYSLQQIARIVEGN
                     DAKVLSVYCHTLPDSRRMEVVLKINREDISGVLQTFERYEYEVKTTYQGSRFHEDLRG
                     RYDELMRLIDL"
BASE COUNT          181 a          332 c          314 g          199 t
ORIGIN      
        1 ccatgcaggg tgatgaactc ccgcttgttg cccctggtgc ccgccgtgat ctcgtcctga
       61 tgcgtgcgcg aacccccgaa ggcgaggccc cagcgctgcc gacgactgat gtacgggaac
      121 cggtagcgta tgccgaactc cttggtgtaa cccagctgcg ccttcaggaa gagcgtctcg
      181 ttgcggccgc gcatgttgag ccggctcagg tacaggccgt nnnnnnnnnn nnnnnnnnnn
      241 nncccgccgc agttcgcgcg tgagccacca ggtgttgaag ttggggtcgc tgtaccggaa
      301 gatcggcgag ggccgccagg cccagcgttc caccacggtg atcgtgagga aaacatcggt
      361 ggacgagagg tacagcggca tgatctccac gctgtggaac agccccaggt tgaacacgtt
      421 ctgccggctg cgctcgatca tggcgttcag ctgttcggca ctggccaccg tatcgccctc
      481 ggccagggtg agttcgcgca ggatcacccc gtccttggtg gcgcgcgtcc cttcgatcac
      541 cactccatga atggtgtacg gtccgttcca cacgggatcc ggttggggtt tcgcaacgtg
      601 cgccgcgccg atcgcagcaa ggaggaccag gatccggaaa aaacgcatgc ccaaggttcc
      661 gtgttcacgt gcgtgcgccc agcggcgaga acgtggcccg gggatcacag atcgatcagg
      721 cgcatcagtt catcatagcg cccccgcagg tcctcatgga agcgtgaacc ctgataggtg
      781 gtcttcacct cgtactcgta ccgctcgaag gtctgcagca caccgctgat gtcctcgcga
      841 ttgatcttca ggaccacttc catccgccta ctgtccggca gggtgtggca atacacactg
      901 agcaccttcg cgtcgttacc ttccacgatc ctggcgatct gctggagact gtagtcgatg
      961 gcgttcacct ccagcaccag cacgctgccg ggttcgcgca cgttggtcac ctcgctcagg
     1021 cgcaacaagg ccgtgtgctc gttcaccg
//
LOCUS       DAOQOC010001278         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_146016_length_1047_cov_4.017137, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001278 DAOQOC010000000
VERSION     DAOQOC010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146016_length_1047_cov_4.017137"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1047
                     /locus_tag="PL106_16160"
     CDS             <1..>1047
                     /locus_tag="PL106_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34665.1"
                     /translation="EILAHLAEDQVSLGDTIGALRSFDRSRALWPRTDSLNQALLGSQ
                     YTQLLLRLGREREAVAEFERMRNICAARAIPLASHHGVCELDYCSFLLNSANGDQRMA
                     DSSLHRALAEAARLNELPLVLKYRKALMHRMLAQGDVRGAAEQSAAYVRLNDSLQAVA
                     NADAVAAYEGQVQERESALEIQRQQARVKQQRMVLLGSTIVLLLLAALAWSVYKGKKR
                     SDELLLNILPAEVAKELKATGASAARHIEHATILFTDFKGFTEASELLTPQELVEELD
                     TCFKAFDAIITTRGIEKIKTIGDAYMAAGGLPRKVASGVGEVVQAALDMQDFMLKRKA
                     QRTALGRPAFDMRVG"
BASE COUNT          194 a          332 c          352 g          169 t
ORIGIN      
        1 gagatcctcg cgcacctggc cgaggatcag gtcagcttgg gcgatacgat cggtgccctc
       61 cgctccttcg atcggtcgcg cgcgctgtgg ccccgtaccg acagcctcaa ccaggcgctc
      121 ctgggttcgc agtacacaca actgctcctt cgcctggggc gtgagcggga ggcggtggcc
      181 gagttcgagc ggatgcggaa catctgtgcc gcgcgagcga tcccactggc gtcccatcat
      241 ggtgtgtgcg aactggacta ttgtagcttc ctgctgaaca gcgccaacgg tgaccagcgc
      301 atggccgata gcagtctgca ccgcgctctg gcggaagccg cacggctgaa cgagctgccc
      361 ctggtgctga agtaccggaa agcgctgatg caccgcatgc tcgcccaggg cgatgtccgt
      421 ggcgcggccg aacagtccgc cgcctacgtg cgcctgaatg actccctgca ggcggtagcg
      481 aacgccgatg cggtggccgc ctacgaaggg caggtgcagg aacgggagag cgcgctggag
      541 atccaacgcc aacaggctcg agtgaagcaa caacgcatgg tgttgctcgg cagcaccatc
      601 gtgctgctgc tccttgcggc gctcgcctgg tccgtttaca agggcaagaa gcgctcggat
      661 gaattgctgc tcaacatcct tccggcggag gtcgcgaagg aactgaaggc taccggggcc
      721 agcgccgcgc gccacatcga acacgccacc atcctcttca ccgatttcaa gggcttcacc
      781 gaggcgagcg agctgcttac gccgcaggag ctggtggaag agctggacac ctgcttcaag
      841 gccttcgatg ccatcatcac cacgcgcggc atcgagaaga tcaagaccat cggtgatgcg
      901 tacatggcgg ccggtggcct gccccgcaaa gtggccagcg gcgtggggga ggtggtgcag
      961 gccgcgctcg acatgcagga cttcatgctg aagcggaagg cccaacgcac cgcgctgggg
     1021 aggcccgcgt tcgacatgcg ggtgggc
//
LOCUS       DAOQOC010001279         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_146934_length_1044_cov_3.934277, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001279 DAOQOC010000000
VERSION     DAOQOC010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146934_length_1044_cov_3.934277"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PL106_16165"
     CDS             <1..834
                     /locus_tag="PL106_16165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34666.1"
                     /translation="NIIRVHPDGALDTTFQTNASWFYPTFYHPLVDGKVICTGDVVFS
                     GSDDTLHVFRLLPDGSLDPTFNNHLAMWRTTDMDGIAGVSSITPLGSDRYIVTGGFDR
                     VEGQFRGGIAVIDTAGYLLDEPLGEVGCGTFWNGFYNIGLIVGIVSDPQSGYYIFGSY
                     HGYNDGTTNDVYQRMVSRLYGLDVGVEEEAVQAGASLSLAPNPATAWCTVEYDLRTAP
                     EGASVELLDITGRSLWRAALNNAKGQTVLDTRNLASGNYMVRLLNGGAILGVRKLNVM
                     N"
     gene            838..>1044
                     /locus_tag="PL106_16170"
     CDS             838..>1044
                     /locus_tag="PL106_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34667.1"
                     /translation="MIRSRGLLMAATVGVAAIAGAQAPFDLDPSFETTINHGGVSSVA
                     VLDDGDVLVSGDIRYGTLSEWLLVR"
BASE COUNT          202 a          268 c          338 g          236 t
ORIGIN      
        1 aacatcatcc gggtccatcc ggacggtgct ttggatacga cctttcagac caacgcgagc
       61 tggttctatc cgacgttcta ccaccccctc gtggatggga aagtgatctg taccggcgat
      121 gtggtattca gtgggagcga tgacacgctc catgtgttcc gcttgttgcc ggatggctca
      181 ttggatccaa cgttcaacaa ccatctggcc atgtggcgca ccaccgatat ggatggtatc
      241 gcaggggtca gttcgataac acccctgggg tcggaccgct acatcgttac cggcggtttc
      301 gatcgggtgg aggggcagtt ccggggtggg atagctgtga tcgacacagc gggatatttg
      361 ttggatgaac cattggggga agtaggttgc ggcacgttct ggaatggttt ctacaacatc
      421 ggtttgatcg tcggcatcgt atcggacccg caaagtggct actacatttt cggcagctac
      481 cacggttaca acgacggcac caccaacgat gtgtaccagc gcatggtgag ccggttgtac
      541 gggctggatg tgggggtgga ggaggaggct gtgcaggcgg gcgcctccct gtccctggcc
      601 cccaacccgg ccacggcgtg gtgtacggtg gagtatgatc tgcgaaccgc tccagaaggt
      661 gcttcggttg aactgctcga tatcacaggg cgttcgctct ggcgtgccgc attgaacaac
      721 gccaagggac aaacggtgtt ggacacccgc aacctggctt cgggcaatta catggtgcgc
      781 ctgctgaacg gaggggcgat cctgggtgtt cggaaactga atgtgatgaa ctgagccatg
      841 atacgctcac gcggattact gatggcggcc accgtggggg tggccgccat cgcgggcgcg
      901 caagcaccgt tcgatctgga tccgtcgttt gaaacgacca tcaaccacgg tggggttagt
      961 tcggtcgccg ttcttgatga tggtgatgtc ctggtgtcgg gagatatccg gtacggtaca
     1021 ctcagtgaat ggctacttgt tcgt
//
LOCUS       DAOQOC010001280         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_147073_length_1044_cov_2.183013, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001280 DAOQOC010000000
VERSION     DAOQOC010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147073_length_1044_cov_2.183013"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PL106_16175"
     CDS             <1..412
                     /locus_tag="PL106_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNA34668.1"
                     /translation="PGDASRRPGLNMVLWAMQGLLFLAVGALGLMSLTMPIPKLADLL
                     TWPGAVPELLTRGIGAVEVAGAMGVFLPALVRMQPWITSYAAMGLTTVMICAVGYHMM
                     LFQGKAMLPSIILGVIAGYVAWGRETVLPIKGRG"
     gene            complement(585..>1044)
                     /locus_tag="PL106_16180"
     CDS             complement(585..>1044)
                     /locus_tag="PL106_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNA34669.1"
                     /translation="IAKVWPLLSNDKPQSADIRKFETLGTRSFPAEMATGYSLDLHNL
                     IGPARKQQRLHFLKSYWMDRVMDVPGIFFRTLNDPRYSCAIGTFGMEGKKATAIAEAL
                     HTKWKIHTVAIERAGIPGVEAPYNHVRVTPNVYTTTQELDRLVEAIRTMA"
BASE COUNT          158 a          304 c          357 g          225 t
ORIGIN      
        1 gccaggcgac gcctctcgcc gccccggtct gaacatggtg ctctgggcca tgcagggctt
       61 gctgttcctt gcggtcggcg cgttgggcct tatgagcctc acgatgccaa tacccaagtt
      121 ggcggattta ttgacatggc ccggggcggt ccccgagttg ctcacccggg gcatcggtgc
      181 cgtagaagtg gcgggggcca tgggcgtttt cctgcccgcg ctcgtgcgca tgcagccctg
      241 gatcaccagc tacgccgcca tgggattgac cacggtgatg atctgcgcgg tgggctacca
      301 catgatgctc ttccaaggca aggcgatgtt gccgagcatc atcctgggcg tcattgcggg
      361 ctatgtggcg tggggccgcg aaacggtgct gccgatcaag gggcgcggtt gatcgtgtgg
      421 tccggtggcc taggatccgc atcacccaaa gcgggtgcgg gtggcggtgg cgcgccccga
      481 tccaggtggg tcggacctcc ggaatgatcc gatcaaagaa tgcttcatcg cgttcggcgg
      541 aacggttcgg cggtccaccg ccagatgacg ctgaaccgtg gtgatcaggc catggtcctg
      601 atcgcctcta ccagccggtc cagttcctgg gtggtggtgt acacgttggg cgtaacgcgc
      661 acgtggttgt acggggcctc tacgcccgga atacccgcgc gttcgatggc cacggtgtgg
      721 attttccact tggtatggag tgcttcagcg atggcggtcg ccttttttcc ctccatcccg
      781 aaggtgccga tggcgcagct gtaccgcggg tcgttcagcg ttcggaagaa gatgccgggc
      841 acatccatca cgcgatccat ccaatagctc ttcaggaagt gcaggcgttg ctgcttgcgc
      901 gctggtccaa tgaggttgtg caggtcgagg gagtaaccgg tggccatctc ggccgggaag
      961 ctgcgtgtgc cgagcgtttc gaacttgcgt atgtcggcgc tttgcggctt gtcgttgctc
     1021 agcagcggcc acaccttggc gatc
//
LOCUS       DAOQOC010001281         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_147246_length_1043_cov_3.156883, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001281 DAOQOC010000000
VERSION     DAOQOC010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147246_length_1043_cov_3.156883"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1043
                     /locus_tag="PL106_16185"
     CDS             <1..>1043
                     /locus_tag="PL106_16185"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNA34670.1"
                     /translation="AQAAFVTFEGFTVGMGTTTQDDLSTGWVQGTSTVYGPTDFDNST
                     TGWNTHVFDTPWYWDGQSDLVVQTCFSNNDNAQNAFHRQSETSYTSTLVRSSNNPNVC
                     FSQQFTPYTQRPNMRFTWAAVAPEAHFTQSTTATCGGTVTFLDASTGAPTGWQWDFGD
                     GTTDSVPDPVHQYMSDGTFVPVLIVTNNAGSDTLEGAPITVNASGPRPVDACIPATEG
                     SLPGIGIVAVTLNGSTYSSGDAQSEGWVDRSCILTTVATGAVLNMTVGTGNVAQHNIR
                     GWIDWNGDGEFTGNELVLSADNQTSATGSVTVPVTAPIGTPLRMRFAADYSFSPYPQP
                     CGPLQYGQAEDHG"
BASE COUNT          214 a          331 c          328 g          170 t
ORIGIN      
        1 ggcccaagcg gccttcgtca cctttgaagg attcaccgtg ggcatgggca ccaccaccca
       61 ggacgacctg tccacgggct gggtccaagg caccagtacc gtgtacggtc caacggattt
      121 cgacaattcg accaccggtt ggaacacgca cgtctttgac acgccctggt attgggacgg
      181 ccagtcggat ctggtggtgc aaacgtgctt cagcaacaac gacaacgcgc agaacgcctt
      241 ccaccggcag tcggagacat cgtacacgag cacactggtg cgcagctcga acaaccccaa
      301 cgtctgtttc tcccagcagt tcaccccgta cacacagcgg ccgaacatgc gattcacgtg
      361 ggcggcagtg gcgcccgagg cgcatttcac ccagagcacc accgcgacct gcggcggtac
      421 ggtgactttc ctggacgcat ccactggcgc ccccacggga tggcagtggg acttcggcga
      481 cggcaccacg gattcggtac ccgaccccgt gcatcaatac atgagcgatg ggacattcgt
      541 gccggtgctg atcgttacca ataatgctgg atcggacacc cttgaagggg ccccgatcac
      601 cgtgaacgcg agcggaccgc gacccgtgga cgcctgcatc cccgccacgg agggatcgct
      661 gccgggcatc ggtatcgtgg ccgtgacgtt gaacggctcc acgtacagca gcggcgatgc
      721 gcagagtgaa ggctgggtgg accgcagttg catcctgacc accgtggcca cgggagcggt
      781 gctcaacatg acggtgggaa cgggcaacgt ggcacagcac aacatccggg gctggatcga
      841 ttggaacgga gacggggagt ttaccgggaa tgaactggtg ttgagtgccg acaaccagac
      901 ctcagccacg ggcagcgtaa cggttccggt gaccgcaccg atcggtacgc ccctccgtat
      961 gcgcttcgcg gcggattaca gcttcagccc gtatccgcag ccctgcgggc cgttgcagta
     1021 cgggcaggcg gaggatcatg gat
//
LOCUS       DAOQOC010001282         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_147852_length_1041_cov_3.484787, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001282 DAOQOC010000000
VERSION     DAOQOC010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147852_length_1041_cov_3.484787"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PL106_16190"
     CDS             <1..611
                     /locus_tag="PL106_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNA34671.1"
                     /translation="WGAGKRVLTGESLFMTTYTNTAHERRTVWFAGAFPGKIIPLDLR
                     DYGQRLICQKDSFLCAAKGVNIGIAFQRKLGTGLFGGEGFIMQKLEGDGQVYVMAGGT
                     TVQKELRAGETLRVDTGCLVALTSNVDYDIQFVGGIKNTLFGGEGLFFATLRGPGHVW
                     IQSLPFSRLADNIISASPKAGGSRKGEGSILGGLGGLLDGDN"
     gene            complement(722..>1041)
                     /locus_tag="PL106_16195"
     CDS             complement(722..>1041)
                     /locus_tag="PL106_16195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNA34672.1"
                     /translation="NQLKRYHDSEYKRPGTVGFPSVVQAPGFGAWPNPAASVLHLGLP
                     NGIVSPDIHICDATGRTVHVSGPQAARTGTIDVSSLANGLYTVSLLDGSRILGATRVV
                     VQR"
BASE COUNT          200 a          295 c          338 g          208 t
ORIGIN      
        1 tgtggggtgc gggcaagcgc gtgctcaccg gggagagcct tttcatgacg acgtatacga
       61 acacggcgca cgagcgccgt acggtctggt tcgctggggc cttcccgggg aagatcatcc
      121 cgctggacct gcgcgactat ggccaacggc tcatctgcca gaaggattcc ttcctctgcg
      181 cggccaaagg ggtgaacatc ggcatcgcgt tccagcgcaa gctcggaaca gggctgttcg
      241 gtggtgaggg cttcatcatg cagaagctcg aaggtgatgg acaggtgtac gtaatggccg
      301 ggggcaccac ggtgcagaag gagttgcgcg ccggagagac ccttcgggtc gacacgggat
      361 gcctggtggc cctgacaagc aacgtcgatt acgacatcca gttcgtaggt ggtatcaaga
      421 acaccttgtt cggaggcgag ggactcttct tcgccacgct gcgcggtccg ggacatgtct
      481 ggatccagtc cttgcccttc agccgcttgg ccgacaacat catcagcgct tcgccgaagg
      541 ccggtggcag ccgcaagggt gaaggctcga tcctgggtgg cttgggtggc ctgctggacg
      601 gagataattg agaagctaca agacccaagc ttcaatgctc aagcttcaat gctcaagctt
      661 caaattgcca accaagtgtt ttgcgggtct tgaagcttga gccttgtgtc ttgagccttg
      721 atcagcgttg taccaccacc cgcgtagcgc ccaggatccg gctgccatcc agcaggctca
      781 cggtgtagag cccattggcc aggctgctca cgtcaattgt tccggtgcgc gcggcctgcg
      841 gacccgagac atgaaccgtg cggcccgtgg catcgcagat gtgaatgtcc ggtgaaacaa
      901 taccgtttgg aaggccgagg tgcagcaccg atgccgccgg gttgggccat gcgccgaagc
      961 cgggcgcttg cacaacggaa gggaacccca ccgtgccggg tcgcttgtat tcgctgtcgt
     1021 gataacgttt cagttggttg c
//
LOCUS       DAOQOC010001283         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_148428_length_1039_cov_4.497967, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001283 DAOQOC010000000
VERSION     DAOQOC010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148428_length_1039_cov_4.497967"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PL106_16200"
     CDS             complement(<1..586)
                     /locus_tag="PL106_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNA34673.1"
                     /translation="MLTDAELYDRLATFVSDNKRALFDRIAPERTRHVTVVLEDIYQP
                     HNASAVVRTCDLLGVQDIHIIENRNKYVMNPDVTLGSSKWTDMHRYRGDGDNSLKCAE
                     HLQQAGYLIVATSPRPGSFTPHDIPLDKPLAFCFGTELTGLGPALMDRADLHMRIPMY
                     GFTESYNISVSAAITLFTVMERLRAGGDHWRLPAD"
     gene            complement(712..984)
                     /locus_tag="PL106_16205"
     CDS             complement(712..984)
                     /locus_tag="PL106_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNA34674.1"
                     /translation="MNKAELIESIATEAKISKADAKRALDAFVNNTTKALKKGDRVAL
                     VGFGTFSITKRAARKGRNPQTGKEIKIAAKKVVKFKAGADLSNKVK"
BASE COUNT          165 a          271 c          352 g          251 t
ORIGIN      
        1 catccgcggg gagccgccaa tgatccccac cggcgcgcag tcgttccatc accgtgaaca
       61 gggtgatggc ggcactgacg ctgatgttgt aactctcggt gaagccgtac atggggatcc
      121 gcatgtgcag gtcggcacgg tccatcaacg cggggccgag accggtcagt tcggtgccga
      181 agcagaaggc caggggcttg tccagcggga tgtcgtgcgg tgtgaagctg cccggacgcg
      241 ggctggtggc cacgataagg tacccggctt gctggaggtg ttccgcgcat ttgagtgaat
      301 tgtcaccatc cccacgatag cggtgcatgt cggtccactt cgaggatccg agcgtcacgt
      361 cgggattcat cacgtatttg ttccggttct cgatgatgtg gatgtcctgg accccgagca
      421 gatcgcaggt gcgtaccacc gcgctcgcgt tgtgcggttg gtagatgtcc tccagtacca
      481 cggtcacatg gcgcgtgcgt tccggagcga tacggtcgaa gagcgcacgt ttgttgtcgg
      541 atacgaacgt ggcgaggcgg tcgtagagtt cggcgtcggt gagcatgtgt tgttctcctg
      601 gttcgtcggg tcatgcgtcc caccacatcg tggtggtggc cgcggttgcg gagggtgcaa
      661 aagaaaaggt cccccgatga agagggacct tttcaggtgg agtgccgaag cttacttcac
      721 tttgttgctg aggtcggcac cggctttgaa cttcacgacc ttcttggccg cgatcttgat
      781 ctctttgccg gtctgcgggt tgcggccttt gcgggcggca cgcttggtga tgctgaaggt
      841 gccgaagccg acgagggcca cgcggtcacc ttttttcagc gctttcgtgg tgttgttcac
      901 gaaggcatcc agcgcacgct tggcgtcggc tttggagatc ttcgcttcgg tggcgatcga
      961 ctcgatcagt tcggctttgt tcaggcccat ggttgtggtt ggttggttgt tgttgagttt
     1021 gtaactcggg acgaatcta
//
LOCUS       DAOQOC010001284         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_149718_length_1035_cov_3.781633, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001284 DAOQOC010000000
VERSION     DAOQOC010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149718_length_1035_cov_3.781633"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PL106_16210"
     CDS             <1..617
                     /locus_tag="PL106_16210"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA34675.1"
                     /translation="KEWNEGTIDLSRHPWMQRMHDEGRYAFASDYARFMVLLEHGGLY
                     LDTDVVMKKSFLPFFRERSFWSFEFDSFLSTAIIGAEPGHPLIASLLREYDALAGPRV
                     NNSIVTEYFLAHYPDFHLNNRDQMLPEGVRVYPKEYFVVPGFDQRKNFSTHEANNHWK
                     PEKSGSRAGPVVRRLIGDVLFFKLVNLRMNMTSDYPKMDKARGR"
     gene            complement(636..>1035)
                     /locus_tag="PL106_16215"
     CDS             complement(636..>1035)
                     /locus_tag="PL106_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNA34676.1"
                     /translation="HYRSYKRDHVIRIAGKPELLGSSDTHFRGDAARHNPEELLLAAL
                     SACHMMSYLHVCVTNGVVVTAYTDRPTGVLETVGDGGRMTAVTLHPVVTVLDPSMIPK
                     AEDLHHKAHELCFIAASVNFPVGCEAMVRT"
BASE COUNT          207 a          299 c          326 g          203 t
ORIGIN      
        1 tgaaggagtg gaacgagggg actatcgacc tgtccaggca tccatggatg cagcggatgc
       61 acgatgaagg tcgatatgcc ttcgcgagtg actatgcccg cttcatggta ctgttggaac
      121 acggtggcct gtacctggat acggacgtgg tgatgaagaa gagcttcctg cccttcttcc
      181 gggaacgcag cttctggtcc ttcgaattcg attcgttcct gtccacggcc atcatcggcg
      241 cggagcccgg gcacccgctc atcgcctcgt tgctccgcga gtacgacgcg ctcgccggac
      301 ccagggtgaa caattcgatc gtgaccgagt attttttggc gcactacccg gatttccatc
      361 tcaacaacag ggaccagatg ctcccggaag gggtgagggt gtatccgaag gagtatttcg
      421 tggtgcccgg attcgatcaa cggaagaatt tctccacgca cgaggccaac aaccattgga
      481 agccggaaaa gagcggttca cgggcaggcc cggtggtgcg caggctcatc ggcgacgtgc
      541 tcttcttcaa gctggtgaac ctgcggatga acatgaccag cgattacccg aagatggaca
      601 aggcgcgggg gcgctgatcg atcggccacg tggcttcagg tccgcaccat cgcctcgcaa
      661 cccacgggaa agttcaccga ggccgcgatg aagcacagct catgggcctt gtggtgcagg
      721 tcctctgctt ttgggatcat actcgggtcg agcacggtga caacgggatg cagggtcacg
      781 gctgtcatcc ggccaccgtc accgacggtc tccagcacac ccgtgggcct atcggtgtac
      841 gccgtgacca ccacgccgtt cgtaacgcac acatgcagat agctcatcat gtggcatgcc
      901 gaaagtgcgg ccaggagcag ttcctcgggg ttgtgccgcg cggcatcgcc ccggaaatgc
      961 gtgtcggacg agccgaggag ttccggcttg ccagcgatcc ggatgacgtg gtcgcgcttg
     1021 tagctgcggt aatga
//
LOCUS       DAOQOC010001285         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_149795_length_1035_cov_2.681633, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001285 DAOQOC010000000
VERSION     DAOQOC010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149795_length_1035_cov_2.681633"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..389)
                     /locus_tag="PL106_16220"
     CDS             complement(177..389)
                     /locus_tag="PL106_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34677.1"
                     /translation="MDRIARIMELFWLVLAVLTAGWAVWGLYTSGWNGARHLIWFPLV
                     CTAMFLYRRFTRKKMASWADREKEKG"
     gene            complement(493..>1035)
                     /gene="lptC"
                     /locus_tag="PL106_16225"
     CDS             complement(493..>1035)
                     /gene="lptC"
                     /locus_tag="PL106_16225"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNA34678.1"
                     /translation="RLVLALCALSSCKNDLDELARVDVHAGAPDRTTTDAEYLYSDSG
                     IVRNRLHANKVEDFMAAPGRRTVLTDGMELVFLDAQGNDGSVLTARQGLILPTSNRME
                     VRGNVVFTNARGERLETELLVWNKDSARVWTDRPVKIIREHDILYGDGLDANENFSRY
                     TIRKLTGTLYVDQADSLAGP"
BASE COUNT          176 a          318 c          340 g          201 t
ORIGIN      
        1 gcataatacg gtatagcatg tcgttccgga accggctgat accgccctgt tcggtcggtc
       61 cgccaccacc accggttacc attgctggat cttcaacccg cacggcggct ccgatcagtg
      121 gatcgcgtcc aggtacacga agccgggtat cggccacccc ggtggtgcga gggcactcac
      181 cccttctcct tctcccggtc agcccagctg gccatctttt tccgtgtgaa ccgacgatag
      241 aggaacatgg ccgtgcagac cagggggaac cagatcaggt ggcgggcgcc gttccagccg
      301 ctggtgtaca aaccccaaac agcccagccg gcggtgagga cggccagcac gagccagaag
      361 agttccataa tgcgggcaat gcggtccatg agcggtgcgg tggttatgga tgttgacgga
      421 tcggggatag gaggcgtggc gatggagtga cgggaacaac cgctcattca ggtagcattg
      481 gagcggtcgg tctcatggtc ccgcgaggct gtccgcctgg tccacgtaca gtgttccggt
      541 cagcttgcgg atggtatagc ggctgaagtt ctcgttggcg tcgagtccgt cgccgtagag
      601 gatgtcgtgt tcgcggatga tcttcacggg gcgatcggtc cacacgcggg cgctgtcctt
      661 gttccacacg agcagctcgg tctccaggcg ctcgccgcgc gcgttggtga acaccacgtt
      721 gccgcgcact tccatccggt tgctggtcgg caggatgagg ccctgccggg ccgtgagtac
      781 gctgccatcg ttcccctgtg cgtccaggaa gaccagttcc atcccgtcgg tcagcacggt
      841 gcggcggccg ggtgccgcca tgaaatcctc caccttgttg gcatgcaagc ggttgcgaac
      901 aataccgctg tcgctgtaca ggtattcggc gtcggtggtc gtccggtccg gtgccccggc
      961 atgcacatcc acccgggcca gttcgtccag gtcgttcttg cacgaggaga gcgcacagag
     1021 ggcgagcacg agccg
//
LOCUS       DAOQOC010001286         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_150246_length_1034_cov_1.835546, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001286 DAOQOC010000000
VERSION     DAOQOC010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150246_length_1034_cov_1.835546"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PL106_16230"
     CDS             complement(<1..>1034)
                     /locus_tag="PL106_16230"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA34679.1"
                     /translation="TTPATCGQANGTITSAVTGGTGPLSYLWQPGGATTADLTDLSAG
                     EWTLTATDQSGCAVSASATVADNGSSLAVSVSHTDVTCFGLNNGTAMAAITGSTPASI
                     LWSPLGGDQASATGLAPGDYSVTVTDANGCADTEGLTILEPAPLLVEVDPQVTACPGT
                     TITLTAQTTGGTGAVELHWIPEGPDVSPGTDADFHVSAIDANGCVSDTAMISVIVPGA
                     IIPTLAVDAPSGCAPHCVTLQPGPPGMTYAFTFGDGESGADPVHCYNTPGLYDVVLTA
                     TDDAGCSGTTNFAALLDVLPTPVAGFAIPGTVIISSGPEPVVDASSGATAWRWELDGA
                     HGDSLAAAPVL"
BASE COUNT          196 a          336 c          344 g          158 t
ORIGIN      
        1 agcaccggcg cggcggccag actgtccccg tgcgcaccgt caagctccca acgccaggcc
       61 gttgcccccg aggaagcatc gaccaccggt tccggcccgg aggaaatgat caccgtgccc
      121 ggtatcgcaa agccggccac cggcgttggc agcacatcca gcagggccgc gaagttcgtg
      181 gtgcccgaac agcccgcatc atccgtagcg gtgaggacaa catcatacag gcccggagtg
      241 ttgtagcaat gcacgggatc ggcgccgctc tccccatcgc cgaaggtgaa cgcgtaggtc
      301 atgcccggcg gccccggctg caacgtcacg caatgcggag cgcagcccga gggcgcatcc
      361 acggcgagcg tgggaatgat cgcccctgga acgatcacgg agatcatggc cgtatcggaa
      421 acacagccgt tcgcatcgat ggccgaaaca tggaagtcgg catccgttcc cggggaaaca
      481 tccggacctt caggtatcca atgcagctcc accgcccccg tgccaccggt ggtctgcgcg
      541 gtcagtgtga tggtggtccc cggacaagcg gtaacctgcg gatccacctc caccagcaac
      601 ggtgcgggct ccaggatggt cagcccttcc gtatcggcac agccgttcgc atcggtaacg
      661 gtcacggaat agtccccggg ggcaagtccg gtcgccgaag cttgatcgcc acccaagggc
      721 gaccacagga tcgatgcggg cgtactaccc gtgatcgcgg ccatggccgt tccattgttg
      781 agtccaaagc atgtgacatc ggtatgcgaa accgaaacgg cgagtgatga cccgttgtcg
      841 gccacggtgg ccgaggccga aacagcgcat ccggactgat cggtggcggt gagcgtccat
      901 tcaccggccg acagatcggt gaggtcggcc gtggtggcgc caccgggttg ccacaggtag
      961 ctgaggggcc cggtgccccc ggtgaccgct gaggtgatgg tgccgttggc ctggccacag
     1021 gtcgcggggg tggt
//
LOCUS       DAOQOC010001287         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_150322_length_1033_cov_4.434560, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001287 DAOQOC010000000
VERSION     DAOQOC010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150322_length_1033_cov_4.434560"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PL106_16235"
     CDS             complement(<1..909)
                     /locus_tag="PL106_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34680.1"
                     /translation="MKRYRPLFFSGALAMGLLAHAQPASVLFVGNSYTGTNNLPGMVS
                     QLAASLGTTVSTGMVTPGGYTFLQHSTSPTTLSTIASQQWDYVVLQEQSQLGALPPDY
                     NATAASATVLVDSIRAASECSRPVFYMTWGRQNGDAQFCPSFPYMCTYEGMQQALREN
                     YLAMAEDNDAYTAPVGMAWKQVRDTHPGLQLYVGDGSHPSVQGTYLAACVFYCTLFQQ
                     PCTGASYTGTLSADTAAILQGIASATVLDSMALWNLDLPEGIGAAITGSTSSAWNTVT
                     FHHSGTGTHLWTCSDGQTSTDANPTFT"
BASE COUNT          175 a          299 c          350 g          209 t
ORIGIN      
        1 ggtgaaggtc gggttcgcgt ccgtgctggt ctggccatcg gaacaggtcc acaggtgcgt
       61 gcccgtgccg gagtgatgga aggtgaccgt gttccacgcg ctgctggtgc taccggtgat
      121 cgcggccccg atcccctcgg gcaggtccag gttccacaag gccatgctgt ccaacacggt
      181 ggcgctggcg atgccctgca ggatggcggc cgtatcggct gagagcgtgc ccgtgtacga
      241 tgcgccggtg cagggctgtt ggaagagggt gcagtagaac acgcaggctg ccaggtacgt
      301 gccttgaaca ctcgggtgac taccgtcccc aacatatagt tgcagaccgg ggtgcgtatc
      361 gcgaacctgt ttccaggcca tgcccaccgg ggccgtgtac gcgtcgttgt cctcggccat
      421 ggccaggtag ttctcgcgca gcgcctgctg catgccttcg taggtacaca tgtaggggaa
      481 cgagggacag aactgtgcat cgccgttctg ccgcccccag gtcatgtaga acactggccg
      541 ggaacattcg ctggcggcgc ggatcgaatc caccagcacg gtggccgatg cggcggtggc
      601 gttgtagtcc gggggcaggg cccccaactg gctctgttcc tgcaacacca cgtagtccca
      661 ttgctgcgat gcgatggtgg aaagcgttgt cgggctggtg gagtgctgga ggaaggtgta
      721 gccaccgggg gtcaccatgc ccgtactgac cgtggtgccg agcgaggcgg ccagttgcga
      781 caccatgccg ggcaggttgt tcgtgcccgt gtagctgttg cctacgaaga gcaccgaggc
      841 cggttgtgcg tgcgctaaca gccccatggc gagcgcacct gagaagaaca gtggccgata
      901 tcgtttcatc gttgctgtac cctttgttcc gttgaggatc cgcgttgtcg tgtatgcacc
      961 tcaacagatg ttcacttcgt cccgtattcc ggcgtgaagc ccacactctt gaagaagaac
     1021 gcgtacttgt cgg
//
LOCUS       DAOQOC010001288         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_150404_length_1033_cov_2.739264, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001288 DAOQOC010000000
VERSION     DAOQOC010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150404_length_1033_cov_2.739264"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..>1033
                     /locus_tag="PL106_16240"
     CDS             87..>1033
                     /locus_tag="PL106_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA34681.1"
                     /translation="MAYTRWVAGESRLLLGAGRSLEATHLMDSALVLAEEIEDHNVQL
                     HLLLLRSLAHVQVGHMHQAYADQQAAIAAHDKDMDIEKVRATEKAHSDFQHEKTIALA
                     RAASIRERSQKRAAVAIGSLAFALAFVLYRSFRSKSRYADELHRKNEQIQLAQAQLVA
                     SEKQREAEQVRTRIARDIHDEIGATLTKIRLLSDVALANDADQLSETRHAISRIGQHA
                     RFVSQNMSDIVWAVDPARDTNQGMLDHVRELGHRLLGDNGIAFDLDLSCARPDDLMEP
                     DLRRDMHLVVNEAFNNILKYAHATNVRVRLMLDGPYFELV"
BASE COUNT          226 a          312 c          317 g          178 t
ORIGIN      
        1 aagccctgca caaggccggg aggtgggcgg aagcggactc gatgcaaagg tccacgcgcc
       61 gtttcctccc ggcgctcaag gacccgatgg cgtacaccag atgggtggcc ggtgagtcgc
      121 ggctgctgct cggcgcgggc agatcccttg aagccacgca tttgatggac agtgcgcttg
      181 tattggccga ggagatcgag gaccataacg tgcagctcca tctgctgctc ctgcgatcgc
      241 tcgcacatgt gcaggtcggc cacatgcacc aagcgtacgc ggatcaacaa gcggccatag
      301 cggcgcacga taaggacatg gatatcgaga aggtccgggc cacggagaag gcgcacagtg
      361 acttccagca cgaaaagacc atcgccctgg cacgggccgc atcgatacgt gagcgctccc
      421 agaaacgggc agccgttgcg atcggttcat tggccttcgc actggccttc gtgttgtacc
      481 gttcgttccg ctccaagagc cgatacgccg atgaactgca caggaagaac gagcagatcc
      541 aactcgcgca ggcacaactg gtggccagcg agaagcaacg cgaggccgag caggtgcgca
      601 cgcggatcgc tcgtgacatc cacgacgaga tcggggccac cctcaccaag atccgccttt
      661 taagtgatgt ggcccttgcc aacgatgcgg accagctctc cgagacacgg cacgccattt
      721 cacggatcgg ccaacacgcc agattcgtca gccagaatat gagcgacatc gtgtgggccg
      781 tggatcccgc ccgcgacacg aaccagggaa tgctggacca tgttcgggaa ctcggccacc
      841 gcttgctggg tgacaatggc attgctttcg acctggacct ttcgtgcgcc agaccggatg
      901 atttgatgga accggacctg cgtcgcgata tgcacctggt ggtgaacgag gcattcaaca
      961 acatactgaa gtacgcccat gcgacgaacg ttcgtgtgcg actgatgctc gatggcccgt
     1021 acttcgaact ggt
//
LOCUS       DAOQOC010001289         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_150706_length_1032_cov_2.833163, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001289 DAOQOC010000000
VERSION     DAOQOC010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150706_length_1032_cov_2.833163"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..855
                     /locus_tag="PL106_16245"
     CDS             112..855
                     /locus_tag="PL106_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNA34682.1"
                     /translation="MAKNSSPAPRNEDPSEQRIKKAFKRKGWSEVRANDSWAIFKIMS
                     EFVEGFEAMQRIRPCVSVFGSARTPAEKPEYALAEEIAFQLTQHGYGVITGGGPGIME
                     AANKGAQRGGGTSVGLNIELPFEQEPNAFIDKEKSLHFDYFFVRKVMFVKYSQGFIGL
                     PGGFGTLDELFEAVTLIQTQKIARFPIILVSRKYWAGMFEWIKRTMENEYHYIGPNDL
                     DLIQLVDSAEEAVDAIDKFYSRYMLKPNF"
BASE COUNT          224 a          305 c          318 g          185 t
ORIGIN      
        1 ctgaactgcg cacggggcgg tggaaaagtg ggggaccggt ccacatgccg aacggaccgg
       61 caccagtaga cttgcgatcc atcgatcccc tctccgagca tccgcaaccc catggccaag
      121 aacagcagcc ccgcgccgcg taacgaagac ccgagcgaac agcgcatcaa gaaagccttc
      181 aagcgcaagg gctggagcga ggtgcgcgcg aacgacagct gggcgatctt caagatcatg
      241 agcgagttcg tggaaggctt cgaggccatg cagcggatcc gtccatgcgt cagcgtattc
      301 ggcagcgcgc ggacccctgc ggaaaagcct gagtacgcgc tggcggaaga gatcgcattc
      361 cagctgacgc aacatggtta tggcgtgatc accggcggtg gccccggcat catggaagcg
      421 gccaacaaag gagcgcaacg gggtggcggc accagtgtgg gcctcaacat cgaattgccc
      481 tttgaacagg agccgaacgc gttcatcgac aaggagaaga gcctgcactt cgattacttc
      541 ttcgtacgca aggtgatgtt cgtcaagtac agccagggct tcatcggcct gcccggtggc
      601 ttcggaacgc tggacgagct cttcgaggcc gtcaccctga tccaaacgca gaagatcgcg
      661 cggttcccca tcatcctggt aagcaggaaa tactgggccg gcatgttcga gtggatcaag
      721 cggaccatgg agaatgagta tcattacatc ggcccgaacg atctggacct gatccaactg
      781 gtggacagcg ccgaggaggc cgtggacgcc atcgacaagt tctacagccg ttacatgctg
      841 aagccgaact tctgagtctc ggctgtccgg atggatgccg gatccggtga tttttcctgc
      901 taagggccgt atgccctgta tcgtccgcac ctccgttgtc ggatgttggt cgctgagcgc
      961 tgacctcccg atcatcgcgg ccgatccacg ctgaaggtgc acgtgtttcg gctcgaagtc
     1021 cgcaccttcg cg
//
LOCUS       DAOQOC010001290         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_151245_length_1030_cov_3.624615, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001290 DAOQOC010000000
VERSION     DAOQOC010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151245_length_1030_cov_3.624615"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..930
                     /locus_tag="PL106_16250"
     CDS             49..930
                     /locus_tag="PL106_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA34683.1"
                     /translation="MPTPSTTADRIAPKAAPAAHAGHFNVYEIEPFHREPKGPLPYDK
                     RDFYKVTVLEGASRMMYADREVQVDKQALVFSNPLEPYCWEHFKSVHQGHYAIFNDAF
                     FPEYGSLARYPVFQPYHDHVFGLDDEQLAHVKDLFRRMHAEMRSDYAFKYDLLRSLCQ
                     ELMHFGMQLRPLVHEKSAPNANTRLAHLFIELLGRQFPINEMHPRMELRTASDFADAL
                     SVHTNHLNRALKEVMGHTTTGLIADRVLHESKMLLGRTTWSVAQIADALGFTEPAHFS
                     NFFRKHTAQSPMQFRAV"
BASE COUNT          198 a          342 c          306 g          184 t
ORIGIN      
        1 cgttggcccg aaatgcttgt ccaaagccgg gtgtcgtatt ttcaccccat gccaaccccc
       61 agcaccaccg ccgatcgcat cgccccgaaa gcggcgcccg ccgcccatgc cgggcacttc
      121 aacgtgtacg agatcgagcc cttccaccgg gaaccgaagg ggccgctgcc ctacgacaag
      181 cgcgacttct acaaggtgac cgtgctggag ggcgccagcc gcatgatgta cgccgaccgg
      241 gaggtgcagg tcgacaagca ggccctggtg ttcagcaatc ccctggaacc ctattgctgg
      301 gagcatttca agagcgtaca ccagggccac tacgccatct tcaacgatgc gttcttcccg
      361 gaatacggtt cgcttgcacg ctatccggtc ttccagccgt accatgacca tgtgttcgga
      421 ttggacgatg aacaattggc gcacgtgaag gacctcttcc ggcgcatgca cgcggagatg
      481 cgctcggact acgccttcaa gtacgacctg ctgcgttccc tttgccagga gctgatgcac
      541 ttcggcatgc agctgcgccc gctggtgcat gagaagtcgg ccccgaacgc caacacgcgc
      601 ctggcgcacc tgttcatcga gctgctgggc cggcagttcc ccatcaatga gatgcacccg
      661 cgcatggagc tgcgcacggc ctcggatttc gccgatgccc tgagcgtgca taccaaccac
      721 ctgaaccgcg cgctgaagga agtgatgggc cataccacca ccggcctgat cgccgatcgc
      781 gtgctgcacg aatcgaagat gctgctgggc cgcaccacct ggtcggtggc gcagatcgcc
      841 gatgcgctgg gcttcacgga acccgcccac ttcagcaact tcttccgcaa gcacacggcc
      901 cagagcccga tgcagttccg ggctgtttga taggtgcaag ggcggaccgg ccacgcaggc
      961 ggtacgcgtt acggagcgac gggggttcgc acccacatcg gtttggtgga tcaggatgag
     1021 gtcagctgtt
//
LOCUS       DAOQOC010001291         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_151895_length_1028_cov_3.063720, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001291 DAOQOC010000000
VERSION     DAOQOC010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151895_length_1028_cov_3.063720"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PL106_16255"
     CDS             <1..205
                     /locus_tag="PL106_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA34684.1"
                     /translation="KLLDYDAITPEVLPADKSGALVFYCWSPECPAAGMAAESAVKLG
                     YTDVRCMHAGITGWQDAGLPTEP"
     gene            202..636
                     /locus_tag="PL106_16260"
     CDS             202..636
                     /locus_tag="PL106_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34685.1"
                     /translation="MRTTELVLIVLIVLGCIFKIQHWPGANAFILVGGGTLALFYFPF
                     GFRTLAAPRSTDQILWFTLLGGLALNAALSGLLAFQLRWPYNKELLVIGAIGCAVTLL
                     SGVVLRYKHPRMDIYLEGMLIRCLVLGGLAFMLWGLFAGKPR"
     gene            complement(643..>1028)
                     /locus_tag="PL106_16265"
     CDS             complement(643..>1028)
                     /locus_tag="PL106_16265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA34686.1"
                     /translation="VRMREFGASVLEPKEITYRFDAQGDHSTALPPMVKSNLYLIFKE
                     AVNNAAKHAQATAVSVAISYRNNRLKMTIADNGRGLPDSNGASRAHGGNGLRNMRKRA
                     EEMNAELHMDSTPEKGTTIDLVIPL"
BASE COUNT          162 a          324 c          312 g          230 t
ORIGIN      
        1 gaagctcctg gactacgacg ccatcacacc agaagttctt ccggctgaca agtcgggcgc
       61 gctggtgttc tactgctgga gcccggaatg ccccgctgcc ggcatggccg cggaaagcgc
      121 cgtgaagctg ggctacaccg atgtgcggtg catgcacgcg ggcatcaccg gctggcagga
      181 tgcgggcttg cccaccgagc catgaggaca acggagctcg tgctgatcgt cctgatcgtc
      241 ctgggctgca tcttcaagat ccagcactgg cccggggcga atgcgttcat ccttgttgga
      301 gggggcacgc ttgcgctctt ctattttccg ttcggcttcc gcacactggc agcacccagg
      361 tcaacggacc agatcctgtg gttcaccctt cttggaggcc tcgcattgaa cgcggccttg
      421 tcgggactgt tggccttcca actccgatgg ccctataaca aggaattgct ggtcatcggc
      481 gcgatcggtt gtgcggtcac cttgttgagc ggcgtggttc ttcggtacaa gcatccacgc
      541 atggacatct acctcgaggg tatgctgatc cgatgtctgg tgctgggcgg tctggcattc
      601 atgctctggg gccttttcgc gggcaagccg cgctgatacc gatcacaggg ggatcaccag
      661 atcgatcgtg gtgcccttct ccggtgtgct gtccatgtgc agctccgcgt tcatttcctc
      721 ggcgcgcttg cgcatgttcc gcaggccgtt gcccccatgc gcgcgcgatg caccgttcga
      781 atccggcaaa cccctgccgt tgtcggcgat ggtcatcttc aaccggttgt tccggtagct
      841 gatggccacg ctgaccgccg tggcctgggc atgcttcgcc gcgttgttca ccgcttcctt
      901 gaagatgagg taaaggttgc tcttcaccat cggcggcaaa gccgtgctat gatcgccctg
      961 cgcatcgaag cggtaggtga tctccttcgg ctccagcacc gacgccccga actcgcgcat
     1021 gcgcacca
//
LOCUS       DAOQOC010001292         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_154129_length_1021_cov_3.609731, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001292 DAOQOC010000000
VERSION     DAOQOC010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154129_length_1021_cov_3.609731"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PL106_16270"
     CDS             complement(<1..353)
                     /locus_tag="PL106_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34687.1"
                     /translation="MTASLRLPSNLLLITALALLAQRGYGQWVVLGSPLSTATSVNNI
                     TGGGMMVTEYVQPPSPCAQGSARFAVLDPSTPFGSTDYGGASAYISVTPAGQLLQQFF
                     VNISFEDQTGLPNNV"
     gene            complement(469..>1021)
                     /locus_tag="PL106_16275"
     CDS             complement(469..>1021)
                     /locus_tag="PL106_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA34688.1"
                     /translation="EMQGGTIAVRSEAGKGSTFTATIPYRLNERAVPSERPEDAAHPA
                     GSVGHRALRVLLVEDHHFNVMVAQAELAQVLPKAQVDVAGNGERAVEMVKNGHYDVVL
                     MDVQMPVMDGYAATRAIRALVGDRSRVPIIAMTANVMEAEVDRCREAGMNGFIPKPFQ
                     QAELATALANELATVDRSSPRTS"
BASE COUNT          195 a          328 c          304 g          194 t
ORIGIN      
        1 caaacattgt tcggtagacc ggtctggtcc tcgaatgaaa tgttgacgaa gaactgctgc
       61 aacaactggc ccgcaggtgt tacggaaatg taggcgctcg cccccccgta gtccgtggag
      121 ccgaacggtg tactgggatc gagcaccgcg aaacgggcgc tgccctgggc acaaggagag
      181 ggcggttgca catactccgt gaccatcatg cccccgccag tgatgttgtt cacggaggtg
      241 gctgtactga gcggactacc gagtacaacc cactgcccat atcccctttg cgcgagcaga
      301 gccagggcgg tgatcaggag caaattgctg ggaagtcgta aggatgcggt catgttgttg
      361 gagggttggt gcggacggtg aacgcgaaga cattcatgga ccgcacaaaa ctccttcggg
      421 cgcacggcgt ggcagcccgt gggaacaccg gttcttgggc tggtagaact acgaggtgcg
      481 cggtgatgac cgatccacgg tcgccagctc gttcgccagc gccgtggcca actccgcctg
      541 ctggaacggc ttgggtatga acccgttcat tcccgcctcg cgacagcgat ccacctcagc
      601 ctccatcacg ttcgctgtca tggcgatgat gggcacgcgc gaccggtcac cgaccagggc
      661 acggatcgcc cgcgtggccg cgtaaccgtc catcacgggc atctgcacgt ccatgagcac
      721 cacatcgtag tgcccgttct tcaccatctc caccgcacgt tcaccgttcc cggccacatc
      781 cacctgcgct ttcggaagca cctgcgccag ctcggcctgt gccaccatca cgttgaagtg
      841 atgatcctcc accagcagca cacgaagtgc tcggtgacca acggatcctg ctggatgcgc
      901 ggcatcctct ggtcgttcac tcggcaccgc ccgctcgttg agacgatagg gtatcgtggc
      961 tgtgaaagtg ctgcccttcc cagcctcgct gcggacagcg atggtgccac cctgcatctc
     1021 c
//
LOCUS       DAOQOC010001293         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_154457_length_1020_cov_4.167876, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001293 DAOQOC010000000
VERSION     DAOQOC010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154457_length_1020_cov_4.167876"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PL106_16280"
     CDS             complement(<1..836)
                     /locus_tag="PL106_16280"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34689.1"
                     /translation="MRQRRYYLLSALLTGLLLALSWPFIGSATPLVFFAWVPLLLAEE
                     RYSTRTRQQRPRHFMPYVFLAMAIWNALTTWWLYCVSESMGSKLFAVVGPNVGNDLLF
                     CLPWLVMRWSRWFVPSRWVRLVLVVTWIAFERFHMNWDLSWPWLTLGNVFAQHPAWVQ
                     WYEWTGHLGGSLWVWTANLAVLAVVKNPDGHVPGRRSLIAALVILAPFIASQVRYVTF
                     KETGTPVEVVVVQPDIDPYTEKFDEDPIVQLDHMLEQASAALTDSTVLVVLPETALQE
                     QPR"
BASE COUNT          221 a          305 c          330 g          164 t
ORIGIN      
        1 cggggttgtt cctgcaaggc ggtttcgggc aacaccacga gcaccgtgga atcggtaagc
       61 gcagcgctgg cctgctccag catgtgatcc agttggacga tcgggtcctc gtcgaatttc
      121 tcggtgtacg gatcgatgtc gggctgtacc accaccactt ccaccggcgt acctgtttcc
      181 ttgaatgtga catagcgcac ctgcgaggcg atgaagggtg ccaggatcac cagtgccgcg
      241 atgagcgacc gtcggcccgg tacatgtcca tcagggttct tcaccaccgc cagtacggcc
      301 aggttcgcgg tccacaccca gagcgatcca ccaaggtgcc cggtccattc gtaccactgc
      361 acccaggcgg gatgctgggc gaagacattg cccaaggtga gccacggcca gctgaggtcc
      421 caattcatgt ggaagcgctc gaaggcgatc caggtcacca ccagcacgag gcgcacccag
      481 cggctgggca cgaaccatcg gctccagcgc atcaccagcc agggcaggca gaagagcagg
      541 tcgttgccca cgttcgggcc gacgacggcg aagagcttgg agcccatcga ctcgctcacg
      601 cagtacagcc accacgtggt cagggcgttc cagatggcca tggccaagaa cacatacggc
      661 atgaagtggc gcggtcgctg ctgccgtgtc ctcgtggaat agcgttcctc ggccagcagc
      721 aaggggaccc aagcaaagaa taccagcggt gtggcgctac cgatgaatgg ccagctcaat
      781 gccaacagca gcccggtgag cagggcggag aggaggtagt agcggcgttg gcgcatcagg
      841 gcttcgggcg gggcggcgca aagtagctgt ggaccagtga gcttccggag tagtgcgcag
      901 gacattcaac ggggctgggc catccgtgtc catccgaggt agaacgcagc acgtcctccg
      961 aaggtggacg aacgaagaac gtatgtggag gaaaaatgac aagcccccca aattgcttgg
//
LOCUS       DAOQOC010001294         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_154501_length_1020_cov_3.197927, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001294 DAOQOC010000000
VERSION     DAOQOC010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154501_length_1020_cov_3.197927"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PL106_16285"
     CDS             <1..972
                     /locus_tag="PL106_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34690.1"
                     /translation="ACTIVPVVSPEALVLCPNSADQLSTGTFDSYQWYKNGAEIPGAT
                     AQTLPVTSADDAGAWFKVRAANDTCFGFSDSVLVDGYVFPPPTISQAGDTPNLIGDEG
                     EQIYCQGDAPILVLDAPYDTNVQWYSAGEPIVGAVGTALGVSTSGSYSVHGAPATCPN
                     YIQDAGVAIVMEFHPNVQPVIVQSGDLLCPEPEGISAQWYFNGQPIAGIEPCTFPVGP
                     GAYTVFVDYGDSCSVLSGPFTVVGMEERARSILNAWPVPTGGSITISGDPSVPLPAWD
                     LLDACGRVVLHGPGGRAPLLIDLGALENGHYRFRMQDGRALFVAVAH"
BASE COUNT          180 a          313 c          346 g          181 t
ORIGIN      
        1 gcctgcacga tcgtgcctgt ggtgtcgccc gaagcgctcg tgctgtgccc caacagcgcg
       61 gaccagcttt caacaggcac cttcgacagc taccaatggt acaagaacgg ggcggagatc
      121 ccgggggcca cggcgcaaac gcttcccgtt acctcggccg atgacgcagg ggcatggttc
      181 aaggtgcggg ccgcgaatga tacctgcttc ggtttctcgg acagcgtact ggtggacggg
      241 tacgtgttcc cgcccccaac gatcagccag gcgggcgaca ccccgaacct gatcggcgat
      301 gagggcgagc agatctattg ccagggcgat gcgccgatcc tggtactgga tgcgccatac
      361 gataccaacg ttcaatggta ctccgccggg gagcccatcg ttggcgccgt tggcaccgcc
      421 ttgggcgtga gcacgtcggg cagctacagc gtacatggcg cgccagcgac ctgcccgaac
      481 tacatccagg atgcgggcgt tgctatcgtg atggagttcc atcccaacgt gcaaccggtc
      541 atcgttcaat ccggcgacct gctctgcccc gagccggagg gcatcagtgc gcagtggtac
      601 ttcaacggcc agccgatcgc ggggatcgaa ccgtgcacgt tcccggtggg tccgggcgcg
      661 tacacggtgt tcgtggacta cggggacagt tgctcggtgc tttcggggcc cttcaccgtg
      721 gtcggcatgg aggagcgggc ccgaagcatc ttgaacgcct ggccggtacc cacgggagga
      781 tcgatcacca tcagcgggga tccgtccgtt ccgttgccag catgggacct gctggatgcc
      841 tgcggccgcg tggtgctgca tggccccggt ggacgggcac cgctgctgat cgacctgggc
      901 gcattggaga acgggcacta ccgtttccgg atgcaggatg ggcgggcgct cttcgtggcg
      961 gtggcgcact gagaccgaag atgtctgaaa ccgcaaagag gcggtaatac cgattggaca
//
LOCUS       DAOQOC010001295         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_155170_length_1018_cov_4.471443, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001295 DAOQOC010000000
VERSION     DAOQOC010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155170_length_1018_cov_4.471443"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..598)
                     /locus_tag="PL106_16290"
     CDS             complement(32..598)
                     /locus_tag="PL106_16290"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA34691.1"
                     /translation="MRRYNQRLYRAIRGYLPDGADVQDAMQETYVKAFKNLDRYNGDA
                     AFSTWLIRIGINEALQHLRRTKRRTAHMVLDTDQELTHELPDTHMDPEHTVEHAETRR
                     IVERAVDQLPEAYRAVYLLREVEELPSAEVAACLGISESNVKVRLHRAKALLRETVWE
                     QMEDAPVFEFGQDHCDALVQRVMERIGG"
     gene            809..>1018
                     /locus_tag="PL106_16295"
     CDS             809..>1018
                     /locus_tag="PL106_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34692.1"
                     /translation="MNIAINQEATRLQTLLKFTYGLVPIAAGADKFTNILTDWSGYLA
                     PGLAGMLPFDPATFMMVVGVIEIVAG"
BASE COUNT          164 a          342 c          303 g          209 t
ORIGIN      
        1 ccgccggggg cgccctgctt cgccaggttg ttcagccacc gatccgctcc atcacccgct
       61 gcaccagcgc atcgcaatgg tcctgcccga actcgaacac cggcgcgtcc tccatctgct
      121 cccacaccgt ttcgcgcagc agcgccttgg cccggtgcag gcgcactttc acgttgctct
      181 cgctgatccc caggcaggcg gccacctcgg cgctgggcag ttcctccacc tcgcgcagca
      241 ggtacaccgc gcggtacgcc tcgggcaact ggtccaccgc acgttccacg atgcggcgcg
      301 tctcggcatg ttcaacggta tgctcggggt ccatgtgcgt gtcgggtagc tcgtgggtca
      361 gttcctggtc agtgtccagc accatgtggg cggtccgcct cttggtgcgg cgcaagtgtt
      421 gcagcgcctc gttgatgccg atgcggatga gccaggtgga aaaggccgcg tcgccgttgt
      481 accggtcgag gttcttgaac gccttcacgt aggtctcctg catggcgtcc tgcacatcgg
      541 ccccgtcggg caagtagccg cgaatggccc ggtagagccg ctggttgtac cgtcgcatca
      601 ggatctccta caggcccgtg cggcccgcga tcacttccgc gatcacctcc aggtcggaca
      661 ggggcgttcc gggctttggt gcgctatgca atgccttcat gtgcgtgacg ttccacctgt
      721 ttgatgcatc tcgacggtga aagttacacg gtcttgtaac cgatggtcag ttgtggcatc
      781 caacgagtgt aaccaacaac cgcccaacat gaatatcgcg atcaaccagg aagcaacacg
      841 cctccagacc ctgctcaagt tcacctacgg ccttgtgccc atcgccgcag gtgccgacaa
      901 gttcaccaac atcctcaccg actggagcgg ctacctcgcg cccggtctgg ccggtatgct
      961 gccgttcgat ccagcgacct tcatgatggt ggtgggtgtg atcgagatcg tggcgggt
//
LOCUS       DAOQOC010001296         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_155286_length_1018_cov_2.480789, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001296 DAOQOC010000000
VERSION     DAOQOC010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155286_length_1018_cov_2.480789"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..520)
                     /locus_tag="PL106_16300"
     CDS             complement(206..520)
                     /locus_tag="PL106_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34693.1"
                     /translation="MPTNGSNKAIEQMKLPVNRMWSGKIRVDVGMTSRTPQERFQQHR
                     TGYRNAKGLKLSSEIVRKYGRYHRPSLYRDIGPFSRAEALEEEKGLTLELRRKGYAVW
                     SN"
     gene            549..>1018
                     /locus_tag="PL106_16305"
     CDS             549..>1018
                     /locus_tag="PL106_16305"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNA34694.1"
                     /translation="MDARTQEPLPYASVVTSKNGQGVITNEEGIFRITAVAEEDTLVF
                     SYLGYRSVSMSAMQVRSLRDVRLQPSTTELSAVQVVGRNDALYDLVIAAGKHLRNAAR
                     YEGKTYYQLETRTLDLPVEGVECFYNGRFNGADIEALDLKQGRIGLLPVDGRYMV"
BASE COUNT          188 a          296 c          319 g          215 t
ORIGIN      
        1 ggccgtcagg cttccgcaga gacttctgga tgatcctttc acgagaattt ccagggaccg
       61 ccacaggcgg agaggagcca tggcactcgg gaaacatccg agcgccggtc acatgggtgc
      121 agagtcgcgc gagtgtgggc cgatacggag caggccgatg gtgtacatcc tcaggtaaaa
      181 gcctcgcgcc gtgggtgggc acacctcagt tggaccacac cgcgtacccc ttccgccgaa
      241 gttcaagcgt cagtcctttc tcctcctcga gcgcttccgc gcgactgaac ggtccgatgt
      301 cccggtacag gctcggccgg tggtagcggc cgtacttgcg cacgatctcc gaagagagct
      361 tgagtccttt ggcgttccgg tatccggtcc tgtgctgctg gaaacgttcc tgtggcgtcc
      421 tgctcgtcat gcccacatcg acccgtattt tcccgctcca catgcggttc accggaagtt
      481 tcatttgctc gatcgccttg ttgctcccat tggtgggcat tggtcaggct acgctgcaag
      541 gtcgcgtgat ggatgcccga acgcaggagc cactgccgta tgccagcgtg gtcacctcaa
      601 aaaatggcca gggcgttatc acaaacgaag agggcatctt ccgcataaca gccgtagcag
      661 aggaggatac gttggtgttc agctatctgg gctaccggtc ggttagcatg tccgcgatgc
      721 aggtgcgctc cctgcgtgat gtccgcttgc agccttccac cacggagctt tccgctgtgc
      781 aggtggtagg ccgcaacgat gcgctctatg acctggtgat cgccgctgga aagcatttgc
      841 gcaatgccgc gcgatacgag gggaagacgt actaccagtt ggaaacgcgc acccttgatc
      901 ttccggtgga aggagtggag tgcttctaca acggccgctt caacggggcg gacatcgagg
      961 cgttggacct gaagcagggg cgcatcggtc tgttgccggt agatgggcgc tacatggt
//
LOCUS       DAOQOC010001297         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_155495_length_1017_cov_4.178794, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001297 DAOQOC010000000
VERSION     DAOQOC010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155495_length_1017_cov_4.178794"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1017)
                     /locus_tag="PL106_16310"
     CDS             complement(<1..>1017)
                     /locus_tag="PL106_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNA34695.1"
                     /translation="EKIDMLDFADVVAINKFDKRGAQDALRDVKKQYKRNHQLWDAKD
                     EDLPVVGTIASQFNDPGTNTLYARLMKKIAEKTGIALDSAFHAHDEMSEKVWIIPPAK
                     SRYLSEISESNRRYDARVQAQAAIADRLYGLHSAVLTFGGPDLLVGEWSLVNGEWKNA
                     GDASSPVAFHHSPITSTPELETLIRKFHEHRKDLDPHLWSMLTGWKSEEARYSGEFYT
                     YSVRGKEIKVPNHSESLSHLKIPKVALPKFRSWGDKVRWAMQENTPGFFPYTAGIYPF
                     KRTGEDPARMFAGEGGPERTNKRFHYVSQGLPAKRLSTAFDSVTLYGHDPGRRPDIYG
                     KVGN"
BASE COUNT          158 a          312 c          319 g          228 t
ORIGIN      
        1 tgttgcccac cttgccgtag atgtccggac ggcgaccggg atcgtggcca tagagggtca
       61 cgctatcgaa ggccgtgctg aggcgcttgg cgggcaggcc ctggctcaca tagtggaagc
      121 gcttgttggt gcgctcgggg ccgccctcgc cggcgaacat ccgcgcgggg tcctcgccag
      181 tgcgcttgaa gggatagatg cccgcggtgt aggggaagaa gccgggcgtg ttctcctgca
      241 tggcccagcg caccttgtcg ccccaactgc ggaacttcgg cagcgccacc ttggggatct
      301 tcaaatggct caggctctcg ctgtggttgg gcaccttgat ctccttgccg cgcacactgt
      361 acgtatagaa ctccccgctg tagcgtgctt cctcgctctt ccagccggtg agcatgctcc
      421 acaaatgcgg gtcgaggtct ttgcggtgtt cgtggaattt ccggataagg gtttcgagtt
      481 ccggcgtgga ggtgattggt gaatggtgaa aagcgactgg ggagctcgcg tccccggcat
      541 tcttccattc gccattcacc agtgaccatt cacccaccag cagatccgga cctccgaacg
      601 tgagcactgc gctgtgaagt ccgtacaaac ggtcggcgat ggcagcctgc gcctgcaccc
      661 ttgcatcata cctccggttg ctctcgctga tctcactcag gtaacggctc ttcgcgggcg
      721 ggatgatcca caccttctcg ctcatctcgt cgtgcgcgtg gaaggcggag tcgagcgcga
      781 tgcccgtctt ctccgcgatc ttcttcatca agcgcgcata gagcgtgttg gtgccgggat
      841 cgttgaactg gctggcgatg gtgcccacca cgggcaggtc ctcgtccttg gcgtcccaca
      901 gctggtgatt gcgcttgtac tgcttcttca cgtcgcgcag ggcgtcctgg gcaccgcgct
      961 tgtcgaactt gttgatggcc acgacgtcgg cgaagtcgag catgtcgatc ttctcca
//
LOCUS       DAOQOC010001298         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_155522_length_1017_cov_3.393971, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001298 DAOQOC010000000
VERSION     DAOQOC010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155522_length_1017_cov_3.393971"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1017)
                     /locus_tag="PL106_16315"
     CDS             complement(<1..>1017)
                     /locus_tag="PL106_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNA34696.1"
                     /translation="EQRGITVVPEIEFPGHCSAALAAYPQLGCRKDTIAVPTTWGVFH
                     DVYCVGQEETWSFFQSVLDELLPLFPAPWFHIGGDEVPKDRWQHCAVCQARMKNEGLK
                     DEHALQAWAVKRLQAYLTGKGKRLIGWDEVLEGGLGSTAVVEVWRGDEQARKARANGN
                     AMIRTLYFDASPASLTLDKVKAFDPLVDGDAHGILGAECPLWSETADERSVGYRIFPR
                     LHVFAERMWSGEVRGDIMERTAPHVARLEQEGWVTATTDKDLFRSAAVFDPQKRDWLI
                     TAERGRPDMDVAWSSEHGSGTFTDSLRVVAPGTVKLTPRWREKPLTDATSIRIAPHLA
                     LGAR"
BASE COUNT          173 a          336 c          313 g          195 t
ORIGIN      
        1 gtcgcgcacc cagggccagg tgcggtgcga tgcggatgct ggtggcatcg gtcaatggtt
       61 tctccctcca acgcggcgtg agcttcacag tgcccggtgc aactacacgc agggagtcgg
      121 tgaacgtccc ggatccgtgt tccgatgacc aggccacatc catatcaggt cgtccgcgtt
      181 ccgcagtgat cagccagtcg cgtttctgcg gatcgaagac cgctgcactg cggaaaagat
      241 ccttgtccgt cgtggccgtg acccatcctt cttgctccag ccgtgcgaca tggggtgcgg
      301 tgcgttccat gatatcaccg cgcacttcac cgctccacat ccgctcagcg aatacgtgca
      361 agcgcgggaa gatgcggtag cccacgctgc gttcatcggc ggtctcgctc cacaaggggc
      421 actcggcacc gaggatgccg tgcgcatcgc cgtccaccaa cggatcgaag gccttcacct
      481 tgtccagcgt gaggctggcg ggtgaggcat caaagtagag cgtgcggatc atggcgttgc
      541 cgttcgcacg ggccttgcgt gcctgttcat cgccgcgcca cacttccacg acggccgtgc
      601 tgcccagccc accttcgagc acctcatccc aaccgatcag ccgtttgccc ttgccggtga
      661 ggtaggcctg caagcgcttc accgcccagg cttgcagcgc gtgttcatcc ttcaagcctt
      721 cgttcttcat cctcgcttga cagaccgcgc aatgctgcca gcggtccttg ggcacctcgt
      781 cgccgccgat gtggaaccac ggcgcgggga agagcggcag gagttcgtcg agcacgcttt
      841 ggaagaaact ccaggtttcc tcctggccca cgcaatacac atcgtggaaa acgccccagg
      901 tggtgggcac ggcgatggtg tccttgcggc aaccgagctg cggataggcc gcgagcgccg
      961 ccgagcaatg gccggggaat tcgatctcgg gcaccacggt gatcccgcgt tgttcgg
//
LOCUS       DAOQOC010001299         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_157563_length_1011_cov_2.900628, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001299 DAOQOC010000000
VERSION     DAOQOC010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157563_length_1011_cov_2.900628"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..>1011)
                     /locus_tag="PL106_16320"
     CDS             complement(34..>1011)
                     /locus_tag="PL106_16320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNA34697.1"
                     /translation="STMRRNLPVVSSDTVVAGAFTHYVDVHGNVRADKAAALYSNGGR
                     VRRILVQPGDAVRRGDLIISIDNDMAAEQIQQAEAAYELAKTAFEKQERLWKQKIGSE
                     MQYLQAKSQKEQAEAGLATLREQQRLTNITAPFDGTVDDIMVRVGDMTSPLQPAARVV
                     DLSGVQLEADVPESYLATIRNGAPVKVSFPSLDVHFDATLAHVGKFIDPSNRTFKVTV
                     RVPQGEPSMRPNLLSDISIQDHHSDSALVVPGRAVLQDVKGNNYIFTLEPTRGNEAVC
                     HKVMVDRLSGYKGRVSIAPVTPGALKGGERIVDEGAKNVTDGLTVRVAN"
BASE COUNT          154 a          321 c          315 g          221 t
ORIGIN      
        1 gtgtcctctg tgcctctgtg gtgaggatgt tcctcaattc gccacgcgca cagtcaggcc
       61 atcggtgaca ttcttggcgc cttcgtccac gatccgttct ccgcccttca gtgcaccggg
      121 ggtcactggt gcaatgctga cgcggccttt gtatccactc aggcgatcga ccatcacctt
      181 gtggcagacg gcctcattgc cgcgggtcgg ttccaacgtg aagatgtaat tgttgccctt
      241 cacatcctgg agcaccgcgc gcccgggcac caccagcgca ctgtcgctgt gatggtcctg
      301 gatgctgatg tcactcagca ggttggggcg catgctcggt tcgccctggg gtacgcgcac
      361 cgtcaccttg aacgtacggt tcgaaggatc gatgaacttg cccacatgcg ccagggtggc
      421 atcgaaatgc acatcaaggc tcgggaagct caccttcacg ggtgcgccgt tccggatggt
      481 ggcgaggtag ctctcgggca catcagcctc cagctgcaca ccgctcaggt ccaccacgcg
      541 cgcggcgggc tgcagcggac tggtcatgtc gccaacacgc accatgatgt cgtccaccgt
      601 gccgtcgaaa ggcgcggtga tgttggtgag gcgctgttgt tcgcgcaggg tggcgaggcc
      661 cgcttcggcc tgttccttct ggcttttggc ctggaggtat tgcatctcgc tgccgatctt
      721 ttgcttccac aggcgctcct gcttctcgaa ggcggtcttc gccagttcgt aggcggcctc
      781 ggcctgttgg atctgttcag cggccatgtc gttgtcgatc gagatgatca ggtcgccgcg
      841 ccgcaccgca tcacctggtt gcaccaggat ccggcgcacg cgcccgccgt tgctatagag
      901 tgcggcggcc ttgtcggcgc gcacgttgcc gtgcacgtcc acgtaatggg tgaaagctcc
      961 cgccaccacg gtatcggaag agaccaccgg caggttgcga cgcatggtgc t
//
LOCUS       DAOQOC010001300         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_157924_length_1010_cov_2.544503, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001300 DAOQOC010000000
VERSION     DAOQOC010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157924_length_1010_cov_2.544503"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1010)
                     /locus_tag="PL106_16325"
     CDS             complement(<1..>1010)
                     /locus_tag="PL106_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34698.1"
                     /translation="LPGTPCEGASVTLNNTSGGVFDWEWLVDGSGVPNNTVEDLVYTF
                     PSTTTFTVSLIASTQTSPACPDTATMTITPVEGPSVAITTLDAATCGSTISVDLQADA
                     TGNNLAFNWQWSGGSAGIEDPGDQVFTMNGGVLTTYTVSVGVTSSGSTCAEVTDQITV
                     TLYPEPVAGLVVGPAPCVGQPYVFTSSSIGADVIGWTVDGTVQPTGPDLTHTFATGGP
                     HTVGIFASTNTVPACIDSSDLVVQVAEQPTVDLPLTAIDTCAAELLLDFAPVFSGTDP
                     SFSWDFGTSADPDNIISPAPTNVLFPGDAFAPITYPVVVSVSVPNSVCPSVSDATTVT
                     LL"
BASE COUNT          204 a          288 c          338 g          180 t
ORIGIN      
        1 ggagcagggt caccgtggtc gcatcggaaa cggaggggca gacgctgttc ggaacggaga
       61 cggagaccac gaccgggtat gtgatcggag cgaacgcgtc ccccggaaaa agcacgttgg
      121 tgggtgcggg cgaaatgata ttgtcgggat cggccgaagt accgaaatcc caggagaatg
      181 atggatccgt cccgctgaac accggtgcga aatccagcaa cagttccgcg gcgcaggtgt
      241 cgatggctgt gagcggcagg tccacggttg gttgttcggc cacttggacc accagatcgc
      301 tggaatcgat gcaggcgggg accgtgttgg tggaagcgaa gatccccacc gtgtgcggcc
      361 ccccagtggc gaaggtgtgg gtcaggtcag ggcccgtcgg ctgtaccgtc ccgtccacgg
      421 tccaaccgat gacgtcggcc ccgatgctgg agctcgtgaa cacgtatggt tgcccaacgc
      481 atggtgccgg tccgacaaca agaccggcca ccggttcggg ataaagggtg acggtgatct
      541 gatcggtcac ctccgcacaa gtacttcccg aactggtcac acccaccgag accgtatagg
      601 tggtaagtac gccaccgttc atggtgaata cctgatcacc aggatcctcg atcccggcgg
      661 atccaccgct ccattgccag ttgaaggcga ggttgttccc tgtggcatcg gcctgcaggt
      721 ccaccgagat ggtactgccg caggtggcgg catccaacgt ggtgatggcc acgctggggc
      781 cttccacggg agttatggtc atagtggccg tatcgggaca ggccggcgag gtctgggtcg
      841 aggcgatcag gctcacggtg aaggtggtcg tggaagggaa cgtgtacacc aggtcctcca
      901 cggtgttgtt cggcacaccg ctgccatcca cgagccattc ccaatcaaaa acaccaccgc
      961 tggtattgtt cagcgttacg gacgcccctt cgcacggggt accagggagg
//
LOCUS       DAOQOC010001301         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_158133_length_1009_cov_4.253669, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001301 DAOQOC010000000
VERSION     DAOQOC010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158133_length_1009_cov_4.253669"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PL106_16330"
     CDS             <1..556
                     /locus_tag="PL106_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA34699.1"
                     /translation="DAMKKAGYATDPRYPDKLIELIERYELHKLDAGIDVSYAQKPSS
                     KPATTTSKGSHHRSNDLGTVTIGGGREVLLSDSRVKYVKARKGETIARIAADLEQMPG
                     WIAGWNDLGKNEPLEEGQTIYIQPKRNKAKHAEFHLAEQDETLWGISQRYGVKLKKLA
                     AYNGIGIADPLTPGQRVWLRKPRH"
BASE COUNT          225 a          327 c          281 g          176 t
ORIGIN      
        1 cgacgcgatg aagaaggccg ggtacgccac cgatccgcgc taccccgaca aactgatcga
       61 actgatcgaa cgctacgaac tgcacaagct cgatgccggt atcgatgtga gctatgcgca
      121 gaagccctca tccaagccgg caactaccac cagcaaggga tcgcaccacc ggtcgaatga
      181 cctcggcacc gttaccatcg gtggtggccg cgaagtgctc ctttccgaca gccgcgtgaa
      241 gtacgtgaag gcgcgcaagg gcgagaccat cgcacgcatc gcggccgacc tggagcagat
      301 gcccggctgg atcgccggct ggaacgacct gggcaagaac gagccgctcg aagaaggaca
      361 gaccatctac atccaaccca agcgcaacaa ggccaagcat gccgagttcc acctggccga
      421 acaggacgaa acgctctggg gcatcagcca gcgctacggt gtgaagctga agaagctggc
      481 cgcgtacaac ggcatcggta tcgccgatcc gctgacgccc ggccagcgcg tgtggttgcg
      541 caagccgagg cactgaacgc cactttggtc ttgtttcgca cttctgaggt tccctgcacg
      601 cagcgtatac cgcccggatc ggcgaaaatg aattgaggag caggagcccc gctcctcttc
      661 agtcgattct tggcccccct tgaagatgta ataccaattc gaatttcaat accaatggag
      721 ctgcttcttc acccaagttg gaggacacac tctccggatg ccttctccgt agagcagctt
      781 ccgtaggccg ctctgtcgcc acagcggtta tggcgcgtcg gaggcccgct acgcaacctg
      841 ctcagcgtga cagttccacg ggagtgttca agaacagtgc tcttggacac tcatgtcccc
      901 actgtcacgc tgagcgagcc tgccctgacg caggaagggc cgaagcgcca tactcccgtt
      961 ctcggcgttc gttccggatc ttgaccacaa acacttgccc tcaccgatc
//
LOCUS       DAOQOC010001302         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_158164_length_1009_cov_3.406709, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001302 DAOQOC010000000
VERSION     DAOQOC010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158164_length_1009_cov_3.406709"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PL106_16335"
     CDS             complement(<1..653)
                     /locus_tag="PL106_16335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNA34700.1"
                     /translation="MASSNKNGTRPIDLRFLEGPRSRWQEFRTALSIAGEFIRGFRKL
                     HFLGPCVTFFGSARFTEDHPYYSATRELARRIGRVGFNIMSGGGPGLMEAANRGARDV
                     GALSVGCNIVLPHEQHANPYLDVSLTFDRFFVRKVLLVKYSICFVVMPGGAGTVDELF
                     ETITLIQTGKVRDFPILLYGREYWRPMLDQIDRMVAEGTIGRKELEFLFVADNVEEAT
                     "
     gene            823..>1009
                     /locus_tag="PL106_16340"
     CDS             823..>1009
                     /locus_tag="PL106_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34701.1"
                     /translation="MKHLITLTFTLGATLTFAQPQIINSGFETWQDPDAAVAEPEEWS
                     SVKTSDASEVVNNLAPQL"
BASE COUNT          211 a          325 c          288 g          185 t
ORIGIN      
        1 gtggcctcct ccacgttgtc cgccacgaaa aggaattcga gttccttgcg tccgatggtg
       61 ccttcagcga ccattctgtc gatctgatcg agcatcggtc gccagtattc cctgccgtac
      121 agaagaatgg ggaaatcacg caccttgccc gtttggatga gcgtgatggt ctcgaacagt
      181 tcgtccaccg tgcccgcacc accgggcatc accacgaaac aaatactgta cttcaccaag
      241 agtaccttcc tgacgaaaaa gcggtcaaag gtgaggctga cgtccaggta cggattggca
      301 tgctgctcgt gcgggaggac gatgttgcag cccacactga gcgcgccaac atcacgtgct
      361 ccgcggttcg cggcctccat caggcccggg ccaccacccg acatgatgtt gaagcccacg
      421 cgtccgatgc gccgcgccaa ctcgcgcgtt gcggagtagt acggatggtc ctccgtgaag
      481 cgtgcactgc cgaaaaaggt gacgcaaggt cccaggaaat gcagcttgcg gaatccacgg
      541 atgaactctc cggcgatgga cagtgcggtc cggaactcct gccaccggga ccgtggacct
      601 tccaggaagc ggaggtcgat tgggcgagtg ccattcttgt tcgatgatgc catacgcgca
      661 cggatcacat gggacggatc gggggcgaaa ctacccggca aggtcctacg accgccaggc
      721 gcctgttccg gtacaatttc acaccctccg cccgccggcg tcccgtgcat cgcatacgca
      781 gccctggttc cggtgctatg ttagcggccc atcaactccc gcatgaagca cctcattacc
      841 ctcacgttca cgctgggcgc cacgctcacc ttcgcccagc cgcagatcat caacagtgga
      901 ttcgagacct ggcaggatcc cgatgccgcc gttgcggaac ccgaggaatg gagttcggtg
      961 aaaaccagcg atgcgagcga agtggtcaac aacctcgccc cgcagctct
//
LOCUS       DAOQOC010001303         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_158168_length_1009_cov_3.317610, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001303 DAOQOC010000000
VERSION     DAOQOC010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158168_length_1009_cov_3.317610"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PL106_16345"
     CDS             <1..271
                     /locus_tag="PL106_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34702.1"
                     /translation="IDGIVSGRLMNGKGMRAAYFRTTAGLLVDLEPTDARVAKRMKGI
                     HLLFDPKDLLLRELRMEQANGDNTVTRFTGARAGAPLPANTFQLP"
     gene            271..648
                     /locus_tag="PL106_16350"
     CDS             271..648
                     /locus_tag="PL106_16350"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34703.1"
                     /translation="MNGLQTLFTLLDPPTVGTNNATVRVRIDAGHRLFSGHFPDRPVL
                     PGVVMAHIATLIISGMTGTPMRIVTARAIKFLSPVDPHLTPELTFTAELRPGDNDTGV
                     EVRATAGETTVMKLTASVIRDER"
     gene            638..>1009
                     /locus_tag="PL106_16355"
     CDS             638..>1009
                     /locus_tag="PL106_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA34704.1"
                     /translation="MSGNADLFAGICVVVPTFNNARTLEDVLERVEHVLRSEGTAAPC
                     VIVVDDGSTDDTSRILGKVKNIHVLRVPVNRGKGNALRRGFDAALKLGFRNAITIDSD
                     GQHDPADLPALARAIQDDPDAM"
BASE COUNT          197 a          314 c          328 g          170 t
ORIGIN      
        1 gatcgatggc atcgtgagcg ggcgactgat gaacgggaag ggcatgcgcg ccgcgtattt
       61 ccggaccacc gccggtttgc tggtggatct ggaacctacc gatgcacgcg tagcgaagcg
      121 catgaaaggg atccatctgc tcttcgaccc gaaggacctg ttgctgcgcg agctgcgcat
      181 ggaacaggcc aatggggata acacggtcac acgcttcacc ggtgcgcggg ccggggcgcc
      241 actgccggcg aacacgttcc aactgccctg atgaacggac ttcaaacgct tttcacgctg
      301 cttgacccac cgaccgtggg cacgaacaac gcaacggtac gcgtgcggat cgatgccggt
      361 caccggctct tcagcgggca ctttcccgat cggcccgtgt tgccgggcgt ggtgatggcg
      421 cacatcgcca ccctgatcat cagtggaatg accgggaccc cgatgcgcat cgtgaccgca
      481 cgcgccatca aattcctcag tcccgtggat ccgcacctga cgccggagct gaccttcacc
      541 gcagaattgc gacccgggga caacgatact ggtgtcgagg tacgggcgac ggccggggag
      601 accacggtga tgaagctcac ggcatcggtg atccgtgatg agcggtaacg cggacctgtt
      661 cgcgggcatt tgcgtggtgg tgcccacgtt caacaacgcg cgcacactgg aggatgtgct
      721 cgaacgggtg gagcacgtgc tccgatccga aggcaccgcc gccccgtgcg tgatcgtggt
      781 ggacgatggt tccacggacg acacgtcacg gatcctgggc aaggtgaaaa acatccatgt
      841 gttgcgggtg ccggtgaacc gcggcaaggg caatgccttg cgcaggggat tcgacgcggc
      901 cttgaagctc ggtttccgca acgcgatcac catcgacagc gatggccagc acgacccggc
      961 cgacctgcct gccttggcac gtgccataca ggatgacccc gacgccatg
//
LOCUS       DAOQOC010001304         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_158174_length_1009_cov_3.198113, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001304 DAOQOC010000000
VERSION     DAOQOC010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158174_length_1009_cov_3.198113"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PL106_16360"
     CDS             <1..402
                     /locus_tag="PL106_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNA34705.1"
                     /translation="CSSGSGRNYQGEGVMSIAHAFLGAGTKSVVHTLWPVDDRSTSEI
                     LQDFYVGLEDDLPASEALAKAKLDFIARHAHDGLADPFYWSGIVLTGTDVVLQSEPRS
                     DWWYATAALPLLAGGYILSKRRSRARAWGET"
     gene            complement(351..>1009)
                     /locus_tag="PL106_16365"
     CDS             complement(351..>1009)
                     /locus_tag="PL106_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34706.1"
                     /translation="VDFEKEMADDPSLRSDVDATRRAIEGLRTLGEERLRQELKNADA
                     EVAGAPGASRTRWWWAAAAVLLLGGLAWWLVPTRDTPQALADEFRWSEPGLPVLMGTS
                     PRAMDAIMNAYKQEDFGTANSLLAAALERDPRNDTLQYFRGVLQDRVNGCASAEAWYA
                     QVHTTSVFASKAGYGLALCALKQGDLSLAREQLRRVVAMGDPQVSPQARALLLRLDKI
                     "
BASE COUNT          186 a          343 c          296 g          184 t
ORIGIN      
        1 tgttccagtg gcagtggccg caactaccag ggtgaagggg tgatgagcat cgcccacgcg
       61 ttcctcggcg cgggaacaaa gtcggtggtg catacgctct ggcccgtcga tgatcgctca
      121 acaagcgaga tcctccagga cttctacgtc ggtctcgagg atgaccttcc agcttcggag
      181 gcgctcgcca aagcgaagtt ggacttcatc gcacgccatg cccacgacgg tctcgcggat
      241 ccattctact ggagcggtat cgtactcact ggtacggacg tggtgctgca gtccgaaccc
      301 cgaagcgatt ggtggtacgc caccgctgca ttgccactcc tcgccggagg ctatatttta
      361 tccaaacgaa gaagtagagc gcgcgcttgg ggcgaaacct gaggatcgcc catcgccacc
      421 acccgccgca gttgctcacg cgcaagggac aggtcgccct gcttgagcgc acacaacgcc
      481 aggccgtatc ccgccttcga ggcgaacacg gaagtggtgt gaacctgcgc gtaccatgct
      541 tcggccgacg cgcatccgtt cacccggtcc tgcagcacac cacggaagta ctgaagtgtg
      601 tcgttccgcg gatcccgctc aagtgccgcc gcaagcaggc tgttcgccgt gccgaagtcc
      661 tcctgtttgt atgcattcat aatggcgtcc atggcgcggg gcgatgtccc catgagcacc
      721 ggcaagcccg gctcggacca ccggaattca tcggccaatg cctggggtgt atcgcgcgtg
      781 ggcaccagcc accacgccag gccgccgagc agcagtaccg ccgctgcggc ccaccaccaa
      841 cgggtgcggc tcgctcctgg cgccccagca acttccgcat ccgcattctt cagttcctgg
      901 cgcagtcgct cctcgcccaa ggttcgcaag ccttcgatgg cgcggcgtgt ggcatccaca
      961 tcggaacgca agctcgggtc gtcggccatc tccttctcga agtccacac
//
LOCUS       DAOQOC010001305         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_158804_length_1007_cov_4.630252, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001305 DAOQOC010000000
VERSION     DAOQOC010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158804_length_1007_cov_4.630252"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PL106_16370"
     CDS             <1..330
                     /locus_tag="PL106_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34707.1"
                     /translation="GYYLNQRGQPTFDNGQVKRTGSVVNGRNQGRWTWYYPDGRKKME
                     GDFAGGKRTGRWITFASNGDTLTVSMYRNDQLNGLHTDYGPDGRPTLSTNYVNDKPTG
                     PVDPATQ"
     gene            581..>1007
                     /locus_tag="PL106_16375"
     CDS             581..>1007
                     /locus_tag="PL106_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34708.1"
                     /translation="MLFLNTLAFTTCFAAWMLNGVLVTYLVDHQVFNWGPVEVGWLLG
                     IPVLTGSIFRLPLGMLTDRYGGRPVFTALLLFCSIPMYLLSEVNSFAGFAAMSFGFGI
                     IGTGFAVGIAFTSVWYPKRQQGLALGVFGAGNAGAALTTL"
BASE COUNT          193 a          300 c          298 g          216 t
ORIGIN      
        1 ggttactact tgaaccagcg tgggcagccc acgttcgaca acggccaggt gaagcgtacg
       61 ggcagcgtgg tgaacgggcg caatcaaggc cgctggacct ggtactatcc cgatggccgc
      121 aagaaaatgg agggtgactt cgccggcggc aagcgcacgg gccgctggat caccttcgcc
      181 tccaatggcg atacactcac cgtatccatg tacaggaatg accaattgaa cgggctgcac
      241 acggactacg gtccggatgg aaggcccacc ttgtcgacca attatgtgaa cgacaagccg
      301 acaggcccgg tggatccggc gacgcaatag ctcccgtacg ggtcgagacc cgatttatac
      361 cttattatat ccatatccgt actcaagttc cgggcggagt ccttctgtgg ccaccctggg
      421 ccgacgatca gtggaggatc cattgaaaaa tttgacattc tatgatataa atcattataa
      481 taattattag ttgttcgtcc tttgatccgg cgctggggcg ctcccctatc ccctggcgag
      541 ccaatgaccc atggagcgga ctacaggaag tgctcaccgt atgttgttcc tgaacacgct
      601 ggcgttcacc acctgcttcg cggcatggat gctcaacggg gtgctggtca cctacctggt
      661 ggaccatcag gttttcaact ggggacccgt ggaggtgggc tggctgctgg gcatcccggt
      721 gctcaccggt tcgatcttcc gcctgcccct gggcatgctc accgaccgct acggggggcg
      781 gccggtgttc accgccctgc tgctcttttg ctccatcccc atgtacctgc tctcggaggt
      841 gaacagcttc gcgggcttcg cggcgatgag cttcggcttc gggatcatcg gaacgggctt
      901 cgcagtaggc atcgcattca cttcggtctg gtatcccaag cgccagcaag gcctcgcgct
      961 gggggtgttc ggcgcgggca acgctggagc agcgctcacc acgcttt
//
LOCUS       DAOQOC010001306         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacteriales bacterium isolate AMR_MDS_0480
            NODE_160537_length_1002_cov_3.470961, whole genome shotgun
            sequence.
ACCESSION   DAOQOC010001306 DAOQOC010000000
VERSION     DAOQOC010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810112
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacteriales bacterium (wastewater metagenome)
  ORGANISM  Flavobacteriales bacterium
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:43:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,276
            CDSs (total)                      :: 3,252
            Genes (coding)                    :: 3,237
            CDSs (with protein)               :: 3,237
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 0 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Flavobacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160537_length_1002_cov_3.470961"
                     /isolate="AMR_MDS_0480"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2021391"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..975
                     /locus_tag="PL106_16380"
     CDS             214..975
                     /locus_tag="PL106_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA34709.1"
                     /translation="MQPPLKEEGEFKYIEVGDGPPLLFLHGLFGALSNFEGPIDFFGK
                     KYRVVMPMLPLYTLPMLNTNIPALAEFLDRFIRHKGFTEVNLLGNSLGGHIALVYCTK
                     HMATVRTLILTGSSGLYENAFGGGFPRREDKEYLRAKIALTFYDPKHTTDALVEECYT
                     TVNDKGKLIRILSLAKSAIRHNMAKDLPNLKMPVALIWGRQDTITPPHVAEEFNTLIP
                     GSELFWIDECGHAPMMEHPNTFNRLVDEWLAKTLA"
BASE COUNT          213 a          304 c          309 g          176 t
ORIGIN      
        1 gccccttcgc cgagcgcgag caggctgtcc acctcactac gcaccagcag gtcgcgcagt
       61 atgggcaggg cgttctggcc gtacgccgca ccgcccagga ggaccgtcca gaccaccaac
      121 gcgcgcatgc cggatcgcat gtggacaaga tagtgtgtgg gatcggatgg tgctcgacga
      181 agtgaagcgt ttacatttgg tcgcaccctg cgcatgcaac cgccgctgaa ggaagaagga
      241 gagttcaagt acatcgaggt cggtgacggt ccgccgttgc tgttcctgca cggcttgttc
      301 ggtgcgctca gcaacttcga agggcccatc gactttttcg ggaagaagta ccgcgtggtg
      361 atgccgatgc tgccgctgta cacgctgccc atgttgaaca ccaacattcc ggcactggcg
      421 gaattcctcg atcggttcat ccggcacaag ggattcacgg aggtgaacct gctgggcaac
      481 tcgctcggcg gccatatcgc gctggtgtac tgcaccaagc acatggccac ggtgcgcacg
      541 ctgatcctca ctggcagcag cggcctgtac gaaaatgcgt tcgggggcgg ctttccgcgc
      601 cgcgaggaca aggagtacct ccgcgccaag atcgcgctca ccttctacga tcccaagcac
      661 accacggacg cgctggtgga ggagtgttac accaccgtga acgacaaggg caagttgata
      721 cggatcctgt cgctggccaa gagcgcgatc cgccacaaca tggccaagga cctgccgaac
      781 ctcaagatgc ccgtggcact catctggggc aggcaggaca cgatcacacc gccgcatgtc
      841 gcggaggaat tcaacacctt gatccccggc agcgagttgt tctggatcga tgaatgcgga
      901 cacgccccga tgatggaaca cccgaacacc ttcaaccggc tggtggatga atggctggcg
      961 aagacgctcg cttgaaatgc caatggtgag tagcgagtgg cg
//
